bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Novel Quantitative Measurement of Iris Angiography in Diabetic Retinopathy
Authors: Zhang Longli , Chai Yue , Zhang Luyuan , Hei Kaiwen , Li Xiaorong 
Published: June 2022

Abstract
Background: The purpose of this study was to explore the application of a novel quantitative measurement of iris angiography (IA) in diabetic retinopathy (DR).Methods: This was a single-center, cross-sectional prospective study. Totally 168 consecutive patients with diabetic mellitus (DM) were involved in this study from May 2016 to December 2020, 168 subjects were divided into 3 groups, 32 non-retinopathy patients,96 non-proliferative diabetic retinopathy patients (NPDR), and 40 proliferative diabetic retinopathy (PDR) patients. All patients underwent ophthalmologic examination, such as best corrected visual acuity, intraocular pressure, slit-lamp microscopy, gonioscopy, ultra-wide-field fundus photography, fluorescein angiography of the iris (IFA), and indocyanine green angiography of the iris (IIGA). The starting of iris vascular leakage time (LT) was recorded, and the range of pupil fluorescein leakage was measured by a novel measurement. Results: LT of iris in non-retinopathy, non-proliferative diabetic retinopathy (NPDR), and proliferative diabetic retinopathy (PDR) groups were 33.14 ± 3.03s, 32.45 ± 5.17s, and 25.67 ± 5.03s, respectively. The LT in the PDR group was significantly faster than that in the NPDR and non-retinopathy groups (P=0.000). The iris pupil leakage range (LR) of the non-retinopathy, NPDR, and PDR groups were 21.21 ± 30.06, 62.48 ± 42.17, and 141.31 ± 73.61°, and LR in PDR groups were largest among all groups (P=0.000). Neovascularization of the iris (NVI) was detected in 8 eyes (26.7%) in the PDR group. No NVI was found in non-retinopathy and NPDR groups.Conclusions: The novel quantitative measurement of IA can be used to evaluate the severity of diabetic iridopathy (DI) accurately and detect invisible NVI as early as possible to provide a basis for the formulation of personalized treatment for DR patients.
Use of Glycolamidic Ester Link. (G.E.L.) for the Preparation of Protected Peptides.
Authors: Ceccato Marie-Line , Chavanieu Alain , Chenu Jacques , Mendre Christiane , Calas Bernard 
Published: June 2022

Abstract:
In this paper, we report the use of Glycolamidic Ester Link (G.E.L.) to synthesise protected peptides. According to the method of cleavage used (NaOH or hydrazine in DMF), it is possible to generate protected species having a -COOH or a -CONHNH2 group in C-terminal position.
Cleavage of the Amino-Terminal Domain of Cd36 by Hiv-1 Protease
Authors: Greenwalt Dale E. 
Published: June 2022

Abstract:
The HIV-I protease has been found to cleave the integral membrane protein CD36 at two specific sites (residues 43*44 and 67*68) in the amino-terminal region of the bovine protein. Neither cleavage site conforms to previously identified type I or type 2 sites characteristic of HIV-1. Human CD36, which differs only slightly in its amino acid sequence.Was not cleaved by the HIV-1 protease.
Comparative Dynamic Features of Apo and Bound MDM2 Protein
Reveal the Mechanism of Inhibitor Recognition for Anti-Cancer
Activity
Authors: I. El habbash Aisha , El Rashedy Ahmed , Soliman Mahmoud E.S. 
Published: June 2022

Background:
Mouse Double Minute 2 Homolog (MDM2) oncogenic protein is the principal cellular antagonist of the p53 tumor suppressor gene. Restoration of p53 activity by inhibiting the MDM2-P53 interactions at the molecular level has become the cornerstone of cancer research due to its promising anticancer effects. Natural medicinal products possess various chemical structures and represent an essential source for drug discovery. α-Mangostin (AM) and gambogic acid (G250) are plant-derived compounds that showed inhibitory effects on MDM2-P53 interactions in-vitro and in-vivo.

Methods:
Despite the many clinical studies which performed deeper insight about the molecular understanding of the structural mechanisms exhibited by α-Mangostin and Gambogic acid-binding to MDM2 remains critical. In this study, comparative molecular dynamics simulations were performed for each Apo and bound p53 and MDM2 proteins to shed light on the MDM2-p53 interactions and get a better understanding of the inhibition mechanisms.

Results:
Results revealed atomistic interaction of AM and G250 within the MDM2-p53 interaction cleft. Both compounds mediate the interaction between the α-helix motifs of the p53 amino-terminal domain. Which caused a significant separation between orthogonally opposed residues, specifically Lys8 and Gly47 residues of the p53 and MDM2, respectively. Contrasting changes in magnitudes were observed in per-residue fluctuation on AM and G250 (~0.04 nm and ~2.3 nm, respectively). The Radius of gyration (~0.03 nm and 0.04 nm, respectively), C-alpha deviations (~0.06 nm and 0.1 nm, respectively). The phenolic group of AM was found to establish hydrogen interactions with Glu28 and His96 residues of MDM2. The trioxahexacyclo-ring of G250 also forms hydrogen bond interactions with Lys51 and Leu26 residues of MDM2.

Conclusion:
Utilizing the information provided on the inhibitory binding mode adopted by each compound in this study may further assist in the tailored designs for cancer therapeutics.
A Prospective Study on the Incidence and Severity of Paclitaxel-induced
Peripheral Neuropathy in the Indian Population
Authors: Reghu R. , George A.M. , Arya M.A. , Joseph S.K. , Philip A. , Sam K.M. 
Published: June 2022

Background:
Despite the high efficacy rate of paclitaxel, physicians are compelled to
discontinue the regimen due to its prevailing neurotoxicity and myelosuppressive effects, thus not
achieving the desired clinical outcomes. The neurotoxicity studies of paclitaxel have been mostly
performed on upper dose limits (>275mg/m2), and little information is available on lower doses.
Since there is a lack of such studies on the Indian population, the medical professionals are unable to
analyze at what cumulative dose does paclitaxel show maximum severity of peripheral neuropathy.

Methods:
This is a prospective observational study conducted for 1 year in patients undergoing
paclitaxel therapy. These patients were evaluated for the incidence and severity of paclitaxel-induced
peripheral neuropathy during the first 6 cycles using the QLQ-CIPN questionnaire. We also identified
the cumulative dose at which most patients developed peripheral neuropathy and each patient’s
quality of life using EORTC QLQ C30.

Results:
Out of 85 patients, 76 developed peripheral neuropathy during the first 6 cycles. It was
observed that the severity of peripheral neuropathy increased in each cycle of therapy. The overall
quality of life of patients decreased with therapy, and at a cumulative dose of 525mg/m2, most of the
patients (40%) developed symptoms of peripheral neuropathy.

Conclusion:
The incidence and severity of peripheral neuropathy increased with each cycle, leading
to a significant reduction in the quality of life of patients post 6 cycles. Moreover, a high cumulative
dose may limit the paclitaxel therapy.
Deep Eutectic Solvent (DES) Mediated Multicomponent Synthesis of 4-
thiazolidinone-5-carboxylic Acid: A Green Chemistry Approach
Authors: Shaikh Majid , Shaikh Mujahed , Wagare Devendra , Ahmed Sheikh Anis , Sultan Kasim Sayyad 
Published: June 2022

Background:
The 4-thiazolidinone-5-carboxylic acid and its derivatives have diverse
applications in agriculture, industrial and pharmaceutical fields. Therefore, the synthesis of this
heterocyclic compound attracted much attention from researchers with green chemistry protocols.
In this research work, we have introduced the green protocol for the synthesis of 4-thiazolidinone-
5-carboxylic acid by keeping the parameters in mind like cost-effective, environmentally benign,
short reaction time and easy work-up procedure.

Methods:
Initially, we irradiated the mixture of substituted aldehyde, thiosemicarbazide and furan
2-5-dione in the presence of choline chloride-thiourea-based Deep Eutectic Solvent [DES] as a
green medium. The reaction optimization was performed in different solvents like ethanol, glycerol,
and PEG-400.

Results:
The DES, which was used as a green solvent, produced an excellent result in context to
short reaction time, yield, easy workup, mild reaction condition and cost-effective protocol. All the
results are discussed.

Conclusion:
The DES-mediated synthesis of 4-thiazolidinone-5-carboxylic acid is found to be an
excellent protocol, which followed green chemistry principles. This method has specific features
like mild reaction conditions, environmentally benign, cost-effective and easy workup procedure.
Restriction Mapping of <i>MC4R</i> Gene on Bali Cattle (<i>Bos sondaicus</i>) as Genetic Marker for Breeding Program in Compared to <i>Bos taurus</i> and <i>Bos indicus</i>
Authors: Perdana Yoga Cipta , Hartatik Tety 
Published: June 2022

MC4R is a gene that has potential effects on growth traits such as body weight and feed intake. The usage of single nucleotide polymorphism (SNPs) in melanocortin-4 receptor (MC4R) as selection markers could help achieve effectiveness in the breeding program. This study aimed to analyze the restriction mapping based on SNPs in the MC4R gene for Bali cattle (Bos sondaicus) compared to various breeds of cattle. Partial MC4R gene was amplified using a primer (F: 5’-ACC AAT GTC AGT GAG TCC CC- 3’ and R: 5’-CTT CAT GTT GGC GCC CTG-3’) with a polymerase chain reaction (PCR) method. Genotype and allele frequencies were calculated using Chi-Square test and analyzed with Hardy–Weinberg law. Restriction enzyme was analyzed using Nebcutter V.2 to see the association between SNPs and the recognition site of restriction enzyme. The result showed four SNPs g.554 T>C, g.634 G>T, g.673 C>T, and g.742 G>A were found in the exon region. SNP g.742 G>A was found as a heterozygote genotype and the rest are SNP g.554 T>C, g.634 G>T, and g.673 C>T were found as homozygote genotypes. All SNPs were synonymous which did not change the amino acid translated. Three restriction enzymes were identified as MmeI, TspRI, and BsrI which attach to SNPs g.554 T>C, g.634 G>T, and g. 742 G>A respectively. SNPs found notably g. 742 G>A can be used as genetic markers associated with growth traits for further research on Bali cattle.
Geriatric Assessment in Elderly People by Using ABCDE (Ability Balance Cognitive Diseases Emotion) Method at Posyandu for Elderly People
Authors: Sulistyaningsih Nunik , Claramita Mora , Pramantara I Dewa Putu 
Published: June 2022

Background: Elderly populations have increased in line with the increased life expectancy. Currently, the balance of disease burden on the elderly has shifted towards chronic disease. Cardiovascular disease, diabetes, cognitive impairment and depression are chronic diseases that cause suffering and death in elderly populations. Therefore, it is necessary to conduct a comprehensive and multidimensional health assessment as a preventive effort to reduce morbidity in the elderly.Objective: The general objective of this research was to obtain a description of elderly health status at the Posyandu for the elderly using a geriatric assessment called the ABCDE (Ability, Balance, Cognitive, Diseases, Emotion) method.Methods: This research used a cross-sectional design. The subjects were elderly persons ≥ 60 years old who participated in the Posyandu for the elderly and who had fulfilled inclusion and exclusion criteria. The estimated minimum sample size was 242 selected using stratified random sampling. The statistical analysis in this research employed univariate, and bivariate analysis using Chi square tests.Results: Elderly participants had light dependency (34.2%) with average activities of daily living (ADL) value of 19.39 ± 1.32, positive Romberg test 3.7%, and Sharpened Romberg Test 50%. The prevalence of cognitive impairment was 43.4%, while the average score of Mini Mental State Examination was 23.31 ± 6.08, hypertension prevalence 73.14%, hypercholesterolemia 13.22%, and diabetes mellitus 10.33%. Elderly who had depression and probable depression were 12.81%, consisting of mild depression 11.57% and moderate depression 1.24%. Balance disorders had a statistically significant correlation with ADL (p = 0.012; Ratio Prevalence (RP) = 1.422: 95% CI), cognitive impairment (p = 0.039; RP = 1.31: 95% CI), and depression (p = 0.034; RP = 1.63: 95% CI).Conclusion: The prevalences of hypertension, cognitive impairment, and balance disorder on sharpened Romberg test were high. There were statistically significant correlations between balance disorder and ADL, cognitive function, and depression. These findings support the application of geriatric assessment by using ABCDE which can be applied in primary care setting for elderly people.
Making and Validating a Promotive and Preventive Effort Model for Stages 1-5 of Chronic Kidney Disease in Primary Care Services
Authors: Hajam Anita Rahman , Djarwoto Bambang , Claramita Mora 
Published: June 2022

Background: The prevalence of Chronic Kidney Disease (CKD) is continuously increasing worldwide, including in Indonesia and has become a global public health problem. With the increased number of patients with CKD and the limited number of nephrologists, primary care physicians and specialists should play an important role in providing quality sustainable prevention and treatment by improving promotive and preventive efforts in primary care. Most patients with CKD can be managed by doctors in primary care in collaboration with specialist doctors in secondary and tertiary care. However, clinical practice guidelines for promoting and preventing CKD in primary care apparently have not been optimally implemented.Objective: This research aimed to make a promotive and preventive model for stages 1-5 of CKD in primary care.Methods: This research employed a research and development strategy with the following stages: (1) Preliminary study stage (literature review), (2) Development stage (initial draft of the model) and (3) Resources validation stage (in-depth interviews).Results: Five primary care physicians, two internists and one nephrologist participated in the validation stage for the initial draft of the model. The three stages of this research obtained the pattern of doctor's practice for CKD and the feedback related to the model to be adjusted according to the condition of primary care in Indonesia.Conclusion: There is a lack of understanding and awareness of primary care physicians about prevention and treatment of CKD, and a lack of supporting examination facilities for serum creatinine and albuminuria in primary care for screening of the risk factors of CKD. A promotional and preventative model of stages 1-5 for CKD in primary care was successfully made and validated.
DEVELOPMENT OF CREATIVE ABILITIES THROUGH ARTS EDUCATION
Authors: Stojanović Stosic Milena 
Published: June 2022

The paper examines possibilities for enhancing creativity in primary school children through Arts education. Specifically, this study investigates the impact of visual, auditory and tactile stimuli (VAT stimuli)[1] on the development of creative thinking in children when these aids are carefully selected and suitable for use for a particular teaching topic within the Arts education curriculum. The study was conducted in Serbia, through an experimental two-term programme called The Development of Creativity by Employing VAT Stimuli, which included 270 first and second-year primary school pupils (7-8 year-olds). The impact of the experimental programme was assessed according to the Torrance Test of Creative Thinking (TTCT) – figural form A. This study found that the use of VAT stimuli in Arts education contributed significantly to developing and cultivating pupils’ creative abilities, and that the effect of the programme had a stronger impact on the 2nd year pupils.[1] Visual stimulus – discussion and observation of an artwork as a stimulus for artistic expression; Auditory aid – instrumental composition as a stimulus for artistic expression; Tactile stimulus – finger painting.
OPTIMALISASI FUNGSI KELUARGA DALAM PENCEGAHAN FENOMENA KEHAMILAN YANG TIDAK DIINGINKAN PADA REMAJA
Authors: Nurhandayani Fitri 
Published: June 2022

Abstract Adolescence is a transition from childhood to adulthood. The role and function of the family is very important for adolescence. Lack of attention and supervision from parents during adolescence will cause problems, such as the occurrence of unwanted pregnancies. In Indonesia, this phenomenon is often referred to as Married By Accident (MBA) or a marriage that occurs due to an unwanted pregnancy. The purpose of this study was to describe the optimization of family functions in preventing the phenomenon of unwanted pregnancy in adolescents. The research theories used are structural functional theory, ecological theory and community education theory. The research method used is a qualitative approach with a descriptive method. Data collection techniques through observation, interviews and documentation. The main reason for using this instrument is to observe the phenomenon of problems faced by families related to optimizing family functions in preventing unwanted pregnancies in adolescents and to obtain research data as material for analysis. The results of this study describe the optimization of family functions on adolescent growth and development and psychological maturity so as to avoid the impact of unwanted pregnancy cases. The conclusion of this study is that the role of the family greatly affects the maturity of adolescents, both young women and young men.
2D Metal-Organic Frameworks Based Optoelectronic Neuromorphic Transistors for Human Emotion-Simulation and Neuromorphic Computing
Authors: Liu Dapeng , Shi Qianqian , Zhang Junyao , Tian Li , Xiong Lize , Dai Shilei , Huang Jia 
Published: June 2022

Two-dimensional metal-organic frameworks (2D-MOFs) have been extensively
studied as promising materials in the fields of eletrocatalysis, drug
delivery, electronic devicese, etc. However, few studies have explored
the application potential of 2D-MOFs in novel neuromorphic computing
devices. In this work, we report an optoelectronic neuromorphic
transistor based on a 2D-MOFs/polymer charge-trapping layer. We found
that, the large specific surface area, stable crystal structure, and
highly accessible active sites in 2D-MOFs make them excellent
charge-trapping materials for our devices, which are beneficial for
mimicking the memory and learning functions observed in the organism’s
nervous systems. Different types of synaptic behaviors have been
realized in our 2D-MOFs-based neuromorphic devices under stimuli signal,
e.g., paired-pulse facilitation, excitatory post-synaptic current,
short-term memory, and long-term memory. More interestingly,
emotion-adjustable learning behavior was realized by changing the value
of the source-drain voltage. This work can shed light on the application
of 2D-MOFs in neuromorphic computing and will contribute to the further
development of neuromorphic computing devices.

Corresponding authors
Email: huangjia@tongji.edu.cn (Jia
Huang)
1610419@tongji.edu.cn (Shilei
Dai)
Diabetes distress, depressive symptoms, and anxiety symptoms in people with type 2 diabetes: A network analysis approach to understanding comorbidity
Authors: McInerney Amy M , Lindekilde Nanna , Nouwen Arie , Schmitz Norbert , Deschênes Sonya S 
Published: June 2022

<p> </p>
<p>Objective</p>
<p>This study aimed to explore interactions between individual items assessing diabetes distress, depressive symptoms, and anxiety symptoms in a cohort of adults with type 2 diabetes using network analysis.</p>
<p>Research design and methods</p>
<p>Participants (<em>N</em> = 1,796) were from the Evaluation of Diabetes Insulin Treatment (EDIT) study from Quebec, Canada. A network of diabetes distress was estimated using the 17 items of the Diabetes Distress Scale (DDS-17). A second network was estimated using the 17 DDS-17 items, the 9 items of the Patient Health Questionnaire, and the 7 items of the Generalized Anxiety Disorder Assessment. Network analysis was used to identify central items, clusters of items, and items that may act as bridges between diabetes distress, depressive symptoms, and anxiety symptoms. </p>
<p>Results</p>
<p>Regimen-related and physician-related problems were amongst the most central (highly connected) and influential (most positive connections) in the diabetes distress network. <em>Failure</em> (depressive symptom) was found to be a potential bridge between depression and diabetes distress, being highly connected to diabetes distress items. The anxiety symptoms of <em>worrying too much, uncontrollable worry, </em>and<em> trouble relaxing</em> were identified as bridges linking both anxiety and depressive items, and anxiety and diabetes distress items, respectively.</p>
<p>Conclusions</p>
<p>Regimen-related and physician-related diabetes-specific problems may be important in contributing to the development and maintenance of diabetes distress. Feelings of failure and worry are potentially strong candidates for explaining comorbidity. These individual diabetes-specific problems and mental health symptoms could hold promise for targeted interventions for people with type 2 diabetes.</p>
New evidence of central nervous system damage in diabetes mellitus: impairment of fine visual discrimination
Authors: Chen He , Wang Menghan , Xia Lin , Dong Jiong , Xu Guangwei , Wang Ziyi , Feng Lixia , Zhou Yifeng 
Published: June 2022

<p> </p>
<p>Diabetes can damage both the peripheral sensory organs, causing retinopathy, as well as the central visual system, leading to contrast sensitivity and impaired color vision in patients without retinopathy. Orientation discrimination is important for shape recognition by the visual system. Our psychophysical findings in this study show diminished orientation discrimination in diabetic patients without retinopathy. To reveal the underlying mechanism, we established a diabetic mouse model and recorded in vivo electrophysiological data in the dorsal lateral geniculate nucleus (dLGN) and primary visual cortex (V1). Reduced orientation selectivity was observed in both individual and populations of neurons in V1 and dLGN, which increased in severity with disease duration. This diabetes-associated neuronal dysfunction appeared earlier in the V1 than dLGN. Additionally, neuronal activity and signal-to-noise ratio is reduced in V1 neurons of diabetic mice, leading to a decreased capacity for information processing by V1 neurons. Notably, the V1 in diabetic mice exhibits reduced excitatory neuronal activity and lower levels of phosphorylated mammalian target of rapamycin (mTOR). Our findings show that altered responses of both populations and single V1 neurons may impair fine vision, thus expanding our understanding of the underlying causes of diabetes-related impairment of the central nervous system.</p>