bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of different domains of physical activity with health-related quality of life in adults: 2-year cohort
Authors: Scarabottolo Catarina Covolo , Tebar William Rodrigues , Gobbo Luis Alberto , Ohara David , Ferreira Aline Duarte , da Silva Canhin Daniel , Christofaro Diego Giulliano Destro 
Published: April 2022

Abstract
Background
It is estimated that, worldwide, 9% of deaths occur as a result of insufficient physical activity (PA) practice. Practicing PA can prevent and/or reduce the deleterious effects of different types of diseases and can improve general health aspects related to health-related quality of life (HRQoL).

Purpose
To analyze the relationship of different PA domains with different HRQoL domains over a two-year period.

Methods
This is an observational study with a two-year longitudinal design. The sample, composed of adults, was selected from a randomization of the streets of the different regions (north, south, east, west and center) covering individuals from all areas of the city. To assess the practice of PA, the Baecke questionnaire was used. The instrument Medical Outcomes Study SF-36-Item Short Form Health Survey was used to assess the HRQoL domains. Linear regression models were used to analyzed the association of different PA domains with changes in HRQoL. Multivariate statistical models were adjusted for gender, age, socioeconomic status, marital status, the respective PA score at baseline, smoking, body mass index, and morbidity.

Results
331 adults were evaluated. There was a decrease in the practice of PA in the occupational domain and an increase in the leisure/locomotion domain. Regarding HRQoL, there was an increase in the scores of body pain and mental health, and a decrease in scores of general health, vitality, social aspects and emotional aspects. The PA practice in the occupational domain was inversely related to functional capacity (β = − 7.2 [CI 95% − 13.0; − 1.4]). The practice of PA through sports in leisure time was positively associated with vitality (β = 5.5 [CI 95% 0.2; 10.7]) and mental health (β = 15.2 [CI 95% 6.8; 23.7]). PA practice during leisure and locomotion was inversely associated with functional capacity (β = − 5.68 [CI 95% − 10.7; − 0.6]) and positively associated with vitality β = 4.8 [CI 95% 0.8; 8.7]) and mental health (β = 8.4 [CI 95% 2.0; 14.9]). The total PA practice was inversely associated with functional capacity (β = − 3.8 [CI 95% − 6.5; − 1.2]) and positively associated with pain in the body (β = 4.9 [CI 95% 0.3; 9.4]), vitality (β = 2.9 [CI 95% 0.7; 5.0]) and mental health (β = 5.7 [CI 95% 2.2; 9.2]).

Conclusions
It is suggested that practicing PA continuously within a period of two years can positively affect some physical aspects and some mental aspects related to HRQoL, but not all of them. A strategy for public policy actions is to explore these variables by domain and thus detect the real needs and improvements that can be made for the population.
Macrophage-based delivery of interleukin-13 improves functional and histopathological outcomes following spinal cord injury
Authors: Van Broeckhoven Jana , Erens Céline , Sommer Daniela , Scheijen Elle , Sanchez Selien , Vidal Pia M. , Dooley Dearbhaile , Van Breedam Elise , Quarta Alessandra , Ponsaerts Peter , Hendrix Sven , Lemmens Stefanie 
Published: April 2022

Abstract
Background
Spinal cord injury (SCI) elicits a robust neuroinflammatory reaction which, in turn, exacerbates the initial mechanical damage. Pivotal players orchestrating this response are macrophages (Mφs) and microglia. After SCI, the inflammatory environment is dominated by pro-inflammatory Mφs/microglia, which contribute to secondary cell death and prevent regeneration. Therefore, reprogramming Mφ/microglia towards a more anti-inflammatory and potentially neuroprotective phenotype has gained substantial therapeutic interest in recent years. Interleukin-13 (IL-13) is a potent inducer of such an anti-inflammatory phenotype. In this study, we used genetically modified Mφs as carriers to continuously secrete IL-13 (IL-13 Mφs) at the lesion site.

Methods
Mφs were genetically modified to secrete IL-13 (IL-13 Mφs) and were phenotypically characterized using qPCR, western blot, and ELISA. To analyze the therapeutic potential, the IL-13 Mφs were intraspinally injected at the perilesional area after hemisection SCI in female mice. Functional recovery and histopathological improvements were evaluated using the Basso Mouse Scale score and immunohistochemistry. Neuroprotective effects of IL-13 were investigated using different cell viability assays in murine and human neuroblastoma cell lines, human neurospheroids, as well as murine organotypic brain slice cultures.

Results
In contrast to Mφs prestimulated with recombinant IL-13, perilesional transplantation of IL-13 Mφs promoted functional recovery following SCI in mice. This improvement was accompanied by reduced lesion size and demyelinated area. The local anti-inflammatory shift induced by IL-13 Mφs resulted in reduced neuronal death and fewer contacts between dystrophic axons and Mφs/microglia, suggesting suppression of axonal dieback. Using IL-4Rα-deficient mice, we show that IL-13 signaling is required for these beneficial effects. Whereas direct neuroprotective effects of IL-13 on murine and human neuroblastoma cell lines or human neurospheroid cultures were absent, IL-13 rescued murine organotypic brain slices from cell death, probably by indirectly modulating the Mφ/microglia responses.

Conclusions
Collectively, our data suggest that the IL-13-induced anti-inflammatory Mφ/microglia phenotype can preserve neuronal tissue and ameliorate axonal dieback, thereby promoting recovery after SCI.
Gut microbiota influence immunotherapy responses: mechanisms and therapeutic strategies
Authors: Lu Yuting , Yuan Xiangliang , Wang Miao , He Zhihao , Li Hongzhong , Wang Ji , Li Qin 
Published: April 2022

AbstractThe gut microbiota have long been recognized to play a key role in human health and disease. Currently, several lines of evidence from preclinical to clinical research have gradually established that the gut microbiota can modulate antitumor immunity and affect the efficacy of cancer immunotherapies, especially immune checkpoint inhibitors (ICIs). Deciphering the underlying mechanisms reveals that the gut microbiota reprogram the immunity of the tumor microenvironment (TME) by engaging innate and/or adaptive immune cells. Notably, one of the primary modes by which the gut microbiota modulate antitumor immunity is by means of metabolites, which are small molecules that could spread from their initial location of the gut and impact local and systemic antitumor immune response to promote ICI efficiency. Mechanistic exploration provides novel insights for developing rational microbiota-based therapeutic strategies by manipulating gut microbiota, such as fecal microbiota transplantation (FMT), probiotics, engineered microbiomes, and specific microbial metabolites, to augment the efficacy of ICI and advance the age utilization of microbiota precision medicine.
Autologous hematopoietic stem cell transplantation promotes connective tissue remodeling in systemic sclerosis patients
Authors: Zanin-Silva Djúlio C. , Santana-Gonçalves Maynara , Kawashima-Vasconcelos Marianna Y. , Lima-Júnior João R. , Dias Juliana B. E. , Moraes Daniela A. , Covas Dimas T. , Malmegrim Kelen C. R. , Ramalho Leandra , Oliveira Maria Carolina 
Published: April 2022

Abstract
Background
Autologous hematopoietic stem cell transplantation (AHSCT) treats patients with severe and progressive systemic sclerosis (SSc). However, basic mechanisms associated with the therapeutic efficacy of the procedure are not entirely understood. We aimed to evaluate how AHSCT affects skin fibrosis in SSc patients.

Methods
Clinical data, serum, and skin samples from 39 SSc patients who underwent AHSCT were retrospectively evaluated. Skin biopsies were analyzed by immunohistochemistry with anti-MMP-1, -MMP-2, -MMP-3, -MMP-9, -TIMP-1, -α-SMA, -TGF-β, and -NF-κB p65 antibodies, and stained with hematoxylin and eosin and picrosirius red to assess skin thickness and collagen density, respectively. Serum samples were evaluated by Multiplex Assay for COL1A1, COL4A1, FGF-1, MMP-1, MMP-3, MMP-12, MMP-13, PDGF-AA, PDGF-BB, S100A9, and TIMP-1 levels and compared to healthy controls.

Results
After AHSCT, SSc patients showed clinical improvement in skin involvement, assessed by modified Rodnan’s skin score (mRSS). Histologically, collagen density and skin thickness decreased after AHSCT. Immunohistochemical analyses showed increased expression of MMP-2, MMP-3, MMP-9, and TIMP-1 after AHSCT, whereas expression of NF-κB p65 decreased. At baseline, serum levels of COL4A1 and S100A9 were higher than in healthy controls. Serum levels of S100A9 normalized after AHCST in SSc patients compared to controls. Serum levels of PDGF-AA, PDGF-BB, TIMP-1, and MMP-1 decreased, while COL1A1 increased after AHSCT in SSc patients. No changes were detected in MMP-3, MMP-12, MMP-13, and FGF-1 serum levels after AHSCT.

Conclusions
Our results suggest that the therapeutic effects of AHSCT on skin fibrosis are related to changes in molecules associated with connective tissue maintenance and inflammation in SSc.
Hyperspectral time series datasets of maize during the grain filling period
Authors: Craig Valerie , Earl Hugh , Sulik John , Lee Elizabeth A. 
Published: April 2022

Abstract
Objectives
Remotely sensed hyperspectral data are increasingly being used to assess crop development and growth throughout the growing season. Large datasets capturing key growth stages can be useful to researchers studying many physiological plant responses. A time series analysis of hyperspectral reflectance measurements taken during the grain filling period and published within a publicly accessible database are described herein. These datasets document the spectral reflectance pattern of the canopy within the visible and near-infrared portion of the electromagnetic spectrum during the late stages of the grain filling period as plants approach and reach physiological maturity.

Data description
Included within the data repository are canopy-level hyperspectral datasets collected in 2017 and 2018. Data is included in its raw form, as well as with several manipulations to smooth and standardize the raw data. Data are released as comma separated value spreadsheets as well as Microsoft Excel open XLSX spreadsheets. These are accompanied by README text files which further describe the data and supplemental files that record hybrids used and plant phenology for each year of data collection.
Batch-effect detection, correction and characterisation in Illumina HumanMethylation450 and MethylationEPIC BeadChip array data
Authors: Ross Jason P. , van Dijk Susan , Phang Melinda , Skilton Michael R. , Molloy Peter L. , Oytam Yalchin 
Published: April 2022

AbstractBackgroundGenomic technologies can be subject to significant batch-effects which are known to reduce experimental power and to potentially create false positive results. The Illumina Infinium Methylation BeadChip is a popular technology choice for epigenome-wide association studies (EWAS), but presently, little is known about the nature of batch-effects on these designs. Given the subtlety of biological phenotypes in many EWAS, control for batch-effects should be a consideration.ResultsUsing the batch-effect removal approaches in the ComBat and Harman software, we examined two in-house datasets and compared results with three large publicly available datasets, (1214 HumanMethylation450 and 1094 MethylationEPIC BeadChips in total), and find that despite various forms of preprocessing, some batch-effects persist. This residual batch-effect is associated with the day of processing, the individual glass slide and the position of the array on the slide. Consistently across all datasets, 4649 probes required high amounts of correction. To understand the impact of this set to EWAS studies, we explored the literature and found three instances where persistently batch-effect prone probes have been reported in abstracts as key sites of differential methylation. As well as batch-effect susceptible probes, we also discover a set of probes which are erroneously corrected. We provide batch-effect workflows for Infinium Methylation data and provide reference matrices of batch-effect prone and erroneously corrected features across the five datasets spanning regionally diverse populations and three commonly collected biosamples (blood, buccal and saliva).ConclusionsBatch-effects are ever present, even in high-quality data, and a strategy to deal with them should be part of experimental design, particularly for EWAS. Batch-effect removal tools are useful to reduce technical variance in Infinium Methylation data, but they need to be applied with care and make use of post hoc diagnostic measures.
On the characterization of tidal ocean-dynamo signals in coastal magnetic observatories
Authors: Petereit Johannes , Saynisch-Wagner Jan , Morschhauser Achim , Pick Leonie , Thomas Maik 
Published: April 2022

AbstractPeriodic tidal ocean currents induce electric currents and, therefore, magnetic field signals that are observable using spaceborne and ground-based observation techniques. In theory, the signals can be used to monitor oceanic temperature and salinity variations. Tidal magnetic field amplitudes and phases have been extracted from magnetometer measurements in the past. However, due to uncertainties caused by a plentitude of influencing factors, the shape and temporal variation of these signals are only known to a limited extent. This study uses past extraction methods to characterize seasonal variations and long-term trends in the ten year magnetometer time series of three coastal island observatories. First, we assess data processing procedures used to prepare ground-based magnetometer observations for tidal ocean dynamo signal extraction to demonstrate that existing approaches, i.e., subtraction of core field models or first-order differencing, are unable to reliably remove low-frequency contributions. We hence propose low-frequency filtering using smoothing splines and demonstrate the advantages over the existing approaches. Second, we determine signal and side peak magnitudes of the M2 tide induced magnetic field signal by spectral analysis of the processed data. We find evidence for seasonal magnetic field signal variations of up to $$25\%$$

25
%

from the annual mean. Third, to characterize the long-term behavior of tidal ocean dynamo signal amplitudes and phases, we apply different signal extraction techniques to identify tidal ocean-dynamo signal amplitudes and phases in sub-series of the ten-year time series with incrementally increasing lengths. The analyses support three main findings: (1) trends cause signal amplitude changes of up to $$\approx 1~$$

≈
1

nT and phase changes are in the order of $${\mathcal {O}}(10^{\circ }$$

O
(

10
∘

) within the observation period; (2) at least four years of data are needed to obtain reliable amplitude and phase values with the extraction methods used and (3) signal phases are a less dependent on the chosen extraction method than signal amplitudes.
Graphical Abstract
nNav1.5 expression is associated with glutamate level in breast cancer cells
Authors: Azahar Irfan Irsyad , Sharudin Nur Aishah , Noor Din Ahmad Hafiz Murtadha , Che Has Ahmad Tarmizi , Mohd Nafi Siti Norasikin , Jaafar Hasnan , Mokhtar Noor Fatmawati 
Published: April 2022

Abstract
Background
Glutamate and voltage-gated sodium channels, both have been the target of intense investigation for its involvement in carcinogenesis and progression of malignant disease. Breast cancer with increased level of glutamate often metastasize to other organs (especially bone), whilst re-expression of ‘neonatal’ Nav1.5, nNav1.5 in breast cancer is known to promote cell invasion in vitro, metastasis in vivo and positive lymph node metastasis in patients.

Methods
In this study, the role of nNav1.5 in regulating glutamate level in human breast cancer cells was examined using pharmacological approach (VGSCs specific blocker, TTX, glutamate release inhibitor, riluzole and siRNA-nNav1.5). Effect of these agents were evaluated based on endogenous and exogenous glutamate concentration using glutamate fluorometric assay, mRNA expression of nNav1.5 using qPCR and finally, invasion using 3D culture assay.

Results
Endogenous and exogenous glutamate levels were significantly higher in aggressive human breast cancer cells, MDA-MB-231 cells compared to less aggressive human breast cancer cells, MCF-7 and non-cancerous human breast epithelial cells, MCF-10A. Treatment with TTX to MDA-MB-231 cells resulted in significant reduction of endogenous and exogenous glutamate levels corresponded with significant suppression of cell invasion. Subsequently, downregulation of nNav1.5 gene was observed in TTX-treated cells.

Conclusions
An interesting link between nNav1.5 expression and glutamate level in aggressive breast cancer cells was detected and requires further investigation.
Prematurity and low birth weight: geospatial analysis and recent trends
Authors: Peterman Nicholas , Kaptur Bradley , Lewis Morgan , Ades Lindsey , Carpenter Kristine 
Published: April 2022

AbstractPrematurity and low birth weight are of concern in neonatal health. In this work, geospatial analysis was performed to identify the existence of statistically significant clusters of prematurity and low birth weight using Moran’s I. Data was obtained from March of Dimes and the National Center for Health Statistics for the years 2015 to 2019. Analysis demonstrated the presence of hotspot (High-High) and coldspot (Low-Low) geographic clusters of these variables in regions across the United States. Additionally, factorial ANOVA was performed, and revealed the significance of demographic variables of interest. Given the strong relationship between these two variables, regions that are hotspots for one variable, but not the other, are of particular interest for further study.
Roadmap to Ventral Craniocervical Junction Through the Endonasal Corridor: Anatomic Evaluation of Inverted U-Shaped Nasopharyngeal Flap Exposure in a Cadaveric Study
Authors: Forbes Jonathan A. , Nebor Ivanna , Hussein Ahmad , Woodhouse Cody , Ceja David Gallardo , Avendano-Pradel Rafael , Andaluz Norberto , Sedaghat Ahmad R. , Phillips Katie , Prestigiacomo Charles , Virojanapa Justin , Cheng Joseph , Rosito Diego Mendez , Zuccarello Mario 
Published: April 2022

BACKGROUND:
There is a paucity of data in the literature describing quantitative exposure of the ventral craniocervical junction through the endonasal corridor in a safe manner mindful of locoregional anatomy.

OBJECTIVE:
To quantify ventromedial exposure of O-C1 and C1-2 articular structures after turning an inverted U-shaped nasopharyngeal flap (IUNF) and to obtain measurements assessing the distance of flap margins to adjacent neurovascular structures.

METHODS:
In 8 cadaveric specimens, an IUNF was fashioned using a superior incision below the level of the pharyngeal tubercule of the clivus and lateral incisions in the approximate region of Rosenmuller fossae bilaterally. Measurements with calipers and/or neuronavigation software included flap dimensions, exposure of O-C1 and C1-2 articular structures, inferior reach of IUNF, and proximity of the internal carotid artery (ICA) and hypoglossal nerve to IUNF margins.

RESULTS:
The IUNF facilitated exposure of an average of 9 mm of the medial surfaces of the right/left O-C1 joints without transgression of the carotid arteries or hypoglossal nerves. The C1-2 articulation could not be routinely accessed. The margins of the IUNF were not in close (<5 mm) proximity to the ICA in any of the 8 specimens. In 6 of 8 specimens, the dimensions of the IUNF were in close (<5 mm) horizontal or vertical proximity to the hypoglossal foramina.

CONCLUSION:
The IUNF provided safe and reliable access to the medial O-C1 articulation. Given the close proximity of the exocranial hypoglossal foramen, neuronavigation assistance and neuromonitoring with attention to the superolateral IUNF margin are recommended.
Laser-mediated osteoblast ablation triggers a pro-osteogenic inflammatory response regulated by reactive oxygen species and glucocorticoid signaling in zebrafish
Authors: Geurtzen Karina , López-Delgado Alejandra Cristina , Duseja Ankita , Kurzyukova Anastasia , Knopf Franziska 
Published: April 2022

ABSTRACTIn zebrafish, transgenic labeling approaches, robust regenerative responses and excellent in vivo imaging conditions enable precise characterization of immune cell behavior in response to injury. Here, we monitored osteoblast-immune cell interactions in bone, a tissue which is particularly difficult to in vivo image in tetrapod species. Ablation of individual osteoblasts leads to recruitment of neutrophils and macrophages in varying numbers, depending on the extent of the initial insult, and initiates generation of cathepsin K+ osteoclasts from macrophages. Osteoblast ablation triggers the production of pro-inflammatory cytokines and reactive oxygen species, which are needed for successful macrophage recruitment. Excess glucocorticoid signaling as it occurs during the stress response inhibits macrophage recruitment, maximum speed and changes the macrophage phenotype. Although osteoblast loss is compensated for within a day by contribution of committed osteoblasts, macrophages continue to populate the region. Their presence is required for osteoblasts to fill the lesion site. Our model enables visualization of bone repair after microlesions at single-cell resolution and demonstrates a pro-osteogenic function of tissue-resident macrophages in non-mammalian vertebrates.
Autophagy promotes organelle clearance and organized cell separation of living root cap cells in Arabidopsis thaliana
Authors: Goh Tatsuaki , Sakamoto Kaoru , Wang Pengfei , Kozono Saki , Ueno Koki , Miyashima Shunsuke , Toyokura Koichi , Fukaki Hidehiro , Kang Byung-Ho , Nakajima Keiji 
Published: April 2022

ABSTRACT
The root cap is a multilayered tissue covering the tip of a plant root that directs root growth through its unique functions, such as gravity sensing and rhizosphere interaction. To maintain the structure and function of the root cap, its constituent cells are constantly turned over through balanced cell division and cell detachment in the inner and outer cell layers, respectively. Upon displacement toward the outermost layer, columella cells at the central root cap domain functionally transition from gravity-sensing cells to secretory cells, but the mechanisms underlying this drastic cell fate transition are largely unknown. Here, using live-cell tracking microscopy, we show that organelles in the outermost cell layer undergo dramatic rearrangements. This rearrangement depends, at least partially, on spatiotemporally regulated activation of autophagy. Notably, this root cap autophagy does not lead to immediate cell death, but is instead necessary for organized separation of living root cap cells, highlighting a previously undescribed role of developmentally regulated autophagy in plants.
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