bluuebunny/crossref_metadata_2025_split huggingface dataset:
ARID3A promotes the chemosensitivity of colon cancer by inhibiting AKR1C3
Authors: Li Yafei , Tang Jing , Li Jing , Du Yaru , Bai Fuqiang , Yang Lirui , Li Xiaobo , Jin Xiaoming , Wang Tianzhen 
Published: March 2022

AbstractARID3A is upregulated in colorectal cancer and can promote the proliferation and metastasis of cancer cells. However, patients with higher level of ARID3A have a better prognosis. This study aimed to uncover the mechanism by which ARID3A benefits the prognosis of colon cancer. Our results indicated that ARID3A upregulation enhanced the chemosensitivity of colon cancer cells to 5‐fluorouracil (5‐FU), whereas ARID3A downregulation inhibited the chemosensitivity of colon cancer cells to 5‐FU. Through database analysis, we found that AKR1C3, a drug resistance‐related gene, was the target of ARID3A. Moreover, AKR1C3 was downregulated in colon cancer tissues compared to normal tissues. Next, we assessed the interaction between AKR1C3 and ARID3A, and found that ARID3A inhibited the transcription of AKR1C3, leading to the downregulation of AKR1C3 in colon cancer cells. We also verified that AKR1C3 inhibited the chemosensitivity of colon cancer cells to 5‐FU. Moreover, patients with higher ratio of ARID3A to AKR1C3 had a better prognosis. This study suggested that ARID3A promoted chemosensitivity of colon cancer cells by inhibiting AKR1C3 in colon cancer. The ratio of ARID3A to AKR1C3 is a good marker to predict the prognosis of colon cancer patients.
Controllable Intercalated Polyaniline Nanofibers Highly Enhancing the Utilization of Delaminated RuO2 Nanosheets for High‐Performance Hybrid Supercapacitors
Authors: Zhang Gaini , Deng Lingjuan , Liu Jinqian , Zhang Jianhua , Wang Jingjing , Li Wenbin , Li Xifei 
Published: March 2022

AbstractRuthenium oxide (RuO2) and polyaniline (PANI) are attracting extensive attention as electrode materials due to their remarkable pseudocapacitance. However, RuO2 suffers from low utilization and PANI sustains poor stability. Herein, both defects were optimized by designing intercalated‐structure PANI/RuO2 composites, which were fabricated for the first time by controllable chemical polymerization of aniline on the delaminated RuO2 nanosheets. Intercalated PANI nanofibers not only highly prevent the RuO2 nanosheets from their reassembling as well as increase the interlayer spacing among RuO2 nanosheets but also alleviate the swelling/shrinking of PANI during charge‐discharge progress. In comparison to the pristine RuO2 nanosheets, the obtained intercalated structure is beneficial for creating channels for better ion transfer and electron transport, which leads to a high‐efficiency utilization of RuO2 nanosheets. The optimized PANI/RuO2‐38 electrode with a RuO2 content of 61.7 wt% shows the highest utilization of 72.1 % of RuO2, resulting in the highest specific capacity of 816 F g−1. The asymmetric supercapacitor PANI/RuO2//AC offers a high energy density of 25.7 Wh kg−1 at 375 kW kg−1, further indicating a good potential as energy‐storage device.
Polycations Stabilised by Borosulfates: [Au3Cl4][B(S2O7)2] and the One‐Dimensional Metal [Au2Cl4][B(S2O7)2](SO3)
Authors: Sutorius Stefan , van Gerven David , Olthof Selina , Rasche Bertold , Bruns Jörn 
Published: March 2022

Abstract(SO4)‐rich silicate analogue borosulfates are able to stabilise cationic cluster‐like and chain‐like aggregates. Single crystals of [Au3Cl4][B(S2O7)2] and [Au2Cl4][B(S2O7)2](SO3) were obtained by solvothermal reaction with SO3, and the electronic properties were investigated by means of density functional theory–based calculations. [Au3Cl4][B(S2O7)2] exhibits a cluster‐like cation, and the cationic gold‐chloride strands in [Au2Cl4][B(S2O7)2](SO3) are found to resemble one‐dimensional metallic wires. This is confirmed by polarisation microscopy.
The impact of whole genome and transcriptome analysis (WGTA) on predictive biomarker discovery and diagnostic accuracy of advanced malignancies
Authors: Tessier‐Cloutier Basile , Grewal Jasleen K , Jones Martin R , Pleasance Erin , Shen Yaoqing , Cai Ellen , Dunham Chris , Hoang Lynn , Horst Basil , Huntsman David G , Ionescu Diana , Karnezis Anthony N , Lee Anna F , Lee Cheng Han , Lee Tae Hoon , Twa David DW , Mungall Andrew J , Mungall Karen , Naso Julia R , Ng Tony , Schaeffer David F , Sheffield Brandon S , Skinnider Brian , Smith Tyler , Williamson Laura , Zhong Ellia , Regier Dean A , Laskin Janessa , Marra Marco A , Gilks C Blake , Jones Steven JM , Yip Stephen 
Published: March 2022

AbstractIn this study, we evaluate the impact of whole genome and transcriptome analysis (WGTA) on predictive molecular profiling and histologic diagnosis in a cohort of advanced malignancies. WGTA was used to generate reports including molecular alterations and site/tissue of origin prediction. Two reviewers analyzed genomic reports, clinical history, and tumor pathology. We used National Comprehensive Cancer Network (NCCN) consensus guidelines, Food and Drug Administration (FDA) approvals, and provincially reimbursed treatments to define genomic biomarkers associated with approved targeted therapeutic options (TTOs). Tumor tissue/site of origin was reassessed for most cases using genomic analysis, including a machine learning algorithm (Supervised Cancer Origin Prediction Using Expression [SCOPE]) trained on The Cancer Genome Atlas data. WGTA was performed on 652 cases, including a range of primary tumor types/tumor sites and 15 malignant tumors of uncertain histogenesis (MTUH). At the time WGTA was performed, alterations associated with an approved TTO were identified in 39 (6%) cases; 3 of these were not identified through routine pathology workup. In seven (1%) cases, the pathology workup either failed, was not performed, or gave a different result from the WGTA. Approved TTOs identified by WGTA increased to 103 (16%) when applying 2021 guidelines. The histopathologic diagnosis was reviewed in 389 cases and agreed with the diagnostic consensus after WGTA in 94% of non‐MTUH cases (n = 374). The remainder included situations where the morphologic diagnosis was changed based on WGTA and clinical data (0.5%), or where the WGTA was non‐contributory (5%). The 15 MTUH were all diagnosed as specific tumor types by WGTA. Tumor board reviews including WGTA agreed with almost all initial predictive molecular profile and histopathologic diagnoses. WGTA was a powerful tool to assign site/tissue of origin in MTUH. Current efforts focus on improving therapeutic predictive power and decreasing cost to enhance use of WGTA data as a routine clinical test.
An Assessment of Zinc Fluxes by Analyzing Monthly, Weekday, and Weekend Levels in a River
Authors: Andarani Pertiwi , Yokota Kuriko , Inoue Takanobu , Alimuddin Hardianti , Nguyen Minh Ngoc 
Published: March 2022

AbstractUnlike other potentially toxic metals, zinc (Zn) is indispensable to life but also poses environmental risks to aquatic organisms. Aichi Prefecture has the Japan's fourth‐highest discharges of Zn into water bodies. It is likely that the Zn fluxes in Aichi's water bodies originate from industrial wastewater. This study evaluates the spatial‐temporal and diel variability of Zn on sunny days during weekdays and weekends in the Umeda River. The most downstream point is considered as the most polluted section according to the monthly survey [dissolved Zn (D‐Zn): 0.0046–0.0719 mg L−1, particulate Zn (P‐Zn): 0.42–2.01 mg g−1] that varied between seasons (coefficient of variation: 95% for D‐Zn; 53% for P‐Zn). The total Zn (T‐Zn) concentrations on weekdays (0.015–0.043 mg L−1) at the most downstream point exhibit much higher concentrations than those during the weekends (undetected–0.032 mg L−1). Given the dissolved phase of these Zn levels (77 ± 11%), the Zn are likely discharged by industrial facilities on weekdays. The T‐Zn loading on weekdays (56 g km−2 d−1) is approximately three times higher than that on weekends. At least 67% of the T‐Zn and 70% of the D‐Zn fluxes from industrial point sources are potentially discharged on weekdays.
Highly Stable Nanostructured Magnetic Vesicles as Doxorubicin Carriers for Field‐assisted Therapies
Authors: Andrada Heber E. , Venosta Lisandro , Jacobo Silvia E. , Fernando Silva O. , Darío Falcone R. , Bercoff Paula G. 
Published: March 2022

AbstractIn this work, magnetic vesicles were produced using magnetite (Fe3O4) nanoparticles and 1‐methylimidazolium bis‐ (2‐ethylhexyl) phosphate (imim‐DEHP) as surfactant. Some of the vesicles were subsequently coated with chitosan to improve their stability and biocompatibility. A physicochemical characterization of the prepared systems was carried out using several techniques, which allowed verifying that the magnetic nanoparticles were successfully encapsulated. Finally, the vesicles were loaded with an antitumor drug (doxorubicin) by swelling in order to perform release studies. The Korsmeyer‐Peppas model was used to fit the doxorubicin (Dox) release profiles in the different vesicle systems, which displayed delayed release in every case. After 24 h, the Dox release percentages ranged between 43–53%. The prepared vesicles show not only excellent stability but also a good response to an external magnetic field, which makes them good candidates to be used in field‐assisted therapies, thus trying to improve therapeutic efficacy and avoiding possible adverse effects.
Nature‐inspired virtual machine placement mechanisms: A systematic review
Authors: Kong Yuqiang , He Yaoping , Abnoosian Karlo 
Published: March 2022

AbstractCloud data centers do not completely use their resources, resulting in resource underutilization. Cloud computing companies primarily leverage virtualization technologies to supply cost‐effective service provision. In order to optimize cloud performance, virtual machines (VMs) must be placed among physical machines (PMs). When it comes to concentrating on the issues in the cloud computing environment, effective VM placement (VMP) is one of the primary difficulties that might cost suppliers money. VMP may be applied in a variety of ways in cloud computing. In terms of lowering related processing overhead, consolidating the cloud environment to become a highly on‐demand method, balancing the load among PMs, power usage, and refining performance, VMP techniques still require improvement in the computing environment. This study aims to provide a comprehensive overview of VMP approaches. This article provides an up‐to‐date survey of the most related VMP literature to highlight study possibilities in cloud settings utilizing nature‐inspired metaheuristic algorithms. The findings suggest that placing VMs in the most efficient place saves power usage substantially. The key problem is to minimize data center energy usage without compromising performance or breaking service level agreements. Finally, we will discuss and look at what further may be accomplished in this line of science.
A multi‐agent based mechanism for collaboratively detecting distributed denial of service attacks in internet of vehicles
Authors: Dong Tingting , Chen Lei , Zhou Li , Xue Fei , Qin Huilin 
Published: March 2022

AbstractDistributed denial of service (DDoS) attacks have become a hidden danger in the development of the internet of vehicles (IoV). DDoS attacks for TCP protocol are studied to improve the information security environment of IoV. For the distribution characteristics of DDoS attacks, an information sharing and collaborative detection mechanism based on multi‐agent is proposed. Considering the relationship between the features of adjacent moments in the TCP communication, the DDoS detection model based on hidden Markov model is built, and the Viterbi algorithm is improved for the problem of the false alarm in the observation sequence. The optimal communication strategy among agents is determined by deep reinforcement learning, and fusion algorithm is designed to improve the current strategy of agents. Three groups of comparative experiments are designed and analyzed. The simulation results show that proposed algorithms are effective.
Identifying Antidepressants Less Likely to Cause Hyponatremia: Triangulation of Retrospective Cohort, Disproportionality, and Pharmacodynamic Studies
Authors: Nagashima Takuya , Hayakawa Takashi , Akimoto Hayato , Minagawa Kimino , Takahashi Yasuo , Asai Satoshi 
Published: March 2022

Antidepressants are known to cause hyponatremia, but conflicting evidence exists regarding specific antidepressants. To identify antidepressants less likely to cause hyponatremia, we conducted a triangulation study integrating retrospective cohort, disproportionality, and pharmacodynamic studies. In the retrospective cohort study of patients (≥ 60 years) in Nihon University School of Medicine’s Clinical Data Warehouse (2004–2020), a significant decrease in serum sodium levels was observed within 30 days after initiation of a selective serotonin reuptake inhibitor (SSRI; mean change −1.00 ± 0.23 mmol/L, P < 0.001) or serotonin‐noradrenaline reuptake inhibitor (SNRI; −1.01 ± 0.31 mmol/L, P = 0.0013), whereas no decrease was found for a noradrenergic and specific serotonergic antidepressant (mirtazapine; +0.55 ± 0.47 mmol/L, P = 0.24). Within‐class comparison revealed no decrease in serum sodium levels for fluvoxamine (+0.74 ± 0.75 mmol/L, P = 0.33) among SSRIs and milnacipran (+0.08 ± 0.87 mmol/L, P = 0.93) among SNRIs. In the disproportionality analysis of patients (≥ 60 years) in the Japanese Adverse Drug Event Report database (2004–2020), a significant increase in hyponatremia reports was observed for SSRIs (reporting odds ratio 4.41, 95% confidence interval 3.58–5.45) and SNRIs (5.66, 4.38–7.31), but not for mirtazapine (1.08, 0.74–1.58), fluvoxamine (1.48, 0.94–2.32), and milnacipran (0.85, 0.45–1.62). Finally, pharmacoepidemiological–pharmacodynamic analysis revealed a significant correlation between the decrease in serum sodium levels and binding affinity for serotonin transporter (SERT; r = −0.84, P = 0.02), suggesting that lower binding affinity of mirtazapine, fluvoxamine, and milnacipran against SERT is responsible for the above difference. Although further research is needed, our data suggest that mirtazapine, fluvoxamine, and milnacipran are less likely to cause hyponatremia.
Vericiguat in Combination with Short‐Acting Nitroglycerin in Patients With Chronic Coronary Syndromes: The Randomized, Phase Ib, VENICE Study
Authors: Boettcher Michael , Düngen Hans‐Dirk , Donath Frank , Mikus Gerd , Werner Nikos , Thuermann Petra A. , Karakas Mahir , Besche Nina , Koch Tanja , Gurniak Matthias , Becker Corina 
Published: March 2022

Vericiguat is a soluble guanylate cyclase stimulator indicated to reduce the risk of cardiovascular death and heart failure (HF) hospitalization in adults with symptomatic chronic HF and ejection fraction less than 45%. Guidelines recommend short‐acting nitrates, such as sublingual nitroglycerin, for the treatment of acute angina pectoris in patients with chronic coronary syndromes (CCSs), common comorbidities in HF. We evaluated safety, tolerability, and the pharmacodynamic interaction between vericiguat and nitroglycerin, coadministered in patients with CCSs. In this phase Ib, double‐blind, randomized, multicenter study, 36 patients with CCSs received either vericiguat 2.5 mg (up‐titrated every 2 weeks to 5 mg and 10 mg) or placebo. Patients also received nitroglycerin (0.4 mg sublingual). In total, 31 patients completed the study (vericiguat + nitroglycerin, n = 21; placebo + nitroglycerin, n = 10). There was no increase in treatment‐emergent adverse events (TEAEs) with vericiguat + nitroglycerin vs. placebo + nitroglycerin; three patients discontinued due to TEAEs (vericiguat + nitroglycerin, n = 1; placebo + nitroglycerin, n = 2). Decreases in mean blood pressure (BP; 6–10 mmHg systolic BP (SBP); 4–6 mmHg diastolic BP (DBP)) were independent of vericiguat exposure and occurred to a similar extent at trough and peak concentrations with all vericiguat doses and placebo. Coadministration of vericiguat with nitroglycerin in patients with CCSs was well tolerated, and the combination is unlikely to cause significant adverse effects beyond those known for nitroglycerin.
Measurable Residual Disease Detection in B‐Acute Lymphoblastic Leukemia: The Children's Oncology Group (COG) Method
Authors: Borowitz Michael J. , Wood Brent L. , Keeney Michael , Hedley Benjamin D. 
Published: March 2022

AbstractMeasurable (minimal) residual disease (MRD) in B‐acute lymphoblastic leukemia (B‐ALL), as assessed by flow cytometry, is an established prognostic factor used to adjust treatment in most pediatric therapeutic protocols. MRD in B‐ALL has been standardized by the Children's Oncology Group in North America and more recently in a multicenter Foundation for the National Institutes of Health–funded study. This article outlines the reagents, instrument setup, and analysis protocols required for the reproducible detection of residual leukemic cells in patients following induction therapy for B‐ALL. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Staining and flow cytometry for B‐acute lymphoblastic leukemia (B‐ALL) measurable residual disease detectionSupport Protocol: Specimen collection, handling, storage, and shippingBasic Protocol 2: Analysis and interpretation of data for B‐ALL measurable residual disease detectionBasic Protocol 3: Analysis of samples lacking sufficient CD19+ events
Preparation and Use of Cellular Reagents: A Low‐resource Molecular Biology Reagent Platform
Authors: Bhadra Sanchita , Paik Inyup , Torres Jose‐Angel , Fadanka Stéphane , Gandini Chiara , Akligoh Harry , Molloy Jenny , Ellington Andrew D. 
Published: March 2022

AbstractProtein reagents are indispensable for most molecular and synthetic biology procedures. Most conventional protocols rely on highly purified protein reagents that require considerable expertise, time, and infrastructure to produce. In consequence, most proteins are acquired from commercial sources, reagent expense is often high, and accessibility may be hampered by shipping delays, customs barriers, geopolitical constraints, and the need for a constant cold chain. Such limitations to the widespread availability of protein reagents, in turn, limit the expansion and adoption of molecular biology methods in research, education, and technology development and application. Here, we describe protocols for producing a low‐resource and locally sustainable reagent delivery system, termed “cellular reagents,” in which bacteria engineered to overexpress proteins of interest are dried and can then be used directly as reagent packets in numerous molecular biology reactions, without the need for protein purification or a constant cold chain. As an example of their application, we describe the execution of polymerase chain reaction (PCR) and loop‐mediated isothermal amplification (LAMP) using cellular reagents, detailing how to replace pure protein reagents with optimal amounts of rehydrated cellular reagents. We additionally describe a do‐it‐yourself fluorescence visualization device for using these cellular reagents in common molecular biology applications. The methods presented in this article can be used for low‐cost, on‐site production of commonly used molecular biology reagents (including DNA and RNA polymerases, reverse transcriptases, and ligases) with minimal instrumentation and expertise, and without the need for protein purification. Consequently, these methods should generally make molecular biology reagents more affordable and accessible. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Preparation of cellular reagentsAlternate Protocol 1: Preparation of lyophilized cellular reagentsAlternate Protocol 2: Evaluation of bacterial culture growth via comparison to McFarland turbidity standardsSupport Protocol 1: SDS‐PAGE for protein expression analysis of cellular reagentsBasic Protocol 2: Using Taq DNA polymerase cellular reagents for PCRBasic Protocol 3: Using Br512 DNA polymerase cellular reagents for loop‐mediated isothermal amplification (LAMP)Support Protocol 2: Building a fluorescence visualization device
Generation of Complex Syngeneic Liver Organoids from Induced Pluripotent Stem Cells to Model Human Liver Pathophysiology
Authors: Raggi Claudia , Selleri Silvia , M'Callum Marie‐Agnes , Paganelli Massimiliano 
Published: March 2022

AbstractThe study of human liver pathophysiology has been hampered for decades by the lack of easily accessible, robust, and representative in vitro models. The discovery of induced pluripotent stem cells (iPSCs)—which can be generated from patients’ somatic cells, engineered to harbor specific mutations, and differentiated into hepatocyte‐like cells—opened the way to more meaningful modeling of liver development and disease. Nevertheless, representative modeling of many complex liver conditions requires the recreation of the interplay between hepatocytes and nonparenchymal liver cells. Here we describe protocols we developed to generate and characterize complex human liver organoids composed of iPSC‐derived hepatic, endothelial, and mesenchymal cells. With all cell types derived from the same iPSC population, such organoids reproduce the liver niche, allowing for the study of liver development and the modeling of complex inflammatory and fibrotic conditions. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Differentiation of human iPSCs into hepatic progenitor cells (hepatoblasts)Basic Protocol 2: Differentiation of human iPSCs into endothelial progenitor cellsSupport Protocol 1: Characterization of iPSC‐derived endothelial progenitor cellsBasic Protocol 3: Differentiation of human iPSCs into mesenchymal progenitor cellsSupport Protocol 2: Characterization of iPSC‐derived mesenchymal progenitor cellsBasic Protocol 4: Generation of complex syngeneic liver organoids
Specialist supportive clinical management for anorexia nervosa: Analysis of therapy content and relation to outcome
Authors: McIntosh Virginia Violet Williams , Carter Janet Deborah , Jordan Jennifer , Loughlin Alex 
Published: March 2022

AbstractObjectivePsychotherapy for anorexia nervosa (AN) is complex and multifaceted, with little known about likely effective components of treatments. The current study explored the spoken content of specialist supportive clinical management (SSCM) for AN, a treatment with evidence of effectiveness in several randomized clinical trials.MethodOne hundred seventy‐eight therapy sessions constituting all ten therapist–patient dyads of those who completed SSCM treatment in the original clinical trial of SSCM, were transcribed verbatim. Themes were developed and content analyzed using qualitative content analysis of complete therapy sessions by four analysts, with 10% of sessions cocoded for interrater agreement.ResultsOver three quarters of session content was within the clinical management theme, the largest subtheme relating to normalizing eating, followed by weight, mechanics of SSCM, and encouragement of self‐care. Approximately 20% of total content was in the supportive psychotherapy theme, half about relationships. The relative proportion of clinical management content remained high, decreasing during the last five sessions. Those achieving good outcome did not have a lower ratio of clinical management to supportive psychotherapy content.DiscussionThe current study revealed strong clinical management focus on core symptoms of AN—normalization of eating and weight gain—throughout SSCM for AN.Public SignificanceSix clinical trials have found SSCM to be effective in treating AN. Content of SSCM sessions in the original trial was classified using qualitative content analysis. Study findings revealed strong clinical management focus on core AN symptoms—normalization of eating and weight gain—throughout SSCM, with the ratio of clinical management to supportive psychotherapy greater for those with a good outcome.