bluuebunny/crossref_metadata_2025_split huggingface dataset:
Solid-state fermentation produces greater stearidonic acid levels in genetically engineered Mucor circinelloides
Authors: Xin F. , Wang R. , Chang Y. , Xie Z. , Zhao Y. , Zhang H. , Song Y. 
Published: September 2022

Abstract
Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) are important dietary components due to their health benefits and preventative role in cardiovascular disease. Fish-based and plant seed oils are rich in stearidonic acid (SDA; 18:4, n-3), which are readily metabolized into ω-3 PUFAs such as eicosapentaenoic acid. However, these natural sources of SDA are generally low yielding and are unlikely to meet global demands, so new sustainable microbial fermentative sources of SDA need to be identified. Expression of delta15-desaturase in the oleaginous filamentous fungus Mucor circinelloides (McD15D) has been used to construct a recombinant SDA-producing McD15D strain that produces 5·0% SDA levels using submerged fermentation conditions. Switching to solid-state fermentation conditions in the same medium with submerged fermentation resulted in this engineered strain producing significantly higher amounts of SDA. A Box–Behnken design of response surface methodology approach has been used to identify optimal glucose and ammonium tartrate concentrations and temperature levels to maximize SDA production. The use of these optimal solid-state fermentation conditions resulted in the spores and mycelium of the recombinant McD15D producing 19·5% (0·64 mg g−1) and 12·2% (1·52 mg g−1) SDA content, respectively, which represents an overall increase in SDA yield of 188·0% compared with SDA yields produced using submerged fermentation conditions.
Cognitive Continuum Theory: Can it contribute to the examination of confidentiality and risk‐actuated disclosure decisions of nurses practising in mental health?
Authors: Conlon Darren , Raeburn Toby , Wand Timothy 
Published: September 2022

AbstractNurses practising in mental health are faced with challenging decisions concerning confidentiality if a patient is deemed a potential risk to self or others, because releasing pertinent information pertaining to the patient may be necessary to circumvent harm. However, decisions to withhold or disclose confidential information that are inappropriately made may lead to adverse outcomes for stakeholders, including nurses and their patients. Nonetheless, there is a dearth of contemporary research literature to advise nurses in these circumstances. Cognitive Continuum Theory presents a single‐system intuitive‐analytical approach to examining and understanding nurse cognition, analogous to the recommended single‐system approach to decision‐making in mental health known as structured clinical judgement. Both approaches incorporate cognitive poles of wholly intuition and analysis and a dynamic continuum characterised by a ‘common sense’ blending of intuitive and analytical cognition, whereby cues presented to a decision‐maker for judgement tasks are weighed and assessed for relevance. Furthermore, Cognitive Continuum Theory promotes the importance of determining pattern recognition and functional relations strategies, which can be used to understand the operationalisation of nurse cognition.
A 90‐channel triaxial magnetoencephalography system using optically pumped magnetometers
Authors: Rea Molly , Boto Elena , Holmes Niall , Hill Ryan , Osborne James , Rhodes Natalie , Leggett James , Rier Lukas , Bowtell Richard , Shah Vishal , Brookes Matthew J. 
Published: September 2022

AbstractMagnetoencephalography (MEG) measures the small magnetic fields generated by current flow in neural networks, providing a noninvasive metric of brain function. MEG is well established as a powerful neuroscientific and clinical tool. However, current instrumentation is hampered by cumbersome cryogenic field‐sensing technologies. In contrast, MEG using optically pumped magnetometers (OPM‐MEG) employs small, lightweight, noncryogenic sensors that provide data with higher sensitivity and spatial resolution, a natural scanning environment (including participant movement), and adaptability to any age. However, OPM‐MEG is new and the optimum way to design a system is unknown. Here, we construct a novel, 90‐channel triaxial OPM‐MEG system and use it to map motor function during a naturalistic handwriting task. Results show that high‐precision magnetic field control reduced background fields to ∼200 pT, enabling free participant movement. Our triaxial array offered twice the total measured signal and better interference rejection compared to a conventional (single‐axis) design. We mapped neural oscillatory activity to the sensorimotor network, demonstrating significant differences in motor network activity and connectivity for left‐handed versus right‐handed handwriting. Repeatability across scans showed that we can map electrophysiological activity with an accuracy ∼4 mm. Overall, our study introduces a novel triaxial OPM‐MEG design and confirms its potential for high‐performance functional neuroimaging.
Histological characteristics of matrix metalloproteinase‐9 and tissue inhibitor of metalloproteinases‐1 in asthmatic murine model during A(H1N1)pdm09 infection
Authors: Kimura Sasagu , Yasudo Hiroki , Oga Atsunori , Fukano Reiji , Matsushige Takeshi , Hamano Hiroki , Hasegawa Hideki , Nakajima Noriko , Ainai Akira , Itoh Hiroshi , Shirabe Komei , Toda Shoichi , Atsuta Ryo , Hasegawa Shunji 
Published: September 2022

AbstractPandemic influenza virus A(H1N1)pdm09 infection occurred in healthy children and young adults, but asthmatic patients presented more rapid progression of respiratory distress and plastic bronchitis. To investigate the pathogenesis of worsening respiratory symptoms after A(H1N1)pdm09 infection, we focused on matrix metalloproteinase‐9 (MMP‐9) and tissue inhibitor of metalloproteinases‐1 (TIMP‐1). MMP‐9 and TIMP‐1 levels in bronchoalveolar lavage fluid and serum from mice with and without asthma were evaluated after A(H1N1)pdm09 or seasonal A(H1N1) infection. MMP‐9 levels were more elevated in Asthma/A(H1N1)pdm09‐infected mice than in non‐Asthma/A(H1N1)pdm09‐infected mice on both 3 and 7 days post‐infection. Immunohistochemical findings in this pneumonia model showed that MMP‐9 and TIMP‐1 positive cells were observed in blood vessels and bronchus of lung tissue in severe pathological findings of pneumonia with asthma. Microscopically, shedding cells and secretions were conspicuous in the trachea on days 3 and 7 post‐infection, in the A(H1N1)pdm09‐infected mice with asthma. Our results suggest that MMP‐9 and TIMP‐1 expressions are related to severe pneumonia in the A(H1N1)pdm09 infection with asthma, leading to cause epithelial cell shedding.
On the economic impacts of COVID‐19: A text mining literature analysis
Authors: Gonçalves Hugo S. , Moro Sérgio 
Published: September 2022

AbstractThe COVID‐19 outbreak has affected everyday lives worldwide. As governments started to implement confinement and business closure measures, the economic impact was felt by entire societies immediately. The urgency of such a theme has led researchers to study the phenomenon. Accordingly, the purpose of this research is to provide the state of the art on relevant dimensions and hot topics of research to understand the economic impacts of COVID‐19. In this survey, we conduct a text mining analysis of 301 articles published during 2020 which analyzed such economic impacts. By defining a set of relevant dimensions grounded on existing literature, we were able to extract a set of coherent topics that aggregate the collected articles, characterized by the predominance of a few sets of dimensions. We found that the impact on “financial markets” was widely studied, especially in relation to Asia. Next, we found a more diverse range of themes analyzed in Europe, from “government measures” to “macroeconomic variables.” We also discovered that America has not received the same degree of attention, and “institutions,” “Africa,” or “other pandemics” were studied less. We anticipate that future research will proliferate focusing on several themes, from environmental issues to the effectiveness of government measures.
Computational and Experimental Study of Film-Cooling Effectiveness With and Without Downstream Vortex Generators
Authors: Lee Chien-Shing , Shih Tom I-P. , Straub Douglas , Weber Justin M. , Robey Edward H. 
Published: September 2022

Abstract
Downstream vortex generators that involve a pair of rectangular plates arranged in an open V-shape placed just downstream of each film-cooling hole were shown to create flow and vortical structures that entrain lifted film-cooling flow back to the surface and increase its lateral spreading on the surface (GT2020–14317). In this study, computations and measurements were performed to examine the flow mechanisms this vortex generator induces to improve film-cooling effectiveness of a flat plate with the cooling flow emanating from one row of inclined holes. Parameters studied include blowing ratio (BR = 0.75 and 1.0) and temperature ratio (TR = 1.07 and 1.9). The computational study is based on steady Reynolds-averaged Navier–Stokes (RANS) closed by the shear-stress transport (SST) turbulence model with and without conjugate analysis. The experimental study was conducted by using a conjugate heat transfer test rig with a plenum, where cooling flow is introduced. Measurements made include velocity and temperature profiles upstream and downstream of the film-cooling holes as well as the temperature at several locations on the hot and cold sides of the film-cooled flat plate. The computational study was validated by comparing computed results with those from measurements at BR = 0.75 and 1.0 and TR = 1.9. Computational and experimental results are presented to show the effects of BR and TR on the flow structures and how those structures improve the effectiveness of film cooling with and without the downstream vortex generators and with and without conjugate heat transfer.
Critical Power, Work Capacity, and Recovery Characteristics of Team-Pursuit Cyclists
Authors: Pugh Charles F. , Beaven C. Martyn , Ferguson Richard A. , Driller Matthew W. , Palmer Craig D. , Paton Carl D. 
Published: September 2022

Purpose: Leading a 4-km team pursuit (TP) requires high-intensity efforts above critical power (CP) that deplete riders’ finite work capacity (W′), whereas riders following in the aerodynamic draft may experience some recovery due to reduced power demands. This study aimed to determine how rider ability and CP and W′ measures impact TP performance and the extent to which W′ can reconstitute during recovery positions in a TP race. Methods: Three TP teams, each consisting of 4 males, completed individual performance tests to determine their CP and W′. Teams were classified based on their performance level as international (INT), national (NAT), or regional (REG). Each team performed a TP on an indoor velodrome (INT: 3:49.9; NAT: 3:56.7; and REG: 4:05.4; min:s). Ergometer-based TP simulations with an open-ended interval to exhaustion were performed to measure individual ability to reconstitute W′ at 25 to 100 W below CP. Results: The INT team possessed higher CP (407 [4] W) than both NAT (381 [13] W) and REG (376 [15] W) (P < .05), whereas W′ was similar between teams (INT: 27.2 [2.8] kJ; NAT: 29.3 [2.4] kJ; and REG: 28.8 [1.6] kJ; P > .05). The INT team expended 104% (5%) of their initial W′ during the TP and possessed faster rates of recovery than NAT and REG at 25 and 50 W below CP (P < .05). Conclusions: The CP and rate of W′ reconstitution have a greater impact on TP performance than W′ magnitude and can differentiate TP performance level.
Systematic review of suicide prevention studies with data on youth and young adults living in low-income and middle-income countries
Authors: Doty Benjamin , Bass Judith , Ryan Taylor , Zhang Allen , Wilcox Holly 
Published: September 2022

ObjectivesThis review aimed to provide a summary of peer-reviewed, published literature on suicide preventive interventions with data on youth and young adults in low-income and middle-income countries (LMIC).DesignA systematic review was conducted using electronic databases of PubMed/MEDLINE, The Cochrane Library, Cumulative Index to Nursing and Allied Health Literature, PsycINFO, Education Resources Information Center and The Campbell Collaboration databases for English-language articles published between 1 January 1990 and 15 February 2022.Eligibility criteriaInterventions of interest could include behavioural, community, clinical/medical or policy studies, or any combination of these, so long as the studies had at least one outcome of interest and at least one control group or control period. Outcomes included suicide ideation, suicide attempt and suicide. Interventions must have been conducted in an LMIC. Studies with individuals ages 0–25 in the sample were included. Articles describing data on individuals over age 25 could be included if individuals ages 0–25 were part of the sample.ResultsA total of 44 eligible studies were identified, representing a broad range of universal, selective and indicated interventions. Most studies assessed interventions designed to address lethal means or mental health. Most studies were conducted in lower-middle-income or upper-middle-income countries, with the largest proportion in Asia. Assessment of outcomes across studies was heterogeneous and there were few large-scale investigations tailored specifically for youth.ConclusionsMost of the published, peer-reviewed suicide intervention research from LMIC is concentrated in a few countries. While geographical coverage to date has been limited, strategies and samples in included studies were diverse, representing populations in clinical, educational and community settings. While current findings hold promise, this review identified a need for large-scale studies designed specifically for youth.
Comparative efficacy of eight therapeutic methods in the treatment of left main coronary artery disease: a Bayesian network meta-analysis protocol
Authors: Hou Biao , Chen Manlin , Li Qin , Huang Weimin , Wang Liang 
Published: September 2022

IntroductionAs for coronary artery bypass grafting, although there are many direct comparative studies on different minimally invasive methods and traditional thoracotomy (off-pump/on-pump), there is still a lack of further ranking and summary of the efficacy of all surgical methods for left main coronary artery (LMCA) lesions. Combined with the current controversial views, this study aims to introduce a planned network meta-analysis (NMA) in detail, with a view to comparing the long-term efficacy and safety of multiple therapeutic methods in the treatment of patients with LMCA disease, and finally providing some reference bases for the best selection of clinical schemes.Method and analysisPubMed, Embase, Web of Science and The Cochrane Library databases will be collected from inception to June 2022 to compare the efficacy of different surgical methods in randomised controlled trials (RCTs) for LMCA disease. Main outcome endpoints: major adverse cardiovascular events, including mortality, myocardial infarction, stroke and revascularisation. Secondary outcome endpoints: (1) operation-related time, (2) the amount of blood transfusion, (3) complications including secondary thoracotomy, postoperative new atrial fibrillation, wound infection, (4) physiological score and psychological score, (5) time return to work and (6) total hospitalisation costs. The methodological quality of included RCTs will be assessed according to the Cochrane bias risk table. The Bayesian NMA will be conducted by STATA V.16.0.Ethics and disseminationThe essence of this study is to summarise and analyse the original data without the approval of the ethics committee. Our research does not involve ethical issues, and the results will be published in peer-review journals.PROSPERO registration numberCRD42021274712.
Prognostic value of FOXP3+ regulatory T cells in patients with diffuse large B-cell lymphoma: a systematic review and meta-analysis
Authors: Bai Yuping , He Tingting , Zhang Liyan , Liu Qianqian , Yang Jing , Zhao Ziru , Yang Kehu , Zhang Min 
Published: September 2022

ObjectivesWe aimed to comprehensively evaluate the relationship between forkhead box P3 (FOXP3+) regulatory T cell (Treg) expression and diffuse large B-cell lymphoma (DLBCL) prognosis and to explore the sources of heterogeneity of the results.DesignSystematic review and meta-analysis.Data sourcesWe searched the Cochrane Library, PubMed, Embase and Web of Science databases up to 5 December 2021.Eligibility criteriaWe included studies that analysed the prognostic significance of FOXP3+ Tregs in DLBCL. We included studies reported in Chinese or English that reported HRs and related 95% CIs for prognosis.Data extraction and synthesisWe extracted data from eligible studies. HRs and 95% CIs were used to assess the prognostic value.ResultsFourteen eligible studies were identified. FOXP3+ Treg expression was not associated with overall survival (OS) (HR=0.72, 95% CI 0.45 to 1.16) or progression-free survival (HR=0.86, 95% CI 0.54 to 1.38). The three approaches used to measure FOXP3+ Treg expression (pinteraction<0.001) may be the source of the heterogeneity of the results. Subgroup analysis found that a higher expression of FOXP3+ Tregs was associated with better OS in all populations and in Asians when FOXP3+ Treg expression was measured by the number of positive cells (HR=0.36 (95% CI 0.22 to 0.58) in the former, HR=0.33 (95% CI 0.20 to 0.55) in the latter) or the percentage of positive cells (HR=0.49 (95% CI 0.27 to 0.89) in the former, HR=0.38 (95% CI 0.21 to 0.70) in the latter). However, when measured by the score, inverse results were found (HR=1.56, 95% CI 1.01 to 2.42).ConclusionsApproaches to measuring FOXP3+ Treg expression might be the major source of heterogeneity in studies of the prognostic significance of FOXP3+ Tregs in DLBCL. FOXP3+ Treg expression might be used to predict the prognosis of patients with DLBCL when FOXP3+ Treg expression is calculated by the number or the percentage of positive cells, especially in Asian populations.
Geographical disparities and determinants of adherence to iron folate supplementation among pregnant women in Ethiopia: spatial and multilevel analysis of the Ethiopian Mini Demographic and Health Survey of 2019
Authors: Mulugeta Solomon Sisay 
Published: September 2022

ObjectiveThis study aimed to investigate geographic disparities and determinants of adherence to iron and folate supplementation among pregnant women in Ethiopia.MethodA secondary data analysis was performed using data from the Ethiopian Mini Demographic and Health Survey 2019. A total of 2235 pregnant women aged 15–49 years were included in the analysis. ArcGIS V.10.8 and SaTScan V.9.6 were used for spatial analysis. Multilevel logistic regression analysis was used to determinants.ResultOf the total number of participants, 80.3% of pregnant mothers took iron and folate supplements for less than the recommended days. Adherence to iron folate supplementation among pregnant women in Ethiopia was spatially clustered with Moran’s global I=0.15868. The SaTScan analysis identified the most likely significant clusters found in the eastern Tigray, northeast Amhara and northwest Afar regions. Multivariable multilevel analysis showed that mothers who were living apart from their partner (adjusted OR (AOR)=10.05, 95% CI 1.84 to 55.04), had antenatal care (ANC) visits at least four times (AOR=0.53, 95% CI 0.41 to 0.69), a higher education level (AOR=0.39, 95% CI 0.25 to 0.63), big distance from health facilities (AOR=1.7, 95% CI 1.51 to 1.97) were significant factors of adherence to iron-folate supplementation. Mothers living in the Amhara and Addis Ababa regions were 0.35 (AOR=0.35, 95% CI 0.19 to 0.621), and 0.29 (AOR=0.29, 95% CI 0.15 to 0.7) times lower iron-folate supplementation intake than mother’s in Tigray region.ConclusionIn this study, 8 out of 10 pregnant women did not take iron and folate supplements during the recommended period. As a result, health education activities were necessary to raise awareness among women and the community about the importance of iron folate supplementation during pregnancy, and public health programmes should increase iron folate supplementation through women’s education, ANC visits and mothers living in low-iron areas.
Validity of a catheter exit site clinical assessment scale for the early detection of exit site infections in patients on haemodialysis with a central venous catheter: protocol for a multicentre validation study in Spain (EXITA Study)
Authors: Cobo-Sánchez José Luis , Blanco-Mavillard Ian , Pelayo-Alonso Raquel , Mancebo-Salas Noelia , Fernandez-Fernandez Ismael , De Pedro-Gomez Joan Ernest 
Published: September 2022

IntroductionHaemodialysis patients with central venous catheter (HD-CVC) are at increased risk of exit site infections (ESIs) and catheter-related bloodstream infections, causing an increase of hospitalisation, morbidity and mortality rates. The main aim of the EXITA Study is to develop and validate an instrument for the early detection of HD-CVC ESIs.Methods and analysisEXITA is a multicentre prospective cohort study to validate the proposed instrument with a sample of 457 HD-CVCs: 92 in the ESI group and 365 in the non-ESI group. Sample size was calculated using Epidat V.4.2 software, with 95% and 90% expected sensitivity and specificity, respectively, an ESI incidence around 20% and 5%–10% precision range. During each haemodialysis session, the absence or presence of each item will be assessed by nurses. If any item is present, a microbiological study of pericatheter skin smears and/or exit site exudate will be carried out. HD-CVC ESI will be diagnosed when the pericatheter skin smears and/or exit site exudate culture are positive (≥15 CFU/mL by semiquantitative Maki’s technique or ≥1000 CFU/mL by Cleri’s technique). To validate the scale, a logistic regression analysis will be performed: the β coefficients of each of the signs/symptoms of the scale to be validated will be estimated. We will use logit function and calculate ESI probability=elogit ESI/1+elogit ESI.Ethics and disseminationThe study has been approved by the Research Ethics Committee with Medical Products of Cantabria (approval code 2019.146). We will obtain informed consent from all participants before data collection. We will publish the study results in a peer-reviewed scientific journal.