bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forest Bathing and Hiking benefits for mental health during the Covid-19 pandemic in Mediterranean regions
Authors: Muro Anna , Mateo Corel , Parrado Eva , Subirana-Malaret Montse , Moya Montserrat , Garriga Adrià , Canals Josep , Chamarro Andrés , Sanz Antoni 
Published: April 2022

Abstract
Forest Bathing (FB) is a nature-therapeutic modality that has evidenced positive effects on individuals’ mental health and it is also considered a sustainable touristic activity. However, its benefits have mainly been studied in Asian biomes and more cross-cultural research is needed to evaluate if its benefits are also generalizable to other forests and biomes of the world, such as the Mediterranean. Accordingly, 86 healthy adults of the general population were assessed before and after a FB near Barcelona (Spain) during the COVID-19 pandemic. A control hiking-group of participants was also analysed to contrast the FB effects on anxiety, affect, mood states and mindfulness. Results have shown that the guided practice of FB in Mediterranean-Catalan forests enhances significanlty mindfulness states and positive affect and reduces anxiety and negative affect, with effect sizes being large to very large. Hiking also induced significant changes in all variables tested, but FB showed higher effect sizes. An exploratory analysis also revealed a different profile of the FB participants compared to the hiking practitioners, being highly educated women living in urban areas and with lower basal levels of psychological wellbeing. Accordingly, it is concluded that both Mediterranean FB and hiking (to a lesser degree), might be cost-effective strategies to promote and restore psychological well-being after the Covid-19 pandemic and to promote sustainable tourism in Mediterranean biomes of the European forested and protected areas.
Highway Lane Detection and Vehicle Monitoring
Authors: Satpute Vishal , Das Siddharth , Rexy Joshin 
Published: April 2022

Abstract
It is a well-known fact that perception of road (lane) plays a vital role in assisting drivers by using software that can convey them the possible risks in their surroundings. Lane lines are a significant factor of signaling traffic flow. The strategy of this work is to prevent car accidents on highways mainly due to rash driving that consists of sudden lane departure or not keeping up with the lane in case of curved roads. Hence, we propose a method to reduce these crashes by mounting a calibrated camera on the vehicle which will continuously keep track of the view in front of vehicle and deliver certain relevant warnings to the driver in case of any rash driving or fatal situations. Also, it can be helpful for self-driving cars by providing a wide range of possibilities to learn by software and move vehicle on its own in the lane. We employ image processing techniques to detect and solve some of the most prevalent roadway concerns. The problem statement is broken down into basic building blocks to better understand the comprehensive list of potential techniques within this framework. It comes with a decision model that will provide the driver the required cautions.
Bioinformatic approach to biological processes and metabolic pathways of language gene ROBO1 and co-expressed genes
Authors: Limón-Fernández Héctor Adrián 
Published: April 2022

Abstract
Multiple genes have been associated with normal and abnormal development of brain and language processes; such is the case of ROBO1. It is relevant to clarify the biological processes and metabolic pathways to which this gene contributes and those that are co-expressed with it. The purpose of this study was to describe the biological processes and metabolic pathways involved in the co-expression of ROBO1 in human brain tissue. The data collected for this study were taken from transcriptomics databases (AHBA, SEEK and COXPRESdb), to later elaborate interactomes (STRING) and a determination of the most relevant biological processes and metabolic pathways (DAVID) in the co-expression of ROBO1. The biological processes of axon guidance, neuronal projection and neuronal migration were found to be the ones with the greatest coincidence and significance; in addition to the metabolic pathway of endocytosis. It is concluded that the endocytosis pathway is relevant for the projection and guidance of axons, as well as the formation of neural networks.
Underweight children are agile but lack power
Authors: Verbecque Evi , Coetzee Dané , Smits-Engelsman Bouwien 
Published: April 2022

Abstract
Given the knowledge gap in literature on the impact of undernutrition on muscular power and agility in school-aged children, the aim of this study was to compare physical fitness in such underweight- and normal weight children. In this cross-sectional study, 853 children were included (459 boys; mean age: 9.2 (1.8) years). The children were grouped according to their BMI-for-age-and-sex: normal weight (-1≤z-score<2) and underweight (z-score<-1). Within the underweight group, three thinness subgroups were composed: grade 1 (-2≤z-score<-1), grade 2 (-3≤z-score<-2) and grade 3 (z-score<-3). Their agility, muscular endurance and power were assessed with the PERFormance FITness test battery (PERF-FIT). Regardless the country they lived in, the underweight children showed better agility (p=0.012) and muscular endurance (p=0.004) than those with normal weight. They presented with lower muscular power than the normal weight group, shown by significantly shorter overhead throwing distances (p=0.017) and less standing long jump peak power (p<0.001). The standing long jump peak power decreased further with increasing thinness grade (p=0.027).Conclusion: Underweight children are more agile, but have lower muscular power compared to their normal weight peers. Its relationship with motor competence and physical activity, necessitates attention for tackling muscular strength deficiencies in these children, enabling them to meet the basic requirements for a healthy life style later in life.
Skeletal Muscle Proteostasis Promotes Central Nervous System Rejuvenation and Reduces Neuroinflammation during Aging and Neurodegenerative Disease
Authors: Matthews Ian , Birmbaum Allison , Gromova Anastasia , Coutinho Kristen , McGraw Megan , Patterson Dalton , Banks Macy , Nobles Amber , Nguyen Nhaty , Miranda Helen , Spada Albert La , Cortes Constanza 
Published: April 2022

Abstract
Skeletal muscle has recently arisen as a novel regulator of Central Nervous System (CNS) function and aging, secreting bioactive molecules known as myokines with proteostasis and metabolism-modifying functions in targeted tissues, including the CNS. Here we report the generation of a novel transgenic mouse with enhanced skeletal muscle proteostasis via moderate overexpression of master regulator of proteostasis and lysosomal function Transcription Factor E-B. We have discovered that the resulting enhanced muscle proteostasis function significantly ameliorates proteotoxicity, reduces neuroinflammation and promotes transcriptional remodeling of the aging CNS, preserving cognition and memory in aging mice. Enhancing skeletal muscle proteostasis also reduces neuroinflammation and accumulation of tau-associated pathological hallmarks in a mouse model of tau pathology. Our results implicate maintenance of skeletal muscle proteostasis throughout aging to direct regulations of the aging CNS metabolism and function, and suggest that skeletal-muscle originating factors may act as novel therapeutic targets against age-associated neurodegenerative diseases.
Integrating network pharmacology and in vitro experiment to investigate the protective effects of semen cuscutae flavonoid on sertoli cells and blood-testis barrier in male infertility under heat stress
Authors: Liu Chenxiao , Hu Suqin , Liu Dianlong , Xu Yahui , Hu Ke , Guo Jian 
Published: April 2022

Abstract
Background: Semen cuscutae is one of the common drugs used in treating male infertility (MI) in Traditional Chinese Medicine (TCM), and semen cuscutae flavonoid (SCF) is the main active component of semen cuscutae. The mechanism of SCF on sertoli cells (SCs) and blood-testis barrier (BTB) in male infertility under heat stress have not been clarified yet. Methods: Here, network pharmacology were used to explore the targets and signaling pathways of SCF in the treatment of MI. Then, in vitro experiments were integrated to verify the results of network pharmacology. In the in vitro experiments, the cell viability was measured by performing the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Ki67 and AR expression were detected by performing immunohistochemistry staining, and AR, Akt, CK-18, and Occludin levels were measured using western blotting. The Akt inhibitor assay was further performed to elucidate the signaling pathway underlying the function of SCF.Result: The data showed a total of 136 potential therapeutic targets of SCF, including HPS90AA1, SRC, JUN, TP53, MAPK1, AKT1, etc., were identified. In vitro experiments suggested that SCF significantly improved the viability of SCs, upregulated the expression of AR, Akt, Occludin, and Ki67, and downregulated the expression of CK-18. After adding the Akt inhibitor to block the PI3K-Akt signaling pathway, the effect of SCF decreased, indicating that SCF regulates the PI3K-Akt signaling pathway via AR, and then regulates occludin, Ki67, and CK-18. Gene ontology (GO) enrichment and Kyoto Encyclopedia of genes and genomes (KEGG) pathway analyses also suggested that SCF regulates cell proliferation and differentiation mainly via the PI3K-Akt signaling pathway. Conclusions: We suggested that the active components of SCF can promote AR expression, and regulate the PI3K-Akt signaling pathway and its downstream factors (occludin, Ki67, and CK-18), to regulate the viability, proliferation, differentiation, and BTB permeability of SCs, ultimately protecting reproductive ability.
The Percentage of ALK-Positive Cells and the Efficacy of First-Line Alectinib in Advanced Non-small Cell Lung Cancer: Is it a novel factor for stratification? (Turkish Oncology Group Study)
Authors: Hizal Mutlu , Bilgin Burak , Paksoy Nail , Atcı Muhammed Mustafa , Kahraman Seda , Kılıçkap Saadettin , Keskinkılıç Merve , Ayhan Murat , Eren Önder , Mustafayev Fatma Nihan Akkoç , Yaman Şebnem , Bayram Ertuğrul , Ertürk İsmail , Özcan Erkan , Korkmaz Mustafa , Akagündüz Baran , Erdem Dilek , Telli Tuğba Akın , Aksoy Asude , Üskent Necdet , Baytemür Naziyet Köse , Gülmez Ahmet , Aydın Dinçer , Şakalar Teoman , Arak Hacı , Tatlı Ali Murat , Ergün Yakup , Ak Naziye , Ünal Çağlar , Özgün Mehmet Alpaslan , Yalçın Bülent , Öztop İlhan , Algın Efnan , Sakin Abdullah , Aydıner Adnan , Yumuk Perran Fulden , Şendur Mehmet Ali Nahit 
Published: April 2022

Abstract
Introduction: Alectinib is an effective second-generation ALK tyrosine kinase inhibitor (TKI) used in the first-line treatment of patients with advanced ALK-positive NSCLC. Recent studies demonstrated that the percentage of ALK-positive tumor cells in patient groups receiving crizotinib might affect outcomes. This study aimed to investigate whether the percentage of ALK-positive cells had a predictive effect in patients with advanced NSCLC who received first-line Alectinib as ALK-TKI.Materials and Methods: This retrospective study included patients with advanced-stage NSCLC who received alectinib as a first-line ALK-TKI and whose percentage of ALK-positive cells was determined by FISH at 27 different centers. Patients who received any ALK-TKI before alectinib were not included in the study. Patients were separated into two groups according to the median (40%) value of the percentage of ALK-positive cells (high-positive group ≥40% and low-positive group <40%). The primary endpoint was PFS, and the secondary endpoints were OS, ORR, and PFS of the subgroups based on different threshold values for the percentage of ALK-positive cells.Results: 211 patients were enrolled (48.3% female, 51.7% male) to study. 37% (n=78) of the patients had received chemotherapy previously. After a median of 19.4 months of follow-up, the median PFS was not reached in the high-positive group (n=113) but it was 10.8 months in the low-positive group (n=98) (HR: 0.39; 95% CI, 0.25-0.60, p<0.001). The median OS in the high positive group was not reached, whereas it was 22.8 months in the low positive group (HR: 0.37; 95% CI, 0.22-0.63, p<0.001). ORR was significantly higher in the high-positive group (87.2 vs. 68.5%; p=0.002). According to the cutoff values of <20%, 20-39%, 40-59%, and ≥60%, the median PFS was 4.5, 17.1, and 26 months, respectively, and could not be reached in the ≥60% group.Conclusion: Our study demonstrated that the efficacy of alectinib varies significantly across patient subgroups with different percentages of ALK-positive cells. If these findings are prospectively validated, the percentage of ALK-positive cells may be used as a stratification factor in randomized trials comparing different ALK-TKIs.
Geochemical Provenance of an Indo-Arabian Stone Anchor from Manikapatna, East Coast of India: Implications for Medieval Maritime Trade of India
Authors: Tripati Sila , Ray Jyotiranjan S. , Behera Rudra Prasad , Babu Prakash , Mahala Milan Kumar , Kocherla Murali , Khedekar Vijay 
Published: April 2022

Abstract
India is one of the oldest maritime nations in the world, and the overseas contacts date back to the third millennium BCE. Besides several archaeological vestiges, numerous stone anchors of various types have been documented during maritime archaeological explorations along the Indian littoral. During a recent maritime archaeological exploration, a broken Indo-Arabian stone anchor, of the Medieval period, was discovered along the Manikapatna coast of Odisha, Indian eastern littoral. In an attempt to determine the provenance of the anchor, we carried out a detailed petrographic, geochemical (major/trace elements) and Sr-Nd isotopic investigation. The results of our study reveal that the stone of the anchor had been cut out of a geologically young, vesicular, alkali basalt lava flow. Source fingerprinting done using petrographic, geochemical and isotopic data, suggests that contrary to the general perception, the anchor rock did not come from any local rock formations. All data point to the most likely scenario that it was sourced from one of the Deccan Traps lava flows of Palitana in the Saurashtra region of Gujarat, western India. This confirms the Medieval maritime trading between western and eastern Indian states.
Effect of heating on protein denaturation, water state, microstructure, and textural properties of Antarctic krill (Euphausia superba) meat
Authors: Sun Peizi , Lin Junxin , Ren Xiang , Zhang Biao , Liu Jiaxin , Zhao Yanfen , Li Dongmei 
Published: April 2022

Abstract
Antarctic krill (Euphausia superba) is an important source of biomass and high-quality protein. However, heat treatment of Antarctic krill negatively impacts its quality and compromises its utilization in the food industry. This study aimed to investigate the mechanisms underlying changes in Antarctic krill meat characteristics and physicochemical properties treated at different temperatures and holding times. Findings indicate that at higher temperatures and holding times, hardness and cooking loss of Antarctic krill meat increased dramatically. The low-field nuclear magnetic resonance (LF-NMR) analysis revealed that the loss of immobile water increased, whereas SDS-PAGE analysis showed that the content of myosin heavy chain decreased significantly, and that protein degradation occurred. Fourier transform infrared spectroscopy (FT-IR) and intrinsic fluorescence spectra indicated that α-helix motifs were transformed into β-sheets, and that more hydrophobic groups were exposed. The scanning electron microscopy (SEM) results showed that Antarctic krill meat formed corrugated folded regions after heat treatment without forming a three-dimensional water-entrapped structure, which led to significant water loss, resulting in rapid deterioration Antarctic krill meat. These results provide the basis for a deeper understanding of processing characteristics of Antarctic krill meat.
Ablation of CX3CR1-expressing Immune Cells after Radiation Attenuates Tumor Progression and Sensitizes Cancer Cells to S-phase Specific Chemotherapy
Authors: Ben-Mordechai Tamar , Lawrence Yaacov R. , Symon Zvi , Shimoni-Sebag Ariel , Amit Uri 
Published: April 2022

Abstract
Introduction: Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1) is present on a subset of the immune cells in the tumor microenvironment (TME) and plays an essential and diverse role in cancer progression. However, its potential function in the irradiated TME remains unknown.Materials and Methods: Mouse lung cancer model was performed by subcutaneously inoculating Lewis Lung Carcinoma (LLC) cells expressing luciferase (Luc-2) and mCherry cells in CX3CR1GFP/GFP , CX3CR1DTR/+ , and wild–type (WT) mice. Bioluminescence imaging, clonogenic assay, and flow cytometry were used to assess tumor progression, proliferation, and cell composition after radiation.Results: Radiation provoked a significant influx of CX3CR1-expressing immune cells, notably monocytes, into the TME. Co-culturing irradiated LLC cells with CX3CR1-deficient monocytes and macrophages resulted in reduced clonogenic survival and increased apoptosis of the cancer cells. Interestingly, depletion of CX3CR1 in macrophages led to a redistribution of the irradiated LLC cells in the S-phase, parallel to increased expression of cyclin E1, required for cell cycle G1/S transition. In addition, deletion of CX3CR1 expression in macrophages altered the cytokine secretion with a decrease of interleukin-6, a crucial mediator of cancer cell survival and proliferation. Next, LLC cells were injected subcutaneously into CX3CR1DTR/+ mice, sensitive to diphtheria toxin (DT), and WT mice. After injection, tumors were irradiated with 8Gy, and mice were treated with DT, leading to conditional ablation of CX3CR1-expressing cells. After three weeks, CX3CR1 depleted mice displayed reduced tumor progression. Furthermore, combining the S-phase specific chemotherapy gemcitabine with CX3CR1 cell ablation resulted in additional attenuation of tumor progression.Conclusion: CX3CR1-expressing mononuclear cells invade the TME after radiation therapy in a mouse lung cancer model. CX3CR1 cell depletion attenuates tumor progression following radiation and sensitizes the tumor to S–phase-specific chemotherapy. Thus, we propose a novel strategy to improve radiation sensitivity by targeting the CX3CR1-expressing immune cells.
Safety and efficacy of allogeneic hematopoietic stem cell transplantation in R/R B-NHL patients with disease progression after CAR-T therapy
Authors: Cong Mengya , Ai Sicheng , Kang Liqing , Jin Mao , Zhu Ying , Li Caixia , Jin Zhengming , Yu Lei , Wu Depei , Huang Haiwen 
Published: April 2022

Abstract
Background: R/R B-NHL patients with disease progression after CAR-T therapy have poor prognosis and no standard chemotherapy regimen has been defined. We aimed to explore whether receiving allo-HSCT could improve the outcomes of R/R B-NHL patients who have disease progression after CAR-T therapy.Methods: From October 2017 to June 2021, we retrospectively report outcomes of R/R B-NHL patients with disease progression after CAR-T cell treatment, then receiving allo-HSCT. Results: Among 9 patients, 4 were males and 5 females, with a median age of 44 (27-52) years. 5 patients were diagnosed refractory/relapsed diffuse large B cell lymphoma, 1 patient was Burkitt lymphoma, 1 was B lymphoblastic lymphoma, 1 was transformed DLBCL and 1 was gray zone lymphoma. 8/9 donors were haploidentical family member, 1/9 donor was identical sibling. The median time from CAR-T treatment to transplantation was 2.3 (1.0-12.3) months. 9/9 patients obtained complete engraftment. The median time of neutrophil implantation was 12 (11- 19) days, and 13 (9-20) days of platelet implantation. The median follow-up was 7.9 (3.4-48.6) months. 4/9 patients received CR and 1/9 patient received PR during the follow up. The objective response rate (ORR) was 55.6%. The six-months overall survivaln (OS) and progression-free survival (PFS) were 66.7% and 33.3%, respectively. 1 case experienced acute graft-versus-host disease（aGVHD）grade Ⅱ, 1 case with aGVHD grade Ⅲ. Among 5 survivals, localized chronic GVHD occurred in 1 patient. During the follow up, four patients have died and the causes were disease relapses and progressions (2 patients), acute renal failure (1 patient), severe pulmonary infection (1 patient). No-relapse mortality was 22.2%.Conclusion: The present study demonstrated that allo- HSCT is a feasible and safe choice with favorable outcome for R/R B-NHL patients with disease progression after CAR-T therapy.
Surgery versus radiotherapy in octogenarians with stage Ⅰa non‑small cell lung cancer: Propensity score‑matching analysis of SEER database
Authors: Ni Lianfang , lin Gang , Zhang Zhigang , Sun Dan , Liu Zhonghui , Liu Xinmin 
Published: April 2022

Abstract
Purpose
To compare the overall survival (OS) and cancer-specific survival (CSS) of surgery with radiotherapy in octogenarians with stage Ⅰa non–small cell lung cancer (NSCLC).
Methods
Patients aged ≥ 80 years with clinical stage Ⅰa (T1N0M0) NSCLC from 2012 to 2017 were identified from the population-based Surveillance, Epidemiology, and End Results (SEER) database. Included patients were divided into surgery and radiotherapy group. Multivariate Cox regression was used to identify factors associated with survival. Propensity score‑matching (PSM) analysis was used to adjust treatment groups. OS and CSS were compared among groups by the Kaplan–Meier analysis.
Results
A total of 1641 patients were identified, with 46.0% received surgery and 54.0% radiotherapy. Compared with surgery, patients treated with radiotherapy were older, later diagnosed, and had more squamous cell carcinoma, unknown grade and increased tumor size. Radiotherapy was associated with a significantly worse OS as compared with surgery (hazard ratio 2.426; 95% CI, 2.003 to 2.939; P < .001). After PSM, the OS (P < 0.001) and CSS (P < 0.001) was higher in the surgery group. The 1-, 3-, and 5-year OS rates of surgery and radiotherapy group were 90.0%, 76.9%, 59.9%, and 86.0%, 54.3%, 28.0%, respectively. The 1-, 3-, and 5-year CSS rates of surgery and radiotherapy group were 94.5%, 86.1%, 78.0% and 90.7%, 74.5%, 61.0%, respectively. No differences in the OS (P = 0.146) and CSS (P = 0.675) were found between the matched surgery without lymph node examination (LNE) and radiotherapy group.
Conclusions
Surgery with lymph node dissection offers better OS and CSS than radiotherapy in octogenarians with stage Ⅰa NSCLC.