bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bortezomib inhibits the proteasome, leading to cell death via apoptosis in feline injection site sarcoma cells in vitro
Authors: Laver Travis , Lee Benjamin M. , Gogal Robert M. 
Published: May 2022

Abstract

OBJECTIVE
To determine the in vitro effects of the proteasome inhibitor bortezomib in feline injection site sarcoma (FISS) cell lines.

SAMPLE
In vitro cultures of the FISS cell lines Ela-1, Hamilton, and Kaiser.

PROCEDURES
Cells were treated with increasing doses of bortezomib or vehicle alone (dimethyl sulfoxide) and evaluated for cell viability via an adenosine triphosphate concentration assay, proteasome activity via a commercially available proteasome assay, accumulation of ubiquitinated proteins via Western blot, and apoptosis via flow cytometry.

RESULTS
All 3 cell lines were sensitive to bortezomib with a 50% inhibitory concentration after 48 hours of treatment at 17.46 nM (95% CI, 15.47 to 19.72 nM) for Ela-1, 19.48 nM (95% CI, 16.52 to 23.00 nM) for Hamilton, and 21.38 nM (95% CI, 19.24 to 23.78 nM) for Kaiser. In the Ela-1 cell line, 20 nM bortezomib inhibited 20S proteasome activity by 90.9% compared with the vehicle-only control. In the Kaiser cell line, 20 nM bortezomib decreased 20S proteasome activity by 70%, compared with the untreated vehicle-only control. Last, treatment with bortezomib (25 and 40 nM) resulted in statistically significant decreases in viable cells accompanied by a statistically significant increase in apoptotic cells.

CLINICAL RELEVANCE
Treatment options for FISS, especially nonresectable FISS, are currently very limited. These results support further investigation of bortezomib either alone or in combination with other treatments in such cases.
High-dose buprenorphine results in a greater occurrence of postoperative hyperthermia than morphine in a randomized clinical trial in cats undergoing ovariohysterectomy
Authors: Martin-Flores Manuel , Lorenzutti Augusto M. , Nugen Sarah A. , Boesch Jordyn M. , Kirch Pati J. , Merry Hillary L. , Campoy Luis 
Published: May 2022

Abstract

OBJECTIVE
To compare the thermoregulatory and analgesic effects of high-dose buprenorphine versus morphine in cats undergoing ovariohysterectomy.

ANIMALS
94 client-owned cats.

PROCEDURES
Cats were randomized to receive either buprenorphine 0.24 mg/kg or morphine 0.1 mg/kg subcutaneously (SC) during recovery from ovariohysterectomy. Body temperature measurements were obtained before anesthesia, during anesthesia (averaged), at extubation, and 2, 4, and 16 to 20 hours postoperatively. Signs of pain were assessed, and demographic characteristics were compared between groups. The effects of treatment and time on body temperature, point prevalence of hyperthermia (> 39.2 °C), and pain scores were compared with linear or generalized mixed-effect models.

RESULTS
Cats receiving morphine (vs. buprenorphine) were older and heavier (both, P ≤ 0.005). Other group characteristics did not differ between treatments. Cats receiving buprenorphine (vs. morphine) had higher postoperative temperatures (P = 0.03). At 2, 4, and 16 to 20 hours after extubation, the point prevalence of hyperthermia was greater (P = 0.001) for cats receiving buprenorphine (55% [26/47], 44% [21/47], and 62% [27/43], respectively) versus morphine (28% [13/46], 13% [6/46], and 47% [21/44], respectively). There were no differences in pain scores between groups or over time. Five cats receiving buprenorphine and 6 receiving morphine required rescue analgesia within the 24-hour period.

CLINICAL RELEVANCE
Administration of buprenorphine (0.24 mg/kg SC), compared with morphine (0.1 mg/kg SC), resulted in higher body temperatures without an apparent advantage with regard to analgesia during the first 20 postoperative hours than morphine. Opioid-induced postoperative hyperthermia could confound the diagnosis of fever from different sources.
Transpulmonary ultrasound dilution is an acceptable technique for cardiac output measurement in anesthetized pigs
Authors: Paranjape Vaidehi V. , Shih Andre C. , Garcia-Pereira Fernando L. , Saksena Siddharth 
Published: May 2022

Abstract

OBJECTIVE
To evaluate cardiac output (CO) measurements using transpulmonary ultrasound (TPUD) technology and compare results with those of the gold standard, pulmonary arterial catheter thermodilution (PACTD), in 6 healthy anesthetized pigs during acute hemodynamic changes caused by manipulation of the blood volume.

ANIMALS
6 healthy male Landrace pigs.

PROCEDURES
Over a period of 1 week, pigs were anesthetized with isoflurane, mechanically ventilated, and underwent instrumentation in dorsal recumbency. They were subjected to sequential experimental states during which the blood volume was manipulated so that the animals transitioned from normovolemia to hypovolemia (20% and 40% of blood volume depletion), back to normovolemia (autologous blood transfusion), and then to hypervolemia (following colloid bolus). During each volume state, CO measurements were compared between TPUD and PACTD.

RESULTS
The mean ± SD relative bias between TPUD and PACTD was 7.71% ± 21.2% with limits of agreement –33.9% to 49.3%, indicating TPUD slightly underestimated CO values, compared with values obtained with PACTD. The mean ± SD of the bias between the 2 methods was 0.13 ± 0.5 L/min. Only 5 of 36 (13.9%) TPUD CO measurements had an absolute value of relative bias > 30%. The percentage error calculated for TPUD was 29.4%.

CLINICAL RELEVANCE
Results suggested that TPUD measurements have acceptable agreement with PACTD measurements. Moreover, TPUD exhibits promising potential in being used interchangeably with PACTD for future hemodynamic research involving swine as species of interest.
Nutrition and health functionality of hemp products in wheat cereal products – butter cut-out biscuits and leavened pastry
Authors: Hrušková Marie , Švec Ivan 
Published: May 2022

Abstract
Native to China, hemp (Cannabis sativa L.) is an annual herb whose anatomical parts are widely used by for technical, food, and medical purposes. Hemp is traditionally considered a controversial crop due to its cannabinoid content. For industrial and food usage, however, the content of psychoactive tetrahydroxycannabinol (known as THC) is limited by EU Regulation (0.2%). In some countries, including the Czech Republic, the supportive and therapeutic effect of THC has led to the legalization of controlled cultivation (Elkoplast Slušovice, hemp with 6–19% THC) and the use of hemp on prescription. Due to the high content of proteins, fats, and fibre in hemp seeds, they are suitable for increasing the nutritional value of food. For cereal products, it is recommended to use wholemeal or defatted flour in dosages limited by the sensory quality of the final products, e.g., up to 10% for pastry. The use of the leaf and flower part in powdered form is acceptable in the amount of 4% max. Within the laboratory production of hemp-enriched cut-out biscuits and bread buns of standard consumer quality, the expected rise in the content of protein and dietary fibre was confirmed.
The effect of the Coefficient Function on the Solution Behavior for the Second-Order Complex Differential Equation
Authors: Alhily Shatha S. , Saleh Haneen Abbas 
Published: May 2022

The purpose of this research paper is to present the second-order homogeneous complex differential equation , where , which is defined on the certain complex domain depends on solution behavior. In order to demonstrate the relationship between the solution of the second-order of the complex differential equation and its coefficient of function, by studying the solution in certain cases: a meromorphic function, a coefficient of function, and if the solution is considered to be a transformation with another complex solution. In addition, the solution has been provided as a power series with some applications.
Stability for the Systems of Ordinary Differential Equations with Caputo Fractional Order Derivatives
Authors: Taher Asad J. , Fadhel Fadhel S. , Hasan Nabaa N. 
Published: May 2022

Fractional calculus has paid much attention in recent years, because it plays an essential role in many fields of science and engineering, where the study of stability theory of fractional differential equations emerges to be very important. In this paper, the stability of fractional order ordinary differential equations will be studied and introduced the backstepping method. The Lyapunov function is easily found by this method. This method also gives a guarantee of stable solutions for the fractional order differential equations. Furthermore it gives asymptotically stable.
Diagnostic Efficacy of Electrical Impedance Spectroscopy Versus Dermoscopy for Pigmented Skin Lesions: A Pilot Study
Authors: Owji Shayan , Han Joseph , He Helen , Lopera Isabel , Tassavor Michael , Brownstone Nicholas , Gulati Nicholas , Ungar Benjamin , Ungar Jonathan 
Published: May 2022

Introduction: Electrical impedance spectroscopy (EIS) is a non-invasive diagnostic device that measures the electrical impedance of skin lesions to assist in the detection of melanoma. While this tool has been shown to have a high sensitivity for melanoma diagnosis, data on its impact on clinical decision-making for pigmented skin lesions (PSLs) compared to other diagnostic tools is lacking. To gain further insight into its clinical utility, we conducted a pilot study to evaluate how this technology – specifically, the effect it has on clinical decisions for PSLs – compares to traditional dermoscopy.
Methods: Dermatologists, dermatology residents, and medical students completed an online survey eliciting their biopsy decisions for 24 PSLs of varying histopathological diagnoses. Half of the lesions from each diagnosis group were presented as a clinical image with associated dermoscopic image and the other half as a clinical image with the corresponding EIS score.
Results: Decisions made with EIS demonstrated a mean sensitivity of 75% for melanomas/severely dysplastic nevi vs. 66% for decisions made with dermoscopy (p=.008). While dermatologists biopsied with similar sensitivities when using EIS or dermoscopy (81% vs. 81%), residents and medical students biopsied with significantly greater sensitivity when using EIS. Respondents who reported rarely using dermoscopy showed the greatest improvement in sensitivity and specificity when using EIS compared to dermoscopy.
Conclusion: Given that not all providers are trained in dermoscopy, and our finding that EIS particularly benefits those who infrequently use dermoscopy, EIS may complement dermoscopy by helping a broader range of providers make improved PSL diagnostic decisions.
Intraparenchymal pericatheter cyst as an indicator of ventriculoperitoneal shunt malfunction: A case-based update
Authors: de Oliveira Samuel Teixeira , Cavalcante-Neto Joaquim Francisco , Matos Luís Eduardo Oliveira , Leal Paulo Roberto Lacerda , Ribeiro Espártaco Moraes Lima , Cristino-Filho Gerardo , da Ponte Keven Ferreira 
Published: May 2022

Background:
Intraparenchymal pericatheter cysts (IPCs) are a rare ventriculoperitoneal shunt (VPS) complication, with only a few cases recorded in the literature.

Case Description:
We report a 22-year-old woman admitted with headache, papilledema, vision loss, and a history of leukemia. Lumbar puncture revealed idiopathic intracranial hypertension (IIH). Three months after VPS implantation, she was readmitted with headache and worsening of visual impairment. CT evidenced a IPC with perilesional edema. Intraoperatively, a shunt revision and cyst drainage were opted for. We present a discussion and literature review on this unique complication of VPS, with emphasis on management.

Conclusion:
It is important to understand and consider IPCs as complications of VPS surgery, including in adult patients and IIH cases.
ECONOMIC – VALUABLE SIGNS AND CHEMICAL COMPOSITION OF THE LEAVES OF NEW MULBERRY VARIETIES IN THE SPRING AND SUMMER SEASON
Authors: Ялгашев Х.А. , Умаров Ш.Р. , Насириллаев Б.У. , Батирова А.Н. 
Published: May 2022

В этой статье изложены анализ хозяйственно-ценных признаков и химического состава листьев новых созданных сортов шелковицы Мархамат, Мустакиллик-2018, Жарарик-12 и контрольного сорта Таджикская бессемянная в весенний и летний сезоны. По каждому из вышеуказанных сортов изучены такие показатели, как площадь листа (длина, ширина, черешок), масса листа (лист с черешком, черешок, масса самого листа) и проценты азота, белка, золы, полипропенолов, β-ситостерина и витамина Е в составе листа, а также дана оценка питательным свойствам сортов шелковицы и рекомендации по внедрению в производство этих сортов в условиях фермерских хозяйств, специализированных к выкормке тутового шелкопряда.
This article presents an analysis of economically valuable traits and the chemical composition of the leaves of new created mulberry varieties Markhamat, Mustakillik-2018, Jararik-12 and the control variety Tajikistan seedless in the spring and summer seasons. For each of the above varieties, such indicators as leaf area (length, width, petiole), leaf weight (leaf with petiole, petiole, weight of the leaf itself) and the percentages of nitrogen, protein, ash, polypropenols, β-sitosterol and vitamin E in composition of the leaf, as well as an assessment of the nutritional properties of mulberry varieties and recommendations for the introduction of these varieties into the production in the conditions of farms specialized in feeding the silkworm.
MORPHOFUNCTIONAL PROCESS OF RECOGNITION AND BLOCKING OF THE ACTIVITY OF CANDIDA ALBICANS BY IMMUNOCOMPETENT CELLS UNDER THE INFLUENCE OF A MICROBIAL ENZYME WITH ADAPTOGENS
Authors: Маннапова Р.Т. , Шайхулов Р.Р. 
Published: May 2022

Кандидамикозы пищеварительного тракта гусей проявлялись в виде активизации и значительного увеличения содержания Candida albicans во всех отделах пищеварительного тракта. Применение энзимного микробного препарата – литиказы, в комплексе с адаптогенами: пробиотиком, прополисом и энзима с пробиотиком и прополисом, на фоне развития кандидамикозов пищеварительного тракта гусей, способствовало восстановлению функционально детерминированных нарушений микробиоценоза на фоне кандидамикозов в целом, что было представлено в наших предыдущих сообщениях, и снижению, в сторону физиологических значений, содержания Candida albicans в зобе в 3,56–11,9, мышечном отделе желудка в 3,52–16,5, железистом – в 10,7–17,8, тонком отделе кишечника в 6,56–20,7, толстом – в 7,08–12,7 раза. При этом развитие защитно – приспособительных и восстановительно морфо-функциональных реакций клеток крови, проявлялись усилением гемо-эритропоэза в 2,6–3,2 раза; повышением процессов распознавания и блокирования активности кандид базофилами в 2,0–2,4 раза; активизацией фагоцитоза кандид псевдоэозинофилами в 2,86–2,93 раза; повышением растворения остатков кандид и снижением действия их токсинов эозинофилами в 2,3–2,52 раза; усилением иммунной защиты лимфоцитами в 6,84–7,15 раза; активизацией продукции моноцитов – как следствие усиления фагоцитоза макрофагов в органах, в 11,0–11,8 раза.
Candidamicosis of the digestive tract of geese manifested itself in the form of activation and a significant increase in the content of Candida albicans in all parts of the digestive tract.The use of an enzyme microbial preparation – lyticase, in combination with adaptogens: a probiotic, propolis and an enzyme with a probiotic and propolis, against the background of the development of candidiasis of the digestive tract of geese, contributed to the restoration of functionally determined disorders of microbiocenosis against the background of candidiasis in general, which was presented in our previous reports, and a decrease, towards physiological values, in the content of Candida albicans in the goiter in 3.56–11.9, in the muscular section of the stomach in 3.52– 6.5, in the glandular – in 10.7–17.8, in the small intestine in 6 .56–20.7, thick – 7.08–12.7 times. At the same time, the development of protective-adaptive and restorative morphofunctional reactions of blood cells was manifested by an increase in hemo-erythropoiesis by 2.6–3.2 times; an increase in the processes of recognition and blocking of Candida activity by basophils by 2.0–2.4 times; activation of Candida phagocytosis by pseudoeosinophils by 2.86–2.93 times; an increase in the dissolution of Candida residues and a decrease in the action of their toxins by eosinophils by 2.3–2.52 times; strengthening of immune protection by lymphocytes by 6.84–7.15 times; activation of monocyte production – as a result of increased phagocytosis of macrophages in organs, by 11.0–11.8 times.
COMPENSATORY REACTIONS OF ORGANELLES OF GENERAL AND SPECIAL PURPOSE IN HEPATOCYTES IN CANDIDIASIS OF THE DIGESTIVE TRACT OF GEESE
Authors: Шайхулов Р.Р. , Маннапова Р.Т. 
Published: May 2022

На фоне кандидамикозов пищеварительного тракта гусей в гепатоцитах печени происходят ультраструктурные компенсаторные реакции органелл общего и специального назначения, направленные на восстановление функций клеток. Так при кандидамикозах пищеварительного тракта в цитоплазме гепатоцитов печени выявляются признаки редукции белоксинтезирующих органелл в связи с изменениями синтетических процессов на полирибосомах и катаболизма в клетках, приводящих к нарушению функции желчеобразования и желчеотделения.Терапия кандидамикозов пищеварительного тракта микробным энзимным препаратом литиказой в комплексе с прополисом и пробиотиком способствует развитию восстановительных морфологических процессов в структурно-функциональных единицах – дольках печени и в гепатоцитах. Ультраструктурная организация гепатоцитов, эндотелиальных клеток синусоидов, клеток Купфера и клеток Ито на фоне проведенной комплексной терапии энзимом литиказой с прополисом и пробиотиком восстанавливается для осуществления функций как желчеобразования, так и желчеотделения.
Against the background of candidiasis of the digestive tract of geese, ultrastructural compensatory reactions of general and special-purpose organelles occur in hepatocytes of the liver, aimed at restoring cell functions. Thus, in candidiasis of the digestive tract in the cytoplasm of hepatocytes of the liver, signs of reduction of protein-synthesizing organelles are revealed due to changes in the synthetic processes on polyribosomes and catabolism in cells, leading to impaired function of bile formation and bile secretion.Therapy of candidiasis of the digestive tract with the microbial enzyme preparation lyticase in combination with propolis and probiotic promotes the development of regenerative morphological processes in structural and functional units – liver lobules and hepatocytes. The ultrastructural organization of hepatocytes, endothelial cells of sinusoids, Kupffer cells and Ito cells against the background of complex therapy with the enzyme lyticase with propolis and a probiotic is restored to perform the functions of both bile formation and bile secretion.
THE GENUS TRIFOLIUM IN THE SOUTH OF THE CENTRAL RUSSIAN UPLAND: ASSESSMENT OF SPECIES DIVERSITY AND SEARCH FOR SOURCE MATERIAL FOR BREEDING
Authors: Думачева Е.В. , Сопина Н.А. , Пономарева А.С. 
Published: May 2022

Изучен видовой состав фитоценозов с участием видов рода Trifolium в условиях стационар балки «Солонцы» (Красногвардейский район, Белгородская область). Виды рода клевер встречаются в степных сообществах луговых и северных степей. Всего на участке стационара идентифицировано 25 семейств, которые представлены 85 родами и 112 видами. Во флористическом списке семейство Fabaceae занимает второе место и представлено 16 видами из 10 родов, в том числе 5 видами клевера. Изучены пределы изменчивости основных морфо-биологические признаков и свойств видов клевера лугового и ползучего (T. pratense L.; T. repens L.). Проведен отбор ценных форм видов клевера лугового и ползучего по признакам высокой семенной продуктивности для дальнейшей селекционной работы. Отобраны особи (12 образцов клевера лугового и 10 образцов клевера ползучего), имеющие высокую степень завязывания семян – не менее 50 % от общего количества цветков в головке, с урожайностью семян для клевера лугового от 12,0 до 15,9 г/ м2 и клевера ползучего – от 9,0 до 11,3 г/ м2.
The species composition of phytocenoses with the participation of species of the genus Trifolium was studied in the conditions of the stationary beam «Solontsy» (Krasnogvardeisky district, Belgorod region). Species of the genus clover are found in steppe communities of meadow and northern steppes. In total, 25 families were identified at the station site, which are represented by 85 genera and 112 species. In the floristic list, the Fabaceae family ranks second and is represented by 16 species from 10 genera, including 5 clover species. The limits of variability of the main morpho-biological features and properties of red and creeping clover species (T. pratense L.; T. repens L.) were studied. The selection of valuable forms of species of red clover and creeping clover was carried out on the basis of high seed productivity for further breeding work. Individuals were selected (12 samples of red clover and 10 samples of red clover) with a high degree of seed setting – at least 50% of the total number of flowers in the head, with a seed yield for red clover from 12.0 to 15.9 g/m2 and clover creeping – from 9.0 to 11.3 g / m2.
INFLUENCE OF INNOVATION AND INVESTMENT ACTIVITIES ON THE DEVELOPMENT OF AZERBAIJAN OIL AND GAS INDUSTRY
Authors: Гасумов Э.Р. , Велиев В.М. , Гасумов Р.А. 
Published: May 2022

В статье рассмотрена роль инвестиции и инновации в развитии нефтегазовой отрасли и определяющей основы экономического роста Азербайджана. Проведен анализ взаимосвязи инноваций и инвестиций как основных факторов в развитии нефтегазовой отрасли, являющейся гарантом обеспечения жизнеспособности и развития государства. Отражено, что взаимодействие инновационного и инвестиционного процесса обеспечивает развитие научно-технического потенциала, государственной экономической безопасности, а главное, это взаимодействие позволяет создать экономические и социальные условия для развития газовой отрасли. Проанализированы факторы, влияющие на реализацию инновационных проектов, темпы развития инновационно-инвестиционной деятельности в нефтегазовой отрасли и ее зависимости от уровня, объемов и структуры инвестирования.
The article considers the role of investment and innovation in the development of the oil and gas industry and the determining basis for the economic growth of Azerbaijan. An analysis was made for the relationship between innovation and investment as the main factors in the development of oil and gas industry which is the guarantor of ensuring the viability and development of the state. It is shown that the interaction of the innovation and investment process ensures the development of scientific and technical potential, state economic security, and most importantly, this interaction allows you to create economic and social conditions for gas industry development. The factors influencing the innovative projects, rate of development of innovative and innovative activities in the oil and gas industry and its dependence on the level, volume and structure of investment are analyzed.