bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elaborated EECM parameter identification reflecting adaptive weight for achieving a high‐accuracy SOC estimator
Authors: Kim Gunwoo , Park Jinhyung , Sin Seunghwa , Kim Jonghoon , Baek Jongbok 
Published: July 2022

AbstractIn this paper, a combined method is proposed to improve the accuracy of an electrical equivalent circuit model (EECM) and state‐of‐charge (SOC) estimation in situations where a lithium‐ion battery is frequently charged and discharged. To calculate the terminal voltage in the dynamic region, where charging and discharging are frequently repeated at 0.1 s intervals, a specific region is determined and historical weight is selected, based on the amount of charge in that region, to reflect the model parameters. The historical weights are applied to the charging and discharging parameters to improve the estimated performance of the EECM in estimating the terminal voltage of the battery. Also, the improved EECM is applied to the extended Kalman filter to improve the performance of SOC estimation. A 21,700 cylindrical lithium nickel manganese cobalt oxide‐type battery was used for verification, and the parameters required for modeling were extracted through experiments that were conducted to determine electrical characteristics. This paper compared two models not considering the history of charging or discharging characteristics with a model considering history to verify the accuracy of voltage and SOC estimation results. The result shows that the voltage and SOC estimation errors of the proposed combined method are the lowest in the three cases.
Thermoelectric properties of Sr‐doped LSCO for energy harvesting applications below room temperature
Authors: Saif Mohd , Tripathi Dinesh 
Published: July 2022

AbstractThermoelectric properties of polycrystalline La2CuO4 with (x = 0.0, 0.02, 0.05, 0.10, 0.15, 0.20) have been examined. For this, the samples have been synthesised via solid‐state reaction (SSR) technique. For microstructural analysis of investigated pellets, X‐ray diffraction and scanning electron microscopy have been performed. The electrical resistivity and Seebeck coefficient of the investigated samples have been measured with the help of an in‐house fabricated setup, capable to measure the resistivity and thermopower between 100 and 400 K with ±3% experimental uncertainty. The electrical resistivity reveals a semiconducting nature for pristine pellets. However, a transition to metallic nature of electrical resistivity is observed for Sr doping concentration ≥ 0.02. The Seebeck coefficient (S) of investigated pellets is found to be positive, which indicates holes type conduction. Apart from that, a gradual decrease in the Seebeck coefficient has also been noticed as Sr dose increases. Again, the sample with Sr concentration x = 0.02 is found to be the best with a maximum power factor value (6.947 μW/cm K2) at 165 K, which is a positive sign for future applicability of LSCO‐based oxide materials for energy harvesting applications below room temperature.
Study on the impact of temperature, salts, sugars and pH on dilute solution properties of Lepidium perfoliatum seed gum
Authors: Yousefi Alireza , Elmarhoum Said , Khodabakhshaghdam Shahla , Ako Komla , Hosseinzadeh Ghader 
Published: July 2022

AbstractThe functional properties of food gums are remarkably affected by the quality of solvent/cosolutes and temperature in a food system. In this work, for the first time, the chemical characterizations and dilute solution properties of Lepidium perfoliatum seed gum (LPSG), as an emerging carbohydrate polymer, were investigated. It was found that xylose (14.27%), galacturonic acid (10.70%), arabinose (9.07%) and galactose (8.80%) were the main monosaccharaide components in the LPSG samples. The uronic acid content of LPSG samples was obtained to be 14.83%. The average molecular weight and polydispersity index of LPSG were to be 2.34 × 105 g/mol and 3.3, respectively. As the temperature was increased and the pH was decreased and the concentration of cosolutes (Na+, Ca2+, sucrose and lactose) presented in the LPSG solutions was enhanced, the intrinsic viscosity [η] and coil dimension (Rcoil, Vcoil, υs) of LPSG molecular chains decreased. Activation energy and chain flexibility of LPSG were estimated to be 0.46 × 107 J/kg.mol and 553.08 K, respectively. The relative stiffness parameter (B) of LPSG in the presence of Ca2+ (0.079) was more than that of Na+ (0.032). Incorporation of LPSG into deionized water (0.2%, w/v) diminished the surface activity from 76.75 mN/m to 75.70 mN/m. Zeta potential (ζ) values (−46.85 mV–−19.63 mV) demonstrated that dilute solutions of LPSG had strong anionic nature in the pH range of 3–11. The molecular conformation of LPSG was random coil in all the selected solution conditions. It can be concluded that temperature and presence of cosolutes can significantly influence on the LPSG properties in the dilute systems.
Physiological symmetry of transcranial magnetic stimulation‐evoked EEG spectral features
Authors: D'Ambrosio Sasha , Jiménez‐Jiménez Diego , Silvennoinen Katri , Zagaglia Sara , Perulli Marco , Poole Josephine , Comolatti Renzo , Fecchio Matteo , Sisodiya Sanjay M. , Balestrini Simona 
Published: July 2022

AbstractTranscranial magnetic stimulation (TMS)‐evoked EEG potentials (TEPs) have been used to study the excitability of different cortical areas (CAs) in humans. Characterising the interhemispheric symmetry of TMS‐EEG may provide further understanding of structure–function association in physiological and pathological conditions. We hypothesise that, in keeping with the underlying cytoarchitectonics, TEPs in contralateral homologous CAs share similar, symmetric spectral features, whilst ipsilateral TEPs from different CAs diverge in their waveshape and frequency content. We performed single‐pulse (<1 Hz) navigated monophasic TMS, combined with high‐density EEG with active electrodes, in 10 healthy participants. We targeted two bilateral CAs: premotor and motor. We compared frequency power bands, computed Pearson correlation coefficient (R) and Correlated Component Analysis (CorrCA) to detect divergences, as well as common components across TEPs. The main frequency of TEPs was faster in premotor than in motor CAs (p < .05) across all participants. Frequencies were not different between contralateral homologous CAs, whilst, despite closer proximity, there was a significant difference between ipsilateral premotor and motor CAs (p > .5), with frequency decreasing from anterior to posterior CAs. Correlation was high between contralateral homologous CAs and low between ipsilateral CAs. When applying CorrCA, specific components were shared by contralateral homologous TEPs. We show physiological symmetry of TEP spectral features between contralateral homologous CAs, whilst ipsilateral premotor and motor TEPs differ despite lower geometrical distance. Our findings support the role of TEPs as biomarker of local cortical properties and provide a first reference dataset for TMS‐EEG studies in asymmetric brain disorders.
Hepatocyte β‐catenin loss is compensated by Insulin‐mTORC1 activation to promote liver regeneration
Authors: Hu Shikai , Cao Catherine , Poddar Minakshi , Delgado Evan , Singh Sucha , Singh‐Varma Anya , Stolz Donna Beer , Bell Aaron , Monga Satdarshan P. 
Published: July 2022

Background and Aims:
Liver regeneration (LR) following partial hepatectomy (PH) occurs via activation of various signaling pathways. Disruption of a single pathway can be compensated by activation of another pathway to continue LR. The Wnt–β‐catenin pathway is activated early during LR and conditional hepatocyte loss of β‐catenin delays LR. Here, we study mechanism of LR in the absence of hepatocyte‐β‐catenin.

Approach and Results:
Eight‐week‐old hepatocyte‐specific Ctnnb1 knockout mice (β‐cateninΔHC) were subjected to PH. These animals exhibited decreased hepatocyte proliferation at 40–120 h and decreased cumulative 14‐day BrdU labeling of <40%, but all mice survived, suggesting compensation. Insulin‐mediated mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) activation was uniquely identified in the β‐cateninΔHC mice at 72–96 h after PH. Deletion of hepatocyte regulatory‐associated protein of mTOR (Raptor), a critical mTORC1 partner, in the β‐cateninΔHC mice led to progressive hepatic injury and mortality by 30 dys. PH on early stage nonmorbid RaptorΔHC‐β‐cateninΔHC mice led to lethality by 12 h. RaptorΔHC mice showed progressive hepatic injury and spontaneous LR with β‐catenin activation but died by 40 days. PH on early stage nonmorbid RaptorΔHC mice was lethal by 48 h. Temporal inhibition of insulin receptor and mTORC1 in β‐cateninΔHC or controls after PH was achieved by administration of linsitinib at 48 h or rapamycin at 60 h post‐PH and completely prevented LR leading to lethality by 12–14 days.

Conclusions:
Insulin‐mTORC1 activation compensates for β‐catenin loss to enable LR after PH. mTORC1 signaling in hepatocytes itself is critical to both homeostasis and LR and is only partially compensated by β‐catenin activation. Dual inhibition of β‐catenin and mTOR may have notable untoward hepatotoxic side effects.
Familial Pseudohypoparathyroidism Type IB Associated with an SVA Retrotransposon Insertion in the GNAS Locus
Authors: Kawashima Sayaka , Yuno Akiko , Sano Shinichiro , Nakamura Akie , Ishiwata Keisuke , Kawasaki Tomoyuki , Hosomichi Kazuyoshi , Nakabayashi Kazuhiko , Akutsu Hidenori , Saitsu Hirotomo , Fukami Maki , Usui Takeshi , Ogata Tsutomu , Kagami Masayo 
Published: July 2022

ABSTRACT
Loss of methylation (LOM) at GNAS-A/B:TSS-differentially methylated regions (DMRs) in the GNAS locus is observed in pseudohypoparathyroidism type 1B (PHP1B). Many PHP1B cases are sporadic, but autosomal dominant-PHP1B has a deletion involving NESP55 expressed from the maternal allele or STX16 located upstream of the GNAS locus on the maternal allele. We report the possible first familial PHP1B cases with retrotransposon insertion in the GNAS locus on the maternal allele. To our knowledge, they are the possible first cases with imprinting disorders caused by retrotransposon insertion. The two sibling cases experienced tetany and/or cramps from school age and had hypocalcemia and an increased serum intact parathyroid hormone (PTH) level together with overweight, round face, and normal intellectual levels. Methylation analysis for DMRs in the GNAS locus showed only LOM of the GNAS-A/B:TSS-DMR. Copy number abnormalities at STX16 and the GNAS locus were not detected by array comparative genomic hybridization. Whole-genome sequencing and Sanger sequencing revealed an approximately 1000-bp SVA retrotransposon insertion upstream of the first exon of A/B on the GNAS locus in these siblings. Whole-genome methylome analysis by Enzymatic Methyl-Seq in the siblings showed normal methylation status in the region surrounding the insertion site and mild LOM of the GNAS-A/B:TSS-DMR. We conducted transcriptome analysis using mRNA from skin fibroblasts and induced pluripotent stem cells (iPSCs) derived from the siblings and detected no aberrant NESP55 transcripts. Quantitative reverse-transcriptase PCR (qRT-PCR) analysis in skin fibroblasts showed increased A/B expression in the patients and no NESP55 expression, even in a control. qRT-PCR analysis in iPSCs showed decreased NESP55 expression with normal methylation status of the GNAS-NESP:TSS-DMR in the patients. The retrotransposon insertion in the siblings likely caused decreased NESP55 expression that could lead to increased A/B expression via LOM of the GNAS-A/B:TSS-DMR, subsequent reduced Gsα expression, and finally, PHP1B development. © 2022 American Society for Bone and Mineral Research (ASBMR).
The pyrethroid insecticide permethrin confers hepatotoxicity through DNA damage and mitochondria‐associated apoptosis induction in rat: Palliative benefits of Fumaria officinalis
Authors: Aoiadni Nissaf , Chiab Nour , Jdidi Hajer , Gargouri Bouzid Radhia , El Feki Abdelfattah , Fetoui Hamadi , Ghorbel Koubaa Fatma  
Published: July 2022

AbstractPermethrin (PER) is a pyrethroid pesticide that is extensively used as an insecticide in world because of its high activity and its low mammalian toxicity. The current study was conducted to investigate the protective action of Fumaria officinalis against PER‐induced liver injury in male rats. However, HPLC‐DAD showed the richness of 6 components in F. officinalis (F) including quercetin, ferulic acid, and naringenin which were the most abundant. Total polyphenols, total flavonoids, and condensed tannins were studied by phytochemical screening. In vitro, antioxidant properties showed that F. officinalis exhibited the highest DPPH radical, FRAP, and H2O2 tests and total antioxidant capacity. Wistar rats were divided into four groups: negative control group (C), positive control group (F) (200 mg F. officinalis/kg BW), PER group (34.05 mg permethrin/kg BW), and PER + F group (34.05 mg permethrin/kg BW and 200 mg F. officinalis/kg BW). Oral administration of PER led to promote a decrease of body weight and Ca2+‐ATPases and Mg2+‐ATPases activities and an increase of plasma C‐reactive protein level, transaminases, and hepatic ϒ‐GT activities as well as hepatic and mitochondrial oxidative stress. An increase in plasma lactate‐to pyruvate ratio and a reduction in complexes enzymes I, III, and IV activities were also observed. In addition, histoarchitecture of liver in PER‐treated rats showed apoptosis and necrosis as confirmed by DNA fragmentation. F. officinalis significantly exerted hepatoprotective effect by modulating hepatic alteration and mitochondrial dysfunction as well as genotoxicity. This effect could be attributed to phenolics compounds such as polyphenols, condensed tannins, and flavonoids.
Automated input structure generation for single‐ended reaction path optimizations
Authors: Geiger Julian , Settels Volker , Deglmann Peter , Schäfer Ansgar , Bergeler Maike 
Published: July 2022

AbstractThe exploration of a reaction network requires highly automated workflows to avoid error‐prone and time‐consuming manual steps. In this respect, a major bottleneck is the search for transition‐state (TS) structures, which frequently fails and, therefore, makes (manual) revision necessary. In this work, we present a technique for obtaining suitable input structures for automated TS searches based on single‐ended reaction path optimization algorithms, which makes subsequent TS searches via this method significantly more robust. First, possible input structures are generated based on the spatial alignment of the reactants. The appropriate orientation of reacting groups is achieved via stepwise rotations along selected torsional degrees of freedom. Second, a ranking of the obtained structures is performed according to selected geometric criteria. The main goals are to properly align the reactive atoms, to avoid hindrance within the reaction channel and to resolve steric clashes between the reactants. The developed procedure has been carefully tested on a variety of examples and provides suitable input structures for TS searches within seconds. The method is in daily use in an industrial setting.
Targeting MYO1B impairs tumorigenesis via inhibiting the SNAI2/cyclin D1 signaling in esophageal squamous cell carcinoma
Authors: Li Shau‐Hsuan , Qian Limei , Chen Yen‐Hao , Lu Hung‐I , Tsai Hsin‐Ting , Lyu Haiwen , Yang Runxiang , Chen Chang‐Han 
Published: July 2022

AbstractMyosin‐related proteins play an important role in cancer progression. However, the clinical significance, biological functions, and mechanisms of myosin 1B (MYO1B), in esophageal squamous cell carcinoma (ESCC) remain unclear. The clinical relevance of MYO1B, SNAI2, and cyclin D1 in ESCC was determined by immunohistochemistry, Oncomine, and GEPIA databases. The oncogenic roles of MYO1B were determined by CCK8, colony formation assays, wound healing, and Transwell assay. MYO1B, SNAI2, and cyclin D1 at mRNA and protein levels in ESCC cells were detected by qPCR and Western blot analysis. In our study, we found that MYO1B expression was increased in ESCC tissue samples and correlated with tumor stage, TNM stage, and poor outcomes. Functional assays indicated that depletion of MYO1B impaired oncogenesis, and enhanced chemosensitivity in ESCC. Bioinformatic analysis and mechanistic studies illustrated that SNAI2 was a key downstream effector of MYO1B. Suppression of MYO1B downregulated expression of SNAI2, thereby inhibiting the SNAI2/cyclin D1 pathway. Furthermore, a selective inhibitor of cyclin D1 activation reversed siMYO1B cells overexpressing SNAI2‐elicited aggressive phenotypes of ESCC cells. MYO1B positively correlated with SNAI2 and cyclin D1 in ESCC samples, and higher SNAI2 expression was also associated with poor prognosis in ESCC patients. Our finding demonstrated that MYO1B activates the SNAI2/cyclin D1 pathway to drive tumorigenesis and cisplatin cytotoxicity in ESCC, indicating that MYO1B is a potential therapeutic target for patients with ESCC.
Evidence of an Annexin A4 mediated plasma membrane repair response to biomechanical strain associated with glaucoma pathogenesis
Authors: Vicic Nevena , Guo Xiaoxin , Chan Darren , Flanagan John G , Sigal Ian A. , Sivak Jeremy M. 
Published: July 2022

AbstractGlaucoma is a common neurodegenerative blinding disease that is closely associated with chronic biomechanical strain at the optic nerve head (ONH). Yet, the cellular injury and mechanosensing mechanisms underlying the resulting damage have remained critically unclear. We previously identified Annexin A4 (ANXA4) from a proteomic analyses of human ONH astrocytes undergoing pathological biomechanical strain that mimics glaucomatous conditions. Annexins are a family of calcium‐dependent phospholipid binding proteins with key functions in plasma membrane repair (PMR); an active mechanism to limit and mend cellular injury that involves membrane and cytoskeletal reorganizations. However, a role for direct membrane damage and PMR has not been well studied in the context of biomechanical strain, such as that associated with glaucoma. Here we report that this moderate strain surprisingly damages cell membranes to increase permeability in a calcium‐dependent manner, and induces rapid aggregation of ANXA4 at injury sites. ANXA4 loss‐of‐function increases permeability, while exogenous ANXA4 reduces it. Furthermore, ANXA4 aggregation is associated with F‐actin dynamics in vitro, and remarkably this interaction and aggregation signature is also observed in the glaucomatous ONH in patient samples. Together these studies link moderate biomechanical strain with direct membrane damage and actin dynamics, and identify an active PMR role for ANXA4 in new model of cell injury associated with glaucoma pathogenesis.
Molecular networks underlying cannabinoid signaling in skeletal muscle plasticity
Authors: Dalle Sebastiaan , Schouten Moniek , Meeus Gitte , Slagmolen Lotte , Koppo Katrien 
Published: July 2022

AbstractThe cannabinoid system is ubiquitously present and is classically considered to engage in neural and immunity processes. Yet, the role of the cannabinoid system in the whole body and tissue metabolism via central and peripheral mechanisms is increasingly recognized. The present review provides insights in (i) how cannabinoid signaling is regulated via receptor‐independent and ‐dependent mechanisms and (ii) how these signaling cascades (might) affect skeletal muscle plasticity and physiology. Receptor‐independent mechanisms include endocannabinoid metabolism to eicosanoids and the regulation of ion channels. Alternatively, endocannabinoids can act as ligands for different classic (cannabinoid receptor 1 [CB1], CB2) and/or alternative (e.g., TRPV1, GPR55) cannabinoid receptors with a unique affinity, specificity, and intracellular signaling cascade (often tissue‐specific). Antagonism of CB1 might hold clues to improve oxidative (mitochondrial) metabolism, insulin sensitivity, satellite cell growth, and muscle anabolism, whereas CB2 agonism might be a promising way to stimulate muscle metabolism and muscle cell growth. Besides, CB2 ameliorates muscle regeneration via macrophage polarization toward an anti‐inflammatory phenotype, induction of MyoD and myogenin expression and antifibrotic mechanisms. Also TRPV1 and GPR55 contribute to the regulation of muscle growth and metabolism. Future studies should reveal how the cannabinoid system can be targeted to improve muscle quantity and/or quality in conditions such as ageing, disease, disuse, and metabolic dysregulation, taking into account challenges that are inherent to modulation of the cannabinoid system, such as central and peripheral side effects.
Impact of preoperative muscle mass and quality on surgical outcomes in patients undergoing major hepatectomy for perihilar cholangiocarcinoma
Authors: Asai Yuichi , Yamaguchi Junpei , Mizuno Takashi , Onoe Shunsuke , Watanabe Nobuyuki , Igami Tsuyoshi , Uehara Kay , Yokoyama Yukihiro , Ebata Tomoki 
Published: July 2022

AbstractPurposesThis study sought to define the impact of skeletal muscle mass and quality on postoperative outcomes in patients with perihilar cholangiocarcinoma.MethodsPatients who underwent major hepatectomy for perihilar cholangiocarcinoma were included. The normalized total psoas area (TPA) (psoas muscle index [PMI]) and average Hounsfield units of the TPA (psoas muscle density [PMD]) were measured using preoperative computed tomography images. The cohort was dichotomized using the following indices: sex‐specific lowest tertile (low PMI and low PMD) and other (normal PMI and normal PMD). Intraoperative and postoperative outcomes were compared, focusing on PMI and PMD.ResultsA total of 456 patients were analyzed. The intraoperative blood loss (IBL) was 21.3 ml/kg in the low PMI group and 17.2 ml/kg in the normal PMI group (P = .008). Patients in the low PMI or PMD group experienced postoperative infectious complications more frequently than those in the other groups. The median survival time was 37.8 months in the low PMI group and 54.2 months in the normal PMI group (P = .027).ConclusionsPMI and PMD were closely associated with IBL and postoperative infectious complications. Additionally, PMI impacted long‐term survival. These results suggest an importance of improving muscle mass and quality before surgery.
Ultrasonic‐Assisted condensation of aromatic and aliphatic aldehydes with 3‐(Thiophen‐2‐yl)‐5‐Pyrazolone: Synthesis, characterization and Stereoselective application
Authors: Rizk Hala F. , Noser Ahmed A. , Ibrahim Seham A. , Fares Amira K. 
Published: July 2022

AbstractA green and efficient protocol for a one‐pot condensation of 1‐(2,4‐dinitrophenyl)‐3‐(thiophen‐2‐yl)‐1H‐pyrazol‐5(4H)‐one (1) with aromatic and aliphatic aldehydes in different ratios to synthesize novel heterocyclic compounds containing pyrazolone and pyranopyrazole moieties. The reactions were conducted using the ultrasonic and traditional methods. The sonication ensured more efficiency in the rate and yield of the reactions. The structures of the synthesized compounds were elucidated by different spectroscopic methods. Additionally, an enantioselective synthesis of (R)‐ and (S)‐α‐alkylated acid was achieved using 1‐(2,4‐dinitrophenyl)‐4‐(hydroxymethyl)‐3‐(thiophen‐2‐yl)‐1H‐pyrazol‐5(4H)‐one (6) as chiral auxiliary, (S)‐4‐Methyl‐5‐nonanol the aggregation pheromone of red palm weevil was efficiently prepared and used for trapping the red palm weevil.