bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Macrophage Phenotype Rewired by Hantaan Virus Constrains the Magnitude of Inflammatory Responses in Murine versus Humans
Authors: zhang fanglin , Ma Hongwei , Yang Yongheng , Nie Tiejian , Yan Rong , Si Yue , Wei Jing , Li Mengyun , Liu He , Ye Wei , Zhang Hui , Cheng Linfeng , Zhang Liang , Lv Xin , Luo Limin , Xu Zhikai , Zhang Xijing , Lei Yingfeng 
Published: January 2022

Abstract
Hantaan virus (HTNV) is principally maintained and transmitted by rodents in nature, the infection of which is non-pathogenic in the field or laboratory mouse, but can cause hemorrhagic fever with renal syndrome (HFRS) in human beings, a severe systemic inflammatory disease with high mortality. It remains obscure how HTNV infection leads to disparate outcomes in distinct species. Here, we revealed a differential immune status in murine versus humans post HTNV infection, which was orchestrated by the macrophage reprogramming process and characterized by late-phase inactivation of NF-κB signaling. In HFRS patients, the immoderate and continuous activation of inflammatory monocyte/macrophage (M1) launched TNFα-centered cytokine storm and aggravated host immunopathologic injury, which can be life-threatening; however, in field or laboratory mice, the M1 activation and TNFα release were significantly suppressed at the late infection stage of HTNV, restricting excessive inflammation and blocking viral disease process, which also protected mice from secondary LPS challenge or polymicrobial sepsis. Mechanistically, we found that murine macrophage phenotype was dynamically manipulated by HTNV via the Notch-lncRNA-p65 axis. At the early stage of HTNV infection, the intracellular domain of Notch receptor (NICD) was activated by viral nucleocapsid (NP) stimulation and potentiated the NF-κB pathway by associating with and facilitating the interaction between IKKβ and p65. At the late stage, Notch signaling launched the expression of diverse murine-specific long non-coding RNAs (lncRNAs) and attenuated M1 polarization. Among them, lncRNA 30740.1 (termed as lnc-ip65, an inhibitor of p65) bound to p65 and hindered its phosphorylation, exerting negative feedback on the NF-κB pathway. Genetic ablation of lnc-ip65 shifted the balance of macrophage polarization from a pro-resolution to an inﬂammatory phenotype, leading to superabundant production of pro-inﬂammatory cytokines and increasing mice susceptibility to HTNV infection or bacterial sepsis. Collectively, our findings identify an immune braking function and mechanism for murine lncRNAs in inhibiting p65-mediated M1 activation, opening a novel therapeutic avenue of controlling the magnitude of immune responses for HFRS and other inflammatory diseases.
Flood and tides trigger longest measured sediment flow that accelerates for thousand kilometers into deep-sea
Authors: Talling Peter , Baker Megan , Pope Ed , Jacinto Ricardo Silva , Heijnen Maarten , Hage Sophie , Simmons Stephen , Hasenhündl Martin , Heerema Catharina , Ruffell Sean , McGhee Claire , Apprioual Ronan , Ferrant Anthony , Cartigny Matthieu , Parsons Daniel , Clare Michael , Tshimanga Raphael , Trigg Mark , Cula Costa , Faria Rui , Gaillot Arnaud , Bola Gode , Wallace Declan , Griffiths Allan , Nunny Robert , Urlaub Morelia , Peirce Christine , Burnett Richard , Neasham Jeffrey , Hilton Robert 
Published: January 2022

Abstract
Here we document for the first time how major rivers connect directly to the deep-sea, by analysing the longest runout sediment flows (of any type) yet measured in action. These seafloor turbidity currents originated from the Congo River-mouth, with one flow travelling >1,130 km whilst accelerating from 5.2 to 8.0 m/s. In one year, these turbidity currents eroded 1-2 km3 of sediment from just one submarine canyon, equivalent to 14-28% of the annual global-flux from rivers. It was known earthquakes trigger canyon-flushing flows. We show major river-floods also generate canyon-flushing flows, primed by rapid sediment-accumulation at the river-mouth, but triggered by spring tides weeks to months after the flood. This is also the first field-confirmation that turbidity currents which erode can self-accelerate, thereby travelling much further. These observations explain highly-efficient organic carbon transfer, and have important implications for hazards to seabed cables, or how terrestrial climate change impacts the deep-sea.
Clausena Harmandiana Root Extract Improved Amyloid-β Induced Cognitive Impairments in Rats By Reducing Aβ1-42 Protein Levels and Neuroinflammation
Authors: Nillert Nutchareeporn , Boonyarat Chantana , Welbat Jariya Umka , Bunreungthong Komsun , Puthongking Ploenthip , Pannangrong Wanassanun 
Published: January 2022

Abstract
Background: Neuroinflammation caused by amyloid‐β (Aβ) is associated with Alzheimer’s disease (AD) pathogenesis. In AD, Aβ accumulation can activate the surrounding microglia which followed by the synthesis and release of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNFα), results in cognitive impairments. Clausena harmandiana (CH) is an herb in the Rutaceae family and has been used in folk medicine for the treatment of illness such as stomachache and headache, and as a health tonic. It is interesting that CH root extract (CHRE) exhibits various anti-inflammatory and other pharmacological activities, but there has not been any study in Alzheimer's disease-like animal models.Objectives: This study aimed to investigate the effects of CHRE on Aβ1-42-induced cognitive impairments, increased Aβ1-42 protein levels and neuroinflammation. Methods: Forty-eight adult male Sprague-Dawley rats (250-300 g) were randomly divided into 6 groups (n=8). The rats were given 0.5% sodium carboxymethylcellulose, Celebrex® (10 mg/kg BW) or CHRE (125, 250, and 500 mg/kg BW) and not given any treatment by oral gavage for 35 days. On day 21, all treated rats were injected with aggregated Aβ1-42 at a concentration of 1 µg/µl into both lateral ventricles (1 µl/side), while untreated rats were injected with sterilized normal saline. Ten days later, their recognition memory was assessed using the novel object recognition test. At the end of the experiment, all rats were euthanized by an overdose of thiopental sodium (120 mg/kg BW) and transcardial perfusion with 0.9% normal saline solution, to observe Aβ1-42 protein levels and the expression of inflammatory markers (CD11b-positive microglia, IL-1β, and TNFα) in the cerebral cortex and hippocampus.Results: The results indicated that pretreatment with CHRE at all doses improved impairment of short- and long-term recognition memory. In addition, CHRE significantly decreased Aβ1-42 protein levels and the expression of inflammatory markers in both brain regions as well as pretreatment with Celebrex®.Conclusions: This suggests that CHRE has a potential therapeutic effect against Aβ1-42-induced cognitive impairments by reducing Aβ1-42 protein levels and neuroinflammation.
Designing a Smart Pillow for Sleep Quality Remote Monitoring
Authors: OLIVEIRA VINICIUS , Pereira Felisberto , Carvalho Nuno , Lopes Sérgio 
Published: January 2022

Abstract
This paper proposes a low cost IoT solution to detect head movements and positions of a patient by means of Force Sensing Resistors positioned on a pillow and connected to a micro-controller collecting patient data anytime, when sleeping, sending it to the cloud and making it available to healthcare professionals. The impact of this work is focused on monitoring sleep quality, using low-cost and easy to use pillows in an ambulatory scenario, without the need of expensive and dedicated sleeping rooms for sleep monitoring, which most of the times affect patient sleep and degrades the quality of the measurement.In this case it is possible to monitor the patient’s behavior throughout the entire sleep, important for detecting factors causing minor head and neck injuries and even checking for events of long pauses in respiratory rate.
Factors Influencing Social-Learning Experience in Nearby Pocket Parks On Campus Ground
Authors: Salih Sarah , Ismail Sumarni , Ismail Nor Atiah , Ujang Norsidah , Asif Nayeem 
Published: January 2022

Abstract
Nearby pockets on campus grounds have become necessary learning sustainable settings to improve the academic experience by promoting outdoor social and learning activities. However, many universities still focus mainly on formal indoor learning and lack outdoor education that meets modern academic outcomes. Therefore, the current study aimed to identify the factors affecting students' social-learning experience in nearby pocket parks on campus ground, focusing on the tropical regions. The current study employed a questionnaire survey conducted in three Malaysian universities to collect data from 408 participants. The results showed various types of influencing factors that affect the social-learning experience in nearby pockets on campus ground, including landscape elements and activities, environmental factors, and access to these spaces. The results also indicated that students' demographics, including gender, education status, and university, influenced the outdoor social-learning experience. The current study contributed information to the development of on-campus sustainable settings for integrating nearby pockets in social interaction and learning activities in order to improve the academic social-learning experience.
Effects of Air Pollutants on Mortality of Chronic Kidney Disease Patients with Green Spaces Exposure: A Large Observational Study
Authors: Jung Jiyun , Kim Yong Chul , Lee Hyewon , Shin Sung Joon , Kim Yon Su , Kim Sejoong , Lee Jung Pyo , Kim Ho , Park Jae Yoon 
Published: January 2022

Abstract
With increasing air pollution, the association between green space exposure and health outcomes is a global health concern. The relationship between air pollution and the survival of patients with chronic kidney disease (CKD) who are exposed to residential greenness is yet to be elucidated. This study aimed to determine this relationship in Seoul between 2002 and 2015. A time-varying survival analysis was conducted to investigate the association between long-term exposure to air pollutants and mortality in 29,602 patients with CKD living in residential environments with small and large green infrastructure. The low and high index groups were defined using continuous and percentile thresholds of the satellite data—the derived average Normalized Difference Vegetation Index within 250 m and 1,250 m of residence, respectively. During the observation, 3,863 (14%) deaths occurred. The effect of air pollution exposure on mortality was worse in the low index group than in the high index group. Particularly, exposure to SO2 was associated with increased mortality risk, regardless of the greenness threshold. Consistent results were observed in co-pollutant models. High greenery exposure significantly reduced the risk of air pollution related mortality. Our results emphasize the need for creating environmental infrastructures that include green spaces.
Preliminary Study on Human Adipose Stem Cells Promoting Skin Wound Healing Through Notch1 Signaling Pathway
Authors: Wang Yi , Wu Qinchao , Zheng Yang , Wang Chao , Ding Xu , Wu Heming , Wu Yunong , Zhang Wei , Song Xiaomeng 
Published: January 2022

Abstract
Background: Mesenchymal stem cells (MSCs) have been documented as possible candidates for wound healing treatment because their use could reinforce the regenerative capacity of many tissues. Human adipose stem cells (hADSCs) have the advantages of easy access, large quantity and easy operation. They can be fully applied in the treatment of skin wounds. In this study, we aim to explore the roles and potential mechanisms of hADSCs in cutaneous wound healing.Methods: hADSCs were obtained from human subcutaneous fat. Adipocytes and osteocytes differentiated from hADSCs were determined by staining with Oil Red O and alkaline phosphatase (ALP), respectively. We assessed the effects of hADSCs and hADSC conditional medium (CM) on wound healing in an injury model of mice. Than, we investigated the biological effects of hADSCs on human keratinocytes HaCAT cells in vitro.Results: The results showed that hADSCs could be successfully differentiated into osteogenic and lipogenic cells. hADSCs and hADSCs-CM significantly promote skin wound healing in vivo. hADSCs significantly promoted HaCAT cells proliferation and migration through activating Notch1 signaling pathway, and activated the AKT signaling pathway by Rps6kb1 kinase in HaCAT cells. In addition, we found that hADSCs-mediated activation of Rps6kb1/AKT signaling was dependent on the Notch1 signaling pathway.Conclusion: We demonstrated that hADSCs can promote skin cell-HaCAT cells proliferation and migration via Notch1 pathway, suggesting that hADSCs may provide an alternative therapeutic approach for the treatment of skin injury.
Grasp-Squeeze Adaptation to Changes in Object Compliance Leads to Dynamic Beta-Band Communication Between Primary Somatosensory and Motor Cortices
Authors: CU HUY , LYNCH LAURIE , HUANG KEVIN , TRUCCOLO WILSON , NURMIKKO ARTO 
Published: January 2022

Abstract
In asking the question of how the brain adapts to changes in the softness of manipulated objects, we studied dynamic communication between the primary sensory and motor cortical areas when nonhuman primates grasp and squeeze an elastically deformable manipulandum to attain an instructed force level. We focused on local field potentials recorded from S1 and M1 via intracortical microelectrode arrays. We computed nonparametric spectral Granger Causality to assess directed cortico-cortical interactions between these two areas. We demonstrate that the time-causal relationship between M1 and S1 is bidirectional in the beta-band (15-30Hz) and that this interareal communication develops dynamically as the subjects adjust the force of hand squeeze to reach the target level. In particular, the directed interaction is strongest when subjects are focused on maintaining the instructed force of hand squeeze in a steady state for several seconds. When the manipulandum’s compliance is abruptly changed, beta-band interareal communication is interrupted for a short period (~ 1 second) and then is re-established once the subject has reached a new steady state. These results suggest that transient beta oscillations can provide a communication subspace for dynamic cortico-cortical S1-M1 interactions during maintenance of steady sensorimotor states.
WITHDRAWN: The Value of Lung Ultrasound Score Predicts the Ventilator use in Preterm with Dyspnea within 24h after Hospitalization
Authors: zhang lihua , Feng jinnan , jin di , yu zekun , qu mingyang , zheng meiyu , Wu Hui 
Published: January 2022

Abstract
This study aims to explore the predictive value of LUSsc(Lung Ultrasound Score) in the selection of respiratory support mode for premature infants with dyspnea.We prospectively included 857 preterm infants and performed LU in the first 2 hours of admission and scored LUSsc by two specialist sonographers. They were divided into two stratification according to gestational age (<32 +0 weeks and 32 +0 -36+6 weeks), and had two main outcomes: invasive and non-invasive respiratory support. In the training set, analysis the clinical factors finding the best cut-off value of lung ultrasound score then verified the consistency in the verification set. The choice of invasive respiratory support is based on neonatal mechanical ventilation rules. Preterm infants with invasive respiratory support had higher LUS scores and lower OI 、birth weight、than those with non-invasive support. For preterm <32 +0 weeks the cut-off point of LUSsc was 6.5 that the area under curve was 0.749 (95% CI: 0.689-0.809), which was statistically significant (P<0.05), and the sensitivity and specificity were 74.0% and 68.3%, for preterm 32 +0 -36 +6 weeks, cut-off point was 6.5 and the area under curve was 0.863 (95% CI: 0.811-0.911), sensitivity and specificity were 75.3% and 0.836%.In the validation set, use actual clinical respiratory support selection results to verify, for preterm <32 +0 weeks (Kappa value 0.660, P<0.05, McNemar test P ＞0.05),for preterm 32 +0 -36 +6 weeks (Kappa value 0.779, P<0.05, McNemar test P ＞0.05).
Conclusion:
The LUS score shows good reliability to predict respiratory support mode for preterm infants with dyspnea
The Rare Cry For Help: First Record of An Agonistic Call From A Snake In South America
Authors: Fernandes Igor Yuri , Koch Esteban Diego , Mônico Alexander Tamanini 
Published: January 2022

Abstract
Discussions about auditory systems and sound dynamics in snakes are frequent. The known frequency of sounds produced by snakes ranges from 0.2 - 7.5 KHz, ranging from imperceptible sounds to humans to audible and observable squeaks. The hiss and whistles are the most common sound and are not considered vocalizations. During a nocturnal survey on June 13, 2021, in the northern Brazilian Amazon, we observed the first record of vocalization in a South American snake. Emitted by the individual from Dipsas catesbyi has a duration of 0.06 seconds, reaching 3036 Hz in its peak frequency, with an amplitude of 2761 to 4152 Hz of frequency in its main emission. Vocalizations were made during the exhalation of air through the larynx. The modulation differs from all patterns observed for snakes resembling the agonistic call of anuran amphibians, which could characterize an evolutionary mimicry of this behavior. Vocal emission via the larynx can generate internal vibrations perceptible to the auditory system of snakes, which, when vocalizing, vibrate the laryngeal cartilage and vocal cord. Our hypothesis is that structured vocal emission through laryngeal air exhalation may be a characteristic shared by other species of the Colubridae family.
Triamcinolone Acetonide-Loaded Nanoparticles Encapsulated By CD90+ MCSs-Derived Microvesicles Drive Anti-Inflammatory Properties and Promote Cartilage Regeneration After Osteoarthritis
Authors: Li Yuanlong , Tu Qingqiang , Xie Dongmei , Chen Shurui , Gao Kai , Xu Xiaochun , Zhang Ziji , Mei Xifan 
Published: January 2022

Abstract
Background: Osteoarthritis (OA) is a highly prevalent human degenerative joint disorder that has long plagued patients. Glucocorticoid injection into the intra-articular (IA) cavity provides potential short-term analgesia and anti-inflammation, but long-term IA causes loss of cartilage content. Synovial mesenchymal stem cells (MSCs) reportedly promote cartilage proliferation and increase cartilage content. Methods: The CD90+ MCSs-derived micro-vesicle (CD90@MV)-coated nanoparticle (CD90@NP) was developed. CD90+ MCSs were extracted from human synovial tissue. Cytochalasin B (CB) relaxed the interaction between the cytoskeleton and the cell membranes of CD90+ MCSs, stimulating CD90@MV secretion. The poly (lactic-co-glycolic acid) (PLGA) nanoparticle was coated with CD90@MV, and a model glucocorticoid, triamcinolone acetonide (TA), was encapsulated in CD90@NP (T-CD90@NP). Results: CD90@MV membrane proteins were similar to CD90+ MCSs, indicating that the CD90@MV bio-activity is similar to the cartilage proliferation-inducing CD90+ MCSs. The CD90@NP binding to injury cartilage primary cells was significantly stronger than the erythrocyte membrane-coated nanoparticles (RNP). In the rabbit OA model, long-term IA of T-CD90@NP showed significantly enhanced repair of damaged cartilage than TA and CD90+ MCSs treatments. In the rat OA model, short-term IA of T-CD90@NP showed effective anti-inflammatory ability similar to TA treatment. Moreover, long-term IA of T-CD90@NP induced cartilage to restart the cell cycle and reduced cartilage apoptosis. T-CD90@NP promotes regeneration of chondrocytes, reduces apoptosis via the FOXO pathway, and influences type 2 macrophage polarization to regulate inflammation through IL-10. Conclusion: This study confirms that T-CD90@NP promotes chondrocyte proliferation and anti-inflammation, improving the clinical glucocorticoid treatment plan.
Defect Detection of Mechanical Design Products Using Deep Neural Network
Authors: Jan Mian Ahmad 
Published: January 2022

Abstract
In industrial production, defect detection is one of the key methods to control the quality of mechanical design products. Although defect detection algorithms based on traditional machine learning can greatly improve detection efficiency, manual feature extraction is required and the design process is complicated. With the rapid development of CNN, major breakthroughs have been made in computer vision. Therefore, building a surface defect detection algorithm for mechanical design products based on DCNNs plays a very important role in improving industrial production efficiency. This paper studies the surface defect detection algorithm of mechanical products based on deep convolutional neural network, focusing on solving two types of problems: defect recognition and defect segmentation. Aiming at the problem of defect recognition, this paper studies a defect recognition algorithm based on fully convolutional block detection. This algorithm introduces the idea of block detection into the ResNet fully convolutional neural network. While realizing the local discrimination mechanism, it overcomes the shortcomings of the traditional block detection receptive field. Compared with the original ResNet image classification algorithm, this algorithm has stronger generalization ability and detection ability of small defects. Aiming at the problem of defect segmentation, this paper studies a defect segmentation algorithm based on improved Deeplabv3+.
Increased Levels of PD1 and Glycolysis in CD4+ T Cells Promote Lymph Node Metastasis in Oral Squamous Cell Carcinoma Patients
Authors: Wu Kun , Han Nan-nan , Zhang Sheng , Li Yan 
Published: January 2022

Abstract
Background: Cervical lymph node metastasis is one of the poorest prognostic factors in oral squamous cell carcinoma (OSCC). Activated immune cells and cancer cells generally have metabolic similarities in tumor microenvironment. However, it is unknown whether abnormal glycolysis in T cells could facilitate metastatic lymph nodes in patients with OSCC. Methods: Flow cytometry and immunofluorescence staining were used to analyze the differences in CD4+ PD1+ T cells between metastatic and negative lymph nodes. RT-PCR was performed to detail the expression of immune checkpoints and glycolysis-related enzymes in metastatic and negative lymph nodes. Kruskal-Wallis, Mann-Whitney, or nonparametric paired tests (i.e., the Wilcoxon matched paired test) were used to analyze the non-parametric distribution of the samples. Results: The frequency of CD4+ T cells decreased in the metastatic lymph nodes (p = 0.0019). Immune checkpoints (PD1, PDL1, and CTLA4) of CD4+ T cells were detected in metastatic (LN+) and paired negative lymph nodes (LN-) of OSCC patients. The PD1 expression of LN+ increased markedly compared to that of LN- (p = 0.0205). Similarly, the PD1 of CD4+ T cells in LN+ increased significantly compared to that of LN-. Glycolysis-related enzyme levels in CD4+ T cells from LN+ were dramatically higher than those in LN-. Moreover, PD1 and Hk2 expressions in CD4+ T cells increased in metastatic lymph nodes of OSCC patients with prior surgical treatment compared to those without. Conclusions: These findings suggest that increased PD1 and glycolysis in CD4+ T cells may serve as pivotal regulators of OSCC metastatic lymph nodes, which are closely associated with elevated glycolysis.