bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mnk1/2 kinases regulate memory and autism-related behaviours via Syngap1
Authors: Chalkiadaki Kleanthi , Hooshmandi Mehdi , Lach Gilliard , Statoulla Elpida , Simbriger Konstanze , Amorim Ines S , Kouloulia Stella , Zafeiri Maria , Pothos Panagiotis , Bonneil Éric , Gantois Ilse , Popic Jelena , Kim Sung-Hoon , Wong Calvin , Cao Ruifeng , Komiyama Noboru H , Atlasi Yaser , Jafarnejad Seyed Mehdi , Khoutorsky Arkady , Gkogkas Christos G 
Published: October 2022

Abstract
MAPK interacting protein kinases 1 and 2 (Mnk1/2) regulate a plethora of functions, presumably via phosphorylation of their best characterized substrate, eukaryotic translation initiation factor 4E (eIF4E) on Ser209. Here, we show that, whereas deletion of Mnk1/2 (Mnk double knockout) impairs synaptic plasticity and memory in mice, ablation of phospho-eIF4E (Ser209) does not affect these processes, suggesting that Mnk1/2 possess additional downstream effectors in the brain. Translational profiling revealed only a small overlap between the Mnk1/2- and phospho-eIF4E(Ser209)-regulated translatome. We identified the synaptic Ras GTPase activating protein 1 (Syngap1), encoded by a syndromic autism gene, as a downstream target of Mnk1 because Syngap1 immunoprecipitated with Mnk1 and showed reduced phosphorylation (S788) in Mnk double knockout mice. Knockdown of Syngap1 reversed memory deficits in Mnk double knockout mice and pharmacological inhibition of Mnks rescued autism-related phenotypes in Syngap1+/− mice. Thus, Syngap1 is a downstream effector of Mnk1, and the Mnks–Syngap1 axis regulates memory formation and autism-related behaviours.
Squid Game and the imagining of Afro-Asian connections through Black Twitter memescapes
Authors: Love Ta’Les , Kim Youngrim 
Published: October 2022

Abstract
The release of Netflix’s Squid Game facilitated a viral pop culture moment, as the show’s critique of capitalism and structural inequality resonated with global audiences. Memes about the series were frequently shared across social media, as users interpolated themselves into the Squid Game universe. We argue that the memes produced by Black Twitter users are a form of meme play that can be read as a significant moment in the shared cultural and activist lineage of Black and Korean communities. Although racial tension is often at the center of the relationship between these two marginalized groups, we suggest that Black Twitter users’ interpolation of themselves into a distinctly Korean cultural product provides us with a chance to imagine how interracial solidarities can be visualized. This is significant in the age of the Black Lives Matter and Stop Asian Hate Movements, which are supported by members of both communities.
Higher Levels of Cerebrospinal Fluid and Plasma Neurofilament Light in Human Immunodeficiency Virus-Associated Distal Sensory Polyneuropathy
Authors: Ellis Ronald J , Chenna Ahmed , Lie Yolanda , Curanovic Dusica , Winslow John , Tang Bin , Marra Christina M , Rubin Leah H , Clifford David B , McCutchan J Allen , Gelman Benjamin B , Robinson-Papp Jessica , Petropoulos Christos J , Letendre Scott L 
Published: October 2022

Abstract

Background
Neurofilament light (NFL) chain concentrations, reflecting axonal damage, are seen in several polyneuropathies but have not been studied in human immunodeficiency virus (HIV) distal sensory polyneuropathy (DSP). We evaluated NFL in cerebrospinal fluid (CSF) and plasma in relation to DSP in people with HIV (PWH) from 2 independent cohorts and in people without HIV (PWoH).

Methods
Cohort 1 consisted of PWH from the CHARTER Study. Cohort 2 consisted of PWH and PWoH from the HIV Neurobehavioral Research Center (HNRC). We evaluated DSP signs and symptoms in both cohorts. Immunoassays measured NFL in CSF for all and for plasma as well in Cohort 2.

Results
Cohort 1 consisted of 111 PWH, mean ± SD age 56.8 ± 8.32 years, 15.3% female, 38.7% Black, 49.6% White, current CD4+ T-cells (median, interquartile range [IQR]) 532/µL (295, 785), 83.5% with plasma HIV RNA ≤50 copies/mL. Cohort 2 consisted of 233 PWH of similar demographics to PWH in Cohort 1 but also 51 PWoH, together age 58.4 ± 6.68 years, 41.2% female, 18.0% Black, Hispanic, non-Hispanic White 52.0%, 6.00% White. In both cohorts of PWH, CSF and plasma NFL were significantly higher in both PWH with DSP signs. Findings were similar, albeit not significant, for PWoH. The observed relationships were not explained by confounds.

Conclusions
Both plasma and CSF NFL were elevated in PWH and PWoH with DSP. The convergence of our findings with others demonstrates that NFL is a reliable biomarker reflecting peripheral nerve injury. Biomarkers such as NFL might provide, validate, and optimize clinical trials of neuroregenerative strategies in HIV DSP.
Effectiveness of Evusheld in Immunocompromised Patients: Propensity Score–Matched Analysis
Authors: Najjar-Debbiny Ronza , Gronich Naomi , Weber Gabriel , Stein Nili , Saliba Walid 
Published: October 2022

AbstractBackgroundTixagevimab and cilgavimab, a combined monoclonal antibody (Evusheld), was granted emergency use authorization for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) preexposure prophylaxis in individuals with immunocompromising conditions. In this study we used population-based real-world data to evaluate the effectiveness of Evusheld in immunocompromised patients.MethodsUsing the computerized database of the largest healthcare provider in Israel, we identified all adult immunocompromised patients who were eligible to receive Evusheld (150 mg tixagevimab and 150 mg cilgavimab) on 15 February 2022. Patients with a documentation of a prior SARS-CoV-2 infection were excluded. A total of 703 patients who received Evusheld were propensity score matched, using a ratio of 1:4, with 2812 patients who had not received Evusheld (control group). Patients were followed through 30 June 2022 for up to 90 days for the first documentation of SARS-CoV-2 infection and coronavirus disease 2019 (COVID-19)–related hospitalization.ResultsOverall, 72 patients in the Evusheld group and 377 patients in the control group had SARS-CoV-2 infection, reflecting an incidence rate of 4.18 and 5.64 per 100 person-months, respectively. The hazard ratios were 0.75 (95% confidence interval [CI]: .58–.96) for SARS-CoV-2 infection and 0.41 (95% CI: .19–.89) for COVID-19–related hospitalization in the Evusheld group compared to the control group. The magnitude of relative risk reduction of each outcome was greater in nonobese patients (P for interaction = .020 and .045, respectively).ConclusionsThis study suggests that Evusheld is effective in reducing the risk of SARS-CoV-2 infection and COVID-19 hospitalization in immunocompromised patients. The effectiveness of this dose appears to be greater in nonobese patients.
Microbial Volatiles as Diagnostic Biomarkers of Bacterial Lung Infection in Mechanically Ventilated Patients
Authors: Ahmed Waqar M , Fenn Dominic , White Iain R , Dixon Breanna , Nijsen Tamara M E , Knobel Hugo H , Brinkman Paul , Van Oort Pouline M P , Schultz Marcus J , Dark Paul , Goodacre Royston , Felton Timothy , Bos Lieuwe D J , Fowler Stephen J ,   for the BreathDx Consortium, Ahmed Waqar M , Raventos Antonio Artigas , Bannard-Smith Jonathan , Bos Lieuwe D J , Camprubi Marta , Coelho Luis , Dark Paul , Davie Alan , Diaz Emili , Goma Gemma , Felton Timothy , Fowler Stephen J , Goodacre Royston , Johnson Craig , Knobel Hugo , Lawal Oluwasola , Leopold Jan-Hendrik , Martin-Loeches Ignacio , Nijsen Tamara M E , van Oort Pouline M P , Povoa Pedro , Rattray Nicholas J W , Rijnders Guus , Schultz Marcus J , Steenwelle Ruud , Sterk Peter J , Valles Jordi , Verhoeckx Fred , Vink Anton , Weda Hans , White Iain R , Winters Tineke , Zakharkina Tetyana 
Published: October 2022

AbstractBackgroundEarly and accurate recognition of respiratory pathogens is crucial to prevent increased risk of mortality in critically ill patients. Microbial-derived volatile organic compounds (mVOCs) in exhaled breath could be used as noninvasive biomarkers of infection to support clinical diagnosis.MethodsIn this study, we investigated the diagnostic potential of in vitro–confirmed mVOCs in the exhaled breath of patients under mechanical ventilation from the BreathDx study. Samples were analyzed by thermal desorption–gas chromatography–mass spectrometry.ResultsPathogens from bronchoalveolar lavage (BAL) cultures were identified in 45 of 89 patients and Staphylococcus aureus was the most commonly identified pathogen (n = 15). Of 19 mVOCs detected in the in vitro culture headspace of 4 common respiratory pathogens (S. aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli), 14 were found in exhaled breath samples. Higher concentrations of 2 mVOCs were found in the exhaled breath of patients infected with S. aureus compared to those without (3-methylbutanal: P &lt; .01, area under the receiver operating characteristic curve [AUROC] = 0.81–0.87; and 3-methylbutanoic acid: P = .01, AUROC = 0.79–0.80). In addition, bacteria identified from BAL cultures that are known to metabolize tryptophan (E. coli, Klebsiella oxytoca, and Haemophilus influenzae) were grouped and found to produce higher concentrations of indole compared to breath samples with culture-negative (P = .034) and other pathogen-positive (P = .049) samples.ConclusionsThis study demonstrates the capability of using mVOCs to detect the presence of specific pathogen groups with potential to support clinical diagnosis. Although not all mVOCs were found in patient samples within this small pilot study, further targeted and qualitative investigation is warranted using multicenter clinical studies.
New Perspectives for Prosthetic Valve Endocarditis: Impact of Molecular Imaging by FISHseq Diagnostics
Authors: Hajduczenia Maria M , Klefisch Frank R , Hopf Alexander G M , Grubitzsch Herko , Stegemann Miriam S , Pfäfflin Frieder , Puhlmann Birgit , Ocken Michele , Kretzler Lucie , von Schöning Dinah , Falk Volkmar , Moter Annette , Kikhney Judith 
Published: October 2022

AbstractBackgroundThe microbial etiology of prosthetic valve infective endocarditis (PVE) can be difficult to identify. Our aim was to investigate the benefit of molecular imaging technique fluorescence in situ hybridization (FISH) combined with 16S rRNA-gene polymerase chain reaction (PCR) and sequencing (FISHseq) for the analysis of infected prosthetic heart valves.MethodsWe retrospectively evaluated the diagnostic outcome of 113 prosthetic valves from 105 patients with suspected PVE, treated in 2003–2013 in the Department of Cardiac Surgery, Charité University Medicine Berlin. Each prosthetic valve underwent cultural diagnostics and was routinely examined by FISH combined with 16S rRNA gene PCR and sequencing. We compared classical microbiological culture outcomes (blood and valve cultures) with FISHseq results and evaluated the diagnostic impact of the molecular imaging technique.ResultsConventional microbiological diagnostic alone turned out to be insufficient, as 67% of preoperative blood cultures were noninformative (negative, inconclusive, or not obtained) and 67% of valve cultures remained negative. FISHseq improved the conventional cultural diagnostic methods in PVE in 30% of the cases and increased diagnostic accuracy. Of the valve culture–negative PVE cases, FISHseq succeeded in identifying the causative pathogen in 35%.ConclusionsFISHseq improves PVE diagnostics, complementing conventional cultural methods. In addition to species identification, FISH provides information about the severity of PVE and state of the pathogens (eg, stage of biofilm formation, activity, and localization on and within the prosthetic material). As a molecular imaging technique, FISHseq enables the unambiguous discrimination of skin flora as contaminant or infectious agent.
Modularity of the ABCD random graph model with community structure
Authors: Kamiński Bogumił , Pankratz Bartosz , Prałat Paweł , Théberge François 
Published: October 2022

Abstract
The Artificial Benchmark for Community Detection (ABCD) graph is a random graph model with community structure and power-law distribution for both degrees and community sizes. The model generates graphs with similar properties as the well-known LFR (Lancichinetti, Fortunato, Radicchi) one, and its main parameter $\xi$ can be tuned to mimic its counterpart in the LFR model, the mixing parameter $\mu$. In this article, we investigate various theoretical asymptotic properties of the ABCD model. In particular, we analyse the modularity function, arguably, the most important graph property of networks in the context of community detection. Indeed, the modularity function is often used to measure the presence of community structure in networks. It is also used as a quality function in many community detection algorithms, including the widely used Louvain algorithm.
A Phase IB/IIA Study of Allogeneic, Bone Marrow-derived, Mesenchymal Stem Cells for the Treatment of Refractory Ileal-anal Anastomosis and Peripouch Fistulas in the Setting of Crohn’s Disease of the Pouch
Authors: Lightner Amy L , Reese Jane , Ream Justin , Nachand Douglas , Jia Xue , Pineiro Ana Otero , Dadgar Neda , Steele Scott , Hull Tracy 
Published: October 2022

Abstract

Background and Aims
Mesenchymal stem cells [MSCs] have been used for the treatment of perianal Crohn’s fistulising disease by direction injection. No studies to date have included patients with an ileal pouch-anal anastomosis [IPAA] in situ.

Methods
A phase IB/IIA, randomised, control trial of bone marrow-derived, allogeneic MSCs via direct injection to treat adult patients with a peripouch fistula[s] was conducted; 75 million MSCs were administered with a 22 G needle, with repeat injection at 3 months if complete clinical and radiographic healing was not achieved. Adverse and serious adverse events at post-procedure Day 1, Week 2, Week 6, Month 3, Month 6, and Month 12 were assessed. Clinical healing, radiographic healing per pelvic magnetic resonance imaging [MRI], and patient-reported outcomes were assessed at the same time points.

Results
A total of 22 patients were enrolled and treated; 16 were treated and six were controls. There were no adverse or serious adverse events related to MSC therapy. At 6 months, 31% of the treatment group and 20% of the control had complete clinical and radiographic healing. When stratifying the treatment group into perianal [n = 7] and ano-vaginal [n = 8] fistulas, 6-month healing in the treatment groups was 57% and 0%, respectively. The perianal Crohn’s disease activity index [PCDAI], Wexner incontinence score, and van Assche score all significantly decreased in treatment patients at 6 months; only the PCDAI decreased in the control group.

Conclusion
Bone marrow-derived, allogeneic MSCs offer a safe and effective alternative treatment approach for peripouch fistulas in the setting of a Crohn’s like phenotype of the pouch [ClinicalTrials.gov Identifier: NCT04519684.]
Discrimination Abilities and Parasitism Success of Pupal Parasitoids Towards Spotted-Wing Drosophila Pupae Previously Parasitized by the Larval Parasitoid Ganaspis brasiliensis (Hymenoptera: Figitidae)
Authors: Hougardy Evelyne , Hogg Brian N , Wang Xingeng , Daane Kent M 
Published: October 2022

Abstract
Pachycrepoideus vindemiae (Rondani) (Hymenoptera: Pteromalidae) and Trichopria drosophilae (Perkins) (Hymenoptera: Diapriidae) are two cosmopolitan and generalist pupal parasitoids that are among a few of the resident parasitoids in North America capable of attacking Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), an invasive pest of small and soft fruit crops worldwide. Ganaspis brasiliensis (Ihering) is a specialist larval parasitoid of D. suzukii that was recently approved for biological control introduction against D. suzukii in the USA. As a solitary koinobiont species, G. brasiliensis oviposits in the host larva but emerges as an adult from the host puparium. This study investigated the discrimination ability and parasitism success by the pupal parasitoids towards D. suzukii pupae previously parasitized by G. brasiliensis, to examine whether interactions with resident parasitoids will affect G. brasiliensis after it is released in the USA. We found preliminary evidence that neither pupal parasitoid could discriminate towards D. suzukii pupae parasitized by early instars of G. brasiliensis. Pachycrepoideus vindemiae was able to successfully develop on D. suzukii pupae containing all preimaginal stages of G. brasiliensis, although parasitism success was significantly higher on those bearing later rather than early stages of G. brasiliensis. Trichopria drosophilae was only able to successfully develop on D. suzukii puparia containing early instars of G. brasiliensis. These results suggest that D. suzukii parasitized by the larval parasitoid could be subsequently attacked by the pupal parasitoids, possibly affecting the success of G. brasiliensis releases.
New England in the Royalist Imagination, 1637–89
Authors: Fullerton Samuel 
Published: October 2022

Abstract
This essay examines representations of New England puritanism in royalist print and manuscript polemic during the English Revolution of 1642–1660 and beyond. Royalist attitudes toward the North American godly colonies have been largely overlooked by modern scholars, who continue to privilege New England’s significance in intra-parliamentarian religious debates over its impact on the events of the civil war itself. As a result, they have neglected a substantial canon of hostile anti-puritan royalist literature that identified colonial puritanism, along with other malevolent international godly influences, as a horrifying example of the future awaiting old England if Charles I’s parliamentarian enemies were to triumph. As a pre-eminent puritan sanctuary from which some godly rebels had been recruited to perpetuate the civil war in Britain and to which domestic parliamentarians later allegedly intended to flee once their uprising had succeeded, New England thus occupied an important place in the royalist political imagination. Although royalist attitudes toward the American puritan colonies waxed and waned in significance during the revolutionary decades, and while New Englanders themselves may not have paid considerable attention to hostile royalist commentary, interested English readers and domestic parliamentarian polemicists alike paid a great deal of attention to the anti-colonial thrust of mid-century cavalier print. Moreover, certain aspects of royalist anti-New England rhetoric lived on to provide later anti-puritan commentators with a convenient explanation for the ongoing hostility of colonial puritans toward Stuart rule for decades following the Restoration.
Defining the optimal revascularization strategy during protected high-risk procedures with Impella
Authors: Sinning Jan-Malte , Al-Rashid Fadi , Ibrahim Karim , Aurigemma Cristina , Chieffo Alaide 
Published: October 2022

Abstract
Complete revascularization (CR) in patients with multi-vessel disease improves outcomes. The use of percutaneous left-ventricular assist devices, such as the Impella heart pump, is useful to minimize the risk of haemodynamic compromise in complex higher risk and clinically indicated patients. The recently published data from the PROTECT III trial suggest more CR during Impella-protected percutaneous coronary intervention with more extensive lesion preparation and treatment, resulting in the reduced need for repeat revascularization. To achieve CR and improve survival, procedural guidance by intravascular imaging, extensive lesion preparation, debulking with atherectomy devices, advanced chronic total occlusion revascularization techniques, and post-interventional treatment with modern anti-platelet medication are essential.
Pyp25α is required for male gametocyte exflagellation
Authors: Zhang Chao , Li Deyi , Meng Zhirong , Zhou Jianwei , Min Zhenxiao , Deng Shengqun , Shen Jijia , Liu Miao 
Published: October 2022

Abstract
Malaria, a mosquito-borne infectious disease, is caused by the unicellular apicomplexan protozoa of the genus Plasmodium. For malaria parasite transmission, the essential sexual stage includes production of gametocytes through gametocytogenesis in vertebrate hosts and formation of gametes from gametocytes through gametogenesis in mosquito vectors. Whereas each female gametocyte forms a single immotile macrogamete, a male gametocyte produces eight flagella-like microgametes in a process called exflagellation. We identified a conserved protein named as Py05543 (Pyp25α), required for male gametocyte exflagellation in Plasmodium yoelii, which is the ortholog of PFL1770c (PF3D7_1236600). Interestingly, PF3D7_1236600 was previously phenotypically screened to be gametocyte-essential genes during gametocytogenesis of Plasmodium falciparum, using piggyBac transposon-mediated insertional mutagenesis. In this study, using CRISPR/Cas9-mediated genome editing, the Pyp25α¯ (KO) parasite line was successfully established. We found that the KO parasites proliferated asexually in mouse blood normally. In addition, compared with that of the parental parasites, the KO parasites displayed similar levels of gametocytes formation. Unexpectedly, the KO parasites showed considerable deficiency in exflagellation of male gametes, by observing exflagellation centre formation. Taken together, our data suggested that Pyp25α gene, the ortholog of PF3D7_1236600, was nonessential for the growth of asexual parasites, required for male gametocyte exflagellation in P. yoelii.
Genetic variants in promoter region of TFR2 is associated with the risk of non-alcoholic fatty liver disease in a Chinese Han population: a case–control study
Authors: Pan Xinting , Peng Hewei , Zhang Junchao , Wu Yunli , Hu Zhijian , Peng Xian-E 
Published: October 2022

Abstract

Background
Iron overload is frequently observed in non-alcoholic fatty liver disease (NAFLD). Transferrin receptor 2 (TFR2) is an important key factor in iron regulation. We aimed to investigate whether TFR2 single nucleotide polymorphisms (SNPs) contribute to susceptibility to NAFLD in a Chinese Han population.

Methods
Five tag SNPs (rs10247962, rs4434553, rs2075672, rs1052897, and rs3757859) in the TFR2 gene were selected and genotyped in a case–control study on participants who visited two affiliated hospitals of Fujian Medical University between June 2011 and August 2017. Propensity score matching and inverse probability of treatment weighting analyses were used to verify the risk associated with TFR2 SNPs.

Results
Logistic regression analyses suggested that subjects with the rs4434553 GA or GG genotype had a lower risk of NAFLD than those carrying the AA genotype (odds ratio = 0.630, 95% confidence interval = 0.504–0.788). Moreover, the rs4434553 GA or GG genotype was negatively correlated with body mass index, hepatic steatosis index, and serum ferritin (b = −0.363, P = 0.008; b = −1.040, P = 0.009; b = −35.258, P = 0.015, respectively), and positively associated with serum hepcidin level (b = 35.308, P &lt; 0.001). Moreover, rs10247962 and rs1052897 had multiplicative interactions with age in relation to the risk of NAFLD (P for interactions, 0.041 and 0.034, respectively). The cumulative effects of the rs10247962, rs1052897, and rs4434553 SNPs were positively associated with the risk of NAFLD (adjusted Ptrend = 0.012).

Conclusions
In this Chinese Han population, the rs4434553 polymorphism in TFR2 may be an independent influencing factor associated with the susceptibility to NAFLD. The ageing effect on the development of NAFLD may be inhibited by SNPs rs10247962 and rs1052897.