bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single use of cytokine-adsorption-membrane Oxiris® in the treatment of severe malaria with acute kidney injury: a case-report
Authors: Zuchen Flavia , Serafim Rodrigo , Oliveira Joan , Carvalho Paula Lacorte , Andrade Regis Mariano , Rocha Eduardo , Maccariello Elizabeth 
Published: August 2022

Abstract
Background
Malaria´s treatment improved throughout the last decades mainly focused on eradicating the plasmodium. Nevertheless, its severe form is still a threat to over half the world’s population leaving opportunities to evaluate new approaches in managing the cytokine storm often associated with these cases.
Case Presentation
A previously healthy 60 years-old male was hospitalized due to a prolonged high fever after a recent visit to the Equatorial Guinea. The Plasmodium Falciparum Malaria diagnosis was made and soon after hospital admission his clinical condition rapidly deteriorated with hemodynamic instability, need of mechanical ventilation and acute kidney injury. Continuous renal replacement therapy (CRRT) was initiated using the Oxiris® filter, an endotoxin and cytokines adsorption membrane currently used in our center in septic shock especially when due to gran negative bacteria. In the present case, the rational for its prescription was based on the similarity of sepsis and malaria cytokine storm in severe cases. A remarkable clinical improvement started within 24 hours after CRRT, followed by a clear decreasing in blood inflammatory mediators after 72 hours of its initiation (lactate: from 71 to 20 mg/dL; procalcitonin: from 73.9 to 33ng/L, interleukin-6: from 590 to 106 pg/mL). Seven days after admission, the patient acquired a ventilator-ssociated pneumonia, and targeted antimicrobial treatment was started. Because of multiple complications related to long in-hospital stay, patient remained hospitalized for five months before being discharged. He recovered renal function and no longer needed renal replacement therapy after discharge.
Conclusions
There are no previous or ongoing trials regarding the use of endotoxins and cytokines adsorbing membranes in the setting of severe malaria. The prompt clinical and laboratory improvement after the initiation of blood purification suggests cytokine adsorptive filters may have a beneficial impact in those cases.
Software Aging in Container Orchestration Systems: An Assessment of Effects on Kubernetes
Authors: Tavares Jackson , Matos Rubens , Araujo Jean 
Published: August 2022

Abstract
Software Aging and Rejuvenation is a research topic that is being observed increasingly in the most diverse computing scenarios since the demand for reliability and availability of services has increased significantly. With the increasing use of containerized systems, there is also a need for the support of an orchestrator to ease management and reduce operational costs. In this paper, the behavior of Kubernetes software is evaluated, using both Minikube and K3S tools, while they manage pods in an accelerated lifetime experiment. Operations for creating and terminating pods are executed continuously, thus enabling us to observe the utilization of computing resources (e.g., CPU, memory, and I/O), the performance of the Minikube and K3S environments, and the response time of an application hosted in those environments. Software aging effects were observed in some conditions and for specific metrics, such as virtual memory usage, indicating a memory leak that is not fully cleared when the cluster is stopped. The issues found in this work may lead to system performance degradation and eventually compromise reliability and availability when the system crashes due to memory space exhaustion as well as full utilization of swap space on the hard disk.
Nonlinear Free Vibration of Functionally Graded Sandwich Beams Based on a Sinusoidal Shear Deformation Theory
Authors: Le Cong Ich , Nguyen Dinh Kien 
Published: August 2022

Abstract
Nonlinear free vibration of functionally graded sandwich beams is studied in this paper on the basis of a sinusoidal shear deformation theory and von Kármán geometric nonlinearity. The sandwich beams are composed of a pure ceramic core and two metal/ceramic functionally graded face sheets. The material properties of the face sheets are considered to be graded in the thickness by a power-law distribution, and they are evaluated by the Mori-Tanaka scheme. Nonlinear differential equations of motion are derived from Hamilton’s principle, and they are discretized by a two-node beam element. The direct iterative method is adopted to evaluate nonlinear frequencies of the sandwich beams with various boundary conditions. The numerical result reveals that the increase of the nonlinear frequency ratio by increasing the vibration amplitude is more significant for the beam associated with a higher power-law index. A parametric study is carried out to highlight the effect of the material and geometrical parameters on nonlinear vibration of the sandwich beams.
Comparison of Fluoride Uptake into Tooth Enamel Using Four Different Fluoride Varnishes
Authors: Moslemi Masumeh , Moghaddam Samira Asghari , Iranparvar Parastoo , Kooshki Fahimeh , Eskandarion Solmaz 
Published: August 2022

Abstract
Background
The efficacy of fluoride varnish in preventing dental caries has been well documented. The objective of this study was to compare the impact of four different fluoride varnish brands containing 5% sodium fluoride on the sound enamel of permanent teeth.
Methods
In this experimental study, 40 sound human premolar teeth were randomly assigned to four groups (Fluorilaq®, FluoroDose®, Preventa®, Kimia®; N = 10). Each tooth was sectioned into buccal and lingual halves, corresponding to the control and the experimental subgroups, respectively. In the experimental halves, a semi-circular shaped area of the enamel was treated with the designated fluoride varnish. All specimens underwent Acid Etch Enamel Biopsy. Fluoride and Calcium concentrations were measured using a Potentiometer and Inductively Coupled Plasma optical emission spectrometer, respectively. The collected data were analyzed using paired t-test and one-way ANOVA at P < 0.05.
Results
There was a significant difference between the enamel fluoride content of experimental and control halves in all groups (P < 0.05). Preventa® Fluoride varnish caused the highest Fluoride uptake, followed by FluoroDose®, Fluorilaq®, and Kimia®. All pair comparisons were statistically significant (P < 0.05), except for Preventa® vs. FluoroDose® (P = 0.36).
Conclusions
Since all examined Fluoride varnishes increased the Fluoride content of the tooth enamel, they potentially qualify for caries prevention. Furthermore, Preventa® could be considered a good domestic alternative for other brands investigated in this research, in terms of enamel fluoride uptake.
Volume-Outcome Relationship in Intra-abdominal Robotic-Assisted surgery. A Systematic Review
Authors: Day Elizabeth K , Galbraith Norman J , Ward Hester JT , Roxburgh Campbell S 
Published: August 2022

Abstract
As robotic assisted surgery (RAS) expands to smaller centres, platforms are shared between specialities. Healthcare providers must consider case volume and mix required to maintain quality and cost-effectiveness. This can be informed, in-part, by the volume-outcome relationship. We perform a systematic review to describe the volume-outcome relationship in intra-abdominal robotic assisted surgery to report on suggested minimum volumes standards. A literature search of Medline, NICE Evidence Search, Health Technology Assessment Database and Cochrane Library using the terms: “robot*”, “surgery”, “volume” and “outcome” was performed. The included procedures were gynaecological: hysterectomy, urological: partial and radical nephrectomy, cystectomy, prostatectomy, and general surgical: colectomy, oesophagectomy. Hospital and surgeon volume measures and all reported outcomes were analysed. 41 studies, including 983149 procedures, met the inclusion criteria. Study quality was assessed using the Newcastle-Ottawa Quality Assessment Scale and the retrieved data was synthesised in a narrative review. Significant volume-outcome relationships were described in relation to key outcome measures, including operative time, complications, positive margins, lymph node yield and cost. Annual surgeon and hospital volume thresholds were described. We concluded that in centres with an annual volume of fewer than 10 cases of a given procedure, having multiple surgeons performing these procedures led to worse outcomes and, therefore, opportunities should be sought to perform other complimentary robotic procedures or undertake joint cases.
Impact of type 1 diabetes and its duration on wall-to-lumen ratio and blood flow in retinal arterioles
Authors: Stefanski Adrian , Wolf Jacek , Harazny Joanna , Miszkowska-Nagórna Eliza , Wolnik Bogumił , Narkiewicz Krzysztof , Schmieder Roland 
Published: August 2022

Abstract
Background
Subclinical damage to both the small and large vessels may contribute to the development and progression of cardiovascular disease. Scanning laser Doppler flowmetry (SLDF), an established method used to measure retinal microcirculation, has been successfully applied in hypertensive and post-stroke patients.
Methods
Retinal microcirculation was assessed in 158 patients with type 1 diabetes and 38 age-matched healthy controls. The diabetics were divided into 3 groups: group A with diabetes duration < 12 months, group B with diabetes with 1–10 years, and group C > 10 years of diabetes. Retinal capillary structure and perfusion were evaluated using a Heidelberg retina flowmeter and automatically analyzed with full-field perfusion imaging.
Results
Age and BMI were comparable in all the diabetic patients and the controls (mean age 24.8 ± 4.7 years, mean BMI 22.9 ± 4.1). In the univariate analyses, RCF was significantly higher in group A (297 ± 121 arbitrary units [AU]) vs group B (236 ± 52 AU; p = 0.007) and group C (236 ± 70 AU; p = 0.008) and comparable to that of the controls (p = 0.46). Additionally, the WLR was highest in group C compared to the other diabetic subgroups and controls (p = 0.47).
Conclusions
New-onset diabetes is associated with an increase in RCF, which then gradually decreased with the duration of the disease. Structural changes of the retinal arterioles estimated via WLR are evident later in the course of diabetes, especially when the disease duration exceeded 10 years.
Diagnostic Approach for the Primary Cardiac Angiosarcoma – A Case Report
Authors: Shalji Shibaani , Bhatnagar Gopal , Hiremath Niranjan , Nahal Ayoub , Joshi Priti , Rivard Andrew 
Published: August 2022

Background:
We report a 25-year-old male who presented to an outside institution with progressive dyspnoea. He had increased jugular venous pressure and muffled heart sounds, suggesting a pericardial tamponade. A diagnosis of a cardiac angiosarcoma was put forward.
Primary cardiac tumours are rare, and patients can present with constitutional symptoms, posing a diagnostic challenge. The objective of this report is to suggest a diagnostic standard for early recognition of these fatal tumours.

Case presentation:
A chest radiograph demonstrated an enlarged cardiac contour and signs of pericardial tamponade. Pericardiocentesis was performed, complicated by a pneumopericardium and the pericardial fluid was haemorrhagic and exudative. Echocardiography confirmed a right atrial mass featured by heterogeneous enhancement in subsequent chest computed tomography and magnetic resonance imaging concerning a sarcoma. No metastatic lesions were found. The tumour was excised completely, and the following echocardiogram indicated normokinetic biventricular function. Histological examination of the tumour cells confirmed the presence of a cardiac angiosarcoma. Patient was scheduled for combined chemotherapy following discharge.

Conclusion:
Primary cardiac angiosarcomas are difficult to diagnose due to the rarity of the tumour as well as the non-specific symptoms. An early diagnosis may help improve prognosis which emphasizes the importance of establishing a standard diagnostic protocol for such tumours.
Sulfonamide Moiety as “Molecular Chimera” in the Design of New
Drugs
Authors: Culletta Giulia , Tutone Marco , Zappalà Maria , Almerico Anna Maria 
Published: August 2022

Background:
The -SO2NH- group is of great significance in modern pharmaceutical
use since, in sulfa-drugs, it is possible to introduce easily chemical modifications,
and even small changes may lead to an improved version of an already existing drug.

Objective:
This paper aims to describe updated information in the sulfonamide field with
a particular focus on new mechanisms of action, especially if discovered by employing
computational approaches.

Methods:
Research articles that focused on the use of the sulfonamide moiety for the design,
synthesis, and in vitro/in vivo tests of various diseases were collected from various
search engines like PubMed, Science Direct, Google Scholar, and Scopus, using keywords
like sulfonamide moiety, aryl/heteroary lsulfonamides, alkyl sulfonamides, in silico drug
design, etc.

Conclusion:
The more relevant reports highlighting the prominent role of sulfonamide
moiety in drug discovery have been critically analyzed. Sulfonamides can be considered
as “molecular chimera”, which are found to form hydrogen bonds as well as interact with
unipolar environments within proteins. Therefore, based on the analysis reported herein, it
is strongly foresight that new entities can be developed easily to improve the available
machinery helpful in the fight against new and emerging diseases.
Topoisomerase Enzyme Inhibitors as Potential Drugs Against Cancer: What Makes
Them Selective or Dual? – A Review
Authors: Gomes Joilly Nilce Santana , Santos Mirelly Barbosa , de Medeiros e Silva Yvnni Maria Sales , Albino Sonaly Lima , de Moura Ricardo Olímpio 
Published: August 2022

Abstract:
Topoisomerase inhibitors are extensively used in cancer chemotherapy. In the process of identifying novel anticancer compounds, biological evaluations are crucial and include, among others, the use of in silico and in vitro approaches. This work aimed to present recent research involving the obtainment and in silico and in vitro evaluation of topoisomerase I, II, and double inhibitors, of synthetic and natural origin, as potential compounds against tumor cells, in addition to proposing the construction of a desirable enzyme catalytic site. Therefore, it was observed that most Topoisomerase I inhibitors presented medium to large structures, with a rigid portion and a flexible region. In contrast, Topoisomerase IIα inhibitors showed medium and large structural characteristics, in addition to the planarity of the aromatic rings, which is mitigated due to flexible rings, but may also present elements that restrict conformation. The majority of compounds that exhibit dual inhibitory activity had relatively long chains, in addition to a flat and rigid portion suggestive of affinity for Topo I, and a flexible region characteristic of selective drugs for Topo II. Besides, it is noticed that most compounds that exhibit dual inhibitory showed similarities in the types of interactions and amino acids when compared to the selective compounds of Topo I and II. For instance, selective Topoisomerase I inhibitors interact with Arginine364 residues and selective Topoisomerase II inhibitors interact with Arginine487 residues, as both residues are targets for dual compounds.
Fasudil Protects Against Adriamycin-induced Acute Heart Injury by Inhibiting
Oxidative Stress, Apoptosis, and Cellular Senescence
Authors: Yan Yi , Xiang Chengyu , Zhang Dingguo 
Published: August 2022

Background:
The clinical utility of Adriamycin (ADR) is limited due to its toxicity, particularly cardiotoxicity.
Therefore, effective cardioprotective adjuvants to minimize ADR-induced acute cardiotoxicity are
urgently needed. Our previous studies have demonstrated the protective roles of fasudil on tissue injury. Here,
we further explore whether inhibition of Rho-kinase could alleviate the acute heart injury induced by ADR.

Methods:
C57BL6 mice were randomly divided into the following four groups: ① ADR group; ② low-dose
fasudil (ADR+L); ③ high-dose fasudil (ADR+H); and ④ control group (CON). Animals were injected i.p 20
mg/kg ADR once in group ①~③. Animals were injected i.p fasudil (2 or 10 mg/kg/day) daily for consecutive 6
days in groups ② and ③, respectively. Blood samples and heart tissues were collected for assays. H9C2 cells
were treated with fasudil for 30 mins and then incubated with ADR for 24 hours. Cells were collected for immunohistochemistry
and western blot study, respectively.

Results:
In the mouse model, administration of fasudil significantly ameliorated ADR-induced cardiac damage,
suppressed cell apoptosis and senescence, and ameliorated redox imbalance and DNA damage. In vitro, fasudil
treatment ameliorated ADR-induced immunofluorescence reaction of 8-OHdG, decreased the expression of
TUNEL cells and proteins of Bax, Caspase-3 and p53, and increased the expression of proteins of Bcl-2 and
SIRT 1.

Conclusion:
Fasudil has a protective effect on ADR induced acute cardiotoxicity, which is partially attributed
to its antioxidant, anti-senescence, and anti-apoptotic effects.
LncRNA NBR2 Regulates Cancer Cell Stemness and Predicts Survival in
Non-small Cell Cancer Patients by Downregulating TGF-β1
Authors: Huang Jun , Yu Qiuhua , Zhang Junjie , lu Wenbin , Liu Qian , Chu Ying , Zhou Yanjuan , Tu Renshu , Zhou Huxiang , Wang Qiang 
Published: August 2022

Background:
LncRNA NBR2 is a key regulator in cancer metabolism. However, its role in
lung cancer is unknown.

Objective:
This study aimed to explore the function of NBR2 in non-small cell lung cancer (NSCLC),
which is the most common type of lung cancer.

Methods:
Paired NSCLC and non-cancer tissues were collected from 68 patients with NSCLC. The
expression of NBR2 and transforming growth factor-β1 (TGF-β1) in these samples was analyzed by
RT-qPCR. The prognostic value of NBR2 for NSCLC was explored by performing a 5-year follow-up
study. The interaction between NBR2 and TGF-β1 in two NSCLC cell lines was detected by overexpression
assay, followed by RT-qPCR and Western blot analysis. Flow cytometry was performed to
evaluate the role of NBR2 and TGF-β1 in regulating NSCLC cell stemness.

Results:
NBR2 was significantly downregulated in NSCLC tissues than that in non-cancer tissues of
NSCLC patients, and low expression levels of NBR2 predicted poor survival. TGF-β1 was significantly
upregulated in NSCLC tissues than that in non-cancer tissues, and was inversely correlated with
NBR2. Overexpression of NBR2 downregulated TGF-β1, while overexpression of TGF-β1 did not
affect the expression of NBR2. Overexpression of NBR2 inhibited, while overexpression of TGF-β1
promoted NSCLC cell stemness. Overexpression of TGF-β1 attenuated the effects of overexpression
of NBR2. Mechanically, NBR2 interacted with Notch1 protein to inhibit its expression, thereby inhibiting
the expression of TGF-β1 and further affecting the proportion of CD133+ cells.

Conclusion:
LncRNA NBR2 regulates cancer cell stemness and predicts survival in NSCLC possibly
by downregulating TGF-β1 through Notch1.
Impurities Present in Cardiovascular Active Substances and Medicinal
Products: A Pharmacopoeial Perspective
Authors: Sharma Arvind K. , Ali Faraat , Prakash Anuj , Goyal Ramesh K. 
Published: August 2022

Abstract:
The quality of drugs is a major concern for drug regulatory authorities and other stake-holders across the globe. Recently, drug regulatory authorities across the globe are facing a chal-lenge in controlling the purity of cardiovascular (CVS) drugs for human use, especially drugs from the Angiotensin Receptor Blocker family, such as Valsartan. The present article aims to provide comprehensive knowledge on how pharmacopeias worldwide play a key role in ensuring the quality of active pharmaceutical ingredients (API) and finished pharmaceutical products (FPPs). In this ar-ticle, the focus is on comprehensive information regarding pharmaceutical impurities, separation strategies, relevant regulatory guidelines to control impurities, and their acceptable limits, particu-larly with respect to cardiovascular active drug substances and drug formulations for human use.
Current Outlooks on Machine Learning Methods for the Development of
Industrial Homogeneous Catalytic Systems
Authors: Ferraz-Caetano José 
Published: August 2022

Abstract:
This brief perspective outlines the pivotal role of Machine Learning methods in the green, digital transition of industrial chemistry. The focus on homogenous catalysis highlights the recent methodologies in the development of industrial processes, including the design of new catalysts and the enhancement of sustainable reaction conditions to lower production costs. We report several examples of Machine Learning assisted methodologies through recent Data Science trends on innovation of industrial homogeneous organocatalytic systems. We also stress the current benefits, drawbacks, and limitations towards the mass implementation of these Data Science methodologies.