bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine learning and expression analyses reveal circadian clock features predictive of anxiety
Authors: Zafar Aziz , Overton Rebeccah , Attia Ziad , Ay Ahmet , Ingram Krista 
Published: April 2022

AbstractMood disorders, including generalized anxiety disorder, are associated with disruptions in circadian rhythms and are linked to polymorphisms in circadian clock genes. Molecular mechanisms underlying these connections may be direct—via transcriptional activity of clock genes on downstream mood pathways in the brain, or indirect—via clock gene influences on the phase and amplitude of circadian rhythms which, in turn, modulate physiological processes influencing mood. Employing machine learning combined with statistical approaches, we explored clock genotype combinations that predict risk for anxiety symptoms in a deeply phenotyped population. We identified multiple novel circadian genotypes predictive of anxiety, with thePER3(rs17031614)-AG/CRY1(rs2287161)-CG genotype being the strongest predictor of anxiety risk, particularly in males. Molecular chronotyping, using clock gene expression oscillations, revealed that advanced circadian phase and robust circadian amplitudes are associated with high levels of anxiety symptoms. Further analyses revealed that individuals with advanced phases and pronounced circadian misalignment were at higher risk for severe anxiety symptoms. Our results support both direct and indirect influences of clock gene variants on mood: while sex-specific clock genotype combinations predictive of anxiety symptoms suggest direct effects on mood pathways, the mediation ofPER3effects on anxiety via diurnal preference measures and the association of circadian phase with anxiety symptoms provide evidence for indirect effects of the molecular clockwork on mood. Unraveling the complex molecular mechanisms underlying the links between circadian physiology and mood is essential to identifying the core clock genes to target in future functional studies, thereby advancing the development of non-invasive treatments for anxiety-related disorders.
Whole-genome resequencing of Sorghum bicolor and S. bicolor × S. halepense lines provides new insights for improving plant agroecological characteristics
Authors: Habyarimana Ephrem , Gorthy Sunita , Baloch Faheem S. , Ercisli Sezai , Chung Gyuhwa 
Published: April 2022

AbstractSorghum (Sorghum bicolor L. (Moench)) is the world’s fifth economically most important cereal and is a staple particularly in the semi-arid tropics of Africa and Asia. Genetic gains in this crop can benefit from wild relatives such as Sorghum halepense. Genome sequences including those from this wild species can boost the study of genome-wide and intraspecific variation for dissecting the genetic basis and improving important traits in sorghum. The whole-genome resequencing carried out in this work on a panel of 172 populations of S. bicolor and S. bicolor × S. halepense (SbxSh) advanced lines generated a total of 567,046,841 SNPs, 91,825,474 indels, 1,532,171 SVs, and 4,973,961 CNVs. Clearly, SbxSh accumulated more variants and mutations with powerful effects on genetic differentiation. A total of 5,548 genes private to SbxSh mapped to biological process GO enrichment terms; 34 of these genes mapped to root system development (GO: 0022622). Two of the root specific genes i.e., ROOT PRIMORDIUM DEFECTIVE 1 (RPD1; GeneID: 8054879) and RETARDED ROOT GROWTH (RRG, GeneID: 8072111), were found to exert direct effect on root growth and development. This is the first report on whole-genome resequencing of a sorghum panel that includes S. halepense genome. Mining the private variants and genes of this wild species can provide insights capable of boosting sorghum genetic improvement, particularly the perenniality trait that is compliant with agroecological practices, sustainable agriculture, and climate change resilience.
UV-induced local immunosuppression in the tumour microenvironment of eccrine porocarcinoma and poroma
Authors: Puttonen Maya , Isola Jorma , Ylinen Onni , Böhling Tom , Koljonen Virve , Sihto Harri 
Published: April 2022

AbstractEccrine porocarcinoma (EPC) is a rare malignant adnexal tumour of the skin. Part of EPCs develop from their benign counterpart, poroma (EP), with chronic light exposure and immunosuppression hypothesized to play a role in the malignant transformation. However, the impact of chronic light exposure on the microenvironment of EPCs and EPs has not been investigated yet. Although the clinical relevance of tumour infiltrating lymphocytes (TILs) and tertiary lymphoid structures (TLSs) has been established in various tumours, their distribution and significance in EPCs and EPs is still poorly understood. We characterized the distribution of TILs and TLSs using CD3, CD4, CD8, CD20 immunohistochemistry in a cohort of 10 EPCs and 49 EPs. We then classified our samples using solar-elastosis grading, analyzing the influence of ultraviolet (UV) damage on TIL density. A negative correlation between UV damage and TIL density was observed (CD4 r = −0.286, p = 0.04. CD8 r = −0.305, p = 0.033). No significant difference in TIL density was found between EPCs and EPs. TLS was scarse with the presence rate 10% in EPCs and 8.3% in EPs. The results suggest that UV has an immunosuppressive effect on the microenvironment of EPCs and EPs.
Label free identification of the different status of anemia disease using optimized double-slot cascaded microring resonator
Authors: Bahadoran Mahdi , Seyfari Abbas Kalate , Sanati Parisa , Chua Lee Suan 
Published: April 2022

AbstractAn optical-based label-free biosensors including two indirectly coupled double-slot-waveguide-based microring resonator was designed and optimized for sensing purpose. Then, the optimized system was applied for the detection of hemoglobin concentration in anemia disease. The results were simulated based on the variational finite-difference time domain (varFDTD) method using the Lumerical software (Mode solutions) and the optimum geometrical parameters were determined to realize an optimum light transmission via the sensor. Nine different concentrations of hemoglobin in men and women were applied into the sensor and the status of anemia was identified based on the patients’ gender and different status of anemia disease, including the normal, mild, moderate, severe and life-threatening status. A sensitivity as high as 1024 nm/RIU with the minimum deflection limit of 4.88 × 10–6 RIU were measured for this biosensor, which introduces a high precision and micro-scale lab-on-a-chip micro device for health monitoring of the anemia.
Safety and efficacy of terlipressin in acute-on-chronic liver failure with hepatorenal syndrome-acute kidney injury (HRS-AKI): a prospective cohort study
Authors: Kulkarni Anand V. , Ravikumar Sowmya Tirumalige , Tevethia Harshvardhan , Premkumar Madhumita , Kumar Karan , Sharma Mithun , Gupta Rajesh , Rao Padaki Nagaraja , Reddy Duvvuru Nageshwar 
Published: April 2022

AbstractTerlipressin with albumin, the recommended treatment for hepatorenal syndrome-acute kidney injury (HRS-AKI), is associated with adverse events. Furthermore, the course of AKI in patients with acute-on-chronic liver failure (ACLF) is unknown. We aimed to analyze the safety and efficacy of terlipressin infusion and AKI course in patients with ACLF. We prospectively enrolled consecutive adult patients with ACLF with HRS-AKI (satisfying EASL criteria) treated with terlipressin infusion between 14 October 2019 and 24 July 2020. The objectives were to assess the incidence of adverse events, response to terlipressin, course of HRS-AKI and predictors of mortality. A total of 116 patients were included. Twenty-one percent of patients developed adverse effects. Only 1/3rd of patients who developed adverse events were alive at day 90. Sixty-five percent of the patients responded to terlipressin. Nearly 22% developed recurrence of HRS, and 5.2% progressed to HRS-chronic kidney disease. TFS was 70.4% at day 30 and 57.8% at day 90. On multivariate stepwise Cox regression analysis terlipressin non-response (hazard ratio [HR], 3.49 [1.85–6.57]; P < 0.001) and MELD NA score (HR,1.12 [1.06–1.18]; P < 0.001) predicted mortality at day-90. Patients with ACLF who develop terlipressin related adverse events have dismal prognoses. Terlipressin non-response predicts mortality in patients with ACLF and HRS-AKI.
Nutrient sources differ in the fertilised eggs of two divergent broiler lines selected for meat ultimate pH
Authors: Petit Angélique , Réhault-Godbert Sophie , Nadal-Desbarats Lydie , Cailleau-Audouin Estelle , Chartrin Pascal , Raynaud Emilie , Jimenez Justine , Tesseraud Sophie , Berri Cécile , Le Bihan-Duval Elisabeth , Métayer-Coustard Sonia 
Published: April 2022

AbstractThe pHu+ and pHu− lines, which were selected based on the ultimate pH (pHu) of the breast muscle, represent a unique model to study the genetic and physiological controls of muscle energy store in relation with meat quality in chicken. Indeed, pHu+ and pHu− chicks show differences in protein and energy metabolism soon after hatching, associated with a different ability to use energy sources in the muscle. The present study aimed to assess the extent to which the nutritional environment of the embryo might contribute to the metabolic differences observed between the two lines at hatching. Just before incubation (E0), the egg yolk of pHu+ exhibited a higher lipid percentage compared to the pHu− line (32.9% vs. 27.7%). Although 1H-NMR spectroscopy showed clear changes in egg yolk composition between E0 and E10, there was no line effect. In contrast, 1H-NMR analysis performed on amniotic fluid at embryonic day 10 (E10) clearly discriminated the two lines. The amniotic fluid of pHu+ was richer in leucine, isoleucine, 2-oxoisocaproate, citrate and glucose, while choline and inosine were more abundant in the pHu− line. Our results highlight quantitative and qualitative differences in metabolites and nutrients potentially available to developing embryos, which could contribute to metabolic and developmental differences observed after hatching between the pHu+ and pHu− lines.
A comparison of international and national references to measure the prevalence of stunting in Pakistani school-age girls
Authors: Qaisar Rizwan , Karim Asima 
Published: April 2022

AbstractEpidemiology of stunting in < 5 years old is well characterized; however, its prevalence in adolescence is inconsistent in different geographical locations. We estimated the prevalence of stunting in schoolgirls of Punjab, Pakistan, to standardize local references according to international and national references. In this population-wide cross-sectional study, 10,050 schoolgirls aged 8–16 years from 12 different districts of northern, central, and southern Punjab were analyzed. The prevalence of stunting was calculated by applying Centres for Disease Control and Prevention (CDC) and World Health Organisation (WHO) height-for-age references and the local reference for the study population. We used Cohen’s kappa statistics to analyze the agreement of our data with reference values, and chi-square test was used as the test of trend. Marked overestimation of the prevalence of stunting was observed (22.72% and 17.49% according to CDC and WHO, respectively) in comparison to local reference (4.94%). According to CDC and WHO references, there was an increasing trend of prevalence of stunting with higher age; however, data was comparable across all the age groups when local references were applied. We recommend that the prevalence of stunting in school-age girls should be determined by applying local height references rather than international ones to plan health strategies and treatments in the local population.
Developmental pyrethroid exposure and age influence phenotypes in a Chd8 haploinsufficient autism mouse model
Authors: Jiménez Jessica A. , Simon Jeremy M. , Hu Wenxin , Moy Sheryl S. , Harper Kathryn M. , Liu Chih-Wei , Lu Kun , Zylka Mark J. 
Published: April 2022

AbstractHundreds of genes have been associated with autism spectrum disorder (ASD), including loss-of-function mutations in chromodomain helicase DNA binding protein 8 (Chd8). Environmental factors also are implicated in autism risk and have the potential to exacerbate phenotypes in genetically sensitized backgrounds. Here we investigate transcriptional and behavioral phenotypes in a Chd8 haploinsufficient (Chd8V986*/+) mouse line exposed to the pesticide deltamethrin (DM) from conception to postnatal day 22. Vehicle-exposed Chd8V986*/+ mice displayed ASD-associated phenotypes, including anxiety-like behavior and altered sociability, replicating a previous study with this mouse line. A core set of genes was altered in Chd8V986*/+ mice at multiple ages, including Usp11, Wars2, Crlf2, and Eglf6, and proximity ligation data indicated direct binding of CHD8 to the 5’ region of these genes. Moreover, oligodendrocyte and neurodegenerative transcriptional phenotypes were apparent in 12 and 18 month old Chd8V986*/+ mice. Following DM exposure, the mutant mice displayed an exacerbated phenotype in the elevated plus maze, and genes associated with vascular endothelial cells were downregulated in the cerebral cortex of older Chd8V986*/+ animals. Our study reveals a gene x environment interaction with a Chd8 haploinsufficient mouse line and points to the importance of investigating phenotypes in ASD animal models across the lifespan.
Longitudinal follow-up study of the association between statin use and chronic periodontitis using national health screening cohort of Korean population
Authors: Kwon Mi Jung , Byun Soo-Hwan , Kim Joo-Hee , Kim Ji Hee , Kim Se Hoon , Kim Nan Young , Park Hye-Rim , Choi Hyo Geun 
Published: April 2022

AbstractSince a potential link between statins and the risk of adverse chronic periodontitis (CP) has been raised, we aimed to validate the association between statin use and the incidence of CP using nationwide cohort data. This longitudinal follow-up study included 169,381 patients prescribed statins who were matched with an equal number of controls using propensity scores from the Korean National Health Insurance Service-Health Screening Cohort database (2002–2015). A Cox proportional hazard model was used to assess the occurrence of CP following statin use after adjusting for multiple covariates. The occurrence of CP was significantly higher in patients who had long-term use (1–3 years, 3–5 years, or > 5 years) than with short-term use (≤ 1 year) of statins. After adjustment, statin users exhibited an occurrence of CP 1.32-fold higher (95% confidence interval 1.30–1.33) than that of the matched nonusers (incidence: 25.0 and 22.0 per 100 person-years, respectively). Subgroup analyses supported the adverse impact of statins on CP independent of age and gender. Statin user odds ratios for developing CP were higher compared to those of nonusers. This was consistent in individuals aged > 40 years in both genders, especially with long-term use.
Gold nanoclusters eliminate obesity induced by antipsychotics
Authors: He Meng , Yao Jing , Zhang Zijun , Zhang Ying , Chen Rui , Gu Zhenhua , Huang XuFeng , Deng Chao , Zhou Ruqin , Fan Jun , Zhang Baohua , Xie Yanqian , Gao Guanbin , Sun Taolei 
Published: April 2022

AbstractObesity induced by antipsychotics have plagued more than 20 million people worldwide. However, no drug is available to eliminate the obesity induced by antipsychotics. Here we examined the effect and potential mechanisms of a gold nanoclusters (AuNCs) modified by N-isobutyryl-L-cysteine on the obesity induced by olanzapine, the most prescribed but obesogenic antipsychotics, in a rat model. Our results showed that AuNCs completely prevented and reversed the obesity induced by olanzapine and improved glucose metabolism profile in rats. Further mechanism investigations revealed that AuNCs exert its anti-obesity function through inhibition of olanzapine-induced dysfunction of histamine H1 receptor and proopiomelanocortin signaling therefore reducing hyperphagia, and reversing olanzapine-induced inhibition of uncoupling-protein-1 signaling which increases thermogenesis. Together with AuNCs’ good biocompatibility, these findings not only provide AuNCs as a promising nanodrug candidate for treating obesity induced by antipsychotics, but also open an avenue for the potential application of AuNCs-based nanodrugs in treating general obesity.
Comprehensive genomics in androgen receptor-dependent castration-resistant prostate cancer identifies an adaptation pathway mediated by opioid receptor kappa 1
Authors: Makino Yuki , Kamiyama Yuki , Brown J. B. , Tanaka Toshiya , Murakami Ryusuke , Teramoto Yuki , Goto Takayuki , Akamatsu Shusuke , Terada Naoki , Inoue Takahiro , Kodama Tatsuhiko , Ogawa Osamu , Kobayashi Takashi 
Published: April 2022

AbstractCastration resistance is a lethal form of treatment failure of prostate cancer (PCa) and is associated with ligand-independent activation of the androgen receptor (AR). It is only partially understood how the AR mediates survival and castration-resistant growth of PCa upon androgen deprivation. We investigated integrative genomics using a patient-derived xenograft model recapitulating acquired, AR-dependent castration-resistant PCa (CRPC). Sequencing of chromatin immunoprecipitation using an anti-AR antibody (AR-ChIP seq) revealed distinct profiles of AR binding site (ARBS) in androgen-dependent and castration-resistant xenograft tumors compared with those previously reported based on human PCa cells or tumor tissues. An integrative genetic analysis identified several AR-target genes associated with CRPC progression including OPRK1, which harbors ARBS and was upregulated upon androgen deprivation. Loss of function of OPRK1 retarded the acquisition of castration resistance and inhibited castration-resistant growth of PCa both in vitro and in vivo. Immunohistochemical analysis showed that expression of OPRK1, a G protein-coupled receptor, was upregulated in human prostate cancer tissues after preoperative androgen derivation or CRPC progression. These data suggest that OPRK1 is involved in post-castration survival and cellular adaptation process toward castration-resistant progression of PCa, accelerating the clinical implementation of ORPK1-targeting therapy in the management of this lethal disease.
A flood-crest forecast prototype for river floods using only in-stream measurements
Authors: Muste Marian , Kim Dongsu , Kim Kyungdong 
Published: April 2022

AbstractStreamflow forecasting generally relies on coupled rainfall-runoff-routing models calibrated and executed with data estimated by monitoring protocols that do not fully capture the dynamics of unsteady flows. This limits the ability to accurately forecast flood crests and issue hazard warnings. Here we utilize directly measured datasets acquired for streamflow estimation to develop a data-driven forecasting algorithm that does not require conventional physically-based modeling. We test the potential of our algorithm using measurements acquired at an index-velocity gaging station on the Illinois River, USA, between 2014 and 2019. We find that the forecasting protocol is able to deliver short-term predictions of flood crest magnitude and arrival time. The algorithm produces better agreement with larger events and is more reliable for single-peak storms possibly due to the prominence of hysteretic behavior in such events. We conclude that flood hazard can be forecast using directly measured index-velocity and stage alone.
The personality traits of self-made and inherited millionaires
Authors: Leckelt Marius , König Johannes , Richter David , Back Mitja D. , Schröder Carsten 
Published: April 2022

AbstractVery wealthy people influence political and societal processes by wielding their economic power through foundations, lobbying groups, media campaigns, as investors and employers. Because personality shapes goals, attitudes, and behaviour, it is important to understand the personality traits that characterize the rich. We used representative survey data to construct two large samples, one from the general population and one consisting of individuals with at least 1 million euros in individual net wealth, to analyse what personality traits characterize the wealthy and why their traits differ from those of the general population. High wealth was associated with higher Risk tolerance, Emotional Stability, Openness, Extraversion, and Conscientiousness. This “rich” personality profile was more prominent among individuals who had accumulated wealth through their own efforts (“self-mades”) than among individuals who had been born into wealth (“inheritors”). Thus, our evidence is suggestive of a unique configuration of personality traits contributing to self-made millionaires’ economic success.
Behaviors of sodium and calcium ions at the borosilicate glass–water interface: Gaining new insights through an ab initio molecular dynamics study
Authors: Jabraoui Hicham , Charpentier Thibault , Gin Stéphane , Delaye Jean-Marc , Pollet Rodolphe 
Published: April 2022

We study reactivity and leaching at the calcium sodium borosilicate (CNBS)–water interface by means of a Car–Parrinello ab initio molecular dynamics simulation over a simulation time of 100 ps. With an emphasis on the comparison between the behaviors of Ca2+ and Na+ cations at the CNBS glass–water interface, different mechanism events during the trajectory are revealed, discussed, and correlated with other density functional theory calculations. We show that Na+ ions can be released in solution, while Ca2+ cannot leave the surface of CNBS glass. This release is correlated with the vacancy energy of Ca2+ and Na+ cations. Here, we found that the CNBS structure with the Na+ cation vacancy is energetically more favorable than the structure with the Ca2+ cation vacancy. The calcium adsorption site has been shown to have a greater affinity for water than can be found in the case of the sodium site, demonstrating that affinity may not be considered a major factor controlling the release of cations from the glass to the solution.
An analysis of the long-term trends in the records of Friends of the Koala in north-east New South Wales: II. Post-release survival
Authors: Lunney Daniel , Cope Holly , Sonawane Indrie , Stalenberg Eleanor , Haering Ron 
Published: April 2022

Context Post-release monitoring of rehabilitated koalas is lacking, meaning that the long-term success rate is unknown. Aims We addressed the question: will a koala released from rehabilitation re-join the wild population and survive for months, if not years? Methods Using ear tag records as unique identifiers of individual koalas, we sifted the 31-year set of 5051 koala admission records (1989–2020) of a koala rehabilitation group, Friends of the Koala, in Lismore, north-east New South Wales for records of koalas that had returned to rehabilitation for a second, third or fourth time. Key results Of the 1771 koalas that were released, most (80%) had a coloured ear tag with a unique number. Of these koalas, 270 were admitted to rehabilitation two or more times and therefore represented an opportunity for post-release monitoring. Re-admission figures mostly fitted the pattern of first admissions, meaning that the released koalas had become part of the local koala population. Of the 270 koalas that were re-admitted into rehabilitation, 66% remained for more than 6 months in the wild, and 33% remained in the wild for more than 2 years. Conclusions We conclude that rehabilitated and released koalas can survive in the wild long-term, even though some koalas were re-admitted after a very brief period post-release. Implications The success of rehabilitation and release, as judged by re-admitted tagged koalas, is a more robust view of success for koala survival after rehabilitation than simply the proportion of released versus non-released koalas.