bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual Specificity Phosphatase 7 Drives the Formation of Cardiac Mesoderm in Mouse Embryonic Stem Cells
Authors: Sladeček Stanislava , Radaszkiewicz Katarzyna Anna , Bőhmová Martina , Gybeľ Tomáš , Radaszkiewicz Tomasz Witold , Pacherník Jiří 
Published: February 2022

Abstract
Background: Dual specificity phosphatase 7 (DUSP7) is a protein belonging to a broad group of phosphatases that can dephosphorylate phosphoserine/phosphothreonine as well as phosphotyrosine residues within the same substrate. DUSP7 has been linked to the negative regulation of mitogen activated protein kinases (MAPK), and in particular to the regulation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). MAPKs play an important role in embryonic development, where their duration, magnitude, and spatiotemporal activity must be strictly controlled by other proteins, among others by DUSPs.Methods: In this study, we focused on the effect of DUSP7 depletion on the in vitro differentiation of mouse embryonic stem (ES) cells. Undifferentiated stem cells as well as cells at various days of in vitro differentiation protocol were used for analysis using mainly qPCR, western blotting and immunohistochemistry. Data are expressed as mean ± standard deviation (SD). Statistical analysis was assessed by t-test and by one- or two-way ANOVA, and by Bonferroni’s Multiple Comparison post hoc test. Values of P < 0.05 were considered to be statistically significant (∗ p < 0.05).Results: We showed that even though DUSP7 knock-out ES cells do retain some of their basic characteristics, when it comes to differentiation, they preferentially differentiate towards neural cells, while the formation of early cardiac mesoderm is repressed.Conclusions: Our data indicate that DUSP7 is necessary for the correct formation of neuroectoderm and cardiac mesoderm during the in vitro differentiation of ES cells.
Electrically Tunable Plasmonic Absorber Based On Cu-ITO Subwavelength Grating On SOI At Telecom Wavelength
Authors: Pandey Suresh Kumar , Rajput Swati , Kaushik Vishal , Babu Prem , Mishra Rahul Dev , Kumar Mukesh 
Published: February 2022

Abstract
An electrically controlled optical absorption is numerically proposed in a plasmonic waveguide on silicon-oninsulator (SOI) consisting of copper-Indium tin oxide (ITO) based subwavelength grating at 1.55 µm wavelength. The Cu-ITO subwavelength grating in form of a discontinuous Cu layer filled with ITO together with electrically tunable permittivity of ITO provides us with a tunable absorption and efficient guidance of plasmonic mode. An n-type ITO is used which exhibits a significant change in the carrier concentration with the applied voltage resulting in a change in optical absorption at a telecom wavelength of 1.55 µm. We numerically observe maximum tuning in absorption at a grating period of 600 nm and a duty cycle of 50%. The proposed device shows a smaller effective mode area of Am= 0.01421 /µm2 and a plasmonic confinement factor of 34.67%. The device is reported to have an extinction ratio of 10.28 dB for a 100 µm long device at a low voltage of 6V.
Optimized Method of Lines for non-linear waveguide problems
Authors: Spiller Waldemar 
Published: February 2022

Abstract
The Method of Lines is a semi-analytical versatile tool for the solution of partial differential equations. For the analysis of spatial complex linear waveguide structures, this method is combined with impedance/admittance and field transformation, as well as with finite differences. This paper extends this approach to the treatment of structures with non-linear dielectric materials. The non-linear generalized transmission line equations are derived. An iterative algorithm based on the impedance/admittance transformation with the field transformation obtains efficient and self-consistent solutions. The specific limiting factors for the algorithm and how to overcome them were investigated. A bidirectional, spatially, and temporally periodic energy exchange between the harmonics were found. For demonstration, a stripe waveguide with the non-linear core is considered. The Kerr non-linearity was investigated, though the general case is treatable. The approach can be used for any spatial structure, including, for instance, photonic crystal waveguides and metamaterials.
Enrichment of CD44+/CD24+ cells predicts chemoresistance in luminal breast cancer
Authors: Tang Peng , Wu Jin , Ma Qinghua , Liu Wei , Wang Minghao , Yan Yuzhao , Hu Ying , Zhong Ling , Chen Qingqiu 
Published: February 2022

Abstract
Purpose: Chemoresistant cells are pre-existing and adaptively selected by chemotherapy. The biomarkers of chemoresistant cells in luminal breast cancer remain unknown. The expression of CD44 and CD24 are associated with breast cancer progression. This study aimed to explore the proportion change of differently expressed CD44/CD24 tumor cells during the induction of chemoresistance.Methods: Tumor specimens of patients with luminal breast cancer resistant and sensitive to chemotherapy drugs regimens were collected. The proportion of CD44+/−CD24+/− tumor cells in the two groups of samples was compared by immunofluorescence staining. MCF-7 and T47D variants resistant to chemotherapy drugs were induced by pulse selection of the parental cells with the IC90 value of the two drugs for 7 weeks. Changes in the proportion of CD44+/−CD24+/− cells during the induction of chemoresistance were observed and compared using flow cytometry.Results: The proportion of CD44+CD24+ cells in the tumor tissues of chemoresistant patients was significantly higher than chemosensitive patients. At the endpoint of chemoresistance induction, the proportions of CD44+CD24+ cells reached 86.27%, 84.41%, 83.03%, and 85.12% in MCF-7/EPI, MCF-7/DOC, T47D/EPI, and T47D/DOC, respectively. The proportion of CD44+CD24− cells increased briefly and then decreased to nearly the pre-induction level (3.01%). The proportion of CD44−CD24− and CD44−CD24+ cells in the four chemoresistant variants was less than 5% at the endpoint of chemoresistance induction. Conclusions: The enrichment of CD44+CD24+ cells predicts chemoresistance in luminal breast cancer. CD44+CD24+ is a biomarker for chemoresistant luminal breast cancer cells.
MRI based navigated cryosurgery of extra-abdominal desmoid tumors: A proof of concept study
Authors: Efrima Ben , Segal Ortal , Benady Amit , Pickholz Eliana , Drukman Ido , Dadia Solomon , Rath Ehud , Albagli Assaf 
Published: February 2022

Abstract
Background: Computer-guided MRI navigation for soft tissue tumors remains a challenge due to lack of a fixed landmark for registration. Extra-abdominal Desmoid Tumors (EDTs) are soft tissue tumors for which image-guided percutaneous cryosurgery (CRA) has emerged as a valid treatment modality. Successful CRA relies on precise intraoperative image guidance. This study, present a novel approach of using skin fiducial markers to overcome this challenge and navigate insertion of MRI-based CRA needles as a treatment for symptomatic or progressive EDTs. Methods: In this retrospective study, conducted between 2018-2020, eleven patients at a single center with symptomatic or progressive EDTs were treated with CRA using intraoperative MRI navigation. 15 cryoablation procedures were performed, each adhering to a personalized pre-operative plan. We compared the total tumor size, necrotic and viable portions pre- and post-operation, and the SF-36 questionnaire evaluating subjective health.Results: All CRAs demonstrated 100% adherence to the pre-determined plan. Overall, tumor size decreased significantly (p-value=0.02). Four patients required additional CRAs. Only one patient’s tumor did not reduce in size. There was a significant reduction in viable tissue, (p-value=0.03), but not in necrotic tissue (p-value=0.66). According to the SIR adverse event classification guidelines, one participant experienced muscle necrosis, a mild complication. Both the physical and mental scores improved significantly (p-value (physical)=0.002; p-value (mental)<0.000).Conclusion: These findings demonstrate the feasibility and efficacy of performing percutaneous cryosurgery using skin fiducial marker registration and MRI-computed navigation to safely treat EDTs. This navigation approach may be implemented in other soft tissue procedures requiring precision navigated surgery. Trial Registration: Retrospectively registered.
Elevation of α-1,3 fucosylation promotes binding ability of TNFR1 to TNF-α and contributes to osteoarthritic cartilage destruction and apoptosis
Authors: Yu Hanjie , Li Mingxiu , Wen Xiaodong , Yang Jie , Liang Xiaojun , Li Xia , Bao Xiaojuan , Shu Jian , Ren Xiameng , Chen Wentian , Li Zheng , Li Yi 
Published: February 2022

Abstract
Background: Osteoarthritis (OA) is characterized by ECM degradation in chondrocytes and inflammation of the synovial membrane. Fucosylation is a main type of glycosylation, and is involved in a variety of pathological processes, including inflammation and cancer. However, whether fucosylation impacts the OA process is unknown. Methods: Total proteins were extracted from cartilage samples obtained from patients with OA (n = 11), OA rabbit models at different time points (n = 12). The OA-associated abnormal glycopatterns were evaluated by lectin microarray and lectin blot. Expression of fucosyltransferases involved in the synthesis of α-1,3 fucosylation were assessed by semi-qPCR. The α-1,3 fucosylation mediated by FUT10 was interrupted by transfection of siRNA; and the effect of α-1,3 fucosylation on OA-associated events was assessed. The TNFR1 antibody microarray was fabricated to investigate the effect of α-1,3 fucosylation on the binding ability of TNFR1 to TNF-α.Results: Elevated α-1,3 fucosylation is observed in cartilage from OA patients, rabbit models and chondrocytes induced by TNF-α (fold change> 2, p< 0.01). The overexpression of FUT10 contributes to this alteration. Downregulation of FUT10 impaired the binding ability of TNFR1 to TNF-α, impeded activation of the NF-κB and P38/JNK-MAPK pathways and eventually retarded ECM degradation, senescence and apoptosis of chondrocytes induced by TNF-α. Conclusion: The elevation of α-1,3 fucosylation is not only a characteristic of OA but also impacts the OA process. Our work provides a new positive feedback loop of “inflammation conditions/TNF-α/FUT10/α-1,3 fucosylation of TNFR1/NF-κB and P38/JNK-MAPK pathways/proinflammatory processes” that contributes to ECM degradation and apoptosis of chondrocytes.
The Comparative Effects of Schwann Cells and Wharton's Jelly Mesenchymal Stem Cells on the AIM2 Inflammasome Activity in Animal Model of Spinal Cord Injury
Authors: Kharazinejad Ebrahim , Hassanzadeh Gholamreza , Mortezaee Keywan , Yousefi Behpour , Majidpoor Jamal , Golchini Ehsan , Nooshabad Vajihe Taghdiri , Mousavi Mahboubeh 
Published: February 2022

Abstract
Inflammasome activation and the corresponding release of pro-inflammatory cytokines are responsible for sensory/motor deficits following spinal cord injury (SCI). Schwann cells (SCs) and Wharton’s jelly mesenchymal stem cells (WJ-MSCs) represent immunomodulatory activities. Here, we aimed at comparing the efficacy of the two cell sources on absent in melanoma 2 (AIM2) inflammasome complex in SCI rats. Basso, Beattie, Bresnahan (BBB) test, Nissl and Luxol fast blue (LFB) staining were performed to assess locomotor function, neuronal survival and myelination, respectively. Gene and protein expressions of inflammasome components including AIM2, ASC, caspase-1, interleukin-1β (IL-1β) and IL-18 were analyzed by real-time polymerase chain reaction (RT-PCR), western blotting and enzyme-linked immunosorbent assay (ELISA). Expressions of the whole evaluated factors were declined in both gene and protein levels after SC or WJ-MSC treatment, which were more conspicuous for the SCs group (P < 0.05). Similarly, SCs increased the rates of neuronal survival and myelination. Furthermore, cell administration caused improvement in motor recovery in both treatment groups (P < 0.05). Although the outcomes were not significant, these effects were more notable in the SC-treated animals. From the findings it could be concluded that SC therapy is more efficient for targeting AIM2 inflammasome activation and related inflammatory circuit in SCI experiments than that for WJ-MSCs.
Evaluation of Demographic, Clinical, and Etiological Data of Patients Admitted to Cardiology Clinics And Diagnosed with Left Ventricular Hypertrophy (LVH) on Electrocardiography And/Or Echocardiography: LVH-TR
Authors: Kis Mehmet , Dogan Yasemin , Yildirim Abdullah , Guzel Tuncay , Bekar Lutfu , Akhan Onur , Dogdus Mustafa , Harbalıoglu Hazar , Karabulut Dilay , Soydan Elton , Oztas Selvi , Oz Ahmet , Gok Gulay , Kolak Zeynep , Aksit Ercan , Sarica Suleyman Anil , Bayrak Murat , Ekici Berkay , Birdal Oguzhan , Uguz Berat , Gitmez Mesut , Caglar Nihan , Berk Gul Ilayda , Oguz Mustafa , Calik Ali Nazmi , Kilic Salih , Zoghi Mehdi , Ergene Asim Oktay 
Published: February 2022

Abstract
Purpose: Left ventricular hypertrophy (LVH) is a common condition in the population and potentially modifiable cardiovascular (CV) risk factor often overlooked in clinical practice. Early diagnosis and treatment-related regression of LVH reduces adverse CV events and improves survival. For this reason, we planned to LVH-TR trial to determine the etiological causes of LVH, the symptoms that patients present with, and the effects of demographic characteristics of patients on LVH.Methods: Our study is a multicenter, national, observational study and included 886 patients who applied to the cardiology clinics in 23 centers between February 2020 and August 2021. In the initial evaluation, the Fabry and amyloidosis algorithm was followed in patients whose traditional etiologic cause(s) could not be identified (LVH of unknown origin). Results: The mean age of the patients was 58.92 years, and 60.3% of them were male. More than 90% of the patients were NYHA class I & II patients. The most common etiological causes of LVH in our study were hypertension with a rate of 69.7%, heart valve disease with 10.2%, and hypertrophic cardiomyopathy with 9.2%. Athlete's heart was detected in 8 patients, LV non-compaction was detected in 4 patients.The rate of LVH of unknown cause was 18.8%. Fabry disease was suspected in 143 patients, and Amilodose disease was suspected in 16 patients. There were 43 (4.85%) patients with low α-Galactosidase A enzyme levels in patients who requested enzyme testing. GLA gene mutation analysis was positive in 1.58% of all patients, and these patients were diagnosed with Fabry, and 15 (1.69%) patients were diagnosed with amyloidosis by endomyocardial biopsy method.Conclusion: In the etiology of left ventricular hypertrophy, the rate of LVH of unknown cause was high. Fabry Disease and Amyloidosis should be considered primarily in this patient group. Early diagnosis of the disease by following the schemes leading to Fabry and Amyloidosis was essential in starting treatment before the progression of the disease.
Prognostic Implications of PPL Expression in Ovarian Cancer
Authors: Hua Tian , Zhao Bei-bei , Fan Shao-bei , Zhao Cai-fen , Kong Yun-hong , Tian Rui-qing , Zhang Bao-ying 
Published: February 2022

Abstract
Periplakin (PPL) is a main member in plakin family, which plays important role in cellular adhesion complexes supporting and cytoskeletal integrity supplying. PPL was reported to be a potential biomarker candidate for several types of cancers. However, the biological functions and underlying mechanisms of PPL in ovarian cancer (OV) remain unclear. In the present study, we used GEPIA2, Human Protein Atlas, Oncomine, LinkedOmics, Kaplan-Meier Plotter, String, CytoHubba plug-in and TIMER to determine the associations among PPL expression, prognosis, and immune cell infiltration in OV. RT-qPCR and IHC analysis were conducted to validated the role of PPL in an independent OV cohort. Compared with the ovaries tissues from multiple datasets, levels of PPL mRNA and protein expression were obviously higher in OV tumors(P＜0.05), and a poor survival in OV was found to be strongly correlated with high PPL expression (P<0.05). Moreover, the results were further validated by RT-qPCR and IHC analysis in an independent OV cohort. A gene-clinical nomogram was constructed, including PPL expression and clinical characterization in TCGA. Functional network analysis suggested that PPL regulates important pathways like Wnt signaling pathway, MAPK signaling pathway. Ten hub genes (LAMC2, PXN, LAMA3, LAMB3, LAMA5, ITGA3, TLN1, ACTN4, ACTN1, and ITGB4) were found to be positively associated with PPL. PPL expression was negatively correlated with infiltrating levels of CD4+ T cell macrophages, neutrophils, and dendritic cells. In conclusion, PPL may be an unfavorable prognostic biomarker candidate in OV, which was also correlated with immune infiltrating and might function in immunotherapy response.
Fe, Zn &amp; Protein content in grain, per se performance, heterosis, combining ability of grain yield in bread wheat (Triticum aestivum) under normal &amp; late sowing condition.
Authors: Chaudhari Gita R. , Patel D. A. , Parmar D. J. Parmar , Patel K. C. 
Published: February 2022

Abstract
The aim of this study was to understand the inheritance of grain iron (Fe), zinc (Zn) and protein concentrations in bread wheat (Triticum aestivum L.) under normal & late sown condition. The half diallel cross were made, using ten parents. The crosses and parents were evaluated in replicated trials for two conditions and to assess the possibility of exploiting heterosis to improve these micronutrients. The perusal of results revealed that hybrid GW 451 × GW 173 was consistent for both better parent (BH) and standard heterotic effects (SH) in all the environments. In both the sowing condition, General Combining Ability (GCA) effects of parents and Specific Combining Ability (SCA) effects of hybrids were observed to be contrasting for the micronutrient & protein content. However, s2A/s2D greater than unity confirmed the preponderance of additive gene action for only protein content and GW 173 was identified as good general combiners for these characters under both environments.
Impacts of Anthropogenic Disturbance on Vegetation Structure of El-Maktala Coastal land, Egypt
Authors: Shawky Ramadan , Kherishy Ahmed 
Published: February 2022

Abstract
The anthropogenic disturbance on vegetation in El-Maktala coastal land, an unprotected area in the north western coast of the Egypt. That has recently been subjected to blatant encroachments of vegetation, were studied. The most important of these threats is the construction of the road and grazing, 27 species are reported in this study; the most represented plant families are Poaceae (18.5%) and Asteraceae (14.8%). Perennial, chamaephyte and Mediterranean species were recorded in the highest percentage. Canonical correspondence analysis showed that silt, clay, sand contents, PH, EC and CaCO3 content are the factors that have the highest effect on vegetation distribution in the studied stands. The diversity indices in the studied area was moderate. According to Sorenson’s coefficient, these communities have quite a bit of overlap or similarity. The Vegetation Quality Index indicated that coastal land is highly sensitive to desertification, as decrease in plant cover. Recently, the violation and destruction of wildlife have increased, therefore, preserving it along with general biodiversity has become an urgent necessity.
Association of Myostatin Gene Polymorphisms with Strength and Muscle Mass in Athletes: a Systematic Review and Meta-Analysis of the MSTN rs1805086 Mutation
Authors: Olegovich Aksenov Maxim , Kruszewski Marek 
Published: February 2022

Abstract
Introduction.
Variant (rs1805086, c.458A>G, p.Lys(K)153Arg(R), (K153R) of the myostatin gene (MSTN) has been associated with a skeletal muscle phenotype (hypertrophic response in muscles due to strength training). However, there are not enough reliable data to demonstrate whether the K and R allelic variants of MSTN rs1805086 are valid genetic factors that can affect the strength phenotype of athletes’ skeletal muscles.
Purpose.
To conduct a systematic review and meta-analysis of the association of MSTN rs1805086 polymorphism with the strength phenotype of athletes.
Materials and methods.
This study analyzed 71 research articles on myostatin and performed a meta-analysis of MSTN K153R rs1805086 polymorphism in strength-oriented athletes and control (non-athletes) group.
Results.
It was found that athletes in the strength-oriented athletes group had a higher frequency of the R minor allele than that in the control group (OR = 2.02, P = 0.05).
Conclusion.
Thus, the obtained results convincingly demonstrate that there is an association between the studied polymorphism and strength phenotype of athletes; therefore, further studies on this association are scientifically warranted.
Depression and Bipolar Disorder Subtypes Differ in their Genetic Correlations with Biological Rhythms
Authors: Sirignano Lea , Streit Fabian , Frank Josef , Zillich Lea , Witt Stephanie , Rietschel Marcella , Foo Jerome 
Published: February 2022

Abstract
Major Depression and Bipolar Disorder Type I (BIP-I) and Type II (BIP-II), are mood disorders characterized by the occurrence of depressed, manic, and hypomanic episodes in which specific changes of physical activity, circadian rhythm, and sleep are observed. While it is known that genetic factors contribute to variation in both mood disorders and these biological rhythms, it is an open question to what extent there is an overlap between their underlying genetics.Here, we used data from genome-wide association studies to examine the genetic relationship between mood disorders and biological rhythms. Linkage Disequilibrium Score Regression was applied to test the genetic correlation of depression, BIP-I, and BIP-II with physical activity (overall physical activity, moderate activity, sedentary behaviour), circadian rhythm (relative amplitude), and sleep features (sleep duration, daytime sleepiness). Furthermore, we compared depression, BIP-I, and BIP-II in their correlations with biological rhythms to discover commonalities and differences. Depression was negatively correlated with overall physical activity and positively with sedentary behaviour, while BIP-I showed associations in the opposite direction. Depression and BIP-II had negative correlations with relative amplitude. All mood disorders were positively correlated with daytime sleepiness. Overall, we observed both genetic commonalities and differences across mood disorders in their relationships with biological rhythms: depression and BIP-I differed the most, while BIP-II was in an intermediate position.The present results suggest that the clinically observed associations between mood disorders and biological rhythms have shared genetic underpinnings. Future research considering possible joint mechanisms may offer potential avenues for improving disease detection and treatment.