bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pembuatan Motion Graphic Penanganan Pascapanen Dan Teknologi Hasil Olahan Cabai Di Balai Besar Pascapanen
Authors: Indriasari Sofiyanti 
Published: January 2022

. Balai Besar Pascapanen merupakan unit kerja Badan Penelitian dan Pengembangan Pertanian yang bertugas untuk melaksanakan penelitian dan pengembangan teknologi pascapanen pertanian serta pendayagunaan hasil pertanian pascapanen pertanian seperti penelitian pengolahan hasil olahan cabai menjadi bubuk cabai, minyak cabai, dan pasta cabai. BB Pascapanen telah mengasilkan banyak olahan cabai dari hasil pertanian yang memiliki mutu tinggi dan daya jual yang banyak diminati masyarakat. Informasi yang dipublikasikan saat ini hanya melalui website dan leaflet, sehingga banyak masyarakat belum mengetahui informasi yang ada di BB Pascapanen. Salah satu upayanya adalah dengan pembuatan motion graphic proses teknologi penanganan pascapanen cabai dan motion graphic proses teknologi hasil olahan cabai. Pembuatan motion graphic ini merupakan salah satu upaya dalam penyebaran informasi dan media promosi BB Pascapanen. Metode yang digunakan dalam pembuatan motion graphic melalui tujuh tahapan. Tahapan dibuat sesuai dengan pekerjaan yang dilakukan di tempat praktik kerja lapangan. Tahapan-tahapan tersebut antara lain diskusi, perencanaan, pengumpulan bahan, implementasi, pengujian, revisi dan distribusi. Berdasarkan pada pengujian yang sudah dilakukan dapat disimpulkan bahwa media penyampaian informasi terkait penanganan pascapanen dan teknologi hasil olahan cabai menggunakan motion graphic menjadi pilihan masyarakat di kalangan milenial.
Prácticas docentes de la comprensión de múltiples textos en educación secundaria
Authors: Gómez Cordero Xóchitl , Vega López Norma Alicia 
Published: January 2022

La comprensión de múltiples textos (CMT) o comprensión intertextual, es una de las principales habilidades de pensamiento de orden superior, como la selección, evaluación, integración y síntesis de información proveniente de múltiples textos y formatos. El objetivo del presente artículo fue describir las prácticas docentes (noción CMT, prácticas de enseñanza y evaluación) de la comprensión intertextual por modalidad educativa de los profesores de español en educación secundaria. El método utilizado fue cualitativo, de corte transversal exploratorio y un diseño de casos cruzados por modalidad de secundaria. La técnica de investigación utilizada fue la entrevista semiestructurada, aplicada a 19 profesores. A través de la técnica de análisis de contenido, los resultados muestran que los profesores de secundarias técnicas y privadas, aun cuando tienen una noción de la
CMT, describen procesos de enseñanza concernientes a la comprensión de un sólo texto. Referente a la enseñanza de la evaluación de las fuentes, la mayoría coincide en no realizar tal proceso debido al desconocimiento del tema y falta de tiempo o recursos necesarios en la institución. Estos hallazgos sugieren reflexionar acerca de las necesidades de formación del profesorado.
PERJUANGAN LA ODE NGKADIRI DI KERAJAAN MUNA DALAM MELAWAN BELANDA : 1626-1667
Authors: Wa Sema Wa Sema , Aswati Aswati , Hamuni Hamuni 
Published: January 2022

Tujuan penelitian ini adalah untuk (1) Menjelaskan latar belakang masuknya Belanda di Kerajaan Muna tahun 1613, (2) Menjelaskan alasan perjuangan La Ode Ngkadiri melawan Belanda, (3) Menjelaskan strategi perjuangan La Ode Ngkadiri melawan Belanda, (4) Menjelaskan dampak perjuangan La Ode Ngkadiri dalam melawan Belanda bagi masyarakat Muna. Penelitian ini menggunakan metode sejarah dengan lima tahapan kerja antara lain (1) Pemilihan Topik (2) Heuristik (3) Verifikasi Sumber (4) Interpretasi Sumber (5) Historiografi. Hasil penelitian menunjukkan bahwa (1) Latar belakang kedatangan bangsa Belanda di Kerajaan Muna adalah untuk mengatasi ekonomi di negarannya. Hal tersebut didukung oleh potensi alam wilayah kerajaan Muna yang kaya kayu jati, kapuk, jagung dan komoditas lainnya. Belanda juga berniat ingin menerapkan sistem monopoli perdagangan di Kerajaan Muna. (2) Perlawanan La Ode Ngkadiri terhadap Belanda disebabkan ketidaksukaannya akan sikap Belanda yang selalu memecah belah rakyat Muna demi menanamkan pengaruh kekuasaannya. (3) Strategi La Ode Ngkadiri dalam menentang Belanda yaitu bertindak secara non kooperatif atau tidak mau bekerja sama. La Ode Ngkadiri menghadapi Belanda dengan melakukan penolakan yakni tidak bersedia menerima pemerintahan Belanda untuk memasuki kerajaan Muna. La Ode Ngkdiri membangun strategi dengan mengadakan penjagaan ketat di wilayah kerajaan terutama pelabuhan-pelabuhan yang sering disinggahi kapal-kapal dagang Belanda. (4) Dampak perjuangan La Ode Ngkadiri melawan Belanda bagi masyarakat Muna yakni berbagai aktivitas pemerintahan dan ekonomi masyarakat tidak dapat dilaksanakan dengan baik. Hal ini disebabkan karena perhatian utama masyarakat Muna pada saat itu tertuju pada upaya mendukung perjuangan La Ode Ngkadiri dalam mempertahankan keutuhan wilayah Muna.
A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer
Authors: Ke Zhi-Bin , You Qi , Sun Jiang-Bo , Zhu Jun-Ming , Li Xiao-Dong , Chen Dong-Ning , Su Li , Zheng Qing-Shui , Wei Yong , Xue Xue-Yi , Xu Ning 
Published: January 2022

Objective: To identify ferroptosis-related molecular clusters, and to develop and validate a ferroptosis-based molecular signature for predicting biochemical recurrence-free survival (BCRFS) and tumor immune microenvironment of prostate cancer (PCa).Materials and Methods: The clinical data and transcriptome data of PCa were downloaded from TCGA and GEO database. Ferroptosis-related genes (FRGs) were obtained from FerrDb database. We performed consensus clustering analysis to identify ferroptosis-related molecular subtypes for PCa. Univariate and multivariate Cox regression analysis were used to establish a ferroptosis-based signature for predicting BCRFS. Internal verification, external verification and subgroup survival analysis were then successfully performed.Results: There was a total of 40 differentially expressed FRGs in PCa. We then identified three ferroptosis-related molecular clusters of PCa, which have significantly different immune infiltrating cells, tumor immune microenvironment and PD-L1 expression level. More importantly, a novel ferroptosis-based signature for predicting BCRFS of PCa based on four FRGs (including ASNS, GPT2, NFE2L2, RRM2) was developed. Internal and external verifications were then successfully performed. Patients with high-risk score were associated with significant poor BCRFS compared with those with low-risk score in training cohort, testing cohort and validating cohort, respectively. The area under time-dependent Receiver Operating Characteristic (ROC) curve were 0.755, 0.705 and 0.726 in training cohort, testing cohort and validating cohort, respectively, indicating the great performance of this signature. Independent prognostic analysis indicated that this signature was an independent predictor for BCRFS of PCa. Subgroup analysis revealed that this signature was particularly suitable for younger or stage T III-IV or stage N0 or cluster 1-2 PCa patients. Patients with high-risk score have significantly different tumor immune microenvironment in comparison with those with low-risk score. The results of qRT-PCR successfully verified the mRNA expression levels of ASNS, GPT2, RRM2 and NFE2L2 in DU-145 and RWPE-1 cells while the results of IHC staining exactly verified the relative protein expression levels of ASNS, GPT2, RRM2 and NFE2L2 between PCa and BPH tissues.Conclusions: This study successfully identified three ferroptosis-related molecular clusters. Besides, we developed and validated a novel ferroptosis-based molecular signature, which performed well in predicting BCRFS and tumor immune microenvironment of PCa.
Lyz2-Cre-Mediated Genetic Deletion of Septin7 Reveals a Role of Septins in Macrophage Cytokinesis and Kras-Driven Tumorigenesis
Authors: Menon Manoj B. , Yakovleva Tatiana , Ronkina Natalia , Suwandi Abdulhadi , Odak Ivan , Dhamija Sonam , Sandrock Inga , Hansmann Florian , Baumgärtner Wolfgang , Förster Reinhold , Kotlyarov Alexey , Gaestel Matthias 
Published: January 2022

By crossing septin7-floxed mice with Lyz2-Cre mice carrying the Cre recombinase inserted in the Lysozyme-M (Lyz2) gene locus we aimed the specific deletion of septin7 in myeloid cells, such as monocytes, macrophages and granulocytes. Septin7flox/flox:Lyz2-Cre mice show no alterations in the myeloid compartment. Septin7-deleted macrophages (BMDMs) were isolated and analyzed. The lack of Septin7 expression was confirmed and a constitutive double-nucleation was detected in Septin7-deficient BMDMs indicating a defect in macrophage cytokinesis. However, phagocytic function of macrophages as judged by uptake of labelled E. coli particles and LPS-stimulated macrophage activation as judged by induction of TNF mRNA expression and TNF secretion were not compromised. In addition to myeloid cells, Lyz2-Cre is also active in type II pneumocytes (AT2 cells). We monitored lung adenocarcinoma formation in these mice by crossing them with the conditional knock-in Kras-LSL-G12D allele. Interestingly, we found that control mice without septin7 depletion die after 3–5 weeks, while the Septin7-deficient animals survived 11 weeks or even longer. Control mice sacrificed in the age of 4 weeks display a bronchiolo-alveolar hyperplasia with multiple adenomas, whereas the Septin7-deficient animals of the same age are normal or show only a weak multifocal brochiolo-alveolar hyperplasia. Our findings indicate an essential role of Septin7 in macrophage cytokinesis but not in macrophage function. Furthermore, septin7 seems absolutely essential for oncogenic Kras-driven lung tumorigenesis making it a potential target for anti-tumor interventions.
Interplay Between Thyroid Hormone Status and Pulmonary Hypertension in Graves’ Disease: Relevance of the Assessment in Thyrotoxic and Euthyroid Patients
Authors: Araruna Larisse Vieira Mendes , Oliveira Daniela Camargo de , Pereira Mônica Corso , Moura Neto Arnaldo , Tambascia Marcos Antonio , Zantut-Wittmann Denise Engelbrecht 
Published: January 2022

BackgroundGraves’ disease (GD) is the most common cause of hyperthyroidism and can cause cardiac changes, such as pulmonary hypertension.MethodsThis is a prospective study in which we obtained demographic, clinical, laboratory data and characteristics of the GD, in addition to investigating cardiorespiratory function, focusing on the detection of pulmonary hypertension. Patients were separated into two groups: thyrotoxicosis and euthyroidism. Ninety patients with GD of both sexes, over 18 years of age, were included. The cardiorespiratory assessment included an echocardiographic evaluation, a questionnaire of specific symptoms, spirometry and a six-minute walk test.ResultsThe hyperthyroid group included 42 patients (47.73%) and the euthyroid group 46 patients (52.27%); 78 were women (86.67%). The prevalence of pulmonary hypertension between the hyperthyroidism (48.57%) and the euthyroidism (29.41%) groups was not different. Free thyroxine levels (FT4) (OR 1.266), higher left atrium volume (OR 1.113) and right ventricle diameter were associated with pulmonary hypertension. A direct correlation between FT4 with forced vital capacity (FVC) and forced expiratory volume in the first second (FEV1), as also an inverse correlation between initial oxygen saturation (SpO2) with diagnostic time and drop SpO2 with the ratio between the diastolic velocity E of the mitral flow and the diastolic velocity of the mitral ring (E/e’) were observed in the euthyroid group. An inverse correlation between FT4 levels with walked distance as % of predicted value, and a direct correlation between E/e’ ratio and walked distance as % of predicted value were observed in the hyperthyroid group.ConclusionWe emphasize the importance of a cardiorespiratory reassessment in GD, even after a long-term control of the thyrotoxic state, as we demonstrate that about 30% of these patients remain with PH and are subject to specific treatment.
A Nomogram for Predicting Intraoperative Hemodynamic Instability in Patients With Pheochromocytoma
Authors: Zhang Zhiqiang , Ye Yunlin , Yu Jiajie , Liao Shufen , Pan Weibin , Guo Yan , Jiang Shuangjian , Luo Cheng , Zheng Fufu 
Published: January 2022

PurposeSurgical removal of pheochromocytoma (PCC), including open, laparoscopic, and robot-assisted adrenalectomy, is the cornerstone of therapy, which is associated with high risk of intraoperative and postoperative life-threatening complications due to intraoperative hemodynamic instability (IHD). This study aims to develop and validate a nomogram based on clinical characteristics as well as computed tomography (CT) features for the prediction of IHD in pheochromocytoma surgery.MethodsThe data from 112 patients with pheochromocytoma were collected at a single center between January 1, 2010, and December 31, 2019. Clinical and radiological features were selected with the least absolute shrinkage and selection operator regression analysis to predict IHD then constitute a nomogram. The performance of the nomogram was assessed in terms of discrimination, calibration, and clinical utility.ResultsAge, tumor shape, Mayo Adhesive Probability score, laterality, necrosis, body mass index, and surgical technique were identified as risk predictors of the presence of IHD. The nomogram was then developed using these seven variables. The model showed good discrimination with a C-index of 0.773 (95% CI, 0.683–0.862) and an area under the receiver operating characteristic curve (AUC) of 0.739 (95% CI, 0.642–0.837). The calibration plot suggested good agreement between predicted and actual probabilities. Besides, calibration was tested with the Hosmer–Lemeshow test (P = 0.961). The decision curve showed the clinical effectiveness of the nomogram.ConclusionsOur nomogram based on clinical and CT parameters could facilitate the treatment strategy according to assessment of the risk of IHD in patients with pheochromocytoma.
Bioinformatics Analysis Identifies Potential Ferroptosis Key Genes in the Pathogenesis of Pulmonary Fibrosis
Authors: He Jie , Li Xiaoyan , Yu Mi 
Published: January 2022

Objective: Ferroptosis has an important role in developing pulmonary fibrosis. The present project aimed to identify and validate the potential ferroptosis-related genes in pulmonary fibrosis by bioinformatics analyses and experiments.Methods: First, the pulmonary fibrosis tissue sequencing data were obtained from Gene Expression Omnibus (GEO) and FerrDb databases. Bioinformatics methods were used to analyze the differentially expressed genes (DEGs) between the normal control group and the pulmonary fibrosis group and extract ferroptosis-related DEGs. Hub genes were screened by enrichment analysis, protein-protein interaction (PPI) analysis, and random forest algorithm. Finally, mouse pulmonary fibrosis model was made for performing an exercise intervention and the hub genes’ expression was verified through qRT-PCR.Results: 13 up-regulated genes and 7 down-regulated genes were identified as ferroptosis-related DEGs by comparing 103 lung tissues with idiopathic pulmonary fibrosis (IPF) and 103 normal lung tissues. PPI results indicated the interactions among these ferroptosis-related genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway enrichment and Genome-Ontology (GO) enrichment analyses showed that these ferroptosis-related genes involved in the organic anion transport, response to hypoxia, response to decrease oxygen level, HIF-1 signaling pathway, renal cell carcinoma, and arachidonic acid metabolism signaling pathway. The confirmed genes using PPI analysis and random forest algorithm included CAV1, NOS2, GDF15, HNF4A, and CDKN2A. qRT-PCR of the fibrotic lung tissues from the mouse model showed that the mRNA levels of NOS2 and GDF15 were up-regulated, while CAV1 and CDKN2A were down-regulated. Also, treadmill training led to an increased expression of CAV1 and CDKN2A and a decrease in the expression of NOS2 and GDF15.Conclusion: Using bioinformatics analysis, 20 potential genes were identified to be associated with ferroptosis in pulmonary fibrosis. CAV1, NOS2, GDF15, and CDKN2A were demonstrated to be influencing the development of pulmonary fibrosis by regulating ferroptosis. These findings suggested that, as an aerobic exercise treatment, treadmill training reduced ferroptosis in the pulmonary fibrosis tissues, and thus, reduces inflammation in the lungs. Aerobic exercise training initiate concomitantly with induction of pulmonary fibrosis reduces ferroptosis in lung. These results may develop our knowledge about pulmonary fibrosis and may contribute to its treatment.
Characteristics of the Immune Cell Infiltration Landscape in Gastric Cancer to Assistant Immunotherapy
Authors: Li Chenlu , Pan Jingjing , Jiang Yinyan , Yu Yan , Jin Zhenlin , Chen Xupeng 
Published: January 2022

Background: Gastric cancer (GC) was usually associated with poor prognosis and invalid therapeutical response to immunotherapy due to biological heterogeneity. It is urgent to screen reliable indices especially immunotherapy-associated parameters that can predict the therapeutic responses to immunotherapy of GC patients.Methods: Gene expression profile of 854 GC patients were collected from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets (GSE84433) with their corresponding clinical and somatic mutation data. Based on immune cell infiltration (ICI) levels, molecular clustering classification was performed to identify subtypes and ICI scores in GC patients. After functional enrichment analysis of subtypes, we further explored the correlation between ICI scores and Tumor Mutation Burden (TMB) and the significance in clinical immunotherapy response.Results: Three subtypes were identified based on ICI scores with distinct immunological and prognostic characteristics. The ICI-cluster C, associated with better outcomes, was characterized by significantly higher stromal and immune scores, T lymphocytes infiltration and up-regulation of PD-L1. ICI scores were identified through using principal component analysis (PCA) and the low ICI scores were consistent with the increased TMB and the immune-activating signaling pathways. Contrarily, the high-ICI score cluster was involved in the immunosuppressive pathways, such as TGF-beta, MAPK and WNT signaling pathways, which might be responsible for poor prognosis of GC. External immunotherapy and chemotherapy cohorts validated the patients with lower ICI scores exhibited significant therapeutic responses and clinical benefits.Conclusion: This study elucidated that ICI score could sever as an effective prognostic and predictive indicator for immunotherapy in GC. These findings indicated that the systematic assessment of tumor ICI landscapes and identification of ICI scores have crucial clinical implications and facilitate tailoring optimal immunotherapeutic strategies.
Association Between Perforating Scleral Vessel and Myopic Maculopathy: A Cross-Sectional Study of a Chinese Cohort
Authors: Yu Huimin , Sun Jinfu , Luo Huan , Wang Zhitao , Sun Xufang 
Published: January 2022

Purpose: To investigate the association between perforating scleral vessel (PSV) and different types of myopic maculopathy (MM) in a highly myopic population.Methods: In total, 188 highly myopic eyes (117 participants) were enrolled. Each participant underwent detailed history taking and ocular examinations. Based on fundus photographs and optical coherence tomography, patients were subdivided into the non-MM group and MM group. Based on a new classification system (ATN), MM cases were classified as myopic atrophy maculopathy (MAM), myopic tractional maculopathy (MTM), and myopic neovascular maculopathy (MNM). The number of PSV and the macular choroidal thickness (mChT) were measured.Results: Compared with non-MM group, MM group was characterized by relatively larger age (48.40 vs. 32.34; p &lt; 0.001), longer axial length (AL, 29.72 vs. 27.75, p &lt; 0.001), thinner mChT (52.90 vs. 122.52; p &lt; 0.001), and lower PSV counts (6.73 vs. 9.47, p ≤ 0.001). The non-MM group had higher PSV counts in total area (0–9 mm, 9.47 vs. 6.73, p &lt; 0.001) and perifovea area (3–9 mm, 7.25 vs. 4.71, p &lt; 0.001) compared to the MM group. Univariate and multivariate analyses showed that PSV count had no association with MAM (p = 0.2419) and MTM (p = 0.5678). Total PSV count [odds ratio (OR) 0.78, 95% CI 0.64–0.95, p = 0.0149] and perifovea PSV count (OR 0.80, 95% CI 0.65–0.98, p = 0.0299) were both protective factors for MNM. The stratified analysis revealed that in groups with AL &lt;28 mm, or mChT &lt;50 μm, or mChT ≥100 μm, or eyes with cilioretinal artery, PSV count had no significant association with MNM.Conclusion: Higher PSV counts in perifovea area (3–9 mm centered fovea) and total area (0–9 mm centered fovea) were protective factors for MNM, whereas PSV count had no association with MAM and MTM. These findings may provide novel insights into the mechanisms of pathologic myopia.
Forensic Impact of the Omics Science Involved in the Wound: A Systematic Review
Authors: Ros Aurelia Collados , Bacci Stefano , Luna Aurelio , Legaz Isabel 
Published: January 2022

Background: In forensic autopsies, examining the wounds is one of the most critical aspects to clarify the causal relationship between the cause of death and the wounds observed on the corpse. However, on many occasions, it is difficult to differentiate antemortem injuries from post-mortem injuries, mainly when they occur very close to the moment of death. At present, various studies try to find biomarkers and clarify the molecular mechanisms involved in a wound due to the high variability of conditions in which they occur, thus being one of the most challenging problems in forensic pathology. This review aimed to study the omics data to determine the main lines of investigation emerging in the diagnosis of vital injuries, time of appearance, estimation of the age and vitality of the wound, and its possible contributions to the forensic field.Methods: A systematic review of the human wound concerning forensic science was carried out by following PRISMA guidelines.Results: This study sheds light on the role of omics research during the process of wounding, identifying different cytokines and other inflammatory mediators, as well as cells involved in the specific stage of the wound healing process, show great use in estimating the age of a wound. On the other hand, the expression levels of skin enzymes, proteins, metal ions, and other biomarkers play an essential role in differentiating vital and post-mortem wounds. More recent studies have begun to analyze and quantify mRNA from different genes that encode proteins that participate in the inflammation phase of a wound and miRNAs related to various cellular processes.Conclusions: This study sheds light on the role of research in the molecular characterization of vital wounds, heralding a promising future for molecular characterization of wounds in the field of forensic pathology, opening up an important new area of research.Systematic Review Registration: URL: https://www.crd.york.ac.uk/prospero/#myprospero, Identifier: CRD42021286623.