bluuebunny/crossref_metadata_2025_split huggingface dataset:
LILRB2/PirB mediates macrophage recruitment in fibrogenesis of nonalcoholic steatohepatitis
Authors: Chen Yong , yu xue , Li Danpei , Huang Li , Meng Xiao-Yu , Wang Shu-Yun , Kan Ran-Ran , Zou Hua-Jie , Guo Ya-Ming , Pan Li-Meng , Luo Pei-Qiong , Xiang Yu-Xi , Mao Bei-Bei , Wang Zhi-Han , He Rui , Yang Yan , Liu Zhelong , Xie Jun-Hui , Ma Delin , Zhang Ben-Ping , Shao Shi-Ying , Chen Xi , Xu Simiao , He Wen-Tao , Li Wen-Jun 
Published: September 2022

Abstract
Inhibition of immunocyte infiltration and activation has been proven to effectively ameliorate hepatic inflammation and fibrosis in nonalcoholic steatohepatitis (NASH). Paired immunoglobulin-like receptor B (PirB) and its human orthologue receptor, leukocyte immunoglobulin-like receptor B (LILRB2), are immune-inhibitory receptors with unknown roles in NASH. Here, we demonstrate that PirB/LILRB2 regulates the migration of macrophages in NASH pathogenesis and fibrogenesis by binding to its NASH-associated ligand angiopoietin-like protein 8 (ANGPTL8). Mechanistically, PirB facilitates the ANGPTL8-induced infiltration of monocyte-derived macrophages (MDMs) into the liver by regulating the phosphorylation of P38, AKT, and P65. Hepatocyte-specific knockout of its ligand ANGPTL8 reduces MDM infiltration and resolves lipid accumulation and fibrosis progression in the livers of NASH mice. In addition, PirB−/− bone marrow (BM) chimaeras abrogated ANGPTL8-induced MDM migration to the liver. PirB ectodomain protein can ameliorate the lipid accumulation inflammatory response and fibrosis of NASH by sequestering ANGPTL8. Furthermore, LILRB2-ANGPTL8-axis-associated MDM migration and inflammatory activation are also observed in human peripheral blood monocytes. Taken together, our findings reveal a novel role of PirB/LILRB2 in NASH pathogenesis and identify PirB/LILRB2-ANGPTL8 signalling as a potential target for the management or treatment of NASH.
Convolutional neural network for detecting rib fractures on chest radiographs: A feasibility study
Authors: Wu Jiangfen , Liu Nijun , Li Xianjun , Shang Jin , Wang Fei , Chen Bowei , Shen Yuanwang , Cao Pan , Liu Zhe , Li Miaoling , Fan Qianrui , Qian Jiayao , Li Zhihao , Yang Jian , Sun Qinli 
Published: September 2022

Abstract
Background: The application of artificial intelligence for the detection of rib fractures on chest radiographs is limited by image quality control and multi-lesion screening. We aimed to create a model for multiple rib fracture detection using a convolutional neural network (CNN) based on quality-normalised chest radiographs.Methods: A total of 1,080 radiographs with rib fractures were obtained and randomly divided into training (918 graphs, 85%) and testing (162 graphs, 15%) sets. An object detection CNN, you only look once (YOLO) v3, was adopted to build the detection model. Receiver operating characteristic (ROC) and free-response ROC (FROC) were used to evaluate model performance. A joint testing group of 162 radiographs with rib fractures and 233 radiographs without rib fractures was used as the internal testing set. Furthermore, additional 201 radiographs, 121 with rib fractures and 80 without rib fractures, were independently validated to compare the CNN model performance with the diagnostic efficiency of radiologists.Results: The sensitivity of the model in the training and testing sets was 92.0% and 91.1%, respectively, and the precision was 68.0% and 81.6%, respectively. FROC in the testing set showed that the sensitivity for whole-lesion detection reached 91.3% when the false-positive of each case was 0.56. In the joint testing group, the case-level accuracy, sensitivity, specificity, and area under the curve were 85.1%, 93.2%, 79.4%, and 0.92, respectively. In the independent validation set, at the fracture level, the sensitivity of the CNN model (87.3%) was higher than that of the senior (80.3%) and junior radiologists (73.4%), while the precision (80.3%) was slightly lower than that of the latter two (82.4% and 81.7%, respectively). At the case level, the accuracy and sensitivity of the CNN model (91.5% and 96.7%, respectively) were both higher than those of the junior radiologist (85.1% and 77.7%, respectively) and close to those of the senior radiologist (94.0% and 96.7%, respectively). Conclusions: The CNN model based on YOLOv3 is sensitive for detecting rib fractures on chest radiographs and shows great potential in the preliminary screening of rib fractures.
Joint Learning Sample Similarity and Correlation Representation for Cancer Survival prediction
Authors: Hao Yaru , Jing Xiaoyuan , Sun Qixing 
Published: September 2022

Abstract
Background: As a highly aggressive disease, cancer has been becoming the leading death cause around the world. Accurate prediction of the survival expectancy for cancer patients is significant, which can help clinicians make appropriate therapeutic schemes. With the high-throughput sequencing technology becoming more and more cost-effective, integrating multi-type genome-wide data has been a promising method in cancer survival prediction. Based on these genomic data, some data-integration methods for cancer survival prediction have been proposed. However, existing methods fail to simultaneously utilize feature information and structure information of multi-type genome-wide data. Results: We propose a Multi-type Data Joint Learning (MDJL) approach based on multi-type genome-wide data, which comprehensively exploits feature information and structure information. Specifically, MDJL exploits correlation representations between any two data types by cross-correlation calculation for learning discriminant features. Moreover, based on the learned multiple correlation representations, MDJL constructs sample similarity matrices for capturing global and local structures across different data types. With the learned discriminant representation matrix and fused similarity matrix, MDJL constructs graph convolutional network with Cox loss for survival prediction. Conclusions: Experimental results demonstrate that our approach substantially outperforms established integrative methods and is effective for cancer survival prediction.
On a General Class of Geometric Constants and Normal Structure in Banach Spaces
Authors: Dinarvand Mina 
Published: September 2022

Abstract

In this paper, we establish the lower bounds for the weakly convergent sequence coefficient WCS(X) of a Banach space X, in terms of the family of geometric constants Cλ,p(X), the coefficient of weak orthogonality µ(X) and the generalized García-Falset coefficient R(1, X). By mean of these bounds, we identify some geometrical properties implying normal structure. Moreover, some sufficient conditions which imply uniform normal structure are presented. Our results significantly generalize and improve many known results in the literature.Mathematics Subject Classification (2020): Primary 46B20; Secondary 47H09.
Fibroblast-selective smoothened governs the prognosis of acute kidney injury
Authors: Gui Yuan , Fu Haiyan , Palanza Zachary , Tao Jianling , Lin Yi-Han , Min Wenjian , Yi Qiao , Bonin Christopher , Hargis Geneva , Wang yuanyuan , Kreutzer Donald , Wang Yanlin , Liu Yansheng , Yu Yanbao , Liu Youhua , Zhou Dong 
Published: September 2022

Abstract
The smoothened (Smo) receptor facilitates hedgehog signaling between kidney fibroblasts and tubules during acute kidney injury (AKI). Tubule-derived hedgehog is protective in AKI, but the role of fibroblast-derived Smo is unclear. Here, we report that Smo ablation in fibroblasts mitigated tubular cell apoptosis and inflammation, enhanced perivascular mesenchymal activities, and preserved kidney function after AKI. Global proteomics of these kidneys identified extracellular matrix proteins, and nidogen-1 glycoprotein in particular, as key response markers; Intriguingly, Smo was bound to nidogen-1 in cells, suggesting that loss of Smo could impact nidogen-1 accessibility. Phosphoproteomics revealed that the ‘AKI protector’ Wnt pathway was activated in these kidneys, and in vitro and ex vivo, nidogen-1 was able to induce Wnts and repress tubular cell apoptosis. Altogether, our results support that fibroblast-derived Smo dictates AKI fate through cell-matrix interactions, including nidogen-1, and establish a robust resource and path to further dissect AKI pathogenesis.
Robust performance of the methylated NTMT1 and MAP3K14-AS1 dual-target test for colorectal cancer detection in plasma by using sense-antisense and dual-MGB probe technique
Authors: Zhao Yanteng , Wang Zhijie , Yu Qiuning , Liu Xin , Liu Xue , Dong Shuling , Lv Xianping , Bai Yu , Wang Shaochi 
Published: September 2022

Abstract
Background
Blood-based methylation tests showed higher patient compliance and convenience than colonoscopy and stool DNA tests for colorectal cancer (CRC) detection. This study aimed to enhance the performance of plasma markers to detect CRCs using sense-antisense and dual-MGB probe (SADMP) technique.
Method
The current study consisted of 3 phases. The first phase was identifying eligible methylation markers. The second phase was assay development that incorporated the SADMP technique into methylation-specific PCR to enhance the target sensitivities in detecting plasma methylation signals. The third phase was assessing the test performance for CRC detection in training and validation cohorts.
Results
Hypermethylated NTMT1 and MAP3K14-AS1 were found in multiple CRC cohorts. The SADMP technique showed an approximately 2-fold increase in detecting methylation signals than single-strand and single-MGB probe techniques. The established MethyDT test obtained an average sensitivity of 84.47% for CRC detection, higher than any single target alone without a significant attenuation of specificity (average specificities of 91.81% for NTMT1 and 96.93% for MAP3K14-AS1 vs. 89.76% for MethyDT). For early (I-II) and late- (III-IV) stage CRC, the test sensitivities were 82.61% and 88.64%, respectively, in validation cohort. Meanwhile, the test sensitivity was independent of patient age and gender.
Conclusion
The SADMP technique enhanced the detection of methylation signals, and the MethyDT test showed potential utility as a promising noninvasive tool for CRC detection.
Existence and stability analysis for a general non-linear singular delay fractional differential equation with p-Laplacian operator
Authors: Kaushik Kirti Kaushik Kirti , Kumar Anoop Kumar Anoop 
Published: September 2022

Abstract
In this manuscript, the major objective is to investigate the existence and uniqueness (EU) of positive solutions for a general class of nonlinear fractional differential equation (FDE) with fractional differential and integral boundary conditions involving φp-Laplacian operator. To continue, we’ll utilize Green’s function to get the suggested FDE’s corresponding integral form. The existence findings are achieved by combining the Guo-Krasnosel’skii fixed point theorem with the features of the p-Laplacian operator. Additionally, Hyers-Ulam (HU) stability is also examined. Further, an example is presented to illustrate the effectiveness.
Lethal and sublethal effects of commonly used insecticides on South American tomato pinworm, Tuta absoluta Meyrick and its parasitoid, Trichogramma brassicae Bezdenko
Authors: Papari Salma , Dousti AbuFazel , Fallahzadeh Majid , Ullah Farman , Desneux Nicolas , Saghaei Nazila 
Published: September 2022

Abstract
The South American tomato pinworm, Tuta absoluta Meyrick, which is native to South America, emerged as a major invasive insect pest of tomato plants worldwide. Tuta absoluta can cause up to near 100% crop loss if not effectively controlled. Initially, insecticides were used to control the larvae, but this pest has developed resistance to commonly used insecticide-active substances. Therefore, new biological and chemical control options have been adopted to limit severe crop damage caused by T. absoluta larvae. In this study, the lethal and sublethal effects of four insecticides (flubendiamide, emamectin benzoate+lufenuron, thiocyclam, and spinosad) were investigated on T. absoluta, and its parasitoid, Trichogramma brassicae Bezdenko under controlled laboratory conditions. The results showed that spinosad with an LC50 value of 7.05 mg a.i/L was highly toxic against third instar larvae of T. absoluta in comparison to emamectin benzoate+lufenuron (14.24 mg a.i/l), flubendiamide (37.29 mg a.i/l) and thiocyclam (65.17 mg a.i/l). Spinosad with an LC50 of 0.73 mg a.i/l was also have higher toxicity against adult parasitoids compared to flubendiamide (4.09 mg a.i/l), emamectin benzoate+lufenuron (4.17 mg a.i/l) and thiocyclam (9.59 mg a.i/l). Furthermore, key demographic parameters such as ross net reproductive rate (R0), intrinsic rate of increase (rm), finite rate of increase (λ), mean generation time (T) and doubling time (DT) were significantly decreased after exposure to the LC30 of spinosad followed by thiocyclam, flubendiamide, and emamectin benzoate+lufenuron. Similar trends were observed for the population parameters (R0, rm, λ, T, and DT) of T. brassicae flowing exposure to the LC30 of spinosad, thiocyclam, flubendiamide, and emamectin benzoate+lufenuron. Taken together, our results showed that thiocyclam and flubendiamide insecticides are highly toxic against T. absoluta while having low toxicity against their parasitoid, T. brassicae. These findings may be valuable in controlling T. absoluta using flubendiamide and thiocyclam along with T. brassicae in integrated management programs.
Synthesized Nano composites of nano silica and reduced graphene with urea for nitrogen fertilizer capsule production and their evaluation
Authors: Ram Shri , sadiq Mubasir Sadiq ,   Subhaum Anil Durgude3 durgude, Singh Virendra Singh 
Published: September 2022

Abstract

Finding the replacement of urea for nitrogen fertilization, mesoporous nano silica (mNs) and reduced graphene oxide (rGO)-based urea nanocomposites were synthesized. These composites were characterized by UV-spectroscopy, FTIR, XRD, FE-SEM and HR-TEM to examine surface functional groups, morphology, and structural composition. XRD spectrum confirmation with SAED image of nano silica and graphene oxide nanocomposites confirms the polycrystalline and crystalline nature with 45–70 nm crystal size. The SEM revealed that the modified surface of mesoporous nano silica and reduced graphene oxide are well-distributed clusters and are composed of targeted nutrient. Synthesized composites were evaluated on seed germination, crop productivity and N use efficiency (NUE). The grain yields, NPK uptake and nitrogen use efficiency in rice and wheat was significantly higher with mNs and rGO-based composites applied to supply @ 60 N Kg ha− 1 as well as led to nutrients availability and microbial properties by increasing NPK content in soil over conventional use of urea. Application of mNs for 120 kg N ha− 1 showed maximum 64.44% increase in rice and 59% in wheat grain yields over conventional fertilization (120 kg ha− 1 through urea). Besides these, significant increment in grain yields, NPK content and uptake by crops was recorded Over control. Maximum 75.71 and 91.36% increase in NUE by rice and wheat crops, respectively by rice and wheat, respectively compared with conventional fertilization (120 kg ha− 1 through ordinary urea)
The remaining parameters of patellar instability could be affected for osteoarthritic change after medial patellofemoral ligament reconstruction with or without anteromedialization of the tibial tubercle osteotomy for patellar instability: A retrospective cohort study
Authors: Hashimoto Yusuke , Nishino Kazuya , Tomihara Tomohiro , Tsumoto Shuko , Nakamura Hiroaki 
Published: September 2022

Abstract
Background
In literature, studies evaluating the factors associated the postoperative progression of patellofemoral (PF) osteoarthritis (OA) following patellar stabilization surgery are limited. This study aimed to compare the clinical outcomes after medial patellofemoral ligament reconstruction (MPFLR) as an isolated procedure (iMPFLR) and in combination with anteromedialization (AMZ) of the tibial tubercle osteotomy (TTO) and investigate the factors related to the postoperative progression of PFOA after patellar stabilization surgery.
Methods
Between 2009 and 2020, 30 consecutive patients underwent MPFLR with or without AMZ, using an autologous semitendinosus tendon graft; they were followed up for more than 2 years. iMPFLR was performed in cases of recurrent patellar dislocation with normal tibial tubercle-trochlear groove (TT-TG) distance and no PFOA, and MPFLR + AMZ was performed for cases of excessive TT-TG distance, preoperative PFOA of recurrent patellar dislocation, or habitual patellar dislocation. Clinical findings and radiographs of the PF joint were evaluated pre- and postoperatively with PF alignment parameters and PFOA and were compared between surgical procedures. Factors for the postoperative progression of PFOA were compared between the OA progression and non-progression groups.
Results
Postoperative clinical score, radiographic parameters except for sulcus angle, TT-TG distance, and progression of PFOA were not significantly different between the iMPFLR and MPFLR + AMZ groups. Postoperative lateral patellar displacement (p = 0.001) and congruence angle (p = 0.017) were significantly different between the OA progression and non-progression groups.
Conclusion
Similar to MPFLR for recurrent cases, MPFLR with AMZ can improve the clinical and radiographic outcomes in severe cases. The remaining parameters of patellar instability could be affected in the postoperative progression of PFOA after MPFL reconstruction with or without AMZ of TTO for patellar instability.
Design, synthesis and biological evaluation of novel 2- (indole arylamide) benzoic acid analogs as dual COX-2 / 5-LOX inhibitors
Authors: Du Le , Du Shuaishuai , li jiaming , Wang Hongwei 
Published: September 2022

Abstract
Recently, the drug discovery paradigm has evolved from single-target inhibition to a multi-target design concept. This study designed, synthesized, and evaluated a series of novel 2-(indole arylamide) benzoic acid analogs for their in vivo anti-inflammatory properties. Compounds 7f and 7n showed significant anti-inflammatory activity in a xylene-induced mouse model of auricular edema. Furthermore, 7f and 7n exhibited moderate COX-2 inhibitory activity (IC50 = 537 and 321.5 nM) than celecoxib (IC50 = 10.04 nM) in vitro, among which 7n had higher COX-2 selectivity activity (selectivity index (COX-1/COX-2) = 7.89) and moderate 5-LOX inhibitory activity (IC50 = 222.1 nM). Compared to zileuton (IC50 = 36.46 nM), compound 7f was identified as the most potent 5-LOX inhibitor (IC50 = 77.37 nM). According to the biological results, compounds 7f and 7n have better inhibitory activities on the production of NO and PGE2 in LPS-induced RAW 264.7 cell macrophages than celecoxib and indomethacin. As demonstrated by docking studies, 7f and 7n have stronger interactions with key residues in the active pocket of COX-1 or COX-2, which is consistent with the activity results. Based on these results, further research into safer and more effective anti-inflammatory drugs might be possible using 2-(indole arylamide) benzoic acid analogs.
Predicting adsorption behavior of 6-mercaptopurine anticancer drug upon polyaxazoline nanocarrier: A theoretical study
Authors: Rahmanifar Leila , Azarakhshi Fatemeh , Moniri Elham 
Published: September 2022

AbstractPredicting adsorption behavior of the 6-Mercaptopurine (MCP) anticancer drug upon the polyaxazoline nano-carrier was investigated using DFT and TD-DFT methods by B3LYP/6-31G* level in the gas phase and water solution. Based on the thermochemical parameters, the MCP/polymer complex in the solvent water is more stable rather than the gas phase. The adsorption energies of the MCP/polymer complex displayed that the adsorption process is exothermic. The UV/Vis absorption and IR spectra analysis were calculated to investigate the changes happening in the interaction of the MCP with polymer. FMO analysis indicated that the energy gap (Eg) of the polymer decreased after the adsorption process. Electronic properties and MEP analysis were also studied. Based on NBO analysis and charge difference (ΔN), the charge transfer in MCP/polymer occurs essentially from the polymer to the MCP drug, which is consistent with the results of NBO analysis. It is predicted that the POZ polymer can be used as a drug delivery system for MCP drug.
Yeast hydrolysate attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage in weaned piglet
Authors: Fu Runqi , Liang Chan , Chen Daiwen , Tian Gang , Zheng Ping , He Jun , Yu Jie , Mao Xiangbing , Luo Yuheng , Luo Junqiu , Yu Bing 
Published: September 2022

Abstract
Background
Yeast hydrolysate (YH) has attracted much attention as a nutritional additive, and it is generally considered to be non-toxic, effective and safe. However, there is still inconclusive on the effects of dietary YH supplementation on immunity function and intestinal barrier of piglets. This study was conducted to investigate the effects of YH supplementation on inflammatory responses and intestinal barrier in piglets challenged with lipopolysaccharide.
Methods
Twenty-four piglets (with an average body weight of 7.42 ± 0.34 kg) weaned at 21 days of age were randomly fed a diet containing 0 or 0.5% yeast hydrolysate for 21 days. On the 22nd day, half of piglets were intraperitoneally injected with lipopolysaccharide at 150 µg/kg BW, and the other half were injected with an equal volume of sterile normal saline. Four hours later, blood samples of each piglet were collected and then piglets were slaughtered.
Results
LPS injection increased serum levels of adrenocorticotropic hormone (ACTH), cortisol, haptoglobin (HP), serum amyloid A protein (SAA), tumor necrosis factor-α (TNF-α), and diamine oxidase (DAO) (P < 0.05), decreased the jejunal villus height, the protein and mRNA abundances of zonula occludens-1 (ZO-1) and occludin (OCLN) (P < 0.05), enhanced the protein and mRNA abundances of TNF-α, interleukin-1β (IL-1β), toll-like receptors 4 (TLR4) and phosphor-Nuclear factor-κB-p65 (p-NFκB-p65) (P < 0.01). Dietary YH supplementation attenuated the impaired effects of ACTH, cortisol, HP, IL-1β, and DAO in serum (P < 0.05). Furthermore, YH supplementation up-regulated the villus height, protein and mRNA abundances of ZO-1 and OCC (P < 0.05), downregulated TNF-α, IL-1β, TLR4 and p-NFκB-p65 mRNA expressions and the corresponding protein abundances in the jejunum in LPS-challenged pigs (P < 0.01).
Conclusion
YH could alleviate inflammatory response and intestinal barrier injury in weaned piglets challenged with LPS, which was associated with inhibited the TLR4/NF-κB signaling pathway.