bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monthly potential evapotranspiration estimated using the Thornthwaite method with gridded climate datasets for Rio de Janeiro state, Brazil
Authors: Santos Christiane Nascimento , Santos Anderson Amorim Rocha , Abreu Marcel Carvalho , Martins Fabrina Bolzan , Lyra Guilherme Bastos , Souza José Leonaldo , Lyra Gustavo Bastos 
Published: August 2022

Abstract
Monthly potential evapotranspiration (PET) using the Thornthwaite (ThW) method fed with gridded climate datasets was evaluated relative to PET by ThW and to reference evapotranspiration (ETo) estimated using the FAO56 Penman-Monteith method (PM-FAO56) with observed data, for Rio de Janeiro state, and neighboring states in southeast Brazil. The PM-FAO56 method used monthly climate series (1961–2010) on sunshine hours, air temperature, relative humidity, and wind speed, from 21 weather stations of the National Institute of Meteorology (INMET), in Rio de Janeiro (RJ), São Paulo (SP), Minas Gerais (MG), and Espírito Santo (ES) to estimate ETo. The estimated PET using the ThW method was based on observed air temperature from INMET stations and also using gridded air temperature datasets (1961–2010) from the Global Historical Climatology Network (GHCN) and the University of Delaware (UDel). The ETP estimates had an agreement (Willmott index) between 0.54 ≤ dw ≤ 0.96 – GHCN and 0.62 ≤ dw ≤ 0.96 - UDel; a precision between 0.44 ≤ r² ≤ 0.88 – GHCN and 0.53 ≤ r² ≤ 0.95 - UDel; and RMSE inferior to 1.39 mm d− 1 – GHCN and 1.13 mm d− 1 - UDel. The PET estimated with the UDel gridded series was better overall performance than using the GHCN product. Differences in altitude, latitude and longitude were the main geographic variables determining the precision and agreement of the PET estimates using GHCN and Udel. The gridded datasets are an alternative for locations without climatic series data or with low-quality non-continuous data series.
Clinical outcomes of implantation of posterior chamber phakic intraocular lens for pathologic and non-pathologic myopia
Authors: Caixia Lin , Yawen Bai , Yuxin Fang , Xiaoxia Li , Yuhan Wang , Ke Yang , Liya Qiao 
Published: August 2022

Abstract
Background
To compare the clinical outcomes of posterior chamber phakic intraocular lens (pIOL) implantation for non-pathological myopia and pathological myopia.
Methods
This retrospective case series study which were conducted in Beijing Tongren Eye Center between July 2017 and Oct 2021 comprised 192 eyes of 100 consecutive patients undergoing pIOL implantation. Eyes were divided into two groups based on having pathological myopia or not. Predictability, efficacy, safety, and adverse events were compared at 6 months after pIOL implantation.
Results
Our study included 86 non-pathological myopes (171 eyes, group1) and 14 pathological myopes (21eyes, group2) to analysis. The average ages were 25.5 and 33.0, respectively, and the spherical equivalent (SE) were − 9.31D and − 17.50D pre-operation. Six months after pIOL implantation, the SE were 0.00 and − 0.50, respectively, and the refraction changes were statistically significant (P ≤ 0.05). Six months after surgery, 76.92% and 80.41% were within ± 0.50 D of the target and 92.31% and 95.88% were within ± 1.00 D. All eyes had unchanged BCVA or gained 1 or more lines in both groups and mean BCVA both improved a line 6m after operation. The efficacy index in the two groups were 0.95 and 0.88 and the safety index were 1.20, 1.33, respectively which was significantly different (P ≤ 0.05). Over the 6-month follow-up, no cataract, pigment dispersion glaucoma, pupillary block, or other vision-threatening complications happened, either.
Conclusions
The pIOL performed well for the correction of both non-pathological and pathological myopia throughout the 6-month observation period. The clinical outcomes of pIOL implantation for non-pathological myopia are essentially equivalent to those for pathological myopia.
Temporal Localized Turing Patterns in Vertical External-Cavity Surface-Emitting Laser
Authors: Bartolo Adrian , Vigne Nathan , Marconi Mathias , Beaudoin Grégoire , Pantzas Konstantinos , Sagnes Isabelle , Huyet Guillaume , Maucher Fabian , Gurevich Svetlana , Javaloyes Julien , Garnache Arnaud , Giudici Massimo 
Published: August 2022

Abstract
We show that large aspect-ratio Vertical External-Cavity Surface-Emitting Lasers (VECSELs) with a saturable absorber can be operated in the regime of spatio-temporal mode locking. The emitted pulses exhibit a spatial profile resulting from the phase locking between an axial plane-wave with a set of tilted waves having a hexagonal arrangement in the Fourier space. We show that these pulsating patterns are temporally localized, i.e. they can be individually addressed by modulating the optical pump.The theoretical analysis shows that the emergence of these pulsating patterns is a signature of a Turing instability whose critical wave vector depends on spherical aberrations of the optical elements. Our result reveals that large aspect-ratio VECSELs offer unique opportunities for studying fully developed spatio-temporal dynamics.
OsPEX1, an extensin-like protein, negatively regulates root growth in a gibberellin-mediated manner in rice
Authors: Li Jieni , Zhang Yuexiong , Li Zhenyong , Dai Hang , Luan Xin , Zhong Tianxiu , Chen Shu , Xie Xin-Ming , Qin Gang , Zhang Xiang-Qian , Peng Haifeng 
Published: August 2022

Abstract
Leucine-rich repeat extensins (LRXs) are required for plant growth and development through affecting cell growth and cell wall formation. LRX gene family can be classified into two categories: predominantly vegetative-expressed LRX and reproductive-expressed PEX. In contrast to the tissue specificity of Arabidopsis PEX genes in reproductive organs, rice OsPEX1 are also highly expressed in roots in addition to reproductive tissue. However, whether and how OsPEX1 affects root growth is unclear. Here, we found that overexpression of OsPEX1 in the pex1 mutant retarded root growth by reducing cell elongation resulting from an increase of lignin deposition, whereas knockdown of OsPEX1 had an opposite effect on root growth, indicating that OsPEX1 negatively regulated root growth in rice seedling. Upon further investigation, we uncovered the existence of a feedback loop between OsPEX1 transcription and GA biosynthesis for proper root growth. This was supported by the facts that exogenous GA3 application downregulated the transcription levels of OsPEX1 and lignin-related genes, rescuing the development defects of the root in the pex1 mutant, whereas OsPEX1 negatively regulated GA level by altering the expression of GA biosynthesis genes. Moreover, OsPEX1 and GA showed antagonistic action on the lignin biosynthesis in root. OsPEX1 overexpression upregulated the transcription levels of lignin-related genes, whereas exogenous GA application downregulated their expression, accompanied by altered root growth phenotypes. Taken together, our studies reveal a possible molecular pathway of OsPEX1-mediated root growth through coordinate modulation of lignin deposition via a negative feedback regulation between OsPEX1 transcription and GA biosynthesis.
Chemical composition of new varietal honeys and they antimicrobial activities
Authors: Kunat Magdalena , Rysiak Anna , Wiater Adrian , Grąz Marcin , Andrejko Mariola , Budzuński Michał , Bryś Maciej S. , Sudziński Marcin , Tomczyk Michał , Gancarz Marek , Rusinek Robert , Ptaszyńska Aneta A. 
Published: August 2022

Abstract
Nowadays, there is an urgent need to look for antimicrobial substances due to a widespread occurrence of multidrug resistant bacterial and fungal strains. Honey with its antimicrobial properties is a very promising substance with many valuable properties. In this study we examined for the first time antimicrobial properties of novel varietal honeys. The experiments were carried out with 11 honey samples, i.e.: Plum, Rapeseed, Lime, Phacelia, Honeydew, Sunflower, Willow, and Multifloral-P (Prunus spinosa L.), Multifloral-AP (Acer negundo L., Prunus spinosa L.), Multifloral-Sa (Salix sp.), Multifloral-Br (Brassica napus L.). Their antimicrobial activity was tested against bacteria (such as Escherichia coli, Bacillus circulans, Staphylococcus aureus, Pseudomonas aeruginosa), yeasts (such as Saccharomyces cerevisiae and Candida albicans) and mold fungi (such as Aspergillus niger). In tested honeys, phenolic acids were one of the most important group of compounds with antimicrobial activity. In our study, the highest amount of phenolic acids was found in honeydew honey (808.05 µg GAE/g) with the highest antifungal activity aiming A. niger. Among the tested phenolic acids, caffeic acid was in the greatest amount, which was found in the highest amounts in honeys as: phacelia − 356.72 µg/g, multifloral (MSa) and multifloral (MBr) − 318.9 µg/g. The highest bactericidal activity against S. aures was found in multifloral honeys MSa and MBr. Additionally, the highest amounts of syringic acid and cinnamic acid were identified in rapeseed honey. Multifloral honey (MAP) showed the highest bactericidal activity against E. coli, and multifloral honey (MSa) against S. aureus. Additionally, multifloral honey (MBr) was effective against both bacteria: E. coli and S. aureus. Novel varietal honeys tested in our study show various levels of antibacterial and antifungal activity and alhough they had some limitations, they can still enhance drug treatment against multidrug resistant bacterial and fungal strains.
Zurbarán attribution hypothesis supported by pigment analysis and hyperspectral imaging of four paintings by his workshop
Authors: Moreno-Soto Javier , Križnar Anabelle , Ager Francisco José , Morón María Auxiliadora Gómez , Osuna Antonio Gamero , Soto Agustín Martín , Respaldiza Miguel Ángel 
Published: August 2022

Abstract
Francisco de Zurbarán was one of the greatest painters of the Spanish Golden Age, whose artworks are scattered all over the world. Unfortunately, there are hardly any exhaustive studies on the pigments that he used. In this work, four canvas paintings attributed to the Zurbarán Workshop were studied. Each of them presents the figure of a different saint in a peculiar scene isolation: Saint Bruno, Saint Francis of Assisi, Saint Dominic of Guzman, and Saint Peter Martyr. Nevertheless, the painting of Saint Peter Martyr shows a superior quality in both technique and colours, so it is not clear whether this canvas was also made by the Workshop or actually by Zurbarán himself, as some art historians claim.Coinciding with conservation and restoration works, the paintings were analysed initially with non-invasive techniques such as ultraviolet photography (UV), infrared reflectography (IRR) and X-ray fluorescence (XRF) for the characterization of the pigments. Those studies were complemented by stratigraphic analysis of some extracted samples with optical microscopy (OM) and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX). Among other results, we found significant differences between the pigment palette used in Saint Peter Martyr and the other paintings, supporting the hypothesis that this painting was executed by Zurbarán himself instead of his workshop. These results could also help to distinguish other authentic paintings by the master’s hand from very similar ones by his workshop or by other artists.
Application of a time-delay SIR model with vaccination in COVID-19 prediction and its optimal control strategy
Authors: Dong Suyalatu , Xu Lin-Lin , A Yana , Lan Zhong-Zhou , Xiao Ding , Gao Bo 
Published: August 2022

Abstract
In the classical infectious disease compartment model, the parameters are fixed. In reality, the probability of virus transmission in the process of disease transmission depends on the concentration of virus in the environment, and the concentration depends on the proportion of patients in the environment. Therefore, the probability of virus transmission changes with time. Then how to fit the parameters and get the trend of the parameters changing with time is the key to predict the disease course with the model. In this paper, based on the US COVID-19 epidemic statistics during calibration period, the parameters such as incidence rate and recovery rate are fitted by using the linear regression algorithm of machine science, and the laws of these parameters changing with time are obtained. Then a SIR model with time delay and vaccination is proposed, and the optimal control strategy of epidemic situation is analyzed by using the optimal control theory and Pontryagin maximum principle, which proves the effectiveness of the control strategy in restraining the spread of COVID-19. The numerical simulation results show that the time-varying law of the number of active cases obtained by our model basically conforms to the real changing law of the US COVID-19 epidemic statistics during calibration period. In addition, we have predicted the changes of the number of active cases in the COVID-19 epidemic in the United States over time in the future beyond the calibration cycle, and the predicted results are more in line with the actual epidemic data.
Normalization of the Tumor Marker and a Clear Resection Margin Affect Progression-Free Survival of Patients with Unresectable Pancreatic Cancer who have Undergone Conversion Surgery
Authors: Li Xiang , Liu Xinyuan , Lu Na , Chen Yiwen , Zhang Xiaochen , Guo Chengxiang , Xiao Wenbo , Xue Xing , Sun Ke , Wang Meng , Gao Shunliang , Shen Yan , Zhang Min , Wu Jian , Que Risheng , Yu Jun , Bai Xueli , Liang Tingbo 
Published: August 2022

Abstract
Background
With the advent of intensive combination systemic regimens, an increasing number of patients with UPC regain the opportunity of operation. To investigated the clinical benefits and prognostic factors of conversion surgery (CS) in patients with unresectable pancreatic cancer (UPC).
Methods
We retrospectively enrolled patients with UPC who had received CS following first-line systemic treatment in our center between 2014 to 2022. Treatment response, safety of the surgical procedure and clinicopathological data were collected. We analyzed the prognostic factors for postoperative survival among UPC patients who had CS.
Results
Sixty-seven patients with UPC were enrolled (53 with locally advanced pancreatic cancer (LAPC) and 14 with metastatic pancreatic cancer (MPC)). The duration of preoperative systemic treatment was 4.17 months for LAPC patients and 6.52 months for MPC patients. All patients experienced a partial response (PR) or stable disease (SD) preoperatively according to imaging. Tumor resection was unsuccessful in four patients and, finally, R0 resection was obtained in 81% of cases. Downstaging was determined pathologically in 87% of cases; four patients achieved a complete pathological response. Median postoperative-progression-free survival (PO-PFS) was 9.77 months and postoperative overall survival (PO-OS) was 31.2 months. Multivariate logistic regression analyses revealed that the resection margin and postoperative changes in levels of tumor markers were significant prognostic factors for PO-PFS. No factors were associated significantly with PO-OS according to multivariate analyses.
Conclusions
CS is a promising strategy for improving the prognosis of UPC patients. The resection margin and postoperative change in levels of tumor markers are the most important prognostic factors for prolonged PFS.
Knockdown of circHomer1 in the orbitofrontal cortex results in differential encoding of salient stimuli
Authors: Zimmerman Amber , Weick Jason , Papageorgiou Grigorios , Mellios Nikolaos , Brigman Jonathan 
Published: August 2022

Abstract
BackgroundCircHomer1 is an activity-dependent circular RNA (circRNA) isoform produced from back-splicing of the Homer1 transcript. Homer1 isoforms are well-known regulators of homeostatic synaptic plasticity through post-synaptic density scaffold regulation. Homer1 polymorphisms have been associated with psychiatric diseases including schizophrenia (SCZ) and bipolar disorder (BD). Postmortem tissue from patients with SCZ and BD showed reduced circHomer1 levels within the orbitofrontal cortex (OFC), a region associated with behavioral flexibility. While dysregulation of circHomer1 expression has recently been identified across multiple psychiatric and neurodegenerative disorders and is associated with impaired behavioral flexibility in mice, it is unknown whether circHomer1 can induce electrophysiological signatures relevant to cognitive dysfunction in these disorders.MethodsCircHomer1 was knocked down in bilateral orbitofrontal cortex of C57BL/6J male mice and in vivo microarray recordings were captured throughout a touchscreen reversal learning task to identify electrophysiological changes associated with reduction of circHomer1. Following task completion, qRT-PCR was used to quantify transcriptional changes following circHomer1 knockdown.ResultsKnockdown of circHomer1 within the OFC induced robust changes in multiunit firing rate and local field potential coordination and power to salient stimuli during reversal learning. Further, these electrophysiological changes were associated with transcriptional downregulation of glutamatergic signaling effectors and behavioral alterations leading to impaired cognitive flexibility.ConclusionsCircHomer1 is a stable biomolecule, whose knockdown in rodent OFC produces lasting electrophysiological and transcriptional changes important for efficient reversal learning. This is, to our knowledge, the first demonstration of a psychiatric-associated circRNA contributing to electrophysiological, transcriptional, and behavioral alterations relevant to psychiatric phenotypes.
Ecological Status of Temporary Wetlands in Central Tamilnadu District, India.
Authors: Krishnaraj Varunprasath , mathesh Subha 
Published: August 2022

Abstract
Temporary wetlands (TW) are distinguished by a distinct collection of uncommon and specialised flora and fauna species, modest size, periodical drying, and plentiful water during the flood season. The Indian subcontinent is home to a diverse range of freshwater, saline, and marine wetlands. The bulk of the inward wetlands are manmade or transitory, and they have traditionally been used by regional human groups. Most limnological research in India have focused on large, permanent bodies of water, pond ecosystems, and associated temporary wetlands.Furthermore, numerous Temporary Wetlands (TW) in Tamil Nadu State (TNS) offer significant potential for ecosystem service applications but are not yet well documented. Due to a lack of study, the existing survey explores the Ecological status (ES), distribution of Temporary Wetlands (TW), and individual wetland human disturbance score (HDS) in the Central Tamil Nadu district (CTND) in India. Seventy-five selected wetlands were surveyed using a variety of approaches in three districts: Karur, Namakkal, and Trichy. During the summer, all wetlands were hydrologically separated and parched.The TW size ranged from 1 to 10 acres, with 80 percent located in rural regions, 12 percent in semi-urban areas, and 8 percent in metropolitan areas. Wetland degradation was highest in Trichy district wetlands (TDW) at 76 percent Mid Impact (MI), followed by Namakkal district wetlands (NDW) at 36 percent MI, and Karur district wetlands (KDW) at 20 percent MI. Wetland degradation is often caused by landscape disturbance, buffer zone modification, hydrological alteration, pollution, and ecosystem alteration. The current study provides baseline facts on the biological state and distribution of Temporary Wetlands in CTND, which could help in the creation of provincial wetland management strategy. This method will fit other sections of TW and detect ecological status and human effect on wetlands, which will improve TW conservation and management decisions.
ALKBH5 alleviates hypoxia postconditioning injury in aged cardiomyocytes by regulating STAT3
Authors: Zhang Xuan , Li Feixiang , Zhang Xiaobei , Yin Yiqing 
Published: August 2022

Abstract
Ischemic postconditioning (I/Post) reduces ischemia/reperfusion (I/R) injury by activating endogenous cardioprotection mechanisms, such as the JAK/STAT3 and PI3K/Akt pathways, which offer a traditional approach to myocardial protection. According to the previous study, cardioprotection by I/Post may lost in aged mice, and in our previous research, hypoxic postconditioning (H/Post) lacked a protective effect in senescent cardiomyocytes, which was associated with low expression of long noncoding RNA (lncRNA) H19. The N6-methyladenosine (m6A) modification is a dynamic and reversible process that has been confirmed plays a role in cardiovascular diseases. However, the mechanisms of m6A modification in myocardial I/Post remains to be explored. Neonatal cardiomyocytes were isolated from 2-day-old Sprague Dawley rats and senescence was induced by D-galactose, followed by stimulation of hypoxia-reoxygenation (H/R) and H/Post. Hypoxic injury was evaluated by cell viability and the Bcl-2/Bax protein ratio. Total m6A levels were measured using a colorimetric m6A RNA Methylation Quantification Kit and the m6A modified and differentially expressed mRNA was determined by methylated RNA immunoprecipitation (MeRIP). We found that H/Post increased m6A methylation and decreased RNA mA demethylase alkB homolog 5 (ALKBH5) expression in aged cardiomyocytes. Furthermore, ALKBH5 knockdown exacerbated injury following H/Post in senescent cardiomyocytes. Additionally, ALKBH5 regulated STAT3 expression by mediating its m6A modification and lncRNA H19/miR-124-3p. ALKBH5 also alleviated the H/Post injury induced by the low expression of STAT3 in aged cardiomyocytes.
Simulation study on disaster evolution of ventilation system during coal and gas outburst
Authors: Wang Yadi , Li Zongxiang , Rong Hai , Li Nannan 
Published: August 2022

Abstract
In order to solve the serious secondary disasters of the gas overrun or gas explosion, which is caused by coal and gas outburst in mine. In this paper, the equations of fluid transients, convection diffusion and source-containing ventilation network were established respectively to research the dynamic effect and gas migration process of gas outburst in ventilation system of coal mine. In order to research the unsteady movement characteristics of mine airflow under the joint action of gas outburst source flow power and fan ventilation, we completed the experiment of ventilation pipe network system for gas outburst. The fitting equation of gas dispersion parameters under different wind speeds was obtained, which was used as the simulation calculation parameter. In this paper, NC2.0, one simulation software of coal and gas outburst, was developed, and the 10.27 coal and gas outburst accident in Jiaozuo Jiulishan coal mine was reappeared by it. Throughout the simulation process, unsteady air flow and gas distribution in Jiulishan coal mine after coal and gas outburst were simulated respectively by the given fluid changing curve. The research results indicate that the outburst pressure after coal and gas outburst, which is the main factor leading to the changing airflow, and radiates in the form of wave. The high-intensity air flow from the gas outburst source changes the air flow movement of the mine system, causes over air flow phenomenon in return air roadway, suppresses the air volume in air inlet roadway at the same time. Even countercurrent occurs in severe cases. The research results provide the theoretical basis for disaster prevention and aid, reducing loss and preventing secondary disasters in outburst mine.