bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effects of Teleworking in a Pandemic Context on the Well-Being of People with Disabilities: A Canadian Qualitative Study
Authors: Lecours Alexandra , Gilbert Marie-Hélène , Boucher Normand , Vincent Claude 
Published: July 2022

Abstract
The COVID-19 pandemic has led to numerous changes in work environments. Thousands of workers quickly found themselves having to telework without being prepared, which had consequences on their well-being. Authors proposed telework practices that promote the well-being of workers in a pandemic context, but less attention has been paid to consider the needs of disabled workers. Purpose. This study aimed to explore the effects of telework during the pandemic on the well-being of people with disabilities. Methods. Following an interpretive descriptive research design, interviews were conducted with 16 workers with disabilities (i.e., motor, or sensory). The data were analyzed using a thematic analysis strategy. Results. The results revealed 15 factors that influence the well-being of teleworkers with disabilities. These factors are related to interactions between three spheres of the worker's life: the individual, the organization, and the environment. Ten recommendations are proposed to consider the reality of disabled individuals in the telework practices. Conclusion. Given that telework has expanded since the onset of the COVID-19 pandemic and will likely continue to remain a widespread modality of work delivery, it becomes even more important to expand knowledge about it, to benefit the well-being of disabled teleworkers.
Iron (hydr)oxide formation in Andosols under extreme climate conditions
Authors: Klaes Björn , Thiele-Bruhn Sören , Wörner Gerhard , Höschen Carmen , Mueller Carsten W. , Marx Philipp , Arz Helge Wolfgang , Breuer Sonja , Kilian Rolf 
Published: July 2022

Abstract
Redox-driven biogeochemical cycling of iron plays an integral role in the complex process network of terrestrial ecosystems, such as carbon and phosphorous cycling, the fate of nutrients and greenhouse gas emissions. We investigate Fe-(hydr)oxide (trans)formation pathways from volcanic glass and titanomagnetite phenocrysts in acidic topsoils of South Patagonian Andosols, combining bulk geochemical analyses with micrometer-scale-measurements on individual aggregates. Our data indicate that Fe-(hydr)oxide (trans)formation is closely linked to tephra weathering and organic matter turnover, regulated by frequent hydrological perturbations related to the hyper-humid climate. We document abiotic and microbially-mediated pathways, including Fe-(hydr)oxide nucleation after glass dissolution and complexation with organic ligands, maghemitization and dissolution-(re)crystallization processes from metastable precursors. Ultimately, hematite represents the sole thermodynamically stable Fe-(hydr)oxide formed under these conditions. The insights into iron cycling in pristine Andosols have implications for a better understanding of the colloidal delivery of bio-available iron and carbon sequestration in the unique Patagonian fjord ecosystem.
Genetically modified MSCs into neural and endothelial cell-like cells induced by a branched peptide amphiphile hydrogel
Authors: Li Yixiu , Yin Peiyi , Yu Wang , Zhou Tiantian , Jiang Xinghai , Wu Kai , song yulin 
Published: July 2022

Abstract
Background： Spinal cord injury (SCI) remain one of the great clinical challenges because of their considerable long-term disability potential. In this study, A branched amphiphilic peptide (B-PA) segments were constructed and self-assembled into hydrogels to support bone marrow derived mesenchymal stromal cells (BMSCs) encoding Recombinant Rat VEGF165（VEGF165）, neurotrophins-3(NT-3)and angiopoietin-1(ANG-1) genes. In addition, B-PAS promoted the transdifferentiation of BMSCs into nerve and vascular endothelial cells. Methods：Self-assembly of b-PA into a nanofiber hydrogel was triggered by the culture medium and analysed by transmission electron microscopy (TEM). Rat BMSCs were cultivated using the differential adhesion method and assessed by flow cytometry. Cells were inoculated into the hydrogel and investigated using calcein-AM/PI, CCK-8, and cell adhesion assays. Cells transfected with adenoviral vectors of biocarrier (Adv-Bic) and adenoviral vectors of NT-3 gene (AdvNT-3) were cultured for 7 days and evaluated by immunofluorescence and real time quantitative reverse transcription-PCR. Results：The molecular weight and purity of b-PA were 2191.72 and >95%, respectively. Self-assembled nanofibers had a diameter of 5-8 nm and a length ranging from hundreds of nanometres to micrometres. The resulting CD29+/44+ cells could differentiate into osteoblasts and fat cells, and the hydrogel supported cell growth, proliferation and adhesion. Cells in the hydrogel overexpressed VEGF165, NT-3 and Ang-1, and NSE and Nestin markers of CD31+/34+ cells were also abundant. Conclusions： The hydrogel has potential as a tissue engineering scaffold with good cytocompatibility, and can induce genetically modified BMSCs to transdifferentiate into neural and endothelial cell (EC)-like cells.
Intraspecific Variations in Leaf Functional Traits of Cunninghamia lanceolata provenances
Authors: Xu Rui , Cheng Shundan , Zhou Jing , Tigabu Mulualem , Ma Xiangqing , LI MING 
Published: July 2022

Abstract
Background
Studies on intra-specific variability in leaf functional traits is important to evaluate adaptation of the species to predicted climate change, and to develop long-term conservation strategy. The main objectives were to investigate the relationship between the functional traits leaves and C, N, P stoichiometry of Chinese fir from different geographical provenances and their relationship with the main environmental factors of provenance.
Results
In this study, we measured 12 leaf functional traits on 36-year-old Cunninghamia lanceolata trees from 13 provenances. Analysis of variance (ANOVA) was performed to examine the variability. Redundancy analysis (RA) was computed to examine the relationship between geo-climatic factors of provenance origin and leaf functional traits while Pearson’s correlation coefficient was computed to assess inter-trait correlations. The results showed statistically significant differences (P < 0.01) in intraspecific leaf traits among provenances, except leaf P content. The relationships among leaf traits are consistent with the general trend observed in the leaf economic spectrum. Mean annual temperature appeared to be a key factor that influences intraspecific leaf traits variability compared to mean annual precipitation.
Conclusion
These results provide useful insights about adaptation of leaf trait of Chinese fir in a changing climatic condition. Thus, our findings shed light on the importance of interspecific trait variability in Chinese fir and the potential effect of climate change.
Ellagic Acid Alleviates Mice intestinal ischemia-reperfusion injury: A Study Based on Transcriptomics Combined with Functional Experiments
Authors: Fan Shuyuan , Xu yue , Li kun , Li bin , Diao yunpeng 
Published: July 2022

Abstract
Background：It has been reported that intestinal ischemia-reperfusion injury (IIRI) is closely related to inflammatory response, apoptosis and oxidative stress. Ellagic acid (EA) has been proved to have antioxidant and anti-inflammatory effects, and can inhibit tumor angiogenesis. The purpose of this study was to investigate the protective effects of EA on IIRI in mice.Methods：A mouse model of IIRI was established by clamping the mesenteric artery. Effects and mechanisms of EA on IIRI were investigate by transcriptomics combined with functional experiments. Results: The symptoms of IIRI were reflected in significant increases in inflammatory factors such as TNF-α and IL-1β; significant increases in oxidative stress indicators such as MDA and GSH and decreases in SOD and promotion of the apoptotic protein Bax/Bcl-2. These indicators were significantly alleviated by EA. And after EA treatment, transcriptomics results identified AKT1 differentially expressed mRNAs mainly enriched in PI3K/AKT signalling pathway. Conclusion: This study illustrates the protective effects against IIRI, the possible mechanisms were also studied. This study provides new scientific information for the application of EA in IIRI therapy.
Sustainable total factor productivity of transport: considering safety issues and environmental impacts
Authors: Lu Mingxuan , Chen Peirong 
Published: July 2022

Abstract
Economy, environment and safety are three important components of sustainable transport. This paper proposes a productivity measurement standard that comprehensively considers economic growth, environmental impact and safety issues, namely sustainable total factor productivity (STFP). We measure the growth rate of STFP in transport sector of OECD countries in terms of Malmquist-Luenberger productivity index by applying data envelopment analysis (DEA). It is found that the growth rate of total factor productivity in transport sector can be overestimated if safety is ignored. In addition, we discuss the influence of socio-economic factors on the measurement results, finding that there exists a threshold on the impact of environmental regulation intensity on the growth of STFP in transport. That is, STFP increases with environmental regulation intensity if it is smaller than 0.247, while STFP decreases if it is larger than 0.247.
A kinesin mediates VEGFR2 recycling and regulates VE-cadherin phosphorylation, essential for vascular permeability
Authors: Cho Hyun-Dong , Zhou Christopher , Tu Kayeman , Nguyen Tara , Sarich Nicolene A. , Yamada Kaori 
Published: July 2022

Abstract
Excessive vascular endothelial growth factor-A (VEGF-A) signaling induces vascular leakage and angiogenesis in diseases. VEGFR2 trafficking to the cell surface, mediated by kinesin-3 family protein KIF13B, is essential to respond to VEGF-A in inducing angiogenesis. However, the precise mechanism of how KIF13B regulates VEGF-induced signaling and endothelial permeability is unknown. Here we show that KIF13B-mediated recycling of internalized VEGFR2 through Rab11-positive recycling vesicle regulates VE-cadherin phosphorylation and endothelial permeability. Phosphorylated VEGFR2 at the cell-cell junction was internalized and associated with KIF13B in Rab5-positive early endosomes. KIF13B mediated VEGFR2 recycling through Rab11-positive recycling vesicle, and inhibition of this recycling attenuated phosphorylation of VEGFR2 at Y951, Src, and VE-cadherin at Y685, which are necessary for endothelial permeability. Failure of VEGFR2 trafficking to the cell surface induced accumulation and degradation of VEGFR2 in lysosomes. Furthermore, in the animal model of wet age-related macular degeneration (AMD), inhibition of KIF13B-mediated VEGFR2 trafficking also mitigated vascular leakage. Thus, the present results identify the fundamental role of VEGFR2 recycling to the cell surface in mediating vascular permeability, suggesting a promising strategy for mitigating vascular leakage associated with inflammatory diseases.
Depression, Anxiety, &amp; Loneliness Among Cancer Survivors During the COVID-19 Pandemic
Authors: White Larissa Lee , Goldberg-Scott Shauna , Feigelson Heather Spencer , Burnett-Hartman Andrea N. 
Published: July 2022

Abstract
Purpose: Although studies have evaluated the mental health impacts in those recently diagnosed with cancer during the pandemic, few studies focused on cancer survivors who are past their primary cancer treatment. The objective of this study was to assess the impact of the COVID-19 pandemic on depression, anxiety, and loneliness between those with and without a history of cancer.Methods: Kaiser Permanente Research Bank participants were invited to complete a series of surveys from May to December 2020. The difference in score of depression, anxiety, and loneliness were estimated using linear mixed regression. All models adjusted for age group, sex, race/ethnicity, Kaiser Permanente region, smoking status, and Charlson comorbidity score.Results: Among cancer survivors, 21% and 19% met the thresholds for increased risk of depression and anxiety. Among those without a history of cancer, 24% met the thresholds for increased risk of depression and anxiety. Both groups reported decreases in mean depression, anxiety, and loneliness scores between May and July 2020, however, scores increased from August to December 2020. Cancer survivors had decreased depression, anxiety, and loneliness scores compared to those without a history of cancer. Among cancer survivors, younger age groups and females reported increased depression, anxiety, and loneliness scores, while non-Hispanic Black and Asian participants reported decreased depression, anxiety, and loneliness scores.Conclusion: The results of this study highlight the continued necessity of addressing mental health needs and social support in cancer survivors, especially those who are younger and female, during and after a public health emergency.
Using Hand Painting in the Fabrication of a Negative Refractive Index Metamaterial Based on Circular Shape with Paper as the Dielectric
Authors: Hamonangan Felix R.M. , Yudistira Hadi Teguh , Qalbina Fitrah , Saputro Adhitya Gandaryus , Faisal Amir 
Published: July 2022

Abstract
This study aims to use the hand-painting method in designing and fabricating a metamaterial based on a circular shape that exhibits a negative refractive index over the microwave spectrum. Hand painting is a simple and inexpensive fabrication method. This study applies silver ink as a periodic conductor to glossy paper as the dielectric substrate. The spectrum of investigation in this study is 3–6 GHz, and the experimental results obtained are compared with the simulation results. The slight error obtained between transmission results from the simulation and the experiments reflects limitations in the fabrication methods used. Overall, the experimental results present good agreement with the simulation results. The electrical properties of the metamaterial are retrieved from the transmission and reflection simulation results. The simulation results are employed instead of the experimental results because of the fluctuating data provided by the latter. The refractive index presents a negative value at 3–3.6 GHz.
Effect of the Aramid Pulp on the Physicochemical, Viscoelastic Properties and Rheokinetics of Polyurethanes
Authors: Cruz Joziel Aparecido , Amico Sandro C. , Bianchi Otávio 
Published: July 2022

Abstract
This publication highlights the effect of aramid pulp (1 wt.%) on equilibrium swelling, enthalpy reaction, solid viscoelastic properties, and rheokinetics of castor oil (CO) and polyether (PE) polyol blend (50/50 CO/PE) with polymeric isocyanate (pMDI). The CO-pMDI system showed a lower solubility parameter difference (Δδi) than the PE-pMDI and CO/PE-pMDI. Crosslink density values νe are higher for CO-pMDI, and when added to aramid pulp, there is a slight increase due to the possible restriction of swollen volume. Regarding the pseudo-solid behavior, AP restricts the macromolecular movement and confers reinforcing characteristics to PU systems. The Prout–Tompkins autocatalytic model satisfactorily described the reactions, resulting in activation energy values Ea within 45−54 kJ/mol. However, for the CO/PE blend, the difference between the reactivity of the polyols and the solubility parameter with pMDI results in a two-step reaction kinetics. Aramid pulp practically does not change the reaction mechanism, only the viscosity of the medium. Furthermore, the thermodynamic parameters (ΔS* and ΔH*) indicated that the PU with AP resulted in more ordered complexes. The adequate description of the polymerization reaction and the effect of the aramid pulp allowed obtaining PU formulations with tunable characteristics for strict composite processing composites.
High-performance glass filters for capturing and culturing circulating tumor cells and cancer-associated fibroblasts
Authors: Tanaka Hiromasa , Iwata Daijiro , Shibata Yuki , Hase Tetsunari , Onoshima Daisuke , Yogo Naoyuki , Shibata Hirofumi , Sato Mitsuo , Ishikawa Kenji , Nagasawa Ikuo , Hasegawa Yoshinori , Ishii Makoto , Baba Yoshinobu , Hori Masaru 
Published: July 2022

Abstract
Various liquid biopsy methods have been developed for the non-invasive and early detection of diseases. In particular, the detection of circulating tumor cells (CTCs) and cancer-associated fibroblasts (CAFs) in blood has been receiving a great deal of attention. We have been developing systems and materials to facilitate such liquid biopsies. In this study, we further developed glass filters (with various patterns of holes, pitches, and non-adhesive coating) that can capture CTCs, but not white blood cells. We optimized the glass filters to capture CTCs, and demonstrated that they could be used to detect CTCs from lung cancer patients. We also used the optimized glass filters for detecting CAFs. Additionally, we further developed a system for visualizing the captured cells on the glass filters. Finally, we demonstrated that we could directly culture the captured cells on the glass filters. Based on these results, our high-performance glass filters appear to be useful for capturing and culturing CTCs and CAFs for further examinations.
Metagenome profile reveals changes in hydrocarbonoclastic bacterial community composition enriched at different temperatures
Authors: Singha Ningombam A. , Neihsial Roselin , Kipgen Lhinglamkim , Lyngdoh Waniabha J. , Nongdhar Jopthiaw , Singh Prabhakar , Chettri Bobby , Singh Arvind K. 
Published: July 2022

Abstract
The variation in temperature in an ecosystem significantly impact the bacterial community composition and their functional genes. Such changes in the microbiota are well investigated using 16S rDNA metagenomic approach. We report crude oil (CO) degradation activity and metagenome profile of four hydrocarbonoclastic bacterial consortia enriched from an oily sludge at 4±2 ˚C (3% w/v CO), 25±2 ˚C (1% and 3% w/v CO), and 45±2 ˚C (3% w/v CO) in Bushnell-Haas medium. The total petroleum hydrocarbon (TPH) removal and hydrocarbon degradation activity was found maximum for consortia raised at 25±2 ˚C and minimum for consortium enriched at 4±2 ˚C. The consortium developed at 25±2 ˚C showed alkane hydroxylase and catechol 2, 3-dioxygenase activities also. The 16S metagenomic approach revealed predominance of Proteobacteria (62.12%-91.25%) at all temperatures followed by Bacteroidetes (18.94%-37.77%) at 4±2 ˚C and 25±2 ˚C. The consortium at 45±2 ˚C showed a diverse community structure comprising 7.38% Actinobacteria and 0.41% Bacteroidetes. Further significant variation in overall community composition and relative abundance was observed between consortia at genus level. The functional analysis predicted 247 KOs mapped in xenobiotic biodegradation and metabolism pathways involved in hydrocarbon degradation. This study revealed changes in bacterial community composition and metabolic construction in response to variation in temperature and hydrocarbon concentration.
Bioenergetic control of soil carbon dynamics across depth
Authors: Henneron Ludovic , Balesdent Jerôme , Alvarez Gaël , Barré Pierre , Baudin François , Basile-Doelsch Isabelle , Cécillon Lauric , Fernandez-Martinez Alejandro , Hatté Christine , Fontaine Sébastien 
Published: July 2022

Abstract
Soil carbon dynamics is strongly controlled by depth globally, with increasingly slow dynamics found at depth. The mechanistic basis remains however controversial, limiting our ability to predict carbon cycle-climate feedbacks. Combining radiocarbon and thermal analyses of soil carbon with long-term incubations in absence/presence of continuously 13C/14C-labelled plants, we show here that bioenergetic constraints of decomposers consistently drive the depth-dependency of soil carbon dynamics over a range of mineral reactivity context. The slow dynamics of subsoil carbon was tightly related to both its low energy density and high activation energy of decomposition, leading to an unfavorable ‘return-on-energy-investment’ for decomposers. We also observed strong acceleration of millennia-old subsoil carbon decomposition induced by roots (‘rhizosphere priming’), showing that sufficient supply of energy by roots is able to alleviate the strong energy limitation of decomposition. These findings demonstrate that the slow dynamics of subsoil carbon results from its poor energy quality together with the lack of energy supply by roots at depth.
A novel mutation, c.1342T&gt;C (p.Cys448Arg) identified from an infant with fever-triggered recurrent acute liver failure, affects expressions of NBAS mRNA and protein and induces cellular stress in vitro
Authors: Ji Juhua , Yang Mingming , JunJun Jia , Wu Qi , Cui Hengxiang , Zhu Baofeng , Chu Xin 
Published: July 2022

Abstract
The mutations in neuroblastoma amplified sequence (NBAS) gene correlate with two clinical spectra: short stature with optic nerve atrophy and Pelger-Huët anomaly (SOPH) syndrome and infantile liver failure syndrome 2 (ILFS2). Here, we describe a study of a 26-month-old Chinese girl who was diagnosed with fever-triggered recurrent acute liver failure (ALF). The clinical characteristics were great elevation of liver enzymes, severe coagulopathy, and acute renal failure. Whole-exome and Sanger sequencing of the patient and his parents revealed that she carried novel compound heterozygous missense mutations in NBAS c.938_939delGC and c.1342T > C (p.Cys448Arg). The frameshift mutation c.[938_939delGC] should cause truncated NBAS protein without normal function and c.1342T > C (p.Cys448Arg) mutation affects evolutionarily conserved amino acid residues. And we found the ratio of peripheral CD3+CD4+CD45+ to CD3+CD8+CD45+ was lower in patient than that in normal children, and c.1342T > C mutation reduced expression of NBAS mRNA and protein, enriched intracellular reactive oxygen species, and induced cell apoptosis and endoplasmic reticulum stress in vitro cell models. Our study would enrich our understanding of the mechanism of NBAS mutation in regulating the ALF progress. We recommend NBAS gene analysis in children with unexplained fever-triggered recurrent ALF or liver dysfunction.
Comparison of 30-day planned and unplanned readmissions to huge hospital in China
Authors: Zhang Mengjiao , Liu Siru , Bi Yongdong , Liu Jialin 
Published: July 2022

Abstract
Purpose: To compare and analyze the clinical characteristics and influencing factors of planned and unplanned readmissions within 30 days to provide a basis for the quality of care management.Methods: We searched for inpatients at West China Hospital from January 1, 2015, to December 31, 2020. Patients were divided into unplanned readmission and planned readmission groups according to 30-day readmission status. Patient demographics and related information were collected for each patient.Results: We identified 1,242,496 hospitalized patients, including 74,494 (6.0%) 30-day planned readmissions and 9,895 (0.8%) unplanned readmissions. There were statistically significant differences between planned and unplanned readmissions in terms of patient gender, marital status, age, length of initial stay, the time between discharge, stay in ICU, surgery, and health insurance.Conclusion: Accurate information on 30-day planned and unplanned readmissions facilitates effective planning of healthcare resource allocation. Identify risk factors for 30-day unplanned readmission and develop interventions to reduce readmission rates.