bluuebunny/crossref_metadata_2025_split huggingface dataset:
UM-164: A c-Src/p38 Kinase Inhibitor inhibits proliferationand induces apoptosis in human glioblastoma cells
Authors: YANG KUN , XIAO MEI-XIAN , TANG XIANG‑JUN , SHAN QIANG , CHEN QIAN-XUE , ZHANG HUI-KAI 
Published: May 2022

Abstract
Glioblastoma is the most common and aggressive primary brain tumor in adults, which
has high mortality and morbidity rates, and short survival time. here we tested a novel c-Src/p38 kinase inhibitor UM-164 in human glioblastoma cells in U87 and U251 cells. CCK-8 assay, EDU staining, colony formation, wound healing, cell cycle distribution analysis, migration and transwell invasion assays showed that UM-164 suppressed the proliferation and dose‑ and time‑dependent cytotoxicity in and exhibited a marked suppression after treating the U87 and U251 cells with UM-164. Flow cytometry, TUNNEL assays, immunofluorescent staining and western blotting test which tested the expression levels of BCL-2, BCL-XL, BAX, Cleaved-caspas-3, Cyclin D1, Cyclin E, MMP-2, MMP-9, P38, P-P38, EGFR, P-EGFR(Tyr1068), AKT, P-AKT, P42 and P-P42 were changed after um-164 treatment have changed and exhibited a strong inhibition for the proliferation and induces apoptosis. taken together, our findings indicate that um-164 exerts an antitumor effect by inhibiting proliferation and inducing apoptosis in human glioma cells. as for the present study provides evidence supportive of further development of multitarget kinase inhibitor UM-164 for adjuvant therapy and give a hope in GBM patients.
Mixed Organic and Inorganic Amendments Enhance Soil Microbial Interactions and environmental stress resistance of Tibetan Barley on Plateau Farmland
Authors: Guan Xiangyu , Cheng Zhen , Li Yiqiang , Zhao Ruoyu , Guo Zining , Zhao Tingting , Huang Liying , Qiu Cheng , Chen Liang , Shi Wenyu , Jin Song , Wang Jinfeng 
Published: May 2022

Abstract
BackgroundSufficient crop yield while maintaining soil health and sustainable agricultural development is a global objective, serving a special challenge to certain climate-sensitive plateau areas. Despite conducting trails on a variety of soil amendments in plateau areas, systematic research is lacking regarding the influences of organic and inorganic amendments on soil quality, particularly soil microbiome. To our knowledge, this was the first study that compared the effects of inorganic, organic, and mixed amendments on typical plateau crop hulless barley (Hordeum vulgare L. var. nudum; also known as “Qingke” in Chinese) in Tibet over the course of tillering, jointing, and ripening. ResultsMicrobial communities and their responses to amendments, soil properties, Tibetan hulless barley growth, yield, and their indicator microbial species were investigated. Results indicated that mixed organic and inorganic amendments promoted the abundance of rhizosphere microorganisms, enhancing the rhizosphere root-microbes interactions and resistance to pathogenic bacteria and environmental stresses. Additionally, values of total organic carbon (TOC) and carbon to nitrogen (C/N) ratio and the hullesss barley yield were the highest when mixed organic and inorganic amendments were applied. The rhizosphere microbiome demonstrated its unique adaptation to the plateau environment in carbon, nitrogen, sulfur, and phosphorus cycles, as well as resistance to environmental stresses. The 23 genes involved in plant growth and environmental adaptation in the mixed amendments treatments were significantly higher than other treatments. ConclusionsFindings from this study suggest that the mixed organic/inorganic amendments can help establish a healthy microbiome and increase soil quality while achieving sufficient hulless barley yields in Tibet and presumably other similar geographic areas of high altitude.
TSPO PET Signal is Associated with Survival in Recurrent Gliomas
Authors: Quach Stefanie , Holzgreve Adrien , Kaiser Lena , Unterrainer Marcus , Dekorsy Franziska J. , Nelwan Debie V. , Bartos Laura M. , Kirchleitner Sabrina V. , Weller Jonathan , Weidner Lorraine , Niyazi Maximilian , Ruf Viktoria C. , Herms Jochen , Stöcklein Sophia , Wetzel Christian , Riemenschneider Markus J. , von Baumgarten Louisa , Thon Niklas , Brendel Matthias , Rupprecht Rainer , Bartenstein Peter , Tonn Joerg-Christian , Albert Nathalie Lisa 
Published: May 2022

Abstract
Purpose
Glioma patients, especially recurrent glioma, suffer from a poor prognosis. While advances to classify glioma on a molecular level improved prognostication at initial diagnosis, markers to prognosticate survival in the recurrent situation are still needed. As 18 kDa translocator protein (TSPO) was previously reported to be associated with aggressive histopathological glioma features, we correlated the TSPO positron emission tomography (PET) signal in a large cohort of recurrent glioma patients with their clinical outcome.
Methods
In patients with TSPO PET at glioma recurrence, TSPO PET parameters (e.g. SUVmax) as well as other imaging features (e.g. MRI volume, [18F]FET PET parameters when available) were evaluated together with patient characteristics (age, sex, Karnofsky-Performance score) and neuropathological features (e.g. WHO grade, IDH-mutation status). Uni- and multivariate Cox regression and Kaplan-Meier survival analyses were performed to identify prognostic factors for post-recurrence survival (PRS) and time to treatment failure (TTF).
Results
88 consecutive patients were evaluated. TSPO tracer uptake correlated with tumor grade at recurrence (p < 0.05), with no significant differences in IDH-wildtype versus IDH-mutant tumors. Within the subgroup of IDH-mutant glioma (n = 46), patients with low SUVmax (median split, ≤ 1.60) had a significantly longer PRS (median 41.6 vs. 25.3 months, p = 0.031) and TTF (32.2 vs 8.7 months, p = 0.001). Also among IDH-wildtype tumors (n = 42), patients with low SUVmax (≤ 1.89) had a significantly longer PRS (median not reached vs 8.2 months, p = 0.002). SUVmax remained an independent prognostic factor for PRS in the multivariate analysis including CNS WHO grade, IDH status and age. Tumor volume defined by [18F]FET PET or contrast-enhanced MRI correlated weakly with TSPO tracer uptake. Treatment regimen did not differ among the median split subgroups.
Conclusion
Our data suggest that TSPO PET can help to prognosticate recurrent glioma patients even among homogeneous molecular subgroups and may therefore serve as valuable non-invasive biomarker for individualized patient management.
Clinicopathologic characteristics of thymic clear cell carcinoma: a case report with literature review
Authors: Zhao Zuxuan , Zeng Qingpeng , Li Jiangtao , Zheng Shan 
Published: May 2022

Abstract
Background: Thymic clear cell carcinoma is a rare mediastinal neoplasm, with only 25 reported cases to date. We report a case of a 45-year-old man with thymic clear cell carcinoma. We think imaging and laboratory tests may be helpful for differential diagnosis.Case presentation: A 45-year-old male was admitted to a local hospital for chest distress with cardiopalmus. CT showed a mediastinal mass. Laboratory examination results were all in the normal range. Histologically, the tumor cells had a clear cytoplasm, and immunohistochemically, the tumor cells were positive for epithelial markers. We performed abdominal and pelvic CT and further examined serum levels of thyroxine, parathyroid hormone and AFP postoperatively, which were normal. The patient received postoperative radiotherapy and chemotherapy, and CT showed left adrenal metastasis at 20 months after surgery.Conclusion: Thymic clear cell carcinoma is a rare malignant neoplasm. Adrenal metastasis can occur. Patients undergo thymectomy with chemotherapy or with radiotherapy have better outcoming. Metastasis, direct invasion of parathyroid carcinoma and other primary tumors in the mediastinum should be excluded. Immunohistochemical markers, imaging and laboratory examination can help to exclude metastasis.
A Novel N7-Methylguanosine-Related lncRNA Signature Predicts the Prognosis of Colon Adenocarcinoma Patients
Authors: Sun Guowei , Fu Yayan , Shao Wenzhe , Zhang Cangyuan , Long Ziyang , Bao Li , Sun Biao , Wang Wei , Wang Liuhua , Ren Jun , Sun Qiannan , Tang Dong , Wang Daorong 
Published: May 2022

Abstract
Background: The most common subtype of colorectal cancer is colon adenocarcinoma (COAD). However, few studies have investigated the predictive value of N7-methylguanosine(m7G)-related long-noncoding RNAs (lncRNAs) in COAD. We aim to use m7G-related lncRNAs to construct a prognostic model for COAD.Methods: RNA-seq data and the corresponding clinical and prognostic Data were downloaded from The Cancer Genome Atlas (TCGA) database.M7G-related lncRNAs with prognostic values were identified using the univariate and multivariate Cox regression. Based on Kaplan-Meier curves, we compared the survival rates in the high-risk and low-risk groups. The functional enrichment of m7G-related lncRNAs with a prognostic value was interpreted using gene set enrichment analysis (GSEA). Finally, we studied the relationship between predictive signature and treatment response in patients with COAD.Results: Six m7G-related lncRNAs (LINC01063, ARRDC1-AS1, AL354993.2, ZEB1-AS1, SNHG16, LINC02474) were identified to establish a signature. The m7G-related lncRNA signature may be able to predict COAD patients' prognosis independently. The OS times of patients in the high-risk group were shorter than those in the low-risk group. With an area under the receiver operating characteristic curve of 0.742, the m7G-related lncRNA prognostic signature had a better prognostic prediction than clinicopathological variables. The high-risk group has the most tumor-related pathways, according to GSEA. The prognostic characteristic was linked to the immunological state of COAD patients, according to GSEA. The conventional chemotherapy medicines axitinib, cytarabine, sorafenib, parthenolide and vorinostat were more sensitive in high-risk individuals.Conclusion: Our study develops a COAD risk model consisting of six lncRNAs related to m7G that have independent prognostic values. Clinically, the lncRNA signature could improve prediction of outcomes for patients with COAD and, with further prospective validation, could help guide tailored therapy for COAD patients
The Pyroptosis-Related Signature Predicts Prognosis and Indicates New Treatment Targets in Pancreatic Cancer
Authors: Zhao Zhenyu , Yan Hai , Li Xiao , Yu Yanling , Wang Chunyan 
Published: May 2022

Abstract
Purpose
Pancreatic adenocarcinoma (PAAD) is a malignant tumor leading cause of cancer death worldwide. Pyroptosis is a recently identified inflammatory cell death that is associated with tumor progression, prognosis, and treatment response. Our study investigated the potential roles of pyroptosis-related genes (PRGs) in the tumor microenvironment (TME).
Methods
We explored 48 PRGs in 350 PAAD samples from genetic and transcriptional fields. Immunocyte subsets in three subgroups were investigated and we found that B-PRGcluster was correlated with PAAD progression and TME cell- infiltrating characteristics. Then, we established a highly accurate nomogram for improving the clinical applicability of the PRG_score. Moreover, we evaluated chemotherapeutic agents used for PAAD to assess the susceptibility of patients in low-risk and high-risk groups to these agents.
Results
Significant differences in the genetic landscape and expression levels of PRGs between PAAD and normal samples indicated a potential role for PRGs in the development of PAAD. The prognosis of PAAD patient was significantly correlated with the expression levels of PRG. The infiltration immune cells in B_PRGcluster was identified which play the role of PRG in the TME of PAAD. The PFS of 1,2 and 3 years had higher accuracy, and TP53 was the second most frequently mutated gene after KARS in high-risk and low-risk groups classified by PRG-score. The rise of Risk Score indicated that the number of patients in Dead state was significantly increased, and the gene expression showed a positive correlation with Risk Score. The correlation between Tumor Burden Mutation and risk score showed a significant positive correlation.
Conclusion
Our investigation of PRGs in PAAD revealed their potential roles in the TME, clinicopathological features, and prognosis. These findings highlight a new insight in the understanding of PRGs in PAAD and offer a novel approach to immunotherapy strategies.
Genomic Surveillance of SARS-COV-2 reveals diverse circulating variant lineages in Nairobi and Kiambu County, Kenya
Authors: Kuja Josiah O. , Kanoi Bernard N. , Shiluli Clement , Maina Michael , Waweru Harrison , Gethii Kimita , Mungai Mary , Masika Moses , Anzala Omu , Mwau Matilu , Clark Taane G. , Waitumbi John , Gitaka Jesse 
Published: May 2022

Abstract
Genomic surveillance and identification of SARS-CoV-2 outbreaks are important in understanding the genetic diversity, phylogeny, and lineages of SAR-CoV-2. Genomic surveillance provides insights into circulating infections, and insights into the robustness and design of vaccines and other infection control approaches. We sequenced 56 SARS-CoV-2 isolates from a Kenyan clinical population, of which 52 passed the Ultrafast sample Placement on the existing tRE for the phylo-genome-temporal analyses across two regions in Kenya (Nairobi and Kiambu County). B.1.1.7 (Alpha; n = 32, 61.5%) and B.1 (n = 9, 17.3%) lineages were the most predominant variant with a wide-range of Ct values (5–31) and variant mutations across the two regions. Lineages B.1.617.2, B.1.1, A.23.1, A.2.5.1, B.1.596, A, and B.1.405 were also detected across the sampling sites within the target population. The lineages and genetic isolates were traced back to China (A), Costa Rica (A.2.5.1), Europe (B.1, B.1.1, A.23.1), USA (B.1.405, B.1.596), South Africa (B.1.617.2), and United Kingdom (B.1.1.7), indicating multiple introduction events. There were, however, no genetic isolates associated with the omicron (B.1.1.529) variant of concern that is less severe than the previous variants.
Highly efficient transgene-free plant genome editing in tobacco using an optimized CRISPR/Cas9 system, pOREU3TR
Authors: Zhang Jianduo , Xing Jiaxin , Mi Qili , Yang Wenwu , Xiang Haiying , Xu Li , Zeng Wanli , Wang Jin , Deng Lele , Jiang Jiarui , Yang Guangyu , Gao Qian , Li Xuemei 
Published: May 2022

Abstract
Background: CRISPR-Cas9 Genome-editing technology has revolutionized the plant science and hold enormous promise in crop improvement. When performing the gene editing system for crops, it is crucial to eliminate of the Cas9/sgRNA T-DNA cassette in the T1 generation. Results: Here, we firstly validated that incorporating an OsU3-tRNA promoter combination in the CRISPR/Cas9 system contributed to the highest mutagenesis efficiency that increased sgRNA expression levels over the AtU6-tRNA and AtU6 promoters by editing the NtPDS gene (Ntab0595110) in tobacco. Then we optimized the existing tobacco CRISPR/Cas9 system by using the OsU3-tRNA promoter combination instead of AtU6, and fusing an AtUb10-Ros1 expression cassette in T-DNA for monitoring the transgene events. The new vector was named as pOREU3TR. As expected, 52 transgene-free and homozygous gene-edited green plants were effectively screened at T1 generation by editing the NtLHT1 gene (Ntab0818090) in tobacco, and the contents of most free amino acids in the T2 mutants ntlht1 leaves were detected significantly different from those in the wild type leaves, demonstrating the highly efficient of the system.Conclusions: This OsU3-tRNA-sgRNA/AtUb10-Ros1 system provides essential improvements to increase the efficiency of plant genome editing.
Adropin is involved in the protective effect of ellagic acid against high-glucose induced injury in human umbilical venous endothelial Cells
Authors: Sheikh Somayeh , Dehghani Hesam , Kazerani Hamid Reza 
Published: May 2022

Abstract
Background: There is escalating evidence suggesting the beneficial effects of ellagic acid (EA) on the cardiovascular system. The present study aimed to investigate the protective effect of EA in human umbilical vein endothelial cells (HUVECs) against high glucose (HG)- induced endothelial dysfunction and the potential roles of adropin and NO in this regard.Methods and Results: The experimental groups consisted of normal and HG (30 mM, 48 h) treated HUVECs incubated without or with 5 or 10 μM of EA (6 groups of at least 6 replicates, each). The cell count and metabolism were studied. Moreover, the markers of redox state including malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase enzymes, as well as ferric reducing antioxidant power (FRAP) were assayed. The levels of adropin and eNOS gene expression were also studied using qPCR. A high concentration of glucose reduced cell count and caused lipid peroxidation, reduced antioxidant capacity of the cells, decreased NO levels, and downregulated the expression of NOS3 (encoding eNOS) and ENHO (encoding Adropin) genes. Ellagic acid reversed all these effects.Conclusions: These results suggest a significant protective effect for EA against HG-induced injury in HUVECs. The improved redox state and upregulation of NOS3 and ENHO genes seem to play critical roles in this regard.
Predicting hypogonadotropic hypogonadism persistence in male macroprolactinoma
Authors: Rudman Yaron , Duskin-Bitan Hadar , Masri-Iraqi Hiba , Akirov Amit , Shimon Ilan 
Published: May 2022

Abstract
Purpose:To study the baseline characteristics predicting hypogonadotropic hypogonadism (HH) persistence in men with macroprolactinoma that achieved prolactin normalization.Design:Retrospective cohort study.Methods:Male patients diagnosed with macroprolactinoma and HH that received cabergoline treatment with subsequent prolactin normalization were included: men that achieved eugonadism, and men that remained hypogonadal. Patient’s demographic, clinical and biochemical parameters, sellar imaging and visual fields tests were obtained. Univariate and multivariate models were used to identify predictors of HH persistence.Results:Fifty-eight male patients (age, 49.2±12.6 years) with a median baseline prolactin of 1154 ng/mL (IQR, 478-2763 ng/mL) and adenoma (maximal) diameter of 25.9±14.8 mm were followed for a median of 5.6 years (IQR, 3.0-10.7). Twelve men (21%) suffered from HH persistence at the end of follow-up and 46 men achieved eugonadism. Forty-two out of 46 men (91%) accomplished eugonadism within the first year following prolactin normalization.In a multivariate logistic regression model, hypopituitarism (OR=10.1; 95% CI 1.10 – 101.94), visual field defect (OR=9.9; 95% CI 1.07 – 92.33), and low baseline testosterone levels (OR=0.5; 95% CI 0.29 – 0.93) were independent predictors of HH persistence.Conclusion:In our cohort of men with macroprolactinoma that reached prolactin normalization with cabergoline treatment, 21% had HH persistence. Pituitary hormone deficiency, visual field defects, and low baseline testosterone levels were independently associated with HH persistence. 91% of men achieved eugonadism within the first year following prolactin normalization. These findings may support informed clinical decision-making regarding the initiation of testosterone replacement in men with macroprolactinomas.
Prevalence and economic importance of bovine hydatidosis in animal slaughtered in Dalomana municipal abattoir Southeastern, Ethiopia
Authors: Jilo Sufian Abdo , Abadura Sadik zakir , shibeshi Nigatu Taddese , Hussein Johar Aliye , Nair Sureshkumar p. , Gelchu Aliyi Adem 
Published: May 2022

Abstract
A cross-sectional study was conducted on bovine hydatidosis from May 2018 to November 2020 with the aims of finding its incidence and economic losses in bovine slaughtered for human consumption in Dalomana municipality abattoir. Usual ante mortem and post-mortem inspection was carried out on overall of 430 selected slaughtered cattle. From this total inspected, 82(19.1%) of them had harboured hydatid cyst (s) in one or more of their organ’s. Age and body condition as a risk factor were statistically significant as older zebus and medium were more infected (P < 0.05, χ2 = 14.597 and p value < 0.05 χ2 = 9.68) respectively. Similarly, the geographical origin of the animals had significant contribution on hyadatid cyst distribution (P < 0.05, χ2 = 12.724). Sex was statistically non-significant (P > 0.05, χ2 = 0.74) and have no difference.The single and multiple organs were infected by hydatid cysts. From the total of 282 hydatid cysts collected and characterized lung and liver took more percentage in harboring cysts, from this 137(48.58%) and 126(44.68%) cysts were observed in lung and liver respectively and 19(6.74%) are observed in other organ, such as spleen kidney, and heart. The study indicated that annual economic failure due to direct organ condemnation and indirect carcass weight loss from the disease in Dalomana was estimated to be 105769.657 ETB. Hence, bovine hydatidosis is significantly a common disease in cattle, with severe public health fears and leads considerable observable and undetectable losses in bovine in Dalomana and its surroundings.
Characterization and Prediction of Dengue Virus targeting peptides based on three class of descriptors using k-NN and Random Forest algorithm.
Authors: Elumalai Elakkiya , Muthuvel Suresh Kumar 
Published: May 2022

Abstract

Dengue virus peptides are emerging as potential therapeutics for dengue infection. Due to the important role of dengue peptides in curbing dengue infection, their identification has proven crucial in terms of infection biology. To calculate differences between amino acids and physiochemical attributes, statistical tests and F-scores were used in this work. In this study, we presented the first evidence, to our knowledge, for the relationship between dengue virus inhibiting and non-inhibiting peptides with amino acid use and biological properties. We found that the frequency of Glycine (G), Phenylalanine (F), and Tryptophan (W) was significantly higher in dengue virus inhibitory peptides. Similarly, aromatic amino acids in non-inhibiting peptides were found to be less than 5%. The distribution of solvent accessible residues in non-inhibiting peptides was found to be less as compared to inhibiting peptides. The alpha helices and beta sheets in dengue virus inhibiting peptides are equally distributed but in non-inhibiting peptides, the proportion of beta sheets is more as compared to alpha helices. We have used 8 machine learning algorithms to predict dengue peptides. Here we have used three class of descriptors namely Amino acid composition (AAC), grouped amino acid composition, transition and distribution (GAAC) and Composition, transition and distribution features (CTDC). We have compared all 8 machine learning models for the three classes. The machine learning algorithm were, Random forest (RF), Multi-layer perceptron (MLP), Support Vector Machine (SVM), Logistic regression, K-Nearest Neighbour (k-NN), Naivebayes, Adaboost and Bagging. The best model was reposted as AAC_k-NN_model, accuracy was 90.47. Subsequently, other four best model were AAC_SVM_model, CTDC_SVM_model, AAC_RF_model and GAAC_RF_model. In all the three class, k-NN and RF models were found to be the best classifier. Our classifier produced superior predicting outcomes when compared to previously developed algorithms. In conclusion, we looked at the differences in amino acids and physiochemical properties between dengue viral peptides, using the grouped amino acid composition to build a classifier that predicts these dengue virus inhibitory peptide.