bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of lipoprotein(a) in aortic dissection patients and acute myocardial infarction patients with chest pain: A Cross-sectional Study
Authors: Dong Qinhui , Zhang Yunyan , Zhou Kai , Zhang Hongliang 
Published: August 2022

Abstract
Background: Aortic dissection patients entering the hospital may suffer from chest pain and have a deviation value of lipoprotein(a) (Lp(a)). In the present study, we aimed to investigate the clinical importance of Lp(a) level among aortic dissection(AD) patients, healthy control , and acute myocardial infarction(AMI) patients.Method: Acute AD patients (n=257), AMI patients (n=99) and healthy subjects (n=99) were enrolled into our research. We measured Lp(a) level at the admission. We recorded the baseline demographic data and laboratory outcomes. The endpoint was death situation in patients during one month. Then we performed statistical analysis to determine the association between lp(a) level and diseases. Student’s t-test analysis and multiple logistic regression were used to identify lp(a) level difference among groups Results: Researchers interviewed a total of 257 patients with AAD. AAD patients had higher Lp(a) level than healthy control(165(91,301.5) vs.136(68,222).p＜0.001) and lower Lp(a) level than AMI patients(165(91,301.5) vs.231(123,386).p＜0.001). In one month follow-up, the survivors have a higher Lp(a) level than the death toll. Univariate regression analysis showed that Lp(a) was a significant indicator of aortic dissection(OR 1.001, 95% Cl 1.000-1.003, p=0.039). However, after adjusted for other risk factors, an increased risk of having AAD is not observed for a high level of Lp(a)(OR,1.001;95% Cl 0.999-1.003, p=0.4).Conclusions: Serum lipoprotein(a) levels were significantly higher in AAD patients than healthy control. Moreover, the high lp(a) level was found to be independently associated with a better prognosis of AAD. Higher lipoprotein(a) may enhance vessel atherosclerosis, accelerate thrombosis process and promote inflammation response by avoiding the formation of aortic dissection.
Cardiac TNF Receptor-Associated Factor 7 Mediates the Ubiquitination of Apoptosis Signal-Regulating Kinase 1 and Aggravates Cardiac hypertrophy
Authors: Che Yan , Xia hongxia , Wang Zhao-Peng , Feng Yizhou , Liu Yuting , Yuan Yuan , Zhou Heng , Wang Sha-Sha , Tang Qizhu 
Published: August 2022

Abstract
Tumour necrosis receptor-associated factor 7 (TRAF7) belongs to the tumour necrosis factor receptor (TNFR)-associated factor family and plays an important role in biological processes. Previous studies have shown that TRAF7 mutations lead to congenital defects and malformations of the heart. However, the molecular mechanisms of TRAF7 in the underlying pathogenesis of pathological cardiac hypertrophy remain unknown. Here, we found that the expression of TRAF7 increased gradually during the development of hypertrophy. Accordingly, TRAF7 significantly exacerbated the phenylephrine (PE)-induced enlargement of primary neonatal Sprague Dawley rat cardiomyocytes (NRCMs), whereas TRAF7 knockdown alleviated the hypertrophic phenotype in primary cardiomyocytes. Cardiac-specific overexpression of TRAF7 accelerated hypertrophic phenotype in mice and cardiac-specific TRAF7 conditional knockout mice improved hypertrophic phenotype induced by transverse aortic constriction (TAC). Mechanistically, TRAF7 directly interacted with apoptosis signal-regulating kinase-1 (ASK1) and promoted ASK1 phosphorylation by mediating the K63-linked ubiquitination of ASK1 in response to PE stimulation, which then promoted ASK1 activation and downstream signalling during cardiac hypertrophy. Notably, the pro-hypertrophic effect of TRAF7 was largely blocked by the ASK1 inhibitor, GS4997. In summary, we identified TRAF7 as an essential regulator during cardiac hypertrophy, and modulation of the regulatory axis between TRAF7 and ASK1 could be a novel therapeutic strategy to prevent this pathological process.
The effect of haploidentical hematopoietic stem cell transplantation on comutations based on next-generation sequencing in adult acute myeloid leukemia patients with the FLT3-ITD mutation
Authors: Huang Xiao-Jun , Tang Fei-Fei , Zhao Xiao-Su , Ruan Guo-Rui , Jiang Qian , Jiang Hao , Xu Lan-Ping , Zhang Xiao-hui 
Published: August 2022

Abstract
The objective of this study was to investigate which comutations based on next-generation sequencing (NGS) at diagnosis affect the clinical prognosis of de novo AML patients with FLT3-ITD mutations and the effect of haploidentical hematopoietic stem cell transplantation (haplo-HSCT) on the comutations. We analyzed 95 de novo AML patients with FLT3-ITD mutations from January 2018 to August 2021 based on the NGS 99-gene platform. Forty-one other types of molecular mutations were detected. The most common cooccurring mutations were NPM1 (n = 43, 45.3%) and DNMT3A (n = 21, 22.1%). NPM1 mutation status did not affect the clinical outcomes. AML patients with FLT3-ITD and DNMT3A comutations had significantly worse 3-year DFS (25.2% and 62.6%, P = 0.003) and OS rates (57.3% vs. 73.1%, P = 0.047) than those without DNMT3A mutations and the survival was significantly more favorable after haplo-HSCT than chemotherapy (3-year DFS,77.1% vs. 15.4%, P = 0.009; 3-year OS, 82.8% vs. 46%, P = 0.001, respectively). By multivariate analysis, DNMT3A mutation was a risk factor for DFS and OS, while haplo-HSCT was a protective factor. DNMT3A mutation might be a poor prognostic factor in adult AML patients with FLT3-ITD mutations and haplo-HSCT could overcome the poor prognostic of DNMT3A comutation.
Crude F4 fimbriae extracts: its feasibility to be a candidate for Escherichia coli vaccine development for the prevention of diarrhea disease caused by E. coli in piglets
Authors: Nguyet Luong Thi Yen , Ounjai Puey , Kaeoket Kampon , Ngamwongsatit Natharin 
Published: August 2022

Abstract
Background: Enterotoxigenic Escherichia coli (ETEC) is one of the most important pathogens causing neonatal and post-weaning diarrhea (PWD) in piglets. The F4+ETEC is particularly problematic in pig farms. Here, we present a simple extraction for producing crude F4 fimbriae to be exploited as an antigenic determinant for effective vaccination. Results: The SDS-PAGE and LC-MS/MS analysis revealed that the crude F4 fimbriae extract contains a major band of molecular weight around 24 kDa, corresponding to the size of the major subunit (FaeG) of F4 fimbriae together with several minor bands of different molecular weights. Crude F4 fimbriae extracts (150 µg/dose) were used to immunize 3 four-weeks old piglets. The antiserum was collected and evaluated the level of F4-specific antibodies by ELISA. It was clear that the level of serum pig IgG and pig IgA antibodies against F4 were significantly higher than that against other E. coli strains (F18+ETEC, F41+ETEC and F4-F18-F41-EC) and unvaccinated control (p < 0.01). Moreover, the pig IgG antibody level against other E. coli strains was also detected at considerably higher level than that of the unvaccinated control (p < 0.05). Conclusions: Our results therefore demonstrated that the crude F4 fimbriae obtained from simple extraction not only induce specific immunity against F4+ E. coli but also stimulate cross-immunity against other E. coli strains. The result suggested that crude F4 fimbriae extract from our method can be used for future vaccine development against porcine diarrheal diseases caused by E. coli.
Biomechanical Analysis of Ovariectomized Osteoporosis Rats with Kidney Deficiency
Authors: Lin Chubin , He Xingpeng , Qiu Yuhui , Cui Shaolong , Chang Yu , Babichuk Ivan S. , Ye Tao , Li Pengfei , Yang Jian 
Published: August 2022

Abstract
Background: Osteoporosis patients with chronic kidney disease usually have a higher risk of bone fracture. In traditional Chinese medicine, Kidney-Yang Deficiency and Kidney-Yin Deficiency are two common syndromes for chronic kidney disease patients. You-gui pills are used as a classic prescription to treat Kidney-Yang Deficiency syndrome due to their efficiency. This study evaluated the effect of you-gui pills on biomechanical properties of femurs in Kidney-Yin and Kidney-Yang Deficiency rats.Methods: The ovariectomized female specific pathogen-free rats were divided into four groups, including Kidney-Yin Deficiency and Kidney-Yang Deficiency with and without you-gui pills. All the ovariectomized rats were sacrificed before the femurs on both sides were separated. Micro-CT analyzed the left femur, the right femur was used to perform a three-point bending test for a femoral shaft, compression and surface indentation tests for the femoral head. The obtained mechanical parameters were statistically analyzed by T-test.Results: The effects of you-gui pills on the femur of Kidney-Yin and Kidney-Yang Deficiency rats were different. You-gui pills significantly affect the bone microstructure and biomechanical properties of the femur of Kidney-Yang Deficiency rats. You-gui pills could significantly enhance bone/tissue volume, trabecular thickness and toughness of the femur in Kidney-Yang Deficiency rats.Conclusion: You-gui pills can enhance the anti-fracture ability of the femoral shaft and the anti-deforming capability of the femoral head in Kidney-Yang Deficiency rats, but have no noticeable effect on the femur of Kidney-Yin Deficiency rats. This study reports on the clinical efficacy of you-gui pills in the treatment of bone. Moreover, it presents a comprehensive narrative of the rationale of this method, highlighting its potential for treatment kidney failure, which affects bone strength.
Local buckling behavior of buried pipeline under seismic oblique-reverse fault displacement
Authors: Xu Lingyue , Cheng Xudong , Huang Runkang , Chen Wendi , Hu Wenjun 
Published: August 2022

Abstract

Seismic fault displacement is the main factor leading to the local buckling failure of the buried pipeline, especially crossing the oblique-reverse fault. The 3-D displacement of the oblique-reverse fault makes the local buckling behavior of the buried pipeline more complex. It may be inaccurate to evaluate the local buckling location and degree using the pipe local buckling results under the single fault displacement, affecting the monitoring and safety assessment of the pipeline. In this paper, to determine the pipe local buckling behavior (potential local buckling locations, developing process) under the oblique-reverse fault displacement, a shell and solid element nonlinear contact coupling model of the pipeline crossing oblique-reverse fault is established using ABAQUS. The results reveal two potential local buckling areas and three stages of the local buckling developing process under the oblique-reverse fault displacement. Subsequently, the potential local buckling locations and three stages in the local buckling under different operating conditions is obtained. It proves that using the results of pipe local buckling under single fault to evaluate the pipeline local buckling under composed fault is not safe enough. Consequently, local buckling behavior of the pipeline crossing oblique-reverse fault provides a reference for the preliminary pipe design, detection, and evaluation.
Dapagliflozin improves the dysfunction of HUVECs by downregulating high glucose/high fat-induced autophagy through inhibiting SGLT-2
Authors: Zhong Siyu , Xia Jie , Jiang Tao , Jiang Aihua , Huang Zhimei , Wang Jianping 
Published: August 2022

Abstract
Objective: To investigate the effect of Dapagliflozin (Da) on the disorders of human umbilical vein endothelial cells (HUVECs) induced by high glucose and high fat (HG/HF). Methods: Immunohistochemistry and immunofluorescence were used to detect the SGLT-2 expression in thoracic aortic tissues. After transfected with overexpressed plasmid SLC5A2, autophagy and cell functions of HUVECs were detected with the treatment of autophagy inhibitor 3-MA (5 mM). HUVECs were exposed to mannitol (MAN), glucose/palmitate (Hg/PA), and Hg/PA/Da for 24 h, and the proliferation of HUVECs was detected by CCK-8. The protein expression levels, endothelial cell functions (cell proliferation, migration, tubular formation, apoptosis, and autophagy) in endothelial cells were evaluated. Results: The SGLT-2 expression was found in atherosclerotic human thoracic aorta tissues and HG/PA induced HUVECs (P<0.05). After the overexpression of SGLT-2 in HUVECs, the proliferation, migration and tubule formation ability of HUVECs were inhibited, and autophagy and apoptosis were increased, which were reversed by 3-MA (P<0.05). After the addition of Sodium-glucose co-transporters 2 inhibitor, Dapagliflozin, the proliferation of HUVECs, the tubule formation, autophagy, apoptosis and migration ability of cells inhibited by HG/PA were significantly improved (P<0.05). Moreover, the increased protein expression levels of autophagy and apoptosis in HG/PA induced HUVECs were also decreased by the treatment of Dapagliflozin (P<0.05).Conclusions: Dapagliflozin can improve the dysfunction of high glucose/high fat-induced human umbilical vein endothelial cells by downregulate autophagy through inhibiting SGLT-2.
Transfer of literature data to virtual patient cases: Development and implementation of a method to generate categorical data using Bernoulli-distributed random values
Authors: Schmidt Christian , Kesztyüs Dorothea , Haag Martin , Wilhelm Manfred , Kesztyüs Tibor 
Published: August 2022

Abstract

Background: Teaching medicine is a complex task because medical teachers are also heavily involved in clinical practice. Automatic creation of virtual patient cases would be a great benefit to them, saving them time and providing a wider choice of virtual patient cases for student training. Our aim was to develop and test a computer program which simulates clinical case scenarios based on information about the occurrence of specific symptoms in certain diagnoses from the literature.Methods: Medical literature was searched for suitable diagnoses with information on the respective probabilities of specific symptoms. We developed a computer program that generates virtual patient cases with symptons whose occurrence is randomly generated by Bernoulli experiments, according to probabilities reported in the literature. The number of runs and thus the number of patient cases generated is arbitrary.Results: We illustrate the function of our generator with the exemplary diagnosis “brain abscess” with the related symptoms and the respective probabilities from the literature. With a growing number of repetitions of the Bernoulli experiment, the relative frequencies of occurence increasingly converge with the probabilities from the literature. E.g., the relative frequency for headache after 10.000 repetitions was 0.7267 and, after rounding, equals the mean value of the probability range of 0.73 reported in the literature. The same applies to the other symptoms. Conclusion: The results suggest that an automated creation of virtual patient cases is possible, but with regard to the limitation to symptom constellations, it is not yet suitable for professional use. Based on additional information provided in the literatur, an extension of the generator can be implemented in further research.
Money or ingots? Metrological research on pre-contact Ecuadorian ‘axe-monies’
Authors: Puente Carlos E. Montalvo , Lago Giancarlo , Cardarelli Lorenzo , Molina Juan C. Pérez 
Published: August 2022

Abstract

The Axe-monies, trapezoidal sheet objects made of arsenical copper, are associated with graves and hoards of the Manteño-Huancavilca (600–1532 A.D) and Milagro-Quevedo (400–1532 A.D.) cultures, both located in the coast of Ecuador. Historically, scholars identified these artifacts as monies, relating them to a currency system. This paper aims to test this hypothesis by searching for standardization analyzing an important sample of these objects. Metrological analyses on dimensions (length, width) and weight show that the currency hypothesis is flawed because neither apparent clustering of dimensions nor a tendency to a single (range of) weight value(s) is found. We suggest ‘axe-monies’ were used as ‘ingot-objects’ and copper was a commodity in the framework of exchanges involving different cultures throughout America's Pacific coast.
The Clinical Significance of Radiological Changes Associated with Gliadel® Implantation in Patients with Recurrent Glioblastoma
Authors: Haim Oz , Agur Ariel , Efrat Or-Tal , Valdes Pablo , Ram Zvi , Grossman Rachel 
Published: August 2022

Abstract
Objective: Gliadel® occasionally induces edema following its implantation. We aimed to correlate such post-surgical radiological changes to its efficacy and subsequent survival.Methods: Fifty-six patients with recurrent glioblastoma were treated between 2005-2016 with Gliadel® implantation. Volumetric measurements of MRI features, including FLAIR abnormalities, tumor bulk (volume of gadolinium enhancement on T1) and resection cavity volumes over time were conducted. To assess dynamics over time, linear regression trendlines for each of these were calculated and examined to correlate with survival.Results: Median follow-up after resection was 21.5 months. Median survival post-Gliadel® implantation and overall survival since diagnosis were 12 months and 22 months, respectively. A subgroup of patients (n=6) with a transient increase in FLAIR changes volume over time survived significantly longer post-Gliadel® compared to those who did not demonstrate such change (36 vs 12 months, p=.03). Positive trends, representing overall growth in volume over time, of tumor bulk and resection cavity predicted survival in multivariate analyses (hazard ratios 7.9 and 84, p=.003 and .002, respectively).Conclusions: Increase in tumor bulk and resection cavity over time were associated with decreased survival, while transient FLAIR increase was a favorable prognostic factor. This may represent a transient inflammatory process in the tumor, possibly stemming from a presumed immune-mediated anti-tumor response.
Isolation of lectin from Musa acuminata for evaluation of antibacterial activity against Methicillin-Resistant Staphylococcus aureus and the interaction of banana proteins with certain Enterococcus species
Authors: Ahmed Summra , Baloch Muneera Naz , Moin Syed Faraz , Bilal Hafiz Muhammad , Musa Hina 
Published: August 2022

Abstract
Methicillin Resistant Staphylococcus aureus is an emerging pathogen posing a considerable burden on the healthcare system due to its involvement in skin and soft tissue infections (SSTIs). Lectins are carbohydrate binding proteins found ubiquitously in animals, plants and microorganisms. Extraction and isolation of proteins from Musa acuminata (banana) were performed by using Affinity chromatography with Sephadex G 75 to determine antibiofilm activity of banana proteins against Methicillin Resistant Staphylococcus aureus (MRSA). Enterococcus strains obtained from dairy products, beans and vegetables were also screened for its potential to inhibit growth and subsequently biofilm formation of MRSA by using 96 well microtitre plates. Synergistic effect of cell free supernatant of Enterococcus with proteins from ripe banana were also tested. BanLec was successfully isolated and appeared as 15 KDa band after SDS-PAGE (15%) while multiple bands of unbound protein fractions were observed. The unbound fractions showed inhibition of pathogenic growth and biofilm but BanLec exhibited no significant effect. All the identified Enterococcus strains showed inhibition of biofilm as well as of planktonic cells. The CFS of Enterococcus faecium (LCM002), Enterococcus lactis (LCM003) and Enterococcus durans (LCM004 and LCM005) displayed antagonistic effects against pathogen. The synergistic effect of CFS from E. lactis (LCM003) and unbound proteins showed inhibition of biofilm and reduction in pathogenic growth. This study demonstrates the use of Enterococcus species and plant proteins with several variations in treatment of MRSA biofilm. Results suggested that plant proteins and probiotics with its constituent inhibit resistant strains of Staphylococcus aureus and their synergistic effect has opened new ways to tackle emerging resistance of pathogens and to treat severe skin infections furthermore after assessment of Enterococcus as probiotics, this could be used in food industries as well.
Cytogenetic and genomic characterization of a novel tall wheatgrass-derived Fhb7 allele integrated into wheat B genome
Authors: Zhang Wei , Danilova Tatiana , Zhang Mingyi , Ren Shuangfeng , Zhu Xianwen , Zhang Qijun , Zhong Shaobin , Dykes Linda , Fiedler Jason , Xu Steven , Frels Katherine , Wegulo Stephen , Boehm Jeffrey , Cai Xiwen 
Published: August 2022

Abstract

A novel tall wheatgrass (Thinopyrum elongatum, genome EE)-derived Fhb7 allele, designated Fhb7The2, was identified and integrated into the wheat B genome through a small 7B-7E translocation (7BS·7BL-7EL) involving the terminal regions of the long arms. Fhb7The2 conditions significant Type II resistance to Fusarium head blight (FHB) in wheat. Integration of Fhb7The2 into the wheat B genome makes this wild species-derived FHB resistance gene usable for breeding in both common and durum wheat. By contrast, other Fhb7 introgression lines involving wheat chromosome 7D can be utilized only in common wheat breeding programs, not in durum wheat. Additionally, we found that Fhb7The2 does not have the linkage drag of the yellow flour pigment gene that is tightly linked to the decaploid Th. ponticum-derived Fhb7 allele Fhb7Thp. This will further improve the utility of Fhb7The2 in wheat breeding. DNA sequence analysis identified 12 single nucleotide polymorphisms (SNPs) in Fhb7The2, Fhb7Thp, and another Th. elongatum-derived Fhb7 allele Fhb7The1, which led to seven amino acid conversions in Fhb7The2, Fhb7Thp, and Fhb7The1, respectively. However, no significant variation was observed in their predicted protein configuration as a glutathione transferase. Diagnostic DNA markers were developed specifically for Fhb7The2. The 7EL segment containing Fhb7The2 in the translocation chromosome 7BS·7BL-7EL exhibited a monogenic inheritance pattern in the wheat genetic background. This will enhance the efficacy of marker-assisted selection for Fhb7The2 introgression, pyramiding, and deployment in wheat germplasm and varieties.