bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of modern immunizing agent against porcine circovirus type 2 infection based on chimeric VP1-PCV2bCap recombinant protein
Authors: Vrablikova Alena , Fojtikova Martina , Fraiberk Martin , Kotoucek Jan , Kulich Pavel , Brezani Veronika , Gebauer Jan , Novobilsky Adam , Maskova Eliska , Zechmeisterova Kristina , Strakova Nicol , Masek Josef , Psikal Ivan , Hezova Renata 
Published: November 2022

Abstract
Porcine circovirus type 2 is the main causative agent of post-weaning multisystemic wasting syndrome, which affects the immune system of swine and causes widespread epidemics in livestock farms resulting in significant piglet mortality and economic losses every year. Although several commercial vaccines were developed, the efficiency and safety need to be improved. Therefore, we have engineered the chimeric complex containing PCV2bCap protein based on virus like particles (VLPs) and the mouse polyomavirus (MPyV) as VLPs represent modern and safe alternative of classical vaccine with high B cells stimulating activity. The ability of this complex to induce an immune response in both mouse and pig models in vivo were evaluated. Firstly, experimental mice were divided into 4 groups and immunized with sterile buffer and VP1-PCV2bCap with different adjuvants, the immune response was monitored for 10 weeks. Robust immune response was detected after the first immunization and gradually increased after the second and third dose, especially in mice immunized by recombinant protein with Emulsigen (10%) as an adjuvant. Subsequently, to confirm the vaccine efficacy in a target organism, 8-week-old piglets were immunized with VP1-PCV2bCap protein with Emulsigen (10%). The levels of anti-PCV2b specific IgG antibodies were significantly increased in piglets after the second immunization. Finally, strong neutralizing activity of these antibodies was confirmed in PK-15 cells infected with PCV2 Stoon 1010. VP1-PCV2bCap protein complex appears as a promising candidate vaccine for preventing disease associated with PCV2 infection in pigs.
Stereo PIV study of laminar-turbulent transition at 180o sharp bend inside a rectangular duct
Authors: Ali Nishab , Tariq Andallib 
Published: November 2022

Abstract
See manuscript PDF file for full abstract with equations.
Evolution of fully developed laminar flow (𝑅𝑒 = 800) around 180° sharp bend of a rectangular duct, and its subsequent transition into turbulent flow has been characterized by using the Stereo Particle Image Velocimetry (stereo-PIV, 2D3C) system. Results have been captured for numerous imaging planes to develop a fundamental understanding of flow progression inside the duct. A shear layer that separates the region of mainstream turning flow from the recirculation region (created near the divider wall) is observed at the recirculation boundary. Centrifugal instabilities in the mainstream turning flow arise by the sharp bend, and thus the secondary flows in the form of a counter-rotating pair of Dean vortices get induced. Two pairs of counter-rotating Dean vortices are being formed after 90° turning (at plane P7y−z), which merge into single pair after 180° turning (at plane P8x−y). It is observed that these set of Dean vortices plays a very significant role in the laminar-turbulent transition at the bend. The complete 180° turn results in a sharp 1825% increment in spatially averaged turbulent kinetic energy (k), and a 287% rise in spatially averaged wall normal root mean square (RMS) velocity fluctuations (vrms), at vertical planes in the direction of flow progression.
Complete chloroplast genomes of three cultivated Cucurbita species: insights into adaptive evolution, phylogenetic relationship, and cpSSR marker development
Authors: Wang Putao , Zhou Cong , Zhang Yu , Wu Caijun , Zeng Qun , Zeng Chenghong , Zhou Qinghong , Zhu Qianglong 
Published: November 2022

Abstract
Background: Cucurbita moschata, C. maxima, and C. argyrosperma are the most well-known horticultural crops of the Cucurbitafamily. These Cucurbita species are taxonomically and phylogenetically puzzling resulted from similar feature and interspecies hybridization.
Results: The complete chloroplast genome size of C. moschata, C. maxima, and C. argyrosperma were assembled and studied for the first time in this study, with genome sizes of 157,644, 157,204, and 157,809bp, respectively. The 130 annotated genes were identified in these genomes, comprising eight rRNA, 37 tRNA, and 85 protein-coding genes, were organized into conserved quadripartite circular topologies. A comparison within Cucurbitashowed that the highly variable region (trnL-ndhJ) was identified to serve a specific DNA barcode, and three genes (atpE, clpP, and ycf1) showed significant selective sites under positive selection. Phylogenetic analysis confirmed that the five cultivated crops of Cucurbita were clustered into one monophyletic group, suggesting that the completion of more chloroplast genomes would help clarify the phylogenetic relationship among Cucurbita. Additionally, ten polymorphic SSRs were identified, which can be used to explore the evolutionary relationships and identify Cucurbita.
Conclusion: These results will help identify the species, molecular breeding, adaptive evolution, and phylogenetic relationshipsof Cucurbita.
Intestinal endogenous metabolites affect neuroinflammation in 5 ×FAD mice by mediating "gut-brain" axis and the intervention with Chinese Medicine
Authors: Gu Xinru , Zhou Yanyan , Zhang Yan , Wang Linna , Gao Wenya , Luo Keke , Sun Bo , Li Tao , Wang Hongjie , Si Nan , Wei Xiaolu , Bian Baolin , Zhao Haiyu 
Published: November 2022

Abstract
Emerging evidence suggested the association between gut
dysbiosis and Alzheimer’s disease (AD) progression. However, it
remains unclear how the gut microbiome and neuroinflammation in the
brain mutually interact or how these interactions affect brain
functioning and cognition. Here we hypothesized that “gut-brain”
axis mediated by microbial derived metabolites was expected to
novel breakthroughs in the fields of AD research and
development.

Methods: Multiple technologies, such as
immunofluorescence, 16s rDNA sequencing, mass spectrometry-based
metabolomics (LC-QQQ-MS and GC-MS), were used to reveal potential
links between gut microbiota and the metabolism and cognition of
the host. Results: Microbial depletion induced by
antibiotic cocktail verified that “gut-brain” can transmit
information bidirectionally. SCFAs-producing bacteria and
amino-producing bacteria fluctuated greatly in 5 ×FAD mice,
especially the reduction sharply of the Bifidobacteriaceae
and the increase of the Lachnospiraceae family.
Concentrations of several Tryptophan-kynurenine intermediates,
lactic acid, CD4+ cell, and CD8+ cells were
higher in serum of 5 ×FAD mice, whilst TCA cycle intermediates and
Th1/Th2 were lower. In addition, the levels of iso-butyric acid
(IBA) in fances, serum, and brain of 5 ×FAD mice were increased
compared with WT-M mice, especially in serum. And IBA in the brain
was positively correlated with Aβ and proinflammatory
factors.

Conclusion Together, our finding highlighted
that the alternation in gut microbiota affected the effective
communication between the “gut-brain” axis in 5 ×FAD mice by
regulating the immune system, carbohydrate, and energy
metabolism.
Hydrological drought severity in different return periods in rivers of Ardabil Province, Iran
Authors: Ghabelnezam Elnaz , Mostafazadeh Raoof , Hazbavi Zeinab 
Published: November 2022

Abstract
Considering the critical role of hydrological drought in water exploitation and management, this study was conducted to analyze the drought severity in different return periods in rivers of Ardabil Province (area = 17,953 km2). To this end, the Streamflow Drought Index (SDI) was computed using DrinC software at 1, 3, and 6-month time scales for 25 hydrometric stations during 1981–2014. Then, the drought intensity was evaluated with CumFreq software in different return periods (2, 5, 10, 25, 50, and 100). Finally, the relationship between discharge, SDI, and return periods was analyzed using triple diagram models. The results revealed the mild and moderate drought intensity for whole study stations of Ardabil Province. The mean values of SDI in 1, 3, and 6-month time scales were 2.37, 1.96, and 1.89, respectively. At all study time scales, the drought intensity increases with increasing return period in rivers with low and high flows, to the extent that occurs in the 100-year return period in rivers with high flow.
Geospatial Database Design for implementing enhanced Local Governance with spatial analysis: A Case study of Manesar area – India
Authors: Dev Manuj , Krishna Vanama Venkata Sai , Kumar Deepak , Kainthola Ashutosh , Jha Manas Kumar 
Published: November 2022

Abstract
Multi-agency investments require local coordination, planning, and implementation. GIS allows us to analyze and comprehend real-world processes by constructing and applying manipulation, analytical criteria, and models for daily decision making. This requires a geospatial database with surveyed land, assets, hydro, etc. Survey of India (SOI) is publishing a topographical map of India with significant cities, areas of interest, and a geodetic benchmark. Different states in India have set up a complete geographic database with land parcels, asset mapping, and administrative borders usually based on the national land record modernization program survey. Many state and local governments also have geospatial databases. Existing geospatial database designs are based on individual requirements that have generated a large volume of duplicate, unformatted, unstructured data. These datasets lacked coordination or logical intercorrelation with other departments' datasets. It served a single department's needs. This causes redundant data, low quality, and a lack of data understanding. The problem's appropriate representation is key to solving it. The Study creates a Common Geo Database for the region to improve collaboration and working. After consulting civil, electrical, and planning engineers, a common database is created. GIS adoption has improved day-to-day decision-making.
Design of a novel instrument for cold excision of bladder tumors under local anesthesia
Authors: Wu Ronghua , Liu Xing , Feng huan , Yajun Song , Ya Xiao , Chen Wei , Yi Shanhong , Fu Weihua 
Published: November 2022

Abstract
Objective: To design a new cold dissection instrument for bladder tumors that can be used under local anesthesia without the need for general or intralesional anesthesia. The instrument will be used for cold dissection of bladder tumors based on the existing cystoscopy routine operation channel. The device will be combined with the cocktail protocol to accomplish cold resection of bladder tumors under local anesthesia
METHODS: 3-dmax mapping was used to design the instrument by combining the existing Endoscopic Submucosal Dissection technique in gastroenterology with the existing bladder injection needle and bipolar electrocoagulation equipment in the clinic. We also assessed the effect of "cocktail" anesthesia on the prevention of closed nerve reflex during tumor resection.
RESULTS: The prototype this device was successfully designed using 3dmax. We then assessed if the injection needle, electrocoagulator, and ligature ring commonly used in clinical practice can be used in combination based on the existing cystoscopic access，The existing instruments used in the department of gastroenterology can complete the functions we imagined, and the physical size can meet the requirements of the existing cystoscope endoscope channel. We also demonstrated that the cocktail protocol was successfully in preventing the occurrence of Obturator reflex of bladder lateral wall tumor.
CONCLUSION: The instrument was successfully developed and used for cold excision of bladder tumors in combination with the cocktail protocol. This instrument has wide clinical utility as its use can be extended to all scenarios requiring endoscopic resection, including gastroscopy.
Selective anatomical catheter ablation of left atrial ganglionated plexus for vasovagal syncope: left superior and right anterior ganglionated plexus ablation
Authors: Lin Jianwei , Liu Qiang , Jiang Ruhong , Chen Shiquan , Yu Lu , Jiang Chenyang 
Published: November 2022

Abstract
Background
Vasovagal syncope (VVS) is one of the most common causes of syncope. Traditional treatment has not achieved satisfactory results. The purpose of this study was to assess the feasibility and efficacy of selective anatomical catheter ablation of left atrial ganglionated plexus (GP) as a therapeutic strategy for the patients of symptomatic VVS.
Methods
A total of 70 patients with at least once recurrent syncopal episodes of VVS with positive head-up tilt test were enrolled. They were divided into GP ablation group and control group. Patients in GP ablation group were received anatomical catheter ablation of left superior ganglionated plexus (LSGP) and right anterior ganglionated plexus (RAGP). Patients in control group were guideline-direted conventional therapy. The primary endpoint was VVS recurrences. The secondary endpoint was recurrence of syncope and prodrome events.
Results
There were no statistical differences in clinical characteristics between the ablation group (n = 35) and control group (n = 35). Over a follow-up of 12 months, ablation group had significantly lower syncope recurrence compared with control group (5.7% vs. 25.7%, P = 0.02), and ablation group had significantly lower syncope and prodrome recurrence compared with control group (11.4% vs. 51.4%, P < 0.001). In GP ablation, 88.6% of the patients showed significant vagal response during LSGP ablation, and 88.6% of the patients showed significant increased heart rate during RAGP ablation.
Conclusions
For patients with recurrent VVS, selective anatomical catheter ablation of LSGP and RAGP is superior to conventional therapy in reducing syncope recurrence.
RNA splicing of a long cox2i691 and the evolution of cox2 introns in angiosperm mitochondria
Authors: Edera Alejandro A. , Williams Anna V. , Howell Katharine A. , Nevill Paul G. , Small Ian , Sanchez-Puerta M. Virginia 
Published: November 2022

Abstract
In angiosperms, the mitochondrial cox2 gene harbors up to two introns, commonly referred to as cox2i373 and cox2i691. Although each of these introns is ~1.5 kb in length, a cox2i691 of 4.2 kb has been reported in Acacia ligulata (Fabaceae). It is still unclear whether its unusual length is due to a trans-splicing arrangement, the loss of functionality of the interrupted cox2, or a rare elongation of a cis-spliced intron. Here, we tried to shed light on these hypotheses by thoroughly analyzing short-read RNA sequencing with a multi-step computational strategy. The results revealed that the Acacia cox2 is very likely functional and its long intron is spliced in cis in a very efficient manner despite its length. To better characterize how conserved cox2i691 is among angiosperms, we studied 222 fully-sequenced mitogenomes from 30 plant orders. Unlike cox2i373, our results point out that cox2i691 shows a distribution among plants that is shaped by frequent intron loss events driven by localized retroprocessing. In addition, cox2i691 undergoes episodic elongations that are taxonomically scattered and poorly related to genome-wide repeat content. Such elongations were found to occur within domain IV and seem to be due to the incorporation of exogenous DNA. Surprisingly, we found that cox2i691 is erroneously annotated as absent in 30 mitogenomes deposited in public databases.
DCNN Based Technique to Analyze the Data of Foggy and Snowy Season
Authors: Harne Sachin , Choubey Siddhartha , Choubey Abha 
Published: November 2022

Abstract
Image Quality Analysis (IQA) is most significant concepts that is gaining attention among the researchers. Most images are considerably influenced by ambient lights. HumanVisual System perceives theimages quality in day and night time that causes the deprivation of luminance and features. In this article, we put forward an adaptive fuzzy-based DCNN technique that estimates the loss and enhancement of the images during seasonal changes. The natural scenery images are collected and preprocessed using Gaussian filter method that removes the unwanted noises. The preprocessed image is then guided with a guided filter method that helps to segment the seasonal changes of an image. The statistical features are extracted from the Adaptive Fuzzy-based Gamma Correction method that specifically leverages the gamma parameters using fuzzy-based decisional approach. Further on, the Deep Convolutional Neural Networks (DCNN) is running to classify the seasonal changes on images based on the computed image quality score. Investigational result have proven the accuracy of suggested technique in concerning of accuracy, precision and recall.
Elevation Determines the Productivity of Large Cardamom (Amomum subulatum Roxb.) Cultivars in Sikkim Himalaya
Authors: Lepcha Patrush , Gaira Kailash S. , Pandey Aseesh , Chettri Santosh Kumar , Lepcha Jarina , Lepcha Jhony , Joshi Rajesh 
Published: November 2022

Abstract
Large cardamom (Amomum subulatum Roxb.) is an economically important cash crop that provides a livelihood option for the rural communities in the Khangchendzonga Landscape (KL), including Sikkim and supports a large value-chain of diverse stakeholders across the globe. This study explores the influence of elevation on yield-related traits of the large cardamom cultivars grown at different elevations in Sikkim. We evaluated twelve morphometric traits, including eight yield-related traits (number of capsules per spike, capsule length, capsule width, fresh capsule weight, dry capsule weight, number of seeds per locule, fresh seed weight, and dry seed weight) in five large cardamom cultivars (Dzongu Golsey, Sawney, Seremna, Ramsey, and Varlangey) from Sikkim, cultivated at 41 different locations with elevation ranging between 975–2069 m asl. The statistical analysis indicated a significant variability (P < 0.05) in morphometric traits including yield-related traits (except capsule length and width) in five large cardamom cultivars. The cultivars cultivated at an elevation below 1500 m, such as Dzongu Golsey, Sawney, and Seremna, had higher values for yield-related traits than those growing at elevations above 1500 m, and the yield-related traits were negatively correlated with the elevation. Likewise, Varlangey and Ramsey, cultivated above 1500 m elevation, performed better than the same cultivars grown below 1500 m of elevation and depicted a positive correlation between elevation and yield-related traits. The study revealed that the elevation determines the performance of the larger cardamom progression, and suggests cultivating the cultivars in their suitable elevation range for better productivity.
Is bottom trawling the most destructive method of fishing? Evidence from New Zealand’s commercial fisheries.
Authors: Flanagan Pat , Genc Murat , Kahui Viktoria 
Published: November 2022

Abstract
In New Zealand, fisheries are managed by the Individual Transferable Quota (ITQ) system which limits the total catch to a biologically sustainable quota and secures individuals a tradable portion. The incentives to quota holders are to use the most efficient fishing methods, of which bottom trawling is widely perceived as the most destructive. Regulations aim to reduce the effects of destructive fishing methods, however, no quantitative studies exist to examine how effective these are. We merge a comprehensive dataset of catch and effort between 2011 and 2021 with a manually assembled dataset on the exploitation status of 115 ITQ fish stocks in New Zealand to estimate the impact of fishing method on sustainability. Our results show that a 10 percentage point increase in the share of midwater trawling has, by far, the biggest impact, leading to a cumulative increase of 17.79% percent in the probability of overexploitation. Against all expectations, bottom trawling has no statistically significant effect, while bottom longlining increases the probability by 5.57%. These results are in line with recent legislative changes in New Zealand that aim to mitigate the impacts of bycatch from midwater trawling and studies like this can be useful in monitoring their effectiveness.
Nonlinear dynamics of boring process considering unstable region of CNTs-reinforced composite cutter
Authors: Zhang Jinfeng , Wang Zhong , Feng Chao , Jia Junlei , Tong Zhenfang , Ren Yongsheng , Zhong Peisi , Cao Xiaolong 
Published: November 2022

Abstract
The nonlinear dynamic analysis of rotating composite boring round bar containing carbon nanotubes (CNTs) in cutting system was investigated. Firstly, simplify the boring bar to the model of non-extendable rotary cantilever structure. Both the Halpin-Tsai model and micro-mechanical layering theory were used to predict the material properties of the boring round bar. Then the equations for the composite boring bar based on Euler Bernoulli shaft theory, including von Karman geometric nonlinearity are derived. The nonlinear dynamic model of cutting system including periodic regenerative chatter cutting force, periodic control force, viscoelastic and process damping is established by using Hamilton principle. The analytical solution of the steady-state response of the cutting system was subsequently obtained by the Galerkin approximation and the perturbation method of multiple time scales. Finally, the influences of carbon nanotube-related parameters, fiber volume fraction, fiber orientations, stacking sequences, damping coefficient and geometry properties of the cutter edge on the stability of the cutting system are evaluated. The obtained results show that the incorporation of CNTs has significant effect on the dynamic behavior of the cutting process. Increasing the process damping and changing the cutter edge and bar's cross section can improve stability of the cutting process. Furthermore, the unstable cutting region is sensitive to multi-valued properties generated by jumping.
Selective HDAC6 inhibition has the potential for anti-cancer effect in renal cell carcinoma
Authors: Anraku Tsutomu , Murata Masaki , Kuroki Hiroo , Kazama Akira , Shirono Yuko , Tasaki Masayuki , Bilim Vladimir , Tomita Yoshihiko 
Published: November 2022

Abstract
Background: Despite advances in systemic therapy for renal cell carcinoma (RCC), most metastatic RCCs remain incurable. Therefore, new therapeutic approaches are needed to further improve the outcome of RCC treatment.
Methods: We treated cultured RCC cells with a panel of 12 small molecule selective HDAC (histone deacetylase) 6 inhibitors or genetically knocked down HDAC6.
Results: HDAC6 expression was confirmed in pathological RCC specimens and cultured RCC cell lines by immunohistochemical staining and western blot respectively. Most of the HDAC6 inhibitors we used decreased cell viability and proliferation in a concentration-dependent manner in the low micromolar range. Furthermore, HDAC6 inhibition induced apoptosis in RCC cells. The major imitation of our study is the lack of in vivo experiments.
Conclusions: HDAC6 inhibitors decreased cell viability and proliferation by inducing apoptosis in RCC cells. Our results suggest that HDAC6 inhibitors are promising anti-cancer agents, which should be validated in future in vivo studies.