bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imaging cerebral arteries tortuosity and velocities by transcranial Doppler ultrasound is a reliable assessment of brain aneurysm in mouse models
Authors: Lebas Héloïse , Boutigny Alexandre , Maupu Clémence , Salfati Jonas , Orset Cyrille , Mazighi Mikael , Bonnin Philippe , Boulaftali Yacine 
Published: January 2022

AbstractBackground and PurposeIntracranial aneurysms (IAs) are common vascular abnormalities of the brain with a prevalence of 3.2% in the general population. In the past few decades, several pathophysiological processes leading to IA rupture were identified, including irregular IA shape, an altered hemodynamic stress within the IA and vessel wall inflammation. The use of preclinical models of IA and imaging tools are paramount to better understand the underlying disease mechanisms. Therefore, there is a need for imaging methods to monitor intracranial aneurysm formation.MethodsWe used two established mouse models of IA and we analyzed the progression of the IA by magnetic resonance imaging (MRI), transcranial Doppler (TCD), and histological studies.ResultsIn both models of IA, we observed by TCD a significant decrease of the blood velocities and wall shear stress of the internal carotid arteries (ICA). We also observed the formation of tortuous arteries in both models which were correlated with the presence of an aneurysm as confirmed by MRI and histological analysis. A high grade of tortuosity has been associated with a significant decrease of the mean blood flow velocities and a greater artery dilation.ConclusionsTCD is robust and easy imaging method to evaluate the progression of IA. The decrease of the blood flow velocities and the tortuosity can be used as reliable readout for IA detection.
The minimizer Jaccard estimator is biased and inconsistent*
Authors: Belbasi Mahdi , Blanca Antonio , Harris Robert S. , Koslicki David , Medvedev Paul 
Published: January 2022

AbstractMotivationSketching is now widely used in bioinformatics to reduce data size and increase data processing speed. Sketching approaches entice with improved scalability but also carry the danger of decreased accuracy and added bias. In this paper, we investigate the minimizer sketch and its use to estimate the Jaccard similarity between two sequences.ResultsWe show that the minimizer Jaccard estimator is biased and inconsistent, which means that the expected difference (i.e., the bias) between the estimator and the true value is not zero, even in the limit as the lengths of the sequences grow. We derive an analytical formula for the bias as a function of how the shared k-mers are laid out along the sequences. We show both theoretically and empirically that there are families of sequences where the bias can be substantial (e.g. the true Jaccard can be more than double the estimate). Finally, we demonstrate that this bias affects the accuracy of the widely used mashmap read mapping tool.AvailabilityScripts to reproduce our experiments are available on GitHub [26].Contactpzm11@psu.edu
Asymmetric Pendrin Homodimer Reveals its Molecular Mechanism as Anion Exchanger
Authors: Liu Qianying , Zhang Xiang , Huang Hui , Chen Yuxin , Wang Fang , Hao Aihua , Zhan Wuqiang , Mao Qiyu , Hu Yuxia , Han Lin , Sun Yifang , Zhang Meng , Liu Zhimin , Li Genglin , Zhang Weijia , Shu Yilai , Sun Lei , Chen Zhenguo 
Published: January 2022

AbstractPendrin SLC26A4 is an anion exchanger expressed in apical membranes of selected epithelia. Pendrin ablation causes Pendred syndrome, a genetic disorder disease associated with sensorineural hearing loss, hypothyroid goiter, and reduced blood pressure. However, its molecular structure has remained unknown limiting our understanding. Here, we determined the structures of mouse pendrin with symmetric and characteristically asymmetric homodimer conformations by cryo-electron microscopy. The asymmetric homodimer consists of an inward-facing protomer and an intermediate-state protomer, representing the coincident uptake and secretion process, and exhibits the unique state of pendrin as an electroneutral exchanger. This previously unrevealed conformation, together with other conformations we captured, provides an inverted alternate-access mechanism for anion exchange. Furthermore, our structural and functional data disclosed the properties of anion exchange cleft and interpreted the important pathogenetic mutations. These investigations shed light on the pendrin exchange mechanism and extend our structure-guided understanding of pathogenetic mutations.One-Sentence SummaryCryo-EM asymmetric Pendrin homodimer cracks its exchange mechanism and guides our understanding for pathogenetic mutations.
3D multi-color far-red single-molecule localization microscopy with probability-based fluorophore classification
Authors: Siemons Marijn E. , Jurriens Daphne , Smith Carlas S. , Kapitein Lukas C. 
Published: January 2022

AbstractSingle-Molecule Localization Microscopy remains limited in its ability for robust and simple multi-color imaging. Whereas the fluorophore Alexa647 is widely used due to its brightness and excellent blinking dynamics, other excellent blinking fluorophores, such as CF660 and CF680, spectrally overlap. Here we present Probability-based Fluorophore Classification, a method to perform multi-color SMLM with Alexa647, CF660 and CF680 that uses statistical decision theory for optimal classification. The emission is split in a short and long wavelength channel to enable classification and localization without a major loss in localization precision. Each emitter is classified using a Generalized Maximum Likelihood Ratio Test using the photon statistics of both channels. This easy-to-adopt approach does not require nanometer channel registration, is able to classify fluorophores with tunable low false positive rates (<0.5%) and optimal true positive rates and outperforms traditional ratiometric spectral de-mixing and Salvaged Fluorescence. We demonstrate its applicability on a variety of samples and targets.
Vexitoxins: a novel class of conotoxin-like venom peptides from predatory gastropods of the genus Vexillum
Authors: Kuznetsova Ksenia G. , Zvonareva Sofia S. , Ziganshin Rustam , Mekhova Elena S. , Dgebuadze Polina , Yen Dinh T.H. , Nguyen Thanh H.T. , Moshkovskii Sergei A. , Fedosov Alexander E. 
Published: January 2022

AbstractVenoms of predatory marine cone snails (the family Conidae, order Neogastropoda) are intensely studied because of the broad range of biomedical applications of the neuropeptides that they contain, conotoxins. Meanwhile anatomy in some other neogastropod lineages strongly suggests that they have evolved similar venoms independently of cone snails, nevertheless their venom composition remains unstudied. Here we focus on the most diversified of these lineages, the genus Vexillum (the family Costellariidae). We have generated comprehensive multi-specimen, multi-tissue RNA-Seq data sets for three Vexillum species, and supported our findings in two species by proteomic profiling. We show that venoms of Vexillum are dominated by highly diversified short cysteine-rich peptides that in many aspects are very similar to conotoxins. Vexitoxins possess the same precursor organization, display overlapping cysteine frameworks and share several common post-translational modifications with conotoxins. Some vexitoxins show detectable sequence similarity to conotoxins, and are predicted to adopt similar domain conformations, including a pharmacologically relevant inhibitory cysteine-know motif (ICK). The tubular gL of Vexillum is a notably more recent evolutionary novelty than the conoidean venom gland. Thus, we hypothesize lower divergence between the toxin genes, and their ‘somatic’ counterparts compared to that in conotoxins, and we find support for this hypothesis in the molecular evolution of the vexitoxin cluster V027. We use this example to discuss how future studies on vexitoxins can inform origin and evolution of conotoxins, and how they may help addressing standing questions in venom evolution.
Real-world monitoring of BNT162b2 vaccine-induced SARS-CoV-2 B and T cell immunity in naive healthcare workers: a prospective single center study
Authors: Calcoen Bas , Callebaut Kim , Vandenbulcke Aline , Callewaert Nico , Bossuyt Xavier , Van Weyenbergh Johan , Maes Piet , Imbrechts Maya , Vercruysse Thomas , Thibaut Hendrik Jan , Zapf Dorinja , Dieckmann Kersten , Vanhoorelbeke Karen , Geukens Nick , De Meyer Simon , Maes Wim 
Published: January 2022

AbstractBackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the ongoing COVID-19 pandemic. To prevent the massive COVID-19 burden, several vaccination campaigns were initiated. We performed a single center observational trial to evaluate adaptive immunity in naive healthcare workers upon BNT162b2 vaccination.MethodsSerological analysis was performed through conventional immunoassays. Antibody functionality was analyzed via in vitro neutralization assays. Circulating receptor-binding domain (RBD) specific B cells were assessed via flowcytometry. The induction of SARS-CoV-2 specific T cells was investigated through interferon-γ release assay combined with flowcytometric profiling of activated CD4 and CD8 T cells.ResultsThree months after vaccination, all but one of the subjects (N = 31) displayed vaccine-induced neutralizing antibodies. In 10 out of 31 subjects, circulating RBD specific B cells were found of which the rate showed moderate correlation to serological parameters. Specific interferon-γ release was present in all subjects and correlated with the significant upregulation of CD69 on CD4+ and CD8+ T cells and CD40L on CD4+ T cells. Interestingly, no relation was found between B and T cell parameters. In addition, one symptomatic breakthrough infection with the SARS-CoV-2 alpha variant of concern was reported.ConclusionThree months post vaccination, both humoral and cellular immune responses are detectable in all but one participant. No correlation was found between the magnitude of both B and T cell responses.
Hospital accreditation systems and salience of organisational tensions
Authors: Robbins Geraldine , Sweeney Breda , Vega Miguel 
Published: January 2022

PurposeThis study examines how an externally imposed management control system (MCS) – hospital accreditation – influences the salience of organisational tensions and consequently attitudes of management towards the system.Design/methodology/approachData are collected using a case study of a large public hospital in Spain. In-depth interviews were conducted with 27 senior and middle managers across different functions. Relying on the organisational dualities classification in the literature, tensions are unpacked and analysed.FindingsEvidence is presented of how hospital accreditation increases the salience of organisational tensions arising from exposition of the organisational dualities of learning, performing, organising and belonging. Salient tensions were evident in the ambivalent attitudes of management towards the hospital accreditation system.Practical implicationsThe role of mandatory external control systems in exposing ambivalence and tensions will be of interest to organisational managers.Originality/valueThe study extends the management control literature by identifying an active role for an external MCS (accreditation) in increasing the salience of organisational tensions and triggering ambivalence. Contrary to the prior literature, the embedding of both poles of an organisational duality into the MCS is not a necessary precondition for increased tension salience. The range of attitudes towards MCSs beyond those specified in the previous literature (positive/negative/neutral) is extended to include ambivalence.
What drives SMEs to adopt e-commerce? The joint role of testosterone and absorptive capacity
Authors: Gu Jiafeng 
Published: January 2022

PurposeThe study aims to investigate the relationship between chief executive officers' (CEOs') prenatal testosterone exposure, absorptive capacity and e-commerce adoption by small and medium-sized enterprises (SMEs).Design/methodology/approachBased on a study of 1,519 SMEs led by a male CEO in China, the impact of entrepreneur's digital ratio on e-commerce adoption is empirically analyzed through a multivariate logistic model.FindingsThe results show that the second-to-fourth digit ratio (2D:4D), a reverse measurement of prenatal testosterone exposure, is negatively correlated with the adoption of e-commerce by SMEs. This evidence suggests that CEOs with high prenatal testosterone exposure have a higher probability of adopting e-commerce. In addition, this research indicates a positive correlation between absorptive capacity, which is defined by market innovation, process innovation and marketing innovation, and the adoption of e-commerce by SMEs.Originality/valueThis research can contribute to the discussion by providing new insights into the antecedents of the adoption of e-commerce in SMEs.
Repair limit policy of aircraft component based on extended uncertain random renewal reward process
Authors: Zhang Chunxiao , Li Xinwang , Liu Xiaona , Li Qiang , Bai Yizhou 
Published: January 2022

PurposeThe purpose of this paper is to focus on an optimizing maintenance policy with repair limit time for a new type of aircraft component, in which the lifetime is assumed to be an uncertain variable due to no historical operation data, and the repair time is a random variable that can be described by the experimental data.Design/methodology/approachTo describe this repair limit time policy over an infinite time horizon, an extended uncertain random renewal reward theorem is firstly proposed based on chance theory, involves uncertain random interarrival times and stochastic rewards. Accordingly, the uncertain random programming model, which minimized the expected maintenance cost rate, is formulated to find the optimal repair limit time.FindingsA numerical example with sensitivity analysis is provided to illustrate the utility of the proposed policy. It provides a useful reference and guidance for aircraft optimization. For maintainers, it plays an important guiding role in engineering practice.Originality/valueThe proposed uncertain random renewal reward process proved useful for the optimization of maintenance strategy with maintenance limited time for a new type of aircraft components, which provides scientific support for aircraft maintenance decision-making for civil aviation enterprises.
How workplace fun promotes informal learning among team members: a cross-level study of the relationship between workplace fun, team climate, workplace friendship, and informal learning
Authors: Lee Amber Yun-Ping , Chang Po-Chien , Chang Heng-Yu 
Published: January 2022

PurposeThe purpose is to examine the cross-level relationship between workplace fun and informal learning with workplace friendship as a mediator and team climate as a moderator.Design/methodology/approachAdopting a two-wave survey procedure, data were collected from 251 employees working across 45 teams of a leading heat transfer manufacturer in Asia. Hierarchical linear modeling was used to test our hypothesized cross-level moderated mediation model.FindingsOut of the three forms of workplace fun, only two – manager support for fun and coworker socializing – supported our hypotheses. Therefore, not all types of workplace fun are equal and one of the key factors through which fun influences informal learning is by maintaining harmonious interpersonal interactions and high relationship quality in teams.Originality/valueBased on social interdependence theory, this study uncovers the cross-level mechanism of how workplace fun affects informal learning. The findings extend existing research on workplace fun by focusing on not only individual factors but also interpersonal and contextual elements. The findings also provide practical implications for managers to understand the possible impact of workplace fun on employees' informal learning.
Let's fight the infodemic: the third-person effect process of misinformation during public health emergencies
Authors: Chen Liang , Fu Lunrui 
Published: January 2022

PurposeDrawing on the third-person effect (TPE) theory and the theory of planned behavior (TPB) as a theoretical framework, the current study aims to explore the cognitive mechanisms behind how third-person perception (TPP) of misinformation about public health emergencies affects intention to engage in corrective actions via attitude, subjective norms and perceived behavioral control.Design/methodology/approachA total of 1,063 participants in China were recruited via a professional survey company (Sojump) to complete an online national survey during the outbreak of coronavirus (COVID-19) in China. Structural equation modeling using Mplus 7.0 was used to address the research hypotheses.FindingsThe results reveal that attention to online information about public health emergencies significantly predicted TPP. In addition, TPP positively influenced attitude and perceived behavioral control, which, in turn, positively encouraged individuals to take corrective actions to debunk online misinformation. However, TPP did not significantly influence subjective norms. A potential explanation is provided in the discussion section.Research limitations/implicationsThe research extends the TPE theory by providing empirical evidence for corrective actions and uncovers the underlying cognitive mechanism behind the TPE by exploring key variables of the TPB as mediating constructs. These are all significant theoretical contributions to the TPE and offer practical contributions to combating online misinformation.Originality/valueThe research extends the TPE theory by providing empirical evidence for a novel behavioral outcome (i.e. corrective actions in response to misinformation) and uncovers the cognitive mechanism underlying the TPE by exploring key variables of the TPB as mediating constructs. These are all significant theoretical contributions to the TPE and offer practical contributions to combating online misinformation.
Total reward system, job satisfaction and employee productivity on company financial performance: evidence from Indonesian Generation Z workers
Authors: Tarigan Josua , Cahya Jordan , Valentine Albert , Hatane Saarce , Jie Ferry 
Published: January 2022

Purpose
The study of monetary and non-monetary rewards from the human resource management (HRM) perspective has rarely been analyzed so far. There has been extensive study on HRM, yet only a few studies explicitly discuss the correlation between the adoption of a total reward system (TRS) and employee-related outcomes. This paper aims to analyze this important issue to provide inputs for organizations to design compensation strategies that will impact on company’s financial performance with employee productivity and job satisfaction as mediating variables specifically for Generation Z.

Design/methodology/approach
A survey was conducted on 40 companies operating in the service sector in Indonesia. The authors captured the overall TRS result on three outcome variables (i.e. job satisfaction, employee productivity and financial performance). Furthermore, the authors examine the impact of TRS (base pay, training and development and positive work environment) on Generation Z workers. Structural equation modeling analysis was conducted on 40 Indonesian, service industry, listed firms with an average of 6 representatives per firm.

Findings
The results validate that the adoption of a TRS exerts a positive influence on the aforementioned outcomes. However, the interesting fact about the finding is that Generation Z cannot be easily satisfied with only monetary incentives; their preference has shifted from monetary concerns to self-capability.

Practical implications
The empirical result suggests that TRS serves as a tool in encouraging employees and boosting productivity. Accordingly, firms should incorporate TRS practices to enhance job satisfaction and productivity, as well as sustain the relationship with stakeholders. Subsequently, management should also be concerned with maintaining good employee productivity to improve a company’s financial performance by supervising and monitoring company operations, as well as ensuring the fulfillment of the stakeholder’s interests.

Originality/value
This paper provides original insights into the complex relationship between TRS and the aforementioned outcomes, such as job satisfaction, employee productivity and financial performance.
Are extracurricular activities stress busters to enhance students’ well-being and academic performance? Evidence from a natural experiment
Authors: Mukesh Hasirumane Venkatesh , Acharya Vrinda , Pillai Rajasekharan 
Published: January 2022

PurposeThe stress-coping model is extensively studied in the academic context. Past studies have primarily focused on different coping strategies adopted by students to overcome academic stress. However, an important question, how to equip students to cope with stress, was ignored. Drawing on stress-coping theory and the extracurricular activity (ECA) literature, the current study investigates the intervention of ECA participation on students’ coping, academic performance, and well-being in a natural setting.Design/methodology/approachThe study follows a “cross-sectional post-test only quasi-experimental design” using a natural experimental setting.FindingsThe findings indicate that participation in ECA has a significant influence on academic outcomes. Different types of ECA participation influence well-being, whereas time spent on ECA positively affects academic performance. Further, the findings also indicate that involvement in ECA moderates the relationship between academic stress and coping.Practical implicationsThe study results have practical implications for designing interventional ECA to enhance students’ academic outcomes and well-being.Originality/valueThe study indicates the effectiveness of ECA participation in dealing with academic stress and the development of constructive coping strategies. Hence, the authors advise the academic administrators to integrate ECA in the academic setting.
Survival strategies of SMEs amidst the COVID-19 pandemic: application of SEM and fsQCA
Authors: Rahman Muhammad Sabbir , AbdelFattah Fadi AbdelMuniem , Bag Surajit , Gani Mohammad Osman 
Published: January 2022

PurposeAs a global pandemic, the COVID-19 crisis has profoundly affected the development of local firms, threatening the survival of small and medium enterprises (SMEs). This study aims to present an integrated framework by investigating the impact of strategic tools (i.e. firms’ capability of business agility, marketing operational efficiency, optimisation of innovation capability [OIC], managing employees’ satisfaction and rethinking customers’ experience) on the survival strategies of SMEs amidst the COVID-19 pandemic.Design/methodology/approachThe current study used data from managers of SMEs and conducted an asymmetrical analysis (i.e. structural equation modelling [SEM]) to investigate the factors influencing the survival strategies of SMEs amidst the COVID-19 pandemic. This study also applied an asymmetrical approach (i.e. fuzzy sets qualitative comparative analysis-fsQCA) to explore the causal recipes and analysis of the necessary conditions to identify the factors required to achieve the expected outcome.FindingsResults from SEM support all hypotheses. Results from fsQCA with the same data set show that firms’ business agility and OIC are necessary conditions for SMEs’ survival strategies. The result from fsQCA also reveals multiple sufficient conditions to succeed SMEs’ survival strategies amidst the COVID-19 pandemic.Practical implicationsFindings prescribe how SMEs adapt to this vulnerable business condition by applying the strategic tools and recipes suggested for survival.Originality/valueThis research applied an innovative analysis to reveal necessary and sufficient conditions that conventional methods such as SEM have limited power. This pioneering research in the context of the COVID-19 pandemic is considered novel in terms of the prescriptive strategic recipes offered to SMEs to adapt to and survive in the crisis caused by COVID-19.