bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chest-scale self-compensated epidermal electronics for standard 6-precordial-lead ECG
Authors: Yin Lang , Wang Youhua , Zhan Jian , Bai Yunzhao , Hou Chao , Wu Junfeng , Huang Rong , Wang Yuzhou , Huang YongAn 
Published: May 2022

AbstractSix chest leads are the standardized clinical devices of diagnosing cardiac diseases. Emerging epidermal electronics technology shift the dangling wires and bulky devices to imperceptible wearing, achieving both comfortable experience and high-fidelity measuring. Extending small areas of current epidermal electronics to the chest scale requires eliminating interference from long epidermal interconnects and rendering the data acquisition (DAQ) portable. Herein, we developed a chest-scale epidermal electronic system (EES) for standard precordial-lead ECG and hydration monitoring, including the only μm-thick substrate-free epidermal sensing module and the soft wireless DAQ module. An electrical compensation strategy using double channels within the DAQ module and epidermal compensated branches (ECB) is proposed to eliminate unwanted signals from the long epidermal interconnects and to achieve the desired ECG. In this way, the EES works stably and precisely under different levels of exercise. Patients with sinus arrhythmias have been tested, demonstrating the prospect of EES in cardiac diseases.
Scalable estimation of pure multi-qubit states
Authors: Pereira Luciano , Zambrano Leonardo , Delgado Aldo 
Published: May 2022

AbstractWe introduce an inductiven-qubit pure-state estimation method based on projective measurements onmn + 1 separable bases ormentangled bases plus the computational basis, withm ≥ 2. The method exhibits a favorable scaling in the number of qubits compared to other estimation schemes. The use of separable bases makes our estimation method particularly well suited for applications in noisy intermediate-scale quantum computers, where entangling gates are much less accurate than local gates. Our method is also capable of estimating the purity of mixed states generated by the action of white noise on pure states. Monte Carlo simulations show that the method achieves a high estimation fidelity. Besides, the fidelity can be improved by increasingmabove 2. We experimentally demonstrate the method on the IBM’s quantum processors by estimating up to 10-qubit separable and entangled states. In particular, a 4-qubit GHZ is estimated with experimental fidelity of 0.875.
Mussel-inspired multifunctional surface through promoting osteogenesis and inhibiting osteoclastogenesis to facilitate bone regeneration
Authors: Wu Minhao , Zhang Yufeng , Wu Ping , Chen Feixiang , Yang Zhiqiang , Zhang Sheng , Xiao Lingfei , Cai Lin , Zhang Chong , Chen Yun , Deng Zhouming 
Published: May 2022

AbstractOsteogenesis and osteoclastogenesis are closely associated during the bone regeneration process. The development of multifunctional bone repair scaffolds with dual therapeutic actions (pro-osteogenesis and anti-osteoclastogenesis) is still a challenging task for bone tissue engineering applications. Herein, through a facile surface coating process, mussel-inspired polydopamine (PDA) is adhered to the surface of a biocompatible porous scaffold followed by the immobilization of a small-molecule activator (LYN-1604 (LYN)) and the subsequent in situ coprecipitation of hydroxyapatite (HA) nanocrystals. PDA, acting as an intermediate bridge, can provide strong LYN immobilization and biomineralization ability, while LYN targets osteoclast precursor cells to inhibit osteoclastic differentiation and functional activity, which endows LYN/HA-coated hybrid scaffolds with robust anti-osteoclastogenesis ability. Due to the synergistic effects of the LYN and HA components, the obtained three-dimensional hybrid scaffolds exhibited the dual effects of osteoclastic inhibition and osteogenic stimulation, thereby promoting bone tissue repair. Systematic characterization experiments confirmed the successful fabrication of LYN/HA-coated hybrid scaffolds, which exhibited an interconnected porous structure with nanoroughened surface topography, favorable hydrophilicity, and improved mechanical properties, as well as the sustained sequential release of LYN and Ca ions. In vitro experiments demonstrated that LYN/HA-coated hybrid scaffolds possessed satisfactory cytocompatibility, effectively promoting cell adhesion, spreading, proliferation, alkaline phosphatase activity, matrix mineralization, and osteogenesis-related gene and protein secretion, as well as stimulating angiogenic differentiation of endothelial cells. In addition to osteogenesis, the engineered scaffolds also significantly reduced osteoclastogenesis, such as tartrate-resistant acid phosphatase activity, F-actin ring staining, and osteoclastogenesis-related gene and protein secretion. More importantly, in a rat calvarial defect model, the newly developed hybrid scaffolds significantly promoted bone repair and regeneration. Microcomputed tomography, histological, and immunohistochemical analyses all revealed that the LYN/HA-coated hybrid scaffolds possessed not only reliable biosafety but also excellent osteogenesis-inducing and osteoclastogenesis-inhibiting effects, resulting in faster and higher-quality bone tissue regeneration. Taken together, this study offers a powerful and promising strategy to construct multifunctional nanocomposite scaffolds by promoting osteo/angiogenesis and suppressing osteoclastogenesis to accelerate bone regeneration.
Spatial distribution data of cultural sites from the Paleolithic to Bronze Age in Xinjiang, China
Authors: Tan Bo , Wang Hongwei , Wang Xiaoqin , Yi Suyan , Zhou Jing , Ma Chen , Dai Xinyan 
Published: May 2022

AbstractThe published map recording cultural sites in Xinjiang shows that there is a lack of data collection on the distribution of sites in the area, and no relevant data sets have been released. Existing written materials indicate that there are more cultural sites in this area. For this reason, we have collected and sorted out information. Our cultural site database provides the geographic location and corresponding geographic environment of each site in Xinjiang from the Paleolithic to the Bronze Age. The data record the human development and settlement process, settlement environment landscape characteristics, scale, type, quantity, and spatial distribution in Xinjiang in prehistoric China. These data not only are the basis for further understanding the spatial distribution of prehistoric humans in Xinjiang, but also provide references for understanding prehistoric human behavior and prehistoric man-land relationship, and the exchange of eastern and western civilizations. It is of great significance to modern social planning, site protection, and resource utilization.
Population-specific association of Clock gene polymorphism with annual cycle timing in stonechats
Authors: Justen Hannah , Hasselmann Timo , Illera Juan Carlos , Delmore Kira E. , Serrano David , Flinks Heiner , Senzaki Masayuki , Kawamura Kazuhiro , Helm Barbara , Liedvogel Miriam 
Published: May 2022

AbstractTiming is essential for survival and reproduction of organisms across the tree of life. The core circadian clock geneClkis involved in the regulation of annual timing events and shows highly conserved sequence homology across vertebrates except for one variable region of poly-glutamine repeats.Clkgenotype varies in some species with latitude, seasonal timing and migration. However, findings are inconsistent, difficult to disentangle from environmental responses, and biased towards high latitudes. Here we combine field data with a common-garden experiment to study associations ofClkpolymorphism with latitude, migration and annual-cycle timing within the stonechat species complex across its trans-equatorial distribution range. Our dataset includes 950 records from 717 individuals from nine populations with diverse migratory strategies. Gene diversity was lowest in resident African and Canary Island populations and increased with latitude, independently of migration distance. Repeat length and annual-cycle timing was linked in a population-specific way. Specifically, equatorial African stonechats showed delayed timing with longer repeat length for all annual-cycle stages. Our data suggest that at low latitudes with nearly constant photoperiod,Clkgenotype might orchestrate a range of consistent, individual chronotypes. In contrast, the influence ofClkon annual-cycle timing at higher latitudes might be mediated by its interactions with genes involved in (circadian) photoperiodic pathways.
Differential expression analysis of genes and long non-coding RNAs associated with KRAS mutation in colorectal cancer cells
Authors: Saliani Mahsa , Jalal Razieh , Javadmanesh Ali 
Published: May 2022

AbstractKRAS mutation is responsible for 40–50% of colorectal cancers (CRCs). RNA-seq data and bioinformatics methods were used to analyze the transcriptional profiles of KRAS mutant (mtKRAS) in comparison with the wild-type (wtKRAS) cell lines, followed by in-silico and quantitative real-time PCR (qPCR) validations. Gene set enrichment analysis showed overrepresentation of KRAS signaling as an oncogenic signature in mtKRAS. Gene ontology and pathway analyses on 600 differentially-expressed genes (DEGs) indicated their major involvement in the cancer-associated signal transduction pathways. Significant hub genes were identified through analyzing PPI network, with the highest node degree for PTPRC. The evaluation of the interaction between co-expressed DEGs and lncRNAs revealed 12 differentially-expressed lncRNAs which potentially regulate the genes majorly enriched in Rap1 and RAS signaling pathways. The results of the qPCR showed the overexpression of PPARG and PTGS2, and downregulation of PTPRC in mtKRAS cells compared to the wtKRAS one, which confirming the outputs of RNA-seq analysis. Further, significant upregualtion of miR-23b was observed in wtKRAS cells. The comparison between the expression level of hub genes and TFs with expression data of CRC tissue samples deposited in TCGA databank confirmed them as distinct biomarkers for the discrimination of normal and tumor patient samples. Survival analysis revealed the significant prognostic value for some of the hub genes, TFs, and lncRNAs. The results of the present study can extend the vision on the molecular mechanisms involved in KRAS-driven CRC pathogenesis.
Design of double sampling inspection plans for life tests under time censoring based on Pareto type IV distribution
Authors: Saranya C. R. , Vijayaraghavan R. , Sathya Narayana Sharma K. 
Published: May 2022

AbstractSampling inspection plans for life tests, called reliability sampling plans, are generally employed to determine the acceptance or non-acceptance of the lot(s) of finished products by performing tests on the sampled items, measuring the lifetime of the items and observing the number of failures of items. Lifetime of individual items is a prime quality characteristic that can be treated as a continuous random variable and can be modeled by an appropriate probability distribution. In this article, double sampling plans for life tests under time censoring with a provision to draw two random samples and to admit a maximum of one failure in the combined samples are formulated assuming that the lifetime random variable follows a Pareto type IV distribution. A methodical procedure for the selection of the plan parameters using reliable life criterion with the desired discrimination protecting the interests of the producer and the consumer in terms of the acceptable reliable life and unacceptable reliable life is evolved. The operating ratio is used as a measure of discrimination in designing the proposed reliability sampling plans.
Pd-containing magnetic periodic mesoporous organosilica nanocomposite as an efficient and highly recoverable catalyst
Authors: Neysi Maryam , Elhamifar Dawood 
Published: May 2022

AbstractA novel magnetic ionic liquid based periodic mesoporous organosilica supported palladium (Fe3O4@SiO2@IL-PMO/Pd) nanocomposite is synthesized, characterized and its catalytic performance is investigated in the Heck reaction. The Fe3O4@SiO2@IL-PMO/Pd nanocatalyst was characterized using FT-IR, PXRD, SEM, TEM, VSM, TG, nitrogen-sorption and EDX analyses. This nanocomposite was effectively employed as catalyst in the Heck reaction to give corresponding arylalkenes in high yield. The recovery test was performed to study the catalyst stability and durability under applied conditions.
Improving the design stage of air pollution studies based on wind patterns
Authors: Zabrocki Léo , Alari Anna , Benmarhnia Tarik 
Published: May 2022

AbstractA growing literature in economics and epidemiology has exploited changes in wind patterns as a source of exogenous variation to better measure the acute health effects of air pollution. Since the distribution of wind components is not randomly distributed over time and related to other weather parameters, multivariate regression models are used to adjust for these confounding factors. However, this type of analysis relies on its ability to correctly adjust for all confounding factors and extrapolate to units without empirical counterfactuals. As an alternative to current practices and to gauge the extent of these issues, we propose to implement a causal inference pipeline to embed this type of observational study within an hypothetical randomized experiment. We illustrate this approach using daily data from Paris, France, over the 2008–2018 period. Using the Neyman–Rubin potential outcomes framework, we first define the treatment of interest as the effect of North-East winds on particulate matter concentrations compared to the effects of other wind directions. We then implement a matching algorithm to approximate a pairwise randomized experiment. It adjusts nonparametrically for observed confounders while avoiding model extrapolation by discarding treated days without similar control days. We find that the effective sample size for which treated and control units are comparable is surprisingly small. It is however reassuring that results on the matched sample are consistent with a standard regression analysis of the initial data. We finally carry out a quantitative bias analysis to check whether our results could be altered by an unmeasured confounder: estimated effects seem robust to a relatively large hidden bias. Our causal inference pipeline is a principled approach to improve the design of air pollution studies based on wind patterns.
Pentosan polysulfate induces low-level persistent prion infection keeping measurable seeding activity without PrP-res detection in Fukuoka-1 infected cell cultures
Authors: Takatsuki Hanae , Imamura Morikazu , Mori Tsuyoshi , Atarashi Ryuichiro 
Published: May 2022

AbstractEach prion strain has its own characteristics and the efficacy of anti-prion drugs varies. Screening of prion disease therapeutics is typically evaluated by measuring amounts of protease-resistant prion protein (PrP-res). However, it remains unclear whether such measurements correlate with seeding activity, which is evaluated by real-time quaking-induced conversion (RT-QuIC). In this study, the effects of anti-prion compounds pentosan polysulfate (PPS), Congo red, and alprenolol were measured in N2a58 cells infected with Fukuoka-1 (FK1) or 22L strain. The compounds abolished PrP-res and seeding activity, except for N2a58/FK1 treated with PPS. Interestingly, the seeding activity of N2a58/FK1, which was reduced in the presence of PPS, was not lost and remained at low levels. However, upon removal of PPS, both were gradually restored to their original levels. These results indicate that low-level persistent prion infection keeping measurable seeding activity is induced by PPS in a strain-dependent manner. Furthermore, for protein misfolding cyclic amplification (PMCA), the anti-prion effect of PPS decreased in FK1 compared to 22L, suggesting that the differences occur at the level of the direct conversion. Our findings demonstrate that the advantages of RT-QuIC and PMCA can be exploited for more accurate assessment of therapeutic drug screening, reflecting strain differences.
The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study
Authors: Hartuis Sophie , Lavergne Rose-Anne , Nourrisson Céline , Verweij Jaco , Desoubeaux Guillaume , Lussac-Sorton Florian , Lemoine Jean-Philippe , Cateau Estelle , Jeddi Fakhri , Poirier Philippe , Le Pape Patrice , Morio Florent 
Published: May 2022

Objectives: We provide the first evaluation of the CE-IVD marked Novodiag® stool parasites assay (NVD), allowing rapid and high-plex detection of 26 distinct targets, encompassing protozoans, helminths and microsporidia in stool samples. Methods: A total of 254 samples (n = 205 patients) were prospectively processed by the NVD and our routine procedure (RP). Performances of the NVD were compared with RP. Samples only positive by the NVD assay were investigated by external PCR assays. Sensitivity and specificity (Se/Sp) and time from sample receipt to results were determined for each method. The NVD was also evaluated against 77 additional samples positive for a wide range of parasites. Results: Overall positivity rate was 16.9% for RP compared with 34% using the NVD assay, and 164 samples (66%) were negative by both methods. Only 30 positive samples (12%) showed full concordance between RP and NVD. Fifty-three discordant samples were sent for external investigations. Except for Giardia intestinalis and Trichuris spp., higher Se was observed for the NVD assay for Blastocystis spp. (100% vs. 63%), Dientamoeba fragilis (100% vs. 0%), Schistosoma spp. (100% vs. 17%), and Enterobius vermicularis (100% vs. 67%) but roughly similar to RP for the remaining parasites tested. False-positive results were identified for Blastocystis spp., G. intestinalis, and Trichuris spp. using the NVD assay. The NVD mostly provides a diagnosis on the day of sample receipt compared with a mean of three days with RP. Conclusions: Besides some limitations, the NVD is a new diagnostic strategy allowing rapid and high-plex detection of gastrointestinal parasites from unpreserved stools.
André-Marie Ampère et la naissance de l'électrodynamique
Authors: Fischer Hélène 
Published: May 2022

Dès 1801, Ampère a l'intuition fulgurante que magnétisme et courant électrique sont deux facettes différentes du même phénomène. En 1820, sa vie est bouleversée par l'expérience d'Œrsted, qui a observé qu'une aiguille aimantée est déviée sous l'action d'un courant électrique parcourant un fil placé à proximité.
Pour en proposer une explication, Ampère suppose avec audace l'existence de courants électriques dans les aimants. Avec frénésie, il n'a alors de cesse de concevoir de nouveaux dispositifs expérimentaux pour prouver la véracité de son hypothèse et établir la formule élémentaire et universelle qui fera de lui le « Newton de l'électricité ».
Digital and advanced electronic signature: the security function, especially in electronic commerce
Authors: Velentzas John , Kiriakoulis George , Broni Georgia , Kartalis Nick , Panou George , Fragulis George 
Published: May 2022

The purpose of Directive 1999/93 / EC on the Community framework for electronic signatures is to facilitate the use of electronic signatures. Digital signatures are incredibly important as they prevent fraud in e-commerce transactions. A legal framework for electronic signatures is established to ensure the smooth functioning of the internal market. This article aims to interpret the European framework for digital signatures.
Member States shall at least ensure that, when issuing a certificate, the certification body is liable for any damage caused to any entity or natural or legal person reasonably based on the certificate:
(a) the accuracy, at the time of issue, of all the information contained in the recognized certificate.
(b) ensure that, at the time of issue of the certificate, the signatories identified on the recognized certificate were the holders of the signature-creation data corresponding to the signature verification data referred to or specified in the certificate;
Member States shall at least ensure that the certified provider is liable for any damage caused to any entity or natural person who reasonably relies on the certificate, unless the certifying provider proves that it did not act negligently.
Member States shall ensure that a certified service provider may indicate in a recognized certificate restrictions on the use of that certificate.
Member States shall ensure that a certification-service-provider may indicate on the recognized certificate the limits on the amount of transactions for which the certificate may be used. For all of the above, the certification service provider is not liable for damages resulting from exceeding these limits.
Simethicone and N-acetyl cysteine combination as premedication before esophagogastroduodenoscopy: Double-blind randomized controlled trial
Authors: Krishnamurthy Veeraraghavan , Joseph Abel , Venkataraman Shreyas , Kurian George 
Published: May 2022

Abstract
Background and study aims Esophagogastroduodenoscopy (EGD), the most common method used for diagnosing upper gastrointestinal diseases, is often limited by the presence of foam and mucous. Thus, this study was designed to detect whether the combination of simethicone with N-acetyl cysteine (NAC) as premedication before EGD improves mucosal visualization.
Patients and methods A total of 768 consenting patients were enrolled in this prospective, double-blind, randomized placebo-controlled trial in four groups (A: simethicone + N-acetyl cysteine; B: simethicone alone; C: NAC alone; and D: placebo). After 20 minutes of consuming the corresponding solution, EGD was done and multiple images were obtained from the esophagus, stomach, and duodenum. Based on the various images obtained, the study parameters were calculated. Statistical Analysis Software (SAS) was used to analyze the results using Kruskal-Wallis with the Bonferroni correction method.
Results The study population consisted of 57 % men and the mean age was 44.18 years. Each group was randomized with 192 participants. Group A (combination of simethicone + NAC) premedication had the lowest total mucosal visibility score of 8.31, a significantly lower score for mucous/bubbles obscuring the vision, and less time to complete the procedure. Also, 81 % of the participants in group A did not require flushing to clear the mucous/bubbles. There were no side effects due to this premedication in any of the groups.
Conclusions  Using simethicone and NAC combined for premedication may improve the quality of EGD.