bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tribology of the sock‐skin Interface – the influence of different fabric parameters on sock friction
Authors: DeBois Ian J. , Agarwal Esha , Kapoor Ashish , Mathur Kavita 
Published: August 2022

AbstractBackgroundThe purpose of this parametric design of experiments was to identify and summarize how the influence of knit structure (single jersey vs. terry), fiber composition (polyester vs. cotton), fiber linear density (30/1 Ne vs. 18/1 Ne & 1/150/34 vs. 2/150/34), and yarn type (filament vs. spun) affected the frictional profile across the sock‐skin interface.MethodsFriction testing trials were completed against both a polypropylene probe and a synthetic skin material (Lorica soft®) to determine if there was a difference in friction based on interface interaction. Friction testing was completed by sliding a probe across the inside bottom surface of the sock (the part that is usually in‐contact with the bottom of the foot) while instantaneously measuring the frictional force every tenth of a second.ResultsFor both trials (plastic probe and synthetic skin), in the dry condition, knit structure was found to be the most prominent fabric parameter affecting the frictional force experienced at the sock‐skin interface. It was also determined that fiber linear density, and yarn type are tertiary factors affecting the frictional force measured at the sock‐skin interface. Finally, in the dry state, it was determined that fiber composition had seemingly no effect on the frictional force experienced at the sock‐skin interface.ConclusionThis parametric design of experiments has further enhanced the understanding of the tribology at the sock‐skin interface. Through strategic design, four different textile parameters have been investigated, measured, and justified as to how each influence the friction measured between the two interfaces. This knowledge can be used to develop socks that mitigate the risk of friction blisters formation.
Subphenotypes in acute kidney injury: a narrative review
Authors: Vaara Suvi T. , Bhatraju Pavan K. , Stanski Natalja L. , McMahon Blaithin A. , Liu Kathleen , Joannidis Michael , Bagshaw Sean M. 
Published: August 2022

AbstractAcute kidney injury (AKI) is a frequently encountered syndrome especially among the critically ill. Current diagnosis of AKI is based on acute deterioration of kidney function, indicated by an increase in creatinine and/or reduced urine output. However, this syndromic definition encompasses a wide variety of distinct clinical features, varying pathophysiology, etiology and risk factors, and finally very different short- and long-term outcomes. Lumping all AKI together may conceal unique pathophysiologic processes specific to certain AKI populations, and discovering these AKI subphenotypes might help to develop targeted therapies tackling unique pathophysiological processes. In this review, we discuss the concept of AKI subphenotypes, current knowledge regarding both clinical and biomarker-driven subphenotypes, interplay with AKI subphenotypes and other ICU syndromes, and potential future and clinical implications.
EvaLuation of early CRRT and beta-blocker InTervention in patients with ECMO (ELITE) trial: study protocol for a 2 × 2 partial factorial randomized controlled trial
Authors: Wang Xiaofang , Wang Hong , Du Xin , Wang Zhiyan , Li Chenglong , Anderson Craig S. , Zhang Jinying , Hou Xiaotong , Dong Jianzeng 
Published: August 2022

Abstract
Background
In critically ill patients requiring extracorporeal membrane oxygenation (ECMO) therapy, early initiation of continuous renal replacement therapy (CRRT) and beta-blockade of catecholamine-induced inotropic effects may improve outcomes.

Methods
A 2 × 2 partial factorial randomized controlled trial in eligible ECMO patients without a clear indication or contraindication to either intervention is centrally randomly assigned to (A) early or conventional-indicated CRRT and/or (B) beta-blocker or usual care. The primary outcome is all-cause mortality at 30 days for both arms. A total of 496 participants provides 80% power to determine a 20% risk reduction in mortality at 30 days with 5% type I error.

Discussion
This trial will help define the role of early CRRT and beta-blockade in ECMO patients. There have been 89 patients enrolled at 10 hospitals in study A and is ongoing. However, study B was stopped in August 2019 in the absence of any patients being enrolled.

Trial registration
ClinicalTrials.govNCT03549923. Registered on 8 June 2018. World Health Organization International Clinical Trials Registry Platform (WHO ICTEP) network. The Ethics Committee of Beijing Anzhen Hospital Approval ID is 2018013.
Application of green first derivative synchronous spectrofluorometric method for quantitative analysis of fexofenadine hydrochloride and pseudoephedrine hydrochloride in pharmaceutical preparation and spiked human plasma
Authors: Ramzy Sherif , Abdelazim Ahmed H. , Hasan Mohamed A. 
Published: August 2022

AbstractFexofenadine hydrochloride and pseudoephedrine hydrochloride are prescribed in a combined dosage form for the treatment of allergic rhinitis. In the present work, a sensitive synchronous fluorescence spectroscopic method was applied in conjunction with first derivative for quantitative estimation of fexofenadine hydrochloride and pseudoephedrine hydrochloride in pure form, pharmaceutical tablets and spiked human plasma. Fexofenadine hydrochloride showed its conventional emission spectrum at 294 nm when excited at 267 nm. On the other hand, pseudoephedrine hydrochloride showed its conventional emission spectra at 286 nm when excited at 261 nm. The fluorescence intensities were greatly enhanced by the use of sodium dodecyl sulphate as a micellar surfactant. Application of the synchronous mode to measure the fluorescence spectra of the above drugs provided sharp narrowing bands, but the overlap was not completely resolved. Derivatization of the synchronous spectra to the first order completely resolved the overlap of the fluorescence spectra and allowed simultaneous quantitative determination of the drugs under study. Fexofenadine hydrochloride and pseudoephedrine hydrochloride could be determined from their first-order synchronous spectra at 286 and 294 nm, respectively, without interfering with each other. The method showed linearity with an excellent correlation coefficient in the concentration range of 100–1500 ng/mL for Fexofenadine hydrochloride and 50–1000 ng/mL for pseudoephedrine hydrochloride. The method was successfully applied for the simultaneous determination of the studied drugs in pharmaceutical formulation, with mean percent recoveries for Fexofenadine hydrochloride and pseudoephedrine hydrochloride of 99.49 ± 0.931 and 98.67 ± 0.634, respectively, and in spiked human plasma, with mean percent recoveries for Fexofenadine hydrochloride and pseudoephedrine hydrochloride of 95.21 ± 1.938 and 94.89 ± 1.763, respectively. Furthermore, the greenness of the described method was assessed using four different tools namely, the national environmental method index, the analytical eco-scale, the green analytical procedure index and the AGREE evaluation method. The proposed method seemed to be superior to the reported HPLC method with respect to the metrics of the greenness characters.
PDS5A and PDS5B differentially affect gene expression without altering cohesin localization across the genome
Authors: Arruda Nicole L. , Bryan Audra F. , Dowen Jill M. 
Published: August 2022

Abstract
Background
Cohesin is an important structural regulator of the genome, regulating both three-dimensional genome organization and gene expression. The core cohesin trimer interacts with various HEAT repeat accessory subunits, yielding cohesin complexes of distinct compositions and potentially distinct functions. The roles of the two mutually exclusive HEAT repeat subunits PDS5A and PDS5B are not well understood.

Results
Here, we determine that PDS5A and PDS5B have highly similar localization patterns across the mouse embryonic stem cell (mESC) genome and they show a strong overlap with other cohesin HEAT repeat accessory subunits, STAG1 and STAG2. Using CRISPR/Cas9 genome editing to generate individual stable knockout lines for PDS5A and PDS5B, we find that loss of one PDS5 subunit does not alter the distribution of the other PDS5 subunit, nor the core cohesin complex. Both PDS5A and PDS5B are required for proper gene expression, yet they display only partially overlapping effects on gene targets. Remarkably, gene expression following dual depletion of the PDS5 HEAT repeat proteins does not completely overlap the gene expression changes caused by dual depletion of the STAG HEAT repeat proteins, despite the overlapping genomic distribution of all four proteins. Furthermore, dual loss of PDS5A and PDS5B decreases cohesin association with NIPBL and WAPL, reduces SMC3 acetylation, and does not alter overall levels of cohesin on the genome.

Conclusions
This work reveals the importance of PDS5A and PDS5B for proper cohesin function. Loss of either subunit has little effect on cohesin localization across the genome yet PDS5A and PDS5B are differentially required for gene expression.
Multiple horizontal transfers of a Helitron transposon associated with a parasitoid wasp
Authors: Heringer Pedro , Kuhn Gustavo C. S. 
Published: August 2022

AbstractIn a previous study we described a Helitron transposon that apparently became one of the segments in the symbiotic Cotesia vestalis bracovirus (CvBV) from the parasitoid wasp C. vestalis. We presented evidence that this Helitron, named Hel_c35, invaded the C. vestalis genome through a horizontal transfer (HT) event from a dipteran and was later transferred horizontally from C. vestalis to a lepidopteran species. Based on the phylogeny of Hel_c35, we suggested that both HTs occurred in East Asia. We have also anticipated that, as more sequenced genomes from new species become available, more HTs involving Hel_c35 would be detected. Although the inclusion of Hel_c35 as a CvBV segment turned out to be a methodological artifact, the fact that Hel_c35 copies are present in the genomes of C. vestalis and other arthropods still remains. Here, we investigated the evolution of Hel_c35 in arthropods using an updated data set to reassess our previous findings. Most species (95%) included in the present work had their genomes sequenced after our initial study was published, thus representing new descriptions of taxa harboring Hel_c35. Our results expand considerably the number of putative HTs involving Hel_c35, with up to dozens of previously undescribed events, and suggest that the most recent HTs associated with C. vestalis took place in Europe. Considering the phylogenetic distribution of Hel_c35, and the evidence that its DNA sequences are present in the calyx fluid of C. vestalis and tissues from its parasitized host, we argue that many HT events were favored by the behavior of this wasp.
Diabetic microenvironment preconditioning of adipose tissue-derived mesenchymal stem cells enhances their anti-diabetic, anti-long-term complications, and anti-inflammatory effects in type 2 diabetic rats
Authors: Su Wanlu , Yu Songyan , Yin Yaqi , Li Bing , Xue Jing , Wang Jie , Gu Yulin , Zhang Haixia , Lyu Zhaohui , Mu Yiming , Cheng Yu 
Published: August 2022

Abstract
Background
Mesenchymal stem cells (MSCs) exert anti-diabetic effects and improve long-term complications via secretory effects that regulate macrophage polarisation and attenuate inflammation. Enhancing the efficacy of MSCs needs to be explored further. The in vitro culture microenvironment influences the secretory profile of MSCs. Therefore, we hypothesised that a diabetic microenvironment would promote the secretion of cytokines responsible for macrophage polarisation, further attenuating systemic inflammation and enhancing the effects of MSCs on type 2 diabetes (T2D) and long-term diabetic complications.

Methods
Preconditioned adipose-derived mesenchymal stem cells (pre-ADSCs) were obtained after co-cultivating ADSCs in a diabetic metabolic environment (including high sugar, advanced glycation end-product, and lipopolysaccharides). The regulatory effects of pre-ADSCs on macrophages were observed in vitro. A T2D rat model was induced with a high-fat diet for 32 weeks combined with an intraperitoneal injection of streptozotocin. Sprague–Dawley (SD) rats were divided into four groups: normal group, diabetes without treatment group (PBS), ADSC treatment group, and pre-ADSC treatment group. ADSCs and pre-ADSCs were intravenously administered weekly to SD rats for 6 months, and then glucose homeostasis and long-term diabetic complications were evaluated in each group.

Results
The secretion of cytokines related to M2 macrophage polarisation (IL-6, MCP-1, etc.) was increased in the pre-ADSC group in the in vitro model. Pre-ADSC treatment significantly maintained blood glucose homeostasis, reduced insulin resistance, promoted islet regeneration, and ameliorated the complications related to diabetes in rats (chronic kidney disease, non-alcoholic steatohepatitis, lung fibrosis, and cataract) compared to the ADSC group (P < 0.05). Additionally, the number of anti-inflammatory M2 macrophage phenotypes was enhanced in tissues following pre-ADSC injections. Moreover, the expression of pro-inflammatory genes (iNOS, TNF-α, IL-1β) was reduced whereas that of anti-inflammatory genes (Arg1, CD206, and Il-10) was increased after cultivation with pre-ADSCs.

Conclusion
Diabetic microenvironment-preconditioned ADSCs effectively strengthen the capacity against inflammation and modulate the progress of long-term T2D complications.
Aucubin promotes bone-fracture healing via the dual effects of anti-oxidative damage and enhancing osteoblastogenesis of hBM-MSCs
Authors: Wang Kanbin , Zhou Chengwei , Li Lijun , Dai Chengxin , Wang Zhongxiang , Zhang Weijun , Xu Jianxiang , Zhu Yueliang , Pan Zhijun 
Published: August 2022

Abstract
Background
Aucubin (AU), an iridoid glucoside isolated from many traditional herbal medicines, has anti-osteoporosis and anti-apoptosis bioactivities. However, the effect of AU on the treatment of bone-fracture remains unknown. In the present study, the aims were to investigate the roles and mechanisms of AU not only on osteoblastogenesis of human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) and anti-oxidative stress injury in vitro, but also on bone-fracture regeneration by a rat tibial fracture model in vivo.

Methods
CCK-8 assay was used to assess the effect of AU on the viability and proliferation of hBM-MSCs. The expression of specific genes and proteins on osteogenesis, apoptosis and signaling pathways was measured by qRT-PCR, western blotting and immunofluorescence analysis. ALP staining and quantitative analysis were performed to evaluate ALP activity. ARS and quantitative analysis were performed to evaluate calcium deposition. DCFH-DA staining was used to assess the level of reactive oxygen species (ROS). A rat tibial fracture model was established to validate the therapeutic effect of AU in vivo. Micro-CT with quantitative analysis and histological evaluation were used to assess the therapeutic effect of AU locally injection at the fracture site.

Results
Our results revealed that AU did not affect the viability and proliferation of hBM-MSCs. Compared with control group, western blotting, PCR, ALP activity and calcium deposition proved that AU-treated groups promoted osteogenesis of hBM-MSCs. The ratio of phospho-Smad1/5/9 to total Smad also significantly increased after treatment of AU. AU-induced expression of BMP2 signaling target genes BMP2 and p-Smad1/5/9 as well as of osteogenic markers COL1A1 and RUNX2 was downregulated after treating with noggin and LDN193189. Furthermore, AU promoted the translocation of Nrf2 from cytoplasm to nucleus and the expression level of HO1 and NQO1 after oxidative damage. In a rat tibial fracture model, local injection of AU promoted bone regeneration.

Conclusions
Our study demonstrates the dual effects of AU in not only promoting bone-fracture healing by regulating osteogenesis of hBM-MSCs partly via canonical BMP2/Smads signaling pathway but also suppressing oxidative stress damage partly via Nrf2/HO1 signaling pathway.
Probabilistic volcanic hazard assessment at an active but under-monitored volcano: Ceboruco, Mexico
Authors: Constantinescu Robert , González-Zuccolotto Karime , Ferrés Dolors , Sieron Katrin , Siebe Claus , Connor Charles , Capra Lucia , Tonini Roberto 
Published: August 2022

AbstractA probabilistic volcanic hazard assessment (PVHA) for Ceboruco volcano (Mexico) is reported using PyBetVH, an e-tool based on the Bayesian Event Tree (BET) methodology. Like many volcanoes, Ceboruco is under-monitored. Despite several eruptions in the late Holocene and efforts by several university and government groups to create and sustain a monitoring network, this active volcano is monitored intermittently rather than continuously by dedicated groups. With no consistent monitoring data available, we look at the geology and the eruptive history to inform prior models used in the PVHA. We estimate the probability of a magmatic eruption within the next time window (1 year) of ~ 0.002. We show how the BET creates higher probabilities in the absence of monitoring data, which if available would better inform the prior distribution. That is, there is a cost in terms of higher probabilities and higher uncertainties for having not yet developed a sustained volcano monitoring network. Next, three scenarios are developed for magmatic eruptions:i)small magnitude (effusive/explosive), ii)medium magnitude (Vulcanian/sub-Plinian) andiii)large magnitude (Plinian). These scenarios are inferred from the Holocene history of the volcano, with their related hazardous phenomena: ballistics, tephra fallout, pyroclastic density currents, lahars and lava flows. We present absolute probability maps (unconditional in terms of eruption size and vent location) for a magmatic eruption at Ceboruco volcano. With PyBetVH we estimate and visualize the uncertainties associated with each probability map. Our intent is that probability maps and uncertainties will be useful to local authorities who need to understand the hazard when considering the development of long-term urban and land-use planning and short-term crisis management strategies, and to the scientific community in their efforts to sustain monitoring of this active volcano.
A network business availability modeling method based on Markov chain
Authors: Lu Ying , Chen Yusi , Liu Jin , Fu Yanfang , Guo Qi , Fan Nierui 
Published: August 2022

AbstractMilitary tactical networks (MTNs) have grown in size and complexity as the volume of battlefield communications business has increased dramatically. There is a critical need for a quantitative evaluation method to assess the performance of MTNs. Based on the transmission characters of battlefield communication business and the availability of communication equipment, this paper proposed a Markov chain-based network steady-state availability model. Using this model and the analysis of the CSMA/CA transmission protocol as preconditions, a delay-based quantitative evaluation model of network business availability is proposed based on statistical principles. The relationship between network business availability and crucial metrics such as node size and node failure/repair ratio is investigated in simulation experiments to ensure the validity and correctness of the proposed model. The work done in this paper contributes to effectively evaluating the overall availability of MTNs in a complex environment.
Association between treatment setting and outcomes among oregon medicaid patients with opioid use disorder: a retrospective cohort study
Authors: Hartung Daniel M. , Markwardt Sheila , Johnston Kirbee , Geddes Jonah , Baker Robin , Leichtling Gillian , Hildebran Christi , Chan Brian , Cook Ryan R. , McCarty Dennis , Ghitza Udi , Korthuis P. Todd 
Published: August 2022

Abstract
Background
Residential treatment is a common approach for treating opioid use disorder (OUD), however, few studies have directly compared it to outpatient treatment. The objective of this study was to compare OUD outcomes among individuals receiving residential and outpatient treatment.

Methods
A retrospective cohort study used linked data from a state Medicaid program, vital statistics, and the Substance Abuse and Mental Health Services Administration (SAMHSA) Treatment Episodes Dataset (TEDS) to compare OUD-related health outcomes among individuals treated in a residential or outpatient setting between 2014 and 2017. Multivariable Cox proportional hazards and logistic regression models examined the association between treatment setting and outcomes (i.e., opioid overdose, non-overdose opioid-related and all-cause emergency department (ED) visits, hospital admissions, and treatment retention) controlling for patient characteristics, co-morbidities, and use of medications for opioid use disorders (MOUD). Interaction models evaluated how MOUD use modified associations between treatment setting and outcomes.

Results
Of 3293 individuals treated for OUD, 957 (29%) received treatment in a residential facility. MOUD use was higher among those treated as an outpatient (43%) compared to residential (19%). The risk of opioid overdose (aHR 1.39; 95% CI 0.73–2.64) or an opioid-related emergency department encounter or admission (aHR 1.02; 95% CI 0.80–1.29) did not differ between treatment settings. Independent of setting, MOUD use was associated with a significant reduction in overdose risk (aHR 0.45; 95% CI 0.23–0.89). Residential care was associated with greater odds of retention at 6-months (aOR 1.71; 95% CI 1.32–2.21) but not 1-year. Residential treatment was only associated with improved retention for individuals not receiving MOUD (6-month aOR 2.05; 95% CI 1.56–2.71) with no benefit observed in those who received MOUD (aOR 0.75; 95% CI 0.46–1.29; interaction p = 0.001).

Conclusions
Relative to outpatient treatment, residential treatment was not associated with reductions in opioid overdose or opioid-related ED encounters/hospitalizations. Regardless of setting, MOUD use was associated with a significant reduction in opioid overdose risk.