bluuebunny/crossref_metadata_2025_split huggingface dataset:
A case of infantile Pompe disease with intrauterine onset and literature review
Authors: Xi Hongmin , Li Xianghong , Ma Lili , Yin Xiangyun , Yang Ping , Zhang Lulu 
Published: July 2022

Abstract
Background: Pompe disease is a rare autosomal recessive disease.Acid alpha-glucosidase deficiency leads to glycogen storage in lysosomes, causing skeletal muscle, cardiac muscle and smooth muscle lesions.Pompe disease is progressive and the severity of the disease depends on the age of onset.The most severe form, known as classic infantile Pompe disease (IOPD), characterized by onset before 12 months.However, few cases of intrauterine onset have been reported.Case presentation: The proband was born at 40 weeks+3 days of gestational age and admitted to our hospital due to cardiac hypertrophy found in the uterus, shortness of breath and cyanosis after birth for 13 minutes. Physical examination at admission time showed poor response, pale skin, shortness of breath, low limb muscle tone and edema of both lower extremities. The heart sounds were weak without heart murmur. Echocardiography showed: left ventricular hypertrophy (9mm),right ventricular hypertrophy (5mm); The patient was given non-invasive ventilator-assisted respiration, fluid restriction, diuresis and metoprolol treatment. The child was diagnosed as infantile Pompe disease at 16 days with the acid alpha-glucosidase 0.31umol/l/h and the full penetrance gene test showing homozygous gene mutation c. 1844G>T(p.Gly615Val).The literature search retrieved a total of 3 articles and reported 5 Pompe disease infants with intrauterine onset. So there was a total of 6 cases,among whom,4 cases showing hypertrophic cardiomyopathy, 1 case with dilated cardiomyopathy, and 1 case had myocardial "mass". All cases had creatine kinase(CK) elevation, GAA decrease and gene mutation.2 cases needed ventilator assisted support and 4 cases were treated with enzyme replacement therapy(ERT), one of whom died at 14months; 2 patients without ERT treatment progressed rapidly, and our patient died at 7 months of age.Conclusion: Infants with intrauterine-onset Pompe disease mostly have early manifestations of heart disease. GAA determination and molecular genetic testing as soon as possible are helpful for parents to make early decision and early treatment.
LncRNA AOC4P impacts the differentiation of macrophages and T-lymphocyte by regulating the NF-κB pathways of KGN cells: potential pathogenesis of polycystic ovary syndrome
Authors: Wu Shujuan , Yang Zhe , He Shaojing , Han Lu , Zhou Mengqi , Yang Jing , Chen Jiao , Wu Gengxiang 
Published: July 2022

Abstract
Background
Polycystic ovary syndrome (PCOS) is a multifactorial endocrine disease, which is an important cause of female infertility worldwide. PCOS patients are in a state of chronic low-grade inflammation, and immune imbalance is considered as a potential cause of its pathogenesis.
Methods
The expression of AOC4P in PCOS and normal ovarian granulosa cells (GCs) was detected by real-time quantitative PCR. KGN cells were induced by dihydrotestosterone at 500 ng/ml to construct PCOS model. After lentivirus-infected, KGN cells were constructed with AOC4P overexpression cell lines, the proliferation and apoptosis levels of KGN cells in AOC4P and NC groups were detected. THP-1-derived macrophages and peripheral blood mononuclear cells (PBMC) were co-cultured with KGN cells for 48h respectively, and the differentiation of macrophages and CD4 + T cells were detected by flow cytometry.
Results
Decreased AOC4P expression was found in PCOS patients. After constructing PCOS cell model, we observed that overexpression of AOC4P promoted KGN cell proliferation and inhibited apoptosis. After co-culture with AOC4P overexpressed KGN cells, M1 macrophages decreased, M2 macrophages increased, Th1/Th2 ratio increased, and Treg cells increased. Finally, we found that AOC4P inhibited the activation of the nuclear factor κ B (NFκB) pathway in KGN cells.
Conclusions
In this study, we found that AOC4P regulated the NFκB signaling pathway by inhibiting the phosphorylation of P65, thereby affecting the proliferation and apoptosis of GCs, altering the differentiation of macrophages and T cells, and contributing to the pathogenesis of PCOS.
Summary of Research Progress on Separation and Extraction of Valuable Metals from Bayer Red Mud
Authors: Wang Kun , Dou Zhihe , Liu Yan , Li Xiaofei , Lv Guozhi , Zhang Ting-an 
Published: July 2022

Abstract
Bayer red mud is a strong alkaline solid waste discharged during alumina production. Due to large emissions and strong alkalinity, red mud is now mostly dammed or buried, which not only occupies huge land but also contaminates the surrounding ecosystem, causing the risk of collapse and landslide. In addition to its overall utilization in building materials, agriculture, the environment, and the chemical industry, red mud also contains valuable metals such as sodium, aluminum, iron, titanium, and scandium and is considered to be an important secondary resource. In this paper, the physicochemical properties and hazards of red mud are first introduced, and then the overall utilization of red mud is summarized. Then, the latest research progress on the separation and extraction of valuable metals from red mud is reviewed in detail, and a new comprehensive utilization method is proposed and analyzed. This paper also provides suggestions for the future development direction of the comprehensive utilization technology of red mud.
Diversity of the endophytic and rhizospheric fungal communities in Scrophularia ningpoensis roots and its correlation with active constituent contents
Authors: Ren Dan , Guo Kun yuan , Sun Qing mei , Zhu Bo , Qin Lu ping 
Published: July 2022

Abstract
Scrophularia ningpoensis, a perennial herb native to Southeast China, is used to prepare traditional Chinese medicines for the treatment of various diseases. The root microbiota play pivotal roles in providing hosts with nutrients, promoting growth, suppressing disease, and increasing abiotic stress tolerance. In this study, we investigated the composition of endophytic and rhizospheric fungi in the root microbiomes of S. ningpoensis obtained from different habitats and examined their potential effects on the active constituent contents of the host plant using high-throughput sequencing techniques. Our results indicated that 8709 operational taxonomic units (OTUs) and 5780 defined OTUs shared core rhizosphere OTUs. Ascomycota and Basidiomycota were dominant among the endophytic fungi, whereas Mortierellomycota was distributed in the rhizosphere. The fungal community in the rhizospheric compartment was more diverse than the endophytic community. Redundancy analysis and canonical correspondence analysis of the rhizospheric and endophytic samples revealed that the organic matter, total organic carbon, total nitrogen, and Hg levels were well-correlated with the composition of rhizospheric and endophytic fungal communities. Multiple linear regression analyses facilitated the identification of potentially beneficial fungi whose abundance was correlated with active constituent levels. These fungi could potentially provide valuable information regarding the use of S. ningpoensis in the medicinal plant industry.
Adaptive Regularized Zero-Forcing Beamforming in Massive MIMO with Multi-Antenna Users
Authors: Bobrov Evgeny , Chinyaev Boris , Kuznetsov Viktor , Lu Hao , Minenkov Dmitry , Troshin Sergey , Yudakov Daniil , Zaev Danila 
Published: July 2022

Abstract
Modern wireless cellular networks use massive multiple-input multiple-output (MIMO) technology. This technology involves operations with an antenna array at a base station that simultaneously serves multiple mobile devices which also use multiple antennas on their side. For this, various precoding and detection techniques are used, allowing each user to receive the signal intended for him from the base station. There is an important class of linear precoding called Regularized Zero-Forcing (RZF). In this work, we propose Adaptive RZF (ARZF) with a special kind of regularization matrix with different coefficients for each layer of multi-antenna users. These regularization coefficients are defined by explicit formulas based on SVD decompositions of user channel matrices. We study the optimization problem, which is solved by the proposed algorithm, with the connection to other possible problem statements. We also compare the proposed algorithm with state-of-the-art linear precoding algorithms on simulations with the Quadriga channel model. The proposed approach provides a significant increase in quality with the same computation time as in the reference methods.
Finite Element Method-enhanced Neural Network for Forward and Inverse Problems
Authors: Meethal Rishith Ellath , Kodakkal Anoop , Khalil Mohamed , Ghantasala Aditya , Obst Birgit , Bletzinger Kai-Uwe , Wüchner Roland 
Published: July 2022

Abstract
We introduce a novel hybrid methodology that combines classical finite element methods (FEM) with neural networks to create a well-performing and generalizable surrogate model for forward and inverse problems. The residual from finite element methods and custom loss functions from neural networks are merged to form the algorithm. The Finite Element Method-enhanced Neural Network hybrid model (FEM-NN hybrid) is data-efficient and physics-conforming. The proposed methodology can be used for surrogate models in real-time simulation, uncertainty quantification, and optimization in the case of forward problems. It can be used to update models for inverse problems. The method is demonstrated with examples and the accuracy of the results and performance is compared to the conventional way of network training and the classical finite element method. An application of the forward-solving algorithm is demonstrated for the uncertainty quantification of wind effects on a high-rise buildings. The inverse algorithm is demonstrated in the speed-dependent bearing coefficient identification of fluid bearings. Hybrid methodology of this kind will serve as a paradigm shift in the simulation methods currently used.
Epidemiological study of HPV infection in 40693 women in Putian: a population study based on screening for high-risk HPV infection
Authors: Chen Zhanfei , Lin Hua , Zheng Jinyuan , Chen Zhonghui , Li Jinqiu , Yu Liumin , Cai Lili 
Published: July 2022

Abstract
Background: The infection rate of human papillomavirus (HPV) is high in coastal regions of China. However, the infection of high-risk genotypes of women in Putian City is unknown. Therefore, this study aimed to analyse the epidemiology of high-risk HPV infection among women in Putian and provide a reference for the diagnosis, treatment, and vaccination of cervical cancer in this region.Methods: A total of 40693 female cervical cell exfoliation samples screened for high-risk HPV at the Affiliated Hospital of Putian University from July 2020 to December 2021 were selected. DNA was extracted using a fully automatic extractor. Then, 14 high-risk genotypes of HPV were detected by polymerase chain reaction. The characteristics of HPV infection, distribution of high-risk genotypes, infection types, and TCT classification in different age groups were analysed.Results: Of the 40693 samples, 3899 were infected with HPV, with an infection rate of 9.6%. Accordingly, HPV infection rates gradually increased with age, with statistically significant differences among age groups (χ2=74.03, P<0.01). The infection rates of high-risk HPV52, HPV58, and HPV16 ranked in the top three and increased with age. Single infection was dominant (84.7%), followed by double infections (12.7%). In addition, the cervical cytological classification of 3899 HPV-positive people was as follows: NILM (88.0%), ASC-US (6.6%), ASC-H (1.4%), LSIL (3.2%), and HSIL (0.8%). The study found that the HPV16 infection rate increased with increasing severity of cervical cytology (χ2trend=43.64, P<0.01), while the infection rates of HPV52 (χ2trend=13.89, P<0.01) and HPV58 (χ2trend=13.50, P<0.01) showed opposite trends.Conclusions: The infection rate of female HPV high-risk screening in this region was 9.6%, mainly for single infections. In addition, HPV16, HPV52, and HPV58 were closely related to the severity of cervical cytology. When vaccinated against HPV, the nine-valent vaccine is more effective in preventing infection. Health departments and medical institutions need to strengthen regular HPV screening and science publicity for young and middle-aged women, since both are crucial for preventing cervical cancer.
Determination of MeHg and EtHg in Soils and Sediments with Purge and Trap/Gas Chromatography Cold Atomic Fluorescence Spectrometry
Authors: Zhang Linlin , Wang Qing , Lv Jungang 
Published: July 2022

Abstract
Mercury and its compounds, especially methyl mercury(MeHg) and ethyl mercury(EtHg), are ubiquitous in the environment. It is necessary to the determinate mercury species due to their high toxicity and biomagnification. A novel method is described for the Hg analysis in soil and sediment samples in this study. Samples were extracted with alkaline(KOH/CH3OH), MeHg and EtHg in the extraction solution are derived by sodium tetrapropyl boride to generate volatile methylpropyl mercury and ethylpropyl mercury. After purging and trapping, thermal desorption and gas chromatography separation, Hg compounds were cracked into mercury vapor at high temperature,and finally detected by atomic fluorescence spectrometer, qualitative according to retention time and quantitative with external standard method. Pretreatment and instrumental parameters were optimized in detail. The recoveries of MeHg and EtHg were 83.0%-109% and 90.3%- 96.5%, respectively. The recoveries of the certified standard materials were higher than 90.0%,and the relative standard deviation were less than 1.0%. The method was finally applied to the real samples of black, red and fluvo-aquic soils. The recoveries of MeHg and EtHg were 85.1%-109% and 90.3%-96.3% with the RSDs less than 6.0%. This method was fast and accurate, which could be applied to the analysis of large quantities of samples. After properly adjusting the pretreatment method, this method could also be applied to the analysis of alkyl mercury in environmental organisms (such as fish, shellfish), atmospheric particles and other samples, which hinted its wide application range and promising application prospect.
pH responsive Fe3O4@GT/PVA nanocomposite for drug release of hydroxychloroquine sulfate (HCQ)
Authors: Zandi Ali , Ahangar Hossein Abbastabar , Saffar Ali 
Published: July 2022

Abstract
Recent studies have shown the potential of hydroxychloroquine (HCQ) as a pharmaceutical agent to the treatment of a variety of cancers such as lung, melanoma, breast, pancreatic, and colon cancers. HCQ has been proposed to cure viral diseases such as Covid-19 disease. In this study, Fe3O4@PVA/GT nanocomposite has been synthesized for controlling HCQ release at pH 7.4 and 3.4 in different temperature (37 0C and 40 0C). Taguchi method (L9) with 3 levels and 3 factors was used to optimize the influence of the ratio of PVA (polyvinyl alcohol) to GT (gum tragacanth), Fe3O4 weight percent, and the amount of crosslinking agent (citric acid) on the swelling and releasing rate. The influence of different factors were studied on the swelling and releasing rate such as the ratio of PVA (polyvinyl alcohol) to GT (gum tragacanth), the amount of Fe3O4 and the amount of crosslinking agent (citric acid), by the Taguchi method with 3 factors at 3 levels. The obtained results showed that increasing the amount of Fe3O4 decreased the drug release rate. Increasing the amount of GT to PVA increased the sensitivity of the nanocomposite to pH. So that in neutral pH had the lowest drug release and in acidic pH had the highest drug release. After 18 hours, sample with PVA / GT ratio 0.85:0.15 (wt/wt), CA 3% (wt) and 2% (wt) Fe3O4 had the cumulative drug release 71.57 % and 52.37% in pH 3.4 and 7.4 at 40 0C, respectively.
Simultaneously enhancing the strength and ductility of medium-entropy alloys via manipulating ordered oxygen complexes
Authors: Jiao Meiyuan , Lei Zhifeng , Wu Yuan , Du Jinlong , Li Wenyue , Zhu Xiangyu , Yuan Xiaoyuan , Wang Shudao , Zhu Huihui , Cao Peipei , Liu Xiongjun , Wang Hui , Jiang Suihe , Lu Zhaoping 
Published: July 2022

Abstract
Oxygen solute strengthening is an effective strategy to harden metallic materials, yet, it usually deteriorates the ductility. Ordered oxygen complexes, a state between regular random interstitials and oxides, can simultaneously enhance strength and ductility in high-entropy alloys. However, if such strengthening media can be created and if this particular strengthening mechanism still holds in other alloys remain unclear. Here, we demonstrated that OOCs can be obtained in the single bcc (body-centered-cubic) Ti-Zr-Nb medium-entropy alloys (MEAs) via tailoring the content of bcc-stabilizer Nb and oxygen. It was found that decreasing the bcc phase stability (i.e., lowering the Nb content) enhances the degree of chemical short-range orderings, particularly those enriched in Ti and Zr, and then favors the formation of ordered complexes after doping oxygen. Moreover, the number density of OOCs increases with oxygen contents (\(\le 3.0\)at.%) in a given alloy, but addition of excessive oxygen (\(>3.0\)at.%) leads to grain boundary segregation. As a result, the tensile yield strength is appreciably enhanced by ~ 75% and ductility is substantially improved by ~ 164% when doping the Ti-30Zr-14Nb MEA with 3.0 at.% oxygen. Our work not only manifests the universality of the novel interstitial strengthening mechanism, but also offers insights into developing advanced metallic materials with localized atomic orderings.
Valeric Acid acts as a novel HDAC3 inhibitor against Prostate Cancer
Authors: Han Rui , Yang Hongxing , Li Ya , Ling Changquan , Lu Lingeng 
Published: July 2022

Abstract
Prostate cance is the second cause of cancer-related deaths in men worldwide, and new agents for curing the disease are still needed. In this study, we theorectically and experimentally demonstrated that valeric acid (VA) was a HDAC inhibitor, anticancer efficacy of VA in prostate cancer cells was also observed using either 2D or 3D culture systems. VA was cytotoxic for prostate cancer cells but low-toxic to normal cells. VA significantly inhibited E2F1/E2F3 expression but increased CASP3 activity. In vivo mouse models further showed its anticancer activity and potential property of chemosensitizer with promoting apoptosis. The findings suggest that VA acts as a HDAC3 inhibitor with anti-cancer effect on prostate cancer by regulating E2F1/E2F3/CASP3 axis.
Emotional dysregulation in endometriosis women with cyclical and non-cyclical chronic pelvic pain
Authors: Rodríguez-Lozano Dulce Carolina , Meza-Rodríguez María del Pilar , Cruz-Orozco Olivier Paul , Sánchez-Ramírez Brenda , Olguin-Ortega Andrea , Silvestri-Tomassoni José Roberto , Corona-Barsse Guillermo , Escobar-Ponce Luis Fernando , Solis-Paredes Juan Mario , Dominguez-Trejo Benjamín , Camacho-Arroyo Ignacio 
Published: July 2022

Abstract
Background: Endometriosis is a pathophysiological condition characterized by glands and stroma outside the uterus in regions such as the bladder, ureter, fallopian tubes, peritoneum, ovaries, and even in extra pelvic sites. One of the main clinical problems of endometriosis is chronic pelvic pain (CPP), which considerably affects the patients’ quality of life. Patients with endometriosis may, cyclically or non-cyclically (80% of cases) experience CPP. High levels of anxiety and depression have been described in patients with endometriosis related to CPP; however, this has not been evaluated in endometriosis women with different types of CPP. Methods: This work aimed to determine if there are differences in emotional dysregulation in patients with cyclical and non-cyclical CPP. 49 women diagnosed with endometriosis presenting cyclical and non-cyclical CPP answered several batteries made up of Mini-Mental State Examination, Visual Analog Scale, Beck´s Depression Inventory, State Trait-Anxiety Inventory, and Generalized Anxiety Inventory. Results: We observed that patients with non-cyclical CPP exhibited higher levels of depression and anxiety (trait-state and generalized anxiety) than patients with cyclical pain. No differences were observed in pain intensity, but there was a higher probability of developing emotional dysregulation (anxiety or depression) in patients with non-cyclical CPP. No differences were observed in cognitive impairment. Conclusions:Our data suggest that patients with non-cyclical CPP present a higher emotional dysregulation than those with cyclical pain.
Machine learning for prediction of bronchopulmonary dysplasia-free survival among very preterm infants
Authors: Leigh Rebekah M. , Pham Andrew , Rao Srinandini S. , Vora Farha M. , Hou Gina , Kent Chelsea , Rodriguez Abigail , Narang Arvind , Tan John B. C. , Chou Fu-Sheng 
Published: July 2022

Abstract
Background
Bronchopulmonary dysplasia (BPD) is one of the most common and serious sequelae of prematurity. Prompt diagnosis using prediction tools is crucial for early intervention and prevention of further adverse effects. This study aims to develop a machine learning-based BPD-free survival prediction tool based on the concept of the developmental origin of BPD.
Methods
Datasets comprising perinatal factors and early postnatal respiratory support were used for initial model development, followed by combining the two models into a final ensemble model using logistic regression. Simulation of clinical scenarios was performed.
Results
Data from 689 infants were included in the study. We randomly selected 80% of which was used for model development and used the remainder 20% for validation. The performance of the final model was assessed by receiver operating characteristics which showed 0.921 (95% CI: 0.899–0.943) and 0.899 (95% CI: 0.848–0.949) for the training and the validation datasets, respectively. Simulation data suggests that extubating to CPAP is superior to NIPPV in BPD-free survival. Additionally, successful extubation may be defined as no reintubation for 9 days following initial extubation.
Conclusions
Machine learning-based BPD prediction based on perinatal features and respiratory data may have clinical applicability to promote early targeted intervention in high-risk infants.