bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insecticide Resistance Surveillance of Malaria and Arbovirus Vectors in Papua New Guinea 2017-2022
Authors: Katusele Michelle , Lagur Solomon , Endersby-Harshman Nancy , Demok Samuel , Goi Joelyn , Vincent Naomi , Sakur Muker , Dau Absalom , Kilepak Lemen , Gideon Stephen , Pombreaw Christine , Makita Leo , Hoffmann Ary , Robinson Leanne J , Laman Moses , Karl Stephan 
Published: May 2022

AbstractBackgroundInsecticide resistance monitoring is key for evidence-based control of Anopheles and Aedes disease vectors in particular, since the vast majority of insecticide-based public health adult vector control tools are reliant on pyrethroids. While widespread pyrethroid resistance in Anopheles species and Aedes aegypti has been described in many countries, data for Papua New Guinea are scarce. Available data indicate the local Anopheles populations remain pyrethroid-susceptible, making regular insecticide resistance monitoring even more important. Knowledge on Aedes insecticide resistance in PNG is very limited, however, high levels of Aedes aegypti resistance have been described. Here we present insecticide resistance monitoring data from across PNG generated between 2017 and 2022.MethodsMosquito larvae were collected in larval habitat surveys and through ovitraps. Mosquitoes were reared to adults and subjected to insecticide treated filter papers in WHO insecticide susceptibility bioassays. Subsets of Aedes mosquitoes were subjected to sequencing of the voltage-sensitive sodium channel (Vssc) region to identify resistance mutations.ResultsOverall, nearly 20,000 adult female mosquitoes from nine PNG provinces were used in the tests. We show that in general, Anopheline mosquitoes in PNG remain susceptible to pyrethroids but with worrying signs of reduced 24 h mortality in some areas. In addition, some Anopheles populations were indicated to be resistant against DDT. We show that Ae. aegypti in PNG are pyrethroid, DDT and likely bendiocarb resistant with a range of Vssc resistance mutations identified. We demonstrate that Ae. albopictus is DDT resistant and is likely developing pyrethroid resistance based on finding a low frequency of Vssc mutations.ConclusionThis study represents the largest overview of insecticide resistance in PNG. While Ae. aegypti is highly pyrethroid resistant, the Anopheline and Ae. albopictus populations exhibit low levels of resistance in some areas. It is important to continue to monitor insecticide resistance in PNG and prepare for the widespread emergence of pyrethroid resistance in major disease vectors.
Structure of V-ATPase from citrus fruit
Authors: Tan Yong Zi , Keon Kristine A. , Abdelaziz Rana , Imming Peter , Schulze Waltraud , Schumacher Karin , Rubinstein John L 
Published: May 2022

AbstractVacuolar-type ATPases (V-ATPases) are rotary proton pumps involved in numerous essential cellular processes in all eukaryotes. Difficulty in obtaining preparations of purified V-ATPase from plants in sufficient quantities for structural analysis has hindered determining the 3D structure of the plant V-ATPase. We used the Legionella pneumophila effector SidK to affinity-purify the endogenous V-ATPase from lemon fruit. The preparation was sufficient to partially cover an electron microscopy specimen grid, allowing structure determination for the enzyme in two rotational states. The structure defines the ATP:H+ ratio of the enzyme, demonstrating that it can establish a maximum ΔpH of ∼3, which is insufficient to maintain the low pH observed in the vacuoles of juice sac cells in lemons and other citrus fruit. Compared to the yeast and mammalian enzymes, the membrane region of the plant V-ATPase lacks subunit f and possesses an unusual configuration of transmembrane α helices. Subunit H, which inhibits ATP hydrolysis in the isolated catalytic region of V-ATPase, adopts two different conformations in the intact complex, hinting at a role in modulating activity in the intact enzyme.
Temporal changes in the microglial proteome of male and female mice after a diffuse brain injury using label-free quantitative proteomics
Authors: Doust Yasmine V. , Bindoff Aidan , Holloway Olivia G. , Wilson Richard , King Anna E. , Ziebell Jenna M. 
Published: May 2022

AbstractTraumatic brain injury (TBI) triggers neuroinflammatory cascades mediated by microglia, which promotes tissue repair in the short-term. These cascades may exacerbate TBI-induced tissue damage and symptoms in the months to years post-injury. However, the progression of the microglial function across time post-injury and whether this differs between biological sexes is not well understood. In this study, we examined the microglial proteome in the days (3- and 7-days) to 1 month (28 days) after a midline fluid percussion injury (mFPI) in male and female mice using label-free quantitative proteomics. We identified a reduction in microglial proteins involved with clearance of neuronal debris via phagocytosis at 3- and 7-days post-injury. At 28 days post-injury pro-inflammatory proteins were decreased and anti-inflammatory proteins were increased in microglia. These results indicate a reduction in microglial clearance of neuronal debris in the days post-injury with a shift to anti-inflammatory function by 1 month. The changes in the microglial proteome that occurred across time post-injury did not differ between biological sexes. However, we did identify an increase in microglial proteins related to pro-inflammation as well as insulin and estrogen signalling in males compared with female mice that occurred with or without a brain injury. Although microglial response was similar between males and females up to 1 month following TBI, biological sex differences in the basal microglial proteome has implications for the efficacy of treatment strategies targeting the microglial response post-injury.
MITE infestation of germline accommodated by genome editing in Blepharisma
Authors: Seah Brandon Kwee Boon , Singh Minakshi , Emmerich Christiane , Singh Aditi , Woehle Christian , Huettel Bruno , Byerly Adam , Stover Naomi , Sugiura Mayumi , Harumoto Terue , Swart Estienne Carl 
Published: May 2022

SummaryDuring a sophisticated developmental process, ciliates excise numerous internally eliminated sequences (IESs) from a germline genome copy, producing a functional somatic genome. Most IESs ultimately originate from transposons but homology is obscured by sequence decay. To obtain more representative perspectives on ciliate genome editing, we assembled forty thousand IESs of Blepharisma stoltei, from a much earlier-diverging lineage than existing models. Short IESs (< 115 bp) were largely non-repetitive, with a pronounced ~10 bp length periodicity, whereas longer IESs (max 7 kbp) were non-periodic and contained abundant interspersed repeats. Contrary to current models, the Blepharisma germline genome encodes few transposases. Instead, its most abundant repeat (8000 copies) was a Miniature Inverted-repeat Transposable Element (MITE), apparently a deletion derivative of a germline-limited Pogo-family transposon. We propose MITEs as an important and eventually self-limiting IES source. Rather than defending germline genomes against mobile elements, we argue that transposase domestication actually facilitates junk DNA accumulation.
A tunable broadband THz absorber using periodic arrays of graphene disks
Authors: Mohamad Khani Amir Ali , Aghaee Toktam , Mazloum Jalil , Jamali Morteza 
Published: May 2022

Purpose
A wide band perfect THz absorber is presented in this work. The structure includes two layers of graphene disks on the silicon dioxide dielectric layer while a golden plate is placed at the bottom to act as a fully reflecting mirror against THz waves. According to the simulations, the device is robust enough to show independent operation versus layers thicknesses variations, chemical potentials mismatches and changing of electron relaxation time. The designed THz absorber in this work is an appropriate basic block for several applications in THz optical systems such as sensors, detectors and modulators.

Design/methodology/approach
The layers in the proposed device are modeled via passive circuit elements and consequently, the equivalent circuit of the device is calculated. Leveraging the developed equivalent circuit model (ECM) and impedance matching concept, the proposed device is designed to perfect absorption with 4.7 THz bandwidth that possesses over 90% absorption. Ample simulations are performed using MATLAB (ECM) and CST (finite element method) to verify the superior performance of the device. According to the simulations, the device is robust enough to show independent operation versus layers thicknesses variations, chemical potentials mismatches and changing of electron relaxation time.

Findings
This work reports a wideband THz absorber, composed of two graphene layers. This paper considers the circuit model representation for two different layers of the device. For a unique structure, a highly tunable response versus chemical potential is obtained. The circuit model approach and impedance matching theory are exploited to reduce computational time regarding conventional approaches.

Originality/value
A wide band absorber in THz band is presented. Leveraging circuit model approach and impedance matching theory, the design procedure is simplified regarding CPU time and memory requirements compared to conventional methods. Detailed calculations and ample simulations verify the performance excellency of the device to absorb THz incident waves in 2–6.5 THz frequencies. Also, the robustness of the device is investigated versus parameters mismatches like layers thicknesses and chemical potentials values. According to the simulations and absorption response, the proposed device is an appropriate block to be used in THz optical systems such as detectors, imaging systems and optical modulators.
Identifying the risk factors and their interactions of human–robot collaboration implementation during engineering project construction: evidence from China
Authors: Chen Zhen , Zhao Yaqi , Zhou Xia , Hao Shengyue , Li Jin 
Published: May 2022

PurposeHuman–robot collaboration (HRC) is an emerging research field for the construction industry along with construction robot adoption, but its implementation remains limited in construction sites. This paper aims to identify critical risk factors and their interactions of HRC implementation during engineering project construction.Design/methodology/approachLiterature research, expert interviews, a questionnaire survey and a social network analysis (SNA) method were used. First, literature research and expert interviews were employed to identify risk factors of HRC implementation and preliminarily understand factor interactions. Second, a questionnaire survey was conducted to determine the degree of interactions between risk factors. Third, based on the data collected from the questionnaire survey, SNA metrics were used to find critical risk factors and critical interactions.FindingsThe critical risk factors consist of robot technology reliability, robot-perceived level, conflict between designers and users of construction robots, organisational culture, organisational strength, project cost requirements, changeability of project construction, project quality requirements and project safety requirements. The interactions between risk factors are strong and complex. Robot technology risk factors were relatively fundamental risk factors, and project risk factors had a direct influence on the risk of HRC implementation. The implementation cost of HRC was not identified as a critical risk factor. Individual risk factors could be mitigated by improving technical and organisational factors.Originality/valueThis paper contributes to the body of knowledge in the field of both HRC behaviours and its risk management in construction project management. Identifying the critical risk factors and their interactions of HRC implementation in the construction industry and introducing social network theory to the research on critical risk factors are the innovations of this paper. The findings and proposed suggestions could help construction professionals to better understand the HRC risk factors and to manage the risk of HRC implementation more effectively.
Contributions of universal school-based mental health promotion to the wellbeing of adolescents and preadolescents: a systematic review of educational interventions
Authors: Martínez-García Ariadna 
Published: May 2022

PurposeThe study aims to determine the contributions of universal school-based educational programs to the current and future worldwide youth mental health condition.Design/methodology/approachA systematic search was conducted in accordance with the PRISMA 2020 guidelines. Academic education and health databases including ERIC, Education Database, APA PsycInfo, APA PsycArticles, Psychology Database, and PubMed were used. Characteristics and outcomes of educational interventions developed in school settings and education potential for mental health promotion were examined.FindingsUniversal school-based mental health educational programs positively affect preadolescents and adolescents. The study review provided further insight into educational programs' characteristics and identified two primary curricula foci: mental health education and development of resiliency traits and skills.Originality/valueResearch on mental health promotion using a pedagogical approach is rare as most studies focus on mental health symptomatology and psychotherapy techniques training.
The determination of mode-I fracture toughness (by means of the Brazilian disc configuration) in the light of data provided by the 3D digital image correlation technique
Authors: Kourkoulis Stavros K. , Pasiou Ermioni D. , Markides Christos F. , Loukidis Andronikos , Stavrakas Ilias , Triantis Dimos 
Published: May 2022

PurposeThe determination of mode-I fracture toughness of brittle structural materials by means of the notched Brazilian disc configuration is studied. Advantage is taken of a recently introduced analytical solution and, also, of data provided by an experimental protocol with notched marble specimens under diametral compression using the loading device suggested by International Society for Rock Mechanics (ISRM) and also the three-dimensional digital image correlation (3D-DIC) technique.Design/methodology/approachThe analytical solution highlighted the role of geometrical factors, like, for example, the width of the notch, which are usually disregarded. The data of the experimental protocol were comparatively considered with those concerning the response of the specific material under uniaxial tensile load.FindingsThis combined study provided interesting data concerning some open issues, as it is the exact crack initiation point and the level of the critical load causing crack initiation. It was definitely indicated that the crack initiation point is not a priori known (even for notched specimens) and, also, that the maximum recorded load does not correspond by default to the critical load responsible for the onset of catastrophic macroscopic fracture.Originality/valueIt was suggested that the load considered critical one for the determination of mode-I fracture toughness KIC is erroneous. At a load equal to about 70% of the maximum one, a process zone is formed (zone of non-reversible phenomena) around the notch's crown, designating termination of the validity of any linear elastic solution used to determine the normalized stress intensity factors (SIFs). Moreover, at a load level equal to about 95% of the macroscopically observed fracture load, crack propagation has already begun. Therefore, the experimental procedure must be monitored with additional equipment, providing an overview of the displacement field developed during loading.
Geodesign and completeness indicators for urban areas: a transformative learning experience in urban planning teaching in Brazil
Authors: Moura Ana Clara Mourão , Rosa Ashiley Adelaide , Araújo Beatriz Maria Fernandes , Ferreira Felipe Andrade 
Published: May 2022

PurposeThis study aims to present a methodological experience using geodesign as a process and instrument that facilitates citizen awareness and the use of alternative urban parameters in a discipline of an undergraduate course in architecture and urbanism, about urban planning at a local scale.Design/methodology/approachAiming to develop solutions more suited to the reality of the area and attentive to contemporary practices of collaborative urban planning – for and with people – the methodological approach was divided into two complementary steps. The first step was elaborated through a general plan of ideas, with the aid of the Geodesign Hub platform, and for the more detailed second step, we used the Brazilian virtual GISColab geodesign platform. Both steps were conducted in workshop format.FindingsIn this experience, by incorporating geoinformation technology resources, geodesign proved to be a potential tool for creating opinions and decision-making regarding co-creative planning and experimenting with alternative urban parameters.Originality/valueIn the context of a current scenario of city growth oriented from the perspective of motor vehicles, the urban sprawl and in turn, the progressive loss of the human dimension in the urban space, students were introduced and encouraged to reflect on the different functions of the street and on the possibility of measuring urban quality from alternative parameters: completeness indicators.
Establishment and application of Whole Garment Knitted Skirt Template Library combining design and technology
Authors: Wang Jianping , Shen Deyao , Yao Xiaofeng , Lu Wenqin 
Published: May 2022

PurposeWhole garment knitwear is one of the knitwear development trends in the foreseeable future for its advantages of environmental friendliness and wearing comfort. However, the development of new styles of the whole garment knitwear requires both fashion design and computer programming, which makes it time-consuming and difficult. In this paper, a whole garment knitted skirt template Library is introduced to solve this problem.Design/methodology/approachThe template library composes of silhouette module, design element module and parts shaping technology module. It was built based on a comprehensive investigation of design and technology. By adhering to the principle of similarity and reusability, the template library of whole garment knitted skirts was established through the innovative design and hierarchical classification of compressed patterns and package patterns.FindingsWith the template library, more than 7.7 × 1025 package pattern templates can be generated through the permutation and combination of the package pattern templates of design elements and parts shaping technology.Originality/valueThe results indicated that it can accelerate the design process and improve the design efficiency of new styles with the template library. This approach can also provide inspiration for the designers and realize rapid response and personalized customization of knitted garment production. In addition, the whole garment templates can be applied into constructing other types of clothing template libraries, such as jackets, pants, etc.
Hope, tolerance and empathy: employees' emotions when using an AI-enabled chatbot in a digitalised workplace
Authors: Gkinko Lorentsa , Elbanna Amany 
Published: May 2022

PurposeInformation Systems research on emotions in relation to using technology largely holds essentialist assumptions about emotions, focuses on negative emotions and treats technology as a token or as a black box, which hinders an in-depth understanding of distinctions in the emotional experience of using artificial intelligence (AI) technology in context. This research focuses on understanding employees' emotional experiences of using an AI chatbot as a specific type of AI system that learns from how it is used and is conversational, displaying a social presence to users. The research questions how and why employees experience emotions when using an AI chatbot, and how these emotions impact its use.Design/methodology/approachAn interpretive case study approach and an inductive analysis were adopted for this study. Data were collected through interviews, documents review and observation of use.FindingsThe study found that employee appraisals of chatbots were influenced by the form and functional design of the AI chatbot technology and its organisational and social context, resulting in a wider repertoire of appraisals and multiple emotions. In addition to positive and negative emotions, users experienced connection emotions. The findings show that the existence of multiple emotions can encourage continued use of an AI chatbot.Originality/valueThis research extends information systems literature on emotions by focusing on the lived experiences of employees in their actual use of an AI chatbot, while considering its characteristics and its organisational and social context. The findings inform the emerging literature on AI.
Facilitating hotdesking in a hybrid campus environment: lessons from the hotdesking experiences of doctoral students in a US public university
Authors: Adikesavan Manju Aishwarya , Ramasubramanian Laxmi 
Published: May 2022

Purpose
University faculty, researchers and graduate students are increasingly working out of hotdesks, nonterritorial workspaces available on a “first come first served” basis and cleared of all work and personal possessions at the end of every work session. The aim of this study of the hotdesking experiences of doctoral students in a US public university facility is to identify the themes and coping behaviors associated with hotdesking and examine their implications for campus workspace design and policymaking.

Design/methodology/approach
This study uses multiple methods – direct and participant observation of flex workspaces in the study site, semi-structured interviews of doctoral students hotdesking in the study site and archival research of public institutional data.

Findings
Study participants work early or late to secure suitable hotdesks, perform important tasks in locations other than the study site, incur co-working space and home office costs, etc. to cope with the themes of uncertainty, lack of control and lack of workspace continuity associated with hotdesking. Workspace reservation systems, storage lockers and workspaces for diverse tasks can improve the on-campus hotdesking experience. Off-campus support such as financial support for setting up and maintaining a home office, subscription to co-working spaces, etc. can facilitate productivity and foster a sense of connection in hotdesk users.

Originality/value
This study contributes evidence that hotdesking doctoral students operate in a hybrid work environment composed of on- and off-campus locations. This study provides original insight that hotdesk users need on- and off-campus workspace support to experience productivity, connection and well-being in a hybrid campus work environment.