bluuebunny/crossref_metadata_2025_split huggingface dataset:
BRAND EQUITY PRODUK MOTHER OF PEARL DALAM AKUN INSTAGRAM @MOP.BEAUTY
Authors: Ratnasari Eny , Megasari Puspita , Sufa Siska Armawati 
Published: October 2022

Mother of Pearl adalah brand base makeup baru yang dimiliki oleh Tasya Farasya, seorang beauty influencer di Indonesia. Tujuan penelitian ini adalah untuk mengetahui latar belakang upaya branding yang dilakukan oleh produk Mother of Pearl sebagai make up based product di Indonesia. Selain itu, penelitian ini ingin menguraikan upaya branding khususnya brand equity yang dilakukan oleh Mother of Pearl. Tasya Farasya sebagai seorang social media beauty influencer memiliki pengaruh dalam upaya branding yang dilakukan oleh MOP. Penelitian ini menggunakan metode kualitatif. Pengumpulan data dilakukan dengan cara melakukan wawancara, observasi dan studi pustaka. Upaya branding yang dilakukan oleh Mother of Pearl (MOP) salah satunya adalah melalui media sosial Instagram MOP yakni @mop.beauty dengan mengunggah berbagai konten dalam bentuk photo post, carousel, story, reels, maupun video. Tim konten MOP berusaha untuk selalu melakukan branding MOP sebagai luxury and exclusive brand dari desain yang diperlihatkan dalam akun Instagram MOP. Upaya branding khususnya brand equity yang dilakukan oleh Mother of Pearl adalah sebagai berikut: (1) Mother of Pearl Berasal dari Nama Asli Tasya Farasya; (2) Menggunakan Hashtag #MotherKnowsBest sebagai Tagline; (3) Membangun Ciri Khas Produk sebagai Flawless Makeup Products; (4) Value yang Diusung Brand Mother of Pearl Sebagai Diferensiasi Brand; (5) Warna Ungu sebagai Ciri Khas Brand Mother of Pearl agar Terkesan Luxury Brand; (6) Pesan yang Disampaikan dalam Akun Instagram MOP Terkesan untuk Audiens yang Tinggal di Kota Besar; (7) Membangun Kedekatan dengan Pengikut Melalui Sayembara; (8) Penegasan sebagai Exclusive Brand yang hanya Dijual di Marketplace Sociolla.
Diagnostic value of bronchoalveolar lavage fluid metagenomic next-generation sequencing in pediatric pneumonia
Authors: Deng Wenhua , Xu Huan , Wu Yabin , Li Jie 
Published: October 2022

ObjectivesThe aim of this study was to evaluate the diagnostic value of bronchoalveolar lavage fluid (BALF) metagenomic next-generation sequencing (mNGS) versus conventional microbiological tests (CMTs) for pediatric pneumonia.MethodsThis retrospective observational study enrolled 103 children who were diagnosed with pneumonia and hospitalized at Hubei Maternity and Child Health Care Hospital between 15 October 2020 and 15 February 2022. The pneumonia diagnosis was based on clinical manifestations, lung imaging, and microbiological tests. Pathogens in the lower respiratory tract were detected using CMTs and BALF mNGS (of DNA and RNA). The diagnostic performance of BALF mNGS was compared with that of CMTs.ResultsIn 96 patients, pathogens were identified by microbiological tests. The overall pathogen detection rate of mNGS was significantly higher than that of CMTs (91.3% vs. 59.2%, p = 0.000). The diagnostic performance of mNGS varied for different pathogens; however, its sensitivity and accuracy for diagnosing bacterial and viral infections were both higher than those of CMTs (p = 0.000). For the diagnosis of fungi, the sensitivity of mNGS (87.5%) was higher than that of CMTs (25%); however, its specificity and accuracy were lower than those of CMTs (p &lt; 0.01). For the diagnosis of Mycoplasma pneumoniae, the specificity (98.8%) and accuracy (88.3%) of mNGS were high; however, its sensitivity (42.1%) was significantly lower than that of CMTs (100%) (p = 0.001). In 96 patients with definite pathogens, 52 cases (50.5%) were infected with a single pathogen, while 44 cases (42.7%) had polymicrobial infections. Virus–bacteria and virus–virus co-infections were the most common. Staphylococcus aureus, Haemophilus influenzae, rhinovirus, cytomegalovirus, parainfluenza virus, and fungi were more likely to be associated with polymicrobial infections.ConclusionsBALF mNGS improved the detection rate of pediatric pneumonia, especially in mixed infections. The diagnostic performance of BALF mNGS varies according to pathogen type. mNGS can be used to supplement CMTs. A combination of mNGS and CMTs may be the best diagnostic strategy.
Detection of IMP-4 and SFO-1 co-producing ST51 Enterobacter hormaechei clinical isolates
Authors: Qiao Jie , Ge Haoyu , Xu Hao , Guo Xiaobing , Liu Ruishan , Li Chenyu , Chen Ruyan , Zheng Beiwen , Gou Jianjun 
Published: October 2022

PurposeTo explore the genetic characteristics of the IMP-4 and SFO-1 co-producing multidrug-resistant (MDR) clinical isolates, Enterobacter hormaechei YQ13422hy and YQ13530hy.MethodsMALDI-TOF MS was used for species identification. Antibiotic resistance genes (ARGs) were tested by PCR and Sanger sequencing analysis. In addition to agar dilution, broth microdilution was used for antimicrobial susceptibility testing (AST). Whole-genome sequencing (WGS) analysis was conducted using the Illumina NovaSeq 6000 and Oxford Nanopore platforms. Annotation was performed by RAST on the genome. The phylogenetic tree was achieved using kSNP3.0. Plasmid characterization was conducted using S1-pulsed-field gel electrophoresis (S1-PFGE), Southern blotting, conjugation experiments, and whole genome sequencing (WGS). An in-depth study of the conjugation module was conducted using the OriTFinder website. The genetic context of blaIMP-4 and blaSFO-1 was analyzed using BLAST Ring Image Generator (BRIG) and Easyfig 2.3.ResultsYQ13422hy and YQ13530hy, two MDR strains of ST51 E. hormaechei harboring blaIMP-4 and blaSFO-1, were identified. They were only sensitive to meropenem, amikacin and polymyxin B, and were resistant to cephalosporins, aztreonam, piperacillin/tazobactam and aminoglycosides, intermediate to imipenem. The genetic context surrounding blaIMP-4 was 5′CS-hin-1-IS26-IntI1-blaIMP-4-IS6100-ecoRII. The integron of blaIMP-4 is In823, which is the array of gene cassettes of 5′CS-blaIMP-4. Phylogenetic analysis demonstrated that E. hormaechei YQ13422hy and YQ13530hy belonged to the same small clusters with a high degree of homology.ConclusionThis observation revealed the dissemination of the blaIMP-4 gene in E. hormaechei in China. We found that blaIMP-4 and blaSFO-1 co-exist in MDR clinical E. hormaechei isolates. This work showed a transferable IncN-type plasmid carrying the blaIMP-4 resistance gene in E. hormaechei. We examined the potential resistance mechanisms of pYQ13422-IMP-4 and pYQ13422-SFO-1, along with their detailed genetic contexts.
Diabetes associated with cervical carcinoma among high-risk HPV-infected patients with cytologically diagnosed high grade squamous intraepithelial lesion
Authors: Yue Chaoyan , Zhang Chunyi , Ying Chunmei , Jiang Hua 
Published: October 2022

BackgroundDiabetes causes metabolic disorders and immune changes that may be potential triggers of cervical cancer. Therefore, diabetes is not a “bystander” to cervical cancer. However, the conclusion that diabetes promotes cervical cancer lacks clinical epidemiological evidence, and the reported potential association between diabetes and cervical cancer is controversial.MethodsWe conducted an explorative cross-sectional study of 791 women with cytological HGSIL and HR-HPV, who attended the cervical clinic of the largest academic women’s hospital in China from May 2019 to March 2022. After cervical screening, patients who were requiring colposcopy were tested for HbA1c. HbA1c level of 6.5% or higher defines diabetes and HbA1c level of 5.7%-6.4% was defined as prediabetes. The relationship between diabetes and cervical cancer was observed by a dose-response graph. Subgroup analysis and multivariate logistic regression analysis were conducted to estimate the associations between diabetes and cervical cancer.ResultsAmong HGSIL patients with high-risk HPV infection, compared with women with HbA1c &lt;5.7%, the odds ratio for women with prediabetes was 1.72 (95% CI: 0.87-3.41) and the odds ratio for women with diabetes was 3.29 (95% CI: 1.10-9.80) for cervical cancer. Sensitivity analysis showed that diabetes was significantly associated with cervical cancer in different age groups and different HPV variant. E-value analysis showed robustness to unmeasured confounding.ConclusionsIn patients with HR-HPV combined with HGSIL, diabetes and prediabetes are associated with cervical cancer.
Baricitinib is potentially effective in the treatment of refractory livedoid vasculopathy
Authors: Han Yuyang , Tu Ping 
Published: October 2022

BackgroundLivedoid vasculopathy is a rare, chronic, and recurrent disease with limited effective treatments. Its etiopathogenesis remains incompletely understood. Baricitinib, a selective Janus kinase 1 and 2 inhibitor, has been used to treat rheumatoid arthritis and could reduce the disease severity in patients with livedoid vasculopathy.MethodsWe retrospectively observed eight patients who received 2 mg/day of baricitinib for the treatment of refractory livedoid vasculopathy. We evaluated their clinical scores before and after treatment to determine its effectiveness and safety.ResultsImprovement in livedoid vasculopathy was observed with significant regression in the clinical scores after baricitinib treatment. The mean clinical scores were 7.0 ± 1.6 and 1.4 ± 1.2 before and after baricitinib treatment, respectively (P &lt;0.01). Furthermore, six out of the eight patients achieved a clinical score of 0 or 2 after treatment. These scores indicated remission. Clinical findings, including erythema, ulceration, and pain, improved in all the patients. The remission times ranged from 3 to 13 weeks, with a mean remission time of 7.75 ± 3.45 weeks. There were no reports of adverse events in any patient.ConclusionsOur study showed that baricitinib treatment was safe and could significantly relieve the signs and symptoms of livedoid vasculopathy. However, randomized controlled studies should be conducted to confirm these results.
Monocyte response after colorectal surgery: A prospective cohort study
Authors: Edomskis Pim P. , Dik Willem A. , Sparreboom Cloë L. , Nagtzaam Nicole M. A. , van Oudenaren Adrie , Lambrichts Daniël P. V. , Bayon Yves , van Dongen Noah N. N. , Menon Anand G. , de Graaf Eelco J. R. , Coene Peter Paul L. O. , Lange Johan F. , Leenen Pieter J. M. 
Published: October 2022

BackgroundTumor resection is the common approach in patients with colorectal malignancy. Profound insight into inflammatory changes that accompany the normal post-operative stress response will establish reference parameters useful for identification of putative complications. Alterations in circulating monocytes might be indicative as these cells are considered to be the most responsive leukocytes to trauma. Therefore, the aim of this study is to assess the monocyte subset kinetic and phenotypic changes in response to surgery.MethodsFifty patients undergoing colorectal tumor resection were included in a multicenter prospective cohort study. Blood samples were collected early in the morning prior to surgery and the next days through postoperative day three for flowcytometric analysis. Leukocyte subtypes were identified and expression of activation stage-related markers by monocyte subsets was quantified.ResultsChanges in leukocyte subset composition and monocyte subset phenotypes were most prominent at the first day postoperatively, after which these parameters typically returned to normal or near-normal preoperative values. The immunophenotypic alterations after surgery were most notable in classical and intermediate monocytes. These included up-regulation of activation markers CD64 and CD62L, but down-regulation of HLA-DR and CD54. Markers of de-activation, CD163 and CD206, were consistently increasingly expressed.Discussion/conclusionThe current study gives detailed insight into the peripheral blood leukocyte response after colorectal cancer surgery. This form of short-term stress induces a rapid and significant redistribution of immune cells. Immunophenotypic alterations in monocytes as a response to surgery suggest a mixed profile of cellular activation and de-activation.
Low-dose immunogenic chemotherapeutics promotes immune checkpoint blockade in microsatellite stability colon cancer
Authors: Fang Yuhang , Sun Haoyu , Xiao Xinghui , Tang Maoxing , Tian Zhigang , Wei Haiming , Sun Rui , Zheng Xiaodong 
Published: October 2022

More than 85% of colorectal cancer (CRC) patients, who are with microsatellite stability (MSS), are resistant to immune checkpoint blockade (ICB) treatment. To overcome this resistance, combination therapy with chemotherapy is the most common choice. However, many CRC patients do not benefit more from combination therapy than chemotherapy alone. We hypothesize that severe immunosuppression, caused by chemotherapy administered at the maximum tolerated dose, antagonizes the ICB treatment. In this study, we found that low-dose oxaliplatin (OX), an immunogenic cell death (ICD)-induced drug, increased the antitumor response of TIGIT blockade against CT26 tumor, which is regarded as a MSS tumor. Combined treatment with OX and TIGIT blockade fostered CD8+ T-cell infiltration into tumors and delayed tumor progression. Importantly, only low-dose immunogenic chemotherapeutics successfully sensitized CT26 tumors to TIGIT blockade. In contrast, full-dose OX induces severe immunosuppression and impaired the efficacy of combination therapy. Further, we also found that lack of synergy between nonimmunogenic chemotherapeutics and TIGIT blockade. Consequently, this study suggests that the strategies of combination treatment of chemotherapy and ICB should be re-evaluated. The chemotherapeutics should be chosen for the potential to ICD and the dosage and regimen should be also optimized.
Case report: Patient specific combination of surgery and immunotherapy in advanced squamous cell carcinoma of the head and neck – a case series and review of literature
Authors: Olmos Manuel , Lutz Rainer , Büntemeyer Tjark-Ole , Glajzer Jacek , Nobis Christopher-Philipp , Ries Jutta , Möst Tobias , Eckstein Markus , Hecht Markus , Gostian Antoniu-Oreste , Erdmann Michael , Foerster Yannick , Kesting Marco , Weber Manuel 
Published: October 2022

BackgroundPrognosis of patients with recurrent or metastatic head and neck cancer is generally poor. Adjuvant immunotherapy (IT) featuring immune checkpoint inhibition (ICI) is standard of care in advanced stage head and neck squamous cell carcinoma (HNSCC) and cutaneous squamous cell carcinoma (CSCC). ICI response rates in CSCC are described as higher than in HNSCC. IT is constantly shifting into earlier disease stages which confronts the surgeon with immunotherapeutically pre-treated patients. It is therefore becoming increasingly difficult to assess which patients with symptomatic tumor disease and a lack of curative surgical option might benefit from salvage surgery.Case presentationsThe following 6 cases describe therapeutic decision-making regarding ICI and (salvage) surgery in patients with advanced stage HNSCC or CSCC. Cases A and B focus on neoadjuvant ICI followed by salvage surgery. In Cases C and D salvage surgery was performed after short-term stabilization with partial response to ICI. The last two cases (Cases E and F) address the surgical approach after failure of ICI. All cases are discussed in the context of the current study landscape and with focus on individual decision-making. For better understanding, a timetable of the clinical course is given for each case.ConclusionsICI is rapidly expanding its frontiers into the neoadjuvant setting, frequently confronting the surgeon with heavily pretreated patients. Salvage surgery is a viable therapeutic concept despite the rise of systemic treatment options. Decision-making on surgical intervention in case of a salvage surgery remains an individual choice. For neoadjuvant ICI monitoring regarding pathological tumor response or tumor necrosis rate, we suggest correlation between the initial biopsy and the definite tumor resectate in order to increase its significance as a surrogate marker. Scheduling of neoadjuvant ICI should be further investigated, as recent studies indicate better outcomes with shorter time frames.
Thresholds of hypoxia of two Red Sea coral species (Porites sp. and Galaxea fascicularis)
Authors: Alva García Jacqueline V. , Klein Shannon G. , Alamoudi Taiba , Arossa Silvia , Parry Anieka J. , Steckbauer Alexandra , Duarte Carlos M. 
Published: October 2022

Anthropogenic pressures have driven large-scale declines in coral cover on &gt;50% of tropical coral reefs. Most research efforts have focused on ocean warming, ocean acidification, and overfishing impacts. Despite increasing instances of reef-associated hypoxic events, the role of reduced O2 in affecting coral reef performance is largely unknown. Here, we assessed the hypoxic thresholds of two Red Sea coral species: Porites sp. and Galaxea fascicularis. We exposed coral fragments of both species to one control treatment (6.8 mg O2 L−1) and three reduced dissolved oxygen treatments (5.25, 3.5, and 1.25 mg O2 L−1) during a 10-day experiment. Across the two species, maximum (Fv/Fm) and effective (F′/Fm′) photochemical efficiency, chlorophyll a, and dark respiration declined under the lowest O2 treatment (1.25 mg O2 L−1). Porites sp. coral fragments, however, were remarkably resistant and showed no signs of sublethal bleaching after 10 days of exposure to reduced O2. Conversely, 17% of G. fascicularis fragments bleached after only three nights of exposure to the lowest O2 treatment (1.25 mg O2 L−1). Our data show that longer-term hypoxic events (i.e., days to weeks) can induce coral bleaching, but these effects depend on the extent of O2 reduction and are likely species-specific. Importantly, the levels of O2 usually defined as hypoxic (~2.0 to 2.8 mg O2 L−1) do not adequately capture the thresholds reported here. Hence, further research is urgently needed to more accurately describe the vulnerability of coral taxa to hypoxic and anoxic events.
Inflammation-related genes and immune infiltration landscape identified in kainite-induced temporal lobe epilepsy based on integrated bioinformatics analysis
Authors: Wang Li , Duan Chunmei , Wang Ruodan , Chen Lifa , Wang Yue 
Published: October 2022

BackgroundTemporal lobe epilepsy (TLE) is a common brain disease. However, the pathogenesis of TLE and its relationship with immune infiltration remains unclear. We attempted to identify inflammation-related genes (IRGs) and the immune cell infiltration pattern involved in the pathological process of TLE via bioinformatics analysis.Materials and methodsThe GSE88992 dataset was downloaded from the Gene Expression Omnibus (GEO) database to perform differentially expressed genes screening and weighted gene co-expression network analysis (WGCNA). Subsequently, the functional enrichment analysis was performed to explore the biological function of the differentially expressed IRGs (DEIRGs). The hub genes were further identified by the CytoHubba algorithm and validated by an external dataset (GSE60772). Furthermore, the CIBERSORT algorithm was applied to assess the differential immune cell infiltration between control and TLE groups. Finally, we used the DGIbd database to screen the candidate drugs for TLE.Results34 DEIRGs (33 up-regulated and 1 down-regulated gene) were identified, and they were significantly enriched in inflammation- and immune-related pathways. Subsequently, 4 hub DEIRGs (Ptgs2, Jun, Icam1, Il6) were further identified. Immune cell infiltration analysis revealed that T cells CD4 memory resting, NK cells activated, Monocytes and Dendritic cells activated were involved in the TLE development. Besides, there was a significant correlation between hub DEIRGs and some of the specific immune cells.Conclusion4 hub DEIRGs (Ptgs2, Jun, Icam1, Il6) were associated with the pathogenesis of TLE via regulation of immune cell functions, which provided a novel perspective for the understanding of TLE.
Case report: Significant response to PD-L1 inhibitor after resistance to PD-1 inhibitor in an advanced alpha-fetoprotein-positive gastric cancer
Authors: Wang Liyu , Feng Ying , Huang Anquan , Shi Jianming , Zhang Qinying , Zhu Fan , Lv Bin , Guo Fen , Zou Tianming , Zhang Luyao 
Published: October 2022

Alpha-fetoprotein-positive gastric cancer (AFPGC) is a type of gastric cancer with a high degree of malignancy. The disease is more common in the elderly, with a high prevalence in males and generally atypical clinical manifestations. For advanced patients, the current treatment options are limited and, to date, few cases of advanced AFPGC have been treated successfully with conventional chemotherapy. With the development of molecular biology and immunology, tumor immunotherapy offers more therapeutic options to patients with advanced gastric cancer. This study describes a case of advanced gastric cancer in a young woman with a high blood alpha-fetoprotein (AFP) level (&gt;54,000 ng/mL). The patient showed initial promising results when programmed cell death-1 (PD-1) inhibitor treatment was combined with chemotherapy after systemic chemotherapy failed. When the disease progressed again after 129 days, adjustment of the treatment regimen to Atezolizumab in combination with Irinotecan and Surufatinib capsules achieved partial remission (PR). There were no immune-related pneumonia, myocarditis, or other adverse effects observed. The patient currently has an overall survival of more than 14 months. This case demonstrated that switching from PD-1 inhibitor to programmed cell death-Ligand 1 (PD-L1) inhibitor therapy may overcome potential resistance. It providing a reference for immunotherapy of patients with AFP-positive advanced gastric cancer.
Adaptation and validation of two annotation scales for assessing social skills in a corpus of multimodal collaborative interactions
Authors: Hamet Bagnou Jennifer , Prigent Elise , Martin Jean-Claude , Clavel Céline 
Published: October 2022

ContextBehavioral observation scales are important for understanding and assessing social skills. In the context of collaborative problem-solving (CPS) skills, considered essential in the 21st century, there are no validated scales in French that can be adapted to different CPS tasks. The aim of this study is to adapt and validate, by annotating a new video corpus of dyadic interactions that we have collected, two observational scales allowing us to qualitatively assess CPS skills: the Social Performance Rating Scale (SPRS) and the Social Skills of Collaboration Scale (SSC).MethodThe construct validity of these two scales was assessed by exploratory factor analysis and inter-item correlations. We also checked inter-judge agreement using inter-class correlation coefficients. Internal consistency was determined using Cronbach’s alpha and convergent and divergent validity by assessing correlations between the two scales and measures of depression and alexithymia. Finally, the discriminative properties of the two scales were analyzed by comparing the scores obtained by a group of anxious individuals and a non-anxious control group.ResultsThe results show that our two scales have excellent inter-item correlations. Internal consistency is excellent (alpha SPRS =0.90; SSC = 0.93). Inter-rater agreement ranged from moderate to high. Finally, convergent validity was significant with the alexithymia scale, as was divergent validity with the depression scale. Anxious individuals had lower scores on both scales than non-anxious individuals.ConclusionBoth scales show good psychometric properties for assessing social skills relevant to different collaborative tasks. They also identify individuals with difficulties in social interaction. Thus, they could allow monitoring the effectiveness of training social skills useful in CPS.