bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential oils of plants and their combinations as an alternative adulticides against Anopheles gambiae (Diptera: Culicidae) populations
Authors: Balboné Mahamoudou , Sawadogo Ignace , Soma Dieudonné Diloma , Drabo Samuel Fogné , Namountougou Moussa , Bayili Koama , Romba Rahim , Meda Georges Benson , Nebié Honorat Charles Roger , Dabire Roch K , Bassolé Imaël H N , Gnankine Olivier 
Published: May 2022

Abstract
The persistence of malaria and the increasing of resistance of Anopheles gambiae species to chemicals remain major public health concerns in sub-Saharan Africa. Faced to these concerns, the search for alternative vector control strategies as use of essential oils need to be implemented. Here, the five essential oils (EOs) from Cymbopogon citratus, Cymbopogon nardus, Eucalyptus camaldulensis, Lippia multiflora, Ocimum americanum obtained by hydro distillation were tested according to WHO procedures on Anopheles gambiae “Kisumu” and field strains collected in “Vallée du Kou”. Also, the binary combinations of C. nardus and O. americanum were examined. As results, among the EOs tested, L. multiflora was the most efficient regarding KDT50 and KDT95 and rate of morality values. Our current study showed that C8 (C.n 80% : O.a 20%) and C9 (C.n 90% : O.a 10%), were more toxic to Anopheles gambiae strain VK with the mortality rates reaching 80.7 and 100% at the 1% concentration, respectively. These two binary combinations shown a synergistic effect on the susceptible population. However, only C9 showed a synergistic effect on VK population. The bioactivity of the two EOs, Cymbopogon nardus and Ocimum americanum, was improved by the combinations at certain proportions and may constitute an alternative to pyrethroid resistance.
Hypertension and diabetes mellitus are associated with high FIB-4 index in a health checkup examination cohort without known liver disease
Authors: Sato Shunsuke , Kawai Hidehiko , Sato Sho , Iwasaki Hirohiko , Omori Masashi , Kita Yuji , Ikeda Yuji , Awatsu Takahito , Murata Ayato , Taniguchi Gentaro , Shimada Yuji , Genda Takuya 
Published: May 2022

Abstract
Background: Non-alcoholic fatty liver disease (NAFLD) is usually asymptomatic and lacks a specific biomarker; therefore, many individuals might remain undiagnosed even with advanced liver fibrosis. The aim of this study was to clarify the prevalence and clinical features of subjects with a high risk of advanced liver fibrosis in the general population, using the Fibrosis-4 (FIB-4) index.Methods: We retrospectively investigated 6,183 subjects without known liver disease who had participated in an annual health checkup examination. We analyzed the factors associated with high FIB-4 index (≥2.67) using a logistic regression analysis. Results: Among the 6,183 subjects, 76 (1.2%) had high FIB-4 index. Multivariate analysis identified hypertension (odds ratio [OR]=8.926; 95% confidence interval [CI] 4.031-19.768; P<0.001) and diabetes mellitus (OR=4.045; 95% CI 1.694-9.660; P=0.002) as important risk factors for high FIB-4 index. The rates of hypertension and diabetes mellitus in subjects with high FIB-4 index were 78.9% and 23.7%, respectively. No significant association was observed between obesity or large waist circumference and a high FIB-4 index. A history of cardiovascular disease was significantly more common in subjects with high FIB-4 index. These results were also observed in subjects with normal aminotransferase (ALT) levels. Conclusions: The present study revealed that approximately 1% of the general Japanese population has a high risk of advanced liver fibrosis. Many of these patients had hypertension and/or diabetes mellitus. Our findings suggest that there are many undiagnosed NAFLD patients with risk of advanced liver fibrosis in the general population.
IV segment portal vein reconstruction in split-liver transplantation with extended right grafts
Authors: Wang Dong , Fan Ning , Wang Xin , Sun Yandong , Guan Ge , Wang Jianhong , Zhu Xiaodan , Zang Yunjin , Cai Jinzhen , Guo Yuan 
Published: May 2022

Abstract
Background: Liver transplantation is one of the most effective treatment for end-stage liver disease. Split liver transplantation (SLT) can effectively improve the utilization efficiency of grafts . However, split liver transplantation still faces the shortcomings and not widely used in the surgery. How to improve the effective transplantation volume of split liver transplantation and promote the postoperative recovery of patients has important clinical significance.Methods: In our study, the donor liver was split into the extended right graft and left lateral sector, and the IV segment occure ischemia. In order to guarantee the functional graft size, and avoid the complications, we reconstructed the IV segment portal vein and left portal vein. And we analysed the operation time, intraoperative bleeding, liver function and postoperative complications.Results: In our research, 13 patients underwent IV segment portal vein reconstruction, and 8 patients did not undergo vascular reconstruction. We found that the ischemic area of the IV segment decreased significantly after IV segment portal vein reconstruction. We found that there was no significant difference in operation time and postoperative complications between the patients of the groups. There were significant differences in ALT on the first day and albumin on the sixth day after operation.Conclusion: It indicates that IV segment reconstruction in SLT surgery can alleviate the graft ischemic and obviously promote the recovery of liver function after operation. And, IV segment reconstruction as a novel operating procedure may be widely used in SLT.
Identification of tubulointerstitial genes and ceRNA networks involved in diabetic nephropathy via integrated bioinformatics approaches
Authors: Cao Haiyan , Rao Xiaosheng , Jia Junya , Yan Tiekun , Li Dong 
Published: May 2022

Abstract
Background: Diabetic nephropathy (DN) is the major cause of end-stage renal disease worldwide. The mechanism of tubulointerstitial lesions in DN is not fully elucidated. This article aims to identify novel genes and clarify the molecular mechanisms for the progression of DN through integrated bioinformatics approaches. Method: We downloaded microarray datasets from Gene Expression Omnibus (GEO) database and identified the differentially expressed genes (DEGs). Enrichment analyses, construction of Protein-protein interaction (PPI) network, and visualization of the co-expressed network between mRNAs and microRNAs (miRNAs) were performed. Additionally, we validated the expression of hub genes and analyzed the Receiver Operating Characteristic (ROC) curve in another GEO dataset. Clinical analysis and ceRNA networks were further analyzed. Results: Totally 463 DEGs were identified, and enrichment analyses demonstrated that extracellular matrix structural constituents, regulation of immune effector process, positive regulation of cytokine production, phagosome, and complement and coagulation cascades were the major enriched pathways in DN. Three hub genes (CD53, CSF2RB, and LAPTM5) were obtained, and their expression levels were validated by GEO datasets. Pearson analysis showed that these genes were negatively correlated with the glomerular filtration rate (GFR). After literature searching, the ceRNA networks among circRNAs/IncRNAs, miRNAs, and mRNAs were constructed. The predicted RNA pathway of NEAT1/XIST-hsa-miR-155-5p/hsa-miR-486-5p-CSF2RB provides an important perspective and insights into the molecular mechanism of DN. Conclusion: In conclusion, we identified three genes, namely CD53, CSF2RB, and LAPTM5, as hub genes of tubulointerstitial lesions in DN. They may be closely related to the pathogenesis of DN and the predicted RNA regulatory pathway of NEAT1/XIST-hsa-miR-155-5p/hsa-miR-486-5p-CSF2RB presents a biomarker axis to the occurrence and development of DN.
The effects of Cuprizone on murine subventricular zone-derived neural stem cells and progenitor cells grown as neurospheres
Authors: Molinari Yamila Azul , Byrne Agustin Jesus , Perez Maria Julia , Silvestroff Lucas , Franco Paula Gabriela 
Published: May 2022

Abstract
Despite the extensive use of the cuprizone (CPZ) demyelination animal model, there is little evidence regarding the effects of CPZ on a cellular level. Initial studies have suggested that oligodendrocytes (OL) are the main cell targets for CPZ toxicity. However, recent data have revealed additional effects on neural stem cells and progenitor cells (NSC/NPC), which constitute a reservoir for OL regeneration during brain remyelination. We cultured NSC/NPC as neurospheres to investigate CPZ effects on cell mechanisms which are thought to be involved in demyelination and remyelination processes in vivo. Proliferating NSC/NPC cultures exposed to CPZ showed overproduction of intracellular reactive oxygen species and increased progenitor migration at the expense of a significant inhibition of cell proliferation. Although NSC/NPC survival was not affected by CPZ in proliferative conditions, we found that CPZ treated cultures undergoing cell differentiation were more prone to cell death than controls. The commitment and cell differentiation towards neural lineages did not seem to be affected by CPZ, as shown by the conserved proportions of OL, astrocytes and neurons. Nevertheless, when CPZ treatment was performed after cell differentiation, we detected a significant reduction in the number and the morphological complexity of OL, astrogliosis and neuronal damage. We conclude that, in addition to damaging mature OL, CPZ also reduces NSC/NPC proliferation and activates progenitor migration. These results shed light on CPZ toxicity in the brain and could serve to understand the exhaustion of regenerative mechanisms from NSC/NPC in the chronic CPZ animal model.
Primary stability of different implant macrodesigns in a sinus floor elevation simulated model: An Ex Vivo Study
Authors: Imai Mikio , Ogino Yoichiro , DDS Hideaki Tanaka , Koyano Kiyoshi , Ayukawa Yasunori , DDS Takeshi Toyoshima 
Published: May 2022

Abstract
Background: A novel type of implant (Straumann® BLX implant) has been developed for certain stability from the mechanical and biological aspects and is expected for the implant placement in atrophic maxilla with sinus floor elevation (SFE). Purpose: The aim of this study was to evaluate the primary stability in the implants with different macrodesigns in a SFE simulated model. Primary stability defined as maximum insertion torque (MIT) and implant stability quotient (ISQ) were compared between this novel type of implant and other types.Materials and Methods: Five types of Straumann® 10mm length implants (Standard Plus; SP, Tapered Effect; TE, Bone Level; BL, Bone Level Tapered; BLT and BLX) and two types of Straumann® 6mm length implants (SP short, BLX short) were used in this study. Each Implants were inserted through 5 mm–thick porcine iliac crest blocks (a SFE simulated model). Primary stability was evaluated by using MIT and ISQ. Results: The mean values of MIT for BLX group showed significantly higher values than SP, BL (p < 0.01), and TE (p < 0.05) groups. The mean value of ISQ for BLX group was significantly higher than the other groups (p < 0.01). The mean value of MIT and ISQ for BLX and BLX short group were significantly higher than that for SP and SP short group (p < 0.01). Conclusions: In a SFE simulated ex vivo model, BLX group showed the highest values. These results suggest that implant selection can play a crucial role in the achievement of primary stability during SFE and simultaneous implant placement.
Regiospecific C-H Amination of (─)-Limonene into (─)-Perillamine by Multi-enzymatic Cascade Reactions
Authors: Ge Yue , Huang Zheng-Yu , Pan Jiang , Li Chun-Xiu , Zheng Gao-Wei , Xu Jian-He 
Published: May 2022

Abstract
Background
(−)-Limonene, one of cyclic monoterpenes, is an important renewable compound used widely as a key building block for the synthesis of new biologically active molecules and fine chemicals. (−)-Perillamine, as derived from (−)-limonene, is such a useful synthon for constructing more complicated and functionally relevant chemicals.
Aim
We aimed to report a more sustainable and more efficient method for the regiospecific C-H amination of (−)-limonene into (−)-perillamine.
Results
Here we report an artificial penta-enzymatic cascade system for the transformation of the cheap and easily available (−)-limonene into (−)-perillamine for the first time. This system is composed of cytochrome P450 monooxygenase, alcohol dehydrogenase and w-transaminase for the main reactions, as well as formate dehydrogenase and NADH oxidase for cofactor recycling. After optimization of the multi-enzymatic cascade system, 10 mM (−)-limonene was smoothly converted into 5.4 mM (−)-perillamine in a one-pot-two-step biotransformation, indicating the feasibility of multi-enzymatic C7-regiospecific amination of the inert C-H bond of (−)-limonene. This method represents a concise and efficient route for the biocatalytic synthesis of derivatives from similar natural products.
Method Development for the Quantitative Analysis of Multivitamin Supplements by Laser Ablation– Inductively Coupled Plasma–Mass Spectrometry (LA-ICP-MS)
Authors: Lopez Claudia Martinez , Carter Jake A. , Corzo Ruthmara , Todorov Todor I. 
Published: May 2022

Abstract
We present the development and optimization of an analytical method based on Laser Ablation–Inductively Coupled Plasma–Mass Spectrometry (LA-ICP-MS) for the analysis of multivitamin dietary supplements. Samples were ground and mixed with a cellulose powder containing internal standard elements; the resulting powder was pressed into pellets. Two calibration strategies were compared: (1) using increasing mass fractions of cellulose powder fortified with multiple elements of interest, and (2) matrix matching using a multivitamin reference material in addition to the multi-element cellulose powder. Laser and ICP-MS parameters were closely monitored to produce the best signal while reducing the variability between replicate measurements. An energy output of 0.5 J/cm2, a repetition rate of 30 Hz, and a He carrier gas flow rate of 800 mL/min provided the best compromise between sensitivity and reproducibility. These parameters produced the most similar ablation behavior between cellulose and a matrix-matched calibration strategy. The dissimilarities in ablation yield due to differences in focal points were successfully corrected after normalizing to an internal standard, resulting in relative differences of less than 10%. The results from the two different calibration approaches were compared to those obtained from solution ICP-MS and Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) analyses. For most multivitamins, both calibration methods (i.e., cellulose and matrix-matched calibrations) performed adequately for As, B, Co, Cr, Cu, Mg, Ni, Na, Pb, and Zn. Matrix-matched calibration additionally resulted in better recoveries for S and Fe. Overall, matrix-matched calibration resulted in better recoveries for more multivitamins in comparison with cellulose calibration.
FastIntegration: a fast and high-capacity version of Seurat Integration for large-scale integration of single-cell data
Authors: Chen Jinmiao , Li Mengwei , Zhang Xiaomeng , Siong Ang Kok 
Published: May 2022

Abstract
Atlas-scale integration of hundreds or even thousands of samples has become crucial for creating comprehensive cell maps. Here, we present FastIntegration which can integrate more than 4 million cells within 2 days. It outputs batch corrected values for all genes that we can use for downstream analyses. For the first time, we demonstrated that using batch corrected values can improve the performance of downstream analyses. In particular we demonstrated more accurately identified differentially expressed genes for cell types that are not shared between batches. Moreover, we also showed that FastIntegration outperforms existing methods for both homogeneous and heterogeneous data integration.
Investigation of Cytotoxic and Apoptotic Effects of Disodium Pentaborate Decahydrate on Ovarian Cancer Cells and Assessment of Gene Profiling
Authors: Gunel Nur Selvi , Yildirim Nuri , Ozates Neslihan Pinar , Okyay Latife Merve , Bagca Bakiye Goker , Sogutlu Fatma , Ozsaran Aydin , Avci Cigir Biray 
Published: May 2022

Abstract
After revealing the anti-cancer properties of boron, which is included in the category of essential elements for human health by the World Health Organization, the therapeutic potential of boron compounds has been begun to be evaluated, and its molecular effect mechanisms have still been among the research subjects. In ovarian cancer, mutations or amplifications frequently occur in the PI3K/Akt/mTOR pathway components, and dysregulation of this pathway is shown among the causes of treatment failure. In the present study, it was aimed to investigate the anti-cancer properties of boron-containing DPD in SKOV3 cells, which is an epithelial ovarian cancer model, through PI3K/AKT/mTOR pathway. The cytotoxic activity of DPD in SKOV3 cells was evaluated by WST-1 test, apoptotic effect by Annexin V and JC-1 test. The gene expressions associated with PI3K/AKT/mTOR pathway were determined by real-time qRT-PCR. In SKOV3 cells, the IC50 value of DPD was found to be 6.7 mM, 5.6 mM and 5.2 mM at 24th, 48th and 72nd hour, respectively. Compared with the untreated control group, DPD treatment was found to induce apoptosis 2.6-fold and increase mitochondrial membrane depolarization 4.5-fold. DPD treatment was found to downregulate PIK3CA, PIK3CG, AKT2, IGF1, IRS1, MAPK3, HIF-1, VEGFC, CAB39, CAB39L, STRADB, PRKAB2, PRKAG3, TELO2, RICTOR, MLST8, and EIF4B genes and upregulate TP53, GSK3B, FKBP8, TSC2, ULK1, and ULK2 genes. These results draw attention to the therapeutic potential of DPD, which is frequently exposed in daily life, in epithelial ovarian cancer and show that it can be a candidate compound in combination with chemotherapeutics.
Proteomics of small extracellular vesicle from human plasma for hepatocellular carcinoma
Authors: Dong Wei , Xia Zeyu , Chai Zehua , Qiu Zhidong , Wang Xuehong , Yang Zebin , Wang Junnan , Zhang Tingrui , Zhang Qinqin , Jin Junfei 
Published: May 2022

Abstract
Purpose
Liver cancer is one of the most common tumors with the seventh-highest incidence and the third-highest mortality. Many studies have shown that small extracellular vesicles (sEVs) play an important role in liver cancer. Here, we report comprehensive signatures for sEV proteins from hepatocellular carcinoma (HCC) patient plasma, which might be valuable for the evaluation and diagnosis of HCC.
Methods
We extracted sEVs from the plasma of controls and HCC patients. Differentially expressed proteins in the sEVs were analyzed via label-free quantification and bioinformatic analysis. Western blotting (WB) was used to validate the abovementioned sEV proteins that differentially accumulated in the validation cohorts.
Results
Protein expression profiles were performed for plasma sEVs from 21 HCC patients and 15 controls. Among 335 identified proteins in our study, 27 were significantly dysregulated, including 13 increased proteins that were involved predominantly in the complement cascade (C1QB, C1QC, C4BPA, and C4BPB) and the coagulation cascade (F13B, FGA, FGB, and FGG). We verified that the protein levels of C1QB, C1QC, C4BPA, and C4BPB were increased in the plasma sEVs of HCC patients in both the discovery cohort and validation cohort.
Conclusion
The complement cascade in sEVs was significantly involved in HCC progression. C1QB, C1QC, C4BPA, and C4BPB were highly abundant in sEVs of HCC patients' plasma, which might be potential molecular signatures.
Natural polymer nanocapsules containing eugenol for nectarine coating to control brown rot caused by Monilinia fructicola
Authors: Jacumazo Joslaine , Parchen Gabriela Pereira , Ballesteros-Garcia Meira Janete , Silva Nayana Cristina da , de Mio Louise Larissa May , Marques Francisco de Asssis , de Freitas Rilton Alves 
Published: May 2022

Abstract
The fruit infection by fungi reduces the quantity and quality of food for human consumption and causes economic damage. Thus, this study aimed to address the effects of nanocapsules (NCs) based on chitosan and carboxymethylcellulose containing eugenol in protecting nectarines against Monilinia fructicola, a brown rot agent, a worldwide important disease. NCs were prepared by Layer-by-Layer self-assembly starting from an anionic template and deposition with up to two polymeric layers. The sizes ranged from 158 nm (nanoemulsion) to 398 nm (two polymeric layers). In vitro release showed an initial burst followed by a gradual release. In addition to showing a good adherence to nectarine surface, the NCs coated with chitosan proved to be the best formulation for the control of brown rot in the antifungal test. Therefore, this study demonstrated that chitosan NCs containing eugenol could be an alternative to preserve fruit for longer periods in the post-harvest.
Mining-induced subsidence prediction by Displacement Discontinuity Method: A case from Barapukuria longwall coal mine, Bangladesh
Authors: Alam AKM Badrul , Fujii Yoshiaki , Eidee Shaolin Jahan , Boeut Sophea , Rahim Afikah Binti 
Published: May 2022

Abstract
It is essential to predict the mining-induced subsidence for sustainable mine management. The maximum observed subsidence having a noticeable areal extent due to Northern Upper Panels (NUP) and Southern Lower Panels (SLP) at the Barapukuria longwall coal mine is 5.8 m and 4.2 m, respectively, after the extraction of a 10 m thick coal seam. The mining-induced subsidence was simulated by the Displacement Discontinuity Method (DDM). The numerical model considered the effects of the ground surface, mining panels, faults, and the dyke. The predicted and the observed subsidence due to the mining of NUP and SLP were compared varying Young's modulus, and the 0.10 GPa Young's modulus was found to be the best match in the geo-environmental condition. The effects of the faults and the dyke in the calculation were negligible. Future subsidence was predicted by considering 30 m extraction of the thick coal seam as 15.7–17.5 m in NUP and 8.7–10.5 m in SLP. The vulnerable areas demarcated considering the tilt angle and extensile strain might extend up to the coal mine office area and some villages.