bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response Time in the Transport of Pediatric Patients to a Tertiary Critical Care Unit
Authors: Villacrés Sindy , Katyal Chhavi , Gomez James , Longani Neha , Chang Deidre , Velasco Susan , Zeiman Linda , Choi Steven 
Published: June 2022

Introduction:
Various barriers delay the process of patient transfer to critical care units. We implemented quality improvement methods to decrease the time required for interhospital transfer of critical care patients. As a result, we aimed to decrease the time from initial transfer call to specialized transport team arrival at the referring hospital from 150 minutes to <40 minutes over 2 years.

Methods:
Quality improvement initiative monitoring the length of transport time of 245 patients transferred from referral hospitals to a tertiary pediatric intensive care unit for 31 months from March 2013 to October 2015. We reviewed preexisting transport protocols and identified barriers to the timely arrival to the pediatric intensive care unit. We implemented 3 interventions: a transport information line serving as a central communication center to coordinate the transport process between all stakeholders, the formation of a specialized pediatric transport team, and a training program. We collected transport response time data and monitored the impact of interventions via statistical process control charts.

Results:
There was a significant decrease in the length of the time course pre- and postintervention. We noted a special cause to decrease in time from referral hospital call to arrival of our transport team by 76% from 150 minutes to 36 minutes. In addition, the statistical process chart revealed a stable and effective process without significant shifts above the process mean as early as 3 months postintervention.

Conclusions:
By improving our transport services with additional resources and people, we have improved the efficiency of patient transport between institutions.
Assessing Suicide Risk in a Pediatric Outpatient Behavioral Health System: A Quality Improvement Report
Authors: Soffer Stephen L. , Lewis Jason , Lawrence O’Nisha S. , Marroquin Yesenia A. , Doupnik Stephanie K. , Benton Tami D. 
Published: June 2022

Introduction:
Standardized suicide risk assessment improves the detection of individuals at risk of suicide. We conducted a quality improvement initiative in a system of outpatient behavioral health practices affiliated with a free-standing children’s hospital to implement standardized suicide risk assessment for new patients.

Methods:
Clinicians received education in suicide risk assessment and were trained to use an evidence-based suicide risk assessment tool, the Columbia Suicide Severity Rating Scale (C-SSRS). We standardized workflow processes and integrated the C-SSRS in the electronic health record with a feature to communicate instances of elevated risk across care teams through a problem list. We analyzed C-SSRS responses and adherence to standardized processes and compared the percentage of patients with a suicide-related item on the problem list before and after implementation. We assessed clinician knowledge through a survey. All patients with identified suicide risk received treatment to reduce their risk of suicide in the context of usual care.

Results:
For 3,972 new patient visits occurring postimplementation (November 2016–December 2018), the average monthly adherence to the standardized process was 97.7%. The mean monthly incidence of nonspecific active suicidal thoughts was 16%, aborted suicide attempts were 2%, and actual suicide attempts were 3%. The mean monthly incidence of a suicide-related item documented on the problem list was 5.66% in the postimplementation period compared with 1.47% in the 1-year preimplementation. Clinicians demonstrated statistically significant increases in knowledge about suicide risk factors and assessment.

Conclusions:
Standardization of suicide risk assessment processes improved detection and documentation of suicide risk in a pediatric outpatient behavioral health setting.
Clinical Effects of PET-CT Before Definite Concurrent Chemoradiotherapy in Patients With Unresectable Stage IIIA–IIIB Squamous Cell Carcinoma of the Lungs
Authors: Lin Wei-Chun , Chen Wang-Ming , Chen Mingchih , Shia Ben-Chang , Wu Szu-Yuan 
Published: June 2022

Purpose
No large-scale, retrospective cohort study with a long-term follow-up time has yet evaluated the effects of preoperative 18F-FDG PET-CT on survival in patients with unresectable stages IIIA–IIIB squamous cell lung carcinoma (SqCLC) who received definite concurrent chemoradiotherapy (CCRT).

Methods
We included patients with unresectable stages IIIA–IIIB SqCLC receiving definite CCRT and categorized them into 2 groups. The case group comprised patients who underwent pre-CCRT 18F-FDG PET-CT, and the comparison group comprised patients who did not receive pre-CCRT 18F-FDG PET-CT; the groups were matched at a ratio of 1:1.

Results
The matching process yielded a final cohort of 4042 eligible patients (2021 and 2021 in the case and comparison groups, respectively). Multivariable Cox regression analyses revealed a positive correlation between patients with unresectable stages IIIA–IIIB SqCLC receiving definite CCRT and all-cause death in the pre-CCRT 18F-FDG PET-CT (adjusted hazard ratio, 0.85; 95% confidence interval, 0.80–0.91; P < 0.0001).

Conclusion
Pre-CCRT 18F-FDG PET-CT was associated with more favorable survival in both patients with unresectable clinical stage IIIA and those with stage IIIB receiving definite CCRT.
High 11C-Methionine Uptake in a Parasellar Inflammatory Pseudotumor
Authors: Kaida Hayato , Okuda Takeshi , Hanaoka Kohei , Ishii Kazunari 
Published: June 2022

Abstract
A 20-year-old man had left visual impairment and homonymous hemianopsia. MRI findings suggested enlargement of an optic glioma, because optic glioma was indicated by MRI 14 years earlier without a definite pathological diagnosis. 11C-methionine (MET) PET showed high uptake in the tumor in the parasellar region. Transnasal endoscopic biopsy was performed, and an inflammatory pseudotumor (IPT) was diagnosed based on histopathological findings. High MET uptake in a parasellar IPT has apparently not been previously reported. Clinicians should be aware of the possibility of high MET uptake in IPT, because this image could provide an interpretation pitfall.
Comparison of 68Ga-FAPI PET and 18F-FDG PET Findings in a Patient With Bilateral Breast Cancer of 2 Different Histopathologies
Authors: Choudhury Sayak , Shah Sneha , Shet Tanuja , Agrawal Archi , Purandare Nilendu , Puranik Ameya , Rangarajan Venkatesh 
Published: June 2022

Abstract
We present the findings of 18F-FDG PET and 68Ga–fibroblast activation protein PET scans done in a case of bilateral breast carcinoma with 2 different histopathology, left breast tubulolobular carcinoma and right breast invasive breast carcinoma of no special type.
Clozapine Use in a Movement Disorder Clinic
Authors: Friedman Joseph H. , Hershkowitz Dylan 
Published: June 2022

Background
Clozapine is an effective antipsychotic for Parkinson’s disease (PD) that does not worsen motor function and can improve tremor. It is approved for PD psychosis in Europe and Australia.

Objective
The aim of this study was to report on the use of clozapine in a movement disorder clinic.

Methods
We report on patients monitored during the COVID-19 pandemic in clinic over a 7-month period.

Results
Sixty-five patients were seen, of whom 50 had PD. Thirty-one were treated for psychosis, 18 for refractory tremor and 1 for levodopa dyskinesias. The remainder had psychotic symptoms with dementia with Lewy bodies (n = 2) or other movement disorders. Four had clozapine discontinued because of sedation and 1 for agranulocytosis. Three had clozapine temporarily halted because of granulocytopenia but were rechallenged successfully.

Conclusions
When comparing clozapine use in this clinic as compared with others, we deduce that clozapine is likely significantly underutilized in the United States.
Probable Miglustat-Induced Psychosis in a Child With Niemann-Pick Type C
Authors: Koç Yekedüz Merve , Öncül Ümmühan , Köse Engin , Eminoğlu Fatma Tuba 
Published: June 2022

Background
Niemann-Pick disease type C (NP-C) is a neurodegenerative lysosomal disease in which psychiatric symptoms, such as psychosis, can also be observed. Miglustat is indicated in cases with progressive neurological manifestations, and although there have been studies reporting that miglustat completely cures psychosis, it has been recently observed that miglustat may also trigger psychosis. We report on a rare case of probable miglustat-induced psychosis in a patient with NP-C.

Case
A 21-year-old female patient presented with a complaint of social isolation that started at the age of 6 years. During clinical follow-up, the patient's clinical progress deteriorated, and ocular apraxia, ataxia, seizures, and dementia developed at the age of 15 years. A genetic investigation was performed, and a homozygous p.P120S (c.358C > T) variant was detected in the NPC2 gene. Miglustat was initiated at the age of 15 years, and during the 6 months of treatment, psychotic symptoms such as unwarranted anger, suspiciousness, and delusions developed. Consequently, the miglustat was discontinued by the parents of the patient, and the psychosis completely disappeared. The patient has experienced no further psychotic episodes in the approximately 5.5 years following the discontinuation of therapy.

Conclusion
Although a positive effect of miglustat on neurological and psychiatric symptoms has been reported, there exists a risk of psychosis being triggered. To the best of our knowledge, this is the first case of pediatric NP-C to develop psychosis after miglustat to be reported in literature. Further studies of such cases are needed to understand the impact of miglustat on psychiatric symptoms in NP-C.
Interstitial Notch signaling regulates nephron development via the Gata3-Renin axis in the mouse kidney
Authors: Chung Eunah , Adam Mike , Potter Andrew S. , Marshall Sara M. , Potter S. Steven , Park Joo-Seop 
Published: June 2022

ABSTRACTNotch signaling in the renal interstitium is known to be required for the formation of mesangial cells and Ren1 (Renin)-expressing cells. However, little is known about how interstitial Notch signaling affects nephron development. We found that blocking Notch signaling in the renal interstitium in mice caused developmental arrest of proximal tubules accompanied by defective formation of mesangial cells. We examined the interstitial Pdgfrb mutant kidney which exhibits a similar mesangial cell defect and found that the Pdgfrb mutant kidney showed normal proximal tubule development, suggesting that the absence of mesangial cells was not the cause of defective proximal tubule development. Our single cell RNA-seq analysis of the interstitial Rbpj mutant kidney showed that a subset of proximal tubule genes were downregulated in the mutant kidney and that Gata3 was downregulated in the mutant interstitium during the development of Ren1-expressing cells. We found that deleting Gata3 in the interstitium caused the loss of Renin and the developmental arrest of proximal tubules, phenocopying the interstitial Notch/Rbpj mutants. Our results suggest that interstitial Notch signaling regulates the development of proximal tubules via the Gata3-Renin axis in the mouse kidney.
CSF p-tau181 Increases in Patients with Neuronal Intranuclear Inclusion Disease without Amyloid Burden
Authors: Kurihara Masanori , Komatsu Hiroki , Sengoku Renpei , Shibukawa Mari , Morimoto Satoru , Matsubara Tomoyasu , Arakawa Akira , Orita Makoto , Ishibashi Kenji , Mitsutake Akihiko , Shibata Shota , Ishiura Hiroyuki , Adachi Kaori , Ohse Kensuke , Hatano Keiko , Ihara Ryoko , Higashihara Mana , Nishina Yasushi , Tokumaru Aya Midori , Ishii Kenji , Saito Yuko , Murayama Shigeo , Kanemaru Kazutomi , Iwata Atsushi 
Published: June 2022

AbstractBackground and ObjectivesCSF tau phosphorylated at threonine 181 (p-tau181) is a widely used biomarker for Alzheimer’s disease (AD) and has recently been regarded to reflect amyloid-beta (Aβ) and/or p-tau deposition in the AD brain. Although it is important to know how this biomarker reacts in other neurocognitive diseases, CSF p-tau181 in patients with neuronal intranuclear inclusion disease (NIID) has not been studied.MethodsCSF concentrations of p-tau181, total tau, amyloid-beta 1-42 (Aβ42), monoamine metabolites homovanillic acid (HVA), and 5-hydroxyindole acetic acid (5-HIAA) were compared between 12 patients with NIID, 120 patients with symptomatic AD biologically confirmed based on CSF biomarker profiles, and patients clinically diagnosed with other neurocognitive disorders (dementia with Lewy bodies [DLB], 24; frontotemporal dementia [FTD], 13; progressive supranuclear palsy [PSP], 21; and corticobasal syndrome [CBS], 13). Amyloid PET using Pittsburgh compound B (PiB) was performed in six NIID patients.ResultsCSF p-tau181 concentration was significantly higher in NIID (72.7 ± 24.8 pg/mL) compared to DLB, PSP, and CBS and was comparable between NIID and AD. CSF p-tau181 was above the cutoff value (50.0 pg/mL) in 11 of 12 NIID patients (91.7%). Within these patients, only two patients showed decreased CSF Aβ42, and these patients showed negative or mild local accumulation in PiB PET, respectively. PiB PET scans were negative in the remaining 4 patients tested. CSF HVA and 5-HIAA concentrations were significantly higher in patients with NIID compared to disease controls.DiscussionCSF p-tau181 was increased in patients with NIID without amyloid accumulation. Although the deposition of p-tau has not been reported in NIID brains, molecular mechanism of tau phosphorylation or secretion of p-tau may be altered in NIID.
HybAVPnet: a novel hybrid network architecture for antiviral peptides identification
Authors: Ge Ruiquan , Xia Yixiao , Jiang Minchao , Jia Gangyong , Jing Xiaoyang , Li Ye , Cai Yunpeng 
Published: June 2022

AbstractMotivationThe virus poses a great threat to human production and life, thus the research and development of antiviral drugs is urgently needed. Antiviral peptides play an important role in drug design and development. Compared with the time-consuming and laborious wet chemical experiment methods, accurate and rapid identification of antiviral peptides using computational methods is critical. However, it is still challenging to extract effective feature representations from the sequences for the accurate identification of antiviral peptides.ResultsThis study introduces a novel two-step approach, named HybAVPnet, with a hybrid network architecture to identify antiviral peptides based on neural networks and traditional machine learning methods. Firstly, eighteen kinds of features are extracted to predict labels and probabilities by the neural network classifier and LightGBM classifier, respectively. Secondly, the support vector machine classifier is carried out using the predicted probability of the first step to make the final prediction. The experimental result shows HybAVPnet can achieve better and more robust performance compared with the state-of-the-art methods, especially on independent datasets, which makes it useful for the research and development of antiviral drugs. Meanwhile, it can also be extended to other peptide recognition problems because of its generalization ability.Availability and implementationThe predicted model could be downloaded from:https://github.com/greyspring/HybAVPnetContactgespring@hdu.edu.cn;yp.cai@siat.ac.cnSupplementary informationSupplementary data are available atBioinformaticsonline.
Silicon Diode based Flexible and Bioresorbable Optoelectronic Interfaces for Selective Neural Excitation and Inhibition
Authors: Huang Yunxiang , Cui Yuting , Deng Hanjie , Wang Jingjing , Hong Rongqi , Hu Shuhan , Hou Hanqing , Dong Yuanrui , Wang Huachun , Chen Junyu , Li Lizhu , Xie Yang , Sun Pengcheng , Fu Xin , Yin Lan , Xiong Wei , Shi Song-Hai , Luo Minmin , Wang Shirong , Li Xiaojian , Sheng Xing 
Published: June 2022

AbstractThe capability to selectively and precisely modulate neural activities represents a powerful tool for neuroscience research and clinical therapeutics. Traditional electrical stimulations associate with bulky and tethered implants, and optogenetic methods rely on genetic modification for cell targeting. Here, we report an optoelectronic, non-genetic strategy for exciting and inhibiting neural activities, accomplished by bioresorbable, thin-film silicon (Si) diodes. Under illumination, these devices establish polarity-dependent, positive or negative voltages at the semiconductor/solution interface. Such photovoltaic signals enable deterministic depolarization and hyperpolarization of cultured neurons, upregulating and downregulating intracellular calcium dynamics in vitro. Furthermore, flexible, thin-film Si based devices mounted on the nerve tissue selectively activate and silence in vivo activities, both in the peripheral nerve and the brain. Finally, these Si membranes naturally dissolve within the animal body. Such a Si-based material and device platform offers broad potential for biomedical applications.
Gli1+ mesenchymal progenitors contribute to multilineage differentiation in a mouse model of post-traumatic joint injury
Authors: Magallanes Jenny , Liu Nancy Q , Zhang Jiankang , Ouyang Yuxin , Mkaratigwa Tadiwanashe , Bian Fangzhou , Van Handel Ben , Skorka Tautis , Petrigliano Frank A. , Evseenko Denis 
Published: June 2022

AbstractComplex injury and open reconstructive surgeries of the knee often lead to joint dysfunction that may alter the normal biomechanics of the joint. Two major complications that often arise are excessive deposition of fibrotic tissue and acquired heterotopic endochondral ossification. Knee arthrofibrosis is a fibrotic joint disorder where aberrant buildup of scar tissue and adhesions develop around the joint. Heterotopic ossification is ectopic bone formation around the periarticular tissues. Even though arthrofibrosis and heterotopic ossification pose an immense clinical problem, limited studies focus on their cellular and molecular mechanisms. Effective cell-targeted therapeutics are needed, but the cellular origin of both knee disorders remains elusive. Moreover, all the current animal models of knee arthrofibrosis and stiffness are developed in rats and rabbits, limiting genetic experiments that would allow us to explore the contribution of specific cellular targets to these knee pathologies. Here, we present a novel mouse model of post-traumatic joint injury where surgically induced injury and hyperextension of the knee lead to excessive deposition of disorganized collagen in the meniscus, synovium, and joint capsule in addition to formation of extra-skeletal bone in muscle and soft tissues within the joint capsule. As a functional outcome, arthrofibrosis and heterotopic endochondral ossification coupled with a significant increase in total joint stiffness were observed. By employing this model and genetic lineage tracing, we also demonstrate that Gli1+ mesenchymal progenitors proliferate after joint injury and contribute to fibrotic cells in the synovium and ectopic osteoblasts within the joint capsule. These findings demonstrate that Gli1+ cells are a major cellular contributor to knee arthrofibrosis and heterotopic ossification that manifests after knee injury. Our data collectively shows that genetic manipulation of Gli1+ cells in mice may offer a platform for identification of novel therapeutic targets to prevent chronic knee joint dysfunction after chronic injury.
Precision Discovery of Novel Inhibitors of Cancer Target HsMetAP1 from Vast Metagenomic Diversity
Authors: Liu Oliver W. , Akers Scott , Alvarez Gabriella , Brown Stephanie , Cai Wenlong , Charlop-Powers Zachary , Crispell Kevin , Eyles Tom H. , Ganesh Sangita , Goh Ee-Been , Haverty Peter M. , Hwang William W. , Jamison Matthew , Kulp John L. , Kulp John L. , Kurtz Zachary , Lubbe Andrea , Milshteyn Aleksandr , Mokthari Parisa , Naylor Stephen G. , Oteng-Pabi Samuel , Overacker Ross , Robertson Andrew W. , van Aggelen Helen , Viswanathan Usha , Yang Xiao , Yoder Sam , Colletti Steven L. , Scannell Devin R. 
Published: June 2022

ABSTRACTMicrobial natural products have long been a rich source of human therapeutics. While the chemical diversity encoded in the genomes of microbes is large, this modality has waned as fermentation-based discovery methods have suffered from rediscovery, inefficient scaling, and incompatibility with target-based discovery paradigms. Here, we leverage a metagenomic partitioning strategy to sequence soil microbiomes at unprecedented depth and quality. We then couple these data with target-focused, in silico search strategies and synthetic biology to discover multiple novel natural product inhibitors of human methionine aminopeptidase-1 (HsMetAP1), a validated oncology target. For one of these, metapeptin B, we demonstrate sub-micromolar potency, strong selectivity for HsMetAP1 over HsMetAP2 and elucidate structure-activity relationships. Our approach overcomes challenges of traditional natural product methods, accesses vast, untapped chemical diversity in uncultured microbes, and demonstrates computationally-enabled precision mining of modulators of human proteins.
The TRIPLE PHD FINGERS proteins are required for SWI/SNF complex-mediated +1 nucleosome positioning and 5’ transcript length determination in Arabidopsis
Authors: Diego-Martin Borja , Pérez-Alemany Jaime , Candela-Ferre Joan , Corbalán-Acedo Antonio , Pereyra Juan , Alabadí David , Jami-Alahmadi Yasaman , Wohlschlegel James , Gallego-Bartolomé Javier 
Published: June 2022

ABSTRACTEukaryotes have evolved multiple ATP-dependent chromatin remodelers to shape the nucleosome landscape. We recently uncovered an evolutionarily conserved SWItch/Sucrose Non-Fermentable (SWI/SNF) chromatin remodeler complex in plants reminiscent of the mammalian BAF subclass, which specifically incorporates the MINUSCULE (MINU) catalytic subunits and the TRIPLE PHD FINGERS (TPF) signature subunits. Here we report experimental evidence that establishes the functional relevance of TPF proteins for the complex activity. Our results show that depletion of TPF triggers similar pleiotropic phenotypes and molecular defects to those found inminumutants. Moreover, we report the genomic location of MINU2 and TPF proteins as representative members of the plant BAF-like complex and their impact on nucleosome positioning and transcription. These analyses unravel the binding of the complex to thousands of genes where it modulates the position of the +1 nucleosome. These targets tend to produce 5’-shifted transcripts in thetpfandminumutants pointing to the participation of the complex in alternative transcriptional start site usage. Interestingly, there is a remarkable correlation between +1 nucleosome shift and 5’ transcript length change suggesting their functional connection. In summary, this study unravels the function of a plant SWI/SNF complex involved in +1 nucleosome positioning and 5’ transcript length determination.