bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential predictive and therapeutic applications of small extracellular vesicles-derived circPARD3B in osteoarthritis
Authors: Lin Zhiguo , Ma Yeye , Zhu Xiaoying , Dai Siming , Sun Wentian , Li Wenjing , Niu Sijia , Chu Maolin , Zhang Juan 
Published: October 2022

Background: Heterogeneous phenotypes that display distinct common characteristics of osteoarthritis (OA) are not well defined and will be helpful in identifying more customized therapeutic options for OA. Circular RNAs (circRNAs) have attracted more and more attention due to their role in the progression of OA. Investigating the role of circRNAs in the pathogenesis of OA will contribute to the phenotyping of OA and to individualized treatment.Methods: Small extracellular vesicles (sEV) were isolated from serum samples from patients with OA of different stages and sEV-derived circPARD3B was determined using RT-qPCR analysis. CircPARD3B expression in a stimulated coculture that included OA fibroblast-like synoviocytes (OA-FLS) as well as human dermal microvascular endothelial cells (HDMECs), plus the effects of circPARD3B on the expression of vascular endothelial growth factor (VEGF) long with angiogenic activity, were evaluated in vitro. Based on bioinformatics analysis and luciferase reporter assay (LRA), MiR-326 and sirtuin 1 (SIRT1) were found to be interactive partners of circPARD3B. Mesenchymal stem cells (SMSCs) overexpressing circPARD3B were constructed and SMSCs-derived sEV with overexpressed circPARD3B (OE-circPARD3B-SMSCs-sEV) were obtained to explore the effect of the intervention of circPARD3B combined with SMSCs-sEV-based therapy in vitro and in a OA model induced by collagenase in vivo.Results: Serum sEV-linked circPARD3B was indentified to be significantly decreased in the inflammatory phenotype of OA. Overexpression of circPARD3B was found to inhibit the expression of VEGF, as well as the angiogenesis induced by VEGF in a IL-1β stimulated the co-culture of OA-FLS as well as HDMECs. CircPARD3B is directly bound to miR-326. SIRT1 was considered a novel miR-326 target gene. OE-circPARD3B-SMSCs-sEV significantly reduced VEGF expression in coculture of OA-FLS and HDMECs. Injection of OE-circPARD3B-SMSCs-sEV could also reduce synovial VEGF; additionally, it could further ameliorate OA in the mouse model of OA in vivo.Conclusion: Serum sEV circPARD3B is a potential biomarker that enables the identification of the inflammatory phenotype of patients with OA. Correspondingly, intracellular transfer of circPARD3B through OE-circPARD3B-SMSCs-sEV could postpone disease progression through a functional module regulated angiogenesis of circPARD3B-miR-326-SIRT1, providing a novel therapeutic strategy for OA.
Discovering the potential active ingredients of Qi-Yu-San-Long decoction for anti-oxidation, inhibition of non-small cell lung cancer based on the spectrum-effect relationship combined with chemometric methods
Authors: Huang Mengwen , Li Ruijuan , Yang Mo , Zhou An , Wu Hong , Li Zegeng , Wu Huan 
Published: October 2022

Qi-Yu-San-Long decoction (QYSLD), a traditional Chinese medicine (TCM) prescription, consisting of ten types of herbal medicine which has significant clinical efficacy in the treatment of non-small cell lung cancer (NSCLC). However, the bioactive ingredients of QYSLD remain unclear, due to their “multi-ingredients” and “multi-targets” features. This study aimed to construct a spectrum-effect correlation analysis model and screen the potential active components of QYSLD. A fingerprint method based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) was developed and validated to obtain seventy common peaks of ten batches of QYSLD. The results of methodological evaluation, including precision, repeatability and stability, were less than 8.19%. In terms of linearity, eleven common components did not reach the linear standard (R2 &lt; 0.99), they were removed before spectrum-effect relationship analysis. After treated with ten batches of QYSLD, the results of DPPH and FRAP assays ranged from 1.59 to 5.50 mg mL−1 and 143.83–873.83 μmol L−1, respectively. Meanwhile, the cell viabilities of A549 cells treated with QYSLD samples ranged from 21.73% to 85.71%. The relative healing rates ranged from 21.50% to 44.46%. The number of migrated and invaded cells ranged from 12.00 to 68.67 and 7.67 to 27.00, respectively. Then, the potential active components of QYSLD were screened through spectrum-effect relationship constructed by grey correlation analysis (GRA), partial least squares regression (PLSR) and backpropagation neural network (BP-ANN). The results were as follow: 1) eight ingredients of QYSLD were relevant to DPPH free radical scavenging ability; 2) nine ingredients were relevant to FRAP; 3) six ingredients were relevant to inhibit the proliferation ability of A549 cells; 4) twenty-two ingredients were relevant to inhibit the horizontal migration ability; 5) five ingredients were relevant to inhibit the vertical migration ability; 6) twelve ingredients were relevant to inhibit the invasion ability. Confirmatory experiments showed that compared with the unscreened ingredients, the potential active ingredients screened by the spectrum-effect relationship had better antioxidant and anti-NSCLC effects. In general, this study found the potential active ingredients in QYSLD. Meanwhile, the established method provided a valuable reference model for the potential active ingredients of TCM.
Hesperidin protects rats’ liver and kidney from oxidative damage and physiological disruption induced by nickel oxide nanoparticles
Authors: Abd-Eltawab Tammam Ahmed , A. Khalaf Abdel Azeim , R. Zaki Amr , Mansour Khalifa Mohamed , A. Ibrahim Marwa , M. Mekkawy Aya , E. Abdelrahman Rehab , Farghali Ahmed , A. Noshy Peter 
Published: October 2022

Background: Nickel oxide nanoparticles (NiO-NPs) have recently been utilized in various advanced industrial fields like lithium-ion micro batteries, nanofibers, electrochromic devices, and several biomedical applications. NiO-NPs are classified as extremely toxic substances as they can cause long-term harm to the environment and aquatic life. Moreover, frequent and prolonged exposure can affect human and animal health, causing skin allergies and major toxic consequences, such as hepatorenal toxicity. Hesperidin (HSP) has been proven to possess anti-inflammatory, antioxidant, and free radical scavenging activities.Objective: This study aimed to investigate the underlying protective mechanisms and effects of HSP against NiO-NPs-induced hepatorenal toxicities in rats.Materials and Methods: Forty male Wistar rats were randomly divided into four groups (n = 10 in each). The first group served as a Control group. For 8 weeks, the second group was administered NiO-NPs (100 mg/kg/day), and the third group was given HSP (100 mg/kg/day) via oral gavage for both groups. The fourth group received NiO-NPs and HSP concurrently in the same oral daily doses and duration as the second and third groups.Results: NiO-NPs administration revealed a significant increase in plasma biomarkers of nephrotoxicity (urea, creatinine) and hepatotoxicity (ALT, AST) in NiO-NPs group compared to Control group (p &lt; 0.05). In addition, NiO-NPs administration resulted in a substantial increase in malondialdehyde levels with a significant drop in catalase activity and GSH content in Group II. Also, a significant decreased expression of Nrf-2 and Bcl-2 mRNA levels and upregulation of TNF-α, NF-kβ and BAX in the liver and kidney of NiO-NPs group were also detected. Histologically, the liver and kidney of rats of NiO-NPs group showed significant histopathological disturbances, with a substantial increase in the proliferating cell nuclear antigen (PCNA) positive hepatocytes and renal tubular cells in the NiO-NPs group compared to Control and HSP groups (p &lt; 0.05). In contrast, concomitant administration of HSP with NiO-NPs in group IV showed a significant biochemical, histopathological, and immunohistochemical improvement compared to NiO-NPs group.Conclusion: Co-administration of HSP with NiO-NPs significantly ameliorated most of the NiO-NPs-induced hepatorenal toxicities in male rats.
Facilitators and barriers to health enhancing physical activity in individuals with severe functional limitations after stroke: A qualitative study
Authors: Reicherzer Leah , Wirz Markus , Wieber Frank , Graf Eveline S. 
Published: October 2022

BackgroundPatients with chronic conditions are less physically active than the general population despite knowledge of positive effects on physical and mental health. There is a variety of reasons preventing people with disabilities from achieving levels of physical activities resulting in health benefits. However, less is known about potential facilitators and barriers for physical activity (PA) in people with severe movement impairments. The aim of this study was to identify obstacles and facilitators of PA in individuals with severe disabilities.Materials and methodsUsing a qualitative approach to explore individuals’ subjective perspectives in depth, five community-dwelling adults (age 52–72, 2 female, 3 male) living with chronic mobility impairments after stroke that restrict independent PA were interviewed. A semi structured topic guide based on the theoretical domains framework was utilized. The interview data was analyzed thematically, and the theoretical domains framework constructs were mapped onto the main and sub-categories.ResultsThe six main categories of facilitators and barriers along the capability, opportunity, motivation–behavior (COM-B) framework were: (1) physical capabilities, (2) psychological capabilities, (3) motivation reflective, (4) motivation automatic, (5) opportunity physical, and (6) opportunity social. The physical capabilities to independently perform PA were variable between participants but were not necessarily perceived as a barrier. Participants were highly motivated to maintain and/or increase their abilities to master their everyday lives as independently as possible. It became clear that a lack of physical opportunities, such as having access to adequate training facilities can present a barrier. Social opportunities in the form of social support, social norms, or comparisons with others can act as both facilitators and barriers.ConclusionWhile confirming known barriers and facilitators that impact the ability of individuals with functional limitations to be active, the findings highlight the need and opportunities for comprehensive service models based on interdisciplinary collaborations.
Barriers to family involvement in the care of patients with chronic mental illnesses: A qualitative study
Authors: Dehbozorgi Raziye , Fereidooni-Moghadam Malek , Shahriari Mohsen , Moghimi-Sarani Ebrahim 
Published: October 2022

IntroductionCaregivers are patients' family members or intimate friends who take care of individuals suffering from chronic mental illnesses without being paid. Evidence has supported the role of family-centered collaborative care in the treatment of patients with chronic mental illnesses. It has also been emphasized by national policies. However, carrying out this type of care is accompanied by challenges in Iran. Considering the importance of family participation in taking care of these patients as well as the necessity to determine its effective factors, the present study aimed to assess the barriers to family involvement in the care of patients with chronic mental illnesses.MethodA conventional content analysis was used to conduct this qualitative study. Thirty four health care providers, patients, and caregivers were interviewed unstructured in-depth face-to-face using purposive sampling. Until saturation of data, sampling and data analysis were conducted simultaneously. Graneheim and Lundman's method was used to record, transcribe, and analyze the interviews.ResultThe results showed that there were many barriers to the collaboration of family in the care of patients with chronic mental illnesses. Accordingly, four main categories and twelve subcategories were extracted from the data as follows: “family-related barriers”, “treatment-related factors”, “disease nature threatening care”, and “mental disease-associated stigma in the society”.ConclusionThe findings presented the barriers to family centers' collaborative care in patients with chronic mental illnesses and the necessary components of family involvement in the care to be used by healthcare managers and policymakers. The reported barriers emphasize the need for the development of structured approaches whose implementation is easy for health care providers, does not require a lot of time and resources, and can improve patient and family outcomes.
The roles of behavioral inhibition/activation systems and impulsivity in problematic smartphone use: A network analysis
Authors: Guo Zhihua , He Yang , Yang Tianqi , Ren Lei , Qiu Rui , Zhu Xia , Wu Shengjun 
Published: October 2022

BackgroundBehavioral inhibition/activation systems (BIS/BAS) and impulsivity are associated with problematic smartphone use (PSU). However, no studies to date have explored how the subdomains of BIS/BAS and the dimensions of impulsivity relate to the components of PSU in a joint framework. This study aimed to examine the relationships between the three constructs at a fine-grained level and identify the central nodes and bridge nodes of their relationships using network analysis.MethodsA regularized partial correlation network of PSU, BIS/BAS, and impulsivity communities was estimated to investigate the connections between variables and determine the expected influence and bridge expected influence for each variable based on data from 325 Chinese adults. PSU, BIS/BAS, and impulsivity were assessed using the Smartphone Application-Based Addiction Scale (SABAS), BIS/BAS scales, and Barratt Impulsiveness Scale-Version 11 (BIS-11), respectively.ResultsIn addition to connections within each community, network analysis revealed that there were connections between different communities, especially connections to PSU. I2 “motor impulsivity” was strongly associated with PSU2 “conflict” and PSU6 “relapse”; BASR “BAS-reward responsiveness” was strongly associated with PSU5 “withdrawal.” Nodes BASR “BAS-reward responsiveness” and PSU6 “relapse” were the most central variables, while nodes BASR “BAS-reward responsiveness” and I2 “motor impulsivity” were the strongest bridge variables.ConclusionThe connections between the subdomains of BIS/BAS and the components of PSU and between the dimensions of impulsivity and the components of PSU may be particularly important in the development and maintenance of PSU. The central variables identified here, along with the bridge variables, could be promising and effective targets for the prevention and intervention of PSU.
Psychosocial beliefs of health providers' intention and behavior of offering HIV testing and counseling services: Estimating their relevance for intervention
Authors: Idris Almutaz Mohammed , Crutzen Rik , Van Den Borne Hubertus W. 
Published: October 2022

BackgroundDiagnosis of people with HIV is vital in achieving the 95-95-95 global targets. The proportion of people with HIV who know they have HIV in Sudan is low. Promoting engagement of healthcare providers (HCPs) in offering HIV Testing and Counseling (HTC) services would improve the percentage of diagnosed people with HIV in the country. This study aims to assess the psychosocial beliefs associated with HCPs' intention and behavior to offer HTC services and their relevance for intervention.MethodsThis institutional cross-sectional study was conducted in Kassala State, from July 2019 to February 2020, among 438 healthcare providers from public health facilities. A self-administered questionnaire was used to assess behavior and intention to offer HTC services and related behavioral, normative, and control beliefs. Data were analyzed using R software. Confidence Interval Based Estimation of Relevance (CIBER) was used to estimate the relevance of the beliefs to interventions.ResultsThe CIBER analysis showed that the belief “It causes many worries for patients if I offer or counsel them about HIV test” was negatively associated with HCPs' intention and behavior to offer HTC services and a highly relevant belief for intervention. The belief “My manager thinks I should offer or counsel patients about HIV test” was positively associated with the behavior and intention to offer HTC services and was a relatively highly relevant belief. The control belief “Patients are at low risk of HIV and do not need offering or counseling about HIV test” was negatively associated with HCPs' intention and behavior and was relevant for intervention. The control belief “If I offered or counseled patients about HIV test, I would spend more time with them” was negatively associated with the intention and behavior of HCPs toward HTC services, with high relevance to target with intervention. The belief “My colleagues think I should offer or counsel patients about HIV test” was weakly associated with behavior and intention, and it is a low relevant belief for intervention.ConclusionDifferent psychosocial beliefs among healthcare providers can influence their intention and behavior to offer HTC services to patients. More relevant beliefs are required to be targeted with interventions to promote the intention and behavior of providing HTC services among health care providers.
Survival and risk factors associated with mortality in people living with HIV from 2005 to 2018 in Nanjing, China
Authors: Zhu Zhengping , Xu Yuanyuan , Wu Sushu , Li Xin , Shi Hongjie , Dong Xiaoxiao , Xu Wenjiong 
Published: October 2022

BackgroundAlthough the introduction of antiretroviral therapy (ART) decreased the mortality of people living with Human Immunodeficiency Virus (PLHIV), substantially, hundreds of thousands of people are dying of AIDS each year. The accurate survival patterns and factors related to death among PLHIV were rarely reported. In this study, we evaluated survival status and identified factors associated with death among PLHIV in Nanjing.MethodsWe conducted a retrospective analysis of PLHIV followed-up in Nanjing and registered to the national HIV/AIDS comprehensive management information system from 2005 to 2018. We used the life table to calculate the cumulative survival rates. We applied the Kaplan-Meier to calculate median survival times and employed cox hazard proportional regression to analyze the associated factors related to death.ResultsThe median survival time of PLHIV was 11.8 (95%CI:11.6–11.9) years from 2005 to 2018. Among 4,235 PLHIV included in this study, 7.5% had died of AIDS-related disease and the AIDS-related mortality rate was 2.0/100 PYs. The cumulative proportion surviving at the end of the interval was 95.2% over the 1st year, 94.0% over the 2nd year, 91.8% over the 5th year, and 85.4% over the 10th year, respectively. PLHIV who unaccepted ART showed a greater risk of death compared to those who accepted ART (AHR = 16.2, 95%CI:11.9~22.2). For baseline CD4 count, compared to CD4 &lt; 200 cell/μL, higher CD4 count was demonstrated as a protective factor, with AHR = 0.2 (95%CI: 0.1~0.3) for ≥500 cell/μL, AHR = 0.3 (95%CI:0.2~0.4) for 350~499 cell/μL, AHR = 0.4 (95%CI:0.3~0.6 for 200~349 cell/μL). In addition, we observed a higher death risk in PLHIV who were screened through outpatient (AHR = 1.6, 95%CI: 1.1~2.2) and inpatient (AHR = 1.6, 95%CI: 1.1~2.5) compared to through VCT; the age of diagnosis was ≥50 years old (AHR = 9.5, 95%CI: 3.7~24.1) and 25~49 years old (AHR = 5.0, 95%CI: 2.0~12.3) compared to ≤ 24 years old; educated from junior and below (AHR = 3.4, 95%CI: 2.3~5.1) and Senior high school (AHR = 1.7, 95%CI: 1.1~2.7) compared to college and above.ConclusionThe AIDS-related mortality among PLHIV in Nanjing was relatively low. A higher risk for AIDS-related deaths were observed among PLHIV who unaccepted ART, whose baseline CD4 cell count was&lt;200 cell/μL, older age, and lower educated.
Pollution Characteristics of Water-Soluble Inorganic Ions in PM2.5 from a Mountainous City in Southwest China
Authors: Huang Yimin , Zhang Liuyi , Peng Chao , Chen Yang , Li Tingzhen , Yang Fumo 
Published: October 2022

In order to explore the characteristics of water-soluble inorganic ions (WSIIs) in the atmosphere of Wanzhou, a small mountainous city in Chongqing, four representative seasonal PM2.5 samples and gaseous precursors (SO2 and NO2) were collected from April 2016 to January 2017. The WSIIs (including Cl−, NO3−, SO42−, Na+, NH4 +, K+, Mg2+, and Ca2+) were analyzed by ion chromatography. During the sampling period, daily PM2.5 concentration varied from 3.47 to 156.30 μg·m−3, with an average value of 33.38 μg·m−3, which was lower than the second-level annual limit of NAAQS-China. WSIIs accounted for 55.6% of PM2.5, and 83.1% of them were secondary inorganic ions (SNA, including SO42−, NO3−, and NH4+). The seasonal variations of PM2.5 and WSIIs were similar, with the minimum in summer and the maximum in winter. PM2.5 samples were the most alkaline in summer, weakly alkaline in spring and winter, and close to neutral in fall. The annual average ratio of NO3−/SO42− was 0.54, indicating predominant stationary sources for SNA in Wanzhou. NO3−, SO42−, and NH4+ mainly existed in the form of (NH4)2SO4 and NH4NO3. The results of the principal component analysis (PCA) showed that the major sources of WSIIs in Wanzhou were the mixture of secondary inorganic aerosols, coal combustion, automobile exhaust (49.53%), dust (23.16%), and agriculture activities (9.68%). The results of the backward trajectory analysis showed that aerosol pollution in Wanzhou was mainly caused by local emissions. The enhanced formation of SNA through homogeneous and heterogeneous reactions contributed to the winter PM2.5 pollution event in Wanzhou.
Estabelecendo fluxo de atendimento no ambulatório de infectologia à população privada de liberdade no município de Franco da Rocha–SP
Authors: Castanha Rosemeire 
Published: October 2022

Historicamente, Franco da Rocha é conhecido pelo seu centenário Complexo Hospitalar Psiquiátrico Juquery, uma das mais antigas e maiores colônias psiquiátricas do Brasil, bem como suas unidades prisionais. Com a Política Nacional de Atenção Integral à Saúde das Pessoas Privadas de Liberdade no Sistema Prisional e a incidência de pessoas vivendo com o HIV e outras infecções sexual- mente transmissíveis, ações governamentais foram desenvolvidas visando prioritariamente a atender às peculiaridades dessas pessoas e ao perfil epidemiológico da unidade prisional e do território onde elas se encontram, contemplando ações de prevenção, pro- moção e cuidado em saúde, preconizadas na Política Nacional de Atenção Básica (PNAB), constantes na Relação Nacional de Ações e Serviços de Saúde (RENASES), no âmbito do SUS. Nesse sentido, esse projeto de intervenção teve a intenção de elaborar um plano de ação para organizar o atendimento a essa população no ambulatório de infectologia do município de Franco da Rocha, tendo como objetivo a capacitação dos profissionais no atendimento acolhedor, livre de preconceitos e estigmas, realizando ações educativas que contribuam para entrega do cuidado e, assim, para redução de agravos a saúde dos sentenciados.
Conhecimento clínico e biológico sobre esquistossomose, Doença de Chagas e Leishmaniose em formandos das faculdades de medicina no estado da Bahia
Authors: Espino Bruno Feitosa , De Andrade Gabriela Santos , Silva Guilherme Souza , Da Silva Jonathas Teixeira , Araújo Arthur Oliveira Campos , Carneiro Pedro Paulo de Oliveira 
Published: October 2022

Este trabalho objetiva avaliar o conhecimento dos estudantes de Medicina sobre as Doenças tropicas negligenciadas como: Doença de Chagas, Esquistossomose e Leishmaniose e os fatores a ela relacionados. Trata-se de um estudo descritivo de caráter qualiquantitativo, realizado com acadêmicos do 9º e 12º semestre de Medicina por meio de um questionário semiestruturado associado aos aspectos clínicos e biológicos dessas doenças.