bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ischemic stroke causes Parkinson’s disease-like pathology and symptoms in transgenic mice overexpressing alpha-synuclein
Authors: Lohmann Stephanie , Grigoletto Jessica , Bernis Maria Eugenia , Pesch Verena , Ma Liang , Reithofer Sara , Tamgüney Gültekin 
Published: February 2022

AbstractThe etiology of Parkinson’s disease is poorly understood and is most commonly associated with advancing age, genetic predisposition, or environmental toxins. Epidemiological findings suggest that patients have a higher risk of developing Parkinson’s disease after ischemic stroke, but this potential causality lacks mechanistic evidence. We investigated the long-term effects of ischemic stroke on pathogenesis in hemizygous TgM83 mice, which express human α-synuclein with the familial A53T mutation without developing any neuropathology or signs of neurologic disease for more than 600 days. We induced transient focal ischemia by middle cerebral artery occlusion in 2-month-old TgM83+/− mice and monitored their behavior and health status for up to 360 days post surgery. Groups of mice were sacrificed at 14, 30, 90, 180, and 360 days after surgery for neuropathological analysis of their brains. Motor deficits first appeared 6 months after focal ischemia and worsened until 12 months afterward. Immunohistochemical analysis revealed ischemia-induced neuronal loss in the infarct region and astrogliosis and microgliosis indicative of an inflammatory response, which was most pronounced at 14 days post surgery. Infarct volume and inflammation gradually decreased in size and severity until 180 days post surgery. Surprisingly, neuronal loss and inflammation were increased again by 360 days post surgery. These changes were accompanied by a continuous increase in α-synuclein aggregation, its neuronal deposition, and a late loss of dopaminergic neurons in the substantia nigra, which we detected at 360 days post surgery. Control animals that underwent sham surgery without middle cerebral artery occlusion showed no signs of disease or neuropathology. Our results establish a mechanistic link between ischemic stroke and Parkinson’s disease and provide an animal model for studying possible interventions.
Mutational spectrum of syndromic genes in sporadic brain arteriovenous malformation
Authors: Wang Kun , Zhang Mingqi , Zhao Sen , Xie Zhixin , Zhang Yisen , Liu Jian , Zhang Ying , Yang Xinjian , Wu Nan 
Published: February 2022

Abstract
Background
Brain arteriovenous malformations (BAVMs) are abnormal vessels that are apt to rupture, causing life-threatening intracranial hemorrhage (ICH). The estimated prevalence of BAVMs is 0.05% among otherwise healthy individuals. In this study, we aim to investigate the mutational spectrum of syndromic genes in sporadic BAVM.

Methods
We recruited a cohort of 150 patients with BAVM and performed whole-exome sequencing on their peripheral blood DNA. To explore the mutational spectrum of syndromic genes in sporadic brain arteriovenous malformation, we selected six genes according to the Online Mendelian Inheritance in Man (OMIM) and literature. All variants in the six candidate genes were extracted and underwent filtering for qualifying variants.

Results
There are a total of four patients with rare variants in hereditary hemorrhagic telangiectasia-related genes. In addition, we identified two patients have the variant of RASA1 gene in our database, which are also rare mutations that are absent from population databases. However, we did not find any patients with GNAQ mutations in our database.

Conclusions
In conclusion, we demonstrated that variants in syndromic vascular malformations play important roles in the etiology of sporadic BAVM.
Validity of 1% Hormonal Receptor Positivity Cutoff by the ASCO/College of American Pathologists Guidelines at the Georgia Cancer Center
Authors: Kreidieh Firas , Sadek Ramses F. , Zhang Li Fang , Gopal Aaron , Blaize Jean-Pierre , Yashar David , Patel Reena , Patel Hiral S. , Tang Shou-Ching , Abdul Sater Houssein 
Published: February 2022

PURPOSE Treatment of breast cancer (BC) with borderline or low (1%-9%) estrogen and progesterone expression remains controversial, with recent data disputing ASCO/College of American Pathologists 2010 guidelines that lowered the threshold of receptor positivity from 10% to 1%. The objective of this retrospective study was to validate these guidelines at the Georgia Cancer Center with a high percentage of Black race. METHODS All female patients with invasive BC diagnosed between 2005 and 2010 at the Georgia Cancer Center were chart reviewed up to an 11-year follow-up with data cutoff at 2016. We used Cox regression to explore survival among three hormonal status (HS) groups (< 1%, 1%-9%, and ≥ 10%) adjusting for all known BC clinicopathologic variables. Fisher's exact test was used to evaluate response to endocrine therapy (ET). RESULTS Among 431 patients with mean age 59 years, 24.75% had HS < 1%, 17.5% HS 1%-9%, and 57.75% HS ≥ 10%. Race was 43.75% Black and 54% White. Disease stages were early (I-IIIA) in 84.4% and advanced (IIIB-IV) in 15.56%. Mortality in HS < 1% was significantly higher than that in HS ≥ 10% (hazard ratio [HR]: 1.8; 95% CI, 1.07 to 3.02), whereas no significant mortality difference between HS 1%-9% and HS ≥ 10% (HR: 1.05; 95% CI, 0.48 to 2.30) was observed. ET was protective, and treated patients had higher predicted survival than untreated patients in the 1%-9% group (HR: 0.10; 95% CI, 0.01 to 0.85). There was no significant mortality difference between ET-treated HS 1%-9% and ≥ 10% groups. CONCLUSION One percent cutoff predicted superior survival on treatment with ET compared with the other groups, and HS as low as 1%-9% was equiprognostic to HS ≥ 10%. Whether other factors such as lymphovascular invasion, grade, and other parameters change the behavior of the 1%-9% HS group remains to be explored.
Facilitated Health Coaching Improves Activity Level and Chronic Low back Pain Symptoms
Authors: Ellingson Laura D. , Lansing Jeni E. , Perez Maria L. , DeShaw Kathryn J. , Meyer Jacob D. , Welk Gregory J. 
Published: February 2022

ABSTRACT

Introduction
Behavioral treatments, like increasing physical activity (PA), are recommended for chronic low back pain (CLBP). Finding methods for promoting behavior change with potential for translatability as well as effective behavioral targets remains challenging.

Purpose
This randomized controlled pilot study evaluated the effectiveness of low-dose facilitated health coaching with activity monitors to improve PA and sedentary behaviors (SB), symptoms, and Patient Global Impression of Change (PGIC) in CLBP. A secondary purpose was identifying behaviors associated with symptom change to inform future trials.

Methods
Seventy-one adults with CLBP were randomized to receive a wearable activity monitor alone (WAM) or with one primary session of health coaching and two check-ins based on motivational interviewing (WAM + HC) or a wait-list control (WLC) condition for 12 wk. Moderate and vigorous PA (MVPA), light PA (LPA), total and prolonged SB (i.e., sedentary >60 min per bout), and pain (Short Form McGill Pain Questionnaire (MPQ)) were assessed before and after intervention along with PGIC. Regression analyses examined group differences in MVPA, LPA, total and prolonged SB, MPQ, and PGIC after intervention as well as behavioral predictors of symptom improvement (change in MPQ).

Results
WAM + HC improved MPQ scores (β = −0.25, P = 0.02), LPA (β = 0.23, P = 0.04), MVPA (β = 0.21, P = 0.03), and total SB (β = −0.24, P = 0.03) compared with WLC over time. Both WAM + HC and WAM had significantly higher PGIC compared with WLC (P < 0.05). Change in prolonged SB was the only significant predictor of change in MPQ (β = 0.48, P = 0.01).

Conclusions
WAM + HC may be effective for changing activity-related behaviors and improving CLBP. Furthermore, reducing prolonged SB may be a meaningful target for future interventions in CLBP. Research focused on reducing prolonged SB including larger samples, and examining changes in symptoms will be important for identifying optimal translational treatment strategies for CLBP.
The Neuroimaging Data Model Linear Regression Tool (nidm_linreg): PyNIDM Project
Authors: Kumar Ashmita , Crowley Albert , Queder Nazek , Poline JB , Ghosh Satrajit S. , Kennedy David , Grethe Jeffrey S. , Helmer Karl G. , Keator David B. 
Published: February 2022

The Neuroimaging Data Model (NIDM) is a series of specifications for describing all aspects of the neuroimaging data lifecycle from raw data to analyses and provenance. NIDM uses community-driven terminologies along with unambiguous data dictionaries within a Resource Description Framework (RDF) document to describe data and metadata for integration and query. Data from different studies, using locally defined variable names, can be retrieved by linking them to higher-order concepts from established ontologies and terminologies. Through these capabilities, NIDM documents are expected to improve reproducibility and facilitate data discovery and reuse. PyNIDM is a Python toolbox supporting the creation, manipulation, and querying of NIDM documents. Using the query tools available in PyNIDM, users are able interrogate datasets to find studies that have collected variables measuring similar phenotypic properties. This, in turn, facilitates the transformation and combination of data across multiple studies. The focus of this manuscript is the linear regression tool which is a part of the PyNIDM toolbox and works directly on NIDM documents. It provides a high-level statistical analysis that aids researchers in gaining more insight into the data that they are considering combining across studies. This saves researchers valuable time and effort while showing potential relationships between variables. The linear regression tool operates through a command-line interface integrated with the other tools (pynidm linear-regression) and provides the user with the opportunity to specify variables of interest using the rich query techniques available for NIDM documents and then conduct a linear regression with optional contrast and regularization.
The impact of clinical pharmacist-led health education on the disease course of non-alcoholic fatty liver disease patients: an interventional study
Authors: Abou Seada Nehal , El Hamamsy Manal , Shaheen Sarah , Elwakil Reda , Barakat Alaa , El‐Sayed Mansy Azza 
Published: February 2022

Background: A multitude of health care professionals, including pharmacists, are needed in managing a complex and silent disease like non-alcoholic fatty liver disease (NAFLD) associated with metabolic syndrome (MetS). These health care professionals can increase patients’ health awareness regarding the prevention and the management of the disease. The current study aimed to evaluate the impact of clinical pharmacist education, counseling and follow up on the management of NAFLD with MetS. Methods: This study recruited 102 patients with NAFLD and MetS. Participants received regular health education sessions concerning the disease and recommended lifestyle (diet and exercise) and were followed by clinical pharmacist for 6 months. At the end of the study, participants were divided into two groups; those compliant with education and follow up session (compliant group; n=61); and those attending > 60% but not completing whole sessions (non-compliant group; n=41). Anthropometric measures, liver function, lipid profile, homeostasis model of assessment-insulin resistance (HOMA-IR), biochemical NAFLD score, radiological liver steatosis and fibrosis grade changes were recorded at baseline and endpoint. Results: The compliant group showed a more significant decrease in weight (p=0.003), low density lipoprotein (LDL) (p=0.009), and very LDL (p<0.001), and a more significant increase in high density lipoprotein (HDL) (p=0.010) compared with the non-compliant group. Moreover, the compliant group showed a statistically significant higher number of patients achieving normalization of total cholesterol (p=0.002), HDL (p=0.004), waist circumference (p=0.004), improvement of body mass index category (p=0.008), liver steatosis grade (p=0.009), liver fibrosis grade (p<0.001), and absence of dyspeptic symptoms (p=0.0010) and hepatomegaly (p= 0.027) compared with the non-compliant group. Fasting blood glucose (p=0.209), fasting insulin (p=0.179), and HOMA-IR score (p=0.193) showed non-significant difference between both groups at endpoint. Conclusion: The educational intervention of a clinical pharmacist showed significant positive impact on ameliorating metabolic syndrome parameters and achieving desired NAFLD therapeutic outcomes.
Digital health interventions for gestational diabetes mellitus: A systematic review and meta-analysis of randomised controlled trials
Authors: Leblalta Boutheina , Kebaili Hanane , Sim Ruth , Lee Shaun Wen Huey 
Published: February 2022

Good blood glucose control is important to reduce the risk of adverse effects on mothers and their offspring in women with gestational diabetes (GDM). This review examined the impact of using digital health interventions on reported glycaemic control among pregnant women with GDM and its impact on maternal and foetal outcomes. Seven databases were searched from database inception to October 31st, 2021 for randomised controlled trials that examined digital health interventions to provide services remotely for women with GDM. Two authors independently screened and assessed the studies for eligibility for inclusion. Risk of bias was independently assessed using the Cochrane Collaboration’s tool. Studies were pooled using random effects model and presented as risk ratio or mean difference with 95% confidence intervals. Quality of evidence was assessed using GRADE framework. Twenty-eight randomised controlled trials that examined digital health interventions in 3,228 pregnant women with GDM were included. Moderate certainty of evidence showed that digital health interventions improved glycaemic control among pregnant women, with lower fasting plasma glucose (mean difference -0.33 mmol/L; 95% CI: -0.59 to -0.07), 2-hour post-prandial glucose (-0.49 mmol/L; -0.83 to -0.15) and HbA1c (-0.36%; -0.65 to -0.07). Among those randomised to digital health interventions, there was a lower need for caesarean delivery (Relative risk: 0.81; 0.69 to 0.95; high certainty) and foetal macrosomia (0.67; 0.48 to 0.95; high certainty). Other maternal and foetal outcomes were not significantly different between both groups. Moderate to high certainty evidence support the use of digital health interventions, as these appear to improve glycaemic control and reduce the need for caesarean delivery. However, more robust evidence is needed before it can be offered as a choice to supplement or replace clinic follow up.
Systematic review registration: PROSPERO: CRD42016043009.
Controversias en la elección de curvas de crecimiento para evaluar los recién nacidos colombianos.
Authors: Montealegre Pomar Adriana del Pilar 
Published: February 2022

Las curvas de crecimiento permiten comparar el patrón de crecimiento de cada niño(a), con aquel que se considera apropiado para su grupo etario. En los recién nacidos, una correcta clasificación del crecimiento ponderal hace posible tratar y prevenir oportunamente la morbimortalidad infantil asociada a desviaciones en el crecimiento. Actualmente existen múltiples tipos de curvas (estandarizadas, de referencia local o internacional, personalizadas) y no se tiene certeza sobre cuál de ellas evalúa mejor el crecimiento de los recién nacidos. La evidencia indica que se debe conocer el patrón de crecimiento de cada país o región antes de adoptar cualquier tipo de curva. En Colombia se tienen pocos estudios sobre curvas locales y se han adoptado curvas internacionales sin haberlas validado para nuestra población. Es fundamental generar curvas propias y conocer los pasos necesarios para adoptar una curva internacional, porque: 1) Las curvas internacionales pueden llevar a errores de clasificación, debido a problemas de representatividad. 2) Como consecuencia, se necesita conocer el patrón de crecimiento local para saber si la curva internacional se ajusta a nuestra población, y finalmente, 3) Si se desea adoptar una curva de crecimiento, es indispensable evaluar la metodología empleada para su creación, comparar los patrones de crecimiento de la curva seleccionada y la curva local y conocer el contexto clínico en el que se aplicará la curva. Estos aspectos, permitirán elegir un instrumento adecuado con el que se puedan tomar medidas de prevención o terapéuticas acertadas.
Left Ventricular Assist Device Support and Longitudinal Sleeve Gastrectomy Combined With Diet in Bridge to Heart Transplant
Authors: Ng Mandy , Rodgers Beverly , Rehman Saadiya , Nathan Sriram S. , Bajwa Kulvinder S. , Shah Shinil K. , Akkanti Bindu H. , Jumean Marwan F. , Kumar Sachin , Dressel Jennifer L. , Radovancevic Rajko , Felinski Melissa M. , Kar Biswajit , Gregoric Igor D. 
Published: February 2022

Combining left ventricular assist device (LVAD) implantation and longitudinal sleeve gastrectomy may enable patients with morbid obesity to lose enough weight for heart transplant eligibility. In a retrospective study, we evaluated long-term outcomes of patients with body mass indexes ≥35 who underwent LVAD implantation and longitudinal sleeve gastrectomy during the same hospitalization (from January 2013 through July 2018) and then adhered to a dietary protocol. We included 22 patients (mean age, 49.9 ± 12.5 yr; mean preoperative body mass index, 43.3 ± 6.2). Eighteen months after gastrectomy, all 22 patients were alive, and 16 (73%) achieved a body mass index of less than 35. Myocardial recovery in 2 patients enabled LVAD removal. As of October 2020, 10 patients (45.5%) had undergone heart transplantation, 5 (22.3%) were waitlisted, 5 (22.3%) still had a body mass index ≥35, and 2 (9%) had died.
With LVAD support, longitudinal sleeve gastrectomy, and dietary protocols, most of our patients with morbid obesity and advanced heart failure lost enough weight for transplant eligibility. Support from physicians and dietitians can maximize positive results in these patients.
Development and Performance Evaluation of Automated Methadone Dispenser for Drug Addiction Therapy
Authors: Mohd Mazlan Muhamad Farhan , Salleh Siti Zuliana , Ab Karim Mohd Sayuti , Mohd Amran Nor Amirah , Abd Rashid Rusdi , Abd Razak Nasrul Anuar , Kadri Nahrizul Adib , Zahari Zalina 
Published: February 2022

Abstract
Drug abuse and drug addiction are becoming growing issues in most countries worldwide and are considered major public health problems. An initiative has been introduced in Malaysia via the methadone maintenance treatment program since the year 2005. Although the program has shown gradual success over the years by reducing the relapse rate of the patients, it has encountered several challenges in maintaining the effectiveness of the manual dispensing method done by the authorized health practitioners. Therefore, an automated methadone dispenser was developed to improve the effectiveness and comply with the requirements and needs of the users. In this research, the reliability and feasibility of the dispenser were determined through percentage error, accuracy and efficiency, and tests, respectively. Based on the performance evaluation, the findings suggested that the automated methadone dispenser has contributed to the decrease of percentage error to 3.42 %, 31.52 % higher accuracy, and 96 % better efficiency compared to current manual methadone dispensing practices.
OCCURRENCES OF TROPICAL ANGUILLIDS EELS IDENTIFIED THROUGH DNA BARCODES AT KEDURANG RIVER, SUMATRA ISLAND, INDONESIA
Authors: Wibowo Arif , Antony Rezki , Samuel Samuel , Musch Anna-Lena , Atminarso Dwi 
Published: February 2022

Understanding the geographic distribution of tropical anguillids is important for the conservation of these species. Delimitation of species distribution area is a fundamental task with important implications for the understanding of biodiversity and conservation. However, their geographic ranges are poorly understood, mostly because of the technical difficulties of identifying anguillids by morphological characteristics, especially at the glass eel stage. The current work aims to provide the information on recruitment of tropical anguilid eels in the Kedurang River by using analysis of species identification of an informative DNA segment of Cytochrome Oxidase Sub unit1. Results showed that PCR, sequencing and analysis of an informative DNA can be a useful complement to morphological study for more complete biodiversity assessments. The anguillid eels found in Kedurang River were identiﬁed and further validated as A. bicolor bicolor, A. interioris, A. bengalensis bengalensis, and A. marmorata through glass and adult eels. This study suggests the occurrence of A. interioris in Estuary of Sumatera River would provide the first confirmation for this species in the territory. The information can be useful for understanding the geographic distribution this species for the establishment and allocation of risk categories to species, both in national protection lists and in those of treaties and international conventions.
Sebaran Ukuran Rajungan Hasil Tangkapan Gillnet dengan Jarak Lokasi Penangkapan Berbeda di Perairan Kabupaten Pangkep
Authors: Ihsan Ihsan , Tajuddin Mustamin , Abdullah Hasrun , Zainuddin Zainuddin 
Published: February 2022

Penelitian bertujuan menkaji frekwensi sebaran ukuran dan jumlah hasil tangkapan gillnet jarak lokasi penangkapan berbeda. Data dibutuhkan adalah data primer dan sekunder. Bahan dan alat digunakani alat mistar panjang, kamera, dan rajungan. Data dianalisis menggunakan analisis distribusi frekwensi ukuran hasil tangkapan rajungan. Struktur frekwensi sebaran ukuran (cm) produksi rajungan dengan alat gillnet terhadap lokasi penangkapan berbeda di perairan Pulau Balang Lompo Kabupaten Pangkep menunjukan adanya perbedaan, umumnya ukuran paling kecil jarak 0-2 mil, dan ukuran jarak >2-4 mil dan jarak >4-6 mil, produks rajungan semakin besar. Perbedaan nyata dari segi perbedaan ukuran produksi rajungan, lebih kecil dekat pantai dan jumlah lebih banyak dibandingkan lepas pantai. Jarak lebih jauh dari pantai produksi rajungan, sebaran ukuran produksi rajungan besar dan jumlahnya banyak dari perairan lebih dekat Pulau Balang Lompo Kabupaten Pangkep.
Kesesuaian Lahan Budidaya Lobster (Panulirus spp.) Sistem Keramba Jaring Apung Menggunakan Pendekatan Sistem Informasi Geografis
Authors: Prasetya Arif , Hasidu La Ode Abdul Fajar 
Published: February 2022

Penelitian ini bertujuan mengidentifikasi dan memetakan beberapa parameter oseanografi yang sesuai untuk kegiatan budidaya lobster sistem keramba jaring apung dan menghasilkan rekomendasi berupa peta spasial kelayakan lahan budidaya lobster sistem keramba jaring apung. Penelitian ini dilaksanakan pada bulan Juli 2021 di perairan Kabupaten Kolaka. Pengambilan data oseanografi dilakukan pada 58 stasiun, selanjutnya melakukan spasial analisis menggunakan program Arc GIS 10. Metode menggunakan analisis interpolasi dan klasifikasi spasial, kemudian melakukan overlay data pada seluruh parameter oseanografi untuk memberikan skoring berdasarkan matriks kesesuaian, sehingga menghasilkan peta spasial kesesuaian lahan. Berdasarkan peta kesesuaian lahan, diperoleh luas lahan yang sangat sesuai untuk budidaya lobster sistem keramba jaring apung adalah 7973 ha dan cukup sesuai seluas 20090 ha, sedangkan seluas 9519 tidak sesuai untuk kegiatan budidaya lobster sistem keramba jaring apung di perairan Kabupaten Kolaka, Provinsi Sulawesi Tenggara.