bluuebunny/crossref_metadata_2025_split huggingface dataset:
Smoking in Patients With Chronic Cardiovascular Disease During COVID-19 Lockdown
Authors: Chagué Frédéric , Boulin Mathieu , Eicher Jean-Christophe , Bichat Florence , Saint-Jalmes Maïlis , Cransac Amélie , Soudry Agnès , Danchin Nicolas , Laurent Gabriel , Cottin Yves , Zeller Marianne 
Published: April 2022

ObjectivesThis cross-sectional study aims to investigate health-related behaviors including tobacco consumption among patients with cardiovascular diseases (CVD), during the first COVID-19-related lockdown.MethodsAfter 5 weeks of COVID-19 lockdown, 220 patients with chronic coronary syndromes (CCS) and 124 with congestive heart failure (CHF) answered a phone questionnaire.ResultsAmong these 344 patients, 43 (12.5%) were current smokers, and none had quit during the lockdown. When compared with non-smokers, smokers were 15 years younger, more often diabetic, more likely to live in an urban than a rural lockdown location, and more often in the CCS cohort (p = 0.011). Smokers described greater psychological impairment, but their rates of decrease in physical activity and of increase in screen time were similar to non-smokers. More than one-third (13/43) increased their tobacco consumption, which was mainly related to stress or boredom, but not driven by media messages on a protective effect of nicotine.ConclusionsDuring the first COVID-19 lockdown, we found a decrease in favorable lifestyle behaviors among patients with CVD. Strikingly, one-third of smokers with CCS or CHF increased their tobacco consumption. Given the major impact of persistent smoking in patients with CVD, this highlights the need for targeted prevention strategies, in particular during such periods.
Non-alcoholic Fatty Liver Disease Is Associated With Cardiovascular Outcomes in Subjects With Prediabetes and Diabetes: A Prospective Community-Based Cohort Study
Authors: Song Qi-Rui , Liu Shuo-Lin , Bi Ya-Guang , Chen Shuo-Hua , Wu Shou-Ling , Cai Jun 
Published: April 2022

BackgroundThere have been no studies of the effect of non-alcoholic fatty liver disease (NAFLD) on cardiovascular events (CVEs) in patients with pre-diabetes (pre-DM), and diabetes mellitus (DM). We performed a community-based cohort study to evaluate the relationship between NAFLD and CVEs in patients with glucose metabolism disorder.MethodsWe enrolled 71,852 participants from the Kailuan study who had not experienced CVEs, after excluding alcohol abuse and other liver diseases. NAFLD was assessed using abdominal ultrasonography. Besides, participants were categorized by glucose metabolism status [normal glucose regulation (NGR), pre-DM, and DM]. All subjects were followed up for the occurrence of CVEs.ResultsDuring a median of 13.01 (0.64) years of follow-up, 6,037 CVEs occurred. NAFLD was present in 22,525 (31.3%), and compared with participants without NAFLD, those with NAFLD had a 12.3% [95% confidence interval (CI) 1.059–1.191, P &lt; 0.001] higher risk of CVEs, after adjustment for potential confounders. The hazard ratios for patients with mild, moderate, and severe NAFLD were 1.104 (95% CI 1.035–1.179, P &lt; 0.001), 1.149 (95% CI 1.055–1.251, P &lt; 0.001), and 1.235 (95% CI 1.059–1.441, P &lt; 0.001), respectively. Moreover, participants with pre-DM plus NAFLD and participants with DM plus NAFLD had 1.267-fold (95% CI 1.151–1.395, P &lt; 0.001) and 1.829-fold (95% CI 1.666–2.008, P &lt; 0.001) higher risks of CVEs, respectively, compared with those with NGR and no NAFLD. The addition of the combination of NAFLD and glucose metabolism status to the crude Cox model increased the C-statistic by 0.0066 (0.0053–0.0080, P &lt; 0.001).ConclusionsNAFLD is associated with higher risks of CVEs. Moreover, NAFLD is an independent predictor of CVEs in patients with pre-DM and DM, suggesting that NAFLD may provide greater risk predictive value for patients with glucose metabolism disorder.
Quantifying Stimulus-Affected Cinematic Spaces Using Psychophysiological Assessments to Indicate Enhanced Cognition and Sustainable Design Criteria
Authors: Sakhaei Hamidreza , Yeganeh Mansour , Afhami Reza 
Published: April 2022

Objectives: Converging architecture with cinema and cognition has proved to be a practical approach to scrutinizing architectural elements’ significant contribution to engineering science. In this research, a behavioral analysis has been conducted to examine if disruptive events in cinematic spaces can lead to an insightful perception of architectural qualities and enhanced interplay with the observed spaces to highlight mental health and improved cognitive tasks in sustainable design characteristics.Methods: The experiment was conducted in participants (N = 90) while watching three films with different stimuli to facilitate multivariate analyses. The HR, BP, SCL, and BT were measured while screening films to subjects. Psychological assessments of PANAS, TIPI, Chills, Pleasure, Arousal, Dominance, and NAQ were gathered to conduct correlation and regression analyses between variables. An independent space syntax analysis of film plans was also performed to compare film spaces’ properties.Results: Analyses show that physiological responses of HR, BP, SCL, and BT showed a meaningful relationship with the event intensity. Psychological assessments of Chills, SAM, and NAQ also depicted a meaningful relationship with the degree of stimuli during the movie screenings. Regression analyses illustrated that the age factor had a significant relationship with Arousal (p-value = 0.04), Chills (p-value = 0.03), and Dominance (p-value = 0.00). The TIPI factor showed a meaningful relationship with Chills (p-value = 0.03) and Dominance (p-value = 0.00). PANAS PA factor’s relationship was significant on Chills (p-value = 0.00), Arousal (p-value = 0.04), and Dominance (p-value = 0.03), and the PANAS NA factor showed a meaningful relationship with Chills (p-value = 0.00) and Dominance (p-value = 0.05). The correlations in Chills–Arousal (p-value = 0.01), PANAS NA–TIPI (p-value = 0.01), NAQ–Pleasure (p-value = 0.05), and Arousal–Dominance (p-value = 0.00) were significant. Space syntax analyses also showed that film 3 had a mixed plan structure than the other two films. Factors such as area compactness, connectivity, visual entropy, controllability, and mean depth were influential in distinguishing film spaces.Conclusion: It has been concluded that the space with intensive disruption of architectural elements successfully indicated improved cognitive perception of spatial qualities, enhanced interaction, and signified sustainable design criteria. Evoking events disrupted the banalization of cinematic spaces, illustrating that the designed model can indicate a more homogenous evaluation of a sustainable environment.
Biotechnological Applications of Manganese Peroxidases for Sustainable Management
Authors: Kumar Ashish , Arora Pankaj Kumar 
Published: April 2022

Microbial manganese peroxidases (MnPs) are ligninolytic enzymes primarily responsible for degrading lignin, but they are also capable of degrading dyes, organic pollutants, and emerging contaminants in wastewater. They have been isolated and characterized from many white-rot fungi and few bacteria. For microbial MnPs, the optimum pH range is between 3.5 and 9.0; the optimum temperature range is between 25°C and 70°C. Their molecular weights range between 25 kDa and 68 kDa. By oxidizing Mn2+ to Mn3+, they can oxidize a wide range of phenolic and non-phenolic substrates. MnPs can be used in a wide range of industrial applications, including delignification of pulp, wastewater treatment, biofuel production, dye removal, biopulping, biobleaching, and juice extract clarification. The purpose of this review is to summarize biotechnological applications of manganese peroxidases.
Identification of SSTR5 Gene Polymorphisms and Their Association With Growth Traits in Hulun Buir Sheep
Authors: Li Xue , Ding Ning , Zhang Zhichao , Tian Dehong , Han Buying , Liu Dehui , Liu Sijia , Tian Fei , Fu Dejun , Song Xiaoliang , Zhao Kai 
Published: April 2022

The aim of this study was to locate SSTR5 polymorphisms and evaluate their association with growth traits in Hulun Buir sheep. The study followed up 884 Hulun Buir sheep from birth to 16 months of age, which were born in the same pasture and the same year, and a consistent grazing management strategy was maintained. The birth weight (BRW) was recorded at birth, and body weight (BW), body height (BH), body length (BL), chest circumference (ChC), chest depth (ChD), chest width (ChW), hip width (HW), and cannon circumference (CaC) were measured at 4 and 9 months of age. BW, BH, BL, ChD, HW, and CaC were also recorded at 16 months of age. Based on the growth traits, 233 sheep were selected as experimental animals. Sanger sequencing was performed, and seven single-nucleotide polymorphisms (SNPs) were identified. Association analyses of the SNPs and the growth traits were then conducted. Seven SNPs of the SSTR5 exhibited moderate polymorphism (0.25&lt;PIC&lt;0.5) and were consistent with the Hardy–Weinberg equilibrium. SNP7 (T989C, rs601836309) caused a change in amino acid sequences, while others did not cause any change. The genotypes of SNP1 (C186T, s400914340) were significantly associated with BW, ChW, and ChC at 4 months of age and with HW at 9 months of age (p&lt;0.05). These genotypes also showed extremely significant association with CaC at 4 months of age (p&lt;0.01). The genotypes of SNP7 exhibited a significant association with ChW and CaC at 4 and 9 months of age, respectively. Moreover, the genotypes of SNP3 (T384C, rs413380618)) and SNP4 (T537C, rs605867745) were significantly associated with CaC at 9 months of age (p&lt;0.05). Linkage disequilibrium was observed among the seven SNPs with five haplotypes. However, these haplotypes were not associated with growth traits at different ages. In conclusion, SNP1, SNP3, SNP4, and SNP7 may serve as molecular markers for the growth traits of Hulun Buir sheep.
Case Report: DARS Mutations Responsible for Hypomyelination With Brain Stem and Spinal Cord Involvement and Leg Spasticity
Authors: Liu Meijun , Xiao Wen , Yang Fang , Wang Xueqing , Chen Chao , Jin Shuoguo , Ran Ningjing , Chen Weiyin , Yang Dongdong 
Published: April 2022

Objective: Hypomyelination with brain stem and spinal cord involvement and leg spasticity (HBSL) is a rare form of leukodystrophy presenting with varying clinical and imaging features. We report a case of HBSL to investigate the clinical and radiological characteristics of HBSL resulting from cytoplasmic aspartyl-tRNA synthetase gene (DARS) mutations.Subjects: We report a patient of HBSL with compound heterozygous mutations in DARS1. To study the potential genetic variations of the patient, targeted next-generation sequencing, whole-exome sequencing, and Sanger sequencing were used. We reviewed the clinical and radiological features of the patient. The literature was thoroughly evaluated.Results: The patient suffered from developmental regression associated with lower limbs spasticity, developmental delay, and paralysis of the lower limbs since childhood. Decreased T1 and increased T2 signals were observed on the bilateral basal, centrum ovale, frontal lobe, parietal lobe, and ganglia in cervical cord magnetic resonance imaging (MRI). The patient had two compound heterozygous mutations (NM_001349:c.1363T &gt; C and NM_001349:c.821C &gt; G) in the DARS1 gene.Conclusion: Two mutations in DARS1 were found to be associated with HBSL, one of them being reported for the first time. These findings can be valuable for diagnosing and providing genetic counseling to HBSL patients in the future.
Comprehensive Analysis of the Correlation Between Pyroptosis-Related LncRNAs and Tumor Microenvironment, Prognosis, and Immune Infiltration in Hepatocellular Carcinoma
Authors: Qu Guangzhen , Wang Dong , Xu Weiyu , Guo Wei 
Published: April 2022

Background: Accumulating evidence shows that pyroptosis plays a crucial role in hepatocellular carcinoma (HCC). However, the relationship between pyroptosis-related long non-coding RNAs (lncRNAs) and HCC tumor characteristics remains enigmatic. We aimed to explore the predictive effect of pyroptosis-related lncRNAs (PRLs) in the prognosis of HCC.Methods: We comprehensively analyzed the role of the PRLs in the tumor microenvironment and HCC prognosis by integrating genomic data from patients of HCC. Consensus clustering analysis of PRLs was applied to identify HCC subtypes. A prognostic model was then established with a training cohort from The Cancer Genome Atlas (TCGA) using univariate and least absolute shrinkage and selection operator (LASSO) Cox regression analysis. Further, we evaluated the accuracy of this predictive model using a validation set. We predicted IC50s of commonly used chemotherapeutic and targeted drugs through the R package pRRophetic.Results: Based on pyroptosis-related lncRNAs, a prognostic risk signature composed of seven PRLs (MKLN1AS, AL031985.3, SNHG4, GHRLOS, AC005479.2, AC099850.4, and AC026412.3) was established. For long-term prognosis of HCC patients, our model shows excellent accuracy to forecast overall survival of HCC individuals both in training set and testing set. We found a significant correlation between clinical features and the risk score. Patients in the high-risk group had tumor characteristics associated with progression such as aggressive pathological grade and stage. Besides that, gene set enrichment analysis (GSEA) showed that cell cycle and focal adhesion were significantly enriched in the high-risk group.Conclusion: The association of the risk model constituted by these seven pyroptosis-related lncRNAs with clinical prognosis, tumor microenvironment, chemotherapy and small molecule drugs was evaluated. Our study provides strong evidence for individualized prediction of prognosis, shedding light on immunotherapy in HCC patients.
Altered Expressions of NF1 and NF1-Related microRNAs as Biomarkers in the Diagnosis of Undifferentiated Pleomorphic Sarcoma
Authors: Zhang Peng , Huang Lingling , Ma Pengwei , Niu Xiaoying 
Published: April 2022

Objective: Undifferentiated pleomorphic sarcoma (UPS) is a highly malignant, aggressive, and pleomorphic subtype of soft tissue sarcoma in adults. However, UPS is difficult to be diagnosed due to the lack of specific morphological and immunophenotypic features. Here, we aimed to identify new biomarkers for the diagnosis of UPS.Methods: The mRNA and protein expression of neurofibromin 1 (NF1) in 68 pairs of UPS and adjacent normal tissues were detected by qRT-PCR and immunohistochemistry, and the correlation between the NF1 protein expression and clinicopathological characteristics was analyzed. Then, differentially expressed microRNAs (DE miRNAs) were identified between the UPS tumor tissue and matched adjacent normal tissue using Hisep sequencing, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG). The DE miRNAs of the regulating NF1 gene were also identified using the TargetScan and miRanda databases and validated by qRT-PCR.Results: Compared with the adjacent normal tissue, both mRNA and protein expressions of NF1 in the UPS tumor tissue were significantly decreased, and the positive rate of NF1 protein was associated with the tumor size, metastasis, and recurrence. A total of 125 known DE miRNAs were identified from the screened miRNAs based on | log2(Fold Change) ≥5 and p-value &lt; 0.05 (A total of 82 upregulated and 43 downregulated DE miRNAs in the UPS tissue). Target genes regulated by the DE miRNAs were enriched in pathways of metabolisms, RNA degradation, PI3K-Akt, and Rap1 pathway. In total, 11 miRNAs which were predicted to regulate the NF1 gene were screened. After verification, the relative expressions of hsa-miR-199a-3p and hsa-miR-34a-5p were increased and decreased in the UPS tumor tissue compared with those in the adjacent normal tissue, respectively.Conclusion: NF1 and NF1-related microRNAs including hsa-miR-199a-3p and hsa-miR-34a-5p may be novel biomarkers in the diagnosis of undifferentiated pleomorphic sarcoma (UPS).
Mutations in Growth-Related Genes Induced by EMS Treatment in Scallops
Authors: Wang Caihui , Liu Bo , Chen Min , Ning Junhao , Lu Xia , Wang Chunde 
Published: April 2022

Background: The goal of genetic breeding is to select variants with mutations that are related to expected traits, such as fast growth. Artificial induction has been widely used to obtain strains with more mutations for further selection. Ethylmethylsulfone (EMS) is one of the most commonly used chemical mutagens in plant and microorganism breeding. However, the application of EMS mutagenesis in shellfish has not been reported. The aim of this study is to evaluate the potential use of EMS as a mutagen in scallop breeding, especially in characterization of mutations in growth-related genes.Results: Our results indicated that hatching of about 50% of fertilized eggs was blocked by treatment with 20 mM EMS for 3 h and the resulted larvae developed normally into adult stages. We then evaluated the mutagenic effects of EMS by sequencing the genomes of 4 adult scallops from the control group and 12 from the treatment group at 8 months after fertilization. On average, after removing shared types of mutations, there were 1,151,380 ± 258,188 SNPs (Single Nucleotide Polymorphisms) and 229,256 ± 51,714 InDels (insertion-deletion) in each animal in the EMS treatment group, while there were only134841 ± 10,115 SNPs and 42,605 ± 5,136 InDels in the control group. The average mutation rate in the genome of the EMS treatment group (0.0137 ± 0.0013%) was about 9 times that of the control group (0.0015 ± 0.0002%). GO (Gene Ontology) annotation and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses revealed that mutations induced by EMS occurred evenly in most biological processes, cellular components and functions, as well in most pathways. However, significant lower percentage of mutations were found in the exonic region, in non-synonymous or Stopgain/Stoploss SNPs and in coding domains, suggesting apparent DNA repair or selection during grow-out stage. Analyses of the growth-related genes with mutations indicated that mutations in MFS (Major Facilitator Superfamily) and Tubulin were only found in the large-sized group (Five largest scallops: Treated-1, Treated-2, Treated-3, Treated-4, and Treated-5) and Homeobox and Socs (Suppressor of cytokine signaling) only in the small group (Two smallest scallops: Treated-11 and Treated-12). These results suggested that these genes may be involved in the regulation of growth in these animals, although further verification is certainly warranted.Conclusion: Treatment of fertilized eggs with 20 mM EMS for 3 h induced 9 times more mutations in scallop genomes. We found that mutations in MFS and Tubulin may be related to fast growth in the large-sized group and those mutations in Homeobox and SOCs may be involved in the slow growth in the small-sized scallops. EMS can be used to accelerate selection of economically important traits in molluscs.
HIV-1-Transmitted Drug Resistance and Transmission Clusters in Newly Diagnosed Patients in Portugal Between 2014 and 2019
Authors: Pingarilho Marta , Pimentel Victor , Miranda Mafalda N. S. , Silva Ana Rita , Diniz António , Ascenção Bianca Branco , Piñeiro Carmela , Koch Carmo , Rodrigues Catarina , Caldas Cátia , Morais Célia , Faria Domitília , da Silva Elisabete Gomes , Teófilo Eugénio , Monteiro Fátima , Roxo Fausto , Maltez Fernando , Rodrigues Fernando , Gaião Guilhermina , Ramos Helena , Costa Inês , Germano Isabel , Simões Joana , Oliveira Joaquim , Ferreira José , Poças José , da Cunha José Saraiva , Soares Jorge , Henriques Júlia , Mansinho Kamal , Pedro Liliana , Aleixo Maria João , Gonçalves Maria João , Manata Maria José , Mouro Margarida , Serrado Margarida , Caixeiro Micaela , Marques Nuno , Costa Olga , Pacheco Patrícia , Proença Paula , Rodrigues Paulo , Pinho Raquel , Tavares Raquel , de Abreu Ricardo Correia , Côrte-Real Rita , Serrão Rosário , Castro Rui Sarmento e , Nunes Sofia , Faria Telo , Baptista Teresa , Martins Maria Rosário O. , Gomes Perpétua , Mendão Luís , Simões Daniel , Abecasis Ana 
Published: April 2022

ObjectiveTo describe and analyze transmitted drug resistance (TDR) between 2014 and 2019 in newly infected patients with HIV-1 in Portugal and to characterize its transmission networks.MethodsClinical, socioepidemiological, and risk behavior data were collected from 820 newly diagnosed patients in Portugal between September 2014 and December 2019. The sequences obtained from drug resistance testing were used for subtyping, TDR determination, and transmission cluster (TC) analyses.ResultsIn Portugal, the overall prevalence of TDR between 2014 and 2019 was 11.0%. TDR presented a decreasing trend from 16.7% in 2014 to 9.2% in 2016 (pfor–trend = 0.114). Multivariate analysis indicated that TDR was significantly associated with transmission route (MSM presented a lower probability of presenting TDR when compared to heterosexual contact) and with subtype (subtype C presented significantly more TDR when compared to subtype B). TC analysis corroborated that the heterosexual risk group presented a higher proportion of TDR in TCs when compared to MSMs. Among subtype A1, TDR reached 16.6% in heterosexuals, followed by 14.2% in patients infected with subtype B and 9.4% in patients infected with subtype G.ConclusionOur molecular epidemiology approach indicates that the HIV-1 epidemic in Portugal is changing among risk group populations, with heterosexuals showing increasing levels of HIV-1 transmission and TDR. Prevention measures for this subpopulation should be reinforced.
A Cross-Sectional Study of Breast Cancer Surgery and the Cost Based on Data From 77 Chinese 3A Hospitals in 2015
Authors: Zhao Rong , Jin Meng , Gao Jinnan , Zhang Linhui , Tao Liyuan , Bao Xiaoyuan 
Published: April 2022

PurposesWe aimed to clarify the real-world status of breast cancer surgery and the cost in China.MethodsThis cross-sectional survey relied on data obtained from the hospitalization summary reports (HSRs) in 77 top-ranked (grade 3A) hospitals in China to analyze breast cancer patients who underwent surgery between January 2015 and December 2015. The surgery and cost were mainly evaluated.ResultsOverall, 31,900 breast cancer patients underwent surgeries in 77 hospitals. The mean age in our study was 51.5 years (SD, 11.7 years). The primary types of surgical procedures were mastectomy (n = 24,629, 77.2%) and breast-conserving surgery (6,210, 19.5%). The rate of mastectomy was the highest at age band 50–65 years (n = 10,861, 82.1%) and in non-first-tier cities (n = 7,651, 88.4%) as well as in Northeast China (n = 3,107, 93.2%). The rate of breast-conserving surgery was less than 10% in non-first-tier cities (9.8%), Southwest China (6.1%), and Northeast China (5.8%). The median cost was $3,352.4 (interquartile range (IQR), $2,492.6–4,588.0). Mastectomy cost was significantly higher than breast-conserving surgery cost in both different city tiers and regional distribution except Northeast China (p &lt; 0.001).ConclusionsThis study demonstrated that the main breast cancer surgery in Chinese 3A hospitals was mastectomy and that the cost varied both across and within geographic regions and city tiers. This information helps describe the real-world status of breast surgery and the cost in China.