bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mixed methods evaluation of the acceptability of therapy using LEGO® bricks (LEGO® based therapy) in mainstream primary and secondary education
Authors: Barr Amy , Coates Elizabeth , Kingsley Ellen , de la Cuesta Gina Gomez , Biggs Katie , Le Couteur Ann , Wright Barry 
Published: April 2022

AbstractMany autistic children and young people need extra support with social skills. Social skills programmes, such as LEGO® based therapy (LBT), are commonly used to help with these difficulties. The aim of this study was to examine the acceptability of LBT using qualitative interviews and questionnaires with facilitators and parents/guardians on behalf of autistic children and young people. Acceptability was measured in line with constructs of the Theoretical Framework of Acceptability. Questionnaires were analyzed descriptively and between group comparisons were undertaken using the Mann–Whitney U Test. Telephone interviews were undertaken with a sub‐sample of facilitators. All interviews were recorded, transcribed verbatim, and framework analysis was performed by two researchers supported by NVivo. The questionnaire response rate was 80% for facilitators and 77% for parents/guardians. Overall acceptability, measured on a 1–5 (minimum–maximum) scale, was high for both facilitators and parents/guardians with a median (range) of 5 (4–5) and 4 (3–5), respectively. Facilitators rated the acceptability of the programme significantly higher overall than parents (p < 0.001). Facilitators reported that participants and wider school staff viewed the programme positively. They observed improvements in communication and social skills during the sessions. Potential barriers to programme delivery, such as resources and staff schedules, were identified but facilitators reported that these challenges did not outweigh the benefits. There is increasing emphasis on the role of schools in seeking to improve social outcomes for autistic children therefore this high degree of acceptability makes this an attractive school‐based programme for schools, autistic children and their families.Lay SummarySocial skills programmes, such as LEGO® based therapy (LBT), are often used to help autistic children and young people with their social skills. The acceptability of LBT with school staff and parents/guardians on behalf of children and young people was explored using interviews and questionnaires. Our results show that LBT is viewed as a highly acceptable programme that can help autistic children and young people improve their communication and social skills.
The pro‐apoptotic Bax gene modifies susceptibility to craniofacial dysmorphology following gastrulation‐stage alcohol exposure
Authors: Fish Eric W. , Mendoza‐Romero Haley N. , Love Charlotte A. , Dragicevich Constance J. , Cannizzo Michael D. , Boschen Karen E. , Hepperla Austin , Simon Jeremy M. , Parnell Scott E. 
Published: April 2022

AbstractBackgroundDuring early development, alcohol exposure causes apoptotic cell death in discrete regions of the embryo which are associated with distinctive patterns of later‐life abnormalities. In gastrulation, which occurs during the third week of human pregnancy, alcohol targets the ectoderm, the precursor of the eyes, face, and brain. This midline tissue loss leads to the craniofacial dysmorphologies, such as microphthalmia and a smooth philtrum, which define fetal alcohol syndrome (FAS). An important regulator of alcohol‐induced cell death is the pro‐apoptotic protein Bax. The current study determines if mice lacking the Bax gene are less susceptible to the pathogenic effects of gastrulation‐stage alcohol exposure.MethodsMale and female Bax+/− mice mated to produce embryos with full (−/−) or partial (+/−) Bax deletions, or Bax+/+ wild‐type controls. On Gestational Day 7 (GD 7), embryos received two alcohol (2.9 g/kg, 4 hr apart), or control exposures. A subset of embryos was collected 12 hr later and examined for the presence of apoptotic cell death, while others were examined on GD 17 for the presence of FAS‐like facial features.ResultsFull Bax deletion reduced embryonic apoptotic cell death and the incidence of fetal eye and face malformations, indicating that Bax normally facilitates the development of alcohol‐induced defects. An RNA‐seq analysis of GD 7 Bax+/+ and Bax−/− embryos revealed 63 differentially expressed genes, some of which may interact with the Bax deletion to further protect against apoptosis.ConclusionsOverall, these experiments identify that Bax is a primary teratogenic mechanism of gastrulation‐stage alcohol exposure.
Generation of a host cell line containing a MAR‐rich landing pad for site‐specific integration and expression of transgenes
Authors: Oliviero Claudia , Hinz Steffen C. , Bogen Jan P. , Kornmann Henri , Hock Björn , Kolmar Harald , Hagens Gerrit 
Published: April 2022

AbstractIn recent years, targeted gene integration (TI) has been introduced as a strategy for the generation of recombinant mammalian cell lines for the production of biotherapeutics. Besides reducing the immense heterogeneity within a pool of recombinant transfectants, TI also aims at shortening the duration of the current cell line development process. Here we describe the generation of a host cell line carrying Matrix‐Attachment Region (MAR)‐rich landing pads (LPs), which allow for the simultaneous and site‐specific integration of multiple genes of interest (GOIs). We show that several copies of each chicken lysozyme 5'MAR‐based LP containing either BxB1 wild type or mutated recombination sites, integrated at one random chromosomal locus of the host cell genome. We further demonstrate that these LP‐containing host cell lines can be used for the site‐specific integration of several GOIs and thus, generation of transgene‐expressing stable recombinant clones. Transgene expression was shown by site‐specific integration of heavy and light chain genes coding for a monospecific antibody (msAb) as well as for a bi‐specific antibody (bsAb). The genetic stability of the herein described LP‐based recombinant clones expressing msAb or bsAb was demonstrated by cultivating the recombinant clones and measuring antibody titers over 85 generations. We conclude that the host cell containing multiple copies of MAR‐rich landing pads can be successfully used for stable expression of one or several GOIs.
The triangular fibrocartilage complex in the human wrist: A scoping review toward uniform and clinically relevant terminology
Authors: van der Post Anne‐Sophie , Jens Sjoerd , Daams Joost G. , Obdeijn Miryam C. , Maas Mario , Oostra Roelof‐Jan 
Published: April 2022

AbstractThe aim of this scoping review was to assess the composition, terminology, and anatomy of the triangular fibrocartilage complex (TFCC) of the wrist and propose unambiguous terminology regarding the individual components. The review was conducted according to the methodological framework by Arksey and O'Malley (International Journal of Social Research Methodology, 2005, 8, 19–32). Electronic databases were searched from inception until September 1, 2021 for original anatomical studies, using MeSH terms and keywords on terminology and anatomy of TFCC components. Studies using gross dissections or macro‐ or microscopic histology were included. Animal studies, fetal studies and studies with unknown disease status, were excluded. A total of 24 studies were included. The articular disc, the radioulnar ligaments, the meniscus homologue and the extensor carpi ulnaris tendon (sub)sheath were unanimously classified as TFCC components. One study did not include the ulnolunate and ulnotriquetral ligaments and only one study did include the ligamentum subcruentum. The largest disagreement existed regarding the inclusion of the ulnar collateral ligament. Terminological ambiguity was seen in “triangular fibrocartilage,” “triangular ligament,” “igamentum subcruentum,” and the “proximal and distal lamina.” Anatomical ambiguity existed especially regarding the radioulnar ligaments, the ulnar attachments of the TFCC and the ulnar collateral ligament. Definitions of the individual TFCC components are redundant, ambiguous, and ill‐defined and therefore subject to different interpretations. In order to preclude confusion, consensus regarding terminology is recommended. We proposed a concise definition of the healthy TFCC that can be used as a starting point for future studies and current clinical practice.
A phase I study of talazoparib (BMN 673) combined with carboplatin and paclitaxel in patients with advanced solid tumors (NCI9782)
Authors: Leal Ticiana A. , Sharifi Marina N. , Chan Nancy , Wesolowski Robert , Turk Anita A. , Bruce Justine Y. , O'Regan Ruth M. , Eickhoff Jens , Barroilhet Lisa M. , Malhotra Jyoti , Mehnert Janice , Girda Eugenia , Wiley Elizabeth , Schmitz Natalie , Andrews Shannon , Liu Glenn , Wisinski Kari B. 
Published: April 2022

AbstractBackgroundInhibitors of poly(ADP‐ribose) polymerase (PARP) proteins potentiate antitumor activity of platinum chemotherapy. This study sought to determine the safety and tolerability of PARP inhibitor talazoparib with carboplatin and paclitaxel.MethodsWe conducted a phase I study of talazoparib with carboplatin AUC5‐6 and paclitaxel 80 mg/m2 days 1, 8, 15 of 21‐day cycles in patients with advanced solid tumors. Patients enrolled using a 3 + 3 design in two cohorts with talazoparib for 7 (schedule A) or 3 days (schedule B). After induction with 4–6 cycles of triplet therapy, patients received one of three maintenance options: (a) continuation of triplet (b) carboplatin/talazoparib, or (c) talazoparib monotherapy.ResultsForty‐three patients were treated. The MTD for both schedules was talazoparib 250mcg daily. The main toxicity was myelosuppression including grade 3/4 hematologic treatment‐related adverse events (TRAEs). Dose modification occurred in 87% and 100% of patients for schedules A and B, respectively. Discontinuation due to TRAEs was 13% in schedule A and 10% in B. Ten out of 22 evaluable patients in schedule A and 5/16 patients in schedule B had a complete or partial response. Twelve out of 43 patients received ≥6 cycles of talazoparib after induction, with a 13‐month median duration of maintenance.ConclusionWe have established the recommended phase II dose of Talazoparib at 250mcg on a 3‐ or 7‐day schedule with carboplatin AUC6 and paclitaxel 80 mg/m2 on days 1, 8, 15 of 21‐day cycles. This regimen is associated with significant myelosuppression, and in addition to maximizing supportive care, modification of the chemotherapy component would be a consideration for further development of this combination with the schedules investigated in this study.
Alienated and unsafe: Experiences of the first national UK COVID‐19 lockdown for vulnerable young people (aged 11–24 years) as revealed in Web‐based therapeutic sessions with mental health professionals
Authors: Mindel Charlotte , Salhi Louisa , Oppong Crystal , Lockwood Joanna 
Published: April 2022

AbstractBackgroundThe COVID‐19 pandemic and subsequent lockdowns have disproportionately affected young people, and those who are vulnerable are disadvantaged further. Here, we seek to understand the experiences of vulnerable young people accessing Web‐based therapeutic support during the pandemic and early lockdown, as revealed through the observations of mental health professionals.MethodsFour focus groups with 12 professionals from a digital mental health service were conducted to understand the experiences of vulnerable young people during the pandemic lockdown. Workshops with young people with diverse experiences resulted in the co‐design of the focus group topic guide and the interpretation and validation of analysis. The experiential inductive–deductive framework of thematic analysis was used to analyse the workshop transcripts.ResultsFour main themes and subsequent subthemes were identified: escalation of risk; the experience and consequence of loss; feeling supported and empowered; and feeling separate and isolated.ConclusionsFindings reflect early data that suggest that those with existing vulnerability face an increased risk of poor outcomes through the pandemic and the restrictions of lockdown, but evidence is also provided of positive outcomes from lockdown and its function as a catalyst for change. Results reinforce the need for focused support for vulnerable young people as we emerge from lockdown, and point to supportive and protective factors of relevance to online and offline support provision.
Multivariate temporal process monitoring with graph‐based predictable feature analysis
Authors: Fan Wei , Zhu Qinqin , Ren Shaojun , Xu Bo , Si Fengqi 
Published: April 2022

AbstractDynamic latent variable (DLV) methods have been widely studied for high dimensional time series monitoring by exploiting dynamic relations among process variables. However, explicit extraction of predictable information is rarely emphasized in these DLV methods. In this paper, the graph‐based predictable feature analysis (GPFA) algorithm is introduced for statistical process monitoring due to its explicit predictability, and a novel index, prediction information, is designed to determine the number of its principal components for dimensionality reduction and parameter optimization. A GPFA‐based dynamic process monitoring framework is proposed to differentiate among dynamic faults, normal operating condition changes, and break in relation in the normal data. Case studies on the Tennessee Eastman process and a high‐pressure feedwater heater are conducted to demonstrate the superiority of GPFA over other approaches in terms of fault detection performance.
The Variability of Stable Water Isotopes and the Young Water Fraction in a Mountainous Catchment
Authors: Ye Sheng , Liu Lin , Chen Xiuxiu , Li Peng , Xu Guoce , Ran Qihua 
Published: April 2022

AbstractSoil water movement is an important component in water cycle, which is also closely correlated with the transport of nutrients and contaminants in the catchment. Although there have been many studies in quantifying the water residence in soil, the understanding of the controlling factors is limited and could be controversial. Here, stable isotopes of water to explore the water age distribution in a mountainous catchment in the southwest China are used. The results are consistent with the regional climate pattern that the water vapor of the study catchment is brought by monsoons from Pacific Ocean in summer and prevailing westerly winds in winter. The estimated young water fraction is found to be strongly correlated with topographic gradient but presents no significant correlation with drainage area or vegetation coverage. Comparison between the difference in young water fraction of two adjacent sub‐catchments suggests that soil water in the steeper sub‐catchment tends to favor deep vertical infiltration, resulting in longer flow path, longer mean residence time, and smaller young water fraction. This difference between the soil water movement and the hydrological response revealed in this study has impacts on the biogeochemical cycles in the catchment.
Estrogen receptors observed at extranuclear neuronal sites and in glia in the nucleus accumbens core and shell of the female rat: Evidence for localization to catecholaminergic and GABAergic neurons
Authors: Almey Anne , Milner Teresa A. , Brake Wayne G. 
Published: April 2022

AbstractEstrogens affect dopamine‐dependent diseases/behavior and have rapid effects on dopamine release and receptor availability in the nucleus accumbens (NAc). Low levels of nuclear estrogen receptor (ER) α and ERβ are seen in the NAc, which cannot account for the rapid effects of estrogens in this region. G‐protein coupled ER 1 (GPER1) is observed at low levels in the NAc shell, which also likely does not account for the array of estrogens’ effects in this region. Prior studies demonstrated membrane‐associated ERs in the dorsal striatum; these experiments extend those findings to the NAc core and shell. Single‐ and dual‐immunolabeling electron microscopy determined whether ERα, ERβ, and GPER1 are at extranuclear sites in the NAc core and shell and whether ERα and GPER1 were localized to catecholaminergic or γ‐aminobutyric acid‐ergic (GABAergic) neurons. All three ERs are observed, almost exclusively, at extranuclear sites in the NAc, and similarly distributed in the core and shell. ERα, ERβ, and GPER1 are primarily in axons and axon terminals suggesting that estrogens affect transmission in the NAc via presynaptic mechanisms. About 10% of these receptors are found on glia. A small proportion of ERα and GPER1 are localized to catecholaminergic terminals, suggesting that binding at these ERs alters release of catecholamines, including dopamine. A larger proportion of ERα and GPER1 are localized to GABAergic dendrites and terminals, suggesting that estrogens alter GABAergic transmission to indirectly affect dopamine transmission in the NAc. Thus, the localization of ERs could account for the rapid effects of estrogen in the NAc.
Pharmacokinetics and Bioequivalence of 2 Oral Formulations of Domperidone After Single‐Dose Administration in Healthy Chinese Subjects
Authors: Ye Libing , Shen Minmin , Chen Yingrong , Li Xiaofeng , Xu Min , Yao Chong , Yang Shuixin 
Published: April 2022

AbstractTo evaluate the pharmacokinetic properties and bioequivalence of 2 oral formulations of domperidone in healthy Chinese subjects, a randomized, open‐label, 2‐way crossover study was conducted under fasting and fed states. All 96 healthy subjects were randomized to receive a single oral dose of a 10‐mg generic domperidone tablet (test) or branded domperidone tablet (reference). Blood samples were collected at specified time intervals and analyzed for domperidone using liquid chromatography–tandem mass spectrometry. In the fasting test, 90% CIs of geometric mean ratios were 86.7% to 105.8% for maximum concentration, 96.7% to 106.1% for area under the concentration‐time curve (AUC) from time 0 to the time of the last measurable plasma concentration, and 97.1% to 106.1% for AUC from time 0 extrapolated to infinity. In the fed test, the 90% CIs were 90.8% to 121.1%, 99.7% to 109.4%, and 99.4% to 109.1%, respectively. All 90% CIs were within the bioequivalence range of 80% to 125%, indicating that the 2 formulations were bioequivalent. In addition, the values of time to maximum concentration, terminal‐phase elimination half‐life, and AUC were significantly higher in the fed group than in the fasting group, suggesting that a high‐fat meal slowed down the absorption and elimination of domperidone and significantly increased domperidone exposure.
Effects of irrigation and fertilization on soil salt migration, yield, and water use efficiency of winter wheat in the Yellow River Delta
Authors: Chen Huanyu , Ren Angyan , Hu Zhaohua , Jia Chunqing , Wang Jianlin 
Published: April 2022

AbstractIrrigation and fertilization are the most effective measures to inhibit soil salt accumulation and increase yield of wheat (Triticum aestivum L.) grown in saline soils. The present study used a split‐plot experimental design with irrigation as the main plot and fertilizer application as the subplot from 2014 to 2016. The experiment had four levels each of irrigation (W0, no irrigation; W1, one irrigation; W2, two irrigations; W3, three irrigations) and fertilization (M, no fertilizer; Y, cow manure; W, chemical fertilizer; and H, 50% cow manure and 50% chemical fertilizer). The study identified sowing, jointing, and maturity as the major salt accumulation periods. After irrigation, the 0‐ to 20‐cm soil salinity during presowing, jointing, and grain filling decreased by 1.09, 0.28, 0.19 g kg−1, and 0.76, 0.46, and 0.23 g kg−1, from 2014 to 2016. The wheat roots were mainly distributed in the 0‐ to 30‐cm soil layer. Meanwhile, wheat evapotranspiration (ET) ranged from 153 to 402 and 202 to 451 mm from 2014 to 2016, respectively; and the ET under W3W were 2.62‐ and 2.23‐times higher than W0M. Additionally, W3W increased yield of wheat by 567.0 and 422.2% from 2014 to 2016, compared with W0M. Generally, the water use efficiency (WUE) decreases as irrigation increases; however, this study found no significant difference in WUE between W2 and W3. Meanwhile, the chemical fertilizer resulted in the highest WUE, 3.16‐ and 2.63‐times higher than no fertilizer. Thus, the study proposes W3W as a suitable treatment for wheat in the Yellow River Delta.
Impact of environmental, social and governance disclosure on dividend policy: What is the role of corporate governance? Evidence from an emerging market
Authors: Ellili Nejla Ould Daoud 
Published: April 2022

AbstractThis study examines the nexus between environmental, social, and governance (ESG) disclosure and dividend policy by considering the major role of corporate governance. The sample consists of companies traded in the UAE financial markets during the period 2010–2020. Using data collected from Bloomberg and applying panel data regressions, the results indicated a positive relationship between ESG and dividend payouts. In addition, the results reveal that board of directors' independence is a mediator in the relationship between ESG disclosure and dividend payout, whereas institutional ownership and foreign ownership play a negative and positive moderating role between ESG and dividend payout, respectively. The findings could help UAE financial regulators integrate ESG information into the reporting process, implement effective corporate governance mechanisms to provide accurate information to shareholders, and manage their portfolios more efficiently. This study is the first to examine the relationship between ESG disclosure and dividend policies by considering corporate governance, and contributes to a better understanding of the financial impact of ESG disclosure on dividend policy.
A quantitative method for the simultaneous detection of SR9009 and SR9011 in equine plasma using liquid chromatography–electrospray ionization–tandem mass spectrometry
Authors: Kwak Young Beom , Yu Jundong , Yoo Hye Hyun 
Published: April 2022

AbstractSR9009 and SR9011 are metabolic modulators pharmacologically targeting REV‐ERB receptors as synthetic agonists. A liquid chromatography–tandem mass spectrometry method for the detection of SR9009 and SR9011 in equine plasma was developed and validated. Plasma samples were pretreated by protein precipitation with methanol and were loaded onto an ACQUITY ultra performance liquid chromatography high‐strength silica C18 column (2.1 × 150 mm, 1.8 μm) for chromatographic separation. The mobile phase consisted of 5‐mM ammonium formate (pH 3.0) in distilled water and 0.1% formic acid in acetonitrile, and a gradient elution was used at a flow rate of 0.25 ml/min. For the mass spectrometry detection, the selected reaction monitoring mode was used with transitions of 438.2 → 124.9 for SR9009, 479.2 → 125.1 for SR9011, and 292.2 → 109.1 for the internal standard (testosterone‐d3) in the positive ionization mode. The linearity, lower limit of quantification, intra‐ and inter‐day precision, accuracy, matrix effect, recovery, and stability were evaluated. The method was found to be accurate and reproducible for the quantitation of SR9009 and SR9011. The developed method was successfully applied to plasma samples of thoroughbreds injected intramuscularly with SR9009.