bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adición de polvo de vidrio reciclado en la estabilización de suelos en el AA.HH. Villa Hermosa, Nuevo Chimbote (Perú)
Authors: Haro Marchena Luis Miguel , Cerna Vásquez Marco Antonio , Valdivieso Velarde Alan Yordan 
Published: May 2022

El estado físico de la zona de estudio a nivel de terreno natural fue encontrado en condiciones de desgaste por la constante transitabilidad vehicular. Por tal motivo de busco mejorar la calidad del suelo del AA. HH Villa Hermosa, Nuevo Chimbote – Perú. Se realizó los estudios para evaluar la resistencia a la compresión mediante los ensayos de campo como: granulometría, humedad natural, Proctor modificado y CBR; el cual dio como resultado un promedio de 18.24 % en valor de CBR al (95%) en terreno natural y al ser mejorado con el aditivo de polvo de vidrio reciclado al 6% se obtuvo un promedio de 28.46 % y al 8% un promedio de 25.78%. Como se observa el mejor resultado óptimo fue al 6% el cual muestra una mejora de 56% respecto al terreno natural, el cual se podría utilizar con porcentajes menores para obtener nuevos resultados.
Aprovechamiento sostenible para una especie de palma utilizada como material estructural para edificaciones de dos pisos ubicadas en Quibdó (Chocó)
Authors: Hinestroza Serna Antonio Manuel 
Published: May 2022

Quibdó, capital del departamento del Choco, por su biodiversidad la ubica como una de las zonas con exuberancia en especies forestales de la región, contribuyo a establecer e identificar las palmas adecuadas para la construcción de edificaciones por tal motivo se visitó uno de los corregimientos del municipio de Quibdó, Tutunendo, donde se encuentran palmas adecuadas para la construcción de edificaciones, una de ellas es la Zancona (Socratea exorrhiza) la cual es convertida por medio de un proceso tradicional en esterilla y posteriormente es puesta al sol para garantizar su durabilidad y resistencia en consecuencia se estableció la combinación de la esterilla con el concreto para ser empleado este material nuevo en construcción de edificaciones arquitectónicas, se llevaron entonces unas muestras o probetas a un laboratorio logrando una resistencia adecuada para ser utilizado en el proceso constructivo del entrepiso para edificaciones de dos pisos lo cual contribuirá a disminuir costos.
La biblioteca como infraestructura cultural y herramienta para la puesta en valor del patrimonio construido
Authors: De Andrés de Vicente Javier , De Vicente García Remedios 
Published: May 2022

Con la irrupción del mundo digital, que en cierto modo ha supuesto una disminución en el número de usuarios que acuden a las bibliotecas, estas se ven obligadas a replantear su papel para conectar y atraer nuevos usuarios, pasando de ser espacios donde buscar información a ser lugares en los que encontrar experiencias.
Será en esta condición fenomenológica de la biblioteca como espacio experiencial donde residirá una de las claves para redefinir los espacios físicos de las bibliotecas del s. XXI. Desde este enfoque, la recuperación de edificios patrimoniales de muy diversa índole como espacios para bibliotecas, ofrece una experiencia enriquecedora al entender los contenedores patrimoniales como fuente primaria de conocimiento cultural.
De igual forma, la atmósfera híbrida que en ellas se genera entre lo nuevo y lo viejo, así como la tensión que se produce entre los espacios originales y los nuevos usos, favorecerá la readaptabilidad del conjunto a posibles actividades futuras, convirtiendo estas instituciones patrimoniales en un marco ideal para cobijar las bibliotecas contemporáneas del s. XXI.
Patient Views on Quality of Life and Hospital Care: Results From a Qualitative Study Among Vietnamese Patients With Diabetes
Authors: Tri Mai Trong , Khue Nguyen Thy , Khoa Vo Tuan , Goto Aya 
Published: May 2022

ObjectivesThis study aimed to fill the gap between Vietnamese diabetic patients' needs and care through a qualitative study asking about their experiences with diabetes and quality of care.MethodsInterviews with five diabetic patients were conducted at a tertiary general hospital located in southern Vietnam. The transcribed data were first subjected to quantitative text analysis using KH Coder to identify major categories of frequently used words, followed by a qualitative analysis of selected cases using the Steps for Coding and Theorization (SCAT) method.ResultsThe major categories of frequently used words were chronic health conditions, services, facilities, insurance, patient-doctor communication, and medication. SCAT analysis of three selected cases identified six themes: “Disregarding the disease at the early stage,” “Fear of complications,” “Satisfaction with hospital services and medical staff,” “Insurance-related problems,” “Long waiting times,” and “Communication barriers between patients and doctors.” Patients were satisfied with improved hospital facilities and services; however, the overloading of one hospital led to long waiting times and communication difficulties with doctors. Difficulties with health insurance were also observed, and patients were rather passive in disease management and needed to be empowered through improved communication with doctors and other care providers.ConclusionThese findings from our trial of introducing a qualitative study into service evaluation suggest that listening to patients can help health providers learn their perspectives and be more responsive to their needs.
Benefits of Newborn Screening for Vitamin D-Dependant Rickets Type 1A in a Founder Population
Authors: Fortin Carol-Ann , Girard Lysanne , Bonenfant Chloé , Leblanc Josianne , Cruz-Marino Tania , Blackburn Marie-Eve , Desmeules Mathieu , Bouchard Luigi 
Published: May 2022

BackgroundVitamin D-dependant rickets type 1A (VDDR1A) is a rare autosomal recessive disorder caused by pathogenic variants in the CYP27B1 gene. This gene is essential for vitamin D activation. Although VDDR1A is a rare condition worldwide, its prevalence is high in the Saguenay-Lac-Saint-Jean (SLSJ) region due to a founder effect. Daily intake of calcitriol before the onset of clinical manifestations can prevent them in affected children.MethodsA genetic screening test was developed and validated for the CYP27B1 gene c.262del pathogenic variant. Newborn screening was implemented in the SLSJ region for this variant, and the feasibility and acceptability were assessed. Sixteen medical records of children affected with VDDR1A were reviewed to document the consequences of the disease at diagnosis.ResultsA total of 2000 newborns were tested for VDDR1A. Most families (96.5%) accepted the genetic test. We found a carrier rate of 1/29 for the c.262delG variant in our cohort, which is suggestive of a founder effect. We identified one child affected with VDDR1A and treatment was initiated before the onset of clinical manifestations. On average, children with VDDR1A were diagnosed at 13.8 ± 5 months of age, they had a significant failure to thrive at diagnosis, among other harmful health consequences.ConclusionOur study showed that in our population, the newborn genetic screening program is safe and feasible, it has high acceptability, and it is efficient to identify affected children. VDDR1A health consequences can be prevented by early initiation of treatment. Therefore, screening programs should be available for populations where it is deemed as beneficial from a public health perspective.
Differences in the Establishment of Gut Microbiota and Metabolome Characteristics Between Balb/c and C57BL/6J Mice After Proton Irradiation
Authors: Li Yuchen , Sui Li , Zhao Hongling , Zhang Wen , Gao Lei , Hu Weixiang , Song Man , Liu Xiaochang , Kong Fuquan , Gong Yihao , Wang Qiaojuan , Guan Hua , Zhou Pingkun 
Published: May 2022

Although proton irradiation is ubiquitous in outer space as well as in the treatment of human diseases, its effects remain largely unclear. This work aimed to investigate and compare the composition of gut microbiota composition of mice in different species exposed to high-dose radiation. Male Balb/c mice and C57BL/6J mice were irradiated at a high dose (5Gy). Fecal specimens before and after irradiation were subjected to high-throughput sequencing (HTS) for the amplification of 16S rRNA gene sequences. We observed substantial changes in gut microbial composition among mice irradiated at high doses compared to non-irradiated controls. The changes included both the alpha and beta diversities. Furthermore, there were 11 distinct alterations in the irradiation group compared to the non-radiation control, including the families Muribaculaceae, Ruminococcaceae, Lactobacillus, Lachnospiraceae_NK4A136, Bacteroides, Alistipes, Clostridiales, Muribaculum, and Alloprevotella. Such alterations in the gut microbiome were accompanied by alterations in metabolite abundances, while at the metabolic level, 32 metabolites were likely to be potential biomarkers. Some alterations may have a positive effect on the repair of intestinal damage. Simultaneously, metabolites were predicted to involve multiple signal pathways, such as Urea Cycle, Ammonia Recycling, Alpha Linolenic Acid and Linoleic Acid Metabolism, Ketone Body Metabolism, Aspartate Metabolism, Phenylacetate Metabolism, Malate-Aspartate Shuttle, Arginine and Proline Metabolism and Carnitine Synthesis. Metabolites produced by proton irradiation in the microbial region play a positive role in repairing damage, making this area worthy of further experimental exploration. The present work offers an analytical and theoretical foundation to investigate how proton radiation affects the treatment of human diseases and identifies potential biomarkers to address the adverse effects of radiation.ImportanceThe space radiation environment is extremely complex, protons radiation is still the main component of space radiation and play an important role in space radiation. We proposed for the first time to compare the feces of Balb/c and C57BL/6J mice to study the changes of intestinal flora before and after proton irradiation. However, the effect of proton irradiation on the gut microbiome of both types of mice has not been previously demonstrated. After proton irradiation in two kinds of mice, we found that the characteristics of intestinal microbiome were related to the repair of intestinal injury, and some metabolites played a positive role in the repair of intestinal injury.
Handwriting Declines With Human Aging: A Machine Learning Study
Authors: Asci Francesco , Scardapane Simone , Zampogna Alessandro , D’Onofrio Valentina , Testa Lucia , Patera Martina , Falletti Marco , Marsili Luca , Suppa Antonio 
Published: May 2022

BackgroundHandwriting is an acquired complex cognitive and motor skill resulting from the activation of a widespread brain network. Handwriting therefore may provide biologically relevant information on health status. Also, handwriting can be collected easily in an ecological scenario, through safe, cheap, and largely available tools. Hence, objective handwriting analysis through artificial intelligence would represent an innovative strategy for telemedicine purposes in healthy subjects and people affected by neurological disorders.Materials and MethodsOne-hundred and fifty-six healthy subjects (61 males; 49.6 ± 20.4 years) were enrolled and divided according to age into three subgroups: Younger adults (YA), middle-aged adults (MA), and older adults (OA). Participants performed an ecological handwriting task that was digitalized through smartphones. Data underwent the DBNet algorithm for measuring and comparing the average stroke sizes in the three groups. A convolutional neural network (CNN) was also used to classify handwriting samples. Lastly, receiver operating characteristic (ROC) curves and sensitivity, specificity, positive, negative predictive values (PPV, NPV), accuracy and area under the curve (AUC) were calculated to report the performance of the algorithm.ResultsStroke sizes were significantly smaller in OA than in MA and YA. The CNN classifier objectively discriminated YA vs. OA (sensitivity = 82%, specificity = 80%, PPV = 78%, NPV = 79%, accuracy = 77%, and AUC = 0.84), MA vs. OA (sensitivity = 84%, specificity = 56%, PPV = 78%, NPV = 73%, accuracy = 74%, and AUC = 0.7), and YA vs. MA (sensitivity = 75%, specificity = 82%, PPV = 79%, NPV = 83%, accuracy = 79%, and AUC = 0.83).DiscussionHandwriting progressively declines with human aging. The effect of physiological aging on handwriting abilities can be detected remotely and objectively by using machine learning algorithms.
ATP6V0C Is Associated With Febrile Seizures and Epilepsy With Febrile Seizures Plus
Authors: Tian Yang , Zhai Qiong-Xiang , Li Xiao-Jing , Shi Zhen , Cheng Chuan-Fang , Fan Cui-Xia , Tang Bin , Zhang Ying , He Yun-Yan , Li Wen-Bin , Luo Sheng , Hou Chi , Chen Wen-Xiong , Liao Wei-Ping , Wang Jie 
Published: May 2022

PurposeTo identify novel genetic causes of febrile seizures (FS) and epilepsy with febrile seizures plus (EFS+).MethodsWe performed whole-exome sequencing in a cohort of 32 families, in which at least two individuals were affected by FS or EFS+. The probands, their parents, and available family members were recruited to ascertain whether the genetic variants were co-segregation. Genes with repetitively identified variants with segregations were selected for further studies to define the gene-disease association.ResultsWe identified two heterozygous ATP6V0C mutations (c.64G &gt; A/p.Ala22Thr and c.361_373del/p.Thr121Profs*7) in two unrelated families with six individuals affected by FS or EFS+. The missense mutation was located in the proteolipid c-ring that cooperated with a-subunit forming the hemichannel for proton transferring. It also affected the hydrogen bonds with surround residues and the protein stability, implying a damaging effect. The frameshift mutation resulted in a loss of function by yielding a premature termination of 28 residues at the C-terminus of the protein. The frequencies of ATP6V0C mutations identified in this cohort were significantly higher than that in the control populations. All the six affected individuals suffered from their first FS at the age of 7–8 months. The two probands later manifested afebrile seizures including myoclonic seizures that responded well to lamotrigine. They all displayed favorable outcomes without intellectual or developmental abnormalities, although afebrile seizures or frequent seizures occurred.ConclusionThis study suggests that ATP6V0C is potentially a candidate pathogenic gene of FS and EFS+. Screening for ATP6V0C mutations would help differentiating patients with Dravet syndrome caused by SCN1A mutations, which presented similar clinical manifestation but different responses to antiepileptic treatment.
Genetic Analysis and Functional Study of a Pedigree With Bruck Syndrome Caused by PLOD2 Variant
Authors: Wang Ruo-li , Ruan Dan-dan , Hu Ya-nan , Gan Yu-mian , Lin Xin-fu , Fang Zhu-ting , Liao Li-sheng , Tang Fa-qiang , He Wu-bing , Luo Jie-wei 
Published: May 2022

BackgroundBruck syndrome (BS) is a rare autosomal recessive inherited osteogenesis imperfecta disease characterized by increased bone fragility and joint contracture. The pathogenic gene of type I BS is FKBPl0, whereas that of type II BS is PLOD2. No significant difference has been found in the clinical phenotype between the two types of BS. In this study, we performed genetic analysis of a BS pedigree caused by PLOD2 variant and studied the corresponding cellular function.MethodsSerum biochemistry, parathyroid hormone (PTH), 25-hydroxyvitamin D [25-(OH) D], osteocalcin, and 24-h urinary calcium levels of a family member with BS was assessed. The genes of the proband were analyzed by second-generation sequencing and exon capture techniques. Sanger sequencing was also performed for the suspected responsible variant of the family member. Wild- and variant-type lentivirus plasmids were constructed by gene cloning and transfected into HEK293T cells. Cell function was verified by real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence detection.ResultsIn this pedigree, the proband was found to have a homozygous variant c.1856G &gt; A (p.Arg619His) in exon 17 of PLOD2 (NM_182943.3). His consanguineous parents and sisters were p.Arg619His heterozygous carriers. The mRNA expression of PLOD2 in the constructed p.Arg619His variant cells was significantly upregulated, while the expression of PLOD2 and collagen I protein in the cell lysate was significantly downregulated. Immunofluorescence revealed that the wild-type PLOD2 was mainly located in the cytoplasm, and the expression of the PLOD2 protein after c.1856G &gt; A variant was significantly downregulated, with almost no expression, aligning with the western blot results. The serum sodium, potassium, calcium, phosphorus, magnesium, alkaline phosphatase, PTH, 25-(OH) D, osteocalcin, and 24 h urinary calcium levels of the proband, his parents, and sisters were normal.ConclusionThrough gene and cell function analyses, PLOD2 Arg619His missense variant was preliminarily confirmed to cause BS by reducing protein expression.
Examining the Constructs of Burnout, Compassion Fatigue, Secondary Traumatic Stress in Physicians Using Factor Analyses
Authors: Ahmed Fadwa , Baruch Jay , Armstrong Paul 
Published: May 2022

BackgroundAdverse affective experiences have been well-documented in healthcare providers. Research describes them under a variety of terms, including burnout, secondary traumatic stress (STS), and compassion fatigue (CF). The present study evaluates conflicting models of STS, CF, and burnout constructs in physicians.MethodsSurveys were mailed to all allopathic physicians with active Rhode Island medical licenses. Three hundred and seventy-five complete responses were received. The survey included common measures of STS, CF, and burnout. Confirmatory Factor Analysis (CFA) was used to evaluate discriminant validity of the three constructs and test 5 a priori (1-, 2-, and 3-factor) theoretical models, and Exploratory Factor Analysis (EFA) was planned assess underlying factor structure in the case that CFA did not provide evidence supporting any existing model.ResultsBy CFA, all five a priori models of burnout, CF, and STS fail to demonstrate adequate model fit (Standardized Root Mean Square Residual &gt;0.10, Tucker-Lewis Index &lt;0.90). EFA with parallel analysis extracts four factors underlying the three burnout, STS, and CF measures. The four factors describe 54.3% of variance and can be described as (1) depressive mood; (2) primary traumatic stress-like symptoms; (3) responses to patients' trauma; and (4) sleep disturbances.ConclusionIn spite of abundant discussion surrounding burnout, CF, and STS in physicians, measures of these constructs did not uphold their theoretical factor structures in the present study. Future research might explore other constructs and measures that may describe adverse affective physician experiences.
Introducing a New Technique for Fascial Closure to Avoid Renal Allograft Compartment Syndrome in Pediatric Recipients: The Use of Tutoplast® Fascia Lata
Authors: Bañuelos Marco Beatriz , Bergel Berenice , Geppert Tamara , Müller Dominik , Lingnau Anja 
Published: May 2022

IntroductionRenal allograft compartment syndrome (RACS) is a complication that infrequently occurs after renal transplantation. Tight muscle closure may lead to RACS due to compression of renal parenchyma or kinking of the renal vessels. Many techniques have been proposed in an attempt to achieve tension-free closure, which can be specially challenging in child recipients. We present our experience with Tutoplast® Fascia Lata (RTI Surgical Tutogen Medical GmbH Industriestrasse 6, 91077 Neunkirchen am Brand, Germany) closure.MethodsAll pediatric patients who underwent renal transplantation in our center between 2012 and 2021 were reviewed. Eight patients with Tutoplast® Fascia Lata placed at the time of initial transplantation were identified. Donor and recipient characteristics, Doppler ultrasound findings, and overall patient and graft survival rates were analyzed.ResultsDoppler ultrasound was performed intra-operatively after abdominal wall closure. If any sign of vascular compromise was seen, the abdominal wall was opened and the graft was revised. The Tutoplast® Fascia Lata implant was used to perform tension-free fascia closure and, afterwards, a Doppler ultrasound was performed to confirm the optimal renal artery perfusion and venous patency. Three of the renal transplantations were from a cadaver donor, with two of them en bloc. Living donor transplantation was performed in four cases. Among which, there was a case of auto-transplantation due to bilateral renal artery stenosis. None of the patients presented any complications of either short or long term that was derived from the abdominal closure with Tutoplast® Fascia Lata. There was also no record of graft failure till datum.ConclusionsRestricted volume of the recipient pelvic cavity and the size discrepancy between the recipient pelvic cavity space and the donor adult kidney may lead to RACS. Other situations that occur more infrequently, i.e., as en bloc or auto-transplantation, are prone to suffer the same problem. Tutoplast® Fascia Lata is a safe option for these patients.
Clinical Efficacy of Endoscopic-Assisted Resection of Single-Segment Ossification of the Posterior Longitudinal Ligament in the Treatment of Thoracic Spinal Stenosis
Authors: Li Xingchen , Huang Honghan , Zheng Zhong , Liu Yunxuan , Wei Guicai , Chen Xiaoxin , Xu Yusheng 
Published: May 2022

ObjectiveTo explore the clinical efficacy, characteristics and safety of endoscopic-assisted resection of single-segment posterior longitudinal ligament ossification in the treatment of thoracic spinal stenosis (TSS).MethodFifteen TSS patients, including 6 males and 9 females aged 43–70 years treated with endoscopic-assisted resection of single-segment posterior longitudinal ligament ossification through the transfacet joint approach by our team from November 2016 to June 2020 were retrospectively analyzed. The operation time, intraoperative blood loss, and postoperative complications were recorded. The VAS score, ODI and JOA score (full score, 11 points) were recorded before the operation, after the operation and at the last follow-up to evaluate the clinical efficacy and calculate the improvement rate.ResultsThe ventral side of the spinal cord was decompressed in all patients, providing improvements in neurological symptoms and significant pain relief. The mean follow-up time was 20.27 ± 3.87 months. Mean operation time, intraoperative blood loss, and hospitalization time were found to be 84.80 ± 13.23 min, 36.33 ± 7.41 mL, 5.13 ± 1.02 days; respectively.The JOA score at the last follow-up was 8.6 ± 1.25, which was significantly better than the preoperative (5.53 ± 1.20) and postoperative (6.87 ± 1.31) scores (p &lt; 0.05). The mean JOA score improvement rate was 56.5 ± 18.00%. The JOA score improvement rate classification at the last follow-up was excellent in 3 cases, good in 8 cases, effective in 3 cases, and no change in 1 case; for an effective rate of 93.33%. The VAS score significantly decreased from 6.67 ± 1.01 preoperatively to 3.47 ± 0.88 postoperatively and 1.73 ± 0.67 at the last follow-up (p &lt; 0.05). The ODI significantly decreased from 72.07 ± 6.08 preoperatively to 45.93 ± 5.01 postoperatively and 12.53 ± 2.33 at the last follow-up (p &lt; 0.05). Dural rupture occurred in 2 patients during the operation; 1 patient experienced neck discomfort during the operation, which was considered to be caused by high fluid pressure and was relieved by massage and by lowering the height of the irrigation fluid. No cases of cerebrospinal fluid leakage, wound infection or other complications occurred.ConclusionEndoscopic-assisted resection of posterior longitudinal ligament ossification through the facet joint approach is a safe and effective method for the treatment of TSS.