bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caveolin‐1 influences mitochondrial plasticity and function in hepatic stellate cell activation
Authors: Ilha Mariana , Meira Martins Leo A. , da Silveira Moraes Ketlen , Dias Camila K. , Thomé Marcos P. , Petry Fernanda , Rohden Francieli , Borojevic Radovan , Trindade Vera M. T. , Klamt Fábio , Barbé‐Tuana Florência , Lenz Guido , Guma Fátima C. R. 
Published: August 2022

AbstractCaveolin‐1 (Cav‐1) is an integral membrane protein present in all organelles, responsible for regulating and integrating multiple signals as a platform. Mitochondria are extremely adaptable to external cues in chronic liver diseases, and expression of Cav‐1 may affect mitochondrial flexibility in hepatic stellate cells (HSCs) activation. We previously demonstrated that exogenous expression of Cav‐1 was sufficient to increase some classical markers of activation in HSCs. Here, we aimed to evaluate the influence of exogenous expression and knockdown of Cav‐1 on regulating the mitochondrial plasticity, metabolism, endoplasmic reticulum (ER)‐mitochondria distance, and lysosomal activity in HSCs. To characterize the mitochondrial, lysosomal morphology, and ER‐mitochondria distance, we perform transmission electron microscope analysis. We accessed mitochondria and lysosomal networks and functions through a confocal microscope and flow cytometry. The expression of mitochondrial machinery fusion/fission genes was examined by real‐time polymerase chain reaction. Total and mitochondrial cholesterol content was measured using Amplex Red. To define energy metabolism, we used the Oroboros system in the cells. We report that GRX cells with exogenous expression or knockdown of Cav‐1 changed mitochondrial morphometric parameters, OXPHOS metabolism, ER‐mitochondria distance, lysosomal activity, and may change the activation state of HSC. This study highlights that Cav‐1 may modulate mitochondrial function and structural reorganization in HSC activation, being a potential candidate marker for chronic liver diseases and a molecular target for therapeutic intervention.
Hair we grow again: Upward mobility, career compromise, and natural hair bias in the workplace
Authors: Summers LaTonya M. , Davis Tonya , Kosovac Bilal 
Published: August 2022

AbstractNatural hair bias, the targeting of people based on their hair or hairstyle, is an emerging but recurring barrier Black women face in the workplace. The aim of this qualitative study was to investigate whether early‐career Black women perceived that their ability to advance occupationally was compromised by wearing natural hair. Semi‐structured interviews were conducted with nine Black women with less than 2 years of work experience in various professions. Using interpretative phenomenological analysis (IPA), the researchers extracted five main themes: appearance and professionalism, double standards, natural hair journey, identity, and invisible rules of engagement at work.
Fast Cysteine Bioconjugation Chemistry
Authors: Chen Fa‐Jie , Gao Jianmin 
Published: August 2022

AbstractCysteine bioconjugation serves as a powerful tool in biological research and has been widely used for chemical modification of proteins, constructing antibody‐drug conjugates, and enabling cell imaging studies. Cysteine conjugation reactions with fast kinetics and exquisite selectivity have been under heavy pursuit as they would allow clean protein modification with just stoichiometric amounts of reagents, which minimizes side reactions, simplifies purification and broadens functional group tolerance. In this concept, we summarize the recent advances in fast cysteine bioconjugation, and discuss the mechanism and chemical principles that underlie the high efficiencies of the newly developed cysteine reactive reagents.
Hydroxy‐Functionalized Hypercrosslinked Polymers (HCPs) as Dual Phase Radioactive Iodine Scavengers: Synergy of Porosity and Functionality
Authors: Samanta Partha , Dutta Subhajit , Let Sumanta , Sen Arunabha , Shirolkar Mandar M. , Ghosh Sujit K. 
Published: August 2022

AbstractLarge‐scale nuclear power plant production of iodine radionuclides (129I, 131I) pose huge threat in the events of nuclear disaster. Effective removal of radioiodine from nuclear waste is one of the most critical challenge because of the drawbacks of state‐of‐the‐art adsorbents such as high cost, low uptake capacity and non‐recyclability. Herein, two hydroxy‐functionalized (‐OH) hypercrosslinked polymers (HCPs), namely HCP‐91 and HCP‐92, have been synthesized and employed towards capture of iodine. High chemical stability along with synergistic harmony of high porosity and functionality of these materials makes them suitable candidates for capture of iodine from both vapor phase and water medium. Moreover, both the HCPs showed superior iodine removal performance from water in terms of fast kinetics and high removal efficiency (2.9 g g−1 and 2.49 g g−1 for HCP‐91 and HCP‐92 respectively). The role of functionality (‐OH groups) and porosity has been established with the help of HCP‐91, HCP‐92 and non‐functionalized biphenyl HCP for the efficient capture of I3− ions from water. In addition, both HCPs exhibited excellent selectivity and recyclability towards triiodide ions, rendering the potential of these materials towards real‐time applications. Lastly, Density functional theoretical studies revealed key insights and corroborate well with the experimental findings.
Longer board tenure and audit committee tenure. How do they impact environmental performance? A European study
Authors: Paolone Francesco , Pozzoli Matteo , Cucari Nicola , Bianco Rosario 
Published: August 2022

AbstractWe analyze the impact of board of directors (BoD) tenure and audit committee (AC) tenure on environmental performance (EP) to test whether BoD and AC members' rotation generates higher environmental performance. Data were collected from the Refinitiv Eikon database, structuring a sample of European Union (EU) listed companies belonging to the “old” country members in the period from 2018 to 2020. We found that longer board tenure and longer audit committee tenure each enhance environmental performance, demonstrating that firms could view the tenure rate as a proxy of the quality of the board monitoring. On the contrary, the interaction term (BoD and AC tenure) is negatively related to environmental performance, showing that when two boards have a low rotation, companies may achieve lower environmental performance. These findings help understand the dynamic and complex nature of tenure in corporate governance mechanisms.
3D‐printed autoclavable plant holders to facilitate large‐scale protein production in plants
Authors: Chuang Ling , Enders Anton , Offermann Sascha , Bahnemann Janina , Franke Jakob 
Published: August 2022

AbstractThe Australian tobacco plant Nicotiana benthamiana is becoming increasingly popular as a platform for protein production and metabolic engineering. In this system, gene expression is achieved transiently by infiltrating N. benthamiana plants with suspensions of Agrobacterium tumefaciens carrying vectors with the target genes. To infiltrate larger numbers of plants, vacuum infiltration is the most efficient approach known, which is already used on industrial scale. Current laboratory‐scale solutions for vacuum infiltration, however, either require expensive custom‐tailored equipment or produce large amounts of biologically contaminated waste. To overcome these problems and lower the burden to establish vacuum infiltration in new laboratories, we present here 3D‐printed plant holders for vacuum infiltration. We demonstrate that our plant holders are simple to use and enable a throughput of around 40 plants per hour. In addition, our 3D‐printed plant holders are made from autoclavable material, which tolerate at least 12 autoclave cycles, helping to limit the production of contaminated waste and thus contributing to increased sustainability in research. In conclusion, our plant holders provide a simple, robust, safe and transparent platform for laboratory‐scale vacuum infiltration that can be readily adopted by new laboratories interested in protein and metabolite production in Nicotiana benthamiana.Practical applicationTransient expression in Nicotiana benthamiana provides a popular and rapid system for producing proteins in a plant host. To infiltrate larger numbers of plants (typically >20), vacuum infiltration is the method of choice. However, no system has been described so far which is robust to use and can be used without expensive and complex equipment. Our autoclavable 3D‐printed plant holders presented here will greatly reduce the efforts required to adopt the vacuum infiltration technique in new laboratories. They are easy to use and can be autoclaved at least 12 times, which contributes to waste reduction and sustainability in research laboratories. We anticipate that the 3D printing design provided here will drastically lower the bar for new groups to employ vacuum infiltration for producing proteins and metabolites in Nicotiana benthamiana.
Late erosion pulse triggered by rapid melt in the cold‐based interior of the last Fennoscandian Ice Sheet, an example from Rogen
Authors: van Boeckel Mikis , van Boeckel Tayo , Hall Adrian M. 
Published: August 2022

AbstractIce sheet interiors are conventionally regarded as non‐erosive. Yet subglacial conditions may be transformed during deglaciation by the arrival of large volumes of meltwater at the ice sheet bed. The development of a dynamic meltwater drainage system and the onset of basal sliding have potential to increase erosion rates in bedrock and sediment. Here, we examine the impact of late deglacial thawing on the Rogen plateau, located near the former ice divide of the Fennoscandian Ice Sheet. We provide new maps of glacial and glacifluvial landforms which we combine with existing data on Quaternary sediments and landforms. Cross‐cutting and overlapping relations allow for an event sequence to be established of the deglaciation period. In the Early Holocene (< 11 ka), an ice lobe onset zone developed at the Rogen plateau. In places where meltwater reached the bed and where pressures rose to overpressure, it caused fracture dilation in horizontally bedded sandstones and rock brecciation. The onset of sliding and application of drag resulted in the mobilization of bedrock sheets. The establishment of meltwater corridors led to fluidization of sediments at the bed, dissection and modification of ribbed moraines and formation of murtoos and hummock corridors. During final stagnation of the ice sheet, meltwater drained through channels forming axial eskers. Bedrock erosion during deglaciation reached depths up to 4 m, and in conjunction with some recycling of till, generated
317 km2 of boulder cover. The average erosion depths by removal and reworking of sediment are
0.9–1.1 m across areas below 900 m elevation. This study shows that when the cold‐based interiors of ice sheets become briefly activated by large subglacial meltwater delivery late in deglaciation, there can be significant reworking and erosion of rock and sediment.
The association between ballroom dance training and empathic concern: Behavioral and brain evidence
Authors: Wu Xiao , Lu Xuejing , Zhang Huijuan , Wang Xiao , Kong Yazhuo , Hu Li 
Published: August 2022

AbstractDance is unique in that it is a sport and an art simultaneously. Beyond improving sensorimotor functions, dance training could benefit high‐level emotional and cognitive functions. Duo dances also confer the possibility for dancers to develop the abilities to recognize, understand, and share the thoughts and feelings of their dance partners during the long‐term dance training. To test this possibility, we collected high‐resolution structural and resting‐state functional magnetic resonance imaging (MRI) data from 43 expert‐level ballroom dancers (a model of long‐term exposure to duo dance training) and 40 age‐matched and sex‐matched nondancers, and measured their empathic ability using a self‐report trait empathy scale. We found that ballroom dancers showed higher scores of empathic concern (EC) than controls. The EC scores were positively correlated with years with dance partners but negatively correlated with the number of dance partners for ballroom dancers. These behavioral results were supported by the structural and functional MRI data. Structurally, we observed that the gray matter volumes in the subgenual anterior cingulate cortex (ACC) and EC scores were positively correlated. Functionally, the connectivity between ACC and occipital gyrus was positively correlated with both EC scores and years with dance partners. In addition, the relationship between years with dance partners and EC scores was indirect‐only mediated by the ACC‐occipital gyrus functional connectivity. Therefore, our findings provided solid evidence for the close link between long‐term ballroom dance training and empathy, which deepens our understanding of the neural mechanisms underlying this phenomenon.
Analysis ofAspergillus versicolorexudate composition
Authors: Wang Haining , Yang Xiaohe , Wei Songhong 
Published: August 2022

AbstractAspergillus versicolor, a widely distributed fungus, is associated with pollution and carcinogenic hazards. This study aimed to examine the functions of theA.versicolorexudate and laid a scientific foundation for improving our understanding, utilization, and control ofA.versicolor. TheA.versicolorexudate proteome, ion content, and amino acid components were determined using label‐free quantitation, atomic absorption spectrophotometry, and high‐performance liquid chromatography, respectively. In total, 502 proteins were identified in theA.versicolorexudate. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and cluster of orthologous group analyses were used to annotate the functional classification and pathways of the aligned proteins. Proteins identified in the exudate were mainly enriched in carbohydrate metabolic process, translation, oxidoreductase activity, oxidoreductase activity, hydrolase activity, cell wall‐related processes, catalytic activity, and unknown functions. The exudate comprised Na, K, Ca, Fe, and Mg cations. Among the 17 types of amino acids detected in the exudate, 7 were essential and 10 were nonessential. The exudate may be involved in the vital processes ofA.versicolor. Additionally, the exudate may play an important role in the growth, development, reproduction, homeostasis, nutrient supply for regrowth, and virulence ofA.versicolor.
Machine learning models for predicting soil particle size fractions from routine soil analyses in Quebec
Authors: Martinelli Gaëtan , Gasser Marc‐Olivier 
Published: August 2022

AbstractSoil texture and particle size distribution are important soil properties for understanding and interpreting the multiple processes and interactions in soil agrosystems. However, soil particle size analysis is rarely included in routine soil laboratory analyses due to cost but can be derived from other soil physico‐chemical properties and from more powerful regressions using machine learning techniques. The performance of multiple linear regression and four machine learning algorithms (MLAs)—K‐nearest neighbor (KNN), Random Forest, extra‐gradient boosting (XGBoost), and multilayer neural network (NeuralNetwork)—was compared to predict particle size fractions of clay, sand, and silt using routine analyses of soil pH, cation exchange capacity, Mehlich‐3 extracted elements, and density of dried sieved soil from 8,364 soil samples distributed across Quebec. Particle size fractions were predicted as compositional data using isometric log ratio transformation. The XGBoost model performed best, with RMSE values of 7, 10, and 12% and R2 values of .77, .57, and .73 for the prediction of clay, silt, and sand fractions, respectively. Feature importance classification varied from one model to another, but the best predictors remained the same regardless of the model used. Sieved soil density measured in the laboratory and Mehlich‐3 Mg, K, Fe, and Zn ranked as better predictors of particle size fractions. Predicting soil particle sizes using routine soil physico‐chemical properties and MLAs appears an option for incomplete legacy datasets and a promising economic alternative to current methods carried out in commercial laboratories.
Long‐Term Outcomes After Lobectomy for Patients with High‐Risk Papillary Thyroid Carcinoma
Authors: Sugitani Iwao , Kazusaka Hiroko , Ebina Aya , Shimbashi Wataru , Toda Kazuhisa , Takeuchi Kengo 
Published: August 2022

AbstractBackgroundGuidelines universally recommend total thyroidectomy for high‐risk papillary thyroid carcinoma (PTC). However, in Japan, thyroid‐conserving surgery had been widely applied for such patients until recently. We investigated long‐term outcomes for this strategy.MethodsA prospectively recorded database was retrospectively analyzed for 368 patients who had undergone curative surgery for high‐risk PTC without distant metastasis between 1993 and 2013. High‐risk PTC was defined for tumors showing tumor size > 4 cm, extrathyroidal extension, or large nodal metastasis ≥ 3 cm.ResultsMedian age was 59 years and 243 patients were female. Mean duration of follow‐up was 12.7 years. Lobectomy was conducted for 207 patients (LT group) and total or near‐total thyroidectomy for 161 patients (TT group). The frequency of massive extrathyroidal invasion and large nodal metastasis was lower in the LT group than in the TT group. After propensity score matching, no significant differences were seen between groups for overall survival, cause‐specific survival or distant recurrence‐free survival. In the overall cohort, multivariate analysis identified age ≥ 55 years, large nodal metastasis, tumor size > 4 cm and massive extrathyroidal invasion as significantly associated with cause‐specific survival, whereas extent of thyroidectomy was not.ConclusionsFor patients with high‐risk PTC without distant metastasis, curative surgery with lobectomy showed almost identical oncological outcomes compared to total thyroidectomy. The benefits of total thyroidectomy for high‐risk PTC should be reevaluated in the future prospective studies.
Fetal magnetic resonance imaging of lumbar spine development in vivo: a retrospective study
Authors: Yin Xing , Zhao Xin , Lu Lin , Zhang Liying , Xing Qingna , Yuan Rui , Niu Zhijun , Zhang Linlin 
Published: August 2022

Abstract
Objective
The aim of this study is to describe MR imaging appearances of the fetal lumbar spine in vivo at different gestational ages (GAs).

Methods
This retrospective study was approved by the Third Affiliated Hospital of Zhengzhou University. We collected MR images and clinical data of 93 fetuses in our hospital. All the MR images were obtained by 3-T MR. All had the mid-sagittal plane of steady state free precession sequence (Trufi) of the lumbar spine, which could show the lumbar vertebra and conus medullaris (CM). Regression analysis was made between GA and heights of lumbar vertebral body ossification center (LVBOC), lengths of LVBOC, and heights of intervertebral gap (IVG).

Results
There were good linear correlations between the heights of LVBOC and GA (P < 0.001), lengths of LVBOC and GA (P < 0.001), and heights of IVG and GA (P < 0.001).

Conclusion
We showed the different development of each LVBOC and IVG which caused the difference of the shape of LVBOC and IVG.
Remote ischaemic conditioning: defining critical criteria for success—report from the 11th Hatter Cardiovascular Workshop
Authors: Bell R. M. , Basalay M. , Bøtker H. E. , Beikoghli Kalkhoran S. , Carr R. D. , Cunningham J. , Davidson S. M. , England T. J. , Giesz S. , Ghosh A. K. , Golforoush P. , Gourine A. V. , Hausenloy D. J. , Heusch G. , Ibanez B. , Kleinbongard P. , Lecour S. , Lukhna K. , Ntsekhe M. , Ovize M. , Salama A. D. , Vilahur G. , Walker J. M. , Yellon D. M. 
Published: August 2022

AbstractThe Hatter Cardiovascular Institute biennial workshop, originally scheduled for April 2020 but postponed for 2 years due to the Covid pandemic, was organised to debate and discuss the future of Remote Ischaemic Conditioning (RIC). This evolved from the large multicentre CONDI-2–ERIC–PPCI outcome study which demonstrated no additional benefit when using RIC in the setting of ST-elevation myocardial infarction (STEMI). The workshop discussed how conditioning has led to a significant and fundamental understanding of the mechanisms preventing cell death following ischaemia and reperfusion, and the key target cyto-protective pathways recruited by protective interventions, such as RIC. However, the obvious need to translate this protection to the clinical setting has not materialised largely due to the disconnect between preclinical and clinical studies. Discussion points included how to adapt preclinical animal studies to mirror the patient presenting with an acute myocardial infarction, as well as how to refine patient selection in clinical studies to account for co-morbidities and ongoing therapy. These latter scenarios can modify cytoprotective signalling and need to be taken into account to allow for a more robust outcome when powered appropriately. The workshop also discussed the potential for RIC in other disease settings including ischaemic stroke, cardio-oncology and COVID-19. The workshop, therefore, put forward specific classifications which could help identify so-called responders vs. non-responders in both the preclinical and clinical settings.
Predictors of remission in rheumatoid arthritis patients treated with biologics: a systematic review and meta-analysis
Authors: Khader Yasmin , Beran Azizullah , Ghazaleh Sami , Lee-Smith Wade , Altorok Nezam 
Published: August 2022

AbstractBiologics have emerged as an effective treatment of rheumatoid arthritis (RA). However, there is a significant proportion of patients who fail to respond to biologics. Identifying the predictors that affect the response to biologics remains challenging. A comprehensive literature search of PubMed, Embase, and Web of Science databases was conducted through May 1, 2022. We included all studies that used a multivariate model to assess for the predictors of remission in RA patients treated with biologics. We calculated pooled odds ratios (OR) with 95% confidence intervals (CI) for risk factors reported in ≥ 3 studies using a random-effects model. A total of 16,934 patients with RA who were treated with biologics were included in twenty-one studies. Our study showed that old age (OR 0.98 (0.97, 0.99), P < 0.00001), female gender (OR 0.66 (0.56, 0.77), P < 0.00001), smoking history (OR 0.86 (0.75, 0.99), P 0.04), obesity (OR 0.95 (0.91, 0.99), P 0.02), poor functional status (OR 0.62 (0.48, 1.27), P < 0.00001), high disease activity (OR 0.90 (0.85, 0.96), P 0.0005), and elevated erythrocyte sedimentation rate (OR 0.99 (0.98, 1.00), P 0.009) were poor predictors of remission. On the other hand, positive anti-citrullinated protein antibodies (OR 2.52 (1.53, 4.12), P 0.0003) was associated with high remission rate. Old age, female gender, obesity, smoking history, poor functional status, high disease activity, and elevated ESR at the time of diagnosis have been associated with poor response to biologics. Our findings could help establish a risk stratification model for predicting the remission rate in RA patients receiving biologics.