bluuebunny/crossref_metadata_2025_split huggingface dataset:
Valorisation of Basa fish (Pangasius bocourti) waste: Oil extraction and encapsulation
Authors: Debbarma Ashika , Kumar Vivekanand , Mishra Prashant K. , Kakatkar Aarti S. , Gautam Raj Kamal , Umra Farhat , Chatterjee Suchandra 
Published: October 2022

SummaryFish waste is a source of various nutraceuticals and bioactive compounds such as polyunsaturated fatty acids (PUFAs), peptides, vitamins and minerals. This waste can be utilised for the extraction of fish oil for the preparation of value‐added supplements for humans. In this study, oil was extracted from wastes (bones, head and viscera) of Basa (Pangasius bocourti) fish and encapsulated with alginate as a coating material using the simple extrusion process. In total fatty acid composition, the most predominant was monounsaturated fatty acid (MUFA) (30.74–61.67%), followed by saturated fatty acid (SFA) (26.45–38.22%) and PUFA (7.03–21.01%) in different sections. Alginate concentration (2.5%) with oil loading of 30% showed higher emulsion stability with the least pore space, hence, considered optimum for encapsulation of oil. The encapsulated beads were stable up to 12 days as determined by peroxide value (18.67 ± 1.15 meq Kg−1), acid value (14.13 ± 0.35 mg KOH g−1) and free fatty acid value (8.42 ± 0.51%) when stored at room temperature.
Selection and validation of reference genes for qPCR analysis of differentiation and maturation of THP‐1 cells into M1 macrophage‐like cells
Authors: Ren Guoqiang , Juhl Morten , Peng Qiuyue , Fink Trine , Porsborg Simone Riis 
Published: October 2022

AbstractFor cell‐based assays studying monocytes and macrophages, the immortalized monocyte cell line THP‐1 is widely used and stimulated with phorbol 12‐myristate 13‐acetate, lipopolysaccharide (LPS) and/or interferon‐γ (IFN‐γ), after which it differentiates and polarizes into proinflammatory M1‐like macrophages. For the quantification of this and the effect of different factors affecting these processes, the expression levels of various maturation markers are determined using reverse transcription‐quantitative PCR. For this purpose, stably expressed reference genes are crucial. However, no studies evaluating the stability of reference genes in THP‐1 cells stimulated with LPS and IFN‐γ have been performed. Therefore, this paper describes the selection of the most used reference genes [RPL37A (ribosomal protein L37a), GAPDH (glyceraldehyde‐3‐phosphate dehydrogenase), UBC (ubiquitin C), B2M (0β2‐microbulin), ACTB (β‐actin) and PPIA (cyclophilin A)], the in silico primer design, the analysis and the validation of these in accordance with the MIQE (Minimum Information for Publication of Quantitative Real‐Time PCR Experiments) guidelines and more recent recommendations for the validation of the stability of reference genes. Using the RefFinder platform, including the four most popular algorithms for reference gene validation, the Delta CT, BestKeeper, NormFinder and geNorm, we find the reference genes GAPDH and UBC to be the most stable. Furthermore, we demonstrate that the normalization of gene expression data using the least stable reference genes, ACTB and B2M, dramatically affects the interpretation of experimental data. Taken together, it is vital to validate the stability of reference genes under the specific experimental conditions used when utilizing the THP‐1 monocyte model system.
The role of neutrophils in trained immunity
Authors: Kalafati Lydia , Hatzioannou Aikaterini , Hajishengallis George , Chavakis Triantafyllos 
Published: October 2022

SummaryThe principle of trained immunity represents innate immune memory due to sustained, mainly epigenetic, changes triggered by endogenous or exogenous stimuli in bone marrow (BM) progenitors (central trained immunity) and their innate immune cell progeny, thereby triggering elevated responsiveness against secondary stimuli. BM progenitors can respond to microbial and sterile signals, thereby possibly acquiring trained immunity‐mediated long‐lasting alterations that may shape the fate and function of their progeny, for example, neutrophils. Neutrophils, the most abundant innate immune cell population, are produced in the BM from committed progenitor cells in a process designated granulopoiesis. Neutrophils are the first responders against infectious or inflammatory challenges and have versatile functions in immunity. Together with other innate immune cells, neutrophils are effectors of peripheral trained immunity. However, given the short lifetime of neutrophils, their ability to acquire immunological memory may lie in the central training of their BM progenitors resulting in generation of reprogrammed, that is, “trained”, neutrophils. Although trained immunity may have beneficial effects in infection or cancer, it may also mediate detrimental outcomes in chronic inflammation. Here, we review the emerging research area of trained immunity with a particular emphasis on the role of neutrophils and granulopoiesis.
Factors related to mental health effect among nursing students in Japan and the United States during the coronavirus pandemic: A cross‐sectional study
Authors: Kondo Akiko , Abuliezi Renaguli , Niitsu Kosuke , Naruse Kazuko , Oki Tomomi , Ota Erika , Ezeonwu Mabel C. 
Published: October 2022

AbstractIn the context of mental health, university students have been considered a vulnerable population. However, limited studies have underscored the association between preventive health behaviour levels and mental health effects among nursing students. The current cross‐sectional study provides a comparative analysis of the impact of mental health factors on nursing students in Japan and the United States (US) in the context of the coronavirus disease 2019 (COVID‐19) pandemic. The study consisted of 878 participants, comprising both undergraduate and graduate nursing students from four universities in Japan, and one from the US. Hierarchical logistic regression was used to analyse the participant data in this study. In contrast to the American students, the Japanese students demonstrated significantly lower levels of perceived control and significantly higher levels of preventive health behaviours. Furthermore, Japanese students exhibited significantly higher levels of stress and/or symptoms of depression induced by the social distancing orders compared to the American students (z = −4.218, P < 0.001). However, no difference was observed after adjusting for perceived control, individual factors, socio‐economic factors, and preventive behaviours. During the pandemic, risk factors that can worsen mental health among the nursing students included younger age [odds ratio (95%CI) = 0.62 (0.48–0.81)], women [OR = 2.17 (1.02–4.61)], higher preventive health behaviour [OR = 1.05 (1.02–1.08)], lower perceived control [OR = 0.97 (0.94–0.99)], and lower perceived health competence [OR = 0.93 (0.90–0.96)]. Thus, this study recommends establishing training programmes that enhance perceived control and perceived health competence while encouraging preventive behaviour to support the mental health of nursing students, particularly young female students.
Management of wounds with exposed bone structures using an induced‐membrane followed by polymethyl methacrylate and second‐stage skin grafting in the elderly with a 3‐year follow‐up
Authors: Cai Leyi , Hong Zipu , Zhang Yingying , Xiang Guangheng , Luo Peng , Gao Weiyang , Li Zhijie , Zhou Feiya 
Published: October 2022

AbstractThe treatment of traumatic wounds with exposed bone or tendons is often challenging. An induced membrane (IM) is used to reconstruct bone defects, as it provides an effective and sufficient blood supply for bone and soft‐tissue reconstruction. This study explored a novel two‐stage strategy for wound management, consisting of initial wound coverage with polymethyl methacrylate (PMMA) and an autologous split‐thickness skin graft under the IM. Fifty inpatients were enrolled from December 2016 to December 2019. Each patient underwent reconstruction according to a two‐stage process. In the first stage, the defect area was thoroughly debrided, and the freshly treated wound was then covered using PMMA cement. After 4‐6 weeks, during the second stage, the PMMA cement was removed to reveal an IM covering the exposed bone and tendon. An autologous split‐thickness skin graft was then performed. Haematoxylin and eosin (H&E) staining and immunohistochemical analysis of vascular endothelial growth factor (VEGF), CD31 and CD34 were used to evaluate the IM and compare it with the normal periosteal membrane (PM). The psychological status and the Lower Extremity Function Scale (LEFS) as well as any complications were recorded at follow‐up. We found that all skin grafts survived and evidenced no necrosis or infection. H&E staining revealed vascularised tissue in the IM, and immunohistochemistry showed a larger number of VEGF‐, CD31‐ and CD34‐positive cells in the IM than in the normal PM. The duration of healing in the group was 5.40 ± 1.32 months with a mean number of debridement procedures of 1.92 ± 0.60. There were two patients with reulceration in the group. The self‐rating anxiety scale scores ranged from 35 to 60 (mean 48.02 ± 8.12). Postoperatively, the LEFS score was 50.10 ± 9.77. Finally, our strategy for the management of a non‐healing wound in the lower extremities, consisting of an IM in combination with skin grafting, was effective, especially in cases in which bony structures were exposed in the elderly. The morbidity rate was low.
Systematic review of groin wound surgical site infection incidence after arterial intervention
Authors: Gwilym Brenig Llwyd , Locker Dafydd Tudor , Matthews Emily Kate , Mazumdar Eshan , Adamson Georgia , Wall Michael Lewis , Bosanquet David Charles 
Published: October 2022

AbstractThe objectives were to determine the surgical site infection incidence (including superficial/deep) fter arterial intervention through non‐infected groin incisions and identify variables associated with incidence. MEDLINE, EMBASE and CENTRAL databases were searched for randomised controlled trials and observational studies of adults undergoing arterial intervention through a groin incision and reported surgical site infection. Infection incidence was examined in subgroups, variables were subjected to meta‐regression. One hundred seventeen studies reporting 65 138 groin incisions in 42 347 patients were included. Overall surgical site infection incidence per incision was 8.1% (1730/21 431): 6.3% (804/12 786) were superficial and 1.9% (241/12 863) were deep. Superficial infection incidence was higher in randomised controlled trials (15.8% [278/1762]) compared with observational studies (4.8% [526/11 024]); deep infection incidence was similar (1.7% (30/1762) and 1.9% (211/11 101) respectively). Aneurysmal pathology (β = −10.229, P < .001) and retrospective observational design (β = −1.118, P = .002) were associated with lower infection incidence. Surgical site infection being a primary outcome was associated with a higher incidence of surgical site infections (β = 3.429, P = .017). The three‐fold higher incidence of superficial surgical site infection reported in randomised controlled trials may be because of a more robust clinical review of patients. These results should be considered when benchmarking practice and could inform future trial design.
Achieving diversity in study populations: The importance of community engagement
Authors: Brangman Sharon A. 
Published: October 2022

AbstractThe majority of clinical trials currently and historically do not include older adults or non‐white participants. While more women are being recruited, their numbers are still limited. It is very hard to interpret trial results and apply them to older adults when their participation in clinical trials is limited. The focus of this article is the lack of clinical trial participation by persons of diverse races and ethnicities and the presentation of a model infrastructure grounded in community engagement that is proving to be effective in increasing the interest and participation of older African Americans in research.
Neurochemical, histological, and behavioral profiling of the acute, sub‐acute, and chronic MPTP mouse model of Parkinsonʼs disease
Authors: Santoro Matteo , Fadda Paola , Klephan Katie J. , Hull Claire , Teismann Peter , Platt Bettina , Riedel Gernot 
Published: October 2022

AbstractParkinson's disease (PD) is a heterogeneous multi‐systemic disorder unique to humans characterized by motor and non‐motor symptoms. Preclinical experimental models of PD present limitations and inconsistent neurochemical, histological, and behavioral readouts. The 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) mouse model of PD is the most common in vivo screening platform for novel drug therapies; nonetheless, behavioral endpoints yielded amongst laboratories are often discordant and inconclusive. In this study, we characterized neurochemically, histologically, and behaviorally three different MPTP mouse models of PD to identify translational traits reminiscent of PD symptomatology. MPTP was intraperitoneally (i.p.) administered in three different regimens: (i) acute—four injections of 20 mg/kg of MPTP every 2 h; (ii) sub‐acute—one daily injection of 30 mg/kg of MPTP for 5 consecutive days; and (iii) chronic—one daily injection of 4 mg/kg of MPTP for 28 consecutive days. A series of behavioral tests were conducted to assess motor and non‐motor behavioral changes including anxiety, endurance, gait, motor deficits, cognitive impairment, circadian rhythm and food consumption. Impairments in balance and gait were confirmed in the chronic and acute models, respectively, with the latter showing significant correlation with lesion size. The sub‐acute model, by contrast, presented with generalized hyperactivity. Both, motor and non‐motor changes were identified in the acute and sub‐acute regime where habituation to a novel environment was significantly reduced. Moreover, we report increased water and food intake across all three models. Overall, the acute model displayed the most severe lesion size, while across the three models striatal dopamine content (DA) did not correlate with the behavioral performance. The present study demonstrates that detection of behavioral changes following MPTP exposure is challenging and does not correlate with the dopaminergic lesion extent.image
Dispossession through land titling: Legal loopholes and shadow procedures to urbanized forestlands in the Yucatán Peninsula
Authors: Torres‐Mazuera Gabriela 
Published: October 2022

AbstractUnder certain circumstances, land titling, property regime changes, and land‐use conversions yield substantial profits. Yet few people possess the wealth, knowledge, and networks to benefit from these procedures. In the Yucatán Peninsula, a region recently targeted as a prominent investment location by the Mexican national government (mainly with the “Tren Maya” megaproject) and the private capital, forestlands collectively owned as ejidos by Mayan peasants are on the trend to complete privatization. Against the arguments of neo‐institutional economists that in the 1990s promoted legal reforms and justified land‐titling programmes worldwide to make available credit for uncapitalized peasants, individual land titling of commonly held lands in Mexico increased the overall economic value of the land, but not the investment in economic activities related to farmland and indigenous communities. Instead, those policies enabled land grabbing, dispossession, and urbanization. In this article, I describe recent private‐led initiatives of ejido land titling that have redefined agricultural land's uses, meanings, and values for capitalist accumulation. In doing so, I explain how and why Mayan ejidatarios have been excluded from the monetary benefits of land titling, a top‐bottom dispossession process only accomplished through shadow procedures and former privatization of ejidos' common lands.
Adding fun or function: Considering new product formulations for market expansion with a focus on hangover solutions
Authors: Park Yeowoon , Lee Dongmin , Jeong Jaeseok , Moon Junghoon 
Published: October 2022

AbstractThis article aims to investigate the relationship between product formulations and consumers' willingness to buy (WTB), with the moderating role of the consumption situation. A 2 (utilitarian formulation vs. hedonic formulation) × 2 (unjustifiable situation vs. justifiable situation) between‐subjects design was constructed, and two specific formulations (pills and ice cream) were selected to represent a utilitarian formulation and hedonic formulation, respectively. Then, the hangover solution–consumption–situation was manipulated by varying drinking occasions. The results show that to strengthen the consumers' WTB a utilitarian product with such hedonic characteristics, consumers' justification process associated with hedonic consumption must be facilitated. This study is one of the first to address the notion that formulation differentiation can be a viable strategy in adding new features to a product.Practical ApplicationsConsidering that the global health supplement market is growing, this study hopes to provide practitioners with useful insights regarding new product development for functional products. The findings of this study indicate that if companies aim to strengthen the WTB a utilitarian product with such hedonic characteristics, the justification process associated with hedonic consumption must be facilitated. For example, to boost sales, marketers can target specific days, such as Fridays or Saturdays, when consumers can more easily justify the hedonic consumption associated with drinking (i.e., drinking behavior, choosing a hangover solution product).
A weighted blanket increases pre‐sleep salivary concentrations of melatonin in young, healthy adults
Authors: Meth Elisa M. S. , Brandão Luiz Eduardo Mateus , van Egmond Lieve T. , Xue Pei , Grip Anastasia , Wu Jiafei , Adan Ayaat , Andersson Filip , Pacheco André P. , Uvnäs‐Moberg Kerstin , Cedernaes Jonathan , Benedict Christian 
Published: October 2022

SummaryWeighted blankets have emerged as a potential non‐pharmacological intervention to ease conditions such as insomnia and anxiety. Despite a lack of experimental evidence, these alleged effects are frequently attributed to a reduced activity of the endogenous stress systems and an increased release of hormones such as oxytocin and melatonin. Thus, the aim of the present in‐laboratory crossover study (26 young and healthy participants, including 15 men and 11 women) was to investigate if using a weighted blanket (~12% of body weight) at bedtime resulted in higher salivary concentrations of melatonin and oxytocin compared with a light blanket (~2.4% of body weight). We also examined possible differences in salivary concentrations of the stress hormone cortisol, salivary alpha‐amylase activity (as an indicative metric of sympathetic nervous system activity), subjective sleepiness, and sleep duration. When using a weighted blanket, the 1 hour increase of salivary melatonin from baseline (i.e., 22:00) to lights off (i.e., 23:00) was about 32% higher (p = 0.011). No other significant differences were found between the blanket conditions, including subjective sleepiness and total sleep duration. Our study is the first to suggest that using a weighted blanket may result in a more significant release of melatonin at bedtime. Future studies should investigate whether the stimulatory effect on melatonin secretion is observed on a nightly basis when frequently using a weighted blanket over weeks to months. It remains to be determined whether the observed increase in melatonin may be therapeutically relevant for the previously described effects of the weighted blanket on insomnia and anxiety.
Permanent scars: The effects of wages on productivity
Authors: Fontanari Claudia , Palumbo Antonella 
Published: October 2022

AbstractThis paper explores, from a classical‐Keynesian theoretical standpoint, how stagnating real wages may have contributed to the slowdown of US productivity. Through shift‐share analysis, we find that after a sharp change in distribution against wages, some historically high‐productivity sectors switched towards slower productivity growth. This supports our hypothesis that the anemic growth of productivity may be partly due to the trend toward massive use of cheap labor. Our estimation of Sylos Labini's productivity equation confirms the existence of two direct effects of wages, one acting through the incentive to mechanization and the other through the incentive to reorganize labor use.
Design and performance evaluation of steel beam members with plate‐type locally resonant metamaterials for vibration control
Authors: Choi Jewoo , Cho Tongjun , Park Hyo Seon 
Published: October 2022

AbstractIn this study, a plate‐type locally resonant metamaterial (PTLRM) is proposed to control bending vibrations and low‐frequency noise of civil members. Considering the ease of design, installation, and workability, the PTLRM was fabricated using high‐density and soft materials. The PTLRM design includes a resonance frequency calculation of the unit and band gap estimation of the PTLRMs‐coupled structure. Three indicators were developed and experimentally analyzed to evaluate the vibration attenuation performance of the PTLRM: the response suppression in the resonance of PTLRMs, peak response suppression ratio, and distance between peaks. Experimental results of a 5‐m long steel beam with PTLRMs indicated that the bending modal responses of the steel beam were suppressed at frequencies closer to the local resonance of the PTLRM. This showed reasonable agreement with the results of the estimation. The acceleration of the PTLRMs‐coupled beam was reduced by up to 98.58% in the frequency response function. The time history of acceleration also decreased significantly over the entire time period. The damping of the soft material had little effect on the bandwidth of the band gap. However, it was found that a proper increase in the damping of the soft material could increase the vibration suppression performance.
Support nursing researchers' need from academic societies duringCOVID‐19: A cross‐sectional survey
Authors: Nagata Kyoko , Tanaka Koji , Takahashi Yutaro , Asada Yuya , Shimpuku Yoko , Yoshinaga Naoki , Sugama Junko 
Published: October 2022

AbstractThe activities of nursing researchers have been constrained by the COVID‐19 pandemic. Therefore, this study aimed to investigate the types of support and related factors that nursing researchers hope to receive from academic societies during the pandemic and to obtain suggestions for the role of academic societies in supporting nursing researchers and expanding research. An online survey was conducted with 1532 Japan Academy of Nursing Science members. The survey included 19 items of potential support from the society during the COVID‐19 pandemic as well as open‐ended questions. Data were analyzed statistically and qualitatively. For 9 of the 19 items, over 50% of respondents reported that they “needed” or “very much needed” support. Multivariate analysis results showed that younger respondents and those with family members requiring care were significantly more likely to report needs for some items. In the open‐ended comments, there were several suggestions for activities, including “Lobbying for revision of regulations on research implementation.” Nursing researchers expressed needs for support that reflect their demographic characteristics and situations, including collaboration across organizations and securing research‐promotion and skill‐development opportunities online.