bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbial amelioration of salinity stress in endangered accessions of Iranian licorice (Glycyrrhiza glabra L.)
Authors: Mousavi Seyyed Sasan , Karami Akbar , Saharkhiz Mohammad Jamal , Etemadi Mohammad , Ravanbakhsh Mohammadhossein 
Published: July 2022

Abstract
Background
Glycyrrhiza glabra L. is a medicinal and industrial plant that has gone extinct due to different abiotic stress caused by climate change. To understand how the plant-associated microorganism can support this plant under salinity, we collected sixteen Iranian accessions of G. glabra L., inoculated their rhizomes with Azotobacter sp. (two levels, bacterial treatments, and no-bacterial treatments, and grown them under salinity stress (NaCl levels; 0, and 200 mM).

Results
Two accessions of Bardsir and Bajgah significantly showed higher resistant to salinity, for example by increasing crown diameter (11.05 and 11 cm, respectively) compared to an average diameter of 9.5 in other accessions. Azotobacter inoculation caused a significant increase in plant height and crown diameter. Among studied accessions, Kashmar (46.21%) and Ilam (44.95%) had the highest rate of membrane stability index (MSI). Evaluation of enzyme activity represented that bacterial application under salinity, increased polyphenol oxidase (PPO) (0.21 U mg−1 protein), peroxidase (POD) (3.09 U mg−1 protein U mg−1 protein), and phenylalanine ammonia-lyase (PAL) (17.85 U mg−1 protein) activity. Darab accession showed the highest increase (6.45%) in antioxidant potential compared with all studied accessions under Azotobacter inoculation. According to principal component analysis (PCA), it was found that the accession of Meshkinshahr showed a more remarkable ability to activate its enzymatic defense system under salt stress. Also, three accessions of Meshkinshahr, Eghlid, and Ilam were categorized in separated clusters than other accessions regarding various studied treatments.

Conclusion
Analysis indicated that five accessions of Meshkinshahr, Rabt, Piranshahr, Bardsir, and Kermanshah from the perspective of induced systematic resistance are the accessions that showed a greater morphophysiological and biochemical outcome under salinity. This study suggested that, inoculation of with Azotobacter on selected accession can relieve salt stress and support industrial mass production under abiotic condition.
Preconception vitamin D intake and obstetric outcomes in women using assisted reproductive technology: the Japan Environment and Children’s Study
Authors: Kyozuka Hyo , Murata Tsuyoshi , Fukuda Toma , Imaizumi Karin , Yamaguchi Akiko , Yasuda Shun , Suzuki Daisuke , Sato Akiko , Ogata Yuka , Hosoya Mitsuaki , Yasumura Seiji , Hashimoto Koichi , Nishigori Hidekazu , Fujimori Keiya ,   the Japan Environment, Children’s Study (JECS) Group, Kamijima Michihiro , Yamazaki Shin , Ohya Yukihiro , Kishi Reiko , Yaegashi Nobuo , Hashimoto Koichi , Mori Chisato , Ito Shuichi , Yamagata Zentaro , Inadera Hidekuni , Nakayama Takeo , Iso Hiroyasu , Shima Masayuki , Kurozawa Youichi , Suganuma Narufumi , Kusuhara Koichi , Katoh Takahiko 
Published: July 2022

Abstract
Background
In reproductive medicine, vitamin D (VitD) is of particular interest because its deficiency has been linked to various infertility issues. Thus, preconception care, including appropriate VitD supplementation, is essential, especially in women using assisted reproductive technology (ART). Despite the therapeutic benefits of VitD, adverse events due to a high daily intake may influence obstetric outcomes. However, the effects of either low or high preconception VitD intake on obstetric outcomes, including the outcomes in women who used ART, remain unclear. Therefore, the aim of this study was to examine the association between pre-pregnancy daily VitD intake and obstetric outcomes in Japanese women, including those who conceived through ART.

Methods
Data were obtained from the Japan Environment and Children’s study database comprising 92,571 women recruited between January 2011 and March 2014 in Japan. Participants were categorized into five quintiles according to pre-pregnancy VitD intake (Q1 and Q5 had the lowest and highest VitD intake, respectively) and stratified according to the use of ART. Multiple logistic regression was performed to identify the effects of pre-pregnancy VitD intake on preterm birth (PTB), low-birth weight infant (LBW), and small for gestational age (SGA).

Results
Using Q3 (middle VitD intake) as a reference, our analysis revealed that Q5 (highest VitD intake) showed an increased risk of LBW < 1500 g (adjusted odds ratio [aOR]: 1.09, 95% confidence interval [CI]: 1.00–1.18) and SGA (aOR: 1.26, 95% CI: 1.14–1.39) among women who conceived without ART. Among women who conceived with ART, we found that Q5 (highest VitD intake) showed an increased risk of PTB at < 37 weeks (aOR: 2.05, 95% CI: 1.27–3.31).

Conclusion
The present study revealed that higher VitD intake before pregnancy may affect perinatal outcomes, particularly in women using ART. Our findings may facilitate personalized preconceptional counseling regarding VitD intake based on the method of conception, especially among women using ART.
Neighbourhood and family correlates of immigrant children’s mental health: a population-based cross-sectional study in Canada
Authors: Sim Amanda , Georgiades Katholiki 
Published: July 2022

AbstractBackgroundImmigrant children exhibit significant variation in their mental health outcomes despite disproportionate exposure to socio-economic adversity compared to their non-immigrant peers. Identifying aspects of neighbourhood and family contexts that are most salient for immigrant children’s mental health can help to inform and target interventions to prevent mental disorder and promote mental well-being among this population.MethodsThe study analyzed multi-informant data from 943 first- and second-generation immigrant caregiver and child dyads from the Hamilton Youth Study, a representative sample of immigrant and non-immigrant families in Hamilton, Ontario. Multivariate multilevel regression models examined associations between neighbourhood and family characteristics and processes, and parent and child self reports of internalizing and externalizing problems.ResultsPositive and negative parenting behaviours were significantly associated with internalizing and externalizing problems, with negative parenting demonstrating associations with externalizing problems across both parent and child reports (b = 0.26–1.27). Neighbourhood social disorder and parental trauma exposure were associated with greater internalizing and externalizing problems, and neighbourhood immigrant concentration was associated with fewer externalizing problems for parent reports only. Adding parental distress and parenting behaviour to the models reduced the coefficients for parental trauma exposure by 37.2% for internalizing problems and 32.5% for externalizing problems and rendered the association with neighbourhood social disorder non-significant. Besides the parenting variables, there were no other significant correlates of child-reported internalizing and externalizing problems.ConclusionsResults highlight the importance of parenting behaviour and parental experiences of trauma and distress for immigrant children’s mental health. While not unique to immigrants, the primacy of these processes for immigrant children and families warrants particular attention given the heightened risk of exposure to migration-related adverse experiences that threaten parental and family well-being. To prevent or mitigate downstream effects on child mental health, it is imperative to invest in developing and testing trauma-informed and culturally responsive mental health and parenting interventions for immigrant families.
Persistent Aspergillus fumigatus infection in cystic fibrosis: impact on lung function and role of treatment of asymptomatic colonization—a registry-based case–control study
Authors: Blomquist Axel , Inghammar Malin , Al Shakirchi Mahasin , Ericson Petrea , Krantz Christina , Svedberg Marcus , Lindblad Anders , Påhlman Lisa I. 
Published: July 2022

Abstract
Background
Aspergillus fumigatus is the most common filamentous fungus isolated from the airways of people with cystic fibrosis (CF). The aim of this study was to investigate how chronic A. fumigatus colonization affects lung function in people with CF, to identify risk factors for colonization, and to evaluate antifungal treatment of asymptomatic Aspergillus colonization.

Methods
Data from 2014–2018 was collected from the Swedish CF registry and medical records. Baseline data before the start of A. fumigatus colonization was compared with the two succeeding years to evaluate how colonization and treatment affected lung function and other clinical aspects.

Results
A total of 437 patients were included, of which 64 (14.6%) became colonized with A. fumigatus during the study period. Inhaled antibiotics was associated with A. fumigatus colonization (adjusted OR 3.1, 95% CI 1.6–5.9, p < 0.05). Fungal colonization was not associated with a more rapid lung function decline or increased use of IV-antibiotics compared to the non-colonized group, but patients with A. fumigatus had more hospital days, a higher increase of total IgE, and higher eosinophil counts. In the Aspergillus group, 42 patients were considered to be asymptomatic. Of these, 19 patients received antifungal treatment. Over the follow up period, the treated group had a more pronounced decrease in percent predicted Forced Expiratory Volume in one second (ppFEV1) compared to untreated patients (− 8.7 vs − 1.4 percentage points, p < 0.05).

Conclusion
Inhaled antibiotics was associated with A. fumigatus colonization, but no association was found between persistent A. fumigatus and subsequent lung function decline. No obvious benefits of treating asymptomatic A. fumigatus colonization were demonstrated.
Putting measurement on a diet: development of a core set of indicators for quality improvement in the ICU using a Delphi method
Authors: Zegers Marieke , Verhage Rutger , Hesselink Gijs , van der Hoeven Johannes G. 
Published: July 2022

AbstractBackgroundThe number and efficacy of indicators used to monitor and improve the quality of care in Intensive Care Units (ICU) is debatable. This study aimed to select a consensus-based core set of indicators for effective quality improvement in the ICU.MethodsA Delphi study with a panel of intensivists, ICU nurses, and former ICU patients or relatives (n = 34) from general, teaching, and academic hospitals. Panelists completed a questionnaire in which they scored 69 preselected quality indicators on relevance using a nine-point Likert scale. Indicators were categorized using the rated relevance score into: ‘accepted, ‘equivocal’ and ‘excluded’. Questionnaire results were discussed in focus groups to reach consensus on the final set.ResultsResponse rates for the questionnaire and focus groups were 100 and 68%, respectively. Consensus was reached on a final set of 17 quality indicators including patient reported outcome measures (PROMs) and patient reported experience measures (PREMs). Other quality indicators relate to the organization and outcome of ICU care, including safety culture, ICU standardized mortality ratio, and the process indicator ‘learning from and improving after serious incidents’.ConclusionsICU clinicians and former patients and relatives developed a consensus-based core set of ICU quality indicators that is relatively short but comprehensive and particularly tailored to end-users needs.
Identification of genes and key pathways underlying the pathophysiological association between nonalcoholic fatty liver disease and atrial fibrillation
Authors: Chu Yanan , Yu Fangcong , Wu Yakui , Yang Jinxiu , Shi Jiaran , Ye Tianxin , Han Deheng , Wang Xingxiang 
Published: July 2022

Abstract
Background
Atrial fibrillation (AF) is one of the most prevalent sustained cardiac arrhythmias. The latest studies have revealed a tight correlation between nonalcoholic fatty liver disease (NAFLD) and AF. However, the exact molecular mechanisms underlying the association between NAFLD and AF remain unclear. The current research aimed to expound the genes and signaling pathways that are related to the mechanisms underlying the association between these two diseases.

Materials and methods
NAFLD- and AF- related differentially expressed genes (DEGs) were identified via bioinformatic analysis of the Gene Expression Omnibus (GEO) datasets GSE63067 and GSE79768, respectively. Further enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), the construction of a protein–protein interaction (PPI) network, the identification of significant hub genes, and receiver operator characteristic curve analysis were conducted. The gene-disease interactions were analyzed using the Comparative Toxicogenomics Database. In addition, the hub genes were validated by quantitative Real-Time PCR (qRT-PCR) in NAFLD cell model.

Results
A total of 45 co-expressed differentially expressed genes (co-DEGs) were identified between the NAFLD/AF and healthy control individuals. GO and KEGG pathway analyses revealed that the co-DEGs were mostly enriched in neutrophil activation involved in the immune response and cytokine-cytokine receptor interactions. Moreover, eight hub genes were selected owing to their high degree of connectivity and upregulation in both the NAFLD and AF datasets. These genes included CCR2, PTPRC, CXCR2, MNDA, S100A9, NCF2, S100A12, and S100A8.

Conclusions
In summary, we conducted the gene differential expression analysis, functional enrichment analysis, and PPI analysis of DEGs in AF and NAFLD, which provides novel insights into the identification of potential biomarkers and valuable therapeutic leads for AF and NAFLD.
GRB10 is a novel oncogene associated with cell proliferation and prognosis in glioma
Authors: Chen Yuanbing , Tang Miao , Xiong Jianbing , Gao Qiongjue , Cao Wuyang , Huang Jun 
Published: July 2022

Abstract
Background
Glioma is the most common malignant tumor of the central nervous system and is associated with a poor prognosis. This study aimed to explore the function of growth factor receptor-bound protein 10(GRB 10) in glioma.

Methods
The expression of GRB10 in glioma was determined based on the glioma transcriptome profile downloaded from The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA), and Gene Expression Omnibus (GEO) databases. RT-qPCR was performed to detect the expression of GRB10 in tissue samples obtained from 68 glioma patients. The patients were followed up via telephone or in-person outpatient visits to determine survival. Kaplan-Meier survival analyses were used to evaluate the effect of GRB10 on the prognosis of glioma patients. Further, we constructed GRB10 knockdown cell lines were constructed to investigate the effect of GRB10 on glioma. The cell growth, colony formation, cell cycle assay, EdU assay, and tumor formation in xenograft were performed.

Results
The expression level of GRB10 was positively correlated to the histological grades of gliomas. In addition, Kaplan-Meier survival curves revealed that glioma patients with lower expression of GRB10 had more prolonged survival. The knockdown of GRB10 was shown to inhibit cell proliferation, colony formation, and tumor formation in the xenograft models. Cell cycle assay revealed that the knockdown of GRB10 can inhibit the cells entering the G2/M phase from the S phase. The analysis of GSEA suggests that the expression of GRB10 was positively correlated with the hypoxia and EMT signaling pathway.

Conclusions
Our data revealed that GRB10 regulated tumorigenesis in glioma and played a vital role in promoting the glioma progression, which indicated that GRB10 could be used as a potential prognostic marker.
Effects of altitude and slope on the climate–radial growth relationships of Larix olgensis A. Henry in the southern Lesser Khingan Mountains, Northeast China
Authors: Qiao Jingjing , Sun Yujun 
Published: July 2022

Abstract
Background
The relationship between climate and radial growth of trees exhibits spatial variation due to environmental changes. Therefore, elucidation of how the growth–climate responses of trees vary in space is essential for understanding forest growth dynamics to facilitate scientific management with the ongoing global climate warming. To explore the altitudinal and slope variations of these interactions, tree-ring width chronologies of Larix olgensis A. Henry were analyzed in the southern Lesser Khingan Mountains, Northeast China.

Results
The radial growth of L. olgensis exhibited significant 5- to 10-year periodic changes at three altitudes and two slopes, and the frequency change occurred mainly during the early growth stage and after 2000. The radial growth of L. olgensis was significantly negatively correlated with September precipitation only at low altitudes, but also with the mean temperature in July–August and the mean maximum temperature in June–August at high altitudes. The radial growth of L. olgensis at low and middle altitudes as well as on the sunny slope led to a higher demand for moisture, while temperature was the key limiting factor at high altitudes and on the shady slope.

Conclusions
The climate–radial growth relationship of L. olgensis exhibits altitudinal and slope variability. This study quantitatively describes the spatially varying growth–climate responses of L. olgensis in the southern Lesser Khingan Mountains, which provides basic data for the management of L. olgensis forests and the prediction of future climate impacts on forest ecosystems.
Forage offering and seasonal intake comparisons to evaluate European rabbit threat in Nothofagus forests of southern Patagonia
Authors: Huertas Herrera Alejandro , Toro-Manríquez Mónica , Borrelli Laura , Lencinas María Vanessa , Martínez Pastur Guillermo 
Published: July 2022

AbstractBackgroundThe European rabbit (Oryctolagus cuniculusL. 1758) was introduced into different regions of the world, generating significant trade-offs that critically impacted native vegetation. Here, we evaluate the rabbit's forage intakes in three vegetation types (forests, shrublands, and grasslands) along the four seasons in a temperate forest landscape in Southern Patagonia and discuss the potential threats over native vegetation. We formulated the following questions: (i) what is the forage offer at each vegetation type? (ii) what is the rabbit's forage intake and how it varied across the seasons along the year? and (iii) which vegetation types and plant life forms were more used according to the rabbit's forage intakes?MethodsWe censused understory vegetation to characterize the forage offer at each vegetation type and determined seasonal dietary intakes using microhistological analysis of pellets. The plant species identified in the field were grouped according to life form classes (tree regeneration, shrubs, forbs, graminoids, orchids, ferns, bryophytes, and hemiparasites). Data were analysed through uni- and multi-variate analyses, determining relationships between forage offer and the rabbit's forage intakes.ResultsForage intakes revealed changes in plant life form consumption across vegetation types, where intake pressure was considerably different for tree regeneration (p = 0.001), graminoids (p = 0.001), and hemiparasites (p = 0.001). Besides, significant changes in consumption among seasons were detected for shrubs (p = 0.001), ferns (p = 0.030), and hemiparasites (p = 0.002). Although many species play an important role in the rabbit's forage intake networks (e.g.,Chiliotrichum diffusum,Holcus lanatus), the strongest intake linkages were found in exotic grasses (e.g.,Poa pratensisandFestucasp.), native hemiparasites (e.g.,Misodendrumsp.), native shrubs (e.g.,Empetrum rubrum), and native trees (e.g.,Nothofagussp.). The summer and autumn seasons presented higher intake compared to the winter and spring seasons. Furthermore, hemiparasites intake (e.g.,Misodendrumsp.) suggests that rabbits utilize different forage sources depending on the vegetation types.ConclusionsRabbits regularly inhabit the forest, even though it is not their preferred vegetation type to live. Rabbit management entails isolating regions that are more favourable to intake and emphasizing the year's season for rabbit control efforts.
A phase II pilot randomized controlled trial to assess the feasibility of the “supra-marginal” surgical resection of malignant glioma (G-SUMIT: Glioma supra marginal incision trial) study protocol
Authors: Mansouri Alireza , Lai Carolyn , Scales Damon , Pirouzmand Farhad 
Published: July 2022

Abstract
Background
High-grade gliomas are the most common primary malignant brain tumor in adults having a median survival of only 13–16 months. This is despite the current standard of maximal safe surgical resection followed by fractionated radiotherapy and chemotherapy. Extending the tumor resection limit beyond the gadolinium (GAD)-enhancing margin (i.e., supra-marginal resection) could in principle provide an added survival benefit as it has been shown that > 80% of post-operative tumor recurrence is within a 2-cm region surrounding the original GAD-enhancing margin. However, this must be weighed against the risk of potential damage to functional brain tissue.

Methods
In this phase II pilot randomized control trial (RCT), we aim to assess the feasibility of “supra-marginal” resection extending 1 cm beyond the enhancing tumor in adults with radiographic evidence of GAD-enhancing intra-axial tumor consistent with high-grade glioma in a safe anatomical location and a Karnofsky Performance Score > 60. With six academic institutions with established neurosurgical oncology practices in participation, we aim to enroll 72 patients over 2 years. Primary outcomes include evaluating the feasibility of performing a large-scale trial with regard to recruitment, allocation, and outcome documentation as well as safety data. Secondary outcomes include determining if there is an increased survival benefit with supra-marginal resection and impact on quality of life (Modified Rankin Scale (mRS), EuroQol-5D (ED-5D), 30-day all-cause mortality).

Discussion
Recent studies have revealed survival advantages comparing supra-marginal resection to standard attempt at gross total resection (GTR) with no additional perioperative surgical risk; however, the current quality of evidence is low and under-powered. Therefore, there are no current practice guidelines, and the philosophy of surgical resection is guided by individual surgeon preferences on an individual patient basis. This creates additional uncertainty and is potentially detrimental to our patients. This clinical equipoise supports the need for an adequately powered RCT to determine whether a supra-marginal resection can have a positive impact on survival for patients with HGGs. Our pilot RCT will test the feasibility of comparing the standard gross total resection of GAD-enhancing tumors and supra-marginal resection to prepare for a larger definitive multicenter RCT.

Trial registration
ClinicalTrials.gov, NCT04737577. Registered on February 4, 2021