bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulatory function of DNA methylation mediated lncRNAs in gastric cancer
Authors: Li Nan , Zeng Anqi , Wang Qian , Chen Maohua , Zhu Shaomi , Song Linjiang 
Published: July 2022

AbstractAs one of the most common malignancies worldwide, gastric cancer contributes to cancer death with a high mortality rate partly responsible for its out-of-control progression as well as limited diagnosis. DNA methylation, one of the epigenetic events, plays an essential role in the carcinogenesis of many cancers, including gastric cancer. Long non-coding RNAs have emerged as the significant factors in the cancer progression functioned as the oncogene genes, the suppressor genes and regulators of signaling pathways over the decade. Intriguingly, increasing reports, recently, have claimed that abnormal DNA methylation regulates the expression of lncRNAs as tumor suppressor genes in gastric cancer and lncRNAs as regulators could exert the critical influence on tumor progression through acting on DNA methylation of other cancer-related genes. In this review, we summarized the DNA methylation-associated lncRNAs in gastric cancer which play a large impact on tumor progression, such as proliferation, invasion, metastasis and so on. Furthermore, the underlying molecular mechanism and signaling pathway might be developed as key points of gastric cancer range from diagnosis to prognosis and treatment in the future.
Two lymphoid cell lines potently silence unintegrated HIV-1 DNAs
Authors: Geis Franziska K. , Kelenis Demetra P. , Goff Stephen P. 
Published: July 2022

AbstractMammalian cells mount a variety of defense mechanisms against invading viruses to prevent or reduce infection. One such defense is the transcriptional silencing of incoming viral DNA, including the silencing of unintegrated retroviral DNA in most cells. Here, we report that the lymphoid cell lines K562 and Jurkat cells reveal a dramatically higher efficiency of silencing of viral expression from unintegrated HIV-1 DNAs as compared to HeLa cells. We found K562 cells in particular to exhibit an extreme silencing phenotype. Infection of K562 cells with a non-integrating viral vector encoding a green fluorescent protein reporter resulted in a striking decrease in the number of fluorescence-positive cells and in their mean fluorescence intensity as compared to integration-competent controls, even though the levels of viral DNA in the nucleus were equal or in the case of 2-LTR circles even higher. The silencing in K562 cells was functionally distinctive. Histones loaded on unintegrated HIV-1 DNA in K562 cells revealed high levels of the silencing mark H3K9 trimethylation and low levels of the active mark H3 acetylation, as detected in HeLa cells. But infection of K562 cells resulted in low H3K27 trimethylation levels on unintegrated viral DNA as compared to higher levels in HeLa cells, corresponding to low H3K27 trimethylation levels of silent host globin genes in K562 cells as compared to HeLa cells. Most surprisingly, treatment with the HDAC inhibitor trichostatin A, which led to a highly efficient relief of silencing in HeLa cells, only weakly relieved silencing in K562 cells. In summary, we found that the capacity for silencing viral DNAs differs between cell lines in its extent, and likely in its mechanism.
Alkali treatment–acid leaching of rare earth elements from phosphogypsum fertilizer: insight for additional resource of valuable components
Authors: Gasser M. S. , Ismail Z. H. , Abu Elgoud E. M. , Hai F. Abdel , Ali I. O. , Aly H. F. 
Published: July 2022

AbstractPhosphogypsum (PG) is the main by-product of phosphoric acid, which is produced by the sulfuric acid attack of phosphate rocks, wet process. This by-product, which contains around 2.0% phosphoric acid, is used as a low-cost soil fertilizer, PGF. PGF consists mainly of gypsum (CaSO4·2H2O), P2O5, SiO2, and other impurities, including a minor amount of rare earth elements, REEs. In general, phosphate rocks contain from about 0.04 to 1.0% REE, which are precipitated with PG. Now, REEs are considered as strategic elements. Therefore, PG is now regarded as a secondary source of REE. This paper address a process for the separation of REEs and sodium sulphate as a product from PGF. This paper is based on the metathesis of the bulk of PGF with sodium carbonate to obtain calcium carbonate precipitated contain REEs. Furthermore, sodium sulphate was obtained as a product. Calcium carbonate containing REEs was leached out by citric acid as a green acid or nitric acid. At optimum conditions, maximum leaching of REEs from CaCO3 after one cycle of leaching by 3.0 mol/L nitric acid at L/S = 3/1, agitation time of 180.0 min., and at a temperature of 25 °C is 75.1%, 361.10 mg/kg from the total REEs present in PGF. While, the maximum leaching of 87.4%, 420.2 mg/kg of REEs from CaCO3 after one cycle of leaching by 1.0 mol/L citric acid, L/S = 5/1, agitation time of 15.0 min., and 85 °C. The REEs that were obtained in the leaching citrate solutions were purified by solvent extraction using 10% of di-2-ethyl hexyl phosphoric acid, HDEHP, in kerosene. The extracted REEs were stripped by 0.5 mol/L H2SO4. The stripped solutions were further treated with 10.0% oxalic acid to precipitate the REEs. The developed procedure can recover REEs from PGF with an efficiency of 85.2% and a purity of 97.7%.
Characterization and manipulation of the bacterial community in the midgut of Ixodes ricinus
Authors: Guizzo Melina Garcia , Dolezelikova Kristyna , Neupane Saraswoti , Frantova Helena , Hrbatova Alena , Pafco Barbora , Fiorotti Jessica , Kopacek Petr , Zurek Ludek 
Published: July 2022

Abstract
Background
Ticks are obligate hematophagous arthropods transmitting a wide range of pathogens to humans and animals. They also harbor a non-pathogenic microbiota, primarily in the ovaries and the midgut. In the previous study on Ixodes ricinus, we used a culture-independent approach and showed a diverse but quantitatively poor midgut bacterial microbiome. Our analysis also revealed the absence of a core microbiome, suggesting an environmental origin of the tick midgut microbiota.

Methods
A bacterial analysis of the midgut of adult females collected by flagging from two localities in the Czech Republic was performed. Using the culture-independent approach, we tested the hypothesis that the midgut microbiome is of the environmental origin. We also cultured indigenous bacteria from the tick midgut and used these to feed ticks artificially in an attempt to manipulate the midgut microbiome.

Results
The midgut showed a very low prevalence and abundance of culturable bacteria, with only 37% of ticks positive for bacteria. The culture-independent approach revealed the presence of Borrelia sp., Spiroplasma sp., Rickettsia sp., Midichloria sp. and various mainly environmental Gram-positive bacterial taxa. The comparison of ticks from two regions revealed that the habitat influenced the midgut bacterial diversity. In addition, the midgut of ticks capillary fed with the indigenous Micrococcus luteus (Gram-positive) and Pantoea sp. (Gram-negative) could not be colonized due to rapid and effective clearance of both bacterial taxa.

Conclusions
The midgut microbiome of I. ricinus is diverse but low in abundance, with the exception of tick-borne pathogens and symbionts. The environment impacts the diversity of the tick midgut microbiome. Ingested extracellular environmental bacteria are rapidly eliminated and are not able to colonize the gut. We hypothesize that bacterial elimination triggered in the midgut of unfed adult females is critical to maintain low microbial levels during blood-feeding.

Graphical Abstract
Noninvasive early detection of colorectal cancer by hypermethylation of the LINC00473 promoter in plasma cell-free DNA
Authors: Ruiz-Bañobre Juan , Rodriguez-Casanova Aitor , Costa-Fraga Nicolas , Bao-Caamano Aida , Alvarez-Castro Ana , Carreras-Presas Martín , Brozos-Vazquez Elena , Vidal-Insua Yolanda , Vazquez-Rivera Francisca , Candamio-Folgar Sonia , Mosquera-Presedo Manuel , Lago-Lestón Ramón M. , Muinelo-Romay Laura , Vázquez-Bueno José Ángel , Sanz-Pamplona Rebeca , Moreno Víctor , Goel Ajay , Castillo Lourdes , Martin Ana C. , Arroyo Rocio , Esteller Manel , Crujeiras Ana B. , López-López Rafael , Díaz-Lagares Angel 
Published: July 2022

Abstract
Background
Current noninvasive assays have limitations in the early detection of colorectal cancer. We evaluated the clinical utility of promoter methylation of the long noncoding RNA LINC00473 as a noninvasive biomarker to detect colorectal cancer and associated precancerous lesions.

Methods
We evaluated the epigenetic regulation of LINC00473 through promoter hypermethylation in colorectal cancer cell lines using bisulfite genomic sequencing and expression analyses. DNA methylation of LINC00473 was analyzed in primary colorectal tumors using 450K arrays and RNA-seq from The Cancer Genome Atlas (TCGA). Tissue-based findings were validated in several independent cohorts of colorectal cancer and advanced colorectal polyp patients by pyrosequencing. We explored the clinical utility of LINC00473 methylation for the early detection of colorectal cancer in plasma cell-free DNA by quantitative methylation-specific PCR and droplet digital PCR.

Results
LINC00473 showed transcriptionally silencing due to promoter hypermethylation in colorectal cancer cell lines and primary tumors. Methylation of the LINC00473 promoter accurately detected primary colorectal tumors in two independent clinical cohorts, with areas under the receiver operating characteristic curves (AUCs) of 0.94 and 0.89. This biomarker also identified advanced colorectal polyps from two other tissue-based clinical cohorts with high diagnostic accuracy (AUCs of 0.99 and 0.78). Finally, methylation analysis of the LINC00473 promoter in plasma cell-free DNA accurately identified patients with colorectal cancer and advanced colorectal polyps (AUCs of 0.88 and 0.84, respectively), which was confirmed in an independent cohort of patients.

Conclusions
Hypermethylation of the LINC00473 promoter is a new promising biomarker for noninvasive early detection of colorectal cancer and related precancerous lesions.
Robustness of the long-term nonlinear evolution of global sea surface temperature trend
Authors: Xu Zhenhao , Huang Gang , Ji Fei , Liu Bo , Chang Fei , Li Xichen 
Published: July 2022

AbstractThe multi-scale variability of global sea surface temperature (GSST), which is often dominated by secular trends, significantly impacts global and regional climate change. Previous studies were mainly carried out under linear assumptions. Even if the nonlinear evolution patterns have been discussed based on annual-mean data, the conclusions are still insufficient due to several factors. Here, based on the Ensemble Empirical Mode Decomposition (EEMD) method, the robustness of GSST trends tied to the sampling frequency and time interval selection is further explored. The main features derived from the annual-mean data are maintained. However, monthly and seasonal-mean data both mute the cooling in the equatorial central Pacific and the Southern Ocean in the Pacific sector, meanwhile intensify and expand the warming over the North Pacific. The results also highlight that early data cause a minimal effect on secular trends except for the portion near the start point of the interval due to the local temporal nature of EEMD. Overall, the long-term GSST trends extracted by EEMD have good robustness. Our research also clarifies that quadratic fitting cannot reveal all the meaningful evolution patterns, even as a nonlinear solution.
Nanoscale fluorescence imaging of biological ultrastructure via molecular anchoring and physical expansion
Authors: Wang Wei , Chan Yat Ho , Kwon SoYoung , Tandukar Jamuna , Gao Ruixuan 
Published: July 2022

AbstractNanoscale imaging of biological samples can provide rich morphological and mechanistic information about biological functions and dysfunctions at the subcellular and molecular level. Expansion microscopy (ExM) is a recently developed nanoscale fluorescence imaging method that takes advantage of physical enlargement of biological samples. In ExM, preserved cells and tissues are embedded in a swellable hydrogel, to which the molecules and fluorescent tags in the samples are anchored. When the hydrogel swells several-fold, the effective resolution of the sample images can be improved accordingly via physical separation of the retained molecules and fluorescent tags. In this review, we focus on the early conception and development of ExM from a biochemical and materials perspective. We first examine the general workflow as well as the numerous variations of ExM developed to retain and visualize a broad range of biomolecules, such as proteins, nucleic acids, and membranous structures. We then describe a number of inherent challenges facing ExM, including those associated with expansion isotropy and labeling density, as well as the ongoing effort to address these limitations. Finally, we discuss the prospect and possibility of pushing the resolution and accuracy of ExM to the single-molecule scale and beyond.
Producing Korean literature (KLit) for export
Authors: Levitt Peggy , Shim Bo-Seon 
Published: July 2022

AbstractHow does art from what have been culturally peripheral countries that were not former colonies of Western powers scale shift or find its way to the global center? What can the Korean case tell us about the circulation of contemporary literature in a “small language?” The scholarly literature offers many answers to these questions: the role of intermediaries, the power dynamics within the world system of translation, the topographies of literary circulation, and a range of other political, cultural, economic, and social factors. We propose that the Korean case sheds new light on these discussions in several important ways loosely subsumed under the umbrella of infrastructures—the platforms, passageways, containers, and gates that organize the writing, reading, publishing, and marketing of the literature. We see three kinds of infrastructures as catalysts of Korean literary success including infrastructures of export and promotion, infrastructures of discovery and consecration, and infrastructures of connection and vernacularization.
The influence of psychological interventions on surgical outcomes: a systematic review
Authors: Lanini Iacopo , Amass Timothy , Calabrisotto Caterina Scirè , Fabbri Sergio , Falsini Silvia , Adembri Chiara , Di Filippo Alessandro , Romagnoli Stefano , Villa Gianluca 
Published: July 2022

Abstract
Background
An amplified and/or prolonged surgical stress response might overcome the organs’ functional reserve, thus leading to postoperative complications. The aim of this systematic literature review is to underline how specific psychological interventions may contribute to improve surgical outcomes through the positive modulation of the surgical stress response in surgical patients.

Methods
We conducted a comprehensive literature search in the Cochrane Register of Controlled Trials, PubMed, EMBASE, Scopus, PsycINFO, and CINAHL databases. Only studies published in English from Jan 2000 to Apr 2022 and reporting pain and/or anxiety among outcome measures were included in the review. The following psychological interventions were considered: (1) relaxation techniques, (2) cognitive-behavioral therapies, (3) mindfulness, (4) narrative medicine, (5) hypnosis, and (6) coping strategies.

Results
Among 3167 records identified in the literature, 5 papers were considered eligible for inclusion in this review because reporting the effects that psychological features have on neurochemical signaling during perioperative metabolic adaptation and those metabolic and clinical effects that the psychological interventions had on the observed population.

Conclusion
Our findings confirm that psychological interventions may contribute to improve surgical outcomes via the positive influence on patients’ metabolic surgical stress response. A multidisciplinary approach integrating physical and non-physical therapies can be considered a good strategy to successfully improve surgical outcomes in the perioperative period.
Teprotumumab Divergently Alters Fibrocyte Gene Expression: Implications for Thyroid-associated Ophthalmopathy
Authors: Fernando Roshini , Smith Terry J 
Published: July 2022

Abstract

Context
Teprotumumab, an IGF-I receptor (IGF-IR) inhibitor, is effective in thyroid-associated ophthalmopathy (TAO). The drug can modulate induction by TSH of IL-6 and IL-8 in CD34+ fibrocytes and their putative derivatives, CD34+ orbital fibroblasts (CD34+ OF). Fibrocytes express multiple thyroid autoantigens and cytokines implicated in TAO, which are downregulated by Slit2. Inflammation and disordered hyaluronan (HA) accumulation occur in TAO. Whether teprotumumab alters these processes directly in fibrocytes/CD34+ OF remains uncertain.

Objective
Determine teprotumumab effects on expression/synthesis of several TAO-relevant molecules in fibrocytes and GD-OF.

Design/Setting/Participants
Patients with TAO and healthy donors were recruited from an academic endocrine and oculoplastic practice.

Main outcome measures
Real-time PCR, specific immunoassays.

Results
Teprotumumab attenuates basal and TSH-inducible autoimmune regulator protein, thyroglobulin, sodium iodide symporter, thyroperoxidase, IL-10, and B-cell activating factor levels in fibrocytes. It downregulates IL-23p19 expression/induction while enhancing IL-12p35, intracellular and secreted IL-1 receptor antagonists, and Slit2. These effects are mirrored by linsitinib. HA production is marginally enhanced by teprotumumab, the consequence of enhanced HAS2 expression.

Conclusion
Teprotumumab affects specific gene expression in fibrocytes and GD-OF in a target-specific, nonmonolithic manner, whereas IGF-IR control of these cells appears complex. The current results suggest that the drug may act on cytokine expression and HA production systemically and locally, within the TAO orbit. These findings extend our insights into the mechanisms through which IGF-IR inhibition might elicit clinical responses in TAO, including a potential role of Slit2 in attenuating inflammation and tissue remodeling.
Physiological Effects of Single and Double Face Mask Use with Moderate and Vigorous Exercise
Authors: LaBotz Michele , Stroshine Karli , Dekker Ellie , Visich Paul 
Published: July 2022

ABSTRACT

Introduction/Purpose
Exercise is associated with increased exhalation of infectious particles in respiratory disease, and face mask use has become routine during the coronavirus disease 2019 (COVID-19) pandemic to mitigate particle spread. As of February 2022, the U.S. Centers for Disease Control and Prevention (CDC) continues to recommend the use of double masking (i.e., cloth masks worn over a surgical mask) to minimize particle leakage seen with single-mask use. Studies to date have examined the effects of single masks on exercise safety and performance, but the potential added burden during exercise of an additional mask layer has not been evaluated. The purpose of our study was to compare the effects of single and double face mask use during moderate to vigorous exercise.

Methods
Fifteen healthy, physically active young adults (mean age = 21.1 ± 0.8 yr; 8 males and 7 females; V˙O2peak = 46.3 ± 11.5 mL·kg−1⋅min−1) completed the study. This included an initial assessment of V˙O2peak, with subjects returning for three exercise test sessions performed while wearing either a surgical face mask, a cloth face mask, or double masks (i.e., a cloth mask worn over a surgical mask) in a random order. Test sessions consisted of a progressive cycling protocol with 10-min stages at 40%, 60%, and 75% oxygen consumption reserve (VO2R). Assessments were performed after 5 and 10 min at each exercise intensity and included heart rate, respiratory rate, oxygen saturation, and ratings of perceived exertion and dyspnea.

Results
At exercise intensities from 40% to 75% VO2R, there were no significant differences between cloth masks, surgical masks, and double masking in the measured subjective and physiologic parameters.

Conclusions
When compared with surgical and cloth face masks in common use, for the outcomes measured in this study, double masking in accordance with CDC recommendations does not result in significant impairments during moderate to vigorous exercise up to 75% VO2R in healthy young adults.
Osteoporosis screening support system from panoramic radiographs using deep learning by convolutional neural network
Authors: Nakamoto Takashi , Taguchi Akira , Kakimoto Naoya 
Published: July 2022

Objectives: This study was performed to develop computer-aided screening systems that could predict osteoporosis. The systems were constructed using panoramic radiographs of women aged ≥ 50 years through three types of deep convolutional neural networks (CNNs): Alexnet, VGG-16, and GoogLeNet; the performances of the constructed systems were evaluated. Methods: One oral radiologist classified 1500 panoramic radiographs into three types. In C1, the endosteal margin of the cortex was smooth and sharp, whereas porosities were observed in C2 and C3. The risks of osteoporosis were higher in C2 and C3 than in C1; C3 had the highest risk. This information was included with the images as training data; three CNNs were transfer trained. Using each trained CNN, the diagnostic accuracy was assessed using panoramic radiographs and bone mineral density inspection findings in the lumbar spine and femoral neck of 100 additional patients. Results: All CNNs exhibited relatively good agreement with the oral radiologist’s judgement (86.0%–90.7%). The predictive results of the three systems for osteoporosis of the lumbar spine showed sensitivities of 78.3%–82.6%, specificities of 71.4%–79.2%, and accuracies of 74.0%–79.0%. The predictive results for osteoporosis of the femoral neck showed sensitivities of 80.0%–86.7%, specificities of 67.1%–74.1%, and accuracies of 70.0%–75.0%. Conclusions: The constructed systems were generally more accurate than the previously developed conventional system. The new systems may facilitate osteoporosis prediction and prevent subsequent fractures by encouraging patients with suspected osteoporosis to undergo further inspections (e.g., dual-energy X-ray absorptiometry) and treatment.
PEDAGOGIA ANTIRRACISTA E PROCESSOS IDENTITÁRIOS: TEORIA E PRÁTICA NA EDUCAÇÃO INFANTIL
Authors: Amorim Juliana Pires Pessôa 
Published: July 2022

Este artigo tem como tema Pedagogia Antirracista e Educação Infantil, e configura os primeiros registros de pesquisa iniciada para fins de trabalho de conclusão do curso Ações Afirmativas e Políticas Públicas (ProDEd - TS/UERJ). Enquanto mãe de criança pequena, servidora em escola pública e futura pedagoga, deparei-me diversas vezes com padrões racistas como obstáculo ao cumprimento dos princípios previstos na Constituição (BRASIL, 1988) e na LDB (BRASIL, 1996). Assim, a fim de analisar as consequências de práticas pedagógicas antirracistas nos processos identitários na Educação Infantil, a metodologia utilizada foi revisão bibliográfica alinhada a estudo de caso. Na primeira seção, a proposta é conceituar educação antirracista; na segunda, apresentar nuances dos processos identitários infantis; e na terceira, relacionar teoria e prática mediante estudo de caso. Finalmente, a associação entre estudos relacionando questões raciais e Educação Infantil à prática docente de uma professora integrante de NEABI encaminhou as considerações finais em direção a constatações, alternativas e indagações para a continuidade da pesquisa futuramente.