bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding Perinatal Women’s Health Preferences and Patient-Provider Relationships to Prevent Congenital Syphilis in California and Louisiana
Authors: Wagman Jennifer Ann , Park Eunhee , Giarratano Gloria P. , Buekens Pierre M. , Harville Emily W 
Published: January 2022

Abstract
Background

Congenital syphilis (CS) has reemerged as a global maternal and child health crisis. Kern County, California and East Baton Rouge Parish, Louisiana are among the highest CS morbidity regions in the United States. We previously reported on social-ecological and structural barriers to prenatal care and maternal syphilis testing and treatment in these two regions. The aim of this study was to examine perinatal women’s health preferences and perceptions of patient-provider relationships in the prenatal care clinic setting.
Methods

Between May 2018 and January 2019 we conducted 20 in-depth qualitative interviews with prenatal providers and 8 focus group discussions with pregnant and postpartum women in Kern County and East Baton Rouge Parish. We applied an adapted health services framework to analyze participants’ understanding of health disparities and vulnerable populations; perinatal women’s health and prenatal care preferences; and participants’ perspectives of clinical encounters in the context of prenatal care and maternal syphilis testing and treatment.
Results

Site-specific determinants of syphilis infection emerged but participants from both locations felt CS prevention efforts should be prioritized among youth, racial/ethnic minority populations, people experiencing socioeconomic limitations and people with other commonly occurring health conditions. Although perinatal patients expressed clear health preferences, they reported inconsistent receipt of respectful, patient-centered care. Inconsistencies were connected with limited ethnic and cultural competence among providers, and implicit, negative attitudes toward patients using substances, experiencing homelessness, or engaging in sex work. Providers clearly aimed to offer high quality prenatal care. However, some clinic and health systems level factors were thought to reduce positive and communicative patient-provider relationships, contributing to gaps in use of prenatal care and syphilis testing and treatment.
Conclusions

Our findings suggest that interventions tailored to address setting-specific determinants (including clinic and health system factors) of disparities in CS risk could improve pregnant women’s access to prenatal care and ensure they and their sex partners receive timely syphilis screening and treatment. We recommend all prenatal care providers receive training on how to identify and mitigate implicit biases and provide competent and compassionate patient-centered care.
Determination of content analysis of Achillea arabica Kotschy. plant flower ethanolic extract by LC-MS/MS and NMR and revealing the curative effects of the extract in diabetic rats
Authors: Hanalp Hanife Ceren , Dogan Abdulahad , Saygi Tuba Kusman 
Published: January 2022

Abstract
Background Achillea arabica is a perennial aromatic herb widely used in folk medicine for the treatment of the stomachache and abdominal pain, wound healing, gout, cancer, diabetes and such. The aim of this study was to determine the photochemical content of the ethanolic lyophilized extract of A. arabica flower by LC-MS/MS and NMR and to reveal the antidiabetic and antioxidant effects of this extract against streptozotosine (STZ)-induced in diabetic rats.Methods and Results After toxicity test, 35 female rats were divided into 5 groups. Control, Diabetes mellitus (DM), A.arabica (400 mg/kg) extract, DM +A.arabica (400 mg/kg) extract and DM+Glibenclamide (2 mg/kg). According to LC–MS/MS results of A. arabica flower extract, quinic acid (2439.9 μg/g), cyranoside (858.4 μg/g), chlorogenic acid (698.7 μg/g) and cosmosin (347.8 μg/g) were determined as major compounds, respectively. In addition, two new compounds were determined in this plant according to NMR analyzes and these compounds were named edremitine and achillosine, respectively. It was determined that while diabetic rats fed A. arabica plant extract significantly decreased blood glucose level, serum glucose, HbA1c, liver and kidney damage biomarker levels, and malondialdehyde (MDA) content compared to the DM group, it caused fluctuations in antioxidant enzyme levels.Conclusion We conclude that A. arabica flower plant extract may be a good addition to the well established pharmacological approach of DM by contributing to the reduction of overall oxidative load.
Biochemical Characteristics of Point Mutated Goat Lysosome Α- Mannosidase
Authors: wang yan , Zhang Jiangye , Xiong Haofei , Li Qinfan 
Published: January 2022

Abstract
Background Locoweeds are widely distributed all over the world. Goats and other herbivores often get poisoning if they eat a large number of locoweeds by mistake. Swainsonine (SW), the main toxin in locoweeds, can inhibit lysosomes α-Mannosidase (LAM) competitively in animal cells. The insufficient activity of lysosomes α-Mannosidase can lead to abnormal metabolism of animals, forming alpha mannosidosis. However，the details of interaction between SW and LAM are not clear yet. Methods In this study, molecular docking was used to predict the interaction points between SW and LAM. The effect of putative points was investigated by constructing mutated LAM (LAMM) and analyzing its biochemical characteristics. Results The results showed that the Trp at the 28th position and Tyr at the 599th position of the LAM were the candidates of interaction points. Both tryptophan at position 28 of A peptide residue and tyrosine at position 599 of D peptide residue of goat LAM were mutated into glycine, and goat lysosomes were obtained α- The mutant sequence of mannosidase; The recombinant yeast GS115 / pPIC9K LAMM was successfully constructed; After SW induction, the sensitivity of goat LAMM to SW decreased significantly, and the enzyme activity decreased about 3 times compared with wild-type LAM; The optimum temperature of LAMM decreased from 55 °C to 50 °C, and the optimum pH value increased from 4.5 to 5.0; All the trivalent metal ions (Fe3+, Al3+, Cr3+) had significant activation (P < 0.05), and EDTA had inhibitory effect on goat LAM before and after mutation. Conclusions These results indicated that the sensitivity of goat LAMM to SW was significantly reduced, but other characteristics changed little, which was consistent with the molecular docking results of goat LAMM. This study provides help for the study of interaction mechanism between SW and goat LAM, and also provides a new idea for the development of animal locoweed disease control technology.
Relationship Between Sedentary Behavior And Sleep Indicators In Patients With Chronic Schizophrenia
Authors: Takenouchi Rei , Shibata Masashi , Kishida Ikuko , Ishii Chie , Ishii Norio , Kawada Miwa , Buyo Momoko 
Published: January 2022

Abstract
Background: To investigate the relationship between daytime sedentary behavior and physical activity and sleep in patients with chronic schizophrenia, particularly focusing on insomnia symptoms, a hallmark of insomnia in patients with schizophrenia.Method: The subjects were 20 patients with chronic schizophrenia more than 1 year after onset (12 men and 8 women with a mean age of 59.0 ± 9.8 years), of whom 4 were outpatients and 16 were inpatients. Sedentary behavior performance and physical activity index (Total sedentary behavior time and sedentary behavior bout number, low-intensity physical activity, MVPA, and steps) were assessed by wearing a triaxial accelerometer (GT3X-BT). Total bed time (TIB), total sleep time (TST), sleep latency (SIL), wake after sleep (WASO), and sleep efficiency (SE) were calculated using a small body dynamometer as an objective sleep index. Both the triaxial accelerometer and the small body dynamometer were worn for 1 week, and the average value of each index was calculated.Results: Total sedentary behavior time was significantly positively associated with TIB and SL (r =. 499, p <. 05; r =. 543, p <. 05). Sedentary behavior bout number was negatively significantly associated with SE (r = -. 592, p <. 01) and positively associated with WASO (r =. 503, p <. 01). MVPA was significantly positively associated with SE (r =. 536, p <. 05) and negatively associated with WASO (r = -. 58, p <. 01).Conclusion: Daytime sedentary behavior in patients with chronic schizophrenia was associated with insomnia symptoms. It was suggested that decreasing their sedentary behavior time and increasing moderate-to-vigorous intensity activities may improve their sleep quality.In this study, though the relation has not been recognized in CP conversion value and sedentary behavior position action index, the relation between antipsychotic dose and total sedentary behavioral time needs further investigation.
WITHDRAWN: A Digital Dial Recognition Method Based on Lightweight ResNet
Authors: Zheng Gongming , Wang Zhiwei 
Published: January 2022

Abstract
This paper proposes a lightweight residual network for highly accurate recognition of the numbers of wheel dials. The proposed residual network is based on an optimized ResNet-18 network with the depthwise separable convolution and Dropout function. In addition, a case study on automatic water meter readings based on captured images is presented. Compared to existing methods, the results demonstrate improved recognition accuracy as high as 99.8% and increased computation speed with reduced model parameter size.
Development of An Intraoperative Breast Cancer Margin Assessment Method Using Quantitative Fluorescence Measurements
Authors: Ueo Hiroki , Minoura Itsushi , Ueo Hiroaki , Gamachi Ayako , Kai Yuichiro , Kubota Yoko , Doi Takako , Yamaguchi Miki , Yamashita Toshinari , Tsuda Hitoshi , Moriya Takuya , Yamaguchi Rin , Kozuka Yuji , Sasaki Takeshi , Masuda Takaaki , Urano Yasuteru , Mori Masaki , Mimori Koshi 
Published: January 2022

Abstract
Breast-conserving surgery has become the preferred treatment method for breast cancer. Surgical margin assessment is performed during surgery, as it can reduce local recurrence in the preserved breast. Development of reliable and lower-cost ex vivo cancer detection methods would offer several benefits for patient care. Here, a practical and quantitative evaluation method for the ex vivo fluorescent diagnosis of breast lesions was developed and confirmed through a three-step clinical study. Gamma-glutamyl-hydroxymethyl rhodamine green (gGlu-HMRG) has been reported to generate fluorescence in breast lesions. Using this probe, we constructed a reliable and reproducible procedure for the quantitative evaluation of fluorescence levels. We evaluated the reliability of the method by considering reproducibility, temperature sensitivity, and the effects of other clinicopathological factors. The results suggest that the fluorescence increase of gGlu-HMRG is a good indicator of the malignancy of breast lesions. However, the distributions overlapped. A 5 min reaction with this probe could be used to distinguish at least part of the normal breast tissue. This method did not affect the final pathological examination. In summary, our results indicate that the methods developed in this study may serve as a feasible intraoperative negative-margin assessment tool during breast-conserving surgery.
Individual and Community-level determinants of maternal health services utilization in Northwest Ethiopia: Multilevel Analysis
Authors: Amentie Muluwas 
Published: January 2022

Abstract
Objective

Utilization of maternal health services is a direct and indirect indicator of perinatal death, and socio-economic development. Evidence on individual and community level determinants of maternal health services in Ethiopia, particularly in the study region was not found. Hence, this study fills this gap.
Results

Among 2,198 study subjects, proportion of pregnant women who visited antenatal care (ANC4+), received skilled delivery care, and postnatal care (PNC) were 66.1%, 58.3%, and 58.6% respectively. Besides these, community and different individual-level factors for the three indicators were detected. For ANC 4th visits: place of resident (AOR=3.82), information on MHS (AOR=2.13), history of pregnancy-related problems (AOR=1.83) and women's decision-making (AOR=1.74). For skilled delivery were belonged in 3rd quintile household wealth (AOR=2.23), women's education (AOR=1.71), attended ANC visit 4th (AOR=2.29), delay initiation of ANC visit between 4–6 months (AOR=0.66). Finally, for PNC services: partner education (AOR=3.67), attending recommended ANC visits (AOR=10.8), iron folic acid supplementation (AOR=1.96) and skilled delivery (AOR=1.63). Hence, community based interventions are strongly recommended to improve utilization of maternal health services.
Comparison of on-treatment Noninvasive Markers of Liver Fibrosis Among Patients with HBV and HBV/HIV Coinfection in Ethiopia
Authors: Sultan Amir , Amogne Wondwossen 
Published: January 2022

Abstract
Background: Noninvasive markers of liver fibrosis have gained central importance in the assessment of patients with liver diseases. Among patients with Hepatitis B and HIV coinfection, the role of these noninvasive serum markers has not been well studied in the Ethiopian setup. Methodology: A hospital-based cross-sectional study was conducted at Tikur Anbessa Hospital ART clinic. Patients with HIV and HBV coinfection were recruited from the clinic, and a second comparison group with HBV mono-infection was recruited from the liver clinic. All patients were on tenofovir-based treatment of hepatitis B. Baseline demographic and laboratory values comparison between the two groups were conducted. For APRI, a cutoff of 0.5 and 1.5 was used to assess fibrosis; and for FIB4, cutoffs of 1.45 and 3.25 were used as the lower and higher cutoff, respectively. Result: A total of 74 patients were included, of which 24 patients were with HBV/HIV coinfection and 50 were with HBV mono-infection. The median age of participants was 37, and the majority were from Addis Ababa. Prevalence of diabetes and alcohol use was comparable in both groups. The median platelet count among patients with coinfection was 220,000 ( IQR 159000 - 277000), while among the monoinfected patients, it was 166000 ( 87750-227500). The APRI score among the coinfected patients was 0.4 ( 0.23 – 0.59) while it was 0.57 (0.4-1.4) ( p= 0.01). The FIB4 was 1.1 ( 0.69 – 2.03 ) among coinfected patients, while it was 1.7 (1 - 2.9) among monoinfected patients ( p=0.01). Using a lower and higher APRI and FIB4 cutoffs, both groups had a statistically nonsignificant difference. However, the platelet count using a cutoff of 1500000 was significantly lower among patients with HBV mono-infection. Conclusion; Our data indicate that patients with HIV/HBV coinfection on treatment had comparable noninvasive liver fibrosis marker profiles compared to patients with HBV mono-infection on therapy. On the majority of the parameters, there was a trend towards a better profile among patients with coinfection, which might be explained by early initiation of tenofovir therapy as part of HIV treatment.
Regional Homogeneity And Homotopic Connectivity Changes In Breast Cancer Survivors With Fear of Cancer Recurrence: A Resting State FMRI Study
Authors: Peng Li , Hu Xiaofei , Xu Chen , Yang Ying , Liu Ju , Chen Jiannan , Qi Aiping , Xue Yuan , Zhong Lin , Li Min 
Published: January 2022

Abstract
Background: Fear of cancer recurrence(FCR) has been one of the most commonly endorsed unmet need for breast cancer survivors, but the neuroimaging mechanism underlying fear of cancer recurrence still remains unclear. Methods: we recruited 62 breast cancer survivors (31 in high FCR group and 28 in low FCR group) to explore the inter-group differences in neuroimaging findings. All the participants underwent resting-state functional magnetic resonance imaging (rs-fMRI) scan. Regional homogeneity (ReHo) and Voxel-mirrored homotopic connectivity (VMHC) method were applied to analyze the imaging data. Results: Breast cancer survivors with high and low FCR showed significantly different ReHo in the left caudate nucleus and precuneus, and significantly different VMHC in brain regions in the posterior cerebellar lobe, the superior frontal gyrus, the orbital part, the inferior frontal gyrus, the occipital gyrus, the inferior parietal lobule and the frontal middle gyrus. The left caudate nucleus was negatively correlated with FCR (p < 0.05), and the correlation coefficient was -0.501. The right precuneus lobe was positively correlated with FCR scores (p < 0.05), and the correlation coefficient was 0.505. Besides, VMHC in bilateral superior occipital gyrus was positively correlated with the total score of FCR (p < 0.05), and the correlation coefficient was 0.438Conclusion: This study suggested that the left caudate nucleus, right precuneus lobe and bilateral superior occipital gyrus were involved in the neuropathology of FCR. ReHo and VMHC in specific regions could be applied as a biomarker to distinguish breast cancer survivors with high FCR from those with low FCR.
Estimating F-statistics Using Non-independent Samples
Authors: Huang Kang , Yang Bing , Li Yuhang , Ao Jincuo , Dunn Derek W. , Li Baoguo 
Published: January 2022

Abstract
Existing F-statistic estimators fail to account for any genetic correlations among individuals or subpopulations and assume that all samples are independent. This may result in inaccurate F-statistic estimations for natural populations. Here, we derive the expectations of previous F-statistics estimates using extended kinship coefficients. On this basis, we developed a new method for F-statistic estimation that accounts for non-independence of samples, finite sample sizes, and autopolyploidy. As proof of principle, using the same simulated datasets we compared the accuracy of several established F-statistic estimators with our new estimator. We found that our new method outperformed all of the other methods we used and showed almost no bias. Our new method has been added as a new function to our existing software package polygene V1.4, which is freely available at http://github.com/huangkang1987/polygene.
An Early Warning Risk and Control Model for Manpower Capital Investment Using Data Warehousing and Decision Support System
Authors: Chakrabarty Vinky 
Published: January 2022

Abstract
Human capital plays an important role in the development of enterprises. Investing in human capital is the main focus of enterprises to improve personnel quality and enhance their core competitiveness. With the development of market economy, the function of human resources market allocation has been improved, and the mobility of enterprise human resources has been enhanced leading to the increase of investment risk of enterprise human capital. Enterprise human capital investment risk has negative impact for enterprises, reduces the income of enterprise’s human capital investment and affect their growth. Hence, enterprises need to avoid the risk of human capital investment, or minimize the negative impact of risk. Using the data warehouse and decision support system, this paper constructs the early warning and control model for human capital investment risk, analyzes the existing approaches during the recruitment process and training, investment and production, and among enterprises. Finally, this paper proposes the corresponding control method according to the model inspiration.
Prenatal Diagnosis of Isovaleric Acidemia from Amniotic Fluid Using Genetic and Biochemical Approaches
Authors: Ding Si , Liang Lili , Qiu Wenjuan , Zhang Huiwen , Xiao Bing , Dong Liping , Ji Wenjun , Xu Feng , Gong Zhuwen , Gu Xuefan , Wang Lei , Han Lianshu 
Published: January 2022

Abstract
Background：Isovaleric acidemia (IVA) is an inborn error of leucine metabolism and different approaches have been applied to its prenatal diagnosis. However, systemic application of a biochemical strategy is rare. To evaluate its reliability and validity, we conducted a retrospective study of our experience with metabolite measurement together with genetic analysis in IVA prenatal diagnosis at a single center.Methods：A total of eight pregnancies whose probands were diagnosed as IVA were referred to our center for prenatal diagnosis. Prenatal data of genetic analysis and metabolite measurement using tandem mass spectrometry (MS/MS) and gas chromatography/mass spectrometry (GC/MS) in amniotic fluid (AF) samples were retrospectively reviewed. Results: Genetic and biochemical results were both available in these eight at-risk fetuses. Among them, two fetuses had higher levels of isovalerylcarnitine (C5) and C5/acetylcarnitine (C2) in AF compared with normal reference range and, thus, were determined to be affected, both of whom were found to carry compound heterogeneous mutations according to genetic analysis. The remaining six fetuses were determined to be unaffected based on a normal AF metabolite profile, except one showed slightly elevated C5 and they were found to be carriers according to genetic analysis. However, the level of isovalerylglycine (IVG) could not be detected at all in both groups. The diagnostic sensitivities of C5 and C5/C2 were both 100%, and the specificities were 83.3% and 100%, respectively. Conclusions: The biochemical analysis, as a quick and convenient method, could be an additional reliable option for the prenatal diagnosis of IVA, especially in families with inconclusive genetic results, and can achieve a more precise diagnosis in conjunction with mutation analysis. Among the three tested biochemical markers, C5/C2 appears to be the most credible.
N6-methyladenosine-mediated SH3BP5-AS1 upregulation promotes GEM chemoresistance in pancreatic cancer by competitively sponging miR-139-5p and activating the Wnt signaling pathway
Authors: Lin Chengjie , Wang Yan , Dong Yihong , Lai Shihui , Wang Liang , Zhang Xiang , Weng Shangeng 
Published: January 2022

Abstract
Background

Pancreatic cancer (PC) is highly malignant. Chemotherapy is the main treatment strategy, especially for patients with advanced PC. However, chemoresistance has always been a frequently encountered bottleneck. Hence, there is an urgent need to enhance the sensitivity of PC to gemcitabine (GEM).
Methods

A patient-derived xenograft model was used for screening the hub lncRNAs affecting GEM chemoresistance. Transwell and colony formation assays were used to assess the migration, invasion, and stemness of PC cells. qRT-PCR and western blot analyses were performed to detect the mRNA and protein levels indicated in this study, respectively. The MTT assay was performed to assess the viability of PC cells in vitro. MeRIP-qPCR was used to determine the N6-methyladenosine (m6A) modification level. RIP and pull-down were used to investigate the interaction between SH3BP5-AS1 and miR-139-5p, as well as the interactions among SH3BP5-AS1, ALKBH5, and IGF2BP1. The functional mechanism of SH3BP5-AS1 in PC was identified by gain- and loss-of-function assays and high-throughput sequencing in vitro.
Results

We demonstrated that SH3BP5-AS1 was significantly upregulated in GEM-resistant PC and predicted a poorer prognosis. SH3BP5-AS1 stability was regulated by ALKBH5/IGF2BP1-mediated m6A modification. Loss of SH3BP5-AS1 reduced PC cell migration and invasion and enhanced the sensitivity of PC to GEM, as confirmed by gain- and loss-of-function assays in vitro and in vivo. Bioinformatics analysis revealed that SH3BP5-AS1 acted as a ceRNA against miR-139-5p and directly targeted CTBP1, affecting the biological behavior of PC cells. The mechanistic studies revealed that the upregulation of SH3BP5-AS1 increased CTBP1 expression by directly activating the Wnt signaling pathway, promoting GEM resistance.
Conclusion

This study revealed that SH3BP5-AS1 activated Wnt signaling pathway by sponging miR-139-5p, upregulating CTBP1 expression, and contributing to the sensitivity of PC cells to GEM. SH3BP5-AS1 might be a potential target for PC therapy.