bluuebunny/crossref_metadata_2025_split huggingface dataset:
Near Infrared Ray-Guided Surgery Using Firefly Technology of the daVinci Xi System and Intraoperative Upper Gastrointestinal Endoscopy for Subtotal Gastrectomy and Surgery for Cancer of the Gastroesophageal Junction
Authors: Sagawa Hiroyuki , Saito Masaki , Ito Sunao , Hayakawa Shunsuke , Ueno Shohei , Okubo Tomotaka , Tanaka Tatsuya , Takahashi Hiroki , Matsuo Yoichi , Mitsui Akira , Kimura Masahiro , Takiguchi Shuji 
Published: March 2022

Abstract
Background
In gastrectomies, especially subtotal gastrectomies and operations on the gastroesophageal junction, identifying the exact location of the tumor and establishing the appropriate resection line is very important. Accurate resection lines have a major impact on the preservation of organ function and curability. Preservation of as much as possible of the remaining stomach, including the fornix, may be an important surgical goal for maintaining an adequate postoperative quality of life. In adenocarcinoma of the gastroesophageal junction, the height of the esophageal dissection may affect reconstruction of the transhiatal approach.
Methods
We perform a new technique, near infrared ray-guided surgery, for the accurate localization of a tumor using the Firefly technology of the daVinci Xi system and intra-operative upper gastrointestinal endoscopy. We used this new technique for cases of upper gastric cancer or adenocarcinoma of the gastroesophageal junction. In this retrospective study, we examined to determine the extent (mm) of the tumor invasion of the esophagus, visualization of near infrared ray contained within endoscopic light, and distance from the proximal margin of the tumor to the surgical cut line on rapid histopathology and in the permanent preparation, including the operative videos and extracted specimens.
Results
We performed near infrared ray-guided surgery for 12 patients with gastric cancer or adenocarcinoma of the gastroesophageal junction, and the near infrared ray was clearly seen as green light with Firefly mode in all the patients. Near infrared ray-guided surgery was useful for obtaining localization of the tumor. In addition, it was possible to resect organ with adequate margins from tumor. Rapid intraoperative histopathological examinations confirmed that the resected specimens had negative margins. None of the patients required additional resection.
Conclusions
We believe that because near infrared ray-guided surgery can provide an accurate resection line, it will be useful for the resection of upper gastric cancer and adenocarcinoma of the gastroesophageal junction. It will also provide patients with a good postoperative quality of life after surgery.
Regulatory Effects of Survivin on Autolysosome Formation-Related Proteins in Esophageal Carcinoma Cells
Authors: Li Hui , LIU LING , Aiziti Palidan , Zhu Shimao , Wusiman Shabahaiti , Yang Yinyin , Wang Yongchen , Tuerxun Hainisayimu , Yi Na , Li Huiwu 
Published: March 2022

Abstract
Purpose:To investigate the regulatory effect of Survivin on autolysosome formation-associated proteins in esophageal cancer cell (ESCC) line Eca109.Methods: An autophagy induction model was constructed using rapamycin (RAPA), an autophagy inhibitor. The Survivin overexpression vector, shRNA vector, and Survivin inhibitor YM155 were used to interfere with the autophagy model. Laser copolymer microscopy technology was utilized to observe the changes in autophagosomes and their progression, while western blotting was used to detect the the level of lysosome formation-related proteins after autophagy. Besides, immunofluorescence staining was used to determine the pH of lysosomes and the distribution of cathepsin-D in cells.Results: (1)Eca109 cells transfected with MRFP-EGFP-LC3 vector by RAPA showed a significant increase in green fluorescence, indicating an increase in autophagosomes. Results from western blotting indicated a rise in protein expression levels of LC3-I and LC3-II, an increase in the ratio of LC3-II/LC3-I, and a decrease in the expression of p62 levels, indicating enhanced autophagy. (2)In the autophagy model of Eca109 cells, after Survivin overexpression, LC3 expression increased, ATG5 expression decreased(P<0.05). After YM155 intervention, ATG5 and ATG16L1 expression rose(P<0.05). (3)In the autophagy model of Eca109 cells, Rab7 and ATG5 expression increased,while ATG16L1 expression decreased(P<0.05) after YM155 intervention and Survivin shRNA transfection. In the presence of the Survivin overexpression vector, YM155 interfered with the expression of ATG5, ATG16L1, and Rab7 in the autophagy model(P<0.05).(4)Decreased Survivin can reduce p62 expression and increase autophagy.(5)Survivin overexpression increased the number of intracellular lysosomes and was involved in the regulation of lysosomal pH changes. Besides, inhibition of Survivin expression resulted in the extravasation of cathepsin-D, which was dispersive and affected the permeability of lysosomes.Conclusion: Survivin expression was directly related to the expression of the autophagy-associated protein LC3 and P62.Survivin is associated with the autophagic process in cancer cells at specific periods, which indicates that Survivin is involved in both early autophagy formation and the later stages of autolysosomal function implementation.
Targeted immunosuppression enhances repeated gene delivery
Authors: Chen Maria , Kim Boram , Jarvis Maria , Fleury Samantha , Deng Shuyun , Nouraein Shirin , Butler Susan , lee Sangin , Chambers Courtney , Hodges H. Courtney , Szablowski Jerzey , Suh Junghae , Veiseh Omid 
Published: March 2022

Abstract
Adeno-associated virus (AAV) vector-based gene therapies can be applied to a wide range of diseases. AAV expression can last for months to years, but vector re-administration may be necessary to achieve life-long treatment. Unfortunately, immune system response against these vectors is potentiated after the first administration, which prevents the clinical use of repeated administration of AAVs. Reducing immune response against AAVs while minimizing immunosuppression would improve gene delivery efficiency and long-term safety. In this study, we quantified the contributions of multiple immune system components towards AAV response in mice. We identified B-cell-mediated immunity as a critical component preventing vector re-administration. Specifically, we found that IgG depletion was insufficient to enhance re-administration, suggesting the key role of B-cell mediated IgM antibodies in the immune response against AAV. Further, we also found that AAV-mediated transduction is improved compared to wild-type mice in µMT mice that lack functional IgM heavy chains and cannot form mature B-cells. Combined, our results suggest that IgM production in B cells is a potential target for therapeutics enabling AAV re-administration. Our results also suggest that the µMT mice are a potentially useful experimental model for gene delivery studies since they allow for up to 15-fold more efficient gene delivery.
Serum lactate dehydrogenase predicts brain metastasis and survival in limited stage small-cell lung cancer patients treated with thoracic radiotherapy and prophylactic cranial irradiation
Authors: JianJiang Liu , Dongping Wu , Bin Shen , Mengyuan Chen , Xia Zhou , Peng Zhang , Guoqin Qiu , Yongling Ji , Xianghui Du , Yang Yang 
Published: March 2022

Abstract
Background: Small cell lung cancer (SCLC) is characterized with high risk of brain metastasis and poor survival. This study aimed to assess the prognostic role of LDH in limited stage small-cell lung cancer (LS-SCLC) treated with thoracic radiotherapy (TRT) and prophylactic cranial irradiation (PCI).Methods: This study retrospectively evaluated 197 consecutive patients who underwent TRT and PCI for LS-SCLC between November 2005 and October 2017. Both pretreatment and maximal serum LDH levels (mLDH) during treatment were checked, increased LDH level was defined as more than 240 IU/ml .Clinical factors were tested for associations with intracranial progression-free survival (IPFS) and overall survival (OS) after PCI. The Kaplan–Meier method was used to calculate survival rates, and multivariate Cox regression analyses were carried out to identify variables associated with survival.Results: Of the total patients, 95 had higher pretreatment LDH levels, and serum LDH levels were increased in 95 patients during treatment. In patients with the normal and elevated mLDH groups, the 1-, 2- and 5-year IPFS rate were 96.7% vs. 90.1%,91.7% vs. 73.8% and 87.8% vs. 61.0% (P < 0.01),respectively. Compared to those with normal LDH level, patients with increased mLDH level had higher cumulative risk of intracranial metastasis [hazard ratio (HR),3.87; 95% confidence interval (CI), 1.73-8.63; P< 0.01], and worse overall survival [HR,2.59; 95% CI, 1.67-4.04; P < 0.01]. The factors of LDH level at baseline or changes between pretreatment level and maximum level during treatment failed to predict BMs or OS with statistical significance.In the multivariate analyses, both mLDH during treatment [HR,3.53; 95% CI, 1.57-7.92; P = 0.002] and age≥60 [HR, 2.46; 95% CI,1.22-4.94; P =0.012] were independently associated with worse IPFS. Factors significantly associated with worse OS included mLDH during treatment [HR, 2.45; 95% CI, 1.56-3.86; P< 0.001], IIIB stage [HR, 1.75; 95% CI,1.06-2.88; P =0.029] and conventional radiotherapy applied in TRT [HR, 1.66; 95% CI,1.04-2.65; P =0.034]. Conclusion: mLDH level during treatment, but not pretreatment level predicts brain metastasis and survival in LS-SCLC patients treated with TRT and PCI, which may provide valuable information for identifying patients with poor survival outcomes.
Simultaneous catalytic oxidation of elemental mercury and arsine over CeO2(111) surface: A density functional theory study
Authors: Zhang Yingjie , Yu Huijuan , Wang Xueqian , Wang Langlang , Li Yuancheng , Lv Dongpeng , Zhu Dan , Tian Chunmei 
Published: March 2022

Abstract
Ceria (CeO2) based materials are potential catalysts for the removal of the Hg0 and AsH3 present in reducing atmospheres. However, theoretical studies investigating the Hg0 and AsH3 removal capacity of ceria remain limited. In this study, the adsorption behavior and mechanistic pathways for the catalytic oxidation of Hg0 and AsH3 on the CeO2(111) surface, including the calculation of optimized adsorption configurations and energies, were investigated using density functional theory calculations. The results suggest that Hg0 and AsH3 are favorably adsorbed on the CeO2(111) surface, whereas CO is not, which is crucial for selective removal when CO is a desirable gas component. Further, AsH3 is adsorbed more favorably than Hg0. In addition, the calculations revealed that the Hg atom is initially adsorbed on the surface and then oxidized by lattice oxygen to form HgO. Concerning AsH3 decomposition, the stepwise dehydrogenation of AsH3 followed by bonding with lattice O atoms to form As-O bond seems the most plausible. Finally, the adsorbed As-O bond is further formed elemental As and As2O3. Therefore, CeO2 can adsorb and remove Hg0 and AsH3, making it a promising catalyst for the simultaneous catalytic oxidation of Hg0 and AsH3 in strongly reducing off-gas.
Research through action: research protocol on the implementation of an integrated place-based primary intervention in a multicultural context.
Authors: Maillet Lara , Manceau Luiza Maria , Desjardins France , Gagnon Suzanne , Naraziah Lavanyah , Malham Sabina Abou 
Published: March 2022

Abstract
Our project specifically aims at improving the adaptation capacity of primary services in order to answer the complex needs of vulnerable people. The aim is to co-build, with our collaborators and partners, environment-based strategies enabling the coordination of primary services offered by health, social and community responders all the while ensuring the continuity of care (informational, relational and contact) of vulnerable people, especially refugees. A particular attention is brought toward the experiential knowledge of the health system users. This is why at least three citizen-partners are part of the research team since the beginning of the project.Goals: This research-action aims at implementing a “meshing innovation”, meaning an intervention that is integrated, lasting and based on the needs of a community located in a Sherbrooke neighbourhood in order to improve the care of refugee people in a continuum of primary services. To reach this goal, we have 5 specific objectives. The overall goal of this project is to improve the services trajectory in the health and social services network for refugee people in Quebec. The implementation of a meshing innovation, through this pilot project, is a first step toward this overall goal. Method: This project falls within a participative research approach, based on the symmetry principle of the researchers and field stakeholders’ knowledge. To do so, we choose three distinct sources of data: (1) literature review, (2) focus group (N=9 twice), and (3) semi-structured interviews (N=10).Expected results: Concretely, an Implementation guide of establishment strategies for integrated interventions enabling the improvement of primary care for refugees will be written and will constitute the basis for an appropriation workshop for the members of the two joint research networks focused on primary practices (RRAPPL) (Université de Laval and Université de Sherbrooke) and, eventually, in other fields interested in adapting and implementing these strategies. In addition to this guide, we wish to facilitate the care of refugees by family practitioners of the territory; this is why many of them are part of the project. Explicit ties will help raising awareness toward other family practitioners working in medical clinics of the territory while trying to facilitate a specific attribution to these practitioners through the Quebec Family Doctor Finder (GAMF). With this project, we strongly hope to promote the vulnerability factors of refugees to the Health and Social Services Department (MSSS) and to the Quebec General Practitioners Federation (FMOQ) in order to change the priority at the GAMF. Finally, a scientific publication will be written for an international journal.
Mitochondrial C1QBP Is Essential for T Cell Antitumor Function by Maintaining Mitochondrial Plasticity and Metabolic Fitness
Authors: Zheng Junnian , Tian Hui , Wang Gang , Wang Qiping , Jiang Guan , Li Huizhong , Chai Dafei , Song Jingyuan , Fang Lin , Wang Meng , Guo Zengli 
Published: March 2022

Abstract
The metabolic stress present in tumors microenvironment (TME) attenuates T cells anti-tumor capacity, whereas the immune function of T cells is intrinsically controlled by their mitochondrial plasticity including mitochondrial dynamics, metabolism and biogenesis. Previous studies have reported that the complement C1q binding protein (C1QBP), a mitochondrial protein, is responsible for maintenance of the mitochondrial integrity and fitness in dendritic cells and tumor cells. However, its role in T cells, especially in T cells mediated anti-tumor immunity, remains unclear. Here we show that C1QBP is required to maintain T cell anti-tumor immunity by regulating mitochondrial biogenesis, dynamics and metabolic fitness even when only one allele of C1qbp is deleted, without affecting T cells development and homeostasis. Further analysis of C1QBP in the chimeric antigen receptor (CAR) T cell therapy against B16 melanoma model confirmed the cell intrinsic role of C1QBP in regulating T cells antitumor function. Mechanistically, we found C1QBP regulated mitochondrial plasticity and metabolic fitness through mitochondrial biogenesis via the AMP-activated protein kinase (AMPK)/peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) signaling pathway, as well as mitochondrial morphology via the phosphorylation of mitochondrial dynamics protein dynamin-related protein 1 (Drp1). In summary, our study provides a novel mitochondrial target to reprogram the immune metabolism of T cells and improve their immunotherapeutic potential against tumors.
Impact of COVID-19 on carers of people with dementia in the community: Findings from the IDEAL cohort
Authors: Quinn Catherine , Gamble Laura D. , Parker Sophie , Martyr Anthony , Collins Rachel , Victor Christina , Dawson Eleanor , Hunt Anna , Pentecost Claire , Allan Louise , Clare Linda 
Published: March 2022

Abstract

Background
Unpaid carers for people with dementia play a crucial role in society. Emerging evidence suggests the COVID-19 pandemic has negatively impacted on carers.
Objective
To explore the impact of the COVID-19 pandemic on carers for community-dwelling people with dementia and compare responses with pre-pandemic data.
Methods
Data were collected between September 2020 and April 2021 in England and Wales. Carers were identified from the IDEAL cohort and data were collected either through the telephone, video conferencing or an online questionnaire. Responses from 242 carers were compared against benchmark data from the IDEAL cohort. Analyses were conducted for the full sample of carers and for the spousal/partner carers only.
Results
48.8% of carers identified their healthcare needs were negatively affected during the pandemic. Compared with pre-pandemic data carers were more optimistic and had higher social contact with relatives. There were changes in the methods carers used for contacting relatives and friends. Carers were more lonely and experienced less life satisfaction. Most carers coped very or fairly well during the pandemic. There was little impact on carers’ experience of caregiving, although carers felt trapped by the person’s dementia. There was little difference in the experiences of spousal/partner carers and the full sample.
Conclusions
To some extent carers were coping with the pandemic but clearly would benefit from support in terms of alleviating feelings of loneliness and increasing life satisfaction. Services need to consider how to improve access to health care under conditions such as those experienced during the pandemic.
Health Related Quality of Life of Patients Undergoing In-Centre Hemodialysis in Rwanda: a Cross Sectional Study
Authors: SHUMBUSHO Gloria , HATEGEKA Celestin , Vidler Marianne , KABAHIZI Jules , MCKNIGHT Marla 
Published: March 2022

Abstract
Background
There are few studies assessing the quality of life of patients with chronic and end stage kidney disease in sub-Saharan Africa. We aimed to describe the health-related quality of life (HRQOL) of patients undergoing in-centre maintenance hemodialysis in Rwanda using the KDQOL™-36 and determine sociodemographic and clinical factors associated with their quality of life.
Methods
We conducted a multicenter, cross-sectional study between September 2020 and March 2021. Patients over the age of 18 receiving maintenance in-centre hemodialysis for at least 3 months at the Rwandan tertiary hospitals were administered the KDQOL™-36 questionnaire to assess physical and mental health functioning, the effect, burden and symptoms and problem of kidney disease. Sociodemographic and clinical information were also collected for all eligible patients. Using mixed effects linear regression models, we explored factors associated with overall KDQOL and its domains, while accounting for clustering of patients within hemodialysis centres.
Results
Eighty-nine eligible patients were included in the study. The majority of participants were younger than 60 years old (69.7%), male (66.3%), had comorbidities (91%), and 71.6% were categorized as level 3 on a 4 tier in-country poverty scale. All participants had health insurance coverage, with 67.4% bearing no out of pocket payments for hemodialysis. The mean (SD) quality of life score was 48.92 (18.84) for overall HRQOL, 37.33 (10.66) for PCS and 44.74 (9.98) for MCS. Symptoms and problem of kidney disease, effect of kidney disease, and burden of kidney disease scored 58.22 (27.44), 53.48 (17.14) and 20.01 (18.27), respectively. A notable difference of KDQOL scores between hemodialysis centres was observed. Overall KDQOL was associated with male sex (aß [adjusted ß coefficient]: 8.54, 95% confidence interval [CI]: 2.77, 14.26); being employed (aß: 8.16, 95% CI: 2.18, 14.29); dialysis vintage of 13–24 months (aß: 10.47, 95% CI: 3.57, 17.58), hemoglobin of 10-11g/dl (aß: 7.27, 95% CI: 0.70, 13.72) and comorbidities (e.g., 4 comorbidities vs none (aß: -29.76, 95% CI: -41.47, -18.32).
Conclusions
Patients on in-centre hemodialysis in Rwanda have reduced KDQOL scores, particularly in the burden of kidney disease and physical composite summary domains. Higher overall KDQOL mean score was associated with male sex, being employed, and dialysis vintage of 13–24 months, hemoglobin of 10-11g/dl and absence of comorbidities. Majority of patients have higher socioeconomic status reflecting the social and financial constraints to access and maintain dialysis in resource limited settings.
Molecular Interaction of Novel Phytocompounds From Smilax Zeylanica (L) Against the Targeted Protein of Mosquito Vectors and Their Potential Activity
Authors: Balamurugan Chandramohan , Murugan Kadarkarai , Pari Madhiyazhagan , Sivashanmugam Preethi , Kumar Praveenkumar , Thangamani Lokesh , Piramanayagam Shanmughavel 
Published: March 2022

Abstract
Malaria, dengue fever, filariasis, and viral encephalitis are all mosquito-borne illnesses that cause a great number of health issues across the world. Mosquitoes inject saliva into their hosts' skin to make obtaining a blood meal simpler by inhibiting hemostasis. D7 proteins are abundant in mosquito saliva and act as biogenic amine and eicosanoids scavengers. The chemical interactions of possible Smilax zeylanica compounds with mosquito-targeted D7 salivary protein and odorant binding protein were investigated. From petroleum ether, acetone, and ethanolic solvents, a total of 28 chemical components were discovered in the GC-MS study of S . zeylanica . Different solvents were also tested for their adulticidal and repellent properties against Anopheles stephensi, Aedes aegypti , and Culex quinquefasciatus . Among the three solvents, the ethanolic extract exhibited the highest toxic level against the three mosquito species. LC 50 of P. ether 284.92ppm, 311.97ppm and 332.76ppm; LC 50 of acetone 241.97ppm, 271.61ppm and 304.99ppm; and LC 50 of ethanol 217.12ppm, 241.89ppm and 266.90ppm, against the three mosquito species, respectively. In repellent activity from all the solvents, complete protection was observed for up to 150 minutes against the three mosquito species. A total of 22 compounds were taken for docking studies. The compounds were subjected to docking against the three mosquitoes' D7protein. Overall, this study highlights the importance of a computational approach based on control of mosquito vectors and potential activity of biopesticides.