bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mosaic-type trimeric RBD-based COVID-19 vaccine candidate induces potent neutralization against Omicron and other SARS-CoV-2 variants
Authors: Zhang Jing , Han Zi Bo , Liang Yu , Zhang Xue Feng , Jin Yu Qin , Du Li Fang , Shao Shuai , Wang Hui , Hou Jun Wei , Xu Ke , Lei Ze Hua , Liu Zhao Ming , Zhang Jin , Hou Ya Nan , Liu Ning , Shen Fu Jie , Wu Jin Juan , Zheng Xiang , Li Xin Yu , Li Xin , Huang Wei Jin , Wu Gui Zhen , Su Ji Guo , Li Qi Ming 
Published: March 2022

AbstractLarge-scale populations in the world have been vaccinated with COVID-19 vaccines, however, breakthrough infections of SARS-CoV-2 are still growing rapidly due to the emergence of immune-evasive variants, especially Omicron. It is urgent to develop effective broad-spectrum vaccines to better control the pandemic of these variants. Here, we present a mosaic-type trimeric form of spike receptor-binding domain (mos-tri-RBD) as a broad-spectrum vaccine candidate, which carries the key mutations from Omicron and other circulating variants. Tests in rats showed that the designed mos-tri-RBD, whether used alone or as a booster shot, elicited potent cross-neutralizing antibodies against not only Omicron but also other immune-evasive variants. Neutralizing antibody titers induced by mos-tri-RBD were substantially higher than those elicited by homo-tri-RBD (containing homologous RBDs from prototype strain) or the inactivated vaccine BBIBP-CorV. Our study indicates that mos-tri-RBD is highly immunogenic, which may serve as a broad-spectrum vaccine candidate in combating SARS-CoV-2 variants including Omicron.
CD169-mediated restrictive SARS-CoV-2 infection of macrophages induces pro-inflammatory responses
Authors: Jalloh Sallieu , Olejnik Judith , Berrigan Jacob , Nisa Annuurun , Suder Ellen L , Akiyama Hisashi , Lei Maohua , Tyagi Sanjay , Bushkin Yuri , Mühlberger Elke , Gummuluru Suryaram 
Published: March 2022

AbstractExacerbated and persistent innate immune response marked by pro-inflammatory cytokine expression is thought to be a major driver of chronic COVID-19 pathology. Although macrophages are not the primary target cells of SARS-CoV-2 infection in humans, viral RNA and antigens in activated monocytes and macrophages have been detected in post-mortem samples, and dysfunctional monocytes and macrophages have been hypothesized to contribute to a protracted hyper-inflammatory state in COVID-19 patients. In this study, we demonstrate that CD169, a myeloid cell specific I-type lectin, facilitated ACE2-independent SARS-CoV-2 fusion and entry in macrophages. CD169- mediated SARS-CoV-2 entry in macrophages resulted in expression of viral genomic and sub-genomic (sg) RNAs with minimal viral protein expression and no infectious viral particle release, suggesting a post-entry restriction of the SARS-CoV-2 replication cycle. Intriguingly this post-entry replication block was alleviated by exogenous ACE2 expression in macrophages. Restricted expression of viral gRNA and sgRNA in CD169+macrophages elicited a pro-inflammatory cytokine expression (TNFα, IL-6 and IL-1β) in a RIG-I, MDA-5 and MAVS-dependent manner, which was suppressed by remdesivir pre- treatment. These findings suggest thatde novoexpression of SARS-CoV-2 RNA in macrophages contributes to the pro-inflammatory cytokine signature and that blocking CD169-mediated ACE2 independent infection and subsequent activation of macrophages by viral RNA might alleviate COVID-19-associated hyperinflammatory response.Author SummaryOver-exuberant production of pro-inflammatory cytokine expression by macrophages has been hypothesized to contribute to severity of COVID-19 disease. Molecular mechanisms that contribute to macrophage-intrinsic immune activation during SARS- CoV-2 infection are not fully understood. Here we show that CD169, a macrophage- specific sialic-acid binding lectin, facilitates abortive SARS-CoV-2 infection of macrophages that results in innate immune sensing of viral replication intermediates and production of proinflammatory responses. We identify an ACE2-independent, CD169- mediated endosomal viral entry mechanism that results in cytoplasmic delivery of viral capsids and initiation of virus replication, but absence of infectious viral production. Restricted viral replication in CD169+macrophages and detection of viral genomic and sub-genomic RNAs by cytoplasmic RIG-I-like receptor family members, RIG-I and MDA5, and initiation of downstream signaling via the adaptor protein MAVS, was required for innate immune activation. These studies uncover mechanisms important for initiation of innate immune sensing of SARS-CoV-2 infection in macrophages, persistent activation of which might contribute to severe COVID-19 pathophysiology.
Sex differences in allometry for mouse phenotypic traits indicate that females are not scaled males
Authors: Wilson Laura A. B. , Zajitschek Susanne R. K. , Lagisz Malgorzata , Mason Jeremy , Haselimashhadi Hamed , Nakagawa Shinichi 
Published: March 2022

AbstractSex differences in the lifetime risk and expression of disease are well-known. Preclinical research targeted at improving treatment, increasing health span, and reducing the financial burden of health care, has mostly been conducted on male animals and cells. The extent to which sex differences in phenotypic traits are explained by sex differences in body weight remains unclear. We quantify sex differences in the allometric relationship between trait value and body weight for 375 phenotypic traits in male and female mice, recorded in >2.1 million measurements from the International Mouse Phenotyping Consortium. We find sex differences in allometric parameters (slope, intercept, residual SD) are common (76% traits). Body weight differences do not explain all sex differences in trait values but scaling by weight may be useful for some traits. Our results support a trait-specific patterning of sex differences in phenotypic traits, promoting case-specific approaches to drug dosage scaled by body weight.
DTSyn: a dual-transformer-based neural network to predict synergistic drug combinations
Authors: Hu Jing , Gao Jie , Fang Xiaomin , Liu Zijing , Wang Fan , Huang Weili , wu Hua , Zhao Guodong 
Published: March 2022

AbstractDrug combination therapies are superior to monotherapy for cancer treatment in many ways when addressing tumor heterogeneity issue. For wet-lab experiment, screening out novel synergistic drug pairs is challenging due to the enormous searching space of possible drug pairs. Thus, computational methods have been developed to predict drug pairs with potential synergistic function. Notwithstanding the success of current models, the power of generalization to other datasets as wells as understanding of mechanism for chemical-chemical interaction or chemical-sample interaction are lack of study, hindering current algorithms from real application. In this paper, we proposed a deep neural model termed DTSyn (Dual Transformer model for drug pair Synergy prediction) based on multi-head attention mechanism to identify novel drug combinations. We designed a fine-granularity transformer for capturing chemical substructure-gene and gene-gene associations and a coarse-granularity transformer for extracting chemical-chemical and chemical-cell line interactions. DTSyn achieves highest Receiver operating characteristic area under curve (ROC AUC) of 0.73, 0.78. 0.82 and 0.81 on four different cross validation tasks, outperforming all competing methods. Further, DTSyn achieved best True Positive Rate (TPR) over five independent datasets. The ablation study showed that both transformer blocks contributed to the performance of DTSyn. In addition, DTSyn can extract interactions among chemicals and cell lines, which may represent the mechanisms of drug action. Thus, we envision our model a valuable tool to prioritize synergistic drug pairs by utilizing chemicals and transcriptome data.
Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein/dynactin is essential for cleavage furrow formation during anaphase
Authors: Sana Shrividya , Rajeevan Ashwathi , Kotak Sachin 
Published: March 2022

AbstractIn animal cells, spindle elongation during anaphase is temporally coupled with cleavage furrow formation. Spindle elongation during anaphase is regulated by NuMA/dynein/dynactin complexes that occupy the polar region of the cell membrane and are excluded from the equatorial membrane. How NuMA/dynein/dynactin are excluded from the equatorial membrane and the biological significance of this exclusion remains unknown. Here, we show that the centralspindlin (Cyk4/Mklp1) and its interacting partner RhoGEF Ect2 are required for NuMA/dynein/dynactin exclusion from the equatorial cell membrane. The Ect2-based (Ect2/Cyk4/Mklp1) and NuMA-based (NuMA/dynein/dynactin) complexes occupy mutually exclusive membrane surfaces during anaphase. The equatorial membrane enrichment of Ect2-based complexes is essential for NuMA/dynein/dynactin exclusion and proper spindle elongation. Conversely, NuMA-based complexes at the polar region of the cell membrane ensure spatially confined localization of Ect2-based complexes and thus RhoA. Overall, our work establishes that membrane compartmentalization of NuMA-based and Ect2-based complexes at the two distinct cell surfaces restricts dynein/dynactin and RhoA for coordinating spindle elongation with cleavage furrow formation.
Structural basis of actin filament assembly and aging
Authors: Oosterheert Wout , Klink Björn U , Belyy Alexander , Pospich Sabrina , Raunser Stefan 
Published: March 2022

AbstractThe dynamic turnover of actin filaments (F-actin) controls cellular motility in eukaryotes and is coupled to changes in the F-actin nucleotide state. It remains unclear how F-actin hydrolyzes ATP and subsequently undergoes subtle conformational rearrangements that ultimately lead to filament depolymerization by actin-binding proteins. Here, we present cryo-EM structures of F-actin in all nucleotide states, polymerized in the presence of Mg2+ or Ca2+, at resolutions (∼2.2 Å) that allow for the visualization of hundreds of water molecules. The structures reveal that the G- to F-actin transition induces the relocation of water molecules in the nucleotide binding pocket, activating one of them for the nucleophilic attack of ATP. Unexpectedly, the back door for the subsequent release of inorganic phosphate (Pi) is closed in all structures, indicating that the F-actin conformation that allows for Pi release occurs transiently. The small changes in the nucleotide-binding pocket after ATP hydrolysis and Pi release are sensed by a key amino acid, amplified and transmitted to the filament periphery. Furthermore, differences in the positions of waters in the nucleotide binding pocket explain why Ca2+-actin exhibits slower polymerization rates than Mg2+-actin. Our work elucidates the solvent-driven rearrangements that govern actin filament assembly and aging and lays the foundation for the rational design of drugs and small molecules for imaging and therapeutic applications.
Mechano-redox control of Mac-1 de-adhesion from ICAM-1 by protein disulfide isomerase promotes directional movement of neutrophils under flow
Authors: Dupuy Alexander , Santamaría Camilo Aponte , Yeheskel Adva , Gräter Frauke , Hogg Philip J. , Passam Freda H. , Chiu Joyce 
Published: March 2022

AbstractMacrophage-1 antigen or Mac-1 (CD11b/CD18, αMβ2) is a leukocyte integrin essential for firm adhesion of neutrophils, lymphocytes and monocytes against flow when recruited to the endothelium. To migrate to the site of inflammation, leukocytes require coordinated adhesion and de-adhesion for directional movement. The vascular thiol isomerase, protein disulfide isomerase (PDI), was found by fluorescence microscopy to colocalize with high affinity Mac-1 at the trailing edge of stimulated neutrophils when adhered to ICAM-1 under fluid shear. From differential cysteine alkylation and mass spectrometry studies, PDI cleaves two allosteric disulfide bonds, C169-C176 and C224-C264, in the βI domain of the β2 subunit, and in mutagenesis and cell transfection studies, cleavage of the C224-C264 disulfide bond was shown to selectively control Mac-1 dis-engagement from ICAM-1 under fluid shear. Molecular dynamics simulations and binding of conformation-specific antibodies reveal that cleavage of the C224-C264 bond induces conformational change and mechanical stress in the βI domain that allosterically alters exposure of an αI domain epitope and shifts Mac-1 to a lower affinity state. From studies of neutrophil adherence to ICAM-1 under fluid shear, these molecular events promote neutrophil motility in the direction of flow at high shear stress. In summary, shear-dependent PDI cleavage of neutrophil Mac-1 C224-C264 disulfide bond triggers Mac-1 de-adherence from ICAM-1 at the trailing edge of the cell and enables directional movement of neutrophils during inflammation.
Phylogenomics Illuminates the Evolutionary History of Wild Silkmoths in Space and Time (Lepidoptera: Saturniidae)
Authors: Rougerie Rodolphe , Cruaud Astrid , Arnal Pierre , Ballesteros-Mejia Liliana , Condamine Fabien L. , Decaëns Thibaud , Elias Marianne , Gey Delphine , Hebert Paul D. N. , Kitching Ian J. , Lavergne Sébastien , Lopez-Vaamonde Carlos , Murienne Jérôme , Cuenot Yves , Nidelet Sabine , Rasplus Jean-Yves 
Published: March 2022

AbstractWild silkmoths (Saturniidae) are one of the most emblematic and most studied families of moths. Yet, the absence of a robust phylogenetic framework based on a comprehensive taxonomic sampling impedes our understanding of their evolutionary history. We analyzed 1,024 ultraconserved elements (UCEs) and their flanking regions to infer the relationships among 338 species of Saturniidae representing all described subfamilies, tribes, and genera. We investigated systematic biases in genomic data and performed dating and historical biogeographic analyses to reconstruct the evolutionary history of wild silkmoths in space and time. Using Gene Genealogy Interrogation, we showed that saturation of nucleotide sequence data blurred our understanding of early divergences and first biogeographic events. Our analyses support a Neotropical origin of saturniids, shortly after the Cretaceous-Paleogene extinction event (ca64.0 [stem] - 52.0 [crown] Ma), and two independent colonization events of the Old World during the Eocene, presumably through the Bering Land Bridge. Early divergences strongly shaped the distribution of extant subfamilies as they showed very limited mobility across biogeographical regions, except for Saturniinae, a subfamily now present on all continents but Antarctica. Overall, our results provide a framework for in-depth investigations into the spatial and temporal dynamics of all saturniid lineages and for the integration of their evolutionary history into further global studies of biodiversity and conservation. Rather unexpectedly for a taxonomically well-known family such as Saturniidae, the proper alignment of taxonomic divisions and ranks with our phylogenetic results leads us to propose substantial rearrangements of the family classification, including the description of one new subfamily and two new tribes.
Reanalysis of double EphA3 knockin maps in mouse suggests that stochasticity in topographic map formation acts within the retinal cell population rather than between competing mechanisms at the colliculus
Authors: Willshaw David J , Gale Nicholas M 
Published: March 2022

AbstractIt has been suggested that stochasticity can act in the formation of topographically ordered maps in the visual system through the opposing chemoaffinity and neural activity forces acting on the innervating nerve fibres being held in an unstable equilibrium. Evidence comes from the Islet2-EphA3 knockin mouse, in which approximately 50% of the retinal ganglion cells, distributed across the retina, acquire the EphA3 receptor, thus having an enhanced density of EphA which specifies retinotopic order along one axis of the retinocollicular map. Sampling EphA3 knockin maps in heterozygotes at different positions along the mediolateral extent of the colliculus had found single 1D maps (as in wild-types), double maps (as in homozygous knockins) or both single and double maps. We constructed full 2D maps from the same dataset. We found either single maps or maps where the visual field projects rostrally, with a part-projection more caudally to form a double map, the extent and location of this duplication varying considerably. Contrary to previous analyses, there was no strict demarcation between heterozygous and homozygous maps. These maps were replicated in a computational model where, as the level of EphA3 was increased, there was a smooth transition from single to double maps. Our results suggest that the diversity in these retinotopic maps has its origin in a variability over the retina in the effective amount of EphA3, such as through variability in gene expression or the proportion of EphA3+ retinal ganglion cells, rather than the result of competing mechanisms acting at the colliculus.Significance statementAnalysis by others of visuocollicular maps in EphA3 knockin mice indicated stochasticity in the development of nerve connections. Here a proportion of the retinal ganglion cells have the chemoaffinity ligand EphA3. Sampling the heterozygous map revealed either a single map (as in wildtypes), a double map (as in homozygotes) or a mixture, suggesting an unstable balance between competing chemoaffinity and neural activity forces. We constructed full 2D maps from the same dataset. We found a diversity of double maps in both genotypes, with no demarcation between heterozygote and homozygote, replicated in a computational model where the level of EphA3 varies between animals. We suggest that any stochasticity acts at the level of the retina rather than downstream at the colliculus.
Lack of universal mutational biases in a fungal phylum
Authors: Zheng Qianhui , Steenwyk Jacob L. , Rokas Antonis 
Published: March 2022

AbstractMutations fuel the diversity of life forms on earth through changes of nucleotides in DNA sequence. Patterns of mutations are biased; for example, mutational biases toward adenine and thymine have been previously noted in bacteria and biases for transitions over transversions are observed in diverse groups of organisms. However, the mutational biases in fungi, whose genomes vary widely in their GC content, remain poorly understood. Here, we characterized patterns of single nucleotide polymorphisms among 537 strains from 30 species and four classes from Ascomycota, the most species-rich fungal phylum. We found that mutational biases vary across Ascomycota; for example, some species in the class Saccharomycetes, in particular the bipolar budding yeast Hanseniaspora uvarum and the emerging pathogen Candida auris, show strong mutational bias toward A|T substitutions whereas the black mold Stachybotrys chartarum in the class Sordariomycetes shows substantial mutational bias toward G|C substitutions. Examination of GC content and GC equilibrium content, a measure that represents the GC content under selective neutrality and accounts for rates of G|C > A|T and A|T > G|C substitutions, revealed that fungal species vary in how their genome nucleotide composition is affected by neutral processes, mutational biases, and external evolutionary forces, such as selection. Whereas genome nucleotide composition is consistent with neutral expectations and is mainly driven by mutational bias in some species (e.g., Zymoseptoria tritici), the composition of others is influenced by both mutational bias and selection (e.g., H. uvarum and S. chartarum). These results highlight the variation of patterns of mutations across a fungal phylum and suggest that both neutral and selective processes shape the nucleotide composition of fungal genomes.
Effects of low intensity pulsed ultrasound stimulation on metabolic lipolysis of adipocytes
Authors: Kim Sangnam , Yoon Sangpil 
Published: March 2022

ABSTRACTObesity is closely related to several metabolic diseases along with abnormal increase in fat cells. Reducing size and number of fat cells, a procedure known as lipolysis, may be used to prevent obesity as a potential therapy, which also requires fundamental understanding of the mechanisms of lipolysis at molecular level upon different types of stimulations. Here, we used low intensity pulsed ultrasound (LIPUS) stimulation to investigate underlying mechanisms of the activation of lipolysis and autophagy related genes and signaling pathways of adipocytes differentiated from 3T3-L1 cells. LIPUS with the center frequency of 2 MHz was applied for 10 minutes per day for three days. After LIPUS stimulation, quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blot were used to determine the regulation of lipolytic factors such as adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and monoacylglycerol lipase (MGL). At RNA level, all three factors were upregulated while only MGL was upregulated at protein level, which presents slightly different activation pattern of lipolysis compared to widely used chemical stimulation. These results demonstrate that LIPUS stimulation can promote the lipolytic capacity of adipocytes in the differentiated state. The differences between transcriptional genes and metabolites were analyzed by transcript analysis and metabolomic profiling experiments. Cellular RNA-sequencing (RNA-Seq) showed an increase in lipolysis and immune-related genes and autophagy related genes after LIPUS stimulation. This study may provide an important experimental basis for the clinical applications and a fundamental understanding of the mechanisms of lipolysis using LIPUS stimulation.
Boquila: NGS read simulator to eliminate read nucleotide bias in sequence analysis
Authors: Akkose Umit , Adebali Ogun 
Published: March 2022

AbstractSequence content is heterogeneous throughout genomes. Therefore, Genome-wide NGS reads biased towards specific nucleotide profiles are affected by the genome-wide heterogeneous nucleotide distribution. Boquila generates sequences that mimic the nucleotide profile of true reads, which can be used to correct the nucleotide-based bias of genome-wide distribution of NGS reads. Boquila can be configured to generate reads from only specified regions of the reference genome. It also allows the use of input DNA sequencing to correct the bias due to the copy number variations in the genome. Boquila uses standard file formats for input and output data, and it can be easily integrated into any workflow for high-throughput sequencing applications.