bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibitory effect of lncRNA SNHG16 Knockdown on G2/M Transition and Proliferation of Gallbladder Carcinoma Cells via miR-3138/CDC25B/CDK1 Axis Running title: SNHG16’s mechanisms in Gallbladder cancer
Authors: Li Yun , Zhao Lujie , Wang Tong , Pan Jiquan , Wang Jingye , Chen Shuxin , Tian Yahui , Yang Zhongfa , Zou Xiangyu , Peng Fujun , Li Shengguang 
Published: September 2022

Abstract
Background

Gallbladder cancer (GBC) is the most common and aggressive malignant tumor in the biliary system, and its tumorigenesis and development are associated with abnormal expression of the long non-coding RNA (lncRNA). Although several studies have demonstrated that SNHG16 plays an important role in various tumors, its mechanism in GBC has not been systematically investigated.

Methods

This study is described from three aspects, including tissue, cell lines, and animal model. The qRT-PCR assay was applied to calculate the mRNA levels of SNHG16, miR-3138, and CDC25B. The link between SNHG16, miR-3138, and CDC25B expression was verified with the dual-luciferase reporter assay, RNA-binding protein immunoprecipitation assay (RIP), rescue assay, gene expression correlation analysis, etc.

Results

SNHG16 was highly expressed, and miR-3138 was lowly expressed in GBC tissues and cells compared to normal tissues and cells. Low expression of SNHG16 prevents the G2/M transition of the cell cycle by inhibiting the dephosphorylation of CDK1, inducing cell apoptosis, and suppressing cell proliferation in vitro and in vivo. LncRNA SNHG16 directly binds to miR-3138 and regulates miR-3138 expression in GBC-SD and NOZ cells. Cell proliferation and G2/M transition were inhibited by mimics of miR-3138. miR-3138 mediated CDC25B/CDK1 expression by binding directly to CDC25B mRNA. Importantly, the miR-3138 inhibitor could partially reverse the effect of SNHG16 knockdown on cell proliferation, G2/M transition, and cell apoptosis. SNHG16 knockdown reduced CDC25B expression in GBC-SD and NOZ cells.

Conclusion

As a competitive endogenous RNA, SNHG16 participates in the molecular regulatory network of the GBC cell cycle and proliferation through the mir-3138/CDC25B/CDK1 axis, enhancing the understanding of the pathogenesis of gallbladder cancer.
Hyepereosiniphilic syndrome and COVID-19 pandemic: 2 case reports
Authors: Sherafati Alborz , Rahmanian Mehrzad , Badkoubeh Roya Sattarzadeh , Khoshavi Meysam , Foroumandi Morteza , Peiman Soheil , Shahi Farhad , Sardari Akram , Pourkia Roghayeh , Larti Farnoosh 
Published: September 2022

Abstract
Background
Nearly half of the patients with hypereosinophilic syndrome (HES) have cardiovascular involvement, a major cause of mortality. COVID-19 infection can lead to cardiac involvement, negatively impacting the clinical course and prognosis. We reported two cases of HES with cardiac involvement resulting in valve replacement, which were complicated by COVID-19.
Case presentation:
Our first case was a 27-year-old woman admitted due to dyspnea and signs of heart failure. An echocardiogram revealed severe mitral stenosis and mitral regurgitation. Corticosteroid therapy resulted in the improvement of her symptoms. However, she deteriorated during her hospital course following a positive COVID-19 test. A repeated echocardiogram showed right ventricular failure with severe mitral regurgitation and torrential tricuspid regurgitation. Therefore, she underwent mitral and tricuspid valve replacement. Our second case was a 43-year-old man with HES resulted in severe tricuspid stenosis. Corticosteroid treatment improved tricuspid stenosis, but he underwent valve replacement due to severe valvular regurgitation. He was admitted following tricuspid prosthetic valve thrombosis. Initial workups revealed lung involvement in favor of COVID-19 infection, and his PCR test was positive.
Conclusion
COVID-19 pandemic can change the clinical course of HES. It may result in a heart failure exacerbation due to myocardial injury and an increased risk of thrombosis in prosthetic valves or native vessels due to hypercoagulability.
Replaced donor right hepatic artery microsurgical reconstruction with interpositioning of the superior mesenteric artery in pediatric deceased donor liver transplantation
Authors: Li Zhiwei , Zhang Wei , Shen Yan , Bai Xueli , Liang Tingbo 
Published: September 2022

Abstract
Purpose
To present our experience of using the pediatric donor superior mesenteric artery (SMA) as the interpositioning vessel to reconstruct the hepatic artery in pediatric deceased donor liver transplantation.
Methods
Pediatric patients who received pediatric deceased donor livers from December 2019 to June 2021 were enrolled.
Results
A total of 43 pediatric recipients received pediatric deceased donor livers during the study period. Thirty-four (79.1%) pediatric donors had normal hepatic artery anatomy, while nine (20.9%) showed variant anatomies. The SMAs of the pediatric donors were interposed in the latter eight cases. The anastomosis between the donor distal SMA and the donor celiac trunk artery (CTA) was initially performed in the back-table period, after which the other anastomosis between the donor proximal SMA and recipient CHA was performed. Only one case showed HAT occurrence (incidence rate, 2.3%). However, no arterial complications occurred in pediatric recipients with donor grafts that showed the variation of RHA replacement from the SMA and had undergone usage of the donor’s SMA as an interpositioning vessel.
Conclusion
In pediatric deceased donor liver transplantation, the use of SMA as an interpositioning medium can solve the problems related to a tiny caliber and anatomical variations of the donor hepatic artery.
Why Should Big Data-based Price Discrimination be Governed?
Authors: Lu Fang , Xing Genshang , Wang Qin 
Published: September 2022

Abstract
The e-commerce platform provides data service for resident merchants for precise marketing, but which also leads to frequent occurrence of big data-based price discrimination by merchants. To govern big data-based price discrimination, it is necessary to clarify the motivation. In this paper, by constructing a differential game model based on merchant reputation dynamics, the impact of big data-based price discrimination on the data service level, merchant reputation, consumer surplus and benefits of the platform and merchant are researched, the reason why it is difficult to govern big data-based price discrimination is analyzed, and relevant suggestions are put forward. The research shows that: based on revenue factors, the platform will not give up providing data service to the merchant because of merchant's big data-based price discrimination, while the merchant tend to adopt the big data-based price discrimination strategy in order to obtain additional income, which leads to generate a vicious closed loop that is difficult to eliminate big data-based price discrimination. To eliminate the vicious closed loop, third-party supervision should be introduced into the platform; compared with big data-based price discrimination, when precision marketing has a greater impact on reputation and demand, resident merchants tend to adopt a combination of precision marketing and big data-based price discrimination strategies. Therefore, the platform should provide appropriate data service levels for different industries, so as to avoid high-quality data service from becoming a tool for merchants to conduct big data-based price discrimination strategy; big data-based price discrimination will reduce the level of platform's data service, consumer surplus and platform profits, but the merchant's profits will increase significantly, so to curb the big data-based price discrimination, it is necessary to vigorously increase the punishment for the merchants.
Preliminary Design of Insulation System for Superconducting Conductor Testing Facility
Authors: Yuanyuan Ma , Xiaochuan Liu 
Published: September 2022

Abstract
The Superconducting Conductor Testing Facility, which developed to evaluate the reliability of engineering technology and safe operation in fusion reactor operation environment., is under engineering design by ASIPP. In case of an emergency shut-down like in succession of a quench, the voltage across the coil may rise to about 2.5 kV. Therefore, the facility has to be provided with a reliable insulation. This paper introduces the preliminary design of the insulation system. The insulation system of Superconducting Conductor Testing Facility includes turn and ground insulation. Since the magnet of the facility adopts the manufacturing process of "Wind&React ", so its turn insulation material needs to withstand a heat treatment temperature of 650℃. The insulation system is composed of S-glass fiber reinforced tape and vacuum-pressure impregnated in a DGEBF epoxy system. The mechanical properties are being subjected to investigations with respect to the design requirements and operating conditions. The test results are analyzed to verify the feasibility of insulation system design.
Identification of Cutibacterium acnes in spondylitis by metagenomics analysis
Authors: Huang Yao-Ting , Yang Meng-Yin , Mao Yan-Chiao , Lee Ding-Yu , Kuo Yu-Lun , Tseng Chien-Hao , Liu Chia-Wei , Fu Yun-Ching , Liu Po-Yu 
Published: September 2022

Abstract
Background:
Identifying pathogens with culture-negative pyogenic spondylitis was difficult. Shotgun metagenomic sequencing is an unbiased and culture-free approach to the diagnosis of infectious diseases. There were, however, a variety of contamination factors that confounded the precision of metagenomic sequencing.
Methods:
This study proposed a contamination-robust protocol for clinical metagenomic sequencing. Using this protocol, we were able to identify Cutibactierum modestum in tissue specimen of infectious spondylitis.
Results:
The identification of Cutibactierum modestum and annotation of its resistome were annotated. The patient recovered fully.
Conclusions:
This application of next-generation sequencing using the contamination-robust protocol to assist the diagnosis of Cutibactierum modestum infection provides a new perspective in the clinical approach against spinal osteomyelitis and illustrates the potential of this technique in rapid etiological diagnosis.
The nuclear receptor subfamily 4 group A1 in human disease
Authors: Wang Hongshuang , Zhang Mengjuan , Fang Fang , Xu Chang , Liu Jiazhi , Gao Lanjun , Zhao Chenchen , Wang Zheng , Zhong Yan , Wang Xiangting 
Published: September 2022

Abstract
Nuclear receptor 4A1 (NR4A1), a member of the NR4A subfamily, acts as a gene regulator in a wide range of signaling pathways and responses to human diseases. Here, we provided a brief overview of the current functions of NR4A1 in human diseases and the factors involved in its function. A deeper understanding of these mechanisms can potentially improve drug development and disease therapy.
Improvement of ADHD patients after 1 year from the time of diagnosis: differences between centers of a regional network
Authors: Cartabia Massimo , Finazzi Stefano , Bonati Maurizio 
Published: September 2022

Abstract
Although the pharmacological therapy of ADHD has been widely studied, little has been done to compare the different therapeutic approaches (e.g., drug therapy vs psychological treatments) and even less has been done to compare the outcome of the therapy between centers. This multicenter observational study aims to assess between-center variation in functional outcome of ADHD patients one year after the diagnosis, according to the treatment received. We used the Regional ADHD Registry data on 1429 patients enrolled in 16 ADHD centers in the 2011–2022 period. To evaluate the effectiveness of the therapy we used a generalized linear mixed model with the center as the random effect, including patient condition at diagnosis and center characteristics, weighting by the inverse of the propensity score of the treatment received by the patient. Between-center variation was expressed as the relative difference in odds-ratios between the observed and the expected number of patients whose condition improved, using the Clinical Global Impressions—Improvement Scale (CGI-I), and the relative 95% CI. Patients who received combined treatment were significantly more likely to improve compared to other treatment groups (65.5% vs 54.4% for methylphenidate alone, 53.4% for psychological treatment alone, or 40.5% for no therapy). Adjusted for patients and center characteristics, the odds ratio ranged from 0.78 (0.22–1.48 95% CI) to -0.66 (-1.12—0.24 95% CI). The mean expected probability of improvement after one year of therapy for an average patient with ADHD for each center was 47.7% in a center at the 25th percentile and 61.7% in a center at the 75th percentile of the outcome distribution after adjustments. The wide between-center variation in patient functional improvement one year after the diagnosis of ADHD could be largely explained by center-specific therapeutic approaches or attitudes. More careful and stringent adherence to the latest guidelines and evidence could reduce differences in responses between centers, as could formal and periodic audit programs within and between centers.
GANAD:A GAN-based method for network anomaly detection
Authors: Fu Jie , Wang Lina , Ke Jianpeng , Yang Kang , Yu Rongwei 
Published: September 2022

Abstract
Cyber-intrusion can lead to severe threats to network, i,e., system paralysis, information leaky, and economic losses. To protect network security, anomaly detection methods based on generative adversarial networks (GAN) for hindering cyber-intrusion have been proposed. However, existing GAN-based anomaly score methods built upon the generator network are designed for data synthesis, which would get unappealing performance on the anomaly detection task. Therefore, their low-efficient and unstable performance make detection task still quite challenging. To cope with these issues, we propose a novel GAN-based approach GANAD to address the above problems which is specifically designed for anomaly identification rather than data synthesis. Specifically, it first proposed for a similar auto-encoder architecture, which makes up for the time-consuming problem of the traditional generator loss computation. In order to stabilize the training, the proposed discriminator training replace JS divergence with Wasserstein distance adding gradient penalty. Then, it utilizes a new training strategy to better learn minority abnormal distribution from normal data, which contributes to the detection precision. Therefore, our approach can ensure the detection performance, and overcome the problem of unstable in the process of GAN training. Experimental results demonstrate that our approach achieves superior performance than state-of-the-art methods and reduces time consumption at the same time.
Correlation of PLVAP protein expression with microbial composition, clinicopathological features and prognosis of patients with stomach adenocarcinoma
Authors: Wen Yuting , Wang Yi , Huang Yao , Liu Zhe , Hui Chan 
Published: September 2022

Abstract
Purpose Plasmalemma vesicle-associated protein (PLVAP) is involved in many immune‑related signals; however, its role in stomach adenocarcinoma (STAD) remains to be elucidated. This study investigated PLVAP expression in tumor tissues and defined the value in STAD patients.
Methods A total of 96 patient paraffin-embedded STAD specimens and 30 paraffin-embedded adjacent non-tumor specimens from the Ninth Hospital of Xi’an were consecutively recruited in analyses. All available RNA‑sequence data from the Cancer Genome Atlas database(TCGA). PLVAP protein expression was detected using immunohistochemistry. Microbial community analysis was performed by 16S rRNA gene sequencing using Illumina MiSeq. PLVAP mRNA expression was explored with the Tumor Immune Estimation Resource (TIMER), GEPIA, and UALCAN databases. The effect of PLVAP mRNA on prognosis was analyzed via GEPIA, and Kaplan-Meier plotter database. GeneMANIA and STRING database were used to predict gene/protein interactions and functions. The relationships between PLVAP mRNA expression and tumor-infiltrated immune cells were analyzed via the TIMER and GEPIA databases.
Results Significantly elevated transcriptional and proteomic PLVAP expressions were found in STAD samples. Increased PLVAP protein and mRNA expression were significantly associated with advanced clinicopathological parameters and correlated with shorter disease free survival (DFS) and overall survival (OS) in TCGA (p<0.001). The microbiota in the PLVAP-rich (3+) group was significantly different from that in the PLVAP-poor (1+) group(p<0.05). The results from TIMER showed that high PLVAP mRNA expression had significant positive correlations with CD4+ T cell (r =0.42, p < 0.001).
Conclusion PLVAP is a potential biomarker to predict the prognosis of patients with STAD, and increased PLVAP detection correlates with the relative abundance of bacteria.
Transcriptome Profiling Revealed the Relationship Between Immune-Related Genes and m6A Modifiers in Polycystic Ovary Syndrome
Authors: Kong Fan-Sheng , Zhou Yuan , Lu Yinghua , Ren Chun-Yan , Chen Min , Jia Ruofan , Chen Jian-Huan , Ma Yaping 
Published: September 2022

Abstract
Background
Polycystic ovary syndrome (PCOS) is a common female endocrinal disease that may result in excessive androgen, but the mechanism of PCOS remains unclear. Most patients with PCOS suffer from low-grade inflammation, whereas the underlying connection between immune and PCOS is still uncertain.
Objective
This study aimed to determine the immune-related mechanisms behind PCOS pathogenesis and explore distinct immune-related genes and their functional signatures in PCOS.
Methods
The microarray dataset GSE155489 was downloaded from the Gene Expression Omnibus (GEO) database. The immune-related genes were downloaded from ImmPort. The immune-related differential expression genes (IRDEGs) in PCOS were screened, and functional and pathway enrichment analyses were applied. The protein-protein interactions (PPI), module analysis, and transcription factor enrichment analysis (TFEA) were used to identify hub genes. The immune profile analysis was depicted, and the expression correlation analysis between hub genes and m6A modifiers in PCOS was constructed.
Results
125 IRDEGs were identified, and immune-related pathways included the cytokine-cytokine receptor pathway, T cell receptor signaling pathway, and TNF signaling pathway. All genes were associated with four immune cells (monocyte cells, nTreg, iTreg, and Tcm). Moreover, Major Histocompatibility Complex, Class I, A (HLA-A), Major Histocompatibility Complex, Class I, B (HLA-B), Fos Proto-Oncogene (FOS), Prostaglandin-Endoperoxide Synthase 2 (PTGS2), and C-X-C Motif Chemokine Receptor 4 (CXCR4) were identified as hub genes. Furthermore, N6-Methyladenosine (m6A methylation) mediators could potentially play a pivotal role between the immune system and PCOS.
Conclusion
This study described the relevance between immune and PCOS. We identified five IRDEGs as hub genes for PCOS. The relationship between the m6A methylation and hub genes indicated that m6A methylation could play a potential role in regulating such hub genes in PCOS. These findings could provide new insights into the molecular mechanisms and diagnosis or treatment strategy for the disease.
Inactivation of the antidiabetic drug acarbose by human intestinal microbial-mediated degradation
Authors: Jiang Weihong 
Published: September 2022

AbstractGut microbiota plays an important role in drug modification, transformation and degradation, which are factors that need to be considered in personalized therapy. Acarbose, an inhibitor of α-glucosidase, is used as a first-line drug for the treatment of type 2 diabetes; however, its clinical effects vary greatly among individuals, and the underlying mechanisms relating to the contribution of gut microbes remain to be elucidated. Herein, we reported the association between acarbose resistance and enteric bacteria by analyzing clinical samples and anaerobic enrichment culture, and isolated a major acarbose-degrading gut strain,Klebsiella grimontiiTD1. Metagenomic analysis of the intestinal microbiome found that the abundance ofK. grimontiiTD1 was higher in patients with weak acarbose response and increased with longer medication time. In addition,in vivoexperiments using a diabetic mouse model showed that this strain significantly weakened the hypoglycemic effect of acarbose. Further, we identified a key acarbose-preferred glucosidase, Apg, inK. grimontiiTD1 using a combined approach of induced transcriptome and protein profiling. This enzyme is structurally characteristic, could degrade acarbose into small molecules with loss of inhibitor function, and was widely distributed in intestinal microorganisms, especially inKlebsiella. These results indicate that the risk of acarbose resistance caused by metabolic degradation from intestinal bacteria is widespread in humans, and this "induced degradational inactivation" mechanism may be one of the main causes of non-antibiotic drug resistance.
How family precepts delivered enduring prosperity to merchants in early-modern Japan: A microeconomic perspective
Authors: Sasaki Katsuro , Ono-Yoshida Rie 
Published: September 2022

Abstract
Many wealthy merchants in the Edo period subscribed to the ideology emphasizing “family survival” as their management goal and made family precepts summarizing business principles toward this to pass on to their descendants. Many studies, till date, on the management of long-lived companies, including the family precepts of their ancestors, have attempted to identify their reasons for longevity and apply the learning to modern corporate governance. However, there is little research on how the ideology was generated and established. In this study, we apply the finite-term overlapping generation model to discuss how a merchant in each generation managed their inheritance to maximize their utility. In the model, we assume that wealthy merchants’ utility depends on their consumption and their progeny’s utility, and introduce a new parameter representing the degree of empathy the predecessor has toward their successor. Family precepts must have been elaborated to assure wealthy merchants' optimal mechanism of inheritance. Our result suggests that family precepts are key to determining the extent and duration of a merchant family's prosperity. In conclusion, if wealthy merchants establish family precepts that are steadily passed on to their descendants, the ideology emphasizing “family survival” and a commitment to frugality and thrift, the family will last long and prosper. Our results reinforce the conclusion of previous studies that family precepts had a significant role in the family's survival and the development of its human resources.

JEL Classification N85・D90