bluuebunny/crossref_metadata_2025_split huggingface dataset:
KAJIAN PEMANFAATAN LIMBAH KACA SEBAGAI PENGGANTI AGREGAT HALUS DAN FLY ASH 30% DARI BERAT SEMEN DITINJAU DARI KUAT TARIK BELAH, DAYA SERAP DAN POROSITAS BETON
Authors: Taufiq Lilo Adi Sucipto Cahyo Didit Prasetyo, Ernawati Sri Sunarsih, 
Published: March 2022

Perkembangan IPTEK di Indonesia semakin maju, terutama di bidang bangunan, hal ini memunculkan ide untuk menciptakan bahan bangunan yang relatif murah dan tidak merusak keseimbangan alam. Tujuan dari penelitian ini yaitu untuk mengetahui, 1) pengaruh limbah kaca sebagai bahan pengganti agregat halus terhadap kuat tarik belah beton 2) pengaruh limbah kaca sebagai bahan pengganti agregat halus terhadap daya serap air beton 3) pengaruh limbah kaca sebagai bahan pengganti agregat halus terhadap porositas beton 4) persentase kuat tarik belah makisimal yang dihasilkan dari penggantian agregat halus dengan kaca 5) persentase daya serap air minimal yang dihasilkan dari penggantian agregat halus dengan kaca 6) persentase porositas minimal yang dihasilkan dari penggantian agregat halus dengan kaca. Penelitian ini menggunakan metode eksperimen di laboratorium dengan pengujian sampel. Adapun pengujian yang dilakukan berupa pengujian kuat tarik belah, daya serap dan porositas beton. Berdasarkan hasil penelitian disimpulkan bahwa, variasi penggantian serbuk kaca sebagai agregat halus berpengaruh negatif sebesar 46,2% terhadap kuat tarik belah. Variasi penggantian serbuk kaca sebagai agregat halus berpengaruh negatif sebesar 63,9% terhadap daya serap air beton. Variasi penggantian serbuk kaca sebagai agregat halus berpengaruh negatif sebesar 73% terhadap porositas beton. Persentase serbuk kaca optimal sebagai pengganti agregat halus yang menghasilkan kuat tarik belah beton maksimal terdapat pada persentase penggantian serbuk kaca 10% yaitu sebesar 2,258 MPa, Persentase serbuk kaca optimal sebagai pengganti agregat halus yang menghasilkan daya serap beton minimal terdapat pada persentase penggantian serbuk kaca 10% yaitu sebesar 6,407%, Persentase serbuk kaca optimal sebagai pengganti agregat halus yang menghasilkan porositas beton minimal terdapat pada persentase penggantian serbuk kaca 10% yaitu sebesar 14,006%.
PENGEMBANGAN MEDIA PEMBELAJARAN BERBASIS ALAT PERAGA MINIATUR RUMAH BERTINGKAT SISTEM BONGKAR PASANG PADA MATA KULIAH KONSTRUKSI BANGUNAN GEDUNG III
Authors: Sukatiman Agung Nugroho, A. G. Tamrin, 
Published: March 2022

Tujuan pengembangan media pembelajaran berbasis alat peraga miniatur rumah bertingkat mata kuliah Konstruksi Bangunan Gedung III pada materi potongan denah rumah tinggal adalah (1) Mengembangkan alat peraga miniatur rumah bertingkat sistem bongkar pasang sebagai sarana pembelajaran Mata Kuliah Konstruksi Bangunan Gedung III menjadi lebih baik. (2) Mengetahui tingkat kelayakan alat peraga miniatur rumah bertingkat sistem bongkar pasang Mata Kuliah Konstruksi Bangunan Gedung III pada materi potongan denah rumah tinggal. Penelitian ini menggunakan metode <em>research and development</em> dengan melakukan pengembangkan pada media pembelajaran alat peraga. Adapun tahap pengembangan pada media pembelajaran ini (1) Tahap Studi Pendahuluan: Studi literatur dan studi lapangan. (2) Tahap pengembangan media: penyusunan desain model alat peraga, validasi tim ahli, uji kelayakan pada kelompok kecil dan kelompok besar. (3) Tahap evaluasi merupakan tahap akhir dari proses pengembangan terkait model <em>final</em> media pembelajaran alat peraga. Hasil penelitian sebagai berikut, (1) Pengembangan alat peraga sebagai media pembelajaran berupa penggabungan alat peraga nyata dan modul menjadi satu kesatuan kerja. Satu kesatuan yang dapat menghasilkan suatu informasi dengan materi yang telah disesuaikan dengan RPS Mata Kuliah Konstruksi Bangunan Gedung III dengan materi merencanakan potongan denah rumah tinggal bertingkat. (2) Uji kelayakan media dari ahli materi sebesar 93,06%, termasuk sangat layak. Uji kelayakan media dari ahli media sebesar 89,55% termasuk sangat layak. Uji kelayakan media dari ahli pembelajaran sebesar 89,41% termasuk sangat layak. Uji kelayakan media terhadap mahasiswa skala kecil sebesar 83,4% termasuk sangat layak. Uji kelayakan media terhadap mahasiswa skala besar sebesar 84,38% termasuk sangat layak.
Musculoskeletal Educational Resources for the Aspiring Orthopaedic Surgeon
Authors: Wadhwa Harsh , Van Rysselberghe Noelle L , Campbell Sean T , Bishop Julius A 
Published: March 2022

Abstract
Musculoskeletal (MSK) education is underemphasized in medical school curricula, which can lead to decreased confidence in treating MSK conditions and suboptimal performance on orthopaedic surgery elective rotations or subinternships. Given the low amount of formalized education in MSK medicine, students aiming to learn about orthopaedic surgery must gain much of their foundational knowledge from other resources. However, there are currently no centralized introductory educational resources to fill this need. We provide a framework for navigating the different types of resources available for trainees and highlight the unaddressed needs in this area.
Racial/Ethnic Disparities in Preeclampsia, Eclampsia, and HELLP Syndrome Hospitalizations Rates in the United States
Authors: Dongarwar Deepa , Adindu Ebubechi , Mizu Ruth , Salihu Hamisu 
Published: March 2022

There is a lack of recent data demonstrating how racial/ethnic disparities in the occurrence of preeclampsia, eclampsia, and hemolysis, elevated liver enzymes, low platelet count (HELLP) syndrome may translate into hospitalization rates. Therefore, the purpose of this study was to evaluate the disparities in preeclampsia, eclampsia, and HELLP syndrome hospitalizations rates across racial/ethnic patient populations in the United States (US). The Nationwide Inpatient Sample (NIS) datasets 2016-2018 were used for this analysis. Prevalence rates were calculated for each hypertensive disorder of pregnancy – preeclampsia, HELLP syndrome and eclampsia, by race/ethnicity. Survey logistic regression model was utilized to find the association between race/ethnicity and each phenotype of hypertensive disorder of pregnancy. During the study period, there were 499.4, 27.0 and 12.1 per 10,000 hospitalizations with preeclampsia, HELLP syndrome and eclampsia. The hospitalization rates and odds of a diagnosis of preeclampsia and eclampsia were higher in Non-Hispanic (NH)-Black pregnant population when compared with NH-White pregnant population. The hospitalization rate and odds of having a diagnosis of HELLP syndrome were highest in NH-Whites as compared to other racial/ethnic groups.

Copyright © 2022 Dongarwar et al. Published by Global Health and Education Projects, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution License CC BY 4.0.
Site selection and Storage System Design for RWH with artificial recharge based on hydro-geological and socio-economic consideration in qualitative and quantitative water-stressed areas of North 24 Parganas, India
Authors: Biswas Satabdi , Sahoo Satiprasad , Debsarkar Anupam , Pal Manoranjan , Ray Shyama Prasad Sinha , Al-Quraishi Ayad M. Fadhil 
Published: March 2022

Abstract
Rural households of North 24 Parganasare adversely affected by combined qualitative and quantitative water stress. An attempt is made to establish a relationship between RWH potential based on hydro-geological and socio-economic characteristics. Total 924 rural household data were collected through a rigorous socio-economic survey in four study blocks viz. Barasat II, Amdanga, Barrackpore II, and Basirhat I to assess the water insufficiency. Land-use and Land-cover study showed a rapid increase in the built-up area to the extent of 21.69%, 10.44%, and 4.08% for Barasat II, Amdanga, and Basirhat I blocks, respectively during (2010–2020). Over this period, water bodies were reduced by nearly 19% and 6% in Barasat II and Basirhat I, respectively. The upper aquifer within 40 to 60 m depth was suitable for artificial recharge from the fence diagram. Artificial recharge structures like percolation tank with recharge shaft were suggested (within 40 m depth) for Amdanga block, re-excavation (up to a depth of 3 mbgl) for Basirhat I block and recharge well or pit to be excavated up to(30, 10, and 10 to 20 m) for highly built-up areas of Amdanga, Barasat II and Barrckpore II, respectively. Water quality parameters viz. TDS, ammonia-N, and chloride were found reasonably safe for drinking purposes in the rain water samples. The storage tank's capacity was calculated as 6,000 litres for individual rural households, with 60 gm of bleaching powder estimated for a full tank. The suggested methods would help local authorities successfully execute the RWH schemes inwater-stressed areas.
Cell chirality reversal through tilted balance between polymerization of radial fibers and clockwise-swirling of transverse arc
Authors: Kwong Hoi Kwan , Liu Wengang , Wu Siying , Chung Cho Fan , Chu Lok Ting , Chow Hiu Lam , Hartanto Hogi , Wang Jiachen , Lam Miu Ling , Chen Ting-Hsuan 
Published: March 2022

Abstract

Cell chirality is an intrinsic property shown as biased cell rotation or orientation. While the righthanded helix of actin is known essential, it is intriguing of how single form of molecular handedness can be manifested into diverse and even mirrored forms of cell chirality. Here, we found that cell nucleus rotates with clockwise (CW) bias with smaller projected area but reverses to anticlockwise (ACW) bias when cell spreading increases. Actin analysis suggests that polymerization of righthanded radial fibers accounts for the ACW bias, while the CW bias is driven by the retrograde flow of CW-swirling transverse arc, which originates from tethered myosin II that simultaneously connect radial fibers and transverse arc. Thus, by tilting the balance between these two classes of actin fibers via cell spreading area or the main factors of actin, i.e., myosin II, α-actinin-1, Tpm4, and mDia2, the single form of actin helix can be unfolded into cell chirality with opposite bias. This finding suggests a mechanistic insight of cell chirality reversal, providing new perspective in mechanobiology and tissue formation.
Downregulation of DNA methyltransferase-3a ameliorates the osteogenic differentiation ability of adipose-derived stem cells in diabetic osteoporosis
Authors: Zhang Maorui , Gao Yujin , Li Qing , Cao Huayue , Yang Jianghua , Cai Xiaoxiao , Xiao Jingang 
Published: March 2022

Abstract
Background
Diabetes-related osteoporosis (DOP) is a chronic disease caused by the high glucose environment that induces a metabolic disorder of osteocytes and osteoblast-associated mesenchymal stem cells. The processes of bone defect repair and regeneration become extremely difficult with DOP. Adipose-derived stem cells (ASCs), as seed cells in bone tissue engineering technology, provide a promising therapeutic approach for bone regeneration in DOP patients. The osteogenic ability of ASCs is lower in a DOP model than that of control ASCs. DNA methylation, as a mechanism of epigenetic regulation, may be involved in DNA methylation of various genes, thereby participating in biological behaviors of various cells. The purpose of this study was to investigate the influence of the diabetic environment on the osteogenic potential of ASCs and to improve the osteogenic differentiation ability of ASCs with DOP.
Methods
DOP-ASCs and control ASCs were isolated from DOP C57BL/6 and control mice, respectively. The multipotency of DOP-ASCs was confirmed by Alizarin Red-S, Oil Red-O, and Alcian blue staining. Real-time polymerase chain reaction(RT-PCR), immunofluorescence, and western blotting were used to analyze changes in markers of osteogenic differentiation, DNA methylation, and Wnt signaling. Alizarin Red-S staining was also used to confirm changes in the osteogenic ability. DNMT small interfering RNA (siRNA), shRNA-Dnmt3a, and LVRNA-Dnmt3a were used to assess the role of Dnmt3a in osteogenic differentiation of control ASCs and DOP-ASCs. Micro-computed tomography, hematoxylin and eosin staining, and Masson staining were used to analyze changes in the osteogenic capability when a lentivirus downregulated Dnmt3a in DOP mice in vivo.
Results
The proliferative ability of DOP-ASCs was lower than that of control ASCs. DOP-ASCs showed a decrease in osteogenic differentiation capacity, lower Wnt signaling pathway activity, and a higher level of DNA methylation than control ASCs. When Dnmt3a was downregulated by siRNA and shRNA, osteogenic-related factors Runt-related transcription factor 2 and osteopontin, and activity of the Wnt signaling pathway were increased, which rescued the poor osteogenic potential of DOP-ASCs. When Dnmt3a was upregulated by LVRNA-Dnmt3a, the osteogenic ability was inhibited. The same results were obtained in vivo.
Conclusions
Dnmt3a silencing rescues the negative effects of DOP on ASCs and provides a possible approach for bone tissue regeneration in patients with diabetic osteoporosis.
A data driven method for prioritizing invasive species to aid policy and management
Authors: Finley Dylan , Dovciak Martin , Dean Jennifer 
Published: March 2022

Abstract
Natural resource managers overseeing large regions are often challenged by an overwhelmingly long list of invasive species to prioritize for management and surveys. Often, managers determine priorities through subjective experience and not regional data, contributing to a lack of objectivity, consistency, and transparency. Using the invasion curve as a guiding principle, we developed a data-driven process to guide expert input in creating regionally specific invasive species lists based on management priorities. The invasive species tiers framework uses a standardized set of definitions, data from locational databases and impact assessments, and expert review to categorize high-impact invasive species present in and surrounding the target regions. The analysis process was evaluated and improved by feedback from the structured network of invasive species managers in New York State. Changes between the first and second version of the data tiers analysis increased total number of species represented in the tier lists, accounting for 40% of the differences between the versions. Results of the invasive species tiers process for eight management regions and at the state-scale were made publicly available, and demonstrated variation in invasive species diversity across the management landscape. The approach developed here can be replicated in and scaled to other regions of the U.S. or other countries with comparable data, and it can provide a common management language to better coordinate invasive species management efforts.
Transcriptome analysis of different developmental stages of Daphnia similoides sinensis and screening of male sex determination candidate genes
Authors: Wang Ziyan , Zhang Feiyun , Jin Qide , Wang Yeping , Deng Daogui 
Published: March 2022

Abstract
Background: In the life history of Daphnia, the reproductive mode of parthenogenesis and sexual reproduction alternate in aquatic ecosystem, which are often affected by environmental and genetic factor. Although several functional genes on reproductive transition of Daphnia had been determined, molecular mechanism on the reproductive mode of Daphnia are still not known well, including differentially expressed genes in different developmental stages.Results: In this study, four developmental stages, juvenile female (JF), parthenogenetic female (PF), sexual female (SF) and male (M), of D. similoides sinensis were performed for transcriptome sequence, and candidate genes related to male sex determination were screened. A total of 110437 transcripts were obtained and assembled into 22996 unigenes. In the four developmental stages (JF, PF, SF and M), the number of unique unigenes is respectively 2863, 445, 437 and 586, and the number of common unigenes is 9708. The differentially expressed genes (DEGs) between male and other three female stages (M vs JF, M vs PF and M vs SF) were obtained. GO gene enrichment analysis showed that the up-regulated genes in male were mainly enriched in hydrolase activity and peptidase activity.Thirty-six candidate genes related to male sex determination in male were significantly higher expression than those in the other three stages, including one Doublesex (Dsx) gene, one laminin gene, five trypsin genes and one serine protease genes, and one chitin synthase gene and two chitinase genes. In addition, in D. similoides sinensis male, the relative expression levels of two genes (Dsx1, antp) related to male sex determination observed in other Daphnia species were also significantly higher than those in the other three stages. Conclusions: Our results showed that thirty-six candidate genes may involve in sex differentiation of D. similoides sinensis male, and it will provide a reference for further exploring the functional genes related to sex determination mechanism in Daphnia species. Moreover, according to previous investigations, we thought that the expression level of functional genes may be related to the development stages of organisms, and may be also affected by different Daphnia species.
The GALNS p.P77R variant is a probable Gujarati-Indian founder mutation causing Mucopolysaccharidosis IVA syndrome
Authors: Sheth Harsh , Naik Premal , Shah Maulin , Bhavsar Riddhi , Nair Aadhira , Sheth Frenny , Sheth Jayesh 
Published: March 2022

Abstract
Background: Mucopolysaccharidosis IVA (Morquio syndrome A, MPS IVA) is an autosomal recessive lysosomal storage disorder caused due to biallelic variants in the N-acetylgalactoseamine-6-sulfatase (GALNS) gene. The mutation spectrum in this condition is determined amongst sub-populations belonging to the north, south and east India geography, however, sub-populations of west Indian origin, especially Gujarati-Indians, are yet to be studied. We aimed to analyse the variants present in the GLANS gene amongst the population of Gujarat by sequencing all exons and exon-intron boundaries of the GALNS gene in patients from 23 unrelated families. Results: We report 11 variants that include eight missense variants: (p.L36R, p.D39G, p.P77R, p.C79R, pP125L, p.P151L, p.G255A and p.L350P), one splice site variant: (c.121-7C>G), one small insertion: (c.1241_1242insA, p.I416HfsTer2) and one small deletion: (c.839_41delACA). Of these, three missense variants (p.D39G, p.G255A and p.L350P), one splice site and the two indels mentioned above are novel. Interestingly, we observed a higher than anticipated prevalence of p.P77R variant in our cohort (n=14/25, 56%). Haplotype analysis in cases with p.P77R variant and 63 ethnicity matched healthy population controls suggested a 4 SNP haplotype block present in cases compared to controls (likelihood ratio test p-value= 1.16 x 10-13), thereby suggesting p.P77R variant as a founder variant in the Gujarati Indian population. Furthermore, age of mutation analysis suggested the variant to have arisen approximately 450 years ago in the population. Conclusion: p.P77R variant in the GLANS gene is likely to be a founder variant in MPS IVA patients of Gujarati-Indian ancestry and appeared approximately 450 years ago in the population. To our knowledge, this the first variant to be posited as a founder variant in the GLANS gene in patients with MPS IVA syndrome.
Prognostic analysis of Pneumocystis jirovecii pneumonia in interstitial lung disease patients: a retrospective clinical study
Authors: Sun Yuxin , Shao Chi , Huang Hui , Chen Ruxuan , Xu Kai , Li Mei , Zhang Xin , Xu Zuojun 
Published: March 2022

Abstract
Background
Pre-exiting chronic lung disease was a poor prognostic factor for pneumocystis jiroveci pneumonia (PJP). However, the clinical characteristics and the prognostic factors of HIV negative PJP patients (non-HIV-PJP) with interstitial lung disease (ILD) remains unclear.
Methods
This was a retrospective study of hospitalized patients with confirmed PJP and ILD at Peking Union Medical College Hospital between January 2014 and December 2020. The enrolled patients were stratified based on the presence or absence of ILD and the presence of fibrotic ILD (FILD) or nonfibrotic ILD (non-FILD). The log-rank test and Cox regression models were used to analyze the prognostic factors for PJP patients with different pre-existing statuses.
Results
Among 836 hospitalized individuals with PJP, there were 378 non-HIV-PJP with complete clinical-radiological-follow-up data. Among them, there were 133cases (35.2%) with non-HIV-ILD-PJP, and 70 patients were classified as fibrotic ILD-PJP. The all-cause mortality rate for ILD-PJP group was higher than that of non-ILD-PJP group (57.9% vs 38.4%, Log-rank = 12.2, p < 0.001, However, the all-cause mortality was similar between FILD-PJP and non-FILD-PJP (Log-rank = 0.03, p > 0.05). Co-infection with aspergillosis(HR = 2.89, χ2 = 5.0, p = 0.02, 95%CI:1.14–7.28), honeycomb in the chest HRCT(HR = 16.3, χ2 = 16.8, p < 0.001, 95%CI:4.3–62), invasive ventilation(HR = 3.02, χ2 = 7.1, p < 0.01, 95%CI:1.34–6.82) and non- invasive ventilation(HR = 6.2, χ2 = 16.7, p < 0.001, 95%CI:2.58–14.9 ) were independent survival risk factors for non-HIV-ILD-PJP. Non-invasive ventilation support (HR = 928.56, χ2 = 7.3, p < 0.01, 95%CI:6.6-130796.5) and the serum D-dimer (HR = 1.2, χ2 = 10.9, p < 0.001, 95%CI:1.01–1.34) were independent survival risk factors for fibrotic ILD-PJP patients.
Conclusions
Pre-exiting ILD is independently associated with poor survival of PJP patients. Co-infection with aspergillosis, honeycomb in the chest HRCT, invasive ventilation and non- invasive ventilation were independent survival risk factors for ILD patients complicated with PJP.
Mitral valvuloplasty with the interatrial groove-left atrial dome approach in a patient with Marfan syndrome
Authors: Chen Fengjie , Wang Xiang , Zhuang Xianmian , Guo Hongwei 
Published: March 2022

Abstract
Background
The choice of mitral valve surgical approach has always been a difficult problem in patients with small left atrium.
Case presentation:
We report a case of a patient with Marfan syndrome who underwent the David operation and mitral valvuloplasty. The patient had a small left atrium, so we severed the superior vena cava and opened the interatrial groove and left atrial dome. This method allows for excellent exposure of the mitral valve and subvalvular apparatus, enabling a successful operation.
Conclusion
The interatrial groove-left atrial dome approach provides an option for patients with a small left atrium undergoing mitral valve surgery.
Optimization of Culture Conditions for L-Glutamic Acid production by Bacillus flexus Using Response Surface Methodology
Authors: Adegoke Olaposi O , Kareem Sarafadeen O , Balogun Saka A , Adeogun Abideen I 
Published: March 2022

Abstract
The body can use L-glutamic acid to make proteins (L-GA). It was formerly derived from plants, however bacteria have subsequently been studied as a potential source of synthesis. Bacillus flexus is a glutamic acid-harvesting bacteria that is well-known. Submerged fermentation was used in the production of L-GA. Response Surface Methodology (RSM) was utilized in this study to statistically optimize fermentation parameters that influence Bacillus flexus yield of L-glutamic acid. The effects of culture conditions, including several medium composition components, were evaluated using Central composite design (CCD) response surface optimization. Inoculum size, pH, substrate concentration, temperature, and time were all found to have substantial effects on glutamic acid production. Production increased considerably (p<0.05). Bacillus flexus produced the highest L-glutamic acid production of 4.9 g/L when the substrate concentration was 6%, the inoculum size was 4%, the temperature was 35°C, the pH was 7, and the time was 3 days, as predicted by RSM. This value is very close to the predicted value of 5.02 g/L, proving its validity. RSM proved to be a powerful strategy for improving this organism's L-glutamic acid synthesis.