bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple effects of immune and cuproptosis related LNCRNAs on lung adenocarcinoma and the related expression was verified by RT-PCR
Authors: Sun Kang , Zhang Zhiqiang , Wang Dongqin , Huang Yinlong , Zhang Jing , Lian Chaoqun 
Published: July 2022

Abstract
Background: Lung adenocarcinoma is still a high mortality tumor disease. Immune cells play an important role in the further development of tumors, including NK and CD8 cells. Recent studies have found that cuproptosis is a new way of cell death induced by copper ions.Objective: Therefore, the purpose of this study was to screen the prognostic LNCRNAs associated with immunity and cuproptosis.Method: Based on TCGA and geo databases, LNCRNAs (IMMCusLNCRNAs) related to immunity and cuproptosis were obtained according to the grouping of NK and CD8 cells and genes related to copper filament death. Based on a variety of statistical and bioinformatics methods, an immcusLNCRNAs related risk model was established to evaluate the correlation between the model and prognosis. Using various immune cell scoring models in TIMER database, the distribution differences of immune cells in different risk groups were analyzed. Then, to explore the distribution of glycolysis and chemosensitivity in different risk groups. Finally, the expression levels of risk genes between lung epithelial cells and other lung adenocarcinoma cells were compared.Results: risk genes were closely related to prognosis. NK and CD8 cells were generally distributed in the low-risk group, while the glycolytic pathway was the opposite. Under different risk groups, the sensitivity of chemotherapy drugs is also very different. Finally, the expression of risk genes in normal lung epithelial cells is usually higher than that in other lung adenocarcinoma cells.Conclusion: the risk model related to immunity and cuproptosis and LNCRNAs provide an important idea for personalized speech therapy.
Distinct types of multicellular aggregates in Pseudomonas aeruginosa liquid cultures
Authors: melaugh Gavin , Martinez Vincent , Baker Perrin , Hill Preston , Howell P. Lynne , Wozniak Daniel , Allen Rosalind 
Published: July 2022

Abstract
Pseudomonas aeruginosa forms suspended multicellular aggregates when cultured in liquid media. Such aggregates may be important in disease, and/or as a pathway to biofilm formation. The polysaccharide Psl and extracellular DNA (eDNA) have both been implicated in aggregation, but previous results depend strongly on the experimental conditions. Here we develop a quantitative microscopy-based method for assessing changes in the size distribution of suspended aggregates over time in growing cultures. For exponentially growing cultures of P. aeruginosa PAO1, we find that aggregation is mediated by cell-associated Psl, rather than by either eDNA or secreted Psl. These aggregates arise de novo within the culture via a growth process that involves both collisions and clonal growth. They are "non-cheatable" since Psl non-producing cells do not aggregate with producers. In contrast, we find that stationary phase (overnight) cultures contain a different type of multicelullar aggregate, in which both eDNA and Psl mediate cohesion. Our findings suggest that the physical and biological properties of multicellular aggregates may be very different in early-stage vs late-stage bacterial cultures.
Optical binding and manipulation of microparticles in fluidic flow
Authors: Hu Yanjun , Li Ying 
Published: July 2022

Abstract
Controllable organization and manipulation of particles has recently gain significant attention in biological and medical studies. In this work, we demonstrated optical binding and manipulation of 3 µm SiO2 particles in fluidic flow using a tapered optical fiber (TOF). Particles can be organized into one-dimension chain with different numbers by the cooperation of optical scattering force, binding force and viscous drag force. The particle chain can be shifted back and forth along the axis of the TOF by adjusting optical power or flow velocity.
Adverse Drug Events and Contributing Factors Among Pediatric Cancer Patients at Jimma University Medical Center, Southwest Ethiopia
Authors: Tola Wayessa Olika , Melaku Tsegaye , Fufa Diriba , Sheleme Tadesse 
Published: July 2022

Abstract
Background: The characteristics and incidence of adverse drug events (ADEs) among pediatric cancer patients in developing countries were not well characterized. ADEs & medication errors associated with cancer chemotherapy in children need to be analyzed on their incidence and severity. The purpose of this study was hence, to assess the incidence of adverse drug events and contributing factors among pediatric cancer patients at Jimma university medical center, Jimma, Ethiopia Method: A prospective observational method was used to study adverse drug events in pediatrics admitted to pediatric oncology unit of Jimma University medical center between October and December 2020. The ADEs were identified using multifaceted approaches involving daily chart review, interview of Parent/care giver(and or children themselves), Attendance at ward rounds and voluntary staff reports. Both univariate and multivariate logistic regression was used to assess the predictors of the identified ADEs. Those factors that showed association at p value less than 0.25 in the univariate analysis were added to backward multivariate logistic regression model and the significant association was checked at p value < 0.05.Result: a total of 73 (46 male and 27 female) patients were included in the study and a total of 466 ADEs. Four hundred sixty six adverse drug events were identified with an incidence of 638.36 ADEs per 100 patients, 38.35 ADEs per 100 patient days and 2.34 ADEs per chemotherapy cycle. The most common ADEs were hematologic toxicities (anemia 55(11.8%), neutropenia 52(11.16%) & thrombocytopenia 31(6.65%)), and gastrointestinal effects (nausea 46(9.87%), vomiting 46(9.87%), anorexia 41(8.8%). Out of 466 ADEs, 150 were classified as Grade 1, 199 as Grade 2, 64 as Grade 3, 48 as grade 4 and 5 as Grade 5. Conclusion: Adverse drug events were common in the pediatric oncology ward of JUMC. In particular, children with multiple chemotherapy drugs and those with comorbid condition were at greater risk for adverse drug events.
Inactivation of VEGFB Blocks Tumor Growth and Metastasis by Skewing Macrophages to M1 Phenotype
Authors: Chen Yang , Li Xin , Wen Huaizhen , Lu Mingxu , Adlat Salah , Li Dan , Zhao Mingyue , Lu Xiaodan , Zhang Mingjiao , Zhang Yanjiao , Sah Rajiv Kumar , Chen Quangang , Han Xufeng , Wang Yonghui , Chen Renjin , Choudhury Mahua , Feng Xuechao , Zheng Yaowu 
Published: July 2022

Abstract
VEGFB has high sequence homology and shares receptor VEGFR1 with VEGFA and PlGF. However, its function is poorly understood. Using VEGFA repression (VegfaDown), VEGFB knockout (Vegfb−/−) and VEGFB over expression (aP2-Vegfb167, aP2-Vegfb186) mouse models, we identified VEGFB167 as a strong promoter of tumor growth. Universal Vegfb inactivation significantly retards tumor growth and metastasis of B16 and U14 cancer cells. VEGFB deletion led to an inactivation of VEGFR1 pathway, blocking of tumor-associated macrophage transformation towards M1 phenotype and creation of a microenvironment that represses tumor growth and metastasis. Genetic VEGFA repression showed reduced inhibitory effect on U14 and no effect on B16 tumor growth in comparison to VEGFB. The inhibitory effect by inactivating VEGFB on tumor growth and tumor cell metastasis is significantly better than VEGFA. The study demonstrates VEGFB is an oncogene and drug target for VEGFB-sensitive cancers.
Function and autophagy of monocyte-derived dendritic cells is affected by hepatitis B virus infection
Authors: Xu Hua , Kang Juan , Zhong Shan , Chen Min , Hu Peng , Ren Hong , Zhou Zhi , Lei Yu 
Published: July 2022

Abstract
Background: The role of DCs and the autophagy state of DCs in the immune response of hepatitis B virus (HBV) infection was still controversial. In this study, we carefully examined the phenotype, function and autophagy pathway of DCs in HBV infection. Methods: Monocytes-derived DCs from healthy blood donors and patients with chronic HBV infection were stimulated by LPS, supernatant of HepG2.2.15 cells or supernatant of HepG2 cells respectively. Phenotype of DCs was examined by flow cytometry and cytokines secretion was detected by ELISA. Autophagy related proteins were detected by western blot and immunofluorescence analysis. Results: Our results showed that the expression of both MHC II molecules and co-stimulated molecules including CD80, CD86 in the monocytes-derived DCs from patients with chronic HBV infection was significantly higher than that from healthy donors when cultured with supernatant of HepG2.2.15 cells. The amount of cytokines, including TNF-α, IL-10 and IL-12, secreted by monocytes-derived DCs from patients with chronic HBV infection was also significantly higher than that from healthy donors when stimulate by HBV. Interestingly, the expression level of autophagy-related proteins including Atg-5 and LC3 in DCs from patients with chronic HBV infection was significantly increased when compared with that from healthy donors when re-exposed to HBV. Conclusions: Our results indicated that DCs from patients with chronic HBV infection could intensively present antigen and express co-stimulate molecules. The increased activation of DCs might be related to the enhanced autophagy of DCs in HBV infection.
Palliative primary tumor resection may not offer survival benefits for patients with unresectable metastatic colorectal neuroendocrine neoplasms, one multicenter retrospective cohort study
Authors: Wang Zhijie , Ren Hongchang , Li Li , Liu Qian 
Published: July 2022

Abstract
Background: The efficacy of palliative primary tumor resection (PTR) in improving prognosis for patients with unresectable metastatic colorectal NENs has not been fully explored.Methods: We performed one retrospective cohort study and recruited 68 patients with metastatic colorectal NENs from two Chinese medical centers. The clinicopathological manifestation data were carefully collected, and the survival outcomes were compared between the two groups.Results: A total of 32 patients received PTR, and the other 36 patients did not. The median progression-free survival (PFS) and overall survival (OS) times were 4 and 20 months in the cohort, respectively. For patients who received no PTR, the median OS was 31 months, and the 1-year OS rate and 3-year OS rate were 61% and 42.5%, respectively. For patients who received PTR, the median OS was 24 months, and the 1-year OS rate and 3-year OS rate were 70.5% and 27.9%, respectively. However, the Kaplan–Meier survival curves and log-rank test demonstrated no significant survival difference between the two groups (P=0.585). Palliative PTR was also not confirmed as a prognostic factor in subsequent univariable and multivariable analyses.Conclusions: Palliative PTR may not offer survival benefits for patients with unresectable metastatic colorectal NENs.
7,8-Dihydroxyflavone induces mitochondrial apoptosis and down-regulates the expression of ganglioside GD3 in malignant melanoma cells
Authors: Ju Won Seok , Seo Sang Young , Mun Seong-Eun , Yu Jin Ok , Ryu Jae-Sung , Kim Ji-Su , Choo Young-Kug 
Published: July 2022

Abstract
Malignant melanoma is one of the most progressive skin cancers, with a poor prognosis, various side effects, and high resistance to conventional treatment. Recently, 7,8-dihydroxyflavone (7,8-DHF), a polyphenolic flavonoid derived from certain plants, has been studied for its anti-carcinogenic, anti-inflammatory, antioxidant, and pharmacological effects in several types of cancer. Ganglioside, a modulator of diverse cell signals on the microdomain of the surface of the cell membrane, is closely involved in various cancers. However, the correlation between ganglioside expression and the anti-cancer effects of 7,8-DHF in malignant melanoma remains unclear. In this study, 7,8-DHF showed potential as an anti-cancer agent through specific anti-proliferation, anti-oxidant, anti-migration, pro-apoptotic, and G2/M phase cell cycle arrest effects on SK-MEL-2 and G-361 melanoma cells. In contrast, 7,8-DHF did not induce cytotoxicity in non-tumoral epidermal HaCaT cells. Additionally, we confirmed for the first time that 7,8-DHF significantly reduces the expression levels of ganglioside GD3, which is closely involved in carcinogenesis, in both melanoma cancer cell lines using high-performance thin-layer chromatography analysis. Taken together, our findings suggest that 7,8-DHF might be a potent anticancer drug candidate for the treatment of malignant melanoma.
Lower Bounds for QCDCL via Formula Gauge
Authors: Böhm Benjamin , Beyersdorff Olaf 
Published: July 2022

Abstract
QCDCL is one of the main algorithmic paradigms for solving quantified Boolean formulas (QBF).
We design a new technique to show lower bounds for the running time in QCDCL algorithms. For this we model QCDCL by concisely defined proof systems and identify a new width measure for formulas, which we call gauge. We show that for a large class of QBFs, large (e.g. linear) gauge implies exponential lower bounds for QCDCL proof size.
We illustrate our technique by computing the gauge for a number of sample QBFs, thereby providing new exponential lower bounds for QCDCL. Our technique is the first bespoke lower bound technique for QCDCL.
Biochar and organic fertilizer amendments improved soil microbial biomass, soil quality, and wheat yield of saline-alkali soils in a three-year field trial
Authors: Wang shibin , gao peiling , zhang qingwen , Shi yulong , guo xianglin , lv qingxin , wu wei , zhang xue , Li mengzhao , meng qingmei 
Published: July 2022

Abstract
The objective of this study was to examine the effects of biochar compared to organic fertilizer on soil quality and microbial biomass as well as wheat yield in the saline-alkaline lands. A three-year field trial was conducted on moderately saline-alkaline land in the Yellow River Delta region (YRD) with six treatments: biochar (B1: 5 t, B2: 10 t, B3: 20 t ha− 1 year− 1) and organic fertilizer (OF1: 5 t, OF2: 7.5 t ha− 1 year− 1) as well as control (CK). The results showed that both biochar and organic fertilizer increased total organic carbon (TOC), total nitrogen (TN), NH4+-N, and NO3−-N, and reduced pH, thereby increasing soil microbial biomass carbon (MBC) and nitrogen (MBN), MBC/TOC ratio, and MBN/TN ratio, but organic fertilizer increased soil nutrients and microbial biomass better than biochar. Correlation analysis revealed that soil water content (SWC), soil salt content (SSC), and Na+ were the most important factors influencing wheat yield. When compared to CK, the SSC and Na+ decreased by 5.55–7.52% and 3.86–9.39%, respectively, and SWC increased by 5.14–5.62% in the biochar treatment, while they increased by 1.07–10.19%, 1.08–7.58%, and 2.96–3.84% in the organic fertilizer treatment, respectively. Accordingly, wheat yield in biochar treatment was 0.90–14.20% higher than organic fertilizer treatment (4.49–4.80 t ha− 1). Collectively, B2 had the lowest SSC and Na+ and the highest yield, as well as efficiently increasing soil nutrients and microbial biomass, suggesting that it may be a better agricultural practice for improving soil quality and increasing wheat yield in the YRD.
Accurate and fast identification of minimally prepared bacteria phenotypes using Raman spectroscopy assisted by machine learning
Authors: Lassen Mikael , Thomsen Benjamin Lundquist , Christensen Jesper , Rodenko Olga , Usenov Iskander , Grønnemose Rasmus Birkholm , Andersen Thomas Emil 
Published: July 2022

Abstract
The worldwide increase of antimicrobial resistance (AMR) is a serious threat to human health. To avert the spread of AMR, fast reliable diagnostics tools that facilitate optimal antibiotic stewardship are an unmet need. In this regard, Raman spectroscopy promises rapid label- and culture-free identification and antimicrobial susceptibility testing (AST) in a single step. However, even though many Raman-based bacteria-identification and AST studies have demonstrated impressive results, some shortcomings must be addressed. To bridge the gap between proof-of-concept studies and clinical application, we have developed machine learning techniques in combination with a novel data-augmentation algorithm, for fast identification of minimally prepared bacteria phenotypes and the distinctions of methicillin-resistant (MR) from methicillin-susceptible (MS) bacteria. For this we have implemented a spectral transformer model for hyper-spectral Raman images of bacteria. We show that our model outperforms the standard convolutional neural network models on a multitude of classification problems, both in terms of accuracy and in terms of training time. We attain more than 96$\%$ classification accuracy on a dataset consisting of 15 different classes and 95.6$\%$ classification accuracy for six MR-MS bacteria species. More importantly, our results are obtained using only fast and easy-to-produce training and test data.
Intensive stretch-activated CRT-PMCA1 feedback loop promoted Ca2+ overloading and apoptosis of myoblasts
Authors: Ren Dapeng , liu Ran , Yan Xiao , Zhang Qiang , Zeng Xuemin , Yuan Xiao 
Published: July 2022

Abstract
Background
Mechanical stretch exerted pro-apoptotic effect on myoblasts, the mechanism of which is currently unknown. Intracellular Ca2+ accumulation has been implicated in stretch-induced apoptosis. calreticulin (CRT) and plasma membrane Ca2+ transporting ATPase 1 (PMCA1) are two critical components of Ca2+ signaling system participating in intracellular Ca2+ homeostasis. In this study, we explored the contribution of CRT and PMCA1 in mediating stretch-induced Ca2+ accumulation and apoptosis of myoblasts.
Results
Stretching stimuli elevated level of CRT while inhibited activity of PMCA1. Moreover, there were bidirectional regulations between CRT and PMCA1, which formed the positive feedback loop leading to continuous increment of CRT level and repression of PMCA1 activity, in stretched myoblasts. Specifically, increased CRT level inhibited PMCA1 activity via suppressing Calmodulin (CaM), while reduced PMCA1 activity promoted CRT expression through activating p38MAPK pathway. Thus, the CRT-CaM-PMCA1 and PMCA1-p38MAPK-CRT pathways constituted a close cycle comprising CRT, PMCA1, CaM and p38MAPK. Inhibition of both CaM and p38MAPK affected the other three factors in stretched myoblasts. Circulation of the vicious cycle resulted in escalated Ca2+ overloading in myoblasts under continuous stretching stimuli. CRT knock-down, PMCA1 overexpression, and p38MAPK inhibition all attenuated the raised intracellular Ca2+ level and ameliorated myoblast apoptosis in the stretching environment. Conversely, CRT overexpression, PMCA1 knock-down, and CaM inhibition all aggravated stretch-induced Ca2+ overloading and myoblast apoptosis.
Conclusions
A positive feedback loop between CRT and PMCA1 was activated in stretched myoblasts, which contributed to intracellular Ca2+ accumulation and resultant myoblast apoptosis.
Medical Department Classification Based on Patient Describing Symptoms
Authors: Mao Chao , Zhu Quanjing , Chen Rong , Su Weifeng 
Published: July 2022

Abstract
The accurate classification of patients describing symptoms can greatly improve the efficiency of medical department classification, reduce the workload of medical staff, improve the efficiency of patients' registration process, and promote the development of medical informatization. In this study, we proposed a novel neural network based on a hybrid model integrated with an attention mechanism (HMAN), which can automatically match patients to the appropriate department based on the symptoms they described. This system relieves the pressure on the medical staff at the hospital's pre-examination desk and plays a role in diversion for the hospital, more importantly, this model is highly professional and can better avoid the waste of medical resources. Which can effectively improve the work efficiency of the hospital and play a vital role in the long-term development of the hospital. To verify the actual effect of the HMAN, we created a data set of more than 40,000 items, including eight departments, such as ENT, Pediatrics, and other common departments. Self-built dataset experiments reveal that the classification model achieves more than 93.5% accuracy and has a high generalization capacity, which is superior to traditional classification models.
Investigation of damage control strategy for non-traumatic diseases: A single-center observational study
Authors: Nakamura Fumiko , Yui Rintaro , Onoe Atsunori , Kishimoto Masanobu , Sakuramoto Kazuhito , Muroya Takashi , Kajino Kentaro , Ikegawa Hitoshi , Kuwagata Yasuyuki 
Published: July 2022

Abstract
BACKGROUND
Damage control strategy (DCS) has been introduced not only for trauma but also acute abdomen, but its indications and usefulness have not been clarified. We examined clinical characteristics of patients who underwent DCS and compared clinical characteristics and results with and without DCS in patients with septic shock.
METHODS
We targeted a series of endogenous abdominal diseases in Kansai Medical University Hospital from April 2013 to March 2019. Clinical characteristics of 26 patients who underwent DCS were examined. Then, clinical characteristics and results were compared between the DCS group (n = 26) and non-DCS group (n = 31) in 57 patients with septic shock during the same period.
RESULTS
All 26 patients who underwent DCS had septic shock, low mean arterial pressure (MAP) before the start of surgery, and required high-dose norepinephrine administration intraoperatively. Their discharge mortality rate was 12%. Among the patients with septic shock, the DCS group had a higher SOFA score (P = 0.008) and MAP was lower preoperatively, but it did not increase even with intraoperative administration of large amounts of fluid replacement and vasoconstrictor. There was no significant difference in 28-day mortality and discharge mortality between the two groups.
CONCLUSIONS
DCS may be useful in patients with severe septic shock.