bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Performance COD Detection of Organic Compounds Pollutants using Sulfurized-TiO2/Ti Nanotubes Arrays Photoelectrocatalyst
Authors: Nurdin Muhammad , Muzakkar Muhammad Zakir , Maulidiyah Maulidiyah , Trisna Trisna , Arham Zul , Salim La Ode Agus , Irwan Irwan , Umar Akrajas Ali 
Published: March 2022

Abstract
Photoelectrochemical oxidation processes offer a highly sensitive method to detect organic pollutants from wastewaters. Photoactive semiconducting material is the key factor in this process that accelerates the oxidation of the organic pollutant agent for sensitive detection. Chalcogenide doped TiO2 system has so far come up as a potential photocatalyst system for photoelectrochemical detection of organic compounds. Here, we report a highly sensitive detection of chemical oxygen demand of organic compound pollutant using sulfurized-TiO2 (S-TiO2/Ti) nanotube arrays (NTA) photoelectrocatalyst. S-TiO2/Ti NTA that is prepared via consecutive anodization of Ti sheet and TiO2 sulfurization process exhibited a high sensitivity to COD value of organic compounds pollutant, such asRed HE-3B (Reactive Red 120), Red HE-7B (Reactive Red 141), and Blue HE-RD (Reactive Blue 160). Our results indicated that the COD value is linearly correlated with the concentration of the organic compounds and is close to the COD theoretical value, inferring the high accuracy of the detection. This explains that the new S-TiO2/Ti NTA photoelectrocatalyst effectively enhances the electron transfer dynamic during the electrochemical measurement of an organic pollutant that is reflected by an impressive proportionality of electrochemical current to the concentration of the pollutant sample. The S-TiO2/Ti NTA promises a highly efficient pollutant detection in the environment.
The Importance of Urban Eco-gardens in Biodiversity and Human Sustainability: a Case Study From Palestine
Authors: Qumsiyeh Mazin B. , Najajrah Mohammad H. , Handal Elias N. , Gideon Johann , Abusarhan Mohammed A. , Prasse Ruediger 
Published: March 2022

Abstract
There has been increased interest in urban gardens and small botanical gardens as sites for conservation of plants especially in developing countries. This was highlighted by the fact that most countries failed to meet the CBD Aichi 2020 targets and will now restructure to address the post-2020 CBD framework. The situation in Palestine is of concern where global threats of climate change, overexploitation, habitat destruction, invasive species, and pollution are compounded by occupation and conflict. Thus, almost 1/3rd of vascular plant species are rare and over 50 are listed as endangered or threatened. Here we describe the development of a conservation botanic garden that works via research, education, and direct in situ and ex situ conservation of plant species. The garden now boasts 381 species of vascular plants (63 are rare). The team scientifically and selectively introduced some rare and endemic species, developed educational modules, and engaged the community in all aspects of planning and growth of this garden. It acted as a model for nearby threatened areas and for many of the protected areas in the state of Palestine. This has become a national oasis for both wildlife and humans. The lessons learned from this experience included: 1) principles of minimal intervention in eco-friendly ways producing zones of permaculture, gardening towards conservation (ex situ and in situ conservation), while allowing botanic garden functionaliuty, 2) involvement of staff, volunteers, experts, and community in education and conservation efforts, 3) value of research in plants and animals for integrated ecosystem management. The outcome of the work was a maximally utilitarian garden for areas like education, direct conservation, research, and aesthetics (people enjoyment) while ensuring sustainability long term in a nascent state under difficult political situation.
IRAK1 Duplication in MECP2 Duplication Syndrome Does Not Increase Canonical NF-κB-Induced Inflammation
Authors: Gottschalk Ilona , Kölsch Uwe , Wagner Dimitrios L. , Kath Jonas , Martini Stefania , Krüger Renate , Puel Anne , Casanova Jean-Laurent , Jezela-Stanek Aleksandra , Rossi Rainer , Chehadeh Salima El , Esch Hilde Van , von Bernuth Horst 
Published: March 2022

Abstract
Purpose Besides their developmental and neurological phenotype, most patients with MECP2/IRAK1 duplication syndrome present with recurrent and severe infections, accompanied by strong inflammation. Respiratory infections are the most common cause of death. Standardized pneumological diagnostics, targeted anti-infectious treatment and knowledge of the underlying pathomechanism that triggers strong inflammation are unmet clinical needs. We investigated the influence of IRAK1 overexpression on the canonical NF-κB signaling as a possible cause for excessive inflammation in these patients. Methods NF-κB signaling was examined by measuring the production of proinflammatory cytokines as well as evaluating the IRAK1 phosphorylation and degradation as well as the IκBα-degradation upon stimulation with IL-1β and TLR-agonists in SV40 immortalized fibroblasts, PBMCs and whole blood of 9 patients with MECP2/IRAK1 duplication syndrome, respectively. Results Both, MECP2/IRAK1-duplicated patients and healthy controls, showed similar production of IL-6 and IL-8 upon activation with IL-1β and TLR4/2/6-agonists in immortalized fibroblasts, PBMCs and whole blood. Patients and controls have equivalent patterns of IRAK1 phosphorylation and degradation as well as IκBα degradation upon stimulation with IL-1β. Conclusion Patients with MECP2/IRAK1 duplication syndrome do not show increased canonical NF-κB signaling in immortalized fibroblasts, PBMCs and whole blood. Therefore, we assume that these patients do not benefit from a therapeutic suppression of this pathway.
Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes
Authors: Terajima Masahiko , Taga Yuki , Nakamura Tomoyuki , Guo Hou-Fu , Kayashima Yukako , Maeda-Smithies Nobuyo , Parag-Sharma Kshitij , Amelio Antonio L. , Mizuno Kazunori , Kurie Jonathan M. , Yamauchi Mitsuo 
Published: March 2022

Abstract
Lysyl hydroxylase 2 (LH2) is a member of LH family that catalyzes the hydroxylation of lysine (Lys) residues on collagen, and this particular isozyme has been implicated in various diseases. While its function as a telopeptidyl LH is generally accepted, several fundamental questions remain unanswered: 1, Does LH2 catalyze the hydroxylation of all telopeptidyl Lys residues of collagen? 2, Is LH2 involved in the helical Lys hydroxylation? 3, what are the functional consequences when LH2 is completely absent? To answer these questions, we generated LH2-null MC3T3 cells (LH2KO), and extensively characterized the type I collagen phenotypes in comparison with controls. Cross-link analysis demonstrated that the hydroxylysine-aldehyde (Hylald)-derived cross-links were completely absent from LH2KO collagen with concomitant increases in the Lysald-derived cross-links. Mass spectrometric analysis revealed that, in LH2KO type I collagen, telopeptidyl Lys hydroxylation was completely abolished at all sites while helical Lys hydroxylation was slightly diminished in a site-specific manner. Moreover, di-glycosylated Hyl was diminished at the expense of mono-glycosylated Hyl. LH2KO collagen was highly soluble and digestible, fibril diameters were diminished, and mineralization impaired when compared to controls. Together, these data underscore the critical role of LH2-catalyzed collagen modifications in collagen stability, organization and mineralization.
Detection of Land Subsidence using Sentinel-1 interferometer and its relationship with Sea-Level-Rise, Groundwater, and Inundation: A case study along Mumbai Coastal city
Authors: Nalakurthi N V Sudha Rani Nalakurthi , Behera Manasa Ranjan 
Published: March 2022

Abstract
Mumbai, an amalgamation of seven Islands with distinct landforms, is being reclaimed for urban sprawl. It is India's financial capital, with a vast population and a large port for trade, owing to a high demand for working class. As one of the most densely inhabited cities in the world, it faces a significant seasonal water scarcity crisis, resulting in groundwater exploitation for domestic and industrial purposes. The two key consequences are land subsidence and the influence of sea levels on coastal communities. The current study looked at the rate of land subsidence and tried to figure out how groundwater exploitation affected it. The Sentinel-1 SAR imagery was gathered for this purpose, and during the months of October 2014 to May 2019. The findings show that land sinking is irregular and has no consistent pattern. However, the spatial pattern of land subsidence was more closely related to urban area than with other types of terrain. Between 2015 and 2019, the largest yearly subsidence rate observed with the Sentinel data was − 93 mm/yr where the mean is -28.8 mm/yr. Groundwater data from well observations showed a falling tendency over the same time period as well as over a longer time period. In addition, a study is being conducted to better understand sea level rise scenarios and their influence on coastal inundation in low-lying locations. According to the observed sea level scenarios, the upper regional sea level will rise 0.7 m, 0.9 m, and 1.06 m by 2055, 2085, and 2095, respectively. Whereas for RCP recent emission scenarios the highest SLR observed at 1.2 m. Furthermore, with an increase in 1 m SLR, nearly 1700 sq km of low-lying areas, or nearly 38 percent of total land, could be swamped by 2095 during a regular monsoon. Regrettably, if urban infrastructure continues to expand, current conditions will be undermined. Therefore, it is high time to address the land subsidence and its consequences for the solutions.
Sodium Butyrate Inhibits Osteogenesis In Human Periodontal Ligament Stem Cells By Suppressing Smad1 Expression: A Randomized Controlled Trial
Authors: Hou Jingyi , Xu Junji , Liu Yi , Zhang Haiping , Wang Sihan , Jiao Yao , Guo Lijia , Li Song 
Published: March 2022

Abstract
Background: Butyrate is a major subgingival microbial metabolite that is closely related to periodontal disease. It affects the proliferation and differentiation of mesenchymal stem cells. However, the mechanisms by which butyrate affects the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) remain unclear. Here, we investigated the effect of sodium butyrate (NaB) on the osteogenic differentiation of human PDLSCs. Methods: PDLSCs were isolated from human periodontal ligaments and treated with various concentrations of NaB in vitro. The cell counting kit-8 assay and flow cytometric analysis were used to assess cell viability. The osteogenic differentiation capabilities of PDLSCs were evaluated using the alkaline phosphatase activity assay, alizarin red staining, RT-PCR, western blotting and in vivo transplantation. Results: NaB decreased PDLSC proliferation and induced apoptosis in a dose- and time-depend manner. Additionally, 1 mM NaB reduced alkaline phosphatase activity, mineralization ability, and the expression of osteogenic differentiation-related genes and proteins. Treatment with a free fatty acids receptor 2 (FFAR2) antagonist and agonist indicated that NaB inhibited the osteogenic differentiation capacity of PDLSCs by affecting the expression of Smad1. Conclusion: Our findings suggest that NaB inhibits the osteogenic differentiation of PDLSCs by activating FFAR2 and decreasing the expression of Smad1.
Increased Brain Functional Connectivity Correlated With Neurocognitive Performance Decline in Patients With Left Frontal Glioma
Authors: Ueda Masaya , Usami Kiyohide , Yamao Yukihiro , Yamawaki Rie , Umaba Chinatsu , Liang Nan , Nankaku Manabu , Mineharu Yohei , Honda Masayuki , Hitomi Takefumi , Ikeguchi Ryosuke , Ikeda Akio , Miyamoto Susumu , Matsuda Shuichi , Arakawa Yoshiki 
Published: March 2022

Abstract
Objectives: The association between neurocognitive decline and changing functional connectivity in glioma patients remains unclear. This study aimed to investigate the correlation between brain oscillatory activity or functional connectivity and neurocognitive function in patients with left frontal glioma using resting-state electroencephalography (EEG).Methods: This was an observational study and conforms to all the STROBE guidelines. Participants comprised 18 consecutive patients diagnosed with newly left frontal glioma between April 2010 and March 2021 who underwent neuropsychological examination and electroencephalography. Forty seconds of artifact-free electroencephalographic data were selected, and current source density (CSD) or lagged phase synchronization (LPS) were analyzed using exact low-resolution electromagnetic tomography (eLORETA). We investigated associations of CSD and LPS with the Wechsler adult intelligence scale (WAIS), the Wechsler memory scale-revised (WMS-R), and the western aphasia battery aphasia quotient (WAB AQ) scores. Results: Among the 18 consecutive patients (mean age, 43 ± 13.15 years), the mean WAIS full-scale intelligence quotient (FSIQ), WMS-R general memory (GM), and WAB AQ scores were within standard value ranges, and none had aphasia. The correlation analysis showed lower GM score was associated with higher LPS value between the right anterior prefrontal cortex and the left superior parietal lobule in the beta1 band (13–20 Hz, R = -0.802, P = 0.012)Conclusion: Increases in functional connectivity between certain brain regions may potentially represent more common and early network changes in the brain that are associated with cognitive declines in patients with left frontal glioma.
Coming of Age: Renovation Premiums in Housing Markets
Authors: Mamre Mari Olsen , Sommervoll Dag Einar 
Published: March 2022

Abstract
We rely on novel textual analysis of real estate listings and identify renovated dwellings in a data set of Norwegian transactions to estimate the renovation premium in an urban housing market. The renovation premium is estimated by classical regression approaches as well as random forests models. The strength of the latter is that it allows for a more complex interplay between the renovation premium and explanatory variables. We find a significant positive renovation premium of 5-7 percent for renovated dwellings and a negative premium of 9-10 percent for unmaintained/neglected dwellings. These averages mask significant variation in these premiums over time. In particular, there is a counter-cyclical effect. In a hedonic price model, omitting renovation has implications for estimated short-term house price growth. We also find that unmaintained dwellings tend to transact more in the fourth quarter, indicating that parts of seasonal price variation reported in the literature are due to compositional variation with respect to renovation. This composition effect tends to bias price movement estimates downward, if uncontrolled for, as unmaintained dwellings tend to transact at a significantly lower price.
Clearance of senescent cells with ABT-263 improves biological functions of synovial mesenchymal stem cells from osteoarthritis patients
Authors: Endo Kentaro , Miura Yugo , Komori Keiichiro , Sekiya Ichiro 
Published: March 2022

Abstract
Background: Osteoarthritis (OA) is an age-related joint disease characterized by progressive cartilage loss. Synovial mesenchymal stem cells (MSCs) are anticipated as a cell source for OA treatment; however, synovial MSC preparations isolated from OA patients contain many senescent cells that inhibit cartilage regeneration through their senescence-associated secretory phenotype (SASP) and poor chondrogenic capacity. The aim of this study was to improve the biological function of OA synovial MSCs by removing senescent cells using the senolytic drug ABT-263. Methods: We pretreated synovial MSCs derived from 5 OA patients with ABT-263 for 24 h and then evaluated senescence-associated beta galactosidase (SA-β gal) activity, apoptosis, surface antigen expression, colony formation ability, and multipotency. Results: The ABT-263 pretreatment significantly decreased the percentage of SA-β gal–positive cells and induced early- and late-stage apoptosis. Cleaved caspase-3 was expressed in SA-β gal–positive cells. The pretreated MSCs formed greater numbers of colonies with larger diameters. The expression rate of CD34 was decreased in the pretreated cells. Differentiation assays revealed that ABT-263 pretreatment enhanced the adipogenic and chondrogenic capabilities of OA synovial MSCs. In chondrogenesis, the pretreated cells produced greater amounts of glycosaminoglycan and showed lower expression of senescence markers (p16 and p21) and SASP factors (MMP-13 and IL-6). Conclusion: Pretreatment of synovial MSCs from OA patients with ABT-263 can improve the function of the cells by selectively eliminating senescent cells. These findings indicate that ABT-263 could hold promise for the development of effective cell-based OA therapy.
Comparison of the Predictive Values of Different Lipid Parameters Combined with Carotid Intima-Media Thickness in Coronary Artery Disease
Authors: Dong Haojian , Hu Xiangming , Yu Bingyan , Li Wei , Zhou Langping , Lin Yan , Wang Weimian , Li Guang , Zhou Yingling 
Published: March 2022

Abstract
Background: Blood lipids disorder and atherosclerosis are closely related to coronary artery disease (CAD). This study aims to compare different blood lipid parameters combined with carotid intima-media thickness (cIMT) in predicting CAD.Methods: This was a retrospective study including patients who underwent coronary angiography for highly suspected CAD. Blood samples were taken for lipid profile analysis and cIMT was evaluated by carotid ultrasound. Logistic analysis was used to establish different models of different lipid parameters in predicting CAD. The area under the receiver operating characteristic curve (AUC) was used to examine the predictive value. The optimal lipid parameter was also used to explore the relationship with multi-vessel CAD. Results: Patients were classified into two groups based on whether CAD existed. There was no difference in low-density lipoprotein cholesterol (LDL-C) between the two groups. Compared with non-CAD patients, the CAD group had higher lipoprotein (a) (Lp (a)), apolipoprotein B/apolipoprotein A, total cholesterol/high-density lipoprotein cholesterol (HDL-C), triglyceride/HDL-C and LDL-C/HDL-C. According to the AUCs, Lp (a) combined with cIMT (AUC: 0.713, p < 0.001) had the best performance in predicting CAD compared to other lipid parameters. High levels of Lp (a) was also associated with multi-vessel CAD (odds ratio: 1.41, 95% confidence interval: 1.02–1.95, P = 0.036).Conclusion: For patients with highly suspected CAD, Lp (a) better improved the predictive value of CAD rather than most of blood lipid indices, especially in the absence of high levels of LDL-C. Lp (a) also can be used to predict the multi-vessel CAD.
A Novel Mcl-1 Inhibitor Synergizes with Venetoclax to Induce Apoptosis in Cancer Cells
Authors: Zhao Tianming , Zhan Huien , Xie Shurong , Jiang Cheng , Lin Shengbin , Tian Ling , Liu Fangshu , Zeng Hui , Chen Guo 
Published: March 2022

Abstract
BackgroundEvading apoptosis by overexpression of anti-apoptotic Bcl-2 family proteins is a hallmark of cancer cells and the Bcl-2 selective inhibitor venetoclax is widely used in treatment of hematologic malignancies. Mcl-1, another anti-apoptotic Bcl-2 family member, is recognized as the primary cause of resistance to venetoclax treatment. However, there is currently no Mcl-1 inhibitor approved for clinical use.MethodsPaired parental and Mcl-1 knockout H1299 cells were used to screen and identify a small molecule named MI-238, which could specifically kill Mcl-1 proficient cells. Molecular docking, fluorescence polarization (FP) and Isothermal titration calorimetry (ITC) were employed to study MI-238 binding to Mcl-1 protein. Immunoprecipitation (IP) and flow cytometry assay were performed to analyze the activation of pro-apoptotic protein Bak. Annexin V staining and western blot analysis of cleaved caspase 3 were employed to measure the cell apoptosis. Mouse xenograft AML model using luciferase-expressing Molm13 cells were employed to evaluate in vivo therapeutic efficacy. Bone marrow samples from newly diagnosed AML patients were collected to evaluate the therapeutic potency in clinical AML patients.ResultsHere, we show that MI-238, a novel and specific Mcl-1 inhibitor, can disrupt the association of Mcl-1 with BH3-only pro-apoptotic proteins, selectively leading to apoptosis in Mcl-1 proficient cells. Moreover, MI-238 treatment also potently induces apoptosis in acute myeloid leukemia (AML) cells. Notably, the combined treatment of MI-238 with venetoclax exhibited strong synergistic anti-cancer effects in AML cells in vitro, MOLM-13 xenografts mouse model and AML patient samples.ConclusionsThis study identified a novel and selective Mcl-1 inhibitor MI-238 and demonstrated that development of MI-238 provides a novel strategy to improve the outcome of venetoclax therapy in AML.
Oral Health Profile Status and Treatment Needs in the Salvadoran Elderly Population
Authors: Aguirre Guillermo Alfonso , Quezada Ruth Fernández , Escobar Wendy Yesenia , Aguirre Katleen Argentina , de Miguel Ángel Gil , Rivas Francisco José 
Published: March 2022

Abstract
It is characteristic of older adults to present physiological changes in oral tissues and increased dental loss, mainly as a consequence of periodontal disease and dental caries. Epidemiological surveillance contributes to the development of strategies aiming to improve the quality of life and life expectancy of this population group. Given the lack of epidemiological data, the purpose of this study was to determine the oral health profile status and treatment needs of the elderly population of El Salvador. To that end, we conducted secondary analysis of the results of a recent oral health survey of 471 Salvadorians aged 60 years and older. The variables under study were: socio-demographics, brushing frequency, oral hygiene was measured using the Simplified Oral Hygiene Index (OHI-S), Decayed, Missing, and Filled Teeth index (DMFT) diagnosed with International Caries Detection and Assessment System (ICDAS), periodontopathies diagnosed with Community periodontal index of treatment needs (CPITN), edentulism and treatment needs. Student’s t-test, χ2, the z-test and a confidence level p < 0.05 were used. Of the survey respondents, 42.68% presented deficient oral hygiene, while 52.02% brushed twice a day. The DMFT index score was determinated to be 23.38. The Salvadoran elderly had an average of 16 missing teeth, 6 decayed, 1 filled and 5 healthy; while one-third had total edentulism. The survey respondents had a low frequency of brushing, with most categorized as having “poor” or “very poor” oral hygiene. Almost all respondents had some degree of periodontal disease and required restorative intervention.