bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cultivating learning in vitro: A meta‐ethnography of learning experiences of nursing students regarding high‐fidelity simulation
Authors: Fernández‐Basanta Sara , Picallo‐García Laura , Movilla‐Fernández María‐Jesús 
Published: March 2022

AbstractObjectivesTo synthesise the experience of nursing students in their final years regarding high‐fidelity simulation in acute and critical care.BackgroundFor the complex and changing healthcare environment, new tools are required to help health students, educational staff and managers to design and present rewarding educational simulations. Due to the complexity and limited learning opportunities in real settings, high‐fidelity simulation enables students to acquire skills for the provision of acute and critical care in a controlled environment that closely imitates reality; however, the literature on students’ learning experiences with this education methodology is still limited.DesignThis study followed Noblit and Hare's interpretive meta‐ethnography, which was written and reviewed for reporting clarity against the EQUATOR checklist using the eMERGe.Data sourcesA comprehensive systematic search strategy was carried out in five databases: PubMed, Scopus, CINAHL, Web of Science and PsycINFO.Review methodsTen studies met the research objective and inclusion criteria.ResultsThe metaphor ‘Cultivating learning in vitro’ and four themes were developed to describe the learning experiences of nursing students regarding high‐fidelity simulation in acute and critical care. The themes were as follows: Learning roots—Ways to learn during high‐fidelity simulation; Learning stimulants—Elements that favour learning; Learning impairments—Elements that hinder learning; and Learning flourishing—Results after high‐fidelity simulation.ConclusionsSeeing, doing and reflecting constituted the main sources of learning. Students identified the stimulating and debilitating aspects of learning which could help in the design of simulation sessions and promote their incorporation into nursing curricula. Finally, ‘the flowering of the plant’ represents the learning outcomes developed in a controlled and safe environment.Relevance to clinical practiceThe results of this meta‐ethnography provide keys to promote change in teaching planning in relation to acute and critical care.
In situ purification experiment of a mangrove–aquaculture coupling system: A case study of a crab pond in Wanning, Hainan Province
Authors: Zheng Hao , Ni Sui , Tan Xuemin , Wang Chunlin 
Published: March 2022

AbstractAquaculture development and expansion can destroy and reduce tropical mangrove systems, while discharging sewage into the ocean. This study introduced a new mangrove system into an aquaculture pond to establish a mangrove–aquaculture coupling system. This study aims to reduce pond water eutrophication and decrease nutrient discharge into the ocean without affecting aquaculture. Results show that mangrove planting does not affect Scylla serrata growth (mud crabs), but does stabilize pond environments. While mangrove‐planting inhibits nitrogen and reactive P (PO43−) accumulation in ponds, it is a poor inhibitor of total phosphorus accumulation. The average nitrite (NO2−), reactive P (PO43−), and total nitrogen (TN) contents decreased by 80.46, 35.47, and 31.01%, respectively. This mangrove–aquaculture coupling system provides a method to balance economic development needs with the ecological needs of mangrove wetlands. This study offers suggestions for establishing this system and its associated waste aquaculture pond transformation.
Probiotic characterization of Lactiplantibacillus plantarum HOM3204 and its restoration effect on antibiotic-induced dysbiosis in mice
Authors: Zhang S. , Wang T. , Zhang D. , Wang X. , Zhang Z. , Lim C. , Lee S. 
Published: March 2022

Abstract
The purpose of this study was to evaluate the probiotic characteristics of Lactiplantibacillus plantarum HOM3204 isolated from homemade pickled cabbage and to examine its restoration effect on antibiotic-induced dysbiosis in mice. Lact. plantarum HOM3204 tolerated simulated gastric and intestinal juices with a 99·38% survival rate. It also showed strong adhesion ability (3·45%) to Caco-2 cells and excellent antimicrobial activity against foodborne pathogens in vitro. For safety (antibiotic susceptibility) of this strain, it was susceptible to all the tested seven antibiotics. Lact. plantarum HOM3204 had good stability during storage, especially in cold and frozen conditions. Furthermore, Lact. plantarum HOM3204 significantly restored the gut microbiota composition by increasing the abundance of Lactobacilli and Bifidobacteria and decreasing Enterococci, and improved antioxidative function by raising the concentrations of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in serum of antibiotic-induced dysbiosis in mice. These results suggest that Lact. plantarum HOM3204 could be a potential probiotic as a functional food ingredient.
Antibacterial activity and mechanism of chloroform fraction from aqueous extract of mugwort leaves (Artemisia argyi L.) against Staphylococcus aureus
Authors: Zhang J.-J. , Qu L.-B. , Bi Y.-F. , Pan C.-X. , Yang R. , Zeng H.-J. 
Published: March 2022

Abstract
In this work, the antibacterial activity and mechanism of chloroform fraction obtained from aqueous extract of mugwort leaves against Staphylococcus aureus were investigated. The extract showed obvious antibacterial activity against S. aureus which the minimum inhibitory concentration and minimum bactericidal concentration were determined to be 3·0 and 6·0 mg ml−1 respectively. The mechanism study suggested that the extract could destroy the integrity of the S. aureus cell walls and increase the permeability of cell membrane in a certain concentration, but it could not kill S. aureus in a short time. Instead, the extract could make bacteria in a state of apoptosis for a long time, interfere with the normal physiological metabolism of bacteria, and eventually make bacteria die, which was confirm by scanning electronic microscope.
LncCDCA3L inhibits cell proliferation via a novel RNA structure‐based crosstalk with CDCA3 in hepatocellular carcinoma
Authors: Wang Yongfeng , Liu Yongzhen , Zhang Ting , Guan Guiwen , Mao Tianhao , Liu Hui , Zhang Jing , Lu Fengmin , Chen Xiangmei 
Published: March 2022

AbstractBackground & AimsThe molecular mechanisms underlying hepatocellular carcinoma (HCC) remain poorly understood. In this study, we investigated cell division cycle‐associated 3 (CDCA3) expression status and characterized a CDCA3‐related long non‐coding RNA (lncRNA) in HCC.MethodsRT‐qPCR and western blot were used to determine CDCA3 expression level in HCC clinical specimens. 5′ and 3′‐RACE, RNAscope, RNA pull‐down, CRISPR/Cas9‐based RNA immunoprecipitation (CRIP) and site‐directed mutation experiments were used to characterize lncCDCA3L and investigate its function target. Chi‐square test and Kaplan–Meier analysis were used to assess lncCDCA3L clinical significance. The effects of lncCDCA3L on HCC development were assessed by overexpression in vitro and in vivo.ResultsIn this study, we found CDCA3 was a potential oncogenic factor in HCC and characterized the lncCDCA3L, which could inhibit CDCA3. LncCDCA3L is significantly downregulated in HCC and its expression level is associated with tumour size and can act as an independent risk factor affecting postoperative survival time in HCC patients. Mechanistically, lncCDCA3L can repress CDCA3 protein level and inhibit hepatocarcinogenesis by directly binding to CDCA3 mRNA at 1423–1455 region via a novel manner based on a hairpin structure motif.ConclusionsOur study collectively unveiled the molecular mechanisms of how lncCDCA3L repressed the tumourigenic properties of HCC cells and exhibited a tumour suppressor character in HCC in a CDCA3‐dependent manner. The findings here support lncCDCA3L can be used as a candidate prognostic biomarker for HCC patients.
Does urinary urgency drive urinary frequency in overactive bladder?
Authors: Versi Eboo , Rovner Eric S. , Dmochowski Roger R. , Tu Le Mai , De Wachter Stefan 
Published: March 2022

AbstractObjectivesTo determine if reduction in urgency in patients with refractory overactive bladder syndrome (rOAB) is correlated with a reduction in voiding frequency and symptom bother.MethodsData from a prospective series of women with rOAB undergoing radiofrequency ablation of the subtrigonal tissue, termed selective bladder denervation (SBD), was analyzed. Main outcome measures included urgency voids, nonurgency voids, and urgency urinary incontinence (UUI) epidsodes from a 3‐day voiding diary and quality of life (QoL) measures (Overactive Bladder Questionnaire Short Form [OAB‐q SF]).ResultsThe dataset comprised 62 completer subjects at 6 months. Compared to baseline, UUI and urgency voids were lower (P < .001), but nonurgency voids were increased (P < .001) and QoL measures were improved (P < .001), but functional bladder capacity did not increase. At baseline and 6 months post treatment, symptom bother was correlated with urgency voids (P < .05) but inversely correlated with nonurgency voids (P < .001). Urgency and nonurgency voids were inversely related at baseline and at 6 months (P < .0001). Following treatment, urgency voids decreased by 41% and nonurgency voids increased by 33%.ConclusionsAs with other interventions for OAB, SBD resulted in a reduction in OAB symptoms and an improvement in QoL measures. However, the decrease in urgency voids was offset by an increase in nonurgency voids, suggesting that habit or defensive voiding is a significant driver of urinary frequency. It is not certain if this is a unique finding of the SBD effect, but these data do suggest that analyses of outcomes of other OAB interventions are warranted to gain a better understanding of the genesis of OAB symptoms.
Improving nutrition budgeting in health sector plans: Evidence from India's anaemia control strategy
Authors: Saini Avi , Shukla Ritwik , Joe William , Kapur Avani 
Published: March 2022

AbstractIn India, 15 nutrition interventions are delivered and financed through the National Health Mission (NHM). Programmatic know‐how, however, on tracking nutrition budgets in health sector plans is limited. Following the four phases of the budget cycle—planning, allocations, disbursements and expenditure, this paper presents a new method developed by the authors to track nutrition budgets within health sector plans. Using the example of the Anemia Mukt Bharat (AMB) or Anemia Free India strategy, it reports preliminary findings on the application of the first two phases of the method, that is, to track and act for improved planning and allocations, covering 12 states. The paper lists out the budget heads, cost norms and developed tools to plan adequately. Supportive action was undertaken through sharing trends and trainings for AMB's budgeting to create opportunities for improvements. It was observed that the AMB budget increased over 3 years despite the COVID situation. It increased from INR 6184 million in FY 2019–2020 to INR 6293 million, a 2% increase in FY 2020–2021, and to INR 7433 million, an 18% increase in FY 2021–2022. The difference in allocations and planned budgets were low (16%, 4% and 11%, respectively) while the difference in required budgets and planned budgets were significant but reduced consistently (41%, 31% and 22%, respectively). The paper concludes that the methods adopted for tracking and acting for improved nutrition budgets helped in informing national and state governments regarding yearly trends. Such methods can be effective and be developed for other nutrition interventions.
Faster‐haplodiploid evolution under divergence‐with‐gene‐flow: Simulations and empirical data from pine‐feeding hymenopterans
Authors: Bendall Emily E. , Bagley Robin K. , Sousa Vitor C. , Linnen Catherine R. 
Published: March 2022

AbstractAlthough haplodiploidy is widespread in nature, the evolutionary consequences of this mode of reproduction are not well characterized. Here, we examine how genome‐wide hemizygosity and a lack of recombination in haploid males affects genomic differentiation in populations that diverge via natural selection while experiencing gene flow. First, we simulated diploid and haplodiploid “genomes” (500‐kb loci) evolving under an isolation‐with‐migration model with mutation, drift, selection, migration and recombination; and examined differentiation at neutral sites both tightly and loosely linked to a divergently selected site. As long as there is divergent selection and migration, sex‐limited hemizygosity and recombination cause elevated differentiation (i.e., produce a “faster‐haplodiploid effect”) in haplodiploid populations relative to otherwise equivalent diploid populations, for both recessive and codominant mutations. Second, we used genome‐wide single nucleotide polymorphism data to model divergence history and describe patterns of genomic differentiation between sympatric populations of Neodiprion lecontei and N. pinetum, a pair of pine sawfly species (order: Hymenoptera; family: Diprionidae) that are specialized on different pine hosts. These analyses support a history of continuous gene exchange throughout divergence and reveal a pattern of heterogeneous genomic differentiation that is consistent with divergent selection on many unlinked loci. Third, using simulations of haplodiploid and diploid populations evolving according to the estimated divergence history of N. lecontei and N. pinetum, we found that divergent selection would lead to higher differentiation in haplodiploids. Based on these results, we hypothesize that haplodiploids undergo divergence‐with‐gene‐flow and sympatric speciation more readily than diploids.
‘The last thing you feel is the self‐disgust’. The role of self‐directed disgust in men who have attempted suicide: A grounded theory study
Authors: Mason David , James Darren , Andrew Liz , Fox John R. E. 
Published: March 2022

AbstractObjectiveGlobally, suicide affects more men than women. Emotional pain underpins many theoretical accounts of suicidality, yet little is known about the role of disgust in suicide. Self‐directed disgust, whereby aspects of the self‐serve as an object of disgust, has been hypothesised to factor in suicide. This research aimed to explore the processes which link self‐disgust to attempted suicide in males.MethodNine men who had attempted suicide completed semi‐structured interviews. The interview data were analysed using a constructivist grounded theory methodology.ResultsThree concepts emerged out of the analysis: (1) self‐disgust; (2) worthlessness; and (3) the endured emotional distress of ‘the abyss’” – these concepts interweaved, leading the men to experience hopelessness, disconnection and an inability to cope, leading ultimately to their suicide attempt. Throughout this journey, various disgust‐related processes worsened men's distress and increased their suicidal risk. Historic adversities prevailed across the data, as did the men's difficulties in understanding their emotions.ConclusionSelf‐disgust was an important emotion in the men's experiences of suicide and shaped their views of themselves and their lives. The distancing and repellent properties of self‐disgust, in addition to the fear of having their ‘disgustingness’ exposed, increased suicidal risk. Self‐disgust appeared more pervasive in the suicidality of men with a history of multiple childhood adversities. The limitations of this research are discussed as implications for clinical practice and directions for future research.
Set up from the beginning: The origin and early development of cassava storage roots
Authors: Carluccio Anna Vittoria , David Laure C. , Claußen Joelle , Sulley Marco , Adeoti Seun Raheemat , Abdulsalam Toyin , Gerth Stefan , Zeeman Samuel C. , Gisel Andreas , Stavolone Livia 
Published: March 2022

AbstractDespite the importance of storage root (SR) organs for cassava and the other root crops yield, their developmental origin is poorly understood. Here we use multiple approaches to shed light on the initial stages of root development demonstrating that SR and fibrous roots (FR) follow different rhizogenic processes. Transcriptome analysis carried out on roots collected before, during and after root bulking highlighted early and specific activation of a number of functions essential for root swelling and identified root‐specific genes able to effectively discriminate emerging FR and SR. Starch and sugars start to accumulate at a higher rate in SR before they swell but only after parenchyma tissue has been produced. Finally, using non‐destructive computed tomography measurements, we show that SR (but not FR) contain, since their emergence from the stem, an inner channel structure in continuity with the stem secondary xylem, indicating that SR derive from a distinct rhizogenic process compared with FR.
Effects of a multimodal approach to food art therapy on people with mild cognitive impairment and mild dementia
Authors: Lee Hyojin , Kim Eunjoo , Yoon Ju Young 
Published: March 2022

AbstractBackgroundFood art therapy (FAT) has multiple modalities in which cognition, emotion, and social changes are stimulated. The purpose of this study was to design a multimodal approach to a food art therapy (MM‐FAT) program and identify its effects on cognitive ability, daily living functioning, depression, self‐esteem, self‐efficacy, self‐expression, and social functioning in individuals with mild cognitive impairment (MCI) and mild dementia by employing a mixed methods research design.MethodsThe participants included 39 patients from a public dementia care centre in Seoul, Korea. The intervention group, which comprised 20 participants, received 12 MM‐FAT sessions 3 times a week for 4 weeks, and the control group, which included 19 participants, received usual care. The MM‐FAT program was evaluated based on its effectiveness on cognitive, daily living, emotional, and social functioning outcome measures at three time points using repeated measures analysis of variance. Semi‐structured interviews (n = 9) were conducted to evaluate the overall experience of the MM‐FAT program and its outcomes.ResultsThe findings reveal that MM‐FAT has a positive effect on the cognitive, emotional, and social functioning of individuals with MCI and mild dementia. However, there were no enhancements in individuals' daily living functioning, and the lasting effects of the intervention could not be assessed. Cognition and depression increased significantly at the end of the MM‐FAT program. Self‐expression and self‐efficacy were significantly higher in the MM‐FAT group than in the control group. The semi‐structured interviews revealed improvements in participants' behaviour, communication, and interaction.ConclusionThis mixed methods study focused on individuals with MCI or mild dementia contributes to an understanding of the effectiveness of a FAT program employing a multimodal approach. Despite the COVID‐19 pandemic, the study was able to enrich the effects of MM‐FAT on cognitive, emotional, and social functioning through qualitative findings.
Bonding Social Capital of Rural Women in Southwest Iran: Application of Social Network Analysis*
Authors: Ghorbani Mehdi , Javadi Seyed Akbar , Rasekhi Sare , Yazdanparast Maryam , Azadi Hossein 
Published: March 2022

AbstractThe establishment of local communal organizations is a unique strategy of rural communities to build resilience against economic challenges. Shirvareh is traditionally recognized as an important communal organization of women in Southwestern Iran for managing dairy production and securing their livelihood. Preserving this vital social institution and its associated indigenous knowledge was the main motivation of this study to examine factors influencing Shirvareh’s performance. Given that relevant studies on the performance of this social organization in Iran are rare, the current study was an attempt to fill this gap through an empirical study using a social network analysis (SNA) approach. The general goal of this study was to investigate the networks of trusting relationships of rural women involved in the Shirvareh organization in Ghasr‐e‐Yaghoub village located in Fars province, Southwestern Iran. The results revealed that possible in‐group trust ties were maximum whereas out‐group trust ties were moderate to high, meaning that bonding social capital at individual, sub‐group, and network level are generally high. High in‐group and out‐group ties enable community members to cope with disturbances, initiate social innovation, and act collectively, which in this case, assures the sustainability of their efficient traditional organization and protects them against poverty.
Responsible digital agri‐food innovation in Australian and New Zealand public research organisations
Authors: Espig Martin , Fielke Simon , Finlay‐Smits Susanna C. , Jakku Emma , Turner James A. , Robinson Cathy J. , Hunter Cass , Lacey Justine 
Published: March 2022

AbstractThis article analyses digital agricultural technologies (agtech) innovation in two public research organisations in Australia and Aotearoa New Zealand through the lens of responsible innovation (RI), and how corresponding activities were realised in practice. Four virtual workshops explored the operationalisation of RI and its efficacy in digital agtech development. Thematic analysis of workshop materials provided scope to assess the recognised and realised value of RI in both digital agtech programmes, which we found lagging behind RI's full, perhaps idealised, potential. The value proposition of RI can, therefore, not be taken for granted without support for its operationalisation and institutionalisation. Given growing demands on public research organisations to responsibly develop transformational research and innovation, the article outlines recommendations for turning RI aspirations into situated research practices. We conclude that the next phase of agtech innovation should align with the co‐evolution of rural social research and its contribution to more responsible digital agtech innovation.
Do nonhuman animals reason about prestige‐based status?
Authors: Mandalaywala Tara M. 
Published: March 2022

AbstractStatus is a complex, but crucially important, aspect of life across species. In recent decades, researchers have made significant contributions to our understanding of both the pathways by which status can be attained, as well as our underlying capacities for reasoning about these pathways. In 2001, Henrich & Gil‐White proposed a prestige‐based pathway to status where low status actors willingly defer to competent or knowledgeable high status actors, as a means of facilitating social learning and cultural transmission. Although this type of status hierarchy, and the capacity to reason about it, was hypothesized to be unique to humans, here I argue that there are several reasons why we might observe prestige‐based status, and the capacity for reasoning about this pathway to status, in some nonhuman species as well. These reasons focus on the prevalence, importance, and sophistication of social learning in certain taxa, as well as the marked variation in hierarchy characteristics and structure across species. I point out places where our current methodologies encounter difficulties distinguishing whether a hierarchy in the wild is based on dominance or prestige, where our experimental methods leave us unable to assess whether an individual is reasoning about a high status actor as being prestigious or formidable, and provide suggestions for addressing these limitations. Adopting a comparative approach will clarify whether prestige‐based status truly is unique to humans, and—if not—precisely what selective pressures facilitate the emergence of prestige‐based status and the capacity for reasoning about it.