bluuebunny/crossref_metadata_2025_split huggingface dataset:
The aberrant dynamic amplitude of low-frequency fluctuations in melancholic major depressive disorder with insomnia
Authors: Deng Zijing , Jiang Xiaowei , Liu Wen , Zhao Wenhui , Jia Linna , Sun Qikun , Xie Yu , Zhou Yifang , Sun Ting , Wu Feng , Kong Lingtao , Tang Yanqing 
Published: August 2022

BackgroundInsomnia is considered one of the manifestations of sleep disorders, and its intensity is linked to the treatment effect or suicidal thoughts. Major depressive disorder (MDD) is classified into various subtypes due to heterogeneous symptoms. Melancholic MDD has been considered one of the most common subtypes with special sleep features. However, the brain functional mechanisms in melancholic MDD with insomnia remain unclear.Materials and methodsMelancholic MDD and healthy controls (HCs, n = 46) were recruited for the study. Patients were divided into patients with melancholic MDD with low insomnia (mMDD-LI, n = 23) and patients with melancholic MDD with high insomnia (mMDD-HI, n = 30), according to the sleep disturbance subscale of the 17-item Hamilton Depression Rating Scale. The dynamic amplitude of low-frequency fluctuation was employed to investigate the alterations of brain activity among the three groups. Then, the correlations between abnormal dALFF values of brain regions and the severity of symptoms were investigated.ResultsLower dALFF values were found in the mMDD-HI group in the right middle temporal gyrus (MTG)/superior temporal gyrus (STG) than in the mMDD-LI (p = 0.014) and HC groups (p &lt; 0.001). Melancholic MDD groups showed decreased dALFF values than HC in the right middle occipital gyri (MOG)/superior occipital gyri (SOG), the right cuneus, the bilateral lingual gyrus, and the bilateral calcarine (p &lt; 0.05). Lower dALFF values than HC in the left MOG/SOG and the left cuneus in melancholic MDD groups were found, but no significant difference was found between the mMDD-LI group and HC group (p = 0.079). Positive correlations between the dALFF values in the right MTG/STG and HAMD-SD scores (the sleep disturbance subscale of the HAMD-17) in the mMDD-HI group (r = 0.41, p = 0.042) were found. In the pooled melancholic MDD, the dALFF values in the right MOG/SOG and the right cuneus (r = 0.338, p = 0.019), the left MOG/SOG and the left cuneus (r = 0.299, p = 0.039), and the bilateral lingual gyrus and the bilateral calcarine (r = 0.288, p = 0.047) were positively correlated with adjusted HAMD scores.ConclusionThe occipital cortex may be related to depressive symptoms in melancholic MDD. Importantly, the right MTG/STG may play a critical role in patients with melancholic MDD with more severe insomnia.
Abnormal resting-state functional connectivity of hippocampal subregions in children with primary nocturnal enuresis
Authors: Zhong Shaogen , Zhang Lichi , Wang Mengxing , Shen Jiayao , Mao Yi , Du Xiaoxia , Ma Jun 
Published: August 2022

ObjectivePrevious neuroimaging studies have shown abnormal brain-bladder control network in children with primary nocturnal enuresis (PNE). The hippocampus, which has long been considered to be an important nerve center for memory and emotion, has also been confirmed to be activating during micturition in several human imaging studies. However, few studies have explored hippocampus-related functional networks of PNE in children. In this study, the whole resting-state functional connectivity (RSFC) of hippocampus was investigated in children with PNE.MethodsFunctional magnetic resonance imaging data of 30 children with PNE and 29 matched healthy controls (HCs) were analyzed in our study. We used the seed-based RSFC method to evaluate the functional connectivity of hippocampal subregions defined according to the Human Brainnetome Atlas. Correlation analyses were also processed to investigate their relationship with disease duration time, bed-wetting frequency, and bladder volume.ResultsCompared with HCs, children with PNE showed abnormal RSFC of the left rostral hippocampus (rHipp) with right fusiform gyrus, right Rolandic operculum, left inferior parietal lobule, and right precentral gyrus, respectively. Moreover, decreased RSFC of the left caudal hippocampus (cHipp) with right fusiform gyrus and right supplementary motor area was discovered in the PNE group. There were no significant results in the right rHipp and cHipp seeds after multiple comparison corrections. In addition, disease duration time was negatively correlated with RSFC of the left rHipp with right Rolandic operculum (r = −0.386, p = 0.035, uncorrected) and the left cHipp with right fusiform gyrus (r = −0.483, p = 0.007, uncorrected) in the PNE group, respectively. In the Receiver Operating Characteristic (ROC) analysis, all the above results of RSFC achieved significant performance.ConclusionsTo our knowledge, this is the first attempt to examine the RSFC patterns of hippocampal subregions in children with PNE. These findings indicated that children with PNE have potential dysfunctions in the limbic network, sensorimotor network, default mode network, and frontoparietal network. These networks may become less efficient with disease duration time, inducing impairments in brain-bladder control, cognition, memory, and emotion. Further prospective research with dynamic observation of brain imaging, bladder function, cognition, memory, and emotion is warranted.
Application of metagenomic next-generation sequencing in the diagnosis and treatment of recurrent urinary tract infection in kidney transplant recipients
Authors: Duan Wenjing , Yang Yongguang , Zhao Jingge , Yan Tianzhong , Tian Xiangyong 
Published: August 2022

BackgroundRapid and accurate pathogen diagnosis is an urgent unmet clinical need for recurrent urinary tract infection (RUTI) in kidney transplant recipients (KTRs). Metagenomic next-generation sequencing (mNGS) may offer another strategy for diagnosing uropathogens but remains to be studied.MethodsNineteen KTRs with RUTI were collected in this study. The uropathogens were detected and compared by mNGS and urine culture, respectively. Modifications of the anti-infection strategy were also assessed.ResultsRich and diverse pathogens were revealed by mNGS. mNGS was significantly higher than culture in total positive rate (100.0% vs. 31.6%; p &lt; 0.01) and in identification rates for bacteria (89.5% vs. 31.6%; p &lt; 0.01), for viruses (57.9% vs. 0; p &lt; 0.01), and for fungi (42.1% vs. 0; p &lt; 0.01), respectively. mNGS identified a significantly higher proportion of mixed infections than culture (89.5% vs. 10.5%; p &lt; 0.01). The anti-infection therapies were adjusted in two (33.3%) and 12 (76.9%) cases guided by culture and mNGS, respectively.ConclusionmNGS has more remarkable etiological diagnostic performance compared with urine culture for KTRs with RUTI to guide anti-infection strategies and, in turn, protect the graft.
Characteristics of Long-COVID care centers in Italy. A national survey of 124 clinical sites
Authors: Floridia Marco , Grassi Tiziana , Giuliano Marina , Tiple Dorina , Pricci Flavia , Villa Marika , Silenzi Andrea , Onder Graziano 
Published: August 2022

BackgroundDespite the growing clinical relevance of Long-COVID, there is minimal information available on the organizational response of health services to this condition.MethodsA national online survey of centers providing assistance for Long-COVID was implemented. Information collected included date of start of activity, target population, mode of assistance and of referral, type and number of specialists available, diagnostic and instrumental tests, use of telemedicine and of specific questionnaires.ResultsBetween February and May 2022, 124 centers completed the survey. Half of them were situated in northern Italy. Most (88.9%) provided assistance through either outpatient visits or day hospital services. Eleven (8.9%) assisted pediatric patients. Access to centers included scheduled visits for previously hospitalized patients (67.7%), referral from primary care (62.1%), from other specialists (46.8%), and, less commonly, from other services. Almost half of the centers (46.3%) started their activity early in the pandemics (March-September 2020). Almost all (93.5%) communicated with primary care physicians, and 21.8% used telemedicine tools. The mean number of patients followed was 40 per month (median 20, IQR 10-40). In most cases, the center coordinator was a specialist in respiratory diseases (30.6%), infectious diseases (28.2%), or internal medicine (25.0%). At least half of the centers had specialistic support in cardiology, respiratory diseases, radiology, infectious diseases, neurology and psychology, but roughly one quarter of centers had just only one (14.5%) or two (9.7%) specialists available. The clinical assessment was usually supported by a wide range of laboratory and instrumental diagnostics and by multidimensional evaluations.ConclusionsMost of the centers had an articulate and multidisciplinary approach to diagnosis and care of Long-COVID. However, a minority of centers provided only single or dual specialistic support. These findings may be of help in defining common standards, interventions and guidelines that can reduce gaps and heterogeneity in assistance to patients with Long-COVID.
Risk factors of falls in elderly patients with visual impairment
Authors: Ouyang Shuyi , Zheng Chunwen , Lin Zhanjie , Zhang Xiaoni , Li Haojun , Fang Ying , Hu Yijun , Yu Honghua , Wu Guanrong 
Published: August 2022

ObjectiveTo examine the risk factors for falls in elderly patients with visual impairment (VI) and assess the predictive performance of these factors.MethodsBetween January 2019 and March 2021, a total of 251 elderly patients aged 65–92 years with VI were enrolled and then prospectively followed up for 12 months to evaluate outcomes of accidental falls via telephone interviews. Information of demographics and lifestyle, gait and balance deficits, and ophthalmic and systemic conditions were collected during baseline visits. Forward stepwise multivariable logistic regression analysis was performed to identify independent risk factors of falls in elderly patients with VI, and a derived nomogram was constructed.ResultsA total of 143 falls were reported in 251 elderly patients during follow-up, with an incidence of 56.97%. The risk factors for falls in elderly patients with VI identified by multivariable logistic regression were women [odds ratio (OR), 95% confidence interval (CI): 2.71, 1.40–5.27], smoking (3.57, 1.34–9.48), outdoor activities/3 months (1.31, 1.08–1.59), waking up frequently during the night (2.08, 1.15–3.79), disorders of balance and gait (2.60, 1.29–5.24), glaucoma (3.12, 1.15–8.44), other retinal degenerations (3.31, 1.16–9.43) and best-corrected visual acuity (BCVA) of the better eye (1.79, 1.10–2.91). A nomogram was developed based on the abovementioned multivariate analysis results. The area under receiver operating characteristic curve of the predictive model was 0.779.ConclusionsGender, smoking, outdoor activities, waking up at night, disorders of balance and gait, glaucoma, other retinal degeneration and BCVA of the better eye were independent risk factors for falls in elderly patients with VI. The predictive model and derived nomogram achieved a satisfying prediction of fall risk in these individuals.
“There is always a waitlist”: The experiences of perinatal women randomized to a waitlist condition in a trial evaluating a novel online self-directed intervention for perinatal anxiety
Authors: Kristjanson Amanda J. , Hardman Madison P. , Penner Kailey E. , Gornik Megan E. , Pryor Teaghan A. M. , Petty Sarah K. , Alcolado Gillian M. , Furer Patricia , Reynolds Kristin A. 
Published: August 2022

BackgroundPregnant and postpartum women are at a heightened risk for the development or worsening of mental health problems, with elevated rates of mood and anxiety disorders noted across studies. Timely access to mental health supports is critical during the perinatal period (spanning pregnancy to 1 year postpartum), to mitigate potential negative impacts on mother and child. In general adult populations, a small body of research has highlighted the association between being waitlisted for mental health services with a deterioration in mental health. Given the influx of changes experienced in the perinatal period, this population may face unique challenges around being waitlisted. There is a lack of research exploring the experiences of perinatal women waitlisted for psychological services. The current study seeks to understand the experiences of perinatal women randomized to the waitlist condition of a randomized controlled trial.MethodsN= 20 participants (4 pregnant, 16 postpartum) from Central Canada who were enrolled in a novel online self-directed intervention for perinatal anxiety completed a virtual qualitative interview concerning their experience during the 6-week waitlist period for this randomized controlled trial. Interviews were audio-recorded, transcribed, and analyzed according to reflexive thematic analysis.ResultsSeven main themes were identified, depicting the waitlist experiences of perinatal participants: (a) “There is always a waitlist” (sub-themes: service availability, need to seek out services pre-emptively); (b) Timing of support access is vital during the perinatal period (sub-themes: prenatal, postpartum); (c) Responses to being waitlisted (sub-themes: disappointment, neutral, relief, “there's probably somebody that needs it more than I do”); (d) Identification of helpful supports during the waitlist period (sub-themes: formal supports, informal supports); (e) Connections with research team (sub-themes: communication, resource provision); (f) Impact of waitlist experience on desire to start program (sub-themes: excitement, “out of sight, out of mind,” nervousness); and (g) Improving the waitlist experience (sub-themes: communication, resource provision, triaging).DiscussionFindings highlight the need for timely access to mental health supports during the perinatal period and offer several recommendations for improving the waitlist experience, including providing more frequent waitlist status updates, providing more direct access to intermediate interventions, and triaging patients based on clinical need.
Pyroptosis-related gene-based prognostic signature for predicting the overall survival of oral squamous cell carcinoma patients
Authors: Zeng Deliang , Wang Xiao , Zhang Shuning , Zheng Ao , Huang Qingfeng , Cao Lingyan 
Published: August 2022

PurposeOral squamous cell carcinoma (OSCC) is the most common oral cancer worldwide. Pyroptosis is a type of programmed cell death mediated by caspase, accompanied by an inflammatory response, and plays an important role in cancer progression. The purpose of this study was to explore and identify potential biomarkers and further elucidate the potential role of cell pyroptosis in OSCC.MethodsWe regarded the samples from The Cancer Genome Atlas database as a training dataset, screened differentially expressed genes (DEGs), and further screened out OSCC phenotypic characteristic genes by using weighted gene co-expression network analysis. The analysis of 42 known pyroptosis-related genes showed that Psuch genes were widely expressed, mutated, and methylated in OSCC samples.ResultsThrough correlation analysis, we identified our OSCC pyroptosis-related DEGs. To further evaluate the prognostic value of pyroptosis-related regulators, we constructed a seven gene-based prognostic signature using Cox univariate analysis and least absolute shrinkage and selection operator Cox regression analysis. Meanwhile, we found that patients in the low-risk group had higher immune infiltration. Moreover, our results also indicated significant differences in sensitivity to cisplatin and gefitinib between the high-risk and low-risk groups.ConclusionOur study successfully constructed the pyroptosis-related prognostic signature, which might play a potential prediction role in OSCC prognosis. Our findings also suggested that pyroptosis-related regulators might be novel biomarkers for tumor diagnosis and treatment in OSCC.
Retroperitoneoscopic pyeloplasty with simultaneous pyelolithotomy using a flexible cystoscope: Our initial experience at a single centre
Authors: Zhang Fei , Wang Li , Gao ZheBin , Yang HouMeng 
Published: August 2022

PurposeWe present our experience with retroperitoneoscopic pyeloplasty with simultaneous pyelolithotomy using a flexible cystoscope in patients with ureteropelvic junction obstruction(UPJO) complicated with kidney stones.Materials and MethodsThe records of 37 patients who underwent retroperitoneoscopic pyeloplasty with simultaneous pyelolithotomy using a flexible cystoscope to manage UPJO complicated with kidney stones from July 2015 to December 2020 were retrospectively reviewed. All patients underwent one-stage retroperitoneoscopic pyeloplasty combined with flexible cystoscopic pyelolithotomy. The operative time, blood volume, stone clearance rate, length of hospital stay, complications and follow-up events were recorded.ResultsThe operation went smoothly in all 37 patients. The mean operative time was 148.4 ± 24.2 min. The mean intraoperative blood loss volume was 54.3 ± 20.5 ml. The mean hospitalization time was 10.6 ± 3.7 days. The stone clearance rate was 81.08%. The mean follow-up period was 23.5 months (range 12–53 months). Hydronephrosis was significantly decreased in 33 of the 37 cases. The success rate of the operation was 89.19%. Stones recurred in 9 patients during follow-up, for a recurrence rate of 24.32%.ConclusionRetroperitoneoscopic pyeloplasty with simultaneous pyelolithotomy using a flexible cystoscope in patients with UPJO complicated with kidney stones is safe, effective and worthy of promotion.
Multigenerational metabolic disruption: Developmental origins and mechanisms of propagation across generations
Authors: Davis Daniel D. , Diaz-Castillo Carlos , Chamorro-Garcia Raquel 
Published: August 2022

It has been long known that the environment plays a critical role in the etiology of disease. However, it is still unclear how the large variety of environmental factors humans are exposed to interact with each other to lead to disease. Metabolic disorders are just one example of human disorders that have been associated with environmental exposures. Obesity and type 2 diabetes have become a health and economic burden worldwide as the number of affected people has tripled in the last 40 years. Animal and human studies have shown a strong association between exposure to environmental chemicals during critical windows of susceptibility such as periconception, prenatal, and early life, whose effect can persist through development and across generations. However, little is known about the mechanisms driving this persistence. Here, we review historical and current knowledge on the effect of exposure to environmental factors during in utero development and discuss mechanisms for these disorders to be propagated across generations.
Phylogenomic Placement of American Southwest-Associated Clinical and Veterinary Isolates Expands Evidence for Distinct Cryptococcus gattii VGVI
Authors: Monroy-Nieto Juan , Bowers Jolene R. , Montfort Parker , Adame Guillermo , Taverna Constanza Giselle , Yaglom Hayley , Sykes Jane E. , Brady Shane , Mochon A. Brian , Meyer Wieland , Komatsu Kenneth , Engelthaler David M. 
Published: August 2022

Whole-genome sequencing has advanced our understanding of the population structure of the pathogenic species complex Cryptococcus gattii, which has allowed for the phylogenomic specification of previously described major molecular type groupings and novel lineages. Recently, isolates collected in Mexico in the 1960s were determined to be genetically distant from other known molecular types and were classified as VGVI. We sequenced four clinical isolates and one veterinary isolate collected in the southwestern United States and Argentina from 2012 to 2021. Phylogenomic analysis groups these genomes with those of the Mexican VGVI isolates, expanding VGVI into a clade and establishing this molecular type as a clinically important population. These findings also potentially expand the known Cryptococcus ecological range with a previously unrecognized endemic area.
Sonochemical Synthesis of Cu@Pt Bimetallic Nanoparticles
Authors: Hansen Henrik E. , Fakhri Daniel Ø. , Seland Frode , Sunde Svein , Burheim Odne S. , Pollet Bruno G. 
Published: August 2022

Reducing the amount of noble metals in catalysts for electrochemical conversion devices is paramount if these devices are to be commercialized. Taking advantage of the high degree of particle property control displayed by the sonochemical method, we set out to synthesize Cu@Pt bimetallic nanocatalysts in an effort to improve the mass activity towards the hydrogen evolution reaction. At least 17 times higher mass activity was found for the carbon supported Cu@Pt bimetallic nanocatalyst (737 mA mg−1, E = −20 mV) compared to carbon supported Pt nanocatalysts prepared with the same ultrasound conditions (44 mA mg−1, E = −20 mV). The synthesis was found to proceed with the sonochemical formation of Cu and Cu2O nanoparticles with the addition of PtCl4 leading to galvanic displacement of the Cu-nanoparticles and the formation of a Pt-shell around the Cu-core.
Medical Prevention of Miscarriage in Women with Ovarian Endometriosis in Reproductive Age
Authors: Гутикова Л.В. , Кухарчик Ю.В. , Павловская М.А. , Шульга А.В. 
Published: August 2022

Под нашим наблюдением находились 63 пациентки с эндометриоидными кистами яичников и наличием репродуктивных планов, которым было проведено двухэтапное комбинированное лечение: лапароскопическая цистэктомия с последующим назначением противорецидивной терапии с учетом иммуногистохимических данных (матриксная металлопротеиназа-9, циклины В1 и D1, иммуногистохимический индекс). У пациентов, получавших агонисты гонадотропин-рилизинг гормона, частота рецидивов составила 16,67%, а 27,8% женщин забеременели, выносили и родили здоровых доношенных детей. Среди пациентов, получавших в послеоперационном периоде дидрогестерон и диеногест, частота рецидивов составила 13,6 и 17,39%, а частота наступления беременности и рождения детей – 63,6 и 73,9% соответственно. Ни у одной из забеременевших женщин не было зарегистрировано угроз прерывания беременности.
We observed 63 patients with endometrioid ovarian cysts and the presence of reproductive plans, who underwent a two-stage combined treatment: laparoscopic cystectomy followed by anti-relapse therapy taking into account immunohistochemical data (matrix metalloproteinase-9, cyclins B1 and D1, immunohistochemical index). In patients treated with gonadotropin-releasing hormone agonists, the relapse rate was 16.67% and 27.8% of women became pregnant, carried and gave birth of healthy full-term babies. Among patients treated in the postoperative period with dydrogesterone and dienogest, the recurrence rate was 13.6 and 17.39%, and the pregnancy and childbirth rates were 63.6 and 73.9%, respectively. None of the pregnant women had a threatened miscarriage.