bluuebunny/crossref_metadata_2025_split huggingface dataset:
AWARE. A web application to rapidly assess cardiovascular risk in Type 2 Diabetes Mellitus.
Authors: Berra Cesare Celeste , Manfrini Roberto , Mirani Marco , Bucciarelli Loredana , Zakaria Amhed S , Piccini Sara , Ghelardi Renata , Lunati Maria Elena , Rodovalho Sylka , Bifari Francesco , Fiorina Paolo , Folli Franco 
Published: October 2022

Abstract
Background
Cardiovascular (CV) risk assessment may result unpractical in real-world clinical practice, although being considered a key step for choosing appropriate therapies for patients with Type 2 Diabetes Mellitus (T2DM). In order to streamline this process in the diabetes clinic, we have developed the web application “AWARE”.
Methods
The AWARE App is based on 2019 ESC/EASD criteria for cardiovascular risk (CVR) stratification in T2DM, which divides patients into 3 categories: very high (VHCVR), high (HCVR) and moderate (MCVR) CV risk. In this retrospective clinical study, we employed the AWARE App to assess CV risk of consecutive T2DM patients attending Diabetes Clinics in Lombardy (Italy).
Results
Overall, 2243 T2DM patients underwent CV risk assessment with the AWARE App. 1619 patients (72.2%) had a VHCVR, 199 (8.9%) an HCVR, and only 17 (0.8%) had an MCVR. 408 patients (18.2%) did not fit into any of the ESC/EASD risk categories and we included them in the additional “moderate-to-high” (MHCVR) group. Patients with VHCVD were more frequently ≥ 65 years old (68.9%), with a longer disease duration (≥ 10 years [56.8%]), history of CV disease (41.4%), organ damage (35.5%) and higher numbers of CV risk factors compared with other risk groups. Patients with MHCVD generally had disease duration < 10 years (96%), younger age (50–60 years [55%]), no history of CV disease and no organ damage, and 1–2 CV risk factors (89%). GLP-1 RA or SGLT-2i were prescribed only to 26.3% of the patients with VHCVR and to 24.7% of those with HCVR. Glycaemic control was unsatisfactory, both in the overall population and in each CV risk group (mean A1c level of 58.7 ± 13.44 mmol/mol [7.5 ± 3.4%]).
Conclusions
The AWARE App is a practical tool for CV risk stratification of T2DM patients in real-world clinical practice. Despite a generally severe CV risk and unsatisfactory glycaemic control, T2DM patients are rarely treated to achieve HbA1c < 7% and with newer cardioprotective medications.
Tetramethylpyrazine protects mitochondrial function by upregulating TFAM and alleviating nerve cell apoptosis in SBI rats
Authors: Wang Chaoyu , Huang Yaqian , Gong Yating , Wu Muyao , Jiang Lei , Xu Jianguo , Dang Baoqi , Chen Gang 
Published: October 2022

Abstract
Mitochondrial dysfunction caused by mitochondrial DNA (mtDNA) damage and mutation is widely accepted as one of the pathological processes of neurodegenerative diseases. As an mtDNA binding protein, mitochondrial transcription factor A (TFAM) maintains the integrity of mtDNA through transcription, replication, nucleoid formation, damage perception, and DNA repair. In recent works, the overexpression of TFAM increased the mtDNA copy count, promoted mitochondrial function, and improved the neurological dysfunction of neurodegenerative diseases. The role of TFAM in neurodegenerative diseases has been well explained. However, the role of TFAM after surgical brain injury (SBI) has not been studied. In this work, we aimed to study the role of TFAM in the brain after SBI and its mechanism of action. One hour after the occurrence of SBI, tetramethylpyrazine (TMP) was injected into the abdominal cavity of rats, and the brain was collected 48 hours later for testing. The evaluation included neurobehavioral function test, brain water content measurement, immunofluorescence, western blot, TUNEL staining, FJC staining, ROS test, and ATP test. The results showed that after SBI, the content of TFAM on the ipsilateral side increased and reached a peak at about 48 h. After intraperitoneal injection of TMP in rats, 48 hours after SBI, the concentration of TFAM, Bcl-2, and ATP increased; the content of caspase-3, ROS, and cerebral edema decreased; and the nerve function significantly improved. We conclude that TMP inhibited cell apoptosis after surgical brain injury in rats by upregulating TFAM and protecting brain tissues.
Study on evolution law of bearing stress of coal pillar in steeply inclined coal seam section of mountain topography
Authors: Tang Long , Tu Shihao , Tu Hongsheng , Liu Xun , Miao Kaijun , Li Wenlong , Zhao Hongbin , Ma Jieyang , Li Yan 
Published: October 2022

Abstract
The stability of section coal pillar in steeply inclined coal seam under mountain landform is beneficial to the stability of surface and mountain. Based on the engineering background of N2821 working face in Fengchun coal mine, the force model of the steeply inclined coal pillar under the mountain and the three-dimensional numerical model of the landform characteristics of the mountain are established; the necessary conditions and reasonable size calculation of the steeply inclined coal pillar under the mountain are analyzed; the bearing stress evolution law of the steeply inclined coal pillar under the mountain is studied. The research shows that when the dip angle of coal seam is greater than the arctangent value of the sum of friction coefficients of upper and lower surfaces of coal pillar, the coal pillar has sliding instability ; the reasonable coal pillar size of N2821 working face is 12 m. After working face mining, due to the short size of coal pillar, there is no obvious elastic core area ; with the advancement of the working face, the coal pillar in the middle of the goaf is most affected by the stress superposition, and the shear stress of the coal and rock mass gradually increases and concentrates on the coal pillar ; although the coal pillar is plastically damaged by mining, it still has good supporting capacity. The field monitoring data analysis is consistent with the simulation conclusion, and the coal pillar size is reasonable. The research results have certain reference and reference significance for coal pillar retention under similar conditions.
Free Entry into a Co-opetitive Mixed Oligopoly
Authors: Yoshikawa Takeshi 
Published: October 2022

Abstract
We investigate the socially desirable number of entrants in a free-entry co-opetitive mixed market. Our study simulates a public firm and private firm engaging in Cournot competition with a homogeneous good while sharing common property resources that affect industry-wide demand. To this end, we introduce a strategic commitment competition stage between market entry and production, where firms pursue demand-enhancing investments. We find that the relationship between the number of firms entering and the profit of incumbent private firms is nonmonotonic (inverted U-shape) when the investment cost parameter is sufficiently small. Moreover, free private firm entry can lead to either socially excessive or insufficient entry.
JEL Code: C72, L13, L33
Comprehensive Genomic Profiling Reveals Molecular Subsets of ASXL1-Mutated Myeloid Neoplasms
Authors: Johnson Steven , Haberberger James , Ramkissoon Lori , Galeotti Jonathan , Coombs Catherine , Richardson Daniel , Foster Matthew , Duncan Daniel , Zeidner Joshua , Ferguson Naomi , Montgomery Nathan 
Published: October 2022

Abstract
ASXL1 is a dynamic epigenetic regulator frequently mutated in myeloid neoplasia. However, a large-scale analysis of patients with ASXL1-mutated myeloid disease has not been performed to date. We retrospectively analyzed comprehensive genomic profiling results from clinical samples from 6,043 adult patients to characterize the spectrum of ASXL1 mutation sites, co-mutation patterns, and to investigate for clinicopathologic differences between ASXL1-wild type patients. ASXL1 mutations occurred in 1,414 patients (23%). Patients with the hotspot c.1934dupG mutation had phenotypic and co-mutational distinctions from those with other ASXL1 mutations. Notably, TP53 (p < 0.01) and SETBP1 (p = 0.04) mutations more often occurred with non-c.1934dupG mutations in acute myeloid leukemia (AML). ASXL1 cohesin-binding motif (CBM) mutations were generally, but not mutually exclusive with cohesin mutations. Co-mutation patterns further suggested that non-cohesin driver mutations may be selectively favored when the CBM is compromised. Overall, ASXL1-mutated and ASXL1-wild type patients had significant differences in age, sex, ancestry, and co-mutation burden (p < 0.01). Mutation co-occurrence and mutual exclusivity testing revealed strong co-occurrence (q < 0.01) between mutations in ASXL1 and nine genes (SRSF2, U2AF1, RUNX1, SETBP1, EZH2, STAG2, CUX1, CSF3R, CBL), and we further analyzed patients with these co-mutations. We found that in spliceosome co-mutated patients, mutational characteristics suggested that ASXL1/SF3B1 co-mutation may be distinct from ASXL1/non-SF3B1 spliceosome co-mutation. In ASXL1/SRSF2 co-mutated AML, STAG2 (42%) and SETBP1 (16%) mutations were common and dependent on the presence of both ASXL1 and SRSF2 mutations (p < 0.05). STAG2 and SETBP1 mutations were mutually exclusive in ASXL1/SRSF2 co-mutated patients and were associated with divergent phenotypes in chronic myeloid neoplasms. Our findings in a large cohort support that ASXL1 mutation site and certain ASXL1 multi-mutant genotypes may be biologically relevant in patients with myeloid neoplasia, and further study is warranted to assess for therapeutic or prognostic impacts in these molecular subsets.
Concentration of biogenic and risk elements in wild boar testes and their interactions with sperm quality
Authors: Halo Marko , Solár Dušan , Tirpák Filip , Miškeje Michal , Albrycht Marzena , Massányi Martin , Dianová Lucia , Zemanová Jiřina , Binkowski Lukasz J. , Stawarz Robert , Massányi Peter 
Published: October 2022

Abstract
The purpose of this study was to monitor concentration of selected biological and risk elements in testes and later epididymal spermatozoa motility of wild boars (Sus scrofa scrofa) as well as their association. Wild boars were hunted in region Žuhračka - Levice branch plant, Slovak Republic. Testes were surgically removed post-mortem and were transported to the laboratory. Spermatozoa were obtained by dissecting the distal portion of the cauda epididymis and were analysed by Computer assisted semen analyzer (CASA) system. Concentration of elements were measured by inductively-coupled plasma optical emission spectrometry (ICP-OES) and by cold vapor absorption spectrometer (MA-3 Solo Mercury Analyzer). Total spermatozoa motility was at the level of 44.29% and progressive motility was 18.47%. Concentration of elements in testes was in following order: K > Na > Mg > Ca > Fe > Zn > Al > Cu > Se > Mn > As > Cr > Pb > Mo > Sr > Ni > Ba > Cd > Li > Hg. Negative association was observed between Se and motility, progressive motility, further between As and velocity curved line and beat cross frequency. Mercury showed positive correlation with beat cross frequency. Our results suggest that some chemical elements accumulated from polluted environment can affect reproduction of wild animals.
Trace metals in the Arabian seawater and freshwater samples in Oman by using by ICP-OES
Authors: Alsaid Ayman Alsaid Ibrahim , Ahmed Aya Mohammed Amin , Qamshouai Rayyan Hamed Al , Akhtar Mohammad Sohail , Touby Salem Said Jaroof Al , Hossain Md Amzad 
Published: October 2022

Abstract
Water is an inorganic, transparent, tasteless, odorless, colorless liquid which is the main component of the earth and living organisms. Trace metals are elements that usually occur at very low levels in the environment. These metals are needed for life. However, at high concentrations of the trace metals can be toxic. The contamination with trace metals in water samples are health hazards and it can create many health problems such as liver, kidney, and intestinal damage, anaemia, and cancer. Therefore, the aim of this work was to assess the levels of lead (Pb), arsenic (As), nickel (Ni), chromium (Cr), and cadmium (Cd) in the sea and freshwater samples, which was collected from five major seaport areas in Oman and one fresh water canal by using sensitive Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). To achieve these objectives, sixty-four water samples were collected from six different locations in Oman. A total sixty seawater samples were collected from Matrah port, Al Mouj Marina, Sohar port, Salalah port, and Al Duqm port and four freshwater samples were collected from Falaj Daris. The conductivity of the collected seawater samples were very high therefore, the seawater samples were diluted 200 times and analyzed by ICP-OES. The results obtained from ICP-OES showed that the level of all analyzed water samples, the concentrations of trace metals (As, Ni, Cd, and Cr) in sea and freshwater samples were within the permissible limit except the concentration of Pb, which exceeded the permissible limit in several samples. The toxic Pb contaminated water samples can create a health risk therefore appropriate water treatment should be applied to reduce the concentration of Pb within the safe limit so the accumulation of toxic waste in both humans and animals can be avoided. So far of our knowledge, the ICP-OES method is the first analytical technique for the detection of toxic heavy metals in water samples. In conclusion, the developed method could be used routinely for the detection of heavy metals in water samples.
Characterization of 940 Chinese patients with triple-positive breast cancer by clinicopathological and treatment outcomes
Authors: Liang Ying , Jia Yongsheng , Zhao Weipeng , Tong Zhongsheng , Shi Yehui 
Published: October 2022

Abstract
Background
This study aims to evaluate the clinicopathological features, prognosis, and related prognosis factors of triple-positive breast cancer, to develop more scientific and individualized treatment plans.
Methods
We collected pathological and clinical data from 960 patients with early-stage triple-positive breast cancer who underwent surgical treatment at Tianjin Medical University Cancer Institute and Hospital (2012–2017). The Cox regression model was used for prognostic univariate analysis and multifactor analysis. Kaplan-Meier was used to plot survival curves, and the log-rank test was used to analyze survival differences between groups.
Results
T-stage, N-stage, whether to receive adjuvant targeted therapy, and whether to receive adjuvant endocrine therapy were independent influencing factors of prognosis (P < 0.05). Subgroup analysis showed that sequential tamoxifen treatment alone in the targeted therapy group did not significantly improve the prognosis of patients (P < 0.05). The benefit of endocrine treatment was not significant in low ER-positive breast cancer (P > 0.05). The prognosis of T1abN0M0 patients was not significantly altered by the use of trastuzumab or not (P = 0.439). There was no significant difference in OS with or without trastuzumab in the HR ≥ 30% group (P = 0.212) and in DFS and OS in the HR ≥ 50% group (P = 0.082, P = 0.978).
Conclusions
Our findings indicate that HR expression influences the biological behavior and treatment outcome of TPBC. We should choose individualized, targeted treatment programs, based on patients’ HR expression and pathological staging to benefit patients with TPBC.
“A glimmer of hope” - Perceptions, barriers, and drivers for medicinal cannabis use amongst Australian and New Zealand people with endometriosis: A qualitative study.
Authors: Sinclair Justin , Abbott Jason , Mikocka-Walus Antonina , Ng Cecila , Sarris Jerome , Armour Mike 
Published: October 2022

Abstract
Previous quantitative research has shown that cannabis use, mostly illicit, is used for symptom management amongst those with endometriosis living in Australia or New Zealand, but the drivers and barriers for use of legal, medicinal cannabis in this population are currently unclear. This study sought to investigate, via online focus-groups, the perceptions, barriers, drivers, and experiences associated with cannabis use, whether legal or illicit, amongst 37 Australians and New Zealanders, aged 18–55, with a medical diagnosis of endometriosis. Previous cannabis usage was not required to participate. Discussion topics included strategies employed to manage symptoms, exploration of current medications, previous use of cannabis for pain management, and interest in using medicinal cannabis as a management strategy. Barriers to medicinal cannabis were also explored. Audio was recorded, transcribed, and analyzed using a content analysis approach. Of the 37 participants, 73% had used cannabis previously, either recreationally, for symptom management or both. Participants with moderate to severe symptoms of medically diagnosed endometriosis reported inadequacies with their current medical and self-management strategies and were inclined to try medicinal cannabis, both as part of their medical management and as part of a clinical trial. Barriers to medicinal cannabis adoption identified in this cohort included high costs of legal cannabis products, lack of clarity and fairness in current roadside drug testing laws and workplace drug testing policies, concern over the impact of stigma affecting familial, social and workplace life domains, and subsequent judgement and the lack of education/engagement from their medical providers regarding cannabis use. Given the interest in medicinal cannabis and the reported lack of effective symptom management, clinical trials are urgently required to determine the potential role that medicinal cannabis may play in reducing the symptoms of endometriosis.
Preparation of Nanofluid of Lanthanum Borate Nanosheets and Investigation of Its Tribological Properties and Tribomechamisms in Different Base Oils
Authors: Wu Jing , Yang Guangbin , Zhang Shengmao , Zhang Yujuan , Sun Lu , Sun Tianhua , Yu Laigui , Zhang Pingyu 
Published: October 2022

Abstract
Oleic acid-modified lanthanum borate nanosheets (OA-LBNs) were prepared by simple surface modification technology combined with precipitation method. The structure of the as-prepared light yellow transparent nanofluid of OA-LBNs was analyzed by Fourier transform infrared spectroscopy and transmission electron microscopy, and its thermal stability was evaluated by thermogravimetric analysis. Moreover, the tribological properties of OA-LBNs nanofluid as the lubricant additive in diisooctylsebacate (DIOS), poly-α-olefin (PAO4), and rapeseed oil (RO) were evaluated with a four-ball friction and wear tester, and its tribomechanisms in the three kinds of base oils were also discussed. The results show that OA-LBNs nanofluid exhibits good antiwear ability in the three kinds of base oils; and in particular, OA-LBNs nanofluid added in PAO4 shows the best antiwear ability. The tribological properties of the OA-LBNs nanofluid as the lubricant additive are dependent on the nature of the base oils. Namely, the polarity of the base oil influences the adsorption of the OA-LBNs nanofluid on the rubbed steel surface, thereby affecting the composition of the tribofilm formed on the rubbed steel surface and resulting in changes in tribological properties.
Persistent headaches one year after bacterial meningitis: prevalence, determinants and impact on quality of life
Authors: Van Rémi Nguyen , Tubiana Sarah , Debroucker Thomas , Cédric Joseph , Brocas Elsa , Roy Carine , Meyohas Marie-Caroline , Prazuck Thierry , Chirouze Catherine , Hoen Bruno , Xavier Duval , Revest Matthieu 
Published: October 2022

Abstract
Background.

Little is known on headaches long-term persistence after bacterial meningitis and on their impact on patients’ quality of life.

Methods.

In an ancillary study of the French national prospective cohort of community-acquired bacterial meningitis in adults (COMBAT) conducted between February 2013 and July 2015, we collected self-reported headaches before, at onset, and 12 months (M12) after meningitis. Determinants of persistent headache (PH) at M12, their association with M12 quality of life (SF 12), depression (Center for Epidemiologic Studies Depression Scale) and neuro-functional disability were analysed.

Results.

Among the 277 alive patients at M12 87/274 (31.8%), 213/271 (78.6%) and 86/277 (31.0%) reported headaches before, at the onset, and at M12, respectively. In multivariate analysis, female sex (OR: 2.75 [1.54–4.90]; p < 0.001), pre-existing headaches before meningitis (OR: 2.38 [1.32–4.30]; p < 0.01), higher neutrophilic polynuclei percentage in the CSF of the initial lumbar puncture (OR: 1.02 [1.00-1.04]; p < 0.05), and brain abscess during the initial hospitalisation (OR: 8.32 [1.97–35.16]; p < 0.01) were associated with M12 persistent headaches. Neither the responsible microorganism, nor the corticoids use were associated with M12 persistent headaches. M12 neuro-functional disability (altered Glasgow Outcome Scale; p < 0.01), M12 physical handicap (altered modified Rankin score; p < 0.001), M12 depressive symptoms (p < 0.0001), and M12 altered physical (p < 0.05) and mental (p < 0.0001) qualities of life were associated with M12 headaches.

Conclusion.

Persistent headaches are frequent one year after meningitis and are associated with quality of life alteration.
Association Of The LH/FSH Ratio With Electrocardiogram Abnormalities In Polycystic Ovarian Syndrome
Authors: Wu Lin , Zheng Shuhui , Zhao Guangyin , Tian Borui , Sun Yucong , Tan Ying , Tang Xixiang , Qian Xiaoxian , Chen Yanming 
Published: October 2022

Abstract
Objective: This study aimed to investigate the association of the Luteinizing/Follicle-stimulating hormone (LH/FSH) ratio with Electro-cardiogram (ECG) features in Polycystic Ovarian Syndrome (PCOS).
Methods: Consecutive PCOS patients’ record were recruited from the database of the hospital for the cross-sectional study. Serum concentrations of testosterone, pituitary, prolactin, progesterone, estradiol, LH, and FSH were measure and 12-lead ECG was performed for each subject. According to LH/FSH ratio, subjects were divided into High Group (LH/FSH Ratio ≥ 2, n=24), and Low Group (LH/FSH Ratio < 2, n=52). 180 ECG features were automatically extracted by an ECG management system. The relationship between LH/FSH ratio and ECG variables was assessed by univariate and multivariate linear regression models.
Results: 76 patients with PCOS were enrolled. The mean values of LH in High Group were significantly higher than that in Low Group (P < 0.0001). P wave changes, QRS duration and ST segment changes had significantly difference between the two groups. The beginning of P wave was lower in Lead I, aVL, V2, V5, V6 in High group. Compared to Low Group, QRS duration, R-Q interval and R-S interval was narrow in High Group. There were ST depression in High Group, especially in left precordial leads such as Lead I, aVL, V5 and V6. In PCOS patients, P wave, QRS duration and ST segment changes were the most abnormalities in ECG of High LH/FSH ratio Group.
Conclusions: The associations between LH/FSH ratio and ECG abnormalities were enhanced by the increased risk stratification of PCOS patients.
Integration and entry decisions of a vertically integrated foreign firm
Authors: Zhang Chuyuan , Lee Sang-Ho 
Published: October 2022

Abstract
This study constructs a vertical structure model in which domestic upstream and downstream firms compete with an integrated foreign firm in each market, and compares the entry decisions of the foreign firm under vertical separation and integration between domestic firms. We show that a foreign firm will switch its entry decisions in both upstream and downstream markets depending on separation and integration, which also affects the supply decision of the integrated domestic firm. We also demonstrate that the entry decision under separation is socially beneficial only when the foreign firm enters the final goods market, while entry under integration is never socially beneficial. Finally, we find that integration decreases welfare when the number of downstream firms’ ex-post integration is sufficiently small or large.
JEL Classification L32 · L52 · D43 · H44