bluuebunny/crossref_metadata_2025_split huggingface dataset:
SIRT1 in the cardiomyocyte counteracts doxorubicin-induced cardiotoxicity via regulating histone H2AX
Authors: Kuno Atsushi , Hosoda Ryusuke , Tsukamoto Miki , Sato Tatsuya , Sakuragi Hiromi , Ajima Nami , Saga Yukika , Tada Kouhei , Taniguchi Yoshiki , Iwahara Naotoshi , Horio Yoshiyuki 
Published: March 2022

Abstract

Aims
Cardiotoxicity by doxorubicin predicts worse prognosis of patients. Accumulation of damaged DNA has been implicated in doxorubicin-induced cardiotoxicity. SIRT1, an NAD+-dependent histone/protein deacetylase, protects cells by deacetylating target proteins. We investigated whether SIRT1 counteracts doxorubicin-induced cardiotoxicity by mediating Ser139 phosphorylation of histone H2AX, a critical signal of the DNA damage response.

Methods and results
Doxorubicin (5 mg/kg per week, x4) was administered to mice with intact SIRT1 (Sirt1f/f) and mice that lack SIRT1 activity in cardiomyocytes (Sirt1f/f;MHCcre/+). Reductions in left ventricular fractional shortening and ejection fraction by doxorubicin treatment were more severe in Sirt1f/f;MHCcre/+ than in Sirt1f/f. Myocardial expression level of type-B natriuretic peptide was 2.5-fold higher in Sirt1f/f;MHCcre/+ than in Sirt1f/f after doxorubicin treatment. Sirt1f/f;MHCcre/+ showed larger fibrotic areas and higher nitrotyrosine levels in the heart after doxorubicin treatment. Although doxorubicin-induced DNA damage evaluated by TUNEL staining was enhanced in Sirt1f/f;MHCcre/+, the myocardium from Sirt1f/f;MHCcre/+ showed blunted Ser139 phosphorylation of H2AX by doxorubicin treatment. In H9c2 cardiomyocytes, SIRT1 knockdown attenuated Ser139 phosphorylation of H2AX, increased DNA damage, and enhanced caspase-3 activation under doxorubicin treatment. Immunostaining revealed that acetylation level of H2AX at Lys5 was higher in hearts from Sirt1f/f;MHCcre/+. In H9c2 cells, acetyl-Lys5-H2AX level was increased by SIRT1 knockdown and reduced by SIRT1 overexpression. Ser139 phosphorylation in response to doxorubicin treatment was blunted in a mutant H2AX with substitution of Lys5 to Gln (K5Q) that mimics acetylated lysine compared with that in wild-type H2AX. Expression of K5Q-H2AX as well as S139A-H2AX, which cannot be phosphorylated at Ser139, augmented doxorubicin-induced caspase-3 activation. Treatment of mice with resveratrol, a SIRT1 activator, attenuated doxorubicin-induced cardiac dysfunction, which was associated with a reduction in acetyl-Lys5-H2AX level and a preserved phospho-Ser139-H2AX level.

Conclusion
These findings suggest that SIRT1 counteracts doxorubicin-induced cardiotoxicity by mediating H2AX phosphorylation through its deacetylation in cardiomyocytes.
Population Source of Third-Generation Oriental Armyworm in Jilin, China, Determined by Entomology Radar, Trajectory Analysis, and Mitochondrial COI Sequences
Authors: Sun Wei , Hu Gao , Su Qianfu , Wang Yangzhou , Yang Wei , Zhou Jiachun , Gao Yuebo 
Published: March 2022

AbstractThe armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae), is an important polyphagous pest with a strong migratory ability. Recently, third-generation larvae have become an increasingly serious pest threat in Jilin Province of northeast China. To investigate the population source of this species, scanning entomological radar observations and insect mitochondrial cytochrome oxidase I (COI) genes were used in this study. Five main results were found: (1) The peak period in captured second-generation moths was from mid to late July. The temperature and wind speeds were optimum for the moths to have migrated. Strong southwesterly winds occurred during the peak migration period. (2) Radar observations indicated that most of the moths’ migration took place at a height of 600 m, often in a dense layer which formed at heights of 350–800 m. (3) Analyses of adult ovarian development and larval haplotypes showed third-generation larvae were progeny of both locally produced progeny and immigrant moths. (4) Based on our back-tracking and haplotype analyses, immigration led to an outbreak originated in the same source area to the southwest. (5) Emigration of second-generation moths was confirmed by both radar observation and mtDNA analysis. Forward trajectories indicated that the moths were capable of immigrating far from their overwintering range. These results are useful for improving the forecasting systems of this insect pest species.
Multi-chamber speckle tracking imaging and diagnostic value of left atrial strain in cardiac amyloidosis
Authors: Aimo Alberto , Fabiani Iacopo , Giannoni Alberto , Mandoli Giulia Elena , Pastore Maria Concetta , Vergaro Giuseppe , Spini Valentina , Chubuchny Vladyslav , Pasanisi Emilio Maria , Petersen Christina , Poggianti Elisa , Taddei Claudia , Castiglione Vincenzo , Latrofa Sara , Panichella Giorgia , Sciaccaluga Carlotta , Georgiopoulos Georgios , Passino Claudio , Cameli Matteo , Emdin Michele 
Published: March 2022

Abstract

Aims
Cardiac amyloidosis (CA) affects the four heart chambers, which can all be evaluated through speckle-tracking echocardiography (STE).

Methods and results
We evaluated 423 consecutive patients screened for CA over 5 years at two referral centres. CA was diagnosed in 261 patients (62%) with either amyloid transthyretin (ATTR; n = 144, 34%) or amyloid light-chain (AL; n = 117, 28%) CA. Strain parameters of all chambers were altered in CA patients, particularly those with ATTR-CA. Nonetheless, only peak left atrial longitudinal strain (LA-PALS) displayed an independent association with the diagnosis of CA or ATTR-CA beyond standard echocardiographic variables and cardiac biomarkers (Model 1), or with the diagnosis of ATTR-CA beyond the validated IWT score in patients with unexplained left ventricular (LV) hypertrophy. Patients with the most severe impairment of LA strain were those most likely to have CA or ATTR-CA. Specifically, LA-PALS and/or LA-peak atrial contraction strain (PACS) in the first quartile (i.e. LA-PALS &lt;6.65% and/or LA-PACS &lt;3.62%) had a 3.60-fold higher risk of CA, and a 3.68-fold higher risk of ATTR-CA beyond Model 1. Among patients with unexplained LV hypertrophy, those with LA-PALS or LA-PACS in the first quartile had an 8.76-fold higher risk for CA beyond Model 1, and a 2.04-fold higher risk of ATTR-CA beyond the IWT score.

Conclusions
Among STE measures of the four chambers, PALS and PACS are the most informative ones to diagnose CA and ATTR-CA. Patients screened for CA and having LA-PALS and/or LA-PACS in the first quartile have a high likelihood of CA and ATTR-CA.
The genome organization of Neurospora crassa at high resolution uncovers principles of fungal chromosome topology
Authors: Rodriguez Sara , Ward Ashley , Reckard Andrew T , Shtanko Yulia , Hull-Crew Clayton , Klocko Andrew D 
Published: March 2022

Abstract
The eukaryotic genome must be precisely organized for its proper function, as genome topology impacts transcriptional regulation, cell division, replication, and repair, among other essential processes. Disruptions to human genome topology can lead to diseases, including cancer. The advent of chromosome conformation capture with high-throughput sequencing (Hi-C) to assess genome organization has revolutionized the study of nuclear genome topology; Hi-C has elucidated numerous genomic structures, including chromosomal territories, active/silent chromatin compartments, Topologically Associated Domains, and chromatin loops. While low-resolution heatmaps can provide important insights into chromosomal level contacts, high-resolution Hi-C datasets are required to reveal folding principles of individual genes. Of particular interest are high-resolution chromosome conformation datasets of organisms modeling the human genome. Here, we report the genome topology of the fungal model organism Neurospora crassa at a high resolution. Our composite Hi-C dataset, which merges 2 independent datasets generated with restriction enzymes that monitor euchromatin (DpnII) and heterochromatin (MseI), along with our DpnII/MseI double digest dataset, provide exquisite detail for both the conformation of entire chromosomes and the folding of chromatin at the resolution of individual genes. Within constitutive heterochromatin, we observe strong yet stochastic internal contacts, while euchromatin enriched with either activating or repressive histone post-translational modifications associates with constitutive heterochromatic regions, suggesting intercompartment contacts form to regulate transcription. Consistent with this, a strain with compromised heterochromatin experiences numerous changes in gene expression. Our high-resolution Neurospora Hi-C datasets are outstanding resources to the fungal community and provide valuable insights into higher organism genome topology.
The wing imaginal disc
Authors: Tripathi Bipin Kumar , Irvine Kenneth D 
Published: March 2022

AbstractThe Drosophila wing imaginal disc is a tissue of undifferentiated cells that are precursors of the wing and most of the notum of the adult fly. The wing disc first forms during embryogenesis from a cluster of ∼30 cells located in the second thoracic segment, which invaginate to form a sac-like structure. They undergo extensive proliferation during larval stages to form a mature larval wing disc of ∼35,000 cells. During this time, distinct cell fates are assigned to different regions, and the wing disc develops a complex morphology. Finally, during pupal stages the wing disc undergoes morphogenetic processes and then differentiates to form the adult wing and notum. While the bulk of the wing disc comprises epithelial cells, it also includes neurons and glia, and is associated with tracheal cells and muscle precursor cells. The relative simplicity and accessibility of the wing disc, combined with the wealth of genetic tools available in Drosophila, have combined to make it a premier system for identifying genes and deciphering systems that play crucial roles in animal development. Studies in wing imaginal discs have made key contributions to many areas of biology, including tissue patterning, signal transduction, growth control, regeneration, planar cell polarity, morphogenesis, and tissue mechanics.
Fiscal resiliency in a deeply uncertain world: The role of semiautonomous discretion
Authors: Orszag Peter R , Rubin Robert E , Stiglitz Joseph E 
Published: March 2022

AbstractThis Policy Brief argues that embracing the deep uncertainty about interest rates and other key parameters determining a country’s fiscal position requires a new approach in fiscal policy. It proposes retaining fiscal discretion exercised after making the budget adjust more automatically and rapidly in areas where there is broad consensus that doing so is consistent with achieving broader societal goals. For such a semiautonomous discretionary fiscal architecture five elements are emphasized: stronger automatic stabilizers, a new infrastructure program, extension of debt maturities, indexation of long-term fiscal programs to their underlying drivers, and more emphasis on residual fiscal discretion. The goal is to provide a framework better adapted than our current budgetary structures to deep uncertainty, thus freeing up a discretionary fiscal policy to focus more on adjusting to the unanticipated.
State Responsibility and Compliance with Provisional Measures under ICSID
Authors: McRae Donald 
Published: March 2022

Abstract
Article 47 of the ICSID Convention, grants tribunals authority to “recommend” provisional measures. However, ICSID tribunals have concluded that the word ‘recommend’ is imperative and that ‘recommendations’ for provisional measures are binding. Yet no plausible reason has ever been advanced to justify treating the word ‘recommend’ as the equivalent of ‘order’. An application of Articles 31-33 of the Vienna Convention on the Law of Treaties does not support such a result, and analogies with the practice of the International Court of Justice and the US-Iran Claims Tribunal ignore critical wording differences. The Articles on State Responsibility provide important guidance on why States do in fact have an obligation to comply with provisional measures granted by an ICSID tribunal even though they are only in the form of a recommendation. An internationally wrongful act is a ‘violation by a State of any obligation’. An obligation on participants in international arbitration not to aggravate the dispute is an obligation recognized by international courts and tribunals and equally applicable to investment arbitration. Thus, if failure to comply with provisional measures recommended by an ICSID tribunal amounts to an aggravation of the dispute, then the State concerned has committed an internationally wrongful act for which it is responsible.
Cryotherapy Induced Burns: A Case Series of Three Patients
Authors: Ellis Ryan C , Kohler Casey L , Dvorak Justin E 
Published: March 2022

Abstract
Whole-body cryotherapy involves the use of a specially designed liquid nitrogen cryochamber to expose the body to cold, dry air (below −100°C) for short periods of time and has been gaining popularity in the field of sports and exercise science as an aid in recovery following exercise or injury. While proponents of cryotherapy claim that it can reduce inflammation and expedite muscle recovery, there has yet to be published literature that supports these claims. While there is no evidence that cryotherapy provides any benefits, there is a risk for injury with the use of these devices owing to the extreme cold temperatures that are used. We present the three cases of patients who suffered frostbite injuries secondary to single cryotherapy sessions. The first case involved a 26-year-old male who suffered a full-thickness frostbite injury that eventually required excision and grafting, while cases 2 and 3 involve a 17-year-old male and a 28-year-old male who both suffered partial-thickness injuries.
Long-Term Survival and Causes of Death After Diagnoses of Common Cancers in 3 Cohorts of US Health Professionals
Authors: Cheng En , Lee Dong Hoon , Tamimi Rulla M , Hankinson Susan E , Willett Walter C , Giovannucci Edward L , Eliassen A Heather , Stampfer Meir J , Mucci Lorelei A , Fuchs Charles S , Spiegelman Donna 
Published: March 2022

Abstract

Background
Few studies investigated long-term overall survival and causes of death among men and women diagnosed with most commonly occurring cancers.

Methods
We estimated long-term (≥30-year) overall and cause-specific cumulative mortality for men diagnosed with prostate (n = 6873), lung and bronchus (n = 1290), colon and rectum (n = 1418), bladder (n = 1321), and melanoma (n = 2654) cancer in the Health Professionals Follow-up Study between 1986 and 2012 and women with breast (n = 18 280), lung and bronchus (n = 3963), colon and rectum (n = 3461), uterine corpus (n = 1641), and thyroid (n = 1103) cancer in the Nurses’ Health Study between 1976 and 2012 and Nurses’ Health Study II between 1989 and 2013.

Results
We reported overall and cause-specific cumulative mortality of 30 years among men and 35 years among women. Among male cancer survivors, the 30-year cumulative cancer-specific mortality was 15.4% (95% confidence interval [CI] = 14.4% to 16.4%) for prostate, 83.5% (95% CI = 81.2% to 85.5%) for lung and bronchus, 37.0% (95% CI = 34.4% to 39.5%) for colon and rectum, 22.5% (95% CI = 20.0% to 25.0%) for urinary bladder, and 8.0% (95% CI = 6.9% to 9.1%) for melanoma. Among female cancer survivors, the 35-year cumulative cancer-specific mortality rate was 20.6% (95% CI = 19.7% to 21.6%) for breast, 83.5% (95% CI = 81.6% to 85.2%) for lung and bronchus, 39.6% (95% CI = 37.5% to 41.6%) for colon and rectum, 16.6% (95% CI = 14.7% to 18.6%) for uterine corpus, and 3.2% (95% CI = 2.1% to 4.3%) for thyroid. Except for lung cancer, most patients with common cancer were more likely to die from causes other than primary cancers. We observed 2 basic trends for cumulative cancer-specific mortality. The first is a sustained but nevertheless excess risk: Prostate or breast cancer-specific cumulative mortality continued to increase after diagnosis from 5 to 30 years or longer. The second is greatly diminished risk of index cancer-specific mortality following diagnosis 10 years or longer previously. For example, colorectal cancer–specific mortality increased by less than 4 percentage points between 10 and 30 or 35 years after diagnosis, and this finding also applied to lung, bladder, melanoma, uterine corpus, and thyroid cancer.

Conclusions
Except for lung cancer, patients diagnosed with common cancers were more likely to die from causes other than primary cancers. Patients with lung, colorectal, bladder, melanoma, uterine corpus, or thyroid cancer surviving longer than 10 years after diagnosis are unlikely to die from that disease.
Evidence of functional and structural changes in the microbial community beneath a succulent invasive plant in coastal dunes
Authors: Souza-Alonso Pablo , Lechuga-Lago Yaiza , Guisande-Collazo Alejandra , González Luís 
Published: March 2022

Abstract
Coastal dunes represent priority habitats for conservation due to the provision of valuable ecosystem services such as land protection, water supply or biodiversity conservation. Soil microbial communities are of crucial importance to maintain plant diversity due to harsh environmental conditions, water limitation and nutrient scarcity. Invasive alien plants represent a major threat to ecosystem conservation. Here, we explored different impacts of Carpobrotus edulis, a succulent plant invading coastal areas worldwide, on the function and structure of bacterial communities. Sand represents a challenging substrate due to low organic matter content and limited microbial activity. We optimized bacterial extraction for functional evaluation before assessing ecosystem impacts produced by C. edulis. We compared 12 extracting procedures combining different soil storage, sample amount and extracting solutions on the functional activity of sand communities through the community-level physiological profile. We further explored the function (using Biolog Ecoplates) and structure [using polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE)] of bacterial communities from dunes invaded by C. edulis. Saline solution consistently increased bacterial cells detected by cytometry (P ≤ 0.001). Principal component analysis suggested a limited temporal framework (0–24 h) in which community function can be explored without significant alterations in C substrate consumption. Changes under C. edulis invasion exhibited a different pattern of C substrate utilization comparing native and non-native zones (interspecific), but also between native zones (intraspecific), suggesting that functional impacts are site-dependent. Complementary, results obtained from PCR-DGGE indicated that the bacterial community structure of native dunes significantly differed from dunes invaded by C. edulis.
Development of PHITS graphical user interface for simulation of positron emitting radioisotopes production in common biological materials during proton therapy
Authors: Shahmohammadi Beni Mehrdad , Yu Kwan Ngok , Islam M Rafiqul , Watabe Hiroshi 
Published: March 2022

Abstract
The Monte Carlo (MC) method is a powerful tool for modeling nuclear radiation interaction with matter. A variety of MC software packages has been developed, especially for applications in radiation therapy. Most widely used MC packages require users to write their own input scripts for their systems, which can be a time consuming and error prone process and requires extensive user experience. In the present work, we have developed a graphical user interface (GUI) bundled with a custom-made 3D OpenGL visualizer for PHITS MC package. The current version focuses on modeling proton induced positron emitting radioisotopes, which in turn can be used for verification of proton ranges in proton therapy. The developed GUI program does not require extensive user experience. The present open-source program is distributed under GPLv3 license that allows users to freely download, modify, recompile and redistribute the program.
An 8 per cent determination of the Hubble constant from localized fast radio bursts
Authors: Wu Qin , Zhang Guo-Qiang , Wang Fa-Yin 
Published: March 2022

ABSTRACT
The cosmological-constant (Λ) cold dark matter (CDM) model is challenged by the Hubble tension, a remarkable difference of Hubble constant H0 between measurements from local probes and the prediction from Planck cosmic microwave background observations under ΛCDM model. So one urgently needs new distance indicators to test the Hubble tension. Fast radio bursts (FRBs) are millisecond-duration pulses occurring at cosmological distances, which are attractive cosmological probes. Here, we report a measurement of ${H_0} = 68.81^{+4.99}_{-4.33} {\rm \ km \ s^{-1} \ Mpc^{-1}}$ using eighteen localized FRBs, with an uncertainty of 8 per cent at 68.3 per cent confidence. Using a simulation of 100 localized FRBs, we find that error of H0 can be reduced to 2.6 per cent at 1σ uncertainty. Thanks to the high event rate of FRBs and localization capability of radio telescopes (i.e. Australian Square Kilometre Array Pathfinder and Very Large Array), future observations of a reasonably sized sample will provide a new way of measuring H0 with a high precision to test the Hubble tension.
A hot and luminous source at the site of the fast transient AT2018cow at 2–3 yr after its explosion
Authors: Sun Ning-Chen , Maund Justyn R , Crowther Paul A , Liu Liang-Duan 
Published: March 2022

ABSTRACT
We report the discovery of a luminous late-time source at the position of the fast blue optical transient AT2018cow on images taken by the Hubble Space Telescope at 714 d and 1136 d after its explosion. This source is detected at both UV and optical wavelengths and has prominent Hα emission. It has a very stable brightness between the two epochs and a very blue spectral energy distribution consistent with fλ $\propto \, \lambda ^{-4.1 \pm 0.1}$, i.e. the Rayleigh–Jeans tail of a hot blackbody with a very high temperature of log(T/K) &gt; 4.7 and luminosity of log(L/L⊙) &gt; 7.0. This late-time source is unlikely to be an unrelated object in chance alignment, or due to a light echo of AT2018cow. Other possible scenarios also have some difficulties in explaining this late-time source, including companion star(s), star cluster, the survived progenitor star, interaction with circumstellar medium, magnetar, or delayed accretion in a tidal disruption event. Long-term and multi-wavelength monitoring will help to resolve its nature and finally reveal the origin of the ‘Cow’.