bluuebunny/crossref_metadata_2025_split huggingface dataset:
Donation after circulatory death transplant outcomes using livers recovered by local surgeons
Authors: Jadlowiec Caroline C. , Macdonough Elizabeth , Pont Kylie , Valenti Kristi , Lizaola‐Mayo Blanca , Brooks Abigail , Das Devika , Heilman Raymond , Mathur Amit K. , Hewitt Winston , Moss Adyr , Aqel Bashar , Reddy Kunam S. 
Published: March 2022

Abstract
Donation after circulatory death (DCD) liver transplantation (LT) outcomes have been attributed to multiple variables, including procurement surgeon recovery techniques. Outcomes of 196 DCD LTs at Mayo Clinic Arizona were analyzed based on graft recovery by a surgeon from our center (transplant procurement team [TPT]) versus a local procurement surgeon (non‐TPT [NTPT]). A standard recovery technique was used for all TPT livers. The recovery technique used by the NTPT was left to the discretion of that surgeon. A total of 129 (65.8%) grafts were recovered by our TPT, 67 (34.2%) by the NTPT. Recipient age (p = 0.43), Model for End‐Stage Liver Disease score (median 17 vs. 18; p = 0.22), and donor warm ischemia time (median 21.0 vs. 21.5; p = 0.86) were similar between the TPT and NTPT groups. NTPT livers had longer cold ischemia times (6.5 vs. 5.0 median hours; p < 0.001). Early allograft dysfunction (80.6% vs. 76.1%; p = 0.42) and primary nonfunction (0.8% vs. 0.0%; p = 0.47) were similar. Ischemic cholangiopathy (IC) treated with endoscopy occurred in 18.6% and 11.9% of TPT and NTPT grafts (p = 0.23). At last follow‐up, approximately half of those requiring endoscopy were undergoing a stent‐free trial (58.3% TPT; 50.0% NTPT; p = 0.68). IC requiring re‐LT in the first year occurred in 0.8% (n = 1) of TPT and 3.0% (n = 2) of NTPT grafts (p = 0.23). There were no differences in patient (hazard ratio [HR], 1.95; 95% confidence interval [CI], 0.76–5.03; p = 0.23) or graft (HR, 1.99; 95% CI, 0.98–4.09; p = 0.10) survival rates. Graft survival at 1 year was 91.5% for TPT grafts and 95.5% for NTPT grafts. Excellent outcomes can be achieved using NTPT for the recovery of DCD livers. There may be an opportunity to expand the use of DCD livers in the United States by increasing the use of NTPT.
Clinical observation and genetic analysis of a SYNS1 family caused by novel NOG gene mutation
Authors: Zhang Zhao , Lu Yu , Cao Jing‐Yuan , Wang Li , Li Lin‐Ke , Wang Chao , Ye Xuan , Ji Yi‐Ming , Tu Lin‐Yi , Sun Yi 
Published: March 2022

AbstractObjectiveAnalyze the clinical and genetic characteristics of a rare Chinese family with Multiple synostoses syndrome and identify the causative variant with the high‐throughput sequencing approach.MethodsThe medical history investigation, physical examination, imaging examination, and audiological examination of the family members were performed. DNA samples were extracted from the family members. The candidate variant was identified by performing whole‐exome sequencing of the proband, then verified by Sanger sequencing in the family.ResultsThe family named HBSY‐018 from Hubei province had 18 subjects in three generations, and six subjects were diagnosed with conductive or mixed hearing loss. Meanwhile, characteristic features including short philtrum, hemicylindrical nose, and hypoplastic alae nasi were noticed among those patients. Symptoms of proximal interdigital joint adhesion and inflexibility were found. The family was diagnosed as Multiple synostoses syndrome type 1 (SYNS1).The inheritance pattern of this family was autosomal dominant. A novel mutation in the NOG gene c.533G>A was identified by performing whole‐exome sequencing of the proband. The substitution of cysteine encoding 178th position with tyrosine (p.Cys178Tyr) was caused by this mutation, which was conserved across species. Co‐segregation of disease phenotypes was demonstrated by the family verification.ConclusionThe family diagnosed as SYNS1 was caused by the novel mutation (c.533G>A) of NOG. The combination of clinical diagnosis and molecular diagnosis had improved the understanding of this rare disease and provided a scientific basis for genetic counseling in the family.
Delivery of an Rhs‐family nuclease effector reveals direct penetration of the gram‐positive cell envelope by a type VI secretion system in Acidovorax citrulli
Authors: Pei Tong‐Tong , Kan Yumin , Wang Zeng‐Hang , Tang Ming‐Xuan , Li Hao , Yan Shuangquan , Cui Yang , Zheng Hao‐Yu , Luo Han , Liang Xiaoye , Dong Tao 
Published: March 2022

AbstractThe type VI secretion system (T6SS) is a double‐tubular nanomachine widely found in gram‐negative bacteria. Its spear‐like Hcp tube is capable of penetrating a neighboring cell for cytosol‐to‐cytosol protein delivery. However, gram‐positive bacteria have been considered impenetrable to such T6SS action. Here we report that the T6SS of a plant pathogen, Acidovorax citrulli (AC), could deliver an Rhs‐family nuclease effector RhsB to kill not only gram‐negative but also gram‐positive bacteria. Using bioinformatic, biochemical, and genetic assays, we systematically identified T6SS‐secreted effectors and determined that RhsB is a crucial antibacterial effector. RhsB contains an N‐terminal PAAR domain, a middle Rhs domain, and an unknown C‐terminal domain. RhsB is subject to self‐cleavage at both its N‐ and C‐terminal domains and its secretion requires the upstream‐encoded chaperone EagT2 and VgrG3. The toxic C‐terminus of RhsB exhibits DNase activities and such toxicity is neutralized by either of the two downstream immunity proteins, RimB1 and RimB2. Deletion of rhsB significantly impairs the ability of killing Bacillus subtilis while ectopic expression of immunity proteins RimB1 or RimB2 confers protection. We demonstrate that the AC T6SS not only can effectively outcompete Escherichia coli and B. subtilis in planta but also is highly potent in killing other bacterial and fungal species. Collectively, these findings highlight the greatly expanded capabilities of T6SS in modulating microbiome compositions in complex environments.
Climate‐induced range shifts of invasive species (Diaphorina citri Kuwayama)
Authors: Aidoo Owusu Fordjour , Souza Philipe Guilherme Corcino , da Silva Ricardo Siqueira , Santana Paulo Antonio , Picanço Marcelo Coutinho , Kyerematen Rosina , Sètamou Mamoudou , Ekesi Sunday , Borgemeister Christian 
Published: March 2022

AbstractBACKGROUNDThe Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is a destructive, invasive species that poses a serious threat to the citrus industry wherever it occurs. The psyllid vectors the phloem‐limited bacteria ‘Candidatus Liberibacter americanus’ and ‘Ca. L. asiaticus’, causal agents of the incurable citrus greening disease or huanglongbing (HLB). It is essential to understand which regions and areas are suitable for colonization by ACP to formulate appropriate policy and preventive measures. Considering its biology and ecology, we used a machine learning algorithm based on the MaxEnt (Maximum Entropy) principle, to predict the potential global distribution of ACP using bioclimatic variables and elevation.RESULTSThe model predictions are consistent with the known distribution of ACP and also highlight the potential occurrence outside its current ecological range, that is, primarily in Africa, Asia and the Americas. The most important abiotic variables driving the global distribution of ACP were annual mean temperature, seasonality of temperature and annual precipitation.CONCLUSIONOur findings highlight the need for international collaboration in slowing the spread of invasive pests like D. citri. © 2022 Society of Chemical Industry.
Quantum Sensing with Extreme Light
Authors: Kutas Mirco , Haase Björn Erik , Riexinger Felix , Hennig Joshua , Bickert Patricia , Pfeiffer Tobias , Bortz Michael , Molter Daniel , von Freymann Georg 
Published: March 2022

AbstractOptical nonlinear conversion processes are ubiquitously applied to scientific as well as industrial tasks. In particular, nonlinear processes are employed to generate radiation in many frequency ranges. In plenty of these nonlinear processes, the generation of paired photons occurs — the so‐called signal and idler photons. Although this type of generation has undergone a tremendous development over the last decades, either the generated signal or the idler radiation has been used experimentally. In contrast, novel quantum‐based measurement principles enable the usage of both partners of the generated photon pairs based on their correlation. These measurement approaches have an enormous potential for future applications, as they allow to transfer information from one spectral range to another. In particular, spectral ranges where photon generation and detection is particularly challenging can benefit from this principle. Above all, these include the extreme frequency ranges, such as on the low‐frequency side the mid to far infrared or even the terahertz spectral range, but also on the high‐frequency side the ultraviolet or X‐ray spectral range. In this review article, theoretical and experimental developments based on correlated biphotons are described specifically for the extreme spectral regions.
Preliminary Results on a Proposed Histopathological Assessment of Predictive Factors for Basal Cell Carcinoma Recurrence after Primary Free Margin Excision
Authors: Jacquet A. , Dormoy V. , Lorenzato M. , Durlach A. 
Published: March 2022

Abstract

Background
Basal cell carcinoma (BCC) incidence is steadily increasing but therapeutic solutions remain limited and present a public health challenge.

Aims
To identify predictive factors of BCC recurrence after primary free margin excision, with automated methods, by evaluating cell proliferation, the Hedgehog pathway activation and primary cilia.

Materials and Methods
This case–control study included 32 patients (16 with recurrence occurring at least 6 months after complete resection, and 16 without recurrence) who underwent surgery for BCC. Formalin-fixed paraffin-embedded cutaneous resections were processed for immunohistochemistry or immunostaining with the following primary antibodies: mouse anti-MCM6, rabbit anti-ARL13B and rabbit anti-GLI1.

Results
BCC recurrence after free margin excision was significantly linked to a higher proliferative index (p &lt; 0.001) and a lower cilia count (p = 0.041) in the primary lesion. No significant differences were observed regarding cilia length (p = 0.39) or GLI1-positive nuclei.

Discussion
The complex interplay between essential signaling pathways, cell proliferation and cilia requires further experimental investigations in the context of BCC recurrence.

Conclusion
A higher proliferative index evaluated with MCM6 antibody could be a useful prognosis marker of BCC risk of recurrence. The lower cilia count in the primary lesion unveiled novel perspectives to understand BCC recurrence molecular mechanisms.
Cell‐Free Extracts from Human Fat Tissue with a Hyaluronan‐Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization
Authors: Xu Guang‐Yu , Xu Shun , Zhang Yu‐Xuan , Yu Zi‐You , Zou Fei , Ma Xiao‐Sheng , Xia Xin‐Lei , Zhang Wen‐Jie , Jiang Jian‐Yuan , Song Jian 
Published: March 2022

AbstractTreatment for spinal cord injuries (SCIs) is often ineffective because SCIs result in a loss of nerve tissue, glial scar formation, local ischemia and secondary inflammation. The current promising strategy for SCI is the combination of bioactive materials and cytokines. Bioactive materials support the injured spinal cord, stabilize the morphology, and avoid excessive inflammatory responses. Fat extract (FE) is a cell‐free liquid component containing a variety of cytokines extracted from human fat tissue using mechanical methods. In this research, a biocompatible HAMC (hyaluronan and methylcellulose) loaded with FE is used to treat a model of spinal cord contusion in mice. The composite not only inhibits death of neuro‐ and vascular cells and leads to the preservation of neural and vascular structure, but also modulates the inflammatory phenotype of macrophages in the locally injured region. Specifically, FE promotes the polarization of macrophages from an inflammatory M1 phenotype to an anti‐inflammatory M2 phenotype. During the screening of the involved pathways, it is corroborated that activation of the STAT6/Arg‐1 signaling pathway is involved in macrophage M2 polarization. In summary, FE is a promising treatment for SCI, as it is easy to obtain, nonimmunogenic, and effective.
Status epilepticus caused by canine distemper virus in a striped hyena (Hyaena hyaena)
Authors: Sarchahi Ali Asghar , Arbabi Mohammad 
Published: March 2022

AbstractA 6‐month‐old free‐living female striped hyena (Hyaena hyaena) was referred to the Veterinary Teaching Hospital of Ferdowsi University of Mashhad, Mashhad, Iran with lethargy and seizures. The general and neurological examinations revealed normal heart rate, polypnoea, abnormal respiratory sounds, decreased consciousness and no postural reactions in limbs. The animal had generalised seizures every 20 minutes, lasting about 1 minute. To control seizures, 10 mg diazepam (0.7 mg/kg) was injected intravenously four times every 2 minutes. With this treatment, the animal's seizures stopped. Based on characteristic clinical signs, lymphopaenia, and positive rapid kit and RT‐PCR results, canine distemper virus (CDV) was confirmed. The animal developed general seizures again 12 hours after referral and died during the seizures. This is the first case report of status epilepticus due to CDV in a striped hyena that did not respond to anticonvulsant therapies and caused the death of the animal.
Non-Abelian Toda field theories from a 4D Chern-Simons theory
Authors: Fukushima Osamu , Sakamoto Jun-ichi , Yoshida Kentaroh 
Published: March 2022

Abstract
We derive non-abelian Toda field theories (NATFTs) from a 4d Chern-Simons (CS) theory with two order defects by employing a certain asymptotic boundary condition. The 4d CS theory is characterized by a meromorphic 1-form ω. We adopt ω with two simple poles and no zeros, and each of the order defects is located at each pole. As a result, an anisotropy parameter β2 can be identified with the distance between the two defects. As examples, we can derive the (complex) sine-Gordon model and the Liouville theory.
Analytic bootstrap in 2D boundary conformal field theory: towards braneworld holography
Authors: Kusuki Yuya 
Published: March 2022

Abstract
Recently, boundary conformal field theories (BCFTs) have attracted much attention in the context of quantum gravity. This is because a BCFT can be dual to gravity coupled to a heat bath CFT, known as the island model. On this background, it would be interesting to explore the duality between the boundary and the braneworld. However, this seems to be a challenging problem. The reason is because although there has been much study of rational BCFTs, there has been comparatively little study of irrational BCFTs, and irrational BCFTs are expected to be the boundary duals of the braneworlds. For this reason, we explore properties of boundary ingredients: the boundary primary spectrum, the boundary-boundary-boundary OPE coefficients and the bulk-boundary OPE coefficients. For this purpose, the conformal bootstrap is extremely useful. This is the first step in providing an understanding of BCFTs in the context of braneworld holography by using the conformal bootstrap. The techniques developed in this paper may be useful for further investigation of irrational BCFTs.
Maintaining a minimally invasive surgical service during a pandemic
Authors: Sivaraj Jayaram , Loukogeorgakis Stavros , Costigan Fiona , Giuliani Stefano , Mullassery Dhanya , Blackburn Simon , Curry Joe , Cross Kate , De Coppi Paolo 
Published: March 2022

Abstract
Purpose
The safety of minimally invasive surgery (MIS) was questioned in the COVID-19 pandemic due to concern regarding disease spread. We continued MIS during the pandemic with appropriate protective measures. This study aims to assess the safety of MIS compared to Open Surgery (OS) in this setting.

Methods
Operations performed during 2020 lockdown were compared with operations from the same time-period in 2019 and 2021. Outcomes reviewed included all complications, respiratory complications, length of stay (LOS) and operating surgeon COVID-19 infections (OSI).

Results
In 2020, MIS comprised 52% of procedures. 29% of MIS 2020 had complications (2019: 24%, 2021: 15%; p = 0.08) vs 47% in OS 2020 (p = 0.04 vs MIS). 8.5% of MIS 2020 had respiratory complications (2019: 7.7%, 2021: 6.9%; p = 0.9) vs 10.5% in OS 2020 (p = 0.8 vs MIS). Median LOS[IQR] for MIS 2020 was 2.5[6] days vs 5[23] days in OS 2020 (p = 0.06). In 2020, 2 patients (1.2%) were COVID-19 positive (MIS: 1, OS: 1) and there were no OSI.

Conclusion
Despite extensive use of MIS during the pandemic, there was no associated increase in respiratory or other complications, and no OSI. Our study suggests that, with appropriate protective measures, MIS can be performed safely despite high levels of COVID-19 in the population.
Representations of the Super Yangians of Types A and C
Authors: Molev Alexander 
Published: March 2022

AbstractWe classify the finite-dimensional irreducible representations of the super Yangian associated with the orthosymplectic Lie superalgebra $\mathfrak {osp}_{2|2n}$

o
s
p

2
|
2
n

. The classification is given in terms of the highest weights and Drinfeld polynomials. We also include an R-matrix construction of the polynomial evaluation modules over the Yangian associated with the Lie superalgebra $\mathfrak {gl}_{m|n}$

g
l

m
|
n

, as an appendix. This is a super-version of the well-known construction for the $\mathfrak {gl}_{n}$

g
l

n

Yangian and it relies on the Schur–Sergeev duality.
Static data-flow analysis for software product lines in C
Authors: Schubert Philipp Dominik , Gazzillo Paul , Patterson Zach , Braha Julian , Schiebel Fabian , Hermann Ben , Wei Shiyi , Bodden Eric 
Published: March 2022

AbstractMany critical codebases are written in C, and most of them use preprocessor directives to encode variability, effectively encoding software product lines. These preprocessor directives, however, challenge any static code analysis. SPLlift, a previously presented approach for analyzing software product lines, is limited to Java programs that use a rather simple feature encoding and to analysis problems with a finite and ideally small domain. Other approaches that allow the analysis of real-world C software product lines use special-purpose analyses, preventing the reuse of existing analysis infrastructures and ignoring the progress made by the static analysis community. This work presents VarAlyzer, a novel static analysis approach for software product lines. VarAlyzer first transforms preprocessor constructs to plain C while preserving their variability and semantics. It then solves any given distributive analysis problem on transformed product lines in a variability-aware manner. VarAlyzer ’s analysis results are annotated with feature constraints that encode in which configurations each result holds. Our experiments with 95 compilation units of OpenSSL show that applying VarAlyzer enables one to conduct inter-procedural, flow-, field- and context-sensitive data-flow analyses on entire product lines for the first time, outperforming the product-based approach for highly-configurable systems.
Modelling Sovereign Credit Ratings: Evaluating the Accuracy and Driving Factors using Machine Learning Techniques
Authors: Overes Bart H. L. , van der Wel Michel 
Published: March 2022

AbstractSovereign credit ratings summarize the creditworthiness of countries. These ratings have a large influence on the economy and the yields at which governments can issue new debt. This paper investigates the use of a multilayer perceptron (MLP), classification and regression trees (CART), support vector machines (SVM), Naïve Bayes (NB), and an ordered logit (OL) model for the prediction of sovereign credit ratings. We show that MLP is best suited for predicting sovereign credit ratings, with a random cross-validated accuracy of 68%, followed by CART (59%), SVM (41%), NB (38%), and OL (33%). Investigation of the determining factors shows that there is some heterogeneity in the important variables across the models. However, the two models with the highest out-of-sample predictive accuracy, MLP and CART, show a lot of similarities in the influential variables, with regulatory quality, and GDP per capita as common important variables. Consistent with economic theory, a higher regulatory quality and/or GDP per capita are associated with a higher credit rating.
Permit Markets with Political and Market Distortions
Authors: Dickson Alex , MacKenzie Ian A. 
Published: March 2022

AbstractThis article investigates cap-and-trade markets in the presence of both political and market distortions. We create a model where dominant firms have the ability to rent seek for a share of pollution permits as well as influence the market equilibrium with their choice of permit exchange because of market power. We derive the equilibrium and show the interaction of these two distortions has consequences for the resulting marginal inefficiency—the extent to which a re-allocation of permits between firms can reduce equilibrium abatement costs. We find that if the regulator is not very responsive to rent seeking then marginal inefficiency reduces relative to the case without rent seeking. When the regulator is very responsive to rent seeking, if dominant rent-seeking firms are all permit buyers (sellers) then marginal inefficiency reduces (increases) relative to the case without rent seeking.
Validity and Reliability of a Fine Motor Assessment for Preschool Children
Authors: Strooband Karel F. B. , Howard Steven J. , Okely Anthony D. , Neilsen-Hewett Cathrine , de Rosnay Marc 
Published: March 2022

AbstractDue to the lack of tools that can be easily used by practitioners, there is a need to develop acceptable embedded ways to assess children’s fine motor skill development within early childhood education and care settings. This study examined the validity and reliability of a brief and ecologically valid fine motor assessment tool for preschool-aged children; the fine motor growth assessment (i.e., FINGA). Children’s fine motor performance on FINGA was compared with widely-used and validated performance-based (Peabody Developmental Motor Scales 2nd edition) and informant-based (Ages and Stages Questionnaire 3rd edition) fine motor assessments. Ninety-one children [mean age (y) ± SD = 4.50y ± 0.68] were assessed within seven early childhood education and care services in New South Wales, Australia. Exploratory factor analyses (EFA) and linear regression analyses showed that FINGA had good internal consistency (EFA of 73%) and age sensitivity (Bstd = 0.69, p < 0.001), and bivariate correlation analyses demonstrated good concurrent validity (rs from 0.69 to 0.84, ps < 0.001) against the two comparison assessments. Because of its brevity and ecological validity, the FINGA tool should be further examined as a routine fine motor skills assessment administered within the context of universal early childhood education and care service provision.