bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hydroxycitric acid inhibits oxalate nephropathies formation through crystallization regulation and activation of the PPARα pathway
Authors: Zhang Yi-Han , Li Shu-Jue , Yu Bang-Xian , Liang Qing , Sun Xin-Yuan 
Published: December 2022

AbstractOxalate-induced nephropathies comprise a range of kidney disorders, for which there are no efficient pharmacological treatments. Hydroxycitric acid (HCA) is a derivative of citric acid with a variety of pharmacological activities including reducing body weight and calcium salt deposition. However, the specific mechanism of inhibition of oxalate nephropathies by this compound is not well understood. In this study, we successfully applied bioinformatics-based and simulated drug molecular docking approaches to predict potential targets of HCA. Subsequently, we explored the molecular mechanisms of HCA inhibition of renal calcium oxalate (CaOx) deposition and nephrotoxicity in an oxalate-induced NRK-52E cell model and an oxalate nephropathy rat model. HCA could effectively inhibit CaOx crystal deposition and reduce crystal adhesion and oxidative damage, effectively inhibit lipid deposition caused by high oxalate, and reduce lipid nephrotoxicity. HCA is more effective than traditional stone medications in inhibiting CaOx deposition and kidney damage. Further cellular transcriptomic analysis and in vitro results showed that HCA could stably bind peroxisome proliferator-activated receptor α (PPARα) and promote PPARα-RXR heterodimer formation, thus promoting the expression of downstream oxidative stress molecules (Nrf2, HO-1, SOD) and inhibiting calcium ion release and mitochondrial dysfunction, thus reducing oxalate-induced renal lipid peroxidation damage. Therefore, HCA, a novel drug with the ability to modulate lipid metabolism and inhibit CaOx formation, may be a therapeutic option for the treatment of oxalate nephropathies.
Sniper2L, a high-fidelity Cas9 variant with high activity
Authors: Kim Young-hoon , Kim Nahye , Okafor Ikenna , Choi Sungchul , Min Seonwoo , Lee Joonsun , Choi Keunwoo , Choi Janice , Harihar Vinayak , Kim Youngho , Kim Jin-Soo , Lee Jungjoon K. , Ha Taekjip , Kim Hyongbum Henry 
Published: December 2022

AbstractAlthough several high-fidelity SpCas9 variants that have reduced activities at mismatched target sequences have been reported, it has been observed that this increased specificity is associated with reduced on-target activity, limiting the applications of the high-fidelity variants when efficient genome editing is required. Here, we developed an improved version of Sniper-Cas9, Sniper2L, which represents an exception to this trade-off trend as it showed higher specificity with retained high activity. We evaluated Sniper2L activities at a large number of target sequences, and developed DeepSniper, a deep-learning model that can predict the activity of Sniper2L. We also confirmed that Sniper2L can induce highly efficient and specific editing at a large number of target sequences when it is delivered as a ribonucleoprotein complex. Mechanically, the high specificity of Sniper2L originates from its superior ability to avoid unwinding a target DNA containing even a single mismatch. We envision that Sniper2L will be useful when efficient and specific genome editing is required.
Aβ-CT affective touch: Touch pleasantness ratings for gentle stroking and deep pressure exhibit dependence on A-fibers
Authors: Case Laura K. , Madian Nicholas , McCall Micaela V , Bradson Megan L , Liljencrantz Jaquette , Goldstein Benjamin , Alasha Vince J , Zimmerman Marisa S 
Published: December 2022

AbstractGentle stroking of the skin is a common social touch behavior with positive affective consequences. A preference for slow versus fast stroking of hairy skin has been closely linked to the firing of unmyelinated C-tactile (CT) somatosensory afferents. Because the firing of CT afferents strongly correlates with touch pleasantness, the CT pathway has been considered a social-affective sensory pathway. Recently, ablation of the spinothalamic pathway-thought to convey all C-fiber sensations-in patients with cancer pain impaired pain, temperature, and itch, butnotratings of pleasant touch. This suggested integration of A and CT fiber input in the spinal cord, or A-fiber contributions to computations of touch pleasantness in the brain. However, the causal contribution of A-fibers to touch pleasantness- in humanswithoutpain-remains unknown. In the current, single-blinded study we performed two types of peripheral nerve blocks in healthy adults to temporarily eliminate the contribution of A-fibers to touch perception. Our findings show that when A-fiber function is greatly diminished, the perceived intensityandpleasantness of both gentle stroking and deep pressure are nearly abolished. These findings demonstrate that explicit perception of the pleasantness of CT-targeted brushing and pressure both critically depend on A-fibers.
Layer-dependent activity in the human superior colliculus during working memory
Authors: Chen Danlei , Kragel Philip A. , Wager Tor D. , Wald Lawrence L. , Bianciardi Marta , Satpute Ajay B. , Quigley Karen S. , Barrett Lisa Feldman , Katsumi Yuta , Theriault Jordan E. 
Published: December 2022

AbstractWe examined the superior colliculus (SC) with ultra-high resolution 7-Tesla fMRI during an N-back working memory task. We observed both increased BOLD signal intensity and functional connectivity that followed a layer-dependent pattern predicted from anatomical connections between SC and other brain structures important for visual processing, motor control, and executive function. Our results highlight a role for the human SC in cognitive functions that usually associated with the cerebral cortex.
Cellular production of ade novomembrane cytochrome
Authors: Hardy Benjamin J , Martin Hermosilla Alvaro , Chinthapalli Dinesh K , Robinson Carol V , Anderson JL Ross , Curnow Paul 
Published: December 2022

ABSTRACTHeme-containing integral membrane proteins are at the heart of many bioenergetic complexes and electron transport chains. The importance of these electron relay hubs across biology has inspired the design ofde novoproteins that recreate their core features within robust, versatile and tractable protein folds. To this end, we report here the computational design and in-cell production of a minimal diheme membrane cytochrome which successfully integrates into the cellular membrane of live bacteria. This synthetic construct emulates a four-helix bundle found in modern respiratory complexes but has no sequence homology to any polypeptide sequence found in nature. The twob-type hemes, which appear to be recruited from the endogenous heme pool, have distinct split redox potentials with values close to those of natural membrane-spanning cytochromes. The purified protein can engage in rapid biomimetic electron transport with small molecules, with other redox proteins, and with biologically-relevant diffusive electron carriers. We thus report an artificial membrane metalloprotein with the potential to serve as a functional module in electron transfer pathways in both synthetic protocells and living systems.
Syngap1 Regulates Cortical Circuit Assembly by Controlling Membrane Excitability
Authors: Arora Vineet , Michaelson Sheldon , Aceti Massimiliano , Kilinic Murat , Miller Courtney , Rumbaugh Gavin 
Published: December 2022

SummaryGene expression intersects with neural activity to produce cortical circuits during brain development. However, the cell biological mechanisms linking gene expression to activity-dependent cortical circuit assembly remain unclear. Here, we demonstrate in mice that a newly discovered function of the neurodevelopmental disorder gene,Syngap1, is to cell-autonomously control intrinsic membrane excitability (IME) in developing cortical glutamatergic neurons.Syngap1regulation of IME was mechanistically linked to wiring of a cortical circuit motif required for sensory processing and behavioral action. Restoring depressed IME inSyngap1deficient neurons through genetic targeting of hyper-functional potassium currents unleashed deficient dendritic morphogenesis in upper lamina sensory cortex pyramidal neurons. Furthermore, enhancing dendritic morphogenesis was sufficient to stimulate assembly of translaminar feed-forward excitatory circuit motifs. Thus,Syngap1promotes excitatory circuit assembly during cortical development by maintaining IME in a range that enables trophic neuronal activity to maximize pyramidal cell somatodendritic maturation and subsequent synapse formation.HighlightsSyngap1cell-autonomously tunes cortical pyramidal neuron IMEin vivoSyngap1-IME is regulated in part by control of neuronal potassium currentsSyngap1enhancement of IME drives dendritic maturation in pyramidal cellsSyngap1tuning of IME-regulated dendritic maturation promotes circuit assembly
The utility and caveat of split-GAL4s in the study of neurodegeneration
Authors: Stickley Luca , Nagoshi Emi 
Published: December 2022

AbstractParkinson’s disease (PD) is the second most common neurodegenerative disorder, afflicting over 1% of the population of age 60 years and above. The loss of dopaminergic (DA) neurons is the substantia nigra pars compacta (SNpc) is the primary cause of its characteristic motor symptoms. Studies usingDrosophila melanogasterand other model systems have provided much insight into the pathogenesis of PD. However, little is known why certain cell types are selectively susceptible to degeneration in PD. Here we describe an approach to identify vulnerable subpopulations of neurons in the genetic background linked to PD inDrosophila, using the split-GAL4 divers that enable genetic manipulation of a small number of defined cell population. We identify a subtype of DA neurons selectively vulnerable in the model of theleucine-rich repeat kinase 2(LRRK2)-linked familial PD, demonstrating the utility of this approach. We also show an unexpected caveat of the split-GAL4 system in aging-related research: the age-dependent increase in the number of GAL4-labeled cells.
Novel populations of Tr1 cells contribute to the resolution of acute influenza A virus infection
Authors: Abbott Caitlin A , Freimayer Emily L , Tyllis Timona S , Norton Todd S , Alsharifi Mohammed , Heng Aaron H S , Pederson Stephen M , Qu Zhipeng , Armstrong Mark , Hill Geoffrey R , McColl Shaun R , Comerford Iain 
Published: December 2022

AbstractType I regulatory (Tr1) cells contribute to immune suppression in the context of chronic infection, autoimmunity, and transplant tolerance. However, their physiological relevance in the resolution of acute respiratory infection is not understood. Here, we identify Tr1 cells accumulating in the lung parenchyma during resolution of the response to sublethal influenza A virus infection in mice. Tr1 cells were dependent on IL-27Rα and in their absence recovery from IAV-induced weight loss is impaired. Notably, these Tr1 cells did not necessarily co-express the typical Tr1 markers LAG-3 and CD49b, with four distinct populations of Tr1 cells apparent in the lungs. Each population was suppressive and were differentially dependent on IL-10 to mediate suppression. Transcriptional analysis revealed a core Tr1 gene signature in each population and distinct expression profiles indicative of different states of activation and differentiation. Finally, sort-transfer experiments indicated non-linear plasticity between these subsets of Tr1 cells. Together, these data support Tr1 cells contributing to the resolution of acute inflammation and define novel Tr1 cell phenotypes in acute infection.
The role of local news in constructing media legitimacy: how news media frames the sociopolitical efforts of multinational corporations in host countries
Authors: Marschlich Sarah , Ingenhoff Diana 
Published: December 2022

PurposeFor corporate communications, it is crucial to know how news media outlets report and frame the sociopolitical activities of multinational corporations (MNCs), including their corporate diplomacy, that affect perceptions of their legitimacy. Therefore, this study aims to identify how local news media frame corporate diplomacy in a host country and, in turn, benefit the media legitimacy of MNCs.Design/methodology/approachTo identify media frames in the host country, a quantitative content analysis involving factor and cluster analyses of 385 articles published in newspapers in the United Arab Emirates from 2014 to 2019 addressing the corporate diplomacy of large European MNCs operating in the country was conducted.FindingsThis study identified three media frames, two of which establish moral and pragmatic media legitimacy. Results suggest that media legitimacy grows when news media emphasise institutional relationships between MNCs and local, established organisations and corporate diplomacy's benefits for society.Practical implicationsFindings provide insights into how corporate communications can contribute to legitimacy building by emphasising corporations' relationships with institutional actors in host countries and the benefits of corporate activities for local communities.Originality/valueTo the best of the authors’ knowledge, this study was the first in corporate communications to empirically investigate news media's role in corporate diplomacy and how media frames contribute to the media legitimacy of MNCs at the moral, pragmatic, regulative and cognitive levels.
What is the impact of social well-being factors on happiness?
Authors: Trabelsi Mohamed Ali 
Published: December 2022

PurposeThe purpose of this study is to examine the effect of social support, healthy life expectancy, freedom to make life choices, generosity, corruption perception, real gross domestic product per capita and the Gini index on happiness.Design/methodology/approachIn this study, the sample consists of 137 countries observed over the period 2017–2019. A multidimensional approach is used consisting of a principal component analysis and an econometric linear regression model.FindingsThe findings indicate that perception, taking care of other people, corruption perception, freedom to make life choices and healthy life expectancy are the most determining factors of social well-being.Practical implicationsWell-being benefits countries by improving living standards. Indeed, taking care of other people, corruption perception, freedom to make life choices and healthy life expectancy directly and positively correlate with social well-being.Originality/valueThis study contributes to the previous literature in three ways. First, this paper provides fresh and recent data on social well-being. Second, the author introduced a multidimensional approach using a principal component analysis of the different social well-being factors to detect correlation between these indicators and to determine homogeneous clusters. Third, through these indicators, a country's leaders can formulate policies to enhance social well-being because it is closely linked to the improvement of the standard of living, good governance and therefore an increase in GDP.
Research on jet noise characteristics of aeroengine double S-bend nozzles with different cross sections
Authors: Shao Jiaru , Mo Xueping , Zheng Zijun , Yang Yu 
Published: December 2022

Purpose
This study aims to improve the survivability and maneuverability of the fighter,and study the stealth performance of fighter in the jet noise of aeroengine, it is of great significance to study the jet noise characteristics of double S-bend nozzles.

Design/methodology/approach
The multiparameter coupling and super-ellipse design methods are used to design the cross section of double S-bend nozzle. Taking unsteady flow information as the equivalent sound source, the noise signal at the far-field monitoring points were calculated with Ffowcs Williams–Hawkings (FW–H) method, and then, the sound source characteristics of the double S-bend nozzle are analyzed.

Findings
The results show that the internal flow of the S-bend nozzle with rectangular section is smoothed and the aerodynamic performance is better than super-ellipse section, the shear layer length of rectangular section is longer, the thickness is smaller and the mixing ability is stronger. The sound pressure level of the two S-bend nozzles decreases with the increase of the monitoring angle, and the sound pressure on the horizontal plane is greater than the vertical plane. In the direction of 40°–120°, the jet noise of rectangular nozzle is smaller, and the multiparameter coupled rectangular cross section structure is more applicable.

Practical implications
It is beneficial to reduce the jet noise of the engine tail nozzle and improve the stealth performance of the aircraft.

Originality/value
There is very little research on the jet noise characteristics of the double S-bend nozzle. The multiparameter coupling and the super-ellipse method are used to design the nozzle flow section to study the aerodynamic performance and jet noise characteristics of the double S-bend nozzle and to improve the acoustic stealth characteristics of the aircraft.
Wall laminar nanofluid jet flow and heat transfer
Authors: Khan Usafzai Waqar , Haq Rizwan Ul , Aly Emad H. 
Published: December 2022

Purpose
This work aims to investigates exact solutions of the classical Glauert’s laminar wall jet mass and heat transfer under wall suction, wall contraction or dilation, and two thermal transport boundary conditions; prescribed constant surface temperature and prescribed constant surface flux in nanofluidic environment.

Design/methodology/approach
The flow system arranged in terms of partial dif- ferential equations is non-dimensionalized with suitable dimensionless transformation variables, and this new set of equations is reduced into ordinary differential equations via a set of similarity transformations, where they are treated analytically for closed form solutions.

Findings
Exact solutions of nanofluid flow for velocity distributions, momentum flux, wall shear stress and heat transfer boundary layers for commonly studied nanoparticles; namely copper, alumina, silver, and titanium oxide are presented. The flow behavior of alumina and titanium oxide is identical, and a similar behavior is seen for copper and silver, making two pairs of identical traits. The mathematical expressions as well as visual analysis of wall shear drag and temperature gradient which are of practical interest are analyzed. It is shown that wall stretching or shrinking, wall transpiration and velocity slip together influences the jet flow mechanism and extends the original Glauert’s jet solutions. The exact solutions for the two temperature boundary layer conditions and temperature gradients are analyzed analytically. It is found that the effect of nanopar- ticles concentration on thermal boundary layer is intense, causing temperature uplift, whereas the wall transpiration causes a decrease in thermal layers.

Originality/value
The analysis carried out in nanofluid environment is genuinely new and unique, as our work generalizes the Glauert’s classical regular wall jet fluid problem.
Design creativity in Industry 4.0: Gravity Sketch and 3D printing in a Luminaire design project
Authors: Vo Hoa 
Published: December 2022

Purpose
This study aims to explore the impact of experiencing virtual reality (VR) and three-dimensional (3D) printing during the design process on the creativity of interior design students in a luminaire design project.

Design/methodology/approach
This study used the case-study approach within the context of a nine-week luminaire design project. Collected data included self-reported interest and engagement of students from a Qualtrics questionnaire and the ratings of their creativity via the Creative Product Semantic Scale (CPSS) with two judges.

Findings
Descriptive statistics from the Qualtrics questionnaire indicated an overall high level of student interest and engagement with the VR and 3D printing learning experience. Paired t-tests from CPSS ratings of the two judges showed a moderate increase in novelty and a significant increase in style with the introduction of VR and 3D printing technologies, respectively.

Research limitations/implications
Spearman’s correlations (rho) showed no statistical evidence for the relationships between CPSS ratings for creativity and students’ self-reported interest and engagement in VR and 3D printing learning experience.

Practical implications
Ample access time to VR technology and sufficient control over the 3D printing process are important for effective applications of Industry 4.0 technologies in organizations.

Social implications
This study dissected the confounding variables in its results as practical considerations for intergrading VR and 3D printing technologies for organizations in Industry 4.0.

Originality/value
This study acknowledged VR and 3D printing technologies as simulants for interest and engagement, which benefit creativity.
Local government officials’ susceptibility to corrupt behavior: some Indonesian evidence
Authors: Srirejeki Kiky , Putri Pretisila Kartika 
Published: December 2022

Purpose
This paper aims to investigate the factors affecting local government officials’ susceptibility to corrupt behavior among Indonesian local government officials.

Design/methodology/approach
This study uses a self-report survey and collected 449 questionnaires from 65 village government districts in Central Java province, Indonesia. This study uses a simple and partial correlation to measure the relationship between the susceptibility to corrupt behavior and the independent variables. Binary logistic regression was used to investigate which independent variables were best to explain the local government officials’ susceptibility to corrupt behavior.

Findings
The results show that the factors that best explain corrupt behavior’s susceptibility are the officials’ moral conviction to refrain from corrupt behavior, the perceived opportunity of corruption and the perceived benefit of engaging in such behavior. Further, this study finds an appealing crossover interaction between the perceived cost and social norms on corrupt behavior, such that when officials perceive the cost of engaging in corrupt behavior as low, they will rely more on social norms to decide whether to commit corrupt behavior.

Practical implications
This study provides actionable information for policy formulation. In particular, this study indicates that improvement of internal control can deter corrupt behavior. In addition, the findings of this study also suggest that changing the way we convey the message about corruption might be a promising intervention to mitigate corrupt behavior among government officials. More specifically, a more persuasive-positive-tone message that emphasizes the benefit of not engaging in corrupt behavior or that most people are against corruption can deter corrupt behavior.

Originality/value
The present study provides empirical evidence on the determinants of local government officials’ corrupt behavior from Indonesia’s perspective, which is currently limited.