bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Significance of Mesenchymal Circulating Tumor Cells in Patients With Oligometastatic Hormone-Sensitive Prostate Cancer Who Underwent Cytoreductive Radical Prostatectomy
Authors: Yang Guanjie , Xie Jun , Zhang Shun , Gu Wenyu , Yuan Jing , Wang Ruiliang , Guo Changcheng , Ye Lin , Peng Bo , Yao Xudong , Yang Bin 
Published: January 2022

PurposeGrowing evidence shows that circulating tumor cells (CTCs) become more aggressive after the epithelial–mesenchymal transition (EMT), though the clinical significance of CTCs undergoing EMT in oligometastatic hormone-sensitive prostate cancer (omHSPC) patients has not yet been reported. Accordingly, the aim of this study was to detect the CTC level and investigate the clinical significance of mesenchymal CTCs in omHSPC patients who underwent cytoreductive radical prostatectomy (CRP).Materials and MethodsBlood samples were drawn from 54 omHSPC patients who underwent CRP. The CanPatrol CTC enrichment technique was applied to isolate and identify different phenotypes of CTCs, which were classified as epithelial (E-CTCs), mesenchymal (M-CTCs), or biphenotypic epithelial/mesenchymal (Bi-CTCs). Univariable and multivariable Cox regression analyses were employed to investigate potential prognostic factors for metastatic castration-resistant prostate cancer (mCRPC)-free survival and cancer-specific survival (CSS). The prognostic value of CTCs for CSS and mCRPC-free survival was assessed using time-dependent receiver operating characteristic (ROC) curves and Kaplan–Meier analysis.ResultsCTCs were detected in 51 of 54 patients (94%). E-CTC, M-CTC, and Bi-CTC detection rates were 56%, 67%, and 85%, respectively. A positive correlation was found between the M-CTC count and number of bone metastases (p = 0.012). Time-dependent ROC analysis showed that the M-CTC count had higher predictive power than E-CTC or Bi-CTC for mCRPC-free survival (3-year area under the curve [AUC] values: 0.64, 0.60, and 0.61) and CSS (3-year AUC: 0.86, 0.58, and 0.67). Additionally, time-dependent ROC analysis revealed total CTCs (T-CTCs) ≥5 and M-CTCs ≥2 to be the cutoff points with optimal specificity and sensitivity. Based on multivariable Cox regression, T-CTC and M-CTC counts were both independently associated with CSS and mCRPC-free survival (all p &lt; 0.05), though E-CTCs and Bi-CTCs had no significant prognostic value (all p &gt; 0.05). Patients with T-CTC ≥5 or M-CTC ≥2 had significantly worse mCRPC-free survival and CSS than those with T-CTC&lt;5 or M-CTC&lt;2 (all p &lt; 0.05) after CRP.ConclusionCTC quantification and phenotype characterization provide prognostic information, and M-CTCs can be used as a novel biomarker for omHSPC patients who undergo CRP. The results need to be validated in prospective studies.
Clinical Spectrum and Burden of Influenza-Associated Neurological Complications in Hospitalised Paediatric Patients
Authors: Yu Michael Kwan Leung , Leung Cherry Pui Pik , Wong Wilfred Hing Sang , Ho Alvin Chi Chung , Chiu Annie Ting Gee , Zhi Helen Hui , Chan Godfrey Chi Fung , Chan Sophelia Hoi Shan 
Published: January 2022

BackgroundInfluenza is one of the most common causes of acute respiratory tract infections around the world. Influenza viruses can cause seasonal epidemics. There remains limited information on the impact of both seasonal influenza A and influenza B related hospitalisations from neurological complications in paediatric populations in Asia.ObjectivesTo examine both the clinical spectrum and healthcare burden of influenza-associated neurological complications (IANCs) within the paediatric population of Hong Kong.MethodsWe conducted a population-based retrospective study to identify all paediatric patients (&lt;18 years) admitted to a public hospital in Hong Kong with a confirmed influenza A or B infection between 2014 and 2018 using the Clinical Data Analysis and Reporting System of the Hospital Authority. The clinical spectrum of the paediatric patients with IANCs was studied. The clinical burden of paediatric influenza patients with IANCs were compared to paediatric influenza patients without neurological complications.ResultsA total of 28,016 children admitted to the paediatric wards diagnosed to have influenza A or B infection were identified, accounting for 5.7% (28,016/489,955) of total paediatric admissions. 67.3% had influenza A and 32.7% had influenza B, and 8.9% had IANCs. The mean annual incidence of IANCs in children was 57 per 100,000 population. The spectrum of IANCs in our paediatric patients included febrile seizures (80.6%), myositis (11.4%), seizures with fever (5.4%), influenza-associated encephalitis/encephalopathy (IAE) (2.6%) and rarely Guillain–Barré syndrome (0.04%). Most paediatric patients with IANCs (85.5%) presented at a young age of &lt;6 years. Paediatric patients with IANCs had significant longer hospital stays (p &lt; 0.001), higher percentages of mechanical ventilation use (p &lt; 0.05) and PICU admissions (p &lt; 0.001), and higher mortality rates (p &lt; 0.001) compared to those without neurological complications. Amongst those with IANCs, IAE was the sole cause of all seven reported mortalities.ConclusionsSeasonal influenza A &amp; B is a common cause of hospitalisation for paediatric patients in Hong Kong. We found neurological complications from influenza A and B caused a significantly higher clinical burden compared to those without neurological complications. Children in younger age groups (&lt;6 years old) are at highest risk and thus increasing vaccination coverage to this age group is recommended.
Step-Up Approach for Sodium Butyrate Treatment in Children With Congenital Chloride Diarrhea
Authors: Di Meglio Lavinia , Grimaldi Giusi , Esposito Francesco , Gelzo Monica , Esposito Maria Valeria , Castaldo Giuseppe , Canani Roberto Berni 
Published: January 2022

ObjectivesOral salt substitutive therapy is pivotal for the survival of patients with congenital chloride diarrhea (CLD), however this therapy is unable to influence the symptoms severity. Butyrate has been proposed to limit diarrhea severity in CLD. Unfortunately, the optimal dose schedule is still largely undefined. In addition, butyrate seems not to be well-tolerated by all patients, with some subjects reporting diarrhea worsening. We investigated the efficacy of a step-up therapeutic approach with sodium butyrate in patients who experienced a diarrhea worsening or an absent improvement after the direct administration of 100 mg/kg/day of sodium butyrate.MethodsThe efficacy of a step-up therapeutic approach starting from 50 mg/Kg/day with a subsequent 25 mg/kg/day weekly increase up to 100 mg/kg/day of oral sodium butyrate was investigated in previously three unresponsive CLD children.ResultsThe step-up therapeutic approach resulted effective in limiting diarrhea severity in all our three previously unresponsive CLD patients.ConclusionsOur results suggest the efficacy of the step-up therapeutic approach in CLD children.
Acid-Sensing Ion Channel 1/Calpain1 Activation Impedes Macrophage ATP-Binding Cassette Protein A1-Mediated Cholesterol Efflux Induced by Extracellular Acidification
Authors: Wang Yuan-Mei , Tan Mo-Ye , Zhang Rong-Jie , Qiu Ming-Yue , Fu You-Sheng , Xie Xue-Jiao , Gu Hong-Feng 
Published: January 2022

BackgroundExtracellular acidification is a common feature of atherosclerotic lesions, and such an acidic microenvironment impedes ATP-binding cassette transporter A1 (ABCA1)-mediated cholesterol efflux and promotes atherogenesis. However, the underlying mechanism is still unclear. Acid-sensing ion channel 1 (ASIC1) is a critical H+ receptor, which is responsible for the perception and transduction of extracellular acidification signals.AimIn this study, we explored whether or how ASIC1 influences extracellular acidification-induced ABCA1-mediated cholesterol efflux from macrophage-derived foam cells.MethodsRAW 264.7 macrophages were cultured in an acidic medium (pH 6.5) to generate foam cells. Then the intracellular lipid deposition, cholesterol efflux, and ASIC1/calpain1/ABCA1 expressions were evaluated.ResultsWe showed that extracellular acidification enhanced ASIC1 expression and translocation, promoted calpain1 expression and lipid accumulation, and decreased ABCA1 protein expression as well as ABCA1-mediated cholesterol efflux. Of note, inhibiting ASIC1 activation with amiloride or Psalmotoxin 1 (PcTx-1) not only lowered calpain1 protein level and lipid accumulation but also enhanced ABCA1 protein levels and ABCA1-mediated cholesterol efflux of macrophages under extracellular acidification conditions. Furthermore, similar results were observed in macrophages treated with calpain1 inhibitor PD150606.ConclusionExtracellular acidification declines cholesterol efflux via activating ASIC1 to promote calpain1-mediated ABCA1 degradation. Thus, ASIC1 may be a novel therapeutic target for atherosclerosis.
Resilience and Posttraumatic Growth of Patients With Breast Cancer During the COVID-19 Pandemic in China: The Mediating Effect of Recovery
Authors: Shi Jing , Sznajder Kristin K. , Liu Shuo , Xie Xinyue , Yang Xiaoshi , Zheng Zhen 
Published: January 2022

PurposeThis study aims to examine the mediating role recovery plays in the relationship between resilience and posttraumatic growth (PTG) among breast cancer patients.MethodsA cross-sectional study design was implemented between January 02, 2021 and April 29, 2021. A total of 789 breast cancer patients from eight hospitals in Liaoning province were selected for participation in this study. These participants completed questionnaires, which included the Post-Traumatic Growth Inventory, EGO Resilience Scale and the Questionnaire about the Process of Recovery. The associated factors of PTG were analyzed using hierarchical multiple regression (HMR). The proposed relationships among resilience, recovery, and PTG were checked by structural equation modeling (SEM) analyses.ResultsThe average PTG score of breast cancer patients was 53.00 ± 28.30. PTG was positively correlated with both recovery and PTG (a*b = 0.1, BCa95% CI: 0.154 ∼ 0.054).ConclusionBreast cancer patients were found to exhibit a moderate degree of PTG. Resilience was positively associated with PTG and recovery mediated the positive effect of resilience on PTG. Resilience might serve as a crucial protective factor that could explain positive growth in life-threatening illnesses through the mediating path of recovery.
Birth Experience Mediates the Association Between Fear of Childbirth and Mother-Child-Bonding Up to 14 Months Postpartum: Findings From the Prospective Cohort Study DREAM
Authors: Seefeld Lara , Weise Victoria , Kopp Marie , Knappe Susanne , Garthus-Niegel Susan 
Published: January 2022

ObjectiveTo explore the longitudinal associations between prepartum fear of childbirth (FOC), birth experience, and postpartum mother-child-bonding, and the potential mediator role of the birth experience.DesignWomen from the prospective cohort study DREAM completed questionnaires during pregnancy, 8 weeks, and 14 months after the birth.ParticipantsA community sample of n = 645 pregnant women from a large city in Eastern Germany participated in the study.ResultsIn a regression analysis, FOC predicted negative birth experience (β = 0.208, p &lt; 0.001) which in turn predicted poorer mother-child-bonding both at 8 weeks (β = 0.312, p &lt; 0.001) and 14 months postpartum (β = 0.200, p &lt; 0.001). FOC also predicted mother-child-bonding at 14 months postpartum (β = 0.098, p &lt; 0.05). Of note, this association was mediated by birth experience both at 8 weeks, indirect effect ab = 0.065, 95% CI [0.036, 0.098], and 14 months postpartum, indirect effect ab = 0.043, 95% CI [0.023, 0.067]. These effects remained stable even when adjusting for potential confounders.Key ConclusionsThis study suggests that the association between FOC and mother-child-bonding is mediated by birth experience, pointing to the importance of a woman's positive subjective experience.Implications for PracticeFindings reveal two targets for peripartum interventions for women at risk for poor mother-child-bonding, namely the implementation of FOC screenings during pregnancy, and birth experience as mediating factor between FOC and mother-child-bonding. Focusing on the mother's subjective birth experience could aid to identify women at risk for impaired bonding who might need additional support.
The Effect of Indoor Daylight Levels on Hospital Costs and Length of Stay of Patients Admitted to General Surgery
Authors: Li Xiawei , Lou Jianyao , Yuan Zheping , Shi Aiguang , Wang Ning , Zhou Lin , Zhao Mingchen , Ye Fanghe , Pan Zikun , Wu Yulian 
Published: January 2022

BackgroundIndoor daylight levels can directly affect the physical and psychological state of people. However, the effect of indoor daylight levels on the clinical recovery process of the patient remains controversial. This study was to evaluate the effect of indoor daylight levels on hospital costs and the average length of stay (LOS) of a large patient population in general surgery wards.MethodsData were collected retrospectively and analyzed of patients in the Second Affiliated Hospital of Zhejiang University, School of Medicine between January 2015 and August 2020. We measured daylight levels in the patient rooms of general surgery and assessed their association with the total hospital costs and LOS of the patients.ResultsA total of 2,998 patients were included in this study with 1,478 each assigned to two daylight level groups after matching. Overall comparison of hospital total costs and LOS among patients according to daylight levels did not show a significant difference. Subgroup analysis showed when exposed to higher intensity of indoor daylight, illiterate patients had lower total hospital costs (CNY ¥13070.0 vs. ¥15210.3, p = 0.018) and shorter LOS (7 vs. 10 days, p = 0.011) as compared to those exposed to a lower intensity.ConclusionsIndoor daylight levels were not associated with the hospital costs and LOS of patients in the wards of general surgery, except for those who were illiterate. It might be essential to design guidelines for medical staff and healthcare facilities to enhance the indoor environmental benefits of daylight for some specific populations.
Longitudinal Patterns of Food Procurement Over the Course of the COVID-19 Pandemic: Findings From a Canadian Online Household Survey
Authors: Nielsen Daiva E. , Labonté Katherine , Karamanoglu Irem , Han Hannah Yang , Tavanaei Mandana , Duhamel Paul-Guy , Agellon Luis B. , Paquet Catherine , Dube Laurette 
Published: January 2022

IntroductionConsumer food procurement during the COVID-19 pandemic has been understudied. This investigation aimed to longitudinally evaluate food procurement patterns, concern of virus exposure in grocery retailers, and food access challenges over the pandemic among a sample of households in Quebec, Canada.MethodsOnline surveys were collected at three time points of the pandemic: first wave in spring 2020 (lockdown period), summer 2020 (deconfinement period), and second wave in winter 2021 (curfew period). Respondents were the household's primary grocery shopper (n = 491). Non-parametric tests and multivariable logistic regression were conducted to compare responses over time and to evaluate characteristics of respondents who regularly used no-contact grocery methods (store pick-up or home delivery).ResultsFrequency of in-store grocery shopping was lowest during the lockdown (once per week or less), and significantly increased over time to resemble pre-pandemic frequency. Concern of virus exposure in grocery retailers and disinfection/discarding of food packaging was highest during the lockdown, but significantly decreased over time. At all time points, use of public transit, walking or cycling for grocery shopping was associated with regular use of no-contact grocery methods (curfew odds ratio (OR): 3.13 (95% confidence interval 1.60, 6.14). Age (60 years+) was associated with regular use during the lockdown [OR: 2.27 (1.13, 4.59)].ConclusionAmong our sample, frequency of in-store grocery shopping was lowest and concern of virus exposure in stores was highest during the lockdown period. No-contact grocery use was associated with transportation mode and potentially with personal risk perception (age).
Evaluation of Bone Wax Coated Bipolar Coagulation Forceps: Performance and Safety Assessment
Authors: Shi Jichun , Wei Wei , Wang Zhen , Ren Haobin , Jia Chenguang , Dong Lixin , Li Zhengwei , Zhang Jianjian , Feng Yu , Huang Kaixin , Li Xiang , Chen Jincao 
Published: January 2022

BackgroundImproving the performance of bipolar coagulation forceps is crucial for safer and more accurate neurosurgery. In our department, we found that bone wax (BW) melted by thermal effect of bipolar electrocoagulation can achieve more efficient hemostasis and reduce the amount of BW in neurosurgical procedures associated with bleeding from emissary and diploic veins. Nevertheless, relevant studies are still lacking to verify our finding.ObjectiveThe study objectives were to evaluate the performance and safety in electrocoagulation: (1) compare the performance of BW coated bipolar coagulation forceps and the conventional anti-stick forceps in vivo, and (2) assess the safety of electrocoagulation with BW coated bipolar coagulation forceps in rat primary motor cortex.MethodsTissue adhesion was evaluated by comparing the wetting tension and the amount of protein adhered to the forceps tips after electrocoagulation. Thermal damage was assessed by analyzing the thermography and H&amp;E staining of coagulated rat brain tissues. The hemostatic efficiency was reflected by the number of electrocoagulation until complete hemostasis and the condition of damaged common carotid arteries. The safety of BW coated forceps in electrocoagulation was assessed by evaluating the inflammation of coagulated rat primary motor cortex and the motor functions at the 7th day postoperatively.ResultsBone wax coated forceps had a significantly higher contact angle and adhered less coagulum. Thermography was acquired at 3 s, 6 W units in rat primary motor cortex in vivo. The highest temperature recorded during BW coated tips application was significantly lower than the uncoated. In addition, there was a relatively smaller tissue injury area produced by the BW coated forceps. Additionally, BW coated forceps improved the hemostatic efficiency and caused fewer injuries on the damaged arteries (3 s, 10 W units). More importantly, electrocoagulation with BW coated forceps led to no significant motor function impairments and less glial and microglia responses.ConclusionThis study reveals that BW coated bipolar coagulation forceps can provide a convenient, cost-efficient, safer, and more efficient way for hemostasis. More research is needed to evaluate the electrocoagulation with BW in the long term and verify our finding in human beings.
Association Between Preoperative Blood Glucose Level and Hospital Length of Stay in Patients With Kidney Stones Undergoing Percutaneous Nephrolithotomy
Authors: Sun Si , Mao Weipu , Tao Shuchun , Wan Lilin , Zou Xiangyu , Zhang Guangyuan , Chen Ming 
Published: January 2022

AimTo assess the effect of preoperative blood glucose (POBG) levels on the length of stay (LOS) in patients with kidney stones undergoing percutaneous nephrolithotomy (PCNL).MethodsWe conducted a retrospective study of patients who underwent PCNL at the Zhongda Hospital of Southeast University from 2013 to 2019. The relationship between POBG level and LOS was investigated by dose-response analysis curves of restricted cubic spline function.ResultsWe included 310 patients and divided them into three groups (&lt;5.04, 5.04 to &lt;6.88, ≥6.88 mmol/L) according to the POBG levels. Patients with POBG levels ≥6.88 mmol/L (adjusted odds risk [aOR] 1.67; 95% CI 0.83–3.33) had a 67% higher risk of LOS &gt; 2 weeks than patients with POBG levels &lt;5.04 mmol/L. A positive dose-response analysis curve was observed between POBG and the adjusted risk of LOS &gt;2 weeks. Similar results were observed in the subgroups analysis.ConclusionWe demonstrated that higher POBG levels were significantly associated with longer LOS in patients with kidney stones undergoing PCNL.
Length Scale Analyses of Background Error Covariances for EnKF and EnSRF Data Assimilation
Authors: Park Soon-Young , Dash Uzzal Kumar , Yu Jinhyeok 
Published: January 2022

Data assimilation (DA) combines incomplete background values obtained via chemical transport model predictions with observational information. Several 3-Dimensional variational (3DVAR) and sequential methods (e.g., ensemble Kalman filter (EnKF)) are used to define model errors and build a background error covariance (BEC) and are important factors affecting the prediction performance of DA. The BEC determines the spatial range, where observation concentration is reflected in the model when DA is applied to an air pollution transport model. However, studies investigating the characteristics of BEC using air quality models remain lacking. In this study, horizontal length scale (HLS) and vertical length scale (VLS) analyses of a BEC were applied to EnKF and ensemble square root filter (EnSRF), respectively, and two ensemble-based DA methods were performed; the characteristics were compared with those of a BEC applied to 3DVAR. The results of 6 h PM2.5 predictions performed for 42 days were evaluated for a control run without DA (CTR), 3DVAR, EnKF, and EnSRF. HLS and VLS respectively exhibited a high correlation with the ground wind speed and with the planetary boundary layer height for diurnal and daily variations; EnKF and EnSRF exhibited superior performances among all the methods. The root mean square errors were 11.9 μg m−3 and 11.7 μg m−3 for EnKF and EnSRF, respectively, while those for 3DVAR and CTR were 12.6 μg m−3 and 18.3 μg m−3, respectively. Thus, we proposed a simple method to find a Gaussian function that best described the error correlation of the BEC based on the physical distance.
ANALISIS FAKTOR YANG MEMPENGARUHI KEPUASAN KONSUMEN PADA PEMBELIAN SAWI ORGANIK DI FARMERS MARKET SEMARANG
Authors: Hermansyah Dimas , Roessali Wiludjeng , Prasetyo Edy 
Published: January 2022

Sawi merupakan satu diantara sayuran organik yang dijual di Farmers Market. Kepuasan konsumen menjadi poin penting yang perlu diperhatikan oleh Farmers Market untuk meningkatkan kinerjanya. Penelitian ini bertujuan untuk menganalisis:1). Kepuasan konsumen pada pembelian sawi organik di Farmers Market 2). Faktor-faktor yang mempengaruhi kepuasan konsumen dalam pembelian sawi organik di Farmers Market. Penelitian ini dilaksanakan pada bulan Mei hingga Juni 2021 di Farmers Market Semarang. Penelitian menggunakan metode survey. Metode pengambilan sampel yang digunakan yaitu accidental sampling. Data diperoleh dari hasil wawancara dengan responden menggunakan kuesioner yang terlah disiapkan. Jumlah sampel yang digunakan didapatkan dari lima kali jumlah butir pertanyaan yang telah dibuat yaitu sebanyak 100 responden. Kriteria responden yang dipilih adalah konsumen yang pernah melakukan pembelian sawi organik serta bersedia diwawancara dan berusia 20-45 tahun. Analisis data menggunakan metode kuantitatif meliputi Importance Performance Analysis (IPA), Customer Satisfaction Index (CSI) dan Regresi Logistik. Hasil perhitungan IPA diketahui atribut promosi masih perlu ditingkatkan untuk memenuhi harapan konsumen sedangkan atribut produk, harga dan kualitas pelayanan menjadi prioritas utama dan perlu dipertahankan karena sudah sesuai dengan harapan konsumen Farmers Market. Perhitungan CSI didapatkan nilai 79,52% yang menunjukkan konsumen puas terhadap sayur organik yang dijual. Hasil dari perhitungan Regresi Logistik menunjukkan bahwa variable yang berpengaruh terhadap kepuasan konsumen adalah produk, harga dan kualitas pelayanan, sedangkan promosi tidak menunjukkan pengaruh signifikan