bluuebunny/crossref_metadata_2025_split huggingface dataset:
To go left or right? Driving towards the best direction in paediatric pacing
Authors: Silvetti Massimo Stefano , Porco Luigina , Tamburri Ilaria , Campisi Marta , Gimigliano Fabrizio , Saputo Fabio Anselmo , Mizzon Chiara , Di Mambro Corrado , Albanese Sonia , Ravà Lucilla , Drago Fabrizio 
Published: June 2022

AbstractBackground:Permanent pacing in children with isolated congenital complete atrioventricular block may cause left ventricular dysfunction. To prevent it, alternative pacing sites have been proposed: left ventricular epicardial or selective right ventricular endocardial pacing.Aims:To compare the functional outcome (left ventricular systolic function and synchrony) in paediatric patients with congenital complete atrioventricular block and left ventricular apical epicardial or right ventricular transvenous mid-septal pacing.Methods:Retrospective study. Epicardial leads were implanted by standard surgical technique, transvenous leads by 3D electroanatomic mapping systems. 3D mapping acquired 3D right ventricular local pacing map and defined the narrowest paced QRS site. 3D mapping guided screw-in bipolar leads on that ventricular site. Electrocardiogram (ECG) (QRS duration) and echocardiographic data (synchrony: interventricular mechanical delay, septal to posterior wall motion delay, systolic dyssynchrony index; contractility: global longitudinal strain, ejection fraction) were recorded. Data are reported as median [interquartile ranges]. p < 0.05 was significant.Results:There were 19 transvenous systems (age 8.8 [6–14] years; right ventricular mid-septum) and 17 epicardial systems (0.04 [0.001–0.6] years; left ventricular apex). Post-implantation QRS significantly widened either in endocardial or in epicardial patients. Most patients reached 4-year follow-up. One-year and 4-year ejection fraction and global longitudinal strain were mostly within normal limits and did not show significant differences between the two groups and between the same endocardial/epicardial group. Synchrony parameters were within normal limits in the two groups.Conclusions:Left ventricular apical epicardial pacing and 3D mapping-guided right ventricular mid-septal pacing preserved left ventricular contractility and synchrony in children and adolescents with congenital complete atrioventricular block at short-/mid-term follow-up, without relevant significant differences between the two groups.
Affective forecasting in Parkinson’s disease
Authors: Coundouris Sarah P. , Henry Julie D. , Suddendorf Thomas , Lehn Alexander C. 
Published: June 2022

AbstractObjectives:While emotional responses experienced in-the-moment appear to remain intact in Parkinson’s disease (PD), no study has tested whether this extends to the prediction of future emotional responses. The present study aimed to provide the first assessment of affective forecasting capacity in this cohort.Methods:A positively and negatively valenced affective forecasting task and broader clinical battery were completed by a PD group (ns = 28 and 37, respectively) and a demographically matched neurotypical control group (ns = 38 and 39, respectively).Results:No group differences emerged on the two tasks, with the two groups underestimating their level of happiness and overestimating their level of negative affect to a similar degree. Affective forecasting error scores were unrelated to clinical characteristics.Conclusions:Given that affective forecasting relies on self-projection into the future, a skill shown to often be disrupted in this cohort, impairments were expected. However, this study provides initial evidence that this may not be the case. These findings are potentially important given that how we think about and envisage the future affectively is a major determinant of goal-directed behavior. Further work is now needed to establish whether these findings are robust and generalize to other types of affective stimuli.
PRESCRIBED VIRTUAL HOMOLOGICAL TORSION OF 3-MANIFOLDS
Authors: Chu Michelle , Groves Daniel 
Published: June 2022

AbstractLet M be an irreducible
$3$
-manifold M with empty or toroidal boundary which has at least one hyperbolic piece in its geometric decomposition, and let A be a finite abelian group. Generalizing work of Sun [20] and of Friedl–Herrmann [7], we prove that there exists a finite cover
$M' \to M$
so that A is a direct factor in
$H_1(M',{\mathbb Z})$
.
Design and fabrication of an inverted hat antenna paired with a filled cavity for radio-navigation applications
Authors: Hamouda Z. , Azaizia S. , Hideche M. , Zemmam A. , Lasri T. 
Published: June 2022

AbstractAntennas installed on aircraft are used for communications as well as for various radio navigation systems such as direction finders, distance measuring systems, and altimeters. Generally, these systems use blade antennas operating in the L frequency band. Recently, inverted-hat empty section monopole antennas have been found to be good candidates for such uses. In this study, we propose a new design of inverted-hat antenna based on optimized elliptical shapes and a filled cavity. The dielectric material added in the cavity helps to improve the monopole stability and to adjust the resonant frequency of the antenna. The proposed antenna meets the distance measuring equipment requirements, namely an omnidirectional radiation pattern in the H-plane, a vertical polarization, a frequency band from 960 MHz to 1.22 GHz, and a gain better than 1 dB. This antenna is entirely made of aluminum in order to obtain a homogeneity with the aluminum fuselage. In addition, the solution proposed brings a better protection against weather conditions. The antenna performance is analyzed on the basis of simulation and measurement results.
Ultra and Very Long Period Seismic Signatures of Unsteady Eruptions Predicted From Conduit Flow Models
Authors: Coppess Katherine R. , Dunham Eric M. , Almquist Martin 
Published: June 2022

AbstractExplosive volcanic eruptions radiate seismic waves as a consequence of pressure and shear traction changes within the conduit/chamber system. Kinematic source inversions utilize these waves to determine equivalent seismic force and moment tensor sources, but relation to eruptive processes is often ambiguous and nonunique. In this work, we provide an alternative, forward modeling approach to calculate moment tensor and force equivalents of a model of eruptive conduit flow and chamber depressurization. We explain the equivalence of two seismic force descriptions, the first in terms of traction changes on conduit/chamber walls, and the second in terms of changes in magma momentum, weight, and momentum transfer to the atmosphere. Eruption onset is marked by a downward seismic force, associated with loss of restraining shear tractions from fragmentation. This is followed by a much larger upward seismic force from upward drag of ascending magma and reduction of magma weight remaining in the conduit/chamber system. The static force is upward, arising from weight reduction. We calculate synthetic seismograms to examine the expression of eruptive processes at different receiver distances. Filtering these synthetics to the frequency band typically resolved by broadband seismometers produces waveforms similar to very long period seismic events observed in strombolian and vulcanian eruptions. However, filtering heavily distorts waveforms, accentuating processes in early, unsteady parts of eruptions and eliminating information about longer (ultra long period time scale depressurization and weight changes that dominate unfiltered seismograms. Our workflow can be utilized to directly and quantitatively connect eruption models with seismic observations.
Identification of nosZ-expressing microorganisms consuming trace N2O in microaerobic chemostat consortia dominated by an uncultured Burkholderiales
Authors: Kim Daehyun D , Han Heejoo , Yun Taeho , Song Min Joon , Terada Akihiko , Laureni Michele , Yoon Sukhwan 
Published: June 2022

Abstract
Microorganisms possessing N2O reductases (NosZ) are the only known environmental sink of N2O. While oxygen inhibition of NosZ activity is widely known, environments where N2O reduction occurs are often not devoid of O2. However, little is known regarding N2O reduction in microoxic systems. Here, 1.6-L chemostat cultures inoculated with activated sludge samples were sustained for ca. 100 days with low concentration (&lt;2 ppmv) and feed rate (&lt;1.44 µmoles h−1) of N2O, and the resulting microbial consortia were analyzed via quantitative PCR (qPCR) and metagenomic/metatranscriptomic analyses. Unintended but quantified intrusion of O2 sustained dissolved oxygen concentration above 4 µM; however, complete N2O reduction of influent N2O persisted throughout incubation. Metagenomic investigations indicated that the microbiomes were dominated by an uncultured taxon affiliated to Burkholderiales, and, along with the qPCR results, suggested coexistence of clade I and II N2O reducers. Contrastingly, metatranscriptomic nosZ pools were dominated by the Dechloromonas-like nosZ subclade, suggesting the importance of the microorganisms possessing this nosZ subclade in reduction of trace N2O. Further, co-expression of nosZ and ccoNO/cydAB genes found in the metagenome-assembled genomes representing these putative N2O-reducers implies a survival strategy to maximize utilization of scarcely available electron acceptors in microoxic environmental niches.
Ascitic fluid shear stress in concert with hepatocyte growth factor drive stemness and chemoresistance of ovarian cancer cells via the c-Met-PI3K/Akt-miR-199a-3p signaling pathway
Authors: Hassan Ayon A. , Artemenko Margarita , Tang Maggie K. S. , Shi Zeyu , Chen Lin-Yu , Lai Hung-Cheng , Yang Zhenyu , Shum Ho-Cheung , Wong Alice S. T. 
Published: June 2022

AbstractOvercoming drug resistance is an inevitable challenge to the success of cancer treatment. Recently, in ovarian cancer, a highly chemoresistant tumor, we demonstrated an important role of shear stress in stem-like phenotype and chemoresistance using a three-dimensional microfluidic device, which most closely mimics tumor behavior. Here, we examined a new mechanosensitive microRNA—miR-199a-3p. Unlike most key microRNA biogenesis in static conditions, we found that Dicer, Drosha, and Exportin 5 were not involved in regulating miR-199a-3p under ascitic fluid shear stress (0.02 dynes/cm2). We further showed that hepatocyte growth factor (HGF), but not other ascitic cytokines/growth factors such as epidermal growth factor and tumor necrosis factor α or hypoxia, could transcriptionally downregulate miR-199a-3p through its primary transcript miR-199a-1 and not miR-199a-2. Shear stress in the presence of HGF resulted in a concerted effect via a specific c-Met/PI3K/Akt signaling axis through a positive feedback loop, thereby driving cancer stemness and drug resistance. We also showed that miR-199a-3p expression was inversely correlated with enhanced drug resistance properties in chemoresistant ovarian cancer lines. Patients with low miR-199a-3p expression were more resistant to platinum with a significantly poor prognosis. miR-199a-3p mimic significantly suppressed ovarian tumor metastasis and its co-targeting in combination with cisplatin or paclitaxel further decreased the peritoneal dissemination of ovarian cancer in mice. These findings unravel how biophysical and biochemical cues regulate miR-199a-3p and is important in chemoresistance. miR-199a-3p mimics may serve as a novel targeted therapy for effective chemosensitization.
Highly-efficient RuNi single-atom alloy catalysts toward chemoselective hydrogenation of nitroarenes
Authors: Liu Wei , Feng Haisong , Yang Yusen , Niu Yiming , Wang Lei , Yin Pan , Hong Song , Zhang Bingsen , Zhang Xin , Wei Min 
Published: June 2022

AbstractThe design and exploitation of high-performance catalysts have gained considerable attention in selective hydrogenation reactions, but remain a huge challenge. Herein, we report a RuNi single atom alloy (SAA) in which Ru single atoms are anchored onto Ni nanoparticle surface via Ru–Ni coordination accompanied with electron transfer from sub-surface Ni to Ru. The optimal catalyst 0.4% RuNi SAA exhibits simultaneously improved activity (TOF value: 4293 h–1) and chemoselectivity toward selective hydrogenation of 4-nitrostyrene to 4-aminostyrene (yield: >99%), which is, to the best of our knowledge, the highest level compared with reported heterogeneous catalysts. In situ experiments and theoretical calculations reveal that the Ru–Ni interfacial sites as intrinsic active centers facilitate the preferential cleavage of N–O bond with a decreased energy barrier by 0.28 eV. In addition, the Ru–Ni synergistic catalysis promotes the formation of intermediates (C8H7NO* and C8H7NOH*) and accelerates the rate-determining step (hydrogenation of C8H7NOH*).
Photo and copper dual catalysis for allene syntheses from propargylic derivatives via one-electron process
Authors: Liu Qi , Zheng Jian , Zhang Xue , Ma Shengming 
Published: June 2022

AbstractDifferent from the traditional two-electron oxidative addition-transmetalation-reductive elimination coupling strategy, visible light has been successfully integrated into transition metal-catalyzed coupling reaction of propargylic alcohol derivatives highly selectively forming allenenitriles: specifically speaking, visible light-mediated Cu-catalyzed cyanation of propargylic oxalates has been realized for the general, efficient, and exclusive syntheses of di-, tri, and tetra-substituted allenenitriles bearing various synthetically versatile functional groups. A set of mechanistic studies, including fluorescence quenching experiments, cyclic voltammetric measurements, radical trapping experiments, control experiments with different photocatalyst, and DFT calculation studies have proven that the current reaction proceeds via visible light-induced redox-neutral reductive quenching radical mechanism, which is a completely different approach as compared to the traditional transition metal-catalyzed two-electron oxidative addition processes.
Deciphering quantum fingerprints in electric conductance
Authors: Daimon Shunsuke , Tsunekawa Kakeru , Kawakami Shinji , Kikkawa Takashi , Ramos Rafael , Oyanagi Koichi , Ohtsuki Tomi , Saitoh Eiji 
Published: June 2022

AbstractWhen the electric conductance of a nano-sized metal is measured at low temperatures, it often exhibits complex but reproducible patterns as a function of external magnetic fields called quantum fingerprints in electric conductance. Such complex patterns are due to quantum–mechanical interference of conduction electrons; when thermal disturbance is feeble and coherence of the electrons extends all over the sample, the quantum interference pattern reflects microscopic structures, such as crystalline defects and the shape of the sample, giving rise to complicated interference. Although the interference pattern carries such microscopic information, it looks so random that it has not been analysed. Here we show that machine learning allows us to decipher quantum fingerprints; fingerprint patterns in magneto-conductance are shown to be transcribed into spatial images of electron wave function intensities (WIs) in a sample by using generative machine learning. The output WIs reveal quantum interference states of conduction electrons, as well as sample shapes. The present result augments the human ability to identify quantum states, and it should allow microscopy of quantum nanostructures in materials by making use of quantum fingerprints.
Control of CRK-RAC1 activity by the miR-1/206/133 miRNA family is essential for neuromuscular junction function
Authors: Klockner Ina , Schutt Christian , Gerhardt Theresa , Boettger Thomas , Braun Thomas 
Published: June 2022

AbstractFormation and maintenance of neuromuscular junctions (NMJs) are essential for skeletal muscle function, allowing voluntary movements and maintenance of the muscle tone, thereby preventing atrophy. Generation of NMJs depends on the interaction of motor neurons with skeletal muscle fibers, which initiates a cascade of regulatory events that is essential for patterning of acetylcholine receptor (AChR) clusters at specific sites of the sarcolemma. Here, we show that muscle-specific miRNAs of themiR-1/206/133family are crucial regulators of a signaling cascade comprising DOK7-CRK-RAC1, which is critical for stabilization and anchoring of postsynaptic AChRs during NMJ development and maintenance. We describe that posttranscriptional repression of CRK bymiR-1/206/133is essential for balanced activation of RAC1. Failure to adjust RAC1 activity severely compromises NMJ function, causing respiratory failure in neonates and neuromuscular symptoms in adult mice. We conclude thatmiR-1/206/133serve a specific function for NMJs but are dispensable for skeletal muscle development.
RNF43 G659fs is an oncogenic colorectal cancer mutation and sensitizes tumor cells to PI3K/mTOR inhibition
Authors: Fang Lishan , Ford-Roshon Dane , Russo Max , O’Brien Casey , Xiong Xiaozhe , Gurjao Carino , Grandclaudon Maximilien , Raghavan Srivatsan , Corsello Steven M. , Carr Steven A. , Udeshi Namrata D. , Berstler James , Sicinska Ewa , Ng Kimmie , Giannakis Marios 
Published: June 2022

AbstractThe RNF43_p.G659fs mutation occurs frequently in colorectal cancer, but its function remains poorly understood and there are no specific therapies directed against this alteration. In this study, we find that RNF43_p.G659fs promotes cell growth independent of Wnt signaling. We perform a drug repurposing library screen and discover that cells with RNF43_p.G659 mutations are selectively killed by inhibition of PI3K signaling. PI3K/mTOR inhibitors yield promising antitumor activity in RNF43659mut isogenic cell lines and xenograft models, as well as in patient-derived organoids harboring RNF43_p.G659fs mutations. We find that RNF43659mut binds p85 leading to increased PI3K signaling through p85 ubiquitination and degradation. Additionally, RNA-sequencing of RNF43659mut isogenic cells reveals decreased interferon response gene expression, that is reversed by PI3K/mTOR inhibition, suggesting that RNF43659mut may alter tumor immunity. Our findings suggest a therapeutic application for PI3K/mTOR inhibitors in treating RNF43_p.G659fs mutant cancers.
Paramagnetic encoding of molecules
Authors: Kretschmer Jan , David Tomáš , Dračínský Martin , Socha Ondřej , Jirak Daniel , Vít Martin , Jurok Radek , Kuchař Martin , Císařová Ivana , Polasek Miloslav 
Published: June 2022

AbstractContactless digital tags are increasingly penetrating into many areas of human activities. Digitalization of our environment requires an ever growing number of objects to be identified and tracked with machine-readable labels. Molecules offer immense potential to serve for this purpose, but our ability to write, read, and communicate molecular code with current technology remains limited. Here we show that magnetic patterns can be synthetically encoded into stable molecular scaffolds with paramagnetic lanthanide ions to write digital code into molecules and their mixtures. Owing to the directional character of magnetic susceptibility tensors, each sequence of lanthanides built into one molecule produces a unique magnetic outcome. Multiplexing of the encoded molecules provides a high number of codes that grows double-exponentially with the number of available paramagnetic ions. The codes are readable by nuclear magnetic resonance in the radiofrequency (RF) spectrum, analogously to the macroscopic technology of RF identification. A prototype molecular system capable of 16-bit (65,535 codes) encoding is presented. Future optimized systems can conceivably provide 64-bit (~10^19 codes) or higher encoding to cover the labelling needs in drug discovery, anti-counterfeiting and other areas.
Efficient aqueous remote loading of peptides in poly(lactic-co-glycolic acid)
Authors: Giles Morgan B. , Hong Justin K. Y. , Liu Yayuan , Tang Jie , Li Tinghui , Beig Avital , Schwendeman Anna , Schwendeman Steven P. 
Published: June 2022

AbstractPoly(lactic-co-glycolic acid) (PLGA) long-acting release depots are effective for extending the duration of action of peptide drugs. We describe efficient organic-solvent-free remote encapsulation based on the capacity of common uncapped PLGA to bind and absorb into the polymer phase net positively charged peptides from aqueous solution after short exposure at modest temperature. Leuprolide encapsulated by this approach in low-molecular-weight PLGA 75/25 microspheres slowly and continuously released peptide for over 56 days in vitro and suppressed testosterone production in rats in an equivalent manner as the 1-month Lupron Depot®. The technique is generalizable to encapsulate a number of net cationic peptides of various size, including octreotide, with competitive loading and encapsulation efficiencies to traditional methods. In certain cases, in vitro and in vivo performance of remote-loaded PLGA microspheres exceeded that relative to marketed products. Remote absorption encapsulation further removes the need for a critical organic solvent removal step after encapsulation, allowing for simple and cost-effective sterilization of the drug-free microspheres before encapsulation of the peptide.
Short- and long-read metagenomics expand individualized structural variations in gut microbiomes
Authors: Chen Liang , Zhao Na , Cao Jiabao , Liu Xiaolin , Xu Jiayue , Ma Yue , Yu Ying , Zhang Xuan , Zhang Wenhui , Guan Xiangyu , Yu Xiaotong , Liu Zhipeng , Fan Yanqun , Wang Yang , Liang Fan , Wang Depeng , Zhao Linhua , Song Moshi , Wang Jun 
Published: June 2022

AbstractIn-depth profiling of genetic variations in the gut microbiome is highly desired for understanding its functionality and impacts on host health and disease. Here, by harnessing the long read advantage provided by Oxford Nanopore Technology (ONT), we characterize fine-scale genetic variations of structural variations (SVs) in hundreds of gut microbiomes from healthy humans. ONT long reads dramatically improve the quality of metagenomic assemblies, enable reliable detection of a large, expanded set of structural variation types (notably including large insertions and inversions). We find SVs are highly distinct between individuals and stable within an individual, representing gut microbiome fingerprints that shape strain-level differentiations in function within species, complicating the associations to metabolites and host phenotypes such as blood glucose. In summary, our study strongly emphasizes that incorporating ONT reads into metagenomic analyses expands the detection scope of genetic variations, enables profiling strain-level variations in gut microbiome, and their intricate correlations with metabolome.
Computational identification of HCV neutralizing antibodies with a common HCDR3 disulfide bond motif in the antibody repertoires of infected individuals
Authors: Bozhanova Nina G. , Flyak Andrew I. , Brown Benjamin P. , Ruiz Stormy E. , Salas Jordan , Rho Semi , Bombardi Robin G. , Myers Luke , Soto Cinque , Bailey Justin R. , Crowe James E. , Bjorkman Pamela J. , Meiler Jens 
Published: June 2022

AbstractDespite recent success in hepatitis C virus (HCV) treatment using antivirals, an HCV vaccine is still needed to prevent reinfections in treated patients, to avert the emergence of drug-resistant strains, and to provide protection for people with no access to the antiviral therapeutics. The early production of broadly neutralizing antibodies (bNAbs) associates with HCV clearance. Several potent bNAbs bind a conserved HCV glycoprotein E2 epitope using an unusual heavy chain complementarity determining region 3 (HCDR3) containing an intra-loop disulfide bond. Isolation of additional structurally-homologous bNAbs would facilitate the recognition of key determinants of such bNAbs and guide rational vaccine design. Here we report the identification of new antibodies containing an HCDR3 disulfide bond motif using computational screening with the Rosetta software. Using the newly-discovered and already-known members of this antibody family, we review the required HCDR3 amino acid composition and propose determinants for the bent versus straight HCDR3 loop conformation observed in these antibodies.