bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knowledge and Practice of Third Year Degree Nursing Students Regarding Postpartum Depression at a University: Windhoek, Namibia.
Authors: Kadhila Joseph Galukeni Joseph , Moongo Winnie Rauha 
Published: November 2022

Abstract
Postpartum depressions (PPD) are common, affecting at least 10% of mothers. PPD is a common illness, the global prevalence being 10–15%. The prevention, detection and treatment of postpartum depression (PPD) are of great importance because PPD without care may cause many problems. Postpartum depressions are serious because of their impact on mother-baby relationships and on the Childs’ Development. However, these depressions seem very insufficiently diagnosed and treated. Methodology: In this study a quantitative descriptive research design was adopted. Descriptive research design is useful as it helps to obtain information that describes the existing phenomena by asking individuals about their perceptions, attitudes and values. The design reports things the way they are. In this regard, the descriptive research design was used to achieve the main objective of the study which is to assess the knowledge and practice of third year degree nursing students at a University, Windhoek Namibia, regarding postpartum depression. Result: Participant have good knowledge on, knowledge and practice regarding postpartum depression. An analysis of student’s knowledge about whether physical stress can predispose a woman to PPD revealed that clearly more than half of participants (89.6%, n = 60) agreed, while only 9.0% (n = 6) of the participants chosen neutral and 1.5%(n = 1) disagreed that physical stress can predisposes a woman to PPD. Conclusion: Descriptive analysis was conducted to compare the knowledge of study participants on postpartum depression. In addition, it shows that only few students who’s disagreed that smoking can predispose woman to PPD. The study revealed that the majority of the participant agreed that physical stress can predispose a woman to PPD. Overall, the study results revealed that above average of the 3rd year nursing students has good knowledge on postpartum depression.
The concept of detection of dynamic lung hyperinflation using cardiopulmonary exercise testing
Authors: Kominami Kazuyuki 
Published: November 2022

Abstract
Background
Dynamic lung hyperinflation (DLH) caused by air trapping, which increases residual air volume, is a common cause of shortness of breath on exertion in chronic obstructive pulmonary disease (COPD). DLH is commonly evaluated by measuring the decrease in maximal inspiratory volume during exercise, or using the hyperventilation method. However, only a few facilities perform these methods, and testing opportunities are limited. Therefore, we investigated the possibility of visually and qualitatively detecting DLH using data from a cardiopulmonary exercise test (CPET).
Methods
Four men who underwent symptom-limiting CPET were included in this study, including a male patient in his 60s with confirmed COPD, a 50s male long-term smoker, and two healthy males in their 20s and 70s, respectively. We calculated the difference between the inspiratory tidal volume (TV I) and expiratory tidal volume (TV E) per breath (TV E-I) from the breath-by-breath data of each CPET and plotted it against the time axis.
Results
No decrease in TV E-I was observed in either of the healthy males. However, in the patient with COPD and long-term smoker, TV E-I began to decrease soon after the start of exercise.
Conclusion
These results indicate that DLH can be visually detected using CPET data. However, this study was a validation of a very limited number of cases, and a comparison with existing evaluation methods and verification of disease specificity are required.
Differentiation of Enteric Neural Crest Cells Transplanted From Sox10-venus Mouse Embryonic Stem Cells Into the Gut of the Endothelin Receptor B Null Mouse Model
Authors: Fujiwara Naho , Miyahara Katsumi , Nakazawa-Tanaka Nana , Oishi Yoshie , Akazawa Chihiro , Tada Norihiro , Yamataka Atsuyuki 
Published: November 2022

Abstract
Purpose
Failure of enteric neural crest-derived cells (ENCCs) to correctly colonize the embryonic gut results in Hirschsprung’s disease (HD). Embryonic stem cells (ESCs) have the potential to differentiate into all tissue-specific cells and lineages, including ENCCs. We investigated the cellular differentiation of ESCs from Sox10-Venus + mice into both control and endothelin receptor-B knockout (Ednrb KO) mouse gut to assess each region.
Methods
We established ESCs from Sox10-Venus + mice. These cells were cultured for two days, then selected and co-cultured with either a dissociated control or Sox10-Venus – Ednrb KO mouse gut (both small intestine and colon) on embryonic day (E) 13.5. Four days later, cells were immunolabeled for Tuj1 and visualized using confocal microscopy.
Results
Confocal microscopy revealed that transplanted Sox10-Venus + cells from ESCs migrated extensively within the host gut. Moreover, Tuj1-positive neurites were detected in the transplanted ESCs. Tuj1 expression was significantly decreased in aganglionic HD colon compared to controls (p<0.05) and the HD small intestine (p<0.05).
Conclusions
This study demonstrated that an appropriate host environment is crucial for normal and complete colonization of the gut. Further investigations are required to confirm whether modifying this environment can improve the results of this model.
Metformin Regulates Autophagy to Attenuate Mitochondrial Dysfunction in High Glucose-stimulated H9C2 Cardiomyocytes via the AMPKα/Sirt1/Parkin Signaling Axis
Authors: Zhang Zhiliang , Ling Yesheng , Huang Zhuoshan , Zhong Junlin , Chen Qian , Liu Jinlai , Tang Xixiang , Peng Long 
Published: November 2022

Abstract
Purpose: Mitophagy reduces mitochondrial dysfunction and
accumulation of reactive oxygen species (ROS) to prevent myocardial
injury in diabetic cardiomyopathy (DCM). Accumulated studies have
confirmed that metformin enhances autophagy to maintain
mitochondrial homeostasis and scavenge ROS. However, whether and
how metformin regulates mitophagy in cardiomyocytes remain
unclear.

Methods: Diabetic cardiomyopathy was modeled in H9c2
Cardiomyocytes treated with high glucose (30 mM) . Then high
Glucose-stimulated H9C2 cells were exposed to metformin, AMPKα
inhibitor and Sirt1 inhibitor for 24 h. Mitochondrial dysfunction
and mitophagy were detected by fluorescent probe
2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA), mitochondrial
membrane potential(MMP), flow cytometry and western blot.

Resluts: We found that the protein expression of Parkin, ROS
level, and mitochondrial membrane potential showed dynamic changes
in H9C2 cells under the stimulation of high glucose. Importantly,
metformin enhanced mitophagy, scavenged ROS, improved mitochondrial
function and inhibited apoptosis in H9C2 cells treated with high
glucose. Mechanistically, metformin increased the protein
expression of p-AMPKα, Sirt1, Parkin, and LC3-II in H9C2 cells
after a high glucose challenge. Depletion of AMPKα and Sirt1
abolished the increase of protein levels of Parkin and LC3-II and
mitophagy levels induced by metformin.

Conclusion: Our data indicated that metformin improves
mitochondrial dysfunction of H9C2 cells under hyperglycemia by
activating AMPKα/Sirt1/Parkin-mediated mitophagy, which provides
novel evidence for the treatment of DCM.
Osteoid Osteoma of the Quadrilateral plate of acetabulum: A case report on an unusual cause of hip pain
Authors: Virk Jagandeep Singh , Sanga Sunil , Chawla Manju , Rana Shaleen , Bhaker Poonam 
Published: November 2022

Abstract
Introduction– Osteoid Osteomas are rare and extremely painful benign bone tumours. They are usually seen in children in the growing age group and young adults. The patients typically complain of bony pain during the night time and usually relieved with rest and salicylates. Osteoid Osteomas are commonly encountered in the limbs with Proximal femur/ Hip region and Tibial diaphysis being the usual locations. Pelvic locations are rare for osteoid osteoma and few cases have been published/ reported in literature.
Case Presentation – A young female with long standing hip pain was diagnosed to have osteoid osteoma in an extremely rare location in her pelvic bone. After careful evaluation of the imaging the lesion was found to be in the Quadrilateral plate/Medial wall of acetabulum. After through pre-surgical planning she underwent the Gold-standard treatment for osteoid osteoma i.e., Computed Tomography (CT)- guided Radiofrequency Ablation (RFA). Immediate post-procedure the patient reported complete relief of her symptoms and has been symptoms-free for the past 2years of follow-up.
Conclusion - A high level of clinical suspicion is warranted to keep osteoid osteoma as a differential diagnosis in a patient presenting with the relevant symptoms. They can be easily missed on Radiographs and CT is a clincher for diagnosis and unusual anatomic sites can harbour the same. A carefully planned Radiofrequency ablation under CT-guidance gives favourable and reliable results for treatment of osteoid osteomas.
Tumor cell density dependent IL-8 secretion induces the fluctuation of Tregs/CD8+ T cells infiltration in hepatocellular carcinoma: one prompt for the existence of density checkpoint
Authors: Yan Mengchao , Yao Jia , Lin Yan , Yan Jun , Xie Ye , Zhou Yongqiang , Wei Jiayun , Li Xun 
Published: November 2022

Abstract
Background
Tumor cell density is a basic pathological feature of solid tumors. Chemotherapy, radiotherapy and targeted therapy reduce tumor cell density, while unrestricted tumor cell proliferation promotes this feature. The impact of tumor cells on the microenvironment during the process of tumor cell density from low to high is still unclear. In this study, we focused on the response mode of key immune cell subsets to tumor cell density in hepatocellular carcinoma (HCC).
Methods
We determined the density of tumor cells and immune cells in the same area by section staining. We identified mediator through PCR, Elisa, 3D culture, co culture, flow cytometry and lentivirus intervention. The mechanism of lactate promotion was verified by lactate test kit, bioinformatic methods, western blot and the above methods. The IL-8/DAPK1/lactate/Tregs axis was verified by the mouse liver cancer model. Tumor mutation burden was calculated from maftools in R.
Results
We found that tumor cell density induced fluctuation in Tregs/CD8+ T cells ratio by secreting IL-8, and IL-8 promoted Tregs infiltration through DAPK1/PK activity/lactate axis. Based on tumor ploidy and mutation burden data, we discussed the potential significance of immune fluctuation in the homeostasis of HCC mutation burden and proposed “density checkpoint” and “entropy model” to describe this phenomenon.
Conclusion
In summary, we report the infiltration mode of Tregs/CD8+ T cells in response to tumor cell density, and the mechanism and significance of this infiltrating mode in HCC. In addition, we provide new theoretical basis for IL-8 as a therapeutic target and the selection of immunotherapy window period in HCC.
Itaconate Inhibits Corticosterone-Induced Necroptosis and Neuroinflammation via Up-regulating Menin in HT22 Cells
Authors: Liang Jin-Yu , Gao Shan , Jiang Jia-Mei , Zhang Pin , Zou Wei , Tang Xiao-Qing , Tang Yi-Yun 
Published: November 2022

Abstract

Corticosterone (CORT) damages hippocampus neurons as well as induces neuroinflammation. Tricarboxylic acid cycle metabolite itaconate has an anti-inflammatory role. Necroptosis acts as programmed cell death triggering neuroinflammation. The deficiency of Menin, a multifunctional scaffold protein, aggravates neuroinflammation. In this study, we explored whether itaconate inhibits CORT-induced neuroinflammation and necroptosis as well as the mediatory role of Menin in this protective effect of itaconate using an exposure of CORT to HT22 hippocampal neuronal cells. The viability of HT22 cells was examined by the Cell Counting Kit 8 (CCK-8). The morphology of HT22 cells was observed by transmission electron microscope (TEM). The expressions of necroptosis-related proteins (p-RIP1/ RIP1, p-RIP3/ RIP3, and p-MLKL/ MLKL) were evaluated by Western blotting. The contents of inflammatory factors were detected by an enzyme-linked immunosorbent assay kit. Our results showed that CORT increases the contents of pro-inflammatory factors (IL-1β, TNF-α) as well as decreases the contents of anti-inflammatory factors (IL4, IL10) in HT22 cells. We also found that CORT increases the expressions of necroptosis-related proteins (p-RIP1/ RIP1, p-RIP3/ RIP3, and p-MLKL/ MLKL) and decreases the cell viability in HT22 cells, indicating that CORT induces necroptosis to HT22 cells. Itaconate improves CORT-induced neuroinflammation and necroptosis. Furthermore, itaconate upregulates the expression of Menin in CORT-exposed HT22 cells. Importantly, silencing Menin abolishes the antagonistic effect of itaconate on CORT-induced necroptosis and neuroinflammation. In brief, these results indicated that itaconate protects HT22 cells against CORT-induced neuroinflammation and necroptosis via upregulating Menin.
Protective effect of urotensin Ⅱ receptor antagonist Urantide and exercise training on doxorubicin-induced cardiotoxicity
Authors: Sun Jing , Zhou Jiedong , Sun Shimin , Lin Hui , Zhang Hanlin , Zhong Zuoquan , Guo HangYuan , Chi Jufang 
Published: November 2022

Abstract
Doxorubicin (DOX) has a wide antitumor spectrum, but its adverse cardiotoxicity may lead to heart failure. Urotensin II (UⅡ) is the most potent vasoconstrictor in mammals. It plays a role by activating the UII receptor (UT), the orphan G protein-coupled receptor (GPR14), collectively referred to as the UII/UT system. In the new version of "Chinese expert consensus on cardiac rehabilitation of chronic heart failure," it is pointed out that exercise rehabilitation is the cornerstone of cardiac rehabilitation. In this study, in vitro and in vivo assessments were performed using DOX-treated H9C2 cells and rats. It was found that the UT antagonist Urantide and exercise training improved DOX-induced cardiac insufficiency, reduced DOX-induced cardiomyocyte apoptosis, improved the structural disorder of myocardial fibers, and inhibited DOX-induced myocardial fibrosis. Further studies showed that Urantide alleviated DOX-induced cardiotoxicity by downregulating the expression levels of the p38 mitogen-activated protein kinase signaling pathway.
Nomogram-based Survival and Prognosis Prediction for HCC after surgery: analysis of the multi-center patients
Authors: Luo Biyuan , Liu Xianling 
Published: November 2022

Abstract
Objective: Hepatocellular carcinoma(HCC),the most prevalent form of liver cancer, owns high morbidity and mortality. The radical surgery is the preference. It is of great clinical significance to predict the postoperative survival.
Methods: All clinical characteristics of 1187 patients participants from multicenter were collected. We identified several indicators significantly associated with HCC survival through logistic analysis to develop the prediction model. Further analysis revealed the independent predictive capacity of the predictive model. A nomogram comprising the predictive model was established. The decision curve analysis(DCA), receiver operating characteristic (ROC) curve analysis and Kaplan-Meier analysis confirmed the good performance of the predictive model.
Results: As a result, we identified several clinical indicators that were significantly associated with HCC survival through univariate analysis and multivariate analysis. The predictive model was consist of clinical features and tumor characteristics readily obtained after surgery. All the factors above were incorporated into the nomogram and the application of the nomogram gave good discrimination and good calibration. Calibration curves showed a favorable consistency between the predicted probabilities. ROC curve analysis showed that the nomogram had good discrimination both in the training group and validation group, respectively. Moreover, decision curve analysis has been implemented to evaluate and compare prediction nomogram. Kaplan-Meier analysis showed significant differences in prognosis among different risk groups.
Conclusion: The study provides a novel model for predicting HCC patients undergone radical surgery.
Current state and enhancement pathways of biocultural diversity: a systematic review
Authors: Zhao Ye , Zhao Yijun , Wu Chao , Yu Zhaowu 
Published: November 2022

Abstract
A considerable number of field cases and review studies have contributed to the remedial conservation and promotion of the world's biocultural diversity, but few reviews have attempted to systematically and retrospectively analyze the existing studies. Therefore, this study aims to explore the paradigm model and research focus of current BCD studies, to expand the biocultural diversity enhancement pathways, and to reveal the gaps and guide future directions. We used grounded theory as a tool to code 111 high-quality literature abstracts (2010–2022) screened in related fields. We found: (1) the causal conditions for BCD research in the paradigm model are primarily the negative impact of human activities, while the actions and consequences are primarily focused on specific resource conservation, conceptual and approach exploration; (2) the integration of the relevant logic chains yields two major research focuses: biocultural heritage and biocultural approaches; (3) three areas of BCD enhancement pathways are identified: Resources Protection, Academic Integration, and Governance Strategy. We suggest that the BCD research lacks the relevant evaluation system and its application in an urban context, and future efforts should be made in terms of official intervention, concept expansion, and decision-making. This review may provide the theoretical basis for the practice and promotion of BCD in a global context.
Evaluation of Sugar, HMF, pH, and Acidity Content in Some Popular Soft Drinks Sold in Turkey
Authors: Gün Ramazan , Dursun Inan 
Published: November 2022

Abstract
Consumption of soft drinks has been increasing last decades especially amongst children worldwide due to the taste and their relatively cheap prices. It is well-known that high consumption of sugary drinks cause negative effects on human health. It is due to the fact some governments took actions as introducing ‘sugar tax’ on beverages recommended by the World Health Organisation (WHO) aimed to reduce consumption of sugary drinks. This study aims to determine sugar, HMF, pH, and titratable acidity values of soft drinks (n = 25) including fruit (n = 10) and vegetable (n = 1) juices, sodas (n = 4), iced teas (n = 3), energy drinks (n = 3), cokes (n = 2), iced coffee (n = 1), and malt drink (n = 1). All the samples were purchased from chain supermarkets in city of Bingöl, Turkey. HPLC-RID and HPLC-DAD were used to determine sugar profile (glucose, fructose, sucrose, and maltose) and HMF content of the samples respectively. Results showed that total sugar and HMF contents of the samples were found to be ranged from 0.23 to 13.85% and from 0.65 to 27.76 mg/L respectively. In 9 out of 25 samples HMF were not detected. Furthermore, pH and acidity values of the samples were found to be varied from 1.96 to 6.34 and from 0.73 to 22.07 mEq/L, respectively. The samples were then classified based on their total sugar content (from sugar free to very high sugar) and pH erosive capacity (from minimally erosive to extremely erosive).
Colorectal cancer-derived small extracellular vesicles induce TGFβ1-mediated epithelial to mesenchymal transition of hepatocytes
Authors: Pucci Marzia , Moschetti Marta , Urzì Ornella , Loria Marco , Conigliaro Alice , Bella Maria Antonietta Di , Crescitelli Rossella , Bagge Roger Olofsson , Santos Mark F , Puglisi Caterina , Forte Stefano , Lorico Aurelio , Alessandro Riccardo , Fontana Simona 
Published: November 2022

Abstract
Background: The liver is the main metastatic site for patients with colorectal cancer (CRC) and represents the most frequent cause of death for patients affected by this cancer. In recent years, a number of studies have highlighted the crucial role played by small extracellular vesicles (sEVs) released by cancer cells in initiating pre-metastatic niche formation in the liver, specifically affecting the activities of non-parenchymal cells as Kupffer cells and hepatic stellate cells, while the role of the hepatocytes still remains unknown. Even if this cell component is the most conspicuous in the liver and it is responsible for several physiological activities of this complex organ, its role in liver metastasis is only partly described and related to tumour cell colonization, while no data is available about its enrolment during the pre-metastatic niche formation.
Methods: sEVs were isolated from SW480 and SW620 CRC cells through differential centrifugation, quantified using the Nanosight technology and characterized by transmission electron microscope and western blot. The presence of TGFβ1 on the surface of CRC_sEVs was assessed by western blot, super-resolution fluorescence microscope, and ELISA. Functional and epithelial to mesenchymal transition (EMT) markers were analyzed through RT PCR, ELISA assay, western blot, and confocal analysis in human healthy hepatocytes (THLE-2 cells) treated with sEVs derived from CRC cells (CRC_sEVs) at different time points.
Results: Our study shows for the first time that CRC_sEVs carrying the transforming growth factor‑β1 (TGFβ1) impair the morphological and functional properties of human healthy hepatocytes by triggering their TGFβ1/SMAD-dependent EMT. These abilities of CRC_sEVs were further confirmed by evaluating the effects elicited on hepatocytes by sEVs isolated from plasma and biopsies from CRC patients.
Conclusions: Since it is known that EMT of hepatocytes leads to the formation of a fibrotic environment, a well-known driver of metastasis, these results suggest that CRC_sEV conditioned hepatocytes could have an active and until now neglected role during the liver metastasis formation.
Modified micro-marsupialization as an alternative treatment for ranulas
Authors: Yildirim Ayhan , Hertach René , Yildirim Ali , Yildirim Vedat 
Published: November 2022

AbstractThe anatomical location of ranulas poses an enormous treatment challenge for the dentist. Due to a number of elementary structures, the regional complexity of the area requires a very high level of expertise on the part of the practitioner. The correction of surgical interventions in this area requires great effort. Although complete excision with removal of the sublingual gland remains the gold standard of treatment, recent literature highlights successful results through simple modification of the conventional technique of granular marsupialization. This micromarsupialization option is minimally invasive and beneficial due to the shorter procedure time, minimal discomfort, and lack of postoperative complications. The following case report highlights the successful management of a case using a modified micro-marsupialization technique in an adult.