bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of naphthalimide-type chemsensor and its application in quality evaluation for polygonatum sibiricum Red
Authors: Wang Zhen , Sun Qiaoxu , Zhao Yuanyuan , Du Jiao , Wang Bin 
Published: August 2022

The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM. This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation. The HAN was synthesized based on a CuI-catalyzed cross-coupling reaction in water, and then employed as a novel chemosensor that reacts with reducing sugars. Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min. The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81. The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP. Additionally, the HAN displayed fluorescence enhancement through “push–pull” mode, and fluorescence decreased through “pull–pull” mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
Facile synthesis of nanosized Mn3O4 powder anodes for high capacity Lithium-Ion battery via flame spray pyrolysis
Authors: Wang Hao , Zhao Jiachang , Xie Dongmei , Huang Haiji , Rao Pinhua , Mao Jianfeng 
Published: August 2022

Mn3O4 powders with nanometer size are successfully synthesized by a simple one-step method via flame spray pyrolysis. The precursor droplet is generated by heating under high temperature flame with fixed flow rate, and the exothermic reaction is induced to form nanosized Mn3O4 powders. When used as anode material for lithium-ion battery, the Mn3O4 exhibits good cycling capacity and rate performance. It delivers a specific capacity of 1,182 mA h g−1 over 110 cycles at a current density of 200 mA g−1, and has a high capacity of 140 mA h g−1 at 5,000 mA g−1.
Z-score model of foetal ascending aorta diameter distensibility
Authors: Chen Fuli , Zeng Shi , Yi Aijiao , Chen Lihua , Zhou Dan , Liu Yushan , Yao Longmei 
Published: August 2022

ObjectiveThe purpose of this study is to establish Z-scores models of normal fetal ascending aorta diameter and diameter distensibility.MethodsThe maximum systolic diameter (Dmax), minimum diastolic diameter (Dmin), and diameter distensibility of the sinotubular junction were measured and taken as dependent variables in 490 normal fetuses at 18–40 gestational weeks, and gestational age (GA), biparietal diameter (BPD), and femoral length (FL) were taken as independent variables. The data were subjected to regression analysis, and the best-fitting equations for the dependent variables based on the independent variables were determined. The fitting equations were then applied to construct the Z-scores models.ResultsThe Dmax, Dmin and Diameter Distensibility in normal fetuses between 18 and 40 weeks of GA could be evaluated by utilizing the Z-scores models. Dmax and Dmin increased significantly with increasing GA, BPD, and FL. Diameter distensibility, assessed as (Dmax–Dmin)/Dmin, decreased significantly with increasing GA, BPD, and FL.ConclusionThe Z-scores are valuable, and can be utilized as a potent supplement to the conventional approach as they can indirectly reflect the development of fetal ascending aortic elastic property.
Sex difference in heart failure risk associated with febuxostat and allopurinol in gout patients
Authors: Cheng Ching-Lan , Yen Chi-Tai , Su Chien-Chou , Lee Cheng-Han , Huang Chien-Huei , Yang Yea-Huei Kao 
Published: August 2022

BackgroundGout or rapid reduction in serum uric acid level may increase the incidence of heart failure (HF). To compare the risk of HF between febuxostat and allopurinol in gout patients with coexisting cardiovascular (CV) diseases, the varying severity would be likely to confound the risk estimation. Gout and HF are both sex-related diseases, and the risk difference from the urate-lowering agents between women and men remains unknown.AimsTo evaluate the HF hospitalisations risk of febuxostat and allopurinol in gout patients in real-world settings.MethodsA population-based cohort enrolled patients with allopurinol or febuxostat initiation from 2011 to 2018. Participants were grouped into, without (low CV risk group) or with (high CV risk group) a history of recent major CV admission. The primary outcome was HF hospitalization. The secondary outcomes were composite CV events, all-cause mortality, and the cause of CV mortality. We used the ‘as-treated' analysis and Cox proportional hazards model after propensity score (PS) matching. Patients were further stratified into men and women to evaluate the gender differences.ResultsFebuxostat users had a significantly higher risk of HF hospitalization than allopurinol users in gout patients either with low CV risk [hazard ratio (HR) 1.39; 95% confidence interval (CI) 1.25–1.55] or high CV risk [HR 1.36; 95% CI 1.22–1.52]. Particularly, women with gout had a higher risk of HF hospitalization than men.ConclusionThe HF hospitalization risk was highest in gout women with high CV risk and febuxostat use. Monitoring of HF is warranted in these patients.
Intraoperative EMG recovery patterns and outcomes after RLN traction-related amplitude decrease during monitored thyroidectomy
Authors: Chiu Kuan-Lin , Lien Ching-Feng , Wang Chih-Chun , Wang Chien-Chung , Hwang Tzer-Zen , Shih Yu-Chen , Yu Wing-Hei Viola , Wu Che-Wei , Dionigi Gianlorenzo , Huang Tzu-Yen , Chiang Feng-Yu 
Published: August 2022

ObjectivesTraction injury is the most common type of recurrent laryngeal nerve (RLN) injury in thyroid surgery. Intraoperative neuromonitoring (IONM) facilitates early detection of adverse electromyography (EMG) effect, and this corrective maneuver can reduce severe and repeated nerve injury. This study aimed to evaluate intraoperative patterns and outcomes of EMG decrease and recovery by traction injury.Methods644 patients received nerve monitored thyroidectomy with 1142 RLNs at risk were enrolled. Intermittent IONM with stimulating dissecting instrument (real-time during surgical procedure) and trans-thyroid cartilage EMG recording method (without electrode malpositioning issue) were used for nerve stimulation and signal recording. When an EMG amplitude showed a decrease of &gt;50% during RLN dissection, the surgical maneuver was paused immediately. Nerve dissection was restarted when the EMG amplitude was stable.Results44/1142 (3.9%) RLNs exhibited a &gt;50% EMG amplitude decrease during RLN dissection and all (100%) showed gradual progressive amplitude recovery within a few minutes after releasing thyroid traction (10 recovered from LOS; 34 recovered from a 51-90% amplitude decrease). Three EMG recovery patterns were noted, A-complete EMG recovery (n=14, 32%); B-incomplete EMG recovery with an injury point (n=16, 36%); C-incomplete EMG recovery without an injury point (n=14, 32%). Patients with postoperative weak or fixed vocal cord mobility in A, B, and C were 0(0%), 7(44%), and 2(14%), respectively. Complete EMG recovery was found in 14 nerves, and incomplete recovery was found in another 30 nerves. Temporary vocal cord palsy was found in 6 nerves due to unavoidable repeated traction.ConclusionEarly detection of traction-related RLN amplitude decrease allows monitoring of intraoperative EMG signal recovery during thyroid surgery. Different recovery patterns show different vocal cord function outcomes. To elucidate the recovery patterns can assist surgeons in the intraoperative decision making and postoperative management.
Global signalling network analysis of luminal T47D breast cancer cells in response to progesterone
Authors: Wright Roni H. G. , Vastolo Viviana , Oliete Javier Quilez , Carbonell-Caballero José , Beato Miguel 
Published: August 2022

BackgroundBreast cancer cells enter into the cell cycle following progestin exposure by the activation of signalling cascades involving a plethora of enzymes, transcription factors and co-factors that transmit the external signal from the cell membrane to chromatin, ultimately leading to a change of the gene expression program. Although many of the events within the signalling network have been described in isolation, how they globally team up to generate the final cell response is unclear.MethodsIn this study we used antibody microarrays and phosphoproteomics to reveal a dynamic global signalling map that reveals new key regulated proteins and phosphor-sites and links between previously known and novel pathways. T47D breast cancer cells were used, and phospho-sites and pathways highlighted were validated using specific antibodies and phenotypic assays. Bioinformatic analysis revealed an enrichment in novel signalling pathways, a coordinated response between cellular compartments and protein complexes.ResultsDetailed analysis of the data revealed intriguing changes in protein complexes involved in nuclear structure, epithelial to mesenchyme transition (EMT), cell adhesion, as well as transcription factors previously not associated with breast cancer cell proliferation. Pathway analysis confirmed the key role of the MAPK signalling cascade following progesterone and additional hormone regulated phospho-sites were identified. Full network analysis shows the activation of new signalling pathways previously not associated with progesterone signalling in T47D breast cancer cells such as ERBB and TRK. As different post-translational modifications can mediate complex crosstalk mechanisms and massive PARylation is also rapidly induced by progestins, we provide details of important chromatin regulatory complexes containing both phosphorylated and PARylated proteins.ConclusionsThis study contributes an important resource for the scientific community, as it identifies novel players and connections meaningful for breast cancer cell biology and potentially relevant for cancer management.
Genetics: Is LADA just late onset type 1 diabetes?
Authors: Hernández M. , Nóvoa-Medina Y. , Faner R. , Palou E. , Esquerda A. , Castelblanco E. , Wägner A. M. , Mauricio D. 
Published: August 2022

BackgroundThere is a controversy regarding Latent Autoimmune Diabetes in Adults (LADA) classification and whether it should be considered a slowly progressing form of type 1 (T1) diabetes (DM) or a distinct type of DM altogether.MethodsThis cross-sectional study assessed major genes associated with T1DM (class II HLA, PTPN22 [rs2476601] and INS [rs689]) in patients with LADA, as compared with participants with T1DM (stratified according to age of diagnosis before or after 30) and T2DM. HLA genotyping of the DRB1, DQA1 and DQB1 loci was performed by reverse PCR sequence-specific oligonucleotides. HLA haplotypes were assigned according to those most frequently described in the European population. INS and PTPN22 SNPs were genotyped by real-time PCR.ResultsA total of 578 participants were included: 248 with T1DM (70 diagnosed after the age of 30), 256 with T2DM and 74 with LADA. High risk HLA alleles were significantly more frequent in LADA than in T2DM, whereas the opposite was true for protective alleles. We found a lower frequency of the high-risk DRB1*04-DQB1*03:02-DQA1*03:01 haplotype in LADA (21.1%) than in the overall T1DM (34.7%) (p&lt;0.05), whereas no differences were found between these groups for DRB1*03-DQB1*02:01-DQA1*05:01 or for protective alleles. Only 12% the overall T1DM group had no risk alleles vs 30% of LADA (p&lt;0.0005). However, HLA allele distribution was similar in LADA and T1DM diagnosed after the age of 30. A total of 506 individuals (195 with T1DM [21 diagnosed after age 30] 253 with T2DM and 58 with LADA) were genotyped for the PTPN22 and INS SNPs. The G/A genotype of the PTPN22 rs2476601 was more frequent and the T/T genotype of the INS SNP rs689 was less frequent in T1DM compared to LADA. We did not find any significant differences in the frequency of the mentioned SNPs between LADA and T2DM, or between LADA and T1DM diagnosed after the age of 30.ConclusionIn this relatively small cross-sectional study, the genetic profile of subjects with LADA showed a similar T1DM-related risk allele distribution as in participants with T1DM diagnosed after the age of 30, but fewer risk alleles than those diagnosed before 30. Differences were present for HLA, as well as PTPN22 and INS genes.
Effect of Chinese herbal compounds on ocular fundus signs and vision in conventional treated-persons with non-proliferative diabetic retinopathy: A systematic review and meta-analysis
Authors: Li Xiaodong , Zhang Jiaqi , He Runxi , Su Xiaojuan , Li Zhilin , Xie Xuejun 
Published: August 2022

BackgroundChanges in fundus signs and loss of visual acuity are an important basis for screening and treating diabetic patients with retinopathy, and conventional Western medicine is moderately effective in treating diabetic retinopathy(DR),To systematically evaluate the effectiveness and safety of Chinese herbal compounds(CHCs) in the combined treatment of diabetic retinopathy.MethodSix electronic databases, including PubMed, were searched to screen eligible literature. Randomized controlled trials of non-proliferative diabetic retinopathy(NPDR) were included, in which the control group was treated with conventional Western-based drugs or retinal laser photocoagulation, and the intervention group was treated with CHCs in combination based on the control group.The Cochrane Risk of Bias Assessment Tool was used to evaluate the quality of the literature, and the RevMan 5.4 software was used for statistical analysis.ResultsCompared with Conventional group alone,CHCs group was superior at improving clinical efficacy [RR=1.29, 95%CI=(1.23, 1.36),P&lt;0.01] and best corrected visual acuity(BCVA) [MD=0.10,95%CI=(0.09,0.12),P&lt;0.01],it was also superior at reducing the number of microangiomas [MD=-2.37, 95%CI=(-3.26, -1.49),P&lt;0.01], microangioma volume [MD=-4.72, 95%CI=(-5.14, -4.29), P&lt;0.01], hemorrhagic spots [MD=-2.05, 95%CI=(-2.51,-1.59), P&lt;0.01], hemorrhagic area [MD=-0.76, 95%CI=(-1.06, -0.47), P&lt;0.01], hard exudates [MD= -1.86, 95%CI=(-2.43, -1.28), P&lt;0.01], cotton lint spots [MD= -0.93, 95%CI= (-1.31, -0.55), P&lt;0.01], central macular thickness(CMT) [SMD=-1.52, 95%CI=(-1.85, -1.19),P&lt;0.01], Chinese medicine evidence score [SMD=-1.33,95%CI=(-1.58, -1.08),P&lt;0.01], fasting blood glucose (FBG) [SMD=-0.47, 95%CI=(-0.61,-0.33),P&lt;0.01], 2h postprandial blood glucose(2hPBG) [SMD=-0.87, 95% CI=(-1.06, -0.67), P&lt;0.01], glycosylated hemoglobin (HbAlc) [SMD=-0.76, 95%CI=(-1.16, -0.3),P&lt;0.01], total cholesterol(TC) [SMD=-0.33,95%CI=(-0.51,-0.16),P&lt;0.01],and CHCs group with less adverse events occurred [RR=0.46, 95%CI=(0.29, 0.74),P&lt;0.01].ConclusionCHCs combined with conventional medicine for NPDR has better clinical efficacy and higher safety, but the above findings need further validation in more large sample, multicenter, and low-bias RCTs due to the limitation of the quality and quantity of included literature.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42022342137.
Identification of an autophagy-related 12-lncRNA signature and evaluation of NFYC-AS1 as a pro-cancer factor in lung adenocarcinoma
Authors: Tong Fang , Xu Lifa , Xu Sheng , Zhang Mingming 
Published: August 2022

Objective: To develop an autophagy-related lncRNA-based risk signature and corresponding nomogram to predict overall survival (OS) for LUAD patients and investigate the possible meaning of screened factors.Methods: Differentially expressed lncRNAs and autophagy genes were screened between normal and LUAD tumor samples from the TCGA LUAD dataset. Univariate and multivariate Cox regression analyses were performed to construct the lncRNA-based risk signature and nomogram incorporating clinical information. Then, the accuracy and sensitivity were confirmed by the AUC of ROC curves in both training and validation cohorts. qPCR, immunoblot, shRNA, and ectopic expression were used to verify the positive regulation of NFYC-AS1 on BIRC6. CCK-8, immunofluorescence, and flow cytometry were used to confirm the influence of NFYC-AS1 on cell proliferation, autophagy, and apoptosis via BIRC6.Results: A 12-lncRNA risk signature and a nomogram combining related clinical information were constructed. Furthermore, the abnormal increase of NFYC-AS1 may promote LUAD progression through the autophagy-related gene BIRC6.Conclusion: 12-lncRNA signature may function as a predictive marker for LUAD patients, and NFYC-AS1 along with BIRC6 may function as carcinogenic factors in a combinatorial manner.
Inflammatory aging clock: A cancer clock to characterize the patients’ subtypes and predict the overall survival in glioblastoma
Authors: Zhu Lei , Wang Feng , Huang Jiannan , Wang He , Wang Guangxue , Jiang Jianxin , Li Qinchuan 
Published: August 2022

Background: Many biological clocks related to aging have been linked to the development of cancer. A recent study has identified that the inflammatory aging clock was an excellent indicator to track multiple diseases. However, the role of the inflammatory aging clock in glioblastoma (GBM) remains to be explored. This study aimed to investigate the expression patterns and the prognostic values of inflammatory aging (iAge) in GBM, and its relations with stem cells.Methods: Inflammation-related genes (IRG) and their relations with chronological age in normal samples from the Cancer Genome Atlas (TCGA) were identified by the Spearman correlation analysis. Then, we calculated the iAge and computed their correlations with chronological age in 168 patients with GBM. Next, iAge was applied to classify the patients into high- and low-iAge subtypes. Next, the survival analysis was performed. In addition, the correlations between iAge and stem cell indexes were evaluated. Finally, the results were validated in an external cohort.Results: Thirty-eight IRG were significantly associated with chronological age (|coefficient| &gt; 0.5), and were used to calculate the iAge. Correlation analysis showed that iAge was positively correlated with chronological age. Enrichment analysis demonstrated that iAge was highly associated with immune cells and inflammatory activities. Survival analysis showed the patients in the low-iAge subtype had significantly better overall survival (OS) than those in the high-iAge subtype (p &lt; 0.001). In addition, iAge outperformed the chronological age in revealing the correlations with stem cell stemness. External validation demonstrated that iAge was an excellent method to classify cancer subtypes and predict survival in patients with GBM.Conclusions: Inflammatory aging clock may be involved in the GBM via potential influences on immune-related activities. iAge could be used as biomarkers for predicting the OS and monitoring the stem cell.
An enhanced level of VCAM in transplant preservation fluid is an independent predictor of early kidney allograft dysfunction
Authors: Baboudjian Michael , Gondran-Tellier Bastien , Boissier Romain , Ancel Patricia , Marjollet Juline , Lyonnet Luc , François Pauline , Sabatier Florence , Lechevallier Eric , Dutour Anne , Paul Pascale 
Published: August 2022

BackgroundWe aimed to evaluate whether donor-related inflammatory markers found in kidney transplant preservation fluid can associate with early development of kidney allograft dysfunction.MethodsOur prospective study enrolled 74 consecutive donated organs who underwent kidney transplantation in our center between September 2020 and June 2021. Kidneys from 27 standard criteria donors were allocated to static cold storage and kidneys from 47 extended criteria donors to hypothermic machine perfusion. ELISA assessment of inflammatory biomarkers (IL-6, IL6-R, ICAM, VCAM, TNFα, IFN-g, CXCL1 and Fractalkine) was analyzed in view of a primary endpoint defined as the occurrence of delayed graft function or slow graft function during the first week following transplantation.ResultsSoluble VCAM levels measured in transplant conservation fluid were significantly associated with recipient serum creatinine on day 7. Multivariate stepwise logistic regression analysis identified VCAM as an independent non-invasive predictor of early graft dysfunction, both at 1 week (OR: 3.57, p = .04, 95% CI: 1.06-12.03) and 3 Months (OR: 4.039, p = .034, 95% CI: 1.11-14.73) after transplant surgery.ConclusionsThis prospective pilot study suggests that pre-transplant evaluation of VCAM levels could constitute a valuable indicator of transplant health and identify the VCAM-CD49d pathway as a target to limit donor-related vascular injury of marginal transplants.
Quantifying baseline costs and cataloging potential optimization strategies for kelp aquaculture carbon dioxide removal
Authors: Coleman Struan , Dewhurst Tobias , Fredriksson David W. , St. Gelais Adam T. , Cole Kelly L. , MacNicoll Michael , Laufer Eric , Brady Damian C. 
Published: August 2022

To keep global surface warming below 1.5°C by 2100, the portfolio of cost-effective CDR technologies must expand. To evaluate the potential of macroalgae CDR, we developed a kelp aquaculture bio-techno-economic model in which large quantities of kelp would be farmed at an offshore site, transported to a deep water “sink site”, and then deposited below the sequestration horizon (1,000 m). We estimated the costs and associated emissions of nursery production, permitting, farm construction, ocean cultivation, biomass transport, and Monitoring, Reporting, and Verification (MRV) for a 1,000 acre (405 ha) “baseline” project located in the Gulf of Maine, USA. The baseline kelp CDR model applies current systems of kelp cultivation to deep water (100 m) exposed sites using best available modeling methods. We calculated the levelized unit costs of CO2eq sequestration (LCOC; $ tCO2eq-1). Under baseline assumptions, LCOC was $17,048 tCO2eq-1. Despite annually sequestering 628 tCO2eq within kelp biomass at the sink site, the project was only able to net 244 C credits (tCO2eq) each year, a true sequestration “additionality” rate (AR) of 39% (i.e., the ratio of net C credits produced to gross C sequestered within kelp biomass). As a result of optimizing 18 key parameters for which we identified a range within the literature, LCOC fell to $1,257 tCO2eq-1 and AR increased to 91%, demonstrating that substantial cost reductions could be achieved through process improvement and decarbonization of production supply chains. Kelp CDR may be limited by high production costs and energy intensive operations, as well as MRV uncertainty. To resolve these challenges, R&amp;D must (1) de-risk farm designs that maximize lease space, (2) automate the seeding and harvest processes, (3) leverage selective breeding to increase yields, (4) assess the cost-benefit of gametophyte nursery culture as both a platform for selective breeding and driver of operating cost reductions, (5) decarbonize equipment supply chains, energy usage, and ocean cultivation by sourcing electricity from renewables and employing low GHG impact materials with long lifespans, and (6) develop low-cost and accurate MRV techniques for ocean-based CDR.