bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screen-Printed Biocompatible Ultrasensitive Electrochemical Sensor For Real-Time Detection Of Reactive Oxygen Species In Sweat
Authors: Liao Rui , Du Xiaosong , Zhai Zhiqiang , Wang Yang , Li Jun , Zheng Heng , Long Yin , Jiang Yadong 
Published: March 2022

Abstract
Excessive or burst production of reactive oxygen species (ROS) brings oxidative stress that leads to various vital diseases such as cancers, Parkinson’s disease and Ischemia-reperfusion damage, etc. However, traditional biological analysis of ROS based on separate procedure of capturing, labelling, and spectrometrically detecting is complicated and time-consuming, the accuracy of which was severely affected by the short lifespan (microseconds to milliseconds) of ROS, and thus, a fast and effective approach that allows for a real-time detection is highly desired. In this work, we have developed a printable CeO2 − x-CNTs-nanocomposite-based paste and fabricated an integrated flexible ROS sensor (IFRS) by directly printing the paste on a flexible polyethylene terephthalate (PET) substrate as both working electrode and sensing material. This IFRS device demonstrated the fast and real-time response to the hydrogen dioxide in lab as well as to total ROS in sweat of an adult, showing excellent current response to hydrogen oxide in the wide range of 0.01 to 10 mM with a limit of detection (LOD) reaching 1.85 µM. The superior sensitivity of the device to hydrogen oxide was found to be 136.59 \(\text{μA∙}{\text{mM}}^{\text{-1}}\text{∙}{\text{cm}}^{\text{-2}}\), 1.5 to 10 times higher than previously reported sensors. This IFRS device distinguished the instant ROS in the sweat of adult men in vitro, with amperometric response increased 8 times after half an hour strenuous exercise, exhibiting good selectivity and excellent stability together with confirmed high biosafety. Overall, our designed IFRS provides an applicable and translational feasibility toward the simple, rapid and real-time detection of ROS in the near future.
Veterinary Antimicrobials in Cattle Feedlot Environs and Irrigation Conveyances in a High-Intensity Agroecosystem in Southern Alberta, Canada
Authors: Sura Srinivas , Larney Francis J. , Charest Jollin , McAllister Tim A. , Headley John V. , Cessna Allan J. 
Published: March 2022

Abstract
The South Saskatchewan River Basin (SSRB) is considered one of the most intensively farmed regions in Canada, with high densities of livestock and expansive areas of irrigated cropland. We measured concentrations of seven veterinary antimicrobials (VAs) in 114 surface water samples from feedlot environs and 219 samples from irrigation conveyances in the SSRB. Overall, detection frequencies in feedlot environs were 100% for chlortetracycline (CTC) and tetracycline (TC), 94% for monensin (MON), 84% for tylosin (TYL), 72% for lincomycin (LIN), 66% for erythromycin (ERY), and 23% for sulfamethazine (SMZ). For irrigation conveyances, detection frequencies for CTC and TC remained high (94–100%), but dropped to 18% for ERY, 15% for TYL, 10% for MON, and 4% for SMZ. Lincomycin was not detected in irrigation conveyance water. High detection frequencies and median concentrations of VAs in both feedlot environs and irrigation conveyances were associated with high amounts of precipitation. However, an irrigation district (ID) with high livestock density (Lethbridge Northern) did not exhibit higher concentrations of VAs compared to IDs with less livestock, while levels of VAs in irrigation conveyances were less influenced by the degree of surface runoff. The ubiquity of CTC and TC in our study is likely a reflection of its widespread use in intensive livestock operations. Additional investigation is required to link environmental concentrations of VAs with livestock densities, and increase our understanding of potential antimicrobial resistance in high-intensity agroecosystems.
Developing A Third-Degree Burn Model Of Rat Using The Delphi Method
Authors: Chen Zhaoyan , Zeng Ya , Tian Fangyuan 
Published: March 2022

Abstract
Background: The animal experiment plays an essential role in advances on research and treatment of burn. Currently, researchers often rely on personal experience or the literatures to complete the construction of animal disease models, which may lead to the lack of scientific rigor and wide range of animal disease models the reference value. The purposes of this study were aimed to establish a third-degree burn model of rat using a Delphi method in China to provide a reference.Methods: Two rounds of Delphi expert consultation survey was conducted on experts (n=15) in this study, and then used the boundary value method to screen, modify and supplement the indicators. Next, determined the index weight by analytic hierarchy process, and finally the index system of the rat model of third-degree burn was established.Results: Statistical analysis results show that the positive coefficients of the two rounds of expert consultation are 83.33% and 88.67% respectively. The expert authority coefficient values were 0.73 and 0.67 respectively, and the expert coordination coefficient test was P<0.001. According to experts' suggestion, the third-degree burn model of rats with 8 first-degree indexes, 14 second-degree indexes and 46 third-degree indexes was finally established.Conclusions: According to the characteristics and quality requirements of my country's animal models, this study constructs a rat model of third degree burns, which is expected to expressively improve the overall proficiency of burn research quality.
A rapid and high sensitivity RNA detection based on NASBA and G4-ThT fluorescent biosensor
Authors: Jia Guoshuai , Xu Xiaomeng , Guo Zengdan , Hu Xingxing , Pan Qi , Zhu Hanbing , Yi Wang 
Published: March 2022

Abstract
In recent years, various newly emerged and re-emerged RNA viruses have seriously threatened the global public health. There is a pressing need for rapid and reliable nucleic acid–based assays for detecting Viral RNA. Here, we successfully developed a highly sensitive, easy-to-operate G4-ThT-NASBA system to detect RNA that no need for labeled primers and probes. Next, we tested the system for detecting the Classical Swine Fever Virus (CSFV), an RNA virus that causes a highly contagious disease in domestic pigs and wild boar and easily causes huge economic losses. Results showed that the system, integrated the G4-ThT fluorescent biosensor and NASBA(Nuclear acid sequence-based amplification),is capable to detect as little as 2 copies/μL of viral RNA without interfering by other swine viral RNA. Moreover, we were able to detect CSF viral RNA within 2h in serum samples taken from the field in a real-time mode. These findings indicate that the G4-ThT-NASBA system is a rapid, high sensitivity and easy-to-operate technique for RNA detection. The method also has the real-time detection capability which is easily integrated in a highly automated system such as microfluidic chips.
Structure Optimization and Preparation of Power System Grounding Grid Based on Similarity Data Analysis Algorithm
Authors: An Yunzhu , HU Yuanchao , HUANG Tao , Jeon Seunggil , DU Changqing , HU Shangmao 
Published: March 2022

Abstract
The grounding grid of power equipment is the most basic overvoltage protection device for the power system. Before the design and construction of the power grounding grid, the power system generally uses a scaled-down model to verify the characteristics of the grounding grid. As the types and structures of grounding materials become more and more complex, there are one or more similar items in the grounding simulation test that cannot be scaled down. This paper takes the similarity test of a new type of grounding material-flexible graphite composite grounding material as an example. Firstly, the complete similarity theory of grounding simulation test is deduced. Secondly, the source of similar distortion items is analyzed. Then, the distortion error of different similar items is analyzed. Finally, the distortion similarity test of the flexible graphite composite grounding material is taken as an example to verify the test error influence of the distortion similarity item of the grounding simulation. The research results of this paper can provide reference for grounding simulation test, and at the same time, it can be applied to similar tests in other fields.
Distribution of SOCD along different offshore distances in China's fresh-water lake-Chaohu under different habitats
Authors: Yao Xiaojie , Wang Jingjing , Xie Xinyun , Dan Jiang , Xu Xiaoniu 
Published: March 2022

Abstract
Carbon storage in wetland ecosystems is an important part of the carbon cycle of terrestrial ecosystems and provides important ecosystem services. Chaohu Wetland is a typical freshwater lake wetland in China.In this study, soil and plant samples were collected every 500m through three sample lines of different vegetation habitats (estuarine banks, woodlands and shrub beaches) and different offshore distances, revealing the spatial distribution characteristics of soil organic carbon density in Chaohu wetland. The results show that: 1) The overall SOCD of Chaohu Lake wetland is low, the soil carbon density of different habitats is Woodland༞Estuary and riverside༞Shrub and beach, and the SOCD of different offshore distances has no obvious law, and the SOCD content decreases significantly with the deepening of the soil layer; 2) The plant biomass of the Woodland habitat is significantly higher than that of other habitats, and its soil nutrient indicators are also the highest, and the Estuary-riverside habitat has the highest N and P content. Soil and plant nutrients at different offshore distances have no obvious changes; 3) The contents of soil K, Ca, Mg, and N are significantly positively correlated with SOCD, soil bulk density and pH are significantly negatively correlated with SOCD, and vegetation P content is significantly negatively correlated with SOCD. In a comprehensive understanding, the SOCD changes in different spaces of lake wetlands are the result of the combined effects of plant nutrients, biomass, and soil physical and chemical properties. Chaohu wetlands may experience serious degradation. This research provides scientific and technical support for the restoration, protection and scientific management of the Chaohu wetland ecosystem.
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β-Catenin Pathway
Authors: Lone Bilal Ahmad , Siraj Fouzia , Sharma Ira , Ahmad Faiz , Nagar Preeti , Pokharel Yuba Raj 
Published: March 2022

Abstract
Breast cancer is one of the most common cancers with high mortality, highlighting the vital need to identify new therapeutic targets. Here we report that Non-POU Domain-Containing Octamer-Binding Protein (NONO) is overexpressed in breast cancers and validated the interaction between the WW domain of PIN1 and c-terminal Threonine-Proline (thr-pro) motifs of NONO. Interestingly, the interaction of NONO with PIN1 is essential for its stability. Functionally, silencing of NONO inhibits the growth, survival, migration and invasion, epithelial-to-mesenchymal transition (EMT), and stemness of breast cancer cells. Biochemical analysis indicated NONO as a master regulator of the molecules associated with different hallmarks of cancer. Mechanistically, depletion of NONO promotes the expression of PDL1 cell surface protein in breast cancer cells, besides inhibiting the MAPK/β-catenin pathway; and the interaction of NONO with c-Jun and β-catenin proteins displays its role in regulating the oncogenic behavior of cancer cells. Taken together, our results demonstrated an essential role of NONO in the tumorigenicity of breast cancer and could be a potential target for anti-cancerous drugs.
Novel ZrO2-Glycine Nanocomposite As Eco-Friendly High Temperature Corrosion Inhibitor For Mild Steel In Hydrochloric Acid Solution
Authors: Aslam Ruby , Mobin Mohammad , Shoeb M. , Aslam Jeenat 
Published: March 2022

Abstract
We report the green synthesis of novel ZrO2-Glycine nanocomposite (ZrO2-Gly NC) followed by its characterization using X-ray diffraction (XRD), Fourier transforms infrared (FT-IR) spectroscopy, SEM/EDX, and transmission electron microscopy (TEM) techniques. Further, the inhibition effect of the varying concentration of ZrO2-Gly NC on the corrosion of mild steel in 1M HCl was investigated by weight loss and electrochemical measurements at 40-80°C. The percentage inhibition efficacy of NC increased with the increase of concentration and temperature and reached about 81% at 500 ppm at 70°C which decreased slightly (73.5%) at 80 °C. According to the polarization measurements, the investigated ZrO2-Gly NC works as a mixed inhibitor. Also, the adsorption isotherm analysis indicated that the adsorption was spontaneous and followed the Langmuir adsorption isotherm. Furthermore, the contact angle measurement revealed the water-repelling property of the investigated inhibitor. The surface morphological study via SEM-EDS micrograph affirmed the adsorption of ZrO2-Gly NC on the surface of the mild steel.
The Impact of Pulmonary Rehabilitation on the Quality of Life in Schoolchildren with Cystic Fibrosis “Pulmonary Rehabilitation and Quality of Life in CF”
Authors: Hadi Nazanin , farahani azam shirin abadi , nourian manije , nasiri malihe 
Published: March 2022

Abstract
Background
Cystic fibrosis is the most common type of severe chromosomal disease among white people. The disease affects several systems, mainly impacting the lungs. The children with cystic fibrosis have a poor quality of life. Self-management programs such as pulmonary rehabilitation can improve the quality of life. The aim of this study was to investigate the impact of pulmonary rehabilitation on the quality of life in the schoolchildren with cystic fibrosis referring to Tehran Children's Medical Center.
Methods
The present study is a clinical trial in which the samples were randomly placed in two groups of 35, the intervention and the control group. The child demographic information and the Revised Cystic Fibrosis Questionnaire (CFQ-R) were used. In this study, the parent’s version was used for 6 to 11-year-old children and the child’s version for 11 to 13-year-old children as a self-report. In the intervention group, during 6 weeks, 12 sessions were held. Then, the CFQ-R was filled out, immediately and 8 weeks after the intervention. The results were analyzed using SPSS V25, considering a significant level.
Results
According to ANOVA, a significant difference was observed in the mean total scores of the quality of life between the two groups, immediately and 8 weeks after the intervention (P-value < 0.001). In addition, intra-group comparisons show that the impact of time was significant in the intervention group (P-value < 0.001).
Conclusions
Since the difference prior to the intervention and after it was significant, this method can improve the quality of life.
Application to practice
: This technique can be used as a nursing intervention in clinical contexts for children who are sick.
Trial registration number:
IRCT20161024030474N4
Date registration:
2019
Diagnostic and Prognostic Value of Urine Biomarkers among Women with Dysfunctional Voiding
Authors: Kuo Hann-Chorng , Jiang Yuan-Hong , Jhang Jia-Fong , Ho Han-Chen , Hsu Yung-Hsiang 
Published: March 2022

Abstract
The current study aimed to investigate the diagnostic and prognostic value of urine biomarkers among female patients with DV. Urine samples were collected from 43 female patients with DV and 25 controls. Oxidative stress biomarker (8-hydroxy-2-deoxyguanosine [8-OHdG], 8-isoprostane, and [total antioxidant capacity (TAC)]) and inflammatory marker (interleukin-1 beta [IL-1β], IL-2, IL-6, IL-8, tumor necrosis factor alpha, nerve growth factor, and brain-derived neurotrophic factor) levels were analyzed. In total, 26 patients with DV received further treatment with biofeedback pelvic floor muscle exercise or external urethral sphincter botulinum toxin A injections. Patients with DV had significantly higher urine 8-OHdG, IL-1β, IL-8, and brain-derived neurotrophic factor levels than controls. Both urine 8-OHdG and IL-1β levels were positively correlated with clinical symptoms. Patients with DV who had successful treatment outcomes had significantly lower pretreatment urine 8-isoprostane and TAC levels than those with unsuccessful outcomes. The pretreatment urine TAC level was the only independent predictor of successful treatment outcomes (odds ratio: 0.995). Compared with controls, female patients with DV had distinct urine oxidative stress biomarker and inflammatory marker profiles, which also mapped their clinical characteristics and treatment outcomes. These urine analytes might have diagnostic and prognostic values among female patients with DV.
Albumin and infection following a procedure: a Mendelian Randomization study
Authors: Tang Yanchao , Wei Feng , Yu Miao , Zhou Hua , Wang Yongqiang , Cui Zhiyong , Liu Xiaoguang 
Published: March 2022

Abstract
Background
Serum albumin concentration had been proposed to be an important changeable factor increasing the risk of infection following a procedure, and a perioperative albumin supplementation was recommended in some clinical scenarios. However, the results of the published epidemiological observational studies on these hypotheses are still inconclusive. Here, we performed a two-sample Mendelian randomization (MR) analysis to investigate the causal effect of albumin on infection following a procedure using publically available genome-wide association studies (GWAS) summary statistics.
Methods
A series of quality control steps were taken in our analysis to select eligible instrumental single nucleotide polymorphisms (SNPs) which were strongly associated with exposure. To make the conclusions more robust and reliable, we utilized several robust analytical methods (inverse-variance weighted, MR-Egger regression, and Weighted median method) that are based on different assumptions of two-sample MR analysis. The Cochran’s Q test, MR- Pleiotropy RESidual Sum and Outlier (PRESSO) global test, MR-Egger intercept test, and “leave-one-out” sensitivity analysis were performed to evaluate the heterogeneity, horizontal pleiotropy, and stability of these genetic variants on outcome. Outlier variants were removed step-by-step to reduce heterogeneity and the effect of horizontal pleiotropy if identified by the MR-PRESSO outlier test.
Results
Our two-sample MR analysis with one group of exposure GWAS summary statistics and two groups of outcome GWAS summary statistics did not suggest the causal effect of increased/decreased albumin concentration on infection following a procedure. We did not observe the evidence of heterogeneity or directional pleiotropy. There was no potentially influential SNP driving the causal link under “leave-one-out” analysis and the result was stable.
Conclusions
Our MR analysis did not support the causal effect of albumin on infection following a procedure. The result was robust based on several analytical methods. Perioperative albumin supplementation was questionable and should be investigated with more randomized controlled trials.
Intraoperative visualization of a deformed left main stent – left main revascularization is still a topic of discussion
Authors: Müller Philipp Patrick , Heim Christian , Weyand Michael , Harig Frank 
Published: March 2022

Abstract
Background:Coronary artery disease remains the leading cause of mortality in industrialized countries. Treatment options for patients requiring revascularization are constantly re-evaluated by both cardiologists and cardiac surgeons to find an optimal strategy for affected patients. While coronary artery bypass grafting is typically considered first choice for the treatment of left main stenosis, there is a trend towards left main stenting due to a steadily aging population in western countries with a high operative risk and patients with single vessel coronary artery disease affecting the left main artery. Nevertheless left main stenting remains controversial, especially in patients with concomitant indications for open-heart surgery. Case presentation:We want to present a case of a 78-year-old male patient with high-grade aortic stenosis who underwent surgical aortic valve replacement at our heart center due to anatomical contraindications for transcatheter aortic valve replacement. Stenting of the left main coronary artery was performed three years earlier due to single vessel coronary artery disease while moderate aortic valve stenosis was under surveillance at the time of the intervention. Intraoperatively we found the stent to be deformed in the left main coronary artery, covering nearly 25% of the left coronary ostium, consequently cardioplegia for myocardial protection was applied retrograde through the coronary sinus. Conclusion: While left main stenting can be reasonable for a specific population of patients, it should be used cautiously in patients with concomitant indications for open-heart surgery in the near future and a low perioperative risk profile. Coronary artery bypass grafting remains the treatment option of choice for patients with left main stenosis especially in those with a low operative risk and other indications for heart surgery. Clear recommendations for patients with left main stenosis and coexisting moderate aortic valve stenosis are required in future guidelines to improve the long-term outcome for affected patients.
Angiogenic Factor-Based Signature Predicts Prognosis and Immunotherapy Response in Non-Small-Cell Lung Cancer
Authors: Gu Xinpei , Chu Liuxi , Kang Yanlan 
Published: March 2022

Abstract
Non-small-cell lung cancer (NSCLC) is one of the most common malignancies, and specific molecular targets are still lacking. Angiogenesis plays a central regulatory role in the growth and metastasis of malignant tumor. Angiogenic factors (AFs) are involved. Although there are many studies comparing AFs and cancer, a prognostic risk model for AFs and cancer in humans has not been reported in the literature. This study aimed to identify the key AFs closely related to the process of NSCLC development, and four genes have been found, C1QTNF6, SLC2A1, PTX3, FSTL3. Then we constructed a novel prognostic risk model based on these four genes in non-small-cell lung cancer (NSCLC) and fully analyzed the relationship with clinical features, immune infiltration, genomes and predictors. This model had good discrimination and calibration and will perform well for predicting the prognosis of treatment in clinical practice.