bluuebunny/crossref_metadata_2025_split huggingface dataset:
The genetic diversity of Asplenium viride (Aspleniaceae) fern colonizing heavy metal-polluted sites
Authors: Woch Marcin W Wiktor , Woch Marcin W. , Migdałek Grzegorz , Jedrzejczyk Iwona , Podsiedlik Marek , Stefanowicz Anna M. 
Published: March 2022

Abstract
Heavy metals can affect the morphology, physiology and evolution of plants. Asplenium viride is a diploid species, belonging to the largest genus of the cosmopolitan fern family Aspleniaceae, and occurring on various types of alkaline rocks. It is known to colonize sites with high concentrations of heavy metals, exhibiting changes in frond morphology. Microevolutionary processes, manifesting as changes in genome size and new genotype formation, can ultimately lead to the formation of new subspecies and speciation. This study aimed to evaluate the morphological and genetic diversity of A. viride, and to test for a potential correlation between variability and heavy metal concentration. Analysis of A. viride specimens, from one metalliferous site and five non-metalliferous localities, showed no apparent variation in genome size between plants from affected and non-affected sites. There was no significant correlation between genetic variability and heavy metal concentration. This was possibly due to intragametophytic selfing, caused by patchy habitats and subsequent founder effects, resulting from long-distance colonization by single spores.
Metabolomic biomarkers related to non-suicidal self-injury in patients with bipolar disorder
Authors: Ren Yan , Guo Xiangjie , Jia Jiao , Zhang Zhiyong , Miao Yuting , Wu Peng , Bai Yaqin 
Published: March 2022

Abstract
Background: Non-suicidal self-injury (NSSI) is an important symptom of bipolar disorder (BD) and other mental disorders and has attracted the attention of researchers lately. It is of great significance to study the characteristic markers of NSSI. Metabolomics is a relatively new field that can provide complementary insights into data obtained from genomic, transcriptomic, and proteomic analyses of psychiatric disorders. The aim of this study was to identify the metabolic pathways associated with BD with NSSI and assess important diagnostic and predictive indices of NSSI in BD.Method: Nuclear magnetic resonance spectrometry was performed to evaluate the serum metabolic profiles of patients with BD with NSSI (n = 31), patients with BD without NSSI (n = 46), and healthy controls (n = 10). Data were analyzed using an Orthogonal Partial Least Square Discriminant Analysis and a t-test. Differential metabolites were identified (VIP > 1 and p < 0.05), and further analyzed using Metabo Analyst 3.0 to identify associated metabolic pathways.Results: Eight metabolites in the serum and two important metabolic pathways, the urea and glutamate metabolism cycles, were found to distinguish patients with BD with NSSI from healthy controls. Eight metabolites in the serum, glycine and serine metabolism pathway, and the glucose-alanine cycle were found to distinguish patients with BD without NSSI from healthy controls. Five metabolites in the serum and the purine metabolism pathway were found to distinguish patients with BD with NSSI from those with BD without NSSI.Conclusions: Abnormalities in the urea cycle, glutamate metabolism, and purine metabolism played important roles in the pathogenesis of BD with NSSI.
Feasibility and safety of laparoscopic portal vein ligation prior to major hepatectomy for hepatocellular carcinoma
Authors: An Tailai , Guo Yusheng , Wang Yan , Bao Shiyun , Wu Tianchong , Liu Linsen , Wang Yawei , Bi Jiangang 
Published: March 2022

Abstract
Background: Patients with hepatocellular carcinoma (HCC) demonstrated to have an inadequate future liver remnant (FLR) on preoperative volumetric assessment are potential candidates for laparoscopic portal vein ligation (LPVL). Previous studies have reported that for patients with hepatic malignancies, laparoscopic portal vein ligation (LPVL) is efficient in increasing FLR, which, however, has not been solely reported in HCC. The purpose of this study was to evaluate the safety, feasibility and efficiency of LPVL prior to major hepatectomy for patients with HCC. Methods: Clinical information of HCC patients who had undergone laparoscopic portal vein ligation (LPVL) at our center was retrospectively reviewed and documented. Demographic, radiographic, clinical and volumetric details (both before and after LPVL) were retrieved to evaluate the feasibility and safety of LPVL prior to major hepatectomy for HCC. Results: Between April 2020 and December 2021, there were a total of 10 HCC patients undergoing LPVL as a preparation for subsequent major hepatectomy at our center. The mean age of these 10 patients was 61.30±8.83 years old. Of these 10 patients, 9 were male and only 1 was female. 9 patients underwent laparoscopic ligation of the right portal vein and one the left portal vein. All the patients, left or right portal veins were ligated by clips. After LPVL, the mean volume increased from 433.16±103.64 ml to 550.62±123.19 ml (P＜0.001）. All the 10 patients had adequate hypertrophy of FLR and subsequent major hepatectomy was performed as scheduled. No LPVL-associated complications were recorded. Conclusion: LPVL is both feasible and could be safely performed. For carefully selected patients, LPVL could be considered as a safe and feasible alternative to portal vein embolization (PVE) given the rather low complication rate and high efficiency of LPVL.
Cohesin erases genomic-proximity biases to drive stochastic Protocadherin expression
for proper neural wiring
Authors: Canzio Daniele , Kiefer Lea , Servito Gabrielle Isabelle , Rajkumar Sandy , Langen Jennifer , Chiosso Anna , Buckley Alexander , Cha Elizabeth , Horta Adan , Mui Michael 
Published: March 2022

Abstract
Clustered Protocadherin (Pcdh) proteins act as cell-surface recognition barcodes for neural circuit formation. Neurites expressing the same barcode repel each other, but this mechanism is deployed in two different ways. For instance, convergence of olfactory sensory neuron (OSN) projections requires stochastic expression of distinct Pcdh isoforms in individual cells, while tiling of neural arbors of serotonergic neurons (5-HTs) requires expression of the same isoform, Pcdhαc2. Despite their essential role, however, the molecular mechanisms of cell-type specific Pcdh barcoding remain a mystery. Here, we uncover a new role of cohesin: that of regulating distance-independent enhancer-promoter interactions to enable random Pcdh isoform choice via DNA loop extrusion in OSNs. Remarkably, this step mediates DNA demethylation of Pcdh promoters and their CTCF binding sites, thus directing CTCF to the chosen promoter. In contrast, the uniform pattern of Pcdh expression in 5-HTs is achieved through conventional cohesin-independent, distance-dependent enhancer/promoter interactions, that favor choice of the nearest isoform. Thus, cell-type specific cohesin deployment converts a distance-dependent and deterministic regulatory logic into a distance-independent and stochastic one. We propose that this mechanism provides an elegant strategy to achieve distinct patterns of Pcdh expression that generate wiring instructions to meet the connectivity requirements of different neural classes.
VALIS: Virtual Alignment of pathoLogy Image Series
Authors: Gatenbee Chandler , Baker Ann-Marie , Prabhakaran Sandhya , Slebos Robbert , Mandal Gunjan , Mulholland Eoghan , Leedham Simon , Conejo-Garcia Jose , Chung Christine , Robertson-Tessi Mark , Graham Trevor , Anderson Alexander 
Published: March 2022

Abstract
Interest in spatial omics is on the rise, but generation of highly multiplexed images used in many spatial analyses remains challenging, due to cost, expertise, methodical constraints, and/or access to technology. To remove these barriers, and thereby increase access to highly multiplexed images, we have developed the Virtual Alignment of pathoLogy Image Series (VALIS) software, which enables one to rapidly and easily generate highly multiplexed images by aligning (registering) any number of whole slide images (WSI) that were serially sliced and/or cyclically stained using immunohistochemistry (IHC) and/or immunofluorescence (IF). VALIS can save the registered WSI in the opensource ome.tiff format, facilitating downstream spatial analyses. Herein, we present two such examples, one based on a 32 marker IF image created by registering and merging 11 rounds of cyclic IF (CyCIF), and a second based on an 18 marker image created by registering, processing, and merging 18 brightfield images cyclically stained using IHC. In addition to being easy to use, VALIS is robust, having been tested with 273 IHC samples and 340 IF samples, each of which contained between 2-69 images per sample. The registered WSI tend to have low error and are completed within a matter of minutes. VALIS can also use the registration parameters to warp point data, such as cell centroids previously determined via cell segmentation. VALIS is written in Python and requires only few lines of code for execution. VALIS therefore provides a free, opensource, flexible, and easy to use pipeline for rigid and non-rigid registration of IF and/or IHC WSI, facilitating spatial analyses of prospective and existing datasets, breathing new life into the countless collections of brightfield and immunofluorescence images.
Analysis of Adverse Drug Events in Pulmonary Mycobacterium avium Complex Disease Using Spontaneous Reporting System
Authors: Namkoong Ho , Ozawa Takuya , Takaya Risako , Takahashi Yusuke , Fukunaga Koichi , Enoki Yuki , Taguchi Kazuaki , Kizu Junko , Matsumoto Kazuaki , Hasegawa Naoki 
Published: March 2022

Abstract
Background: In Japan, Mycobacterium avium complex lung disease (MAC-LD) is the most common in nontuberculous mycobacterial lung disease. Patients often experience adverse events, resulting in the discontinuation of treatment, which causes treatment failure. The Japanese Adverse Drug Event Report (JADER) database is a database of adverse events and reflects the clinical practice in Japan. We can collect large-scale data cost-effectively and detect signals of potential adverse events such as reporting odds ratio (ROR) by using spontaneous reporting systems. Methods: We included cases of MAC-LD between April 2004 and June 2017. We investigated sex, age, and medications that may have caused the adverse events, outcomes, and time of onset. We calculated the safety signal index as the ROR. Time-to-event analysis was performed using the Weibull distribution. Results: The total number of adverse effects of CAM, EB, and RFP was 2780, with 806 patients. In the overall adverse events, hematologic and lymphatic disorders were the most common adverse events, with 11%, followed by eye disorders (10.6%), and hepatobiliary disorders (8.9%). The outcomes were as follows: recovery, 40.1%; remission, 27.1%; non-recovery, 11.2%; and death, 7.2%. Regarding the most common onset time of CAM, EB, and RFP was within 120 days at 40%, 181–300 days at 43.6%, and within 120 days at 88.5%. For CAM, the RORs of infections and infestations, hepatobiliary system disorders, and immune system disorders were 4.13 (95% CI, 2.3–7.44), 2.61 (95% CI, 1.39–4.91), and 2.38 (95% CI, 1.04–5.44). For EB, the ROR of eye disorders was 215.79 (95% CI, 132.62–351.12). For RFP, the RORs of renal and urinary tract disorders and investigations were 7.03 (95% CI, 3.35–14.77) and 6.99 (95% CI, 3.22–15.18). The β value of EB was 2.07 (95% CI, 1.48–2.76), which was classified as wear-out failure type. Conclusions: For MAC-LD, adverse events of EB occur after 180 days, whereas the adverse events of CAM and RFP occur early in the course of treatment.
To Explore the Network Pharmacology and Molecular Docking Mechanism of Chaihu Shugan Powder with the “Same Treatment for Different Diseases” for Insomnia and Depression Based on the COVID-19 Pandemic
Authors: Wang Liang , Wang Peng , Chen Yingfan , Li Chen , Wang Xuelin , Li Mingwei , Luan Zhenxian , Zhang Yin , Li Shaodan , Yang Minghui 
Published: March 2022

Abstract
Background: The classic prescription Chaihu Shugan Powder (CHSGP) has been widely used in clinical Chinese medicine treatment and has clear clinical effects in the treatment of emotional diseases. Based on the increasing incidence of emotional diseases such as insomnia and depression in the population during the COVID-19 pandemic, we will explore the mechanism of CHSGP in the treatment of insomnia and depression with “Same Treatment for Different Diseases”. Methods: Using a bioinformatics and network pharmacology platform, protein database and STRING database, we collected CHSGP chemical composition and related target data and constructed a "component-target" action network through Gene Ontology and Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis. Molecular docking technology was used to verify key active ingredients and core targets. Results: A total of 119 active compounds of CHSGP were screened, such as quercetin, kaempferol, and β-sitosterol, and 113 common related targets overlapped with insomnia and depression. GO enrichment and KEGG pathway analysis mainly involved immune, inflammation, cell proliferation, apoptosis, endocrine and other related targets and signaling pathways. Molecular docking showed that small molecular compounds (kaempferol, luteolin, quercetin, 7-methoxy-2-methyl isoflavone and beta-sitosterol) had good binding effects with five target proteins (AKT1, IL1B, IL-6, FOS, GSK3B) to play a role in regulating immunity, the inflammatory response, cell proliferation, apoptosis, and endocrine signaling. Conclusions: Under the context of the COVID-19 pandemic, it revealed the complex mechanism of multicomponent, multitarget, and multipathway of the classic CHSGP for insomnia and depression, laying a theoretical foundation for its clinical application of its "same treatment for different diseases".
Influence of Digital Media in the Oral Health Education of Mother-Child Pairs: Study Protocol of a Parallel Double-Blind Randomized Clinical Trial
Authors: Ribeiro Yuri Jivago Silva , Ferreira Luanna Goncalves , filho Paulo Nelson , Arnez Maya Fernanda Manfrin , Paula-Silva Francisco Wanderley Garcia 
Published: March 2022

Abstract
Background
As a result of the COVID-19 pandemic and the growing use of smartphones, opportunities for using digital media applications to promote health and favorable behaviors have increased. Digital methods are among those and could be used for communication between physicians and patients including text messages, images, audio, and video calls. This study aimed to investigate the use of the smartphone application (WhatsApp) to deliver oral health education to mother-child pairs, with the ultimate goal of controlling dental biofilm and caries through digital activities focused on oral hygiene.
Methods
This study was designed as a randomized, double-blind, parallel clinical trial involving 100 pairs of mothers and children (6–12 years old). The mothers and children will be randomly allocated to the control group (n = 50 pairs), who will receive a single visit conventional oral health education, or to the experimental group (n = 50 pairs), who will receive both a single visit conventional oral health education and educational videos through WhatsApp Messenger, twice a week. Before randomization of the groups and after the intervention, pairs will be evaluated regarding to plaque index (VPI), gingival bleeding index (GBI), and number of decayed, missing and filled permanent or primary teeth (DMF-T) modified by the inclusion of active non-cavitated carious lesions (Nyvad criteria). Socioeconomic data, dental history and oral health literacy will obtained using questionnaires (Oral Health Literacy Assessment Task for Paediatric Dentistry; BOHLAT-P). Chi-square, Student’s t-test, paired Student’s t-test, Mann-Whitney, and Wilcoxon’s sign tests were used with a 5% significance level.
Discussion
This intervention proposal is designed to motivate behavioral change in mother-child pairs. We hypothesize that adding digital media to traditional oral health programs will provoke improvements in oral hygiene behavior and health outcomes. To our knowledge, this is the first study evaluating the effect of educational videos communicated by digital media (WhatsApp) on the oral health of mother-child pairs evaluated by long-term dental examinations. In addition, we will assess the maternal level of comprehension of the provided information via a literacy assessment tool. The clinical trial is registered at the Brazilian Registry of Clinical Trials (No. RBR-7s8bw6m).
A nomogram for predicting overall survival of patients with pancreatic ductal adenocarcinoma (PDAC) who underwent radical pancreatoduodenectomy(R0)
Authors: Liang Yurong , Cui Chenghao , Qin Tao , Hong Chenyan , Wang Yanwei , Li Mingtai , Cai Shouwang , Yu Qiang 
Published: March 2022

Abstract
Background: Hypercoagulation and malnutrition are characteristic pathophysiological changes of PDAC, which are intimately associated with cancer progression and prognosis. We aimed at integrating related indicators to build a nomogram model for providing individualized overall survival predictions for patients with PDAC who underwent radical pancreatoduodenectomy(R0) Method: Clinicopathological and survival data of 138 patients who met the inclusion criteria were retrospectively analyzed. A nomogram was built based on the regression formula of multivariate Cox analysis. calibration curves and the areas under the ROC curves (AUCs) were utilized to evaluate the predictive performance of the nomogram.Result: High-grade (HR:3.70; 95%CI: 1.51-3.82; P<0.001), vessel carcinoma embolus(HR:2.69; 95%CI: 1.30-5.31,P=0.007), N2(HR:2.90; 95%CI: 1.47-7.37; P=0.004), anemia(HR:1.98;95%CI:1.01-2.70; p=0.047), PLR>244.8(HR:2.13; 95%CI:1.05-3.45; P=0.033), FBG>3.50g/L(HR:2.10;95%CI: 1.04-3.09,P=0.008), DRR>1.1(HR:2.69; 95%CI:1.56-4.27 ;P<0.001) served as independent risk factors of poor overall survival of PDAC patients who underwent radical pancreatoduodenectomy(R0) .A nomogram model was constructed based on this result. The calibration curves fitted well to the ideal 45° line ，and the AUCs at 1 ,2 and 3 years were 0.713，0.777, 0.845 respectively.Conclusion: The nomogram established based on characteristic pathophysiological alterations of PDAC for the prediction of overall survival of patients who underwent radical pancreatoduodenectomy(R0) presented considerable predictive power and may facilitate prognostic risk stratification, optimizing therapeutic decision-making.
Women’s Awareness of Ovarian Cancer Risk Factors and Symptoms in Western Iran in 2020-2021
Authors: Babaei Maryam , Salmani Fatemeh , Kariman Nourossadat , Nasiri Saeideh , Ozgoli Giti 
Published: March 2022

Abstract
Objectives: This study aimed to investigate Iranian women’s awareness of warning signs and risk factors for ovarian cancer.Methods: This cross-sectional, descriptive study was conducted in Sanandaj and Kermanshah, Iran, in 2020 and 2021. In this study, 1081 women aged 18-70 years were selected as the sample to fill out the electronic version of the Ovarian Cancer Awareness Measure (OCAM) to identify the significant variables of ovarian cancer warning signs and risk factors. The obtained data were statistically analyzed by descriptive statistics and ordinal logistic regression in SPSS 19. Results: In this study, 60.9% of the participants had medium awareness of the subject. The results indicated that participants with higher educational attainment (P<0.05) and those with a family history of cancer (P<0.001) showed higher knowledge of cancer. The lowest level of awareness of symptoms was associated with acute symptoms such as dysphagia (swallowing problems) most days, persistent bloating, a sense of abdominal fullness or heaviness, and frequent urination. Moreover, the lowest awareness of risk factors was related to the history of IVF treatments and the application of talcum powder to the genital area.Conclusion: The study findings showed that Iranian women have moderate awareness of ovarian cancer and low knowledge on cancer warning signs; this suggests that it is necessary to train Iranian women in order to raise awareness of the signs and risk factors for ovarian cancer.
An impact analysis of COVID-19 on the relation between open innovation, IP openness and firm performance
Authors: Kanama Daisuke 
Published: March 2022

Abstract
This study empirically investigated how pre-COVID innovation activities and IP management affected business performance after the arrival of COVID-19. As a result, the following three points were clarified: First, the high level of R&D investment before the arrival of COVID-19 weakened the damage to sales and operating profit after its arrival. Second, we found that the firms that launched new products and services four years before the arrival of COVID-19 suffered more damage from COVID-19. Third, although innovation activities are vulnerable to the crisis as a whole, there are cases in which firms have not lost or improved their performance even after the arrival of COVID-19. Specifically, it became clear that the damage caused by COVID-19 was lesser for collaborative innovations involving many firms than for innovations realized by single firms. This was also demonstrated from the perspective of open IP management.
Influence of aniline and antimony on micro-oxygen hydrolysis and acidification processes in printing and dyeing wastewater treatment
Authors: LIU HONG , Pang Zuzu , Ye Shiqing , Ding Ning 
Published: March 2022

Abstract
This experiment studied the degradation characteristics of aniline and antimony in printing and dyeing wastewater by the micro-oxygen hydrolysis acidification process and its influence on the removal of COD and ammonia nitrogen. Firstly, the study optimized the control factors of pH、dissolved oxygen (DO)、sludge concentration on the removal efficiency of COD and ammonia nitrogen in hydrolysis and acidification section. It is recommended that pH can be maintained at 6.5; low dissolved oxygen (0-0.5 mg/L) would help the conversion of nitrogen substances for subsequent treatment, the optimal treatment temperature was found to be 25℃, furthermore, it is recommended to control the sludge concentration at 4 mg/L during operation. Secondly, the impact of aniline and antimony on COD and nitrogen removal was explored. It was found that when the aniline concentration increased from 0.4 mg/L to 5.4 mg/L, the effluent COD concentration increased, with a rate of 96.5%, indicating that aniline was toxic to anaerobic sludge and significantly inhibited the COD degradation; while when the antimony concentration increased from 0.05 mg/L to 2.05 mg/L, the COD removal rate was only 2.9%, which was far lower than the COD removal rate of water samples without adding antimony. The decrease of anaerobic sludge concentration from 5.58 g/L to 3.44 g/L indicated that aniline and antimony have a strong inhibitory effect on the activity of anaerobic bacteria, and reversely affect the COD removal.