bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Photothermal-Response Oxygen Release Platform Based on Hydrogel for Accelerating Wound Healing
Authors: Jin Lin , Li Chunyang , He Xiaojun , Li Qingfeng , Lv Mingzhi , Shen Jianliang , He Deyan 
Published: July 2022

Abstract
During wound healing, oxygen availability and anti-inflammatory microenvironment play an important role in the formation of new tissue. However, whether to provide continuous and controllable oxygen around the injured tissue while inhibiting inflammation, and realize the synergistic effect of oxygen supply and anti-inflammation, is still a major problem affecting the regeneration and repair of wound tissue. Inspired by skin wound pathology and the inflammatory microenvironment, a photothermal response-assisted strategy has been developed in this work. We prepared composite hydrogel system of PDA-HA (polydopamine-hyaluronic acid) hydrogel-loaded CaO2-ICG@LA@MnO2 (calcium peroxide-indocyanine green @ lauric acid @ manganese dioxide) nanoparticles, which showed excellent photothermal performance under near-infrared (NIR) irradiation, and realized the on-off release of oxygen and reactive oxygen species (ROS). Controlled and sustained oxygen release can promote the regeneration and repair of damaged tissue, and the generated ROS can effectively inhibit the outbreak of inflammation at the initial stage of wound healing. We believe that the system we have obtained can be used as a new approach for treating chronic wound healing.
MRSA Panophthalmitis in a Brittle Diabetic: a Histopathologic Review
Authors: Flynn Erin , Lesche Stephen , Barak Stephanie , Ittoop Sabita , Mansour Tamer , Wroblewski Keith J. 
Published: July 2022

Abstract
A 30-year-old male presented with bilateral eye swelling and methicillin resistant staph aureous(MRSA) bacteremia from an outside hospital. His past medical history included poorly controlled type 1 diabetes mellitus, end stage renal disease on dialysis, heart failure with reduced ejection fraction, and bilateral below the knee amputations. With worsening bilateral periorbital swelling as well as concern for intracranial involvement/spread and for scleral melt on the left eye, the patient was taken for left eye enucleation with wound drainage/antibiotic irrigation. The entire globe was represented in the slide and it shows extensive intra-ocular, scleral and episcleral acute inflammation with multifocal abscess formation. MRSA orbital cellulitis is an aggressive disease and generally caused by external trauma or injury that seeds the gram-positive organism posterior to the orbital septum, although it can also be caused endogenously through systemic infection. Bilateral blindness from community-associated MRSA orbital cellulitis has also been seen in the literature. We present a histopathologic review of a case of MRSA panophthalmitis.
The effect of ZnO nanoparticles functionalized with glutamine and conjugated with thiosemicarbazide on triggering of apoptosis adenocarcinoma gastric (AGS) cell line
Authors: Beigi Sadaf , Salehzadeh Ali , Habibollahi Hadi , Shandiz Seyed Ataollah Sadat , Safa Fariba 
Published: July 2022

Abstract
Purpose
Gastric carcinoma is the fourth most common malignancy worldwide and remains the second cause of death of all malignancies worldwide. Today, despite advances in treatment, cancer is still the second leading cause of death worldwide. In the meantime, nanotechnology has helped to achieve fast and effective treatment. Today, Thiosemicarbazones has emerged as a new wave of drugs in recent years. Since these compounds alone have not been very successful in treating cancer, combining them with different nanoparticles has significantly increased the chances of success.
Methods
In this study, first Zinc Oxide (ZnO) nanoparticles were synthesized and then functionalized by glutamine (Gln) and finally conjugated to Thiosemicarbazide (ZnO@Gln-TSC) by co-precipitation method. Physicochemical method including FTIR, DLS, XRD, EDS-map, Zeta-potential, field emission scanning electron microscopy (FESEM) and imaging electron microscopy (TEM) techniques were used to investigate the synthesis of ZnO@Gln-TSC nanoparticles. The toxicity effects of ZnO@Gln-TSC nanoparticles and Oxaliplatin drug at different concentrations were investigated by MTT assay on adenocarcinoma gastric (AGS) cell line and normal cell line (HEK293). Apoptosis (programmed cell death) was also evaluated by Flow cytometry (Annexin V/ PI kit), cell cycle analysis and Hoechst staining.
Results
The synthesis of ZnO@Gln-TSC nanoparticles (NPs) was confirmed by chemical confirmation tests. The size of synthesized NPs was reported in the range of 40 to 70 nm. Also, a number of -31.7 mV in the Zeta-potential test indicated the stability of the produced nanoparticle. EDS analysis showed the elements of final NPs. DLS analysis showed the size of the hydrated NP at 374 nm. MTT test showed that the IC50 of NPs was 9.8 µg/ml and Oxaliplatin drug 65.7 µg/ml for cancer cells in comparison the IC50 of 150 µg/ml for normal cells. Hoechst staining confirmed that the NPs can induce apoptosis and cell death in cancer cell line. Flow cytometry analysis showed apoptosis induction among NPs treated cells than controls with the frequency of 55.5% and 9.48% for the early and late apoptosis, respectively.
Conclusion
This study revealed the promising anticancer potential of NPs to be used for gastric cancer treatment after further characterization using in-vitro and in-vivo assays.
Mahonia alkaloids (MA) ameliorate depression induced gap junction dysfunction by miR-205/Cx43 axis
Authors: He Junhui , Li Dongmei , Wei Jie , Wang Sheng , Chu Shifeng , Zhang Zhao , He Fei , Wei Dongmei , Li Yi , Xie Jiaxiu , Wei Guining , Chen Naihong 
Published: July 2022

Abstract
Depression has become an important disease threatening human health. In recent years, the efficacy of Traditional Chinese Medicine (TCM) in treating the disease has become increasingly prominent, so it is meaningful to find new antidepressant TCM. Mahonia fortune (Lindl.) Fedde is a primary drug in traditional formulas for the treatment of depression, however, the exact mechanism of its action is unclear. This study aimed to research the effect of MA on the improvement of gap junction function in depression via the miR-205/Cx43 pathway. The antidepressant effects of MA were observed by a rat model of reserpine-induced depression and a model of corticosterone (CORT)-induced astrocytes. The concentrations of neurotransmitters were measured by ELISA, the expression of Connexin 43 (Cx43) protein was measured by Immunohistochemistry, the expression of Cx43, BDNF, CREB proteins were measured by western-blot, the pathological changes of prefrontal cortex were observed by hematoxylin-eosin (H&E) staining. The binding of miR-205 and Cx43 was verified with a Luciferase reporter gene. Gap junction dysfunction detected by fluorescent yellow staining. The results confirmed that MA remarkably decreased miR-205 expression and increased Cx43, BDNF, CREB expression in depression rat and CORT-induced astrocytes. In addition, after overexpression of miR-205 in vitro, MA significantly increased Cx43, BDNF and CREB expression in CORT-induced astrocytes. Thus, MA may target Cx43 through miR-205 to regulate CREB/BDNF signaling pathway to improve depressive behavior.
Discovery and Significance of Green and Selenium-Rich of Upland Rice in Xinfeng County, Jiangxi Province,China
Authors: WANG Yun , Lan yang , zou Yong-jun , qiu su-yan 
Published: July 2022

Abstract
Elemental selenium deficiency is considered as a global problem about the health of human that can be alleviated by daily selenium supplementation from selenium-rich foods. In order to discover some green selenium-enriched agricultural products, several samples including surface soil, upland rice and root soil collected from the camellia interplanting upland rice in Xinfeng County, Jiangxi Province were tested, analyzed for selenium and heavy metals elements, and evaluated their quality. The results show that the surface soil is strongly acidic in this area, the average selenium content grade is moderate, and the soil environmental geochemistry grade is clean. For the upland rice, it is found that the root-soil system is strong acid, the average selenium content grade is selenium-rich, and the soil environmental geochemistry grade is clean. Additionally, the vertical profile of the soil reveals that Cd, Hg, Cr, and organic matter exhibit an obvious enrichment trend on the surface, and the content of Se is positively correlated with the content of Cd, As, Hg, Cr, and organic matter respectively, while is negative with the content of Ni, Cu, Zn, Pb and pH respectively, which indicates that the upland rice is at risk of heavy metal pollution. Nervertheless, the upland rice grown in this area is green and selenium-rich, which suggests a good ability to repair the soil contaminated by Hg and Cd heavy metals through the upland rice.
Extremely reduced supergroup F Wolbachia: transition to obligate insect symbionts
Authors: Mahmood Sazzad , Nováková Eva , Martinů Jana , Sychra Oldřich , Hypša Václav 
Published: July 2022

Abstract
Background
Wolbachia belong to highly abundant bacteria which are frequently found in invertebrate microbiomes and manifest by a broad spectrum of lifestyles from parasitism to mutualism. Wolbachia Supergroup F is a particularly interesting group as it gave rise to symbionts of both arthropods and nematodes, and some of its members are obligate mutualists. Investigations on evolutionary transitions among the different symbiotic stages have been hampered by a lack of the known diversity and genomic data for the supergroup F members.
Results
Based on amplicon screening, short and long read WGS approaches, and laser confocal microscopy, we characterize five new supergroup F Wolbachia strains from four chewing lice species. These strains reached different evolutionary stages and represent two remarkably different types of symbiont genomes. Three of the genomes resemble other known members of Wolbachia F supergroup, while other two show typical signs of ongoing gene inactivation and removal (genome size, coding density, low number of pseudogenes). Particularly, wMeur1, a symbiont fixed in microbiomes of Menacanthus eurysternus across four continents, possesses a highly reduced genome of 733,850 bp. The horizontally acquired capacity for pantothenate synthesis and localization in specialized bacteriocytes suggest its obligate nutritional role.
Conclusions
The genome of wMeur1 strain, from the M. eurysternus micobiome, represents the smallest currently known Wolbachia genome and the first example of Wolbachia which has completed genomic streamlining as known from the gammaproteobacterial obligate symbionts. This points out that despite the large amount and great diversity of the known Wolbachia strains, evolutionary potential of these bacteria still remains underexplored. The diversity of the four chewing lice microbiomes indicates that this vast parasitic group may provide suitable models for further investigations.
Complete mitochondrial genomes and updated divergence time of the two freshwater clupeids endemic to Lake Tanganyika (Africa) suggest intralacustrine speciation
Authors: Milec Leona J.M. , Vanhove Maarten P.M. , Bukinga Fidel Muterezi , Keyzer Els L.R. , Kapepula Vercus Lumami , Mulungula Pascal Masilya , Mulimbwa N’Sibula , Wagner Catherine E. , Raeymaekers Joost A.M. 
Published: July 2022

Abstract
BackgroundThe hydro-geological history of Lake Tanganyika paints a complex image of several colonization and adaptive radiation events. The initial basin was formed around 9-12 million years ago (MYA) from the predecessor of the Malagarasi–Congo River and only 5-6 MYA, its sub-basins fused to produce the clear, deep waters of today. Next to the well-known radiations of cichlid fishes, the lake also harbours a modest clade of only two clupeid species, Stolothrissa tanganicae and Limnothrissa miodon. They are members of Pellonulini, a tribe of clupeid fishes that mostly occur in freshwater and that colonized West- and Central-Africa during a period of high sea levels during the Cenozoic. There is no consensus on the phylogenetic relationships between members of Pellonulini and the timing of the colonization of Lake Tanganyika by clupeids. ResultsWe use short-read next generation sequencing of 10X Chromium libraries to sequence and assemble the full mitochondrial genomes of S. tanganicae and L. miodon. We then use Maximum Likelihood and Bayesian methods to place them into the phylogeny of Pellonulini and other clupeiforms, taking advantage of all available full mitochondrial clupeiform genomes. We identify Potamothrissa obtusirostris as the closest living relative of the Tanganyika sardines and establish paraphyly for Microthrissa. Our results indicate a relatively recent divergence of the Tanganyika sardines around 4.07 MYA [95% CI: 1.66-7.06], and from P. obtusirostris around 11.88 MYA [95% CI: 6.09-17.27]. ConclusionsThese young estimates imply that the ancestor of the Tanganyika sardines diverged from a riverine ancestor and entered the proto-lake Tanganyika around the time of its formation from the Malagarasi–Congo river, and diverged into the two extant species at the onset of deep clearwater conditions. Our results prompt a more thorough examination of the relationships within Pellonulini, and the new mitochondrial genomes provide an important resource for the future study of this tribe, e.g. as a reference for species identification, genetic diversity, and macroevolutionary studies.
Translanguaging Pedagogy in Tutor’s Oral Corrective Feedback on Chinese EFL Learners’ Argumentative Writing
Authors: Wang Yibei , Li Danli 
Published: July 2022

Abstract
Translanguaging pedagogy has widely attracted interests from studies of second or foreign language teaching. Corrective feedback has long been recognized for its value on improving second language learning outcomes. However, limited attention has been given to the potential influence of translanguaging in the process of corrective feedback on learners’ second language writing performance, especially the influence of oral corrective feedback. This paper aims at exploring the effects of translanguaging in tutors’ oral corrective feedback (OCF) on Chinese EFL learners’ argumentative writing in six-week writing tutorials. 12 first-year Chinese college students and a Chinese tutor of English participated in the study at a university in Central China. The findings showed positive effects of translanguaging in OCF on Chinese EFL students’ argumentative writing performance. It attempts to make some contributions to EFL writing instruction. Teachers can alter their monolingual medium of English only to adapt to students’ needs, focus on students’ language practices, and create a language education ecological environment through translanguaging pedagogy.
Predictable Timing Behavior of Gracefully Degrading Automotive Systems
Authors: Weiss Philipp , Steinhorst Sebastian 
Published: July 2022

Abstract
Fail-operational behavior of safety-critical software for autonomous driving is essential as there is no driver available as a backup solution.In a failure scenario, safety-critical tasks can be restarted on other available hardware resources.Here, graceful degradation can be used as a cost-efficient solution where hardware resources are redistributed from non-critical to safety-critical tasks at run-time.We allow non-critical tasks to actively use resources that are reserved as a backup for critical tasks, which would be otherwise unused and which are only required in a failure scenario.However, in such a scenario, it is of paramount importance to achieve a predictable timing behavior of safety-critical applications to allow a safe operation. Here, it has to be ensured that even after the restart of safety-critical tasks a guarantee on execution times can be given.
In this paper, we propose a graceful degradation approach using composable scheduling.We use our approach to present, for the first time, a performance analysis which is able to analyze timing constraints of fail-operational distributed applications using graceful degradation.Our method can verify that even during a critical ECU failure, there is always a backup solution available which adheres to end-to-end timing constraints.Furthermore, we present a dynamic decentralized mapping procedure which performs constraint solving at run-time using our analytical approach combined with a backtracking algorithm.
We evaluate our approach by comparing mapping success rates to state-of-the-art approaches such as active redundancy and an approach based on resource availability. In our experimental setup our graceful degradation approach can fit about double the number of critical applications on the same architecture compared to an active redundancy approach.
Combined, our approaches enable, for the first time, a dynamic and fail-operational behavior of gracefully degrading automotive systems with cost-efficient backup solutions for safety-critical applications.
Predictive values of the modified Mallampati test, upper lip bite test, thyromental distance and ratio of height to thyromental distance to predict difficult laryngoscopy in pediatric elective surgical patients 5-12 years old at selected Addis Ababa governmental hospitals, Ethiopia: A multicenter cross-sectional study.
Authors: Sitot Mulualem , Amare Wubayehu , Aregawi Adugna 
Published: July 2022

Abstract
Background: Maintaining patent airways is vital in pediatric anesthetic management. Failure to manage and anticipate difficult laryngoscopy preoperatively is the leading cause of morbidity and mortality. Data on the predictive values of screening parameters in predicting difficult laryngoscopy (DL) are limited in children. Therefore, this study aimed to assess the predictive value of the modified Mallampati test (MMT), upper lip bite test (ULBT), thyromental distance (TMD), and ratio of height to thyromental distance (RHTMD) in predicting DL in children aged 5-12 years at selected Addis Ababa governmental hospitals in Ethiopia.Methods: A multicenter cross-sectional study was conducted on 141 elective pediatric surgical patients aged 5 to 12 years selected using a systematic random sampling technique from December 1, 2021, to April 30, 2022. Data were collected using structured questionnaires. The collected data were entered and analysed by SPSS version 26. Fisher’s exact test and receiver operating characteristic curve analysis were used to compare the accuracy of MMC, ULBT, TMD, and RHTMD against difficult laryngoscopy. A P value <0.05 was considered statistically significant.Results: The magnitude of difficult laryngoscopy was 15.6%. MMT has the highest sensitivity (86.4%), specificity (91.6%), and negative predictive value (NPV) (97.3%) compared to other tests. The ULBT also has a high sensitivity (72.7%), specificity (84%), and NPV (94.3%) with comparable diagnostic accuracy (90.8%) with the MMT (P<0.05). The sensitivity, specificity, positive predictive value (PPV), NPV, and accuracy of TMD were 63.6%, 95.8%, 73.7%, 93.4%, and 82.2%, respectively. The RHTMD has the lowest specificity (63.6%), PPV (22.5%), NPV (91.4%), and accuracy (56.7%) in predicting difficult laryngoscopy.Conclusion: The MMT and ULBT are good screening tests, followed by the TMD in predicting DL, while the RHTMD was the least accurate predictor. Because no single test has 100% predictive value, a combination of screening tests is advised in pediatrics for predicting difficult laryngoscopy.
Adenylate cyclase A amplification and functional diversification during Polyspondylium pallidum development
Authors: Kawabe Yoshinori , Schaap Pauline 
Published: July 2022

Abstract
Background: In Dictyostelium discoideum (Ddis) adenylate cyclase A (ACA) critically generates the cAMP oscillations that coordinate aggregation and morphogenesis. Unlike group 4 species like Ddis, other groups do not use cAMP to aggregate. However, deletion of cAMP receptors (cARs) or extracellular phosphodiesterase (PdsA) in Polyspondylium pallidum (Ppal, group 2) blocks fruiting body formation, suggesting that cAMP oscillations ancestrally control post-aggregative morphogenesis. In group 2, the acaA gene underwent several duplications. We deleted the three Ppal aca genes to identify roles for either gene and tested whether Ppal shows transient cAMP-induced cAMP accumulation, which underpins oscillatory cAMP signalling. Results: Unlike Ddis, pre-aggregative Ppal cells did not produce a pulse of cAMP upon stimulation with the cAR agonist 2'H-cAMP but acquired this ability after aggregation. Deletion of Ppal aca1, aca2 and aca3 yielded different phenotypes. aca1ˉ cells showed relatively thin stalks, aca2ˉ showed delayed secondary sorogen formation and aca3ˉ formed less aggregation centers. The aca1ˉaca2ˉ and aca1ˉaca3ˉ mutants combined individual defects, while aca2ˉaca3ˉ and aca1ˉaca3ˉaca2ˉ additionally showed >24 h delay in aggregation, with only few aggregates with fragmenting streams being formed. The fragments developed into small fruiting bodies with stalk and spore cells. Aggregation was restored in aca2ˉaca3ˉ and aca1ˉaca3ˉaca2ˉ by 2.5 mM 8Br-cAMP, a membrane-permeant activator of cAMP-dependent protein kinase (PKA). Like Ddis, Ppal sorogens also express the adenylate cyclases ACR and ACG. We found that prior to aggregation, Ddis acaˉ/ACG cells produced a pulse of cAMP upon stimulation with 2'H-cAMP, indicating that cAMP oscillations may not be dependent on ACA alone.Conclusions: The three Ppal replicates of acaA perform different roles in stalk morphogenesis, secondary branch formation and aggregation in Ppal, but act together to enable development by activating PKA. While even an aca1ˉaca3ˉaca2ˉ mutant still forms (some) fruiting bodies, suggesting little need for ACA-induced cAMP oscillations in this process, we found that ACG also mediated transient cAMP-induced cAMP accumulation. It therefore remains likely that post-aggregative Ppal morphogenesis is organized by cAMP oscillations, favouring a previously proposed model where cAR-regulated cAMP hydrolysis rather its synthesis dominates oscillatory behaviour.