bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tecnologia educacional: Orientação no autocuidado à pacientes idosos da alta da clínica médica para o domicílio
Authors: Santos José Daniel Rodrigues dos , Santos Valeria Regina Cavalcante dos , Mendonça Xaene Maria Fernandes Duarte , Ferreira Ilma Pastana , Albuquerque Jacirene Vasconcelos de , Siqueira Bianca Camila Neves , Coelho Vitória Fernanda Valadares , Gonçalves Brenda Laryssa Lima , Souza Cristina Frassinette Lima , Trindade Creusa Barbosa dos Santos 
Published: August 2022

Objetivo: Construir uma cartilha educacional para orientação de autocuidado a pacientes idosos na alta da clínica médica para o domicílio Método: Trata de pesquisa-ação com abordagem quanti-qualitativa desenvolvida na FSCMP no setor de clínica médica. A população do estudo foram 84 pacientes idosos com déficit no autocuidado, em processo de alta para o domicílio. Para coleta de dados foi aplicado um instrumento semiestruturado, compondo dados sociodemográficos, econômicos, clínicos; internação, capacidade para o autocuidado, percepções sobre alta hospitalar e dificuldades no autocuidado. As entrevistas foram processadas segundo a análise de conteúdo do método de Bardin. As variáveis quantitativas foram distribuídas em frequências absolutas e relativas. Resultados: A amostra predominou idosos do sexo masculino (66,67%), faixa etária de 65 a 69 anos (42,85%), renda familiar de até 01 salário mínimo (53,57%), união estável (33,33%), grau de escolaridade do ensino fundamental (61,91%), pardos (78,54%) e aposentados (41,67%). A maioria possui tempo prolongado de internação sendo 33,34% superiores a 30 dias, 77,38% são polifarmácias, 46,43% apresentam dependência importante. O diagnóstico mais evidenciado foi doença do sistema digestivo (42,85%). Quanto a compreensão da hospitalização e alta hospitalar, os significados que os sujeitos atribuíram foram classificados em três categorias temáticas: A-A importância do apoio familiar/cuidadores; B–A manutenção financeira da família; C–Cuidados gerais referidos de autocuidado. Conclusão: Há necessidade de estudos e construção de mais tipos de produtos com tecnologias educativas que busque abranger a maior quantidade possível de orientações de autocuidado a pacientes idosos pós-alta hospitalar.
Um olhar renovado sobre o chamado divino a ‘cuidar e cultivar’: a questão da essência administrativa
Authors: Lovo Odirlei Arcangelo , Sanches Mário Antônio 
Published: August 2022

O objetivo deste artigo é propor uma reflexão sobre o próprio ser humano, que é chamado a colaborar com Deus no cuidar e cultivar a criação. Apresenta-se a indagação: o que possibilita à pessoa ser um colaborador de Deus? Trata-se de construção de narrativa, para qual os dois núcleos fundamentais de dados, textos e informações foram a Doutrina Social da Igreja e os documentos do Magistério que elucidam o projeto, a vivência e a dinâmica familiar. A narrativa apresenta que a pessoa, em esponsalidade com o Criador, se torna o ato administrativo de Deus no mundo, elucida-se, então, a ‘essência administrativa’, o dom primeiro, com o qual Deus dirige-se ao ser humano no chamado/mandado a cuidar e cultivar o jardim. Suscita-se a pessoa enquanto o ato administrativo de Deus no mundo, fomentando que é em família que os filhos/Ben de Deus devem cuidar da terra e se tronar a nação dos filhos/povo de Deus, nação sem limitações geográficas, alicerçada no amor e na confiança no Reino de Deus que se faz na contingência da criação sob a administração humana.
Tratamento da hipertensão arterial sistêmica: uma revisão de literatura integrativa sobre Hidroclorotiazida vs Clortalidona
Authors: Nascimento Yan Santiago , Nascimento Yago Santiago , Ferreira Letícia Maria Rendall , Cunha Neto Cícero Pereira , Santos Caio Roberto Alexandre Cavalcante dos , Menezes Arthur Souza , Santos Junior Ricardo Ferreira dos 
Published: August 2022

Objetivo: Realizar uma revisão de literatura integrativa buscando analisar as principais diferenças no uso da Hidroclorotiazida vs Clortalidona, afim de entender se algum medicamento se sobrepõe sobre o outro. Metodologia: Foi realizada uma revisão de literatura integrativa, analisando-se artigos desde janeiro de 2010 até junho de 2022, nos idiomas português, inglês e espanhol nas seguintes bases de dados: MEDLINE via PubMed, Europe PMC, Lilacs e SciELO. Os descritores utilizados foram “Clortalidona”, “Hidroclorotiazida” e “Hipertensão”, de acordo com o DeCS, abrangendo ao final 31 artigos. Resultados: Diversos estudos apontam para o efeito mais intenso da clortalidona sobre a hidroclorotiazida, mas ressaltam que o primeiro possui maiores efeitos adversos, como hipocalemia e hiponatremia. Algumas pesquisas trazem que o uso da clortalidona sobre a hidroclorotiazida trouxe uma redução de risco cardiovascular de aproximadamente 18%, sendo um resultado bastante debatido na literatura por ser fruto de meta-análise indireta. Conclusão: A literatura ainda diverge quanto ao uso da clortalidona sobre a hidroclorotiazida, necessitando de mais estudos conclusivos sobre o efeito direto comparativo entre as duas drogas. Os dados da literatura convergem que a clortalidona tem um efeito mais intenso que a hidroclorotiazida, trazendo, no entanto, mais efeitos adversos para o paciente, como hipercalemia, por exemplo.
Novel miniaturized fluorescence loop-mediated isothermal amplification detection system for rapid on-site virus detection
Authors: Wu Yanqi , Bai Liping , Ye Chengfu , Yuhong Guan  , Kunming Yan  , Chen Hui , Jiang Zhihong 
Published: August 2022

New pathogen outbreaks have progressed rapidly and are highly infectious in recent years, increasing the urgency of rapid and accurate detection of pathogenic microorganisms. Based on the point-of-care testing (POCT) requirements, in this study, a real-time fluorescent loop-mediated isothermal amplification (LAMP) detection system was developed and applied to pathogen detection. The system is compact and portable, with good uniformity and reproducibility, and it can detect pathogens rapidly and effectively. For norovirus detection, the linear range was 100–106 copies/μL. The system can achieve the theoretical sensitivity of LAMP detection, conclusions could be obtained within 35 min, and quantitative detection was possible. The test results of 45 clinical samples were consistent with quantitative PCR (qPCR) and clinical results, and the accuracy could reach 100%. This system has the characteristics of portability, speed, and POCT accuracy, and the cost is much lower than that of commercial qPCR. Therefore, it is suitable for remote areas or places with relatively poor conditions and environments requiring on-site conditions. It can also be widely used to detect various epidemics and unexpected diseases.
Radiation therapy affects YAP expression and intracellular localization by modulating lamin A/C levels in breast cancer
Authors: La Verde Giuseppe , Artiola Valeria , Pugliese Mariagabriella , La Commara Marco , Arrichiello Cecilia , Muto Paolo , Netti Paolo A. , Fusco Sabato , Panzetta Valeria 
Published: August 2022

The microenvironment of breast cancer actively participates in tumorigenesis and cancer progression. The changes observed in the architecture of the extracellular matrix initiate an oncogene-mediated cell reprogramming, that leads to a massive triggering of YAP nuclear entry, and, therefore, to cancer cell proliferation, invasion and probably to increased radiation-resistance. However, it is not yet fully understood how radiotherapy regulates the expression and subcellular localization of YAP in breast cancer cells experiencing different microenvironmental stiffnesses. To elucidate the role of extracellular matrix stiffness and ionizing radiations on YAP regulation, we explored the behaviour of two different mammary cell lines, a normal epithelial cell line (MCF10A) and a highly aggressive and invasive adenocarcinoma cell line (MDA-MB-231) interacting with polyacrylamide substrates mimicking the mechanics of both normal and tumour tissues (∼1 and ∼13 kPa). We report that X-ray radiation affected in a significant way the levels of YAP expression, density, and localization in both cell lines. After 24 h, MCF10A and MDA-MB-231 increased the expression level of YAP in both nucleus and cytoplasm in a dose dependent manner and particularly on the stiffer substrates. After 72 h, MCF10A reduced mostly the YAP expression in the cytoplasm, whereas it remained high in the nucleus of cells on stiffer substrates. Tumour cells continued to exhibit higher levels of YAP expression, especially in the cytoplasmic compartment, as indicated by the reduction of nuclear/cytoplasmic ratio of total YAP. Then, we investigated the existence of a correlation between YAP localization and the expression of the nuclear envelope protein lamin A/C, considering its key role in modulating nuclear deformability and changes in YAP shuttling phenomena. As supposed, we found that the effects of radiation on YAP nucleus/cytoplasmic expression ratio, increasing in healthy cells and decreasing in tumour ones, were accompanied by lower and higher lamin A/C levels in MCF10A and MDA-MB-231 cells, respectively. These findings point to obtain a deeper knowledge of the role of the extracellular matrix and the effects of X-rays on YAP and lamin A/C expression that can be used in the design of doses and timing of radiation therapy.
A novel electrochemical immunosensor based on PdAgPt/MoS2 for the ultrasensitive detection of CA 242
Authors: Cao Linlin , Lu Sumei , Guo Chengjie , Chen Wenqiang , Gao Yinan , Ye Diwen , Guo Zejun , Ma Wanshan 
Published: August 2022

Dynamic monitoring of tumor markers is an important way to the diagnosis of malignant tumor, evaluate the therapeutic effect of tumor and analyze the prognosis of cancer patients. As a tumor marker of digestive tract, CA242 is often used to Assess the therapeutic effect of colorectal cancer and pancreatic cancer. In this study, immunosensor technology was used to detect CA242. PdAgPt nanocomposites, which have great advantages in biocompatibility, electrical conductivity and catalytic properties, were prepared by hydrothermal synthesis method. The prepared PdAgPt nanocomposites were loaded onto the surface of molybdenum disulfide (MoS2) with large surface area, and the new nanocomposites were synthesized. Using PdAgPt/MoS2 as signal amplification platform, the label-free CA242 electrochemical immunosensor has a wide detection range that extends from 1*10−4 U/ml to 1*102 U/ml and a low detection limit (LOD, 3.43*10−5 U/ml) after optimization of experimental conditions. In addition, the CA242 immunosensor designed in this study also performed well in the evaluation of repeatability, selectivity and stability, and was successfully used for the detection of CA242 in human serum sample. Therefore, the label-free electrochemical immunosensor constructed in this study has a broad application prospect in the detection of clinical biomarkers.
Ligand-bound integrin αvβ6 internalisation and trafficking
Authors: Meecham Amelia , Cutmore Lauren C. , Protopapa Pantelitsa , Rigby Lauren G. , Marshall John F. 
Published: August 2022

The integrin αvβ6 is expressed at low levels in most normal healthy tissue but is very often upregulated in a disease context including cancer and fibrosis. Integrins use endocytosis and trafficking as a means of regulating their surface expression and thus their functions, however little is known of how this process is regulated in the context of αvβ6. As αvβ6 is a major target for the development of therapeutics in cancer and fibrosis, understanding these dynamics is critical in the development of αvβ6-targeted therapies. Following development of a flow cytometry-based assay to measure ligand (A20FMDV2 or LAP)-bound αvβ6 endocytosis, an siRNA screen was performed to identify which genes were responsible for internalising αvβ6. These data identified 15 genes (DNM2, CBLB, DNM3, CBL, EEA1, CLTC, ARFGAP3, CAV1, CYTH2, CAV3, CAV2, IQSEC1, AP2M1, TSG101) which significantly decreased endocytosis, predominantly within dynamin-dependent pathways. Inhibition of these dynamin-dependent pathways significantly reduced αvβ6-dependent migration (αvβ6-specific migration was 547 ± 128 under control conditions, reduced to 225 ± 73 with clathrin inhibition, and 280 ± 51 with caveolin inhibition). Colocalization studies of αvβ6 with endosome markers revealed that up to 6 h post-internalisation of ligand, αvβ6 remains in Rab11-positive endosomes in a perinuclear location, with no evidence of αvβ6 degradation up to 48 h post exposure to A20FMDV2. Additionally, 60% of ligand-bound αvβ6 was recycled back to the surface by 6 h. With studies ongoing using conjugated A20FMDV2 to therapeutically target αvβ6 in cancer and fibrosis, these data have important implications. Binding of A20FMDV2 seemingly removes much of the αvβ6 from the cell membrane, and upon its recycling, a large fraction appears to still be in the ligand-bound state. While these results are observed with A20FMDV2, these data will be of value in the design of αvβ6-specific therapeutics and potentially the types of therapeutic load.
Design of highly active Ni catalysts supported on carbon nanofibers for the hydrolytic hydrogenation of cellobiose
Authors: Frecha Esther , Remón Javier , Torres Daniel , Suelves Isabel , Pinilla José Luis 
Published: August 2022

The direct transformation of cellulose into sugar alcohols (one-pot conversion) over supported nickel catalysts represents an attractive chemical route for biomass valorization, allowing the use of subcritical water in the hydrolysis step. The effectiveness of this process is substantially conditioned by the hydrogenation ability of the catalyst, determined by design parameters such as the active phase loading and particle size. Herein, mechanistic insights into catalyst design to produce superior activity were outlined using the hydrolytic hydrogenation of cellobiose as a model reaction. Variations in the impregnation technique (precipitation in basic media, incipient wetness impregnation, and the use of colloidal-deposition approaches) endowed carbon-nanofiber-supported catalysts within a wide range of Ni crystal sizes (5.8–20.4 nm) and loadings (5–14 wt%). The link between the properties of these catalysts and their reactivity has been established using characterization techniques such as X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy, and inductively coupled plasma-optical emission spectroscopy (ICP-OES). A fair compromise was found between the Ni surface area (3.89 m2/g) and its resistance against oxidation for intermediate crystallite sizes (∼11.3 nm) loaded at 10.7 wt%, affording the hydrogenation of 81.2% cellobiose to sorbitol after 3 h reaction at 190°C and 4.0 MPa H2 (measured at room temperature). The facile oxidation of smaller Ni particle sizes impeded the use of highly dispersed catalysts to reduce the metal content requirements.
Menopause-related cortical loss of the humeral head region mainly occurred in the greater tuberosity
Authors: Wang Yeming , Li Jian , Men Yutao , Wei Wanfu 
Published: August 2022

AimsProximal humerus fractures are commonly observed in postmenopausal women. The goal of this study was to investigate menopause-related changes in cortical structure of the humeral head.Materials and methodsClinical computed tomography (CT) scans of 75 healthy women spanning a wide range of ages (20–72 years) were analyzed. For each subject, cortical bone mapping (CBM) was applied to create a color three-dimensional (3D) thickness map for the proximal humerus. Nine regions of interest (ROIs) were defined in three walls of the humeral head. Cortical parameters, including the cortical thickness (CTh), cortical mass surface density (CM), and the endocortical trabecular density (ECTD), were measured.ResultsCompared to premenopausal women, postmenopausal women were characterized by a significantly lower CTh and CM value in the lateral part of the greater tuberosity. Similar changes were only found in ROI 4, but not in ROIs 5–6 in the lesser tuberosity. Linear regression analysis revealed that the CTh and CM value of ROIs 1, 3, and 4 were negatively associated with age. These results showed that menopause-related loss in CTh and CM was mainly in the greater tuberosity besides the proximal part of the lesser tuberosity. Trabecular bone variable measured as ECTD showed a notably lower value in ROIs 1–9 in postmenopausal vs. premenopausal group. Inverse linear associations for ECTD and age were found in ROIs 2, 3, 5, 6, 7, and 9, indicating no site-specific differences of endocortical trabecular bone loss between the greater and lesser tuberosity.ConclusionsMenopause-related cortical loss of the humeral head mainly occurred in the lateral part of the greater tuberosity. The increased rate of humeral bone loss in the greater tuberosity may contribute materially to complex proximal humerus fractures.
XBP1 impacts lung adenocarcinoma progression by promoting plasma cell adaptation to the tumor microenvironment
Authors: Zhong Zhaoqian , Wang Junhao , Han Qizheng , Lin Hong , Luo Haihua , Guo Danyan , Jiang Yong , Liu Aihua 
Published: August 2022

Background: The activation of X-box binding protein 1 (XBP1) plays an essential role in the unfolded protein response (UPR) of the endoplasmic reticulum (ER). XBP1 is commonly expressed in various tumors and is closely related to tumorigenesis and progression. However, the role of XBP1 in lung adenocarcinoma (LUAD), especially the prognostic value of its alternative splicing isoforms, remains largely unknown.Methods: The LUAD datasets were retrieved from the The Cancer Genome Atlas, ArrayExpress and Gene Expression Omnibus. GEPIA2 and meta-analysis were employed to explore the prognostic value, and bioinformatics analysis with the TIMER2.0 database was used to investigate immune cell infiltration. We performed single-cell analyses to identify cell types with high XBP1 expression. In addition, polymerase chain reaction (PCR) and DNA sequencing were performed to verify the authenticity of the new spliceosome.Results: In this study, we found that high expression of XBP1 was significantly associated with a good prognosis, and XBP1 expression was significantly positively correlated with B cell infiltration in LUAD. In addition, we found that high-level expression of a novel splicing isoform, XBP1 (XBP1-003), improved the prognosis of LUAD. Protein structural analysis demonstrated that XBP1-003 has several specific protein domains that are different from those of other XBP1 isoforms, indicating a unique function of this isoform in LUAD.Conclusion: All these results suggest that XBP1 plays an antitumorigenic role in LUAD through alternative splicing, which may be related to the adaptation of plasma cells. This sheds new light on the potential strategy for LUAD prognosis evaluation and immunotherapy.
Global research trends on the links between gut microbiota and cancer immunotherapy: A bibliometric analysis (2012-2021)
Authors: Yang Shanshan , Zhao Suya , Ye Yixiang , Jia Liqun , Lou Yanni 
Published: August 2022

BackgroundThere is a crosstalk between gut microbiota (GM) and cancer immunotherapy (CI). The purpose of this study is to use bibliometric analysis to identify the highly cited papers relating to GM/CI and explore the research status and development trends of the GM/CI research.MethodsA literature search regarding GM/CI publications from 2012 to 2021 was undertaken on July 4, 2022. The article titles, journals, authors, institutions, countries, total citations, keywords, and other information were extracted from the Science Citation Index Expanded (SCIE) of Web of Science Core Collection (WoSCC). The Bibliometrix of R package and VOSviewer were used for bibliometric analysis.ResultsA total of 665 papers were extracted. The number of papers has increased rapidly over the past decade, especially after 2018. The United States and China had the most publications and made great contributions to this field. Th5e Univ Texas MD Anderson Canc Ctr and Univ Paris Saclay were absolutely in the leading position in GM/CI. The most influential authors were Zitvogel L and Routy B. Frontiers in Immunology had the most publications and Science had the most total citations. Historical direct citation analysis explained the historical evolution in GM/CI. Highly cited papers and high-frequency keywords illustrated the current status and trends of GM/CI. Four clusters were identified and the important topics included the role of GM and antibiotics in CI, the methods of targeting GM to improve CI outcomes, the mechanism by which GM affects CI and the application of ICIs in melanoma. “Tumor microbiome”, “proton pump inhibitors” and “prognosis” may be the new focus of attention in the next few years.ConclusionThis study filtered global publications on GM/CI correlation and analyzed their bibliometric characteristics, identified the most cited papers in GM/CI, and gained insight into the status, hotspots and trends of global GM/CI research, which may inform researchers and practitioners of future directions.
Stevens-Johnson syndrome and toxic epidermal necrolysis: A systematic review of PubMed/MEDLINE case reports from 1980 to 2020
Authors: Wang Liqin , Varghese Sheril , Bassir Fatima , Lo Ying-Chin , Ortega Carlos A. , Shah Sonam , Blumenthal Kimberly G. , Phillips Elizabeth J. , Zhou Li 
Published: August 2022

BackgroundStevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are rare, life-threatening immunologic reactions. Prior studies using electronic health records, registries or reporting databases are often limited in sample size or lack clinical details. We reviewed diverse detailed case reports published over four decades.MethodsStevens-Johnson syndrome and toxic epidermal necrolysis-related case reports were identified from the MEDLINE database between 1980 and 2020. Each report was classified by severity (i.e., SJS, TEN, or SJS-TEN overlap) after being considered a “probable” or “definite” SJS/TEN case. The demographics, preconditions, culprit agents, clinical course, and mortality of the cases were analyzed across the disease severity.ResultsAmong 1,059 “probable” or “definite” cases, there were 381 (36.0%) SJS, 602 (56.8%) TEN, and 76 (7.2%) SJS-TEN overlap cases, with a mortality rate of 6.3%, 24.4%, and 21.1%, respectively. Over one-third of cases had immunocompromised conditions preceding onset, including cancer (n = 194,18.3%), autoimmune diseases (n = 97, 9.2%), and human immunodeficiency virus (HIV) (n = 52, 4.9%). During the acute phase of the reaction, 843 (79.5%) cases reported mucous membrane involvement and 210 (19.8%) involved visceral organs. Most cases were drug-induced (n = 957, 90.3%). A total of 379 drug culprits were reported; the most frequently reported drug were antibiotics (n = 285, 26.9%), followed by anticonvulsants (n = 196, 18.5%), analgesics/anesthetics (n = 126, 11.9%), and antineoplastics (n = 120, 11.3%). 127 (12.0%) cases reported non-drug culprits, including infections (n = 68, 6.4%), of which 44 were associated with a mycoplasma pneumoniae infection and radiotherapy (n = 27, 2.5%).ConclusionAn expansive list of potential causative agents were identified from a large set of literature-reported SJS/TEN cases, which warrant future investigation to understand risk factors and clinical manifestations of SJS/TEN in different populations.