bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collateral cleavage of 28s rRNA by RfxCas13d causes death of mice
Authors: Li Yunfei , Xu Junjie , Guo Xuefei , Li Zhiwei , Cao Lili , Liu Shengde , Guo Ying , Wang Guodong , Luo Yujie , Zhang Zeming , Wei Xuemei , Zhao Yingchi , Liu Tongtong , Wang Xiao , Xia Huawei , Kuang Ming , Guo Qirui , Li Junhong , Chen Luoying , Wang Yibing , Li Qi , Wang Fengchao , Liu Qinghua , You Fuping 
Published: January 2022

SummaryThe CRISPR-Cas13 system is an RNA-guided RNA-targeting system, and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Here, we found that knocking down gene expression using RfxCas13d in the adult brain neurons caused death of mice, which was not resulted from the loss of target gene function or off-target effects. Mechanistically, we showed that RfxCas13d exhibited collateral activity in mammalian cells, which is positively correlated with the abundance of target RNA. The collateral activity of RfxCas13d could cleave 28s rRNA into two fragments, leading to translation attenuation and activation of the ZAKα-JNK/p38-immediate early gene (IEG) pathway. These results provide new mechanistic insights into the collateral activity of RfxCas13d and warn that the biosafety of CRISPR-Cas13 system needs further evaluation before applying it to clinical treatments.
The Idiopathic Pulmonary Fibrosis-associated single nucleotide polymorphism rs35705950 is transcribed in a MUC5B Promoter Associated Long Non-Coding RNA (AC061979.1)
Authors: Neatu R. , Thompson D.J. , Enekwa I. , Schwalbe E.C. , Fois G. , Frick M. , Braubach P. , Moschos S.A. 
Published: January 2022

AbstractLncRNAs are involved in regulatory processes in the human genome, including gene expression. The rs35705950 SNP, previously associated with IPF, overlaps the recently annotated lncRNA AC061979.1, a 1,712 nucleotide transcript located within the MUC5B promoter at chromosome 11p15.5. To document the expression pattern of the transcript, we processed 3.9 TBases of publicly available RNA-SEQ data across 27 independent studies involving lung airway epithelial cells. Epithelial lung cells showed expression of this putative pancRNA. The findings were independently validated in cell lines and primary cells. The rs35705950 is found within a conserved region (from fish to primates) within the expressed sequence indicating functional importance. These results implicate the rs35705950-containing AC061979.1 pancRNA as a novel component of the MUC5B expression control minicircuitry.
Crystal structure of bismuth-containing NdFe3(BO3)4 in the temperature range 20–500 K
Authors: Smirnova Ekaterina S. , Alekseeva Olga A. , Dudka Alexander P. , Verin Igor A. , Artemov Vladimir V. , Lyubutina Marianna V. , Gudim Irina A. , Frolov Kirill V. , Lyubutin Igor S. 
Published: January 2022

Neodymium iron borate NdFe3(BO3)4 is an intensively studied multiferroic with high electric polarization values controlled by a magnetic field. It is characterized by a large quadratic magnetoelectric effect, rigidity in the base plane and a rather strong piezoelectric effect. In this work, the atomic structure of (Nd0.91Bi0.09)Fe3(BO3)4 was studied by single-crystal X-ray diffraction in the temperature range 20–500 K (space group R32, Z = 3). The Bi atoms found in the composition partially substitute the Nd atoms in the 3a position; they entered the structure due to the growth conditions in the presence of Bi2Mo3O12. It was shown that in the temperature range 20–500 K there is no structural phase transition R32→P3121, which occurs in rare-earth iron borates (RE = Eu–Er, Y) with an effective rare-earth cation radius smaller than that of Nd. The temperature dependence of the unit-cell c parameter reveals a slight increase on cooling below 90 K, which is similar to the results obtained previously for iron borates of Gd, Y and Ho. The atomic distances (Nd,Bi)—O, (Nd,Bi)—B, (Nd,Bi)—Fe, Fe—O, Fe—B and Fe—Fe in the iron chains and between chains decrease steadily with decreasing temperature from 500 to 90 K, whereas the B1(3b)—O distance does not change and the average B2(9e)—O distance increases slightly. There is a uniform decrease in the atomic displacement parameters with decreasing temperature, with a more pronounced decrease for the Nd(3a) and O2(9e) atoms. The O2(9e) atoms are characterized by the maximum atomic displacement parameters and the most elongated atomic displacement ellipsoids. The characteristic Debye and Einstein temperatures, and the static component in the atomic displacements were determined for cations using multi-temperature diffraction data. It was shown that the Nd cations have the weakest bonds with the surrounding atoms and the B cations have the strongest.
Development of special needs classroom prototypes to respond to the sensory needs of students with exceptionalities
Authors: Patel Tina , Dorff Juliann , Baker Allison 
Published: January 2022

PurposeThe observation by the authors, based on their extensive experience working in K-8 public schools in the region showed the special needs classrooms catering to children with exceptionalities such as Autism Spectrum Disorder (ASD), Intellectual Disability (ID) and Emotional Disturbance (ED) are typically makeshift arrangements with no consideration given to students' unseen sensory needs. A thorough literature review indicates that there are no holistic design guidelines in place to meet the sensory needs of students with ASD, ID and ED. This study seeks to address this gap by providing considerations to meet the sensory needs impacting these students' focus, behavior and classroom engagement with course content and peers.Design/methodology/approachSensory design guidelines were established utilizing a qualitative method, providing a foundation for the development of classroom prototypes that address the sensory needs of students with ASD, ID and ED.FindingsThe new guidelines, which correlated interior design strategies with the sensory needs of children with ASD, ID and ED, and the resulting prototypes provide a basis for the further development of design standards and takes designers closer to creating more conducive and inclusive environments.Practical implicationsThis study reinforced the belief that these recommendations should be considered in the school-wide design. Many students can be included with their typical peers for all or part of their school day if space has been designed to accommodate their differences.Originality/valueThis study bridges the gap while documenting the correlation between design factors and sensory needs of students with exceptionalities, in this case, ASD, ID and ED.
Linking perceived institutional force and environmental strategy: the moderating role of institutional incompleteness
Authors: Feng Taiwen , Liu Shasha , Zhang Qiansong 
Published: January 2022

PurposeThis study aims to examine the impact of perceived institutional force on environmental strategy, and the moderating role of institutional incompleteness.Design/methodology/approachThis study tests hypotheses employing hierarchical regression model based on a survey of 317 Chinese manufacturers.FindingsThe results reveal that perceived business and social force have positive impacts on symbolic environmental strategy. Perceived social force has a positive impact on substantive environmental strategy and a negative impact on greenwashing. Further analysis suggests that perceived social force has a stronger effect on substantive environmental strategy than perceived business force. Moreover, institutional incompleteness strengthens the impact of perceived business force on substantive environmental strategy, and the impact of perceived social force on symbolic and substantive environmental strategy.Originality/valueThis study establishes a framework integrating distinct types of perceived institutional force and environmental strategy and provides a new perspective on measuring greenwashing to extend environmental strategy literature.
Dietary intervention of prebiotics and vitamins on gut health of children
Authors: Kaushal Deepti , Kalsi Gurpreet 
Published: January 2022

Purpose
Gut health has multidimensional impact on childhood growth and development. Diet being the major modulator of gut health, this study aims to hypothesize if supplementation of dietary prebiotics and vitamins can impact gut environment of healthy children.

Design/methodology/approach
A double-blind placebo-controlled trial was conducted with parallel allocation of children 8–13 years of age to placebo or test group. Test group was supplemented with 1.5 g of formulation comprising prebiotics and vitamins, and to placebo, maltodextrin (1.5 g) was supplemented. After four weeks, the effect on markers of gut health (i.e. beneficial microbes, and gut integrity markers such as secretory Immunoglobulin A [sIgA] and calprotectin concentration) was evaluated in feces of children.

Findings
Significant increase of 17.1% in fecal bifidobacteria counts (p = 0.04) was observed in the test group compared to placebo after four weeks. Also, fecal calprotectin concentration decreased (20%) significantly (p = 0.01) in test group compared to placebo. In addition, the test group showed significant improvement in fecal sIgA and lactobacilli compared to baseline concentration (p = 0.01 and 0.001, respectively).

Research limitations/implications
This study demonstrates that prebiotic (and vitamin) intervention may influence health outcomes in children by positively modulating markers of gut health.

Originality/value
There are limited studies demonstrating the efficacy of prebiotic and vitamins on gut health of school age children.
Authoritarian and benevolent leadership: the role of follower homophily, power distance orientation and employability
Authors: Koveshnikov Alexei , Ehrnrooth Mats , Wechtler Heidi 
Published: January 2022

PurposeDrawing on follower-centric leadership theory, the study examines the role of perceived homophily between the leader and the follower, follower's individual-level power distance orientation (PDO) and follower's perceived employability in moderating the effects of authoritarian and benevolent paternalistic leadership (BPL) on followers' turnover intentions.Design/methodology/approachThe study analyzes a sample of 403 white-collar Russian employees.FindingsWhereas both leadership styles generally decrease followers' turnover intentions, they operate differently. Authoritarian leadership (AL) is more effective among followers with higher follower-leader homophily and PDO, whereas BPL is effective only among followers with low perceived homophily and PDO, and more effective among followers with higher perceived employability.Originality/valueThe study extends research on non-participative styles of leadership, their effects and boundary conditions.
What sort of collective bargaining is emerging in Nigeria?
Authors: Opute John Ebinum , Mahmoud Ali B. 
Published: January 2022

PurposeNigeria is experiencing an expanding variety of what is termed collective bargaining, which is being propelled by socio-economic challenges and the emerging political dispensation that had long eluded the country, albeit the numerous contours needing some pragmatic approaches from the state, employers of labour and the trade unions at the local and national levels. Therefore, this study represents an attempt to illustrate the rising collective bargaining pattern in Nigeria.Design/methodology/approach This study drew on employee and employer sectoral associations examples together with labour union structures of the state to assess what underlined collective bargaining developments from the broad context of collective bargaining and the industrial relations implications. Content analysis was employed to analyse the secondary data (found in relevant company handbooks, policies, collective agreements, etc.) and primary data obtained through unstructured interviews.FindingsA form of collective bargaining is emerging where the trade unions are embracing symbiotic agreements at plant levels to improve conditions of employment and thus weakening the hold of the national union from collective bargaining – a move that may challenge the conceptual framework of collective bargaining as conceived by many states in developing economies.Originality/valueThis is an investigative paper, carefully trailing the framework of collective bargaining from direct contacts with all stakeholders in the labour corridors of Nigeria – such as the Nigeria Labour Congress, Nigeria Employers’ Consultative Association, Chemical and Non-Metallic Products Employer's Federation, Metal Products Workers Union of Nigeria and Personnel Practitioners, cutting across all the segments of the political and economic development of the country.
Harmonizing taxon names in biodiversity data: A review of tools, databases and best practices
Authors: Grenié Matthias , Berti Emilio , Carvajal‐Quintero Juan , Dädlow Gala Mona Louise , Sagouis Alban , Winter Marten 
Published: January 2022

Abstract

The process of standardizing taxon names, taxonomic name harmonization, is necessary to properly merge data indexed by taxon names. The large variety of taxonomic databases and related tools are often not well described. It is often unclear which databases are actively maintained or what is the original source of taxonomic information. In addition, software to access these databases is developed following non‐compatible standards, which creates additional challenges for users. As a result, taxonomic harmonization has become a major obstacle in ecological studies that seek to combine multiple datasets.

Here, we review and categorize a set of major taxonomic databases publicly available as well as a large collection of R packages to access them and to harmonize lists of taxon names. We categorized available taxonomic databases according to their taxonomic breadth (e.g. taxon specific vs. multi‐taxa) and spatial scope (e.g. regional vs. global), highlighting strengths and caveats of each type of database. We divided R packages according to their function, (e.g. syntax standardization tools, access to online databases, etc.) and highlighted overlaps among them. We present our findings (e.g. network of linkages, data and tool characteristics) in a ready‐to‐use Shiny web application (available at: https://mgrenie.shinyapps.io/taxharmonizexplorer/).

We also provide general guidelines and best practice principles for taxonomic name harmonization. As an illustrative example, we harmonized taxon names of one of the largest databases of community time series currently available. We showed how different workflows can be used for different goals, highlighting their strengths and weaknesses and providing practical solutions to avoid common pitfalls.

To our knowledge, our opinionated review represents the most exhaustive evaluation of links among and of taxonomic databases and related R tools. Finally, based on our new insights in the field, we make recommendations for users, database managers and package developers alike.
Editorial: an accurate, reliable and responsive ultrasound activity score for Crohn’s disease? Sounds good to US
Authors: Goodsall T.M. , Bryant R.V. 
Published: January 2022

LINKED CONTENTThis article is linked to Allocca et al papers. To view these articles, visit https://doi.org/10.1111/apt.16700 and https://doi.org/10.1111/apt.16754
Letter: the hidden reasons of long‐term risk of upper gastrointestinal bleeding after Helicobacter pylori eradication
Authors: Yang Hang , Hu Bing 
Published: January 2022

LINKED CONTENTThis article is linked to Jiang et al papers. To view these articles, visit https://doi.org/10.1111/apt.16604 and https://doi.org/10.1111/apt.16746
Editorial: testing for Epstein Barr virus and treating autoimmune hepatitis—still a long way to go! Authors' reply
Authors: Nayagam Jeremy S. , Heneghan Michael A. , Samyn Marianne , Joshi Deepak 
Published: January 2022

LINKED CONTENTThis article is linked to Nayagam et al papers. To view these articles, visit https://doi.org/10.1111/apt.16708 and https://doi.org/10.1111/apt.16751
Youth crime and depictions of youth crime in Canada: Are news depictions purely moral panic?
Authors: Silcox Jennifer 
Published: January 2022

AbstractScholars have highlighted the ways media often exaggerate and sensationalize crime in the western world. Nevertheless, it remains unclear whether the media's portrayal of youth crime offers an objective assessment of real‐world social problems, a sensationalized reflection of real‐world trends, or whether it signals a moral panic surrounding youth crime and violence. The purpose of the paper is to examine newspaper accounts of youth crime in comparison to official youth crime statistics and to determine whether, and to what extent, they align. If they do not align, can the existence of a moral panic be said to influence news portrayals of Canadian youth in conflict with the law?
Normal‐tension glaucoma: Current concepts and approaches‐A review
Authors: Leung Dexter Y. L. , Tham Clement C. 
Published: January 2022

AbstractNormal tension glaucoma (NTG) has remained a challenging disease. We review, from an epidemiological perspective, why we should redefine normality, act earlier at lower pre‐treatment intraocular pressure (IOP) level, and the role of ocular perfusion pressures, noting that perfusion is affected by defective vascular bed autoregulation and endothelial dysfunction. The correlation of silent cerebral infarcts (SCI) and NTG may indicate that NTG belongs to a wider spectrum of small vessel diseases (SVD), with its main pathology being also on vascular endothelium. Epidemiological studies also suggested that vascular geometry, such as fractal dimension, may affect perfusion efficiency, occurrence of SCI, SVD and glaucoma. Artificial intelligence with deep learning, may help predicting NTG progression from vascular geometry. Finally, we review latest evidence on the role of minimally‐invasive glaucoma surgery, lasers, and newer drugs. We conclude that IOP is not the only modifiable risk factors as, many vascular risk factors are readily modifiable.
Additional preconditioning program for bariatric surgery: Any benefits? A large cohort study
Authors: van der Ven Roos G. F. M. , de Vos Rien , Lodewijks Yentl , van Hout Gerbrand C. M. , Lichthart Sacha , Nienhuijs Simon W. 
Published: January 2022

SummaryPostbariatric outcomes may improve by providing an additional preconditioning program (APP) in targeted patients. However, APPs are a demand for health resources while only little and inconsistent evidence consists to support their effectiveness. This cohort study aims to evaluate the effectiveness of APP, by comparing outcomes of patients with and without such APP. We carried out a retrospective single‐centre cohort study in a before‐after design. Patients signing up for primary gastric bypass or sleeve gastrectomy and eligible for surgery were included if screened as vulnerable patients. Vulnerable patients screened between September 2017 and March 2018 followed an APP and formed the APP‐group. Due to a policy change, APPs were no longer performed since September 2018. Vulnerable patients screened between September 2018 and March 2019 thus did not receive an APP (comparator‐group). Multidisciplinary follow‐up remained unchanged. Endpoints included percentage total weight loss (%TWL), bodyweight, evolution of comorbidities, protein intake, and number of no‐shows. The APP‐group comprised 231 patients and the comparator‐group 153. %TWL differed statistically significantly at 7 (Δ1.5%, p = .01) and 12 months postoperative (Δ2.8%, p < .01) in favour of the comparator‐group, as did bodyweight 12 months postoperative (Δ1.8 kg, p < .01). Statistically significant differences were also found in the evolution of comorbidities, protein intake, and the number of no‐shows, most in favour for the comparator‐group. APP proofed not to be superior to Non‐App. It is debatable whether statically significant differences are clinically relevant given their small magnitude. A care pathway without an APP seems at least as effective as a care pathway without.
Availability of PEth testing is associated with reduced eligibility for liver transplant among patients with alcohol‐related liver disease
Authors: Selim Ranya , Zhou Yueren , Rupp Loralee B. , Trudeau Sheri , Naffouj Sandra , Shamaa Omar , Ahmed Abdelwahab , Jafri Syed‐Mohammed , Gordon Stuart C. , Segal Antu , Gonzalez Humberto C. 
Published: January 2022

AbstractBackgroundSerum phosphatidylethanol (PEth) is a highly sensitive test to detect alcohol use. We evaluated whether the availability of PEth testing impacted rates of liver transplant evaluation terminations and delistings.MethodsMedical record data were collected for patients who initiated transplant evaluation due to alcohol‐related liver disease in the pre‐PEth (2017) or PEth (2019) eras. Inverse probability weighting (IPW) was used to balance baseline patient characteristics. Outcomes included termination of evaluation or delisting due to alcohol use; patients were censored at receipt of transplant; death was considered a competing risk. The Fine‐Gray method was performed to determine whether PEth testing affected risk of evaluation termination/ delisting due to alcohol use.ResultsThree hundred and seventy‐five patients with alcohol‐related indications for transplant (157 in 2017; 210 in 2019) were included. The final IPW‐adjusted model for the composite outcome of terminations/delisting due to alcohol use retained two significant variables (P < .05): PEth era and BMI category. Patients evaluated during the PEth era were almost three times more likely to experience an alcohol‐related termination/delisting than those in the pre‐PEth era (sHR = 2.86; 95%CI 1.67–4.97)ConclusionWe found that availability of PEth testing at our institution was associated with a higher rate of exclusion of patients from eligibility for liver transplant. Use of PEth testing has significant potential to inform decisions regarding transplant candidacy for patients with alcohol‐related liver disease.