bluuebunny/crossref_metadata_2025_split huggingface dataset:
Persistent immunogenicity of integrase defective lentiviral vectors delivering membrane-tethered native-like HIV-1 envelope trimers
Authors: Gallinaro Alessandra , Pirillo Maria Franca , Aldon Yoann , Cecchetti Serena , Michelini Zuleika , Tinari Antonella , Borghi Martina , Canitano Andrea , McKay Paul F. , Bona Roberta , Vescio Maria Fenicia , Grasso Felicia , Blasi Maria , Baroncelli Silvia , Scarlatti Gabriella , LaBranche Celia , Montefiori David , Klotman Mary E. , Sanders Rogier W. , Shattock Robin J. , Negri Donatella , Cara Andrea 
Published: April 2022

AbstractIntegrase Defective Lentiviral Vectors (IDLVs) represent an attractive vaccine platform for delivering HIV-1 antigens, given their ability to induce specific and persistent immune responses in both mice and non-human primates (NHPs). Recent advances in HIV-1 immunogen design demonstrated that native-like HIV-1 Envelope (Env) trimers that mimic the structure of virion-associated Env induce neutralization breadth in rabbits and macaques. Here, we describe the development of an IDLV-based HIV-1 vaccine expressing either soluble ConSOSL.UFO.664 or membrane-tethered ConSOSL.UFO.750 native-like Env immunogens with enhanced bNAb epitopes exposure. We show that IDLV can be pseudotyped with properly folded membrane-tethered native-like UFO.750 trimers. After a single IDLV injection in BALB/c mice, IDLV-UFO.750 induced a faster humoral kinetic as well as higher levels of anti-Env IgG compared to IDLV-UFO.664. IDLV-UFO.750 vaccinated cynomolgus macaques developed unusually long-lasting anti-Env IgG antibodies, as underlined by their remarkable half-life both after priming and boost with IDLV. After boosting with recombinant ConM SOSIP.v7 protein, two animals developed neutralization activity against the autologous tier 1B ConS virus mediated by V1/V2 and V3 glycan sites responses. By combining the possibility to display stabilized trimeric Env on the vector particles with the ability to induce sustained humoral responses, IDLVs represent an appropriate strategy for delivering rationally designed antigens to progress towards an effective HIV-1 vaccine.
AJILE12: Long-term naturalistic human intracranial neural recordings and pose
Authors: Peterson Steven M. , Singh Satpreet H. , Dichter Benjamin , Scheid Michael , Rao Rajesh P. N. , Brunton Bingni W. 
Published: April 2022

AbstractUnderstanding the neural basis of human movement in naturalistic scenarios is critical for expanding neuroscience research beyond constrained laboratory paradigms. Here, we describe our Annotated Joints in Long-term Electrocorticography for 12 human participants (AJILE12) dataset, the largest human neurobehavioral dataset that is publicly available; the dataset was recorded opportunistically during passive clinical epilepsy monitoring. AJILE12 includes synchronized intracranial neural recordings and upper body pose trajectories across 55 semi-continuous days of naturalistic movements, along with relevant metadata, including thousands of wrist movement events and annotated behavioral states. Neural recordings are available at 500 Hz from at least 64 electrodes per participant, for a total of 1280 hours. Pose trajectories at 9 upper-body keypoints were estimated from 118 million video frames. To facilitate data exploration and reuse, we have shared AJILE12 on The DANDI Archive in the Neurodata Without Borders (NWB) data standard and developed a browser-based dashboard.
Efficacy and safety of drug-coated balloon in the treatment of acute myocardial infarction: a meta-analysis of randomized controlled trials
Authors: Li Qiu-Yi , Chang Mei-Ying , Wang Xin-Yi , Wang An-Lu , Liu Qi-Yu , Wang Tong , Xu Hao , Chen Ke-Ji 
Published: April 2022

AbstractAcute myocardial infarction (AMI) is one of the main causes of death in the world, and the incidence of AMI is increasing in the young population. Drug-coated balloon (DCB) has become an effective concept for the treatment of in-stent restenosis, small vessel disease, bifurcation lesions, high blood risk conditions, and even de novo large vessel disease. To ensure whether DCB can play an alternative role in AMI, we conducted a comprehensive meta‐analysis of randomized controlled trials (RCTs) to evaluate the efficacy and safety of DCB in the treatment of AMI. Electronic databases were searched for RCTs that compared DCB with stent for AMI. The primary outcome was major adverse cardiac events (MACEs), the secondary outcome was late lumen loss (LLL). RevMan 5.3 software and RStudio software were used for data analysis. Five RCTs involving 528 patients with 6–12 months of follow-up were included. There was no significant difference in the incidence of MACEs between DCB group and stent group (RR, 0.85; 95% CI 0.42 to 1.74; P = 0.66). Lower LLL was shown in DCB group (WMD, − 0.29; 95% CI − 0.46 to − 0.12; P < 0.001). This meta-analysis of RCT showed that DCB might provide a promising way on AMI compared with stents. Rigorous patients’ selection and adequate predilation of culprit lesions are necessary to optimize results and prevent bailout stent implantation.PROSPERO registration number: CRD42020214333.
Heterogeneous effects of climatic conditions on Andean bean landraces and cowpeas highlight alternatives for crop management and conservation
Authors: Acosta-Quezada Pablo G. , Valladolid-Salinas Edin H. , Murquincho-Chuncho Janina M. , Jadán-Veriñas Eudaldo , Ruiz-González Mario X. 
Published: April 2022

AbstractThe use and conservation of agrobiodiversity have become critical to face the actual and future challenges imposed by climate change. Collecting phytogenetic resources is a first step for their conservation; however, the genetic material must be analysed to understand their potential to improve agricultural resilience and adaptation to the new climatic conditions. We have selected nine Phaseolus vulgaris, one P. lunatus and two Vigna unguiculata landraces from two different climatic backgrounds of the Andean region of South Ecuador and one P. vulgaris commercial cultivar, and we grew them under two different conditions of temperature and humidity (open field and greenhouse). Then, we recorded data for 32 characters of plant architecture, flower and fruit characteristics and yield, and 17 events in the phenology of the plants. We analysed the impact of treatment on species, climatic background, and each of the landraces, and identified both characters and landraces that are mostly affected by changes in their environmental conditions. Overall, higher temperatures were benign for all materials except for two P. vulgaris landraces from cold background, which performed better or developed faster under cold conditions. Finally, we calculated a climate resilience landrace index, which allowed us to classify the landraces by their plasticity to new environmental conditions, and found heterogeneous landrace susceptibility to warmer conditions. Two P. vulgaris landraces were highlighted as critical targets for conservation.
Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation
Authors: Radisch Sabine , Poltorak Mateusz P. , Wagner Michaela , Cletiu Vlad , Radisch Christian , Treise Irina , Pann Steffi , Weigt Alexis , Artner Sophie , Dreher Stefan , Fechner Fabian , Borjan Bojana , Fraessle Simon P. , Effenberger Manuel , Benke Eileen , Navratil Gottfried , Hentschel Norbert , Busch Dirk H. , Schmidt Thomas , Stemberger Christian , Germeroth Lothar 
Published: April 2022

AbstractLarge-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current standard applied for this purpose. Here, we present the transformation of previously described technology — non-magnetic immunoaffinity column chromatography-based cell selection with reversible reagents into a new clinical-grade cell isolation platform called Automated Traceless Cell affinity chromatography (ATC). ATC is a fully closed and GMP-compliant cell selection and manufacturing system. Reversibility of reagents enables (sequential) positive cell selection, optionally in combination with depletion columns, enabling capture of highly specific cell subsets. Moreover, synergy with other Streptamer-based technologies allows novel uses beyond cell isolation including integrated and automated on-column target cell activation. In conclusion, ATC technology is an innovative as well as versatile platform to select, stimulate and modify cells for clinical manufacturing and downstream therapies.
Proteome-wide landscape of solubility limits in a bacterial cell
Authors: Györkei Ádám , Daruka Lejla , Balogh Dávid , Őszi Erika , Magyar Zoltán , Szappanos Balázs , Fekete Gergely , Fuxreiter Mónika , Horváth Péter , Pál Csaba , Kintses Bálint , Papp Balázs 
Published: April 2022

AbstractProteins are prone to aggregate when expressed above their solubility limits. Aggregation may occur rapidly, potentially as early as proteins emerge from the ribosome, or slowly, following synthesis. However, in vivo data on aggregation rates are scarce. Here, we classified the Escherichia coli proteome into rapidly and slowly aggregating proteins using an in vivo image-based screen coupled with machine learning. We find that the majority (70%) of cytosolic proteins that become insoluble upon overexpression have relatively low rates of aggregation and are unlikely to aggregate co-translationally. Remarkably, such proteins exhibit higher folding rates compared to rapidly aggregating proteins, potentially implying that they aggregate after reaching their folded states. Furthermore, we find that a substantial fraction (~ 35%) of the proteome remain soluble at concentrations much higher than those found naturally, indicating a large margin of safety to tolerate gene expression changes. We show that high disorder content and low surface stickiness are major determinants of high solubility and are favored in abundant bacterial proteins. Overall, our study provides a global view of aggregation rates and hence solubility limits of proteins in a bacterial cell.
Deep learning-enabled mobile application for efficient and robust herb image recognition
Authors: Sun Xin , Qian Huinan , Xiong Yiliang , Zhu Yingli , Huang Zhaohan , Yang Feng 
Published: April 2022

AbstractWith the increasing popularity of herbal medicine, high standards of the high quality control of herbs becomes a necessity, with the herb recognition as one of the great challenges. Due to the complicated processing procedure of the herbs, methods of manual recognition that require chemical materials and expert knowledge, such as fingerprint and experience, have been used. Automatic methods can partially alleviate the problem by deep learning based herb image recognition, but most studies require powerful and expensive computation hardware, which is not friendly to resource-limited settings. In this paper, we introduce a deep learning-enabled mobile application which can run entirely on common low-cost smartphones for efficient and robust herb image recognition with a quite competitive recognition accuracy in resource-limited situations. We hope this application can make contributions to the increasing accessibility of herbal medicine worldwide.
Genome-wide identification, classification and expression analysis of the JmjC domain-containing histone demethylase gene family in Jatropha curcas L.
Authors: Wang Jie , Jiang Xiaoke , Bai Hanrui , Liu Changning 
Published: April 2022

AbstractJmjC domain-containing proteins, an important family of histone lysine demethylase, play significant roles in maintaining the homeostasis of histone methylation. In this study, we comprehensively analyzed the JmjC domain-containing gene family in Jatropha curcas and found 20 JmjC domain-containing genes (JcJMJ genes). Phylogenetic analysis revealed that these JcJMJ genes can be classified into five major subgroups, and genes in each subgroup had similar motif and domain composition. Cis-regulatory element analysis showed that the number and types of cis-regulatory elements owned by the promoter of JcJMJ genes in different subgroup were significantly different. Moreover, miRNA target prediction result revealed a complicated miRNA-mediated post-transcriptional regulatory network, in which JcJMJ genes were regulated by different numbers and types of miRNAs. Further analysis of the tissue and stress expression profiles showed that many JcJMJ genes had tissue and stress expression specificity. All these results provided valuable information for understanding the evolution of JcJMJ genes and the complex transcriptional and post transcriptional regulation involved, and laid the foundation for further functional analysis of JcJMJ genes.
Employing computational fluid dynamics technique for analyzing the PACK-1300XY with methanol and isopropanol mixture
Authors: Cao Y. , Dhahad H. A. , Khandakar A. , Chowdury M. E. H. , Ayari M. A. , Alizadeh S. M. , Vaferi B. 
Published: April 2022

AbstractIn this study, an innovative wire gauze structured packing, namely PACK-1300XY with a specific surface area of 1300 m2/m3 has been characterized by performing computational fluid dynamics (CFD) approach. Indeed, different features of this packing (height equivalent to a theoretical plate, wet/dry pressure drop, and mass transfer efficiency) were analyzed by analyzing the flow regime using the three-dimensional CFD approach with the Eulerian–Eulerian multiphase scenario. The results showed the mean relative deviation of 16% (for wet pressure drop), 14% (for dry pressure drop), and 17% (for mass transfer efficiency) between the CFD predictions and experimental measurements. These excellent levels of consistency between the numerical findings and experimental observations approve the usefulness of the CFD-based approach for reliable simulation of separation processes.
Comparison of vision-related quality of life in patients with homonymous hemianopia and monocular blindness
Authors: Choi Hee-young , Kim Su-Jin , Kim Sang Yoon , Lee Ji-Eun 
Published: April 2022

AbstractTo evaluate the vision-related quality of life (QoL) in patients with homonymous hemianopia (HH). The study compared the QoL in 32 patients with HH and 33 patients with monocular blindness. Best-corrected visual acuity (BCVA) and visual field test were investigated. The National Eye Institute-Visual Function Questionnaire 25 (NEI VFQ-25) and independent mobility questionnaires (IMQs) were used to assess their perceived visual and physical functioning abilities. The results of QoL questionnaires were compared in two groups. The mean deviation (MD) in the better eye was significantly lower in the HH group than in the monocular blindness group. The composite scores of NEI-VFQ and IMQs were significantly lower in the HH patients than in the monocular blindness patients. The driving-related score was significantly lower in patients with right hemianopsia than in those with left hemianopsia. The outdoor activity-related score was significantly lower in patients aged less than 55 years than in patients aged 55 years and more. Homonymous hemianopia had a negative impact on patients’ QoL by limiting their vision related activities compared to monocular blindness. The MD of the better eye in the HH patients reflects the binocular visual field and can affect the real visual function and QoL.
Controlling the threshold voltage of a semiconductor field-effect transistor by gating its graphene gate
Authors: Anzi Luca , Tuktamyshev Artur , Fedorov Alexey , Zurutuza Amaia , Sanguinetti Stefano , Sordan Roman 
Published: April 2022

AbstractThe threshold voltage of a field-effect transistor (FET) determines its switching and limits the scaling of the supply voltage in the logic gates. Here we demonstrate a GaAs FET with a monolayer graphene gate in which the threshold voltage was externally controlled by an additional control gate. The graphene gate forms a Schottky junction with the transistor channel, modulating the channel conductivity. The control gate sets the work function of the graphene gate, controlling the Schottky barrier height and therefore the threshold voltage, and reduces the subthreshold swing down to ~60 mV dec−1. The change of the threshold voltage was large enough to turn the initially depletion mode FETs into the enhancement mode FETs. This allowed to realize logic gates with a positive switching threshold in which the threshold voltage of each transistor was independently set. The presented FETs can also be operated as dual-gate FETs, which was demonstrated by realizing frequency mixers.
A 2D/1D heterojunction nanocomposite built from polymeric carbon nitride and MIL-88A(Fe) derived α-Fe2O3for enhanced photocatalytic degradation of rhodamine B
Authors: Viswanathan Vandana P. , Nayarassery Adarsh N. , Xavier Marilyn Mary , Mathew Suresh 
Published: April 2022

2D/1D heterojunction α-Fe2O3/C3N4 photocatalysts containing α-Fe2O3 microrods and polymeric carbon nitride flakes are synthesised through the calcination of Fe-based metal-organic frameworks and boost the visible light photocatalytic degradation of rhodamine B.
Popcorn-like Co3O4 nanoparticles confined in a three-dimensional hierarchical N-doped carbon nanotube network as a highly-efficient trifunctional electrocatalyst for zinc–air batteries and water splitting devices
Authors: Yao Xiuyun , Wang Xueying , Sun Lixin , Li Ling , Kan Erjun , Ouyang Bo , Zhang Wenming 
Published: April 2022

A novel unique 3D hierarchical structural electrocatalyst is synthesized, where popcorn-like Co3O4 nanoparticles coated with N-doped amorphous carbon anchor onto the tips of N-doped carbon nanotubes (NCNTs), and the NCNTs grow on carbon nanofiber.
A missing link in the nitrogen-rich organic chain on Titan
Authors: Carrasco N. , Bourgalais J. , Vettier L. , Pernot P. , Giner E. , Spezia R. 
Published: April 2022

Context.The chemical building blocks of life contain a large proportion of nitrogen, an essential element. Titan, the largest moon of Saturn, with its dense atmosphere of molecular nitrogen and methane, offers an exceptional opportunity to explore how this element is incorporated into carbon chains through atmospheric chemistry in our Solar System. A brownish dense haze is consistently produced in the atmosphere and accumulates on the surface on the moon. This solid material is nitrogen-rich and may contain prebiotic molecules carrying nitrogen.Aims.To date, our knowledge of the processes leading to the incorporation of nitrogen into organic chains has been rather limited. In the present work, we investigate the formation of nitrogen-bearing ions in an experiment simulating Titan’s upper atmosphere, with strong implications for the incorporation of nitrogen into organic matter on Titan.Methods.By combining experiments and theoretical calculations, we show that the abundant N2+ion, produced at high altitude by extreme-ultraviolet solar radiation, is able to form nitrogen-rich organic species.Results.An unexpected and important formation of CH3N2+and CH2N2+diazo-ions is experimentally observed when exposing a gas mixture composed of molecular nitrogen and methane to extreme-ultraviolet radiation. Our theoretical calculations show that these diazo-ions are mainly produced by the reaction of N2+with CH3radicals. These small nitrogen-rich diazo-ions, with a N/C ratio of two, appear to be a missing link that could explain the high nitrogen content in Titan’s organic matter. More generally, this work highlights the importance of reactions between ions and radicals, which have rarely been studied thus far, opening up new perspectives in astrochemistry.
An Efficient Route to Branched Allylsilanes through Copper-Catalyzed Allene Hydrosilylation Using Readily Available Silanes
Authors: Liu Xiao-Tao , Zhan Zhuang-Ping , Chen Jun-Jia 
Published: April 2022

AbstractA CuCl-catalyzed hydrosilylation of allenes with PhSiH3 has been developed. It is the first example of using readily available cheap silane reagents to generate branched allylsilanes in copper-catalyzed allene hydrosilylation. This base-metal catalyst offered an atom-economical and efficient route to branched allylsilanes with excellent regioselectivity.
Pyrazoles in the Intersection of Mesomeric Betaines and N-Heterocyclic Carbenes: Formation of NHC Selenium Adducts of Pyrazolium-4-aminides
Authors: Schmidt Andreas , Hillrichs Kai , Namyslo Jan C. , Lederle Felix , Hübner Eike G. 
Published: April 2022

AbstractStarting from 4-nitropyrazole, eight mesoionic pyrazolium-4-aminides were prepared by a six-step reaction sequence. The deprotonation of 1,2-disubstituted 4-amido-1H-pyrazolium salts by an anion exchange resin in its hydroxide form is the final step of the synthesis. A tautomeric equilibrium between the mesoionic compounds (pyrazolium-4-aminides) and N-heterocyclic carbenes (pyrazol-3-ylidenes) can be formulated; however, the NHC tautomers were not detected by means of NMR spectroscopy in polar aprotic solvents such as DMSO-d
6 or MeCN-d
3. Apart from tautomerism, anionic N-heterocyclic carbenes can be formulated as a result of a deprotonation of the mesoionic compounds­. Trapping reactions were performed with selenium, which resulted in the formation of pyrazole-3-selenones. Methylation at the selenium atom gave the corresponding 3-(methylselanyl)-4-amido-1H-pyrazolium salts, which were deprotonated to give new mesomeric betaines, 3-(methylselanyl)-1H-pyrazolium-4-aminides as unique compounds­. DFT-calculations as well as 77Se NMR spectroscopic measurements were carried out.
Achalasia Cardia: Balloon, Tunnel, or Knife?
Authors: Maydeo Amit 
Published: April 2022

AbstractAchalasia cardia is a rare esophageal motility disorder characterized by impaired relaxation of the lower esophageal sphincter during swallowing and aperistalsis of the esophageal smooth muscles. The treatment approaches to achalasia include nonsurgical treatment with medications (nitrates, calcium channel blockers), endoscopic treatment (balloon pneumatic dilation [PD], botulinum toxin injection [BTI], peroral endoscopic myotomy [POEM]), and surgery (laparoscopic Heller's myotomy [LHM]). The subtype of achalasia (the Chicago Classification) governs the ideal treatment. For the commonly encountered achalasia subtype I and II, PD, LHM, and POEM all have similar efficacy. However, for type III achalasia, POEM seems to be the best line of treatment. Among high-risk elderly patients or those with comorbidities, BTI is preferred. The choice of treatment should be minimally invasive therapy with good short-term and sustained long-term effects with negligible adverse events. POEM seems to be evolving as a first-line therapy among the available therapies. Here, we review the treatment options among achalasia cardia patients with special attention to post-POEM gastroesophageal reflux disease and its management.
Percutaneous Cholecystostomy: A Bridge to Less Morbidity
Authors: Singh Anil Kumar 
Published: April 2022

AbstractPercutaneous cholecystostomy (PC) is a minimally invasive procedure for decompressing gall bladder (GB) or biliary system in emergency settings, performed in patients with GB or biliary diseases who are at high risk for surgical exploration. Indications range from acute cholecystitis in seriously ill patients to overdistended GB with impending perforation to overt GB perforation. This procedure, by allowing biliary drainage, helps in controlling the infection and optimizing the patient's condition for definitive treatment in the form of elective surgery if possible, thus acting as a bridge to a definitive treatment option. In some cases, such as acute acalculous cholecystitis, it may obviate the need for surgery, and in malignant biliary obstruction, it may be used as a palliative measure to keep GB decompressed. This review article focuses on and revisits many aspects of PC including technical aspects, clinical indications, outcomes, and safety of the procedure, in addition to its role as bridge therapy versus definitive therapy versus palliative option. It includes observations based on the author's own work experience and review of the literature.