bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peer victimization, depression, and non-suicidal self-injury among Chinese adolescents: The moderating role of the 5-HTR2A gene rs6313 polymorphism
Authors: Li Meijin , Wang Huahua , Li Jingjing , Deng Yuting , Yu Chengfu 
Published: August 2022

Abstract
Background: Peer victimization is a crucial risk predictor for adolescent non-suicidal self-injury (NSSI). However, adolescent NSSI reactions to peer victimization exhibit large individual differences. This study explored whether depression mediated the association between peer victimization and adolescent NSSI, and whether this mediating path was moderated by the 5-HTR2A gene rs6313 polymorphism. Methods: A total of 667 adolescents (Mage = 12.81 ± 0.48 years) anonymously completed the questionnaires regarding peer victimization, depression, and NSSI. Genomic DNA was extracted from saliva and buccal cells from each participant. Results: The results showed that the positive relation between peer victimization and adolescent NSSI was mediated by depression. The triple interaction between peer victimization, rs6313 single nucleotide polymorphism, and sex on adolescent depression was significant. Moreover, the triple interaction between depression, 5-HTR2A gene rs6313 single nucleotide polymorphism, and sex on NSSI was also significant. In girls, homozygous CC individuals in the high peer victimization group reported significantly higher levels of depression and NSSI than participants in the low peer victimization group; the interaction, however, was not significant in boys and CT/TT genotype girls. Conclusions: These findings promote the etiological understanding of adolescent NSSI, highlighting the mediating and moderating effect of peer victimization on NSSI, and provide evidence supporting the relationship between the serotonin system and adolescent NSSI.
Do Aid for Trade Flows Help Reduce the Shadow Economy in Recipient Countries?
Authors: GNANGNON Sèna Kimm 
Published: August 2022

Abstract
The present analysis has assessed the effect of Aid for Trade (AfT) flows on the shadow economy using an unbalanced panel dataset of 106 countries over the period from 2002 to 2015. Empirical results obtained by means of the two-step system generalized method of moments, show that higher AfT flows are associated with a fall in the size of the shadow economy, with less developed countries experiencing a greater negative effect of AfT flows on the shadow economy than relatively advanced countries among recipient countries of AfT flows. AfT interventions reduce the size of the shadow economy in countries that face high trade costs as well as in those that experience a depreciation of the real exchange rate. Finally, AfT interventions reduce the size of the shadow economy in countries that improve the economic complexity level.JEL Classification: F14; F35; O17.
Over expression of recombinant Staphylokinase and reduction of inclusion bodies using IPTG as inducer in E. coli BL21 DE3
Authors: Muttar Barakat Abdul Razzaq , Panchal Rakesh Kumar R. 
Published: August 2022

Abstract
A previous research and studies have established the significance of describing Staphylokinase as a thrombolytic treatment, particularly in patients with cardiovascular disease, stroke, and other life-threatening disorders. Staphylokinase plays a significant part in the coagulation process through formation plasmin-Staphylokinase complex on the surface of the clot which activates plasminogen is generated by some strains of Staphylococcus aureus. The publication described the use of the E. coli strain BL12DE3 to produce the protein via the pET21a transporter is predicated on the use of a chemical stimulator (IPTG) which plays an important role in regulating protein recombinant by stimulating protein synthesis. Strain BL12DE3 activates T7 polymerase, encoding LacI via the pET21a transporter. It has been demonstrated that the IPTG inducer achieves the maximum level of protein expression in the shortest amount of time. A Staphylokinase was generated as a soluble protein in excess of 45% by SDS-PAGE and subsequently purified by chromatography.
Allee effect driven by predation: A study of Gause type predator-prey model
Authors: Sasmal Sourav Kumar , Pal Saheb , Pal Nikhil , Takeuchi Yasuhiro 
Published: August 2022

Abstract
Reduced population growth at low population density has important implications for conservation, colonization success, and wildlife management. In this context, the Allee effect, i.e., the positive relationship between per capita growth rate and biomass at small population density, is a crucial biological phenomenon since it is directly related to population extinction. The present paper deals with a two-species interacting model with predator-prey relationship, where prey population experiences mate finding Allee effect caused by predator. Here we assume that an individual's searching efficiency decreases linearly with predator density due to predation, and investigate how predation intensity affects predator-prey dynamics. Moreover, we consider the Monod-Haldane type functional response for predator-prey interactions, which shows group defense of prey against the predator. We provide a detailed mathematical analysis, including positivity and boundedness of solutions, all biologically feasible equilibria, and their local and global stabilities. From our detailed mathematical analysis, we can observe that when the system carrying capacity is low, at most one interior equilibrium exists, and system dynamics is simple, compared to the case with high carrying capacity, in which multiple coexistence equilibria may exist. We discuss three co-dimension one bifurcations mathematically, e.g., Hopf-bifurcation, transcritical bifurcation, saddle-node bifurcation. We notice multi-stability in our system, where the system can be bi-stable when there are two-interior equilibria with high carrying capacity. However, a unique attractor exists when there is only a single interior equilibrium, and both populations persist. We perform extensive numerical simulations by varying two parameters simultaneously and explore how system dynamics are complex when carrying capacity is high compared to low carrying capacity. Moreover, in the numerical section, we discuss other important biological phenomena, e.g., the paradox of enrichment, bubbling, etc.
Ventricular entry during surgical resection is associated with intracranial leptomeningeal dissemination in glioblastoma patients
Authors: Battista Francesca , Muscas Giovanni , Dinoi Francesca , Gadda Davide , Puppa Alessandro Della 
Published: August 2022

Abstract
PURPOSE
Glioblastoma (GBM) is associated with a poorer prognosis when leptomeningeal dissemination (LMD) is reported. Recently, the role of both ventricular entry (VE) during surgery and subventricular zone (SVZ) localization of tumors in promoting LMD in GBM patients has been debated. This article investigates the role of leptomeningeal dissemination (LMD) after surgical exeresis of GBM of VE and SVZ location of the tumor.
METHODS
We conducted a retrospective analysis of GBMs operated on at our Institution between March 2018 and December 2020. We collected pre-and post-surgical images, anamnestic information, and surgical reports.
RESULTS
Two hundred cases were collected. The GBM localization was periventricular in 69,5% of cases, and there was a VE during the surgical procedure in 51% of cases. The risk of post-surgical LMD in the case of VE was 16%. The rate of LMD was higher in the case of VE than not-VE (27,4% vs. 4%, p < 0.0001). The rate of LMD in periventricular GBM was 19% (p = 0.1131).
CONCLUSION
According to our data, VE is an independent factor associated with a higher rate of post-surgical LMD, and the periventricular localization is not independently correlated to this negative outcome. Neurosurgeons should avoid VE when possible. The decision should be assessed case by case, and the correct surgical strategy should be founded on balancing the need for maximal EOR and the risks associated with VE.
Presentation of a new wood test specimen to investigate the fracture behavior of orthotropic materials under mixed-mode I/II loading
Authors: Fakoor Mahdi , Khali Zahra 
Published: August 2022

Abstract
In this paper, a new test specimen as "Center Notched Disk Tension (CNDT)" to study mode I and II stress intensity factors (SIF’s) in orthotropic materials is presented. In the field of Fracture Mechanics, mode I and II SIF’s play an important role to describe the stress distribution. In CNDT specimens, for crack along the fibers, mode mixity is created by changing the loading direction. Also, by changing the crack-fibers direction for the desired crack-load, various mode mixity can be created. For desired crack-fiber angle, by changing the loading share, different mode mixities are created. Thus, the crack kink changes the loading shares. In this analysis, a comprehensive study is performed on the influence of crack-fibers orientation and crack-loading direction on SIF’s. For the first time, SIF’s are presented for cracked orthotropic specimens in different crack-fibers and crack-loading directions. SIF’s are estimated using the Finite Element Method (FEM). The presented specimen is standardized using a unite load in different directions. The crack length is assumed to be constant. Analytical relationships are also presented for the crack tip parameters in mode I and II. Pure mode I and II conditions are investigated based on SIF’s. Due to the difficulty of establishing pure mode II conditions, it is possible to establish pure mode II conditions at certain crack-fiber and load-crack angles. In these situations, mode II SIF’s can be extracted. The obtained results are validated using reported results in the literature.
Analysis of object detection models and their sampling features for Time series Forecasting Methods
Authors: Mani Pradeep 
Published: August 2022

Abstract
Sampling Features that detect the object model in a time series method for analyse the forecasting of image model that may be uni-variate or multi-variate for preprocessing a model in time series that it can break forecasting, the data should be predicted with the area should distribute the level in the region. Forecast Prophecy prediction denotes the sequence model indicating the time of an event in a series. When there is an insufficient information in a traditional pattern predictive model in a block of data append with this model to fissure the data in the various level it can be monitored by several features when there is a superfluous model data in the applicable area with the inclination epoch section, continual integrant, remnant constituent, obstruct area to be monitored with the order for data availability in the region. The object detected with the variational factor should identify with an accuracy of 0.25 in the existing system. In Proposed model IoU ≥0.5 and the improvement factors will observed in FLOPs in a different computational layer categorized model 23.5%, 46.7 % and 50.0 % in a respective ratio in this method train the model with better accuracy and high precision rate.
High-resolution crystal structure of a metabolic switch protein in a complex with monomeric c-di-GMP reveals a potential mechanism for c-di-GMP dimerization
Authors: Dubey Badri Nath , Shyp Viktoriya , Fucile Geoffrey , Jenal Urs , Schirmer Tilman 
Published: August 2022

Abstract
Bacterial second messengers c-di-GMP and (p)ppGpp have broad functional repertoires ranging from growth and cell cycle control to the regulation of biofilm formation and virulence. The recent identification of SmbA, an effector protein from Caulobacter crescentus that is jointly targeted by both signaling molecules, has opened up studies on how these global bacterial networks interact. C-di-GMP and (p)ppGpp compete for the same SmbA binding site, with a dimer of the former ligand inducing a conformational change of loop 7 leading to downstream signaling. Here, we report a crystal structure of a partial loop 7 deletion mutant, SmbA∆loop in complex with c-di-GMP determined at 1.4 Å resolution. SmbA∆loop binds monomeric c-di-GMP strengthening the view that loop 7 is required for c-di-GMP dimerization. In the crystal, SmbA∆loop forms a 2-fold symmetric dimer via isologous interactions with the two symmetric halves of c-di-GMP. Structural comparisons of SmbA∆loop with wild-type SmbA in complex with dimeric c-di-GMP or ppGpp support the idea that loop 7 is critical for SmbA function by interacting with downstream partners. These results underscore the flexibility of c-di-GMP in binding to the symmetric interface between protein subunits. It is envisaged that such isologous interactions of c-di-GMP will be observed in hitherto unrecognized targets.
PRSS23 (Serine Protease 23) is required for the progression of gastric cancer
Authors: Wang Mengshan , Li Bing , He David C 
Published: August 2022

Abstract
PRSS23 (Serine Protease 23) is a vital serine protease that is related to various types of cancers. However, the correlation between PRSS23 and gastric cancer remains unclear. In our study, our objective is to identify the key biomarkers and signaling pathways by analyzing the RNA-seq data. The GSE204725 was created by the Illumina HiSeq 4000 (Homo sapiens). The gene enrichment study indicated the cAMP signaling pathway and Calcium signaling pathway are the main biological processes during the knockdown of PRSS23 in gastric cancer. Moreover, we identified several interactive genes including NCAM1, HIST1H2BD, VWF, HIST1H2BL, GATA1, RYR2, CHRD, PDE4B, HIST1H4B, and HIST2H4B. Our study may provide new knowledge on the treatment of gastric cancer.
An analysis of lactate dehydrogenase (LDH) levels in advanced stage IV melanoma of the skin: prognostic capabilities and demographic variability
Authors: Desai Amar D. , Chinta Sree , Yeh Christopher , Shah Vraj P. , Shah Radhika , Paskhover Boris , Schwartz Robert A. 
Published: August 2022

Abstract
Background
Despite its limitations, in part due to decreased specificity in advanced disease, serum lactate dehydrogenase (LDH) is one of few serum factors used in cancer staging.
Objectives
This study quantifies the predictive capabilities of LDH in stage IV melanoma of the skin and explores the validity of suggested demographic discrepancies which may exist in its use.
Methods
The 1975–2017 Surveillance Epidemiology and End Results (SEER) database was queried for stage IV cutaneous melanoma cases. Demographic characteristics were compared between LDH groups using chi-square and t-tests. Subsequent Cox multivariable regression was performed to assess survival differences.
Results
334 cases of stage IV cutaneous melanoma (average age: 63.0 years) with measured serum LDH levels were identified. Of these patients, 150 (44.9%) had normal LDH, 112 (33.5%) had LDH < 1.5x upper limit of normal (ULN), 57 (17.1%) had LDH 1.5-10x ULN, and 15 (4.5%) had LDH > 10x ULN. Lower incomes were associated with higher LDH; individuals with incomes <$50,000 had the greatest proportion of LDH 10x ULN (19.2%; p = 0.0031). LDH > 10x ULN also had the lowest proportion of White patients (p = 0.04). On Cox multivariable survival analysis, increasing LDH levels showed increased risk of death (LDH < 1.5x ULN: HR = 2.05, p = 0.01; LDH 1.5-10x ULN: HR = 1.46, p < 0.001; LDH > 10x ULN: HR = 5.91, p < 0.001).
Conclusion
This study reaffirms the utility of LDH as a significant predictor of mortality with incremental severity, suggesting possible use for mortality projections. We note that Black patients and those with lower incomes may be more likely to have an elevated LDH. Older age groups and presence of ulceration among patients with stage IV melanoma were also associated with a greater risk of mortality.
A In-solution Snapshot of SARS-COV-2 Main Protease Maturation Process and Inhibition
Authors: Noske Gabriela , Song Yun , Fernandes Rafaela , Chalk Rod , Elmassoudi Haitem , Koekemoer Lizbé , Owen C David , Oliva Glaucius , Godoy Andre 
Published: August 2022

Abstract
Mpro is the main protease of SARS-CoV-2. Its dimeric form is responsible for cleavage of the viral polyprotein at 11 sites, including its own N- and C-termini. Mpro self-cleavage is called maturation, and it is crucial for enzyme dimerization and activity. Recently, we combined x-ray crystallography with biochemical characterization to depict key steps of the maturation process. A single mutant C145S version of Mpro linked to nsp4 cleavage sequence was introduced, allowing us to monitor enzyme shifts between oligomeric states in a human timescale. In here, we used C145S Mpro to study the structure and dynamics of N-terminal cleavage in solution. Native mass spectroscopy analysis showed that mixed oligomeric states are composed of cleaved and uncleaved particles, indicating that N-terminal processing is not critical to oligomerization. A 3.5 Å cryo-EM structure provides details of Mpro N-terminal cleavage in solution, and a glimpse in the dynamic of the active sites outside the constrains of crystal environment. We explored how different classes of inhibitors shift the balance between oligomeric states of Mpro. While non-covalent inhibitor MAT-POS-e194df51-1 prevents oligomerization, we discovered that the covalent inhibitor Nirmatrelvir induces the conversion of monomers into dimers, even with intact N-terminal. Our data indicates that the Mpro dimerization is triggered by the induced fit caused by the covalent linkage during substrate processing rather than the N-terminal processing.
Origin of Screen-printed Metal Contact Losses in Crystalline Silicon Solar Cells
Authors: Jeong Myeong Sang , Lee Sang Hee , Choi Sungjin , Min Kwan Hong , Lee Jong Hoon , Kang Min Gu , Jeong Kyung Taek , Kwak Sang Kyu , Song Hee-eun , Lee Tae Kyung , Park Sungeun 
Published: August 2022

Abstract
Crystalline silicon solar cells are considered mainstream products in the photovoltaic market. To further improve their performance, it is important to maintain good contact between metal electrodes and the Si surface because the state of the metal–Si interface affects the saturation current density J0.metal and open-circuit voltage. Moreover, the large saturation current density strongly attributes to the performance degradation of solar cells. Therefore, the interfacial structure formed by the metal electrodes and Si surface must be examined to minimize the J0.metal value. In this study, we investigated the formation mechanism of Ag crystallites on the surface of Si emitter layer in screen-printed Ag paste. Interestingly, J0.metal was minimized by Ag epitaxial growth, which was verified using an atomic-scale approach. Furthermore, the effect of P doping on the Ag–Si interfacial structure reduced J0.metal. Our study can provide new insights into the origin of J0.metal for realizing high-performance solar cells.
Micro-batch and Data Frequency for Stream Processing on Multi-cores
Authors: Garcia Adriano Marques , Griebler Dalvan , Schepke Claudio , Fernandes Luiz Gustavo 
Published: August 2022

Abstract
Latency or throughput are often critical performance metrics in stream processing. Applications' performance can fluctuate depending on the input stream. This unpredictability is tied to variations in data arrival frequency, data size, complexity, and other factors. Researchers are constantly investigating new ways to mitigate the impact of these variations on performance with self-adaptive techniques involving elasticity or micro-batching. However, there is a lack of benchmarks capable of creating test scenarios to further evaluate these techniques. This work extends and improves the SPBench benchmarking framework to support dynamic micro-batching and data stream frequency management. We also propose a set of algorithms that generate the most commonly used frequency patterns for benchmarking stream processing in related work. It allows the creation of a wide variety of test scenarios. To validate our solution, we use SPBench to create custom benchmarks and evaluate the impact of micro-batching and data stream frequency on the performance of Intel TBB and FastFlow. These are two libraries that leverage stream parallelism for multi-core architectures. Our results demonstrated that most test cases benefited from micro-batches, especially high throughput applications with ordering constraints. For different data stream frequency configurations, TBB ensured the lowest latency, while FastFlow assured higher throughput in shorter pipelines.
Single-crystal UV/Vis optical absorption spectra of almandine-bearing and spessartine garnet: Part II. An analysis of the spin-forbidden bands of Fe2+, Mn2+, and Fe3+
Authors: Taran Michail N. , Geiger Charles A. , Vyshnevskyi Oleksii A. , Rossman George R. 
Published: August 2022

Abstract
The UV/Vis single-crystal absorption spectra of two almandine-bearing and several spessartine garnets were measured and their respective Fe2+ and Mn2+ spin-forbidden electronic transitions analyzed. Spin-forbidden bands of Fe3+ are also considered, because many aluminosilicate garnets contain some Fe3+. The spectra of the almandine-bearing garnets were recorded at room temperature between about 10 000 and 30 000 cm–1. The spectrum of a nearly end-member spessartine (97 mol% Mn32+Al2Si3O12) was measured between about 15 000 cm–1 and 30 000 cm–1 at room temperature and 78 K, the latter for the first time. The 78 K spectrum shows absorption features not observed at room temperature. Five additional spessartine-rich garnets with different Mn2+/(Mn2+ + Fe2+) ratios, and two with unusual chemistries, were recorded up to 26 000 cm–1. The spectra of the two almandine-bearing garnets agree well with published results and show several overlapping Fe2+/3+ bands located between about 14 000 and 25 000 cm–1. The spectra were deconvoluted to gain more insight into the electronic transition behavior. These results, together with an analysis of other measured spectra, reveal several absorption features that were previously unrecognized or misassigned. The spectrum of spessartine shows several Mn2+ bands, and most are clearly spaced from one another. A synthesis of various UV/Vis spectroscopic results is made and assignments for the Fe2+/3+ and Mn2+ bands are attempted. The intensities of the Mn2+ spin-forbidden bands and the ligand → metal charge edge observed in the various spessartine spectra are discussed. Spectra of almandine and spessartine have been interpreted using Tanabe-Sugano diagrams that are constructed for cations in octahedral coordination, point symmetry Oh. However, such analysis does not appear to be fully successful because Fe2+ and Mn2+ in garnet have triangular dodecahedral coordination with point symmetry D2. The interpretation of the spectrum of spessartine is especially problematic. An analysis shows that published model calculations of Fe2+ electronic transition energies in garnet are not in good agreement with each other and are also not in full agreement with experimental spectra. First principles calculations are needed to better understand the spin-forbidden transitions of Fe2+, Fe3+, and Mn2+ in garnet.