bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multivariable Integrated Evaluation of Hydrodynamic Modeling: A Comparison of Performance Considering Different Baseline Topography Data
Authors: Modi Prakat , Revel Menaka , Yamazaki Dai 
Published: August 2022

AbstractContinental‐scale river hydrodynamic modeling helps to understand the global hydrological cycle, and model evaluation is essential for calibration and performance assessment. Although many models have been evaluated using several variables separately, methods for the integrated multivariable assessment have yet to be established. We proposed an integrated multivariable evaluation method and an overall basin skill score (OSK) metric, which integrates the sub‐basin skill score, calculated by combining multiple variables' normalized Nash‐Sutcliffe efficiency values. The sub‐basin approach and OSK assess the model performance at the sub‐basin and basin scales and overcome the observation‐related limitations of different spatial dimensions. Evaluation of the CaMa‐Flood global river model for the Amazon Basin considering three variables (discharge, water surface elevation, and flooded area) and two baseline topography data (Shuttle Radar Topography Mission (SRTM) and Multi‐Error‐Removed Improved‐Terrain (MERIT) digital elevation models (DEMs)), shows that the proposed methodology can overcome the limitation of single‐variable evaluation in identifying multiple best parameter sets due to low sensitivity. CaMa‐Flood considering MERIT DEM achieves a single optimal parameter set with a maximum OSK of 0.57 compared with 0.52 for the SRTM DEM, and it shows the importance of accuracy of topography data for improvement in the performance of river hydrodynamic modeling. The proposed multivariable integrated evaluation with better topography data proved to help reduce equifinality and estimate the best parameter by maintaining the physical relationships among variables.
Energetic Proton Distributions in the Inner and Middle Magnetosphere of Jupiter Using Juno Observations
Authors: Shen Xiao‐Chen , Li Wen , Ma Qianli , Nishimura Yukitoshi , Daly Alec , Kollmann Peter , Mauk Barry , Clark George , Bolton Scott 
Published: August 2022

AbstractJupiter is known to have a complex magnetosphere containing energetic (above 10s of keV) electrons, protons, and heavy ions. However, a global distribution of these energetic particles is not fully understood before the era of the polar‐orbiting Juno mission. In this study, we focus on the energetic proton distribution at M < 50 by taking advantage of Juno's measurement covering various magnetic latitudes and M‐shells, and find that energetic proton fluxes are higher off‐equator than that near the equator at M > ∼20, and become comparable or lower at high latitudes than those near the equator at low M‐shells. Pitch angle distributions of energetic protons are field‐aligned, isotropic, and weak pancake‐like from high to low M‐shells. Proton phase space density shows a weak M‐shell dependence at M > 30 and a large positive slope at M < 30, suggesting their potential source and loss processes.
Integrated Modeling of Dynamic Marsh Feedbacks and Evolution Under Sea‐Level Rise in a Mesotidal Estuary (Plum Island, MA, USA)
Authors: Alizad Karim , Morris James T. , Bilskie Matthew V. , Passeri Davina L. , Hagen Scott C. 
Published: August 2022

AbstractAround the world, wetland vulnerability to sea‐level rise (SLR) depends on different factors including tidal regimes, topography, creeks and estuary geometry, sediment availability, vegetation type, etc. The Plum Island estuary (PIE) is a mesotidal wetland system on the east coast of the United States. This research applied a newly updated Hydro‐MEM (integrated hydrodynamic‐marsh) model to assess the impacts of intermediate‐low (50 cm), intermediate (1 m), and intermediate‐high (1.5 m) SLR on marsh evolution by the year 2100. Model advancements include capturing vegetation change, inorganic and below and aboveground organic matter portion of marsh platform accretion, and mudflat creation. Although the results indicate a low vulnerability marsh at the PIE, the vegetation changes from high to low marsh under all SLR scenarios (2%–22%), with the higher bounds belonging to higher rise scenarios. Lower SLR produces more productive marsh (13% gain in high productivity regions), whereas the highest SLR scenario causes increased tidal inundation, which leads to loss in productivity (12% change from high to low productivity regions), generation of mudflats (17% of the domain land), and marsh migration to higher lands. Sensitive nonlinear tidal flow changes, which may be increased or decreased with SLR as a result of mudflat creation, marsh migration, and bottom friction change, emphasize the importance of integrated modeling approaches that include dynamic marsh feedbacks in hydrodynamic modeling and varying hydrodynamic effects on the marsh system.
Gray and white matter abnormality in patients with T2DM-related cognitive dysfunction: a systemic review and meta-analysis
Authors: Ma Teng , Li Ze-Yang , Yu Ying , Hu Bo , Han Yu , Ni Min-Hua , Huang Yu-Xiang , Chen Hao-Han , Wang Wen , Yan Lin-Feng , Cui Guang-Bin 
Published: August 2022

Abstract
Aims/hypothesis
Brain structure abnormality in patients with type 2 diabetes mellitus (T2DM)-related cognitive dysfunction (T2DM-CD) has been reported for decades in magnetic resonance imaging (MRI) studies. However, the reliable results were still unclear. This study aimed to make a systemic review and meta-analysis to find the significant and consistent gray matter (GM) and white matter (WM) alterations in patients with T2DM-CD by comparing with the healthy controls (HCs).

Methods
Published studies were systemically searched from PubMed, MEDLINE, Cochrane Library and Web of Science databases updated to November 14, 2021. Studies reporting abnormal GM or WM between patients with T2DM-CD and HCs were selected, and their significant peak coordinates (x, y, z) and effect sizes (z-score or t-value) were extracted to perform a voxel-based meta-analysis by anisotropic effect size-signed differential mapping (AES-SDM) 5.15 software.

Results
Total 15 studies and 16 datasets (1550 participants) from 7531 results were involved in this study. Compared to HCs, patients with T2DM-CD showed significant and consistent decreased GM in right superior frontal gyrus, medial orbital (PFCventmed. R, BA 11), left superior temporal gyrus (STG. L, BA 48), and right calcarine fissure / surrounding cortex (CAL. R, BA 17), as well as decreased fractional anisotropy (FA) in right inferior network, inferior fronto-occipital fasciculus (IFOF. R), right inferior network, longitudinal fasciculus (ILF. R), and undefined area (32, −60, −42) of cerebellum. Meta-regression showed the positive relationship between decreased GM in PFCventmed.R and MoCA score, the positive relationship between decreased GM in STG.L and BMI, as well as the positive relationship between the decreased FA in IFOF.R and age or BMI.

Conclusions/interpretation
T2DM impairs the cognitive function by affecting the specific brain structures. GM atrophy in PFCventmed. R (BA 11), STG. L (BA 48), and CAL. R (BA 17), as well as WM injury in IFOF. R, ILF. R, and undefined area (32, −60, −42) of cerebellum. And those brain regions may be valuable targets for future researches. Age, BMI, and MoCA score have a potential influence on the altered GM or WM in T2DM-CD.
Exploratory analysis of eating- and physical activity-related outcomes from a randomized controlled trial for weight loss maintenance with exercise and liraglutide single or combination treatment
Authors: Jensen Simon Birk Kjær , Janus Charlotte , Lundgren Julie Rehné , Juhl Christian Rimer , Sandsdal Rasmus Michael , Olsen Lisa Møller , Andresen Anne , Borg Signe Amalie , Jacobsen Ida Christine , Finlayson Graham , Stallknecht Bente Merete , Holst Jens Juul , Madsbad Sten , Torekov Signe Sørensen 
Published: August 2022

AbstractWeight regain after weight loss remains a major challenge in obesity treatment and may involve alteration of eating and sedentary behavior after weight loss. In this randomized, controlled, double-blind trial, adults with obesity were randomized, in a 1:1:1:1 ratio stratified by sex and age group (<40 years and ≥40 years), to one-year weight loss maintenance with exercise, the GLP-1 receptor agonist liraglutide, or the combination, as compared with placebo, after low-calorie diet-induced weight loss. Primary outcome was change in body weight, which has been published. Here, we investigated the effects of weight loss maintenance with exercise, liraglutide, or the combination on weight loss-induced changes in the pre-specified explorative outcomes, eating and sedentary behavior in 130 participants who completed the trial according to the study protocol (exercise (n = 26), liraglutide (n = 36), combination (n = 29), and placebo (n = 39)). One year after weight loss, the placebo group had decreased postprandial appetite suppression score by 14%, and increased sedentary time by 31 min/day and regained weight. Liraglutide prevented the decrease in postprandial appetite suppression score compared with placebo (0% vs. −14%; P = 0.023) and maintained weight loss. Exercise after weight loss did not increase appetite or sedentary behavior compared with placebo, despite increased exercise energy expenditure and maintained weight loss. The combination of exercise and liraglutide increased cognitive restraint score (13% vs. −9%; P = 0.042), reflecting a conscious restriction of food intake, and decreased sedentary time by 41 min/day (−10 vs. 31 min/day; 95%CI, −82.3 to −0.2; P = 0.049) compared with placebo, which may have facilitated the additional weight loss. Targeting both eating and sedentary behavior could be the most effective for preventing weight regain.Trial registration: EudraCT number, 2015-005585-32; clinicaltrials.gov number, NCT04122716.
Circular EZH2-encoded EZH2-92aa mediates immune evasion in glioblastoma via inhibition of surface NKG2D ligands
Authors: Zhong Jian , Yang Xuesong , Chen Junju , He Kejun , Gao Xinya , Wu Xujia , Zhang Maolei , Zhou Huangkai , Xiao Feizhe , An Lele , Wang Xiuxing , Shi Yu , Zhang Nu 
Published: August 2022

AbstractGlioblastoma (GBM) is a highly aggressive primary brain tumour and is resistant to nearly all available treatments, including natural killer (NK) cell immunotherapy. However, the factors mediating NK cell evasion in GBM remain largely unclear. Here, we report that EZH2-92aa, a protein encoded by circular EZH2, is overexpressed in GBM and induces the immune evasion of GBM stem cells (GSCs) from NK cells. Positively regulated by DEAD-box helicase 3 (DDX3), EZH2-92aa directly binds the major histocompatibility complex class I polypeptide-related sequence A/B (MICA/B) promoters and represses their transcription; it also indirectly represses UL16-binding protein (ULBP) transcription by stabilizing EZH2. The downregulation of NK group 2D ligands (NKG2DLs, including MICA/B and ULBPs) in GSCs mediates NK cell resistance. Moreover, stable EZH2-92aa knockdown enhances NK cell-mediated GSC eradication in vitro and in vivo and synergizes with anti-PD1 therapy. Our results highlight the immunosuppressive function of EZH2-92aa in inhibiting the NK cell response in GBM and the clinical potential of targeting EZH2-92aa for NK-cell-directed immune therapy.
Structural basis of transcriptional regulation by a nascent RNA element, HK022 putRNA
Authors: Hwang Seungha , Olinares Paul Dominic B. , Lee Jimin , Kim Jinwoo , Chait Brian T. , King Rodney A. , Kang Jin Young 
Published: August 2022

AbstractTranscription, in which RNA polymerases (RNAPs) produce RNA from DNA, is the first step of gene expression. As such, it is highly regulated either by trans-elements like protein factors and/or by cis-elements like specific sequences on the DNA. Lambdoid phage HK022 contains a cis-element, put, which suppresses pausing and termination during transcription of the early phage genes. The putRNA transcript solely performs the anti-pausing/termination activities by interacting directly with the E.coli RNAP elongation complex (EC) by an unknown structural mechanism. In this study, we reconstituted putRNA-associated ECs and determined the structures using cryo-electron microscopy. The determined structures of putRNA-associated EC, putRNA-absent EC, and σ70-bound EC suggest that the putRNA interaction with the EC counteracts swiveling, a conformational change previously identified to promote pausing and σ70 might modulate putRNA folding via σ70-dependent pausing during elongation.
Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses
Authors: Schulz Rouven , Korkut-Demirbaş Medina , Venturino Alessandro , Colombo Gloria , Siegert Sandra 
Published: August 2022

AbstractG protein-coupled receptors (GPCRs) regulate processes ranging from immune responses to neuronal signaling. However, ligands for many GPCRs remain unknown, suffer from off-target effects or have poor bioavailability. Additionally, dissecting cell type-specific responses is challenging when the same GPCR is expressed on different cells within a tissue. Here, we overcome these limitations by engineering DREADD-based GPCR chimeras that bind clozapine-N-oxide and mimic a GPCR-of-interest. We show that chimeric DREADD-β2AR triggers responses comparable to β2AR on second messenger and kinase activity, post-translational modifications, and protein-protein interactions. Moreover, we successfully recapitulate β2AR-mediated filopodia formation in microglia, an immune cell capable of driving central nervous system inflammation. When dissecting microglial inflammation, we included two additional DREADD-based chimeras mimicking microglia-enriched GPR65 and GPR109A. DREADD-β2AR and DREADD-GPR65 modulate the inflammatory response with high similarity to endogenous β2AR, while DREADD-GPR109A shows no impact. Our DREADD-based approach allows investigation of cell type-dependent pathways without known endogenous ligands.
Sirt2 promotes white matter oligodendrogenesis during development and in models of neonatal hypoxia
Authors: Jablonska Beata , Adams Katrina L. , Kratimenos Panagiotis , Li Zhen , Strickland Emma , Haydar Tarik F. , Kusch Katharina , Nave Klaus-Armin , Gallo Vittorio 
Published: August 2022

AbstractDelayed oligodendrocyte (OL) maturation caused by hypoxia (Hx)-induced neonatal brain injury results in hypomyelination and leads to neurological disabilities. Previously, we characterized Sirt1 as a crucial regulator of OL progenitor cell (OPC) proliferation in response to Hx. We now identify Sirt2 as a critical promoter of OL differentiation during both normal white matter development and in a mouse model of Hx. Importantly, we find that Hx reduces Sirt2 expression in mature OLs and that Sirt2 overexpression in OPCs restores mature OL populations. Reduced numbers of Sirt2+ OLs were also observed in the white matter of preterm human infants. We show that Sirt2 interacts with p27Kip1/FoxO1, p21Cip1/Cdk4, and Cdk5 pathways, and that these interactions are altered by Hx. Furthermore, Hx induces nuclear translocation of Sirt2 in OPCs where it binds several genomic targets. Overall, these results indicate that a balance of Sirt1 and Sirt2 activity is required for developmental oligodendrogenesis, and that these proteins represent potential targets for promoting repair following white matter injury.
Effects of different concentrations of intraluminal sodium chloride solution on intracavitary ECG used for arm infusion port implantation
Authors: Dong Lei , Guan Chen-yang , Zhang Ying , Wang Ai-xia , Liu Ming-hua , Guo Chen , Hao Xiao-li , Zhang Qi 
Published: August 2022

AbstractAt present, there are few clinical studies on the application of high-concentration sodium chloride solutions in intracavitary ECG-guided catheter tip localization during the arm infusion port implantation. This study observed the effects of sodium chloride solutions with different concentrations on intracavitary ECG-guided arm infusion port implantation in the patients with cancer. The 657 patients receiving arm infusion port implantation in our hospital between January 2020 and August 2021 were randomly divided into 0.9% sodium chloride solution conduction group (group A), 5.45% sodium chloride solution conduction group (group B) and 10% sodium chloride solution conduction group (group C). The derived rate of stable intracavitary ECG, the occurrence rate of characteristic P wave, the time used for catheter tip localization and the optimal position rate of catheter tip were compared between the three groups. The derived rate of stable intracavitary ECG was significantly higher in the group B (97.78%) and group C (98.63%) than in the group A (93.90%) (all P < 0.05). The occurrence rate of characteristic P wave was also significantly higher in the group B (96.89%) and group C (97.72%) than in the group A (88.73%) (all P < 0.001). The time used for catheter tip localization was significantly shorter in the group B [(49.73 ± 8.15) s] and group C [(48.27 ± 8.61) s] than in the group A [(69.37 ± 19.99) s] (all P < 0.001). There was no significant difference in the optimal position rate of catheter tip among the three groups (P > 0.05). The 5.45% and 10% sodium chloride solutions are significantly superior comparing with 0.9% sodium chloride solution in the derived rate of stable intracavitary ECG, occurrence rate of characteristic P wave and time used for catheter tip localization, but there were no significant differences between 5.45 and 10% sodium chloride solutions. Moreover, the 5.45% sodium chloride solution is closer to physiological state comparing with 10% sodium chloride solution, so the 5.45% sodium chloride solution may be recommended for the intracavitary ECG-guided arm infusion port implantation.
Characterization of presence and activity of microRNAs in the rumen of cattle hints at possible host-microbiota cross-talk mechanism
Authors: Ricci Sara , Petri Renée M. , Pacífico Cátia , Castillo-Lopez Ezequias , Rivera-Chacon Raul , Sener-Aydemir Arife , Reisinger Nicole , Zebeli Qendrim , Kreuzer-Redmer Susanne 
Published: August 2022

AbstractMicroRNAs (miRNAs), as important post-transcriptional regulators, are ubiquitous in various tissues. The aim of this exploratory study was to determine the presence of miRNAs in rumen fluid, and to investigate the possibility of miRNA-mediated cross-talk within the ruminal ecosystem. Rumen fluid samples from four cannulated Holstein cows were collected during two feeding regimes (forage and high-grain diet) and DNA and RNA were extracted for amplicon and small RNA sequencing. Epithelial biopsies were simultaneously collected to investigate the co-expression of miRNAs in papillae and rumen fluid. We identified 377 miRNAs in rumen fluid and 638 in rumen papillae, of which 373 were shared. Analysis of microbiota revealed 20 genera to be differentially abundant between the two feeding regimes, whereas no difference in miRNAs expression was detected. Correlations with at least one genus were found for 170 miRNAs, of which, 39 were highly significant (r > |0.7| and P < 0.01). Both hierarchical clustering of the correlation matrix and WGCNA analysis identified two main miRNA groups. Putative target and functional prediction analysis for the two groups revealed shared pathways with the predicted metabolic activities of the microbiota. Hence, our study supports the hypothesis of a cross-talk within the rumen at least partly mediated by miRNAs.
Changes in lipids metabolism indices as a result of different form of selenium supplementation in chickens
Authors: Bień Damian , Michalczuk Monika , Szkopek Dominika , Kinsner Misza , Konieczka Paweł 
Published: August 2022

AbstractSelenium is an essential element that is important for many metabolic processes. Feed components used in chicken nutrition, especially cereals, may be deficient in selenium, hence selenium supplementation is necessary. Taking into account the progress in breeding, and thus the higher demand of birds for this element, it seems obvious to investigate an increased selenium dose in the diet of chickens. The aim of the study was to evaluate the effect of feed enriched with different forms of selenium at an increased dose of 0.5 mg/kg feed on the profile and metabolism of fatty acids in the breast muscle and liver of chickens. The study was conducted on 300 Ross 308 chickens reared for 42 days under standard conditions. The control group received feed supplemented with sodium selenite at a dose of 0.3 mg/kg feed. The research groups received different forms of selenium (sodium selenate, selenised yeast, nano-selenium) at an increased dose of 0.5 mg/kg feed. The study showed that the administration of different forms of selenium in the feed affected its concentration in the breast muscle and liver (p ≤ 0.01). Nano-selenium was found to have a high bioavailability, but also a lower risk of toxicity compared to other forms of selenium. Using different forms of selenium (p ≤ 0.01) at a dose of 0.5 mg/kg feed can significantly modify the fatty acid profile, lipid and enzymatic indices of fatty acid metabolism in breast muscle and liver.
Non-invasive stimulation with temporal interference: optimization of the electric field deep in the brain with the use of a genetic algorithm
Authors: Stoupis D , Samaras T 
Published: August 2022

Abstract

Objective. Since the introduction of transcranial temporal interference stimulation, there has been an ever-growing interest in this novel method, as it theoretically allows non-invasive stimulation of deep brain target regions. To date, attempts have been made to optimize the electrode montages and injected current to achieve personalized area targeting using two electrode pairs. Most of these methods use exhaustive search to find the best match, but faster and, at the same time, reliable solutions are required. In this study, the electrode combinations as well as the injected current for a two-electrode pair stimulation were optimized using a genetic algorithm, considering the right hippocampus as the region of interest (ROI). Approach. Simulations were performed on head models from the Population Head Model repository. First, each model was fitted with an electrode array based on the 10–10 international EEG electrode placement system. Following electrode placement, the models were meshed and solved for all single-pair electrode combinations, using an electrode on the left mastoid as a reference (ground). At the optimization stage, different electrode pairs and injection currents were tested using a genetic algorithm to obtain the optimal combination for each model, by setting three different maximum electric field thresholds (0.2, 0.5, and 0.8 V m−1) in the ROI. The combinations below the set threshold were given a high penalty. Main results. Greater focality was achieved with our optimization, specifically in the ROI, with a significant decrease in the surrounding electric field intensity. In the non-optimized case, the mean brain volumes stimulated above 0.2 V m−1 were 99.9% in the ROI, and 76.4% in the rest of the gray matter. In contrast, the stimulated mean volumes were 91.4% and 29.6%, respectively, for the best optimization case with a threshold of 0.8 V m−1. Additionally, the maximum electric field intensity inside the ROI was consistently higher than that outside of the ROI for all optimized cases. Objective. Given that the accomplishment of a globally optimal solution requires a brute-force approach, the use of a genetic algorithm can significantly decrease the optimization time, while achieving personalized deep brain stimulation. The results of this work can be used to facilitate further studies that are more clinically oriented; thus, enabling faster and at the same time accurate treatment planning for the stimulation sessions.