bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunomodulatory fibrous hyaluronic acid‐Fmoc‐diphenylalanine‐based hydrogel induces bone regeneration
Authors: Halperin‐Sternfeld Michal , Pokhojaev Ariel , Ghosh Moumita , Rachmiel Dana , Kannan Raha , Grinberg Itzhak , Asher Moshe , Aviv Moran , Ma Peter X. , Binderman Itzhak , Sarig Rachel , Adler‐Abramovich Lihi 
Published: September 2022

AbstractAimTo investigate the potential of an ultrashort aromatic peptide hydrogelator integrated with hyaluronic acid (HA) to serve as a scaffold for bone regeneration.Materials and MethodsFluorenylmethyloxycarbonyl‐diphenylalanine (FmocFF)/HA hydrogel was prepared and characterized using microscopy and rheology. Osteogenic differentiation of MC3T3‐E1 preosteoblasts was investigated using Alizarin red, alkaline phosphatase and calcium deposition assays. In vivo, 5‐mm‐diameter calvarial critical‐sized defects were prepared in 20 Sprague–Dawley rats and filled with either FmocFF/HA hydrogel, deproteinized bovine bone mineral, FmocFF/Alginate hydrogel or left unfilled. Eight weeks after implantation, histology and micro‐computed tomography analyses were performed. Immunohistochemistry was performed in six rats to assess the hydrogel's immunomodulatory effect.ResultsA nanofibrous FmocFF/HA hydrogel with a high storage modulus of 46 KPa was prepared. It supported osteogenic differentiation of MC3T3‐E1 preosteoblasts and facilitated calcium deposition. In vivo, the hydrogel implantation resulted in approximately 93% bone restoration. It induced bone deposition not only around the margins, but also generated bony islets along the defect. Elongated M2 macrophages lining at the periosteum–hydrogel interface were observed 1 week after implantation. After 3 weeks, these macrophages were dispersed through the regenerating tissue surrounding the newly formed bone.ConclusionsFmocFF/HA hydrogel can serve as a cell‐free, biomimetic, immunomodulatory scaffold for bone regeneration.
Medical Device‐Related Pressure injuries in adult intensive care units
Authors: Celik Selda , Taskin Yilmaz Feride , Altas Gulnaz 
Published: September 2022

AbstractAim and ObjectiveThis study was conducted to examine the development, characteristics, and risk factors of medical device‐related pressure injury (MDRPI) in intensive care units (ICU).BackgroundThe number of individuals admitted to ICU increased in the last years all over the world. In parallel with this need, the frequency of the use of life‐support and therapeutic medical devices in the ICU also increases. This situation may lead to the development of MDRPI in the ICU and an increase in its prevalence.MethodThe study, which was conducted observationally, prospectively and descriptively, included 302 patients who were hospitalised in an ICU within a year. The study was reported according to the STROBE Declaration.ResultsIt was observed that MDRPI developed in 27.2% of the patients. It was found that MDRPI developed the most in the nose (26.8%) and mouth (15.9%) regions of the patients. It was determined that MDRPI was diagnosed in 28% of the patients within 3–5 days. It was determined that mostly orthopaedic devices (plaster, cervical collar, splint) (62.5%), fasteners (57.1%), non‐invasive ventilation/oxygen masks (51.2%) caused the development of MDRPI. It was found that the number of medical devices used was higher, and the number of hospitalisation days in the ICU was also higher, and these factors explained 28.3% of the total variance in the development of MDRPI.ConclusionIt was determined that MDRPI developed in approximately one of four patients in the ICU and that the numbers of medical devices and hospitalisation days were important determinant risk factors.Relevance to Clinical PracticeThe high rate of development of MDRPI is worrying in terms of nursing care quality. It is recommended that nurses recognise risk factors in order to prevent the development of MDRPI, evaluate the suitability, necessity, and safety of the devices to be used is located.
Effect of induced saliva flow on fluid retention time, ruminal microbial yield and methane emission in cattle
Authors: Zhang Xiaoyu , Li Yang , Terranova Melissa , Ortmann Sylvia , Kehraus Saskia , Gerspach Christian , Kreuzer Michael , Hummel Jürgen , Clauss Marcus 
Published: September 2022

AbstractBoth in vitro and animal studies indicated that a higher dilution rate is related to a more efficient microbial synthesis and a lower methane (CH4) yield. The latter could be a consequence of the former, as an increase in microbial cell synthesis offers an alternative hydrogen sink competing with methanogenesis. To test this assumption in live animals, we applied a saliva stimulant, pilocarpine, to modify liquid flow rate in cattle. Four non‐lactating cows (750 ± 71 kg) were fed forage only (restricted to constant intake) in a 4 × 4 Latin square design with oral doses of 0, 1, 2.5 and 5mg pilocarpine/kg body weight and day. We quantified feed and water intake, ruminal and total tract mean retention time (MRT) of solute and particle markers, ruminal microbial yield (via urinary purine bases or metabolic faecal nitrogen), CH4 emission, digestibility, chewing behaviour, reticular motility and rumen fluid parameters. The effect of induced saliva flow was evident by visibly increased salivation and water intake. Increasing the pilocarpine dosages resulted in a linearly decreased MRT of fluid and small particles (p < 0.001 and p< 0.05, respectively) and methane yield as related to digested DM (p < 0.05), the latter at a magnitude of 5%. No effect of treatment was found on ruminal microbial yield estimated via purine derivates. Metabolic faecal N as an indicator of microbial growth linearly correlated with pilocarpine dosages (p < 0.05). No significant relationship was found between pilocarpine dosages and large particle MRT, nutrient digestibility, ruminal pH and short‐chain fatty acids. In conclusion, different from some in vitro studies, there was little indication of a reciprocal effect of CH4 and microbial biomass production in cows fed a forage‐only diet.
Understanding Words to Change Behaviour
Authors: Elizabeth Margaret 
Published: September 2022

AbstractWhy do people in churches exclude, alienate, and even abuse others? Acknowledging the need for many ways of addressing this question, insights from the study of language have been explored, creating a synthesis from the ways in which ideology and language are related, an adaptation of Judith Butler's exploration of our constitution in language, understandings within the discipline of psycholinguistics, and insights from Wittgenstein's philosophy of language. This synthesis shows how words to and for the divine influence our behaviour to each other and indicates that the traditional words of male hierarchy uphold an ideology that seeks to maintain power structures, with behaviours that exclude, alienate, and even abuse fitting within these structures. The synthesis demonstrates both the need for and possibility of change from the traditionally used words to and for the divine.
Two Arabidopsis MYB‐SHAQKYF transcription repressors regulate leaf wax biosynthesis via transcriptional suppression on DEWAX
Authors: Liu Qing , Huang Haodong , Chen Yongqiang , Yue Zhichuang , Wang Zhipeng , Qu Tingting , Xu Danyun , Lü Shiyou , Hu Honghong 
Published: September 2022

Summary

Plant cuticular wax accumulation limits nonstomatal transpiration and is regulated by external environmental stresses. DEWAX (DECREASE WAX BIOSYNTHESIS) plays a vital role in diurnal wax biosynthesis. However, how DEWAX expression is controlled and the molecular mechanism of wax biosynthesis regulated by the diurnal cycle remains largely unknown. Here, we identified two Arabidopsis MYB‐SHAQKYF transcription factors, MYS1 and MYS2, as new regulators in wax biosynthesis and drought tolerance.

Mutations of both MYS1 and MYS2 caused significantly reduced leaf wax, whereas overexpression of MYS1 or MYS2 increased leaf wax biosynthesis and enhanced drought tolerance. Our results demonstrated that MYS1 and MYS2 act as transcription repressors and directly suppress DEWAX expression via ethylene response factor‐associated amphiphilic repression motifs.

Genetic interaction analysis with DEWAX, SPL9 (SQUAMOSA PROMOTER BINDING PROTEIN‐LIKE 9), and CER1 (ECERIFERUM 1) in wax biosynthesis and under drought stresses demonstrated that MYS1 and MYS2 act upstream of the DEWAX‐SPL9 module, thus regulating CER1 expression. Expression analysis suggested that the diurnal expression pattern of DEWAX is partly regulated by MYS1 and MYS2.

Our findings demonstrate the roles of two unidentified transcription repressors, MYS1 and MYS2, in wax biosynthesis and provide insights into the mechanism of diurnal cycle‐regulated wax biosynthesis.
Nitrogen‐inducibleGLK1modulates phosphate starvation response via thePHR1‐dependent pathway
Authors: Li Yan , Li Yanling , Yao Xiuhong , Wen Yu , Zhou Zuxu , Lei Wei , Zhang Dawei , Lin Honghui 
Published: September 2022

SummaryPhosphorus (P) is a limiting nutrient for plant growth and productivity. Thus, a deep understanding of the molecular mechanisms of plants' response to phosphate starvation is significant when breeding crops with higher phosphorus‐use efficiency.Here, we found that GARP‐type transcription factor GLK1 acted as a positive regulator for phosphate‐starvation response (PSR) via the PHR1‐dependent pathway inArabidopsis thaliana. GLK1 increased the transcription activity of PHR1 through the direct physical interaction and regulated the multiple responses to inorganic orthophosphate (Pi) starvation.Nitrogen (N) is a key factor in the regulation of PSR. We also found that the N status controlled the function of the GLK1‐PHR1 signaling module under Pi‐deficient (LP) conditions by regulating the accumulation of GLK1 and PHR1. Ultimately, we showed that the presence of GLK1 effectively promoted the protein accumulation of PHR1 at low N concentrations, and this action was helpful to maintain the activation of PSR.According to these findings, we establish the working model for GLK1 in PSR and propose that GLK1 mediates the interaction between N and P by influencing the effect of N on PHR1 inArabidopsis thaliana.
Repeat ablation strategy for recurrent persistent atrial fibrillation: A propensity‐matched score comparison between “2C3L” and “extensive ablation” approach
Authors: Lin Jing , Jiang Chen‐Xi , Long De‐Yong , He Liu , Sang Cai‐Hua , Yu Rong‐Hui , Tang Ri‐Bo , Li Song‐Nan , Guo Xue‐Yuan , Wang Wei , Liu Nian , Du Xin , Dong Jian‐Zeng , Ma Chang‐Sheng 
Published: September 2022

AbstractBackgroundDebates exist in the repeat ablation strategy for patients with recurrence presenting as persistent atrial fibrillation (AF) after initial persistent AF ablation.ObjectiveTo compare the outcome between the “2C3L” and “extensive ablation” approach in patients undergoing repeat procedures for recurrent persistent AF.MethodsPropensity‐score matching was performed in 196 patients with AF recurrence undergoing repeat ablation, and 79 patients treated with “2C3L” strategy were matched to 79 patients treated with “extensive ablation” strategy. The “2C3L” approach included pulmonary vein isolation, mitral isthmus, left atrial roof, and cavotricuspid isthmus ablation, while the “extensive ablation” strategy included extensive ablation of a variety of other targets aiming to terminate the AF. The primary outcome was freedom from any atrial tachyarrhythmia after 24‐h ambulatory monitoring follow‐up for 12 months.ResultsNo statistically significant difference was found between the primary outcome between the “2C3L” and the “extensive ablation” group [70.9% vs. 69.6%, p = .862; 95% confidence interval (CI) ‐12.8 to 15.3], although the “extensive ablation” group had a significantly high proportion of AF termination (19.0% for “2C3L” vs. 41.8% for “extensive ablation” group, p = .002; 95% CI 8.5–35.9). And AF termination was not related to the primary outcome in multifactorial regression. At 40 ± 22 months after the repeat procedure, the primary outcome was also comparable (57.0 % for “2C3L” vs. 48.1% for “extensive ablation” group, p = .265; 95% CI ‐6.6 to 23.7).ConclusionThe outcome between the “2C3L” and “extensive ablation” approaches was comparable in patients undergoing repeat procedures for recurrent persistent AF.
Transcriptome dynamics of ricein natura: Response of above and below ground organs to microclimate
Authors: Matsunami Maya , Murai‐Hatano Mari , Kuwagata Tsuneo , Matsushima Uzuki , Hashida Yoichi , Tominaga Yoko , Masuya Yusuke , Nagano Atsushi J. 
Published: September 2022

AbstractThe long‐term dynamics of the transcriptome under natural field conditions remain unclear. We conducted comprehensive gene expression analyses of rice leaves and roots grown under natural field conditions for a long period, from the tillering stage to the ripening stage. In this experiment, changes in the transcriptome were captured in relation to microclimatic parameters, particularly potential evaporation (Ep), which is a multiple meteorological factor and acts as an indicator of transpirational demand. The results indicated that many genes were regulated by changes in temperature and Ep in both leaves and roots. Furthermore, the correlation between gene expression and meteorological factors differed significantly between the vegetative and reproductive stages. Since Ep triggers transpiration, we analyzed aquaporin gene expression, which is responsible for water transport, and found that many aquaporin genes in leaves were positively correlated with Ep throughout the growth period, whereas in roots, two plasma membrane intrinsic aquaporins,PIP2;4andPIP2;5were strongly correlated with Ep during reproductive growth. Other genes closely related to productivity, such as those involved in nutrient absorption and photosynthesis, exhibited different responses to meteorological factors at different growth stages. The stage‐dependent shift in the microclimate response provides an important perspective on crop physiology in light of climate change.
A critical discourse analysis of the influence of organisational structures on inequality in head and neck cancer treatment in Denmark
Authors: Mondahl Julie , Thomsen Thora Grothe , Hellesø Ragnhild , Frederiksen Kirsten 
Published: September 2022

AbstractBackgroundConcern is growing about inequality in cancer treatment, and a call has been made for more knowledge of the underlying causes of this inequality. Studies show that patients with low socioeconomic status in general face a greater risk of inequality than patients with a high socioeconomic status.AimThe aim of the present study was to uncover how institutional factors may exacerbate inequality in cancer treatment for patients with low socioeconomic status exemplified by patients with head and neck cancer, most of whom have low socioeconomic status.MethodInspired by Fairclough, we undertook a critical discourse analysis investigating the treatment pathway of patients with head and neck cancer on the basis of policy papers.ResultsThese papers, which we conceived as formative instruments, harboured a discourse of efficiency and a discourse of participation, together carving out an effective cancer treatment pathway provided patients act in line with the recommendations.DiscussionThe discourses of efficiency and participation are not unfamiliar in health care, and prior research shows that they may pose difficulties for patients with low socioeconomic status.ConclusionThe discoursal framing of head and neck cancer treatment may exacerbate inequality because most patients with a low socioeconomic status fail to comprehend and act in accordance with these discourses.
Prognostic significance of CD25 expression in dogs with a noninvasive diagnosis of B‐cell lymphoma treated with CHOP chemotherapy
Authors: Sheng Richard , Clarke Dawn , Laver Travis , Meichner Kristina , Northrup Nicole , Tarigo Jaime 
Published: September 2022

AbstractPrior studies have identified high CD25 expression in canine diffuse large B‐cell lymphoma as a negative prognostic indicator. The objective of this retrospective study was to evaluate CD25 expression as a prognostic indicator in dogs with B‐cell lymphoma (BCL) diagnosed with commonly used noninvasive diagnostics (cytology and flow cytometry [FC]) and treated with CHOP chemotherapy. Lymph node aspirates from 57 dogs with cytologic diagnosis of lymphoma composed of intermediate to large lymphocytes were analysed with FC. Percentage of neoplastic B‐cells expressing CD25 and median fluorescence intensity (MFI) of CD25 were measured. Relationships of CD25 percent positivity and MFI to progression free survival (PFS) and survival time were evaluated. Median survival time (MST) of all dogs was 272 days (95% CI, 196–348 days) and median PFS was 196 days (95% CI, 172–220 days). Higher percentage of B‐cells positive for CD25 was associated with decreased risk of death in multivariable analysis (p = .02). Dogs with higher CD25 positivity had longer MST and PFS than dogs with lower CD25 positivity (318 days versus 176 days and 212 days versus 148 days, respectively), but these differences were not significant. CD25 MFI was not significantly associated with outcome. Based on the results of this study, the association of CD25 expression and prognosis in dogs with BCL diagnosed using noninvasive methods should be interpreted with caution. Further evaluation, with studies that include histopathologic differentiation of lymphoma subtypes, is needed.
Attributes of Bi-Directional Turbomachinery for Pumped Thermal Energy Storage
Authors: Chiapperi J. D. , Greitzer E. M. , Tan C. S. 
Published: September 2022

Abstract
In this paper, we (i) present a methodology for determining the aerodynamic performance of bi-directional turbomachines for pumped thermal energy storage, i.e., turbomachines designed to operate as a compressor in one direction, and then as a turbine in the opposite direction, (ii) carry out performance computations for such turbomachines, and (iii) propose principles for conceptual design of these devices. Focus is placed on using the energy storage cycle not only to identify the novel requirements placed on bi-directional turbomachines but also to estimate the effect of these requirements on the efficiency of the energy storage process. In particular, the difference between aerodynamic loading in forward and in backward operation causes the blading to work at incidences leading to the performance below maximum efficiency, resulting in a lower round-trip efficiency. The description of the design principles includes determination of the number of stages, definition of nondimensional parameters for blading selection, and optimization of two-dimensional blading for bi-directional operation. The assessment of stage count shows the relationship between relative Mach number, pressure ratio, and round-trip efficiency. The nondimensional parameters are assessed through a bi-directional analogue to existing “Smith charts,” for the efficiency of single-direction turbomachines, as a function of camber and stagger. The blade shape evaluation and optimization show how the blade profile can be modified to address the requirements of a bi-directional turbomachine, enabling an increase in round-trip efficiency of two percentage points compared to a baseline double circular arc configuration.
Development of Translucent Design Philosophy for the Cyclic Pattern Design of Fan Outlet Guide Vanes
Authors: Kulkarni Davendu , di Mare Luca 
Published: September 2022

Abstract
The fan systems of typical high bypass civil engines encounter strong flow distortions originating in the intake and the bypass duct. These flow distortions cause the fan stage operation point to vary from its design intent, thus reducing the fan stage performance and increasing low engine-order fan blade forcing. A cyclic pattern design for the fan Outlet Guide Vanes (OGV) can be effectively used to recover the fan stage performance and to control its system-level aeromechanical behavior. This paper presents the development of an OGV pattern design philosophy using the numerical experimentation technique. Multiple fan-intake unsteady computational fluid dynamic computations are conducted by clocking the circumferential pressure profile at the fan exit. The study revealed that a mild, low-harmonic fan back pressure profile with a suitable clocking position is able to improve the fan rotor efficiency and reduce the first engine order (1EO) fan forcing simultaneously. Such a profile can be generated by designing a cyclic OGV pattern that allows the bifurcation potential fields of controlled intensity and phase to pass through the OGV blade row, thus termed the translucent design philosophy. Further, a sensitivity study is performed to assess the effects of simultaneous distortions upstream and downstream of the fan. The study showed that a correctly clocked intra-stage static pressure profile can consistently improve both the performance and aeromechanical behavior of fan systems having different intake lengths and at different flight conditions. The implementation of the proposed translucent design philosophy in a new OGV pattern design tool is discussed.
Numerical Investigation of Near-Tip Modifications for a Highly Loaded Low-Speed Rotor Under the Influence of Double Leakage
Authors: Eckel Jannik , von Jeinsen Philipp , Gümmer Volker 
Published: September 2022

Abstract
This paper numerically investigates the flow field in the tip region of a highly loaded axial low-speed compressor rotor. The rotor in focus is of a hybrid blade configuration, featuring a tandem profile in the mid-span region and single blade profiles near the endwalls. The simulated 1.5-stage configuration also consists of a single row inlet guide vane (IGV) and a tandem stator. Due to high loading coefficients (Ψ = 0.58) in combination with moderate flow coefficients (ϕ = 0.58), double leakage of the tip leakage vortex plays a major role at all operating conditions. As already shown in the past, double leakage is a stability influencing dominant flow phenomenon in compressor rotors and influencing that can be the key to an efficient working range enhancement on compressor stages. This work introduces a simple and powerful method of enhancing the working range for low-speed rotors under the influence of double leakage. It is shown, that modifications to the pressure side (ps) shape of rotor tip profiles can influence the interaction of the tip leakage vortex with the adjacent blade, comparable to the effects of casing treatments. These unconventional blade shapes result from the transformations from tandem blade configurations to hybrid blades near the endwalls. The resulting single blade profile close to the tip features a convex profile element (an increase in local thickness, called the “belly”), which significantly influences the local static pressure gradients. This unusual pressure-side profiling can change mass flow over the tip and, by correct positioning, may reduce the amount of double leakage and, consequently, increase the rotor’s efficient working range. This work presents the geometry definitions for the hybrid blade single-segments, a detailed analysis of the impact on design and off-design operating conditions together with their underlying flow phenomena, and finally proposes design guidelines for the blade-tip geometries of highly loaded rotors under the influence of double leakage.