bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid Detection of SARS-CoV-2 Variants by Molecular Clamping Technology Based RT-qPCR
Authors: Shen Shuo , Fu Andrew , Jamba Maidar , Li Jonathan , Cui Zhen , Pastor Larry , Cataldi Daniel , Sun Qing , Pathakamuri Joseph , Kuebler Daniel , Rohall Michael , Krohn Madison , Kissinger Daniel , Neves Jocelyn , Archibeque Isaac , Powell Mike , Zhang Aiguo , Lu Chuanyi , Sha Michael 
Published: August 2022

Abstract

Given the challenges that fast-changing SARS-CoV-2 variants have caused in terms of rapid spread and reduced vaccine efficacy, a rapid and cost-effective assay that can detect new and emerging variants is greatly needed worldwide. We have successfully applied the xenonucleic acid-based molecular-clamping technology to develop a multiplex RT-qPCR assay for SARS-CoV-2 multivariant detection. The assay was tested on 649 nasopharyngeal swab samples that were collected from California and Ohio. The assay was able to correctly identify all 36 Delta variant samples as it accurately detected D614G, T478K and L452R mutations. In addition, the assay was able to correctly identify all 34 Omicron samples by detecting K417N, T478K, N501Y and D614G mutations. This technique reliably detects a variety of variants and has an analytical sensitivity of 100 copies/mL. In conclusion, this novel assay can serve as a rapid and cost-effective tool to facilitate large-scale detection of SARS-CoV-2 variants.
HealthBlock: A Modular Framework for a Collaborative Sharing of Electronic Health Records Based on Blockchain
Authors: abdelgalil LEINA Nazar , Mejri Mohamed 
Published: August 2022

Abstract
Electronic Health Records (EHRs) play an important role in our life. However, most of the time, they are scattered and saved on different databases belonging to distinct institutions (hospitals, laboratories, clinics, etc.) geographically distributed across one or many countries. Due to this decentralization and the heterogeneity of the different involved systems, the medical staff are facing difficulties in correctly collaborating by sharing, protecting, and tracking their patient’s electronic health-record history to provide them the best cares. Also,

patients have no control on their private EHRs. Blockchain has many promising futures useful for the healthcare domain because it provides a better solution for sharing data while preserving the integrity, the interoperability, the availability, and even the privacy than classical client-server architectures used to manage EHRS.

This paper proposes a modular framework called HealthBlock for a collaborative sharing EHRs and their privacy-preserving with a granular access control. Different technologies have been combined to access this goal. the InterPlanetary File System (IPFS) technology stores and shares patients’ EHRs in distributed Off-Chain storage and ensures the record’s immutability; the Hyper-ledger Indy gives patients full control over their EHRs and the Hyper-ledger Fabric store the patient access control policy and delegations.
New evidence on the quagga mussel spread south to the Alps from an eDNA approach
Authors: Capelli Camilla , Mauri Federica , Pianta Elisa , Rotta Federica , Lepori Fabio 
Published: August 2022

Abstract
The dreissenid quagga mussel, Dreissena rostriformis bugensis, is an invasive species native to the Black Sea region that has recently spread to Western Europe. This organism has recently colonized Swiss northern perialpine lakes, causing major changes to aquatic ecosystems and impacts on human activities. Due to its competitiveness, the quagga mussel takes advantage of human mediated transport to reach new environments. Therefore, the management of quagga mussel should seek to prevent further spread and minimize its negative impact. With this aim, the early detection by environmental DNA (eDNA) methods is increasingly applied in freshwater systems. Here, an approach based on species-specific primer targeting the quagga mussel from eDNA was applied to Lake Lugano and Lake Maggiore, two large perialpine lakes at risk of invasion due international tourism. Our results are the first to show that the quagga mussel has spread south of the Alps and suggest that in both the lakes the colonization is in the early stages. Additionally, they indicate that the eDNA approach is a powerful tool for the early detection of quagga mussel invasions, and may be applied to reduce the resulting ecological and economic impacts.
Neuromedin U-deficient Rats do not Lose Body Weight or Food Intake
Authors: Yokogi Kyoka , Goto Yuki , Otsuka Mai , Ojima Fumiya , Kobayashi Tomoe , Tsuchiba Yukina , Takeuchi Yu , Namba Masumi , Kohno Mayumi , Tetsuka Minami , Takeuchi Sakae , Matsuyama Makoto , Aizawa Sayaka 
Published: August 2022

Abstract
Studies in genetically modified mice establish that essential roles of endogenous neuromedin U (NMU) are anorexigenic function and metabolic regulation, indicating that NMU is expected to be a potential target for anti-obesity agents. However, in central administration experiments in rats, inconsistent results have been obtained, and the essential role of NMU energy metabolism in rats remain unclear. This study aims to elucidate the role of endogenous NMU in rats. We generated NMU knockout (KO) rats that unexpectedly showed no difference in body weight, adiposity, circulating metabolic markers, body temperature, locomotor activity, and food consumption in both normal and high fat chow feeding. Furthermore, unlike reported in mice, expressions of Nmu and NMU receptor type 2 (Nmur2) mRNA were hardly detectable in the rat hypothalamic nuclei regulating feeding and energy metabolism, including the arcuate nucleus and paraventricular nucleus, while Nmu was expressed in pars tuberalis and Nmur2 was expressed in the ependymal cell layer of the third ventricle. These results indicate that the species–specific expression pattern of Nmu and Nmur2 may allow NMU to have distinct functions across species, and that endogenous NMU does not function as an anorexigenic hormone in rats.
Association between serum homocysteine and sarcopenia in the Chinese elderly population: A cross-sectional study
Authors: Lu Bing , Shen Lingyu , Zhu Haiqiong , Xi Ling , Wang Wei , Ouyang Xiaojun 
Published: August 2022

Abstract
Objection: Hyperhomocysteine (HHcy) is considered to increase the risk of Sarcopenia (SA) and remains controversial. In this study, we aimed to evaluate the association between homocysteine (Hcy) and SA in the Chinese elderly population. Methods: A cross-sectional study of 441 elderly patients, who underwent the measurements of muscle mass, muscle strength, physical performance, and serum Hcy from 2017 to 2021 at Geriatric Hospital of Nanjing Medical University, was carried out to assess the relationship between SA and Hcy. All baseline characteristics and laboratory examination of subjects were collected and analyzed by specific personnel. The strength of association between Hcy and the risk of SA was analyzed by multivariate logistic regression to calculate the odds ratios (ORs) and 95% confidence interval (CI). Results: Among the 441 subjects, 161 (36.51%) cases were diagnosed as SA, and 343 of them were found with HHcy. A significant association was detected between SA and serum Hcy per 1μmol/L increase(adjusted OR = 1.068, 95% CI = 1.021–1.117, p =0.004). In a gender-based subgroup analysis, the subjects with HHcy had an increased risk of SA in female group (adjusted OR 1.098, 95%CI 1.009–1.197, p = 0.036), but not in males. Furthermore, HHcy had a significantly positively associated with SA in the very elderly whose age was more than or equal to 85 (adjusted OR = 1.112, 95% CI = 1.037–1.193, P = 0.003).Conclusions: Hcy has a positive association with SA in Chinese urban population, especially in the elderly females. That suggests a hypothesis that the downward adjustment of Hcy might decrease the risk of SA.
A Hybrid Particle Swarm Optimisation-Genetic Algorithm for Multi-objective Reservoir Ecological Dispatching
Authors: Wu Xu , Shen Xiaojing , Wei Chuanjiang , Xie Xinmin , Li Jianshe 
Published: August 2022

Abstract
Reservoir ecological dispatching is a complex system problem with multi-objective, multiple-criteria and multiple-phase. This study establishes a multi-objective ecological dispatching model in Changchun city of Yinma River Basin based on the water demand of social economic development, river ecology, and the constraint of reservoir characteristic parameters. Taking the advantages of particle swarm optimisation (PSO) and genetic algorithm (GA), a PSO-GA hybrid algorithm is proposed and applied to solve the schemes of ecological dispatching model considering different ecological flow requirements. The annual mean scheduling results show that the three scheduling schemes basically achieve the objectives of river ecological base flow scheduling. While for the ecological suitable flow, the guarantee rate of Dehui section in RGOS1/2/3 scheme is 79.79%/87.95%/96.08%, and that of Nongan section is 82.98%/90.85%/96.45%. The scheduling results of typical years show that the water security situation in the study area is not optimistic, but the river ecological environment can be greatly improved by reservoir ecological dispatching. Finally, the high quality and stable search performance of the hybrid PSO-GA proposed in this study is verified by comparing with other algorithms. The mean value and standard of the objective function of the 20 simulation results calculated by PSO-GA are 97.75% and 0.11 respectively, which are better than other algorithms.
Chemical forms of cadmium in soil and its distribution in French marigold sub-cells in response to chelator GLDA
Authors: Li Hongchuan , Kong Deming , Zhang Borui , Kalkhajeh Yusef Kianpoor , Zhao Yingying , Huang Jieying , Hu Hongxiang 
Published: August 2022

Abstract
The use of degradable chelating agents to facilitate phytoextraction is a promising low-cost method for the remediation of heavy metal-contaminated soils. However, there are few studies on how plants and soils respond to the chelating agents. In this study, the responses of French marigold(Tagetes patula L.) and soil cadmium (Cd) to the chelator tetrasodium glutamate (GLDA) was investigated in a 180 d field trial. Five GLDA treatments (0,292.5, 585, 1170, and 2340 kg hm− 2) were carried out in a Cd-contaminated soil (0.47 mg kg− 1) under French marigold plantation. The results showed that the application of GLDA significantly increased the exchangeable Cd and cell wall Cd accumulations by 42.13% and 9.39% (p < 0.05), respectively. Furthermore, increases occurred in soil pH, as well as DOC and DTPA-Cd contents with increasing the total amount of GLDA. The composite application of GLDA (2340 kg hm− 2) with French marigold reduced the total soil Cd content by 7.59% compared with the soil background. Altogether, results of this study suggested that the application of GLDA can effectively activate soil Cd and enhance the capability of French marigold for the remediation of Cd-contaminated soils.
Oncostatin M stimulates prostaglandin D 2 -induced osteoprotegerin and interleukin- 6 synthesis in osteoblasts
Authors: Kuroyanagi Gen , Hioki Tomoyuki , Tachi Junko , Matsushima-Nishiwaki Rie , Iida Hiroki , Kozawa Osamu , Tokuda Haruhiko 
Published: August 2022

Abstract
Oncostatin M produced by osteal macrophages has important roles in fracture healing. Osteoprotegerin (OPG) secreted by osteoblasts, binds to RANK ligand (RANKL) as a decoy receptor, and prevents RANKL from binding to RANK, resulting in the suppression of bone resorption. IL-6 is a pro-inflammatory cytokine and generally regulates bone resorption. However, accumulating evidence suggests that IL-6 plays pivotal roles in bone formation. We previously showed that prostaglandin D2 (PGD2) induces OPG synthesis by activating p38 mitogen-activated protein (MAP) kinase, stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), and p44/p42 MAP kinase in osteoblast-like MC3T3-E1 cells. We also demonstrated that PGD2 stimulates IL-6 synthesis by activating p38 MAP kinase and p44/p42 MAP kinase in MC3T3-E1 cells. In the present study, we investigated whether oncostatin M affects PGD2-stimulated OPG and IL-6 synthesis in MC3T3-E1 cells through the MAP kinase activation. The osteoblast-like MC3T3-E1 cells were treated with oncostatin M and then stimulated with PGD2. OPG and IL-6 synthesis were measured using an OPG and IL-6 enzyme-linked immunosorbent assay kit. OPG and IL-6 mRNA expression levels were evaluated by real-time RT-PCR. The phosphorylation of p38 MAP kinase, SAPK/JNK, and p44/p42 MAP kinase were evaluated by Western blotting. As a result, oncostatin M significantly increased the PGD2-stimulated OPG and IL-6 release. Oncostatin M significantly enhanced mRNA expression levels of OPG and IL-6 induced by PGD2. Regarding the signaling mechanism, oncostatin M did not affect the phosphorylation of p38 MAP kinase, SAPK/JNK, and p44/p42 MAP kinase. Our results suggest that oncostatin M upregulates the PGD2-stimulated OPG and IL-6 synthesis in osteoblasts and therefore affect bone remodeling. However, OPG and IL-6 synthesis are not mediated through p38 MAP kinase, p44/p42 MAP kinase, or SAPK/JNK pathways.
Synthetic torpor protects rats from exposure to accelerated heavy ions
Authors: Puspitasari Anggraeini , Squarcio Fabio , Quartieri Martina , Totis Cristina , Hitrec Timna , Takahashi Akihisa , Yoshida Yukari , Hanamura Kenji , Yako Tomoko , Cerri Matteo , Simoniello Palma , Durante Marco , Tinganelli Walter 
Published: August 2022

Abstract
Hibernation or torpor is considered a possible tool to protect astronauts from the deleterious effects of space radiation that contains high-energy heavy ions. We induced synthetic torpor in rats by injecting adenosine 5′-monophosphate monohydrate (5′-AMP) i.p. and maintaining in low ambient temperature room (+ 16°C) for six hours immediately after total body irradiation (TBI) with accelerated carbon ions (C-ions). The 5′-AMP treatment in combination with low ambient temperature reduced skin temperature and increased survival following 8 Gy C-ion irradiation compared to saline-injected animals. Analysis of the histology of the brain, liver and lungs showed that 5′-AMP treatment following 2 Gy TBI reduced activated microglia, Iba1 positive cells in the brain, apoptotic cells in the liver, and damage to the lungs, suggesting that synthetic torpor spares tissues from energetic ion radiation. The application of 5′-AMP in combination with either hypoxia or low temperature environment for six hours following irradiation of rat retinal pigment epithelial (RPE-J) cells delays DNA repair and suppresses the radiation-induced mitotic catastrophe compared to control cells. We conclude that synthetic torpor protects animals from cosmic ray-simulated radiation and the mechanism involves both hypothermia and hypoxia.
DFT Analysis And In-Vitro Studies Of Isoxazole Derivatives As Potent Antioxidant And Antibacterial Agents Synthesized via One- pot Methodology
Authors: Thakur Ajay , Verma Monika , Setia Parul , Bharti Ruchi , Sharma Renu , Sharma Ajay , Negi Neelam P. , Anand Vivek , Bansal Ravi 
Published: August 2022

Abstract
Isoxazole and its derivatives derived from natural resources are very few. However, they have several applications in pharmaceutical industries, including antimicrobial, antitumor, anticancer, antifungal, and antibacterial activities. As a result, this research aimed to design a novel one-pot green approach to synthesize new oxazole derivatives. The derivatives were further explored for their antibacterial and antioxidant activities together with their DFT analysis. Characterization of newly synthesized moieties was done by IR, 1H NMR, 13C NMR, CHN analysis, & single-crystal X-ray Crystallography. Further, these compounds were examined for their antibacterial potential by using Gentamycin as a standard drug against S. aureus and E. colibacterial strains. The derivatives 4a, 4c, 4d, 4f, 4j, and 4k possessed excellent antibacterial potential against former, while 4c and 5 showed the highest activity against thelater one. The derivatives were also analyzed for their antioxidant activities by using free radical scavenging (ABTS. & DPPH assays), and Total antioxidant capacity (TAC). Here also, 4a, 4d, 4e, 4k, 4l, 4m, and 5 exhibited the most promising results. Finally, the density functional theory (DFT) analysis was achieved by using the B3LYP methodology with a 6-311+G(d,p) basis set to study the electronic structure of molecules and analysis of chemical reactivity descriptors such as hardness (η), Mulliken electronegativity (χ), chemical potential (μ) and electrophilicity (ω). These properties were calculated from the levels of the predicted frontier molecular orbitals and their energy gap.
Characterization of kefir yeasts with antifungal capacity against Aspergillus species
Authors: Moure María Candela , Torrado Roberto Pérez , Garmendia Gabriela , Vero Silvana , Querol Amparo , Alconada Teresa , Peláez Ángela León 
Published: August 2022

Abstract
Kefir is a fermented probiotic drink obtained by placing kefir granules in a suitable substrate. The kefir granules are a consortium of bacteria and yeasts embedded in a exopolysaccharide matrix. The aim of this research was the isolation and identification of yeasts from kefir of different origin, the evaluation of their antifungal capacity against Aspergillus spp. and the characterization of virulence related traits. Using RFLP of ITS1/ITS4 region, D1/D2 region sequencing and RAPD techniques, 20 kefir isolates were identified as Geotrichum candidum, Pichia kudriavzevii, P. membranifaciens, Saccharomyces cerevisiae and Candida ethanolica. Their antifungal capacity was evaluated by their conidia germination reduction, which allowed the selection of eight isolates with high to moderate conidia germination reduction against A. flavus and A. parasiticus. Furthermore, these selected isolates showed growth inhibition on contact in the dual culture assay for both Aspergillus species and 3 of them ‒belonging to S. cerevisiae and P. kudriavzevii species‒ generated volatile organic compounds which significantly affected the growth of both fungi. For the evaluation of virulence related traits, growth at high temperatures, enzymatic activities and the adhesion to Caco-2 cells were analyzed. The isolates did not present more than one positive virulence-related trait simultaneously. In particular, it is important to highlight that the adhesion capacity to the model of intestinal barrier was extremely low for all of them. According to the results obtained, further studies would be of interest for the possible use of these promising yeasts as biocontrol agents against fungi in food.
Linc01060 suppresses ferroptosis by downregulating SPOP-mediated c-Myc degradation in glioma
Authors: Li Jun Jun , Wang Qiang Ping , Jiang Cheng , Wang Min Jie , Tang Nan , Zhou Zi Jie , Zhu Kai , Cao Yu , Hu Guang Fu , Wang Si Qi , Shu Peng Wei , Yuan Hong Liang , Xiong Zhi Yong , Li Jin Song , Ouyang Tao Hui , Liu Hong Ya , Qiu Lie Wang , Wang Xuan , Li Lin , Hu Xue Bin , Zhang Fang Cheng , Zhou Ying Chun , Zhao Hong Yang , Rao Jing , Jiang Xiao Bing 
Published: August 2022

AbstractBackground:Ferroptosis is a type of regulated cell death induced by iron-dependent lipid peroxidation that plays an essential role in the pathogenesis of various diseases, including cancer. Previously, we reported that long non-coding RNA (lncRNA) Linc01060 regulates aggressive phenotype and aerobic glycolysis in glioma cells. However, the precise functions and underlying molecular mechanisms of Linc01060 in ferroptosis remain unknown.Methods:We explored the upstream regulatory mechanism of Linc01060 expression in hypoxic conditions in glioma cells. The expression and prognostic significance of Linc01060 in patients with glioma was analyzed using data from the Cancer Genome Atlas. The role of Linc01060 in regulating ferroptosis in glioma was evaluated bothin vitroandin vivo. Linc01060 expression in clinical glioma specimens was evaluated by fluorescence in situ hybridization, immunofluorescence, and immunohistochemistry.Results:Linc01060 expression was found to be transactivated by hypoxia inducible factor-1 alpha. A strong correlation was found between Linc01060 expression and ferroptosis biomarkers, suggesting that Linc01060 is a negative regulator of ferroptosis. Linc01060 rescued erastin-induced ferroptosis and promoted tumor progression in glioma cells bothin vitroandin vivo. Speckle-type POZ protein (SPOP), an E3 ligase of c-Myc, was identified as the target protein of Linc01060. We found that mechanistically Linc01060 competitively binds to SPOP, downregulating SPOP-mediated c-Myc degradation, and thereby decreasing the sensitivity of glioma cells to ferroptosis.Conclusion:Linc01060 suppresses ferroptosis by modulating the SPOP/c-Myc axis in glioma cells. Our results suggest that targeting Linc01060 is a potential strategy for the treatment of tumors.
Time series prediction of mine pressure in distributed optical fiber monitoring based on ARMA-SVR
Authors: Chai Jing , Wang Mingbo , Wang Anyi , OuYang Yibo 
Published: August 2022

Abstract
In this study, the distributed optical fiber is used to monitor the overburden changes during the coal mining process, and the average frequency shift change degree of optical fiber is introduced as the index to judge the periodic pressure. The ARMA-SVR model composed of auto-regressive moving average (ARMA) and support vector machine regression (SVR) is established to predict the rock pressure behavior through the prediction of the frequency shift change of optical fiber. Firstly, a similar material model is built to obtain the data of the frequency shift of the optical fiber during the excavation of the working face; Then, the frequency shift change sequence of optical fiber is converted into a time series. Finally, a combined model is built. The linear auto-correlation part of the frequency shift change sequence is predicted by ARMA model, and the nonlinear residual part is predicted by SVR model to jointly complete the prediction of the frequency shift change of optical fiber. The experimental results show that the combined model proposed in this paper is superior to the single auto-regressive moving average model in different data sets, and the ARMA-SVR combined model can effectively predict the change of optical fiber frequency shift and provide a new idea for the prediction of rock pressure in the mining process.