bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adaptation and implementation outcomes of a parenting program for low-income, ethnically diverse families delivered virtually versus in-person
Authors: Grafft Natalie , Aftosmes-Tobio Alyssa , Gago Cristina , Lansburg Kindra , Beckerman-Hsu Jacob , Trefry Brooke , Kumanyika Shiriki , Davison Kirsten 
Published: November 2022

Abstract
Driven by the COVID-19 pandemic, many in-person health behavior interventions were compelled to quickly pivot to a virtual format with little time or capacity to reflect on or examine possible equity-related implications of a format that required digital access and remote learning skills. Using a parenting program for low-income families as a case study, this paper (a) outlines the process of adapting the program from an in-person to a virtual format and (b) examines the equity-related implications of this adaptation. Parents Connect for Healthy Living (PConnect) is a 10-session empowerment-focused parenting intervention designed to promote family health for Head Start families. In 2020, PConnect was adapted over a 6-month period from an in-person to a virtual format due to the advent of the COVID-19 pandemic. Three core elements were retained in the adaptation; session content, provision of coaching support for facilitators, and the co-facilitation model. Key modifications include session length, group composition, and language of program delivery. Head Start and PConnect records provided data to compare reach, acceptability, and appropriateness of virtual and in-person PConnect. Seventy-eight parents enrolled in the in-person program and 58 in the virtual program. Participant demographics and satisfaction were similar across formats, and demographics similar to the general Head Start population. Participation was higher in the virtual format. Parents participated in the virtual program primarily via smart phones (68%). This case study supports the acceptability and appropriateness of virtual parenting programs in ethnically diverse, low-resource settings.
Characteristics and Limitations of Video-capillaroscopy in Reconstructive Microsurgery for Different Histologic Components of Flaps
Authors: Escandón Joseph M. , Mohammad Arbab , Matsui Chihiro , Tanaka Takakuni , Wei-Kai Lao William , Yoshitsugu Hattori , Matsui Yuki , Mizuno Hiroshi 
Published: November 2022

Summary:
Indocyanine green, ultrasonography, and handheld Doppler can be used to evaluate blood flow at the donor and recipient site during microvascular reconstruction. However, these methods do not provide direct visualization and assessment of real-time blood flow. Video-capillaroscopy has been shown to be useful in clinical practice to assess microcirculation in rheumatologic disorders. In this report we used video-capillaroscopy to assess different tissue components involved in microvascular surgery. Seven patients who underwent head and neck oncologic microvascular reconstruction between November 2021 and February 2022 were included in this study. Video-capillaroscopy (GOKO-BscanZD, GOKO Imaging Devices Co., Ltd., Japan) was used to evaluate the donor-site and recipient-site tissue components. Optimal red blood cell movement was graded with a score of four, while no flow was graded with a score of 0. Seven myocutaneous flaps and seven recipient sites were evaluated. For the donor-site, our analysis demonstrated a significantly higher video-capillaroscopy quality for skin (3.43), adipose tissue (3.7) and perforators (3.7) when compared with muscle (0.429), muscle fascia (0.857), and de-epithelialized skin (1) (P < 0.001). For the recipient-site, a significantly higher video-capillaroscopy quality for skin (2.7), adipose tissue (3.5), and the periosteum (2.1) was noted when compared with muscle (0) (P < 0.001). Video-capillaroscopy efficiency is limited in the muscular component and injured (de-epithelialized) skin surface areas of flaps. Herein, we provide evidence that assessment of flap perfusion with video-capillaroscopy can be reliably achieved in the skin, periosteum, perforators, and adipose tissue. Video-capillaroscopy is expected to be applied for intraoperative real-time blood flow evaluation.
A Systematic Review on Antituberculosis Drug Discovery and Antimycobacterial Potential of Biologically Synthesized Silver Nanoparticles: Overview and Future Perspectives
Authors: Ezeh Christian K. , Eze Chibuzor N. , Dibua Uju M. E. , Emencheta Stephen C. 
Published: November 2022

Abstract
Rapid emergence and quick evolution of drug-resistant and aggressive mycobacterial strains have resulted in the present antimycobacterial drug crisis and the persistence of tuberculosis as a major public health problem. Green/biological nanotechnologies constitute an interesting area of research for discovering antimycobacterial agents. This review focused on the biological (green) synthesis of silver nanoparticles (AgNPs) as an alternative source of antimycobacterial agents. Data for this study were searched and screened from three electronic databases (Google Scholar, PubMed and ScienceDirect) following the Preferred Reporting Items for Systematic Reviews and Meta-analyses flowchart. Data from in total 17 eligible studies were reported in this systematic review. Twelve of the 17 studies used plants to fabricate AgNPs, whereas the remaining five studies used microorganisms (bacteria and/or fungi). Silver as part of silver nitrate (AgNO3) was the metal precursor reported for the synthesis of AgNPs in these studies. Silver nanoparticles were mostly spherical, with sizes ranging from 12 to 140 nm. Results based on minimum inhibitory concentrations varied between studies and were divided into three groups: (i) those more effective than the antibiotic (controls), (ii) those more effective than plant extracts, and (iii) those less effective than the antibiotic controls. In addition, little or no cytotoxicity effects were reported. Silver nanoparticles were also shown to be highly specific or selective toward mycobacterial strains. This systematic review highlights the antimycobacterial potential of biologically synthesized AgNPs, underscoring the possibility of discovering/developing new antimycobacterial agents using biological synthesis approaches with less toxicity and high selectivity.
Transformative Learning Emerging From Challenges First-Year Students Experienced
Authors: Plack Margaret M. , Hilliard Marjorie Johnson , Costello Ellen , Huhn Karen , Maring Joyce , Healey William E. 
Published: November 2022

Introduction.
Doctor of physical therapy (PT) (DPT) programs are rigorous, and students report facing overwhelming challenges. Faculty may not be cognizant of the extent of these challenges and miss opportunities to support student learning. The purpose of this article is to describe factors affecting student coping abilities and the lessons they learned from managing their self-identified challenges.

Review of Literature.
Given the growing body of evidence surrounding mental health issues in DPT students, educators are exploring ways to support student well-being and promote their professional development.

Subjects.
This study is a component of a larger multisite study of first-year DPT students from 3 private universities.

Methods.
Participants submitted written narratives in response to a critical incident questionnaire designed to better understand first-year challenges. Responses were deidentified, researchers were blinded to participation, and confidentiality was maintained throughout. A consensus-driven interpretivist approach to qualitative data analysis was used. Strategies to ensure trustworthiness included triangulation of researchers, peer review, prolonged engagement, and use of thick rich descriptions.

Results.
Seventy responses were analyzed. Two major themes are presented: (1) students described factors internal and external to the learning environment that inhibited and facilitated their ability to cope with challenges and (2) students shared academic successes and lessons learned from overcoming challenges, including the development of new behaviors, enhanced self-awareness, and personal and professional growth.

Discussion and Conclusion.
Building on previous work, analysis of the lived experiences of first-year DPT students revealed a process of transformational learning through challenge. This process highlights the importance of recognizing and supporting the significant incidental learning that occurs in our students during their journey through PT school. Faculty focusing solely on content knowledge, skills, and even critical thinking may not recognize and support the incidental learning occurring and may be missing significant transformational learning opportunities.
Influence of Respiration on Collateral Flow in the Fontan Population Using Real-time Phase-contrast Cardiovascular Magnetic Resonance
Authors: Blessing Maja , Körperich Hermann , Barth Peter , Michel Miriam , Dubowy Otto , Forreiter Sophia , Laser Kai Thorsten 
Published: November 2022

Purpose:
The clinical significance of collateral flow for the ventricular function of patients with univentricular hearts is often debated. This study evaluates the impact of collateral flow on respiration-dependent preload modification and diastolic function in Fontan patients assessed by systemic and pulmonary vein (PV) flow patterns.

Materials and Methods:
Real-time phase-contrast cardiovascular magnetic resonance was performed in the right upper PV, ascending aorta, superior, and inferior vena cava (IVC) in 21 Fontan patients and 11 healthy individuals. The patients’ respiratory cycle was divided into 4 periods to generate respiratory-dependent stroke volumes (SVi). Conventional quantitative blood flow measurements were used to quantify and differentiate between low (group A) and high (group B) collateral flow.

Results:
Group B showed significantly lower SVi IVC in inspiration, end-inspiration, expiration, and SVi ΔIVC compared with group A (23.6±4.8 mL/m2 to 33.4±8.0; P=0.005). PV flow resulted in a lower mean SVi PV (11.6±7.6 mL/m2, vs. 14.0±11.4 mL/m2) as well as a significantly lower peak systolic S-wave velocity (Smax) (P=0.005), S/D-ratio (Smax/peak diastolic wave velocity) (P=0.015), and shorter diastolic deceleration time (DTD; P=0.030; median DTD=134 ms) compared with group A (DTD=202 ms).

Conclusions:
This study demonstrates the incapability of Fontan patients to properly increase preload by inspiration in the presence of significant collateral flow. The results further show that collateral flow is associated with a volume-deprived ventricle and impaired diastolic function.
Violation of the constant genetic effect assumption can result in biased estimates for non-linear Mendelian randomization
Authors: Burgess Stephen 
Published: November 2022

AbstractNon-linear Mendelian randomization is an extension of conventional Mendelian randomization that performs separate instrumental variable analyses in strata of the study population with different average levels of the exposure. The approach estimates a localized average causal effect function, representing the average causal effect of the exposure on the outcome at different levels of the exposure. The commonly-used residual method for dividing the population into strata works under the assumption that the effect of the genetic instrument on the exposure is linear and constant in the study population. However, this assumption may not hold in practice. We use the recently developed doubly-ranked method to re-analyse various datasets previously analysed using the residual method. In particular, we consider a genetic score for 25-hydroxyvitamin D [25(OH)D] used in a recent non-linear Mendelian randomization analysis to assess the potential effect of vitamin D supplementation on all-cause mortality. We show that the effect of the genetic score on 25(OH)D concentrations varies strongly, with a five-fold difference in the estimated genetic association with the exposure in the lowest and highest decile groups. Evidence for a protective causal effect of vitamin D supplementation on all-cause mortality in low vitamin D individuals is evident for the residual method, but not for the doubly-ranked method. We show that the constant genetic effect assumption is more reasonable for some exposures, and less reasonable for others. If the doubly-ranked method indicates that this assumption is violated, then estimates from both the residual and doubly-ranked methods can be biased, although bias was smaller on average in the doubly-ranked method. Analysts should compare results from both methods, as well as considering transforming the exposure to reduce heterogeneity in the genetic effect on the exposure.
AnRt- based model for predicting multiple epidemic waves in a heterogeneous population
Authors: Romanescu Razvan , Hu Songdi , Nanton Douglas , Torabi Mahmoud , Tremblay-Savard Olivier , Haque Md Ashiqul 
Published: November 2022

AbstractRelaxing the homogeneous mixing assumption in a population is often necessary to improve fits of epidemic models to observed infection counts. Establishing a link between observed infections and the underlying network of contacts is paramount to understanding how the network structure affects the speed of spread of a pathogen. In this paper we argue that introducing a flexible structure for the effective reproductive number (Rt) over the course of an epidemic allows for a more realistic description of the network of social contacts. This, in turn, produces better retrospective fits, as well as more accurate prospective predictions of observed epidemic curves. We extend this framework to fit multi-wave epidemics, and to accommodate public health restrictions on mobility. We demonstrate the performance of this model by doing a prediction study over two years of the SARS-CoV2 pandemic.
Augmenting TCR signal strength and ICOS costimulation results in metabolically fit and therapeutically potent human CAR Th17 cell therapy
Authors: Wyatt Megan M. , Huff Logan W. , Nelson Michelle H. , Neal Lillian R. , Medvec Andrew R. , Rangel Rivera Guillermo O. , Smith Aubrey S. , Reyes Amalia M. Rivera , Knochelmann Hannah M. , Riley James L. , Lesinski Gregory B. , Paulos Chrystal M. 
Published: November 2022

ABSTRACTAdoptive cell transfer (ACT) therapy with IL-17 producing human T cells elicits potent antitumor activity in preclinical models. However, further refinement of this novel approach is needed to position it for clinical application. While activation signal strength differentially regulates IL-17 production by human CD4+T cells, the degree to which TCR and co-stimulation signal strength impacts antitumor Th17 cell immunity remains poorly understood. We discovered that decreasing TCR/co-stimulation signal strength by incremental reduction of αCD3/co-stimulation beads in a Th17 culture progressively diminished their effector memory phenotype but enhanced their polyfunctionality. Additional investigation revealed that Th17 cells stimulated with αCD3/ICOS beads produced more IL-17A, IFNγ, IL-2 and IL-22 than those stimulated with αCD3/CD28 beads, regardless of signal strength. Th17 cells propagated with 30-fold fewer αCD3/ICOS beads (weak signal strength, 1 bead per 10 T cells) were less reliant on glucose for growth compared to those stimulated with the standard, strong signal strength (3 beads per T cell). Further metabolomic analysis revealed Th17 cells weakly simulated with αCD3/ICOS beads favored the central carbon pathway through increased gluconeogenesis for bioenergetics, marked by abundant intracellular phosphoenoylpyruvate (PEP). Importantly, Th17 cells weakly stimulated with αCD3/ICOS beads and redirected with a chimeric antigen receptor (CAR) that recognizes mesothelin were more effective at clearing large human mesothelioma tumors when infused into mice than those manufactured using the standard FDA-approved protocols. Taken together, these data indicate Th17 ACT therapy can be improved by using fewer activation beads during T cell manufacturing, a finding that is both cost effective and directly translatable to patients.
The mesodermal source of fibronectin is required for heart morphogenesis and cardiac outflow tract elongation by regulating cell shape, polarity, and mechanotransduction in the second heart field
Authors: Arriagada Cecilia , Lin Evan , Schonning Michael , Astrof Sophie 
Published: November 2022

AbstractFailure in the elongation of the cardiac outflow tract results in congenital heart disease due to ventricular septum defects and misalignment of the great vessels. The cardiac outflow tract lengthens via accretion of progenitors derived from the second heart field (SHF). SHF cells in the splanchnic mesoderm are exquisitely regionalized and organized into an epithelial-like layer forming the dorsal pericardial wall (DPW). Tissue tension, cell polarity, and proliferation within the DPW are important for the addition of SHF-derived cells to the heart and elongation of the cardiac outflow tract. However, the genes regulating these processes are not completely characterized. Using conditional mutagenesis in the mouse, we show that fibronectin (Fn1)synthesized by the SHF is a central regulator of epithelial architecture in the DPW.Fn1is enriched in the anterior DPW and mediates outflow tract elongation by balancing pro- and anti-adhesive cell-ECM interactions and regulating DPW cell shape, polarity, cohesion, proliferation, and mechanoresponsiveness. Our studies establish that Fn1 synthesized specifically by the mesoderm coordinates multiple cellular behaviors in the anterior DPW necessary for elongation of the cardiac outflow tract.
TRPV1-mediated sonogenetic neuromodulation of motor cortex in freely moving mice
Authors: Xu Kevin , Yang Yaoheng , Hu Zhongtao , Yue Yimei , Cui Jianmin , Culver Joseph P. , Bruchas Michael R. , Chen Hong 
Published: November 2022

AbstractBackgroundNoninvasive and cell-type-specific neuromodulation tools are critically needed for probing intact brain function. Sonogenetics for noninvasive activation of neurons engineered to express thermosensitive transient receptor potential vanilloid 1 (TRPV1) by transcranial focused ultrasound (FUS) was recently developed to address this need. However, using TRPV1-mediated sonogenetics to evoke behavior by targeting the cortex is challenged by its proximity to the skull due to high skull absorption of ultrasound and increased risks of thermal-induced tissue damage.ObjectiveThis study evaluated the feasibility and safety of TRPV1-mediated sonogenetics in targeting the motor cortex to modulate the locomotor behavior of freely moving mice.MethodsAdeno-associated virus was delivered to the mouse motor cortex via intracranial injection to express TRPV1 in excitatory neurons. A wearable FUS device was installed on the mouse head after a month to control neuronal activity by activating virally expressed TRPV1 through FUS sonication at different acoustic pressures. Immunohistochemistry staining ofex vivobrain slices was performed to verify neuron activation and evaluate safety.ResultsTRPV1-mediated sonogenetic stimulation at 0.7 MPa successfully evoked rotational behavior in the direction contralateral to the stimulation site, activated cortical neurons as indicated by the upregulation of c-Fos, and did not induce significant changes in inflammatory or apoptotic markers (GFAP, lba1, and Caspase-3). Sonogenetic stimulation of TRPV1 mice at a higher acoustic pressure, 1.1 MPa, induced significant changes in motor behavior and upregulation of c-Fos compared with FUS sonication of naïve mice at 1.1 MPa. However, signs of damage at the meninges were observed at 1.1 MPa.ConclusionsTRPV1-mediated sonogenetics can achieve effective and safe neuromodulation at the cortex with carefully selected FUS parameters. These findings expand the application of this technique to include superficial brain targets.
Pan-cancer Analysis Predicts Kindlin-associated Global Mechanochemical Perturbation
Authors: Chowdhury Debojyoti , Mistry Ayush , Bhatia Riti , Wadan Simran , Chakraborty Soham , Haldar Shubhasis 
Published: November 2022

AbstractKindlins are mechanosensitive adapter proteins that connect extracellular mechanical cues to intracellular chemical events. Any alterations in these proteins thus alter cellular signaling, which could result in cancer progression. However, their involvement in global mechanochemical signals remains elusive in cancers. Here we analyze pan-cancer samples to decipher how kindlin alterations aid cancer progression. We show that kindlin alterations, at both the genetic and mRNA level, dysregulates cellular behavior which significantly correlate with poor survival. We find that while these alterations are cancer-specific, they are prevalent in advanced tumor stages and metastatic onset. We observe that kindlins co-alter with a substantial fraction of human mechanochemical proteome in various tumors. Our analysis suggests how kindlin alterations aid tumor-promoting signals with a synergistic effect from alterations of cancer-hallmark genes. Notably, we demonstrate a consistent alteration of epithelial-mesenchymal-transition markers with kindlin activity. Overall, our study highlights how kindlin alterations could affect metabolism, genomic instability, and signal disruption via their interactome network, causing cancer and suggests targeting them as a therapeutic strategy.
USP10 strikes down β-catenin by dual-wielding deubiquitinase activity and phase separation potential
Authors: Wang Yinuo , Mao Aihua , Liu Jingwei , Li Pengjie , Zheng Shaoqin , Tong Tong , Li Zexu , Zhang Haijiao , Ma Lanjing , Lin Jiahui , Pang Zhongqiu , Han Qing , Qi Fukang , Zhang Xinjun , Chen Maorong , He Xi , Zhang Xi , Fei Teng , Liu Bi-Feng , Gao Daming , Cao Liu , Wang Qiang , Li Yiwei , Sheng Ren 
Published: November 2022

AbstractWnt/β-catenin signaling is a conserved pathway crucially governing development, homeostasis and oncogenesis. Discovery of novel regulators holds great values in both basic and translational research. Through screening, we identified a deubiquitinase (DUB) USP10 as a novel and critical modulator of β-catenin. Mechanistically, USP10 binds to key scaffold Axin1 via conserved motifs and stabilizes Axin1 through K48-linked deubiquitination, and surprisingly, tethers Axin1 and β-catenin physically while promoting phase separation for β-catenin suppression regardless of its enzymatic activity. Functionally, USP10 prominently regulates embryonic development and intestinal homeostasis by antagonizing β-catenin via DUB activity. In colorectal cancer, USP10 substantially represses cancer growth mainly through physical binding compensation and phase separation promotion and correlates with Wnt/β-catenin magnitude clinically. Collectively, we discovered USP10 functioning in multiple biological processes against β-catenin and unearthed a novel enzyme-dependent and -independent “dual-regulating” mechanism by which USP10 utilizes parallelly and context-dependently. USP10 inhibitor was suggested in treating certain Wnt-related diseases.