bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polish Adaptation of the TOS Scale in the Adult Population and Comparison with the ORTO-15 Scale
Authors: Gortat Mateusz , Samardakiewicz Marzena 
Published: March 2022

Abstract
BackgroundOrthorexia nervosa (ON) is a concept introduced in 1997. It is defined as excessive concentration on healthy eating. Orthorexia can lead to health loss and disrupt the psychosocial functioning of a person. A significant and current research problem on nervous orthorexia is the lack of appropriate diagnostic tools. One of the promising tools for studying orthorexia behavior is the Teruel Orthorexia Scale (TOS). This scale allows distinguishing pathological orthorexia (Ne) from healthy orthorexia (He), defined as an interest in healthy eating free from psychopathology. There are still no tools to measure orthorexia-related behavior with good psychometric properties. Therefore, this study aimed to adapt and validate Teruel Orthorexia Scale (TOS) in Poland. MethodsThe research involved the evaluation of 680 questionnaires collected in a group of adult Poles (18-68 years old). The research was conducted in the period from June 2019 to March 2020. The diagnostic survey method was used to collect the data. The study used the TOS scale translated into Polish and the Polish adaptation of the ORTO-15 scale. The obtained results were statistically analyzed. The validation procedure included three primary methods: assessment of the discriminant power, reliability, and validity of the tool compared to the ORTO-15 scale. ResultsAlmost all items of the validated scale achieved a satisfactory value of discriminant power >0.3. The individual TOS subscales were characterized by high reliability (TOS He = 0.87; TOS Ne = 0.78). The validity analysis of the validated tool consisting in correlating the obtained results with the results of the ORTO-15 questionnaire showed a significant correlation between the scales (healthy orthorexia r = -0.36; pathological orthorexia r = -0.19). ConclusionsThe Polish version of the TOS scale is a reliable and valuable tool for measuring orthorexia-related behavior in the Polish adult population.
Microbial Extracellular Alkaline Phosphatase Revealed the Linkage Between Carbon and Phosphorus Cycles in the Deepest Ocean
Authors: Fang Jiasong , Cao Junwei , Liu Ying , Deng Junhao , Li Zhenzhen , Luo Min , Yu Xi , Wang Li , Li Jiangyan , Xu Yunping , Wang Jiahua , Li Yongqi , Wang Guangyi , Zhang Li 
Published: March 2022

Abstract
We present the first dataset of microbial extracellular alkaline phosphatase activities (APA) and community compositions measured in the entire water column of the Challenger Deep (CD), Mariana Trench. We show that there existed two distinct P-APA regimes in the CD water column, reflecting different nutrient use strategies of the microbes; that in surface water (50 m) where microorganisms were P-limited (110 nmol L-1) and expressed extremely high APA (average 23.8 nmol h-1) for generating inorganic P, and that in the deep waters (1,000–10,918 m) which were P-replete (average 2.2 mmol L-1), yet microorganisms were carbon-starved and also expressed high levels of APA (average 17.0 nmol h-1). We suggest that microorganisms in the deep waters likely utilized a piggyback strategy to satisfy carbon requirements by expressing elevated APA to decompose dissolved organic phosphorus and concurrently, leading to high concentrations of P in the deep. Our results implicate the intimate connection and coupling between P- and C-cycling mediated by microbes in the hadal biosphere.
Carcinogenic risk in the biliary epithelium of children with congenital biliary dilatation via the DNA damage repair pathway
Authors: Mori Hiroki , Morine Yuji , Yokota Noriko , Ishibashi Hiroki , Shimada Mitsuo 
Published: March 2022

Abstract
Background: Congenital biliary dilatation (CBD) is a high-risk factor for biliary tract cancer (BTC). We have previously reported the potential for carcinogenesis in the biliary epithelium of patients with CBD. In this study, we investigated potential carcinogenetic pathways, focusing on the DNA damage repair response, in children with CBD and compared the findings with adults.Methods: We enrolled six children with CBD and 10 adults with CBD without BTC who underwent extrahepatic bile duct resections, plus four control patients who underwent pancreaticoduodenectomy for non-biliary cancer. Levels of phosphorylated histone H2AX (γH2AX), MRE11, and Ku-70 in the biliary tract epithelium were evaluated by immunohistochemistry.Results: Levels of γH2AX, MRE11, and Ku-70 were significantly higher in the gallbladder epithelium and bile duct epithelium of both children and adults compared with in controls. Conclusions: Children and adults with CBD might develop BTC via the DNA damage repair pathway, as evidenced by increased γH2AX, MRE11, and Ku-70 expression.
Impaired sensitivity to thyroid hormones is associated with elevated blood glucose in coronary heart disease — a RCSCD-TCM study in China
Authors: Yu Lu , Li Zhu , Yang Rongrong , Pan Guangwei , Cheng Qi , He Yuanyuan , Liu Yijia , Liu Fanfan , Ma Mei , Yang Tong , Wang Yang , Su Jinyu , Zheng Yanchao , Gao Shan , Xu Qiang , Li Lin , Yu Chunquan 
Published: March 2022

Abstract
BackgroundThyroid hormone influences glucose homeostasis through central and peripheral regulations. So far, the association between thyroid hormone sensitivity and elevated blood glucose (EBG) in patients with coronary heart disease (CHD) remains unknown. The purpose of this study is to investigate the association between thyroid hormone sensitivity and risk of EBG in patients with CHD, and to further explore their association in different sexes and ages.MethodsThis large multicenter retrospective study included 30,244 CHD patients (aged from 30 to 80) between January 1, 2014 and September 30, 2020. The study calculated the parameters representing central and peripheral sensitivity to thyroid hormones. Central sensitivity to thyroid hormones was assessed by calculating the thyroid feedback quantile-based index (TFQI), thyroid stimulating hormone index (TSHI), and thyrotropin thyroxine resistance index (TT4RI), and peripheral sensitivity to thyroid hormones was evaluated by the ratio of FT3/FT4. Taking normal glucose tolerance (NGT) as a reference, participants with diabetes or prediabetes were assigned to the EBG group. The study used logistic regression to analyze the relationship between central and peripheral thyroid hormone sensitivity and EBG in CHD patients.ResultsAmong the 30,244 participants, 15,493 (51.23%) had EBG. The risk of EBG was negatively associated with TSHI (OR: 0.86; 95%CI: 0.85 to 0.87; P < 0.001), TT4RI (OR: 0.99; 95% CI: 0.99 to 0.99; P<0.001), TFQI (OR: 0.72; 95%CI: 0.70 to 0.73; P <0.001) and PTFQI (OR: 0.60; 95%CI: 0.58 to 0.61; P<0.001). Compared to males and people aged 60 and below, the OR value for EBG was lower in females and those aged over 60 years old. Conversely, EBG risk was positively associated with FT3/FT4 (OR: 1.25; 95% CI: 1.24 to 1.26; P <0.001) and in the sex-categorized subgroups, males had higher OR values than females.ConclusionsThis study shows that the increase in central thyroid hormone sensitivity is associated with the reduced risk of EBG in CHD patients, among which females and people aged over 60 years old are at higher risk.
Balamuthia mandrillaris trophozoites ingest human neuronal cells via a trogocytosis-independent mechanism
Authors: Pengsart Worakamol , Thongkrachang Nongnat , Whangviboonkij Narisara , Sarasombath Patsharaporn Techasintana , Kulkeaw Kasem 
Published: March 2022

Abstract
Background: Environmental protozoa need an adaptation mechanism to survive drastic changes in niches in the human body. In the brain parenchyma, Balamuthia mandrillaris trophozoites, which are causative agents of fatal brain damage, must acquire nutrients through ingestion of the surrounding cells. However, the mechanism the trophozoites deploy for cellular uptake remains unknown.Methods: Amoebic ingestion of human neural cell components was investigated using a coculture of clinically isolated B. mandrillaris trophozoites with human neuroblastoma SH-SY5Y cells. Cell-to-cell interactions were visualized in a three-dimensional manner using a confocal and holotomographic microscope.Results: The B. mandrillaris trophozoites attached themselves to human neuroblastoma SH-SY5Y cells, followed by twisting themselves around the cytoplasmic bridge. Based on fluorescence-based cell tracking, the B. mandrillaris trophozoites inserted invadopodia into the cytoplasm of the human cells. Subsequently, the human protein-enriched components were internalized into the trophozoites in the form of nonmembranous granules, whereas the human lipids were dispersed in the cytoplasm. Intervention of trogocytosis, a process involving nibbling on the parts of target cells, failed to inhibit this cellular uptake.Conclusions: In summary, human cell ingestion by B. mandrillaris trophozoites likely differs from trogocytosis, suggesting that a pathogen-specific strategy can be used to ameliorate brain damage.
Analysis and construction of an exosome derived competing endogenous RNA network for small cell lung cancer in the exoRbase database
Authors: Zhu Kangle , Wang Lian , Chen Zanhao , Ding Jianing , Dong Jixuan , Chen Jinliang 
Published: March 2022

Abstract
A competing endogenous RNA (ceRNA) regulatory network in the blood exosomes of small cell lung cancer (SCLC) patients was constructed by bioinformatics methods to explore the pathogenesis. Blood exosomal gene sequencing data of SCLC patients and normal controls were downloaded from the exorbase 2.0 database, and the expression profiles of exosomal mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) were differentially analyzed using the R language, miRNAs binding to differentially expressed lncRNAs were predicted using the miRcode database, and miRNAs binding to differentially expressed circRNAs were predicted using the Starbase database. The associated mRNAs, circRNAs, lncRNAs, and their corresponding miRNA prediction data were imported into the cytoscape software to visualize the ceRNA network. Enrichment analysis of Go and KEGG was performed and visualized using the R language and Kobas. Cytohubba has used Plug-in identified hub genes. 13 differentially expressed mRNAs, 59 differentially expressed circRNAs, and 40 differentially expressed lncRNAs were filtered out. A ceRNA network was constructed by cytoscape software, and a total of 5 mRNA nodes, 5 lncRNA nodes, 7 circRNA nodes, and 41 miRNA nodes were identified. KEGG enrichment analysis showed that the differentially expressed mRNAs in the regulatory network were mainly enriched on non-small cell lung cancer pathways, and rassf3 was the pathway Among the key genes. Cytohubba to find the most critical hub gene oip5-as.in this study, we successfully constructed a ceRNA regulatory network in the blood exosomes of SCLC patients to provide exact targets for the diagnosis and treatment of SCLC.
miR-124-3p and miR-181a-5p Mediate AT1-Receptor Autoantibody Induced Fetal Rat Cardiac Remodeling via Increased VCAN Expression
Authors: Yue Mingming , Sun Yan , Li Yang , Wang Pengli , He Chunyu , Bai Lina , Wu Ye , Lan Shuhai , Lau Wayne , Ma Xin-Liang , Zhang Suli , Liu Huirong 
Published: March 2022

Abstract
Autoantibodies against angiotensin II type 1 receptor (AT1-AA) are prevalent in preeclampsia. They induce fetal cardiac structural remodeling in late pregnancy via still unknown mechanisms, the subject of this study’s investigation. MicroRNA array revealed differential expression of microRNAs in fetal hearts exposed to AT1-AA. miR-124-3p and miR-181a-5p, the most significantly altered microRNAs, were significantly enriched in the myocardium. Gain- and loss-of-function experiments demonstrated that miR-124-3p and miR-181a-5p governed cardiomyocyte phenotype. Overexpression of miR-124-3p or inhibition of miR-181a-5p activated neonatal rat cardiomyocyte proliferation and size (the converse also being true). Moreover, miR-124-3p overexpression and miR-181a-5p inhibition acted in concert to exacerbate the effects of AT1-AA (increasing the size and proliferation of neonatal rat cardiomyocytes). Through TMT™ quantitative proteomics and luciferase reporter gene assay, miR-181a-5p and miR-124-3p respectively target the 3’ UTR and 5’UTR of VCAN, and oppositely regulate its expression. VCAN knockdown in neonatal rat cardiomyocytes blocked the pathological effect of AT1-AA. Furthermore, ELISA demonstrated positive correlation between AT1-AA expression and VCAN level in preeclamptic patient umbilical cord blood. Our study demonstrates that upregulation of miR-124-3p promotes VCAN expression, and miR-181a-5p downregulation decreases VCAN inhibition, thereby co-mediating AT1-AA induced fetal cardiac remodeling.
Radiographic and clinical outcomes of slope and varus correction anterior closing wedge tibial osteotomy for primary anterior cruciate ligament injuries with steep posterior tibial slope, varus knee deformity, and medial meniscal posterior root tears
Authors: Li Zhong , Liu Juncai , zhou Peng , Deng Xiangtian , Jiang Hao , Li Lingzhi 
Published: March 2022

Abstract
Background: previous biomechanical studies have investigated the influence of coronal and sagittal correction high tibial osteotomy in ACL-reconstructed knees. However, no studies have reported its clinical application. Our objective was to evaluate the radiological and clinical results after simultaneous slope and varus correction anterior closing wedge tibial osteotomy (ACWTO) and anterior cruciate ligament (ACL) reconstruction in patients with ACL deficiency combined with steep posterior tibial slope (PTS), varus deformity, and medial meniscal posterior root tear (MMPRT).Methods: Between January 2018 and December 2021, 5 patients with ACL injuries who had steep PTS, varus deformity, and concomitant MMPRT underwent slope and varus correction ACWTO combined with primary ACLR. The mean follow-up duration was 26.2 months. Preoperative and postoperative radiological assessments included the degree of the femoral tibial angle (FTA), PTS, progression of medial osteoarthritis . For clinical outcomes, we evaluated KT-1000 side-to-side difference, pivot-shift test, International Knee Documentation Committee (IKDC) score, Lysholm score and Tegner activity score preoperatively and at the last follow-up.Results: The mean PTS was 17.0° (range, 16°-19°) preoperatively and 7.8° (range, 6°-9°) postoperatively (P ＜0.01). The mean FTA was 5.6° (range, 5°-7°) preoperatively and -0.6°(range, -2°-0°) postoperatively (P ＜ 0.01). According to the Kellgren-Lawrence classification, 4 patients was grade 1, and 1 patient was grade 2 at the last follow-up. All patients showed statistically significant improvements in knee stability (pre- vs postoperatively：mean side-to-side difference, 9.2 mm vs 0.6mm; and pivot-shift test, 2 grade I and 3 grade II vs 5 grade 0). All patients showed statistically significant improvements in function (pre- vs postoperatively: mean IKDC score, 48.8 vs 89.4; mean Lysholm score, 48.2 vs 93.0; and Tegner activity score, 4.0 vs 6.8). Moreover, no graft rerupture were found at the last follow-up.Conclusion: Slope and varus correction ACWTO combined with primary ACLR is a safe and effective procedure to improve knee stability and function in patients with ACL tear, steep PTS, varus knee deformity, and MMPRT.
Comparison of Nanofiltration+Pervaporation, Pervaporation and Dialysis Processes for Partial Dealcoholization of White Wines
Authors: Calvo José Ignacio , Asensio Jaime , Sainz Daniel , Zapatero Rubén , Carracedo Daniel , Fernández-Fernández Encarnación , Prádanos Pedro , Palacio Laura , Hernández Antonio 
Published: March 2022

Abstract
Membrane dialysis is studied as a promising technique for partial dealcoholization of white wines. The performance of three membrane processes applied for the partial dealcoholization of white wines of the Verdejo variety has been studied in the present work. Combination of Nanofiltration with Pervaporation, single step Pervaporation and, finally, Dialysis, have been applied to White wines from same variety and different vintages. The resulting wines have been chemically and sensorially analyzed and results have been compared with initial characteristics of the wines. From the results obtained, we can conclude that the best procedure consists in the application of Dialysis to the wines which resulted in a reasonable alcohol content reduction while maintaining organoleptic properties and consumer acceptability of the original wine. Therefore, membrane dialysis, as a method of partial dealcoholization of white wines, has undoubted advantages over other techniques based on membranes, which must be confirmed in subsequent studies under more industrial conditions.
Rethinking the Complexity and Uncertainty of Spatial Networks Applied to Forest Ecology
Authors: Wu Hao-Ran , Peng Chen , Chen Ming 
Published: March 2022

Abstract
Characterizing tree spatial patterns and interactions are helpful to reveal underlying processes assembling forest communities. Spatial networks, despite its complexity, are powerful to examine spatial interactions at an individual level using well defined patterns. However, complex tree networks introduce uncertainties. Validation methods are needed in order to assess whether network-based metrics can clearly identify different processes. Here, we constructed three types of tree networks, which reflect various aspects of tree competition. Based on five types of spatial null models and 199 Monte-Carlo simulations, we were able to select network-based metrics that exhibited well performances on distinguishing different processes. This technique was then applied to forest dataset Tuscany of Italy. We found that the average node degree and the cluster coefficient are ideal metrics like the Ripley’s K and paired correlation function. In addition, network approach can identify fine-scale spatial variations of tree competition and its underlying causes. Our analyzes also indicate that a bit of caution is needed when defining the network structure as well as designing network-based metrics. We suggested that validation techniques using corresponding spatial null models are critically important to reduce the negative effects caused by uncertainties of the network.
Effectiveness and safety of inactivated SARS-CoV2 vaccine (BBIBP-CorV) among healthcare workers: A seven-month follow-up study at fifteen hospitals
Authors: Ashmawy Rasha , Kamal Ehab , Sharaf Sandy , Elsaka Noura , Esmail Ola Fahmy , Kabeel Samar , Awd Ahmed , Hassanin Ebtisam , Aboeldahab Heba , Nayle Mai , Afifi Magda , Ibrahim Marwa , Rafaat Raghda , Aly Shahinda , Amin Wagdy 
Published: March 2022

Abstract
OBJECTIVE
Health care workers_ the first line army in the current pandemic_ are the most vulnerable group for COVID-19 infection. Vaccination of health care workers is strongly recommended to salvage our army. However, more research is needed to determine the safest and most effective vaccine. In our study, we assessed the safety and effectiveness of the first approved vaccine in Egypt_ the Sinopharm vaccine.
DESIGN, SETTING:
An ambispective cohort study was conducted in fifteen triage and isolation hospitals, from the 1st of March till the end of September 2021. Participants are either fully vaccinated or unvaccinated.
OUTCOMES AND MEASURES:
Efficacy outcome to assess vaccine effectiveness, severity outcome to assess incidence rate of hospitalized cases (severe to critically ill) or the COVID-19 related mortality, in addition to the safety of the vaccine.
INTERPRETATION:
From the selected hospitals, we included 1228 of 1364 interviewed healthcare workers. Vaccine effectiveness was 67% to protect against symptomatic PCR confirmed cases and 46% for total COVID-19 infection after adjusting hazard ratio. Incidence rate ratios of vaccinated to unvaccinated group were 0.39 (95%CI, 0.24–0.65) for PCR confirmed symptomatic case, 0.63 (95%CI, 0.46–0.86) for total COVID-19 infection, 0.45 (95% CI, 0.15–1.31) for hospitalization, 0.71 (95% CI, 0.07– 6.39) for mechanical ventilation and only one COVID-19 death among unvaccinated group. Most adverse events are mild and well-tolerated. Pregnant and lactating vaccinated mothers did not report any sentinel adverse events.
CONCLUSION
Sinopharm was effective in protecting health care workers from the highly transmissible COVID-19 infection when the predominant Delta variant was ambient.
LncRNA Polymorphism Affects the Prognosis of Gastric Cancer
Authors: Lyu Yanping , Yang Shuangfeng , Lyu Xuejie , Wang Yuan-Liang , Ji Shumi , Kang Shuling , Jiang Yu , Xiang Jianjun , He Chenzhou , Li Peixin , Liu Baoying , Wu Chuancheng 
Published: March 2022

Abstract
Background: Previous studies have found that lncRNA polymorphisms are associated with the prognosis of gastric cancer (GC), but the specific roles of many lncRNA polymorphism sites in gastric cancer are still unclear. The aim of our study is to deeply explore the relationship between genetic polymorphism of lncRNA and the prognosis of GC.Methods: The genotypes of candidate SNPs locus were detected by Sequenom Mass ARRAY SNP. We deeply analyzed the association of lncRNA polymorphisms with GC prognosis by univariate and multivariate COX regression, stratified analysis, conjoint analysis, and log-rank test. Results: We found that mutations at rs2579878 and rs10036719 loci reduced the risk of poor prognosis of GC. Stratified analysis showed that rs2795025, rs10036719 and rs12516079 polymorphisms were all associated with tumor prognosis. In addition, conjoint analyses showed that the interaction between these two polymorphic sites, rs2795025 and rs12516079, could increase the risk of poor prognosis. Multivariate analysis also found that AG/AA genotype of rs10036719 and AG genotype of rs12516079 were independent prognostic factors. Moreover, we also found that the high express of both CCDC26 and LINC02122 were shown to be associated with the poor survival status of GC patients.Conclusions: We found that the genetic polymorphism of lncRNA plays a role in the development of GC and is closely related to the survival time of patients. Our results could serve as a predictor to the prognosis of GC.
Characterization and Evaluation of Cold Atmospheric Plasma as Seedborne Fungal Disinfectant and Promoting Mediator for Physico-Chemical Characteristics of Moringa Oleifera Seedlings
Authors: Ouf Salama A. , Almarashi Jamal Q. M. , Mohamed Abdel-Aleam H. 
Published: March 2022

Abstract
Large volume atmospheric pressure glow discharge plasma was developed and investigated to enhance the biological activities of M. oleifera seeds. The generated plasma was characterized optically, electrically, and spectroscopically. The large volume plasma size can reach 40 mm gape and 150 mm diameter. One hundred ns magnetic pulse compression high voltage system was used to generate the plasma where the current increases with increasing applied voltage at a constant frequency and discharge gap while the current decreases with increasing discharge gap at constant voltage and frequency. The plasma homogeneity was enhanced with higher applied voltage and frequency. Nitrogen second positive system bands dominated the plasma emission spectra, and its intensity increased with increasing applied voltage and frequency. There was a steady reduction in the total count of emerged seedborne fungi on the application of cold plasma with complete elimination of fungi at ≥ 13 A. The low doses of cold plasma (3.9 and 5.8 A) induced an increase in the seed germination, a significant increase in chlorophyll content (chl a and chl b) and antioxidant activities of the seedlings emerged from soaked or wet seeds rather than dry seeds. There was a steady reduction in the total count of emerged fungi reaching complete decontamination of seedborne fungi at higher plasma doses (≥ 13 A). The plasma was harmful when applied at higher doses (≥ 13 A) and more harmful to the wet seeds compared to the dry ones, where the chlorophyll a content of the seedling developed from 1% H2O2 soaked seeds.