bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nipah virus detection at bat roosts following spillover events in Bangladesh, 2012–2019
Authors: McKee Clifton D. , Islam Ausraful , Rahman Mohammed Ziaur , Khan Salah Uddin , Rahman Mahmudur , Satter Syed M. , Islam Ariful , Yinda Claude Kwe , Epstein Jonathan H. , Daszak Peter , Munster Vincent J. , Hudson Peter J. , Plowright Raina K. , Luby Stephen P. , Gurley Emily S. 
Published: December 2021

AbstractKnowledge of the dynamics and genetic diversity of Nipah virus circulating in bats and at the human-animal interface is limited by current sampling efforts, which produce few detections of viral RNA. We report on a series of investigations at bat roosts identified near human Nipah cases in Bangladesh between 2012 and 2019. Pooled bat urine samples were collected from 23 roosts; seven roosts (30%) had at least one sample with Nipah RNA detected from the first visit. In subsequent visits to these seven roosts, RNA was detected in bat urine up to 52 days after the presumed exposure of the human case, although the probability of detection declined rapidly with time. These results suggest that rapidly deployed investigations of Nipah virus shedding from bat roosts near human cases could increase the success of viral sequencing compared to background surveillance and enhance our understanding of Nipah virus ecology and evolution.
Longitudinal analysis of invariant natural killer T cell activation reveals a cMAF-associated transcriptional state of NKT10 cells
Authors: Kane Harry , LaMarche Nelson M. , Scannail Áine Ní , Brenner Michael B. , Lynch Lydia 
Published: December 2021

AbstractInnate T cells, including CD1d-restricted invariant natural killer T (iNKT) cells, are characterized by their rapid activation in response to non-peptide antigens, such as lipids. While the transcriptional profiles of naive, effector and memory adaptive T cells have been well studied, less is known about transcriptional regulation of different iNKT cell activation states. Here, using single cell RNA-sequencing, we performed longitudinal profiling of activated iNKT cells, generating a transcriptomic atlas of iNKT cell activation states. We found that transcriptional signatures of activation are highly conserved among heterogeneous iNKT cell populations, including NKT1, NKT2 and NKT17 subsets, and human iNKT cells. Strikingly, we found that regulatory iNKT cells, such as adipose iNKT cells, undergo blunted activation, and display constitutive enrichment of memory-like cMAF+ and KLRG1+ populations. Moreover, we identify a conserved cMAF-associated transcriptional network among NKT10 cells, providing novel insights into the biology of regulatory and antigen experienced iNKT cells.
First high-quality genome assembly data of sago palm (Metroxylon sagu Rottboll)
Authors: Kit Lim Leonard Whye , Lin Lau Melinda Mei , Chung Hung Hui , Hussain Hasnain , Gan Han Ming 
Published: December 2021

AbstractThe sago palm (Metroxylon sagu Rottboll) is a all-rounder palm, it is both a tropical halophytic starch-producing palm as well as an ornamental plant. Recently, a genome survey was conducted on this palm using Illumina sequencing platform but the BUSCO genome completeness is very low (21.5%) and most of them (∼78%) are either fragmented or missing. Thus, in this study, the sago palm genome completeness was further improved with the utilization of the Nanopore sequencing platform that produced longer reads. A hybrid genome assembly was conducted and the outcome was a much complete sago palm genome with BUSCO completeness achieved at as high as 97.9% with only ∼2% of them either fragmented or missing. The estimated genome size of the sago palm is 509,812,790 bp in this study. A sum of 33,242 protein-coding genes were revealed from the sago palm genome and around 96.39% of them had been functionally annotated. An investigation on the carbohydrate metabolism KEGG pathways also unearthed that starch synthesis was one of the major sago palm activities. These data are indispensable for future molecular evolutionary and genome-wide association studies.Specifications TableValue of the DataFirst complete genome dataset for the eco-economic important sago palm (Metroxylon sagu Rottboll).High completeness of sago palm genomic dataset will facilitate future researches, such as genome-wide association studies.The data is useful in pioneering sago palm genetic landscape investigations which in turn unmask the mystery behind its high starch yield, salinity tolerance and disease resistance.
Depletion of CD206+Tumour Macrophages via a Peptide-Targeted Star-Shaped Polyglutamate Inhibits Tumourigenesis and Metastatic Dissemination in Breast Cancer Models
Authors: Lepland Anni , Malfanti Alessio , Haljasorg Uku , Asciutto Eliana K. , Pickholz Monica , Bringas Mauro , Đorđević Snežana , Salumäe Liis , Peterson Pärt , Teesalu Tambet , Vicent María J. , Scodeller Pablo 
Published: December 2021

ABSTRACTAlthough many studies have explored the depletion of tumour-associated macrophages (TAMs) as a therapeutic strategy for solid tumours, currently available compounds suffer from poor efficacy and dose-limiting side effects. Here, we developed a novel TAM-depleting agent (“OximUNO”) that specifically targets CD206+TAMs and demonstrated efficacy in triple negative breast cancer (TNBC) mouse models. OximUNO comprises a star-shaped polyglutamate (St-PGA) decorated with the CD206-targeting peptide mUNO that carries the chemotherapeutic drug doxorubicin (DOX). In TNBC models, a fluorescently labelled mUNO-decorated St-PGA homed to CD206+TAMs within primary lesions and metastases. OximUNO exhibited no acute liver or kidney toxicity in vivo. Treatment with OximUNO reduced the progression of primary tumour lesions and pulmonary metastases, significantly diminished the number of CD206+TAMs and increased the CD8/FOXP3 expression ratio (demonstrating immunostimulation). Our findings suggest the potential benefit of OximUNO as a TAM-depleting agent for TNBC treatment. Importantly, our studies also represent the first report of a peptide-targeted St-PGA as a targeted therapeutic nanoconjugate.
The evolution and biological correlates of hand preferences in anthropoid primates
Authors: Caspar Kai R. , Pallasdies Fabian , Mader Larissa , Sartorelli Heitor , Begall Sabine 
Published: December 2021

AbstractThe evolution of human right-handedness has been intensively debated for decades. Manual lateralization patterns in non-human primates have the potential to elucidate evolutionary determinants of human handedness. However, restricted species samples and inconsistent methodologies have so far limited comparative phylogenetic studies. By combining original data with published literature reports, we assembled data on hand preferences for standardized object manipulation in 1,786 individuals from 38 species of anthropoid primates, including monkeys, apes, and humans. Based on that, we employ quantitative phylogenetic methods to test prevalent hypotheses on the roles of ecology, brain size and tool use in primate handedness evolution. We confirm that human right-handedness represents an unparalleled extreme among anthropoids and found taxa displaying population-level handedness to be rare. Species-level direction of manual lateralization was largely uniform among non-human primates and did not strongly correlate with any of the selected biological predictors, nor with phylogeny. In contrast, we recovered highly variable patterns of hand preference strength, which show signatures of both ecology and phylogeny. In particular, terrestrial primates tend to display weaker hand preferences than arboreal species. These results challenge popular ideas on primate handedness evolution, especially the postural origins hypothesis. Furthermore, they point to a potential adaptive benefit of disparate lateralization strength in primates, a measure of hand preference that has often been overlooked in the past. Finally, our data show that human lateralization patterns do not align with trends found among other anthropoids, suggesting that unique selective pressures gave rise to the unusual hand preferences of our species.
Impact of circadian time of dosing on cardiomyocyte-autonomous effects of glucocorticoids
Authors: Wintzinger Michelle , Panta Manoj , Miz Karen , Pragasam Ashok D. , Durumutla Hima , Sargent Michelle , Peek Clara Bien , Bass Joseph , Molkentin Jeffery D. , Quattrocelli Mattia 
Published: December 2021

AbstractMitochondrial capacity is critical to adapt the high energy demand of the heart to circadian oscillations and diseased states. Glucocorticoids regulate the circadian cycle of energy metabolism, but little is known about how circadian timing of exogenous glucocorticoid dosing directly regulates heart metabolism through cardiomyocyte-autonomous mechanisms. While chronic oncedaily intake of glucocorticoids promotes metabolic stress and heart failure, we recently discovered that intermittent once-weekly dosing of exogenous glucocorticoids promoted muscle metabolism in normal and obese skeletal muscle. However, the effects of glucocorticoid intermittence on heart metabolism and heart failure remain unknown. Here we investigated the extent to which circadian time of dosing regulates the effects of the glucocorticoid prednisone in heart metabolism and function in conditions of single pulse or chronic intermittent dosing. In WT mice, we found that prednisone improved cardiac content of NAD+and ATP with light-phase dosing (ZT0), while the effects were blocked by dark-phase dosing (ZT12). The drug effects on mitochondrial function were cardiomyocyte-autonomous, as shown by inducible cardiomyocyte-restricted glucocorticoid receptor (GR) ablation, and depended on an intact cardiomyocyte clock, as shown by inducible cardiomyocyte-restricted ablation of Brain and Muscle ARNT-like 1 (BMAL1). Conjugating time-of-dosing with chronic intermittence, we found that once-weekly prednisone improved metabolism and function in heart after myocardial injury dependent on circadian time of intake, i.e. with lightphase but not dark-phase dosing. Our study identifies cardiac-autonomous mechanisms through which circadian-specific intermittent dosing reconverts glucocorticoid drugs to metabolic boosters for the heart.
Comparative transcriptomics reveals the molecular toolkit used by an algivorous protist for cell wall perforation
Authors: Gerbracht Jennifer V. , Harding Tommy , Simpson Alastair G. B. , Roger Andrew J. , Hess Sebastian 
Published: December 2021

SummaryMicrobial eukaryotes display a stunning diversity of feeding strategies, ranging from generalist predators to highly specialised parasites. The unicellular “protoplast feeders” represent a fascinating mechanistic intermediate, as they penetrate other eukaryotic cells (algae, fungi) like some parasites, but then devour their cell contents by phagocytosis. Besides prey recognition and attachment, this complex behaviour involves the local, pre-phagocytotic dissolution of the prey cell wall, which results in well-defined perforations of species-specific size and structure. Yet, the molecular processes that enable protoplast feeders to overcome cell walls of diverse biochemical composition remain unknown. We used the flagellate Orciraptor agilis (Viridiraptoridae, Rhizaria) as a model protoplast feeder, and applied differential gene expression analysis to examine its penetration of green algal cell walls. Besides distinct expression changes that reflect major cellular processes (e.g. locomotion, cell division), we found lytic carbohydrate-active enzymes that are highly expressed and upregulated during the attack on the alga. A putative endocellulase (family GH5_5) with a secretion signal is most prominent, and a potential key factor for cell wall dissolution. Other candidate enzymes (e.g. lytic polysaccharide monooxygenases) belong to families that are largely uncharacterised, emphasising the potential of non-fungal micro-eukaryotes for enzyme exploration. Unexpectedly, we discovered various chitin-related factors that point to an unknown chitin metabolism in Orciraptor, potentially also involved in the feeding process. Our findings provide first molecular insights into an important microbial feeding behaviour, and new directions for cell biology research on non-model eukaryotes.
Adhesion shear strength test method for freshwater/seawater ice on carbon ceramic brake pads of amphibious aircraft
Authors: Zhang Y.J. , Guo Renzhong , Zhang Yunhui , Liang K. 
Published: December 2021

Purpose
Based on the mechanical model of typical shear tests, this study aims to propose the test principle and method of freshwater/seawater ice adhesion shear strength of carbon ceramic brake pads for amphibious aircraft, designs and builds the test equipment, prepares the freshwater/seawater ice samples and completes the tests.

Design/methodology/approach
This study examines the influence of the icing process, mechanism, temperature and freshwater/seawater on ice adhesion shear strength of carbon ceramic brake pads and puts forward a test method for the freshwater/seawater ice adhesion shear strength of amphibious aircraft brake pads.

Findings
The obtained results examine the influence of the icing process, mechanism, temperature and freshwater/seawater on ice adhesion shear strength of carbon ceramic brake pads. The adhesion shear strength of frozen freshwater and of the seawater of Dalian, Qingdao, Fuzhou and Zhuhai on the surface of aircraft brake pads is measured at –10 to –50°C. It is found that the shear strength of freshwater increases first and then decreases with the decrease of temperature. The adhesion shear strength of seawater; however, increases mainly linear with the decrease of temperature.

Originality/value
The value of this paper is that the test method proposed and test results for the freshwater/seawater ice adhesion shear strength of amphibious aircraft brake pads provide technical support for the anti-icing design of amphibious aircraft brake devices.
Fashion involvement, opinion-seeking and product variety as stimulators for fashion e-commerce: an investigated model based on S-O-R model
Authors: Alanadoly Alshaimaa , Salem Suha 
Published: December 2021

PurposeThis study's aim was to investigate the stimulators of fashion e-consumers within e-commerce environments. The study proposed a framework utilizing stimulus–organism–response (S-O-R) theory to suggest that fashion involvement and opinion-seeking would act as sociopsychological stimuli, while product variety as an objective stimulus. Perceived quality is proposed as an organism, moderated by perceived price. Consumer buying behavior within e-commerce environments presents the framework's response. The study looked at variables with deeper insights into Malaysian fashion consumers.Design/methodology/approachA quantitative method was used to assess the significance of relationships within the proposed model. Partial least squares structural equation modeling technique was implemented to assess the framework's relationships with a sample size of 374.FindingsResults indicate that fashion involvement is significantly associated as a sociopsychological stimulus, with product variety being an objective stimulus for Malaysian fashion e-consumers. Perceived quality is significantly represented as an organism through the framework, and buying behavior is the latent response. Price would significantly moderate the relationship between fashion involvement and quality. Opinion-seeking was found not to be a significant stimulus for Malaysian e-consumers.Originality/valueContribution of this study goes to the existing literature by providing a deeper understanding of Malaysian e-consumer behavior by applying S-O-R theory. Malaysian fashion e-consumerism was suggested to be influenced by product involvement, quality, price, opinion-seeking and product range offered; therefore, a proposed framework was demonstrated and tested.
Prevalence of malnutrition among women and adolescent girls: impact of urbanization in rural–urban interface of Bangalore
Authors: Bellundagi Veerabhadrappa , Umesh K.B. , Ashwini B.C. , Hamsa K.R. 
Published: December 2021

PurposeIndia is facing a double burden of malnutrition, i.e. undernutrition and obesity. Women and adolescent health and nutrition are very relevant issues which have not received much attention it deserves in India, especially in the context of a girl child. Hence, the purpose of this study is to assess malnutrition among women and adolescent girls as well as the associated factors.Design/methodology/approachAn attempt was made to assess malnutrition among women and adolescent girls and associated factors. The required data was collected from the north (616) and south transects (659) sample households of Bangalore constituting a total sample size of 1,275. The data was analyzed by adopting multiple linear regression and multinomial logistic regression analysis using STATA software.FindingsThe effects of this study simply confirmed that, urbanization had an instantaneous effect on dietary repute of women and adolescent girls, while transferring throughout the gradient from rural to urban with the growing significance of weight problems and obese. In adolescent girls, about 31% were underweight followed by normal weight and overweight across rural–urban interface of Bangalore. The factors such as education, consumption of meat and animal products, a dummy for urban, diabetes and blood pressure were significantly and positively influencing the nutritional status (Body Mass Index) of women across rural–urban interface. While consumption of vegetables, wealth index and per capita income had a positive and significant influence on the nutritional status of adolescent girls.Originality/valueWith limited studies and data available in Karnataka, especially in Bengaluru, one of the fastest growing cities in the world. Against this backdrop, the study was conducted to assess the prevalence of malnutrition among women and adolescents and its association with various socio-economic variables.
Evolution of the rural social security system in a large country over 35 years: institutional transformation and the Chinese experience
Authors: Huo Xuan , Lin Mingang 
Published: December 2021

Purpose This paper aims to review the institutional evolution of China's rural social security system in the past 35 years, focusing on major policy transformations of the rural social security system in a large country, and extract the Chinese experience on developing rural social security.Design/methodology/approach This paper systematically reviews the evolution of rural social security system via organizing policy documents, key events and research literature on the construction of rural social security since the Reform and Opening-up.Findings First, institutional transformation is a profound change in China's rural social security system; second, policy transformation has taken place in the main areas of China's rural social security system, including rural endowment insurance, medical insurance and social assistance; third, the policy evolution of China's rural social security system has a unique experience in a large country.Originality/value China is the country with the largest rural population in the world. The process and experience of transforming the social security system in the vast rural areas of China have important value and reference significance for other developing countries.
Torn between individual aspirations and the family legacy – individual career development in family firms
Authors: Achtenhagen Leona , Haag Kajsa , Hultén Kajsa , Lundgren Jen 
Published: December 2021

PurposeThe purpose of this paper is to explore individual career management by family members in the context of their family firms.Design/methodology/approachThe interpretative interview study of family members active in family businesses explores how this context affects the choice, planning, goals and development of family members' careers in their family business.FindingsThe authors find that career management in the family business setting focuses on fulfilling the family business goals rather than the personal goals of family members. Career management is rather reactive and less self-directed than current literature on career development recommends. Based on the results, the authors develop a process model for individual career management in the family business context.Originality/valueLittle is known about individual career management of family members in a family business context, as research on careers in family firms has so far focused mainly on transgenerational succession. The authors explore how in family firms, the trend towards self-directed, individual career planning is in tension with a commitment to the family business and its legacy.
Investigating the determining factors of sustainable FDI in Vietnam
Authors: Quang Phung Thanh , Rasoulinezhad Ehsan , Linh Nguyen Nhat , Thao Doan Phuong 
Published: December 2021

PurposeThe main purpose of this paper is to analyze the sustainable inward FDI pattern of Vietnam.Design/methodology/approachThis paper intends to analyze the sustainable FDI pattern of Vietnam using the gravity theory and panel data approach for the annual data over the period of 2007–2020.FindingsVietnamese FDI volume is positively affected by political and social factors, globalization and green energy consumption, while geographical distance is a major obstacle to the increase of FDI inflows of the country.Practical implicationsAs the main practical policy implications, issuing policies for sustainable economic growth, launching the novel strategy of green FDI neighborhood policy and regionalism through free trade agreements are recommended.Originality/valueTo the best of author's knowledge, there has not been any in-depth academic study focusing on the Vietnam's sustainable FDI. In addition, three robustness checks have been conducted to ensure the validation of empirical findings.