bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploratory behaviour of territorial passerines toward a novel object in different anthropogenic habitats
Authors: Firoozkoohi Sahar , Paterson Adrian , Sullivan Jon 
Published: March 2022

Abstract
Invasion of a new environment brings novel challenges that favour species with adaptive traits. Western European blackbird (Turdus merula merula) and song thrush (Turdus philomelos) have been introduced into new habitats worldwide. We examined neophobia and neophilia responses toward a novel object in different anthropogenic New Zealand environments, including urban and rural habitats. Urban inhabitants approached a novel object closer and at a higher rate compared to rural dwelling individuals. Blackbirds showed a higher level of individual approach compared to song thrushes, but approach distance among those song thrushes that approached was closer than blackbirds. Birds living in urban habitats may reduce neophobic responses where there is increasing anthropogenic stressors through increased interactions, competition over space, food and mate would be higher than rural habitats.
Feasibility &amp; Preliminary Efficacy of Structured Programming and a Parent Intervention to Mitigate Accelerated Summer BMI Gain: A pilot study
Authors: Weaver R Glenn , Armstrong Bridget , Adams Elizabeth , Beets Michael , White James , Flory Kate , Wilson Dawn , Mclain Alex , Tennie Brianna 
Published: March 2022

Abstract
BackgroundThis study assessed initial feasibility and preliminary efficacy of providing children a free summer day camp and a parent intervention to improve self-regulation and mitigate accelerated summer BMI gain.MethodsThis pilot 2x2 factorial randomized control trial used a mixed methods design to evaluate providing children a free summer day camp (SCV), a parent intervention (PI), and the combination of these two strategies (SCV + PI) to mitigate accelerated summer body mass index (BMI) gain. Feasibility (i.e., recruitment capability, retention, compliance, treatment fidelity, acceptability) was examined using means, standard deviations, and percentages for relevant variables. Changes in BMI were estimated using intent-to-treat and post-hoc dose response analyses via multilevel mixed effects regressions.ResultsA total of 89 families participated, with 24 participants randomized to the PI group, 21 randomized to the SCV group, 23 randomized to the SCV + PI group, and 21 randomized to the control. Parents and children found the summer program acceptable but attendance at the summer program and engagement in the PI were low due to COVID-19 and lack of transportation. Intent-to-treat analyses showed no statistically significant difference between groups in summer BMI gain. Post-hoc dose response analyses showed that for each day (0 to 29) of summer programming children attended they gained − 0.009 (95CI= -0.018, -0.001) less in BMI z-score.ConclusionsEngagement in both the SCV and PI was not ideal and was likely due to COVID-19 and lack of transportation. Providing children with structured summer programming to mitigate accelerated summer BMI gain may be an effective strategy. Thus, a larger trial may be warranted, but more work is needed to ensure children attend the programming.Trial registration: The trial reported herein was prospectively registered at clinicaltrials.gov. Trial #:NCT04608188.
Flood Susceptibility Assessment Using Geospatial Multi-Criteria Framework: A Study in Lower Mahananda Basin, India
Authors: Paul Pranay , Sarkar Rumki 
Published: March 2022

Abstract
Flood is the common natural hazard in the plains of Lower Ganga that causes considerable damage to the society and its infrastructure every year. During the monsoon, Mahananda river carries voluminous discharge and often it overtops the bank and spills out to the surrounding plains, especially in the northern parts of Bihar and West Bengal. Therefore, an attempt has been made to identify and assess the flood risk prone area in the comprised zone of Lower Mahananda basin, India. Seven geospatial parameters have been incorporated to the weighted overlay analysis in GIS environment using multi-criteria based analytical hierarchy process (AHP). The prepared final map is classified into five spatial zones considering their susceptibility to the flood. In this regard, cross-sectional profile of the river at six different sites was made for assessing the water level during the flood. The very high flood susceptible zone comprises about 64.46 km2 area (0.80%) and remains waterlogged for most part of the year. The high flood susceptible zone is consists of 1441.38 km2 of area (17.99%) and widely spread over the populated zone of Malda district when water level in the channels can rise up to 3m from its normal level. The bulk of the area (56.02%) falls in the moderate category and the chances of inundation here only during the major floods. The reliability of the model has been assessed using the AUC, where the accuracy is more than 80%. The overall framework is thus useful for policy implementation and mitigation purposes.
Infection control for COVID-19 in hospital examination room
Authors: Takada Mamoru , Fukushima Taichi , Ozawa Sho , Matsubara Syuma , Suzuki Takeshi , Fukumoto Ichiro , Hanazawa Toyoyuki , Otsuka Masayuki , Tanaka Gaku 
Published: March 2022

Abstract
Healthcare providers are vulnerable to infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) because of their close proximity to patients with coronavirus disease 2019. SARS- CoV-2 is mainly transmitted via direct and indirect contact with respiratory droplets, and its airborne transmission has also been identified. However, evidence for environmental factors is scarce, and evidence-based measures to minimize the risk of infection in clinical settings are insufficient. Using computational fluid dynamics, we simulated exhalation of large and small aerosol particles by patients in an otolaryngology examination room, where medical procedures require the removal of a face mask. The effects of coughing were analyzed, as well as those of humidity as a controllable environmental factor and of a suction device as an effective control method. Our results show that a suction device can minimize aerosol exposure of healthcare workers by efficiently removing both large (11.6–98.2%) and small (39.3–99.9%) aerosol particles. However, for coughing patients, the removal efficiency varies inversely with the particle size, and the humidity notably affects the aerosol behavior, indicating the need for countermeasures against smaller aerosols. Overall, these results highlight the potential and limitation of using a suction device to protect against SARS-CoV-2 and future respiratory infections.
Clinical and radiographic assessment of peripheral joints in controlled acromegaly
Authors: Pelsma Iris C.M. , Kroon Herman M. , Trigt Victoria R. , Pereira Alberto M. , Kloppenburg Margreet , Biermasz Nienke R. , Claessen Kim M.J.A. 
Published: March 2022

Abstract
PurposeAcromegalic arthropathy is a well-known phenomenon, occurring in most patients regardless of disease status. To date, solely hips, knees, hands, and spinal joints have been radiographically assessed. Therefore, this study aimed to assess the prevalence of joint symptoms and radiographic osteoarthritis (OA) of new, and established peripheral joint sites in well-controlled acromegaly. MethodsFifty-one acromegaly patients (56% female, mean age 64±12years) in long-term remission for 18.3 years (median, IQR 7.2 – 25.4) were included. Nineteen patients currently received pharmacological treatment. Self-reported joint complaints were assessed using standardized interviews. Self-reported disability of the upper and lower limbs, and health-related quality of life (HR-QoL) were evaluated using validated questionnaires. Radiographic OA (defined as KL≥2) was scored using (modified) Kellgren&Lawrence (KL) methods. ResultsRadiographic signs of OA were present in 46 patients (90.2%), which occurred in a generalized manner in virtually all patients (N=44; 95.7%). Radiographic MTP1 OA was as prevalent as radiographic knee OA (N=26, 51.0%), and radiographic glenohumeral OA was similarly prevalent as hip OA (N=21 (41.2%) vs N=24 (47.1%)). Risk factors for radiographic glenohumeral OA were higher pre-treatment IGF-1 levels (OR 1.06 (1.01 – 1.12), P=0.021), and current pharmacological treatment (OR 5.01 (1.03 – 24.54), P=0.047), whereas no risk factors for MTP1 joint OA could be identified.ConclusionSimilar to previously-assessed peripheral joints, clinical and radiographic arthropathy of the shoulder and feet were prevalent in controlled acromegaly. Further studies on adequate management strategies of acromegalic arthropathy are needed.
Viral metagenomics reveals a novel human papillomavirus in anogenital wart tissues
Authors: Hu Hui-Min , Ling Yu , Wang Xuan , Wang Hao , Zhu Nian-Nan , Li Yu-Mei , Xu Hui , Zhang Wen 
Published: March 2022

Abstract
Background: Viral metagenomics is widely employed to identify novel virus in biological samples. Recently, although numerous novel HPV strains have been identified in clinical samples including anogenital warts (AGWs), many unknown HPV sequences remain to be discovered. Methods: 110 tissue samples of wart were collected from AGW patients, including 34 female and 76 male. Viral metagenomics was used to detect the viral nucleic acid and obtain the virus genomes from samples. Phylogenetic analysis was performed to infer the evolutionary relationships of novel HPV found here among predicted ORFs on the basis of the closest related HPVs and the most representative strain of α, β and γ genus. Epidemiological survey of novel HPV was performed by using the nested PCR method. Results: A novel HPV designated as HPV-JDFY01 was discovered from a library. The complete genomic sequence of HPV-JDFY01 was a 7,186 bp full-length double-stranded DNA with 7 open reading frames (ORFs). Phylogenetic analysis indicated that HPV-JDFY01 shared the highest sequence identity of 74.2% with HPV-mSK_244 (MH777383) and was clustered into the genus Gammapapillomavirus. Epidemiological investigation showed that one positive sample was screened out by specific screening PCR primers among 413 tissue samples from AGWs. Conclusions: This is the first time to report a novel Gammapapillomavirus identified by viral metagenomics from AGW in China. However, functional, biophysical and carcinogenic properties of HPV-JDFY01 are needed in the future research.
Validation and Prognostic Value of EZ-ALBI Score in Patients with Intermediate-Stage Hepatocellular Carcinoma Treated with Trans-Arterial Chemoembolization
Authors: Ananchuensook Prooksa , Sriphoosanap Supachaya , Suksawatamnauy Sirinporn , Siripon Nipaporn , Pincharoen Nutcha , Geratikornsupuk Nopavut , Thanapirom Kessarin , Komolmit Piyawat 
Published: March 2022

Abstract
BackgroundHeterogeneity of liver function and tumor burden in intermediate-stage hepatocellular carcinoma (HCC) results in different outcomes after transarterial chemoembolization (TACE). Easy albumin-bilirubin (EZ-ALBI), a simplified albumin-bilirubin (ALBI) score, has recently been proposed as a new prognostic score for HCC. This study aimed to validate the EZ-ALBI score and evaluate the impact of dynamic changes in patients with intermediate-stage HCC undergoing TACE.MethodsAll patients with HCC treated with TACE at King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between January 2015 and December 2019 were prospectively enrolled. Intermediate-stage HCC was defined as Barcelona Clinic Liver Cancer (BCLC) stage B or unresectable single HCC with size >5 cm in BCLC stage A. EZ-ALBI and ALBI scores were calculated and stratified into three different grades. Overall survival (OS) and prognostic factors were assessed using the Kaplan-Meier curve and Cox proportional hazard model.ResultsAmong 672 patients with HCC treated with TACE, 166 patients with intermediate-stage HCC who met the eligibility criteria were enrolled. The median OS of all patients in the cohort was 21 months. A good correlation between the EZ-ALBI and ALBI scores was observed (correlation coefficient 1.000, p<0.001). The baseline EZ-ALBI grades 1, 2, and 3 were 24.5%, 70%, and 5.5%, respectively. EZ-ALBI grade can stratify patients with significantly different prognoses (p=0.002). Baseline EZ-ALBI grade 2, 3, and serum alpha-fetoprotein >20 ng/ml were significantly associated with OS [hazard ratio (HR) 2.20 (95% confidence interval [CI] 1.24-3.88, p=0.007), 3.26 (95% CI 1.24-8.57, p=0.016), and 1.77 (95% CI 1.10-2.84, p=0.018), respectively). Following TACE, 42 (29.6%) patients had a worsening EZ-ALBI grade. However, the EZ-ALBI grade migration was not significantly correlated with OS. EZ-ALBI and ALBI score provided improved discriminatory ability (Harrell’s concordance index 0.599 and 0.602, respectively) compared with Child-Turcotte-Pugh and Model for End-stage Liver Disease scores.ConclusionsThe baseline EZ-ALBI score demonstrated good predictive performance for survival and a strong correlation with conventional ALBI scores. Both the EZ-ALBI and ALBI scores outperformed other prognostic models in patients with intermediate-stage HCC receiving TACE. However, the dynamic change in the EZ-ALBI grade after TACE was not associated with postprocedural survival.
Putative local adaptive SNPs in the genus Avicennia
Authors: Malekmohammadi Laleh , Sheidai Masoud , Ghahremaninejad Farrokh , Danehkar Afshin , Koohdar Fahimeh 
Published: March 2022

Abstract
The genus Avicennia with eight species grow in intertidal zones of tropical and temperate regions, ranging in distribution from West Asia, to Australia, and Latin America. These mangroves have several medicinal applications for mankind. Many genetic and phylogenetic studies have been carried out on mangroves, but none is concerned with geographical adaptation of SNPs. We therefore, used ITS sequences of about 120 Avicennia taxa growing in different parts of the world and undertook computational analyses to identify discriminating SNPs among these species and to study their association with geographical variables. A combination of multivariate and Bayesian approaches such as CCA, RDA, and LFMM were conducted to identify the SNPs with potential adaptation to geographical and ecological variables. Manhattan plot revealed that many of these SNPs are significantly associated with these variables. The genetic changes accompanied by local and geographical adaptation were illustrated by skyline plot. These genetic changes occurred not under a molecular clock model of evolution, and probably under a positive selection pressure imposed in different geographical regions in which these plants grow.
Oceanisphaera pacifica sp. nov., isolated from the intestine of Trichiurus japonicus
Authors: Xue Jian-Heng , Shi Li-Fang , Zhang Bei-Ning , Wu Wen-Jie , Gao Yuan , Zhu Qian , Zhao Li-Hua 
Published: March 2022

Abstract
A Gram-stain-negative, strictly aerobic, non-flagellated, oxidase- and catalase-positive, rod-shaped marine bacterium, designated strain DM8T, was isolated from the intestine of Trichiurus japonicus in Weihai, China. The strain optimally grew at 25-35℃, with 1.0-4.0% (w/v) NaCl and at pH 7.0-8.0. Its colonies were circular, slightly yellow, non-transparent, smooth, and approximately 0.8-1.5 mm in diameter, after being cultured for 48h on marine agar 2216. Based on the result of phylogenetic analysis of 16S rRNA gene sequence, strain DM8T had close relationship with Oceanisphaera profunda SM1222T (96.9%) and the similarity between DM8T and the type strain Oceanisphaera litoralis DSM 15406T was 94.7%. Genome sequencing revealed a genome size of 3,109,059 bp and a G+C content of 46.9 mol%. It had Q-8 as the sole respiratory quinone and possessed C16:0, summed features 3 (C16:1ω7c/C16:1ω6c) and summed features 8 (C18:1ω7c/C18:1ω6c) as major fatty acids. The major polar lipid profile was composed of phosphatidylglycerol and phosphatidylethanolamine. Based on the phenotypic, chemotaxonomic characterisations, phylogenetic properties and genome analysis, strain DM8T should represent a novel species of the genus Oceanisphaera, for which the name Oceanisphaera pacifica sp. nov. is proposed. The type strain is DM8T (=KCTC 82764T =MCCC 1K06133T).
Breastmilk feeding of infants at birth among people with confirmed SARS-CoV-2 infection in pregnancy — SET-NET, Five States, March 29, 2020–December 31, 2020
Authors: Lewis Elizabeth L. , Smoots Ashley N. , Woodworth Kate R. , Olsen Emily O’Malley , Roth Nicole M. , Yazdy Mahsa , Shephard Hanna , Sizemore Lindsey , Wingate Heather , Dzimira Paula , Reynolds Bethany , Lush Mamie , Fuchs Erika L. , Ojo Kristen , Siebman Sam , Hall Aron J. , Azziz-Baumgartner Eduardo , Perrine Cria , Hsia Jason , Ellington Sascha , Tong Van T. , Gilboa Suzanne M. 
Published: March 2022

Abstract
Objective
To describe prevalence of breastmilk feeding among people with SARS-CoV-2 infection during pregnancy and examine associations between breastmilk feeding, timing of maternal infection before delivery, and rooming-in status during delivery hospitalization.
Methods
Retrospective cohort using data from five states reporting initiation of breastmilk feeding of infants at birth among people with confirmed SARS-CoV-2 infection during pregnancy in 2020.
Results
Among 9760 (weighted N) people with SARS-CoV-2 infection in pregnancy, 86.0% (95% confidence interval [CI]: 83.3%-88.7%) initiated breastmilk feeding during birth hospitalization. People with infection ≤ 14 days before delivery had significantly lower prevalence of breastmilk feeding (adjusted prevalence ratio [aPR] 0.90, 95% CI: 0.82, 0.97) compared to those with infection > 14 days before delivery. When stratified by rooming-in status, the association between timing of infection and breastmilk feeding remained only among infants who did not room-in with their mother (aPR 0.78, 95% CI: 0.66, 0.92).
Conclusion
Pregnant and postpartum people with SARS-CoV-2 infection should be advised about the importance of breastmilk feeding, how to safely feed their infants in the same room, and have access to lactation support.
The role of pets in SARS-CoV-2 transmission: an exploratory analysis
Authors: Teixeira Ana Izabel Passarella , de Brito Raíssa Nogueira , Gontijo Carolina Carvalho , Romero Gustavo Adolfo Sierra , Ramalho Walter Massa , Haddad Rodrigo , Noronha Elza Ferreira , de Araújo Wildo Navegantes 
Published: March 2022

Abstract
Purpose
There is evidence that humans can transmit SARS-CoV-2 to cats and dogs. However, there is no evidence that they can transmit it back to humans or play any role in SARS-CoV-2 transmission. Here, we present an exploratory analysis on that matter.
Methods
We conducted a case control study with participants with flu-like symptoms seeking care at a primary healthcare unit to be tested for COVID-19. They were asked if they owned pet cats and/or dogs in their residences, and this variable was evaluated as exposure.
Results
The odds ratio of “having dogs and/or cats in the residence” was 1.29 (95%CI 1.08–1.54) of “having only dogs and no cats” was 1.26 (1.05–1.52), and “no dogs and only cats” was 1.29 (0.95–1.75).
Conclusion
Having a cat/dog in the house can affect the risk of infection by SARS-CoV-2.
Recent Advances in Utilizing Omics Approach to Identify the Bioactive
Peptides and Ripening Metabolism in Plant-based Food
Authors: Ee Kah Yaw , Lam Ming Quan , Chong Chun Shiong 
Published: March 2022

Abstract:
Bioactive peptides with potential health benefits and metabolic functionality have been
identified from plant-based food. The aim of this perspective is to report the recent progress in the
research of plant-derived bioactive peptides using the combination of omics technologies and
bioinformatics tools. Studies examining bioactive peptides with identified amino acid sequences and
well-characterized biological functionalities are highlighted. Various software, webtools and
workflows for analyzing and interpreting the biological data acquired from different omics
approaches are discussed. The emerging evidence from the integration of proteomics and
metabolomics data with advanced laboratory analytical methods supports more potential
applications in the envisioned development of nutraceutical and therapeutic products.
Notwithstanding, much works are mandatory to resolve those lied-ahead challenges before realizing
the proposed applications of plant peptides.
Unidentified N-glycans by N-glycosidase A were Identified by Nglycosidase
F under Denaturing Conditions in Plant Glycoprotein
Authors: Kim Jeongeun , Kim Jihye , Ryu Changsoo , Lee Jaeryong , Park Chi Soo , Jin Mijung , Kang Minju , Kim Ahyeon , Mun Chulmin , Kim Ha Hyung 
Published: March 2022

Background:
The identification of N-glycans in plant glycoproteins or plant-made pharmaceuticals is essential for understanding their structure, function, properties, immunogenicity, and allergenicity (induced by plant-specific core-fucosylation or xylosylation) in the applications of plant food, agriculture, and plant biotechnology. N-glycosidase A is widely used to release the N-glycans of plant glycoproteins because the core-fucosylated N-glycans of plant glycoproteins are hydrolyzed by N-glycosidase A but not by N-glycosidase F. However, the efficiency of N-glycosidase A activity on plant glycoproteins remains unclear.

Objective:
To elucidate the efficient use of N-glycosidases to identify and quantify the N-glycans of plant glycoproteins, the identification of released N-glycans by N-glycosidase F and their relative quantities with a focus on unidentified N-glycans by N-glycosidase A in plant glycoproteins, Phaseolus vulgaris lectin (PHA) and horseradish peroxidase (HRP), were investigated.

Methods:
Liquid chromatography–tandem mass spectrometry was used to analyze and compare the N-glycans of PHA and HRP treated with either N-glycosidase A or F under denaturing conditions. The relative quantities (%) of each N-glycan (>0.1%) to the total N-glycans (100%) were determined.

Results:
N-glycosidase A and F released 9 identical N-glycans of PHA, but 2 additional core-fucosylated N-glycans were released by only N-glycosidase A, as expected. By contrast, in HRP, 8 N-glycans comprising 6 core-fucosylated N-glycans, 1 xylosylated N-glycan, and 1 mannosylated N-glycan were released by N-glycosidase A. Moreover, 8 unexpected N-glycans comprising 1 core-fucosylated N-glycan, 4 xylosylated N-glycans, and 3 mannosylated N-glycans were released by N-glycosidase F. Of these, 3 xylosylated and 2 mannosylated N-glycans were released by only N-glycansodase F.

Conclusion:
These results demonstrated that N-glycosidase A alone is insufficient to release the N-glycans of all plant glycoproteins, suggesting that to identify and quantify the released N-glycans of the plant glycoprotein HRP, both N-glycosidase A and F treatments are required.