bluuebunny/crossref_metadata_2025_split huggingface dataset:
Museum specimens shedding light on the evolutionary history and hidden diversity of the hedgehog family Erinaceidae
Authors: Zeng Ying , He Kai , Chen Xing , Bai Weipeng , Lin Hongzhou , Chen Jianhai , Nedyalkov Nedko , Yamaguchi Nobuyuki , Vijayan Keerthy , Suganthasakthivel Ramamoorthy , Kumar Brawin , Han Yuqing , Chen Zhongzheng , Wang Wenzhi , Liu Yang 
Published: October 2022

Abstract
The family of Erinaceidae comprises 26 extant species in the subfamily Erinaceinae of spiny hedgehogs and the subfamily Galericinae of silky-furred gymnures and moonrats. These animals inhabit various habitats from tropical forests to deserts in Eurasia and Africa. Previous studies hinted that species diversity was likely underestimated. Moreover, erinaceids are among the oldest known living placental mammals originating more than 60 million years ago. The rich fossil records represent both living subfamilies and an extinct subfamily Brachyericinae. Comprehensive understanding of evolutionary history and taxonomic diversity is hampered by the unavailability of samples and the incorporation of molecular and morphological data. Here, we sequenced mitochondrial genomes from museum specimens and morphological data to reconstruct the genealogical relationships of Erinaceidae. Our results finely resolved interspecific relationships of living species and unveiled underestimated species diversity not only in Hylomys as revealed in previous studies, also in Neotetracus gymnures and Atelerix, Hemiechinus, and Paraechinus hedgehogs. The two extinct subfamilies, Brachyericinae and Erinaceinae were supported as sister taxa. There is a hint of a close relationship between fossil Galerix and Southeast Asian Hylomys. These findings highlight the potentiality of museomics but also found an overestimation of divergence times using mitogenomes as revealed in previous studies.
mtRBP complex AMG-1/SLRP-1 mediates germline development and spermatogenesis by maintaining mitochondrial homeostasis in Caenorhabditis elegans
Authors: Wang Peng , Wang Qiushi , Chen Lianwan , Cao Zheng , Zhao Hailian , Su Ruibao , Wang Ning , Ma Xiaojing , Shan Jin , Chen Xinyan , Zhang Qi , Du Baochen , Yuan Zhiheng , Zhao Yanmei , Zhang Xiaorong , Guo Xuejiang , Xue Yuanchao , Miao Long 
Published: October 2022

Abstract
The RNA-binding proteins (RBPs)-mediated post-transcriptional regulation of pre-existing mRNAs is essential for spermatogenesis. But the underlying mechanisms remain poorly understood. Here, we identify a germline-specific mitochondrial RBP AMG-1, a homolog of mammalian LRPPRC, required for spermatogenesis in C. elegans. amg-1 mutation leads to the germline-specific mitochondrial dysfunctions associated with aberrant mitochondrial morphology and structure, which eventually hinders germline development without affecting somatic development. We demonstrate that AMG-1 most frequently binds to mitochondrial DNA-encoded 16S and 12S ribosomal RNAs, the essential components of mitochondrial ribosome, and that 12S rRNA expression mediated by AMG-1 is crucial for germline mitochondrial protein homeostasis. Besides, mitochondrial dysfunction caused by AMG-1 mutation triggers sperm apoptosis in C. elegans. Furthermore, SLRP-1, the homolog of mammalian SLIRP in C. elegans, interacts with AMG-1 genetically to regulate germline development and reproductive success. Taken together, these findings reveal the novel function of a mitochondrial RBP in specifically regulating germline development.
Optimal breastfeeding practice and associated factors among infants aged less than six months in Boke district, eastern Ethiopia: community-based cross-sectional study
Authors: Adem Hassen Abdi , Ahmed Jemal Husein , Usso Ahmedin Aliyi , Baraki Nega , Mesfin Firehiwot 
Published: October 2022

Abstract
Background: Optimal breastfeeding is one of the best and cost-effective strategic interventions to prevent and reduce child death. Globally, it prevents 13% of childhood death, yearly. Few previous studies addressed the levels of domains of optimal breastfeeding practice separately, more emphasized the practice of urban-dwellers and employee mothers’ infants and focused on the practice of infants aged 6-24 months. This study assessed optimal breastfeeding practice and associated factors among infants aged 0-6 months in Boke district in rural eastern Ethiopia.
Method: Community-based cross-sectional study was conducted among 390 randomly selected mother-infant pairs from February 01-30, 2018. Pretested-structured questionnaire was used to collect data from mother-infant pairs. Data were entered using EpiData version 3.1 and analyzed by SPSS version 24. Multivariable logistic regression analysis was used to identify factors associated with optimal breastfeeding practice. P-value<0.05 and AOR (95% CI) were used to report significance and association, respectively.
Results: Optimal breastfeeding practice among infants aged 0-6 months was 55.9% (95%CI: 50.7%, 60.8%). Mothers’ age of 15-24 years (AOR=3.58, 95%CI: 1.06, 11.95) and 25-34 years (AOR=3.49, 95%CI: 1.08, 11.28), having formal education (AOR=2.98, 95%CI: 1.92, 4.62), facility delivery (AOR=1.96, 95%CI: 1.24, 3.12) and good knowledge about breastfeeding (AOR=1.82, 95%CI: 1.16, 2.86) significantly associated with optimal breastfeeding practice.
Conclusions: Prevalence of optimal breastfeeding practice among infants of 0-6 months was low. Youth maternal age, institutional delivery and knowledge on breastfeeding were significant predictors of optimal breastfeeding practice. Promoting institutional delivery and enhancing maternal awareness about breastfeeding practice through existing healthcare services, formal education and Media-outlets would be essential to improve to prevent and reduce the burden of suboptimal breastfeeding practice in the communities.
Cell Entry and Unusual Replication of SARS-CoV-2
Authors: McCann Nathan , Castellino Francis J. 
Published: October 2022

Background:
SARS-CoV-2 is the causative virus for the CoVID-19 pandemic that has frequently mutated to continue to infect and resist available vaccines. Emerging new variants of the virus have complicated notions of immunity conferred by vaccines versus immunity that results from infection. While we continue to progress from epidemic to endemic as a result of this collec-tive immunity, the pandemic remains a morbid and mortal problem.

Objective:
The SARS-CoV-2 virus has a very complex manner of replication. The spike protein, one of the four structural proteins of the encapsulated virus, is central to the ability of the virus to penetrate cells to replicate. The objective of this review is to summarize these complex features of viral replication.

Methods:
A review of the recent literature was performed on the biology of SARS-CoV-2 infection from published work from PubMed and works reported to preprint servers, e.g., bioRxiv and medRxiv.

Results and Conclusion:
The complex molecular and cellular biology involved in SARS-CoV-2 replication and the origination of >30 proteins from a single open reading frame (ORF) have been summarized, as well as the structural biology of spike protein, a critical factor in the cellular entry of the virus, which is a necessary feature for it to replicate and cause disease.
PVA Based Nanofiber Containing GO Modified with Cu Nanoparticles
and Loaded Curcumin; High Antibacterial Activity with Acceleration
Wound Healing
Authors: Ajalli Narges , Pourmadadi Mehrab , Yazdian Fatemeh , Abdouss Majid , Rashedi Hamid , Rahdar Abbas 
Published: October 2022

Background:
The skin is one of the most essential organs of the body that plays a vital role.
Protecting the skin from damage is a critical challenge. Therefore, the ideal wound dressing that has
antibacterial, mechanical, biodegradable, and non-toxic properties can protect the skin against injury
and accelerate and heal the wound.

Objective:
In this study, a nano-wound dressing is designed for the first time. This work is aimed to
optimize and act as a dressing to speed up the wound healing process.

Methods:
Graphene Oxide (GO) was produced by the hummer method. In the next step, GO-copper
(Cu) nanohybrid was prepared, then GO-Cu -Curcumin (Cur) nanohybrid was synthesized. Using the
electrospinning method, polyvinyl alcohol (PVA)/GO-Cu -Cur were spun, and finally, related analyses
were performed to investigate the properties and synthesized chemicals.

Results:
The results showed that the nanocomposite was synthesized correctly, and the diameter of the
nanofibers was 328 nm. The use of PVA improved the mechanical properties. In addition, the wound
dressing had biodegradable, antimicrobial, and non-toxic properties. The results of the scratch test and
animal model showed that this nanocomposite accelerated wound healing and after 14 days showed
92.25% wound healing.

Conclusion:
The synthesized nanocomposite has the individual properties and characteristics of an ideal
wound dressing and replaces traditional methods for wound healing.
Effect of Sickle Cell Trait on Human Immunodeficiency Virus Type 1 Infection
Authors: Okpala Iheanyi , Chukwuka Chinwe , Nouraie Seyed , Nekhai Sergei , Onwuka Chima , Hezekiah Isa , Obodo Onochie , Maisamari Deborah , Okereke Kelechi , Oden Ajake , Tanko Yohanna , Ezekekwu Chinedu , Kwaghi Vivian , Onyedum Cajetan , Nnodu Obiageli 
Published: October 2022

Introduction:
Whereas several studies show that homozygous (HbSS) sickle cell disease protects against human immunodeficiency virus infection, it is not clear if human immunodeficiency virus infection is affected by the heterozygous state of the sickle globin gene (HbAS or sickle cell trait).

Objective:
To evaluate the effects of sickle cell trait on the prevalence and severity of human immunodeficiency virus type 1 infection in a large patient population.

Methods:
Hemoglobin genotype was determined by high performance liquid chromatography (HPLC) in 1,226 HIV-1 patients in Nigeria. Their demographic data were documented. Blood CD4+ cell counts and HIV-1 viral load previously determined on the same blood samples to guide clinical care were used as indices of severity of HIV-1 infection. Statistical analysis of the data was done to evaluate the effects of sickle cell trait on the severity and prevalence of HIV-1 infection, relative to the prevalence of 1.4% in the general population of Nigeria.

Results and Discussion:
The distribution of hemoglobin genotypes among the HIV-1 patients was comparable to that in the general population of Nigeria (Chi-squared statistic =1.025; p value = 0.31, not significant). Neither viral load (p = 0.32) nor blood CD4+ cell count (p = 0.30) was significantly different between all HbAS versus all HbAA patients. There was a trend towards lower viral load in females and a significant interaction between gender and HbAS for viral load (P = 0.018), suggesting that sickle cell trait might be associated with the severity of HIV-1 infection in females.

Conclusion:
The findings suggest that sickle cell trait might be associated with severity of HIV-1 infection in female, but not all, patients. Larger, prospective studies are required to further investigate the effect of sickle cell trait on HIV-1 infection.
An Integrated Reporting Tool for Management of Hiv And Non-communicable Diseases for Primary Health Care Facilities in Limpopo Province, South Africa
Authors: Murudi-Manganye N.S. , Makhado L. , Sehularo L.A. 
Published: October 2022

Introduction:Reporting of a health programme such as integrated management of the Human Immunodeficiency Virus (HIV) and Non-communicable Diseases (NCDs) is essential for programme evaluation to improve patient clinical outcomes. It is a criterion that all services that are provided in primary health care facilities and hospitals are monitored, evaluated and reported according to the approved reporting tools and approved health indicators.Aim:This study aimed to develop a reporting tool to report the implementation of integrated management of HIV and NCDs in Limpopo Province, South Africa.Methodology:The South African National Indicators Data Sets (NIDS) were adopted and used to develop the integrated management of HIV and NCDs reporting tool. A Delphi technique was used to validate the developed reporting tool. Eight (8) HIV and NCDs programme experts participated in the review process to establish the validity of the developed tool.Results:Eight integrated HIV and NCDs data elements reporting tool were developed and reviewed by eight (8) programme experts. The tool was found to be relevant and useful and likely to be adopted by Limpopo province for implementation.Conclusion:This was the first integrated HIV and NCDs reporting tool to be developed for Nurse Initiated Management of Antiretroviral Therapy (NIMART) Nurses in Limpopo Province to serve as a basic reporting tool to improve the integrated management of HIV and NCDs including patient outcomes.
Acceptability and Usability of a Reward-Based Mobile App for Opioid Treatment Settings: Mixed Methods Pilot Study
Authors: Proctor Steven L , Rigg Khary K , Tien Allen Y 
Published: October 2022

Background
Contingency management is an evidence-based yet underutilized approach for opioid use disorder (OUD). Reasons for limited adoption in real-world practice include ethical, moral, and philosophical concerns regarding use of monetary incentives, and lack of technological innovation. In light of surging opioid overdose deaths, there is a need for development of technology-enabled solutions leveraging the power of contingency management in a way that is viewed by both patients and providers as acceptable and feasible.

Objective
This mixed methods pilot study sought to determine the perceived acceptability and usability of PROCare Recovery, a reward-based, technology-enabled recovery monitoring smartphone app designed to automate contingency management by immediately delivering micropayments to patients for achieving recovery goals via smart debit card with blocking capabilities.

Methods
Participants included patients receiving buprenorphine for OUD (n=10) and licensed prescribers (n=5). Qualitative interviews were conducted by 2 PhD-level researchers via video conferencing to explore a priori hypotheses. Thematic analysis of interviews was conducted and synthesized into major themes.

Results
Participants were overwhelmingly in favor of microrewards (eg, US $1) to incentivize treatment participation (up to US $150 monthly). Participants reported high acceptability of the planned debit card spending restrictions (blocking cash withdrawals and purchases at bars or liquor stores, casinos or online gambling). Quantitative data revealed a high level of perceived usability of the PROCare Recovery app.

Conclusions
Patients and providers alike appear receptive to microfinancial incentives in standard OUD treatment practices. Further pilot testing of PROCare is underway to determine acceptability, feasibility, and preliminary effectiveness in a rigorous randomized controlled trial.
The Management Perspective in Digital Health Literature: Systematic Review
Authors: Angerer Alfred , Stahl Johanna , Krasniqi Egzona , Banning Stefan 
Published: October 2022

Background
New digital health technologies are considered one solution to challenges in the health sector, which include rising numbers of chronic diseases and increased health spending. As digitalization in health care is still in its infancy, there are many unanswered questions about the impact of digital health on management.

Objective
This paper assesses the current state of knowledge in the field of digital health from a management perspective. It highlights research gaps within this field to determine future research opportunities.

Methods
A systematic review of digital health literature was conducted using 3 databases. The chosen articles (N=38) were classified according to a taxonomy developed for the purpose, and research gaps were identified based on the topic areas discussed.

Results
The literature review revealed a slight prevalence of practical (n=21, 55%) over theoretical (n=17, 45%) approaches. Most of the papers (n=23, 61%) deal with information technology (IT) and are, therefore, focused more on technology and less on management. The research question in most of the papers (n=31, 82%) deals with the creation of concepts, and very few (n=4, 11%) evaluate or even question existing solutions. Most consider the main reason for digitalization to be the optimization of operational processes (n=26, 68%), and 42% (n=16) deal with new business models. The topic area discussed most frequently was found to be eHealth (n=30, 79%). By contrast, the field of tech health with topics such as sensors receives the least attention (n=3, 8%), despite its significant potential for health care processes and strategy.

Conclusions
Three main research propositions were identified. First, research into digital health innovation should not focus solely on the technology aspects but also on its implications for strategic and operational management. Second, the research community should target other domains besides eHealth. Third, we observed a lack of quantitative research on the real impact of digital health on organizations and their management. More quantitative evidence is required regarding the expected outcome and impact of the implementation of digital health solutions into our health care organizations.
Skills Identification for Healthcare Facility Engineering Technicians
Authors: Call Steven , Hurtado Kristen , Sullivan Kenneth 
Published: October 2022

ABSTRACT
The purpose of this research was to identify the technical skills that are necessary for competency as a facility engineering technician (FET) in the healthcare built environment. The Delphi method was used to draw upon the knowledge and experience of healthcare facility engineering managers and technicians to identify and prioritize baseline technical skills, as well as to identify proficiency levels for the skills. A total of 73 skills were identified as necessary for healthcare FET baseline competency including areas of wood, plastic, and composite; flooring; doors; plaster and gypsum board; mechanical support; fire support; plumbing; electrical; and safety and compliance. Of these 73 skills, 35 were deemed key with strong agreement consensus.
Successfully recruiting, training, and maintaining a competent FET workforce is essential but challenging for healthcare organizations, in part because of the lack of formal education programs and an unclear path of entry into the profession. The results of this research are valuable in that they can be used to develop training for the healthcare FET workforce. Trainers can use the findings of this research when developing curriculum and determining which skills to emphasize. Healthcare organizations can use the findings to assess current capabilities across staff, to determine whether there are gaps in needed skills and to begin developing strategic programs in response to the challenges of attracting and retaining competent FETs. This research suggests other types of jobs need to be examined to move toward developing a more complete understanding of the skills and capabilities necessary for the spectrum of the facility engineering workforce (from technical to managerial) to address attrition, recruitment, retention, and succession challenges.
Estrategias para la adaptación de un sistema de captación de precipitaciones en la vereda de las Palmas del municipio de Tunja (Boyacá)
Authors: Torres Torres Manuel , Escarraga Pachón Luis Fernado , Patiño Pacheco Monica Jovanna 
Published: October 2022

Contextualización En la actualidad el reto de la agricultura es alimentar a una población cada día en ascenso, para ello se debe tener una constante oferta del recurso hídrico para los sistemas productivos. Al día de hoy en el departamento de Boyacá, la productividad agrícola se ha visto perjudicada por el efecto del cambio climático. Esto en relación con los cambios de temperaturas altas durante el día y bajas temperaturas en las noches, con el fenómeno del niño, un aumento en la intensidad y frecuencia de las lluvias para el fenómeno de la niña. Lo que ocasiona pérdidas en la calidad y rendimiento de los cultivos. Por ello, una medida de mitigación es colectar el agua lluvia de techos de viviendas rurales, que puedan ser aprovechadas para utilizarla agua de regadío, agua para sistemas de pecuarios y sistemas hidropónicos o agua para la vivienda rural.
Vacío de conocimiento: La falta de medidas como la recolección de aguas lluvias para sus hogares o para sus sistemas productivos, de implementación de estrategias que podría mejorar la disponibilidad del recurso hídrico en épocas de sequía. Además, No hay estudios sobre captación de aguas lluvias en zonas rurales en Boyacá.
Propósito: Obtener información por medios de datos climatológicos durante dos años para determinar las estrategias para la adaptación de un sistema de captación de precipitaciones y determinar la cantidad de agua captada en una cubierta de una vivienda rural.
Metodología: Se recolectaron los datos de pluviosidad durante dos años, para obtener un volumen aproximado en los episodios invernales. Se analizó datos de factores climáticos, se determinó los datos pluviométricos, el área de captación y el volumen recolectado en fincas.
Resultados y conclusiones: Se obtuvieron los siguientes datos totales por año; para el 2020 se captó 442 mm de agua y en el año 2021 se ha obtenido 357 mm por precipitación y se recogió 64.090 litros o 64,0 metros cúbicos para el año 2020 y 51.165 litros o 51,1 metros cúbicos para año.
Pembelajaran Berbantuan Video Model Latihan Renang Gaya Bebas Terhadap Penguasaan Gaya Bebas dan Kecepatan Renang Siswa Sekolah Dasar
Authors: Dharma Sanjaya Pande Made , Rediani Ni Nyoman 
Published: October 2022

Saat ini kondisi jam sekolah atlet berbenturan dengan jam penggunaan latihan, atlet yang kami miliki hampir semua bersekolah di sekolah negeri yang menerapkan jam sekolah full setengah hari dimana dimulai dari pagi hari yang rata-rata jam masuk sekolah pukul 07.00 Wita. Kemudian keluar sekolah pukul 15.30. belum lagi banyak orang tua yang memberikan pelajaran tambahan terhadap anaknya yang menyebabkan peserta didik mempunyai waktu yang singkat dalam berlatih renang menjadi alsan mengapa penelitian yang bertujuan untuk menganalisis penggunaan pembelajaran berbatuan media video model latihan renang gaya bebas terhadap penguasaan gaya bebas dan kecepatan atlet sekolah dasar. Rancangan penelitian ini adalah quasi-eksperimental design berupa posstest control group design. jumlah populasi penelitian ini adalah 48. Proses pengumpulan data yang digunakan adalah metode tes dan dokumentasi. Metode analisis data penelitian ini adalah analisis deskriptif dan analisis statistik inferensial dengan manova. Hasil penelitian menunjukkan bahwa pembelajaran berbantuan video model latihan renang gaya bebas berpengaruh terhadap penguasaan renang gaya bebas dan kecepatan renang peserta didik baik secara simultan maupun parsial, hal ini ditunjukkan dnegan niali sig. < 0.05. Dari hasil deskritif data juga diperoleh hasil penelitian berupa variabel kecepatan peserta didik lebih dipengaruhi oleh pembelajaran berbasis youtube dibandingkan variabel pemahaman konsep. Sehingga bisa direkomendasikan bahwa pembelajaran berbantuan video model latihan renang gaya bebas bisa digunkan sebagai salah satu pembelajaran inovatif untuk olahraga renang.