bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treatment outcomes and prognosis of patients with primary and acquired BRAF‐mutated non‐small cell lung cancer: A multicenter retrospective study
Authors: Wang Wenxian , Gu Xiaodong , Si Jinfei , Pu Xingxiang , Wang Liping , Chen Huafei , Xu Chunwei , Zhang Xiaoyan , Yuan Hongling , Lou Guangyuan , Shao Lan , Zhang Gu , Song Zhengbo 
Published: April 2022

AbstractThe incidence of primary and acquired BRAF mutations is low in non‐small cell lung cancer (NSCLC), with limited demographic and treatment outcome data available for this patient population. We evaluated lung cancer samples with programmed cell death ligand 1 (PD‐L1) information extracted from 12 051 cases (cohort A) of lung cancer from OncoPanscan™‐based sequencing of tissue (Genetron Health) and conducted retrospective multicenter data analysis using the database of Zhejiang Cancer Hospital and four other centers (cohort B, including 73 primary BRAF mutation and 14 acquired BRAF mutation cases) to compare treatment outcomes of patient groups with primary and acquired BRAF mutations. In cohort A, after propensity score analysis, 165 samples of NSCLC with BRAF mutations were screened along with 165 paired non‐BRAF mutation samples. We observed no significant differences in the proportion of samples with ≥1% PD‐L1 between BRAF and non‐BRAF mutant groups. The median progression‐free survival (mPFS) period in 13 patients with primary BRAF mutations receiving BRAF tyrosine kinase inhibitors (BRAF‐TKIs) was 7.0 months. The group with primary BRAF mutations receiving immune checkpoint inhibitor (ICI) combination chemotherapy had better PFS than those administered ICI monotherapy (14.77 months vs. 5.0 months, p = 0.025) and similar results were obtained for OS (unreached vs. 20.3 months, p = 0.013). For acquired BRAF mutations, mPFS of BRAF‐TKI, ICI‐based, and chemotherapy‐based regimens were 3.8, 1.5, and 1.9 months, respectively. Therefore, for patients with the primary BRAF V600E mutation, targeted therapy or immunochemotherapy could serve as effective treatment choices, while for those with acquired BRAF V600E, targeted drug therapy may remain the preferred solution in China.
Evaluation and analysis of methane adsorption capacity in deep‐buried coal seams
Authors: Zheng Yuannan , Jiang Bingyou , Ren Bo , Lin Hanyi , Tao Wenhan , Wang Shijv 
Published: April 2022

AbstractIn this work, the high‐pressure methane adsorption/desorption tests were conducted in four coal samples (from low‐rank bituminous coal to anthracite) under different temperature and pressure conditions to reveal the mechanism of desorption hysteresis effect in the deep‐buried coal seams. The experimental and fitting results show that the presence of free methane molecules in the adsorption phase results in an obvious difference between the measured methane adsorption amount with the actual adsorption capacity. The Langmuir, Langmuir–Freundlich (L–F) and Dubinin–Astakhov (D–A) models have their own advantages and disadvantages in fitting the experimental adsorption/desorption data. As residual adsorption capacity C fitted by the D–A model would be negative at high temperatures and lose the meaning of the constant, the Langmuir model is more suitable to describe methane desorption process. The residual adsorption capacity fitted by Langmuir model tended to decrease as the temperature increased until it is almost completely desorbed at 373 K. Meanwhile, because the adsorption sites with lower energy are gradually occupied by methane molecules in the high‐pressure stage, intermolecular forces increased and methane molecules are more easily desorbed. Therefore, the desorption hysteresis coefficient trends to increase exponentially with the decrease of pressure and temperature, which indicated that the original gas content of the coal seams may decrease with the increase of the burial depth, but the risk of coal and gas outburst accidents may increase. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.
The functional dissociation of posterior parietal regions during multimodal memory formation
Authors: Jablonowski Julia , Rose Michael 
Published: April 2022

AbstractThe incidental acquisition of multimodal associations is a key memory function for everyday life. While the posterior parietal cortex has been frequently shown to be involved for these memory functions, ventral and dorsal regions revealed differences in their functional recruitment and the precise difference in multimodal memory processing with respect to the associative process has not been differentiated. Using an incidental multimodal learning task, we isolated the associative process during multimodal learning and recollection. The result of the present functional magnetic resonance imaging (fMRI) study demonstrated that during both learning and recollection a clear functional differentiation between ventral and dorsal posterior parietal regions was found and can be related directly to the associative process. The recruitment of a ventral region, the angular gyrus, was specific for learning and recollection of multimodal associations. In contrast, a dorsal region, the superior parietal lobule, could be attributed to memory guided attentional processing. Independent of the memory stage, we assumed a general role for the angular gyrus in the generation of associative representations and updating of fixed association, episodic memory.
Racial and rural–urban disparities in cardiovascular risk factors among patients with head and neck cancer in a clinical cohort
Authors: Mukherjee Amrita , Wiener Howard W. , Griffin Russell L. , Lenneman Carrie , Chatterjee Arka , Nabell Lisle M. , Lewis Cora E. , Shrestha Sadeep 
Published: April 2022

AbstractBackgroundEvidence on distribution of cardiovascular disease (CVD) risk factors in patients with head and neck squamous cell carcinoma (HNSCC) is limited. We assessed disparities in prevalence and incidence of CVD risk factors in patients with HNSCC.MethodsElectronic health records (EHR) data on 2262 patients with HNSCC diagnosed between 2012 and 2018 at a NCI‐designated cancer center were included. Prevalence of CVD risk factors at baseline and incidence at 1‐year post HNSCC diagnosis were assessed using logistic and robust Poisson regression, respectively.ResultsAt baseline, 31.72% white patients with HNSCC had dyslipidemia, compared to 24.29% blacks (p < 0.008); diabetes was more prevalent in blacks (p < 0.027). Odds of ≥1 prevalent CVD clinical risk factor at baseline was lower in blacks (OR, 95%CI: 0.71, 0.54–0.93) and in rural patients (OR, 95%CI: 0.70, 0.58–0.85). At 1 year, risk of incident diabetes was higher in rural patients (RR, 95%CI: 1.63, 1.21–2.19).ConclusionsDemographic disparities were observed in distribution of CVD risk factors in patients with HNSCC.
Beyond fill and spill: Hydrological connectivity in a sub‐arctic bog‐fen‐tributary complex in the Hudson Bay Lowlands, Canada
Authors: Balliston Nicole , Price Jonathan S. 
Published: April 2022

AbstractPatterned bog and fen peatlands, which dominate the landscape in the Hudson Bay Lowlands (HBL), act as important water storage and conveyance features in this region. In spite of their hydrological importance, there are currently no studies that define and characterize the thresholds of bog‐fen‐tributary hydrological connectivity in the HBL or their relation to seasonal and annual changes in water fluxes. To this end, hydrological (i.e., streamflow and groundwater levels) and meteorological (i.e., precipitation, snow depth, evapotranspiration, and temperature) data were collected at a 4.8 km2bog‐fen‐tributary complex between 2007 and 2018. Connectivity thresholds were best characterized into three states (disconnected, connected, and high activity) that incorporated 41%, 47%, and 12% of the study period and 4%, 18%, and 78% of runoff, respectively. Runoff generally peaked in the spring due to snowmelt, while connectivity was highest in the peatlands in the fall months when precipitation exceeded evapotranspiration due to cooling temperatures. Warmer than average spring temperatures accelerated snowmelt rate faster than frost table thaw rate in the fen; this reduced the amount of meltwater that entered storage, increased drainage from bog to fen, and decreased overall connectivity in the unfrozen season. Cooler than average spring temperatures delayed bog connection and ground thaw; the late frost melt provided a source of water to the bogs after melt into the late spring and early summer. This study provides a basis for the modelling of peatland hydrological connectivity in the region in the drier conditions anticipated with climatic warming and regional resource extraction.
Effectiveness of enhanced recovery after surgery protocol in open gynecologic oncology surgery: A randomized controlled trial
Authors: Chiewhatpong Phasawee , Charoenkwan Kittipat , Smithiseth Kannika , Lapisatepun Warangkana , Lapisatepun Panuwat , Phimphilai Mattabhorn , Muangmool Tanarat , Cheewakriangkrai Chalong , Suprasert Prapaporn , Srisomboon Jatupol 
Published: April 2022

AbstractObjectiveTo examine the effectiveness of applying the recommended enhanced recovery after surgery (ERAS) protocol compared with our usual care in women with gynecologic malignancy undergoing elective laparotomy.MethodsFrom June 2020 to May 2021, 93 women with gynecologic cancers (cervix, endometrium, and ovary) undergoing elective laparotomy at our institution were randomly assigned into an intervention group (ERAS protocol, 46 women) or control group (usual care, 47 women). For the intervention group, each woman was brought through the pre‐specified ERAS protocol starting from preoperative counseling to postoperative management. For the control group, participants underwent routine standard care. The primary outcomes were length of hospital stay and postoperative pain.ResultsThe intervention group demonstrated shorter hospital stay by 20 h (47.48 h vs 67.17 h, P = 0.02) with lower postoperative pain score at postoperative day 0 (1.58 vs 4.00, P < 0.01) and day 1 (1.00 vs 2.67, P < 0.01) while having decreased opioid consumption (P < 0.01). The intervention group also had faster recovery of gastrointestinal function. Overall, good compliance to most of the ERAS pathway domains was obtained.ConclusionThe ERAS protocol demonstrates benefits on shortening hospital stay, reducing pain, and bowel function recovery without increasing complications in our population.Clinical Trial RegistrationThe present study was registered at clinicaltrials.gov (NCT04201626) on December 3, 2019. Initial participant enrollment began on June 1, 2020. Access through URL of the registration site: https://clinicaltrials.gov/ct2/show/NCT04201626?cond=ERAS&cntry=TH&draw=2&rank=3.
Comparison of two stitches versus one stitch for emergency cervical cerclage to prevent preterm birth in singleton pregnancies
Authors: Xu Zhi‐Min , Zhang Jun , Hong Xiao‐Lan , Liu Jing , Yang Zhen‐Zhen , Pan Mian 
Published: April 2022

AbstractObjectiveTo compare the efficacy of two stitches versus one stitch in women with emergency cervical cerclage.MethodsA retrospective case–control study of 26 women with singleton pregnancies who underwent emergency cervical cerclage before 26 weeks. A comparison was made between patients with two stitches versus one stitch at the time of cervical cerclage placement. The primary outcome was gestational age (GA) at delivery and preterm birth (PTB) before 37, 34, 32, 28, and 24 weeks.ResultsAverage GA at delivery in the two‐stitches group was significantly greater than in the one‐stitch group (32.71 ± 5.54 weeks vs 27.99 ± 4.70 weeks, P = 0.028). There were significant decreases in the incidence of spontaneous PTB at <34 weeks and less than 32 weeks in the two‐stitches group (P = 0.034; P = 0.013, respectively). The neonatal intensive care unit (NICU) admissions rate and length of stay in the NICU in the two‐stitches group were significantly reduced (P = 0.035 and P = 0.018, respectively). However, there was no significant difference in neonatal mortality between the two groups.ConclusionCompared with emergency cervical cerclage placement with one stitch, the procedure with two stitches can prolong the pregnancy and improve the neonatal prognosis more effectively.
Comparison of health‐related quality of life of women undergoing robotic surgery, laparoscopic surgery or laparotomy for gynecologic conditions: A cross‐sectional study
Authors: Kurt Gonul , Guvenc Gulten , Dede Murat , Yenen Mufit Cemal , Akyuz Aygul 
Published: April 2022

AbstractObjectiveTo assess and compare the health‐related quality of life of women undergoing robotic gynecologic surgery, laparoscopic gynecologic surgery or laparotomy for benign and cancerous conditions.MethodsCross‐sectional study design was used. The present study was carried out with 240 women, who underwent gynecologic surgery (robotic 48, laparoscopic 96, and laparotomy 96) in a tertiary care hospital. Instruments included a participant description questionnaire and Medical Outcomes Study Short Form‐36. The data were collected 4 weeks after surgery, at the first postoperative visit of women to the clinic. Pearson χ2 test, one‐way analysis of variance, and regression analysis were used to assess the data.ResultsOver half of the women in each group had surgery because of gynecologic cancer. All the subscale scores of Medical Outcomes Study Short Form‐36 were significantly higher in the robotic group than the other surgical groups (P < 0.05). Women in the robotic group had better quality of life in terms of both the physical component and the mental component after surgery.ConclusionsKnowledge of health‐related quality of life in the recovery period after surgery is important for healthcare providers to provide adequate preventive measures, information, and follow up.
Holographic optogenetic stimulation with calcium imaging as an all optical tool for cardiac electrophysiology
Authors: Junge Sebastian , Schmieder Felix , Sasse Philipp , Czarske Jürgen , Torres‐Mapa Maria Leilani , Heisterkamp Alexander 
Published: April 2022

AbstractAll optical approaches to control and read out the electrical activity in a cardiac syncytium can improve our understanding of cardiac electrophysiology. Here, we demonstrate optogenetic stimulation of cardiomyocytes with high spatial precision using light foci generated with a ferroelectric spatial light modulator. Computer generated holograms binarized by bidirectional error diffusion create multiple foci with more even intensity distribution compared with thresholding approach. We evoke the electrical activity of cardiac HL1 cells expressing the channelrhodopsin‐2 variant, ChR2(H134R) using single and multiple light foci and at the same time visualize the action potential using a calcium sensitive indicator called Cal‐630. We show that localized regions in the cardiac monolayer can be stimulated enabling us to initiate signal propagation from a precise location. Furthermore, we demonstrate that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network. This approach opens new applications in manipulating and visualizing the electrical activity in a cardiac syncytium.
Construction of a BMP‐2 gene delivery system for polyetheretherketone bone implant material and its effect on bone formation in vitro
Authors: Qin Shuang , Lu Zhengkuan , Gan Kang , Qiao Chunyan , Li Baosheng , Chen Tianjie , Gao Yunbo , Jiang Lingling , Liu Hong 
Published: April 2022

AbstractPolyetheretherketone (PEEK) has been widely investigated for improving its biological inert to enable it to achieve stronger osteogenic capability and to be a promising material in implant fields. The most important mechanism that makes a successful implantation is osteointegration. Surface modification is an appropriate method to maintain the excellent mechanical properties of PEEK and simultaneously endow PEEK certain biological characters. In this work, we attempted to shape the nano‐topography of PEEK surface by nitrogen low‐temperature plasma and polydopamine coating on the surface as a secondary reaction platform to bond the aminated poly (lactic‐co‐glycolic acid) (PLGA) microspheres encapsulating the BMP‐2 gene for enhancing the biological activity. Scanning electron microscope, atomic force microscopy, X‐ray photoelectron spectroscopy and water contact angle (CA) measurements were applied to characterize the surface of modified or untreated PEEK. Surface characterization showed that the modification was successfully performed on PEEK including a rougher and more hydrophilic surface with nanotopographic features. The influence on cell adhesion, proliferation and differentiation was evaluated by culturing of rat bone marrow mesenchymal stem cells on different modified PEEK substrates in vitro. The biological results indicated that the low‐temperature plasma treatment and PDA‐coating on PEEK significantly promoted cell adhesion and proliferation. And the osteogenic differentiation was effectively improved by BMP‐2 gene releasing from PLGA‐NH2 microspheres. The results showed that this novel biological surface modification endowed PEEK with outstanding bioactivity and osteogenic ability, providing a theoretical basis for application in the field of implantation.
A functional corona around extracellular vesicles enhances angiogenesis, skin regeneration and immunomodulation
Authors: Wolf Martin , Poupardin Rodolphe W. , Ebner‐Peking Patricia , Andrade André Cronemberger , Blöchl Constantin , Obermayer Astrid , Gomes Fausto Gueths , Vari Balazs , Maeding Nicole , Eminger Essi , Binder Heide‐Marie , Raninger Anna M. , Hochmann Sarah , Brachtl Gabriele , Spittler Andreas , Heuser Thomas , Ofir Racheli , Huber Christian G. , Aberman Zami , Schallmoser Katharina , Volk Hans‐Dieter , Strunk Dirk 
Published: April 2022

AbstractNanoparticles can acquire a plasma protein corona defining their biological identity. Corona functions were previously considered for cell‐derived extracellular vesicles (EVs). Here we demonstrate that nano‐sized EVs from therapy‐grade human placental‐expanded (PLX) stromal cells are surrounded by an imageable and functional protein corona when enriched with permissive technology. Scalable EV separation from cell‐secreted soluble factors via tangential flow‐filtration (TFF) and subtractive tandem mass‐tag (TMT) proteomics revealed significant enrichment of predominantly immunomodulatory and proangiogenic proteins. Western blot, calcein‐based flow cytometry, super‐resolution and electron microscopy verified EV identity. PLX‐EVs partly protected corona proteins from protease digestion. EVs significantly ameliorated human skin regeneration and angiogenesis in vivo, induced differential signalling in immune cells, and dose‐dependently inhibited T cell proliferation in vitro. Corona removal by size‐exclusion or ultracentrifugation abrogated angiogenesis. Re‐establishing an artificial corona by cloaking EVs with fluorescent albumin as a model protein or defined proangiogenic factors was depicted by super‐resolution microscopy, electron microscopy and zeta‐potential shift, and served as a proof‐of‐concept. Understanding EV corona formation will improve rational EV‐inspired nano‐therapy design.
Use of quality‐of‐life instruments for people living with HIV: a global systematic review and meta‐analysis
Authors: Zhang Ying , He Christine , Peasgood Tessa , Hulse Emily S. G. , Fairley Christopher K. , Brown Graham , Ofori‐Asenso Richard , Ong Jason J. 
Published: April 2022

AbstractIntroductionDue to the effectiveness of combined antiretroviral therapy and its growing availability worldwide, most people living with HIV (PLHIV) have a near‐normal life expectancy. However, PLHIV continue to face various health and social challenges that severely impact their health‐related quality‐of‐life (HRQoL). The UNAIDS Global AIDS Strategy discusses the need to optimize quality‐of‐life, but no guidance was given regarding which instruments were appropriate measures of HRQoL. This study aimed to review and assess the use of HRQoL instruments for PLHIV.MethodsWe conducted a global systematic review and meta‐analysis, searching five databases for studies published between January 2010 and February 2021 that assessed HRQoL among PLHIV aged 16 years and over. Multivariable regression analyses were performed to identify factors associated with the choice of HRQoL instruments. We examined the domains covered by each instrument. Random‐effects meta‐analysis was conducted to explore the average completion rates of HRQoL instruments.Results and discussionFrom 714 publications, we identified 65 different HRQoL instruments. The most commonly used instruments were the World Health Organization Quality‐of‐Life‐ HIV Bref (WHOQOL‐HIV BREF)—19%, Medical Outcome Survey‐HIV (MOS‐HIV)—17%, Short Form‐36 (SF‐36)—12%, European Quality‐of‐Life Instrument‐5 Dimension (EQ‐5D)—10%, World Health Organization Quality‐of‐Life Bref (WHOQOL BREF)—8%, Short Form‐12 (SF‐12)—7% and HIV/AIDS Targeted Quality‐of‐Life (HAT‐QOL)—6%. There were greater odds of using HIV‐specific instruments for middle‐ and low‐income countries (than high‐income countries), studies in the Americas and Europe (than Africa) and target population of PLHIV only (than both PLHIV and people without HIV). Domains unique to the HIV‐specific instruments were worries about death, stigma and HIV disclosure. There were no significant differences in completion rates between different HRQoL instruments. The overall pooled completion rate was 95.9% (95% CI: 94.7−97.0, I2 = 99.2%, p < 0.01); some heterogeneity was explained by country‐income level and study type.ConclusionsA wide range of instruments have been used to assess HRQoL in PLHIV, and the choice of instrument might be based on their different characteristics and reason for application. Although completion rates were high, future studies should explore the feasibility of implementing these instruments and the appropriateness of domains covered by each instrument.
Synergistic impacts of westerlies and monsoon on interdecadal variations of late spring precipitation over the southeastern extension of the Tibetan Plateau
Authors: Wang Jing , Liu Yanju , Song Chengyu , Ding Yihui , Li Qiaoping , Wu Ping , Xu Ying , Xu Xiangde 
Published: April 2022

AbstractBased on multiple long‐term observational and reanalysis datasets, this study investigated the characteristics and physical mechanisms of the interdecadal variations in late spring (i.e., May) precipitation (LSP) over the southeastern extension of the Tibetan Plateau (SETP) since 1900. It was revealed that by and large, LSP over the SETP experienced interdecadal decrease during the period preceding 1927, 1962–1988, and 2004 onwards, but saw an increase during the periods of 1928–1961 and 1989–2003. The atmospheric circulations responsible for interdecadal variations in LSP over the SETP were also analysed. These analyses identified significant synergistic impacts of decreased mid‐latitude upstream westerlies and increased low‐latitude monsoonal southerlies over the Central North Bay of Bengal (CNBOB) on interdecadal variations in precipitation, suggesting striking interactions between extratropical eastward cold air and tropical northward warm/humid air. Further observational and modelling evidence suggested that Atlantic Multidecadal Oscillation (AMO) was likely to be a salient oceanic driver for the interdecadal synergy between upstream westerlies and CNBOB monsoonal southerlies. The elevated sea surface temperature anomalies associated with the warm phase of the AMO could spark favourable local atmospheric anomalies, forcing an upper‐tropospheric, planetary‐scale teleconnection emanating from the east of the North Atlantic sector, which may serve as an effective bridge linking the remote AMO signal and the synergy between westerlies and monsoonal southerlies around the SETP on interdecadal timescales. Our findings provided new insights into the understanding of the synergistic roles of westerlies and monsoons in the modulation of interdecadal LSP over the SETP, prior to the peak Asian summer monsoon season.