bluuebunny/crossref_metadata_2025_split huggingface dataset:
ScRNA-seq Expression ofIFI27andAPOC2Identifies Four Alveolar Macrophage Superclusters in Healthy BALF
Authors: Li Xin , Kolling Fred W. , Aridgides Daniel , Mellinger Diane , Ashare Alix , Jakubzick Claudia V. 
Published: January 2022

AbstractAlveolar macrophages (AMs) reside on the luminal surface of the airways and alveoli, ensuring proper gas exchange by ingesting cellular debris and pathogens, and regulating inflammatory responses. Therefore, understanding the heterogeneity and diverse roles played by AMs, interstitial macrophages (IMs), and recruited monocytes is critical for treating airway diseases. We performed single-cell RNA sequencing on 113,213 bronchoalveolar lavage cells from four healthy and three uninflamed cystic fibrosis subjects and identified FOLR2+SELENOP+and SPP1+PLA2G7+IMs, monocyte subtypes, and dendritic cell 1 (DC1), DC2, migDCs, plasmacytoid DCs, lymphocytes, epithelial cells, and four AM superclusters (families) based on the expression ofIFI27andAPOC2genes. These 4 AM families have at least eight distinct functional members (subclusters) named after their differentially expressed gene(s): IGF1, CCL18, CXCL5, Cholesterol, Chemokine, Metallothionein, Interferon and small-cluster AMs. Interestingly, the Chemokine cluster further divides with each subcluster selectively expressing a unique combination of chemokines. One of the most striking observations, besides the heterogeneity, is the conservation of AM family members in relatively equal ratio across all AM superclusters and individuals. Transcriptional data and TotalSeq technology were used to investigate cell surface markers that distinguish resident AMs from recruited monocytes. Lastly, other AM datasets were projected onto our dataset. Similar AM superclusters and functional subclusters were observed, along with changes in AM subclusters in individuals infected with COVID-19. Overall, functional specializations of the AM subclusters suggest that there are highly regulated AM niches with defined programming states, highlighting a clear division of labor.Summary BlurbThere are at least 14 AM subtypes; their frequency, along with other immune cells, are highly conserved across individuals suggesting a specific niche exists for each leukocyte population.Graphical Abstract
A flexible network of lipid droplet associated proteins support embryonic integrity of C. elegans
Authors: Cao Zhe , Fung Chun Wing , Mak Ho Yi 
Published: January 2022

AbstractIn addition to coordinating the storage and mobilization of neutral fat, lipid droplets (LDs) are conserved organelles that can accommodate additional cargos in order to support animal development. However, it is unclear if each type of cargo is matched with a specific subset of LDs. Is the identity of each subset rigidly maintained? Here, we report that SEIP-1/seipin defines a subset of oocyte LDs that are required for proper eggshell formation in C. elegans. Using a photoconvertible fluorescent protein-based imaging assay, we found that SEIP-1 positive LDs were selectively depleted after fertilization, coincident of the formation of a lipid-rich permeability barrier of the eggshell. Loss of SEIP-1 function caused impenetrant embryonic arrest, which could be suppressed by depleting a LD-structural protein PLIN-1/perilipin. The embryonic development of seip-1; plin-1 mutant in turn depended on another LD-associated protein, RAB-18/Rab18. Our results are consistent with the hypothesis that SEIP-1 dependent and independent mechanisms act redundantly to ensure the packaging and export of lipid-rich permeability barrier constituents via LDs. The identity of these LDs, as defined by their associated proteins, exhibits unexpected plasticity that ultimately ensures the survival of embryos ex utero.
Pinpointing the tumor-specific T-cells via TCR clusters
Authors: Goncharov M.M. , Bryushkova E.A. , Sharayev N.I. , Skatova V.D. , Baryshnikova A.M. , Sharonov G.V. , Samoylenko I.V. , Demidov L.V. , Chudakov D.M. , Serebrovskaya E.O. 
Published: January 2022

AbstractAdoptive T cell transfer (ACT) is a promising approach to cancer immunotherapy, but its efficiency fundamentally depends on the extent of tumor-specific T-cell enrichment within the graft. This can be estimated via activation with identifiable neoantigens, tumor-associated antigens (TAAs), or living or lyzed tumor cells, but these approaches remain laborious, time-consuming, and functionally limited, hampering clinical development of ACT. Here, we demonstrate that homology cluster analysis of T cell receptor (TCR) repertoires efficiently identifies tumor-reactive TCRs allowing to: 1) detect their presence within the pool of tumor-infiltrating lymphocytes (TILs); 2) optimize TIL culturing conditions, with IL-2low/IL-21/anti-PD-1 combination showing increased efficiency; 3) investigate surface marker-based enrichment for tumor-targeting T cells in freshly-isolated TILs (enrichment confirmed for CD4+and CD8+PD-1+/CD39+subsets), or re-stimulated TILs (informs on enrichment in 4-1BB-sorted cells). We believe that this approach to the rapid assessment of tumor-specific TCR enrichment should accelerate T cell therapy development.
Thyroid hormones deficiency impairs male germ cell development: a cross talk between hypothalamic-pituitary-thyroid, and - gonadal axes in zebrafish
Authors: Rodrigues Maira S , Tovo-Neto Aldo , da Rosa Ivana F , Doretto Lucas B , Fallah Hamideh P , Habibi Hamid R , Nóbrega Rafael H 
Published: January 2022

AbstractIn vertebrates, thyroid hormones, including thyroxine (T4) and triiodothyronine (T3), are critical players in controlling different physiological processes such as development, growth, metabolism among others. There is evidence in mammals that thyroid hormones are also an important component of the hormonal system that controls reproduction, although studies in fish remain poorly investigated. Here we tested this hypothesis by investigating the effects of methimazole-induced hypothyroidism on the testicular function in adult D. rerio. Treatment of fish with methimazole, in vivo, significantly affected the progression of zebrafish spermatogenesis by inducing the accumulation of pre-meiotic cells, delaying cell differentiation and meiosis, as well as reducing the number of spermatozoa. The observed impairment of spermatogenesis by methimazole was correlated with significant changes in transcript levels for several genes involved in the control of reproduction. Using an in vitro approach, we also demonstrated that in addition to affecting the components of the brain-pituitary-peripheral axis, T3 also exerts direct action at the level of the testis. These results support the hypothesis that thyroid hormones are an essential component of multifactorial control of reproduction and testicular function in zebrafish and possibly other vertebrates.
Hedgehogs are the major amplifying hosts of severe fever with thrombocytopenia syndrome virus
Authors: Zhao Chaoyue , Zhang Xing , Hao Junfeng , Ye Ling , Lawrence Kevin , Lu Yajun , Du Chunhong , Si Xiaoxi , Xu Haidong , Yang Qian , Xia Qianfeng , Yu Guoxiang , Yuan Fei , Jiang Jiafu , Zheng Aihua 
Published: January 2022

AbstractSevere fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne bandavirus mainly transmitted by Haemaphysalis longicornis in East Asia, mostly in rural areas. To date, the amplifying host involved in the natural transmission of SFTSV remains unidentified. Our epidemiological field survey conducted in endemic areas in China showed that hedgehogs were widely distributed, had heavy tick infestations, and had high SFTSV seroprevalence and RNA prevalence. After experimental infection of Erinaceus amurensis and Atelerix albiventris hedgehogs with SFTSV, robust but transitory viremias were detected, which lasted for around nine to eleven days. The infected hedgehogs experienced light weight loss and histopathology of the spleen showed hemorrhagic necrosis and lymphopenia, with infected hedgehogs recovering after viral clearance. Remarkably, SFTSV transmission cycle between hedgehogs and nymph/adult H. longicornis was easily accomplished under laboratory condition with 100% efficiency. Furthermore, naïve H. longicornis ticks could be infected by SFTSV-positive ticks co-feeding on naïve hedgehogs, with transstadial transmission of SFTSV also confirmed. We also found that SFTSV viremia remained high in hedgehogs during hibernation, suggesting that this mechanism might contribute to the persistence of SFTSV from one year to the next. Of concern, we recently found evidence of the natural circulation of SFTSV in the urban area of Beijing City in China involving H. longicornis ticks and E. amurensis hedgehogs. Our study suggests that the hedgehogs are the major wildlife amplifying hosts of SFTSV and that urban outbreaks of SFTSV might occur in the future.
Forced proximity promotes the formation of enduring cooperative relationships in vampire bats
Authors: Razik Imran , Brown Bridget K. G. , Carter Gerald G. 
Published: January 2022

AbstractSpatial assortment can be both a cause and consequence of cooperation. Proximity promotes cooperation when individuals preferentially help nearby partners, and conversely, cooperation drives proximity when individuals move towards more cooperative partners. However, these two causal directions are difficult to distinguish with observational data. Here, we experimentally test if forcing randomly selected pairs of equally familiar female common vampire bats (Desmodus rotundus) into close spatial proximity promotes the formation of enduring cooperative relationships. Over 114 days, we sampled 682 hours of interactions among 21 females captured from three distant sites to track daily allogrooming rates over time. We compared these rates before, during, and after a one-week period, during which we caged random triads of previously unfamiliar and unrelated vampire bats in close proximity. After the week of close proximity when all bats could again freely associate, the allogrooming rates of pairs forced into proximity increased more than those of the 126 control pairs. This work is the first to experimentally demonstrate the causal effect of repeated interactions on cooperative investments in vampire bats. Future work should determine the relative importance of mere association versus interactions (e.g. reciprocal allogrooming) in shaping social preferences.
Clemizole and Trazodone are Effective Antiseizure Treatments in a Zebrafish Model of STXBP1 Disorder
Authors: Moog Maia , Baraban Scott C. 
Published: January 2022

AbstractObjectiveCRISPR-Cas9-generated zebrafish carrying a 12 base-pair deletion in stxbpb1b, a paralog sharing 79% amino acid sequence identity with human, exhibit spontaneous electrographic seizures during larval stages of development. Zebrafish stxbp1b mutants provide an efficient preclinical platform to test antiseizure therapeutics. The present study was designed to test prototype antiepileptic drugs approved for clinical use and two recently identified repurposed drugs with antiseizure activity.MethodsLarval homozygous stxbp1b zebrafish (4 days post-fertilization) were agarose-embedded and monitored for electrographic seizure activity using a local field recording electrode placed in midbrain. Frequency of ictal-like events was evaluated at baseline and following 45 min of continuous drug exposure (1 mM, bath application). Analysis was performed on coded files by an experimenter blinded to drug treatment and genotype.ResultsPhenytoin, valproate, ethosuximide, levetiracetam, and diazepam had no effect on ictal-like event frequency in stxbp1b mutant zebrafish. Clemizole and trazodone decreased ictal-like event frequency in stxbp1b mutant zebrafish by 80% and 83%, respectively. These results suggest that repurposed drugs with serotonin receptor binding affinities could be effective antiseizure treatments.SignificanceClemizole and trazodone were identified in a larval zebrafish model for Dravet syndrome. Based primarily on these preclinical zebrafish studies, compassionate-use and double-blind clinical trials with both drugs have progressed. The present study extends this approach to a preclinical zebrafish model representing STXBP1-related disorders, and suggests that future clinical studies may be warranted.
NCOA4 drives ferritin phase separation to facilitate macroferritinophagy and endosomal microferritinophagy
Authors: Ohshima Tomoko , Yamamoto Hayashi , Sakamaki Yuriko , Saito Chieko , Mizushima Noboru 
Published: January 2022

AbstractA ferritin particle consists of 24 ferritin proteins (FTH1 and FTL) and stores iron ions within it. During iron deficiency, ferritin particles are transported to lysosomes to release iron ions. Two transport pathways have been reported: macroautophagy and ESCRT-dependent endosomal microautophagy. Although the membrane dynamics of these pathways differ, both require NCOA4, which is thought to be an autophagy receptor for ferritin. However, the exact function of NCOA4 remains elusive. Here, we found that ferritin particles form liquid-like condensates in a NCOA4-dependent manner. Homodimerization of NCOA4 and interaction between FTH1 and NCOA4 (i.e., multivalent interactions between ferritin particles and NCOA4) were required for the formation of ferritin condensates. Disruption of these interactions impaired ferritin degradation. Time-lapse imaging and three-dimensional correlative light and electron microscopy revealed that these ferritin–NCOA4 condensates were directly engulfed by autophagosomes and endosomes. In contrast, TAX1BP1 was not required for the formation of ferritin–NCOA4 condensates but was required for their incorporation into autophagosomes and endosomes. These results suggest that NCOA4 acts not only as a canonical autophagy receptor but also as a driver to form ferritin condensates to facilitate the degradation of these condensates by macroautophagy (i.e., macroferritinophagy) and endosomal microautophagy (i.e., microferritinophagy).
An evolutionary structural optimization algorithm for the analysis of light automobile parts using a meshless technique
Authors: Gonçalves Diogo , Lopes Joel , Campilho Raul , Belinha Jorge 
Published: January 2022

PurposeThe purpose of the present work is to develop the combination of the radial point interpolation method (RPIM) with a bi-directional evolutionary structural optimization (BESO) algorithm and extend it to the analysis of benchmark examples and automotive industry applications.Design/methodology/approachA BESO algorithm capable of detecting variations in the stress level of the structure, and thus respond to those changes by reinforcing the solid material, is developed. A meshless method, the RPIM, is used to iteratively obtain the stress field. The obtained optimal topologies are then recreated and numerically analyzed to validate its proficiency.FindingsThe proposed algorithm is capable to achieve accurate benchmark material distributions. Implementation of the BESO algorithm combined with the RPIM allows developing innovative lightweight automotive structures with increased performance.Research limitations/implicationsComputational cost of the topology optimization analysis is constrained by the nodal density discretizing the problem domain. Topology optimization solutions are usually complex, whereby they must be fabricated by additive manufacturing techniques and experimentally validated.Practical implicationsIn automotive industry, fuel consumption, carbon emissions and vehicle performance is influenced by structure weight. Therefore, implementation of accurate topology optimization algorithms to design lightweight (cost-efficient) components will be an asset in industry.Originality/valueMeshless methods applications in topology optimization are not as widespread as the finite element method (FEM). Therefore, this work enhances the state-of-the-art of meshless methods and demonstrates the suitability of the RPIM to solve topology optimization problems. Innovative lightweight automotive structures are developed using the proposed methodology.
Pricing research in hospitality and tourism and marketing literature: a systematic review and research agenda
Authors: Han Wenjia , Bai Billy 
Published: January 2022

PurposeThis study systematically reviewed pricing research published in leading marketing and hospitality and tourism (H&T) journals between 2010 and 2019. It attempts to concretize the understanding of the evolving patterns of pricing research in both fields and suggests an agenda for future research in H&T.Design/methodology/approachThis study performed keyword co-occurrence analyses and co-citation analyses on the bibliographic data of 575 articles from marketing and H&T journals. Content analysis was applied to investigate the emerged topics in H&T.FindingsThe marketing discipline showed a persistent focus on research themes including price promotion, reference price, price fairness and pricing strategy. The H&T domain experienced a significant content enrichment of preexistent research topics. H&T scholars showed a growing interest in studying pricing for sharing economy accommodations and the interplay between pricing and electronic word-of-mouth. While marketing research applied theories from multiple disciplines as the theoretical foundations, H&T studies adopted the hedonic pricing model as an overarching theory.Practical implicationsFuture pricing research in H&T may incorporate theories from other disciplines such as psychology, sociology and anthropology to broaden the scope of the study. Besides, innovative study designs and complex data analysis techniques should be encouraged. Topicwise, H&T scholars can dive deeper into price promotion and distribution channel price management.Originality/valueThis is the first study consolidating the pricing literature in H&T and marketing. It also suggests the potential research directions for researchers.
Numerical study of aerodynamic performance and flow characteristics of a centrifugal blower
Authors: Le Thanh-Long , Nghia Tran Trung , Thong Hong Duc , Son Mai Hoang Kim 
Published: January 2022

PurposeThis paper aims to focus on the effect of the operating condition such as the impeller speed on the centrifugal fan performance and flow characteristics. The ability to predict the behavior of the airflow motion in a centrifugal blower is essential for obtaining the topology optimization design.Design/methodology/approachA physical model of the air blower consisting of these main parts in a blower system: collector, impeller, outlet flange and volute casing, and the appropriate boundary conditions are set up by ANSYS software. Computation fluid dynamics are performed for the numerical analysis. The calculation of blower performance parameters such as total pressure, efficiency and flow rate is based on the Reynolds averaged Navier–Stokes equations and k-εturbulence flow model.FindingsThe numerical results show that the change in operating conditions has a significant effect on the blower performance, and the pressure maintained inside the blower is higher for a larger impeller rotational speed.Originality/valueThis work is original and has not yet been submitted to elsewhere or published previously.
Exploring the multi-level influence of shared leadership on workplace spirituality in teams
Authors: Prabhu Nandan , Modem Roopa 
Published: January 2022

Purpose
This study aims to examine the association between shared transformational leadership and workplace spirituality in teams. While articulating its theoretical propositions, this research draws on the theory of motivational effects of charismatic leadership based on the self-concept.

Design/methodology/approach
The paper has adopted the quantitative method and cross-sectional research design while examining the study’s variables’ multi-level relationship. The authors researched 141 project teams in India’s information technology setting.

Findings
This study shows that shared transformational leadership is associated with workplace spirituality. This research has also found significant within-team variance in team members’ workplace spirituality experiences.

Practical implications
The study’s results show that the information technology companies’ employees aspire to cultivate individual-specific meaning at work. Therefore, team leaders should strive to display transformational leadership behaviours to build individual-specific meaning and a sense of community among team members.

Social implications
The study’s findings imply that shared transformational leadership can reduce individualistic utilitarianism and the resulting baneful impact of hedonism. Society can also benefit from shared leadership’s impact on individuals’ self-work integration as it will probably strengthen the “other-benefitting” behaviour instead of mere hedonistic orientation.

Originality/value
This paper has addressed the theoretical tensions regarding the role of shared transformational leadership in inducing employees’ inner life, meaning at work and sense of community. This paper helps us understand shared transformational leadership’s effects on individual workplace spirituality experiences.
Commodity prices, inflation and inflation uncertainty among emerging economies
Authors: Abaidoo Rexford , Agyapong Elvis Kwame 
Published: January 2022

PurposeThis paper examines the role price fluctuations associated with internationally traded commodities play in inflationary conditions and inflation uncertainty among economies in Sub-Saharan Africa.Design/methodology/approachUsing a panel 32 countries from the sub-region from 1996 to 2019, this study employed Two-Step System Generalized Method of Moments (GMM) estimation technique in its analysis.FindingsEmpirical evidence demonstrates that fluctuations in forex-adjusted price of crude oil, gold and cocoa have significant positive impact on inflation while forex-adjusted changes in price of cotton tend to have significant negative influence on consumer price inflation among economies in the sub-region. Additionally, the study found that gold, cocoa and cotton price changes on the international market have significant positive impact on inflation uncertainty in the sub-region (rise in price leads to increase rate of inflation uncertainty). Furthermore, improved regulatory quality and growth in output growth (GDP per capita growth) were found to help in stabilizing inflation uncertainty (reduce inflation uncertainty) among economies in the sub-region during periods of persistent growth in general price levels.Originality/valueThe study present a different approach based on individual economy forex-adjusted global prices of internationally traded commodities instead of general prices often used in the literature and assessed the effects such adjusted commodity prices have on inflation and inflation uncertainty. Additionally, the moderating role of regulatory quality and output growth between surmised nexuses are also examined.