bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D microfluidics-assisted modeling of glucose transport in placental malaria
Authors: Mosavati Babak , Oleinikov Andrew , Du E. 
Published: September 2022

AbstractThe human placenta is a critical organ, mediating the exchange of nutrients, oxygen, and waste products between fetus and mother. Placental malaria (PM) resulted fromPlasmodium falciparuminfections causes up to 200 thousand newborn deaths annually, mainly due to low birth weight, as well as 10 thousand mother deaths. In this work, a placenta-on-a-chip model is developed to mimic the nutrient exchange between the fetus and mother under the influence of PM. In this model, trophoblasts cells (facing infected or uninfected blood simulating maternal blood and termed “trophoblast side”) and human umbilical vein endothelial cells (facing uninfected blood simulating fetal blood and termed “endothelial” side) are cultured on the opposite sides of an extracellular matrix gel in a compartmental microfluidic system, forming a physiological barrier between the co-flow tubular structure to mimic a simplified maternal–fetal interface in placental villi. The influences of infected erythrocytes (IEs) sequestration through cytoadhesion to chondroitin sulfate A (CSA) expressed on the surface of trophoblast cells, a critical feature of PM, on glucose transfer efficiency across the placental barrier was studied. To create glucose gradients across the barrier, uninfected erythrocyte or IE suspension with a higher glucose concentration was introduced into the “trophoblast side” and a culture medium with lower glucose concentration was introduced into the “endothelial side”. The glucose levels in the endothelial channel in response to CSA-adherent erythrocytes infected with CS2 line of parasites in trophoblast channel under flow conditions was monitored. Uninfected erythrocytes served as a negative control. The results demonstrated that CSA-binding IEs added resistance to the simulated placental barrier for glucose perfusion and decreased the glucose transfer across this barrier. The results of this study can be used for better understanding of PM pathology and development of models useful in studying potential treatment of PM.
Posts on central websites need less originality to be noticed
Authors: Coscia Michele , Vandeweerdt Clara 
Published: September 2022

AbstractInformation has major consequences for democracy and society. It is important to understand what factors favor its diffusion. The impact of the content of a message on its likelihood of going viral is poorly understood. Some studies say originality is important for a message not to be overlooked. Others give more relevance to paratextual elements—network centrality, timing, human cognitive limits. Here we propose that originality and centrality interact in a nontrivial way, which might explain why originality by itself is not a good predictor of success. We collected data from Reddit on users sharing hyperlinks. We estimated the originality of each post title and the centrality of the website hosting the shared link. We show that the interaction effect exists: if users share content from a central website, originality no longer increases the odds of receiving at least one upvote. The same is not true for the odds of becoming one of the top 10% scoring posts. We show that originality is concentrated in the domain network: domains in the core of the network produce more original content. Our results imply that research on online information virality needs to take into account the nontrivial interaction between originality and prominence.
Time to CT scan for patients with acute severe neurological symptoms: a quality assurance study
Authors: Pape Pernille , Jensen Alice Herrlin , Bergdal Ove , Munch Tina Nørgaard , Rudolph Søren Steemann , Rasmussen Lars Simon 
Published: September 2022

AbstractEmergent brain computed tomography (CT) scan allows for identification of patients presenting with acute severe neurological symptoms in whom medical and surgical interventions may be lifesaving. The aim of this study was to evaluate if time to CT from arrival at the emergency department exceeded 30 min in patients admitted with acute severe neurological symptoms. This was a retrospective register-based quality assurance study. We identified patients admitted to the emergency department with acute severe neurological symptoms between April 1st, 2016 and September 30th, 2020. Data were retrieved from the registry of acute medical team activations. We considered that time to CT from arrival at the emergency department should not exceed 30 min in more than 10% of patients. A total of 559 patients were included. Median time from arrival at the emergency department until CT scan was 24 min (IQR 16–35) in children (< 18 years), 10 min (IQR 7–17) for adults (18–59 years), and 11 min (IQR 7–16) for elders (> 60 years). This time interval exceeded 30 min for 8.2% (95% CI 6.1–10.9) of all included patients, 35.3% of children, 5.9% of adults, and 8.6% of elders. No children died within 30 days. The 30-day mortality was 21.3% (95% CI 16.4–27) in adults, and 43.9% (95% CI 38.2–49.8) in elders. Time from arrival at our emergency department until brain CT scan exceeded 30 min in 8.2% of all included patients but exceeded the defined quality aim in children and could be improved.
Effects of virtual body-representation on motor skill learning
Authors: Shin Yongmin , Lim Jaeseo , Kim Yonggwan , Seo Deog-Gyu , Ihm Jungjoon 
Published: September 2022

AbstractMotor learning is often hindered or facilitated by visual information from one’s body and its movement. However, it is unclear whether visual representation of the body itself facilitates motor learning. Thus, we tested the effects of virtual body-representation on motor learning through a virtual reality rotary pursuit task. In the task, visual feedback on participants’ movements was identical, but virtual body-representation differed by dividing the experimental conditions into three conditions: non-avatar, non-hand avatar, and hand-shaped avatar. We measured the differences in the rate of motor learning, body-ownership, and sense of agency in the three conditions. Although there were no differences in body-ownership and sense of agency between the conditions, the hand-shaped avatar condition was significantly superior to the other conditions in the rate of learning. These findings suggest that visually recognizing one’s body shape facilitates motor learning.
Biochemical characterization of a disease-causing human osteoprotegerin variant
Authors: Luo Yin , Li Miaomiao , Xu Ding 
Published: September 2022

AbstractRecently, a human mutation of OPG was identified to be associated with familial forms of osteoarthritis. This missense mutation (c.1205A =  > T; p.Stop402Leu) occurs on the stop codon of OPG, which results in a 19-residue appendage to the C-terminus (OPG+19). The biochemical consequence of this unusual sequence alteration remains unknown. Here we expressed OPG+19 in 293 cells and the mutant OPG was purified to homogeneity by heparin affinity chromatography and size exclusion chromatography. We found that in sharp contrast to wildtype OPG, which mainly exists in dimeric form, OPG+19 had a strong tendency to form higher-order oligomers. To our surprise, the hyper-oligomerization of OPG+19 had no impact on how it binds cell surface heparan sulfate, how it inhibits RANKL-induced osteoclastogenesis and TRAIL-induced chondrocytes apoptosis. Our data suggest that in biological contexts where OPG is known to play a role, OPG+19 functions equivalently as wildtype OPG. The disease-causing mechanism of OPG+19 likely involves an unknown function of OPG in cartilage homeostasis and mineralization. By demonstrating the biochemical nature of this disease-causing OPG mutant, our study will likely help elucidating the biological roles of OPG in cartilage biology.
TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum
Authors: Shimomura Kenji , Ino Soshi , Tamura Kazuya , Terajima Takehito , Tomizawa Motohiro 
Published: September 2022

AbstractThe sensory perception of irritant chemicals results in escape and repellency behavioral patterns in insects. Transient receptor potential channels are cation channels that function as sensor proteins for several types of signals, such as light, sound, temperature, taste, as well as chemical and physical stimuli; among these, the TRPA channel is widely conserved and activated by irritant chemicals. Certain plant-derived essential oils (EOs), produced by secondary metabolism, are mixtures of volatile compounds, which are used as repellents because they contain environmentally sustainable ingredients. Citronellal, which is present in citronella EO from Cymbopogon species, is a potentially viable insect repellent; however, the repellency capability against coleopteran beetles remains limited. We investigated the citronellal-derived repellency behavior for the red flour beetle Tribolium castaneum, in which TcTRPA1 and odorant receptor co-receptor (Orco) expressions were mediated by RNA interference. Area-preference tests showed dose-dependent repellency behavior for citronellal; additionally, both TcTRPA1 and TcOrco double-strand RNA (dsRNA) micro-injection showed clear TcTRPA1 and TcOrco transcript reductions, and only TcTRPA1 dsRNA treatment significantly impaired repellency behavior. The relative expression level of the TcTRPA1 transcripts, evaluated by quantitative reverse-transcription PCR (qRT-PCR), revealed dominant expression in the antennae, indicating the antennae-expressed TcTRPA1-mediated repellency behavior.
Real-time energy consumption and air pollution emission during the transpacific crossing of a container ship
Authors: Yeh Chin-Ko , Lin Chitsan , Shen Hsueh-Chen , Cheruiyot Nicholas Kiprotich , Nguyen Duy-Hieu , Chang Chi-Chung 
Published: September 2022

AbstractThis study presents the real-time energy consumption of a container ship’s generator engine on two round-trips from the West Coast of the US to the East Asian ports and analyzes the ship’s PM10, PM2.5, NOx, SOx, CO, and HC emissions, shore power usage, and factors affecting energy consumption. The average total energy consumption and air emissions for the two round trips were 1.72 GWh and 42.1 tons, respectively. The transpacific crossing segment had the highest average energy consumption (2848 ± 361 kWh) and pollutant emission rate (78.9 ± 10.0 kg h−1). On the other hand, the West Coast of the US had the least energy consumption due to shore power adoption. Furthermore, switching from heavy fuel oil (HFO) to ultra-low-sulfur fuel oil (ULSFO) greatly reduced the emissions of PM and SOxby > 96% and NOxby 17.0%. However, CO and HC increased by 16.9% and 36.1%, respectively, implying incomplete combustion. In addition, the energy consumption was influenced by the number of reefers and wind. Therefore, this study recommends further research on energy-efficient reefers, generator engine optimization, and shore power adoption to reduce emissions from container ships.
Morphometric assessment of the left inferior phrenic vein in patients with portal hypertension
Authors: Fujii Yoshimi , Koizumi Jun , Sekiguchi Yuka , Ono Shun , Sekiguchi Tatsuya , Hara Takuya , Hashimoto Jun 
Published: September 2022

AbstractThe left inferior phrenic vein (LIPV) is a major drainage vessel of gastric varices and serves as an important conduit in endovascular treatment for gastric varices. The narrowing of LIPV has been empirically demonstrated and sometimes hinders catheter insertion for the treatment of gastric varices. We herein investigated the morphology of narrowed LIPV in patients with portal hypertension. Venograms of LIPV on 25 patients with gastric varices (15 males; 10 females; age range, 45–79 years with a mean of 67 years) were retrospectively reviewed, the following four parameters were measured: the diameter of LIPV, the diameter of narrowed LIPV, the narrowing rate, and the distance to narrowed LIPV from the left renal vein. On all 25 venograms, a narrowing was detected just above the common trunk with the left adrenal vein. The diameter of LIPV was 9.0 ± 4.2 mm, the diameter of narrowed LIPV was 5.1 ± 2.3 mm, the narrowing rate was 40.6 ± 16.0%, and the distance to narrowed LIPV from the left renal vein was 20.0 ± 7.4 mm. This anatomical information about the narrowing of LIPV may contribute to the safe and efficacious treatment of gastric varices.
Toll-like receptor 4-mediated endoplasmic reticulum stress induces intestinal paneth cell damage in mice following CLP-induced sepsis
Authors: Wang Yijie , Zhang Dapeng , Li Congxin , Wu Xue , He Chen , Zhu Xiaolin , Zhao Haiyan , Mu Lingjie 
Published: September 2022

AbstractA marked elevation of TLR4 was observed in various organs of septic mice. The mechanism of TLR4 in intestinal epithelial cell damage in sepsis remains unclear. CLP mice models were used to assess the role of TLR4 in intestinal Paneth cell damage by histological, polymerase chain reaction, western-blot analyses. The ileal expression of TLR4 was increased by more than five-fold after CLP. CLP significantly increased 7-day mortality and was associated with a higher murine sepsis score (MSS), closely related with increased TLR4 expression. Histological staining revealed that a reduced number of Paneth cells, accompanied by reduced lysozyme and defensin alpha 5(DEF-5) expression as detected by PCR. Of note, the expression levels of ATF6, XBP1 and CHOP increased in the ileal of the sepsis group. Meanwhile, the uncleaved p90 ATF6 was markedly reduced and cleaved p50 ATF6 was increased in the sepsis group. Intriguingly, The TAK-242 had improved intestinal mucosal injury, reduced the expression of ATF6, XBP1 and CHOP and relieved the cleavage of ATF6. We found that increased the expression level of TLR4 in the ileal of CLP mice promoted the depletion of Paneth cell and reduced LYZ and DEF-5 expression. Furthermore, our findings suggested that TLR4-mediated the hyperactivation of ER stress, via activating the ATF6/CHOP pathway, might be one of the mechanisms associated with Paneth cells loss and dysfunction during intestinal barrier impairment of sepsis.
The effect of gelatin as pore expander in green synthesis mesoporous silica for methylene blue adsorption
Authors: Ulfa Maria , Prasetyoko Didik , Trisunaryanti Wega , Bahruji Hasliza , Fadila Zahra Ayu , Sholeha Novia Amalia 
Published: September 2022

AbstractMesoporous silica NSG had been synthesized while employing gelatin as a natural template to successfully increase the particle size and expand the pore diameter of NSG. All silica samples exhibited a similar XRD pattern with a broad peak centred at 2θ = 22.9°, as the characteristic of amorphous silica. FTIR results showed that the reduction of Si–O–Si symmetric stretching vibrations at 1075 cm−1 was due to the use of a high percentage of gelatin. Moreover, TEM analysis displayed the mesoporous channels in the form of a honeycomb structure with a diameter of ± 6 nm. Gelatin enhanced the surface area of silica from 467 to 510 m2/g, the pore volume from 0.64 to 0.72 cc/g and expanded the pore diameter from 3.5 nm to 6.0 nm. The expansion of the ordered mesopores with the increase of P123: gelatin ratios was elucidated by the pore size distribution. The adsorption capacity of methylene blue (MB) was improved on mesoporous silica with an expanded pore dimension to give 168 mg/g adsorption capacity within 70 min.
Characteristic distribution and molecular properties of normal cellular prion protein in human endocrine and exocrine tissues
Authors: Koyama Sachiko , Noguchi Hideko , Yagita Kaoru , Hamasaki Hideomi , Shijo Masahiro , Yoshimura Motoi , Inoshita Kohei , Sasagasako Naokazu , Honda Hiroyuki 
Published: September 2022

AbstractPrion disease is an infectious and fatal neurodegenerative disease. Human prion disease autopsy studies have revealed abnormal prion protein (PrPSc) deposits in the central nervous system and systemic organs. In deer, chronic wasting disease has also become a global problem, with PrPSc in saliva and feces. Therefore, understanding normal cellular prion proteins (PrPc) characteristics in human systemic organs is important since they could be a PrPSc source. This study used western blotting and immunohistochemistry to investigate endocrine and exocrine tissues, such as the human pituitary, adrenal, submandibular glands and the pancreas. All tissues had 30–40 kDa PrP signals, which is a slightly higher molecular weight than normal brain tissue. Most cytoplasmic PrP-positive adenohypophyseal cells were immunopositive for nuclear pituitary-specific positive transcription factor 1. The adrenal medulla and islet cells of the pancreas were PrP-positive and colocalized with chromogranin A. The duct epithelium in the submandibular gland and pancreas were immunopositive for PrP. This study reports the characteristic molecular properties and detailed tissue localization of PrPc in endocrine and exocrine tissues, which is important for infection control and diagnosis.
Synthesis of vinyl-based silica nanoparticles by sol–gel method and their influences on network microstructure and dynamic mechanical properties of nitrile rubber nanocomposites
Authors: Ghamarpoor Reza , Jamshidi Masoud 
Published: September 2022

AbstractNon-agglomeration and dispersion of silica nanoparticles in polymers and their interfacial interactions to polymer matrix are the most important factors that influence nanocomposites performance. In this work, vinyltriethoxysilane (VTES) as a low VOC emission coupling agent was used for surface modification of silica nanoparticles to prepare better dispersion in nitrile rubber (NBR) and improve its interfacial interactions to silica nanoparticles. The results of X-ray photoelectron spectroscopy, thermogravimetric analysis and Fourier transform infra-red spectroscopy demonstrated successful attachment of VTES molecules on the surface of silica nanoparticles. Dispersion of the modified silica nanoparticles in NBR matrix was studied using field emission scanning electron microscopy and rubber process analysis. Results demonstrated that VTES significantly improved dispersion of nanoparticles in rubbery matrix. The bound rubber content showed that VTES effectively built a bridge between the silica nanoparticles and the rubber matrix that led to promising mechanical performances and strong interfacial interactions. Effect of nanoparticle content on the mechanical performances (static/dynamic) of the NBR was evaluated. It was found that higher modulus and reinforcement indices was obtained at 3 and 5 wt% of nanoparticles. Moreover, these composites had extremely low rolling resistance, the best wet skid resistance and the lowest Heat-Build up.
The prevention of home-cage grid climbing affects muscle strength in mice
Authors: Ueno Hiroshi , Takahashi Yu , Murakami Shinji , Wani Kenta , Miyazaki Tetsuji , Matsumoto Yosuke , Okamoto Motoi , Ishihara Takeshi 
Published: September 2022

AbstractExperimenters and treatment methods are the major contributors to data variability in behavioral neuroscience. However, home cage characteristics are likely associated with data variability. Mice housed in breeding cages spontaneously exhibit behavioral patterns such as biting into the wire grid and climbing on the grid lid. We aimed to clarify the effect of covering the stainless steel wire grid lid in commonly used home cage with Plexiglas to prevent climbing on muscle strength in mice. Furthermore, we investigated the effects of climbing prevention on activity and anxiety-like behavior, and the impact of climbing prevention during the postnatal development period and adulthood on muscle strength. Muscle strength, anxiety-like behavior, and locomotor activity were assessed by a battery of tests (wire hang, suspension, grip strength, rotarod, elevated-plus maze, and open field tests). Mice prevented from climbing the wire grid during postnatal development displayed lower muscle strength than those able to climb. Moreover, mice prevented from climbing for 3 weeks following maturity had weakened muscles. The muscle strength was decreased with 3 weeks of climbing prevention in even 1-year-old mice. In summary, the stainless steel wire grid in the home cage contributed to the development and maintenance of muscle strength in mice.
The effect of maternal anaemia on low birth weight among newborns in Northwest Ethiopia
Authors: Engidaw Melaku Tadege , Eyayu Tahir , Tiruneh Tegenaw 
Published: September 2022

AbstractLow birth weight is an indicator of maternal-related multifactorial problems such as malnutrition, illness, and work overload. As a result, low birth weight is associated with maternal anaemia, and both of them were significant public health issues in developing nations. Low birth weight and anaemia are caused by insufficient nutrient intake, which is especially severe during pregnancy. So, this study aimed to assess the effect of maternal anaemia during the late trimester on low birth weight among newborns in Northwest Ethiopia. A systematic random sampling technique was used to select 211 participants for the primary data collection. Face-to-face interviews were used to collect data, while blood samples were collected using standard operating procedures. For further analysis, the data file was imported into Stata version 16 (MP) software. The binary logistic regression model was used to investigate significant factors related to low birth weight. Finally, the statistical significance of the variables was determined using a p value of ≤ 0.05. The prevalence of anaemia among pregnant women in the late trimester and newborns was 34 (16.11%, 95% CI: 11.42, 21.78) and 64 (30.33%, 95% CI: 24.20, 37.01), respectively. The mean ± standard deviation of the newborn babies' weight was 3.19 ± 0.49 kg. The proportion of low birth weight among newborns was 26 (12.32%, 95% CI: 8.20, 17.53%). The independent effect of anaemia on low birth weight was 4.19 times while all other factors were constant (COR = 4.19, 95% CI: 1.70, 10.30). Maternal educational status [unable to read and write (AOR = 10.94, 95% CI: 1.74, 68.58) and attained secondary education (AOR = 8.06, 95% CI: 1.53, 42.36)], and maternal anaemia (AOR = 3.51, 95% CI: 1.29, 9.55) were associated with low birth weight after adjusting with all other variables. In this study, the proportion of low birth weight was high. Here, maternal anaemia alone had a significant independent role in the development of low birth weight. Maternal education status and anaemic conditions were associated with low birth weight among newborns. Early detection and treatment of maternal anaemia during pregnancy is crucial with the usual nutritional-related care.