bluuebunny/crossref_metadata_2025_split huggingface dataset:
NEW VITAMIN-MINERAL PREPARATION FOR PREVENTION OF OBSTETRIC PATHOLOGY OF ANIMALS «BIOTAN 3Z»
Authors: Katsaraba O. A. , Sachuk R. M. , Stravsky Ya. S. , Kostyshyn L.-M. Y. 
Published: January 2022

The article presents the vitamin and mineral preparation «Biotan 3Z" which includes micro- and macroelements in the form of water-soluble salts, namely Calcium, Phosphorus, Magnesium, Iron, Copper Manganese, Copper, Cobalt and Manganese. It also contains many vitamins (aspartic acid, glutamic acid, vitamin A, E, B1, PP) and essential amino acids - rich in methionine, lysine, arginine, histidine, tyrosine, phenylalanine, isoleucine, leucine, valine, cystine, tryptophan, glycine, proline, threonine and serine, which are necessary for the normal functioning of the body. Due to this veterinary drug the level of diseases of domestic animals decreases, their productive qualities increase. This drug accelerates the growth of animals. Vitamin and mineral preparation «Biotan 3Z" reduces the incidence and mortality of animals. Prevents diseases that originate from changes in housing conditions or occur against the background of metabolism or lack of vitamins and minerals.
When using this drug in bitches, the biochemical and morphological parameters of the blood of these animals were analyzed. Significant changes in blood parameters such as erythrocytes, hemoglobin, hematocrit, total protein, before and after administration of the drug in various forms of manifestation have not been established. There was only a slight increase in erythrocytes from 6,6±0,2 thousand / ml to 7,8±0,4 thousand/ml, as well as a slight increase in hemoglobin from 120±3,8 g/l to 146±6,8 g/l. Vitamins and minerals contained in the fed drug had a positive effect on metabolism in the mother and fetus, as evidenced by changes in blood creatinine, which at 14 weeks before delivery was 127,6 μmol/l and on the first day of delivery in the experimental group increased to 135.8 μmol/l.
The addition of a vitamin-mineral preparation to the main diet in some way affected the metabolism and biochemical composition of the blood of the bitches of the experimental group. It was expressed by an increase in the physiological limits of glucose, total protein, albumin, creatinine, cholesterol, total calcium, inorganic phosphorus and a decrease in globulins, bilirubin and AST. The concentration of urea, triglycerides and LF activity almost did not change.
Feeding of vitamin-mineral preparation «Biotan 3Z» to bitches before childbirth affected the content in the blood of experimental animals of total calcium and inorganic phosphorus, the concentration of which increased by 0,2 mmol / l compared to baseline, and 0,2 and 0,3 mmol/l – with the control group after the experiment. Vitamin and mineral preparation «Biotan 3Z» is of interest for veterinary medicine and therefore it is advisable to continue its further clinical study.
INFLUENCE OF MORPHOLOGICAL INDICATORS OF SEEDS OF RAMS OF DIFFERENT BREEDS ON THE QUANTITY AND QUALITY OF SPERM PRODUCTS
Authors: Kitaeva A. P. , Mamedova V. M. 
Published: January 2022

Assessment of sperm quality is an important part of the technological process. Fertility of uteruses and bright depends to a large extent on quality of sperm. Before fertilization, you can get an idea of ​​the quality of sperm, to determine some indicators (volume, activity, density) that characterize certain properties.
The main method of research in the production environment is the visual assessment of sperm by density and motility, concentration and resistance, and the percentage of live sperm.
The testes are a pair of ovoid tubular glands that are located in the testis sac, the outer layer of which is called the scrotum. Each testicle is covered with three shells: protein (covers the surface of the testicle), a special vagina and a common vagina. At the head of the testis, where the head of the appendix departs, the protein shell grows deep into the testis, forming the mediastinum of the testis, and divides the parenchyma into many lobes or chambers. Inside the chambers are thin tubes (0.1-0.2 mm) - twisted tubules of the testis, covered with delicate connective tissue and interstitial cells. The quality and quantity of sperm production of rams of different breeds (assaf, dorper, merinoladshaf) were studied. It was found that sheep of the dorper breed of meat productivity in terms of ejaculate volume exceed the indicators of sheep of the breed assaf dairy-meat direction by 0.11 ml or 20%, and compared with sheep of meat-wool direction of productivity of the breed merinolandshaf respectively by 0.1 ml or 17.8%. According to the semen density of sheep of the assaf breed, the indicators of dorper and merinoladshaf were compared. The average density of semen of sheep of the assaf breed is exceeded in comparison with the dorper breed by 2 times and that of the merinoladshaf by 4 times. Dorper and merinoladshaf had liquid semen, which was estimated at 2 points. The sperm of all breeding rams had a rectilinear oscillating motion. High-quality semen was found in rams of all breeds and was evaluated at 9 points in rams of the assaf breed, and in rams of the dorper and merinoladshaf breeds - at 8 points.
CLINICAL TRIALS OF VETASEPTOL FOR THE TREATMENT OF CALVES WITH SIGNS OF ACUTE INTESTINAL INFECTION
Authors: Lisova N. , Zhyla M. , Sobodoch O. , Myhaljuk O. 
Published: January 2022

The aim of the present study was to investigate of the therapeutic efficacy of the two- components antimicrobial drug. The drug contains two antimicrobial components that act synergistically. Acute intestinal infection of calves was treated. The drug was given with food. The dose and method of administration were determined by the manufacturer. Before use, the sensitivity of pathogenic microorganisms to the active components of the drug was determined. During the experiment, constant monitoring of the clinical condition and physiological parameters of the organism of experimental animals was performed.
Evaluation of hematological and biochemical parameters of the body was performed according to recognized methods. Therapeutic efficacy was compared with data obtained using the reference drug. The obtained data were processed statistically. Based on the obtained data, conclusions were made about the effectiveness of treatment and the effect of the drug on the body of young cattle.
According to the assessment of the clinical condition of the animals and the results of laboratory studies, it was found that the tolerability of the study drug for the treatment of intestinal infections in young animals was good. During the experimental period and two weeks after the use of drugs, no side effects were detected and no changes in the behavior of calves were observed. The therapeutic efficacy of the study drug was 100 %, and the recovery period was 5 days. Comparative analysis of hematological parameters of calves showed a significant decrease in the percentage of rod-shaped neutrophils in the leukogram of sick animals after antibiotic therapy. Normalization of the leukogram indicated the cessation of inflammatory processes and improvement of the physiological condition of animals. Analysis of the influence of drugs on biochemical indices in experimental calves showed no changes during the observation period. Negative impact on liver indicators under administration weren't found. Laboratory studies have not revealed nephrototoxic effects of antimicrobial drugs. Confirmed the safety and therapeutic efficacy of the two antimicrobial components drug.
DEVELOPMENT OF THE METHOD FOR QUANTITATIVE DETERMINATION OF NEOMYCIN SULPHATE IN VETERINARY PREPARATIONS WITH PRECOLUMN DERIVATIZATION AND FLUORESCENT DETECTION
Authors: Ostapiv R. D. , Humeniuk S. L. , Tkachenko V. І. , Chipak S. Yu. 
Published: January 2022

The aim of work was to develop a method for the identification and quantification of neomycin sulfate in intracisternal veterinary preparations. The method was developed and validated by: selectivity, linearity and suitability parameters of the chromatographic system. A four-component intracisternal drug with a 25 mg/g neomycin content was used as an object sample for method development. The standard sample and the test sample were dissolved in purified water to a concentration of 100 μg/ml, followed by derivatization with 9-fluorenylmethoxycarbonyl chloride at room temperature. The total uncertainty of the analysis was 1.62 %, which is within the limits recommended in SFU 2.0. The samples were separated on a Dionex Ultimate 3000 chromatograph equipped with an Acclaim C18 150 × 4.6.5 μm chromatographic column. The mobile phase was a mixture of acetonitrile and water in a volume ratio of 95:5. Neomycin sulfate was detected fluorescently at wavelengths λex - 254 nm, λem - 303 nm.
Under the above conditions, it was possible to completely separate the two isoforms of neomycin (retention time of chromatographic peaks - 6.57 min and 6.98 min), 9-fluorenylmethoxycarbonyl chloride and other components of the study drug (retention time of the corresponding chromatographic peaks - 2-5 min). In this case, the parameters of the suitability of the chromatographic system did not exceed the limits specified in the recommendations of the USA Food and Drug Association. For peaks of neomycin sulfate, the efficiency of the chromatographic system was 7700–7950 theoretical plates. The relative standard deviation (RSD) for the peak areas of the active substance was ± 0.33%, and the coefficient of separation of the peaks (RS) of neomycin sulfate from the peaks of the derivatization reagent and other components of the drug was 11.9. The coefficient of peak symmetry was 1.18. The calibration curves were linear in the recommended SFU 2.0 range (80–120% of the nominal concentration of the corresponding active substance). The coefficient of linearity (R2) for the sum of neomycin sulfate peak areas was – 0.9991.
SLAUGHTER INDEXES, MORPHOLOGICAL COMPOSITION OF CARCASES AND MUSCLE TISSUE QUALITY OF REARING PIGS UNDER FEED ADDITIVE "ACTIVO" SUPPLEMENTATION
Authors: Prudius T. Y. , Hutsol A. V. , Hutsol N. V. 
Published: January 2022

Science and practice have established that properly organized pig feeding, allows to increase animal usage of nutrients from the diet, which positively influences the growth rate of pig rearers and improves their meat quality.
Supplementation of rearing pig diets with the feed additive "Aсtivo" has no negative impact on feed intake and positively influences the slaughter indexes, the morphological carcasses content and the internal organs’ mass. The pigs slaughtering indicators of the trial group were better, which proves the efficiency of the feed additive "Active" supplementation. The slaughtered mass of animals from the trial group was improved by 3.32 kg, comparing to the control group. Similarly, the carcass mass of the animals of the trial group increased on 6.52 kg comparing to control. This resulted in slaughter yield improvement on 6,59 % in trial group.
There is no significant difference in the mass of by-products, but there is a tendency to some increase in the trial group.
Supplementation of young pigs with the feed additive "Activo" has a negligible effect on the carcasses morphological composition and causes a tendency to increase the meat and fat yield.
Feeding rearing pigs of "Activo" has no probable effect on the change of physico-chemical indicators of meat quality. However, the actual figures have some differences in individual indicators within the statistical deviations. Laboratory studies of the Longissimus dorsi muscle showed that there was no significant difference between trial and control groups in water holding capacity of muscle tissue. Also, there is no significant difference in terms of meat marbling and color intensity.
EPIZOOTOLOGICAL FEATURES CORONAVIRUS INFECTION IN CATS
Authors: Radzіkhovskyі N. , Dyshkant О. , Tolokevich O. , Moshkivsky V. 
Published: January 2022

The exact number of cats living on our planet is unknown, but there are reports with reference to studies conducted by French scientists from the University of Lyon (France) in 400 million domestic cats, most of which live in the United States and Brazil (93 and about 100 million. in accordance). However, according to researchers, the first place in the world in terms of the number of cats per capita is Australia (ratio of 9:10), and Ukraine is in the top 10 countries with the largest number of domestic cats.
In the mid-90s of the twentieth century, Ukraine faced a new infectious disease that affects different species of the feline family - infectious feline peritonitis (IPC), the causative agent of which is a virus of the family Coronaviridae. Animal coronaviruses have been a problem for more than 50 years, however, given their variability and large diversity, the study of this group of viruses continues today.
The article presents data on the epizootological features of coronavirus infection in cats for the period 2019 - 2020 in veterinary clinics in Zhytomyr and Kyiv. During a certain period of time, during the experiment, 115 samples were taken for the study, of which 95 animals were detected with the virus of the family Coronaviridae.
The paper highlights the results of the study of age and breed predisposition. It has been found that 2-6 month old kittens are most prone, especially breeds such as British, Persian and Scottish. Indicators of seasonality of manifestation, and also dynamics of morbidity of cats with a coronavirus infection are resulted. In the spring and summer, the peak incidence of the studied disease was noted.
According to the results of the epizootic analysis, a nosological profile of infectious diseases in cats was formed, which is represented by 8 infections, and the most frequently registered diseases are caused by viruses of the families Herpesviridae Caliciviridae and Parvoviridae.
THE PROLONGED INFLUENCE OF PROBIOTIC PROTECTION OF MUCOSY COWS BEFORE INSEMINATION ON THE EXPRESSION OF THE PRODUCTIVE POTENTIAL OF THEIR FIRSTBORN DAUGHTERS
Authors: Sidashova S. A. , Avdosyevа I. K. , Chornyi V. O. , Chajkovska O. I. , Popova I. M. , Roman L. G. 
Published: January 2022

The aim of our study was to determine the prolonged delayed action of probiotic feed protection of the intestinal mucosa of cows in the period before fertilization for the manifestation of the productive potential of their daughters during the first lactation.
Analytical work was carried out during the 2016-2019 economic years, the experimental part of the study was carried out on the basis of a dairy complex-breeder of Ukrainian red dairy cattle (700 dairy heads), located in Odessa region.
The results of a scientific and industrial study of a number of indicators of productivity of replacement heifers / heifers / heifers of the Ukrainian red dairy breed showed that the use of the feed probiotic preparation "Veterinary Multibacterin Bs + La suspension - feed mixture" together with the main diet of mothers before artificial insemination gave a positive prolonged effect on the manifestation of the productive potential of their half-siblings. For standard lactation, first-calf heifers in the experiment had a higher milk productivity by 6.99 % (respectively: 8121 and 7540 kg per head, P <0.05). At the same time, the first-calf heifers of the experimental group had a more physiological term for the interstitial period, namely: 425.39 days, which was 56.28 days shorter than in the control and contributed to the profitability of production.
It has been experimentally established that probiotic protection of the intestinal mucosa of mothers cows has a positive effect on the quality of the offspring, which forms the next generation of the breeding herd.
DETERMINATION OF VIRULICIDAL PROPERTIES OF EXPERIMENTAL SUKHODEZ DISINFECTANT
Authors: Fotina Т. , Slaston D. 
Published: January 2022

The paper considers the determination of the effectiveness of the virucidal concentration of the new powdered disinfectant «Sukhodez» in relation to DNA-containing and RNA-containing viruses, namely avian smallpox viruses and duck hepatitis.
The research was conducted in the laboratory of virology of the Department of Pat anatomy, Virology and Poultry Diseases. To determine the effectiveness of virucidal concentrations of the tool against smallpox virus - DNA-containing, cultured on the chorionic-allantoic membrane in 10-12-day-old chicken embryos and hepatitis virus ducklings - RNA-containing, used a suspension of virus-containing material, which virus on primary cultures of duck embryo cells.
The aim of the work was to investigate a new powdered disinfectant «Sukhodez», namely its virucidal properties.
The study was conducted in two stages. The first determined the effect of the drug on RNA-containing virus (Avihepatovirus A), and the second stage of research on DNA-containing virus (Fowlox virus).
Thus, at the first stage of the research, the effect of «Sukhodez» disinfectant on duckling hepatitis virus was determined at a dose of 25, 50, 75 and 100 g of the product per m2 with an exposure of 15, 30 and 60 minutes and the control was treated with sterile water. Thus, at a dosage of 25 g / m2, the product shows a disinfectant ability of 46.3% in 15 minutes, and at an exposure of 30 minutes. completely inactivates the virus; also studies have shown that at a dosage of 75 and 100 g./m2 at an exposure of 15 minutes complete inactivation of the virus took place on the washes.
In the second stage of the study, the effect of the virucidal action of «Sukhodez» on the DNA-containing avian smallpox virus, which was cultured on the chorionic allantoic membrane of 10-12-day-old chicken embryos, was determined. During the study, it was found that at a concentration of 50 g / m2 disinfectant «Sukhodez» after 15 minutes inactivated the virus by 98.3%. However, after 30 minutes, the effectiveness of the disinfectant increased to 100%. When treating surfaces at the rate of 75 and 100 g / m2 of the tool «Sukhodez» after 15 minutes there was a complete inactivation of the virus. not found.
According to our research on the virucidal action of the disinfectant, it was found that the experimental disinfectant «Sukhodez» has virucidal properties to RNA-containing and DNA-containing viruses at a rate of 50/75 g / m2 area.
EXPERIMENTAL JUSTIFICATION OF THE EFFICIENCY OF BIOLIDE DISINFECTANT FOR DISPOSAL OF BACTERIAL INFECTIONS IN CONDITIONS OF INDUSTRIAL POULTRY
Authors: Chechet O. M. , Kovalenko V. L. , Garkavenko T. O. , Gorbatyuk O. I. , Kozytska T. G. , Andriyashchuk V. O. 
Published: January 2022

The materials of the article are devoted to the study of the bactericidal action of the newly developed «Biolaid» disinfectant at the first stage to determine the possibility of its use in the poultry industry to replace expensive, often toxic and ineffective disinfectants that have now flooded the market in Ukraine.
Modern poultry farms are integrated enterprises, the structure of which consists of one continuous link with an incubator, own parent and industrial herds, production shops for processing products and feed production. One of the most important factors in the successful operation of such enterprises is quality disinfection.
The problem of developing new cheap, safe, effective disinfectants remains relevant. The problem is supplemented by questions on the study of the effects of disinfectants on the indicators of general resistance in poultry and their impact on the productivity of birds, as such information is quite limited.
Bacterial suspensions of test cultures with a concentration of 0.5–1.0 according to the McFarland standard (microbial load from 1.35×108 to 3.0×108 CFU/cm3) were prepared for the study by washing the colonies of the daily culture of microorganisms with trypton-soy agar (TCA) with sterile saline. The working dilutions of the experimental disinfectant were made according to the recommendations in the leaflet-tab, in concentrations of 0.1; 0.2; 0.25 and 0.5 % at exposures of 10, 20 and 30 minutes.
The results of studies of the newly developed «Biolaid» disinfectant showed its high efficiency, proven experimentally, because at the level of 0.25 % concentration of the working dilution of the drug at an exposure of 30 minutes. complete neutralization of test cultures of Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442 was achieved without bacteriostatic effect, which testified to the effectiveness of the drug in its action on gram-negative and gram-positive pathogenic bacteria.
Outcome Comparison of Endoscopic (Endonasal Trans-Sphenoidal) Repairs of CSS Leak vs. Transcranial Approach
Authors: Simair Ishfaque Ahmed , Ali Jawad , Khattak Abid , Qureshi Amjad , Din Tarique Salah-ud- 
Published: January 2022

Objective: To compare the effectiveness of Endoscopic (Endonasal transsphenoidal) repair of CSF leak with transcranial approach in terms of post-operative complications like infection, recurrence and hospital stay.
Material and Methods: The comparative experimental randomized study was conducted in the Department of Neurosurgery Unit I, PGMI, Lahore General Hospital, Lahore. After approval from ethical committee this study was carried out in our unit. 40 subjects with the history of CSF leak were randomly divided into two groups; one was treated with endonasal trans-sphenoidal repair and another was treated with trans-cranial approach, the subjects were followed up for 1 year. Detailed history, neurological examination, preoperative CT and MRI scans were performed in all patients.
Results: The mean age of patients with CSF leak was 25.58 ± 14.38 years. Among the patients, 17 were females and 23 were male. The mean age of the female was 31.70 ± 14.29 years. The mean age of the male was 21.04 ± 12.95. The recurrence was observed in 2 (10%) of the patients treated with endoscopic technique and 1 (5%) of the patients treated with trans-cranial approach. Those patients were successfully repaired in a second operation. Overall success rate was 92.5%. One patient (2.50%) among the trans-cranial approach develops infection which was treated successfully.
Conclusion: The endoscopic repair of CSF leak is both safe and effective and should be considered as the standard procedure of choice in most of the cases.
Anterior Cervical Corpectomy with Cage Fixation for Cervical Spondylotic Myelopathy
Authors: Anwar Ullah Muhammad , Usman Muhammad , Khan Faheem Ullah , . Jehanzeb , Jalal Abdul 
Published: January 2022

Objective: This study aimed to report the clinical outcome of anterior cervical corpectomy with cage fixation in patients with cervical spondylotic myelopathy.
Material and Methods: This observational retrospective study included 36 patients from the Neurosurgery department of Lady Ready Hospital MTI, Peshawar from 2014 January to 2015 December. After performing surgery, the patients were followed up for six months for neurological outcome and various post-operative complications such as infection, transient recurrent laryngeal palsy, screw displacement and improvements in paresthesias and gait ataxia.
Results: Most of the patients have no post-operative complications. Seventy-five percent (n = 27) of patients reported an immediate improvement in paresthesia and fine hand movements and gait. The major reported complications were implant failure (5.55%) and recurrent laryngeal nerve transient palsy in two patients (5.55%) each.
Conclusion: In patients with cervical spondylotic myelopathy, with anterior compression, cervical corpectomy with cage fixation is less invasive and an effective procedure with acceptable outcomes.