bluuebunny/crossref_metadata_2025_split huggingface dataset:
Innervation of the Female Breast and Nipple: A Systematic Review and Meta-Analysis of Anatomical Dissection Studies
Authors: Smeele Hansje P. , Bijkerk Ennie , van Kuijk Sander M. J. , Lataster Arno , van der Hulst René R. W. J. , Tuinder Stefania M. H. 
Published: June 2022

Background:
Primary cadaveric studies were reviewed to give a contemporary overview of what is known about innervation of the female breast and nipple/nipple-areola complex.

Methods:
The authors performed a Preferred Reporting Items for Systematic Reviews and Meta-Analyses–compliant systematic review and meta-analysis. The authors searched four electronic databases for studies investigating which nerve branches supply the female breast and nipple/nipple-areola complex or describing the trajectory and other anatomical features of these nerves. Inclusion criteria for meta-analysis were at least five studies of known sample size and with numerical observed values. Pooled prevalence estimates of nerve branches supplying the nipple/nipple-areola complex were calculated using random-effects meta-analyses; the remaining results were structured using qualitative synthesis. Risk of bias within individual studies was assessed with the Anatomical Quality Assurance checklist.

Results:
Of 3653 studies identified, 19 were eligible for qualitative synthesis and seven for meta-analysis. The breast skin is innervated by anterior cutaneous branches and lateral cutaneous branches of the second through sixth and the nipple/nipple-areola complex primarily by anterior cutaneous branches and lateral cutaneous branches of the third through fifth intercostal nerves. The anterior cutaneous branch and lateral cutaneous branch of the fourth intercostal nerve supply the largest surface area of the breast skin and nipple/nipple-areola complex. The lateral cutaneous branch of the fourth intercostal nerve is the most consistent contributory nerve to the nipple/nipple-areola complex (pooled prevalence, 89.0 percent; 95 percent CI, 0.80 to 0.94).

Conclusions:
The anterior cutaneous branch and lateral cutaneous branch of the fourth intercostal nerve are the most important nerves to spare or repair during reconstructive and cosmetic breast surgery. Future studies are required to elicit the course of dominant nerves through the breast tissue.
Magnetic Resonance Imaging Screening after Silicone Implant Breast Surgery: Patient Survey of Adherence to U.S. Food and Drug Administration Recommendations
Authors: Copeland-Halperin Libby R. , Wampler Ariel T. , Doughty Hayden , Shank Nina , Levy Joshua J. , Reategui Via y Rada Maria L. , Rosen Joseph M. 
Published: June 2022

Background:
The U.S. Food and Drug Administration recommends women with silicone breast implants undergo magnetic resonance imaging surveillance to detect asymptomatic rupture. Screening is costly and often not covered by insurance. The authors assessed awareness of and adherence to these recommendations among patients with silicone breast implants.

Methods:
The authors searched electronic medical records for patients aged 18 years or older with silicone breast implants placed between 2011 and 2016. Consenting patients were surveyed by telephone using a standardized script to assess awareness of U.S. Food and Drug Administration recommendations, whether they had undergone magnetic resonance imaging screening, and barriers to testing. Patients who declined to participate or could not be contacted were excluded. Demographics and operative data were collected. Odds ratios were calculated with one-sample 95 percent confidence intervals, and Fisher exact tests of independence were conducted under assumptions of normality. Multivariable logistic regression analysis was performed to test for confounding.

Results:
Of 370 patients meeting inclusion criteria, 109 consented to participate. Adherence to U.S. Food and Drug Administration recommendations was 5.9 percent (95 percent CI, 0.15 to 28.7 percent). There was no difference in adherence between patients undergoing cosmetic versus reconstructive surgery (p = 1.00; OR, 0.80; 95 percent CI, 1.17 to 2.93), having health insurance (p = 0.58), or residing in a county with median annual household income greater than that of the state of residence (p = 0.33).

Conclusions:
A small proportion of respondents had undergone magnetic resonance imaging in accordance with U.S. Food and Drug Administration recommendations. Low adherence highlights a potential limitation of current federal surveillance recommendations. Additional research is needed to better characterize adherence to magnetic resonance imaging surveillance recommendations, identify barriers to implementation, and determine whether this recommendation remains valid.
HIV Risk Factors and Risk Perception Among Adolescent Girls and Young Women: Results From a Population-Based Survey in Western Kenya, 2018
Authors: Kamire Vivienne , Magut Faith , Khagayi Sammy , Kambona Caroline , Muttai Hellen , Nganga Lucy , Kwaro Daniel , Joseph Rachael H. 
Published: June 2022

Background:
In sub-Saharan Africa, HIV prevalence in adolescent girls and young women (AGYW) is 2-fold to 3-fold higher than that in adolescent boys and young men. Understanding AGYW's perception of HIV risk is essential for HIV prevention efforts.

Methods:
We analyzed data from a HIV biobehavioral survey conducted in western Kenya in 2018. Data from AGYW aged 15–24 years who had a documented HIV status were included. We calculated weighted prevalence and evaluated factors associated with outcomes of interest (HIV infection and high risk perception) using generalized linear models to calculate prevalence ratios.

Results:
A total of 3828 AGYW were included; 63% were aged 15–19 years. HIV prevalence was 4.5% and 14.5% of sexually active AGYW had high risk perception. Over 70% of participants had accessed HIV testing and counseling in the past 12 months. Factors associated with both HIV infection and high risk perception included having an HIV-positive partner or partner with unknown status and having a sexually transmitted infection in the past 12 months. Having an older (by ≥10 years) partner was associated with HIV infection, but not high risk perception. Less than 30% of sexually active AGYW with 3 or more HIV risk factors had high perception of HIV risk.

Conclusion:
Gaps in perceived HIV risk persist among AGYW in Kenya. High access to HIV testing and prevention services in this population highlights platforms through which AGYW may be reached with improved risk counseling, and to increase uptake of HIV prevention strategies.
Postoperative Utilization of Opioids After Midurethral Sling Surgery: A Prospective Clinical Practice Study
Authors: Ramaseshan Aparna S. , O'Meara Amanda , O'Sullivan David M. , Tunitsky-Bitton Elena 
Published: June 2022

Importance
This study was performed to understand opioid consumption after midurethral sling (MUS) procedures to provide surgeons with guidelines on appropriate prescription regimens.

Objective
This study aimed to evaluate postdischarge narcotic use (PDNU) after MUS surgery using a restrictive postdischarge opioid regimen.

Study Design
This prospective clinical practice study included women undergoing MUS surgery from December 2018 to October 2019. Patients were discharged with an electronic prescription for 5 tablets of an oral narcotic. Brief Pain Inventory (BPI) surveys were collected preoperatively. Patients answered questions regarding remaining number of opioid tablets, BPI scores, and patient satisfaction on day 1, week 1 (postoperative week [POW] 1), and 4–6 weeks (POW4–6) postoperatively. Electronic records were reviewed to determine narcotic refills. Primary outcome was PDNU measured in morphine milligram equivalents (MME). Secondary outcomes evaluated refill rate, BPI scores, and patient satisfaction. Standard statistical tests were applied.

Results
Fifty-six patients were included in the analysis. Total median (interquartile range) PDNU was 12.5 (0–37.5) MME at POW1 and 15.0 (0–37.5) MME at POW4–6, which is approximately equivalent to 2 oxycodone (5 mg) tablets. Eighteen patients (32.1%) took 0 narcotics postdischarge. Brief Pain Inventory scores showed that “worst pain” was highest on postoperative day 1 with a median (interquartile range) score of 6 (4–8). At the POW1 and POW4–6 mark, 94.3% and 92% of patients, respectively, were satisfied or extremely satisfied with their pain control. Seven patients (11.8%) required a refill.

Conclusions
Most patients undergoing MUS surgery used no more than 2 oxycodone (5 mg) tablets during their 6-week postoperative course, with almost a third not requiring any narcotics. Low pain scores and high patient satisfaction rates were noted.
Barriers and Promotors to Health Service Utilization for Pelvic Floor Disorders in the United States: Systematic Review and Meta-analysis of Qualitative and Quantitative Studies
Authors: Mou Tsung , Gonzalez Javier , Gupta Ankita , O’Shea Michele , Thibault Mary Duarte , Gray Elizabeth L. , Beestrum Molly , Brown Oluwateniola , Cichowski Sara 
Published: June 2022

Importance
We need a systematic approach to understanding health service utilization behavior in women with pelvic floor symptoms in the United States.

Objectives
The aim of this study was to determine the prevalence of pelvic floor care utilization and identify its barriers and promotors using Andersen’s model, which theorizes care-seeking behavior with individual care needs, resources, predispositions, and macrostructures.

Design
This was a systematic search of studies on care-seeking patterns in women with symptomatic pelvic floor disorders, which included pelvic organ prolapse, lower urinary tract symptoms, and anal incontinence. We then performed meta-analyses with random-effects models and descriptive analysis to determine utilization rate and the impact of each identified determinant.

Results
The pooled utilization rate was 37% (95% confidence interval [CI], 30%–45%). Determinants were identified across all domains except at the macrostructure level. For individual care needs, increased symptom severity or duration and a history of depression (odds ratio [OR], 1.27; 95% CI, 1.07–1.51) were associated with higher utilization. In the resources domain, having social support and established primary/secondary care were promotors, whereas higher income and employed status trended as barriers. Under predispositions, age ≥50 years (OR, 1.29; 95% CI, 1.05–1.60), and pelvic floor awareness were associated with utilization, whereas Asian (OR, 0.60; 95% CI, 0.51–0.72) and Black (OR, 0.77; 95% CI, 0.55–1.08) women as well as women experiencing fear, misinformation, or normalization of pelvic floor disorders had decreased utilization.

Conclusions
This review identified a low utilization rate for pelvic floor care and revealed multilayered, actionable items affecting care-seeking behavior. It highlights the need for more inclusive and multifaceted approaches in future pelvic floor disparity research and equity interventions.
Genetic and epigenetic signatures associated with plasma oxytocin levels in children and adolescents with autism spectrum disorder
Authors: Siecinski Stephen K , Giamberardino Stephanie N , Spanos Marina , Hauser Annalise C , Gibson Jason R , Chandrasekhar Tara , Trelles M D Pilar , Rockhill Carol M , Palumbo Michelle L , Cundiff Allyson Witters , Montgomery Alicia , Siper Paige , Minjarez Mendy , Nowinski Lisa A , Marler Sarah , Kwee Lydia C , Shuffrey Lauren C , Alderman Cheryl , Weissman Jordana , Zappone Brooke , Mullett Jennifer E , Crosson Hope , Hong Natalie , Luo Sheng , She Lilin , Bhapkar Manjushri , Dean Russell , Scheer Abby , Johnson Jacqueline L , King Bryan H , McDougle Christopher J , Sanders Kevin B , Kim Soo-Jeong , Kolevzon Alexander , Veenstra-VanderWeele Jeremy , Hauser Elizabeth R , Sikich Linmarie , Gregory Simon G 
Published: June 2022

ABSTRACTOxytocin (OT), the brain’s most abundant neuropeptide, plays an important role in social salience and motivation. Clinical trials of the efficacy of OT in autism spectrum disorder (ASD) have reported mixed results due in part to ASD’s complex etiology. We hypothesized that genetic and epigenetic variation contribute to variable endogenous OT levels that modulate sensitivity to OT therapy. To test this hypothesis, we integrated genome-wide profiles of DNA-methylation, transcriptional activity, and genetic variation with plasma OT levels in 290 participants with ASD enrolled in a randomized controlled trial of OT. Our analysis shows subtle, but statistically significant association of plasma OT levels with peripheral transcriptional activity and DNA-methylation profiles across several annotated gene sets. We also identified genetic variants with novel association with plasma OT, several of which reside in known ASD risk genes. These findings broaden our understanding of the effects of the peripheral oxytocin system and provide novel genetic candidates for future studies to decode the complex etiology of ASD and its interaction with OT signaling and OT-based interventions.
Adipose-derived stromal cells preserve pancreatic islet function in a transplantable 3D bioprinted scaffold
Authors: Abadpour Shadab , Niemi Essi M. , Orrhult Linnea Strid , Hermanns Carolin , de Vries Rick , Nogueira Liebert Parreiras , Haugen Håvard Jostein , Josefsen Dag , Krauss Stefan , van Apeldoorn Aart , Gatenholm Paul , Scholz Hanne 
Published: June 2022

AbstractIntra-portal islet transplantation is the method of choice for treatment of insulin dependent type 1 diabetes, but its outcome is hindered by limited islet survival due to the immunological and metabolic stress post transplantation. Adipose-derived stromal cells (ASCs) promise to improve significantly the islet micro-environment but an efficient long-term delivery method has not been achieved. We therefore explore the potential of generating ASC enriched islet transplant structure by 3D bioprinting. Here, we fabricate a double-layered 3D bioprinted scaffold for islets and ASCs by using alginate-nanofibrillated cellulose bioink. We demonstrate the diffusion properties of the scaffold and report that human ASCs increase the islet viability, preserve the endocrine function, and reduce pro-inflammatory cytokines secretionin vitro. Intraperitoneal implantation of the ASCs and islets in 3D bioprinted scaffold improve the long-term function of islets in diabetic mice. Our data reveals an important role for ASCs on the islet micro-environment. We suggest a novel cell therapy approach of ASCs combined with islets in a 3D structure with a potential for clinical beta cell replacement therapies at extrahepatic sites.
Are Brain Responses to Emotion a Reliable Endophenotype of Schizophrenia? An Image-based fMRI Meta-analysis
Authors: Fiorito Anna M. , Aleman André , Blasi Giuseppe , Bourque Josiane , Cao Hengyi , Chan Raymond C. K. , Chowdury Asadur , Conrod Patricia , Diwadkar Vaibhav A. , Goghari Vina M. , Guinjoan Salvador , Gur Raquel E. , Gur Ruben C. , Kwon Jun Soo , Lieslehto Johannes , Lukow Paulina B. , Meyer-Lindenberg Andreas , Modinos Gemma , Quarto Tiziana , Spilka Michael J. , Shivakumar Venkataram , Venkatasubramanian Ganesan , Villarreal Mirta , Wang Yi , Wolf Daniel H. , Yun Je-Yeon , Fakra Eric , Sescousse Guillaume 
Published: June 2022

AbstractBackgroundImpaired emotion processing constitutes a key dimension of schizophrenia and a possible endophenotype of this illness. Empirical studies consistently report poorer emotion recognition performance in patients with schizophrenia as well as in individuals at enhanced risk of schizophrenia (“at risk”). fMRI studies also report consistent patterns of abnormal brain activation in response to emotional stimuli in patients, in particular decreased amygdala activation. In contrast, brain-level abnormalities in at-risk individuals are more elusive. We address this gap using an image-based meta-analysis of the fMRI literature.MethodsfMRI studies investigating brain responses to negative emotional stimuli and reporting a comparison between at-risk individuals and healthy controls were identified. Frequentist and Bayesian voxel-wise meta-analyses were performed separately, by implementing a random effect model with unthresholded group-level T-maps from individual studies as input.ResultsSeventeen studies with a cumulative total of 677 at-risk individuals and 805 healthy controls were included. Frequentist analyses did not reveal significant differences between at-risk individuals and healthy controls. Similar results were observed with Bayesian analyses, which provided strong evidence for the absence of meaningful brain activation differences across the entire brain. Region of interest analyses specifically focusing on the amygdala confirmed the lack of group differences in this region.ConclusionsThese results suggest that brain activation patterns in response to emotional stimuli are unlikely to constitute a reliable endophenotype of schizophrenia. We suggest that future studies rather focus on impaired functional connectivity as an alternative and promising endophenotype.
The global prevalence and ethnic heterogeneity of iron-refractory iron deficiency anaemia
Authors: Fan Shanghua , Zhao Ting , Sun Liu 
Published: June 2022

AbstractBackgroundIron-refractory iron deficiency anaemia (IRIDA) is an autosomal recessive iron deficiency anaemia caused by mutations in theTMPRSS6gene. Iron deficiency anaemia is common, whereas IRIDA is rare. The prevalence of IRIDA is unclear. This study aimed to estimate the carrier frequency and genetic prevalence of IRIDA using Genome Aggregation Database (gnomAD) data.MethodsThe pathogenicity ofTMPRSS6variants was interpreted according to the American College of Medical Genetics and Genomics (ACMG) and the Association for Molecular Pathology (AMP) standards and guidelines. The minor allele frequency (MAF) ofTMPRSS6gene disease-causing variants in 141456 unique individuals was examined to estimate the global prevalence of IRIDA in seven ethnicities: African/African American (afr), American Admixed/Latino (amr), Ashkenazi Jewish (asj), East Asian (eas), Finnish (fin), Non-Finnish European (nfe) and South Asian (sas). The global and population-specific carrier frequencies and genetic prevalence of IRIDA were calculated using the Hardy-Weinberg equation.ResultsIn total, 86 pathogenic/likely pathogenic variants (PV/LPV) were identified according to ACMG/AMP guideline. The global carrier frequency and genetic prevalence of IRIDA were 2.02 per thousand and 1.02 per million, respectively.ConclusionsThe prevalence of IRIDA is greater than previous estimates.
Levels and determinants of quality of antenatal care in Bangladesh: Evidence from the Bangladesh Demographic and Health Survey
Authors: Akter Ema , Hossain Aniqa Tasnim , Rahman Ahmed Ehsanur , Ahmed Anisuddin , Tahsina Tazeen , Tanwi Tania Sultana , Nusrat Nowrin , Nahar Quamrun , Arifeen Shams El , Chowdhury Mahbub Elahi 
Published: June 2022

AbstractBackgroundAssessing the quality of antenatal care (ANC) is imperative for improving care provisions during pregnancy to ensure the health of mother and baby. In Bangladesh, there is a dearth of research on the quality of ANC using nationally representative data to understand its levels and determinants. The current study aims to assess the quality of ANC and identify the sociodemographic factors associated with the usage of quality ANC services in Bangladesh.MethodsWe conducted secondary data analysis using the last two Bangladesh Demographic and Health Surveys (BDHS) (2014 and 2017–18). A total of 8,277 ever-married women were included in the analysis (3,631 from 2014 and 4,646 from 2017–18 BDHS). We constructed the quality ANC index using a principal component analysis on different ANC components: weight, blood pressure measurement, blood and urine tests, counseling about pregnancy complications and a minimum of four ANC visits of which one is by a medically trained provider. Multinomial logistic regression was used to determine the strength of association.ResultsReceiving all the six components of quality ANC increased from about 13% in 2014 (BDHS 2014) to 18% in 2017/18 (BDHS 2017–18) with a significant difference of p < 0.001. Women from the poorest group, being rural areas, with no education, high birth order and unexposed to media were less likely to receive high-quality ANC than women from the richest group, from urban areas, with a higher level of education, low birth order and exposure to media.ConclusionThere is a need to improve the quality of ANC services in Bangladesh. An education program for women, with regular knowledge-enhancing sessions for pregnant mothers, may help them understand the value of ANC visits. Documentaries about maternal and child healthcare can be broadcast on television, YouTube, Facebook, radio and other digital platforms regularly.
SARS-CoV-2 impacts the transcriptome and epigenome at the maternal-fetal interface in pregnancy
Authors: Gao Lin , Mathur Vrinda , Tam Sabrina Ka Man , Zhou Xuemeng , Cheung Ming Fung , Chan Lu Yan , Estrada-Gutiérrez Guadalupe , Leung Bo Wah , Moungmaithong Sakita , Wang Chi Chiu , Poon Liona C. , Leung Danny 
Published: June 2022

AbstractDuring pregnancy, the maternal-fetal interface plays vital roles in fetal development. Its disruption is frequently found in pregnancy complications. Recent works show increased incidences of adverse pregnancy outcomes in COVID-19 patients; however, the mechanism remains unclear. Here, we analyzed the molecular impacts of SARS-CoV-2 infection on the maternal-fetal interface. Generating bulk and single-nucleus transcriptomic and epigenomic profiles from COVID-19 patients and control samples, we discovered aberrant immune activation and angiogenesis patterns in patients. Surprisingly, retrotransposons were dysregulated in specific cell types. Notably, reduced enhancer activities of LTR8B elements were functionally linked to the downregulation of Pregnancy-Specific Glycoprotein genes in syncytiotrophoblasts. Our findings revealed that SARS-CoV-2 infection induced significant changes to the epigenome and transcriptome at the maternal-fetal interface, which may be associated with pregnancy complications.One-Sentence SummaryPregnant COVID-19 patients show placental epigenetic and transcriptional changes, associated with adverse pregnancy outcomes.
FBXO11 deficiency in mice impairs lung development and aggravates cigarette smoke-induced airway fibrosis
Authors: Shenoy Anitha , Luo Huacheng , Lulu Sarah , Li Jennifer W. , Jin Yue , Yu Qin , Guo Shu , Shenoy Vinayak , Chen Hao , Bryant Andrew , Wu Lizi , Chang Jia , Mohammed Kamal , Lu Jianrong 
Published: June 2022

AbstractSmall airway fibrosis is a common pathology of chronic obstructive pulmonary disease (COPD) and contributes to airflow obstruction. However, the underlying fibrogenic mechanism is poorly understood. Epithelial-mesenchymal transition (EMT) has been proposed as a driver of fibrosis. EMT occurs in the airways of COPD patients and smokers, but it remains elusive whether EMT may contribute to airway fibrosis. We previously reported that FBXO11 is a critical suppressor of EMT and Fbxo11 deficiency in mice causes neonatal lethality and EMT in epidermis. Here, we found that Fbxo11-deficient mouse embryonic lungs showed impaired epithelial differentiation, excess fibroblast cells surrounding the airways, and thickened interstitial mesenchyme. We further generated conditional mutant mice to ablate Fbxo11 selectively in the club airway epithelial cells in adult mice, which induced partial EMT in the airways. To determine the effect of EMT on airway fibrosis, Fbxo11 conditional mutant mice were exposed to cigarette smoke. Airway-specific loss of Fbxo11 markedly enhanced smoking-induced airway fibrotic remodeling and collagen deposition. Taken together, our study suggests that EMT in the airway epithelium exacerbates cigarette smoke-induced airway fibrosis.