bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short- and Long-Term Biological Variability of Small Dense LDL, HDL3, and Triglyceride-Rich Lipoprotein Cholesterol
Authors: Fatica Erica M , Jenkins Sarah M , Scott Renee J , Block Darci R , Meeusen Jeffrey W , Baumann Nikola A , Saenger Amy K , Donato Leslie J 
Published: May 2022

Abstract

Background
Measurement of cholesterol within lipoprotein subfractions may aid in cardiovascular disease prediction. Simple, homogenous enzymatic assays for the direct measurement of lipoprotein subfractions have been developed to measure small dense low-density lipoprotein cholesterol (sdLDL-C), high-density lipoprotein-3 cholesterol (HDL3-C), and triglyceride-rich lipoprotein (TRL-C) cholesterol. The objective of this study was to determine biological variability for sdLDL-C, HDL3-C, and TRL-C in a healthy reference population to facilitate interpretation of these analytes.

Methods
Serum samples were collected from 24 healthy subjects (n = 14 female/10 male) daily for 3 days while non-fasting, and daily for 5 days, weekly for 4 weeks, and monthly for 6 months after overnight fasting. sdLDL-C, HDL3-C, and TRL-C cholesterol were measured by homogenous enzymatic assays. Sources of variability (between-subject, within-subject, and analytical) were calculated using random-effects regression models. Reference change value (RCV) and index of individuality (II) for each time period were determined from the variance components.

Results
Analytic variability (daily, weekly, and monthly CVA) was &lt;3% for each analyte. Monthly within-subject variability (CVI) was 17.1% for sdLDL-C, 7.4% for HDL3-C, and 25.7% for TRL-C. Most of the monthly variation was attributed to between-subject variation for all 3 analytes. Overall RCVs for monthly measurements were 18.1 mg/dL for sdLDL-C, 6.1 mg/dL for HDL3-C, and 16.0 mg/dL for TRL-C. IIs were &lt;0.6 for sdLDL-C and HDL3-C, and 0.81 for TRL-C.

Conclusions
sdLDL-C, HDL3-C, and TRL-C showed moderate within-subject variability, but high between-subject variability, in a healthy reference population. Given the high individuality of each analyte, population-based reference intervals may be inadequate to detect clinically significant changes.
Molecular Mechanisms of ARID5B-Mediated Genetic Susceptibility to Acute Lymphoblastic Leukemia
Authors: Zhao Xujie , Qian Maoxiang , Goodings Charnise , Zhang Yang , Yang Wenjian , Wang Ping , Xu Beisi , Tian Cheng , Pui Ching-Hon , Hunger Stephen P , Raetz Elizabeth A , Devidas Meenakshi , Relling Mary V , Loh Mignon L , Savic Daniel , Li Chunliang , Yang Jun J 
Published: May 2022

Abstract

Background
There is growing evidence for the inherited basis of susceptibility to childhood acute lymphoblastic leukemia (ALL). Genome-wide association studies have identified non-coding ALL risk variants at the ARID5B gene locus, but their exact functional effects and the molecular mechanism linking ARID5B to B-cell ALL leukemogenesis remain largely unknown.

Methods
We performed targeted sequencing of ARID5B in germline DNA of 5008 children with ALL. Variants were evaluated for association with ALL susceptibility using 3644 patients from the UK10K cohort as non-ALL controls, under an additive model. Cis-regulatory elements in ARID5B were systematically identified using dCas9-KRAB–mediated enhancer interference system enhancer screen in ALL cells. Disruption of transcription factor binding by ARID5B variant was predicted informatically and then confirmed using chromatin immunoprecipitation and coimmunoprecipitation. ARID5B variant association with hematological traits was examined using UK Biobank dataset. All statistical tests were 2-sided.

Results
We identified 54 common variants in ARID5B statistically significantly associated with leukemia risk, all of which were noncoding. Six cis-regulatory elements at the ARID5B locus were discovered using CRISPR-based high-throughput enhancer screening. Strikingly, the top ALL risk variant (rs7090445, P = 5.57 × 10–45) is located precisely within the strongest enhancer element, which is also distally tethered to the ARID5B promoter. The variant allele disrupts the MEF2C binding motif sequence, resulting in reduced MEF2C affinity and decreased local chromosome accessibility. MEF2C influences ARID5B expression in ALL, likely via a transcription factor complex with RUNX1. Using the UK Biobank dataset (n = 349 861), we showed that rs7090445 was also associated with lymphocyte percentage and count in the general population (P = 8.6 × 10–22 and 2.1 × 10–18, respectively).

Conclusions
Our results indicate that ALL risk variants in ARID5B function by modulating cis-regulatory elements at this locus.
Temporal effects of climatic factors on vegetation phenology on the Loess Plateau, China
Authors: Liu Ning , Shi Yufeng , Ding Yongxia , Liu Li , Peng Shouzhang 
Published: May 2022

AbstractStudying the start (SOS) and end (EOS) of the vegetation growing season can improve vegetation prediction under climate change. Climatic factors have temporal effects on vegetation growth, including the no time effect (no), time-lag effect (lag), time-accumulation effect (acc), and both time-lag and -accumulation effects (lagacc). The linear regression equations between SOS/EOS and climatic factors were constructed for the Loess Plateau (LP). Subsequently, we analyzed the effects of single and multiple climatic factors on vegetation phenology under four temporal effect scenarios and investigated the response of vegetation phenology to the time-lag and time-accumulation effects of climatic factors, under the lagacc scenario. Among the four temporal effects, lagacc explained the effects of climate on vegetation phenology to the greatest degree, and it is the optimal temporal effect for simulating the relationship between vegetation phenology and climate on the LP. Moreover, the explanation degrees of multiple climatic factors were higher than those of single climatic factors across different temporal effects and vegetation types. Simultaneously considering multiple climatic factors improved predictability of their impact on vegetation phenology. Under lagacc, responses of SOS to temperature (TMP) and precipitation (PRE) exhibited 1.44 ± 0.43/3.49 ± 0.85 and 1.38 ± 0.30/3.38 ± 0.71 months lag/accumulation, respectively, and responses of EOS to TMP and PRE exhibited 1.35 ± 0.17/2.37 ± 0.34 and 1.59 ± 0.19/4.16 ± 0.50 months lag/accumulation, respectively, across the entire LP. The results show that both lagacc and multiple climatic factors require consideration when establishing relationships between climatic factors and vegetation phenology.
Future of professional work: evidence from legal jobs in Britain and the United States
Authors: Sako Mari , Qian Matthias , Attolini Jacopo 
Published: May 2022

AbstractWhat is the impact of digital technology on professional work? This paper addresses this question by developing a theory on professional jurisdictional control, which we define as a profession’s power to maintain or shift from existing jurisdictional settlements in the face of external disturbances. Digital technology is a disturbance, and who ends up undertaking digital tasks depends on the nature of professional jurisdictional control. With protective jurisdictional control, the profession engages in full or subordinate jurisdiction, delegating new tasks to subordinate semiprofessionals. By contrast, with connective jurisdictional control, the profession prefers settlements by division of labor or advisory links, enabling equal-status professions to work together. Using a large and representative database of online job postings, we find evidence for this hypothesis. Empirically, we deploy three ways to gauge the nature of professional jurisdictional control: first, by comparing traditional law firms and alternative business structure firms in the UK; second, by contrasting the US (with protective jurisdictional control) and the UK; and third, by examining the legal sector (in which the legal profession dominates) and non-legal sectors. We also find that protective (connective) jurisdictional control is associated with lower (higher) pay premia for digital skills, consistent with theory. Our findings highlight the mediating role of professional jurisdictional control to inform the future of work debate.
Minimal invasive resurfacing: an innovative technique for the superior semicircular canal dehiscence. A case series
Authors: Salvinelli Fabrizio , Bonifacio Francesca , Beccaria Claudia , Greco Fabio , Frari Valeria , Iafrati Francesco , Trivelli Maurizio 
Published: May 2022

Abstract
The superior semicircular canal dehiscence is a vestibular disease recognized condition in recent years, and surgical therapy has been modeling itself over the years to ensure the control of vestibular symptoms and auditory symptoms. In this case series, the authors have experienced an intervention aimed at closing the superior semicircular canal dehiscence through the insertion of bone paté between the meninx and the residual middle cranial fossa bone wall. Seven patients underwent this intervention, they reported an improvement in all vestibular and auditory symptoms, and hearing threshold remained stable. Despite the small sample size, the difference was significant in the control of dizziness and the reduction of pulsatile tinnitus. The technique described in this article allows the control of symptoms in superior semicircular canal dehiscence, and it is a type of surgery familiar to the otosurgeon and easily replicable as it involves a modified mastoidectomy. More data are needed.
The evolving properties of the corona of GRS 1915+105: a spectral-timing perspective through variable-Comptonization modelling
Authors: García Federico , Karpouzas Konstantinos , Méndez Mariano , Zhang Liang , Zhang Yuexin , Belloni Tomaso , Altamirano Diego 
Published: May 2022

ABSTRACT
The inverse Compton process by which soft photons are up-scattered by hot electrons in a corona plays a fundamental role in shaping the X-ray spectra of black hole (BH) low-mass X-ray binaries (LMXBs), particularly in the hard and hard-intermediate states. In these states, the power-density spectra of these sources typically show Type-C low-frequency quasi-periodic oscillations (QPOs). Although several models have been proposed to explain the dynamical origin of their frequency, only a few of those models predict the spectral-timing radiative properties of the QPOs. Here, we study the physical and geometrical properties of the corona of the BH-LMXB GRS 1915+105 based on a large sample of observations available in the RXTE archive. We use a recently developed spectral-timing Comptonization model to fit simultaneously the energy-dependent fractional rms amplitude and phase-lag spectra of the Type-C QPO in 398 observations. For this, we include spectral information gathered from fitting a Comptonization model to the corresponding time-averaged spectra. We analyse the dependence of the physical and geometrical properties of the corona upon the QPO frequency and spectral state of the source, the latter characterized by the hardness ratio. We find consistent trends in the evolution of the corona size, temperature, and feedback (the fraction of the corona photons that impinge back on to the disc) that persist for roughly 15 yr. By correlating our observations with simultaneous radio-monitoring of the source at 15 GHz, we propose a scenario in which the disc–corona interactions connect with the launching mechanism of the radio jet in this source.
Fluorodeoxyglucose-positron emission tomography/computed tomography-positive ear lesions responsive to immunosuppressive therapy in a patient with otitis media with antineutrophil cytoplasmic antibody-associated vasculitis
Authors: Murao Yuki , Yoshida Yusuke , Oka Naoya , Yorishima Ai , Masuda Sho , Sugimoto Tomohiro , Ono Rina , Hirokawa Yutaka , Hirata Shintaro 
Published: May 2022

ABSTRACT
A 74-year-old woman presented with vertigo, left-beating nystagmus, and auditory disturbance 4 months prior, in whom a former physician suspected Meniere’s disease. Her signs and symptoms mildly improved with a moderate dose of glucocorticoids, which was eventually tapered. Fluorodeoxyglucose (FDG)-positron emission tomography (PET)/computed tomography (CT) was performed 12 days prior to routine examination. Remarkable FDG uptake was observed in the surrounding areas of the bilateral Eustachian tubes and left middle ear, which was only partially detected on magnetic resonance imaging. The patient also tested positive for myeloperoxidase–antineutrophil cytoplasmic antibody (ANCA). She was admitted to our hospital and diagnosed with otitis media with ANCA-associated vasculitis (OMAAV) based on the classification criteria. Four months after immunosuppressive therapy, the abnormal ear findings were not observed on follow-up FDG-PET/CT. The clinical course of this case suggests that FDG-PET/CT can detect occult ear involvement better than do other modalities in patients with OMAAV. In addition, FDG-PET/CT-positive ear lesions responded to immunosuppressive therapy. Therefore, FDG-PET/CT can help distinguish OMAAV from other ear diseases with non-inflammatory aetiologies and detect occult treatment-responsive OMAAV lesions in the clinical setting.
Successful treatment of bullous lupus with corticosteroids and belimumab: A case report
Authors: Keshavamurthy Chandana , Fibeger Emily , Virata Andrew , Bansal Pankaj 
Published: May 2022

ABSTRACT
Cutaneous lupus erythematosus (CLE) is one of the most common manifestations of systemic lupus erythematosus (SLE), although it can manifest as an independent entity as well. Bullous systemic lupus erythematosus (BSLE) is a rare cutaneous manifestation of SLE presenting as tense vesiculobullous eruptions in a photosensitive distribution. Pathophysiology is secondary to autoantibodies against noncollagenous domain 1 and 2 (NC1 and NC2) type VII collagen, and histopathology reveals dense neutrophilic infiltration of the dermis with direct immunofluorescence showing IgG deposition at dermoepidermal junction. There is lack of data on available therapeutic options to treat BSLE, and varying responses to dapsone, methotrexate, azathioprine and corticosteroids have been reported. Belimumab, a fully humanised Change to Immunoglobulin G1λ (IgG1λ) monoclonal antibody targeting soluble B lymphocyte stimulator protein, was the first Food and Drug Administration-approved drug for SLE and has been reported to be effective for CLE. We present the case of a 41-year-old black female with SLE presenting with BSLE, who was successfully treated with corticosteroids and belimumab and did not experience disease relapse even after discontinuation of corticosteroids. To our knowledge, this is the first reported case of successful treatment of BSLE with belimumab, and further research can help determine the role of belimumab in the treatment of BSLE.
Possible role for rareTRPM7variants in patients with hypomagnesaemia with secondary hypocalcaemia
Authors: Vargas-Poussou Rosa , Claverie-Martin Felix , Prot-Bertoye Caroline , Carotti Valentina , van der Wijst Jenny , Perdomo-Ramirez Ana , Fraga-Rodriguez Gloria M , Hureaux Marguerite , Bos Caro , Latta Femke , Houillier Pascal , Hoenderop Joost G J , de Baaij Jeroen H F 
Published: May 2022

ABSTRACTBackgroundHypomagnesaemia with secondary hypocal-caemia (HSH) is a rare autosomal recessive disorder caused by pathogenic variants in TRPM6, encoding the channel-kinase transient receptor potential melastatin type 6. Patients have very low serum magnesium (Mg2+) levels and suffer from muscle cramps and seizures. Despite genetic testing, a subgroup of HSH patients remains without a diagnosis.MethodsIn this study, two families with an HSH phenotype but negative for TRPM6 pathogenic variants were subjected to whole exome sequencing. Using a complementary combination of biochemical and functional analyses in overexpression systems and patient-derived fibroblasts, the effect of the TRPM7-identified variants on Mg2+ transport was examined.ResultsFor the first time, variants in TRPM7 were identified in two families as a potential cause for hereditary HSH. Patients suffer from seizures and muscle cramps due to magnesium deficiency and episodes of hypocalcaemia. In the first family, a splice site variant caused the incorporation of intron 1 sequences into the TRPM7 messenger RNA and generated a premature stop codon. As a consequence, patient-derived fibroblasts exhibit decreased cell growth. In the second family, a heterozygous missense variant in the pore domain resulted in decreased TRPM7 channel activity.ConclusionsWe establish TRPM7 as a prime candidate gene for autosomal dominant hypomagnesaemia and secondary hypocalcaemia. Screening of unresolved patients with hypocalcaemia and secondary hypocalcaemia may further establish TRPM7 pathogenic variants as a novel Mendelian disorder.
The Role of Diagnostics-Driven Antifungal Stewardship in the Management of Invasive Fungal Infections: A Systematic Literature Review
Authors: Chakrabarti Arunaloke , Mohamed Naglaa , Capparella Maria Rita , Townsend Andy , Sung Anita H , Yura Renee , Muñoz Patricia 
Published: May 2022

Abstract
Antifungal stewardship (AFS) programs are key to optimizing antifungal use and improving outcomes in patients with invasive fungal infections. Our systematic literature review evaluated the impact of diagnostics in AFS programs by assessing performance and clinical measures. Most eligible studies were from Europe and the United States (n = 12/17). Diagnostic approaches included serum β-1–3-D-glucan test (n/N studies, 7/17), galactomannan test (4/17), computed tomography scan (3/17), magnetic resonance (2/17), matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS; 2/17), polymerase chain reaction (1/17), peptide nucleic acid fluorescent in situ hybridization (PNA-FISH) assay (1/17), and other routine methods (9/17). Time to species identification decreased significantly using MALDI-TOF and PNA-FISH (n = 2). Time to targeted therapy and length of empiric therapy also decreased (n = 3). Antifungal consumption decreased by 11.6%–59.0% (7/13). Cost-savings ranged from 13.5% to 50.6% (5/10). Mortality rate (13/16) and length of stay (6/7) also decreased. No negative impact was reported on patient outcomes. Diagnostics-driven interventions can potentially improve AFS measures (antifungal consumption, cost, mortality, and length of stay); therefore, AFS implementation should be encouraged.
MAPKs trigger antiviral immunity by directly phosphorylating a rhabdovirus nucleoprotein in plants and insect vectors
Authors: Ding Zhi-Hang , Gao Qiang , Tong Xin , Xu Wen-Ya , Ma Lulu , Zhang Zhen-Jia , Wang Ying , Wang Xian-Bing 
Published: May 2022

Abstract
Signaling by the evolutionarily conserved mitogen-activated protein kinase or extracellular signal-regulated kinase (MAPK/ERK) plays critical roles in converting extracellular stimuli into immune responses. However, whether MAPK/ERK signaling induces virus immunity by directly phosphorylating viral effectors remains largely unknown. Barley yellow striate mosaic virus (BYSMV) is an economically important plant cytorhabdovirus that is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus) in a propagative manner. Here, we found that the barley (Hordeum vulgare) MAPK MPK3 (HvMPK3) and the planthopper ERK (LsERK) proteins interact with the BYSMV nucleoprotein (N) and directly phosphorylate N protein primarily on serine 290. The overexpression of HvMPK3 inhibited BYSMV infection, whereas barley plants treated with the MAPK pathway inhibitor U0126 displayed greater susceptibility to BYSMV. Moreover, knockdown of LsERK promoted virus infection in SBPHs. A phosphomimetic mutant of the N Ser290 (S290D) completely abolished virus infection because of impaired self-interaction of BYSMV N and formation of unstable N–RNA complexes. Altogether, our results demonstrate that the conserved MAPK and ERK directly phosphorylate the viral nucleoprotein to trigger immunity against cross-kingdom infection of BYSMV in host plants and its insect vectors.
Long-term use of glucocorticoids for polymyalgia rheumatica: follow-up of the PMR Cohort Study
Authors: Muller Sara , Hider Samantha L , Singh Sokhal Balamrit , Lawton Sarah A , Helliwell Toby , Mallen Christian D 
Published: May 2022

Abstract

Objectives
PMR is a common inflammatory condition in older adults, characterized by bilateral hip and shoulder pain and stiffness. Reducing oral glucocorticoids, classically used for ≤2 years, are the mainstay of treatment. This study considers the factors early in the disease course that might be associated with prolonged treatment.

Methods
Six hundred and fifty-two people with incident PMR were recruited from English general practices (2012–2014). Participants completed seven questionnaires over 2 years (used to allocate people to pain–stiffness trajectories) and a further long-term follow-up (LTFU) questionnaire a median of 5.16 years after diagnosis. Characteristics of those still taking and having ceased glucocorticoids were described and compared using Kruskal–Wallis and χ2 and Student’s 2-sample t-tests as appropriate.

Results
Of the 197 people completing the LTFUQ questionnaire, 179 people reported ever having taken glucocorticoids. Of these, 40.1% were still on treatment, with a median (quartile 1, quartile 3) daily dose of 5 (1.5, 9)  mg. People still taking glucocorticoids were more likely to be older (72.5 vs 70.2 years, P = 0.035), live alone (31.8 vs 15.0%, P = 0.01) and have self-managed their glucocorticoid dose (39.1 vs 11.0%, P &lt; 0.0001). They were also more likely to belong to a pain–stiffness trajectory class with sustained symptoms.

Conclusions
PMR is not always a time-limited condition. Few patient characteristics are associated with prolonged treatment early in the disease course, but those who are older and who have sustained symptoms might be at greater risk. Although accurate prediction is not yet possible, clinicians should monitor people with PMR carefully to manage symptoms and reduce the cumulative glucocorticoid dose.
High-fat diet and alcohol induced-mice could cause colonic injury through molecular mechanisms of endogenous toxins
Authors: Zhu Shumin , Huang Haiyang , Xu Shuoxi , Liu Ying , Wu Yayun , Xu Shijie , Huang Song , Gao Jie , He Lian 
Published: May 2022

Abstract
Due to the complexity and diverse causes, the pathological mechanism of diet-induced colonic injury and colitis remains unclear. In this study, we studied the effects of the combination of a high-fat diet (HFD) plus alcohol on colonic injury in mice. We found HFD plus alcohol treatment induced disturbance of the gut microbiota; increased the production of intestinal toxins lipopolysaccharide (LPS), indole, and skatole; destroyed the stability of the intestinal mucosa; and caused the colonic epithelial cells damage through the activation of nuclear factor (NF)-κB and aromatic hydrocarbon receptors (AhR) signaling pathways.
To mimic the effect of HFD plus alcohol in vivo, NCM460 cells were stimulated with alcohol and oleic acid with/without intestinal toxins (LPS, indole, and skatole) in vitro. Combinative treatment of alcohol and oleic acid caused moderate damage on NCM460 cells, while combination with intestinal toxins induced serious cell apoptosis. Western blot data indicated that the activation of NF-κB and AhR pathways further augmented after intestinal toxins treatment in alcohol- and oleic acid-treated colonic cells.
This study provided new evidence for the relationship between diet pattern and colonic inflammation, which might partly reveal the pathological development of diet-induced colon disease and the involvement of intestinal toxins.