bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elemental analysis of single ambient aerosol particles using laser-induced breakdown spectroscopy
Authors: Heikkilä Paavo , Rostedt Antti , Toivonen Juha , Keskinen Jorma 
Published: August 2022

AbstractAnalysing the composition of aerosol particles is essential when studying their health effects, sources and atmospheric impacts. In many environments the relevant particles occur in very low concentrations, meaning that their analysis requires efficient single particle techniques. Here we introduce a novel method to analyse the elemental composition of single aerosol particles sampled directly from the aerosol phase using size amplification aided aerosol charging (SAAC), linear electrodynamic quadrupole (LEQ) and laser-induced breakdown spectroscopy. We present results of the charging and focusing efficiencies of the SAAC and of the LEQ, and a proof-of-concept of the analysis method. The proof-of-concept test series was conducted with particle diameters down to 300 nm, sampled directly from the aerosol phase. The method shows unprecedented performance for spectroscopic submicron particle analysis from arbitrarily low concentrations and has exceptional potential for a portable analysis platform for various applications in the field of aerosol research.
High-efficiency decomposition of eggshell membrane by a keratinase from Meiothermus taiwanensis
Authors: Lien Ya-Chu , Lai Shu-Jung , Lin Chai-Yi , Wong Ken-Pei , Chang Matt S. , Wu Shih-Hsiung 
Published: August 2022

AbstractEggshell membrane (ESM), a plentiful biological waste, consists of collagen-like proteins and glycosaminoglycans (GAGs) such as hyaluronic acid (HA). Here we used a keratinase (oeMtaker)-mediated system to decompose ESM. The best reaction condition was established by incubating the solution containing oeMtaker, sodium sulfite, and ESM with a weight ratio of 1:120:600. ESM enzymatic hydrolysate (ESM-EH) showed a high proportion of essential amino acids and type X collagen peptides with 963–2259 Da molecular weights. The amounts of GAGs and sulfated GAGs in ESM-EH were quantified as 6.4% and 0.7%, respectively. The precipitated polysaccharides with an average molecular weight of 1300–1700 kDa showed an immunomodulatory activity by stimulating pro-inflammatory cytokines (IL-6 and TNF-α) production. In addition, a microorganism-based system was established to hydrolyze ESM by Meiothermus taiwanensis WR-220. The amounts of GAGs and sulfated GAGs in the system were quantified as 0.9% and 0.1%, respectively. Based on our pre-pilot tests, the system shows great promise in developing into a low-cost and high-performance process. These results indicate that the keratinase-mediated system could hydrolyze ESM more efficiently and produce more bioactive substances than ever for therapeutical applications and dietary supplements.
Ultrafast laser inscribed waveguides in tailored fluoride glasses: an enabling technology for mid-infrared integrated photonics devices
Authors: Fernandez Toney T. , Johnston B. , Gross S. , Cozic S. , Poulain M. , Mahmodi H. , Kabakova I. , Withford M. , Fuerbach A. 
Published: August 2022

AbstractZirconium fluoride (ZBLAN) glass, the standard material used in fiber-based mid-infrared photonics, has been re-designed to enable the fabrication of high index-contrast low-loss waveguides via femtosecond laser direct writing. We demonstrate that in contrast to pure ZBLAN, a positive index change of close to 10−2 can be induced in hybrid zirconium/hafnium (Z/HBLAN) glasses during ultrafast laser inscription and show that this can be explained by an electron cloud distortion effect that is driven by the existence of two glass formers with contrasting polarizability. High numerical aperture (NA) type-I waveguides that support a well confined 3.1 μm wavelength mode with a mode-field diameter (MFD) as small as 12 μm have successfully been fabricated. These findings open the door for the fabrication of mid-infrared integrated photonic devices that can readily be pigtailed to existing ZBLAN fibers.
The implications of crustal architecture and transcrustal upflow zones on the metal endowment of a world-class mineral district
Authors: Jørgensen Taus R. C. , Gibson Harold L. , Roots Eric A. , Vayavur Rajesh , Hill Graham J. , Snyder David B. , Naghizadeh Mostafa 
Published: August 2022

AbstractEarth’s mineral deposits show a non-uniform spatial distribution from the craton-scale, to the scale of individual mineral districts. Although this pattern of differential metal endowment is underpinned by lithospheric-scale processes the geological features that cause clustering of deposits remains enigmatic. The integration of geological and geophysical (seismic, gravity, and magnetotelluric) features has produced the first whole-of-crust image through an iconic Neoarchean volcanic complex and mineral district in the Abitibi Greenstone Belt, Superior Province, Canada. Observations indicate an asymmetry in surface geology, structure, and crustal architecture that defines deep transcrustal magmatic-hydrothermal upflow zones and the limits of the Noranda District ore system. Here, extreme volcanogenic massive sulfide (VMS) endowment is confined to a smaller area adjacent to an ancestral transcrustal structure interpreted to have localized and optimized magmatic and ore forming processes. Although lithospheric-scale evolutionary processes might act as the fundamental control on metal endowment, the new crustal reconstruction explains the clustering of deposits on both belt and district scales. The results highlight a strong magmatic control on metal and in particular Au endowment in VMS systems. Overprinting by clusters of ca. 30 Ma younger orogenic Au deposits suggest the ore systems accessed an upper lithospheric mantle enriched in Au and metals.
Improved cognitive performance in trace amine-associated receptor 5 (TAAR5) knock-out mice
Authors: Maggi Silvia , Bon Carlotta , Gustincich Stefano , Tucci Valter , Gainetdinov Raul R. , Espinoza Stefano 
Published: August 2022

AbstractTrace amine-associated receptors (TAARs) are a family of G protein-coupled receptors present in mammals in the brain and several peripheral organs. Apart from its olfactory role, TAAR5 is expressed in the major limbic brain areas and regulates brain serotonin functions and emotional behaviours. However, most of its functions remain undiscovered. Given the role of serotonin and limbic regions in some aspects of cognition, we used a temporal decision-making task to unveil a possible role of TAAR5 in cognitive processes. We found that TAAR5 knock-out mice showed a generally better performance due to a reduced number of errors and displayed a greater rate of improvement at the task than WT littermates. However, task-related parameters, such as time accuracy and uncertainty have not changed significantly. Overall, we show that TAAR5 modulates specific domains of cognition, highlighting a new role in brain physiology.
Aberrant gene expression of superoxide dismutases in Chlamydia trachomatis-infected recurrent spontaneous aborters
Authors: Ray Ankita , Bhati Tanu , Pradhan Dibyabhaba , Arora Renu , Parvez Suhel , Rastogi Sangita 
Published: August 2022

AbstractStudy aimed to characterize the expression of antioxidant genes SOD1 and SOD2 in Chlamydia trachomatis-induced recurrent spontaneous aborters and further determine their role by in silico analysis. First void urine was collected from 130 non-pregnant women with history of recurrent spontaneous abortion (RSA) (Group I) and 130 non-pregnant women (Group II; control) attending Obstetrics and Gynecology Department, SJH, New Delhi, India. C. trachomatis detection was performed by conventional PCR in urine. Gene expression of SOD1 and SOD2 was performed by quantitative real-time PCR. Further, its interacting partners were studied by in silico analysis. 22 patients were positive for C. trachomatis in Group I. Significant upregulation was observed for SOD2 gene in C. trachomatis-infected RSA patients while SOD1 was found to be downregulated. Increased concentration of oxidative stress biomarkers 8-hydroxyguanosine and 8-isoprostane was found in C. trachomatis-infected RSA patients. Protein–protein interaction (PPI) of SOD proteins and its interacting partners viz.; CCS, GPX1, GPX2, GPX3, GPX4, GPX5, GPX7, GPX8, CAT, PRDX1, TXN, SIRT3, FOXO3, and AKT1 were found to be involved in MAPK, p53 and foxo signaling pathways. Molecular pathways involved in association with SODs indicate reactive oxygen species (ROS) detoxification, apoptotic pathways and cell cycle regulation. Overall data revealed alleviated levels of SOD2 gene and decreased expression of SOD1 gene in response to C. trachomatis-infection leading to production of oxidative stress and RSA.
A numerical study on CO migration after blasting in high-altitude tunnel by inclined shaft
Authors: Wu Bo , Zhao Rui , Meng Guowang , Xu Shixiang , Qiu Weixing , Chen Huihao 
Published: August 2022

AbstractOn the western plateau of China, ventilation problems brought on by low atmospheric pressure must be overcome. And CO migration after blasting in high-altitude tunnel by inclined shaft has become a significant scientific issue. In this study, the Computational Fluid Dynamics (CFD) method was used to analyze the flow field characteristics at the junction of the inclined shaft and tunnel. In addition, the effects of different fan opening modes and different initial CO concentration distributions on the ventilation were discussed. The simulation results showed that the main difference in the ventilation wind field was reflected in the position of the vortex region due to the different fan opening modes. Meanwhile, various initial CO concentration distributions showed different migration when there was no air volume difference between the left and right tunnels. Eliminating vortex zones and fully using high velocity airflow could improve relative ventilation efficiency by at least 18%. CO would accumulate in the opposite direction of the tunnel if only one of the fans was turned on. Therefore, a two-stage ventilation scheme was proposed, and the energy consumption was reduced by at least 33%. This research can provide guidance on high-altitude tunnel construction with multiple working faces to improve ventilation efficiency and reduce energy consumption.
Disease-related protein co-expression networks are associated with the prognosis of resectable node-positive pancreatic ductal adenocarcinoma
Authors: Nishimura Toshihide , Takadate Tatsuyuki , Maeda Shimpei , Suzuki Takashi , Minowa Takashi , Fukuda Tetsuya , Bando Yasuhiko , Unno Michiaki 
Published: August 2022

AbstractPancreatic ductal adenocarcinoma (PDAC) is a multifactorial disease, the molecular profile of which remains unclear. This study aimed at unveiling the disease-related protein networks associated with different outcomes of resectable, node-positive PDAC cases. We assessed laser-microdissected cancerous cells from PDAC tissues of a poor outcome group (POG; n = 4) and a better outcome group (BOG; n = 4). Noncancerous pancreatic duct tissues (n = 5) were used as the reference. We identified four representative network modules by applying a weighted network correlation analysis to the obtained quantitative PDAC proteome datasets. Two network modules that were significant for POG were associated with the heat shock response to hypoxia-related stress; in the latter, a large involvement of the non-canonical Hedgehog pathway (regulated by GLI1), the internal ribosome entry site-mediated cap-independent translation, the inositol requiring enzyme 1-alpha (IRE1α)/X-box binding protein 1 pathway of the unfolding protein response (UPR), and the aerobic glycolysis was observed. By contrast, the BOG characteristic module was involved in the inactivation of the UPR pathway via the synoviolin 1-dependent proteasomal degradation of IRE1α, the activation of SOX2, and the loss of PALB2 (partner and localizer of BRCA2) function, all potentially suppressing malignant tumor development. Our findings might facilitate future therapeutic strategies for PDAC.
Multimodal integration of radiology, pathology and genomics for prediction of response to PD-(L)1 blockade in patients with non-small cell lung cancer
Authors: Vanguri Rami S. , Luo Jia , Aukerman Andrew T. , Egger Jacklynn V. , Fong Christopher J. , Horvat Natally , Pagano Andrew , Araujo-Filho Jose de Arimateia Batista , Geneslaw Luke , Rizvi Hira , Sosa Ramon , Boehm Kevin M. , Yang Soo-Ryum , Bodd Francis M. , Ventura Katia , Hollmann Travis J. , Ginsberg Michelle S. , Gao Jianjiong ,   MSK MIND Consortium, Vanguri Rami , Hellmann Matthew D. , Sauter Jennifer L. , Shah Sohrab P. 
Published: August 2022

AbstractImmunotherapy is used to treat almost all patients with advanced non-small cell lung cancer (NSCLC); however, identifying robust predictive biomarkers remains challenging. Here we show the predictive capacity of integrating medical imaging, histopathologic and genomic features to predict immunotherapy response using a cohort of 247 patients with advanced NSCLC with multimodal baseline data obtained during diagnostic clinical workup, including computed tomography scan images, digitized programmed death ligand-1 immunohistochemistry slides and known outcomes to immunotherapy. Using domain expert annotations, we developed a computational workflow to extract patient-level features and used a machine-learning approach to integrate multimodal features into a risk prediction model. Our multimodal model (area under the curve (AUC) = 0.80, 95% confidence interval (CI) 0.74–0.86) outperformed unimodal measures, including tumor mutational burden (AUC = 0.61, 95% CI 0.52–0.70) and programmed death ligand-1 immunohistochemistry score (AUC = 0.73, 95% CI 0.65–0.81). Our study therefore provides a quantitative rationale for using multimodal features to improve prediction of immunotherapy response in patients with NSCLC using expert-guided machine learning.
Hearing Health Care Digital Therapeutics: Patient Satisfaction Evidence
Authors: Tye-Murray Nancy , Spehar Brent , Mauze Elizabeth , Cardinal Christopher 
Published: August 2022

Purpose:
A digital therapeutic is a software-based intervention for a disease and/or disorder and often includes a daily, interactive curriculum and exercises; online support from a professional versed in the treatment base; and an online support community, typically active as a social chat group. Recently, the Consumer Technology Association published revised standards for digital therapeutics (DTx) that stipulate that a DTx must be evidence based and founded in scientific evidence showing effectiveness and must be supported by evidence showing improved patient satisfaction and adherence to an intervention. The purpose of this study was to investigate whether a DTx could help older adults better adjust to their hearing loss and acclimate to new hearing aids.

Method:
Thirty older adults with mild or moderate hearing loss who had never used hearing aids participated. All hearing aids were fitted remotely. Participants used a hearing health care DTx (Amptify) for 4 weeks, either immediately following receipt of the hearing aids or 4 weeks after the fitting. A control condition was watching closed caption television. Participants completed a satisfaction questionnaire that queried about their impressions of the DTx, which had items that included both a rating scale of 1–7 and open-ended questions.

Results:
Ninety-six percent of the participants reported positive benefits, and one-half reported that the DTx helped them to adjust to their new hearing aids. They assigned a score of 5.8 to one of the questionnaire items that is similar to a Net Promoter Score Benefits, which included an enhanced ability to engage in conversation and increased listening confidence.

Conclusion:
This investigation provides scientific evidence to support the use of a hearing health care DTx, paving the way for audiologists to be able to more easily and efficiently incorporate follow-up aural rehabilitation into their routine clinical services and to be able to provide services remotely.
Auditory Experience, Speech Sound Production Growth, and Early Literacy in Children Who Are Hard of Hearing
Authors: Farquharson Kelly , Oleson Jacob , McCreery Ryan W. , Walker Elizabeth A. 
Published: August 2022

Purpose:
Children who are hard of hearing (CHH) are at risk for literacy difficulties. Speech sound production is related to literacy skills in typically hearing children. Speech sound production abilities can also be markedly weak in CHH due to inconsistent access to the speech signal. We longitudinally examined relations between auditory experience, speech sound production abilities, and literacy (i.e., nonword reading and spelling in second grade) in CHH.

Method:
Participants included 166 CHH. All participants used hearing aids (HAs). Auditory experience is a weighted measure of the number of hours of daily HA use and the amount of audibility with and without their HAs. Children's speech sound production was tested 2–3 times between the ages of 3 and 9 years. At age 5 years and again in second grade, children were tested on a battery of language and literacy measures.

Results:
Auditory experience was significantly correlated with speech sound production abilities, but age at HA fitting was not. Speech sound production abilities at age 5 years did not predict nonword reading in second grade but did predict spelling skills in second grade.

Conclusions:
Our results support the importance of auditory experience and speech sound production for later literacy abilities. Specifically, we found that speech sound production abilities and print knowledge at age 5 years are related to second grade spelling outcomes. Interestingly, we found that morphological knowledge at age 5 years was more predictive of decoding in second grade than was speech sound production. Although there are multiple factors that contribute to literacy success, our results underscore the unique contributions of speech sound production abilities in CHH.
Non‐chromate conversion process for zinc coating with durable hydrophobicity and enhanced corrosion resistance
Authors: Chen J. Y. , Hu Z. Y. , Li Z. X. , Wang X. L. , Gu C. D. , Tu J. P. 
Published: August 2022

AbstractA Zn‐based coating with durable hydrophobicity and good corrosion resistance was formed on a mild steel substrate, which involves electroplating Zn from a non‐aqueous electrolyte, followed by passivation in an oleic acid (OA) solution. The electrodeposited Zn coatings were porous, which facilitated the formation of a chemical conversion layer of Zn oleate (ZO) during OA passivation. The Zn coating after passivation had a two‐layer structure, which included an outer layer of ZO with a thickness of ∼26 μm and an inner layer of Zn with a thickness of ∼6 μm. The outer layer ZO is a type of metal soap with a smooth surface and durable hydrophobicity, such that water droplets can easily slip off its surface. Corrosion testing and electrochemical measurements in 3.5 wt.% NaCl aqueous solution indicate that the Zn coating after OA passivation exhibits outstanding anti‐corrosion properties compared with those exhibited by pure Zn coating. The corrosion products and mechanism of the two‐layer coating were explored. This study shows that smooth metal oleate coatings can provide hydrophobicity and corrosion resistance simultaneously to mild steel substrates.
The formation of CO2 through consumption of gas-phase CO on vacuum-UV irradiated water ice
Authors: Terwisscha van Scheltinga J. , Ligterink N. F. W. , Bosman A. D. , Hogerheijde M. R. , Linnartz H. 
Published: August 2022

Context. Recent observations of protoplanetary disks suggest that they are depleted in gas-phase CO up to a factor of 100 with respect to predictions from physical-chemical (or thermo-chemical) models. It has been posed that gas-phase CO is chemically consumed and converted into less volatile species through gas-grain processes. Observations of interstellar ices reveal a CO2 component in a polar (H2O) ice matrix, suggesting potential co-formation or co-evolution.
Aims. The aim of this work is to experimentally verify the interaction of gas-phase CO with solid-state OH radicals on the surface of water ice above the sublimation temperature of CO.
Methods. Amorphous solid water (ASW) is deposited in an ultra-high vacuum (UHV) setup at 15 K and irradiated with vacuum-UV (VUV) photons (140–170 nm, produced with a microwave-discharge hydrogen-flow lamp) to dissociate H2O and create OH radicals. Gas-phase CO is simultaneously admitted and only adsorbs with a short residence time on the ASW. Formed products in the solid state are studied in the infrared through Fourier transform infrared spectroscopy and once released into the gas phase with quadrupole mass spectrometry.
Results. Our experiments show that gas-phase CO is converted into CO2 when interacting with ASW that is VUV irradiated with a conversion efficiency of 7–27%. Between 40 and 90 K, CO2 production is constant, above 90 K, CO2 production is reduced in favor of O2 production. In the temperature range of 40-60 K, the CO2 remains in the solid state, while at temperatures 70 K the majority of the formed CO2 is immediately released into the gas phase.
Conclusions. We conclude that gas-phase CO reacts with OH radicals, created on the surface of ASW with VUV irradiation, above its canonical sublimation temperature. The diffusion during the short, but nonzero, residence times of CO on the surface of ASW suggests that a Langmuir-Hinshelwood type reaction is involved. This gas-phase CO and solid-state OH radical interaction could explain (part of) the observed presence of CO2 embedded in water-rich ices when it occurs during the build up of the H2O ice mantle. It may also contribute to the observed lack of gas-phase CO in planet-forming disks, as previously suggested. It should be noted though that our experiments indicate a lower water ice dissociation efficiency than originally adopted in model descriptions of planet-forming disks and molecular clouds. Incorporation of the reduced water ice dissociation and increased binding energy of CO on a water ice surfaces in physical-chemical models would allow investigation of this gas-grain interaction to its full extend.
A robust model for the origin of optical quasi-periodic variability in supersoft X-ray sources
Authors: Zhao Weitao , Meng Xiangcun , Cui Yingzheng , Liu Zheng-Wei 
Published: August 2022

Context. Supersoft X-ray sources (SSSs) are known as possible progenitors of Type Ia supernovae. The quasi-periodic variability has been detected in the optical light curves of SSSs. However, the exact origin of such quasi-periodic observable features remains a mystery.
Aims. In this paper, we aim to reproduce the observed optical quasi-periodic variability of SSSs by proposing a white dwarf (WD) accretion model with a periodic mass transfer caused by the irradiation of supersoft X-ray onto the companion star.
Methods. Assuming that a periodic mass transfer from the companion star to the WD can be caused while the supersoft X-ray irradiates the companion star, we used MESA to simulate the WD accretion process and the subsequent WD evolution by adopting a periodic jagged accretion rate.
Results. Comparing our results to the optical light curves of a well-observed SSS RX J0513.9-6951, we find that our models can reproduce the quasi-periodic transition between the optical high and low states of RX J0513.9-6951 because the periodic accretion rate can lead to the WD photosphere expands and contracts periodically in our models. In addition, we find that the transitional periods of the SSSs in our models strongly depend on the mass of the accreting WDs. The more massive the WD mass is, the shorter the transitional period.
Conclusions. Based on our results, we suggest that the periodic mass transfer caused by the irradiation of supersoft X-ray onto the companion star may be the origin of the observed optical quasi-periodic variability in SSSs. In addition, our results indicate that the observed optical transition period of a SSS may be useful for the rough estimate of the mass of an accreting WD.