bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro and Pharmacoinformatics-based phytochemical screening for anticancer impacts of pistachio hull essential oil on AGS, PLC/PRF/5, and CACO2 cell lines
Authors: Askari Nahid , Parvizpour Sepideh , Marashi Seyed Mohammad Bagher , Baghery Fatemeh , Falahati-pour Soudeh Khanamani 
Published: July 2022

Abstract
The essential oil of pistacia vera (cv. Ohadi) hull (PHEO) was checked using gas chromatography mass spectrometry (GC/MS) analysis. It was studied the genes of the wnt pathway with a certain concentration of PHEO on Human gastric cancer (AGS), human hepatocellular carcinoma (PLC/PRF/5), and human colon cancer (CACO2) cell lines. After evaluating the survival rate of cancer cells by MTT test and determining IC50, pistachio hull essential oil (PHEO) was used for 24-hours to treat the cells. After RNA extraction, the expression of wnt pathway genes was evaluated by Real-Time PCR. Considering the crucial role of beta-catenin accumulation and its effect on the progression of gastrointestinal cancers, Western blot analysis was also used to determine the effect of PHEO in protein expression of β-catenin inhibition. Also, an in silico analysis was carried out to investigate the effect of PHEO extracted compounds on protein expression of β-catenin and FZD7 inhibition. According to the results, wnt pathway genes were changed in samples treated using PHEO. The results showed the up-regulation of GSK-3β and down-regulation of Wnt-1, LEF-1, TCF1, and CTNNB1 genes compared to the control. We showed inhibition of β-catenin protein in cancer cell lines. Four compounds of PHEO were suggested to have an inhibition effect on β-catenin and FZD7. These compounds can be useful in the treatment of gastrointestinal cancers. Altogether, the inhibitory role of β-catenin protein can be very effective and can be considered one of the therapeutic goals in the treatment of gastrointestinal cancers.
Comparison of six-minute walk test distance vs. estimated maximum oxygen consumption for predicting postoperative pulmonary complications in patients undergoing upper abdominal surgery: a prospective cohort study
Authors: Yin Xin , Wang Jingwen , Xu Zhibo , Qian Fuyong , Liu Songbin , Cai Yuxi , Jiang Zhaoshun , Zhang Xixue , Gu Weidong 
Published: July 2022

Abstract
Objective
Estimated maximum oxygen consumption (eVO2max) is superior to six-minute walk distance (6MWD) in predicting functional capacity. The hypothesis that eVO2max is a better predictor of postoperative pulmonary complications (PPCs) than 6MWD in patients undergoing major upper abdominal surgery is evaluated.
Method
Patients scheduled for elective major upper abdominal surgery from July 2018 to May 2021 were included. The six-minute walk test was carried out on all patients before surgery. eVO2max was calculated using the Burr equation incorporated with 6MWD, age, gender, weight, and resting heart rate. The patients were divided into the PPC and the non-PPC group according to the occurrence of clinically significant PPCs. The sensitivity, specificity, and optimum cutoff point of 6MWD and eVO2 max were analyzed in the prediction of PPCs. The area under the receiver operating characteristic curve (AUC) of 6MWD vs. eVO2max was compared by Z test.
Results
A total of 308 patients were included in this study; 71/308 developed clinically significant PPCs. Compared to the non-PPCs group, the value of 6MWD (P < 0.001) and eVO2max (P < 0.001) were significantly lower in the PPC group. The optimum cutoff point for 6MWD in predicting PPCs was 372. 5m with a sensitivity of 63.4% and specificity of 79.3%. The optimum cutoff point for eVO2max was 8.8 MET with a sensitivity of 91.6% and specificity of 79.3%. A significantly increased AUC was observed in eVO2max compared to 6MWD (P < 0.001, Z = 4.713).
Conclusion
eVO2max has a better validity than 6MWD for predicting PPCs in patients undergoing major upper abdominal surgery. The results suggested that eVO2max has the potential to be a valid tool to screen patients with a high risk of PPCs.
Lymphopenia and local antigen presentation combine to induce colitis in a transgenic mouse model of uveitis
Authors: Mölzer Christine , Liu Yi-Hsia , Muckersie Elizabeth , Klaska Izabela P. , Cornall Richard , Wilson Heather M. , Kuffová Lucia , Forrester John V. 
Published: July 2022

Abstract
Undifferentiated uveitis (intraocular inflammation, IOI) is an idiopathic sight-threatening, presumed autoimmune disease, accountable for ~ 10% of all blindness in the developed world. We have investigated the association of uveitis with inflammatory bowel disease (IBD) using a mouse model of spontaneous experimental autoimmune uveoretinitis (EAU). Mice expressing the transgene (Tg) hen egg lysozyme (HEL) in the retina were crossed with 3A9 mice expressing a transgenic HEL-specific TCR. Double transgenic (dTg TCR/HEL) mice with EAU also spontaneously develop clinical signs of colitis at ~ P30 (post-partum day) with diarrhoea, bowel shortening, oedema and lamina propria (LP) inflammatory cell infiltration. Single (s)Tg TCR (3A9) mice also show histological LP cell infiltration but no clinical signs. dTg TCR/HEL mice are profoundly lymphopenic at weaning. In addition, dTg TCR/HEL mice contain myeloid cells which express MHC Class II-HEL peptide (MHCII-HEL) complexes, not only in the inflamed retina but also in the colon. In this model the lymphopenia and reduction in the absolute Treg numbers in dTg TCR/HEL mice is sufficient to initiate eye disease. We suggest that cell-associated antigen released from the inflamed eye, activates colonic HEL-specific T cells which, in a microbial micro-environment, not only cause colitis but feed-back to amplify IOI.
DNA sequencing sheds light on the evolutionary history of peanut and identifies genes associated with phenotypic diversification
Authors: Zheng Zheng , Sun Ziqi , Qi Feiyan , Fang Yuanjin , Lin Ke , Pavan Stefano , Huang Bingyan , Dong Wenzhao , Du Pei , Tian Mengdi , Shi Lei , Xu Jing , Han Suoyi , Liu Hua , Qin Li , Zhang Zhongxin , Dai Xiaodong , Miao Lijuan , Zhao Ruifang , Wang Juan , Bai Yuling , Visser Richard , Zhang Xinyou 
Published: July 2022

Abstract

Cultivated peanut (Arachis hypogaea L.) is one of the most widely grown oilseed crops worldwide, however the events leading to its origin and diversification are not fully understood. Here, by combining chloroplast and whole genome sequence data from a large germplasm collection, we show that the two A. hypogaea subspecies (hypogaea and fastigiata) likely arose from distinct allopolyploidization and domestication events. Peanut genetic clusters were then differentiated in relation to dissemination routes and breeding efforts. A combination of linkage mapping and genome-wide association studies allowed us to characterize genes and genomic regions related to main peanut morpho-agronomic traits, namely inflorescence architecture, inner integument color, growth habit, pod/seed weight, and oil content. Together, our findings shed light on peanut evolutionary history and provide an important genomic framework resource for the genetic improvement of this crop.
Incidence of organ involvement in Behcet’s disease: a historical cohort study
Authors: Banihashemi Seyed Arash Tehrani , Solaymani-Dodaran Masoud , Faezi Seyedeh Tahereh , Shahram Farhad , Paragomi Pedram , Moradi Kamran , Mohammadi Faezeh , Nejadhosseinian Mohammad , Davatchi Fereydoun 
Published: July 2022

Abstract
Objective: The objective of this study was to assess the incidence of BD-related serious organ involvements in a sizeable cohort of patients with Behcet’s disease.Methods: In a retrospective cohort study 1166 patients with Behcet’s disease whose first visit to BD clinic was registered between April 1995 and March 2001 were enrolled and followed up until March 2012 for ocular, vascular, joint, Central Nervous System (CNS), and epididymis involvement. Incidence density and cumulative incidence of different organ lesions from the disease onset were estimated.Results: Age at disease onset was 25.1 ±9.46 (mean ± SD) years and the male to female ratio was 1.34:1. The incidence density (per 100 person-year) of ocular involvement was 7.6 (CI 95%: 7.08 – 8.25), joint involvement was 1.32 (CI 95%: 1.13- 1.54), vascular involvement was 0.66 (CI 95%: 0.53-0.81), epididymitis/orchitis was 0.67 (CI 95%: 0.5-0.88) and CNS involvement was 0.14 (CI 95%:0.09-0.22). The incidence density of blindness was 1.63 per 100 person-year (CI 95%: 1.42-1.87). The cumulative incidence of different organ involvement 10 years after the onset of Behcet’s disease was 53.2% for ocular lesions, 6.4% for vascular lesions, 12.4% for joint lesions, and 6.4% for Epididymitis/Orchitis (in male patients).Conclusions: Ocular involvement occurred in almost half of the patients within the 10 years after disease onset. At a lower rate but not less important, other inflammatory organ involvement like vasculitis, arthritis, epididymitis and CNS involvement may occur in the BD patients.
Genetic mapping of quantitative trait loci associated with drought tolerance in chickpea (Cicer arietinum L.)
Authors: Yadava Yashwant K. , Chaudhary Pooja , Yadav Sheel , Rizvi Aqeel Hasan , Kumar Tapan , Shrivastava Rachna , Soren KR , Bharadwaj C , Sriniva R , Singh NK , Jain PK 
Published: July 2022

Abstract
Elucidation of the genetic basis of drought tolerance is vital for genomics-assisted breeding of drought tolerant crop varieties. Here, we used genotyping-by-sequencing (GBS) to identify single nucleotide polymorphisms (SNPs) in recombinant inbred lines (RILs) derived from a cross between a drought tolerant chickpea variety, Pusa 362 and a drought sensitive variety, SBD 377. The GBS identified a total of 35,502 SNPs and subsequent filtering of these resulted in 3,237 high-quality SNPs included in the eight linkage groups. Fifty-one percent of these SNPs were located in the genic regions distributed throughout the genome. The high density linkage map has total map length of 1069 cM with an average marker interval of 0.33 cM. The linkage map was used to identify 15 robust QTLs for four drought related traits viz. membrane stability index, relative water content, seed weight and yield under drought, with percent variance explained within the range of 11.5–26.7% and LOD scores of 5.58 to 6.89, which were located on six of the eight linkage groups. A genomic region on LG 6 harbors quantitative traits loci (QTLs) explaining > 25% phenotypic variance for membrane stability index, yield and > 10% PVE for 100 seed weight. This study also provides the first report of major QTLs for physiological traits such as membrane stability index and relative water content for drought stress in chickpea. A total of 969 putative candidate genes were identified in the 15.6 Mb genomic region spanning these QTLs. In-silico expression profiling based on the available transcriptome data revealed that 617 of these genes were differentially expressed under drought stress. The novel genomic regions and candidate genes identified will be useful in genomics-assisted breeding of superior drought tolerant chickpea cultivars.
B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation
Authors: Stoler-Barak Liat , Harris Ethan , Peres Ayelet , Hezroni Hadas , Kuka Mirela , Grenov Amalie , Gurwicz Neta , Kupervaser Meital , Yip Bon Ham , Iannacone Matteo , Yaari Gur , Crispino John , Shulman Ziv 
Published: July 2022

Abstract
Protection from viral infections depends on immunoglobulin isotype switching, which endows antibodies with effector functions. Here, we found that the protein kinase DYRK1A is essential for B cell-mediated protection from viral infection and effective vaccination through regulation of class switch recombination (CSR). Dyrk1a-deficient B cells were impaired in CSR activity in vivo and in vitro. Phosphoproteomic screens and kinase-activity assays identified MSH6, a DNA mismatch repair protein, as a direct substrate for DYRK1A, and deletion of a single phosphorylation site impaired CSR. After CSR and germinal center seeding, DYRK1A was required for proper clonal expansion of antigen-specific B cells through attenuation of proliferation. These findings reveal DYRK1A-mediated biological mechanisms of B cell immune responses that may be used for manipulation in antibody-mediated autoimmunity.
Dietary Epimedium extract supplementation improves intestinal functions and alters gut microbiota in broilers
Authors: Zhang Jiaqi , Yu Haitao , Zhang Huiyan , Zhao Qingyu , Si Wei , Qin Yuchang , Zhang Junmin 
Published: July 2022

Abstract
Background
Growth-promoting antibiotics have been banned by law in the livestock and poultry breeding industry in many countries. Various alternatives to antibiotics have been investigated for use in livestock. Epimedium (EM) is an herb rich in flavonoids that has many beneficial effects on animals. Therefore, this study was planned to explore the potential of EM as a new alternative antibiotic product in animal feed.
Methods
A total of 720 1-day-old male broilers (Arbor Acres Plus) were randomly divided into six groups and fed basal diet (normal control; NC), basal diet supplemented with antibiotic (75 mg/kg chlortetracycline; CTC), and basal diet supplemented with 100, 200, 400 or 800 mg/kg EM extract for 6 weeks (EM100, EM200, EM400 and EM800 groups). The growth performance at weeks 3 and 6 was measured. Serum, intestinal tissue and feces were collected to assay for antioxidant indexes, intestinal permeability, lactic acid and short-chain fatty acids (SCFAs) profiles, microbial composition, and expression of intestinal barrier genes.
Results
The average daily feed intake in CTC group at 1–21 days was significantly higher than that in the NC group, and had no statistical difference with EM groups. Compared with NC group, average daily gain in CTC and EM200 groups increased significantly at 1–21 and 1–42 days. Compared with NC group, EM200 and EM400 groups had significantly decreased levels of lipopolysaccharide and D-lactic acid in serum throughout the study. The concentrations of lactic acid, acetic acid, propionic acid, butyric acid and SCFAs in feces of birds fed 200 mg/kg EM diet were significantly higher than those fed chlortetracycline. The dietary supplementation of chlortetracycline and 200 mg/kg EM significantly increased ileal expression of SOD1, Claudin-1 and ZO-1 genes. Dietary supplemented with 200 mg/kg EM increased the relative abundances of g_NK4A214_group and Lactobacillus in the jejunum, while the relative abundances of Microbacterium, Kitasatospora, Bacteroides in the jejunum and Gallibacterium in the ileum decreased.
Conclusions
Supplementation with 200 mg/kg EM extract improved the composition of intestinal flora by regulating the core bacterial genus Lactobacillus, and increased the concentration of beneficial metabolites lactic acid and SCFAs in the flora, thereby improving the antioxidant capacity and intestinal permeability, enhancing the function of tight junction proteins. These beneficial effects improved the growth performance of broilers.
Bearing capacity of shallow rigid strip foundations near slopes based on generalized log-spiral shear mechanism
Authors: Chen Tingjun , Xiao Shiguo 
Published: July 2022

Abstract
In terms of five bilateral failure modes of shallow rigid strip foundations near slopes, kinematically admissible velocity fields are constructed using a generalized log-spiral shear mechanism improving the traditional failure mechanism by introducing a geometric factor. The upper-bound solution of the bearing capacity for the foundations near slopes is accordingly derived. The minimum of the five values is the required solution, and the corresponding failure mode is the accepted one. Some examples show the proposed results are fairly close to those by other theoretical and numerical methods or laboratory model tests, and in most cases the geometric parameter of the generalized mechanism is between 0.7 and 1, which means the failure mechanisms of the systems are considerably optimized. The influence of slope height, foundation depth and width, distance from the foundation to slope crest, and soil properties on the transformation among these failure modes is provided. Analysis results of many examples show that there is not rigorously linear relationship between the bearing capacity of foundations near slopes and cohesion or depth of the footing. Besides, a table of comprehensive factor expressed to reflect the slope effect on the bearing capacity for convenient applications.
Land suitability analysis for Mango using AHP method
Authors: Salunkhe Sanjani , Nandgude Sachin , Bhange Harshal 
Published: July 2022

Abstract
The present study determined the mango land suitability in Ratnagiri district with combine use of MCDM with GIS based AHP. There were five main criteria i.e. climate, topography, soil, erosion and conservation measures and eleven sub-criteria were used in the present study. The all main criteria maps were prepared in ArcGIS according to mango crop requirement. After preparing the various thematic maps, AHP process has been used to calculate the weights through pairwise comparison matrix. In order to accept the weighting results or judgments, a consistency ratio (CR) value was calculated to determine whether the pairwise comparison was consistent. Then the ‘Weighted Overlay’ tool in overlay toolset which is in spatial analyst tools in ArcGIS has been used to perform an overlay analysis. The results showed that 16.86 percent of the area was classified as highly suitable, while 67.93 percent, 10.03 percent, and 5.18 percent of the area were classified as moderately suitable, marginally suitable, and not suitable, respectively. As a result, a GIS-based AHP approach can provide better insights for improving capabilities and producing more realistic output scenarios.
Evaluation of factors related to maternal anxiety during pregnancy among women referred to Tabriz primary care centers
Authors: Mahini Elmira , Hakimi Sevil , Shahrokhi Hassan , Salahi Behnaz , Baniadam Khatereh Olad , Ranjbar Fatemeh 
Published: July 2022

Abstract
Background: Maternal anxiety during pregnancy is sometimes considered a normal mechanism to overcome the mother’s mental preoccupation with having a child. However, stress and anxiety might become a medical condition, becoming so severe as to affect the mother’s mental health. Therefore, the present study aimed to investigate factors related to maternal anxiety during pregnancy in women referring to prenatal primary care centers in Tabriz. Methods: The target population in the present cross-sectional study was the pregnant women referring to primary care centers affiliated with Tabriz University of Medical Sciences in 2018-2019 (n=533). Sampling was carried out using the random cluster technique (separately for municipal centers). The data were collected using the study tools, including a questionnaire on demographic data, prenatal anxiety screening scale (PASS), and researcher-made questions on maternal anxiety during pregnancy.Results: In the present study, 37.5% of pregnant women had anxiety. Of all the demographic and background variables, income (P=0.015), a history of preterm delivery (P=0.018), and unintended pregnancy (P=0.022) were significantly related to anxiety. Of the variables of income, a history of preterm delivery, and unintended pregnancy in the regression model, the odds of anxiety were 41% lower in pregnant women with somewhat adequate income than those with inadequate income after correcting for other variables (P=0.011). In addition, the variable of unintended pregnancy increased the odds of anxiety up to 49% after correcting for other variables (P=0.023).Conclusion: The present study showed that income and unintended pregnancy significantly affect maternal anxiety during pregnancy.Trial registration: The protocol of the study was approved by the Ethics Committee of Tabriz University of Medical Sciences under the code (IR.TBZMED.REC.1398.161).
The Impact of 4D-Flow MRI Spatial Resolution on Patient-Specific CFD Simulations of the Thoracic Aorta
Authors: Cherry Molly , Khatir Zinedine , Khan Amirul , Bissell Malenka 
Published: July 2022

Abstract
Magnetic Resonance Imaging (MRI) is considered the gold standard of medical imaging technologies as it allows for accurate imaging of blood vessels. 4-Dimensional Flow Magnetic Resonance Imaging (4D-Flow MRI) is built on conventional MRI, and provides flow data in the three vector directions and a time resolved magnitude data set. As such it can be used to retrospectively calculate haemodynamic parameters of interest, such as Wall Shear Stress (WSS). However, multiple studies have indicated that a significant limitation of the imaging technique is the spatiotemporal resolution that is currently available. Recent advances have proposed and successfully integrated 4D-Flow MRI imaging techniques with Computational Fluid Dynamics (CFD) to produce patient-specific simulations that have the potential to aid in treatments,surgical decision making, and risk stratification. However, the consequences of using insufficient 4D-Flow MRI spatial resolutions on any patient-specific CFD simulations is currently unclear, despite being a recognised limitation. The research presented in this study aims to quantify the error in patient-specific 4D-Flow MRI based CFD simulations that can be attributed to insufficient spatial resolutions when acquiring 4D-Flow MRI data. For this research, a patient has undergone four 4D-Flow MRI scans acquired at various isotropic spatial resolutions and patient-specific CFD simulations have subsequently been run using geometry and velocity data produced from each scan. It was found that compared to CFD simulations based on a 1.5mm x 1.5mm x 1.5mm, using a spatial resolution of 4mm x 4mm x 4mm substantially underestimated the maximum velocity magnitude at peak systole by 110.55%. The impacts of 4D-Flow MRI spatial resolution on WSS calculated from CFD simulations have been investigated and it has been shown that WSS is underestimated in CFD simulations that are based on a coarse 4D-Flow MRI spatial resolution. The authors have concluded that a minimum 4D-Flow MRI spatial resolution of 1.5mm x 1.5mm x 1.5mm must be used when acquiring 4D-Flow MRI data to perform patient-specific CFD simulations. A coarser spatial resolution will produce substantial errors within the flow field and geometry.
Effects of ZnO nanoparticles, plasticizer, and emulsifier on PLA films properties and mechanical aging stability
Authors: Tajari Neda , Sadrnia Hassan , Hosseini Fereshte 
Published: July 2022

Abstract
Polylactic acid is an environmentally friendly polymer that has the potential to be an alternative to petroleum-based materials in the food packaging industry. However, issues such as the fragility of the produced films, low thermal stability, etc. are obstacles to the widespread use of this type of film. In this work, films based on polylactic acid were produced with additives, namely zinc oxide nanoparticles (1 wt.% PLA), polyethylene glycol 400 (20 wt.% PLA), and tween 80 (0.25 wt.% solution). The purpose of adding additives was to improve the properties of the sample films. The results showed that the presence of plasticizer and emulsifier increases the hydrophilicity of the film and the water vapor permeability. The highest contact angle (85.33 [[EQUATION]] ±4.00) and the lowest water vapor permeability (0.074±0.002 g.mm/kPa.h.m 2 ) were related to neat PLA film. The effect of the added materials on the thickness of the films was not significant, and all the films had good transparency. The addition of nanoparticles, emulsifiers, and plasticizers increased the percentage of crystallinity. The highest crystallinity percentage was for film PLA/ZnO (7.7%). The effect of plasticizer and emulsifier on tensile strength and elastic modulus was more than nanoparticles. The effect of the type of film produced on the mechanical properties was significant, and the effect of their storage time was only significant on the elongation at break. These results can help to improve the physical, mechanical, and thermal properties of polylactic acid for food packaging on an industrial scale.