bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA SNHG1 targets miR‐340‐5p/PIK3CA axis to regulate microvascular endothelial cell proliferation, migration, and angiogenesis in DR
Authors: He Fu‐Tao , Fu Xiao‐Lin , Li Mo‐Han , Fu Chun‐Yan , Chen Jian‐Zhi 
Published: December 2022

AbstractDiabetic retinopathy (DR) is a serious long‐term complication of diabetes. However, the current treatment of DR is still challenging. We aimed to investigate the role of lncRNA SNHG1/miR‐340‐5p/PIK3CA in DR and the mechanisms involved. Blood samples from clinical DR patients and healthy subjects were obtained. HRMECs were induced by high glucose for 24 h to establish the DR model. The vector for interfering or overexpressing lncRNA SNHG1, miR‐340‐5p, and PIK3CA was constructed. LncRNA SNHG1, miR‐340‐5p, and PIK3CA expressions were detected by qRT‐PCR or Western blot. Cell proliferation and migration were detected by CCK‐8 and Transwell assays. Blood vessel formation was detected by angiogenesis assay. Dual‐luciferase reporter assay tested the interaction of lncRNA SNHG1 with miR‐340‐5p and miR‐340‐5p with PIK3CA. RIP measured the binding of miR‐340‐5p to PIK3CA. In the blood of DR patients and the DR model, lncRNA SNHG1 was increased and miR‐340‐5p expression was down‐regulated. In the DR model, PIK3CA expression was elevated. Downregulation of lncRNA SNHG1 inhibited HRMECs proliferation, migration, and angiogenesis. LncRNA SNHG1 interacted with miR‐340‐5p, and up‐regulation of miR‐340‐5p inhibited HRMECs proliferation, migration and angiogenesis. The inhibition of cell proliferation, migration, and angiogenesis of HRMECs caused by down‐regulation of lncRNA SNHG1 was reversed by knockdown of miR‐340‐5p. miR‐340‐5p targeted PIK3CA, and downregulation of PIK3CA inhibited HRMECs proliferation, migration, and angiogenesis. The inhibition of HRMECs proliferation, migration and angiogenesis caused by down‐regulation of lncRNA SNHG1 could be reversed by overexpression of PIK3CA. LncRNA SNHG1 targeted miR‐340‐5p/PIK3CA axis to regulate microvascular endothelial cell proliferation, migration, and angiogenesis in DR.
Decades of weathering improves coal waste biogeochemical properties by altering dissolved organic matter and microbial communities: A case‐study of a coal waste pile
Authors: Tuo Pinpeng , Ren Jie , Hao Chongkai , Zhang Xi , Li Renyou 
Published: December 2022

AbstractThe spatial heterogeneity of vegetation cover in coal waste pile suggests that the geochemical and biological properties of coal waste are spatially heterogeneous, which contributes to vegetation colonization on tailings. However, whether and how altering the organic matter components and microbial community of coal waste via weathering affects biogeochemical characteristics and vegetation establishment mechanisms remain unclear. Thus, we explored the characterization of dissolved organic matter in coal waste and its biogeochemical implications. Our results highlight coal waste as primary succession for bacterial communities and provide new insights into the bacterial community assembly response to dissolved organic matter and geochemical properties at such sites. Biogeochemical parameters including pH, EC, nutrients, particle size, and enzymatic activities were affected differently by organic components. The electrical conductivity (EC) decreased after decades of natural weathering, whereas pH, moisture, available N and P, urease, phosphatase, soluble microbial byproduct‐like material, humic acid‐like organics, bacterial diversity, and richness increased significantly. Further taxonomical and co‐occurrence network analyses indicated that the primary succession of bacterial communities was strongly influenced by biogenically formed organic components, particle size, and available nutrients. Our results revealed that the humic‐like components facilitated the improved biogeochemical properties and succession of composition and structure of the bacterial community. It demonstrated that weathering‐induced organic matter degradation trigger biogeochemical property improvement and bacterial community succession, and further facilitate the transformation of coal waste into a plant growth substrate. Our results provide a reference for engineering and application practice of utilization and in‐situ remediation ecological reconstruction in coal waste deposits.
Elemental content allometries and silicon uptake rates of planktonic Rhizaria: Insights into their ecology and role in biogeochemical cycles
Authors: Laget Manon , Llopis‐Monferrer Natalia , Maguer Jean‐François , Leynaert Aude , Biard Tristan 
Published: December 2022

AbstractThe last two decades have shown the importance of Rhizaria in the biogeochemical cycles of carbon and silicon in modern oceans. This eukaryotic supergroup, which includes Radiolaria and Phaeodaria, represents an important part of zooplanktonic carbon biomass and contributes to carbon and silica export. Still, accurate estimations of their carbon biomass are hindered by poor knowledge of their elemental composition, contrasting with well‐established allometric carbon‐to‐volume relationships for smaller protists such as phytoplankton. Here, we directly measured carbon, nitrogen, and biogenic silica content as well as silicon uptake rates of planktonic Rhizaria. We highlight that size can be used as a predictor of elemental content for a broad variety of planktonic Rhizaria ranging from 200 μm to several mm, whereas size is weakly correlated with silicon uptake rates. Our results indicate that the scaling exponent of the carbon‐to‐volume allometry is significantly lower than those for smaller protists, underlining the low carbon strategy of these organisms. Still, we show that carbon and nitrogen densities span over four orders of magnitude, possibly accounting for the differences in depth ranges, nutritional modes and colonial or solitary forms. We estimate Rhizaria sinking speeds by combining carbon, nitrogen, and silica content data and show that great variability exists among the different taxa. Besides giving a better understanding of rhizarian ecology and biogeochemistry, these analyses, at the individual scale, are a first step to subsequent biomass and flux estimations at larger scales.
High Mechanical Strength and Multifunctional Microphase‐Separated Supramolecular Hydrogels Fabricated by Liquid‐Crystalline Block Copolymer
Authors: Liu Yang , Chen Lv , Yang Yuxuan , Chen Hongxiang , Zhang Xiongzhi , Liu Simin 
Published: December 2022

AbstractThe development of multifunctional supramolecular hydrogels with high mechanical strength and multifunction is in high demand. In this work, the diblock copolymer poly(acrylamide‐co‐1‐benzyl‐3‐vinylimidazolium bromide)‐block‐polyAzobenzene is synthesized through reversible addition−fragmentation chain transfer polymerization. The dynamic host–guest interactions between the host molecule cucurbit[8] uril and guest units are used to fabricate a 3D network of supramolecular hydrogels. Investigations on the properties of the supramolecular hydrogels show that the tensile stress of the sample is 1.46 MPa, eight times higher than that of hydrogel without liquid‐crystalline block copolymer, and the self‐healing efficiency of the supramolecular hydrogels at room temperature is 88.3% (fracture stress) and 100% (fracture strain) after 24 h. Results show that microphase‐separated structure plays a key role in the high‐strength hydrogel, whereas the host–guest interaction endows the hydrogel with self‐healing properties. The supramolecular hydrogels with high mechanical strength, photo‐responsivity, injectability, and biocompatibility can be used in various potential applications.
Single‐cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa
Authors: Hu Xueli , Wu Mingbo , Ma Teng , Zhang Yige , Zou Chaoyu , Wang Ruihuan , Zhang Yongxin , Ren Yuan , Li Qianqian , Liu Huan , Li Heyue , Wang Taolin , Sun Xiaolong , Yang Yang , Tang Miao , Li Xuefeng , Li Jing , Gao Xiang , Li Taiwen , Zhou Xikun 
Published: December 2022

AbstractKnowledge of the changes in the immune microenvironment during pulmonary bacterial acute and chronic infections is limited. The dissection of immune system may provide a basis for effective therapeutic strategies against bacterial infection. Here, we describe a single immune cell atlas of mouse lungs after acute and chronic Pseudomonas aeruginosa infection using single‐cell transcriptomics, multiplex immunohistochemistry, and flow cytometry. Our single‐cell transcriptomic analysis revealed large‐scale comprehensive changes in immune cell composition and high variation in cell–cell interactions after acute and chronic P. aeruginosa infection. Bacterial infection reprograms the genetic architecture of immune cell populations. We identified specific immune cell types, including Cxcl2+ B cells and interstitial macrophages, in response to acute and chronic infection, such as their proportions, distribution, and functional status. Importantly, the patterns of immune cell response are drastically different between acute and chronic infection models. The distinct molecular signatures highlight the importance of the highly dynamic cell–cell interaction process in different pathological conditions, which has not been completely revealed previously. These findings provide a comprehensive and unbiased immune cellular landscape for respiratory pathogenesis in mice, enabling further understanding of immunologic mechanisms in infection and inflammatory diseases.
Posttraumatic stress symptoms and chronic pain: Cross‐sectional associations with perception of sensory and social stimuli
Authors: Pierce Jennifer , Mohan Nitesh , Fatabhoy Megha G. , McAfee Jenna , Zhu Guohao , Hassett Afton L. 
Published: December 2022

ABSTRACTChronic pain and posttraumatic stress symptoms frequently co‐occur. Both may be associated with how individuals perceive stimuli; yet, to our knowledge, studies have not considered their joint effects on the perception of stimuli. In the present study, we examined main and interactive effects of chronic pain phenotype (no chronic pain; <4 regions of chronic pain; ≥4 regions of chronic pain) and high posttraumatic stress symptoms (>3 symptoms) on sensory sensitivity (i.e., external sensory sensitivity and somatic awareness) and sensitivity to social stimuli (i.e., hostility, perceived rejection, and rejection sensitivity). Adult patients with a self‐reported history of trauma or abuse (n = 305) completed across‐sectional, online survey. A chronic pain phenotype indicating ≥4 regions of chronic pain exhibited significant main effects on somatic awareness and hostility. High posttraumatic stress symptoms exhibited significant main effects on all sensory and social variables. An interaction between chronic pain phenotype and high posttraumatic stress symptoms was also found for perceived rejection, such that the association between high posttraumatic stress symptoms and higher perceived rejection was buffered for individuals with <4 regions of chronic pain. The findings suggest that both chronic pain phenotype, particularly ≥4 regions of chronic pain, and high posttraumatic stress symptoms are related to the perception of sensory and social stimuli. These findings may be important for understanding the impact of each condition on functioning and well‐being, as well as how to appropriately treat and manage symptoms.
Technical note: A knowledge graph approach to registering tumour specific data of patient‐candidates for proton therapy in the Netherlands
Authors: Kalendralis Petros , Sloep Matthijs , Choudhury Ananya , Seyben Lerau , Snel Jasper , George Nibin Moni , Veugen Joeri , Veening Martijn , Langendijk Johannes A. , Dekker Andre , van Soest Johan , Fijten Rianne 
Published: December 2022

AbstractThe registration of multi‐source radiation oncology data is a time‐consuming and labour‐intensive procedure. The standardisation of data collection offers the possibility for the acquisition of quality data for research and clinical purposes. With this study, we present an overview of the different tumour group data lists in the Dutch national proton therapy registry. Furthermore, as a representative example of the workings of these different tumour‐specific knowledge graphs, we present the FAIR (Findable, Accessible, Interoperable, Reusable) data principles‐compliant knowledge graph approach describing the head and neck tumour variables using radiotherapy domain ontologies and semantic web technologies. Our goal is to provide the radiotherapy community with a flexible and interoperable data model for data exchange between centres. We highlight data variables that are needed for models used in the model‐based approach (MBA), which ensures a fair selection of patients that will benefit most from proton therapy.
Technical note: Water‐equivalent thickness of Superflab bolus material at diagnostic x‐ray energies
Authors: Blunt Kayla F. , Marshall Emily L. , Guo Chao , Lu Zheng Feng , Reiser Ingrid 
Published: December 2022

AbstractPurposeThe purpose of this work was to determine the water‐equivalent thickness of Superflab bolus material for narrow and broad field‐of‐view (FOV) x‐ray geometries at diagnostic x‐ray energies.MethodsTransmission measurements were performed for incremental thicknesses of Superflab bolus material and water in narrow and broad FOV x‐ray geometries. The transmission data was fit to a non‐linear model for x‐ray transmission ‐ the Archer model. Water‐equivalent thickness of Superflab was calculated based upon fitting parameters to transmission curves for 75, 95, and 115 kV x‐ray tube voltages. Measured x‐ray transmission factors for water and Superflab were used to determine the water equivalence of Superflab.ResultsFor all x‐ray tube voltages and geometries, the water equivalence of Superflab was greater than one, indicating that Superflab is more attenuating than water. This effect was stronger for broad FOV geometries. At 95 kV, 30 cm of Superflab corresponded to 32.0 cm of water in the narrow FOV geometry, and 34.3 cm of water in the broad FOV geometry. The Archer model fitting parameters and Superflab water equivalence are reported for all x‐ray beam conditions explored in this work.ConclusionsSuperflab bolus material is more attenuating than water at diagnostic x‐ray energies. The Archer model and its respective fitting parameters reported in this work may be used to estimate the water‐equivalent thickness of Superflab for diagnostic x‐ray spectra.
Nurses' sense of organizational support, Self‐esteem and perceived professional benefits: A mediating model
Authors: Wang Mingjing , Wang Limin , Lu Caihong 
Published: December 2022

AbstractAimTo explore the mediating role of self‐esteem in the relationship between perceived organizational support and professional benefits among Registered Nurses in China.DesignThis was an online, cross‐sectional study involving 1850 nurses from six hospitals in China.MethodsData were collected using a 4‐part questionnaire including a sociodemographic questionnaire, perceived organizational support scale, self‐esteem scale and brief nurses' perceived professional benefits questionnaire from September to November 2021. Structural equation modelling (SEM) was used to explore the associations among them.ResultsPerceived organizational support was positively correlated with self‐esteem and perceived professional benefits among nurses, whereas self‐esteem positively predicted nurses' perceived professional benefits . Self‐esteem partially mediated the relationship between the two variables. The ratio of the mediating effect to the total effect was 16.7%.
Informing the design of urban green and blue spaces through an understanding of Europeans' usage and preferences
Authors: Jakstis Kristen , Dubovik Maria , Laikari Arto , Mustajärvi Kaisa , Wendling Laura , Fischer Leonie K. 
Published: December 2022

Abstract

In light of global climate change and the biodiversity crisis, making cities more resilient through an adjusted design of urban green and blue spaces is crucial. Nature‐based solutions help address these challenges while providing opportunities for nature experiences, and providing cultural ecosystem services that support public health. The COVID‐19 pandemic and its associated stressors highlighted the interrelated socio‐ecological services provided by nature‐based solutions like urban green and blue spaces.

This pan‐European study therefore aimed to enhance the socio‐ecological understanding of green and blue spaces to support their design and management. Using an online survey, green and blue space preferences, usage, and pandemic‐related changes in greenspace visit and outdoor recreation frequencies were examined.

Greenspace visit and outdoor recreation frequencies were associated with respondents’ (N = 584 from 15 countries) geographical location, dominant type of neighbourhood greenspace and greenspace availability during the pandemic, but not greenspace perceptions or sociodemographic background.

Greenspace visit and outdoor recreation frequencies were generally high; however, Southern Europeans reported lower greenspace visit and outdoor recreation frequencies both before and during the pandemic than Northern Europeans. Many Southern Europeans also reported having few neighbourhood greenspaces and low greenspace availability during the pandemic.

The most common outdoor recreational activity among respondents before the pandemic was walking or running with the most frequently stated purpose of time spent outdoors being restorative in nature (i.e. relaxing or calming down). Most Europeans had positive perceptions of green and blue spaces with preferences for structurally diverse and natural or unmanaged green elements.

This highlights the importance of accessible green and blue spaces both in everyday life and during times of crisis. Stakeholders, their preferences, and regional and cultural differences should be included in the co‐design of urban green and blue spaces to maximize their potential for both people and nature.

Read the free Plain Language Summary for this article on the Journal blog.
Effect of heat input on temperature characteristics and fusion behavior at bonding interface of CFRTP induction welded joint with carbon fiber susceptor
Authors: Wang Feiyun , Zhang Pingze , Luo Jiebang , Li Binbin , Liu Gang , Zhan Xiaohong 
Published: December 2022

AbstractInduction welding is a suitable and promising technique for the assembly of thermoplastic composite structural components. In this work, the simulation and experimental research of induction welding devoted to the quality and efficient bonding of carbon fiber reinforced polyetheretherketone (CF/PEEK) laminates utilizing carbon fiber susceptors is presented. The carbon fiber susceptors that consist of carbon fabric and resin film same as base materials are prepared as heating elements to concentrate the heat generation on the welding interface. A transient three‐dimensional finite element model is developed to analyze the heating characteristics and temperature distribution during the welding process. Induction welding experiments are carried out to investigate the effect of heat input on fusion behavior at the bonding interface, focusing on macro appearances, interfacial fusion morphologies and welding defects. The results show that heat distribution at the welding interface is uneven, presenting that the temperature at the edge is higher than at the center. An increase in heat input facilitates the fusion of the joints, which is demonstrated by a smaller relative width of the fusion zone and expander effective bonding area. However, macro and micro welding defects start to appear in joints due to the massive melting, ablation and thermal decomposition of resin, which are caused by excessive heat input. The micro defects dominated by pores are divided into four types according to their positions, sizes, morphologies and causes of formation.
Antibacterial and electrochemical evaluation of electrospun polyethersulfone/silver composites as highly persistent nanomaterials
Authors: Hernández‐Orozco María Mónica , Castellanos‐Espinoza Raúl , Hernández‐Santos Nuri Aranzazú , Ramírez‐Montiel Fátima Berenice , Álvarez‐Contreras Lorena , Arellano‐Arreola Víctor Manuel , Padilla‐Vaca Felipe , Arjona Noé , España‐Sánchez Beatriz Liliana 
Published: December 2022

AbstractSilver‐based antibacterial nanoparticles have demonstrated indices of possible bacterial resistance after consecutive periods of time, limiting their application on antibacterial surfaces. In this work, we fabricate electrospun composites of polyethersulfone (PES) and silver nanoparticles (AgNPs), displaying highly‐persistent antibacterial behavior after 10 bacterial expositions. The herein‐reported membranes were synthesized by changing the Ag loading (0.05, 0.5, and 5 wt%), adding polyvinylpyrrolidone as a dispersion promoter, and the resulting composites were evaluated after a different number of bacterial expositions against Escherichia coli (E. coli) aiming to determine their bacterial tolerance. SEM micrographs revealed that the PES fiber diameters decreased as a function of the silver content from 798 to 398 nm, attributed to the conductivity and the viscosity improvement by AgNPs introduction in the polymer solution during the electrospun process. Moreover, the surface roughness (determined by atomic force microscopy) and the contact angle changed with the Ag content, increasing the hydrophilic behavior and promoting a better interaction with the bacteria suspended in the solution. Antibacterial activity kinetics of PES/AgNPs fibers showed 75% bacterial inhibition against E. coli in the composite with 0.5 wt% Ag after 3 h of exposition, and 75% after 1.5 h in composites with 5 wt% of Ag. The ca. 25% survival bacteria were used for consecutive bacterial tolerance assays, where the antibacterial behavior was constant after 10 expositions. Interestingly, electrochemical tests confirmed that PES fibers could control the silver‐releasing process. Thus, PES/AgNPs composites avoid the bacterial adaptation process, which makes them suitable as functional antimicrobial nanomaterials.
SVECV‐f12: A composite scheme for accurate and cost‐effective evaluation of reaction barriers. II. Benchmarking using Karton's BH28 barrier heights database
Authors: Kieninger Martina , Ventura Oscar N. 
Published: December 2022

AbstractA simple composite scheme developed for the calculation of accurate reaction barriers, and benchmarked against small systems, is applied here to compute barriers in the BH28 data set published in 2019 by Karton. This set comprises more complex transition states, with up to 7 non‐hydrogen and 10 hydrogen atoms, which barriers calculated at the SVECV‐f12 level are compared to the accurate CCSDT(Q)/CBS values obtained by Karton. A mean absolute difference of 2.4 kJ mol−1 and a root mean square difference of 1.9 kJ mol−1 were obtained, underlying the chemical accuracy of the protocol.