bluuebunny/crossref_metadata_2025_split huggingface dataset:
N6‐methyladenosine reader YTHDF3 regulates melanoma metastasis via its ‘executor' LOXL3
Authors: Shi Hao‐ze , Xiong Jing‐shu , Gan Lu , Zhang Ying , Zhang Cong‐cong , Kong Ying‐qi , Miao Qiu‐ju , Tian Cui‐cui , Li Rong , Liu Jin‐quan , Zhang Er‐jia , Bu Wen‐bo , Wang Yan , Cheng Xian‐feng , Sun Jian‐fang , Chen Hao 
Published: November 2022

BackgroundA number of studies have demonstrated that N6‐methyladenosine (m6A) plays a vital role in the pathological process of various tumours. Recently, it was found that m6A writers or erasers affect the tumourigenesis of melanoma. However, the relationship between m6A readers such as YTH domain family (YTHDF) proteins and melanoma was still elusive.MethodsRT‐qPCR, Western blot and immunohistochemistry were conducted to measure the expression level of YTH N6–methyladenosine RNA binding protein 3 (YTHDF3) and lysyl oxidase–like 3 (LOXL3) in melanoma tissues and cells. The effects of YTHDF3 and LOXL3 on melanoma were verified in vitro and in vivo. Multi‐omics analysis including RNA‐seq, MeRIP‐seq, RIP‐seq and mass spectrometry analyses was performed to identify the target. The interaction between YTHDF3 and LOXL3 was verified by RT‐PCR, Western blot, MeRIP‐qPCR, RIP‐qPCR and CRISPR‐Cas13b‐based epitranscriptome engineering.ResultsIn this study, we found that m6A reader YTHDF3 could affect the metastasis of melanoma both in vitro and in vivo. The downstream targets of YTHDF3, such as LOXL3, phosphodiesterase 3A (PDE3A) and chromodomain helicase DNA‐binding protein 7 (CHD7) were identified by means of RNA‐seq, MeRIP‐seq, RIP‐seq and mass spectrometry analyses. Besides, RT‐qPCR, Western blot, RIP‐qPCR and MeRIP‐qPCR were performed for subsequent validation. Among various targets of YTHDF3, LOXL3 was found to be the optimal target of YTHDF3. With the application of CRISPR–Cas13b‐based epitranscriptome engineering, we further confirmed that the transcript of LOXL3 was captured and regulated by YTHDF3 via m6A binding sites. YTHDF3 augmented the protein expression of LOXL3 without affecting its mRNA level via the enrichment of eukaryotic translation initiation factor 3 subunit A (eIF3A) on the transcript of LOXL3. LOXL3 downregulation inhibited the metastatic ability of melanoma cells, and overexpression of LOXL3 ameliorated the inhibition of melanoma metastasis caused by YTHDF3 downregulation.ConclusionsThe YTHDF3‐LOXL3 axis could serve as a promising target to be interfered with to inhibit the metastasis of melanoma.
Data‐centric approaches in the Internet of Vehicles: A systematic review on techniques, open issues, and future directions
Authors: Partovi Zahra , Zarei Mani , Rahmani Amir Masoud 
Published: November 2022

SummaryThe Internet of Vehicles (IoV) is an emerging network of connected vehicles as a branch of dynamic objects in the Internet of Things (IoT) ecosystem. With the rapid development of IoV, real‐time data‐centric applications would be a significant concern in academia and industry to promote efficiency and realize modern services in such high dynamic networks. In this paper, we aim to present a systematic literature review (SLR) for the IoV networks to investigate the different attitudes in the field of data‐centric approaches. This paper systematically categorizes the 48 recent articles on data‐driven techniques in the IoV field published from 2017 to March 2022. A complete technical taxonomy is presented for the data‐centric approaches in IoV according to the content of current studies. Collected methods are chosen with the SLR process, and they are investigated considering some technical classifications including IoV security, data traffic, vehicular social network, data propagation, energy, and multimedia categories. The achievements, drawbacks, and new findings of studies are carefully investigated for addressing the deficiencies, as well as emphasizing future research direction and open issues of data‐driven approaches in IoV.
Performance of the heart failure risk scores in predicting 1 year mortality and short‐term readmission of patients
Authors: Bo Xiangwei , Zhang Yahao , Liu Yang , Kharbuja Naresh , Chen Lijuan 
Published: November 2022

AbstractAimsThe aim of this study was to assess the performance of these main scores in predicting prognosis in patients with heart failure (HF).Methods and resultsA total of 2008 patients who were admitted to the Fourth People's Hospital of Zigong, Sichuan, from December 2016 to June 2019 and diagnosed with HF were included in the study. We compared the prognostic predictive performance of Seattle Heart Failure Model (SHFM), Meta‐Analysis Global Group in Chronic Heart Failure (MAGGIC‐HF) risk score, Get With the Guidelines‐Heart Failure programme (GWTG‐HF), Acute Study of Clinical Effectiveness of Nesiritide in Decompensated Heart Failure (ASCEND) risk scores, the Acute Decompensated Heart Failure National Registry (ADHERE) model, Barcelona Bio‐Heart Failure (BCN‐Bio‐HF) risk calculator, and Gruppo Italiano per lo Studio della Streptochinasi nell'Infarto Miocardico‐Heart Failure (GISSI‐HF) for the endpoints. The primary endpoint was 1 year all‐cause mortality and the secondary endpoint was the incidence of 28 day readmission post‐discharge. At 1 year follow‐up, 44 (2.21%) patients with HF died. Discrimination analyses showed that all risk scores performed reasonably well in predicting 1 year mortality, with areas under the receiver operating characteristic curve (AUCs) fluctuating between 0.757 and 0.822. GISSI‐HF showed the best discrimination with the AUC of 0.822 (0.768–0.876), followed by MAGGIC‐HF, BCN‐Bio‐HF, ASCEND, SHFM, GWTG‐HF, and ADHERE with AUCs of 0.819 (0.756–0.883), 0.812 (0.758–0.865), 0.802 (0.742–0.862), 0.787 (0.725–0.849), 0.762 (0.684–0.840), and 0.757 (0.681–0.833), respectively. All risk scores were similarly predictive of 28 day emergency readmissions, with AUCs fluctuating between 0.609 and 0.680. Overestimation of mortality occurred in all scores except the ASCEND. The risk scores remained with good prognostic discrimination in patients with biventricular HF and in the subgroup of patients taking angiotensin‐converting enzyme inhibitor/angiotensin II receptor blocker.ConclusionsCurrently assessed risk scores have limited clinical utility, with fair accuracy and calibration in assessing patients' 1 year risk of death and poor accuracy in assessing patients' risk of readmission. There is a need to incorporate more patient‐level information, use more advanced technologies, and develop models for different subgroups of patients to achieve more practical, innovative, and accurate risk assessment tools.
Identification of essential tremor based on resting‐state functional connectivity
Authors: Zhang Xueyan , Chen Huiyue , Zhang Xiaoyu , Wang Hansheng , Tao Li , He Wanlin , Li Qin , Cheng Oumei , Luo Jing , Man Yun , Xiao Zheng , Fang Weidong 
Published: November 2022

AbstractCurrently, machine‐learning algorithms have been considered the most promising approach to reach a clinical diagnosis at the individual level. This study aimed to investigate whether the whole‐brain resting‐state functional connectivity (RSFC) metrics combined with machine‐learning algorithms could be used to identify essential tremor (ET) patients from healthy controls (HCs) and further revealed ET‐related brain network pathogenesis to establish the potential diagnostic biomarkers. The RSFC metrics obtained from 127 ET patients and 120 HCs were used as input features, then the Mann–Whitney U test and the least absolute shrinkage and selection operator (LASSO) methods were applied to reduce feature dimensionality. Four machine‐learning algorithms were adopted to identify ET from HCs. The accuracy, sensitivity, specificity and the area under the curve (AUC) were used to evaluate the classification performances. The support vector machine, gradient boosting decision tree, random forest and Gaussian naïve Bayes algorithms could achieve good classification performances with accuracy at 82.8%, 79.4%, 78.9% and 72.4%, respectively. The most discriminative features were primarily located in the cerebello‐thalamo‐motor and non‐motor circuits. Correlation analysis showed that two RSFC features were positively correlated with tremor frequency and four RSFC features were negatively correlated with tremor severity. The present study demonstrated that combining the RSFC matrices with multiple machine‐learning algorithms could not only achieve high classification accuracy for discriminating ET patients from HCs but also help us to reveal the potential brain network pathogenesis in ET.
The S&P 500 index inclusion effect: Evidence from the options market
Authors: Coakley Jerry , Dotsis George , Kourtis Apostolos , Psychoyios Dimitris 
Published: November 2022

AbstractThis paper employs forward‐looking information from the options market to shed light on the comovement implications of S&P 500 index inclusion events over the 1997–2020 period. To this end, we test if forward‐looking implied betas impound significant pre‐inclusion information that is not embedded in historical betas. The empirical results show that the increase in post‐inclusion implied betas is significantly smaller than the corresponding increase in historical or hybrid betas. In most cases, changes in implied betas show no evidence of excess comovement after S&P 500 index inclusion. Our findings suggest that added stocks experience changes prior to the index addition announcement and this leads to the ‘index inclusion effect’ reported to the literature.
Effect of prosocial behavior on school bullying victimization among children and adolescents: Peer and student–teacher relationships as mediators
Authors: Fu Xinyuan , Li Shuxian , Shen Chen , Zhu Keke , Zhang Mei , Liu Yaqian , Zhang Mengyuan 
Published: November 2022

AbstractIntroductionIn this study, we examined the relationship between prosocial behavior and school bullying victimization in children and adolescents. We also tested the mediating effects of peer alienation and student–teacher closeness, as well as the moderating effect of the educational stage.MethodsIn total, 538 children and adolescents were recruited from three suburban schools in Beijing, China (252 boys, 286 girls; mean age = 12.47; 237 elementary school students, 101 middle school students, and 200 high school students). The participants were asked to complete the measures of prosocial behavior, peer alienation, and student–teacher closeness at the initial time point and reported school bullying victimization 3 months later.ResultsWe found that prosocial behavior was directly and negatively associated with traditional bullying victimization (i.e., physical, nonphysical, and relational); however, it had no direct association with cyberbullying victimization. Prosocial behavior was indirectly associated with school bullying victimization (except in the relational dimension) via peer alienation, but no indirect effect of student–teacher closeness was found. Besides, the associations between prosocial behavior, peer alienation, student–teacher closeness, and bullying victimization were found equally among elementary, middle, and high school students.ConclusionsThe findings suggest that prosocial behavior is an important factor associated with decreased school bullying victimization, and peer relationships play a mediating role in this association. Our study extends the current understanding of prosocial behavior primarily as a consequence of child and adolescent development to an antecedent (of school bullying victimization), which contributes to a more comprehensive view of prosocial behavior.
Resting‐state coupling between HbO and Hb measured by fNIRS in autism spectrum disorder
Authors: Lin Fang , Hu Ying , Huang Weihao , Wu Xiaoyin , Sun Huiwen , Li Jun 
Published: November 2022

AbstractTo distinguish between children with autism spectrum disorder (ASD) and typically developing (TD) children, we have uncovered a new discriminative feature, hemoglobin coupling. Functional near‐infrared spectroscopy (fNIRS) was used to record resting‐state hemodynamic fluctuations in the bilateral temporal lobes in 25 children with ASD and 22 TD children, in which the coupling between low frequency oxygenated hemoglobin (HbO) and deoxygenated hemoglobin (Hb) fluctuations was evaluated by Pearson correlation coefficient. The results showed significantly weak coupling in children with ASD in both the left and right, and throughout the whole temporal cortex. To explain this observation, a simulation study was performed using a balloon model, in which we found four related parameters could impact the coupling. This study suggested that hemoglobin coupling might be applied as a new cerebral hemodynamic characteristic for ASD screening or diagnostics.
Modulation of gene expression by YTH domain family (YTHDF) proteins in human physiology and pathology
Authors: Kisan Aju , Chhabra Ravindresh 
Published: November 2022

AbstractThe advent of high throughput techniques in the past decade has significantly advanced the field of epitranscriptomics. The internal chemical modification of the target RNA at a specific site is a basic feature of epitranscriptomics and is critical for its structural stability and functional property. More than 170 modifications at the transcriptomic level have been reported so far, among which m6A methylation is one of the more conserved internal RNA modifications, abundantly found in eukaryotic mRNAs and frequently involved in enhancing the target messenger RNA's (mRNA) stability and translation. m6A modification of mRNAs is essential for multiple physiological processes including stem cell differentiation, nervous system development and gametogenesis. Any aberration in the m6A modification can often result in a pathological condition. The deregulation of m6A methylation has already been described in inflammation, viral infection, cardiovascular diseases and cancer. The m6A modification is reversible in nature and is carried out by specialized m6A proteins including writers (m6A methyltransferases) that add methyl groups and erasers (m6A demethylases) that remove methyl groups selectively. The fate of m6A‐modified mRNA is heavily reliant on the various m6A‐binding proteins (“readers”) which recognize and generate a functional signal from m6A‐modified mRNA. In this review, we discuss the role of a family of reader proteins, “YT521‐B homology domain containing family” (YTHDF) proteins, in human physiology and pathology. In addition, we critically evaluate the potential of YTHDF proteins as therapeutic targets in human diseases.
Implementation of the I‐PASS handoff program in diverse clinical environments: A multicenter prospective effectiveness implementation study
Authors: Starmer Amy J. , Spector Nancy D. , O'Toole Jennifer K. , Bismilla Zia , Calaman Sharon , Campos Maria‐Lucia , Coffey Maitreya , Destino Lauren A. , Everhart Jennifer L. , Goldstein Jenna , Graham Dionne A. , Hepps Jennifer H. , Howell Eric E. , Kuzma Nicholas , Maynard Greg , Melvin Patrice , Patel Shilpa J. , Popa Alina , Rosenbluth Glenn , Schnipper Jeffrey L. , Sectish Theodore C. , Srivastava Rajendu , West Daniel C. , Yu Clifton E. , Landrigan Christopher P. ,   the I‐PASS SHM Mentored Implementation Study Group
Published: November 2022

AbstractBackgroundHandoff miscommunications are a leading source of medical errors. Harmful medical errors decreased in pediatric academic hospitals following implementation of the I‐PASS handoff improvement program. However, implementation across specialties has not been assessed.ObjectiveTo determine if I‐PASS implementation across diverse settings would be associated with improvements in patient safety and communication.DesignProspective Type 2 Hybrid effectiveness implementation study.Settings and ParticipantsResidents from diverse specialties across 32 hospitals (12 community, 20 academic).InterventionExternal teams provided longitudinal coaching over 18 months to facilitate implementation of an enhanced I‐PASS program and monthly metric reviews.Main Outcome and MeasuresSystematic surveillance surveys assessed rates of resident‐reported adverse events. Validated direct observation tools measured verbal and written handoff quality.Results2735 resident physicians and 760 faculty champions from multiple specialties (16 internal medicine, 13 pediatric, 3 other) participated. 1942 error surveillance reports were collected. Major and minor handoff‐related reported adverse events decreased 47% following implementation, from 1.7 to 0.9 major events/person‐year (p < .05) and 17.5 to 9.3 minor events/person‐year (p < .001). Implementation was associated with increased inclusion of all five key handoff data elements in verbal (20% vs. 66%, p < .001, n = 4812) and written (10% vs. 74%, p < .001, n = 1787) handoffs, as well as increased frequency of handoffs with high quality verbal (39% vs. 81% p < .001) and written (29% vs. 78%, p < .001) patient summaries, verbal (29% vs. 78%, p < .001) and written (24% vs. 73%, p < .001) contingency plans, and verbal receiver syntheses (31% vs. 83%, p < .001). Improvement was similar across provider types (adult vs. pediatric) and settings (community vs. academic).
Poly(vinyl alcohol)/sodium alginate polymer membranes as eco‐friendly and biodegradable coatings for slow release fertilizers
Authors: Meng Wen , Zhang Xu , Zhang Yang , Zhang Xianglu , Zhu Wending , Huang Handa , Han Xiaozhao , Liu Yahua , Xu Chao 
Published: November 2022

AbstractBACKGROUNDThe use of slow release fertilizers (SRFs) is an effective approach for reducing agriculture cost, environmental and ecological issues simultaneously. The present study provides a series of poly(vinyl alcohol) (PVA)/sodium alginate (SA) polymer membranes as eco‐friendly and biodegradable coatings for SRFs. Moreover, polymer‐coated urea (PCU) granules were fabricated through coating the urea granules with the resulting membranes. Our first interest was to fabricate three membranes (PS1, PS2, PS3) of different PVA/SA weight ratios (9:1, 8:2, 7:3) using glutaraldehyde as a crosslinking agent, and crosslink the PS3 membrane with a CaCl2 solution further to obtain the PC3 membrane. The chemical properties and morphologies of the membranes were characterized. Second, the nitrogen release behavior of the PCU granules was measured and calculated, respectively.RESULTSCrosslinking with glutaraldehyde made the PS1, PS2, PS3 membranes uniform and compact, whereas crosslinking with a CaCl2 solution formed an ‘egg box’ structure inside the PC3 membrane. PS3 membrane with the minimum PVA/SA weight ratio had the highest hydrophily (water uptake: 106.25%, water contact angle: 55.1o), whereas PC3 membrane had the lowest hydrophily (water uptake: 21.57%, water contact angle: 67.3o). The biodegradation ratios of the membranes were in the range 44–60% in 90 days, indicating that they had excellent biodegradability. The measured fractional release on the day 30 of the PCU granules ranged from 89.33% to 97.07%. The calculated nitrogen release behavior agreed well with the measured values.CONCLUSIONThe resulting eco‐friendly and biodegradable PVA/SA membranes are alternative coatings for SRFs. © 2022 Society of Chemical Industry.
The mediating role of resilience in the relationship between social support and quality of life among patients after radical cystectomy: A structural equation model analysis
Authors: Yu Shuhui , Yao Xiuyu , Sang Yonghui , Yu Lingling , Shen Yiru , Che Xinyan , Ding Yanming , Huang Yanbo 
Published: November 2022

AbstractAimThis study aimed to examine the relationship between social support and quality of life in urostomy patients and identify the mediating role of resilience in that relationship.DesignA cross‐sectional design.MethodsParticipants included 232 patients who were recruited from a tertiary hospital in Beijing during March 2020 and August 2020. They completed questionnaires about perceived social support, resilience and ostomy‐related quality of life. Structural equation modelling was performed to analyse the data.ResultsThe mean age of patients was 65.79 (SD = 8.67) years, and the mean length of time after surgery was 42.14 (SD = 15.76) months. Urostomy patients' quality of life, social support and resilience were all above moderate. Social support had a positive direct effect on the quality of life and a positive indirect effect on the quality of life through the mediating role of resilience.
An Early Holocene case of congenital syphilis in South America
Authors: Oliveira Rodrigo , Strauss André , Murrieta Rui , Castro Claudio , Matioli Antônio 
Published: November 2022

AbstractOver millennia, treponematoses have been present in the American continent. The search for the oldest case and the description of the pathological characteristics are fundamental to understanding the evolutionary history of treponemal diseases, as well as the evolution of the humans who contracted them. A child with a minimum age of 9400 years before present exhumed at “Lapa do Santo” cave, Brazil, presents characteristic lesions of congenital syphilis. This well‐preserved skeleton with dental and bone pathological evidence is the oldest syphilitic case of America and reinforces the presence of syphilis, at least 10,000 years ago, in the continent.
Hyperostosis frontalis interna: A new perspective in burned individuals' analysis for determining age and sex from archeo‐anthropological and medicolegal contexts
Authors: Du Fayet de la Tour Anaïs , Couture Christine , Paraf François , Duday Henri 
Published: November 2022

AbstractHyperostosis frontalis interna (HFI) is a benign bone lesion, which is asymptomatic for the most part and is manifested by the accretion of bone on the endocranial surface of the frontal bone.This type of lesion has been discussed in a multitude of publications since the 19th century. Although a consensus has yet to be reached as to its etiology, it would seem generally accepted that hormones play a role, which would explain the high frequency of this disorder among older and postmenopausal women.The objective of this study is to demonstrate the benefit of HFI diagnosis for identifying individuals (estimating their age and determining their sex) in the archeo‐anthropological analysis of ancient cremation burial sites and in medicolegal investigations on burned human remains.Our sample consisted of 82 cremated individuals from the Roman Porta Nocera necropolis in Pompeii (1st century BC–1st century AD). After excluding juveniles under 15 years of age, as HFI primarily affects adult individuals, our sample was reduced to 71 individuals, and the prevalence of HFI was 15.5% (n = 11). A statistically significant difference is observed between women and men, and no individuals previously identified as male displayed HFI. Furthermore, 90.9% of the individuals displayed degenerative conditions in conjunction with HFI, showing a highly significant correlation of this lesion with signs of aging (chi‐square 9.365; p = 0.01). Finally, this disorder boosted diagnostic performance in respect of determining sex, increasing the number of biologically sexed individuals from 46.5% to 53.5%. Therefore, we propose that the HFI diagnosis offers genuine added value in the identification of burned human remains.