bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of SCoT Marker to Discriminate Genotypes and Synthetic Cultivars of Fennel (Foeniculum Vulgare Mill.)
Authors: Akbari Azam , Darbandi Ali Izadi , Ramshini Hosein , Bahmani Keivan 
Published: January 2022

Abstract
Fennel is a member of the Umbelliferae family and one of the most important and commonly used medicinal herbs. Because plant cultivar registration and protection is now confined to a small number of morphological features, the adoption of molecular approaches as complementing tools is unavoidable. In this investigation, the researchers employed ten start codon targeted (SCoT) markers to identify and distinctiveness three synthetic fennel cultivars and eight parental ecotypes. Ten SCoT primers obtained a total of 54 amplified fragments, of which 44 were polymorphic. SC14 and SC2 primers with 9 bands had the most bands, whereas SC17 primers with 5 bands had the least bands. SC29, SC31, and SC2 primers have the greatest polymorphic information content (PIC), Resolving Power (RP), and Marking Index (MI). The genetic similarity of the genotypes analyzed using the Jaccard similarity coefficient varied from 0.31 to 0.76, with an average similarity of 0.54. Genotypes were distinct from one another and split into five categories using cluster analysis, the results of primary coordinate analysis (PCoA) corroborated these findings. These markers proved to be valuable tools for identifying and distinctiveness fennel cultivars due to their good separation of cultivars and independence from environmental effects. As a result, the markers utilized in this research are appropriate for distinction fennel cultivars.
Barriers to Third Mission: Organizational and Individual Antecedents
Authors: Naranjo-Africano Gloria , Vega-Jurado Jaider , Manjarres-Henríquez Liney 
Published: January 2022

Abstract
Barriers limit the development of relations between the university and its environment within the framework of the Third Mission. Although it is widely understood that barriers to successful collaboration and knowledge sharing between universities and other social agents exist, few studies have attempted to measure and map these perceived barriers or investigate what can mitigate them. In this context, this work aims to identify the factors affecting the barriers perceived by academics in their relationship with the environment. The study was based on data derived from a survey applied to a sample of academics categorized in the Sistema Nacional de Ciencia, Tecnología e Innovación of Colombia and related to six higher education institutions located in the Colombian Caribbean region. To analyze the data obtained, binary logistic regression models were established. The results obtained suggest that in addition to the orientation of the research activity, the perception that academics have about the existence of processes or support services for the university’s relationship with its environment is important to reduce the barriers perceived during the relationship. The foregoing implies that the institutions must progress with respect to the institutionalization of the third mission and in strengthening the functions of promotion, counseling, and support as well as promotion structures and, thus, being able to mitigate the barriers perceived in the process.
Establishing the Taxa, Functional profile, and in-silico Ayurvedic Remedy of Microbiota implicated in West Nile Fever
Authors: Bagchi Preenon , Jovita Jemy Ratna , IA Shylesh Murthy , M Anuradha 
Published: January 2022

Abstract
West Nile fever causing microbiome is taken in this work. Culex nigripalpus mosquito is the causative factor for West Nile Virus. Using Metatranscriptomic sequencing, identified the taxa and functional profile of the microbiome is identified. Again, the receptor genes involved in West Nile fever is taken and using computer aided drug design, the novel ligands from Ayurvedic medicinal plants Ginkgo biloba, Uncaria tomentosa, Lycoris radiate and Glycyrrhiza glabra. Further, in-vitro and in-vivo studies can be done on the selected ligands to prove their efficiency as drugs for the disease
Amylolytic Enzyme in Rehydrated Corn and Sorghum Grain Silage in the Diet of Ears
Authors: Silva Janaina Tayna , De Oliveira Euclides Reuter , Gandra Jefferson Rodrigues , Neves Nathálie Ferreira , Gabriel Andrea Maria de Araújo , Monção Flávio Pinto , Okadad Elaine Sayuri Miyagi , andrade wagner da paz , Fernandes Alexandre Rodrigues Mendes , Peixoto Eduardo Lucas Terra 
Published: January 2022

Abstract
Aimed of this work was to evaluate rehydrated corn and sorghum grains silages, with and without α-AMYLASE, on fermentation profile, nutritional value, digestion and metabolism on diets for sheep. Two experiments were conducted. In the first experiment 28 silos were divided into: 1- RSGS (rehydrated sorghum grain silage); 2- RSGS+A (rehydrated sorghum grain silage + amylase,); 3- RCGS (rehydrated corn grain silage); 4 RCGS+A (rehydrated corn kernel silage + amylase). In experiment II, 12 lambs were distributed in: RSGS; RSGS+A; RCGS; RCGS+A. In experiment I, there was an effect of grain x enzyme interaction for GL MN. The enzyme reduced the NFC content. In experiment II intake of DM kg/day was not affected by the starch content in the diet, with an average of 1.2 kg/day meaning that the type of grain and the enzyme had no influence on the intake of animals. There was an interaction for the intake of starch (kg/day), blood urea and N-NH 3. Lambs fed with RCGS+A had a higher concentration of ruminal ammonia. The use of enzymes improved the fermentation profile of the silages. RSGS can replace RCGS in sheep feed without modifying nutrient intake and digestibility.
Pregnancy Outcomes Among Women Affected With Thalassemia Traits
Authors: Ruangvutilert Pornpimol , Phatihattakorn Chayawat , Yaiyiam Chutima , Panchalee Tachjaree 
Published: January 2022

Abstract
ObjectiveTo compare the maternal and perinatal outcomes between a group of pregnant women diagnosed with thalassemia traits and normal controls.Study designA retrospective cohort study was conducted on singleton pregnant women affected and unaffected by thalassemia traits who attended an antenatal care clinic and delivered in Siriraj Hospital. Thalassemia status for all subjects was diagnosed by hemoglobin typing and/or DNA analysis. Patient charts were reviewed from January 2007 to December 2018. The control participants were randomly selected from the same period, with a control-case ratio of around 1:1.ResultsOverall, 1288 women with thalassemia traits (348 with α thal-1 trait, 424 with β thal trait and 516 with HbE trait) and 1305 women in the control group were recruited. Baseline characteristics of both groups were similar, with the exception that the hematocrit level in the first trimester in the thalassemia trait group was significantly lower than that in the control group (34.8±3.4% VS 36.9±3.0%; p < 0.001). The prevalence of pregnancy-induced hypertension (PIH) was higher in the thalassemia trait group, at 6.9% VS 4.7% in the control group; p = 0.018. When subgroups were analyzed between each thalassemia trait, the number of maternal anemias in the first and third trimester were higher for all thalassemia traits compared to the normal group. The β thal and HbE traits increased the risk of PIH, with a relative risk (RR) = 1.67 and 1.66, respectively. Additionally, the rate of neonates with a low Apgar score at 1 minute was significantly higher in the HbE trait; RR = 2.06.ConclusionsThalassemia traits minimally but significantly increase the risk of hypertensive disorders and low Apgar score in pregnancy. In addition, physiological changes during pregnancy may worsen the severity of anemia in the pregnant women with thalassemia traits.
Exploratory study on interaction between bacteria and fungi from mulberry to intestine of Bombyx mori
Authors: Li Chengxu , Xu Shuo , Xiang Chunjie , Xu Shixia , Zhou Qihai , zhang junfeng 
Published: January 2022

Abstract
Many kinds of antibiotics in the environments deeply affect the microbial composition, but their effect on the interactions among the host microbiota is still poorly understood. This study used Bombyx mori as the model organism by feeding the antibiotics-treated mulberry leaf, preliminarily explored the effects of antibiotic exposure on the interaction of bacteria and fungi in the worm’s intestine. Our results showed that the elimination of fungi significantly reduced the bacterial richness and diversity in the worm’s intestine after exposure of anti-fungal amphotericin B, while the elimination of bacteria dramatically increased the richness and diversity of fungi after exposure of anti-bacterial ampicillin-streptomycin. The results revealed the intestinal bacteria and fungi of Bombyx mori were markedly shaped by the mulberry leaf flora. Anti-bacterial antibiotics enhanced the correlation between the host gut fungi and the diet-deriving fungi, while anti-fungal antibiotics weakened the correlation between the host gut bacteria and the diet-deriving bacteria. The data preliminarily established a simple model to explore the effect of antibiotics on the host microbiota interactions, and provided a way for further study on the environmental factors of intestinal bacteria-fungi interaction.
Deep-potential enabled multiscale simulation of gallium nitride devices on boron arsenide cooling substrates
Authors: Wu Jing , Zhou E , Huang An , Zhang Hongbin , Hu Ming , Qin Guangzhao 
Published: January 2022

Abstract
High-efficient heat dissipation plays critical role for high-power-density electronics. Experimental synthesis of ultrahigh thermal conductivity boron arsenide (BAs, 1300 W m−1K−1) cooling substrates into the wide-bandgap semiconductor of gallium nitride (GaN) devices has been realized [Nature Electronics 4, 416-423 (2021)]. However, the lack of systematic analysis on the heat transfer across the BAs-GaN interface hampers the practical applications. In this study, by constructing the accurate and high-efficient machine learning interatomic potentials, we performed multiscale simulations of the BAs-GaN heterostructures. Ultrahigh interfacial thermal conductance (ITC) of 265 MW m−2K−1 is achieved, which lies in the well-matched lattice vibrations of BAs and GaN. Moreover, the competition between grain size and boundary resistance was revealed with size increasing from 1 nm to 100 µm. Such deep-potential equipped multiscale simulations not only promote the practical applications of BAs cooling substrates in electronics, but also offer new approach for designing advanced thermal management systems.
Comparative Meta-analysis of Adipose Tissue Transcriptomics Data in PCOS Patients and Healthy Controls Women
Authors: Saedi Saman , Panahi Reza , Orak Najmeh , Shirazi Mohammad Reza Jafarzadeh 
Published: January 2022

Abstract
Background Women with polycystic ovary syndrome (PCOS) seem to have disturbances in lipid metabolism in subcutaneous adipose tissue. Nevertheless, gene expression in subcutaneous adipose tissue of PCOS women and its relation to other disturbances have been fragmentarily investigated. Methods and results We utilized microarray data to identify the most important up and down-regulated candidate genes in adipose tissue of PCOS women in contrast to healthy women using the meta-analysis technique. Microarray data produced from three independent experiments (n=3) conducted on adipose tissue in women with PCOS were retrieved from ArrayExpress. Then, the datasets were merged using the metaSeq package in Rstudio and differentially expressed genes (DEGs) in studies were selected. The integrative bioinformatics analyses of candidates were performed by gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and Protein-protein interaction (PPI) network construction. Twelve up-regulated genes and twelve down-regulated genes were identified and assessed as the most important DEGs. These the most important DEGs that were annotated by KEGG analysis were mainly involved in PI3K-Akt signaling pathway, pathways in cancer, breast cancer, metabolic pathways, ovarian steroidogenesis, and insulin secretion. In the PPI networks, PRDM10, FGFR2, IGF1R, and FLT1 were the key nodes in the up-regulated networks, while the NDUFAB1 and NME2 proteins were key in the down-regulated networks. Conclusion Overall, these findings provide a comprehensive system biology based insight into the gene expression in subcutaneous adipose tissue of PCOS women and its relation to other disturbances.
A Retrospective Analysis on the Clinical Efficiency of Dual Trigger During Vitro Fertilization and Embryo Transfer (IVF-ET) Treatment for Women with Diminished Ovarian Reserve
Authors: Zhang Ling , Wu Li-mei , Huang Qiong-xiao , Ji Meng-xia , Li Shi-shi , Zhang Lin , Jin Zhen , Zhou Yi-er , Shu Chong-yi , Shu Jing , Xu Wei-hai 
Published: January 2022

Abstract
Background: Due to poor number and quality of oocytes, the reproductive prognosis of diminished ovarian reserve (DOR) patients is obstinately disapproving. This study will compare the effects of double trigger and hCG trigger on the culture outcome and implantation outcome during the IVF treatment for DOR patients. Methods: a total of 857 controlled ovarian stimulation (COS) cycles and 366 frozen embryo transfer (FET) cycles were included in this study. The difference in retrieval cancellation, oocyte retrieval, number of oocytes, maturation oocytes, fertilization, embryos were evaluated between the two groups in the COS dataset, while implantation, clinical pregnancy, ongoing pregnancy, and spontaneous abortion were analyzed based on FET dataset. To robustly evaluate the marginal effect of the study factor as well as other covariates, a cluster-weighted GEE model was exploited to fit the uniqueness of IVF-ET data.Results: Neither the retrieval outcome, nor cultural or implantation outcomes were improved after dual-trigger. The OR values were 1.08 (95% CI: 0.41 ~2.78) for retrieval cancellation, 1.33 (95% CI: 0.89 ~2.00) for oocyte harvest, 0.99 (95%: 0.97 ~1.02) for oocyte maturation, 1.03 (95%CI: 0.94 ~1.12) for fertilization, 1.04 (95%CI: 0.94 ~1.15) for viable embryo, and 1.03 (95%CI: 0.88 ~1.19) for top quality embryo. As to the transfer outcome, no difference was identified between the two groups, with the ORs 0.90 (95%CI: 0.62 ~1.30) for implantation and 0.97 (95%CI: 0.56 ~1.69) for clinical pregnancy. This insignificance remained unchanged after adjusting for the covariates such as age, BMI, infertility duration, etc.Conclusions: Dual triggering the final oocyte maturation combined of GnRH-a and standard dose of hCG cannot significantly improve the retrieval, cultural or implantation outcomes of IVF-ET treatments in women with DOR.
A quantitative analysis of the relationship between absorbed and applied phosphorous and their contribution to seed production in soybeans under various applied nitrogen conditions
Authors: Kakiuchi Jin , Kobata Tohru 
Published: January 2022

Abstract
Aims

This study aimed to model the relationship between assimilated (PA) and applied (PF) phosphorus under diverse nitrogen applications (NF), and to determine the contribution of P to N assimilation (NA) and seed production in soybeans [Glycine max (L.) Merr.], as this has not been done before.
Methods

Over three years, pot experiments were conducted using nodulated [Lee(+)] and non-nodulated isoline [Lee(–)] soybeans under multiple levels of basal-dressed PF and top-dressed NF from R2 to R7.
Results

PA in each NF was represented by a function of PF as a straight line with a positive slope and an intercept (PS, soil P) connecting with a ceiling line of constant height. The slope [SLOPE = (PA – PS) / PF], which represented the efficiency of PF usage for PA, asymptotically increased with NF. As a result, PA was represented by a single function of PF × SLOPE, which consisted of slope = 1 and a ceiling across different NF rates. Seed production each year was explained by NA regardless of PA for both nodulation types, although responses of seed yield to PA in Lee(–) differed due to NA. PA asymptotically increased the maximum NA and N fixation by nodulation which were approximated by the N quantitative model.
Conclusion

PA can be represented as a function of PF by excluding P retained in soils, which is influenced by NF. Accordingly, PA effects seed production via NA behavior determined by fertilizer, soil and fixed N.
CXCR7 agonist TC14012 improves angiogenic function of endothelial progenitor cells via activating Akt/eNOS pathway and promotes ischemic angiogenesis in diabetic limb ischemia
Authors: Wang Kai , Sun Shiyue , Zhang Guigui , Lu Zixian , Chen Hui , Fan Xia , Gu Chunjie , Pan Xiaohong , Lin Qian , Chen Oscar , Cai Lu , Dai Xiaozhen , Yan Xiaoqing , Tan Yi 
Published: January 2022

Abstract
Purpose Endothelial progenitor cells (EPCs) play a critical role in repairing damaged vessels and triggering ischemic angiogenesis, but their number is reduced and function is impaired under diabetic conditions. Improving EPC function has been considered as a promising strategy to ameliorate diabetic vascular complications. In present study, we want to investigate whether and how CXCR7 agonist TC14012 promotes the angiogenic function of diabetic EPCs. Methods High glucose (HG) treatment was used to mimic the hyperglycemia in diabetes. Tube formation, cell scratch recovery, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were used to evaluate the angiogenic capability, cell migration and apoptosis of EPCs, respectively. Hind limb ischemia (HLI) model was used to appraise the ability of TC14012 in promoting diabetic ischemic angiogenesis in vivo. Results HG treatment impaired EPC tube formation and migration, and induced EPC apoptosis and oxidative damage, while TC14012 rescued tube formation and migration, and prevented HG-induced apoptosis and oxidative damage of EPCs. Furthermore, these beneficial effects of TC14012 on EPCs were attenuated by specific siRNAs against CXCR7, validating that CXCR7 is a functional target of TC14012 in EPCs. Mechanistic studies demonstrated that HG treatment reduced CXCR7 expression in EPCs, impaired Akt and endothelial nitric oxide synthase (eNOS) phosphorylation and nitric oxide (NO) production; similarly, these signal impairments in HG-exposed EPCs could be rescued by TC14012. However, the protective effects of TC14012 on Akt and eNOS phosphorylation and NO production were almost completely abolished by siRNAs against CXCR7 or Akt specific inhibitor Wortmannin. More importantly, in vivo study showed that TC14012 administration enhanced blood perfusion recovery and angiogenesis in the ischemic hind limb and increased the EPC number in circulating of db/db mice, demonstrating the capability of TC14012 in promoting EPC mobilization and ischemia angiogenic function.Conclusion TC14012 can prevent EPCs from HG-induced dysfunction and apoptosis, improve eNOS activity and NO production via CXCR7/Akt signal pathway, and promote EPC mobilization and diabetic ischemia angiogenesis.
Efflux Dynamics of the Anti-Epileptic, Levetiracetam, Through the P-Glycoprotein Channel Revealed by Advanced Comparative Molecular Simulations
Authors: Behmard Esmaeil , Barzegari Ebrahim , Najafipour Sohrab , Kouhpayeh Amin , Ghasemi Younes , Asadi-Pooya Ali A. 
Published: January 2022

Abstract
Understanding the precise mechanistic details of the possible binding and transport of antiepileptic drugs through the efflux pump, P-glycoprotein (P-gp), is essential to find strategies for the treatment of epileptic patients resistant to anti-seizure medicines. In the present work, conventional molecular dynamics, binding free energy calculations, steered molecular dynamics and umbrella sampling were applied to study the interactions of levetiracetam and brivaracetam with P-gp and their possible egress path from the binding site. Comparative results for the control drugs, zosuquidar and verapamil, confirmed their established P-gp inhibitory activity. Brivaracetam, a non-substrate of P-gp, demonstrated stronger static and dynamic interactions with the exporter protein, than levetiracetam. The potential of mean force calculations indicated that the energy barriers through the ligand export were the lowest for levetiracetam, suggesting the drug as a P-gp substrate with facile passage along the transporter channel. Our findings also stressed the contribution of nonpolar interactions with P-gp channel lining as well as with membrane lipid molecules to hampering the antiepileptic drug efflux by the transmembrane exporter. Appropriate structural engineering of the anti-seizure medications is thus recommended to address the drug-resistant epilepsy.
Weather Factors in Different Growing Periods Determine Inter-annual Change in Growth of Four Sphagnum Species: Evidence From an Eight-year Study
Authors: Koronatova Natalia G. , Kosykh Natalia P. , Saib Ekaterina A. , Stepanova Vera A. , Vishnyakova Evgenia K. , Granath Gustaf 
Published: January 2022

Abstract
The growth of peat mosses is crucial for the functioning and carbon uptake and storage in high latitude peatlands. Nevertheless, a little is known on the response of Sphagnum functional traits and primary production to weather fluctuations. Here we investigated if weather variables affect Sphagnum growth differently across the season. An eight-year study was carried out in a mire complex in the southern taiga of Western Siberia. We collected weather data, and data on functional traits (length increment, increment weight, capitulum numerical density and capitulum weight) and assessed primary productivity of four Sphagnum species growing in raised bogs and poor fens. Overall, growing season averages of weather variables were often as good predictors of Sphagnum functional traits and productivity as early or late seasonal averages, but not necessarily for the same variables. The most important factors were those related moisture conditions. The more sensitive traits to weather fluctuation were length increment (for S. magellanicum, S. angustifolium, S. fallax) and numerical density (for S. fuscum). Primary productivity of S. fuscum and S. magellanicum were the highest under warm and wet conditions with S. fuscum being less sensitive to the weather; productivity of S. angustifolium increased under cool and wet conditions; and productivity of S. fallax was the highest under dry weather. Diversity in interspecific response to weather fluctuations may result in high peatland resistance towards environmental variability, and thereby maintaining a stable Sphagnum productivity in time across the mire complex.
Indirect All-Quadrilateral Meshing Based on Bipartite Topological Labeling.
Authors: Georgiadis Christos , Reberol Maxence , Remacle Jean-François 
Published: January 2022

Abstract
Quadrilateral meshes offer certain advantages compared to triangular ones, such as reduced number of elements and alignment with problem-specific directions. We present a pipeline for the generation of quadrilateral meshes on complex geometries. It is based on two key components: robust surface meshing and efficient indirect conversion of a triangular mesh to an all-quad one. The input is a valid geometric surface mesh, i.e., a triangulation that accurately represents the geometry of the model. A right-angled triangular surface mesh is initially created by continuously modifying the input mesh while always preserving its topological validity. The main advantages of our local mesh modification based approach are to (i) allow the generation of meshes that are globally aligned with a given direction field and (ii) to reliably handle non-manifold feature edges (in multi-volume models) and small features. The final quadrilateral mesh is obtained by merging pairs of triangles into quadrilaterals. We develop a novel bipartite labeling scheme in order to identify and correct inconsistent pairings. The procedure is based on local operations and is much more efficient than previous global strategies for tri-to-quad conversion. The whole pipeline is tested on a large number of models with diverse characteristics.