bluuebunny/crossref_metadata_2025_split huggingface dataset:
Managing placenta accreta spectrum in low‐resource settings using a novel dissection‐free aorta clamp: Operative technique
Authors: Paily Vakkanal Paily , Sidhik Afshana , Girijadevi Raji Raj , Sudhamma Ajithakumari , Neelankavil Joshy Joseph , Menon Usha Govindankutty , George Raymond , Ramakrishnan Soumya , Cheriyan Sara , John Teena Eliz , Pradeep Manu 
Published: March 2022

AbstractObjectiveSurgical management of Placenta accreta spectrum (PAS) is associated with profuse bleeding and increased risk of operative injury to the adherent pelvic structures. We propose using a novel aorta clamp that can occlude the abdominal aorta without retroperitoneal dissection, thereby making it easy for an obstetrician‐gynecologist to use it. The novel Paily aorta clamp (PAC) is applied just above the bifurcation of the abdominal aorta.MethodsThis was a retrospective study of 33 women with varying grades of histopathology‐confirmed PAS, who were managed as an elective or emergency procedure in a tertiary center in India.ResultsTwenty‐nine women with advanced grades of PAS underwent sub‐total/total hysterectomies, while four women with low‐grade PAS underwent a conservative procedure. The procedures were associated with median estimated intra‐operative blood loss of 1000 ± 1500 ml, with only 21.2% (n = 7) requiring a transfusion of four or more units packed red blood cells. PAC was applied for a median of 55 ± 20 min and was not associated with any perioperative aortic wall injury or distal thromboembolic phenomenon.ConclusionOur experience using the novel PAC, in the current series and across multiple centers in India, demonstrates that the sizeable abdominal aorta can be clamped safely and effectively without retroperitoneal dissection—with no incidence of vascular injury so far. However, we would urge only designated centers with experienced obstetrician‐gynecologists—backed by a urologist, adequate blood bank and intensive care facilities—to tackle PAS procedures using the PAC technique.
Global resilience and new strategies needed for antimicrobial stewardship during the COVID‐19 pandemic and beyond
Authors: Goff Debra A. , Gauthier Timothy P. , Langford Bradley J. , Prusakov Pavel , Ubaka Chukwuemka Michael , Nwomeh Benedict C. , Yunis Khalid A. , Saad Therese , van den Bergh Dena , Villegas Maria Virginia , Martinez Nela , Morris Andrew , Ashiru‐Oredope Diane , Howard Philip , Sanchez Pablo J. 
Published: March 2022

AbstractResilience is having the ability to respond to adversity proactively and resourcefully. The coronavirus disease 2019 (COVID‐19) pandemic's profound impact on antimicrobial stewardship programs (ASP) requires clinicians to call on their own resilience to manage the demands of the pandemic and the disruption of ASP activities. This article provides examples of ASP resilience from pharmacists and physicians from seven countries with different resources and approaches to ASP—The United States, The United Kingdom, Canada, Nigeria, Lebanon, South Africa, and Colombia. The lessons learned pertain to providing ASP clinical services in the context of a global pandemic, developing new ASP paradigms in the face of COVID‐19, leveraging technology to extend the reach of ASP, and conducting international collaborative ASP research remotely. This article serves as an example of how resilience and global collaboration is sustaining our ASPs by sharing new “how to” do antimicrobial stewardship practices during the COVID‐19 pandemic.
Baicalein inhibits cell proliferation and enhances apoptosis in human A549 cells and benzo(a)pyrene‐induced pulmonary carcinogenesis in mice
Authors: Chandrashekar Naveenkumar , Subramanian Raghunandhakumar , Thiruvengadam Devaki 
Published: March 2022

AbstractOur current study is done to explore the possible mechanisms to elaborate on the growth inhibitory effect of baicalein (BE) in human lung carcinoma. Initially, BE (25 and 50 µM) treatment for 24 h, suppressed the viability and inhibited population growth in A549 cells. BE upholds the production of reactive oxygen species (ROS) with concomitant replenishment of glutathione, catalase, and glutathione peroxidase activity. The expression level of nuclear factor erythroid 2‐related factor 2 and heme oxygenase‐1 markedly increased after BE treatment will intimidate A549 cells proliferation by the ROS‐independent pathway via the antioxidant pathway. In vivo investigations were carried out on BE (12 mg/kg, oral) in benzo(a)pyrene (B(a)P; 50 mg/kg, oral) induced lung carcinogenesis in mice. BE induces caspase‐dependent apoptosis by increasing the levels of cytosolic cytochrome c accompanied by upregulating the outflow of p53, Bax, and caspase‐3 with a concomitant abatement in the outflow of Bcl‐2 in both in vitro and in vivo. In the murine model, BE treatment hindered the countenance of proliferation‐related proteins (argyrophilic nucleolar organizing regions and proliferating cell nuclear antigen). Additionally, appraisal of the cell nucleus by transmission electron microscopic assessment uncovered that BE treatment adequately counteracts B(a)P‐induced lung cancer cell survival. During the transition of the G0/G1 phase, BE is arrested in the cell cycle process. This might be the cause of a substantial increase in the appearance of p21Cip1 with concomitant downregulating the expressions of CDK4, cyclin D, and cyclin E both in vitro and in vivo. Our results conclude that BE treatment induced apoptosis and repressed proliferation both in vitro and in vivo of human lung carcinoma.
Audit quality, corporate governance, firm characteristics and CSR disclosures—Evidence from Indonesia
Authors: Handayati Puji , Tham Yeut Hong , Yuningsih Yuni , Rochayatun Sulis , Meldona  
Published: March 2022

AbstractThe purpose of this study is to investigate the impact of Big 4 auditors, corporate governance and firm characteristics on CSR disclosure in large listed firms in Indonesia adopting the stakeholder theory. The financial and corporate governance data are collected via the Indonesian Stock Exchange for the top 80 firms from 2016 to 2020 while data for CSR disclosures are hand collected from the annual report/sustainability report using the latest Global Reporting Initiative (GRI) 2020 index based on the environmental and social categories. The results suggest that reputable firms, diligent boards and firms audited by Big 4 auditors have a positive and statistically significant relationship with CSR disclosures. The study also find that firms with diligent board audited by Big 4 auditors are positively associated with CSR disclosures. This is the first study that utilises the latest GRI Index (2020) assessing the social and environmental disclosures in large listed firms in Indonesia over a longitudinal period.
Folding molecular dynamics simulation of T‐peptide, a HIV viral entry inhibitor: Structure, dynamics, and comparison with the experimental data
Authors: Gkogka Ioanna , Glykos Nicholas M. 
Published: March 2022

AbstractPeptide T is a synthetic octapeptide fragment, which corresponds to the region 185–192 of the gp120 HIV coat protein and functions as a viral entry inhibitor. In this work, a folding molecular dynamics simulation of peptide T in a membrane‐mimicking (DMSO) solution was performed with the aim of characterizing the peptide's structural and dynamical properties. We show that peptide T is highly flexible and dynamic. The main structural characteristics observed were rapidly interconverting short helical stretches and turns, with a notable preference for the formation of β‐turns. The simulation also indicated that the C‐terminal part appears to be more stable than the rest of the peptide, with the most preferred conformation for residues 5–8 being a β‐turn. In order to validate the accuracy of the simulations, we compared our results with the experimental NMR data obtained for the T‐peptide in the same solvent. In agreement with the simulation, the NMR data indicated the presence of a preferred structure in solution that was consistent with a β‐turn comprising the four C‐terminal residues. An additional comparison between the experimental and simulation‐derived chemical shifts also showed a reasonable agreement between experiment and simulation, further validating the simulation‐derived structural characterization of the T‐peptide. We conclude that peptide folding simulations produce physically relevant results even when performed with organic solvents that were not part of the force field parameterization procedure.
Exploratory assessment of serological tests to determine antibody titer against SARS‐CoV‐2: Appropriateness and limits
Authors: Colombini Alessandra , Viganò Marco , Tomaiuolo Rossella , Di Resta Chiara , Corea Francesca , Sabetta Eleonora , Ferrari Davide , De Vecchi Elena , Maria Spanò Sestina , Banfi Giuseppe 
Published: March 2022

AbstractBackgroundSerological tests can be used to detect antibodies in the serum of subject's after SARS‐CoV‐2 infection and vaccination. Currently, variability in antibody titers and the availability of a multiplicity of serological tests have made it necessary to highlight their appropriateness and limitations in various diagnostic settings.MethodsThis study is part of Covidiagnostix, a multicenter project aimed at the assessment of the health technology used in SARS‐CoV‐2 serological tests. Based on data gained from the analysis of over 5000 subjects, a selected number of serum samples, representative of different diagnostic settings, were analyzed first by qualitative immunoassays (IgA, M, and G MILLIPLEX® SARS‐CoV‐2 tests based on Luminex®) to define the immunoglobulins serum composition and subsequently by four serological diagnostic tests (Elecsys Anti‐SARS‐CoV‐2 and Elecsys Anti‐SARS‐CoV‐2 S by Roche, SARS‐CoV‐2 IgG by Siemens Healthcare, and CHORUS SARS‐CoV‐2 “NEUTRALIZING” Ab by DIESSE). The first WHO International Standard for SARS‐CoV‐2 was also analyzed using the same methods.ResultsThis study evaluated the antibody content and titer of the WHO Standard and serum of subjects with/without previous infection and before/after vaccination for SARS‐CoV‐2.ConclusionThe definition of antibodies in the WHO standard and the analysis of serum samples allowed for the identification of the appropriateness of serological tests in each diagnostic setting, increasing the effectiveness of the resulting laboratory data. Furthermore, we found that it would be optimal to produce new international standards against the S1 domain and RBD of the SARS‐CoV‐2 spike protein for a more effective serological monitoring of vaccination.
Circ_SAR1A regulates the malignant behavior of lung cancer cells via the miR‐21‐5p/TXNIP axis
Authors: Zhao Yi , Wan Ying , He Tianzhen 
Published: March 2022

AbstractBackgroundLung cancer is one of the most common malignancies globally and a significant component of cancer‐related deaths. The lack of early diagnosis accounts for detecting approximately 75% of cancer patients at an intermediate to an advanced stage, with a low 5‐year survival rate. Therefore, a more comprehensive understanding of the molecular mechanisms of lung cancer development is necessary to find reliable and effective therapeutic and diagnostic biomarkers.Methodscirc_SAR1A, miR‐21‐5p, and TXNIP in lung cancer tissues, animal xenografts, and cell lines were validated by qRT‐PCR and western blotting analyses. RNase R digestion and nuclear/cytoplasm fractionation experiments were utilized to determine the stability and localization of circ_SAR1A in lung cancer cells. The binding between miR‐21‐5p and circ_SAR1A or TXNIP was confirmed by luciferase reporter, RNA pull‐down, Spearman's correlation, and rescue assays. CCK‐8, colony formation, flow cytometry, Transwell, and western blotting were utilized to illustrate the malignant behavior of lung cancer cells.Resultscirc_SAR1A and TXNIP were down‐regulated while miR‐21‐5p was up‐regulated in lung cancer samples and cells. circ_SAR1A was located predominantly in the cytoplasm; it inhibited lung cancer growth in vitro and in vivo by sponging to miR‐21‐5p. miR‐21‐5p silencing suppressed lung cancer malignancy by targeting TXNIP.Conclusionscirc_SAR1A is a critical negative regulator of lung carcinogenesis. circ_SAR1A/miR‐21‐5p/TXNIP attenuation inhibited lung cancer progression, presenting an ideal diagnostic and a potential therapeutic target.
Biomarkers of connective tissue disease‐associated interstitial lung disease in bronchoalveolar lavage fluid: A label‐free mass spectrometry‐based relative quantification study
Authors: Ye Jing , Liu Pengcheng , Li Renming , Liu Hui , Pei Wenjing , Ma Changxiu , Shen Bing , Zhao Dahai , Chen Xiaoyu 
Published: March 2022

AbstractBackgroundThe pathogenesis of connective tissue disease‐associated interstitial lung disease (CTD‐ILD) is unclear. This study aims to identify differentially expressed proteins (DEPs) in CTD‐ILD to determine the potential role of these DEPs that may play in the pathogenesis of CTD‐ILD and to offer potential therapeutic targets.MethodsBronchoalveolar lavage fluid (BALF) samples were collected from four patients with CTD‐ILD and four patients without CTD‐ILD. Label‐free mass spectrometry‐based relative quantification was used to identify the DEPs. Bioinformatics were used to determine the potential biological processes and signaling pathways associated with these DEPs.ResultsWe found 65 upregulated DEPs including SFTPD, CADM1, ACSL4, TSTD1, CD163, LUM, SIGLEC1, CPB2, TGFBI and HGD, and 67 downregulated DEPs including SGSH, WIPF1, SIL1, RAB20, OAS3, GMPR2, PLBD1, DNAJC3, RNASET2 and OAS2. The results of GO functional annotation for the DEPs showed that the DEPS were mainly enriched in the binding, cellular anatomical entity, cellular processes, and biological regulation GO terms. The results of KEGG analyses showed that the pathways most annotated with the DEPs were complement and coagulation cascades, metabolic pathways, pathways in cancer, and PPAR signaling pathway. COG analyses further informed the functions associated with these DEPs, with most focused on signal transduction mechanisms; posttranslational modification, protein turnover, chaperones; intracellular trafficking, secretion, and vesicular transport; amino acid transport and metabolism; and lipid transport and metabolism.ConclusionsDEPs identified between patients with vs. without CTD‐ILD may play important roles in the development of CTD‐ILD and are potential new biomarkers for early diagnosis of CTD‐ILD.
microRNA‐126 inhibits vascular cell adhesion molecule‐1 and interleukin‐1beta in human dental pulp cells
Authors: Jiang Long , Krongbaramee Tadkamol , Lin Xinhai , Zhu Min , Zhu Yaqin , Hong Liu 
Published: March 2022

AbstractBackgroundVascular cell adhesion molecule (VCAM‐1) mediates pulpitis via regulating interleukin (IL)‐1β. microRNA (miR)‐126 was reported to regulate the VCAM‐1 under many different pathophysiological circumstances. We investigated variations of miR‐126 and VCAM‐1 in inflamed patient pulp tissues and determined potential roles of miR‐126 in pulpitis using human dental pulp cells (hDPCs) in vitro.MethodsWe quantitatively measured the transcripts of miR‐126 and VCAM‐1 in inflamed human pulp tissues using qRT‐PCR and compared with those from healthy human pulp tissues. In addition, we transfected miR‐126 in hDPCs using plasmid DNA (pDNA)‐encoding miR‐126 delivered by polyethylenimine (PEI) nanoparticles.ResultsThe irreversible pulpitis significantly reduced miR‐126 and increased the transcript of VCAM‐1 in pulp tissues (p < 0.05). pDNA‐encoding miR‐126 delivered PEI nanoparticles and effectively upregulated the expression of miR‐126 in hDPCs (p < 0.05). The overexpression of miR‐126 could effectively suppress the transcripts and protein levels of VCAM‐1 and IL‐1β induced by Pg‐LPS at 100ng/mL in DPCs (p < 0.05).ConclusionsmiR‐126 is involved in pulpitis and downregulated the VCAM‐1 and IL‐1β in DPCs. miR‐126 may be a potential target to attenuate the inflammation of pulpitis.
The contribution of skills to the effectiveness of dialectical behavioral therapy
Authors: Lee Rachael K. , Harms Craig A. , Jeffery Sian E. 
Published: March 2022

AbstractObjectiveThe present study investigated the effectiveness of dialectical behavioral therapy (DBT) and the effect of improvement in DBT skills on clinical outcomes.MethodParticipants included 57 adults who attended a community mental health service and underwent one of two modes of DBT. Twenty‐six adults had been diagnosed with borderline personality disorder (BPD) and participated in comprehensive DBT (DBT‐C; including group skills training, individual therapy, and phone coaching). Thirty‐one adults had BPD traits and participated in DBT skills training (DBT‐S; group skills training only). In the present study, the DBT skills examined were mindfulness, emotion regulation, and interpersonal effectiveness; the clinical outcomes examined were borderline symptoms, psychological distress, and quality of life.ResultsSix 2 × 2 analysis of covariances indicated significant improvements in psychological distress, quality of life, mindfulness, and interpersonal effectiveness (but not borderline symptoms and emotion regulation), over a 6‐month period. Mode of delivery of DBT did not impact on the improvement in DBT skills and clinical outcomes over the 6‐month period. Hierarchical multiple regression results indicated that improvement in DBT skills (mindfulness, emotion regulation, and interpersonal effectiveness) were associated with reductions in borderline symptoms and psychological distress, with emotion regulation the only skill uniquely associated with improvements in clinical outcomes.ConclusionsThe results of the present study support the effectiveness of DBT and the specific role of emotion regulation for favorable clinical outcomes over a 6‐month period, independent of the mode of delivery of DBT.
Patients' self‐perceived strengths increase during treatment and predict outcome in outpatient cognitive behavioral therapy
Authors: Schürmann‐Vengels Jan , Teismann Tobias , Margraf Jürgen , Willutzki Ulrike 
Published: March 2022

AbstractObjectiveModern conceptualizations suggest the independence of positive and negative mental health constructs. Research of positive constructs in psychotherapy is scarce. This study analyzed the development of patients' strengths during psychotherapy and whether pre‐therapy strengths incrementally predict treatment outcome.MethodsTwo hundred and two patients (56.44% female, mean age = 42.49) treated by 54 therapists underwent cognitive behavioral therapy. Patients' strengths in different contexts as well as psychopathology, interpersonal problems, and self‐esteem were assessed at the beginning and end of therapy.ResultsStrengths increased in the contexts of everyday life (EvdayS; d = 0.44, p < 0.001) and current problems (ProbS; d = 0.70, p < 0.001). Strengths in the context of previous crises that were managed successfully (CrisesS) did not change. However, baseline scores of CrisesS were a significant incremental predictor of all outcomes.ConclusionA differentiated assessment of positive constructs is useful for outcome prediction and the implementation of strength‐based interventions.
The role of self‐compassion in the mental health of adults with ADHD
Authors: Beaton Danielle M. , Sirois Fuschia , Milne Elizabeth 
Published: March 2022

AbstractObjectiveEvidence suggests that the poorer mental health associated with attention deficit hyperactivity Disorder (ADHD) is partially explained by adverse psychosocial correlates of the condition. As recent studies show that self‐compassion is negatively associated with ADHD, this study investigates if levels of self‐compassion may explain the mental health outcomes in people with ADHD compared to people without ADHD.MethodA total of 543 adults with ADHD (62.72% female, 18–67 years), and 313 adults without ADHD (66.45% female, 18–82 years) completed questionnaires online to measure levels of self‐compassion and mental health. A Structural Equation Model assessed the mediating effect of self‐compassion on the relationships between ADHD and well‐being (psychological, emotional, and social), and ADHD and ill‐being (depression, anxiety, and stress).ResultsFindings suggest that low self‐compassion contributes to poorer mental health in adults with ADHD compared to adults without ADHD.ConclusionsThus, self‐compassion may be a potential target to improve mental health in this population.Public Health SignificanceThis study shows that self‐compassion is an important factor in the mental health of adults with ADHD and provides preliminary evidence for the use of self‐compassion interventions to improve mental health outcomes in adults with ADHD.
A small nucleolar RNA, SNORD126, promotes adipogenesis in cells and rats by activating the PI3K–AKT pathway
Authors: He Yi , Wu Yu , Mei Bin , Fang Xianlong , Cai Guangzhen , Cai Ning , Wu Qiqi , Huang Zhao , Ge Chang , Liang Huifang , Zhang Bixiang , Chen Xiaoping , Chu Liang 
Published: March 2022

AbstractSmall nucleolar RNA (snoRNA) plays important role in various histogenesis. Whether snoRNA plays a role in adipogenesis is unknown. SNORD126 is a C/D box snoRNA. We previously demonstrated that SNORD126 promoted hepatocellular carcinoma cell growth by activating the phosphoinositide 3‐kinase–protein kinase B (Akt) pathway through upregulating fibroblast growth factor receptor 2 expression. In the present study, we found that the expression of SNORD126 was downregulated in the obesity‐related tissues in high‐fat diet‐fed rats. Overexpression of SNORD126 in 3T3‐L1 cells promoted adipocytes differentiation. SNORD126 significantly increased the expression of CCAAT/enhancer‐binding protein α, fatty acid‐binding protein 4, peroxisome proliferative‐activated receptor‐γ, and the phosphorylation of Akt and p70S6K. Overexpression of SNORD126 in human adipose‐derived stem cells stimulated adipogenesis and increased phosphorylation of Akt. Meanwhile, SNORD126 increased the messenger RNA and protein levels of cyclin D1 and cyclin‐dependent kinase 2, which promoted mitotic clonal expansion progression during the early stage of 3T3‐L1 cell differentiation. We further found that SNORD126 accelerated the growth of the groin fat pad and increased phosphorylation of Akt and p70S6K in rats. Overall, our results suggested that SNORD126 promoted adipocyte differentiation through increasing phosphorylation of Akt and p70S6K both in vitro and in vivo.
Superior polysulfide adsorption ability by using Ti3C2 nanosheets as effective reservoirs for high electrochemical performance
Authors: Luo Jie , Zheng Jianming 
Published: March 2022

AbstractBACKGROUNDA large amount of research has improved electrochemical performance by strengthening the chemical affinity by using polar materials in lithium–sulfur batteries. However, only some of this research has focused on the relationship between structure and polysulfide adsorption ability.RESULTSIn this work, a Ti3C2 nanosheet structure has been applied as sulfur host matrix to improve electrochemical performance. Due to the nanosheet structure, the polysulfide was able to diffuse between the Ti3C2 layers, therefore improving the catalytic effect of the polysulfide. Moreover, the space between the Ti3C2 layers can store the soluble polysulfide as an effective reservoir. As a result, the traditional shuttle effect of the polysulfide could be efficiently inhibited.CONCLUSIONTherefore, the as‐prepared Ti3C2/S composites exhibited high specific capacity, high rate performance, and stable cycle performance. All these superior electrochemical performances are attributed to the presence of the Ti3C2 nanosheet. © 2022 Society of Chemical Industry (SCI).