bluuebunny/crossref_metadata_2025_split huggingface dataset:
The HSP90 inhibitor KW-2478 depletes the malignancy of BCR/ABL and overcomes the imatinib-resistance caused by BCR/ABL amplification
Authors: Zeng Dachuan , Gao Miao , Zheng Renren , Qin Run , He Wei , Liu Suotian , Wei Wei , Huang Zhenglan 
Published: May 2022

Abstract
Background
With the widespread clinical application of tyrosine kinase inhibitors (TKIs), an increasing number of chronic myeloid leukaemia (CML) patients have developed resistance or intolerance to TKIs. BCR/ABL is the oncoprotein of CML. HSP90 is an essential chaperone of BCR/ABL and plays an important role in protein folding and the function of BCR/ABL. Therefore, inhibiting the chaperone function of HSP90 may be an effective strategy for CML treatment and to overcome TKI resistance.

Methods
The effect of KW-2478 on CML cell viability, apoptosis and cell cycle progression was detected by CCK-8 assay or flow cytometry. The levels of BCR/ABL, HSP90 and other signalling proteins were detected by western blots. The mitochondrial membrane potential was detected by flow cytometry combined with JC-1 staining. The interaction between BCR/ABL and HSP90α was detected by coimmunoprecipitation. The effect of KW-2478 on BCR/ABL carcinogenesis in vivo was investigated in CML-like mouse models.

Results
KW-2478 inhibited growth and induced apoptosis of CML cells. KW-2478 inhibited the chaperone function of HSP90α and then weakened the BCR/ABL and MAPK signalling pathways. This treatment also caused an increase in p27 and p21 expression and a decrease in cyclin B1 expression, which led to G2/M phase arrest. The mitochondrial pathway was primarily responsible for KW-2478-induced apoptosis. KW-2478 had a synergistic effect with imatinib in growth inhibition. Notably, KW-2478 had a stronger effect on growth inhibition, apoptosis induction and cell cycle arrest of K562/G01 cells than K562 cells. KW-2478 could effectively prolong the mouse lifespan and alleviate disease symptoms in CML-like mouse models.

Conclusions
This finding demonstrated that KW-2478 had anticancer properties in imatinib-sensitive and imatinib-resistant CML cells and illustrated the possible mechanisms. This study provides an alternative choice for CML treatment, especially for TKI-resistant patients with BCR/ABL amplification and TKI-intolerant patients.
Redevelopment of mental health first aid guidelines for supporting someone experiencing a panic attack: a Delphi study
Authors: Chalmers Kathryn J. , Rossetto Alyssia , Reavley Nicola J. , Jorm Anthony F. , Kitchener Betty A. , Kelly Claire M. , Morgan Amy J. , Bond Kathy S. , Cottrill Fairlie A. 
Published: May 2022

Abstract
Background
Panic attacks and panic disorder can have a major impact on the mental health and wellbeing of those who experience them. People with recurrent panic attacks have increased odds of developing a mental disorder and of worsening the course of existing mental disorders. Early intervention efforts at the time that a panic attack occurs might reduce or prevent some of these associated negative outcomes. Expert consensus guidelines for high income Western countries on how to provide mental health first aid for panic attacks were published in 2009. The present study aims to redevelop these guidelines to ensure content reflects current evidence and best practice.

Methods
The Delphi consensus method was used to determine which helping strategies should be included in the redeveloped guidelines. A survey with items on how to assist someone who is having a panic attack was developed using the 2009 guidelines and a systematic search of grey and academic literature. Panellists with lived experience and professional experience rated these items to determine which helping statements should be included in the guidelines.

Results
Thirty panellists completed all three surveys. Panellists rated 158 statements, with 83 statements meeting the criteria for inclusion in the redeveloped guidelines. The endorsed statements covered: what the first aider should know about panic attacks, what they should do if they think someone is having a panic attack, what they should do if they are uncertain whether the person is having a panic attack, what they should say and do if they know the person is having a panic attack and what they should do when the panic attack has ended.

Conclusion
This study has resulted in a more comprehensive set of guidelines than the original version, with the endorsement of 83 helping actions, compared to 27 previously. The redeveloped guidelines provide greater detail on recognising the signs of a panic attack, providing initial assistance, communicating with someone experiencing a panic attack and supporting them to seek appropriate professional help if it is needed. ﻿The guidelines will be used in future updates of Mental Health First Aid training courses.
“Be perfect in every respect”: the mediating role of career adaptability in the relationship between perfectionism and career decision-making difficulties of college students
Authors: Chen Huaruo , Pang Liman , Liu Fei , Fang Tingting , Wen Ya 
Published: May 2022

Abstract
Background
Considering the weakening of the economy and the shrinkage of jobs under the current global epidemic crisis, the employment of college graduates is facing unprecedented and cruel competition. However, many college students lack a reasonable understanding and orientation of themselves, which leads to them having high expectations for future careers and not considering whether they are competent or not. Due to a lack of ability and self-confidence, they appear to be at a loss and hesitant when facing career choices. Therefore, the purpose of this study is to explore the relationship between perfectionism, career adaptability and career decision-making difficulties from both positive and negative aspects.

Methods
We sampled 400 college students in China and used a questionnaire to obtain cross-sectional data through the Perfectionism, Career Adaptability, and Career Decision Difficulties scales. This study explored the relationship between them using statistical analysis software such as SPSS and AMOS through the results of correlation analysis and mediating role analysis.

Results
College students’ perfectionism, career adaptability, and career decision-making difficulties were significantly correlated (p < 0.01). Positive perfectionism has a negative predictive effect on career decision-making difficulties, and career adaptability plays a completely mediating role in it (the direct effect is −0.032, the mediation effect is −0.244, and the total effect is −0.276.). Negative perfectionism plays a positive predictive role in career decision-making difficulties, and career adaptability plays a part in mediating roles (the direct effect is 0.334, the mediating effect is 0.099, and the total effect is 0.433.).

Conclusions
College students will more or less pursue “perfect”, but often with their own preferences to pursue, cannot be done based on the rational analysis of things to pursue perfect. College students have higher positive perfectionism and negative perfectionism, and their career adaptability is also at a higher level, but there is a higher degree of career decision-making difficulties. Positive perfectionism of college students can reduce the difficulty of career decision-making, and career adaptability plays a completely mediating role in it. Negative perfectionism of college students will lead to difficulties in career decision-making, in which career adaptability plays a mediating role.
Activated Drp1 regulates p62-mediated autophagic flux and aggravates inflammation in cerebral ischemia-reperfusion via the ROS-RIP1/RIP3-exosome axis
Authors: Zeng Xue , Zhang Yun-Dong , Ma Rui-Yan , Chen Yuan-Jing , Xiang Xin-Ming , Hou Dong-Yao , Li Xue-Han , Huang He , Li Tao , Duan Chen-Yang 
Published: May 2022

Abstract
Background
Cerebral ischemia-reperfusion injury (CIRI) refers to a secondary brain injury that can occur when the blood supply to the ischemic brain tissue is restored. However, the mechanism underlying such injury remains elusive.

Methods
The 150 male C57 mice underwent middle cerebral artery occlusion (MCAO) for 1 h and reperfusion for 24 h, Among them, 50 MCAO mice were further treated with Mitochondrial division inhibitor 1 (Mdivi-1) and 50 MCAO mice were further treated with N-acetylcysteine (NAC). SH-SY5Y cells were cultured in a low-glucose culture medium for 4 h under hypoxic conditions and then transferred to normal conditions for 12 h. Then, cerebral blood flow, mitochondrial structure, mitochondrial DNA (mtDNA) copy number, intracellular and mitochondrial reactive oxygen species (ROS), autophagic flux, aggresome and exosome expression profiles, cardiac tissue structure, mitochondrial length and cristae density, mtDNA and ROS content, as well as the expression of Drp1-Ser616/Drp1, RIP1/RIP3, LC3 II/LC3 I, TNF-α, IL-1β, etc., were detected under normal or Drp1 interference conditions.

Results
The mtDNA content, ROS levels, and Drp1-Ser616/Drp1 were elevated by 2.2, 1.7 and 2.7 times after CIRI (P < 0.05). However, the high cytoplasmic LC3 II/I ratio and increased aggregation of p62 could be reversed by 44% and 88% by Drp1 short hairpin RNA (shRNA) (P < 0.05). The low fluorescence intensity of autophagic flux and the increased phosphorylation of RIP3 induced by CIRI could be attenuated by ROS scavenger, NAC (P < 0.05). RIP1/RIP3 inhibitor Necrostatin-1 (Nec-1) restored 75% to a low LC3 II/LC3 I ratio and enhanced 2 times to a high RFP-LC3 after Drp1 activation (P < 0.05). In addition, although CIRI-induced ROS production caused no considerable accumulation of autophagosomes (P > 0.05), it increased the packaging and extracellular secretion of exosomes containing p62 by 4 – 5 times, which could be decreased by Mdivi-1, Drp1 shRNA, and Nec-1 (P < 0.05). Furthermore, TNF-α and IL-1β increased in CIRI-derived exosomes could increase RIP3 phosphorylation in normal or oxygen–glucose deprivation/reoxygenation (OGD/R) conditions (P < 0.05).

Conclusions
CIRI activated Drp1 and accelerated the p62-mediated formation of autophagosomes while inhibiting the transition of autophagosomes to autolysosomes via the RIP1/RIP3 pathway activation. Undegraded autophagosomes were secreted extracellularly in the form of exosomes, leading to inflammatory cascades that further damaged mitochondria, resulting in excessive ROS generation and the blockage of autophagosome degradation, triggering a vicious cycle.
Coexisting good neighbours: acoustic and calling microhabitat niche partitioning in two elusive syntopic species of balloon frogs, Uperodon systoma and U. globulosus (Anura: Microhylidae) and potential of individual vocal signatures
Authors: Prasad Vishal Kumar , Chuang Ming-Feng , Das Abhijit , Ramesh K. , Yi Yoonjung , Dinesh K. P. , Borzée Amaël 
Published: May 2022

Abstract
Background
Most amphibians use a repertoire of acoustic signals to propagate signals in social contexts. The description of these repertoires provides a key towards the understanding of the behaviour of individuals and the evolutionary functions of calls. Here, we assessed the variations in advertisement calls within and between two fossorial sympatric species, Uperodon systoma and Uperodon globulosus, that share their breeding season and breeding sites. For each species, we applied Beecher’s index of total information capacity (HS) for the individual vocal signature, determined the difference in call properties and demonstrated the segregation in the calling microhabitat niche between the two species.

Results
Our results demonstrated that the advertisement calls of U. systoma are pulsatile with a call rate of 3.00 ± 0.97 calls per second while those of U. globulosus are not pulsatile with a lower call rate of 0.53 ± 0.22 calls per second. For both species, the variations in call properties among individuals was higher than that within individual, a pattern consistent with that of other fossorial anurans. The body condition and air temperature did not significantly impact the call properties of either species. The outcome of the Beecher’s index (HS) showed that the calls of U. systoma can be used to identify 14 different individuals and the calls of U. globulosus can be used to identify 26 different individuals. The statistical analyses on the advertisement call of the two species showed a significant difference in the temporal properties as the call duration, and fall time and rise time were significantly different between the two species. Lastly, we successfully demonstrated that there is a clear segregation in calling site microhabitat between the two species, where U. globulosus calls floating close to the bank of the waterbody while U. systoma calls floating further away from the bank.

Conclusion
This study highlights the potential for pre-mating isolation, character displacement and assortative mating in two syntopic fossorial anurans, leading to association between acoustic, calling microhabitat niche and body index divergence as important behavioural and ecological traits.
Biological control potential of local entomopathogenic nematodes against the different stage larvae of cotton leafworm Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae)
Authors: Yağcı Mürşide , Yücel Cenk , Erdoğuş F. Dolunay , Benk Gökhan , Kepenekci İlker 
Published: May 2022

AbstractBackgroundThe cotton leafworm,Spodoptera littoralis(Boisduval) (Lepidoptera: Noctuidae), is an economic pest on various crops worldwide. Farmers generally used to apply chemical pesticides to control the pest. The bio-control potential of the entomopathogenic nematodes (EPNs) as alternatives to harmful synthetic pesticides was examined in this study. The study aimed to determine the efficacy of EPNs isolates against the different stages of larvae of the cotton leafwormS. littoralisand the effect of time on mortality rate under laboratory conditions.ResultsEPNs isolates were tested at 4 different concentrations (0, 250, 500 and 1000 IJs/ml) in 150 ml plastic containers on last instar larvae (sixth instar) ofS. litorallis. Experiments for third, fourth and fifth instar larvae ofS. litoralliswere carried out in petri dishes at different concentrations (0, 50, 100 and 200 IJs/ml) at 25 °C temperature. Mortality rates of larvae were calculated at 3 different times (48, 72, 96 h) after inoculation. The high mortality rate of last instar larvae (98.81%) ofS. littoraliswas caused bySteinernema feltiae(Tokat-Emir), followed byS. carpocapsae(Tokat-Bakışlı05) (95.24%) and thenH. bacteriophora(11 KG) with (90.47%) at the highest concentration (1000 IJ/ml). The highest mortality rate of fifth instar larvae was caused byS. feltiae(Tokat-Bakışlı05) andS. carpocapsae(Tokat-Emir) with (100%) and (92.12%). In addition, the highest mortality rate of the 4th instar larvae was determined S.feltiae(Tokat-Bakışlı05) and S.carpocapsae(Tokat-Emir) isolates (98.87%) and (97.74%), respectively. Additionally, the highest larval mortality rate in the third stage byS. feltiae(Tokat-Bakışlı05) andS. carpocapsae(Tokat-Emir) was (100%) and (97.74%) at the highest concentration. Mortality rates of larvae were calculated at 3 different times after inoculation. The highest mortality rate counted in all isolates was determined 96 and 72 h after inoculation of EPNs.ConclusionsAll indigenous EPN isolates were found to be effective at different rates againstS. littoralis. The results showed that these nematode species could be used againstS. littoralisbiological control programs.
Percutaneous debulking strategy for severe nodular calcification in common femoral artery
Authors: Nomura Tetsuya , Ota Issei , Tasaka Satoshi , Ono Kenshi , Sakaue Yu , Shoji Keisuke , Wada Naotoshi 
Published: May 2022

Abstract
Background
Despite marked progress in endovascular treatment (EVT) techniques and devices, calcified lesions remain one of the toughest obstacles to EVT success. Moreover, because the common femoral artery (CFA) is known as a “non-stenting zone,” endovascular strategies for this area are controversial.

Case presentation
Here we describe the technical tips for a novel, less invasive, and effective debulking strategy for severe nodular calcification using an endovascular maneuver. This technique was demonstrated in a 73-year-old man with severe calcified stenosis of the CFA. To complete a stent-less strategy for CFA, we conducted aggressive debulking of the nodular calcification, established a bidirectional approach from the radial artery and the superficial femoral artery (SFA), and inserted a balloon-guiding catheter in the SFA. Under distal protection provided by this catheter, we crushed the nodular calcification 43 times using myocardial biopsy forceps. After achieving a volume reduction of nodular calcification through this maneuver, we completed the procedure by inflating a 6-mm drug-coated balloon catheter. Final angiography demonstrated a reduced filling defect of the contrast medium in the CFA and favorable blood flow as far as the ankle. The puncture site on the SFA was closed with a vascular suture assisted by balloon inflation inside the vessel, which allowed the patient to be ambulatory immediately after the procedure without requiring bed rest.

Conclusions
Severely calcified lesions in the CFA are usually difficult to treat using an endovascular strategy, but our novel and less invasive method may become a promising technique for managing these lesions.
The assessment and management of pica in people with intellectual disability
Authors: Williams Fionnuala , Gibbs Susie , Addo Ama S. 
Published: May 2022

SUMMARYPica is a condition associated with a number of physical and mental health diagnoses. The potentially fatal consequences of pica and the links with significant physical health problems are not always recognised. Pica is like other forms of behaviour that can challenge: clinicians must seek the underlying cause and treat this first, before primarily pursuing a behavioural form of treatment. In this article, we discuss the associations, consequences, assessment and management options available for pica to guide professionals. Pica often presents in a way individual to the particular patient, so tailoring of assessment and treatment is important.
From head to rootlet: comparative transcriptomic analysis of a rhizocephalan barnacle Peltogaster reticulata (Crustacea: Rhizocephala)
Authors: Nesterenko Maksim , Miroliubov Aleksei 
Published: May 2022

Background: Rhizocephalan barnacles stand out in the diverse world of metazoan parasites. The body of a rhizocephalan female is modified beyond revealing any recognizable morphological features, consisting of the interna, the system of rootlets, and the externa, a sac-like reproductive body. Moreover, rhizocephalans have an outstanding ability to control their hosts, literally turning them into “zombies”. Despite all these amazing traits, there is no genomic and transcriptomic data about any Rhizocephala. Methods: We collected transcriptomes from four body parts of an adult female rhizocephalan Peltogaster reticulata: externa and main, growing, and thoracic parts of the interna. We used all prepared data for the de novo assembly of the reference transcriptome. Next, a set of encoded proteins was determined, the expression levels of protein-coding genes in different parts of the parasite body were calculated and lists of enriched bioprocesses were identified. We also in silico identified and analyzed sets of potential excretory / secretory proteins. Finally, we applied phylostratigraphy and evolutionary transcriptomics approaches to our data. Results: The assembled reference transcriptome included transcripts of 12,620 protein-coding genes and was the first for both P. reticulata and Rhizocephala. Based on the results obtained, the spatial heterogeneity of protein-coding genes expression in different regions of P. reticulata adult female body was established. The results of both transcriptomic analysis and histological studies indicated the presence of germ-like cells in the lumen of the interna. The potential molecular basis of the interaction between the nervous system of the host and the parasite's interna was also determined. Given the prolonged expression of development-associated genes, we suggest that rhizocephalans “got stuck in the metamorphosis”, even in their reproductive stage. Conclusions: The results of the first comparative transcriptomic analysis for Rhizocephala not only clarified but also expanded the existing ideas about the biology of this amazing parasites.
Color translation from monoscopic photogrammetry +ID Methodology into a Polyjet final 3D printed facial prosthesis.
Authors: Salazar-Gamarra Rodrigo , Cárdenas-Bocanegra Andrés , Masch Uri , Da Costa Moraes Cícero André , Seelaus Rosemary , Lopes Da Silva Jorge Vicente , Lauria Dib Luciano 
Published: May 2022

Background: The artistic techniques necessary to fabricate facial prostheses mainly depend on individual skill and are not a resource easily reproduced. Digital technology has contributed to improved outcomes, often combining analog and new digital techniques in the same workflow. Methods: This article aims to present an innovative workflow to produce a final colored 3D printed and facial prosthesis by UV-map color translation into colored resin 3D printing. A modified +ID Methodology was used to obtain 3D models with the calibrated 3D printable patient’s skin color. No hands-on physical molding, manual sculpture, or intrinsic silicone coloration was used. Results: The outcome resulted in acceptable aesthetics, adaptation, and an approximate color match after extrinsic coloration. The patient reported good comfort and acceptance. Conclusions: A direct resin 3D printed prosthesis may be a viable alternative, especially for rapid delivery as an immediate prosthesis or an option when there is no experienced anaplastogist to manufacture a conventional prosthesis.
COVID – 19 and psychiatry teaching during the outbreak of the pandemic at the Eduardo Mondlane Medical School
Authors: Pedro Maria Salomão , Palha Antonio , Ferreira Maria 
Published: May 2022

Background: The COVID-19 pandemic had a considerable impact on the lives of the world's population, which led to the closure of educational institutions including in Mozambique. In March 2020, the state emergency forced students and professors to change in-person classes to online learning because of the pandemic. Objectives: To access students’ capacities in terms of technological resources to participate in the 5th-year online classes of the Eduardo Mondlane University Medical School and the difficulties they encountered throughout the year. Additionally, psychological symptoms associated with confinement and how that affected participation in psychiatry and mental health classes were assessed. Methods: A cross sectional social online questionnaire survey was conducted among 32 students enrolled in the 5th-year psychiatry and mental health classes of the Eduardo Mondlane University Medical School during May and June 2021. Results: A total of 47 students were invited to participate in this survey, of which 32 students (68%) participated. Of the participants, 16.7% reported the presence of psychological symptoms associated with confinement. All students could participate in online classes using cell phones, computers, and tablets. However, 34.4% did not have a laptop. In this study, 87.5% of the respondents reported poor internet quality, and 12.5% ​​of students did not have internet and had to join colleagues to participate in classes and to interact with the members of their groups. Most of the students (90.6%) were not prepared or knew how to use google classroom, Skype, and Zoom, before the lockdowns. Conclusions: The study suggests that the abrupt and radical change from in-person teaching and learning methods to remote online methods showed the weaknesses of students in terms of resources (computers, tablets, internet) and knowledge for the implementation of online classes. Psychological symptoms were present but did not affect student participation in remote psychiatry and mental health classes.