bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcatheter valve in valve replacement in stentless prosthetic aortic valves compared to a surgical approach: The lunch is good, but not free!
Authors: Inci Errol , Block Peter C. 
Published: January 2022

Key Points
Particularly in a higher risk population, transcatheter aortic valve in valve replacement is a suitable alternative to repeat surgical valve replacement—with possibly better short term outcomes.
Challenges remain: Valve‐in‐valve TAVR may require a second valve placement and has more residual valve leak than surgery.
Long‐term clinical outcomes comparing transcatheter and surgical replacements for degraded stentless aortic valves are still needed.
Repurposing of 8‐Hydroxyquinoline‐Based Butyrylcholinesterase and Cathepsin B Ligands as Potent Nonpeptidic Deoxyribonuclease I Inhibitors
Authors: Gajic Mihajlo , Knez Damijan , Sosič Izidor , Mravljak Janez , Meden Anže , Košak Urban , Leitzbach Luisa , George Sven , Hofmann Bettina , Zivkovic Aleksandra , Steinhilber Dieter , Stark Holger , Gobec Stanislav , Smelcerovic Andrija , Anderluh Marko 
Published: January 2022

AbstractA library of 31 butyrylcholinesterase (BChE) and cathepsin B (CatB) inhibitors was screened in vitro for inhibition of deoxyribonuclease I (DNase I). Compounds 22, 8 and 7 are among the most potent synthetic non‐peptide DNase I inhibitors reported to date. Three 8‐hydroxyquinoline analogues inhibited both DNase I and BChE with IC50 values below 35 μM and 50 nM, respectively, while two nitroxoline derivatives inhibited DNase I and Cat B endopeptidase activity with IC50 values below 60 and 20 μM. Selected derivatives were screened for various co‐target binding affinities at dopamine D2 and D3, histamine H3 and H4 receptors and inhibition of 5‐lipoxygenase. Compound 8 bound to the H3 receptor and is highlighted as the most promising multifunctional ligand with a favorable pharmacokinetic profile and one of the most potent non‐peptide DNase I inhibitors. The present study demonstrates that 8‐hydroxyquinoline is a structural fragment critical for DNase I inhibition in the presented series of compounds.
Use of Modeling and Simulation to Predict the Influence of Triazole Antifungal Agents on the Pharmacokinetics of Crizotinib
Authors: Chen Lu , Li Lixian , Chen Wanyi 
Published: January 2022

AbstractCrizotinib is used for the treatment of c‐ros oncogene 1–positive advanced non–small‐cell lung cancer. Triazole antifungal agents are widely used for invasive fungal infections in clinical practice. To predict the potential influence of different triazoles (voriconazole, fluconazole, and itraconazole) on the pharmacokinetics of crizotinib by modeling and simulation the physiologically based pharmacokinetic models were established and validated in virtual cancer subjects through Simcyp software based on the essential physicochemical properties and pharmacokinetic data collected. The validated physiologically based pharmacokinetic models were applied to predict the drug‐drug interactions between crizotinib and different triazoles (voriconazole, fluconazole, or itraconazole) in patients with cancer. Crizotinib and triazole antifungal agents were administered orally. The predicted plasma concentration vs time profiles of crizotinib, voriconazole, fluconazole, and itraconazole showed good agreement with observed, respectively. The geometric mean area under the plasma concentration–time curve (AUC) of crizotinib was increased by 84%, 58%, and 79% when coadministered with voriconazole, fluconazole, or itraconazole at multiple doses, respectively. The drug‐drug interaction results showed increased pharmacokinetic exposure (maximum plasma concentration and area under the plasma concentration–time curve) of crizotinib when coadministrated with different triazoles (voriconazole > itraconazole > fluconazole). Among the 3 triazoles, voriconazole exhibited the most significant influence on the pharmacokinetic exposure of crizotinib. In clinic, adverse drug reactions and toxicity related to crizotinib should be carefully monitored, and therapeutic drug monitoring for crizotinib is recommended to guide dosing and optimize treatment when coadministered with voriconazole, fluconazole, or itraconazole.
Schooling practices, curriculum, and educational opportunities of the children in the fisherman' neighbourhoods in Indonesia archipelago
Authors: Aletheiani Dinny Risri 
Published: January 2022

AbstractThis paper focuses on the schooling practices, curriculum, and educational opportunities available to children in the fishermen's neighbourhoods of the Indonesian archipelago. The study investigates what schooling practices, curriculum, and educational opportunities are thought important for the education of children in fishermen's neighbourhoods of the Indonesian archipelago. The study utilises archival and policy texts, created teaching and learning materials from the Indonesian Ministry of Education and Culture, and various texts derived from the community‐based foundations and non‐profit organisations spread out in the Indonesian archipelago related to the education of children in Indonesian fishermen's neighbourhoods. Through an archival investigation of curricula, the study hopes to examine the languages of instrumentality and criticality and how they have shaped the educational practices and experiences in these fishermen's neighbourhood contexts.
Direct sequence confirmation of qPCR products for gene doping assay validation in horses
Authors: Maniego Jillian , Pesko Bogusia , Hincks Pamela , Taylor Polly , Stewart Graham , Proudman Christopher , Scarth James , Ryder Edward 
Published: January 2022

AbstractThe misuse of gene therapy by the introduction of transgenes via plasmid or viral vectors as a doping agent is an increasing concern in human and animal sports, not only in consideration to fair competition but also in potential detrimental effects to welfare. Doping events can be detected by polymerase chain reaction (PCR) amplification of a transgene‐specific region of DNA. Quantitative real‐time PCR (qPCR) is particularly suited to confirmatory investigations where precise limits of detection can be calculated. To fully validate a qPCR experiment, it is highly desirable to confirm the identity of the amplicon. Although post‐PCR techniques such as melt curve and fragment size analysis can provide strong evidence that the amplicon is as expected, sequence identity confirmation may be beneficial as part of regulatory proceedings. We present here our investigation into two alternative processes for the direct assessment of qPCR products for five genes using next‐generation sequencing: ligation of sequence‐ready adapters to qPCR products and qPCR assays performed with primers tailed with Illumina flow cell binding sites. To fully test the robustness of the techniques at concentrations required for gene doping detection, we also calculated a putative limit of detection for the assays. Both ligated adapters and tailed primers were successful in producing sequence data for the qPCR products without further amplification. Ligated adapters are preferred, however, as they do not require re‐optimisation of existing qPCR assays.
Autophony in inpatients with anorexia nervosa or avoidant restrictive food intake disorder
Authors: Hollis Jeff , Mann Scott , Watters Ashlie , Oakes Judy , Mehler Philip S. 
Published: January 2022

AbstractObjectiveTo assess for the prevalence of autophony, a distressing auditory symptom commonly attributed to patulous eustachian tube, in a cohort of individuals with severe malnourishment due to an eating disorder.MethodA cross‐sectional survey study was performed. Patients admitted for inpatient medical stabilization of an eating disorder, who were also at low body weight, were asked to complete a survey assessing aural symptoms present in the previous 24 hr, including autophony. Anthropometric data and prealbumin levels were collected.ResultsOf 101 patients enrolled, 43 (42.6%) reported symptoms of autophony. The presence of autophony was associated with lower serum prealbumin levels and lower body weight as measured by percentage of ideal body weight.DiscussionAutophony is a commonly reported, albeit rarely discussed, symptom in individuals with severe eating disorders and correlates with degree of malnutrition.
A comprehensive study of different objectives and solutions of controller placement problem in software‐defined networks
Authors: Dhar Mili , Debnath Arindam , Bhattacharyya Bidyut K. , Debbarma Mrinal Kanti , Debbarma Swapan 
Published: January 2022

AbstractSoftware‐defined network (SDN) is a programmable networking paradigm that enables logically centralized network management. In order to overcome the shortcomings of traditional networks, SDN has come into the picture. SDN is a recent and fast‐forwarding technique that involves separating the network's control plane from the data plane. This network also allows us to directly communicate with the applications through application programming interfaces. A network administrator can directly modify and update the routing policies and rules in SDN. Apart from the advantages given by the SDN, it has also been found that separation of control plane directly affects the network performance. Finding the optimum number of controllers and their locations to improve the performance is one of the fundamental research problems in SDN which is also commonly known as the controller placement problem (CPP). In this article, we have addressed the CPP in SDN in detail and investigated the optimization objectives that influence CPP. The state‐of‐the‐art of this article is to review almost all the optimization objective along with their mathematical formulation and survey the CPP solutions proposed by the other researchers'. Classified the CPP into three parts based on the objectives and solutions of CPP and reviewed the impact of these objectives on network performance. This article gives a comprehensive overview of current strategies on CPP in SDN and at last, we give some research issues that can be further explored by the researchers.
Wave propagation in inhomogeneous media via FE/PML method
Authors: Savidis Stavros , Bergmann Mathias , Schepers Winfried , Fontara Ioanna‐Kleoniki 
Published: January 2022

AbstractThe Perfectly Matched Layer (PML) method is an efficient approach to imposing radiation conditions at the bounded region of interest in case of wave propagation in unbounded domains. This paper presents and validates 3D FE/PML numerical schemes based on two different PML formulations for homogeneous and inhomogeneous geological media exhibiting discrete or continuous inhomogeneity. In the equation of motion for the PML domain the applied stretching behavior is expressed either as complex material properties or as complex coordinates. Both PML formulations are implemented in the FEM and verified against analytical solutions. Three different types of material inhomogeneity are considered: layered half‐space, continuously inhomogeneous half‐space with linear velocity profile and continuously inhomogeneous half‐space with nonlinear velocity profile. Sensitivity analyses are conducted, and the performance of the developed numerical schemes is investigated taking into account a broad variation of the PML parameters. Recommendations are given for the optimal values of the PML parameters for the case of homogeneous and inhomogeneous geological media.
The productive efficiency of community health service stations in China: Taking Shandong province as a case
Authors: Qiu Leijie , Yang Linsheng , Li Hairong , Wang Li 
Published: January 2022

AbstractThe present study evaluated the efficiencies of community health service (CHS) stations based on a survey of 1246 CHS stations, covering nine subcategories in 16 cities in Shandong province. Data envelopment analysis (DEA) was applied to investigate the overall efficiency, the technical and the scale efficiency of community health care resources. The results are, the overall efficiency was 9.47%, and the overall efficiency was generally higher in the central‐west region than in the east. There were 23.27% of CHS stations showing technically efficient. The technical efficiency was higher in the east (31.11%) relative to the central‐west (19.72%), and 72.71% of CHS stations had a technical efficiency higher than the regional average efficiency. The scale efficiency was 9.31% for CHS stations in Shandong province, being the decisive factor for overall efficiency, and 68.96% of CHS stations showed a scale efficiency above the regional average. Stations held by enterprises and universities, and extended by tertiary hospitals had lower efficiencies than other types of CHS stations. In conclusion, the CHS stations had low efficiencies in general, and scale inefficiencies were the main cause. Related suggestions to improve the efficiency are provided accordingly.
Disparities in demand for COVID‐19 hospital care in the United States: Insights from a longitudinal hierarchical study
Authors: Messner Wolfgang 
Published: January 2022

AbstractBackground and aimsThis study examined disparities in hospitalization for COVID‐19 within the U.S. by racial and ethnic groups, health insurance status, and social support structure.MethodsUsing publicly available ecological case and contextual data from July 2020 to April 2021, a longitudinal hierarchical model for the 51 U.S. states was constructed.ResultsRacial/ethnic disparities were observed, such as that hospitalization rates were higher in states with a higher percentage of Black (β = .002, P = .009) and American Indian or Alaska Native persons (β = .003, P = .03). Conversely, lack of health insurance was related to a lower hospitalization rate (β = −.005, P = .002), and so was a stronger social support system (β = −.015, P = .05).ConclusionThese differences suggest disparities in COVID‐19 incidence, symptom severity, and demand for hospital care. Understanding how they contribute to geographic differences in hospitalization can help guide public health decisions and resource allocation to address COVID‐19‐related health inequalities.
Optimizing large‐scale autologous human keratinocyte sheets for major burns—Toward an animal‐free production and a more accessible clinical application
Authors: Frese Laura , Darwiche Salim Elias , Gunning Myrna Elisabeth , Hoerstrup Simon Philipp , von Rechenberg Brigitte , Giovanoli Pietro , Calcagni Maurizio 
Published: January 2022

AbstractBackground and AimsAutologous keratinocyte sheets constitute an important component of the burn wound treatment toolbox available to a surgeon and can be considered a life‐saving procedure for patients with severe burns over 50% of their total body surface area. Large‐scale keratinocyte sheet cultivation still fundamentally relies on the use of animal components such as inactivated murine 3T3 fibroblasts as feeders, animal‐derived enzymes such as trypsin, as well as media components such as fetal bovine serum (FBS). This study was therefore aimed to optimize autologous keratinocyte sheets by comparing various alternatives to critical components in their production.MethodsHuman skin samples were retrieved from remnant operative tissues. Cell isolation efficiency and viability were investigated by comparing the efficacy of porcine‐derived trypsin and animal‐free enzymes (Accutase and TrypLESelect). The subsequent expansion of the cells and the keratinocyte sheet formation was analyzed, comparing various cell culture substrates (inactivated murine 3T3 fibroblasts, inactivated human fibroblasts, Collagen I or plain tissue culture plastic), as well as media containing serum or chemically defined animal‐free media.ResultsThe cell isolation step showed clear cell yield advantages when using porcine‐derived trypsin, compared to animal‐free alternatives. The keratinocyte sheets produced using animal‐free serum were similar to those produced using 3T3 feeder layer and FBS‐containing medium, particularly in mechanical integrity as all grafts were liftable. In addition, sheets grown on collagen in an animal‐free medium showed indications of advantages in homogeneity, speed, reduced variability, and differentiation status compared to the other growth conditions investigated. Most importantly, the procedure was compatible with the up‐scaling requirements of major burn wound treatments.ConclusionThis study demonstrated that animal‐free components could be used successfully to reduce the risk profile of large‐scale autologous keratinocyte sheet production, and thereby increase clinical accessibility.
Trial of labor after cesarean in twin gestation with no prior vaginal delivery – evidence from largest cohort reported
Authors: Levin Gabriel , Yagel Simcha , Schwartz Anat , Many Ariel , Rosenbloom Joshua I. , Yinon Yoav , Meyer Raanan 
Published: January 2022

AbstractObjectiveTo investigate trial of labor after cesarean (TOLAC) success rates in twin gestations with no prior vaginal delivery.MethodsA retrospective study of women with twin gestations who underwent a TOLAC and had no prior vaginal delivery during 2011–2020. TOLAC success and failure groups were compared.ResultsOf 675 twin gestations with a history of cesarean delivery and no prior vaginal delivery, 83 (12.3%) elected to undergo a TOLAC and 26 (31.3%) succeeded. Two (7.7%) women delivered by cesarean for the second twin after vaginal delivery of the first twin. Epidural analgesia was positively associated with TOLAC success (odds ratio [OR] 4.31, 95% confidence interval [CI] 1.56–11.94, P = 0.004). Uterine rupture occurred in two patients (3.5%) of the TOLAC failure group. The proportion of cases with low Apgar score (<7) at 5 min was higher in the TOLAC success group (4 [15.4%] versus 1 [1.8%]; OR 10.1, 95% CI 1.07–96.22, P = 0.032) and the neonatal composite adverse outcome rate was lower in this group (OR 0.22, 95% CI 0.07–0.69, P = 0.009).ConclusionTOLAC in women with twins with no prior vaginal delivery is associated with a low success rate. No independent predictors of successful TOLAC were identified.
Acute exposure to abuse‐like concentrations of toluene induces inflammation in mouse lungs and brain
Authors: Svenson Drew W. , Davidson Cameron J. , Thakur Chitra , Bowen Scott E. 
Published: January 2022

AbstractToluene is an aromatic hydrocarbon commonly abused by young adolescents for its central nervous system depressant effects. Although toluene's pharmacological effects at high concentrations are relatively well‐known, few studies have assessed toluene's effects on lung and brain tissues. The present study characterized the pathological effects of acute inhaled toluene exposure in the lungs and brains of male Swiss‐Webster mice (N = 68). Using a static vapor exposure chamber, mice (PND 28) received a single 30‐min toluene administration (0, 1000, 2000, or 4000 ppm). Lung and brain tissues were extracted 24‐h post‐exposure. Histology results revealed significant changes in the morphology of lung tissue (e.g., irregular cellular architecture) with the 2000‐ and 4000‐ppm exposures expressing greater signs of pathology than control 0‐ppm exposure. Markers of immune system activity (F4/80 and Ly‐6G) and cellular proliferation (Ki‐67) in the lung revealed no significant differences. Additionally, brain tissues were analyzed for changes of astrogliosis (glial fibrillary acidic protein [GFAP]) and oxidative stress (glutathione peroxidase [GPx]). GFAP showed increased astrogliosis in the striatum with 2000‐ppm toluene showing significantly higher expression than control (p < 0.05) and a marginal effect in the hippocampus. No other markers showed significant changes. The increased signs of inflammation and cellular damage suggest that exposure to a single high concentration of toluene, typical of abuse, is capable of producing pathology in both lung and brain tissue.
Featuring few essential Raman spectroscopic signatures between heterogeneous cells
Authors: Dai Yixin , Wang Liu , Luo Chuan , Li Wenkang , Huang Qing , Li Wenxue , Pang Lin 
Published: January 2022

AbstractHere we demonstrate it is instructive to quantify cell Raman spectroscopy by sparse regularization. To be able to extract the specific spectral differences in a heterogeneous cell system with great spectroscopic similarities derived from many common biomolecular components, the maximum information entropy probability was proposed and exemplified by identifying normal lymphocytes from leukemia cells. The essential spectroscopic features were observed to locate at three Raman peaks whose spectral signatures were commensurate. The applicability of the extracted features was acknowledged by that the predicted identification accuracy of up to 93% was still achieved when only two peaks were loaded into decision tree model, which may provide the possibility of a clinically rapid hematological malignancy detection.
Social justice outcomes and professional counseling: An 11‐year content analysis
Authors: Clark Madeline , Moe Jeffry , Chan Christian D. , Best Mihkaya D. , Mallow Laura M. 
Published: January 2022

AbstractArticles that assessed the outcomes of interventions based on multicultural‐social justice principles, and that were published in a journal sponsored by the American Counseling Association or its divisions, Chi Sigma Iota, or the National Board for Certified Counselors, were analyzed using qualitative content analysis. A total ofN= 35 empirical studies were identified for inclusion. The year published, methodology, population studied, sample size, operationalized constructs, and whether the study espoused a systemic or community focus were coded. Principal findings indicated a slight increase in multicultural‐social justice outcome research over time, a need for more rigorous multicultural‐social justice outcome research, and a need for scholars and practitioners to enhance their systemic and community‐based conceptualizations of their clients’ presenting concerns. Other implications for counseling, limitations, and future directions are discussed.
Circulating metabolites serve as diagnostic biomarkers for HER2‐positive breast cancer and have predictive value for trastuzumab therapy outcomes
Authors: Mao Changfei , Wang Min , Li Li , Tang Jin‐Hai 
Published: January 2022

AbstractHuman epidermal growth factor receptor 2 (HER2)‐positive is a particularly aggressive type of the breast cancer. Trastuzumab‐based therapy is a standard treatment for HER2‐positive breast cancer, but some patients are resistance to the therapy. There are no known diagnostic biomarkers to improve the early diagnosis of HER2‐positive breast cancer and the clinical utility of trastuzumab therapy. Using ultrahigh‐performance liquid time of flight mass spectrometry (UPLC‐TOF‐MS)‐based serum metabolomics and multivariate statistical analysis, we investigated and identified the circulating metabolites L‐arginine and arachidonic acid were elevated in trastuzumab‐responsive and trastuzumab‐resistant HER2‐positive breast cancer patients, and increased until reaching their peaks in trastuzumab‐resistant HER2‐positive breast cancer patients. Moreover, an equation for assessing the risk scores based on linear logistic regression models involving L‐arginine and arachidonic acid was created, which was beneficial for revealing metabolic changes in HER2‐positive breast cancer and enhancing current trastuzumab‐based therapy. In summary, we develop serum‐based metabolic biomarkers for diagnosis of HER2‐positive breast cancers and predicts the therapeutic effects of trastuzumab therapy.