bluuebunny/crossref_metadata_2025_split huggingface dataset:
The emotional impact of disrupted environmental contexts: Enrichment loss and coping profiles influence stress response recovery in Long–Evans rats
Authors: Kent Molly , Kovalev Dmitry , Hart Benjamin , Leserve Danielle , Handford Gabriella , Vavra Dylan , Lambert Kelly 
Published: June 2022

AbstractWith increasing rates of anxiety and mood disorders across the world, there is an unprecedented need for preclinical animal models to generate translational results for humans experiencing disruptive emotional symptoms. Considering that life events resulting in a perception of loss are correlated with depressive symptoms, the enrichment‐loss rodent model offers promise as a translational model for stress‐initiated psychiatric disorders. Additionally, predisposed temperament characteristics such as coping styles have been found to influence an individual's stress response. Accordingly, male rats were profiled as either consistent or flexible copers and assigned to one of three environments: standard laboratory housing, enriched environment, or enriched environment exposure followed by downsizing to standard laboratory cages (i.e., enrichment‐loss group). Throughout the study, several behaviors were assessed to determine stress, social, and reward‐processing responses. To assess recovery of the stress response, fecal samples were collected following the swim stress in 3‐h increments to determine the recovery trajectory of corticosterone (CORT) and dehydroepiandrosterone (DHEA) metabolite levels. Upon death, neural markers of neuroplasticity including doublecortin, glial fibrillary acidic factor, and brain‐derived neurotrophic factor were assessed via immunohistochemistry. Results indicated the flexible coping animals in the continuous enriched group had higher DHEA/CORT ratios (consistent with adaptive responses in past research); furthermore, the enrichment‐loss animals exhibited a blunted CORT response throughout the assessments and enriched flexible copers had faster CORT recovery rates than consistent copers. Standard housed animals exhibited less exploratory behavior in the open field task and continuous enriched, flexible rats consumed more food rewards than the other groups. No differences in neuroplasticity neural markers were observed. In sum, the results of the present study support past research indicating the disruptive consequences of enrichment‐loss, providing evidence that the model represents a valuable approach for the investigation of neurobiological mechanisms contributing to interindividual variability in responses to changing experiential landscapes.
Cultured dissociated primary dorsal root ganglion neurons from adult horses enable study of axonal transport
Authors: Adalbert Robert , Cahalan Stephen , Hopkins Eleanor L. , Almuhanna Abdulaziz , Loreto Andrea , Pór Erzsébet , Körmöczy Laura , Perkins Justin , Coleman Michael P. , Piercy Richard J. 
Published: June 2022

AbstractNeurological disorders are prevalent in horses, but their study is challenging due to anatomic constraints and the large body size; very few host‐specific in vitro models have been established to study these types of diseases, particularly from adult donor tissue. Here we report the generation of primary neuronal dorsal root ganglia (DRG) cultures from adult horses: the mixed, dissociated cultures, containing neurons and glial cells, remained viable for at least 90 days. Similar to DRG neurons in vivo, cultured neurons varied in size, and they developed long neurites. The mitochondrial movement was detected in cultured cells and was significantly slower in glial cells compared to DRG‐derived neurons. In addition, mitochondria were more elongated in glial cells than those in neurons. Our culture model will be a useful tool to study the contribution of axonal transport defects to specific neurodegenerative diseases in horses as well as comparative studies aimed at evaluating species‐specific differences in axonal transport and survival.
Low‐energy green light alleviates senescence‐like phenotypes in a cell model of photoaging
Authors: Jia Chuanlong , Gong Chengchen , Lu Yongzhou , Xu Nan 
Published: June 2022

AbstractBackgroundUltraviolet B (UVB) affects diverse pathways in skin cells, resulting in skin photoaging. Skin fibroblasts internalize and degrade elastin and collagen, playing prominent roles in photoaging. Green light is used in many fields of dermatology, but few studies have examined its role in photoaging. The present work aimed to assess low‐energy green light for its effects in a previously proposed cell model of photoaging and to explore the possible anti‐photoaging mechanism.MethodsThe stress‐induced premature senescence (SIPS) model was constructed via repeated treatment of MDFs with UVB. Senescence‐like phenotypes were compared among normal, low‐energy green light pretreatment and UVB groups, for example, cell morphological properties, senescence‐associated β‐galactosidase (SA‐β‐gal) amounts, extracellular matrix (ECM) biosynthesis and degradation, and autophagy.ResultsIn comparison with the UVB group, the green light pretreatment group showed significantly decreased number of senescent mast cells and markedly declined signal intensity and amounts of SA‐β‐gal‐positive cells. Furthermore, green light pretreatment directly affected ECM by increasing type I and type III collagen production and decreasing MMP‐1 amounts. Moreover, changes in autophagy levels induced by green light pretreatment provided a potential mechanism underlying its anti‐aging property.ConclusionsLow‐energy green light pretreatment improves senescence‐like phenotypes in vitro, indicating a possible application for anti‐aging in clinic after future research has uncovered the potential mechanism.
Assessing masticatory performance with a colour‐mixing ability test using smartphone camera images
Authors: Schimmel Martin , Rachais Elias , Al‐Haj Husain Nadin , Müller Frauke , Srinivasan Murali , Abou‐Ayash Samir 
Published: June 2022

AbstractBackgroundColour‐mixing ability tests are frequently used to assess masticatory performance, but the image acquisition process may be cumbersome and technique sensitive.ObjectivesTo evaluate the reliability of smartphone camera images in assessing masticatory performance using a colour‐mixing ability test.MethodsParticipants were recruited into three groups of dental state (n = 20 each): fully dentate, removable partial denture wearers and complete denture wearers. After performing a colour‐mixing ability test, images of the gum specimens (Hue‐Check Gum©) were captured with two smartphones and compared with the images obtained from a flatbed scanner by two examiners. The images were analysed with a subjective‐ (SA) and an opto‐electronical assessment (VoH). Inter‐ and intra‐rater reliability were tested. ANOVA models with repeated measures were used for statistical analysis (⍺ = .05).ResultsAll three image acquisition techniques were able to distinguish masticatory performance between different dental states. For SA, inter‐rater reliability was fair to substantial and intra‐rater reliability was substantial to almost perfect. For VoH, inter‐rater reliability with the smartphones was at times different between two examiners, but the intra‐rater assessment was reliable. The opto‐electronic analysis with smartphone images underestimated the masticatory performance significantly when compared to the flatbed scanner analysis. Seven‐day ageing of the specimens did not significantly affect the results.ConclusionsThe assessment of masticatory performance with the Hue‐Check Gum© is a reliable method. The use of smartphones may occasionally underestimate masticatory performance; image acquisition with a flatbed scanner remains the gold standard. A centralised analysis of the photographed wafer may foster the reliability of the diagnosis.
Effect of lentivirus‐mediatedBMP2from autologous tooth on the proliferative and osteogenic capacity of human periodontal ligament cells
Authors: Qin Ruoshan , Cui Ziwei , Zhou Hongli , Guo Ru , Yao Xuanxuan , Wang Tao , Qin Xiaodong , He Xiangyi 
Published: June 2022

AbstractBackground and objectivePeriodontitis is a chronic progressive inflammation that invades periodontal supporting tissues, in which periodontal tissue regeneration engineering offers new hope for prevention and treatment, including seed cells, scaffolds, and growth factors. In recent years, scholars have shown that autologous teeth can be used as new bone tissue repair materials for periodontal regeneration and bone tissue repair. The aim of this study was to establish a human periodontal ligament cell line that expresses the human bone morphogenetic protein 2 gene (BMP2) in a stable manner using lentiviral mediation in order to explore the effect of BMP2 from autologous tooth on the proliferative and osteogenic capacity of human periodontal ligament cells (hPDLCs).Materials and methodsHuman periodontal ligament cells were cultured, subcultured, and identified, and then homologous recombinant lentivirus plasmid plv‐BMP2 was constructed and transfected into the third passage (P3) hPDLCs. After that, the effect of BMP2 on its proliferation was detected by CCK‐8, at the same time, the osteogenic induction of hPDLCs was carried out at 7, 14, and 21 days, and then the effect of BMP2 on its osteogenic ability was detected by alizarin red staining, alkaline phosphatase activity determination, and the mRNA expression levels of osteogenic‐related genes using real‐time fluorescence quantitative PCR, including alkaline phosphatase, runt‐related transcription factor 2, bone sialoprotein, osteocalcin, osteopontin, and collagen I. Finally, spss26.0 software was used for statistical processing.ResultsThe results showed that cells transfected with the homologous recombinant lentiviral plasmid pLV‐BMP2 had a similar morphology to normal hPDLCs, showing a typical radial arrangement; the cell proliferative capacity of the pLV‐BMP2 group as measured by CCK‐8 was enhanced compared with the control group and the pLV‐puro group (p < .05); alizarin red staining and alkaline phosphatase activity assay showed that the osteogenic ability of pLV‐BMP2 was significantly enhanced compared with the control and pLV‐puro groups (p < .01), and the findings of real‐time fluorescence‐based quantitative PCR showed high expression of osteogenic‐related genes in pLV‐BMP2 group (p < .01).ConclusionIn conclusion, a stable periodontal ligament cell line overexpressing BMP2 was successfully established by a lentivirus‐mediated method, which proved that BMP2 has a strong ability to promote the proliferation and osteogenesis of hPDLCs, thereby providing an opportunity for the study of periodontal tissue regeneration as well as providing an experimental basis for the application of autologous teeth as a new type of bone repair material for periodontal therapy and even for maxillofacial bone tissue repair.
Susceptibility to violent extremism and cognitive rigidity: Registered replication, corroboration and open questions for criminological research and practice
Authors: Zmigrod Leor 
Published: June 2022

AbstractDoes cognitive inflexibility predict vulnerability to violent extremism? Schumann, Salman, Clemmow, and Gill (2021) conducted a registered direct replication of Zmigrod, Rentfrow, and Robbins’ (2019, Frontiers in Psychology, 10, 989) studies on the relationship between neuropsychologically assessed cognitive inflexibility and extremist attitudes. Replicating the original study, Schumann et al. (2021) demonstrated that cognitive inflexibility on the Remote Associates Test was related to individuals' willingness to fight and die for their ideological group (Bayes Factor = 58.7). In a further corroboration, the study found that individuals who indicated they would self‐sacrifice in a trolley‐dilemma paradigm were more psychologically rigid on the Remote Associates Test than those who opted for self‐preservation (Bayes Factor = 402.3). A mini meta‐analysis with the original study revealed a significant negative correlation between cognitive flexibility and violent extremist behaviour intentions, thereby supporting the conclusions of the original study by Zmigrod and colleagues (2019). Nevertheless, the replication study highlighted moderators of the effect sizes of the reported relationships and revealed subtleties in the theoretical relationships between cognitive style and tendencies towards extremist attitudes. Follow‐up analyses with Bayesian statistics demonstrated that—corroborating the original study—cognitive inflexibility on both the Remote Associates Test (Bayes Factor = 7.2) and the Wisconsin Card Sorting Test (Bayes Factor = 2.3) are linked to individuals' willingness to fight for an ideological cause. However, the replication study revealed that these effects are specific to individuals who endorse ideological self‐sacrifice. Furthermore, deviating from the original study, Schumann and colleagues found that cognitive flexibility on the Wisconsin Card Sorting Test may in fact positively predict willingness to die for a group under some conditions. This suggests that flexibility may be a double‐edged sword when it comes to ideological conversion. These insights indicate that a fine‐grained examination of risk sub‐profiles and interactions among predictors is essential. The findings of the replication also stimulate a discussion of what constitutes a phenomenologically or practically meaningful effect size for psychological and criminological research and practice—and the implications for cognitive methodologies in risk assessment.
Mitochondrial genomic data are effective at placing mycoheterotrophic lineages in plant phylogeny
Authors: Lin Qianshi , Braukmann Thomas W. A. , Soto Gomez Marybel , Mayer Juliana Lischka Sampaio , Pinheiro Fábio , Merckx Vincent S. F. T. , Stefanović Saša , Graham Sean W. 
Published: June 2022

Summary

Fully mycoheterotrophic plants can be difficult to place in plant phylogeny due to elevated substitution rates associated with photosynthesis loss. This potentially limits the effectiveness of downstream analyses of mycoheterotrophy that depend on accurate phylogenetic inference. Although mitochondrial genomic data sets are rarely used in plant phylogenetics, theory predicts that they should be resilient to long‐branch artefacts, thanks to their generally slow evolution, coupled with limited rate elevation in heterotrophs.

We examined the utility of mitochondrial genomes for resolving contentious higher‐order placements of mycoheterotrophic lineages in two test cases: monocots (focusing on Dioscoreales) and Ericaceae.

We find Thismiaceae to be distantly related to Burmanniaceae in the monocot order Dioscoreales, conflicting with current classification schemes based on few gene data sets. We confirm that the unusual Afrothismia is related to Taccaceae–Thismiaceae, with a corresponding independent loss of photosynthesis. In Ericaceae we recovered the first well supported relationships among its five major lineages: mycoheterotrophic Ericaceae are not monophyletic, as pyroloids are inferred to be sister to core Ericaceae, and monotropoids to arbutoids.

Genes recovered from mitochondrial genomes collectively resolved previously ambiguous mycoheterotroph higher‐order relationships. We propose that mitochondrial genomic data should be considered in standardised gene panels for inferring overall plant phylogeny.
Sawing the branch of near‐death experience research: A critical analysis of Parnia et al.’s paper
Authors: Evrard Renaud , Pratte Erika , Rabeyron Thomas 
Published: June 2022

AbstractIn their recent paper, Parnia and colleagues propose a new label for the near‐death experience (NDE): recalled experience of death. They claimed NDEs are “authentic” only when an objective danger is present and that authentic NDEs have a proven core phenomenology. We consider that these claims are insufficiently supported by empirical data. NDEs appear as a continuum of heterogeneous experiences of consciousness precipitated by the disjunction of processes usually combined in normal mental activity. The “core phenomenology” of NDEs is also opened to several criticisms. Closeness to “real” death does not appear to be a decisive criterion for characterizing NDEs. The author's adhesion to Raymond Moody's NDE model produces a biased partition of this field of research that is unable to provide the basis for a consensus.
Diversity of tolerance to low temperature at seed germination stage in rice (Oryza sativa)
Authors: Fujino Kenji 
Published: June 2022

AbstractCrop cultivation has expanded worldwide where the environmental conditions vary and differ from their origins. Low temperature is a serious stress for rice, which originated in the tropics. This study evaluated tolerance to low‐temperature at the seed germination stage (low‐temperature germinability) in the World Early (WE) population showing early heading date, as it might increase adaptability to low‐temperature conditions during rice growth at high latitudes. Low‐temperature germinability varied widely from 0% to 100% at five days after incubation at 15°C. To estimate the genetic basis of low‐temperature germinability involved in WE population, nine F2 populations (29<n<94) were developed. The patterns of frequency distributions of low‐temperature germinability in these F2 population were classified into three types, Types 1–3. Sequence comparison of gene for low‐temperature germinability, qLTG3‐1 (quantitative trait locus [QTL] for low‐temperature germinability on chromosome 3), identified six alleles. Therefore, the associations between qLTG3‐1 genotype and low‐temperature germinability phenotype suggested that gene(s) other than qLTG3‐1 might be involved in low‐temperature germinability in this population.
Toxic epidermal necrolysis‐like acute graft‐versus‐host disease in pediatric bone marrow transplant patients: Case series and review of the literature
Authors: Sheu Song Johanna , Huang Jennifer T. , Fraile Alonso Maria del Carmen , Antaya Richard J. , Price Harper N. , Funk Tracy , Francois Rony A. , Shah Sonal D. 
Published: June 2022

AbstractBackground/ObjectivesComplications of hematopoietic stem cell transplant (HSCT) include acute graft‐versus‐host disease (aGVHD). Severe cutaneous aGVHD can present with generalized erythroderma, desquamation, and bullae which can mimic toxic epidermal necrolysis (TEN). TEN occurs in response to a culprit medication. Transplant patients are often on many medications, making it difficult to distinguish between the two conditions. Given that TEN‐like aGVHD is rare, we describe a case series of pediatric patients and review the literature.MethodsThis is a multi‐institutional case series of children who developed TEN‐like aGVHD following bone marrow transplantation. Demographic, clinical, and treatment information was collected.ResultsTen patients were identified. Median age at transplantation was 8.5 years (range 0.12–17 years). Median time from transplant to first skin symptoms was 35 days (range 6–110 days) and to first TEN‐like symptoms was 40 days (range 16–116 days). 7/10 had other organ GVHD involvement. All patients were on concurrent medications at time of first skin symptoms including immunosuppression for GVHD prophylaxis, infection prophylaxis or treatment, and pain medication. Treatments for TEN‐like aGVHD included immunosuppression.ConclusionsWe observe that patients with > or equal to 50% BSA involvement of their skin with TEN‐like aGVHD, extracutaneous GVHD, and lack of reepithelization tend to have poor outcomes. Given the rarity of this condition, multidisciplinary care of these patients is important for accurate and timely diagnosis and treatment.
Risk factors for IgG4‐related disease relapse: A systematic review and meta‐analysis
Authors: Yue Zifan , Tong Fei , Chen Yuqing , Shen Ya , Li Jian , Zeng Chengcheng , Diao Jiale , Chen Xinxin , Wei Ruili 
Published: June 2022

AbstractIgG4‐related disease (IgG4‐RD) is an autoimmune disease involving multiple organs with unique pathological features and has high relapse rates after treatment. Identifying patients at high risk of disease relapse for targeted treatment with long‐term and low‐dose glucocorticoid maintenance therapy is crucial for formulating rational treatment strategies. In this study, we conducted a meta‐analysis of relevant articles to explore the risk factors for IgG4‐RD relapse. We searched the Medline (via PubMed), EMBASE, Web of Science and Cochrane Library databases and extracted the mean and standard deviation of continuous variables and true‐positive (TP), false‐positive (FP), false‐negative (FN) and true‐negative (TN) rates to construct a 2 × 2 contingency table from dichotomous variables, and hazard ratios (HRs) were calculated from univariate and multivariate analyses. Finally, we identified five indicators (elevated baseline serum IgG4 level, eosinophil count, increased multiple organ involvement, history of allergy and proximal bile duct stenosis) as risk factors for IgG4‐RD relapse. These results provide new ideas and directions for more researchers to study the relapse of IgG4‐RD and offer reasonable suggestions for clinicians to select IgG4‐RD patients for low‐dose glucocorticoid maintenance therapy to reduce their relapse rates.
Percutaneous doxycycline sclerotherapy in a horse with a mandibular aneurysmal bone cyst
Authors: Perez Jairo A. , Weber Kristy L. , Bender Susan J. , Wulster Kathryn B. , Parente Eric J. 
Published: June 2022

AbstractObjectiveTo describe the use and outcome of sclerotherapy with intralesional doxycycline foam in a horse with a mandibular aneurysmal bone cyst.Study designCase report.AnimalsClient‐owned 1 year old Standardbred filly.MethodsThe horse presented for progressive mandibular swelling. A 10 mg/mL doxycycline foam was prepared for intralesional injection. Three doses were injected into the lesion under computed tomographic guidance at 6 and 15 weeks after initial treatment. Volume reduction was monitored after each treatment with 3D volumetric rendering and region of interest segmentation using commercially available software.ResultsThe volume of the lesion decreased from 458.7455 cm3before treatment, to 363.3101 cm3at 6 weeks, 273.5855 cm3at 15 weeks, and 247.2316 cm36 months later, resulting in a total reduction of 54% of the initial volume. Bone formation was noted in the lesion. No adverse effects related to doxycycline foam injections were noted. The mandibular swelling was resolved after treatment.ConclusionIntralesional doxycycline sclerotherapy was shown to be efficacious in reducing the volume of the aneurysmal bone cyst in the horse presented in this report. There was complete resolution of mandibular swelling with no side effects related to the intralesional injections.
Epicentral influence via agent‐based modelling
Authors: Hundt Marianne , Van Driessche Laetitia , Pijpops Dirk 
Published: June 2022

AbstractOne limitation of corpus‐based research into the epicentre hypothesis is that it only provides information on structural similarity/difference of varieties but not on the role that attitudes may play in the choice of variants in a pluricentric language. In our case study on verb complementation patterns, we use simulation as a complementary methodology. We build an agent‐based model for two speaker communities with English as a second language (ESL) which allows for attitudes towards American and British English to affect the choice of verb complementation. This approach enables us to gauge the likely effect that attitudes towards potential epicentres might have on the choice of variants and to predict patterns of variation we should see in language use. We provide preliminary data from the News On the Web corpus to test whether the predictions are, in fact, borne out.