bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of heterospecific pollen on stigma behavior in Campsis radicans: Causes and consequences
Authors: Zou Ting‐Ting , Wang Chun‐Hui , Lyu Sen‐Tao , Yu Xiaolei , Deng Lu‐Xi , Liu Wei‐Qi , Dai Jie , Wang Xiao‐Fan 
Published: May 2022

AbstractPremisePollinator sharing of co‐flowering plants may result in interspecific pollen receipt with a fitness cost. However, the underlying factors that determine the effects of heterospecific pollen (HP) are not fully understood. Moreover, the cost of stigma closure induced by HP may be more severe for plants with special touch‐sensitive stigmas than for plants with non‐touch‐sensitive stigmas. Very few studies have assessed HP effects on stigma behavior.MethodsWe conducted hand‐pollination experiments with 10 HP donors to estimate HP effects on stigma behavior and stigmatic pollen germination in Campsis radicans (Bignoniaceae) at low and high pollen loads. We assessed the role of phylogenetic distance between donor and recipient, pollen size, and pollen aperture number in mediating HP effects. Additionally, we observed pollen tube growth to determine the conspecific pollen‐tube‐growth advantage.ResultsStigma behavior differed significantly with HP of different species. Pollen load increased, while pollen size decreased, the percentage of permanent closure and stigmatic germination of HP. Stigmatic HP germination increased with increasing aperture number. However, HP effects did not depend on phylogenetic distance. In addition, conspecific pollen had a pollen‐tube‐growth advantage over HP.ConclusionsOur results provide a good basis for understanding the stigma‐pollen recognition process of plant taxa with touch‐sensitive stigmas. We concluded that certain flowering traits drive the HP effects on the post‐pollination period. To better understand the impact of pollinator sharing and interspecific pollen transfer on plant evolution, we highlight the importance of evaluating more factors that determine HP effects at the community level.
TP53‐altered chronic lymphocytic leukemia treated with firstline Bruton's tyrosine kinase inhibitor‐based therapy: A retrospective analysis
Authors: Cherng Hua‐Jay J. , Khwaja Raamis , Kanagal‐Shamanna Rashmi , Tang Guilin , Burger Jan , Thompson Philip , Ferrajoli Alessandra , Estrov Zeev , Sasaki Koji , Sampath Deepa , Wang Xuemei , Kantarjian Hagop , Keating Michael , Wierda William G. , Jain Nitin 
Published: May 2022

AbstractLong‐term follow up of prospective studies has shown that continuous Bruton's tyrosine kinase inhibitor (BTKi) therapy leads to durable remissions in previously untreated patients with TP53‐altered chronic lymphocytic leukemia (CLL); however, it is unknown how variant allele frequency (VAF) of TP53 mutation (TP53‐m) or percentage of cells with deletion of chromosome 17p [del(17p)] influences efficacy of firstline BTKi. We performed a retrospective analysis of 130 patients with CLL with baseline del(17p) and/or TP53‐m treated with BTKi with or without the BCL2 inhibitor venetoclax (VEN) and with or without CD20 antibody in the firstline setting. A total of 104/130 (80%) patients had del(17p). TP53‐m was noted in 89/110 (81%) patients tested; there were 101 unique TP53‐m with an available VAF. The 4‐year progression‐free survival (PFS) and overall survival (OS) rates were 72.9% and 83.6%. No baseline characteristics including IGHV mutation status and number of TP53 alterations were associated with significant differences in PFS or OS, though a trend toward shorter PFS with increasing karyotypic complexity (hazard ratio 1.08, p = .066) was observed. Del(17p) was identified in <25% of cells in 26/104 (25%) of patients, and 28/101 (28%) of TP53‐m were low‐burden with a VAF of <10%; outcomes of these patients were similar to those with high‐burden lesions. This study suggests that low‐burden TP53 alterations should not be ignored when assessing genomic risk in CLL in the era of targeted therapy.
Expanding ACTA2 genotypes with corresponding phenotypes overlapping with smooth muscle dysfunction syndrome
Authors: Kaw Anita , Kaw Kaveeta , Hostetler Ellen M. , Beleza‐Meireles Ana , Smith‐Collins Adam , Armstrong Catherine , Scurr Ingrid , Cotts Timothy , Aatre Rajani , Bamshad Michael J. , Earl Dawn , Groner Abraham , Agre Katherine , Raveh Yehuda , Kwartler Callie S. , Milewicz Dianna M. 
Published: May 2022

AbstractPathogenic variants in ACTA2, encoding smooth muscle α‐actin, predispose to thoracic aortic aneurysms and dissections. ACTA2 variants altering arginine 179 predispose to a more severe, multisystemic disease termed smooth muscle dysfunction syndrome (SMDS; OMIM 613834). Vascular complications of SMDS include patent ductus arteriosus (PDA) or aortopulmonary window, early‐onset thoracic aortic disease (TAD), moyamoya‐like cerebrovascular disease, and primary pulmonary hypertension. Patients also have dysfunction of other smooth muscle‐dependent systems, including congenital mydriasis, hypotonic bladder, and gut hypoperistalsis. Here, we describe five patients with novel heterozygous ACTA2 missense variants, p.Arg179Gly, p.Met46Arg, p.Thr204Ile, p.Arg39Cys, and p.Ile66Asn, who have clinical complications that align or overlap with SMDS. Patients with the ACTA2 p.Arg179Gly and p.Thr204Ile variants display classic features of SMDS. The patient with the ACTA2 p.Met46Arg variant exhibits exclusively vascular complications of SMDS, including early‐onset TAD, PDA, and moyamoya‐like cerebrovascular disease. The patient with the ACTA2 p.Ile66Asn variant has an unusual vascular complication, a large fusiform internal carotid artery aneurysm. The patient with the ACTA2 p.Arg39Cys variant has pulmonary, gastrointestinal, and genitourinary complications of SMDS but no vascular manifestations. Identifying pathogenic ACTA2 variants associated with features of SMDS is critical for aggressive surveillance and management of vascular and nonvascular complications and delineating the molecular pathogenesis of SMDS.
Further phenotypic delineation of Alazami syndrome
Authors: Al‐Hinai Abdulhamid , Al‐Hashmi Samiya , Ganesh Anuradha , Al‐Hashmi Nadia , Al‐Saegh Abeer , Al‐Mamari Watfa , Al‐Murshedi Fathiya , Al‐Thihli Khalid , Al‐Kindi Adila , Al‐Maawali Almundher 
Published: May 2022

AbstractAlazami syndrome (AS) is an autosomal recessive condition characterized by the cardinal features of severe growth restriction, moderate to severe intellectual disability, and distinctive facial features. Biallelic pathogenic variants of the LARP7, encoding a chaperone of 7SK noncoding RNA, is implicated in this disease. There are <35 reported cases in the literature. All reported cases share the same three cardinal features of the syndrome. Herein, we report on 12 patients with a confirmed diagnosis of AS from eight unrelated families. The cohort shares the same key feature of the syndrome. Moreover, we report additional phenotypic features, including genito‐renal anomalies, ophthalmological abnormalities, and congenital heart disease. Whole‐exome sequencing was used in all reported cases, implicating a clinical under‐recognition of the syndrome. This report further expands the clinical and molecular characteristics of Alazami syndrome.
Polymer Nanoparticles with Uniform Monomer Sequences for Sequence‐Specific Peptide Recognition
Authors: Saito Yusuke , Honda Ryutaro , Akashi Sotaro , Takimoto Hinata , Nagao Masanori , Miura Yoshiko , Hoshino Yu 
Published: May 2022

AbstractSynthetic polymer nanoparticles (NPs) that recognize and neutralize target biomacromolecules are of considerable interest as “plastic antibodies”, synthetic mimics of antibodies. However, monomer sequences in the synthetic NPs are heterogeneous. The heterogeneity limits the target specificity and safety of the NPs. Herein, we report the synthesis of NPs with uniform monomer sequences for recognition and neutralization of target peptides. A multifunctional oligomer with a precise monomer sequence that recognizes the target peptide was prepared via cycles of reversible addition–fragmentation chain transfer (RAFT) polymerization and flash chromatography. The oligomer or blend of oligomers was used as a chain transfer agent and introduced into poly(N‐isopropyl acrylamide) hydrogel NPs by radical polymerization. Evaluation of the interaction with the peptides revealed that multiple oligomers in NPs cooperatively recognized the sequence of the target peptide and neutralized its toxicity. Effect of sequence, combination, density and molecular weight distribution of precision oligomers on the affinity to the peptides was also investigated.
Studies on the processing of HTPB‐based fast‐burning propellant with trimodal oxidiser distribution and its rheological behaviour
Authors: Chauhan Deepak , Khan Lubna , Mandal Subir Kumar , Jauhari Ashish , Bhattacharyya Sibes Chandra 
Published: May 2022

AbstractComposite propellants based on hydroxyl terminated polybutadiene/ aluminium powder/ammonium perchlorate (HTPB/Al/AP) are commonly used in solid rocket motors (SRM) in defence and space applications. The solid propellant is a polymeric matrix with high solid loadings of metallic fuel and oxidiser along with some additives. Due to high solid loading, the propellant slurry becomes highly viscous and thus becomes difficult to process. In the case of trimodal distribution of the oxidiser, the effect of viscosity becomes more pronounced and it is difficult to mix and cast the propellant under vacuum owing to its high viscosity. In this paper, rheology of the propellant slurry containing oxidiser in trimodal distribution has been studied and an attempt has been made to find the effect of mixing parameters such as oxidiser addition sequence, mixing temperature and plasticiser concentration on the propellant properties and in improving the ease of processing. The study shows that the processability and pot life can be improved by optimising mixing parameters.
Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ13C and δ15N isotopic values
Authors: de Carvalho Camila Carvalho , Marmontel Miriam , Botta Silvina , Emin‐Lima Renata , Costa Alexandra Fernandes , Crema Luciana Carvalho , da Silva Vera Maria Ferreira , Piedade Maria Teresa Fernandez , Secchi Eduardo Resende 
Published: May 2022

Abstract

Threats to aquatic mammals may occur in isolation or in combination, leading to cumulative effects on populations and their ecosystems. Thus, conservation actions must consider the complexity of factors involved in patterns of habitat use, including feeding ecology, such as ontogenetic, spatial and temporal dietary shifts.
The Amazonian manatee (Trichechus inunguis) occurs in a variety of environments, such as várzeas and igapós (flooded areas) and estuaries. Different environments can lead to variation in the diet of manatees according to the availability and abundance of plant species.
Stable isotope analysis (δ13C and δ15N) of manatee teeth was performed to assess spatio‐temporal, ontogenetic and sex‐related patterns in resource use. Mixing models were applied to identify the most consumed plant groups.
In várzeas, there were significant interactions between sex and ontogenetic class and ontogenetic class and time series for δ13C values. For manatees sampled from 1994 to 2004, males and adults showed the highest δ15N values, and aquatic C3, followed by C4 plants, were the groups that most contributed to the species’ diet.
Comparisons among the environments showed the highest δ13C values for manatees from várzeas and the lowest values for manatees from igapós. Várzeas had the highest abundance of C4 plants (high δ13C values), whereas they are scarce in igapós, leading manatees to consume mainly C3 species.
The isotopic values of manatees mirrored those of the most available food sources in each environment, supporting the generalist and opportunist behaviour described for the species. Therefore, threats to the diversity, distribution and abundance of aquatic macrophytes, such as contamination, cattle raising and changes in the hydrological cycle (as a consequence of dams and climate change), will affect the diet of the manatee and should be taken into account when designing conservation strategies for the species.
Revealing the role of the benzyloxy pharmacophore in the design of a new class of monoamine oxidase‐B inhibitors
Authors: Sudevan Sachithra T. , Rangarajan T. M. , Al‐Sehemi Abdullah G. , Nair Aathira S. , Koyiparambath Vishal P. , Mathew Bijo 
Published: May 2022

AbstractThe conceptual layout of monoamine oxidase (MAO) inhibitors has been modified to explore their potential biological application in the case of neurological disorders for the time being. The current review article is an effort to display the summation of innovative conceptual prospects of MAO inhibitors and their intriguing chemistry and bioactivity. Based on this scenario, we emphasize the pivotal role of the benzyloxy moiety attached to scaffolds like oxadiazolones, indolalkylamines, safinamide, caffeine, benzofurans, α‐tetralones, β‐nitrostyrene, benzoquinones, coumarins, indoles, chromones, and chromanone analogs, while acting as an MAO inhibitor.
Syllabic quantity patterns as rhythmic features for Latin authorship attribution
Authors: Corbara Silvia , Moreo Alejandro , Sebastiani Fabrizio 
Published: May 2022

AbstractIt is well known that, within the Latin production of written text, peculiar metric schemes were followed not only in poetic compositions, but also in many prose works. Such metric patterns were based on so‐called syllabic quantity, that is, on the length of the involved syllables, and there is substantial evidence suggesting that certain authors had a preference for certain metric patterns over others. In this research we investigate the possibility to employ syllabic quantity as a base for deriving rhythmic features for the task of computational authorship attribution of Latin prose texts. We test the impact of these features on the authorship attribution task when combined with other topic‐agnostic features. Our experiments, carried out on three different datasets using support vector machines (SVMs) show that rhythmic features based on syllabic quantity are beneficial in discriminating among Latin prose authors.
Impact of Degree of Graphitization, Surface Properties and Particle Size Distribution on Electrochemical Performance of Carbon Anodes for Potassium‐Ion Batteries
Authors: Wrogemann Jens M. , Fromm Olga , Deckwirth Fabian , Beltrop Kolja , Heckmann Andreas , Winter Martin , Placke Tobias 
Published: May 2022

AbstractInvited for this month's cover picture is the group of Dr. Tobias Placke and Prof. Dr. Martin Winter from the MEET Battery Research Center (University of Münster, Münster, Germany). The cover picture shows two characters representing a potassium‐ and lithium‐ion in front of a variety of carbons. The impact of several material properties of carbonaceous materials on potassium‐ion storage is systematically investigated and compared to lithium‐ion chemistry. Read the full text of the Research Article at 10.1002/batt.202200045.
Prüfung von UHFB nach DIN EN 14651 – Zum Einfluss der Probekörperherstellung auf die Prüfergebnisse
Authors: Leutbecher Torsten , Heck Lennart , Schleiting Maximilian 
Published: May 2022

AbstractUltrahochfester Beton (UHFB) wird in der Praxis meist fließfähig bis sehr fließfähig verarbeitet. Der Entwurf der DAfStb‐Richtlinie „Ultrahochfester Beton“ sieht für diesen Fall vor, dass die Balken, an denen die residuelle Biegezugfestigkeit des stahlfaserbewehrten UHFB bestimmt wird, durch Befüllen der Schalung von einer Stirnseite aus hergestellt werden. Um sicherzustellen, dass mit diesem Verfahren reproduzierbare Prüfergebnisse erzielt werden, wurden im Rahmen eines vom Deutschen Ausschuss für Stahlbeton geförderten Forschungsvorhabens 3‐Punkt‐Biegeversuche an Balken aus Feinkorn‐ und Grobkorn‐UHFB durchgeführt und die Verteilung und Orientierung der Fasern ausgewertet. Im Zuge dieser Untersuchungen wurde auch ein alternatives Verfahren der Probekörperherstellung getestet. Für alle Probekörper wurden die residuellen Biegezugfestigkeiten sowie die Faseranzahl, der „effektive“ Fasergehalt und die Faserorientierung nahe dem Bruchquerschnitt bestimmt. Unterschiede zeigten sich in erster Linie in Hinblick auf den Größtkorndurchmesser, während ein signifikanter Einfluss der Herstellmethode nicht zu erkennen war. Die Ergebnisse legen nahe, dass die untersuchten Methoden gleichermaßen für die Probekörperherstellung geeignet sind.
Verteilte Dehnungsmessungen von Spannbetonbauteilen mit faseroptischen Sensoren
Authors: Zdanowicz Katarzyna , Bednarski Łukasz , Howiacki Tomasz , Sieńko Rafał 
Published: May 2022

AbstractDer Beitrag widmet sich der Anwendung der Technologie der verteilten Dehnungsmessung auf Spannbetonbauteilen. Die verteilte faseroptische Technologie, die auf Lichtrückstreuung basiert, ermöglicht Dehnungsmessungen mit hoher räumlicher Auflösung über die gesamte Länge der optischen Faser. Ein solcher Ansatz ersetzt tausende herkömmliche punktuelle Dehnmessstreifen, die in Reihe angeordnet werden, durch eine einzige optische Faser. Im Beitrag werden faseroptische Sensoren aus Verbundwerkstoffen und deren Einsatz für Dehnungsmessungen und Untersuchungen der Rissentwicklung dargestellt. Drei praktische Beispiele von vorgespannten Betonbauteilen werden beschrieben – Lkw‐Waagenplatten, Spannbetonträger mit einer Länge von 24 Metern und eine Brücke mit dem höchsten Pylon in Polen. Die Bauteile wurden in verschiedenen Bauphasen analysiert, während der Herstellung und Erhärtung, der Aktivierung der Spannglieder, des Einbaus und der Probebelastung. Auch als nachträglich installiertes Messsystem kommen die Sensoren zum Einsatz. Ziel dieses Beitrags ist es, die Möglichkeiten von Messungen mit faseroptischen Sensoren aus Verbundwerkstoffen zu schildern.
A seasonal simulation approach for culture depth influence on the temperature for different characterized microalgae strains
Authors: Rodríguez‐Miranda Enrique , Sánchez‐Zurano Ana , Guzmán José Luis , Acién Gabriel , Visioli Antonio 
Published: May 2022

AbstractIrradiance and temperature are among the most important variables that affect microalgae growth, being both difficult to control in outdoor raceway reactors utilized for large‐scale production of microalgae biomass. They are mainly a function of the location of the reactors, thus, producing certain strains of microalgae in inappropriate places conduces to the failure of the systems. To be able to determine important parameters of any microalgae strains on the performance of the culture, such as the influence of irradiance and temperature, is a powerful tool in decision‐making processes. In addition, whatever the strain and location, operation strategies must be defined for each specific case, such as the imposed dilution rate and culture depth, both influencing the light availability and temperature of the culture as major variables determining the biomass productivity. In this paper, a simulation‐based methodology is proposed to establish the influence of season and culture depth on the 1‐year age irradiance and temperature of the culture, and thus on the biomass productivity of different microalgae strains. Up to five of the most frequently produced strains, such as Spirulina platensis, Chlorella vulgaris, Nannochloropsis gaditana, Isochrysis galbana, and Scenedesmus almeriensis have been considered. The challenge is to develop an easy‐to‐manage decision‐making tool for the optimal design and operation of large‐scale microalgae facilities. Especially, dates for microalgae production and culture depth at which the reactors must be operated will be provided, being valid for any microalgae strain. The proposed methodology will largely contribute to the risk of investment in this field, then to enlarge the relevance of the microalgae production industry.
Uncoordinated tetrazole ligands in metal–organic frameworks for proton‐conductivity studies
Authors: Lee Daeyeon , Lee Sangho , Son Younghu , Kim Jun Yeong , Cha Seungheon , Kwak Dongmin , Lee Jooyeon , Kwak Jaesung , Yoon Minyoung , Kim Min 
Published: May 2022

AbstractZr‐based metal–organic frameworks (MOFs) were modified with pendant tetrazole ligands for proton conductivity studies. Although tetrazolate ligands coordinated to metal cations have been widely utilized to construct MOFs or porous coordination polymers, to date, the use of uncoordinated tetrazole groups in MOFs has been limited. In this study, a benzene‐1,4‐dicarboxylic acid with pendant tetrazole groups (BDC‐N4) was synthesized and used to prepare a Zr‐based UiO‐66 MOF using a mixed‐ligand strategy. The tetrazole‐functionalized UiO‐66‐N4 was compared to MOFs containing other acidic functional groups (hydroxyl, carboxylic acid, and sulfonic acid) with respect to their proton conductivities and acidities (calculated pKa). Interestingly, UiO‐66‐N4 showed a significant decrease in the activation energy with increase in the amount of added tetrazole groups, suggesting the Grotthuss proton conduction mechanism for uncoordinated tetrazole‐containing MOFs.
Green financing for sustainable development: Insights from multiple cases of Vietnamese commercial banks
Authors: Nguyen Anh Huu , Do Mai Hoang Thi , Hoang Thinh Gia , Nguyen Loan Quynh Thi 
Published: May 2022

AbstractAlthough the public sector is seen as the main party responsible for taking action on climate change and sustainable development, private commercial banks are in a unique position to support or shift the funding focus on green investment. By employing a qualitative research approach based on six commercial banks, this paper aims to investigate the current practices of how commercial banks are contributing to advance green business initiatives. Accordingly, this research examines and identifies the facilitators and challenges in domestic and foreign commercial banks in Vietnam which support green business initiatives. In addition to addressing the recent calls for the investigation of the role of commercial banks in facilitating green finance, our study expands the emerging literature by demonstrating the current efforts of Vietnam's commercial banks in fostering green finance during the Covid‐19 pandemic.
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation
Authors: Lamas Rodrigo , Ulrey Robert , Ahuja Sanjeev , Sargent Alex 
Published: May 2022

AbstractChimeric antigen receptor (CAR) T‐cell therapy is growing clinically and commercially as a powerful new approach to treat cancer. Understanding how key culture conditions such as pH and dissolved oxygen (DO) affect CAR T‐cell generation and function is important in developing better CAR‐T manufacturing processes and CAR T‐cell therapies for patients. We used the automated mini‐bioreactor (AMBR) 15 platform to assess how differences in pH and DO affect CAR T‐cell transduction, proliferation, and differentiation. We found that higher pH can significantly improve CAR T‐cell transduction and proliferation, and also biases CAR T‐cells away from an effector memory and toward a more central memory phenotype. Both high and low DO negatively affect CAR T‐cell generation, with both hypoxic and hyperoxic conditions reducing T‐cell transduction into CAR T‐cells. Collectively, this data underscores how pH and DO can significantly affect CAR T‐cell expansion and differentiation, and provides insight into the optimal culture conditions to enhance CAR T‐cell yield and phenotype in clinical and commercial processes.