bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards a productivity-based management of tropical reefs in the Anthropocene
Authors: SEGUIN Raphael , Mouillot David , Cinner Joshua , Stuart-Smith Rick , Maire Eva , Graham Nicholas , McLean Matthew , Vigliola Laurent , Loiseau Nicolas 
Published: March 2022

Abstract
Tropical reefs provide livelihood and food security to millions of people. However, tropical reefs are entering new configurations requiring management strategies to incorporate process-oriented measures such as biomass production to determine their dynamics and resilience. We proposed a new framework, comprising three management strategies based on fish standing biomass and productivity which were calculated on 2,027 reef sites over 39 countries. We found that 3.5% of the sites have low fish biomass and productivity, where alternative fisheries like seaweed and bivalve aquaculture are recommended. We identified 24% of high productivity sites where we suggest adaptive management: establishment of partially protected areas sustaining local fisheries when needed, or full protection when possible given their capacity for recovery. For the 5% high biomass sites, we urgently need full protection to maintain high reproductive capacity through large individuals. Our framework can thus guide management strategies by incorporating both static and process-oriented measures, tailoring each strategy to local priorities based on biodiversity and socio-economic objectives.
Study of Freeze-thaw Induced Damage Characteristic for Himalayan Schist
Authors: Verma Amit , Sardana Sahil , Jaiswal Amit 
Published: March 2022

Abstract
The freeze-thaw (F-T) induced deterioration in rocks affects their durability, leading to various landslides, rockslides, and avalanches. The study focuses on estimating loss in rock strength using mineralogical properties under the influence of multiple F-T cycles. The rock samples were collected from F-T affected north Indian Himalayan region. The rock specimens were subjected to multiple F-T conditioning (0th, 10th, 20th and 30th F-T cycles). The mineralogical properties (such as mineralogical indices and textural indices) and mechanical properties (such as uniaxial compressive strength; UCS and Brazilian tensile strength; BTS) were calculated before and after F-T conditioning. A correlation was developed among the various strength properties and mineralogical properties. The result shows a strong relationship between the UCS and BTS with mean grain size. Further, results were used to develop a mathematical model, based on mineralogical parameter and decay function, to predict the rock strength under the influence of multiple F-T cycles.
A rare case report: ICOS and WIPF1 mutation together in a patient
Authors: Arik Elif , Keskin Ozlem , Cesur Mahmut , Kucukosmanoglu Ercan , Basturk Ahmet 
Published: March 2022

Abstract
Background and Aims: We report a girl with novel inducible co-stimulator (ICOS) and novel WIPF1 mutations. Methods: A 12 year old female patient presented with bloody diarrhea, weight loss, swelling in both knees that started two weeks ago. In the history of the patient: she had convulsions, stayed in the hospital for 3 months due to peeling and bleeding of her skin at the age of 6 months, started to have otitis media 20 times a year at the age of 2. At the age of 9, she started to have diarrhea and had thrombocytopenia causing intracranial bleeding. In her family history, her parents were cousins, her aunt died due to bowel disease, two of her uncles died due to leukemia, both grandfathers had thrombocytopenia. In the physical examination; Her height and weight were <3persentil, diffuse crepitant ral was present in her lungs, she had splenomegaly, arthritis and clubbing. Results: Ig G (190 mg/dl) and Ig A (<60 mg/l) levels were low, Ig M, CD3, CD4, CD8, CD19, CD16/56 were within the age-appropriate range, switched and unswitched memory B cells were decreased. The immune dysregulatory manifestations were controlled by corticosteroids, salazopyrin and intravenous immunoglobulin. Immunodeficiency genetic panel with next generation clinical sequencing: WIPF1(c.310T>C) and ICOS (c.221_222delCA) homozygous mutations. Conclusions: ICOS deficiency was first described in 2003; characterized with recurrent sinopulmonary infections, autoimmunity, inflammatory bowel disease and malignancy. WIPF1 mutation causes Wiscot-Aldrich syndrome type2, characterized by thrombocytopenia, immunodeficiency and eczema. Two rare genetıc diseases in the same patient should not be a surprise for regions with high consanguineous marriages.
Extraction, purification, characterization and evaluation of antibacterial and anticancer activity of β,ε-Carotene-3,3′-diol (Lutein) from Bacillus endophyticus- AVP9
Authors: Mantri Sairam , Syed Chandini Sultana , Konatham Samuel John , P Ponraj , Yalpi Karthik , Audipudi Amrutha Valli , Ramakrishna Krishnaveni 
Published: March 2022

Abstract
A non-fluorescent pigment produced from the fluorescent Bacillus endophyticus AVP-9 which possess abundant lutein content considered as a xanthophyl-type compound with potentially beneficial pharmacological properties for enhancing human health. In this context, bacterial lutein was extracted by employing different solvents to isolate from Bacillus endophyticus was examined in this study. Chromatographically purified lutein was spectrally characterized using ultraviolet-visible spectrometry and spectral analysis was carried through liquid chromatography-mass spectrometry, functional group identification was done by using fourier transform-infrared spectroscopy, structural determination by nuclear magnetic resonance spectrometry. By using hexane and ethyl acetate (7:3) with the flow rate of 5ml/min, was proven to be an excellent solvent phase for separation of lutein, meaning that up to 90% pure lutein generated with a 60% estimated yield. The purified compound identified as a β, ε-3, 31-diol (lutein) having molecular weight of 568.6g/mol with the molecular formula C40H56O2 which exhibits potential antibacterial activity against E. coli (35mm) followed by S. typhi (33mm), S. flexneri (30mm), P. aureginosa (29mm), M. lutea (28mm), P. vulgaris (24mm), S. aureus (21mm) and lowest antibacterial activity against V. cholera (19mm). Purified pigment shows concentration dependent antioxidant activities with IC50 values of pigment was found to be 4.5 mg/ml. Whereas anti-proliferation activity pigment shows considerable effect MCF-7 and LNCaP cell lines, when treated with 100µg/ml, cell viability was decreased to 36.363% at IC50 value of 21.935 µg/ml and for LNCaP, the cell viability was decreased to 42.449% at IC50 value of 56.937 µg/ml.
Conservation of Malus Niedzwetzkyana Dieck Ex Koehne Genotypes From Kazakhstan Resistant to Scab and Fire Blight Diseases
Authors: Nurtaza Aidana , Pozharskiy Alexandr , Dyussembekova Damira , Dolgikh Svetlana , Nizamdinova Gulnaz , Taskuzhina Aisha , Kakimzhanova Almagul , Gritsenko Dilyara 
Published: March 2022

Abstract
Southeast Kazakhstan is part of the center of origin and the natural habitat of wild apple trees. Wild apples and their hybrids are involved in domestic apple breeding as a source of resistance to biotic and abiotic stress. Malus niedzwetzkyana is a valuable endemic apple species included in the Red Book of Endangered Species of Kazakhstan. The present study evaluated 23 M. niedzwetzkyana genotypes from different populations for their resistance to scab and fire blight diseases using SCAR markers. We determined that all 23 genotypes contained a 430 bp resistance allele for marker OPB19, and 5 of them also had a 799 bp resistance allele identified by marker OPB18 for scab resistance. For fire blight, seven genotypes contained a 375 bp resistance allele and one genotype had a 397 bp resistance allele for the QTL FBF7 locus identified by markers AE10-375 and GE-8019, respectively. Eight genotypes with double resistance to scab and fire blight were selected for further in vitro amplification to develop a genetic pool for biodiversity preservation, apple breeding, and wild apple population revival, among other purposes. All in vitro regenerants were analyzed for the presence of apple chlorotic leaf spot virus, apple mosaic virus, apple stem grooving virus, apple stem pitting virus, and tomato ringspot virus. For each of the eight genotypes with double resistance, genetic profiles were developed based on 12 SSR markers. These genetic passports allow us to maintain the purity of the genetic pool and improve MAS in apple breeding.
Management Strategies to De-Implement Low-Value Care – An Applied Behavior Analysis
Authors: Ingvarsson Sara , Hasson Henna , Augustsson Hanna , Nilsen Per , Schwarz Ulrica von Thiele , Sandaker Ingunn 
Published: March 2022

Abstract
Background: There is a lack of knowledge about management strategies being used to de-implement low-value care (LVC). Furthermore, it is not clear from the current literature what mechanisms are involved in such strategies and how they can change physicians’ behaviors. Understanding mechanisms is important for determining a strategy’s potential impact. Applied behavior analysis focuses on processes involved in increasing and decreasing behaviors. Therefore, the aim of this study is to understand what management strategies are being used to de-implement LVC and the possible mechanisms involved in those strategies, using concepts from applied behavior analysis.Method: We applied a qualitative study design using an inductive approach to understand what management strategies are in use, then employed applied behavior analysis concepts to deductively analyze the mechanisms involved in them.Results: We identified eight different management strategies intended to influence LVC. Five of the strategies were developed at a regional level and had the potential to influence physicians’ LVC-related behaviors either by functioning as rules on which LVC to de-implement, or by initiating local strategies in each health care center that in turn could influence LVC practices. The local strategies had a stronger potential for influencing de-implementation. Conclusion: Both strategies at a systemic level (regional) and on a local level (health care centers) must be considered to influence LVC-related behaviors. Strategies at the center level have a specific opportunity to impact LVC-related behaviors because they can be tailored to specific circumstances. Using applied behavior analysis to understand these circumstances can be helpful for tailoring strategies to reduce LVC use.
Organ of Corti vibrations are dominated by longitudinal motion in vivo
Authors: Meenderink Sebastiaan , Dong Wei 
Published: March 2022

Abstract
Recent observations of sound-evoked vibrations in the cochlea’s sensory organ of Corti (ooC), using optical coherence tomography (OCT), revealed unanticipated and complex motions. Relative motion between different structures of the ooC is the key for mammalian ears to achieve remarkable frequency selectivity and sensitivity but is not well understood, especially in the low-frequency region that is essential to human speech perception. We mapped the ooC motions using multiple OCT viewing angles over an extended region in the low-frequency apex of gerbil cochleae. These resolve the “direction limit” with classical approaches and address low-frequency sound processing in the mammalian ear. Our data demonstrate the active structure of sensory outer hair cells moves with the traveling wave. In addition, they also move along an unexpected longitudinal direction. This motion is never predicted, adds another dimension to address the active process within the cochlea, and impacts the interpretation of cochlear vibrometry data.
Assessing microplastic dispersion from Saigon urban canals via Can Gio Mangrove Reserve to East Sea in Vietnam by Raman scattering microscopy
Authors: KHUYEN VO THI KIM , Le Dinh Vu , Anh Le Hung , Fischer Axel René , Dornack Christina 
Published: March 2022

Abstract
Plastic pollution is one of the significant environmental concerns due to the threefold increase in global plastic waste. Marine microplastics, including petroleum-based plastic pieces, synthetic and artificial fibers smaller than 5 mm, are ubiquitous in natural water environments due to the degradation of plastic products and wastes. This research aims to investigate the microplastic presence in urban and coastal environments in the Southeast regions of Vietnam by using Raman microscopy. As a result, most common plastics (PE, PET, PA, PP, PVC, PS and PMMA) were detected, and most of them were fibrous smaller than 500 µm. The total microplastics decreased gradually from the urban waterborne (up to 220 MPs/L) via Can Gio UNESCO Mangrove Biosphere Reserve (10 MPs/L) and to the East Sea (3 MPs/L), which reveals the potential role of the mangrove in reducing marine contaminants including microplastics. This study provides important insights about microplastic pollution in the Western Pacific Region, especially Saigon-Dong Nai river systems, supporting the useful data for natural water resources management.
Association between 25-hydroxyvitamin D and abdominal aortic calcification in US adults: Findings From NHANES 2013-2014
Authors: Wang Min , Wang Bin , Chao Ce , Zhou Rui , Zhan Xianghong , Qian Yongxiang , Di Dongmei 
Published: March 2022

Abstract
Aims: This study aimed to explore the correlation of 25-hydroxyvitamin D and abdominal aortic calcification.Methods: In this cross-sectional study, the data were collected from NHANES (National Health and Nutrition Examination Survey) 2013–2014. Multivariable logistic regression was performed to determine the association of 25-hydroxyvitamin D and AAC. The non-linear relationship was analyzed using smooth curve fitting. Results: 3,040 participants with compete data were included for data analysis. The fully-adjusted model showed serum calcium and HDL levels were correlated with the severity of AAC, and 25-hydroxyvitamin D was closely correlated with plasma calcium and HDL levels. Furthermore, either a low or high level of 25-hydroxyvitamin D was positively correlated with an increased risk of severe AAC, and a U-shaped dose-response relationship (inflection point: 45.8ng/mL and 146ng/mL) was indicated. Conclusions: Either a high or low level of 25-hydroxyvitamin D was positively associated with an increased risk of severe AAC. It was suggested that clinicians should be cautious when prescribing 25-hydroxyvitamin D supplementation, and the mechanism underlying the correlation of 25-hydroxyvitamin D and AAC remains to be further confirmed.
Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin
Authors: Zhang Tao , Yang Han , Zhou Zenan , Bai Yongtai , Wang Jiadong , Wang Weibin 
Published: March 2022

Abstract
DNA end resection is delicately regulated through various types of post-translational modifications to initiate homologous recombination, but the involvement of SUMOylation in this process remains incompletely understood. Here, we show that MRE11 requires SUMOylation to shield it from ubiquitin-mediated degradation when resecting damaged chromatin. Upon DSB induction, PIAS1 promotes MRE11 SUMOylation on chromatin to initiate DNA end resection. Then, MRE11 is deSUMOylated by SENP3 mainly after it has moved away from DSB sites. SENP3 deficiency results in MRE11 degradation failure and accumulation on chromatin, causing genome instability. We further show that cancer-related MRE11 mutants with impaired SUMOylation exhibit compromised DNA repair ability. Thus, we clarify that MRE11 SUMOylation in coordination with ubiquitylation is dynamically controlled by PIAS1 and SENP3 to facilitate DNA end resection and maintain genome stability.
KOH electrochemical modification of fluorinated graphite and its reaction mechanism
Authors: Li Hao , Yuan Xiaojing , Hou Genliang , Bi Song , Song Yongzhi , Wang Xuping 
Published: March 2022

Abstract
The KOH electrochemical method and heating method were employed to modify ﬂuorinated graphite to explore the modification mechanism. The chemical composition and microstructure of the products were characterized and analyzed before and after the reaction. As the electrochemical reaction time or heating temperature increased, the carbon-fluorine bond gradually underwent a nucleophilic reaction with KOH according to its activity, promoting the formation of F ions in the residual product and carbon-oxygen bonds in the corresponding oxidized fluorinated graphite (OFG). The electrochemical method with a bottom anode and the heating method are insufficient to allow the isolated carbon-fluorine bond to react, retaining partial carbon-fluorine bonds. When the anode is positioned on the top, electron transfer significantly accelerates the activation of the carbon-fluorine bond, which then reacts completely. According to theoretical simulation calculations, electronegative groups around the carbon-fluorine bond can effectively enhance its reactivity.
Distributive Processor Task Scheduling for the Cyber-Physical Systems– A Traveling Salesman Problem Approach
Authors: V. Padmajothi , Iqbal J. L. Mazher 
Published: March 2022

Abstract
Task scheduling is one of the critical processes in cyber-physical systems to meet real-time deadlines. In the cyber-physical system, the processor is distributed at different locations physically and collaborative computation between them may make the computation to meet the real-time deadline. This distributive processor system has various task communication delays, which need to be considered during task scheduling. This distributive processor with varying delays of communication can be considered as a traveling salesman problem. This traveling salesman problem model-based task scheduling for distributive multiprocessor is solved using a genetic algorithm. This proposed scheduling mechanism shows improved performance comparing to the traditional scheduling algorithm.
Elevation of Major Constitutive Heat Shock Proteins is Heat Shock Factor Independent and Essential for Establishment and Growth of Lgl Loss and Yorkie Gain-Mediated Tumors in Drosophila
Authors: Singh Gunjan , Chakraborty Saptomee , Lakhotia Subhash Chandra 
Published: March 2022

Abstract

Cancer cells overexpress heat shock proteins (Hsps), the major components of cellular stress response, to overcome the diversity of stresses and to survive. However, the specific roles of Hsps in initiation and establishment of cancers remain unclear. Using loss of Lgl function-mediated epithelial tumorigenesis in Drosophila , we induced tumorigenic MARCM clones of different genetic backgrounds in wing imaginal discs to examine the temporal and spatial expression and roles of major heat shock proteins in tumor growth. The constitutively expressed Hsp83, Hsc70 (heat shock cognate), Hsp60 and Hsp27 show elevated levels in all cells of the tumorigenic clone since early stages, which persists till their transformation. However, the stressinducible Hsp70 is expressd only in a few cells at later stage of established tumorous clones that show high Factin aggregation. Intriguingly, levels of heat shock factor (HSF), the master regulator of Hsps, remain unaltered in these tumorous cells and its down-regulation does not affect tumorigenic growth of lgl - clones overexpressing Yorkie, although down-regulation of Hsp83 prevents their survival and growth. Interestingly, overexpression of HSF or Hsp83 in lgl - cells makes them competitively successful in establishing tumorous clones. These results show that the major constitutively expressed Hsps, but not the stress-inducible Hsp70, are involved in early as well as late stages of epithelial tumors and their elevated expression in lgl - clones co-overexpressing Yorkie is independent of HSF.
Selective Multimedia Data Encryption Based on Discrete Hyperchaotic S-Box Generation with Motion-Based Fragmentation
Authors: G Megala , P Swarnalatha 
Published: March 2022

Abstract
Cloud computing has become the significant technology with rapid popularization offering many services with highly effective computation and large scale storage solutions. As the multimedia files are growing explosively, securely storing it for authorized access is the major task. Concentrating on video file storage in public cloud environment, security and privacy of those outsourced videos are major concerns. The semantic elements of the HEVC videos are extracted on the horizontal and vertical video fragments and Selective Encryption on those semantic elements of videos are done when the data owner uploads video for cloud storage. Modified AES is the proposed selective encryption scheme to protect the video by encrypting each fragments in cipher feedback (CFB) process mode. A dynamic, nonlinear 2D discrete hyperchaotic mapping based S-box generation is proposed to enhance the security level by providing good confusion, diffusion and scrambling. The performance analysis of generated S-box and experimental results shows that the proposed selective encryption highly resists attacks and encrypts with reduced time complexity.
G-CSF Upregulates the Expression of Aquaporin-9 through CEBPB to Enhance the Cytotoxic Activity of Arsenic Trioxide to Acute Myeloid Leukemia Cells
Authors: Fu Wanbin , Zhu Gelan , Xu Lan , Liu Jia , Han Xiaofeng , Wang Junying , Wang Xinpeng , Hou Jian , Zhao Huanbin , Zhong Hua 
Published: March 2022

Abstract
Background: Arsenic trioxide (ATO) is widely used for acute promyelocytic leukemia (APL) treatment, but it can’t show satisfactory efficacy in non-APL leukemia. Aquaporin-9 (AQP9), a transmembrane transporter, is required for ATO uptake. Acute myeloid leukemia (AML) other than APL cells express relatively lower levels of AQP9, which limits the cytotoxic activity of ATO in those types of leukemia cells. Recently, we found that granulocyte colony stimulating factor (G-CSF) can upregulate the expression of AQP9. We hypothesized that the combination of G-CSF with ATO may enhance the antitumor effect of ATO in non-APL leukemia cells. We also aimed to elucidate the underlying mechanisms by which G-CSF upregulates the expression of AQP9.Methods: AML cell lines including THP-1 and HL-60 were pretreated with or without G-CSF (100ng/ml) for 24 hours, followed by the treatment with ATO (2μM) for 48 hours. Cell morphology was observed under microscope after Wright-Giemsa staining. Flow cytometry was performed to evaluate the cell apoptosis levels. The intracellular concentrations of ATO were determined by the atomic fluorescence spectrometry. The mRNA and protein expression were respectively measured by quantitative reverse transcription PCR (RT-qRCR) and western blotting. Target genes were knocked down by transfection with small interfering RNA (siRNA), or overexpressed by transfection with overexpression plasmids. The cell line derived xenograft mouse model was established to confirm the results of the in vitro experiments.Results: Compared with using ATO alone, the combination of ATO with G-CSF induced apoptosis in AML cells more dramatically. G-CSF upregulated the expression of AQP9 and enhanced the intracellular concentrations of ATO in AML cells. When AQP9 was overexpressed, it markedly enhanced the cytotoxic activity of ATO. On the other hand, when AQP9 was knocked down, it dramatically attenuated the combinational effect. Moreover, we found that the upregulation of AQP9 by G-CSF depends on the transcription factor CCAAT enhancer binding protein beta (CEBPB). We also demonstrated that ATO and G-CSF combination significantly inhibited the tumor growth in xenograft mouse model.Conclusions: ATO and G-CSF combination may be a potential therapeutic strategy for AML patients.