bluuebunny/crossref_metadata_2025_split huggingface dataset:
NIR laser-activated polydopamine-coated Fe3O4 nanoparticles used as a novel precise photothermal insecticide
Authors: Wang Haijun , Zhang Qian , Zhang Xinyu , Zhang Kexin , Zhao Xichen , Zhang Ruiling , Zhang Zhong 
Published: February 2022

Abstract
Chemical pesticides are widely used in prevention and control of pests. However, low insecticidal efficacy, high environmental risks and pesticidal resistance severely constrain further applications. In this study, a near-infrared (NIR) laser-activated pesticide-free Fe3O4@PDA nanoplatform with a low production cost was constructed for precise photothermal killing of housefly larvae. The polydopamine (PDA) surface-functionalized Fe3O4 nanoparticles (Fe3O4@PDA NPs) were synthesized by simply mixing dopamine with Fe3O4 NPs under aerobic alkaline conditions. These Fe3O4@PDA NPs exhibited superb photothermal performance with near-infrared (NIR) laser irradiation. Larval uptake results revealed greater in vivo uptake of Fe3O4@PDA NPs than bare Fe3O4 NPs when housefly larvae were fed a NP-treated diet. These NPs exhibited favorable biocompatibility and showed no obvious influence on larval development and intestinal microbial population. However, when irradiated with a NIR laser, the NPs efficiently induced death of housefly larvae with high temperature (Tmax > 50 ℃) damage to the intestinal wall and subsequent leakage of intestinal bacteria into the hemocoel. Furthermore, uneaten NPs can be collected by magnetic absorption and show excellent thermal stability, which allowed recycling of these environmentally friendly nanoparticles. The Fe3O4-based nanoparticles described in the present study integrate the independence of pesticides, pronounced photothermal control of pests, and environmental friendliness, making this nanoplatform promising for controlling vector pests and diseases.
Rational discovery of small molecule inhibitor targeting invasion and tumor growth
Authors: Kumar Karthiga Santhana , Brunner Cyrill , Schuster Matthias , Kopp Levi , Gries Alexandre , Yan Shen , Jurt Simon , Moehle Kerstin , Bruns Dominique , Grotzer Michael , Zerbe Oliver , Schneider Gisbert , Baumgartner Martin 
Published: February 2022

Abstract
Rational targeting of proteins involved in controlling cancer cell behavior with small bioactive compounds can accelerate anti-cancer drug discovery. We report the identification of a new small molecule compound inhibitor of the FGFR adaptor protein FRS2. Pharmacophore-based computational screening combined with functional, biophysical, and structural binding analyses, led to the identification of low-molecular weight ligands that interact with the PTB domain of FRS2. By assessing the compounds’ anti-invasion activity in human FGFR-driven cancer cell models, three chemically distinct bioactive molecules were shortlisted for further analysis. A lead compound was selected that specifically repressed FGFR-driven MAPK activation and matrix invasion and displayed on-target activity in cells. Proteome-wide off-target activity of the primary lead was determined and functional in vivo efficacy in an FGFR driven ovarian cancer model confirmed. We propose inhibition of FRS2 by a small molecular PTB domain ligand as a strategy to repress FGF signaling in FGFR-driven human cancers.
Neurologic and neuropsychiatric symptoms in long-COVID 19 syndrome: a systematic review
Authors: Junior Giovani Zocche , Siqueira Lia Fonseca , Loro Leonardo Bongiovani , Fortes Felipe Valles , Zanirati Gabriele , Marinowic Daniel , Costa Jaderson Costa da 
Published: February 2022

Abstract
Covid-19 pandemic globally impaired health care, basic assistance, and quality of life, even for those who recovered after SARS-CoV-2 infection. New questions arise regarding post infection and prolonged or new symptoms. This broad concept of COVID-19 long defined as symptoms occurring after infection is very relevant for health care taken together with patient complaints related to new emerging variants and prior infections. Therefore, establishing the relationship between neurological symptoms and COVID-19 is very important. For this review, we considered only neurologic or neuropsychiatric findings after a positive SARS-CoV-2 serological test and COVID-19 long symptoms after a negative PCR test, which resulted in 42 studies after exclusions. Our results demonstrated that the most relevant and frequent neurological complaints reported was fatigue, followed by attention or memory difficulties, including brain fog and smell and taste disturbances. This systematic literature review of long COVID-19 aims to enhance the current knowledge regarding symptoms after infection and to discuss long-term COVID-19 concept, given the possibility of new or persistent symptoms.
Bayesian method for estimating Weibull parameters for wind resource assessment in the Equatorial region: a comparison between two-parameter and three-parameter Weibull distributions
Authors: Khan M. G. M. , Ahmed M. Rafiuddin 
Published: February 2022

Abstract
The two-parameter Weibull distribution has garnered much attention in the assessment of windenergy potential. The estimation of the shape and scale parameters of the distribution has broughtforth a successful tool for the wind energy industry. However, it may be inappropriate to use thetwo-parameter Weibull distribution to assess energy at every location, especially at sites wherelow wind speeds are frequent, such as the Equatorial region. In this work, a robust technique inwind resource assessment using a Bayesian approach for estimating Weibull parameters is firstproposed. Secondly, the wind resource assessment techniques using a two-parameter Weibulldistribution and a three-parameter Weibull distribution which is a generalized form of twoparameterWeibull distribution are compared. Simulation studies confirm that the Bayesianapproach seems a more robust technique for accurate estimation of Weibull parameters. Theresearch is conducted using data from seven sites in Equatorial region from 1o N of Equator to 19oSouth of Equator. Results reveal that a three-parameter Weibull distribution with non-zero shiftparameter is a better fit for wind data having a higher percentage of low wind speeds (0-1 m/s) andlow skewness. However, wind data with a smaller percentage of low wind speeds and highskewness showed better results with a two-parameter distribution that is a special case of threeparameterWeibull distribution with zero shift parameter. The results also demonstrate that theproposed Bayesian approach and application of a three-parameter Weibull distribution areextremely useful in accurate estimate of wind power and annual energy production.
Economic Burden Attributable to Healthcare-Associated Infections in Tertiary Public Hospitals of Central China: A Multi-center Case-control Study
Authors: Li Peng , Li Yan , Zhang Youjian , Bao Junzhe , Yuan Ruixia , Lan Hongwen , Sun Mingjie 
Published: February 2022

Abstract
Background
Healthcare-associated infection (HAI) is a major cause of morbidity, mortality and cost, which vary widely by region and hospital. We aimed to calculate losses attributable to HAI in central China.
Methods
We performed a three-stage random sampling and employed a 1:1 matched case-control study. The direct cost attributable to HAIs was estimated and compared among different types of HAIs by employing a subgroup analysis.
Results
A total of 2976 patients in 10 hospitals were enrolled, and the incidence rate of HAI (range, 0.88%-4.15%) was significantly, but negatively associated with the cost per 1000 beds of its prevention (range, $24929.76-$53146.41; r=-0.76). The per capita economic loss attributable to HAIs was $2047.07 (interquartile range, $327.63-$6429.17), mainly from the pharmaceutical cost (median, $1044.39). The HAIs, which occurred in patients with commercial medical insurance, affected the hematologic system and caused by A. baumannii, contributed most to the losses (median, $3881.55, $4734.20 and $9882.75, respectively), and a significant correlation existed between the economic loss and age (r=0.26). Furthermore, the economic losses attributable to device-associated infections and hospital-acquired multi-drug resistant bacteria were 2 to 4 times those of the controls.
Conclusion
The economic burden attributable to HAI in central China is heavy, and opportunities for easing this burden exist in several areas, including that strengthening antibiotic stewardship and practicing effective bundle of HAI prevention for patients carrying high risk factors, for example, elders or those with catheterizations in healthcare institutions, and accelerating the medical insurance payment system reform based on Diagnosis Related Groups by policy-making departments.
Analysis of the accuracy of a dynamic navigation system when performing dental implant surgery with transcrestal sinus floor elevation using a piezoelectric technique: a retrospective study
Authors: Wu Bin-Zhang , Ma Fei-Fei , Sun Feng 
Published: February 2022

Abstract
Background To evaluate the accuracy associated with the use of a dynamic navigation system when conducting posterior maxilla implant surgery with transcrestal sinus floor elevation (TSFE) a piezoelectric technique.Methods In total, 28 implants were placed in 28 patients requiring implantation in the posterior maxilla via a TSFE approach. A drill was used to access the planned position (within 1 mm of the bottom of the maxillary sinus floor) under dynamic navigation system guidance. TSFE was then accomplished using osteotomes and a piezoelectric device. Three effective deviations between planned and actual implant placement were then measured including angular deviation (AD, degrees), entry point horizontal deviation (EPHD, mm), and apical point horizontal deviation (APHD, mm).Results The AD, EPHD, and APHD between the planned and actual implant placement were 3.656±1.665 degrees, 1.073±0.686 mm, and 1.086±0.667 mm, respectively. Premolar site AD values were less than those for molar sites (P=0.004). No significant differences in these outcomes were observed when comparing procedures conducted by two different surgeons. Obvious sinus perforation was not detected by immediate postoperative cone-beam computed tomography imaging. Conclusions The accuracy associated with using a dynamic navigation system when conducting posterior maxilla implant surgery via a TSFE approach using piezoelectric devices was comparable to that observed for previously published conventional implant navigation approaches. This technique thus achieved appropriate interventional precision and safety while decreasing the morbidity associated with the TSFE approach.
m6A-related lncRNAs are potential biomarkers for predicting prognoses in patients with breast cancer
Authors: Liao Yuwei , Liang Simon X , He Dong , Chen Zhikai , Yao Jinxiu , Liang Hongfeng , Yang Liye 
Published: February 2022

Abstract
Background: Breast cancer (BRCA) is a common cancer and major cause of cancer mortality in females around the world. N6-methyladenosine (m6A) and m6A-associated long non-coding RNAs (lncRNAs) play vital roles in the prognostic value of BRCA. However, how m6A-related lncRNAs affect the survival of BRCA patients remains unclear. Methods: In this study, we obtained m6A-related lncRNAs by gene co-expression and used univariate least absolute shrinkage and selection operator (LASSO), and multivariate Cox regression analyses to construct a comprehensive m6A-related lncRNA model. Based on this model, a risk score was calculated for each patient. Next, Kaplan-Meier analyses, cell type enrichment analysis, and gene ontology (GO) enrichment were used to analyze the risk model. Finally, performance comparison was made between risk model and other common prognostic biomarkers in BRCA using ROC curve and nomogram. Results: The comprehensive risk model of 39 genes (10 m6A-related lncRNAs and 29 other genes) and basic clinical factors showed better performance in BRCA prognosis compared to other common prognostic biomarkers. By regrouping the patients with this model, we found that patients in the high-risk group had higher tumor mutation burden (TMB) and differentially expressed genes between high- and low risk patients were enriched in tumor-related inflammation response pathway and lipid droplet pathway. Conclusions: These results demonstrate that our comprehensive 39-genes risk model based on the m6A-related lncRNAs is promising for clinical prediction and provides a treatment guide for BRCA patients.
Differences in Gene Expression Between High- and Low-Grade Serous Ovarian Cancers: Implications for Diagnosis and Prognosis
Authors: Xu Jianhao , Wang Qian , Cao Fang , Deng Zhiyong , Gao Xiaojiao , Gu Tingting , Liu Tingting , Xu Song , Gan Wenjuan 
Published: February 2022

Abstract
Background: The clinical presentations of high-grade serous ovarian cancer (HGSOC) and low-grade serous ovarian cancer (LGSOC) differ. In this study, we aimed to identify the essential molecules for the diagnosis and prognosis of these OC subtypes. Methods: Differentially expressed genes between HGSOC and LGSOC were identified using three GEO series. The functional enrichment analysis was performed to investigate different biological processes and pathways. The protein-protein interaction network was constructed, and the discovered hub genes were frequently validated using prognostic correlation and immunohistochemistry (IHC) in GEPIA and HPA databases. Finally, we screened out BIRC5 and used IHC to detect its expression in 20 cases of borderline serous tumors, 20 cases of LGSOC, and 38 cases of HGSOC, and further analyzed its correlation with clinical characteristics.Results: In comparison with LGSOC, 79 upregulated and 85 downregulated genes were identified in HGSOC. The biological roles of these genes were mainly centered on the cell cycle process and chromosomal segregation. Among the 10 hub genes chosen, BIRC5 is positively related to the overall survival of patients with OC (p = 0.014) and can distinguish OC from normal ovarian tissue. In addition to database analysis, we verify BIRC5 through the specimen resources in our case database. According to the IHC results of our specimens, we found that BIRC5 can not only distinguish HGSOC and LGSOC but also positively correlate with the age, preoperative CA125 level, FIGO stage，and TP53/Ki-67 expression in tumor specimens.Conclusions: BIRC5 is a reliable marker that can distinguish HGSOC from LGSOC, guide prognosis, and be utilized in clinical IHC.
Prone Positioning May Increase Lung Overdistension in COVID-19-Induced ARDS
Authors: Otáhal Michal , Mlček Mikuláš , Borges João Batista , Alcala Glasiele , Hladík Dominik , Kuriščák Eduard , Tejkl Leoš , Amato Marcelo , Kittnar Otomar 
Published: February 2022

Abstract
Background: Real-time effects of changing body position and positive end-expiratory pressure (PEEP) on regional lung overdistension and collapse in individual patients remain largely unknown and not timely monitored. The aim of this study was to individualize PEEP in supine and prone body positions seeking to reduce lung collapse and overdistension in mechanically ventilated patients with coronavirus disease (COVID-19)-induced acute respiratory distress syndrome (ARDS). We hypothesized that prone positioning with bedside titrated PEEP would provide attenuation of both overdistension and collapse.Methods: In this prospective observational study, patients with COVID-19-induced ARDS under mechanical ventilation were included. We used electrical impedance tomography (EIT) with decremental PEEP titration algorithm (PEEPEIT-titration), which provides information on regional lung overdistension and collapse, along with global respiratory system compliance, to individualize PEEP and body position. PEEPEIT-titration in supine position straightaway followed by PEEPEIT-titration in prone position were performed. Immediately before each PEEPEIT-titration, the same lung recruitment maneuver was performed: 2 min of PEEP 24 cmH2O and driving pressure of 15 cmH2O.Results: Forty-two PEEPEIT-titration were performed in ten patients (21 pairs supine and prone positions). We have found larger % of overdistension along the PEEP titration in prone than supine position (P = 0.042). A larger % of collapse along the PEEP titration was found in supine than prone position (P = 0.037). A smaller respiratory system compliance was found in prone than supine position (P < 0.0005).Conclusions: In patients with COVID-19-induced ARDS, prone body position, when compared with supine body position, decreased lung collapse at low PEEP levels, but increased lung overdistension at PEEP levels greater than 10 cm H2O.Trial registration number: NCT04460859
Análisis sobre el uso de whatsapp en el entorno universitario: caso estudiantes universidad ecuatoriana
Authors: López Álvarez Diana María , Castro Aguilar Gilberto Fernando 
Published: February 2022

La investigación, utiliza la técnica de naturaleza cuantitativa y se llevó a cabo con la participación de 95 estudiantes de la carrera de Ingeniería en Sistemas de una universidad ecuatoriana matriculados en el año 2019. Como resultado se encontró que, sin importar sexo, desempeño académico, estatus social, carrera y por la facilidad de uso de la herramienta WhatsApp es muy utilizada por la mayor parte de los participantes de este estudio. A parte de su vida personal se puede constatar que la mayoría de los estudiantes usa este tipo de aplicaciones de mensajería instantánea también para cuestiones relacionadas con los asuntos académicos. Además, un alto porcentaje de los estudiantes analizados pertenece a algún chat grupal relacionado con los estudios o está incluido en grupos de conversación sobre asuntos académicos, investigación y extracurriculares.
principio de igualdad y no discriminación analizado desde la figura de la mujer como sujeto de derechos
Authors: Zuleta Sánchez Andrea Gabriela 
Published: February 2022

El presente artículo, tiene como objetivo general analizar a través de un estudio doctrinario y crítico, el principio de igualdad tanto formal como material, el principio de igualdad y no discriminación analizado desde la figura de la mujer como sujeto de derechos. El bloque de constitucionalidad, es decir, la Constitución junto con los instrumentos internacionales de derechos humanos ratificados por el Ecuador garantiza la igualdad como piedra angular del Estado constitucional de derechos. A través de la subsunción entre la norma y el hecho jurídico, se identifican políticas estatales dirigidas a la implementación real del principio de igualdad desde el enfoque de género, mediante un estudio doctrinario y normativo del derecho de los derechos humanos. Por lo que se concluye que el principio de igualdad no obstante se encuentra contenido en normativa tanto nacional como internacional debe ser ejercido por cada individuo, como titular de derechos, y que el Estado, como portador de obligaciones, es quien debe garantizar su aplicación efectiva en todos los ámbitos. La metodología empleada ha sido el uso del método deductivo y la investigación jurídica mixta (investigación teórica y empírica), utilizando además el derecho comparado.
مظاهر الدور التشريعي لمجلس الدولة
Authors: Hama Zana Rauf , Omar Rebin Abu Bakir 
Published: February 2022

تبحث هذه الدراسة في مظاهر دور مجلس الدولة في مجال التشريع بإعتباره أحدى الجهات المساهمة في سن التشريعات و صياغتها و الرقابة عليها، وقد أصبح موضوع مجلس الدولة في الدول التي تتبنى النظام القضائي المزدوج أكثر حضوراَ في مجال التشريع و بات تطوير الأعمال التشريعية والرقابية في الدولة أكثر أهمية وذلك في ضوء مساهمات مجلس الدولة لوضع الأطر القانونية لأعمال الإدارة العامة تطوير جودة التشريعات و الرقابة على أعمال الإدارة العامة لضمان الأمن القانوني و حماية مبدأ المشروعية.ومن هنا فإن المجلس يساهم من خلال دوره التشريعي في تطوير العمل التشريعي و تحسين الأداء المؤسسي للبرلمان والحكومة معاً، أي زيادة قدرات السلطتين التشريعية والتنفيذية لإصدار تشريعات أكثر نجاعة و أحسن جودة ، الأمر الذي يدعم عملية تفعيل دور هذه المؤسسات في الدولة.
Identification of Social Engagement Indicators Associated With Autism Spectrum Disorder Using a Game-Based Mobile App: Comparative Study of Gaze Fixation and Visual Scanning Methods
Authors: Varma Maya , Washington Peter , Chrisman Brianna , Kline Aaron , Leblanc Emilie , Paskov Kelley , Stockham Nate , Jung Jae-Yoon , Sun Min Woo , Wall Dennis P 
Published: February 2022

Background
Autism spectrum disorder (ASD) is a widespread neurodevelopmental condition with a range of potential causes and symptoms. Standard diagnostic mechanisms for ASD, which involve lengthy parent questionnaires and clinical observation, often result in long waiting times for results. Recent advances in computer vision and mobile technology hold potential for speeding up the diagnostic process by enabling computational analysis of behavioral and social impairments from home videos. Such techniques can improve objectivity and contribute quantitatively to the diagnostic process.

Objective
In this work, we evaluate whether home videos collected from a game-based mobile app can be used to provide diagnostic insights into ASD. To the best of our knowledge, this is the first study attempting to identify potential social indicators of ASD from mobile phone videos without the use of eye-tracking hardware, manual annotations, and structured scenarios or clinical environments.

Methods
Here, we used a mobile health app to collect over 11 hours of video footage depicting 95 children engaged in gameplay in a natural home environment. We used automated data set annotations to analyze two social indicators that have previously been shown to differ between children with ASD and their neurotypical (NT) peers: (1) gaze fixation patterns, which represent regions of an individual’s visual focus and (2) visual scanning methods, which refer to the ways in which individuals scan their surrounding environment. We compared the gaze fixation and visual scanning methods used by children during a 90-second gameplay video to identify statistically significant differences between the 2 cohorts; we then trained a long short-term memory (LSTM) neural network to determine if gaze indicators could be predictive of ASD.

Results
Our results show that gaze fixation patterns differ between the 2 cohorts; specifically, we could identify 1 statistically significant region of fixation (P<.001). In addition, we also demonstrate that there are unique visual scanning patterns that exist for individuals with ASD when compared to NT children (P<.001). A deep learning model trained on coarse gaze fixation annotations demonstrates mild predictive power in identifying ASD.

Conclusions
Ultimately, our study demonstrates that heterogeneous video data sets collected from mobile devices hold potential for quantifying visual patterns and providing insights into ASD. We show the importance of automated labeling techniques in generating large-scale data sets while simultaneously preserving the privacy of participants, and we demonstrate that specific social engagement indicators associated with ASD can be identified and characterized using such data.