bluuebunny/crossref_metadata_2025_split huggingface dataset:
DJ-1 interacts with the ectopic ATP-synthase in endothelial cells during acute ischemia and reperfusion
Authors: Gallinat Alex , Badimon Lina 
Published: July 2022

AbstractEndothelial cells (ECs) play a central role in ischemia. ATP-Synthase is now recognized to be ectopically expressed in the cell surface of many cell types, with putative roles described in angiogenesis, proliferation, and intracellular pH regulation. DJ-1 is a multifunctional protein, involved in cell protection against ischemia, ischemia–reperfusion (I/R), and oxidative stress, that regulates mitochondrial ATP-synthase. Here we focused on the characterization of the endothelial dynamics of DJ-1, and its implication in the regulation of the ectopic ATP-synthase (ecATP-S) activity, during acute ischemia and I/R in ECs. We found that DJ-1 is secreted from ECs, by a mechanism enhanced in ischemia and I/R. A cleaved form of DJ-1 (DJ-1∆C) was found only in the secretome of ischemic cells. The ecATP-S activity increased following acute ischemia in ECs, coinciding with DJ-1 and DJ-1∆C secretion. The inhibition of DJ-1 expression inhibited the ecATP-S response to ischemia by ∼ 50%, and its exogenous administration maximized the effect, together with an enhanced Akt phosphorylation and angiotube-formation potential at reperfusion. Immunoprecipitation studies showed direct interaction between DJ-1 and the ecATP-S. Altogether suggesting that DJ-1 is actively cleaved and released from ischemic ECs and plays an important role in the regulation of the ecATP-S activity during acute ischemia and reperfusion.
Structural design and performance study of primitive triply periodic minimal surfaces Ti6Al4V biomimetic scaffold
Authors: Qin Yaru , Wang Qihui , Shi Chenglong , Liu Bing , Ma Shuqing , Zhang Miao 
Published: July 2022

AbstractThis paper comprehensively evaluated the static mechanical compressive properties, permeability, and cell adhesion effect on the inner wall of the Primitive triply periodic minimal surface Ti6Al4V bionic scaffolds with different axial diameter ratios through numerical simulation and experiments. The results show that when the axial diameter ratio is 1:2, the elastic modulus of the scaffold is about 1.25 and the yield strength is about 1.36. The scaffold's longitudinal and transverse mechanical properties align with human bone tissue. Its permeability is also better than that of circular pores. The scaffold with an axial diameter ratio of 1:3 has the best permeability, ranging from 1.28e−8 to 1.60e−8 m2, which is more conducive to the adsorption of cells on the inner wall of the scaffold. These results show that the scaffold structure with an axial diameter ratio of not 1:1 has more advantages than the ordinary uniform scaffold structure with an axial diameter ratio of 1:1. This is of great significance to the optimal design of scaffold.
Reference gene selection in bovine caruncular epithelial cells under pregnancy-associated hormones exposure
Authors: Sozoniuk Magdalena , Jamioł Monika , Kankofer Marta , Kowalczyk Krzysztof 
Published: July 2022

AbstractExamination of transcriptional regulation occurring during pregnancy establishment and maintenance requires the identification of endogenous reference genes characterized by high expression stability. Since the expression of some reference genes may be modulated by pregnancy-associated hormones, the goal of our study was to identify suitable reference genes unaffected by hormonal treatment. In our study bovine caruncular epithelial cells were subjected to progesterone, estrogen and prostaglandin F2αtreatment. Ten candidate reference genes (ACTR1A, CNOT11, HDAC1, HPRT1, RPL19, RPS9, SDHA, SUZ12, UXTandZNF131) were evaluated with the use of four approaches (geNorm, NormFinder, BestKeeper, delta Ct). We found thatRPS9andSUZ12displayed the highest expression stability in the tested material. Moreover,HPRT1andSDHAwere found inappropriate for RT-qPCR data normalization as they demonstrated the highest expression variability out of all candidates analysed. Hence geNorm calculations shown that the use of just two best-performing genes would be sufficient for obtaining reliable results, we propose thatRPS9andSUZ12be used as suitable endogenous controls in future studies investigating gene expression in normal and compromised pregnancies.
The association of meteorological parameters and AirQ+ health risk assessment of PM2.5 in Ratchaburi province, Thailand
Authors: Kliengchuay Wissanupong , Srimanus Wechapraan , Srimanus Rachodbun , Kiangkoo Nuttapohn , Moonsri Kamontat , Niampradit Sarima , Suwanmanee San , Tantrakarnapa Kraichat 
Published: July 2022

AbstractAir quality is heavily influenced by rising pollution distribution levels which are a consequence of many artificial activities from numerous sources. This study aims to determine the relationship between meteorological data and air pollutants. The health effects of long-term PM2.5 were estimated on expected life remaining (ELR) and years of life lost (YLL) indices in Ratchaburi province during the years 2015–2019 using AirQ+ software. Values obtained from the PM2.5 averaging, and YLL data were processed for the whole population in the age range of 0–29, 30–60 and over 60. These values were entered into AirQ+ software. The mean annual concentration of PM2.5 was highly variable, with the highest concentration being 136.42 μg/m3 and the lowest being 2.33 μg/m3. The results estimated that the highest and lowest YLL in the next 10 years for all age groups would be 24,970.60 and 11,484.50 in 2017 and 2019, respectively. The number of deaths due to COPD, IHD, and stroke related to long-term exposure to ambient PM2.5 were 125, 27 and 26, respectively. The results showed that older people (> 64) had a higher YLL index than the groups aged under 64 years. The highest and lowest values for all ages were 307.15 (2015) and 159 (2017). Thus, this study demonstrated that the PM2.5 effect to all age groups, especially the the elderly people, which the policy level should be awared and fomulated the stratergies to protecting the sensitive group.
The green tea catechin EGCG provides proof-of-concept for a pan-coronavirus attachment inhibitor
Authors: LeBlanc Emmanuelle V. , Colpitts Che C. 
Published: July 2022

AbstractThe COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has emphasized the serious threat to human health posed by emerging coronaviruses. Effective broadly-acting antiviral countermeasures are urgently needed to prepare for future emerging CoVs, as vaccine development is not compatible with a rapid response to a newly emerging virus. The green tea catechin, epigallocatechin gallate (EGCG), has broad-spectrum antiviral activity, although its mechanisms against coronavirus (CoV) infection have remained unclear. Here, we show that EGCG prevents human and murine CoV infection and blocks the entry of lentiviral particles pseudotyped with spike proteins from bat or highly pathogenic CoVs, including SARS-CoV-2 variants of concern, in lung epithelial cells. Mechanistically, EGCG treatment reduces CoV attachment to target cell surfaces by interfering with attachment to cell-surface glycans. Heparan sulfate proteoglycans are a required attachment factor for SARS-CoV-2 and are shown here to be important in endemic HCoV-OC43 infection. We show that EGCG can compete with heparin, a heparan sulfate analog, for virion binding. Our results highlight heparan sulfate as a conserved cell attachment factor for CoVs, and demonstrate the potential for the development of pan-coronavirus attachment inhibitors, which may be useful to protect against future emerging CoVs.
Label-free viability assay using in-line holographic video microscopy
Authors: Boltyanskiy Rostislav , Odete Mary Ann , Cheong Fook Chiong , Philips Laura A. 
Published: July 2022

AbstractTotal holographic characterization (THC) is presented here as an efficient, automated, label-free method of accurately identifying cell viability. THC is a single-particle characterization technology that determines the size and index of refraction of individual particles using the Lorenz–Mie theory of light scattering. Although assessment of cell viability is a challenge in many applications, including biologics manufacturing, traditional approaches often include unreliable labeling with dyes and/or time consuming methods of manually counting cells. In this work we measured the viability of Saccharomyces cerevisiae yeast in the presence of various concentrations of isopropanol as a function of time. All THC measurements were performed in the native environment of the sample with no dilution or addition of labels. Holographic measurements were made with an in-line holographic microscope using a 40$$\times$$
×
objective lens with plane wave illumination. We compared our results with THC to manual counting of living and dead cells as distinguished with trypan blue dye. Our findings demonstrate that THC can effectively distinguish living and dead yeast cells by the index of refraction of individual cells.
STING expression is an independent prognostic factor in patients with mycosis fungoides
Authors: Takayanagi-Hara Reiko , Sawada Yu , Sugino Hitomi , Minokawa Yoko , Kawahara-Nanamori Hikaru , Itamura Misa , Tashiro Tomoko , Kaneoka Ayaka , Saito-Sasaki Natsuko , Yamamoto Kayo , Okada Etsuko 
Published: July 2022

AbstractMycosis fungoides is recognized as an indolent cutaneous malignant T-cell lymphoma. In contrast, there are few therapeutic options for advanced forms of mycosis fungoides. Since immunotherapy is desirable as an alternative therapeutic option, identifying candidate molecules is an important goal for clinicians. Although tumor-derived negative immunomodulatory molecules, such as PD-1/PD-L1, have been identified in various malignancies, the useful positive immunological drivers of mycosis fungoides are largely unknown. We found that the stimulator of interferon (IFN) genes (STING) was highly upregulated in early-stage mycosis fungoides. Immunohistochemical examination revealed different STING staining patterns in patients with mycosis fungoides. Although there were no significant differences in clinical factors’ characteristics, STING expression was associated with the survival of patients with mycosis fungoides. The survival rate was significantly poor in patients with low STING-expressing mycosis fungoides. Univariate and multivariate analyses revealed that low STING expression was associated with an increased hazard ratio. Our results indicate that STING expression independently influences the prognosis of mycosis fungoides.
National Burden and Trend of Cancer in Ethiopia, 2010–2019: a systemic analysis for Global burden of disease study
Authors: Awedew Atalel Fentahun , Asefa Zelalem , Belay Woldemariam Beka 
Published: July 2022

AbstractOver the last two decades, we have tracked the national burden of cancer and its trends in Ethiopia, providing estimates of incidence, death, and disability adjusted life years. In Ethiopia, there were an estimated 53,560 (95% UI 52,480–55,540) new incident cases, 39,480 deaths (95% UI 32,640–46,440), and 1.42 million (95% UI 1.16–1.68) DALYs of cancer 2019. Cancer incidence, death, and DALYs counts increased by 32% (95% UI 11–55%), 29% (95% UI 12–44%), and 19% (95% UI − 2 to 44%) between 2010 to 2019, respectively, while age-standardised incidence, death, and DALYs rates increased by 5% (95% UI − 7 to 18%), 2% (95% UI − 9 to 14%), and − 2% (95% UI − 15 to 12%) respectively. In 2019, the leading incidence cases were leukemia, cervical cancer, breast cancer, colon and rectum cancer, and stomach cancer, while leukemia, breast cancer, cervical cancer, and stomach cancer were the most common killer cancers in Ethiopia. According to the findings of this study, tobacco-related cancers such as pancreatic, kidney, and lung cancer have increased in Ethiopian females over the last decade, while genitourinary cancer has increased in Ethiopian males. Another significant finding was that infection-related cancers, such as stomach cancer and Hodgkin lymphoma, have been rapidly declining over the last decade.
Accurate automatic object 4D tracking in digital in-line holographic microscopy based on computationally rendered dark fields
Authors: Rogalski Mikołaj , Picazo-Bueno Jose Angel , Winnik Julianna , Zdańkowski Piotr , Micó Vicente , Trusiak Maciej 
Published: July 2022

AbstractBuilding on Gabor seminal principle, digital in-line holographic microscopy provides efficient means for space–time investigations of large volumes of interest. Thus, it has a pivotal impact on particle tracking that is crucial in advancing various branches of science and technology, e.g., microfluidics and biophysical processes examination (cell motility, migration, interplay etc.). Well-established algorithms often rely on heavily regularized inverse problem modelling and encounter limitations in terms of tracking accuracy, hologram signal-to-noise ratio, accessible object volume, particle concentration and computational burden. This work demonstrates the DarkTrack algorithm—a new approach to versatile, fast, precise, and robust 4D holographic tracking based on deterministic computationally rendered high-contrast dark fields. Its unique capabilities are quantitatively corroborated employing a novel numerical engine for simulating Gabor holographic recording of time-variant volumes filled with predefined dynamic particles. Our solution accounts for multiple scattering and thus it is poised to secure an important gap in holographic particle tracking technology and allow for ground-truth-driven benchmarking and quantitative assessment of tracking algorithms. Proof-of-concept experimental evaluation of DarkTrack is presented via analyzing live spermatozoa. Software supporting both novel numerical holographic engine and DarkTrack algorithm is made open access, which opens new possibilities and sets the stage for democratization of robust holographic 4D particle examination.
Weak effects on growth and cannibalism under fluctuating temperatures in damselfly larvae
Authors: Holzmann Kim Lea , Charrier Chloé , Johansson Frank 
Published: July 2022

AbstractThe Earth’s climate is changing with a trend towards higher mean temperatures and increased temperature fluctuations. Little attention has been paid to the effects of thermal variation on competition within species. Understanding the temperature-dependence of competition is important since it might affect dynamics within and between populations. In a laboratory experiment we investigated the effects of thermal variation on growth and cannibalism in larvae of a damselfly. The temperature treatments included three amplitudes between 20 and 26 °C with an average of 23 °C, and a constant control at 23 °C. Larvae were also raised at five constant temperatures for an estimation of the thermal performance curve, which showed that the thermal optimum for growth was 26.9 °C. Cannibalism was significantly positively correlated with initial body size variance. There was neither a difference among the temperature variation treatments, nor between the constant and the variation treatments in growth and cannibalism. Hence, positive and negative effects of temperature variation within the linear range of a species thermal performance curve might cancel each other out. Since our study mimicked natural temperature conditions, we suggest that the increase in temperature variation predicted by climate models will not necessarily differ from the effects without an increase in variation.
Monochromatic light measurement via geometric phase and Fourier-transform spectroscopy method
Authors: Garoi Florin , Nicolae Ionut , Prepelita Petronela 
Published: July 2022

AbstractThe paper introduces a method for the measurement of monochromatic light using the geometric phase shift in a polarizing interferometer and applying the Fourier transform spectroscopy method. This is achieved with no mirror displacement or change in the actual optical path difference. Our method uses the rotation of a half-wave plate with increments on the order of degrees of arc, which is easier to control and reliable to reproduce. This approach provides flexibility in choosing the spectral range and a novel way of performing such measurements. It is demonstrated how the achromatic nature of the geometric phase allows only for monochromatic measurements to be acquired. The method is described theoretically and its performance is validated via measurements of several low-coherence light sources. Three possible applications of the method are also described, two of which are advantaged by using a detector array and, surprisingly, the achromaticity of the geometric phase.
Ab-initio study of pressure influenced elastic, mechanical and optoelectronic properties of Cd0.25Zn0.75Se alloy for space photovoltaics
Authors: Iqbal Muhammad Aamir , Malik Maria , Shahid Wajeehah , Irfan Shaheen , Alguno Arnold C. , Morsy Kareem , Capangpangan Rey Y. , Pham Phuong V. , Choi Jeong Ryeol 
Published: July 2022

AbstractThe optoelectronic properties of the ternary Cd0.25Zn0.75Se alloy are reported under the influence of a high pressure ranging from 0 to 25 GPa, within a modified Becke–Jhonson potential using density functional theory. This alloy has a cubic symmetry, is mechanically stable, and its bulk modulus rises with pressure. It is observed to be a direct bandgap material with a bandgap energy that increases from 2.37 to 3.11 eV with rise in pressure. Pressure changes the optical and electronic properties, causing the absorption coefficient to rise and absorb visible green-to-violet light. The static dielectric constant, along with the static index of refraction, both increase under the influence of pressure. Optical constants, including dielectric constant, optical conductivity, refractive index, extinction coefficient, and reflection, are also investigated and discussed. This DFT forecast explores important research directions for the usage of the CdZnSe semiconductor alloys in the manufacturing of space photovoltaic and optoelectronic devices operating at different pressures.
Leg dominance and performance in change of directions tests in young soccer players
Authors: Clemente Filipe Manuel , González-Fernández Francisco Tomás , García-Delgado Gabriel , Silva Rui , Silva Ana Filipa , Nobari Hadi , Falces-Prieto Moisés 
Published: July 2022

AbstractThe present study aimed to examine the influence of leg dominance on the change of direction (COD) performance. In this study participated 94 healthy young highly trained male soccer players belonging from two categories (n = 27 vs. n = 67; 14.81 ± 0.40 vs. 16.64 ± 1.25 years of age; 170.61 ± 5.61 vs. 173.73 ± 7.19 cm of height; 64.74 ± 8.44 vs. 66.70 ± 7.95 kg of weight, for U16 and U18, respectively). Fitness assessments were performed two times in a period of three months, and included: (1) anthropometry measures, (2) 30–15 IFT, (3) 10-m sprint test, (4) 505-COD test, 90° COD test and cross-over hop test. A paired sample t-test was performed to evaluate the asymmetries at the intragroup level in each of the COD’s tests. A symmetry index was used to analyse the asymmetries between categories, and an independent sample t-test was used to compare the variability between the two categories in each of the three tests performed. The effect size was also evaluated. Analysis demonstrated that evidence a trend for a better performance with the preferred leg in the cross-over hop and 505-COD tests, and with the non-dominant leg in the 90° COD. However, in the intragroup analysis, only the 505-COD test registered differences, and no differences were notice din the intergroup comparison. Only in the 505-COD test the percentage of variability (CV) was statistically significant (7.03 ± 4.18% vs. 4.03 ± 2.02% from U16 and U18, respectively). In sum, bilateral differences were noticed in the intragroup comparison, although only in 505-COD test the leg dominance showed to influence performance. In the intergroup analysis any difference was noticed between age categories.
Quantum channel measurement with local quantum Bernoulli noises
Authors: Han Qi , Han Yanan , Kou Yaxin , Bai Ning 
Published: July 2022

AbstractAs an important stochastic process, quantum Bernoulli noises has a very important physical background and is an important research object in the field of quantum information. In this paper, we review local quantum Bernoulli noises and local quantum mutual entropy, then introduce quantum channel measurement with local quantum Bernoulli noises. On this basis, we give the channel structure between two systems, and prove the completely positivity of this quantum channel. We also give a channel application on the local quantum mutual entropy.
Effect of calcination on coarse gangue rejection of hard rock lithium ores
Authors: Nazir Muhammad Kashif , Dyer Laurence , Tadesse Bogale , Albijanic Boris , Kashif Nadia 
Published: July 2022

AbstractProcessing of spodumene ores requires calcination as a compulsory pre-treatment to convert α-spodumene to a more reactive β-spodumene phase. This transformation takes place at an elevated temperature of above 900 °C and results in a 30% volumetric expansion of the mineral and the product having highly altered physical properties. This work examines these induced properties and the effect of calcination on lithium grade deportment with particle size. XRD analysis showed a significant amount of β-spodumene in the calcined finest fraction (i.e. the particles less than 0.6 mm). A marked reduction in the bond ball mill work index of the calcined lithium samples (i.e. 42.3%) was recorded supporting the observed fracturing and friable appearance of the sample following α to β-spodumene conversion. The deportment of lithium to finer fractions was significantly increased when the sample was calcined, indicating selective breakage of the spodumene over gangue minerals.
The CCR5 antagonist maraviroc exerts limited neuroprotection without improving neurofunctional outcome in experimental pneumococcal meningitis
Authors: Le Ngoc Dung , Steinfort Marel , Grandgirard Denis , Maleska Aleksandra , Leppert David , Kuhle Jens , Leib Stephen L. 
Published: July 2022

AbstractOne-third of pneumococcal meningitis (PM) survivors suffer from neurological sequelae including learning disabilities and hearing loss due to excessive neuroinflammation. There is a lack of efficacious compounds for adjuvant therapy to control this long-term consequence of PM. One hallmark is the recruitment of leukocytes to the brain to combat the bacterial spread. However, this process induces excessive inflammation, causing neuronal injury. Maraviroc (MVC)—a CCR5 antagonist—was demonstrated to inhibit leukocyte recruitment and attenuate neuroinflammation in several inflammatory diseases. Here, we show that in vitro, MVC decreased nitric oxide production in astroglial cells upon pneumococcal stimulation. In vivo, infant Wistar rats were infected with 1 × 104 CFU/ml S. pneumoniae and randomized for treatment with ceftriaxone plus MVC (100 mg/kg) or ceftriaxone monotherapy. During the acute phase, neuroinflammation in the CSF was measured and histopathological analyses were performed to determine neuronal injury. Long-term neurofunctional outcome (learning/memory and hearing capacity) after PM was assessed. MVC treatment reduced hippocampal cell apoptosis but did not affect CSF neuroinflammation and the neurofunctional outcome after PM. We conclude that MVC treatment only exerted limited effect on the pathophysiology of PM and is, therefore, not sufficiently beneficial in this experimental paradigm of PM.