bluuebunny/crossref_metadata_2025_split huggingface dataset:
Staphylococcal saoABC Operon Codes for a DNA-Binding Protein SaoC Implicated in the Response to Nutrient Deficit
Authors: Bukowski Michal , Kosecka-Strojek Maja , Madry Anna , Zagorski-Przybylo Rafal , Zadlo Tomasz , Gawron Katarzyna , Wladyka Benedykt 
Published: June 2022

Whilst a large number of regulatory mechanisms for gene expression have been characterised to date, transcription regulation in bacteria still remains an open subject. In clinically relevant and opportunistic pathogens, such as Staphylococcus aureus, transcription regulation is of great importance for host-pathogen interactions. In our study we investigated an operon, exclusive to staphylococci, that we name saoABC. We showed that SaoC binds to a conserved sequence motif present upstream of the saoC gene, which likely provides a negative feedback loop. We have also demonstrated that S. aureus ΔsaoB and ΔsaoC mutants display altered growth dynamics in non-optimal media; ΔsaoC exhibits decreased intracellular survival in human dermal fibroblasts, whereas ΔsaoB produces an elevated number of persisters, which is also elicited by inducible production of SaoC in ΔsaoBΔsaoC double mutant. Moreover, we have observed changes in the expression of saoABC operon genes during either depletion of the preferential carbon or the amino acid source as well as during acidification. Comparative RNA-Seq of the wild type and ΔsaoC mutant demonstrated that SaoC influences transcription of genes involved in amino acid transport and metabolism, and notably of those coding for virulence factors. Our results suggest compellingly that saoABC operon codes for a DNA-binding protein SaoC, a novel staphylococcal transcription factor, and its antagonist SaoB. We linked SaoC to the response to nutrient deficiency, a stress that has a great impact on host-pathogen interactions. That impact manifests in SaoC influence on persister formation and survival during internalisation to host cells, as well as on the expression of genes of virulence factors that may potentially result in profound alternations in the pathogenic phenotype. Investigation of such novel regulatory mechanisms is crucial for our understanding of the dynamics of interactions between pathogenic bacteria and host cells, particularly in the case of clinically relevant, opportunistic pathogens such as Staphylococcus aureus.
Comparative Research on Metabolites of Different Species of Epichloë Endophytes and Their Host Achnatherum sibiricum
Authors: Deng Yongkang , Gao Yuan , Li Chenxi , Zhang Junzhen , Fan Xiaowen , Zhao Nianxi , Gao Yubao , Ren Anzhi 
Published: June 2022

Achnatherum sibiricum can be infected by two species of fungal endophytes, Epichloë gansuensis (Eg) and Epichloë sibirica (Es). In this study, the metabolites of Eg, Es, and their infected plants were studied by GC–MS analysis. The results showed that the metabolic profiles of Eg and Es were similar in general, and only six differential metabolites were detected. The direct effect of endophyte infection on the metabolites in A. sibiricum was that endophyte-infected plants could produce mannitol, which was not present in uninfected plants. Epichloë infection indirectly caused an increase in the soluble sugars in A. sibiricum related to growth and metabolites related to the defense against pathogens and herbivores, such as α-tocopherol, α-linolenic acid and aromatic amino acids. Epichloë infection could regulate galactose metabolism, starch and sucrose metabolism, tyrosine metabolism and phenylalanine metabolism of host grass. In addition, there was a significant positive correlation in the metabolite contents between the endophyte and the host.
Design of a Piezoelectric Pump Driven by Inertial Force of Vibrator Supported by a Slotted Beam
Authors: Zhang Yong , Wang Xiaoliang , Liu Shanlin , Pan Qiaosheng 
Published: June 2022

This paper introduces the design, manufacture, dynamic analysis, and experimental results of a piezoelectric pump driven by the inertial force of a vibrator supported by a slotted beam. The piezoelectric vibrator is composed of a mass block, displacement amplifier, and slotted beam fixed with both ends. In the resonant mode, the displacement amplifier drives the slotted beam to work, and produces amplitude and inertial force. In this paper, the design of the slotted beam optimizes the output of the displacement amplifier. In addition, the slotted beam supports the displacement amplifier and increases the elastic output. The pump body adopts polydimethylsiloxane (PDMS) check valves and compressible spaces to improve the output performance. This research studies the influence of stiffness and mass on the output performance by qualitatively analyzing the inertial output force of the vibrator. Nine kinds of slotted beams with different stiffnesses and different mass blocks are designed for comparison. Thereafter, an optimal structure of the piezoelectric pump is selected. The experimental results show that under a driving voltage of 700 Vpp , the maximum flowrate is 441 mL min−1 and the maximum back pressure is 25.3 kPa.
Results of protease inhibitor instructions in patients in acute burning disease
Authors: Lynnyk O. M. , Kozynets H. P. , Osadcha O. I. 
Published: June 2022

The purpose of the study was to determine the clinical efficiency of proteinase inhibitor usage in patients with burns. Materials and Methods. The results of studies performed in 32 patients with superficial and deep burns were analyzed: 12 patients of the main group, whom were used the proteinase inhibitor drug in the complex treatment, and 20 comparison groups, who were treated according to the standard scheme. The content of calpains, α-1 proteinase inhibitor (α-1-IP), α-2 macroglobulin (α-2-MG) and tumor necrosis factor α (TNF-α) in patients’ peripheral blood were determined. Results. The decrease in proteolytic activity of blood in patients of the main group was determined as a decrease in calpain levels by 1.16 times (50.51 ± 10.32 μEq/min on the 10th-16th day compared to baseline 58.83 ± 3.62 μEq/min on the 1-4th day after injury), while in the comparison group indicators of calpain content did not tend to decrease. Comparing the TNF-α values ​​of the main group and the comparison group, it was found that the values ​​of the main group were significantly lower 1.15 times on the 5th-9th day (245.33 ± 10.36 pg/ml and 281.2 ± 13, 67 pg/ml) and 1.08 times lower on the 10th-16th day (236.60 ± 8.78 pg/ml and 256.35 ± 15.70 pg/ml). The usage of ulinastatin led to a less pronounced decreasing in proteinase inhibitors levels: α-1-IP (79.31 ± 1.54 μmol/l of the main group compared with 72.1 ± 7.8 μmol/l of the control group on the 5th-9th day after injury and 75.34 ± 5.13 μmol/l of the main group compared with 68.0 ± 4.9 μmol/l of the control group on the 10-16th day after injury), α-2-MG (2.63 ± 0.24 g/l of the main group compared with 2.2 ± 0.4 g/l of the control group on the 10-16th day after injury). Conclusion. The results of the study indicate that the proteinase inhibitor drug included in the standard treatment regimen for acute burns optimizes the proteolytic activity of peripheral blood, promotes the inflammatory response optimal development, inhibits the endothelial dysfunction development.
Aspects of quality control over the organization and examination of temporary disability at the current stage
Authors: Lashkul Z. V. , Chemyrysov V. V. , Kurochka V. L. , Motovitsa N. Ya. , Sizintsova Yu. Yu. 
Published: June 2022

Purpose of the study. Coverage of the problem and formation of recommendations for quality control over the organization and conduct of examination of temporary disability at the present stage.
Materials and methods of research. In order to highlight the problems of legal assessment of the quality of the organization and conduct an interim examination at the current level, we analyzed the regulations adopted at the level of the Cabinet of Ministers, the Ministry of Health of Ukraine, identified gaps and recommendations for adopting orders of the Ministry of Health of Ukraine. quality at the level of health care institutions
Conclusions. When implementing quality control measures for the organization and examination of temporary disability at the present stage, special attention should be paid to the development of more detailed and specific legislative documents highlighting the functional responsibilities, rights and responsibilities of health care workers, as well as internal orders governing the examination of temporary disability. disability (ETN) and all components of its activities, including the availability of quarterly and annual reports, analysis of morbidity with temporary disability, indicating specific measures to reduce it, etc. Changes in the regulatory framework of external departmental and interagency control over the implementation of ETN dictate the need to adjust already established organizational forms of control at the level of relevant control structures, as well as strengthening internal departmental control over the organization of ETN in health care facilities. requirements at the present stage.
Determination of pro-inflammatory and humoral factors in gonarthrosis in women with hypertension and overweight
Authors: Fushtey I. M. , Pidlubna A. M. 
Published: June 2022

Purpose of the study. The purpose of the work. to study the influence of pro-inflammatory cytokines and neurohumoral factors of adipose tissue on the pathogenesis of HA in women with NM and hypertension to further improve the effectiveness of treatment of this group of patients.
Materials and methods. We examined 198 women with GA and GA in combination with GC and NM aged 40 to 70 years (average - 62.6 ± 1.9 years) with a disease duration of an average of 13.4 ± 3.8 years. In order to analyze the impact of comorbid pathology on the underlying disease, all surveyed women were divided into three groups. The first group (G1) included 59 women with symptoms of HA without concomitant pathology, the second (G2) group - 74 women with HA and GC, and the third (G3) 65 women with HA and GC and NM. All patients underwent standard general clinical examination. In order to study the neurohumoral aspects of the disease, enzyme-linked immunosorbent assay of C-reactive protein, tumor necrosis factor, interleukin-1beta, interleukin 6, interleukin 10 and leptin was performed.
Results. It was found that the combination of excess weight with the course of HA leads to a more significant increase in the production of proinflammatory cytokines compared with patients with HA without concomitant pathology (p <0.05). Direct correlations have been established between weight gain and increased levels of proinflammatory cytokines and adipose tissue neurohumoral factors.
Conclusions. The combination of inflammatory and destructive changes in the joints in HA with weight gain and increased blood pressure, creates conditions for the development of severe immunological disorders in patients and requires pharmacological correction.
SERUM LEVEL OF SOME CYTOKINES IN CHILDREN 6-10 YEARS OLD WITH HIGH FREQUENCY OF ACUTE RESPIRATORY INFECTIONS
Authors: Ovcharenko L. S. , Vertegel A. O. , Redko I. I. , Andrienko T. G. , Samohin I. V. , Kryazhev O. V. , Chakmazova O. M. 
Published: June 2022

Objective: improved diagnosis of immune disorders in children aged 6-10 years old, who are often ill od upper respiratory tract infections (URTI), on the basis of these data, further investigation of serum equalities of cytokines, a way of diagnosing with indications in healthy children.
Materials and methods. There were 60 children of 6 to 10 years of age, from which 2 groups were formed: 1). children who are often ill with URTI (n=30); 2). children, who do not get sick often URTI (n=30).
Results. among children of 6-10 years of age, as often ill with URTI, the frequency of registration of high indications in place of interleukin-6 blood serum (by 53.3%, p<0.05), TNF (by 63.3%, p <0.05) and low indications for interleukin-10 (by 33.3%, p<0.05), it was established that there was a high probability (odds ratio) of an increase serum interleukin-6, TNF and a decrease in interleukin-10 as a reference value, it is associated with the presence of an increased incidence of URTI in children.
Conclusion. in children aged 6-10 years, as they often fall ill with URTI, may tend to intensify pro-inflammation of the immune reactions, which still does not ensure a decrease in serious illness on URTI and unsafe side of the development of opportunistic diseases.
PROBLEMS OF ANTIBIOTIC THERAPY IN KOVID-ASSOCIATED HOSPITAL PNEUMONIA
Authors: Savchenko O. A. , Podsevahyna S. L. , Tkachenko A. V. , Myrnyi D. P. , Katsiuba Y. V. , Myrna A. I. 
Published: June 2022

Introduction. The advent of the COVID-19 pandemic has posed challenges to healthcare professionals. The most common manifestation of a new variant of coronavirus disease is pneumonia, and therefore, the doctor is faced with the question of the diagnostic value of certain indicators, as well as - the feasibility of prescribing antibacterial therapy.
The purpose of the study was to assess the importance of clinical, imaging, laboratory methods for diagnosing the condition of patients with covid-associated pneumonia, to determine the nuances of the possible appointment of antibacterial therapy.
Current text. The main problem in the diagnosis of covid-associated pneumonia is the need to differentiate between viral lung disease and the development of secondary bacterial pneumonia. Viral pneumonia can be of varying severity, but does not require antibacterial therapy. At the same time, the accession of bacterial flora on the background of viral lung disease, requires the immediate appointment of antibacterial drugs. It should be noted that the available data to date indicate that the accession of bacterial infection to viral lung disease is confirmed in no more than 8-10% of patients, ie most patients with coronavirus do not require antibiotics [1,2, 3]. At the same time, worldwide, antibiotics associated with pneumonia-associated pneumonia are unreasonably common and, according to some studies, the proportion of patients receiving antibacterial therapy ranges from 27% to 84% [1,4,5].
Conclusions. The problem of managing patients with covid-associated pneumonia is due to the fact that, to date, there is no specific criterion that would clearly distinguish viral lung disease from viral-bacterial. Traditionally, data from clinical, laboratory and instrumental research methods are used for the differential diagnosis of viral and bacterial pneumonia. A common approach to antibiotic treatment in many cases is not only unjustified, but also leads to a massive increase in antibiotic resistance with subsequent complications. In each case, the decision must be made individually, taking into account the full range of diagnostic and clinical aspects.
COMPARISON OF CEMENT AND SCREW FIXATION OF ORTHOPEDIC STRUCTURES
Authors: Kosinov O. S. 
Published: June 2022

In dental prosthetics, every detail is important, because at this stage, not the aesthetic component is put in the first place, but the issue of engraftment of the implant. This will affect the further quality of life and the condition of the patient’s oral cavity. Therefore, in professional circles there are still discussions about which method of fixing the prosthesis is better - cementing or screw fixation, especially since both of them are widely used in dental surgery. This article will discuss the disadvantages and advantages of these methods.
The connection of the implant, abutment and crown can be performed in two ways: fixation on cement - an abutment is fixed on the installed implant with a screw, on which the crown and dental cement are fixed. Screw fixation - the crown is connected to the abutment in advance, in the laboratory, then the finished structure is attached to the implant. For connection, a screw is used, which is inserted into a vertical hole penetrating the crown and abutment. After installing and fixing the crown, a hole remains on the chewing surface (the so-called screw shaft) - it is covered with a composite material.
Of course, the screw design is mainly preferable due to the absence of excess cement. Excess cement is a good basis for biofilm formation. The larger the area of ​​the cement joint, the more bacteria appear. But the doctor is not able to use screw fixation in such situations: inconvenient entry into the implant shaft and there is no straight-line entry of the structure.
Screw fixation has other advantages and disadvantages. The following advantages are distinguished: the crown can be easily removed; construction details fit exactly; there is no danger of cement getting into soft tissues; less work in the clinic and laboratory.
So, when installing a denture, the orthopedist has two options for connecting the main parts of the structure (implant, abutment and crown) - cementing and screw fixation. In the first case, an abutment is fixed on the implant, and a cement-fixed crown is placed on top. During fixation, the screw crown is connected to the abutment in advance, and then the finished structure is attached to the implant itself. The method gets its name from a screw that is inserted into the crown and abutment and leaves a small hole in the crown. This "screw shaft" used to be a serious aesthetic problem, but now it is easily masked by the composite material.
Financiamiento colectivo (crowdfunding) en Uruguay y la región: análisis normativo de una opción de financiamiento
Authors: Montiel Boido Paola Chantal , Suárez Blanco María Macarena 
Published: June 2022

Este artículo tiene como objetivo analizar la evolución que presenta la normativa legal, con sus posteriores reglamentaciones, en Uruguay y países de la región (Argentina, Brasil, Chile, Perú y Colombia); también se analizarán algunas de las principales plataformas de financiamiento colectivo, para visualizar su impacto y evolución. Para ello se propone analizar bibliografía especializada en la temática de los últimos diez años, estudiar y comparar la normativa vigente en el área y finalmente indagar en tres de las plataformas principales que hay activas en estos países, así como también a qué tipo de crowdfunding se dedican. Este análisis se realiza con el objetivo de indagar si es una opción de financiamiento posible y activa en Uruguay y en los países de la región, su impacto en la economía y actividades de los promotores.
En conclusión, es una opción viable en muchos países. En Uruguay su inicio es lento, con poca proyección a futuro, y escasa cantidad de ofertas. Se visualiza la importancia de seguir ahondando en estos medios de financiación, y su impacto en la economía.
Pengaruh Lokasi Usaha, Popularitas Merek, Variasi Produk, Kepercayaan,Gaya Hidup terhadap Minat Beli Ulang KonsumenAliyah Scraf Tulungagung melalui Budaya
Authors: Sari Diah Nurdiana Purnama , Astutiningsih Sri Eka 
Published: June 2022

Penelitian ini bertujuan untuk mengetahui pengaruh langsung dantidak langsung variabel lokasi usaha, popularitas merek, variasi produk, kepercayaan ,gaya hidup terhadap minat beli ulang konsumen Aliyah scraf Tulungagung melalui budaya .Metodologi penelitian yang diunakan adalah pendekatan kuantitatif dengan teknik purposive sampling sebanyak 95 responden. Data yang digperunakan dalam penelitian ini adalahdata primer dan data sekunder yang diuji menggunakan analisis jalur dengan SPSS 25. Hasil penelitian menunjukkan bahwa lokasi usaha, popularitas merek, sertakepercayaan tidak berpengaruh secara langsung dan tidak langsung terhadap minat beli ulang konsumen melalui budaya. Variasi produk dan gaya hidup berpengaruh secara langsung dan tidak langsung terhadap minat beli ulang konsumen melalui budaya. Budaya berpengaruh secara langsung terhadap minat beli ulang konsumen., Adanya penelitian ini diharapkan mampu memberikan pengetahuan terbaru terhadap pengembangan ilmu menejemen untuk meningkatkan minat beli ulang konsumen pada Aliyah scraf secara khusus dan wirausaha lain secara umum.
Sintesis Zeolit X dari Fly Ash Boiler Pabrik Kelapa Sawit sebagai Adsorben Pemurnian Biodiesel
Authors: Oktavianty Herawati 
Published: June 2022

Abstrak
Fly ash boiler pabrik kelapa sawit saat ini menjadi limbah padat yang belum banyak pemanfaatannya. Fly ash tersebut memiliki kandungan silika yang tinggi dimana dapat dimanfaatkan sebagai adsorben. Tujuan dari penelitian ini adalah untuk memproduksi zeolite X dari fly ash boiler pabrik kelapa sawit sebagai adsorben pada pemurnian Biodiesel. Saat ini teknologi pemurnian biodiesel masih didominasi dengan menggunakan pencucian dengan air (wet washing) dimana mengakibatkan limbah cair yang dihasilkan tinggi, sehingga perlu diterapkan teknologi dry washing yang meminimalisir limbah cair serta meningkatkan efisiensi waktu pemurnian. Metode penelitian ini menggunakan metode Response Surface Methodology dengan desain eksperimen berupa Central Composite Design yang terdiri atas 3 faktor yaitu faktor konsentrasi H2SO4, suhu dan waktu kalsinasi adsorben. Pengolahan data dilakukan dengan bantuan software Design Expert 12.0. Tahapan pembuatan adsorben ini meliputi pretreatment fly ash, aktivasi fly ash menjadi adsorben dengan menggunakan larutan asam sulfat (5; 7,5; 10 N), pada suhu 60 °C selama 1 jam, lalu dioven pada suhu 110 °C selama 1 jam untuk selanjutnya dilakukan kalsinasi (T: 500, 600, 700 °C; T: 2, 3, 4 jam). Adsorben yang dihasilkan digunakan pada pemurnian biodiesel dengan metode dry washing, analisis XRD dan BET untuk karakterisasi adsorben dan analisis GC-MS pada biodiesel yang dihasilkan. Dari hasil analisis didapatkan bahwa kadar metil ester biodiesel paling optimal sebesar 67,58% dihasilkan pada sampel dengan variabel konsentrasi H2SO4 7,5 N, suhu kalsinasi 600°C dan waktu kalsinasi 3 jam. Adsorben yang dihasilkan adalah zeolite X yang memiliki luas permukaan 72 m2/g sebelum aktivasi dan 98 m2/g setelah aktivasi.
Analisis Paradiplomasi dalam Kerja Sama Pemerintah Daerah Tulungagung dan United Nations Children’s Fund (UNICEF) dalam Menangani Permasalahan Sosial Anak
Authors: More Aurora Aziza , Kurniawati Dyah Estu 
Published: June 2022

Penelitian ini bertujuan untuk melihat adanya fenomena permasalahan sosial anak di Kabupaten Tulungagung yang berada di Provinsi Jawa Timur yakni merupakan salah satu provinsi terpadat di Indonesia. Di era globalisasi ini, pemerintah terus melakukan upaya untuk mengurangi permasalahan sosial anak tersebut, melalui kerja sama dalam skema paradiplomasi yang dilaksanakan oleh Pemerintah Daerah Kabupaten Tulungagung dengan organisasi internasional yang berfokus dibidang kemanusiaan khususnya kesejahteraan anak dan perempuan yaitu UNICEF. Untuk itu, penulis menggunakan pendekatan paradiplomasi dan peran organisasi internasional dalam memandang upaya dan strategi pemerintah menangani fenomena permasalahan sosial anak. Hasilnya ditemukan bahwa melalui kerja sama tersebut menghadirkan beberapa program seperti pembentukan Unit Layanan Terpadu Perlindungan Sosial anak Integratif (ULT PSAI) dan inovasi pengembangan KLA (Kabupaten Layak Anak). Program tersebut menunjukan progress yang positif, hal ini ditunjukan dengan prestasi yang diraih oleh Pemerintah Kabupaten Tulungagung sebagai Pilot Project Pusat Program Pusat Kesejahteraan Sosial Anak Integratif (PKSAI).