bluuebunny/crossref_metadata_2025_split huggingface dataset:
Twitter Activists’ Argumentation Through Subdiscussions: Theory, Method and Illustration of the Controversy Surrounding Sustainable Fashion
Authors: Greco Sara 
Published: September 2022

Abstract“Why are millions of dollars worth of orders being left unpaid?”. With tweets like this questioning brands’ policies, activists advocating for sustainable fashion re-discuss material starting points that are assumed by fashion brands, who argue that they are sustainable because they care about their workers’ conditions. This paper argues that activists use tweets to open subdiscussions on material starting points to engage citizens and consumers, re-discussing factual data that brands take for granted, such as the fact that they provide fair conditions for their garment workers. Activists justify their opening of subdiscussions, often through an argumentative pattern that includes an argument based on the locus from effects to cause. They argue that if there are negative effects, the brand cannot claim to care about the conditions of its workers. In discussing how subdiscussions are used by fashion activists, this paper also introduces a conceptualization of Twitter argumentation as a discussion that is not isolated, but is part of a polylogical argumentation that takes place in different venues. For this reason, the argumentation used in tweets is reconstructed as a response to a fashion brand’s communication campaigns around sustainability, which extend beyond the confines of Twitter. As an empirical illustration, this paper is based on the campaign targeting fashion retailer Primark; the dataset includes the brand’s website as well as activists’ tweets.
Thermal stress and mutation accumulation increase heat shock protein expression in Daphnia
Authors: Scheffer Henry , Coate Jeremy E. , Ho Eddie K. H. , Schaack Sarah 
Published: September 2022

AbstractUnderstanding the short- and long-term consequences of climate change is a major challenge in biology. For aquatic organisms, temperature changes and drought can lead to thermal stress and habitat loss, both of which can ultimately lead to higher mutation rates. Here, we examine the effect of high temperature and mutation accumulation on gene expression at two loci from the heat shock protein (HSP) gene family, HSP60 and HSP90. HSPs have been posited to serve as ‘mutational capacitors’ given their role as molecular chaperones involved in protein folding and degradation, thus buffering against a wide range of cellular stress and destabilization. We assayed changes in HSP expression across 5 genotypes of Daphnia magna, a sentinel species in ecology and environmental biology, with and without acute exposure to thermal stress and accumulated mutations. Across genotypes, HSP expression increased ~ 6× in response to heat and ~ 4× with mutation accumulation, individually. Both factors simultaneously (lineages with high mutation loads exposed to high heat) increased gene expression ~ 23×—much more than that predicted by an additive model. Our results corroborate suggestions that HSPs can buffer against not only the effects of heat, but also mutations—a combination of factors both likely to increase in a warming world.
Concise review of the macroalgal species Pelvetia canaliculata (Linnaeus) Decaisne & Thuret
Authors: Lalegerie Fanny , Stengel Dagmar B. 
Published: September 2022

AbstractPelvetia canaliculata (L.) Dcne. et Thur. is a marine brown macroalga within the Fucaceae (Fucales, Phaeophyceae, Ochrophyta) and the only species remaining in the monotypic genus Pelvetia. Abundant on sheltered rocky shores of the North-East Atlantic, Pelvetia forms a distinct horizontal band along the upper fringe in the intertidal, between maritime lichens and Fucus spp. Pelvetia canaliculata is a perennial species, characterized by dichotomously branching thalli 10–15 cm in length, forming narrow channels (“channelled wrack”). Distributed on the extreme upper shore, the species is exposed to atmospheric conditions for prolonged periods between spring high tides; photosynthesis recovers quickly, and fully, after water loss. Effective de-epoxidation of the xanthophyll cycle observed in P. canaliculata constitutes an important photoprotective mechanism in this species which receives high doses of solar radiation during tidal emersion. In comparison with other intertidal brown macroalgae, only few studies have investigated its chemical composition. Pelvetia typically presents high levels of sulphated polysaccharides, alginic acid, and polyphenols, associated with antioxidant and other biological activities. It accumulates high levels of mannitol and volemitol, two sugar alcohols considered to be involved in its tolerance to extended emersion. As a natural cation exchanger, Pelvetia biomass displays high biosorption capacities for toxic metals. Besides its slow growth rate and related small quantities of biomass, low protein content coupled with low degradability, have so far limited its potential for valorization.
Suppression of trinucleotide repeat expansion in spermatogenic cells in Huntington’s disease
Authors: Cho In K. , Easley Charles A. , Chan Anthony W. S. 
Published: September 2022

AbstractTrinucleotide repeats (TNRs) are dispersed throughout the human genome. About 20 loci are related to human diseases, such as Huntington’s disease (HD). A larger TNR instability is predominantly observed in the paternal germ cells in some TNR disorders. Suppressing the expansion during spermatogenesis can provide a unique opportunity to end the vicious cycle of genetic anticipation. Here, using an in vitro differentiation method to derive advanced spermatogenic cells, we investigated the efficacy of two therapeutic agents, araC (cytarabine) and aspirin, on stabilizing TNRs in spermatogenic cells. Two WT patient-derived induced pluripotent stem cell (iPSC) lines and two HD hiPSC lines, with 44 Q and 180 Q, were differentiated into spermatogonial stem cell-like cells (SSCLCs). Both HD cell lines showed CAG tract expansion in SSCLC. When treated with araC and aspirin, HD1 showed moderate but not statistically significant stabilization of TNR. In HD2, 10 nM of aspirin and araC showed significant stabilization of TNR. All cell lines showed increased DNA damage response (DDR) gene expression in SSCLCs while more genes were significantly induced in HD SSCLC. In HD1, araC and aspirin treatment showed general suppression of DNA damage response genes. In HD2, onlyFAN1,OGG1, andPCNAshowed significant suppression. When the methylation profile of HD cells was analyzed,FAN1andOGG1showed significant hypermethylation after the aspirin and araC treatment in SSCLC compared to the control. This study underscores the utility of our in vitro spermatogenesis model to study and develop therapies for TNR disorders such as HD.
Mechanical response during bending of Ni–Mn–Ga-based melt-spun ribbons
Authors: Czaja P. , Kowalska M. , Brzoza-Kos A. , Szczerba M. J. 
Published: September 2022

AbstractIn this paper, we report on mechanical properties observed during bending experiments conducted on quinary Ni–Mn–Ga–Co–Cu melt-spun ribbons. Depending on the ribbon’s side to which force is applied, different mechanical response is noted. Substantially larger mechanical instabilities are observed when force is applied to the “free side” than to the “wheel side” of ribbons. When force is applied to the latter, much lower force fluctuations are recorded and the amplitude of the force–displacement response remains within the experimental resolution limit. It is also shown that the character of the force–displacement curve changes upon cycling; mainly by decreasing the maximum force and mechanical hysteresis. These results are important for materials design and optimization of the magnetic field-induced bending effect recently shown in Ni–Mn–Ga-based melt-spun ribbons.
A lack of a definite correlation between male sub-fertility and single nucleotide polymorphisms in sperm mitochondrial genes MT-CO3, MT-ATP6 and MT-ATP8
Authors: Saleh Jaweesh Mayyas , Hammadeh Mohamad Eid , Dahadhah Fatina W. , Al Smadi Mohammad A. , Al Zoubi Mazhar Salim , Alarjah Manal Issam Abu , Amor Houda 
Published: September 2022

Abstract
Background
An inability of a man to conceive a potentially fertile woman after a year of unprotected intercourse is defined as male infertility. It is reported that 30–40% of males in their reproductive years have abnormalities in sperm production, either qualitatively or quantitatively, or both. However, genetic factors result in up to 15% of male infertility cases. The present study aimed to analyze the possible correlations between sub-fertility and polymorphisms in sperm mitochondrial CO3, ATP6 and ATP8 genes in sub-fertile men.

Methods and results
For 67 sub-fertile and 44 fertile male samples, Sanger sequencing of selected mitochondrial DNA genes was done. A total of twelve SNPs in the MT-CO3 gene: rs2248727, rs7520428, rs3134801, rs9743, rs28358272, rs2853824, rs2856985, rs2854139, rs41347846, rs28380140, rs3902407, and 28,411,821, fourteen SNPs in the MT-ATP6: rs2001031, rs2000975, rs2298011, rs7520428, rs9645429, rs112660509, rs6650105, rs6594033, rs6594034, rs6594035, rs3020563, rs28358887, rs2096044, and rs9283154, and ten SNPs in the MT-ATP8: rs9285835, rs9285836, rs9283154, rs8179289, rs121434446, rs1116906, rs2153588, rs1116905, rs1116907, and rs3020563 were detected in the case and control groups at different nucleotide positions. Only the rs7520428 in the MT-CO3 and MT-ATP6 showed a statistically significant difference between sub-fertile and fertile groups in the genotype’s and allele’s frequency test (P < 0.0001 for both).

Conclusion
The results of our study suggest that male sub-fertility is linked with rs7520428 SNP in MT-CO3 and MT-ATP6. The studied polymorphic variations in the MT-ATP8 gene, on the contrary, did not reveal any significant association with male sub-fertility.
Neuroblastoma SH-SY5Y Cell Differentiation to Mature Neuron by AM580 Treatment
Authors: Cai Aojie , Lin Zehong , Liu Nana , Li Xiao , Wang Jingmin , Wu Ye , Gao Kai , Jiang Yuwu 
Published: September 2022

AbstractNeuroblastoma is a type of developmental childhood cancer that arises from the neural crest. It is the most common pediatric solid tumor in the world. AM580 is a powerful cyto-differentiating molecule on acute promyelocytic leukemia cells and induced pluripotent stem cells, but its effect on neuroblastoma is still unknown. In this study, the neuronal differentiation impact of AM580 was investigated using the human neuroblastoma cell line SH-SY5Y as a model. AM580 successfully stimulated the SH-SY5Y cells to develop into neuron-like cells. Functional enrichment analysis of RNAseq data revealed that differentially expressed genes (DEGs) were substantially enriched for GO keywords and KEGG pathways linked to neuron development. Some potassium ion channel genes associated with neuronal excitation, such as KCNT1, were shown to be upregulated. Through the MEA tests, we found the AM580-induced neurons possessed electrical spikes as mature neurons. AM580 also induced the neuronal marker β-tubulin III and mature neurons marker Neurofilament H. Our study proved that AM580 can promote the differentiation of neurons and has the potential to treat neuroblastoma, neurodevelopmental and neurodegenerative diseases.
Attitudes and Beliefs Associated with Cyberbullying and Non-Consensual Sexting in Cisgender and Transgender Adults
Authors: Mussap Alexander J. , Clancy Elizabeth M. , Klettke Bianca 
Published: September 2022

AbstractCyberbullying and non-consensual sexting are prevalent and potentially harmful online behaviours. However, little is known about the attitudes and beliefs that underpin these behaviours in ciswomen and cismen and the extent to which they explain the online experiences of trans and gender diverse (TGD) people. A sample of 638 ciswomen, 722 cismen, and 146 TGD adults 18 to 66 years of age (M = 23.27, SD = 3.66), completed a survey of online perpetration behaviours, victimization experiences, and positive attitudes/beliefs about cyberbullying and sexting. MANCOVAs revealed significant gender differences in terms of both cyber and sexting perpetration and victimization. On average, ciswomen reported 8% less cyberbullying perpetration and 17% less non-consensual sexting perpetration than cismen, and experienced 77% more victimization from non-consensual sexting. TGD adults similarly reported 8% less cyberbullying perpetration than cismen, but also 65% less non-consensual sexting perpetration than cismen, as well as experiencing 77% more victimization from non-consensual sexts. MANCOVAs also revealed that cismen held more positive attitudes and beliefs about cyberbullying and sexting than ciswomen and TGD adults. Multigroup path analyses further revealed that positive attitudes and beliefs were related to perpetration behaviours but differently for different genders, with pro-cyberbullying attitudes/beliefs associated with perpetration behaviours in TGD adults, and pro-sexting attitudes/beliefs associated with perpetration behaviours in cisgender adults. These results highlight gender differences in online perpetration and victimization, extend this observation to TGD populations, and demonstrate the importance of underlying attitudes and beliefs.
A Versatile Micromanipulation Apparatus for Biophysical Assays of the Cell Nucleus
Authors: Currey Marilena L. , Kandula Viswajit , Biggs Ronald , Marko John F. , Stephens Andrew D. 
Published: September 2022

Abstract
Intro
Force measurements of the nucleus, the strongest organelle, have propelled the field of mechanobiology to understand the basic mechanical components of the nucleus and how these components properly support nuclear morphology and function. Micromanipulation force measurement provides separation of the relative roles of nuclear mechanical components chromatin and lamin A.

Methods
To provide access to this technique, we have developed a universal micromanipulation apparatus for inverted microscopes. We outline how to engineer and utilize this apparatus through dual micromanipulators, fashion and calibrate micropipettes, and flow systems to isolate a nucleus and provide force vs. extensions measurements. This force measurement approach provides the unique ability to measure the separate contributions of chromatin at short extensions and lamin A strain stiffening at long extensions. We then investigated the apparatus’ controllable and programmable micromanipulators through compression, isolation, and extension in conjunction with fluorescence to develop new assays for nuclear mechanobiology.

Results
Using this methodology, we provide the first rebuilding of the micromanipulation setup outside of its lab of origin and recapitulate many key findings including spring constant of the nucleus and strain stiffening across many cell types. Furthermore, we have developed new micromanipulation-based techniques to compress nuclei inducing nuclear deformation and/or rupture, track nuclear shape post-isolation, and fluorescence imaging during micromanipulation force measurements.

Conclusion
We provide the workflow to build and use a micromanipulation apparatus with any inverted microscope to perform nucleus isolation, force measurements, and various other biophysical techniques.
Case Series Analysis of Male Breast Cancer
Authors: Li Chao , Li Xujun 
Published: September 2022

Abstract
Male breast cancer is a rare disease, accounting for less than 1% of all cases of breast cancer. However, the incidence of male breast cancer is increasing. In this study, we systematically analyzed the cases of male breast cancer and evaluated the management of operable male breast cancer in daily clinical practice according to the international clinical guidelines. Data of male breast cancer characteristics and management were recorded from our electronic database. We present a case series of male breast cancer patients treated at our institution between 2013 and 2021 and summarize the recommendations on management of male patients with breast cancer. Twenty-six patients met the search criteria. The majority of tumors were hormone-receptor positive and human epidermal growth factor receptor 2 negative. Two tumors were derived from axillary accessory breast. All patients underwent mastectomy. Fifteen patients received adjuvant chemotherapy containing anthracycline or taxane. Ten patients received adjuvant radiotherapy for lymph node metastasis. Twenty-five patients received adjuvant endocrine therapy. None of patients received anti-human epidermal growth factor receptor 2 therapy. During the median follow-up period of 37 months, one patient died of previous lung cancer recurrence and one patient developed lung metastases 2 years after breast surgery. No recurrence or distant metastasis occurred in the other twenty-four patients. The incidence of male breast cancer is low. In this study, we present twenty-six cases of male patients with operable breast cancer in our department. All of them received surgery and adjuvant treatment. Early diagnosis and combined therapy benefit male patients with breast cancer. Surgery together with adjuvant treatment can bring a good prognosis.
Automatic event detection in football using tracking data
Authors: Vidal-Codina Ferran , Evans Nicolas , El Fakir Bahaeddine , Billingham Johsan 
Published: September 2022

AbstractOne of the main shortcomings of event data in football, which has been extensively used for analytics in the recent years, is that it still requires manual collection, thus limiting its availability to a reduced number of tournaments. In this work, we propose a deterministic decision tree-based algorithm to automatically extract football events using tracking data, which consists of two steps: (1) a possession step that evaluates which player was in possession of the ball at each frame in the tracking data, as well as the distinct player configurations during the time intervals where the ball is not in play to inform set piece detection; (2) an event detection step that combines the changes in ball possession computed in the first step with the laws of football to determine in-game events and set pieces. The automatically generated events are benchmarked against manually annotated events and we show that in most event categories the proposed methodology achieves $$+90\%$$

+
90
%

detection rate across different tournaments and tracking data providers. Finally, we demonstrate how the contextual information offered by tracking data can be leveraged to increase the granularity of auto-detected events, and exhibit how the proposed framework may be used to conduct a myriad of data analyses in football.
Identification of Q value and angular frequency of smart gear antenna using the resonant return loss of the receiving antenna
Authors: Mac Thanh-Tung , Iba Daisuke , Matsushita Yusuke , Inoue Takeru , Miura Nanako , Masuda Arata , Moriwaki Ichiro 
Published: September 2022

Abstract
A wireless smart gear system has been being developed as a gear health monitoring system. This system works based on the magnetic coupling between its two major elements consisting of a monitoring antenna and a smart gear. In operation, the smart gear works as a working gear in the power transmission system while the monitoring antenna monitors this gear continuously. Specifically, since the monitoring antenna is fixed and kept connecting to a network analyzer during the gear operation, it allows the magnetic coupling to occur and remain thoroughly. As a result, the return loss signal of the monitoring antenna becomes a resonant return loss data of the smart gear system. In this paper, the authors propose a method to use this resonant return loss signal for identifying two parameters called the $$Q$$
Q
value and the angular frequency of the smart gear side antenna. There are three cases of magnetic coupling that have been considered. These cases are called Open, Short, and Loaded by a 50 [Ω] resistor where the characteristics of the antenna on the smart gear side are changed via its connector by leaving Open or connecting to a Short and a 50 [Ω] resistor modules, respectively. The results indicate that the method is effective for parameter identification of the $$Q$$
Q
value and the angular frequency of the smart gear side antenna for all three cases. Moreover, the analysis of the validated $$Q$$
Q
value and the angular frequency also indicates the relation between these two characteristic values and the practical conditions of the smart gear. This finding consolidates the effectiveness of our developing wireless smart gear system.

Article Highlights

This article proposes a parameter identification method for a developing smart gear health monitoring system. By only using the resonant return loss data obtained from the monitoring antenna, there are two characteristic values of the smart gear have been achieved.

This article also introduces a completed return loss equation of a magnetically coupled system. This return loss equation is helpful for the computation of characteristic parameters of the magnetically coupled system such as a wireless power transfer system or a coupled antenna system.

The results figure out that the identified characteristic parameters and the physical condition of the smart gear side’s antenna circuit are related to each other. This finding affirms the effectiveness of not only the proposed parameter identification method but also the developing smart gear system.
Recycling Interdisciplinary Evidence: Abandoned Hypotheses and African Historiologies in the Settlement History of Littoral East Africa
Authors: Ray Daren E. 
Published: September 2022

AbstractScholars can elevate African voices as they recycle evidence from abandoned lines of research. This article discusses how to apply the confirmation and recycling methods of interdisciplinary research to engage with African historiologies. After reviewing contentious debates about Shungwaya from ca. 1955–2000, it draws on Mijikenda elder Thomas Govi’s descriptions of uganga and clanship (in a published collection of oral traditions) as a historiological theory for reimagining cross-linguistic collaborations, the formation of “stone towns,” and Islamic conversion in the settlement history of littoral East Africa.
Critical Junctures and Ontological Security in Unrecognized States: The Response of Northern Cyprus to the COVID-19 Pandemic
Authors: Gülseven Enver 
Published: September 2022

AbstractThis article will examine the response of the self-declared Turkish Republic of Northern Cyprus (TRNC) to the COVID-19 pandemic from an ontological security approach to illustrate how critical junctures may help de facto states in their quest for status and internal legitimacy. Stressing the pandemic’s role in the reconstruction of political narratives and self-legitimating practices among Turkish Cypriot elites, it sheds light on the effects of this global crisis on domestic power struggles in Northern Cyprus as well as its quasi-foreign relations with its patron state (Turkey), parent state (Republic of Cyprus), and the European Union. The study shows that both nationalist and federalist elites of the de facto entity instrumentalized the pandemic in their legitimation strategies and engaged in opportunistic behavior amid the outbreak. It also reveals how the pandemic enhanced the ontological security of Northern Cyprus while advancing its nationalist leadership’s claims for legitimate authority by enabling state-specific forms of agency and unilateral acts concerning the Cyprus dispute that may jeopardize the resumption of peace talks with Greek Cypriots. Thus, it can be assumed that advanced ontological security of the TRNC is highly volatile given the prospects of further isolation and de-engagement in the post-pandemic era.