bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal Stem Cells Modified with Angiotensin Converting Enzyme 2 are Superior in Ameliorating Inflammation and Oxidative Stress in Diabetic Nephropathy
Authors: Lv Shasha , Liu Shanshan , Sun Yun , Cheng Jing , Dou Fulin , Xu Qiaoying , Wang Yinghui , Liu Qingzhen , Liu Gang 
Published: April 2022

Abstract
Background
The purpose of this study is to detect the effect of angiotensin converting enzyme 2 (ACE2)-modified mesenchymal stem cells (MSCs) on inflammation and oxidative stress in diabetic nephropathy (DN) and investigate the underlying mechanism.
Methods
MSCs transduced with a lentiviral vector overexpressing ACE2 (MSCs-ACE2) were transplanted in streptozotocin (STZ)-induced diabetic rats and cocultured with glomerular mesangial cells (GMCs) following Ang II stimulation in vitro. Biochemical parameters were measured. Extracellular matrix (ECM) proteins, inflammation, oxidative stress indicators and renin-angiotensin system (RAS) elements were detected. To explore possible molecular mechanisms, the phosphorylation levels of p38 MAPK, and IκB-α in GMCs were measured by Western blotting.
Results
MSCs-ACE2 treatment significantly decreased urinary albumin excretion and ECM deposition in the mesangium, downregulated the expression of inflammatory cytokines, decreased the production of ROS and MDA, increased the activity of SOD and the ratio of GSH to GSSG than MSCs treatment alone in vivo. In vitro, after MSC-ACE2 treatment, ACE2 expression in GMCs was significantly upregulated, and its product Ang1-7 correspondingly increased, the indicators of inflammation and oxidative stress were ameliorated by downregulation of Ang II expression in GMCs; moreover, the phosphorylation levels of p38 MAPK and IκB-α protein were significantly downregulated.
Conclusions
Based on these results, we believed that MSCs modified with ACE2 therapy exhibited additional benefits on the progression of DN by inhibiting renal RAS activation and reducing inflammation and oxidative stress in vivo and in vitro. The possible mechanisms were potentially inhibition of p38 MAPK and NF-kB pathway activation.
Who may address older adults’ subjective memory complaints better as a community health worker? A cross sectional study
Authors: Gong De , Yang Tiantian , Peng Yan , Yang Yanni 
Published: April 2022

Abstract
Background: Dementia has been a worldwide health issue, with an increasing focus on primary prevention. It is necessary for community health workers to detect early symptoms of dementia and deal with them correctly. However, few studies have explored how community health workers respond to older people with subjective memory complaints (SMC), and especially whether this response is appropriate.Objectives: This study aimed to evaluate the features of community health workers, based on their different types of responses to SMCs from older adults and explore the related influencing factors.Design: A cross-sectional studySettings: Online questionnaires were presented to doctors and nurses in four primary health care institutions from April to July 2020.Participants: Community health workers, including doctors and nurses, were recruited in this study.Methods: Questionnaires were provided through We-Chat. Participants were divided into three Types: Refer to specialists (Type A), perform cognitive screening (Type B), and ignore or change the subject (Type C). Thereafter, self-made measures were used to evaluate the knowledge and self-efficacy of community health workers. The differences in general characteristics, as well as the knowledge and self-efficacy scores for dementia prevention among the three Types, were analyzed.Results: This study’s participants included 268 community health workers: 127 were Type A, 122 were Type B, and 19 were Type C. Participants with different occupations, contact history with dementia patients, and history of receiving dementia-related training, as well as different levels of knowledge and self-efficacy, may have had different responses to individuals’ reported cognitive problems (all p< 0.05). In addition, the contact history with dementia patients (Odds ratio, 95%CI=2.090, 1.131–3.861) and self-efficacy scores (Odds ratio, 95%CI=1.055, 1.012–1.100) could be positive factors influencing community health workers’ choices to perform cognitive screening. Conclusion: This study explored different responses of community health workers to the SMCs of older adults. It found that participants who preferred to perform cognitive screening had higher related knowledge and self-efficacy levels. Further, participants’ contact history with dementia patients and self-efficacy scores may positively influence their choices to perform cognitive screening. These findings could be helpful to carry out targeted education and training to improve the willingness and ability of community health workers to prevent dementia, so as to strengthen the primary prevention of dementia.
Deciphering the Roles of CircRNAs on Hepatic Metastases in Early Tumor Stage Colorectal Cancer Patients
Authors: Huang Maosen , Cheng Linyao , Ru Haiming , Wei Chunyin , Mo Xianwei , Yan Linhai 
Published: April 2022

Abstract
Objective: Circular RNAs (circRNAs), a specific type of non-coding RNAs, could regulate tumorigenesis and metastasis of various cancers though acting as competing endogenous RNA (ceRNAs).Methods: Performing next generation sequencing (NGS) and bioinformatics methods, the profiles and the intricate roles of circRNAs were clarified in early stage colorectal cancer patients with hepatic metastases.Results: CircRNAs were predicted to be involved in CRC hepatic metastasis complex processes, such as p53 binding and ErbB excision repair. Intriguingly, we detected 19 significantly differentially expressed among CRC patients with or without hepatic metastases and found circFARSA was significantly upregulated. Besides, circFARSA was found to be potential ceRNAs and a triple regulatory network of circFARSA/hsa-miR-503-5p/CCND2 was set up, which describes the possible mechanisms of CRC hepatic metastasis.Conclusions: Our findings demonstrated that circFARSA acts as a promising biomarker in metastatic colorectal cancer (mCRC). The study provided a new perspective for targeting circFARSA in mCRC therapeutic treatment.
Circ_0000732 promotes breast cancer cell proliferation, metastasis and stemness by targeting COL11A1 through the regulation of miR-1253
Authors: Liu Xin , Ming Wenjian , Wu Yuanbo , Wang Fang 
Published: April 2022

Abstract
Background: Many studies have shown that abnormal circular RNA (circRNA) expression is associated with the malignant progression of breast cancer (BC), but the role of circ_0000732 in BC progression remains unclear.Methods: The expression of circ_0000732, microRNA (miR)-1253 and collagen XI alpha 1 (COL11A1) was measured by quantitative real-time PCR. Cell proliferation, migration, invasion and stemness were assessed by cell counting kit 8 assay, Edu assay, transwell assay and sphere formation assay. Western blot analysis was used to determine protein expression. Dual-luciferase reporter assay was performed to assess the interaction between miR-1253 and circ_0000732 or COL11A1. The effect of circ_0000732 on BC tumor growth was confirmed by animal experiments.Results: Circ_0000732 was overexpressed in BC tissues and cells, and its knockdown suppressed BC cell proliferation, metastasis and stemness. MiR-1253 could be sponged by circ_0000732, and anti-miR-1253 overturned the effects of circ_0000732 knockdown on BC cell progression. COL11A1 was targeted by miR-1253, and miR-1253 inhibited BC cell progression by targeting COL11A1. Circ_0000732 could sponge miR-1253 to upregulate COL11A1. Also, interference of circ_0000732 decreased BC tumor growth by miR-1253/COL11A1 pathway. Conclusion: Our data showed that circ_0000732 might be a potential target for BC treatment, which could enhance BC malignant phenotype through miR-1253/COL11A1 axis.
All-Dielectric Metasurface Lenses for Achromatic Imaging Applications
Authors: Li Menghan , Liu Muhan , Chen Yuxuan , Hu Zheng-Da , Wu Jingjing , Wang Jicheng 
Published: April 2022

Abstract
Metasurface can use artificial microstructures to manipulate electromagnetic waves more accurately and flexibly. All-dielectric metalens have a wide range of materials and low cost so it has a wide application prospect. Herein, we propose a all-dielectric achromatic metalens that can operate over a broadband of wavelengths in the visible region. It controls the wavefront of light through the Pancharatnam-Berry phase and propagation phase to eliminate the chromatic aberration. Meanwhile, we also use Gerchberg-Saxton algorithm and its improved algorithm to iterate over multiple design wavelengths and obtain holographic phases suitable for broadband. Thus, both the metalenses and holographic metasurfaces can achieve achromatic broadband in the visible light range, which provides a new method for the development of meta-optical imaging devices.
Identification and annotation of peptide allergens in Prunus dulcis
Authors: Singh Arshwinder , Upadhyay Atul Kumar 
Published: April 2022

Abstract
Approximately, 10% of the world population is facing the challenge of food allergy in direct or indirect way. In this study, a genome-wide identification and annotation of the novel putative allergen from Almond is performed. Initially, the whole proteome of Almond (31,000 proteins) was scanned by Allergenonline, a publically available database of already reported allergens from different sources. The detailed analysis suggests that there are 430 putative allergens which reduced to 45 on motif-based screening using AllFam database. These predicted allergens are annotated for their function by using PFAM, GO databases and orthology analysis. To validate our prediction, we have used structural insights of allergen and antibody interactions for one of the predicted putative allergen protein, homologous to Pru ar 3.0101allergen from Apricot. The structure of putative allergen was modeled and molecular docking studies were performed against the antibody. The best docked conformation was subjected to molecular simulation studies to confirm the stable binding of these two molecules. This detailed analysis suggests that the identified allergen will show cross reactivity similar to Pru ar 3.0101 allergen from Apricot. This is one of the first report of identifying and annotating the homologous of Pru ar 3.0101 allergen in Almond.
Influence of Vesicular-arbuscular mycorrhiza fungi species on growth and water status of Para rubber seedlings under well-watered and drought-stressed conditions
Authors: OGBEBOR Nicholas Obehi , Adekunle Adefunke Temitayo , Ohiocheoya Benjamin 
Published: April 2022

Abstract
Vesicular-arbuscular mycorrhizal (VAM) fungi spores from soil samples collected from mature rubber plantations were isolated and multiplied in pot cultures with Zea mays. The VAM fungi culture where designated N4 and OP9 and GD (Glomus deserticola). The plants were laid out in a completely randomized format. Drought stress was induced for four months before the start of the experiment. Leaf water potential (Ψ), Leaf Relative Water Content (RWC) and Water deficit were evaluated. The RWC and water deficit did not show any significant different among the VAM fungi evaluated (p < 0.05). Stressed seedlings of RRIC 45 in the VAM treatments OP9/NS and GD/NS in the drought regime recorded RWC and water deficit that were comparable to their replicates from the wet regimes. More replicates from GT 1 seedlings recorded higher Ψ compared to those from the wet regime. Similarly, Higher RWC and water deficit was exhibited by more replicates of GT 1 seedlings from the drought regime. The interdependent of VAM fungi N4 and roots of seedlings of RRIC 45 resulted in the highest drought tolerance (Ψ and RWC) clone. Similarly, interdependent of VAM fungi with roots of seedlings of GT 1 demonstrated that VAM fungi GD and OP9 are better VAM fungi for drought tolerance GT 1 clone compared. The improvement of replicates under drought regime is attributed to the fact that the VAM fungi a vital role. This study recommends the use of VAM fungi as bio-inoculants in sustainable rubber trees production in marginal rubber growing regions.
PDIA3P1 Promotes Temozolomide Resistance in Glioblastoma by Inhibiting C/EBPβ Degradation to Facilitate Proneural-to- Mesenchymal Transition
Authors: Gao Zijie , Xu Jianye , Fan Yang , Qi Yanhua , Wang Shaobo , Zhao Shulin , Guo Xing , Xue Hao , Deng Lin , Zhao Rongrong , Sun Chong , Zhang Ping , Li Gang 
Published: April 2022

Abstract
BackgroundResistance to temozolomide (TMZ) is a major obstacle to prevention of recurrence after surgery for glioblastoma (GBM). Although long noncoding RNAs (lncRNAs) play wide roles in GBM, the lncRNAs that regulate TMZ resistance have not been elucidated clearly. This study aims to identify lncRNAs that may affect TMZ treatment sensitivity and to explore novel therapeutic strategies to overcome TMZ resistance in GBM.MethodsThe lncRNAs associated with TMZ resistance were explored based on Cancer Cell Line Encyclopedia (CCLE) dataset and Genomics of Drug Sensitivity in Cancer (GDSC) dataset. Quantitative real-time PCR (qRT-PRC) was used to detect the expression of PDIA3P1 in TMZ-resistant and TMZ-sensitive GBM cell lines. Both gain-of-function and loss-of-function studies were used to assess the effects of PDIA3P1 on TMZ resistance through in vitro and in vivo assays. Glioma Stem Cells (GSCs) were used to determine the effect of PDIA3P1 on GBM subtype. The hypothesis that PDIA3P1 promotes proneural-to-mesenchymal transition (PMT) was established by bioinformatics analysis and functional experiments. RNA pull-down and RNA immunoprecipitation (RIP) assays were performed to examine the interaction between PDIA3P1 and C/EBPβ. The post-translational modification mechanism of C/EBPβ was verified using ubiquitination and co-immunoprecipitation (co-IP) experiments. The CompuSyn was leveraged to calculate the combination index (CI) and the antitumor effect of TMZ with Neflamapimod (NEF) was validated both in vitro and in vivo.ResultsWe identified a lncRNA, PDIA3P1, which was upregulated in TMZ-resistant GBM cell lines. Overexpression of PDIA3P1 promoted the acquisition of TMZ resistance, whereas knockdown of PDIA3P1 restored TMZ sensitivity. PDIA3P1 was upregulated in MES-GBM, promoted PMT progression in GSCs, and caused GBMs to be more resistant to TMZ treatment. Mechanistically, PDIA3P1 disrupted C/EBPβ-MDM2 complex and stabilized C/EBPβ protein by preventing it from MDM2-mediated ubiquitination. The expression of PDIA3P1 was up-regulated in a time- and a concentration-dependent manner after TMZ treatment. The TMZ-induced upregulation of PDIA3P1 was mediated by p38α-MAPK signaling pathway. NEF is a small molecule drug that specifically targets p38α with excellent blood-brain barrier (BBB) permeability. NEF could block TMZ-responsive PDIA3P1 upregulation and produced synergistic effects with TMZ at specific concentrations. The combination of TMZ and NEF exhibited excellent synergistic antitumor effects in vitro and in vivo.ConclusionsPDIA3P1 promoted PMT through stabilization of C/EBPβ, thereby reducing the sensitivity of GBM cells to TMZ treatment. NEF could inhibit TMZ-responsive PDIA3P1 upregulation and NEF combined with TMZ could provide better anti-tumor effects.
Effect Of Thrombolytic Therapy On High Neutrophil-to-Lymphocyte Ratio In Predicting Stroke-Associated Pneumonia After Acute Ischemic Stroke
Authors: Chen Liu-Zhu , Xia Hu-Wei , Zhan Lu-Qian , Lin Yi-Si , Xu Xue-Qian , He Jin-Cai , Luan Xiao-Qian 
Published: April 2022

Abstract
Background and Purpose
In patients with acute ischemic stroke (AIS) undergoing intravenous thrombolysis (IVT), there are few studies assessing the predictive effect of blood cell inflammatory markers on stroke-associated pneumonia (SAP), short-term and long-term prognosis. In this study, we evaluated whether the neutrophil-to-lymphocyte ratio (NLR) can predict SAP and functional prognosis in patients receiving IVT.
Methods
We assessed 388 patients undergoing IVT for acute ischemic stroke. Blood parameters were sampled before thrombolysis (within 30 mins after admission) and within 12–24 h after thrombolysis. The primary outcome measure was the occurrence of SAP as well as the prognosis after IVT. Multivariate logistic regression analysis was performed to analyze the association between NLR and the occurrence of SAP. We also used receiver operating characteristic curve analysis to assess the discriminative ability of NLR measured at different times in predicting SAP and prognosis. Further exploration of the correlation between NLR and baseline variables was conducted using Pearson correlation and multivariate linear regression analysis.
Results
Among the total patients, SAP occurred in 60 (15%) patients. Multivariate analysis showed that NLR was significantly associated with SAP (NLR before IVT: aOR = 1.288; 95%CI = 1.123–1.476; p < 0.001; NLR after IVT (aOR = 1.113, 95%CI = 1.007–1.23; p = 0.036). The receiver operating characteristic curve showed that the predictive ability of NLR after IVT was better than NLR before IVT (area under the curve [AUC] of NLR after IVT: 0.80, 95%CI = 0.74–0.86; p < 0.001; NLR before IVT: 0.72, 95%CI = 0.66–0.80; p < 0.001), not only in predicting the occurrence of SAP but also in predicting short-term functional outcomes and one-year mortality. Furthermore, NLR after IVT was significantly correlated with the initial NIHSS score (p < 0.001) and early neurological outcomes after IVT (p < 0.001).
Conclusions
We demonstrated that increased NLR has a significant predictive effect on the occurrence of SAP and can be used to predict functional dependence and one-year mortality. NLR measured within 12–24 h after IVT had better predictive validity than that measured before IVT.
Differential effects of positive and negative expectations on depressive symptoms
Authors: Kube Tobias , Herzog Philipp 
Published: April 2022

Abstract
Background
Recent research suggests that dysfunctional expectations are a particularly important subtype of cognitions in depression. However, it is unclear whether depressive symptoms are primarily related to the presence of negative expectations, the absence of positive expectations, or both.
Methods
Using hierarchical linear regression and mediation analyses, the present study examined the predictive value of positive situation-specific expectations, negative situation-specific expectations, and other cognitive factors on depressive symptoms eight weeks later in a non-clinical sample (N = 157).
Results
Cross-sectionally, depressive symptoms were more strongly associated with the presence of negative expectations than with the absence of positive expectations. In the regression analysis, negative expectations rather than positive expectations predicted depressive symptoms eight weeks later, but only if baseline depressive symptoms were not considered. Negative but not positive situation-specific expectations mediated the effects of both dispositional optimism and dysfunctional attitudes on depressive symptoms eight weeks later.
Conclusions
In non-clinical samples, the presence of negative expectations rather than the absence of positive expectations may be associated with depressive symptoms. The current results also provide further evidence for a cognitive mediational model, suggesting that the effects of dysfunctional global cognitions on depressive symptoms are mediated through dysfunctional situation-specific expectations.
Using NSQIP data to determine when drains can be safely remove after pancreaticoduodenectomy
Authors: Nicolais Laura , BS Abdimajid Mohamed , Fitzgerald Timothy L. 
Published: April 2022

Abstract
Purpose: Prior retrospective studies demonstrate the need for drainage after pancreatoduodenectomy. Early drain removal is a key component of most ERAS pathways; however, little consensus on timing and cutoff for amylase analysis exists. This study aimed to identify the ideal combination of postoperative day and drain amylase level for safe drain removal.Methods: This retrospective cohort study used the NSQIP database to study outcomes of pancreatoduodenectomy patients. Results: 7,583 patients underwent pancreatoduodenectomy with drain placement. 1,458 (19%) had a clinically relavent postoperative pancreatic fistula (CR-POPF). Of those, 7% had their highest drain amylase on day 1, compared to 29% and 64% on day 3-5 and 5, respectively (p < 0.001). An amylase level >300 U/L corresponded to a 46% chance of CR-POPF compared to 4.9% with amylase <300 U/L (p < 0.001). Drain amylase >5000 U/L, corresponded to 71.2% chance of CR-POPF compared to 11.2% with amylase <5000 U/L (p < 0.001). Compared to >5000 U/L, an amylase cutoff of >300 U/L had a lower specificity on day 1 (93%, CI: 0.92, 0.93) and further decreases on postoperative day 3-5 (81%, CI: 0.79-0.98). The sensitivity of a cutoff >300 U/L was superior: 24% (CI: 0.19-0.29) on postoperative day 1 but increased significantly to 72% (CI: 0.67-0.76) on postoperative days 3-5. Conclusions: Current recommendations utilizing 5000 U/L will miss 6% of patients with CR-POPF. We recommend checking drain amylase on postoperative day three and to use 300 U/L as a cutoff for early drain removal protocols.
Displacement mitigation mechanism and parameters sensitivity analysis of steel moment frame retrofitted by self-centering braces
Authors: Lu Xiao , Xu Longhe 
Published: April 2022

Abstract
The steel moment frame (SMF) designed according to modern codes can prevent collapse and protect life safety, but may still experience excessive damage and residual displacement, resulting in high repair costs and long recovery time. The self-centering brace (SCB) is one of the most efficiency components to reduce the story drift and residual displacement of SMF. Although the mechanism of SCB reducing residual displacement has been fully studied, the mechanism of reducing story drift has not been well explained. Based on the equivalent single degree of freedom (SDOF) model, 24 analysis models of SMF, SMF retrofit by buckling restrained brace (BRBF), and SMF retrofit by self-centering brace (SCBF) with different fundamental periods are established in this paper. The seismic displacement mitigation mechanism of SCB is first comparatively studied using nonlinear time history analysis. Following this, 176 equivalent SDOF models representing a series of SCBFs with different key parameters (initial stiffness, post-activation stiffness coefficient and activation strength of SCB system) are established, and the influence of the key parameters of SCB on seismic responses of SCBF is discussed. Reasonable parameter value ranges are then proposed. Results show that the self-centering capacity of SCB can not only successfully reduce the residual displacement of the structure, but also effectively reduce the maximum displacement. The optimal value of the initial stiffness of the SCB is 0.6 ~ 1.2 times the initial stiffness of the SMF, the post-activation stiffness coefficient of the SCB should not exceed 0.1, and the optimal value of the activation strength of the SCB is 0.6 ~ 1.2 times the yield strength of the SMF.