bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lentilactobacillus buchneri domination during the fermentation of Japanese traditional fermented fish (funazushi)
Authors: Tanabe Koichi , Monguchi Masaki , Inoue Ryoga , Zamami Rio , Nakanishi Ryo , Manabe Ayano , Oe Kaho , Komatsuzaki Noriko , Shima Jun 
Published: July 2022

AbstractFunazushi is a Japanese traditional fermented fish made with boiled rice without the addition of microbial starter cultures. Isolates from various commercial funazushi products, as identified by 16S rDNA sequences, suggested that Lentilactobacillus buchneri strains are major lactic acid bacteria. Based on an analysis of the putative CRISPR (clustered regularly interspaced short palindromic repeat) region, the genetic diversity of L. buchneri strains was examined. The data suggested that the diversity of L. buchneri strains depended on the factories at which funazushi was produced. An analysis of samples during fermentation indicated that the transition of microbes occurred, and L. buchneri was the dominant species. To determine the factors associated with domination, bacteriocin production and environmental stress tolerance, including NaCl and organic acid (lactate and acetate) tolerance, were evaluated. L. buchneri isolates did not produce bacteriocin. Although the isolates did not exhibit NaCl tolerance, they displayed higher lactate tolerance than other lactic acid bacteria isolated during funazushi fermentation. Based on reports that L. buchneri can convert lactate to acetate, the previous and present results suggested that lactate tolerance and lactate conversion in L. buchneri could explain its domination in funazushi. Our study presented a model for the domination mechanisms of specific microbes in fermented foods by spontaneous fermentation.
Identification of the promising accessions of spistan (Cordia myxa Roxb.) using morphological and fruit‐related traits
Authors: Mirheidari Farhad , Khadivi Ali , Moradi Younes , Paryan Simin 
Published: July 2022

AbstractSpistan (Cordia myxa Roxb.) is a potentially underutilized fruit plant in arid and semi‐arid regions. It has long been associated with health and nutrition. Morphological diversity of 75 accessions of this species was evaluated. The accessions studied showed significant differences in terms of the characters measured. Ripening date varied from late May to mid‐July. Fruit color was yellow‐cream in 54, light orange in 8, and orange in 13 accessions. The range of fruit weight was 0.71–11.83 g with an average of 3.12, while fruit flesh thickness ranged from 0.63 to 7.86 mm with an average of 2.55, and fruit jelly part thickness varied between 1.34 and 6.40 mm with an average of 2.75. Principal component analysis could describe the evaluated traits as the nine main components that were able to justify 79.04% of total variance. Hierarchical clustering showed that the accessions were placed into two main clusters using the measured traits data, exhibiting a wide range of variability. Based on the traits related to selection of the ideal genotype, such as big fruit size, high fruit flesh thickness with high yield, and longer harvesting period, 11 accessions, including Jangal‐4, Jangal‐9, Rask‐7, Jangal‐5, Jangal‐6, Jangal‐11, Rask‐1, Jangal‐1, Rask‐4, Rask‐5, and Rask‐2, were superior. It is recommended to use the best accessions selected in breeding programs. The commercial orchards of those best accessions should be extensively constructed to take advantage of the high yield of C. myxa as a crop and its medicinal properties.
Educational attainment polygenic scores, socioeconomic factors, and cortical structure in children and adolescents
Authors: Merz Emily C. , Strack Jordan , Hurtado Hailee , Vainik Uku , Thomas Michael , Evans Alan , Khundrakpam Budhachandra 
Published: July 2022

AbstractGenome‐wide polygenic scores for educational attainment (PGS‐EA) and socioeconomic factors, which are correlated with each other, have been consistently associated with academic achievement and general cognitive ability in children and adolescents. Yet, the independent associations of PGS‐EA and socioeconomic factors with specific underlying factors at the neural and neurocognitive levels are not well understood. The main goals of this study were to examine the unique contributions of PGS‐EA and parental education to cortical structure and neurocognitive skills in children and adolescents, and the associations among PGS‐EA, cortical structure, and neurocognitive skills. Participants were typically developing 3‐ to 21‐year‐olds (53% male; N = 391). High‐resolution, T1‐weighted magnetic resonance imaging data were acquired, and cortical thickness (CT) and surface area (SA) were measured. PGS‐EA were computed based on the EA3 genome‐wide association study of educational attainment. Participants completed executive function, vocabulary, and episodic memory tasks. Higher PGS‐EA and parental education were independently and significantly associated with greater total SA and vocabulary. Higher PGS‐EA was significantly associated with greater SA in the left medial orbitofrontal gyrus and inferior frontal gyrus, which was associated with higher executive function. Higher parental education was significantly associated with greater SA in the left parahippocampal gyrus after accounting for PGS‐EA and total brain volume. These findings suggest that education‐linked genetics may influence SA in frontal regions, leading to variability in executive function. Associations of parental education with cortical structure in children and adolescents remained significant after controlling for PGS‐EA, a source of genetic confounding.
In vitro evaluation of the inhalation toxicity of the cosmetic ingredient aluminum chlorohydrate
Authors: Thá Emanoela Lundgren , Gagosian Viviana Stephanie Costa , Canavez Andrezza Di Pietro Micali , Schuck Desiree Cigaran , Brohem Carla Abdo , Gradia Daniela Fiori , de Freitas Rilton Alves , Prado Karin Braun , Cestari Marta Margarete , Lorencini Márcio , Leme Daniela Morais 
Published: July 2022

AbstractAluminum chlorohydrate (ACH) is a major aerosol component frequently used as the active ingredient in antiperspirants, and in vivo studies have raised a concern about its inhalation toxicity. Still, few studies have addressed its effects on the human respiratory tract. Therefore, we developed a study on ACH inhalation toxicity using an in vitro human alveolar cell model (A549 cells) with molecular and cellular markers of oxidative stress, immunotoxicity, and epigenetic changes. The chemical characterization of ACH suspensions indicated particle instability and aggregation; however, side‐scatter analysis demonstrated significant particle uptake in cells exposed to ACH. Exposure of A549 cells to non‐cytotoxic concentrations of ACH (0.25, 0.5, and 1 mg/ml) showed that ACH induced reactive oxygen species. Moreover, ACH upregulated TNF, IL6, IL8, and IL1A genes, but not the lncRNAs NEAT1 and MALAT1. Finally, no alterations on the global DNA methylation pattern (5‐methylcytosine and 5‐hydroxymethylcytosine) or the phosphorylation of histone H2AX (γ‐H2AX) were observed. Our data suggest that ACH may induce oxidative stress and inflammation on alveolar cells, and A549 cells may be useful to identify cellular and molecular events that may be associated with adverse effects on the lungs. Still, further research is needed to ensure the inhalation safety of ACH.
Therapeutic effects of statins on osteoarthritis: A review
Authors: Saberianpour Shirin , Abolbashari Samaneh , Modaghegh Mohamad H. S. , Karimian Maryam S. , Eid Ali H. , Sathyapalan Thozhukat , Sahebkar Amirhossein 
Published: July 2022

AbstractOsteoarthritis (OA) is a progressive joint disease. The etiology of OA is considered to be multifactorial. Currently, there is no definitive treatment for OA, and the existing treatments are not very effective. Hypercholesterolemia is considered a novel risk factor for the development of OA. Statins act as a competitive inhibitor of the β‐hydroxy β‐methylglutaryl‐CoA (HMG‐CoA) reductase and are widely used to manage hypercholesterolemia. Inhibition of HMG‐CoA reductase results in reduced synthesis of a metabolite named mevalonate, thereby reducing cholesterol biosynthesis in subsequent steps. By this mechanism, statins such as atorvastatin and simvastatin could potentially have a preventive impact on joint cartilage experiencing osteoarthritic deterioration by reducing serum cholesterol levels. Atorvastatin can protect cartilage degradation following interleukin‐1β‐stimulation. Atorvastatin stimulates the STAT1‐caspase‐3 signaling pathway that was shown to be responsible for its anti‐inflammatory effects on the knee joint. Simvastatin had chondroprotective effects on OA in vitro by reducing matrix metalloproteinases expression patterns. In this study, we tried to review the therapeutic effects of statins on OA.
Dilemmas when talking about Huntington's disease: A qualitative study of offspring and caregiver experiences in Norway
Authors: Kjoelaas Siri , Jensen Tine K. , Feragen Kristin B. 
Published: July 2022

AbstractResearch provides a compelling list of reasons why offspring should be included in honest conversations about disease when the disease affects their caregivers. Despite this, we lack in‐depth knowledge about how families affected by the severe and complex genetic condition Huntington's disease (HD) experience talking about the many aspects of how this disease affects their lives. This study aimed to provide an in‐depth exploration of how offspring with a caregiver with HD and caregivers with a partner with HD experienced talking about disease throughout childhood. Thematic analysis was conducted with semistructured interviews of both caregivers (n = 14) and offspring (n = 36) from families affected by HD, reflecting both current and past experiences. In addition to highlighting the many needs offspring have for knowledge and conversation about the disease with their caregivers, our findings also show that a variety of dilemmas can follow these conversations, including when to talk, what to say, how often HD should be talked about on a day‐to‐day basis, and whether to share disease‐related information with others outside the family. The findings show the complexity of talking with offspring about HD. A difficult task for both offspring and caregivers seemed to be finding out how to balance the many dilemmas that arise in conversations and how to use dialogue to best help offspring adapt and cope with the many challenges that can come with HD. The findings can assist health care professionals, such as genetic counselors, prepare, and guide families affected by HD in the many and complex conversations that arise about the disease, in turn helping offspring adjust and cope with their current lives or future lives affected by HD.
Clinical characteristics and illness course based on pathogen among children with respiratory illness presenting to an emergency department
Authors: Windsor W. Jon , Lamb Molly M. , Dominguez Samuel R. , Mistry Rakesh D. , Rao Suchitra 
Published: July 2022

AbstractUpper respiratory illnesses due to viruses are the most common reason for pediatric emergency department (ED) visits in the United States. We explored the clinical characteristics, hospitalization risk, and symptom duration of children in an ED setting by respiratory pathogen including coinfections. A retrospective analysis was conducted from a randomized controlled trial evaluating a rapid molecular pathogen panel among 931 children 1 month to 18 years of age with acute respiratory illness. We assessed hospitalization risk by pathogen using multivariable Poisson regression with robust variance. Symptom duration was assessed using multivariable Cox proportional hazards models. Among 931 children, 702 (75%) were aged 0–5 years and 797 (85%) tested positive for a respiratory pathogen. Children with respiratory syncytial virus (RSV), human metapneumovirus (hMPV), and human rhinovirus/enterovirus (HRV/EV) had higher hospitalization risk compared with influenza (adjusted risk ratio [aRR]: 2.95, 95% confidence interval [CI]: 1.17–7.45; 3.56, 95% CI: 1.05–12.02; aRR: 2.58, 95% CI: 1.05–6.35, respectively). Children with RSV, parainfluenza and atypical bacterial pathogens had longer illness duration compared with influenza (adjusted hazards ratio [aHR]: 2.16 95% CI: 1.41–3.29; aHR: 1.67, 95% CI:1.06–2.64; aHR: 2.60 95% CI: 1.30–5.19, respectively). Children with RSV, hMPV, and atypical bacterial pathogens had higher illness severity and duration compared with other respiratory pathogens. Coinfection was not associated with increased illness severity.
Orientation Toward Intrinsic Motivation Mediates the Relationship Between Metacognition and Creativity
Authors: Urban Marek , Urban Kamila 
Published: July 2022

ABSTRACTMetacognition and motivation are considered key facets of self‐regulation in various contexts. Recent studies identified a link between metacognition and creative performance, with metacognitively aware students performing more creatively and exhibiting higher levels of intrinsic and identified extrinsic motivation. The present study aims to examine the relationship between metacognition, orientation toward intrinsic or extrinsic motivation, and creative performance. One hundred nineteen university students completed the Metacognitive Awareness Inventory (MAI) and Scale of Intrinsic versus Extrinsic Orientation in the Classroom and performed four verbal creativity tasks (product improvement task, consequences task, and two unusual uses tasks). The partial correlation network showed that all the creativity tasks were uniquely related to at least one facet of metacognition, and that the most complex task (product improvement task) was linked to both metacognitive knowledge and regulation. Furthermore, the structural equation model indicated that orientation toward intrinsic motivation mediated the relationship between metacognition and creative performance, explaining 16% of the variance in creative performance.
Dietary flavonoids: a novel strategy for the amelioration of cognitive impairment through intestinal microbiota
Authors: Zhang Yuting , Cheng Lu , Liu Yanan , Zhan Shengnan , Wu Zufang , Luo Songmei , Zhang Xin 
Published: July 2022

AbstractThe chances of people suffering from cognitive impairments increase gradually with age. Diet and lifestyle are closely related to the occurrence and development of cognitive function. Dietary flavonoid supplementation has been shown to be one of the protective factors against cognitive decline. Flavonoids belong to a class of polyphenols that have been proposed for the treatment of cognitive decline. Recent evidence has shown that intestinal flora in the human body can interact with flavonoids. Intestinal microbiota can modify the chemical structure of flavonoids, producing new metabolites, the pharmacological activities of which may be different from those of the parent; meanwhile, flavonoids and their metabolites can, in turn, regulate the composition and structure of intestinal flora. Notably, intestinal flora affect host nervous system activity through the gut‐brain axis, ultimately causing changes in cognitive function. This review therefore summarizes the interaction of dietary flavonoids and intestinal flora, and their protective effect against cognitive decline through the gut‐brain axis, indicating that dietary flavonoids may ameliorate cognitive impairment through their interaction with intestinal microbiota. © 2022 Society of Chemical Industry.
Editorial bias in top‐tier education journals: Factors influencing publishable scholarship in China
Authors: Jiang Xiaohua , Shi Yabing 
Published: July 2022

AbstractThough not rare in the gatekeeping practices of journals, editorial bias in China has received scant scholarly attention. Drawing on in‐depth interviews with 15 academics and 10 editors in the field of education and collecting data on more than 1,522 scholarly articles published by the Chinese journal Educational Research, we assessed the causes and consequences of editorial bias in a subset of the education journals covered by the Chinese Social Sciences Citation Index (CSSCI). Our findings indicate that the editorial biases revealed in some top‐tier Chinese education journals were associated mainly with academic fame, rank, and affiliation as well as personal relationships described by the term guanxi. The causes of these biases include the desire to secure the status of CSSCI‐listed journals, short‐staffed editorial teams, and an inadequate peer‐review system. Further, we found these biases to be associated with three particular negative consequences: slowing the professional development of young academics, allowing the publication of mediocre articles and compromising the Chinese academic environment. We conclude that journal editors, reviewers and academics must work together to safeguard a rigorous and constructive scholarly publishing system in China.
Guided Bone Regeneration with a Nitric‐Oxide Releasing Polymer Inducing Angiogenesis and Osteogenesis in Critical‐Sized Bone Defects
Authors: Won Jong‐Eun , Kim Won Jong , Shim Ji Suk , Ryu Jae Jun 
Published: July 2022

AbstractSynthetic scaffolds, as bone grafts, provide a favorable environment for the repair and growth of new bone tissue at defect sites. However, the lack of angio‐ and osteo‐induction limits the usefulness of artificial scaffolds for bone regeneration. Nitric oxide (NO) performs essential roles in healing processes, such as regulating inflammation and addressing incomplete revascularization. In this study, a polymer capable of controlled NO release is developed to promote the osteogenic capacity in artificial scaffolds. The biological efficiency of the NO compound is assessed by its effect on pre‐osteoblasts and macrophages in vitro and the extent of vascularization and bone formation in the calvaria defect model in vivo. The compound does not inhibit cell adhesion or proliferation. NO treatment significantly increases both alkaline phosphatase activity and mineralization in pre‐osteoblasts. Macrophages treated with NO secrete high levels of anti‐inflammatory factors and adopt the pro‐regenerative phenotype. In the critical‐sized defect model, the collagen scaffold containing the NO compound enhances neovascularization and bone formation. The developed NO‐releasing system promotes osteogenesis and regeneration of damaged bone tissue. As the multiple functions of NO involve macrophage modulation and angiogenesis, such release systems may be valuable for guiding bone regeneration in critical‐sized defects.
3D Printing GelMA/PVA Interpenetrating Polymer Networks Scaffolds Mediated with CuO Nanoparticles for Angiogenesis
Authors: Hu Qingxi , Lu Runsheng , Liu Suihong , Liu Yakui , Gu Yan , Zhang Haiguang 
Published: July 2022

AbstractBiocompatible hydrogels have been considered one of the most well‐known and promising in various materials used in the fabrication of tissue‐engineering scaffolds. Although considerable progress has been made in recent decades, many limitations remain, such as poor mechanical and degradation properties of biomaterials. In addition, vascularization of tissue‐engineering scaffold is an enduring challenge, which limited the fabrication and application of scaffold with clinically relevant dimension. To cover these challenges, in this work, a novel nanocomposite interpenetrating polymer networks (IPN) hydrogel scaffold consists of methacrylated gelatin (GelMA), poly(vinyl alcohol) (PVA), and copper oxide nanoparticles (CuONPs) is fabricated by extrusion‐based 3D printing. A series of physiochemical and biological characterizations of the nanocomposite GelMA/PVA scaffolds are performed. Results showed that the mechanical and degradation properties of the nanocomposite GelMA/PVA scaffolds are obviously improved compared to GelMA scaffolds with single network. In vitro cell experiments and chick embryo angiogenesis (CEA) assay confirmed good cytocompatibility of the fabricated scaffold and its potential to promote cell migration and angiogenesis. In conclusion, altogether the results demonstrated that GelMA/PVA IPN scaffolds modified with CuONPs have great potential for fabrication of volumetric scaffolds and promote angiogenesis during tissue growth and repair.
Electrically Controlled Click‐Chemistry for Assembly of Bioactive Hydrogels on Diverse Micro‐ and Flexible Electrodes
Authors: Da Silva Aruã Clayton , Akbar Teuku Fawzul , Paterson Thomas Edward , Werner Carsten , Tondera Christoph , Minev Ivan Rusev 
Published: July 2022

AbstractThe seamless integration of electronics with living matter requires advanced materials with programmable biological and engineering properties. Here electrochemical methods to assemble semi‐synthetic hydrogels directly on electronically conductive surfaces are explored. Hydrogels consisting of poly (ethylene glycol) (PEG) and heparin building blocks are polymerized by spatially controlling the click reaction between their thiol and maleimide moieties. The gels are grown as conformal coatings or 2D patterns on ITO, gold, and PtIr. This study demonstrates that such coatings significantly influence the electrochemical properties of the metal‐electrolyte interface, likely due to space charge effects in the gels. Further a promising route toward engineering and electrically addressable extracellular matrices by printing arrays of gels with binary cell adhesiveness on flexible conductive surfaces is highlighted.
Graphical methods for understanding changes in states: Understanding medication use pathways
Authors: Wise Elizabeth A. , Adams Roy J. , Lyketsos Constantine G. , Leoutsakos Jeannie‐Marie 
Published: July 2022

AbstractObjectivesAs epidemiological studies become longer and larger, the field needs novel graphical methods to visualize complex longitudinal data. The aim of this study was to present the Slinkyplot, a longitudinal crosstabulation, to illustrate patterns of antidepressant use in a large prospective cohort of older adults with mild cognitive impairment.MethodsData from the National Alzheimer's Coordinating Center are used to track switches between different states and types of antidepressant use. A Slinkyplot is populated with rows representing the state of medication use at each timepoint and columns representing the state at each subsequent visit.ResultsThe constructed Slinkyplots display the common practice of switching on and off different antidepressants over time, with citalopram, sertraline, and bupropion most commonly used followed by switching to another SSRI or SNRI as second‐line treatment.ConclusionsSlinkyplots are an innovative graphical means of visualizing complex patterns of transitions between different states over time for large longitudinal studies.
Measuring and estimating insulin resistance in clinical and research settings
Authors: Gastaldelli Amalia 
Published: July 2022

AbstractThe article discusses how to measure insulin resistance in muscle, liver, and adipose tissue in human participants. The most frequently used methodologies to evaluate insulin resistance are described in detail starting from the gold standard, that is, the euglycemic hyperinsulinemic clamp, to the intravenous glucose tolerance test, surrogate indices based on fasting measurements, or dynamic tests (such as oral glucose or mixed meal tolerance tests). The accuracy, precision, and reproducibility of the tests as well as cutoff values are reported.