bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioactivity of crude fucoidan extracted from Sargassum ilicifolium (Turner) C. Agardh
Authors: Tsou Min-Hsuan , Lee Cheng-Chang , Wu Zhi-Yuan , Lee Zui-Harng , Lin Hsiu-Mei 
Published: September 2022

AbstractFucoidan derived from brown algae has been shown to exhibit antitumor and antioxidant effects, so research on sulfated polysaccharides is increasing. The purpose of this study was to evaluate the characteristics and biological activity of fucoidan that was extracted at two temperatures (65 and 80 °C) from Sargassum ilicifolium (Turner) C. Agardh from five regions of Taiwan. The data show that there are significant differences in the yield, sulfate and total sugar content of Sargassum ilicifolium (Turner) C. Agardh grown in different locations in the same sea area. HPLC was used to determine the monosaccharide compositions of the fucoidan, which contains fucose, mannose, mannose, glucose and galactose and have a low molecular weight of less than 5 kDa, and then we will select the algae collected in Fugang, Taitung, for further biological activity research. The sampled Sargassum ilicifolium (Turner) C. Agardh at all five locations has a good polyphenol content, and it shows great DPPH radical scavenging activity, ABTS radical scavenging activity, Ferrous ion-chelating activity and Reducing power. The Sargassum ilicifolium (Turner) C. Agardh that was collected from Taitung Fugang is not toxic to L929 normal cells, but for A549 cancer cells and HCT116 cancer cells, it is known from the results that it has good cytotoxicity for A549 cancer cells. Thus, this study found that the Sargassum ilicifolium (Turner) C. Agardh that was collected from Taitung Fugang has significant antioxidant and anticancer properties.
Inferring the age of breeders from easily measurable variables
Authors: Genovart Meritxell , Klementisová Katarina , Oro Daniel , Fernández-López Pol , Bertolero Albert , Bartumeus Frederic 
Published: September 2022

AbstractAge drives differences in fitness components typically due to lower performances of younger and senescent individuals, and changes in breeding age structure influence population dynamics and persistence. However, determining age and age structure is challenging in most species, where distinctive age features are lacking and available methods require substantial efforts or invasive procedures. Here we explore the potential to assess the age of breeders, or at least to identify young and senescent individuals, by measuring some breeding parameters partially driven by age (e.g. egg volume in birds). Taking advantage of a long-term population monitored seabird, we first assessed whether age influenced egg volume, and identified other factors driving this trait by using general linear models. Secondly, we developed and evaluated a machine learning algorithm to assess the age of breeders using measurable variables. We confirmed that both younger and older individuals performed worse (less and smaller eggs) than middle-aged individuals. Our ensemble training algorithm was only able to distinguish young individuals, but not senescent breeders. We propose to test the combined use of field monitoring, classic regression analysis and machine learning methods in other wild populations were measurable breeding parameters are partially driven by age, as a possible tool for assessing age structure in the wild.
Plasma metabolome study reveals metabolic changes induced by pharmacological castration and testosterone supplementation in healthy young men
Authors: de Siqueira Guedes Jéssica , Pla Indira , Sahlin K. Barbara , Monnerat Gustavo , Appelqvist Roger , Marko-Varga György , Giwercman Aleksander , Domont Gilberto Barbosa , Sanchez Aniel , Nogueira Fábio César Sousa , Malm Johan 
Published: September 2022

AbstractTestosterone is a hormone that plays a key role in carbohydrate, fat, and protein metabolism. Testosterone deficiency is associated with multiple comorbidities, e.g., metabolic syndrome and type 2 diabetes. Despite its importance in many metabolic pathways, the mechanisms by which it controls metabolism are not fully understood. The present study investigated the short-term metabolic changes of pharmacologically induced castration and, subsequently, testosterone supplementation in healthy young males. Thirty subjects were submitted to testosterone depletion (TD) followed by testosterone supplementation (TS). Plasma samples were collected three times corresponding to basal, low, and restored testosterone levels. An untargeted metabolomics study was performed by liquid chromatography–high resolution mass spectrometry (UHPLC–HRMS) to monitor the metabolic changes induced by the altered hormone levels. Our results demonstrated that TD was associated with major metabolic changes partially restored by TS. Carnitine and amino acid metabolism were the metabolic pathways most impacted by variations in testosterone. Furthermore, our results also indicated that LH and FSH might strongly alter the plasma levels of indoles and lipids, especially glycerophospholipids and sphingolipids. Our results demonstrated major metabolic changes induced by low testosterone that may be important for understanding the mechanisms behind the association of testosterone deficiency and its comorbidities.
Femtosecond tunable solitons up to 4.8 µm using soliton self-frequency shift in an InF3 fiber
Authors: Gauthier Jean-Christophe , Olivier Michel , Paradis Pascal , Dumas Marie-Frédérique , Bernier Martin , Vallée Réal 
Published: September 2022

AbstractA tunable ultrashort soliton pulse source reaching up to 4.8 µm is demonstrated based on a 2.8 µm femtosecond fiber laser coupled to a zirconium fluoride fiber amplifier followed by a small core indium fluoride fiber. This demonstration is extending by 300 nm the long wavelength limit previously reported with soliton self-frequency shift (SSFS) sources based on fluoride fibers. Our experimental and numerical investigation highlighted the spectral dynamics associated with the generation of highly redshifted pulses in the mid-infrared using SSFS enhanced by soliton fission. This study is intended at providing a better understanding of the potential and limitations of SSFS based tunable femtosecond fiber sources in the 3–5 µm spectral range.
STAR sleep recording export software for automatic export and anonymization of sleep studies
Authors: Nikkonen Sami , Korkalainen Henri , Töyräs Juha , Leppänen Timo 
Published: September 2022

AbstractSleep research often relies on large retrospective clinical datasets. However, as the data is usually stored in proprietary formats specific for each sleep software, the raw data cannot be easily accessed and analyzed with external tools. While the raw data can usually be exported to more common data formats, this is often a cumbersome and labor-intensive task as it is not required for clinical purposes. Additionally, the recordings often include sensitive patient information which must be removed before the data can be shared or analyzed externally. This anonymization can be difficult to perform manually without the correct tools or knowledge of the file types and how they internally store the data. The STAR sleep recording export software provides a simple tool that can be used to perform the sleep study exports automatically. This allows the user to easily export a batch of sleep studies with minimal effort. In addition, the software can also be used to automatically anonymize the exported sleep recordings allowing researchers to save time and personnel resources as these do not need to be allocated for exporting and anonymizing sleep studies. The software supports Noxturnal, RemLogic, Profusion PSG and Sleepware G3 and it is free and openly available for anyone to download and use.
Surface potential modulation as a tool for mitigating challenges in SERS-based microneedle sensors
Authors: Brasiliense Vitor , Park Ji Eun , Berns Eric J. , Van Duyne Richard P. , Mrksich Milan 
Published: September 2022

AbstractRaman spectroscopic-based biosensing strategies are often complicated by low signal and the presence of multiple chemical species. While surface-enhanced Raman spectroscopy (SERS) nanostructured platforms are able to deliver high quality signals by focusing the electromagnetic field into a tight plasmonic hot-spot, it is not a generally applicable strategy as it often depends on the specific adsorption of the analyte of interest onto the SERS platform. This paper describes a strategy to address this challenge by using surface potential as a physical binding agent in the context of microneedle sensors. We show that the potential-dependent adsorption of different chemical species allows scrutinization of the contributions of different chemical species to the final spectrum, and that the ability to cyclically adsorb and desorb molecules from the surface enables efficient application of multivariate analysis methods. We demonstrate how the strategy can be used to mitigate potentially confounding phenomena, such as surface reactions, competitive adsorption and the presence of molecules with similar structures. In addition, this decomposition helps evaluate criteria to maximize the signal of one molecule with respect to others, offering new opportunities to enhance the measurement of analytes in the presence of interferants.
Pervasive exposure of wild small mammals to legacy and currently used pesticide mixtures in arable landscapes
Authors: Fritsch Clémentine , Appenzeller Brice , Burkart Louisiane , Coeurdassier Michael , Scheifler Renaud , Raoul Francis , Driget Vincent , Powolny Thibaut , Gagnaison Candice , Rieffel Dominique , Afonso Eve , Goydadin Anne-Claude , Hardy Emilie M. , Palazzi Paul , Schaeffer Charline , Gaba Sabrina , Bretagnolle Vincent , Bertrand Colette , Pelosi Céline 
Published: September 2022

AbstractKnowledge gaps regarding the potential role of pesticides in the loss of agricultural biodiversity worldwide and mixture-related issues hamper proper risk assessment of unintentional impacts of pesticides, rendering essential the monitoring of wildlife exposure to these compounds. Free-ranging mammal exposure to legacy (Banned and Restricted: BRPs) and currently used (CUPs) pesticides was investigated, testing the hypotheses of: (1) a background bioaccumulation for BRPs whereas a “hot-spot” pattern for CUPs, (2) different contamination profiles between carnivores and granivores/omnivores, and (3) the role of non-treated areas as refuges towards exposure to CUPs. Apodemus mice (omnivore) and Crocidura shrews (insectivore) were sampled over two French agricultural landscapes (n = 93). The concentrations of 140 parent chemicals and metabolites were screened in hair samples. A total of 112 compounds were detected, showing small mammal exposure to fungicides, herbicides and insecticides with 32 to 65 residues detected per individual (13–26 BRPs and 18–41 CUPs). Detection frequencies exceeded 75% of individuals for 13 BRPs and 25 CUPs. Concentrations above 10 ng/g were quantified for 7 BRPs and 29 CUPs (in 46% and 72% of individuals, respectively), and above 100 ng/g for 10 CUPs (in 22% of individuals). Contamination (number of compounds or concentrations) was overall higher in shrews than rodents and higher in animals captured in hedgerows and cereal crops than in grasslands, but did not differ significantly between conventional and organic farming. A general, ubiquitous contamination by legacy and current pesticides was shown, raising issues about exposure pathways and impacts on ecosystems. We propose a concept referred to as “biowidening”, depicting an increase of compound diversity at higher trophic levels. This work suggests that wildlife exposure to pesticide mixtures is a rule rather than an exception, highlighting the need for consideration of the exposome concept and questioning appropriateness of current risk assessment and mitigation processes.
Conformational transition induced in the aspartate:alanine antiporter by l-Ala binding
Authors: Suzuki Satomi , Chiba Fumika , Kimura Takuya , Kon Nanase , Nanatani Kei , Abe Keietsu 
Published: September 2022

AbstractAn aspartate:alanine antiporter (AspT) from the lactic acid bacterium Tetragenococcus halophilus catalyzes the electrogenic aspartate1-:alanine0 exchange reaction. Our previous kinetic analyses of transport reactions mediated by AspT in reconstituted liposomes suggested that, although the substrate transport reactions are physiologically coupled, the putative binding sites of l-aspartate (-Asp) and l-alanine (-Ala) are independently located on AspT. By using the fluorescent probe Oregon Green maleimide (OGM), which reacts specifically with cysteine, we also found that the presence of l-Asp changes the conformation of AspT. In this study, we conducted an OGM labeling assay in the presence of l-Ala. The labeling efficiency of single cysteine mutants (G62C and P79C) in transmembrane helix 3 of the AspT showed novel patterns depending on the presence of l-Ala or analogs. A concentration-dependent shift of AspT from the conformation in the presence of one substrate to that specific to the substrate added subsequently (l-Ala or l-Asp) was observed. Moreover, size-exclusion-chromatography-based thermostability assays indicated that the thermal stability of AspT in the presence of l-Ala differed from that in the presence of l-Asp. From these results, we concluded that l-Ala binding yields a conformation different from the apo or l-Asp binding conformations.
Nondestructive protein sampling with electroporation facilitates profiling of spatial differential protein expression in breast tumors in vivo
Authors: Vitkin Edward , Singh Amrita , Wise Julia , Ben-Elazar Shay , Yakhini Zohar , Golberg Alexander 
Published: September 2022

AbstractExcision tissue biopsy, while central to cancer treatment and precision medicine, presents risks to the patient and does not provide a sufficiently broad and faithful representation of the heterogeneity of solid tumors. Here we introduce e-biopsy—a novel concept for molecular profiling of solid tumors using molecular sampling with electroporation. As e-biopsy provides access to the molecular composition of a solid tumor by permeabilization of the cell membrane, it facilitates tumor diagnostics without tissue resection. Furthermore, thanks to its non tissue destructive characteristics, e-biopsy enables probing the solid tumor multiple times in several distinct locations in the same procedure, thereby enabling the spatial profiling of tumor molecular heterogeneity.We demonstrate e-biopsy in vivo, using the 4T1 breast cancer model in mice to assess its performance, as well as the inferred spatial differential protein expression. In particular, we show that proteomic profiles obtained via e-biopsy in vivo distinguish the tumors from healthy breast tissue and reflect spatial tumor differential protein expression. E-biopsy provides a completely new molecular sampling modality for solid tumors molecular cartography, providing information that potentially enables more rapid and sensitive detection at lesser risk, as well as more precise personalized medicine.
A novel integrated MADM method for design concept evaluation
Authors: Chen Zhe , Zhong Peisi , Liu Mei , Ma Qing , Si Guangyao 
Published: September 2022

AbstractDesign concept evaluation plays a significant role in new product development. Rough set based methods are regarded as effective evaluation techniques when facing a vague and uncertain environment and are widely used in product research and development. This paper proposed an improved rough-TOPSIS method, which aims to reduce the imprecision of design concept evaluation in two ways. First, the expert group for design concept evaluation is classified into three clusters: designers, manufacturers, and customers. The cluster weight is determined by roles in the assessment using a Multiplicative Analytic Hierarchy Process method. Second, the raw information collection method is improved with a 3-step process, and both design values and expert linguistic preferences are integrated into the rough decision matrix. The alternatives are then ranked with a rough-TOPSIS method with entropy criteria weight. A practical example is shown to demonstrate the method’s viability. The findings suggest that the proposed decision-making process is effective in product concept design evaluation.
Impact of using different-sized touch keyboards on free-text keystroke dynamics authentication in the Arabic language
Authors: Alsuhibany Suliman A. , Almuqbil Afnan S. 
Published: September 2022

AbstractAuthentication using keystroke dynamics (KD) has become an active research area due to its usability and security aspects. Nowadays, the scale of touch keyboard use has expanded to include most modern devices. Although KD typically focuses on a single device at a particular time, authentication systems are adjustable within their environments, as these systems’ users frequently switch between multiple devices. Thus, this paper assesses users’ typing behavior on different tablet devices with varying touch keyboard sizes. In particular, we empirically assess whether the validation results from free-text KD authentication vary depending on the touch keyboard size. The results reveal interesting research directions for future feasibility studies on changing the dynamic keystroke typing-pad effect on user-security and trust-authentication analysis.
Post-disaster building damage assessment based on improved U-Net
Authors: Deng Liwei , Wang Yue 
Published: September 2022

AbstractWhen a severe natural disaster occurs, the extraction of post-disaster building damage information is one of the methods to quickly obtain disaster information. The increasingly mature high-resolution remote sensing technology provides a solid foundation for obtaining information about building damage. To address the issues with inaccurate building positioning in existing building damage assessment methods, as well as poor classification due to similar minor and major damage characteristics in building damage classification. Based on U-Net, we designed a two-stage building damage assessment network. The first stage is an independent U-Net focused on building segmentation, followed by a Siamese U-Net focused on building damage classification. The Extra Skip Connection and Asymmetric Convolution Block were used for enhancing the network's ability to segment buildings on different scales; Shuffle Attention directed the network's attention to the correlation of buildings before and after the disaster. The xBD dataset was used for training and testing in the study, and the overall performance was evaluated using a balanced F-score (F1). The improved network had an F1 of 0.8741 for localization and F1 of 0.7536 for classification. When compared to other methods, it achieved better overall performance for building damage assessment and was able to generalize to multiple disasters.
Assessment of risk factors for delayed gastric emptying after distal gastrectomy for gastric cancer
Authors: Mukoyama Tomosuke , Kanaji Shingo , Sawada Ryuichiro , Harada Hitoshi , Urakawa Naoki , Goto Hironobu , Hasegawa Hiroshi , Yamashita Kimihiro , Matsuda Takeru , Oshikiri Taro , Kakeji Yoshihiro 
Published: September 2022

AbstractThe risk factors for delayed gastric emptying (DGE) following gastrectomy are unclear. This study aimed to investigate the risk factors for DGE and the severity of DGE. We retrospectively evaluated 412 patients who underwent gastrectomy for gastric cancer between 2011 and 2019. The cases were classified into the DGE (n = 27) and non-DGE (n = 385) groups; the DGE group was further classified into two subgroups based on nasogastric tube insertion as an indicator of severity. For determining the relationship between resected stomach volume and DGE, we calculated the area of each surgical specimen using the ImageJ software. Female sex (odds ratio [OR] 2.55; 95% confidence interval [CI] 1.09–5.93; P = 0.03), diabetes (OR 2.38; 95% CI 1.02–5.57; P = 0.03), and distal gastric tumors (OR 2.61; 95% CI 1.10–6.19; P = 0.02) were identified as independent risk factors by multivariate analysis. The duration of hospital stay was longer in the DGE group than in the non-DGE group (29 vs. 15 days, P < 0.01). Overall, 24 cases of DGE (89%) were found in more than 1 week following surgery. No correlation was observed between clinical features and the severity of DGE. The resected area in the DGE group was significantly larger than that in the non-DGE group (198.0 vs. 173.9 cm2, P = 0.03). In conclusion, DGE was frequently observed in females and in patients with diabetes and distal gastric tumors. Most of the DGE cases occurred after 7–14 days of surgery, patients who are discharged early should be informed to seek hospitalization if they have symptoms caused by DGE.
Water availability and response of Tarbela Reservoir under the changing climate in the Upper Indus Basin, Pakistan
Authors: Khan Firdos 
Published: September 2022

AbstractAgriculture is one of the major contributors to the Gross Domestic Product (GDP) of Pakistan which relies on the availability of water. Hydropower contributes approximately 35% to the national electricity gid of Pakistan. Indus River is the main river of the Indus River System (IRS) which provides water for agriculture, hydropower and other purposes. The outputs of the Conformal-Cubic Atmospheric Model (CCAM) are used to force the University of British Columbia Watershed Model (UBCWM) in the Upper Indus Basin (UIB), to investigate future water availability under the two IPCC emission scenarios (RCP4.5 and RCP8.5). Tarbela Reservoir which is the outlet of UIB is used as a measurement tool to assess water availability and response of the reservoir to climate change. The results show that maximum and minimum temperature are increasing in the future in comparison to the reference period. The largest increases in maximum temperature are projected for MAM (March–April–May) and JJA (June–July–August), with increases up to 2 °C in MAM and increases up to 6.4 °C in JJA under the RCP4.5 and RCP8.5, respectively, in the future. Minimum temperature has maximum increase (6.7 °C) in DJF (December–January–February) during 2071–2100 under RCP8.5. Precipitation shows a 5.1% decrease in DJF during 2011–2040 under RCP4.5. The statistics about water availability suggest that there is consistent increase in most of the months in the future, however, under the RCP4.5, there is decline in the river flow during 2071–2100 as compared to the 2041–2070. The findings of this study show that most of the time there will be more water available but in some months, there may be water scarcity under the RCP4.5, however, proper management and optimal utilization can reduce the water scarcity.