bluuebunny/crossref_metadata_2025_split huggingface dataset:
KIAA1199 drives immune suppression to promote colorectal cancer liver metastasis by modulating neutrophil infiltration
Authors: Wang Haihong , Zhang Biying , Li Ruiqi , Chen Jiayuan , Xu Guojie , Zhu Ying , Li Jiao , Liang Qing , Hua Qingling , Wang Lanqing , Wen Lu , Jin Min , Fan Jun , Zhang Dejun , Zhao Lei , Yu Dandan , Lin Zhenyu , Ren Jinghua , Zhang Tao 
Published: February 2022

Abstract

Background and Aims
Metastasis is the primary cause of cancer mortality, and colorectal cancer (CRC) frequently metastasizes to the liver. Our previous studies demonstrated the critical role of KIAA1199 in tumor invasion and metastasis in CRC. In the present study, we described an immune regulatory effect of KIAA1199 that creates a permissive environment for metastasis.

Approach and Results
Flow cytometry was used to examine the effects of KIAA1199 on the infiltration of tumor immune cells. Neutrophils and T cells were isolated, stimulated, and/or cultured for in vitro function assays. In the patients with CRC, high expression levels of KIAA1199 were associated with an increased neutrophil infiltration into the liver. This result was further validated in mouse metastasis models. The increased influx of neutrophils contributed to the KIAA1199‐driven CRC liver metastasis. Mechanistically, KIAA1199 activated the TGFβ signaling pathway by interacting with the TGFBR1/2 to stimulate CXCL1 and CXCL3 production, thereby driving the aggregation of immunosuppressive neutrophils. Genetic blockade or pharmacologic inhibition of KIAA1199 restored tumor immune infiltration, impeded tumor progression, and potentiated response to immune checkpoint blockade (ICB).

Conclusions
These findings indicated that KIAA1199 could facilitate the liver infiltration of immunosuppressive neutrophils via the TGFβ–chemokine (C‐X‐C motif) ligand (CXCL)3/1–CXCR2 axis, which might be clinically targeted for the treatment of hepatic metastasis.
Usability Analysis of an MBSE Model of Standard Operating Procedures
Authors: Bashatah Jomana , Sherry Lance 
Published: February 2022

AbstractStandard Operating Procedures (SOPs) are the “glue” that holds the operator‐plant system together throughout the missions performed by the system. For this reason, developing and modeling the SOPs early in the development life cycle, can inform the design, and reduce development and operational costs accrued due to sub‐ optimal operator‐plant interaction. This paper describes how to apply the popular Heuristic Evaluation method for usability analysis on SOPs modeled using Action Diagrams in a Model‐based System Engineering (MBSE) development environment.
The pieces of the smallholder puzzle: The add‐in role of farmers' characteristics in irrigation improvement projects in the old lands of Egypt
Authors: Salman Maher , Fertő Imre , Pék Éva , El‐Desouky Ibrahim Abd Al‐Latif , Zaki Saad , Darwesh Reda , Lebdi Fethi , Alali Qotada 
Published: February 2022

AbstractIrrigation development is essential for reducing vulnerability to water resource scarcity, food insecurity, and climate change impacts in Egypt. The centralized irrigation development must, however, overcome the challenges of the diversity of smallholders and the pre‐existing, individualistic irrigation management. The purpose of this paper is to examine the farmers' characteristics that influence the design and success of irrigation improvement programmes in the Old Lands of Egypt. The paper investigates this issue from the perspectives of farmers by analysing farm features, agricultural practices, irrigation practices, and satisfaction with water services. The clustering method is applied to create farmer groups within the geographical area of the programme and seek consistent differences that affect the outcome of irrigation improvement. The results show that farmers are less diverse in their agrotechnology and more heterogenous in terms of capacities, livelihood sources, and land tenure structures. The article argues that grouping methods to deploy improved irrigation infrastructure should give more consideration to the farmers' individual features. Programmes intended to replicate such irrigation development or leverage the existing results should address these particularities to create a socially adaptive environment for future irrigation investment in Egypt.
Engineer a pre‐metastatic niched microenvironment to attract breast cancer cells by utilizing a 3D printed polycaprolactone/nano‐hydroxyapatite osteogenic scaffold – An in vitro model system for proof of concept
Authors: Xiong Qisheng , Zhang Ningze , Zhang Miaomiao , Wang Meng , Wang Lizhen , Fan Yubo , Lin Chia‐Ying 
Published: February 2022

AbstractBreast cancer bone metastasis is not a random process. It is affected by the local microenvironment which determines the propensity of cancer cells to invade and colonize into the secondary sites. This microenvironment is termed a pre‐metastatic niche. With the flexibility to incorporate different biofactors, tissue‐engineering scaffolds provide an advantageous environment to promote “designed” osteogenesis that may mimic the bony pre‐metastatic niche. In the current study, designed polycaprolactone (PCL) scaffolds enriched with nano‐hydroxyapatite (nHA) were fabricated through three‐dimensional (3D) printing. Subsequently, human mesenchymal stem cells (hMSCs) were seeded onto PCL‐nHA scaffolds for osteogenic differentiation to establish the pre‐metastatic niched microenvironment. Furthermore, transwell migration assay was used to investigate recruitment of MDA‐MB‐231, MCF‐7, and MDA‐MB‐453 breast cancer cells to the osseous PCL‐nHA scaffolds. Our results showed that the mRNA levels of alkaline phosphatase (ALP), runt‐related transcription factor 2 (Runx2), and osteocalcin (OCN) of hMSCs on the PCL‐nHA scaffolds were dramatically increased compared those with the PCL scaffolds (control) at day 7, 14, and 28. Meanwhile, the migration analysis showed that the higher maturation of osteogenesis and bone metabolism collectively contributed to the creation of a more favorable niched site for the cancerous invasion. Moreover, one of the hypothesized key mediators for the promoted migration, CXCL12, was confirmed using an assay of antagonist LIT‐927. This early study demonstrated that a designed tissue engineering scaffold can be utilized to create a bone‐mimicking environment that serves as a novel platform to recapitulate the pre‐metastatic niche and help interrogate the scheme of bone metastasis by breast cancer.
Structural, biocompatibility, and antibacterial properties of Ge–DLC nanocomposite for biomedical applications
Authors: Arslan Mehmet Enes , Kurt Mustafa Şükrü , Aslan Naim , Kadi Abdurrahim , Öner Sena , Çobanoğlu Şeymanur , Yazici Ayşenur 
Published: February 2022

AbstractIntegrative production of new nanocomposites has been used to enhance favorable features of biomaterials for unlocking ultimate potential of different molecules. In the present study, advantageous properties of diamond like carbons (DLC) and germanium (Ge) like greater biocompatibility and antibacterial attributes were aimed to combined into a thin film. For this purpose, 400 nm DLC–Ge nanocomposite was coated on the borosilicate glasses via the magnetron sputtering and surface characteristics was analyzed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and The Raman spectrum. Biocompatibility analysis were performed by 3‐(4,5‐Dimethylthiazol‐2‐yl) (MTT) cell viability assay and Hoechst 33258 fluorescent staining genotoxicity assessments on the human fibroblast cell line (HDFa). Finally, antibacterial properties of DLC–Ge nanocomposite coatings were investigated by Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923) bacterial attachment analysis. As a result of magnetron sputtering coating, nearly 400 nm thick DLC–Ge nanocomposite film showed a smooth, a non‐porous, and a dense characteristic. Cell viability analysis showed that Ge–DLC coatings permits %95 cell surface growth of fibroblast cells. Also, there were no significant difference in aspect of nuclear abnormalities compared to the (−) control which showed nonmutagenic features of the thin film. Finally, antibacterial attachment analysis put forth that Ge–DLC coatings inhibits bacterial adhesion as %40 and %25 rates for P. aeruginosa and S. aureus bacterial strains, respectively. From these results, DLC–Ge nanocomposites could be proposed as a potential new biomaterial for various biomedical applications.
UK clinical and community psychology: Exploring personal and professional connections
Authors: Thompson Miles , Stuart Jenny , Vincent Rose E. , Goodbody Louise 
Published: February 2022

AbstractThis paper explores the personal and professional connections between clinical psychologists in the United Kingdom (UK) and critical/community psychology (CCP). Specifically, it asks how clinical psychologists define the area, how they relate to it and how they apply it in their work. Twenty clinical psychologists responded to an online survey, 12 of whom went on to take part in a follow‐up telephone interview. Data were analysed using inductive thematic analysis. The results are divided into three sections: i. “describing CCP”: social justice and a questioning stance are considered, ii. “relating to CCP”: an interplay between lifespan events and personal responses are described and iii. “applying CCP”: a dynamic between role‐specific applications and reality checks that either enable or constrain is illustrated. Although the continued need for a CCP is described, the results highlight both challenges and tensions of practising CCP within clinical psychology.
Academic performance and attitudes of dental students impacted by COVID‐19
Authors: Hayes Christina , Mears Michael , Rowan Sean , Dong Fanglong , Andrews Elizabeth 
Published: February 2022

AbstractObjectivesPrevious studies only focused on attitudes and behaviors of US dental students without examining direct effects of the COVID‐19 pandemic on academic performance. This study examined effects of COVID‐19 pandemic on dental students’ academic performance, self‐reported attitudes, behavior, and service utilization. We hypothesized that the pandemic provided more beneficial learning environments.MethodsThis mixed study design implemented a cross sectional survey with retrospective extraction of students’ academic grades. A survey of 274 predoctoral students assessed self‐reported attitudes/behaviors and service utilization. First year Doctor of Dental Medicine (DMD1) 2021–2024 students’ academic performance data were extracted. Independent t‐test and chi‐square crosstab analyses were conducted assessing differences between pre‐COVID and post‐COVID cohorts.ResultsParticipants’ academic grades identified statistically significant associations between pre‐/post‐COVID grades in five of 12 DMD1 courses. Grade percentages identified increased average grades in four of 12 DMD1 courses, with one of 12 courses demonstrating decreased grade percentage. Half of survey participants were female (n = 37/72, 51.4%), 79.2% were 25–34 years old, and 44.4% (n = 32) were DMD 2024. About 1/5 (20.8%, n = 15) sought counseling/therapy. Students agreed staying home allowed more time to study (66.7%, n = 48), while 59.2% (n = 42) reported increased financial concerns. A majority reported lacking in‐person group studying decreased performance, and 55.6% (n = 40) reported feeling depressed.ConclusionStudents performed better overall in courses delivered remotely with clinical application and team‐based engagement. Students performed equally overall; however, the majority had concerns regarding finances, group studying, and mental health challenges. This highlights the need for more readily available resources at institutions.
K‐29 linked ubiquitination of Arrdc4 regulates its function in extracellular vesicle biogenesis
Authors: Farooq Ammara Usman , Gembus Kelly , Sandow Jarrod J. , Webb Andrew , Mathivanan Suresh , Manning Jantina A. , Shah Sonia S. , Foot Natalie J. , Kumar Sharad 
Published: February 2022

AbstractExtracellular vesicles (EVs) are important mediators of intercellular communication. However, EV biogenesis remains poorly understood. We previously defined a role for Arrdc4 (Arrestin domain containing protein 4), an adaptor for Nedd4 family ubiquitin ligases, in the biogenesis of EVs. Here we report that ubiquitination of Arrdc4 is critical for its role in EV secretion. We identified five potential ubiquitinated lysine residues in Arrdc4 using mass spectrometry. By analysing Arrdc4 lysine mutants we discovered that lysine 270 (K270) is critical for Arrdc4 function in EV biogenesis. Arrdc4K270R mutation caused a decrease in the number of EVs released by cells compared to Arrdc4WT, and a reduction in trafficking of divalent metal transporter (DMT1) into EVs. Furthermore, we also observed a decrease in DMT1 activity and an increase in its intracellular degradation in the presence of Arrdc4K270R. K270 was found to be ubiquitinated with K‐29 polyubiquitin chains by the ubiquitin ligase Nedd4‐2. Thus, our results uncover a novel role of K‐29 polyubiquitin chains in Arrdc4‐mediated EV biogenesis and protein trafficking.
The impact of child migration on the health and well‐being of parents left behind
Authors: Bridges Sarah , Liu Lefan 
Published: February 2022

AbstractThis paper uses data from the China Health and Retirement Survey to estimate the causal effect child out‐migration has on the health of parents in China. For rural parents, we show that after controlling for self‐selection, child out‐migration has a detrimental effect not only on subjective well‐being and mental health but also on cognitive function and physical health when measured using a series of clinical tests and health biomarkers. In contrast, for urban parents, only a few health effects are significant, and those that are tend to be positive. In terms of the mechanism through which migration affects parental health, we find that it is differences in the level of physical support that parents require that is central to explaining many of the health effects we observe. For those who require little physical support, the income effect helps to mitigate the negative effects associated with child out‐migration, while for more vulnerable groups of parents, the economic benefits of migration are currently no substitute for the loss of informal support networks when a child migrates.
Historical droughts in Irish catchments 1767–2016
Authors: O'Connor Paul , Murphy Conor , Matthews Tom , Wilby Robert L. 
Published: February 2022

AbstractRecent prolonged dry periods in summer 2018 and spring 2020 have reawakened interest in drought in Ireland, prompting questions regarding historical drought occurrence and potential long‐term risks. Employing 250 years of monthly precipitation and flow reconstructions, we investigate historical drought in Irish catchments evaluating the characteristics (number of events, duration, and deficits) of moderate, severe, and extreme droughts as well as the propagation of meteorological to hydrological drought. Using standardized indices, we identify three distinct catchment types. Cluster 1 catchments, located in the wetter northwest are characterized by small areas, low groundwater storage, and the highest frequency of hydrological drought relative to other catchments. Cluster 3 catchments, located in the drier east and southeast have larger areas, greater groundwater storage, the highest frequency of meteorological drought but the least hydrological droughts. However, once established, droughts in Cluster 3 tend to be more persistent with large accumulated deficits. Cluster 2 catchments, located in the southwest and west, are intermediate to Clusters 1 and 3, with hydrological droughts typically of shorter durations, reduced accumulated deficits but greater mean deficits. The most extreme droughts based on accumulated deficits across all catchments occurred in 1803–1806, 1854–1859, 1933–1935, 1944–1945, 1953–1954, and 1975–1977. Although not as severe, droughts in 1887–1888, 1891–1894, and 1971–1974 also appear as significant extremes. Changes in drought characteristics reveal a complex picture with the direction, magnitude, and significance of trends dependent on the accumulation period used to define drought, the period of record analysed, and the reference period used to standardize indices. Of particular note is a tendency towards shorter, more intense meteorological and hydrological droughts. Our findings offer important insight for drought and water management in Ireland given the paucity of extreme droughts in short observed river flow records.
Preparation, tribological behaviour and lubrication performances of nano‐CaWO4:Eu3+ as EP additive in water‐soluble fluid for laminated copper cladding steel sheet during cold rolling
Authors: Xiong Sang , Wu Hao , Liang Dong 
Published: February 2022

AbstractCaWO4: Eu3+ nanoparticles in acidity (pH = 5) and alkalinity (pH = 10) solutions were prepared by citric acid method, and characterised by SEM and XRD. Tribological behaviour and lubrication performances of nano‐CaWO4:Eu3+ as extreme pressure (EP) additives in water‐soluble fluid for laminated copper cladding steel sheet during cold rolling were studied. These results suggest that CaWO4:Eu3+ exhibits excellent extreme pressure property, antiwear and antifriction performances in water‐soluble fluid, especially when the optimal concentration was 0.3 wt%. The value of WSD reduced from 0.62 to 0.40 mm and the value of COF decreased from 0.078 to 0.068, the surface quality of rolled sheet is improved with the addition of nano‐CaWO4:Eu3+ (pH = 5 and pH = 10), because the tribofilm (~20 nm) composed of Fe2O3, WO3, Eu2O and CaO was formed on the worn surface to avoid direct contact between metal friction pairs in sliding process.
Report of rare and novel mutations in candidate genes in a cohort of hearing‐impaired patients
Authors: Liu Min , Liang Yue , Huang Bixue , Sun Jincangjian , Chen Kaitian 
Published: February 2022

AbstractBackgroundMany hearing‐impaired patients carry mutations in rare or novel genes undetected in regular genetic hot regions/genes screening.MethodsWe collected clinical and genetic data from subjects with hearing loss who visited our department for genetic counseling. Next‐generation sequencing was conducted after 154 deafness‐related genes were captured using a designed genes panels in 14 unrelated families (37 participants). The results were filtered and assessed with in silico tools, in combination with pedigree mapping.ResultsTen mutations in regular deafness genes (GJB2, SLC26A4) and uncommon genes (OTOF, MYO7A, MYO15A, and KARS) were detected, which constituted 57.2% of yielded rate. In particular, two patients with nonsyndromic deafness carried biallelic KARS mutations. In addition, we identified an unreported digenic mutational inheritance in GRP98/USH2A genes in a proband with isolated hearing loss. Functional analyses and molecular modeling suggested the damaging consequence of these variants on encoded proteins. According to the variant pathogenicity guidelines, the 17 identified variants in total were classified as “pathogenic” or “likely pathogenic.”ConclusionThe candidate mutations in deafness genes were suggested to be co‐segregated in at least 57.2% of the studied pedigrees. This is the new report of rare/novel mutations causing inherited hearing loss in Chinese.
Metal‐free Kinugasa reaction catalyzed by external electric field
Authors: Mandal Sucharita , Datta Ayan 
Published: February 2022

AbstractOriented external electric field (OEEF) is in recent interest for its ability to promote reactivity and selectivity in chemical transformations. This article reports facile metal‐free [3 + 2] cycloaddition for the Kinugasa reaction in the presence of OEEF. The free energy of activation is reduced by 4.9 kcal/mol due to a parallel electric field in the orthogonal direction to the reaction axis, Fy = 5.0 V Å−1. As a proof‐of‐principle of this model, a multidrug‐resistant (MDR) antibiotic, carbapenem could be envisioned from the [3 + 2] cycloaddition of a cyclic nitrone and an acetonide substituted acetylene. This reaction shows an interesting ambiaxial electric field‐induced catalysis with a reduced reaction barrier for anti‐parallel fields, −Fx (reaction axis) and −Fz and parallel field along +Fy. This is due to the out‐of‐plane bending of the acetonide unit containing two highly electronegative oxygen atoms. As a result, off‐axial tensorial responses to the electric‐fields on the barriers are observed. Such ambiaxial OEEF driven catalysis is interesting because it expands the utility of electric fields for large enzymes. Disorder and dynamical effects of side chains are envisioned to cause non‐null responses to applied field for parallel or anti‐parallel directions along axes.
Role of cardiopulmonary exercise test in the prediction of hemodynamic impairment in patients with pulmonary arterial hypertension
Authors: Pezzuto B. , Badagliacca R. , Muratori M. , Farina S. , Bussotti M. , Correale M. , Bonomi A. , Vignati C. , Sciomer S. , Papa S. , Palazzo Adriano E. , Agostoni P. 
Published: February 2022

AbstractPeriodic repetition of right heart catheterization (RHC) in pulmonary arterial hypertension (PAH) can be challenging. We evaluated the correlation between RHC and cardiopulmonary exercise test (CPET) aiming at CPET use as a potential noninvasive tool for hemodynamic burden evaluation. One hundred and forty‐four retrospective PAH patients who had performed CPET and RHC within 2 months were enrolled. The following analyses were performed: (a) CPET parameters in hemodynamic variables tertiles; (b) position of hemodynamic parameters in the peak end‐tidal carbon dioxide pressure (PETCO2) versus ventilation/carbon dioxide output (VE/VCO2) slope scatterplot, which is a specific hallmark of exercise respiratory abnormalities in PAH; (c) association between CPET and a hemodynamic burden score developed including mean pulmonary arterial pressure (mPAP), pulmonary vascular resistance (PVR), cardiac index, and right atrial pressure. VE/VCO2 slope and peak PETCO2 significantly varied in mPAP and PVR tertiles, while peak oxygen uptake (peak VO2) and O2 pulse varied in the tertiles of all hemodynamic parameters. PETCO2 versus VE/VCO2 slope showed a strong hyperbolic relationship (R2 = 0.7627). Patients with peak PETCO2 > median (26 mmHg) and VE/VCO2 slope < median (44) presented lower mPAP and PVR (p < 0.005) than patients with peak PETCO2 < median and VE/VCO2 slope > median. Multivariate analysis individuated peak VO2 (p = 0.0158) and peak PETCO2 (p = 0.0089) as hemodynamic score independent predictors; the formula 11.584 − 0.0925 × peak VO2 − 0.0811 × peak PETCO2 best predicts the hemodynamic score value from CPET data. A significant correlation was found between estimated and calculated scores (p < 0.0001), with a precise match for patients with mild‐to‐moderate hemodynamic burden (76% of cases). The results of the present study suggest that CPET could allow to estimate the hemodynamic burden in PAH patients.