bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silenced LINC01134 Enhances Oxaliplatin Sensitivity by Facilitating Ferroptosis Through GPX4 in Hepatocarcinoma
Authors: Kang Xiaofeng , Huo Yan , Jia Songhao , He Fuliang , Li Huizi , Zhou Qing , Chang Nijia , Liu Donghui , Li Rongkuan , Hu Yi , Zhang Ping , Xu An 
Published: July 2022

PurposeRecently, long noncoding RNA LINC01134 has been shown to reduce cell viability and apoptosis via the antioxidant stress pathway, thereby enhancing OXA resistance in hepatocellular carcinoma. However, the association of LINC01134 with ferroptosis and the underlying molecular mechanisms remain to be elucidated.MethodsBioinformatics analysis was employed to screen lncRNAs positively correlated with GPX4 and poor clinical prognosis. And Western blot and RT-PCR analysis in HCC cells confirmed the effect of LINC01134 on GPX4 expression. In addition, LINC01134 siRNA was transfected in HCC cells to detect the changes in cell viability, ROS, lipid peroxidation, MDA levels and GSH/GSSG levels. CCK-8, colony formation and apoptosis assays were performed to determine the effect of LINC01134 on cell death. The effect of LINC01134 and OXA on Nrf2 transcriptional binding to GPX4 was analyzed using dual luciferase reporter assay and CHIP. The expression of GPX4 and Nrf2 in HCC tissues was detected by FISH and IHC.ResultsLINC01134 is a novel lncRNA positively correlated with GPx4 and associated with poor clinical prognosis. Silenced LINC01134 conferred OXA sensitivity by enhancing total ROS, lipid ROS, MDA levels and decreasing GSH/GSSG ratio. Mechanistically, LINC01134 and OXA could promote Nrf2 recruitment to the GPX4 promoter region to exert transcriptional regulation of GPX4. Clinically, LINC01134 was positively correlated with GPX4 or Nrf2, demonstrating the clinical significance of LINC01134, Nrf2 and GPX4 in OXA resistance of HCC.ConclusionsWe identified LINC01134/Nrf2/GPX4 as a novel and critical axis to regulate HCC growth and progression. Targeting GPX4, knocking down LINC01134 or Nrf2 could be a potential therapeutic strategy for HCC.
Chronic Lung Disease Following Neonatal Extracorporeal Membrane Oxygenation: A Single-Center Experience
Authors: Perez Ortiz Alba , Glauner Anna , Dittgen Felix , Doniga Thalia , Hetjens Svetlana , Schaible Thomas , Rafat Neysan 
Published: July 2022

ObjectiveTo assess the incidence and severity of chronic lung disease (CLD) after neonatal extracorporeal membrane oxygenation (ECMO) and to identify factors associated with its development.MethodsA retrospective observational study in a neonatal ECMO center was conducted. All neonates who received support with ECMO in our institution between January 2019 and October 2021 were included and their pulmonary outcome was investigated.ResultsA total of 91 patients [60 with congenital diaphragmatic hernia (CDH), 26 with meconium aspiration syndrome, and 5 with other diagnoses] were included in this study. Sixty-eight (75%) neonates survived. Fifty-two (76%) ECMO survivors developed CLD. There was no statistical difference between patients with and without CLD with regard to gender or gestational age. Patients with CLD had lower birth weight, were younger at the initiation of ECMO, and required longer ECMO runs. Patients with CDH developed CLD more often than infants with other underlying diseases (94 vs. 60%). Seventeen ECMO survivors (25%) developed severe CLD.ConclusionThe incidence of CLD after neonatal ECMO is substantial. Risk factors for its development include CDH as an underlying condition, the necessity for early initiation of ECMO, and the need for ECMO over 7 days.
Factors Surrounding the Healthcare Transition From Pediatric to Adult Care in 5p- Syndrome: A Survey Among Healthcare Professionals
Authors: Ishizaki Yuko , Matsuo Mari , Saito Kayoko , Fujihira Yoko 
Published: July 2022

BackgroundThe 5p- syndrome is associated with intellectual disturbance and physical complications from infancy, and patients continue treatment into adulthood. This study aimed to clarify the factors that facilitate and prevent healthcare transition from pediatric to adult care by conducting a questionnaire survey among medical professionals.SubjectsThe survey included 81 medical professionals nominated by an association of families of 5p- patients in Japan. The questions involved medical care for 5p- syndrome in adulthood, experience of transition, and factors facilitating a patient’s transition. Responses were obtained from 32 participants, with 27 answers eligible for analysis.ResultsThe questionnaire items involved physical symptoms and concerns regarding support and welfare prompting consult. The most common physical symptom was constipation. Regarding support and welfare, all participants had an experience of receiving consultation about care for the siblings of patients. Three (11.1%) participants had an experience of transition. Regarding the transition of patients with rare diseases or intellectual disturbance, only four (14.8%) believed that progress was being made in the transition.DiscussionOnly 11% of the respondents experienced the transition of patients with 5p- syndrome. Because it is difficult for highly specialized adult care providers to deal with multidisciplinary complications of 5p- syndrome and information on prognosis and natural history is not known, it is presumed that the transition of 5p- syndrome did not progress. Factors to improve the transition of patients with 5p- syndrome and are likely to be effective for the transition of patients with other rare diseases or intellectual disabilities.
Catalpol as a Component of Rehmannia glutinosa Protects Spinal Cord Injury by Inhibiting Endoplasmic Reticulum Stress-Mediated Neuronal Apoptosis
Authors: Huang Zhiyang , Gong Jiahong , Lin Wen , Feng Zhiyi , Ma Yirou , Tu Yurong , Cai Xiong , Liu Jianhua , Lv Chang , Lv Xinru , Wu Qiuji , Lu Wenjie , Zhao Juan , Ying Yibo , Li Shengcun , Ni Wenfei , Chen Haili 
Published: July 2022

Disturbance of the internal environment in the spinal cord after spinal cord injury (SCI) is an important cause of the massive death of neurons in the injury area and one of the major problems that lead to the difficult recovery of motor function in patients. Rehmannia glutinosa, a famous traditional Chinese medicine, is commonly used in neurodegenerative diseases, whereas an iridoid glycoside extract of catalpol (CAT), with antioxidant, antiapoptotic, and neuroprotective pharmacological effects. However, the neuroprotective and anti-apoptosis mechanism of CAT in SCI remains unclear. In our study, we found that CAT has a restorative effect on the lower limb motor function of rats with SCI by establishing a rat model of SCI and treating CAT gavage for 30 days. Our study further found that CAT has the effect of inhibiting apoptosis and protecting neurons, and the action pathway may reduce endoplasmic reticulum (ER) stress by inhibiting CHOP and GRP78 expression and then reduce apoptosis and protect neurons through the Caspase3/Bax/Bcl-2 pathway. In conclusion, we demonstrated that CAT can treat SCI by inhibiting ER stress-mediated neuronal apoptosis and has the potential to be a clinical drug for the treatment of SCI.
Exploring the Role of Social Connection in Interventions With Military Veterans Diagnosed With Post-traumatic Stress Disorder: Systematic Narrative Review
Authors: Gettings Richard D. , Kirtley Jenna , Wilson-Menzfeld Gemma , Oxburgh Gavin E. , Farrell Derek , Kiernan Matthew D. 
Published: July 2022

BackgroundIt has been identified that military veterans have distinct experiences of loneliness and social isolation and, when comparing this community to other client groups with a PTSD diagnosis, veterans respond less favorably to treatment. However, the link between PTSD and loneliness for veterans remains insufficiently researched and it is unclear if there are effective interventions tackling this distinct experience of loneliness.AimsThis systematic narrative review aimed to synthesize existing evidence incorporating elements of social connection, social isolation, and loneliness within interventions for military veterans with a diagnosis of PTSD, consequently aiming to examine the impact of such interventions upon this community.MethodsSix databases were searched, utilizing relevant search criteria, with no date restrictions. Articles were included if they involved intervention or treatment for military veterans with PTSD and considered elements of social connection, social isolation, and/or loneliness. The initial search returned 202 papers. After exclusions, removal of duplications, and a reference/citation search, 28 papers remained and were included in this review.ResultsFrom the 28 studies, 11 directly addressed social isolation and two studies directly addressed loneliness. Six themes were generated: (i) rethinking the diagnosis of PTSD, (ii) holistic interventions, (iii) peer support, (iv) social reintegration, (v) empowerment through purpose and community, and (vi) building trust.ConclusionsA direct focus upon social reintegration and engagement, psychosocial functioning, building trust, peer support, group cohesiveness and empowerment through a sense of purpose and learning new skills may mitigate experiential loneliness and social isolation for veterans with PTSD. Future research and practice should further explore the needs of the PTSD-diagnosed veteran community, seek to explore and identify potential common routes toward the development of PTSD within this community and consider bespoke interventions for tackling loneliness.
Job Demands, Resources and Burnout Among Polish Nurses During the Late Wave of COVID-19 Pandemic: The Mediating Role of Emotional Labor
Authors: Wójcik Grzegorz , Wontorczyk Antoni , Barańska Ilona 
Published: July 2022

ObjectiveBurnout has been recognized as a serious health problem. Nurses as a professional group are at a high risk of burnout occurrence, especially when facing burden associated with the COVID-19 pandemic. Despite evidence that higher job demands lead to burnout, there is less known about the indirect effect of job demands and resources on burnout via surface acting. Using the JD-R framework, this study examined how job demands and resources affected burnout among Polish nurses and whether these relationships are mediated by surface acting and moderated by coping with the workload.Materials and MethodsA sample of 270 nurses from the biggest hospital in Southern Poland filled out an online questionnaire at the time between the fourth and the fifth wave of the COVID-19 pandemic in Poland. The Polish adaptations of Oldenburg Burnout Inventory (OLBI), Organizational Constraints Scale (OCS), Interpersonal Conflict at Work Scale (ICAWS), Areas of Worklife Survey (AWS), and Emotional Labor Scale (ELS) were used. Mediation and moderation analyses were carried out in the SPSS macro-PROCESS.ResultsSurface acting partially mediated the positive association between organizational constraints and interpersonal conflict at work and burnout, as well as the negative association between the perceived organizational support and burnout. Coping with workload moderated the direct effect of organizational constraints on burnout via surface acting.ConclusionThe findings enrich the knowledge of the mediating and moderating mechanisms to explain the association between job demands, resources and burnout among nurses. There have been proposed interventions concerning increasing organizational support, effective emotional regulation of management education and psychological training regarding adequate coping strategies which could help reduce or prevent the occurrence of burnout in this professional group.
Facilitators and Barriers to Sustainable Employment After Spinal Cord Injury or Acquired Brain Injury: The Person's Perspective
Authors: Karcz Katarzyna , Schiffmann Barbara , Schwegler Urban , Staubli Stefan , Finger Monika E. 
Published: July 2022

BackgroundSustaining employment after initial return to work represents a major challenge for people with a disability. While individuals with spinal cord injury (SCI) and acquired brain injury (ABI) make a prime example for this challenge, their view on factors supporting and hindering sustainable employment have rarely been investigated in depth so far.PurposeTo examine facilitators and barriers to sustainable employment, as perceived by persons with SCI or ABI.MethodsFourteen focus groups and four individual interviews were conducted and thematically analyzed.ResultsPerceived facilitators and barriers to sustainable employment reflected the three biopsychosocial areas of personal, impairment-related and environmental factors. For both condition groups, key facilitators included environmental factors (i.e., aspects of the work organization, the workplace, supportive private and work environment) and personal factors (i.e., the ability to self-advocate, to communicate and to learn how to live with one's own disability). Major barriers comprised injury-related impairments, including decreased mobility and pain for people with SCI and fatigue and limited cognitive resources for persons with ABI, as well as environmental factors related to insurance procedures and the social security system for both conditions.ConclusionsThe biopsychosocial factors identified in our study as well as their interplay should receive particular attention to optimally support sustainable employment in vocational integration and work retention practice. Interventions should particularly focus on the empowerment of those affected as well as on the creation of supportive work environments that match their abilities and needs.
Analyzing Local Carbon Dioxide and Nitrogen Oxide Emissions From Space Using the Divergence Method: An Application to the Synthetic SMARTCARB Dataset
Authors: Hakkarainen Janne , Ialongo Iolanda , Koene Erik , Szeląg Monika E. , Tamminen Johanna , Kuhlmann Gerrit , Brunner Dominik 
Published: July 2022

Since the Paris Agreement was adopted in 2015, the role of space-based observations for monitoring anthropogenic greenhouse gas (GHG) emissions has increased. To meet the requirements for monitoring carbon dioxide (CO2) emissions, the European Copernicus programme is preparing a dedicated CO2 Monitoring (CO2M) satellite constellation that will provide CO2 and nitrogen dioxide (NO2) observations at 4 km2 resolution along a 250 km wide swath. In this paper, we adapt the recently developed divergence method to derive both CO2 and nitrogen oxide (NOx) emissions of cities and power plants from a CO2M satellite constellation by using synthetic observations from the COSMO-GHG model. Due to its long lifetime, the large CO2 atmospheric background needs to be removed to highlight the anthropogenic enhancements before calculating the divergence. Since the CO2 noise levels are large compared to the anthropogenic enhancements, we apply different denoising methods and compare the effect on the CO2 emission estimates. The annual NOx and CO2 emissions estimated from the divergence maps using the peak fitting approach are in agreement with the expected values, although with larger uncertainties for CO2. We also consider the possibility to use co-emitted NOx emission estimates for quantifying the CO2 emissions, by using source-specific NOx-to-CO2 emission ratios derived directly from satellite observations. In general, we find that the divergence method provides a promising tool for estimating CO2 emissions, alternative to typical methods based on inverse modeling or on the analysis of individual CO2 plumes.
Severe Blunt Liver Injury Complicated by Delayed Massive Hemobilia in a Toddler: A Case Report and Literature Review
Authors: Liu Xiaoming , Sun Qianqian , Sun Wenjing , Niu Qiong , Wang Zhu , Liu Chen , Fu Tingliang , Geng Lei , Li Xiaomei 
Published: July 2022

IntroductionUnintentional injuries remain a leading cause of disability among children. Although most of the pediatric patients suffering blunt liver injury can be successfully treated with non-operative therapy, the diagnosis and management of delayed life-threatening hemobilia following severe blunt liver injury, especially in the pediatric population, remain a challenge for clinicians.Case PresentationA previously healthy 2-year-old girl suffered a severe blunt liver injury related to an electric bike, which was inadvertently activated by herself. She initially received non-operative therapy and was in a stable condition in the first 2 weeks. On the 16th and 22nd postinjury days, the patient presented with life-threatening massive hemobilia, which was confirmed via repeat emergent gastroscopy and hepatic arterial angiography. An emergency selective transarterial embolization of the involved branch of the left hepatic artery was successfully performed. The patient recovered uneventfully, and long-term follow-up was needed owing to a mild dilatation of the left intrahepatic bile duct.DiscussionIncidental injury in children should be considered as a major public health issue and preventive measures should be taken to reduce its occurrence. Delayed massive hemobilia after severe blunt liver trauma is rare, and its accurate and timely diagnosis via emergency hepatic arterial angiography and selective angioembolization may allow prompt and optimal management to achieve good outcomes in the pediatric population.
PERENCANAAN KEBUTUHAN MATERIAL MENGGUNAKAN METODE LOT-SIZING DENGAN PENERAPAN METODE FORECASTING TIME SERIES DALAM PERENCANAAN PRODUKSI DI KONVEKSI XYZ
Authors: Anggela Pepy , Febriandini Febriandini , Wahyudi Tri 
Published: July 2022

Konveksi XYZ merupakan industri yang bergerak di bidang produksi sekaligus memasarkan atau melakukan penjualan baju gamis. Usaha ini didirikan pada tahun 2012 di Kabupaten Sambas, Kalimantan Barat. Berdasarkan data historis yang diperoleh, permintaan baju gamis di Konveksi XYZ mengalami fluktuasi tidak menentu yang menyebabkan perusahaan terkadang melakukan produksi dalam jumlah yang belebih dan memproduksi dalam jumlah yang tidak mencukupi, sehingga menyebabkan kegiatan penjualan yang tidak optimal. Tujuan dari penelitian ini adalah untuk mengoptimalkan rencana produksi untuk memenuhi permintaan di masa mendatang. Tahapan dalam penelitian ini adalah pengumpulan data, plotting data, peramalan dengan menggunakan metode time series, melakukan uji validasi terhadap metode yang terpilih, membuat RRP, melakukan perencanaan agregat, membuat jadwal induk produksi, membuat RCCP dan melakukan perencanaan kebutuhan material menggunakan 4 metode lot-sizing, yaitu metode LFL, EOQ, POQ dan FPR.. Hasil penelitian menunjukkan bahwa metode terbaik adalah metode centered moving average, dikarenakan memiliki nilai MSE terkecil yaitu 4050,13, MAD terkecil yaitu 47,11 serta MAPE terkecil yaitu 11,06%. Total biaya yang dibutuhkan untuk produksi baju gamis selama 12 periode yaitu sebesar Rp 319.120.000,00. Sedangkan perencanaan kebutuhan bahan baku digunakan metode LFL dan metode POQ, dengan total biaya bahan baku baju gamis yang diperlukan untuk 12 periode kedepan yaitu sebesar Rp 428.884.300. Diperoleh biaya untuk masing-masing bahan baku yaitu, bahan baku kain sebesar Rp 456.460.000, resleting sebesar Rp 10.448.000, kancing baju sebesar 1.036.000, kain vislin sebesar Rp 1.840.000, benang jahit sebesar Rp 7.836.000 dan benang obras sebesar Rp 5.224.000. . Sehingga dampak dari penelitian ini ialah, dapat meminimalkan biaya kebutuhan bahan baku.
ANALISIS PERBAIKAN KUALITAS PELAYANAN UNTUK MENINGKATKAN KEPUASAN PELANGGAN DENGAN MENGGUNAKAN METODE SERVQUAL, IPA, DAN REGRESI LINEAR BERGANDA
Authors: Saputra Dandi Dwi 
Published: July 2022

Berkembangnya sepeda motor dari tahun ke tahun makin meningkat, populasi kendaraan sepeda motor membutuhkan perawatan atau servis rutin agar motor tetap layak jalan. Muncul banyak bengkel-bengkel kompetitor membuat persaingan semakin meningkat dan memikirkan cara memberikan pelayanan yang sesuai harapan pelanggan. Terjadinya pandemi membuat banyak sektor usaha mengalami penurunan omset pendapatan.Bagaimana menganalisis tingkat kepuasan pelanggan dan faktor apa saja yang mempengaruhi kualitas pelayanan dengan tujuan memperbaiki kualitas pelayanan. Metode Service Quality adalah metode yang sering digunakan untuk mengukur kualitas layanan, Importance Performance Analysis mencari prioritas atribut pelayanan, dan regresi linear berganda mengetahui pengaruh kualitas pelayanan terhadapat kepuasan pelanggan.Dari hasil perhitungan service quality menunjukkan tingkat kinerja dimensi pada umumnya lebih tinggi dibandingkan dengan kinerja perusahaan sehingga menghasilkan nilai gap pada tiap dimensinya bernilai positif. Terdapat 1 atribut pertanyaan yang masuk kuadran ini dan menjadi prioritas perbaikan .berdasarkan model regresi ppengaruh dimensi kualitas pelayanan terhadap kepuasan pelanggan adalah sebesar 63.5%, sedangkan 36,5.% dipengaruhi oleh variabel lain di luar penelitian.maka dapat disimpulkan bahwa secara umum layanan yang diberikan oleh PT.Thamrin Brothers Sako Kenten sudah memenuhi harapan para pelanggan, dan penyebab naik turunnya omset diperkiraan ada faktor dari luar.s, didapatkan atribut yang masuk dalam kuadran 1 dan menjadi prioritas utama dalam perbaikan, yaitu karyawan menginformasikan kerusakan kendaraan, disimpulkan keempat dimensi kualitas pelayanan tidak berpengaruh terhadap kepuasan pelanggan. Sedangkan satu atribut pertanyaan berpengaruh terhadap kepuasan pelanggan.
Kata Kunci: Kualitas Layanan, Service Quality, IPA, Regresi linear berganda
Pengembangan Media Pembelajaran E-Modul Menggunakan Sigil pada Kelas X RPL
Authors: Hariyandi Andriyas , Indah Juwita Astri , Maviza Sari Tessya 
Published: July 2022

This study aims to determine the Effectiveness of E-Module Learning Media Using Sigil in Vocational Basics Subjects at SMKN 2 Padang. This research uses research & development method. The research subjects were 35 students. The research model used is the development research model (ADDIE) with the development steps as follows. (1) Analyze, (2) Design, (3) Development, (4) Implementation and (5) Evaluate. By obtaining the results of the validity test by the experts, the overall assessment of the validator test on the E-Module using Sigil in the Class X RPL Vocational Basics Subject at SMKN 2 Padang Odd Semester is 81.19%, it can be interpreted that Valid is used. The results of the practical test of 90.57%, can be interpreted as very practical to use. The results of the research on the effectiveness test of 87.31% can be interpreted as very effective in use. In conclusion, based on the assessment and input of experts as well as the results of the E-Modul field trial using Sigil as a learning medium, it has been tested for feasibility, excellence, and can be used in the learning process in the subjects of Vocational Basics class X RPL at SMKN 2 Padang.