bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bone marrow CD3+CD56+ regulatory T lymphocytes (TR3−56 cells) are inversely associated with activation and expansion of bone marrow cytotoxic T cells in IPSS‐R very‐low/low risk MDS patients
Authors: Leone Stefania , Rubino Valentina , Palatucci Anna Teresa , Giovazzino Angela , Carriero Flavia , Cerciello Giuseppe , Pane Fabrizio , Ruggiero Giuseppina , Terrazzano Giuseppe 
Published: July 2022

AbstractBackgroundEmergence of dysplastic haematopoietic precursor/s, cytopenia and variable leukaemia risk characterise myelodysplastic syndromes (MDS). Impaired immune‐regulation, preferentially affecting cytotoxic T cells (CTL), has been largely observed in MDS. Recently, we described the TR3−56 T cell subset, characterised by the co‐expression of CD3 and CD56, as a novel immune‐regulatory population, able to modulate cytotoxic functions. Here, we address the involvement of TR3−56 cells in MDS pathogenesis/progression.ObjectivesTo analyse the relationship between TR3−56 and CTL activation/expansion in bone marrow (BM) of very‐low/low‐risk MDS subjects.MethodsPeripheral blood and BM specimens, obtained at disease onset in a cohort of 58 subjects, were analysed by immune‐fluorescence and flow cytometry, to preserve the complexity of the biological sample.ResultsWe observed that a trend‐increase of BM TR3−56 in high/very‐high MDS stage, as compared with very‐low/low group, associates with a decreased activation of BM resident CTL; significant correlation of TR3−56 with BM blasts has been also revealed. In addition, in very‐low/low‐risk subjects the TR3−56 amount in BM inversely correlates with the presence of activated BM CTL showing a skewed Vβ T‐cell repertoire.ConclusionsThese data add TR3−56 to the immune‐regulatory network involved in MDS pathogenesis/progression. Better knowledge of the immune‐mediated processes associated with the disease might improve MDS clinical management.
Experimental study on fracture properties of dam concrete under post‐peak cyclic loading based on DIC and acoustic emission techniques
Authors: Bu Jingwu , Xu Huiying , Wu Xinyu , Chen Xudong , Xu Bo 
Published: July 2022

AbstractIn order to study the fracture properties of dam concrete under post‐peak cyclic loading, wedge splitting tests with three loading rates were performed on notched cubic specimens. Meanwhile, the acoustic emission (AE) and digital image correlation (DIC) technologies were used to monitor crack propagation in the specimens. As the loading rate increases, the peak load increases, and stiffness degradation increases. The cumulative AE counts show a step increasing trend and exhibit a Kaiser effect, which decreases via the post‐peak cyclic loading procedure and increases as the loading rate increases. The AE energy fluctuates markedly, and b value fluctuates frequently with an increasing loading rate. As the loading rate increases, the strain recovery rate decreases, and the permanent crack increases. The effective crack length gradually and steadily increases, and as the loading rate increases, the growth rate of the effective crack length increases.
Seasonal changes in abundance and feeding ecology of an endemic, keystone predator following the introduction of large rainbow troutOncorhynchus mykiss
Authors: Rodger Anthony W. , Starks Trevor A. 
Published: July 2022

AbstractDespite documented negative effects on aquatic ecosystems, management agencies commonly receive requests to stock non‐native rainbow troutOncorhynchus mykiss(Walbaum). We used a multinomial N‐mixture model and a suite of diet analyses to evaluate effects of large (265–530 mm) rainbow trout on reach‐scale abundance and feeding ecology of an important endemic, keystone species, Neosho bassMicropterus velox(Hubbs and Bailey).We evaluated potential changes by seasonally sampling multiple reaches of an Ozark Highlands stream before and after the introduction of 10,800 rainbow trout. Results suggest that rainbow trout altered the distribution of Neosho bass, as indicated by a negative relationship between rainbow trout density and Neosho bass abundance. Trophically, rainbow trout and Neosho bass <101 mm were equivalent, but rainbow trout diets significantly overlapped with larger Neosho bass. High rainbow trout abundance in the spring post‐stocking period changed Neosho bass diets by altering predator–prey dynamics with crayfish. Controlling for fish size revealed Neosho bass consumed larger crayfish than rainbow trout, although rainbow trout and Neosho bass between 200 and 299 mm consumed equal‐sized crayfish. Outcomes of rainbow trout stockings are likely density‐ and size‐dependent, but caution is warranted for introductions in warmwater streams.
Abiotic and biotic correlates of the occurrence, extent and cover of invasive aquatic Elodea nuttallii
Authors: Crane Kate , Kregting Louise , Coughlan Neil E. , Cuthbert Ross N. , Ricciardi Anthony , MacIsaac Hugh J. , Dick Jaimie T.A. , Reid Neil 
Published: July 2022

Abstract

Biological invasions, especially invasive alien aquatic plants, are a major and growing ecological and socioeconomic problem worldwide. Freshwater systems are particularly vulnerable to invasion, where impacts of invasive alien species can damage ecological structure and function. Identifying abiotic and biotic factors that mediate successful invasions is a management priority. Our aim was to determine the environmental correlates of Elodea nuttallii; a globally significant invasive aquatic species.

Elodea nuttallii presence/absence (occurrence), extent (patch area) and percentage cover (density) was visually assessed from a boat throughout Lough Erne (approximately 144 km2), County Fermanagh, Northern Ireland during the active summer growth season (July–September). In addition, substrate type and zebra mussel Dreissena polymorpha occurrence was recorded. Fourteen water chemistry variables were collected monthly from 12 recording stations throughout the lake during the 9 years before the survey to spatially interpolate values and establish temporal trajectories in their change. Shoreline land use was derived from CORINE land cover maps. Environmental associations between E. nuttallii, substrate, D. polymorpha, water chemistry and land use were assessed.

Elodea nuttallii occurrence was positively associated with water conductivity, alkalinity, suspended solids, phosphorus (both total and soluble) and chlorophyll‐a concentrations, but negatively associated with pH and total oxidised nitrogen. E. nuttallii patch extent and proportional cover were positively associated, to varying degrees, with the presence of D. polymorpha, biological oxygen demand, water clarity and soft substrate, but negatively associated with urban development and ammonium.

Elodea nuttallii displayed high levels of phenotypic plasticity in response to environmental variation, allowing it to adapt to a wide range of conditions and potentially gain competitive advantage over native or other invasive macrophytes.

It is evident that multiple abiotic and biotic factors, including facilitation by co‐occurring invasive dreissenid mussels, interact to influence the distribution and abundance of E. nuttallii. Thus, it is necessary to consider a more comprehensive environmental context when planning Elodea management strategies.
A Multi‐objective Optimization Approach for Disaggregating Employment Data
Authors: Ludick Chantel , van Heerden Quintin 
Published: July 2022

AbstractIn many countries, including South Africa, data on employment is rarely available on a downscaled level, such as building level, and is only available on less detailed levels, such as municipal level. The aim of this research was to develop a methodology to disaggregate the employment data that is available at an aggregate level to a disaggregate, detailed building level. To achieve this, the methodology consisted of two parts. First, a method was established that could be used to prepare a base data set to be used for disaggregating the employment data. Second, a multiobjective optimization approach was used to allocate the number of employment opportunities within a municipality to building level. The algorithm was developed using an Evolutionary Algorithm framework and applied to a case study in a metropolitan municipality in South Africa. The results showed favorable use of multiobjective optimization to disaggregate employment data to building level. By enhancing the detail of employment data, planners, policy makers, modelers and other users of such data can benefit from understanding employment patterns at a much more detailed level and making improved decisions based on disaggregated data and models.
Epistemic justice in public involvement and engagement: Creating conditions for impact
Authors: Liabo Kristin , Cockcroft Emma J. , Boddy Kate , Farmer Leon , Bortoli Silvia , Britten Nicky 
Published: July 2022

AbstractIntroductionPatient and public involvement in research is anchored in moral and epistemological rationales. Moral rationales relate to the public having a right to influence how knowledge about them is generated. Epistemological rationales relate to how research design and implementation can improve when informed by experiential, as well as technical, knowledge. In other words, public involvement can increase the epistemological resources of researchers, and contribute to research that is fit for purpose and has high external validity.MethodsThis article presents an analysis of 3 meetings and 11 interviews with public collaborators and researchers in three UK‐based health research studies. Data comprised transcripts of audio‐recorded research meetings and interviews with public collaborators and researchers. Data were first analysed to develop a data‐informed definition of experiential knowledge, then thematically to investigate how this experiential knowledge was considered and received within the research space.ResultsAt meetings, public collaborators shared their experiential knowledge as stories, comments, questions, answers and when referring to their own roles. They were aware of crossing a boundary from everyday life, and some adapted their contributions to fit within the research space. Although researchers and public collaborators made efforts to create an inclusive climate, obstacles to impact were identified.ConclusionsConsidering experiential knowledge as a boundary object highlights that this knowledge has a different form to other kinds of knowledge that contribute to research. To enable impact from experiential knowledge, researchers need to create a space where public collaborators experience epistemic justice.Patient and Public ContributionThe Peninsula Public Engagement Group (PenPEG) was involved in the planning and conceptualization of the study, including the development of the ethics application and the interview schedules. One member of this group (Richard Fitzgerald) and one from outside the group (Leon Farmer), were full members of the author team and were involved in the data analysis. Leon Farmer has since become a member of PenPEG. Richard Fitzgerald and Leon Farmer were not involved in the three research studies sampled for this study. Sadly Richard Fitzgerald died during the course of this study.
Development of international nursing standard–based curriculum for North Korean nurses
Authors: Kang Younhee , Yang Insook , Lee Eliza , Bang Chohee 
Published: July 2022

AbstractBackgroundThe types of nursing care and education have been advanced in different ways between South and North Korea during the past 73 years following division of Korea. The contents of the nursing education curriculum in North Korea do not match international standards. In preparation for the unification of the two Koreas, identifying and examining North Korean nursing education are needed.AimTo develop a curriculum for North Korean nurses to in accordance with the international nursing standards.MethodsThis study consisted of two phases. First, we reviewed the literature, analyzed in‐depth interviews with North Korean nurse defectors, and evaluated the contents and scope of nursing education in North Korea. Next, we developed a nursing education curriculum for North Korea through discussion with nursing education experts.ResultsThe nursing curriculum for North Korean nurses comprised 96 credits (21 credits for fundamental major courses and 75 credits for mandatory major courses) according to the standards of the Korea Institute of Nursing Education and Evaluation. The proposed curriculum I comprised 84.5 credits, with 14.5 credits for major courses and 70 credits for mandatory major courses. Proposed curriculum II considered the capabilities and clinical experiences of North Korean nurses and comprised 52 credits with 6 credits for major courses and 46 credits for mandatory major courses.ConclusionA nursing curriculum was proposed to match the nursing practice competencies of North Korean nurses to international standards. This curriculum can be expected to improve the quality of nursing care in North Korea, facilitate the integration of nursing workforces, and ultimately promote the health of the people during unification.Implications for Nursing PolicyThe nursing curricula proposed in this study could be a significant measure to nurture nursing manpower and contribute to narrowing the nursing gaps between South and North Korea in the process of Korea's unification.
Psychological impact of COVID‐19 on nursing personnel: A regional online survey
Authors: Hickling Matthew T. , Barnett Scott D. 
Published: July 2022

AbstractAimsThis study investigated mental health reactions to dealing with COVID‐19 in a population of nurses working in a variety of settings. The study attempted to expand our current understanding of the psychological reactions unique to nurses working during the highly stressful period of the COVID‐19 pandemic.DesignThe study used an online questionnaire design.MethodsNurses were recruited using social media via an electronic link between July and September 2020. Of them, 112 nursing professionals completed the 66‐item questionnaire.ResultsSignificant findings included the presence of moderate or greater levels of anxiety (62%), depression (31%), and posttraumatic stress disorder (15%) and with significantly greater intrusive thoughts and memories for nurses who provided direct patient care than those who did not. Nurses with a prior history of anxiety or depression were found to be at greater risk for psychological distress. Results further highlighted concerns and fears related to coronavirus in both their daily personal and professional lives.ConclusionThe use of standard, commonly used, measures of psychological disorders allow for a more precise comparison among studies both for this population at the time of the survey and over a period of time.ImpactSuggestions for helping nursing professionals identify nurses at risk and improved ways to cope and deal with adverse psychological effects are discussed.
Development of nurse‐led videoconference‐delivered cognitive behavioural therapy for domestic violence: Feasibility and acceptability
Authors: Nesset Merete Berg , Lauvrud Christian , Meisingset Anne , Nyhus Eskil , Palmstierna Tom , Lara‐Cabrera Mariela Loreto 
Published: July 2022

AbstractAimsBecause of the COVID‐19 lockdown, an internet‐based adaption of a nurse‐led cognitive behavioural group therapy (CGBT) was provided for perpetrators of domestic violence. The aim of this study was to describe the development of the therapy, examine the initial feasibility and impact on patient satisfaction of the programme and evaluate the associated patient‐reported experiences.DesignProgramme development as well as testing its feasibility and acceptability using cross‐sectional survey data.MethodsAnonymous data were collected at a university hospital in Norway between October and December 2021. Feasibility was examined by comparing the numbers of patients who agreed to participate, chose not to participate or dropped out during the intervention. There was a self‐reporting scale that evaluated patient satisfaction, and the participants were invited to make suggestions for improvement of the intervention. The results of the study are reported in accordance with the STROBE checklist.ResultsThe videoconference‐delivered CGBT was feasible. Two of the 67 patients refused to attend therapy delivered in a remote manner (3.0%), and four patients (6.0%) were classified as non‐completers. Overall, patients were satisfied with the therapy.ConclusionsThis study described a promising nurse‐led internet‐based intervention for individuals who were domestically violent and had voluntarily sought healthcare help. The participants' satisfaction with the intervention indicates its acceptability and feasibility. However, research on internet‐based cognitive behaviour therapy is still in its infancy. These results may guide the future development of internet‐based cognitive behavioural therapy (CBT) for individuals who perpetrate domestic violence. Further research is needed on the pros and cons of this mode of service delivery.ImpactThis study addressed the challenges of providing treatment for domestic violence during the COVID‐19 pandemic by examining videoconference‐delivered CBT for individuals who perpetrate domestic violence.
The practices of nurses about palliative sedation on palliative care wards: A qualitative study
Authors: Heino Linda , Stolt Minna , Haavisto Elina 
Published: July 2022

AbstractAimTo describe the practices of nurses concerning palliative sedation in palliative care wards in hospitals.DesignQualitative descriptive design.MethodsA total of 27 nurses were interviewed in six focus groups and one pair interview; the nurses worked on four palliative care wards in three Finnish hospitals and the interviews took place between May and November 2019. The data were analysed using inductive content analysis. The COREQ checklist was used as a guide for reporting this study.FindingsThree main categories describing nurses' practices concerning palliative sedation were identified: participation in palliative sedation decision‐making, sedation implementation and monitoring and information sharing and compassionate care for the patient and relatives.ConclusionsNurses play a key role throughout palliative sedation on palliative care wards in hospitals. To develop the quality of care, it is recommended to specify the role of nurses in palliative sedation, increase cooperation between nurses and physicians, and enhance palliative sedation education for nurses.
Phospholipase D1 promotes cervical cancer progression by activating the RAS pathway
Authors: Song Meiying , Meng Qianlong , Jiang Xuan , Liu Jun , Xiao Meizhu , Zhang Zhenyu , Wang Jing , Bai Huimin 
Published: July 2022

AbstractThis study aimed to further investigate the effect of PLD1 on the biological characteristics of human cervical cancer (CC) cell line, CASKI and the potential related molecular mechanism. CRISPR/Cas9 genome editing technology was used to knock out the PLD1 gene in CASKI cells. Cell function assays were performed to evaluate the effect of PLD1 on the biological function of CASKI cells in vivo and in vitro. A PLD1‐overexpression rescue experiment in these knockout cells was performed to further confirm its function. Two PLD1‐knockout CASKI cell lines (named PC‐11 and PC‐40, which carried the ins1/del4 mutation and del1/del2/ins1 mutation, respectively), were constructed by CRISPR/Cas9. PLD1 was overexpressed in these knockout cells (named PC11‐PLD1 and PC40‐PLD1 cells), which rescued the expression of PLD1 by approximately 71.33% and 74.54%, respectively. In vivo, the cell function assay results revealed that compared with wild‐type (WT)‐CASKI cells, the ability of PC‐11 and PC‐40 cells to proliferate, invade and migrate was significantly inhibited. The expression of H‐Ras and phosphorylation of Erk1/2 (p‐Erk1/2) was decreased in PC‐11 and PC‐40 cells compared with WT‐CASKI cells. PC‐11 and PC‐40 cells could sensitize CASKI cells to cisplatin. More importantly, the proliferation, migration and invasion of PC11‐PLD1 and PC40‐PLD1 cells with PLD1 overexpression were significantly improved compared with those of the two types of PLD1 knockout cells. The sensitivity to cisplatin was decreased in PC11‐PLD1 and PC40‐PLD1 cells compared with PC‐11 and PC‐40 cells. In vivo, in the PC‐11 and PC‐40 tumour groups, tumour growth was significantly inhibited and tumour weight (0.95 ± 0.27 g and 0.66 ± 0.43 g vs. 1.59 ± 0.67 g, p = 0.0313 and 0.0108) and volume (1069.41 ± 393.84 and 1077.72 mm3 ± 815.07 vs. 2142.94 ± 577.37 mm3, p = 0.0153 and 0.0128) were significantly reduced compared to those in the WT‐CASKI group. Tumour differentiation of the PC‐11 and PC40 cells was significantly better than that of the WT‐CASKI cells. The immunohistochemistry results confirmed that the expression of H‐Ras and p‐Erk1/2 was decreased in PC‐11 and PC‐40 tumour tissues compared with WT‐CASKI tumour tissues. PLD1 promotes CC progression by activating the RAS pathway. Inhibition of PLD1 may serve as an attractive therapeutic modality for CC.
ANKRD49 promotes the invasion and metastasis of lung adenocarcinoma via a P38/ATF‐2 signalling pathway
Authors: Liu Yue‐hua , Yuan Meng , Xu Bai‐xue , Gao Rui , You Yu‐jie , Wang Zhi‐xin , Zhang Yong‐cai , Guo Min , Chen Zhao‐yang , Yu Bao‐feng , Wang Qi‐Wei , Wang Hai‐long , Pang Min 
Published: July 2022

AbstractLung adenocarcinoma (LUAD) is the most challenging neoplasm to treat in clinical practice. Ankyrin repeat domain 49 protein (ANKRD49) is highly expressed in several carcinomas; however, its pattern of expression and role in LUAD are not known. Tissue microarrays, immunohistochemistry, χ2 test, Spearman correlation analysis, Kaplan–Meier, log‐rank test, and Cox's proportional hazard model were used to analyse the clinical cases. The effect of ANKRD49 on the LUAD was investigated using CCK‐8, clonal formation, would healing, transwell assays, and nude mice experiment. Expressions of ANKRD49 and its associated downstream protein molecules were verified by real‐time PCR, Western blot, immunohistochemistry, and/or immunofluorescence analyses. ANKRD49 expression was highly elevated in LUAD. The survival rate and Cox's modelling analysis indicated that there may be an independent prognostic indicator for LUAD patients. We also found that ANKRD49 promoted the invasion and migration in both in in vitro and in vivo assays, through upregulating matrix metalloproteinase (MMP)‐2 and MMP‐9 activities via the P38/ATF‐2 signalling pathway Our findings suggest that ANKRD49 is a latent biomarker for evaluating LUAD prognosis and promotes the metastasis of A549 cells via upregulation of MMP‐2 and MMP‐9 in a P38/ATF‐2 pathway‐dependent manner.