bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supramolecular Rotaxane‐Based Multi‐Modal Probes for Cancer Biomarker Imaging**
Authors: d'Orchymont Faustine , Holland Jason P. 
Published: May 2022

AbstractMechanically interlocked molecules present opportunities to construct therapeutic drugs and diagnostic imaging agents but harnessing supramolecular chemistry to make biologically active probes in water is a challenge. Here, we describe a rotaxane‐based approach to synthesise radiolabelled proteins and peptides for molecular imaging of cancer biomarkers in vivo. Host–guest chemistry using β‐cyclodextrin‐ and cucurbit[6]uril‐catalysed cooperative capture synthesis produced gallium‐68 or zirconium‐89 radiolabelled metallo[4]rotaxanes. Photochemical conjugation to trastuzumab led to a viable positron emission tomography (PET) radiotracer. The rotaxane architecture can be tuned to accommodate different radiometal ion complexes, other protein‐ or peptide‐based drugs, and fluorophores for optical detection. This technology provides a platform to explore how mechanical bonding can improve drug delivery, enhance tumour specificity, control radiotracer pharmacokinetics, and reduce dosimetry.
Foto‐Induzierte Ruthenium‐Katalysierte Doppel C(sp2)−H/C(sp3)−H Funktionalisierungen durch Radikalübertragungen
Authors: Wang Yulei , Chen Shan , Chen Xinran , Zangarelli Agnese , Ackermann Lutz 
Published: May 2022

AbstractDistale C(sp2)−H‐ und C(sp3)−H‐Funktionalisierungen haben sich kürzlich als schrittökonomische Werkzeuge für die molekulare Synthese entwickelt. Obwohl der Aufbau von C(sp2)−C(sp3)‐Bindungen von grundlegender Bedeutung ist, hat sich ihre Bildung durch doppelte C(sp2)−H/C(sp3)−H‐Aktivierung als schwierig erwiesen. Durch die Kombination der Ruthenium‐katalysierten meta‐C(sp2)−H‐Funktionalisierung durch einen aliphatischen Wasserstoffatomtransfer (HAT)‐Prozess beschreiben wir hier zweifach C(sp2)−H/C(sp3)−H‐Funktionalisierungen durch foto‐induzierte Ruthenium‐vermittelte Radikalübertragungen. Somit wurden meta‐C(sp2)−H‐Arenbindungen und distale C(sp3)−H‐Alkanbindungen durch einen einzigen Katalysator aktiviert. Dieser Prozess wurde bei Raumtemperatur unter der Bestrahlung mit sichtbarem Licht erreicht – bemerkenswerterweise ohne exogene Photokatalysatoren. Experimentelle und rechnerische Studien deckten die zugrunde liegenden mechanistischen Schritte auf, die die ortho‐C−H‐Aktivierung, Einzelelektronentransfer (SET), 1,n‐HAT (n=5–7) und σ‐Aktivierung durch einen einzigen Ruthenium(II)‐Katalysator umfassten.
Toughness enhancement of polyamide 6,12 with intermolecular hydrogen bonding with 3‐pentadecylphenol
Authors: Lu Jing , Jalali Amirjalal , Yao Jianqi , Ma Qin , Yin Jiajie , Zhang Ruiyan , Luo Faliang 
Published: May 2022

AbstractDue to the brittle performance of polyamide 6,12 (PA6,12) in high impact environment, we report a facile, efficient, and scalable method to toughen PA6,12 by establishing intermolecular hydrogen bonding (H‐bonding) interaction with 3‐pentadecylphenol (PDP). Interestingly, the intermolecular H‐bonding interaction obviously hindered PA crystallization, which was evidenced by decrease of crystalline temperature from 186.8 to 175.8°C and prolong of crystallization half‐time from 0.27 to 0.37 min, when the amount of PDP was increased to 30 wt%. Consequently, the toughness of the PA6,12/PDP composites containing 30 wt% PDP was improved and their notched impact strength reached to 17.5 kJ m−2, about three times higher than that of the neat PA6,12. It was deduced that both plasticizing effect and intermolecular H‐bonding interaction played critical roles in PA6,12 resultant composites toughness, which opens a new perspective of PA6,12 toughening for a wide range of applications.
Exploring the linkages of green transformational leadership, organizational green learning, and radical green innovation
Authors: Cui Rixiao , Wang Juanru , Zhou Cong 
Published: May 2022

AbstractRadical green innovation is the necessary way for countries and firms to achieve sustainable development. Although the influencing factors of green innovation have attracted extensive attention, there is little research on the antecedents of radical green innovation. Drawing on organizational learning theory and attention‐based view, this study proposes R‐I ratio to measure the configuration of exploratory green learning and exploitative green learning, then analyzes the relationships among green transformational leadership, R‐I ratio and radical green innovation, and examines the moderating effects of green R&D investment and environmental regulatory pressure. Based on a sample of 243 manufacturing firms in China's strategic emerging industries, the empirical results reveal that green transformational leadership promotes R‐I ratio, and R‐I ratio has inverted U‐shaped relationship with radical green innovation. The results also find that green R&D investment plays U‐shaped moderating role in the relationship between green transformational leadership and R‐I ratio, and environmental regulatory pressure positively moderates the relationship between green transformational leadership and R‐I ratio. The study not only reveals the relationships of green transformational leadership, organizational green learning and radical green innovation, but also provides theoretical guidance and management practice for manufacturing firms and government to promote radical green innovation.
Presentation of preclinical gastrointestinal anatomy via laparoscopic simulation
Authors: McCumber Travis L. , Mott Justin L. , Merani Shaheed , Rochling Fedja A. 
Published: May 2022

AbstractIn this report, the authors examine the integration of teaching anatomical science with clinical implications in minimally invasive surgery. The authors hypothesized that implementation of integrated laparoscopic simulation during undergraduate medical education would improve student learning of anatomical structures from in situ, laparoscopic orientations; and subsequently improve student preparation for clinical rotations and clerkships. During the fall of 2020 and 2021, 260 (130 students/year) second year medical students at the University of Nebraska Medical Center participated in a six‐week gastrointestinal curriculum. Following a traditional anatomy dissection experience, students completed a laparoscopic event consisting of narrated laparoscopic videos and hands‐on laparoscopic simulation. To examine the integrated curricular event, outcome measures focused on technical performance using grasping forceps, anatomical knowledge, and perception of the educational innovation. Outcomes were analyzed via timed performance and a pre and post assessment that was designed to assess student anatomical knowledge and perception. Completion of the technical performance assessment ranged from 1 min, 17 s to 6 min. Student knowledge of anatomical structures from in situ, laparoscopic orientations following the laparoscopic simulation sessions was significantly improved (53.3% pre vs 81.0% post), and almost all students (98.9%) agreed that the simulation sessions improved their understanding of laparoscopic anatomy and procedures. This report demonstrates the implementation of a multidisciplinary, integrated simulation that satisfied basic science anatomy teaching objectives, while enhancing student enthusiasm for the content. Future studies will examine the subsequent impact of the innovation on student preparedness for clinical rotations and clerkships.
Xanthones from Calophyllum Polyanthum Wallich ex Choisy with CYP1 Enzymes Inhibitory Activity
Authors: Cao Ting‐Ting , Huang Ruo‐Yue , Li Xu , Yang Teng‐Yun , Xie Hui‐Ding , Shen Yun‐Heng , Li Fei , Li Xian 
Published: May 2022

AbstractThree new xanthone compounds, 1,3,5‐trihydroxy‐2‐(2‐hydroxy‐3‐methylbut‐3‐enyl)‐4‐(3‐methylbut‐2‐enyl)xanthone (1), toxyloxanthone E (2), dehydrocycloguanandin B (3) along with 15 known xanthones (4–18) were isolated from the aerial parts of Calophyllum polyanthum Wall. ex Choisy. Their structures were fully characterised using spectroscopic data, as well as comparison with the previous literature data. All isolated compounds had inhibitory effects against CYP1A1, CYP1A2 and CYP1B1 enzymes at working concentration of 10 μM, 1 μM and 10 μM, respectively. Among them, compounds 10, 11, and 12 exhibited better CYP1A2 enzyme inhibitory effects than that of the positive control α‐naphthoflavone, with 51.05 %, 56.82 % and 44.93 % inhibition, respectively.
Context‐Dependence of the Reactivity of Cysteine and Lysine Residues
Authors: Boll Linus B. , Raines Ronald T. 
Published: May 2022

AbstractThe S‐alkylation of Cys residues with a maleimide and the Nϵ‐acylation of Lys residues with an N‐hydroxysuccinimide (NHS) ester are common methods for bioconjugation. Using Cys and Lys derivatives as proxies, we assessed differences in reactivity depending on the position of Cys or Lys in a protein sequence. We find that Cys position is exploitable to improve site‐selectivity in maleimide‐based modifications. Reactivity decreases substantially in the order N‐terminal>in‐chain>C‐terminal Cys due to modulation of sulfhydryl pKa by the α‐ammonium and carboxylate groups at the termini. A lower pKa value yields a larger fraction thiolate, which promotes selectivity while somewhat decreasing thiolate nucleophilicity in accord with
=0.41. Lowering pH and salt concentration enhances selectivity still further. In contrast, differences in the reactivity of Lys towards an NHS ester were modest due to an appreciable decrease in amino group nucleophilicity with a lower pKa of its conjugate acid. Hence, site‐selective Lys modification protocols will require electrophiles other than NHS esters.
Growing teratoma syndrome: Two case reports
Authors: Shrestha Anju , Dhakal Hari , Pandey Sirish Raj , Amatya Kapendra Shekhar , Shrestha Sudip , Tiwari Prakash Nidhi , lama Srijana 
Published: May 2022

AbstractWe present two cases of 10 and 27 years old girls with recurrence of immature teratoma after an incomplete surgical staging. In both cases, there were huge abdominopelvic masses despite decrease in tumor markers with chemotherapy. Complete surgical resection of these masses was done, and histopathology showed only mature teratoma.
Acute polyneuropathy as the main manifestation of primary Sjogren’s syndrome: A case report
Authors: Haj Mohamad Ebrahim Ketabforoush Arsh , Abbasi Khoshsirat Nahid , Maghoul Arman , Nirouei Matineh , Dolatshahi Elahe 
Published: May 2022

AbstractSjogren's syndrome is an inflammatory disease affecting many systems. We report a Sjogren case with the presenting feature of an acute motor‐predominant polyneuropathy resembling Guillain–Barre syndrome. Upon further investigation, it was found that the patient had sicca symptoms for months. Scrupulous history should be taken to prevent a missed diagnosis.
Concomitant coexistence of ACTH‐dependent and independent Cushing syndrome
Authors: Taieb Ach , Ghada Saad , Asma Gorchène , Asma Ben Abdelkrim , Maha Kacem , Koussay Ach 
Published: May 2022

AbstractWe report a case of a patient who presented for ACTH‐dependent Cushing after a confirmed hypercortisolism and an inadequate normal ACTH. A transsphenoidal surgery of a pituitary picoadenoma has been done. After surgery, the patient showed the persistence of hypercortisolism. CT scan revealed adrenal adenomas removed surgically and improved the patient.
Endometrial adenocarcinoma with bone metastasis: A case report
Authors: Mirzaei Monire , Eshraghi Abbas , Ghoddoosi Mahdiieh , Fatemi Maedeh Alsadat , Eshraghi Azhar , Suvvari Tarun Kumar , Fazilat‐Panah Danial 
Published: May 2022

AbstractWe reported a female with endometrial adenocarcinoma who was evaluated with whole‐body bone scan for new‐onset bone pains 6 months after completion of treatment.
Indocyanine green fluorescence: A surgeon’s tool for the surgical approach of gallstone ileus
Authors: Nieto Luis Alejandro , Cabrera‐Vargas Luis Felipe , Lozada‐Martínez Ivan David , Guardo‐Carmona Daniela , Contreras Martin , Pedraza Mauricio , Narvaez‐Rojas Alexis Rafael 
Published: May 2022

AbstractFluorescence cholangiography has been shown to improve biliary anatomy identification. A case of 60‐year‐old man with intestinal obstruction is reported, an entero‐biliary fistula is suspected, and intravenous application of indocyanine green is decided, despite the great inflammatory process and fibrotic tissues found during the procedure, safe open cholecystectomy was achieved.
Salt Solution Concentration Effects on the Electrochemical Impedance Spectroscopy of Poly(3,4‐ethylenedioxythiophene) (PEDOT)
Authors: Sitarik Peter , Martin David C. 
Published: May 2022

AbstractPoly(3,4‐ethylenedioxythiophene) (PEDOT) has become a widely used modifier for biomedical electrodes because of its ability to significantly decrease the impedance at low frequencies (below 1 kHz). However, in past studies the role of the solution concentration (ionic conductivity) on the electrochemical impedance behavior has not been well established. Here we describe the electrochemical impedance spectroscopy of the conjugated polymer (PEDOT) using standard screen‐printed electrodes and various standard salt (NaCl) solutions with known conductivities from 1.0E‐2 S/cm to 3.1E‐6 S/cm. Changing the conductivity of the salt solution used for impedance measurements had a dramatic influence on the experimentally obtained spectra. An equivalent circuit consisting of a constant phase element in series with a parallel resistor and second constant phase element was used to match and describe these systems. Our results make it possible to better elucidate the influence of electrode, solution, and polymer coating on the resulting impedance response.
Late‐Stage Installation of Dehydroamino Acid Motifs into Peptides Enabled by an N‐Chloropeptide Strategy
Authors: Nanjo Takeshi , Oshita Takuma , Matsumoto Ayaka , Takemoto Yoshiji 
Published: May 2022

AbstractConventional methods for the construction of dehydroamino acids (ΔAAs), which are a unique class of non‐proteinogenic amino acids, require the pre‐installation of special amino acids. Herein, we report and demonstrate the practical utility of an N‐chloropeptide strategy for the rapid construction of ΔAA‐containing peptides. The electrophilic N‐chlorination of peptide bonds is drastically accelerated by a catalytic amount of quinuclidine (ABCO), and the subsequent β‐elimination of N‐chloroamide efficiently provides ΔAA‐containing peptides in high yield. The strategy enables, for the first time, the construction of a wide variety of ΔAA residues in peptides without any pre‐functionalized side chains and facilitates the late‐stage installation of ΔAA motifs into already‐constructed oligopeptides, including a medicinally important macrocyclic peptide.
Visible‐Light Photoredox‐Catalyzed Hydrodecarboxylation and Deuterodecarboxylation of Fatty Acids
Authors: Sun Yuan‐Li , Tan Fang‐Fang , Hu Rong‐Gui , Hu Chun‐Hong , Li Yang 
Published: May 2022

Comprehensive SummaryWe develop an efficient visible‐light photoreodox‐catalyzed hydrodecarboxylation and deuterodecarboxylation of fatty acids for alkanes and deuterium alkanes. The key to the efficient transformation should attribute to the co‐catalysis of the suitable methoxy substituted acridine photocatalyst (Mes‐1,3,6,8‐tetramethoxy‐Acr‐3”,5”‐dimethoxy‐Ph) and the hydrogen atom transfer catalyst 4′,4′‐dimethyl diphenyldisulfane, possibly via facilitating of quenching the active alkyl radicals to overcome the competitive homocoupling products, olefins via the disproportionation, cracking products via the C—C bond β‐scission.
Increased population exposure to Amphan‐scale cyclones under future climates
Authors: Mitchell Dann , Hawker Laurence , Savage James , Bingham Rory , Lord Natalie S. , Khan Md Jamal Uddin , Bates Paul , Durand Fabien , Hassan Ahmadul , Huq Saleemul , Islam Akm Saiful , Krien Yann , Neal Jeffrey , Sampson Chris , Smith Andy , Testut Laurent 
Published: May 2022

AbstractSouthern Asia experiences some of the most damaging climate events in the world, with loss of life from some cyclones in the hundreds of thousands. Despite this, research on climate extremes in the region is substantially lacking compared to other parts of the world. To understand the narrative of how an extreme event in the region may change in the future, we consider Super Cyclone Amphan, which made landfall in May 2020, bringing storm surges of 2–4 m to coastlines of India and Bangladesh. Using the latest CMIP6 climate model projections, coupled with storm surge, hydrological, and socio‐economic models, we consider how the population exposure to a storm surge of Amphan's scale changes in the future. We vary future sea level rise and population changes consistent with projections out to 2100, but keep other factors constant. Both India and Bangladesh will be negatively impacted, with India showing >200% increased exposure to extreme storm surge flooding (>3 m) under a high emissions scenario and Bangladesh showing an increase in exposure of >80% for low‐level flooding (>0.1 m). It is only when we follow a low‐emission scenario, consistent with the 2°C Paris Agreement Goal, that we see no real change in Bangladesh's storm surge exposure, mainly due to the population and climate signals cancelling each other out. For India, even with this low‐emission scenario, increases in flood exposure are still substantial (>50%). While here we attribute only the storm surge flooding component of the event to climate change, we highlight that tropical cyclones are multifaceted, and damages are often an integration of physical and social components. We recommend that future climate risk assessments explicitly account for potential compounding factors.
Polygenic Risk Scores for Blood Pressure to Assess the Risk of Severe Bevacizumab‐Induced Hypertension in Cancer Patients (Alliance)
Authors: Quintanilha Julia C.F. , Etheridge Amy S. , Graynor Brady J. , Larson Nicholas B. , Crona Daniel J. , Mitchell Braxton D. , Innocenti Federico 
Published: May 2022

Hypertension is a common bevacizumab‐induced toxicity. No markers are available to predict patients at risk of developing hypertension. We hypothesized that genetic risk of essential hypertension, as measured by a blood pressure polygenic risk score (PRS), would be associated with risk of severe bevacizumab‐induced hypertension. PRSs were calculated for 1,027 bevacizumab‐treated patients of European descent with cancer from four clinical trials (Alliance for Clinical Trials in Oncology (Alliance) / Cancer and Leukemia Group B (CALGB) 80303, 40503, 90401, 40502) using summary systolic blood pressure (SBP) and diastolic blood pressure (DBP) genome‐wide association results obtained from 757,601 individuals of European descent. The association between PRS and grade 3 bevacizumab‐induced hypertension (Common Toxicity Criteria for Adverse Events version 3) in each trial was performed by multivariable logistic regression. Fixed‐effect meta‐analyses odds ratios (ORs) per standard deviation (SD) of the association of PRS (quantitative) and hypertension across trials were estimated by inverse‐variance weighting. PRSs were additionally stratified into quintiles, with the bottom quintile as the referent group. The OR of the association between hypertension and each quintile vs. the referent group was determined by logistic regression. The most significant PRS (quantitative)‐hypertension association included up to 67 single‐nucleotide variants (SNPs) associated with SBP (P = 0.0077, OR per SD = 1.31, 95% confidence interval (CI), 1.07–1.60), and up to 53 SNPs associated with DBP (P = 0.0209, OR per SD = 1.27, 95% CI, 1.04–1.56). Patients in the top quintile had a higher risk of developing bevacizumab‐induced hypertension compared with patients in the bottom quintile using SNPs associated with SBP (P = 4.75 × 10−4, OR = 3.72, 95% CI, 1.84–8.16) and DBP (P = 0.076, OR = 1.83, 95% CI, 0.95–3.64). Genetic variants associated with essential hypertension, mainly SBP, increase the risk of severe bevacizumab‐induced hypertension.
UV‐Induced C−H Functionalization of Alkanes with NO2 in Supercritical Carbon Dioxide
Authors: Ivanov Roman E. , Zharkov Mikhail N. , Zlotin Sergei G. 
Published: May 2022

AbstractA novel catalyst‐free method for functionalization of unbranched alkanes via radical nitration with UV‐activated nitrogen oxide (IV) in supercritical carbon dioxide medium has been developed. The method allows to transform these inactive substrates into corresponding nitrates and nitro compounds with outstanding yields (up to 65 %), using available reagents (NO2, O2) under mild reaction conditions (35–50 °C), and exhibits lower hazards compared to conventional analogues. An improved selectivity towards the nitrates has been achieved by adding pure oxygen to the reaction. Based on changes in reaction rate and product composition, the plausible reaction mechanism for the UV‐induced C−H functionalization of alkanes with nitrogen oxide (IV) and pure oxygen has been proposed.
Visualizing Cell Motility in Mouse Ear Explants
Authors: Petrie Ryan J. 
Published: May 2022

AbstractA method to visualize cell motility in fluorescence‐labeled mouse‐ear dermal explants is described. This approach allows cell and matrix dynamics to be visualized in physiologically relevant, three‐dimensional (3D) environments. This Basic Protocol for the preparation of mouse‐ear dermal explants can be optimized and applied to any tissue explant and cell type. © 2022 Wiley Periodicals LLC.
Pseudocowpox virus, a novel vector to enhance the therapeutic efficacy of antitumor vaccination
Authors: Ramos Rodrigo Nalio , Tosch Caroline , Kotsias Fiorella , Claudepierre Marie‐Christine , Schmitt Doris , Remy‐Ziller Christelle , Hoffmann Chantal , Ricordel Marine , Nourtier Virginie , Farine Isabelle , Laruelle Laurence , Hortelano Julie , Spring‐Giusti Clementine , Sedlik Christine , Le Tourneau Christophe , Hoffmann Caroline , Silvestre Nathalie , Erbs Philippe , Bendjama Kaidre , Thioudellet Christine , Quemeneur Eric , Piaggio Eliane , Rittner Karola 
Published: May 2022

AbstractObjectiveAntitumor viral vaccines, and more particularly poxviral vaccines, represent an active field for clinical development and translational research. To improve the efficacy and treatment outcome, new viral vectors are sought, with emphasis on their abilities to stimulate innate immunity, to display tumor antigens and to induce a specific T‐cell response.MethodsWe screened for a new poxviral backbone with improved innate and adaptive immune stimulation using IFN‐α secretion levels in infected PBMC cultures as selection criteria. Assessment of virus effectiveness was made in vitro and in vivo.ResultsThe bovine pseudocowpox virus (PCPV) stood out among several poxviruses for its ability to induce significant secretion of IFN‐α. PCPV produced efficient activation of human monocytes and dendritic cells, degranulation of NK cells and reversed MDSC‐induced T‐cell suppression, without being offensive to activated T cells. A PCPV‐based vaccine, encoding the HPV16 E7 protein (PCPV‐E7), stimulated strong antigen‐specific T‐cell responses in TC1 tumor‐bearing mice. Complete regression of tumors was obtained in a CD8+ T‐cell‐dependent manner after intratumoral injection of PCPV‐E7, followed by intravenous injection of the cancer vaccine MVA‐E7. PCPV also proved active when injected repeatedly intratumorally in MC38 tumor‐bearing mice, generating tumor‐specific T‐cell responses without encoding a specific MC38 antigen. From a translational perspective, we demonstrated that PCPV‐E7 effectively stimulated IFN‐γ production by T cells from tumor‐draining lymph nodes of HPV+‐infected cancer patients.ConclusionWe propose PCPV as a viral vector suitable for vaccination in the field of personalised cancer vaccines, in particular for heterologous prime‐boost regimens.