bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying Patients with Epilepsy Having Depression/Anxiety Disorder using Common Spatial Patterns of Functional EEG Networks
Authors: Zhang Yuanyuan , Du Kejun , Qu Zhichuang , Lin Yuhang , Guo Daqing , Chen Xin , Shu Haifeng , Yu Sixun 
Published: April 2022

Abstract
Background
The presentation of epilepsy with depression/anxiety disorder (E-AD) is a complex comorbidity, and a systematic psychiatric screen poses a heavy economic burden for the patients. Traditionally, distinguishing between E-AD patients and epilepsy without depression/anxiety disorder (E-no-AD) needs multiple evaluations, which is incredibly difficult owing to the high costs. Therefore, there is an urgent need for a reliable assessment protocol to initially distinguish between the two types of patients.
Results
Based on the resting-state electroencephalography signals collected from E-AD and E-no-AD patients, the classification performances were estimated for three different network features, using the support vector machine. The results revealed that the SPN feature of functional network connectivity was remarkably superior to the traditional network feature for distinguishing between the two groups of patients, with appreciable classification accuracy values of 89.90% and 87.37% sensitivity and 91.67% specificity.
Conclusions
These findings demonstrate the superiority of functional SPN features as a reliable way in characterizing the differences of E-AD and E-no-AD patients and potentially provide insights for the complex mechanisms causing E-AD in patients.
Contribution of low population immunity to the severe Omicron BA.2 outbreak in Hong Kong
Authors: Chen Lin-Lei , Abdullah Syed Muhammad Umer , Chan Wan-Mui , Chan Brian Pui-Chun , Ip Jonathan Daniel , Chu Allen Wing Ho , Lu Lu , Zhang Xiaojuan , Zhao Yan , Chuang Vivien Wai-Man , Au Albert Ka-Wing , Cheng Vincent Chi-Chung , Srid Siddharth , Yuen Kwok-Yung , Hung Ivan Fan Ngai , Chan Kwok-Hung , To Kelvin Kai-Wang 
Published: April 2022

Abstract
Monitoring population protective immunity against SARS-CoV-2 variants is critical for risk assessment. In this serosurveillance study, older adults show much lower seropositive rates of neutralizing antibody (NAb) against ancestral virus than the younger population. The increase in NAb seopositive rate generally follows the population vaccination uptake rate, but older adults have a much lower NAb seropositive rate than vaccination uptake rate. For all age groups, the seropositive rates of NAb against Omicron variant are much lower than those against the ancestral virus. During the fifth wave of COVID-19 in Hong Kong which is dominated by Omicron sublineage BA.2, the case-fatality rate is exceptionally high in the ≥80 year-old age group (9.2%). Our study suggests that the severe BA.2 outbreak in Hong Kong can be attributed by the lack of protective immunity in the population, especially among the vulnerable older adults, and highlights the importance of continual surveillance of protective immunity against emerging variants of SARS-CoV-2.
Fully inkjet-printed flexible organic voltage inverters as a basic component in digital NOT gates
Authors: Luczak Adam , Mitra Kalyan Y. , Baumann Reinhard R. , Zichner Ralf , Luszczynska Beata , Jung Jaroslaw 
Published: April 2022

Abstract
In relation to conventional vacuum-based processing techniques inkjet printing enables upscaling fabrication of basic electronic elements, such as transistors and diodes. We present the fully inkjet printed flexible electronic circuits, including organic voltage inverter which can work as a NOT logic gate. For this purpose the special ink compositions were formulated to preparation of gate dielectric layer containing poly (4-vinylphenol) and of the semiconductor layer poly[2,5-(2-octyldodecyl)-3,6-diketopyrrolopyrrole-alt-5,5-(2,5-di(thien-2-yl)thieno [3,2-b]thiophene)] (DPPDTT). A printed photoxidized poly (3-hexyltiophene) semiconductor was used as the active layer of the resistors. The operation of the printed inverters and NOT logic gates was analyzed based on the DC current-voltage characteristics of the devices. The resistance of the devices to atmospheric air was also tested. Not encapsulated samples stored for three years under ambient conditions. Followed by annealing to remove moisture showed unchanged electrical parameters in comparison to freshly printed samples.
A clinical and biomechanical comparison of INFIX plus single versus double sacroiliac screw fixation for unstable pelvic ring injury
Authors: Chen Hongfen , Ding Chao , Liu Yongqiang , Huang Feng , Li Heng , Guo Qingxiang , Yang Yuehua , Zhong Hua , Yang Shaozheng 
Published: April 2022

Abstract
Background: The aim of this study is to compare the clinical and biomechanical outcome of INFIX plus single with two sacroiliac screw ﬁxation for unstable pelvic fractures of Type C.Methods: Sixteen cadavers were randomly subjected to INFIX plus single or double sacroiliac screw ﬁxations, and then mounted onto the Electroforce loading machine under different vertical load. To investigate the clinical outcomes of the two techniques, Nineteen patients were retrospectively analyzed. The main outcome measures were postoperative radiographic reduction grading (using the Tornetta and Matta grading system), functional outcome (using the Majeed scoring system), and incidence of complications. Results: In the biomechanical study, INFIX plus double sacroiliac screw ﬁxation showed better biomechanical stability than fixation with a single sacroiliac screw (p < 0.05). In our clinical case-series, all 19 patients had bony union 6 months after the operation. INFIX plus double sacroiliac screw ﬁxation also demonstrated a better functional outcome and a higher radiographic satisfactory rate than INFIX plus single sacroiliac screw ﬁxation (79.25±5.47; 91.33±4.97; p<0.05), ( 77.78% vs. 60%; p = 0.05). One patient in INFIX plus single screw ﬁxation group had screw loosening at follow-up 6 months postoperatively. One case in each group suffered heterotopic ossification and the lateral femoral cutaneous nerve paralysis, and one patient suffered from infection.Conclusion: INFIX plus double sacroiliac screw ﬁxation demonstrated more stability in cadaveric biomechanical analysis and better clinical outcomes than INFIX plus single sacroiliac screw ﬁxation.
Deletion of Bak1 alleviates microglial necroptosis and neuroinflammation after experimental subarachnoid hemorrhage
Authors: Peng Jianhua , Qiu Xiancheng , Tao Qianke , Zhang Lihan , Kuang Chenghao , Xie Yuke , Zhang Lifang , Yin Shigang , Jiang Yong 
Published: April 2022

Abstract
Background: Microglial necroptosis exacerbates neurodegenerative diseases, central nervous system injury and demonstrates a pro-inflammatory process, but its contribution to subarachnoid hemorrhage (SAH) is poorly characterized. BCL-2 homologous antagonist-killer protein (Bak1), a critical regulatory molecule of endogenous apoptosis, can be involved in the pathological process of necroptosis by regulating mitochondrial permeability. Despite its possible significance in microglia necroptosis-mediated inflammation, few studies have investigated Bak1's role in microglia necroptosis regulation.Methods: In vivo, an experimental subarachnoid hemorrhage in mice identified microglia necroptosis and adeno-associated virus targeted interference with microglia Bak1 to determine its effect on neuroinflammation. In vitro knockout of Bak1 in BV2 microglia identified its regulatory role in microglia necroptosis and neuroinflammation, and RNA-Seq clarified the potential downstream regulatory mechanisms.Results: Microglia undergo necroptosis after subarachnoid hemorrhage in vivo and vitro. Knocked-down of Bak1 by adeno-associated virus attenuates microglial necroptosis, alleviates neuroinflammation, and improves neurological function after SAH. Increasing Bak1 expression and mediating BV2 microglia pro-inflammatory phenotype transformation, exacerbating oxidative stress and neuroinflammation. Abrogating Bak1 reduces necroptosis by downregulating the expression of phosphorylated pseudokinase mixed lineage kinase domain-like protein (p-MLKL), then downregulating pro-inflammatory phenotype gene expression. RNA-Seq shows that disrupting BV2 Bak1 downregulates multiple immune and inflammatory pathways and ameliorates cell injury by elevating Thrombospondin 1 (THBS1) expression.Conclusions: Our finding demonstrated that deletion of bak1 alleviates microglial necroptosis, neuroinflammation and improved neurological functions after SAH, which was, at least in part, mediated by activation of THBS1 signaling pathway. Bak1 may be a potential therapeutic strategy for the management of SAH.
Bioinformatics analysis of differentially expressed genes in human embryonic stem cells and their differentiated dorsal root ganglion
Authors: Liu Kuang-pin , Ma Wei , Yang Jinwei , Zhang Si-jia , Liu Wei , Zhu Kewei , Liu Jie , Wu Hongjie , Li Liyan 
Published: April 2022

Abstract
To explore the difference of gene expression between undifferentiated hESCs and DRG induced by hESCs differentiation for 1 day by bioinformatics technology, and find out the key differential genes and their potential molecular regulation mechanism; The functions involved are analyzed and predicted. Download the gene chip data series GSE75582 related to stem cells and dorsal root ganglion from the geo expression database, import the gene chip online analysis tool GEO2R, screen out the differential genes, and construct the volcano map. Webgestalt database was used for GO function analysis and KEGG pathway enrichment analysis, and string analysis and Cytoscape software were used to construct PPI network. Results 4102 differentially expressed genes were screened, of which 2674 were up-regulated and 1428 were down regulated. Bioinformatics analysis of differentially expressed genes showed that the genes were mainly enriched in axon guidance, signaling pathways regulating pluripotency of stem cells, pathways in cancer, ECM receiver interaction, focal adhesion, cell adhesion molecules (CAMs), PI3K Akt signaling pathway, MAPK signaling pathway, TGF beta signaling pathway Ras signaling pathway, which extracts differential genes in stem cell related signaling pathways, including 34 up-regulated genes and 13 down regulated genes. Conclusion BMP4, Sox2, FGF2, Nanog, Smad3, KLF4, gsk3b and FGFR2 in undifferentiated hESCs are closely related to stem cell self-renewal; BMP4, Sox2, FGF2, Smad3, gsk3b and FGFR2 were mostly related to neuropathic pain and neurite growth. However, the functions of these key genes need to be further confirmed by future experimental studies.
The clinical course of herpes zoster is similar in immunocompetent and immunocompromised pediatric patients
Authors: Bieńkowski Carlo , Talarek Ewa , Pokorska-Śpiewak Maria 
Published: April 2022

Abstract
Herpes zoster (HZ) is an infectious disease caused by reactivation of latent varicella-zoster virus infection. It manifests with vesicular eruption in an affected dermatome. We aimed to compare clinical manifestation of herpes zoster in 152 immunocompetent and immunocompromised children. Clinical manifestations were similar. Serious complications, although uncommon, affected not only immunocompromised patients but also otherwise healthy children.
Trans-catheter closure of ASD and abnormal connection of left pulmonary vein to vertical vein: Follow-Up report
Authors: Shabestari Mahmoud Mohamadzadeh , Kerachian Abdollah , Poorzand Hoorak , Eshraghi Ali , Keihanian Faeze 
Published: April 2022

Abstract
Background: Partial anomalous pulmonary venous connection (PAPVC) is a rare congenital anomaly in which one or more pulmonary veins are connected to the venous circulation leading to left to right heart shunt. Although correction of anomalous pulmonary venous connection is achieved through surgery, there are rare instances where the abnormal pulmonary vein has dual connection to both left atrium and the major systemic veins. Under these circumstances catheter-based treatment might become a feasible option. Case presentation: We report a case of atrial septal defect (ASD) and dual drainage of left upper pulmonary vein, which were successfully addressed by occluder devices and followed carefully while detecting early clot formation behind the implanted device in the vertical vein.Conclusion: For comprehensive evaluation of patients with ASD, assessment of pulmonic veins is crucial and in the presence of a vertical vein, the dual drainage of pulmonic veins should be considered.
SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer
Authors: deng kui , zhang zhen , shang hongkai , xu qiaoping 
Published: April 2022

Abstract
Ovarian cancer is a disease with increasing incidence worldwide, and there is an urgent need for chemotherapy and biological targeted therapy.Epithelial-mesenchymal transformation (EMT) is an important initiation stage for tumor cells to acquire the ability to invade and metastasize.A growing number of findings suggest that human Schlafen family member 5(SLFN5) plays a key role in malignancy.However, the role of SLFN5 in ovarian cancer cells has not been fully elucidated.Samples were collected from patients with ovarian cancer diagnosed in Hangzhou First People's Hospital, and the expression of SLFN5 was detected by fluorescence quantitative PCR.The relationship between SLFN5 expression and the progression and malignancy of ovarian cancer was analyzed by using the expression profile data from the Cancer Genome Atlas (TCGA) database.The mRNA expression levels of SLFN5 related upstream and downstream signaling pathways were studied by fluorescence quantitative PCR.Silencing SLFN5 was performed by siRNA transfection.The expression of SLFN5 and transfer-related proteins was examined by Western blot.Transwell and wound healing experiments investigated the migration and invasion ability of ovarian cancer cells. TCGA database analysis results showed that in the population with high SLFN5 expression,compared with the group with low SLFN5 expression,OS was worse (P=0.011).SLFN5 silencing had a significant inhibitory effect on EMT and invasion movement of ovarian cancer cells.RT-PCR method was used to detect the mRNA changes of SLFN5 in ovarian cancer tissue and adjacent tissue. It was found that the expression of SLFN5 in ovarian cancer tissue was increased, with a significant difference (P<0.05). Together, these results suggest that SLFN5 may play a synergistic role in tumorigenesis and development of ovarian cancer cells, providing a potential target for future drug development for the treatment of ovarian cancer.
Diagnosis of atherosclerosis based on pulse wave and ensemble learning method: Weighted-Ensemble model
Authors: Chen Rongbin , Liu Wenjun , Huang Hui , Bai Songtao 
Published: April 2022

Abstract
Purpose: Atherosclerosis (AS) is closely related to cardiovascular disease (CVD). Nowadays, many scholars have conducted research on CVD, but the diagnosis of AS can only be diagnosed based on traditional medical methods. Pulse wave velocity (PWV) can evaluate potential AS. As a new technology, ultrafast pulse wave velocity (ufPWV) can accurately evaluate PWV. This research aims to screen out relevant features through feature engineering methods and build a Weighted-Ensemble model based on these features to predict AS, which can assist doctors in making more effective diagnosis of atherosclerosis. Methods: In this paper, the traditional statistical analysis method and Random forests (RF) are used to ensemble selection characteristics. This paper improves the ensemble model and applies it to the prediction of AS. Based on the idea of bagging, the base model of RF is changed, and all decision trees in RF are replaced with prediction models with better generalization ability. In order to make full use of models with high generalization ability, this study will also introduce a boosting strategy to weight each base model to form a Weighted-Ensemble model with high generalization ability. The accuracy (ACC), sensitivity (TPR), specificity (TNR) and AUC are four evaluation criteria to evaluate the model.Results: There are 37 features in the data set. Based on the statistical analysis of the data set, a total of 16 characteristics affect the diagnosis of AS. According to the importance of the features obtained by RF model, the 10 most important features are used to construct a Weighted-Ensemble model. The results show that the ACC, TPR, TNR and AUC of the Weighted-Ensemble model are 0.91, 0.93, 0.89 and 0.91 respectively. Compared with each model, the ACC, TPR and AUC of the Weighted-Ensemble model are better than other models. The analysis shows that the Weighted-Ensemble model is superior to traditional machine learning methods in distinguishing patients as normal or atherosclerosis. Conclusions: The accuracy of the algorithm based on the Weighted-Ensemble model in predicting AS has been confirmed in this paper, which implicates that the Weighted-Ensemble model can be successfully used in the atherosclerosis diagnosis and decision-making system.
Hindlimb unloading induces time-dependent disruption of testicular histology in mice
Authors: Ranade Anu V , Qaisar Rizwan , Karim Asima , Azeem Muhammad , Jose Josemin , Ramachandran Gopika , Ibrahim Zainab , Elmoselhi Adel , Ahmad Firdos , Hassan Wael A Rahman 
Published: April 2022

Abstract
Objectives: Mechanical unloading of the body in the hindlimb unloaded (HU) mice induces pathology in multiple organs, but the effects on testes are poorly characterized. We investigated the histology and Raman spectroscopy of the mouse testes following HU condition. Methods: We divided male, c57BL/6j mice into ground-based controls or experimental groups for two and four weeks of HU. The testes tissues were dissected after euthanasia to investigate histological and Raman spectroscopic analysis.Results: We found an HU-induced atrophy of testes irrespective of the time duration (p < 0.05). Histological analysis revealed that the HU induced epithelial thinning, luminal widening, and spermatozoa decline in the seminiferous tubules of the mouse testes. In addition, we found a thickening of the epididymal epithelia and tunica albuginea. These changes were accompanied by a generalized depression in the Raman spectra, indicating an altered concentration and/or orientation of several molecules. We also report reduced signal intensities of hydroxyproline and tryptophan, potentially contributing to testicular pathology during HU. Conclusion: Taken together, our findings indicate that the two or four weeks of HU induce disruption of testicular architecture and molecular phenotypes. Our results may have implications for understanding and/or treating male infertility associated with prolonged bed rest and spaceflight.
Identification and validation of a novel LncRNA TRG-AS1 that inhibits colorectal cancer proliferation and glycolysis
Authors: Li Lu , Zhang Yong , Li Kunkun , Xing Mengxuan , Xiao Xingguo , Zhang Li , Chu Feifei , Wu Huili 
Published: April 2022

Abstract
Abnormal glycolytic metabolism is one of the hallmarks during progression of malignant human colorectal cancer. Abundant evidence has illustrated that long non-coding RNA participated in glycolytic metabolism of various tumors. However, the lncRNA involved in the inhibition of glycolysis in CRC is largely unknown. In this study, we identified the lncRNA TRG-AS1 that inhibits CRC glycolysis in CRC by bioinformatic analysis, and its biological function was investigated in vitro and in vivo. LncRNA TRG-AS1 was identified and analyzed in The Cancer Genome Atlas database and Gene Expression Omnibus databases, and further confirmed in CRC tissues and adjacent normal tissues with qRT-PCR. TRG-AS1 overexpressing stable cell line and silence the target gene cell line were generated. Glucose assay kits and Pyruvate assay kits were utilized to analyze glycolysis. The cell proliferation ability was evaluated by CCK8, Colony formation assays and EdU. Xenograft tumor experiments were performed to assess proliferative capacity. Cell migration and invasion abilities were examined using the Transwell assay. TRG-AS1 was remarkably decreased in CRC tissues and cell lines. Higher TRG-AS1 expression is remarkably associated with more prolonged survival time. Over-expression of TRG-AS1 suppressed glycolysis, cell proliferation, invasive and migration abilities of CRC cells. On the contrary, TRG-AS1 silence attenuated CRC cells glycolysis, promoted cell proliferation, migration and invasion. Collectively, the results demonstrate that TRG-AS1 functioned as a tumor suppressor to contribute to CRC progression, is a promising prognostic factor. Our study provided a novel insight into the pathogenesis of CRC and a promising therapeutic target for CRC treatment.
The efficacy and safety of regional citrate anticoagulation centrifugal plasma exchange is equivalent to those of systemic heparin anticoagulation membrane plasma exchange in patients with liver failure: a single center retrospective cohort study
Authors: Zhu Xin-Fang , Li Jia-Qiang , Liu Tian-Tian , Wang Yuan , Zhong Yao , Gao Qin-Mei , Zhang Qi , Yu Kang-Kang , Huang Chong , Li Ning , Lu Qin , Zhang Wen-Hong , Zhang Ji-Ming , Xia Rong , Zheng Jian-Ming 
Published: April 2022

Abstract
Background: The purpose of this study is to compare with the efficacy and safety of regional citrate anticoagulation centrifugal plasma exchange and systemic heparin anticoagulation membrane plasma exchange in patients with liver failure.Methods: This study was a single center and retrospective study. All enrolled liver failure patients were treated with therapeutic plasma exchange. The patients treated by regional citrate anticoagulation centrifugal plasma exchange were defined as RCA group and those treated by systemic heparin anticoagulation membrane plasma exchange were defined as heparin group, respectively. Survival and clinical characteristics, including patients’ age, gender, total bilirubin, serum creatinine, international normalized ratio, platelet count, types of liver failure, arterial blood gas analysis, the model for end-stage liver disease (MELD) score, and occurrence of complications were compared between the two groups. Survival analyses of two groups and subgroups classified by MELD score were performed by Kaplan-Meier method and were compared by the log-rank test.Results: There were 69 patients enrolled in this study, 51 patients in RCA group and 18 patients in heparin group respectively. In overall patients, the 28-day mortality rate was 41% in RCA group and 39% in heparin group (P > 0.05). The 90-day mortality rate was 59% in RCA group and 50% in heparin group (P > 0.05). MELD best-fit value is 30, and area under the ROC curve is 0.7208 in the transplantation free patients with liver failure in both RCA group and heparin group. There was no significant difference in the occurrence of complications between the two groups.Conclusions: In conclusion, our study demonstrates that the efficacy and safety of regional citrate anticoagulation centrifugal plasma exchange is equivalent to those of systemic heparin anticoagulation membrane plasma exchange in patients with liver failure. RCA plasma exchange at an interval of 48 hours in patients with acute or acute-on-chronic liver failure may be an effective in reducing the occurrence of the complication of citrate accumulation. RCA centrifugal plasma exchange treatment can achieve a good prognosis and well tolerance, especially in MELD<30 patients with liver failure.