bluuebunny/crossref_metadata_2025_split huggingface dataset:
PKM2 allosteric converter: a self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy
Authors: Wang Lu , Fu Bo , Hou Da-Yong , Lv Yulin , Yang Guang , Li Cong , Shen Jiachen , Kong Bin , Zheng Libo , Qiu Yu , Wang Honglei , Liu Chen , Zhang Jianji , Li Li-Li , Wang Hao , Xu Wanhai 
Published: May 2022

Abstract
Stronger intrinsic Warburg effect and resistance to chemotherapy are the responses to high mortality of renal cell carcinoma (RCC). Pyruvate kinase M2 (PKM2) plays an important role in this process. Promoting PKM2 conversion from dimer to tetramer is a critical strategy to inhibit Warburg effect and reverse chemotherapy resistance. Herein, a PKM2 allosteric converter (PAC) is constructed based on the “in vivo self-assembly” strategy, which is able to continuously stimulate PKM2 tetramerization. The PAC contains three motifs, a serine site that is protected by enzyme cleavable β-N-acetylglucosamine, a self-assembly peptide and a aggregation-induced emission (AIE) motif. Once PAC nanoparticles reach tumor site via the enhanced permeability and retention (EPR) effect, the protective and hydrophilic β-N-acetylglucosamine will be removed by over-expressed O-GlcNAcase (OGA), causing self-assembled peptides to transform into nanofibers with large serine (PKM2 tetramer activator) exposure and long-term retention, which promotes PKM2 tetramerization continuously. Our results show that PAC-induced PKM2 tetramerization inhibits aberrant metabolism mediated by Warburg effect in cytoplasm. In this way, tumor proliferation and metastasis behavior could be effectively inhibited. Meanwhile, PAC induced PKM2 tetramerization impedes the nuclear translocation of PKM2 dimer, which restores the sensitivity of cancer cells to first-line anticancer drugs. Collectively, the innovative PAC effectively promotes PKM2 conversion from dimer to tetramer, and it might provide a novel approach for suppressing RCC and enhancing chemotherapy sensitivity.
Targeting STAT5b regulated signaling suppresses neuroinflammation and protects neuron in ischemic stroke
Authors: Pu Zhijun , Xia Shengnan , Shao Pengfei , Bao Xinyu , Wu Dan , Xu Yun 
Published: May 2022

Abstract
Inflammatory storm after ischemia-reperfusion contributes significantly to prognosis, and STAT5 is proving to be a highly effective anti-inflammatory therapy with great potential. The inhibition of STAT5 has demonstrated significant anti-inflammation and therapeutic effects, but studies on neuroinflammation and brain injury from ischemia-reperfusion are rare. It is the first time to define the dauricine as a STAT5 inhibitor, and found that dauricine mainly bind with STAT5b at Tyr-699 instead of STAT5a. Dauricine suppressed the inflammation cytokines Eotaxin, KC, TNF-α, IL-1α, IL-1β, IL-6, IL-12β, IL-17α, and also inhibited the NF-kappaB activation. These results suggest that dauricine might protect the brain from the injury of post-ischemia-reperfusion via mediating the NF-kappaB activation by inhibiting the STAT5b. As a potential therapeutic target for neuroinflammation, STAT5b needs to be raised concern in ischemic stroke.
Formation and stability of bulk nanobubbles generated by gas–liquid mixing
Authors: Lee Jeong Il , Han Jung-Geun , Kim Jong-Min 
Published: May 2022

Abstract
As the field of application of nanobubbles (NBs) expands, more in-depth research into NB generation techniques is required. In this study, the NB characteristics generated by gas–liquid mixing and an NB generation mechanism were investigated according to the different liquid volume fraction (LVF). In the gas–liquid mixing method, we confirmed that the important factors for NB generation are the shear force applied to the trapped bubbles and the amount of trapped bubbles in the liquid. Based on these factors, NBs were generated at more than 10×108 NBs/ml and remained in a stable state for seven days. This study proposes a more specific NB generation mechanism and a valuable methodology for high-concentration NB generation through the gas–liquid mixing method.
The Women’s Health Initiative cancer survivorship clinic incorporating electronic patient reported outcomes: a study protocol for the Linking You to Support and Advice (LYSA) randomized controlled trial.
Authors: Kearns Noreen , Raigal-Aran Laia , O'Connell Kate , Davis Andrea , Bermingham Katie , O'Reilly Seamus , Collins Dearbhaile C , Corrigan Mark , Coulter John , Cleary Vicki , Cushen Samantha , Flavin Aileen , Byrne Fiona , O'Grady Aisling , O'Neill Deirdre , Murphy Aileen , Dahly Darren , Palmer Brendan , Connolly Roisin M , Hegarty Josephine 
Published: May 2022

Abstract
BackgroundThe improved survival rate for many cancers in high income countries demands a coordinated multidisciplinary approach to survivorship care and service provision to ensure optimal patient outcomes and quality of life. The primary aim of this randomized controlled trial (RCT) is to assess the feasibility of introducing a Women’s Health Initiative Cancer Survivorship Clinic in Ireland. MethodsThe trial comprises an intervention and control arm. Two hundred participants will be recruited. Key eligibility 1) women with early-stage hormone receptor-positive breast or gynaecologic cancer (cervix or endometrial), within 12 months of completion of primary curative therapy, and 2) access to the internet. The complex intervention comprises a nurse-led pilot clinic targeting symptom management through a trigger alert system, utilizing electronic patient reported outcome (ePRO) assessments at baseline, and 2, 4, 6, 8, 10, and 12 months. It also includes dietitian-led component monitoring diet and nutritional status. The control group will receive their usual care pathway standard of care and attend the cancer survivorship clinic and complete ePRO assessments at the start and end of the study. Primary endpoint (feasibility) includes: the proportion of enrolled participants who complete baseline and follow-up ePRO surveys, and partake in health professional consultations after ePRO data triggers. Secondary endpoints include: changes in cancer-related symptom scores assessed by ePROs, health related-Quality of Life Questionnaire (QLQ) scores, Appraisal Self-Care Agency-R scores, and adjuvant endocrine therapy medication adherence. A process evaluation will capture the experiences of participation in the study, and the healthcare costs will be examined as part of the economic analysis.Ethical approval was granted in December 2020, with accrual commencing in March 2021.DiscussionThis protocol describes the implementation of a parallel arm RCT which examines the feasibility of delivering a Cancer Survivorship Clinic. The ePRO is an innovative symptom monitoring system which detects treatment related affects and provides individualized support for cancer survivors. The findings will provide direction for the implementation of future survivorship care. Trial Registration ClinicalTrials.gov, NCT05035173. Registered September 5th 2021 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT05035173?cntry=IE&city=Cork&draw=1&rank=10
A Spanish cross-cultural adaptation of the Quality in Psychiatric Care – Forensic In-patient Staff (QPC-FIPS) instrument: Psychometric properties
Authors: Campo Marta Domínguez-del , Moreno-Poyato Antonio R. , Puig-LLobet Montserrat , Lluch-Canut María Teresa , Rodríguez-Zunino Natalia F. , Tomás-Jiménez Manuel , Sanchez-Balcells Sara , Schröder Agneta , Lundqvist Lars-Olov , Escuder-Romeva Gemma , Roldán-Merino Juan F. 
Published: May 2022

Abstract
"Quality in Psychiatric Care - Forensic Inpatient Staff (QPC-FIPS) is an instrument of Swedish origin validated to measure the perception of the quality of mental health care provided by forensic psychiatry professionals. The aim of this study was to cross-culturally adapt the QPC-FIPS instrument and to evaluate the psychometric properties of the Spanish version of the instrument. A psychometric study was carried out. For validity, content validity, convergent validity and construct validity were included. For reliability, the analysis of internal consistency and temporal stability was included. The sample consisted of 153 mental health professionals from four Forensic Psychiatry units. The adapted Spanish version of the QPC-FIPS scale was configured with the same number of items and dimensions as the original. The psychometric properties, in terms of temporal stability and internal consistency, were adequate and the factor structure, such as the homogeneity of the dimensions of the Spanish version of the QPC-FIPS, was equivalent to the original Swedish version. We found that the QPC_FIPS-Spanish is a useful, valid and easy-to-apply instrument for assessing the self-perception of professionals regarding the care they provide, as well as, identifying elements for the improvement of the quality of care.
Comprehensive analysis reveals independent prognostic biomarkers in glioblastoma
Authors: Jin Xingbing , Zhang Lily , Deng Bin 
Published: May 2022

Abstract
Background
Glioblastoma (GBM) is a highly malignant intracranial tumor with an extremely poor prognosis. Screening and identification of potential prognostic biomarkers are essential for clinical treatment of GBM.
Methods
In this study, a total of 199 differentially expressed genes (DEGs) were identified based on GSE4290, GSE19728 and GSE50161 datasets. Function enrichment analyses and protein-protein interaction (PPI) network were performed. As a result, 10 genes (CDK1, TOP2A, RRM2, DTL, PBK, NUSAP1, NDC80, DLGAP5, KIF14, FOXM1) were selected as hub genes using CytoHubba in Cytoscape. Subsequently, GEPIA, Human Protein Atlas, PROGgeneV2 and cBioPortal databases were used to study the roles of hub genes in the prognosis of GBM.
Results
The results showed that 10 hub genes were over-expression in GBM patients and higher expressions of hub genes were significantly correlated with shorter overall survival (OS) in GBM patients. Multivariate analysis indicated that RRM2, DLGAP5 and KIF14 were independent prognostic factors of GBM patients. Three independent prognostic biomarkers were also found to be correlated with immune cell infiltration and clinical features of glioma patients.
Conclusion
Our results indicated that RRM2, DLGAP5 and KIF14 could be independent prognostic biomarkers of GBM patients.
Repeated psychological stress, chronic vicarious social defeat stress, evokes irritable bowel syndrome-like symptoms in mice
Authors: Yoshioka Toshinori , Ohashi Misaki , Matsumoto Kenjiro , Omata Tomoki , Hamano Takumi , Yamazaki Mayuna , Okano Kotaro , Kobayashi Riho , Yamada Daisuke , Hada Noriyasu , Kato Shinichi , Saitoh Akiyoshi 
Published: May 2022

Abstract
Increasing evidence has demonstrated that emotional states and intestinal conditions are inter-connected in so-called “brain–gut interactions.” Indeed, many psychiatric disorders are accompanied by gastrointestinal symptoms, such as the irritable bowel syndrome (IBS). However, the functional connection remains elusive, partly because there are few useful experimental animal models. Here, we focused on a highly validated animal model of stress-induced psychiatric disorders, such as depression, known as the chronic vicarious social defeat stress (cVSDS) model mice, which we prepared using exposure to repeated psychological stress, thereafter examining their intestinal conditions. In the charcoal meal test and the capsaicin-induced hyperalgesia test, cVSDS model mice showed a significantly higher intestinal transit ratio and increased visceral pain-related behaviors, respectively. These changes persisted over one month after the stress session. On the other hand, the pathological evaluations of the histological and inflammatory scores of naive and cVSDS model mice did not differ. Furthermore, keishikashakuyakuto—a kampo medicine clinically used for the treatment of IBS—normalized the intestinal motility change in cVSDS model mice. Our results indicate that cVSDS model mice present IBS-like symptoms such as chronic intestinal peristaltic changes and abdominal hyperalgesia without organic lesion. We therefore propose the cVSDS paradigm as a novel animal model of IBS with wide validity, elucidating the correlation between depressive states and intestinal abnormalities.
LXRB-SREBP1 axis regulating lipogenic homeostasis by controlling the synthesis of polyunsaturated fatty acids in goat mammary epithelial cells
Authors: Zhang Wenying , Zhang Changhui , Luo Jun , Xu Huifen , Liu Jianxin , Loor Juan J. , Shi Hengbo 
Published: May 2022

Abstract
Background: Liver X receptor beta (LXRB) is the predominant LXR subtype in ruminant mammary cells. Whether and how LXRB plays a role in the synthesis of polyunsaturated fatty acids (PUFA) in the mammary gland remains to be determined. In the current study, we used overexpression and knockdown of LXRB in goat primary mammary epithelial cells (GMEC) to evaluate abundance of lipogenic enzymes, fatty acid profiles, content of lipid stores and activity of the stearoyl-CoA desaturase (SCD1) promoter. Results: Overexpression of LXRB markedly upregulated the protein abundance of LXRB while incubation with siRNA targeting LXRB markedly decreased abundance of LXRB protein. Overexpression of LXRB plus ligand T0901317 (T09) dramatically upregulated genes encoding proteins related to PUFA synthesis. Compared with the control, cells overexpressing LXRB plus T09 had greater concentrations of C16:0, 16:1, 18:1n7,18:1n9 and C18:2 as well as desaturation and elongation indices of C16:0. Furthermore, LXRB overexpressing cells incubated with T09 had greater levels of triacylglycerol and cholesterol. Knockdown of LXRB in cells incubated with T09 led to downregulation of genes encoding elongases and desaturases. In contrast, knockdown of LXRB attenuated the increase in TAG and cholesterol that was induced by T09. In cells treated with DMSO, knockdown of LXRB increased the concentration of C16:0 at the expense of C18:0, while a significant decrease in C18:2 was observed in cells incubated with both siLXRB and T09. The abundance of sterol regulatory element binding transcription factor 1 (pSREBP1) and its mature fragment (nSREBP1) was upregulated by T09, but not LXRB overexpression. In the cells cultured with T09, knockdown of LXRB downregulated the abundance for pSREBP and nSREBP1. Luciferase reporter assays with site directed mutagenesis of SREBP1 response elements (SRE) revealed that that the SCD1 promoter with the wild type or SRE mutation decreased markedly when LXRB was knocked down. Activity of the SCD1 promoter that was induced by T09 was impaired when the SRE mutation was introduced. Conclusion: There is an important role of LXRB-SREBP1 axis in the synthesis of PUFA via the regulation of genes encoding elongases and desaturases. Thus, this network elicits broad effects on lipid homeostasis in ruminant mammary gland.
Fracture dislocation of the cervical spine and common carotid artery medialization
Authors: FERRACCI François-Xavier , HEUZE Donatien , SACCO Riccardo , CURADO Jonathan , MONNOT Antoine , DUPARC Fabrice , OULD-SLIMANE Mourad 
Published: May 2022

Abstract
A 68-year-old female presented acute neck pain and incomplete tetraplegia following a fall on the stairs. Radiographs, contrast enhanced computed tomography scan and magnetic resonance of the cervical spine revealed a C5-C6 bi-articular dislocation. A detailed preoperative assessment of the images discovered a medialization of the left common carotid artery. An external reduction and a left anterior cervical approach allowed a careful management of the vascular malformation and an anterior C5C6 arthrodesis. At six months, a full neurological recovery was assessed and radiographs demonstrated successful fusion of the cervical arthrodesis.Anatomical features such as medialization of the common carotid artery may affect patients with traumatic cervical spine injuries. The severity of the traumatic bone lesions should not overshadow the preoperative analysis of the adjacent anatomical structures encountered during the surgical approach, even in an emergency situation.
Preparation and Properties of Lignin-Based Carbon/ZnO Photocatalytic Materials
Authors: Li Tianyang , Pan Hong , Xu Lihui , Shen Yong , Li Keting 
Published: May 2022

Abstract
Lignin-based carbon/ZnO (LCZ) composites were prepared by one-step carbonization method using lignin as carbon source. The effects of different carbonization conditoions (temperature, carbonization time and QAL addition) on the pore structure of the composites were investigated. The results showed that the obtained LCZ composite had the largest specific surface area (317.765 m2·g− 1) and pore volume (0.4344 mL·g− 1) when the QAL addition was 4 g and carbonization at 600°C for 2 h. Due to the tight combinationg of carbon and ZnO, the photodegradation performance of the prepared LCZ under simulated solar light (500 W Xe lamp) irradiation was improved for the rapid transfer of photogenerated electrons. Compared with pure ZnO, the degradation rate of LCZ reached 92% and 91% of methyl orange and rhodamine B in 2 hours. Meanwhile, LCZ had good acid-base stability and cyclic stability.
MTMR14 is a potential independent prognostic factor in glioma patients and induces glioma cell apoptosis through the Akt/mTOR pathway
Authors: liu ruen , wang changfeng , wang zhiwen , lv qiaoli , xiang hui , ou yue , chen xianjun , liu jiahao , zheng chenpeng 
Published: May 2022

Abstract
Purpose: Novel phosphoinositide phosphatase MTMR14 is responsible for regulating inflammatory metabolism, stable aging, and autophagy. This study investigated the clinicopathological significance of MTMR14 expression in glioma.Methods: mRNA expression profiles and clinical information of 703 glioma patients were downloaded from The Cancer Genome Atlas database. Wilcoxon signed-rank test and logistic regression were used to evaluate the relationship between clinical factors and MTMR14 expression. Cox regression and the Kaplan–Meier method were used to determine the clinical factors associated with overall survival of patients with glioma. Using the STRING database and the TIMER2.0 database, proteins and tumor immune infiltrating cells interacting with MTMR14 were identified. MTMR14 of U87 and U251 cells was knocked out by transfection. Interference efficiency was determined by a real-time fluorescence quantitative nucleic acid amplification test; the CCK-8 experimental wound healing test was performed, and a flow cytometry apoptosis assay was used to observe the effect of MTMR14 knockout on glioma cells. Results:MTMR14 expression levels were highly correlated with grade, age, and survival time of glioma patients. The expression level of MTMR14 was correlated with overall survival.The proliferation and migration of U87 and U251 cells were weakened and apoptosis was promoted after knocking MTMR14.Conclusions: MTMR14 was a potential independent prognostic factor for glioma patients, participating in the regulation of various signaling pathways and influencing tumor immune microenvironment. In cell experiments, deletion of MTMR14 resulted in decreased proliferation and migration ability of glioma cells, and activation of the Akt/ mTOR pathway led to glioma cell apoptosis.
Construction of a minicellulosome-producing consolidated bioprocessing reaction system for simultaneous saccharification and co-fermentation from Pennisetum purpereum
Authors: Liu Han , Du Jiliang , Wang Xuxin , Kang Zhuoran , Hou Chenglin , Yang Xiushan , Tian Shen 
Published: May 2022

Abstract
The enhanced hydrolysis of xylan-type hemicellulose is important to maximize ethanol production yield and substrate utilization rate in lignocellulose-based simultaneous saccharification and co-fermentation system. In this study, we conduct d-integration CRISPR Cas9 to achieve multicopy chromosomal integration with high efficiency of reductase-xylitol dehydrogenase pathway in Saccharomyces cerevisiae. Subsequently, a mincellulosome attaching with hemicellulose-degrading enzymes on this xylose-utilizing recombinant yeast strain is developed for synergistic catalysis and co-fermentation from steam-exploded Pennisetum purpereum. Despite the accumulation of xylitol, the maximum ethanol titer of genetic engineered yeast strain reached 4.77 g/l with the cellulose conversion of 97.02% and hemicelluose conversion of 67.45% under 30 ºC after 96 h with the addition of commercial cellulase. The elaborated cellulosomal organization toward genetic engineering of an industrially important microorganism presents a designed approach for advanced lignocellulolytic potential and improved capability of biofuel processing.
Attention Vision Transformers for Human Fall Detection
Authors: Bakshi Satyake 
Published: May 2022

Abstract
In recent years, attention transformers have proven to be instrumental in natural language processing (NLP)-based tasks such as sentence classification and language translation. However, their application has been recently extended to large-scale object recognition tasks. In this work, Vision Transformer with attention has been investigated for the detection of human falls and ADLs (Activities of Daily Living) from time series-based signals. The Vision Transformer model has been trained and validated using the acceleration signals of waist-worn Inertial Measurement Unit (IMU) sensors obtained from the IMU Falls dataset[1]. The model is also trained and validated on the popular SiSFall dataset[2]. The model is also investigated by independently training three different cases of patch size and attention heads. It was observed that a larger patch size resulted in significant performance deterioration. Additionally, a smaller patch size took longer to train and was computationally expensive. The model performed (best case) with an Accuracy (%) of 99.9 ± 0.1 and a True Positive Rate (%) of 99.9 ± 0.1 on the SFU-IMU dataset and with an Accuracy (%) of 99.8 ± 0.25 and a true positive rate (%) of 99.87 ± 0.3 on the SISFALL dataset. Overall, the results show that Transformers are highly robust in the detection of human falls and nonfalls/ADLs, subject to the appropriate patch size.