bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison Between Gradients and Parcellations for Functional Connectivity Prediction of Behavior
Authors: Kong Ru , Rui Tan Yan , Wulan Naren , Qi Rong Ooi Leon , Farahibozorg Seyedeh-Rezvan , Harrison Samuel , Bijsterbosch Janine D. , Bernhardt Boris C. , Eickhoff Simon , Thomas Yeo B.T. 
Published: September 2022

AbstractResting-state functional connectivity (RSFC) is widely used to predict behavioral measures. To predict behavioral measures, representing RSFC with parcellations and gradients are the two most popular approaches. Here, we compare parcellation and gradient approaches for RSFC-based prediction of a broad range of behavioral measures in the Human Connectome Project (HCP) and Adolescent Brain Cognitive Development (ABCD) datasets. Among the parcellation approaches, we consider group-average “hard” parcellations (Schaefer et al., 2018), individual-specific “hard” parcellations (Kong et al., 2021a), and an individual-specific “soft” parcellation (spatial independent component analysis with dual regression; Beckmann et al., 2009). For gradient approaches, we consider the well-known principal gradients (Margulies et al., 2016) and the local gradient approach that detects local RSFC changes (Laumann et al., 2015). Across two regression algorithms, individual-specific hard-parcellation performs the best in the HCP dataset, while the principal gradients, spatial independent component analysis and group-average “hard” parcellations exhibit similar performance. On the other hand, principal gradients and all parcellation approaches perform similarly in the ABCD dataset. Across both datasets, local gradients perform the worst. Finally, we find that the principal gradient approach requires at least 40 to 60 gradients to perform as well as parcellation approaches. While most principal gradient studies utilize a single gradient, our results suggest that incorporating higher order gradients can provide significant behaviorally relevant information. Future work will consider the inclusion of additional parcellation and gradient approaches for comparison.
A SHR-IDD-PIN regulatory network mediates minor vein differentiation in rice
Authors: Liu Qiming , Teng Shouzhen , Deng Chen , Wu Suting , Li Haoshu , Wang Yanwei , Wu Jinxia , Cui Xuean , Zhang Zhiguo , Quick William Paul , Brutnell Thomas P. , Sun Xuehui , Lu Tiegang 
Published: September 2022

ABSTRACTC3 and C4 grasses directly and indirectly provide the vast majority of calories to the human diet, yet our understanding of the molecular mechanisms driving photosynthetic productivity in grasses is largely unexplored. Here we define a genetic circuit comprised of SHR, IDD and PIN family members that specify vascular identify and ground cell proliferation in leaves of both C3 and C4 grasses. Ectopic expression and loss-of-function mutant studies of SHORT ROOT (SHR) paralogs in C3Oryza sativa (rice) and C4Setaria viridis (green millet) revealed a role in both minor vein formation and ground cell differentiation. Genetic and in vitro studies further suggest that SHR regulates this process through its interaction with Indeterminate Domain (IDD) IDD 12 and 13. We further show a direct interaction of these IDD proteins with a putative regulatory element within the auxin transporter PIN5c gene. Collectively, these studies indicated that a SHR-IDD regulatory circuit mediates auxin flow through the negative regulation of PIN protein expression to modulate minor vein patterning in the grasses.
Investigation of reversible histone acetylation and dynamics in gene expression regulation using 3D liver spheroid model
Authors: Stransky Stephanie , Cutler Ronald , Aguilan Jennifer , Nieves Edward , Sidoli Simone 
Published: September 2022

AbstractBackgroundThree-dimensional (3D) cell culture has emerged as an alternative approach to 2D flat culture to model more accurately the phenotype of solid tissue in laboratories. Culturing cells in 3D more precisely recapitulates physiological conditions of tissues, as these cells reduce activities related to proliferation, focusing their energy consumption towards metabolism and homeostasis.ResultsHere, we demonstrate that 3D liver spheroids are a suitable system to model chromatin dynamics and response to epigenetics inhibitors. To delay necrotic tissue formation despite proliferation arrest, we utilize rotating bioreactors that apply active media diffusion and low shearing forces. We demonstrate that the proteome and the metabolome of our model resemble typical liver functions. We prove that spheroids respond to sodium butyrate (NaBut) treatment, an inhibitor of histone deacetylases (HDACi), by upregulating histone acetylations and transcriptional activation. As expected, NaBut treatment impaired specific cellular functions, including the energy metabolism. More importantly, we demonstrate that spheroids reestablish their original proteome and transcriptome, including pre-treatment levels of histone acetylation, metabolism, and protein expression once the standard culture condition is restored after treatment. Given the slow replication rate (>40 days) of cells in 3D spheroids, our model enables to monitor the recovery of approximately the same cells that underwent treatment, demonstrating that NaBut does not have long-lasting effects on histone acetylation and gene expression. These results suggest that histone acetylation has minimal epigenetics memory in our spheroids culture.ConclusionTogether, we established an innovative cell culture system that can be used to model anomalously decondensing chromatin in physiological cell growth and rule out epigenetics inheritance if cells recover the original phenotype after treatment. The transient epigenetics effects demonstrated here highlights the relevance of using a 3D culture model system that could be very useful in studies requiring long term drug treatment conditions that would not be possible using a 2D cell monolayer system.
Growth cone advance requires EB1 as revealed by genomic replacement with a light-sensitive variant
Authors: Dema Alessandro , Charafeddine Rabab , Rahgozar Shima , van Haren Jeffrey , Wittmann Torsten 
Published: September 2022

AbstractA challenge in analyzing dynamic intracellular cell biological processes is the dearth of methodologies that are sufficiently fast and specific to perturb intracellular protein activities. We previously developed a light-sensitive variant of the microtubule plus end tracking protein EB1 by inserting a blue light-controlled protein dimerization module between functional domains. Here we describe an advanced method to replace endogenous EB1 with this light-sensitive variant in a single genome editing step enabling this approach in human induced pluripotent stem cells and derived neurons. We demonstrate that acute and local optogenetic EB1 inactivation in developing cortical neurons induces microtubule depolymerization in the growth cone periphery and subsequent neurite retraction. In addition, advancing growth cones are repelled from areas of blue light exposure. These phenotypes were independent of the neuronal EB1 homolog EB3, revealing a direct dynamic role of EB1-mediated microtubule plus end interactions in neuron morphogenesis and neurite guidance.
Identification and structural modeling of the chlamydial RNA polymerase omega subunit
Authors: Cheng Andrew , Wan Danny , Ghatak Arkaprabha , Wang Chengyuan , Feng Deyu , Fondell Joseph D. , Ebright Richard H. , Fan Huizhou 
Published: September 2022

ABSTRACTGene transcription in bacteria is carried out by the multisubunit RNA polymerase (RNAP), which is composed of a catalytic core enzyme and a promoter-recognizing σ factor. RNAP core enzyme comprises two α subunits, one β subunit, one β’
s subunit, and one ω (omega) subunit. Across multiple bacterial taxa, the RNAP ω subunit plays critical roles in the assembly of RNAP core enzyme and in other cellular functions, including regulation of bacterial growth, stress response, and biofilm formation. However, for several intracellular bacterium, including the obligate intracellular bacterium Chlamydia, no RNAP ω subunit previously has been identified. Here, we report the identification of Chlamydia trachomatis hypothetical protein CTL0286 as the chlamydial RNAP ω ortholog, based on sequence, synteny, and AlphaFold and AlphaFold-Multimer three-dimensional-structure predictions. We conclude that CTL0286 functions as the previously missing chlamydial ω ortholog. Extensions of our analysis indicate that all obligate intracellular bacteria have ω orthologs.IMPORTANCEChlamydiae are common mammalian pathogens. Chlamydiae have a unique developmental cycle characterized with an infectious but nondividing elementary body (EB), which can temporarily survive outside host cells, and a noninfectious reticulate body (RB), which replicates only intracellularly. Chlamydial development inside host cells can be arrested during persistence in response to adverse environmental conditions. Transcription plays a central role in the progression of the chlamydial developmental cycle as well as entry into and recovery from persistence. The identification of the elusive ω subunit of chlamydial RNAP makes possible future study of its regulatory roles in gene expression during chlamydial growth, development, and stress responses. This discovery also paves the way to prepare and study the intact chlamydial RNAP and its interactions with inhibitors in vitro.
Role of vitamin D in the academic performance of health sciences students in Saudi Arabia
Authors: AlZahrani Wala Ibrahim , Oommen Anitha 
Published: September 2022

PurposeMany researchers have reported that vitamin D can affect brain development as well as brain function. The prevalence of vitamin D deficiency in the Saudi population is 81% and it is more among women than among men. Though many studies have been done to find out the factors influencing the academic performance of Health sciences students, there is not adequate evidence regarding the influence of vitamin D level on academic performance. Therefore, this study aims to find out the association if any, between the vitamin D level and academic performance of health sciences students.Design/methodology/approachAfter obtaining the ethical committee approval, the data was collected from 86 female medical students, 70 female applied medical sciences students and 57 nursing students of Northern Border University. The detailed questionnaire contained the aim of the study, demographic characteristics and academic performance predictors such as self-efficacy, academic motivation, academic engagement and social engagement. The vitamin D levels were measured by an enzyme-linked immunosorbent assay (ELISA) machine (BioTek) which is available in the local hospital. The multiple linear regression analysis was used to find out the association between vitamin D levels and academic performance.FindingsThis study showed that vitamin D level had a significant association with the overall performance of the students as well as their self-efficacy.Research limitations/implicationsSince there is a lot of stress among health sciences students due to subject overload and inadequacy of time, the health aspects are often overlooked. This study emphasizes the importance of early screening of vitamin D levels and early intervention in those with low vitamin D levels for better academic performance.Social implicationsThere is very little awareness of the impact of vitamin D deficiency on academic motivation, academic engagement, social engagement and self-efficacy among medical and health sciences students. This study can increase awareness.Originality/valueThere are very few studies done to find out the association between Vitamin D level and academic performance. This study is unique as it has highlighted the association between vitamin D level and grade point average (GPA) and also the association between vitamin D level and academic predictors such as self-efficacy, academic motivation, academic engagement and social engagement.
Resource integration capabilities to enable platform complementarity in healthcare service ecosystem co-creation
Authors: Pikkarainen Minna , Kemppainen Laura , Xu Yueqiang , Jansson Miia , Ahokangas Petri , Koivumäki Timo , Hong Gu He , Francis Gomes Julius 
Published: September 2022

PurposeCovid has increased the usage of multisided digital platforms. For companies, this has become a business opportunity. Data usage on platforms requires that platform companies co-create services for common customers. In this case, the target is not to make the same value proposition but rather to use the resources such as data, knowledge, technology, or institutions in a complementary manner. Platforms are characterized as a combination of hardware and software including standards, interfaces, and rules making it possible for different ecosystem players to complement and interact in the ecosystem. Current ecosystems include several platforms that do not work without resource integration. The purpose of this study is to increase understanding what do we mean by resource complementarity in service ecosystems.Design/methodology/approachThis study was done via an in-depth qualitative case study in which a health service ecosystem co-creating technological surgery innovation was used as a unit of analysis.FindingsThe authors’ findings suggest that key resource capabilities, to enable complementarity in service ecosystems, are motivation, knowledge, skills, data and complementary designed technology components.Research limitations/implicationsThe authors’ study increases theoretical understanding of what does one mean by construct of resource complementarity.Practical implicationsFrom a managerial perspective, it is shown that organizations need to develop adaptive capabilities to utilize internal and external competencies and resources and enable co-creative processes within the service ecosystem.Originality/valueVery few empirical studies in the marketing literature have focused on multi-sided digital platforms and their resource complementarity in the data-driven healthcare ecosystem settings.
Integration of artificial intelligence of things (AIoT) in the public sector: drivers, barriers and future research agenda
Authors: Ishengoma Fredrick R. , Shao Deo , Alexopoulos Charalampos , Saxena Stuti , Nikiforova Anastasija 
Published: September 2022

PurposeWith the development of information technology (IT), governments around the globe are using state-of-the-art IT interfaces to implement the so-called 3E’s in public service delivery, that is, economy, efficiency and effectiveness. Two of these IT interfaces relate to Artificial Intelligence (AI) and Internet of Things (IoT). While AI focuses on providing a “human” garb for computing devices, thereby making them “intelligent” devices, IoT relies on interfaces between sensors and the environment to make “intelligent” decisions. Recently, the convergence of AI and IoT – also referred to as Artificial Intelligence of Things (AIoT) – is seen as a real opportunity to refurbish the public service delivery formats. However, there is limited understanding as to how AIoT could contribute to the improvisation of public service delivery. This study aims to create a modular framework for AIoT in addition to highlighting the drivers and barriers for its integration in the public sector.Design/methodology/approachThis descriptive-explanatory study takes a qualitative approach. It entails a thorough examination of the drivers and barriers of integrating AI and IoT in the public sector. A review of literature has led to the development of a conceptual framework outlining the various factors that contribute to creating public value.FindingsValue creation occurs when AI and IoT coalesce in the public service delivery mechanisms.Originality/valueAIoT is a cutting-edge technology revolutionizing health care, agriculture, infrastructure and all other industrial domains. This study adds to the growing body of knowledge on the public sector's use of AI and IoT. Understanding these disruptive technologies is critical to formulating policies and regulations that can maximize the potential benefits for the public-sector organizations.
A new thermal protective clothing design with silica aerogel filled acrylic nanofibers
Authors: Altay Pelin , Eryürük Selin Hanife , Özcan Gülay , Öztürk Didem , Saral Melda Naz , Altınordu Çağla 
Published: September 2022

PurposeFirefighters are exposed to high outdoor temperature and heat stress caused by metabolic activities during firefighting and should wear protective clothing to ensure their safety and health. Traditional firefighter protective suits are bulky and heavy garments with reduced thermal comfort properties since the fabric thickness and moisture barrier layers prevent heat transfer of the garment and cause additional heat stress. The aim of this study is to reduce heat stress by creating a new fabric design with silica aerogel membrane as a moisture barrier for three-layer fabric system.Design/methodology/approachPolyacrylonitrile (PAN) nanofibers were produced with three different silica aerogel contents and used for three-layered clothing system as a moisture barrier for giving desired protectiveness and thermal comfort to firefighters. Different fabric combinations were designed using two types of outer shell fabrics, two types of moisture barrier fabrics, two types of thermal barrier fabrics and PAN/silica aerogel membranes.FindingsThe results show that a lighter fabric system with improved wearer’s mobility and thermal comfort properties (thermal resistance and moisture permeability) is achieved with the use of PAN/silica aerogel membrane as an intermediate layer compared to commercial thermal protective fabric systems.Originality/valueDifferently from traditional thermal protective clothing, which may not provide adequate protection in long-term heat conditions or when exposed to flash fire, a new thermal protective clothing has been developed to be used in extremely hot environments, providing desired technical and performance properties, ease to wear comfort.
CSR disclosure and debt financing in India: does CEO tenure matter?
Authors: Oware Kofi Mintah , Appiah Kingsley , Adomah Worae Thomas 
Published: September 2022

PurposeThe study aims to examine whether corporate social responsibility (CSR) disclosure does improve debt financing of listed firms with sustainable development agendas coupled with high chief executive officer (CEO) tenure in India.Design/methodology/approachEmploying panel regression based on fixed effect and instrumental variable regression with fixed effect assumptions, the study examined data from the Bombay stock exchange from the period 2010 to 2019.FindingsThe study demonstrates that the disclosure of current exchange capital and moral capital cannot cause a firm to access short-term and long-term debt financing. However, lag investment in moral capital causes a positive effect on short-term debt financing. The second findings show that CEO tenure has a positive and statistically significant association with short-term debt financing and an insignificant association with long-term debt financing. The third findings show that the interaction of current CSR disclosure (moral and exchange capital) and CEO tenure is insignificant in affecting short-term and long-term debt finance. However, the interaction of lag CSR disclosure (moral and exchange capital) and CEO tenure positively affect short-term debt financing. The study addresses any endogeneity concerns arising from the CSR disclosure-debt financing association.Research limitations/implicationsThis study uses a single country to examine the inter-relationship between CEO tenure and debt financing and CSR measured by moral capital and exchange capital, thereby limiting the study's results for generalisation.Practical implicationsThe observation is that moral capital investment and disclosure do not guarantee new entrants the chance to access debt financing, but subsequent and lag CSR disclosure ensures access.Originality/valueNo studies examine morality from CSR disclosure on debt financing. This study shows that decoupling CSR into exchange capital and moral capital in accessing debt financing presents new inputs for scholarly debate on CSR.
Theories applied in corporate voluntary disclosure: a literature review
Authors: Rouf Md. Abdur , Siddique Md. Nur-E-Alam 
Published: September 2022

PurposeThis paper attempts to review the corporate voluntary disclosure (CVD) from the theoretical perspective as well as propose a conceptual framework.Design/methodology/approachThe researchers use structural literature review technique. The sample literature consisting of 55 articles was extracted from the Scopus database over the period of 2017–2021.FindingsThe literature observes that the legitimacy, agency and stakeholder theories are most applied in CVD related studies than the other theories. It is also revealed that researchers need to concentrate more studies on those theories of CVD that have been applied in a limited study such as neo-institutional, signaling, resource dependence, political economy and impression management theories.Practical implicationsThe findings can help the understanding of parties such as practitioners', regulators and potential investors of the theories in CVD from a combined and comprehensive view.Social implicationsThe results of the study offer new insights into the potential impact of organizational level and country level theories in CVD from different perspectives of developed and developing countries.Originality/valueThis study delivers an inclusive literature review of the current study approach on the theories of CVD and highlights some stimulating guidelines for future study.
Meta-analysis of augmented reality marketing
Authors: Kumar Harish , Gupta Parul , Chauhan Sumedha 
Published: September 2022

PurposeAmidst the ambiguity about the impact of augmented reality (AR) attributes on hedonic or utilitarian values, the present study aims to understand what AR attributes create hedonic and utilitarian values and how their interaction determines consumers' behavioral intention.Design/methodology/approachThe study synthesizes the results of 19 quantitative studies on AR marketing by using the meta-analysis technique.FindingsThe findings reveal that interactivity and augmentation are salient AR attributes that offer users both hedonic and utilitarian values. They are instrumental in fostering users' behavioral intention. However, interactivity does not have any direct influence on the behavioral intentions.Originality/valueBeing one of the first meta-analyses on AR marketing; theoretically, it synthesizes the statistical data of the state of art literature on AR marketing. The results of the study would allow AR practitioners to decide on their AR marketing related activities in a better way.