bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isorhamnetin improves cognitive impairment of Alzheimer’s disease through Sirt1/AKT/ERK/CREB signaling pathway
Authors: Ying Li , Rong-Wei Zhang , Sha Sha 
Published: June 2022

Abstract
Isorhamnetin (IH) is one of a flavonoid with extensive pharmacological activities, including anti-inflammation and antioxidation. Although IH has a protective effect in neurological diseases, its role in Alzheimer's disease (AD) is not very clear. In order to explore the effect and mechanism of IH in AD, 4-month-old APP/PS1mice treated with IH in 50 mg/kg/d for 3 months and Morris water maze and immunofluorescence staining were used to detect whether IH could improve AD pathology. The mechanisms of IH improving AD pathology were detected by Western blot. The results showed that after IH treatment, the time of escape latency was significantly reduced and the number of crossing the platform was significantly increased, suggesting that IH can improve the cognitive impairment of mice. Moreover, IH significantly decreased senile plaques deposition in the brain and increased the expression of ADAM10 in APP/PS1 mice through activating Sirt1/AKT/ERK/CREB pathway. Furthermore, IH reduced ROS formation, GP91 expression, upregulated the expression of SOD1 and Nrf2. Moreover, IH inhibited astrocytes activation and pro-inflammatory cytokine IL-1β and TNF-α release. In conclusion, IH improved cognitive function, inhibited oxidative stress and inflammation response in AD. Therefore, we suggest that IH may be considered as a potential drug for AD treatment.
Evaluation of Optimum Thickness of Insulation Materials and Their Carbon Mitigation Potential in Indian Climatic Zone
Authors: Mahapatra Debasish , Madav Vasudeva , Setty Ashok Babu Talanki Puttaranga 
Published: June 2022

Abstract
Buildings consume an enormous amount of energy and are responsible for emitting massive carbon dioxide. About 33% of total energy is consumed for achieving thermal comfort in buildings. Although building insulation is a sustainable and economical option for passive cooling and guarantees long-term return, its use is still minimal in India. Only 4% of building out of all green-rated buildings are residential In India. In this study, optimum insulation thickness of cellulose, corn-based insulation, polyurethane, and polystyrene when used with different plastered brick materials having different absorptivity of the external surface is evaluated using the life cycle costing method. Four cities of India, such as Delhi, Jodhpur, Mangalore and Pune, are considered for this study to cover all the climatic zones of India. The cost-saving that can be achieved by using optimum insulation thickness is in the range of 15.6$/m2-133.3$/m2, 23.5$/m2-171.2$/m2, 15.9$/m2-147.4$/m2 and 5.7$/m2-106.3$/m2 in Delhi, Jodhpur, Mangalore and Pune, respectively. The carbon mitigation potential of optimum insulations is in the range of 22.9 kg/kWh-136.5 kg/kWh, 32.2 kg/kWh − 173.9 kg/kWh, 23.1 kg/kWh − 150.4 kg/kWh, 10.2 kg/kWh − 109.6 kg/kWh in Delhi, Jodhpur, Mangalore and Pune respectively. This study will help create awareness about the cost-saving potential of insulation, thereby increasing its use, which will help India achieve the vision of creating a 10 billion square feet green built-up area.
Brain network mechanism of working memory impairment in temporal lobe epilepsy: A resting-state fMRI study
Authors: Zhang Xin , Li Yuying , Bao Jie , Guo Dandan , Zhang Lulin , Pan Liping , Yin Jianzhong , Song Yijun 
Published: June 2022

Abstract
Background: Most temporal lobe epilepsy patients accompany with working memory impairment, but the specific brain network mechanism is still unclear. In this study, we used independent component analysis (ICA) to segregate resting-state magnetic resonance imaging (MRI) and evaluated the brain network mechanism of working memory impairment in patients with temporal lobe epilepsy (TLE). Methods: Resting-state functional magnetic resonance imaging (fMRI) data were collected from 38 patients with TLE and 20 healthy controls. TLE patients were divided into a disordered working memory group (TLE-DWM) and a non-disordered working memory group (TLE-NWM) according to their performance in a delayed matching-to-sample (DMS) working memory task. We used ICA to extract the default mode network (DMN), left central executive network (LCEN), right central executive network (RCEN), and salience network (SN), and to compare the functional connection changes between the four groups. Pearson correlation analysis was used to calculate the correlation between connection strength and working memory performance. Results: intra-network, inter-network, DMS working test, pearson correlation.We found that the connectivity of the right inferior parietal lobe and precuneus lobe in the control and TLE-NWM groups was higher than that in the TLE-DWM group. The connectivity of the posterior cingulate gyrus in the control and TLE-NWM groups was higher than that in the TLE-DWM group. The connection of the putamen in the control and TLE-NWM groups was greater than that in the TLE-DWM group. The Z values of the TLE-DWM, TLE-NWM, and control groups in the posterior cingulate gyrus of the LCEN network were negatively correlated with response time (RT). Conclusion: This study demonstrated that the functional connectivity of right inferior lobe, left precuneus lobe, left posterior cingulate gyrus, left putamen, and DMN-LECN network was decreased in TLE-DWM individuals, indicating that these network alternations correlated with the working memory impairment. In addition, we also found the connectivity of the posterior cingulate gyrus in LECN was negatively correlated with RT, which suggested that posterior cingulate gyrus was associated with the performance of working memory.
Compressive strength characteristics and mechanism of tungsten tailings activated by multi concentration alkali
Authors: Li Shanmei , Shou Kai , Wang Lei , Liu Zhikui 
Published: June 2022

Abstract
The 7d unconfined compressive strength tests of alkali-activated tungsten tailings and the microscopic characteristics tests of scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) were conducted to investigate the variation law and mechanism of unconfined compressive strength of alkali-activated tungsten tailings under the conditions of seven alkali-solid ratios with the same moisture content. The test results indicate that the unconfined compressive strength of alkali-activated tungsten tailings is increased with the alkali-solid ratio. However, the strength decreases slightly when the alkali-solid ratio is 12%. Alkali activation does not change the crystal type on the micro-scale but forms gels including C-A-S-H and N-A-S-H. The microstructures of the gel are affected by the alkali-solid ratio: The microstructure is honeycomb in low alkali-solid ratio (7%, 8% and 10%), with N-A-S-H as its primary form, and flocculation in high alkali-solid ratio (14% and 15%), mainly in the form of C-A-S-H. When the solid alkali ratio is at the medium level (12%), the microstructure is a small round bead, and the N-A-S-H is equivalent to the C-A-S-H. The generation of C-A-S-H and N-A-S-H gels is the key to increase the strength of alkali activated tungsten tailings. The more C-A-S-H content, the greater the strength. This study can provide a scientific basis and technical reference for the resource utilization of tungsten tailings.
WITHDRAWN: The CO2 emissions and financial development relationship study-based on the environmental pollution background
Authors: Duan Keyi , Cao Mingyao , Malim Nurhafiza Abdul Kader 
Published: June 2022

Abstract
In recent years, environmental pollution has caused many problems, especially the CO2 emission. The rapid rise of industrialization and urbanization has had a significant detrimental influence on the environment, according to the World Health Organization's 2011 "Global Air Quality Report," around 2 million people die each year as a result of air pollution. People's physical health is adversely harmed by illnesses, the majority of which occur in developing countries. Achieving the coordinated development of financial development and environmental protection has become a social hotspot. Based on panel data including 30 provinces in China from 2000 to 2020, this paper employs the panel smooth transition regression (PSTR) model to examine the impact of financial development on carbon dioxide emissions. The results indicate that financial development has a nonlinear effect on carbon dioxide emissions. Furthermore, the positive effect of financial development on carbon dioxide emissions is through scale effect and structural effect, especially the latter effect is responsible for up to 183.98%, while the negative effect is through technological effect, which has more space. Moreover, the financial added value, financial scale, financial efficiency, and foreign direct investment are adopted to measure financial development, which the first two indicators show a smooth transition, the other two indexes always show a positive influence.
Inclusion of calcium oxide in sugarcane silage with different particle size in diet of F1 Holstein / Zebu dairy cows: nutritional and productive parameters
Authors: Andrade Weudes Rodrigues , Machado Thiara Jacira Vicuna Mendes Oliveira de Paula , DAVID GABRIEL SANTOS , Pires Aureliano José Vieira , de Carvalho Gleidson Giordano Pinto , Martins Susi Cristina dos Santos Guimarães , de Sousa Luiz Carlos Oliveira 
Published: June 2022

Abstract
The aim was to evaluate intake and apparent digestibility, productive performance and nitrogen balance of the F1 Holstein/Zebu dairy cows fed sugarcane silage with different particle sizes containing or not calcium oxide (CaO). Eight F1 Holstein/Zebu cows, with average body weight of 521.5 ± 55.17 kg, and 60 ± 10 days in milk were used, distributed in two simultaneous 4x4 Latin squares. The treatments were composed of sugarcane in two particle sizes (15 and 30 mm) with and without addition of CaO (10 g/kg natural matter basis), being the treatments compared according to a 2 x 2 factorial arrangement. Data were analyzed by analysis of variance using the MIXED procedure of SAS (version 9.4). The intakes of the dry matter (13.05 kg/d), crude protein, non-fibrous carbohydrates and neutral detergent fiber were not modified (P > 0.05) by the inclusion of the calcium oxide, nor ot by particles size and interaction among both. However, there was interaction between CaO and particle size on dry matter digestibility (P = 0.002). The yield (13.85 kg/d) and composition of the milk were also not affected by diets (P > 0.05), similarly for nitrogen balance (P > 0,05). The addition of CaO to sugarcane silage with different particle sizes (15 and 30 mm) does not affect the yield and composition of the milk of Holstein/Zebu cows, nor the nitrogen balance. However, when CaO is added to sugarcane silage with reduced particle size, there is a loss in dry matter digestibility.
Ex vivo Expanded Human Regulatory T Cells Modify Neuroinflammation in a Preclinical Model of Alzheimer’s disease
Authors: Faridar Alireza , Vasquez Matthew , Thome Aaron D. , Yin Zheng , Xuan Hui , Wang Jing Hong , Wen Shixiang , Li Xuping , Thonhoff Jason R. , Zhao Weihau , Zhao Hong , Beers David R. , Wong Stephen T.C. , Masdeu Joseph C. , Appel Stanley 
Published: June 2022

Abstract
Background:
Regulatory T cells (Tregs) play a neuroprotective role by suppressing microglia and macrophage-mediated inflammation and modulating adaptive immune reactions. We previously documented that Treg immunomodulatory mechanisms are compromised in Alzheimer’s disease (AD). Ex vivo expansion of Tregs restores and amplifies their immunosuppressive functions in vitro. A key question is whether adoptive transfer of ex vivo expanded human Tregs can suppress neuroinflammation and amyloid pathology in a preclinical mouse model.
Methods:
An immunodeficient mouse model of AD was generated by backcrossing the 5xFAD onto Rag2 knockout mice (5xFAD-Rag2KO). Human Tregs were expanded ex vivo for 24 days and administered to 5xFAD-Rag2KO. Changes in amyloid burden, microglia characteristics and reactive astrocytes were evaluated using ELISA and confocal microscopy. NanoString Mouse AD multiplex gene expression analysis was applied to explore the impact of ex vivo expanded Tregs on the neuroinflammation transcriptome.
Results:
Elimination of mature B and T lymphocytes and natural killer cells in 5xFAD-Rag2KO mice was associated with upregulation of 95 inflammation genes and amplified number of reactive microglia within the dentate gyrus. Following peripheral administration, ex vivo expanded human Tregs were detectable in the frontal cortex and dentate gyrus of 5xFAD-Rag2KO and reduced amyloid burden and reactive glial cells. Interrogation of inflammation gene expression documented down-regulation of pro-inflammatory cytokines (IL1A&B, IL6), complement cascade (C1qa, C1qb, C1qc, C4a/b), toll-like receptors (Tlr3, Tlr4 and Tlr7) and microglial activations markers (CD14, Tyrobp,Trem2).
Conclusions:
Ex vivo expanded human Tregs, with amplified immunomodulatory function, suppressed neuroinflammation and alleviated AD pathology in vivo. Our results provide preclinical evidences for Treg cell therapy as a potential treatment strategy in AD.
Integrated use of well logging and geophysical data to define stratigraphy on a contaminated urban site in São Paulo, Brazil.
Authors: Netto Leonides Guireli , Barbosa Alexandre Muselli , Pereira João Pedro Silva , Gandolfo Otávio Coaracy Brasil , Guimarães Camila Camolesi , Birelli Carlos Alberto , de Freitas Leandro Gomes , Nascimento Claudio Augusto Oller do 
Published: June 2022

Abstract
One of the most important steps in the environmental remediation of contaminated areas is the stratigraphic characterization of the study area. The behavior of the contaminant in the environment is directly related to the lithological composition and hydrogeological factors of the stratigraphic layers. In the present study, geoenvironmental, geotechnical and geophysical investigations were applied in an old wood treatment area close to one of the main rivers in the city of São Paulo, Brazil. The use of geophysics in this scenario resulted in information gain in relation to drilling tests, which are strictly punctual. Geophysical methods not commonly applied in environmental studies were used, associated with high resolution geoenvironmental investigation techniques, which allowed a detailed characterization of the the study area stratigraphy. Geophysical (MASW and gamma well logging) and geoenvironmental (electrical conductivity profiles) data were compared with granulometric variations in soil characterization laboratory tests. The results showed that the association of invasive and non-invasive investigation techniques is a viable alternative in studies of stratigraphic characterization of contaminated areas, mainly in tropical environments.
Effects of music therapy as an alternative treatment on depression in children and adolescents with ADHD by activating serotonin and improving stress coping ability
Authors: Park Jong-In , Lee In-Ho , Kwon Ryeo-Won , Choo Eon-ah , Lee Jeong-Beom 
Published: June 2022

Abstract
BackgroundThe objective of this study was conducted to determine the effect of music therapy as an alternative treatment on depression in children and adolescents with attention-deficit hyperactivity disorder (ADHD) by activating serotonin (5-HT) and improving stress coping ability. Methods A total of 43 subjects participated in the experiment, consisting of an ADHD group (n = 18, age of 12.15 ± 2.43 years; height of 142.57 ± 9.98 cm; weight of 41.45 ± 13.87 kg) and a control group (n = 25, with age, height, and weight similar to those with ADHD). Music therapy was regularly performed for all subjects twice a week for three months, 50 minutes each, for a total of 24 times. From a neurophysiological perspective, changes in depression and stress were tracked by measuring 5-HT secretion, cortisol expression, blood pressure (BP), heart rate (HR), and psychological scales. Results As a result of the measurement, in both ADHD group and control groups, 5-HT secretion was increased (p < 0.001), whereas cortisol expression (p < 0.001), BP (p < 0.05), and HR (p < 0.001) were decreased after music therapy. The psychological scale also showed positive changes (p < 0.01). ConclusionsIn conclusion, the application of music therapy as an alternative treatment for ADHD children and adolescents showed positive neurophysiological and psychological effects. Therefore, this study would like to propose a new alternative of medicine for preventing and treating depression through various uses of music therapy.
B cell receptor (BCR) diversity and differential CDR3s usage as potential immune indictors of diffuse large B cell lymphoma (DLBCL) Running title: Vital roles of BCR traits in DLBCL
Authors: Jiang Wenhua , Zhou Shiyong , Li Jian , Zhu Guoqing , Gao Mingyou , Zhao Kuo , Zhang Limeng , Xie Xiaojing , Zhao Ning , Tian Caijuan , Zhang Zhenzhen , Zhao Yanpeng , Guo Yixian , Cui Yunlong , Liu Pengfei 
Published: June 2022

Abstract
The aim of the present study was to characterize DLBCL of BCR distributions, and to screen specific BCR features as potential indicators related to DLBCL prognosis. A total of 13 patients with DLBCL and 5 healthy individuals were enrolled in the present study. In total, two high throughput sequencing methods, BCR repertoire and WES, were used to reveal BCR distributions and gene mutations, respectively. A series of comprehensive analyses were conducted to identify potential complementarity determining region 3 (CDR3) indicators of DLBCL and to analyze their association with clinical characteristics. The relatively higher BCR diversity in patients with DLBCL was associated with shorter progression-free survival (PFS). In addition, the top 10 differential CDR3s were screened as potential DLBCL indicators, and among these, four CDR3s [immunoglobulin heavy chain IGHV1-8/IGHJ6, IGHV3-74/IGHJ6, IGHV4-39/IGHJ3 and IGHV4-34/IGHJ4] exhibited a close relationship with PFS in patients with DLBCL. In further analysis, IGHV4-34/IGHJ4 and IGHV4-39/IGHJ3 were identified to be directly related to tumor metastasis, and IGHV4-39/IGHJ4 was related to hepatitis B virus (HBV) infection. WES data were used to analyze the gene mutations in patients with DLBCL, indicating that two metastasis pathways (‘focal adhesion’ and ‘ECM-receptor interaction’) were involved in characteristic changes of BCR. In conclusion, BCR diversity and usage of 10 specific CDR3s were identified as potential DLBCL indicators in the present study. Clinically, inflammation and HBV infection were the two main factors associated with changes of BCR characteristics. In terms of the molecular mechanism, BCR distributions were closely associated with metastasis-related pathways.
Development of an AI and IoT Driven Decision Support System for Sheep Breeding Farms
Authors: Hamadani Ambreen , Ganai Nazir A. 
Published: June 2022

Abstract
The science of animal breeding is based on the use of statistical designs and genetic models. A fundamental requirement, however, is the availability of accurate and reliable pedigreed data and tools facilitating sophisticated computations. The sheep husbandry sector in India has been quite unable to tap into the potential of either. As the global challenges of food insecurity and population explosion become more pressing, there is a dire need to revamp the existing breeding and farm management systems. To address this challenge, farming practices must evolve to become a part of the technological revolution. Keeping this in view, Smart Sheep Breeder (SSB) – a fully functional multi-use online Artificial Intelligence and Internet of Things -enabled Decision Support System (DSS) for automatic performance recording, farm data management, data mining, biometrical analysis, e-governance, decision-making in sheep farms was developed. This mega database thus developed would be capable of across farm genetic ranking of sheep and effective dissemination of germplasm. The first-of-its-kind tool in India is available as a web-based tool and android application which facilitates performance recording in animals in simple-to-use data entry e-forms and generate customized reports on various aspects of sheep production. SSB uses artificial intelligence and world class biometrical genetic algorithms to calculate breeding values, and inbreeding coefficients, construct selection indices and generate pedigree, history sheets as well as more than 40 types of custom-tailored animal and farm reports and graphs. The algorithms used were validated using farm data and comparison with established methods. Smart Sheep Breeder could thus prove to be indispensable for the present farming systems which could subsequently be used by breeders across India.
A neuroprotective role of Ufmylation through Atg9 in the aging brain of Drosophila
Authors: Li Huifang , Wei Zhenhao , Yu Zhenghong , Cai Yafei , Ma Fei , Cheng Yun , Zhang Wei 
Published: June 2022

Abstract
Ufmylation is a recently identified small ubiquitin-like modification, whose biological function and relevant cellular targets are poorly understood. Here we present evidence of a neuroprotective role for Ufmylation involving Autophagy-related gene 9 (Atg9) in the aging brain of Drosophila. The Ufm1 system ensures the health of aged neurons via Atg9 by coordinating autophagy and mTORC1, and maintaining mitochondrial homeostasis and JNK (c-Jun N-terminal kinase) activity. Neuron-specific expression of Atg9 suppresses the age-associated movement defect and lethality caused by loss of Ufmylation. Furthermore, Atg9 is identified as a conserved target of Ufm1 conjugation mediated by Ddrgk1, a critical regulator of Ufmylation. Mammalian Ddrgk1 was shown to be indispensable for the stability of endogenous Atg9A protein in mouse embryonic fibroblast (MEF) cells. Taken together, our findings might have important implications for neurodegenerative diseases in mammals.
Localized phosphorus promotes nutrient productivity of Brassica chinensis genotype with strong root morphological plasticity
Authors: Gao Wei , Zhang Deshan , Tang Xiaoyan , Gao Xiang , Ruan Yunze , Zhang Yuqiang , Shen Jianbo 
Published: June 2022

Abstract
Aims
Crops adjust root morphological and/or exudation traits in response to phosphorus (P) availability and heterogeneous-supplied P, governing crop productivity. Yet, how the intraspecific plasticity of root P-acquisition strategies influence plant adaptation to localized P fertilization across doses remains obscure.
Methods
Root P-acquisition traits were assessed in Brassica chinensis L. Xiaqing and Yanchun grown in soil with four mineral P fertilization levels (0, 15, 24, and 30 kg P ha− 1), and with broadcast and localized P across two levels (15 and 24 kg P ha− 1) to characterize efficient strategies in relation to large plant P productivity.
Results
Genotype of Xiaqing stimulated growth of roots with large specific length and increased exudation of acid phosphatase and carboxylates under 15 kg P ha− 1 fertilization as compared to those in 24 and 30 kg P ha− 1 soil. Conversely, Yanchun increased root elongation in 15 kg P ha− 1 soil compared with crops in soil fertilizing 30 kg P ha− 1. Xiaqing and Yanchun stimulated root proliferation in P-rich soil volume with 15 and 24 kg P ha− 1, but with longer roots in the former than the later genotype. Localized P at 15 kg P ha− 1 level, compared with 24 kg P ha− 1, increased root length in patchy soil zone and promoted P acquisition in Xiaqing and Yanchun.
Conclusion
Genotype of Xiaqing exhibited strong root plasticity in response to low and localized P application compared with Yanchun. Nutrient-efficient genotype coupling with localized fertilization was important to increase crop yield, saving fertilizer in vegetable cropping system.