bluuebunny/crossref_metadata_2025_split huggingface dataset:
Period 3 regulates mitochondrial function via the NAD+-SIRT3 axis in mice
Authors: XIE Xiaoxian , Shu Ruonan , Zhang Mengya , Cui Donghong , Sun Lei , Li Zezhi , Han Qi 
Published: July 2022

Abstract
Mitochondrial fission-fusion dynamics and bioenergetics are strongly clock-controlled. However, the mechanistic relationship between the clock and mitochondrial function remains largely unknown. Here we revealed that the clock gene Per3 plays an essential role in the maintenance of mitochondrial homeostasis. Both in vitro and in vivo deletion of Per3 consistently led to the disrupted mitochondrial integrity, including decreased mitochondrial membrane potential, impaired fission-fusion dynamics, increased oxidative stress, and altered mitochondrial bioenergetics. Mechanistically, we showed the binding of PER3 and BMAL1 to E-box regulatory elements in the promoter region of the nicotinamide phosphoribosyltransferase (NAMPT) gene promoted the gene activation as well as increased the NAMPT-dependent NAD+ generation, which in turn induced the enzymatic activity of mitochondrial deacetylase SIRT3. Interestingly, administering nicotinamide mononucleotide (NAM), an NAD+ precursor, protected cells from the Per3 deletion-induced NAD+ decline and associated mitochondrial dysfunction, whereas blocking of SIRT3’s acetylation activity with 3-(1H-1, 2, 3-triazol-4-yl) pyridine abolished this beneficial effect of NAM on the global mitochondrial protein acetylation and homeostasis mechanisms. Collectively, we demonstrate that Per3 maintains mitochondrial integrity by regulating NAD+-dependent SIRT3 activity and highlight a critical role for PER3 in mitochondrial homeostatic function.
Noncovalent interactions between benzochalcogenadiazoles and nitrogen bases
Authors: Zhang Lili , Zeng Yanli , Li Xiaoyan , Zhang Xueying 
Published: July 2022

Abstract
A theoretical study has been carried out on the intermolecular interactions between tetrafluoro-benzochalcogenadiazoles (chalcogen = S, Se, Te) and a series of nitrogen bases (FCN, ClCN, NP, trans-N2H2, pyridine, pyrazole, imidazole) at the B97-D3/def2-TZVP level, to obtain a better insight into the nature and strength of Ch···N chalcogen bond and secondary interaction in the binary and 1:2 ternary complexes. The dispersion force plays a prominent role on the stability of the sulphur complexes, and the electrostatic effect enhanced for the heaver chalcogen complexes. Most of intermolecular bonds display the characters of closed-shell and noncovalent interaction. For the complexes involving pyridine and imidazole, chalcogen bond is stronger than hydrogen bond, while the strength of chalcogen bond is equivalent to the secondary interaction for other complexes. With the addition of nitrogen base in the 1:2 complexes, chalcogen bond is weakened, while the secondary interaction remains unchanged. In the 1:2 complexes formed by pyridine and imidazole, stronger chalcogen bond results in larger negative cooperativity than that of other complexes.
Magnitude of COVID-19 vaccination among Ethiopian public transport drivers; Cross- sectional study
Authors: Ergena Asrat Elias , Tesfaye Amensisa Hailu , Kasahun Asmamaw Emagn , Sima Faisel Dulla , Mekonnen Gizework Alemnew , Simegn Wudneh 
Published: July 2022

Abstract
Background
In Ethiopia, the approved vaccines for Corona-Virus disease (COVID-19) are being distributed to the population by their order of risk and exposure due to the limited number of available vaccines. Public transport workers are among those who are labeled as a high-risk population and on the frontlines of getting the COVID-19 vaccination. So this study was conducted to assess the magnitude of the COVID-19 vaccine intake among public transport drivers.
Methods
A community-based cross-sectional study was conducted among 384 participants in Gondar, Ethiopia from October 1, 2021 to November 15, 2021. The data was collected using self-administered questionnaires and analyzed. P-value ≤ 0.05 in a multivariable logistic regression model was considered statistically significant.
Results
Among the 384 study participants, 196 (51%) of them received the COVID-19 vaccine. Among those who were not vaccinated, 95 (50%) of the participants did not take the vaccines due to fear of the adverse effects of the vaccines. In multivariate logistic regression analysis, being affiliated with the Islamic religion, having a health professional family member or friend, and having a history of sign/symptoms of COVID-19 in the study participants were found to be associated with the vaccination status against COVID-19.
Conclusion
The overall COVID-19 vaccine intake by the study participants was low. To enhance the magnitude of the vaccination of this high-risk population, governmental organizations and other stakeholders should focus on increasing awareness in order to increase the willingness of the population.
A novel model-based identification and validation of pivotal RNA-binding protein in the evolution of glioma
Authors: Liu Xudong , Li Rui , Liu Zhe , Wang Yafan , Zhang Yu , Fan Mengdi , Ma Ying , Peng Tao , Zhao Lijun , Fan Jinke , Bai Jiangshan , Song Xinqiang , Zhang Yating , Wang Lei 
Published: July 2022

Abstract
Background
Glioma is a primary malignant cancer of the most common and aggressive sort occurring in the neuroectoderm, with an extremely high incidence of morbidity, mortality, and recurrence. There are limited treatment options for glioma. It is recognized that dysregulation of RNA binding proteins (RBPs) is intimately involved in the occurrence and progression of multiple malignant cancers. However, the role of RBP in glioma is not fully understood. In the current study, we used a new model to predict the course of glioma development and prognosis related to RBP.
Methods
To begin with, we obtained RNA-seq profiles of glioma and matched clinical data from the Chinese Glioma Genome Data (CGGA) database. The unsupervised clustering algorithms such as the K-means clustering algorithm, t-SNE, U-MAP, and principal component analysis were used to identify tumor subtypes that were pivotal in the development of glioma. Subsequently the progression of tumor subtypes was determined by the MS scoring model and proposed time series analysis. Next, RBPs with a major role in the progression of glioma were identified by weighted gene co-expression network analysis and Lasso Cox regression models. Functional analysis of key RBP-related genes was performed by gene set enrichment analysis (GSEA) and the STRING database to reveal the potential mechanisms of the biomarker.
Results
A total of six tumor subgroups were identified and were strongly homogeneous among subgroups. The stages of progression of the six tumor subgroups were identified by the proposed time-series analysis and MS model. We confirmed that BCLAF1 was correlated with survival in glioma patients and was closely associated with the progression of glioma by weighted gene co-expression network analysis, Lasso Cox, and multivariate Cox regression analysis. Finally, Functional annotation shows that BCLAF1 may influence the progression of glioma through RNA shearing that affects cell cycle, Wnt signaling pathway, and other cancer development signals.
Conclusions
In summary, the present study first identified six subtypes of glioma progression as well as the malignancy ranking. Secondly, it was established that BCLAF1 could be used as an RBP-related prognostic marker with extremely important applications in the clinical diagnosis and personalized treatment of glioma.
Early reduction of cerebral lactate in Alzheimer’s disease mouse model: The relationship with Aβ production
Authors: Zhang Mao , Wang Yanyan , Xu Xiaofeng , Dai Limeng , Chen Xuedan , Wang Kai , Bai Yun , Guo Hong 
Published: July 2022

Abstract
Lactate arouses an increasing concern in recent studies of Alzheimer’s disease (AD). At late stage of AD, amyloid beta (Aβ) directly promotes lactate level, continuously activating microglial inflammation and aggravating neuronal damage. However, whether lactate fluctuation can effect on Aβ hasn’t been recognized. Especially, it is observed a reduction of lactate in the hippocampus and cortex of early AD mouse model. Hence, this study discusses the relation between decreased lactate and Aβ production at early stage of AD, and its possibly mechanism. As results showed, a specific receptor of lactate, G-protein-coupled receptor 81 (GPR81) and its downstream of phosphatidylinositol 3 kinase /protein kinase B (PI3K/Akt)-cyclic-adenosine-monophosphate response element-binding protein (CREB) is suppressed in the cortex and hippocampus of early AD mouse model. Moreover, deoxyribonucleic acid methyltransferase 1 (DNMT1) is reduced in the presence of increased beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) and Aβ. Previously, it is observed that PI3K/Akt-CREB inhibition leads to DNMT1 downregulation. As an indispensable methyltransferase, DNMT1 reduction can directly inhibit the methylation of specific loci within BACE1 gene promoter, leading to increased BACE1 and Aβ production. Hence, this study proposes a novel insight that lactate reduction contributes to Aβ production at early period of AD. The underlying mechanism is that reduced lactate can hardly activate GPR81-PI3K/Akt-CREB, which results in DNMT1 reduction, and then increasing BACE1 level and Aβ production (Graphic abstract).
Coherence interpretations of nonlocal quantum correlation based on a quantum eraser
Authors: Ham Byoung S. 
Published: July 2022

Abstract
Bell inequality violation is a quantitative measurement tool of quantum entanglement between space-like separated particles. Quantum entanglement is the heart of quantum information science, in which the resulting nonlocal correlation between the paired particles is a unique property of quantum mechanics. Over the last few decades, intensive research has been conducted to understand nonlocal quantum features based on the particle nature of quantum mechanics. Here, the role of coincidence detection is analyzed in a simple interferometer using a quantum eraser. To understand the nonlocal quantum feature, coincidence detection between two output photons of an interferometer is coherently analyzed for Bell inequality violations. Based on this understanding, a classical model of the nonlocal quantum correlation is finally presented using coherent light via wave mixing and heterodyne detection.
Serum miR-4488 as a Potential Biomarker of Lean Nonalcoholic Fatty Liver Disease
Authors: Shen Nan , Tang Ling , Qian Yufan , Pan Jielu , Pan Jiashu , Miao Hongyu , Zhang Haiyan , Fang Hong , Yu Xiao , Xing Lianjun 
Published: July 2022

Abstract
In lean individuals, NAFLD is not a benign disease,and these patients have longterm morbidity and mortality similar to those of their nonlean counterparts.It is urgent to find biomarkers for noninvasive and early detection. miRNAs are useful biomarkers for detection.The aims of this study were to investigate the potential role of serum miRNAs in the detection of lean NAFLD and to explore the possible pathogenesis of lean NAFLD.Here, a total of 498 patients with NAFLD and 98 healthy controls were included to compare the clinical characteristics of lean NAFLD patients(BMI < 23 kg/m2),nonlean NAFLD patients(BMI ≥ 23kg/m2) and normal healthy individuals.A total of fourteen serum samples were collected for high-throughput profiling to identify altered miRNA expression patterns in lean NAFLD.The candidate miRNA miR-4488 was identified by filtering based on studies in a second independent cohort(31LNs,62NLNs and 72HIs) that included qRT-PCR analysis.GO enrichment, KEGG enrichment, and PPI network analyses were performed to investigate the potential molecular mechanism of miR-4488 in lean NAFLD. miR-4488 was selected as the candidate biomarker for lean NAFLD. The serum level of miR-4488 was increased in LNs compared to HIs(P < 0.0001) and to NLNs(P = 0.025).miR-4488 had acceptable performance in predicting[area under the curve(AUC) = 0.794,0.698]lean NAFLD. Moreover,GO and KEGG enrichment analyses revealed that the differentially expressed target genes were mainly involved in choline metabolism in cancer, the TNF signaling pathway and the p53 signaling pathway.PPI analysis identified ARHGAP1, SLC10A1 and SIX5 as the hub genes.Taken together, our findings indicate that serum miR-4488 is a potential biomarker for diagnosing and predicting the pathogenetic mechanisms of lean NAFLD.
Stochastic Modeling of Microstrip Coupled Line Bandpass Filter
Authors: Wafa Mohamed , El-Batawy Yasser , Dakroury Sameh 
Published: July 2022

Abstract
Coupled Line Microstrip Bandpass Filters are considered as a promising candidate for bandpass filter for wireless and high frequency applications because of their small size, low cost and easy engineered fabrication. In this paper, a stochastic analysis of this coupled line bandpass filter which is based on Monte Carlo Model is developed, where the tolerances in the design parameters of this microstrip bandpass filter and their impacts on the filter performance are investigated. These design parameters include the thickness of the strips of the bandpass filter, their lengths and the spacing between them. The mean, the standard deviations and the probability distribution function of both the central frequency and the operating bandwidth are studied.
Exploring Quaternion Flipping of the Sun’s Magnetic Field in the Centennial Sunspot Record
Authors: Baker Robert G.V. 
Published: July 2022

Abstract
Sunspots are a major proxy for measuring the solar input into the Earth’s atmospheric dynamics. This paper explores whether sunspot evolution is underpinned by quaternion flipping in the 19th and 20th century sunspot histories. Quaternion flipping occurs when there is self-similarity in an R-matrix of sunspot cycles, where successive cycles evolve by rotating 1800 in a plane and the subsequent conjugation of subsequent cycles is in the reverse order. The historical sunspot record is partitioned according to quadratic clusters and significant periodicities in the spectral analysis, such as the 65.8 year signal. The flipping is then simulated by reversing the time axis between two adjoining ~ 66 year cycles in the sunspot record, producing an R3R2=-R4 quaternion flip, namely, between 1822–1888 and a reversed time series of 1976 − 1910. The Hausdorff dimensions and multi-taper spectral results for each flipped period are calculated in their entirety, where there was a 94.2% common spectral signal and 98.1% self-similarity. The R3R2=-R4 flip maintained a Hausdorff dimension of between 1.080 ± 0.025. The Hausdorff dimension of 1.081 is significant, since it generates a quadratic Julia set envelope of z2+c where c = ¼. For any values greater than 1.081, the Julia magnetic loops collapse and the quadratic point at z = ½ bifurcates into a pair of repelling fixed points off the real axis (that is, magnetic dipoles). This may explain the flipping in polarity after sunspot maximums and the self-similarity in frequencies between sunspot numbers and solar emissions. Further, there is a possibility that this centennial flipping can be traced from the Little Ice Age and the next phase is a centennial maximum mid-century. This research shows a fundamental relationship in a developing ‘fractal mechanics’ of solar emissions and the nature of its periodic input into the Earth’s atmospheric dynamics.
Phragmites australis dieback in the Mississippi River Delta: Chemical profiles of healthy, dieback and dredge soils and impacts on plant biomass
Authors: Lee Herie , Diaz Rodrigo , Cronin James T. 
Published: July 2022

Abstract
Phragmites australis is exhibiting extensive dieback in the Lower Mississippi River Delta (MRD). We explored the potential for restoration of these marshes by 1) characterizing the chemical profiles of soils collected from healthy and dieback stands of P. australis and from sites recently created from dredge-disposal soils that were expected to be colonized by P. australis and 2) experimentally testing the effects of these soil types on the growth of three common P. australis lineages, Delta, Gulf and European. Soil chemical properties included Al, Ca, Cu, Fe, K, Mg, Mn, Na, P, S, Zn, % organic matter, % carbon, % nitrogen, and pH. Dieback soils were characterized by higher % organic matter, % carbon, % nitrogen, and higher S and Fe concentrations, whereas healthy soils had higher Cu, Al, P and Zn. In comparison, dredge sites were low in nutrients and organic matter compared to healthy soils. Rhizomes of each P. australis lineage were planted in each soil type in a common garden and greenhouse and allowed to grow for five months. Aboveground biomass was 16–23% lower in dieback and 44% lower in dredge soils than in healthy soils. Dieback soils most negatively affected Delta followed by European and Gulf lineages. Although dredge and dieback sites are not optimal for P. australis growth, plants can thrive on these soils, and we recommend restorative measures be initiated as soon as possible to minimize soil erosion.
Clinical Features, Hospitalisation and Deaths associated with Monkeypox: A systematic review and meta-analysis
Authors: Benites-Zapata Vicente , Ulloque-Badaracco Juan R. , Alarcon-Braga Esteban A. , Hernandez-Bustamante Enrique A. , Mosquera-Rojas Melany D. , Bonilla-Aldana D. Katterine , Rodriguez-Morales Alfonso J. 
Published: July 2022

Abstract
Introduction: A multicountry monkeypox disease (MPX) outbreak began in May 2022 in Europe, leading to the assessment as a potential Public Health Emergency of International Concern (PHEIC) on June 23, 2022. Some observational studies have partially characterised clinical features, hospitalisations, and deaths. However, no systematic reviews of this MPX outbreak have been published.Methods: We performed a systematic review with meta-analysis, using five databases to assess clinical features, hospitalisations, complications and deaths of MPX confirmed or probable cases. Observational studies, case reports and case series, were included. We performed a random-effects model meta-analysis to calculate the pooled prevalence and 95% confidence interval (95%CI). In addition, we carried out a subgroup analysis according to the continents and a sensitivity analysis excluding studies classified as having a high risk of bias.Results: A total of 19 articles were included, using only 12 articles in the quantitative synthesis (meta-analysis). For 1958 patients, rash (93%, 95%CI 80-100%), fever (72%, 95%CI 30-99%), pruritus (65%, 95%CI 47-81%), and lymphadenopathy (62%, 47-76%), were the most prevalent manifestations. Among the patients, 35% (95%CI 14-59%) were hospitalised. Some 4% (95%CI 1-9%) of hospitalised patients had fatal outcomes (case fatality rate, CFR).Conclusion: MPX is spreading rapidly, with a third of hospitalised patients, but less than 5% with fatal outcomes. As this zoonotic virus spreads globally, countries must urgently prepare human resources, infrastructure and facilities to treat patients according to the emerging guidelines and the most reliable clinical information.
Effects of combined test-dose and therapeutic drug monitoring strategy in exposure-directed busulfan
Authors: Iemura Tomoki , Kondo Tadakazu , Ueda Atsushi , Maeda Takeshi , Kitawaki Toshio , Arai Yasuyuki , Kanda Junya , Ikeda Takashi , Imada Kazunori , Ishikawa Takayuki , Anzai Naoyuki , Itoh Mitsuru , Takeoka Tomoharu , Akasaka Takashi , Yago Kazuhiro , Yonezawa Akihito , Arima Nobuyoshi , Kitano Toshiyuki , Nohgawa Masaharu , Watanabe Mitsumasa , Moriguchi Toshinori , Yamashita Kouhei , Ueda Yasunori , Matsumoto Kana , Takaori-Kondo Akifumi 
Published: July 2022

Abstract
Although exposure-directed busulfan (BU) dosing can improve allogeneic hematopoietic stem cell transplantation outcomes, there is still large variability in BU exposure with test-dose alone due to BU clearance change caused by drug interactions. We conducted a single-arm Phase II trial (UMIN000014077) using the combined test-dose and therapeutic drug monitoring strategy (PK-guided group) and compared the outcomes with an historical cohort receiving a fixed-dose (fixed-dose group). The first eight and second eight doses were adjusted based on the area under the blood concentration-time curve (AUC) of the test and first doses, respectively. The BU clearance at the first dose decreased in more patients receiving fludarabine (FLU) than those receiving BU and cyclophosphamide (BU/CY) from the test dose. The simulated total AUC with test-dose only was significantly higher in patients receiving FLU than in those receiving BU/CY, but the simulated AUC with the combined strategy was comparable. The 100-day progression-free survival in PK-guided group was not inferior to that in fixed-dose group. For the FLU-containing regimens, the PK-guided group showed decreased relapse, and favorable overall survival at one year. The combined strategy effectively controlled the BU exposure close to the target levels, potentially improving efficacy, especially in patients receiving the FLU-containing regimen. Clinical Trial Registry #UMIN000014077
Multidimensional Poverty Analysis in Coastal Region of Sindh Province, Pakistan; a Case Study of Thatta and Badin District
Authors: Dahri Ghulam Nabi , Talpur Basit Ali , Wang Jing , Hu Linyue , Khoso Shumaila Tabassum 
Published: July 2022

Abstract
Multidimensional measures provide an alternative lens through which poverty may be viewed and understood deeply. It is argued that monetary variables (such as income or expenditure) are unable to truly evaluate human well-being (Idrees and Baig, 2017). It is an emerging phenomenon in the South Asian countries where 49% of people are multidimensional poor. The dimension wise breakdown shows the cooking fuel, flooring, nutrition, electricity, child mortality and schooling have major contributors among overall multidimensional poverty (Dhruba et al., 2015). Across 107 developing countries, 1.3 billion people are suffering from acute income poverty with a certain disparity in magnitude. While the dilemma of poverty has become a leading challenge in the history of the developing world, due to its extensive impact on the developmental process (UNDP, 2020). Analysis of Multidimensional poverty in the coastal region of Sindh; Thatta and Badin. To identify the impact of multidimensional poverty on socioeconomics conditions in the coastal region. To analyze the Multidimensional poverty in districts Thatta and Badin and examine the key factor influencing the multidimensional poverty in the study area. Primary data was collected to achieve targets and meet the objectives of the study. A random sampling technique used for the data collection procedure, total sample size of the respondents was 100. Data entered and arranged in a coding system, analyzed through SPSS software and MS Office. The Alkire and Foster method was used for the poverty analysis. We observed that Badin is more derivate as compare to the district Thatta in almost all indicator set by OPHI. Basic facilities needed for improvement through public private partnerships, daily earning source should be increase, and water availability should be improved specially in tail area of Badin district.