bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Antibacterial and Antifungal Activities of a Medicinal Soap Made from Zingiber officinale and Syzygium aromaticum Essential Oils
Authors: Mbouche Jodie Valdese , Ngamga Fabrice Hervé Njike , Ngangoum Eric Serge , Djikeng Fabrice Tonfack 
Published: September 2022

Aims: The objective of this study is to evaluate the antibacterial and sensory properties of a formulated medicinal soap.
Study Design: Extraction of essential oils, purchase of vegetable oils and caustic soda, soap formulation, testing of its antibacterial and antifungal activities, evaluation of its acceptability.
Place and Duration of Study: Research unit of biochemistry of medicinal plant, food and nutritional sciences, Department of Biochemistry, Faculty of Science, University of Dschang from October 2019 to July 2020.
Methodology: Cold saponification method was used for the production of soap using the following ingredients: Palm kernel oil, olive oil, coconut oil, Palm oleic, caustic soda, essential oils from ginger roots and cloves buds. The formulated soap was used for the evaluation of its anti-bacterial and anti-fungal activities as well as its acceptability using the 9-points hedonic scale. The soap was tested on the following microorganisms: Pseudomonas aeruginosa PA01, Staphylococcus aureus 56, Staphylococcus aureus 18, Staphylococcus aureus ATCC 25923, Candida albican, Candida tropicalis.
Results: Results showed that the produced soap has bactericidal activities against staphylococcus aureus 18 and staphylococcus aureus ATCC 25923 and a bacteriostatic activity against staphylococcus aureus 56. Its activity against fungi was very low. The results of the sensory analysis of this product showed that it can be accepted by consumer since the lowest score was 6.55 in a scale of 9.
Conclusion: Results showed that the produced soap has bactericidal activities against staphylococcus aureus 18 and staphylococcus aureus ATCC 25923 and a bacteriostatic activity against staphylococcus aureus 56. It was accepted with a score of at least 6.55/9 by the participants.
A Water‐Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron‐Protective Effect on Cerebral Ischemia by Mitophagy Activation
Authors: Cen Juan , Zhang Runfang , Zhao Tingkui , Zhang Xin , Zhang Chuan , Cui Jie , Zhao Keqing , Duan Shaofeng , Guo Yuqi 
Published: September 2022

AbstractThe existing treatments for ischemic stroke cannot meet the clinical needs so far. Quercetin (QT) is an effective apoptosis inhibitor and antioxidant flavonoid, but its water solubility is poor and has no targeting. In this study, QT is modified with hyaluronic acid (HA) to form a water‐soluble conjugate HA‐QT, which can specifically bind to CD44 receptors and response to hyaluronidase. Next, a novel delivery system SS31‐HA‐QT is prepared by further modification with SS31, a polypeptide capable of penetrating the blood‐brain barrier (BBB) and indiscriminately targeting mitochondria. Meanwhile, IR780, a near‐infrared dye, is conjugated onto HA‐QT and SS31‐HA‐QT to form diagnosis tools to trace HA‐QT and SS31‐HA‐QT. In vitro and in vivo results shows that SS31 can four‐fold increase the drug penetration into BBB without any toxicity. The highly expressed CD44 and hyaluronidase in ischemic area ensured the targeted delivery of QT to the ischemic region. Importantly, the mitochondrial targeting of damaged neurons is also achieved by SS31. Further studies confirmed that SS31‐HA‐QT exerted neuron‐protection by activating mitophagy, and its mechanism involved Akt/mTOR related TFEB and HIF‐1αactivation. Hence, SS31‐HA‐QT shall be a promising neuroprotective drug due to its high water‐solubility, superior triple‐targeted neuroprotective ability, low toxicity, and high efficiency.
Kilogram‐Scale Fabricated Organic Long‐Persistent Luminescence Materials with Multi‐Level Temperature Response
Authors: Liu Man , Zong Jun , Wang Lichang , Liu Dongzhi , Wang Tianyang , Hu Wenping 
Published: September 2022

AbstractPurely organic long‐persistent luminescence materials (OLPLMs) have shown great application potential in the fields of anti‐counterfeiting and information encryption, due to their eco‐friendly preparation, low synthesis cost, and easy‐to‐tune luminescence. Notably, the development of efficient large‐scale fabrication methods will facilitate the commercial application of OLPLMs. Here, we realized the kilogram‐scale preparation of organic doped‐crystals with different long‐persistent luminescence durations by a facile water‐based method under ambient conditions. Additionally, the function of reversible multi‐level temperature response of LPL performance based on this kind of OLPLMs has been achieved, showing the unique application prospects in the field of anti‐counterfeiting and information encryption. This work provides an effective strategy for large‐scale fabrication of OLPLMs, and will accelerate the research and development of temperature‐responsive OLPLMs.
Thermal Runaway of Nonflammable Localized High‐Concentration Electrolytes for Practical LiNi0.8Mn0.1Co0.1O2|Graphite‐SiO Pouch Cells
Authors: Wu Yu , Feng Xuning , Yang Min , Zhao Chen‐Zi , Liu Xiang , Ren Dongsheng , Ma Zhuang , Lu Languang , Wang Li , Xu Gui‐Liang , He Xiangming , Amine Khalil , Ouyang Minggao 
Published: September 2022

AbstractWith continuous improvement of batteries in energy density, enhancing their safety is becoming increasingly urgent. Herein, practical high energy density LiNi0.8Mn0.1Co0.1O2|graphite‐SiO pouch cell with nonflammable localized high concentration electrolyte (LHCE) is proposed that presents unique self‐discharge characteristic before thermal runaway (TR), thus effectively reducing safety hazards. Compared with the reference electrolyte, pouch cell with nonflammable LHCE can increase self‐generated heat temperature by 4.4 °C, increase TR triggering temperature by 47.3 °C, decrease the TR highest temperature by 71.8 °C, and extend the time from self‐generated heat to triggering TR by ≈8 h. In addition, the cell with nonflammable LHCE presents superior high voltage cycle stability, attributed to the formation of robust inorganic‐rich electrode–electrolyte interphase. The strategy represents a pivotal step forward for practical high energy and high safety batteries.
Visible Light‐Promoted Fluorescein/Ni‐Catalyzed Synthesis of Bis‐(β‐Dicarbonyls) using Olefins as a Methylene Bridge Synthon
Authors: Palani Pushbaraj , Arumugam Ajithkumar , Anandan Mageshwari , Nutalapati Venkatramaiah , Govindan Karthick , Lin Wei‐Yu , Senadi Gopal Chandru 
Published: September 2022

AbstractHerein, we report the synthesis of methylene‐bridged bis‐1,3‐dicarbonyls from olefins and 1,3‐dicarbonyls using a photoredox/nickel‐catalyzed synergistic approach. Styrene is reported as methylene one‐carbon (C1) synthons in the synthesis of bis‐(β‐dicarbonyls) for the first time. The deuterated study showed the incorporation of styrene‐d3 in the product by NMR and Mass analysis. Fluorescence quenching and cyclic voltammetry (CV) suggest that the reaction proceeds via an energy transfer process (EnT). The key aspects of the protocol are the use of benign photocatalyst fluorescein, peroxide‐free, air as oxidant, broader substrate scope, and 56% to 99% product yields. The synthetic application of the product has been extended to construct tetrasubstituted pyridines in a sequential one‐pot manner, bis‐isoxazole, and bis‐pyrazole.
[3+3] Annulation Reaction of 2‐Amino‐4H‐chromen‐4‐ones with Cinnamaldehydes or Aromatic Aldehydes and Ethanal in One Pot: Access to 2‐Aryl‐5H‐Chromeno[2,3‐b]pyridin‐5‐ones
Authors: Gan Jianbo , Xin Junhu , Xue Yuhang , Wang Cunde 
Published: September 2022

AbstractA formal [3+3] cascade annulation strategy for the synthesis of 2‐arylchromeno[2,3‐b]pyridinones has been developed using 2‐aminochromones and substituted cinnamaldehydes or aromatic aldehydes and ethanal as the substrates. The strategy supplies a novel and atom‐economical method of accessing a broad range of chromeno[2,3‐b]pyridine derivatives in good yields with good functional‐group tolerance. The method highlights the inherent practicality of this synthetic transformation.
Linker Redox Mediated Control of Morphology and Properties in Semiconducting Iron‐Semiquinoid Coordination Polymers**
Authors: Wang Lei , Papoular Robert J. , Horwitz Noah E. , Xie Jiaze , Sarkar Arup , Campisi Dario , Zhao Norman , Cheng Baorui , Grocke Garrett L. , Ma Tengzhou , Filatov Alexander S. , Gagliardi Laura , Anderson John S. 
Published: September 2022

AbstractThe emergence of conductive 2D and less commonly 3D coordination polymers (CPs) and metal–organic frameworks (MOFs) promises novel applications in many fields. However, the synthetic parameters for these electronically complex materials are not thoroughly understood. Here we report a new 3D semiconducting CP Fe5(C6O6)3, which is a fusion of 2D Fe‐semiquinoid materials and 3D cubic Fex(C6O6)y materials, by using a different initial redox‐state of the C6O6 linker. The material displays high electrical conductivity (0.02 S cm−1), broad electronic transitions, promising thermoelectric behavior (S2σ=7.0×10−9 W m−1 K−2), and strong antiferromagnetic interactions at room temperature. This material illustrates how controlling the oxidation states of redox‐active components in conducting CPs/MOFs can be a “pre‐synthetic” strategy to carefully tune material topologies and properties in contrast to more commonly encountered post‐synthetic modifications.
Double‐layer Composite Gel Polymer Electrolyte for Organic Sodium‐Metal Batteries
Authors: Wang Shaolong , Ding Chaojian , Tian Hao , Huang Weiwei , Zhang Qichun 
Published: September 2022

AbstractOrganic cathode materials have the advantages of abundant raw materials, high theoretical specific capacity, controllable structure and easy recycling. Pyrene‐4,5,9,10‐tetraone (PTO), as one of the typical organic cathode materials, achieves efficient storage and release of Na+. However, its good solubility in traditional organic liquid electrolytes is detrimental to the cyclic stability of batteries. To address this issue, the double‐layer composite gel polymer electrolyte (DLCGPE) consisting of poly (ionic liquid) gel polymer electrolyte and plastic crystal electrolyte was developed and applied to organic sodium‐metal batteries. This as‐prepared DLCGPE displays an ionic conductivity of 2.17×10−4 S cm−1 and an electrochemical window of 4.8 V. The as‐fabricated sodium‐symmetric batteries maintain interfacial stability after 500 h of cycling. Furthermore, the PTO/Na batteries could also retain a specific capacity of 201 mAh g−1 after 300 cycles, confirming that DLCGPE achieves the purpose of inhibiting PTO dissolution and maintaining batteries stability. This work broadens the application of asymmetric electrolytes in organic secondary battery.
Separate scoring algorithms for specific identification priorities optimize the screening properties of the Screening Tool for Autism in Toddlers (STAT)
Authors: Attar Shana M. , Ibanez Lisa V. , Stone Wendy L. 
Published: September 2022

AbstractThe Screening Tool for Autism in Toddlers (STAT) is a validated stage‐2 autism spectrum disorder (ASD) screening measure that takes 20 minutes to administer and comprises 12 play‐based items that are scored according to specific criteria. This study examines an expanded version (STAT‐E) that includes the examiner's subjective ratings of children's social engagement (SE) and atypical behaviors (AB) in the scoring algorithm. The sample comprised 238 children who were 24–35 months old. The STAT‐E assessors had limited ASD experience to mimic its use by community‐based non‐specialists, and were trained using a scalable web‐based platform. A diagnostic evaluation was completed by clinical experts who were blind to the STAT‐E results. Logistic regression, ROC curves, and classification matrices and metrics were used to determine the screening properties of STAT‐E when scored using the original STAT scoring algorithm versus a new algorithm that included the SE and AB ratings. Inclusion of the SE and AB ratings improved positive risk classification appreciably, while the specificity declined. These results suggest that the STAT‐E using the original STAT scoring algorithm optimizes specificity, while the STAT‐E scoring algorithm with the two new ratings optimizes the positive risk classification. Using multiple scoring algorithms on the STAT may provide improved screening accuracy for diverse contexts, and a scalable web‐based tutorial may be a pathway for increasing the number of community providers who can administer the STAT and contribute toward increased rates of autism screening.
De novo assembly and annotation of the CHOZN® GS−/− genome supports high‐throughput genome‐scale screening
Authors: Kretzmer Corey , Narasimhan Rajagopalan Lakshmi , Lal Rahul Deva , Balassi Vincent , Ravellette James , Kotekar Manjunath Ajaya Kumar , Koshy Jesvin Joy , Viano Marta , Torre Serena , Zanda Valeria M. , Kumravat Mausam , Saldanha Keith Metelo Raul , Chandranpillai Harikrishnan , Nihad Ifra , Zhong Fei , Sun Yi , Gustin Jason , Borgschulte Trissa , Liu Jiajian , Razafsky David 
Published: September 2022

AbstractChinese hamster ovary (CHO) cells have been used as the industry standard for the production of therapeutic monoclonal antibodies for several decades. Despite significant improvements in commercial‐scale production processes and media, the CHO cell has remained largely unchanged. Due to the cost and complexity of whole‐genome sequencing and gene‐editing it has been difficult to obtain the tools necessary to improve the CHO cell line. With the advent of next‐generation sequencing and the discovery of the CRISPR/Cas9 system it has become more cost effective to sequence and manipulate the CHO genome. Here, we provide a comprehensive de novo assembly and annotation of the CHO‐K1 based CHOZN® GS−/− genome. Using this platform, we designed, built, and confirmed the functionality of a whole genome CRISPR guide RNA library that will allow the bioprocessing community to design a more robust CHO cell line leading to the production of life saving medications in a more cost‐effective manner.
Biomanufacturing readiness levels [BRL]—A shared vocabulary for biopharmaceutical technology development and commercialization
Authors: Kedia Sandeep B. , Baker Jeffrey C. , Carbonell Ruben G. , Lee Kelvin H. , Roberts Christopher J. , Erickson John , Schiel John E. , Rogers Kelley , Schaefer Gene , Pluschkell Stefanie 
Published: September 2022

AbstractThe Manufacturing Readiness Levels (MRLs) developed by the Department of Defense are well‐established tools for describing the maturity of new technologies resulting from government‐sponsored Research and Development programs, from the concept phase to commercial deployment. While MRLs are generally applicable to a wide range of industries and technologies, there is significant value in offering an industry‐specific view on how the basic principles may be applied to biomanufacturing. This paper describes Biomanufacturing Readiness Levels (BRLs) developed by the National Institute for Innovation in Manufacturing Biopharmaceuticals (NIIMBL), a public/private partnership that is part of the Manufacturing USA network. NIIMBL brings together private, federal, nonprofit, and academic stakeholders to accelerate the deployment of innovative technologies for biopharmaceutical production and to educate and train a world‐leading biomanufacturing workforce. We anticipate that these BRLs will lay the groundwork for a shared vocabulary for assessment of technology maturity and readiness for commercial biomanufacturing that effectively meets the needs of this critical, specialized, and highly regulated industry.
Regional adaptation of the education in palliative and end‐of‐life Care Pediatrics (EPEC‐Pediatrics) curriculum in Eurasia
Authors: McNeil Michael J. , Ehrlich Bella , Yakimkova Taisiya , Wang Huiqi , Mishkova Volha , Bezler Zhanna , Kumirova Ella , Madni Arshia , Movsisyan Narine , Williams Karen , Baizakova Baglan , Borisevich Marina , Chatman Georgia , Erimbetova Indira , Quintero Ximena Garcia , Golban Rodica , Kirby Brandi , Nunez Paola , Ranadive Radhikesh , Sakhar Nadezhda , Sonnenfelt Jason , Volkova Alisa , Moreira Daniel , Friedrichsdorf Stefan J. , Wolfe Joanne , Remke Stacy , Hauser Joshua , Devidas Meenakshi , Baker Justin N. , Agulnik Asya 
Published: September 2022

AbstractBackgroundPediatric palliative care (PPC) is a priority to improve pediatric hematology oncology (PHO) care in Eurasia. However, there are limited regional opportunities for PPC education. We describe the adaptation and implementation of a bilingual end‐user Education in Palliative and End‐of‐Life Care (EPEC)‐Pediatrics course for PHO clinicians in Eurasia.MethodsDue to COVID‐19, this course was delivered virtually, consisting of prerecorded, asynchronous lectures, and a bilingual workshop with interactive lectures and small group sessions. A pre–postcourse design was used to evaluate the knowledge acquisition of the participants including their knowledge alignment with World Health Organization (WHO) guidance, ideal timing of palliative care, and comfort in providing palliative care to their patients. Questions were mostly quantitative with multiple choice or Likert scale options, supplemented by free‐text responses.ResultsA total of 44 (76%) participants from 14 countries completed all components of the course including pre‐ and postcourse assessments. Participant alignment with WHO guidance improved from 75% in the pre‐ to 90% in the postcourse assessments (p < 0.001). After participation, 93% felt more confident controlling the suffering of children at the end of life, 91% felt more confident in prescribing opioids and managing pain, and 98% better understood how to hold difficult conversations with patients and families. Most participants (98%) stated that they will change their clinical practice based on the skills and knowledge gained in this course.ConclusionsWe present a successful regional adaptation of the EPEC‐Pediatrics curriculum, including novel delivery of course content via a virtual bilingual format. This course resulted in significant improvement in participant attitudes and knowledge of PPC along with an understanding of the ideal timing of palliative care consultation and comfort in providing PPC to children with cancer. We plan to incorporate participant feedback to improve the course and repeat it annually to improve access to high‐quality palliative care education for PHO clinicians in Eurasia.
Integrated Metabolomics and Network Pharmacology to Reveal the Mechanisms of Guizhi‐Fuling Treatment for Myocardial Ischemia
Authors: Duan Bailu , Ye Yan , Zhou Zhenxiang , Han Lintao , Huang Fang , Li Jingjing , Wang Qiong , Zeng Xiangfa , Yu Xiaoming 
Published: September 2022

AbstractMyocardial ischemia is a cardio‐physiological condition due to a decrease in blood perfusion to the heart, leading to reduced oxygen supply and abnormal myocardial energy metabolism. Guizhi‐Fuling (GZFL) is effective in treating Myocardial ischemia. However, its mechanism of action is unclear and requires further exploration. We attempt to decipher the mechanisms behind GZFL treating Myocardial ischemia by integrating metabolomics and network pharmacology. In this study, myocardial metabolomic analysis was performed using GC/MS to identify the potential mechanism of action of GZFL during myocardial ischemia. Then, network pharmacology was utilized to analyze key pathways and construct a pathway‐core target network. Molecular docking was incorporated to validate core targets within network pharmacological signaling pathways. Finally, western blots were utilized to verify core targets of metabolomics, network pharmacology integrated pathways, and key signaling targets. Thus, 22 critical biomarkers of GZFL for treating myocardial ischemia were identified. Most of these metabolites were restored using modulation after GZFL treatment. Based on the network pharmacology, 297 targets of GZFL in treating myocardial ischemia were identified. The further comprehensive analysis focused on three key targets, such as Tyrosine hydroxylase (TH), myeloperoxidase (MPO), and phosphatidylinositol 3‐kinases (PIK3CA), and their related metabolites and pathways. Compared with the model group, the protein expression levels of TH, MPO and PIK3CA were reduced in GZFL. Therefore, the mechanism of GZFL for treating myocardial ischemia could inhibit myocardial inflammatory factors, reduce myocardial inflammation, and restore endothelial function while controlling norepinephrine release and uric acid concentration.
Co3O4 and CuBi2O4 Hybrid Nanostructures for Efficient Nonenzymatic Glucose Detection in Human Blood and Serum Samples with Mechanistic Insights
Authors: Gudipati Neeraja Sinha , Sinha Lichchhavi , Korutla Srikanth , Vanjari Sivaramakrishna , Tammineni Rajagopala Rao , Challapalli Subrahmanyam 
Published: September 2022

AbstractIn this report, CuBi2O4/Co3O4 has been used for the electrochemical detection of glucose. Herein, CuBi2O4 nanorods and Co3O4 has been hybridized with CuBi2O4 to form CuBi2O4/Co3O4 nanocomposite hydrothermally. CuBi2O4 and CuBi2O4/Co3O4 electrodes are fabricated by using drop casting method. Structural, morphological studies of CuBi2O4/Co3O4 and electrochemical characterizations pertaining to its performance in comparison to CuBi2O4 for electrochemical detection of glucose are performed and discussed elaborately. The detailed mechanism involved in the electrochemical detection of glucose is proposed. The electrode has shown the sensitivity of 49 μA mM−1 cm−2 with a limit of detection (LOD) of 0.136 μM. DFT calculations are performed on the optimized molecules involved in the electrochemical detection of glucose confirming CuBi2O4/Co3O4 acting as electron donor while glucose as electron acceptor. Further, natural bond orbital (NBO) for charge distribution calculations and ESP mappings for the optimized molecules are performed to understand nucleophilic/electrophilic positions. This study reveals that CuBi2O4 and its modifications are promising materials for the electrochemical detection of glucose. Further, the role of Co3O4 in improving the sensitivity and electrochemical performance for detection of glucose has been discussed.
Supramolecular Architecture of an Amphiphilic Amino Alcohol as a Versatile Chiral Environment for Stereocontrolled Photoreaction of Various Anthracenes
Authors: Kanai Hayato , Yamada Kuniyo , Salikolimi Krishnachary , Kodama Koichi , Ishida Yasuhiro 
Published: September 2022

AbstractThe photocyclodimerization of 2‐anthracenecarboxylic acid has been extensively studied as a model reaction of asymmetric photochemistry. So far, numerous chiral environments have been employed to control this photoreaction, while the scope of photoreactants has been limited only to 2‐anthracenecarboxylic acid and its simple esters and amides. Here, we developed a systematic series of photoreactants (2 a–d) by introducing various substituents to 2‐anthracenecarboxylic acid, which showed different reactivities and selectivities depending on the substituents. By using the photoreactants 2 a–d, we evaluated the performance of a chiral environment composed of an amphiphilic amino alcohol (1), where the photocyclodimerization of 2 a–d generally proceeded in excellent regio‐ and enantioselectivities (71–98 % regio ratio, 76–86 % ee). Furthermore, by reacting 2 a and 2 b together in the chiral environment of 1, we succeeded in the first stereocontrolled cross‐photocyclodimerization between two prochiral anthracenes (58 % chemo ratio, 83 % regio ratio, 90 % ee).