bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Smartphone Usage on Development of Sexuality Among Late Adolescent Boys in Health Sciences Colleges of South India
Authors: Manya Sharath M , K Jayashree , Mithra Prasanna , Galagali Preeti M 
Published: July 2022

Abstract
Purpose
The present study aimed to understand the existing smartphone usage pattern among late adolescents and their association with the development of adolescent male sexuality.
Demography:
Medical, dental, and allied health sciences male students of Asian origin, aged between 18 and 19 years.
Study Settings:
Medical university in Mangalore, an urban area in the South Indian State of Karnataka.
Methodology:
This institution-based cross-sectional study. A pre-validated semi-structured questionnaire (Google form) was electronically mailed to all participants willing to participate in the study, and responses were collected after collecting the informed consent from each participant.
Analysis:
Data analysis was done using IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM Corp. The influence between smartphone usage and sexuality development was done using the Chi-square test and independent-sample t-test. A p-value of < 0.05 was considered statistically significant.
Findings:
Half of the late adolescents have PSU, living with family being a protective factor for PSU. Friends and media remain among the leading sources for raising awareness about male pubertal changes. Pornographic content is accessed through smartphones.
Green tea catechin-grafted silk fibroin hydrogels with reactive oxygen species scavenging activity for wound healing applications
Authors: Lee Gyeongwoo , Ko Young-Gwang , Bae Ki Hyun , Kurisawa Motoichi , Kwon Oh Kyoung , Kwon Oh Hyeong 
Published: July 2022

Abstract
Overproduction of reactive oxygen species (ROS) is known to delay wound healing by causing oxidative tissue damage and inflammation. The green tea catechin, (–)-Epigallocatechin-3-O-gallate (EGCG), has drawn a great deal of interest due to its strong ROS scavenging and anti-inflammatory activities. In this study, we developed EGCG-grafted silk fibroin hydrogels as a potential wound dressing material. The introduction of EGCG to water-soluble silk fibroin (SF-WS) was accomplished by the nucleophilic addition reaction between lysine residues in silk proteins and EGCG quinone at mild basic pH. The resulting SF-EGCG conjugate was co-crosslinked with tyramine-substituted SF (SF-T) via horseradish peroxidase (HRP)/H2O2 mediated enzymatic reaction to form SF-T/SF-EGCG hydrogels with series of composition ratios. Interestingly, SF-T70/SF-EGCG30 hydrogels exhibited rapid in situ gelation (< 30 sec), similar storage modulus to human skin (~ 1000 Pa) and superior wound healing performance over SF-T hydrogels and a commercial DuoDERM® gel dressing in a rat model of full thickness skin defect. This study will provide useful insights into a rational design of ROS scavenging biomaterials for wound healing applications.
Fabrication of an Au-doped Cu/Fe@polymer Nanoreactor with Chemodynamic and Photodynamic Dual Effects as Potential Cancer Therapeutic Agents
Authors: Sun Chun-Kai , Wang Yin-Hsu , Chen Yu-Liang , Lu Ting-Yu , Chen Hsi-Ying , Pan Shih-Chin , Chen Po-Chun , Liao Mei-Yi , Yu Jiashing 
Published: July 2022

Abstract
Nanoparticles are widely used in biomedical applications and cancer treatments due to their minute scale, multi-function, and long retention time. Among the various nanoparticles, the unique optical property derived from the localized surface plasmon resonance effect of metallic nanoparticles is a primary reason that metallic nanoparticles are researched and applied. Copper and Iron nanoparticles have the potential to generate hydroxyl radicals in excess H2O2 via Fenton or Fenton-like reactions. On the other hand, gold nanoparticles equipped with a photosensitizer can transfer the energy of photons to chemical energy and enhance the production of singlet oxygen, which is suitable for cancer treatment. With the actions of these two reactive oxygen species in the tumor microenvironment, cell apoptosis can further be induced.In this work, we first synthesized dual metal nanoparticles with poly[styrene-alt-(maleic acid, sodium salt)( Cu ferrite@PSMA) by a simple one-step hydrothermal reduction reaction. Then, gold(III) was reduced and doped into the structure, which formed a triple metal structure, Au-doped Cu ferrite nanoparticles (Au/Cu ferrite@PSMA NPs). The metal ratio of the product could be controlled by manipulating the Fe/Cu ratio of reactants and the sequence of addition of reactants. The core-shell structure was verified by transmission electron microscopy. Moreover, the hydroxyl radical and singlet oxygen generation ability of Au/Cu ferrite@PSMA was proved. The chemodynamic and photodynamic effect was measured, and the in vitro ROS generation was observed. Furthermore, the behavior of endocytosis by cancer cells could be controlled by the magnetic field. The result indicated that Au/Cu ferrite@PSMA is a potential agent for chemodynamic/photodynamic synergetic therapy.
Prevention of horizontal transfer of laboratory plasmids to environmental bacteria: how to select disinfection methods that are also effective in destroying DNA
Authors: LORET Suzanne Marie , HABIB Boutaina , Romain Pierre , Roba Agnès , Reboul Angéline 
Published: July 2022

Abstract
Routine work of any molecular biology laboratory includes the daily use of microorganisms, including non-pathogenic E coli strains, transformed with a variety of plasmids expressing at least one antibiotic resistance gene (ARG). To check the efficacy of neutralization methods, bacteria isolated from wastewater from laboratories and research institutes were identified using the 16S ribosomal RNA sequencing and they were tested for the presence of an origin of replication and several ARGs frequently found in laboratory plasmids. Surprisingly, the origin of replication of Enterobacteriaceae was detected in non-Enterobacteriaceae bacteria strains suggesting that an interspecies transfer of laboratory plasmids had occurred. Using quantitative Polymerase Chain Reaction (qPCR), we determined the Decimal reduction value (D-value, expressed as concentration of disinfectant or length of physical treatment) of several neutralization methods for their DNA-bd effect: i.e. 0.7M for Sulfuric, 3.2% for a commercial disinfectant P3, 25 minutes for steam sterilization at 121°C and 49 minutes for UVC. A 20-minute treatment of laboratory liquid waste (LLW) with 1–10% sodium chlorine is ineffective in destroying completely plasmid gene markers intreated cells. Therefore, to prevent the horizontal transfer of laboratory ARGs to environmental bacteria, bleach-treated liquid waste should not be released in sewage without additional plasmid destruction treatment.
Exploring The Psycho-emotional Experiences of Women With Infertility In Urban Ghana: A Qualitative Study.
Authors: Otchere Lucinda Naomi , Sefogah Promise Emmanuel , Yarney Lily , Baku Anita Asiwome , Adzei Francis Anderson 
Published: July 2022

Abstract
BackgroundApproximately 20–40% of couples in Sub-Saharan Africa suffer infertility. Infertility is best understood as a socially constructed process where the affected couples define their inability to have children as a problem, its nature and then construct an appropriate course of action. Infertility is often associated with psychological distress among affected couples. The Ghanaian society places high values on childbearing, making infertility a major social problem for the affected women. This subject has limited qualitative data in Ghana.ObjectiveTo explore experiences of women in Ashanti region of Ghana regarding infertility and its effect on daily living.MethodA qualitative study utilizing content analysis approach. Purposive sampling of 18 women with infertility who sought infertility treatment as referral cases in Kumasi, Ashanti Region were selected for a semi-structured in-depth interview after giving informed consent.ResultsFour main themes emerged as participants’ perception of infertility and its consequences on their daily lives. These include: “abuse”, “marital instability”, “social isolation”, and “loss of self-esteem.” The most predominant theme was “loss of self-esteem”. Infertility was found to influences both psychological and social well-being of women.ConclusionInfertility imposes significant impairment on the emotional, psychological and social well-being of women in Ghana. These include: isolation, abuse, loss of self-esteem, and marital instability.What is already known? infertility may impose psychological distress on affected couples.What did study adds: This study reveals the extent to which infertility-related distress affects the daily living experience of women in Sub-Saharan Africa, including physical abuse.How this study might affect research, practice, or policy: The severity of the psycho-emotional burden of infertility is worse on women and could be mitigated if treatment costs are subsidized.
Disruption of C/EBPβ-Clec7a Axis Exacerbates Neuroinflammatory Injury via NLRP3 Inflammasome-mediated Pyroptosis in Experimental Neuropathic Pain
Authors: WU Dan , ZHANG Yanqiong , ZHAO Chunhui , LI Qiuyue , ZHANG Junhong , HAN Jiaxin , XU Zhijian , LI Junfang , MA Yan , WANG Ping , Xu Haiyu 
Published: July 2022

Abstract
Background
Growing evidence shows that C-Type Lectin Domain Containing 7A (Clec7a) may be involved into neuroinflammatory injury of various neurological diseases. However, its roles in neuropathic pain remain unclear.
Methods
A chronic constriction injury (CCI) rat model was established, and gene expression profilings in spinal cord tissues of CCI-insulted rats were detected by both microarray and RNA-seq studies. A series of bioinformatics analyses identified C/EBPβ-Clec7a to be a candidate axis involved into neuropathic pain. Then, its roles in mechanical allodynia, and pathological and molecular changes during CCI progression were determined by various gain-of-function and loss-of-function experiments in vivo and in vitro.
Results
Significant upregulation of Clec7a at both mRNA and protein levels were verified in spinal cord tissues of CCI-insulted rats. Clec7a knockdown markedly attenuated CCI-induced mechanical allodynia, obstructed Syk, ERK and JNK phosphorylation, inhibited NLRP3 inflammasome and caspase-1 activation, GSDMD cleavage, and consequently reduced the release of pro-inflammatory cytokines (all P < 0.05). Mechanically, the rat Clec7a promoter was predicted to bind with transcription factor C/EBPβ, confirmed by Luciferase assay and ChIP-qPCR. Both in vivo and in vitro assays demonstrated that C/EBPβ knockdown significantly suppressed CCI- or LPS/ATP-induced Clec7a upregulation, and subsequently reduced Syk, ERK and JNK phosphorylation, NLRP3 oligomerization, caspase-1 activation, GSDMD expression and pyroptosis, which were markedly reversed by the co-transfection of Clec7a expression vector.
Conclusions
This pre-clinical investigation reveals that C/EBPβ-Clec7a axis may be a potential target for relieving neuropathic pain through alleviating neuroinflammation, paving its way for clinical translation as a promising approach for neuropathic pain therapy.
Antiferromagnetic skyrmion crystal in Janus monolayer CrSi2N2As2
Authors: Dai Ying , Dou Kaiying , Du Wenhui , He Zhonglin , Huang Baibiao , Ma Yandong 
Published: July 2022

Abstract
Antiferromagnetic skyrmion crystal (AF-SkX), a regular array of antiferromagnetic skyrmions, is a fundamental phenomenon in the field of condensed-matter physics. So far, only very few proposals have been made to realize the AF-SkX, and most based on three-dimensional (3D) materials. Herein, using first-principles calculations and Monte-Carlo simulations, we report the identification of AF-SkX in two-dimensional lattice of Janus monolayer CrSi2N2As2. Arising from the broken inversion symmetry and strong spin-orbit coupling, large Dzyaloshinskii–Moriya interaction is obtained in Janus monolayer CrSi2N2As2. This, combined with the geometric frustration of its triangular lattice, gives rise to the skyrmion physics and long-sought AF-SkX in the presence of external magnetic field. More intriguingly, this system presents two different antiferromagnetic skyrmion phases, and such phenomenon is distinct from those reported in 3D systems. Furthermore, by contacting with Sc2CO2, the creation and annihilation of AF-SkX in Janus monolayer CrSi2N2As2 can be achieved through ferroelectricity. These findings greatly enrich the research on antiferromagnetic skyrmions.
Satellite weather data accurately models global space heating and cooling demand
Authors: Staffell Iain , Pfenninger Stefan , Johnson Nathan 
Published: July 2022

Abstract
Various tools exist for modelling the temporal and spatial impacts of weather on energy supply, yet few consider these impacts on energy demand for heating and cooling in the same universal way. Available models are limited in their applicability or validate poorly against measured energy consumption. Here, we present a model of hourly heating and cooling demand that can be applied globally at all scales and customised to specific contexts. We validate it against energy consumption from ~5,000 individual buildings and 43 nations / regions spanning four continents. We then assess two contemporary case studies. Firstly, we show that ‘turning down the thermostat’ by 1°C across all buildings would reduce Europe’s reliance on Russian natural gas imports by one-fifth, and allow the US to increase gas exports by one-tenth. Secondly, we show that providing a ‘comfortable’ standard of space cooling globally would require nearly half of current global electricity demand. The model and underlying data are available freely on an interactive platform to aid further research in this area.
Research on tooth surface design and side milling machining method of ruled line face gear
Authors: chu xiaomeng , zeng hong , wang yanzhong , huang yizhan 
Published: July 2022

Abstract
In order to solve the problem of generalized design and batch processing of face gear, a method of tooth surface design and side milling of ruled-line face gears is proposed in this paper. First of all, the discrete points of the pitch cone surface of the face gear are calculated, and the straight-line cluster and section curve of the tooth surface are constructed to analyze the deviation of the ruled line face gear. Secondly, the tooth surface model of the ruled line face gear is constructed, and the curvature and contact trace are calculated. Thirdly, the curvature and tangent of the ruled line face gear tooth surface are analyzed, the feed plane of the equidistant surface and the guideline are obtained, the torsion angle of the tooth surface and the distribution of the tangent vector along the generatrix are calculated, and the tool axis position based on the two-point offset method is studied. Finally, the side milling processing test of the face gear is carried out, and the tooth surface of the face gear after side milling is measured. The measurement results verify the correctness of the tooth surface design and the side milling processing method of the ruled line face gear.
Development of a species-specific qPCR assay for the detection of invasive African Sharptooth catfish (Clarias gariepinus) using environmental DNA
Authors: Krishnan Gopi , Sunkara Meher , Raghavan Rajeev , Umapathy Govindhaswamy 
Published: July 2022

Abstract
Detection and monitoring of target species is the primary strategy in the management and control of biological invasions. Traditional methods to detect invasive species are time consuming and cumbersome with a requisite for trained taxonomists for identification of aquatic species. Environmental DNA (eDNA)-based molecular methods offer an alternative, as they are quick, cost-effective and requires minimal manpower. In this study, we design and optimize a reliable eDNA-based quantitative PCR assay to detect the African Sharptooth catfish, a highly invasive and banned species in India. Here, we delineate the step-by-step processes involved in the design and optimization of the assay, and show its performance through field-testing in selected water bodies in and around the city of Hyderabad. The present workflow can be used to design assays to detect a wide range of aquatic species.
A case control investigation of COVID-19 associated Mucormycosis in India
Authors: Anand Tanu , Mukherjee Aparna , Satija Aanchal , Velamuri Poonam Sharma , Singh Kh. Jitenkumar , Das Madhuchhanda , Josten Kripa , Yadav Pragya D. , Sahay Rima R. , Keche Archana Y , Nagarkar Nitin M , Gupta Prashant , Reddy D. Himanshu , Mistry Sejal N. , Patel Jimy D. , Rao Prajwal , Rohatgi Shalesh , Ghosh Soumitra , Hazra Avijit , Kindo Anupma Jyoti , Annamalai Radha , Rudramurthy Shivaprakash M , Singh Mini P , Shameem Mohammad , Fatima Nazish , Khambholja Janakkumar R. , Parikh Sangita , Madkaikar Manisha , Pradhan Vandana D. , Kataria Sushila , Sharma Pooja , Panda Samiran , group ICMR- Mucormycosis group ICMR-Mucormycosis 
Published: July 2022

Abstract
Background: Increased occurrence of mucormycosis in India during the second wave of the COVID-19 pandemic in early 2021 in India subsequently prompted us to undertake a multi-site case-control investigation. The objectives were to examine the monthly trend of Covid-19 Associated Mucormycosis (CAM) cases among in-patients and to identify factors associated with it.Methods: Eleven study sites were involved across India and archived records since 1st January till 30th September, 2021 were used for trend analysis. The cases and controls were enrolled during 15th June 2021 to 30th September 2021. Data were collected using a semi-structured questionnaire. Among 1211 enrolled participants, 336 were CAM cases and 875 were COVID-19 positive non-mucormycosis controls. Results: Admitted CAM-case number reached highest point in May 2021 after a month of peak admission for COVID-19. Odds of developing CAM increased with the history of working in a dusty environment (adjusted odds ratio; aOR 3.24, 95%CI: 1.34, 7.82), diabetes mellitus (aOR: 31.83, 95%CI: 13.96, 72.63), longer duration of hospital stay (aOR: 1.06, 95%CI: 1.02, 1.11) and use of methyl prednisolone (aOR: 2.71, 95%CI: 1.37, 5.37) following adjustment for age, gender, occupation, education, type of houses used for living, requirement of ventilatory support and route of steroid administration. Higher proportion of CAM cases required supplemental oxygen compared to the controls; use of non-rebreather mask (NRBM) was associated as a protective factor against mucormycosis compared to face masks (aOR: 0.18, 95%CI: 0.08, 0.41). Genomic sequencing of archived respiratory samples showed similar presence of Delta and Delta derivates in both cases and controls.Conclusions: Appropriate management of hyperglycemia, judicious use of steroids and use of NRBM during oxygen supplementation among COVID-19 patients bear the potential to reduce the risk of occurrence of mucormycosis. Avoiding exposure to dusty environment would add to prevention efforts.
How much is accessibility worth? Utility-based accessibility to evaluate transport policies in Latin America
Authors: Guzman Luis A. , Cantillo-Garcia Victor A. , Oviedo Daniel , Arellana Julian 
Published: July 2022

Abstract
There is now a long tradition of accessibility research in Latin American cities, with an increasingly rich set of frameworks and methods from a distributional perspective. Despite such a positive outlook, most accessibility metrics deployed in research and practice do not consider the dimension of (dis)utility, which resonates more clearly with mainstream transport decision-making and planning. This paper seeks to contribute to debates about the use of utility-based measures as inputs for accessibility assessment of transport infrastructure investments in the Global South using discrete choice modeling and its potential as a bridging language between socially nuanced and economics-driven transport planning practices. This paper uses mixed revealed preferences and stated preferences data collected in Bogotá, Colombia. Then, it uses a logsum accessibility metric to estimate the differentiated impact of a set of infrastructure interventions on the accessibility of residents with different income levels and other socioeconomic conditions for accessing opportunities in the city. Particularly, the logsum accessibility metric analyses future innovations and structural additions to Bogotá public transport networks reflecting their effect on the accessibility and consumer surplus in the next 20 years. The proposed approach captures the benefits derived from the opportunity locations and transport infrastructure improvements, which has relevance for debates about transport policy and practice in this and similar urban contexts in the Global South. These accessibility gains could be assigned a monetary value to include in project cost-benefit assessments.
Low-frequency Signal Generation in Space Based on the High-frequency Electric Antenna Array and Doppler effect
Authors: Cui Anjing , Li Daojing , Wu Jiang , Gao Jinghan , Zhou Kai , Hu Chufeng , Wu Shumei 
Published: July 2022

Abstract
Low-frequency signals can be used in target detection and geological exploration, but the large antenna sizes limit their applications. Hence, it is significant to investigate the low-frequency signal generation method based on antennas with appropriate sizes. Different from mechanical antenna methods, previous work of this paper proposes a novel low-frequency signal generation method in space on the basis of Doppler effect, and designs the high-frequency electric antenna staggered array and timing sequency of the periodic pulse train signal. In this paper, 8-element short-array and 64-element long-array experimental prototypes are proposed, and the 121.35 MHz signal is composed of 156 MHz radiating element signals. Experimental results show that the measured composite signal spectrums are similar to the simulations and physically verify the feasibility of the low-frequency signal generation method based on the high-frequency electric antenna array, which is valuable in the area of low-frequency signal generation with small antennas.
Make it double: Identification and characterization of a Tandem-Hirudin from the Asian medicinal leech Hirudinaria manillensis
Authors: Lukas Phil , Melikian Georgij , Hildebrandt Jan-Peter , Müller Christian 
Published: July 2022

Abstract
Haematophagous leeches expresses a broad variety of secretory proteins in their salivary glands, among them hirudins and hirudin-like factors. Here we describe the identification, molecular and initial functional characterization of Tandem-Hirudin (TH), a novel salivary gland derived factor identified in the Asian medicinal leech, Hirudinaria manillensis. In contrast to the typical structure of hirudins, TH comprises two globular domains arranged in a tandem-like orientation and lacks the elongated C-terminal tail. Similar structures of thrombin inhibitors have so far been identified only in kissing bugs and ticks. Expression of TH was performed in both cell-based and cell-free bacterial systems. A subsequent functional characterization revealed no evidence for a thrombin-inhibitory potency of TH.