bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exopolysaccharides amylovoran and levan contribute to sliding motility in the fire blight pathogen Erwinia amylovora
Authors: Yuan Xiaochen , Eldred Lauren I. , Sundin George W. 
Published: September 2022

SummaryErwinia amylovora, the causative agent of fire blight, uses flagella‐based motilities to translocate to host plant natural openings; however, little is known about how this bacterium migrates systemically in the apoplast. Here, we reveal a novel surface motility mechanism, defined as sliding, in E. amylovora. Deletion of flagella assembly genes did not affect this movement, whereas deletion of biosynthesis genes for the exopolysaccharides (EPSs) amylovoran and levan resulted in non‐sliding phenotypes. Since EPS production generates osmotic pressure that potentially powers sliding, we validated this mechanism by demonstrating that water potential positively contributes to sliding. In addition, no sliding was observed when the water potential of the surface was lower than −0.5 MPa. Sliding is a passive motility mechanism. We further show that the force of gravity plays a critical role in directing E. amylovora sliding on unconfined surfaces but has a negligible effect when cells are sliding in confined microcapillaries, in which EPS‐dependent osmotic pressure acts as the main force. Although amylovoran and levan are both required for sliding, we demonstrate that they exhibit different roles in bacterial communities. In summary, our study provides fundamental knowledge for a better understanding of mechanisms that drive bacterial sliding motility.
End of consensus? Ideology, partisan identity, and affective polarization in Finland 2003–2019
Authors: Kawecki Daniel 
Published: September 2022

AbstractFinland is traditionally associated with a consensus‐like political culture and low partisan hostility. Despite this, concerns about political polarization have become widespread. A possible explanation is that affective polarization, a phenomenon characterized by increasing emotional distances between political groups, is increasing. This article examines trends and individual‐level predictors of affective polarization in the Finnish electorate between 2003 and 2019, using survey data from five national election studies. Additionally, it contributes to the theoretical understanding of affective polarization in multiparty settings by introducing a measure based on the absence of neutrality towards parties. This measure focuses on individual‐level polarization, thus allowing comparisons between groups of voters. The results show a general increase in affective polarization over the period. Ideological extremity is identified as the main predictor of the phenomenon. Left‐right extremity was the strongest predictor at the beginning of the period, whereas GAL‐TAN extremity became increasingly important over time. After 2011, the effects of ideological extremity became asymmetrical: extreme left and TAN positions predicted higher levels than their ideological opposites. Feeling close to a party had a mostly consistent effect on affective polarization, albeit not as large as ideological extremity. Increasing affective polarization might undermine the prevailing consensus culture in Finnish politics. However, it might also have positive effects as a corrective to voter apathy, leading to increased participation. This makes Finland an interesting case for further studies of the causes, effects, and dynamics of affective polarization.
Comparative transcriptome analysis reveals molecular adaptations underlying distinct immunity and inverted resting posture in bats
Authors: WU Jinwei , ZHANG Libiao , SHEN Chao , SIN Simon Yung Wa , LEI Caoqi , ZHAO Huabin 
Published: September 2022

AbstractUnderstanding how natural selection shapes unique traits in mammals is a central topic in evolutionary biology. The mammalian order Chiroptera (bats) is attractive for biologists as well as the general public due to their specific traits of extraordinary immunity and inverted resting posture. However, genomic resources for bats that occupy key phylogenetic positions are not sufficient, which hinders comprehensive investigation of the molecular mechanisms underpinning the origin of specific traits in bats. Here, we sequenced the transcriptomes of 5 bats that are phylogenetically divergent and occupy key positions in the phylogenetic tree of bats. In combination with the available genomes of 19 bats and 21 other mammals, we built a database consisting of 10 918 one‐to‐one ortholog genes and reconstructed phylogenetic relationships of these mammals. We found that genes related to immunity, bone remodeling, and cardiovascular system are targets of natural selection along the ancestral branch of bats. Further analyses revealed that the T cell receptor signaling pathway involved in immune adaptation is specifically enriched in bats. Moreover, molecular adaptations of bone remodeling, cardiovascular system, and balance sensing may help to explain the reverted resting posture in bats. Our study provides valuable transcriptome resources, enabling us to tentatively identify genetic changes associated with bat‐specific traits. This work is among the first to advance our understanding of the molecular underpinnings of inverted resting posture in bats, which could provide insight into healthcare applications such as hypertension in humans.
Hybrid mixture theory‐based modeling of transport of fluids, species, and heat in food biopolymers subjected to freeze–thaw cycles
Authors: Zhao Ying , Kumar Pavitra Krishna , Sablani Shyam S. , Takhar Pawan S. 
Published: September 2022

AbstractA hybrid mixture theory (HMT)‐based unsaturated transport (pores not saturated with liquid) model was applied to a food matrix subjected to freezing and freeze–thaw cycles. The model can explain the fluid, species, and heat transport, ice formation, thermomechanical changes, and the freezing point depression occurring inside food biopolymers during freezing. Volume changes during freezing were calculated using the stresses due to pore pressure and the phase‐change based mechanical strain. The Eulerian‐Lagrangian transformation was performed for solving the equations using a finite element mesh in Lagrangian coordinates. The predicted temperature profiles for constant and fluctuating freezing temperature conditions showed agreement with experimental data with reasonable accuracy (RMSE = 2.86°C and 2.23°C, respectively). The multiscale transport model coupled with a physical chemistry‐based relation was able to predict solute concentration and the freezing point depression in potatoes with greater accuracy than an empirical equation published in the literature. Sudden temperature fluctuations representing the opening and closing of a freezer door were investigated using this solution scheme, and conditions causing less damage to the food were identified.Practical ApplicationFood materials are subjected to freeze–thaw cycles during storage, shipping, and distribution to the consumers. The study uses numerical modeling and experimental validation to elucidate the principles affecting ice formation, solute migration, and temperature changes. Outcomes will allow processors to improve the quality of frozen foods with improved design of freezing operation, and storage and distribution strategies.
Tenascin C in radiation‐induced lung damage: Pathological expression and serum level elevation
Authors: Toyomasu Yutaka , Matsui Kenta , Omori Kazuki , Takada Akinori , Imanaka‐Yoshida Kyoko , Tawara Isao , Shimamoto Akira , Takao Motoshi , Kobayashi Hiroyasu , Tomaru Atsushi , Fujimoto Hajime , Kobayashi Tetsu , Sakuma Haiime , Nomoto Yoshihito 
Published: September 2022

AbstractRadiation‐induced lung damage (RILD) is a critical problem in lung cancer radiotherapy, and it is difficult to predict its severity. Although no biomarkers for RILD have been established, tenascin C (TNC) is an extracellular matrix glycoprotein involved in the remodeling of damaged tissues and has been implicated in inflammation and fibrosis. We report the unique case of a 36‐year‐old man with adenocarcinoma of the lung, Union for International Cancer Control stage IIIB, who was treated with radiotherapy before lung surgery. The surgical specimen showed histopathological expression of TNC in the region where radiation pneumonitis was observed radiographically. Serum TNC levels were elevated after radiotherapy. In this case, TNC is suggested to be implicated in RILD and may be a potential candidate as a biomarker for the onset and severity of the condition.
New insights into human immune memory from SARS‐CoV‐2 infection and vaccination
Authors: Hartley Gemma E. , Edwards Emily S. J. , O’Hehir Robyn E. , van Zelm Menno C. 
Published: September 2022

AbstractSince early 2020, the world has been embroiled in an ongoing viral pandemic with SARS‐CoV‐2 and emerging variants resulting in mass morbidity and an estimated 6 million deaths globally. The scientific community pivoted rapidly, providing unique and innovative means to identify infected individuals, technologies to evaluate immune responses to infection and vaccination, and new therapeutic strategies to treat infected individuals. Never before has immunology been so critically at the forefront of combatting a global pandemic. It has now become evident that not just antibody responses, but formation and durability of immune memory cells following vaccination are associated with protection against severe disease from SARS‐CoV‐2 infection. Furthermore, the emergence of variants of concern (VoC) highlight the need for immunological markers to quantify the protective capacity of Wuhan‐based vaccines. Thus, harnessing and modulating the immune response is key to successful vaccination and treatment of disease. We here review the latest knowledge about immune memory generation and durability following natural infection and vaccination, and provide insights into the attributes of immune memory that may protect from emerging variants.
Production technology and knowledge transfer of calcite‐tempered grey ware bowls from 2nd‐ to 5th‐century ceNoricum (Austria)
Authors: Borgers Barbara , Ionescu Corina , Gál Ágnes , Neubauer Franz , Von Hagke Christoph , Auer Martin , Szilagyi Veronika , Kasztovszky Zsolt , Gméling Katalin , Harsányi Ildikó , Barbu‐Tudoran Lucian 
Published: September 2022

AbstractAspects of 2nd‐ to 5th‐century ce Roman production technology and knowledge transfer in southern Austria (known as Noricum) were examined. With no evidence for workshops identified in the study area, 44 grey ware bowls from two sites at Aguntum and Lavant were studied macroscopically, and combined with optical microscopy, X‐ray powder diffraction, prompt gamma activation, neutron activation and scanning electron microscopy, in order to understand whether one (large) workshop supplied these bowls, or whether the bowls were produced by several (small) workshops nearby. Combined with information from the geological background, the results were used to tentatively indicate the production location.The results indicate that the grey ware bowls from Aguntum and Lavant were produced by local workshops nearby. The bowls were manufactured with similar clay sources, tempered with crushed calcite‐marble rocks from the Tauern Window, their surface smoothed and burnished, and fired between 800 and 850°C in a reducing atmosphere of an open fire. This is taken to suggest that Roman potters, who were located at Aguntum and Lavant, shared strategies of raw materials selection, paste preparation, finishing and firing, and transferred technological knowledge through time.
Identifying targets for interventions to support public use of face coverings
Authors: Armitage Christopher J. , Keyworth Chris , Gartland Nicola , Coleman Anna , Fishwick David , Johnson Sheena , van Tongeren Martie 
Published: September 2022

AbstractObjectivesInterventions to promote the wearing of face coverings if required in the future can only be developed if we know why people do or do not wear them. Study aims were, therefore, to assess public adherence to wearing face coverings to reduce transmission of SARS‐CoV‐2 and to gauge why people were or were not wearing face coverings in work, public transport, and indoor leisure settings.DesignCross‐sectional survey.Methods10,622 adults (25 January–6 February 2022) who were representative of the UK population were asked about their (a) wearing of face coverings in work, public transport, and leisure settings; (b) sociodemographic characteristics; and (c) perceptions of capabilities, opportunities, and motivations (“COM‐B"). Data were analysed descriptively, using within‐participants ANOVA and multiple linear regression.ResultsParticipants reported mostly wearing face coverings in public transport settings (>80%), but substantially less in work (<50%) and leisure (<30%) contexts. Perceptions of capabilities, opportunities, and motivations to wear face coverings were consistently associated with the actual wearing of face coverings across the three settings, but there were marked deficits in automatic motivation and social opportunity. People living in England, describing themselves as White, and men were least likely to wear face coverings.ConclusionsInterventions targeted at men, people living in England, and those describing themselves as White that focus on increasing capabilities, providing greater opportunities and boosting motivations are suggested to promote the wearing of face coverings, with particular focus on addressing automatic motivation and social opportunity.
Location, extent, and magnitude of dynamic topography in the Late Cretaceous Cordilleran Foreland Basin, USA: New insights from 3D flexural backstripping
Authors: Li Zhiyang , Aschoff Jennifer 
Published: September 2022

AbstractMantle‐induced dynamic topography (i.e., subsidence and uplift) has been increasingly recognized as an important process in foreland basin development. However, characterizing and distinguishing the effects (i.e., location, extent and magnitude) of dynamic topography in ancient foreland basins remains challenging because the spatio‐temporal footprint of dynamic topography and flexural topography (i.e., generated by topographic loading) can overlap. This study employs 3D flexural backstripping of Upper Cretaceous strata in the central part of the North American Cordilleran foreland basin (CFB) to better quantify the effects of dynamic topography. The extensive stratigraphic database and good age control of the CFB permit the regional application of 3D flexural backstripping in this basin for the first time. Dynamic topography started to influence the development of the CFB during the late Turonian to middle Campanian (90.2–80.2 Ma) and became the dominant subsidence mechanism during the middle to late Campanian (80.2–74.6 Ma). The area influenced by >100 m dynamic subsidence is approximately 400 by 500 km, within which significant (>200 m) dynamic subsidence occurs in an irregular‐shaped (i.e., lunate) subregion. The maximum magnitude of dynamic subsidence is 300 ± 100 m based on the 80.2–74.6 Ma tectonic subsidence maps. With the maximum magnitude of dynamic uplift being constrained to be 200–300 m, the gross amount of dynamic topography in the Late Cretaceous CFB is 500–600 m. Although the location of dynamic subsidence revealed by tectonic subsidence maps is generally consistent with isopach map trends, tectonic subsidence maps developed through 3D flexural backstripping provide more accurate constraints of the areal extent, magnitude and rate of dynamic topography (as well as flexural topography) in the CFB through the Late Cretaceous. This improved understanding of dynamic topography in the CFB is critical for refining current geodynamic models of foreland basins and understanding the surface expression of mantle processes.
Development and implementation of fidelity assessment in first episode psychosis services in Czechia: A pilot study
Authors: Tomaskova Hana , Kondrátová Lucie , Winkler Petr , Addington Donald 
Published: September 2022

AbstractAimThe aim of the study was to evaluate fidelity in first episode psychosis (FEP) teams in Czechia and to gage the feasibility and utility of the process in a mental health system that is undergoing a transformation.MethodsFidelity assessment was conducted using The First Episode Psychosis Services Fidelity Scale (FEPS‐FS). Fidelity assessment was based on a review of data abstracted from the health records of active clients, program documents, administrative data, and interviews with members of staff. The mean scores were compared across the teams. Feasibility and utility were assessed by program response to their fidelity results.ResultsThree FEP teams were involved in the fidelity assessment. Across the 35 items, the mean fidelity score ranged from 2.5 to 3.1. Across the FEP teams, the percentage of the 35 items rated as 4 or 5 (satisfactory or exemplary) ranged from 34.3% to 51.4%.ConclusionsThis study provided an opportunity to implement FEPS‐FS and assess fidelity in FEP teams in Czechia. The fidelity assessment also provided a baseline for measuring change.
A globally relevant change taxonomy and evidence‐based change framework for land monitoring
Authors: Lucas Richard M. , German Sophia , Metternicht Graciela , Schmidt Rebecca K. , Owers Christopher J. , Prober Suzanne M. , Richards Anna E. , Tetreault‐Campbell Sally , Williams Kristen J. , Mueller Norman , Tissott Belle , Chua Sean M. T. , Cowood Alison , Hills Terry , Gunawardana Dayani , McIntyre Alexis , Chognard Sebastien , Hurford Clive , Planque Carole , Punalekar Suvarna , Clewley Daniel , Sonnenschein Ruth , Murray Nicholas J. , Manakos Ioannis , Blonda Palma , Owers Kate , Roxburgh Stephen , Kay Heather , Bunting Peter , Horton Claire 
Published: September 2022

AbstractA globally relevant and standardized taxonomy and framework for consistently describing land cover change based on evidence is presented, which makes use of structured land cover taxonomies and is underpinned by the Driver‐Pressure‐State‐Impact‐Response (DPSIR) framework. The Global Change Taxonomy currently lists 246 classes based on the notation ‘impact (pressure)’, with this encompassing the consequence of observed change and associated reason(s), and uses scale‐independent terms that factor in time. Evidence for different impacts is gathered through temporal comparison (e.g., days, decades apart) of land cover classes constructed and described from Environmental Descriptors (EDs; state indicators) with pre‐defined measurement units (e.g., m, %) or categories (e.g., species type). Evidence for pressures, whether abiotic, biotic or human‐influenced, is similarly accumulated, but EDs often differ from those used to determine impacts. Each impact and pressure term is defined separately, allowing flexible combination into ‘impact (pressure)’ categories, and all are listed in an openly accessible glossary to ensure consistent use and common understanding. The taxonomy and framework are globally relevant and can reference EDs quantified on the ground, retrieved/classified remotely (from ground‐based, airborne or spaceborne sensors) or predicted through modelling. By providing capacity to more consistently describe change processes—including land degradation, desertification and ecosystem restoration—the overall framework addresses a wide and diverse range of local to international needs including those relevant to policy, socioeconomics and land management. Actions in response to impacts and pressures and monitoring towards targets are also supported to assist future planning, including impact mitigation actions.
Rebuilding fish biomass for the world's marine ecoregions under climate change
Authors: Cheung William W. L. , Palacios‐Abrantes Juliano , Frölicher Thomas L. , Palomares Maria Lourdes , Clarke Tayler , Lam Vicky W. Y. , Oyinlola Muhammed A. , Pauly Daniel , Reygondeau Gabriel , Sumaila U. Rashid , Teh Lydia C. L. , Wabnitz Colette C. C. 
Published: September 2022

AbstractRebuilding overexploited marine populations is an important step to achieve the United Nations' Sustainable Development Goal 14—Life Below Water. Mitigating major human pressures is required to achieve rebuilding goals. Climate change is one such key pressure, impacting fish and invertebrate populations by changing their biomass and biogeography. Here, combining projection from a dynamic bioclimate envelope model with published estimates of status of exploited populations from a catch‐based analysis, we analyze the effects of different global warming and fishing levels on biomass rebuilding for the exploited species in 226 marine ecoregions of the world. Fifty three percent (121) of the marine ecoregions have significant (at 5% level) relationship between biomass and global warming level. Without climate change and under a target fishing mortality rate relative to the level required for maximum sustainable yield of 0.75, we project biomass rebuilding of 1.7–2.7 times (interquartile range) of current (average 2014–2018) levels across marine ecoregions. When global warming level is at 1.5 and 2.6°C, respectively, such biomass rebuilding drops to 1.4–2.0 and 1.1–1.5 times of current levels, with 10% and 25% of the ecoregions showing no biomass rebuilding, respectively. Marine ecoregions where biomass rebuilding is largely impacted by climate change are in West Africa, the Indo‐Pacific, the central and south Pacific, and the Eastern Tropical Pacific. Coastal communities in these ecoregions are highly dependent on fisheries for livelihoods and nutrition security. Lowering the targeted fishing level and keeping global warming below 1.5°C are projected to enable more climate‐sensitive ecoregions to rebuild biomass. However, our findings also underscore the need to resolve trade‐offs between climate‐resilient biomass rebuilding and the high near‐term demand for seafood to support the well‐being of coastal communities across the tropics.
Functional prognosis in patients with sarcopenic dysphagia: An observational cohort study from the Japanese sarcopenic dysphagia database
Authors: Nagai Takako , Wakabayashi Hidetaka , Nishioka Shinta , Momosaki Ryo 
Published: September 2022

AimIn sarcopenic dysphagia, confirmation of recovery of the swallowing function and activity of daily living is insufficient. The aim of this study was to examine differences in the recovery of the swallowing function and activities of daily living between sarcopenic dysphagia patients and non‐sarcopenic dysphagia patients.MethodsThe registry data of the Japanese Sarcopenic Dysphagia Database were used for the analysis; 440 patients met the eligibility criteria of the study. Dysphagia was evaluated according to the Food Intake Level Scale and a five‐step diagnostic algorithm for the disorder. Patients were divided into two groups according to the presence or absence of sarcopenic dysphagia.ResultsSarcopenic dysphagia was found in 268 cases (60.9%). The rate of improvement in the Food Intake Level Scale was 47.4% in sarcopenic dysphagia patients and 62.2% in non‐sarcopenic dysphagia patients. The rate of improvement in the Food Intake Level Scale was lower in the sarcopenic dysphagia patients (P = 0.008). A multiple regression analysis showed that the presence of sarcopenic dysphagia had a significant influence on changes in the Food Intake Level Scale and Barthel Index (β = −0.191, 95% confidence interval, −1.484 to −0.530, P < 0.001, β = 0.112, 95% confidence interval, 0.587 to 10.450, P = 0.028).ConclusionImprovement in the swallowing function was poor in patients with sarcopenic dysphagia. Sarcopenic dysphagia should be examined to improve swallowing function. Geriatr Gerontol Int 2022; 22: 839–845.