bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unravelling cucumber resistance to several viruses via genome-wide association studies highlighted resistance hotspots and new QTLs
Authors: Monnot Severine , Cantet Melissa , Mary-Huard Tristan , Moreau Laurence , Lowdon Rebecca , Van Haesendonck Maurine , Ricard Agnès , Boissot Nathalie 
Published: August 2022

Abstract
The mapping and introduction of sustainable resistance to viruses in crops is a major challenge in modern breeding, especially regarding vegetables. We hence assembled a panel of cucumber elite lines and landraces from different horticultural groups for testing with six virus species. We mapped 18 quantitative trait loci (QTL) with a multiloci genome wide association studies (GWAS), some of which have already been described in the literature. We detected two resistance hotspots, one on chromosome 5 for resistance to the cucumber mosaic virus (CMV), cucumber vein yellowing virus (CVYV), cucumber green mottle mosaic virus (CGMMV) and watermelon mosaic virus (WMV), colocalizing with the RDR1 gene, and another on chromosome 6 for resistance to the zucchini yellowing mosaic virus (ZYMV) and papaya ringspot virus (PRSV) close to the putative VPS4 gene location. We observed clear structuring of resistance among horticultural groups due to plant virus coevolution and modern breeding which have impacted linkage disequilibrium (LD) in resistance QTLs. The inclusion of genetic structure in GWAS models enhanced the GWAS accuracy in this study. The dissection of resistance hotspots by local LD and haplotype construction helped gain insight into the panel’s resistance introduction history. ZYMV and CMV resistance were both introduced from different donors in the panel, resulting in multiple resistant haplotypes at same locus for ZYMV, and in multiple resistant QTLs for CMV.
Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B
Authors: Wu Ze , Li Ting , Cao Xing , Zhang Dehua , Teng Nianjun 
Published: August 2022

Abstract
Most of WRKY transcription factors play important roles in plant development, protection against disease, and response to abiotic stress; however, their roles in lily are largely unknown. Transcriptome analysis in lily (Lilium longiflorum) led to the identification and isolation of a WRKY-IIe gene, LlWRKY22, which was found to be activated at high temperature and play a positive role in thermotolerance regulation. LlWRKY22 expression was continuously activated by heat stress. We further found that LlWRKY22 protein localized to the nucleus and exhibited transactivation activity in both yeast and plant cells, and that its C terminus contributed to its transactivation activity. Meanwhile, overexpression of LlWRKY22 in lily improved thermotolerance and activated the expression of heat-related LlDREB2B gene; however, silencing of LlWRKY22 exerted the opposite effects. Further analysis revealed that LlWRKY22 directly activated the expression of LlDREB2B by binding to two tandem W-box elements on its promoter. Simultaneously, we also found that LlWRKY22 can directly bind its own promoter, thereby activating its own expression and forming a positive regulatory loop. Combined, our findings demonstrated that LlWRKY22 may be a new regulator of heat stress response and positively participates in the establishment of thermotolerance by activating itself and LlDREB2B.
Vicarious Trauma Scale: Psychometric Properties in a Sample of Social Workers from Spain
Authors: Aguiar-Fernández Francisco J , Méndez-Fernández Ana B , Lombardero-Posada Xoán M , Murcia-Álvarez Evelia , González-Fernández Antonio 
Published: August 2022

Abstract
As a result of secondary exposure to traumatic material, social workers may experience vicarious trauma. However, the analysis of this variable among social workers is scarce. The Vicarious Trauma Scale (VTS) is a brief instrument designed to measure the stress consequence of shared trauma. This study aims to examine the psychometrics of the VTS in a sample of 448 social workers from Spain. The results from the exploratory and confirmatory factor analyses (EFA and CFA) indicated that the VTS has satisfactory psychometric properties. Different indices of internal consistency supported the reliability of the VTS. Both EFA and CFA revealed the existence of two factors, corresponding to the cognitive and affective consequences of secondary exposure to trauma. Finally, the correlations of the VTS with other relevant and well-known job variables (workload, work–family conflict, detachment, supervisor support, burnout, and engagement) followed the expected pattern, and the VTS differentiated the social workers by their trauma caseload. Therefore, the VTS can be considered an adequate screening method of social workers’ vicarious trauma, and its application recommended to examine the possible risk and protective factors and consequences.
Employment and innovation in recessions: firm-level evidence from European Countries
Authors: Peters Bettina , Dachs Bernhard , Hud Martin , Köhler Christian 
Published: August 2022

Abstract
This paper investigates the employment effects of innovation over the business cycle with a large data set of manufacturing firms from 26 European countries from 1998 to 2014. The paper reveals four important findings: First, the net employment effect of product innovation is pro-cyclical and positive except in recessions. Second, product innovators are more resilient in recessions than non-product innovators because they are able to substitute demand losses in old products with new products. As a result, the net employment losses of product innovators are significantly lower in recessions than those of non-product innovators. Third, we find resilience only among small and medium-sized enterprises (SMEs), but not among large firms. Fourth, process and organizational innovations displace labor primarily during upturn and downturn periods.
Frequency criteria for exponential stability
Authors: Gannot Oran 
Published: August 2022

Abstract
We discuss some frequency-domain criteria for the exponential stability of nonlinear feedback systems based on Popov’s hyperstability theorem. The main results are new bounds on convergence rates for perturbations of the damped harmonic oscillator, including the Hessian-damped case, when the perturbations satisfy certain sectoriality or convexity hypotheses. We also include a simplified proof of Popov’s theorem in its general form, which has the virtue that it often applies at the margins of stability when other criteria typically degenerate.
Scanning the medical phenome to identify new diagnoses after recovery from COVID-19 in a US cohort
Authors: Kerchberger Vern Eric , Peterson Josh F , Wei Wei-Qi 
Published: August 2022

Abstract

Objective
COVID-19 survivors are at risk for long-term health effects, but assessing the sequelae of COVID-19 at large scales is challenging. High-throughput methods to efficiently identify new medical problems arising after acute medical events using the electronic health record (EHR) could improve surveillance for long-term consequences of acute medical problems like COVID-19.

Materials and Methods
We augmented an existing high-throughput phenotyping method (PheWAS) to identify new diagnoses occurring after an acute temporal event in the EHR. We then used the temporal-informed phenotypes to assess development of new medical problems among COVID-19 survivors enrolled in an EHR cohort of adults tested for COVID-19 at Vanderbilt University Medical Center.

Results
The study cohort included 186 105 adults tested for COVID-19 from March 5, 2020 to November 1, 2021; of which 30 088 (16.2%) tested positive. Median follow-up after testing was 412 days (IQR 274–528). Our temporal-informed phenotyping was able to distinguish phenotype chapters based on chronicity of their constituent diagnoses. PheWAS with temporal-informed phenotypes identified increased risk for 43 diagnoses among COVID-19 survivors during outpatient follow-up, including multiple new respiratory, cardiovascular, neurological, and pregnancy-related conditions. Findings were robust to sensitivity analyses, and several phenotypic associations were supported by changes in outpatient vital signs or laboratory tests from the pretesting to postrecovery period.

Conclusion
Temporal-informed PheWAS identified new diagnoses affecting multiple organ systems among COVID-19 survivors. These findings can inform future efforts to enable longitudinal health surveillance for survivors of COVID-19 and other acute medical conditions using the EHR.
Effective plasmid delivery to a plasmid-free Bacillus natto strain by a conjugational transfer system
Authors: Itaya Mitsuhiro , Sato Mitsuru , Watanabe Satoru , Kataoka Masakazu 
Published: August 2022

Abstract
In this study, a Bacillus natto strain named NEST141 was constructed. The strain carries no plasmids and is an authentic proline auxotroph—a feature that confers effective selection conditions for plasmids transferred from a donor, such as Bacillus subtilis 168, via a pLS20-based conjugational transfer system. We have provided a standard effective protocol for the delivery of plasmids larger than 50 kilobase pairs. These results indicate that the B. natto NEST141 strain can become a standard model, like B. subtilis 168, for extensive genetic engineering with diverse applications.
Differential Vulnerability of Hippocampal Subfields in Primary Age-Related Tauopathy and Chronic Traumatic Encephalopathy
Authors: Farrell Kurt , Iida Megan A , Cherry Jonathan D , Casella Alicia , Stein Thor D , Bieniek Kevin F , Walker Jamie M , Richardson Timothy E , White Charles L , Alvarez Victor E , Huber Bertrand R , Dickson Dennis W , Insausti Ricardo , Dams-O'Connor Kristen ,   The Part Working Group, Vonsattel Jean-Paul , Teich Andy F , Gearing Marla , Glass Jonathan , Troncoso Juan C , Frosch Matthew P , Hyman Bradley T , Murray Melissa E , Attems Johannes , Flanagan Margaret E , Mao Qinwen , Mesulam M-Marsel , Weintraub Sandra , Woltjer Randy L , Pham Thao , Kofler Julia , Schneider Julie A , Yu Lei , Purohit Dushyant P , Haroutunian Vahram , Hof Patrick R , Gandy Sam , Sano Mary , Beach Thomas G , Poon Wayne , Kawas Claudia H , Corrada María M , Rissman Robert A , Metcalf Jeff , Shuldberg Sara , Salehi Bahar , Nelson Peter T , Trojanowski John Q , Lee Edward B , Wolk David A , McMillan Corey T , Keene C Dirk , Latimer Caitlin S , Montine Thomas J , Kovacs Gabor G , Lutz Mirjam I , Fischer Peter , Perrin Richard J , Cairns Nigel J , McKee Ann C , Crary John F 
Published: August 2022

Abstract
Chronic traumatic encephalopathy (CTE) is a tauopathy associated with repetitive mild head impacts characterized by perivascular hyperphosphorylated tau (p-tau) in neurofibrillary tangles (NFTs) and neurites in the depths of the neocortical sulci. In moderate to advanced CTE, NFTs accumulate in the hippocampus, potentially overlapping neuroanatomically with primary age-related tauopathy (PART), an age-related tauopathy characterized by Alzheimer disease-like tau pathology in the hippocampus devoid of amyloid plaques. We measured p-tau burden using positive-pixel counts on immunohistochemically stained and neuroanatomically segmented hippocampal tissue. Subjects with CTE had a higher total p-tau burden than PART subjects in all sectors (p = 0.005). Within groups, PART had significantly higher total p-tau burden in CA1/subiculum compared to CA3 (p = 0.02) and CA4 (p = 0.01) and total p-tau burden in CA2 trended higher than CA4 (p = 0.06). In CTE, total p-tau burden in CA1/subiculum was significantly higher than in the dentate gyrus; and CA2 also trended higher than dentate gyrus (p = 0.01, p = 0.06). When controlling for p-tau burden across the entire hippocampus, CA3 and CA4 had significantly higher p-tau burden in CTE than PART (p &lt; 0.0001). These data demonstrate differences in hippocampal p-tau burden and regional distribution in CTE compared to PART that might be helpful in differential diagnosis and reveal insights into disease pathogenesis.
Characterization of ‘QTL-hotspot’ introgression lines reveals physiological mechanisms and candidate genes associated with drought adaptation in chickpea
Authors: Barmukh Rutwik , Roorkiwal Manish , Dixit Girish P , Bajaj Prasad , Kholova Jana , Smith Millicent R , Chitikineni Annapurna , Bharadwaj Chellapilla , Sreeman Sheshshayee M , Rathore Abhishek , Tripathi Shailesh , Yasin Mohammad , Vijayakumar Adiveppa G , Rao Sagurthi Someswar , Siddique Kadambot H M , Varshney Rajeev K 
Published: August 2022

Abstract‘QTL-hotspot’ is a genomic region on linkage group 04 (CaLG04) in chickpea (Cicer arietinum) that harbours major-effect quantitative trait loci (QTLs) for multiple drought-adaptive traits, and it therefore represents a promising target for improving drought adaptation. To investigate the mechanisms underpinning the positive effects of ‘QTL-hotspot’ on seed yield under drought, we introgressed this region from the ICC 4958 genotype into five elite chickpea cultivars. The resulting introgression lines (ILs) and their parents were evaluated in multi-location field trials and semi-controlled conditions. The results showed that the ‘QTL-hotspot’ region improved seed yield under rainfed conditions by increasing seed weight, reducing the time to flowering, regulating traits related to canopy growth and early vigour, and enhancing transpiration efficiency. Whole-genome sequencing data analysis of the ILs and parents revealed four genes underlying the ‘QTL-hotspot’ region associated with drought adaptation. We validated diagnostic KASP markers closely linked to these genes using the ILs and their parents for future deployment in chickpea breeding programs. The CaTIFY4b-H2 haplotype of a potential candidate gene CaTIFY4b was identified as the superior haplotype for 100-seed weight. The candidate genes and superior haplotypes identified in this study have the potential to serve as direct targets for genetic manipulation and selection for chickpea improvement.
Autism Spectrum Disorder Versus Communication Disorders
Authors: Paul Rhea 
Published: August 2022

AbstractThis chapter discusses differential diagnosis among autism spectrum disorder (ASD), language disorder, and social pragmatic disorder, a newly recognized condition that affects social communication in the absence of other autistic syndromes, which was recently added to the Diagnostic and Statistical Manual of Mental Disorders (DSM). Issues of diagnostic accuracy and validity of the nonautistic forms of communication disorder, and the distinctions among them, are discussed. Primary differentiating characteristics of the three disorders, including presence of joint attention, use of gestures and pretend play, language comprehension and the presence of restricted and repetitive behaviors are detail. Differences in diagnostic procedures necessary for children over and under the age of 5 years are provided. For children under 5 the potential instability of language status is highlighted, and differential diagnosis at this age level is discussed, with contrasts to differential diagnostic considerations for school-aged children and adolescents drawn. A case study is presented to illustrate the principles of differential diagnosis, and a decision tree for conferring diagnoses among these communication disorders is proposed.
Autism Spectrum Disorder Versus Posttraumatic Stress Disorder
Authors: Saqui Sonja , Garvin Brigid , Kerns Connor M. 
Published: August 2022

AbstractThis chapter provides key information about trauma in childhood and explores how to assess and differentiate symptoms of autism spectrum disorder in children who have experienced trauma. Specifically, the chapter first reviews the literature regarding trauma in young and school-age children, including the challenges of differential diagnosis of trauma response and autism spectrum disorder. To clarify areas of symptom overlap with autism spectrum disorder, the differences between autism spectrum disorder and trauma response in early childhood and school-age children are then provided. Next, this chapter offers a framework for a systematic approach to navigating a differential diagnosis of autism spectrum disorder and trauma-related disorders. Finally, two case vignettes are provided to illustrate the process of differential diagnosis.
Clinical Decision-Making in Evidence-Based Assessment
Authors: Elias Rebecca , Lord Catherine 
Published: August 2022

Abstract
An evaluation for possible autism spectrum disorder (ASD) is a complex process, which necessitates surveying multiple informants and utilizing validated assessment tools. In this chapter, we recommend an iterative, multistep assessment process, which is informed by the needs, context, and concerns of the client and their family. In addition to assessing the core features of ASD, we highlight the importance of addressing potential externalizing and internalizing problems, cognitive functioning, age, and risk/protective factors to determine if co-occurring psychopathology is present. This is especially pertinent because co-occurring psychopathology can be equally limiting as ASD but is often more amenable to treatment than the core features of ASD themselves. Taken together, disentangling co-occurring diagnosis from a differential diagnosis is challenging but enhanced when using evidence-based assessment tools to create a clinical conceptualization.
Interdisciplinary Behavioral Health Teams
Authors: Patterson Chavis A. , Elgohail Mona , Hartman Alison R. , Smith Vincent C. , Geller Pamela A. 
Published: August 2022

Abstract
Advances in technology have allowed for more complex medical interventions at a much younger gestational age. With such advances in neonatal care, the age of viability has improved. Medical teams are now able to intervene in utero to identify, address, and correct certain congenital anomalies, malformations, and disruptions in the normal process of fetal development. Medicine is also embracing a more inclusive, patient- and family-centered approach to care. Multidisciplinary teams across fetal care, obstetrics, neonatal intensive care unit (NICU), and NICU follow-up clinic settings that offer emotional support to families have grown to include psychology, social work, child life specialists, and chaplains. In some hospitals, perinatal psychiatry is also available to caregivers who have an infant in the NICU. These disciplines take an active role in helping parents manage the perinatal mood and anxiety disorders that often accompany the stress that they experience, both while the fetus is in utero and postpartum.
Specialty Care Settings
Authors: Teefey Christina Paidas , Robinson Sarah , Moldenhauer Julie S. 
Published: August 2022

Abstract
The prenatal diagnosis and management of congenital malformations have evolved with technical advancement of commonly employed imaging modalities such as ultrasound, magnetic resonance imaging, and echocardiography. Unlike most aspects of health care that focus on a single patient, two patients are involved in these complex pregnancies—the pregnant woman and her unborn child. Fetal diagnoses often require close antepartum surveillance and coordinated delivery planning. In addition, fetal therapy can be beneficial in appropriately selected cases. Fetal therapy centers were developed in order to provide seamless maternal, fetal, and neonatal care. This spectrum of care includes medical, surgical, and psychosocial services. The role of obstetric providers in a fetal therapy center is to support a multidisciplinary comprehensive model of care throughout the course of the affected pregnancy, birth, and postpartum period.
Neonatal Intensive Care Settings
Authors: Handley Sara C. , Munson David A. 
Published: August 2022

Abstract
Intensive care units (ICUs) designed to provide cutting-edge care to infants have made considerable improvements in the care and outcomes during the past several decades. Nonetheless, congenital anomalies and complications of prematurity remain the leading causes of death in infancy. Parents must navigate difficult decision, sometimes emergently, and often overly long inpatient stays during which there can be significant and frequent changes in clinical status. Teams in the ICU must partner with parents to ensure clear understanding of the medical issues for effective shared decision-making. Psychosocial providers in the ICU provide a critical layer of support for families as they think through the meaning of illness for their child. This chapter familiarizes providers of psychosocial care in newborn infant ICUs or cardiac ICUs with terminology, structure, staffing, and some common diseases and complications.
Role of Behavioral Health Clinicians in the NICU Setting
Authors: Steinwurtzel Rochelle , Brachio Sandhya S. , Ho Sheau-Yan , Santiago-Warner Solimar 
Published: August 2022

Abstract
This chapter addresses the multifaceted role of the behavioral health clinician (BHC) in the neonatal intensive care unit (NICU) setting. It is guided by trauma-informed and culturally informed practices that target the family system and medical system surrounding the infant patient. It discusses the need for perinatal mental health screening and psychosocial assessment in the NICU, and it offers recommendations for the integration of these assessments into standard workflow. The BHC is essential to the provision of interventions that support parental, infant, and staff mental health and well-being in the NICU. Primary targets for BHC intervention and impact may include, but are not limited to, helping parents and staff understand the reality of the traumas faced in the NICU, offering individualized coping resources and group-based support, laying the groundwork for continued processing of the NICU experience, enhancing patient–provider communication, and acting as a liaison for internal and external support resources.
Mental Health and Coping Challenges Among Families in the NICU
Authors: Adams Jennifer Harned , Bromberg Stacey R. , Zimmermann Anna 
Published: August 2022

Abstract
Parents of neonatal intensive care unit (NICU) infants are under a great deal of stress and are at greater risk for perinatal mood and anxiety disorders, including post-traumatic stress disorder, due to factors related to high-risk pregnancy, premature delivery, and maternal and/or infant health factors. Poor emotional adjustment in parents may impact parental health and well-being, engagement in the NICU, outcomes for infants, as well as the larger family unit, including older siblings. Although a number of factors increase the likelihood of emotional distress in NICU families, implementing best practice for communication with families and providing comprehensive education and support can provide a buffering effect.
Role of Behavioral Health Clinicians in Neonatal Follow-Up Settings
Authors: Hoffman Casey , Markovits Annie 
Published: August 2022

Abstract
Neonatal follow-up clinics are a critical resource for high-risk infants and their families after hospital discharge. Behavioral health clinicians (BHCs) are important members of these multidisciplinary teams and serve in many key roles. BHCs bring an expertise in attending to the holistic needs of infants and families. They have specialized training to provide comprehensive assessments for infants and children that includes timely diagnosis, treatment planning, and collaboration with community supports. BHCs partner with families to provide recommendations that prioritize parental goals. BHCs recognize the central role of parent–infant relationships in development and the inextricable link between parent and infant functioning. Therefore, BHCs attend to the wellness of each primary caregiver as well as the adjustment of the entire family unit.