bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioinformatics Analysis of Kinesin Family Member 2C in Human Tumors: Novel Prognostic Biomarker and Tumor Microenvironment Regulator
Authors: Zhang Bixi , Li Yanchun , Pang Xiaoyang , Wu Hao 
Published: July 2022

Abstract
Kinesin family member 2C (KIF2C) is the best-characterized member of the kinesin-13 family and is involved in accurately fine-tuned dynamics of mitotic spindles. As KIF2C is involved in both spindle formation and regulation of DNA double-strand breaks, precise regulation of KIF2C is essential to prevent malignant transformation associated with gains and losses of DNA content. In the present study, we initially reviewed The Cancer Genome Atlas database and observed that KIF2C is abundantly expressed in most tumor types. We then analyzed the gene alteration profile, protein expression, prognosis, and immune reactivities of KIF2C in more than 10,000 samples from several well-established databases. In addition, we conducted a gene enrichment set analysis to investigate the potential mechanisms underlying the role of KIF2C in tumorigenesis. Pan-cancer analysis of KIF2C demonstrated significant statistical correlations between KIF2C expression and clinical prognosis, oncogenic signature gene sets, myeloid-derived suppressor cell infiltration, ImmunoScore, immune checkpoints, microsatellite instability, and tumor mutational burden across multiple tumors. Single-cell data showed that KIF2C is abundantly expressed in malignant cells. The findings of this study provide important insight for understanding the role and mechanisms of KIF2C in tumorigenesis and immunotherapy in a variety of cancers.
Enhancing COVID Rehabilitation with Technology (ECORT): Protocol for an open-label, single site randomized controlled trial evaluating the effectiveness of electronic case management for individuals with persistent COVID-19 symptoms.
Authors: Hatcher Simon , Werier Joel , Edgar Nicole E , Booth James , Cameron William J , Corrales-Medina Vicente , Corsi Daniel , Cowan Juthaporn , Giguère Pierre , Kaluzienski Mark , Marshall Shawn , Mestre Tiago , Mulligan Bryce , Orpana Heather , Pontefract Amanda , Stafford Darlene , Thavorn Kednapa , Trudel Guy 
Published: July 2022

Abstract
Background: As of May 2022, Ontario has seen more than 1.3 million cases of COVID-19. While the majority of individuals will recover from infection within four weeks, a significant subset experience persistent and often debilitating symptoms, known as “post-COVID syndrome” or “Long COVID”. Those with Long COVID experience a wide array of symptoms, with variable severity, including fatigue, cognitive impairment, and shortness of breath. Further, the prevalence and duration of Long COVID is unclear, nor is there evidence on the best course of rehabilitation for individuals to return to their desired level of function. Previous work with chronic conditions has suggested that the addition of electronic case management (ECM) may help to improve outcomes. These platforms provide enhanced connection with care providers, detailed symptom tracking and goal setting, and access to relevant resources. In this study, our primary aim is to determine if the addition of ECM with health coaching improves Long COVID outcomes at three months compared to health coaching alone.Methods: The trial is an open-label, single site, randomized controlled trial of ECM with health coaching (ECM+) compared to health coaching alone (HC). Both groups will continue to receive usual care. Participants will be randomized equally to receive health coaching (+/- ECM) for a period of 8 weeks and a 12-week follow-up. Our primary outcome is the WHO Disability Assessment Scale (WHODAS), 36-item self-report total score. Participants will also complete measures of cognition, fatigue, breathlessness, and mental health. Participants and care providers will be asked to complete a brief qualitative interview at the end of the study to evaluate acceptability and implementation of the intervention.Discussion: There is currently little evidence about the optimal treatment of Long COVID patients or the use of digital health platforms in this population. The results of this trial could result in rapid, scalable, and personalized care for people with Long COVID which will decrease morbidity after an acute infection. Results from this study will also inform decision making in Long COVID and treatment guidelines at provincial and national levels.Trial registration: ClinicalTrials.gov, NCT05019963, Registered on 25 August 2021, https://clinicaltrials.gov/ct2/show/NCT05019963
An Integrated Decision-Making Approach Based on Pythagorean Fuzzy Sets to analyze the wearable app issues
Authors: Pandey Mamta , Litoriya Ratnesh , Pandey Prateek 
Published: July 2022

Abstract
In recent years, there has been an explosion in the popularity of wearable applications (wearable apps). However, to this day, relatively few research have been conducted to investigate the issues that are presented by wearable apps. Prior research has shown that user evaluations may provide a wealth of information that can be utilized to better identify quality concerns and assist app developers create higher quality wearable apps. In light of this, the purpose of this article is to mine user evaluations in order to get an understanding of the consumer concerns about wearable apps. In this paper, fifteen issues related to wearable apps are analyzed and discussed. In this paper, fifteen wearable apps issues have been identified. Afterwards we used the DEMATEL approach (Decision Making Trial and Evaluation Laboratory) to examine and classify the issues with wearable apps. Initially, numerous experts evaluate the direct relation among the issues in wearable apps. Pythagorean fuzzy number (PFN) are used to represent the evaluation of outcomes and linguistic terms are converted into PFN. After that issues are classified in group of cause and effect based on DEMATEL method. And results are compared with other variants of DEMATEL including Pythagorean fuzzy DEMATEL and Rough z number DEMATEL. Comparative analysis indicates that Pythagorean Fuzzy DEMATEL is best approach to analyze the relationship among issues in wearable apps. The conclusion of this study undoubtedly aids the apps/software business in successfully identifying the challenges on which experts and project managers should concentrate.
Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source
Authors: Lu Qiyuan , Shan Xiaoyu , Zeng Weizhu , Zhou Jingwen 
Published: July 2022

Abstract
Pyruvic acid is an important organic acid and a key industrial raw material. It is widely used in the chemical, agricultural, and food fields. Candida glabrata is the preferred strain for pyruvic acid production. The waste yeast cell for pyruvic acid fermentation with C. glabrata are rich in protein, amino acid, nucleic acid, and vitamins, as potential and cost-effective nitrogen source raw material. In this study, the potential of C. glabrata to produce pyruvic acid using spent yeast cell dry powder was evaluated. When 30 g/L of spray-dried spent yeast cell powder was used as the seed nitrogen source, a high titer of pyruvic acid was obtained. The pyruvic acid production reached 63.4 g/L with a yield of 0.59 g/g in a 5-L bioreactor. After scale-up to a 50-L bioreactor using the fermented spent yeast cell dry powder as a seed nitrogen source, 65.1 g/L of pyruvic acid was harvested, with a yield of 0.61 g/g. This study proposes a promising approach for increasing the pyruvic acid titer and reducing the costs.
First Report of Evaluation of Variant rs11190870 near LBX1 with Adolescent Idiopathic Scoliosis Susceptibility in a South-Asian Indian Population
Authors: Sharma Swarkar , Singh Hemender , Kowra Shipra , Gupta Manish , Bhau Poorvi , Chalotra Tania , Manotra Ruchi , Gupta Nital , Gupta Geetanjali , Pandita Ajay , Butt Mohammad , Sharma Rajesh , Pandita Sarla , Singh Vinod , Garg Bhavuk , Rai Ekta 
Published: July 2022

Abstract
Variant rs11190870 is located 7.5 kb downstream of LBX1 gene and several studies have implicated its association with Adolescent Idiopathic Scoliosis (AIS) in different populations. However, any genetic study is altogether lacking in South-Asian Indian (SAI) populations. We have recently reported lower incidence of AIS in SAI populations. Based on it, a case-control association study was designed to carry out replication of variant rs11190870 in a SAI population. In the study, 95 AIS cases and 282 non-AIS controls from Northwest India were screened and the variant was found to be following Hardy Weinberg equilibrium but did not show significant association (p = 0.66) with AIS. Interestingly, differences in frequency distribution as well as Linkage Disequilibrium (LD) of the variant were observed in SAI populations than other populations in 1000 Genomes phase 3 data. The findings hint at genetic heterogeneity to AIS susceptibility in SAI populations, warranting further studies.
Intestinal microbiomics and metabolomics insights into the hepatoprotective effects of Lactobacillus paracasei CCFM1222 against the acute liver injury in mice
Authors: Guo Weiling , Cui Shumao , Tang Xin , Zhang Qiuxiang , Zhao Jianxin , Mao Bingyong , Zhang Hao 
Published: July 2022

Abstract
In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality. This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice, and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics. The results displayed that L. paracasei CCFM1222 pretreatment significantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice. The cecal short-chain fatty acid (SCFAs) levels were increased in LPS-treated mice with L. paracasei CCFM1222 pretreatment. In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bifidobacterium, and lower abundance of Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids. The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment significantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarkers-related butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism. Moreover, L. paracasei CCFM1222 pretreatment remarkably regulated the expression of genes-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1). In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has beneficial effects on preventing the occurrence and development of ALI by inhibiting the inflammatory responses, altering intestinal microbiota composition and their metabolites, and mediating the Tlr4/Nf-kβ pathway.
Sector Area Index: a novel marker for blepharoptosis screening and grading
Authors: Mahmoudi Tahereh , Riazi-Esfahani Hamid , Montazeriani Zahra , Yaseri Mehdi , Namdar Zahra Mehdipour , Rafizadeh Seyed Mohsen , Pour Elias Khalili 
Published: July 2022

Abstract
The purpose of current study was to introduce a new marker for discriminating different grades of ptosis called Sector Area Index (SAI) and a semi-automated technique to calculate it. In this cross-sectional comparative case series, after choosing two points as the medial and lateral canthus and manually selecting the palpebral fissure region, a circle enclosing the intercanthal distance was automatically drawn. Finally, 15-degree-apart sectors are applied to the enclosed circle. SAI was measured automatically by dividing the area of each 15-degree sector marked with the upper eyelid contour by the total area of the sector marked with the edge of the surrounding circle. SAI values and inter-eye SAI differences were compared between patients with different grades of ptosis as well as normal patients. One hundred six eyes were recruited (30, 25, 27, and 24 eyes in the control, mild, moderate, and severe ptosis groups, respectively). Mean values of SAI in all sectors showed a decreasing trend from normal individuals toward patients with severe ptosis. The mean difference values of SAI between study eyes and fellow eyes in all four groups of patients showed a statistically significant difference (p < 0.05). In a pairwise comparison between groups, mean values of SAI in all nasal sectors from 15°–60° showed a statistically significant difference between all groups (p < 0.05). In conclusion, SAI measurements in 15°–60° sectors can demonstrate differentiating performance for detecting and discriminating varying grades of ptosis.
Diagenesis analysis, Microfacies of Lower Dalan Formation, in one of gas field at South of Iran (Persian Gulf)
Authors: Vakili Nazanin , Paknejadiyan Ali , Yazdjerdi Kouros , mousavi seyed saeed mousapoor 
Published: July 2022

Abstract
This research studies a subterraneous section of the Dalan Formation in well X in one of the Persian Gulf gas field. The Dalan Formation, Permian in age, in folded Zagros region and the Persian Gulf contains giant gas resources. The lower boundary of the Dalan Formation is continuous and gradual, along with the siliciclastics of Faraghun Formation. The Upper Dalan is overlain by the Kangan Formation. The boundary of these Formations is the Permo-Triassic erosional surface. Diagenesis processes that affected the Dalan Formation include Cementation, Bioturbation, Micritization, Geopetal fabric, Dissolution, Compaction, Neomorphism, Pyritization, Ironzation and Dolomitization. Dolomitization, dissolution and cementation are the predominant diagenetic processes in this Formation. Marine, Meteoric and Burial diagenetic environments affected the Dalan Formation. According to sedimentary structure, texture and biota content 16 microfacies are determined for the Dalan Formation. These microfacies are presented in 3 facies belts, including Tidal flats, Lagoon and Carbonate shoal. The sedimentary environment of the Dalan Formation is introduced as a homoclinal carbonate ramp deposition. Dissolution along with microfractures had a positive effect on the improvement of reservoir quality.
Vinpocetine attenuates diabetic cardiomyopathy by downregulating transforming growth factor-1 and phosphodiesterase-1 expression in rats
Authors: Vishwakarma Vishal Kumar , Shah Sadia , Kaur Tajpreet , Singh Amrit Pal , Araya Sudheer Kumar , Kumar Niraj , Kanwar Raj , Yadav Sushma , Arora Taruna , Yadav Harlokesh Narayan 
Published: July 2022

Abstract
Diabetic cardiomyopathy is one of the serious secondary complications of diabetes mellitus in humans. Existing therapy affords symptomatic relief without halting disease progression. Vinpocetine is an alkaloid having pleiotropic pharmacological effects. Present study is designed to investigate the effect of vinpocetine in diabetic cardiomyopathy in rats. Rats were fed with high fat diet (HFD) for nine weeks along with streptozotocin (35 mg/kg, intraperitoneal) after second week to induce diabetic cardiomyopathy. At the end of protocol, echocardiography of rats was done. Morphological parameters including body weight, heart weight was noted. Rat heart were evaluated for functional status (left ventricular developed pressure, dp/dtmax, dp/dtmin) using Biopac system. Cardiac biochemical (creatine kinase-MB, lactate dehydrogenase, aspartate aminotransferase) oxidative stress parameters (malondialdehyde, superoxide dismutase, reduced glutathione, catalase) and inflammatory cytokine (tumor necrosis factor-α, interleukin-6) level, were analysed in addition to haematoxylin eosin and picrosirius red staining to study histological changes, cardiomyocyte diameter and fibrosis, respectively. Phosphodiesterase-1 (PDE-1) and transforming growth factor-β (TGF-β) expression in cardiac tissues was quantified using western blot and RT-PCR. Vinpocetine treatment and its combination with enalapril decreased glucose levels as compared to diabetic control rats. Vinpocetine improved echocardiographic parameters and cardiac functional status revealed by Biopac study. Vinpocetine decreased cardiac biochemical parameters, oxidative stress, inflammatory cytokines levels, cardiomyocyte diameters and fibrosis in rats. Interestingly, expressions of TGF-β and PDE-1 were ameliorated by vinpocetine alone and its combination with enalapril. Vinpocetine ameliorates diabetic cardiomyopathy by its anti-oxidant, anti-inflammatory, hypoglycaemic property and by inhibiting PDE-1 and TGF-β in rats.
Optimization on the jet characteristics of the main nozzle of hot-blowing snow removal device
Authors: Yu Qingrui , Hu Jinhai , Geng Yingsan , Zhang Xiangyi , Tang Yayun 
Published: July 2022

The exhaust devices used by snow removal vehicles are mainly based on hot-blowing snow removal. Due to the inherent characteristics of the turbojet engine such as low flow rate and high exhaust temperature, the modified hot-blowing snow removal device has problems of low snow removal efficiency and the possibility of ablation and damage to the road surface. In order to solve the problem, transforming the turbofan engine with a high flow rate and a low exhaust temperature into a hot-blowing snow removal device is an important improvement to achieve efficient and safe snow removal operations. In order to convert a medium bypass ratio turbofan engine into a hot-blowing snow removal device, the computational fluid dynamics (CFD) software CFX was used to analyze the internal and external flow field characteristics of the main nozzle of an exhaust device under the 60 % rated condition of the engine. The effective area sizes of the jet velocity and temperature predicted by the external flow field were used to optimize the layout of the main nozzle.
Marijuana Use and Health Outcomes in Persons Living With HIV: Protocol for the Marijuana Associated Planning and Long-term Effects (MAPLE) Longitudinal Cohort Study
Authors: Algarin Angel B , Plazarte Gabriela N , Sovich Kaitlin R , Seeger Stella D , Li Yancheng , Cohen Ronald A , Striley Catherine W , Goldberger Bruce A , Wang Yan , Somboonwit Charurut , Ibañez Gladys E , Spencer Emma C , Cook Robert L 
Published: July 2022

Background
Marijuana use is common in persons with HIV, but there is limited evidence of its relationship with potential health benefits or harms.

Objective
The Marijuana Associated Planning and Long-term Effects (MAPLE) study was designed to evaluate the impact of marijuana use on HIV-related health outcomes, cognitive function, and systemic inflammation.

Methods
The MAPLE study is a longitudinal cohort study of participants living with HIV who were recruited from 3 locations in Florida and were either current marijuana users or never regular marijuana users. At enrollment, participants completed questionnaires that included detailed marijuana use assessments, underwent interviewer-administered neurocognitive assessments, and provided blood and urine samples. Ongoing follow-ups included brief telephone assessments (every 3 months), detailed questionnaires (annually), repeated blood and urine samples (2 years), and linkage to medical records and statewide HIV surveillance data. Supplemental measures related to intracellular RNA, COVID-19, Alzheimer disease, and the gut microbiome were added after study initiation.

Results
The MAPLE study completed enrollment of 333 persons between 2018 and 2021. The majority of participants in the sample were ≥50 years of age (200/333, 60.1%), male (181/333, 54.4%), cisgender men (173/329, 52.6%), non-Hispanic Black (221/333, 66.4%), and self-reported marijuana users (260/333, 78.1%). Participant follow-up was completed in 2022, with annual updates to HIV surveillance data through at least 2027.

Conclusions
The MAPLE study is the largest cohort specifically designed to understand the use of marijuana and its effects on HIV-related outcomes. The study population has significant diversity across age, sex, gender, and race. The data will help clinicians and public health officials to better understand patterns of marijuana use associated with both positive and negative health outcomes, and may inform recommendations for future clinical trials related to medical marijuana and HIV.

International Registered Report Identifier (IRRID)
DERR1-10.2196/37153
The effect of venting structures on overpressure caused by an indoor explosion
Authors: Gorev V. A. , Korolchenko A. D. 
Published: July 2022

Introduction. The article considers an accidental indoor gas explosion on condition of pressure relief through openings in which venting structures were installed.A solution to this problem can protect residential buildings from consequences of explosions due to the fact that the volume of premises in residential buildings is small compared to industrial buildings, and it determines more stringent pressure relief conditions at the initial moment of the explosion development. The article shows that pressure can reach critical values in a small space during the motion of a venting structure in the opening before the onset of pressure relief.Goals. The authors aim to identify the pattern of blast load development from the moment of explosion to the attainment of the maximum pressure value with account taken of the properties of venting structures and patterns of their opening. This goal is relevant due to the fact that until now at this stage pressure development has been considered without any account taken of how deeply the venting structure is installed in the wall opening. Much attention was focused on the selection of the opening size.Methods. The methods of the theory of dimensions, numerical and analytical modeling of explosion processes, patterns of gas escape and rigid body motion were applied to obtain dimensionless groups describing the development of an explosive load until maximum values. These dimensionless groups allow identifying explosive loads for rooms having different volumes, which is also a new result.Results. In this work, the influence of individual factors on the ultimate result has been identified. These factors are the room volume, the pressure at which the venting structure starts moving, the mass and position of the venting structure in the opening, the opening perimeter and the rate of explosive combustion.Conclusions. The results, obtained in the course of this work, allow identifying the dynamic load of an explosion at the stage of its growth. This value can be used to set more reliable bearing characteristics of structures for cases of accidental explosions in living accommodations.