bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advances in digital CBT: where are we now, and where next?
Authors: Thew Graham R. , Rozental Alexander , Hadjistavropoulos Heather D. 
Published: October 2022

Abstract
Digital CBT refers to the use of digital tools, platforms or devices to deliver or enhance cognitive behavioural therapy assessment, formulation, treatment, training and supervision. The ‘Advances in Digital CBT’ special issue aimed to document examples of innovative digital CBT practice in this rapidly developing field. In this paper, we have briefly summarised and synthesised the advances demonstrated in this group of articles. These include developments in our understanding of mental health apps, the use of digital tools as an adjunct to therapy, the effectiveness of remotely delivered CBT in routine clinical practice, our understanding of user experiences and involvement, and in digital CBT research methods. We consider the extent of current knowledge in these areas and identify where gaps in evidence lie and how the field could be taken forward to address these. Lastly, we reflect on the broader digital CBT picture and offer our suggestions of six key directions for future research: using robust study designs to evaluate and optimise digital tools; translating and culturally adapting digital tools and practices; understanding and addressing digital exclusion; exploring, reporting and addressing possible negative effects; improving user involvement in design and evaluation; and addressing the implementation gap for digital tools. We suggest that further advances in these areas would be of particular benefit to the digital CBT field.

Key learning aims

(1)
To gain an overview of the articles in the special issue and an understanding of the advances in digital CBT they represent.

(2)
To understand how the advances suggested by the present studies could be taken forward and extended.

(3)
To consider key future directions for further advances in digital CBT.
Geysers, Boiling Groundwater and Tectonics: The 3D Subsurface Resistive Structure of the Haukadalur Hydrothermal Field, Iceland
Authors: Lupi Matteo , Collignon Marine , Fischanger Federico , Carrier Aurore , Trippanera Daniele , Pioli Laura 
Published: October 2022

AbstractGeysers are among the most fascinating geological features on Earth. Yet, little is still known about their hydrogeological structure at depth. To shed light on the spatial relationships between the vertical conduits and the aquifers feeding them, we conducted a 3D geoelectrical campaign in the Haukadalur hydrothermal field, Iceland. We deployed 24 Iris Fullwavers across the hydrothermal field and inverted resistivity and chargeability measurements. Additionally, we measured temperature variations inside Strokkur and Great Geysir geysers showing temperature fluctuations pointing out the oscillatory behavior that characterizes the geysering cycle of the geysers. By combining a semi‐quantitative temperature distribution of the thermal springs across the hydrothermal field with the inversion of the geoelectrical data, we highlight the control that extensional tectonics have on the distribution of fluids across the hydrothermal field. We also point out the occurrence of a common deep groundwater reservoir feeding the hydrothermal centers. Induced polarization data show that the geysers are fed by sub‐vertical water‐filled fracture zones. The geysers are found at the margins of highly resistive regions where we speculate boiling groundwater and vapor is found. Our proposed model suggests that local waters feeding the main groundwater reservoir downwell from the nearby region and then convect upwards, phase transitioning into vapor at about 200 m depth. From here, fluids flow toward the surface through pipes cutting a highly pressurized and hot system. This study shows to the best of our knowledge the first full 3D tomographic image of a hydrothermal field hosting geysers.
Joint Interpretation of Marine Self‐Potential and Transient Electromagnetic Survey for Seafloor Massive Sulfide (SMS) Deposits: Application at TAG Hydrothermal Mound, Mid‐Atlantic Ridge
Authors: Su Z. , Tao C. , Zhu Z. , Revil A. , Shen J. , Nie Z. , Li Q. , Deng X. , Zhou J. , Liu L. 
Published: October 2022

AbstractThe self‐potential (SP) method is expected to play an important role in the exploration of seafloor massive sulfide (SMS) resources. Since the redox potential changes with depth below the seafloor, SMS deposits are the source of an electrical (source) current density, generating in turn a recordable electrical field. This electrical field can be remotely measured at and above the seafloor. By integrating this electrical field, we obtained a so‐called SP profile or map. The electrical conductivity distribution of SMS deposits is an important ingredient in the inversion of these SP data in order to distinguish between causative primary sources (i.e., associated with the SMS deposits themselves) and secondary current sources associated with conductivity contrasts below the seafloor. Such ingredient is therefore necessary to properly localize SMS deposits and eliminating ghosts associated with high conductivity contrasts. The conductivity structure of the seafloor is however unknown in most SP surveys. This study presents the first joint interpretation of transient electromagnetic and SP surveys. The field data were acquired at the Trans‐Atlantic Geotraverse (TAG) hydrothermal mound. The conductivity structure of TAG mound was obtained by inverting transient electromagnetic data. This electrical conductivity distribution is then used to invert the SP data. The obtained (primary) source current distribution delineates the geometry of the SMS deposits. The deposits are located in the center of the mound as well as at its edges, in a way that is consistent with water column data. Combining the two geophysical methods improves our ability to better decipher the fine geometry of SMS deposits below the seafloor.
SF3B1 mutated MDS: Blast count, genetic co-abnormalities and their impact on classification and prognosis
Authors: Huber Sandra , Haferlach Torsten , Meggendorfer Manja , Hutter Stephan , Hoermann Gregor , Baer Constance , Kern Wolfgang , Haferlach Claudia 
Published: October 2022

AbstractRecently, MDS with mutated SF3B1 and blast count <5% was proposed as distinct entity with favorable prognosis by the international working group for the prognosis of MDS (IWG-PM), the 5th edition of the WHO classification and the International Consensus Classification. To further characterize this entity with respect to the genomic landscape, AML transformation rate and clinical outcome, we analyzed 734 MDS patients by whole genome sequencing. SF3B1 mutations were identified in 31% (n = 231), most frequently accompanied by TET2 mutations (29%). 144/231 (62%) SF3B1mut samples fulfilled entity criteria proposed by IWG-PM (SF3B1ent). These cases were associated with longer survival, lower AML transformation rate, normal karyotypes and harbored less accompanying mutations compared to SF3B1mut samples not falling into the proposed SF3B1 entity (SF3B1nent). Of SF3B1mut cases 7% (15/231; SF3B1ent: 3/144 [2%]; SF3B1nent: 12/87 [14%]) progressed to AML compared to 15% SF3B1 wild-type patients (75/503). Of these 15 SF3B1mut cases, 10 (67%) showed RUNX1 mutations at MDS or AML stage. Multivariate analysis revealed that del(5q) and RUNX1 mutations were independent negative prognostic factors for overall survival, while blast count >5% was not. In conclusion, SF3B1mut MDS has a favorable prognosis independent of blast count if karyotype and RUNX1 mutations are considered.
Genome-centric investigation of bile acid metabolizing microbiota of dairy cows and associated diet-induced functional implications
Authors: Lin Limei , Lai Zheng , Yang Huisheng , Zhang Jiyou , Qi Weibiao , Xie Fei , Mao Shengyong 
Published: October 2022

Abstract
Although the importance of bile acid (BA)-related microbial strains and enzymes is increasingly recognized for monogastric animals, a lack of knowledge about BA metabolism in dairy cows limits functional applications aimed at the targeted modulation of microbe–host interactions for animal production and health. In the present study, 108 content samples from six intestinal regions of dairy cows were used for shotgun metagenomic sequencing. Overall, 372 high-quality metagenome-assembled genomes (MAGs) were involved in BA deconjugation, oxidation, and dehydroxylation pathways. Furthermore, the BA-metabolizing microbiome predominately occurred in the large intestine, resulting in the accumulation of secondary unconjugated BAs. Comparative genomic analysis revealed that the bile salt hydrolase (BSH)-carrying microbial populations managed with the selective environment of the dairy cow intestine by adopting numerous host mucin glycan-degrading abilities. A sequence similarity network analysis classified 439 BSH homologs into 12 clusters and identified different clusters with diverse evolution, taxonomy, signal peptides, and ecological niches. Our omics data further revealed that the strains of Firmicutes bacterium CAG-110 processed the increased abundance of BSHs from Cluster 1, coinciding with the changes in the colon cholic acid concentration after grain introduction, and were intricately related to intestinal inflammation. This study is the first to use a genome-centric approach and whole intestine-targeted metabolomics to reveal microbial BA metabolism and its diet-induced functional implications in dairy cows. These findings provide insight into the manipulation of intestinal microorganisms for improving host health.
Light-independent anaerobic microbial oxidation of manganese driven by an electrosyntrophic coculture
Authors: Huang Lingyan , Liu Xing , Rensing Christopher , Yuan Yong , Zhou Shungui , Nealson Kenneth H 
Published: October 2022

Abstract
Anaerobic microbial manganese oxidation (AMMO) has been considered an ancient biological metabolism for Mn element cycling on Archaean Earth before the presence of oxygen. A light-dependent AMMO was recently observed under strictly anoxic conditions, providing a new proxy for the interpretation of the evolution of oxygenic photosynthesis. However, the feasibility of biotic Mn(II) oxidation in dark geological habitats that must have been abundant remains unknown. Therefore, we discovered that it would be possible to achieve AMMO in a light-independent electrosyntrophic coculture between Rhodopseudomonas palustris and Geobacter metallireducens. Transmission electron microscopy analysis revealed insoluble particle formation in the coculture with Mn(II) addition. X-ray diffraction and X-ray photoelectron spectroscopy analysis verified that these particles were a mixture of MnO2 and Mn3O4. The absence of Mn oxides in either of the monocultures indicated that the Mn(II)-oxidizing activity was induced via electrosyntrophic interactions. Radical quenching and isotopic experiments demonstrated that hydroxyl radicals (•OH) produced from H2O dissociation by R. palustris in the coculture contributed to Mn(II) oxidation. All these findings suggest a new, symbiosis-dependent and light-independent AMMO route, with potential importance to the evolution of oxygenic photosynthesis and the biogeochemical cycling of manganese on Archaean and modern Earth.
Serplulimab, a novel anti-PD-1 antibody, in patients with microsatellite instability-high solid tumours: an open-label, single-arm, multicentre, phase II trial
Authors: Qin Shukui , Li Jin , Zhong Haijun , Jin Chuan , Chen Lili , Yuan Xianglin , Fan Qingxia , Chen Kehe , Cao Peiguo , Xiao Jianjun , Jiang Da , Zhang Tao , Zhang Hongyu , Wang Xicheng , Wang Wei , Han Lin , Wang Qingyu , Zhu Jun ,   the Serplulimab-MSI-H Investigators
Published: October 2022

Abstract
Background
Microsatellite instability-high/mismatch repair-deficient (MSI-H/dMMR) tumours have a high response rate to immunotherapy. Antitumour activity and safety of serplulimab, a novel humanised anti-PD-1 monoclonal antibody, were evaluated in this phase II study.

Methods
In this ongoing, single-arm, open-label, phase II trial, patients with previously treated unresectable or metastatic MSI-H/dMMR solid tumours received intravenous serplulimab 3 mg/kg every 2 weeks for up to 52 cycles. The primary endpoint was objective response rate (ORR) assessed by an independent radiological review committee per Response Evaluation Criteria in Solid Tumors v1.1. Secondary endpoints included additional efficacy measures, safety, and tolerability.

Results
As of 9 January 2021, 108 patients were enrolled, and 68 patients with confirmed MSI-H solid tumours were included in the main efficacy analysis population (MEAP). The median follow-up duration in the MEAP was 7.7 months, with an ORR of 38.2% (95% confidence interval, 26.7–50.8). Of the 108 patients, grade ≥3 treatment-emergent adverse events were reported in 53 (49.1%) patients; immune-related adverse events occurred in 52 (48.1%) patients.

Conclusions
Serplulimab demonstrates a durable antitumour effect and a manageable safety profile in previously treated patients with MSI-H solid tumours. Serplulimab is a promising tissue-agnostic treatment for previously treated MSI-H solid tumours.

Trial registration
NCT03941574.
Filamentous fungal biofilms: Conserved and unique aspects of extracellular matrix composition, mechanisms of drug resistance and regulatory networks in Aspergillus fumigatus
Authors: Liu Shuai , Le Mauff Francois , Sheppard Donald C. , Zhang Shizhu 
Published: October 2022

AbstractThe filamentous fungus Aspergillus fumigatus is an ubiquitous mold that can cause invasive pulmonary infections in immunocompromised patients. Within the lung, A. fumigatus forms biofilms that can enhance resistance to antifungals and immune defenses, highlighting the importance of defining the mechanisms underlying biofilm development and associated emergent properties. A. fumigatus biofilms display a morphology and architecture that is distinct from bacterial and yeast biofilms. Moreover, A. fumigatus biofilms display unique characteristics in the composition of their extracellular matrix (ECM) and the regulatory networks governing biofilm formation. This review will discuss our current understanding of the form and function of A. fumigatus biofilms, including the unique components of ECM matrix, potential drug resistance mechanisms, the regulatory networks governing A. fumigatus biofilm formation, and potential therapeutics targeting these structures.
One-year supplementation with Lactobacillus reuteri ATCC PTA 6475 counteracts a degradation of gut microbiota in older women with low bone mineral density
Authors: Li Peishun , Ji Boyang , Luo Hao , Sundh Daniel , Lorentzon Mattias , Nielsen Jens 
Published: October 2022

AbstractRecent studies have shown that probiotic supplementation has beneficial effects on bone metabolism. In a randomized controlled trial (RCT) we demonstrated that supplementation of Lactobacillus reuteri ATCC PTA 6475 reduced bone loss in older women with low bone mineral density. To investigate the mechanisms underlying the effect of L. reuteri ATCC PTA 6475 on bone metabolism, 20 women with the highest changes (good responders) and the lowest changes (poor responders) in tibia total volumetric BMD after one-year supplementation were selected from our previous RCT. In the current study we characterized the gut microbiome composition and function as well as serum metabolome in good responders and poor responders to the probiotic treatment as a secondary analysis. Although there were no significant differences in the microbial composition at high taxonomic levels, gene richness of the gut microbiota was significantly higher (P < 0.01 by the Wilcoxon rank-sum test) and inflammatory state was improved (P < 0.05 by the Wilcoxon signed-rank test) in the good responders at the end of the 12-month daily supplementation. Moreover, detrimental changes including the enrichment of E. coli (adjusted P < 0.05 by DESeq2) and its biofilm formation (P < 0.05 by GSA) observed in the poor responders were alleviated in the good responders by the treatment. Our results indicate that L. reuteri ATCC PTA 6475 supplementation has the potential to prevent a deterioration of the gut microbiota and inflammatory status in elderly women with low bone mineral density, which might have beneficial effects on bone metabolism.
A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model
Authors: Yu Yangyi , Fischenich Kristine M. , Schoonraad Sarah A. , Weatherford Shane , Uzcategui Asais Camila , Eckstein Kevin , Muralidharan Archish , Crespo-Cuevas Victor , Rodriguez-Fontan Francisco , Killgore Jason P. , Li Guangheng , McLeod Robert R. , Miller Nancy Hadley , Ferguson Virginia L. , Bryant Stephanie J. , Payne Karin A. 
Published: October 2022

AbstractGrowth plate injuries affecting the pediatric population may cause unwanted bony repair tissue that leads to abnormal bone elongation. Clinical treatment involves bony bar resection and implantation of an interpositional material, but success is limited and the bony bar often reforms. No treatment attempts to regenerate the growth plate cartilage. Herein we develop a 3D printed growth plate mimetic composite as a potential regenerative medicine approach with the goal of preventing limb length discrepancies and inducing cartilage regeneration. A poly(ethylene glycol)-based resin was used with digital light processing to 3D print a mechanical support structure infilled with a soft cartilage-mimetic hydrogel containing chondrogenic cues. Our biomimetic composite has similar mechanical properties to native rabbit growth plate and induced chondrogenic differentiation of rabbit mesenchymal stromal cells in vitro. We evaluated its efficacy as a regenerative interpositional material applied after bony bar resection in a rabbit model of growth plate injury. Radiographic imaging was used to monitor limb length and tibial plateau angle, microcomputed tomography assessed bone morphology, and histology characterized the repair tissue that formed. Our 3D printed growth plate mimetic composite resulted in improved tibial lengthening compared to an untreated control, cartilage-mimetic hydrogel only condition, and a fat graft. However, in vivo the 3D printed growth plate mimetic composite did not show cartilage regeneration within the construct histologically. Nevertheless, this study demonstrates the feasibility of a 3D printed biomimetic composite to improve limb lengthening, a key functional outcome, supporting its further investigation as a treatment for growth plate injuries.
Enforced mesenchymal stem cell tissue colonization counteracts immunopathology
Authors: García-Bernal David , Blanquer Miguel , Martínez Carlos M. , García-Guillén Ana I. , García-Hernández Ana M. , Carmen Algueró M. , Yáñez Rosa , Lamana María L. , Moraleda Jose M. , Sackstein Robert 
Published: October 2022

AbstractMesenchymal stem/stromal cells (MSCs) are distributed within all tissues of the body. Though best known for generating connective tissue and bone, these cells also display immunoregulatory properties. A greater understanding of MSC cell biology is urgently needed because culture-expanded MSCs are increasingly being used in treatment of inflammatory conditions, especially life-threatening immune diseases. While studies in vitro provide abundant evidence of their immunomodulatory capacity, it is unknown whether tissue colonization of MSCs is critical to their ability to dampen/counteract evolving immunopathology in vivo. To address this question, we employed a murine model of fulminant immune-mediated inflammation, acute graft-versus-host disease (aGvHD), provoked by donor splenocyte-enriched full MHC-mismatched hematopoietic stem cell transplant. aGvHD induced the expression of E-selectin within lesional endothelial beds, and tissue-specific recruitment of systemically administered host-derived MSCs was achieved by enforced expression of HCELL, a CD44 glycoform that is a potent E-selectin ligand. Compared to mice receiving HCELL−MSCs, recipients of HCELL+MSCs had increased MSC intercalation within aGvHD-affected site(s), decreased leukocyte infiltrates, lower systemic inflammatory cytokine levels, superior tissue preservation, and markedly improved survival. Mechanistic studies reveal that ligation of HCELL/CD44 on the MSC surface markedly potentiates MSC immunomodulatory activity by inducing MSC secretion of a variety of potent immunoregulatory molecules, including IL-10. These findings indicate that MSCs counteract immunopathology in situ, and highlight a role for CD44 engagement in unleashing MSC immunobiologic properties that maintain/establish tissue immunohomeostasis.
Genetic insights into the social organization of Neanderthals
Authors: Skov Laurits , Peyrégne Stéphane , Popli Divyaratan , Iasi Leonardo N. M. , Devièse Thibaut , Slon Viviane , Zavala Elena I. , Hajdinjak Mateja , Sümer Arev P. , Grote Steffi , Bossoms Mesa Alba , López Herráez David , Nickel Birgit , Nagel Sarah , Richter Julia , Essel Elena , Gansauge Marie , Schmidt Anna , Korlević Petra , Comeskey Daniel , Derevianko Anatoly P. , Kharevich Aliona , Markin Sergey V. , Talamo Sahra , Douka Katerina , Krajcarz Maciej T. , Roberts Richard G. , Higham Thomas , Viola Bence , Krivoshapkin Andrey I. , Kolobova Kseniya A. , Kelso Janet , Meyer Matthias , Pääbo Svante , Peter Benjamin M. 
Published: October 2022

AbstractGenomic analyses of Neanderthals have previously provided insights into their population history and relationship to modern humans1–8, but the social organization of Neanderthal communities remains poorly understood. Here we present genetic data for 13 Neanderthals from two Middle Palaeolithic sites in the Altai Mountains of southern Siberia: 11 from Chagyrskaya Cave9,10 and 2 from Okladnikov Cave11—making this one of the largest genetic studies of a Neanderthal population to date. We used hybridization capture to obtain genome-wide nuclear data, as well as mitochondrial and Y-chromosome sequences. Some Chagyrskaya individuals were closely related, including a father–daughter pair and a pair of second-degree relatives, indicating that at least some of the individuals lived at the same time. Up to one-third of these individuals’ genomes had long segments of homozygosity, suggesting that the Chagyrskaya Neanderthals were part of a small community. In addition, the Y-chromosome diversity is an order of magnitude lower than the mitochondrial diversity, a pattern that we found is best explained by female migration between communities. Thus, the genetic data presented here provide a detailed documentation of the social organization of an isolated Neanderthal community at the easternmost extent of their known range.
A minimum data standard for vector competence experiments
Authors: Wu Velen Yifei , Chen Binqi , Christofferson Rebecca , Ebel Gregory , Fagre Anna C. , Gallichotte Emily N. , Sweeny Amy R. , Carlson Colin J. , Ryan Sadie J. 
Published: October 2022

AbstractThe growing threat of vector-borne diseases, highlighted by recent epidemics, has prompted increased focus on the fundamental biology of vector-virus interactions. To this end, experiments are often the most reliable way to measure vector competence (the potential for arthropod vectors to transmit certain pathogens). Data from these experiments are critical to understand outbreak risk, but – despite having been collected and reported for a large range of vector-pathogen combinations – terminology is inconsistent, records are scattered across studies, and the accompanying publications often share data with insufficient detail for reuse or synthesis. Here, we present a minimum data and metadata standard for reporting the results of vector competence experiments. Our reporting checklist strikes a balance between completeness and labor-intensiveness, with the goal of making these important experimental data easier to find and reuse in the future, without much added effort for the scientists generating the data. To illustrate the standard, we provide an example that reproduces results from a study of Aedes aegypti vector competence for Zika virus.