bluuebunny/crossref_metadata_2025_split huggingface dataset:
واقع القيادة التكنولوجية لمدراء مديريات الشباب و الرياضة في المنطقة الجنوبية من وجهة نظر العاملين
Authors: سمر هادي  , بشرى كاظم  
Published: June 2022

تكمن اهمية البحث في تناول بالوصف و التحليل مفهوم جديد للقيادة وهو القيادة التكنولوجية اذ ان القيادة التكنولوجية تحتاج الى العديد من اساليب علمية دقيقة و تقنيات تتطلب خبرات و تخصصات رائدة مما يتطلب من القائمين على اداء عملهم بصوره عالية من خلال تحقيق القيادة التكنلوجية ليتم انجازها لذلك ينبغي لها يتيح في الكليات التربية البدنية و علوم الرياضة وأن يوفران للقائمين عليها الامكانيات المادية و المالية اللازمة للوصول الى تطبيق القيادة التكنلوجية .، اما مشكلة البحث من خلال اطلاع الباحثتان على بعض المراجع و المصادر العلمية التي كانت تهتم بالتطورات الحديثة من خلال استخدام التكنولوجيا في العديد من المهام و المجالات و رغم ان العصر الحالي يعاني من الكثير من المشاكل و من اهمها سبب انتشار فايروس كورونا اذ اهتمت المؤسسات التربوية و التعليمية في استخدم التكنولوجيا في العديد من مجالاتها , اما منهج البحث استعملت الباحثتان المنهج الوصفي بأسلوبي المسحي , حددت الباحثتان مجتمع البحث بالطريقة العمدية من العاملين في مديرية وزارة الشباب و الرياضة و البالغ عدده (1297), إما عينة البحث فقد مثلت مجتمع البحث بأكمله , بحيث كانت اهم الاستنتاجات بناء مقياس القيادة التكنولوجية لإعضاء الهيئة التدريسية في بعض كليات التربية البدنية و علوم الرياضة , اما كانت اهم التوصيات استعمال هذه المقياس بالمؤسسات التعليمية والرياضية كافة , اجراء دراسات مشابهة لمقياس (القيادة التكنولوجية )وبكافة محاورهما للاستفادة من هذه الدراسة .
اثر قوة الأنا وعلاقتها بمستوى الطموح الرياضي لدى لاعبي بعض أندية مدينة الموصل المتقدمين
Authors: احمد سعيد رشيد  
Published: June 2022

التعرف على درجة قوة الانا ودرجة الطموح الرياضي لدى لاعبي بعض اندية مدينة الموصل المتقدمين,والتعرف على العلاقة بين قوة الاناومستوى الطموح الرياضي لدى لاعبي بعض اندية مدينة الموصل المتقدمين,واستخدم الباحث المنهج الوصفي بأسلوب الدراسات الارتباطية لملاءمته وطبيعة البحث. ويتمثل مجتمع البحث من لاعبي منتخبات مدينة الموصل المتقدمين والبالغ عددهم (334) لاعبا واشتملت عينة الدراسة على لاعبي بعض اندية مدينة الموصل المتقدمين والبالغ عددهم (78) لاعبا وبنسبة (23,3532%) وتم اختيار العينة بالطريقة العمدية.واستخدم الباحث مقياس (قوة الانا) ومقياس (مستوى الطموح الرياضي) , واستخدم الباحث الوسائل الإحصائية والتي تم حسابه باستخدام برنامج الحاسوب الآلي ( SPSS ) .وتوصل الباحث إلى عدد من الاستنتاجات أهمها: يتسم لاعبي بعض اندية مدينة الموصل المتقدمين بمستوى مرتفع في قوة الأنا, ومستوى الطموح الرياضي,وتوجد علاقة ارتباطية ايجابية بين ذات دلالة احصائية بين قوة الأنا ومستوى الطموح لدى لاعبي بعض اندية مدينة الموصل المتقدمين,وأهم التوصيات والمقترحات التي توصل اليها الباحث هي, حث المدربين على الإهتمام بالإعداد النفسي للاعبين الى جانب الاعداد البدني والمهاري والخططي وخاصة فيما يتعلق بالعوامل الشخصية ومنه قوة الأنا, وحث المدربين والقائمين على النشاط الرياضي في مدينة الموصل بتنظيم محاضرات تثقيفية تدور حول مفهوم قوة الأنا ومستوى الطموح وذلك لزيادة الوعي الصحي النفسي بحيث يتعرف اللاعبون على السمات الايجابية في شخصياتهم وعلى السبل التي تساهم في تنميتها,واشراك اختصاصي نفسي ضمن الملاك التدريبي لاندية مدينة الموصل للمتقدمين
ĐÁNH GIÁ KẾT QUẢ SỚM ĐIỀU TRỊ ĐIỀU TRỊ NANG TUYẾN VÚ BẰNG VI SÓNG TẠI ĐƠN VỊ TUYẾN VÚ BỆNH VIỆN CHỢ RẪY
Authors: Huỳnh Quang Khánh , Nguyen Thành Phát 
Published: June 2022

Đặt vấn đề: Nang tuyến vú thường gặp ở phụ nữ tiền mãn kinh (65.1%) so với phụ nữ mãn kinh (39.4%) với độ tuổi thường gặp nhất là từ 35 đến 50 tuổi. Can thiệp điều trị nang tuyến vú có triệu chứng bằng vi sóng với nhiều ưu điểm là một phương pháp điều trị đáng quan tâm.
Mục tiêu: Đánh giá kết quả sớm của phương pháp điều trị nang lành tính tuyến vú bằng vi sóng dưới hướng dẫn của siêu âm.
Đối tượng – Phương pháp nghiên cứu: Mô tả loạt ca hồi cứu bệnh nhân có nang tuyến vú có triệu chứng không đáp ứng điều trị nội khoa và chọc hút bằng kim nhỏ được can thiệp điều trị bằng vi sóng tại đơn vị Tuyến Vú bệnh viện Chợ Rẫy từ tháng 08/2018 đến tháng 03 /2020.
Kết quả: 58 bệnh nhân được điều trị bằng vi sóng, tuổi trung bình 45.3 tuổi. Nang đơn giản, nang phức hợp, nang phức tạp chiếm tỷ lệ lần lượt 80 %, 16.93 % và 3.07 %. Kích thước trung bình nang được can thiệp là 23.86 mm. Tất cả các bệnh nhân có đáp ứng hoàn toàn sau khi thực hiện đốt vi sóng, tỷ lệ giảm kích thước sau 06 tháng đạt trên 90%. Hoàn toàn không xuất hiện các biến chứng liên quan tới can thiệp trong suốt quá trình theo dõi. Có 01 trường hợp nang bọc sữa có vôi hóa thành nang không đáp ứng điều trị.
Kết luận: Can thiệp bằng vi sóng là một phương pháp điều trị hứa hẹn cho các nang tuyến vú có triệu chứng với tỉ lệ thành công thủ thuật cao, tỉ lệ biến chứng sau thủ thuật thấp. Nang có thành nang vôi hóa cần được đánh giá kỹ trước khi thực hiện điều trị bằng vi sóng
ĐÁNH GIÁ HIỆU QUẢ TIÊM NGOÀI MÀNG CỨNG CỘT SỐNG THẮT LƯNG QUA LỖ LIÊN HỢP DƯỚI HƯỚNG DẪN CỦA CẮT LỚP VI TÍNH
Authors: Đinh Gia Khánh 
Published: June 2022

Mục tiêu: Đánh giá hiệu quả kỹ thuật tiêm ngoài màng cứng qua đường lỗ liên hợp dưới hướng dẫn của cắt lớp vi tính để điều trị đau cột sống thắt lưng
Đối tượng và phương pháp nghiên cứu: 80 bệnh nhân thoát vị đĩa đệm cột sống thắt lưng được chẩn đoán bằng MRI tại Trung tâm chẩn đoán hình ảnh, bệnh viện Trung ương Quân đội 108 từ tháng 6 năm 2020 đến tháng 6 năm 2021. Nghiên cứu hồi cứu kết hợp tiến cứu
Kết quả: Vị trí phong bế rễ L4 11,3%, L5 70 %, S1 18,7%. Điểm VAS trước can thiệp, sau can thiệp 1 ngày, 14 ngày, 1 tháng, 3 tháng lần lượt là 6,48 ± 1,46; 3,21 ± 1,53; 2,74 ± 1,37; 2,55 ± 1,44; 3,35 ± 1,39. Điểm ODI trước can thiệp, sau can thiệp 1 ngày, 14 ngày, 1 tháng, 3 tháng lần lượt là 59,26 ± 12,18; 35,68 ± 7,97; 33,63 ± 6,14; 30,18 ± 6,01; 31,15 ± 6,46. Khác biệt có ý nghĩa thống kê
Kết luận: Tiêm ngoài màng cứng qua lỗ liên hợp là phương pháp điều trị đau có hiệu quả và tăng khả năng hoạt động cho bệnh nhân thoát vị đĩa đệm cột sống thắt lưng
NGHIÊN CỨU ĐẶC ĐIỂM HÌNH ẢNH VÀ VAI TRÒ CỦA 18FDG-PET/CT TRONG CHẨN ĐOÁN GIAI ĐOẠN CỦA BỆNH NHÂN UNG THƯ PHỔI KHÔNG TẾ BÀO NHỎ.
Authors: Mai Huy Thông 
Published: June 2022

Mục tiêu: Nghiên cứu đặc điểm hình ảnh 18FDG-PET/CT của các tổn thương trong ung thư phổi không tế bào nhỏ trước điều trị và vai trò của 18FDG-PET/CT trong chẩn đoán giai đoạn của bệnh nhân ung thư phổi không tế bào nhỏ.Đối tượng và phương pháp: Nghiên cứu hồi cứu kết hợp tiến cứu, phân tích mô tả trên 43 bệnh nhân ung thư phổi không tế bào nhỏ được chụp 18FDG-PET/CT mới phát hiện tại Bệnh viện quân y 103 từ tháng 02/2017 – tháng 02/2022, trong đó có 32 bệnh nhân được chỉ định phẫu thuật sau chụp 18FDG-PET/CT mà chưa trải qua biện pháp điều trị đặc hiệu nào.Kết quả: Kích thước trung bình khối u phổi là 40,98±21,53mm, có sự liên quan giữa chỉ số SUVmax và kích thước khối u nguyên phát. Giá trị SUVmax tăng theo giai đoạn T. Giá trị SUVmax trung bình của nhóm hạch >10mm là 10,59±6,12 cao hơn của nhóm hạch <10mm là 5,56±2,47. Sau chụp PET/CT n giai đoạn N0 chiếm 32,6%, giai đoạn N1 chiếm 23,3%, giai đoạn N2 chiếm 23,3%, giai đoạn N3 chiếm 20,9%. Giá trị SUVmax trung bình khối u ở nhóm bệnh nhân giai đoạn M1 là 15,96±4,29 cao hơn ở nhóm bệnh nhân giai đoạn M0 là 14,57±8,26.18FDG-PET/CT phát hiện được 100 % tổn thương u nguyên phát tại phổi, 18FDG-PET/CT chẩn đoán chính xác giai đoạn T ở 71,88 %, cao hơn so với CLVT với 68,75 %, chẩn đoán chính xác giai đoạn N ở 81,25%, cao hơn so với CLVT với 75 %. Độ nhạy, độ đặc hiệu, giá trị dự đoán dương tính, giá trị dự đoán âm tính của 18FDG-PET/CT trong chẩn đoán giai đoạn N lần lượt là: 100%; 68,42%; 68,42% và 100%.
*Từ khóa: Ung thư phổi không tế bào nhỏ, 18FDG-PET/CT, SUVmax.
Features of the course of comorbid somatic pathology depending on the method of treatment of lower jaw fracture without displacement of fragments
Authors: Moskalev Eugeniu , Bayrikov Ivan M. , Gubarevа Irina V. , Samikin Aleksandr S. , Trusov Yuriy A. , Zinkina Anna A. , Meshcheryakov Yuri V. 
Published: June 2022

AIM: The aim is to study the effect of the method of reducing the fractures of the mandible without displacement of fragments on the course of comorbid somatic pathology

MATERIALS AND METHODS: The study included 60 patients (45 men and 15 women) aged 18 to 69 years with fractures of the mandible without displacement of fragments and comorbid somatic pathology. The subjects were divided into three groups. The first group included 20 patients who received intraosseous orthodontic screws with intermaxillary rubber traction. The second group consisted of 20 patients who underwent immobilization with a Tigerstedt splint with intermaxillary rubber traction. The third group included 20 patients who underwent immobilization with a Vasiliev double-mandibular splint with intermaxillary rubber traction. We studied the effect of the method of reducing of fractures of the mandible without displacement of fragments on the course of comorbid somatic pathology.
RESULTS: As a result of the treatment in the first group, angina pectoris became unstable in 1 patient, which required a consultation with a cardiologist and correction of treatment. In the second group, in 3 patients (15%) with chronic polyarthrosis, prolonged wearing of dental splints resulted in the aggravation of deforming osteoarthritis of the TMJ and longer outpatient rehabilitation was required. In the third group, 13 patients (65%) with diabetes mellitus and ischemic heart disease demanded a prolonged oral tissue regeneration.
CONCLUSIONS: When choosing a method for reducing a fracture of the lower jaw, it is necessary to take into account the features of comorbid somatic pathology. The conservative method of treatment using dosed intermaxillary elastic traction is the most gentle.
Pengetahuan Dan Sikap Tentang Hipertensi Dengan Pengendalian Tekanan Darah
Authors: Simanjuntak Edriyani Yonlafado , Situmorang Henny 
Published: June 2022

Tujuan: Mengidentifikasi pengetahuan dan sikap tentang hipertensi dengan pengendalian tekanan darah pada penderita hipertensi.
Metode: Penelitian merupakan deskriptif kolerasi dengan menggunakan pendekatan Cross-Sectional dengan populasi seluruh pasien hipertensi di wilayah kerja Puskesmas Kecamatan Medan Deli dengan teknik pengambilan sampel asidental sampling berjumlah 54 orang. Instrumen penelitian menggunakan kuesioner dan data dianalisis secara univariat dalam bentuk distribusi frekwensi dan persentase dan bivariat menggunakan uji Chi Square Test.
Hasil: Menunjukkan bahwa hubungan pengetahuan dengan pengendalian tekanan darah pada pasien hipertensi diperoleh hasil p value = 0,004 dan hubungan sikap dengan pengendalian tekanan darah pada pasien hipertensi diperoleh hasil p value = 0,002.
Kesimpulan: Ada hubungan pengetahuan dan sikap dengan pengendalian tekanan darah pada pasien hipertensi. Diharapkan penderita hipertensi dapat menambah pengetahuan dan mampu bersikap dalam mengendalikan tekanan darah sehingga penyakit hipertensi yang dialami dapat dikontrol ataupun diminimalisir.
Pemenuhan kebutuhan pengetahuan dasar penyakit stroke pada masyarakat awam
Authors: Zati Rahayu Gumelar , Maulidiyah Hera , Monika Hani , Fauziah Halidah , Erik Irawan Bangbang 
Published: June 2022

Introduction:Cerebrovascular injury or often referred to as a stroke is a sudden occurrence of neurological deficits due to the failure of the blood supply to a part of the brain. Insufficiency or interruption of the blood supply caused by thrombosis, usually secondary to arteriosclerosis, to embolism originating elsewhere in the body, or to bleeding from ruptured arteries.
Objective: The activities carried out are to provide fulfillment of basic knowledge needs about this stroke. Stroke, also known as screbrovascular injury (CVA) is the cessation of blood supply to the brain and causes the function of part of the brain to disappear.
Method: The method used in this counseling is based on strategy, implementation and action, ending with an evaluation carried out at the end of the extension with the number of questionnaires given to the community for the needs of the required research results.
Results: The fulfillment of the need for basic knowledge about stroke to the community and the community can implement the results of counseling in accordance with the directions that have been given.
Conclusion: The implementation is divided into several dimensions, namely timeliness and duration, participation, knowledge and problem solving. Work and evaluation carried out by 5 observators.
Peningkatan Pengetahuan tentang Bahaya HIV/AIDS dengan Permainan Kartu di Pondok Pesantren
Authors: Firmansyah  , Ayu Nur Azizah Riska , Kustika Ade , Ernawati  , Reza Husaeni Sahrul , Latifah Siti , Nurapandi Adi 
Published: June 2022

Introduction: HIV is a virus that attacks the immune system, the cause of the emergence of AIDS which will make it difficult for the human body to fight disease due to a weakened immune system. Every year new cases continue to be found and West Java occupies the fourth position with the most cases in Indonesia during 2020.
Objective: to increase the knowledge of students about the dangers of HIV/AIDS and invite them to jointly prevent it
Method: The activities carried out were pretest, educational card game, quiz discussion, and posttest. This game uses card media consisting of 36 cards for two groups, each group gets 18 cards. The cards consist of 6 quiz cards, 6 correct answer cards, and 6 wrong answer cards. The quiz on the card is a quiz about HIV/AIDS. Then all participants are invited one by one to come forward to take a quiz card and look for the right answer card within 10 seconds.
Result: The knowledge of the students of the Al-Hasan Islamic Boarding School has increased with the implementation of community service programs through the media of this card game. Where almost all correspondents can answer the quiz correctly after playing the game. They can find out more about what HIV/AIDS is, so that they can prevent the occurrence of HIV/AIDS in their surrounding environment.
Conclusion: This educational card game can increase the knowledge of students who are still lacking about HIV/AIDS. With a fun method they can better understand and know how to respond to HIV/AIDS.
Survey of Snakes Bites among Snake Endemic Communities in North Eastern Nigeria
Authors: Manjur Shah Mohammad , Sabiu Imam Tijjani , Bala Aisha , Tukur Zainab 
Published: June 2022

Snake envenomation is increasingly recognized as a serious, worldwide public health concern and a neglected tropical disease of global importance especially in the North Eastern Nigeria. The scarcity of data regarding such snake fauna couple with its ability to inflict immense misery to the poorest of the population justifies the need to identify such snakes and some of the clinical features of snakebite victims in these endemic areas. Both primary and secondary data were collected during the study. Result revealed that 10 venomous snake species were reported in Gombe, Taraba and Bauchi state. The most abundant snake species is the Echis ocellantus (Carpet or saw scaled viper) having the highest frequency of encounter followed by the Bitis arientans (Puff Adder) and Naja nigricolis (Black Spiting Cobra). The Kaltungo General Hospital in Gombe is one of the major treatment centers in the North-Eastern Nigeria. About 2945 Human snakebite cases were reported in the Hospital in the year 2018, the highest snake envenoming were observed in October with 16.1% frequency while January has the least snakebite cases of 1.7%. The burden of snakebite envenoming in the North-Eastern Nigeria is a serious public health challenge which desperately need to be addressed.
Emotional and Behavioral Performance among Adolescents Attending at Outpatient Departments of Bangabandhu Sheikh Mujib Medical University during COVID-19 Pandemic
Authors: Bristi Syfunnahar , Haseen Fariha , Hasan Md. , Khan Niaz Mohammad , Ahmed Helal Uddin , Islam Syed Shariful 
Published: June 2022

The purpose of the research was to assess emotional and behavioral presentation among adolescents during the COVID-19 pandemic visiting outpatient departments of Bangabandhu Sheikh Mujib Medical University (BSMMU) and to compare the extent of emotional and behavioral symptoms among adolescents with key factors related to COVID-19. A cross-sectional study was conducted among adolescents from 11–17 years of age attending six outpatient departments of Bangabandhu Sheikh Mujib Medical University (BSMMU) from October to December 2020. A purposive sampling technique was applied and total 146 samples were selected. A validated Bangla SDQ scale of youth self-report measure (11 to 17 years) baseline version was used for data collection. Data were collected by a face-to-face interview. The study protocol was approved by Institutional Review Board (IRB) of Bangabandhu Sheikh Mujib Medical University (BSMMU). Both descriptive and inferential statistics were executed for data analysis by using SPSS-25 and MS Excel Software. On basis of SDQ-25 scale, among the three variables, around one–third respondents (34%) had emotional symptoms while 31% and 17% had conduct symptoms and hyperactivity symptoms respectively during the COVID-19 pandemic. Among the adolescents who had a history of positive symptoms for COVID-19, 26.5% had hyperactivity symptoms, on the other hand adolescents who had no history of positive symptoms for COVID-19, only 9.8% had significant hyperactivity symptoms. There was a highly significant association (p=0.004) present between behavioral (hyperactivity) symptoms and having a history of positive symptoms for COVID-19. Adolescents who had wariness about uncertainty of COVID-19 pandemic, 25% had significant emotional symptoms on the other hand who had no wariness about uncertainty of COVID-19 pandemic, 14.3% had emotional symptoms. There was significant association (p=0.048) present between emotional symptoms with ‘felt wariness about uncertainty of COVID -19 pandemic. There was also an association (p=0.035) present between behavioral (hyperactivity) symptoms with ‘relationship status among friends. A higher proportion of female adolescents had emotional and hyperactivity symptoms than male adolescents. However, male adolescents suffered mostly for conduct symptom compared to female. Adolescents attending in selected outpatient departments of BSMMU, were especially vulnerable to emotional and behavioral problem based on symptoms developing during COVID-19 pandemic.
A Monolithic Silicon‐Mesoporous Carbon Photosupercapacitor with High Overall Photoconversion Efficiency
Authors: Berestok Taisiia , Diestel Christian , Ortlieb Niklas , Glunz Stefan W. , Fischer Anna 
Published: June 2022

AbstractOff‐grid devices require autonomous power supply systems that can be achieved via coupling a solar cell with a supercapacitor in one integrated multifunctional device that is able to harvest energy from the environment, store, and release it on demand. Herein, a straight‐forward approach is proposed to realize a monolithic photosupercapacitor rechargeable under illumination by integrating a silicon (Si) solar cell with an electrochemical double‐layer capacitor (EDLC) based on mesoporous N‐doped carbon nanospheres (MPNC) in a three‐electrode configuration, i.e., via a shared electrode. The optimized porous structure of the MPNC‐EDLC electrodes results in a high storage efficiency of 95% and a superior performance compared to an activated carbon based EDLC. When monolithically integrated with a highly compatible and technologically robust Si solar cell in a photosupercapacitor, fast charging up to 0.63 V (2.5 V for a module of four photosupercapacitors connected in series) in less than 5 s is attained even under weak illumination conditions, with an outstanding peak overall efficiency of 11.8%. These results show high potential toward the development of photorechargeable and decentralized power sources for deployed smart electronic devices.
Chitosan‐Gelatin‐EGCG Nanoparticle‐Meditated LncRNA TMEM44‐AS1 Silencing to Activate the P53 Signaling Pathway for the Synergistic Reversal of 5‐FU Resistance in Gastric Cancer
Authors: Zhou Mi , Dong Jiaqi , Huang Junqing , Ye Wen , Zheng Zhousan , Huang Kangbo , Pan Yihui , Cen Junjie , Liang Yanping , Shu Guannan , Ye Sheng , Lu Xuanxuan , Zhang Jiaxing 
Published: June 2022

AbstractChemoresistance is one of the leading causes of therapeutic failure in gastric cancer (GC) treatment. Recent studies have shown lncRNAs play pivotal roles in regulating GC chemoresistance. Nanocarriers delivery of small interfering RNAs (siRNAs) to silence cancer‐related genes has become a novel approach to cancer treatment research. However, finding target genes and developing nanosystems capable of selectively delivering siRNAs for disease treatment remains a challenge. In this study, a novel lncRNA TMEM44‐AS1 that is related to 5‐FU resistance is identified. TMEM44‐AS1 has the ability to bind to and sponge miR‐2355‐5p, resulting in the upregulated PPP1R13L expression and P53 pathway inhibition. Next, a new nanocarrier called chitosan‐gelatin‐EGCG (CGE) is developed, which has a higher gene silencing efficiency than lipo2000, to aid in the delivery of a si‐TMEM44‐AS1 can efficiently silence TMEM44‐AS1 expression to synergistically reverse 5‐FU resistance in GC, leading to a markedly enhanced 5‐FU therapeutic effect in a xenograft mouse model of GC. These findings indicate that TMEM44‐AS1 may estimate 5‐FU therapy outcome among GC cases, and that systemic si‐TMEM44‐AS1 delivery combined with 5‐FU therapy is significant in the treatment of patients with recurrent GC.
Hierarchical Accumulation of Histone Variant H2A.Z Regulates Transcriptional States and Histone Modifications in Early Mammalian Embryos
Authors: Liu Xin , Zhang Jingjing , Zhou Jilong , Bu Guowei , Zhu Wei , He Hainan , Sun Qiaoran , Yu Zhisheng , Xiong Wenjing , Wang Liyan , Wu Danya , Dou Chengli , Yu Longtao , Zhou Kai , Wang Shangke , Fan Zhengang , Wang Tingting , Hu Ruifeng , Hu Taotao , Zhang Xia , Miao Yi‐Liang 
Published: June 2022

AbstractEarly embryos undergo extensive epigenetic reprogramming to achieve gamete‐to‐embryo transition, which involves the loading and removal of histone variant H2A.Z on chromatin. However, how does H2A.Z regulate gene expression and histone modifications during preimplantation development remains unrevealed. Here, by using ultra‐low‐input native chromatin immunoprecipitation and sequencing, the genome‐wide distribution of H2A.Z is delineated in mouse oocytes and early embryos. These landscapes indicate that paternal H2A.Z is removed upon fertilization, followed by unbiased accumulation on parental genomes during zygotic genome activation (ZGA). Remarkably, H2A.Z exhibits hierarchical accumulation as different peak types at promoters: promoters with double H2A.Z peaks are colocalized with H3K4me3 and indicate transcriptional activation; promoters with a single H2A.Z peak are more likely to occupy bivalent marks (H3K4me3+H3K27me3) and indicate development gene suppression; promoters with no H2A.Z accumulation exhibit persisting gene silencing in early embryos. Moreover, H2A.Z depletion changes the enrichment of histone modifications and RNA polymerase II binding at promoters, resulting in abnormal gene expression and developmental arrest during lineage commitment. Furthermore, similar transcription and accumulation patterns between mouse and porcine embryos indicate that a dual role of H2A.Z in regulating the epigenome required for proper gene expression is conserved during mammalian preimplantation development.