bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of Retinogeniculate Synaptic Function in the DBA/2J Mouse Model of Glaucoma
Authors: Smith Jennie C. , Zhang Kevin Yang , Sladek Asia , Thompson Jennifer , Bierlein Elizabeth R. , Bhandari Ashish , Van Hook Matthew J. 
Published: December 2022

AbstractRetinal ganglion cell (RGC) axons comprise the optic nerve and carry information to the dorsolateral geniculate nucleus (dLGN), which is then relayed to the cortex for conscious vision. Glaucoma is a blinding neurodegenerative disease that commonly results from intraocular pressure (IOP)-associated injury leading to RGC axonal pathology, disruption of RGC outputs to the brain, and eventual apoptotic loss of RGC somata. The consequences of elevated IOP and glaucomatous pathology on RGC signaling to the dLGN are largely unknown yet are likely to contribute to vision loss. Here, we used anatomic and physiological approaches to study the structure and function of retinogeniculate (RG) synapses in male and female DBA/2J (D2) mice with inherited glaucoma before and after IOP elevation. D2 mice showed progressive loss of anterograde optic tract transport to the dLGN and vGlut2 labeling of RGC axon terminals while patch-clamp measurements of RG synaptic function showed that synaptic transmission was reduced in 9-month and 12-month D2 mice because of the loss of individual RGC axon inputs. TC neuron dendrites had reduced Sholl complexity at 12 months, suggestive of delayed reorganization following reduced synaptic input. There was no detectable change in RGC density in 11- to 12-month D2 retinas, quantified as the number of ganglion cell layer-residing somata immuno-positive for NeuN and immuno-negative for the amacrine marker choline acetyltransferase (ChAT). Thus, observed synaptic defects appear to precede RGC somatic loss. These findings identify glaucoma-associated and IOP-associated deficits in an important subcortical RGC projection target, shedding light on processes linking IOP to vision loss.
Estrategias de afrontamiento en personal de la salud que atienden pacientes COVID y no COVID en dos ciudades de Colombia
Authors: Ángel Rojas Yohana Alexandra , Suarez Puerto Tania Alejandra , Vásquez Matallana Eduar Andrés , Mena Moncada Erika Paola , Organista Restrepo Natalia Andrea , Rodríguez Cano Carlos Camilo , Rozo Sánchez María Margarita , Moreno Méndez Jaime Humberto 
Published: December 2022

El propósito de este estudio fue comparar las estrategias de afrontamiento en personal de salud de Villavicencio y Bogotá en Colombia que atienden pacientes Covid-19 y No-Covid-19. El diseño fue no experimental, descriptivo comparativo y de corte transversal. La muestra estuvo conformada por 14 hombres (33.3%) y 46 mujeres (76.7%) con edad promedio (M=35.7; DE= 9.72). Se utilizó la Escala de Estrategias de Coping modificada (EEC-M). Se aplicó la t de Student para muestras no relacionadas, la prueba de U de Mann-Whitney y la ANOVA de un factor para la comparación de grupos. Los resultados revelaron que las estrategias de afrontamiento más utilizadas son solución de problemas (M=31.52; DE= 5.24) y búsqueda de apoyo de social (M=20.35; DE= 5.04). Se encontraron diferencias estadísticamente significativas entre las variables: atención a pacientes Covid y sexo en comparación con las estrategias de afrontamiento; sin embargo no hubo diferencias entre grupos por edad, ciudad y profesión. Se resalta que las estrategias que los profesionales de la salud utilizan para afrontar la atención de pacientes Covid y no Covid pueden variar dependiendo de los recursos personales, de la percepción del riesgo y de las causas que lo originen.
Sarcoma fibromixoide de baixo grau em região paratesticular
Authors: Souza Vinícius Gonçalves de , Carvalho Aparecida de Lourdes , Miranda Carla Silva Siqueira 
Published: December 2022

Objetivos: relatar um caso raro de sarcoma fibromixoide de baixo grau (SFMBG) em uma localização incomum de modo a reforçar aspectos histopatológicos e imunoistoquímicos relevantes para o reconhecimento desta entidade e o adequado diagnóstico diferencial de massas paratesticulares. Relato de caso: homem de 20 anos, com massa escrotal à direita, cuja análise histopatológica demonstrou a presença de tecido fibroso com áreas mixoides e predominância de células fusiformes. A imunoistoquímica foi positiva para vimentina, com índice de Ki67 de 2%, e negativa para S100, CD-34, beta-catenina, desmina e miogenina. Conclusões: caso raro de SFMBG na região paratesticular que reforça a importância da histopatologia e da imunoistoquímica no diagnóstico desse tumor. Apesar da característica histológica benigna, o SFMBG apresenta altas taxas de recorrência e metástases, sendo essencial o seguimento do paciente.
Family communication experience among students who are addicted to online games
Authors: Fahrizal Yanuar , Aprilia Friska Dwi 
Published: December 2022

Background: Someone who plays online games continuously will experience feelings of extreme pleasure and want to play the games repeatedly, resulting in online game addiction. The high prevalence of online game addiction causes poor relationships between children and parents since children are too busy playing online games. A considerable amount of time spent playing online games also causes university students to lack opportunities for social interactions. This study aims to explore family communication experiences among university students addicted to online games.Methods: This study adopted a qualitative method with a phenomenological approach, involving eight respondents. The data were collected using in-depth interviews, observations, and documentation. The Indonesian Online Game Addiction Questionnaire was distributed to the participants to determine who fitted into the addiction criteria.Result: This study resulted in five themes as follows: students’ awareness of the importance of family communication; the intensity of communication between students and their parents; the changes in students’ contact with family due to online game addiction; the impact of parenting style on students’ online game addiction; and online game addiction as the cause of conflicts between students and their parents.Conclusion: Parenting style contributes to online game addiction. Online game addiction among students causes changes, especially in the intensity and need for communication with parents despite students’ good awareness of the importance of maintaining contact with their parents.
Kefir Gel Increased The Epithelial Thickness in First-Degree Sunburn
Authors: Kusumawinakhyu Titik , Dharmana Edi , Nasihun Taufiqurrachman , Chodidjah Chodidjah , Pudjonarko Dwi 
Published: December 2022

Background: UVB radiation may cause skin to experience acute symptoms such as erythema and pain. The acute symptoms are categorized as first-degree sunburn. Prevention can be done through kefir gel administration.Purpose: This study aimed to determine differences between effect kefir oral, topical gel, and combination (oral and topical gel) effects and the negative control group on epithelial thickness in first-degree sunburn. Method: An experimental study with post-test only control group design was conducted on BALB/c mice, and the negative control group exposed only with UVB without kefir and oral, topical, and combination (oral and gel) group administration with dose of 0.25 mL/KgBB/day. The epithelial thickness was determined by histopathological preparations of back skin tissue through HE painting. Distribution analysis of data normality was through Shapiro-Wilk test. Homogeneity test of both groups was done through Levene test. One-way Anova test was continued with Post Hoc TukeyHSD test for epithelial thickness.Result: The analysis of Post Hoc TukeyHSD test showed p score <0.05 which meant that there was a significantly higher difference in epithelial thickness of topical group (12.59 ± 2.067) than the negative control (5.30 ± 0.353). Kefir gel dose of 0.25mL/kgBW/day escalates thickness of epithelialization compared to negative control group.Conclusion: There is a significant difference in epithelial thickness between the negative control group and the topical kefir gel treatment groups and the combination of both (oral and topical).
Aqueous Humour Malondialdehyde Level as Oxidative Stress Marker In Types Of Glaucoma
Authors: Maharani Maharani , Dewi Puspita Kusuma , Prihatningtias Riski , Wildan Arief , Nugroho Trilaksana , Limijadi Edward Kurnia Setiawan , Rahmi Fifin L. 
Published: December 2022

Background: Angle-closure glaucoma is the most common type of glaucoma in Asia, leading worse damage due to its oxidative stress. Another type is open angle glaucoma in second line. The oxidative stress level can be known by malondialdehyde level examination. The study about MDA levels in aqueous humour in primary angle-closure and open angel glaucoma has not been done before.Objective: To determine the difference of malondialdehyde levels in aqueous humour between primary open-angle glaucoma and primary angle-closure glaucoma.Methods: This study was an analytical observational study. Nine subjects were primary open-angle glaucoma patients and ten subjects were primary angle-closure glaucoma patients. The aqueous humour was aspirated from the anterior chamber during trabeculectomy and measured the MDA levels using a spectrophotometer. Data were analyzed by Mann Whitney test.Results: There was no significant difference (p=0.095) of MDA levels in aqueous humour between primary open-angle glaucoma (62.913±17.259 ?mol/L) and primary angle-closure glaucoma (53.485±9.670 ?mol/L).Conclusion: The oxidative stress level in primary open-angle glaucoma and primary angle-closure glaucoma are the same, indicate that inflammation status of both condition have been similar.
The prevalence and characteristics of perineal rupture during vaginal delivery at Sanglah General Hospital and Regional Hospitals in Bali from January 2018 until December 2019 period
Authors: Megadhana I Wayan , Indrawan I Gede Suputra , Sanjaya I Nyoman Hariyasa , Aryana Made Bagus Dwi 
Published: December 2022

Background: One of the causes contributing to the high maternal mortality rate in Indonesia is postpartum hemorrhage, which can be the consequence of perineal rupture. Perineal rupture can happen spontaneously in normal vaginal delivery or worsen after episiotomy, induction of labor, vacuum and forceps delivery. This study aims to know the prevalence and characteristics of perineal rupture so that it can be used as data to make policy guidelines for preventing and reducing the incidence of perineal rupture.
Methods: This is a retrospective descriptive study analyzing secondary data collected from Sanglah General Hospital and nine other Regional Hospitals from January 2018 until December 2019. The data analysis technique used is a univariable analysis by calculating the frequency distribution and proportion to determine the prevalence and characteristics of the research subjects. Data were analyzed using SPSS version 20 for Windows.
Results: There are 8,178 cases of vaginal delivery, 6,191 cases (75.70%) with perineal rupture and 1,987 cases (24.30%) without perineal rupture. Perineal rupture is most often found in women < 20 years old (82.66%), primigravida (81.76%), preterm gestational age (78.23%), the baby with birth weight > 4,000 grams (94.26%), underweight mother (87.50%), and women with assisted delivery by vacuum or forceps (100.00%).
Conclusion: The Prevalence of perineal rupture in this study is 75.70%, with the most characteristics being women < 20 years old, primigravida, preterm gestational age, macrosomia baby, normal IMT, assisted vaginal delivery.
User interface design of Be-Health application for children's learning with a gamification approach
Authors: Wahyudi Muhammad , Destania Herwanda Ayu , Alfandi Rochmat Rizky , Sagirani Tri 
Published: December 2022

Introduction: Indonesia has the fourth-largest child population globally, estimated at 80 million children by the Central Statistics Agency (BPS). The application of Clean and Healthy Living Behavior (PHBS) in early childhood can reduce the number of early childhood health complaints in Indonesia. In addition, the role of positive parenting is also needed to keep the child's environment clean and healthy. This study aimed to determine the attractiveness, clarity, efficiency, accuracy, and novelty of the Be-Health application as a target for parents to help implement PHPBS for early childhood.
Methods: The data was taken from the Statistics Agency in 2018. The Be-Health application's implementation stage used the application development method Extreme Programming (XP). XP was chosen because it is a simple, effective, and efficient application development method. There are five stages in developing the Be-Health application, including five stages, including planning, design, coding, testing, software increment.
Results: Be-Health app is an Android-based application that provides a means to help parents apply PHBS to early childhood. Children can play and learn using the Be-Health application that applies the concept of gamification. It allows children to play and learn to complete learning missions about the application of PHBS through the Be-Health application.
Conclusion: Based on the results of the prototype design of The Be-Health application using the User Experience Questionnaire (UEQ), it can be concluded that the Be-Health application prototype has above average value for the attributes of attractiveness, clarity, efficiency, stimulation and novelty.
Increasing dental and oral health knowledge through health promotion of demonstration
Authors: Sunanto Sunanto , Handayani Erna 
Published: December 2022

Introduction: Dental and oral diseases are experienced by many children, especially kindergarten or elementary school children. Health promotion with a demonstration method with props is one of the first steps in conveying dental health knowledge and a mouth that is easy to understand by children, using a humorous approach, the demonstration method is one of the most effective methods. The purpose of the study was to determine the effect of counseling demonstration method on increasing knowledge about dental health and mouth in Children at TPQ Al Jakfar Tiris Probolinggo
Methods: Methods: this study used a pre-experimental design that used a one-group pretest-post-test approach with a design of 20 students. The type of research is used quasi-experimental with pretest-posttest design with control group design. The research subjects were divided into the treatment group (20 people) and the control group (17 people). The research sample is based on purposive sampling. Questionnaire to measure knowledge and check list to measure skills. Data analysis used paired t-test.
Results: The research shows that there is an effect of demonstration method counseling on increasing knowledge about dental and oral health in children at TPQ Al Jakfar Tiris Probolinggo. From the analysis results showed that the significant data equal to <0.001, p <α = 0,05 the result is H1 is accepted.
Conclusion: Health promotion methods with demonstration models have an effect on increasing knowledge about dental and oral health in children at TPQ Al Jakfar Tiris Probolinggo.
Change in ratio levels of KIM-1 / urine creatinine and increase serum creatinine levels in human immunodeficiency virus (HIV) patients receiving tenofovir-based antiretroviral (ARV) combination therapy
Authors: Murti I Dewa Gede Teguh Krisna , I Ketus Agus Somia  , Widiana I Gde Raka 
Published: December 2022

Background: Human immunodeficiency virus (HIV)-associated nephropathy refers to developing kidney disease associated with HIV infection. Tenofovir is associated with an increased risk of acute kidney injury (AKI). Kidney injury molecule-1 (KIM-1) in urine is very specific for kidney injury. This study aimed to determine the changes in the ratio between the levels of KIM-1/urine creatinine and the increase in serum creatinine levels with the incidence of decreased kidney function in people with HIV.
Method: This was an observational-analytic study. Subjects were taken using consecutive sampling at Sanglah Hospital Denpasar in 2018. KIM-1 levels were measured by the ELISA method.
Results: This study involved 35 patients (21 males and 14 females) with a mean age of 34.89 ± 10.05 years old. The average levels of KIM-1/urine creatinine at 0 hours was 11.039 ± 12.175 ng/ml), KIM-1/urine creatinine at 12 was 12.382 ± 14.671 ng/ml), KIM-1/urine creatinine at 72 was 10.272 ± 11.843 ng/ml). There were no statistically significant differences in the ratio of hours 0 to 12 hours (p = 0.295), and between hours 0-72 (p = 0.413). But there was a significant difference in creatinine levels for 3 months with the initial mean serum (0.848 ± 0.201) and 3 months of evaluation (1.002 ± 0.198) (p = <0.001).
Conclusion: There was no difference in the ratio of changes in serum KIM-1/creatinine levels during the evaluation of 0, 12, and 72 hours. However, there was an increase in serum creatinine levels between the beginning and 3 months of evaluation.
Relationship between CD4 levels and mucocutaneous manifestations in HIV-AIDS patients at Dr. Soetomo General Academic Teaching Hospital, Surabaya, Indonesia
Authors: Citra Dwi Harningtyas  , Damayanti  , Maylita Sari  , Muhammad Yulianto Listiawan  , Diah Mira Indramaya  , Linda Astari  , Budi Utomo  , Dwi Murtiastutik  , Setyana Widyantari  , Astindari  , Afif Nurul Hidayati  
Published: December 2022

Background: CD4 can assess the immune status of a patient with HIV, and this is considered the standard way of assessing and characterizing the severity of HIV-related immunodeficiency. CD4 cell counts are associated with increased mucocutaneous manifestations. This study aims to explain the relationship between CD4 and mucocutaneous manifestations in HIV-AIDS patients.
Methods: This study is analytical with a retrospective cross-sectional design. The data of the present study was obtained from a number of 614 HIV-AIDS patients with mucocutaneous manifestations, but only 149 patients met the inclusion criteria, including the CD4 level data.
Results: The majority of the patients in this study were male (74.5%), which includes patients under the age group of 25-49 years (70.5%). The most common risk factor was heterosexuality (48.3%). Most CD4 levels were in the CD4 group < 200 (64.4%). Based on the distribution of mucocutaneous manifestations, the various cases were reported as infections (55.8%). Pyoderma and syphilis were significantly associated with CD4 counts ≥ 200 cells/mm3 with a risk of 3.7 and 7.8 times than CD4 cells < 200 cells / mm3. On the other hand, candidiasis was higher in CD4 count < 200 cells/mm3 with a significant difference and a risk of 0.3 times compared to CD4 count ≥ 200 cells/mm3.
Conclusion: Several mucocutaneous manifestations can be considered predictors of advanced immunosuppression (low CD4 level), which were pyoderma, syphilis, and candidiasis in this study.
The potential of carrot extract as a sunscreen to prevent apoptosis in white mice (Mus musculus) fibroblast cell cultures exposed to UVB light
Authors: Bagus Komang Satriyasa  , I Gusti Ayu Widianti  , I.B.G. Fajar Manuaba  
Published: December 2022

Introduction: UVB rays are one of the main external factors that play a role in the aging process. Apoptosis of fibroblasts affects skin aging, and the decline in fibroblasts is also caused by the reduced number and ability of growth factors and other hormones. Sunscreen has been used for a long time to protect the skin from the harmful effects of solar radiation, especially UVB rays. Antioxidant activity such as β-carotene and vitamin E from carrots can counteract free radicals and oxidative stress caused by UV radiation. This study aims to test whether in vitro administration of carrot extract can be used as a sunscreen to prevent apoptosis in mouse fibroblast cell cultures exposed to UVB light.
Method: This research is a laboratory experimental study, using a Randomized Post-test Only Control Group Design. The research sample was fibroblast cell culture derived from the back skin of white mice (Mus Musculus) isolated with Collagenase 1 (primary culture). Observation of fibroblast apoptosis using propidium iodide staining. Data were analyzed using Shapiro-Wilk test, Levene's test, One Way Anova and continued with LSD test. The confidence level in this study is 95%.
Result: There was a significant difference between the control group and the carrot extract treatment group, but no significant difference was found between the SPF15 group and the carrot group (p>0.01).
Conclusion: Carrot extract can provide a protective effect on the skin (fibroblasts) and prevent apoptosis.
The effect of kefir polysaccharide extract on the expression of matrix metalloproteinase 9 (MMP-9) and integrin activation of HeLa cervical carcinoma culture cell
Authors: Yahya Irwanto  , Eddy Mustofa  , Teguh Wiyono  , Dodi Tri Oktafianto  , Arif Rahman Nurdianto  , Robby Rinaldi Widodo  
Published: December 2022

Background: Cervical cancer is the leading cause of cancer in the world, especially in developing countries. Kefir is a goat's milk product that contains a lot of nutrients and bioactive complex which are involved in the synthesis of the anticancer, and have anticarcinogenic and antimutagenic effect. Integrins are involved in various biological processes associated with cancer development, such as migration, invasion, differentiation and proliferation. MMP-9 can also degrade ECM through proteolytic action, affecting cell-cell interactions in ECM. This research aimed to see the effect of kefir on MMP-9 and integrins activation. Thus, it is expected that kefir can be used as an additional supplement in patients with cervical cancer.
Methods: This research study was conducted in an experimental laboratory in vitro using HeLa cells as the object of research. Data were analyzed using the SPSS program to determine the most effective dose of kefir and a regression test to determine the effect between integrins and MMP-9.
Results: The mean value of MMP-9 and integrin at a dose of 180 µg/ml was 165.1 ± 5.8 and 378.5 ± 12.6 respectively. This result had a significant value of p = 0.0000 for both MMP-9 and integrin-b. In the regression test, the value of p = 0.05 was obtained while the coefficient value was positive (0.565).
Conclusion: The higher the expression of MMP-9, the activation of the integrin-b would increase. Kefir extract dosage of 180 µg/ml significantly reduced MMP-9 and Integrin-b expression. MMP-9 affects improving Integrin-b