bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-biased proteomic response to tomato spotted wilt virus infection of the salivary glands of Frankliniella occidentalis, the western flower thrips
Authors: Rajarapu Swapna Priya , Ben-Mahmoud Sulley , Benoit Joshua B. , Ullman Diane E. , Whitfield Anna E. , Rotenberg Dorith 
Published: July 2022

ABSTRACTSuccessful transmission of tomato spotted wilt virus (TSWV) by Frankliniella occidentalis requires robust infection of the salivary glands (SGs) and virus delivery to plants during salivation. Feeding behavior and transmission efficiency are sexually-dimorphic traits of this thrips vector species. Proteins secreted from male and female SG tissues, and the effect of TSWV infection on the thrips SG proteome are unknown. To begin to discern thrips factors that facilitate virus infection of SGs and transmission by F. occidentalis, we used gel- and label-free quantitative and qualitative proteomics to address two hypotheses: (i) TSWV infection modifies the composition and/or abundance of SG-expressed proteins in adults; and (ii) TSWV has a differential effect on the male and female SG proteome and secreted saliva. Our study revealed a sex-biased SG proteome for F. occidentalis, and TSWV infection modulated the SG proteome in a sex-dependent manner as evident by the number, differential abundance, identities and generalized roles of the proteins. Male SGs exhibited a larger proteomic response to the virus than female SGs. Intracellular processes modulated by TSWV in males indicated perturbation of SG cytoskeletal networks and cell-cell interactions (basement membrane, BM and extracellular matrix proteins, ECM), and subcellular processes consistent with a metabolic slow-down under infection. Several differentially-abundant proteins in infected male SGs play critical roles in viral life cycles of other host-virus pathosystems. In females, TSWV modulated processes consistent with tissue integrity and active translational and transcriptional regulation. A core set of proteins known for their roles in plant cell-wall degradation and protein metabolism were identified in saliva of both sexes, regardless of virus infection status. Saliva proteins secreted by TSWV- infected adults indicated energy generation, consumption and protein turnover, with an enrichment of cytoskeletal/BM/ECM proteins and tricarboxylic acid cycle proteins in male and female saliva, respectively. The nonstructural TSWV protein NSs - a multifunctional viral effector protein reported to target plant defenses against TSWV and thrips - was identified in female saliva. This study represents the first description of the SG proteome and secretome of a thysanopteran and provides many candidate proteins to further unravel the complex interplay between the virus, insect vector, and plant host.
TWISTED DWARF1 mediates myosin XI-associated vesicle trafficking required for auxin transport
Authors: Liu Jie , Zhu Jinsheng , Di Donato Martin , Hao Pengchao , Ren Haiyun , Geisler Markus 
Published: July 2022

ABSTRACTDefects in plant development caused by loss-of the FKBP42, TWISTED DWARF1 (TWD1), have so far been accounted to a dual function of TWD1 acting as an ABCB chaperone that positively regulates ABCB biogenesis and transport activity. On the other hand, TWD1 was characterized as a modulator of actin cytoskeleton bundling and dynamics by interaction with ACTIN7, however, currently it is unclear if both events are connected.Here, we show that TWD1 positively regulates pollen tube germination and growth by controlling actin organization. We identify and verify myosin XI-K as TWD1 interacting protein, which is most likely linking the action of TWD1 on the actin cytoskeleton. We provide evidence that myosin XI-K is required for proper auxin exporter trafficking and auxin export. Further, we show that ER-localized TWD1 reshapes the ER network to overlay actin cables similar to mutations of myosin-XI and thus controls cytoplasmic streaming.In summary, our data support a model in that TWD1 functions as an ER–actin adapter proteins involved in myosin-dependent ER motility and cargo trafficking. Our findings provide a molecular explanation for the defects in early ABCB biogenesis in twd1 that are caused by defects in the three-way interaction between the ER, cytosolic myosin-XI and F-actin.
Do socially responsible higher education institutions contribute to sustainable regional growth and innovation?
Authors: Pedro Eugénia de Matos , Leitão João , Alves Helena 
Published: July 2022

Purpose
This study aims to analyse the efficiency of public higher education institutions (HEIs) through teaching and learning (T&L), research and technology (R&T) and social responsibility (SR) activities. It also aims to assess the external factors influencing the efficiency of T&L, R&T and SR, and influence of this efficiency on sustainable regional economic growth and innovation intensity.

Design/methodology/approach
The empirical approach is based on a two-step data envelopment analysis to compare the efficiency of 23 Portuguese public HEIs, using a Tobit regression, to assess the influence of the factors affecting HEI efficiency which in turn affects regional sustainability and innovation.

Findings
The results lead to the following conclusions: HEIs with better SR efficiency are situated in large urban centres; an insular location is positively associated with HEIs’ T&L and SR efficiency; HEIs’ T&L and SR efficiency positively influence regional gross domestic product (GDP); and HEIs’ R&T efficiency positively influences R&D in regional GDP.

Practical implications
This study offers implications in the domain of sustainable regional growth. The study recommends that the policies of HEIs should concentrate on developing activities that meet the needs of the region. It also emphasizes the need to invest in recruitment of qualified lecturers and researchers, and creation of relevant PhD positions. The study also emphasizes the need for government actions to consider the most disadvantaged regions and create infrastructure to attract new companies and people.

Originality/value
This study contributes to the existing literature on the efficiency of HEIs by considering the efficiency of not only T&L and R&T but also SR. It also analyses the influencers of both HEIs’ efficiency and regional development.
Design and locomotion analysis of a close-chain leg-wheel mobile platform
Authors: Fan Xiang-Ming , Ruan Qiang 
Published: July 2022

Purpose
To take the advantages of terrain-adaptive capability of legged platform and fast-moving ability of wheeled platform, this paper aims to design a leg-wheel mobile platform for obstacle surmounting and analyze the feasibility and locomotivity of different moving modes.

Design/methodology/approach
The platform consists of six leg-wheel units. Each of the units has a close-chain mechanical leg and an actuated wheel at the end of the leg. The platform moves with two modes: legged mode and leg-wheel composite mode. The legged mode achieves high mobility driven by crank motors, while the leg-wheel composite mode achieves obstacle-surmounting ability actuated by crank motors and pitch link motors and obtains high efficiency with the hub motors. The gait planning in different modes has been carried out and the obstacle-surmounting capacity has been analyzed.

Findings
Based on the results of kinematic analysis and gait planning of the close-chain leg-wheel platform, the high mobility and efficiency obstacle-surmounting ability are demonstrated with the two movement modes. The feasibility of the design and the performance of the mobile platform is verified with the prototype experiment. The results of this paper show that the platform possesses good obstacle-surmounting capability.

Originality/value
The work presented in this paper is a novel exploration to design a close-chain leg mechanism and with an actuated wheel in series. The close-chain leg mechanism has the advantages of high leg lift and single degree of freedom characteristics, which makes the platform obtain the ability of obstacle-surmounting.
Examining firm self-regulation in the automobile industry: the role of situational factors, firm characteristics and association influence
Authors: Cavazos David E. , Heller Nathan 
Published: July 2022

PurposeThe current study seeks to contribute to current self-regulation research by first exploring the association between the cost of self-regulation and firm self-regulation. The mediating role of association membership and firm slack is additionally explored.Design/methodology/approachLongitudinal analysis of firm-initiated product recalls for 15 manufacturers in the USA automobile industry from 1966 to 2012 has several important findings regarding the motivations for firm self-regulation.FindingsThe influence of industry associations and firm absorbed slack both contribute to firm self-regulation.Originality/valueThe current study begins to address the importance of firm characteristics in predicting self-regulation activities. The bulk of existing research has examined self-regulation at the industry level as an activity performed as a result of the adoption of formalized industry sanctioned standards of practice. This research contributes to such work by examining firm proactivity in the absence of such formal standards.
The role of trade unions in promoting CSR in Bangladesh’s ready-made garments sector: the post-pandemic scenario
Authors: Khan Md. Rahat , Rammal Hussain Gulzar 
Published: July 2022

PurposeThis study investigates the effectiveness of trade unions in preserving and promoting the rights of the worker, and being their voice in ensuring safe working conditions as part of the firms’ CSR activities.Design/methodology/approachData were collected from employees, managers, and owners of ready-made garments firms in Bangladesh. An open-ended survey instrument was used and distributed widely. Analysis of the 200 responses was undertaken using the qualitative narrative technique.FindingsThe findings show that, unlike traditional labor-management relations, in Bangladesh managers tend to have a more favorable attitude towards unions than employees do. The strong political links between the factory owners and the unions’ leadership raise questions about whose interest the unions represent.Practical implicationsThe authors highlight how adopting a CSR agenda can help unions make better representations on behalf of their members. This, in turn, can enhance the workforce’s efficiency and help strengthen the sector to develop processes to help face disruptions like those experienced during the COVID-19 pandemic.Originality/valueThe study is unique in addressing the role of unions in promoting CSR activities in emerging economies, where the formal institutional application remains limited. The study’s findings can help explain some of the sector’s challenges.
Architectural design research through reflection: a sub-approach under “research by design”
Authors: Uzunkaya Aslı , Paker Kahvecioğlu Nurbin 
Published: July 2022

PurposeThis study is based on a research approach proposal aiming to reveal tacit knowledge that shapes architectural design processes through subjective accumulations and tools. With the premise that design embodies tacit and personal knowledge, it proposes an alternative way to decipher this subjective medium.Design/methodology/approachThe proposed research approach, “(architectural) design research through reflection”, basically belongs to “research by design” method and narrowed in the focus of reflection. It enables to study how tacit knowledge functions within processes of architectural design without being its very subject. The proposal and the product of the approach, revealing diagram, are developed through the “architect's” tools and the involvement of the “researchers” in the process. It is also supported by a conceptual basis created from literature on reflection.FindingsBy means of the proposal, the reflective accumulation of the subjects, that is, the tacit knowledge, is investigated in relation to practice. The revealing diagram is presented as a tool through which relations can be interpreted within the framework of subjects. It is a tool by which the subjects, tools and processes of the architectural design product can be analysed.Originality/valueThe study contributes to architectural research by shifting the perspective on reflective knowledge that shapes design processes.
Antibacterial activity and cytotoxicity of a novel bacteriocin isolated from Pseudomonas sp. strain 166
Authors: Wang Yu , Haqmal M. Aman , Liang Yue‐dong , Muhammad Inam , Zhao Xiao‐Ou , Elken Emad Mohammed , Gao Yun‐Hang , Jia Yu , He Cheng‐guang , Wang Yi‐Ming , Kong Ling‐Cong , Ma Hong‐Xia 
Published: July 2022

SummaryPseudomonas sp. strain 166 was isolated from soil samples from Changbai Mountains. A novel bacteriocin PA166 from Pseudomonas sp. 166 was purified using ammonium sulfate, dextran gel chromatography column and Q‐Sepharose column chromatography successively. The molecular mass of bacteriocin PA166 was found to be 49.38 kDa by SDS‐PAGE and liquid chromatography–mass spectrometry (MS)/MS. Bacteriocin PA166 showed stability at a wide range of pH (2–10), and thermal stability (40, 60, 80 and 100°C). The bacteriocin PA166 antimicrobial activity was slightly inhibited by Ca2+, K+ and Mg2+. The minimum bactericidal concentrations of bacteriocin PA166 against five Pasteurella multocida strains ranged from 2 to 8 μg ml−1. Bacteriocin PA166 showed low cytotoxicity and a higher treatment index (TI = 82.51). Fluorescence spectroscopy indicated that bacteriocin PA166 destroyed the cell membrane to exert antimicrobial activity. In summary, bacteriocin PA166 had strong antibacterial activity, high TI and low toxicity, and hence could serve as a potential clinical therapeutic drug.
Emotion regulation in substance use disorders: a systematic review and meta‐analysis
Authors: Stellern Jordan , Xiao Ke Bin , Grennell Erin , Sanches Marcos , Gowin Joshua L. , Sloan Matthew E. 
Published: July 2022

AbstractBackground and aimsThe ability to regulate emotions effectively has been associated with resilience to psychopathology. Individuals with substance use disorders (SUDs) have been shown to have higher levels of negative emotionality, with some evidence suggesting impairment in emotion regulation compared with individuals without SUDs. However, no previous attempt has been made to systematically review the literature to assess the magnitude of this difference. We aimed to assess the association between SUD diagnosis and emotion regulation as measured by the Difficulties in Emotion Regulation Scale (DERS) and Emotion Regulation Questionnaire (ERQ) through a systematic review and meta‐analysis of existing findings.MethodsThe systematic review was conducted using PubMed, PsycINFO and Embase. We examined cross‐sectional studies that compared a SUD group with a control group and measured emotion regulation using the DERS or the ERQ. The primary analysis focused on papers using the DERS, as this was the predominant instrument in the literature.ResultsTwenty‐two studies met our primary analysis criteria, representing 1936 individuals with a SUD and 1567 controls. Individuals with SUDs relative to controls had significantly greater DERS scores, with a mean difference of 21.44 [95% confidence interval (CI) = 16.49–26.40, P < 0.001] and Hedges’ g = 1.05 (95% CI = 0.86–1.24, P < 0.001). The difference was robust, remaining significant after removing outliers and studies with high risk of bias. Individuals with SUDs demonstrated poorer emotion regulation on each subscale of the DERS, with the largest deficits in the Strategies and Impulse subscales. The ERQ analysis revealed greater use of expressive suppression in those with SUDs relative to controls (Hedges’ g = 0.76, 95% CI = 0.25–1.28, P = 0.004).ConclusionsPeople with substance use disorders appear to have greater difficulties in emotion regulation than people without substance use disorders.
An optimized workflow for microCT imaging of formalin‐fixed and paraffin‐embedded (FFPE) early equine embryos
Authors: Handschuh Stephan , Okada Carolina T. C. , Walter Ingrid , Aurich Christine , Glösmann Martin 
Published: July 2022

AbstractHere, we describe a workflow for high‐detail microCT imaging of formalin‐fixed and paraffin‐embedded (FFPE) equine embryos recovered on Day 34 of pregnancy (E34), a period just before placenta formation. The presented imaging methods are suitable for large animals' embryos with intention to study morphological and developmental aspects, but more generally can be adopted for all kinds of FFPE tissue specimens. Microscopic 3D imaging techniques such as microCT are important tools for detecting and studying normal embryogenesis and developmental disorders. To date, microCT imaging of vertebrate embryos was mostly done on embryos that have been stained with an X‐ray dense contrast agent. Here, we describe an alternative imaging procedure that allows to visualize embryo morphology and organ development in unstained FFPE embryos. Two aspects are critical for high‐quality data acquisition: (i) a proper sample mounting leaving as little as possible paraffin around the sample and (ii) an image filtering pipeline that improves signal‐to‐noise ratio in these inherently low‐contrast data sets. The presented workflow allows overview imaging of the whole embryo proper and can be used for determination of organ volumes and development. Furthermore, we show that high‐resolution interior tomographies can provide virtual histology information from selected regions of interest. In addition, we demonstrate that microCT scanned embryos remain intact during the scanning procedure allowing for a subsequent investigation by routine histology and/or immunohistochemistry. This makes the presented workflow applicable also to archival paraffin‐embedded material.
Patterns of curative treatment for non‐small cell lung cancer in New South Wales, Australia
Authors: Batumalai Vikneswary , Descallar Joseph , Gabriel Gabriel , Delaney Geoff P. , Oar Andrew , Barton Michael B. , Vinod Shalini K. 
Published: July 2022

AbstractIntroductionThere is a lack of large population‐based studies examining patterns of curative treatment for non‐small cell lung cancer (NSCLC) in Australia. This study aimed to evaluate the utilization of curative treatment for NCSLC at a population level and identify factors associated with its use in New South Wales (NSW), Australia.MethodsPatients diagnosed with localized or locoregional NSCLC between 2009 and 2014 were identified from the NSW Central Cancer Registry. Curative treatment was defined as surgery or radiotherapy with a 45 Gy minimum dose. Univariate and multivariable analyses were performed to investigate factors associated with the receipt of curative treatment. A Cox proportional‐hazards regression model was used to analyze the factors associated with 2‐year overall survival (OS).ResultsOf the 5722 patients diagnosed with NSCLC in the study period, 3355 (59%) patients received curative treatment and 2367 (41%) patients did not receive curative treatment. The receipt of curative treatment was significantly associated with younger patients, female gender, localized disease, and Charlson Comorbidity Index (CCI) = 0. The use of curative treatment increased significantly over time from 2009 (55%) to 2014 (63%) and varied significantly from 24% to 70% between local health districts (LHDs) of residence. Younger age, female gender, localized disease, CCI = 0, and overseas country of birth were significantly associated with 2‐year OS. The 2‐year OS significantly improved from 70% in 2009 to 77% in 2014 for patients who received curative treatment.ConclusionThe use of curative treatment for patients with potentially curable NSCLC was low at 59%. However, the use of curative treatment and survival have increased over time. Significant variation was noted in the use of curative treatment between LHDs.
Infection after ureteroscopy for ureteric stones: analysis of 71 305 cases in the Hospital Episode Statistics database
Authors: Veeratterapillay Rajan , Gravestock Paul , Harding Chris , Shaw Matthew , Fitzpatrick John , Keltie Kim , Cognigni Paola , Sims Andrew , Rogers Alistair 
Published: July 2022

ObjectivesTo investigate the burden of infectious complications following ureteroscopy (URS) for ureteric stones on a national level in England using data from the Hospital Episodes Statistics (HES) data warehouse.Materials and MethodsA retrospective cohort was identified and followed up in HES during the period April 2013 to March 2020 for all procedure codes relating to ureteroscopic stone treatment (M27.1, M27.2, M27.3). Treatment episodes relating to the first URS (‘index ureteroscopy’) for each patient were further analysed. All subsequent admissions within 30 days were also captured. The primary outcome was diagnosis of urinary tract infection (UTI; including all codes relating to a UTI/sepsis within the first 30 days of index URS). Secondary outcomes were critical care attendance, attendance at the accident and emergency department (A&E) within 30 days, and mortality.ResultsA total of 71 305 index ureteroscopies were eligible for analysis. The median age was 55 years, and 81% of procedures were elective and 45% were undertaken as day‐cases. At the time of index URS, 16% of patients had diabetes, 0.5% had coexisting neurological disease and 40% had an existing stent/nephrostomy. Overall, 6.8% of the cohort (n = 4822) had a diagnosis of UTI within 30 days of index URS (3.9% immediately after surgery). A total of 339 patients (0.5%) required an unplanned stay in critical care during their index URS admission; 8833 patients (12%) attended A&E within 30 days. Overall mortality was 0.18% (60 in‐hospital, 65 within 30 days); 40 deaths (0.056%) included infection as a contributing cause of death.ConclusionWe present the largest series evaluating infectious complications after ureteroscopic stone treatment. The procedure is safe, with low inpatient infective complication and critical care admission rates.
Micro‐environment alterations through time leading to myeloid malignancies
Authors: Nyamondo Kudzai , Wheadon Helen 
Published: July 2022

The micro‐environment plays a critical role in haematopoietic stem cell (HSC) development, self‐renewal, differentiation and maintenance by providing a supportive cellular framework and essential molecular cues to sustain homeostasis. In ageing and development of age‐related clonal haematopoiesis, the combined contribution of intrinsic alterations in haematopoietic stem cells and their surrounding micro‐environment can promote myeloid skewing and release of pro‐inflammatory cytokines. A pro‐inflammatory micro‐environment is a common feature in the initiation and sustenance of several myeloid malignancies. Furthermore, remodelling of the micro‐environment is recognized to potentiate the survival of malignant over normal cells. This review explores micro‐environmental interactions in the haematopoietic system of adults, especially how the bone marrow micro‐environment is impacted by ageing, the onset of age‐related clonal haematopoiesis and the development of myeloid malignancies. In addition, we also discuss the possible role age‐related clonal haematopoiesis and chronic inflammatory conditions play in altering the bone marrow micro‐environment dynamics. Finally, we explore the importance of in vitro models that accurately mimic different aspects of the bone marrow micro‐environment in order to study normal and malignant haematopoiesis.LINKED ARTICLESThis article is part of a themed issue on Cancer Microenvironment and Pharmacological Interventions. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v181.2/issuetoc