bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and Morphological Identification of Fish gut Micro biota at different growth stages of Labeo rohita
Authors: mazhar muhammad waqar 
Published: May 2022

Abstract
Introduction:The gut microbiota plays a significant role in fish health and pathogenicity. L.rohita is well known farmed fish it is also a culture with other major carps.Methodology:The present study was conducted to determine the molecular and morphological identification of gut microbiota of L.rohita collected from the fish hatchery in Faisalabad. Isolation of bacteria was carried out through the culture techniques. The morphological identification of bacterial isolates was determined by the gram staining, biochemical tests TSI, and MR-VP test. The molecular identification was done by 16S rRNA techniques.Results:The results of the present research work showed that the 20 strains of bacteria were isolated from the gut of L.rohita and all these isolates were gram-negative and morphological identify based on biochemical tests i-e TSI and MR-VP test. Biofilm results indicated that six, eight, and six isolates showed weak, moderate, and strong biofilm.Conclusion:The highest and lowest resistance showed against cefadroxil and Levofloxin respectively. The lowest intermediate isolates were found against cefadroxil, polymyxin B, and colistin, and the highest intermediate was found against ceftriaxone. The highest sensitive isolates were found against Levofloxin and the lowest sensitive isolate was found in cefadroxil, nitrofurantoin, and cefoxitin. Based on 16Sr RNA sequencing and phylogenetic relationship among isolates 17 isolates were determined in the present study. Klebsiella pneumonia, Enterobacter cloacae, Pseudomonas oleovorans, Morganella morganii, Citrobacter freundii, Proteus mirabilis, Citrobacter braakii, Enterobacter hormaechei, Psychrobacter sanguinis, Shigella dysenteriae, Citrobacter cronae, Shigella sonnei, Pseudomonas sihuiensis, Proteus mirabilis, Enterobacter hormaechei were found in the intestine of L.rohita
Using Tensor Contractions to Derive the Structure of Slice-Wise Multiplications of Tensors with Applications to Space-Time Khatri-Rao coding for MIMO-OFDM Systems
Authors: Sokal Bruno , Naskovska Kristina , de Almeida André Lima Férrer , Haardt Martin 
Published: May 2022

Abstract
The slice-wise multiplication of two tensors is required in a variety of tensor decompositions (including PARAFAC2 and PARATUCK2 ) and is encountered in many applications, including the analysis of multidimensional biomedical data (EEG, MEG, etc. ) or multi-carrier MIMO systems. In this paper, we propose a new tensor representation that is not based on a slice-wise (matrix) description, but can be represented by a double contraction of two tensors. Such a double contraction of two tensors can be efficiently calculated via generalized unfoldings. It leads to new tensor models of the investigated system that do not depend on the chosen unfolding (in contrast to matrix models) and reveal the tensor structure of the data model, such that all possible unfoldings can be seen at the same time. As an example, we apply this new concept to the design of new receivers for multi-carrier MIMO systems in wireless communications. In particular, we consider MIMO-OFDM systems with and without Khatri-Rao coding. The proposed receivers exploit the channel correlation between adjacent subcarriers , require the same amount of training symbols as traditional OFDM techniques, but have an improved performance in terms of the symbol error rate. Furthermore, we show that the spectral efficiency of the Khatri-Rao coded MIMO-OFDM can be increased by introducing cross- coding such that the ''coding matrix" also contains useful information symbols. Considering this transmission technique, we derive a tensor model and two types of receivers for cross- coded MIMO-OFDM systems using the double contraction of two tensors.
Local Excision versus Total Mesorectal Excision after favourable response to neoadjuvant therapy in rectal cancer: a multi-centre experience
Authors: Fareed Ahmed M , Shahatto Fayz , Khater Ashraf , Kotb Sherif Z. , Elzahaby Islam A , Eldamshety Osama , Khan Jim S. 
Published: May 2022

Abstract
Introduction
The gold standard surgical management of curable rectal cancer is proctectomy with total mesorectal excision. Adding preoperative radiotherapy improved local control. The promising results of neoadjuvant chemoradiotherapy raised the hopes for conservative, yet oncologically safe management, probably using local excision technique.
Patients and Methods
This study is a prospective comparative phase III study, where 46 rectal cancer patients were recruited from patients attending Oncology Centre of Mansoura University and Queen Alexandra Hospital Portsmouth University Hospital NHS with a median follow-up 36 months. The two recruited groups were as follows: Group (A): 18 patients who underwent conventional Radical Surgery by TME. Group (B): 28 patients who underwent Trans-anal Endoscopic Local Excision. Patients of resectable low rectal cancer (below 10 cms from anal verge) with sphincter saving procedures were included: cT1-T3N0.
Results
The median operative time for LE was 120 minutes versus 300 in TME (p < 0.001), median blood loss was 20 ml versus 100 ml in LE and TME respectively (p < 0.001). Median hospital stay was 3.5 days versus 6.5 days (p = 0.009). No statistically significant difference in median DFS (64.2 months for LE versus 63.2 months for TME, p = 0.85) and median OS (72.9 months for LE versus 76.3 months for TME, p = 0.43). No statistically significant difference in LARS scores and QoL was observed between LE and TME (p = 0.798, p = 0.799).
Conclusion
LE seems a good alternative to radical rectal resection in carefully selected responders to neoadjuvant therapy after thorough pre-operative evaluation, planning and patient counselling.
Topological Interface-state Lasing in a Polymer-Cholesteric Liquid Crystal Superlattice
Authors: Zhang Xinzheng , Wang Yu , Yang Donghao , Gao Shaohua , Drevensek-Olenik Irena , Wu Qiang , Chemingui Marouen , Chen Zhigang , Xu Jingjun 
Published: May 2022

Abstract
The advance of topological photonics has heralded a revolution for manipulating light as well as for the development of novel photonic devices such as topological insulator lasers. Here, we demonstrate topological lasing of circular polarization in a polymer-cholesteric liquid crystal (P-CLC) superlattice, tunable in the visible wavelength regime. By use of the femtosecond-laser direct-writing and self-assembling techniques, we establish the P-CLC superlattice with a controlled mini-band structure and a topological interface defect, thereby achieving a low threshold for robust topological lasing at about 0.4 μJ. Thanks to the chiral liquid crystal, not only the emission wavelength is thermally tuned, but the circularly polarized lasing is readily achieved. Our results bring about the possibility to realize compact and integrated topological photonic devices at low cost, as well as to engineer an ideal platform for exploring topological physics that involves light-matter interaction in soft-matter environments.
Associations of intracranial atherosclerotic burden and imaging-based lesion category with ischemic stroke
Authors: Hu Lele , Peng Zhongyong , Pan Yuesong , Gong Ling , Liu Yuanliang , Mi Donghua , Sun Ping 
Published: May 2022

Abstract
Background
Intracranial atherosclerotic stroke (ICAS) is strongly associated with single and multiple infarctions. The present study aimed to assess the associations of imaging-based lesion category and intracranial atherosclerosis burden (ICASB) with ischemic stroke (IS), and to determine whether multiple infarctions may be related to increased prevalence of ICASB.
Methods
In this study, 2864 consecutive cases from 22 Chinese hospitals who had acute cerebral ischemia < 7 days after symptom onset were included. After magnetic resonance angiography, the patients were categorized into three groups, with scores of < 4, 4–7 and > 7, respectively, according to the degree of IS reflected by ICASB, and also divided into single and multiple infarction groups taking into consideration the number of acute infarction-related lesions.
Results
Reduced NIHSS score at patient admission and complete circle of Willis were closely associated with single infarction. However, patients with multiple infarctions had higher ICASB compared with the single infarction group, particularly in the ICASB4-7 and > 7 subgroups (P < 0.0002). Patients with multiple infarctions showed prolonged hospital stay (p < 0.0033) and increased brain damage or ischemic stroke (IS) recurrence within 1 year (p < 0.0008). In multivariate analysis, complete circle of Willis (HR = 2.56, 95%CI 1.16–5.66; P = 0.0199) and family history of stroke (HR = 2.07, 95%CI 1.054–4.071; P = 0.034) were independent predictors of multiple infarctions, unlike elevated ICASB (ICASB4-7 group, p = 0.23; ICASB > 7group, p = 0.24)
Conclusions
Patients with multiple infarctions have significantly elevated ICASB, and show increase brain damage or IS recurrence within 1 year. High ICASB is not an independent predictor of multiple infarctions.
Early differentiation of neurodegenerative diseases using the novel QSM technique: What is the biomarker of each disorder?
Authors: Nikparast Farzaneh , Ganji Zohreh , Zare Hoda 
Published: May 2022

Abstract
During neurodegenerative diseases, the brain undergoes morphological and pathological changes; Iron deposits are one of the causes of pathological changes in the brain.The Quantitative susceptibility mapping (QSM) technique, a type of magnetic resonance (MR) image reconstruction, is one of the newest diagnostic methods for iron deposits to detect changes in magnetic susceptibility. Numerous research projects have been conducted in this field. The purpose of writing this review article is to identify the first deep brain nuclei that undergo magnetic susceptibility changes during neurodegenerative diseases such as Alzheimer's or Parkinson's disease. The purpose of this article is to identify the brain nuclei that are prone to iron deposition in any specific disorder.In addition to the mentioned purpose, this paper proposes the optimal scan parameters and appropriate algorithms of each QSM reconstruction step by reviewing the results of different articles. As a result, The QSM technique can identify nuclei exposed to iron deposition in various neurodegenerative diseases. Also, the selection of scan parameters is different based on the sequence and purpose; an example of the parameters is placed in the tables. The BET toolbox in FSL, Laplacian-based phase-unwrapping process, the V_SHARP algorithm, and morphology-enabled dipole inversion (MEDI) method are the most widely used algorithms in various stages of QSM reconstruction.
Long-term upper-extremity prosthetic control using Regenerative Peripheral Nerve Interfaces
Authors: Vu Philip , Vaskov Alex , Lee Christina , Jillala Ritvik , Davis Alicia , Kung Theodore , Kemp Stephen , Gates Deanna , Chestek Cynthia , Cederna Paul 
Published: May 2022

Abstract
Extracting motor signals directly from the peripheral nervous system poses challenges in obtaining both high amplitude and sustainable signals for upper-limb neuroprosthetic control. To translate peripheral nerve interfaces into the clinical space, these interfaces must provide consistent signals and prosthetic performance. Previously, we have demonstrated that the Regenerative Peripheral Nerve Interface (RPNI) is a biologically stable, bioamplifier of efferent motor action potentials. Here, we assessed the reliability of the RPNI signals in humans for long-term prosthetic control. RPNI signal quality, measured as signal-to-noise ratio, remained greater than 15 for up to 276 and 1054 days in participant 1 (P1), and participant 2 (P2), respectively. Though signal amplitude varied between sessions, P2 maintained prosthetic performance above 94% accuracy for 604 days without recalibration. Additionally, P2 completed a real-world multi-sequence coffee task with 99% accuracy 611 days without recalibration, demonstrating the potential of RPNIs as a long-term interface for enhanced prosthetic control.
Association between Genetic Risk Score and Tri-ponderal Mass Index Growth Trajectories among Different Dietary Consumption Adolescents in a Prospective Taiwanese Cohort
Authors: Wu Yi-Fan , Chien Kuo-Liong , Chen Yang-Ching 
Published: May 2022

Abstract
Background: Single-nucleotide polymorphisms (SNPs) in various genetic loci are associated with childhood obesity; however, their influence on adolescent growth patterns has rarely been explored. This study investigated whether genetic variants could predict tri-ponderal mass index (TMI)-derived growth trajectories and the interaction between genetic and dietary factors.Methods: We conducted Taiwan Puberty Longitudinal Study, a prospective cohort that recruited 1,135 children since 2018. Anthropometric measurements were recorded every three months, while dietary nutrition assessment and biological sampling for genotyping were collected during the first visit. TMI growth trajectory groups were identified using growth mixture modeling. A multinomial logistic regression model for different growth trajectories was used to examine the effect of candidate SNPs, and the most related SNPs were used to establish the genetic risk score. We then explored the effect of the genetic risk score in subgroup analysis according to dietary calories and different dietary consumption patterns.Results: Three TMI-based growth trajectory groups were identified among adolescents. The “increased weight” trajectory group accounted for approximately 9.7% of the participants. FTO/rs7206790 was associated with the increased weight growth trajectory after adjusting for the baseline TMI and other correlated covariates (odds ratio: 2.13, 95% confidence interval: 1.08–4.21). We generated the genetic risk score by using 4 SNPs (FTO/rs7206790, ADCY9/rs2531995, TFAP2B/rs4715210, and TMEM18/rs6548238) and selected the threshold of 10 points to define risk categories. There were 11.66% and 3.24% of participants belonged to the increased weight trajectory in high- and low-risk groups, respectively; and the predictive ability of the genetic risk score was notable among low calories intake participants (odds ratio: 1.90, 95% confidence interval: 1.18-3.05 vs. odds ratio: 1.17, 95% confidence interval: 0.78-1.75 in high calories intake group).Conclusion: Our results offer a new perspective on the genetic and dietary basis of changes in adolescent obesity status. Individualized interventions for obesity prevention may be considered among high-risk children.
Warming up for a better fever: a feasibility study in pediatric oncology
Authors: Krafft Hanno S , Raak Christa , Jenetzky Ekkehart , Zuzak Tycho J , Längler Alfred , Martin David D 
Published: May 2022

Abstract
Background
Fever in children is a major problem in pediatric oncology. Usual management leads to immediate antibiotic and antipyretic therapy, although there is consensus that antipyretic therapy should not be utilized to reduce body temperature. Increased body temperature during fever appears to be an effective modifier in terms of viral replication and enhanced host defense mechanisms against pathogens. Therefore, it might be beneficial to support febrile patients by applying gentle heat during the onset of fever to help the body to reach its new thermoregulatory set point.
Methods
A randomized, controlled, feasibility study over six months will be conducted in a pediatric oncology department in an academic hospital in Germany, in preparation for a multicenter clinical trial with two parallel groups concerning the efficacy of heat application vs. treatment as usual. Inclusion criteria are body temperatures ≥ 38.5°C in n = 24 cases of patients receiving chemotherapy aged 3 to 17 years. The first intervention consists of gentle heat application with electric blankets, hot water bottles or microwave heating pads at any sign of illness and onset of fever, with the aim of achieving a warm periphery equilibrated to trunk temperature of less than 0.5°C. The second intervention is the avoidance of antipyretics. The control group receives the standard antipyretic treatment of the participating hospital. Purpose of this feasibility study is proof of principle of intervention, evaluation of safety, feasibility, definition of endpoints and to receive basic data for sample size calculation and needed resources.
Discussion
Main goal is to improve the care of children with cancer by providing the best possible support for febrile episodes. If fever support by heat reduces discomfort, administration of antipyretics and maybe even antibiotics, this would be an advancement in oncological fever management. This feasibility study is intended to provide a basis for a main, multicenter, randomized trial, and demonstrate practicability of heat application in febrile patients in pediatric oncology.
Registration:
German Clinical Trials Register (DRKS), ID: DRKS00028273. Registered 14 April 2022. https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00028273Keywords: fever, heat application, antipyretics, pediatric oncology, feasibility study, study protocol
Comparative genomics and phylogenetic analysis of the chloroplast genomes in three medicinal Salvia species for bioexploration
Authors: Du Qing , ZENG Jing , Wang Liqiang , Chen Zhuoer , Zhou Junchen , Sun SIHUI , WANG BIN , LIU CHANG 
Published: May 2022

Abstract
Background: Salvia bowleyana, S. splendens, and S. officinalis are globally distributed and have been widely used to treat coronary heart disease, liver tumors, and viral diseases. To systematically determine their phylogenetic relationship and develop molecular markers for species determination, we sequenced and assembled their chloroplast genomes, and analyzed the genome characteristics. Moreover, we compared the phylogenetic specification and divergence genes fragments of chloroplast genomes from the three Salvia species. Results: The length of S. bowleyana, S. splendens, and S. officinalis chloroplast genomes were 151387 bp, 150604 bp, and 151163 bp, respectively. The sizes of the large-single copy, small-single copy, and inverted repeat regions were 82772 bp, 17573 bp, and 51042 bp for S. bowleyana; 82181 bp, 17857 bp, and 50566 bp for S. splendens; 82429 bp, 17510 bp, and 51224 bp for S. officinalis, respectively. The GC contents of the three chloroplast genomes were 38.01%, 38.04%, 38.04%, partly. In the comparison of chloroplast genomes from Lamiaceae family, the six genes ndhB, rpl2, rpl23, rps7, rps12, and ycf2 were present in the IRs regions of all 41 chloroplast genomes in the Lamiaceae family. We found that one of the gene rpl20 was intact and stably occurred in all 41 species chloroplast genomes, however, another pseudogene one was lost in that of 40 species except the Dracocephalum heterophyllum. For the repeat analysis, 29 tandem repeats, 35, 29, 24 simple-sequence repeats(SSRs), and 47, 49, 40 interspersed repeats were identified in the three Salvia species chloroplast genomes based on the diverse requirements. The three specific intergenic sequences(IGS) of rps16-trnQ-UUG, trnL-UAA-trnF-GAA, and trnM-CAU-atpE were found to discriminate the certain species by comparing 23 Salvia chloroplast genomes. Six genes including rpl22, rps19, rpl2, ycf1, ndhF, and psbA were found in the highly diverse IR boundary regions. The genetic distance analysis of IGS showed the trnL-UAG-ccsA, rps16-trnQ-UUG, ccsA-ndhD, rps15-ycf1, and ndhE-ndhG regions had the higher variability. Furthermore, the phylogenetic tree inferred that the 23 Salvia species formed a monophyletic group. Lastly, two pairs of Genus-specific DNA barcode primers were identified, which can be used to amplify the part sequence of trnM-CAU-atpE and ccsA-ndhD region. Conclusions: We acquired the complete chloroplast genome of the three Salvia species, which will provide a solid foundation to understand their phylogenetic status in Salvia genus. Moreover, the research can provide the probability to discriminate the Salvia species compared with the genomics between the phenotype and the distinction of gene fragments .
Meta-analysis of the effect of irbesartan combined with different drugs on the efficacy and function of male patients with hypertension
Authors: Geng ZhiXin , Miao Feng , Gou Licheng , Gao Feng , Dong Taiwei , Li Min , Bai Yang , Xi miaomiao , Wei peifeng 
Published: May 2022

Abstract
Objective A systematic review of the clinical efficacy of irbesartan combined therapy in the treatment of hypertension and erectile function in men. Methods Use computer to search databases such as CNKI, CBM, Weipu, Wanfang, Embase, PubMed and Cochrane, and find all the randomized controlled trials (RCT) about the effect of irbesartan combination on the sexual function of male hypertensive patients. After screening according to the inclusion and exclusion criteria, the Cochrane risk of bias tool was used to evaluate the methodological quality of the included studies,and use Review Manager 5.2 software for system evaluation. Results Finally, 11 eligible RCTs with a total of 1350 participants were screened, and a meta-analysis was completed based on these RCTs. The results of Meta analysis showed that compared with other combination medication groups,Irbesartan combination group was better than other medication groups in the incidence of ED (OR = 0.57, 95％CI=(0.37,0.86), P=0.008), serum T (MD = 1.27, 95％CI=(0.69,1.84), P<0.0001), and SHBG (MD = -0.10, 95％CI=(-0.16,-0.04), P=0.002). There was no statistical difference in the total effective rate of lowering blood pressure, the rate of lowering blood pressure, and erectile function. None of the included RCTs mentioned serious adverse events. Conclusions The combination of irbesartan in the treatment of male hypertensive patients with sexual dysfunction was better than other combination groups, and there were no serious adverse reactions during the treatment. However, due to the generally low quality of the included studies, so a larger sample, multi-center, high-quality clinical trial was needed to prove it.
Synthesis and Comparative Evaluation of 177 Lu-labeled PEG and non-PEG variant peptides as HER2-targeting probes
Authors: Sharma Amit Kumar , Sharma Rohit , Vats Kusum , Sarma Haladhar Dev , Mukherjee Archana , Das Tapas , Satpati Drishty 
Published: May 2022

Abstract
Highest global cancer incidence of female breast cancer is a matter of great concern. HER2-positive breast cancers have high mortality rate hence detection at an early stage is vital for successful treatment, improved cancer care and survival rate. Radiolabeled peptides have emerged as new alternatives to radiolabeled antibodies to overcome the limitations of slow clearance and uptake in non-target tissues. Herein, DOTA-A9 peptide and its pegylated variant were constructed on solid phase and radiolabeled with 177LuCl3. 177Lu-DOTA-A9 and 177Lu-DOTA-PEG4-A9 displayed high binding affinity (Kd = 48.4 ± 1.4 and 55.7 ± 12.3 nM respectively) in human breast carcinoma SKBR3 cells. Two radiopeptides exhibited renal excretion and rapid clearance from normal organs. Uptake in SKBR3 tumor and tumor-to-background ratios were significantly higher (p < 0.05) for 177Lu-DOTA-PEG4-A9 at the three time points investigated. Two radiopeptides could clearly visualize xenografts in SPECT images at 3, 24 and 48 h p.i. indicating their potential for further exploration as HER2-targeting probes. The encouraging in vivo profile of PEG construct, 177Lu-DOTA-PEG4-A9 incentivizes future studies for clinical applications.