bluuebunny/crossref_metadata_2025_split huggingface dataset:
Continuous integrated manufacturing for biopharmaceuticals: A new paradigm or an empty promise?
Authors: Rathore Anurag S. , Thakur Garima , Kateja Nikhil 
Published: September 2022

AbstractContinuous integrated bioprocessing has elicited considerable interest from the biopharma industry for the many purported benefits it promises. Today many major biopharma manufacturers around the world are engaged in the development of continuous process platforms for their products. In spite of great potential, the path toward continuous integrated bioprocessing remains unclear for the biologics industry due to legacy infrastructure, process integration challenges, vague regulatory guidelines, and a diverging focus toward novel therapies. In this article, we present a review and perspective on this topic. We explore the status of the implementation of continuous integrated bioprocessing among biopharmaceutical manufacturers. We also present some of the key hurdles that manufacturers are likely to face during this implementation. Finally, we hypothesize that the real impact of continuous manufacturing is likely to come when the cost of manufacturing is a substantial portion of the cost of product development, such as in the case of biosimilar manufacturing and emerging economies.
Synthesis and Anti‐Fungal/Oomycete Activity of Novel Sulfonamide Derivatives Containing Camphor Scaffold
Authors: Yang Zihui , Sun Xuebao , Jin Daojun , Qiu Yigui , Chen Linlin , Sun Lu , Gu Wen 
Published: September 2022

AbstractTwelve novel camphor sulfonamide derivatives 2a–2l were synthesized and characterized by 1H‐NMR, 13C‐NMR and HRMS spectra. The anti‐fungal/oomycete activity bioassay showed that some of the title compounds displayed moderate to good anti‐fungal/oomycete activities against B. dothidea and P. capsici. Compound 2d exhibited the best in vitro antifungal activity toward B. dothidea. The in vivo experiment revealed that compound 2d possessed considerable anti‐B. dothidea effect at 200 mg/L. Mechanism study showed that compound 2d could increase the cell membrane permeability. In addition, the in vitro enzyme inhibition assay and molecular docking results indicated that compound 2d could be a potential SDH inhibitor.
HSP90 drives the Rab11a‐mediated vesicular transport of the cell surface receptors in osteoclasts
Authors: Tran Manh Tien , Okusha Yuka , Htike Kaung , Sogawa Chiharu , Eguchi Takanori , Kadowaki Tomoko , Sakai Eiko , Tsukuba Takayuki , Okamoto Kuniaki 
Published: September 2022

AbstractRab11a, which ubiquitously localizes to early and recycling endosomes, is required for regulating the vesicular transport of cellular cargos. Interestingly, our previous study revealed that Rab11a served as a negative regulator of osteoclastogenesis by facilitating the lysosomal proteolysis of (1) colony‐stimulating factor‐1 (c‐fms) receptor and (2) receptor activator of nuclear factor‐κB (RANK) receptor, thereby resulting in inhibition of osteoclast (OC) differentiation, maturation, and bone‐resorbing activity. However, the molecular mechanisms of how Rab11a negatively affected osteoclastogenesis were largely unknown. Heat shock protein (HSP90), including two isoforms HSP90α and HSP90β, necessitates the stability, maturation, and activity of a broad range of its clients, and is essentially required for a vast array of signal transduction pathways in nonstressful conditions. Furthermore, cumulative evidence suggests that HSP90 is a vital element of the vesicular transport network. Indeed, our recent study revealed that HSP90, a novel effector protein of Rab11b, modulated Rab11b‐mediated osteoclastogenesis. In this study, we also found that Rab11a interacted with both HSP90α and HSP90β in OCs. Upon blockade of HSP90 ATPase activity by a specific inhibitor(17‐allylamino‐demethoxygeldanamycin), we showed that (1) the ATPase domain of HSP90 was a prerequisite for the interaction between HSP90 and Rab11a, and (2) the interaction of HSP90 to Rab11a sufficiently maintained the inhibitory effects of Rab11a on osteoclastogenesis. Altogether, our findings undoubtedly indicate a novel role of HSP90 in regulating Rab11a‐mediated osteoclastogenesis.
An overview of long noncoding RNAs: Biology, functions, therapeutics, analysis methods, and bioinformatics tools
Authors: Jafari‐Raddani Farideh , Davoodi‐Moghaddam Zeinab , Yousefi Amir‐Mohammad , Ghaffari Seyed H. , Bashash Davood 
Published: September 2022

AbstractLong noncoding RNAs (lncRNAs) are a diverse class of RNAs whose functions are widespread in all branches of life and have been the focus of attention in the last decade. While a huge number of lncRNAs have been identified, there is still much work to be done and plenty to be learned. In the current review, we begin with the biogenesis and function of lncRNAs as they are involved in the different cellular processes from regulating the architecture of chromosomes to controlling translation and post‐translation modifications. Questions on how overexpression, mutations, or deficiency of lncRNAs can affect the cellular status and result in the pathogenesis of various human diseases are responded to. Besides, we allocate an overview of several studies, concerning the application of lncRNAs either as diagnostic and prognostic biomarkers or novel therapeutics. We also introduce the currently available techniques to explore details of lncRNAs such as their function, cellular localization, and structure. In the last section, as exponentially growing data in this area need to be gathered and organized in comprehensive databases, we have a particular focus on presenting general and specialized databases. Taken together, with this review, we aim to provide the latest information on different aspects of lncRNAs to highlight their importance in physiopathologic states and take a step towards helping future studies.
N‐Acyl Amides from Neisseria meningitidis and Their Role in Sphingosine Receptor Signaling
Authors: Cho Wooyoung , York Autumn G. , Wang Rurun , Wyche Thomas P. , Piizzi Grazia , Flavell Richard A. , Crawford Jason M. 
Published: September 2022

AbstractNeisseria meningitidis is a Gram‐negative opportunistic pathogen that is responsible for causing human diseases with high mortality, such as septicemia and meningitis. The molecular mechanisms N. meningitidis employ to manipulate the immune system, translocate the mucosal and blood‐brain barriers, and exert virulence are largely unknown. Human‐associated bacteria encode a variety of bioactive small molecules with growing evidence for N‐acyl amides as being important signaling molecules. However, only a small fraction of these metabolites has been identified from the human microbiota thus far. Here, we heterologously expressed an N‐acyltransferase encoded in the obligate human pathogen N. meningitidis and identified 30 N‐acyl amides with representative members serving as agonists of the G‐protein coupled receptor (GPCR) S1PR4. During this process, we also characterized two mammalian N‐acyl amides derived from the bovine medium. Both groups of metabolites suppress anti‐inflammatory interleukin‐10 signaling in human macrophage cell types, but they also suppress the pro‐inflammatory interleukin‐17A+ population in TH17‐differentiated CD4+ T cells.
Intussusception of the bowel in a young woman: A case report
Authors: Hejazi Peyman , Yousefi Saeed , Hemmati Hossein , Faraji Niloofar , Mohammadyari Fatemeh 
Published: September 2022

AbstractIntussusception is a condition in which a segment of the gastrointestinal tract invaginates into the lumen of another segment. Adult intussusception is less common than juvenile intussusception in terms of cause, appearance, and treatment. Because the clinical picture can be quite atypical and difficult to interpret, it is frequently misdiagnosed at first. Herein, we report the case of a previously healthy 23‐year‐old female patient who presented to the Emergency Department (ED) with acute abdominal pain, vomiting, and diarrhea for 1 day following her last menstrual period. Ileocecal intussusception was discovered throughout the investigation. She was rushed for open abdominal surgery. Meckel's diverticulum was found as a pathologic lead point in the resected specimen, with no evidence of malignancy. Although intussusception is rare in adults, it should be considered in patients who have nonspecific stomach pain.
Fabrication of Hydroxyl‐Carboxyl Bifunctional Hyper‐Crosslinked Polymers for Selective Adsorption of Methylene Blue
Authors: Li Zhiyue , Liu Xuerui , Zhang Xiao , Zhang Taoyi , Chen Jing , Li Quan , Yu Yingchun , Zhao Xiaoyan , Wei Yun 
Published: September 2022

AbstractDyes are usual contaminants in aqueous solution. Some of them are carcinogenic and toxic. Hydroxyl‐carboxyl bifunctional hyper‐crosslinked polymers (HCHCP) carrying hydroxyl and carboxyl groups on their surface were fabricated for methylene blue (MB) adsorption by Friedel‐Crafts alkylation, using 3,4‐dihydroxyphenylacetic acid as a monomer. The maximum adsorption amount of MB was 751.88 mg g−1 according to the Langmuir isotherm model. The adsorption kinetics results demonstrated that the adsorption of MB followed the pseudo‐second‐order kinetic mode. In addition, the results of selective adsorption displayed that the HCHCP could separate MB from Acid Red 91 and methyl orange. Thus, HCHCP are promising adsorbents for cationic dyes.
Near‐Infrared Electrochromic Behavior of Dibenzothiepin Derivatives Attached with Two Michler's Hydrol Blue Units
Authors: Ishigaki Yusuke , Takata Masaki , Shimajiri Takuya , Wu Luyan , Zeng Wenhui , Ye Deju , Suzuki Takanori 
Published: September 2022

Abstract10,11‐Bis[bis(4‐dimethylaminophenyl)methylene]dibenzo[bf]thiepin (1) and ‐oxepin (2) were prepared as stable yellow crystalline compounds, which are the cyclic analogues of electron‐donating hexaarylbutadienes. Upon two‐electron oxidation, they are reversibly transformed into the title dications (12+ and 22+) exhibiting near‐infrared (NIR) absorptions, which were also isolated as stable salts. These redox pairs can serve as new entries into less well‐explored organic NIR‐electrochromic systems, and the separation of redox peaks (electrochemical bistability) was attained for 1/12+ and 2/22+, thanks to drastic geometrical changes between neutral and dicationic states, as revealed by a series of X‐ray analyses. Thiepin‐S,S‐dioxide analogue (3/32+) exhibits quite similar dynamic redox behavior due to nonaromatic nature of the dibenzothiepin and ‐oxepin unit in 12+ and 22+, whereas the thiepin‐S‐oxide derivative (4/42+) does not exhibit bistability due to the smaller change in geometry upon electron transfer, showing that a subtle change of a bridging atom in the central seven‐membered ring can modify the redox properties.
Integration of Microalgae‐Based Wastewater Bioremediation–Biorefinery Process to Promote Circular Bioeconomy and Sustainability: A Review
Authors: Singh Anamika , Shourie Abhilasha , Mazahar Samina 
Published: September 2022

AbstractBioremediation of wastewater using microalgae is inexpensive, energy efficient, and effective in pollutant reduction as compared to conventional wastewater treatment technologies. Wastewater is a huge resource of minerals, nutrients, bioenergy, and valuable organic compounds and can be used for cultivation of microalgae. The microalgal biomass can be further used as biorefinery feedstock to produce biofuels and commercially important high‐value products. The potential of microalgae toward bioremediation and biorefinery applications presents the avenues for integrating the two processes to support circular bioeconomy and sustainability. This review presents a holistic view of integration of bioremediation and biorefinery processes using microalgae for deriving multiple benefits like pollutant removal, resource recovery, biofuel production, and generation of high‐value commercial products. The current status of high‐throughput microalgal screening technologies is also discussed since the selection of suitable microalgal strains is crucial for the application. The review further summarizes various processes involved in bioremediation and biorefinery systems such as cultivation, bioremediation, harvesting, and downstream processing. Recent trends in microalgal strain improvement for bioremediation and biorefinery applications through genetic engineering, bioinformatics, omics technologies, and genome editing tools are highlighted, while addressing the risks, biosafety issues, and regulatory affairs associated with genetically modified algae.
Ultrasound‐Assisted Soil Washing for Metals‐Contaminated Soil Using Various Washing Solutions
Authors: He Liwei , Zhou Xuejin , Cao Jingjing , Shen Liang 
Published: September 2022

AbstractBench‐scale soil washing experiments with and without ultrasound‐assistance are conducted to remove potentially toxic metals (PTMs) from contaminated soils by using artificially contaminated soil samples. Three washing solutions including distilled water, oxalic acid, and citric acid are tested for their efficacy in the removal of PTMs. Ultrasonication time and concentration of the extracting agents are considered as factors affecting the removal efficiency while the mechanism of removal is explored by studying the chemical species of PTMs before and after washing. The results indicate that ultrasound could have significant enhancement on the efficiency of soil washing. Moreover, analysis of the metallic species shows that the removal efficiency is dependent on the type of species. In general, more obvious enhancement of the removal efficiency is detected in the carbonate‐bound and Fe/Mn oxide‐bound fractions of lead, whereas for cadmium, it is for the carbonate‐bound, Fe/Mn oxide‐bound, and organic‐bound fractions compared to four different other forms of the metallic elements.
Associations between geography, decision‐making style, and interest in cancer clinical trial participation
Authors: Caston Nicole E. , Williams Courtney P. , Wan Clara , Ye Star , Pywell Cameron , Ingram Stacey A. , Azuero Andres , Sussell Jesse , Patel Shilpen , Arend Rebecca , Rocque Gabrielle B. 
Published: September 2022

BackgroundClinical trials offer novel treatments, which are essential to high quality cancer care. Patients living in rural areas are often underrepresented in clinical trials due to several factors. This study evaluated the association between rurality and interest in clinical trial participation, change in interest, and treatment decision‐making style preference.MethodsThis cohort study included patients with cancer receiving oncology care at the University of Alabama at Birmingham from 2017 to 2019. Associations between treatment decision‐making preference and the interaction between rurality and area deprivation were analyzed using multinomial logistic regression. Initial interest in clinical trial participation and change in interest were analyzed using modified Poisson regressions with robust standard errors. Initial interest model was stratified by Area Deprivation Index (ADI; higher vs. lower disadvantaged).ResultsIn adjusted models, patients in rural versus urban areas had similar initial interest in clinical trials, both those in higher (40% vs. 50%) and lower disadvantaged settings (54% vs. 62%). Additionally, rural versus urban patients had similar change of clinical trial interest for both those who changed from uninterested‐to‐interested (31% vs. 26%) and interested‐to‐uninterested (47% vs. 42%).ConclusionThis study compares the interest in clinical trial participation among patients living in rural and urban settings. Lack of interest may be secondary to barriers that patients in rural areas face (e.g., transportation, financial, access). Most rural patients prefer a shared treatment decision‐making style, which should be considered when identifying interventions to increase enrollment of underserved rural patients in clinical trials.
Fabrication of DNA‐based Biosensors Driven by Electrostatic Attractions on Electrodes Modified with Reduced Graphene Oxide and Multi‐walled Carbon Nanotubes
Authors: Kumari Prity , Adeloju Samuel 
Published: September 2022

AbstractThe use of reduced graphene oxide (rGO) and multi‐walled carbon nanotubes (MWCNTs) has attracted considerable interest for fabrication of chemical sensors and biosensors in recent years. In this study, we report on two ultrasensitive DNA biosensors based on a sensing mechanism driven by the electrostatic attraction of [Ru(NH3)6]3+ cations on electrode surfaces modified with Au‐MWCNTs and Au‐rGO. A one‐step pot synthesis was used to prepare these nanocomposites for the development of the DNA‐based biosensors. The detection of DNA with the biosensors was based on the electrostatic attraction of [Ru(NH3)6]3+ cations by the anionic phosphate backbone of DNA. The interfacial changes on the electrode surface upon hybridisation were detected by differential pulse voltammetry (DPV). The high conductivity and high surface to volume ratio properties of the carbon materials enabled significant enhancement of the response for the target DNA detection. The DPV response gave linear concentration ranges (LCRs) of 0.1–10,000 pM and 1–100 pM with the Au‐MWCNT and Au‐rGO‐based biosensors, respectively. Thus, indicating that the MWCNT‐based biosensor was more sensitive for DNA detection and achieved a wider LCR. Nevertheless, both MWCNT and rGO‐based biosensors exhibited very good discrimination against mismatch sequences.
Chloride Binding Modulated by Anion Receptors Bearing Tetrazine and Urea
Authors: Plais Romain , Gouarin Guy , Bournier Amélie , Zayene Olfa , Mussard Vanessa , Bourdreux Flavien , Marrot Jérome , Brosseau Arnaud , Gaucher Anne , Clavier Gilles , Salpin Jean‐Yves , Prim Damien 
Published: September 2022

AbstractModulation and fine‐tuning of the strength of weak interactions to bind anions are described in a series of synthetic receptors. The general design of the receptors includes both a urea motif and a tetrazine motif. The synthetic sequence towards three receptors is detailed. Impacts of H‐bond strength and linker length between urea and tetrazine on chloride complexation are studied. Binding properties of the chloride anion are examined in both the ground and excited states using a panel of analytical methods (NMR spectroscopy, mass spectrometry, UV/Visible spectroscopies, and fluorescence). A ranking of the receptors by complexation strength has been determined, allowing a better understanding of the structure‐properties relationship on these compounds.
Ultra‐Bright Near‐Infrared Sulfonate‐Indolizine Cyanine‐ and Squaraine‐Albumin Chaperones: Record Quantum Yields and Applications
Authors: Meador William E. , Kapusta Karina , Owolabi Iyanuoluwani , Autry Shane A. , Saloni Julia , Kolodziejczyk Wojciech , Hammer Nathan I. , Flynt Alex S. , Hill Glake A. , Delcamp Jared H. 
Published: September 2022

AbstractThe Front Cover shows a single near‐infrared dye being laser‐excited and dimly fluorescing in an aqueous biological environment compared to a near‐infrared dye bound to albumin fluorescing brightly in the same environment. Cover designed by Karina Kapusta. Read the full text of the Research Article at 10.1002/cptc.202200127.
Hydrogen Evolution Electrocatalysis with a Molecular Cobalt Bis(alkylimidazole)methane Complex in DMF: a Critical Activity Analysis
Authors: de Vos Sander D. , Otten Maartje , Wissink Tim , Broere Daniël L. J. , Hensen Emiel J. M. , Klein Gebbink Robertus J. M. 
Published: September 2022

Abstract[Co(HBMIMPh2)2](BF4)2 (1) [HBMIMPh2=bis(1‐methyl‐4,5‐diphenyl‐1H‐imidazol‐2‐yl)methane] was investigated for its electrocatalytic hydrogen evolution performance in DMF using voltammetry and during controlled potential/current electrolysis (CPE/CCE) in a novel in‐line product detection setup. Performances were benchmarked against three reported molecular cobalt hydrogen evolution reaction (HER) electrocatalysts, [Co(dmgBF2)2(solv)2] (2) (dmgBF2=difluoroboryldimethylglyoximato), [Co(TPP)] (3) (TPP=5,10,15,20‐tetraphenylporphyrinato), and [Co(bapbpy)Cl](Cl) (4) [bapbpy=6,6′‐bis‐(2‐aminopyridyl)‐2,2′‐bipyridine], showing distinct performances differences with 1 being the runner up in H2 evolution during CPE and the best catalyst in terms of overpotential and Faradaic efficiency during CCE. After bulk electrolysis, for all of the complexes, a deposit on the glassy carbon electrode was observed, and post‐electrolysis X‐ray photoelectron spectroscopy (XPS) analysis of the deposit formed from 1 demonstrated only a minor cobalt contribution (0.23 %), mainly consisting of Co2+. Rinse tests on the deposits derived from 1 and 2 showed that the initially observed distinct activity was (partly) preserved for the deposits. These observations indicate that the molecular design of the complexes dictates the features of the formed deposit and therewith the observed activity.
Comparison of endoscopic submucosal resection with ligation and endoscopic submucosal dissection for small rectal neuroendocrine tumors: A multicenter retrospective study
Authors: Matsuno Kenshi , Miyamoto Hideaki , Kitada Hideki , Yoshimatsu Shinichi , Tamura Fumio , Sakurai Kouichi , Fukubayashi Kotaro , Shono Takashi , Setoyama Hiroko , Matsuyama Taichi , Suko Shinichiro , Narita Rei , Honda Munenori , Tateyama Masakuni , Naoe Hideaki , Morinaga Jun , Tanaka Yasuhito , Gushima Ryosuke 
Published: September 2022

AbstractObjectivesEndoscopic submucosal resection with band ligation (ESMR‐L) and endoscopic submucosal dissection (ESD) are both standard endoscopic resection methods for rectal neuroendocrine tumors (NETs) <10 mm in size. However, there is no definitive consensus on which is better. Here, we compared the efficacy of ESMR‐L and ESD for small rectal NETs.MethodsThis was a multicenter retrospective cohort study including 205 patients with rectal NETs who underwent ESMR‐L or ESD. Treatment outcomes were compared by univariate analysis, multivariate analysis, and inverse probability treatment weighting (IPTW) using propensity scores. Subgroup analysis evaluated the impact of the endoscopist's experience on the technical outcome.ResultsEighty‐nine patients were treated by ESMR‐L and 116 by ESD. The R0 resection rate was not significantly different between the two (90% vs. 92%, p = 0.73). The procedure time of ESMR‐L was significantly shorter than for ESD (17 min vs. 52 min, p < 0.01) and the hospitalization period was also significantly shorter (3 days vs. 5 days, p < 0.01). These results were confirmed by multivariate analysis and also after IPTW adjustment. The procedure time of ESD was significantly prolonged by a less‐experienced endoscopist (49 min vs. 70 min, p = 0.02), but that of ESMR‐L was not affected (17 min vs. 17 min, p = 0.27).ConclusionsFor small rectal NETs, both ESMR‐L and ESD showed similar high complete resection rates. However, considering the shorter procedure time and shorter hospitalization period, ESMR‐L is the more efficient treatment method, especially for less‐experienced endoscopists.