bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gas emission characteristics of tectonic coal in the initial stage: Application for coal and gas outburst prediction
Authors: Zhang Huijie , Huo Zhonggang , Shu Longyong , Li Qixian , Liu Zhengshuai 
Published: June 2022

Abstract
Coal and gas outburst is one of the most disasters threating the safety and production of coal mines. Accurate description of the gas emission characteristics in the initial stage for tectonic coal is the key to improving the prediction accuracy of coal and gas outburst. In this work, the gas emission test of original and tectonic coal particles under the constant temperature was carried out. The optimal mathematical model suitable for the gas emission in the initial stage was then analyzed. Moreover, the time influencing factors of the optimal mathematical model were discussed. The research results show that cumulative gas desorption amount during 0-7 minute for tectonic coal is 1.28-1.41 times as much as that for original coal. The applicability of current mathematical model for describing the gas emission characteristics of tectonic coal in the initial stage has limitations. Five typical mathematical expressions are selected and compared to fit gas emission curve in the initial stage. Considering the sensitivity of parameters in outburst prediction, the optimal mathematical model was obtained. In addition, the time factor has a great influence on the application effect of mathematical model in coal and gas outburst prediction. For current mathematical model, the errors of tectonic coal are all negative. The greater the gas pressure and the later the measurement time, the greater the error. The errors in 1~6 min are -0.46%~-1.78%, and the errors in 2~7 min are -1.00%~-3.86%, which is not conducive to the safety of outburst prediction. Therefore, the optimal mathematical model has the possibility to overcome the limitations of current mathematical model. The research results provide a theoretical basis for the correction of coal and gas outburst prediction indicators.
Hyperledger Fabric Consensus Optimization Method Based on Endorsing Node
Authors: Wang Zhurong , Zhu Min , Wang Yichuan , Hei Xinhong 
Published: June 2022

Abstract

Aiming at a series of security risks caused by the transparency and scarcity of endorsing nodes information in Hyperledger fabric, a non-interactive and verifiable optimization scheme for randomly selecting endorsing nodes is proposed, that is, the fabric model based on endorsement-ordering-verification. By introducing the candidate set of endorsing nodes and using the verifiable random function based on the elliptic curve SM2 algorithm to randomly select endorsing nodes as transactions for endorsement strategy, the model realizes the non-interactive random selection function of endorsing nodes in fabric, which can effectively prevent hacker attacks on the network. Experimental results show that the proposed scheme has higher security than the comparison method and the scheme before optimization.
Senegenin rescues PC12 cells with oxidative damage through inhibition of ferroptosis
Authors: Zhang Heping , Zhou Wei , Li Jianling , Qiu Zhaohui , Wang Xiaotong , Xu Hui , Wang Huadong , Lu Daxiang , Qi Renbin 
Published: June 2022

Abstract
Oxidative stress is one of the pathological mechanisms of Alzheimers disease (AD), and ferroptosis has been determined to be involved in neurodegenerative diseases such as AD. Senegenin (Sen) prevents oxidative damage in nerve cells via a mechanism that may be highly related to ferroptosis. However, the mechanism of ferroptosis pathway involvement in AD is unclear. In this study, we established a model of PC12 cytotoxic injury induced by Aβ25−35, and we detected the level of oxidative damage, MMP, and ferroptosis-related proteins expression. The results showed that, compared with control group, the level of ROS increased, GPX activities decreased, and MDA levels increased in Aβ25−35 group. Aβ25−35 could induce mitochondrial depolarization in PC12 cells and Fer-1 could not reverse this damage. WB revealed that Aβ25−35 group had increased ACSL4 and PEBP1 proteins, and decreased GPX4 protein. After adding Sen in the model, the level of oxidative damage was reduced, and mitochondrial depolarization was reversed compared with Aβ25−35 group. WB suggested that the expression of ACSL4 and PEBP1 proteins decreased, and the expression of GPX4 protein increased by Sen treatment. In conclusion, we found that Sen exhibits strong neuroprotective activity against Aβ25−35 induced oxidative damage and lipid metabolic associated with ferroptosis. Inhibiting nerve cells ferroptosis might facilitate the future development of strategies to AD.
MedGAN-Net:a computer-aided diagnosis approach based on deep learning for COVID-19 with CT images
Authors: Nie Shanling , Cai Yunjie , Guo Yue , Zheng Zeqi , Yang Hai 
Published: June 2022

Abstract
Since the outbreak of COVID-19, novel pneumonia has put massive pressure on medical systems globally. The computer-aided diagnosis(CAD) approach could improve the efficiency of the diagnosis process, thus relieving the pressure. This study proposed a complete CAD approach named MedGAN-Net for COVID-19 diagnosis based on CT images. MedGAN-Net includes a novel data augmentation method and a classification module. Based on the modified TransGAN and CNN, the data augmentation method is to generate more positive samples as training data. The ResNet50 is adopted as the classification model. Evaluated on the benchmark dataset, the classification results of MedGAN-Net prove to be more accurate than other models. And our data augmentation method also outperformed other typical methods. Based on visualization, we analyzed the interpretability of MedGAN-Net and found it successfully caught the manifestations of COVID-19 on CT images. According to the overall assessment, the MedGAN-Net showed the possibility of assisting clinical diagnosis based on CT images.
CRISPR-Mediated Generation and Characterization of a Gaa Homozygous c.1935C&gt;A (p.D645E) Pompe Disease Knock-in Mouse Model Recapitulates Human Infantile Onset-Pompe Disease
Authors: Kan Shih-hsin , Huang Jeffrey Y. , Harb Jerry , Rha Allisandra , Dalton Nancy D. , Christensen Chloe , Chan Yunghang , Davis-Turak Jeremy , Neumann Jonathan , Wang Raymond Y. 
Published: June 2022

Abstract
Pompe disease, an autosomal recessive disorder caused by deficient lysosomal acid α-glucosidase (GAA), is characterized by accumulation of intra-lysosomal glycogen in skeletal and oftentimes cardiac muscle. The c.1935C>A (p.Asp645Glu) variant, the most frequent GAA pathogenic mutation in people of Taiwanese and Southern Chinese ethnicity, causes infantile-onset Pompe disease (IOPD), presenting neonatally with severe hypertrophic cardiomyopathy, profound muscle hypotonia, respiratory failure, and infantile mortality. We applied CRISPR-Cas9 homology-directed repair (HDR) using a novel dual sgRNA approach flanking the target site to generate a GaaEm1935C>A knock-in mouse model and a myoblast cell line carrying the Gaa c.1935C>A mutation. Herein we describe the molecular, biochemical, physiological, histological, and behavioral characterization of 3-month-old homozygous GaaEm1935C>A mice. Homozygous GaaEm1935C>A knock-in mice exhibited normal Gaa mRNA expression levels relative to wild-type mice, had near-abolished GAA enzymatic activity, markedly increased tissue glycogen storage, and concomitantly impaired autophagy. Three-month-old mice demonstrated skeletal muscle weakness and hypertrophic cardiomyopathy but no premature mortality. The GaaEm1935C>A knock-in mouse model recapitulates multiple salient aspects of human IOPD caused by the GAA c.1935C>A pathogenic variant. It is an ideal model to assess innovative therapies to treat IOPD, including personalized therapeutic strategies that correct pathogenic variants, restore GAA activity and produce functional phenotypes.
Financial Impacts and Community Resources Utilization of Children with Feeding Difficulties
Authors: Okada June , Wilson Erin , Wong John , Luo Man , Fiechtner Lauren , Simione Meg 
Published: June 2022

Abstract
Background
To examine the extent to which financial impacts and community resources utilization are associated with pediatric feeding difficulties. We hypothesize that children with feeding difficulties will have more financial impacts and community resources utilization than children without feeding difficulties.
Methods
We conducted a secondary analysis of cross-sectional data from the 2017–2018 National Survey of Children’s Health regarding 14,960 children 0–5 years. Outcomes included out-of-pocket costs, caregivers leaving a job due to the child’s health, food insufficiency, receival of food or cash assistance, and receival of special education and/or developmental services. We used a multivariable logistic regression controlling for sociodemographic factors to examine the associations of feeding difficulties with financial impacts and community resources utilization outcomes.
Results
Out of 14,690 respondents, 1.7% of children reported feeding difficulties. These children had higher odds of having out-of-pocket costs of ≥$1000 (OR: 3.01; 95% CI: 1.61, 5.62), having a caregiver that left a job due to their child’s health (OR: 3.16; 95% CI: 2.01, 4.98), experiencing food insufficiency (OR: 1.67; 95% CI: 1.03, 2.71), and receiving special education and/or developmental services (OR 3.98; 95% CI: 2.46, 6.45) than children without feeding difficulties.
Conclusions
Children with feeding difficulties are more likely to have financial impacts and community resources utilization than children without feeding difficulties. This information can be used to tailor interventions to improve family-centered care and outcomes for children.
Calcaneous interlock-nail treatment for Calcaneous fracture: a multiple center retrospective study
Authors: Peng Ye , Wang Junsong , Feng Bo , Li Yunshou , Zhu Yunlong , Yuan Weiqing , Teng Lei , Zhu Chengming , Shi Bin , Zhang Lihai 
Published: June 2022

Abstract
Background
Minimally invasive treatments for calcaneous fractures have the same outcomes and fewer complications. However, they are technically demanding, and there are a lack reduction tools. To overcome these problems, a calcaneous interlock nail system was developed that can make reduction and fixation minimally invasive and effective. We retrospectively studied the calcaneous fracture variables intraoperatively and followed up to evaluate the outcomes of patients treated with the interlock nail system.
Methods
All patients in 7 institutions between October 2020 and May 2021 who had calcaneous fractures treated with interlock nails were retrospectively analyzed. The patient characteristics, including age, sex, injury mechanism, Sanders type classification, smoking status, and diabetes, were recorded. The interlock calcaneous nail and standard surgical technique were introduced. The intraoperative variables, including days waiting for surgery, surgery time, blood loss, incision length, and fluoroscopy time, were recorded. The outcomes of complications, AOFAS scores and VAS scores were recorded.
Results
Fifty-nine patients were involved in this study; 54 were male; 5 were female; and they had an average age of 47.5 ± 9.2 years (range 25–70). Two of these fractures were Sanders type I, 28 of these fractures were Sanders type II, 27 of these fractures were Sanders type III, and 2 of these were Sanders type IV. The surgery time was 131.9 ± 50.5 (30–240) minutes on average. The blood loss was 36.9 ± 41.1 (1-250). The average incision length was 3.5 ± 1.8 (1–8) cm; 57 were sinus tarsi incisions; and 2 were closed fixations without incisions. The average fluoroscopy time was 12.3 ± 3.6 (10–25) seconds during the surgery. The VAS score of patients on the day after surgery was 2.4 ± 0.7 (1–3). The AOFAS ankle-hindfoot score in patients who had a follow-up of at 12 months was 93.3 ± 3.6(85–99). During the follow-up, all patients’ functional outcomes were good. One patient had a superficial infection. The rate of complications of the 59 patients was 1.7% (1/59).
Conclusions
The calcaneous interlock nail system can have satisfactory reduction and fixation in calcaneous fractures, even in Sanders type IV. The outcomes of follow-up showed good function. The interlock nail could be an alternative method for minimally invasive calcaneous fracture fixation.
Rotational Stability and Clinical Effects of Different Toric ICL Placements: A Case- Control Study
Authors: zhu qiu-jian , Xing Xiao-Ying , Zhu Wei-Jian , Zhu Man-Hui , Ma Lie , Yuan You 
Published: June 2022

Abstract
Background
To compare the visual and refractive outcomes, vault, and rotational stability between horizontal and vertical toric implantable collamer lens (ICL) placement.
Methods
This retrospective case-control study comprised 36 eyes of 36 patients with vertical toric ICL placement and 36 eyes of 36 patients with horizontal placement. The two groups were matched strictly 1:1 from January 2020 to October 2020. The visual acuity, vault, manifest refraction, efficacy index (EI), safety index (SI), vector analysis, and rotational stability were compared between the two groups.
Results
Residual astigmatism in the vertical group was slightly smaller than that in the horizontal group (P = 0.024), and the index of success (IOS) was slightly greater in the vertical group than in the horizontal group (P = 0.013). Furthermore, the vault was significantly higher in the horizontal placement group than in the vertical placement group (P < 0.001). Compared to the preoperative design, the absolute degree of rotation at 3 months postoperatively was 3.44° ± 2.72° for the horizontal group and 4.83° ± 2.93° for the vertical group. However, if the reference was changed to 2 hours postoperatively, the absolute rotation degree was 1.81° ± 1.84° for the horizontal placement group and 1.97° ± 1.70° for the vertical placement group. There was no significant difference in other parameters between the two groups.
Conclusions
Vertical placement of a toric ICL can have clinical effects and rotational stability as good as those with horizontal placement. Moreover, compared with horizontal placement, vertical placement can effectively reduce vault.
A retrospective study of the risk factors for linezolid - induced haematological toxicity in Chinese patients
Authors: Mo Kai , Cao Wen , Lu YaTing , Qin YanE , Li JuMan , Liang YingE , Wei RuHua , Zhong Hui , Xiaobu Lan 
Published: June 2022

Abstract
Purpose: To investigate the risk factors of linezolid-induced haematological toxicity in hospitalized patients.Methods: In this retrospective, single-center, observational cohort study, patients underwent linezolid TDM trough concentration (Cmin) during treatment between January 2020 and December 2021 were analysed.Univariate and multivariate logistic regression analysis were used to investigate the relevant risk variables, and a relative operating characteristic (ROC) curve was generated to predict clinical characteristics. Results: A total of 111 valid individuals were studied, among which 47 were diagnosed with linezolidine-related thrombocytopenia and 18 were diagnosed with linezolidine-related hemoglobin decrease, respectively. Binary logistic regression analysis showed that creatinine clearance level (Ccr) <50 ml/min/1.73 m2 [OR, 5.463; 95% Cl, 1.249-23.888, P=0.024] and the plasma trough concentration >7mg/L [OR, 62.660; 95% Cl, 14.293-274.708, P=0.001] were risk factors associated with thrombocytopenia induced by linezolid. The area under the ROC curve of linezolid plasma trough concentration was 0.955, the Youden index was 0.837 at the maximum, and the corresponding critical value was 6.94 mg/L, with a sensitivity of 91.5% and a specificity of 92.2%. Moreover, Ccr <50 ml/min/1.73 m2 [OR, 7.282; 95% Cl, 1.765-30.048, P=0.006] and the plasma trough concentration >7mg/L[OR, 6.364; 95% Cl, 1.937-20.910, P=0.020] were closely related to linezolid related hemoglobin reduction. The area under the ROC curve of linezolid plasma trough concentration was 0.755, the Youden index was 0.477 at the maximum, and the corresponding critical value was 7.53 mg/L, with a sensitivity of 77.8% and a specificity of 69.9%. Conclusion: Renal insufficiency is a related risk factor for linezolid-induced haematological toxicity. It is recommended that patients treated with linezolid should monitor blood routine and plasma concentration, especially in patients with moderate or severe renal insufficiency. Plasma trough concentration of linezolid could be a suitable predictor for linezolid-related thrombocytopenia and anemia.
An effective selenium-based fluorescence chemosensor for selective recognition of Hg2+ in aqueous medium: experimental and theoretical studies
Authors: - Puthiyavalappil Rasin , - Moideen Musthafa , Sreekant Anandaram 
Published: June 2022

Abstract
We introduce a novel selenium-based compound [N-(Phenylcarbamoselenoyl) furan-2-carboxamide] for the optical and fluorimetric detection of Hg in an aqueous medium. The synthesized compound was characterized by different spectroscopic methods. The designed chemosensor FSU has shown a significant fluorescence quenching when Hg2+ ions were added to the sensing medium. Furthermore, Hg2+ ions provoked a 2:1 complex formation with the chemosensor FSU. It is found that the compound offers high selectivity over a variety of cations such as Co2+, Cr3+, Ni2+, Zn2+, Cu2+, Mg2+, Hg2+, Cd2+, Ca2+, Mn2+, Ga3+, Pb2+, Na+, Fe2+ and K+. The detection limit was calculated as 7.35×10-7 M. Also, FSU shows appreciable binding affinity towards Hg2+ ions with a binding constant value of 1.413×103 M-1. The ICT mechanism of mercury sensing was confirmed with spectroscopic techniques and DFT studies. Density functional theory was also implemented to investigate the structure of the Hg2+ complex and its electronic distribution in the aqueous medium. Finally, an MEP study was also carried out to obtain detailed information about the surface characteristics of the chemosensor FSU. Effectively, we have reported a potent chemosensor for Hg2+ in the aqueous medium.
Abrasive fluidized bed finishing of additive manufactured cobalt-chrome parts: effects on surface morphology and fatigue behaviour
Authors: Atzeni Eleonora , Genna Silvio , Salmi Alessandro , Trovalusci Federica , Rubino Gianluca 
Published: June 2022

Abstract
The possibility of processing cobalt-chrome parts by additive manufacturing opens the way to the production of complex net-shaped parts able to withstand high loads and at the same time resist to corrosive environment or thermal loads. However, the surface morphology typical of additive manufactured parts could be detrimental to the fatigue life, and finishing operations become necessary. In this paper, the adoption of abrasive fluidized bed (AFB) processing as finishing solution is explored, and the effect of the main process parameters, namely the abrasive type, treatment time and the rotational speed, on morphological features and fatigue life is investigated. Field emission gun-scanning electron microscopy and contact gauge profilometry showed an improvement in sample finishing due to the AFB process. The advantages in surface properties were related to an increase in fatigue life. The experimental results show that AFB has a beneficial effect both on finishing and fatigue behavior of additively manufactured components, a smoother surface was obtained and the crack initiation during fatigue tests was retarded.
Stable and efficient expression of brain-derived neurotrophic factor in tobacco chloroplasts
Authors: Wang Yunpeng , Xu Nuo , Fan Jieying , Wei Zhengyi , Xin Wen , xing shaochen 
Published: June 2022

Abstract
Brain-derived neurotrophic factor (BDNF) is a factor with many functions such as acceleration of cell proliferation and differentiation and is therefore widely used in clinical applications. In this study, an expression vector named pWYP23402 having a codon-optimized BDNF gene was constructed and transferred into chloroplasts of tobacco by gene-gun. After three or four rounds of selection with proper spectinomycin, BDNF was integrated into the chloroplast genome of homoplastomic plants confirmed by PCR and Southern hybridization. ELISA assay indicated that BDNF fused with GFP represented approximately 15.72%±0.33% of total soluble protein in the leaves of transplastomic plants. Moreover, the chloroplast-derived BDNF displayed similar biological activity to the commercial product. This is the first case report of BDNF expression by chloroplast transformation in this model plant, supplying an additional pathway for the production of chloroplast-expressed therapeutic proteins.
Elevated eosinophils as predictor of immune-related adverse events after ipilimumab and nivolumab treatment of advanced and metastatic renal cell carcinoma
Authors: Tasaki Yoshihiko , Hamamoto Shuzo , Sugiyama Yosuke , Tomiyama Nami , Naiki Taku , Etani Toshiki , Taguchi Kazumi , Iwatsuki Shoichiro , Matsuyama Nayuka , Sue Yasuhito , Mimura Yoshihisa , Odagiri Kunihiro , Kubota Hiroki , Noda Yusuke , Hashimoto Yoshihiro , Sakakura Tsuyoshi , Aoki Maria , Ikegami Yosuke , Kobayakawa Yuki , Moritoki Yosinobu , Nozaki Satoshi , Takeda Tomoki , Kurokawa Satoshi , Okada Atsushi , Kawai Noriyasu , Yasui Takahiro , Kimura Kazunori 
Published: June 2022

Abstract
Ipilimumab and nivolumab treatment against advanced and metastatic renal cell carcinoma (RCC) cause severe and lethal immune-related adverse events (irAEs). Predicting irAEs might improve clinical outcomes, however no practical biomarkers exist. This study examined whether eosinophils could be effective biomarkers for irAEs in RCC. We retrospectively analyzed 75 patients with RCC treated with ipilimumab and nivolumab between August 2018 and March 2021 in a multicenter study. The median overall and progression-free survival of patients who experienced irAEs (irAE group) were longer than those of the non-irAE group. Grade ≧2 irAEs were associated with poor mPFS. The eosinophil level two weeks after treatment was significantly elevated in the irAEs compared to non-irAE group (mean, 3.0% vs. 5.7%; P < 0.05). The receiver operating characteristic curve revealed the optimal cutoff value for eosinophil levels against ≧grade 2 irAEs two weeks after treatment was 3.0% (area under the curve=0.699). In multivariate analyses, an eosinophil level ≧3.0% was a risk factor for ≧grade 2 irAEs (odds ratio 4.18, 95% confidence interval 1.16–15.1). An increased eosinophil level two weeks after treatment might be an effective biomarker for ≧grade 2 irAEs in patients with RCC treated with ipilimumab and nivolumab.