bluuebunny/crossref_metadata_2025_split huggingface dataset:
Continuous ex situ recovery of volatile monoterpenoids produced by genetically engineered Escherichia coli
Authors: Ayakar Sonal , Yadav Vikramaditya 
Published: June 2022

AbstractMonoterpenoids are a large group of natural, speciality chemicals. Their global demand is surging and the use of engineered microbial hosts such as Escherichia coli is a compelling strategy to sustainably and economically produce these molecules at scale. However, most monoterpenoids are highly volatile, which presents unique challenges for their recovery. The use of biphasic fermentations employing a dodecane overlay for product capture has emerged as a popular solution to this problem. However, since the subsequent separation of the products from dodecane is exceptionally difficult, biphasic fermentations are not practical. To this end, we report the development of an extraction methodology employing a fluidized bed of an aliphatic methacrylate resin for ex situ recovery of carene that is produced by a genetically engineered strain of E. coli. The resin is suspended in water within a 3D‐printed reservoir and volatilized carene is continuously captured by sparging the gaseous outflow from the condenser of the fermenter through the reservoir. We observed that the adsorption of carene onto the resin follows the Freundlich equation and that the extraction efficiency was chiefly influenced by the rate of agitation of the fermentation broth, the volume of resin enclosed within the reservoir, and the airflow rate into the fermenter. We subsequently used a fractional factorial experimental design to identify optimal levels of each parameter and thereafter improved the extraction efficiency six‐fold over that of a dodecane overlay. Our work establishes strong foundations for the use of solid phase‐transfer separations for the continuous recovery of volatile fermentation products.
Controlling energy aware clustering and multihop routing protocol for IoT assisted wireless sensor networks
Authors: Subramani Neelakandan , Perumal Santhosh Kumar , Kallimani Jagadish Shivappa , Ulaganathan Sakthi , Bhargava Sanjay , Meckanizi Sangeetha 
Published: June 2022

SummaryBecause of recent breakthroughs in information technology, the Internet of Things (IoT) is becoming increasingly popular in a variety of application areas. Wireless sensor networks (WSN) are a critical component of IoT systems, and they consist of a collection of affordable and compact sensors that are utilized for data collecting. WSNs are used in a variety of IoT applications, such as surveillance, detection, and tracking systems, to sense the surroundings and transmit the information to the user's device. Smart gadgets, on the other hand, are limited in terms of resources, such as electricity, bandwidth, memory, and computation. A fundamental issue in the IoT‐based WSN is to achieve energy efficiency while also extending the network's lifetime, which is one of the limits that must be overcome. As a result, energy‐efficient clustering and routing algorithms are frequently employed in the IoT system. As a result of this inspiration, the authors of this research describe an Energy Aware Clustering and Multihop Routing Protocol with mobile sink (EACMRP‐MS) technique for IoT supported WSN. The EACMRP‐MS technique's purpose is to efficiently reduce the energy consumption of IoT sensor nodes, consequently increasing the network efficiency of the IoT system. The suggested EACMRP‐MS technique initially relies on the Tunicate Swarm Algorithm (TSA) for cluster head (CH) selection and cluster assembly, as well as the TSA. Furthermore, the type‐II fuzzy logic (T2FL) technique is used for the optimal selection of multi‐hop routes, with multiple input parameters being used to achieve this. Finally, a mobile sink with route adjustment scheme is presented to further increase the energy efficiency of the IoT system. This scheme allows for the adjustment of routes based on the trajectory of the mobile sink, which further improves the energy efficiency of the system. Using a detailed experimental analysis and simulation findings, it was discovered that the EACMRP‐MS technique outperformed the most recent state of the art methods in terms of a variety of evaluation metrics, indicating that it is a promising alternative.
Singular value decomposition‐based behavior‐aware cloud service application programming interfaces recommendation for large‐scale software cloud directory platforms
Authors: Wang Lei , Zhang Yunqiu , Zheng Xubin , Yu Qi , Chen Shuhan , Ding Junyao 
Published: June 2022

SummaryWith the development of Internet technology and the cloud service industry, an increasing number of application programming interfaces (APIs) hosted in the cloud has been made publicly available. To facilitate cloud service APIs vendors and buyers, some large‐scale software cloud directory platforms have been established. Nevertheless, it is difficult for users to choose for renting from a massive number of cloud service APIs with similar functionalities in a software cloud directory platform. Recent efforts in building cloud service APIs recommender systems can help address this challenge. Relevant existing recommendation approaches are designed based on requirement election techniques to identify users' preferences to the quality of service (QoS) of the APIs. In particular, users' preferences are mainly obtained through their self‐description, in which users sometimes cannot accurately and completely express their preferences. In this article, we propose SVD‐APIR, a singular value decomposition (SVD)‐based behavior‐aware cloud service APIs recommendation approach for large‐scale software cloud directory platforms. In SVD‐APIR, users' historical behavior information is captured and APIs' association information is analyzed to identify the users' potential preferences to the APIs with specific QoS. A unified SVD model is utilized to prioritize the users preferred APIs. Experimental evaluation results conducted on WS‐Dream dataset demonstrate the effectiveness and efficiency of the proposed approach.
Genomewide Association Study of Simvastatin Pharmacokinetics
Authors: Mykkänen Anssi J. H. , Taskinen Suvi , Neuvonen Mikko , Paile‐Hyvärinen Maria , Tarkiainen E. Katriina , Lilius Tuomas , Tapaninen Tuija , Backman Janne T. , Tornio Aleksi , Niemi Mikko 
Published: June 2022

We investigated genetic determinants of single‐dose simvastatin pharmacokinetics in a prospective study of 170 subjects and a retrospective cohort of 59 healthy volunteers. In a microarray‐based genomewide association study with the prospective data, the SLCO1B1 c.521T>C (p.Val174Ala, rs4149056) single nucleotide variation showed the strongest, genomewide significant association with the area under the plasma simvastatin acid concentration‐time curve (AUC; P = 6.0 × 10−10). Meta‐analysis with the retrospective cohort strengthened the association (P = 1.6 × 10−17). In a stepwise linear regression candidate gene analysis among all 229 participants, SLCO1B1 c.521T>C (P = 1.9 × 10−13) and CYP3A4 c.664T>C (p.Ser222Pro, rs55785340, CYP3A4*2, P = 0.023) were associated with increased simvastatin acid AUC. Moreover, the SLCO1B1 c.463C>A (p.Pro155Thr, rs11045819, P = 7.2 × 10−6) and c.1929A>C (p.Leu643Phe, rs34671512, P = 5.3 × 10−4) variants associated with decreased simvastatin acid AUC. Based on these results and the literature, we classified the volunteers into genotype‐predicted OATP1B1 and CYP3A4 phenotype groups. Compared with the normal OATP1B1 function group, simvastatin acid AUC was 273% larger in the poor (90% confidence interval (CI), 137%, 488%; P = 3.1 × 10−6), 40% larger in the decreased (90% CI, 8%, 83%; P = 0.036), and 67% smaller in the highly increased function group (90% CI, 46%, 80%; P = 2.4 × 10−4). Intermediate CYP3A4 metabolizers (i.e., heterozygous carriers of either CYP3A4*2 or CYP3A4*22 (rs35599367)), had 87% (90% CI, 39%, 152%, P = 6.4 × 10−4) larger simvastatin acid AUC than normal metabolizers. These data suggest that in addition to no function SLCO1B1 variants, increased function SLCO1B1 variants and reduced function CYP3A4 variants may affect the pharmacokinetics, efficacy, and safety of simvastatin. Care is warranted if simvastatin is prescribed to patients carrying decreased function SLCO1B1 or CYP3A4 alleles.
A low‐power amplitude shift keying modulator based on adaptive body‐biased injection‐locked current‐reuse voltage controlled oscillator for high data rate radio frequency identification applications
Authors: Yasir Umair , Li Xiuping , Cao Cheng , Tao Tan 
Published: June 2022

AbstractA low‐power consuming amplitude‐shift keying (ASK) modulator designed in 130 nm CMOS technology and operating at 10.5 GHz frequency is presented in this work. The modulator is based on current‐reuse voltage‐controlled oscillator (VCO) which uses the effects of adaptive body‐biasing and injection locking (IL) to achieve low‐phase noise and low‐power operation. The VCO has a phase noise of −106 dBc/Hz at 1 MHz offset. The ASK modulator consumes 790 μW with a 1.1 V voltage supply and draws an average current of 719 μA. For applying the bulk‐biasing signal, a deep n‐well NMOS is used in the PMOS‐NMOS oscillator pair which isolates the substrate and avoids the current path through it. The post‐layout simulation results of the 0.5 × 0.5 mm2 modulator IC verify its low‐power, low‐phase noise operation. It is intended for use in self‐powered active high data rate radio‐frequency identification (RFID) tags.
TGF‐β1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation
Authors: Baik Jung Eun , Park Hyeung Ju , Kataru Raghu P. , Savetsky Ira L. , Ly Catherine L. , Shin Jinyeon , Encarnacion Elizabeth M. , Cavali Michele R. , Klang Mark G. , Riedel Elyn , Coriddi Michelle , Dayan Joseph H. , Mehrara Babak J. 
Published: June 2022

AbstractBackgroundSecondary lymphedema is a common complication of cancer treatment, and previous studies have shown that the expression of transforming growth factor‐beta 1 (TGF‐β1), a pro‐fibrotic and anti‐lymphangiogenic growth factor, is increased in this disease. Inhibition of TGF‐β1 decreases the severity of the disease in mouse models; however, the mechanisms that regulate this improvement remain unknown.MethodsExpression of TGF‐β1 and extracellular matrix molecules (ECM) was assessed in biopsy specimens from patients with unilateral breast cancer‐related lymphedema (BCRL). The effects of TGF‐β1 inhibition using neutralizing antibodies or a topical formulation of pirfenidone (PFD) were analyzed in mouse models of lymphedema. We also assessed the direct effects of TGF‐β1 on lymphatic endothelial cells (LECs) using transgenic mice that expressed a dominant‐negative TGF‐β receptor selectively on LECs (LECDN‐RII).ResultsThe expression of TGF‐β1 and ECM molecules is significantly increased in BCRL skin biopsies. Inhibition of TGF‐β1 in mouse models of lymphedema using neutralizing antibodies or with topical PFD decreased ECM deposition, increased the formation of collateral lymphatics, and inhibited infiltration of T cells. In vitro studies showed that TGF‐β1 in lymphedematous tissues increases fibroblast, lymphatic endothelial cell (LEC), and lymphatic smooth muscle cell stiffness. Knockdown of TGF‐β1 responsiveness in LECDN‐RII resulted in increased lymphangiogenesis and collateral lymphatic formation; however, ECM deposition and fibrosis persisted, and the severity of lymphedema was indistinguishable from controls.ConclusionsOur results show that TGF‐β1 is an essential regulator of ECM deposition in secondary lymphedema and that inhibition of this response is a promising means of treating lymphedema.
Effects of an attachment‐based intervention on autonomic regulation among opioid‐exposed infants
Authors: Tabachnick Alexandra R. , Eiden Rina Das , Labella Madelyn H. , Dozier Mary 
Published: June 2022

AbstractLittle is known about whether postnatal intervention enhances autonomic regulation among infants at risk for dysregulation due to prenatal opioid exposure. The present study evaluated the effects of modified Attachment Behavioral Catch‐up (mABC) on autonomic regulation for opioid‐exposed infants in a pilot randomized clinical trial. We hypothesized that, compared to a control intervention (modified Developmental Education for Families [mDEF]), mABC would be associated with higher resting respiratory sinus arrhythmia (RSA) and pre‐ejection period (PEP) as well as greater reactivity to and recovery from a social stressor (Still‐Face Paradigm). Pregnant or peripartum women receiving opioid agonist therapy (61 mothers of 64 infants; final N = 36 infants) were randomly assigned to mABC or mDEF, 12‐session home visiting programs beginning in the third trimester; mABC targets sensitive parenting, and mDEF targets cognitive and motor development. mABC was associated with significantly greater RSA reactivity and marginally greater PEP reactivity. In models accommodating missing data, mABC was additionally associated with significantly greater RSA recovery. In sensitivity analyses removing siblings, mABC predicted significantly enhanced PEP reactivity. Overall, in these preliminary analyses, mABC was associated with healthier autonomic regulation during a social stressor than mDEF. Thus, mABC may be a promising strategy to promote autonomic regulation among opioid‐exposed infants through parenting intervention.
The role of Hippo‐YAP/TAZ signaling in brain development
Authors: Terry Bethany K. , Kim Seonhee 
Published: June 2022

AbstractIn order for our complex nervous system to develop normally, both precise spatial and temporal regulation of a number of different signaling pathways is critical. During both early embryogenesis and in organ development, one pathway that has been repeatedly implicated is the Hippo‐YAP/TAZ signaling pathway. The paralogs YAP and TAZ are transcriptional co‐activators that play an important role in cell proliferation, cell differentiation, and organ growth. Regulation of these proteins by the Hippo kinase cascade is therefore important for normal development. In this article, we review the growing field of research surrounding the role of Hippo‐YAP/TAZ signaling in normal and atypical brain development. Starting from the development of the neural tube to the development and refinement of the cerebral cortex, cerebellum, and ventricular system, we address the typical role of these transcriptional co‐activators, the functional consequences that manipulation of YAP/TAZ and their upstream regulators have on brain development, and where further research may be of benefit.
Metabarcoding analysis of different portions of the digestive tract of scorpions (Scorpiones, Arachnida) following a controlled diet regime shows long prey DNA half‐life
Authors: Simone Yuri , Chaves Cátia , van der Meijden Arie , Egeter Bastian 
Published: June 2022

AbstractMolecular analysis of gut content is one of the widest used methods to investigate diet in arthropods. Stomach content analysis in some arthropods is particularly difficult, e.g., in arachnids, because they have external digestion and a low foraging frequency. Scorpions have a particularly low feeding frequency, and their diet information is scarce. In this work we explore a DNA metabarcoding approach to detect prey DNA in Vietnamese forest scorpions (Heterometrus laoticus) under a controlled diet regime. A different type of prey (crickets, mealworms, and cockroaches) was offered once every 3 weeks for a total of 9 weeks. To assess the most suitable part of the digestive system and extraction method to use for molecular diet analysis, we separately analyzed three different portions of the digestive tract of scorpions (mid‐gut, hepatopancreas, and hindgut) using two different extraction methods (salt‐out method and a customized beads‐based protocol). We calculated the detectability half‐life of the prey DNA for each digestive tract section. We detected all three targeted prey items, showing that in scorpions multiple predation events can be distinguished in the same specimen within its last 9 weeks of foraging activity. The hepatopancreas was the portion of the digestive tract that provided the best prey detection and the longest DNA detectability half‐life (51 days), followed by the mid‐gut (22 days) and the hindgut (16 days). We found no significant difference between the extraction methods used. However, the salt‐out method was less effective in some of the PCRs and is therefore not recommended for molecular diet analysis.
The impact of different intensities and domains of physical activity on analgesic use and activity limitation in people with low back pain: A prospective cohort study with a one‐year followup
Authors: Patterson Thomas G. , Beckenkamp Paula R. , Ferreira Manuela , Bauman Adrian , Carvalho‐e‐Silva Ana Paula , Ferreira Lucas Calais , Ferreira Paulo H. 
Published: June 2022

AbstractBackgroundAnalgesics are the most common form of managing low back pain (LBP). No previous study has examined which domains and intensities of physical activity are most beneficial in reducing the frequency of analgesic use for LBP and its related activity limitation.MethodsThis cohort study forms part of the AUstralian Twin low BACK pain study, investigating the impact of physical activity on LBP. Information on demographics, LBP and health‐related factors, including physical activity, were collected at baseline. Data on the total counts of analgesic use and activity limitation for LBP were collected weekly for one‐year. Negative binomial regression models were conducted separately for each type of physical activity. Results were presented as incidence rate ratios (IRRs) and 95% confidence intervals (CIs).ResultsFrom an initial sample of 366 participants, 86 participants reported counts of analgesic use and 140 recorded counts of activity limitation across the follow‐up period. The negative binomial regression models for analgesic use counts indicated that engagement in moderate‐vigorous physical activity was protective for use of analgesics (IRR 0.97, 95% CI 0.96–0.99), while physical workload was associated with greater use (IRR 1.02, 95% CI 1.01–1.05). No other significant relationships were observed for the other measures of physical activity. For activity limitation counts, engagement in leisure activity was associated with less counts of activity limitation (IRR 0.94, 95% CI 0.81–0.99), while greater amounts of sedentary time was associated with higher counts (IRR 1.04, 95% CI 1.01–1.09). No other significant relationships were observed for the other measures of physical activity.ConclusionsOur findings highlight the potential importance of supporting engagement in moderate–vigorous and leisure physical activity as well as minimizing sedentary time and physical workload to reduce the risk of activity limitation and the need for analgesic use in people with LBP.SignificanceWe examined which domains and intensities of physical activity are most beneficial in reducing the frequency of analgesic use for low back pain and its related activity limitation. Engaging in moderate–vigorous and leisure physical activity as well as minimizing sedentary time and physical workload has the potential to reduce the risk of activity limitation and the need for analgesic use in people with low back pain.
Graphene decorated LiMn2O4 electrode material for hybrid type energy storage devices
Authors: Mandal Debabrata , Bharti Lalit , Biswas Sudipta , Chandra Amreesh 
Published: June 2022

AbstractLithium‐ion capacitors (LICs) are becoming useful owing to their high energy and power densities. LICs show enhance capacitance than electric double‐layer capacitance (EDLC) and pseudocapacitors, due to their battery electrode material, which can operate over a higher voltage window. LiMn2O4 is fast emerging electrode material for energy storage devices. The major limitation associated with it is the low conductivity. Therefore, to simultaneously improve the electrical conductivity as well as the electrochemical performance, a novel strategy of decoration LiMn2O4 with graphene is being proposed. Graphene will improve electrical conductivity while contributing to EDLC and pseudocapacitance values. A cost‐effective synthesis protocol is utilized to obtain conical pyramid‐type particles. These particles were thoroughly characterized using a large number of experimental techniques. Cyclic voltammetry and charge‐discharge measurements were used to evaluate the electrochemical characteristics, in 0.0 to 1.2 V stable window, using 1M LiOH electrolyte. Graphene decorated LiMn2O4 electrode show improved cyclic stability and higher specific capacitances 250 F/g in comparison to LiMn2O4 electrode. The cycling stability of the electrode was above 90% up to 3000 cycles at a higher current density.
Delivering health programs for Aboriginal and Torres Strait Islander children: Carer and staff views on what's important
Authors: Chando Shingisai , Dickson Michelle , Howell Martin , Tong Allison , Craig Jonathan C. , Slater Kym , Smith Natalie , Nixon Janice , Eades Sandra J. , Howard Kirsten 
Published: June 2022

AbstractBackgroundThe lack of evidence on the priorities of carers and their Aboriginal and Torres Strait Islander children undermines decisions to improve participant experiences and engagement.AimsThis study describes carer and staff perspectives on the aspects of health services delivery that are important to carers and children.MethodsNineteen carers of Aboriginal and Torres Strait Islander children and 17 staff who work at child health programs across two urban Aboriginal Community Controlled Health Services (ACCHSs) and affiliate organisations in New South Wales, Australia participated in semi‐structured interviews. We used thematic analysis to analyse the data.ResultsWe identified five themes: valuing relational communication (building trust by keeping relationships at the centre, empowered to optimise child's development, feeling heard and known); confidence in provider's clinical and interpersonal skills (certain that the health issue will be resolved, engaging with the child to allay fears, facilitating timely health care); finding comfort and security in community embedded services (safety and acceptance in the familiar, strengthening child's connection to culture); support to access and navigate health services (accessible information appropriately presented, easy and flexible scheduling, easing the shame of financial hardship); sustaining service use (fulfilling expectations for service standards, demonstrating commitment through ongoing programs, clarity of benefits).ConclusionsCarers and staff reported that approaches to communication, the content of that communication, how access is facilitated and the service environment managed influences their decisions to interact with health services. With these data decision‐makers can better focus resources to improve experiences with their services.