bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact on quality of life of an intervention providing additional information to patients with allergic contact dermatitis; a randomized clinical trial
Authors: Mossing K. , Dizdarevic A. , Svensson Å. , Sonesson A. 
Published: July 2022

AbstractBackgroundAllergic contact dermatitis can negatively impact an individual's daily life in terms of work and interpersonal relationships. Patch‐tested individuals show an improved quality of life (QoL).ObjectivesWe aimed to assess the impact on QoL after patch testing and what value an intervention would have on QoL.MethodsDermatology Quality of Life Index (DLQI) were assessed in participants with positive patch test reaction. The participants were randomized, in parallel design, into two groups that received either standard information (controls, n = 70) or a reminder letter in addition to standard information (intervention group, n = 66), ClinicalTrials.gov NCT01953380.ResultsThe response rate was 74% (n = 136). The DLQI score was significantly lower 1 year after patch testing in comparison with baseline in the entire group (mean DLQI 6.3 and 4.5 respectively, 95% CI 0.93–2.72, P < 0.001). However, linear regression analyses showed no significant differences in DLQI score at follow‐up between the intervention and control groups. Neither age nor gender had impact on DLQI score.ConclusionThere was an improvement of QoL at follow‐up in the entire group. However, the intervention performed did not show any significantly greater improvement concerning QoL. Further research is needed to understand what factors apart from patch testing and medical care may affect QoL in patients with contact dermatitis, and what interventions are needed to improve QoL.
Genome‐wide association study of dermatophytosis in the UK Biobank cohort
Authors: Lehrer S. , Rheinstein P.H. 
Published: July 2022

AbstractBackgroundAnalyses of the hereditary propensity to dermatophytosis have revealed several proven genetic relationships. They include CARD9 deficiency, HLA‐DR4 and HLA‐DR8 type and genes encoding interleukin‐22, defensin 2 and 4, and genetic defects in dectin‐1, which increased the prevalence of dermatophytosis in families and were involved in the inheritance of susceptibility in their members.MethodsTo further investigate the genetic basis of dermatophytosis, we performed a genome‐wide association study (GWAS) of the UK Biobank cohort. To identify cases of dermatophytosis, we used ICD10 code B35, which covers Tinea barbae, Tinea capitis, Tinea unguium, Tinea manuum, Tinea pedis, Tinea corporis, Tinea imbricata, Tinea cruris, other dermatophytoses and dermatophytosis, unspecified. Data processing was performed on Minerva, a Linux mainframe with Centos 7.6, at the Icahn School of Medicine at Mount Sinai. We used PLINK, a whole‐genome association analysis toolset, to analyse the UKB chromosome files and the UK Biobank Data Parser (ukbb parser), a python‐based package that allows easy interfacing with the large UK Biobank dataset. We used LocusZoom for the Manhattan and q‐q plots. Other statistical analyses were done with R and SPSS 25.ResultsGenome‐wide association study (GWAS) and meta‐analysis association statistics highlighted one susceptibility locus, Tubulointerstitial Nephritis Antigen (TINAG), with genome‐wide significance for dermatophytosis. The top SNP was rs16885197, a missense variant within TINAG, position chr6:54308557, alleles A > G, minor allele frequency (MAF) 0.014. Multivariate logistic regression indicated that the minor G allele increased odds ratio of dermatophytosis by 7.8. Carrying two G alleles raised dermatophytosis odds ratio by a factor of 14.ConclusionMore research into genetic and other predisposing factors for dermatophytosis is critical because of the implications for prophylaxis and therapy. It might be possible to prevent infection and recurrence by identifying people who are vulnerable to chronic dermatophytosis. Identifying high‐risk families would enable their members to be educated about the dangers of fungal diseases. New therapeutic techniques to target altered hormonal and immune response pathways might be created. TINAG is a prospective target that should be investigated, based on the findings of this article.
Serum autoantibody reactivity in bullous pemphigoid is associated with neuropsychiatric disorders and the use of antidiabetics and antipsychotics: a large, prospective cohort study
Authors: Dikmen H.O. , Yilmaz K. , Benoit S. , Bernard P. , Drenovska K. , Gerdes S. , Gläser R. , Günther C. , Homey B. , Horváth O.N. , Huilaja L. , Joly P. , Kiritsi D. , Meller S. , Patsatsi A. , Sárdy M. , Schauer F. , Shahid M. , Sticherling M. , Tasanen K. , Vassileva S. , Worm M. , Zillikens D. , Sadik C.D. , van Beek N. , König I.R. , Schmidt E. 
Published: July 2022

AbstractBackgroundBullous pemphigoid (BP), the by far most frequent autoimmune blistering skin disease (AIBD), is immunopathologically characterized by autoantibodies against the two hemidesmosomal proteins BP180 (collagen type XVII) and BP230 (BPAG1 or dystonin). Several comorbidities and potentially disease‐inducing medication have been described in BP, yet a systematic analysis of these clinically relevant findings and autoantibody reactivities has not been performed.ObjectiveTo determine associations of autoantibody reactivities with comorbidities and concomitant medication.MethodsIn this prospective multicenter study, 499 patients diagnosed with BP in 16 European referral centers were included. The relation between anti‐BP180 NC16A and anti‐BP230 IgG ELISA values at the time of diagnosis as well as comorbidities and concomitant medication collected by a standardized form were analysed.ResultsAn association between higher serum anti‐BP180 reactivity and neuropsychiatric but not atopic and metabolic disorders was observed as well as with the use of insulin or antipsychotics but not with dipeptidyl peptidase‐4 (DPP4) inhibitors, inhibitors of platelet aggregation and L‐thyroxine. The use of DPP4 inhibitors was associated with less anti‐BP180 and anti‐BP230 reactivity compared with BP patients without these drugs. This finding was even more pronounced when compared with diabetic BP patients without DPP4 inhibitors. Associations between anti‐BP180 and anti‐BP230 reactivities were also found in patients using insulin and antipsychotics, respectively, compared with patients without this medication, but not for the use of inhibitors of platelet aggregation, and L‐thyroxine.ConclusionTaken together, these data imply a relation between autoantibody reactivities at the time of diagnosis and both neuropsychiatric comorbidities as well as distinct concomitant medication suggesting a link between the pathological immune mechanisms and clinical conditions that precede the clinically overt AIBD.
Strategies to engage family physicians in primary care research: A systematic review
Authors: Girard Ariane , Dugas Michèle , Lépine Johanie , Carnovale Valérie , Jalbert Laura , Turmel Audrey , Stéfan Théo , Poirier Andrée‐Anne , Mailhot Benoit , Skidmore Becky , Couturier Yves , Miller Shandi , LeBlanc Annie 
Published: July 2022

AbstractRationaleMoving towards high quality primary health care, involving family physicians in primary care research becomes an essential prerequisite to ensures a better adoption and routinization of patient‐centred, evidence‐based practices.AimTo assess the effectiveness of strategies to engage family physicians in primary care research.MethodsWe systematically reviewed evidence for strategies used to engage family physicians in primary care research. We included any study design that reported at least one quantitative outcome. Searches were carried out on MEDLINE, Embase, PsycINFO and Web of Science. Pairs of reviewers independently screened for publications in two stages using standardized forms. We performed data analysis through a narrative synthesis approach, using the Reasoned‐action approach as framework.ResultsA total of 4859 deduped records were identified of which 41 studies met the eligibility criteria and were included for analysis. The majority of studies (n = 35) investigated family physician's participation in a research project. They aimed to influence family physicians' intention (n = 7) or their ability (n = 3) to participate in a research project. Three types of strategies (compensation/incentive, recruitment by a peer and support from a research network or an academic institution) demonstrated a significant increase in participation rate. Methodological quality of the studies evaluating these strategies was relatively low. Few studies (n = 6) targeted research capacity‐building programmes with no significant impact noted.ConclusionNumerous strategies have been used to engage family physicians in primary care research, but few studies evaluated their effectiveness in a rigorous way.RegistrationThe protocol of this review was registered with the SPOR Evidence Alliance and on the PROSPERO platform (registration number: CRD42020189322).
The RECEPTOR‐LIKE PROTEIN53 immune complex associates with LLG1 to positively regulate plant immunity
Authors: Chen Renjie , Sun Pengwei , Zhong Guitao , Wang Wei , Tang Dingzhong 
Published: July 2022

AbstractPattern recognition receptors (PRRs) sense ligands in pattern‐triggered immunity (PTI). Plant PRRs include numerous receptor‐like proteins (RLPs), but many RLPs remain functionally uncharacterized. Here, we examine an Arabidopsis thaliana RLP, RLP53, which positively regulates immune signaling. Our forward genetic screen for suppressors of enhanced disease resistance1 (edr1) identified a point mutation in RLP53 that fully suppresses disease resistance and mildew‐induced cell death in edr1 mutants. The rlp53 mutants showed enhanced susceptibility to virulent pathogens, including fungi, oomycetes, and bacteria, indicating that RLP53 is important for plant immunity. The ectodomain of RLP53 contains leucine‐rich repeat (LRR) motifs. RLP53 constitutively associates with the LRR receptor‐like kinase SUPPRESSOR OF BRASSINOSTEROID INSENSITIVE1‐ASSOCIATED KINASE (BAK1)‐INTERACTING RECEPTOR KINASE1 (SOBIR1) and interacts with the co‐receptor BAK1 in a pathogen‐induced manner. The double mutation sobir1‐12 bak1‐5 suppresses edr1‐mediated disease resistance, suggesting that EDR1 negatively regulates PTI modulated by the RLP53–SOBIR1–BAK1 complex. Moreover, the glycosylphosphatidylinositol (GPI)‐anchored protein LORELEI‐LIKE GPI‐ANCHORED PROTEIN1 (LLG1) interacts with RLP53 and mediates RLP53 accumulation in the plasma membrane. We thus uncovered the role of a novel RLP and its associated immune complex in plant defense responses and revealed a potential new mechanism underlying regulation of RLP immune function by a GPI‐anchored protein.
Starch phosphorylase 2 is essential for cellular carbohydrate partitioning in maize
Authors: Qin Yao , Xiao Ziyi , Zhao Hailiang , Wang Jing , Wang Yuanru , Qiu Fazhan 
Published: July 2022

AbstractCarbohydrate partitioning is essential for plant growth and development, and its hindrance will result in excess accumulation of carbohydrates in source tissues. Most of the related mutants in maize (Zea mays L.) display impaired whole‐plant sucrose transport, but other mechanisms affecting carbohydrate partitioning have seldom been reported. Here, we characterized chlorotic leaf3 (chl3), a recessive mutation causing leaf chlorosis with starch accumulation excessively in bundle sheath chloroplasts, suggesting that chl3 is defective in carbohydrate partitioning. Positional cloning revealed that the chl3 phenotype results from a frameshift mutation in ZmPHOH, which encodes starch phosphorylase 2. Two mutants in ZmPHOH exhibited the same phenotype as chl3, and both alleles failed to complement the chl3 mutant phenotype in an allelism test. Inactivation of ZmPHOH in chl3 leaves reduced the efficiency of transitory starch conversion, resulting in increased leaf starch contents and altered carbohydrate metabolism patterns. RNA‐seq revealed the transcriptional downregulation of genes related to photosynthesis and carbohydrate metabolism in chl3 leaves compared to the wild type. Our results demonstrate that transitory starch remobilization is very important for cellular carbohydrate partitioning in maize, in which ZmPHOH plays an indispensable role.
Centromere protein J is overexpressed in human glioblastoma and promotes cell proliferation and migration
Authors: de Freitas Gabriella P. A. , Geraldo Luiz Henrique M. , Faria Bruna M. , Alves‐Leon Soniza Vieira , de Souza Jorge Marcondes , Moura‐Neto Vivaldo , Pontes Bruno , Romão Luciana F. , Garcez Patrícia P. 
Published: July 2022

AbstractGlioblastoma is the most common and malignant type of primary brain tumor. Previous studies have shown that alterations in centrosome amplification and its components are frequently found in treatment‐resistant tumors and may be associated with tumor progression. A centrosome protein essential for centrosome biogenesis is the centromere protein J (CENPJ), known to control the proliferation of neural progenitors and hepatocarcinoma cells, and also neuronal migration. However, it remains unknown the role of CENPJ in glioblastoma. Here we show that CENPJ is overexpressed in human glioblastoma cell lines in comparison to human astrocytes. Using bioinformatics analysis, we find that high Cenpj expression is associated with poor prognosis in glioma patients. Examining Cenpj loss of function in glioblastoma by siRNA transfection, we find impairments in cell proliferation and migration. Using a Cenpj mutant version with the deleted PN2‐3 or TCP domain, we found that a conserved PN2‐3 region is required for glioblastoma migration. Moreover, Cenpj downregulation modulates glioblastoma morphology resulting in microtubules stabilization and actin filaments depolymerization. Altogether, our findings indicate that CENPJ controls relevant aspects of glioblastoma progression and might be a target for therapeutic intervention and a biomarker for glioma malignancy.image
Vaping and dynamic risk construction: Toward a model of adolescentrisk‐relatedschema development
Authors: Tanner Emily C. , Tanner John F. , Vizcaino Franklin Velasco , Yang Zhiyong 
Published: July 2022

AbstractAdolescents experience vulnerability because their brains are not fully developed and they have limited knowledge and experience, inhibiting their ability to assess and mitigate risks. Drawing on literature from different disciplines, this paper develops a model that illustrates the dynamic nature of information and experience acquisition and ongoing risk and benefit assessment. New concepts are created to reflect better adolescent risk‐related schema:fungibility,fog of vulnerability, andpoints of tension, all of which are useful in understanding how intentions and in‐the‐moment decisions are made in the context of vaping.
Tooth preservation vs. extraction and implant placement in periodontally compromised patients: A systematic review and analysis of studies
Authors: Sarafidou Katia , Lazaridi Ioanna , Gotsis Sotirios , Kirmanidou Yvoni , Vasilaki Dimitra , Hirayama Hiroshi , Michalakis Konstantinos 
Published: July 2022

AbstractPurposeThe aim of this systematic review was to identify studies with a minimum of 5‐years follow‐up, reporting on the management of periodontally compromised teeth with either extraction and subsequent implant placement or teeth preservation with conventional periodontal treatment and application of regenerative procedures. The outcomes of these two approaches, based on clinical and radiographic data and the incidence of tooth‐ and implant‐loss, were also investigated.Material and methodsA systematic search for studies reporting on clinical and radiographic outcomes of periodontal treatment or replacement of periodontally compromised teeth with implants was conducted in 3 electronic databases, followed by a hand‐search in 8 journals. Only randomized controlled trials (RCTs), cohort studies, and case series with prospective design were included.ResultsThe initial search resulted in 1080 papers. After the first two screenings, 24 publications were selected for inclusion in this systematic review. The treatment protocols for the teeth preservation group contained nonsurgical and/or surgical periodontal treatment with or without regeneration procedures. The implant studies included extraction of periodontally involved teeth and implant placement with or without bone and soft tissue augmentation, followed by restoration with fixed dental prostheses (FDPs). Survival rates ranged between 81.8% and 100% in the tooth retention group, and between 94.8% and 100% in the implant group. In the extraction group, no complications were reported for 76.09% of the implants. Similarly, no complications were reported for 86.83% of the tooth retention group. The lack of standardized comparable studies prohibited conduction of a metaanalysis.ConclusionBoth treatment approaches, treatment of periodontally compromised teeth, or tooth extraction followed by implant placement, present high survival rates. The application of bone regeneration techniques improves the long‐term prognosis of periodontally involved teeth. Hence, treatment of periodontally involved teeth with subsequent application of a rigorous maintenance protocol can be a viable alternative for a number of years, before proceeding to extraction and replacement with dental implants. More well‐designed randomized controlled trials are needed in order to draw definite conclusions on the subject.
PSH‐C: A measure of peer sexual harassment among children
Authors: Valik Andrea , Holmqvist Gattario Kristina , Lunde Carolina , Skoog Therése 
Published: July 2022

AbstractPeer sexual harassment among adolescents is a widespread problem worldwide, associated with several adverse outcomes. Although retrospective reports indicate that people's first experience of peer sexual harassment occurs before puberty, research in younger ages is still scarce and measures of peer sexual harassment developed for children are lacking. This study explores the possibility of measuring peer sexual harassment in late childhood by evaluating a new scale of developmentally informed items, the Peer Sexual Harassment Scale—Child (PSH‐C), designed to capture peer sexual harassment (i.e., victimization, perpetration, and witnessing) in late childhood (ages 10–12 years) in the school context. Self‐report surveys were distributed to 1007 fourth‐grade students in Sweden (M age = 10.0 years, SD = .3, n = 516 girls). The PSH‐C showed good psychometric properties, supporting the scale's ability to capture peer sexual harassment in late childhood. Exploratory structural equation analyses suggested a two‐factor structure which was confirmed by confirmatory factor analyses: 1) direct verbal sexual aggression, and 2) direct physical sexual harassment, verbal comments and jokes, and visual sexual harassment. The division of the structure contradicts previous studies of adolescents, possibly indicating a difference in the peer sexual harassment construct in relation to development and age.
Improving uncertainty tolerance in medical students: A scoping review
Authors: Patel Priya , Hancock Jason , Rogers Morwenna , Pollard Samuel R. 
Published: July 2022

AbstractIntroductionUncertainty is an inevitable part of medical practice. An ability to tolerate uncertainty is viewed as a key competency across many health‐care systems. Poor uncertainty tolerance (UT) has been linked to negative outcomes including reduced psychological well‐being in medical students. A variety of medical education interventions have been developed with the intention of increasing medical students' UT. However, there is no synthesis of these studies available to inform education and research practice. Our aim was to conduct a scoping review of medical education interventions that evaluate their impact on UT.MethodsMedline, PsycInfo, Embase and ERIC databases were searched for articles published from inception to December 2020. An extensive supplementary search was conducted and both quantitative and qualitative evaluations were included. For each intervention, we categorised the stimulus of uncertainty (ambiguity, complexity and/or probability) and mapped the students' reported cognitive, behavioural, and/or emotional response(s) to uncertainty onto an existing conceptual framework.ResultsTwenty‐two of 24 included studies reported a positive impact on medical student UT in at least one domain (cognitive, behavioural or emotional). Interventions included problem based learning‐based curricula, medical humanities, simulation, reflection and assessment. We found in four studies that a negative response in the emotional domain was reported despite positive responses also being reported in the cognitive and/or behavioural domains.ConclusionWe identified a range of medical education interventions which report a positive impact on medical student UT. Further research is required to understand why a single intervention may stimulate a negative emotional response alongside a positive cognitive or behavioural response. In turn, this could support stakeholders such as policymakers and institutions to adapt the medical curriculum to better prepare their medical students for practice by enhancing their UT.
Immunohistopathology of oral mucosal chronic graft‐versus‐host disease severity and duration
Authors: Tollemar Victor , Ström Jennifer , Tudzarovski Nikolce , Häbel Henrike , Legert Karin Garming , Heymann Robert , Warfvinge Gunnar , Le Blanc Katarina , Sugars Rachael Victoria 
Published: July 2022

AbstractObjectiveChronic graft‐versus‐host disease (cGVHD) is the main cause of late non‐relapse mortality following hematopoietic cell transplantation. Oral mucosal (om‐) cGVHD is common, but diagnosis and assessment rely on clinical interpretation and patient‐reported symptoms. We investigated immunohistopathological profiles with respect to om‐cGVHD severity disease duration.Material and methodsNinety‐four transplant patients and 15 healthy controls (n = 212 biopsies) were investigated by quantitative immunohistochemistry for T cells (CD4, CD8, and CD5), B cells (CD19 and CD20), macrophages (CD68), and Langerhans cells (CD1a).ResultsWe found significant increases in T (CD4, CD8) and monocytic (CD68) cells in om‐cGVHD, and a notable absence of B (CD19 and CD20) cells. Histopathological activity correlated with increased CD4, CD8 and CD68. However, CD4 was associated with mild om‐cGVHD, whereas CD8 and CD68 were found to be elevated in severe om‐cGVHD. CD8 and CD68 levels were raised at disease onset, but during late phase, the predominant CD68 population was accompanied by CD4.ConclusionOral cGVHD is a heterogenous clinical disorder, but our knowledge of the underlying biology remains limited. We highlight the importance of CD4, CD8 and CD68 immune profiling, together with histological grading for the staging of oral cGVHD, to broaden our understanding of the biology and individual disease course.
Relativism and Two Kinds of Branching Time
Authors: Ninan Dilip 
Published: July 2022

AbstractThis essay examines the case for relativism about future contingents in light of a distinction between two ways of interpreting the ‘branching time’ framework. The first step of the relativist argument is to argue for the ‘Non‐Determination Thesis’, the view that there is no unique actual future. The second step is to argue from the Non‐Determination Thesis to relativism. I show that first step of this argument fails. But despite that result, the second step is still of interest, because one might hold the Non‐Determination Thesis on alternative grounds. I then argue that whether the second step of the argument succeeds depends on how the branches in question are interpreted. If the branches are ersatz possible worlds, then the argument for relativism might go through. But if the branches are concrete parts of a ‘branching multiverse’, then the argument for relativism turns out to make implausible assumptions about the nature of personal identity over time.