bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toxicity and inhibition mechanism of gallic acid on physiology and fermentation performance of Escherichia coli
Authors: liu lina , Ma Xiaolong , Bilal Muhammad , Wei Linlin , Tang Shijie , Luo Hongzhen , Zhao Yuping , Wang Zhaoyu , Duan Xuguo 
Published: April 2022

Abstract
Gallic acid is a natural phenolic acid that has a stress inhibition effect on Escherichia coli. This study elucidates the physiological mechanism of E. coli 3110 response to gallic acid by integrating fermentation characteristics and transcriptional analysis. Compared with the control (without stress), the cell growth was severely inhibited, and irregular cell morphology appeared in the case of high levels of gallic acid stress. With the increase of gallic acid content in the fermentation medium, the glucose consumption of E. coli decreased successively. Moreover, cofactor levels (ATP, NAD+ and NADH) of E. coli 3110 were also lowered under gallic acid after 20 h stress, indicating a stronger inhibitory effect of gallic acid on E. coli. The transcriptional analysis revealed that gallic acid altered the gene expression profiles related to five notable differentially regulated pathways. The genes related to the two-component system were up-regulated, while the genes associated with ABC-transporter, energy metabolism, carbon metabolism, and fatty acid biosynthesis were down-regulated. This is the first report on the systematic evaluation of the toxicity of gallic acid on E. coli. This study has a guiding significance for the efficient production of phenolic compounds by E. coli and provides new ideas for the study of microbial tolerance to environmental stress and the discovery of related tolerance targets.
A VIKOR-based group decision-making approach to software reliability evaluation
Authors: Yue Chuan 
Published: April 2022

Abstract
Software reliability evaluation is important to attribute of software quality. A new group decision-making (GDM) is provided to evaluate software reliability, where the model is based on an extended VIsekriterijumska optimizacija i KOmpromisno Resenje (VIKOR) technique. The individual utility and individual regret in general VIKOR technique are extended to group utility and group regret. A specific regret matrix is provided in new VIKOR-based GDM method. A new ranking method is provided in a static environment. Another new ranking method is provided in a dynamic environment. To implement a user-based evaluation for software reliability, the picture fuzzy set is used for handling the questionnaire information. It is noted that the existing projection measure is not always reasonable in picture fuzzy setting. To solve this problem, a new normalization projection measure is provided in picture fuzzy setting. And a new GDM method is established for software reliability evaluation. The feasibility and practicability developed method in this work are illustrated by an experimental analysis.
Clinical significance and molecular characteristics of circulating plasma cells in multiple myeloma
Authors: Xia Yuan , Xu Min , Zhang Jue , Zhang Run , Jin Yuanyuan , Wu Yujie , Shi Qinglin , Li Jianyong , Chen Lijuan 
Published: April 2022

Abstract
Multiple myeloma (MM) is one of the most common hematological malignancies and remains incurable to date. Clonal plasma cells can be detected in the peripheral blood of most MM patients, and previous studies have shown an association between the quantification of circulating plasma cells (CPC) and prognosis. However, the significance of CPC for response assessment and prognosis prediction as well as the mechanism underlying CPC formation in Chinese population has not been fully elucidated. In this investigation, we used multi-parameter flow cytometry (MFC) for CPC quantification and next-generation sequencing (NGS) technology for mutational landscape mapping to identify the correlation of CPC level with clinical characteristics and the mutations. As a result, we demonstrated that CPC quantification could effectively mirror the tumor load, and CPC≥0.105% at diagnosis or detectable CPC after therapy indicates poor treatment response and adverse outcome, and it is verified that the introduction of CPC level into the R-ISS system enables a more accurate risk stratification. We also noticed an elevated percentage of light-chain MM in patients with high CPC, this is the first time, to the best of our knowledge, to report the subtype pattern regarding CPC. Mutational landscape revealed that TP53 mutations and pathways involving chromatin or chromosome regulation and adhesion may be potential mechanisms accounting for CPC formation. Accordingly, the detection of CPC by MFC may provide a less-invasive, convenient and, more importantly, reliable approach for risk stratification and disease monitoring in Chinese population.
Proof-of-concept Study to Estimate Individual Post-Therapy Dosimetry in Men with Advanced Prostate Cancer Treated with 177Lu-PSMA I&amp;T Therapy
Authors: Xue Song , Gafita Andrei , Dong Chao , Zhao Yu , Tetteh Giles , Menze Bjoern H , Ziegler Sibylle , Weber Wolfgang , Afshar-Oromieh Ali , Rominger Axel , Eiber Matthias , Shi Kuangyu 
Published: April 2022

Abstract
It is still debating if individualized dose should be applied for the emerging PSMA-targeted radionuclide therapy (RLT). A critical consideration in this debate is the necessity and feasibility of individual estimation of post-therapy dosimetry before the treatment. In this study, we aimed to prove the concept of individual dosimetry prediction based on pre-therapy imaging and laboratory measurements. Methods: 23 patients with metastatic castration-resistant prostate cancer (mCRPC) treated with 177Lu-PSMA-I&T RLT were included retrospectively. Included patients had available pre-therapeutic 68Ga-PSMA-HEBD-CC PET/CT and at least 3 planar and 1 SPECT/CT dosimetry imaging. Overall, 43 cycles of 177Lu-PSMA I&T RLT were applied. Organ-based standard uptake value (SUV) uptake was obtained from pretherapy PET/CT scans. Patient individual dosimetry was calculated for kidney, liver, spleen, and salivary glands using Hermes Hybrid Dosimetry 4.0 from the post-treatment 177Lu-PSMA I&T imaging studies. Machine learning methods were explored for individual dose prediction from PET images. The accuracy of these dose predictions was compared with the accuracy of population-based dosimetry estimates. Mean absolute percentage error was used to assess the prediction error of estimated dosimetry. Results: An optimal machine learning method achieved a dosimetry prediction error of 15.8 ± 13.2% for kidney, 29.6%±13.7% for liver, 23.8%±13.1% for salivary glands and 32.1 ± 31.4% for spleen. In contrast, the prediction based on literature population mean has significantly larger error (p < 0.01), 25.5 ± 17.3% for kidney, 139.1%±111.5% for liver, 67.0 ± 58.3% for salivary glands, and 54.1 ± 215.3% for spleen. Conclusion: The preliminary results confirmed the feasibility of individual estimation of post-therapy dosimetry before the RLT and its added value to empirical population-based estimation. The exploration of individual dose prediction may support the identification of the role of treatment planning for RLT.
The antidepressant effects of Atorvastatin are mediated by reduction of nitric oxide pathway in ovariectomized mice
Authors: Jahanabadi Samane Jahanabadi , Safshekan Pegah Safshekan , Moradi Ali Moradi 
Published: April 2022

Abstract
Atorvastatin has been associated with reduced depressive-like behavior and nitric oxide (NO) has been suggested to play a major role in the pathogenesis of some neurodegenerative diseases, including depression. Hormonal fluctuations throughout ovariectomy, postpartum and menopause contribute to the higher incidence of depression in women. We therefore investigated the antidepressant-like effects of atorvastatin and the possible role of nitric oxide pathways in ovariectomized (OVX) mice.To this end, female mice were undergone bilateral ovariectomy and various doses of atorvastatin were injected alone and combined with non-specific NO synthase inhibitor (L-NAME) or an NO precursor (L-arginine). Behavioral changes were recorded in the open-field test (OFT), forced swim test (FST) and Tail Suspension Test (TST). Additionally, hippocampal levels of nitrite, a major metabolite of NO, were determined. Seven days following the procedure, OVX mice showed significantly prolonged immobility time in comparison with the sham group. Atorvastatin (0.1 and 1 mg/kg) exerted antidepressant-like effects in OVX mice and combination of sub-effective dose of atorvastatin (0.01 mg/kg) with a sub-effective dose of L-NAME (10mg/kg) resulted in a robust antidepressant-like effect in OVX mice. NO precursor L-arginine (750 mg/kg) suppressed the antidepressant-like effect of a potent dose of atorvastatin (0.1 mg/kg) in OVX mice. None of the treatments altered the locomotor activity of mice in OFT. Moreover, the increased hippocampal nitrite concentrations caused by ovariectomy were also prevented by atorvastatin administration.The study demonstrated that atorvastatin exerts antidepressant-like effects of in OVX mice through a mechanism probably involving suppression of nitric oxide pathway.
TTYH3, a potential prognosis biomarker associated with immune infiltration and immunotherapy response in lung cancer
Authors: Wei Zimeng , Zeng Xingruo , Lei Yufei , He Hengjing , Jamal Muhammad , Zhang Chengjie , Tan Haiyan , Xie Songping , Zhang Qiuping 
Published: April 2022

Abstract
Purpose
The recognition of new diagnostic and prognostic biological markers for lung cancer is an essential and eager study. It’s shown that ion channels play important roles in regulating various cellular processes and have been suggested to be associated with patient survival. However, TTYH3, as a novel maxi-Cl− channel, its role in lung cancer remains elusive.
Methods
The expression, diagnosis and prognostic efficacy of TTYH3 were analyzed by public databases and clinical samples. Cell functional experiments used to explore the effects of TTYH3 on cell ability. GO and KEGG enrichment analysis revealed pathway that TTYH3 and its co-expressed genes were enriched in. TIMER and R language analyses were used to detect the correlation between TTYH3 and immune cell infiltration and the immunotherapy response.
Results
TTYH3 was found up-regulated in lung cancer tissues compared to normal tissues and possessed a prominent diagnostic and prognostic value. Knockdown TTYH3 significantly inhibited the proliferation of lung cancer cells. Enrichment analyses results showed that TTYH3 and its co-expressed genes were mainly involved in immune related signaling pathways. Further investigation clarified that TTYH3 had a positive correlation with the infiltration of macrophage, T cell regulatory, CD4+ T cell and high TTYH3 expression indicated worse immunotherapy response and shorter survival after immune checkpoint blockade treatment.
Conclusion
This study not only deciphers the diagnostic and prognostic value of TTYH3 but also provides TTYH3-based estimation of immunotherapy response for lung cancer patients, which might provide new strategies like anti-TTYH3 combined with immune therapy for the treatment of lung cancer.
Adapalene synergistically with doxorubicin promotes apoptosis of TNBC Cells by hyperactivation of the ERK1/2 pathway through ROS induction
Authors: Mehraj Umar , Mir Irfan Ahmad , Hassan Mahboob ul , Mir Nissar Ahmad , Mir Manzoor Ahmad 
Published: April 2022

Abstract
Purpose: Doxorubicin (DOX) is a commonly used chemotherapeutic agent to treat several malignancies, including aggressive tumors like triple negative breast cancer (TNBC) lacking hormonal receptors and HER2 amplification. It has a limited therapeutic index owing to its extreme toxicity and the emergence of drug resistance. As a result, there is a pressing need to find innovative drugs that enhance the effectiveness of doxorubicin while minimizing its toxicity. Recently, the third-generation retinoid adapalene (ADA) was reported as an effective anticancer agent in several malignancies. This study aimed to determine the anticancer activity of ADA in TNBC cells and its effectiveness in combination with DOX and the mechanistic pathways in inhibiting tumorigenicity.Methods: Cell viability was evaluated using the 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium (MTT) and pharmacodynamic interactions (synergism, antagonism, or additivity) by Chou-Talalay's method. Flow cytometry and fluorimetry were used to determine apoptosis, cell cycle and reactive oxygen species (ROS). Western blotting was utilized to analyze the molecular mechanism and apoptotic protein levels.Results: We found that ADA in synergistic manner with doxorubicin inhibits growth, colony formation, and migration of TNBC cells. Moreover, ADA as single agent or in combination with DOX resulted in ROS accumulation triggering hyperphosphorylation of Erk1/2 and activating caspase 3 and apoptosis. N-acetyl-L-cysteine (NAC) pre-treatment inhibited ROS accumulation triggered by DOX-ADA and substantially reduced Erk1/2 phosphorylation.Conclusions: Our results demonstrate that ADA is a potent antitumor agent that may be used as a single agent or combined with present therapeutic regimens for TNBC treatment.
Gross Tumor Volume Delineation in Primary Prostate Cancer on 18 F-PSMA-1007 PET/MRI and 68 Ga-PSMA-11 PET/MRI
Authors: Zhang Yan-Nan , Lu Zhen-Guo , Wang Shuai-Dong , Lu Xin , Zhu Lei-Lei , Yang Xu , Fu Li-Ping , Zhao Jun , Wang Hai-Feng , Xiang Zuo-Lin 
Published: April 2022

Abstract
Purpose: We aimed to probe the clinical value of 18F-PSMA-1007 and 68Ga-PSMA-11 PET/MRI in the GTV delineation of radiotherapy for prostate cancer (PCa). Methods: 69 patients were retrospectively enrolled (57 in 18F subgroup and 12 in 68Ga subgroup). First, three physicians delineated the GTVs by visual method and threshold method with thresholds of 30%, 40%, 50%, and 60% SUVmax. The volume correlation and differences in GTVs were assessed. Second, the DSC was applied to estimate the spatial overlap between GTVs. Then we also evaluated the association between DSCs and clinical parameters (including age, the reference GTV-MRI, PSA, Gleason score, risk classification, and SUVmax). Last, the interobserver variability was evaluated. GTV-MRI was set as a reference. Results: In 18F subgroup: (1) GTV-PET/MRI and GTV-PET60% had significant correlation and difference with the reference GTV-MRI (p<0.05). (2) DSC between GTV-MRI and GTV-PET/MRI was>0.7. (3) The GTV-MRI (R2=0.462, p<0.05) was the influencing factor of the DSC, and positively affected the DSC. In 68Ga subgroup: (1) GTV-PET/MRI, GTV-PET30% had significant correlation and difference with the reference GTV-MRI (p<0.05). (2) DSC between GTV-MRI and GTV-PET/MRI were>0.7. (3) There was a significant correlation between GTV-MRI (r=0.580, p<0.05) and the DSC. There were no significant differences in tumor volume delineated by different observers in both subgroups. Conclusion: It is feasible to visually delineate GTV on PSMA PET/MRI in PCa radiotherapy, we emphasize the GTV delineation on the PET/MRI fusion image. In addition, the overlap degree was the highest between GTV-MRI and GTV-PET/MRI, and it increased with increasing volume.
WITHDRAWN: Assessment of Genetic Diversity and Population Genetic Structure of Santalum album L. in India by Genic and Genomic SSR markers
Authors: Fatima Tanzeem , Srivast Ashutosh , Hanur Vageeshbabu S. , Srinivasa M. 
Published: April 2022

Abstract

Sandalwood (Santalum album L.) is a highly valued aromatic tropical tree. It is known for its high quality heartwood and oil. In this study, 39 genic and genomic SSR markers were used to analyze the genetic diversity and population structure of 177 S. album accessions from 14 populations of three states in India. High genetic diversity was observed in terms of number of alleles 127, expected heterozygosity (He) ranged from 0.63-0.87 and the average PIC was 0.85. The selected population had relatively moderate genetic diversity with Shannon’s information index (I) >1.0 followed by 0.02 (mean coefficient of genetic differentiation) FST. AMOVA revealed that 92% of the variation observed within accessions. Based on cluster and Structure result, individuals were not clustered as per their geographical origin. Furthermore the clusters were clearly distinguished by principal component analysis and the result revealed that PC1 reflected the moderate contribution in genetic variation (6%) followed by PC2 (5.5%). From this study, moderate genetic diversity and genetic differentiation was observed in S. album populations. The genetic results are of great significance for scientifically selection of superior genotypes and germplasm conservation to promote the tree improvement of S. album populations.
The risk factors and outcomes for radiological abnormalities in early convalescence of COVID-19 patients caused by the SARS-CoV-2 Omicron variant in Tianjin, China: a retrospective, multicenter follow-up study
Authors: Wang Hong , Yang Qingyuan , Li Fangfei , Wang Huiying , Yu Jing , Ge Xihong , Gao Guangfeng , Xia Shuang , Xing Zhiheng , Shen Wen 
Published: April 2022

Abstract
Background: The emergence of the SARS-CoV-2 Omicron variant has been triggering the new wave of COVID-19 globally. However, the risk factors and outcomes for radiological abnormalities in the early convalescent stage (1 month after onset) of Omicron infected patients are still unknown.Methods: Patients were retrospectively enrolled if they were admitted to the hospital due to COVID-19 and underwent chest CT scans during hospitalization between 8 January and 24 February 2022. The chest CTs and clinical data obtained at admission and 1 month after onset were longitudinally analyzed. Spearman rank correlation analysis was used to investigate the correlation between laboratory markers and CT scores. Uni-/multi-variable logistic regression tests were performed to explore independent risk factors for radiological abnormalities at admission and residual pulmonary abnormalities after 1 month. Results: We assessed 316 COVID-19 patients, including 47% with radiological abnormalities at admission and 23% with residual pulmonary abnormalities at 1-month follow-up. In a multivariate regression analysis, age ≥ 50 years, body mass index ≥ 23.87, days after vaccination ≥ 81 days, lymphocyte count ≤ 1.21×10-9/L, Interleukin-6 ≥ 10.05 pg/mL and IgG ≤ 14.140 S/CO were independent risk factors for CT abnormalities at admission (odds ratio = 2.870, 4.171, 2.360, 2.826, 3.886 and 2.828, respectively; P < 0.05 for each comparison). The presence of interlobular septal thickening and IL-6 ≥ 5.85 pg/mL were the independent risk factors for residual pulmonary abnormalities at 1-month follow-up (odds ratio = 3.619 and 3.197, respectively; P < 0.05 for each comparison). There were no significant changes in the number of involved lung lobes (P = 0.308) and total CT score (P = 0.068) during the early convalescent stage. And the level of IL-6 showed positive correlation with CT score at admission (spearman r = 0.202, P = 0.030).Conclusions: The higher IL-6 level was a common independent risk factor for CT abnormalities at admission and residual pulmonary abnormalities at 1-month follow-up. And there were no obvious radiographic changes during the early convalescent stage in patients with residual pulmonary abnormalities.
Application of Columba livia Feather as a Bioindicator of Industrial Pollution
Authors: Asgari Fariba , Sajjadi Nooshin , Zaeimdar Mojgan , Sadeghi Mostafa , Tehrani Mahnaz Mirza Ebrahim 
Published: April 2022

Abstract
In developing countries, due to the development of industrial technologies and heavy metals pollution in the environment, the use of bioindicators such as bird feathers could be a good way to transmit pollutants to the environment. In this study, the accumulation of heavy metals such as lead, zinc and cadmium in Columba livia feathers in males and females and live and dead species in Industrial town of Zinc-Zanjan and comparison with control species outside industrial town were studied. The feathers of 164 pigeons were collected and dissolved by chemical digestion method. In this study, heavy metals were measured by the avanta GBC atomic absorption device. The mean concentrations of lead, zinc and cadmium were determined greater in dead female pigeons, dead male pigeons, live female pigeons, and live male pigeons respectively. This was also the case with the control species. Statistical analysis of data was performed with SAS software (Statistical Analysis System). (P ≤ 0.05, 95% confidence level) and (P ≤ 0.01, 99% confidence level) were considered as significant differences. The results of statistical tests in the samples inside Industrial town of Zinc-Zanjan showed that there is a significant statistical relationship between gender (male-female) and live and dead bird and elements of lead, zinc and cadmium, while for the control species were different. Significant levels of heavy metals in bird feathers indicate their usefulness as a non-invasive tool to show the concentration of metals in pigeons to control the level of pollution in the environment. The present study showed that Columba livia had suitable biological characteristics for use as a bioindicator of industrial pollution.
Core community persistence despite dynamic spatiotemporal responses in the associated bacterial communities of farmed Pacific oysters
Authors: King Nathan , Smale Dan , Thorpe Jamie , McKeown Niall , Andrews Adam , Browne Ronan , Malham Shelagh 
Published: April 2022

Abstract
A breakdown in host-bacteria relationships have been associated with the progression of a number of marine diseases and subsequent mortality events. For the Pacific oyster, Crassostrea gigas, Summer Mortality Syndrome (SMS) is one of the biggest constraints to growth of the sector and is set to expand into temperate systems as ocean temperatures rise. Currently, a lack of understanding of natural spatiotemporal dynamics of the host-bacteria relationship limits our ability to develop microbial-based monitoring approaches. Here, we characterised the associated bacterial community of C. gigas, at two Irish oyster farms, unaffected by SMS, over the course of a year. We found C. gigas harboured spatiotemporally variable bacterial community that were distinct from bacterioplankton in surrounding seawater. Whilst the majority of bacteria-oyster associations were transient and highly variable, we observed clear patterns of stability in the form of a small core consisting of six persistent Amplicon Sequence Variants (ASVs). This core made up a disproportionately large contribution to sample abundance (34 ± 0.14 %), despite representing only 0.034% of diversity across the study, and have been associated with healthy oysters in other systems. Overall, our study demonstrates the consistent features of oyster bacterial communities across spatial and temporal scales and provides an ecologically meaningful baseline to track environmental change.