bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence, associated factors and psychosocial consequences of infertility among women of childbearing age in Guinea: Case of a cross-sectional study with an analytical aim carried out in the five municipalities of Conakry city in 2021
Authors: Leno Niouma Nestor , Kadio Kadio Jean-Jacques Olivier , Camara Alioune , Bley Zézé Charles Romuald , Ehora Djaky Armande Olivia , Aganaye Adoum , Delamou Alexandre 
Published: May 2022

Abstract
Introduction: This study aimed to determine the prevalence of infertility among women of childbearing age, to identify its explanatory factors and to assess its psychosocial consequences among victims. Methods: This was a 6-month cross-sectional study from 18 June to 18 November 2021 involving 4,910 women of childbearing age (15-49 years) in the five municipalities of Conakry. Medians were used to summarise quantitative variables, and proportions were used to summarise categorical variables. Multivariate logistic regression was used to investigate factors associated with infertility (p-value ≤ 0.05). GHQ-28 questionnaire items were used to assess the psychosocial impact of infertility in female victims. Results: The overall prevalence of infertility was 17.8% (5.40% primary and 12.4% secondary infertility). Risk factors associated with infertility were non-education (AOR: 6.146; CI: [4.961 - 7.614]), sexually transmitted infections (AOR: 2.461; CI: [1.997 - 3.033]), (AOR: 3.141; CI: [2.291 - 4.307]), salpingitis (AOR: 4.766; AOR: [3.165 - 7.177]), ovarian cystectomies (AOR: 3.286; CI: [2.008 - 5.376]), uterine myomas (AOR: 3.141; CI: [2.291 - 4.307]) and induced abortions (AOR: 3.372; CI: [2.556 - 4.448]). Social stress, feelings of spousal rejection, anxiety, insomnia, somatic injury, depression and psychosocial dysfunction were the main psychosocial consequences of infertility in the women who experienced it. Conclusion: This study showed the importance of the problem of infertility among women of childbearing age in Conakry. It also helped to understand the need to set up and/or strengthen prevention and care interventions (medical and psychosocial) for victims of infertility. Another study taking into account the clinical and Para clinical explorations of women and men improved the understanding of the phenomenon of infertility in Guinea.
Light and zinc application treatment regulates yield and 2-acetyl-1-pyrroline (2AP) formation in fragrant rice
Authors: shuochen Jiang , lihe Zhang , xiangru Tang , bin Du 
Published: May 2022

Abstract
Background: Improving the yield and aroma content of fragrant rice is the focus of fragrant rice research. Light (L) and Zinc (Zn) management generally cause regulations in the 2-acetyl-1-pyrroline (2AP) accumulation in fragrant rice. In addition, Zn promotes rice growth and improves rice yield, which has the potential to compensate for the negative impact of low light on fragrant rice yield. However, the potential of Zn to improve fragrant rice yield and 2AP content under shading conditions has not been verified.Methods: Field experiments were conducted in the rice season (May-September) in 2019 to 2021. Two light i.e., normal light (NL) and low light (LL) and four Zn levels i.e., 0 kg Zn ha−1 (N0), 1 kg Zn ha−1 (Zn1), 2 kg Zn ha−1(Zn2), and 3 kg Zn ha−1 (Zn3), which applied at booting stage was set up. The grain yield, 2AP contents, Zn content in polish rice, photosynthesis related indicators, MDA content, antioxidant enzyme activity and the biochemical parameters related to 2AP formation were investigated.Results: Shading reduced yield by 8.74% and increased 2AP content by 24.37%. In addition, shading reduced net photosynthetic rate (Pn), superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and increased proline, γ-aminobutyric acid (GABA), and pyrroline-5-carboxylic acid (P5C), proline dehydrogenase (PDH), △1-pyrroline-5-carboxylic acid synthetase (P5CS), malondialdehyde (MDA). With increasing Zn application levels, yield, 2AP, Zn content in polish rice, Pn, proline, P5C, GABA, PDH, P5CS, SOD, CAT and POD increased, and MDA decreased. Significant L and Zn interaction effect on 2AP content was detected, and both shading and increasing Zn application increased the 2AP content.Conclusion: Shading can increase the 2AP content but reduce the yield of fragrant rice. Increasing Zn application under shading conditions can further promote the biosynthesis of 2AP, but the effect of improving yield is limited.
Age and Cervical flexorshelp diagnosedegenerative cervical myelopathy
Authors: Pan Shixin , Liu Chong , Chen Jiarui , Chen Liyi , Liang Tuo , Ye Yongqing , Zhan Xinlin 
Published: May 2022

Abstract
Objective: To evaluate the relationship betweenage and cervical paraspinal muscles (PSM) and degenerative cervical myelopathy (DCM);to establish a diagnostic model for degenerative cervical myelopathy (DCM)with age and Cervical flexors.Methods: A retrospective cohort study of 100 patients with DCM taken at The First Affiliated Hospital of Guangxi Medical University and Wuzhou Red Cross Hospitalbetween December 2018 and June 2020 was performed.C2-C7 Cobb's angle,disc degeneration,endplate degeneration,fatty infiltration and morphology of paraspinal muscles (PSMs)were measured.Receiver operating characteristic (ROC) curves were established to evaluate the value of diagnostic methods. Fisher’s discriminant model was taken to establish a diagnostic model for DCM. Kappa value was calculated to compare different diagnostic models.Results: The multivariate analysis results showed that age and cervical flexors were potential risk factors for DCM.The ability to diagnose DCM alone and in combination using ROC curve method was assessed, showing that the combination of the two indicators had the best diagnostic effect. Fisher's discriminant was used to establish a diagnostic model. Using leave-one-out cross-validation,the diagnostic equation was got:y1=0.33x1+10.302x2-24.139; y2=0.259x1+13.605x2-32.579. Comparison of this diagnostic model and other diagnostic toolsshowed that this diagnostic model performed better (sensitivity 66.7%, specificity 81.3%, accuracy 72.5%), Kappa value 0.363, p=0.011.Conclusion: Age and cervical flexors were potential risk factors for DCM.In this study, a diagnostic model for the diagnosis of DCM was developed using Fisher's discriminant method, combining age and cervical flexors.
The relationship between lung fibrosis, epidermal growth factor receptor and disease outcomes in Covid-19 pneumonia: A postmortem evaluation
Authors: Dülger Seyhan Us , Mutlu Nazmi , Ceylan İlkay , Özhan Erhan 
Published: May 2022

Abstract
The aim of this study is to examine the relationship between the severity of fibrosis in the lung tissue and Epidermal growth factor receptor (EGFR) positivity in patients who died due to Covid-19 pneumonia, demographic characteristics, comorbidities, biochemistry values, and treatments they receive. Fifty patients who died with Covid 19 pneumonia were included in the study. Demographic data of the patients, laboratory tests, thorax computerized tomography findings, comorbidities, length of stay in the intensive care unit (ICU), intubation times and treatments given were noted. Postmortem Tru-cut lung biopsy was performed. EGFR positivity was examined and grouped as negative, mild, moderate and severe. Data analyzed statistically. EGFR involvement were negative in 11 (22%), mild in 20 (40%), moderate in 13 (26%), severe in 6 (12%) patients. The mean C-reactive protein (CRP), d.dimer and mean length of stay in the ICU were found to be statistically different between the groups (respectively p=0.024; p= 0.003; p=0.016). Methylprednisolone usage dose and presence of comorbidity did not differ significantly in EGFR involvement (respectively, p=0.79; p=0.98). CRP and d.dimer values ​​can be a guide for the severity of pulmonary fibrosis that may develop in severe Covid-19 pneumonia patients . The dose of methylprednisolone used does not make a significant difference in the severity of fibrosis. Clinical Trials.gov identifier date and number: 01/13/2022 NCT05290441
Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS
Authors: Kosutova Petra , Mikolka Pavol , Mokra Daniela , Calkovska Andrea 
Published: May 2022

Abstract
Acute respiratory distress syndrome (ARDS) is a common complication of critical illness characterized by lung inflammation, epithelial and endothelial dysfunction, alveolar-capillary leakage, and worsening respiratory failure. The present study aimed to investigate the anti-inflammatory effects of non-selective phosphodiesterase (PDE) inhibitor aminophylline. New Zealand white rabbits were randomly divided into 3 groups: animals with respiratory failure defined as PaO2/FiO2 ratio (P/F) below < 26.7 kPa, and induced by saline lung lavage (ARDS), animals with ARDS treated with intravenous aminophylline (1 mg/kg; ARDS/AMINO), and healthy ventilated controls (Control). All animals were oxygen ventilated for an additional 4 h and respiratory parameters were recorded regularly. Post mortem, the lung tissue was evaluated for oedema formation, markers of inflammation (tumor necrosis factor, TNFα, interleukin (IL)-1β, -6, -8, -10, -13, -18), markers of epithelial damage (receptor for advanced glycation end products, RAGE) and endothelial injury (sphingosine 1-phosphate, S1P), oxidative damage (thiobarbituric acid reactive substances, TBARS, 3-nitrotyrosine, 3NT, total antioxidant capacity, TAC). Aminophylline therapy decreased the levels of pro-inflammatory cytokines, markers of epithelial and endothelial injury, oxidative modifications in lung tissue, reduced lung oedema, and improved lung function parameters compared to untreated ARDS animals. In conclusion, non-selective PDE inhibitor aminophylline showed a significant anti-inflammatory activity suggesting a potential of this drug to be a valuable component of ARDS therapy.
Unique deep-sea bacterial assemblages thriving on different organic matters delivered via in-situ incubators
Authors: Li Jianyang , Dong Chunming , Lai Qiliang , Huang Zhaobin , Zhou Donghui , Gong Linfeng , Wang Guangyi , Shao Zongze 
Published: May 2022

Abstract

Background
The transport of organic matters to the deep sea constantly occurs in global oceans and in accompany with simultaneous microbial remineralization. However, little is known about the impact of fast sinking organic complex on oceanic deep ecosystem and its interactions with microbes throughout the process of sinking and settling on the sea bottom. In this report, to observe the response of indigenous microorganisms to the newly input of organic matters, we developed a series of deep-sea in-situ incubators loaded on the seabed and seamount with various organic substrates of plant and animal origins.
Results
The bacterial diversity at the OTU level varied in accordance to organic substrates and geographic locations, but the functional groups are generally similar. The bacteria of Marinifilaceae were revealed as the key member; in addition, other key decomposers including Spirochaetaceae, Psychromonadaceae, Vibrionaceae, Moritellaceae, and Fusobacteriaceae were also recruited in most assemblages with varied abundance accordingly. Interestingly, nitrogen fixation inside the assemblages occurred in the process of polysaccharide decomposing. The microbial co-occurrence analysis revealed that nutrient availability and energy transfer determine relationships cooperation or competition. Within the assemblages stimulated by substrates short of nitrogen sources, the microbial network was dominated by cooperative relationships, whereas competition relationships overwhelmed the communities thriving on proteinous substrates.
Conclusions
These in-situ observations revealed a unique bacterial assemblage in decomposing diverse newly input organic matters, which may constitute the microbial pumps involved in carbon, sulphur, and nitrogen cycles in oceanic interior.
Media screen time use and mental health in school aged children during the pandemic
Authors: Hmidan Amira , Seguin Diane , Duerden Emma G. 
Published: May 2022

Abstract
Children’s screen time use has increased significantly during the pandemic. Extended school closures and heightened parent stress are associated with children’s behavioural difficulties and time spent watching screens. This cross-sectional longitudinal survey study examined the association between screen time and internalizing and externalizing behaviours in school-aged children (6–12 years) at two time points over the 2020–2021 academic school year. Parents (n = 108) completed survey measures on their parenting styles, stress levels, along with their child’s patterns of screen time and emotional and behavioural difficulties. Children’s average daily screen time was 4.42 hours (SE = 19.04) at baseline and 3.93 hours (SE = 16.35) at 1-year follow up, with no significant change across the school year (p = .12). Increased screen time was associated with a greater incidence of internalizing behaviours in children (p = .03). Children who spent more time on screens and who were in households with parents reporting higher stress levels had increased internalizing behaviours (p = .001). Parent stress was positively associated with children’s externalizing behaviours (p < .001). Children’s screen time use has remained high during the pandemic and is associated with internalizing behaviours. Targeted family intervention plans focused on reducing parent stress and screen time use may aid in improving children’s mental health during the ongoing pandemic.
Performance Evaluation of Viral Transport Medium Produced by BRiCM for Reliable Sample Collection of SARS-CoV-2
Authors: Razu Mamudul Hasan , Monir Bayzid Bin , Moniruzzaman Md. , Sarkar Sawgotom , Akhter Sonia , Kamal Sabiha , Hasan Md. Abu , Afroze Mirola , I Khandaker Md Sharif Uddin , Khan Mala 
Published: May 2022

Abstract
A Viral Transport Medium (VTM) was developed following the Centers for Disease Control and Prevention (CDC), USA, standard operating procedure (SOP) DSR-052-05 with necessary improvisation, was used for storing COVID-19 swab specimens. Considering Bangladesh's present supply chain and storage condition, improvisation was essential for extending sample storage time while retaining efficiency. In-house VTM was produced using Hank's balanced salt solution (HBSS) supplemented with 1% Bovine serum albumin V (BSA), 0.5 µg /ml of Gentamicin Sulfate, and 100 µg/ml of Fluconazole. The produced VTM composition, quality, sterility, specificity, and efficiency were verified in-house and through an independent contract research organization (CRO). An accelerated stability study projected that under the recommended temperature (4°C), it would remain stable for one year and preserve samples for over a month. The Real-Time Reverse Transcriptase-Polymerase Chain Reaction (rRT-PCR) test detected the targeted N gene and ORF1ab gene from VTM stored sample. Our VTM is equally effective as Sansure Biotech VTM in keeping SARS-CoV-2 RNA specimen detectable in rRT-PCR (100% sensitivity and specificity in random and blinded samples). In an import-dependent developing country like Bangladesh, this VTM will ease the fight against pandemics and the supply chain bottleneck of medical and diagnostic equipment caused by it.
Prognostic value of pretreatment 18F-FDG PET/CT metabolic parameters in advanced high- grade serous ovarian cancer
Authors: Morales Daniela Travaglio , Poggio Mónica Coronado , Cabrerizo Carlos Huerga , García Itsaso Losantos , Pozo Cristina Escabias del , Hernandez Carmen Lancha , Marina Sonia Rodado , Gadea Luis Domínguez 
Published: May 2022

Abstract
Background: Patients with advanced high-grade serous ovarian cancer (HGSOC) have a poor prognosis. Hence there have been attempts to find prognostic markers to identify patients with higher risk of relapse. Radiomic features have been proved as an emerging tool in different neoplasms. However, in ovarian cancer very few studies have investigated the role of metabolic parameters at diagnosis. The aim of our work was to assess the prognostic value of pretreatment 18F-FDG PET/CT quantitative metabolic parameters in patients with advanced high-grade serous ovarian cancer. A review of 47 patients diagnosed of advanced high grade serous ovarian cancer between 2016 and 2020 in our center was performed, evaluating pretreatment 18F-FDG-PET/CT metabolic parameters: maximum standardized uptake value (SUVmax), total lesion glycolysis (TLG) and metabolic tumoral volume (MTV). The images were evaluated and quantitative parameters were obtained semiautomatically classifying VOIs as target, non target and total according to SULmax. Clinical data of relapse/progression and final status (exitus/alive) was collected. Disease-free survival (DFS) and overall survival (OS) were calculated. Optimal cutoff values with receiver operating characteristic curve/median values were used. Correlation between metabolic parameters and DFS/OS was made using univariate and survival-curves analysis. Results: Median DFS was 18 months (2.55-55) and OS 33.6 months (2.5-92). MTV total, MTV target, TLG total and TLG target were significantly associated with DFS (p 0.005, 0.011, 0.046 and 0.042 respectively). The survival-curves analysis showed a significant difference of DSF in patients with MTV total >427.8 cm3 and MTV target >434 cm3 versus patients with lower values (18.8 and 15.6 months vs 31 and 30 months, p 0.027 and 0.017 respectively). Patients with higher TLG total and TLG target values tended to have worse DSF, although not statistically significant (p 0.263 and 0.317, respectively). None correlation was found with OS. Conclusion: Pretreatment metabolic parameters MTV total and MTV target have prognostic value in patients with high-grade advanced serous ovarian cancer, being able to predict higher risk of relapse.
Is aggressive care worth it for patients with cancer complicated by pneumonia? Prognostic factors based on pulse oximetry, performance status, and percentage of lymphocytes—a retrospective chart review in a tertiary hospital
Authors: Aramrat Chanchanok , Ratanasiri Thawalrat , Gomutbutra Patama 
Published: May 2022

Abstract
Background: Pneumonia in cancer patients is often problematic in deciding whether to admit and administer antibiotics or pursue a comfort care pathway that may avoid in-hospital death.Aim: to identify factors easily assessed at admission in Thailand’s healthcare context that could serve as prognostic factors for in-hospital death.Design: Using regression analysis to identify the prognostic factors from clinical factors collected at admission. The primary outcome was in-hospital death.Setting/Participants: Data were collected from the electronic medical records of Chiang Mai University Hospital, Thailand, from 2016 to 2017. Data on adult patients with cancer who were admitted due to pneumonia were reviewed.Results: In total, 245 patients were included, and 146 (59.59%) were male. The median age of the patients was 66 years (IQR: 57-75). A total of 72 (29.39%) patients died during admission. From multivariate logistic regression, prognostic factors for in-hospital death included Palliative Performance Scale ≤ 30 (OR: 8.468, 95%CI: 3.471-20.659), Palliative Performance Scale 40-50% (OR: 2.787, 95%CI: 1.337-5.810), percentage of lymphocyte ≤ 8.0% (OR: 2.101, 95%CI: 1.081-4.081), and pulse oximetry ≤ 90% (OR: 2.008, 95%CI: 1.041-3.873).Conclusions: The in-hospital death rate of cancer patients admitted with pneumonia was approximately 30%. PPS 10-30%, PPS 40-50%, percentage of lymphocytes ≤ 8%, and oxygen saturation < 90% could be prognostic factors for in-hospital death. Further prospective studies are needed to investigate the usefulness of these factors.
Evaluation of elemental profile in distant tissues during tumor progression
Authors: Salles Samella , Salles Rebecca , Pavão Mauro Sérgio Gonçalves , Cardoso Simone Coutinho , Stelling Mariana Paranhos 
Published: May 2022

Abstract

Background: Knowing how cancer develops, progresses, and spreads (metastasis) is required to mobilize the development of new ways to prevent, detect, diagnose and treat cancer. Essential elements present functions in tumor progression, promoting proliferation, migration, among other protumoral cellular processes. Understanding how those elements are related to the processes of tumor progression is of great relevance for research. The study of the elemental profile in distant tissues, which can be modulated by tumor cells to promote metastasis, has not been sufficiently investigated. Therefore, the main objective of this study is to evaluate the distribution of medium to heavy elements during tumor progression, focusing on the tumor tissue and distant tissues that could be affected. Results: With this work, it was possible to observe significant changes in the concentrations’ distribution of P, S, K, Ca, Mn, Fe and Cu in distant tissues caused by the presence of tumor cells. It was also possible to detect a greater similarity between tumor tissue (which has the lung as tissue of origin) and a tissue of non-origin, such as the liver, which is an unprecedented result. Moreover, changes in the distributions of concentrations were detected and studied over time for the different tissues analyzed, such as primary tumor, liver and lung, in Control and Tumor groups. Finally, the importance of elements for biological processes of normal cells, as well as tumor cells during tumor progression was also discussed. Conclusions: This work presents as contributions the study of the elemental profile in tumor and distant tissues. Furthermore, this work evaluates several elements of known biological importance (P, S, K, Ca, Mn, Fe, Cu, and Zn) allowing the study of different biological processes involved in cancer.
Paraoxonase 1 is Independently Associated with Pulmonary Pulse Transit Time at Hemodialysis Patients
Authors: Güçlü Aydın , Tur Kağan , Güçlü Kenan 
Published: May 2022

Abstract

Objectives
Pulmonary hypertension (PH) and fibrosis are important complications of hemodialysis patients. There is a direct relationship between oxidative stress and PH. Pulmonary pulse transit time (pPTT) is a valuable parameter for determining PH and fibrosis. Paraoxonases (PON1) are potent antioxidant and anti-inflammatory enzymes. At this study we aimed to determine relationship between PON1 and echocardiography parameters at hemodialysis patients.
Methods
36 patients on maintenance hemodialysis for at least 3 months are enrolled to the study. Blood samples and echocardiography measurements were taken before and after hemodialysis session.
Results
The mean age is 59,3 ± 13,27 years. Mean hemodialysis duration is 6,2 ± 5,5 years. Post hemodialysis PON1 was higher than pre hemodialysis PON1 (r = 0.967, p < 0.0001). Post hemodialysis PON1 and post hemodialysis pPTT were negatively correlated (r=-0.410, p = 0.009). Pre hemodialysis PON1 and pre hemodialysis pPTT measurements were negatively correlated (r=-0.381, p = 0.014). Post hemodialysis PON1 and post hemodialysis E’ were positively correlated (r = 0.345, p = 0.050). Multiple linear regression analysis was run to predict PON1 from pPTT, creatinine, CRP and PTH, the model predicted relation p < 0,05, R = 0,527, pPTT (p < 0,05) added statistically significant to the prediction and CRP (p = 0,055) is close to statistical significance.
Conclusion
Our study showed first time at the literature that there is an independent correlation between pPTT, an indicator for PH and pulmonary fibrosis, and PON1.