bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalencia de factores psicosociales de riesgo cardiaco en cardiópatas en rehabilitación
Authors: Valls Esperanza 
Published: April 2022

La promoción de programas multidisciplinares de prevención y los avances en intervención cardíaca han mejorado el pronóstico de la enfermedad cardiovascular (ECV) aunque sigue siendo la principal causa de muerte en adultos de mediana edad. Los programas de rehabilitación cardíaca (PRC) inciden en modificar los posibles factores de riesgo cardíaco (FRC) implicados, incluidos los predisponentes psicosociales (emocionales, de personalidad y comportamentales) que son el objetivo prioritario de las intervenciones psicológicas inmersas en ellos. El objetivo del presente estudio es analizar la prevalencia de dichos factores psicosociales en una muestra española (N: 905; 82% varones), incluida en un PRC y detectar la diferente prevalencia según el género, el riesgo cardíaco y la edad.
Resultados: prevalencia en depresión: 29%, afecto negativo: 33,4%, expresión de ira: 30,4% y ansiedad 20%. El 17,3% presentó personalidad tipo-A (PCTA) y obtuvo una reducida calidad de vida el 25,3% y bajo apoyo social el 12,5%. Las mujeres mostraron mayor psicopatología emocional y menor uso de estrategias activas y PCTA. Además, con mayor riesgo cardiológico se obtuvo un perfil psicológico más adverso mientras que, según la edad, los más jóvenes presentaron mayor prevalencia de factores psicosociales. Conclusión: por su elevada prevalencia, se hace necesario enfatizar la atención a los factores psicosociales en los programas de rehabilitación y hacerlo diferencialmente conforme los perfiles de los usuarios.
Enfermedad renal crónica avanzada: ansiedad, depresión y percepción de amenaza
Authors: Lacomba-Trejo Laura , Mateu-Mollà Joaquín , Cabajo Álvarez Eva , Oltra Benavent Ana María , Galán Serrano Antonio 
Published: April 2022

Marco teórico: la percepción de su enfermedad que tengan los pacientes con enfermedad renal crónica avanzada (ERCA) puede generar sintomatología depresiva y ansiosa. El objetivo general fue valorar la sintomatología ansiosa y depresiva y la percepción de amenaza de la enfermedad de pacientes con ERCA.
Método: 70 pacientes (80% barones), de entre 38-88 años (M=68,88; DT= 9,98) fueron evaluados mediante: la Escala Hospitalaria de Ansiedad y Depresión y la de Percepción de amenaza de la enfermedad. Se realizaron análisis descriptivos, pruebas t, dde Cohen y correlaciones de Pearson.
Resultados: un 41,79% presentaba sintomatología ansiosa y un 25,38% depresiva, percibían su enfermedad como moderadamente negativa. La sintomatología emocional fue mayor en las mujeres y se asoció con la percepción de amenaza de la enfermedad.
Conclusiones: es necesario conocer los factores de riesgo para desarrollar programas de intervención que incidan en ellos y favorezcan la salud física y mental del paciente.

Palabras clave: Enfermedad Renal Crónica Avanzada, Ansiedad, Depresión, Percepción de Amenaza de la Enfermedad, Factores de riesgo
Association between Health-Related Quality of Life and Coping Strategies of Gastrointestinal Cancer Caregivers: A Rasch Analysis
Authors: Ikhsan Nur Afrina , Mahmud Zamalia , Abdullah Nik Nairan 
Published: April 2022

Abstract
The number of gastrointestinal (GI) cancer patients in Malaysia is rising. Caregivers need to perform their responsibilities as they spend most of their time providing care for the patient in their homes. To what extend does health-related quality of life (HRQOL) of GI caregivers relates to coping strategies of caregivers are unknown and yet to be investigated. This study investigates the caregivers’ response pattern towards HRQOL and coping strategies survey items across several domains and the association between HRQOL and coping strategies of the caregivers. This study used Rasch measurement model in the analysis of survey data gathered from 323 GI cancer caregivers from three public hospitals. Data were collected using HRQOL and Coping strategies instruments administered to the GI caregivers. It was found that four items (QOL_9, QOL_20, QOL_25 and QOL_31) under the Burden domain are among the most difficult to endorse by GI caregivers in the HRQOL items. Meanwhile, two items (COPE4 and COPE11) under the Substance Use domain in the coping strategies constructs are most difficult to endorse by GI cancer caregivers. This study also discovered that majority of caregivers who have issues with mental strain, disruptiveness, daily focus and sleep disruption applied positivity adaptation and use of emotional support as their coping strategies.
The Pillars of Health: Influence of Multiple Lifestyle Behaviors on Body Mass Index and Depressive Symptoms in Adult Twins
Authors: Duncan Glen E. , Avery Ally R. , Tsang Siny , Watson Nathaniel F. , Williams Bethany , Turkheimer Eric 
Published: April 2022

Abstract
Background: Guidelines promoting healthy lifestyles are cornerstones of chronic disease prevention and treatment. The purpose of this study is to investigate independent and joint associations of five key health behaviors with health outcomes (body mass index (BMI kg/m2) and depressive symptoms) in adult twins. Methods: We included 6,048 twin pairs from a community-based registry. Five key health behaviors were: (1) ≥ 8 hours of sleep per night, (2) ≥ 5 servings of fruits and vegetables daily, (3) ≤ 2 hours sedentary time per day, (4) ≥ 150 min of moderate-to-vigorous physical activity (MVPA) per week, and (5) no smoking. We analyzed phenotypic associations between behaviors and outcomes; whether phenotypic associations were confounded by additive genetic and shared environmental factors within twin pairs (“quasi-causal” associations); and which behaviors, considered simultaneously, had the largest associations with outcomes. Results: We found negative phenotypic associations between number of behaviors achieved with BMI and depressive symptoms score (ps < 0.05). Associations remained significant, though attenuated, when controlling for genetic and shared environmental factors, and demographics, for depressive symptoms score but not BMI (p < 0.05). Quantitative variable importance measures derived from regression tree models showed sedentary time and MVPA were the most important variables in partitioning twins with different BMI, and smoking and sedentary time for partitioning twins with different depressive symptoms score. Conclusions: Achievement of commonly endorsed health behaviors is associated with lower BMI (especially sedentary and MVPA targets) and depressive symptoms score (especially sedentary and smoking targets). This provides further support of health behavior promotion to improve health outcomes.
iEnhancer-DCLA: Using the original sequence to identify enhancers and their strength based on a deep learning framework
Authors: Liao Meng , Zhao Jian-ping , Tian Jing , Zheng Chun-Hou 
Published: April 2022

Abstract
Enhancers are small regions of DNA that bind to proteins, which enhance the transcription of genes. The enhancer may be located upstream or downstream of the gene. It is not necessarily close to the gene to be acted on, because the entanglement structure of chromatin allows the positions far apart in the sequence to have the opportunity to contact each other. Therefore, identifying enhancers and their strength is a complex and challenging task. In this article, a new prediction method based on deep learning is proposed to identify enhancers and enhancer strength, called iEnhancer-DCLA. Firstly, we use word2vec to convert k-mers into number vectors to construct an input matrix. Secondly, we use convolutional neural network and bidirectional long short-term memory network to extract sequence features, and finally use the attention mechanism to extract relatively important features. In the task of predicting enhancers and their strengths, this method has improved to a certain extent in most evaluation indexes. In summary, we believe that this method provides new ideas in the analysis of enhancers.
Rhizonium alarensis sp. nov., and Rhizonium halophilensis sp. nov., isolated from the nodule and rhizosphere of Lotus japonicus
Authors: Wan Chuanxing , Shen Hongling , Luo Xiaoxia , Xia Zhanfeng 
Published: April 2022

Abstract
Two strains (TRM95111T and TRM95001T) of Gram-stain-negative, aerobic, rod-shaped mycrobacteria were isolated from the nodule and rhizosphere of Lotus japonicus grown in the campus of Tarim University in Alar, Xinjiang, China. Strain TRM95111T and strain TRM95001T shared 93.1% 16S rRNA gene sequences similarity with each other and had 98.2% and 97.9% 16S rRNA gene sequence similarity to the closest species Rhizobium subbaraonis JC85T and Rhizobium halotolerans AB21T, respectively. Phylogenetic analyses based on 16S rRNA gene sequences, two housekeeping gene sequences (recA, atpD) and core genes showed that two strains belonged to the genus Rhizobium. The values of digital DNA-DNA hybridization (dDDH) between strain TRM95111T and the closest strains R. subbaraonis JC85T and R. azooxidifex Po 20/26T were 21.8% and 21.4%, respectively. The dDDH values between strain TRM95001T and the closest strains R. halotolerans AB21T and R. flavum YW14T were 22.6% and 26.7%, respectively. Whole genome average nucleotide identity (ANI) values of the strain TRM95111T were 75.6%-77.5% and strain TRM95001T were 79.2%-79.6%, compared to their closely related strains. The G + C content values of strain TRM95111T and TRM95001T were 65.1 mol% and 60.7 mol%, respectively. Two isolates contained predominant quinone of Q-10 and the major fatty acids was C18:1ω7c. The polar lipids of strain TRM95111T included unidentified aminophospho lipids (APL1-3), unidentified phospholipids (PL1-2), phosphatidylcholine (PC), unidentified lipids (L1-5) and phospholipids of phospholipids of unknown structure containing glucosamine (NPG), compared to the polar lipids of strain TRM95001T including unidentified aminophospho lipids (APL1-3), unidentified phospholipids (PL1-2), phosphatidylcholine (PC), unidentified lipids (L2-5), hydroxy phosphatidy ethanolamine (OH-PE) and phospholipids of phospholipids of unknown structure containing glucosamine (NPG). Nodulation tests showed that two strains could induce nodules formation in L. japonicus. Based on genomic, phenotypic and phylogenetic analyses, strain TRM95111T and strain TRM95001T are suggested to represent two new species of the genus Rhizobium, whose names are proposed as Rhizobium alarensis sp. nov. and Rhizobium halophilensis sp. nov. The type strains are TRM95111T (= CCTCC AB 2021116T = JCM34826T) and TRM950011T (= CCTCC AB 2021095T = JCM34967T), respectively.
A novel nitidine chloride nanoparticle overcomes the stemness of CD133 + EPCAM + Huh7 Hepatocellular Carcinoma Cells for liver cancer therapy
Authors: Li Danni , Zhang Qiying , Zhou Yuzhu , Zhu Hua , Li Tong , Du Fangkai 
Published: April 2022

Abstract
Background: Stemness of CD133+EPCAM+ hepatocellular carcinoma cells ensures cancer resistance to apoptosis ,which is a challenge to current liver cancer treatments. Here we discovered that a novel nitidine chloride nanoparticle (TPGS-FA/NC, TPGS-FA: folic acid modified D-α-tocopheryl polyethylene glycol 1000 succinate , NC: nitidine chloride) targeted Huh7 human hepatocellular carcinoma and promoted its apoptosis in mice and cells. CD133 expression regulates AQP3 expression, promoting to increase the stemness properties of hepatoma cells. Importantly, AQP3 is association with stimulation and nuclear translocation of STAT3 with a increasing expression level of CD133 . Methods: Cell viability was assessed by MTT and colony assays.TPGS-FA/NC nanoparticles were assayed by using confocal microscopy targeting Huh7 Hepatocellular Carcinoma Cells . A sphere culture technique was used to enrich cancer stem cells (CSC) and sort the CD133+EPCAM+ Huh 7 cells by magnetic-activated cell sorting assay. The proteins were examined by immunohistochemistry and western blotting assay. Result: TPGS-FA/NC nanoparticles reduced the CD133+EPCAM+ Huh7 cells numbers in vitro. Furthermore, its time-dependently suppressed the AQP3/CD133/STAT3/JAK signaling pathways. Nitidine chloride nanoparticles therapy prevented and treated hepatocellular carcinoma in mice without adverse effects. Conclusions: TPGS-FA/NC is shown to a promising and safe drug against liver cancer therapy via AQP3/STAT3/CD133 axis.
In Vivo CRISPR Screens Identify RhoV as a Pro-Metastasis Factor of Triple-Negative Breast Cancer
Authors: Shao Zhi-Ming , Jin Ming-Liang , Gong Yue , Ji Peng , Hu Xin 
Published: April 2022

Abstract
Background: Metastasis is the main death reason for triple-negative breast cancer (TNBC). Thus, identifying the driving genes associated with metastasis of TNBC is an urgent need. CRISPR screens have dramatically enhanced genome editing and made it possible to identify those genes associated with tumor metastasis. Methods: Here, we performed customized in vivo CRISPR screens targeting metastasis-related genes obtained from transcriptome analysis. By applying Cox proportional hazard regression models, we developed and verified a three-gene signature (RhoV, TSNARE1 and C19orf33) to predict the disease-free survival in TNBC. Next, immunoprecipitation and LC-MS/MS were conducted to explore the metastasis mechanism of RhoV.Results: The three-gene signature successfully predicts the disease-free survival in TNBC. Furthermore, our data demonstrated RhoV as a key gene involving in tumor metastasis, which was frequently upregulated in TNBC and correlated with poor survival. Knockdown of RhoV significantly suppressed cell invasion, migration and metastasis both in vitro and in vivo. In addition, we explored the physically association between RhoV and activated EGFR and verified the association was dependent on GRB2 through a specific proline-rich motif in N-terminus of RhoV. Conclusions: Taken together, our research firstly reveals that RhoV serves as an EGFR-mediated metastasis mechanism activator and thus provides a mechanistic basis for TNBC metastasis and may be a valuable therapeutic target.
Comparative proteomic analysis of mild and severe endometritis determined by LC-MS/MS
Authors: Jiang Xingcan , Li Ziyuan , Huang Cong , Qiu Rendong , Wang Aihua , Lin Pengfei , Tang Keqiong , Chen Huatao , Zhou Dong , Jin Yaping 
Published: April 2022

Abstract
Xinong Saanen goats are among the most common dairy goats in China and serve as major sources of milk in the Chinese dairy industry. Escherichia coli is a Gram-negative bacterium frequently implicated in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria and is involved in pathogenic processes that lead to post-partum metritis and endometritis in dairy goats. After binding to toll-like receptor 4, LPS induces reactions in the endometrium, leading to acute or chronic inflammation, which impairs reproductive function. The purpose of this study was to evaluate the effects of LPS on goat endometrial epithelial cells (gEECs) and the changes that occur in response to LPS. The proteomic map of gEECs exposed to different concentrations of LPS was studied using unlabeled liquid chromatography with tandem mass spectrometry. A total of 1178 proteins were identified in all samples, and 13 differentially expressed proteins were subjected to quantitative and gene ontology functional annotation analyses. In conclusion, this study shows that specific proteins expressed in the goat uterus are altered according to the concentration of LPS.
FBPP: Software to design PCR primers and probes for nucleic acid base detection method of foodborne pathogen
Authors: Soliman Mohamed A. , Azab Mohamed , Hussein Hala A. , Roushdy Mohamed M. , el-naga Mohamed N. Abu 
Published: April 2022

Abstract
Many foods have foodborne pathogens, and detection of it, is very important to serve safe supply of food, consequently reduce of foodborne diseases. Nucleic acid base detection method is one of the most rapid widely methods used in the detection of foodborne pathogens, it depends on the hybridizing between target sequence of the nucleic acid and oligonucleotide primers or probes complementary to the specific nucleic acid sequence of the targeted bacteria. Designing primers and probes for this method is a preliminary and critical step‏.‏ however, new bioinformatics tools are needed to automate, specific and improve the design sets to be used in Nucleic Acid base method. Thus, we developed Foodborne Pathogen primer prob design (FBPP), ‎an open-source Python-based application supported with SQL database for foodborne pathogen virulence factor, for (i) ‎ design primer /probe for detection purpose (ii) PCR & Gel electrophoreses Photo simulation (iii) check the specificity of primers / probe.
Model chain from microscale meteorological simulation to building simulation for spatially resolved heat resilience evaluation
Authors: Schünemann Christoph , Ziemann Astrid , Goldberg Valeri , Kunze Stefanie 
Published: April 2022

Abstract
To assess the heat resilience of buildings in urban development we designed a model chain approach from microscale meteorological simulations, resolving the spatial influences on urban climate, to building performance simulations, evaluating the indoor overheating risk in a building. In this context, the question arises how realistic the microscale meteorological simulation input is and how it affects the indoor comfort. Therefore, we applied the 3D urban climate model ENVI-met and the 1D boundary layer model HIRVAC for two urban districts in Germany as meteorological input for the indoor comfort evaluation of two representative buildings. The results demonstrate that ENVI-met simulations without using measured temperature data create unrealistically low diurnal variations in outdoor air temperature despite an overestimated solar irradiance. The implementation to building simulation leads to a tremendous overestimation of the indoor overheating intensity in both buildings and to wrong conclusions about the efficacy of passive heat adaptation measures. In contrast, the HIRVAC simulations show a more realistic representation of the selected meteorological boundary conditions. Our findings demonstrate that an adjusted boundary layer representation in microscale meteorological simulations is crucial to provide meteorological input suitable for indoor overheating analysis.
Evaluation of the margins and gingival status of full-crown restorations using a household intraoral camera
Authors: Chiu Shuting , Lee Yeh , Liu Min , Chen Hu , Liu Yunsong 
Published: April 2022

Abstract
Objective: The purpose of this study was to compare the usefulness of intraoral photographs, acquired with a household intraoral camera operating in conventional, calibrated, and polarized modes, with clinical examinations for assessing the margins and gingival status of full-crown restorations.Methods: The clinical examination is performed by a prosthodontist expert who classifies the marginal status of full-crown restorations according to the FDI World Dental Federation, and periodontal expert who classifies the gingival status according to the Modified Gingival Index (MGI). The margins and gingival status of the conventional, calibration, and polarization groups of full-crown restorations were independently assessed by three evaluators who obtained photographs using an intraoral camera. Cases where at least two of three assessors were in agreement were analyzed using Cohen’s kappa coefficient and the chi-square test were calculated.Results: This study included 40 patients with 54 full-crown restorations. The conventional, calibration, and polarization groups differed significantly in marginal and gingival status of full-crown restorations. In the calibration group, there was good agreement between the camera-based and oral clinical examinations in terms of the gingival status of full-crown restorations (kappa = 0.945); this was also the case in the polarization group with respect to the margins of full-crown restorations (kappa = 0.917).Conclusions: An intraoral camera with black and white calibrated images is useful to assess the gingival status of full-crown restorations. Polarization mode can be used to assess the margins of full-crown restorations. The camera is a feasible and valid diagnostic aid.
A Novel Method for Prediction of Skin Diseases Using Supervised Classification Techniques
Authors: K Meena , Veni N N Krishna , Deepapriya B S , Vardhini P A Harsha , Kalyani BJD , L Sharmila 
Published: April 2022

Abstract
Skin diseases are the most important worrying problems in societies because it affects the patients both physically and psychologically. Skin disease is one of the highly prone to risk with an association of climatic factors around the world. Predicting the skin disease cases associated with influencing factors is the most crucial task. It is very difficult task to identify the appropriate and optimal features for skin disease from the large volume of health sector data available in the world. Previous researchers applied different types of ensemble features selection techniques for the appropriate selection of features which gives highest accuracy with minimum computation time. Classification rate of any algorithm depends on feature extraction techniques and classifier used for classification purpose. Data availability is one of the most significant drawbacks in the health sector if data is available that might be in raw format. Filling missing value and type conversion almost takes 70% of the time. The missing value can be addressed by statistical parameters such as mean, average, and median with stand mechanism in machine learning. The objective of this paper is the selection of significant attributes and removes irrelevant features that affect model performance. The performance of skin disease data can be experimented through K Nearest Neighbor (KNN), Support Vector Machine (SVM) and random forest classifier. The efficiency of the proposed approach is measured through confusion matrix, accuracy, F-measure, precision and Recall.
Theory and experiment on terahertz vibrational spectrum of ferulic acid
Authors: Chang Tianying , Liang Jie , Wei Dongshan , Cui Hong-Liang 
Published: April 2022

Abstract
Ferulic acid (FA, C10H10O4), a type of hydroxycinnamic acid derivatives, has beneficial pharmacological effects and biological activities, and known for its high application value in medicine, health care and cosmetic products. The vibration spectrum of FA ranging from 0.3 THz to 2.0 THz has been investigated by terahertz time-domain spectroscopy (THz-TDS). The characteristic absorption peaks located at 0.937, 1.159, 1.464, 1.694 and 1.910 THz are obtained experimentally. To understand the origin of the characteristic absorption peaks of FA, density functional theory (DFT) calculations based on both isolated molecule and crystalline structure are performed. Combined analysis of the experimental and theoretical results informs that the characteristic absorption peak at 1.464 THz comes from intra-molecular interaction, and the ones at 0.937, 1.159, 1.694, and 1.910 THz originate from inter-molecular collective vibrational modes.