bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting epigenetic features in clear cell sarcomas based on patient-derived cell lines
Authors: Karner Christina , Anders Ines , Vejzovic Djenana , Szkandera Joanna , Scheipl Susanne , Deutsch Alexander J A , Weiss Larissa , Vierlinger Klemens , Kolb Dagmar , Kühberger Stefan , Heitzer Ellen , Habisch Hansjörg , Zhang Fangrong , Madl Tobias , Reininger-Gutmann Birgit , Liegl-Atzwanger Bernadette , Rinner Beate 
Published: November 2022

Abstract
Background: Clear cell sarcomas (CCSs) are translocated aggressive malignancies, most commonly affecting young adults with a high incidence of metastasis and poor prognosis. Research into the disease is more feasible when adequate models are available. By establishing CCS cell lines from a primary and metastatic lesion and isolating healthy fibroblasts from the same patient, the in vivo process is accurately reflected and aspects of clinical multistep carcinogenesis recapitulated.
Methods: Isolated tumor cells and normal healthy skin fibroblasts from the same patient were compared in terms of growth behavior and morphological characteristics using light and electron microscopy. Tumorigenicity potential was determined by soft agar colony formation assay and in vivo xenograft applications. While genetic differences between the two lineages were examined by copy number variation profiles, nuclear magnetic resonance spectroscopy determined arginine methylation as epigenetic features. Potential anti-tumor effects of a protein arginine N-methyltransferase type I (PRMT1) inhibitor were elicited in 2D and 3D cell culture experiments using cell viability and apoptosis assays. Statistical significance was calculated by one-way ANOVA and unpaired t-test.
Results: The two established CCS cell lines named MUG Lucifer prim and MUG Lucifer met showed differences in morphology, genetic and epigenetic data, reflecting the respective original tissue. The detailed cell line characterization especially in regards to the epigenetic domain allows investigation of new innovative therapies. Based on the epigenetic data, a PRMT1 inhibitor was used to demonstrate the targeted antitumor effect; normal tissue cells isolated and immortalized from the same patient were not affected with the IC50 used.
Conclusions: MUG Lucifer prim, MUG Lucifer met and isolated and immortalized fibroblasts from the same patient represent an ideal in vitro model to explore the biology of CCS. Based on this cell culture model, novel therapies could be tested in the form of PRMT1 inhibitors, which drive tumor cells into apoptosis, but show no effect on fibroblasts, further supporting their potential as promising treatment options in the combat against CCS. The data substantiate the importance of tailored therapies in advanced metastatic stage of CCS.
Longitudinal surveillance of serum titanium ion levels in patients with indigenous 3D printed total temporomandibular joint replacement
Authors: Khandelwal Garima , Roychoudhury Ajoy , Bhutia Ongkila , Shariff A. 
Published: November 2022

Abstract
Objective: The purpose of this longitudinal study was to surveil the serum titanium ion levels at various time intervals in patients with indigenous 3D-printed total temporomandibular joint replacement (TMJ TJR).
Study design: The study was conducted on 11 patients (male: 8; female: 3) who had undergone unilateral or bilateral TMJ TJR. Blood samples were drawn preoperatively (T0), 3 months (T1), 6 months (T2), and 1 year (T3) postoperatively. Data were analyzed and a p value of <0.05 was considered statistically significant.
Results: The mean serum titanium ion levels at T0, T1, T2, and T3 was 9.34 ± 8.70 microgram/L (mcg/L), 35.97 ± 20.27 mcg/L, 31.68 ± 17.03 mcg/L, and 47.91 ± 15.47 mcg/L respectively. The mean serum titanium ion levels increased significantly at T1 (p= 0.009), T2 (p= 0.032), and T3 (p=0.00) interval. There was no significant difference between unilateral and bilateral groups.
Conclusion: Serum titanium ion continued to show increased levels till the last follow-up of 1 year. These initial serum titanium ion levels increase is due to the initial wear phase of the prosthesis which manifests over 1 year. Further studies with large sample sizes and long-term follow-ups are required to see the deleterious effect if any on the TMJ TJR.
The Effect of Student Fatigue on Teaching Quality: A Prospective Cohort Study Based on One University
Authors: xiaoqun Qin , ye Chen 
Published: November 2022

Abstract
Students' fatigue is a serious problem which affects the quality of teaching and the physical and mental health of students and must be paid attention to by the whole society and medical workers. This study was a prospective cohort study of fatigue related problems among computer science majors at a university. With reference to "Fatigue scale-14", combining with the learning characteristics of students in Chinese education system and applying the statistical principle, the "Fatigue Scale-18" was developed. In spring, summer, autumn and winter, 319 and 1276 college students were monitored for fatigue incidence, fatigue degree and gender differences. The incidence of fatigue was 64.97%, 70.73% in boys and 61.46% in girls. Psychological fatigue accounted for 79.33% and physiological fatigue accounted for 20.67%. The main causes of fatigue were analyzed. The influence of fatigue on teaching effect is empirically studied, and the influence of fatigue on learning of students with different learning foundations is studied. Effects of fatigue incidence in different climates on learning outcomes. Based on the above research, the quantitative relationship between academic performance and fatigue degree and gender was listed as follows: academic performance =90.208—2.906× fatigue grade—4.380× gender (boy 1, girl 2). Corresponding preventive measures are put forward in view of the causes of fatigue among college students, and it is suggested that the incidence of fatigue should be controlled below 30%; it is proposed that college education should pay attention to student fatigue, monitor students' fatigue status regularly or irregularly, and take corresponding preventive measures for the causes of fatigue. Reduce the impact of student fatigue on learning and health. It provides theoretical and empirical basis for preventing students' fatigue, improving teaching quality and ensuring students' health.
Endoscopic papillary balloon dilation can be safely performed in patients with dual antiplatelet therapy: A pilot study
Authors: Masuda Sakue , Jinushi Ryuhei , Koizumi Kazuya , Makazu Makomo , Nishino Takashi , Shionoya Kento , Kimura Karen , Sumida Chihiro , Kubota Jun , Ichita Chikamasa , Sasaki Akiko , Kobayashi Masahiro , Kako Makoto , Uojima Haruki , Sugitani Ayumu 
Published: November 2022

Abstract
Endoscopic papillary balloon dilation (EPBD)—a low-risk procedure for bleeding—has been suggested as an alternative to endoscopic sphincterotomy (EST) for papillary dilatation in patients undergoing choledocholithotomy and at a high risk of bleeding. Several guidelines recommend dual antiplatelet therapy (DAPT) be reduced to single antiplatelet therapy (SAPT) when EST is performed. However, there is no evidence that EPBD increases the risk of bleeding in patients receiving DAPT. Thus, we aimed to address this problem. We included 31 patients who underwent EPBD for CBD stones and received DAPT or SAPT prior to EPBD (9 in the DAPT group and 22 in the SAPT group) treated at our hospital from May 2014 to August 2022. The DAPT group included patients who underwent EPBD without antiplatelet therapy withdrawal or with a shorter withdrawal period than those recommended by the guidelines. In the DAPT group, one of the two antiplatelet agents used was thienopyridine. No bleeding was observed after EPBD in this study. We did not find any significant between-group differences in the change in hemoglobin levels and post-ERCP pancreatitis. Thus, we propose that EPBD does not increase the bleedingrisk in patients with DAPT.
Novel green fluorescent probe stem from carbon quantum dots for specific recognition of tyrosinase in serum and living cells
Authors: Wang Wenxian , Yang Dan , Zhou Yufei , Zhang Yuding , Guan Lijiao , Zhang Xianfen , Xue Weiming , Huang Saipeng 
Published: November 2022

Abstract
Tyrosinase (TYR), an important biomarker for melanoma, offered significant information early detection of melanoma and may decrease the likelihood of mortality. Therefore, this article constructed a highly sensitive and selective green fluorescent functionalized carbon quantum dots (TYR-CQDs) for tyrosinase (TYR) activity detection by one-step hydrothermal protocol utilizing catechol, citric acid and urea as precursors. The prepared TYR-CQDs illustrated excellent linear relationship and broad linear range with a low detection limit, which exhibited high accuracy and recovery in quantitative determination of TYR in human serum samples. Furthermore, the TYR-CQDs had successfully realized intracellular TYR detection owing to excellent biocompatibility, high anti-interference ability and good cellular imaging capability, suggesting the potential biomedical applications in early diagnosis of melanoma and other tyrosinase-related diseases.
A primate-specific (CCG) repeat in DISP2 is subject to natural selection in human and harbors unambiguous genotypes in late-onset neurocognitive disorder
Authors: Khamse S , Alizadeh S , SH Bernhart , Afshar H , Delbari A , Ohadi M 
Published: November 2022

Abstract

Intact blocks of (CCG)-repeats are among the top short tandem repeats (STRs), which have undergone natural selection. The above stems from the facts that these STRs are mutation hotspots for C to T truncating substitutions, and are predominantly enriched in the exons. The humanDISP2(dispatched RND transporter family member 2) has the highest level of expression in the brain, and contains a (CCG)-repeat at the interval between + 1 and + 60 of the transcription start site (ENST00000267889.5 DISP2-201), which ranks in the top 1 percent of (CCG) STRs in respect of length. Here we sequenced this STR in a sample of 448 Iranian individuals, consisting of late-onset NCDs (N = 203) and controls (N = 245). While the region spanning the (CCG)-repeat was highly mutated and contained several C to T transitions, which resulted in several (CCG)-residues, a 8-repeat of the (CCG)-STR was the predominantly abundant allele (frequency = 0.92) across the two groups. The overall distribution of alleles was not different between the two groups (p > 0.05). However, we detected four genotypes that belonged to the NCD group only (2% of the NCD genotypes, Mid-p = 0.02), and consisted of allele lengths that were not detected in the control group. We also found six genotypes that were detected in the control group only (2.5% of the control genotypes, Mid p = 0.01). While the group-specific genotypes formed a small percentage of the overall genotypes, they unveil an underappreciated feature, in which complex disorders such as late-onset NCDs may be linked with unambiguous genotypes.
High-harmonic generation in liquids with few-cycle pulses: effect of laser-pulse duration on the cut-off energy
Authors: Wörner Hans Jakob , Mondal Angana , Waser Benedikt , Balciunas Tadas , Yin Zhong , Neufeld Ofer , Tancogne-Dejean Nicolas , Rubio Angel 
Published: November 2022

Abstract
High-harmonic generation (HHG) in liquids is opening new opportunities for attosecond light sources and attosecond time-resolved studies of dynamics in the liquid phase. In gas-phase HHG, few-cycle pulses are routinely used to create isolated attosecond pulses and to extend the cut-off energy. Here, we study the properties of HHG in liquids, including water and several alcohols, by continuously tuning the pulse duration of a mid-infrared driver from the multi- to the sub-two-cycle regime. Similar to the gas phase, we observe the transition from discrete odd-order harmonics to continuous extreme-ultraviolet emission. However, the cut-off energy is shown to be entirely independent of the pulse duration. This observation is confirmed by ab-initio simulations of HHG in large clusters. Our results support the notion that the cut-off energy is a fundamental property of the liquid, independent of the driving-pulse properties. Combined with the recently reported wavelength-independence of the cutoff, these results confirm the direct sensitivity of HHG to the mean-free paths of slow electrons in liquids. Our results additionally imply that few-cycle mid-infrared laser pulses are suitable drivers for generating isolated attosecond pulses from liquids.
Nimbolide targets multiple signalling pathways to reduce neuroinflammation in BV2 microglia
Authors: Katola Folashade O , Olajide Olumayokun A 
Published: November 2022

Abstract
Nimbolide, a limonoid compound found in the neem plant, was investigated for effects on neuroinflammation in BV2 microglia activated with LPS. Cultured BV2 cells were stimulated with LPS (100 ng/ml) followed by treatment with nimbolide (125, 250 and 500 nM). Results showed that nimbolide caused significant reduction in the levels of TNFα, IL-6, IFNγ, NO/iNOS, PGE2/COX-2 in LPS-activated BV2 cells. Further experiments revealed that LPS-induced increased expression of phospho-p65 and phospho-IκBα proteins were reduced in the presence of nimbolide. Also, LPS-induced NF-κB acetylation, increased binding to consensus sites, and transactivation, as well as phosphorylation of p38 and JNK MAPKs were reduced by nimbolide. Reduction of cellular ROS generation by nimbolide was accompanied by reduction in gp91phox protein levels, while antioxidant effects were observed through elevation in protein levels of HO-1 and NQO-1. It was observed that treatment of BV2 microglia with nimbolide resulted in reduced levels of cytoplasmic Nrf2 which was accompanied by increased levels in the nucleus. Furthermore, treatment with this compound resulted in increased binding of Nrf2 to ARE consensus sites accompanied by enhanced ARE luciferase activity. Knockdown experiments revealed a loss of anti-inflammatory activity by nimbolide in cells transfected with Nrf2 siRNA. Treatment with nimbolide resulted in nuclear accumulation of SIRT-1, while siRNA knockdown of SIRT-1 resulted in reversal of anti-inflammatory activity of nimbolide. It is proposed that nimbolide reduces neuroinflammation in BV2 microglia through mechanisms resulting in dual inhibition of NF-κB and MAPK pathways. It is also proposed that activation of Nrf2 antioxidant as well as SIRT-1 deacetylation mechanisms may be contributing to its anti-inflammatory activity.
Epidemiological and phylogenetic characterization of unusual P[14] rotavirus strains in hospitalized children with acute gastroenteritis
Authors: Tatsi Elizabeth Barbara , Koukou Dimitra-Maria , Dellis Charilaos , Dourdouna Maria-Myrto , Michos Athanasios , Syriopoulou Vasiliki 
Published: November 2022

Abstract
Rotavirus A (RVA) is characterized by molecular and epidemiological diversity. To date, 42G and 58P RVA genotypes have been identified, some of which have zoonotic origin, like P[14]. This study aims at the epidemiological and molecular characterization of human P[14] RVA. Fecal samples from children ≤ 16 years with acute gastroenteritis (AGE), hospitalized during 01/2007-12/2021, were tested for RVA by chromatographic immunoassay. Demographic, clinical and laboratory data were recorded. Positive RVA samples were G and P typed performing Sanger sequencing. Phylogenetic analysis of P[14] was performed using the Mega X software. Sixty (1.36%) out of 4427 children with RVA AGE, were infected with an unusual G (G6/G8/G10) or P (P[6]/P[9]/P[10]/P[11]/P[14]) genotype. P[9] (27/60; 45%) was the most common unusual genotype followed by P[14] (11/60; 18.3%). Median age of children with P[14] was 37.8 months (IQR:17.6–77.1), 6/11 were males and 4/11 resided to rural areas. Their symptoms were diarrhea (9/11; 81.8%), vomiting (7/11; 63.6%), fever (7/11; 63.6%) and moderate dehydration (6/11; 54.5%). All children were unvaccinated for RVA. Seasonal peak of P[14] was during spring (91%). The combinations G8P[14] (45.5%), G6P[14] (36.4%) and G4P[14] (18.1%) were detected. Phylogenetic analysis showed potential evolutionary relationship of three human RVA P[14] with a fox strain from Croatia. These findings enhance the potential zoonotic origin of P[14] and the interspecies transmission between nondomestic animals and humans, which may lead to new RVA genotypes with unknown severity.
An integrated high-density genetic linkage map of tobacco (Nicotiana tabacum L.)
Authors: Shen Yang , Wu Chenglin , Cheng Junqi , Li Yapei , Lin Zhongxu , Cao Jinglin 
Published: November 2022

Abstract
Tobacco is an important non-food crop with many applications, which is widely cultivated worldwide. Genetic linkage maps play an important role in QTL mapping, gene targeting, etc. However, due to the narrow genetic background and large genome of tobacco, the research on the tobacco genetic map lags behind. At present, although several linkage maps have been constructed based on SSR (simple sequence repeat) markers, different SSR markers limit their widespread applications. In order to construct an integrated linkage map, we identified the redundancy of tobacco SSR markers, and constructed a high-quality genetic map by using the integration method based on four tobacco genetic maps. Finally, 20 redundant markers were found from 3,354 SSR markers, and the integrated high-density genetic linkage map (IHD) had a total of 3,377 loci with a length of 2,489.82 cM and a density of 1.36 locus/cM. The high-density integrated genetic map will have great application in the field of QTL mapping and marker-assisted breeding in tobacco.
Micro-RNA-93-5p and micro-RNA-3613-5p in exosomes released from vascular endothelial cells regulate medial arterial calcification in hemodialysis patients
Authors: Dongliang Zhang , Chen Fu , Chengai Wu , Ziye Chen 
Published: November 2022

Abstract
Objective:
To determine the underlying mechanism for how circulating exosomes with micro-RNA (Exo-miRNA) cargos promote medial arterial calcification (MAC) in maintenance hemodialysis (MHD) patients.
Methods and Results:
Plasma samples and fistular vascular tissues were collected from 12 MHD patients. Histological examinations and Exo-miRNA aberrant expression assays were conducted on the samples. The Exo-miRNAs in supernatants of human umbilical vein endothelial cells (HUVECs) treated with asymmetric dimethylarginine (ADMA) were compared with those in control HUVECs. Two similar miRNAs between patient plasma samples and cell culture supernatants, miR-93-5p and miR-3613-5p, were selected for cell culture experiments in vitro. Human aortic smooth muscle cells (HASMCs) were incubated with mimics of these miRNAs or cocultured with ADMA-treated HUVECs using a transwell system. Target proteins in the cell lysates were detected by western blotting. The histological examinations provided images of MAC Compared with control HASMCs, up-regulation of S100A11a, PI3K p110α, NF-κB, p38 MAPK, Rab11a, and F-actin was observed in the miR-93-5p mimic group, while up-regulation of Rab11a and F-actin was noted in the miR-3613-5p mimic group.
Conclusion:
MiRNA-93-5p and miRNA-3613-5p in exosomes released from vascular endothelial cells enter into vascular smooth muscle cells and regulate MAC in MHD patients.
Vulnerability of rice and maize yield in response to the climate and aerosol variability over India
Authors: Gupta Dilip Kumar , Pramanick Subhajit , Singh Abhay Kumar 
Published: November 2022

Abstract
The progressive accumulation of anthropogenic aerosol pollution alters the physical and chemical properties of the atmosphere, causes extreme variations in weather variables, and has severe negative repercussions on agriculture. The present study evaluates the time series analysis of crop production, crop cultivation area, weather, and aerosol variations over the time period of the years 1998 to 2019 for rice and maize crops in India. The impact of weather and aerosol variations is also evaluated on rice and maize yield. The increasing trend of rice and maize yield is found with a rate of 0.888 million tons/year and 0.561 million tons/year over the time period of 1998 to 2019, respectively, due to the use of more sophisticated agricultural practices, optimum fertiliser, and irrigation, climate-resilient varieties, etc. The time series increasing trend is observed for the average value of weather variables (TMAX, TMIN, rainfall, relative humidity) and aerosol optical depth (AOD). While the average value of surface soil moisture (SM) and a fraction of absorbed photosynthetically active radiation (FAPAR) is found to decresing the trend over the time period 1998 to 2019. The main findings of this study are that the rice and maize yield is reduced by about (2.55%, 2.92%, 2.778%, 4.84%, 2.90%, 2.82%) and (5.12%, 6.57%, 6.93%, 6.54%, 4.97%, and 5.84%) due to the unexceptional variations in weather variables, namely maximum and minimum temperature, rainfall, relative humidity, soil moisture, and FAPAR during the time period of 1998 to 2019, respectively. However, the rice and maize yields are reduced by 7.9% and 8.8%, respectively, due to the increment of aerosol pollution. Overall, the study brings out conclusive evidence of the negative impact on rice and maize yield due to the variability in weather/FAPAR/AOD.
A modified sequential kalman asynchronous track fusion based on sound propagation time
Authors: Sheng xueli , Wang Yan , Mu Mengfei , Song Lai , Wu Zeyi 
Published: November 2022

Abstract
Abstract： This paper presents a modified sequential kalman asynchronous track fusion method based on the sound propagation time. The proposed method deals with the asynchronous issue, which is caused by different acoustic signal propagation times, for the distributed underwater acoustic detection systems. When the bearing of the target is estimated by various sensors distributed system, we adopt the the effective sound velocity approach to calculate the sound propagation time from the target signals to the receving sensors respectively. We also update the tracked target information asynchronously for distributed detection system, using the multi-sensor asynchronous sampling information. The sequential kalman filtering algorithm is employed here to achieve the asynchronous track fusion. Simulation results demonstrate that the proposed algorithm can significantly improve the location accuracy compared with the original algorithm. The adaptability of the proposed method to the addition and deletion of the fusion sensor is proved robust and has a certain application value.