bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adolescents and adults with FOXP1 syndrome show high rates of anxiety and externalizing behaviors but not psychiatric decompensation or skill loss
Authors: Levy Tess , Silver Hailey , Soufer Renee , Rouhandeh Audrey , Kolevzon Alexander , Buxbaum Joseph D. , Siper Paige M. 
Published: January 2025

ABSTRACTBackgroundFOXP1 syndrome is a genetic neurodevelopmental disorder associated with complex clinical presentations including global developmental delay, mild to profound intellectual disability, speech and language impairment, autism traits, attention-deficit/hyperactivity disorder (ADHD), and a range of behavioral challenges.. To date, much of the literature focuses on childhood symptoms and little is known about the FOXP1 syndrome phenotype in adolescence or adulthood.MethodsA series of caregiver interviews and standardized questionnaires assessed psychiatric and behavioral features of 20 adolescents and adults with FOXP1 syndrome. Interviews captured change in various psychiatric manifestations over time. Medication, social, educational, and vocational history was collected and visual analog scales measured top caregiver concerns during childhood and adolescence/adulthood.ResultsAnxiety and externalizing behaviors were common in this cohort and psychiatric features, such as psychosis or bipolar symptoms, were present in two participants. There was no reported regression or loss of skills, early in development or during adolescence/adulthood. Caregivers reported continued development in adaptive skills even into adolescence/adulthood. Medication use was common particularly for features of ADHD, although multiple trials were required for some participants to achieve benefit. Standardized assessments accurately picked up on externalizing symptoms and were less sensitive to internalizing symptoms. Educational setting varied up until late elementary school and gradually shifted to special education. Cognitive and developmental concerns were reported as primary during childhood and independence/safety and housing concerns became top concerns by adolescence/adulthood. Caregivers reported continued development in adaptive skills even into adulthood.ConclusionsTaken together, results are reassuring, with many families reporting their adolescent and adult children continued to gain skills over time, particularly related to increased independence in communication and personal care. There were no reports of developmental regression, neuropsychiatric decompensation or catatonia.
The trans-kingdom spectrum of Mpox-like lesion pustules of suspect patients in the Mpox subclade Ib outbreak in Eastern Democratic Republic of the Congo
Authors: Murhula Masirika Leandre , Hewins Benjamin , Toloue Ostadgavahi Ali , Dutt Mansi , Mutimbwa Mambo Léandre , Udahemuka Jean Claude , Ndishymie Pacifique , Bengehya Mbiribindi Justin , Belesi Siangoli Freddy , Kelvin Patricia , Langille Morgan G. I. , Kelvin David J , Flores Luis , Sganzerla Martinez Gustavo , Kumar Anuj 
Published: January 2025

ABSTRACTDuring infectious disease outbreaks, acquiring genetic data across various kingdoms offers essential information to tailor precise treatment methodologies and bolster clinical, epidemiological, and public health awareness. Metagenomics sequencing has paved the way for personalized treatment approaches and streamlined the monitoring process for both co-infections and opportunistic infections. In this study, we conducted long-read metagenomic DNA sequencing on Mpox-like lesion pustules in six suspected patients during the MPXV subclade Ib outbreak in Eastern Democratic Republic of the Congo. The sequenced data was taxonomically classified per the bacterial, fungal, and viral composition. Our results show a wide spectrum of microorganisms present in the lesions. Bacteria such asCorynebacterium amycolatum, Gardnerella vaginalis, Enterococcus faecium, Enterobacter clocae, Staphylococcus epidermidis, andStenotrophomonas maltophiliawere found in the lesions. The viral classification of the reads pointed out the absolute predominance of Monkeypox virus. Taken together, the outcomes of this investigation underscore the potential involvement of microorganisms in mpox lesions and the potential role that co-infections play in exacerbating disease severity and transmission during the MPXV subclade Ib outbreak.
The Evolutionary History of Metallothioneins in Horseshoe Crabs, Scorpions, Spiders and other Chelicerate Species
Authors: Palacios Òscar , Capdevila Mercè , Albalat Ricard 
Published: January 2025

AbstractMetallothioneins (MTs) are a heterogenous family of proteins involved in metal homeostasis and detoxification, playing a crucial role in the adaptation of organisms to varying conditions of metal bioavailability across ecosystems. Despite their significance, MT characterization across animal groups remains uneven. While MTs have been extensively studied in vertebrates and gastropods, their presence and function in many arthropods, particularly within the Chelicerata subphylum, remain largely unexplored. Chelicerates, comprising thousands of species of spiders, scorpions and ticks, are a highly diverse group of animals inhabiting a wide range of environments. Identifying and characterizing their MTs is therefore essential for understanding how they manage metal availability of diverse habitats. In this study, we have identified over 400 chelicerate MTs, classifying them into three distinct types –types 1, 2, and 3– each exhibiting significant structural and phylogenetic differences. Our findings have revealed that most chelicerate MTs are bidomain proteins, with notable exceptions of monodomain forms and larger variants. Evolutionary analysis suggests that type 1 MTs represent the ancestral form, while types 2 and 3 likely emerged during the evolution of spiders. These results pave the way of a deeper understanding of the metal homeostasis and detoxification in chelicerates, laying the groundwork for future studies on the origin, evolution and functional diversification of MTs in arthropods and other metazoan lineages.
Spatial dissimilarity analysis in single cell transcriptomics
Authors: Shi Quan , Kristiansen Karsten 
Published: January 2025

AbstractThe spatial dissimilarity method is a new statistical tool to uncover complex bivariate relationships in single cells and spatial transcriptomics data, thereby addressing unresolved issues such as alternative splicing and allele-specific gene expression. By applying our spatial dissimilarity analysis method on datasets of neurons, tumor, and normal cells, we identified thousands of alternatively spliced genes and instances of allele-specific gene expression. This is particularly evident in the analysis of quiescent and transitioning cell states, where our method reveals the nuanced gene expression dynamics associated with these states. Notably, our findings highlight how allele-specific genetic variants can provide insights into the subclone architecture of normal cells and cancer cells, offering a more comprehensive understanding of cellular heterogeneity and a new insight to understand gene function at specific cell types. The spatial dissimilarity analysis method deepens our comprehension of cellular complexity and gene expression dynamics during cell state transitions.
Decoding the Molecular Language of Proteins with Evola
Authors: Zhou Xibin , Han Chenchen , Zhang Yingqi , Su Jin , Zhuang Kai , Jiang Shiyu , Yuan Zichen , Zheng Wei , Dai Fengyuan , Zhou Yuyang , Tao Yuyang , Wu Dan , Yuan Fajie 
Published: January 2025

AbstractProteins, nature’s intricate molecular machines, are the products of billions of years of evolution and play fundamental roles in sustaining life. Yet, deciphering their molecular language - that is, understanding how protein sequences and structures encode and determine biological functions - remains a cornerstone challenge in modern biology. Here, we introduce Evola, an 80 billion frontier protein-language generative model designed to decode the molecular language of proteins. By integrating information from protein sequences, structures, and user queries, Evola generates precise and contextually nuanced insights into protein function. A key innovation of Evola lies in its training on an unprecedented AI-generated dataset: 546 million protein question-answer pairs and 150 billion word tokens, designed to reflect the immense complexity and functional diversity of proteins. Post-pretraining, Evola integrates Direct Preference Optimization (DPO) to refine the model based on preference signals and Retrieval-Augmented Generation (RAG) for external knowledge incorporation, improving response quality and relevance. To evaluate its performance, we propose a novel framework, Instructional Response Space (IRS), demonstrating that Evola delivers expert-level insights, advancing research in proteomics and functional genomics while shedding light on the molecular logic encoded in proteins. The online demo is available athttp://www.chat-protein.com/.
Mitochondria as Indispensable Yet Replaceable Components of Germ Plasm: Insights into Primordial Germ Cell Specification in Non-Teleost Sturgeons
Authors: Gao Linan , Franěk Roman , Tichopád Tomáš , Rodina Marek , Gela David , Šindelka Radek , Saito Taiju , Pšenička Martin 
Published: January 2025

AbstractWhile it is widely recognized that mitochondria are components of germ plasm, their specific role in the formation and specification of primordial germ cells (PGCs) remains poorly understood. Furthermore, it has not been established whether mitochondria in germ plasm possess unique characteristics essential for their function. In this study, we demonstrate that mitochondria are indispensable for PGCs development in non-teleost fishes and that their role is not dependent on their origin from germ plasm. Using sturgeon embryos, we showed that UV radiation applied to the vegetal pole effectively eliminates germ plasm, including mitochondria, and prevents PGCs formation. Remarkably, we restored germ plasm function and PGCs development by injecting mitochondria derived from donor eggs, even when these mitochondria were not originally part of the germ plasm. Transplanted mitochondria were successfully identified in larval PGCs using a fluorescent PKH26 tracer, and in interspecies transplantation experiments, their presence was confirmed using species-specific mtDNA and mtRNA primers in larvae and individual PGCs. Our findings reveal that mitochondria are critical but not germ plasm-specific determinants of PGCs formation. This study provides novel insights into the developmental pathways of germ cells and establishes a previously unrecognized flexibility in mitochondrial functionality within the germline. These findings also offer a potential method for conserving matrilineal genetics in critically endangered species like sturgeons, while simultaneously opening new avenues for studying germlines with high interspecies mitochondrial heteroplasmy and contributing to broader evolutionary and conservation biology.
An unexpected mode of whole-body regeneration from reaggregated cell suspension inHydractinia(Cnidaria, Hydrozoa)
Authors: Curantz Camille , Krasovec Gabriel , Horkan Helen R , Ryan Laura , Varley Áine , Frank Uri 
Published: January 2025

SUMMARYHydrozoan cnidarians are among the few animals that can regenerate whole bodies from reaggregated cell dissociations but the cellular and molecular mechanisms that control this ability and how it is related to embryonic development are not well understood. Furthermore, the evolution of this type of regeneration is enigmatic since it does not occur naturally. Here, we show that aggregate regeneration inHydractinia symbiolongicarpusproceeds through several, consistent stages that include the formation of an epidermal layer, followed by migration, proliferation, and differentiation of adult pluripotent stem cells, known as i-cells. Migration of i-cells is controlled by sphingosine-1-phosphate signaling. Single-cell transcriptomics revealed, surprisingly, that the newly regenerated individual derives nearly exclusively from i-cell progeny rather than from recycled somatic cells, as seen in other hydrozoans. Given the similarity of this phenomenon to embryogenesis, we propose that the ability ofHydractiniacell aggregates to regenerate is a side effect of the animal’s i-cell-mediated development.
Toward an evolving framework for responsible AI for credit scoring in the banking industry
Authors: Mathen Manoj Philip , Paul Anindita 
Published: January 2025

Purpose
The aim of this research is to conduct a systematic review of the literature on responsible artificial intelligence (RAI) practices within the domain of AI-based Credit Scoring (AICS) in banking. This review endeavours to map the existing landscape by identifying the work done so far, delineating the key themes and identifying the focal points of research within this field.

Design/methodology/approach
A database search of Scopus and Web of Science (last 20 years) resulted in 377 articles. This was further filtered for ABDC listing, and augmented with manual search. This resulted in a final list of 53 articles which was investigated further using the TCCM (Theory, Context, Characteristics and Methodology) review protocol.

Findings
The RAI landscape for credit scoring in the banking industry is multifaceted, encompassing ethical, operational and technological dimensions. The use of artificial intelligence (AI) in banking is widespread, aiming to enhance efficiency and improve customer experience. Based on the findings of the systematic literature review we found that past studies on AICS have revolved around four major themes: (a) Advances in AI technology; (b) Ethical considerations and fairness; (c) Operational challenges and limitations; and (d) Future directions and potential applications. The authors further propose future directions in RAI in credit scoring.

Originality/value
Earlier studies have focused on AI in banking, credit scoring in isolation. This review attempts to provide deeper insights, facilitating the development of this key field.
Halal standards practices in the food industry: the impact of halal assurance and operations
Authors: Othman Baharudin , Md Nawi Nurul Hudani 
Published: January 2025

Purpose
The purpose of this study is to examine the effect of halal standards practices towards organizational performance. Additionally, the moderating role of organizational size on this association is explored for Malaysian halal industry so that the halal management practice in the future is expected to run well.

Design/methodology/approach
Regression analyses technique via Statistical Package For Social Science 27.0 is used to analyses the results of a self-administered questionnaire completed by 241 internal halal committee officers of multinational corporations and small and medium enterprises (SMEs) in Malaysia.

Findings
The results showed that halal standards practices give an effect that indicates 13.2% of the performance. In more detail, it only demonstrates the significant contribution of the halal assurance predictor to organizational performance compared to the halal operations (HOs) predictor. Additionally, organizational size also acts as a moderator between the relationships between halal standard practices (HO) and organizational performance. Besides, the study findings also show that there was a significant difference in organizational performance for multinationals and SMEs. This encouraging outcome is the result of organizational performance is influenced by a chain that interacts with each other starting from input, which then translates into process and ultimately results that are also supported by internal control of the organization for Malaysian halal industry.

Research limitations/implications
This study only focused on the Malaysian halal industry such as certain number of factors and limited measurement was used in this study. Only a few halal standards practices and organization performance measurements were considered.

Practical implications
Halal food producers should engender greater commitment to Halal standard practice involving acts, standards, regulations and guidelines that can be used to comply halal requirements needed by halal certification bodies especially halal committee members to control internally. It also serves as a valuable resource for policymakers and practitioners aiming to identify effective practices for comprehensive halal standards management to enhance organizational performance, particularly for micro-scale enterprises within the Malaysian halal industry.

Originality/value
This study considerably advances the existing body of knowledge by applying standard halal practices model to explain their relationship, with organization size serving as a moderating to variable and provide direction for researchers in subsequent studies. They also offer valuable insights for practitioners seeking to continually enhance organizational performance through the implementation of effective halal standards management practices within the Malaysian halal industry.
Disentangling Timing Uncertainty of Event‐Driven Connectedness Among Oil‐Based Energy Commodities
Authors: Kočenda Evžen , Bartušek Daniel 
Published: January 2025

AbstractReported news events frequently influence the pricing dynamics of oil‐based commodities. We analyse almost 900 oil‐related events from 1987 to 2022, categorising them based on recurring characteristics. We quantify dynamic connectedness among energy commodities and apply a novel bootstrap‐after‐bootstrap testing procedure to identify 21 statistically significant historical events that triggered abrupt and enduring increases in volatility connectedness. Geopolitical events are more consistently associated with elevated connectedness than economic events, while natural events do not exhibit a similar impact. Events share prevailing characteristics: their negativity, unexpected nature and the introduction of concerns about oil supply shortages.
Exploring Paramedicine Models of Care: A Novel Macro Analysis Using the RESPIGHT Framework
Authors: Weber Anthony , Devenish Scott , Lam Louisa 
Published: January 2025

ABSTRACTObjectiveTo evaluate the alignment of undergraduate paramedicine curricula in Australia with the RESPIGHT model, focusing on diverse aspects such as emergency response, community engagement and governance.DesignDeductive thematic analysis was conducted to assess the alignment of curricula from 16 Australian universities with the RESPIGHT framework.SettingThe study reviewed undergraduate pre‐registration paramedicine programmes across Australian universities.ParticipantsThe study included a comprehensive analysis of curricula from 16 universities offering undergraduate paramedicine programmes.Main Outcome MeasureThe degree of alignment of paramedicine curricula with the RESPIGHT categories: emergency response, community engagement, situated practice, primary healthcare, integration with health services, governance, leadership and treatment and transport.ResultsThe analysis revealed varying degrees of alignment across the RESPIGHT categories, with notable discrepancies in areas such as community engagement and governance. Some universities demonstrated strong alignment in emergency response and treatment, while others showed weaker integration with primary healthcare and community engagement.ConclusionThe study highlights significant disparities in the preparedness of paramedicine students for the broad scope of practice required in modern healthcare. The findings underscore the need for curriculum reform to better prepare students for community care models and the evolving healthcare landscape, ensuring a more comprehensive and integrated approach to paramedicine education in Australia.
mRNA‐laden lipid nanoparticle‐enabled humanized CD19 CAR‐T‐cell engineering for the eradication of leukaemic cells
Authors: Chen Zhaozhao , Ren Anqi , Li Yingying , Shu Jinhui , Wu Jianghua , Huang Hekuan , Wang Jingming , Hu Yu , Mei Heng 
Published: January 2025

SummaryChimeric antigen receptor T‐cell (CAR‐T) therapy has shown transformative potential in treating malignant tumours, with increasing global approval of CAR‐T products. However, high‐production costs and risks associated with viral vector‐based CAR‐T cells—such as insertional mutagenesis and secondary tumour formation—remain challenges. Our study introduces an innovative CAR‐T engineering approach using mRNA delivered via lipid nanoparticles (LNPs), aiming to reduce costs and enhance safety while maintaining strong anti‐tumour efficacy. We developed an LNP‐based transfection protocol for efficient delivery of mRNA encoding full‐human CAR constructs, achieving high CAR expression and significant cytotoxicity against leukaemic cells in vitro. Co‐culture with Raji cells showed increased cytokine secretion and tumour cell killing by mRNA‐LNP CAR‐T cells. Therapeutic efficacy was further demonstrated in an NOD‐scid‐IL2Rγnull (NSG) mouse model with Raji engraftment, where treated mice exhibited marked tumour regression and extended survival. These findings underscore the potential of mRNA‐LNPs as a non‐viral, effective CAR‐T engineering platform, offering a promising alternative to traditional methods that could improve CAR‐T safety, efficacy and accessibility in clinical cancer immunotherapy.
Dexmedetomidine Inhibits Ferroptosis by Regulating the SRY‐Box Transcription Factor 9/Divalent Metal Transporter‐1 Axis to Alleviate Cerebral Ischemia/Reperfusion Injury
Authors: Liu Zhen , Liu Qionghua , Zhang Xuekang , Li Gan 
Published: January 2025

ABSTRACTCerebral ischemia/reperfusion injury (IRI) is pathologically associated with ferroptosis. Dexmedetomidine (Dex) exerts neuroprotective activity after cerebral IRI. Our work focused on probing the pharmacologic effect of Dex on ferroptosis during cerebral IRI and the mechanisms involved. Cerebral IRI models were established by oxygen–glucose deprivation/reoxygenation (OGD/R) and middle cerebral artery occlusion (MCAO). 2,3,5‐Triphenyltetrazolium chloride (TTC) staining was utilized to detect cerebral infarct size and mNSS was performed to evaluate neurologic deficits. Brain pathologic changes were analyzed by HE staining. Lipid peroxidation level was detected by C11‐BODIPY staining, and Fe2+ and MDA levels were measured using the kits. Cell vitality was examined by CCK‐8 assay. Dual‐luciferase reporter and ChIP assays were adopted to determine the interaction between SOX9 and DMT1 promoter. Dex ameliorated ferroptosis and neuronal death induced by MCAO and OGD/R. SOX9 upregulation abolished the inhibitory effect of Dex on OGD/R‐induced ferroptosis and neuronal death in SH‐SY5Y cells. Our further trials showed that SOX9 transcriptionally activated DMT1 expression. As expected, DMT1 overexpression prevented Dex‐induced decrease in ferroptosis and neuronal death in OGD/R‐treated SH‐SY5Y cells. Dex inhibited ferroptosis to exert neuroprotection effects on cerebral IRI by inactivating the SOX9/DMT1 axis.
Invoking the Discourse of Children's Rights in Campaigns Around Public Space
Authors: Powell Rhian , Muddiman Esther , Power Sally , Taylor Chris 
Published: January 2025

ABSTRACTIncreasing emphasis is being placed on the need to consider children's rights in planning decisions. However, the way these rights are claimed by adults can be problematic, especially when planning decisions are controversial and contested. Drawing on interview data with participants engaged in campaigning around the development of a green space in Cardiff, we show how the adult appropriation of children's rights to support such campaigns may not only be misleading but potentially damaging. We conclude by discussing the limits of age‐based rights claims and the need to ensure that children's rights are not appropriated for adult agendas.