bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality Improvement project to study the impact of clinical pharmacist in diabetic management of admitted cardiac patients
Authors: Mekdad Sanaa , Alsayed Leenah 
Published: September 2022

Abstract
Diabetes is a fast-growing chronic disease worldwide. Associated with high morbidity, mortality, and cost. Glycemic control in diabetic patients is a demanding issue and requires partnership with health care professionals. the clinical pharmacist has a positive contribution through a range of extended services to optimize diabetic management and improve patients outcome.
Objectives: This study aims to investigate the role of the clinical pharmacist in the glycemic management of admitted diabetic cardiac patients at King Fahad Medical City.
Method: This is a prospective, cohort study carried out at King Salman Cardiac Center of King Fahad Medical City, cardiac Patients admitted at the cardiac centerfrom October 2018 to October 2020 were randomly assigned to intervention (n= 500) or control group (n=500). Patients were recruited either in the intervention group ( group covered by a clinical pharmacist ) or the control group ( group not covered by a clinical pharmacist ). Follow-up started concurrently with data collection, Glycosylated hemoglobin (HbAC ), fasting blood glucose (FBG),and frequency of hypoglycemic episodes at baseline , and then 6 months after clinical pharmacist intervention
Results:
HbAC levels were reduced in both groups. For the intervention group, HbAC reduction was (8.5 from10.2 , SD= 2.2 ) , (p= 0.04 ). For the control group, HbAC reduction was 9.05 from 9.95 , SD= 1.95 , (p= 0.05) , FBG was decreased in intervention group by 29 mg\dl compared to non-statistical significant by 9 mg\dl decrease in control group . Both the t-test indicated that the HbAC difference was statistically significant.
Conclusions: Clinical pharmacist has a positive impact on monitoring diabetes in admitted cardiac patients.
Immunobiology of a rationally-designed AAV2 capsid following intravitreal delivery in mice
Authors: Whitehead Michael , Sage Andrew , Osborne Andrew , Man Patrick Yu Wai , Martin Keith 
Published: September 2022

Abstract
Adeno-associated virus serotype 2 (AAV2) is a viral vector that can be used to deliver therapeutic genes to diseased cells in the retina. One strategy for altering AAV2 vectors involves the mutation of phosphodegron residues, which are thought to be phosphorylated/ubiquitinated in the cytosol, facilitating degradation of the vector and the inhibition of transduction. As such, mutation of phosphodegron residues have been correlated with increased transduction of target cells, however, an assessment of the immunobiology of wild-type and phosphodegron mutant AAV2 vectors following intravitreal (IVT) delivery to immunocompetent animals is lacking in the current literature. In this study, we show that IVT of a triple phosphodegron mutant AAV2 capsid is associated with higher levels of humoral immune activation, infiltration of CD4 and CD8 T-cells into the retina, generation of splenic germinal centre reactions, activation of conventional dendritic cell subsets, and elevated retinal gliosis compared to wild-type AAV2 capsids. However, we did not detect significant changes in electroretinography arising after vector administration. We also demonstrate that the triple AAV2 mutant capsid is less susceptible to neutralisation by soluble heparan sulphate and anti-AAV2 neutralising antibodies, highlighting a possible utility for the vector in terms of circumventing pre-existing humoral immunity. In summary, the present study highlights novel aspects of rationally-designed vector immunobiology, which may be relevant to their application in preclinical and clinical settings.
Trends in the evolution of Sustainable Development Research in China: A Scientometric Review
Authors: Shi Shaoqing , Yin Jianhua 
Published: September 2022

Abstract
Because of the extensive attention of global scholars on the sustainable development in China, much research has been published over the past 30 years. Based on the 12,635 journal papers from the Web of Science database, we explore the trends in the evolution of China’s sustainable development research by a knowledge graph. The result indicates that the attention of China’s sustainable development research increased exponentially during 1991–2021, and it continues to shift from a macro perspective to the exploration of specific methods and implementation paths. During 2001–2005, China’s sustainable development research developed rapidly and formed a complete cluster structure. In addition, China’s sustainable development research has experienced three stages and two topic drifts. Staged development and topic drifts lead to a wide range of disciplinary drifts. In general, the trends in the evolution of China’s sustainable development research mainly focus on three aspects: research methods, research scope and theoretical innovation. China’s sustainable development provides a case or a path for other developing countries. Economic incentives and policy promotion remain important measures to promote sustainable development.
Bioinformatics-based analysis of key genes in steroid-induced osteonecrosis of the femoral head that are associated with copper metabolism
Authors: Qi Baochuang , Li Chuan , Cai Xingbo , Xu Yongqing , Guo Minzheng , Tang Zhifang , Bo Pengfei , Pu Luqiao 
Published: September 2022

Abstract
Background
The copper metabolism may play a role in angiogenesis and osteogenesis. However, the relationship between copper metabolism and steroid-induced osteonecrosis of the femoral head (SONFH) still remains unclear.
Methods
The GSE123568 dataset and 2062 copper metabolism-related genes (CMRGs) were downloaded from the Gene Expression Omnibus (GEO) and GeneCards databases, respectively. The differentially expressed copper metabolism-related SONFH genes (DE-CMR-SONFHGs) were identified by differential analysis and weighted gene coexpression network analysis (WGCNA). The Least-Absolute Shrinkage and Selection Operator (LASSO) and Support Vector Machine (SVM) algorithms were employed to screen key DE-CMR-SONFHGs. Receiver operating characteristic (ROC) analysis and functional enrichment analysis were performed on the key genes. Targeting drugs were predicted with The Drug Gene Interaction Database (DGIdb). The correlation between key SONFH genes and copper death-related genes was calculated by Pearson method. The result of bioinformatics analysis were confirmed by quantitative real-time polymerase chain reaction (qRT‒PCR).
Results
A total of 106 DE-CMR-SONFHGs were identified. Then, 2 key genes (PNP and SLC2A1) were identified by LASSO and SVM algorithms. The ROC curves of PNP and SLC2A1 indicated that they had diagnostic value in distinguishing SONFH from control samples. Additionally, 20 immune cell populations had notably different abundances between the SONFH and control samples. PNP showed the most significant correlations with plasmacytoid dendritic cells and CD56bright natural killer cells, and SLC2A1 expression showed the most significant correlations with T follicular helper cells and T helper 17 cells. Moreover, 11 drugs that target PNP and 5 drugs that target SLC2A1 were identified. Four copper death-related genes (LIPT1, DLD, PDHB, and MTF1) were upregulated in the disease samples. The qRT‒PCR results showed that PNP and SLC2A1 expression was downregulated and that the copper death-related genes DLD, PDHB and MTF1, which are closely related to these two key genes, were notably highly expressed in the peripheral blood of SONFH patients. These findings were consistent with the bioinformatics analysis results.
Conclusions
Copper homeostatic imbalance may be involved in the process of SONFH. PNP and SLC2A1 were confirmed to be key genes related to SONFH, and these genes may provide insights for the treatment of SONFH.
Potential utility of a multi-component coagulation factor panel to assess the risk of portal vein thrombosis in chronic liver disease
Authors: Lewis Clayton S , Bari Khurram , Xie Changchun , Sherman Kenneth E. , Vasse Marc , Dreden Patrick Van , Bogdanov Vladimir 
Published: September 2022

Abstract
Background: Portal vein thrombosis (PVT) pre-liver transplant (LT) is a major contributor to morbidity in chronic liver disease (CLD); the means of detecting and/or predicting PVT are limited.
Objectives: Explore whether plasma coagulation factor activity levels can help detect PVT and/or serve as a substitute for prothrombin time / international normalized ratio (PT/INR) in the Model for End-stage Liver Disease (MELD).
Methods: Factor V (FV), Factor VIII (FVIII), Protein C (PC), and Protein S (PS) activity levels and the concentrations of D-dimer, sP-selectin, and asTF were assessed in two cohorts of CLD patients (ambulatory, n=42; LT, n=43).
Results: A significant inverse correlation between FVIII activity levels and PVT was found in the LT cohort (p=0.010); FV and PS activity levels were in-trend (p=0.069, p=0.064). We developed a logistic regression-based compensation score to identify patients at risk of PVT. FV and PC activity levels strongly correlated with MELD scores, which enabled the development of a novel scoring system based on multiple linear regressions of the correlations of FV and PC activity with MELD-Na that substitutes PT/INR. 6-month follow-up revealed that our novel formula was non-inferior to MELD-Na at predicting 6-month mortality (c-statistic of 0.627 and 0.615, respectively).
Conclusions: We demonstrate for the first time the potential of using the combination of FV, FVIII, and PS activity levels to assess the risk of PVT in CLD. We also show that FV and PC activity levels may be used to replace PT/INR in MELD scoring.
The Usefulness of Serial Ultrasound in Thyroid Mucosa-Associated Lymphoid Tissue Lymphoma
Authors: Zhang Xiumei , Boxiong Wei , Nong Lin , Zhang Hong , Gao Ying , Ou Jinping 
Published: September 2022

Abstract
Background
Mucosa-associated lymphoid tissue (MALT) lymphoma is an extranodal lymphoma with an indolent natural course. The thyroid gland is an uncommon site of involvement. We aimed to investigate serial ultrasound features during the clinical course of thyroid MALT lymphoma.
Methods
We searched our hospital’s pathology database (5418 patients with thyroid malignancy) between January 2000 and July 2022. The medical records and serial ultrasounds of 11 patients with 12 thyroid MALT lymphoma foci were analyzed retrospectively. from
Results
An enlarging neck mass, dyspnea, B symptoms, and neck lymphadenopathy were seen at diagnosis in 9 (9/11, 81.8%), 3 (3/11, 27.3%), 2 (2/11, 18.2%), and 9 (9/11, 81.8%) cases respectively. Eleven cases were concomitant Hashimoto thyroiditis. Common ultrasound features included bilateral or unilateral asymmetric goiter or large, solid, and very hypoechoic nodules (11/12, 91.7%) interspersed with linear, reticular hyperechoic, and enhanced posterior echoes (11/12, 91.7%), and neck lymph node involvement (10/11, 90.9%). The Thyroid Imaging and Reporting Data System (TIRADS) categories showed higher diagnostic accuracy (11/12, 91.7%) than real-time ultrasound (2/12, 16.7%) in evaluating thyroid lesions for recommendation of fine-needle aspiration (FNA). Serial ultrasound showed self-limiting changes in three cases, relapse in three cases after subtotal thyroidectomy and chemotherapy, large cell transformation (LCT) in one case after left lobectomy, partial remission in one case, and complete remission after chemo/radiation in four cases; progression to enlarged thyroid nodules occurred in three cases without treatment, with no obvious change observed after diagnosis. Three patients died during follow-up.
Conclusion
On sonograms, solid large thyroid nodules or goiter with very hypoechoic and enhanced posterior echoes in the setting of Hashimoto thyroiditis should raise suspicion for MALT lymphoma. TIRADS categories could improve the ultrasound diagnostic efficacy for malignancy. Serial ultrasound examinations demonstrated self-limiting and indolent natures of thyroid MALT lymphoma.
Chimeras in Merlot grapevine revealed by phased assembly
Authors: Sichel Victoria , SARAH Gautier , Girollet Nabil , Laucou Valérie , Roux Catherine , BERT PF , Cunff Loic Le , This Patrice , Lacombe Thierry 
Published: September 2022

Abstract
Chimerism is the phenomenon when several genotypes coexist in a single individual. Used to understand plant ontogenesis they also have been valorised through new cultivar breeding. Viticulture has been taking economic advantage out of chimeras when the variant induced an important modification of wine type such as berry skin colour. Crucial agronomic characters may also be impacted by chimeras that aren’t identified yet. Periclinal chimera where the variant has entirely colonised a cell layer is the most stable and can be propagated through cuttings. In grapevine, leaves have two functional cell layers, L1 and L2. However, lateral roots are formed from the L2 cell layer only. Thus, comparing DNA sequences of roots and leaves allows chimera detection. In this study we used new generation Hifi long reads sequencing, recent bioinformatics tools and trio-binning with parental sequences to detect periclinal chimeras on ‘Merlot’ grapevine cultivar. Sequencing of cv. ‘Magdeleine Noire des Charentes’ and ‘Cabernet Franc’, the parents of cv. ‘Merlot’, allowed haplotype resolved assembly. Pseudomolecules were built with a total of 33 to 47 contigs and in few occasions a unique contig for one chromosome. This high resolution allowed haplotype comparison. Annotation was transferred from PN40024 VCost.v3 to all pseudomolecules. After strong selection of variants, 51 and 53 ‘Merlot’ specific periclinal chimeras were found on the Merlot-haplotype-CF and Merlot-haplotype-MG respectively, 9 and 7 been located in a coding region. These results open new perspectives on chimera detection as an important resource to improve cultivars through clonal selection or breeding.
Water disruption and waterborne communicable diseases in northeast Syria: a spatiotemporal analysis
Authors: Tabor Ruby , Almhawish Naser , Aladhan Ibrahim , Tarnas Maia , Sullivan Richard , Karah Nabil , Zeitoun Mark , Ratnayake Ruwan , Abbara Aula 
Published: September 2022

Abstract

Background
In Syria, disruption to water and sanitation systems, together with poor access to vaccination, forced displacement and overcrowding contribute to increases in waterborne diseases (WBDs). The aim of this study is to perform a spatiotemporal analysis to investigate potential associations between interruptions to water, sanitation and hygiene (WASH) and WBDs in northeast Syria (NES) using data collected by the Early Warning Alert and Response Network (EWARN) from Deir-ez-Zor, Raqqa, Hassakeh and parts of Aleppo governorates.
Methods
We reviewed the literature databases of MEDLINE and Google Scholar and the updates of ReliefWeb to obtain information on acute disruptions and attacks against water infrastructure in NES between January 2015 and June 2021. The EWARN weekly trends of five syndromes representing waterborne diseases were plotted and analysed to identify time trends and the influence of these disruptions. To investigate a potential relationship, the Wilcoxon rank sum test was used to compare districts with and without disruptions. Time series analyses were carried out on major disruptions to analyse their effect on WBD incidence.
Results
The literature review found several instances where water infrastructure was attacked or disrupted, suggesting that water has been deliberately targeted by both state and non-state actors in NES throughout the conflict. Over time, there was an overall upwards trend of other acute diarrhoea (OAD, p < 0.001), but downwards trends for acute jaundice syndrome (AJS), suspected typhoid fever (STF) and acute bloody diarrhoea (ABD). For the major disruption of the Alouk water plant, an interrupted time series analysis did not find a strong correlation between the disruption and changes in disease incidence in the weeks following the incident, but long-term increases in WBD were observed.
Conclusions
While no strong immediate correlation could be established between disruptions to WASH and WBDs in NES, further research is essential to explore the impact of conflict-associated damage to civil infrastructure including WASH. We suggest the implementation of adequate reporting mechanisms for real time tracking of the WASH attacks, damages, direct effects, and likely impact.
The Effects of Online Pilates and Face-To-Face Pilates in Healthy Individuals During the COVID-19 Pandemic: A Randomized Controlled Study
Authors: BULGUROGLU Halil Ibrahim , BULGUROGLU Merve 
Published: September 2022

Abstract
Background: Along with the covid-19 process, people started to turn to online exercise methods. One of these methods is the pilates method, which increases the endurance of the core muscles. This study aims to analyze and compare the effects of online and face-to-face pilates methods.
Methods: Fifty-eight healthy individuals aged 25-40 years were included in the study. Individuals were randomly divided into three groups; online pilates group (OPG), face-to-face pilates group (FPG), and control group (CG). Pilates groups were given pilates exercises in groups of three or four for eight weeks, three days a week, for 1 hour a day, by the physiotherapist. The control group did breathing and relaxation exercises at home. Core muscular endurance, depression, and quality of life were assessed before and after eight weeks of training.
Results: Core muscle endurance, depression, and quality of life improved after pilates in online and face-to-face pilates groups (p<0.05). No change was found in the control group (p > 0.05). When the gains obtained in the pilates groups were compared, it was observed that the improvement in trunk extensor muscle strength was higher in the face-to-face pilates group (p<0.05), and the gains in other parameters were similar (p>0.05).
Conclusions: As a result, healthy individuals have seen similar benefits in online and face-to-face pilates. Both methods are significant for gaining healthy habits and increasing physical activity in healthy individuals.
Trial registration: Retrospectively registered. NCT05309486, Registration date: 04/04/2022.
URL:https://www.clinicaltrials.gov/ct2/show/NCT05309486?term=BULGUROGLU&draw=2&rank=1
Bi-directional association between type 2 diabetes and periodontitis in the nationally representative sample comprising Taiwanese adults from 2000 to 2015
Authors: Chien Wu-Chien , Chung Chi-Hsiang , Fu Earl , Cheng Chia-Mao , Tu Hsiao-Pei , Lee Wei-Cheng , Chen Wei-Liang , Shih Kuang-Chung 
Published: September 2022

Abstract
Objective
The two-way relationship between periodontitis and diabetes was advocated far past. However, the population-based bidirectional observation is still limited. Using the National Health Insurance Research Database of Taiwan, we estimate associations between pre-existing clinical periodontitis and incident type 2 diabetes mellitus (T2DM), and between pre-existing diabetes and incident periodontitis, respectively.
Methods
A total of 11,011 periodontitis patients who underwent periodontal therapy (Pt group) were recruited from 2000 to 2015. After matching by age, sex, and index date, 11,011 periodontitis patients who did not receive treatment (Pwt group) and 11,011 non-periodontitis controls were registered. The outcome of T2DM was traced. Conversely, 157,798 patients with T2DM and 157,798 non-diabetic controls were enrolled, whereas the development of periodontitis was traced. Cox proportional hazards model was performed.
Results
The patients with periodontitis tended to have a statistically high risk of having diabetes. The adjusted hazard ratio (aHR) was 1.94 [95% CI 1.49–2.63, p < 0.01] and 1.72 [95% CI 1.24–2.52, p < 0.01] for Pt and Pwt, respectively. Using Pwt group as a reference, patients in Pt group had a high risk of having diabetes [aHR, 1.17 (95% CI 1.04–1.26, p < 0.001)]. Conversely, patients with T2DM tended to have a high risk of periodontitis [aHR, 1.99 (95% CI 1.44–2.48, p < 0.01)]. However, a high risk was noticed for the outcome subgroup of Pt only [aHR, 2.08 (95% CI, 1.50–2.66), p < 0.001)].
Conclusions
The relationship between periodontitis and diabetes is bi-directional.
Development of a Burst Wave Lithotripsy System for Noninvasive Fragmentation of Ureteroliths in Pet Cats
Authors: Maxwell Adam D. , Kim Ga Won , Furrow Eva , Lulich Jody P. , Torre Marissa , MacConaghy Brian , Lynch Elizabeth , Leotta Daniel F. , Wang Yak-Nam , Borofsky Michael S. , Bailey Michael R. 
Published: September 2022

Abstract
Background: Upper urinary tract stones are increasingly prevalent in pet cats and are difficult to manage. Surgical procedures to address obstructing ureteroliths have short- and long-term complications, and medical therapies (e.g. fluid diuresis and smooth muscle relaxants) are infrequently effective. Burst wave lithotripsy is a non-invasive, ultrasound-guided, handheld focused ultrasound technology to disintegrate urinary stones, which is now undergoing human clinical trials in awake unanesthetized subjects.
Results: In this study, we designed and performed in vitro testing of a modified burst wave lithotripsy system to noninvasively fragment stones in cats. The design accounted for differences in anatomic scale, acoustic window, skin-to-stone depth, and stone size. Prototypes were fabricated and tested in a benchtop model using 35 natural calcium oxalate monohydrate stones from cats. In an initial experiment, burst wave lithotripsy was performed using peak ultrasound pressures of 7.3 (n = 10), 8.0 (n = 5), or 8.9 MPa (n =10) for up to 30 minutes. Fourteen of 25 stones fragmented to <1 mm within the 30 minutes. In a second experiment, burst wave lithotripsy was performed using a second transducer and peak ultrasound pressure of 8.0 MPa (n = 10) for up to 50 minutes. In the second experiment, 9 of 10 stones fragmented to <1 mm within the 50 minutes. Across both experiments, an average of 73-97% of stone mass could be reduced to fragments < 1 mm. A third experiment found negligible injury with in vivo exposure of kidneys and ureters in a porcine animal model.
Conclusions: These data support further evaluation of burst wave lithotripsy as a noninvasive intervention for obstructing ureteroliths in cats.
Robust and Reversible Adhesion Under Extreme Environments
Authors: Chen Jian , Ji Keju , Xu Chi , Zhao Jiahui , Huo Tingwei , Gorb Stanislav N. , Long Yi , Dai Zhendong 
Published: September 2022

Abstract
Adhesive materials have many potential applications for daily life such as soft robotics, wearable devices and transfer printing, however the robust and reversible adhesion, especially under extreme environments including space and polar environment, has rarely been studied and poses an inevitable challenge. We report a new design rule to fabricate an adhesive structure, which possesses the two orders of highest adhesive strength at -100 ℃, excellent reversible adhesion under thermal cycling (from − 100 to 100 ℃) and on ultra-low temperature ice surface (-98.5 ℃). We believe that this new strategy will have wide applications not limited to extreme environments to deploy space exploration and glacier rescue.