bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic, biochemical, and clinical spectrum of patients with mitochondrial trifunctional protein deficiency identified after the introduction of newborn screening in the Netherlands
Authors: Schwantje Marit , Fuchs Sabine A. , de Boer Lonneke , Bosch Annet M. , Cuppen Inge , Dekkers Eugenie , Derks Terry G. J. , Ferdinandusse Sacha , Ijlst Lodewijk , Houtkooper Riekelt H. , Maase Rose , van der Pol W. Ludo , de Vries Maaike C. , Verschoof‐Puite Rendelien K. , Wanders Ronald J. A. , Williams Monique , Wijburg Frits , Visser Gepke 
Published: April 2022

AbstractLong‐chain 3‐hydroxyacyl‐CoA dehydrogenase deficiency (LCHADD) is included in many newborn screening (NBS) programs. Acylcarnitine‐based NBS for LCHADD not only identifies LCHADD, but also the other deficiencies of the mitochondrial trifunctional protein (MTP), a multi‐enzyme complex involved in long‐chain fatty acid β‐oxidation. Besides LCHAD, MTP harbors two additional enzyme activities: long‐chain enoyl‐CoA hydratase (LCEH) and long‐chain ketoacyl‐CoA thiolase (LCKAT). Deficiency of one or more MTP activities causes generalized MTP deficiency (MTPD), LCHADD, LCEH deficiency (not yet reported), or LCKAT deficiency (LCKATD). To gain insight in the outcomes of MTP‐deficient patients diagnosed after the introduction of NBS for LCHADD in the Netherlands, a retrospective evaluation of genetic, biochemical, and clinical characteristics of MTP‐deficient patients, identified since 2007, was carried out. Thirteen patients were identified: seven with LCHADD, five with MTPD, and one with LCKATD. All LCHADD patients (one missed by NBS, clinical diagnosis) and one MTPD patient (clinical diagnosis) were alive. Four MTPD patients and one LCKATD patient developed cardiomyopathy and died within 1 month and 13 months of life, respectively. Surviving patients did not develop symptomatic hypoglycemia, but experienced reversible cardiomyopathy and rhabdomyolysis. Five LCHADD patients developed subclinical neuropathy and/or retinopathy. In conclusion, patient outcomes were highly variable, stressing the need for accurate classification of and discrimination between the MTP deficiencies to improve insight in the yield of NBS for LCHADD. NBS allowed the prevention of symptomatic hypoglycemia, but current treatment options failed to treat cardiomyopathy and prevent long‐term complications. Moreover, milder patients, who might benefit from NBS, were missed due to normal acylcarnitine profiles.
Impact of different dose reduction criteria for anti‐Xa direct oral anticoagulants on bleeding complications: A single center observational study
Authors: Fukaya Hidehira , Oikawa Jun , Nakamura Hironori , Igarashi Tazuru , Fujiishi Tamami , Ishizue Naruya , Yoshizawa Tomoharu , Satoh Akira , Kishihara Jun , Niwano Shinichi , Ako Junya 
Published: April 2022

AbstractBackgroundEach direct oral anticoagulant (DOAC) has different dose reduction criteria. Here, we evaluated the differences in the doses of three anti‐Xa DOACs and clinical events based on the dose reduction criteria in patients with atrial fibrillation (AF).MethodsConsecutive AF patients prescribed with anti‐Xa DOACs [rivaroxaban (Riva), apixaban (Apix), and edoxaban (Edox)] between April 2011 and May 2016 were retrospectively evaluated. The incidences of thromboembolic and bleeding events were evaluated by the end of December 2020, focusing on the dose proportion.ResultsA total of 786 patients (72 ± 10 years old, 66.9% male) were enrolled in this study [Riva (n = 337), Apix (n = 239), and Edox (n = 210)]. The proportion of reduced dose prescriptions was significantly greater for Edox (79.2%) than Riva (38.7%) or Apix (31.9%). A Kaplan–Meier analysis showed that the incidence of minor bleeding was significantly higher in the Apix than other groups (p < .001), even after propensity score matching. The standard dose of Apix had significantly higher bleeding events than the other DOACs (p < .001). Moreover, 23.2% and 51.6% of the patients with a standard dose of Apix were fulfilled with the dose reduction criteria for Riva and Edox and had more minor bleeding events than the unfulfilled ones (p = .046).ConclusionsThe patients with a standard dose of Apix had a higher incidence of minor bleeding events than the other dosages. A reduced dose of apixaban was not prone to being chosen because of the dose reduction criteria, which may have been associated with a higher minor bleeding rate in patients with Apix.
Changes in heat stress considering temperature, humidity, and wind over East Asia under RCP8.5 and SSP5‐8.5 scenarios
Authors: Juzbašić Ana , Ahn Joong‐Bae , Cha Dong‐Hyun , Chang Eun‐Chul , Min Seung‐Ki 
Published: April 2022

AbstractThe net effective temperature (NET), an index that includes the combined effects of temperature, humidity, and wind, was used along with temperature to assess the impacts of climate change on the heat stress perception in East Asia, one of the regions considered most vulnerable to heat stress. The need for dynamic downscaling has been emphasized because the regional effects of climate change do not follow the global levels linearly. In this study, daily maximums calculated from the 3‐hourly data downscaled by five different regional climate models from four coupled general circulation models participating in Coordinated Regional Climate Downscaling Experiment‐East Asia phase 2 were utilized. To account for the fact human beings acclimate to their environments, 95th percentile of the maximum temperature and maximum NET was used along with the average boreal summer maximum temperatures/NETs. The performance of the models was assessed first, which showed that the models reproduced the current climate well. Future projections revealed an increase in both average and 95th percentile of the maximum temperature and NET over the entire domain for both the RCP8.5 and SSP5‐8.5 scenarios. The increase in heat stress (NET) was slightly larger than the temperature itself, with an increase of up to 7/10°C for temperature and 8/11°C for NET in RCP8.5/SSP5‐8.5, respectively. The overall increases in temperature and NET were projected to be higher in the higher latitudes, while the increase in the frequency of the temperature and NET extremes was predicted to be higher in the already vulnerable regions in the southern part of the domain.
Different responses of soil respiration to climate change in permafrost and non‐permafrost regions of the Tibetan plateau from 1979 to 2018
Authors: Pan Yongjie , Li Xia , Li Suosuo , Li Zhaoguo 
Published: April 2022

AbstractSoil respiration is the primary efflux of carbon dioxide (CO2) in the terrestrial ecosystem. The soil of alpine grassland on the Tibetan Plateau (TP) is rich in soil organic matter, which may release more carbon dioxide as the climate warming. However, due to the limited observations here, there are still deficiencies in understanding the response of soil respiration to climate change, especially the difference between permafrost and non‐permafrost regions. In this paper, we investigate the climatology and trend of soil respiration on the TP from 1979 to 2018, using the Community Land Model version 4.5 (CLM4.5) forced by a suite of high‐resolution atmosphere dataset. Evaluation results show that the land surface model could properly reproduce permafrost extent, and capture the spatial pattern of soil temperature, soil moisture, leaf area index (LAI), and soil respiration. For the whole TP, we find that the spatial pattern for both climatology and trends of soil respiration are correlated with LAI significantly and positively. In addition to the effects of vegetation, precipitation was more correlated with soil respiration than temperature among climatic variables in recent decades. For permafrost and non‐permafrost regions, climate change affects soil respiration in different ways. In permafrost areas, precipitation plays a more important role than temperature. Conversely, in non‐permafrost regions, temperature has a more pronounced effect on soil respiration. The results of this study provide valuable information for predicting greenhouse gas emissions and understanding the carbon cycle on the TP.
Towards a circular economy: The influence of extraction methods on phytosynthesis of metallic nanoparticles and their impact on crop growth and protection
Authors: Rabalao Tshegofatso M. , Ndaba Busiswa , Roopnarain Ashira , Vatsha Banele 
Published: April 2022

AbstractThe increasing food demand owing to aggressive population growth, accompanied by climate change‐related challenges necessitates new, eco‐friendly strategies for sustainable agriculture. Traditional inorganic fertilizers and pesticides for enhanced crop production are usually leached to waterways, leading to detrimental environmental health. Hence, the evolution of smart fertilizers or controlled release of agricultural ingredients is required to reduce and/or minimize leaching and maximize crop productivity. Smart fertilizers include engineered nanoparticles with a large surface area in comparison to their bulk counterparts. This beneficial property can increase soil fertility, crop productivity, and nutrient use efficiency. For a sustainable trajectory, cost‐effective and green synthetic methods for the preparation of such nanoparticles are urgently needed. Thus, research on the use of plant extracts as a green approach in the preparation of nanoparticles has largely increased over the years. Whilst several studies have been published on the green synthesis of nanoparticles, the extraction method in relation to nanoparticle quality has not been extensively explored. This review therefore provides the synthetic protocols of nanoparticles from the plant‐derived extracts and how plant extraction methods influence the morphology of the nanoparticles and thus their application for plant growth and protection.
Computer‐assisted and navigated piezoelectric surgery: A new technology to improve precision and surgical safety in craniomaxillofacial surgery
Authors: Dean Alicia , Heredero‐Jung Susana , Solivera Juan , Sanjuan Alba , Alamillos‐Granados Francisco Jesús 
Published: April 2022

AbstractBackgroundComputer‐assisted navigated piezoelectric surgery (CANPS) is a surgical technique that combines the surgical navigation with a piezoelectric device. This association multiplies the advantages of both technologies, taking the best of each one providing a synergistic association.ObjectiveTo describe and assess the indications, advantages, disadvantages, and complications of this association of surgical techniques.MethodsCANPS was used in 32 patients. The clinical diagnosis was facial trauma, tumors, orthognathic surgeries, temporomandibular joint ankylosis, pathology of the frontal sinus, and alveolar distraction. Nineteen patients were men and 13 were women. Planning software iPlan 3.05 of Brainlab, and Elements of Brainlab were used for planning and the Kolibri and Kurve of Brainlab for surgical navigation. The piezoelectric device used was a “Vercelotti” type in all patients.ResultsCAPNS could be performed successfully in all cases without complications and reduced the surgeon's uncertainty during the osteotomies. There is continuous control of the position of the surgical instrument. The use of the navigated piezoelectric device allowed the surgeon's uncertainty to be reduced during the performance of the osteotomies in depth, in poorly visible areas, with little access or reduced visibility. It also increases the safety of bone resections near important anatomical structures.ConclusionsCANPS combines the advantages of piezoelectric surgery and navigation. CANPS affords real‐time control of the position of the cutting tip and allows semiburied approaches. CANPS allows surgery to be precise, safer, and minimally invasive.
Thermally Activated Delayed Fluorescent (TADF) Mono‐Polymeric OLED with Higher EQE over Its TADF Repeating Unit
Authors: Zhang Jiasen , Wei Qiang , Lyu Lingling , Cao Liang , Zhao Mengyu , Fei Nannan , Wang Tao , Ge Ziyi 
Published: April 2022

AbstractThermally activated delayed fuorescent (TADF) polymers are very suitable for fabricating highly efficient solution‐processed organic light emitting diodes (OLEDs). TADF homopolymers with simple structures are much preferred for preparation, but due to the triplet–triplet annnihilation, most TADF homopolymeric OLED exhibits lower external quantum efficiency (EQE) than its TADF repeating unit. Herein, two series small molecules (CzPhO and PxPhO) and their related mono‐polymers (PCzPhO and PPxPhO) are designed and synthesized for comparison. Among them, phenoxazine‐based materials show TADF properties compared to the carbazole‐based materials, which may be due to the enhanced electron donating ability of phenoxazine unit. In addition, the solution‐processed devices based on phenoxazine‐based polymer PPxPhO give excellent performance compared to their counterparts with the EQEmax of 11.8%.
Discard Mortality of Sea Scallops Placopecten magellanicus Following Capture and Handling in the U.S. Dredge Fishery
Authors: Rudders David B. , Benoît Hugues P. , Knotek Ryan J. , Mandelman John A. , Roman Sally A. , Sulikowski James A. 
Published: April 2022

Abstract
Discard mortality can represent a potentially significant source of uncertainty for both stock assessments and fishery management measures. While the family Pectinidae is considered to be robust to the capture and handling process, understanding species-specific discard mortality rates is critical to characterize both population dynamics and to develop regulatory measures to meet management objectives. The discard mortality rate for the U.S. dredge fishery of sea scallop Placopecten magellanicus was estimated empirically via a retention study aboard industry vessels under commercial conditions. Over 16,000 sea scallops were assessed via a composite index of scallop vitality that consisted of semiqualitative measures of both overt trauma (shell damage) and response to stimuli. Results indicate that overall sea scallop discard mortality was 21% and consistent with the values currently assumed in the stock assessment. Survival mixture models support the utility of a simple metric of physical trauma as an effective predictor of mortality. Exposure time was also identified as a positively correlated factor that was important in describing the discard mortality process. Application of experimental results highlight the need to consider some operational characteristics of the fishery to reduce potential discard mortality.
Impact of material homogeneity assumption on cortical stiffness estimates by MR elastography
Authors: Scott Jonathan M. , Pavuluri KowsalyaDevi , Trzasko Joshua D. , Manduca Armando , Senjem Matthew L. , Huston John , Ehman Richard L. , Murphy Matthew C. 
Published: April 2022

PurposeInversion algorithms used to convert acquired MR elastography wave data into material property estimates often assume that the underlying materials are locally homogeneous. Here we evaluate the impact of that assumption on stiffness estimates in gray‐matter regions of interest in brain MR elastography.MethodsWe describe an updated neural network inversion framework using finite‐difference model–derived data to train convolutional neural network inversion algorithms. Neural network inversions trained on homogeneous simulations (homogeneous learned inversions [HLIs]) or inhomogeneous simulations (inhomogeneous learned inversions [ILIs]) are generated with a variety of kernel sizes. These inversions are evaluated in a brain MR elastography simulation experiment and in vivo in a test–retest repeatability experiment including 10 healthy volunteers.ResultsIn simulation and in vivo, HLI and ILI with small kernels produce similar results. As kernel size increases, the assumption of homogeneity has a larger effect, and HLI and ILI stiffness estimates show larger differences. At each inversion's optimal kernel size in simulation (7 × 7 × 7 for HLI, 11 × 11 × 11 for ILI), ILI is more sensitive to true changes in stiffness in gray‐matter regions of interest in simulation. In vivo, there is no difference in the region‐level repeatability of stiffness estimates between the inversions, although ILI appears to better maintain the stiffness map structure as kernel size increases, while decreasing the spatial variance in stiffness estimates.ConclusionsThis study suggests that inhomogeneous inversions provide small but significant benefits even when large stiffness gradients are absent.
The influence of cystathionine on neurochemical quantification in brain tumor in vivo MR spectroscopy
Authors: Branzoli Francesca , Deelchand Dinesh K. , Liserre Roberto , Poliani Pietro Luigi , Nichelli Lucia , Sanson Marc , Lehéricy Stéphane , Marjańska Małgorzata 
Published: April 2022

PurposeTo evaluate the ability of the PRESS sequence (TE = 97 ms, optimized for 2‐hydroxyglutarate detection) to detect cystathionine in gliomas and the effect of the omission of cystathionine on the quantification of the full neurochemical profile.MethodsTwenty‐three subjects with a glioma were retrospectively included based on the availability of both MEGA‐PRESS and PRESS acquisitions at 3T, and the presence of the cystathionine signal in the edited MR spectrum. In eight subjects, the PRESS acquisition was performed also in normal tissue. Metabolite quantification was performed using LCModel and simulated basis sets. The LCModel analysis for the PRESS data was performed with and without cystathionine.ResultsAll subjects with glioma had detectable cystathionine levels >1 mM with Cramér‐Rao lower bounds (CRLB) <15%. The mean cystathionine concentrations were 3.49 ± 1.17 mM for MEGA‐PRESS and 2.20 ± 0.80 mM for PRESS data. Cystathionine concentrations showed a significant correlation between the two MRS methods (r = 0.58, p = .004), and it was not detectable in normal tissue. Using PRESS, 19 metabolites were quantified with CRLB <50% for more than half of the subjects. The metabolites that were significantly (p < .0028) and mostly affected by the omission of cystathionine were aspartate, betaine, citrate, γ‐aminobutyric acid (GABA), and serine.ConclusionsCystathionine was detectable by PRESS in all the selected gliomas, while it was not detectable in normal tissue. The omission from the spectral analysis of cystathionine led to severe biases in the quantification of other neurochemicals that may play key roles in cancer metabolism.
Carrying weapons at school: Prevalence and associated factors in Colombian high‐school students
Authors: Jiménez‐Villamizar María P. , Campo‐Arias Adalberto , Caballero‐Domínguez Carmen C. 
Published: April 2022

AbstractThe purpose of this study is to identify the prevalence and factors associated with weapon carrying in Colombian high‐school students. A cross‐sectional study was designed to analyze this phenomenon among students aged between 13 and 17 years. A sample of 1462 students participated, 18.46% of which reported having planned suicide, 56.16% reported having started fighting, 22.57% alcohol drinking, 11.63% cannabis smoking, and 20.45% carrying weapons at school. The phenomenon was later associated with the male gender (odds ratio [OR] = 3.49, 95% confidence interval [CI]: 2.59–4.70), alcohol drinking (OR = 2.67, 95% CI: 1.70–4.20), cannabis smoking (OR = 2.39, 95% CI: 1.55–3.70), use of other substances (OR = 2.52, 95% CI: 1.44–4.44), cigarette smoking (OR = 1.83, 95% CI: 1.16–2.88), starting fighting (OR = 2.30, 95% CI: 1.68–3.16), and suicide planning (OR = 2.05, 95% CI: 1.46–2.90). More proximate situational and contextual factors associated with love behavior in adolescents need to be studied.
Registration of ‘S13‐10592C’, a high‐yielding soybean cultivar with resistance to multiple diseases and elevated oil content
Authors: Chen Pengyin , Shannon J. Grover , Scaboo Andrew , Crisel Melissa , Smothers Scotty , Clubb Michael , Selves Stewart , Canella Vieira Caio , Ali MD Liakat , Lee Dongho , Nguyen Henry , Li Zenglu , Mitchum Melissa Goellner , Bond Jason P. , Meinhardt Clinton G. , Usovsky Mariola , Li Shuxian , Mengistu Alemu , Zhang Bo , Mozzoni Leandro Angel , Robbins Robert T. 
Published: April 2022

AbstractThe conventional soybean [Glycine max (L.) Merr.] cultivar ‘S13‐10592C’ (Reg. no. CV‐547, PI 699629) was released by the University of Missouri–Fisher Delta Research Center soybean breeding program in 2019 to meet the high demand for high‐yielding germplasm with elevated oil content. S13‐10592C was derived from a cross of ‘S08‐17361’ × ‘S05‐11482’, which was designed to combine high yield performance, desirable seed traits, and broad disease resistance. The progeny row designated S13‐10592C was visually selected based on yield potential in 2013 and further tested (2014–2018) in 111 environments across nine states, including Missouri and other southern states. S13‐10592C is resistant to stem canker, sudden death syndrome, and charcoal rot. S13‐10592C is a mid‐maturity group IV (relative maturity 4.6) with indeterminate growth habit, white flowers, tawny pubescence, and a tan pod wall at maturity. Seed of S13‐10592C weighs on average 15.7 g 100–1 seeds and consist of 406.3 g kg–1 protein and 234.5 g kg–1 oil on a dry weight basis, which is capable of producing 48.2% of meal protein.
Integrated bioinformatics–cheminformatics approach toward locating pseudo‐potential antiviral marine alkaloids against SARS‐CoV‐2‐Mpro
Authors: Swain Shasank S. , Singh Satya R. , Sahoo Alaka , Panda Pritam Kumar , Hussain Tahziba , Pati Sanghamitra 
Published: April 2022

AbstractThe emergence of the severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) with the most contagious variants, alpha (B.1.1.7), beta (B.1.351), delta (B.1.617.2), and Omicron (B.1.1.529) has continuously added a higher number of morbidity and mortality, globally. The present integrated bioinformatics–cheminformatics approach was employed to locate potent antiviral marine alkaloids that could be used against SARS‐CoV‐2. Initially, 57 antiviral marine alkaloids and two repurposing drugs were selected from an extensive literature review. Then, the putative target enzyme SARS‐CoV‐2 main protease (SARS‐CoV‐2‐Mpro) was retrieved from the protein data bank and carried out a virtual screening‐cum‐molecular docking study with all candidates using PyRx 0.8 and AutoDock 4.2 software. Further, the molecular dynamics (MD) simulation of the two most potential alkaloids and a drug docking complex at 100 ns (with two ligand topology files from PRODRG and ATB server, separately), the molecular mechanics/Poisson‐Boltzmann surface area (MM/PBSA) free energy, and contributions of entropy were investigated. Then, the physicochemical‐toxicity‐pharmacokinetics‐drug‐likeness profiles, the frontier molecular orbitals energies (highest occupied molecular orbital, lowest unoccupied molecular orbital, and ΔE), and structural–activity relationship were assessed and analyzed. Based on binding energy, 8‐hydroxymanzamine (−10.5 kcal/mol) and manzamine A (−10.1 kcal/mol) from all alkaloids with darunavir (−7.9 kcal/mol) and lopinavir (−7.4 kcal/mol) against SARS‐CoV‐2‐Mpro were recorded. The MD simulation (RMSD, RMSF, Rg, H‐bond, MM/PBSA binding energy) illustrated that the 8‐hydroxymanzamine exhibits a static thermodynamic feature than the other two complexes. The predicted physicochemical, toxicity, pharmacokinetics, and drug‐likeness profiles also revealed that the 8‐hydroxymanzamine could be used as a potential lead candidate individually and/or synergistically with darunavir or lopinavir to combat SARS‐CoV‐2 infection after some pharmacological validation.