bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic Review of Genetic Polymorphisms Associated with Acute Pain and Relevant Acute Toxicities Induced by Radiotherapy for Head and Neck Cancer
Authors: Salama Vivian , Geng Yimin , Rigert Jillian , Fuller Clifton D. , Moreno Amy C. , Shete Sanjay , Reyes-Gibby Cielito C. 
Published: November 2022

AbstractBackground/objectivePain is the most common acute toxicity following radiation therapy (RT) for head and neck cancer (HNC). The multifactorial origin of radiotherapy-induced pain makes it highly challenging to manage in HNC patients. Multiple studies have been conducted to identify different germline genetic variants associated with cancer pain, however few of them focused on RT-induced acute pain. In this systematic review, we summarize potential mechanisms of acute pain after radiotherapy in HNC focusing on oral cavity/oropharyngeal cancer and identify genetic variants associated with radiotherapy-induced acute pain and other relevant acute toxicities.MethodsA comprehensive search of Ovid Medline, EMBASE and Web of Science databases using concepts and terms including “Variants”, “Polymorphisms”, “Radiotherapy”, “Acute pain”, “Acute toxicity” published up to February 28, 2022 was performed by two reviewers. Review articles and citations were reviewed manually. The reported SNPs associated with RT-induced acute pain and toxicities were reported, and the molecular function of the associated genes and pathways were described based on genetic annotation using The Human Gene Database; GeneCards.ResultsA total of 386 articles were identified electronically and an additional 8 articles were included after manual search. 39 articles were finally included. 51 variants were associated with 40 genes, of which 30 % had function in DNA damage response and repair, 25% in inflammatory and immune response, 17.5 % in cell death or cell cycle, and were associated with RT-inflammatory pain and acute mucositis or dermatitis. 4 variants in 4 genes were associated with neuropathy and neuropathic pain. 13 variants in 10 genes and were associated with RT-induced mixed types of post-RT-pain.ConclusionDifferent types of pain develop after RT, including inflammatory pain (acute mucositis and acute skin reaction); neuropathic pain; nociceptive pain; and mixed oral pain. Genetic variants involved in DNA damage response and repair, cell death, inflammation and neuropathic pathways may affect pain presentation post-RT. These variants could be used for acute pain prediction and personalized pain management in HNC patients receiving RT.
Ethical reporting of research on violence against women and children during COVID-19: Analysis of 75 studies and recommendations for future guidelines
Authors: Peterman Amber , Devries Karen , Guedes Alessandra , Chandan Joht Singh , Minhas Sonica , Hui Lim Rachel Qian , Gennari Floriza , Bhatia Amiya 
Published: November 2022

AbstractChanges in research practice during the COVID-19 pandemic necessitates renewed attention to ethical protocols and reporting for data collection on sensitive topics. We systematically searched journal publications from the start of the pandemic to November 2021, identifying 75 studies that collected primary data on violence against women and children. We assess the transparency of ethics reporting and adherence to relevant guidelines against a 14-item checklist of best practices. Studies reported adhering to best practices on 31% of scored items with highest reporting for ethical clearance (87%) and informed consent/assent (84/83%) and lowest reporting for facilitating referrals for minors and soliciting participant feedback (both 0%). Violence studies of primary data collected during COVID-19 report on few ethical standards, obscuring stakeholder ability to enforce a ‘do no harm’ approach and to assess the reliability of findings. We offer recommendations and guidelines to improve future reporting and implementation of ethics within violence studies.
Alpha closed-loop auditory stimulation modulates waking alpha oscillations and sleep onset dynamics in a phase-dependent manner in humans
Authors: Hebron Henry , Lugli Beatrice , Dimitrova Radost , Jaramillo Valeria , Rhodes Edward , Grossman Nir , Dijk Derk-Jan , Violante Ines R. 
Published: November 2022

AbstractAlpha oscillations play a vital role in managing the brain’s resources, inhibiting neural activity as a function of their phase and amplitude, and are changed in many brain disorders. Developing minimally invasive tools to modulate alpha activity and identifying the parameters that determine its response to exogenous modulators, is essential for the implementation of focussed interventions. We introduce Alpha Closed-Loop Auditory Stimulation (αCLAS) as an EEG-based method to augment and investigate these brain rhythms in humans with specificity and selectivity, using targeted auditory stimulation. Across three independent studies, we demonstrate thatαCLASalters alpha power, frequency, and connectivity in a phase, amplitude and topography-dependent manner. Using a single-pulse-αCLASevoked potentials approach we show that the effects of auditory stimuli on alpha oscillations and resulting evoked potentials can be explained within the theoretical framework of oscillator theory and a phase-reset mechanism. Finally, we demonstrate the functional relevance of our approach by showing thatαCLASmodulates sleep onset dynamics in an alpha phase-dependent manner.
A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design
Authors: Rajasekaran Rohith , Chang Chih-Chia , Weix Elliott W. Z. , Galateo Thomas M. , Coyle Scott M. 
Published: November 2022

AbstractCells self-organize molecules in space and time to generate complex behaviors, but we lack synthetic strategies for engineering spatiotemporal signaling. We present a programmable reaction-diffusion platform for designing protein oscillations, patterns, and circuits in mammalian cells using two bacterial proteins, MinD and MinE (MinDE). MinDE circuits act like “single-cell radios”, emitting frequency-barcoded fluorescence signals that can be spectrally isolated and analyzed using digital signal processing tools. We define how to genetically program these signals and modulate their dynamics using engineerable protein-protein interactions. By connecting MinDE to endogenous cellular pathways, we built circuits that broadcast frequency-barcoded single-cell kinase activity or that synthetically pattern actin polymerization. Our work establishes a new paradigm for probing and engineering cellular activities at length and timescales critical for biological function.
Aim18p and Aim46p are CHI-domain-containing mitochondrial hemoproteins inSaccharomyces cerevisiae
Authors: Schmitz Jonathan M. , Wolters John F. , Murray Nathan H. , Guerra Rachel M. , Bingman Craig A. , Hittinger Chris Todd , Pagliarini David J. 
Published: November 2022

ABSTRACTChalcone isomerases (CHIs) have well-established roles in the biosynthesis of plant flavonoid metabolites.Saccharomyces cerevisiaepossesses two predicted CHI-like proteins, Aim18p (encoded by YHR198C) and Aim46p (YHR199C), but it lacks other enzymes of the flavonoid pathway, suggesting that Aim18p and Aim46p employ the CHI fold for distinct purposes. Here, we demonstrate that Aim18p and Aim46p reside on the mitochondrial inner membrane and adopt CHI folds, but they lack select active site residues and possess an extra fungal-specific loop. Consistent with these differences, Aim18p and Aim46p lack chalcone isomerase activity and also the fatty acid-binding capabilities of other CHI-like proteins, but instead bind heme. We further show that diverse fungal homologs also bind heme and that Aim18p and Aim46p possess structural homology to a bacterial hemoprotein. Collectively, our work reveals a distinct function and cellular localization for two CHI-like proteins, introduces a new variation of a hemoprotein fold, and suggests that ancestral CHI-like proteins were hemoproteins.
Exploring the properties of the left angular gyrus using TMS-evoked potentials
Authors: Sulcova Dominika , Salman Yasmine , Ivanoiu Adrian , Mouraux André 
Published: November 2022

AbstractThe angular gyrus (AG) is involved in multiple cognitive processes and its structural alterations are commonly observed in many neuropsychiatric syndromes. Since changes in excitability may precede structural changes and clinical symptoms, there is a need for diagnostic tools assessing the functional state of hub brain regions like the AG. The combination of transcranial magnetic stimulation (TMS) and electroencephalography (EEG) can provide such functional readouts by probing the brain response to direct stimulation.This study aimed to characterize TMS-evoked potentials (TEP) elicited by AG stimulation, determine optimal stimulation parameters, and identify TEP biomarkers of AG function. We recorded AG-TEPs in 19 subjects using four TMS orientations and three intensities and compared TEP spatiotemporal features using topographic dissimilarity and microstate analyses. Additionally, we explored the relationship between AG-TEP topography and TMS-evoked muscular activity.Our results showed topographic variability in AG-TEP components P25, N45, and N75. The P25 topography was sensitive to TMS orientation, while the topography of N45 and N75 was highly dependent on both coil orientation and intensity. Interestingly, we found that TMS-evoked muscular activity was also dependent on both these parameters and strongly related to the occurrence of a specific topographic pattern, which therefore possibly corresponds to the somatosensory brain response to muscle contraction.We conclude that the early AG-TEP component P25 likely reflects neural processes triggered by direct AG activation and could provide an index of local excitability. Later components N45 and N75 must be interpreted with caution as they may primarily reflect peripherally evoked activity.
CRISPR-based tools for genetic manipulation in pathogenicSporothrixspecies
Authors: Hatinguais Remi , Leaves Ian , Brown Gordon D. , Brown Alistair J. P. , Brock Matthias , da Silva Roberta Peres 
Published: November 2022

SummarySporothrix brasiliensisis an emerging fungal pathogen frequently associated with zoonotic transmission of sporotrichosis. Although certain virulence factors have been proposed as potential sporotrichosis determinants, the scarcity of molecular tools for reverse genetics studies onSporothrixhas significantly impeded the dissection of mechanisms underlying the disease. Here, we demonstrate that PEG-mediated protoplast transformation is a powerful method for heterologous expression inS. brasiliensis, S. schenckiiandS. chilensis. Combined with CRISPR/Cas9 gene editing, this transformation protocol allowed the deletion of the putative DHN-melanin synthase genepks1, which is a proposed virulence factor ofSporothrixspecies. To improve in locus integration of deletion constructs, we deleted the KU80homologue that is critical for non-homologous end-joining DNA repair. The use ofS. brasiliensisΔku80strains enhanced homologous-directed repair during transformation resulting in increased targeted gene deletion. In conclusion, our CRISPR/Cas9-based transformation protocol provides an efficient tool for targeted gene manipulation inSporothrixspecies.
It’s not easy seeing green: the veridical perception of small spots
Authors: Vanston John Erik , Boehm Alexandra E. , Tuten William S. , Roorda Austin 
Published: November 2022

AbstractWhen single cones are stimulated with spots of 543-nm light presented against a white background, subjects report percepts that vary between predominately red, white, and green. However, light of the same spectral composition viewed over a large field under normal viewing conditions looks invariably green and highly saturated. It remains unknown what stimulus parameters are most important for governing the color appearance in the transition between these two extreme cases. The current study varied the size, intensity and retinal motion of stimuli presented in an adaptive optics scanning laser ophthalmoscope. Stimuli were either stabilized on target locations or allowed to drift across the retina with the eye’s natural motion. Increasing both stimulus size and intensity led to higher likelihoods that monochromatic spots of light were perceived as green, while only higher intensities led to increases in perceived saturation. The data also show an interaction between size and intensity, suggesting that the balance between magnocellular and parvocellular activation may be critical factors for color perception.Surprisingly, under the range of conditions tested, color appearance did not depend on whether stimuli were stabilized or not. Sequential activation of many cones does not appear to drive hue and saturation perception as effectively as simultaneous activation of many cones.
Minimal models of the inspiratory and sigh breathing rhythms of the preBötzinger complex
Authors: Borrus Daniel S. , Grover Cameron J. , Del Negro Christopher A. , Conradi Smith Gregory D. 
Published: November 2022

AbstractThe preBötzinger complex of the lower brainstem generates two breathing-related rhythms: one for inspiration on a timescale of seconds, and another that produces larger amplitude sighs on the order of minutes. We hypothesize that these two disparate rhythms emerge in tandem wherein recurrent excitation gives rise to the inspiratory rhythm while a calcium oscillator generates sighs; distinct neuronal populations are not required. We present several mathematical models that instantiate our working hypothesis including: (1) an activity (firing rate) model and (2) a minimal spiking network model. Both modeling frameworks corroborate the single-population rhythmogenic hypothesis.
Understanding Lesion Progression in a Chronic Model of Cerebral Cavernous Malformations through Combined MRI and Histology
Authors: Fisher Delaney G. , Sharifi Khadijeh A. , Ulutas E. Zeynep , Kumar Jeyan S. , Kalani M. Yashar S. , Miller G. Wilson , Price Richard J. , Tvrdik Petr 
Published: November 2022

AbstractCerebral cavernous malformations (CCM), also known as cavernous angiomas, are blood vessel abnormalities comprised of clusters of grossly enlarged and hemorrhage-prone capillaries. The prevalence in the general population, including asymptomatic cases, is estimated to be 0.5%. Some patients develop severe symptoms, including seizures and focal neurologic deficits, while others have no symptoms. The causes of this remarkable presentation heterogeneity within a primarily monogenic disease remain poorly understood. To address this problem, we have established a chronic mouse model of CCM, induced by postnatal ablation ofKrit1withPdgfb-CreERT. These mice develop CCM lesions gradually over 4-6 months of age throughout of the brain. We examined lesion progression in these mice with T2-weighted 7T MRI protocols. Precise volumetric analysis of individual lesions revealed non-monotonous behavior, with some lesions temporarily growing smaller. However, the cumulative lesional volume invariably increased over time and accelerated after about 3 months. Next, we established a modified protocol for dynamic contrast enhanced (DCE) MR imaging and produced quantitative maps of gadolinium tracer MultiHance in the lesions, indicating a high degree of heterogeneity in lesional permeability. Multivariate comparisons of MRI properties of the lesions with cellular markers for endothelial cells, astrocytes, and microglia revealed that increased cell density surrounding lesions correlates with stability, while increased vasculature within and surrounding lesions may correlate with instability. Our results lay a foundation for better understanding individual lesion properties and provide a comprehensive pre-clinical platform for testing new drug and gene therapies for controlling CCM.
Viewpoint-Dependence and Scene Context Effects Generalize to Depth Rotated 3D Objects
Authors: Kallmayer Aylin , Võ Melissa L.-H. , Draschkow Dejan 
Published: November 2022

AbstractViewpoint effects on object recognition interact with object-scene consistency effects. While recognition of objects seen from “accidental” viewpoints (e.g., a cup from below) is typically impeded compared to processing of objects seen from canonical viewpoints (e.g., the string-side of a guitar), this effect is reduced by meaningful scene context information. In the present study we investigated if these findings established by using photographic images, generalise to 3D models of objects. Using 3D models further allowed us to probe a broad range of viewpoints and empirically establish accidental and canonical viewpoints. In Experiment 1, we presented 3D models of objects from six different viewpoints (0°, 60°, 120°, 180° 240°, 300°) in colour (1a) and grayscaled (1b) in a sequential matching task. Viewpoint had a significant effect on accuracy and response times. Based on the performance in Experiments 1a and 1b, we determined canonical (0°-rotation) and non-canonical (120°-rotation) viewpoints for the stimuli. In Experiment 2, participants again performed a sequential matching task, however now the objects were paired with scene backgrounds which could be either consistent (e.g., a cup in the kitchen) or inconsistent (e.g., a guitar in the bathroom) to the object. Viewpoint interacted significantly with scene consistency in that object recognition was less affected by viewpoint when consistent scene information was provided, compared to inconsistent information. Our results show that viewpoint-dependence and scene context effects generalize to depth rotated 3D objects. This supports the important role object-scene processing plays for object constancy.
Development of the digital economy, transformation of the economic structure and leaping of the middle-income trap
Authors: Qi Yudong , Chu Xi 
Published: November 2022

PurposeCurrently, China’s economy is in the critical phase of transforming economic development patterns and replacing old growth drivers with new ones. Whether it can successfully overcome the “middle-income trap” has become a significant issue attracting wide attention.Design/methodology/approachDriven by underlying digital technologies such as artificial intelligence, blockchain, cloud computing and big data, the fourth industrial revolution featuring the booming digital economy has provided significant opportunities for China’s economy to “overtake” and overcome the “middle-income trap”. The transformation of economic development pattern, the optimization of industrial structure, and the change of growth drivers, brought by the deep integration of digital and real economies are the keys to leaping over the “middle-income trap”.FindingsFrom the supply side, the digital economy can improve the quality and efficiency of the supply side and promote the supply-side structural reform and economic growth from the following three aspects: First, promote the quality, efficiency and diversification of the supply system; second, promote networking, opening-up and synergy in the innovation system and third, promote the socialization, modularization and flexibility of production pattern. From the demand side, the digital economy can boost the new drivers of the “troika” of economic growth consisting of consumption, exports and investment by changing the market investment direction, promoting consumption upgrade and fostering export strengths. However, once these two attributes interact with each other, especially when data is combined with capital, the most adhesive factor in the market economy, a series of new social relations will then be produced based on the technical attribute, resulting in significant adjustments in social relations, involving both positive and negative externalities.Originality/valueTo overcome the “middle-income trap”, it is necessary to adapt to the laws of economic evolution and promote a fundamental change in economic growth drivers; boost the high-quality development of the digital economy by strengthening the support role of data in the digital economy; and accelerate digital industrialization and industrial digitalization to realize the integration of digital and real economies.
Moving toward a circular economy in manufacturing organizations: the role of circular stakeholder engagement practices
Authors: Fobbe Lea , Hilletofth Per 
Published: November 2022

PurposeThe circular economy (CE) approach has been acknowledged as key for manufacturing organizations wishing to overcome sustainability challenges. However, the transition has been slow. Stakeholder engagement is a driver of the transition, but there is limited knowledge on stakeholder engagement practices in a CE context. The purpose of this paper is thus to explore with whom, on what and how organizations engage with stakeholders to implement CE as part of sustainability efforts.Design/methodology/approachThis study is situated at the intersection of CE, stakeholder theory and supply chain literature. A case study with three Swedish manufacturing organizations was conducted to explore stakeholder engagement practices that facilitate the implementation of CE in organizational practice and the supply chain, considering conceptual differences between stakeholder engagement for sustainability and CE.FindingsThis study provides empirical evidence on how manufacturing organizations engage stakeholders to implement CE as part of organizations' sustainability efforts. The study highlights that manufacturing organizations have to move not only from linear to circular resource flows, but also from linear to circular stakeholder engagement. Such engagement can be achieved by extending with whom, expanding on what and leveling up how stakeholders are engaged.Originality/valueThis study provides an enhanced conceptual understanding of stakeholder engagement in the CE context and discusses differences regarding stakeholder engagement based on linear thinking. The study emphasizes the role of circular stakeholder engagement practices for the transition toward CE in manufacturing organizations.
The role of website features in the branding of higher education institutions
Authors: Ghorbanzadeh Davood 
Published: November 2022

PurposeThis study investigates the effect of university website features and value co-creation behaviors of students on promoting brand image and brand reputation at Islamic Azad University in Iran.Design/methodology/approachThe study is quantitative. Using convenience sampling techniques, a responsive group of 384 students was chosen from the Islamic Azad University of Tehran in Iran. Survey methods were used for data collection. Partial least squares-structural equation modeling was used to test the derived hypotheses.FindingsThe present research findings indicated that website features have a positive effect on fostering value co-creation behaviors (participation and citizenship behavior), and participation behavior, in turn, improves university's brand image and reputation. At the same time, among value co-creation behaviors, citizenship behavior has no impact on the university's brand image. Finally, the brand image formed through website features and participation behavior positively affects brand reputation.Originality/valueThese findings contribute to the marketing literature by empirically validating the three elements in the website features construct, providing intelligence on how website features can drive value co-creation behaviors, brand image and reputation.