bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does added plant residue interfere with the profiling of soil bacterial community by 16S rRNA gene sequencing and qPCR?
Authors: Yuan Chaolei , Sun Zhaoyang , Li Jing 
Published: September 2022

Abstract
Many studies evaluate the effect of plant residue on soil bacteria by comparing the microbial communities in soils with or without added plant residue after a period of incubation. This comparison can be made, however, only when the measured microbial properties are not significantly different between the amended soil and the control on day 0. To confirm this, we mixed plant residue into soil and immediately (within < 10 min; representing day 0) started DNA extraction, and then performed 16S rRNA gene sequencing and quantitative PCR (qPCR) to determine bacterial community composition and abundance. Unamended soil served as a control. Five commonly used DNA extraction kits, 16S rRNA gene primer pairs, and soils, and two types (mature rice straw and alfalfa shoots) and three addition rates (2%, 4%, and 6%; w/w) of plant residue, were tested. In all cases, we found no significant difference in bacterial community composition or abundance between the treatments with or without added plant residue. The results can help us better interpret differences in soil bacterial communities between the treatments with or without added plant residue in soil incubation experiments.
The predictive value of CD4, CD8, and C - reactive protein in the prognosis of schistosomal and non-schistosomal colorectal cancer
Authors: Cheng Meihong , Jing Hongyan , Bu Dacheng , Liu Jing , Lu Kui , Liu Jican , Xu Yanchao , Zhu Ting , Hou Yingyong , Yao Junxia , Zhai Qilin , Wang Weixia 
Published: September 2022

Abstract
Background: Although schistosomiasis has been basically eliminated, it has not been completely extinction in China and occasional outbreaks occur in Europe in recent years. The relationship between inflammation caused by Schistosoma japonicum and colorectal cancer is still obscure, and the inflammation based prognostic systems of schistosomal colorectal (SCRC) has rarely been reported.
Aim: To explore the different roles of tumor infiltrating lymphocytes (TILs) and C-reactive protein (CRP) in SCRC and in Non-schistosomal CRC (NSCRC), providing a possible predictive system to evaluate outcomes and to improve the risk stratification for CRC patients, especially for CRC patients with schistosomiasis.
Methods: 351 CRC tumours were evaluated for density of CD4+, CD8+ T cells and CRP in intratumoural and stromal compartments by immunohistochemical using tissue microarray.
Results: There were no association between TILs and CRP and schistosomiasis. Multivariate analysis identified stromal CD4 (sCD4) (p=0.038), intratumoural CD8 (iCD8) (p=0.003), schistosomiasis (p=0.045) as independent prognostic factors for overall survival (OS) in the whole cohort; and sCD4 (p=0.006) and iCD8 (p=0.020) were independent prognostic factors for OS in the NSCRC and SCRC set, respectively. Besides, we found that there were no differences of TILs and CRP, which were distributed in different areas of tumor tissue, between CRC patients with and without schistosomiasis.
Conclusion: The results remind us that different subtypes of TILs have distinguished biological behavior and function in the immune microenviroment of NSCRC and SCRC patients. Meanwhile, the findings require us to stratify patients with schistosomiasis and might facilitate patient counseling and management.
On an effective and efficient method for exploiting the wisdom of the inner crowd
Authors: Fujisaki Itsuki , Yang Kunhao , Ueda Kazuhiro 
Published: September 2022

Abstract
Researchers have shown that even an individual can produce the wisdom of the crowds, called ‘the wisdom of the inner crowd’. However, the previous methods leave room for improvements in terms of efficacy and convenience. This paper proposes a more efficient method with low cognitive cost, based on findings from cognitive and social psychology. The procedure is to ask participants to give two answers to the same question: first, their own estimate and, second, their estimate of public opinion. Experiments using this method showed that the averages of the two estimates were more accurate than the participants’ first estimates. That is, the wisdom of the inner crowd emerged. In addition, we found that the method could be superior to other methods in terms of efficacy and convenience. Moreover, we identified the conditions where our method worked better. We further clarify the limitations of using the wisdom of the inner crowd, including people’s tendency to fall into overconfidence. Overall, this paper proposes an effective and convenient method for harvesting the wisdom of the inner crowd.
Genome Editing Platforms in Rice (Oryza sativa L.): Basic methodology and troubleshooting
Authors: Kumari Diksha , Prasad Bishun , Dwivedi Padmanabh 
Published: September 2022

Abstract
Nowadays, genome editing approaches are one of the most frequently used for studying the function of a new gene(s) and for the development of elite mutant lines with desired traits. The technology has to boost up the craze among the researchers for editing the crop genome. However, information regarding the constructions of CRISPR/Cas9 gene cassette to develop edited rice plants is scattered. In the present study, we have shown a systematic stepwise protocol for designing gRNA, cloning of gRNA in CRISPR/Cas9 binary vector, Agrobacterium-mediated transformation, screening and confirmation of edited plants along with troubleshooting at each step to accelerate the application of the CRISPR/Cas9 system for rice improvement. The CHOPCHOP web tool was used for designing primers for gRNA. In this study, we are mentioning a specific trait for gene editing because we are giving overall easy and efficient protocols for generating edited plants for any trait. Plants with the presence of CaMV35S promoter, OsU3 promoter, PAT gene, and Cas9 gene were treated as gene-edited plants whereas the absence of the desired band in plants was treated as wild type. The performance of genome editing technology in the laboratory depends upon the systematic steps to finally find the desirable edited plant, and this simplified method of CRISPR/Cas9 mediated gene editing will accelerate functional genomics studies in rice.
Effects of handover education using the OPT model and SBAR protocol in nursing students: A quasi-experimental design
Authors: Seo Yon Hee , Jang Kyeongmin , Ahn Jung-Won 
Published: September 2022

Abstract
Background
Since most of nursing students lack clinical reasoning competency, for effective handover education, it is necessary to include a strategy to improve the clinical reasoning competency of nursing students in the practical training course of the nursing department.
Methods
This study is a quasi-experimental research design using a non-equivalent control group pretest-posttest design to verify the effectiveness of the practice education program using the OPT model and SBAR. The subjects of this study were 73 third-year students from two universities participating in internal medicine ward practice or clinical alternative practice.
Results
Homogeneity was ensured in all variables of the experimental group and the control group. The experimental group showed significantly better communication clarity (t=-12.262, p =<.001), communication confidence (t =-12.486, p=<.001), problem-solving processes (t =-13.100, p =<.001), and team efficacy (t = -6.197, p = <.001) compared to before the intervention. However, there was no significant difference between the pre- and post-intervention scores of the control group.
Conclusion
In a situation where clinical practice is difficult for nursing students, the handover education program using the OPT model and SBAR protocols can helps improve nursing students' communication clarity, communication confidence, problem- solving process, and team efficacy. Further studies are required to confirm these results.
A power-efficient integrated lithium niobate electro-optic comb generator
Authors: ZHANG Ke , Sun Wenzhao , Chen Yikun , Feng Hanke , Zhang Yiwen , Chen Zhaoxi , Wang Cheng 
Published: September 2022

Abstract
Integrated electro-optic (EO) frequency combs are essential components for future applications in optical communications, light detection and ranging, optical computation, sensing and spectroscopy. To date, broadband on-chip EO combs are typically generated in high-quality-factor micro-resonators, while the more straightforward and flexible non-resonant method, usually using single or cascaded EO phase modulators, often requires high driving power to realize a reasonably strong modulation index. Here, we show that the phase modulation efficiency of an integrated lithium niobate modulator could be dramatically enhanced by passing optical signals through the modulation electrodes for a total of 4 round trips, via multiple low-loss TE0/TE1 mode multiplexers and waveguide crossings, reducing electrical power consumption by more than one order of magnitude. Using devices fabricated from a wafer-scale stepper lithography process, we demonstrate a broadband optical frequency comb featuring 47 comb lines at a 25-GHz repetition rate, using a moderate RF driving power of 28 dBm (0.63 W). Leveraging the excellent tunability in repetition rate and operation wavelength, our power-efficient EO comb generator could serve as a compact low-cost solution for future high-speed data transmission, sensing and spectroscopy, as well as classical and quantum optical computation systems.
WITHDRAWN: A novel model of the deep neural network approach in coal mining surface pattern to assess land use classification using remote sensing image
Authors: Kumar Ajay , Das Bhumika 
Published: September 2022

Abstract
Computer vision usage in coal mining land use (LU) pattern classification is an exigent application for accurate accuracy. In the context of the satellite image pattern classification, the artificial intelligence (AI) for the development of a deep neural network (DNN), we have expressed an interest in land use (LU) for coal mining activities. Also, this image is addressed an area of interest as a spatial feature pattern that characterises coal mining regions. Moreover, the DNN classification algorithm's performance depends on the quality of the dataset. In the satellite image dataset, these practices of supervised-based learning are used for the accuracy assessment of mining activities area that is categorized into five classes coal area, built-up area, barren area, vegetation area, and water area respectively. The suit of mining activities area is selected from a case study of Talcher, Odisha, India. Further, we have found performances of training, testing, and validation like 88%, 69.7%, and 73.6%, respectively. Also, the overall accuracy is 79.4%. Therefore, the potential of DNNs learning is introduced for LU classification over mining activities area.
A social media fix to the Wallacean shortfall
Authors: Chowdhury Shawan , Aich Upama , Rokonuzzaman Md. , Alam Shofiul , Das Priyanka , Siddika Asma , Ahmed Sultan , Labi Mahzabin , Marco Moreno Di , Fuller Richard , Callaghan Corey 
Published: September 2022

Abstract
Citizen science programmes are becoming increasingly sophisticated and popular among those with an interest in natural history, but remain the domain of a relatively small portion of the public and heavily biased toward certain areas. Although systematic recording of biodiversity data has been practiced for centuries in the Global North, many tropical nations are still vastly under-surveyed. Yet with the explosive popularity of social media, and the near-ubiquitous availability of smartphone cameras, millions of people are posting photographs on social media daily. Here, we illustrate the potential of harvesting these data to enhance our biodiversity understanding using Bangladesh, a megadiverse South Asian nation, as a case study. We compared biodiversity records extracted from Facebook with those from the Global Biodiversity Information Facility (GBIF) collating geospatial records for 1,013 unique species, including 970 species from Facebook (representing 25% of observation records) and 712 species in GBIF (representing 75% of observation records). While a vast proportion of the combined spatial data were biased towards major cities, Facebook records were more evenly spatially distributed compared to those from GBIF. About 86% of the available distribution records on threatened species were from Facebook, whereas GBIF records were almost entirely of Least Concern species. Our results reveal that social media archives can contain biodiversity data that far eclipses that available from formal databases in terms of volume, and also complements formal data both taxonomically and spatially. A key research priority now is the development of mechanisms for extracting and interpreting social media biodiversity data.
Antiseizure medication use after meningioma resection: A single institution experience
Authors: Ellis Erin , Drumm Michael R. , Rai Samhitha , Huang Jonathan , Tate Matthew C. , Magill Stephen T. , Templer Jessica W. 
Published: September 2022

Abstract
Objective: The objective of this study was to investigate the antiseizure medication (ASM) practice behavior at a single institution with regards to type, dosage, and frequency in patients who present with seizures prior to meningioma resection and to review post-operative ASM management.
Methods: A retrospective study of 112 consecutive patients with meningiomas who underwent resection at a single institution between October 2016 and January 2020. Data was collected through detailed chart review, and included demographics, pre-and post-operative seizure characteristics, and type, dose, frequency, and changes to ASM regimens.
Results:Thirty-five patients (31%) had a preoperative seizure, and 43 (38%) of patients were prescribed a preoperative ASM. At discharge, 96 (86%) of patients were prescribed an ASM, which was most often 1000 mg daily total of levetiracetam (64%, 61/96), and less often higher doses of levetiracetam or other ASMs. By the six-month postoperative visit, 55 (49%) of patients were taking at least one ASM, and this number of patients further decreased to 45 (40%) and 36 (32%) by one-year follow-up and last-known follow-up, respectively. Median time to last follow-up was 27.3 months (range = 5.4 – 57.4 months), and by last follow-up, 24 (21%) of patients had experienced a post-operative seizure, and 36 (32%) of patients were never able to discontinue their ASMs.
Conclusions: Most patients who underwent surgical resection of their meningioma were eventually able to completely discontinue their postoperative ASM regimen. However, a large minority of patients continued to experience postoperative seizures throughout their treatment course and required long-term management.
Extent of ICT Integration in Science Based on SAMR Model
Authors: Flores Den Karl , Adlaon Mauricio 
Published: September 2022

Abstract
This study presents the level of ICT integration of science teachers based on the SAMR model of Ruben R. Puentedura. The said model was put into the context of science education during the first semester of online distance learning school year 2021–2022. This study involves the participation of science teachers and students. The components of the SAMR model are used as an indicator of this study. There were 54 science teachers and 591 students who participated in this study; each respondent was given a researcher-made questionnaire which is validated by the experts and tested for internal consistency. The data gathered were analyzed using the following descriptive statistical tools: (a)Frequency Count and Percentage Distribution, (b) Mean and Standard Deviation, and (c) Analysis of Variance (ANOVA). Based on the results Science teachers’ ICT integration is highly evident in the SAMR model’s Augmentation level. This is confirmed by the students. In the aforementioned model, augmentation is the second of four rungs. Science teachers utilized digital technology with improved functional change instead of traditional tools. It is in the enhancement of the Information and Communication Technology level but still needs to be upscaled to reach the transformational level where the use of ICT technology improves students' learning. The age and years of teaching experience of Science teachers have an impact on their ICT integration. Students’ perceptions of ICT integration in Science classes, on the other hand, are influenced by their curriculum, grade level, and school.
A framework to estimate continuous streamflow at the ungauged site with limited catchment’s hydrological data: Case study Isahaya Catchment
Authors: Angalika Misigo W. S. , Suzuki Seiji , Tanaka Wataru , Vu Huynh V. , Itayama Tomoaki 
Published: September 2022

Abstract
This paper presents a framework for mapping flow information from the gauged to the ungauged river basins. The calibration and validation of a hydrologic model were conducted to establish basic watershed characteristics. The new framework was then applied to account for the two watersheds' proportionality in their similarity, such as the influence of land use on transplanting flow signatures. Three land-use scenarios-discharges at the ungauged and gauged sites formed the basis of an equation mapping the gauged discharge signal to the ungauged site. In comparison with intermittent observed data, the framework prediction attained a precision of 0.85≥NSE≤0.95, 0.80≥R2≤0.94, 0.56≥bR2≤0.89. Despite considerable differences in the watershed area, slope, soils, and land cover, the framework satisfactorily depicted the variation in flow pulses of each river. In the absence of established hydrological information, this provides an alternative flow estimation at ungauged sites, reducing uncertainties in the regionalization of model parameters.
Exchange, catalysis and amplification of encapsulated RNA driven by periodic temperature changes
Authors: Salibi Elia , Peter Benedikt , Schwille Petra , Mutschler Hannes 
Published: September 2022

Abstract
Growth and division of biological cells is based on the complex orchestration of spatiotemporally controlled reactions driven by highly evolved proteins. In contrast, it remains unknown how their primordial predecessors could achieve a stable inheritance of cytosolic components before the advent of translation. An attractive scenario assumes that periodic changes of environmental conditions acted as pacemakers for the proliferation of early protocells. Using catalytic RNA (ribozymes) as models for primitive biocatalytic molecules, we demonstrate that the repeated freezing and thawing of aqueous solutions enables the assembly of active ribozymes from inactive precursors encapsulated in separate lipid vesicle populations. Furthermore, we show that encapsulated ligase ribozymes can overcome freezing-induced content loss and successive dilution by freeze-thaw driven propagation in feedstock vesicles. Thus, cyclic freezing and melting of aqueous solvents – a plausible physicochemical driver likely present on early Earth – provides a simple scenario that uncouples compartment growth and division from nucleic acid self-replication, while maintaining the propagation of these replicators inside new vesicle populations.
Effects of lesion size on the 30-month clinical performance of restorations of non-carious cervical lesions
Authors: correia ayla macyelle de oliveira , jurema ana luiza barbosa , bresciani eduardo , caneppele taciana marco ferraz 
Published: September 2022

Abstract
Objective: The aim of this double-blind, randomized controlled trial was to evaluate the influence of the occlusogingival distance (OGD) in noncarious cervical lesions (NCCLs) on the clinical performance of a regular bulk-fill resin composite and a regular nanofilled resin composite.
Materials and Methods: 140 NCCLs in 77 participants were randomly divided into four groups (n=35), according to OGD (1.5 mm±10% or 3 mm±10%) and resin composites (Filtek Bulk Fill Posterior [B] or Filtek Z350 XT [C]), namely: 1.5 mm-B, 1.5 mm-C, 3 mm-B, and 3 mm-C. The restorations were bonded using a two-step self-etch adhesive (Clearfil SE Bond), applied following the manufacturer’s instructions. Restorations were polished 1 week after placement. Two experienced and calibrated examiners evaluated the restorations using modified US Public Health Service criteria at baseline (7 days) and after 6, 12, 18, 24 and 30 months. Statistical analyses were carried out using Kruskal-Wallis, Friedman's repeated measures analysis of variance and the Wilcoxon Signed rank test (α = 0.05).
Results: After 30 months, the recall rate was 94.2%. Eight restorations were lost (3 for 1.5 mm-C, 2 for 1.5 mm-B, 1 for 3 mm-C and 2 for 3 mm-B). All groups resulted in a significantly worse marginal discoloration and surface texture at 30 months in comparison with the baseline (one week).
Conclusion: The restorations performed with both resin composites produced clinically acceptable restorations. The OGD of NCCLs of did not influence the clinical performance of restorations.
Clinical significance: The regular bulk-fill material showed excellent clinical behavior in NCCLs of different sizes, similar to the regular nanofilled resin composite.
Immunological characteristics of CD103+CD8+T cells in the liver of C57BL/6 mouse infected with plasmodium NSM
Authors: Shi Feihu , Chen Dianhui , Mo Feng , Li Jiajie , Fang Chao , Wei Haixia , Xing Junmin , Liu Lin , Gong Yumei , Tan Zhengrong , Pan Xingfei , Zhao Shan , Huang Jun 
Published: September 2022

Abstract
Background: CD103 is an important marker of tissue-resident memory T cells (TRM) which play important roles in fighting against infection. However, the immunological characteristics of CD103+ T cells are not thoroughly elucidated in the liver of mouse infected with Plasmodium.
Methods: 6-8 weeks old C57BL/6 mice were infected with Plasmodium yoelii nigeriensis NSM. Mice were anesthetized and sacrificed at 12 to 16 days after infection, and the livers were picked out. Sections of the livers were stained, and serum AST and ALT levels were measured. Moreover, lymphocytes in the liver were isolated, and the expression of CD103 was determined by using qPCR. The percentage of CD103 on different immune cell populations was dynamically observed by using flow cytometry (FCM). In addition, the phenotype and cytokine production characteristics of CD103+CD8+ T cell were analyzed by using flow cytometry, respectively.
Results: Erythrocyte stage plasmodium infection could result in severe hepatic damage, a widespread inflammatory response and the decrease of CD103 expression on hepatic immune cells. CD103 expression was increased on CD8+ T and γδ T cells. The decrease of CD103 expression was significantly on CD8+ T cells. Compared to that of CD103- CD8+ T cells, CD103+ CD8+ T cells from the infected mice expressed lower level of CD69, higher level of CD62L, and secreted more IL-4, IL-10, IL-17, and secreted less IFN-γ.
Conclusion:CD103+CD8+ T cells might mediate the hepatic immune response by secreting IL-4, IL-10, and IL-17 except IFN-γ in the mice infected with the erythrocytic phase plasmodium, which could be involved in the pathogenesis of severe liver damage resulted from the erythrocytic phase plasmodium yoelii nigeriensis NSM infection.