bluuebunny/crossref_metadata_2025_split huggingface dataset:
In‐the‐moment ratings on the Early Relational Health Screen: A pilot study of application in home visiting and primary care
Authors: Rosenblum Katherine L. , Riggs Jessica , Freeman Sarah , Shah Prachi E. , Muzik Maria ,   the Michigan Collaborative for Infant Mental Health Research
Published: May 2022

AbstractEarly infant‐parent interaction sets a critical foundation for young children's well‐being, and evidence regarding the protective role of secure early relationships has led to increased interest in effective screening and promotion of early relational health in pediatric primary care and home visiting settings. We report findings from two pilot studies conducted in the United States that describe the reliability and validity of a relational health screening tool, the Early Relational Health Screen (ERHS), implemented in two different contexts: an innovative model of relational health promotion in pediatric primary care (Study 1) and an Infant Mental Health Home Visiting (IMH‐HV) model (Study 2). Across both studies, a trained clinician rated the ERHS following real‐time observation of interaction (i.e., “in‐the‐moment” ratings). Reliability was assessed by comparing “in‐the‐moment” ERHS ratings to subsequent coding of the same interaction from video by an independent evaluator. In addition, Study 2 data permitted evaluation of the validity of “in‐the‐moment” ERHS ratings. Results from both studies indicated reliability of “in‐the‐moment” ERHS ratings. In addition, Study 2 clinician “in‐the‐moment” ratings were associated with maternal depression and ratings of child‐parent interaction derived from a separate observational task coded by independent evaluators using a different well‐validated research‐based measure. Discussion highlights the potential of the ERHS as a screening, promotion, and prevention tool that may be feasibly administered by providers across pediatric primary care and home visiting settings.
Investigating the drivers of cybersecurity enhancement in public organizations: The case of Jordan
Authors: Al‐ma'aitah Mohammad Atwah 
Published: May 2022

AbstractElectronic government systems are broadly recognized in the modern era as useful and valid means of delivering e‐services. As the importance and prevalence of e‐government services have grown, increasing attention has necessarily been placed on the cybersecurity of electronic programs. Multiple drivers have been identified as contributing to the enhancement of cybersecurity, however, there is limited empirical research confirming these drivers. The main aims of this study are to investigate the drivers of e‐government cybersecurity enhancement and determine the impact of cybersecurity on the effectiveness of e‐government systems. The study constructs were extracted from the human–organization–technology (HOT) theory and institutional theory. A total of 500 questionnaires were randomly distributed to information technology department staff members and e‐government officials in Jordanian ministries. The resulting data were analyzed using a structural equation modeling approach. The findings indicate that technical measures, the role of senior management, coercive pressures, and memetic pressures are important drivers for enhanced cybersecurity levels in governmental organizations. The results also show that enhancing cybersecurity levels in organizations promote the effectiveness of e‐government services. These conclusions offer researchers and practitioners insight as to the drivers of an enhanced cybersecurity and the importance of cybersecurity in e‐government as a whole.
Optimization of production and characterization of a recombinant soluble human Cripto‐1 protein inhibiting self‐renewal of cancer stem cells
Authors: Afify Said M. , Hassan Ghmkin , Nawara Hend M. , H Zahra Maram , Xu Yanning , Alam Md Jahangir , Saitoh Koichi , Mansour Hager , Abu Quora Hagar A. , Sheta Mona , Monzur Sadia , Du Juan , Oh Sue Young , Seno Akimasa , Salomon David S. , Seno Masaharu 
Published: May 2022

AbstractHuman Cripto‐1 is a member of the epidermal growth factor (EGF)‐Cripto‐FRL‐1‐Cryptic (CFC) family family and performs critical roles in cancer and various pathological and developmental processes. Recently we demonstrated that a soluble form of Cripto‐1 suppresses the self‐renewal and enhances the differentiation of cancer stem cells (CSCs). A functional form of soluble Cripto‐1 was found to be difficult to obtain because of the 12 cysteine residues in the protein which impairs the folding process. Here, we optimized the protocol for a T7 expression system, purification from inclusion bodies under denatured conditions refolding of a His‐tagged Cripto‐1 protein. A concentrations of 0.2−0.4 mM isopropyl β‐D‐1‐thiogalactopyranoside (IPTG) at 37°C was found to be the optimal concentration for Cripto‐1 expression while imidazole at 0.5 M was the optimum concentration to elute the Cripto‐1 protein from a Ni‐column in the smallest volume. Cation exchange column chromatography of the Cripto‐1 protein in the presence of 8 M urea exhibited sufficient elution profile at pH 5, which was more efficient at recovery. The recovery of the protein reached to more than 26.6% after refolding with arginine. The purified Cripto‐1 exhibited high affinity to the anti‐ALK‐4 antibody and suppressed sphere forming ability of CSCs at high dose and induced cell differentiation.
Autophagic flux inhibition, apoptosis, and mitochondrial dysfunction in bile acids‐induced impairment of human placental trophoblast
Authors: Yang Xi , Zhou Yulai , Li Huan , Song Fuzhen , Li Juan , Zhang Yong , Lin Yi , Zhang Huijuan , Fan Jianxia , Wu Weibin 
Published: May 2022

AbstractIntrahepatic cholestasis of pregnancy (ICP) is a common pregnancy‐specific disease, characterized by increased bile acid levels and adverse fetal outcomes. We previously reported excessive bile acids led to dysfunction of placental trophoblasts in ICP. However, the detailed mechanism is still unclear. Autophagy is fundamental process for protecting cell survival against adverse conditions. Here, we evaluated the effect of increased concentration of bile acids on autophagy in trophoblasts in vitro and in vivo. First, we demonstrated that the autophagy substrate p62/sequestosome‐1 was accumulated in placental tissues from patients with ICP and in human trophoblasts treated with hydrophobic bile acids, including chenodeoxycholic acid and deoxycholic acid. Furthermore, we found that treatment with hydrophobic bile acids impaired autophagic flux in both time‐ and concentration‐dependent manners, by suppressing the AMP‐activated protein kinase/unc‐51‐like kinase 1 autophagic signaling pathway. Notably, trophoblasts were prone to apoptotic cell death upon starvation along with bile‐acids treatment in vitro or in an ICP mouse model in vivo. Additionally, we revealed mitochondrial dysfunction was the predominant biological process in excessive bile acids induced trophoblast impairment under starvation by proteomic assay. Collectively, our study proposed a complex interaction of excessive bile acids induced autophagic flux, mitochondrial dysfunction, and cellular apoptosis in placental trophoblasts may play a critical role in the pathogenesis of ICP.
Magnetic phenolic resin core‐shell structure derived carbon microspheres for ultrafast magnetic solid‐phase extraction of triazine herbicides
Authors: Pan Hong , Gan Zushan , Hu Haoyun , Liu Cheng , Huang Yipeng , Ruan Guihua 
Published: May 2022

In this study, monodisperse magnetic carbon microspheres were successfully synthesized through the carbonization of phenolic resin encapsulated Fe3O4 core‐shell structures. The magnetic carbon microspheres showed high performance in ultrafast extraction and separation of trace triazine herbicides from environmental water samples. Under optimized conditions, both the adsorption and desorption processes could be achieved in 2 min, and the maximum adsorption capacity for simazine and prometryn were 387.6 and 448.5 μg/g. Coupled with high‐performance liquid chromatography‐ultraviolet detection technology, the detection limit of triazine herbicides was in the range of 0.30–0.41 ng/mL. The mean recoveries ranged from 81.44 to 91.03% with relative standard deviations lower than 7.47%. The excellent magnetic solid‐phase extraction performance indicates that magnetic carbon microspheres are promising candidate adsorbents for the fast analysis of environmental contaminants.
Nanoscaled PAMAM Dendrimer Spacer Improved the Photothermal‒Photodynamic Treatment Efficiency of Photosensitizer‐Decorated Confeito‐Like Gold Nanoparticles for Cancer Therapy
Authors: Saw Wen Shang , Anasamy Theebaa , Do Thu Thi Anh , Lee Hong Boon , Chee Chin Fei , Isci Umit , Misran Misni , Dumoulin Fabienne , Chong Wu Yi , Kiew Lik Voon , Imae Toyoko , Chung Lip Yong 
Published: May 2022

AbstractA critical factor in developing an efficient photosensitizer‐gold nanoparticle (PS‐AuNP) hybrid system with improved plasmonic photosensitization is to allocate a suitable space between AuNPs and PS. Poly(amidoamine) (PAMAM) dendrimer is selected as a spacer between the PS and confeito‐like gold nanoparticles (confeito‐AuNPs), providing the required distance (≈2.5‒22.5 nm) for plasmon‐enhanced singlet oxygen generation and heat production upon 638‐nm laser irradiation and increase the cellular internalization of the nanoconjugates. The loading of the PS, tetrakis(4‐carboxyphenyl) porphyrin (TCPP), and modified zinc phthalocyanine (ZnPc1) onto PAMAM‐confeito‐AuNPs demonstrate better in vitro cancer cell‐killing efficacy, as the combined photothermal‒photodynamic therapies (PTT‒PDTs) outperforms the single treatment modalities (PTT or PDT alone). These PS‐PAMAM‐confeito‐AuNPs also demonstrate higher phototoxicity than photosensitizers directly conjugated to confeito‐AuNPs (TCPP‐confeito‐AuNPs and ZnPc1‐confeito‐AuNPs) against all breast cancer cell lines tested (MDA‐MB‐231, MCF7, and 4T1). In the in vivo studies, TCPP‐PAMAM‐confeito‐AuNPs are biocompatible and exhibit a selective tumor accumulation effect, resulting in higher antitumor efficacy than free TCPP, PAMAM‐confeito‐AuNPs, and TCPP‐confeito‐AuNPs. In vitro and in vivo evaluations confirm PAMAM effectiveness in facilitating cellular uptake, plasmon‐enhanced singlet oxygen and heat generation. In summary, this study highlights the potential of integrating a PAMAM spacer in enhancing the plasmon effect‐based photothermal‒photodynamic anticancer treatment efficiency of PS‐decorated confeito‐AuNPs.
Nanomaterials Based on Functional Polymers for Sensitizing Cancer Radiotherapy
Authors: Lin Jinfeng , Yin Mingming , Liu Xiaoming , Meng Fanling , Luo Liang 
Published: May 2022

AbstractDespite being the mainstay treatment for many types of cancer in clinic, radiotherapy is undertaking great challenges in overcoming a series of limitations. Radiosensitizers are promising agents capable of depositing irradiation energy and generating free radicals to enhance the radiosensitivity of tumor cells. Combining radiosensitizers with functional polymer‐based nanomaterials holds great potential to improve biodistribution, circulation time, and stability in vivo. The derived polymeric nano‐radiosensitizers can significantly improve the efficiency of tumor targeting and radiotherapy, and reduce the side effect to healthy tissues. In this review, an overview of functional polymer‐based nanomaterials for radiosensitization in recent years is provided. Particular emphases are given to the action mechanisms, drug loading methods, targeting efficiencies, the impact on therapeutic effects, and biocompatibility of various radiosensitizing polymers, which are classified as polymeric micelles, dendrimers, polymeric nanospheres, nanoscale coordination polymers, polymersomes, and nanogels. The challenges and outlooks of polymeric nano‐radiosensitizers are also discussed.
Alzheimer's disease amyloidogenesis is linked to altered lower urinary tract physiology
Authors: Hardy Cara C. , Ramasamy Ramalakshmi , Rosenberg Dawn A. , Kuchel George A. , Yan Riqiang , Hu Xiangyou , Smith Phillip P. 
Published: May 2022

AbstractAimsWhile most Alzheimer's disease (AD) research emphasizes cognitive and behavioral abnormalities, lower urinary tract symptoms (LUTS) are observed in a third of AD patients, contributing to morbidity, poor quality of life, and need for institutionalization. Alzheimer's disease‐associated urinary dysfunction (ADUD) has been assumed to be due to cognitive decline alone. While mouse studies have suggested that bladder innervation and voiding behavior may be altered in AD models, technical challenges precluded voiding reflex assessments. This study seeks to establish a mouse model of ADUD, and it seeks to characterize the noncognitive sequelae involved in AD‐pathology associated alterations in the voiding reflex.MethodsHaving developed techniques permitting the assessment of bladder volume, pressure, and flow in mice, we now provide evidence of alterations in involuntary bladder control and increased response heterogeneity in a transgenic amyloidosis mouse model of AD using cystometry and tissue pharmacomyography. Tg‐APP/PS1DE9 (PA) mice and their wild‐type (WT) littermates (n = 6–8 per group) were used before plaque onset in the PA mice (4–6 months) and after plaque accumulation in the PA mice (8–10 months) in comparison to their WT control littermates.ResultsNovel findings include data suggestive of sphincteric discoordination, with pharmacological evidence of altered adrenergic mechanisms.ConclusionsTogether, these data highlight the importance of addressing noncognitive sequelae of AD and offer novel translational insights into the debilitating impact of AD on LUTS and incontinence.
Development and validation of a grading system for assessing muscle mass phenotype using mid‐upper arm muscle area and handgrip strength in patients with incurable cancer
Authors: Calixto‐Lima Larissa , Souza‐Silva Renata de , Oliveira Livia Costa de , Chaves, Gabriela Villaça , Wiegert Emanuelly Varea Maria 
Published: May 2022

AbstractBackgroundThis study aimed to develop and validate a distinct method to evaluate muscle mass phenotype in patients with incurable cancer based on a combination of mid‐upper arm muscle area (MUAMA) and handgrip strength (HGS).MethodsThis prospective cohort study was conducted with patients with incurable cancer who were enrolled at the palliative care unit of a cancer institute. The 1,660 patients were randomized into two data sets: training (70%; n = 1162), used to determine the muscle mass phenotype groups, derived from a combination of MUAMA and HGS cutoff points related to 180‐days mortality; and validation (30%; n = 498), used to evaluate the relationship of the proposed muscle phenotype grading system with performance status, body composition, nutrition status, and mortality.ResultsThe training data set resulted in three distinct groups formed by combining the cutoff points of MUAMA and HGS, with the best muscle mass phenotype being group 1, the group with any impairment of muscle mass being the 2, and the worst muscle mass phenotype being group 3. In the validation data set, lower performance status (both sexes p < 0.001), worse skeletal muscle index (both sexes p < 0.001), muscle radiodensity (men, p = 0.001; women, p = 0.008), and nutritional status (men, p = 0.003; women, p < 0.001) were observed as MUAMA and HGS values diminished. Patients in group 3 presented significantly higher risk of 180‐day mortality (both sexes p < 0.001).ConclusionThe muscle mass phenotype grading system proved to be able to identify patients with lower performance status, worse body composition measurements and nutritional status, and higher risk of death in 180 days.
Integrating the effects of sucrose intake on the brain and white adipose tissue: Could autophagy be a possible link?
Authors: Mattar Pamela , Toledo‐Valenzuela Lilian , Hernández‐Cáceres María Paz , Peña‐Oyarzún Daniel , Morselli Eugenia , Perez‐Leighton Claudio 
Published: May 2022

AbstractExcess dietary sucrose is associated with obesity and metabolic diseases. This relationship is driven by the malfunction of several cell types and tissues critical for the regulation of energy balance, including hypothalamic neurons and white adipose tissue (WAT). However, the mechanisms behind these effects of dietary sucrose are still unclear and might be independent of increased adiposity. Accumulating evidence has indicated that dysregulation of autophagy, a fundamental process for maintenance of cellular homeostasis, alters energy metabolism in hypothalamic neurons and WAT, but whether autophagy could mediate the detrimental effects of dietary sucrose on hypothalamic neurons and WAT that contribute to weight gain is a matter of debate. In this review, we examine the hypothesis that dysregulated autophagy in hypothalamic neurons and WAT is an adiposity‐independent effect of sucrose that contributes to increased body weight gain. We propose that excess dietary sucrose leads to autophagy unbalance in hypothalamic neurons and WAT, which increases caloric intake and body weight, favoring the emergence of obesity and metabolic diseases.
Adaptive dynamic programming for decentralized neuro‐control of nonlinear systems subject to mismatched interconnections
Authors: Tang Yuhong , Yang Xiong , Dong Na 
Published: May 2022

AbstractThis article presents a decentralized neuro‐control scheme for a class of continuous‐time nonlinear systems with mismatched interconnections through an optimal control method. The decentralized control problem of the studied nonlinear‐interconnected systems is transformed into a group of optimal control problems of auxiliary subsystems. First, the value functions of auxiliary subsystems are designed, which are related to the boundaries of interconnected terms. It is proved that, under certain conditions, the decentralized control consisting of optimal control policies of auxiliary subsystems can stabilize the entire interconnected system. Then, an adaptive dynamic programming algorithm is presented to solve the Hamilton–Jacobi–Bellman equations. Critic neural networks (NNs) are employed to approximate the value functions of auxiliary subsystems in order to acquire the optimal control policies. Moreover, the auxiliary subsystems' states and the critic NNs' weight estimation errors are proved to be uniformly ultimately bounded. Finally, a numerical example and an interconnected power system are provided to validate the proposed decentralized neuro‐control strategy.
Predicting fragment intensities and retention time of iTRAQ‐ and TMTPro‐labeled peptides with Prosit‐TMT
Authors: Gabriel Wassim , Giurcoiu Victor , Lautenbacher Ludwig , Wilhelm Mathias 
Published: May 2022

AbstractIsobaric labeling increases the throughput of proteomics by enabling the parallel identification and quantification of peptides and proteins. Over the past decades, a variety of isobaric tags have been developed allowing the multiplexed analysis of up to 18 samples. However, experiments utilizing such tags often exhibit reduced identification rates and thus show decreased analytical depth. Re‐scoring has been shown to rescue otherwise missed identifications but was not yet systematically applied on isobarically labeled data. Because iTRAQ 4/8‐plex and the recently released TMTpro 16/18‐plex share similar characteristics with TMT 6/10/11‐plex, we hypothesized that Prosit‐TMT, trained exclusively on 6/10/11‐plex labeled peptides, may be applicable to these isobaric labeling strategies as well. To investigate this, we re‐analyzed nine publicly available datasets covering iTRAQ and TMTpro labeling for samples with human and mouse origin. We highlight that Prosit‐TMT shows remarkably good performance when comparing experimentally acquired and predicted fragmentation spectra (R of 0.84 ‐ 0.9) and retention times (ΔRT95% of 3% ‐ 10% gradient time) of peptides. Furthermore, re‐scoring substantially increases the number of confidently identified spectra, peptides, and proteins.
Deciphering the role of the two conserved motifs of the ECF41 family σ factor in the autoregulation of its own promoter in Azospirillum brasilense Sp245
Authors: Pathak Ekta , Dubey Ashutosh Prakash , Singh Vijay Shankar , Mishra Rajeev , Tripathi Anil Kumar 
Published: May 2022

AbstractIn Azospirillum brasilense, an extra‐cytoplasmic function σ factor (RpoE10) shows the characteristic 119 amino acid long C‐terminal extension found in ECF41‐type σ factors, which possesses three conserved motifs (WLPEP, DGGGR, and NPDKV), one in the linker region between the σ2 and σ4, and the other two in the SnoaL_2 domain of the C‐terminal extension. Here, we have described the role of the two conserved motifs in the SnoaL_2 domain of RpoE10 in the inhibition and activation of its activity, respectively. Truncation of the distal part of the C‐terminal sequence of the RpoE10 (including NPDKV but excluding the DGGGR motif) results in its promoter's activation suggesting autoregulation. Further truncation of the C‐terminal sequence up to its proximal part, including NPDKV and DGGGR motif, abolished promoter activation. Replacement of NPDKV motif with NAAAV in RpoE10 increased its ability to activate its promoter, whereas replacement of DGGGR motif led to reduced promoter activation. We have explored the dynamic modulation of σ2 ‐σ4 domains and the relevant molecular interactions mediated by the two conserved motifs of the SnoaL2 domain using molecular dynamics simulation. The analysis enabled us to explain that the NPDKV motif located distally in the C‐terminus negatively impacts transcriptional activation. In contrast, the DGGGR motif found proximally of the C‐terminal extension is required to activate RpoE10.
The utilities and pitfalls of stratified analysis in challenging situations
Authors: Wang Jin 
Published: May 2022

AbstractStratified analysis is commonly used to control confounding factors in evaluating treatment effect at both design and analysis stages. Motivated by a real‐life experience, this article illustrates the utilities and pitfalls of stratified analysis through continuous and binary endpoints by comparing some typical analysis methodologies. Stratified analyses in some challenging situations, including propensity score stratification are discussed.