bluuebunny/crossref_metadata_2025_split huggingface dataset:
A real-world study on SGLT2 inhibitors and diabetic kidney disease progression
Authors: Liu Allen Yan Lun , Low Serena , Yeoh Ester , Lim Eng Kuang , Renaud Claude Jeffrey , Teoh Selene Tse Yen , Tan Grace Feng Ling , Chai Chung Cheen , Liu Bo , Subramaniam Tavintharan , Sum Chee Fang , Lim Su Chi 
Published: February 2022

ABSTRACTBackgroundRandomized controlled trials have demonstrated the benefits of sodium–glucose cotransporter 2 inhibitors (SGLT2is) in people with type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). However, real-world data on CKD progression and the development of end-stage kidney disease (ESKD) remains scarce. Our aim was to study renal outcomes of people with diabetic kidney disease (DKD) using SGLT2is in a highly prevalent DKD population.MethodsBetween 2016 and 2019 we recruited T2DM patients in the renal and diabetic clinics in a regional hospital in Singapore. Patients prescribed SGLT2is were compared with those on standard anti-diabetic and renoprotective treatment. The outcome measures were CKD progression [a ≥25% decrease from baseline and worsening of estimated glomerular filtration rate (eGFR) categories according to the Kidney Disease: Improving Global Outcomes guidelines] and ESKD (eGFR &lt;15 mL/min/1.73 m2).ResultsWe analysed a total of 4446 subjects; 1598 were on SGLT2is. There was a significant reduction in CKD progression {hazard ratio [HR] 0.60 [95% confidence interval (CI) 0.49–0.74]} with SGLT2is. The HR for eGFR ≥45 mL/min/1.73 m2 and 15–44 mL/min/1.73 m2 was 0.60 (95% CI 0.47–0.76) and 0.43 (95% CI 0.23–0.66), respectively. There was also a reduction in risk for developing ESKD for the entire cohort [HR 0.33 (95% CI 0.17–0.65)] and eGFR 15–44 mL/min/1.73 m2 [HR 0.24 (95% CI 0.09–0.66)]. Compared with canagliflozin and dapagliflozin, empagliflozin showed a sustained risk reduction of renal outcomes across CKD stages 1–4.ConclusionsThis real-world study demonstrates the benefits of SGLT2is on CKD progression and ESKD. The effect is more pronounced in moderate to advanced CKD patients.
When dystrophia meets ischaemia: a case report on cardiac involvement of myotonic dystrophy type 2 and successful arrhythmia elimination after catheter ablation
Authors: Guckel Denise , Farr Martin , Sommer Philipp , Sohns Christian 
Published: February 2022

Abstract

Background
This case reviews the cardiac involvement of myotonic dystrophy type 2 in terms of ventricular arrhythmias (VAs) and individual myocardial scar formation as target for catheter ablation.

Case summary
A 62-year-old woman with myotonic dystrophy type 2 and a severely reduced left ventricular ejection fraction (25%) presented with recurrent episodes of VAs and consecutive implantable cardioverter-defibrillator therapies. The patient already underwent two VA ablation attempts focusing on an ischaemia-related arrhythmia substrate in the left ventricle. The patient was scheduled for repeat ablation after the progression of coronary artery disease was ruled out. Interestingly bipolar voltage as well as activation mapping revealed an arrhythmia substrate along with the basal and inferior aspects of the right ventricle (RV). Catheter ablation of this scarred area in the RV resulted in specific termination of the VAs. Due to end-stage heart failure, key heart transplant criteria were met. The patient was evaluated for heart transplantation and added to the waiting list. Hitherto, no further VAs were documented during follow-up.

Discussion
As these patients present with specific dystrophia-related arrhythmia substrates, we propose pre-procedural visualization of dystrophy-associated arrhythmia substrates using cardiac magnetic resonance imaging allowing for personalized ablation approaches in these patients.
A case report of left ventricular thrombus formation following aggressive decongestion treatment
Authors: Mystakidi Vasiliki Chara , Oikonomou Evangelos , Katsianos Efstratios , Vavuranakis Manolis 
Published: February 2022

Abstract

Background
Intracardiac thrombi are a complication associated with cardiomyopathies. In heart failure with reduced ejection fraction, there is a hypercoagulable state that can increase the incidence of left ventricular thrombus and result in higher risk of thromboembolism, either manifested as stroke or as peripheral thromboembolic event. Haemoconcentration following decongestion treatment may enhance blood viscosity.

Case summary
A 63-year-old man with known long-standing heart failure (HF) of ischaemic aetiology and not any prothrombotic risk, admitted with congestive HF and treated with aggressive decongestion treatment with intravenous loop diuretics. During his hospital stay, and following decongestion and haemoconcentration, a left ventricular (LV) intracardiac thrombus formation was detected by echocardiography, which occurred in the absence of a recent myocardial infarction or adverse LV remodelling. The patient was treated with oral anticoagulation therapy. There was a complete resolution of the thrombus on repeat transthoracic echocardiography after 4 weeks of treatment.

Discussion
Aggressive decongestive treatment, haemoconcentration and increased blood viscosity following HF decompensation may serve as a trigger for intracardiac thrombus formation under the appropriate prothrombotic background. Appropriate primary antithrombotic prophylaxis is an issue raised concerns and vulnerable patients need closed clinical and imaging follow-up.
Human leukocyte antigen mismatch on lung transplantation outcomes
Authors: Firoz Ahad , Kashem Mohammed , Zhao Huaqing , Geier Steven , Toyoda Yoshiya 
Published: February 2022

Abstract

OBJECTIVES
Human leucocyte antigen (HLA) mismatch is a known risk factor for renal transplantation; however, there are conflicting and limited data on its ramifications within lung transplantation (LTx). Therefore, our study evaluated the effects of total HLA, HLA-A, -B and -DR mismatches on LTx outcomes.

METHODS
We retrospectively examined the United Network for Organ Sharing database for adult patients who had undergone LTx for the first time between January 2005 and July 2021. Total HLA mismatch (0–3, 4, 5 and 6) and HLA locus mismatch (0–1 and 2) were analysed, with the end points of interest being mortality and bronchiolitis obliterans syndrome (BOS) development.

RESULTS
Kaplan–Meier curve analysis found a significant difference in both overall survival (n = 27 651; 11 830 events) and BOS development (n = 25 444; 8901 events) for the total number of HLA (P &lt; 0.001, P &lt; 0.001), HLA-A (P &lt; 0.001, P = 0.006) and HLA-DR (P &lt; 0.001, P &lt; 0.001) mismatches. With reference to 0–3 total HLA mismatches, multivariable Cox regression model found that 6 mismatches had an increased risk of mortality (P = 0.002) while 4 (P = 0.010), 5 (P = 0.007) and 6 (P &lt; 0.001) mismatches had an increased risk of BOS. HLA-B mismatch was not associated with an increased mortality (P = 0.975) or BOS risk (P = 0.512).

CONCLUSIONS
This study demonstrates a significant relationship between increased HLA mismatches and BOS development, with decreased overall survival only apparent with 6 mismatches. HLA-A and -DR mismatches were associated with an increased risk of mortality and BOS development compared to groups with at least 1 locus match.
Cooperative microbial interactions mediate community biogeochemical responses to saltwater intrusion in wetland soils
Authors: Berrier David J , Neubauer Scott C , Franklin Rima B 
Published: February 2022

AbstractIn freshwater wetlands, competitive and cooperative interactions between respiratory, fermentative and methanogenic microbes mediate the decomposition of organic matter. These interactions may be disrupted by saltwater intrusion disturbances that enhance the activity of sulfate-reducing bacteria (SRB), intensifying their competition with syntrophic bacteria and methanogens for electron donors. We simulated saltwater intrusion into wetland soil microcosms and examined biogeochemical and microbial responses, employing metabolic inhibitors to isolate the activity of various microbial functional groups. Sulfate additions increased total carbon dioxide production but decreased methane production. Butyrate degradation assays showed continued (but lower) levels of syntrophic metabolism despite strong demand by SRB for this key intermediate decomposition product and a shift in the methanogen community toward acetoclastic members. One month after removing SRB competition, total methane production recovered by only ∼50%. Similarly, butyrate assays showed an altered accumulation of products (including less methane), although overall rates of syntrophic butyrate breakdown largely recovered. These effects illustrate that changes in carbon mineralization following saltwater intrusion are driven by more than the oft-cited competition between SRB and methanogens for shared electron donors. Thus, the impacts of disturbances on wetland biogeochemistry are likely to persist until cooperative and competitive microbial metabolic interactions can recover fully.
Sigma regulatory network in Rhodococcus erythropolis CCM2595
Authors: Štěpánek Václav , Dostálová Hana , Busche Tobias , Blumenstein Jan , Grulich Michal , Plašil Lukáš , Rucká Lenka , Nešvera Jan , Pátek Miroslav 
Published: February 2022

Abstract
The aim of this investigation was to discover the promoters that drive expression of the sig genes encoding sigma factors of RNA polymerase in Rhodococcus erythropolis CCM2595 and classify these promoters according to the sigma factors which control their activity. To analyze the regulation of major sigma factors, which control large regulons that also contain genes expressed under exponential growth and non-stressed conditions, we used the R. erythropolis CCM2595 culture, which grew rapidly in minimal medium. The transcriptional start sites (TSSs) of the genes sigA, sigB, sigD, sigE, sigG, sigH, sigJ, and sigK were detected by primary 5′-end-specific RNA sequencing. The promoters localized upstream of the detected TSSs were defined by their −35 and −10 elements, which were identical or closely similar to these sequences in the related species Corynebacterium glutamicum and Mycobacterium tuberculosis. Regulation of the promoter activities by different sigma factors was demonstrated by two independent techniques (in vivo and in vitro). All analyzed sig genes encoding the sigma factors with extracytoplasmic function (ECF) were found to be also driven from additional housekeeping promoters. Based on the classification of the sig gene promoters, a model of the basic sigma transcriptional regulatory network in R. erythropolis was designed.
Agent-Based Covid economics (ABC): Assessing non-pharmaceutical interventions and macro-stabilization policies
Authors: Delli Gatti Domenico , Reissl Severin 
Published: February 2022

Abstract
We employ a new macroeconomic–epidemiological agent-based model to evaluate the “lives vs livelihoods” trade-off brought to the fore by coronavirus disease (Covid-19). An infectious disease spreads across the network of agents’ social and economic contacts and feeds back on the economic dimension of the model through various channels such as employment and consumption demand. Under a lockdown (LD) scenario, the model is able to closely reproduce the epidemiological dynamics of the first wave of the Covid-19 epidemic in the Lombardy region of Italy. We find that, in the presence of non-pharmaceutical interventions, there is no trade-off between lives and macroeconomic losses as a stricter LD eventually leads to superior outcomes along both dimensions. We also evaluate the efficacy of various macroeconomic stabilization policies designed to counteract the downturn generated by the epidemic and LD measures. In an agent-based setting we gain additional insights on the way in which such policies impact not only on gross domestic product but also, for instance, on firms’ defaults and relative prices. Liquidity support for firms, a short-time working scheme with compensation for workers, and direct transfer to households stand out as the most effective policy tools.
Combining Cellular Immunology With RNAseq to Identify Novel Chlamydia T-Cell Subset Signatures
Authors: Johnson Raymond M , Asashima Hiromitsu , Mohanty Subhasis , Shaw Albert C 
Published: February 2022

AbstractChlamydia trachomatis serovars A–L cause important diseases of the eyes and reproductive tract by infecting epithelium lining those organs. A major hurdle for vaccine trials is finding a surrogate biomarker for protective immunity. Investigational data argues for T-cell biomarker(s) reflecting mucosal adaption, cytokine polarization, B-cell help, antibacterial effector mechanisms, or some combination thereof. A human investigation and 2 mouse studies link IL-13 to protection from infection/immunopathology. We performed RNAseq on T cells resident in spleens and genital tracts of naturally immune mice. CD4 signatures were consistent with helper function that differed by site including a genital tract-specific Fgl2 signal. The genital tract CD8 signature featured IL-10 and promotion of healing/scarring with a unique transcription of granzyme A. The RNAseq data was used to refine previously published CD4γ13 and CD8γ13 transcriptomes derived from protective T-cell clones, potentially identifying practicable T-cell subset signatures for assessing Chlamydia vaccine candidates.
Influence of context availability and soundness in predicting soil moisture using the Context-Aware Data Mining approach
Authors: Avram Anca , Matei Oliviu , Pintea Camelia-M , Pop Petrica C 
Published: February 2022

AbstractKnowing the level of quality from which the context is no longer valuable in a Context-Aware Data Mining (CADM) system is an important information. The main goal of this research is to study the variations of the predictions in case of different levels of noise and missing context data in practical scenarios for predicting soil moisture. The research has been performed on two locations from the Transylvanian Plain, Romania and two locations from Canada. The values predicted for the soil moisture were compared in mixed scenarios that vary the quantity of noise and missing context data. The studied behavior was performed using Deep Learning, Decision Tree and Gradient Boosted Tree machine learning algorithms. It has been shown that when using the air temperature as context for predicting soil moisture, variations of noise and missing data do not influence the results proportionally with the levels of noise and missing data applied. Also, Gradient Boosted Tree algorithm proves to be the best algorithm from the ones studied, to be considered when predicting soil moisture with the CADM approach.
A summary of the statistical aspects of the procedures for resolving potential employment discrimination recently issued by the Office of Federal Contract Compliance along with a commentary
Authors: Gastwirth Joseph L 
Published: February 2022

AbstractOn 5 November 2020, the Office of Federal Contract Compliance in the Department of Labor issued new rules codifying the procedures it will use to resolve equal employment issues. First, this article summarizes the new rules focusing on how the agency will use and evaluate statistical evidence in its monitoring of government contractors’ compliance with equal employment laws. After noting the diminished role of statistical evidence in the new rules, the likely effect of them on the use of statistical data and analyses in equal employment proceedings are explored. The logical and statistical reasoning underlying several aspects of the new rules is also questioned. Because the new rules refer to a report of the Chamber of Commerce that, in part, criticized the agency from bringing a case against a firm, data from the case are re-analyzed. The statistical analysis provides support for the position of OFCCP.
A multi-wavelength view of distinct accretion regimes in the pulsating ultraluminous X-ray source NGC 1313 X-2
Authors: Sathyaprakash R , Roberts T P , Grisé F , Kaaret P , Ambrosi E , Done C , Gladstone J C , Kajava J J E , Soria R , Zampieri L 
Published: February 2022

ABSTRACTNGC 1313 X-2 is one of the few known pulsating ultraluminous X-ray sources (PULXs), and so is thought to contain a neutron star that accretes at highly super-Eddington rates. However, the physics of this accretion remains to be determined. Here, we report the results of two simultaneous XMM–Newton and HST observations of this PULX taken to observe two distinct X-ray behaviours as defined from its Swift light curve. We find that the X-ray spectrum of the PULX is best described by the hard ultraluminous regime during the observation taken in the lower flux, lower variability amplitude behaviour; its spectrum changes to a broadened disc during the higher flux, higher variability amplitude epoch. However, we see no accompanying changes in the optical/UV fluxes, with the only difference being a reduction in flux in the near-infrared (NIR) as the X-ray flux increased. We attempt to fit irradiation models to explain the UV/optical/IR fluxes but they fail to provide meaningful constraints. Instead, a physical model for the system leads us to conclude that the optical light is dominated by a companion O/B star, albeit with an IR excess that may be indicative of a jet. We discuss how these results may be consistent with the precession of the inner regions of the accretion disc leading to changes in the observed X-ray properties, but not the optical, and whether we should expect to observe reprocessed emission from ULXs.
Uncertainties associated with the backward integration of dwarf satellites using simple parametric potentials
Authors: D’Souza Richard , Bell Eric F 
Published: February 2022

ABSTRACT
In order to backward integrate the orbits of Milky Way (MW) dwarf galaxies, much effort has been invested in recent years to constrain their initial phase-space coordinates. Yet equally important are the assumptions on the potential that the dwarf galaxies experience over time, especially given the fact that the MW is currently accreting the Large Magellanic Cloud (LMC). In this work, using a dark-matter-only zoom-in simulation, we test whether the use of common parametric forms of the potential is adequate to successfully backward integrate the orbits of the subhaloes from their present-day positions. We parametrize the recovered orbits and compare them with those from the simulations. We find that simple symmetric parametric forms of the potential fail to capture the complexities and the inhomogeneities of the true potential experienced by the subhaloes. More specifically, modelling a recent massive accretion like that of the LMC as a sum of two spherical parametric potentials leads to substantial errors in the recovered parameters of the orbits. These errors rival those caused due to (a) a 30 per cent uncertainty in the virial mass of the MW and (b) not modelling the potential of the recently accreted massive satellite. Our work suggests that (i) the uncertainties in the parameters of the recovered orbits of some MW dwarfs may be underestimated and that (ii) researchers should characterize the uncertainties inherent to their choice of integration techniques and assumptions of the potential against cosmological zoom-in simulations of the MW, which include a recently accreted LMC.
Estimating linear radiance indicators from the zenith night-sky brightness: on the Posch ratio for natural and light-polluted skies
Authors: Bará Salvador , Pérez-Couto Xabier , Falchi Fabio , Kocifaj Miroslav , Masana Eduard 
Published: February 2022

ABSTRACT
Estimating the horizontal irradiance from measurements of the zenith night-sky radiance is a useful operation for basic and applied studies in observatory site assessment, atmospheric optics, and environmental sciences. The ratio between these two quantities, also known as the Posch ratio, has previously been studied for some canonical cases and reported for a few observational sites. In this work we (a) generalize the Posch ratio concept, extending it to any pair of radiance-related linear indicators, (b) describe its main algebraic properties, and (c) provide analytical expressions and numerical evaluations for its three basic night-time components (moonlight, starlight and other astrophysical light sources, and artificial light). We show that the horizontal irradiance (or any other linear radiance indicator) is generally correlated with the zenith radiance, enabling its estimation from zenith measurements if some a priori information on the atmospheric state is available.
The RecD2 helicase balances RecA activities
Authors: Ramos Cristina , Hernández-Tamayo Rogelio , López-Sanz María , Carrasco Begoña , Serrano Ester , Alonso Juan C , Graumann Peter L , Ayora Silvia 
Published: February 2022

AbstractDNA helicases of the RecD2 family are ubiquitous. Bacillus subtilis RecD2 in association with the single-stranded binding protein SsbA may contribute to replication fork progression, but its detailed action remains unknown. In this work, we explore the role of RecD2 during DNA replication and its interaction with the RecA recombinase. RecD2 inhibits replication restart, but this effect is not observed in the absence of SsbA. RecD2 slightly affects replication elongation. RecA inhibits leading and lagging strand synthesis, and RecD2, which physically interacts with RecA, counteracts this negative effect. In vivo results show that recD2 inactivation promotes RecA–ssDNA accumulation at low mitomycin C levels, and that RecA threads persist for a longer time after induction of DNA damage. In vitro, RecD2 modulates RecA-mediated DNA strand-exchange and catalyzes branch migration. These findings contribute to our understanding of how RecD2 may contribute to overcome a replicative stress, removing RecA from the ssDNA and, thus, it may act as a negative modulator of RecA filament growth.
Flavodiiron proteins enhance the rate of CO2 assimilation in Arabidopsis under fluctuating light intensity
Authors: Basso Leonardo , Sakoda Kazuma , Kobayashi Ryouhei , Yamori Wataru , Shikanai Toshiharu 
Published: February 2022

Abstract
The proton concentration gradient (ΔpH) and membrane potential (Δψ) formed across the thylakoid membrane contribute to ATP synthesis in chloroplasts. Additionally, ΔpH downregulates photosynthetic electron transport via the acidification of the thylakoid lumen. K+ exchange antiporter 3 (KEA3) relaxes this downregulation by substituting ΔpH with Δψ in response to fluctuation of light intensity. In the Arabidopsis (Arabidopsis thaliana) line overexpressing KEA3 (KEA3ox), the rate of electron transport is elevated by accelerating the relaxation of ΔpH after a shift from high light (HL) to low light. However, the plant cannot control electron transport toward photosystem I (PSI), resulting in PSI photodamage. In this study, we crossed the KEA3ox line with the line (Flavodiiron [Flv]) expressing the Flv proteins of Physcomitrium patens. In the double transgenic line (Flv-KEA3ox), electrons overloading toward PSI were pumped out by Flv proteins. Consequently, photodamage of PSI was alleviated to the wild-type level. The rate of CO2 fixation was enhanced in Flv and Flv-KEA3ox lines during HL periods of fluctuating light, although CO2 fixation was unaffected in any transgenic lines in constant HL. Upregulation of CO2 fixation was accompanied by elevated stomatal conductance in fluctuating light. Consistent with the results of gas exchange experiments, the growth of Flv and Flv-KEA3ox plants was better than that of WT and KEA3ox plants under fluctuating light.