bluuebunny/crossref_metadata_2025_split huggingface dataset:
USPIO combined with MR to long-term show the stem cells to repair the defect of articular cartilage
Authors: Jing Qing , Zhao Jingjing , Li Tongtong , Shi Wenjing , Xu Yang , Wang Wenhui , Zhu Jiahong , Liu Zhenyu , Qi Meiling , Zhen Junping 
Published: December 2022

Abstract
Backgroud:
Magnetic Resonance Imaging (MRI) is used to monitor the process of repairing articular cartilage defect by transplanting the bone marrow mesenchymal stem cell marked by Ultrafine Superparamagnetic Iron Oxide (USPIO) in the rabbit.
Methods
The Bone Marrow Stromal Cells (BMSCs) cells are isolated from rabbits injected with USPIO (20 mg/kg). Staining and cck-8 are used to detect the ability to form bone, fat cartilage. In the 4 rabbit groups of the double knee joint cartilage defect, the 3 groups are respectively injected the BMSCs-sodium alginate gel compound marked USPIO, BMSCs-sodium alginate gel compound, pure sodium alginate gel, while the blank group doesn't do anything. The rabbits are assessed by MRI and histological studying.
Results
Compared with the control group, the BMSCs marked by USPIO are the same in cell morphology. The proliferative and differentiation ability of BMSCs in rabbits is not affected by USPIO with a concentration of 20mg/kg. We find that the cartilage defects are gradually repaired in the two cell transplantation groups, and it is slowly repaired by BMSCs transplanting for the treatment of rabbit knee cartilage defects in the other groups. The labeled BMSCs could be used to treat the rabbit knee cartilage defect but could be indicated by MRI.
Conclusion
The experimental results show that the BMSCs marked by USPIO are the same in cell morphology compared with the control group.
Molecular docking studies on the inhibitory selectivity of cytochrome P450 2C9 by natural anti-arthritic compounds
Authors: Tan Boon Hooi , Ahemad Nafees , Pan Yan , Palanisamy Uma Devi , Othman Iekhsan , Yiap Beow Chin , Ong Chin Eng 
Published: December 2022

Abstract
Five natural anti-arthritic compounds, diacerein, rhein, glucosamines (glucosamine 3-sulfate, G3S, and glucosamine 6-sulfate, G6S), and chondroitin disaccharide Δdi-4S (C4S) were docked individually to the defined binding site in CYP2C9 based on published crystal structure (PDB code: 1R9O) in this study. All investigated ligands bound deep in the active site pocket in close proximity to the heme. Except for chondroitin, all ligands bonded to residues found in critical secondary structures that formed the boundary of active site cavity including B-C loop, F helix, F-G loop and I helix. A total of 12 amino acids were involved in the binding and all were critical residues located in four out of six substrate recognition sites (SRSs) that have been identified as important substrate binding and catalysis regions in other CYP isoforms. The relatively more potent binding (lower CDOCKER interaction energy) observed for diacerein and rhein compared to glucosamines and C4S are likely due to two main factors: higher number of bonds between ligand molecule and CYP2C9 active site residues (14 versus 0–4), and direct hydrophobic interaction with the heme moiety. The binding residues identified in both diacerein and rhein were the residues that also bonded with sulfaphenazole, the specific and potent CYP2C9 inhibitor. Collectively, the in silico data from this study have provided insights into structural features of CYP2C9 critical for inhibition, and formed basis for further exploration of structural determinants for potency and specificity of some commonly used natural anti-arthritic compounds in CYP2C9 inhibition.
A novel robust memetic algorithm for dynamic community structures detection in complex networks
Authors: Ranjkesh Somayeh , Masoumi Behrooz , Hashemi Seyyed Mohsen 
Published: December 2022

Abstract
Networks in the real world are dynamic and evolving. The most critical process in networks is to determine the structure of the community, based on which we can detect hidden communities in a complex network. The design of strong network structures is of great importance, meaning that a system must maintain its function in the face of attacks and failures and have a strong community structure. In this paper, we proposed the robust memetic algorithm and used the idea to optimize the detection of dynamic communities in complex networks called RDMA_NET (Robust Dynamic Memetic Algorithm). In this method, we work on dynamic data that affects the two main parts of the initial population value and the calculation of the evaluation function of each population, and there is no need to determine the number of communities in advance. We used two sets of real-world networks and the LFR dataset. The results show that our proposed method, RDMA_Net, can find a better solution than modern approaches and provide near-optimal performance in search of network topologies with a strong community structure.
Reliability and construct validity of the new region-specific PBM AP-7D developed in East and Southeast Asia
Authors: Shiroiwa Takeru , Murata Tatsunori , Fukuda Takashi 
Published: December 2022

Abstract
Background: The AP-7D is a preference-based measure newly developed in East and Southeast Asia. This study aimed to show the psychometric properties of the new instrument.
Methods: Surveys were conducted in Japan, Korea, China, Thailand, and Singapore. In each country, 500 respondents were selected by quota sampling and stratified by sex and age. We conducted a face-to-face survey in Japan and a web-based survey in the other countries at two time points. The AP-7D and the EQ-5D-5L were collected from all countries, and the SF-6D was collected only in Japan. The reliability of the instruments was confirmed by comparing responses based on self-report test-retest methods. Regarding construct validity, we compared responses to the AP-7D with those of the EQ-5D-5L and the SF-6D based on predetermined hypotheses.
Results: The percentage of agreement between the two surveys was 73.9% (pain/discomfort), 71.2% (anxiety/depression), 68.6% (energy), 89.6% (mobility), 82.1% (work/school), 84.3% (social relations), and 86.4% (burden to others) in five countries. These values were comparable with those of the EQ-5D-5L. The kappa coefficients of the AP-7D items were similar to those of the EQ-5D-5L. According to the correlation matrix between the AP-7D and EQ-5D-5L, correlation exceeded 0.5 in the following items: 0.69 between “mental health” (AP-7D) and “anxiety/depression” (EQ-5D-5L), 0.54 between both “pain/discomfort,” 0.51 between “energy” (AP-7D) and “anxiety/depression” (EQ-5D-5L), and 0.50 between both “mobility.”
Conclusion: The survey results showed good reliability and construct validity of the AP-7D. Basic psychometric properties of the new instrument were confirmed.
Gut Microbiome Profiles Are Related to Atypical Antipsychotic Associated Overweight in Asian Children with Psychiatric Disorder
Authors: Hao Shao-rui , Zhou Yuan-yue , Zhang Xue , Jiang Hai-yin 
Published: December 2022

Abstract
Atypical antipsychotics (APs) modify the gut microbiome, and weight gain in response to AP could be mediated by the gut microbiome. Thus, the present study aimed to explore the changes in the gut bacterial microbiome in AP-exposed children with obesity. To rule out the confounder of AP indication, the gut bacterial microbiome was compared between healthy controls (Con) and AP-exposed individuals with overweight (APO) or normal weight (APN). Fifty-seven AP-treated outpatients (21 APO and 36 APN) and 25 Con were included in this cross-sectional microbiota study. AP users, regardless of body mass index, exhibited decreased microbial richness and diversity and a distinct metagenomic composition compared to the Con. Although no differences in the microbiota structure were observed between APO and APN groups, the APO group was characterised by a higher abundance of Megamonas and Lachnospira. Additionally, the differences in the microbial functions were observed between APO and APN groups. The gut bacterial microbiota of APO children revealed taxonomic and functional differences compared to Con and APN. Further studies are needed to verify these findings and to explore the temporal and causal relationships between these variables.
Validation of in vitro and in vivo personalized cancer treatment assays for head and neck squamous cell carcinoma
Authors: Wahbi Wafa , Korelin Katja , Sieviläinen Meri , Karihtala Peeter , Wilkman Tommy , Tarkkanen Jussi , Salo Tuula , Al-Samadi Ahmed 
Published: December 2022

Abstract
Background

Head and neck squamous cell carcinoma (HNSCC) is a common cancer with a high heterogeneity and few approved treatments. HNSCC is one of the least explored areas for precision oncology. In this study, we aimed to test the reliability of our three established rapid cancer systemic treatment-testing assays: human tumor-derived matrix (Myogel)-coated well-plates, zebrafish xenografts, and 3D microfluidic chips.

Methods

Chemo-, radio- and targeted-therapy testing in Myogel-coated wells and zebrafish xenografts was conducted nine times using five samples; two primary and three metastatic tumour samples from three HNSCC patients. Peripheral blood mononuclear cells (PBMNCs) were isolated from the patients’ blood. The response of the tumour cells to radio-, chemo-, and targeted therapy was tested using Myogel-coated wells and zebrafish larvae xenografts. The tumour cell’s’ response to immunotherapy was tested using 3D microfluidic chips. The cells’ sensitivity to the treatments was compared with the patients’ clinical response. Primary and metastatic tumour tissue-derived DNA samples from two patients underwent whole exome sequencing to compare the mutational profiles of the samples.

Results

Test results were in line with patients’ responses in 7/9 (77%) zebrafish xenograft assays and 5/9 (55%) Myogel-coated wells assays. Immunotherapy testing was done using one metastatic patient sample which matched the patients’ response. Differences in responses to treatments between primary and metastatic samples of the same patient were detected in 50% of the zebrafish larvae assays.

Conclusions

Our results show the potential of using personalized cancer treatment testing assays – specifically zebrafish xenografts that showed a 100% sensitivity in predicting the true positive response – on HNSCC patient samples.
VALIDITY AND RELIABILITY OF THE TURKISH VERSION OF THE QUESTIONNAIRE FOR PHYSICAL THERAPY STUDENTS’ ATTITUDES TOWARD THEIR PROFESSION AND EDUCATION (Q-PTSAPE)
Authors: TAŞVURAN HORATA Emel , KALKAN Serpil , TAŞKIN Gülşen , TEDLA Jaya Shanker 
Published: December 2022

Purpose: This study aimed to assess the validity and reliability of Turkish version of Questionnaire for Physical Therapy Students’ Attitude toward Their Profession and Education (Q-PTSAPE).
Methods: Included in this study were 488 physical therapy students. The content, construct, and known-groups validities were assessed and the cut-off value was calculated. The construct validity of the questionnaire was evaluated by confirmatory factor analysis (CFA). The Cronbach's alpha and intraclass correlation coefficient (ICC) were calculated for the reliability of the questionnaire.
Results: The content validity index was obtained as 0.97. The construct validity was acceptable (Root Mean Square Error of Approximation=0.061, Comparative Fit Index=0.905, Tucker-Lewis Index=0.892, Goodness of Fit Index=0.887, Normed Fit Index=0.860). Individuals with a Q-PTSAPETurkish score of ≥83.5 have a positive attitude towards their profession and education (p=0.001). Cronbach's alpha reliability coefficient was excellent (0.90). ICC values of the questionnaire and its subdivisions were from medium to high (0.521-0.782).
Conclusion: Q-PTSAPE-Turkish was valid and reliable in determining the attitudes of physiotherapy students towards the profession and vocational education.
Study on the water eutrophication evolution characteristics of Junshan Lake
Authors: Huang Wenfu , Liang Ning , Zhou Long , Lu Jiangang 
Published: December 2022

AbstractIn this paper, the comprehensive nutritional status index (TLI) method was utilized to evaluate the water quality and nutritional status of Junshan Lake from 2018 to 2020. Combining the tools of ‘create fishnet’ and ‘inverse distance weight’ in ArcGIS, the spatial distribution map of the comprehensive trophic index of Junshan Lake was generated. The results show that: (1) The water quality of Junshan Lake was deteriorating year by year. The comprehensive nutritional index (TLI) of Junshan Lake in 2018, 2019 and 2020 were 24.12–31.93, 25.27–35.84, and 26.15–46.87, respectively. The nutritional status of Junshan Lake was dominated by Grade I (Oligotropher) in 2018, and by Grade II (Mesotropher) in 2020; the proportion of water in Grade II (Mesotropher) rose from 24.5% in 2018 to 78.6% in 2020; (2) Aquaculture makes a great contribution to the increase in the lake nutrition level, and the comprehensive nutritional index of the aquaculture area is relatively high; (3) The comprehensive nutritional index value of the water body in the southern part of Junshan Lake is higher than that in the northern part, and the risk of converting to Grade III (light eutrophication) is higher.
Therapeutic Effectiveness of Sinensetin Against Cancer and Other Human
Complications: A Review of Biological Potential and Pharmacological Activities
Authors: Patel Dinesh Kumar , Patel Kanika 
Published: December 2022

Background:
Plant and their active phytoproducts have been used in modern medicine
and playing an important role in the health sectors since a very early age. Human beings need a
considerable amount of these plant-based phytochemicals for their health. The flavonoidal class
phytochemical is an important class of natural products in modern healthcare because of their different
pharmacological activities and health benefits. Flavonoidal class phytochemicals have been
used to treat diabetes and related secondary complications in humans. Flavonoids have antiapoptotic,
anti-hyperlipidemic, anti-inflammatory, and anti-oxidant potential in the health sectors.
Sinensetin, also called 3',4',5,6,7-pentametoksiflavon is a colorless compound with a molecular
weight 372.37g/mol and is found to be present in the Orthosiphon stamineus.

Methods:
In the present investigation, we aim to collect scientific information on sinensetin and
analyze it for its biological potential and therapeutic benefits against various types of disorders and
complications. Medicinal importance and pharmacological activities data have been collected and
analyzed in the present work for sinensetin through literature data analysis of different research
works. Google Science Direct, PubMed, Scopus, and Google Scholar were mainly searched to collect
the scientific information in the present work. The present work analyzed sinensetin biological
potential, pharmacological activities, and analytical aspects.

Results:
Literature data analysis of different scientific research works revealed the biological potential
of phytochemicals in medicine, including flavonoids. Sinensetin has anti-tumor, antiinflammatory,
anti-oxidant, anti-diabetic, and antibacterial activities through their testing in different
in vitro and in vivo models. Sinensetin has physiological functions, including anti-oxidant, antiinflammation,
and anti-cancer potential in medicine. Scientific data analysis signified the biological
importance of sinensetin against tumors, gastric cancer, colorectal cancer, breast cancer, diabetes,
influenza H1N1 infection, obesity, inflammation, colitis, brain disorders, and microbial infections.
Further biological potential of sinensetin on enzymes and angiogenesis has been analyzed in
the present work. Sinensetin was isolated through different analytical and extraction techniques,
including chromatographic techniques.

Conclusion:
Literature data analysis signified sinensetin’s biological potential and pharmacological
activities in medicine.
Phytochemical and Pharmacological Profile of Desmodium styracifolium
(Osbeck) Merr: Updated Review
Authors: Sharma Nidhi , Balkrishna Acharya , Semwal Ankita , Arya Vedpriya 
Published: December 2022

Abstract:
Desmodium styracifolium (Osbeck) Merr. is an important traditional medicinal herb documented
in the indigenous medicinal systems and used in many folk medicines to treat various ailments.
D. styracifolium belongs to the legume family Fabaceae (Leguminosae) and is preferably
abundantly found worldwide. The plant possesses various phytochemical and pharmacological activities.
Plant’s phytochemical accouter confers significant therapeutic properties and has been used
traditionally for treating ailments like pyrexia, stranguria, renal calculi, dysuria, and oliguria. Here,
we provide a comprehensive review of scientific findings of D. styracifolium phytoconstituents and
their related anti-hypertensive, anti-oxidant, anti-urolithic and hepatoprotective activities in nonclinical
in-vitro and in-vivo studies. More than 50 chemical compounds have been reported from D.
styracifolium, including flavonoids, terpenoids, glycosides, phenols, and phytosterols. This paper
presents a comprehensive analysis of the chemical constituents, pharmacological activities, and ethnomedicinal
uses of D. styracifolium, providing scientific evidence for ethnomedicinal claims and
opening perspectives for future research.
Combating Childhood Cancer: Paediatric Patients Living with Neuroblastoma - Regulatory Ramifications and Roadblocks
Authors: Kurz Manfred 
Published: December 2022

Abstract:This paper relates to Neuroblastoma (NBL), a rare, solid cancer affecting children and aims to describe regulatory obligations to adhere to during development, marketing authorisation application (MAA) and post-authorisation stage. The focus is on European Union (EU) paediatric legislation, although essential US Food and Drug Administration (FDA) elements are briefly outlined. Practical regulatory aspects and reporting requirements, players in the therapeutic area as well as clinical management are described. The feasibility and limitations of future harmonised clinical development are pointed out.
Evaluating Clinician Expectations of mHealth Solutions to Increase Rapid-Screening for HIV and Hepatitis in Migrant Populations in France: Qualitative Study
Authors: Brown Carter , Roucoux Guillaume , Dimi Svetlane , Fahmi Saleh , Jeevan Raj-Banou , Chassany Olivier , Chaplin John , Duracinsky Martin 
Published: December 2022

Background
Migrants underuse screening opportunities for HIV, hepatitis B, and hepatitis C despite elevated risk factors for contracting these infections. Language barriers are an often given as reasons for limiting access to services. Translation and communication apps increase communication and overall patient satisfaction in the patient-provider relationship. In the development and adoption of new technology, expectations play an important role.

Objective
This study aimed to explore health care professionals’ opinions and attitudes regarding their screening practices with migrants and their expectations for a new communication tool that could improve migrants’ screening use.

Methods
In this qualitative study, a purposive (diverse) sampling method was used to invite doctors and nurses who conduct rapid screening tests with migrants from 4 centers of the French Office of Immigration and Integration in 3 geographic regions of France. Semistructured interviews were conducted to survey their opinions on the rapid testing of migrants, the use of telephone interpreters, the concept of health literacy, and their expectations of a new communication tool that could overcome language barriers and promote rapid screening in the new migrant population.

Results
In all, 20 interviews were conducted with 11 doctors and 9 nurses with a median age of 58 (range 25-67) years. Participants favored the integration of an innovative communication tool in the context of rapid screening of migrants. However, there were concerns related to the implementation and added value of the tool while migrants were already reluctant to be screened. Expectations were for a tool that would present information in simplified French or a chosen language but also supports a positive attitude toward screening. Health professionals also expressed the wish that the technology could help with the collection of health data.

Conclusions
Feedback from health professionals provides a better understanding of potential formats, characteristics, functions, content, and use of an innovative, digital method to communicate with migrants with limited French proficiency. Findings contribute to the conceptual development of an electronic app and its implementation within the ApiDé study, which aims to validate a digital app to address language barriers to increase the use of screening among migrants with limited French proficiency in France.
Brain-Targeted Drug Delivery System: A Novel Approach
Authors: Chahar Rajeev Kumar , Tiwari Chanchal , Malik Princy , JAISWAL PANKAJ KUMAR 
Published: December 2022

A targeted drug delivery system is based on a technique that continuously administers a predetermined dosage of a therapeutic agent to a sick location of the body. The targeted drug delivery goal is to raise the relative amount of the treatment in the target tissues while lowering it in the non-target tissues. This technique's intrinsic benefit has been reduced drug dose and adverse effects. Drug targeting in the brain is one of the most challenging issues in pharmaceutical research because the blood-brain barrier acts as an impermeable barrier for systemically delivered therapeutics and the brain extracellular matrix contributes to the poor distribution of locally delivered drugs. In the treatment of various Central nervous system (CNS) diseases, general approaches that can improve drug delivery to the brain are of great interest. Drugs are less harmful and more effective when they are administered close to where they would be most effective. Extreme research studies have recently concentrated on the development of fresh strategies for more successfully delivering medications to the brain in response to the shortcomings of the traditional delivery mechanism. This study thoroughly explains the obstacles involved in brain-targeted drug delivery, the process of drug transfer through Blood Brain Barrier, different techniques for brain-targeted drug delivery, and some recent breakthroughs in brain-targeted drug delivery.
Keywords: Blood-brain barrier, Brain-targeted, Cerebrospinal fluid, Nanoparticles, Liposomes, Convection-enhanced drug delivery.
Prevalence of Hepatitis B Core Antibody in Sudanese Random Blood Donors at Khartoum state, 2022
Authors: Ebar Mohamed Hassan Osman , Abdalla Hassan Abass Mohamed , Osman Malaz Almoiz Atiya , Ahmed Albara 
Published: December 2022

Background: Hepatitis B virus is a DNA virus belonging to the Hepadnaviridae family attacking the liver and can cause both acute and chronic disease. The aim of this study was to determine the prevalence of Hepatitis B core antibody in Sudanese random blood donors.
Materials and methods: The study was descriptive cross-sectional study conducted at Ibn Sina Specialized Hospital, Khartoum, Sudan during the period from May 2022 to September 2022. Blood samples were collected from 100 random blood donors. A total of three ml of whole blood were collected from each participant in plain tube. Hepatitis B core antibody test was done by using biochemistry analyzer (Cobas 411).
Results: The results of this study revealed that all blood donors were negative for HBsAg but 51% of them were positive for HBcAb. The mean average of hepatitis B core antibody in blood donors was 0.984 ± 0.950, where the mean average of hepatitis B surface antigen was 0.685± 0.170. The educational background had a positive correlation with the prevalence of hepatitis B core antibody.
Conclusion: This study concluded that the prevalence of hepatitis B core antibody in blood donors was high.
Keywords: Hepatitis B virus infection, hepatitis B core antibody, enzyme linked immunosorbent assay, blood donation, blood transfusion, random blood donors