bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding the pathogenesis of lean non-autoimmune diabetes in an African population with newly diagnosed diabetes
Authors: Kibirige Davis , Sekitoleko Isaac , Lumu William , Jones Angus G. , Hattersley Andrew T. , Smeeth Liam , Nyirenda Moffat J. 
Published: February 2022

Abstract
Aims/hypothesis
Apparent type 2 diabetes is increasingly reported in lean adult individuals in sub-Saharan Africa. However, studies undertaking robust clinical and metabolic characterisation of lean individuals with new-onset type 2 diabetes are limited in this population. This cross-sectional study aimed to perform a detailed clinical and metabolic characterisation of newly diagnosed adult patients with diabetes in Uganda, in order to compare features between lean and non-lean individuals.

Methods
Socio-demographic, clinical, biophysical and metabolic (including oral glucose tolerance test) data were collected on 568 adult patients with newly diagnosed diabetes. Participants were screened for islet autoantibodies to exclude those with autoimmune diabetes. The remaining participants (with type 2 diabetes) were then classified as lean (BMI <25 kg/m2) or non-lean (BMI ≥25 kg/m2), and their socio-demographic, clinical, biophysical and metabolic characteristics were compared.

Results
Thirty-four participants (6.4%) were excluded from analyses because they were positive for pancreatic autoantibodies, and a further 34 participants because they had incomplete data. For the remaining 500 participants, the median (IQR) age, BMI and HbA1c were 48 years (39–58), 27.5 kg/m2 (23.6–31.4) and 90 mmol/mol (61–113) (10.3% [7.7–12.5]), respectively, with a female predominance (approximately 57%). Of the 500 participants, 160 (32%) and 340 (68%) were lean and non-lean, respectively. Compared with non-lean participants, lean participants were mainly male (60.6% vs 35.3%, p<0.001) and had lower visceral adiposity level (5 [4–7] vs 11 [9–13], p<0.001) and features of the metabolic syndrome (uric acid, 246.5 [205.0–290.6] vs 289 [234–347] μmol/l, p<0.001; leptin, 660.9 [174.5–1993.1] vs 3988.0 [1336.0–6595.0] pg/ml, p<0.001). In addition, they displayed markedly reduced markers of beta cell function (oral insulinogenic index 0.8 [0.3–2.5] vs 1.6 [0.6–4.6] pmol/mmol; 120 min serum C-peptide 0.70 [0.33–1.36] vs 1.02 [0.60–1.66] nmol/l, p<0.001).

Conclusions/interpretation
Approximately one-third of participants with incident adult-onset non-autoimmune diabetes had BMI <25 kg/m2. Diabetes in these lean individuals was more common in men, and predominantly associated with reduced pancreatic secretory function rather than insulin resistance. The underlying pathological mechanisms are unclear, but this is likely to have important management implications.

Graphical abstract
Assessing the effectiveness of social network interventions for adults with a diagnosis of mental health problems: a systematic review and narrative synthesis of impact
Authors: Brooks Helen , Devereux-Fitzgerald Angela , Richmond Laura , Bee Penny , Lovell Karina , Caton Neil , Cherry Mary Gemma , Edwards Bethan Mair , Downs James , Bush Laura , Vassilev Ivaylo , Young Bridget , Rogers Anne 
Published: February 2022

Abstract
Background
Social connections have been linked to the genesis and amelioration of mental health problems and thus have potential therapeutic value.

Purpose
To identify the current evidence base, assess risk of bias and synthesise findings on the effectiveness of social network interventions for people with mental health problems.

Methods
Electronic databases (MEDLINE, Embase, PsycINFO, CINAHL, Cochrane Library, Web of Science, Scopus) and grey literature databases were systematically searched from inception to October 2021 using free text syntax combining synonyms for ‘mental health problems’ and ‘social network interventions’. Articles were eligible for inclusion if they reported data from randomised controlled trials on the effectiveness of interventions designed to improve social networks for adults (18+) with mental health problems. Papers were independently reviewed for inclusion with conflicts resolved through consensus. Included papers were quality assessed and data extracted and synthesized narratively. Risk of bias was assessed using the Cochrane Risk of Bias Tool.

Results
Nine studies randomising 2226 participants were included. Four focused on those with a diagnosis of schizophrenia or psychosis, one on major depressive disorder and four included all types of mental health diagnoses. The current evidence base is of unclear quality. However, interventions which focused on supporting social activities appear to hold the most promise for enhancing social networks. Data on cost-effectiveness and research acceptability were limited, but suggest the potential economic feasibility of and acceptability for evaluating these interventions.

Conclusion
There is emerging evidence that social network interventions can be effective in improving social connections for people with mental health problems. However, further evaluations with robust methodological approaches are required to inform evidence-based recommendations for health services.
Circulating microRNA miR-137 as a stable biomarker for methamphetamine abstinence
Authors: Kim Baeksun , Tag Sung Hyun , Kim Yong Sik , Cho Sung Nam , Im Heh-In 
Published: February 2022

Abstract
Objective
Stimulant use instigates abstinence syndrome in humans. miRNAs are a critical component for the pathophysiology of stimulant abstinence. Here we sought to identify a miRNA marker of methamphetamine abstinence in the circulating extracellular vesicles (cEVs).

Methods
miR-137 in the cEVs was quantified by qPCR in thirty-seven patients under methamphetamine abstinence and thirty-five age-matched healthy controls recruited from 2014 to 2016 from the general adult population in a hospital setting, Seoul, South Korea. Diagnostic power was evaluated by area under curve in the receiver-operating characteristics curve and other multiple statistical parameters.

Results
Patients under methamphetamine abstinence exhibited a significant reduction in cEV miR-137. Overall, cEV miR-137 had high potential as a blood-based marker of methamphetamine abstinence. cEV miR-137 retained the diagnostic power irrespective of the duration of methamphetamine abstinence or methamphetamine use. Interestingly, cEV miR-137 interacted with age: Control participants displayed an aging-dependent reduction of cEV miR-137, while methamphetamine-abstinent patients showed an aging-dependent increase in cEV miR-137. Accordingly, cEV miR-137 had variable diagnostic power depending on age, in which cEV miR-137 more effectively discriminated methamphetamine abstinence in the younger population. Duration of methamphetamine use or abstinence, cigarette smoking status, depressive disorder, or antidepressant treatment did not interact with the methamphetamine abstinence-induced reduction of cEV miR-137.

Conclusion
Our data collectively demonstrated that miR-137 in the circulating extracellular vesicles held high potential as a stable and accurate diagnostic marker of methamphetamine abstinence syndrome.
Teleocidin-producing genotype of Streptomyces clavuligerus ATCC 27064
Authors: Pivk Lukančič Petra , Drčar Tjaša , Bruccoleri Robert , Črnugelj Martin , Mrak Peter 
Published: February 2022

Abstract

Streptomyces clavuligerus is an industrially important producer of clavulanic acid (CA), a β-lactamase inhibitor which is used together with amoxicillin in one of the most widely prescribed antibacterial medicines, the co-amoxiclav. In a mid-eighties ATCC vial of S. clavuligerus ATCC 27064 culture, we have found a new genotype, which was apparently lost from the subsequent ATCC collection stocks, and has remained obscure to the scientific community. Most importantly, this genotype harbors teleocidin (lyngbyatoxin) biosynthetic genes, which are located on an enigmatic 138 kb chromosomal region and support accumulation of significant amounts of these highly toxic, tumor-promoting secondary metabolites in cultures of S. clavuligerus. While this genomic region is completely absent from all published sequences for S. clavuligerus ATCC strain, at least one of the industrial strains for commercial production of CA, originating from ATCC 27064, retained the genetic potential for production of teleocidins. The origin of teleocidin biosynthetic cluster can now be traced back to early S. clavuligerus stocks at the ATCC. Our work provides a genome sequence and a deposited monoisolate of this genotype. Given the scale of industrial use of S. clavuligerus world-wide and toxicity of teleocidins, we also discuss the environmental and safety implications and provide a method of abolishing teleocidin production without affecting productivity of CA.

Key points
• Early stocks of S. clavuligerus ATCC 27064 produce toxic teleocidins
• Teleocidin biosynthetic genes were found within a distinct S. clavuligerus genotype
• The genotype has been passed on to some industrial clavulanic acid producer strains
Quantitative classification and radiomics of [18F]FDG-PET/CT in indeterminate thyroid nodules
Authors: de Koster Elizabeth J. , Noortman Wyanne A. , Mostert Jacob M. , Booij Jan , Brouwer Catherine B. , de Keizer Bart , de Klerk John M. H. , Oyen Wim J. G. , van Velden Floris H. P. , de Geus-Oei Lioe-Fee , Vriens Dennis ,   for the EfFECTS trial study group
Published: February 2022

Abstract
Purpose
To evaluate whether quantitative [18F]FDG-PET/CT assessment, including radiomic analysis of [18F]FDG-positive thyroid nodules, improved the preoperative differentiation of indeterminate thyroid nodules of non-Hürthle cell and Hürthle cell cytology.

Methods
Prospectively included patients with a Bethesda III or IV thyroid nodule underwent [18F]FDG-PET/CT imaging. Receiver operating characteristic (ROC) curve analysis was performed for standardised uptake values (SUV) and SUV-ratios, including assessment of SUV cut-offs at which a malignant/borderline neoplasm was reliably ruled out (≥ 95% sensitivity). [18F]FDG-positive scans were included in radiomic analysis. After segmentation at 50% of SUVpeak, 107 radiomic features were extracted from [18F]FDG-PET and low-dose CT images. Elastic net regression classifiers were trained in a 20-times repeated random split. Dimensionality reduction was incorporated into the splits. Predictive performance of radiomics was presented as mean area under the ROC curve (AUC) across the test sets.

Results
Of 123 included patients, 84 (68%) index nodules were visually [18F]FDG-positive. The malignant/borderline rate was 27% (33/123). SUV-metrices showed AUCs ranging from 0.705 (95% CI, 0.601–0.810) to 0.729 (0.633–0.824), 0.708 (0.580–0.835) to 0.757 (0.650–0.864), and 0.533 (0.320–0.747) to 0.700 (0.502–0.898) in all (n = 123), non-Hürthle (n = 94), and Hürthle cell (n = 29) nodules, respectively. At SUVmax, SUVpeak, SUVmax-ratio, and SUVpeak-ratio cut-offs of 2.1 g/mL, 1.6 g/mL, 1.2, and 0.9, respectively, sensitivity of [18F]FDG-PET/CT was 95.8% (95% CI, 78.9–99.9%) in non-Hürthle cell nodules. In Hürthle cell nodules, cut-offs of 5.2 g/mL, 4.7 g/mL, 3.4, and 2.8, respectively, resulted in 100% sensitivity (95% CI, 66.4–100%). Radiomic analysis of 84 (68%) [18F]FDG-positive nodules showed a mean test set AUC of 0.445 (95% CI, 0.290–0.600) for the PET model.

Conclusion
Quantitative [18F]FDG-PET/CT assessment ruled out malignancy in indeterminate thyroid nodules. Distinctive, higher SUV cut-offs should be applied in Hürthle cell nodules to optimize rule-out ability. Radiomic analysis did not contribute to the additional differentiation of [18F]FDG-positive nodules.

Trial registration number
This trial is registered with ClinicalTrials.gov: NCT02208544 (5 August 2014), https://clinicaltrials.gov/ct2/show/NCT02208544.
Comparison of antipsychotic drug use among Dutch Youth before and after implementation of the Youth Act (2010–2019)
Authors: Bais Y. , Hermans R. A. , Schuiling-Veninga C. C. M. , Bos H. J. , Kloosterboer S. M. , de Winter B. C. M. , Simoons M. , Dieleman G. C. , Hillegers M. H. J. , Koch B. C. P. , Dierckx B. 
Published: February 2022

Abstract
Objective
The Dutch law on youth care (the Youth Act) was implemented from 2015 onwards. One of the government’s aims by implementing this new policy was de-medicalization of youths by separating youth mental healthcare from the rest of the healthcare system. A previous study conducted by our research group showed that prevalence rates of antipsychotic drug prescriptions stabilized among Dutch youth in the period 2005–2015, just before the introduction of the Youth Act. In our study, we aimed to describe antipsychotic drug use among Dutch children aged 0–19 years old before and after implementation of the Youth Act (2010–2019).

Methods
We analyzed prescription data of 7405 youths aged 0–19 years using antipsychotic drugs between 2010 and 2019, derived from a large Dutch community pharmacy-based prescription database (IADB.nl).

Results
Prevalence rates of antipsychotic drug use per thousand youths decreased significantly in youths aged 7–12 years old in 2019 compared to 2015 (7.9 vs 9.0 p < 0.05). By contrast, prevalence rates increased in adolescent females in 2019 compared to 2015 (11.8 vs 9.5 p < 0.05). Incidence rates increased significantly in adolescent youths in 2019 compared to 2015 (3.9 vs 3.0 p < 0.05), specifically among adolescent girls (4.2 per thousand in 2019 compared to 3.0 per thousand in 2015). Dosages in milligram declined for the most commonly prescribed antipsychotic drugs during the study period. The mean duration of antipsychotic drug use in the study period was 5.7 (95% CI 5.2–6.2) months.

Conclusion
Despite the aim of the Youth Act to achieve de-medicalization of youths, no clear reduction was observed in prevalence rates of antipsychotic drugs or treatment duration in all subgroups. Prevalence rates even increased in adolescent females.
Separate collection and bio-waste valorization in the Italian poultry sector by material flow analysis
Authors: Bux Christian , Amicarelli Vera 
Published: February 2022

AbstractPoultry meat production and consumption face several challenges under economic, social and environmental perspectives, and increasing concerns are associated with food loss and waste minimization. One of the main issues is related to the absence of a homogeneous and standardized separate collection of bio-waste at country level, which makes chicken bones, skin and food waste valorization a challenging goal. The present research, implementing the material flow analysis to the Italian poultry sector, aims at measuring poultry-related co-products and by-products, exploring food waste, chicken bones and skin and the current trends in the Italian bio-waste separate collection. Then, it discusses alternative separate collection strategies and sustainable consumption habits. Data have been collected according to a research triangulation approach, whereas system boundaries consider slaughterhouse, distribution and final consumption stage. It emerges that more than 1.50 Mt of live animals have been processed to obtain 0.46 Mt of fresh meat and 0.76 Mt of co-products and by-products, of which more than 0.32 Mt are represented by chicken bones and skin. In addition, more than 0.15 Mt of food waste have been recorded. The research adds an extra step towards the identification of awareness campaigns and separate collection strategies at national level.
Finite Element Systems for Vector Bundles: Elasticity and Curvature
Authors: Christiansen Snorre H. , Hu Kaibo 
Published: February 2022

AbstractWe develop a theory of finite element systems, for the purpose of discretizing sections of vector bundles, in particular those arising in the theory of elasticity. In the presence of curvature, we prove a discrete Bianchi identity. In the flat case, we prove a de Rham theorem on cohomology groups. We check that some known mixed finite elements for the stress–displacement formulation of elasticity fit our framework. We also define, in dimension two, the first conforming finite element spaces of metrics with good linearized curvature, corresponding to strain tensors with Saint-Venant compatibility conditions. Cochains with coefficients in rigid motions are given a key role in relating continuous and discrete elasticity complexes.
The additional use of strain measurements for timing of treatment in severe aortic regurgitation
Authors: Sinning Christoph 
Published: February 2022

AbstractAssessment of severity and need for intervention in clinical practice often is in need for a thorough echocardiography regarding function of the ventricles and additional valvular dysfunction. Despite the indications recommended in the current guidelines, there is still need for further research to identify patients with a severe valvular dysfunction but a potential reversible status regarding the function of the ventricles. Strain imaging is suggested in the current literature to be an additional tool to identify ventricular dysfunction in the setting of preserved left ventricular ejection fraction.
Toxicity screening of bisphenol A replacement compounds: cytotoxicity and mRNA expression in LMH 3D spheroids
Authors: Sharin Tasnia , Crump Doug , O’Brien Jason M. 
Published: February 2022

AbstractPreviously, we showed that the chicken LMH cell line cultured as 3D spheroids may be a suitable animal free alternative to primary chicken embryonic hepatocytes (CEH) for avian in vitro chemical screening. In this study, cytotoxicity and mRNA expression were determined in LMH 3D spheroids following exposure to bisphenol A (BPA), five BPA replacement compounds (BPF, TGSH, DD-70, BPAF, BPSIP), and 17β estradiol (E2). Results were compared to an earlier study that evaluated the same endpoints for these chemicals in CEH. BPA and the replacement compounds had LC50 values ranging from 16.6 to 81.8 μM; DD-70 and BPAF were the most cytotoxic replacements (LC50 = 17.23 ± 4.51 and 16.6 ± 4.78 μM). TGSH and DD-70 modulated the greatest number of genes, although fewer than observed in CEH. Based on the expression of apovitellenin and vitellogenin, BPAF was the most estrogenic compound followed by BPF, BPSIP, and BPA. More estrogen-responsive genes were modulated in LMH spheroids compared to CEH. Concentration-dependent gene expression revealed that DD-70 and BPAF altered genes related to lipid and bile acid regulation. Overall, cytotoxicity and clustering of replacements based on gene expression profiles were similar between LMH spheroids and CEH. In addition to generating novel gene expression data for five BPA replacement compounds in an in vitro avian model, this research demonstrates that LMH spheroids may represent a useful animal free alternative for avian toxicity testing.
Effect of transport infrastructure development on selected components of the environment of inner-city river valley and the possibility of its revitalization (Lublin, Poland)
Authors: Zubala Tomasz 
Published: February 2022

AbstractThe study covered an urban river valley, strongly transformed due to the transport infrastructure development. The paper evaluates changes in spatial management of the valley section passing through the city centre that occurred during the past two centuries (long-term and short-term phenomena) as well as their effect on selected environmental components. The basic spatial analyses were carried out with the use of specialized software, cartographic materials and photographic and descriptive documentation of the studied area (archival data). The most unfavourable changes in the potential of the valley environment occurred over the past few decades. They are manifested in a considerable deterioration of landscape values as well as the quantity and quality of water resources. Relationship was identified between water quality and intensity of motor vehicle traffic near the river. Despite the progressing urbanisation of the valley, revitalisation procedures can be still carried out and attractive space in the city centre can be created. In order to demonstrate the reasonableness of the presented concept, the paper suggests that urban succession should be incorporated as a term covering time trends and accumulated transformations. It can be helpful in analysing and determining the directions of development in disputable situations.
Using Autonomous Teams to Improve Quality of Life of People with Intellectual Disabilities
Authors: Blížkovská Marie , Martínez-Tur Vicente , Pătras Luminita , Moliner Carolina , Gracia Esther , Ramos José 
Published: February 2022

AbstractThis research study tested an intervention, based on autonomous teams and the service co-creation approach, to improve the social inclusion and self-determination (as indicators of quality of life) of people with intellectual disability. Members of the autonomous teams (professionals, people with intellectual disability, and family members) cooperated to plan and carry out a project. A total of 72 autonomous teams participated (experimental condition). Each team designed its action plan to be implemented during eight weeks. Three measurement times were used: before the intervention (T1), four (T2) and eight (T3) weeks after the starting point. Family members (experimental group, N = 117; control group, N = 187) reported on the social inclusion and self-determination of their relative with intellectual disability. Scores in the control group remained stable over time, whereas scores in the experimental group changed significantly. Social inclusion increased (T2), and later stabilized (T3). By contrast, self-determination increased (T2), but this improvement was subsequently reduced (T3).
Treatment of Persistent Large Gastrocutaneous Fistulas After Bariatric Surgery: Preliminary Experience with Endoscopic Kehr’s T-Tube Placement
Authors: Liagre Arnaud , Queralto Michel , Levy Jonathan , Combis Jean Marc , Peireira Paulo , Buchwald Jane N. , Juglard Gildas , Petrucciani Niccolò , Martini Francesco 
Published: February 2022

Abstract

Purpose
Post-bariatric surgery gastrocutaneous fistula is a chronic leak with an incidence of 1.7 to 4.0% and no standardized management. A large gastrocutaneous fistula (LGCF) is not indicated for treatment with pigtail drains. We aimed to evaluate results of a novel treatment using endoscopic Kehr’s T-tube placement.

Methods
Only patients with a postoperative LGCF duration of > 10 days and a flow rate of > 50 cc by external drainage after revisional surgery for sepsis were included. Endoscopic placement of Kehr’s T-tube was performed. Patients had been reoperated with wash and drainage for severe sepsis after initial bariatric surgery in which no fistula had been discovered. Patients not reoperated, or with a fistula requiring intraoperative Kehr’s T-tube placement, or a pigtail drain were excluded. Primary outcomes were endoscopic characteristics and results (LGCF closure rate, Kehr T-tube retention time, etc.).

Results
The study group included 12 women, 2 men; body mass index 43.1 ± 4.5 kg/m2. Interventions were SG (7), RYGB (2), OAGB (4), and SADI-S (1). Endoscopic assessment was carried out after a mean of 33.2 ± 44.3 days after the bariatric procedure. The mean fistula orifice diameter was 2.0 ± 0.9 cm. Kehr’s T-tube was positioned at a mean 51.5 ± 54.8 days after the bariatric procedure. T-tube tolerance was excellent. Mean additional days: hospitalization, 34.4 ± 27.0; T-tube retention, 86.4 ± 73.1; fistula healing, 139.9 ± 111.5, LGCF closure rate, 92.9%. Complications: 1 pulmonary embolism, 2 T-tube migrations,1 drain-path bleed, 1 skin abscess. No mortality.

Conclusions
Endoscopic Kehr’s T-tube placement was successful in closing persistent post-bariatric surgery LGCF in 92.9% of patients.

Graphical abstract