bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased NUSAP1 expression is associated with lymph node metastasis and survival prognosis in bladder urothelial carcinoma
Authors: Hou Jian , Lu Zhenquan , Liu Xianhua , Luo Bingfeng , Qu Genyi , Xu Yong , Tang Cheng 
Published: April 2022

AbstractThe main route of metastasis of bladder urothelial carcinoma is through lymph nodes; however, its exact mechanism remains unclear. In this study, we found an association of nucleolar and spindle associated protein 1 (NUSAP1) expression with BUC tissues along with lymph node metastasis and the survival prognosis. A total of 178 pathological specimens following radical bladder cancer resection were obtained. NUSAP1 expression was analyzed by immunohistochemistry. We evaluated the correlation between clinicopathological characteristics and NUSAP1 expression. Logistic regression was used to determine the independent variables that influenced lymph node metastasis. Uni- and multi-factorial Cox regression methods were used to determine the prognostic value of NUSAP1 expression in urothelial carcinoma of the bladder. High expression of NUSAP1 in BUC was not significantly related to the patient’s gender, age, or tumor number (p > 0.05), however was significantly associated with pathological grade, tumor diameter, pathological stage, and lymph node metastasis (p < 0.05). Lymph node metastasis was significantly correlated with pathological stage, pathological grade, tumor number, tumor diameter, and NUSAP1 expression (p < 0.05); only NUSAP1 expression was an independent predictor of lymph node metastasis in BUC (OR:1.786, 95% CI 1.229–2.596, p = 0.002). In addition, high NUSAP1 expression was an independent prognostic predictor for BUC. In BUC, NUSAP1 showed high expression and was significantly associated with lymph node metastasis, pathological stage, pathological grade, and tumor diameter. NUSAP1 was an independent predictor of lymph node metastasis and prognosis in BUC; higher expression indicated poorer prognosis of BUC patients.
Dynamic compensation of stray electric fields in an ion trap using machine learning and adaptive algorithm
Authors: Ghadimi Moji , Zappacosta Alexander , Scarabel Jordan , Shimizu Kenji , Streed Erik W. , Lobino Mirko 
Published: April 2022

AbstractSurface ion traps are among the most promising technologies for scaling up quantum computing machines, but their complicated multi-electrode geometry can make some tasks, including compensation for stray electric fields, challenging both at the level of modeling and of practical implementation. Here we demonstrate the compensation of stray electric fields using a gradient descent algorithm and a machine learning technique, which trained a deep learning network. We show automated dynamical compensation tested against induced electric charging from UV laser light hitting the chip trap surface. The results show improvement in compensation using gradient descent and the machine learner over manual compensation. This improvement is inferred from an increase of the fluorescence rate of 78% and 96% respectively, for a trapped $$^{171}$$

171

Yb$$^+$$

+

ion driven by a laser tuned to $$-7.8$$

-
7.8

MHz of the $$^2$$

2

S$$_{1/2}\leftrightarrow ^2$$

1
/
2

↔
2

P$$_{1/2}$$

1
/
2

Doppler cooling transition at 369.5 nm.
A lyophilized colorimetric RT-LAMP test kit for rapid, low-cost, at-home molecular testing of SARS-CoV-2 and other pathogens
Authors: Song Xin , Coulter Felicity J. , Yang Ming , Smith Jessica L. , Tafesse Fikadu G. , Messer William B. , Reif John H. 
Published: April 2022

AbstractAccess to fast and reliable nucleic acid testing continues to play a key role in controlling the COVID-19 pandemic, especially in the context of increased vaccine break-through risks due to new variants. We report a rapid, low-cost (~ 2 USD), simple-to-use nucleic acid test kit for self-administered at-home testing without lab instrumentation. The entire sample-to-answer workflow takes < 60 min, including noninvasive sample collection, one-step RNA preparation, reverse-transcription loop-mediated isothermal amplification (RT-LAMP) in a thermos, and direct visual inspection of a colorimetric test result. To facilitate long-term storage without cold-chain, a fast one-pot lyophilization protocol was developed to preserve all required biochemical reagents of the colorimetric RT-LAMP test in a single microtube. Notably, the lyophilized RT-LAMP assay demonstrated reduced false positives as well as enhanced tolerance to a wider range of incubation temperatures compared to solution-based RT-LAMP reactions. We validated our RT-LAMP assay using simulated infected samples, and detected a panel of SARS-CoV-2 variants with successful detection of all variants that were available to us at the time. With a simple change of the primer set, our lyophilized RT-LAMP home test can be easily adapted as a low-cost surveillance platform for other pathogens and infectious diseases of global public health importance.
The relationship of muscular endurance and coordination and dexterity with behavioral and neuroelectric indices of attention in preschool children
Authors: Kao Shih-Chun , Tsai Yu-Jung , Hsieh Shu-Shih , Chen I-Fan , Schmitt Sara , Hung Tsung-Min 
Published: April 2022

AbstractThis study investigated the associations of non-aerobic fitness (NAF) and motor competence (MC) with attention in 4–6 year-old preschoolers. The allocation of attentional resources and speed of stimulus categorization were examined using the amplitude and latency of P3 of event-related potentials respectively, while cortical activation related to general attention and task-specific discriminative processes were examined using event-related desynchronization (ERD) at lower (8–10 Hz) and upper (10–12 Hz) alpha frequencies, respectively. Seventy-six preschoolers completed NAF (muscular power, muscular endurance, flexibility, balance) and MC (coordination and dexterity, ball skills, agility and balance) test batteries. Electroencephalogram was recorded while participants performed an auditory oddball task. After controlling for age and MC, muscular endurance was positively related to P3 amplitude. MC and its coordination and dexterity sub-component were positively related to task performance, with higher levels of coordination and dexterity showing an additional association with greater upper alpha ERD between 700 and 1000 ms following stimulus onset after controlling for age and NAF. These findings suggest relationships of NAF and MC with early childhood neurocognitive function. Specifically, muscular endurance is related to the neuroinhibition in facilitating effective allocation of attentional resources to stimulus evaluation while coordination and dexterity are related to cortical activation underlying strategic attentional preparation for subsequent stimulus evaluation.
Parathyroid venous sampling for the preoperative localisation of parathyroid adenoma in patients with primary hyperparathyroidism
Authors: Ho Joon , Kim Donggyu , Lee Ji-Eun , Hong Namki , Kim Byung Moon , Kim Dong Joon , Kim Jinkyong , Lee Cho Rok , Kang Sang-Wook , Jeong Jong Ju , Nam Kee-Hyun , Chung Woong Youn , Rhee Yumie 
Published: April 2022

AbstractPreoperative localisation studies are essential for parathyroidectomy in patients with primary hyperparathyroidism. If the location of abnormal parathyroid glands cannot be identified through non-invasive studies, parathyroid venous sampling (PVS) may be employed. In this study, we evaluated the utility of preoperative PVS in parathyroid surgery. Patients with primary hyperparathyroidism who underwent preoperative PVS at Severance Hospital between January 2015 and June 2020 were identified. Patients for whom the results of non-invasive imaging studies were inconsistent or negative underwent PVS. The results of PVS were compared with operative findings and pathologic results. For 14 patients, the results of preoperative ultrasonography and 99mTc-sestamibi single-photon emission computed tomography (SPECT) were negative; for 20 patients, either the result of only one test was positive, or the results of the two tests were inconsistent. With respect to the lateralisation of diseased adenoma, the results of PVS and pathological examination were inconsistent only for one patient in either group (total: 2/34 patients). This study showed that PVS could be used effectively for preoperative localisation in patients with primary hyperparathyroidism in whom the location of diseased parathyroid glands cannot be determined through non-invasive image studies.
Scaffold stability and P14′ residue steric hindrance in the differential inhibition of FXIIa by Aedes aegypti trypsin inhibitor versus Infestin-4
Authors: Walvekar Varsha Ashok , Ramesh Karthik , Kannan Muthu , Kini R. Manjunatha , Sivaraman J. , Mok Yu Keung 
Published: April 2022

Abstract
Kazal-type protease inhibitors strictly regulate Factor XIIa (FXIIa), a blood-clotting serine protease. However, when negatively charged surface of prosthetic device come into contact with FXII, it undergoes conformational change and auto-activation, leading to thrombus formation. Some research suggests that Kazal-type protease inhibitor specificity against FXIIa is governed solely by the reactive-site loop sequence, as this sequence makes most-if not all-of the direct contacts with FXIIa. Here, we sought to compare the inhibitory properties of two Kazal-type inhibitors, Infestin-4 (Inf4), a potent inhibitor of FXIIa, and Aedes aegypti trypsin inhibitor (AaTI), which does not inhibit FXIIa, to better understand Kazal-type protease specificity and determine the structural components responsible for inhibition. There are only three residue differences in the reactive-site loop between AaTI and Inf4. Through site-directed mutagenesis, we show that the reactive-site loop is only partially responsible for the inhibitory specificity of these proteases. The protein scaffold of AaTI is unstable due to an elongated C5C6 region. Through chimeric study, we show that swapping the protease-binding loop and the C5C6 region from Inf4 with that of AaTI can partially enhance the inhibitory activity of the AaTI_Inf4 chimera. Furthermore, the additional substitution of Asn at the P14′ position of AaTI with Gly (Gly27 in Inf4) absolves the steric clashing between AaTI and the surface 140-loop of FXIIa, and increases the inhibition of the chimeric AaTI to match that of wild-type Inf4. Our findings suggest that ancillary regions in addition to the reactive-site loop sequence are important factors driving Kazal-type inhibitor specificity.
Prevalence of Self-Reported Hearing Loss and Associated Risk Factors: Findings From the Survey of the Health of Wisconsin
Authors: Dillard Lauren K. , Walsh Matthew C. , Merten Natascha , Cruickshanks Karen J. , Schultz Amy 
Published: April 2022

Purpose:
The purpose of this study was to determine the prevalence of self-reported hearing loss and associated risk factors in a representative population-based study of Wisconsin residents.

Method:
Survey of the Health of Wisconsin participants with data on self-reported hearing loss were included. We reported prevalence of self-reported hearing loss with corresponding 95% confidence intervals (CIs), overall, and stratified by age and sex. Age- and sex-adjusted and multivariable logistic regression models were used to evaluate determinants of self-reported hearing loss, and results are presented as odds ratios with corresponding 95% CIs.

Results:
There were 2,767 participants (50.7% men) with a mean age of 46 years (range: 21–74) in this study. Prevalence of self-reported hearing loss was 26.8% (24.4, 28.4) and was higher in men (30.3% [27.1, 33.4]) than in women (22.5% [19.9, 25.0]). Prevalence increased with age. After multivariable adjustment, age (per +1 year increase; 1.05 [1.04, 1.06]), male sex (1.57 [1.18, 2.08]), having two chronic diseases (vs. 0; 1.93 [1.16, 3.23]), occupational (2.47 [1.91, 3.19]) and recreational (1.58 [1.22, 2.04]) noise exposure, and poor diet (1.88 [1.28, 2.78]) were associated with higher odds of self-reported hearing loss.

Conclusions:
Hearing loss is a highly prevalent public health concern and may be at least partially modifiable via interventions to reduce noise exposure and promote health. Statewide prevalence and risk factor data can be used to inform public health practice and promote hearing loss prevention.

Supplemental Material:

https://doi.org/10.23641/asha.19661130
Few‐sample multi‐objective optimisation of a double‐sided tubular machine with hybrid segmented permanent magnet
Authors: Guo Liang , Weng Mian , Galea Michael , Wu Xiaowen , Zhang Peng , Lu Wenqi 
Published: April 2022

AbstractDouble‐sided tubular machine (DSTM) is very suitable for wave energy conversion but easily suffers from high thrust ripple. In order to get the minimum cogging force with the maximum thrust force, a new DSTM with hybrid segmented permanent magnet array is proposed and optimised by a novel iterative few‐sample multi‐objective optimisation method. The novel optimisation method is based on an iterative Taguchi method framework to obtain optimal design with only few samples. To solve the low precision problem of the iterative Taguchi method, a surrogate‐model based multi‐objective optimisation algorithm that uses a general regression neural network, a speed‐constrained multi‐objective particle swarm optimisation and an exponentially weighted moving average are embedded into this framework. The optimisation result is compared with other alternative topologies and methods, and a prototype is manufactured for testing experiment.
Risk transfer assessment model of integrated energy system in the process of offshore oil and gas production
Authors: Zhang Anan , Zhao Zhihui , Peng Gaoqiang , Zaman Roksana , Li Qian 
Published: April 2022

AbstractThe offshore integrated energy system plays important role in the offshore oil and gas processing industry. To ensure the safe development of offshore oil and gas resources, accurately assessing their risks is crucial. First, this article describes the risk factors that affect the operation of the equipment and builds a risk model that takes into account the relevance of the coupled system; then, a risk transfer model based on material–energy analysis is proposed, which describes the transfer of risk through material–energy flow. In addition, according to the structure and operating characteristics of the offshore integrated energy system, a risk indicator system has been designed from five levels: operational risk, energy supply risk, structural risk, economic risk and environmental risk. Finally, by taking the simulation experiment of an offshore oil and gas platform, the proposed model in this paper evaluates the risks of offshore oil and gas platforms accurately.
Influence of surface discharge on the deterioration characteristics of ethylene‐propylene rubber cable insulation under alternating current high voltage
Authors: Bai Longlei , Fan Daoping , Li Tianhe , Li Bing , Su Mingwei , Fan Shaogui , Zhang Liuzhong 
Published: April 2022

AbstractTo investigate partial discharge‐induced deterioration characteristics of ethylene‐propylene rubber (EPR) cable insulation under repeated alternating current (AC) voltage on high‐speed trains, an experimental platform with a column‐plate EPR insulation model is established. The surface condition and breakdown voltage of insulation samples after discharge treatment are observed and measured. In addition, a fitting formula to evaluate the deterioration extent of EPR samples is proposed. The results show that under repeated discharge pretreatments, the volume resistivity increases first and decreases afterwards when discharge magnitude is low. When partial discharge (PD) intensity increases to 100 pC, the volume and surface resistivity decrease monotonously, and the decreasing rate is positively correlated with PD intensity. During PD pre‐treatment, a white circle appears near the electrode and turns to pale yellow with PD intensity. For PD pretreatments of 50 and 100 pC, withstand voltage of EPR insulation increase first and decrease afterwards, and the downward trend is slow. For PD pre‐treatments of 200 and 400 pC, the withstand voltage decreases significantly with the degradation time. The fitting formula model shows that under the action of a strong electric field, the relationship between the reliability of EPR insulation samples, the probability and rate of molecular chain breakage, and the intensity of discharge activity is not linear.
Sonographically Detected Hepatic Portal Venous Gas – Prevalence, Causes, and Clinical Implications
Authors: Mehl Lisa , Schmidt Christa , Weidner Ulrike , Lock Guntram 
Published: April 2022

Abstract
Purpose Hepatic portal venous gas (HPVG) is a rare clinical finding, often caused by a severe underlying disease. In the literature as well as in clinical practice, HPVG is considered “signum malum” with a poor prognosis and bowel ischemia as the most common cause. Most studies are based on the results of computed tomography (CT) examinations. The aim of this retrospective study is to report on the prevalence, causes, and clinical course of HPVG in a monocentric cohort of abdominal ultrasound (US) investigations.
Materials and Methods The US database of an academic teaching hospital was searched with specific keywords (timespan 01/2000 to 12/2020). Reports, pictures, and clinical data of all cases with HPVG were re-evaluated.
Results Out of 134 804 US examinations, 8 HPVG cases were identified. There was a wide variety of underlying diseases, with mesenteric ischemia being seen in only 2 cases. 5 patients were discharged in stable condition, with 4 of them having undergone surgical treatment. 2 patients who had rejected further measures died, and one was lost to follow-up.
Discussion HPVG is a rare phenomenon in clinical US. However, ultrasonographic prevalence is comparable to the prevalence in CT studies. Underlying diseases are mostly severe, and in nearly all cases an underlying cause can be found by thorough investigation. In some cases, US may even be superior to CT scans for the detection of HPVG. Despite its rarity, every sonographer should know the typical sonographic presentation of HPVG, and appropriate images should be included in US teaching modules.
Self-expandable duodenal metal stent placement for the palliation of gastric outlet obstruction over the past 20 years
Authors: Reijm Agnes N. , Zellenrath Pauline A. , van der Bogt Ruben D. , van Driel Lydi M. J. W. , Siersema Peter D. , Bruno Marco J. , Spaander Manon C. W. 
Published: April 2022

Abstract
Introduction Duodenal stent placement is a palliative option for management of malignant gastric outlet obstruction (GOO). In the last 20 years, management of gastrointestinal cancers has considerably changed. It is unknown if these changes have affected clinical outcome of duodenal stent placement.
Methods Retrospective cohort study conducted in a tertiary referral center. Patients who underwent duodenal stent placement for GOO-symptoms due to a malignant stricture were included. Primary outcome was GOO-symptom free survival. Secondary outcomes included stent-related adverse event rates. Potential explanatory parameters such as period of stent placement (1998–2009 vs 2010–2019), prior treatments, peritoneal deposits, and stricture length were evaluated using multivariable Cox regression analysis.
Results A total of 147 patients (62 % male; median age 64 years) were included. After a median of 28 days after stent placement, 82 patients (57 %) had recurrent GOO-symptoms. GOO-symptom free survival was significantly lower in 2010–2019 (P < 0.01). Time period was the only independent predictor for reduced GOO-symptom free survival (HR 1.76, P < 0.01). Stent-related adverse event rates increased over time (1998–2009: 31 % vs 2010–2019: 37 %). Prior treatment with chemotherapy and/or radiotherapy was significantly associated with an increased risk of adverse events (OR 2.53, P = 0.02).
Conclusions Clinical outcome of duodenal stent placement did not improve over time. The decreased GOO-symptom free survival and increased adverse event rate in more recent years are probably related to the chemo- and/or radiotherapy treatment provided prior to duodenal stent placement.
Iodine-Promoted N-Acylation of Amines with Hydrazide: An Efficient Metal-Free Amidation
Authors: Ablajan Keyume , Tian Long , Zhang Qianwei 
Published: April 2022

AbstractAn efficient protocol for the amidation of hydrazide with amine has been developed by utilizing I2 as an oxidant under metal- and base-free conditions at room temperature. N-Acylation products of amines were obtained in good to excellent yields without using toxic reagents. This method is operationally straightforward and tolerates aliphatic/aromatic and primary/secondary amines with different hydrazides.
Differential Diagnosis and Management of Central Auditory Processing Disorder and Attention Deficit Hyperactivity Disorder
Authors: Chermak Gail D. , Hall James W. , Musiek Frank E. 
Published: April 2022

AbstractChildren diagnosed with attention deficit hyperactivity disorder (ADHD) frequently present difficulties performing tasks that challenge the central auditory nervous system. The relationship between ADHD and central auditory processing disorder (CAPD) is examined from the perspectives of cognitive neuroscience, audiology, and neuropsychology. The accumulating evidence provides a basis for the overlapping clinical profiles yet differentiates CAPD and ADHD as clinically distinct entities. Common and distinctive management strategies are outlined.
Abbreviations: ADHD = attention deficit hyperactivity disorder, CAPD = central auditory processing disorder
Auditory Gap Detection, Perceptual Channels, and Temporal Resolution in Speech Perception
Authors: Phillips Dennis P. 
Published: April 2022

AbstractThis article overviews some recent advances in our understanding of temporal processes in auditory perception. It begins with the premise that hearing is the online perceptual elaboration of acoustic events distributed in time. It examines studies of gap detection for two reasons: first, to probe the temporal acuity of auditory perception in its own right and, second, to show how studies of gap detection have provided new insights into the processes involved in speech perception and into the architecture of auditory spatial perceptual mechanisms. The implications of these new data for our comprehension of some central auditory processing disorders are examined.
Abbreviations: SLI = specific language impairment, VOT = voice onset time
Phenotypic determinism and stochasticity in antibody repertoires of clonally expanded plasma cells
Authors: Neumeier Daniel , Yermanos Alexander , Agrafiotis Andreas , Csepregi Lucia , Chowdhury Tasnia , Ehling Roy A. , Kuhn Raphael , Cotet Tudor-Stefan , Brisset-Di Roberto Raphaël , Di Tacchio Mariangela , Antonialli Renan , Starkie Dale , Lightwood Daniel J. , Oxenius Annette , Reddy Sai T. 
Published: April 2022

Significance
B cell clonal selection and expansion from a genetically diverse antibody repertoire guides the immune response to a target antigen. It remains unclear if clonal selection and expansion follow any deterministic rules or are stochastic with regards to phenotypic antibody properties such as antigen-binding, affinity, and epitope specificity. We perform the in-depth genotypic and phenotypic characterization of antibody repertoires following immunization in mice. We identify the degree to which clonal expansion is driven by antibody binding, affinity, and epitope specificity and as such may provide greater insight into vaccine-induced immunity.