bluuebunny/crossref_metadata_2025_split huggingface dataset:
Description Quality of Sleep of Children With Autism
Authors: Kulsum Dewi Umu , Oktavia Oktavia 
Published: July 2022

Background: A developmental disorder that affects communication and behavior is known as Autism. The prevalence of autism is still increasing, ranging from 1-2/1000 in males more than in females (4:1). In children with autism, physical health problems are often complained of, one of which is sleep problems, such as delayed sleep onset, frequent awakenings at night, and sleep duration. This problem makes parents stressed because it impacts the severity of behavior both during the day and at night.
Objectives: The description of sleep quality in children with autism.
Methods: Cross-sectional approach to 55 special school students with autism in Cimahi. Data collection was collected by measuring sleep quality with the Children's Sleep Habits Questionnaire (CSHQ) with a cut-off point of < 30 good sleep quality and > 30 sleep problems with Chronbach's alpha = 0.68 to 0.78. Content validity; ROC = 0.41, differentiating between clinical and control groups; sensitivity = 0.80, specificity = 0.72. Data analysis was carried out univariate (frequency distribution) and bivariate with a chi-square test.
Results: It was found that most of the children with moderate Autism had sleep problems (54.5%) p = 0.005.
Conclusion: The study results describe that some children with autism in the city of Cimahi have sleep problems in conditions of delayed sleep onset and often wake up at night.
Application of Progressive Muscle Relaxation Therapy in Overcoming Nurse's Work Stress in Depok City Regional General Hospitals
Authors: Siregar Tatiana , Rabani Andal Izah 
Published: July 2022

Background: Every nurse who works in a hospital will face quite time-consuming and laborious work, especially when the bed occupancy rate (BOR) is 85%, and the work pressure of nurses is high. The effect of work stress manifests as physical fatigue, psychological changes, and behavioral changes. Nurses need to try to prevent work stress from happening for a long time by doing stress management, one of which is progressive muscle relaxation therapy.
Objectives: The purpose of the study was to determine the effect of progressive muscle relaxation therapy in reducing nurses’ work stress.
Methods: The study was conducted on five resident nurses who were trained using Progressive Muscle Relaxation and then used the Nursing Stress Scale to measure stress levels before and after training. The research design was Quasi Experiment with One group pretest and post-test designs; data analysis with paired t-test.
Results: The results showed that there was a significant effect of implementing progressive muscle relaxation therapy on reducing nurse work stress (p = 0.024; 95% CI).
Conclusion: There is a significant effect of progressive muscle relaxation therapy on the work stress response of nurses. Progressive muscle relaxation therapy is good to apply to hospital nurses who go through work stress so that stress does not occur which can risk reducing the quality of service to patients.
Surfactant-assisted alkaline pretreatment and enzymatic hydrolysis of Miscanthus sinensis for enhancing sugar recovery with a reduced enzyme loading
Authors: Cheng Xiyu , Luo Ying , Gao Yifan , Li Shen , Xu Chunming , Tang Shangyuan , Yang Yongkun , Zhang Zehua , Jiang He , Xu Hanli , Shi Shuobo , Yan Qiong 
Published: July 2022

Surfactants play a vital role in the delignification and saccharification of lignocellulosic biomass. A strategy for coupling surfactant-assisted alkaline pretreatment (SAP) with surfactant-assisted enzymatic hydrolysis (SEH) has been proposed for improving sugar recovery from a potential energy crop, Miscanthus sinensis. Poly (ethylene glycol) 2000 (PEG 2000) was found to be more efficient in SAP than in other tested surfactants. Compositional and structural analysis revealed that the SAP process with 1% of PEG 2000 produced more efficient lignin removal and microstructure disruption of the pretreated sample, thus indicating much higher reducing sugar yields of 544.4–601.2 mg/g compared to the samples that were untreated or pretreated by alkali alone. Moreover, SEH with 1% Tween 80, which could block the lignin-enzyme interactions, produced a substantial reduction of 33.3% in the enzyme loading to achieve a higher sugar recovery from the SAP sample.
Altered Regional Homogeneity in Patients With Diabetic Erectile Dysfunction: A Resting-State fMRI Study
Authors: Chen Jianhuai , Huang Xinfei , Tang Qinglai , Xiang Ziliang , Xu Yan , Liu Tao , Yang Zhaoxu , Yang Jie , Chen Yun 
Published: July 2022

IntroductionErectile dysfunction (ED) is a common complication of Type-2 Diabetes Mellitus (T2DM) for male patients and it is considered to be associated with different causes including hyperglicemia-induced vascular endothelial cell damages. However, the possible central neural mechanisms shared by these two diseases remain unclear. This study aimed to explore the changes of brain activity and their relationships with the clinical characteristics in patients with diabetic ED.MethodsThe data of resting-state functional magnetic resonance imaging were acquired in 31 T2DM patients with ED (DM-ED) and 31 matched healthy controls (HCs). The whole-brain regional homogeneity (ReHo) values were calculated and compared between groups. In addition, Pearson correlation analysis was performed to evaluate the relationships between brain regions with altered ReHo values and clinical characteristics in the patient group.ResultsThe DM-ED group exhibited increased ReHo values in the right middle frontal gyrus (orbital part) and decreased ReHo values in the left superior frontal gyrus (dorsolateral), paracentral lobule, precuneus and bilateral supplementary motor area when compared with the HCs group. Moreover, significantly negative correlations were found between ReHo values of the left superior frontal gyrus (dorsolateral) and IIEF-5 scores, as well as the level of HbA1c in the DM-ED group.ConclusionThe altered spontaneous brain activity in cognitive-related regions revealed by ReHo values might provide new insights into the neurological pathophysiology underlying DM-ED and serve as potential neuroimaging biomarkers for detecting and evaluating ED in diabetes patients.
An Individualized Recommendation for Controlled Ovary Stimulation Protocol in Women Who Received the GnRH Agonist Long-Acting Protocol or the GnRH Antagonist Protocol: A Retrospective Cohort Study
Authors: Chen Ming-Xing , Meng Xiang-Qian , Zhong Zhao-Hui , Tang Xiao-Jun , Li Tian , Feng Qian , Adu-Gyamfi Enoch Appiah , Jia Yan , Lv Xing-Yu , Geng Li-Hong , Zhu Lin , He Wei , Wan Qi , Ding Yu-Bin 
Published: July 2022

BackgroundThe GnRH agonist long-acting protocol and GnRH antagonist protocol are widely used in ovarian stimulation. Which protocol eliciting higher live birth rate for IVF/ICSI patients with different ages, different ovarian reserves and different body mass index (BMI) has not been studied. However, among these protocols, the one that elicits higher live birth in IVF/ICSI patients with different ages, ovarian reserves and body mass indexes (BMI) has not been identified.MethodsThis was a retrospective cohort study about 8579 women who underwent the first IVF-ET from January, 2018 to August, 2021. Propensity Score Matching (PSM) was used to improve the comparability between two protocols.ResultsAfter PSM, significant higher live birth rates were found in the GnRH agonist long-acting protocol compared to GnRH antagonist protocol (44.04% vs. 38.32%) (p&lt;0.001). Stratified analysis showed that for those with AMH levels between 3 ng/ml and 6 ng/ml, with BMI ≥ 24 kg/m2 and were aged ≥ 30 years old, and for those women with BMI &lt; 24kg/m2 and were aged ≥30 years whose AMH levels were ≤ 3ng/ml, the GnRH agonist long-acting protocol was more likely to elicit live births [OR (95%CI), 2.13(1.19,3.80)], [OR (95%CI), 1.41(1.05,1.91)]. However, among women with BMI ≥ 24kg/m2 and were aged ≥30 years whose AMH levels were ≤ 3ng/ml, the GnRH agonist long-acting protocol had a lower possibility of eliciting live births [OR (95%CI), 0.54(0.32,0.90)]. Also, among women with AMH levels between 3 ng/ml and 6 ng/ml, with BMI ≥ 24 kg/m2 and with age &lt; 30 years and for those with AMH levels between 3 ng/ml and 6 ng/ml, regardless of age, and with BMI&lt;24kg/m2,, the possibility of live births was similar between the two protocols [OR (95%CI), 1.06(0.60,1.89)], [OR (95%CI), 1.38(0.97,1.97)], [OR (95%CI), 0.99(0.72,1.37)]. Among the women with AMH levels ≤ 3 ng/ml and with were aged &lt; 30years, regardless of BMI, the possibility of live birth was similar between the two protocols [OR (95%CI), 1.02(0.68,1.54)], [OR (95%CI), 1.43(0.68,2.98)]. Moreover, among women with AMH levels ≥ 6ng/ml, the possibility of live birth was similar between the two protocols [OR (95%CI),1.42(0.75,2.69)], [OR (95%CI),1.02(0.19,5.35)], [OR (95%CI), 1.68(0.81,3.51)], [OR (95%CI), 0.51(0.10,2.55)].ConclusionsThe suitability of the GnRH agonist long-acting protocol or GnRH antagonist protocol to infertility patients is dependent on specific biological characteristics of the patients.
No bidirectional relationship between depression and periodontitis: A genetic correlation and Mendelian randomization study
Authors: Nolde Michael , Holtfreter Birte , Kocher Thomas , Alayash Zoheir , Reckelkamm Stefan Lars , Ehmke Benjamin , Baurecht Hansjörg , Baumeister Sebastian-Edgar 
Published: July 2022

BackgroundObservational and in-vivo research suggested a bidirectional relationship between depression and periodontitis. We estimated the genetic correlation and examined directionality of causation.MethodsThe study used summary statistics from published genome wide association studies, with sample sizes ranging from 45,563 to 797,563 individuals of European ancestry. We performed linkage disequilibrium score regression (LDSC) to estimate global correlation and used Heritability Estimation from Summary Statistics (ρ-HESS) to further examine local genetic correlation. Latent Heritable Confounder Mendelian randomization (LHC-MR), Causal Analysis using Summary Effect estimates (CAUSE), and conventional MR approaches assessed bidirectional causation.ResultsLDSC observed only weak genetic correlation (rg = 0.06, P-Value = 0.619) between depression and periodontitis. Analysis of local genetic correlation using ρ-HESS did not reveal loci of significant local genetic covariance. LHC-MR, CAUSE and conventional MR models provided no support for bidirectional causation between depression and periodontitis, with odds ratios ranging from 1.00 to 1.06 in either direction.ConclusionsResults do not support shared heritability or a causal connection between depression and periodontitis.
Autoimmune Atrophic Gastritis: The Role of miRNA in Relation to Helicobacter Pylori Infection
Authors: Zingone Fabiana , Pilotto Valentina , Cardin Romilda , Maddalo Gemma , Orlando Costanza , Fassan Matteo , Marsilio Ilaria , Collesei Eugenio , Pelizzaro Filippo , Farinati Fabio 
Published: July 2022

IntroductionMicroRNAs (miRNAs) have been proposed as diagnostic markers, biomarkers of neoplastic progression, and possible therapeutic targets in several immune-mediated diseases. We aimed to analyze the expression profile of selected miRNAs (miR21, miR142, miR223, miR155) in patients with autoimmune atrophic gastritis (AAG), patients with non-autoimmune multifocal atrophic gastritis (MAG), and healthy control subjects (HC).Materials and methodsA total of 103 patients with AAG were consecutively recruited for this study among those attending our gastroenterology outpatient clinic. Participating patients were divided into two groups: primary, not Helicobacter pylori (HP)-associated related AAG (n=57, P-AAG) and HP-associated AAG (n=46, HP-AAG); this subgroup included HP-positive patients, patients with previously reported HP infection, and patients harboring antral atrophy, considered as a stigma of HP infection. We also included 20 sex-age-matched MAG patients and 10 HC. Upper endoscopy with gastric biopsies were performed on each AAG and MAG patient. Circulating levels of miR21-5p, miR142-3p, miR223-3p, and miR155-5p were measured by RT-PCR in all groups.ResultsMiR-21 was over-expressed in P-AAG (p=0.02), HP-AAG (p = 0.04), and MAG (p=0.03) compared with HC. By contrast, miR-142 was more expressed in HC than in HP-AAG (p=0.04) and MAG (p=0.03). MiR-155 showed no significant differences among the four subgroups, while, unexpectedly, miR-223 was overexpressed in HC compared to P-AAG (p=0.01), HP-AAG (p=0.003), and MAG (p&lt;0.001), and was higher in P-AAG than in MAG (p=0.05).ConclusionsMiR-21 was over-expressed in patients with gastric precancerous conditions irrespective of etiology, while in the same subgroups miR-142 and miR-223 were under-expressed compared to healthy controls. Controlling miRNAs up- or downregulation could lead to a breakthrough in treating chronic autoimmune diseases and potentially interfere with the progression to cancer.
Microplastic prevalence in marine fish from onshore Beibu Gulf, South China Sea
Authors: Koongolla Jayamini Bimali , Lin Lang , Yang Chang-Ping , Pan Yun-Feng , Li Heng-Xiang , Liu Shan , Xu Xiang-Rong 
Published: July 2022

In recent years, microplastics have been widely detected in marine fish and may pose potential risks to fish and human health. Even though microplastic pollution is a critical issue, it represents as yet non-quantified threat for some context in the marine environment. In this study, 271 individuals comprising 32 species of marine fish collected from Beibu Gulf were examined for microplastic prevalence, with an aim to provide data on the abundance, physical classification, and chemical characterization of microplastics in the great bay along South China Sea. The results showed that the occurrence rate of microplastics was 93.7%, and the average abundance of microplastics was 1.02 ± 0.18 items per individual (ranging from 0.03 to 4.00 items per individual). Microplastic accumulation was observed with a great variation in different species, body sizes, living habitats, and feeding habits of marine fishes. The dominant polymers identified by μ-FTIR were polyethylene terephthalate and polypropylene. Fibers, smaller sizes (&lt;1 mm), and transparent color were the major characteristics of the detected microplastics, which might be important factors affecting the bioaccumulation. The present study revealed that microplastics in marine fish is a widespread issue in onshore Beibu Gulf.
Neurological Manifestations of SARS-CoV-2 Infection: Protocol for a Sub-analysis of the COVID-19 Critical Care Consortium Observational Study
Authors: Battaglini Denise , Premraj Lavienraj , Griffee Matthew , Huth Samuel , Fanning Jonathon , Whitman Glenn , Bastos Porto Diego , Arora Rakesh , Durham Lucian , Gnall Eric , Amato Marcelo , Williams Virginie , Noel Alexandre , De Franca Sabrina Araujo , Samoukovic Gordan , Pujo Bambang , Kent David , Marwali Eva , Al-Fares Abdulrahman , Stecher Stephanie-Susanne , Panigada Mauro , Giani Marco , Foti Giuseppe , Pelosi Paolo , Pesenti Antonio , White Nicole Marie , Li Bassi Gianluigi , Suen Jacky , Fraser John F. , Robba Chiara , Cho Sung-Min 
Published: July 2022

IntroductionNeurological manifestations and complications in coronavirus disease-2019 (COVID-19) patients are frequent. Prior studies suggested a possible association between neurological complications and fatal outcome, as well as the existence of potential modifiable risk factors associated to their occurrence. Therefore, more information is needed regarding the incidence and type of neurological complications, risk factors, and associated outcomes in COVID-19.MethodsThis is a pre-planned secondary analysis of the international multicenter observational study of the COVID-19 Critical Care Consortium (which collected data both retrospectively and prospectively from the beginning of COVID-19 pandemic) with the aim to describe neurological complications in critically ill COVID-19 patients and to assess the associated risk factors, and outcomes. Adult patients with confirmed COVID-19, admitted to Intensive Care Unit (ICU) will be considered for this analysis. Data collected in the COVID-19 Critical Care Consortium study includes patients' pre-admission characteristics, comorbidities, severity status, and type and severity of neurological complications. In-hospital mortality and neurological outcome were collected at discharge from ICU, and at 28-days.Ethics and DisseminationThe COVID-19 Critical Care Consortium main study and its amendments have been approved by the Regional Ethics Committee of participating sites. No further approval is required for this secondary analysis.Trial Registration NumberACTRN12620000421932.
Association Between Plasma Exosomes S100A9/C4BPA and Latent Tuberculosis Infection Treatment: Proteomic Analysis Based on a Randomized Controlled Study
Authors: Du Ying , Xin Henan , Cao Xuefang , Liu Zisen , He Yijun , Zhang Bin , Yan Jiaoxia , Wang Dakuan , Guan Ling , Shen Fei , Feng Boxuan , He Yongpeng , Liu Jianmin , Jin Qi , Pan Shouguo , Zhang Haoran , Gao Lei 
Published: July 2022

BackgroundIdentifying host plasma exosome proteins associated with host response to latent tuberculosis infection (LTBI) treatment might promote our understanding of tuberculosis (TB) pathogenesis and provide useful tools for implementing the precise intervention.MethodsBased on an open-label randomized controlled trial (RCT) aiming to evaluate the short-course regimens for LTBI treatment, plasma exosomes from pre- and post-LTBI treatment were retrospectively detected by label-free quantitative protein mass spectrometry and validated by a parallel reaction monitoring method for participants with changed or not changed infection testing results after LTBI treatment. Eligible participants for both screening and verification sets were randomly selected from the based-RCT in a 1:1 ratio by age and gender. Reversion was defined as a decrease in IFN-γ levels from &gt;0.70 IU/ml prior to treatment to 0.20 IU/ml within 1 week of treatment. The predictive ability of the candidate proteins was evaluated by receiver operating characteristic (ROC) analysis.ResultsTotally, two sample sets for screening (n= 40) and validation (n= 60) were included. Each of them included an equal number of subjects with persistent positive or reversed QuantiFERON-TB Gold In-Tube (QFT) results after LTBI. A total of 2,321 exosome proteins were detected and 102 differentially expressed proteins were identified to be associated with QFT reversion. Proteins with high confidence and original values intact were selected to be further verified. Totally, 9 downregulated proteins met the criteria and were validated. After verification, C4BPA and S100A9 were confirmed to be still significantly downregulated (fold change &lt;0.67,p&lt; 0.05). The respective areas under the ROC curve were 0.73 (95% CI: 0.57–0.89) and 0.69 (95% CI: 0.52–0.86) for C4BPA and S100A9, with a combined value of 0.78 (95% CI: 0.63–0.93). The positive and negative predictive values for combined markers were 70.10% (95% CI: 50.22–86.30%) and 55.63% (95% CI: 29.17–61.00%).ConclusionOur findings suggest that downregulated C4BPA and S100A9 in plasma exosomes might be associated with a host positive response to LTBI treatment. Further studies are warranted to verify the findings and potential underlying mechanisms in varied populations with a larger sample size.
Brain Activities Responding to Acupuncture at ST36 (zusanli) in Healthy Subjects: A Systematic Review and Meta-Analysis of Task-Based fMRI Studies
Authors: Huang Haoming , Yue Xiaomei , Huang Xi , Long Wenjie , Kang Shangyu , Rao Yawen , Zeng Jingchun , Zuo Junling , Wang Lin , Li Hongjuan , Wang Yeqing , Qiu Shijun , Zhao Weixuan 
Published: July 2022

PurposeStomach 36 (ST36, zusanli) is one of the important acupoints in acupuncture. Despite clinical functional magnetic resonance imaging (fMRI) studies of ST36 acupuncture, the brain activities and the neural mechanism following acupuncture at ST36 remain unclear.MethodsLiterature searches were conducted on online databases, including MEDLINE, Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure, Wanfang database, WeiPu database, and China Biology Medicine, for task-based fMRI studies of acupuncture at ST36 in healthy subjects. Brain regions activated by ST36 acupuncture were systematically evaluated and subjected to seed-based d mapping meta-analysis. Subgroup analysis was conducted on control procedures, manual acupuncture, electrical acupuncture (EA), and acupuncture-specific activations. Meta-regression analysis was performed to explore the effects of needle retention time on brain activities following ST36 acupuncture stimulation. The activated brain regions were further decoded and mapped on large-scale functional networks to further decipher the clinical relevance of acupuncturing at ST36.ResultsA total of sixteen studies, involving a total of 401 right-handed healthy participants, that satisfied the inclusion criteria were included in the present meta-analysis. Meta-analysis showed that acupuncturing on ST36 positively activates the opercular part of the right inferior frontal gyrus (IFG.R), left superior temporal gyrus (STG.L), and right median cingulate/paracingulate gyri (MCG.R) regions. Needle retention time in an acupuncture session positively correlates with the activation of the left olfactory cortex, as shown in meta-regression analysis. Subgroup analysis revealed that EA stimulation may be a source of heterogeneity in the pooled results. Functional network mappings showed that the activated areas were mapped to the auditory network and salience network. Further functional decoding analysis showed that acupuncture on ST36 was associated with pain, secondary somatosensory, sound and language processing, and mood regulation.ConclusionAcupuncture at ST36 in healthy individuals positively activates the opercular part of IFG.R, STG.L, and MCG.R. The left olfactory cortex may exhibit positive needle retention time-dependent activities. Our findings may have clinical implications for acupuncture in analgesia, language processing, and mood disorders.Systematic Review Registrationhttps://inplasy.com/inplasy-2021-12-0035.