bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Anesthetic Agents on Blood Brain Barrier Integrity: A Systematic Review
Authors: Rizk Abanoub Aziz , Plitman Eric , Senthil Pooja , Venkatraghavan Lashmi , Chowdhury Tumul 
Published: November 2022

ABSTRACT:Background:The blood brain barrier (BBB) is a highly selective permeable barrier that separates the blood and the central nervous system. Anesthesia is an integral part of surgery, and there is little known about the impact of anesthetics on the BBB. Therefore, it is imperative to explore reversible or modifiable variables such as anesthetic agents that influence BBB integrity. We aimed to synthesize the literature pertaining to the various effects of anesthetics on the BBB.Methods:MEDLINE, Embase, and Cochrane were searched from inception up to September 2022.Results:A total of 14 articles met inclusion into the review. The articles included nine randomized control studies (64.3%) and five quasi-experimental studies (35.7%). Twelve studies used volatile anesthetics, one study used fentanyl intravenously, and one study used pentobarbital or ketamine intraperitoneally. BBB structural deficits following the administration of an anesthetic agent included ultrastructural deficits, decreases in tight junctions, and decreases in BBB components. BBB functional deficits included permeability increases following exposure to volatile anesthetics. However, two studies found decreased permeability after fentanyl, pentobarbital, or ketamine exposure. Moreover, the impact of anesthetics on the BBB seems to be related to the duration of exposure. Notably, study findings also suggest that changes following anesthetic exposure demonstrate some reversibility over the short-term.Conclusion:Overall, our systematic review highlights interesting findings pertaining to the impact of anesthetic agents on BBB integrity in previously healthy models. These findings and mechanisms should inspire future work to aid practitioners and healthcare teams potentially better care for patients.
Confucius and the varifocal stance
Authors: Lai Karyn , Stapleton Mog 
Published: November 2022

AbstractWe put the bifocal stance theory (BST) into dialogue with the Confucian approach to ritual. The aim of the commentary is two-fold: To draw on BST to provide an explanatory framework for a Confucian approach to social learning and, while doing so, to show how Chinese (Confucian) philosophy can contribute to debates in cultural evolution.
From flows towards updates: Security regimes and changing technologies for financial surveillance
Authors: Westermeier Carola 
Published: November 2022

Abstract‘Follow the money’ is currently the central principle of international financial security, although money itself is probably one of the most unlikely objects to make traceable. Two recent scandals around a security unit and the payment processor Wirecard show how existing systems of financial surveillance that seek to capture ‘flows’ of money for security purposes are either enabled or frustrated. While this current regime of financial surveillance adheres to demanding the free flow of money through financial infrastructures and various actors and intermediaries, new digital currencies build on a set type of ledger(s) in which money is stored as data. Hence, what we understand as money does not ‘flow’, but is rather updated. This change in the underlying infrastructure means that traceability does not need to be enacted; it is an intrinsic feature of digital currencies. With new central bank digital currencies (CBDC), the regime of financial security thus changes from the monitoring of financial flows and flagging of (potentially) illicit transactions towards the storage of financial data in (de)centralised ledgers. This form of transactional governance is engendered by shifting geopolitical agendas that increasingly rely on fractured instead of globalised financial infrastructures, thus making CBDCs themselves subject to security efforts.
Expansion and Intensification of the North American Monsoon During the Pliocene
Authors: Bhattacharya Tripti , Feng Ran , Tierney Jessica E. , Rubbelke Claire , Burls Natalie , Knapp Scott , Fu Minmin 
Published: November 2022

AbstractSouthwestern North America (SWNA), like many subtropical regions, is predicted to become drier in response to anthropogenic warming. However, during the Pliocene, when carbon dioxide was above pre‐industrial levels, multiple lines of evidence suggest that SWNA was much wetter. While existing explanations for a wet Pliocene invoke increases in winter rain, recent modeling studies hypothesize that summer rain may have also played an important role. Here, we present the first direct evidence for an intensified mid‐Pliocene monsoon in SWNA using leaf wax hydrogen isotopes. These new records provide evidence that the mid‐Pliocene featured an intensified and expanded North American Monsoon. Using proxies and isotope‐enabled model simulations, we show that monsoon intensification is linked to amplified warming on the southern California margin relative to the tropical Pacific. This mechanism has clear relevance for understanding present‐day monsoon variations, since we show that intervals of amplified subtropical warming on the California margin, as are seen during modern California margin heat waves, are associated with a stronger monsoon. Because marine heat waves are predicted to increase in frequency, the future may bring intervals of “Pliocene‐like” rainfall that co‐exist with intensifying megadrought in SWNA, with implications for ecosystems, human infrastructure, and water resources.
Density Staircases Are Disappearing in the Canada Basin of the Arctic Ocean
Authors: Ménesguen Claire , Lique Camille , Caspar‐Cohen Zoé 
Published: November 2022

AbstractIn the Canada Basin of the Arctic Ocean, warm and salty Atlantic‐origin Water (AW) lies in the intermediate layer (250–800 m) below a colder and fresher surface layer. It results in a depth range where vertical thermohaline gradients are propitious to double‐diffusion. Indeed, thermohaline staircases are commonly observed and associated with double‐diffusive processes. Using observations from the Beaufort Gyre Exploration Project large database and Ice‐Tethered Profilers, we document the presence of density staircases in the 300–700 m depth range with a striking strong spatial and temporal coherence. However, since 2007, a progressive smoothing of these staircases has occurred, beginning from the western half of the basin. Quantifying this evolution, we find that a general pattern is a clear evolution over time from numerous thick steps (≃40 m) with sharp interfaces to fewer and thinner steps (≃30 m) with smoother interfaces. After 2014, marked density staircases have almost disappeared in most of the Canada Basin. The vanishing of staircases occurs over a few years and coincides with modifications of the large scale circulation and thermohaline large scale horizontal gradients. As the small scale thermohaline structures are thought to play an important role for the vertical and horizontal exchanges of heat within the Canada Basin, the disappearance of the steps may impact the heat distribution at depth, with potential consequences for the evolution of the sea ice cover.
Practice Patterns for Addressing Developmental-Behavioral Concerns in Sickle Cell Specialty Care
Authors: Schlenz Alyssa M. , Phillips Shannon , Mueller Martina , Kanter Julie 
Published: November 2022

Objective: Children with sickle cell disease (SCD) are at elevated risk for neurodevelopmental and behavioral disorders. This article describes developmental-behavioral practice patterns among sites who were part of the Dissemination and Implementation of Stroke Prevention Looking at the Care Environment (DISPLACE) consortium in the context of current guidelines for addressing these concerns. Methods: An internal survey was developed for the principal investigators of the DISPLACE study to identify developmental-behavioral clinical practices across the 28-site consortium, including methods for identification, referral practices, access to psychologists, and barriers to services. Descriptive data were pulled from the survey to describe practice patterns. Results: Most sites used informal methods to detect developmental-behavioral concerns, though over one third of sites were using a structured protocol. The most common referral indications for further developmental and neuropsychological evaluation were parent, provider, or school concerns or stroke. Evaluations were predominantly completed by pediatric neuropsychologists and pediatric psychologists. Despite most sites reporting access to a psychologist within the SCD clinic, sites also reported long waitlists and difficulty accessing providers for evaluation and treatment services. Insurance difficulties were also a common barrier. A range of additional barriers were reported at the patient, provider, organizational, and policy/socioenvironmental levels. Conclusions: Many sites in the DISPLACE consortium were adhering to existing care guidelines for pediatric SCD; however, there was also wide variation in practices for which guidelines are absent or unclear. Additional work is needed to inform guidelines, to specify the role of psychology within specialty SCD care, and to overcome barriers to care. Implications for Impact Statement Optimal developmental-behavioral care for children with sickle cell disease involves collaboration among specialty care providers and pediatric psychologists; however, these efforts may be hampered by dispersed or unclear guidelines and several barriers to care. Future studies and guidelines have the potential to improve care by providing clear, consistent, and unified care recommendations specific to psychologists and related care professionals who are supporting children with sickle cell disease.
Light-induced tumor theranostics based on chemical-exfoliated borophene
Authors: Xie Zhongjian , Duo Yanhong , Fan Taojian , Zhu Yao , Feng Shuai , Li Chuanbo , Guo Honglian , Ge Yanqi , Ahmed Shakeel , Huang Weichun , Liu Huiling , Qi Ling , Guo Rui , Li Defa , Prasad Paras N. , Zhang Han 
Published: November 2022

AbstractAmong 2D materials (Xenes) which are at the forefront of research activities, borophene, is an exciting new entry due to its uniquely varied optical, electronic, and chemical properties in many polymorphic forms with widely varying band gaps including the lightest 2D metallic phase. In this paper, we used a simple selective chemical etching to prepare borophene with a strong near IR light-induced photothermal effect. The photothermal efficiency is similar to plasmonic Au nanoparticles, with the added benefit of borophene being degradable due to electron deficiency of boron. We introduce this selective chemical etching process to obtain ultrathin and large borophene nanosheets (thickness of ~4 nm and lateral size up to ~600 nm) from the precursor of AlB2. We also report first-time observation of a selective Acid etching behavior showing HCl etching of Al to form a residual B lattice, while HF selectively etches B to yield an Al lattice. We demonstrate that through surface modification with polydopamine (PDA), a biocompatible smart delivery nanoplatform of B@PDA can respond to a tumor environment, exhibiting an enhanced cellular uptake efficiency. We demonstrate that borophene can be more suitable for safe photothermal theranostic of thick tumor using deep penetrating near IR light compared to gold nanoparticles which are not degradable, thus posing long-term toxicity concerns. With about 40 kinds of borides, we hope that our work will open door to more discoveries of this top-down selective etching approach for generating borophene structures with rich unexplored thermal, electronic, and optical properties for many other technological applications.
Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis
Authors: Lin Guowen , Huang Tianrun , Zhang Xiaobo , Wang Gangmin 
Published: November 2022

AbstractThe mutual interplay between epigenetic modifications and metabolic rewiring contributes to malignant features of prostate adenocarcinoma (PRAD). This study aimed to uncover the biological roles of deubiquitylase USP35 in PRAD and find effective epigenetic or metabolic targets. Bioinformatic tools or methods revealed that USP35 is upregulated in PRAD samples and correlates with inferior prognosis. The in vitro and in vivo assays suggested that USP35 could enhance malignant features of PRAD cells. Mechanistically, we found that USP35 could directly deubiquitinate and stabilize BRPF1 proteins. USP35 depends on accumulated BRPF1 proteins to accelerate cell growth, stem-like properties, and migration in vitro and in vivo. Interestingly, high BRPF1 could bind to promoter of SREBP2 and activate the SREBP2 transcriptional capacity. Therefore, USP35/BRPF1 aixs could promote expressions of mevalonate (MVA) metabolism signature in a SREBP2-dependent manner. USP35 depends on BRPF1 to maintain the activity of mevalonate metabolism in PRAD cells. Last of all, we observed that targeting BRPF1 or using MVA inhibitor (atorvastatin) are effective to suppress USP35highPRAD in vivo tumor growth. USP35 is an indicator of MVA metabolic signature in PRAD. Collectively, our study highlighted the USP35/BRPF1/SREBP2 axis in modulating MVA metabolism in PRAD, suggesting the significance of BRPF1 or MVA as the potential therapeutic targets for PRAD treatment.
Vascular endothelium deploys caveolin-1 to regulate oligodendrogenesis after chronic cerebral ischemia in mice
Authors: Zhao Ying , Zhu Wusheng , Wan Ting , Zhang Xiaohao , Li Yunzi , Huang Zhenqian , Xu Pengfei , Huang Kangmo , Ye Ruidong , Xie Yi , Liu Xinfeng 
Published: November 2022

AbstractOligovascular coupling contributes to white matter vascular homeostasis. However, little is known about the effects of oligovascular interaction on oligodendrocyte precursor cell (OPC) changes in chronic cerebral ischemia. Here, using a mouse of bilateral carotid artery stenosis, we show a gradual accumulation of OPCs on vasculature with impaired oligodendrogenesis. Mechanistically, chronic ischemia induces a substantial loss of endothelial caveolin-1 (Cav-1), leading to vascular secretion of heat shock protein 90α (HSP90α). Endothelial-specific over-expression of Cav-1 or genetic knockdown of vascular HSP90α restores normal vascular-OPC interaction, promotes oligodendrogenesis and attenuates ischemic myelin damage. miR-3074(−1)−3p is identified as a direct inducer of Cav-1 reduction in mice and humans. Endothelial uptake of nanoparticle-antagomir improves myelin damage and cognitive deficits dependent on Cav-1. In summary, our findings demonstrate that vascular abnormality may compromise oligodendrogenesis and myelin regeneration through endothelial Cav-1, which may provide an intercellular mechanism in ischemic demyelination.
Biochemical and structural basis for differential inhibitor sensitivity of EGFR with distinct exon 19 mutations
Authors: van Alderwerelt van Rosenburgh Iris K. , Lu David M. , Grant Michael J. , Stayrook Steven E. , Phadke Manali , Walther Zenta , Goldberg Sarah B. , Politi Katerina , Lemmon Mark A. , Ashtekar Kumar D. , Tsutsui Yuko 
Published: November 2022

AbstractTyrosine kinase inhibitors (TKIs) are used to treat non-small cell lung cancers (NSCLC) driven by epidermal growth factor receptor (EGFR) mutations in the tyrosine kinase domain (TKD). TKI responses vary across tumors driven by the heterogeneous group of exon 19 deletions and mutations, but the molecular basis for these differences is not understood. Using purified TKDs, we compared kinetic properties of several exon 19 variants. Although unaltered for the second generation TKI afatinib, sensitivity varied significantly for both the first and third generation TKIs erlotinib and osimertinib. The most sensitive variants showed reduced ATP-binding affinity, whereas those associated with primary resistance retained wild type ATP-binding characteristics (and lowKM, ATP). Through crystallographic and hydrogen-deuterium exchange mass spectrometry (HDX-MS) studies, we identify possible origins for the altered ATP-binding affinity underlying TKI sensitivity and resistance, and propose a basis for classifying uncommon exon 19 variants that may have predictive clinical value.
Faecal microbiome-based machine learning for multi-class disease diagnosis
Authors: Su Qi , Liu Qin , Lau Raphaela Iris , Zhang Jingwan , Xu Zhilu , Yeoh Yun Kit , Leung Thomas W. H. , Tang Whitney , Zhang Lin , Liang Jessie Q. Y. , Yau Yuk Kam , Zheng Jiaying , Liu Chengyu , Zhang Mengjing , Cheung Chun Pan , Ching Jessica Y. L. , Tun Hein M. , Yu Jun , Chan Francis K. L. , Ng Siew C. 
Published: November 2022

AbstractSystemic characterisation of the human faecal microbiome provides the opportunity to develop non-invasive approaches in the diagnosis of a major human disease. However, shared microbial signatures across different diseases make accurate diagnosis challenging in single-disease models. Herein, we present a machine-learning multi-class model using faecal metagenomic dataset of 2,320 individuals with nine well-characterised phenotypes, including colorectal cancer, colorectal adenomas, Crohn’s disease, ulcerative colitis, irritable bowel syndrome, obesity, cardiovascular disease, post-acute COVID-19 syndrome and healthy individuals. Our processed data covers 325 microbial species derived from 14.3 terabytes of sequence. The trained model achieves an area under the receiver operating characteristic curve (AUROC) of 0.90 to 0.99 (Interquartile range, IQR, 0.91–0.94) in predicting different diseases in the independent test set, with a sensitivity of 0.81 to 0.95 (IQR, 0.87–0.93) at a specificity of 0.76 to 0.98 (IQR 0.83–0.95). Metagenomic analysis from public datasets of 1,597 samples across different populations observes comparable predictions with AUROC of 0.69 to 0.91 (IQR 0.79–0.87). Correlation of the top 50 microbial species with disease phenotypes identifies 363 significant associations (FDR < 0.05). This microbiome-based multi-disease model has potential clinical application in disease diagnostics and treatment response monitoring and warrants further exploration.
Transfer learning enhanced water-enabled electricity generation in highly oriented graphene oxide nanochannels
Authors: Yang Ce , Wang Haiyan , Bai Jiaxin , He Tiancheng , Cheng Huhu , Guang Tianlei , Yao Houze , Qu Liangti 
Published: November 2022

AbstractHarvesting energy from spontaneous water flow within artificial nanochannels is a promising route to meet sustainable power requirements of the fast-growing human society. However, large-scale nanochannel integration and the multi-parameter coupling restrictive influence on electric generation are still big challenges for macroscale applications. In this regard, long-range (1 to 20 cm) ordered graphene oxide assembled framework with integrated 2D nanochannels have been fabricated by a rotational freeze-casting method. The structure can promote spontaneous absorption and directional transmission of water inside the channels to generate considerable electric energy. A transfer learning strategy is implemented to address the complicated multi-parameters coupling problem under limited experimental data, which provides highly accurate performance optimization and efficiently guides the design of 2D water flow enabled generators. A generator unit can produce ~2.9 V voltage or ~16.8 μA current in a controllable manner. High electric output of ~12 V or ~83 μA is realized by connecting several devices in series or parallel. Different water enabled electricity generation systems have been developed to directly power commercial electronics like LED arrays and display screens, demonstrating the material’s potential for development of water enabled clean energy.
The 3D structure of lipidic fibrils of α-synuclein
Authors: Frieg Benedikt , Antonschmidt Leif , Dienemann Christian , Geraets James A. , Najbauer Eszter E. , Matthes Dirk , de Groot Bert L. , Andreas Loren B. , Becker Stefan , Griesinger Christian , Schröder Gunnar F. 
Published: November 2022

Abstractα-synuclein misfolding and aggregation into fibrils is a common feature of α-synucleinopathies, such as Parkinson’s disease, in which α-synuclein fibrils are a characteristic hallmark of neuronal inclusions called Lewy bodies. Studies on the composition of Lewy bodies extracted postmortem from brain tissue of Parkinson’s patients revealed that lipids and membranous organelles are also a significant component. Interactions between α-synuclein and lipids have been previously identified as relevant for Parkinson’s disease pathology, however molecular insights into their interactions have remained elusive. Here we present cryo-electron microscopy structures of six α-synuclein fibrils in complex with lipids, revealing specific lipid-fibril interactions. We observe that phospholipids promote an alternative protofilament fold, mediate an unusual arrangement of protofilaments, and fill the central cavities of the fibrils. Together with our previous studies, these structures also indicate a mechanism for fibril-induced lipid extraction, which is likely to be involved in the development of α-synucleinopathies. Specifically, one potential mechanism for the cellular toxicity is the disruption of intracellular vesicles mediated by fibrils and oligomers, and therefore the modulation of these interactions may provide a promising strategy for future therapeutic interventions.
Single cell profiling of primary and paired metastatic lymph node tumors in breast cancer patients
Authors: Liu Tong , Liu Cheng , Yan Meisi , Zhang Lei , Zhang Jing , Xiao Min , Li Zhigao , Wei Xiaofan , Zhang Hongquan 
Published: November 2022

AbstractThe microenvironment of lymph node metastasized tumors (LNMT) determines tumor progression and response to therapy, but a systematic study of LNMT is lacking. Here, we generate single-cell maps of primary tumors (PTs) and paired LNMTs in 8 breast cancer patients. We demonstrate that the activation, cytotoxicity, and proliferation of T cells are suppressed in LNMT compared with PT.CD4+CXCL13+T cells in LNMT are more likely to differentiate into an exhausted state. Interestingly,LAMP3+dendritic cells in LNMT display lower T cell priming and activating ability than in PT. Additionally, we identify a subtype ofPLA2G2A+cancer-associated fibroblasts enriched in HER2+breast cancer patients that promotes immune infiltration. We also show that the antigen-presentation pathway is downregulated in malignant cells of the metastatic lymph node. Altogether, we characterize the microenvironment of LNMT and PT, which may shed light on the individualized therapeutic strategies for breast cancer patients with lymph node metastasis.