bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of MiR‐100‐5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo
Authors: Ma Li , Zhang Meng , Cao Fangjun , Han Jincheng , Han Peng , Wu Yeting , Deng Renyi , Zhang Guanghui , An Xiaopeng , Zhang Lei , Song Yuxuan , Cao Binyun 
Published: April 2022

AbstractThe growth of endometrial stromal cells (ESCs) at implantation sites may be a potential factor affecting the success rate of embryo implantation. Incremental proofs demonstrated that ncRNAs (e.g. miRNAs, lncRNAs and circRNAs) were involved in various biological procedures, including proliferation and apoptosis. In this study, the role of miR‐100‐5p on proliferation and apoptosis of goat ESCs in vitro and embryo implantation in vivo was determined. The mRNA expression of miR‐100‐5p was significantly inhibited in the receptive phase (RE) rather than in the pre‐receptive phase (PE). Overexpression of miR‐100‐5p suppressed ESCs proliferation and induced apoptosis. The molecular target of MiR‐100‐5p, HOXA1, was confirmed by 3′‐UTR assays. Meanwhile, the product of HOXA1 mRNA RT‐PCR increased in the RE more than that in the PE. The HOXA1‐siRNA exerted significant negative effects on growth arrest. Instead, incubation of ESCs with miR‐100‐5p inhibitor or overexpressed HOXA1 promoted the cell proliferation. In addition, Circ‐9110 which acted as a sponge for miR‐100‐5p reversed the relevant biological effects of miR‐100‐5p. The intrinsic apoptosis pathway was suppressed in ESCs, revealing a crosstalk between Circ‐9110/miR‐100‐5p/HOXA1 axis, PI3K/AKT/mTOR, and ERK1/2 pathways. To further evaluate the progress in study on embryo implantation regulating mechanism of miR‐100‐5p in vivo, the pinopodes of two phases were observed and analysed, suggesting that, as similar as in situ, miR‐100‐5p was involved in significantly regulating embryo implantation in vivo. Mechanistically, miR‐100‐5p performed its embryo implantation function through regulation of PI3K/AKT/mTOR and ERK1/2 pathways by targeting Circ‐9110/miR‐100‐5p/HOXA1 axis in vivo.
Quality and clinical applicability of recommendations for incontinence‐associated dermatitis: A systematic review of guidelines and consensus statements
Authors: Chen Yamin , Gao Ya , Zhang Jiaoyan , Niu Mingming , Liu Xiaofeng , Zhang Yuqin , Tian Jinhui 
Published: April 2022

AbstractAims and ObjectivesThe aim of this study was to assess methodological quality of all currently available guidelines and consensus statements for IAD using the Appraisal of Guidelines, Research and Evaluation (AGREE) II and the AGREE Recommendation Excellence (AGREE‐REX) instruments.BackgroundGlobally, incontinence‐associated dermatitis (IAD) is a significant health challenge. IAD is a complex healthcare problem that reduces quality of life of patients, increases healthcare costs and prolongs hospital stays. Several guidelines and consensus statements are available for IAD. However, the quality of these guidelines and consensus statements remains unclear.DesignA systematic review of guidelines and consensus statements.MethodsOur study was undertaken using PRISMA guidelines. We searched seven electronic databases. Guidelines and consensus statements had to be published in English, Chinese or German languages. Five independent reviewers assessed the methodological quality of guidelines and consensus statements using the AGREE II and AGREE‐REX instruments. Mean with standard deviation (SD) and median with interquartile range (IQR) were calculated for descriptive analyses. We generated bubble plots to describe the assessment results of each domain of each guideline and consensus statement.ResultsWe included ten guidelines and consensus statements. The NICE guidelines, obtained the highest scores, fulfilled 86.11%–98.61% of criteria in AGREE II and 76.67%–91.11% for AGREE‐REX. In the domains ‘Stakeholder Involvement’ (4.39 ± 1.64), ‘Rigor of Development’ (3.38 ± 1.86), ‘Applicability’ (3.62 ± 1.64), ‘Editorial Independence’ (3.91 ± 2.56) and ‘Values and Preferences’ (2.98 ± 1.41), the remaining guidelines and consensus statements showed deficiencies.ConclusionsAltogether, this study demonstrated that the currently available guidelines and consensus statements for IAD have room for methodological improvement. NICE guidelines on faecal incontinence and urinary incontinence have better quality. Remaining guidelines and consensus statements showed substantial methodological weaknesses, especially the domains of ‘Stakeholder Involvement’, ‘Rigor of Development’, ‘Applicability’, ‘Editorial independence’ and ‘Values and Preferences’. This study was registered on INPLASY. (Registration number: INPLASY202190078).Relevance to Clinical PracticeThe currently available guidelines and consensus statements on IAD have room for methodological improvement.
COVID‐19 in Latin America and the Caribbean: Two years of the pandemic
Authors: Schwalb Alvaro , Armyra Eleonora , Méndez‐Aranda Melissa , Ugarte‐Gil César 
Published: April 2022

AbstractWorldwide, nations have struggled during the coronavirus disease 2019 (COVID‐19) pandemic. However, Latin America and the Caribbean faced an unmatched catastrophic toll. As of March 2022, the region has reported approximately 15% of cases and 28% of deaths worldwide. Considering the relatively late arrival of SARS‐CoV‐2, several factors in the region were determinants of the humanitarian crisis that ensued. Pandemic unpreparedness, fragile healthcare systems, forthright inequalities, and poor governmental support facilitated the spread of the virus throughout the region. Moreover, reliance on repurposed and ineffective drugs such as hydroxychloroquine and ivermectin—to treat or prevent COVID‐19—was publicised through misinformation and created a false sense of security and poor adherence to social distancing measures. While there were hopes that herd immunity could be achieved after the region's disastrous first peak, the emergence of the Gamma, Lambda, and Mu variants made this unattainable. This review explores how Latin America and the Caribbean fared during the first 2 years of the pandemic, and how, despite all the challenges, the region became a global leader in COVID‐19 vaccination, with 63% of its population fully vaccinated.
Effects of Remote Education During the COVID‐19 Pandemic on Young Children's Learning and Academic Behavior in Georgia: Perceptions of Parents and School Administrators
Authors: Klosky Jill V. , Gazmararian Julie A. , Casimir Olivia , Blake Sarah C. 
Published: April 2022

ABSTRACTBACKGROUNDIn Spring 2020, Georgia public schools implemented remote learning to manage the spread of COVID‐19. This study explores the effects of remote schooling on the learning of young children in Georgia during the early COVID‐19 pandemic from the perspectives of school administrators and essential working parents.METHODSA qualitative exploratory study was conducted with eight school administrators and 26 essential working parents of children in kindergarten through third grades of two rural and two urban schools in Georgia. Data collection included online surveys, virtual interviews and focus groups. Descriptive analyses of the demographics provided context to emerging themes from qualitative data.RESULTSMost school administrators and parents reported declines in student learning and academic behavior related to remote learning. Lack of Wi‐Fi, technology, and digital literacy were often cited as barriers to learning. Challenges with remote learning were amplified for students and parents of vulnerable groups.CONCLUSIONSThe findings of this study illustrate the need to institute policies, procedures, and supports to maximize schools' ability to safely offer in‐person learning during the COVID‐19 pandemic. Considerations should be made of the needs of essential working parents, vulnerable populations, and the digital divide.
Age‐adjusted mortality and predictive value of liver chemistries in a Viennese cohort of COVID‐19 patients
Authors: Hartl Lukas , Haslinger Katharina , Angerer Martin , Jachs Mathias , Simbrunner Benedikt , Bauer David J. M. , Semmler Georg , Scheiner Bernhard , Eigenbauer Ernst , Strassl Robert , Breuer Monika , Kimberger Oliver , Laxar Daniel , Trauner Michael , Mandorfer Mattias , Reiberger Thomas 
Published: April 2022

AbstractBackground and AimsThe coronavirus disease of 2019 (COVID‐19) causes considerable mortality worldwide. We aimed to investigate the frequency and predictive role of abnormal liver chemistries in different age groups.MethodsPatients with positive severe acute respiratory distress syndrome‐coronavirus‐2 (SARS‐CoV‐2) polymerase chain reaction (PCR) test between 03/2020‐07/2021 at the Vienna General Hospital were included. Patients were stratified for age: 18–39 vs. 40–69 vs. ≥70 years (y). Aspartate aminotransferase (AST), alanine‐aminotransferase (ALT), alkaline phosphatase (ALP), gamma‐glutamyl transferase (GGT) and total bilirubin (BIL) were recorded.Results900 patients (18–39 years: 32.2%, 40–69 years: 39.7%, ≥70 years: 28.1%) were included. Number of comorbidities, median D‐dimer and C‐reactive protein increased with age. During COVID‐19, AST/ALT and ALP/GGT levels significantly increased. Elevated hepatocellular transaminases (AST/ALT) and cholestasis parameters (ALP/GGT/BIL) were observed in 40.3% (n = 262/650) and 45.0% (n = 287/638) of patients respectively. Liver‐related mortality was highest among patients with pre‐existing decompensated liver disease (28.6%, p < .001). 1.7% of patients without pre‐existing liver disease died of liver‐related causes, that is consequences of hepatic dysfunction or acute liver failure. Importantly, COVID‐19‐associated liver injury (16.0%, p < .001), abnormal liver chemistries and liver‐related mortality (6.5%, p < .001) were most frequent among 40–69 years old patients. Elevated AST and BIL after the first positive SARS‐CoV‐2 PCR independently predicted mortality in the overall cohort and in 40–69 years old patients.ConclusionsAlmost half of the COVID‐19 patients exhibit abnormal hepatocellular and cholestasis‐related liver chemistries with 40–69 years old patients being at particularly high risk for COVID‐19‐related liver injury and liver‐related mortality. Elevated AST and BIL after SARS‐CoV‐2 infection are independent predictors of mortality, especially in patients aged 40–69 years.
Effect of liquid and solid test meals on symptoms and gastric myoelectrical activity in patients with gastroparesis and functional dyspepsia
Authors: Koch Kenneth L. , Van Natta Mark , Parkman Henry P. , Grover Madhusudan , Abell Thomas L. , McCallum Richard W. , Shaltout Hossam A. , Sarosiek Irene , Farrugia Gianrico , Shulman Robert J. , Tonascia James , Miriel Laura , Hamilton Frank , Pasricha Pankaj J. ,   Gastroparesis Clinical Research Consortium
Published: April 2022

AbstractBackgroundPatients with gastroparesis (GP) and functional dyspepsia (FD) have similar symptoms, but the pathophysiology of postprandial symptoms remains uncertain.AimsTo compare symptoms and gastric myoelectrical activity (GMA) after liquid and solid test meals in patients with GP and FD.MethodsPatients enrolled in the Gastroparesis Clinical Research Consortium Registry were studied. Clinical characteristics were measured with standard questionnaires. GP was determined by 4‐h solid‐phase gastric scintigraphy. GMA was measured using electrogastrography before and after ingestion of a water load or nutrient bar on separate days. Symptoms were measured on visual analog scales. GMA responses to the water load for individual patients were also determined.Results284 patients with GP and 113 with FD were identified who ingested both test meals. Patients with GP and FD had similar maximal tolerated volumes of water [mean (SD) 378 (218) ml vs. 402 (226) ml,p = 0.23] and reported similar intensity of fullness, nausea, bloating, and abdominal discomfort after the test meals. Twenty‐six percent and 19% of the patients with GP and FD, respectively, ingested subthreshold (<238 ml) volumes of water (p = 0.15). Gastric dysrhythmias were recorded in 66% of the GP and 65% of the FD patients after the water load. Symptoms and GMA were similar in both groups after ingestion of the nutrient bar.ConclusionThe similarity in GMA responses and symptoms after ingestion of solid or liquid test meals suggests GP and FD are closely related gastric neuromuscular disorders.
Genotype of autosomal recessive congenital ichthyosis from a tertiary care center in India
Authors: Chiramel Minu Jose , Mathew Lydia , Athirayath Rekha , Chapla Aaron , Sathishkumar Dharshini , Mani Thenmozhi , Danda Sumita , George Renu 
Published: April 2022

AbstractBackgroundAutosomal recessive congenital ichthyosis (ARCI) refers to non‐syndromic ichthyosis caused by mutations in one of the 13 identified genes. There are limited data on the genotype of ARCI and its phenotypic correlation from India.ObjectivesThe aim of this study was to characterize the genotype of ARCI among patients from the Indian subcontinent.MethodsTwenty‐eight patients clinically diagnosed as ARCI were recruited prospectively from September 2017 to June 2019 (21 months). DNA was extracted from peripheral blood and analyzed for the 13 described ARCI genes—TGM1, ABCA12, ALOX12B, ALOXE3, CERS3, CYP4F22, LIPN, NIPAL4, PNPLA1, SDR9C7, SLC27A4, SULT2B1, and CASP14 by next‐generation sequencing using an in‐house panel. The variants identified were confirmed by Sanger sequencing and compared with known pathogenic variants to establish pathogenicity. We also attempted to correlate the phenotype with the genotype.ResultsAmong the 28 patients recruited (M = 17, F = 11), we identified phenotypes of congenital ichthyosiform erythroderma in 12 (42.9%), 8 with lamellar ichthyosis (28.6%), 5 with intermediate phenotype (17.9%), and 3 with bathing suit ichthyosis (10.7%). Pathogenic and likely pathogenic variants were identified in 22 (78.6%) patients, involving 7 out of the 13 known ARCI genes while 6 (21.4%) did not have pathogenic variants. These included TGM1 mutation in 6 (21.4%), ALOX12B and ALOXE3 in 4 (14.3%) each, NIPAL4 and PNPLA1 in 3 (10.7%) each, and ABCA12 and CERS3 in 1 (3.6%) patient each. Previously unknown pathogenic variants were found in 59.1 % of patients.ConclusionsOur patients with ARCI were found to have genotypes as previously described in other populations.
Against Slagle’s Reading of Eliminative Materialism on Self‐Defeating
Authors: Tümkaya Serdal 
Published: April 2022

AbstractJim Slagle claims that eliminative materialism (EM) denies some of the mind’s self‐evident properties, such as intentionality, qualia and the view that beliefs are real or veridical. I, herein, will argue that what EM denies is actually the folk psychological notion of belief, not belief as such. The Churchlands construe propositional belief as merely one kind of representation in the larger representational scheme. The point here is not to deny belief, but to construe it as one of the, and possibly a very recent, sorts of representations that the brain uses, not the general or fundamental form thereof.
Development of a novel machine learning‐based weighted modeling approach to incorporate Salmonella enterica heterogeneity on a genetic scale in a dose–response modeling framework
Authors: Karanth Shraddha , Pradhan Abani K. 
Published: April 2022

AbstractEstimating microbial dose–response is an important aspect of a food safety risk assessment. In recent years, there has been considerable interest to advance these models with potential incorporation of gene expression data. The aim of this study was to develop a novel machine learning model that considers the weights of expression of Salmonella genes that could be associated with illness, given exposure, in hosts. Here, an elastic net‐based weighted Poisson regression method was proposed to identify Salmonella enterica genes that could be significantly associated with the illness response, irrespective of serovar. The best‐fit elastic net model was obtained by 10‐fold cross‐validation. The best‐fit elastic net model identified 33 gene expression–dose interaction terms that added to the predictability of the model. Of these, nine genes associated with Salmonella metabolism and virulence were found to be significant by the best‐fit Poisson regression model (p < 0.05). This method could improve or redefine dose–response relationships for illness from relative proportions of significant genes from a microbial genetic dataset, which would help in refining endpoint and risk estimations.
Parental play supportiveness and kindergartners’ peer problems: Children's playfulness as a potential mediator
Authors: Fung Wing Kai , Chung Kevin Kien Hoa 
Published: April 2022

AbstractThis study examined the mediating role of children's playfulness in the relationship between parental play supportiveness and children's prospective peer problems in a sample of Hong Kong Chinese kindergarten children. Participants were parents and teachers of 108 local children (56% boys, mean age = 60.0 months). At time 1, parents reported their supportiveness towards household play and their child's playfulness through a questionnaire. Six months later at time 2, teachers reported children's peer problems as exhibited in the kindergarten. A path analytic model revealed that, controlling for child age, gender, and birth order, parental play supportiveness and children's playfulness at time 1 were positively associated, and that playfulness at time 1 negatively predicted peer problems at time 2. The indirect relationship between parental play supportiveness and peer problems as mediated through playfulness was significant, whereas the direct relationship between parental play supportiveness and peer problems was non‐significant. These findings suggest that parents who support children's household play may promote children's capacity to establish positive peer relationships by improving their level of playfulness. Practically, the results highlight the utility of fostering parental play supportiveness and children's playfulness to support kindergarten children's social development.
The basic leucine zipper transcription factor OsbZIP83 and the glutaredoxins OsGRX6 and OsGRX9 facilitate rice iron utilization under the control of OsHRZ ubiquitin ligases
Authors: Kobayashi Takanori , Shinkawa Haruka , Nagano Atsushi J. , Nishizawa Naoko K. 
Published: April 2022

SUMMARYUnder low iron availability, plants induce the expression of various genes for iron uptake and translocation. The rice (Oryza sativa) ubiquitin ligases OsHRZ1 and OsHRZ2 cause overall repression of these iron‐related genes at the transcript level, but their protein‐level regulation is unclear. We conducted a proteome analysis to identify key regulators whose abundance was regulated by OsHRZs at the protein level. In response to iron deficiency or OsHRZ knockdown, many genes showed differential regulation between the transcript and protein levels, including the TGA‐type basic leucine zipper transcription factor OsbZIP83. We also identified two glutaredoxins, OsGRX6 and OsGRX9, as OsHRZ‐interacting proteins in yeast and plant cells. OsGRX6 also interacted with OsbZIP83. Our in vitro degradation assay suggested that OsbZIP83, OsGRX6 and OsGRX9 proteins are subjected to 26S proteasome‐ and OsHRZ‐dependent degradation. Proteome analysis and our in vitro degradation assay also suggested that OsbZIP83 protein was preferentially degraded under iron‐deficient conditions in rice roots. Transgenic rice lines overexpressing OsGRX9 and OsbZIP83 showed improved tolerance to iron deficiency. Expression of iron‐related genes was affected in the OsGRX9 and OsGRX6 knockdown lines, suggesting disturbed iron utilization and signaling. OsbZIP83 overexpression lines showed enhanced expression of OsYSL2 and OsNAS3, which are involved in internal iron translocation, in addition to OsGRX9 and genes related to phytoalexin biosynthesis and the salicylic acid pathway. The results suggest that OsbZIP83, OsGRX6 and OsGRX9 facilitate iron utilization downstream of the OsHRZ pathway.
CD14 recycling modulates LPS‐induced inflammatory responses of murine macrophages
Authors: Ciesielska Anna , Krawczyk Marta , Sas‐Nowosielska Hanna , Hromada‐Judycka Aneta , Kwiatkowska Katarzyna 
Published: April 2022

AbstractTLR4 is activated by the bacterial endotoxin lipopolysaccharide (LPS) and triggers two proinflammatory signaling cascades: a MyD88‐dependent one in the plasma membrane, and the following TRIF‐dependent one in endosomes. An inadequate inflammatory reaction can be detrimental for the organism by leading to sepsis. Therefore, novel approaches to therapeutic modulation of TLR4 signaling are being sought after. The TLR4 activity is tightly connected with the presence of CD14, a GPI‐anchored protein that transfers LPS monomers to the receptor and controls its endocytosis. In this study we focused on CD14 trafficking as a still poorly understood factor affecting TLR4 activity. Two independent assays were used to show that after endocytosis CD14 can recycle back to the plasma membrane in both unstimulated and stimulated cells. This route of CD14 trafficking can be controlled by sorting nexins (SNX) 1, 2 and 6, and is important for maintaining the surface level and the total level of CD14, but can also affect the amount of TLR4. Silencing of these SNXs attenuated especially the CD14‐dependent endosomal signaling of TLR4, making them a new target for therapeutic regulation of the inflammatory response of macrophages to LPS.
Study on the Main Controlling Factors and Formation Mechanism of Motion States of Bottom Hole Assembly in Slightly Deviated Wells
Authors: Li Wei , Wu Baizhi , Huang Genlu , Yu Fan , Ni Hongjian 
Published: April 2022

Abstract
With the further development of deep oil and gas resources, the deep and ultra-deep wells are used widely, which make lateral vibration of bottom hole assembly (BHA) more violent. Lateral vibration greatly restricts the service efficiency and life of downhole devices. To study the main controlling factors and formation mechanism of lateral motion states in slightly deviated wells, the numerical simulation and indoor experiment were conducted. A drillstring dynamics model with three-dimensional beam elements was built, which combined the advantage of the finite element method and the beam-column theory. The simulation and experiment were carried out to analyze the motion states and motion laws under different drilling coefficients. Four kinds of conventional motion states were classified, and the physical characteristics, forming conditions, and identification methods were given. Four main controlling factors of motion states were summarized, and the formation mechanism was discussed through the principle of least energy consumption and least work consumption. The researches show that the numerical and experimental results are in good agreement. Meanwhile, well inclination, rotational speed, and friction coefficient have significant effects on motion states. The states contain forward whirl, random motion, and backward whirl in vertical wells, and circular arc swing in slightly deviated wells. Moreover, radial component of gravity promotes the formation of circular arc swing, centrifugal force promotes forward whirl before drillstring contacts wellbore and promotes backward whirl after that, tangential friction promotes backward whirl, and internal friction promotes forward whirl. Finally, the paper holds that BHA in vertical wells should operate in the motion state with least output power of the system and also operate in the motion state with least input power of external force during steady motion. The study can lay a theoretical foundation for predicting, identifying, and controlling motion states in drilling field to reduce vibration and improve drilling efficiency.