bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a standardized approach using optimized and contiguous RF applications for electrical isolation of the superior vena cava
Authors: Hilfiker Gabriela , De Becker Benjamin , Haddad Milad El. , O'Neill Louisa , Gillis Kris , Wielandts Jean‐Yves , Tavernier René , De Waroux Jean‐Benoit Le Polain , Duytschaever Mattias , Knecht Sébastien 
Published: November 2022

AbstractThe superior vena cava (SVC) is well described as one of the most common non‐pulmonary vein (PV)—triggers for atrial tachyarrhythmias (ATA). In our study we evaluated a standardized approach for electrical isolation of the SVC from the right atrium using a horseshoe‐shaped lesion set with optimized and contiguous ostial RF lesions. The results are promising, demonstrating a very high rate of acute SVC isolation in a safe and time efficient manner (mostly less than 10 min).
Plant nitrogen availability and crosstalk with phytohormones signallings and their biotechnology breeding application in crops
Authors: Xing Jiapeng , Cao Xiaocong , Zhang Mingcai , Wei Xun , Zhang Juan , Wan Xiangyuan 
Published: November 2022

SummaryNitrogen (N), one of the most important nutrients, limits plant growth and crop yields in sustainable agriculture system, in which phytohormones are known to play essential roles in N availability. Hence, it is not surprising that massive studies about the crosstalk between N and phytohormones have been constantly emerging. In this review, with the intellectual landscape of N and phytohormones crosstalk provided by the bibliometric analysis, we trace the research story of best‐known crosstalk between N and various phytohormones over the last 20 years. Then, we discuss how N regulates various phytohormones biosynthesis and transport in plants. In reverse, we also summarize how phytohormones signallings modulate root system architecture (RSA) in response to N availability. Besides, we expand to outline how phytohormones signallings regulate uptake, transport, and assimilation of N in plants. Further, we conclude advanced biotechnology strategies, explain their application, and provide potential phytohormones‐regulated N use efficiency (NUE) targets in crops. Collectively, this review provides not only a better understanding on the recent progress of crosstalk between N and phytohormones, but also targeted strategies for improvement of NUE to increase crop yields in future biotechnology breeding of crops.
YY1 (Yin‐Yang 1), a transcription factor regulating systemic inflammation, is involved in cognitive impairment of depression
Authors: Lu Jing , Jin Kangyu , Jiao Jianping , Liu Ripeng , Mou Tingting , Chen Bing , Zhang Zhihan , Jiang Chaonan , Zhao Haoyang , Wang Zheng , Zhou Rui , Huang Manli 
Published: November 2022

AimClinical and preclinical studies suggest that alterations in the peripheral and brain immune system are associated with the pathophysiology of depression, also leading to changes in local glucose metabolism in the brain. Here, the authors identified Yin‐Yang 1 (YY1), a transcription factor closely associated with central and peripheral inflammation.MethodsPlasma levels of YY1, interleukin (IL) 6, and IL‐1β in major depressive disorder (MDD) were collected before and after treatment with vortioxetine, and correlation with clinical and cognitive scores was studied. Chronic unpredictable mild stress was treated with vortioxetine. Micropositron emission tomography (microPET) was used to analyze glucose metabolism and mRNA, and the protein level of the YY1–nuclear factor κB (NF‐κB)–IL‐1β inflammatory pathway were measured in related brain regions.ResultsPlasma levels of YY1 and IL‐1β were significantly increased in MDD and decreased after treatment with vortioxetine. Meanwhile, the level of YY1 in plasma was negatively correlated with cognitive functions in patients with MDD and positively correlated with the level of IL‐1β in plasma. Compared with the control group, in chronic unpredictable mild stress rats, (microPET) analysis showed that the decrease of glucose metabolism in the hippocampus, entorhinal cortex, amygdala, striatum, and medial prefrontal cortex was reversed after treatment. mRNA and protein level of related molecular in YY1–NF‐κB–IL‐1β inflammatory pathway decreased in the hippocampus and was reversed by vortioxetine.ConclusionThe current study suggests that the YY1–NF‐κB–IL‐1β inflammatory pathway may play an essential role in both mood changes and cognitive impairment in depression, and may be associated with changes in glucose metabolism in emotion regulation and cognition. These findings provide new evidence for the inflammatory mechanisms of depression.
Exploring the underlying mechanisms of drug‐induced impulse control disorders: a pharmacovigilance‐pharmacodynamic study
Authors: Fusaroli Michele , Giunchi Valentina , Battini Vera , Gringeri Michele , Rimondini Roberto , Menchetti Marco , Radice Sonia , Pozzi Marco , Nobile Maria , Clementi Emilio , De Ponti Fabrizio , Carnovale Carla , Raschi Emanuel , Poluzzi Elisabetta 
Published: November 2022

IntroductionImpulse control disorders (e.g. pathological gambling, hypersexuality) may develop as adverse reactions to drugs. Pathogenetic hypotheses have mainly focused on D3‐receptor agonism, and switching to alternatives with different pharmacologic mechanisms represents a common management strategy. Nonetheless, treatment failure is common and gaining pathophysiological insights is needed.AimWe aimed to identify targets potentially contributing to pathologic impulsivity.MethodWe performed a pharmacovigilance‐pharmacodynamic study on dopamine agonists and antipsychotics using the Food and Drug Administration Adverse Event Reporting System (January 2004–December 2021). We estimated disproportionate reporting using the Bayesian information component. Using online public databases (IUPHAR, ChEMBL, PDSP, DrugBank), we calculated drug occupancies. To identify the targets potentially contributing to impulsivity, we fitted univariate regression models interpolating information components and occupancies within dopamine agonists and antipsychotics. Sensitivity analyses were performed to check for the robustness of the results.ResultsAmong 19 887 reports of impulsivity, 5898 recorded an antipsychotic, and 3100 a dopamine agonist. The more robust signals concerned aripiprazole (N = 3091; median information component [95% confidence interval] = 4.51[4.45–4.55]) and brexpiprazole (229; 4.00[3.78–4.16]) for antipsychotics, pergolide (105; 5.82[5.50–6.06]) and pramipexole (2009; 5.43[5.36–5.48]) for dopamine agonists. Robust, significant positive associations between drug occupancy and impulsivity reporting were found for D3 within dopamine agonists (beta = 1.52; P‐value = 0.047) and 5‐HT1a within antipsychotics (1.92, 0.029).ConclusionOur results supported the role of D3‐receptor agonism in inducing impulsivity in dopamine receptor agonists and identified a potential role of 5‐HT1a receptor agonism in antipsychotics. Investigating these receptors may drive towards a better management of drug‐induced impulsivity.
Liver transplantation for hilar inflammatory myofibroblastic tumor: Case report and review of the literature
Authors: Costa Carolina Magalhães , Neto João Seda , Benavidez Marcel R. , Vincenzi Rodrigo , Roda Karina , Oliveira Caio Márcio V. , Travassos Nathália Porto R. , de Lima Luiz Guilherme C. A. , Pereira Fabio Payão , Fonseca Eduardo A. 
Published: November 2022

AbstractBackgroundInflammatory myofibroblastic tumors (IMTs) of the liver are rare neoplasms. These tumors are difficult to distinguish from other neoplasms by radiological examination, have uncertain evolution, and there is no consensus on the treatment of these lesions. Hilar tumors can involve the portal vein, hepatic artery, bile duct, and spread to the branches of the portal triad, causing obstructive symptoms, occlusive phlebitis, and portal hypertension. Thus, liver transplantation (LT) is an excellent alternative for locally advanced hilar tumors, since it ensures complete tumor resection with free margins.Methods/ResultsWe present a literature review and a case report showing a 3‐year‐old boy with liver IMT invading the hepatic hilum and inferior vena cava, who underwent a successful living donor liver transplantation that required portal vein and vena cava replacement.ConclusionThe incidence of hilar IMTs is low and there is no well‐established standard treatment. Liver transplantation for a hilar tumor with vascular invasion was acceptable in this case because the radically of the surgery was the key for the cure, and because the tumor was benign in nature.
Efficient Synthesis and Evaluation of Novel Sulfonated Dihydropyrimidinthione Derivatives for the Treatment of UV‐B‐Induced Corneal Damage
Authors: Du Enming , Jiang Yueping , Qin Fangyuan , Wang Yange , He Siyu , Wang Gang , Song Zongming , Zhang Junjie , Tao Ye 
Published: November 2022

AbstractUV‐B‐induced corneal damage remains a challenge in clinics, and it is needed to develop novel and effective medicines against UV‐B induced photodamage. 3,4‐Dihydropyrimidine‐2(1H)‐thione derivatives have shown many interesting biological activities, including antibacterial, anti‐inflammatory, antioxidant, etc. In order to find a promising anticorneal photodamage agent, we designed and synthesized two novel sulfonated dihydropyrimidinthione derivatives to evaluate cytoprotective effect against UV‐B‐mediated photodamage. With simple structure, compound 6 possessed good water solubility, photostability and biocompatibility. We demonstrated that 6 exhibited significant cytoprotective effects against UV‐B‐mediated photodamage and the cell viability was up to 93% at 0.2 mg mL−1. The corneal cells were highly sensitive to UV‐B radiation, resulting in the release of inflammatory mediators and DNA damage, which were significantly reversed by 6. Moreover, compound 6 reduced Bax and cleaved Caspase‐3 expressions to suppress UV‐B mediated the intrinsic apoptosis pathway. Our findings suggest that 6 is a promising UV‐B resistant agent with potential to be a promising drug candidate for the treatment of corneal photodamage.
A nonparametric approach for setting safety stock levels
Authors: Saldanha John P. , Price Bradley S. , Thomas Douglas J. 
Published: November 2022

In practice, lead time demand (LTD) can be skewed, multi‐modal or highly variable, and these factors compromise the validity of typical approaches used for setting safety stock levels. Motivated by encountering this problem at our industry partner, we develop an approach for setting safety stock levels using the bootstrap, a widely used statistical procedure. Existing bootstrap approaches for inventory management either operate directly on observed LTD or assume deterministic lead times, permitting direct application of the bootstrap approach for univariate quantile estimation. As LTD is a convolution of multiple random demands over a random lead time, a multivariate bootstrap approach is required. As we demonstrate, when lead times are stochastic, our multivariate approach provides improved safety stock estimates. We develop a multivariate central limit theorem for the bootstrap mean and bootstrap quantile—components of the safety stock calculation—highlighting why the generalization of these bootstrap methods is critical for inventory management. These results provide a theoretical underpinning for the bootstrap estimator of safety stock and permit the construction of confidence intervals for safety stock estimates, allowing decision makers to understand the reliability with which the desired service level will be achieved. Building on our theoretical results, and supported by numerical experiments, we provide insights on the behavior of the bootstrap for various LTD distributions, which our results demonstrate are critical when employing the bootstrap method. Implementation of our approach with our industry partner resulted in an inventory investment reduction of $1.17 million combined with an overall increase in service level. Our approach is general and can be implemented without modification in other settings.
Clarifying the association of social anxiety with cognitive variables: The role of self‐esteem, self‐perception, fears of positive and negative evaluation, and post‐event processing
Authors: Gök B. Göktürk , Yalçınkaya‐Alkar Özden 
Published: November 2022

Theoretical models of social anxiety disorder (SAD) have identified several cognitive factors (fear of negative evaluation [FNE], self‐perception, self‐esteem, and post‐event processing [PEP]) that play a role in the maintenance of the disorder. The current study aimed at testing both the cognitive factors addressed in these different models and the fear of positive evaluation (FPE) in the same construct. A non‐clinical emerging adulthood sample (N = 325) were subjected to structural equation modeling (SEM). The results of the SEM showed that FPE, FNE, and self‐concepts (self‐esteem and self‐perception) were significantly associated with social anxiety. Effect sizes indicated that the variables that most affected social anxiety were FPE, self‐perception, FNE, and self‐esteem, respectively. However, although it was significant in the conceptual model, the direct effect of social anxiety on PEP was not significant in the full model. On the other hand, the present findings add further support to the roles of negative self‐perception and low self‐esteem in social anxiety. Moreover, FPE may not be just delayed/postponed FNE. Considering the effect size of FPE on social anxiety, targeting it when appropriate in treatment may reduce the severity of social anxiety.
Thermal variation across collisional orogens: Insights from the hinterland–foreland transition zone of the Sardinian Variscan belt
Authors: Petroccia Alessandro , Carosi Rodolfo , Montomoli Chiara , Iaccarino Salvatore , Vitale Brovarone Alberto 
Published: November 2022

AbstractUnravelling the architecture and evolution of orogenic wedges are often hampered by the presence of strongly deformed large‐scale nappes made by homogeneous metasedimentary rocks lacking diagnostic metamorphic phases. Involving nappes or tectonic units which occupy most of the chain, reconstructing the thermal architecture of the hinterland–foreland transition zone of collisional belts represents a key point to comprehend the orogenic wedge tectonics. This work provides new thermal data from Raman Spectroscopy on Carbonaceous Material (RSCM) carried out in the low‐grade metamorphic rocks of the hinterland–foreland transition zone in the Variscan belt of Sardinia. The obtained TRSCM highlighted a temperature difference, from ~470–440°C down to ~440–380°C, for the internal and external nappes, respectively. We propose that both nappes experienced syn‐nappe greenschist‐facies metamorphism with different TRSCM, further heating localized along the tectonic boundary in between, that is the Barbagia Thrust (BT), and finally, post‐nappe stacking regional‐scale folding, that deformed the TRSCM spatial distribution and drove the present‐day thermal architecture.
GTP‐stimulated membrane fission by the N‐BAR protein AMPH‐1
Authors: Kustigian Lauren , Gong Xue , Gai Wei , Thongchol Jirapat , Zhang Junjie , Puchalla Jason , Carr Chavela M. , Rye Hays S. 
Published: November 2022

AbstractMembrane‐enclosed transport carriers sort biological molecules between stations in the cell in a dynamic process that is fundamental to the physiology of eukaryotic organisms. While much is known about the formation and release of carriers from specific intracellular membranes, the mechanism of carrier formation from the recycling endosome, a compartment central to cellular signaling, remains to be resolved. In Caenorhabditis elegans, formation of transport carriers from the recycling endosome requires the dynamin‐like, Eps15‐homology domain (EHD) protein, RME‐1, functioning with the Bin/Amphiphysin/Rvs (N‐BAR) domain protein, AMPH‐1. Here we show, using a free‐solution single‐particle technique known as burst analysis spectroscopy (BAS), that AMPH‐1 alone creates small, tubular‐vesicular products from large, unilamellar vesicles by membrane fission. Membrane fission requires the amphipathic H0 helix of AMPH‐1 and is slowed in the presence of RME‐1. Unexpectedly, AMPH‐1‐induced membrane fission is stimulated in the presence of GTP. Furthermore, the GTP‐stimulated membrane fission activity seen for AMPH‐1 is recapitulated by the heterodimeric N‐BAR amphiphysin protein from yeast, Rvs161/167p, strongly suggesting that GTP‐stimulated membrane fission is a general property of this important class of N‐BAR proteins.
The role of RBC antigen transgene integration sites on RBC biology in mice
Authors: Jash Arijita , Hay Ariel M. , Collins J. Baodan , Heo Jinho , Luckey Chance John , Hudson Krystalyn E. , Zimring James C. 
Published: November 2022

AbstractBackgroundTransgenic mice expressing RBC specific antigens are widely used in mechanistic studies of RBC alloimmunization. Existing RBC donor strains have random transgene integration, potentially disrupting host elements that can confound biological interpretation.Study Design and MethodsIntegration site and genomic alterations were characterized by both targeted locus amplification and congenic backcrossing in the five most commonly used RBC alloantigen donor strains (KEL‐K2hi, KEL‐K2med, and KEL‐K2lo, and KEL‐K1). A targeted transgenic approach was developed to allow RBC specific transgene expression from a safe harbor locus (ROSA26). Alloimmune responses were assessed by transfusing alloantigen expressing RBCs into wild‐type recipients and measuring alloantibodies by flow cytometry.Results/FindingsFour of the five analyzed strains had at least one gene disrupted by the transgene integration but none of the disrupted genes are known to be involved in RBC biology. The integration of KEL‐K2med potentially altered the immunological properties of RBCs, although the biological significance of the observed changes is unclear. The ROSA26 targeted approach resulted in a single copy of the transgene that maintains RBC specific expression without random disruption of genomic elements.ConclusionThese findings provide a detailed characterization of genomic disruption by transgene integration found in commonly used RBC donor strains that is relevant to numerous previous publications as well as future studies. With the possible exception of KEL‐K2med, transgene integration is not predicted to affect RBC biology in existing models, and new models can avoid this concern using the described targeted transgenic approach.
Management system of home transfusion in Japan: A nationwide survey in 2019
Authors: Tanaka Asashi , Fujita Hiroshi , Ohashi Kota , Tsujikawa Akihito , Uchiyama Kanami , Ito Tatsuya , Kawashima Koichiro , Kodama Reiko , Mine Takashi , Okuda Makoto , Endoh Teruo , Fukuyoshi Youko , Kitazawa Junichi , Sueoka Eisaburo , Nagai Kazuhiro , Ishida Akaru , Matsuzaki Koji , Kato Yoko , Takanashi Kazuo , Takahashi Koki 
Published: December 2022

AbstractBackground and ObjectivesIn Japan, there are various opinions on the pros and cons of home transfusion because of safety concerns. We hence aimed to elucidate the safety and availability of home transfusion in Japan, which has not been clarified to date.Materials and MethodsClinics throughout Japan that provide home care and have experience in performing blood transfusions were surveyed. The analysis period was February to December 2019. Basic information about the clinics, their collaboration system with core hospitals, storage method of red blood cells (RBCs) and the system for the management of patient information regarding transfusion reactions were investigated.ResultsDetailed information was obtained regarding the implementation of home transfusions by 51 clinics. The proportion of home care clinics performing home transfusions was 17.6%, and they were more frequently performed in urban regions. Approximately half of the clinics collaborated with a core hospital for emergency responses to transfusion reactions. At 84% of the clinics, RBC units were stored in refrigerators that were not exclusively allocated to blood storage. Nurses and family members were involved as patient attendants in 83% and 77% of the home transfusions, respectively. No serious transfusion reactions were reported among the 150 patients in 2019, nor the 623 patients up to 2018.ConclusionFrom data on its availability and safety, home transfusions are considered to be in the developing phase in Japan. Increased cooperation between hospitals and clinics is crucial towards improving the home transfusion system in Japan in the future.
Patient informed consent for a clinical trial of gene‐edited pig kidney transplantation: A representative consent form
Authors: Cooper David K. C. 
Published: November 2022

AbstractWhen clinical trials of gene‐edited pig organ transplantation are initiated, the consent form that the patient is requested to sign will be an important document. Consent to receive a pig xenograft will have significant differences when compared with the requirements of most experimental clinical procedures. We here suggest a consent form for pig kidney transplantation that addresses the major points that will be required and hope it will provide a basis for discussion and future modification, if necessary. There is purposely some repetition in the document, but we believe this is necessary to ensure that the patient has a clear understanding of what he/she is consenting to.
An Analytical Stress–Deflection Model for Fixed-Clamped Flexures Using a Pseudo-Rigid-Body Approach
Authors: Huxman Connor , Butler Jared 
Published: December 2022

Abstract
Fixed-clamped flexures are one common component of compliant mechanisms which remain difficult to design due to their unique force– and stress–deflection profiles. In this work, an analytical stress-deflection model for fixed-clamped flexures is proposed that utilizes a modified pseudo-rigid-body model. Proof-of-concept mechanical testing and finite element analysis demonstrate that the model can predict forces and stresses within 3.5% for a range of steel flexure topologies. Special analysis is carried out on the characteristic radius factor, a parameter to which model accuracy is particularly sensitive. For slender flexures or large deflection scenarios, a dynamic characteristic radius factor is required to capture the resulting nonlinear axial strain. By evaluating the effects of loading, geometry, and material properties, an analytical equation that can predict an optimal value is proposed. When integrated into our model, this equation for an appropriate characteristic radius factor can predict the optimal parameter value within 0.45 ± 0.47%, resulting in average model error of 3.45 ± 2.09% across a large range of flexure thicknesses and deflections. The distinct combination of axial and bending stresses experienced in fixed-clamped flexures has made mechanisms that use these members challenging to design. This work provides a model that designers, engineers, and researchers can draw from to understand stress profiles present in these flexible members.