bluuebunny/crossref_metadata_2025_split huggingface dataset:
Luminescence mechanisms in the 2V2O5-xLi2O-(98-x)B2O3 glass matrices developed for creation of efficient glass-ceramic materials
Authors: Chukova Oksana , Nedilko Serhii G. , Voitenko Tetiana , Minikayev Roman , Paszkowicz Wojciech , Stasiv Vasyl , Zhydachevskyy Yaroslav , Suchocki Andrzej 
Published: November 2022

Abstract
The oxide glass-ceramics are promising class of solid state materials because they are using thermally stable and chemically inert glass oxide matrices. Development of such efficient glass matrices suitable for creation of glass-ceramic materials for several purposes is an important practical task. The xLi2O-yV2O5-(100-x-y)B2O3 undoped glass and 47Li2O-2V2O5-50B2O3-1La0.3Eu0.7VO4 glass samples with crystalline nanoinclusions were synthesized and investigated using XRD, IR and UV-Vis spectroscopy and UV band-to-band excitation of luminescence. The synthesized glass samples are characterized by wide band photoluminescence emission with maximum at 570 nm and intensity increased with increase of Li2O concentration. The excitation spectra consist of three bands with maxima positions at 270, 320 and 365 nm. The observed concentration dependencies of spectral distributions in the absorption and excitation spectra are explained by influence of the lithium ions on a ratio between triborate and tetraborate groups in the glass networks. The assumption is made that the observed wide band photoluminescence emission of the glass matrix can appear as a result of recombination processes between the defects in borate networks and the broken vanadate groups. The crystalline component in the doped glass samples is found to not affect the luminescence properties of the glass matrix. Intensity of narrow band photoluminescence emission of the crystalline component is up to 10 times more intense than that of the glass matrix wide band emission. The synthesized type of the glass matrices has promising characteristics for the use of developed materials in lighting devices, as it allows improving the spectral distribution of light emission towards the white light.
Analysis of Risk Factors Associated with the High Incidence of Amblyopia in Preterm Infants at the Corrected Gestational Age of 12 Months
Authors: Cao Yiwen , Wang Ying , Li Bian , Zhu Dehai , Sang Tian , Du Xueyan , Shi Wanjun , Yang Liu 
Published: November 2022

Abstract
Objective
To investigate the perinatal and in-hospital risk factors associated with the high incidence of amblyopia in preterm infants and to analyze the correlation between the amblyopia risk factors (ARFs) and neurodevelopment.
Methods
Children discharged from the neonatal intensive care unit (NICU) at 12 months of corrected gestational age were retrospectively included in this study. Ocular screening was performed in children. The presence of ARFs was determined according to the American Academy of Ophthalmology Guidelines for automated preschool vision screening factors. Differences in perinatal characteristics, complications during hospitalization, and treatment modalities between the two groups of children were analyzed, and multifactorial logistic regression analysis was used to identify the independent risk factors for ARFs. The results of developmental assessment were collected retrospectively to analyze the correlation between ARFs and various aspects of neurological development.
Results
A total of 128 preterm infants, 30 in the ARF group and 98 in the non-ARF group, were included in this study. Univariate analysis showed that the ARF group had lower birth weights, higher rates of asphyxia, preterm brain white matter injury, bronchopulmonary dysplasia (BPD), intraventricular hemorrhage (IVH), sepsis during hospitalization, and higher rates of treatment with pulmonary surfactant (PS), blood transfusion, invasive ventilator, and levothyroxine. Logistic regression analysis showed that BPD in the neonatal period (odds ratio [OR] 8.355, 95% confidence interval [CI] 1.492, 46.786), brain white matter injury (OR 16.742, 95% CI 0.684, 409.804), treatment with levothyroxine (OR 2.859, 95% CI 0.946, 8.639), and use of an invasive ventilator (OR 2.983, 95% CI 0.942, 9.445) were independent risk factors for amblyopia at 12 months of corrected gestational age, while the administration of glucocorticoids (OR 0.055, 95% CI 0.004, 0.737) was a protective factor. Regarding neurodevelopmental assessment, the number of infants with lagging fine motor development was greater in the ARFs group.
Conclusion
The presence of BPD in the neonatal period, brain white matter damage in preterm infants, and use of levothyroxine and invasive ventilator were high risk factors for ARFs. The use of glucocorticoids therapy was a protective factor. Children with ARFs had a higher rate of poor fine motor development.
Is remote ischaemic preconditioning worthwhile for patients undergoing cardiac surgery with cardiopulmonary bypass? A meta-analysis and systematic literature review
Authors: Yao Yangyang , Kadeer Mujiesemu , Askar Madina , Qinggusi Maolieen , Qiu Bingqi , Chen Chunling 
Published: November 2022

Abstract
Background: Numerous randomized controlled trials (RCTs) investigated the clinical benefits of remote ischemic preconditioning (RIPC) prior to cardiopulmonary bypass (CPB). However, to date, there is still no consensus on the benefit of this procedure. Therefore, this meta-analysis aimed to evaluate the treatment outcomes of RIPC in patients treated with CPB.
Methods: Databases were systematically searched for eligible RCTs comparing the delivery of RIPC with no treatment or sham RIPC (control) before CPB. Two authors individually screened studies and assessed the risk of bias. The primary outcome measure was mortality. The secondary outcome measures were length of intensive care unit (ICU) or hospital stay, the incidence of myocardial injury (MI), low cardiac output syndrome (LCOS) and so on. The relative risk (RR) and the population size mean difference (SMD) at the 95% confidence interval (95%CI) were compared between the 2 groups.
Results: Forty-one RCTs met the eligibility criteria. RIPC did not reduce overall and in-hospital mortality compared with the control group (RR=0.99, 95%CI: 0.78-1.26, I2=12%). No remarkable difference was observed between the two groups regarding the length of ICU and hospital stay (SMD: -0.12, 95%CI: -0.29−0.06, I2=93%; SMD: -0.926, 95%CI: -2.103−0.251, I2=95.2%, respectively). However, RIPC reduced the incidence of MI and the ventilation duration slightly when compared to the control group (RR=0.86, 95%CI: 0.76−0.99, I2=22%; RR=-0.16 95%CI: -1.85−1.52, I2=0%, respectively).
Conclusion: RIPC did not have an impact on mortality and length of ICU/hospital stay, however, it reduced the incidence of MI and the ventilation duration. This work does not recommend RIPC for patients underwent cardiac surgery.
Privacy-Preserving Cloud Data Sharing for Healthcare Systems with Hybrid Blockchain
Authors: . Raghav , Andola Nitish , Venkatesan S. , Verma Shekhar 
Published: November 2022

Abstract
Privacy-preserving sharing of cloud data requires user authentication and fine-grained access control along with privacy provisioning mechanisms. In this paper, we propose two schemes, keyword-based re-encryption (KRE) scheme and oblivious transfer-based re-encryption (OTRE) scheme for privacy-preserving secure health data sharing. KRE preserves data privacy and provides fine-grained access control with reduced key management overhead. OTRE is a superset of KRE that utilizes OT for users' data privacy and multiple data sharing. The schemes are collusion resistant against an honest but curious cloud and malicious users. The use of hybrid blockchain removes the need for a single trusted authority and provides large-scale secure data sharing. Blockchain also enables authentication between end-users and verifiability of data on the cloud through smart contracts. We implemented the schemes using the SageMath tool and hybrid blockchain using Go-Ethereum with Remix. The overall average time consumption of KRE scheme and OTRE scheme, 17:836ms and 23:62ms, respectively, for data sharing, indicates the efficiency of the schemes. The additional privacy provisioning step was responsible for the increased time in OTRE scheme. In addition to data sharing, consensus time, latency and throughput in KRE and OTRE schemes demonstrated their efficiency and suitability for healthcare data sharing applications.
Correlation of extracellular polymeric substances and microbial communities in biofilms with phosphate
Authors: Song Yanhui , Hua Jianlin , Ju Bao , Jiang Aili , Chen Lihong , Xing Ronglian 
Published: November 2022

Abstract
The characteristics and dynamic succession of biofilm on the surface of attached materials at different phosphate levels were studied. Phosphate showed significant effect on the formation of biofilm. The dry weight, dry weight without ash, chlorophyll-a, and extracellular polymeric substances (EPS) of biofilm all increased with the increase of phosphate level, and reached the peak value at 40 µg/L, then stabilized and slightly decreased with the increase of phosphate concentration. The proportion of polysaccharides in EPS of each layer in biofilm increased from outside to inside (from Soluble EPS to Loosely Bound EPS to Tightly Bound EPS), in contrast, the proportion of protein showed the opposite. EPS of various types in biofilms enhanced with the increase of phosphate level, the amounts of protein and polysaccharide in EPS showed similar trend. When the phosphate concentrations were lower than 40 µg/L, the ratio of protein to polysaccharide (PN/PS) increased with the increase of phosphate level, and decreased somewhat when the phosphate was higher than 50 µg/L. The proportion of bacteria in microbial population of biofilm decreased with the increasing of phosphate level and immersion time of the attached material, while the proportion of diatoms increased gradually. The study was helpful to understand the dialectical relationship between environmental factors and biological factors in the aquaculture environment, and provides a theoretical basis for exploring the mechanism of marine biofilm formation.
Alignment of human KAT2A (GCN5) Histone Acetyltransferase and SARS-CoV-2 Orf8 viral proteins
Authors: Lehrer Steven , Rheinstein Peter 
Published: November 2022

Abstract
Introduction: The SARS-CoV-2 virus has evolved to mimic an enzyme, KAT2A (lysine acetyltransferase 2A, GCN5), one of the histone proteins that package DNA in the cell nucleus. Gene transcription is deranged because of this mimicry, which reduces antiviral response. An amino acid sequence known as the ARKS motif in histone H3 is modified by KAT2A, which adds acetyl groups and encourages gene transcription. The Orf8 protein from the SARS-CoV-2 virus also has an ARKS motif. KAT2A interacts with Orf8 via ARKS, which modifies it and may cause KAT2A destruction. Using structures from RCSB Protein Data Bank, we examined another way that Orf8 may interfere with KAT2A.
Methods: We analyzed two RCSB Protein Data Bank molecules: 1) 1Z4R. Human GCN5 (KAT2A) Histone Acetyltransferase. 2) 7F5F. SARS-CoV-2 Orf8 S84 viral protein. The protein structures were superimposed and aligned on PYMOL v 2.5.0 with the Super command, which super aligns two protein selections.
Results: Pymol performed 5 cycles of calculations on 65 aligned atoms of Human KAT2A (GCN5 Histone Acetyltransferase) and SARS-CoV-2 Orf8 S84 viral proteins, with a final root mean square deviation of atomic positions (RMSD) of 0.975 Angstrom for 51 atoms. Lower values of RMSD indicate that alignment is validated with higher accuracy. RMSD values of 1 Angstrom or less indicate very good alignment.
Conclusion: the 51 amino acid alignment of Human KAT2A Histone Acetyltransferase and SARS-CoV-2 Orf8 S84 viral protein we identified suggests a significant effect of Orf8 on KAT2A. Orf8 may interfere with KAT2A gene transcription and disrupt host cell ability to regulate gene expression and respond to SARS-CoV-2 infection effectively. Since transcription and translation are upregulated in cancer cells, Orf8 could also be a cancer treatment.
Severe immediate hypersensitivity to gadolinium contrast agent after targeted treatment in a patient with alveolar soft part sarcoma: a case report
Authors: Yan Hongxia , Zhang Jianxin , Cai Xinfeng , Hao Zhiying , Guan Zhe 
Published: November 2022

Abstract
Background

Gadolinium-based contrast agents (GBCAs), benefiting from good tolerance and safety, become the priority contrast agents in magnetic resonance imaging (MRI). Serious hypersensitivity reactions caused by GBCAs are rare, but occur occasionally. The “immune surveillance” theory proposes that lowered immune function exists in patients with malignance, which decrease the occurrence of atopy. Natural immunosurveillance that enhanced by effective treatment of malignance may increase the risk of hypersensitivity.

Case presentation

A 29-year-old female patient suffered intensive pain with left leg mass was admitted in our hospital. She was diagnosed with alveolar soft part sarcoma by histopathological and revealed destruction of the left fibula and lung metastasis by computed tomography scan, and treated with anlotinib hydrochloride, a multi-targeted tyrosine kinase inhibitor. She underwent contrast-enhanced MRI twice using gadodiamide, without any discomfort. However, after four cycles of anlotinib hydrochloride, she developed severe immediate hypersensitivity due to gadopentetate dimeglumine-enhanced MRI for evaluating therapeutic regimen.

Conclusion

Severe immediate hypersensitivity might be occured by gadolinium contrast agent in patient with malignance after effective treatment. We explored the potential mechanism of GBCA-inducing hypersensitivity in detail, by especially focusing on the changes of immune environment. Furthermore, we propose new ideas for the safe use of GBCAs in patients with malignancies. However, to confirm the validity of these ideas, further research is required.
A suite of modular, all-synthetic suicide vectors for allelic exchange mutagenesis in multidrug resistant Acinetobacter strains
Authors: Pokhrel Alaska , Li Liping , Short Francesca L. , Paulsen Ian T. 
Published: November 2022

Abstract
Background: Acinetobacter baumannii is an opportunistic human pathogen that causes a variety of infections in immunosuppressed individuals and patients in intensive care units. The success of this pathogen in nosocomial settings can be directly attributed to its persistent nature and its ability to rapidly acquire multidrug resistance. It is now considered to be one of the top priority pathogens for development of novel therapeutic approaches. Several high-throughput techniques have been utilised to identify the genetic determinants contributing to the success of A. baumannii as a global pathogen. However, targeted gene-function studies remain challenging due to the lack of appropriate genetic tools.
Results: Here, we have constructed a series of all-synthetic allelic exchange vectors – pALFI1, pALFI2 and pALFI3 – with suitable selection markers for targeted genetic studies in highly drug resistant A. baumannii isolates. The vectors follow the Standard European Vector Architecture (SEVA) framework for easy replacement of components. This method allows for rapid plasmid construction with the mutant allele, efficient conjugational transfer using a diaminopimelic acid-dependent Escherichia coli donor strain, efficient positive selection using the suitable selection markers and finally, sucrose-dependent counter-selection to obtain double-crossovers.
Conclusions: We have used this method to create scar-less deletion mutants in three different strains of A. baumannii, which resulted in up to 75% deletion frequency of the targeted gene. We believe this method can be effectively used to perform genetic manipulation studies in multidrug resistant Gram-negative bacterial strains.
Item Analysis of Quality of Life Scale (QLICP-NA, V2.0) for Nasopharyngeal Carcinoma Based on Classical Test theory and Item Response Theory
Authors: Zhang Yani , Luo Tianxin , Chen Tianliang , Fang Yangchen , Wan Chonghua , Wu Jiayuan , Hu Liren , Liang Zhangye , Du Jinlin , Yang Zheng 
Published: November 2022

Abstract
Objective

To analyze the QLICP-NA (V2.0) items using classical test theory and item response theory.
Methods One hundred twenty-one nasopharyngeal carcinoma patients were investigated using QLICP-NA (V2.0). SPSS15.0 software was used to manage the data and Multilog 7.03 software was used for parameter analysis. IRT was used to calculate the discrimination, difficulty, and item information for each item in the scale.
Results The scale covered five domains: physical (PHD); psychological (PSD); social (SOD); general symptoms and side effects (SSD); and special module (SNA). PHD contains eight items (coded GPH1-8), PSD comprises nine items (coded GPS1-9), SOD covers eight items (coded GSO1-8), SSD includes seven items (coded GSS1-7), and SNA covers eleven items (coded SNA1-11). The correlation coefficient between SNA1 and SNA8 was < 0.4, and the correlation was poor. GPS4, GOS4, SNA1, and SNA3 had low correlation with the field. The five items were deleted. SNA4 and SNA8 discrimination was poor, thus deletion was recommended. SNA2 and SNA10 discrimination was not good, thus SNA2 and SNA10 were modified and retained. The results of item information showed that the information content of the specific module, SNA1-11, was poor, but because deleting too many items had a great impact on the total table and deleting two items (SNA6 and SNA10) with < 0.2 information, 34 items were finally reserved.
Conclusion The analysis of scale items can be evaluated from different angles and applied comprehensively.
Current Status and Prospects of Diabetes Mellitus Induced Erectile Dysfunction: A Bibliometric and Visualization Study
Authors: Liu Baoxing , Zhang Lei , Bao Binghao , Guo Jianqiang , Qin Zhongjian , Huang Haonan , Chen Lu 
Published: November 2022

Abstract
The prevalence of diabetes mellitus-induced erectile dysfunction (DMED) has recently increased, which has prompted numerous DMED studies. Here, we conduct a bibliometric analysis of relevant literature in the field of DMED and to discuss the research hotspots and future development directions. The Web of Science Core Collection database was searched for literature on DMED, and literature characterization was performed using VOS viewer 1.6.18 and CiteSpace 5.8 R3 software. A total of 804 articles concerning DMED were included in this study. The Journal of Sexual Medicine issued the most documents. The United States and China were in the leading position in the field of DMED research. Ryu JK were the authors with the highest number of documents issued while Bivalacqua TJ was the author with the most co-citated. The keywords analysis shows that the main research hotspots in the field of DMED were mechanism discussions and disease treatment and management. Global research on DMED is expected to increase further. The investigation of the mechanism of DMED and the exploration of new therapeutic means and targets are the focus of future research.
Choline deficiency and choline metabolism disorders can lead to anxiety-like behaviors induced by DSS in IBD mice
Authors: Xu Maosheng , Zhang Fan , Guo Lingnan , Shi Jingjing , Jiang Hao , Zhou Feini , Zhou Yanlin , Lv Bin 
Published: November 2022

Abstract
Anxiety and depression caused by inflammatory bowel diseases (IBD) negatively affect the mental health of patients. Emerging studies have demonstrated that the gut-brain axis mediates IBD-induced mood disorders, but the underlying mechanisms of these findings remain unknown. This study unveiled promising evidence that choline dysfunction may be a cause of IBD induced mood disorders. Analysing Dextran Sulfate Sodium Salt (DSS)-induced IBD mice model with transcriptomics and metabolomics technology, it was discovered that mRNA responsible for acetylcholine synthesis and secretion were increased and the phosphatidylcholine (PC) content were decreased in prefrontal cortex (PFC) of IBD mice compared to the Control. Fecal Metagenomics showed that the microbiome and lipid metabolism were also abnormal in IBD mice. Since both acetylcholine and PC are choline metabolites, we inferred that the IBD mice may suffer from choline deficiency and choline metabolism disorder. Following supplementation of CDP-choline, experimental subjects showed improvements through decreased anxiety-like behaviors, reduced PC degradation and increased acetylcholine synthesis in the PFC. In addition, CDP-choline treatment was shown to restore the gut microbiome and lipid metabolism disorders characteristic of DSS treatment. This study provides compelling evidence to suggest that choline metabolism plays a key role in the development and treatment of mood disorders in IBD patients, and choline and its metabolites may play a key role in maintaining the stability of the gut-brain axis.
A novel somatic mutation in POLE exonuclease domain associated with ultra-mutational signature and MMR deficiency in endometrial cancer: a case report
Authors: Cui Jiantao , Chen Xiuying , Zhai Qian , Chen Na , Li Xiaodan , Zhang Yuli , Wang Hui , Bian Xin , Gao Na , Chen Deyi , Chen Zhihong , Zhang Shibiao , Chen Yan 
Published: November 2022

Abstract
Background: Defect in proofreading exonuclease activity of polymerases epsilon and delta (Pols ε and δ) leads to mutagenesis and genomic instability and has been described in several cancer types. Somatic POLE exonuclease domain mutations (EDMs) have been reported in 7-12% endometrial cancers (ECs) and defined a subgroup of endometrial cancers with ultrahigh somatic mutation frequencies, high tumor infiltrated lymphocytes and favorable outcomes.
Case presentation: Herein, we presented a novel somatic mutation in POLE exonuclease domain associated with ultra-mutational signature and MMR deficiency in endometrial cancer. A novel POLE EDM (p.T278K) was found by a 11-gene NGS panel. The MSS status detected by the MSI test was inconsistent with the dMMR status by IHC. The loss of MSH6 expression in the tumor could be interpreted by the two nonsense mutations (p.E1234* and p.E1322*) of the MSH6 gene which may lead to truncated proteins. The T278K mutation was pathogenic identified by a 602-gene NGS panel with 27.3% of C>A substitution, 0.6% of indels, 0.6% of C>G substitution and a high TMB of 203.8 mut/Mb.
Conclusions: We report an endometrial cancer patient harbored a novel somatic POLE T278K mutation. This mutation was a novel pathogenic POLE EDM should be considered as “POLE (ultramutated)” in clinical practice for the molecular classification of EC.