bluuebunny/crossref_metadata_2025_split huggingface dataset:
#YouAreWhatYouTweetCHHH: Identity and fricative epithesis in French-language tweets
Authors: Dalola Amanda 
Published: July 2022

AbstractPhrase-final fricative epithesis (PFFE), often indicated in informal writing with a final-hor-ch, e.g.beaucoup_h, oui_ch, is a sociophonetic variable of Hexagonal French in which utterance-final vowels give way to intense fricative-like whistles. Production research has found PFFE to be used at equal rates among men and women, and perception research has found that native French speakers perceive it to mark either formal speech or intense affect. This research furthers the special issue’s line of inquiry on French variation in forms of digital media by extending the analysis to a sociophonetic variable with a robust life on Twitter. The study compares the pragmatic value of tweets containing PFFE with previously described values and then examines interactions of gender, hostword phrasal location and lexical frequency on its realization. 96.8% of PFFE occurrences in the 2060-token corpus were classified into the pragmatic categories of Formality and Intense Affect. Results suggest that PFFE has become a salient enough sociophonetic variable that 21st-century French users represent it graphically in their tweets, however, its usage is structurally more permissive than in spoken language, signaling that it has taken on an iconic value in digital spaces.
Écrire son vernaculaire: variation et normes communautaires dans les messages textes en français québécois
Authors: Blondeau Hélène , Tremblay Mireille 
Published: July 2022

RÉSUMÉCet article, qui porte sur les messages textes en français québécois, considère cette pratique langagière comme un lieu privilégié de l’expression du vernaculaire. L’analyse situe la langue des textos dans le débat qui a cours sur la diglossie et la variation en français. S’appuyant sur une analyse de textos tirés du corpus Texto4Science, l’article explique comment cette pratique vernaculaire s’appuie, comme l’oral familier, sur une grammaire qui se distingue de façon systématique du français de référence. De l’examen des données se dégage une grammaire à la fois variable et perméable. Enfin, l’article examine l’émergence d’une scripta vernaculaire et une néographie qui révèlent un effort délibéré de représenter des variantes vernaculaires.
Infrasound From Large Earthquakes Recorded on a Network of Balloons in the Stratosphere
Authors: Garcia Raphael F. , Klotz Adrien , Hertzog Albert , Martin Roland , Gérier Solène , Kassarian Ervan , Bordereau Jérôme , Venel Stéphanie , Mimoun David 
Published: July 2022

AbstractThe ground movements induced by seismic waves create acoustic waves propagating upward in the atmosphere, thus providing a practical solution to perform remote sensing of planetary interiors. However, a terrestrial demonstration of a seismic network based on balloon‐carried pressure sensors has not been provided. Here we present the first detection of seismic infrasound from a large magnitude quake on a balloon network. We demonstrate that quake's properties and planet's internal structure can be probed from balloon‐borne pressure records alone because these are generated by the ground movements at the planet surface below the balloon. Various seismic waves are identified, thus allowing us to infer the quake magnitude and location, as well as planetary internal structure. The mechanical resonances of balloon system are also observed. This study demonstrates the interest of planetary geophysical mission concepts based on seismic remote sensing with balloon platforms, and their interest to complement terrestrial seismic networks.
A Hybrid Optimization Framework for Seismic Full Waveform Inversion
Authors: Zhao Zeyu , Sen Mrinal K. , Denel Bertrand , Sun Dong , Williamson Paul 
Published: July 2022

AbstractA hybrid optimization framework is proposed for full waveform inversion (FWI) problems by incorporating derivative information into the model update rule of a global optimization method called Very Fast Simulated Annealing (VFSA). The proposed optimization framework tackles the local minima issue of non‐linear inverse problems. Additionally, it can converge to the close neighborhood of the global solution from different starting points with an improved convergence speed compared to traditional global optimization methods. Applied to large‐scale FWI problems, the proposed framework greatly relaxes the issue of the dependence of FWI on starting models. Given a proper tuning and sufficient number of iterations, hybrid optimization based FWI can render a good background model, which is a good approximation to the ground truth, even with uninformative prior constraints and poor starting models. The output of hybrid optimization based FWI can be used as the starting model for a subsequent local optimization based FWI run to improve the spatial resolution of the result. The proposed hybrid optimization framework is very general, and can be applied to other linear and non‐linear problems that need optimization loops.
Effect of Tides on the Indonesian Seas Circulation and Their Role on the Volume, Heat and Salt Transports of the Indonesian Throughflow
Authors: Katavouta Anna , Polton Jeff A. , Harle James D. , Holt Jason T. 
Published: July 2022

AbstractThe effect of tides on the Indonesian Throughflow (ITF) is explored in a regional ocean model of South East Asia. Our model simulations, with and without tidal forcing, reveal that tides drive only a modest increase in the ITF volume, heat and salt transports toward the Indian Ocean. However, tides drive large regional changes in these transports through Lombok Strait, Ombai Strait and the Timor Sea, and regulate the partitioning of the ITF amongst them. The effect of tidal mixing on the salinity and temperature profiles within the Indonesian Seas drives a small decrease in the heat and salt transports toward the Indian Ocean in all three exit passages. In contrast, the tidal residual circulation due to the interaction between the tides and the topography and stratification (including the effects of tidal mixing on the circulation) leads to a large decrease in the transports toward the Indian Ocean through the Lombok and Ombai straits, but a large increase through the Timor Sea. Hence, the small net contribution from tides to the ITF's volume, heat and salt transports is due to a compensation between large, but opposing tidal residual transports at the combined Lombok and Ombai straits and in the Timor Sea. Our results indicate that explicit representation of tides, often missing in Earth system models, is necessary to accurately capture the ITF's pathway and so the tracer transport from the Pacific into the Indian Ocean.
Detecting and distinguishing indicators of risk for suicide using clinical records
Authors: Ahmedani Brian K. , Cannella Cara E. , Yeh Hsueh-Han , Westphal Joslyn , Simon Gregory E. , Beck Arne , Rossom Rebecca C. , Lynch Frances L. , Lu Christine Y. , Owen-Smith Ashli A. , Sala-Hamrick Kelsey J. , Frank Cathrine , Akinyemi Esther , Beebani Ganj , Busuito Christopher , Boggs Jennifer M. , Daida Yihe G. , Waring Stephen , Gui Hongsheng , Levin Albert M. 
Published: July 2022

AbstractHealth systems are essential for suicide risk detection. Most efforts target people with mental health (MH) diagnoses, but this only represents half of the people who die by suicide. This study seeks to discover and validate health indicators of suicide death among those with, and without, MH diagnoses. This case-control study used statistical modeling with health record data on diagnoses, procedures, and encounters. The study included 3,195 individuals who died by suicide from 2000 to 2015 and 249,092 randomly selected matched controls, who were age 18+ and affiliated with nine Mental Health Research Network affiliated health systems. Of the 202 indicators studied, 170 (84%) were associated with suicide in the discovery cohort, with 148 (86%) of those in the validation cohort. Malignant cancer diagnoses were risk factors for suicide in those without MH diagnoses, and multiple individual psychiatric-related indicators were unique to the MH subgroup. Protective effects across MH-stratified models included diagnoses of benign neoplasms, respiratory infections, and utilization of reproductive services. MH-stratified latent class models validated five subgroups with distinct patterns of indicators in both those with and without MH. The highest risk groups were characterized via high utilization with multiple healthcare concerns in both groups. The lowest risk groups were characterized as predominantly young, female, and high utilizers of preventive services. Healthcare data include many indicators of suicide risk for those with and without MH diagnoses, which may be used to support the identification and understanding of risk as well as targeting of prevention in health systems.
Impact of Anti-T-lymphocyte globulin dosing on GVHD and Immune reconstitution in matched unrelated myeloablative peripheral blood stem cell transplantation
Authors: Massoud Radwan , Klyuchnikov Evgeny , Gagelmann Nico , Zabelina Tatiana , Wolschke Christine , Ayuk Francis , Fritzsche-Friedland Ulrike , Zander Axel , Kröger Nicolaus 
Published: July 2022

AbstractData on the influence of different Anti-lymphocyte globulin (ATLG) doses on graft versus host disease (GVHD) incidence and immune reconstitution in matched unrelated (MUD) allogeneic Stem cell transplantation (allo-SCT) is limited. This retrospective study conducted at the University Medical-Center Hamburg compares GVHD and Immune reconstitution after myeloablative MUD (HLA 10/10) PBSC allogeneic stem cell transplant between 30 mg/Kg (n = 73) and 60 mg/Kg (n = 216) ATLG. Detailed phenotypes of T, B natural killer (NK), natural killer T (NKT) cells were analyzed by multicolor flow at day 30, 100, and 180 posttransplant. Neutrophil and platelet engraftments were significantly delayed in the 60 mg/kg group with a higher Cumulative incidence of Infections (67% vs 75% p = 0.049) and EBV (21% vs 41% p = 0.049) reactivation at day 100 in this group. In the 30 mg/kg group, we observed a faster reconstitution of naïve-B cells (p < 0.0001) and γδ T cells (p = 0.045) at day+30 and a faster naïve helper T-cell (p = 0.046), NK-cells (p = 0.035), and naïve B-cell reconstitution (p = 0.009) at day+180. There were no significant differences in aGVHD, cGVHD, NRM, RI, PFS, and OS between the groups. The choice of ATLG dose has significant impact on IR but not on GVHD after MUD-allo-SCT. Higher doses are associated with delayed engraftment and increased infections.
Targeting mitochondrial metabolism for precision medicine in cancer
Authors: Sainero-Alcolado Lourdes , Liaño-Pons Judit , Ruiz-Pérez María Victoria , Arsenian-Henriksson Marie 
Published: July 2022

AbstractDuring decades, the research field of cancer metabolism was based on the Warburg effect, described almost one century ago. Lately, the key role of mitochondria in cancer development has been demonstrated. Many mitochondrial pathways including oxidative phosphorylation, fatty acid, glutamine, and one carbon metabolism are altered in tumors, due to mutations in oncogenes and tumor suppressor genes, as well as in metabolic enzymes. This results in metabolic reprogramming that sustains rapid cell proliferation and can lead to an increase in reactive oxygen species used by cancer cells to maintain pro-tumorigenic signaling pathways while avoiding cellular death. The knowledge acquired on the importance of mitochondrial cancer metabolism is now being translated into clinical practice. Detailed genomic, transcriptomic, and metabolomic analysis of tumors are necessary to develop more precise treatments. The successful use of drugs targeting metabolic mitochondrial enzymes has highlighted the potential for their use in precision medicine and many therapeutic candidates are in clinical trials. However, development of efficient personalized drugs has proved challenging and the combination with other strategies such as chemocytotoxic drugs, immunotherapy, and ketogenic or calorie restriction diets is likely necessary to boost their potential. In this review, we summarize the main mitochondrial features, metabolic pathways, and their alterations in different cancer types. We also present an overview of current inhibitors, highlight enzymes that are attractive targets, and discuss challenges with translation of these approaches into clinical practice. The role of mitochondria in cancer is indisputable and presents several attractive targets for both tailored and personalized cancer therapy.
Epigenetic modulators of B cell fate identified through coupled phenotype-transcriptome analysis
Authors: Kong Isabella Y. , Trezise Stephanie , Light Amanda , Todorovski Izabela , Arnau Gisela Mir , Gadipally Sreeja , Yoannidis David , Simpson Kaylene J. , Dong Xueyi , Whitehead Lachlan , Tempany Jessica C. , Farchione Anthony J. , Sheikh Amania A. , Groom Joanna R. , Rogers Kelly L. , Herold Marco J. , Bryant Vanessa L. , Ritchie Matthew E. , Willis Simon N. , Johnstone Ricky W. , Hodgkin Philip D. , Nutt Stephen L. , Vervoort Stephin J. , Hawkins Edwin D. 
Published: July 2022

AbstractHigh-throughput methodologies are the cornerstone of screening approaches to identify novel compounds that regulate immune cell function. To identify novel targeted therapeutics to treat immune disorders and haematological malignancies, there is a need to integrate functional cellular information with the molecular mechanisms that regulate changes in immune cell phenotype. We facilitate this goal by combining quantitative methods for dissecting complex simultaneous cell phenotypic effects with genomic analysis. This combination strategy we term Multiplexed Analysis of Cells sequencing (MAC-seq), a modified version of Digital RNA with perturbation of Genes (DRUGseq). We applied MAC-seq to screen compounds that target the epigenetic machinery of B cells and assess altered humoral immunity by measuring changes in proliferation, survival, differentiation and transcription. This approach revealed that polycomb repressive complex 2 (PRC2) inhibitors promote antibody secreting cell (ASC) differentiation in both murine and human B cells in vitro. This is further validated using T cell-dependent immunization in mice. Functional dissection of downstream effectors of PRC2 using arrayed CRISPR screening uncovered novel regulators of B cell differentiation, includingMybl1,Myof,Gas7andAtoh8. Together, our findings demonstrate that integrated phenotype-transcriptome analyses can be effectively combined with drug screening approaches to uncover the molecular circuitry that drives lymphocyte fate decisions.
Aurora-A/FOXO3A/SKP2 axis promotes tumor progression in clear cell renal cell carcinoma and dual-targeting Aurora-A/SKP2 shows synthetic lethality
Authors: Li Pu , Chen Tingting , Kuang Peng , Liu Fujun , Li Zhongmin , Liu Fangfang , Wang Yu , Zhang Wenfeng , Cai Xiuyu 
Published: July 2022

AbstractRenal cell carcinoma (RCC) is a common malignant tumor in the world. Histologically, most of RCC is classified as clear cell renal cell carcinoma (ccRCC), which is the most prevalent subtype. The overall survival of patients with ccRCC is poor, thus it is urgent to further explore its mechanism and target. S-phase kinase-associated protein 2 (SKP2) is overexpressed in a variety of human cancers and is associated with poor prognosis by enhancing tumor progression. However, it is unclear whether or how SKP2 is involved in ccRCC progression. Here, we reported that overexpression of SKP2 enhanced cell proliferation of ccRCC, while SKP2 depletion exhibited the opposite effect. Bioinformatic analyses found that SKP2 was positively correlated with Aurora-A (Aur-A) in ccRCC. The protein and mRNA levels of SKP2 were elevated or reduced by Aur-A overexpression or silencing, respectively. It was further found that Aur-A caused an increase phosphorylation of FOXO3A, which is a negatively transcription factor for SKP2. Interestingly, SKP2 mediated ubiquitylation and degradation of FOXO3A depend on the kinase activity of Aur-A. The combination of Aur-A inhibitor MLN8237 and SKP2 inhibitor SZL P1-41 showed a synergistic tumor growth inhibition in vivo and in vitro of ccRCC models. Thus, our data reveal that Aurora-A/FOXO3A/SKP2 axis promotes tumor progression in ccRCC, and the double inhibition of SKP2 and Aur-A shows significant synergistic effect, which indicates a potential new therapeutic strategy for ccRCC.
Novel roles of luteinizing hormone (LH) in tissue regeneration-associated functions in endometrial stem cells
Authors: Park Se-Ra , Kim Seong-Kwan , Kim Soo-Rim , Park Jeong-Ran , Lim Soyi , Hong In-Sun 
Published: July 2022

AbstractLuteinizing hormone (LH) stimulates the synthesis and secretion of the key steroid hormone estrogen, which subsequently promotes ovarian follicular growth and development. Therefore, the administration of exogenous LH to achieve superovulation (multiple ovulations) and an LH surge is commonly used as the most effective therapeutic option in a majority of in vitro fertilization (IVF) clinics. However, a relatively low pregnancy rate (between 20% and 35%) is one of the most challenging aspects of LH-based infertility treatment. Furthermore, the major cause of this low pregnancy rate in LH-based infertility treatment remains unidentified. Recent studies have shown that endometrial stem cell loss or deficiency can significantly decrease tissue regeneration ability during the menstrual cycle and reduce endometrial receptivity. In this context, we postulated that the low pregnancy rates following LH-based ovarian hyperactivation may be the result of the adverse effects of consecutive exogenous LH administration on endometrial stem cells. To the best of our knowledge, this study revealed for the first time that in addition to its previously reported roles in stimulating ovarian functions through the pituitary-gonadal axis, LH brings about the extragonadal suppression of various tissue regeneration-associated functions in endometrial stem cells, such as self-renewal, migration ability, multilineage differentiation potential, and pluripotency/stemness, by inhibiting pro-survival Akt and ERK1/2 signaling pathways in vitro and in vivo, and as a consequence, it decreases the endometrial receptivity.
Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC)
Authors: Hui Zhenzhen , Zhang Jiali , Ren Yulin , Li Xiaoling , Yan Cihui , Yu Wenwen , Wang Tao , Xiao Shanshan , Chen Yulong , Zhang Ran , Wei Feng , You Jian , Ren Xiubao 
Published: July 2022

AbstractThe combination of immune checkpoint inhibitors (ICIs) with chemotherapy (chemoimmunotherapy) in the neoadjuvant setting have achieved favorable clinical benefits in non-small cell lung cancer (NSCLC), but the mechanism of clinical responses remain unclear. We provide a rich resource of 186,477 individual immune cells from 48 samples of four treatment-naive and eight neoadjuvant chemoimmunotherapy treated IIIA NSCLC patients (responders versus non-responders) by single-cell RNA-seq and TCR-seq. We observed the synergistic increase of B cells and CD4+ T cells were associated with a positive therapeutic response of neoadjuvant chemoimmunotherapy. B cell IgG subclasses IgG1 and IgG3 played a critical role in anti-tumor immune response in tumor lesions, and this process was driven by increased IL-21 secreted by infiltrated T follicular helper (Tfh) cells after neoadjuvant chemoimmunotherapy. Furthermore, we uncovered several critical events for positive clinical outcomes, including the diminished activated TNFRSF4+ regulatory T cells (Tregs), increased LAMP3+ dendritic cells (DCs), and the expansion of intratumoral CD4+ T clones and peripheral C3-Cytotoxic CD8+ T clones. A validation cohort of 26 treatment-naive and 30 neoadjuvant chemoimmunotherapy treated IIIA/ IIIB NSCLC patients verified these findings. In total, our comprehensive study of the single-cell profile of immune cells provides insights into mechanisms underlying anti-PD-1-based therapies and identified potential predictive factors and therapeutic targets for improving the efficiency of neoadjuvant chemoimmunotherapy in NSCLC.
Freeze-derived heterogeneous structural color films
Authors: Miao Shuangshuang , Wang Yu , Sun Lingyu , Zhao Yuanjin 
Published: July 2022

AbstractStructural colors have a demonstrated value in constructing various functional materials. Efforts in this area are devoted to developing stratagem for generating heterogeneous structurally colored materials with new architectures and functions. Here, inspired by icing process in nature and ice-templating technologies, we present freeze-derived heterogeneous structural color hydrogels with multiscale structural and functional features. We find that the space-occupying effect of ice crystals is helpful for tuning the distance of non-close-packed colloidal crystal nanoparticles, resulting in corresponding reflection wavelength shifts in the icing area. Thus, by effectively controlling the growth of ice crystals and photo-polymerizing them, structural color hydrogels with the desired structures and morphologies can be customized. Other than traditional monochromatic structure color hydrogels, the resultant hydrogels can be imparted with heterogeneous structured multi-compartment body and multi-color with designed patterns through varying the freezing area design. Based on these features, we have also explored the potential value of these heterotypic structural color hydrogels for information encryptions and decryptions by creating spatiotemporally controlled icing areas. We believe that these inverse ice-template structural color hydrogels will offer new routes for the construction and modulation of next generation smart materials with desired complex architectures.
Monomeric prefusion structure of an extremophile gamete fusogen and stepwise formation of the postfusion trimeric state
Authors: Feng Juan , Dong Xianchi , Su Yang , Lu Chafen , Springer Timothy A. 
Published: July 2022

AbstractHere, we study the gamete fusogen HAP2 from Cyanidioschyzon merolae (Cyani), an extremophile red algae that grows at acidic pH at 45 °C. HAP2 has a trimeric postfusion structure with similarity to viral class II fusion proteins, but its prefusion structure has been elusive. The crystal structure of a monomeric prefusion state of Cyani HAP2 shows it is highly extended with three domains in the order D2, D1, and D3. Three hydrophobic fusion loops at the tip of D2 are each required for postfusion state formation. We followed by negative stain electron microscopy steps in the process of detergent micelle-stimulated postfusion state formation. In an intermediate state, two or three linear HAP2 monomers associate at the end of D2 bearing its fusion loops. Subsequently, D2 and D1 line the core of a trimer and D3 folds back over the exterior of D1 and D2. D3 is not required for formation of intermediate or postfusion-like states.