bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental Validation of an <i>In Vitro</i> Method for Assessing Insulin-Dependent Glucose Uptake
Authors: Afanasyeva A. N. , Saparova V. B. , Makarenko I. E. , Drai R. V. , Selmenskikh T. A. 
Published: July 2022

Russian and international regulatory documents require that analytical procedures for establishing bioequivalence and comparability of quality attributes of biotechnological (biological) products, carrying out batch release of medicinal products, and conducting other equally important in vitro studies must be validated. These in vitro studies include molecule–receptor binding and product bioactivity assays. However, at present, there is no single approach to validation of in vitro bioanalytical methods not involving the determination of active ingredient concentrations in biological fluids. The aim of the work was to validate a procedure for assessing insulin-dependent glucose uptake and demonstrate the suitability of GOD-PAP GLUCOSE kits for glucose determination in culture media. Materials and methods: The study used RinFast® insulin aspart by Geropharm, Russia; a placebo for insulin aspart; the L6J1 rat myogenic cell line; and a GOD-PAP GLUCOSE kit for quantitative determination of glucose. The study was carried out on differentiated cells cultured for 7 days. To encourage L6J1 differentiation, the authors used DMEM with 4.5 g/L glucose and 2% horse serum. The statistical analysis of results was performed using Prism 9. Results: The study demonstrated the analytical procedure’s specificity, as the concentration of residual glucose in the culture medium observed with the placebo was 4 times higher than that with the maximum concentration of the medicinal product. The determination of precision showed the repeatability of 4–9% and the intralaboratory precision of 11–16%. The coefficient of variation for robustness amounted to 14% in 4 independent experiments comprising a total of 9 analytical runs. The authors compared insulin products (the insulin aspart and a genetically engineered human insulin), and the half-maximal inhibitory concentration (IC50) values differed by 1.5 times. For the GOD-PAP GLUCOSE kit, the linear regression coefficient of determination was 0.9983, the sensitivity amounted to 1 mmol/ L, and the accuracy ranged between 95% and 107%. Conclusions: the procedure using L6J1 rat myoblasts as a test system may be considered specific, highly precise, and robust in assessing insulin-dependent glucose uptake and suitable for detecting biological activity of insulin preparations in vitro.
Considerations for the Bioanalytical Part of Equivalence Studies of Biosimilar Nadroparin Calcium
Authors: Kosman V. M. , Karlina M. V. , Faustova N. M. , Ezhova E. A. , Kotelnikova I. G. , Makarov V. G. , Makarova M. N. 
Published: July 2022

According to current regulatory views, a comparative study of the pharmacodynamics (PD) of low molecular weight heparin (LMWH) products and confirmation of their equivalence require comparing three PD markers: the anti-Xa activity, the anti-IIa activity, and the tissue factor pathway inhibitor (TFPI) concentration. The aim of this study was to analyse the features specific to the bioanalytical part of an equivalence study of a nadroparin calcium biosimilar after single subcutaneous administration. Material and methods: the anti-Xa and anti-IIa activity values and TFPI content were determined in human plasma samples obtained after single subcutaneous administration of the test and the reference product in the same dose, using commercially available reagent kits and pre-validated assays. The authors calculated the main PD parameters (surrogate pharmacokinetic markers), namely the maximum activity or concentration (Amax or Cmax), time to maximum activity or concentration (Tmax), area under the activity–time (or concentration–time) curve (AUC ), and half-life period (T1/2), by means of model-independent statistical moment analysis and carried out further statistical testing of the parameters. Results: the anti-Xa activity and TFPI concentration results provided for the possibility of calculating and comparing the PD parameters (Amax or Cmax, AUC0-24, AUC0-∞, Tmax, T1/2) and estimating the confidence intervals that are necessary to confirm the bioequivalence of the studied products. The anti-IIa activity data had a characteristic pattern of slight fluctuations around one level, which prevented the calculation and comparison of PD parameters. Conclusion: the study identified specific features to consider when planning comparative PD studies of nadroparin calcium products. Firstly, it is feasible to divide samples into two test aliquots (one for anti-Xa and anti-IIa activity determination, the other for TFPI analysis) at the moment of collection in order to perform the analytical step correctly. Secondly, there is no need in full validation for the bioanalytical assays of the anti-Xa and anti-II activity and TFPI content in human plasma validated in the concentration ranges of 0.024–0.182 IU/mL, 0.0069–0.052 IU/mL and 1.56–100 ng/mL, respectively; a confirmation that the active ingredient does not interfere with the analytical procedure is adequate for the purpose. Finally, the data obtained may not allow for calculating PD parameters and comparing confidence intervals for all three markers. The listed considerations may be relevant for other LMWH products as well.
Characteristics, institutional behaviour, and post-release outcomes of federal Veteran and non-Veteran men offenders
Authors: MacDonald Shanna Farrell , Cram Sarah , Derkzen Dena , Pound Teresa , Mooz Mike 
Published: July 2022

LAY SUMMARY Incarcerated Veterans represent 2.5% of the federal offender population and are a unique subset of the general Canadian Veteran population. This study provides the first in-depth examination of Veteran offenders in federal custody. During the study period, 374 federal offenders self-reported as Veterans. Federal Veteran offenders were older and more likely to have committed a violent offence and to have mental health concerns. Although they were more likely to report mental health concerns, Veteran offenders have more stable institutional behaviour and greater post-release success than non-Veterans. Understanding the unique characteristics and correctional experiences of federal Veteran offenders aids in identifying needs related to intervention and support to promote successful community reintegration after release. Future qualitative research should enhance knowledge of the lived experiences of Veterans involved in the federal criminal justice system in Canada.
Mediating role of dyadic satisfaction on social support and health outcomes in a sample of military family caregivers
Authors: Wilcox Sherrie 
Published: July 2022

LAY SUMMARYMilitary family caregivers (MFCs) can experience many health-related challenges associated with caregiving responsibilities. Perceived support from a spouse or partner can influence dyadic satisfaction and health outcomes among those who care for a spouse or partner. This study examined the relations among social support, dyadic satisfaction, and mental (i.e., depressive and anxiety symptoms) and physical (i.e., somatic symptoms) health outcomes in a sample of MFCs. This sample consisted of 104 MFCs who were the spouse or partner of their care recipient. The findings indicate that more support predicted greater dyadic satisfaction and greater dyadic satisfaction predicted fewer anxiety symptoms. This study highlights the importance of considering relationship-focused contributors to mental health outcomes.
Władza Boga nad światem a ład moralny według Księgi Hioba
Authors: Tronina Antoni 
Published: July 2022

Nella cosmogonia vicino-orientale l’ordine politico si riflette in tutto il cosmo. In confronto eon tale concezzione del potere, Israele ha elaborato un altro modelio della creazione, armonizzato eon suo monoteismo rigoroso. Deutero-Isaia era propagatore precipuo di questa fede nel potere universale di Jahve. Il Libro di Giobbe, redatto nell’epoca dell’impero persiano, contiene un testimonio splendido del dibattimento sul probierna della ricompesa e della sofferenza. Immagini mitologici di combattimento delle divinità sono stati applicati ad illustrare il superamento di Jahvè sulle potenze del caos. La sapienza di Jahvè e il suo incrollabile governo guarantirono la stabilità del mondo creato e l’ordine morale. La giustizia (mishpat) divina costituisce fonadamento del potere politico e dell'ordine morale nel mondo.
Jezus wychowuje swoich uczniów w Mk 1,35-39
Authors: Kotecki Dariusz 
Published: July 2022

The article focuses on the issue of Jesus as the master educator of his disciples in Mark 1:35-39. The study provided is based on the principles of narrative and contextual analysis. The article consists of three parts: (1) Analysis of Mark 1:35-39; (2) Mk 1:35-39 in the context of 1:14-20; (3) Jesus master educator in 1:35-39 in the context of the Gospel of Mark. The pericope shows a certain tension between what Jesus must accomplish in terms of his mission given by the Father and what his disciples expect from him. The attitude of Simon and his companions (Andrew, Jacob and John) is expressed in Peter’s actions (“pursued him [Jesus]”) and in the apostles’ common words: ““Everyone is looking for you” (1:37). He shows that they have not yet reached the ideal personality as proposed by Jesus, which should be expressed in the attitude of repentance and faith (cf. 1:15). Their attempt to limit Jesus’ mission to Capernaum shows also that they are not yet ready to meet the goal of their vocation i.e. become fishers of men. In such context, through his command “Let us go somewhere else to the towns nearby, so that I may preach there also; for that is what I came for” (1:38), Jesus corrects their attitude and invites them to strengthen their bond with him in the perspective of a universal mission. In this way Jesus reveals himself as an educator, who encourages his disciples by his own example and his own words (encouragement, commands, reproaches, prohibitions and questions) to absolute clinging to him.
Janowe rozumienie pracy Boga i człowieka. Studium zastosowania terminów ἐργάζομαι oraz ἔργον w czwartej Ewangelii
Authors: Kubiś Adam 
Published: July 2022

The article deals with the concept of work as reflected in the Fourth Gospel, encompassing the two cognate lexemes ἐργάζομαι and ἔργον. The analysis is organized according to the subjects who are performing a given work, to wit: the Father, the Son, Jesus’ disciples (including Abraham) and, finally, the people hostile toward God, designated in the Gospel by the general term world. Interestingly enough, both of the studied lexemes appear in John’s Gospel only on the lips of Jesus. Additionally, as it turns out, the Johannine concept of work covers a whole range of meanings: the activity of the Father and the Son (who give life and perform judgment), the salvific mission of the Son, Jesus’ teaching and his obedience to the Father’swill, miraculous healings, human deeds in general (both good or bad), disciples’ fulfillment of God’s will, meaning primarily their faith in Jesus as the Messiah and the Son of God. In a nutshell, the very essence of the work of the Father and the Son (and continued by Jesus’ disciples) can be encapsulated in the concept of salvation.
Dotyk miłosierdzia (Mk 2,1-12)
Authors: Malina Artur 
Published: July 2022

Le guarigioni operate da Gesu diventano occasione per dimostrare il suo potere sul male che opprime gli uomini. Il senso di questi miracoli non si limita alla liberazione degli uomini da mali fisici. L'autorita di liberare gli uomini dai peccati viene inquadrata nel contesto dell'attivita taumaturgica a benificio degli infermi. La direzione della trasmissione di contagiosita dall'impuro al puro, riaffermata frequentemente nella Scrittura, e invertita di cento ottanta gradi: il lebbroso invece di contaminare Gesu, diventa purificato immediatamente dopo essere toccato da lui (1,41-42). L'accusa di bestemmiare, motivata dagli scribi con l'unicita di Dio (2,6-7), deve cedere al riconoscimento della sua autorita di rimettere i peccati, avvalorata dalla guarigione del paralitico (2,8-11). Alla contestazione del suo comportamento nei confronti dei peccatori egli rivela lo scopo di tutta la sua missione di salvezza nei loro confronti (2, 15-17).
Study of the needs and pharmaceutical provision of patients with diabetes during time of state of martial law
Authors: Власенко І. О. , Комаріда О. О. , Давтян Л. Л. 
Published: July 2022

Military action is a global health problem. Disruption of health care and inadequate pharmaceutical provision can lead to serious complications and even death of the patient, making the population particularly vulnerable. Emergency care always focuses on injuries, infectious diseases and other acute conditions, and chronic diseases, including diabetes, have historically been a low priority.
The aim of the work was to study the needs and problems of patients with diabetes, which arose during the martial law in the dynamics and to outline the main aspects of recommendations for improving the treatment of patients with diabetes in humanitarian crisis.
Materials and methods. The analysis of the needs and problems of patients with diabetes was studied by a questionnaire (February‒May 2022). People with diabetes and members of their families took part in the study. Relevant official documents and international recommendations about providing diabetic care to the population during crisis situations are analyzed. Content analysis, bibliosemantic, analytical, generalizing analyzes were used.
The studied needs and problems of patients with diabetes during martial law and their dynamics depending on time. Restrictions on medical care and rapid resumption of the pharmacy, which released, including insulin, have been established. The dynamics of actions on medical and pharmaceutical provision of patients with diabetes were analyzed, which showed that the health care system was not ready for martial law, but the Government took urgent appropriate actions on medical and pharmaceutical provision of patients with diabetes, which allowed to provide continuous treatment of patients with diabetes. The acute problem of not being able to supply the necessary drugs, including insulin, clean water, food to the occupied territories, creates a critical situation for the population in these regions, including those with diabetes, which can be fatal.
Based on the elaboration of international documents, the main aspects of recommendations for improving the medical and pharmaceutical provision of patients with diabetes in emergencies are outlined.
a KAJIAN KANDUNGAN HARA PADA BEBERAPA PENGGUNAAN LAHAN PERKEBUNAN DI KABUPATEN KUANTAN SINGINGI
Authors: OKALIA DENO 
Published: July 2022

Penggunaan berbagai lahan berpengaruh terhadap sifat kimia tanah terutama dengan keberadaan bahan organik tanah dan hara yang berbeda. Penelitian ini bertujuan untuk mengkaji kandungan hara pada berbagai penggunaan lahan perkebunan di kabupaten Kuantan singingi. Penelitian ini telah dilakukan dengan menggunakan metode survai. Sampel tanah diambil berdasarkan penggunaan lahan yaitu: a.Hutan primer (hutan lindung Sentajoraya), b.Perkebunan kelapa sawit selama 2 tahun, c.Perkebunan kelapa sawit selama 4 tahun, d.Perkebunan kelapa sawit selama 6 tahun, e.Perkebunan karet selama 2 tahun, f.Perkebunan karet selama 4 tahun, g.Perkebunan karet selama 6 tahun. Berdasarkan penelitian dapat disimpulkan bahwa ada pengaruh beberapa penggunaan lahan hutan, perkebunan kelapa sawit dan karet yang menunjukan terjadinya perubahan sifat kimia tanah diantaranya: kandungan Nilai C-organik pada tanah hutan 3,30% (kriteria tinggi), lahan kelapa sawit 1,53% - 2,47% (rendah sampai sedang), dan perkebunan karet 0,82% -2,78% (sangat rendah sampai sedang). Nilai Nitrogen (N) tanah pada lahan hutan 0,39%N (kriteria sedang), lahan kelapa sawit tanah menjadi 0,15- 0,23% (rendah sampai sedang), dan perkebunan karet menjadi 0,09% -0,24% (sangat rendah sampai sedang). Nilai analisis fosfor (P) pada tanah hutan 19,80 ppm (sedang), perkebunan kelapa sawit menjadi 14,55% P (umur 6 tahun) ppm - 16,47 (umur 2 tahun) ppm kategori rendah sampai sedang, perkebunan karet (P) tanah menjadi 12,67 (umur 2 tahun) ppm - 18,20 (umur 4 tahun) ppm kategori rendah sampai sedang. Nilai analisis K-dd tanah pada lahan hutan 0,65 me/100 gr kategori tinggi, perkebunan kelapa sawit 0,42 (umur 2 tahun) me/gr - 0,54 (umur 4 tahun) me/gr kategori sedang,dan perkebunan karet menjadi 0,39 (umur 2 tahun) me/gr sampai dengan 0,62 (umur 4 tahun) me/gr kategori sedang sampai tinggi.
SISTEM INFORMASI DESA SIALANG PANJANG KECAMATAN TEMBILAHAN HULU BERBASIS WEB
Authors: Disnasari Rita , Rasyid Ridha Muh. 
Published: July 2022

Until now, the Sialang Panjang Village Office does not yet have a special system to assist office staff in providing services to the community. However, there are several reasons why the Sialang Panjang Village Office wants a village system. These reasons include: they do not have a special program that is integrated into the database in terms of community population administration, besides the need for information for the local village community and the wider community is very large, many village communities do not know about village potential, village statistics, and the latest information. village. To overcome this, we need a software development process in the form of a Sialang Panjang Village Information System. The processes carried out are requirements analysis, system design, system implementation, system testing and system maintenance. The development method used is the waterfall method, while the analytical method used is the pieces method which can be a reference in building an information system for the village of Silang Panjang. UML (Unified Modeling Language) as a system modeling language, PHP (Hypertext Preprocessor) as a programming language and MySQL database. Then for system testing using black-box testing, white-box testing and questionnaires. With this village information system, it will be able to assist village office staff in providing services to the community and make it easier for the community to take care of population administration and obtain information.
Keywords: Web-Based Information System, Village Service, Sialang Panjang Village
Metagenomic Predictions: A Review 10 years on
Authors: Ross Elizabeth M , Hayes Ben J 
Published: July 2022

Metagenomic predictions use variation in the metagenome (microbiome profile) to predict the unknown phenotype of the associated host. Metagenomic predictions were first developed 10 years ago, where they were used to predict which cattle would produce high or low levels of enteric methane. Since then, the approach has been applied to several traits and species including residual feed intake in cattle, and carcass traits, body mass index and disease state in pigs. Additionally, the method has been extended to include predictions based on other multi-dimensional data such as the metabolome, as well to combine genomic and metagenomic information. While there is still substantial optimisation required, the use of metagenomic predictions is expanding as DNA sequencing costs continue to fall and shows great promise particularly for traits heavily influenced by the microbiome such as feed efficiency and methane emissions.
Intestinal Microbiota in Colorectal Adenoma-Carcinoma Sequence
Authors: Hua Hanju , Sun Yanhong , He Xinjue , Chen Yi , Teng Lisong , Lu Chao 
Published: July 2022

BackgroundMost colorectal cancer (CRC) cases are sporadic and develop along the adenoma-carcinoma sequence. Intestinal microbial dysbiosis is involved in the development of colorectal cancer. However, there are still no absolute markers predicting the progression from adenoma to carcinoma. This study aimed to investigate the characteristics of intestinal microbiota in patients with colorectal adenoma and carcinoma and its correlations with clinical characteristics.MethodsFecal samples were collected from 154 patients with CRC, 20 patients with colorectal adenoma (AD) and 199 healthy controls. To analyze the differences in the intestinal microbiota, 16S rRNA gene sequencing was conducted.ResultsAt the genus level, there were four significantly different genera among the three groups, namely Acidaminococcus, Alloprevotella, Mycoplasma, and Sphingobacterium, while Acidaminococcus significantly decreased with the order of Control-AD-CRC (P &lt; 0.05). In addition, Parvimonas, Peptostreptococcus, Prevotella, Butyricimonas, Alistipes, and Odoribacter were the key genera in the network of colorectal adenoma/carcinoma-associated bacteria. The top 10 most important species, including Butyricimonas synergistica, Agrobacterium larrymoorei, Bacteroides plebeius, Lachnospiraceae bacterium feline oral taxon 001, Clostridium scindens, Prevotella heparinolytica, bacterium LD2013, Streptococcus mutans, Lachnospiraceae bacterium 19gly4, and Eubacterium hallii, showed the best performance in distinguishing AD from CRC (AUC = 85.54%, 95% CI: 78.83–92.25%). The clinicopathologic features, including age, gender, tumor location, differentiation degree, and TNM stage, were identified to be closely linked to the intestinal microbiome in CRC.ConclusionSeveral intestinal bacteria changed along the adenoma-carcinoma sequence and might be the potential markers for the diagnosis and treatment of colorectal adenoma/carcinoma. Intestinal microbiota characteristics in CRC should account for the host factors.