bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility Study And Surgical Technique Sharing Of Myomectomy For Intramural Myoma Greater Than 8cm During Cesarean Section
Authors: Shi Chunbo , Chen Aner , Chen Jinliang 
Published: March 2022

Abstract
Objective: To explore the feasibility and safety of cesarean myomectomy in large interstitial myoma.Methods: A total of 124 cases of pregnancy who underwent cesarean section in Ningbo Women's and Children's Hospital were selected. 79 patients were complicated with intramural uterine leiomyoma, and 43 patients underwent cesarean myomectomy (Study group), and 36 patients did not undergo myomectomy during cesarean section (regular group). No uterine leiomyoma was found in the other 45 patients during cesarean section (control group). The intraoperative conditions were compared, including operation time, intraoperative blood loss and uterine balloon packing rate. The short-term recovery after operation was compared, including the time of use of antibiotics, postoperative hospital stay, postoperative fever rate and anal exhaust time. Long-term recovery after discharge was followed up by telephone. Results: Compared with the operation time of the three groups, the operation time of the study group was significantly longer than that of the regular group and the control group[75(60, 150) min vs 69.5(40, 100) min vs 58(41, 70) min, P<0.001]. In the control group, the study group and the regular group, the intraoperative blood loss was 400（350，600）ml vs 600（400,2500）ml vs 550（400,1300）ml, P <0.001], antibiotic use time [2(1,3) days vs 3(2,5) days vs 3 (2,12) days, P <0.001], postoperative hospital stay [4(3,5) days vs 5(4,6) days vs 5(4,14) days, P <0.001], anal exhaust time [1(1,1) day vs 1(1,5) day vs 1(1,3) day, P <0.05], but there was no significant difference between the study group and the regular group (P > 0.05). Compared with the study group and the control group, the balloon implantation rate of the regular group was the highes (33.3% vs 11.65% vs 0%, P < 0.05). There was no significant difference in the incidence of postoperative fever (>38℃) among the three groups.Conclusions: For pregnancy complicated with large uterine leiomyoma, it is safe and feasible to be resected by experienced obstetricians and gynaecologists at the same time of cesarean section.
Structural insights into RyR channel gating modulated by intrinsic junctional protein
Authors: Hu Hongli , Wei Risheng , Chen Qiang , Zhang Lei , Liu Congcong , Zhang Mengxue , Liu Chuang , Yin Chang-Cheng 
Published: March 2022

Abstract
The type-1 ryanodine receptor (RyR1) is an intracellular calcium release channel for skeletal muscle excitation-contraction coupling. Published structures of RyR1 showed RyR1 is open only in the assistance of exogenous regulators, such as caffeine, ryanodine, PCB-95 and diamide. Here, we report that with a mild purification procedure, a single transmembrane protein junctin is co-purified with RyR1. RyR-junctin complex can be activated to open state by Ca2+ only. Junctin inserts its transmembrane helix next to S1, S4 and S4-5 linker of RyR1, exerting an out-ward pushing force to the TM domain, facilitating the dilation of S6. Junctin regulates the channel conformation by directly affecting the TM domain via Jun/S1-4–S4-5 linker. Additional ATP increases the channel open probability by further stabilizing the channel in the open state. Our results demonstrate junctin forms an intrinsic complex with RyR1 and play a key role in the channel gating of RyR1 in physiological context.
Comparison of sitting position vs. non sitting position the resection of brain metastases in the posterior fossa. Surgical and functional outcome in a contemporary cohort.
Authors: Krauss Philipp , Vernik Tamara , Motov Stefan , Bonk Maximilian Niklas , Shmygalev Sergey , Kramer Katharina , Lehmberg Jens , Shiban Ehab 
Published: March 2022

Abstract
For surgery of brain metastases, good immediate postoperative functional outcome is of utmost importance. Improved functional status can enable further oncologic therapies and adverse events might delay them. Pros and cons of either sitting or supine positioning for resective surgery of the posterior fossa are debated but contemporary data on direct postoperative outcome is rare. Aim of our study was to compare the functional outcome and adverse events of surgery for brain metastases in sitting vs. non sitting position in the direct postoperative setting. We retrospectively compared surgery of metastases located in the posterior fossa over a 3-year period in two level-A neurosurgical centers. Either center performed surgery exclusively in sitting or non-sitting positioning respectively. Worse functional outcome (Karnofsky Performance scale) and functional deterioration was seen in the “sitting” group (coming from higher functional scores). We found significantly more “sitting” patients to deteriorate to a KPS ≤60% including four deaths (vs. one in non-sitting position). In this study, treating patients with brain metastases in sitting position resulted in a number needed to harm (NNH) of 2.3. In this study, we found sitting position for surgery of brain metastases to be associated with worse outcome and more adverse events. Therefore, we tend to recommend non-sitting over sitting position for surgery of brain metastases of the posterior fossa.
“In the beginning, I said I wouldn’t get it.”: In-depth Qualitative Interviews to Understand Vaccine Hesitancy, Acceptance, and Decision-Making in Remote Alaska between November 2020 and July 2021
Authors: Eichelberger Laura P. , Fried Ruby L. , Cochran Patricia , Hahn Micah 
Published: March 2022

Abstract
Background
Despite leading national rates early in the pandemic, COVID-19 vaccine coverage in Alaska currently ranks 26th nationwide. Prevailing literature primarily reports urban or aggregated data. Here instead, we further characterize the complex decision-making around the COVID-19 vaccine based on in-depth interviews with Alaskans living off the road system.
Methods
We conducted 38 semi-structured in-depth interviews by phone with remote Alaska residents. Interviewees were survey respondents who had self-selected for a follow-up interview, and were predominately Indigenous, female, with post-secondary education. Interviews occurred in February and July 2021, after emergency use COVID-19 vaccine approval.
Results
Of 38 interviewees, 16 (42%) were “low intention” or “deliberating” vaccine acceptance based on their survey responses. Of these, 13 (81% of low-intention/deliberating) got vaccinated between the survey and follow-up interview timeframes. Vaccinated interviewees were motivated by individual-level perceptions of benefits (n = 18, 82%), protecting others (n = 6, 27%), and current or potential requirements (n = 7, 32%). Over half (n = 22, 58%) had vaccine-related concerns, including 13 (46%) of those vaccinated. Concerns primarily pertained to safety (n = 18, 47%), especially potential side effects (n = 11, 29%). Interviewees reported using multiple sources to make their decision, including media (n = 18, 49%) and interpersonal interactions (n = 16, 42%). Confidence among vaccinated/high-intention interviewees was attributed to trusting the source and/or information (n = 14, 44%), learning from others (n = 9, 28%), and personal experiences (n = 8, 25%). Scientific knowledge does not appear to have been a dominant factor for decision-making. Unvaccinated/low-intention interviewees (n = 5) desired more time and information.
Conclusions
This paper illustrates how vaccine hesitancy and acceptance are dynamic decisions that are influenced by complex processes involving knowledge circulation and reception. These results show that even those who respond that they will “definitely not” get vaccinated may change their minds at some future point. Further, vaccinated individuals may still have vaccine-related concerns. These findings indicate that building vaccine confidence involves a combination of consistent messages disseminated through a variety of media, as well as interpersonal interactions and observations. Trusting the source and/or understanding the information, as well as having a sense of agency appears to be important to interviewee decision-making, regardless of vaccination status and intention.
Primary Tumor Size as a Predictor of Prognosis in Patients with Stage IV Colorectal Cancer: Population analysis based on two centers
Authors: Chen Junguo , Chen Xijie , Lv Min-Yi , Zhang Bin , Li Guanman , Lin Yanyun , Huang Tianze , He Degao , Hu Jiancong , He Xiaosheng 
Published: March 2022

Abstract
Background
The prognostic significance of primary tumor size in patients with stage IV colorectal cancer (CRC) has not been established. This study aimed to evaluate the prognostic value of tumor size in stage IV CRC.
Methods
We retrospectively analyzed the data of patients with stage IV CRC in the Surveillance, Epidemiology and End Results (SEER) database and patients with stage IV CRC treated at the Sixth Affiliated Hospital of Sun Yat-sen University (SYSU). The optimal cutoff value for tumor size was determined using the X-Tile program. Multivariable Cox regression analysis were used to examine whether tumor size was an independent predictor of prognosis.
Results
There were 16,283 patients in the SEER cohort and 462 in the SYSU cohort. With the help of the X-Tile program, we selected 60mm as the optimal tumor size cutoff. Multivariate analysis confirmed that tumor size (HR = 1.17, 95%CI: 1.13–1.22) was an independent prognostic factor in stage IV CRC. Across all subgroups in the SEER cohort, survival probability was significantly lower in patients with tumor size ≥ 60 mm than in patients with tumor size < 60 mm.
Conclusions
Tumor size appears to be an independent predictor of survival in patients with stage IV CRC. Patients with tumor size ≥ 60 mm have poor prognosis.
Calculation Of WER And WET For 3 Dosimetric Materials At Carbon Ion Energies Ranging 100-300 Mev Using Fluka And MCNPX Monte Carlo Codes
Authors: Yjjou M. , Dekhissi H. , Derkaoui J. , Didi A. , Aknouch A. 
Published: March 2022

Abstract
Due to the shortage of data regarding range, WER and WET values for carbon ion beams in a vast range of energies as well as for relevant dosimetric materials, in this work, firstly, depth-dose profiles and range were evaluated in liquid water, polyethylene (PE), aluminium, bone, polymethyl methacrylate (PMMA), and lead for carbon ion energies ranging 100-300 MeV using Fluka Mc code. Afterwards, following their most frequent use in dosimetric studies in hadrontherapy, we decided to calculate the WER and WET of only 3 dosimetric materials (PE, PMMA and lead). Then, we made the remark that the PE and the lead represent the least and the most difference to water with calculated mean WER values of 0.998 and 5.684, respectively. The calculated WER values were compared with MCNPX Mc code as well as the previous analytical results (NM, BK, BB, EBB). Good agreement was reached between Fluka, MCNPX and the previous analytical results. The largest discrepancy between the two Mc codes was 2.29% for lead at 250 MeV carbon ion energy and was about 2.06% between Fluka and the BB method for Lead at 270.83 MeV carbon ion energy. Based on the materials explored, the PE stayed the nearest to water with an average WER of 0.9983 for carbon ion energy ranging 100 to 300 MeV. KeysWords: WER, WET, carbon ion, hadrontherapy, Fluka, MCNPX
The Protective Role of Exosomal circRNA-0008302 from Adipose-Derived Stem Cells (ADSCs) Against Myocardial Injury
Authors: Hu Chengyan , Sun Xipeng , Chu Yanyan , Wang Yanling , Liu Zhi 
Published: March 2022

Abstract
Background
This study aims to explore the expression of circRNA-0008302 in adipose stem cells derived exosome (ADSCs-Exo), and to investigate the function as well as mechanism of circRNA-0008302 in H2O2-induced cardiomyocyte injury.
Methods
Differentially expressed circRNAs between ADSCs and ADSCs-Exo were identified by circRNA chip screening, and further validated by qRT-PCR. CircRNA-0008302 siRNA was used to silence circRNA-0008302 expression in M6200 mouse cardiac myocytes. M6200 cells were divided into four groups: M6200 group, M6200 + H2O2 group, M6200 + H2O2 + ADSCs-Exo group and M6200 + H2O2 + ADSCs-Exocirc−0008302 siRNA group. Thereafter, cell viability was evaluated using CCK8 assay. Apoptosis and intracellular ROS levels were measured by flow cytometry. The expression level of miR-466i-5p was evaluated by qRT-PCR. Western blotting was performed to detect MsrA protein expression.
Results
The expression of circ-0008302 was significantly increased in ADSCs-Exo compared with that in ADSCs. Through the delivery of circ-0008302, ADSCs-Exo maintained the viability, inhibited the apoptosis and reduced ROS production in cardiomyocytes, thereby protecting cardiomyocytes from H2O2-induced oxidative injury. Mechanistically, circ-0008302 downregulated the level of miR-466i-5p, and thus promoted the expression of a miR-466i-5p target gene-MsrA in cardiomyocytes.
Conclusion
ADSC-secreted exosomes have a protective role in myocardial injury by delivering circ-0008302, which increased MsrA expression via targeting miR-466i-5p in cardiomyocytes.
Prevalence and correlates of major depressive disorders among patients with cancer at Kamuzu Central Hospital in Malawi
Authors: Banda Jonathan Chiwanda , Udedi Michael , Masamba Leo , Liwimbi Olive , Zimba Chifundo Colleta , Kamzati Moses , Mphonda Steve , Muula Adamson Sinjani 
Published: March 2022

Abstract
Background
Depressive disorders, with prevalence varying widely by setting, are among the common debilitating mental health disorders in patients with cancer worldwide. An understanding of the prevalence of depressive disorders among a specific patient population group should inform both their prevention and management. We conducted this study to estimate the prevalence and correlates of depressive disorders among patients living with cancer at Kamuzu Central Hospital (KCH), Lilongwe in Malawi.
Methods
We conducted a cross-sectional study nested in another larger quasi-experimental study evaluating the effect of integrating depression screening and management on clinical outcomes among cancer patients attending oncology services at a Cancer Unit based at Kamuzu Central Hospital in Lilongwe. A validated PHQ-9 tool was used to estimate prevalence of major depressive disorders. A total of 399 consented participants were enrolled from August to December, 2021. We estimated the prevalence and associated factors to depression among this patient group.
Results
The study comprised more females (64%) than males. Cervical cancer (33%), Kaposi’s Sarcoma (16%), breast cancer (9%) and esophageal cancer (4%) were the top four common malignancies in the study. The prevalence of major depressive disorders was 11.5%; most (52%) of whom had moderate depressive symptoms on PHQ-9 scale. Patients receiving palliative care were associated with reduced odds of major depressive disorders [Odds ratio: 0.36 (95% CI: 0.14–0.94)].
Conclusion
A high proportion of patients with cancer had major depressive disorders. Patients on palliative care were protected from having major depressive disorders. There is a need for integrating screening and treatment of depressive disorders among patients living with cancer to improve cancer care in Malawi.
iEngage: a digital health education program designed to enhance physical activity in young adolescents
Authors: CAILLAUD Corinne , LEDGER Susan , Diaz Claudio , CLERC Gael , GALY Olivier , YACEF Kalina 
Published: March 2022

Abstract

iEngage© is a modular health education and behavioural change program designed to provide adolescents with knowledge and practical skills and to help them moderate to vigorous physical activity (MVPA). Key features of the program include the iEngage© app which integrates learning modules with activity trackers data (Misfit Ray©), evidence-based health information, guidance through goals setting, self-monitoring, assessment of achievements, and experiential learning supported by the activity trackers. iEngage© was implemented in school context in 10–12 years old adolescents over 5 weeks involving 10 learning and behavioural change modules and continuous monitoring of steps. Results show that adolescents effectively set goals and self-assessed achievements during the program, progressing toward higher activity levels. Daily steps increased through the program (+ 30% or + 2647 steps/day) as well as the consistency of days totalling at least 11,000 steps/day (from 35% at the beginning to 48% at the end of the program). Average schooldays MVPA was assessed pre- and post- via research grade GENEActiv wrist accelerometers in iEngage and control participants. Contrasting with the control group, MVPA increased after the program ( ~ + 5 min/day) in short bouts, particularly during lunch time, recess and after school. Girls were found less active than boys and progressed goals and PA achievements less than boys during the program. The iEngage© program and its digital app, incorporates experiential learning, goals setting, self-monitoring and self-assessment of PA, and effectively helped young adolescents enhance PA and build up MVPA in daily life.
Comprehensive analysis of a ceRNA network associated with TP53 mutations in liver hepatocellular carcinoma
Authors: Yuan Jie , Xu Zongren , Gan Hongyan , Li Ruiqian , Jia Junhao , Yu Zicheng , Peng Jing 
Published: March 2022

Abstract
Liver cancer is one of the most common causes of cancer-related death and is quantitatively dominated by the liver hepatocellular carcinoma (HCC) subtype. Although advances have been made in recent years, further exploration on new HCC carcinogenic mechanisms as well as diagnostic and therapeutic methods in the context of detrimental TP53 abnormalities is indispensable on the way to conquer HCC. In this study, competing endogenous RNA (ceRNA) network was constructed on tumor samples stratified by the TP53 mutational status. With demonstrated TP53 mutant-specificity, the network nodes including AC017104.6, RP5-1092A11.5, RP11-365O16.6 and PTBP1 were all prognosis-related. Further therapeutic correlation analysis unveiled the connection between their expressions and tumor microenvironment fluctuation as well as possible chemotherapy resistance. Additionally, we explored the drug sensitivity difference between TP53 wild type and mutant samples, complementing the therapeutic arsenal of HCC. The universality of the key node PTBP1 was verified by multi-source transcriptional and protein level expressions. Taken together, this is the first study focusing on the ceRNA network mediated by TP53 mutations and its provoked therapeutic associations in HCC. Our results deepened the understanding of the concomitant alternations brought by TP53 mutations and could benefit future therapeutic development in HCC.
Inhibitors of Aβ Peptide Aggregation as Potential Anti-Aizheimer Agents
Authors: Bandiera Tiziano , Lansen Jacqueline , Post Claes , Varasi Mario 
Published: March 2022

Alzheimer's disease (AD), the most common form of dementia in elderly people, is characterized, from the pathological standpoint, by the extracellular deposition of a 39-43 amino acid peptide, referred to as the amyloid -peptide (A ), in the form of insoluble, protease resistant amyloid plaques. Apeptides are known to aggregate spontaneously in vitro with the formation of amyloid fibrils. A great body of evidence has accumulated on the dependence of Atoxicity on its aggregation state: on this basis, the hypothesis has been put forward that the deposition of amyloid plaques is a causative factor in AD.	Therefore, the intervention on the Apeptide aggregation process can be envisaged as an approach to stop or, at least, to slow down the progression of AD. In the last few years, a number of small molecules have been reported to interfere with the in vitro aggregation of Apeptides. -cyclodextrin and rifampicin are the first compounds that were shown to inhibit fibril formation by Apeptides; Congo Red, chrysamine G and other sulfonated dyes were found to display the same activity and, recently, nicotine and iododoxorubicin were reported to act as aggregation inhibitors as well. Although most of these molecules can only be considered as tools, they should be regarded as structural hits in the search for more active and pharmacologically useful compounds. The available data on these compounds are reported in this review.
Normalizing Telemonitoring in Nurse-Led Care Models for Complex Chronic Patient Populations: Case Study
Authors: Gordon Kayleigh , Dainty Katie N , Steele Gray Carolyn , DeLacy Jane , Shah Amika , Seto Emily 
Published: March 2022

Background
The implementation of telemonitoring (TM) has been successful in terms of the overall feasibility and adoption in single disease care models. However, a lack of available research focused on nurse-led implementations of TM that targets patients with multiple and complex chronic conditions (CCC) hinders the scale and spread to these patient populations. In particular, little is known about the clinical perspective on the implementation of TM for patients with CCC in outpatient care.

Objective
This study aims to better understand the perspective of the clinical team (both frontline clinicians and those in administrative positions) on the implementation and normalization of TM for complex patients in a nurse-led clinic model.

Methods
A pragmatic, 6-month implementation study was conducted to embed multicondition TM, including heart failure, hypertension, and diabetes, into an integrated nurse-led model of care. Throughout the study, clinical team members were observed, and a chart review was conducted of the care provided during this time. At the end of the study, clinical team members participated in qualitative interviews and completed the adapted Normalization Measure Development questionnaires. The Normalization Process Theory guided the deductive data analysis.

Results
Overall, 9 team members participated in the study as part of a larger feasibility study of the TM program, of which 26 patients were enrolled. Team members had a shared understanding of the purpose and value of TM as an intervention embedded within their practice to meet the diverse needs of their patients with CCC. TM aligned well with existing chronic care practices in several ways, yet it changed the process of care delivery (ie, interactional workability subconstruct). Effective TM normalization in nurse-led care requires rethinking of clinical workflows to incorporate TM, relationship development between the clinicians and their patients, communication with the interdisciplinary team, and frequent clinical care oversight. This was captured well through the subconstructs of skill set workability, relational integration, and contextual integration of the Normalization Process Theory.

Conclusions
Clinicians successfully adopted TM into their everyday practice such that some providers felt their role would be significantly and negatively affected without TM. This study demonstrated that smartphone-based TM systems complemented the routine and challenging clinical work caring for patients with CCC in an integrated nurse-led care model.