bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of senile age on functional outcome and revision rate of unicompartmental knee arthroplasty: a systematic review and meta-analysis
Authors: chen handong , Liu ChangQuan , Ge JunCheng , Zhang Yue , Zhang QiDong , guo wanshou 
Published: July 2022

Abstract
Background
Senile patients have received UKA less than younger patients, and senile age has been regarded as a relative contraindication to UKA. However, as surgical instruments and techniques have improved, the use of UKA in senile patients has also increased.The purpose of this study was to assess the effect of senile age on functional outcomes and cumulative revision rates in patients who underwent primary unicompartmental knee arthroplasty (UKA).
Methods
We performed a systematic search of PubMed, Embase, and the Cochrane Library to identify clinical studies involving the effect of stratified age on outcomes after UKA. The main measures were functional outcome scores including knee society scores (KSS) and Oxford knee scores (OKS) and revision rates. Data was collected by extraction form, and the relative literature was evaluated by using the methodological Index for Nonrandomized Studies (MINORS). Finally, pooled data was analyzed by the use of RevMan5.4 software.
Result
s A total of 8 studies were eligible and included in our study, and it provided a total of 57576 primary UKAs. Patients were divided into two cohort groups based on age classification: elderly age (60–75) and senile age (75–90). In the elderly and senile age groups, there were 37690 and 10942 primary UKAs, respectively. The data analysis showed a statistically significant difference in KSS (objective) and KSS (function) between the senile and elderly age groups (P < 0.05). On the other hand, the OKS decreased significantly in the senile age group (P < 0.05). In addition, the revision rate of UKA in the senile age group was similar or less than that in the elderly age group (P > 0.05).
Conclusion
Senile age is not a vital factor for revision in UKA, and functional outcomes in the senile population are acceptable compared to the younger age groups. Therefore, it is an effective and reliable procedure for relieving pain and improving function in senile patients, and we can infer that disease condition is more vital than age in confirming those who are going to do well postoperatively.
Mediation analysis of erythrocyte lipophilic index on the association between BMI and risk of oral cancer
Authors: Fan Yi , Chen Qing , Wang Yaping , Wang Jing , Li Yanni , Wang Sijie , Weng Yanfeng , Yang Qiujiao , chen Chen , Lin Lisong , Qiu Yu , Chen Fa , Wang Jing , He Baochang , Liu Fengqiong 
Published: July 2022

Abstract
Aims
To explore the relationship between fatty acid lipophilic index (LI) of erythrocyte membrane and oral cancer risk, as well as to evaluate the possibility of LI acting as a mediator of the association between body mass index (BMI) and oral cancer.
Method
In a case-control study from China, 23 fatty acids (FAs) of the erythrocyte membrane were measured using gas chromatography in 380 oral cancer patients and 387 control subjects. The LI was calculated based on FA proportion and FA melting points. The association of BMI and erythrocyte LI with oral cancer risk was analyzed using logistic regression. Mediation effect of the LI on the association between BMI and oral cancer risk was evaluated using mediation analysis.
Results
Among the control group, 46.0% of them were overweight or obese, which was significantly higher than that of oral cancer patients (29.5%). Significant differences of erythrocyte membrane saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs) were observed between the patient and control group. The proportion of C18:1 n-9 from the MUFAs family increased in oral cancer patients (12.67%) compared with control (12.21%). While the total proportion of n-3 PUFAs decreased in oral cancer patients compared with control, with C20:5 n-3 decreased from 0.66–0.47%, and C22:6 n-3 decreased from 5.82–4.86%. The LI was lower in the control participant (M = 27.6, IQR: 27.3–27.9) than that of the oral cancer patients (M = 28.2, IQR: 27.9–28.5). BMI was inversely associated with oral cancer risk with a fully adjusted ORs of 0.59 (95% CI: 0.43–0.83), while LI was positively associated with oral cancer risk with a fully adjusted ORs of 1.99 (95% CI:1.36–2.94). LI explained 7% of the variance in the relationship between BMI and oral cancer risk.
Conclusions
Distribution of the FA profile in erythrocyte membranes differed between the oral cancer patients and control group. The LI derived from the profile of FAs was positively associated with the risk of oral cancer, and the associations between BMI and oral cancer risk could be explained, at least in part, by LI.
Identification of N6-Methyladenosine-Related Gene Based Prognostic Signature to Predict Survival and Potential Therapeutic Drugs in Glioma
Authors: Dong Xingyu , Zhang Yang , Fang Sheng , Li Qiang , Zhang Sunfu , Wu Chunmiao 
Published: July 2022

Abstract
N6-methyladenosine (m6A) modification is one of the most abundant RNA modification methods in the eukaryote. It was reported that abnormal expression of m6A-related genes is closely associated with the tumorigenesis and progression of glioma. However, the role of m6A RNA methylation in the development of glioma is still unclear. In the current study, we defined m6A-related clusters in glioma cohorts from The Cancer Genome Atlas (TCGA) database which exhibited distinct outcomes in OS. From differentially expressed genes between these clusters, m6A-associated prognostic genes were further narrowed down. Eventually, FRA10AC1, GPR85, MARCHF5, and NUAK2 were selected to form the m6Amethylation-based prognostic signature (MMS) of glioma by Cox and LASSO regression analysis. Subsequently, the efficacy and effectiveness of MMS were examined in the training, testing, and whole groups. Kaplan-Meier survival curve indicated high- and low-risk subgroups divided by MMS showed significantly different OS, and the AUCs of MMS reached 0.75 in all groups. In combined analysis with other clinical-pathological factors, MMS was proved as an independent predictive standard of glioma prognosis. In addition, gene enrichment revealed changes in signaling pathways, biological processes and hallmark gene sets between high- and low-risk subgroups. Last but not least, potential therapeutic molecules for the high-risk patient with MMS were subsequently explored. In general, our study provided MMS, a novel molecular panel closely associated with m6A methylation that holds high efficacy in predicting the prognosis of glioma.
Phosphorus deficiencies invoke lipids-like enrich in sweetpotato rhizosphere to stimulate bacterial inositol phosphate metabolism
Authors: Zhu Xiaoya , Wang Jing , Zhang Qiangqiang , Zhao Peng , Liu Ming , Jin Rong , Zhang Aijun , Tang Zhonghou 
Published: July 2022

Abstract
Purpose Rhizosphere is the key part of belowground interaction between plant, microbes and soil. Beneficial interactions between plant roots and rhizosphere microorganisms are pivotal for plant fitness. Given the effort to improve sweetpotato phosphorus (P) efficiency, it is important to understand the adaptive strategies of sweetpotato rhizosphere under limited P availability. The aim of this study was to explore the variation of bacterial community and metabolite profiles of sweetpotato rhizosphere and their interactions under low P stress.Method Rhizosphere samples collected from sweetpotato (Shangshu 19) grown in long-term (41 years) application nitrogen (N) and potassium (K) fertilizers (NK) and N, P and K fertilizers (NPK) soils were analyzed the bacterial community and metabolite profiles by 16S rRNA high-throughput sequencing and liquid chromatography-mass spectrometry (LC-MS), respectively. Conclusion Different fertilization treatments significantly affected the abundances of rare genus in Shangshu 19 rhizosphere. The COG and KEGG enrichment analysis showed the function of bacterial correlated to sugar metabolism and inositol phosphate metabolism were significantly enhanced under low P stress. LC-MS analysis obtained similar results that galactose metabolism and phospholipase D signaling pathway were the top 2 differential metabolic pathways. Our results suggested that the bacteria of Shangshu 19 rhizosphere may use sugars as energy material to respond to low P stress by changing inositol phosphate metabolism. The increases of lipid-like substances played a connecting role, it was produced by sugar metabolism, and then entering mevalonate pathway activate inositol phosphate metabolism. These results have important practical significance for optimizing sweetpotato cultivation system.
Qsar, Molecular Docking, and Adme Studies of Ferulic Acid Derivatives
Authors: Yadav Suchitra , Sharma Aastha , Kumar Mahesh , Narasimhan Balasubramanian 
Published: July 2022

Abstract
Antimicrobial resistance is most often regarded as a serious threat to human health, as well as a major public health concern with numerous and complicated factors leading to its incidence and spread. The ferulic acid derivatives were selected from the reported work by Khatkhar et al., (2015). 2D QSAR identified the nonlinear dependence of biological activity with Log P. In 2D QSAR studies, molecular descriptors include topological parameters like third valence order molecular connectivity index (3χV), valence first-order molecular connectivity index (1χV), Kier's third-order alpha shape index (kα3) and Balaban, lipophilic parameter like log P, electronic parameters like Vamp Lumo and total dipole, govern the antibacterial activity of ferulic acid derivatives. The molecular docking technique predicts binding affinity, drug-receptor interaction, and orientation of drug molecules to the target site, and ADME predicts drug likeliness. Molecular docking studies signify that compounds 18, 15, 21, 32, and 30 have the best docking score against protein transcriptional regulation (PDB ID: 5X14). Based on QSAR, molecular docking results, and binding interaction analysis, ADME studies were employed and show an excellent ADME profile by the Lipinski rule of five. The study suggests that these compounds could be employed as lead structures for advanced research in antimicrobial resistance.
Effect of dual-task training on clinical and biological factors related to sarcopenia in older adults: a quasi-randomized controlled trial
Authors: Sepúlveda-Loyola Walter , Maciel Renata Pires Tricanico , Teixeira Denilson Castro , Araya-Quintanilla Felipe , Junior Rubens Alexandre da Silva , Santos Suhaila Mahmoud Smaili , Rodríguez-Mañas Leocadio , Probst Vanessa Suziane 
Published: July 2022

Abstract
Background
Sarcopenia is a disease associated with different functional and biological factors related to the aging process. Preventing sarcopenia is important because this disease is associated with increased risk of frailty, falls, fractures, hospitalization, and mortality in older adults. Physical exercise has been the most used intervention for the prevention and treatment of sarcopenia. However, most of the exercise interventions have focused on strength training and nutritional supplementation, targeting the intervention for sarcopenia as a problem related only to muscle mass and strength. However, sarcopenia is a multidimensional condition with negative impacts on functional status. Dual-task training is effective in increasing different functional variables such as balance and gait speed. Nevertheless, there are few studies focused on the effect of this intervention on clinical and biological variables related to sarcopenia. For this reason, the objective of this study was to analyze the effects of dual-task training on clinical and biological measurements of sarcopenia in non-sarcopenic older adults.
Methods
48 older adults were included in this study. Fat-free mass, fat mass, calf circunference, handgrip strenght, 5-repetition sit-to stand, 4-meter gait speed, six-minute walking test and oxidative stress blood biomakers were evaluated. Individuals were allocated into one of these two groups: dual-task training (experimental group) and simple-task training (control group) for 12 weeks, 3 times a week, 50 minutes each session. Student's t test, Mann-Whitney test, and ANCOVA, with adjustment for baseline were used. Cohen's effect size was calculated.
Results
Dual-task training increased the fat-free mass and calf circunference, and reduced the fat mass (Cohen's effect size from 0.4 to 1.0; p < 0.05 for all). Both groups showed improvement in 5-repetition sit-to stand, 4-meter gait speed, six-minute walking test (Cohen's effect size from 0.5 to 1.0; p < 0.05 for all). All the antioxidant biomakers (glutathione transferase, catalase, total plasma antioxidant potential and sulfhydryl grouping) increased after dual-task training (Cohen's effect size from 0.4 to 1.0; p < 0.05 for all).
Conclusion
Dual-task training improved muscle mass, muscle strenght and physical performance, which are the clinical measurements of sarcopenia. Additionally, this intervention also increased the antioxidant capacity in healthy older adults.
Efficacy and safety of automated antiseptic lavage and suctioning of oral, oropharyngeal, and subglottic secretions (VapCare) among ventilated patients with COVID-19 developing ventilator-associated events during hospitalization: a prospective randomized open blinded end-point study
Authors: Bhardwaj Vimal , Alva Arjun , Manoj Adarsh 
Published: July 2022

Abstract
BackgroundGlobally, invasive mechanical ventilation is one of the many methods relied on by intensivists to treat patients diagnosed with COVID-19. In this population, substandard oral care and secretion management permit the colonization of pathogens in the oral cavity and aspiration of these microbe-laden secretions is the leading cause of ventilator-associated pneumonia (VAP). This study was conducted to analyze the effects of VapCare, an automated and intelligent secretion clearance and oral hygiene management system for VAP prevention, compared to the manual process.DesignA single-center, prospective, randomized, open-blinded, end-point study was conducted on invasively ventilated patients diagnosed with COVID-19.SettingA tertiary care facility that is National Accreditation Board for Hospitals accredited in Bangalore, India.ParticipantsTwelve adult patients diagnosed with COVID-19 via RT-PCR, antigen, computed tomography scan, or any other approved method intubated for at least 48 hours were recruited from the medical intensive care unit.InterventionPatients were given (n=6) or not given (n=6) the VapCare medical device along with the current standard of care.ResultsSix patients were assigned to the control group (Cx) that received manual suctioning, and six were assigned to the treatment group (Tx) that received VapCare. 3(50%) of the patients in the Cx experienced a ventilator-associated condition (VAC), and 2(33%) of the patients received a confirmed diagnosis of ventilator-associated pneumonia (VAP). There was no incidence of VAC or VAP in the Tx. With an outlier (subject two) excluded, the average cost of antibiotics per patient in the Tx was 32% less at 68,319 INR, while the average cost in the Cx was 100,783 INR. Clearance of secretions was almost 2x in the Tx compared to the Cx. The amount collected from the subglottic region (from where aspirations into the lungs can occur) was lower in the Tx (6.5 ml) when compared to the Cx (7.4 ml), showing that VapCare effectively clears secretions before they reach the subglottic region.ConclusionVapCare can reduce VAP incidence in invasively ventilated patients diagnosed with COVID-19. Antibiotic costs were also reduced by 32% in the Tx compared to the Cx. This study also allows us to hypothesize that automated suctioning can reduce the amount of secretions that can reach the subglottic region and, therefore, potentially reduce the chance of aspiration and, subsequently, VAP.Trial RegistrationClinical Trials Registry – India, CTRI/2021/08/035429. Registered 5 August 2021. Registered Prospectively.
QSAR, Molecular Docking and Molecular Dynamics Simulation Studies for the prediction of Camptothecin derivatives as anticancer agents
Authors: Saini Pradyumn , Kumar Mahesh , Sharma Deepak , Mittal Chirag , Narasimhan Balasubramanian 
Published: July 2022

Abstract
Camptothecin is a cytotoxic pyranoindolizinoquinoline alkaloid that selectively inhibits the nuclear enzyme topoisomerase I. In Present work, the 2D-QSAR, molecular docking and MD simulation studies of anticancer activity of Camptothecin derivative reported by Yang et al.,2019 has been reported. To develop the QSAR model, previously reported IC50 data of 46 CPT derivatives against Hep3B, MCF7, MDA-MB-231, and KB-vin cell lines was extracted. The models suggest that electronic and topological type descriptors are highly correlated with anticancer activity. Molecular docking of CPT derivatives against topoisomerase I revealed that compounds C40, C43, C41 and C32 have best docking score -9.13, -8.96, -8.90, and -8.53 kcal/mol respectively against topoisomerase I and showed binding affinity with residues namely LYS:532, ASN:722, DG:12, DA:113, DC:112, and DT:10. Further MD Simulation study indicated the stability of protein in topo I–C41 complex with an RMSD of 1.7 Å which makes C41 as a lead compound for the development of novel anticancer agent.
The decline of COVID-19 severity and lethality over two years of pandemic
Authors: Marziano Valentina , Guzzetta Giorgio , Menegale Francesco , Sacco Chiara , Petrone Daniele , Urdiales Alberto , Manso Martina del Manso del , Bella Antionino , Fabiani Massimo , Vescio Maria , Riccardo Flavia , Poletti Piero , Manica Mattia , Zardini Agnese , d'Andrea Valeria , Trentini Filippo , Stefanelli Paola , Rezza Giovanni , Palamara Anna Teresa , Brusaferro Silvio , Ajelli Marco , Pezzotti Patrizio , Merler Stefano 
Published: July 2022

Abstract
Undernotification of SARS-CoV-2 infections has been a major obstacle to the tracking of critical quantities such as infection attack rates and the probability of severe and lethal outcomes. We use a model of SARS-CoV-2 transmission and vaccination informed by epidemiological and genomic surveillance data to estimate the number of daily infections occurred in Italy in the first two years of pandemic. We estimate that the attack rate of ancestral lineages, Alpha, and Delta were in a similar range (10–17%, range of 95% CI: 7–23%), while that of Omicron until February 20, 2022, was remarkably higher (51%, 95%CI: 33–70%). The combined effect of vaccination, immunity from natural infection, change in variant features, and improved patient management massively reduced the probabilities of hospitalization, admission to intensive care, and death given infection, with 20 to 40-fold reductions during the period of dominance of Omicron compared to the initial acute phase.
Optimization of tool axis vector for mirror milling of thin-walled parts based on kinematic constraints
Authors: Qian Long , Zhang Liqiang , Gao Qiuge , Yang Jie 
Published: July 2022

Abstract
In the mirror milling of thin-walled parts, the machining path and the change of the tool axis vector will affect the surface quality of the workpiece and the machining efficiency. The machined surface was discretized into point cloud data, and the surface was fitted. Based on the constraints of mirror milling, the tool location points, and machining paths were generated first. Then the tool location points in the space were projected onto the parameter plane to fit the spline curve. What’s more, the position of the corresponding point on the fitting surface was obtained from the tool position point after fitting on the parameter plane and obtaining the tool axis vector. After obtaining the optimized tool position data, the tool axis vector was optimized based on kinematic constraints, precisely, the machine tool rotation axis needs to satisfy the constraints of the maximum angular velocity, maximum angular acceleration, and maximum angular jerk. Therefore, in the process of machining, due to the problems of angular velocity, angular acceleration, and angular jerk at certain tool positions exceeding the limit, feedrate for CNC machines was optimized first. Furthermore, the optimization target was established based on the minimum sum of the motion fluctuations of the rotary axis and the machining time. After that, the optimized machining path and tool axis vector were simulated and tested. Finally, the simulation and experimental results were determined through analysis, which proved the feasibility of the optimized model proposed in this paper. At the same time, the experimental measurement results also showed that the optimized machining path had been significantly improved in both quality and efficiency.
Inheritance of Seed and Flower Color in Flax: A Comprehensive Genetic Study
Authors: Abtahi Mozhgan , Mirlohi Aghafakhr , Ataii Ehsan , Sabzalian Mohammad R. , Jafari Parisa 
Published: July 2022

Abstract
Background: Flower and seed color are two important traits in flax breeding. Flower color is associated with the attraction of insects for pollination, and seed color affects the quality of oilseeds. Eight genetically diverse genotypes of flax with pairwise contrasting seed and flower color were crossed in all directions (56 crosses), and the inheritance mode of seed and flower color was studied in F1, F2 and F3 generations during 2015-2018. Results: Segregation patterns in F1 and F2 generations indicated that seed coat color is primarily determined by maternal genotype, but parents’ genetic constitution finally defines the expression of character. Segregation analysis in the F3 generation indicated that seed coat color is governed by three loci designated as G, D, and Y1. The inheritance patterns of seed color were either monogenic, strongly influenced by variation within a single gene, or epistatic due to interactions between two genes across different genetic backgrounds. Chi-square tests indicated that brown seed color was produced by recessive allele of the Y1 locus and dominant alleles of the G and D loci (GGDDy1y1). The yellow seed color was attributed to the homozygous condition of two recessive genes and one dominant gene (ggDDy1y1 and GGddy1y1) or homozygous condition of three dominant genes (GGDDY1Y1). Flower color was also found to be controlled by three loci assigned as B1, B2, and C′. Blue flower color is governed by dominant alleles at all three loci while, homozygous recessive at any of the three loci will result in a white flower. In the 56 crosses with similar and contrasting flower colors perfect segregating ratios of 3:1, 9:7, 13:3, and 27:37 blue to white flowers in the F2 generation suggested different epistatic interactions between these loci including duplicate-recessive and dominant-recessive. Conclusion: Overall, the findings of this study reveal that the genetic background influences trait inheritance in flax. Our results indicate that the three loci governing the seed color are different from the three controlling the flower color. Still, there is epistatic relationships among the genes for the two traits depending on the genetic background of the parental genotypes.