bluuebunny/crossref_metadata_2025_split huggingface dataset:
An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging
Authors: Harada Tomoki , Hata Shoji , Fukuyama Masamitsu , Chinen Takumi , Kitagawa Daiju 
Published: June 2022

AbstractPhototoxicity is an important issue in fluorescence live imaging of light-sensitive cellular processes such as mitosis, especially in high spatiotemporal resolution microscopy that often requires high-intensity illumination. Among several approaches to reduce phototoxicity, the addition of antioxidants to the imaging media has been used as a simple and effective method. However, it remains unknown what are the optimal antioxidants that could prevent phototoxicity-induced defects during mitosis in fluorescence live cell microscopy. In this study, we analyzed the impact of phototoxicity on the mitotic progression in fluorescence live imaging of human diploid cells and performed a screen to identify the most efficient antioxidative agents that reduce it. Quantitative analysis shows that high amounts of light illumination cause various mitotic defects such as prolonged mitosis and delays of chromosome alignment and centrosome separation. Among several antioxidants known to reduce cellular phototoxicity, our screen reveals that ascorbic acid significantly alleviates these phototoxic effects in mitosis. Furthermore, we demonstrate that the addition of ascorbic acid to the imaging media enables fluorescence imaging of mitotic events at very high temporal resolution without obvious photodamage. Thus, this study provides a simple and practical method to effectively reduce the phototoxic effects on mitotic processes in fluorescence live cell imaging.
MLL family members regulate H3K4 methylation to ensure CENP-A assembly at human centromeres
Authors: Malik Kausika Kumar , Sridhara Sreerama Chaitanya , Lone Kaisar Ahmad , Katariya Payal Deepakbhai , Tyagi Shweta 
Published: June 2022

AbstractThe active state of centromeres is epigenetically defined by the presence of CENP-A interspersed with histone H3 nucleosomes. While the importance of dimethylation of H3K4 mark for centromeric transcription has been highlighted in various studies, the identity of the enzyme(s) depositing these marks on the centromere is still unknown. The MLL (KMT2) family play a crucial role in RNA polymerase II (Pol II)-mediated gene regulation by methylating H3K4. Here, we report that MLL family regulate transcription of human centromeres. CRISPR-mediated downregulation of MLL causes loss of H3K4me2, resulting in an altered epigenetic chromatin state of the centromeres. Intriguingly, our results reveal that loss of MLL, but not SETD1A, increases co-transcriptional R-loop formation, and Pol II accumulation at the centromeres. Finally we report that the presence of MLL and SETD1A is crucial for kinetochore maintenance. Altogether, our data reveals a novel molecular framework where both the H3K4 methylation mark and the methyltransferases regulate stability and identity of the centromere.
scPheno: A deep generative model to integrate scRNA-seq with disease phenotypes and its application on prediction of COVID-19 pneumonia and severe assessment
Authors: Zeng Feng , Kong Xuwen , Yang Fan , Chen Ting , Han Jiahuai 
Published: June 2022

AbstractCell-to-cell variability is orchestrated by transcriptional variations participating in different biological processes. However, the dissection of transcriptional variability in specific biological process at single-cell level remains unavailable. Here, we present a deep generative model scPheno to integrate scRNA-seq with disease phenotypes to unravel the invisible phenotype-related transcriptional variations. We applied scPheno on COVID-19 blood scRNA-seq to separate transcriptional variations in regulating COVID-19 host immunity and transcriptional variations in maintaining cell-type identity. In silico, we found CLU+IFI27+S100A9+ monocyte as the efficient cellular marker for the prediction of COVID-19 diagnosis. Inspiringly, using only 4 genes upregulated in CLU+IFI27+S100A9+ monocytes can predict the COVID-19 diagnosis of individuals from different country with an accuracy up to 81.3%. We also found C1+CD163+ monocyte and 8 C1+CD163+ monocyte-upregulated genes as the efficient biomarkers for the prediction of severity assessment. Overall, scPheno is an effective method in dissecting the transcriptional basis of phenotype variations at single-cell level.
Cardiac myosin binding protein-C palmitoylation is associated with increased myofilament affinity, reduced myofilament Ca2+ sensitivity and is increased in ischaemic heart failure
Authors: Main Alice , Milburn Gregory N. , Balesar Rajvi M. N. , Rankin Aileen C. , Smith Godfrey L. , Campbell Kenneth S. , Baillie George S. , van der Velden Jolanda , Fuller William 
Published: June 2022

AbstractCardiac myosin binding protein-C (cMyBP-C) is an essential regulator of cardiac contractility through its interactions with the thick and thin filament. cMyBP-C is heavily influenced by post-translational modifications, including phosphorylation which improves cardiac inotropy and lusitropy, and S-glutathionylation, which impairs phosphorylation and is increased in heart failure. Palmitoylation is an essential cysteine modification that regulates the activity of cardiac ion channels and soluble proteins, however, its relevance to myofilament proteins has not been investigated. In the present study, we purified palmitoylated proteins from ventricular cardiomyocytes and identified that cardiac actin, myosin and cMyBP-C are palmitoylated. The palmitoylated form of cMyBP-C was more resistant to salt extraction from the myofilament lattice than the non-palmitoylated form. Isometric tension measurements suggest c-MyBP-C palmitoylation reduces myofilament Ca2+ sensitivity, with no change to maximum force or passive tension. Importantly, cMyBP-C palmitoylation levels are reduced at the site of injury in a rabbit model of heart failure but increased in samples from patients with ischaemic heart failure. Identification of cMyBP-C palmitoylation site revealed S-glutathionylated cysteines C635 and C651 are required for cMyBP-C palmitoylation, suggesting an interplay between the modifications at these sites. We conclude that structural and contractile proteins within the myofilament lattice are palmitoylated, with important functional consequences for cardiac contractile performance.
How does family ownership and management influence green innovation of family firms: evidence from China
Authors: Yang Xuelei , Shang Hangbiao , Li Weining , Lan Hailin 
Published: June 2022

PurposeBased on the socio-emotional wealth and agency theories, this study empirically investigates the impact of family ownership and management on green innovation (GI) in family businesses, as well as the moderating effects of institutional environmental support factors, namely, the technological achievement marketisation index and the market-rule-of law index.Design/methodology/approachThis study empirically tests the hypotheses based on a sample of listed Chinese family companies with A-shares in 14 heavily polluting industries from 2009 to 2019.FindingsThere is a U-shaped relationship between the percentage of family ownership and GI, and an inverted U-shaped relationship between the degree of family management and GI. Additionally, different institutional environmental support factors affect these relationships in different ways. As the technological achievement marketisation index increases, the U-shaped relationship between the percentage of family ownership and GI becomes steeper, while the inverted U-shaped relationship between the degree of family management and GI becomes smoother. The market rule-of-law index weakens the U-shaped relationship between family ownership and GI.Originality/valueFirst, the authors enrich the research on the driving factors of GI from the perspective of the most essential heterogeneity of family businesses. This study shows nonlinear and opposite effects of family ownership and management on GI in family firms. Second, this study contributes to the literature on family firm innovation. GI, not considered by researchers, is regarded as an important deficiency in research on innovation in family businesses. Therefore, this study fills that gap. Third, the study expands research on moderating effects in the literature on GI from the perspective of institutional environmental support factors.
Intellectual structure of leadership studies in hospitality and tourism
Authors: Bavik Ali , Koseoglu Mehmet Ali 
Published: June 2022

PurposeThis study aims to investigate the intellectual structure of leadership research in the hospitality industry through citation, co-citation and heat map analysis.Design/methodology/approachSystematic bibliometric mapping was done using citation and co-citation analysis. This study covered journals from 1985 to 2020.FindingsAfter reviewing 172 published articles with 10,276 citations, results identified five main clusters.Practical implicationsHospitality managers can choose certain qualities (i.e. charisma, individualized consideration) or use servant leadership characteristics (i.e. selflessness) to direct employees toward more discretionary behavior.Originality/valueFormer holistic studies on leadership applied different approaches, such as review studies (i.e. systematic review and meta-analysis) or evaluative studies (e.g. productivity measures). It mainly focused on extending the understanding of different leadership types in tourism and hospitality. Nevertheless, relational studies (e.g. citation analysis, bibliographic analysis) remained untouched.
Individual differences, job demands and job resources as boundary conditions for relations between experienced incivility and forms of instigated incivility
Authors: Hughes Ian M. , Jex Steve M. 
Published: June 2022

Purpose
Using the job–demands resources model as a guide, this study aims to expand the understanding of the boundary conditions of the relation between experienced incivility and instigated incivility. The authors do so by focusing on the unique forms of instigated incivility: hostility, gossip, exclusionary behavior and privacy invasion. Drawing from past research, the authors focus on the personal resources of agreeableness and conscientiousness as individual difference boundary conditions, and the job demands and resources of workload and perceived emotional social support, respectively, as job-related boundary conditions.

Design/methodology/approach
The authors test their hypotheses using two-wave survey data collected from 192 customer service workers and hierarchical moderated multiple regression.

Findings
Analyses reveal that the relation between experienced incivility and gossip, a distinct type of instigated incivility, is stronger for those who are higher in agreeableness and perceived emotional social support, and weaker for those who report experiencing higher levels of workload.

Originality/value
This research advances knowledge on incivility by focusing on unique forms of instigated incivility, as opposed to instigated incivility broadly, as outcomes of experienced incivility. In doing so, this research adds nuance to recent findings surrounding the moderating role of personality in the experienced incivility and instigated incivility relation. The authors also report novel findings surrounding the influence of key job demands and resources.
Strategies implementing cash waqf system for Baitul Maal wat Tamwil to improve its commercial and social activities
Authors: Ascarya Ascarya , Masrifah Atika Rukminastiti 
Published: June 2022

Purpose
This study aims to devise policies in implementing cash waqf system of Baitul Maal wat Tamwil (BMT) in Indonesia, enabling the BMT to optimize its commercial and social activities to better achieve outreach, sustainability and welfare impact.

Design/methodology/approach
This study uses the strategic assumption surfacing and testing (SAST) method, with three groups of knowledgeable respondents, including expert, BMT practitioner and regulator to formulate important and certain policies.

Findings
The results show that four types of policies are required to improve cash waqf system of BMT, including 12 internal strategic policies, 15 internal operational policies, 15 external strategic policies and 9 external operational policies, which were found to be within a “certain planning region.” All of these policies have been agreed significantly by each group of respondents, as well as by all respondents combined. The most important-certain policies include Shiddiq, Amanah and professional Nazir, inculcate Islamic values to BMT employees and members, standard operating procedure and standard operating management of cash waqf management, technical assistance for Nazir to manage cash waqf and IT systems for BMT-cash waqf administration.

Research limitations/implications
The qualitative method used has its limitations, which could be improved by incorporating other methods. Moreover, the case and respondents are all Indonesian, so that the results are possibly only applicable to BMTs in Indonesia.

Practical implications
BMTs could adopt these policies in implementing their cash waqf management optimally.

Social implications
The management of cash waqf by BMT could help improve the social activities of the Baitul Maal directly from social cash waqf and indirectly from productive cash waqf.

Originality/value
To the best of the authors’ knowledge, this is the first study using SAST method to determine policies needed by the BMT to upgrade its cash waqf management producing more social programs for the society.
Review of work–life interface: a systematic literature analysis
Authors: Sharma Indu , Tiwari Vivek 
Published: June 2022

Purpose
The purpose of this study is to review the literature on work–life interface (WLI) studies conducted in India, including how cultural and institutional factors affect the work–life balance perception of Indian employees and how they differ from the west.

Design/methodology/approach
The preferred reporting items for systematic reviews and meta analyses (PRISMA) framework was used to select studies included in this systematic review.

Findings
Employees in India prioritize their family first because of the collectivist culture as opposed to the west, where work forms the role of root identity. Further, it was found that institutional support in India is lower than in developed western countries, resulting in difficulty for Indian employees to balance their work and life domains. Additionally, methodological advancement and large-scale multilevel research are much needed in India, as research on work–life studies is still in its infancy.

Research limitations/implications
This study is limited to qualitative synthesis of PRISMA framework. Adopting more advanced “meta-analysis” techniques to review quantitative studies could result in more comprehensive and informative results.

Originality/value
Since 2009, there has been an increase in empirical studies conducted on work–life studies in India, but no systematic review has been conducted to understand the various theories and variables used by researchers. In this study, we developed a conceptual model to increase the theoretical knowledge of WLI constructs.
The width and depth of literacies for tackling the COVID-19 infodemic
Authors: Koltay Tibor 
Published: June 2022

PurposeA wide choice of varied information and data-based tools is reviewed in order to determine their ability treating symptoms of the COVID-19 infodemic. Several literacies and derived literacies, presumably having the ability to fulfil these roles are enumerated. There is also a review of the impact of applying deconstruction, understanding, and anticipation as well as of tools for mitigating overload phenomena, and communication overload.Design/methodology/approachThe article reviews literacies deemed to promise reducing the impact of the information crisis, caused by the COVID-19 pandemic.FindingsA non-exhaustive review of literature, taken from sources of varied disciplines, resulting from reverse snowballing and forward citation mining confirmed that there is a wide choice of solutions from among literacies, derived literacies and other approaches that have the potential to combat annoyance and anxiety, caused by the infodemic.Originality/valueNo other, published research has looked at such a wide range of literacies and derived literacies, as well as other, related approaches linked them to the COVID-19 infodemic.
Information seeking amongst informal caregivers of people with dementia: a qualitative study
Authors: Hargreaves Sarah , Sbaffi Laura , Ford Nigel 
Published: June 2022

PurposeThis paper both supports previous findings relating to, and presents new insights into: the information needs and the information seeking processes of a sample of informal caregivers of people with dementia (in relation to their own needs and the interrelated needs of the people they are caring for); the extent to which such information needs are and are not being met; and the factors facilitating and hindering access to the right information.Design/methodology/approachThe study adopted a qualitative approach in the form of a thematic analysis of in-depth, semi-structured interviews with a sample of 20 informal caregivers from a range of different age groups, genders and caring roles.FindingsThematic analysis identified significant informational challenges, with a common perception that information seeking was onerous, requiring a proactive approach. Further challenges arose from a perceived lack of focus on carer needs coming up against the boundaries of professional knowledge and inconsistent information provision across the sample. Distance carers faced specific issues. A second theme of negative impacts described burdens arising from: difficulties in accessing information from a complex array of support services closure or change in services and unfulfilled information needs. Participants employed strategies to enable access to information, for example, being open about their caring role; and building formal or informal support networks. It is important to address emotional as well as cognitive dimensions of information needs.Practical implicationsThis research highlights a need for health and social care, practice and policy to acknowledge and address information needs of this diverse population and build resilience. Above all, information seeking and sharing must be understood within the context of the emotional impact of caring, and recognition of these twin needs is crucial.Originality/valueWhilst previous research has focussed on identifying specific needs and knowledge acquisition at cross-sections, a more holistic understanding of experiences is underexplored. This approach is needed to take into account broader contexts, diversity of experiences and different caring roles, e.g. primary and secondary carers, and in situ and distance carers.
Corporate governance mechanisms and corporate social responsibility disclosure: evidence from an emerging market
Authors: Ebaid Ibrahim El-Sayed 
Published: June 2022

PurposeThis study aims to examine the relationship between corporate governance mechanisms, namely, board independence, board size and gender diversity, and the extent of corporate social responsibility (CSR) disclosure for companies listed on the Saudi stock exchange.Design/methodology/approachData has been extracted from the annual reports of a sample of 67 companies listed on the Saudi Stock Exchange during the period 2014–2019. Three panel data techniques have been used to investigate the association between governance variables and the extent of CSR disclosures after statistically controlling the effects of the size, leverage and profitability of the companies.FindingsThe results of this study indicate that board independence and board size have positive and significant associations with the extent of CSR disclosures. However, the study finds that the percentage of female representation on the board has a positive effect on the extent of CSR disclosure, but that this effect is not statistically significant.Research limitations/implicationsThe results of this study are limited to the context in which the study was conducted, which is the Saudi stock exchange during the period 2014–2019, and then the generalization of the results may be limited to listed companies operating in a similar social and economic context. Also, the data sources in this study were limited to the annual reports of companies only.Practical implicationsThe results of this study provide some indications for policymakers in Saudi Arabia to take what is necessary to promote corporate governance mechanisms and, therefore, enhance CSR practices.Originality/valueThis study contributes to the literature on CSR by providing empirical evidence on the impact of corporate governance mechanisms on the extent of CSR disclosure from one of the developing countries, which is Saudi Arabia.
Financial intelligence unit of Pakistan: an evaluation of its performance and role in combating money laundering and terrorist financing
Authors: Sultan Nasir , Mohamed Norazida 
Published: June 2022

Purpose
This study aims to determine the performance of the Pakistani financial intelligence unit in combating money laundering/terrorist financing in local and global contexts.

Design/methodology/approach
The study used a qualitative research design. The objective is achieved by critically examining the Anti-money Laundering Act and its relevant clauses concerning the financial monitoring unit and other related legislation. Further, empirical data was collected through semi-structured interviews with chief compliance officers from regulated entities, regulators and premier law enforcer.

Findings
The performance of the financial monitoring unit has severe issues concerning the dissemination of financial intelligence due to its time taking behaviour, non-sharing of feedback with reporting agencies, dearth of international cooperation, lack of trained and relevant personnel and financial constraints.

Originality/value
To the best of the authors’ knowledge, this is a maiden study concerning financial monitoring unit in Pakistan.
Audit quality and COVID-19 restrictions
Authors: Gong Sabrina , Ho Nam , Jin Justin Yiqiang , Kanagaretnam Kiridaran 
Published: June 2022

Purpose
This study aims to examine declines in audit quality after the COVID-19 travel restrictions/stay-at-home orders were issued in the USA in early 2020.

Design/methodology/approach
Taking advantage of variation in the dates of stay-at-home orders issued by different US states, this study identifies engagements that were significantly affected by the lock down orders.

Findings
The results suggest that engagements affected by the restrictions produced lower audit quality, as measured through restatements and discretionary accruals, relative to those completed before COVID-19 travel restrictions/stay-at-home orders. Further analysis reveals that this decrease in audit quality was attributable to firms with high inventory relative to assets, high R&D expenses relative to assets and non-Big 4 auditors.

Practical implications
This study finds that the restrictions on physical and on-site interaction caused auditors to universally struggle with resource/judgment-intensive accounts such as inventory and R&D expenditures. The results suggest that while Big 4 auditors managed to maintain their status quo level of audit quality following COVID-19 restrictions, non-Big 4 auditors were unable to overcome the challenges of an online work environment and their audit quality declined.

Originality/value
To the best of the authors’ knowledge, this paper is the first to empirically examine changes in audit quality as a response to a substantial change in auditors’ working environment due to the global health crisis. As work-from-home becomes more prevalent in audit firms, the results suggest that, on average, this move does diminish audit quality.