bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mode-locked ytterbium-doped fiber laser with zinc phthalocyanine thin film saturable absorber
Authors: Soboh Rawan S. M. , Al-Masoodi Ahmed H. H. , Erman Fuad N. A. , Al-Masoodi Abtisam H. H. , Nizamani Bilal , Arof Hamzah , Apsari Retna , Wadi Harun Sulaiman 
Published: June 2022

AbstractA stable mode-locked laser was demonstrated using a newly developed zinc phthalocyanine (ZnPc) thin film as passive saturable absorber (SA) in ytterbium-doped fiber laser (YDFL). The ZnPc thin film was obtained using a casting method and then inserted between the two fiber ferrules of a YDFL ring cavity to generate mode-locked pulses. The resulting pulsed laser operated at a wavelength of 1034.5 nm having a repetition rate of 3.3 MHz. At pump power of 277 mW, the maximum output power and pulse energy are achieved at 4.92 mW and 1.36 nJ, respectively. ZnPc has a high chemical and photochemical stability, and its significance for use as a potential SA in a mode-locked laser is reported in this work.
Graphical Abstract
Combining metabolic phenotype determination with metabolomics and transcriptional analyses to reveal pathways regulated by hydroxycarboxylic acid receptor 2
Authors: Rabe Philipp , Gehmlich Mareike , Peters Anna , Krumbholz Petra , Nordström Anders , Stäubert Claudia 
Published: June 2022

Abstract
Background
The adaptation of cellular metabolism is considered a hallmark of cancer. Oncogenic signaling pathways support tumorigenesis and cancer progression through the induction of certain metabolic phenotypes associated with altered regulation of key metabolic enzymes. Hydroxycarboxylic acid receptor 2 (HCA2) is a G protein-coupled receptor previously shown to act as a tumor suppressor. Here, we aimed to unveil the connection between cellular metabolism and HCA2 in BT-474 cells. Moreover, we intend to clarify how well this metabolic phenotype is reflected in transcriptional changes and metabolite levels as determined by global metabolomics analyses.

Methods
We performed both, siRNA mediated knockdown of HCA2 and stimulation with the HCA2-specific agonist monomethyl fumarate. Seahorse technology was used to determine the role of HCA2 in BT-474 breast cancer cell metabolism and its potential to induce a switch in the metabolic phenotype in the presence of different energy substrates. Changes in the mRNA expression of metabolic enzymes were detected with real-time quantitative PCR (RT-qPCR). Untargeted liquid chromatography-mass spectrometry (LC-MS) metabolic profiling was used to determine changes in metabolite levels.

Results
Knockdown or stimulation of HCA2 induced changes in the metabolic phenotype of BT474 cells dependent on the availability of energy substrates. The presence of HCA2 was associated with increased glycolytic flux with no fatty acids available. This was reflected in the increased mRNA expression of the glycolytic enzymes PFKFB4 and PKM2, which are known to promote the Warburg effect and have been described as prognostic markers in different types of cancer. With exogenous palmitate present, HCA2 caused elevated fatty acid oxidation and likely lipolysis. The increase in lipolysis was also detectable at the transcriptional level of ATGL and the metabolite levels of palmitic and stearic acid.

Conclusions
We combined metabolic phenotype determination with metabolomics and transcriptional analyses and identified HCA2 as a regulator of glycolytic flux and fatty acid metabolism in BT-474 breast cancer cells. Thus, HCA2, for which agonists are already widely used to treat diseases such as psoriasis or hyperlipidemia, may prove useful as a target in combination cancer therapy.
Suicide risk in caregivers of people with dementia: a systematic review and meta-analysis
Authors: Solimando Luisa , Fasulo Marta , Cavallero Stefano , Veronese Nicola , Smith Lee , Vernuccio Laura , Bolzetta Francesco , Dominguez Ligia J. , Barbagallo Mario 
Published: June 2022

AbstractBackgroundInterest in physical and mental health outcomes in caregivers of patients with dementia is increasing. However, there is limited data available on the prevalence of suicidal ideation, suicidal attempts, and suicide rates among caregivers of those with dementia. Therefore, we aimed to systematically review these outcomes to address this gap in the literature and thus provide information to inform future policy and intervention for the benefit of caregivers of dementia patients.MethodsWe searched several databases from inception to the 10thNovember 2021, for studies investigating suicidal ideation, suicidal attempts, and suicide rates of caregivers of patients with dementia. We report data regarding suicidal ideation as prevalence, with the 95% confidence intervals (CIs), applying a random-effect model; since less than three studies were available for suicide attempt and suicide, these data are reported descriptively.ResultsAmong 194 articles, eight comprising 1,209 informal caregivers of people with dementia (mean age: 63.9 years, 74% females) were included. The prevalence of suicide ideation was 32.32% (95% CI: 16.01–48.64%;I2 = 98.5%,p < 0.0001). The prevalence of suicide ideation varied between studies from 4.69% to 77.78%. Two studies reported the rate of suicidal attempt in caregivers of patients with dementia, with the prevalence ranging from 5.9% to 16.1%. One study reported one in 17 caregivers of patients with dementia died by suicide.ConclusionsThe prevalence of suicide ideation is high, affecting several caregivers of patients with dementia. These findings suggest intervention and/or policy are urgently needed to address suicidal behavior in this at-risk population.
A general toxicity and biodistribution study of human natural killer cells by single or repeated intravenous dose in severe combined immune deficient mice
Authors: Park Sang-Jin , Yoon Hae-Jin , Gu Eun-Young , Lee Byoung-Seok , Kim Yongman , Jung Jaeseob , Kim Jinmoon , Moon Kyoung-Sik 
Published: June 2022

AbstractNatural killer (NK) cells are a part of the innate immune system and represent the first line of defense against infections and tumors. NK cells can eliminate tumor cells without major histocompatibility restriction and are independent of the expression of tumor-associated antigens. Therefore, they are considered an emerging tool for cancer immunotherapy. However, the general toxicity and biodistribution of NK cells after transplantation remain to be understood. This study was conducted to evaluate the general toxicity and biodistribution of human NK cells after single or repeated intravenous dosing in severely combined immunodeficient (SCID) mice. There were no test item-related toxicological changes in single and repeated administration groups. The no observed adverse effect level of human NK cells was 2 × 107 cells/head for both male and female SCID mice. Results from the biodistribution study showed that human NK cells were mainly distributed in the lungs, and a small number of the cells were detected in the liver, heart, spleen, and kidney of SCID mice, in both the single and repeated dose groups. Additionally, human NK cells were completely eliminated from all organs of the mice in the single dose group on day 7, while the cells persisted in mice in the repeated dose group until day 64. In conclusion, transplantation of human NK cells in SCID mice had no toxic effects. The cells were mainly distributed in the lungs and completely disappeared from the body over time after single or repeated intravenous administration.
Second chances in antecedent retrieval: The processing of reflexives in two types of reconstruction environments
Authors: Chapman Cassandra , Moulton Keir 
Published: June 2022

AbstractA body of psycholinguistic research shows that structural constraints play a large role in guiding anaphora resolution in the early moments of processing (Nicol and Swinney 1989; Harris et al. 2000; Sturt 2003; Kazanina et al. 2007; Xiang et al. 2009; Chow et al. 2014). Omaki (2010) and Omaki et al. (2019) report on an interesting case where reflexives in wh-predicate fronting constructions launch a search that is not structurally guided. We further investigate this phenomenon, by asking whether non-structurally guided retrievals of this sort result in comprehenders ever committing to ungrammatical antecedents, perhaps as a ‘lingering’ interpretation of the sort found with garden path sentences (Christianson et al. 2001; Ferreira et al. 2001; Slattery et al. 2013). In two forced-choice studies, we find evidence that ungrammatical dependencies resulting from a non-structural search are sometimes maintained in offline comprehension, particularly with a more demanding task. We then probe the incremental processing that follows non-structurally guided retrieval, asking if and when the processor initiates a renewed search. In a self-paced reading experiment, we show that the processor continues its search for an antecedent very soon after retrieving a non-structurally guided antecedent. Surprisingly, however, we found a similar processing profile in cases where a structurally licensed antecedent was already encountered. While it has been recently shown that cataphoric pronouns persist in an antecedent search after a failed retrieval (Giskes and Kush 2021), our results suggest that when reflexives locate a preceding antecedent – by either a structural or non-structural search – this does not terminate further consideration of a different dependency. We consider these data in light of the comparison between cataphoric elements and wh-fillers in launching an active search to complete a dependency.
Towards a Science of Scaling for Urban Climate Action and Governance
Authors: van der Heijden Jeroen 
Published: June 2022

AbstractThe scaling of urban climate action and its governance is rapidly becoming a central focus in the urban climate governance literature and policy debates. Building on the broader scaling literature and inspired by related initiatives in other fields, this article calls for the development of a systematic “science of scaling” for urban climate governance. Such a science of scaling may help to give a better understanding of how well-performing urban climate action and its governance can be multiplied, accelerated and broadened (ie horizontal and vertical scaling and scaling out, up and down), and it may help to uncover scaling trajectories towards systemic change in cities (ie deep scaling).
Surface area matters: An evaluation of swabs and surface area for environmental surface sampling of healthcare pathogens
Authors: West Rolieria M. , Shams Alicia M. , Chan Monica Y. , Rose Laura J. , Noble-Wang Judith A. 
Published: June 2022

AbstractFlocked and foam swabs were used to sample five healthcare pathogens from three sizes of steel and plastic coupons; 26 cm2, 323 cm2, and 645 cm2. As surface area increased, 1–2 log10 decrease in recovered organisms (P < .05) was observed. Sampling 26-cm2 yielded the optimal median percent of pathogens recovered.
Healthy lifestyle changes favourably affect common carotid intima-media thickness: the Healthy Lifestyle Community Programme (cohort 2)
Authors: Koeder Christian , Husain Sarah , Kranz Ragna-Marie , Anand Corinna , Alzughayyar Dima , Schoch Nora , Hahn Andreas , Englert Heike 
Published: June 2022

Abstract
Common carotid intima-media thickness (ccIMT) progression is a risk marker for cardiovascular disease (CVD), whereas healthy lifestyle habits are associated with lower ccIMT. The objective of the present study was to test whether a healthy lifestyle intervention can beneficially affect ccIMT progression. A community-based non-randomised, controlled lifestyle intervention was conducted, focusing on a predominantly plant-based diet (strongest emphasis), physical activity, stress management and social health. Assessments of ccIMT were made at baseline, 6 months and 1 year. Participants had an average age of 57 years and were recruited from the general population in rural northwest Germany (intervention: n 114; control: n 87). From baseline to 1 year, mean ccIMT significantly increased in both the intervention (0⋅026 [95 % CI 0⋅012, 0⋅039] mm) and control group (0⋅045 [95 % CI 0⋅033, 0⋅056] mm). The 1-year trajectory of mean ccIMT was lower in the intervention group (P = 0⋅022; adjusted for baseline). In a subgroup analysis with participants with high baseline mean ccIMT (≥0⋅800 mm), mean ccIMT non-significantly decreased in the intervention group (−0⋅016 [95 % CI −0⋅050, 0⋅017] mm; n 18) and significantly increased in the control group (0⋅065 [95 % CI 0⋅033, 0⋅096] mm; n 12). In the subgroup, the 1-year trajectory of mean ccIMT was significantly lower in the intervention group (between-group difference: −0⋅051 [95 % CI −0⋅075, −0⋅027] mm; P < 0⋅001; adjusted for baseline). The results indicate that healthy lifestyle changes may beneficially affect ccIMT within 1 year, particularly if baseline ccIMT is high.
Comparing 2 crystal structures and 12 AlphaFold2-predicted human membrane glucose transporters and their water-soluble glutamine, threonine and tyrosine variants
Authors: Smorodina Eva , Tao Fei , Qing Rui , Jin David , Yang Steve , Zhang Shuguang 
Published: June 2022

Abstract
Membrane transporters including glucose transporters (GLUTs) are involved in cellular energy supplies, cell metabolism and other vital biological activities. They have also been implicated in cancer proliferation and metastasis, thus they represent an important target in combatting cancer. However, membrane transporters are very difficult to study due to their multispan transmembrane properties. The new computational tool, AlphaFold2, offers highly accurate predictions of three-dimensional protein structures. The glutamine, threonine and tyrosine (QTY) code provides a systematic method of rendering hydrophobic sequences into hydrophilic ones. Here, we present computational studies of native integral membrane GLUTs with 12 transmembrane helical segments determined by X-ray crystallography and CryoEM, comparing the AlphaFold2-predicted native structure to their water-soluble QTY variants predicted by AlphaFold2. In the native structures of the transmembrane helices, there are hydrophobic amino acids leucine (L), isoleucine (I), valine (V) and phenylalanine (F). Applying the QTY code, these hydrophobic amino acids are systematically replaced by hydrophilic amino acids, glutamine (Q), threonine (T) and tyrosine (Y) rendering them water-soluble. We present the superposed structures of native GLUTs and their water-soluble QTY variants. The superposed structures show remarkable similar residue mean square distance values between 0.47 and 3.6 Å (most about 1–2 Å) despite >44% transmembrane amino acid differences. We also show the differences of hydrophobicity patches between the native membrane transporters and their QTY variants. We explain the rationale why the membrane protein QTY variants become water-soluble. Our study provides insight into the differences between the hydrophobic helices and hydrophilic helices, and offers confirmation of the QTY method for studying multispan transmembrane proteins and other aggregated proteins through their water-soluble variants.
Taxonomy and palaeoenvironmental distribution of palaeopascichnids
Authors: Kolesnikov Anton , Desiatkin Vladislav 
Published: June 2022

AbstractPalaeopascichnida is a problematic group of extinct organisms that is globally distributed in Ediacaran sequences of Avalonia, Baltica, Siberia, South China and Australia. The fossils related to Palaeopascichnida consist of serially or cluster-like arranged, millimetre- to centimetre-scale globular or allantoid chambers, which are characterized by substantial differences in preservation, leading to no consistent diagnosis for these organisms. Here we integrate morphometric variation, stratigraphic distribution and habitat settings of more than 1200 specimens from all known fossil localities. The results of the morphological analysis demonstrate variation in chamber shape and size, and allow us to recognize six valid species within the group. Statistical analysis of the specimen distribution with respect to sedimentary environments indicates a significant difference in palaeoecological settings between species, making a significant contribution to the evolution and systematic palaeontology of these problematic organisms and perspective on their use in Neoproterozoic biostratigraphy. Our revision and systematic study sheds new light on one of the least studied groups of the late Ediacaran biota.
Financialisation and private equity in early childhood care and education in England
Authors: SIMON ANTONIA , SHAH ATUL , QUY KATIE , OWEN CHARLIE 
Published: June 2022

AbstractThe Government in England contributes an estimated £3.9 billion funding to support childcare and education for three- and four-year olds and for some two-year olds. A significant proportion of this money is spent on private sector childcare. However, little is known about how the money paid to companies providing private sector childcare is used. Through a cross-case analysis, the financial accounts of a sample of medium-to large private ‘for-profit’ childcare groups were compared with some ‘not-for-profit’ childcare providers. We found that for the for-profit companies, a considerable amount of money is being extracted for debt repayment and relatively little goes into staff wages. We found that large private for-profit nursery groups predominately use ‘private equity’ models which are characterised by borrowings and debt, with a focus on short-term financial returns. This ‘for-profit’ financial operating model arguably risks the sustainability of provision in the sector. Reformed regulation and transparency in the accounting of such providers and a consideration of alternative ‘not-for-profit’ financial models could provide greater stability and resilience.
Access for Augustus: The ‘House of Livia’ and the Palatine Passages
Authors: Wiseman T. P. 
Published: June 2022

AbstractThis article draws attention to a neglected archaeological datum (pointed out by Amanda Claridge in 2014) that has important consequences for our understanding of the Augustan Palatine. The ‘house of Livia’, excavated in 1869, has always been thought of as belonging to the Augustan complex, but the evidence suggests that it did not exist above ground level after the 30sb.c.; its basement, on the other hand, was connected with an underground passage that was evidently an integral part of the building project begun by Imperator Caesar (‘Octavian’) in 36b.c. Claridge's argument, with its corollary, that the Apollo temple faced north-east to the summit plateau of the hill, explains the surviving textual sources much more satisfactorily than the current orthodoxy. In particular, it casts significant new light on Suetonius’ statement that the name ‘Augustus’ referred to Romulus’augustum auguriumat the foundation of the city.
An online scalarization multi-objective reinforcement learning algorithm: TOPSIS Q-learning
Authors: Mirzanejad Mohammad , Ebrahimi Morteza , Vamplew Peter , Veisi Hadi 
Published: June 2022

Abstract
Conventional reinforcement learning focuses on problems with single objective. However, many problems have multiple objectives or criteria that may be independent, related, or contradictory. In such cases, multi-objective reinforcement learning is used to propose a compromise among the solutions to balance the objectives. TOPSIS is a multi-criteria decision method that selects the alternative with minimum distance from the positive ideal solution and the maximum distance from the negative ideal solution, so it can be used effectively in the decision-making process to select the next action. In this research a single-policy algorithm called TOPSIS Q-Learning is provided with focus on its performance in online mode. Unlike all single-policy methods, in the first version of the algorithm, there is no need for the user to specify the weights of the objectives. The user’s preferences may not be completely definite, so all weight preferences are combined together as decision criteria and a solution is generated by considering all these preferences at once and user can model the uncertainty and weight changes of objectives around their specified preferences of objectives. If the user only wants to apply the algorithm for a specific set of weights the second version of the algorithm efficiently accomplishes that.
Rapid star identification algorithm for fish-eye camera based on PPP/INS assistance
Authors: Li Chonghui , Yang Yuanxi , Xiao Guorui , Chen Zhanglei , Tong Shuai , Liu Zihao 
Published: June 2022

AbstractThe fish-eye star sensor with a field of view (FOV) of 180° is an important piece of equipment for attitude determination, which improves the visibility of stars significantly. However, it also brings the star identification (star-ID) difficulties because of imprecise calibrations. Thus, a fish-eye star-ID algorithm supported by the integration of the precise point positioning/inertial navigation system (PPP/INS) is proposed. At first, a reference star map is generated in combination with the distortion model of the fish-eye camera based on the position and attitude information from the PPP/INS. Then the star points are extracted in a specific neighbourhood of the reference star points. Subsequently, the extracted star points are individually tested and identified according to angular distance error. Finally, the real-time precise attitude is determined based on the star-ID results. Experimental results show that, 270–310 stars can be identified in a fish-eye star map with an average time of 0.03 s if the initial attitude error is smaller than 1.5° and an attitude determination accuracy better than 10″ can be achieved by support from PPP/INS.
The popularity of the light comedy during the late era of Kim Jong-il
Authors: Hong Jae-Beom , Paik Seung-Moo , Cho Seong-Kwan 
Published: June 2022

AbstractThis paper examines the political and cultural context of the popular 2010 revival of the light comedy theatre productionSanullimin North Korea. The play, originally written and performed in 1961, portrays the spirit of revolutionary optimism as the characters resolve an unhostile conflict and unite to expand socialist production to contribute to overcoming the (real-life) political and economic crisis of the Chollima era. The 2010 revival of this propaganda responded to similar political and economic crisis, and was designed to instil confidence that the present crisis would be overcome as successfully as in the first Chollima era, provided that people could conjure the same revolutionary optimism. This paper examines why this particular play was revived over others from the Kim Il-sung era, and its particular potential to serve as effective propaganda during the transition from military-first to party-first policy in the Kim Jong-un era, in reference to parallels between 1961 and 2010. The play immerses the audience in the dramatic situations through verisimilitude to the lives of the audience, though the emotional excess of the characters is often exaggerated. Such laughter ignited by dramatic irony contributed to creating a heightened ideological thought of the audience who would spontaneously (re-)internalise the communist human character. The revival of the play was the most appropriate choice according to the object of justification of the succession of power from Kim Jung-il to Kim Jung-un.