bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a novel feeding method for Japanese black calves with thermophile probiotics at postweaning
Authors: Inabu Yudai , Taguchi Yutaka , Miyamoto Hirokuni , Etoh Tetsuji , Shiotsuka Yuji , Fujino Ryoichi , Okada Toru , Udagawa Motoaki , Tsuji Naoko , Matsuura Makiko , Tsuboi Arisa , Kato Tamotsu , Kodama Hiroaki , Ohno Hiroshi , Takahashi Hideyuki 
Published: March 2022

Abstract

Aims
Probiotic effects of compost containing thermophiles on productivity have been reported in domestic animals, although not cattle. We evaluated the effects of administering Caldibacillus hisashii, a thermophile contained in compost, on growth, blood components, faecal organic acid concentrations and microbiota population in Japanese black calves.

Methods and results
Calves were administered C. hisashii from 3 to 5 months of age. Administering C. hisashii decreased feed intake without affecting body weight, indicating that feed efficiency is improved by administration. Administering C. hisashii decreased plasma insulin concentration without affecting glucose and non-esterified fatty acid concentrations. Chao1 was decreased by exposure at 5 months of age. Similarly, weighted and unweighted UniFrac distances were affected by treatment at 5 months of age. Faecal abundance of the phylum Bacteroidetes tended to be increased by exposure. Faecal propionic acid concentration was correlated positively with faecal abundance of phylum Bacteroidetes but negatively with that of Firmicutes. Interestingly, the population of the genus Methanobrevibacter, representing the majority of methanogens, was lowered by exposure and was negatively correlated with faecal propionic acid concentration.

Conclusion
Administration of C. hisashii has the potential to improve growth performance of Japanese black calves and to contribute to reducing environmental load, which may be associated with altered endocrine kinetics and gut microbial populations.

Significance and Impact of the Study
This study revealed that isolated thermophiles included in compost may exert probiotic effects on calves.
Reactive oxygen species‐induced SIAH1 promotes granulosa cells' senescence in premature ovarian failure
Authors: Lin Li , Gao Wujiang , Chen Yumei , Li Taoqiong , Sha Chunli , Chen Lu , Yang Meiling , Wei Hong , Chen Yunpeng , Zhu Xiaolan 
Published: March 2022

AbstractReactive oxygen species (ROS) exposure triggers granulosa cells' (GCs) senescence, which is an important causal factor for premature ovarian failure (POF). However, underlying mechanism in this process remains unknown. In our study, we observed increased ROS levels in POF ovarian tissues, POF patient follicular GCs and cyclophosphamide (CTX) pretreated GCs. Correspondingly, increased SIAH1, reduced TRF2 and GC senescence were also found in these cases. Silencing of SIAH1 rescued ROS‐induced TRF2 reduction and cell senescence in GCs. Moreover, SIAH1 co‐localized with TRF2 in the cytoplasm, facilitating its ubiquitination degradation, further leading to telomere abnormalities in GCs. In conclusion, our findings indicate that ROS induces telomere abnormalities by augmenting SIAH1‐mediated TRF2 degradation, leading to cell senescence in GCs in POF processing.
Naturalized Dolichogenidea gelechiidivoris complement the resident parasitoid complex of Tuta absoluta in North‐eastern Spain
Authors: Denis Carmen , Riudavets Jordi , Alomar Oscar , Agustí Nuria , Gonzalez‐Valero Helena , Cubí Martina , Matas Montserrat , Rodríguez David , van Achterberg Kees , Arnó Judit 
Published: March 2022

AbstractOur work reports on the establishment of the neotropical parasitoid Dolichogenidea gelechiidivoris Marsh (Hymenoptera: Braconidae) for the first time in Europe. This larval parasitoid has been recorded in samples collected in commercial tomato crops in Catalonia (North‐eastern Spain) from 2016 to the present. Dolichogenidea gelechiidivoris is considered to be a new biocontrol agent among the resident parasitoid complex of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae).
Faecal immunochemical test mitigates risk of delayed colonoscopy in people with elevated risk of colorectal neoplasia
Authors: Wassie Molla M , Young Graeme P , Cock Charles , Bampton Peter , Winter Jean M , Simpson Kalindra , Saluja Hariti , Chuang Anthony , Fraser Robert J , Symonds Erin L 
Published: March 2022

AbstractBackground and AimSurveillance colonoscopies may be delayed because of pressure on resources, such as the COVID‐19 pandemic. This study aimed to determine whether delayed surveillance colonoscopy increases the risk for advanced neoplasia and whether interval screening with faecal immunochemical tests (FITs) and other known risk factors can mitigate this risk.MethodsA retrospective cohort study of individuals undergoing surveillance colonoscopy for personal or family history of colorectal neoplasia was being provided with FIT between colonoscopies. Colonoscopy ≥ 6 months after the guideline‐recommended interval was considered “delayed.” Individuals were stratified based on prime colonoscopy findings to nonneoplastic findings, non‐advanced adenoma, and advanced adenoma. The relative risk (RR) for developing advanced neoplasia was determined using a robust multivariable modified Poisson regression.ResultsOf 2548 surveillance colonoscopies, 1457 (57.18%) were delayed. Prior advanced adenoma, older age (> 60 years) and nonparticipation in interval FIT were associated with increased risk for advanced neoplasia (P < 0.05). There was a trend to increased risk in those with prior advanced adenoma with an increasing colonoscopy delay (P trend = 0.01). In participants who did not complete interval FIT and having advanced adenoma in the prime colonoscopy, risk of advanced neoplasia was 2.48 times higher (RR = 2.48, 95% confidence interval: 1.20–5.13) in participants who had beyond 2 years of delayed colonoscopy compared with those with on‐time colonoscopy. Colonoscopy delay did not increase the risk of advanced neoplasia in participants with negative interval FIT results.ConclusionSurveillance colonoscopy can be safely extended beyond 6 months in elevated colorectal cancer risk patients who do not have prior advanced adenoma diagnosis, particularly if interval FIT is negative.
Association between higher variability in kidney function and long‐term mortality
Authors: Ryu Jiwon , Park Yujin , Kim Hye Won , Kim Nak‐Hyun , Kim Su Hwan , Lee Seung Min , Bae Ye Seul , Yoon Hyung‐Jin 
Published: March 2022

AbstractAimWe evaluated whether estimated glomerular filtration rate variability in the general population could be associated with all‐cause mortality.MethodsHealth examination data from 7842 individuals aged >20 years who visited for health check‐ups at least thrice at ≥6‐month intervals between May 1, 1995 and November 30, 2010 were collected. Estimated glomerular filtration rate variability was defined as the coefficient of variation of the estimated glomerular filtration rate, that is, standard deviation/mean value multiplied by 100. The study population was divided into three groups based on the coefficient of variation tertiles, and the mortality risks were compared across groups.ResultsThe mean duration from the final visit to the outcome was 10.3 ± 2.9 years. The mean coefficient of variations of estimated glomerular filtration rate variability from the lowest to the highest variability group were 5.1 ± 1.8%, 9.0 ± 1.0%, and 14.4 ± 3.9%, respectively. There was a 1.3 times higher risk of mortality in the group with the highest variability (hazard ratio: 1.300, 95% confidence interval: 1.013–1.669) after adjustment. The findings were similar in patients with diabetes and those >60 years old (hazard ratio: 1.635, 95% confidence interval: 1.076–2.483; hazard ratio: 1.585, 95% confidence interval: 1.107–2.269).ConclusionHigher estimated glomerular filtration rate variability was associated with increased 10‐year mortality in the general population. This variability was very small, but considering the patients' long‐term prognoses, it was significant.
Free to escape? Economic freedoms, growth and poverty traps
Authors: Saccone Donatella , Migheli Matteo 
Published: March 2022

AbstractNew evidence on the relationships between economic freedoms and poverty traps is provided. Methodologically, a new way to classify countries into clusters is used, which stresses the relative position of economies with respect to each other. The paper investigates whether economic freedoms have any impact on shifts from one cluster to another, towards either better or worse situations. Moreover, it analyzes the contribution of economic freedoms to avoid falling into a poverty trap. The impacts of the five macro‐components of the index are studied separately. The results show that economic freedoms help economies to grow and to avoid poverty traps.
Genomics‐based higher classification of the species‐rich hairstreaks (Lepidoptera: Lycaenidae: Eumaeini)
Authors: Robbins Robert K. , Cong Qian , Zhang Jing , Shen Jinhui , Busby Robert C. , Faynel Christophe , Duarte Marcelo , Martins Ananda R. P. , Prieto Carlos , Lamas Gerardo , Grishin Nick V. 
Published: March 2022

AbstractWe propose a higher classification of the lycaenid hairstreak tribe Eumaeini – one of the youngest and most species‐rich butterfly tribes – based on autosome, Lepidopteran Z sex chromosome and mitochondrial protein‐coding genes. The subtribe Neolycaenina Korb is asynonymof Callophryidina Tutt and subtribe Tmolusina Bálint is asynonymof Strephonotina K. Johnson, Austin, Le Crom, & Salazar. Proposed names are Rhammina Prieto & Busby,new subtribe; Timaetina Busby & Prieto,new subtribe; Atlidina Martins & Duarte,new subtribe; Evenina Faynel & Grishin,new subtribe; Jantheclina Robbins & Faynel,new subtribe; Paiwarriina Lamas & Robbins,new subtribe; Cupatheclina Lamas & Grishin,new subtribe; Parrhasiina Busby & Robbins,new subtribe; Ipideclina Martins & Grishin,new subtribe; and Trichonidina Duarte & Faynel,new subtribe. Phylogenetic results from the autosome and Z sex chromosome analyses are similar. Future analyses of datasets with hundreds of terminal taxa may be more practical time‐wise by focussing on the smaller number of sex chromosome sequences (2.6% of nuclear protein‐coding sequences). The phylogenetic classification and biological summaries for each subtribe suggest that a variety of factors affected Eumaeini diversification. About a dozen kinds of male secondary sexual organs with frequent evolutionary gains and losses occur in Atlidina, Evenina and Jantheclina (141 species combined). Females have been shown to use these organs to discriminate between conspecific and nonconspecific males, facilitating sympatry among close relatives. Eumaeina, Rhammina and Timaetina (140 species combined) are overwhelmingly montane with some evidence for a higher incidence of sympatric diversification. Seven Neotropical lineages in five subtribes invaded the temperate parts of the Nearctic Region with a diversification increase in the Callophryidina (262 species). North AmericanSatyriumandCallophrysthen invaded the Palearctic at least once each, with a major species‐richness increase inSatyrium. The evolution of litter‐feeding detritivores within Calycopidina (172 species) resulted in an increase in diversification rate compared with its flower‐feeding sister lineage. Atlidina, Strephonotina, Parrhasiina and Strymonina (562 species combined) each contain a mixture of genera that specialize on one or two caterpillar food plant families and genera that are polyphagous. These would be appropriate subtribes to assess how the breadth of caterpillar food plants and the frequency of host shifts affected diversification.
Flat Physicalism
Authors: Hemmo Meir , Shenker Orly 
Published: March 2022

AbstractThis paper describes a version of type identity physicalism, which we callFlat Physicalism, and shows how it meets several objections often raised against identity theories. This identity theory is informed by recent results in the conceptual foundations of physics, and in particular clarifies the notion of ‘physical kinds' in light of a conceptual analysis of the paradigmatic case of reducing thermodynamics to statistical mechanics. We show how Flat Physicalism is compatible with the appearance of multiple realisation in the special sciences, and how and in what sense the special sciences laws are autonomous from the laws of physics, despite the full reductive picture of Flat Physicalism. We compare our view with a recent proposal by William Bechtel that accounts for the appearance of levels in mechanistic explanations.
Confirmed non‐invasive prenatal testing for foetal Rh blood group genotyping along with bi‐allelic short insertion/deletion polymorphisms as a positive internal control
Authors: Armstrong‐Fisher Sylvia , Koushki Khadijeh , Mashayekhi Kazem , Urbaniak Stanislaw J. , van der Schoot Ellen , Varzi Ali Mohammad 
Published: March 2022

AbstractBackgroundDetermination of foetus rhesus blood group at risk of hemolytic disease has potential application for early non‐invasive prenatal testing (NIPT). There are several challenges in developing NIPT rhesus blood group genotyping assays by using cell‐free foetal DNA (cff‐DNA) in plasma of RhD‐negative pregnant women. So, the aim of this study was optimization of Real‐time PCR assay for NIPT rhesus genotyping and development of Bi‐allelic short insertion/deletion polymorphisms (INDELs) as internal control to optimise and validate rhesus genotyping based on Real‐time PCR to avoid false or negative results.Material and MethodsNIPT Rhesus genotyping including RHD (exon 7), RHCc, and RHEe genes were performed by TaqMan Real‐time PCR on 104 maternal samples at different gestation ages (12 to ≥40 weeks) from 51 alloimmunized pregnant women. The sensitivity protocol was confirmed with standard DNA samples. Eight selected INDELs were designed and used to detectable cff‐DNA in maternal plasma. INDELs frequency and inheritance were determined on 6 family and 61 unrelated individuals. Finally, multiplex Real‐time PCR was performed for each sample with INDELs pairs and Rh probes.ResultsThe results showed 100% accuracy rhesus typing for RHD, RHC and RHE assays and 95.7% accuracy for RHc. Also, eight selected INDELs as internal control for NIPT were 100% concordance for typed samples.ConclusionThe Real‐time PCR assay is a suitable method with high sensitivity and specificity for rhesus typing as NIPT for prediction of hemolytic disease in foetuses. The INDELs described here are suitable internal control for confirmation of NIPT on cff‐DNA.
Altered voltage‐dependence of slowly activating chloride‐proton antiport by late endosomal ClC‐6 explains distinct neurological disorders
Authors: Zifarelli Giovanni , Pusch Michael , Fong Peying 
Published: March 2022

AbstractClC‐6 is an intracellularly localised member of the CLC family of chloride transport proteins. It presumably functions in the endolysosomal compartment as a chloride‐proton antiporter, despite a paucity of biophysical studies in direct support. Observations of lysosomal storage disease, as well as neurodegenerative disorders, emerge with its disruption by knockout or mutation, respectively. An incomplete understanding of wild‐type ClC‐6 function obscures clear mechanistic insight into disease aetiology. Here, high‐resolution recording protocols that incorporate extreme voltage pulses permit detailed biophysical measurement and analysis of transient capacitive, as well as ionic transport currents. This approach reveals that wild‐type ClC‐6 activation and transport require depolarisation to voltages beyond 140 mV. Mutant Y553C associated with early‐onset neurodegeneration exerts gain‐of‐function by shifting the half‐maximal voltage for activation to less depolarised voltages. Moreover, we show that the E267A proton glutamate mutant conserves transport currents, albeit reduced. Lastly, the positive shift in activation voltage shown by V580M, a mutant identified in a patient with late‐onset lysosomal storage disease, can explain loss‐of‐function leading to disease.Key points
Ionic composition and pH within intracellular compartments, such as endolysosomes, rely on the activity of chloride/proton transporters including ClC‐6.
Distinct CLCN6 mutations were previously found in individuals with neurodegenerative disease, and also putatively associated with neuronal ceroid lipofuscinosis.
Limited knowledge of wild‐type ClC‐6 transport function impedes understanding of mechanisms underlying these conditions.
We resolved transient and transport currents that permit measurement of voltage‐ and pH‐dependences, as well as kinetics, for wild‐type and disease‐associated mutant ClC‐6s.
These findings define wild‐type ClC‐6 function robustly, and reveal how alterations of the slow activation gating of the transporter cause different kinds of neurological diseases.
Dust Acoustic Solitons in Saturn’s Dust-Filled Magnetosphere
Authors: Kopnin S. I. , Shokhrin D. V. , Popel S. I. 
Published: March 2022

Abstract
The possibility is considered of propagation of localized wave structures, such as dust acoustic solitons, in the plasma of the dust-filled Saturn’s magnetosphere, which contains electrons of two sorts (hot and cold) subject to kappa distribution, ions, and charged dust grains. The ranges of possible velocities and amplitudes of the solitons are determined. Soliton solutions for different sizes and concentrations of dust grains in the dust-filled Saturn’s magnetosphere are found.
‘Refbin’ an online platform to extract and classify large-scale information: a pilot study of COVID-19 related papers
Authors: Lunna Shania , Flinn Isabelle , Prytherch James , Torfs-Leibman Camille , Robtoy Sarah , Bansak Matt , Krag David 
Published: March 2022

IntroductionThe number of new biomedical manuscripts published on important topics exceeds the capacity of single persons to read. Integration of literature is an even more elusive task. This article describes a pilot study of a scalable online system to integrate data from 1000 articles on COVID-19.MethodsArticles were imported from PubMed using the query ‘COVID-19’. The full text of articles reporting new data was obtained and the results extracted manually. An online software system was used to enter the results. Similar results were bundled using note fields in parent–child order. Each extracted result was linked to the source article. Each new data entry comprised at least four note fields: (1) result, (2) population or sample, (3) description of the result and (4) topic. Articles underwent iterative rounds of group review over remote sessions.ResultsScreening 4126 COVID-19 articles resulted in a selection of 1000 publications presenting new data. The results were extracted and manually entered in note fields. Integration from multiple publications was achieved by sharing parent note fields by child entries. The total number of extracted primary results was 12 209. The mean number of results per article was 15.1 (SD 12.0). The average number of parent note fields for each result note field was 6.8 (SD 1.4). The total number of all note fields was 28 809. Without sharing of parent note fields, there would have been a total of 94 986 note fields.ConclusionThis pilot study demonstrates the feasibility of a scalable online system to extract results from 1000 manuscripts. Using four types of notes to describe each result provided standardisation of data entry and information integration. There was substantial reduction in complexity and reduction in total note fields by sharing of parent note fields. We conclude that this system provides a method to scale up extraction of information on very large topics.
Listen Carefully protocol: an exploratory case–control study of the association between listening effort and cognitive function
Authors: Feldman Alix , Patou François , Baumann Monika , Stockmarr Anders , Waldemar Gunhild , Maier Anja M , Vogel Asmus 
Published: March 2022

IntroductionA growing body of evidence suggests that hearing loss is a significant and potentially modifiable risk factor for cognitive impairment. Although the mechanisms underlying the associations between cognitive decline and hearing loss are unclear, listening effort has been posited as one of the mechanisms involved with cognitive decline in older age. To date, there has been a lack of research investigating this association, particularly among adults with mild cognitive impairment (MCI).Methods and analysis15–25 cognitively healthy participants and 15–25 patients with MCI (age 40–85 years) will be recruited to participate in an exploratory study investigating the association between cognitive functioning and listening effort. Both behavioural and objective measures of listening effort will be investigated. The sentence-final word identification and recall (SWIR) test will be administered with single talker non-intelligible speech background noise while monitoring pupil dilation. Evaluation of cognitive function will be carried out in a clinical setting using a battery of neuropsychological tests. This study is considered exploratory and proof of concept, with information taken to help decide the validity of larger-scale trials.Ethics and disseminationWritten approval exemption was obtained by the Scientific Ethics Committee in the central region of Denmark (De Videnskabsetiske Komiteer i Region Hovedstaden), reference 19042404, and the project is registered pre-results at clinicaltrials.gov, referenceNCT04593290, Protocol ID 19042404. Study results will be disseminated in peer-reviewed journals and conferences.
Identifying priorities for research on financial risk protection to achieve universal health coverage: a scoping overview of reviews
Authors: Bhatia Dominika , Mishra Sujata , Kirubarajan Abirami , Yanful Bernice , Allin Sara , Di Ruggiero Erica 
Published: March 2022

ObjectivesFinancial risk protection (FRP) is an indicator of the Sustainable Development Goal 3 universal health coverage (UHC) target. We sought to characterise what is known about FRP in the UHC context and to identify evidence gaps to prioritise in future research.DesignScoping overview of reviews using the Arksey & O’Malley and Levac & Colquhoun framework and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews reporting guidelines.Data sourcesMEDLINE, PsycINFO, CINAHL-Plus and PAIS Index were systematically searched for studies published between 1 January 1995 and 20 July 2021.Eligibility criteriaRecords were screened by two independent reviewers in duplicate using the following criteria: (1) literature review; (2) focus on UHC achievement through FRP; (3) English or French language; (4) published after 1995 and (5) peer-reviewed.Data extraction and synthesisTwo reviewers extracted data using a standard form and descriptive content analysis was performed to synthesise findings.Results50 studies were included. Most studies were systematic reviews focusing on low-income and middle-income countries. Study periods spanned 1990 and 2020. While FRP was recognised as a dimension of UHC, it was rarely defined as a concept. Out-of-pocket, catastrophic and impoverishing health expenditures were most commonly used to measure FRP. Pooling arrangements, expansion of insurance coverage and financial incentives were the main interventions for achieving FRP. Evidence gaps pertained to the effectiveness, cost-effectiveness and equity implications of efforts aimed at increasing FRP. Methodological gaps related to trade-offs between single-country and multicountry analyses; lack of process evaluations; inadequate mixed-methods evidence, disaggregated by relevant characteristics; lack of comparable and standardised measurement and short follow-up periods.ConclusionsThis scoping overview of reviews characterised what is known about FRP as a UHC dimension and found evidence gaps related to the effectiveness, cost-effectiveness and equity implications of FRP interventions. Theory-informed mixed-methods research using high-quality, longitudinal and disaggregated data is needed to address these objectives.