bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of the 2-deoxyglucose scaffold as a new chiral probe for elucidation of the absolute configuration of secondary alcohols
Authors: Trocka Alicja , Szwarc-Karabyka Katarzyna , Makowiec Sławomir , Laskowski Tomasz 
Published: July 2022

Abstract
Herein, we present the application of 2-deoxy-D-glucose derivatives as chiral probes for elucidation of the absolute configuration of chiral secondary alcohols. The probes are attached to the studied molecules via glycosylation reaction and the resulting products are examined by a set of standard 2D NMR experiments. The absolute configuration of an oxymethine carbon atom binding the probe is established on a basis of a set of diagnostic dipolar couplings (NOEs/ROEs). These correlations may be considered diagnostic due to a pronounced lack of conformational freedom of the formed glycosidic linkage. While the chance for an observation of the diagnostic signals is the highest when the resulting glycoside in an α-anomer. 2-deoxy-D-glucose was selected as a probe of choice since is it known to strongly prefer the formation of α-glycosides
Comprehensive proteome and phosphoproteome analysis reveals the protein characterization of invasive ductal and lobular carcinoma with specific molecular subgroup
Authors: Yang Ganglong , Zuo Chenyang , Fu Fangmeng , Gao Xiao-Dong , Lin Yuxiang , Wen Piaopiao , Chen Wenyan , Zhang Chairui , Xiao Han , Jiang Meichen , Zhou Xiaoman , Fujita Morihisa 
Published: July 2022

Abstract
Background
Breast cancer is one of the most frequently occurring malignant cancers worldwide. Invasive ductal carcinoma (IDC) and invasive lobular carcinoma (ILC) are the two most common histological subtypes of breast cancer. The prognosis and survival of the two subtypes were significantly different even with specific molecular subgroup. In this study, we aimed to deeply explore molecular characteristics and the relationship between IDC and ILC subtypes in same molecular subgroup of breast cancer using comprehensive proteomics and phosphoproteomics analysis.
Methods
Cancer tissues and noncancerous adjacent tissues (NATs) with the luminal subtype (ER- and PR-positive, HER2-negative) were obtained from paired IDC and ILC patients respectively. Label-free quantitative proteomics and phosphoproteomics methods were used to detect differentially expressed proteins and the phosphorylation status between 10 paired breast cancer and NATs. Then, bioinformatics analysis was performed to explore the difference between IDC and ILC subtypes, including the difference in protein expression levels and the degree of phosphorylation. Meanwhile, Kinase-Substrate Enrichment Analysis (KSEA) revealed the activation difference of kinases and their substrates between IDC and ILC.
Results
A total of 5,044 high-confidence proteins and 3,808 phosphoproteins were identified from breast cancer tissues. The protein phosphorylation level in ILC tissues was higher than that in IDC tissues. From the quantitative analysis of 1259 proteins and 560 phosphoproteins with high patient coverage, Histone H1.10, Complement C4-B and Crk-like protein were found to be significantly differentially expressed in the two subtype tissues from the proteomics analysis. Moreover, the differences in protein expression of Septin-2, Septin-9, Heterogeneous nuclear ribonucleoprotein A1 and Kinectin and their phosphorylation clearly distinguished IDC from ILC. In addition, differentially expressed proteins and differentially expressed phosphoproteins in IDC were primarily related to energy metabolism and MAPK pathway, while ILC subtypes were more closely involved in the AMPK and p53/p21 pathways. Furthermore, the kinomes from IDC were primarily significantly activated in the CMGC (Cyclin-dependent kinases, Mitogen-activated protein kinases, Glycogen synthase kinases and CDK-like kinases) groups.
Conclusions
Our research provides insights into the molecular characterization of IDC and ILC and contributes to discovering novel targets for further drug development and targeted treatment.
Effects of Nanopillars and Surface Coating on Dynamic Traction Force
Authors: Pang Stella , Cheng Yijun 
Published: July 2022

Abstract
Extracellular matrix serves as a structural support for cells and provides biophysical and biochemical cues for cell migration. Topography, material, and surface energy can regulate cell migration behaviors. The responses of MC3T3-E1 cells, including migration speed, morphology, and spreading on various platform surfaces were investigated. Polydimethylsiloxane (PDMS) micropost sensing platforms with nanopillars, silicon oxide, and titanium oxide on top of the microposts were fabricated, and dynamic cell traction force during migration was monitored. The relationships between various platform surfaces, migration behaviors, and cell traction force were studied. Compared with flat PDMS surface, cells on silicon oxide and titanium oxide surfaces showed reduced mobility, less elongation, and larger cell area. On the other hand, cells on nanopillar surface showed more elongation, higher migration speed, and smaller cell area compared to cells on silicon oxide and titanium oxide surfaces. MC3T3-E1 cells on microposts with nanopillars exerted larger traction force than those on microposts without nanopillars, as well as having more extensions of filopodia and long protrusions. Understanding the relationships between platform surface condition, migration behavior, and cell traction force can potentially lead to better control of cell migration in biomaterials capable of promoting tissue repair and regeneration.
A Phase I-II multicenter trial with Avelumab plus autologous dendritic cell vaccine in pre-treated mismatch repair-proficient (MSS) metastatic colorectal cancer patients. GEMCAD 1602 study
Authors: Español-Rego Marta , Fernandez-Martos Carlos , Elez Elena , Foguet Carles , Pedrosa Leire , Rodriguez Núria , Ruiz-Casado Ana , Pineda Estela , Cid Joan , Cabezon Raquel , Oliveres Helena , Lozano Miquel , Ginés Angels , Garcia-Criado Angeles , Ayuso Juan Ramon , Pages Mario , Cuatrecasas Miriam , Torres Ferran , Thomson Timothy , Cascante Marta , Benitez-Ribas Daniel , Maurel Joan 
Published: July 2022

Abstract
Background: Immune check-point blockade (ICB) has shown clinical benefit in mismatch repair-deficient/microsatellite instability high metastatic colorectal cancer (mCRC) but not in mismatch repair-proficient/microsatellite stable patients. Cancer vaccines with autologous dendritic cells (ADC) could be a complementary therapeutic approach to ICB as this combination has the potential to achieve synergistic effects. Methods: This was a Phase I/II multicentric study with translational sub-studies, to evaluate the safety, pharmacodynamics and anti-tumor effects of Avelumab plus ADC vaccine in heavily pre-treated MSS mCRC patients. Primary objective was to determine the maximum tolerated dose and the efficacy of the combination. The primary end-point was 40% progression-free survival at 6 months with a 2 Simon Stage. Results: A total of 28 patients were screened and 19 pts were included. Combined therapy was safe and well tolerated. An interim analysis (Simon design first-stage) recommended early termination because only 2/19 (11%) patients were disease free at 6 months. Median PFS was 3.1 months [2.1 – 5.3 months] and overall survival was 12.2 months [3.2 – 23.2 months]. Stimulation of immune system was observed in vitro but not clinically. The evaluation of basal RNA-seq noted significant changes between pre and post-therapy liver biopsies related to lipid metabolism and transport, inflammation and oxidative stress pathways. Conclusions: The combination of Avelumab plus ADC vaccine is safe and well tolerated but exhibited modest clinical activity. Our study describes, for the first-time, a de novo post-therapy metabolic rewiring, that could represent novel immunotherapy-induced tumor vulnerabilities. Trial registration: ClinicalTrials.gov (NCT03152565)
BfmRS encodes a regulatory system involved in light signal transduction in the human pathogen Acinetobacter baumannii.
Authors: Mora Bárbara Perez , Giordano Rocío , Permingeat Valentín , Calderone Malena , Arana Natalia , Müller Gabriela , Rodriguez Ramiro E. , Krasauskas Renatas , Mussi María Alejandra 
Published: July 2022

Abstract
We have previously shown that Acinetobacter baumannii as well as other relevant clinical bacterial pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa, perceive and respond to light at 37°C, the normal temperature in mammal hosts. In this work, we present evidence indicating that the two-component system BfmRS transduces a light signal in A. baumannii at this temperature, showing selective involvement of the BfmR and BfmS components depending on the specific cellular process. In fact, both BfmR and BfmS participate in modulation of motility by light, while only BfmR is involved in light regulation of desiccation tolerance in this microorganism. Neither BfmR nor BfmS contain a photoreceptor domain and then most likely, the system is sensing light indirectly. Intriguingly, this system inhibits blsA expression at 37°C, explaining previous results indicating that BlsA only functions at moderate temperatures 1–3, and thus governing the response to light at 37°C. Overall, we provide experimental evidence on a new biological function of this multifaceted system that broadens our understanding of A. baumannii’s physiology and responses to light.
Novel transcriptomic events discovery in esophageal cancer via alignment-free protocol
Authors: Xue Wenhua , Li Lifeng , Shen Zhibo , Zhao Jie , Liu Kangdong , Chen Kuisheng 
Published: July 2022

Abstract
ABSTRACT
Background
The combination of epigenetics, genomic variation, and transcriptional regulatory variation determines the identity of cancer cells. The differences determining the phenotype are frequently represented in transcriptome expression differences or peptides and proteins with generated aberrant structures. As a result, RNA-seq data is a valuable resource for discovering pathogenic variations.

Methods
We use k-mer technology to comprehensively capture pathogenic variations, which overcomes the constraints of typical NGS analysis approaches that rely on reference sequences and the alignment process.

Results
In esophageal cancer patients, we discovered high-frequency variations from various places (introns, exons, intergenic regions, repeats) and forms (splicing, polyadenylation, fusion, etc.). We investigated the significance of overlooked events in typical transcriptomics pipelines as indicators for tumor prediction, prognosis, tumor neoantigen prediction, and their relationship with the immune microenvironment. Unannotated lincRNA, new splice variants, repetitive sequences, and pathogenic microbial RNA all help to explain the process of esophageal cancer, according to our findings.

Conclusion
We anticipate that our reference/mapping-free technique will assist differential RNA-seq analysis of tumor/normal sample sets, offering a broader network for significant cancer-related events.
Effect of measurement errors in consumption expenditure on catastrophic health spending in India
Authors: Mohanty Sanjay K , Sharma Santosh Kumar , Dwivedi Laxmikant , Saggurti Niranjan 
Published: July 2022

Abstract
BackgroundIndia’s National Sample Survey (NSS), through its annual multi-subject and multi-round surveys, collects reliable data on morbidity, health care, and health spending. It also collects data on household consumption expenditure through a single question, which is used to explain the economic gradient in health outcomes. Literature suggests that using a single question to measure consumption expenditure results in measurement errors. In this paper, we examine the effect of measurement errors of household consumption expenditure on estimates of catastrophic health expenditure in India.MethodData from the 68th round of the consumption survey, 2011-12, and the 71st round of the health survey, 2014, carried out by the National Sample Survey (NSS) were used in the analysis. The consumption survey canvassed a detailed schedule on consumption and interviewed 101,651 households, whereas the health survey interviewed 65,932 households in the country. Descriptive statistics, estimates of catastrophic health expenditure and logistic regression models were used in the analysis. ResultResults suggest that the NSS health survey under-estimated monthly per capita expenditure (MPCE) in India by 32%. The level of underestimation is significant across the states of the country. Without adjusting for the level of underestimation, the incidence of catastrophic health expenditure (CHE) was overestimated at least by 3% in India. The pattern was similar with respect to the intensity of CHE. Though the predictors of CHE are similar with both the methods, the predicted probabilities of incurring CHE were significantly higher in unadjusted estimates of CHE across household characteristics. ConclusionMonthly per capita consumption expenditure in NSS-based health surveys is underestimated. It is recommended that the future rounds of NSS-based health surveys should include disaggregated questions on consumption expenditure and researchers may need to adjust the consumption expenditure while using such information in estimating CHE.
Anaplasma marginale multistrain infection in bovines under natural conditions correlates with transovarial transmission and vector competence in Rhipicephalus microplus ticks
Authors: Fournière Sofía , Guillemi † -Eliana Carolina , Paoletta Martina Soledad , Sarmiento Néstor Fabián , Farber Marisa Diana 
Published: July 2022

Abstract
Background
Anaplasma marginale, a gram-negative obligate intracellular microorganism, infects erythrocytes of ruminants and other host species from tropical and subtropical world regions. This pathogen of importance for cattle production, is known to be transmitted mechanically and through hematophagous parasites, mainly by Rhipicephalus microplus ticks. Even though transovarial transmission of A. marginale has been previously reported in R. microplus ticks, there is no evidence of A. marginale transmission from the resulting unfed larvae to a susceptible host. The aim of the present study was to evaluate the transovarial transmission of A. marginale in the R. microplus tick under natural conditions and to study the vector competence of the infected hatched larvae.
Methods
Engorged female R. microplus ticks were sampled from A. marginale positive bovines from an enzootic area in Argentina, and larvae were obtained from a subgroup of these females. After identifying A. marginale in the females and their larvae, selected ticks collected from a single bovine were used. After spawn, hatched larvae were seeded on a susceptible healthy bovine and another subgroup of engorged R. microplus ticks were subjected to dissection to recover the salivary glands and ovaries for A. marginale identification. Anaplasma marginale was detected by PCR and molecularly characterized by means of cloning and sequence all msp1α fragments while genotyping.
Results
A. marginale was detected in naturally infected bovines, in different organs of engorged R. microplus females that were parasitizing them, and in the larvae obtained from these females. A. marginale infection of the susceptible bovine was corroborated both from DNA detection and from compatible structures in a blood smear. The characterization of A. marginale msp1α genotypes involved throughout the infection process provided relevant information about the dynamics of the transmission process.
Conclusions
The results demonstrate the transovarial transmission of A. marginale in R. microplus ticks under natural conditions and, through an experimental infection assay, the vector competence of the infected R. microplus larvae to transmit A. marginale to a susceptible bovine. The high genetic diversity of A. marginale in natural transmission settings seems to be one of the key factors for the transovarial transmission to occur.
Temperature Response of Metabolic Activity of an Antarctic Nematode
Authors: Robinson Colin M. , Hansen Lee D. , Xue Xia , Adams Byron J. 
Published: July 2022

Abstract
Because of climate change, the McMurdo Dry Valleys of Antarctica (MCM) have experienced increased summer temperatures and surface ice and snow melting events. In response to these environmental changes, some nematode species in the MCM have experienced steady population declines over the last three decades, but Plectus murrayi, a mesophilic nematode species, has responded with a steady increase in range and abundance. To determine how P. murrayi responds to increasing temperatures we measured metabolic heat and CO2 production rates and calculated O2 consumption rates as a function of temperature at 5°C intervals from 5 to 50°C. Heat, CO2 production, and O2 consumption rates increase approximately exponentially up to 40°C, a temperature never experienced in their polar habitat. Metabolic rates decline rapidly above 40°C and are irreversibly lost at 50°C due to thermal stress and mortality. Caenorhabditis elegans, a much more widespread nematode that is found in more temperate environments reaches peak metabolic heat rate at just 27°C, above which it experiences high mortality due to thermal stress. At temperatures from 10 to 40°C, P. murrayi produces about 6 times more CO2 than the O2 it consumes, a respiratory quotient indicative of either acetogenesis or de novo lipogenesis. No potential acetogenic microbes were identified in the P. murrayi microbiome. We, therefore, conclude that P. murrayi is producing increased CO2 as a byproduct of de novo lipogenesis. This phenomenon, in conjunction with increased summer temperatures in their polar habitat, will likely lead to increased CO2 production, population abundance, and range expansion.
Biotreatment of azo dye containing textile industry effluent by a developed bacterial consortium immobilised on brick pieces in an indigenously designed packed bed biofilm reactor
Authors: Agrawal Shweta , Tipre Devayani , Dave Shailesh R. 
Published: July 2022

Abstract
This study highlights the development of a lab-scale, indigenously designed; Packed-Bed Biofilm Reactors (PBBR) packed with brick pieces. The developed biofilm in the reactor was used for the decolourisation and biodegradation of the textile industry effluent. The PBBR was continuously operated for 264 days, during which 301 cycles of batch and continuous treatment were operated. In batch mode under optimised conditions, more than 99% dye decolourisation and ≥ 92% COD reduction were achieved in 6 h of contact time upon supplementation of effluent with 0.25 g L− 1 glucose, 0.25 g L− 1 urea, and 0.1 g L− 1 phosphates. A decolourisation rate of 133.94 ADMI units h− 1 was achieved in the process. PBBR, when operated in continuous mode, showed ≥ 95% and ≥ 92% reduction in ADMI and COD values. Subsequent aeration and passage through charcoal reactor assisted in achieving ≥ 96% reduction in COD and ADMI values. An overall increase of 81% in dye-laden effluent decolourisation rate, from 62 mg L− 1 h− 1 to 262 mg L− 1 h− 1, was observed upon increasing the flow rate from 18 mL h− 1 to 210 mL h− 1. Dye biodegradation was determined by UV-Vis and FTIR spectroscopy and toxicity study. SEM analysis showed the morphology of the attached-growth biofilm.
Circularly Polarized Printed MIMO Antenna for 5G Applications
Authors: Gangula Acharya , challa Rama Krishna , gaddam Nandini , Karji Rihana Begum 
Published: July 2022

Abstract
This study provides a proposal for printed circularly polarized MIMO 5G antenna with four ports that are suitable for enhanced impedance bandwidth. The proposed circularly polarized MIMO antenna is designed at 28 GHz. The complementary split ring rectangular resonator with a defective ground plane is used to support the array, which is placed on a FR4 epoxy substrate that is 40 mm × 40 mm × 0.8 mm in physical size. The MIMO 5G antenna components are structured in an orthogonal arrangement with four identical patches that have a triangular cut. By integrating triangular cuts on rectangular patch and inset feeding techniques, the the designed antenna work across the whole frequency range with good impedance matching, increased gain, and with 1.5 GHz axial ratio bandwidth for circular polarization. The circular polarized MIMO 5G antenna that has been proposed achieves a gain of 5 dBi over bandwidth of 7GHz. The circularly polarized MIMO antenna would cover 5G bands between 25.5 GHz and 32.5 GHz and it provides an axial ratio bandwidth of 1.5 GHz from 27.25GHz to 28.75GHz, which will be deployed in the U.S. and Canada. In addition, a performance and analysis of the MIMO performance measures for the proposed architecture have been presented. An antenna prototype is built and tested to validate simulation results.
Influence of smoking on the effectiveness of at-home bleaching with 22% carbamide peroxide: A cohort study
Authors: Silva Rodrigo Rohenkohl , De Carli João Paulo , Pecho Oscar Emilio , Collares Kauê Faria , Bona Alvaro Della , Meireles Sônia Saeger , Júnior João Renato Dieterich , Benetti Paula 
Published: July 2022

Abstract
ObjectivesTo evaluate the effect of smoking on tooth whitening and color change after at-home bleaching.Materials and methodsThis is a cohort study evaluating two groups of participants: NS- non-smokers (n = 24) and S- smokers (n = 16). All subjects were treated with at-home bleaching using 22% carbamide peroxide (CP) in individual trays for 1 h per day, during 14 days. The color was evaluated before bleaching (D0-baseline), and one day (D1), 15 days (D15), and one month (D30) after bleaching. The color coordinates were measured using the VITA Easyshade™ spectrophotometer and the whiteness index (WID- Whiteness Index for Dentistry) and color differences (ΔE00) for S and NS at different times were calculated. Color changes between S and NS subjects for specific teeth (CI- Central Incisors, LI- Lateral Incisors, and C- Canines) were analyzed using Mann-Whitney test (α=0.05).ResultsThere was no significant difference of WID between S and NS subjects at baseline (D0). NS participants showed higher WID than S at D1, D15, and D30 (p < 0.05). NS showed higher ΔWID for CI and C between D0 and the other evaluation times (D1, D15, and D30) compared to S. Smokers showed higher darkening of CI than NS in ΔWID D30-D1 (p < 0.05). Color change (ΔE00) findings had the overall behavior similar to ΔWID. ΔWID and ΔE00 values for all evaluation times were within the acceptability threshold.ConclusionAlthough smoking showed some effects on the at-home bleaching treatment using 22% CP, teeth whiteness and color changes are clinically acceptable for both groups. Clinical significance: At-home bleaching is a widely performed treatment, however there is a lack of evidence using 22% carbamide peroxide gel in smokers. Although the central incisors showed significant darkening (lower WID) in smokers at 30 days after bleaching, the whitening index was considered clinically acceptable.