bluuebunny/crossref_metadata_2025_split huggingface dataset:
Small RNAs in the Antarctic bacterium Pseudomonas extremaustralis responsive to oxygen availability and oxidative stress
Authors: Solar Venero Esmeralda C. , Matera Gianluca , Vogel Jörg , López Nancy I. , Tribelli Paula M. 
Published: June 2022

SummaryBacterial small non‐coding RNAs (sRNAs) play key roles as genetic regulators, mediating in the adaptability to changing environmental conditions and stress responses. In this work, we analysed putative sRNAs identified by RNA‐seq experiments in different aeration conditions in the extremophile bacterium P. extremaustralis. These analyses allowed the identification of 177 putative sRNAs under aerobiosis (A), microaerobiosis (M) and microaerobiosis after H2O2 exposure (m‐OS). The size and transcription profile of eight sRNAs with differential expression were verified by Northern blot. sRNA40, with unknown function but conserved in other Pseudomonas species, was selected to perform overexpression experiments followed by RNA‐seq analysis. The overexpression of sRNA40 in P. extremaustralis resulted in significant expression changes of 19 genes with 14 differentially upregulated and five downregulated. Among the upregulated genes, eight transcripts corresponded to components of secretion systems, such as gspH, gspK, and gspM, belonging to the Type II secretion system, and rspO and rspP from Type III secretion system. Our results showed a novel sRNA which expression was triggered by low oxygen levels, and whose overexpression was associated with upregulation of selected components of protein secretion systems.
Effectiveness of tongue‐tie assessment tools in diagnosing and fulfilling lingual frenectomy criteria: a systematic review
Authors: Hatami A , Dreyer CW , Meade MJ , Kaur S 
Published: June 2022

ABSTRACTIt is unclear how effective tongue‐tie classification assessment tools are in diagnosing symptomatic tongue‐tie and fulfilling lingual frenectomy criteria. The purpose of this systematic review is to determine and evaluate any association between tongue‐tie severity, as measured by pre‐treatment assessment tools, and post‐operative outcome following tongue‐tie division. PubMed, EMBASE, and the Cochrane search engines were used to retrieve articles published between 1947 and 2021. Included studies consisted of patients with symptomatic tongue‐tie, assessment by either the Coryllos, Kotlow, or Hazelbaker Assessment Tool for Lingual Frenulum Function (HATLFF) classification tool, and tongue‐tie division. A total of 205 abstracts were identified; 31 studies met the criteria for a full‐text review, of which, only 14 studies met the criteria for data extraction and analysis. Six studies used the HATLFF, 2 studies used the Kotlow, 5 studies used the Coryllos, and 1 study used a combination of both Kotlow and Coryllos methods. Significant heterogeneity was evident across all studies. No statistical correlation between the two variables could be determined. Although tongue‐tie division procedures appear to provide benefits in breastfeeding and speech, there are no data to suggest a statistically significant association between the severity of tongue‐tie, and the correct identification of patients who would benefit from tongue‐tie division. © 2022 Australian Dental Association.
What did we learn in 35 years of research on nutrition and supplements for age‐related macular degeneration: a systematic review
Authors: Pameijer Els M. , Heus Pauline , Damen Johanna A. A. , Spijker René , Hooft Lotty , Ringens Peter J. , Imhof Saskia M. , van Leeuwen Redmer 
Published: June 2022

AbstractThe aim of this paper is to summarize all available evidence from systematic reviews, randomized controlled trials (RCTs) and comparative nonrandomized studies (NRS) on the association between nutrition and antioxidant, vitamin, and mineral supplements and the development or progression of age‐related macular degeneration (AMD). The Cochrane Database of Systematic Reviews, Cochrane register CENTRAL, MEDLINE and Embase were searched and studies published between January 2015 and May 2021 were included. The certainty of evidence was assessed according to the GRADE methodology. The main outcome measures were development of AMD, progression of AMD, and side effects. We included 7 systematic reviews, 7 RCTs, and 13 NRS. A high consumption of specific nutrients, i.e. β‐carotene, lutein and zeaxanthin, copper, folate, magnesium, vitamin A, niacin, vitamin B6, vitamin C, docosahexaenoic acid, and eicosapentaenoic acid, was associated with a lower risk of progression of early to late AMD (high certainty of evidence). Use of antioxidant supplements and adherence to a Mediterranean diet, characterized by a high consumption of vegetables, whole grains, and nuts and a low consumption of red meat, were associated with a decreased risk of progression of early to late AMD (moderate certainty of evidence). A high consumption of alcohol was associated with a higher risk of developing AMD (moderate certainty of evidence). Supplementary vitamin C, vitamin E, or β‐carotene were not associated with the development of AMD, and supplementary omega‐3 fatty acids were not associated with progression to late AMD (high certainty of evidence). Research in the last 35 years included in our overview supports that a high intake of specific nutrients, the use of antioxidant supplements and adherence to a Mediterranean diet decrease the risk of progression of early to late AMD.
Editorial: COVID‐19 vaccines are safe and effective in patients with inflammatory bowel disease—but many unanswered questions remain
Authors: Benchimol Eric I. , Kuenzig M. Ellen 
Published: June 2022

LINKED CONTENTThis article is linked to Bhurwal et al papers. To view these articles, visit https://doi.org/10.1111/apt.16913 and https://doi.org/10.1111/apt.16935
Letter: non‐selective beta‐blockers in cirrhosis‐effect beyond portal hypertension
Authors: Chauhan Ashish , Bhatia Aakanksha 
Published: June 2022

LINKED CONTENTThis article is linked to Dunne et al papers. To view these articles, visit https://doi.org/10.1111/apt.16901 and https://doi.org/10.1111/apt.16982
Editorial: seeing the light at the end of gastroscope—Complications after elective procedures are ready for NICE, bright decision aid tools
Authors: Shiratori Yasutoshi , Miller Steven D. , Hutfless Susan 
Published: June 2022

LINKED CONTENTThis article is linked to Crooks et al papers. To view these articles, visit https://doi.org/10.1111/apt.16946 and https://doi.org/10.1111/apt.16987
Letter: non‐invasive prediction models to exclude cirrhosis in NAFLD—not everyone fits the mould. Authors' reply
Authors: Brandman Danielle , Boyle Marie , McPherson Stuart , Van Natta Mark L. , Sanyal Arun J. , Kowdley Kris , Neuschwander‐Tetri Brent , Chalasani Naga , Abdelmalek Manal F. , Terrault Norah A. , McCullough Art , Bettencourt Ricki , Caussy Cyrielle , Kleiner David E. , Behling Cynthia , Tonascia James , Anstee Quentin M. , Loomba Rohit 
Published: June 2022

LINKED CONTENTThis article is linked to Brandman et al papers. To view these articles, visit https://doi.org/10.1111/apt.16874 and https://doi.org/10.1111/apt.16931
Letter: paying attention to the comorbidities or extraintestinal complications in patients with inflammatory bowel disease during the COVID‐19 pandemic—authors’ reply
Authors: Hadi Yousaf , Kochhar Gursimran S. 
Published: June 2022

LINKED CONTENTThis article is linked to Yuan et al papers. To view these articles, visit https://doi.org/10.1111/apt.16915
Editorial: seeing the light at the end of gastroscope ‐ complications after elective procedures are ready for NICE, bright decision aid tools ‐ authors' reply
Authors: West Joe , Card Timothy R. , Crooks Colin J. 
Published: June 2022

LINKED CONTENTThis article is linked to Crooks et al papers. To view these articles, visit https://doi.org/10.1111/apt.16946 and https://doi.org/10.1111/apt.16961
Towards an agenda for oral history and geography: (Re)locating emotion in family narratives of domestic abuse in 1970s East Kilbride
Authors: Levine Hampton Rosie 
Published: June 2022

AbstractThis paper sets out a research agenda for oral history in/and geography, with a particular focus on emotional historical geographies. With three families' relocation from inner‐city Glasgow to the new town of East Kilbride as the empirical backdrop, I argue that oral history methodology is uniquely well placed to capture both the emotionality and spatiality of historical narratives. Whilst previous reviews across geography and oral history theory have considered important emotional markers such as tone of voice, the expression of feeling and body language, I intervene by focusing on different narrative strategies employed throughout the interview and argue that the geographical remit of an interviewee's memories is inseparable from their emotional brevity. The focus of the paper is therefore threefold. Firstly, I consider the subjective temporalities and spatialities that oral history narrators employ when seeking composure, considering how they might “re‐place” their narratives (and thus themselves) in the interview setting. Secondly, I argue that how interviewees might “re‐place” themselves is inextricable, and as such so is any emotional reading of the interview, from the inter‐subjective relationship produced by such an encounter. Finally, I explore the implications of this methodology for carefully elaborating on the intersection between the intimate geographies of the home, and geographies of violence. How the interviewees witnessed domestic abuse among their families was moulded by the context of their relocation. Moreover, the inter‐subjective relationship, an analysis of which I argue is crucial to the findings of the paper, was also distinctly moulded by that same family context – as I was conducting these interviews with members of my own family.
Altered gut microbiota and metabolites profile are associated with reduced bone metabolism in ethanol‐induced osteoporosis
Authors: Liu Zhao , Xu Xilin , Shen Yiwei , Hao Yuanyuan , Cui Wenwen , Li Wenyan , Zhang Xin , Lv Hang , Li Xiaodong , Hou Yunlong , Zhang Xiaofeng 
Published: June 2022

AbstractObjectiveChronic heavy drinking causes ethanol‐induced osteoporosis (EIO). The present study aimed to explore the role of GM in EIO.Material and MethodsA rat EIO model was established by chronic ethanol intake. Taking the antibiotic application as the matched group of dysbacteriosis, an integrated 16S rRNA sequencing and liquid chromatography–tandem mass spectrometry‐based metabolomics in serum and faeces were applied to explore the association of differential metabolic phenotypes and screen out the candidate metabolites detrimental to ossification. The colon organoids were used to track the source of 5‐HT and the effect of 5‐HT on bone formation was examined in vitro.ResultsCompared with antibiotics application, ethanol‐gavaged decreased the BMD in rats. We found that both ethanol and antibiotic intake affected the composition of GM, but ethanol intake increased the ratio of Firmicutes to Bacteroidetes. Elevated serotonin was proved to be positively correlated with the changes of the composition of GM and faecal metabolites and inhibited the proliferation and mineralization of osteogenesis‐related cells. However, the direct secretory promotion of serotonin was absent in the colon organoids exposed to ethanol.ConclusionThis study demonstrated that ethanol consumption led to osteoporosis and intestinal‐specific dysbacteriosis. Conjoint analysis of the genetic profiles of GM and metabolic phenotypes in serum and faeces allowed us to understand the endogenous metabolite, 5‐HT, as detrimental regulators in the gut‐bone axis to impair bone formation.
An association study of ABCG2 rs2231142 on the concentrations of allopurinol and its metabolites
Authors: Pilon Marc‐Olivier , Leclair Grégoire , Oussaïd Essaïd , St‐Jean Isabelle , Jutras Martin , Gaulin Marie‐Josée , Mongrain Ian , Busseuil David , Rouleau Jean Lucien , Tardif Jean‐Claude , Dubé Marie‐Pierre , de Denus Simon 
Published: June 2022

AbstractABCG2 is a gene that codes for the human breast cancer resistance protein (BCRP). It is established that rs2231142 G>T, a single nucleotide polymorphism of the ABCG2 gene, is associated with gout and poor response to allopurinol, a uric acid‐lowering agent used to treat this condition. It has also been suggested that oxypurinol, the primary active metabolite of allopurinol, is a substrate of the BCRP. We thus hypothesized that carrying the rs2231142 variant would be associated with decreased oxypurinol concentrations, which would explain the lower reduction in uric acid. We performed a cross‐sectional study to investigate the association between the ABCG2 rs2231142 variant and oxypurinol, allopurinol, and allopurinol riboside concentrations in 459 participants from the Montreal Heart Institute Hospital Cohort. Age, sex, weight, use of diuretics, and estimated glomerular filtration rate were all significantly associated with oxypurinol plasma concentration. No association was found between rs2231142 and oxypurinol, allopurinol and allopurinol riboside plasma concentrations. Rs2231142 was not significantly associated with daily allopurinol dose in the overall population, but an association was observed in men, with T carriers receiving higher doses. Our results do not support a major role of ABCG2 in the pharmacokinetics of allopurinol or its metabolites. The underlying mechanism of the association between rs2231142 and allopurinol efficacy requires further investigation.
Persistent disparities in diabetes medication receipt by socio‐economic disadvantage in Australia
Authors: Morton Jedidiah I. , Ilomӓki Jenni , Magliano Dianna J. , Shaw Jonathan E. 
Published: June 2022

AbstractBackgroundIt is unknown how use of newer glucose‐lowering drugs (GLDs) has changed in Australia following the publication of clinical trials demonstrating definitive clinical advantages for glucagon‐like peptide‐1 receptor agonists (GLP‐1 RAs) and sodium‐glucose co‐transporter 2 inhibitors (SGLT2is), and whether this varies by socio‐economic disadvantage.MethodsWe included 1,064,645 people with type 2 diabetes registered on the National Diabetes Services Scheme. This cohort was linked to the Pharmaceutical Benefits Scheme database to evaluate trends in diabetes medication receipt and variation by socio‐economic disadvantage between 2013 and 2019.ResultsThe proportion of people with type 2 diabetes receiving ≥3 GLDs concurrently increased from 12% in 2013 to 25% in 2019. By 2019, 6% of people with diabetes were receiving a GLP‐1 RA and 21% an SGLT2i. Disparities in receipt of GLP‐1 RAs and SGLT2is by socio‐economic disadvantage decreased over time (ORs for most vs. least disadvantaged quintile were 0.80 [0.77–0.85] and 0.87 [0.82–0.94] in 2014 and 0.95 [0.92–0.98] and 1.07 [1.05–1.09] in 2019 for GLP‐1 RAs and SGLT2is, respectively). However, people in more disadvantaged areas were more likely to receive multiple GLDs. After stratifying by number of concurrent GLDs received, people in more disadvantaged areas were less likely to receive GLP‐1 RAs and SGLT2is in 2019 (ORs for most vs. least disadvantaged: 0.81 [0.78–0.84] and 0.90 [0.87–0.93] for people receiving ≥3 GLDs, respectively).ConclusionsAfter controlling for intensity of glucose‐lowering therapy, people in more disadvantaged areas were less likely to receive cardioprotective GLDs, although disparities decreased over time.
Real world hypoglycaemia related to glucose variability and Flash glucose scan frequency assessed from global FreeStyle Libre data
Authors: Riveline Jean‐Pierre , Wojtusciszyn Anne , Guerci Bruno , Alves Clara , Kao Kalvin , Xu Yongjin , Dunn Timothy C. 
Published: June 2022

AbstractAimFlash glucose monitoring provides a range of glucose metrics. In the current study, we aim to identify those that indicate that glycaemic targets can be consistently met and contrast the total (t‐CV) and within‐day coefficient of variation (wd‐CV) to guide the assessment of glucose variability and hypoglycaemia exposure.MethodsDe‐identified data from Flash readers were collected. The readers were sorted into 10 equally sized groups of scan frequency followed by quartiles of estimated A1c (eA1c). A similar grouping was performed for the total coefficient of variation (t‐CV) and within‐day coefficient of variation (wd‐CV). In addition, analysis of the association of time below 54 mg/dl and glucose variability measured by t‐CV and wd‐CV was performed.ResultsThe dataset included 1 002 946 readers. Readers sorted by 10 equal groups of scan rate and quartiles by eA1c, t‐CV and wd‐CV represented 25 074 readers per group. The association of lower eA1c with higher time in range and reduced time above range was clear. The correlation of eA1c quartiles and time below range was not consistent. An association between glucose variability and hypoglycaemia was found. Both wd‐CV and t‐CV were associated with time below range. For achieving the consensus target of <1% time below 54 mg/dl, the associated wd‐CV and t‐CV values were 33.5% and 39.5%, respectively.ConclusionsThe type of CV reported by the different continuous glucose monitoring systems should be acknowledged. CV <36% might not be adequate to ensure low hypoglycaemia exposure. To our knowledge, the majority of continuous glucose monitoring reports the t‐CV. Appropriate thresholds should be used to identify patients that would probably meet time below range targets (t‐CV <40% or wd‐CV <34%).
Resistance in lentil (Lens culinaris) genetic resources to the pea aphid (Acyrthosiphon pisum)
Authors: Das Shimul , Porter Lyndon D. , Ma Yu , Coyne Clarice J. , Chaves‐Cordoba Bernardo , Naidu Rayapati A. 
Published: June 2022

AbstractThe pea aphid (PA), Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae), can cause complete yield loss in lentil, Lens culinaris Medik. (Fabaceae, Leguminosae), if not managed properly. Determining lentil genetic resources with resistance to PA is important for integrated pest management (IPM) in production areas worldwide. The objectives of this study were to identify genetic resources and genes associated with resistance to PA in 188 lines of the USDA Lentil Single Plant Core Collection. A cluster analysis of the mean number of adults, nymphs, and total aphids that developed on lentil lines 10 days after infestation with a single aphid identified four clusters associated with aphid resistance: 20 lines were considered resistant, 99 moderately resistant, 51 moderately susceptible, and 18 susceptible. PIs 329157 LSP and 432085 LSP, originating from Iran, demonstrated the best resistance when assessing antibiotic and antixenotic properties. Genotyping‐by‐sequencing (GBS)‐based single nucleotide polymorphisms (SNPs) were used for genome‐wide association mapping to locate genes or quantitative trait loci (QTL) associated with PA resistance traits, in which six, four, and three SNPs were associated with low numbers of PA adults, nymphs, and total aphids that developed on lentil lines, respectively. Fifteen candidate genes were identified relating to PA resistance. The results can effectively be used in molecular lentil breeding programs to target specific genomic regions associated with PA resistance, as well as in traditional breeding, using lines identified as highly PA‐resistant as parents, to develop durable resistance in lentil cultivars.
Rate of adolescent inpatient admission for psychosis during the COVID‐19 pandemic: A retrospective chart review
Authors: Deren Barbara , Matheson Katherine , Cloutier Paula 
Published: June 2022

AbstractAimGiven the concerns for mental health (MH) impacts on children and adolescents during the COVID‐19 pandemic, as well as the relative paucity of research in this field, this retrospective study compares the rate of paediatric inpatient MH admissions for psychosis for a period of 11 months before and during the pandemic.MethodsWe used administrative data to compare the rate and clinical characteristics of patients (<18 years) admitted to a psychiatric inpatient unit for a psychotic illness before (March 17, 2019 to February 17, 2020) and during (March 17, 2020 to February 17, 2021) the COVID‐19 pandemic.ResultsResults showed a 66% increase in inpatient psychosis admissions from pre‐pandemic rates. More males were admitted with psychosis during the pandemic. Age and length of hospitalization were not significantly different across time periods.ConclusionsResults highlight the importance of accessible MH care for paediatric patients with psychosis during the pandemic.
DNA metabarcoding unravels unknown diversity and distribution patterns of tropical freshwater invertebrates
Authors: Zieritz Alexandra , Lee Ping Shin , Eng Wilhelm Wei Han , Lim Shu Yong , Sing Kong Wah , Chan Wei Ning , Loo Jey Sern , Mahadzir Farah Najwa , Ng Ting Hui , Yeo Darren C.J. , Gan Lydia Xinjie , Gan Jing Ye , Gibbins Christopher , Zoqratt Muhammad Zarul Hanifah Md , Wilson John‐James 
Published: June 2022

Abstract

Tropical freshwater invertebrate species are becoming extinct without being described, and effective conservation is hampered by a lack of taxonomic and distribution data. DNA metabarcoding is a promising tool for rapid biodiversity assessments that has never been applied to tropical freshwater invertebrates across large spatial and taxonomic scales.

Here we use DNA metabarcoding to comprehensively assess the benthic freshwater invertebrate fauna of the Perak River basin, Malaysia. Specific objectives were to: (1) assess performance of two DNA metabarcoding protocols; (2) identify gaps in reference databases; (3) generate new data on species diversity and distribution; and (4) draw conclusions regarding the potential value of DNA metabarcoding in tropical freshwater conservation.

Organisms were collected by hand and net at 34 sites and divided into small (retained in 0.5‐mm but passing through 1‐mm mesh) and large (retained in 1‐mm mesh) fractions, and a 313‐bp cytochrome c oxidase subunit I fragment amplified and sequenced using general Metazoa primers.

Bioinformatic analysis resulted in 468 operational taxonomic units (~species) from 12 phyla. Only 29% of species could be assigned binominal names through matches to public sequence libraries, indicating varying levels of library completeness across Orders. Extraction of small‐fraction DNA with a soil kit resulted in a significantly higher species count than with a general kit, but this was not even across taxa.

Metabarcoding (amplification) success rate, estimated via comparison to morphological identifications of the large‐fraction specimens, was high in most taxa analysed but low, for example, in ampullariid and viviparid gastropods. Conversely, a large proportion of species‐site records for Decapoda and Bivalvia came from metabarcoding only. Species richness averaged 29 ± 16 species per site, dominated by Diptera, Annelida, and Odonata, and was particularly high in tributaries of the mountainous Titiwangsa Range. At least eight species are new records for Malaysia, including the non‐natives Ferrissia fragilis (Gastropoda) and Dugesia notogaea (Platyhelminthes).

Our study showed that DNA metabarcoding is generally more effective in detecting tropical freshwater invertebrate species than traditional morphological approaches, and can efficiently improve knowledge of distribution patterns and ranges of native and non‐native species. However, current gaps in reference databases, particularly for bioindicator taxa, such as the Plecoptera, Ephemeroptera, and Coleoptera, need to be addressed urgently.