bluuebunny/crossref_metadata_2025_split huggingface dataset:
Andrographolide attenuates RSV-induced inflammation by suppressing apoptosis and promoting pyroptosis after respiratory syncytial virus infection in vitro
Authors: Che Siyi , Xie Xiaohong , Lin Jilei , Liu Ying , Xie Jun , Liu Enmei 
Published: March 2022

Abstract

Respiratory syncytial virus (RSV), the most common viral pathogen causing acute lower respiratory tract infection among children under five years old worldwide, still lacks specific therapeutic drugs. Andrographolide has been found efficacious in various virus infections, however, its effects on RSV infection remains unknown. Herein, in this study we quantified RSV virus load, IL-6 and IL-8 in A549 cell supernatant, and found andrographolide decreased the viral load and attenuated RSV-induced inflammation. Then, using online databases, we discovered 25 potential targets of andrographolide in the treatment of RSV-infected airway epithelial cells. Subsequently, GO and KEGG enrichment analysis led to the identification of CASP1, CCL5, JAK2, STAT1 as significant players. We verified the mRNA expression change of the potential target genes, which showed that andrographolide noticeably suppressed the increase of CASP1, CCL5, JAK2 and STAT1 post RSV infection. IL-1β, which is downstream of CASP1, was also up-regulated after RSV infection and down-regulated by andrographolide. Furthermore, we conducted Annexin V-FITC/PI apoptosis assay and Western blotting to determine whether andrographolide has an impact on the death pattern of RSV-infected cells. Interestingly, RSV infection decreased the protein levels of caspase-1, cleaved caspase-1, cleaved IL-1β, N terminal of GSDMD and Bcl-2, while andrographolide elevated them. These results suggested that andrographolide might attenuate RSV-induced inflammation by suppressing apoptosis and promoting pyroptosis of infected epithelial cells and thus inducing effective viral clearance.
Effect of cryogenic treatment on corrosion resistance of AZ61 magnesium alloy welded joint
Authors: Gong Xiaoyuan , Liu Jinming , Li Junqi , Xiao Fengqing 
Published: March 2022

Abstract
The welding test of 7mm thick AZ61 magnesium alloy plate was carried out by MIG welding; the joint was cryogenically treated at -180℃, and the holding time was 4h, 8h and 12h respectively; Weight loss method and electrochemical method were used to test the influence of cryogenic treatment on the corrosion resistance of welded joints before and after cryogenic treatment, and the corrosion morphology of specimens was observed by SEM. The test results show that the corrosion resistance of the joint sample reaches the best when the cryogenic treatment temperature is -180℃ and the holding time is 8h.
Association Analysis Of IKZF4 Gene With Alopecia Areata In Chinese Han Population
Authors: Miao Ying , Hu Ruiming , Sheng Youyu , Qi Sisi , Yang Qinping 
Published: March 2022

Abstract
Background: The IKZF4 gene encodes Eos, a zinc finger transcription factor that belongs to the Ikarosfamily. High expression of Eos on Treg cells is important for the suppression of autoimmune responses and immune homeostasis. It has been suggested that the SNP in IKZF4 may influence the pathogenesis of AA.Aims and Objectives: The purpose of this study was to explore the relationship between IKZF4 polymorphism and AA in Chinese Han population.Materials and Methods: In this study, we examined 459 patients and 434 controls using HRM analysis and direct sequencing to assess.the rs1701704 polymorphism.. Results: The prevalence of the C/C, A/C and A/A genotypes was 7.4%, 37.5% and 55.1%, respectively. There were significant differences in genotype distribution and allele frequencies between AA and control group (p<0.0001). The frequency of C allele in AA group was significantly higher(p<0.0001), and the frequencies of C allele and C/C genotype in patients with family history were higher (p<0.0001;p=0.001;).Conclusions: The rs1701704 SNP of IKZF4 may be a genetic marker for assessing the risk of AA in Chinese Han population.
Montreal Cognitive Assessment of cognitive dysfunction after basal ganglia stroke
Authors: Ye Baoye , Wei Dingqun , Pan Lin 
Published: March 2022

Abstract
Objective

The Montreal Cognitive Assessment (MoCA) was used to evaluate cognitive dysfunction after basal ganglia stroke, and factors affecting total MoCA score were examined.

Methods

Data were retrospectively analyzed for 30 patients with basal ganglia intracerebral hemorrhage or basal ganglia cerebral infarction, who were admitted to The Second Affiliated

Hospital of Fujian Traditional Medical University (Fujian, China) from January 2017 to March 2020. Cognitive impairment was assessed using the MoCA, and potential correlations were explored between clinicodemographic characteristics (sex, age, stroke location and etiology) and

MoCA dimensions or total MoCA score.

Results

Univariate linear regression showed that the total MoCA score was significantly associated with sex, age, executive function, naming, attention, abstract generalization ability, memory ability, and visuospatial orientation. However, multivariate linear regression identified only executive function, naming, attention, memory ability, and visuospatial orientation as significantly associated with the total MoCA score.

Conclusions

We showed that the MoCA test can be used for patients with basal ganglia stroke. The total MoCA score of basal ganglia stroke was significantly associated with impairments in executive function, naming, attention, memory ability, and visuospatial orientation.
Hydrazone based materials; Quantum chemical studies, Spectroscopic Properties and A Comparable Experimental-Theoretical investigation.
Authors: sakr mahmoud , Sherbiny Farag F. , El-Etrawy Abd-Allah Sh. 
Published: March 2022

Abstract
Due to numerous pharmaceutical and biological activities hydrazone (TC) based materials, it was important to investigate quantum chemical studies such as Density functional theory (DFT) calculations, natural bond orbital (NBO) analysis, molecular electrostatic potential (MESP), and local reactivity usage Fukui function for six TC derivatives compounds. DFT, NBO, MESP, and local reactivity calculations were obtained via utilizing CAM-Becke's three-parameter functional and Leee Yange Parr (CAM-B3LYP) functional and 6-311G++(2d, 2p) basis set. Optimized molecular structures for all studied compounds were obtained usage the DFT/CAM-B3LYP/6-311G++(2d, 2p) method. In addition, the highest occupied molecular orbital (HOMO), lowest unoccupied molecular orbital (LUMO), energy gap (Eg), light harvest efficiency (LHE), and open-circuit voltage (Voc) of all studied MSs are calculated and illustrated. These properties indicate that these molecular modeling structures as good candidates for utilization in organic DSSCs. The calculated spectroscopic investigations of hydrazine derivatives have been obtained by applying TD/B3LYP/6-311G++(2d, 2p) method. the calculated UV-Vis absorption spectra for molecular structures under study show nice correlations with experimental spectra.
Systematic Pan-cancer Analysis on the Expression and Role of RCC1/SNHG3/SNHG12
Authors: Zhang Yan , Hu Kai , Yu Huomei , Liu Shiyan , Liao Deyu 
Published: March 2022

Abstract
Regulator of chromatin condensation 1 (RCC1) is the major guanine nucleotide exchange factor of RAN GTPase, which plays a key role in various biological processes such as cell cycle and DNA damage repair. Small nucleolar RNA host gene 3 (SNHG3) and SNHG12 are long-stranded non-coding RNAs (lncRNAs) and are located on chromatin very close to the sequence of RCC1. Many studies have shown that they are aberrantly expressed in tumor tissues and can affect the proliferation and viability of cancer cells. Although the effects of RCC1/SNHG3/SNHG12 on cellular activity have been reported, respectively, their overall analysis on the pan-cancer level has not been performed. Here, we performed a comprehensive analysis of RCC1/SNHG3/SNHG12 in 33 cancers through the Cancer Genome Atlas and Gene Expression Database. The results showed that RCC1/SNHG3/SNHG12 were highly expressed in a variety of tumor tissues compared to normal tissues. The expression of RCC1/SNHG3/SNHG12 in BRCA, LGG and LIHC was associated with TP53 mutations. In addition, RCC1/SNHG3/SNHG12 expression was closely associated with the prognosis of patients with multiple tumors. Immunocorrelation analysis indicated that RCC1/SNHG3/SNHG12 showed a correlation with multiple immune cell infiltration. The results of enrichment analysis suggested that RCC1/SNHG3/SNHG12 was involved in the regulation of cell cycle, apoptosis and other pathways. We found that these effects were mainly mediated by RCC1, while the trend of SNHG3/SNHG12 regulation was also consistent with RCC1. We refer to this phenomenon as "homodomain effect". These findings provide new and comprehensive insights into the role of RCC1/SNHG3/SNHG12 in tumor development and show their potential as clinical monitoring and therapy.
Primordial germ cells identification and traceability during the initial development of the Siluriformes fish Pseudopimelodus mangurus.
Authors: Monzani Paulo Sérgio , Shiguemoto Gustavo Fonseca , Coelho Geovanna Carla Zacheo , López Lucia Suárez , Pessoa Giselle Pessanha , Santos Silvio Carlos Alves dos , Senhorini José Augusto , Yasui George Shigueki 
Published: March 2022

Abstract
Primordial germ cells (PGCs) are responsible for the generation of all germ cells, and therefore, they are essential targets to be used as a tool for the production of germline chimera. The labeling and route of PGCs were evaluated during the initial embryonic development of Pseudopimelodus mangurus, using whole-mount in situ hybridization (WISH) and mRNA microinjection in zygotes. The WISH method was synthesized a specific RNA antisense probe using part of the coding region from P. mangurus nanos3 cDNA. The RNA microinjection was performed using the GFP gene reporter regulated by translation regulatory P. mangurus bucky ball and nanos3 3’UTR sequences, which are germline-specific markers used to describe in vivo migration of PGCs. The nanos3 and bucky ball gene expressions were evaluated in tissues for male and female adults and initial development phases and larvae from first to seventh days post-hatching. The results from the WISH technique appointed to the origin of PGCs in P. mangurus from the aggregations of nanos3 mRNA in the cleavage grooves and the signals obtained of nanos3 probes corresponded topographically to the migratory patterns of the PGC reported for other fish species. The microinjection of GFP-nanos3 3'UTR mRNA in the zygotes of P. mangurus was not successful for PGC labeling, but it was possible to identify three embryos injected, presenting 3 to 5 evidence of possible PGC labeled in the hatching phase. The nanos3 and bucky ball gene expression was reported in the female gonads and from fertilized eggs until the blastula phase. These results provide information about the PGC migration of P. mangurus and the possible use of PGCs for the future generation of germline chimera to be applied in the conservation efforts of Neotropical Siluriformes species, contributing to the establishment of genetic banks, manipulation of organisms, and assisting in biotechnologies such as the transplantation of germ cells in fish.
Integrative approaches of DNA methylation patterns according to age, sex, and longitudinal changes
Authors: Gim Jeong-An 
Published: March 2022

Abstract
Background: In humans, age-related DNA methylation has been studied in blood, tissues, buccal swabs, and fibroblasts, and changes in DNA methylation patterns according to age and sex have been detected. To date, approximately 137,000 samples have been analyzed from 14,000 studies and the infor-mation has been uploaded to the NCBI GEO database.Methods: A correlation between age and methylation level and longitudinal changes in methylation levels was revealed in both sexes. Here, 20 public datasets derived from whole blood were analyzed using the Illumina BeadChip.Results: Of the 20 datasets, nine were from a longitudinal study. All data had age and sex as common variables. Comprehensive details of age-, sex-, and longitudinal change-based DNA methylation levels in the whole blood sample were elucidated in this study. ELOVL2 and FHL2 showed the maximum correlation between age and DNA methylation. The methylation patterns of genes related to mental health differed according to age.Conclusions: Based on 20 public DNA methylation datasets, methylation levels according to age and longitu-dinal changes by sex were identified and visualized using an integrated approach. The results highlight the molecular mechanisms underlying the association of sex and biological age with changes in DNA methylation, and the importance of optimal genomic information management.
Sexual Segregation in the Foraging Distribution, Behaviour, and Trophic Niche of the Endemic Boyd’s Shearwater (Puffinus lherminieri Boydi)
Authors: Santos Ivo dos , Ramos Jaime A. , Ceia Filipe R. , Rodrigues Isabel , Almeida Nathalie , Antunes Stefan , Carreiro Ana R. , Matos Diana M. , Lopes Ricardo J. , Geraldes Pedro , Paiva Vítor H. 
Published: March 2022

Abstract
Studies on sex-specific segregation in foraging and trophic niche have been focused on large and dimorphic seabirds, with less information on small monomorphic species. Here, we used mini-GPS loggers, habitat suitability models, and stable isotopes to assess the foraging movements, at-sea spatial distribution, and trophic ecology of male and female Boyd’s shearwaters Puffinus lherminieri boydi in Raso Islet (16°36’ N, 24°35’ W), Cabo Verde, during the breeding seasons of 2018–2019. The existence of sexual foraging segregation was tested in short and long foraging trips. Females engaged on longer foraging trips, travelling towards more distant and northward regions from the colony when compared to males, especially during long foraging excursions. Spatial overlap within and between sexes was generally low, indicating a sex-specific pattern in the foraging behaviour and spatial distribution of adult breeders. Habitat suitability models revealed a higher importance for chlorophyll a concentration in explaining females’ at-sea distribution during long foraging trips when compared to males, although the most important predictors in explaining adults’ distribution were sea surface temperature and height for short and long excursions, respectively. Stable isotope analysis revealed that both sexes occupied similar isotopic niches and stable isotope mixing models revealed no diet differences. This indicates that Boyd’s shearwaters segregate at the spatial level while foraging but rely on similar food resources. Our results suggest that female-biased nutritional requirements at the onset of chick-rearing may be driving sexual foraging segregation in this population, which depends upon resources from a rather oligotrophic environment.
Characterization and Management of Facial Angiofibroma Related to Tuberous Sclerosis Complex in the United States: Retrospective Analysis of the Natural History Database
Authors: Boggarapu Sreedevi , Roberds Steven L , Nakagawa Jo Anne , Beresford Eric 
Published: March 2022

Abstract
Background: Facial angiofibroma is the most predominant cutaneous manifestation of tuberous sclerosis complex (TSC), a rare autosomal dominant genetic disorder impacting the mechanistic target of rapamycin (mTOR). Facial angiofibroma can bleed spontaneously, impair eyesight, and cause aesthetic disfiguration causing psychological and social stress. To date, there is little or no evidence on the demographics, and other TSC features associated with facial angiofibroma or the use of mTOR inhibitor for the management of facial angiofibroma. This is a retrospective study of TSC Alliance’s Natural History Database aimed to characterize facial angiofibroma and to evaluate features associated with a higher risk of facial angiofibroma or the use of topical mTOR inhibitors for management of facial angiofibroma. Data in the NHD was obtained from 18 clinical sites in the US since 2006.Results: Of the 2240 patients, 2088 patients were enrolled in the US and data from 2057 patients were included in this analysis. Patient features associated with a higher risk of facial angiofibroma or the use of topical mTOR inhibitors. Facial angiofibroma was noted in 1329 (64.4%) patients with TSC. Patients with facial angiofibroma were older on average. TSC2 mutation was more common, and the burden of other TSC-related manifestations was significantly higher in patients with facial angiofibroma. The 6–17 and the 18–45 -year-old age groups, TSC2 mutation, angiomyolipoma, and renal cysts were significantly associated with a higher risk of facial angiofibroma. Topical mTOR inhibitor use was noted for 329 (24.8%) patients with facial angiofibroma. Being white and presence of angiomyolipoma were significantly associated with higher use of a topical mTOR inhibitor.CONCLUSIONS: Patients with facial angiofibroma were older and more commonly had a TSC2 mutation. The higher burden of TSC-related manifestations in patients with facial angiofibroma illustrates the importance of accurate diagnosis by dermatologists and referral to a comprehensive multi-disciplinary TSC Clinic for surveillance and management of other TSC manifestations. About one-fourth of patients with facial angiofibroma used a topical mTOR inhibitor.
Network Pharmacology Study to Reveal Underlying Mechanisms, Targets, and Bioactives of Aralia Cordata Against Obesity
Authors: Oh Ki Kwang 
Published: March 2022

Abstract
Aralia cordata (AC) has been used as anti-obesity herbal plants by Chinese, Japanese, and Korean, but its active chemical constituents, mechanism(s), and targets have not been documented completely. We aimed to investigate significant phytochemicals, pathways, and targets of AC against obesity via network pharmacology. The phytochemicals from AC were identified by Gas Chromatography Mass Spectrum (GC-MS) and were screened subsequently by Lipinski’s rule. The compound-target relationships were retrieved by analyzing SwissTargetPrediction (STP), SEA search server. Then, obesity-related targets were identified by public bioinformatics and final overlapping targets were selected by Ven diagram. Next, we constructed and visualized protein-to-protein interaction (PPI) networks, bubble chart, and pathways-targets-compounds (PTC) networks by RPackage. Furthermore, we utilized the Autodock Tools to perform molecular docking test (MDT) on the bioactives and key targets to validate the network pharmacological results. We confirmed a total of 43 compounds from AC via GC-MS and 40 final targets regarding obesity. The PPI networks analysis revealed IL6 as a key target, and a bubble chart showed that inactivation of Insulin resistance might be the uppermost pathway for anti-obesity. We identified that the AC phytochemicals was contributed to synergistic effects (multi-pathway, muti-target) to alleviate obesity through PTC analysis. We conducted MDT to verify the most significant compound on a key target (IL6), thereby, confirmed Andrographolide as a key compound of AC on obesity. Overall, we elucidated a key pathway, target, and bioactive of AC against obesity, suggesting significant pharmacological basis for further clinical trials.
High Level of Noise Affects the Consumption of Anesthetic Agents during Total Intravenous Anesthesia (TIVA) and the Satisfaction of Patient and Surgeon
Authors: Mizrak Ayse 
Published: March 2022

Abstract
Objective: The purpose of this study is to investigate the effects of high noise level on propofol and remifentanyl consumption in patients who undergo cholecystectomy surgery under total intravenous anaesthesia (TIVA) during operation and on the satisfaction of patients and physicians. Subjects and Methods: 90 patients who would undergo cholecystectomy surgery were randomized in 3 groups in this study planned as a double blind, prospective, randomized clinical trial. Propofol and remifentanyl infusion was started to obtain BIS values between 40-60 for all patients. The patients in Group N (n=30) were subjected to noise of normal operating room. The patients in Group S (n=30) were subjected to noise between 80-85 Db with headphone and for patients in Group Q subjection to noise of the operating room was prevented by covering the ears with headphones (n=30). Intraoperative total remifentanyl and propofol consumption of the patients, postoperative patient and physician satisfaction, postoperative side effects were recorded. Results: Total remifentanyl and propofol used during surgery was significantly lower in Group Q than in Group N and Group S (p=0,0001, p=0,04). Postoperative patient satisfaction in Group Q was statistically higher than in Group N and Group S (p=0.001). Surgeon satisfaction in Group Q was also higher than in Group N and Group S (p=0.01). Conclusion: High noise level for patients who undergo cholecystectomy surgery under TIVA increases total remifentanyl and propofol consumption during operation. In addition, it decreases patient and surgeon satisfaction.
Genome-Wide Identification, Characterization, and Expression Profiling Analysis of SPL Gene Family During The Inflorescence Development in Trifolium Repens
Authors: Ma Jieyu , Nie Gang , Yang Zhongfu , Ma Sainan , Fan Jinwan , Hu Ruchang , Wu Feifei , Zhang Xinquan 
Published: March 2022

Abstract

Trifolium repens is the most widely cultivated perennial legume forage in temperate region around the world. It has rich nutritional value and good palatability, seasonal complementarity with grasses, and can improve the feed intake and digestibility of livestock. However, flowering time and inflorescence development directly affects the quality and yield of T. repens, as well as seed production. Squa promoter binding protein–like (SPL) gene family is a plant specific transcription factor family, which has been proved to play a critical role in regulating plant formation time and development of flowers. In this study, a total of 37 TrSPL genes were identified from the whole genome of T. repens and were divided into 9 clades based on phylogenetic tree. The conserved motif of squamosa promoter binding protein (SBP) contains two zinc finger structures and one NLS structure. Gene structure analysis showed that all TrSPL genes contained SBP domain, while ankyrin repeat region was just distributed in part of genes. 37 TrSPL genes were relatively dispersedly distributed on 16 chromosomes, and 5 pairs of segmental repeat genes were found, which indicated that segmental duplication was the main way of gene expansion. Furthermore, the gene expression profiling showed that TrSPL11, TrSPL13, TrSPL22 and TrSPL26 were highly expressed only in the early stage of inflorescence development, while TrSPL1 and TrSPL6 are highly expressed only in the mature inflorescence. Significantly, the expression of TrSPL4 and TrSPL12 increased gradually with the development of inflorescences. The results of this study will provide valuable clues for candidate gene selection and elucidating the molecular mechanism of T. repens flowering regulation.