bluuebunny/crossref_metadata_2025_split huggingface dataset:
Economic burden of multiple sclerosis: a cross-sectional study in Iran
Authors: Rezaee Mehdi , Keshavarz Khosro , Izadi Sadegh , Jafari Abdosaleh , Ravangard Ramin 
Published: January 2022

Abstract
Background
Multiple Sclerosis (MS) is a chronic debilitating disease that imposes a heavy socioeconomic burden on societies. This study aimed to determine the economic burden of MS on patients using the first (CinnoVex and ReciGen) and second (Fingolimod and Natalizumab) drug therapy lines.

Methods
This cost of illness study was an economic evaluation carried out as cross-sectional research in 2019 in southern Iran. A total of 259 patients were enrolled in two lines of drug therapy (178 patients in the first line and 81 ones in the second). The prevalence-based approach and the bottom-up approach were used to collect cost information and to calculate the costs from the societal perspective, respectively. The human capital approach was applied to calculate indirect costs. To collect the required data a researcher-made data collection form was utilized. The data were obtained using the information available in the patients’ medical records and insurance invoices as well as their self-reports or that of their companions.

Results
The results showed that the annual costs of MS in the first and second lines of drug therapy per patient were $ 1919 and $ 4082 purchasing power parity (PPP), respectively, and in total, $ 2721 PPP in 2019. The highest mean costs in both lines were those of direct medical costs, of which purchasing the main medicines in both lines accounted for the highest.

Conclusion
Considering the findings of this study and in order to reduce the burden of the disease, the following suggestions are presented: providing necessary facilities for the production of MS drugs in the country; proper and equitable distribution of neurologists; expanding the provision of home care services; and using the technologies related to the Internet, including WhatsApp, to follow up the MS patients’ treatment.
The interplay of autophagy and oxidative stress in the pathogenesis and therapy of retinal degenerative diseases
Authors: Chang Kun-Che , Liu Pei-Feng , Chang Chia-Hsuan , Lin Ying-Cheng , Chen Yen-Ju , Shu Chih-Wen 
Published: January 2022

AbstractOxidative stress is mainly caused by intracellular reactive oxygen species (ROS) production, which is highly associated with normal physiological homeostasis and the pathogenesis of diseases, particularly ocular diseases. Autophagy is a self-clearance pathway that removes oxidized cellular components and regulates cellular ROS levels. ROS can modulate autophagy activity through transcriptional and posttranslational mechanisms. Autophagy further triggers transcription factor activation and degrades impaired organelles and proteins to eliminate excessive ROS in cells. Thus, autophagy may play an antioxidant role in protecting ocular cells from oxidative stress. Nevertheless, excessive autophagy may cause autophagic cell death. In this review, we summarize the mechanisms of interaction between ROS and autophagy and their roles in the pathogenesis of several ocular diseases, including glaucoma, age-related macular degeneration (AMD), diabetic retinopathy (DR), and optic nerve atrophy, which are major causes of blindness. The autophagy modulators used to treat ocular diseases are further discussed. The findings of the studies reviewed here might shed light on the development and use of autophagy modulators for the future treatment of ocular diseases.
Generalized fractal Jensen and Jensen–Mercer inequalities for harmonic convex function with applications
Authors: Butt Saad Ihsan , Agarwal Praveen , Yousaf Saba , Guirao Juan L. G. 
Published: January 2022

AbstractIn this paper, we present a generalized Jensen-type inequality for generalized harmonically convex function on the fractal sets, and a generalized Jensen–Mercer inequality involving local fractional integrals is obtained. Moreover, we establish some generalized Jensen–Mercer-type local fractional integral inequalities for harmonically convex function. Also, we obtain some generalized related results using these inequalities on the fractal space. Finally, we give applications of generalized means and probability density function.
Weighted variable Morrey–Herz estimates for fractional Hardy operators
Authors: Asim Muhammad , Hussain Amjad , Sarfraz Naqash 
Published: January 2022

AbstractThe present article discusses the boundedness criteria for the fractional Hardy operators on weighted variable exponent Morrey–Herz spaces ${M\dot{K}^{\alpha(\cdot),\lambda}_{q,p(\cdot)}(w)}$
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Scalability resilience framework using application-level fault injection for cloud-based software services
Authors: Al-Said Ahmad Amro , Andras Peter 
Published: January 2022

AbstractThis paper presents an investigation into the effect of faults on the scalability resilience of cloud-based software services. The study introduces an experimental framework using the Application-Level Fault Injection (ALFI) to investigate how the faults at the application level affect the scalability resilience and behaviour of cloud-based software services. Previous studies on scalability analysis of cloud-based software services provide a baseline of the scalability behaviour of such services, allowing to conduct in-depth scalability investigation of these services. Experimental analysis on the EC2 cloud using a real-world cloud-based software service is used to demonstrate the framework, considering delay latency of software faults with two varied settings and two demand scenarios. The experimental approach is explained in detail. Here we simulate delay latency injection with two different times, 800 and 1600 ms, and compare the results with the baseline data. The results show that the proposed approach allows a fair assessment of the fault scenario’s impact on the cloud software service’s scalability resilience. We explain the use of the methodology to determine the impact of injected faults on the scalability behaviour and resilience of cloud-based software services.
The prevalence and psychological relation of problem shopping: data from a large-scale sample from Turkey
Authors: Ünübol Başak , Ünsalver Barış Önen , Ünübol Hüseyin , Sayar Gökben Hızlı 
Published: January 2022

Abstract
Background
The purpose of the present study was to comprehensively examine the measurement aspects, the prevalence, and the psychological correlates of problem shopping among a large-scale national sample of Turkish adults.

Result
Participants (N = 24,380, 50% men, M age = 31.79 years, age range = 18–81 years) completed a questionnaire that comprised the Shopping Addiction Risk Questionnaire, the Brief Symptom Inventory, the Positive and Negative Affect Schedule, and the Experiences in Close Relationships-Revised. Results showed that 1.8% of the participants had probable shopping addiction. Being female, being younger, psychiatric distress, positive affect, negative affect, anxious attachment, and avoidant attachment were positive correlates of problem shopping.

Conclusion
The results of this large sample size study suggest that shopping addiction is not a rare condition in Turkey. Further research is needed to understand different motives that underlie the problematic shopping behavior in the young and female population in comparison to older and male populations. Preventive programs or any interventions for people with PSB needs to address regulation difficulties and development of healthy strategies to cope with psychiatric distress.
Patterns of handgun divestment among handgun owners in California
Authors: Swanson Sonja A. , Miller Matthew , Zhang Yifan , Prince Lea , Holsinger Erin E. , Templeton Zachary , Studdert David M. 
Published: January 2022

Abstract
Background
Little is known about voluntary divestment of firearms among US firearm owners. Here, we aim to estimate the proportion of handgun owners who divest their handguns in the years following their initial acquisition; examine the timing, duration, and dynamics of those divestments; and describe characteristics of those who divest.

Methods
We use data from the Longitudinal Study of Handgun Ownership and Transfer, a cohort of registered voters in California with detailed information on 626,756 adults who became handgun owners during the 12-year study period, 2004–2016. For the current study, persons were followed from the time of their initial handgun acquisition until divestment, loss to follow-up, death, or the end of the study period. We describe the cumulative proportion who divest overall and by personal and area-level characteristics. We also estimate the proportion who reacquired handguns among persons who divested.

Results
Overall, 4.5% (95% CI 4.5–4.6) of handgun owners divested within 5 years of their first acquisition, with divestment relatively more common among women and among younger adults. Among those who divested, 36.6% (95% CI 35.8–37.5) reacquired a handgun within 5 years.

Conclusions
Handgun divestment is rare, with the vast majority of new handgun owners retaining them for years.
Endurance running during late murine adolescence results in a stronger anterior cruciate ligament and flatter posterior tibial slopes compared to controls
Authors: Ochocki Danielle N. , Loflin Benjamin E. , Ahn Taeyong , Colglazier Kaitlyn A. , Young Andrew R. , Snider Anna A. , Bueckers Elizabeth P. , Wojtys Edward M. , Schlecht Stephen H. 
Published: January 2022

Abstract
Background
Anterior cruciate ligament (ACL) injury rates continue to rise among youth involved in recreational and competitive athletics, requiring a better understanding of how the knee structurally and mechanically responds to activity during musculoskeletal growth. Little is understood about how anatomical risk factors for ACL injury (e.g., small ACL size, narrow intercondylar notch, and steep posterior tibial slope) develop and respond to increased physical activity throughout growth. We hypothesized that the ACL-complex of mice engaged in moderate to strenuous physical activity (i.e., endurance running) throughout late adolescence and young adulthood would positively functionally adapt to repetitive load perturbations.

Methods
Female C57BL6/J mice (8 weeks of age) were either provided free access to a standard cage wheel with added resistance (n = 18) or normal cage activity (n = 18), for a duration of 4 weeks. Daily distance ran, weekly body and food weights, and pre- and post-study body composition measures were recorded. At study completion, muscle weights, three-dimensional knee morphology, ACL cross-sectional area, and ACL mechanical properties of runners and nonrunners were quantified. Statistical comparisons between runners and nonrunners were assessed using a two-way analysis of variance and a Tukey multiple comparisons test, with body weight included as a covariate.

Results
Runners had larger quadriceps (p = 0.02) and gastrocnemius (p = 0.05) muscles, but smaller hamstring (p = 0.05) muscles, compared to nonrunners. Though there was no significant difference in ACL size (p = 0.24), it was 13% stronger in runners (p = 0.03). Additionally, both the posterior medial and lateral tibial slopes were 1.2 to 2.2 degrees flatter than those of nonrunners (p < 0.01).

Conclusions
Positive functional adaptations of the knee joint to moderate to strenuous exercise in inbred mice offers hope that that some anatomical risk factors for ACL injury may be reduced through habitual physical activity. However, confirmation that a similar response to loading occurs in humans is needed.
Radiographic vs. MRI vs. arthroscopic assessment and grading of knee osteoarthritis - are we using appropriate imaging?
Authors: Newman Samuel , Ahmed Huzefah , Rehmatullah Nader 
Published: January 2022

AbstractPurposeRadiographs and MRI scans are commonly used imaging techniques in the assessment of knee osteoarthritis. However, it currently remains uncertain how good a representation of the actual condition of the knee joint these investigations provide. By comparing them against arthroscopic findings the aim of our study was to conclude how accurate these imaging techniques are at grading knee osteoarthritis.MethodsThis was a retrospective study looking at knee arthroscopies performed at a tertiary centre over a 5 year period. The Outerbridge grade given at arthroscopy was correlated with pre-operative radiograph and MRI scores, so as to assess the reliability of these imaging techniques at predicting the actual severity of knee osteoarthritis seen.ResultsKellgren-Lawrence (KL) grading of radiographs was moderately correlated with Outerbridge grades from arthroscopy for the medial compartment of the knee (Spearman’s rho (SR) 0.483,p < 0.001), with a milder correlation in the lateral compartment (SR 0.218,p = 0.003). MRI reporting of knee osteoarthritis was moderately correlated with Outerbridge grades in the medial compartment (SR 0.451,p < 0.001), mildly correlated for both the lateral (SR 0.299,p < 0.001) and patellofemoral joint compartments (SR 0.142,p = 0.054). KL and MRI grading was moderately correlated for the medial compartment (SR 0.475,p < 0.001) and mildly correlated for the lateral compartment (SR 0.277,p < 0.001).ConclusionThe ability of radiographs to represent the actual condition of knee osteoarthritis is underestimated. KL grading especially best represents the disease seen in the medial compartment of the knee joint, with a moderate correlation to Outerbridge scores given on arthroscopic assessment. We suggest that whilst MRI is a useful tool in the investigation of knee symptoms, it is often unnecessarily used in patients with OA, when in fact, radiographs alone would be sufficient.Evidence levelIII
Using VIIRS nightlights to estimate the impact of the 2015 Nepal earthquakes
Authors: Tveit Thomas , Skoufias Emmanuel , Strobl Eric 
Published: January 2022

AbstractWe use Visible Infrared Imaging Radiometer Suite (VIIRS) nightlight data to model the impact of the 2015 Nepal earthquakes. More specifically, the data—showing nightlight emissions—are used to examine the extent to which there is a difference in nightlight intensity between cells damaged in the earthquake versus undamaged cells based on (1) mean comparisons; and (2) fixed effect regression models akin to the double difference method. The analysis is carried out for the entire country as well as smaller regions in and around the Central area and Kathmandu, which were the hardest hit areas. Overall, the regressions find a significant and negative effect from the initial shock, followed by a positive net effect from aid and relief efforts, which is consistent with what one would expect to find. However, the mean analysis results are inconclusive and there is substantial noise in the nightlight measurements due to how the values are produced and persistent cloud cover over Nepal.
A physiotherapy-led transition to home intervention for older adults following emergency department discharge: protocol for a pilot feasibility randomised controlled trial
Authors: Conneely Mairéad , Leahy Aoife , O’Connor Margaret , Barry Louise , Corey Gillian , Griffin Anne , O’Shaughnessy Íde , O’Carroll Ida , Leahy Siobhán , Trépel Dominic , Ryan Damian , Robinson Katie , Galvin Rose 
Published: January 2022

Abstract
Background
Older adults frequently attend the emergency department (ED) and experience high rates of adverse outcomes following ED presentation including functional decline, ED re-presentation and unplanned hospital admission. The development of effective interventions to prevent such outcomes is a key priority for research and service provision. This paper reports a protocol designed to evaluate the feasibility of conducting a three arm randomised controlled trial (RCT) within the ED setting and in the patient’s home. The interventions are comprehensive geriatric assessment (CGA), ED PLUS and usual care.

Methods
The ED PLUS pilot trial is designed as a feasibility RCT conducted in the ED and Acute Medical Assessment Unit of a university teaching hospital in the mid-west region of Ireland. We aim to recruit 30 patients, aged 65 years and over presenting to the ED with undifferentiated medical complaints and discharged within 72 h of index visit.
Patients will be randomised by a computer in a ratio of 1:1:1 to deliver usual care, CGA or ED PLUS during a 6-month study period. A randomised algorithm is used to perform randomization. CGA will include a medical assessment, medication review, nursing assessment, falls assessment, assessment of mobility and stairs, transfers, personal care, activities of daily living (ADLs), social supports and baseline cognition. ED PLUS, a physiotherapist led, multidisciplinary intervention, aims to bridge the transition of care between the index visit to the ED and the community by initiating a CGA intervention in the ED and implementing a 6-week follow-up self-management programme in the patient’s own home following discharge from the ED. The outcomes will be parameters of the feasibility of the intervention and trial methods and will be assessed quantitatively and qualitatively.

Discussion
Rising ED visits and an ageing population with chronic health issues render ED interventions to reduce adverse outcomes in older adults a research priority. This feasibility RCT will generate data and experience to inform the conduct and delivery of a definite RCT.

Trial registration
The trial was registered in Clinical Trials Protocols and Results System as of 21st July 2021, with registration number NCT049836020.
Metagenomic and metatranscriptomic analyses reveal minor-yet-crucial roles of gut microbiome in deep-sea hydrothermal vent snail
Authors: Yang Yi , Sun Jin , Chen Chong , Zhou Yadong , Van Dover Cindy Lee , Wang Chunsheng , Qiu Jian-Wen , Qian Pei-Yuan 
Published: January 2022

Abstract
Background
Marine animals often exhibit complex symbiotic relationship with gut microbes to attain better use of the available resources. Many animals endemic to deep-sea chemosynthetic ecosystems host chemoautotrophic bacteria endocellularly, and they are thought to rely entirely on these symbionts for energy and nutrition. Numerous investigations have been conducted on the interdependence between these animal hosts and their chemoautotrophic symbionts. The provannid snail Alviniconcha marisindica from the Indian Ocean hydrothermal vent fields hosts a Campylobacterial endosymbiont in its gill. Unlike many other chemosymbiotic animals, the gut of A. marisindica is reduced but remains functional; yet the contribution of gut microbiomes and their interactions with the host remain poorly characterised.

Results
Metagenomic and metatranscriptomic analyses showed that the gut microbiome of A. marisindica plays key nutritional and metabolic roles. The composition and relative abundance of gut microbiota of A. marisindica were different from those of snails that do not depend on endosymbiosis. The relative abundance of microbial taxa was similar amongst three individuals of A. marisindica with significant inter-taxa correlations. These correlations suggest the potential for interactions between taxa that may influence community assembly and stability. Functional profiles of the gut microbiome revealed thousands of additional genes that assist in the use of vent-supplied inorganic compounds (autotrophic energy source), digest host-ingested organics (carbon source), and recycle the metabolic waste of the host. In addition, members of five taxonomic classes have the potential to form slime capsules to protect themselves from the host immune system, thereby contributing to homeostasis. Gut microbial ecology and its interplay with the host thus contribute to the nutritional and metabolic demands of A. marisindica.

Conclusions
The findings advance the understanding of how deep-sea chemosymbiotic animals use available resources through contributions from gut microbiota. Gut microbiota may be critical in the survival of invertebrate hosts with autotrophic endosymbionts in extreme environments.
Characterization of captive and wild 13-lined ground squirrel cecal microbiotas using Illumina-based sequencing
Authors: Chiang Edna , Deblois Courtney L. , Carey Hannah V. , Suen Garret 
Published: January 2022

AbstractBackgroundHibernating animals experience extreme changes in diet that make them useful systems for understanding host-microbial symbioses. However, most of our current knowledge about the hibernator gut microbiota is derived from studies using captive animals. Given that there are substantial differences between captive and wild environments, conclusions drawn from studies with captive hibernators may not reflect the gut microbiota’s role in the physiology of wild animals. To address this, we used Illumina-based sequencing of the 16S rRNA gene to compare the bacterial cecal microbiotas of captive and wild 13-lined ground squirrels (TLGS) in the summer. As the first study to use Illumina-based technology to compare the microbiotas of an obligate rodent hibernator across the year, we also reported changes in captive TLGS microbiotas in summer, winter, and spring.ResultsWild TLGS microbiotas had greater richness and phylogenetic diversity with less variation in beta diversity when compared to captive microbiotas. Taxa identified as core operational taxonomic units (OTUs) and found to significantly contribute to differences in beta diversity were primarily in the familiesLachnospiraceaeandRuminococcaceae. Captive TLGS microbiotas shared phyla and core OTUs across the year, but active season (summer and spring) microbiotas had different alpha and beta diversities than winter season microbiotas.ConclusionsThis is the first study to compare the microbiotas of captive and wild rodent hibernators. Our findings suggest that data from captive and wild ground squirrels should be interpreted separately due to their distinct microbiotas. Additionally, as the first study to compare seasonal microbiotas of obligate rodent hibernators using Illumina-based 16S rRNA sequencing, we reported changes in captive TLGS microbiotas that are consistent with previous work. Taken together, this study provides foundational information for improving the reproducibility and experimental design of future hibernation microbiota studies.