bluuebunny/crossref_metadata_2025_split huggingface dataset:
The SUMOylated RREB1 interacts with KDM1A to induce 5‐fluorouracil resistance via upregulating thymidylate synthase and activating DNA damage response pathway in colorectal cancer
Authors: Deng Ya‐Nan , Huang Lan , Gao Shan , Sheng Zenghua , Luo Yinheng , Zhang Nan , Syed Samina Ejaz , Dai Ruiwu , Li Qiu , Fu Xianghui , Liang Shufang 
Published: February 2025

AbstractChemoresistance is one main cause of failure in colorectal cancer (CRC) treatment. The role of transcription factor Ras‐responsive element binding protein 1 (RREB1) remains unclarified in CRC chemoresistance. Herein, we reveal that RREB1 functions as an oncogene to promote cell proliferation and 5‐fluorouracil (5‐FU) chemoresistance in CRC, and SUMOylation is required for RREB1 to exert its oncogenic role in CRC. RREB1 induced cell cycle arrest at the S‐phase and a decreased apoptosis rate under 5‐FU exposure. Mechanistically, the interaction of RREB1 with lysine demethylase 1A (KDM1A) elevated expression of 5‐FU targeting proteins thymidylate synthase (TS) and thymidine kinase (TK1) to maintain the nucleotide pool balance under 5‐FU treatment, and enhanced activation of Chk1‐mediated DNA damage response (DDR) pathway. The deSUMOylation of RREB1 resulted in a reduced interaction of RREB1 with KDM1A, contributing to a downregulation of TS expression and a less activation of DDR pathway. Moreover, KDM1A knockdown improved the DNA damage and reduced RREB1‐mediated resistance to 5‐FU. These findings provide new insights into RREB1‐mediated chemotherapy responses in CRC and indicate RREB1 is a potential target for overcoming 5‐FU resistance.
Qualitative Study of a Dynamical System for Computer Virus Propagation—A Nonstandard Finite‐Difference‐Methodological View
Authors: Wacker Benjamin 
Published: February 2025

ABSTRACTIn this article, we consider a nonlinear model that was originally proposed for computer virus propagation by Gan and coauthors in 2013. As our first contribution, we re‐examined and extended some analytical results regarding this dynamical system. Secondly, we reformulated it by one nonlinear, nonautonomous differential equation. Thirdly, we showed that we can use a classical Newton‐based optimization approach that converges quadratically under mild assumptions for finding the unique equilibrium state of the dynamical system. Fourthly, we proposed a nonstandard finite‐difference‐method based on nonlocal approximations for numerical discretization of our reformulation. Fifthly, we showed that our time‐discrete model possesses the same properties as the time‐continuous model, that is, that namely boundedness and nonnegativity are conserved for arbitrary time‐step sizes. Then we proved convergence towards the time‐continuous solution in the asymptotic sense of letting the time‐step size tend to zero. We finally underlined our theoretical findings by numerical experiments.
3D joint T1/T1ρ/T2 mapping and water‐fat imaging for contrast‐agent free myocardial tissue characterization at 1.5T
Authors: Crabb Michael G. , Kunze Karl P. , Littlewood Simon J. , Tripp Donovan , Fotaki Anastasia , Prieto Claudia , Botnar René M. 
Published: February 2025

AbstractPurposeTo develop a novel, free‐breathing, 3D joint // mapping sequence with Dixon encoding to provide co‐registered 3D , , and maps and water‐fat volumes with isotropic spatial resolution in a single min scan for comprehensive contrast‐agent‐free myocardial tissue characterization and simultaneous evaluation of the whole‐heart anatomy.MethodsAn interleaving sequence over 5 heartbeats is proposed to provide , , and encoding, with 3D data acquired with Dixon gradient‐echo readout and 2D image navigators to enable respiratory scan efficiency. Images were reconstructed with a non‐rigid motion‐corrected, low‐rank patch‐based reconstruction, and maps were generated through dictionary matching. The proposed sequence was compared against conventional 2D techniques in phantoms, 10 healthy subjects, and 1 patient.ResultsThe proposed 3D , , and measurements showed excellent correlation with 2D reference measurements in phantoms. For healthy subjects, the mapping values of septal myocardial tissue were , , and for the proposed sequence, against , , and for 2D MOLLI, 2D ‐prep bSSFP and 2D ‐prep bSSFP, respectively. Promising results were obtained when comparing the proposed mapping to 2D references in 1 patient with active myocarditis.ConclusionThe proposed approach enables simultaneous 3D whole‐heart joint // mapping and water/fat imaging in 7 min scan time, demonstrating good agreement with conventional mapping techniques in phantoms and healthy subjects and promising results in 1 patient with suspected cardiovascular disease.
A Sparsity‐Driven Solution Method for the Cardinality Constrained Mean‐Variance Portfolio Selection Problem
Authors: Jiang Shan , Fang Shu‐Cherng , An Qi 
Published: February 2025

ABSTRACTCardinality constrained mean‐variance (CCMV) portfolio selection problem is commonly formulated as a mixed integer quadratic program (MIQP) that can be solved by a branch‐and‐bound scheme or metaheuristics. Yet, computational efficiency remains to be a major issue. In this study, we propose a novel metaheuristic method based on the concepts of sparse recovery for solving large‐size problems. Using the relationship between investment risk and portfolio sparsity, we develop a “sparsity‐driven” binary search framework for solving the CCMV portfolio selection problem through a sequence of quadratically constrained sparse recovery problems. For computational efficiency, a sub‐one quasi‐norm minimization model is adopted to find high‐quality approximate solutions of each sparse recovery problem using a specially designed gradient‐descent based algorithm. Then, a final solution to the original CCMV portfolio selection problem is obtained by incorporating the approximate solution information to the binary search framework to solve one MIQP problem in a much more reduced size. Computational experiments using the historical stock data from the S&P's Compustat North America Database provided by Wharton Research Database Service clearly indicate the superior performance of the proposed approach in terms of quality of solutions and speed of computation. It runs orders faster than the commonly used CPLEX solver for solving large‐size problems. Comparison with the state‐of‐the‐art perspective reformulation approach in the literature shows that the proposed approach is more efficient for solving large‐size CCMV portfolio selection problems, especially for those without a diagonally dominant covariance matrix. Another experiment with a genetic‐algorithm‐based heuristic method shows that the quality of solutions found by the proposed approach outperforms that of the heuristic method.
Microvascular Coupling in Venous Anastomoses for Head and Neck Reconstruction: A Systematic Review and Meta‐analysis
Authors: Costantino Andrea , Uralov Daniel , Festa Bianca Maria , Pace Gian Marco , Alamoudi Uthman , Haughey Bruce , Iannella Giannicola , Greco Antonio , Magnuson J. Scott , De Virgilio Armando 
Published: February 2025

AbstractObjectiveWe conducted a systematic review and meta‐analysis to compare venous couplers and hand‐sewn techniques for venous anastomosis in head and neck reconstruction.Data SourcesPubMed/MEDLINE and Scopus, databases were searched for relevant publications. Additionally, a manual search was performed in Google Scholar and through reference lists.Review MethodsRetrospective and prospective cohort studies were included. Odds ratios (ORs) and mean differences (MD) were calculated with their 95% confidence intervals (CIs) for each study comparing the 2 groups (coupler vs hand). The inverse variance method was used to combine the effect sizes from the individual studies.ResultsA total of 14,053 patients undergoing 14,270 head and neck free flap reconstructions were included from 52 studies. A total of 6080 flaps were performed using a coupling device for the venous anastomoses, while 8190 flaps were performed with the hand‐sewn technique. No significant difference was found for the venous thrombosis rate (OR: 1.06, 95% CI: 0.65‐1.72), and reoperation rate (OR: 0.93, 95% CI: 0.51‐1.70), but a significantly lower failure rate was measured for the coupler group (OR = 0.34, 95% CI: 0.20‐0.58). A nonsignificant lower operative time was found for venous anastomoses (MD: −20.5, 95% CI: −51.7 to 10.7) and total surgery (MD: −23.7, 95% CI: −344.3 to 296.8) for the coupler group.ConclusionDespite the slight advantages observed with venous couplers, the overall outcomes of both techniques are excellent, and the choice of anastomotic technique should be guided by surgeon preference.
A Targeted Congenital Cytomegalovirus Screening Model Using a Customized Electronic Medical Record Dashboard Report
Authors: Kravitz Meryl B. , Raval Dhvanii K. , Lin Juan , Augustine Stelby , Ramdhanie Mahindra , Oyeku Suzette O. , Nemerofsky Sheri L. , Nassar Michel N. 
Published: February 2025

AbstractObjectiveTargeted congenital Cytomegalovirus (cCMV) screening in newborns has been proposed to identify neonates who could benefit from early diagnosis and intervention. Oftentimes, implementation of targeted screening is variable and left to the discretion of individual institutions. This study investigates the utility of a centralized electronic medical record (EMR) dashboard to improve compliance of targeted screening in the inpatient setting.Study DesignSingle center prospective feasibility studySettingTertiary medical centerMethodsA targeted cCMV screening dashboard report was generated weekly using an EMR system. The dashboard screened all births in a large multi‐site medical center for failed neonatal hearing screens and specific high‐risk diagnoses associated with cCMV. Eligible patients requiring further hearing screening or cCMV testing were communicated to inpatient units. Log‐binomial regression analyses were utilized to assess provider‐led cCMV screening performance after the introduction of weekly reports.ResultsFrom July 1, 2021, to December 31, 2022, 5,866 newborns were screened by the dashboard. Nine‐hundred and eighteen newborns (15.6%) were eligible for testing. Nearly 75% (n = 687) of newborns were tested by inpatient teams prior to report generation. The remaining 25% (n = 231) of eligible newborns were identified by the report but not yet tested by inpatient teams, and subsequently tested. Three of the newborns tested positive for CMV. The rate of eligible newborns appropriately identified by the inpatient teams increased over time (log‐binomial regression coefficient 0.0215, P < .01).ConclusionAn EMR dashboard to guide targeted cCMV screening can improve testing compliance. This model is promising for large‐scale cCMV screening.
Effects of the Central Unit Structure, Lateral Substitution and Symmetry on the Mesomorphic Behavior of Some Bent‐Core Azoester Derivatives
Authors: Ciobanu Catalina‐Ionica , Epure Elena‐Luiza , Soroaga Laurentiu , Simion Aurel , Lisa Gabriela , Carlescu Irina 
Published: February 2025

AbstractWe report here the synthesis and characterization of some new bent‐core asymmetric compounds derived from resorcinol whose thermal behavior has been analyzed by comparison with their analogs derived from 1,3‐disubstituted benzene and 2,7‐dihydroxynaphthalene containing azoester aromatic units and alkyl chain end. The asymmetric structures contain 4‐(4‐alkyloxyphenylazo)‐benzoyl and 4‐methoxy‐benzoyl or 3‐bromo‐4‐methoxy‐benzoyl as side arms. The investigations have been carried out to reach a better understanding of the structure‐properties relationship in such bent‐shaped compounds. We observed that a change in molecular structure like the nature of the central core, the symmetry of the structure or the presence of polar lateral substituent influence not only liquid crystalline properties, but also the thermal behavior. The thermogravimetric analysis showed that the bent‐core derivatives have a good thermal stability since the degradation of the compounds begins over the isotropization temperature. Theoretical calculations were performed to elucidate the behavior of the compounds. These can assist us in designing new molecules that exhibit specific mesomorphic properties.
Compression and in‐plane shear testing of carbon‐epoxy composites at subzero and elevated temperatures: Experiment and modeling
Authors: Nema Aditya , Penumakala Pavan Kumar , Mallineni Chandra Babu , Adusumalli Ramesh , K Tejasvi , Buragohain Manoj Kumar 
Published: February 2025

AbstractMechanical properties of carbon‐epoxy composites are highly affected by the operating temperature. In this study, the effect of temperature on the compressive strength and in‐plane shear strength of composites has been analyzed. The unidirectional composites are processed by filament winding using T700 carbon fiber rovings and two different epoxy resin systems. The compressive strength measured from combined loading compression tests showed a decreasing trend with an increase in temperature. The compressive strength decreased from 700 to 400 MPa when the temperature increased from –20 to 150°C, which represents 54% reduction in strength. The in‐plane shear tests were performed using longitudinal and transverse extensometers and found that shear strength decreased by nearly 50%. Fractography of compression samples revealed that the primary compressive failure mode is micro buckling followed by kinking, at all temperatures. The existing micro mechanical model based on microbuckling and kinking for estimating the compressive strength has been modified to include temperature effects. The key model input parameters are temperature‐dependent resin tensile modulus and in‐plane shear strength. It is found that the modified model will predict the temperature‐dependent compressive strength in the temperature range of −20 to 150°C.Highlights
Compressive strength decreased from 700 MPa at −20°C to 400 MPa at 150°C.
Microbuckling followed by kinking failure was observed at all temperatures.
In‐plane shear strength decreased from 44 MPa at −20°C to 27 MPa at 150°C.
Resin tensile modulus decreased from 2.5 GPa at −20°C to 0.75 GPa at 150°C.
Analytical model for compressive strength was extended for all temperatures.
Development of a Pregnancy Cohort in Commercial Insurance Claims Data: Evaluation of Deliveries Identified From Inpatient Versus Outpatient Claims
Authors: Kahrs Jacob C. , Nickel Katelin B. , Wood Mollie E. , Dublin Sascha , Durkin Michael J. , Osmundson Sarah S. , Stwalley Dustin , Suarez Elizabeth A. , Butler Anne M. 
Published: February 2025

ABSTRACTPurposeStudies using insurance claims data to identify pregnancies are rarely able to directly assess the validity of the pregnancy/delivery. Inpatient versus outpatient delivery claims may provide different levels of evidence, but more stringent requirements could result in exclusion of true pregnancies. We identified delivery codes from the inpatient and outpatient settings and examined possible confirmatory evidence suggesting that a delivery truly occurred.MethodsUsing a US commercial insurance database (2006–2021), we identified potential pregnancies by presence of delivery claims from a provider and/or facility. We classified deliveries as inpatient (claim date during inpatient admission) or outpatient (claim date not during inpatient admission). We identified possible confirmatory evidence for each delivery including: (1) Presence of both provider and facility delivery codes; (2) presence of both diagnosis and procedure delivery codes; (3) labor and delivery revenue codes; (4) gestational age diagnosis codes; (5) pregnancy‐related care codes; (6) linkage to an infant claim; and (7) infant insurance enrollment and linkage to a birthing parent. We quantified the proportion of deliveries with confirmatory evidence by delivery setting. Among deliveries with ≥ 1 piece of confirmatory evidence, we compared patient characteristics by apparent delivery setting.ResultsAmong 4 084 474 delivery episodes, 96.4% were classified as inpatient and 3.6% outpatient. 99.9% of inpatient and 94.0% of outpatient deliveries had ≥ 1 piece of confirmatory evidence. Pregnancy‐related care codes were the most common type of confirmatory evidence (99.0% inpatient, 85.7% outpatient). Deliveries classified as inpatient occurred among patients who were older and more clinically complex (i.e., more pregnancy complications, chronic diseases, and prescription medications).ConclusionsThe vast majority of deliveries had confirmatory evidence regardless of apparent setting. Patient characteristics differed by delivery setting. Inclusion of apparent outpatient deliveries may increase the sample size of the study population and improve the generalizability of study results.
Home‐Based Physiotherapy Programme Reduces Hospital Stay and Costs in Cardiac Surgery. A Retrospective Cohort Study
Authors: Montero‐Cámara Jorge , Ferrer‐Sargues Francisco José , Peredo David Cuesta , Cabello Adrián Sarria , Rovira María José Segrera , Calabuig Juan Antonio Margarit , Valtueña‐Gimeno Noemí , Pardo Juan , Sánchez‐Sánchez María Luz 
Published: February 2025

ABSTRACTBackground and PurposeCardiac surgery enhances cardiovascular disease (CVD) patient survival rates, and the fast‐track protocol can reduce complications, hospital length of stay (HLOS) and associated costs. However, there is no evidence on the effectiveness of unsupervised physiotherapy programmes in reducing HLOS and postoperative pulmonary complications in patients undergoing fast‐track surgery. The study aimed to determine if a pre‐surgical respiratory programme reduces intensive care unit length of stay (ICULOS) and HLOS patients undergoing fast‐track surgery, and its effects on post‐operative complications and healthcare costs.MethodsThis was a retrospective observational cohort study. The patients were divided into two groups: those who followed the exercise programme (pre‐hab group) and those who did not (no pre‐hab group). The study analysed the mean of ICULOS and HLOS for each group, recorded post‐operative complications, and calculated healthcare costs using the Spanish Ministry of Health cost tables.ResultsA total of 418 patients participated in the study with a mean age of 70.44 (10.87) years. The study found significant differences in HLOS (p = 0.001) and ICULOS (p = 0.003) between groups. Pre‐hab HLOS was 232.8 (221.3) hours in the hospital, whereas no pre‐hab LOS increased to 315.1 (277.9) hours. Pre‐hab ICULOS was 82.0 (129.8) hours when No pre‐hab ICULOS accounted 120.2 (190.3) hours. This reduction in hospital admissions resulted in savings of €356,107.16 in average healthcare costs for the pre‐hab group. Mortality risk (p = 0.034) was also reduced in the pre‐hab group (17.7% vs. 26.65% in the no pre‐hab group).DiscussionAn unsupervised home‐based respiratory programme reduces hospital and ICU stay, healthcare costs, post‐surgical pulmonary complications, and mortality risk. The clinical application of an unsupervised home‐based pre‐habilitation programme, focussing on ventilatory exercises and costal expansion techniques, has proven effective in enhancing patient recovery post‐cardiac surgery, offering significant healthcare cost savings, and reducing the burden on hospital resources.
FASTIA: A rapid and accessible platform for protein variant interaction analysis demonstrated with a single‐domain antibody
Authors: Matsunaga Ryo , Tsumoto Kouhei 
Published: February 2025

AbstractAntibodies are critical tools in medicine and research, and their affinity for their target antigens is a key determinant of their efficacy. Traditional antibody affinity maturation and interaction analyses are often hampered by time‐consuming steps such as cloning, expression, purification, and interaction assays. To address this, we have developed FASTIA (Fast Affinity Screening Technology for Interaction Analysis), a novel platform that integrates rapid gene fragment preparation, cell‐free protein synthesis, and bio‐layer interferometry with non‐regenerative analysis. Using this approach, we can analyze the intermolecular interactions of over 20 variants over 2 days, requiring only the parent protein expression plasmid and basic equipment. We have demonstrated the ability of FASTIA to discriminate between single‐domain antibody variants with different binding affinities using the anti‐HEL VHH antibody D2‐L29, and mapped the results to the crystal structure to identify key interaction sites. FASTIA provides results comparable to those obtained using traditional methods. Our system bypasses the need for genetic engineering facilities and can be easily adopted by laboratories, accelerating the protein engineering and optimization processes. In addition, FASTIA is applicable to other protein–protein interactions, making it a versatile tool for studying molecular recognition. FASTIA facilitates efficient affinity maturation, protein engineering, and analysis of protein–protein interactions. This provides a rapid and accessible route for improving antibodies and a broader understanding of protein interactions.
An Unsupervised Approach to Derive Right Ventricular Pressure–Volume Loop Phenotypes in Pulmonary Hypertension
Authors: Sivakumar Nikita , Zhang Cindy , Chang‐Chien Connie , Gu Pan , Li Yikun , Yang Yi , Rosen Darin , Tuhy Tijana , Cubero Salazar Ilton M. , Kauffman Matthew , Damico Rachel L. , Taylor Casey Overby , Greenstein Joseph L. , Hsu Steven , Hassoun Paul M. , Simpson Catherine E. 
Published: February 2025

ABSTRACTAlthough right ventricle (RV) dysfunction drives clinical worsening in pulmonary hypertension (PH), information about RV function has not been well integrated in PH risk assessment. The gold standard for assessing RV function and ventriculo‐arterial coupling is the construction of multi‐beat pressure–volume (PV) loops. PV loops are technically challenging to acquire and not feasible for routine clinical use. Therefore, we aimed to map standard clinically available measurements to emergent PV loop phenotypes. One hundred and one patients with suspected PH underwent right heart catheterization (RHC) with exercise, multi‐beat PV loop measurement, and same‐day cardiac magnetic resonance imaging (CMR). We applied unsupervised k‐means clustering on 10 PV loop metrics to obtain three patient groups with unique RV functional phenotypes and times to clinical worsening. We integrated RHC and CMR measurements to train a random forest classifier that predicts the PV loop patient group with high discrimination (AUC = 0.93). The most informative variable for PV loop phenotype prediction was exercise mean pulmonary arterial pressure (mPAP). Distinct and clinically meaningful PV loop phenotypes exist that can be predicted using clinically accessible hemodynamic and RV‐centric measurements. Exercise mPAP may inform RV pressure–volume relationships.