bluuebunny/crossref_metadata_2025_split huggingface dataset:
Features and Outcomes of Rectal Cancer Patients Treated in a Hospital in Bogotá, Colombia: a Retrospective Cohort Study
Authors: Romo Julián A. , Figueroa Carlos , López Laura A. , Mesa Natalia , González-Muñoz Alejandro , Baquero David , Recamán Andrea , Rabeya Fernando , Villabon Alejandro , Sánchez Isabella Velandia , Flechas Álvaro 
Published: September 2022

Abstract
Rectal cancer is an increasing disease worldwide. The outcomes of its treatment are related to the preoperative characteristics of the patient. The objective of this study was to describe sociodemographic, clinical and surgical characteristics and outcomes of patients operated on for rectal cancer at Hospital Universitario Mayor Méderi (HUM) during the period within 2013-2017.A retrospective descriptive cohort-type study was carried out by consulting the clinical records of patients above the age of 18 years with a clinical / histopathological diagnosis of rectal cancer and an institutional follow-up in those who underwent surgery with laparoscopic anterior resection of the rectum carried out by the coloproctology service of the HUM between 2013 and 2017. For statistical analysis, the SPSS V22 program was used.Data from 133 patients were collected during the study period, most of them male, with more frequent involvement of the lower rectum. Complications occurred in 25% of the patients. Conversion rate to open surgery was 8.6%, in-hospital death was associated with cardiovascular comorbidity, corticosteroid uses and with the presence of complications. Sociodemographic characteristics of the patients were similar to the world population. The institution has a low prevalence of anastomotic dehiscence, global complications are comparable with international statistics.
Phytochemical screening and in-vitro biological properties of unprocessed and household processed fenugreek (Trigonella foenum- graecum Linn.) seeds and leaves
Authors: El-Salam Shaimaa G.S . Abd , Rashed Mohamed M. , Ibrahim Nabih A. , Rahim Emam Abdel , Aly Tahany A. A. , AL-Farga Ammar 
Published: September 2022

Abstract
The impact of household processes on fenugreek leaves and seeds was analyzed for total phenolic (TP) and total flavonoid content (TF), and in-vitro biological activities such as antioxidant, antimicrobial, and anti-inflammatory properties. Processes included air-drying of leaves and germinating, soaking, and boiling of seeds. Air-dried fenugreek leaves (ADFL) had high TP (15.27 mg GAE/g D.W.) and TF (7.71 mg QE/g D.W.). The TF of unprocessed, germinated, soaked, and boiled seeds had 6.54, 5.60, 4.59, and 3.84 mg GAE/g D.W., respectively. The TF in UFS, GFS, SFS, and BFS were 4.23, 2.11, 2.10, and 2.33 mg QE/g D.W., respectively. Sixteen phenolic and nineteen flavonoid compounds were identified using the HPLC. Antioxidant activity using DPPH•, ABTS+•, and FRAP• assays indicated that ADFL had high activity. Antimicrobial activity was evaluated against each eight pathogenic bacterial and fungal strains. ADFL showed a strong activity with MIC values ranging from 0.03 to 1.06 and 0.04 to 1.18 mg ml− 1 against bacterial and fungal strains, respectively. Anti-inflammatory activity was evaluated in-vitro against RAW 264.7 macrophage cells using of NO assay. Results revealed that ADFL had the highest cytotoxicity and anti-inflammatory activity according to NO assay. Household processes significantly declined the in-vitro biological properties of processed seeds.
Techno-economic evaluation of electrocoagulation for Cattell slaughterhouse wastewater treatment using aluminum electrodes in batch and continuous experiment
Authors: Hellal Mohamed Saad , Doma Hala Salah , Abou-Taleb Enas Mohamed 
Published: September 2022

Abstract
This study aimed to investigate, from technoeconomic point of view, cattle slaughterhouse wastewater (CSWW) treatment via electrocoagulation (EC) technique. A novel lab scale electrocoagulation unit with 3L volume was manufactured and tested. The EC unite contains nine identical cylindrical shape electrodes from aluminum material in connection with controllable DC power supply. Investigation of optimum operating parameters in terms of pH, current density (CD), contact time and electrolyte concentration (TDS) was carried out in batch mode then applied for continuous mode. At each batch, cost analysis was calculated in terms of consumption electrode material and electrical power. The optimum operating conditions at which best removal rate achieved was pH 7, contact time 75 min, TDS = 3000 mg L− 1 and CD of 4 mA.cm− 2. After application of these conditions on continuous flow mode, the removal rate of Chemical oxygen demand (COD), color, turbidity, biological oxygen demand (BOD), and oil, grease were 95.3%, 99.8%, 98.7%, 96.5% and 94.5% respectively. The total electrode consumption and electrical consumption were 0.6 kg m− 3 and 0.87kWh m− 3 with operational cost about 1.5$ m− 3. This proved that EC is techno-economically effective treatment method than other conventional treatment methods for high-rate removal of pollutants of from CSWW.
No evidence for a common blood microbiome based on a population study of 9,770 healthy humans
Authors: Tan Cedric , Chia Minghao , Ko Karrie , Chen Hui , Liu Jianjun , Loh Marie , Nagarajan Niranjan 
Published: September 2022

Abstract
Human blood is conventionally considered sterile. Recent studies have challenged this, suggesting the presence of a blood microbiome in healthy humans. We present the largest investigation to date of microbes in blood, based on shotgun sequencing libraries from 9,770 healthy subjects. Leveraging the availability of data from multiple cohorts, we stringently filtered for laboratory contaminants to identify 117 microbial species detected in the blood of sampled individuals, some of which had signatures of DNA replication. These primarily comprise of commensals associated with human body sites such as the gut (n=40), mouth (n=32), and genitourinary tract (n=18), which are species that are distinct from common pathogens detected in clinical blood cultures based on more than a decade of records from a tertiary hospital. Contrary to the expectations of a shared blood microbiome, no species were detected in 84% of individuals, while only a median of one microbial species per individual was detected in the remaining 16%. Futhermore, microbes of the same species were detected in <5% of individuals, no co-occurrence patterns similar to microbiomes in other body sites was observed, and no associations between host phenotypes (e.g. demographics and blood parameters) and microbial species could be established. Overall, these results do not support the hypothesis of a consistent core microbiome endogenous to human blood. Rather, our findings support the transient and sporadic translocation of commensal microbes from other body sites into the bloodstream.
Outcomes of metaplastic breast cancer versus triple negative breast cancer: a propensity score matching analysis
Authors: Tan Yuping , Yang Biao , Chen YuHong , Yan Xi 
Published: September 2022

AbstractPurpose This study aims to compare the survival outcomes of MBC with triple-negative breast cancer (TNBC) and identify prognostic factors that influence MBC survival. Methods Patients with non-metastatic MBC or TNBC were reviewed from our database from 2002 to 2021. Patient clinicopathologic features and treatment were analyzed with respect to outcomes including disease progression-free survival (DFS), and overall survival (OS). Propensity score matching (PSM) with a one-to-three matching between MBC and TNBC was performed. Results A total number of 857 female patients (76 MBC and 781 TNBC) were included in this study, with median age in 49 years (28–77 years). A subgroup of triple negative MBC (n = 60) was matched with TNBC (n = 180) cases based on patient characteristics and treatment. OS and DFS were significantly worse in the MBC group before (p = 0.0046 both) and after (p = 0.011 and p = 0.0046, respectively) PSM. Multivariable analysis revealed larger tumor size (T > 5cm) (HR = 3.797, 95%CI 1.118–12.902, p = 0.032) and lymph nodal status (N3 vs N0-2, HR = 6.149 95%CI 1.499–25.229, p = 0.012) were associated with worse OS after PSM. Among the 76 MBC patients, higher T stage and mesenchymal differentiation were associated with worse overall survival (pT1/2 vs pT3/4 and mesenchymal differentiation vs other type, p = 0.007 and p = 0.011, respectively). Lymph node positive and mesenchymal differentiation were associated with worse disease-free survival (Figs. 5 and 6, p = 0.005 and p < 0.001, respectively). Conclusions Compared with TNBC, MBC tends to have a worse OS. Mesenchymal differentiation has a worse DFS than other subtypes of MBC.
Comparison of Malaria diagnostic methods for detection of asymptomatic Plasmodium infections among pregnant women attending antenatal care at Fendeka town health facilities, Jawi district, Northwest Ethiopia
Authors: Yohannis Adane Tilahun , Yimer Mulat , Gelaye Woynshet , Tegegne Banchamlak , Abebaw Abitie , Ayalew Demssie , Alamneh Demeke Endalamaw 
Published: September 2022

Abstract
Background
Malaria in pregnancy remains a major public health problem in the globe especially in sub-Saharan Africa. In malaria endemic areas, majority of pregnant women remain asymptomatic but could still associate with complications on the mother and her offspring. They also serve as reservoirs to transmit infection. Despite these effects, no attention is given for the diagnosis of asymptomatic Plasmodium infections (APIs) using highly sensitive and specific laboratory diagnostic tools among pregnant women in Ethiopia. Therefore, the aim of this study was to compare the performance of RDTs with microscopy and performance of RDTs and microscopy with PCR for detecting APIs among pregnant women attending antenatal care at Fendeka town health facilities, Jawi district, Northwest Ethiopia.
Methods
Health facility based cross -sectional study was conducted from February-March, 2019. A total of 166 participants were enrolled by using convenient sampling technique. Socio-demographic and other characteristics were collected using semi structured questionnaire. Dried blood spot (DBS) samples were collected for molecular analysis. APIs on pregnant women was diagnosed using rapid diagnostic tests (RDTs), smear microscopy and real-time polymerase chain reaction (RT-PCR). Data were analyzed using SPSS version 20. Descriptive statistics were used to determine the prevalence of APIs. Method comparison was done and Cohen’s kappa coefficient (k) was used to determine the degree of agreement among the diagnostic methods. Parasite densities were also calculated
Results
Prevalence of API was 17 (10.2%), 18 (10.8%) and 30 (18.1%) using RDTs, microscopy and real-time PCR, respectively. The overall proportion of API was 18.7%. Sensitivity of the RDT was 83.3% as compared with microscopy. RDTs and microscopy also showed sensitivity of 50% and 60%, respectively as compared with PCR. Majority of API identified by microscopy had moderate parasite densities.
Conclusions
Prevalence of API in the study area was significant. Both RDTs and microscopy had lower sensitivity when compared with real-time PCR. Therefore, routine laboratory diagnosis of API among pregnant women with better laboratory diagnostic tool would be adopted as a part of the antenatal care.
On periodic growth and shrinkage of glaciers in the Warwan sub-basin, western Himalaya between 1990 and 2020
Authors: Garg Purushottam Kumar , Malviya Apoorva , Shukla Aparna , Garg Siddhi , Singh Nilendu 
Published: September 2022

Abstract
Knowledge about glacier extent, dynamics and characteristics are important for climate change attribution and prediction. Understanding on long-term dynamics and glacier inventory is crucial, particularly for the melt-dominated and latitudinally-diverse western Himalayan glacier basins. In this study, a temporal inventory is prepared for Warwan-sub basin (WSB), utilizing satellite imageries since the 1993 (Landsat TM: 1993; ETM+: 2001, 2008; OLI: 2020) and elevation model (SRTM DEM: 2000). The base inventory was generated for the year 2001 and systematically adjusted to the glacier situations in 1993, 2008 and 2020. Results indicate that in the year 2001, WSB in the western Himalaya included 84 glaciers (> 0.02 km2) covering an area of 187.9 ± 5.8 km2. The mapping (2001) further revealed a supraglacial debris cover of 15% of the glacierized area (28.2 ± 0.9 km2). Overall, the debris cover increased by 6% between 1993 and 2020. Temporal analyses clearly suggest a period of gain in the glacierized area (2001–2008) interspersed by the two phases of decline (1993–2001 and 2008–2020). Results specify a stronger decline in the glacierized area during 1993 to 2001 (197.03 ± 6.1 to 187.9 ± 5.8 km2) than between 2008 and 2020 (188.4 ± 5.9 to 182.8 ± 5.66 km2). Remarkably, the glacierized area increased from 187.9 ± 5.8 to 188.4 ± 5.8 km2 during 2001 to 2008. In view of widespread recession of regional glaciers, the gain in the area between 2001 and 2008 represents a peculiar characteristic of WSB that needs further detailed investigation. Further analyses suggest that low-altitude, east-facing, debris-free, steep-sloped and small glaciers experienced greater loss in the area than large, debris-covered, north-facing, gently-sloped and high-altitude glaciers. Overall, the study at the sub-basin scale reveals inherent glacier dynamics with periodic increase and decrease in the glacierized area and a notable influence of non-climatic factors in regulating spatial heterogeneity and the rate of glacier changes.
ZIF-8 induced hydroxyapatite-like crystals enabled superior osteogenic ability of MEW printing PCL scaffolds
Authors: Wang Bingqian , Zeng Yuyang , Liu Shaokai , Zhou Muran , Fang Huimin , Wang Zhenxing , Sun Jiaming 
Published: September 2022

Abstract
Background

In general, ZIF-8 tends to undergo ion responsive degradation in ionic solutions, which makes it difficult to maintain its original structure, thus restricting its direct application in biological settings.

Methods

ZIF-8 was synthesized using one-pot method and the ZIF-8/PCL scaffolds were built by a round mould or printed by melt electrowrittten (MEW) technology. Mineralization performance was evaluated by SEM with EDS-mapping and micro-CT scanning after soaking in simulated body fluid (SBF). The osteogenic activity in vivo or in vitro was assessed using different methods including micro-CT scanning, Alizarin red staining, and immunohistochemical staining.

Results

Herein, an abnormal phenomenon is reported that ZIF-8 can form large hydroxyapatite-like crystals when getting immersed directly in multi-ion simulated body fluid. The abnormal crystals showed the continuation of rapid growth in 14 days, with its volume increasing by more than ten times. As suggested by the release of Zn2+ and the emergence of new XRD diffraction peaks, ZIF-8 particles might gradually collapse and congregate through competitive coordination and re-nucleation. The above phenomenon is also observable on the surface of ZIF-8/PCL composite materials and MEW printing ZIF-8/PCL scaffolds. ZIF-8 increased the roughness of PCL by altering its surface topography, which significantly improved its biocompatibility and osteoinductivity both in vitro and in vivo. Notably, the capability of pro-biomineralization makes ZIF-8 also applicable in polylactic acid.

Conclusions

To sum up, the results shown in this study demonstrate that ZIF-8 can serve as a bioactive additive that enables the surface modification to synthetic polymers, which suggests its potential of application for in-situ bone regeneration.
NPM1 mutation reprograms leukemic transcription network via reshaping TAD topology
Authors: Lai Qian , Hamamoto Karina , Luo Huacheng , Zaroogian Zachary , Lesperance Julia , Zha jie , Qiu Yi , Guryanova Olga , Huang Suming , Xu Bing 
Published: September 2022

Abstract
C-terminal mutation of Nucleophosmin 1 (NPM1C+) was thought to be a primary driving event in acute myeloid leukemia (AML) that reprograms leukemic-associated transcription programs to transform hematopoietic stem and progenitor cells (HSPCs). However, molecular mechanisms underlying NPM1C+-driven leukemogenesis remain elusive. Here, we report that NPM1C+ reprograms MIZ-1/MYC regulatory axis by altering NPM1-associated CTCF-driven topologically associated domains (TADs) that switches the balance of MIZ1 interaction with coactivator NPM1/p300 and corepressors MYC/G9A complexes to control cell cycle progression and myeloid lineage-specific PU.1/CEBPα transcription networks leading to impairment of myeloid differentiation. Hematopoietic-specific NPM1C+ knock-in alters TAD topology leading to disrupted regulation of the cell cycle and myeloid master transcription factors, chromatin accessibility, and gene expression, which results in myeloid differentiation block. Retention of NPM1 or re-expression of PU.1 or CEBPα within the nucleus restores differentiation programs by reorganizing TADs critical for myeloid TFs and cell cycle regulators, and prevents NPM1C+-driven leukemogenesis. In sum, our data reveal that NPM1C+ reshapes CTCF-defined TAD topology to reprogram signature leukemic transcription programs required for cell cycle progression and leukemic transformation. Restoration of myeloid transcription program in nucleus reversed NPM1C+-driven transcription signature and promotes myeloid differentiation leading to mitigation of AML.
Effects of age, unilateral renal ligation, and acute blood loss on a murine model of adenine-induced renal anemia
Authors: Han Dong , Huo Mengqi , Yu Jiawei , Li Dongxin , Ma Lin , Xu Huiying , Guo Min , Liu Xiaoxia , Liu Hongfang , Wang Xiangming 
Published: September 2022

Abstract
To explore the effects of age and unilateral renal ligation on hemoglobin levels, and the effects of acute bleeding on endogenous erythropoietin (EPO) in renal anemia. Adenine-induced mice were established to analyze the effects of age, acute blood loss (12-week-old and 12-month-old) and unilateral renal ligation (8-month-old) on renal anemia. The reasons changing serum EPO concentration were investigated by liver, kidney tests etc. It was found that the hemoglobin level of 12-month-old mice was lower than that of 12-week-old mice, and the hemoglobin level of unilateral renal ligation plus adenine was lower than that of adenine alone (p < 0.01). After bleeding, the levels of serum EPO in two model group were significantly higher than their corresponding normal groups (p < 0.01), although there was no statistical difference between any two groups in non-bleeding mice (p > 0.05). Renal EPO expression in model group decreased significantly compared with the normal group (p < 0.01), but the hepatic EPO expression increased significantly (p < 0.01), which were not only related with hemorrhage, but consistent with the expression of hypoxia-inducing factor-2α (HIF-2α). In summary, age and unilateral renal ligation aggravate renal anemia. They, together with hemorrhage, activate endogenous EPO secretion in liver.
Position and stiffness control of an antagonistic variable stiffness actuator with input delay using super-twisting sliding mode control
Authors: Javadi Ali , Chaichaowarat Ronnapee 
Published: September 2022

Abstract
Motor dynamics in Antagonistic Variable Stiffness Actuator (AVSA) is generally not considered in control system design. This ignorance can lead to an inaccurate system model and hence the performance of closed-loop system can be affected. The motor dynamics can be modeled as an input-delay in actuator model. For the first time, motor dynamics is modeled as input time-delay for an antagonistic variable stiffness actuator in this paper. Since the stiffness of AVSA is a nonlinear function of system states, stiffness tracking for AVSA is a challenging task. Especially when the model contains input delay, many of the existing delay compensation controllers cannot be used for stiffness tracking purpose. To handle this issue, a nonlinear transformation is introduced and then a super-twisting sliding mode control is utilized to reach position and stiffness tracking simultaneously. To compensate input time-delay, prediction-based feedback is involved together with disturbance observers for estimating external disturbance. Simulation results show that the proposed design approach is successful in position and stiffness tracking and at the same time in attenuating external disturbance effect.
Cancer survivors present physical and psychological sequelae after COVID-19 hospitalization: a prospective and longitudinal cross-sectional study assessing post-acute sequelae of SARS-CoV-2 infection (PASC) in cancer patients
Authors: Leite Victor Figueiredo , Lourenço Maria Teresa Duarte Pereira da Cruz , Tarabay Christina Haas , Mori Agnes Ayumi Sewo , Neto Giseli Maria , Miola Thais Manfrinato , Costa Luciana da , Friedrich Celena Freire , Rodrigues Telma Ribeiro , Balbinot Jordana , Angelis Elisabete Carrara 
Published: September 2022

Abstract
Purpose: to prospectively assess symptoms and impairments following COVID-19 hospitalization in a cancer population, particularly regarding fatigue, nutritional status, speech and swallowing, anxiety, depression, and post-traumatic stress disorder (PTSD).Methods: Prospective and longitudinal cross-sectional study with cancer survivors hospitalized due to COVID-19 from June to December 2020. Participants were assessed at discharge, and after one, three, six and twelve months. Results: We included 51 participants. At the time of discharge, 84.3% participants presented at least one impairment: fatigue (68.6%), anxious/depressive symptoms (23.5%), voice and speech impairments (19.6%), dysphagia (21.6%), and were at nutritional risk (54.9%). After one year, impairments were present in 53.7% of the sample: fatigue (34.2%), anxious/depressive symptoms (39.2%), voice and speech impairments (29.4%), dysphagia (33.3%). No participants presented nutritional risk after one year. Participants had no significant impact on their activities of daily living (ADL).Conclusion: Cancer survivors hospitalized due to COVID-19 in our institution presented high levels of fatigue, nutritional risk, anxious/depressive and PTSD symptoms at the time of discharge, with improvement of fatigue, and some aggravation of speech, swallowing and psychological symptoms in the following year.
Pandemic-related changes in social interaction predict automatic approach-avoidance behaviour
Authors: Henwood Amanda , Rinck Mike , Krpan Dario 
Published: September 2022

Abstract
People’s natural tendencies to either approach or avoid different stimuli in their environment are considered fundamental motivators of human behaviour. There is a wealth of research exploring how changes in approach and avoidance motivational orientations impact behaviour with consequences for wellbeing. However, research has seldom explored this relationship in reverse. The COVID-19 pandemic offered a unique opportunity to explore whether widespread changes in social behaviour predict changes in automatic approach-avoidance tendencies over time. We gathered data on people’s adherence to 7 of the prescribed social behaviours set out by the UK government and people’s automatic approach-avoidance tendencies in response to different stimuli (sad/happy/neutral faces and social scenes) at three time points during the COVID-19 pandemic. Reduced-overall-interaction (digital and in person) was found to predict faster avoidance relative to approach of sad faces. The results suggest that automatic approach-avoidance tendencies may function to counterbalance the negative impacts of reduced social interaction, with important implications for understanding public resilience during time of crisis, and beyond.