bluuebunny/crossref_metadata_2025_split huggingface dataset:
CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I
Authors: Zhou Cheng-Jie , Wang Xing-Yue , Dong Yan-Hua , Wang Dong-Hui , Han Zhe , Zhang Xiao-Jie , Sun Qing-Yuan , Carroll John , Liang Cheng-Guang 
Published: December 2022

AbstractChromosome segregation is initiated by cohesin degradation, which is driven by anaphase-promoting complex/cyclosome (APC/C). Chromosome cohesin is removed by activated separase, with the degradation of securin and cyclinB1. Dynamin-related protein 1 (DRP1), a component of the mitochondrial fission machinery, is related to cyclin dynamics in mitosis progression. Here, we show that DRP1 is recruited to the kinetochore by centromeric Centromere protein F (CENP-F) after nuclear envelope breakdown in mouse oocytes. Loss of DRP1 during prometaphase leads to premature cohesin degradation and chromosome segregation. Importantly, acute DRP1 depletion activates separase by initiating cyclinB1 and securin degradation during the metaphase-to-anaphase transition. Finally, we demonstrate that DRP1 is bound to APC2 to restrain the E3 ligase activity of APC/C. In conclusion, DRP1 is a CENP-F-dependent atypical spindle assembly checkpoint (SAC) protein that modulates metaphase-to-anaphase transition by controlling APC/C activity during meiosis I in oocytes.
Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass
Authors: Sun Guangchao , Wase Nishikant , Shu Shengqiang , Jenkins Jerry , Zhou Bangjun , Torres-Rodríguez J. Vladimir , Chen Cindy , Sandor Laura , Plott Chris , Yoshinga Yuko , Daum Christopher , Qi Peng , Barry Kerrie , Lipzen Anna , Berry Luke , Pedersen Connor , Gottilla Thomas , Foltz Ashley , Yu Huihui , O’Malley Ronan , Zhang Chi , Devos Katrien M. , Sigmon Brandi , Yu Bin , Obata Toshihiro , Schmutz Jeremy , Schnable James C. 
Published: December 2022

AbstractA number of crop wild relatives can tolerate extreme stress to a degree outside the range observed in their domesticated relatives. However, it is unclear whether or how the molecular mechanisms employed by these species can be translated to domesticated crops. Paspalum (Paspalum vaginatum) is a self-incompatible and multiply stress-tolerant wild relative of maize and sorghum. Here, we describe the sequencing and pseudomolecule level assembly of a vegetatively propagated accession of P. vaginatum. Phylogenetic analysis based on 6,151 single-copy syntenic orthologues conserved in 6 related grass species places paspalum as an outgroup of the maize-sorghum clade. In parallel metabolic experiments, paspalum, but neither maize nor sorghum, exhibits a significant increase in trehalose when grown under nutrient-deficit conditions. Inducing trehalose accumulation in maize, imitating the metabolic phenotype of paspalum, results in autophagy dependent increases in biomass accumulation.
Genetic approaches for assessment of phosphorus use efficiency in groundnut (Arachis hypogaea L.)
Authors: Kadirimangalam Sai Rekha , Jadhav Yashoda , Nagamadhuri K. V. , Putta Latha , Murugesan Tharanya , Variath Murali T. , Vemula Anil Kumar , Manohar Surendra Singh , Chaudhari Sunil , Choudhary Sunita , Kholova Jana , Pasupuleti Janila 
Published: December 2022

AbstractProduction of phosphorus efficient genotypes can reduce environmental pollution. Identification of P-efficient groundnut genotypes is a need of the hour to sustain in P-deficient soils. The pot experiment showed significant differences between genotypes (G) and treatments (T) for all the traits and G × T interaction for majority of traits. The G × T × Y interaction effects were also significant for all the traits except leaf P% (LP%), leaf acid phosphatase (LAP) and root dry weight (RDW). In lysimeter experiment, the effect of G, T and G × T were significant for leaf dry weight (LDW), stem dry weight (SDW), total transpiration (TT) and transpiration efficiency (TE). For traits, LDW, SDW, TT, TE, ICGV 00351 and ICGS 76; for SDW, TT, ICGV 02266 are best performers under both P-sufficient and deficient conditions. Based on P-efficiency indices and surrogate traits of P-uptake, ICGV’s 02266, 05155, 00308, 06040 and 06146 were considered as efficient P-responding genotypes. From GGE biplot, ICGV 06146 under P-deficient and TAG 24 under both P-sufficient and deficient conditions are portrayed as best performer. ICGV 06146 was identified as stable pod yielder and a promising genotype for P-deficient soils. The genotypes identified in this study can be used as a parent in developing mapping population to decipher the genetics and to devleop groundnut breeding lines suitable to P-deficient soils.
Metformin enhances the antitumor activity of oncolytic herpes simplex virus HF10 (canerpaturev) in a pancreatic cell cancer subcutaneous model
Authors: Abdelmoneim Mohamed , Eissa Ibrahim Ragab , Aboalela Mona Alhussein , Naoe Yoshinori , Matsumura Shigeru , Sibal Patricia Angela , Bustos-Villalobos Itzel , Tanaka Maki , Kodera Yasuhiro , Kasuya Hideki 
Published: December 2022

AbstractOncolytic virus (OV) therapy is a promising cancer immunotherapy, especially for cold tumors by inducing the direct lysis of cancer cells and initiation of potent antitumor response. Canerpaturev (C-REV) is an attenuated oncolytic herpes simplex virus-1, which demonstrated a potent antitumor effect in various preclinical models when used either alone or combined. Metformin is a commonly prescribed antidiabetic drug that demonstrated a potent immune modulator effect and antitumor response. We combined C-REV with metformin in a low immunogenic bilateral murine tumor model to enhance C-REV’s antitumor efficacy. In vitro, metformin does not enhance the C-REV cell cytotoxic effect. However, in in vivo model, intratumoral administration of C-REV with the systemic administration of metformin led to synergistic antitumor effect on both sides of tumor and prolonged survival. Moreover, combination therapy increased the effector CD44+ CD8+ PD1- subset and decreased the proportion of terminally-differentiated CD103+ KLRG-1+ T-regulatory cells on both sides of tumor. Interestingly, combination therapy efficiently modulates conventional dendritic cells type-1 (cDC1) on tumors, and tumor-drained lymph nodes. Our findings suggest that combination of C-REV and metformin enhances systemic antitumor immunity. This study may provide insights into the mechanism of action of OV therapy plus metformin combination against various tumor models.
Mendelian randomization study of the genetic interaction between psoriasis and celiac disease
Authors: Li Lin , Fu Lixin , Zhang Liwen , Feng Yanyan 
Published: December 2022

AbstractEpidemiological studies have observed some relationship between psoriasis and celiac disease (CD), while the causal link between these 2 autoimmune diseases was unclear. In the current study, we aimed to explore the causal link between psoriasis and celiac disease with bidirectional 2-sample Mendelian Randomization (MR) study. Eligible instrument variables (IVs) with genome-wide significance (p < 5E−08) were extracted from the summary-level datasets from the published genome-wide association studies (GWAS), which were conducted in the European population. The inverse variance weighted (IVW) method was performed as the main analysis, sensitivity analyses and post-MR analyses were also performed. Our MR analyses found that genetically doubling the odds of CD would increase the risk for psoriasis (p = 1.58e−03, OR [95% CI] 1.232 [1.061–1.432]). And the results were supported by sensitivity analyses. While we found that genetically determined psoriasis was not associated with the risk for CD (IVW: p = 0.985, OR [95% CI] 1.000 [0.965–1.037]). Our study provided novel genetic evidence that patients with CD were at an increased risk of developing psoriasis, while psoriasis was not associated with the risk for CD. Clinicians should be aware of the associations and pay attention to skin manifestations in patients with CD.
Pan-cancer functional analysis of somatic mutations in G protein-coupled receptors
Authors: Bongers B. J. , Gorostiola González M. , Wang X. , van Vlijmen H. W. T. , Jespers W. , Gutiérrez-de-Terán H. , Ye K. , IJzerman A. P. , Heitman L. H. , van Westen G. J. P. 
Published: December 2022

AbstractG Protein-coupled receptors (GPCRs) are the most frequently exploited drug target family, moreover they are often found mutated in cancer. Here we used a dataset of mutations found in patient samples derived from the Genomic Data Commons and compared it to the natural human variance as exemplified by data from the 1000 genomes project. We explored cancer-related mutation patterns in all GPCR classes combined and individually. While the location of the mutations across the protein domains did not differ significantly in the two datasets, a mutation enrichment in cancer patients was observed among class-specific conserved motifs in GPCRs such as the Class A “DRY” motif. A Two-Entropy Analysis confirmed the correlation between residue conservation and cancer-related mutation frequency. We subsequently created a ranking of high scoring GPCRs, using a multi-objective approach (Pareto Front Ranking). Our approach was confirmed by re-discovery of established cancer targets such as the LPA and mGlu receptor families, but also discovered novel GPCRs which had not been linked to cancer before such as the P2Y Receptor 10 (P2RY10). Overall, this study presents a list of GPCRs that are amenable to experimental follow up to elucidate their role in cancer.
Mesenchymal stromal cells donate mitochondria to articular chondrocytes exposed to mitochondrial, environmental, and mechanical stress
Authors: Fahey Megan , Bennett Maureen , Thomas Matthew , Montney Kaylee , Vivancos-Koopman Irene , Pugliese Brenna , Browning Lindsay , Bonassar Lawrence J. , Delco Michelle 
Published: December 2022

AbstractArticular cartilage has limited healing capacity and no drugs are available that can prevent or slow the development of osteoarthritis (OA) after joint injury. Mesenchymal stromal cell (MSC)-based regenerative therapies for OA are increasingly common, but questions regarding their mechanisms of action remain. Our group recently reported that although cartilage is avascular and relatively metabolically quiescent, injury induces chondrocyte mitochondrial dysfunction, driving cartilage degradation and OA. MSCs are known to rescue injured cells and improve healing by donating healthy mitochondria in highly metabolic tissues, but mitochondrial transfer has not been investigated in cartilage. Here, we demonstrate that MSCs transfer mitochondria to stressed chondrocytes in cell culture and in injured cartilage tissue. Conditions known to induce chondrocyte mitochondrial dysfunction, including stimulation with rotenone/antimycin and hyperoxia, increased transfer. MSC-chondrocyte mitochondrial transfer was blocked by non-specific and specific (connexin-43) gap-junction inhibition. When exposed to mechanically injured cartilage, MSCs localized to areas of matrix damage and extended cellular processes deep into microcracks, delivering mitochondria to chondrocytes. This work provides insights into the chemical, environmental, and mechanical conditions that can elicit MSC-chondrocyte mitochondrial transfer in vitro and in situ, and our findings suggest a new potential role for MSC-based therapeutics after cartilage injury.
Calcium as an innovative and effective catalyst for the synthesis of graphene-like materials from cellulose
Authors: Béguerie Théotime , Weiss-Hortala Elsa , Nzihou Ange 
Published: December 2022

AbstractPyrolysis of lignocellulosic biomass (hard carbon) produces poorly graphitic biochar. In this study, nano-structured biochars were produced from microcrystalline cellulose using calcium as a non-conventional catalyst. Calcium is abundant, environmental-friendly and widely accessible. Graphitization of calcium-impregnated cellulose was carried out at 1800 °C, a temperature below 2000 °C where the graphitization usually occurs. XRD, Raman spectroscopy, high-resolution TEM together with the in-house numerical tool developed enable the quantification of the graphene fringes in the biochars. The non-impregnated cellulose biochar was composed of short and poorly stacked graphene fringes. The impregnation with 2 wt.% of calcium led to the conversion of the initial structure into a well-organized and less defective graphene-like one. The graphene-like structures obtained were composed of tens of stacked graphene fringes with a crystallite size up to 20 nm and an average interlayer spacing equal to 0.345 nm, close to the reference value of standard hexagonal graphite (0.3354 nm). The increase of the calcium concentration did not significantly improve the crystallite sizes of the graphene-like materials but rather drastically improved their rate. Our results propose a mechanism and provide new insights on the synthesis of graphene-like materials from bio-feedstocks using calcium where the literature is focused on transition metals such as iron and nickel among others. The decrease of the graphitization temperature below 2000 °C should lower the production cost as well as the environmental impact of the thermal graphene-like materials synthesis using biomass. This finding should stimulate further research in the field and broaden the application perspectives.
Root biomass and cumulative yield increase with mowing height in Festuca pratensis irrespective of Epichloë symbiosis
Authors: Laihonen Miika , Rainio Kalle , Birge Traci , Saikkonen Kari , Helander Marjo , Fuchs Benjamin 
Published: December 2022

AbstractIncreasing agricultural soil carbon sequestration without compromising the productivity of the land is a key challenge in global climate change mitigation. The carbon mitigation potential of grass-based agriculture is particularly high because grasslands represent 70% of the world’s agricultural area. The root systems of grasses transfer large amounts of carbon to below-ground storage, and the carbon allocation to the roots is dependent on the grasses’ photosynthesizing shoot biomass. In a common-garden experiment, Festuca pratensis was used as a model species to study how mowing and weed control practices of perennial cool-season fodder grasses affect total yield and root biomass. Additionally, grass-associated Epichloë endophytes and soil residual glyphosate were tested for their effect on the total yield and root biomass alone or in interaction with mowing. The results demonstrate that elevating the cutting height increases both cumulative yield and root biomass in F. pratensis. Endophyte symbiosis increased the total yield, while glyphosate-based herbicide residues in the soil decreased the root biomass, which indicates a reduction of soil bound carbon sequestration. The findings demonstrate that carbon sequestration and yield quantities on farmed grasslands may significantly be improved by optimizing strategies for the use of plant protection products and adjustment of mowing intensity.
Bioengineered synthesis of phytochemical-adorned green silver oxide (Ag2O) nanoparticles via Mentha pulegium and Ficus carica extracts with high antioxidant, antibacterial, and antifungal activities
Authors: Shahzad Shirazi Maryam , Moridi Farimani Mahdi , Foroumadi Alireza , Ghanemi Kamal , Benaglia Maurizio , Makvandi Pooyan 
Published: December 2022

AbstractSilver oxide nanoparticles have various biomedical and pharmaceutical applications. However, conventional nanofabrication of Ag2O is associated with the use of toxic chemicals and organic solvents. To circumvent this hurdle, herein silver oxide quantum dots (Ag2O-QDs) were synthesized quickly (3 min) via the use of ultrasonic irradiation and plant-extract. Additionally, due to ultrasonic irradiation's effect on cell-wall destruction and augmentation of extraction efficiency, ultrasonic was also used in the preparation of Mentha pulegium and Ficus carica extracts (10 min, r.t) as natural eco-friendly reducing/capping agents. The UV–Vis result indicated a broad absorption peak at 400–500 nm. TEM/SEM analysis showed that ultrasound introduced a uniform spherical particle and significantly reduced particle size compared to the conventional heating method (∼ 9 nm vs. ∼ 100 nm). Silver and oxygen elements were found in the bio-synthesized Ag2O by EDS. The FTIR and phenol/flavonoid tests revealed the presence of phenol and flavonoid associated with the nanoparticles. Moreover, nanoparticles exhibited antioxidant/antibacterial/antifungal activities. The MIC and MBC results showed the Ag2O QDs synthesized with M. pulegium extract have the highest antibacterial activity against E. coli (MBC = MIC:15.6 ppm), which were significantly different from uncoated nanoparticles (MBC = MIC:500 ppm). The data reflects the role of phyto-synthesized Ag2O-QDs using ultrasonic-irradiation to develop versatile and green biomedical products.
Racial differences in prevalence and anatomical distribution of tarsal coalition
Authors: Park Jeong Jin , Seok Hyun Gyu , Woo In Ha , Park Chul Hyun 
Published: December 2022

AbstractPrevious studies have reported a prevalence of tarsal coalition of 0.03–13%. Calcaneonavicular coalition is known as main anatomical type, and the bilateral occurrence of tarsal coalition is known to be 50% or more. These are the results of studies on Caucasians, there have been few studies targeting large number of East Asians so far. We hypothesized that the prevalence and characteristics of tarsal coalition in East Asians might differ from those in Caucasians. The medical records of 839 patients who underwent bilateral computed tomography on foot and ankle in our hospital from January 2012 to April 2021 were retrospectively reviewed. The overall prevalence was 6.0%, talocalcaneal coalition was the most common anatomical type. The overall bilateral occurrence was 56.5%, talocalcaneal coalition had the highest bilateral occurrence (76.0%) among anatomical types. Isolated union of the posterior facet was the most common subtype of talocalcaneal coalition (43.2%). Talocalcaneal coalition had a significantly higher proportion of coalition-related symptomatic patients than calcaneonavicular coalition (p = 0.019). Our study showed a similar trend to other East Asian studies, confirming the existence of racial differences. The possibility of tarsal coalition in foot and ankle patients in East Asians should always be considered, and bilateral examination is essential for diagnosis.
Transcultural adaptation and psychometric validation of the Thai-Brief Resilient Coping Scale: a cross-sectional study during the coronavirus disease 2019 pandemic in Thailand
Authors: Nochaiwong Surapon , Ruengorn Chidchanok , Awiphan Ratanaporn , Phosuya Chabaphai , Ruanta Yongyuth , Kanjanarat Penkarn , Wongpakaran Nahathai , Wongpakaran Tinakon , Thavorn Kednapa 
Published: December 2022

AbstractThis study aimed to examine the transcultural adaptation, construct validity, and psychometric properties of the Thai-Brief Resilient Coping Scale (BRCS) among the general population and college students through the coronavirus disease 2019 (COVID-19) pandemic in Thailand. We invited the 4004 participants to complete sets of anchor-based measurement tools, including depressive symptoms, anxiety symptoms, perceived stress, well-being, and perceived social support. The scale factor structure of the Thai-BRCS was assessed using factor analysis, and nonparametric item response theory (IRT) analysis. The psychometric properties of the Thai-BRCS for validity (convergent and discriminant) and reliability (internal consistency and reproducibility) were assessed. Based on the construct validity testing, factor analysis, and nonparametric IRT analysis reaffirmed the unidimensionality with a one-factor structure of the Thai-BRCS version. For convergent validity, the scale was significantly correlated with all sets of anchor-based measurement tools (all P < 0.001). The discriminant validity was satisfactory with a group of medium and low resilience and the risk of adverse mental outcomes. For scale reliability, it revealed excellent internal consistency (alpha = 0.84, omega = 0.85) and reproducibility (intraclass correlation = 0.91). The Thai-BRCS version fulfills transcultural adaptation with satisfactory psychometric properties to measure psychological resilience in the Thai population during the COVID-19 pandemic.
Deep learning based sentiment analysis and offensive language identification on multilingual code-mixed data
Authors: Shanmugavadivel Kogilavani , Sathishkumar V. E. , Raja Sandhiya , Lingaiah T. Bheema , Neelakandan S. , Subramanian Malliga 
Published: December 2022

AbstractSentiment analysis is a process in Natural Language Processing that involves detecting and classifying emotions in texts. The emotion is focused on a specific thing, an object, an incident, or an individual. Although some tasks are concerned with detecting the existence of emotion in text, others are concerned with finding the polarities of the text, which is classified as positive, negative, or neutral. The task of determining whether a comment contains inappropriate text that affects either individual or group is called offensive language identification. The existing research has concentrated more on sentiment analysis and offensive language identification in a monolingual data set than code-mixed data. Code-mixed data is framed by combining words and phrases from two or more distinct languages in a single text. It is quite challenging to identify emotion or offensive terms in the comments since noise exists in code-mixed data. The majority of advancements in hostile language detection and sentiment analysis are made on monolingual data for languages with high resource requirements. The proposed system attempts to perform both sentiment analysis and offensive language identification for low resource code-mixed data in Tamil and English using machine learning, deep learning and pre-trained models like BERT, RoBERTa and adapter-BERT. The dataset utilized for this research work is taken from a shared task on Multi task learning DravidianLangTech@ACL2022. Another challenge addressed by this work is the extraction of semantically meaningful information from code-mixed data using word embedding. The result represents an adapter-BERT model gives a better accuracy of 65% for sentiment analysis and 79% for offensive language identification when compared with other trained models.
Podocyturia: an earlier biomarker of cardiovascular outcomes
Authors: Eid Assaad A. , Habib Robert H. , Chehab Omar , Al Jalbout Nour , Tamim Hani , Makki Maha , El Bejjani Martine , Lima Joao , Badr Kamal F. 
Published: December 2022

AbstractUrinary podocin and nephrin mRNAs (podocyturia), as candidate biomarkers of endothelial/podocyte injury, were measured by quantitative PCR in Type II diabetics with normal albumin excretion rates (AER) at baseline, at 3–4 years, and at 7 years. Development of cardiovascular disease (CVD) was collected as outcome. Visit 1 podocyturia was significantly higher in subjects who subsequently developed CVD versus those who did not. Visit 1 AER terciles exhibited similar time to CVD, in contrast with stepwise and substantial increases in CVD events predicted by Visit 1 podocyturia terciles. Covariate-adjusted hazard ratios were highest for podocin, intermediate for nephrin mRNAs, and lowest for AER. Podocyturia was also measured in patients with and without significant coronary obstruction, and in 480 normoalbuminuric subjects at the enrolment visit to the Multi-Ethnic Study of Atherosclerosis (MESA). Podocyturia > 3 × 106 copies was associated with presence of obstructive coronary artery disease. In the MESA population, Visit 1 podocyturia was significantly higher in men, subjects with elevated BMI, and those with Type II DM. Conclusions: Podocyturia may be an earlier predictor of cardiovascular events than moderate albuminuria; it is significantly higher in patients with obstructive coronary artery disease, and in subjects with established risk factors for CVD.