bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quorum sensing and iron-dependent coordinated control of autoinducer-2 production via small RNA RyhB in Vibrio vulnificus
Authors: Lee Keun-Woo , Wen Yancheng , Park Na-Young , Kim Kun-Soo 
Published: January 2022

AbstractRoles for the non-coding small RNA RyhB in quorum-sensing and iron-dependent gene modulation in the human pathogen V. vulnificus were assessed in this study. Both the quorum sensing master regulator SmcR and the Fur-iron complex were observed to bind to the region upstream of the non-coding small RNA RyhB gene to repress expression, which suggests that RyhB is associated with both quorum-sensing and iron-dependent signaling in this pathogen. We found that expression of LuxS, which is responsible for the biosynthesis of autoinducer-2 (AI-2), was higher in wild type than in a ryhB-deletion isotype. RyhB binds directly to the 5′-UTR (untranslated region) of the luxS transcript to form a heteroduplex, which not only stabilizes luxS mRNA but also disrupts the secondary structure that normally obscures the translational start codon and thereby allows translation of LuxS to begin. The binding of RyhB to luxS mRNA requires the chaperone protein Hfq, which stabilizes RyhB. These results demonstrate that the small RNA RyhB is a key element associated with feedback control of AI-2 production, and that it inhibits quorum-sensing signaling in an iron-dependent manner. This study, taken together with previous studies, shows that iron availability and cell density signals are funneled to SmcR and RyhB, and that these regulators coordinate cognate signal pathways that result in the proper balance of protein expression in response to environmental conditions.
Long-term metal fume exposure assessment of workers in a shipbuilding factory
Authors: Wang Ying-Fang , Kuo Yu-Chieh , Wang Lin-Chi 
Published: January 2022

AbstractThis study aims to assess the metal fume exposure of welders and to determine exposure rates for similar exposure groups in a shipyard through the use of Near-field/Far-field (NF/FF) mathematical model and Bayesian decision analysis (BDA) technique. Emission rates of various metal fumes (i.e., total chromium (Cr), iron (Fe), lead (Pb), manganese (Mn), and nickel (Ni)) were experimentally determined for the gas metal arc welding and flux cored arc welding processes, which are commonly used in shipyards. Then the NF/FF field model which used the emission rates were further validated by welding simulation experiment, and together with long-term operation condition data obtained from the investigated shipyard, the predicted long-term exposure concentrations of workers was established and used as the prior distribution in the BDA. Along with the field monitoring metal fume concentrations which served as the likelihood distribution, the posterior decision distributions in the BDA were determined and used to assess workers’ long-term metal exposures. Results show that the predicted exposure concentrations (Cp) and the field worker’s exposure concentrations (Cm) were statistically correlated, and the high R2 (= 0.81–0.94) indicates that the proposed surrogate predicting method by the NF and FF model was adequate for predicting metal fume concentrations. The consistency in both prior and likelihood distributions suggests the resultant posterior would be more feasible to assess workers’ long-term exposures. Welders’ Fe, Mn and Pb exposures were found to exceed their corresponding action levels with a high probability (= 54%), indicating preventive measures should be taken immediately. The proposed approach provides a universal solution for conducting exposure assessment with usual limited number of personal exposure data.
Accuracy of vascular tortuosity measures using computational modelling
Authors: Kashyap Vishesh , Gharleghi Ramtin , Li Darson D. , McGrath-Cadell Lucy , Graham Robert M. , Ellis Chris , Webster Mark , Beier Susann 
Published: January 2022

AbstractSevere coronary tortuosity has previously been linked to low shear stresses at the luminal surface, yet this relationship is not fully understood. Several previous studies considered different tortuosity metrics when exploring its impact of on the wall shear stress (WSS), which has likely contributed to the ambiguous findings in the literature. Here, we aim to analyze different tortuosity metrics to determine a benchmark for the highest correlating metric with low time-averaged WSS (TAWSS). Using Computed Tomography Coronary Angiogram (CTCA) data from 127 patients without coronary artery disease, we applied all previously used tortuosity metrics to the left main coronary artery bifurcation, and to its left anterior descending and left circumflex branches, before modelling their TAWSS using computational fluid dynamics (CFD). The tortuosity measures included tortuosity index, average absolute-curvature, root-mean-squared (RMS) curvature, and average squared-derivative-curvature. Each tortuosity measure was then correlated with the percentage of vessel area that showed a < 0.4 Pa TAWSS, a threshold associated with altered endothelial cell cytoarchitecture and potentially higher disease risk. Our results showed a stronger correlation between curvature-based versus non-curvature-based tortuosity measures and low TAWSS, with the average-absolute-curvature showing the highest coefficient of determination across all left main branches (p < 0.001), followed by the average-squared-derivative-curvature (p = 0.001), and RMS-curvature (p = 0.002). The tortuosity index, the most widely used measure in literature, showed no significant correlation to low TAWSS (p = 0.86). We thus recommend the use of average-absolute-curvature as a tortuosity measure for future studies.
MEG8 regulates Tissue Factor Pathway Inhibitor 2 (TFPI2) expression in the endothelium
Authors: Kremer Veerle , Bink Diewertje I. , Stanicek Laura , van Ingen Eva , Gimbel Theresa , Hilderink Sarah , Günther Stefan , Nossent Anne Yaël , Boon Reinier A. 
Published: January 2022

AbstractA large portion of the genome is transcribed into non-coding RNA, which does not encode protein. Many long non-coding RNAs (lncRNAs) have been shown to be involved in important regulatory processes such as genomic imprinting and chromatin modification. The 14q32 locus contains many non-coding RNAs such as Maternally Expressed Gene 8 (MEG8). We observed an induction of this gene in ischemic heart disease. We investigated the role of MEG8 specifically in endothelial function as well as the underlying mechanism. We hypothesized that MEG8 plays an important role in cardiovascular disease via epigenetic regulation of gene expression. Experiments were performed in human umbilical vein endothelial cells (HUVECs). In vitro silencing of MEG8 resulted in impaired angiogenic sprouting. More specifically, total sprout length was reduced as was proliferation, while migration was unaffected. We performed RNA sequencing to assess changes in gene expression after loss of MEG8. The most profoundly regulated gene, Tissue Factor Pathway Inhibitor 2 (TFPI2), was fivefold increased following MEG8 silencing. TFPI2 has previously been described as an inhibitor of angiogenesis. Mechanistically, MEG8 silencing resulted in a reduction of the inhibitory histone modification H3K27me3 at the TFPI2 promoter. Interestingly, additional silencing of TFPI2 partially restored angiogenic sprouting capacity but did not affect proliferation of MEG8 silenced cells. In conclusion, silencing of MEG8 impairs endothelial function, suggesting a potential beneficial role in maintaining cell viability. Our study highlights the MEG8/TFPI2 axis as potential therapeutic approach to improve angiogenesis following ischemia.
Targeting of non-coding RNAs encoded by novel MYC enhancers inhibits the proliferation of human hepatic carcinoma cells in vitro
Authors: Choi Hae In , An Ga Yeong , Yoo Eunyoung , Baek Mina , Chai Jin Choul , Binas Bert , Lee Young Seek , Jung Kyoung Hwa , Chai Young Gyu 
Published: January 2022

AbstractThe proto-oncogene MYC is important for development and cell growth, however, its abnormal regulation causes cancer. Recent studies identified distinct enhancers of MYC in various cancers, but any MYC enhancer(s) in hepatocellular carcinoma (HCC) remain(s) elusive. By analyzing H3K27ac enrichment and enhancer RNA (eRNA) expression in cultured HCC cells, we identified six putative MYC enhancer regions. Amongst these, two highly active enhancers, located ~ 800 kb downstream of the MYC gene, were identified by qRT-PCR and reporter assays. We functionally confirmed these enhancers by demonstrating a significantly reduced MYC expression and cell proliferation upon CRISPR/Cas9-based deletion and/or antisense oligonucleotide (ASO)-mediated inhibition. In conclusion, we identified potential MYC enhancers of HCC and propose that the associated eRNAs may be suitable targets for HCC treatment.
Development of soil-less substrates capable of degrading organic nitrogen into nitrate as in natural soils
Authors: Meeboon Jamjan , Nishida Ryoya , Iwai Takashi , Fujiwara Kazuki , Takano Masao , Shinohara Makoto 
Published: January 2022

AbstractSoil-less substrates are unable to catalyse nitrification because the addition of a high concentration of organic substances suppresses nitrification. We used a previously developed multiple parallel mineralization method, which enables the use of organic fertilizers in hydroponics, to support nitrification in soil-less substrates. In this method, microorganisms immobilized on porous substrates produced inorganic nitrate from organic substances, as in a natural soil. Phosphate and potassium ions were also released. Microorganisms produced nitrate from organic substances when immobilized on polyurethane resin, rockwool, vermiculite, oyster shell lime, and rice husk charcoal. The optimal amount of organic substance added daily to 100 mL of substrate held 6 mg of organic N. The optimal incubation temperature ranged from 25 to 42 °C. A high relative humidity (51% or higher) was more suitable than drier conditions (20%). The optimal amount of fish fertilizer added to the substrate was 6 mg organic N. The lower the C/N ratio of the organic substance, the better the result. Vegetable plants grew well on inoculated substrates but not on uninoculated substrates. These results show that soil-less substrates can be used to create artificial soils in which plants can be grown with the addition of organic fertilizer, as in a natural soil.
Neuronally-enriched exosomal microRNA-27b mediates acute effects of ibuprofen on reward-related brain activity in healthy adults: a randomized, placebo-controlled, double-blind trial
Authors: Burrows Kaiping , Figueroa-Hall Leandra K. , Kuplicki Rayus , Stewart Jennifer L. , Alarbi Ahlam M. , Ramesh Rajagopal , Savitz Jonathan B. , Teague T. Kent , Risbrough Victoria B. , Paulus Martin P. 
Published: January 2022

AbstractThis double-blind, randomized, within-subjects design evaluated whether acute administration of an anti-inflammatory drug modulates neuron-specific, inflammation-modulating microRNAs linked to macroscopic changes in reward processing. Twenty healthy subjects (10 females, 10 males) underwent a functional magnetic resonance imaging scan while performing a monetary incentive delay (MID) task and provided blood samples after administration of placebo, 200 mg, or 600 mg of ibuprofen. Neuronally-enriched exosomal microRNAs were extracted from serum and sequenced. Results showed that: (1) 600 mg of ibuprofen exhibited higher miR-27b-3p, miR-320b, miR-23b and miR-203a-3p expression than placebo; (2) higher mir-27b-3p was associated with lower insula activation during MID loss anticipation; and (3) there was an inverse relationship between miR-27b-3p and MID gain anticipation in bilateral putamen during placebo, a pattern attenuated by both 200 mg and 600 mg of ibuprofen. These findings are consistent with the hypothesis that miR-27b could be an important messaging molecule that is associated with regulating the processing of positive or negative valenced information.
On electrically tunable stacking domains and ferroelectricity in moiré superlattices
Authors: Bennett Daniel , Remez Benjamin 
Published: January 2022

AbstractIt is well known that stacking domains form in moiré superlattices due to the competition between the interlayer van der Waals forces and intralayer elastic forces, which can be recognized as polar domains due to the local spontaneous polarization in bilayers without centrosymmetry. We propose a theoretical model which captures the effect of an applied electric field on the domain structure. The coupling between the spontaneous polarization and field leads to uneven relaxation of the domains, and a net polarization in the superlattice at nonzero fields, which is sensitive to the moiré period. We show that the dielectric response to the field reduces the stacking energy and leads to softer domains in all bilayers. We then discuss the recent observations of ferroelectricity in the context of our model.
Discovery of signatures of fatal neonatal illness in vital signs using highly comparative time-series analysis
Authors: Niestroy Justin C. , Moorman J. Randall , Levinson Maxwell A. , Manir Sadnan Al , Clark Timothy W. , Fairchild Karen D. , Lake Douglas E. 
Published: January 2022

AbstractTo seek new signatures of illness in heart rate and oxygen saturation vital signs from Neonatal Intensive Care Unit (NICU) patients, we implemented highly comparative time-series analysis to discover features of all-cause mortality in the next 7 days. We collected 0.5 Hz heart rate and oxygen saturation vital signs of infants in the University of Virginia NICU from 2009 to 2019. We applied 4998 algorithmic operations from 11 mathematical families to random daily 10 min segments from 5957 NICU infants, 205 of whom died. We clustered the results and selected a representative from each, and examined multivariable logistic regression models. 3555 operations were usable; 20 cluster medoids held more than 81% of the information, and a multivariable model had AUC 0.83. New algorithms outperformed others: moving threshold, successive increases, surprise, and random walk. We computed provenance of the computations and constructed a software library with links to the data. We conclude that highly comparative time-series analysis revealed new vital sign measures to identify NICU patients at the highest risk of death in the next week.
Heterogeneous digital biomarker integration out-performs patient self-reports in predicting Parkinson’s disease
Authors: Deng Kaiwen , Li Yueming , Zhang Hanrui , Wang Jian , Albin Roger L. , Guan Yuanfang 
Published: January 2022

AbstractParkinson’s disease (PD) is one of the first diseases where digital biomarkers demonstrated excellent performance in differentiating disease from healthy individuals. However, no study has systematically compared and leveraged multiple types of digital biomarkers to predict PD. Particularly, machine learning works on the fine-motor skills of PD are limited. Here, we developed deep learning methods that achieved an AUC (Area Under the receiver operator characteristic Curve) of 0.933 in identifying PD patients on 6418 individuals using 75048 tapping accelerometer and position records. Performance of tapping is superior to gait/rest and voice-based models obtained from the same benchmark population. Assembling the three models achieved a higher AUC of 0.944. Notably, the models not only correlated strongly to, but also performed better than patient self-reported symptom scores in diagnosing PD. This study demonstrates the complementary predictive power of tapping, gait/rest and voice data and establishes integrative deep learning-based models for identifying PD.
Crab nebula at 260 GHz with the NIKA2 polarimeter: Implications for the polarization angle calibration of future CMB experiments
Authors: Ritacco A. , Adam R. , Ade P. , Ajeddig H. , André P. , Artis E. , Aumont J. , Aussel H. , Beelen A. , Benoît A. , Berta S. , Bing L. , Bourrion O. , Calvo M. , Catalano A. , De Petris M. , Désert F.-X. , Doyle S. , Driessen E. F. C. , Gomez A. , Goupy J. , Kéruzoré F. , Kramer C. , Ladjelate B. , Lagache G. , Leclercq S. , Lestrade J.-F. , Macías-Pérez J.-F. , Maury A. , Mauskopf P. , Mayet F. , Monfardini A. , Muñoz-Echeverría M. , Perotto L. , Pisano G. , Ponthieu N. , Revéret V. , Rigby A. J. , Romero C. , Roussel H. , Ruppin F. , Schuster K. , Shu S. , Sievers A. , Tucker C. , Zylka R. 
Published: January 2022

The quest for primordial gravitational waves enclosed in the Cosmic Microwave Background (CMB) polarization B-modes signal motivates the development of a new generation of high sensitive experiments (e.g. CMBS4, LiteBIRD), thus allowing to probe the inflationary epoch in the early Universe. However, this will be only possible by ensuring a high control of the instrumental systematic effects and an accurate absolute calibration of the polarization angle.
The Crab nebula is known to be a polarization calibrator on the sky for CMB experiments. Already used for the Planck satellite it exhibits a high polarized signal at microwave wavelengths. In this work we present Crab polarization observations obtained, in the 260 GHz frequency band, with the NIKA2 instrument. Furthermore, we discuss the accuracy needed on such a measurement to improve the constraints on the absolute angle calibration for CMB experiments.
Kleine Ursache – große Wirkung: Spättyp-Hauttests mit Medikamenten als Auslöser von disseminierten Rezidivexanthemen
Authors: Bircher Andreas J. , Jamiolkowski Dagmar , Steveling-Klein Esther H. , Scherer Hofmeier Kathrin , Mateluna Carlos Morales 
Published: January 2022

ZusammenfassungEs werden zwei Patienten vorgestellt, die wegen eines streuenden allergischen Kontakt- bzw. Arzneimittelexanthems auf Budesonid bzw. Antibiotika abgeklärt wurden. Bei beiden trat innerhalb einiger Stunden nach Anlegen eines Epikutantests mit Budesonid bzw. Intradermaltests mit Amoxicillin eine stark positive Hauttestreaktion sowie ein Exanthem auf. Beim ersten Patienten lag eine Sensibilisierung auf die kreuzreagierenden Moleküle Budesonid und Amcinonid, bei der zweiten Patientin eine ausgeprägte Überempfindlichkeit auf Aminopenicilline vor. Aufflammphänomene (Flare-ups) sind v. a. aus der Kontaktallergologie bekannt, können aber auch bei der Abklärung von allergischen Arzneimittelexanthemen auftreten. Systemische Reaktionen vom verzögerten Typ auf Spättyp-Hauttests mit Medikamenten sind relativ selten, können aber eine erhebliche Morbidität bewirken. Verschiedene Formen von Aufflammphänomenen in der Allergologie werden diskutiert.
Robotisch assistierte totale Gastrektomie mit D2-Lymphadenektomie und intrakorporaler Rekonstruktion
Authors: Hoeppner Jens 
Published: January 2022

ZusammenfassungDie robotisch assistierte totale Gastrektomie beim Magenkarzinom ist eine anspruchsvolle Operation, die in Asien, aber zunehmend auch in Europa in spezialisierten Zentren
durchgeführt wird. Die minimalinvasive Resektion, aber vor allem die Lymphadenektomie und minimalinvasive intrakorporale Ösophagojejunostomie sind technisch anspruchsvolle
operative Schritte, deren Technik international in verschiedenen Variationen durchgeführt wird. In dem vorgestellten Video zeigen wir die robotisch assistierte minimalinvasive
Technik der totalen Gastrektomie mit D2-Lymphadenektomie und intrakorporaler Rekonstruktion mittels Seit-zu-Seit-Ösophagojejunostomie in linearer Staplertechnik. Die
DaVinci-Xi-assistierte Operationstechnik wird detailliert beschrieben und in dem zugehörigen Video demonstriert.
Robotic Surgery for Ulcerative Colitis
Authors: Anderson Marissa , Grucela Alexis 
Published: January 2022

AbstractSubtotal colectomy (STC) or total proctocolectomy (TPC) and ileal pouch–anal anastomosis (IPAA) performed in two or three stages remain the procedure of choice for patients with ulcerative colitis (UC). Minimally invasive laparoscopic approaches for STC and IPAA have been established for over a decade, having been shown to reduce postoperative pain, length of stay, and improve fertility. However “straight-stick” laparoscopy has ergonomic and visual disadvantages in the pelvis, which may contribute to IPAA failure. The robotic platform was developed to overcome these limitations. Robotic STC is associated with lower conversion rates and earlier return of bowel function with acceptably longer operative time (mean, 28 minutes) than laparoscopic STC. The robotic approach has also been shown in case series to be safe in urgent settings. Robotic IPAA is associated with lower blood loss and length of stay than laparoscopic IPAA. Robotic TPC/IPAA has been shown in small case series to be safe and feasible despite longer operating times.
A Privacy-Preserving Distributed Analytics Platform for Health Care Data
Authors: Welten Sascha , Mou Yongli , Neumann Laurenz , Jaberansary Mehrshad , Yediel Ucer Yeliz , Kirsten Toralf , Decker Stefan , Beyan Oya 
Published: January 2022

Abstract
Background In recent years, data-driven medicine has gained increasing importance in terms of diagnosis, treatment, and research due to the exponential growth of health care data. However, data protection regulations prohibit data centralisation for analysis purposes because of potential privacy risks like the accidental disclosure of data to third parties. Therefore, alternative data usage policies, which comply with present privacy guidelines, are of particular interest.
Objective We aim to enable analyses on sensitive patient data by simultaneously complying with local data protection regulations using an approach called the Personal Health Train (PHT), which is a paradigm that utilises distributed analytics (DA) methods. The main principle of the PHT is that the analytical task is brought to the data provider and the data instances remain in their original location.
Methods In this work, we present our implementation of the PHT paradigm, which preserves the sovereignty and autonomy of the data providers and operates with a limited number of communication channels. We further conduct a DA use case on data stored in three different and distributed data providers.
Results We show that our infrastructure enables the training of data models based on distributed data sources.
Conclusion Our work presents the capabilities of DA infrastructures in the health care sector, which lower the regulatory obstacles of sharing patient data. We further demonstrate its ability to fuel medical science by making distributed data sets available for scientists or health care practitioners.
Acute Arteriobiliary Fistula Emergent Long after Biliary Drainage and Radioembolization
Authors: Dökdök Murat , Karaman Kutlay 
Published: January 2022

AbstractVarious causes of hemobilia have been described in the literature, majority being iatrogenic. Other different pathologies such as inflammation and tumors also have been described. A 55-year-old male, who had a history of obstructive jaundice caused by a 4-cm liver mass treated with external-internal biliary drainage and transarterial radioembolization 6 months back, presented with acute hemobilia. Computed tomography demonstrated widespread radionecrosis of the right lobe and extravasation of intravascular contrast from right hepatic artery into common bile duct. Verified on angiography, the arteriobiliary fistula was managed with coil embolization.
Novel Resection System for Direct Endoscopic Necrosectomy of Walled-Off Pancreas Necrosis: Have We Conquered the Final Frontier?
Authors: Rana Surinder Singh , Gupta Rajesh 
Published: January 2022

AbstractSurgical necrosectomy has been the traditional management for pancreatic necrosis and is done using blunt dissection. However, lack of dedicated endoscopic accessories has been a major limitation in direct endoscopic necrosectomy (DEN). Standard endoscopic accessories cannot effectively remove large necrotic material. Also, diameter of instrument channel of the endoscope limits the ability to use large-diameter graspers that can remove large chunks of necrotic material. In this news, we discuss a recent study that has evaluated a new powered endoscopic debridement system for DEN.