bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nebulized fusion inhibitory peptide protects cynomolgus macaques from measles virus infection
Authors: Reynard Olivier , Gonzalez Claudia , Dumont Claire , Iampietro Mathieu , Ferren Marion , Guellec Sandrine Le , Lajoie Laurie , Mathieu Cyrille , Carpentier Gabrielle , Roseau Georges , Bovier Francesca , Zhu Yun , Pennec Deborah Le , Montharu Jerome , Addetia Amin , Greninger Alexander , Alabi Christopher , Moscona Anne , Vecellio Laurent , Porotto Matteo , Horvat Branka 
Published: June 2022

Abstract
Measles is the most contagious airborne viral infection and the leading cause of child death among vaccine-preventable diseases. We show here that aerosolized lipopeptide fusion inhibitors, derived from heptad-repeat regions of the measles virus (MeV) fusion protein, block respiratory MeV infection in a non-human primate model, the cynomolgus macaque. We used a custom-designed mesh nebulizer to ensure efficient aerosol delivery of peptides to the respiratory tract and demonstrated the absence of adverse effects and lung pathology in macaques. The nebulized peptide efficiently prevented MeV infection, resulting in the complete absence of MeV RNA, MeV-infected cells, and MeV-specific humoral responses in treated animals. This strategy provides an additional shield which complements vaccination to fight against respiratory infection, presenting a proof-of-concept for the aerosol delivery of fusion inhibitory peptides to protect against measles and other airborne viruses, including SARS-CoV-2, in case of high-risk exposure, that can be readily translated to human trials.
FPGA Implementation of DOA Estimation Method Based on Polarization Sensitive Array
Authors: Liu Lutao , Cao Ying , Guo Muran 
Published: June 2022

Abstract
In this paper, the Field Programmable Gate Arrays (FPGAs) implementation of multiple signal classification (MUSIC) algorithm based on polarization sensitive array is discussed for the first time. Directly embedding the conventional complex-valued MUSIC in FPGA will waste a lot of hardware resources and involve high computational burden. Thus, we propose a real-valued direction of arrival (DOA) estimation method based on polarization sensitive arrays for FPGA implementation in this paper. The proposed method introduces a linear transformation on the received signal, thus simplifying the subsequent calculations of the polarization MUSIC algorithm. In addition, the whole FPGA implementation scheme using the proposed real-valued MUSIC based on distributed polarization-sensitive arrays is also developed in this paper. The proposed scheme releases the computational burden via exploiting the parallel calculation of the covariance matrix, the parallel Jacobi algorithm of multiple cleaning of eigenvalue decomposition (EVD), the multi-level spectral peak search method, and the pipelining of each module. The multi-level and multi-group cleaning in the EVD module reuse the resources occupied by a group of cleaning, and the spectral peaks are serially searched in the peak search module. Consequently, compared with the complex-valued MUSIC, the hardware resource consumption and computation burden is dramatically reduced in the proposed scheme.
Prevalence, correlations, and impact of sleep disturbance in Chinese patients with primary pituitary adenomas
Authors: Zhang Xiaomei , Li Yanqing , Gu Yan , Qin Yi , Dong Chen 
Published: June 2022

Abstract
Purpose
Sleep disorders are prevalent among patients with pituitary adenoma (PA) and may lead to disease aggravation and decreased health-related quality of life (HRQoL). Relatively scarce information is available on sleep disturbance in primary PA patients in China. Therefore, this study aims to evaluate the sleeping quality in Chinese primary PA patients and the prevalence, correlates, and impact of sleep disturbance.
Methods
A total of 203 primary PA patients were involved in this cross-sectional study. A series of questionnaires (Pittsburgh Sleep Quality Index (PSQI), MD Anderson Symptom Inventory Brain Tumor for clinical manifestations (MDASI-BT), Hospital Anxiety and Depression Scale (HADS), Multidimensional Fatigue Inventory (MFI-20), and the Short Form 36 health survey (SF-36)) were applied. Independent samples t tests, Mann-Whitney U test, Chi-square analysis, and logistic regression were used to analyze these data.
Results
Our results found that the prevalence of sleep disturbance was 47.8% in primary PA patients. The significant differences were found on the place of residence, alcohol use, depression, anxiety, fatigue, somatic symptom scores and counts, among poor sleepers and good sleepers. Binary logistic regression indicated that fatigue and alcohol use were independently associated with sleep disturbance of primary PA patients. Meanwhile, HRQL of sleep poor patients is clearly lower than those with good sleep.
Conclusions
Primary PA patients in China suffered from sleep disturbance. The results emphasize the importance of fatigue and alcohol use in the sleep disturbance of PA patients. Medical personnel should pay more attention to PA patients with sleep disturbance before the initial diagnosis and preparation for surgery in order to take effective measures early to improve sleep disturbance, with the ultimate goal to improve their QoL.
Coexistence of primary malignancies from liver and ovarian: A rare case report
Authors: Tan Shunde , Chen Jianfei , Fan Jun , Peng Yisheng , Zhu Gang , Li Xinkai , Yang Xiaoli , Li Bo 
Published: June 2022

Abstract
A 54-year-old female presented to the outpatient with a chief complaint of abdominal distention. Abdominal contrast-enhanced CT scan demonstrated mucinous ovarian cancer (MOC) complicating liver metastases. However, the tumor was diagnosed with primary intrahepatic cholangiocarcinoma (PICC)on contrast-enhanced ultrasound (CEUS) and PET-CT. And the two ovarian masses were tended to be mucinous ovarian cystadenomas, while borderline and malignant mucinous tumors could not be excluded completely. Biopsies of the liver was performed due to the patient request. The liver mass was primary intrahepatic cholangiocarcinoma. Her lesions in liver and ovary were resected. The masses of ovarian were mucinous ovarian cystadenoma by intraoperative frozen section analysis. Unfortunately, however, postoperative pathological examinations and immunohistochemistry (IHC) diagnosed as ovarian mucinous cystadenoma malignant change. This was an unusual and rare case of the coexistence of multiple tumors in liver and ovary. The rare pluralism diseases should be noted, when facing complex patients who just a single condition cannot fully reflect clinical findings.
Identification of novel enhancer RNA biomarkers and corresponding prognostic signatures of hepatocellular carcinoma
Authors: Chen Peng , Qi Debin , Sun Keyan , Song Shaohua , Li Tao 
Published: June 2022

Abstract
Background
Researchers now pique an interest in pursuing investigations of enhancer RNAs (eRNAs ). Their roles in the tumorigenesis regulatory mechanism of hepatocellular carcinoma (HCC) remain to be elucidated. Further investigation on eRNAs may shed light on putative prognosis biomarkers and therapeutic targets, which may benefit for early diagnosis of HCC.
Methods
RNA-Seq data and clinical information were obtained from The Cancer Genome Atlas (TCGA). Data were analyzed using R software. Prognosis-related eRNAs were identified, and the target genes of eRNAs were predicted based on the correlation level. We performed enrichment analyses and constructed prognostic model using selected eRNA and target genes, then systematically evaluated prognostic effect. The selected target genes were also analyzed in pan-cancer.
Results
ERNA expression levels were summarized, and 125 prognosis-related eRNAs were identified. Among them, 43 eRNAs were identified because of having putative downstream target genes. After searching literatures, 15 eRNA and target gene pairs were selected for the association with HCC tumorigenesis process or biological behaviors. We chose AL137803.1 for further investigation. The differentially expressed target genes of AL137803.1 in HCC were determined. We performed enrichment analyses and univariate and multivariate cos regression results revealed C5orf58, NDC1, LINC00501 and G6PD could be prognostic signatures of HCC. The prediction value was validated and pan-cancer analyses also demonstrated multiple function of the key eRNA and its target genes.
Conclusion
A novel eRNA AL137803.1 may be a potential biomarker of HCC, besides its putative target genes may serve as prognostic signatures.
Oral Nirmatrelvir and Severe Covid-19 Outcomes During the Omicron Surge
Authors: Arbel Ronen , Sagy Yael Wolff , Hoshen Moshe , Battat Erez , Lavie Gil , Sergienko Ruslan , Friger Michael , Peretz Alon , Yaron Shlomit , Serby Danielle , Hammerman Ariel , Netzer Doron 
Published: June 2022

Abstract
Background
Nirmatrelvir, an inhibitor of the main protease of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has demonstrated a significant decrease in the risk of progression to severe disease in symptomatic high-risk patients infected with the B.1.617.2 (delta) variant of SARS-CoV-2. The effectiveness of nirmatrelvir against the B.1.1.529 variant (omicron) is unknown.
Methods
The study included all Clalit Health Services members, 40 years of age and older, with confirmed infection of SARS-CoV-2 during the omicron surge that were defined as high-risk for severe disease. A Cox proportional-hazards regression model with time-dependent covariates was used to estimate the association between nirmatrelvir treatment and hospitalizations and deaths due to Covid-19, with adjustment for individual sociodemographic factors, coexisting conditions, and prior Covid-19 immunity status.
Results
109,213 participants were eligible for nirmatrelvir therapy during the two-month study period. Among the 42,819 eligible patients aged 65 years and above, 2,504 were treated with nirmatrelvir. Hospitalizations due to Covid-19 occurred in 14 out of the treated and 762 of the untreated patients: adjusted HR 0.33 (95% CI, 0.19 to 0.55). Death due to Covid-19 occurred in 2 treated and 151 untreated patients; adjusted HR: 0.19 (95% CI, 0.05 to 0.76). Among the 66,394 eligible patients 40 to 64 years of age, 1,435 were treated with nirmatrelvir. Hospitalizations due to Covid-19 occurred in 9 treated and 334 untreated patients: adjusted HR 0.78 (95% CI, 0.40 to 1.53). Death due to Covid-19 occurred in 1 treated and 13 untreated patients; adjusted HR: 1.64 (95% CI, 0.40 to 12.95).
Conclusions
Nirmatrelvir therapy was associated with a 67% reduction in Covid-19 hospitalizations and an 81% reduction in Covid-19 mortality in patients 65 years and above. However, no significant benefit in avoidance of severe Covid-19 outcomes was shown in younger adults.
Crystal-storing histiocytosis in the gastric: a case report and review of the literature
Authors: Kong Linghong , Xue Liyan , Zhong Yanfeng , Wang Shenglan , Zheng Danfeng , Wang Lining , Jiao Yang , Zhang Xinpeng , Xue Huizhong , Liu Xiaogang 
Published: June 2022

Abstract
Background: Crystal-storing histiocytosis (CSH) is a rare disorder characterized by the accumulation of nonneoplastic histiocytes containing intracytoplasmic crystallized immunoglobulins. Although CSH can occur in a variety of organs, including the bone marrow, lungs, lymph nodes, liver, spleen, and kidney, gastric CSH is very rare. Case presentation: We present a 69-year-old man with localized gastric CSH, presenting as positive fecal occult blood for 2 days. Gastroscopy showed that there was a piece of irregular whitish focus in the big bend of gastric antrum, which was soft and elastic. Histologically, the biopsied gastric mucosa showed chronic inflammation, mild activity with erosion, and a large number of eosinophilic mononuclear cells containing fibrillary crystalloid inclusion in the lamina propria. Immunohistochemically, these crystal-containing cells were positive for CD68/PGM1 and Igk, which revealed that the cells were histiocytes harboring kappa light chain-restricted immunoglobulin crystals. Electron microscopic examination showed that a large number of high electron density particles were found in the cytoplasm of cells, with the crystal structure, different sizes and shapes.Conclusion: Since this entity is rare and unfamiliar, difficulties in diagnosis may arise. We present a case of localized CSH associated with just such an incidental gastric lesion and reviewed the related literature, in order to improve our understanding of it and avoid misdiagnosis.
Research on marine atmospheric corrosion behavior of AZ31 magnesium alloy based on scientific ocean voyage
Authors: Yang Lihui , Wang Ying , Xu Weichen , Wang Xiutong , Li Yantao , Huang Yanliang , Hou Baorong 
Published: June 2022

Abstract
Corrosion behavior of AZ31 magnesium alloy based on marine scientific ocean voyage was investigated by using scanning electron microscope (SEM), X-Ray Diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and electrochemical measurements. The results revealed that the AZ31 magnesium alloy suffered serious corrosion during exposure in marine atmosphere of ocean voyage. The corrosion rate was 52.23 μm∙y−1 and the maximum depth of corrosion pits was 105.84 μm after exposure for 1 year. The main corrosion products formed on AZ31 magnesium were Mg5(CO3)4(OH)2·4H2O, MgCO3·3H2O and Mg2(OH)3Cl·4H2O. Chloride ion plays an important role in the corrosion process of AZ31 magnesium alloy during ocean voyage. The corrosion of AZ31 magnesium alloy was initiated from pitting corrosion and evolved into general corrosion. This study will provide effective data for the application of magnesium alloy in marine equipment.
Women’s perceptions and experiences of medical treatment for incomplete abortion in Malawi: a mixed methods study
Authors: Chakhame Bertha Magreta , Darj Elisabeth , Mwapasa Mphatso , Kafulafula Ursula Kalimembe , Maluwa Alfred , Odland Jon Øyvind , Odland Maria Lisa 
Published: June 2022

Abstract
Background
Abortion related complications are among the leading causes of maternal mortality in Malawi. Misoprostol is recommended for the treatment of first trimester incomplete abortions but is seldom used for post abortion care in Malawi.
Methods
A descriptive cross-sectional study that used mixed methods was conducted in 3 hospitals in central Malawi. A survey was done on 400 women and in-depth interviews with 24 women receiving misoprostol for incomplete abortion. Convenience and purposive sampling methods were used and data were analysed using STATA 16.0 for quantitative part and thematic analysis for qualitative part.
Results
Data are presented in tables and narratives, three themes emerged around the following areas: experienced effects, support offered, and women’s perceptions. Almost all participants reported having received drugs and counselling as support from health care workers. The majority of participants, 94%, reported satisfaction with the support and described misoprostol as helpful and effective in expelling retained products of conception. Misoprostol was preferred and regarded as the most reliable treatment and beneficial to women by most participants. The majority of the women reported tolerable effects of the drug and 91% would recommend misoprostol to friends.
Conclusions
The use of misoprostol for incomplete abortion in Malawi is acceptable and regarded as helpful and satisfactory among women.
Trial registration:
Not applicable
Microbe Killer Polymeric Films Made by Melt-Compounding and Compression of Peptide Assemblies and Polyethylene
Authors: Kaganovich Michaela , Shlosman Koranit , Goldman Evgeniya , Benchis Marina , Eitan Tamar , Shemesh Rotem , Gamliel Abraham , Reches Meital 
Published: June 2022

Abstract
This paper presents, for the first time, compression molding of peptide assemblies with low-density polyethylene (LDPE) for the robust production of antimicrobial polymeric films. The peptide self-assembles in an aqueous medium into spherical structures with antimicrobial properties. After lyophilization, used to yield a fine powder, these particles were melt-compounded at 160°C with LDPE, followed by compression molding to produce 100 µm thick polymeric films. These films showed a significant reduction in the number of bacteria, viruses, and fungi. These innovative active polymeric films can potentially be applied for medical device wrapping, food packaging, and agriculture applications where crop protection is needed.
Hormesis effect and management of thiophanate-methyl resistance in Lasiodiplodia theobromae isolates
Authors: Silva Erivaldo Laurentino da , Garzón Carla D. , Lopes Ueder Pedro 
Published: June 2022

Abstract
Peduncular rot caused by Lasiodiplodia theobromae is one of the main diseases in papaya crop (Carica papaya). Resistance to the fungicide thiophanate-methyl has been found in isolates of this pathogen in the northeast of Brazil which threatens the productivity of this crop. Therefore, the goals of this study were to evaluate the sensitivity of thiophanate-methyl resistant L. theobromae isolates to other fungicides, and to investigate the hormetic effect of fungicides on populations of the pathogen, aiming at identifying alternatives to manage this important disease. In vitro and in situ assays were conducted, using two fungicides currently registered for management of papaya diseases, as well as some not registered. Demethylation inhibitor fungicides were the most effective to control resistant isolates in vitro. While imazalil, metconazole and tebuconazole were still effective to control papaya peduncular rot, thiabendazole, a fungicide registered to prevent the disease postharvest. This fungicide was ineffective on a resistant isolate and increased its virulence. Additionally, stimulation of mycelial growth in vitro and virulence were observed in the resistant isolate due to exposure to subinhibitory doses of thiophanate-methyl. These observations suggest the need for new strategies to manage the disease in areas with a history of resistance. The fungicide imazalil, registered for postharvest of papaya, is currently a good option to control L. theobromae isolates. Metconazole and tebuconazole, although not registered for this crop, have potential to manage the disease in areas with a history of resistance to thiophanate-methyl.
Injectable oxygen liberating hydrogels increase survival and innervation of neural stem cell grafts prior to vascularization
Authors: Wang Yi , Thomas Jacob , Hong Nansook , Tan Li Lynn , McGillivray Duncan , Perriman Adam , Moriarty Niamh , Parish Clare L , Williams Richard , Jackson Colin , Nisbet David 
Published: June 2022

Abstract
Injectable hydrogels have great potential for use in regenerative medicine as cellular delivery vectors. However, as with other cell-laden transplantable materials, they suffer from issues relating to hypoxia, including poor cell survival, differentiation, and functional integration owing to the lack of an established vascular network. Here, we have engineered a hybrid myoglobin:peptide hydrogel that can concomitantly deliver stem cells and oxygen to the brain to support engraftment until vascularisation can occur naturally. This hybrid hydrogel can modulate cell fate specification within progenitor cell grafts, resulting in a significant increase in neuronal differentiation. The addition of myoglobin to the hydrogel resulted in more extensive innervation within the host tissue from the grafted cells, which is essential for neuronal replacement strategies to ensure functional synaptic connectivity. Further development of this approach could result in greater functional integration of stem cell-derived grafts for the treatment of neural injuries and diseases affecting the central and peripheral nervous systems.
Groundwater quality assessment of north of Iran (Golestan Province) using multivariate factor analysis and GIS techniques
Authors: Nassiri Mohammad , Mehdinejad Mohammad Hadi 
Published: June 2022

AbstractGroundwater is one of the most important sources of drinking water. Since the quality of water directly influences consumers' health, it is essential to assess the quality of water and related factors. This descriptive-analytical study was performed on physical and chemical variables of water samples collected in the dry and wet seasons during 2006–2020 period. Changes in groundwater quality were investigated by factor analysis (FA) model and geographic information system (GIS) software. Based on factor analysis model, EC, TDS, TH and Mg+2 levels were the most important factors affecting the quality of water. Due to the high level of hardness (dry seasons; 389.4 mg/l and wet seasons; 418.4 mg/l) and sodium in water (dry seasons; 138 mg/l and wet seasons; 158 mg/l), necessary measures should be taken before drinking and agriculture uses. Changes in the water quality were more related to natural factors and human activities. The invasion of saline water into the groundwater aquifers in the study area should be considered.
Degradation of the nonylphenol aqueous solution by strong ionization discharge: evaluation of degradation mechanism and the water toxicity of zebrafish
Authors: Yi Chengwu , Yang Liu , Yi Rongjie , Yu Haijun , Zhang Jianan , Nawaz Muhammad Imran 
Published: June 2022

Abstract
Nonylphenol (NP) is a typical environmental endogenous disrupter with low concentration and high toxicity. This paper describes the mechanism of NP degradation in solution by strong ionization dielectric barrier discharge (SIDBD). Furthermore, the degradation performance of NP by SIDBD was tested by changing the equipment voltage, the initial concentration of NP in aqueous solution, pH, and inorganic ions. Degradation pathways of NP were detected using a high-performance liquid chromatography-mass spectrometer. The biological effects of NP degradation were assessed by detecting indicators of embryonic development in zebrafish (survival rate, fetal movement, heartbeat, the body length, behavior, deformity) and adult fish (sex differentiation, weight, ovarian testes pathological section analysis). The results showed when the input O2 was 5 L/min and the voltage was 3.2 kV, the degradation efficiency of NP can reach 99.0% after 60 min of experiment. Equipment voltage, initial concentration of NP in solution, pH, inorganic ions and other factors can influence the degradation efficiency of NP by DBD. At the higher concentration of NP, the greater influence on embryonic development in zebrafish was noticed. Although the effects of NP on zebrafish sex differentiation were not obvious, it showed significant male weight inhibition and decrease in sperm number.
Removal of 17α-ethynylestradiol and β-estradiol using bench-scale constructed wetlands
Authors: Bliss S. N. , Berger K. , Thieme J. , Maldonado Cobos M. , Nyman M. 
Published: June 2022

Abstract
Aquatic ecosystems have been devastated by the continued persistence of the synthetic estrogen compounds β-estradiol and 17α-ethynylestradiol. Common wastewater treatment methods do not reduce these compounds in effluent below problematic concentrations. An emerging cost-effective solution to this problem is the use of constructed wetlands to remove these estrogen compounds. This study analyzed the ability of duckweed (Lemna minor), water hyacinth (Eichhornia crassipes), and water cabbage (Pistia stratiotes) to remove β-estradiol and 17α-ethynylestradiol through the use of bench-scale constructed wetlands over a 15-week period. Estrogen concentration in water was collected over time along with plant nutrient content, contaminant extractions, and media extractions. Results indicated that estrogen concentration was reduced by the plants and soil media. Duckweed was the most effective at 96% removal, followed by water hyacinth at 72% removal, then water cabbage at 35% removal, and lastly sediment media at 9% removal. This study provides evidence for the ability of constructed wetlands to be used as a means to remove estrogen compounds from wastewater and demonstrates differences in plants removal efficiencies, with duckweed being the most effective of the selected plants.