bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Dose Assessment of 68Ga-NODAGA-RGD-BBN Heterodimer Peptide based on Animal Data
Authors: Amraee Naeimeh , Alirezapour Behrouz , Hosntalab Mohammad , Yousefnia Hassan 
Published: November 2022

Aims:
Calculation of the absorbed dose in human organs is one of the first steps for developing new radiopharmaceuticals. The aim of this study is to estimate the human absorbed dose of a newly developed 68Ga-NODAGA-RGD-BBN radiolabeled compound.

Materials and Methods:

68Ga-NODAGA-RGD-BBN was prepared by varying different parameters at optimized conditions. The stability of the radiolabeled peptide in phosphate-buffered saline (PBS) and in human serum was evaluated for 120 min. Afterward, the biodistribution of the complex was assessed in normal and tumor-bearing mice, at least for 120 min postinjection. Finally, the human absorbed dose of 68Ga-NODAGA-RGD-BBN was estimated based on mice data using Radiation Dose Assessment Resource and Spark method.

Results:

68Ga-NODAGA-RGD-BBN was produced with radiochemical purity of more than 98% (high-performance liquid chromatography/ radio thin layer chromatography (RTLC)) with high stability in PBS buffer and in human serum at least for 2 h. The complex demonstrated high uptake in gastrin-releasing peptide receptor-expressing tumors compared to other nontarget organs. Furthermore, the dose assessment for the complex showed that the kidneys receive the highest absorbed dose in comparison with other organs.

Conclusion:
The result of this study showed that 68Ga-NODAGA-RGD-BBN is an effective and radiolabeled ligand for tumor detection, however more studies are still needed.
Calculation of Skin Dose Rate Conversion Factors Due to Surface Contamination from Frequently used Radionuclides in Local Nuclear and Medical Facilities
Authors: Pasquevich Ian , Velasco Fiorela Merma , Andres Pablo 
Published: November 2022

Purpose:
The use of nonsealed radioactive sources can lead to skin contamination due to radiological accidents and staff oversight. This contamination has been shown to contribute considerably to the total skin dose received by nuclear medicine technicians and can easily exceed the limit of 500 mSv/year established by the current regulations. To assess the severity of contamination, it is necessary to estimate the skin dose through the use of suitable skin dose rate conversion factors. To determine the appropriate factors, it is important to study the influence of the contamination area, the epidermal thickness, and the percutaneous absorption on them.

Materials and Methods:
Monte Carlo simulations using the code PHITS 3.02 were carried out to study and quantify the dosimetry conversion factors of 15 frequently used radionuclides (11C, 18F, 36Cl, 54Mn, 60Co, 90Sr, 99
mTc, 123I, 131I, 137Cs, 153Sm, 177
Lu, 223Ra, 226Ra, and 241Am).

Results:
The absorbed dose to the skin is significantly influenced by epidermal thickness and percutaneous absorptions and can differ by up to two orders of magnitude with respect to the operational magnitude H'(0.07,0°).

Conclusions:
Skin dosimetry after a contamination incident may be complex because the absorbed dose delivered to the basal layer is influenced by the contamination area, the epidermal thickness, and the percutaneous absorption. Therefore, when an accident occurs, the dose should be quantified taking into account these parameters, especially the epidermal thickness, and the possible percutaneous absorption should be evaluated in cases where the contamination involves a dose approximately equivalent to the established limits.
Anti-Inflammatory, Viral Replication Suppression and Hepatoprotective Activities of Bitter Kola - Lime Juice, - Honey Mixture in HBeAg Seropositive Patients
Authors: Olaniyan Mathew Folaranmi , Uwaifo Ferdinand , Olaniyan Tolulope Busayo 
Published: November 2022

Study Background:
Bitter kola (Garcinia kola), lime juice, and honey mixture have some traditional but nonscientific applications in the treatment of some infectious diseases due to the phytochemical and phytonutrient components that may have antidiabetic, anti-inflammatory, antipyretic, immunomodulatory, antiatherogenic, antimicrobial, purgative and antiparasitic activities while expression of hepatitis B envelope antigen (HBeAg) is an indication of active hepatitis B virus replication and a high degree of infectiousness.

Aim and Objective:
This work was therefore designed to determine anti-inflammatory response, possible suppression of active viral replication and liver damage in HBeAg seropositive patients who used bitter kola, lime juice and honey mixture.

Materials and Methods:
Out of 39 hepatitis B surface antigen and HBeAg seropositive patients who volunteered themselves for the study only 23 volunteers (23; Male: 13; Female: 10; age 34–56 years) were successfully monitored. The mixture is prepared by mixing grounded bitter kola (30 seeds) with 500 ml of Lime Juice and 500 ml of honey. The mixture was kept for 5 days before administration. The mixture was taken by subjects at 4 divided dose for 2 months using 4 teaspoonfuls per dose. Plasma alanine transaminase (ALT) was measured using COBAS C111 autoanalyzer, plasma interleukin-10 (IL-10), Antibodies against hepatitis C virus (anti-HCV), HBeAg, anti-HBeAg, p24 ag-ab by enzyme-linked immunosorbent assay while acid-fast bacilli and Plasmodium were determined in the subjects by microscopic examination of stained smears.

Results:
The frequency of HBeAg (26.1% [6]) in the patients after the use of bitter kola, lime juice, and honey mixture was lower than the frequency of the viral protein (HBeAg 100% [23]) before the use of the mixture. None of the HBeAg seropositive patients studied expressed anti-HBeAg before the use of bitter kola, lime juice, and honey mixture but 56.5% (13) of the patients expressed anti-HBeAg while 17.4% (4) co-expressed HBeAg + anti-HBeAg after the use of the mixture. There was a significant increase in the plasma value of IL-10 and a significant decrease in the plasma value of ALT in the HBeAg seropositive patients after the use of bitter kola, lime juice, and honey mixture (P < 0.05).

Conclusion:
Bitter kola, lime juice, and honey mixture could have anti-inflammatory, active viral replication, and liver damage suppression bioactivities in HBeAg seropositive patients as indicated by decrease in HBeAg, expression of anti-HBeAg, decrease in plasma ALT and increase in IL-10 after the use of the mixture.
Prevalence and Predictors of Relapse among Patients with Schizophrenia at Psychiatric Healthcare Institutions in North-Western Nigeria
Authors: Balarabe F. , Gommaa H.I.M. , Abdellatif S.A. , Mohammed A. 
Published: November 2022

Background: Nigerians with Schizophrenic disorder are experiencing multiple relapses during the course of the illness. Relapse is disabling and distressing, associated with progressive functional deterioration as well as worsening treatment response and clinical prognosis.
Aim: Thestudy aims to assess the prevalence and predictors of relapse among patients with Schizophrenia in North-western Nigeria.
Methods: A cross-sectional descriptive design was employed to conduct the study in Federal Neuro-Psychiatric Hospital Kaduna, Kano State Psychiatric Hospital Dawanau, and Federal Neuro-Psychiatric Hospital Kware, Sokoto States. The Population of the study constitutes all patients with schizophrenia attending the outpatient units of the Hospitals with a sample size of 464 patients a crossed the facilities. A purposive sampling technique was used in the study to recruit respondents. Self -structure Questionnaire was the instrument for data collection. Data collected were analyzed using SPSS Version 23 and presented using mean, standard deviation, frequency, and percentages. Regression analysis was used at a 0.05 level of significance to determine predictors of relapse..
Results: Findings revealed that from the year 2018 to 2020, the prevalence of relapse among patients with schizophrenia is very high in the year 2020 with 71% relapse in Kano state, 40% relapse in Sokoto state, and 14% relapse in Kaduna state. The study shows that co-morbid illness, stressful life events, and inadequate mental health services are predictors of relapse (p<0.05).
Conclusion: The study concludes that personal, social, and health facility factors are predictors of relapse among patients with Schizophrenia in North-west Nigeria.
Recommendations: Emotional and social support like halfway home, assertive community therapy, self -help groups should be provided to patients with schizophrenia, as these can play a vital role in their response to the ailment thereby reducing relapse and the prevalence of the disease.
Réponse cardio-respiratoire de sujets sains au repos avec et sans port de masque: résultats préliminaires
Authors: Kouassi Michel Brou , Kouame Appolinaire Brou , Kondo Liliane , Teniloh Yeo Augustin , Yapo Paule-Denise , Djaha-Toumata Kossiwa , Toowlys Aristide , Balayssac-Siransy Edwige , Ouattara Soualiho , Dah Cyrille , Bogui Pascal 
Published: November 2022

Parmi les dispositions prises par les états pour lutter contre la pandémie de la Covid-19, le port de masque est un moyen recommandé et imposé dans la plupart des pays. La vulgarisation du port de masque a eu pour corollaire un nombre important d’utilisateurs publics dont certains manifestent un inconfort et des signes cliniques liés à ce dispositif. Le but de ce travail préliminaire a été d’évaluer au repos, la réponse cardio-respiratoire aigue liée au port de masque chez des sujets sains. Une étude expérimentale, prospective, transversale à visée descriptive incluant 36 volontaires a été réalisée. Un protocole organisé en deux étapes sur deux jours distincts a été exécuté par les volontaires. La pression partielle de gaz carbonique en fin d’expiration (PetCO2), la Fréquence Respiratoire (FR), la saturation périphérique en oxygène et la fréquence cardiaque ont été mesurés avant et après le port d’un masque chirurgical de marque Solumask® type II - NF EN 14683, chaque minute pendant cinq minutes consécutives. Les moyennes de PetCO2 et de FR aux différentes minutes étaient significativement plus élevées avec le port de masque. Lors d’un port prolongé du masque, le renouvellement inefficace du gaz alvéolaire atténuerait la teneur du sang en oxygène. Cela pourrait expliquer l’inconfort et les manifestations cliniques. Une approche consistant à l’évaluation de la réponse cardio-respiratoire à l’effort devrait être envisagée.
Among the state measures to control the Covid-19 pandemic, the usage of masks is a recommended and imposed method in most countries. The popularization of mask use has resulted in a large number of public users, some of whom are experiencing discomfort and clinical symptoms related to this device. The aim of this preliminary work was to evaluate the acute cardiorespiratory response to mask use among healthy subjects at rest. An experimental, prospective, cross-sectional, descriptive study including 36 volunteers was performed. A protocol organized in two steps on two separate days was performed by the volunteers. The end-tidal pressure of carbon dioxide (PetCO2), the Respiratory Rate (RR), the peripheral oxygen saturation and the heart rate were measured before and after wearing a Solumask® model II - NF EN 14683 mask, every minute for five consecutive minutes. The mean PetCO2 and RR at individual minutes were significantly higher with mask use. During prolonged mask wear, inefficient alveolar gas turnover would attenuate the oxygen content of the blood. This could explain the discomfort and clinical manifestations. An approach consisting of the evaluation of the cardiorespiratory response to exercise should be considered.
Evaluation des niveaux de contamination bactériologique de la laitue selon les sites de production et de vente dans les sites de maraîchage d’Abidjan et zone-périurbaine
Authors: Wognin Affou Séraphin , Ouattara Mohamed Baguy , Assi-Clair Brice Judicaël , Koffi-Nevry Rose 
Published: November 2022

Cette étude, avait pour but l’évaluation du niveau de contamination bactériologique de la laitue vendue à Abidjan. Elle s’inscrit dans le cadre d’une contribution à la surveillance sanitaire des légumes consommables crus. Elle a été conduite afin d’établir une base de données sur les souches témoins de contamination fécales de l’environnement maraîcher d’Abidjan en fonction des saisons. Pour ce faire, 130 et 48 échantillons de laitue ont été respectivement prélevés sur trois grands sites de production et un site de vente à Abidjan durant quatre (04) saisons. Les résultats ont montré que quel que soit le site et la saison, les charges des indicateurs de contamination à savoir les coliformes fécaux (2,9×10x3 à 4×104 UFC/g), Clostridium perfringens (3,1×102 à 1,6×103 UFC/g), les entérobactéries (1×105 à 1,9×107 UFC/g) et Staphylococcus aureus (8,3×103 à 5,6×104 UFC/g) sont largement supérieures aux valeurs limites établies par l’Association Française de Normalisation (AFNOR) pour les légumes susceptibles d’être consommés crus. En outre, les laitues prélevées sur le site de vente ont été plus contaminées par les entérobactéries que celles obtenues des sites de production. Les charges élevées en souches témoins de contamination fécale pourraient induire la présence de germes pathogènes dangereux pour les consommateurs. Il est donc nécessaire d’étudier les espèces pathogènes dans le maraîchage périurbain d’Abidjan.
This study aimed to evaluate the level of bacteriological contamination of lettuce sold in Abidjan. It was conducted in order to establish a database on the control strains of fecal contamination of the market garden environment of Abidjan according to the seasons. To do so, 130 and 48 samples of lettuce were respectively collected from three major production sites and one sales site in Abidjan during four (04) seasons. The results showed that whatever the site and the season, the loads of contamination indicators, namely faecal coliforms (2.9×103 to 4×104 CFU/g), Clostridium perfringens (3.1×102 to 1.6×103 CFU/g), enterobacteria (1×105 to 1, 9×107 CFU/g) and Staphylococcus aureus (8.3×103 to 5.6×104 CFU/g) are well above the limit values established by the Association Française de Normalisation (AFNOR) for vegetables that can be eaten raw. In addition, lettuces collected from the sales site were more contaminated with Enterobacteriaceae than those obtained from the production sites. The high loads of fecal contamination control strains could induce the presence of pathogenic germs dangerous to consumers. It is therefore necessary to study the pathogenic species in peri-urban market gardening in Abidjan.
Détermination des facteurs de rendement et de la composition nutritionnelle de plats sénégalais à base de riz
Authors: Ndao Diao Sokhna , Mbaye Arame , Niang Ndeye Diakhou Djitté , Fall Magatte faye , Fall Dioum Seynabou , Diop Amadou , Ndiaye Bara , Diop Yérim M. , Sarr Serigne Omar 
Published: November 2022

Le lien entre l’alimentation et la santé étant scientifiquement établi, pour évaluer les apports et risques nutritionnels liés à l’alimentation sénégalaise, il est indispensable de connaitre la composition nutritionnelle des plats locaux à base de riz consommés quotidiennement au déjeuner par plus de 86% des sénégalais. La composition nutritionnelle des plats préparés peut être déterminée soit par analyse directe, soit par calcul à partir des facteurs de rendement et de rétention. D’où l’objectif de cette étude qui consiste à évaluer par approche directe, la capacité des plats à base de riz prélevés dans les ménages et/ou préparés au laboratoire, à couvrir les besoins nutritionnels des sénégalais et à déterminer leurs facteurs de rendement. Ainsi, le riz au poisson rouge, le riz au poisson blanc, le « mafé » au gombo, le « yassa » au poisson, couvrent 8 à 22% des besoins en fibres, 48 à 100% de ceux en zinc, 43 à 188% de ceux en fer, 17 à 114% de ceux en calcium, 55 à 77% de ceux en magnésium; 82% de ceux en lipides ; 319% des apports recommandés en sel et 108% en sucres totaux. Quant aux facteurs de rendement, ils varient entre 0,72 et 1,83.
The link between food and health being scientifically established, to assess the nutritional intake and risks associated with the Senegalese diet, it is essential to know the nutritional composition of the local rice dishes consumed daily at lunch by more than 86% of Senegalese. The nutritional composition of prepared meals can be determined either by direct analysis, or by calculation based on yield and retention factors. Hence the objective of this study which consisted in evaluating by direct approach, the capacity of rice dishes taken from households or prepared in the laboratory, to cover the nutritional needs of Senegalese and to determine their yields factors. Thus, red rice with fish, white rice with fish, "mafé" with okra, "yassa" with fish, cover 8 to 22% of fiber needs, 48 to 100% of zinc needs, 43 to 188% of those in iron, 17-114% of those in calcium, 55-77% of those in magnesium; 82% of those in lipids; 319% of recommended salt intake and 108% of total sugars. As for the yield factors, they vary between 0.72 and 1.83.
Trifolirhizin relieves renal injury in a diabetic nephropathy model by inducing autophagy and inhibiting oxidative stress through the regulation of PI3K/AKT/mTOR pathway
Authors: Xin Yunyi , Xu Xiao , Yang Xufeng , Chen Yufan , Zhu Danning 
Published: November 2022

Purpose: To evaluate the effects of trifolirhizin on diabetic nephropathy (DN), and the mechanism of action.
Methods: Male db/db mice (8 weeks, n = 24) and age-matched control mice (n = 6) were obtained. The mice were further divided into four groups and administered increasing doses of trifolirhizin (0, 12.5, 25 and 50 mg/kg). Histological analysis of renal tissues were performed by H & E staining. Blood urea nitrogen (BUN) and creatinine were determined using enzyme-linked immunosorbent assay (ELISA). Immunoblot and TUNEL assay were performed to investigate the effect of trifolirhizin on autophagy and apoptosis, while ELISA and dihydroethidium (DHE) staining were conducted to evaluate reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) levels. The effect of trifolirhizin on PI3K/AKT/mTOR pathway was determined using Immunoblot assays.
Results: Trifolirhizin alleviated renal injury in diabetic mice, and also activate autophagy and inhibited apoptosis in the renal tissues in diabetic mice (p < 0.001). In addition, trifolirhizin inhibited the oxidative stress response in the renal tissue in diabetic mice (p < 0.001). Trifolirhizin further inhibited PI3K/AKT/mTOR pathway and therefore relieved renal injury in the diabetic nephropathy model (p < 0.001).
Conclusion: Trifolirhizin alleviates renal injury in diabetic mice, activates autophagy, and inhibits apoptosis in renal tissue of diabetic mice. Therefore, trifolirhizin is a promising a promising drug for the treatment of DN.
Captopril reverses chronic unpredictable mild stress-induced depression-like behavior in rats via bradykinin-B2r signaling pathway
Authors: Zhang Shaofeng , He Lin 
Published: November 2022

Purpose: To investigate the effect of captopril on chronic unpredictable mild stress (CUMS)-induced depression-like behavior in mice, and the involvement of the bradykinin-B2r signaling pathway in the process.
Methods: Sixty healthy male C57BL/6J mice were assigned to control, model and high-, medium- and low-dose captopril groups and given the drug at doses of 9, 18 and 36 mg/kg, respectively. Open field and elevated cross maze tests were carried out, and escape latency in Morris water maze test was also test. The expressions of bradykinin B2R signal pathway proteins were assayed.
Results: Open arm residence time and open arm entry times were significantly higher in captopril-exposed mice than in model mice, while 5-day escape latency values were significantly less in captopril-treated mice than in model group (p < 0.05). Protein expressions of B2R, bpnf and Cdc42 in captopril groups were significantly higher than those in model group (p < 0.05).
Conclusion: Captopril mitigates CUMS-mediated depression-like disease in mice by regulating bradykinin B2R signal pathway. Therefore, captopril may play an antidepressant role by activating the expressions of B2R, bpnf and Cdc42.
LncRNA-ATB inhibits the proliferation and invasion of NSCLC cells by regulating MiR-200s expression
Authors: Song Liping , Zhao Can , Zhou Yujiao , Lei Tao , Cao Jianguang , Zhu Min 
Published: November 2022

Purpose: To investigate the effect of lncRNA-ATB on the proliferation and invasion of non-small cell lung cancer (NSCLC) cells, and its mechanism of action.
Methods: LncRNA-ATB mRNA levels in carcinoma tissues and normal adjacent tissues of 38 NSCLC patients in Peking University Shougang Hospital were determined by reverse transcription-polymerase chain reaction (RT-PCR). Human NSCLC A549 cell line was divided into control and lncRNA-ATB inhibition (si-ATB) groups, respectively. The proliferation and invasion of cells in each group were assessed. Subsequently, the effect of lncRNA-ATB inhibition on the growth of NSCLC cells was evaluated by subcutaneous tumor formation assay.
Results: The expression of lncRNA-ATB was significantly higher in carcinoma tissues than in normal adjacent tissues in NSCLC patients. Cell counting kit-8 (CCK-8) assay results showed that si-ATB group displayed a weakened ability of the cells to proliferate (p < 0.05). Furthermore, deoxyribonucleic acid (DNA) replication ability was weaker in si-ATB group than in the control group. Wound healing assay results showed that the migration ability of cells in the si-ATB group was lower than that in the control group. Also, lncRNA-ATB knockdown inhibited the invasion ability of human NSCLC cells (p < 0.05). Tumor formation assay data indicate that lncRNA-ATB knockdown significantly repressed the subcutaneous tumor formation ability of NSCLC cells. Furthermore, lncRNA-ATB knockdown in NSCLC cells up-regulated miR-200a, miR-200b and miR-200c.
Conclusion: The expression level of LncRNA-ATB is elevated in carcinoma tissues of NSCLC patients, and its knockdown suppresses the proliferation and invasion of NSCLC cells by up-regulating miR-200s. This finding suggests that it is a potential strategy for the management of NSCLC patients.
Proteasome activator 28A: A clinical biomarker and pharmaceutical target in acute cerebral infarction therapy
Authors: Guo Cuiping , Han Jianghong , Xu Jie , Yuan Zhiyao , Li Dan , Liu Qiang , Wang Zhenghui 
Published: November 2022

Purpose: To determine the dynamic changes in serum levels of PA28α in patients with acute cerebral infarction (ACI), and to investigate its correlation with infarct size and neurological deficit of the disease.
Methods: A total of 100 ACI patients and 100 healthy volunteers were recruited from The First Affiliated Hospital of Xinxiang Medical University as case and control groups, respectively. Their serum levels of PA28α were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The potential of PA28α in predicting the incidence of ACI was assessed by plotting ROC curves. Multivariate logistic regression analysis was conducted to investigate the risk factors of ACI. In addition, an ACI model in rats was established, and ACI rats were classified into 1, 3, 5, 7 and 14 day subgroups based on the duration post-ACI. Rats in the sham group served as control.
Results: Serum level of PA28α was significantly higher in ACI patients than in controls. Moreover, the serum level of PA28α at admission was positively correlated to the NIHSS score and infarct volume of ACI patients. The level of PA28α in ACI rats gradually increased post-ACI, reaching a peak on day 7. The number of apoptotic brain cells in ACI rats gradually decreased after ACI. In addition, PA28α level was negatively correlated to the number of apoptotic brain cells in ACI rats (R2 = 0.5148, p < 0.001).
Conclusion: The serum level of PA28α is elevated in ACI patients, and is positively correlated to infarct volume and neurological deficit of the disease. The dynamic change in brain cell apoptosis post-ACI is negatively correlated to the serum level of PA28α. These findings may provide theoretical basis for the diagnosis and treatment of ACI.