bluuebunny/crossref_metadata_2025_split huggingface dataset:
The clinical impact of age-specific distribution combination patterns of cytology &amp; high-risk HPV status in the background of cervical intraepithelial neoplasia grade3 or greater
Authors: Sakai Mie , Ohara Tsutomu , Suzuki Haruka , Kadomoto Tatsuki , Inayama Yoshihide , Shitanaka Shimpei , Sumitomo Masahiro , Matsumura Noriomi , Yamanoi Koji 
Published: May 2022

Abstract
Purpose:This is the first study to elucidate the significance of cytology & high-risk Human Papillomavirus (hrHPV) status in different age groups for detection of cervical intraepithelial neoplasia grade 3 (CIN3) and squamous cell carcinoma (SCC).Methods:There were 12 combinations based on cytology & hrHPV status (Cytology; ASC-US, LSIL, ASC-H and HSIL, hrHPV status; HPV16/18 positive (16/18+), hrHPV other than 16/18 positive (others+) and hrHPV negative (hrHPV-)). All patients were divided into 4 groups based on age (18-29, 30-39, 40-49, 50-).1) For CIN3 and SCC (CIN3+) patients (n=84), the distribution of cytology & hrHPV was investigated in each age group.2) For all patients (n=446), the frequencies of CIN3+ for each of 12 combinations were investigated in each age group.Results:1) In the 18-29 group, HSIL & 16/18+ accounted for as high as 54.5% of CIN3+. The proportion of HSIL & 16/18+ decreased markedly with increasing age, and there were no cases in the years 50- group. Instead, ASC-US & others+ gradually increased with age, up to 25%.2) In the 18-29 group, all patients in the HSIL & 16/18+ group were diagnosed as CIN3+. On the contrary, no patients in hrHPV- & HSIL were diagnosed as CIN3+.Such a clear distinction was not observed in any other age group.Conclusion:For CIN3+, the distribution patterns of cytology & hrHPV status combination significantly varied among the different age groups. Accordingly, the clinical impact of the combination of cytology findings & hrHPV status can vary among age groups.
LncRNA MILIP links YBX1 to translational activation of Snai1 and promotes metastasis in clear cell renal cell carcinoma
Authors: Wang Yanliang , Feng Yu Chen , Gan Yujin , Teng Liu , Wang Li , La Ting , Wang Peilin , Gu Yue , Yan Lei , Li Na , Zhang Lina , Wang Limeng , Thorne Rick F. , Zhang Xu Dong , Cao Huixia , Shao Feng-Min 
Published: May 2022

Abstract
Background: Distant metastasis is the major cause of clear cell renal cell carcinoma (ccRCC)-associated mortality. However, the molecular mechanisms involved in ccRCC metastasis remain to be fully understood. With the increasing appreciation of the role of long noncoding RNAs (lncRNAs) in cancer development, progression, and treatment resistance, the list of aberrantly expressed lncRNAs that contribute to ccRCC pathogenesis is also expanding rapidly.Methods: Bioinformatics analysis was carried out to interrogate publicly available ccRCC datasets. In situ hybridization and qRT-PCR assays were used to test lncRNA expression in human ccRCC tissues and cell lines, respectively. Chromatin immunoprecipitation and luciferase reporter assays were used to examine transcriptional regulation of gene expression. Wound healing as well as transwell migration and invasion assays were employed to monitor ccRCC cell motility and invasion in vitro. Mouse models of ccRCC metastasis was conducted to model metastasis in vivo. RNA pulldown and RNA immunoprecipitation were performed to test RNA-protein associations, and domain-specific chromatin isolation by RNA purification was used to test the RNA-RNA interaction. SiRNA and shRNA were conducted to knockdown gene expression. Results: MILIP expression was upregulated in metastatic compared with primary ccRCC cells. The increased MILIP expression in metastatic ccRCC cells is driven by transcriptional activation mediated by the transcription factor AP-2 gamma (TFAP2C). Knockdown of MILIP diminished the potential of ccRCC cell migration and invasion and reduced formation of metastatic lesions in vivo. This was associated with reductions in the expression of epithelial-to-mesenchymal transition (EMT) hallmark gene signatures. MILIP formed an RNA-RNA duplex with snail family transcriptional repressor 1(Snai1) mRNA and also bound to Y-box binding protein 1 (YBX1). This promoted the association of YBX1 and Snai1 leading to increased translation of Snai1 mRNA. Snai1 in turn played an important part in MILIP-mediated promotion of ccRCC metastasis. Conclusions: The TFAP2C-responsive lncRNA MILIP is upregulated in metastatic ccRCC and plays an important role in driving ccRCC cell metastasis. Thus, MILIP targeting is potentially a novel avenue for ccRCC treatment.
Prediction Model for Irreversible Intestinal Ischemia in Strangulated Bowel Obstruction
Authors: Kobayashi Toshimichi , Chiba Naokazu , Koganezawa Itsuki , Nakagawa Masashi , Yokozuka Kei , Ochiai Shigeto , Gunji Takahiro , Sano Toru , Tomita Koichi , Tabuchi Satoshi , Hidaka Eiji , Kawachi Shigeyuki 
Published: May 2022

Abstract
Background: Preoperatively diagnosing irreversible intestinal ischemia in patients with strangulated bowel obstruction is difficult. Therefore, this study aimed to establish a prediction model for irreversible intestinal ischemia in strangulated bowel obstruction.Methods: We included 83 patients who underwent emergency surgery for strangulated bowel obstruction between January 2014 and March 2022. The predictors of irreversible intestinal ischemia in strangulated bowel obstruction were identified using logistic regression analysis, and a prediction model for irreversible intestinal ischemia in strangulated bowel obstruction was established using the regression coefficients. Receiver operating characteristic analysis and 5-fold cross-validation was used to assess the model.Results: The prediction model (range, 0−4) was established using a white blood cell count of ≥ 12000/µL and the computed tomography value of peritoneal fluid that was ≥ 20 Hounsfield units. The areas of the receiver operating characteristic curve of the new prediction model were 0.814 and 0.807 after 5-fold cross-validation. A score of ≥ 2 was strongly suggestive of irreversible intestinal ischemia in strangulated bowel obstruction and necessitated bowel resection (odds ratio = 15.938). The bowel resection rates for the prediction scores of 0, 2, and 4 were 15.2%, 66.7%, and 85.0%, respectively.Conclusions: Our model may help predict irreversible intestinal ischemia that necessitates bowel resection for strangulated bowel obstruction cases and thus enable surgeons to recognize the severity of the situation and prepare for deterioration of patients with progression of intestinal ischemia.
Clinical observation of hypofibrinogen induced by the treatment of tocilizumab in rheumatic disease
Authors: An Qi , Yuan DaWei , Huang Jing , Ju BoMiao , Xu QianYun , Wang Jing , Luo Jing , Zhang Jing , Wang YanHua , Hu Nan , Pan Ying , Wang Pei , LV XiaoHong , Pu Dan , He Lan 
Published: May 2022

Abstract
Background: By analyzing the changes of fibrinogen levels in plasma during tocilizumab-treated patients with rheumatic diseases, and the aim was to clarify the incidence and possible influencing factors of hypofibrinogenemia.Methods: These tocilizumab-treated patients with rheumatic diseases in the First Affiliated Hospital of Xi 'an Jiaotong University from January 2014 to October 2021 were included, to observe the reduced level of fibrinogen (FIB) in plasma at different time points in different diseases, explore the correlation between FIB and other acute phase proteins including Erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), high-sensitivity C-reactive protein (hsCRP) in rheumatoid arthritis (RA) patients, and explore the risk factors for FIB reduction among RA patients treated with tocilizumab(TCZ) by COX regression.Results: A total of 94 tocilizumab-treated patients were included in this study. The median frequency of TCZ treatment was 6 (5-7) times, and 32 patients (34.0%) suffered FIB reduction during TCZ treatment. There were 8 males and 24 females in the FIB-reduced group, with a median age of 32 (25-51) years. Hypofibrinogen is most common in patients with Takayasu arteritis (TA) and Rheumatoid Arthritis (RA) treated with TCZ, with an incidence of 63.6% and 28.1%, respectively. In RA patients treated with TCZ, FIB at baseline was positively correlated with acute phase protein ESR (r=0.594, p<0.001), CRP (r=0.593, p<0.001), hsCRP (r=0.564, p<0.001) and C3 (r=0.462, p<0.05). There was a positive correlation between FIB and CRP after 3 months of TCZ treatment (r=0.811, p<0.05), and after 6 months of TCZ treatment (r=0.941, p<0.05). Further COX regression analysis did not find any factors associated with FIB reduction to reach statistical significance.Conclusion: The incidence of FIB reduction was high after TCZ treatment. FIB was correlated with acute phase proteins before and during treatment.
Laparoscopic Roux-en-Y Feeding Jejunostomy as a long term solution for severe feeding problems in children
Authors: Braak H. , Gorter R. R. , Wijk M. P. , Jong J. R. 
Published: May 2022

Abstract
PurposeEnteral feeding is a common problem in children with gastric emptying disorders. Traditional feeding methods in these patients often show a high rate of complications and maintenance issues. Laparoscopic Roux-en-Y feeding jejunostomy (LRFJ) has been described in a few patients as a minimal invasive option for enteral access in these children. The aim of this study is to evaluate the outcomes of the LRFJ procedure in our tertiary referral center.MethodsWe conducted a retrospective case-series including all patients, aged 0-18 years old, that underwent a LFRJ procedure between August 2011 and December 2020 for the indication of oral feeding intolerance due to delayed gastric emptying. Outcomes evaluated were complications (short and long term) and parenteral satisfaction.ResultsIn total 12 children were identified that underwent LRFJ for the indication of oral feeding intolerance due to delayed gastric emptying. A total of 16 complications were noted in 8/12 patients (67%). Severity classified by Clavien-Dindo were grade I (n=13), grade II (n=1) and grade IIIB (n=2). In 11/12 patients, parents were satisfied with the results. ConclusionsAlthough minor complications after LRFJ are common in our patients, this technique is a safe solution in patients with gastric emptying disorders leading to a definitive method of enteral feeding and high parenteral satisfaction.
Spatiotemporal distribution of high-fluoride in groundwater and its health risk assessment in children in a new dental fluorosis field
Authors: Karabulut Benan Yazici , Derin Perihan , Yetis Ayşegul Demir , Yesilnacar Mehmet Irfan 
Published: May 2022

Abstract
This study focused on identifying drinking water sources of fluoride concentrations in arid and semi-arid areas of Sanliurfa province in southeastern Turkey. Fluorosis risk assessment and characterization were also studied. The fluoride levels of the study area were mapped using Geographical Information Systems (GIS). Water samples were collected and analyzed for each season from a total of fifty-three points. According to the results, many points had contaminated water sources. The average annual concentration levels in water resources in the study area were 0.26–3.62 mg/L. The results of spatial variation show that the Sarim and Karatas region has the highest concentration of fluoride distribution. The highest fluoride health risk in this region was observed in children, followed by teenagers and adults. Oral exposure rate was much higher than dermal exposure. In this region in particular, serious defects and staining have been detected in the teeth of school-age children with fourth- and fifth-degree fluorosis. Removing fluoride from drinking water with appropriate treatment methods such as electrocoagulation and adsorption will reduce the health risks associated with groundwater fluoride exposure.
Liquefaction Potential based on ground investigation data and natural frequency
Authors: Putra Rusnardi Rahmat , Kiyono Junji , Agung Darma 
Published: May 2022

Abstract
An earthquake can trigger other disasters. Liquefaction is one of them and it occurs due to the reduction or loss of effective stress in soil due to cyclic loads. Liquefaction may cause the ground to act as a flowing liquid and become harmful to the building above it. Damage to a building’s structure can occur because of the strong surface waves from soil thrust and flow. Thus, an evaluation of the liquefaction potential is needed to prevent building damage. This study aims to determine the vulnerability of Padang City to liquefaction disasters and proposes a liquefaction potential based on the natural frequency at the surface. The study was conducted by analysing soil investigation data (54 CPT and SPT), using earthquake acceleration to obtain the value of the safety factor (SF), and single microtremor observations, to determine the natural frequency of the soil at these points. The results show that the form of liquefaction potential in Padang City varies depending on the depth of the reviewed soil. At a depth of 1 m, almost the entire research area has liquefaction potential. The coastal area has a very high level of vulnerability. In general, the liquefaction potential decreases with increasing depth. The liquefaction potential and the natural frequency of the soil (f0) are related; soil with an f0 value of more than 0.432 Hz does not have a liquefaction potential.
Description and genome analysis of Actobacterium tashihtau gen. nov., sp. nov., a noval Denitrifying and Carbon-fixing bacterium isolated from marine sediment
Authors: Yu Zi-Liang , Jia Ya-Wei , Du Yi-Heng , Du Zong-Jun , Mu Da-Shuai 
Published: May 2022

Abstract
A Gram-stain-negative, non-motile, aerobic, red colony, mesophilic, and slightly halotolerant bacteria, designated strain D16T, was separated from intertidal surface sediments from Xiaoshi Island, Weihai, China. The strain was able to grow at 10-43°C, pH 5.5-9.5 and in 0-5 % (w/v) NaCl. Optimum growth conditions of the strain were at pH 7.0, 30-33 °C and 1 % (w/v) NaCl. The predominant respiratory quinone, major fatty acids and polar lipids of the strain were (MK-6), (C15:0 iso, 32.0 %, C16:0, 10.5 %) and (DPG、PE、AGL、 GL、 AL、PGS), respectively. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain D16T was the closest to genera Eudoraea, Poritiphilus, Muriicola, Robiginitalea and Zeaxanthinibacter, sharing 92-93 % 16S rRNA gene sequence similarity with the representative of these genera. The draft genome of strain D16T composed 3,225,638 bp, and the G+C content was 42.8 mol %. The ANI, dDDH, AAI values of strain D16T compared to similar genera ranged from 67.3 to 70.2 %, 16.2 to 19.1 % and 68.3 to 70.1 %, respectively, below the threshold for recommended species division of ANI 95–96%, dDDH 70% and AAI 95%. Furthermore, POCP values between strain D16T and similar genera were less than 50.0%, consistent with the description of a new genus. Based on the results of phylogenetic, phenotypic, genotypic, chemotaxonomic analyses and comparative analysis of metabolic pathway, environmental distribution and characterization between in strain D16T and other relative genera of family Flavobacteriaceae, it is concluded that strain D16T represents a novel species of a noval genus of the family Flavobacteriaceae, for which the name Actobacterium tashihtau gen.nov., sp. is proposed. The type strain is D16T (=MCCC 1H00463T= KCTC 82746T).
Multiple and multiliteracies: dilemmas in New Zealand secondary science classrooms
Authors: Shahzadi Nosheen 
Published: May 2022

Abstract
Changes in literacy theory and practices in relation to technological change necessitate shifts in contemporary pedagogical practices. In the New Zealand context, little is known about literacy in terms of multiliteracies pedagogical practices in secondary science classrooms. This study aims to unearth the realities of dilemmas operating in literacy contexts. It is argued that evidence for successful enactment of multiliteracies are scarce. More research work and scholarly debates are needed to clarify and align mismatches between theory and practice. Pedagogical moves as hybrid literacies are suggested carrying potential for students in becoming multi-literate.
Determination of Microsporidia Infection in Larvae and Adult Anopheles Mosquitoes (Diptera: Culicidae) Northwest Ethiopia
Authors: Minwuyelet Awoke , Aschale Yibeltal , Atnafu Getnet 
Published: May 2022

Abstract
Background: Microsporidia are a diverse group of obligate intracellular, spore forming parasites that infect all phyla of invertebrates. Microsporidia are known to infect a wide variety of animals including mosquitoes. A few studies showed that microsporidia infection of mosquitoes is associated with larval mortality or reduced adult fecundity and lifespan. The aim of this study is to determine microsporidia infection in field collected Anopheles larvae and reared adults.Methods: Anopheles larvae were collected from the field and analyzed from February to April 2022. The 4th stage larvae, after determining the species identity, they were dissected to extract their midgut while the early stage larvae were transported to entomological laboratory for reareing the adult in the insectary room. Similarly, after determining the species identity they were dissected to extract their midgut. Then two thin smears for each dissected larvae and/ adult were made. Fixed preparations were stained with Giemsa and modified Ziehl-Neelsen staining techniques to detect the microsporidia. Descriptive statistics and independent t-test were used to analyze the data.Results: Anopheles gambiae complex was identified as the most predominant species from field collected larvae and insectary reared adults. From the total 258 larvae and 258 adult An.gambiae complexes tissue smears examined, microsporidia were detected in 2.7% [7/258, (95%, CI: 0.8-5)] and 1.2% [3/258, (95%, CI: 0.01-2.7)] of larvae and adult respectively. The mean density of microsporidia in larvae and adults were 64.9 (± 23.4 SD) and 36 (± 8.5SD) respectively. The difference of microsporidia density between infected larvae and adults was statistically significant (F=1.77, P= 0.02). Conclusion: Significant level of microsporidia infection was detected using light microscopy. Further microsporidia identification down to the genus and species level needs to determine its ultrastructure characteristics and the comparative analysis of small subunit rRNA sequence data.
Public Health Communication: Consistency, Accuracy, and Community Engagement during the COVID-19 Pandemic
Authors: Lowe Maya , Harmon Shawn , Kholina Ksenia , Parker Rachel , Graham Janice 
Published: May 2022

Abstract
Objectives
Communication is central to the implementation and efficacy of public health measures. This paper explores public health messaging in Canada during the COVID-19 pandemic, assessing its potential to encourage or undermine public trust and adherence.
Methods
This study analyzed data from two primary sources. The first is government press briefings, associated press releases, and public health directives from January 2020 to October 2021 in Nova Scotia, Ontario, and Alberta. The second data source is 34 semi-structured key-informant interviews with public health actors across Canada. A directed qualitative content analysis approach was employed to analyze press briefing transcripts. Team-based coding and thematic analysis were conducted to analyze interview transcripts.
Results
Three main themes emerged from the data: inconsistency, lack of clarity, and need for engaged communication practice. Ambiguous and inconsistent language practices across and within jurisdictions were common. Clear language that combines scientific evidence with an appeal to social and emotional factors was lacking, specifically in relation to nuanced matters. Communication practices revealed a disconnect between local communities and jurisdictional communicators who often lacked sensitivity and understanding of local concerns and values.
Conclusion
Effective communication must be consistent, clear, and community-driven. Increased federal leadership surrounding public health communication, further jurisdictional collaboration, improved communication training, established engagement infrastructure, and increased diversity of decision-makers and communicators are suggested to improve the effectiveness of communication practices by instilling public trust and thus adherence with public health measures.
Can D&amp;O insurance improve corporate ESG performance?
Authors: Zhao Jing , Xu Hanyou 
Published: May 2022

Abstract
D&O insurance has gained mature practice in developed countries. However, as of November 2021, no more than 15% of listed companies in China have purchased D&O insurance. Combining China’s unique institutional culture and economic background, we examine the impact of D&O insurance on corporate ESG performance. The results show that D&O insurance significantly improves corporate ESG performance. Meanwhile, this positive relationship is affected by economic policy uncertainty and industry competition. Additional analysis suggests that the contribution of D&O insurance to ESG performance is achieved by improving independent director function-performing effectiveness and increasing corporate risk-taking. The findings of this paper have policy implications for a better understanding of the governance role of D&O insurance, encouraging companies to improve corporate ESG performance.
JIPipe: Visual batch processing for ImageJ
Authors: Figge Marc , Gerst Ruman , Cseresnyes Zoltan 
Published: May 2022

Abstract
The continuous development of new microscopy techniques requires the parallel evolution of image analysis workflows. ImageJ provides a high level of accessibility to bioimage processing, which is still impeded by the necessity of developing scripts to achieve reproducibility, and to comply to the FAIR principles. We provide a visual language termed JIPipe that allows the construction of an ImageJ workflow purely by designing a flowchart. We already included over 1000 functions from ImageJ and its plugins. In return, ImageJ is extended with custom-designed algorithms, thus forming a symbiotic relationship with JIPipe. Our software includes a fully reproduceable and standardized project format, zero-cost scalability of pipelines, as well as automated data saving into an open format. JIPipe was already utilized to solve numerous demanding image analysis tasks, showcasing its wide applicability and adaptability. JIPipe contributes towards making bioimage analysis more accessible, thereby fostering collaborations between experimentalists and computer scientists.
The implications of telecommuting on work-life balance: Effects on work engagement and work exhaustion
Authors: Bhat Zahid Hussain , Yousuf Uqba , Saba Nuzhat 
Published: May 2022

Abstract
The Covid-19 pandemic has forced many workers to telecommute, and the public sector is no exception to it. Working from home has resulted in a work overload in personal places. This has eroded the line between personal and professional life, increasing the risk of conflict. The impact of remote working on the balance between life and work is unknown. This article reviews the existing research on work-life balance. It further examines the impact of remote work and telecommuting on work-life balance. The study found that work-life interaction and workplace factors influence employee engagement and exhaustion. Work-related expectations, such as emotional and time demands, must be addressed to decrease work-family conflict and create a good work-life balance. Human resource managers in the sector should develop work-life balance policies and practices. This review adds to the body of knowledge by establishing the importance of work-family conflict and work-life balance for remote employees. This review produced two scholarly contributions to work-life balance. Beginning with an overview of existing research topics and organized work-life balance concepts, second, it identified crucial study areas to understand distant work-life balance better. Implications for practice and future research directions are discussed as well.