bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract LB562: L1-ATP8B1 exacerbated pathogenic high-level reduction-oxidation homeostasis to promote carcinogenesis in lung squamous cell carcinoma via affecting mitochondrial prohibitin 1 SUMOylation and increasing SLC1A5 expression
Authors: Yu Jinpu , Zhang Xiao , Zhang Rui , Sun Zeguo , Zhang Weijia 
Published: June 2022

Abstract
Long Interspersed Nuclear Element-1 (LINE-1, L1) plays important roles in carcinogenesis by forming L1 chimeric transcripts (LCTs) in somatic cells. However, the regulation and function of LCTs in lung squamous cell carcinoma (LUSC) remains unclear. Previously we identified L1-ATP8B1 as a prognostic LCT in LUSC. In this study we confirmed that L1-ATP8B1 was overexpressed in LUSC patients suffering from shorter survival and correlated with upregulation of LINE-1 ORF1, impaired expression of ATPase phospholipid transporting 8B1 (ATP8B1) and distinct mitochondria dysfunction in both TCGA dataset and TJMUCH cohort. Over-expression of L1-ATP8B1 in LUSC cell lines and xenograft-bearing mice promoted cell proliferation, migration and invasion in vitro and facilitated LUSC xenografts growth in vivo which could be attenuated by inhibiting LINE-1 activity via nevirapine (NVR). In addition, L1-ATP8B1 affected cardiolipin-dependent mitochondrial prohibitin 1 (PHB1) SUMOylation and increased GSH synthesis transporter protein SLC1A5 expression which produced excessive ROS and GSH to induce cellular high-level reduction-oxidation (REDOX) homeostasis. Interfering with REDOX homeostasis using antioxidant could inhibit L1-ATP8B1 overexpression, reverse L1-ATP8B1-related mitochondrial dysfunction and carcinogenesis. Furthermore, combined therapy of NVR and antioxidant dramatically abolished L1-ATP8B1-induced growth and metastasis of LUSC xenografts in vivo. In conclusion, we at the first time demonstrated metabolic disorder plays a vital role in LCTs-related carcinogenesis and L1-ATP8B1 promotes LUSC development and progression by exacerbating pathogenic high-level REDOX homeostasis, therefore L1-ATP8B1 is a promising prognostic biomarker and therapeutic target in LUSC.
Citation Format: Jinpu Yu, Xiao Zhang, Rui Zhang, Zeguo Sun, Weijia Zhang. L1-ATP8B1 exacerbated pathogenic high-level reduction-oxidation homeostasis to promote carcinogenesis in lung squamous cell carcinoma via affecting mitochondrial prohibitin 1 SUMOylation and increasing SLC1A5 expression [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr LB562.
Poland syndrome: a new classification system based on a retrospective analysis of 74 cases
Authors: Cabrera-Gonzalez Marisa , Gutierrez-de la Iglesia Diego , Fontecha Cesar G. , G. Garcia Rocio , Ortega-Centol Aritz , Perez-Lopez Laura M. 
Published: June 2022

The aim of this study was to present a classification of Poland syndrome deformities, which we hypothesized was simple, valid, reliable and useful in planning surgery. We retrospectively reviewed 74 patients with Poland syndrome treated in our centre between 1990 and 2019 (inclusive). The proposed classification comprised six types of ascending severity and was based on clinical and radiological findings. Agreement with the frequently used but more complex Catena classification was substantial (kappa 0.70), and interobserver reliability was close to perfect (kappa 0.87). The severity of the deformities according to this classification correlated well with the number of surgeries performed, except for Type 5, in which the result of surgery often is disappointing, and in Type 6, that is beyond the reach of surgical treatment. We conclude that this classification is useful in planning surgery and in advising parents on the surgical strategy. Level of evidence III
Long-stemmed Hemiarthroplasty with Cerclage Wiring for the Treatment of Split-Head Fractures of the Proximal Humerus with Metaphyseal Extension: A Report of 2 Cases
Authors: Panagopoulos A. , Solou K. , Kouzelis A. , Papagiannis S. , Tatani I. , Kokkalis Z. T. 
Published: June 2022

Introduction Complex fractures of the proximal humerus with splitting-head component and metaphyseal propagation are very rare injuries that are difficult to treat. Preservation of the humeral head is always considered except in cases with severe comminution and compromised vascularity where shoulder hemiarthroplasty is an alternative option. Case Report We present two male patients, 57- and 62-years-old who sustained such a complex proximal humeral fracture after a high-energy injury. They both managed with long-stemmed shoulder hemiarthroplasty and cerclage wiring of the metaphyseal area. They both demonstrated good clinical and radiological outcome at 32- and 24-months postoperatively. Conclusion We report the functional and radiological outcomes of two cases of a rare proximal humeral fracture's pattern - combination of splitting-head and metaphyseal comminution – that were managed with long-stemmed hemiarthroplasty and cerclage wire and demonstrated good midterm clinical outcome.
Conservative management of urethral prolapse in African girls: a report of 15 cases
Authors: Seck Ndeye Fatou , Ndoye Ndeye Aby , Zeng Florent Tshibwid A , Diop Mame Bounama , Cissé Lissoune , Wellé Ibrahima Bocar , Sagna Aloïse , Ngom Gabriel 
Published: June 2022

Abstract
Background
Urethral prolapse is a rare disease, mainly occurring in African prepubertal girls. Its etiology remains unclear; however, some risk factors have been reported. The diagnosis is made clinically. The treatment can be conservative or surgical.

Methods
We report our experience in the service of pediatric surgery at Albert Royer National Children’s Hospital Centre. We conducted a retrospective descriptive cross-sectional study, on which considered patients were managed for urethral prolapse in our service from 2014 to 2019.

Results
Our study has included 15 girls whose mean age was 4.08 years (1.17–10). Two risk factors (chronic cough and constipation) were found in 20%. Genital hemorrhage was the main symptom (73.3%), and suspicion of sexual abuse was documented in 13.3%. The clinical finding was classical (donut-shaped vulvar mass) in all patients. All patients underwent conservative management, which was successful in 73.3%. Patients with failed conservative management were treated surgically by resection of the prolapsed mucosa on a Foley catheter. No complication was reported after surgical treatment, and after a 13-month mean follow-up, no recurrence was reported in all patients.

Conclusions
Conservative management is an efficient option for urethral prolapse. It has the advantage of avoiding general anesthesia with its potential complications and restricted availability in sub-Saharan Africa. Surgical management should be reserved for failed conservative management.
Risk of selection bias assessment in the NINDS rt-PA stroke study
Authors: Garg Ravi , Mickenautsch Steffen 
Published: June 2022

Abstract
Objectives
The NINDS rt-PA Stroke Study is frequently cited in support of alteplase for acute ischemic stroke within 3 h of symptom onset. Multiple post-hoc reanalyses of this trial have been published to adjust for a baseline imbalance in stroke severity. We performed a risk of selection bias assessment and reanalyzed trial data to determine if the etiology of this baseline imbalance was more likely due to random chance or randomization errors.

Methods
A risk of selection bias assessment was conducted using signaling questions from the Cochrane Risk of Bias 2 (ROB 2) tool. Four sensitivity analyses were conducted on the trial data based on the randomization process: assessment of imbalances in allocation in unique strata; adherence to a pre-specified restriction on randomization between time strata at each randomization center; assessment of differences in baseline computed tomography (CT) results in unique strata; and comparison of baseline characteristics between allocation groups within each time strata. A multivariable logistic regression model was used to compare reported treatment effects with revised treatment effects after adjustment of baseline imbalances identified in the sensitivity analyses.

Results
Based on criteria from the ROB 2 tool, the risk of bias arising from the randomization process was high. Sensitivity analyses found 11 of 16 unique strata deviated from the expected 1:1 allocation ratio. Three randomization centers violated an apriori rule regarding a maximum difference in allocation between the time strata. Three unique strata had imbalances in baseline CT results that prognostically favored alteplase. Four imbalances in baseline characteristics were identified in the 91–180-min time stratum that all prognostically favored alteplase and were consistent with a larger alteplase treatment effect size compared to the 0–90-min time stratum. After adjustments for baseline imbalances, all reported treatment effects were reduced. Three out of seven originally positive reported results were revised to non-significant.

Conclusion
This risk of selection bias assessment revealed a high risk of selection bias in the NINDS rt-PA Stroke Study. Sensitivity analyses conducted based on the randomization process supported this assessment. Baseline imbalances in the trial were more likely due to randomization errors than random chance. Adjusted analyses accounting for baseline imbalances revealed a reduction in reported treatment effects supporting the presence of selection bias in the trial. Treatment decisions and guideline recommendations based on the original treatment effect reported in the NINDS rt-PA Stroke Study should be done cautiously.
The effect of HPV DNA and p16 status on the prognosis of patients with hypopharyngeal carcinoma: a meta-analysis
Authors: Shi Jinfeng , Wang Ling , Yao Nan , Sun Le , Hu Wenyu , Li Xiaotong , Yang Yixue , Wang Yusheng , Zhu Wei , Li Bo 
Published: June 2022

AbstractObjectiveTo evaluate whether the presence of human papillomavirus (HPV) DNA and p16 might be associated with better prognosis in patients with hypopharyngeal carcinoma (HPC), especially on overall survival (OS) and disease-free survival (DFS).MethodPubMed, the Cochrane Library, the Web of Science and EMBASE were searched from inception to April 2021 to search for HPV DNA- and p16-related prognostic articles on HPC. Meta-analysis was performed on the selected articles according to the inclusion and exclusion criteria. Publication bias was assessed for the included studies with Egger’s test. All studies were analyzed by using Stata 16.0 statistical software.ResultsA total of 18 studies were included, including 12 HPV DNA studies and 11 p16 studies. Meta-analysis showed that HPV DNA positivity was a strong prognostic factor for improved OS in patients with HPC, with a pooled hazard ratio (HR) of 0.61 (95% CI, 0.54–0.69), but there was no statistically significant difference in DFS (HR, 0.60; 95% CI, 0.31–1.16). Patients with p16-positive tumors had better OS (HR, 0.66; 95% CI, 0.49–0.89) and DFS (HR, 0.59; 95% CI, 0.44–0.78) than patients with p16-negative tumors.ConclusionsThis study suggests that the presence of HPV DNA leads to better OS in patients with HPC, and the presence of p16 also corresponds to better OS and DFS. Our results provide up-to-date evidence to clinicians and researchers. Larger studies adjusting for prognostic factors are needed in subsequent studies.
MiR-335 promotes corneal neovascularization by Targeting EGFR
Authors: Qian Jingjing , Yu Junbo , Zhu Xi , Liang Shu 
Published: June 2022

Abstract
Background
Corneal neovascularization (CRNV) is a severe threat to the vision of people. MicroRNA-335 (miR-335) has the function of facilitating angiogenesis. However, whether miR-335 regulates the progression of CRNV remains unclear.

Methods
The miR-335 expressions in CRNV rats induced by corneal suture and HUVECs induced by b-FGF were detected by quantitative real-time PCR. For the miR-335 function, wound healing and tube formation assays were performed. For the miR-335 mechanism, a dual-luciferase reporter gene assay was conducted. Besides, for the epidermal growth factor receptor (EGFR) function, Cell Counting Kit-8 and wound healing assays were performed. Meanwhile, the rescue assay was used to assess the miR-335/EGFR function in the migration and angiogenesis of b-FGF-treated HUVECs.

Results
Functionally, the miR-335 knockdown weakened the migration and angiogenesis of b-FGF-treated HUVECs, while the miR-335 overexpression showed an opposite trend. Mechanistically, miR-335 interacted with EGFR and negatively regulated the expression of EGFR. The rescue assay illustrated that miR-335 regulated the migration and angiogenesis of b-FGF-treated HUVECs through EGFR.

Conclusions
In general, our data confirmed that miR-335 facilitated the process of CRNV by targeting EGFR.
Radial distraction may reduce the incidence of ulnar-sided wrist pain in ulna-plus morphology intraoperatively following distal radius fractures fixation
Authors: Ma Hsuan-Hsiao , Huang Hui-Kuang , Yin Cheng-Yu , Huang Yi-Chao , Chang Ming-Chau , Wang Jung-Pan 
Published: June 2022

Abstract
Introduction
Fixed-angle plate fixation can be an effective treatment for distal radius fractures (DRFs). However, patients with existing ulnar positive variance might be at risk of developing symptoms of ulnar-sided wrist pain (USWP). Ulnar shortening osteotomy (USO) is one of the main treatment options for USWP. We hypothesized that a limited radial distraction at the fracture site at the time of surgery for DRF would be functionally equivalent to an indirect USO and that if this were done in a patient with an ulnar plus morphology it could potentially decrease the risk of USWP.

Methods
This retrospective study was conducted at a single institution and all the surgeries were performed by single surgeon. A total of 136 patients (92 women and 44 men) with a mean age of 55 years were enrolled with 57 patients in the distraction group (from 2014 to 2017) and 79 patients (from 2011 to 2013) in the non-distraction group. Patients were assessed USWP. Functional outcomes were assessed using the Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire, Visual Analogue Scale (VAS) for pain, grip strength, and range of motion for the wrist.

Results
The mean follow-up was 37.9 months (range, 28–61 months). The radiographs at postoperative 2-year follow-ups showed the mean ulnar positive variance was 1.3 mm (range, 1–2 mm) in the distraction group and 3.5 mm (range, 2-5 mm) in the non-distraction group. The average of the distraction length was 2.32 mm (range, 2–3 mm). At the 2-year follow-ups, USWP presented in 7% (four patients) in the distraction group, which was significantly less than the incidence of 28% (22 patients) in the non-distraction group. The distraction group exhibited significantly better DASH scores and grip strength and less subsequent ulnar-shortening osteotomy for ulnar-sided wrist pain.

Conclusions
The radial distraction procedure performed during DRFs fixation could possibly reduce the occurrence of postoperative USWP and improve the functional outcomes.

Level of evidence
Level III, Therapeutic.
Effect of the knee replacement surgery on activity level based on ActivPAL: a systematic review and meta-analysis study
Authors: Alfatafta Huda , Alfatafta Mahmoud , Onchonga David , Hammoud Sahar , Khatatbeh Haitham , Zhang Lu , Boncz Imre , Lohner Szimonetta , Molics Bálint 
Published: June 2022

Abstract
Background
The knee replacement (KR) surgery aims to restore the activity level and reduce the risk of experiencing disabilities. The outcomes of this surgery are evaluated mainly with subjective tools or low validity objective tools. However, the effect of the surgery on activity level using high validity objective accelerometer is still in question.

Methods
A systematic review and meta-analysis were conducted to evaluate the benefit of KR surgery alone to enhance physical activity recommendations based on high validity accelerometer. Two independent reviewers evaluated five electronic databases (Cochrane-Central-Register-of-Controlled Trials, EMBASE, PubMed, Web of Science, and Scopus) to find relative studies between January 2000 and October 2021. The quality assessments and risk of bias assessments were examined.

Results
Three articles were included with 202 participants (86 males, 116 females), with an average age of 64 years and an average 32 kg/m2 body mass index. The results found that the number of steps was significantly improved up to 36.35 and 45.5% after 6-months and 1-year of the surgery, respectively. However, these changes did not meet the recommended activity level guideline and could be related to the patients’ health status and their activity level before the surgery. No significant changes were seen in sedentary time, standing time, and upright time after 6-months and 1-year follow-ups. Heterogeneity among studies was low to moderate (0–63%).

Conclusion
Knee replacement surgery is an effective treatment for improving patients’ quality of life with severe knee injuries. However, various factors impact the success of surgical and achieving maximum benefit of the surgery. One factor, sedentary time, can be reduced by implementing pre-and post-surgery exercise or physical activity recommendations. Further studies are needed to understand the benefit of surgery with or without rehabilitation assessed using high validity monitors.
EGFR-targeted fluorescent imaging using the da Vinci® Firefly™ camera for gallbladder cancer
Authors: Choi Jung Ha , Kang Chang Moo , Park Jeong Youp 
Published: June 2022

Abstract
Background
Fluorescent imaging may aid with the precise diagnosis and treatment of patients with gallbladder cancer. In this study, we sought to demonstrate whether the da Vinci® surgical system and Firefly™ camera could detect EGFR-targeted fluorescent images in orthotopic mouse models of gallbladder cancer.

Methods
An orthotopic mouse model of gallbladder cancer was created by injecting NOZ gallbladder cancer cells mixed with Matrigel into the gallbladder. In vivo imaging of subcutaneous and orthotopic gallbladder tumors was performed after the injection of DyLight 650- or 800-conjugated EGFR antibody.

Results
Western blotting, flow cytometry, and confocal microscopy showed the presence of EGFR in NOZ cells, but not in HEK293 cells. Subcutaneous NOZ cell tumors fluoresced after injection with fluorescent EGFR antibody, but subcutaneous HEK293 tumors did not. Fluorescent EGFR antibody made orthotopic NOZ tumors fluoresce, with an intensity stronger than that in the surrounding normal tissues. Histochemical examination confirmed the location of the tumors inside the gallbladder and adjacent liver parenchyma. Fluorescent signal was also detected in orthotopic gallbladder tumors with Firefly™ camera.

Conclusion
Our study showed that fluorescent EGFR antibodies and the Firefly camera in the da Vinci system can detect fluorescing gallbladder tumors, which demonstrates their potential use for molecular imaging-based prevision surgery in the near future.
Effect of shifting cultivation and fallow on soil quality index in Mokokchung district, Nagaland, India
Authors: Temjen Wati , Singh Maibam Romeo , Ajungla Tali 
Published: June 2022

Abstract
Background
Shifting cultivation is a major agriculture practice in the Nagaland state of India. This study examines the effect of shifting cultivation and the length of the fallow period on soil quality index (SQI). Four sites were selected for the study, viz., a shifting cultivation site (SCS), a 3-year-old fallow land (FL-3), a 7-year-old fallow land (FL-7), and a 12-year-old fallow land (FL-12). Soil parameters were recorded seasonally and SQI was calculated from the minimum data set.

Results
With the increase in the fallow period, the values of conductivity, soil organic carbon, available nitrogen, available phosphorus, exchangeable potassium, moisture, clay, and cation exchange capacity of soil increased. Meanwhile, soil pH and bulk density decreased with fallow duration. The additive SQIa values were in the order SCS < FL-3 < FL-12 < FL-7; meanwhile, the weighted SQIw values were in the order SCS < FL-3 < FL-7 < FL-12. It is also observed that the SQI value decreases with the increase in soil depth under both the weighted and additive indexes. SCS with the lowest SQI value reflects the reduced soil organic carbon (SOC) and macronutrients. Increased SOC levels in site FL-12 (2.88–3.94%) may be one reason for its higher SQI value.

Conclusions
Our study highlights that unsustainable practices of shifting cultivation and reduction in the fallow period negatively affect soil quality. Furthermore, the study also recommends the use of the weighted method of SQI as it agrees with the reports of land use causing alteration in the soil quality. Our findings may be utilized to quickly access and disseminate information to the stakeholders and aid in constructing local soil quality index maps of the region. There is an urgent need for a rapid, cost and resource-efficient soil quality assessment and SQI may be one tool that achieves this goal.
Alone against the danger: a study of the routine precautions taken by voluntary sex workers to avoid victimisation
Authors: Molnar Lorena , Aebi Marcelo F. 
Published: June 2022

AbstractThis article explores the routine precautions taken by sex workers (SW) in Switzerland, a country in which sex work is a legal activity. It is based on approximately 1100 h of non-systematic participant observation spread over 18 months and 14 semi-structured interviews with indoor and outdoor SW. The findings show that SW use a series of routine precautions that overlap with the situational prevention techniques for increasing perpetrators’ efforts or their perception of the risk of offending, reducing the rewards of the crime, and decreasing the provocations and perpetrators’ excuses. Future tests of the efficacy of these routine precautions could help developing specific situational crime prevention techniques for deterring offences against SW.
Dispatches to the Dead: Delegation, Consumption, and Mischievous Pleasure (Thinking with Robert Pfaller in the So-called Present)
Authors: Steintrager James A. , Chow Rey 
Published: June 2022

AbstractFor the past twenty years or so, the Austrian cultural theorist Robert Pfaller has been positing and analyzing the concepts of “interpassivity” (as opposed to interactivity) and the delegation of enjoyment both in sacred rituals and in relation to various media of reproduction (such as video recorders and photocopiers). Steintrager and Chow argue that Pfaller's insights into delegation as a key and understudied feature of media ecologies are particularly relevant today, as delegation of various activities has intensified. However, the latest media and technologies, which gather so much information about us that we end up in a state of informational debt, pose a challenge to his thesis that delegation is an unconscious strategy for seeking relief from the onslaught of images, commodities, and information.