bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput phenotyping allows the selection of soybean genotypes for earliness and high grain yield
Authors: Santana Dthenifer Cordeiro , de Oliveira Cunha Marcos Paulo , dos Santos Regimar Garcia , Cotrim Mayara Fávero , Teodoro Larissa Pereira Ribeiro , da Silva Junior Carlos Antonio , Baio Fabio Henrique Rojo , Teodoro Paulo Eduardo 
Published: February 2022

AbstractBackgroundPrecision agriculture techniques are widely used to optimize fertilizer and soil applications. Furthermore, these techniques could also be combined with new statistical tools to assist in phenotyping in breeding programs. In this study, the research hypothesis was that soybean cultivars show phenotypic differences concerning wavelength and vegetation index measurements.ResultsIn this research, we associate variables obtained via high-throughput phenotyping with the grain yield and cycle of soybean genotypes. The experiment was carried out during the 2018/2019 and 2019/2020 crop seasons, under a randomized block design with four replications. The evaluated soybean genotypes included 7067, 7110, 7739, 8372, Bonus, Desafio, Maracai, Foco, Pop, and Soyouro. The phenotypic traits evaluated were: first pod height (FPH), plant height (PH), number of branches (NB), stem diameter (SD), days to maturity (DM), and grain yield (YIE). The spectral variables evaluated were wavelengths and vegetation indices (NDVI, SAVI, GNDVI, NDRE, SCCCI, EVI, and MSAVI). The genotypes Maracai and Foco showed the highest grain yields throughout the crop seasons, in addition to belonging to the groups with the highest means for all VIs. YIE was positively correlated with the NDVI and certain wavelengths (735 and 790 nm), indicating that genotypes with higher values for these spectral variables are more productive. By path analyses, GNDVI and NDRE had the highest direct effects on the dependent variable DM, while NDVI had a higher direct effect on YIE.ConclusionsOur findings revealed that early and productive genotypes can be selected based on vegetation indices and wavelengths. Soybean genotypes with a high grain yield have higher means for NDVI and certain wavelengths (735 and 790 nm). Early genotypes have higher means for NDRE and GNDVI. These results reinforce the importance of high-throughput phenotyping as an essential tool in soybean breeding programs.
Spinopelvic mobility is influenced by pre-existing contralateral hip arthroplasty: a matched-pair analysis in patients undergoing hip replacement
Authors: Muellner Maximilian , Becker Luis , Wang Zhen , Hu Zhouyang , Hardt Sebastian , Pumberger Matthias , Haffer Henryk 
Published: February 2022

Abstract
Background
Spinopelvic mobility gained increased attention as a contributing factor for total hip arthroplasty (THA) instability. However, it is unknown how a pre-existing THA affects spinopelvic mobility. Therefore, a propensity-score-matched analysis of primary THA patients comparing the individual segments of spinopelvic mobility between patients with pre-existing THA and no-existing THA was conducted. Consequently, the study aimed to discuss (1) whether patients with a pre-existing THA have altered spinopelvic mobility compared to the control group and (2) if spinopelvic mobility changes after THA.

Methods
A prospective observational study enrolled 197 elective primary THA patients, including N = 44 patients with a pre-existing unilateral THA. Using propensity-score matching adapted for age, sex, and BMI, N = 44 patients without a pre-existing THA were determined. The patients received stereoradiography in standing and relaxed sitting position pre- and postoperatively. Assessed parameters were lumbar lordosis (LL), pelvic tilt (PT), and pelvic femoral angle (PFA). Key parameters of the spinopelvic mobility were defined as lumbar flexibility (∆LL = LLstanding − LLsitting), pelvic mobility (∆PT = PTstanding − PTsitting) and hip motion (∆PFA = PFAstanding − PFAsitting). Pelvic mobility was classified as stiff (∆PT < 10°), normal (∆PT ≥ 10°–30°) and hypermobile (∆PT > 30°). The Wilcoxon rank sum test for dependent samples was used.

Results
Pelvic mobility was significantly increased in the pre-existing THA group (∆PT 18.2° ± 10.7) compared to the control group (∆PT 7.7° ± 8.0; p < 0.001) preoperatively and postoperatively (pre-existing: 22.2° ± 9.3; control: 17.0° ± 9.2, p = 0.022). Lumbar flexibility was significantly increased in the pre-existing THA group (∆LL 21.6° ± 11.8) compared to the control group (∆LL 12.4° ± 7.8; p < 0.001) preoperatively and postoperatively (pre-existing: 25.7° ± 11.0; control: 19.0° ± 10.2; p = 0.011). The contribution of stiff pelvic mobility is distinctly smaller in the pre-existing THA group (25%) than in the control group (75%) preoperatively.

Conclusions
Pre-existing THA is associated with significantly enhanced pelvic mobility and lumbar flexibility. Accordingly, we identified the patients without a pre-existing THA as risk candidates with higher likelihood for pathological spinopelvic mobility. This information will assist arthroplasty surgeons in deciding which THA candidates require preoperative radiological screening for pathologic spinopelvic mobility.

Level of evidence
Level II prospective cohort study.
Effectiveness of motor imagery for improving functional performance after total knee arthroplasty: a systematic review with meta-analysis
Authors: Li Ran , Du Jubao , Yang Kun , Wang Xue , Wang Wenjiao 
Published: February 2022

Abstract
Background
The aim of this study was to appraise the effects of motor imagery on the functional performance improvement among total knee arthroplasty patients systematically. We hypothesized a relatively greater recovery in the motor imagery group.

Methods
Medline (Ovid), Embase and Cochrane Controlled Register of Trials (CENTRAL) were searched from inception to October 1st, 2021. We included randomized controlled trials evaluating the effects of motor imagery on the functional recovery among total knee arthroplasty patients. Measurements included range of motion, strength intensity, Visual Analogue Scale, Time Up and Go Test, Oxford Knee Score, Western Ontario and McMaster Universities Osteoarthritis Index, all of which were evaluated before and after intervention. Mean differences (MD) or standard mean differences (SMD) and 95% confidence intervals (CI) were calculated. The Cochrane risk of bias tool was used to assess the risk of bias.

Results
Six studies with 168 patients were included for the meta-analysis. The SMD of strength intensity was increased (SMD = 0.90, 95% CI = [0.47]–[1.32], P < 0.001). The SMD of Visual Analogue Scale was reduced (SMD =  − 0.91; 95% CI = [− 1.29]–[− 0.52], P < 0.001). The SMD of Time Up and Go Test was reduced (SMD =  − 0.56, 95% CI = [− 0.94]–[− 0.19], P = 0.003). The MD of Oxford Knee Score was slightly increased (MD = 0.79-point, 95% CI = [− 0.31]–[1.88], P = 0.159). The outcomes of range of motion, Western Ontario and McMaster Universities Osteoarthritis Index were described according to the original data.

Conclusion
Compared with control therapy, motor imagery in the intervention group achieved an effective treatment for strength enhancement, pain reduction and physical activities improvement. More large-scale, prospective researches are needed in the future.
Trial registration: The PROSPERO trial registration number is CRD42021250996.
Pegs not superior to screws for fixation of fractures of the proximal humerus
Authors: Bønes Ingrid , Karlberg Anna Cecilie , Liljeholm Maria , Fraser Alexander Nilsskog , Madsen Jan Erik , Fjalestad Tore 
Published: February 2022

AbstractBackgroundAngular stable plates were introduced two decades ago as a promising treatment for fixation of displaced fractures of the proximal humerus (PHF). However, high rates of adverse events and reoperations have been reported. One frequent reason is secondary penetration of screws into the glenohumeral joint, due to sinking of the fracture or avascular head necrosis. To prevent joint penetrations angular stable plates with smooth locking pegs instead of locking screws have been developed. The aim of the present study was to investigate whether blunt pegs instead of pointed screws reduced the risk of secondary penetration into the glenohumeral joint during fracture healing after operatively treated PHFs.MethodsFrom two different patient cohorts with displaced PHFs (60 treated with PHILOS plate with screws and 50 with ALPS-PHP plate with pegs), two groups were matched according to fracture type AO/OTA 11-B2 and 11-C2 and age (55–85 years). They were followed up at 3, 6 and 12 months. Primary outcome was radiographic signs of peg or screw penetrations into the glenohumeral joint at 12 months. Secondary outcomes were Oxford shoulder score (OSS) and Constant Score (CS) and radiographic signs of avascular humeral head necrosis (AVN).ResultsEighteen PHILOS patients with B2 and C2 fractures could be matched with a corresponding group of 18 operated with ALPS-PHP with pegs. The number of penetrations of pegs and screws were equal between the two groups and the development of avascular head necrosis did not differ either. The functional outcomes for both OSS and CS at 12 months was clearly in favor of patients without joint penetrations in both groups.ConclusionWe found no differences in the number of screw or peg penetrations in the PHILOS and ALPS-PHP group and the occurrence of AVN was equal. Joint penetrations led to inferior functional outcomes at 1 year.The ClinicalTrials.gov identifier 20/11/12 prospectively for the Philos Group is NCT01737060, and for the ALPS group 11/03/20 retrospectively is NCT04622852.
3D-printed porous Ti6Al4V scaffolds for long bone repair in animal models: a systematic review
Authors: Gu Yifei , Sun Yi , Shujaat Sohaib , Braem Annabel , Politis Constantinus , Jacobs Reinhilde 
Published: February 2022

Abstract
Background
Titanium and its alloys have been widely employed for bone tissue repair and implant manufacturing. The rapid development of three-dimensional (3D) printing technology has allowed fabrication of porous titanium scaffolds with controllable microstructures, which is considered to be an effective method for promoting rapid bone formation and decreasing bone absorption. The purpose of this systematic review was to evaluate the osteogenic potential of 3D-printed porous Ti6Al4V (Ti64) scaffold for repairing long bone defects in animal models and to investigate the influential factors that might affect its osteogenic capacity.

Methods
Electronic literature search was conducted in the following databases: PubMed, Web of Science, and Embase up to September 2021. The SYRCLE's tool and the modified CAMARADES list were used to assess the risk of bias and methodological quality, respectively. Due to heterogeneity of the selected studies in relation to protocol and outcomes evaluated, a meta-analysis could not be performed.

Results
The initial search revealed 5858 studies. Only 46 animal studies were found to be eligible based on the inclusion criteria. Rabbit was the most commonly utilized animal model. A pore size of around 500–600 µm and porosity of 60–70% were found to be the most ideal parameters for designing the Ti64 scaffold, where both dodecahedron and diamond pores optimally promoted osteogenesis. Histological analysis of the scaffold in a rabbit model revealed that the maximum bone area fraction reached 59.3 ± 8.1% at weeks 8–10. Based on micro-CT assessment, the maximum bone volume fraction was found to be 34.0 ± 6.0% at weeks 12.

Conclusions
Ti64 scaffold might act as a promising medium for providing sufficient mechanical support and a stable environment for new bone formation in long bone defects.
Trail registration The study protocol was registered in the PROSPERO database under the number CRD42020194100.
Operational assessment tool for forest carbon dynamics for the United States: a new spatially explicit approach linking the LUCAS and CBM-CFS3 models
Authors: Sleeter Benjamin M. , Frid Leonardo , Rayfield Bronwyn , Daniel Colin , Zhu Zhiliang , Marvin David C. 
Published: February 2022

AbstractBackgroundQuantifying the carbon balance of forested ecosystems has been the subject of intense study involving the development of numerous methodological approaches. Forest inventories, processes-based biogeochemical models, and inversion methods have all been used to estimate the contribution of U.S. forests to the global terrestrial carbon sink. However, estimates have ranged widely, largely based on the approach used, and no single system is appropriate for operational carbon quantification and forecasting. We present estimates obtained using a new spatially explicit modeling framework utilizing a “gain–loss” approach, by linking the LUCAS model of land-use and land-cover change with the Carbon Budget Model of the Canadian Forest Sector (CBM-CFS3).ResultsWe estimated forest ecosystems in the conterminous United States stored 52.0 Pg C across all pools. Between 2001 and 2020, carbon storage increased by 2.4 Pg C at an annualized rate of 126 Tg C year−1. Our results broadly agree with other studies using a variety of other methods to estimate the forest carbon sink. Climate variability and change was the primary driver of annual variability in the size of the net carbon sink, while land-use and land-cover change and disturbance were the primary drivers of the magnitude, reducing annual sink strength by 39%. Projections of carbon change under climate scenarios for the western U.S. find diverging estimates of carbon balance depending on the scenario. Under a moderate emissions scenario we estimated a 38% increase in the net sink of carbon, while under a high emissions scenario we estimated a reversal from a net sink to net source.ConclusionsThe new approach provides a fully coupled modeling framework capable of producing spatially explicit estimates of carbon stocks and fluxes under a range of historical and/or future socioeconomic, climate, and land management futures.
Selective reduction of astrocyte apoE3 and apoE4 strongly reduces Aβ accumulation and plaque-related pathology in a mouse model of amyloidosis
Authors: Mahan Thomas E. , Wang Chao , Bao Xin , Choudhury Ankit , Ulrich Jason D. , Holtzman David M. 
Published: February 2022

Abstract
Background
One of the key pathological hallmarks of Alzheimer disease (AD) is the accumulation of the amyloid-β (Aβ) peptide into amyloid plaques. The apolipoprotein E (APOE) gene is the strongest genetic risk factor for late-onset AD and has been shown to influence the accumulation of Aβ in the brain in an isoform-dependent manner. ApoE can be produced by different cell types in the brain, with astrocytes being the largest producer of apoE, although reactive microglia also express high levels of apoE. While studies have shown that altering apoE levels in the brain can influence the development of Aβ plaque pathology, it is not fully known how apoE produced by specific cell types, such as astrocytes, contributes to amyloid pathology.

Methods
We utilized APOE knock-in mice capable of having APOE selectively removed from astrocytes in a tamoxifen-inducible manner and crossed them with the APP/PS1-21 mouse model of amyloidosis. We analyzed the changes to Aβ plaque levels and assessed the impact on cellular responses to Aβ plaques when astrocytic APOE is removed.

Results
Tamoxifen administration was capable of strongly reducing apoE levels in the brain by markedly reducing astrocyte apoE, while microglial apoE expression remained. Reduction of astrocytic apoE3 and apoE4 led to a large decrease in Aβ plaque deposition and less compact plaques. While overall Iba1+ microglia were unchanged in the cortex after reducing astrocyte apoE, the expression of the disease-associated microglial markers Clec7a and apoE were lower around amyloid plaques, indicating decreased microglial activation. Additionally, astrocyte GFAP levels are unchanged around amyloid plaques, but overall GFAP levels are reduced in the cortex of female apoE4 mice after a reduction in astrocytic apoE. Finally, while the amount of neuritic dystrophy around remaining individual plaques was increased with the removal of astrocytic apoE, the overall amount of cortical amyloid-associated neuritic dystrophy was significantly decreased.

Conclusion
This study reveals an important role of astrocytic apoE3 and apoE4 on the deposition and accumulation of Aβ plaques as well as on certain Aβ-associated downstream effects.
Transition age youth mental health: addressing the gap with telemedicine
Authors: Khetarpal Susheel K. , Auster Lauren S. , Miller Elizabeth , Goldstein Tina R. 
Published: February 2022

AbstractTransition age youth (TAY), a demographic spanning ages 15–26, navigate a myriad of developmental transitions, ranging from identity formation and intimate relationships to substance use. Unfortunately, many young adults continue to have a dearth of mental health services and programing tailored to their unique developmental needs. Moreover, the systems of care in place are generally designed for treating traditional pediatric and adult patients but not ideally suited to meet the needs of TAY. Given the additional stressors from the COVID-19 pandemic, TAY are now, more than ever, in need of routine mental health care. We posit that the rapid expansion of telemedicine programming developed in response to the pandemic could be beneficial in mitigating this historic gap in care. In this commentary, we call on mental health providers and researchers to expand and invest in the growing number of telemedicine interventions and programming for this population so that TAY can begin to receive the care they so desperately need.
HDAC6 involves in regulating the lncRNA-microRNA-mRNA network to promote the proliferation of glioblastoma cells
Authors: Wu An-Chih , Yang Wen-Bin , Chang Kwang-Yu , Lee Jung-Shun , Liou Jing-Ping , Su Ruei-Yuan , Cheng Siao Muk , Hwang Daw-Yang , Kikkawa Ushio , Hsu Tsung-I , Wang Chih-Yang , Chang Wen-Chang , Chen Pin-Yuan , Chuang Jian-Ying 
Published: February 2022

Abstract
Background
Glioblastoma (GBM) is the most aggressive and lethal brain tumor. Although the histone deacetylase (HDAC)/transcription factor axis promotes growth in GBM, whether HDACs including HDAC6 are involved in modulating long non-coding RNAs (lncRNAs) to affect GBM malignancy remains obscure.

Methods
Integrative analysis of microarray and RNA-seq was performed to identify lncRNAs governed by HDAC6. Half-life measurement and RNA-protein pull-down assay combined with isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis were conducted to identify RNA modulators. The effect of LINC00461 on GBM malignancy was evaluated using animal models and cell proliferation-related assays. Functional analysis of the LINC00461 downstream networks was performed comprehensively using ingenuity pathway analysis and public databases.

Results
We identified a lncRNA, LINC00461, which was substantially increased in stem-like/treatment-resistant GBM cells. LINC00461 was inversely correlated with the survival of mice-bearing GBM and it was stabilized by the interaction between HDAC6 and RNA-binding proteins (RBPs) such as carbon catabolite repression—negative on TATA-less (CCR4-NOT) core exoribonuclease subunit 6 and fused in sarcoma. Targeting LINC00461 using azaindolylsulfonamide, an HDAC6 inhibitor, decreased cell-division-related proteins via the lncRNA-microRNA (miRNA)-mRNA networks and caused cell-cycle arrest, thereby suppressing proliferation in parental and drug-resistant GBM cells and prolonging the survival of mice-bearing GBM.

Conclusions
This study sheds light on the role of LINC00461 in GBM malignancy and provides a novel therapeutic strategy for targeting the HDAC6/RBP/LINC00461 axis and its downstream effectors in patients with GBM.
Evaluating statistical approaches to define clonal origin of tumours using bulk DNA sequencing: context is everything
Authors: Kader Tanjina , Zethoven Magnus , Gorringe Kylie L. 
Published: February 2022

AbstractClonal analysis of tumour sequencing data enables the evaluation of the relationship of histologically distinct synchronous lesions, such as co-existing benign areas, and temporally distinct tumours, such as primary-recurrence comparisons. In this review, we summarise statistical approaches that are commonly employed to define tumour clonal relatedness using data from bulk DNA technologies. We discuss approaches using total copy number, allele-specific copy number and mutation data, and the relative genomic resolution required for analysis and summarise some of the current tools for inferring clonal relationships. We argue that the impact of the biological context is critical in selecting any particular approach, such as the relative genomic complexity of the lesions being compared, and we recommend considering this context before employing any method to a new dataset.
Treatment of Impetigo with Antiseptics—Replacing Antibiotics (TIARA) trial: a single blind randomised controlled trial in school health clinics within socioeconomically disadvantaged communities in New Zealand
Authors: Primhak Sarah , Gataua Alicia , Purvis Diana , Thompson John M. D. , Walker Cameron , Best Emma , Leversha Alison 
Published: February 2022

AbstractBackgroundImpetigo is a common and contagious bacterial skin infection, affecting children worldwide, but it is particularly prevalent in socioeconomically disadvantaged communities. In New Zealand, widespread prescribing of the topical antibiotic fusidic acid had led to an increase in antimicrobial resistance ofStaphylococcus aureus. Alternative treatments are urgently being sought, and as impetigo is a superficial infection, it has been suggested that topical antiseptics such as hydrogen peroxide or simple wound care alone may treat impetigo while avoiding the risk of increased antimicrobial resistance.MethodsThis protocol for a non-inferiority, single-blind randomised controlled trial compares topical fusidic acid with topical hydrogen peroxide and with simple wound care in the treatment of childhood impetigo. Participants are randomised to one of the three treatments for 5 days. The primary outcome is clinical improvement assessed through paired photographs analysed by graders blinded to treatment arm. The trial is based in school health clinics in an urban centre in New Zealand. Comparison of antimicrobial resistance patterns pre- and post-treatment is also performed.DiscussionSpecial note is made of the need to involve the communities most affected by impetigo in the design and implementation of the clinical trial to recruit the children most in need of safe and effective treatments.Trial registrationAustralian New Zealand Clinical Trials Registry (ANZCTR)12616000356460. Registered on March 10, 2016 Protocol amendment number: 05EB and AL contributed equally as senior authors.