bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fine-Scale Climate Projections: What Additional Fixed Spatial Detail Is Provided by a Convection-Permitting Model?
Authors: Rowell David P. , Berthou Ségolène 
Published: November 2022

Abstract
Convection-permitting (CP) models promise much in response to the demand for increased localization of future climate information: greater resolution of influential land surface characteristics, improved representation of convective storms, and unprecedented resolution of user-relevant data. In practice, however, it is contended that the benefits of enhanced resolution cannot be fully realized due to the gap between models’ computational and effective resolution. Nevertheless, where surface forcing is strongly heterogeneous, one can argue that usable information may persist close to the grid scale. Here we analyze a 4.5-km resolution CP projection for Africa, asking whether and where fine-scale projection detail is robust at sub-25-km scales, focusing on geolocated rainfall features (rather than Lagrangian motion). Statistically significant detail for seasonal means and daily extremes is most frequent in regions of high topographic variability, most prominently in East Africa throughout the annual cycle, West Africa in the monsoon season, and to a lesser extent over Southern Africa. Lake coastal features have smaller but significant impacts on projection detail, whereas ocean coastlines and urban conurbations have little or no detectable impact. The amplitude of this sub-25-km projection detail can be similar to that of the local climatology in mountainous regions (or around a third near East Africa’s lake shores), so potentially beneficial for improved localization of future climate information. In flatter regions distant from coasts (the majority of Africa), spatial heterogeneity can be explained by chaotic weather variability. Here, the robustness of local climate projection information can be substantially enhanced by spatial aggregation to approximately 25-km scales, especially for daily extremes and equatorial regions.

Significance Statement
Recent substantial increases in the horizontal resolution of climate models bring the potential for both more reliable and more local future climate information. However, the best spatial scale on which to analyze such data for impacts assessments remains unclear. We examine a 4.5-km resolution climate projection for Africa, focusing on seasonal and daily rainfall. Spatially fixed fine-scale projection detail is found to be statistically robust at sub-25-km scales in the most mountainous regions and to a lesser extent along lake coastlines. Elsewhere, the model data may be better aggregated to at least 25-km scales to reduce sampling uncertainties. Such evolving guidance on the circumstances and extent of high-resolution data aggregation will help users gain greater benefit from climate model projections.
Impact of the coronavirus disease 2019 pandemic on ultrasound screening of developmental dysplasia of the hip and follow-up imaging: a report from southeastern Turkey
Authors: Ayaz Ercan 
Published: November 2022

Objective To investigate the effect of coronavirus disease 2019 restrictions on ultrasound (US) screening of developmental dysplasia of the hip (DDH) in a children’s hospital. Methods The records of US screening of DDH were retrospectively evaluated in the pandemic period (April 2020 to July 2021) and the pre-pandemic period (January 2019 to February 2020). The monthly US number, sex, radiologist number, and age at the initial examination were recorded. Results A total of 6107 US scans were performed during the pre-pandemic period, which significantly decreased to 3340 during the pandemic. The number of monthly US scans performed did not change between the pre-pandemic (142.7/month) and pandemic (128.2/month) periods. The number of delayed examinations in the total population did not significantly change between the two periods. However, the number of delayed examinations in patients with abnormal hips was significantly increased during the pandemic compared with that in the pre-pandemic period. Conclusions Coronavirus disease 2019 restrictions decreased the US screening rate of DDH by almost half, but the number of US scans performed by each radiologist was unchanged. The compliance with follow-up recommendations was reduced by half, which may lead to an increase in the incidence of delayed and untreated DDH cases.
He kāinga pai rawa: Te Kete Mātauranga mō te hanga whare tino pai rawa: a knowledge basket to support building affordable and safe housing for kaumātua
Authors: Nock Sophie , Wilson Yvonne , Reddy Rangimahora , Holmes Kath , Simpson Mary , Oetzel John 
Published: November 2022

Tuhinga whakarāpopoto (Abstract) Poor, unaffordable and overcrowded housing among Māori in Aotearoa New Zealand is one of the converging societal trends to impact significantly on older Māori ageing in place and in age-friendly environments. Some kaupapa Māori (Māori approach) organisations have sought to develop kaumātua (elders) villages to address these challenges. From the study of one such village, a toolkit of successful practices was developed. The purpose of this article is to describe the research design and methods for a project that will use this toolkit to develop community determined villages in three additional communities. The research approach involves process evaluation using photovoice, interviews, wānanga (consultation meetings) seminars and meeting’s notes, along with summative evaluation using surveys. The research process is grounded in a culture-centred and co-design approach with a vision underpinned by tikanga Māori (Māori custom) and te ao Māori (Māori world) that will be shared with others through a revised toolkit.
Engineered and banked iPSCs for advanced NK- and T-cell immunotherapies
Authors: Cichocki Frank , van der Stegen Sjoukje J. C. , Miller Jeffrey S. 
Published: November 2022

Abstract
The development of methods to derive induced pluripotent stem cells (iPSCs) has propelled stem cell research, and has the potential to revolutionize many areas of medicine, including cancer immunotherapy. These cells can be propagated limitlessly and can differentiate into nearly any specialized cell type. The ability to perform precise multigene engineering at the iPSC stage, generate master cell lines after clonal selection, and faithfully promote differentiation along natural killer (NK) cells and T-cell lineages is now leading to new opportunities for the administration of off-the-shelf cytotoxic lymphocytes with direct antigen targeting to treat patients with relapsed/refractory cancer. In this review, we highlight the recent progress in iPSC editing and guided differentiation in the development of NK- and T-cell products for immunotherapy. We also discuss some of the potential barriers that remain in unleashing the full potential of iPSC-derived cytotoxic effector cells in the adoptive transfer setting, and how some of these limitations may be overcome through gene editing.
Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice
Authors: Chen Weilan , Hu Xiaoling , Hu Li , Hou Xinyue , Xu Zhengyan , Yang Fanmin , Yuan Min , Chen Feifan , Wang Yunxiao , Tu Bin , Li Ting , Kang Liangzhu , Tang Shiwen , Ma Bingtian , Wang Yuping , Li Shigui , Qin Peng , Yuan Hua 
Published: November 2022

Abstract
Background:
Grain size is a direct determinant of grain weight and yield in rice; however, the genetic and molecular mechanisms determining grain size remain largely unknown.

Findings:
We identified a mutant, wide grain 3 (wg3), which exhibited significantly increased grain width and 1000-grain weight. Cytological analysis showed that WG3 regulates grain size by affecting cell proliferation. MutMap-based gene cloning and a transgenic experiment demonstrated that WG3 encodes a GRAS protein. Moreover, we found that WG3 directly interacts with DWARF AND LOW-TILLERING (DLT), a previously reported GRAS protein, and a genetic experiment demonstrated that WG3 and DLT function in a common pathway to regulate grain size. Additionally, a brassinosteroid (BR) sensitivity test suggested that WG3 has a positive role in BR signaling in rice. Collectively, our results reveal a new genetic and molecular mechanism for the regulation of grain size in rice by the WG3-DLT complex, and highlight the important functions of the GRAS protein complex in plants.

Conclusion:
WG3 functions directly in regulating grain size and BR signaling in rice.
Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy
Authors: Sun Kai , Li Dandan , Xia Aoyun , Zhao Hua , Wen Qin , Jia Sisi , Wang Jiafeng , Yang Guili , Zhou Danhua , Huang Cuihong , Wang Hui , Chen Zhiqiang , Guo Tao 
Published: November 2022

Abstract
Background
The mining of new allelic variation and the induction of new genetic variability are the basis for improving breeding efficiency.

Results
In this study, in total, 3872 heavy ion-irradiated M2 generation rice seeds and individual leaves were collected. The grain length was between 8 and 10.22 mm. The grain width was between 1.54 and 2.87 mm. The results showed that there was extensive variation in granulotype. The allelic variation in GS3 and GW5 was detected in 484 mixed samples (8:1) using targeted sequencing technology, and 12 mixed samples containing potential mutations and 15 SNPs were obtained; combined with Sanger sequencing and phenotype data, 13 key mutants and their corresponding SNPs were obtained; protein structural and functional analysis of key mutants screened out 6 allelic variants leading to altered grain shape, as well as the corresponding mutants, including long-grain mutants GS3-2 and GS3-7, short-grain mutants GS3-3 and GS3-5, wide-grain mutant GW5-1 and narrow-grain mutant GW5-4; whole genome sequencing identified new grain length gene allelic variants GS3-G1, GS3-G2 and GS3-G3.

Conclusion
Based on the above studies, we found 6 granulotype mutants and 9 granulotype-related allelic variants, which provided new functional gene loci and a material basis for molecular breeding and genotype mutation and phenotype analysis. We propose a method for targeted identification of allelic variation in rice grain type genes by combining targeted sequencing of mixed samples and whole genome sequencing. The method has the characteristics of low detection cost, short detection period, and flexible detection of traits and genes.
Retrospective analysis of SARS-CoV-2 omicron invasion over delta in French regions in 2021–22: a status-based multi-variant model
Authors: Haschka Thomas , Vergu Elisabeta , Roche Benjamin , Poletto Chiara , Opatowski Lulla 
Published: November 2022

Abstract
Background
SARS-CoV-2 is a rapidly spreading disease affecting human life and the economy on a global scale. The disease has caused so far more then 5.5 million deaths. The omicron outbreak that emerged in Botswana in the south of Africa spread around the globe at further increased rates, and caused unprecedented SARS-CoV-2 infection incidences in several countries. At the start of December 2021 the first omicron cases were reported in France.

Methods
In this paper we investigate the spreading potential of this novel variant relatively to the delta variant that was also in circulation in France at that time. Using a dynamic multi-variant model accounting for cross-immunity through a status-based approach, we analyze screening data reported by Santé Publique France over 13 metropolitan French regions between 1st of December 2021 and the 30th of January 2022. During the investigated period, the delta variant was replaced by omicron in all metropolitan regions in approximately three weeks. The analysis conducted retrospectively allows us to consider the whole replacement time window and compare regions with different times of omicron introduction and baseline levels of variants’ transmission potential. As large uncertainties regarding cross-immunity among variants persist, uncertainty analyses were carried out to assess its impact on our estimations.

Results
Assuming that 80% of the population was immunized against delta, a cross delta/omicron cross-immunity of 25% and an omicron generation time of 3.5 days, the relative strength of omicron to delta, expressed as the ratio of their respective reproduction rates, $$\frac{\hat{R}_{\textrm{omicron}}}{\hat{R}_{\textrm{delta}}}$$

R
^

omicron

R
^

delta

, was found to range between 1.51 and 1.86 across regions. Uncertainty analysis on epidemiological parameters led to $$\frac{\hat{R}_{\textrm{omicron}}}{\hat{R}_{\textrm{delta}}}$$

R
^

omicron

R
^

delta

ranging from 1.57 to 2.34 on average over the metropolitan French regions, weighted by population size.

Conclusions
Upon introduction, omicron spread rapidly through the French territory and showed a high fitness relative to delta. We documented considerable geographical heterogeneities on the spreading dynamics. The historical reconstruction of variant emergence dynamics provide valuable ground knowledge to face future variant emergence events.
Cone-beam computed tomography is not a mandatory procedure in adrenal venous sampling for primary hyperaldosteronism
Authors: Cai Ran , Hu Chao , Li Hai-Yang 
Published: November 2022

Abstract
Objectives
To investigate the necessity of cone-beam computed tomography (CBCT) in adrenal venous sampling (AVS).

Methods
This retrospective study included 120 consecutive patients with primary hyperaldosteronism who underwent AVS. Based on the learning curve of the interventional radiologists, the patients were divided into the learning (n = 36) and proficiency (n = 84) groups chronologically. Based on the imaging pattern of the right adrenal vein (RAV), the patients were divided into the typical (n = 36) and atypical (n = 84) groups. The success rate, radiation dose, and sampling time were compared among the entire study population and each subgroup.

Results
A total of 69 patients underwent CBCT, whereas 51 patients did not. The overall success rate was 85.8%, and no difference was noted between patients with and without CBCT (P = 0.347). However, radiation dose (P = 0.018) and sampling time (P = 0.001) were significantly higher in patients who underwent CBCT than in patients who did not. In learning group, CBCT improved success rate from 62.5 to 96.4% (P = 0.028), whereas it was not found in the proficiency group (P = 0.693). Additionally, success rate in patients with an atypical RAV imaging pattern was significantly higher when CBCT was used than when it was not used (P = 0.041), whereas no difference was noted in patients with typical RAV imaging pattern (P = 0.511).

Conclusion
For physicians not very experienced doing AVS, there is a clear significant improvement in success rate when CBCT is used. However, CBCT only has minimal benefit for experienced operators, meanwhile CBCT may take an extra time and increase the radiation dose during AVS.
Identification of key cuproptosis-related genes and their targets in patients with IgAN
Authors: Lin Huagang , Wu Deping , Xiao Jing 
Published: November 2022

Abstract
Background
IgA nephropathy (IgAN) is one of the most common forms of chronic glomerulonephritis, but the aetiology and pathogenesis remain unclear. Cuproptosis is a newly identified form of cell death that plays an important role in many diseases. Researchers have not clearly determined whether the expression of cuproptosis-related genes (CRGs) is involved in the pathogenesis of IgAN.

Methods
The GSE93798, GSE50469 and GSE37460 datasets containing microarray data from patients with IgAN (63) and healthy controls (31) were downloaded from the GEO database. Immune cells and immune-related functions were analysed in patients with IgAN and controls, and genes were identified that may be related to cuproptosis. A logistic regression model was established according to the results, and then GO and KEGG enrichment analyses were performed. Finally, possible drugs were selected using the DSigDB database.

Results
The subjects in the different groups showed significantly different fractions of immune cells and immune-related functions, and 11 genes related to cuproptosis may be involved in these processes. Based on these 11 genes, the ROC curve was plotted, and the AUC value was calculated (0.898, 95% CI: 0.839–0.958). The result revealed good predictability. Then, genes with P < 0.05 (lipoyltransferase 1, LIPT1) were selected to plot an ROC curve, and the AUC value was calculated (0.729, 95% CI: 0.636–0.821). Enrichment analyses showed that the TCA cycle and multiple metabolic pathways may also be involved in the occurrence of IgAN. Finally, 293 potential drugs that may be used to treat IgAN were identified based on these genes.

Conclusion
In this study, we identified some novel CRGs that may be involved in IgAN, among which LIPT1 was significantly differentially expressed. It may predict the risk of IgAN and provides a possible target for the treatment of IgAN. Further experimental studies are needed to explore how these CRGs mediate the occurrence and development of IgAN.
The effect of vitreomacular interface in neovascular age-related macular degeneration treated with intravitreal injection of anti-VEGF
Authors: Han Fangyuan , Chen Xingwang , Zhao Ruyi , Jin Xin , Tan Wei , Zhang Ying 
Published: November 2022

Abstract
Background
The purpose of this study is to study the effect of repeated intravitreal injection of anti-vascular endothelial growth factor (anti-VEGF) drugs on vitreomacular interface.

Methods
Neovascular age-related macular degeneration patients who received intravitreal injections of anti-VEGF drugs were included. Eyes with severe vitreous opacity, uveitis, complicated cataract surgery and previous vitrectomy were excluded. Vitreomacular interface, best corrected visual acuity (BCVA) and central retinal thickness (CRT) assessment were performed once a month for at least 3 months. The nature and time of the change event are recorded. Groups were divided according to whether vitreomacular interface change events occurred. To analyse the risk factors of vitreomacular interface changes and their influence on treatment effect.

Results
A total of 87 eyes were evaluated. Vitreomacular interface change event occurred in 9 eyes. Pre-existing vitreomacular interface abnormality (VMIA) was a risk factor for the VMI change (P = 0.033, OR = 16.518, 95% CI: 1.258 to 216.939). 60% of interface events occurred in the first 3 months of treatment. The final BCVA of eyes with vitreomacular interface unchanged was significantly higher than that at baseline (P = 0.001), and the final CRT was also significantly lower than that at baseline (P < 0.001). The final CRT of eyes vitreomacular interface changed was significantly lower than that at baseline (P = 0.015), however, there was no statistical significance in BCVA (P = 0.468).

Conclusion
Intravitreal injection of anti-VEGF drugs has a certain probability to cause changes in the vitreomacular interface, and the risk is higher in eyes with pre-existing vitreomacular interface abnormality. The effect of intravitreal injections on the vitreomacular interface was concentrated in the first three injections, and subsequent increases in the number of injections did not significantly increase the risk of vitreomacular interface abnormality. Ophthalmologists should increase attention to the vitreomacular interface in the early stages of anti-VEGF therapy and counsel patients accordingly.
Accuracy of the spontaneous breathing trial using a combined CPAP + PSV model to predict extubation outcomes in very preterm infants
Authors: Li Zhe , Xue Jiang , Guo Xin-Yuan , Wang Fang , Zhang Xue , Li Qi , Tang Jing-Liang , Ji Juan , Du Guang-Jun 
Published: November 2022

Abstract
Background
Very preterm infants often require mechanical ventilation. However, objective criteria to predict the outcomes of extubation in very premature neonates remain lacking. The aim of this study was to investigate the accuracy of the spontaneous breathing trial (SBT) using a combined model of continuous positive airway pressure (CPAP) and low-level pressure support ventilation (PSV) to predict the extubation outcomes of preterm infants with gestational age < 32 weeks.

Methods
Preterm infants with gestational age < 32 weeks, birth weight < 1500 g and requiring mechanical ventilation were selected for the study. All infants underwent a 10-minute SBT using CPAP combined with low-level PSV prior to the planned extubation. Then, the infants were extubated within 1 h after SBT. The outcomes of extubation were considered successful if the neonates did not require reintubation 72 h after extubation.

Results
A total of 119 eligible preterm infants were enrolled in the study, with a median gestational age of 28.9 (27.1–30.3) weeks and a median birth weight of 1100 (900–1350) g. In total, 101 of all infants had successful extubation, 18 of whom failed and eventually had to be reintubated. Of the 102 infants who achieved SBT, 99 were successfully extubated, and 15 of the 17 infants who did not pass SBT had failed extubation. Finally, the diagnostic value for SBT could be assessed with a sensitivity of 98%, a specificity of 83.3%, a positive predictive value of 97.1% and a negative predictive value of 88.2%.

Conclusion
SBT using a combined CPAP + low-level PSV model can predict the outcomes of extubation in very preterm infants with high sensitivity and specificity.
Effect of oral motor facilitation technique on oral motor and feeding skills in children with cerebral palsy : a case study
Authors: Min Kyoung-chul , Seo Sang-min , Woo Hee-soon 
Published: November 2022

Abstract
Background:
Deficiencies in oral motor function and feeding skills are common in children with cerebral palsy (CP). Oral motor therapy is a useful method to improve oral motor function and feeding skills. Oral motor facilitation technique (OMFT) is a newly designed comprehensive oral motor therapy, including postural control, sensory adaptation, breathing control, sensorimotor facilitation, and direct feeding.

Methods:
This study was performed to identify the effect of OMFT on oral motor function and feeding skills in children with CP. A total of 21 children with CP (3–10 years, GMFCS III–V) participated in 16 weeks (16 sessions) of OMFT. The effects on oral motor function and feeding skills were assessed using the Oral Motor Assessment Scale (OMAS) before the treatment, 8 and 16 weeks after OMFT. Data were analyzed using the Friedman test and post-hoc analysis.

Results:
Significant improvement was found in oral motor function and feeding skills including mouth closure, lip closure on the utensil, lip closure during deglutition, control of the food during swallowing, mastication, straw suction, and control of liquid during deglutition after OMFT. Mouth closure was the most effective and mastication was the least effective item. Sixteen weeks is more effective than 8 weeks of OMFT.

Conclusion:
OMFT could be an effective and useful oral motor therapy protocol to improve oral motor function and feeding skills in children with CP.