bluuebunny/crossref_metadata_2025_split huggingface dataset:
Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries
Authors: Sharrock Thomas E. , Evans Jenny , Blanchard Guy B. , Sanson Bénédicte 
Published: September 2022

ABSTRACT
Compartmental boundaries physically separate developing tissues into distinct regions, which is fundamental for the organisation of the body plan in both insects and vertebrates. In many examples, this physical segregation is caused by a regulated increase in contractility of the actomyosin cortex at boundary cell-cell interfaces, a property important in developmental morphogenesis beyond compartmental boundary formation. We performed an unbiased screening approach to identify cell surface receptors required for actomyosin enrichment and polarisation at parasegmental boundaries (PSBs) in early Drosophila embryos, from the start of germband extension at gastrulation and throughout the germband extended stages (stages 6 to 11). First, we find that Tartan is required during germband extension for actomyosin enrichment at PSBs, confirming an earlier report. Next, by following in real time the dynamics of loss of boundary straightness in tartan mutant embryos compared with wild-type and ftz mutant embryos, we show that Tartan is required during germband extension but not beyond. We identify candidate genes that could take over from Tartan at PSBs and confirm that at germband extended stages, actomyosin enrichment at PSBs requires Wingless signalling.
The emerging significance of splicing in vertebrate development
Authors: Olthof Anouk M. , White Alisa K. , Kanadia Rahul N. 
Published: September 2022

ABSTRACT
Splicing is a crucial regulatory node of gene expression that has been leveraged to expand the proteome from a limited number of genes. Indeed, the vast increase in intron number that accompanied vertebrate emergence might have aided the evolution of developmental and organismal complexity. Here, we review how animal models for core spliceosome components have provided insights into the role of splicing in vertebrate development, with a specific focus on neuronal, neural crest and skeletal development. To this end, we also discuss relevant spliceosomopathies, which are developmental disorders linked to mutations in spliceosome subunits. Finally, we discuss potential mechanisms that could underlie the tissue-specific phenotypes often observed upon spliceosome inhibition and identify gaps in our knowledge that, we hope, will inspire further research.
The single Marchantia polymorpha FERONIA homolog reveals an ancestral role in regulating cellular expansion and integrity
Authors: Mecchia Martin A. , Rövekamp Moritz , Giraldo-Fonseca Alejandro , Meier Dario , Gadient Philippe , Vogler Hannes , Limacher Daria , Bowman John L. , Grossniklaus Ueli 
Published: September 2022

ABSTRACT
Plant cells are surrounded by a cell wall, a rigid structure that is not only important for cell and organ shape, but is also crucial for intercellular communication and interactions with the environment. In the flowering plant Arabidopsis thaliana, the 17 members of the Catharanthus roseus RLK1-like (CrRLK1L) receptor kinase family are involved in a multitude of physiological and developmental processes, making it difficult to assess their primary or ancestral function. To reduce genetic complexity, we characterized the single CrRLK1L gene of Marchantia polymorpha, MpFERONIA (MpFER). Plants with reduced MpFER levels show defects in vegetative development, i.e. rhizoid formation and cell expansion, and have reduced male fertility. In contrast, cell integrity and morphogenesis of the gametophyte are severely affected in Mpfer null mutants and MpFER overexpression lines. Thus, we conclude that the CrRLK1L gene family originated from a single gene with an ancestral function in cell expansion and the maintenance of cellular integrity. During land plant evolution, this ancestral gene diversified to fulfill a multitude of specialized physiological and developmental roles in the formation of both gametophytic and sporophytic structures essential to the life cycle of flowering plants.
DeepProjection: specific and robust projection of curved 2D tissue sheets from 3D microscopy using deep learning
Authors: Haertter Daniel , Wang Xiaolei , Fogerson Stephanie M. , Ramkumar Nitya , Crawford Janice M. , Poss Kenneth D. , Di Talia Stefano , Kiehart Daniel P. , Schmidt Christoph F. 
Published: September 2022

ABSTRACT
The efficient extraction of image data from curved tissue sheets embedded in volumetric imaging data remains a serious and unsolved problem in quantitative studies of embryogenesis. Here, we present DeepProjection (DP), a trainable projection algorithm based on deep learning. This algorithm is trained on user-generated training data to locally classify 3D stack content, and to rapidly and robustly predict binary masks containing the target content, e.g. tissue boundaries, while masking highly fluorescent out-of-plane artifacts. A projection of the masked 3D stack then yields background-free 2D images with undistorted fluorescence intensity values. The binary masks can further be applied to other fluorescent channels or to extract local tissue curvature. DP is designed as a first processing step than can be followed, for example, by segmentation to track cell fate. We apply DP to follow the dynamic movements of 2D-tissue sheets during dorsal closure in Drosophila embryos and of the periderm layer in the elongating Danio embryo. DeepProjection is available as a fully documented Python package.
Sea anemone Frizzled receptors play partially redundant roles in oral-aboral axis patterning
Authors: Niedermoser Isabell , Lebedeva Tatiana , Genikhovich Grigory 
Published: September 2022

ABSTRACT
Canonical Wnt (cWnt) signalling is involved in a plethora of basic developmental processes such as endomesoderm specification, gastrulation and patterning the main body axis. To activate the signal, Wnt ligands form complexes with LRP5/6 and Frizzled receptors, which leads to nuclear translocation of β-catenin and a transcriptional response. In Bilateria, the expression of different Frizzled genes is often partially overlapping, and their functions are known to be redundant in several developmental contexts. Here, we demonstrate that all four Frizzled receptors take part in the cWnt-mediated oral-aboral axis patterning in the cnidarian Nematostella vectensis but show partially redundant functions. However, we do not see evidence for their involvement in the specification of the endoderm – an earlier event likely relying on maternal intracellular β-catenin signalling components. Finally, we demonstrate that the main Wnt ligands crucial for the early oral-aboral patterning are Wnt1, Wnt3 and Wnt4. Comparison of our data with knowledge from other models suggests that distinct but overlapping expression domains and partial functional redundancy of cnidarian and bilaterian Frizzled genes may represent a shared ancestral trait.
The ETS transcription factor Spi2 regulates hematopoietic cell development in zebrafish
Authors: Zhao Shizheng , Zhang Ao , Zhu Hao , Wen Zilong 
Published: September 2022

ABSTRACT
The E26 transformation-specific or E-twenty-six (ETS) genes encode a superfamily of transcription factors involved in diverse biological processes. Here, we report the identification and characterization of a previously unidentified member of the ETS transcription factors, Spi2, that is found exclusively in the ray-finned fish kingdom. We show that the expression of spi2 is restricted to hemogenic endothelial cells (HECs) and to hematopoietic stem and progenitor cells (HSPCs) in zebrafish. Using bacteria artificial chromosome transgenesis, we generate a spi2 reporter line, TgBAC(spi2:P2a-GFP), which manifests the GFP pattern recapitulating the endogenous spi2 expression. Genetic ablation of spi2 has little effect on HEC formation and the endothelial-to-hematopoietic transition, but results in compromised proliferation of HSPCs in the caudal hematopoietic tissue (CHT) during early development and in severe myeloid lineage defect in adulthood. Epistatic analysis shows that spi2 acts downstream of runx1 in regulating HSPC development in the CHT. Our study identifies Spi2 as an essential regulator for definitive hematopoietic cell development and creates a TgBAC(spi2:P2a-GFP) reporter line for tracking HECs, HSPCs, myeloid cells and thrombocytes from early development to adulthood.
The role of lipid peroxidation in the pathogenesis of experimental diabetic retinopathy
Authors: Sirman Y. , Savytskyi I. 
Published: September 2022

The aim of work – to analysis of changes in the level of primary products of lipid peroxidation in experimental animals were simulated diabetic retinopathy and against the background of its correction.
The obtained results indicate an increase in the content of lipid peroxidation, starting from the 30th and with further progression on the 60th and 180th days of experimental diabetic retinopathy, which is confirmed by an increase in the level of diene conjugates in the 2nd group, the maximum of which is observed at the 3rd stage. Correction with hypoglycemic agents in the 3rd group had a positive effect, but was not able to reduce the level of primary products of lipid peroxidation and restore antioxidant activity, so the use of additional agents became necessary. The use of aflibercept and a nitric oxide donor in the 4th group to correct the development of diabetic retinopathy significantly suppressed oxidative stress, the maximum of which occurred on the 180th day of the experiment, but did not reach control indicators. It was proven that the combined administration of bromfenac and aflibercept in the 5th group significantly reduced the number of primary LPO products, but not as significantly as in the 4th group. It was proved that the administration of aflibercept, L-carnitine and bromfenac to the animals of the 6th group reduced the content of diene conjugates already on the 30th and continued on the 60th and 180th days of the study, but it also did not reach the control indicators. The combination of metformin, aflibercept, L-arginine and citicoline in rats of the 7th group proved to be the most effective correction, as evidenced by the normalization of the level of the studied indicators on the 30th and 60th days of the experiment, and on the 180th, a decrease in the content of markers was detected oxidative stress to control indicators.
Dynamic of changes in the level of Interleukin 1B against the background of the development of experimental diabetic retinopathy and under the influence of various methods of correction
Authors: Sirman Y. , Savytskyi I. , Preys N. , Blavatska O. 
Published: September 2022

The aim of our research is investigating the changes in the level of interleukin 1β in experimental animals, which were simulated diabetic retinopathy and against the background of its correction. The obtained indicate that already on the 30th day of the development of experimental diabetic retinopathy, a significant increase in the pro-inflammatory cytokine level draws attention. Analyzing the data of group No. 3, it was established that the correction of the pathological condition with hypoglycemic agents has a positive effect but requires the involvement of additional means of correction in addition to the normalization of the level of glycemia. The results of the 4th experimental group indicate that the involvement of a nitric oxide donor and aflibercept in the correction of diabetic retinopathy has a positive effect on the reduction of the level of Interleukin 1β, but it does not reach an approximation to the norm. It is observed that the correction of the simulated pathological condition by reducing hyperglycemia, administration of aflibercept and bromfenac (group No. 5) gives positive results, but less pronounced than involvement in the complex correction of L-arginine solution in the study of nitric oxide synthases, in the analysis of the pro-inflammatory cytokine level the results are better. It is also worth noting that this method of correction is not characterized by longevity and on the 180th day there is already a pronounced decrease in its effectiveness. It was found that in rats in which diabetic retinopathy was modeled with subsequent correction of hyperglycemia, administration of aflibercept, L-carnitine and bromfenac (group No. 6), there is a pronounced effectiveness of the proposed method of correction in comparison with the previously considered methods.
As for the correction applied in the 7th group, in the first and second stages, the inflammation indicator is more elevated compared to the two previous groups (No. 5 and No. 6), but in the third stage, a marked decrease in the level of the marker was established, which indicates the effectiveness of the correction of the 7th group at more distant stages of the experiment.
The Sinatra Doctrine: bespoke approaches to academic research in surgery
Authors: Mellor K , Powell AGMT , Luton O , James OP , Egan RJ , Lewis WG 
Published: September 2022

Introduction Academic scholarly credentials are now obtained in two broad ways: out of programme research (OOPR) experience via bespoke funding (BF) or a higher degree (HD) funded by run-through fellowships (academic clinical fellow/lecturer in England or Wales Clinical Academic Track [WCAT]). The aim of this study was to determine the relative academic credentials achieved related to HD route, surgical specialty and topic. Methods Fifty-four postgraduate trainees engaged in HD study were followed and the primary outcome measures were HD completion, publication number and Hirsch index (HI). Results There were 38 BF trainees and 16 WCAT trainees. General surgical trainees were more likely to choose postgraduate research (n = 37, 68.5%) than other specialties (n = 17, 31.5%). Similarly, trainees in general surgery were more likely to choose the BF route than the WCAT pathway (94.6% vs 12.5%, (p < 0.001), with ethnic minorities also more likely to choose WCAT (56.3% vs 18.4%, p = 0.005). HD completion rates were 82.1% in the BF cohort and 88.9% in the WCAT group (p = 0.409). The median number of publications was 17 versus 18 respectively (p = 0.894) and the median HI was 7 in both groups (p = 0.690). OOPR experience during phase 1 of surgical training was associated with a higher HI (11 vs 0, p = 0.011). Degree type (MD vs PhD) was not associated with HD completion (80.0% vs 91.7%, p = 0.374), publication number (15 vs 19, p = 0.958) or HI (7 vs 5, p = 0.107). Conclusions HD routes appear equivalent although specialty related HI outcomes varied over fourfold. Trainees and training programme directors alike should be aware of these outcomes when planning OOPR training. Surgical training is multifaceted and should result in a competent clinician, able scholar, first class communicator, health advocate and well rounded professional. 1 Surgical research has served as the foundation for evidence-based clinical practice as long ago as Galen. 2 Nevertheless, in the UK and since the inception of Modernising Medical Careers, out of programme research (OOPR) experience has lost its allure. 3 , 4 Indeed, other distinguishing credentials are available, such as subspecialty clinical attachments, and qualifications in management, education and leadership.
Co-production of an information video on silver diammine fluoride – with children, for children
Authors: Timms Laura , Marshman Zoe , Deery Chris , Rodd Helen 
Published: September 2022

Introduction Silver diammine fluoride (SDF) is a relatively recent introduction to clinical practice in the UK as a caries management option for children’s primary teeth. However, implementation and uptake are likely to be improved by the availability of child focused information, which is key to ensuring that children understand information about their treatment choices. This study engaged children to co-produce an information video about SDF and explored other children’s opinions about the video. Methods A video was co-produced with children that explained caries management options with a focus on SDF. Children created the content and had substantial input into the editing process. Qualitative semi-structured interviews were carried out with 16 children, aged 4–8 years, based on a topic guide to explore their views on the video. Framework analysis was then performed. Results The video was viewed positively overall although participants did offer diverse views on some specific aspects. The data could be summarised through the following themes: video style and format, caries specific information and views on SDF. Conclusions Our study showed that children recognise the value and utility of an information video co-produced by their peers for provision of information about SDF as a caries management option.
Factors associated with the timely completion of doctoral research studies in clinical pharmacy: A mixed-methods study
Authors: Mosanya Adaobi U. , Ukoha-kalu Blessing O. , Isah Abdulmuminu , Umeh Ifeoma , Amorha Kosisochi C. , Ayogu Ebere E. , Ubaka Chukwuemeka 
Published: September 2022

Introduction
There is growing scientific evidence of mental and well-being issues that doctoral research students face as a result of not finishing their program on time. This study aims to explore the factors associated with the timely completion of doctoral research studies in the clinical pharmacy speciality.

Methods
This was a mixed-method study that combined surveys with in-depth interviews. Current doctoral research students and pharmacists who have recently completed their doctoral research program participated in the study. A validated questionnaire and an interview topic guide developed from the literature and pretesting were used to collect data. Data for this study were collected between February 2021 and September 2021. Quantitative data were analysed with the Statistical Package for Social Sciences (SPSS) V.25 while interview data were subjected to reflexive thematic analysis.

Results
47 students who are currently pursuing their doctoral research program in clinical pharmacy participated in the survey, while 8 pharmacists who had recently completed their doctoral research program in clinical pharmacy participated in the in-depth interviews. Five themes were identified: factors contributing to delay in the program, factors contributing to the timely completion of the program, ways to improve the program, advice to current students and advice to prospective students. Having more than one supervisor, supervisors’ commitment to the research work and support from the department were identified as facilitators of timely completion of doctoral research programs in clinical pharmacy.

Conclusion
Our study provides an understanding of the barriers and facilitators of timely completion of doctoral research programs in the clinical pharmacy specialist, and how these can be used to improve the postgraduate study programs in Nigeria.
Compliance to HIV testing and counseling guidelines at antenatal care clinics in the Kassena-Nankana districts of northern Ghana: A qualitative study
Authors: Choi Wooyon Annette , Sakeah Evelyn , Oduro Abraham Rexford , Aburiya John Bosco Achana , Aborigo Raymond Akawire 
Published: September 2022

Background
Utilization of antenatal care services in Ghana has substantially increased over the years, but the rates of mother-to-child transmission of HIV is still high. The high burden of HIV among pregnant women has serious implications for mother-to-child transmission. The main objective of this study was to assess the compliance of HIV testing and counseling provided at antenatal care clinics in two rural districts in northern Ghana by comparing reported practices to the national guidelines.

Methods
This study was a descriptive qualitative study conducted in the Kassena-Nankana Districts of northern Ghana. In-depth interviews were conducted with 10 midwives, 10 mothers, and 2 public health nurses who were recruited through purposive and snowball sampling. All interviews were audio recorded, transcribed into English, and imported into NVivo 12.0 software for open, axial, and selective coding.

Results
The findings indicate that not all pregnant women were informed prior to testing nor informed of their test results. Many mothers indicated that pre-test counseling is limited although the midwives claimed to provide it. Post-test counseling is primarily given to those who test positive, and several midwives agreed that there is no need to counsel HIV-negative women. Perceptions of the lack of confidentiality and privacy were pervasive among mothers despite the emphasis placed on its importance by the midwives. There were conflicting reports on whether HIV testing during antenatal care is voluntary or compulsory. The challenges with HIV testing and counseling that were mentioned by midwives include lack of adequate infrastructure, language barriers, and insufficient training.

Conclusions
HIV testing and counseling provided at antenatal care is not uniform across all health facilities and does not strictly adhere to national guidelines. Future interventions that focus on standardization, monitoring, privacy, and capacity building are likely to prove valuable in ensuring quality services are provided.
Effectiveness of shengxuexiaoban capsules combined with glucocorticoid therapy for immune thrombocytopenia: A meta-analysis
Authors: Ding Meng-Yuan , Li Bing , Yang Meng , Zhai Wen-Sheng , Song Chun-Dong , Zhang Jian , Zhang Qiu-Yue , Li Peng-Fei , Liu Li-Ya 
Published: September 2022

Objective
To evaluate the effectiveness of the Shengxuexiaoban Capsules combined with glucocorticoid therapy for immune thrombocytopenia (ITP).

Methods
We collected randomized controlled trials (RCTs) using shengxuexiaoban capsules in combination with glucocorticoid to treat ITP by searching major Chinese and English electronic databases. The outcome indicators were the effective rate, recurrence rate, the number of platelets in the blood, recovery time of platelets, and adverse reactions. We used STATA 16.0 and RevMan 5.3 for meta-analysis and GRADE pro. for evidence quality evaluation.

Results
A total of 27 RCTs were included in the meta-analysis, and the results showed a significant difference (all p<0.05) in the effective rate, recurrence rate, the number of platelets, and the recovery time of platelets (≥ 100×109) between ITP patients in the control group (who received glucocorticoid therapy alone) and test group (who received glucocorticoid therapy combined with the Shengxuexiaoban Capsules). And that Shengxuexiaoban capsules combined with glucocorticoid therapy were safe. The funnel plot and Egger’s test results indicated no obvious publication bias. The GRADE evidence rating showed an intermediate quality of evidence rating for recurrence rate and overall effectiveness.

Conclusion
Glucocorticoid therapy combined with the Shengxuexiaoban Capsules showed more effectiveness in the treatment of ITP. It can improve the effective rate, reduce the recurrence rate, increase the number of platelets and shorten the recovery time of platelets, and has a good safety profile.