bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Human Conditionally Immortalized Proximal Tubule Epithelial Cell Line as a Novel Model for Studying Senescence and Response to Senolytics
Authors: Yang Yi , Mihajlovic Milos , Valentijn Floris , Nguyen Tri Q. , Goldschmeding Roel , Masereeuw Rosalinde 
Published: March 2022

Accumulating evidence suggests that senescence of kidney tubule epithelial cells leads to fibrosis. These cells secrete senescence-associated secretory phenotype (SASP) factors that are involved in diverse signaling pathways, influencing kidney fibrosis. Here, we investigated whether our previously established conditionally immortalized proximal tubule epithelial cell line overexpressing the organic anion transporter 1 (ciPTEC-OAT1) can be used as a valid in vitro model to study kidney senescence and senolytics response. CiPTEC-OAT1 proliferates rapidly at 33°C and exhibits a “senescence-like” arrest at 37°C, most likely due to suppression of SV40T expression and subsequent reactivation of the p53 and Rb pathways. To understand how permissive (33°C) and non-permissive (37°C) temperatures of the cell culture affect the senescence phenotype, we cultured ciPTEC-OAT1 for up to 12 days and evaluated the apoptosis and SASP markers. Day 0 in both groups is considered as the non-senescence group (control). Further, the potential of navitoclax, dasatinib, quercetin, and the combination of the latter two to clear senescent cells was evaluated. Maturation of ciPTEC-OAT1 at non-permissive temperature affected mRNA and protein levels of senescence markers. A remarkable upregulation in p21 gene expression was found in the non-permissive temperature group, whereas expression of Lamin B1 decreased significantly. SASP factors, including PAI-1A, IL-1β, CTGF, and IL-6 were upregulated, but no significant difference in Bcl-2 and Bcl-xl were found in the non-permissive temperature group. After culturing ciPTEC-OAT1 up to 12 days, cells in the non-permissive temperature group showed an upregulation in the apoptosis-associated proteins Bcl-2, BID, and Bax, and a downregulation in Mcl-1, Bad, Bak, and Bim at various time points. Further, Bcl-xl, Puma, Caspase 3, Caspase 7, and Caspase 9 showed initial upregulations followed by downregulations at later time points. The loss of Lamin B1, upregulation of SA-β-gal expression and increase in its activity, upregulation of p21 levels and downregulation of p53, along with the upregulation of SASP factors, confirmed that maturation at 37°C promotes senescence features. Finally, the senolytics response was evaluated by testing cell viability following exposure to senolytics, to which cells appeared dose-dependently sensitive. Navitoclax was most effective in eliminating senescent cells. In conclusion, culturing ciPTEC-OAT1 at 37°C induces a senescence phenotype characterized by increased expression of cell cycle arrest and anti-apoptosis markers, SASP factors, and responsiveness to senolytics treatment. Therefore, ciPTEC-OAT1 represents a valid model for studying kidney senescence by simply adjusting culture conditions.
Efficacy and Safety of Chinese Patent Medicine for the Prevention and Treatment of Radiotherapy and Chemotherapy-Induced Oral Mucositis: A Systematic Review and Meta-Analysis
Authors: Xie Yufei , Fang Xin , Hua Hong , Zhou Peiru 
Published: March 2022

Background: Radiotherapy and chemotherapy-induced oral mucositis can affect cancer patients’ quality of life, even necessitate cancer therapy and influence prognosis. Chinese patent medicines (CPMs) have been widely used as complementary alternative medicines for the prevention and treatment of oral mucositis, and their efficacy and safety require further evaluation. Therefore, this study was conducted to provide references for clinical practice.Methods: Ten databases were searched electronically and manually to identify randomized controlled trials (RCTs) from their inception to August 2021, concerning the prevention and treatment of radiotherapy and chemotherapy-induced oral mucositis with CPMs. The prevalence, pain level, and the severity of radiotherapy and chemotherapy-induced oral mucositis, as well as the effectiveness rate and adverse effects of CPMs, were set as the outcome criteria. The assessment criteria of the Cochrane Handbook were used to determine study quality and bias, and meta-analysis was conducted using Review Manager 5.4.1 software.Results: A total of 2,312 cases from 27 RCTs were included. Most studies were considered to have a low or unclear risk of bias. More research is available on the use of CPMs in the prevention of radiotherapy and chemotherapy-induced oral mucositis than in its treatment. As for the prevention, it was proved that CPMs could significantly reduce the prevalence of radiotherapy and chemotherapy-induced oral mucositis, especially for the severe types, and decrease pain levels (p &lt; 0.05). For treatment, CPMs could alleviate the symptoms, promote the healing of ulceration in radiotherapy and chemotherapy-induced oral mucositis, and thus improve the efficiency of clinical treatment (p &lt; 0.05). The results of subgroup analyses were mainly consistent with the above results. The adverse effects of CPMs mainly included gastrointestinal reactions and bitter taste, and no serious adverse events were reported.Conclusions: This systematic review and meta-analysis indicated CPMs might be effective for the prevention and treatment of radiotherapy and chemotherapy-induced oral mucositis through reducing the prevalence, decreasing the occurrence of severe types, alleviating the symptoms, and promoting the healing of ulceration. However, due to the limited number of eligible studies and the publication bias, more high-quality, double-blinded, placebo-controlled RCTs are still needed in future research.Systematic Review Registration: [https://inplasy.com/], identifier [INPLASY2021100100].
The Association Between Connectedness and Grit Among Thai In-school Adolescents in Urban Chiang Mai, Thailand
Authors: Tangmunkongvorakul Arunrat , Kelly Matthew , Thongpibul Kulvadee , Musumari Patou Masika , Srithanaviboonchai Kriengkrai , Banwell Cathy 
Published: March 2022

AimTo investigate the associations between Grit, connectedness, and parental involvement in Thai adolescents. Grit, perseverance, and passion for long-term goals are predictors of academic success and health. There is a small but developing knowledge of the predictors of Grit in Asia, especially Thailand. This paper investigates the proposition that connectedness and parental involvement are positively associated with Grit.MethodA total of 2,839 lower secondary (grade 8), higher secondary (grade 11), and vocational (year 12) students from 21 schools in Chiang Mai, Thailand participated in a survey that measured Grit using the Short Grit Scale. Bivariate analysis was conducted using the t-test, ANOVA, or Kruskal–Wallis H test as appropriate. Multiple ordinary least squares linear regression analysis was performed to determine factors associated with Grit.ResultsSatisfactory relationships with teachers (p = 0.01), parental support (p = 0.03), interest in school (p = 0.01), having been asked by parents to do homework (β = −0.69; p = 0.012), and having been told by parents that they had done something bad (β = −1.09; p = 0.02) associated with Grit. These findings can aid in design of tailored interventions to improve Grit in Thai adolescents.
Lifetime Cannabis Use Is Not Associated With Negative Beliefs About Medication in Patients With First Treatment Psychosis
Authors: Gjerde Priyanthi B. , Steen Synne W. , Vedal Trude S. J. , Steen Nils Eiel , Reponen Elina J. , Andreassen Ole A. , Steen Vidar M. , Melle Ingrid 
Published: March 2022

ObjectiveCannabis use is common among patients with psychosis, and along with negative beliefs about medication, it has been found to predict poor adherence to antipsychotic drug treatment. Such lack of adherence to antipsychotic drug treatment increases the risk of poor clinical outcomes and relapse in patients with first treatment for psychosis (FTP). However, to date, it is unclear whether cannabis use may be related to negative perceptions about antipsychotic drug treatment.MethodsA cross-sectional sample of 265 FTP patients with schizophrenia spectrum disorder underwent extensive clinical assessments. Three measures of cannabis use were obtained: lifetime, current and meeting diagnostic criteria for abuse or addiction. For the primary analyses we focused on lifetime cannabis use. The Beliefs about Medication Questionnaire (BMQ) was employed to assess the patients' specific concerns and perceptions of antipsychotic medications, as well as general beliefs about pharmacotherapy. The relationship between lifetime cannabis use and BMQ scores was investigated with general linear model (GLM) analyses, controlling for age and sex.ResultsPatients with lifetime use of cannabis ≥10 times were more likely to be male, younger at the age of onset of psychosis and with higher levels of alcohol use and daily tobacco smoking, as compared to the non-users (p &lt; 0.05). Neither lifetime use of cannabis, current use nor a cannabis abuse diagnosis was associated with negative beliefs about medicines as measured by the BMQ questionnaire.ConclusionUse of cannabis is not linked to negative perceptions about antipsychotic medicines in patients with FTP. Other reasons for poor compliance to antipsychotic drug treatment in cannabis users need to be further investigated.
Impact of Online Courses on University Student Visual Attention During the COVID-19 Pandemic
Authors: Gao Qi , Li Sining 
Published: March 2022

BackgroundUnder the threat of COVID-19, many universities offer online courses to avoid student gatherings, which prevent teachers from collecting responses and optimizing courses. This work collected eye movement data to analyze attention allocation and proposed instruction for improving the courses.MethodsSubjects were recruited to watch three online courses. Meanwhile, their eye movement data were collected through Dikablis Glasses. Mayer’s multimedia cognitive theory was adopted to discriminate the pivotal components of online course, and the Mann–Whitney relevance analysis demonstrated that different representations of courses affected the viewers’ attention differently.ResultsThree subjects watched three different types of political courses. Course 1, which combined text and explanation, attracted the most attention. Course 2 was shown to be less attractive than course 1 and better than course 3, but the subjects were distracted by the animations in course 2. Course 3, which did not use any technique to present learning content, attracts the least attention from the subjects. A correlation analysis shows that course 1 and course 3 have similar results compared with course 2.ConclusionOnline courses have become a norm during the COVID-19 pandemic. Improving the quality of online courses can effectively reduce the impact of the epidemic on teaching. These experiment results suggest that text + commentary in the design of online courses can effectively attract the attention of the listeners and achieve better learning results. Attention gradually rises in the early stage and then falls after reaching a peak. At this time, the proper introduction of animation can effectively reverse the attention curve, while individual text or commentary results in quickly losing the listener’s attention.
Comparison of Clinical Data Between Patients With Complications and Without Complications After Spinal Tuberculosis Surgery: A Propensity Score Matching Analysis
Authors: Chen Liyi , Liu Chong , Ye Zhen , Chen Wuhua , Sun Xuhua , Chen Jiarui , Li Hao , Liang Tuo , Huang Shengsheng , Jiang Jie , Chen Tianyou , Guo Hao , Yao Yuanlin , Liao Shian , Yu Chaojie , Wu Shaofeng , Fan Binguang , Zhan Xinli 
Published: March 2022

PurposeThis study used a propensity score matching (PSM) analysis to explore the risk factors of post-operative complications and compared the differences in clinical data between them following spinal tuberculosis surgery.MethodsThe clinical data of patients with spinal tuberculosis were collected in our hospital from June 2012 to June 2021, including general information, laboratory results, surgical information, and hospitalization costs. The data were divided into two groups: complication and without complication groups. The baseline data of the two groups were obtained using the PSM analysis. Univariate and multivariate logistic analyses were used to analyze the differences between the two groups.ResultsA total of 292 patients were included in the PSM analysis: 146 patients with complications and 146 patients without complications. The operation time, incision length, hospital stay, and albumin quantity in the complications group were 162 ± 74.1, 11.2 ± 4.76, 14.7 ± 9.34, and 1.71 ± 2.82, respectively, and those in the without complication group were 138 ± 60.5, 10.2 ± 3.56, 11.7 ± 7.44, and 0.740 ± 2.44, respectively. The laboratory costs, examination costs, guardianship costs, oxygen costs, and total costs in the complications group were higher than those in the without complication group. A significant difference was observed in the albumin quantity by logistic regression analysis (P &lt; 0.05).ConclusionSeveral costs in the complication group were higher than in the without complication group. The albumin quantity may be an independent factor to predict post-operative complications of spinal tuberculosis by logistic regression analysis.
A Solution of a Boundary Value Problem with Mixed Conditions for a Four-Dimensional Degenerate Elliptic Equation
Authors: Baishemirov Zharasbek , Berdyshev Abdumauvlen , Ryskan Ainur 
Published: March 2022

The solvability issues of counterpart Holmgren’s boundary value problem with mixed conditions for a degenerate four-dimensional second-order Gellerstedt equation Hu≡ymzktluxx+xnzktluyy+xnymtluzz+xnymzkutt=0, m,n,k,l≡const>0, are studied in the finite domain R4+, where the values of normal derivatives are set on the piecewise smooth part of the boundary and the values of the desired function are set on the remaining part of the boundary. The main results of the work are the proof of the uniqueness of the considered problem solution by using an energy integral’s method and the construction of the solution of counterpart Holmgren’s boundary value problem in explicit form by means of Green’s function method, containing the hypergeometric Lauricella’s function FA4. Using the corresponding fundamental solution for the considered generalized Gellerstedt equation of elliptic type, we construct Green’s function. In addition, formulas of differentiation, some adjacent relations, decomposition formulas, and various properties of Lauricella’s hypergeometric functions were used to establish the main results for the aforementioned problem.
INTEGRATED APPROACH AS A MEANS OF FORMATION OF LEGAL COMPETENCE OF FUTURE MANAGEMENT MANAGERS OF EDUCATIONAL ESTABLISHMENTS IN THE CONDITIONS OF A MAGISTRACY
Authors: BARDINOVA A. 
Published: March 2022

The article, based on a theoretical study, scientifically substantiates the need for the formation of legal competence of future management managers of educational establishments in the conditions of a magistracy. The semantic essence and significance of the integrated approach as a powerful means of forming the studied formation are revealed.
The authors argue that the organization of classes on the basis of an integrative approach is pedagogically appropriate, because they accelerate the process of adaptation of students, future managers of educational institutions to practical situations, develop skills in using theoretical knowledge of various disciplines in conjunction with acquired practical legal skills. , contribute to the structuring of the integrity of knowledge on the research problem. Therefore, they really reflect the integration processes in the formation of legal competence of future managers in the management of educational institutions in the master's degree.
Further research of the problem is seen in clarifying the purpose, content and means of formation of legal competence in future managers of the management of educational institutions in the master's degree through the integration of only professional disciplines.
Perennial Allergic Rhinitis: Prospects for Treatment with Azelastine Nasal Spray
Authors: Сакович А.Р. 
Published: March 2022

В данной статье рассмотрены диагностика и лечение круглогодичного аллергического ринита (КАР). Пациентам 1-й группы назначалась монотерапия (назальный спрей азеластина (аллергодил)), пациентам 2-й группы назначалась комбинированная терапия (назальный спрей азеластина в сочетании с интраназальным глюкокортикостероидом (ИГКС) (флутиказон)). Проведенное исследование подтвердило более высокую эффективность сочетанного применения азеластина (аллергодила) и ИГКС у пациентов с КАР по сравнению с монотерапией азеластином (аллергодилом).
This article discusses the diagnosis and treatment of perennial allergic rhinitis (PAR). Patients in group 1 were prescribed monotherapy (azelastine nasal spray (Allergodil)), patients in group 2 were prescribed combined therapy (azelastine nasal spray in combination with intranasal corticosteroids (fluticasone)). The study confirmed the higher efficacy of combined use of azelastine (Allergodil) and intranasal corticosteroid in patients with PAR compared with monotherapy with azelastine (Allergodil).
Pedagogika ignacjańska w świecie VUCA
Authors: Dybowska Ewa 
Published: March 2022

CEL NAUKOWY: Celem artykułu jest ukazanie charakterystycznego dynamizmu pedagogiki ignacjańskiej, która może pomóc współczesnemu człowiekowi odnajdywać orientację życiową ukierunkowaną na najważniejszy cel oraz poczucie sensu. Celem jest także rozszerzenie zastosowania modelu pedagogiki ignacjańskiej poza rzeczywistością edukacyjną.
PROBLEM I METODY BADAWCZE: Rozwinięty w artykule problem badawczy brzmi: Co ma do zaproponowania pedagogika ignacjańska człowiekowi żyjącemu w rzeczywistości określanej jako świat VUCA? Poszukując odpowiedzi, zastosowano analizę literatury.
PROCES WYWODU: W artykule krótko scharakteryzowano rzeczywistość VUCA, odnosząc ją do codziennego życia. Wskazano potrzebę posiadania wskazówek dla efektywnego poruszania się po doświadczaniu życia. Tych wskazówek poszukiwano w dynamice modelu pedagogiki ignacjańskiej. Wskazano, jak można dostosować ów model do funkcjonowania w rzeczywistości VUCA.
WYNIKI ANALIZY NAUKOWEJ: Pozostając w logice ignacjańskiej zasady dostosowania do osób, miejsca i czasu, wykazano, jak dynamizm pedagogiki ignacjańskiej może współczesnemu człowiekowi pomóc znajdować poczucie własnej pełnomocności.
WNIOSKI, INNOWACJE, REKOMENDACJE: Warto, aby współczesny człowiek nie ustawał w poszukiwaniu celu ostatecznego i poczucia sensu w doświadczaniu życia. Pedagogika ignacjańska może stanowić odpowiedź czy antidotum na świat generujący chaos wynikający z przenikania się w nim zmienności, niepewności, złożoności i niejednoznaczności. Rekomendacja taka jest możliwa dzięki ignacjańskiej zasadzie dostosowania do osób, miejsca i czasu.
Koncepcja teatru jezuickiego i jego znaczenie w procesie socjalizacji młodzieży szkolnej
Authors: Charchuła SJ Jarosław 
Published: March 2022

CEL NAUKOWY: Celem niniejszego opracowania jest omówienie funkcji, jaką pełni teatr jezuicki w procesie socjalizacji młodzieży szkolnej.
PROBLEM I METODY BADAWCZE: Główny problem badawczy dotyczy odpowiedzi na pytanie: jakie warunki muszą być spełnione, aby teatr jezuicki mógł być skutecznym narzędziem procesu socjalizacji młodzieży szkolnej. W artykule zastosowano metodę analityczno-syntetyczną literatury przedmiotu.
PROCES WYWODU: Artykuł jest skonstruowany w następujący sposób: Na wstępie ukazano najważniejsze cechy koncepcji teatru jezuickiego. Następnie zarysowano ramy teoretyczne, w jakich analizowane są procesy socjalizacji w świetle nauk społecznych, co wreszcie pozwoliło na określenie najważniejszych wyzwań stających przed szkolnictwem jezuickim w kontekście wykorzystania funkcji socjalizacyjnej działalności teatralnej.
WYNIKI ANALIZY NAUKOWEJ: Z przedstawionych rozważań wynika, że jest możliwe włączenie działalności teatralnej jako skutecznego narzędzia socjalizacji młodzieży w szkołach jezuickich. Co więcej, wydaje się, że jezuicki model edukacji stanowi adekwatną odpowiedź na zmiany spowodowane procesami globalizacji.
WNIOSKI, INNOWACJE, REKOMENDACJE: Wnioski, jakie wynikają z przedstawionych analiz, wskazują przede wszystkim na komplementarność procesu edukacji i socjalizacji realizowanej poprzez wykorzystanie narzędzia, jakim jest teatr jezuicki. Tożsamość jezuickiego szkolnictwa wynika w dużym stopniu z przyjęcia całościowej wizji osoby i integralnego modelu formacji. To połącznie edukacji z działalnością teatralną wydaje się trafną receptą na aktualne wyzwania procesu socjalizacji młodzieży szkolnej.
Applying the language acquisition model to the solution small language processing tasks
Authors: Kachkou Dz. I. 
Published: March 2022

The problem of building a computer model of a small language was under solution. The relevance of this task is due to the following considerations: the need to eliminate the information inequality between speakers of different languages; the need for new tools for the study of poorly understood languages, as well as innovative approaches to language modeling in the low-resource context; the problem of supporting and developing small languages.There are three main objectives in solving the problem of small natural language processing at the stage of describing the problem situation: to justify the problem of modeling language in the context of resource scarcity as a special task in the field of natural languages processing, to review the literature on the relevant topic, to develop the concept of language acquisition model with a relatively small number of available resources. Computer modeling techniques using neural networks, semi-supervised learning and reinforcement learning were involved.The paper provides a review of the literature on modeling the learning of vocabulary, morphology, and grammar of a child's native language. Based on the current understanding of the language acquisition and existing computer models of this process, the architecture of the system of small language processing, which is taught through modeling of ontogenesis, is proposed. The main components of the system and the principles of their interaction are highlighted. The system is based on a module built on the basis of modern dialogical language models and taught in some rich-resources language (e.g., English). During training, an intermediate layer is used which represents statements in some abstract form, for example, in the symbols of formal semantics. The relationship between the formal recording of utterances and their translation into the target low-resource language is learned by modeling the child's acquisition of vocabulary and grammar of the language. One of components stands for the non-linguistic context in which language learning takes place.This article explores the problem of modeling small languages. A detailed substantiation of the relevance of modeling small languages is given: the social significance of the problem is noted, the benefits for linguistics, ethnography, ethnology and cultural anthropology are shown. The ineffectiveness of approaches applied to large languages in conditions of a lack of resources is noted. A model of language learning by means of ontogenesis simulation is proposed, which is based both on the results obtained in the field of computer modeling and on the data of psycholinguistics.