bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overlying rock movement and mining pressure in a fully mechanized caving face with a large dip angle
Authors: Chen Ying , Wang Zhiwen , Hui Qianjia , Zhu Zhijie , Sun Dequan , Chen Yang , Zhang Xiufeng , Wang Zhaoyi , Wang Jian , Zhao Jian 
Published: August 2022

The overlying rock movement and mining pressure in the fully mechanized caving face with a large dip angle were systematically investigated according to theoretical analysis, similar material simulation, numerical calculation, and field monitoring. The following conclusions were obtained: 1) the theoretical analysis showed that the roof movement characteristics at different positions of the working face are quite different. The mining pressure in the upper section of the working face is primarily controlled by the structural instability of the main roof. The stable structure of the main roof is easily formed in the middle and lower sections of the working face, and the mining pressure is mostly controlled by the collapse of the immediate roof. 2) The results of similar material simulations indicated that the height of the fracture zone in different areas of the working face is different due to the influence of a large dip angle. The height of the fracture zone formed in the upper section of the working face is significantly larger than that formed in the lower section of the working face. 3) The numerical calculation suggested that the residual coal pillar of the overlying coal seam has a certain influence on the mining pressure of the 9-301 working face, making the advanced abutment pressure in the range of 80 m close to the main gate under the coal pillar more obvious. 4) The field pressure monitoring results demonstrated that the influence range of the advanced abutment pressure close to the upper part of the working face is greater than that close to the lower part of the working face, and the peak point is closer to the mining rib. Affected by the overlying residual coal pillar, the hydraulic support resistance and the peak value of advanced abutment pressure in the lower part of the working face are greater than those in the upper part. Both the initial and periodic pressure intervals of the upper section of the working face are smaller than those of the lower section. The results of this research will provide a scientific basis for the reasonable determination of the control measures for the mining pressure.
Effects of liraglutide or lifestyle interventions combined with other antidiabetic drugs on abdominal fat distribution in people with obesity and type 2 diabetes mellitus evaluated by the energy spectrum ct: A prospective randomized controlled study
Authors: Yu Dongni , Zou Mingzhu , Pan Qi , Song Yan , Li Miao , Zhang Xianbo , Zhou Yan , Wang Xiaoxia , Guo Lixin 
Published: August 2022

ObjectiveTo study the effects of liraglutide or lifestyle interventions combined with other antidiabetic drugs on glucose metabolism and abdominal fat distribution in patients with obesity and type 2 diabetes mellitus (T2DM).MethodsFrom April 30, 2020, to April 30, 2022, a prospective randomized controlled study was carried out at the Endocrinology Department of Beijing Hospital, the National Center of Gerontology. According to the in- and exclusion criteria and by the random table method, revisited T2DM patients were selected as the research subjects and were allocated into a Study group (taking liraglutide) and a Control group (underwent lifestyle interventions). All patients received continuous 12-weeks interventions to the endpoint, and the changes of value [Δ=(endpoint)-(baseline)] of physical measurements, blood tests, the energy spectrum CT examination results, and body composition analysis results were analyzed and compared.ResultsA total of 85 people completed this study, and among them, 47 were in the Study group and 38 were in the Control group. Compared with the Control group, the changes of hemoglobin A1c (HbA1c) level (-0.78 ± 1.03% vs. -1.57 ± 2.00%, P=0.025), visceral fat area (0.91 ± 16.59 cm2 vs. -7.1 ± 10.17 cm2, P=0.011), and subcutaneous fat area of abdomen [0 (-18.75, 15.5) cm2 vs. -16.5 (-41.75, -2.25) cm2, P=0.014] were all greater in the Study group. The adverse events caused by liraglutide were mainly concentrated in the gastrointestinal system and all of them were minor adverse events.ConclusionLiraglutide can be the drug of choice for weight management and reduction of abdominal fat distribution in patients with obesity and T2DM.
Medications and medical costs for diabetes patients with or without chronic respiratory disease in Beijing, China: A retrospective study
Authors: Qiao Jingtao , Tan Zheng , Xu Xiaomao , Zhou Yan , Wang Weihao , Luo Jingyi , Fan Jingwen , Pan Qi , Guo Lixin 
Published: August 2022

AimsThe cost of drug regimens prescribed to Chinese patients has not been evaluated. This study aims to evaluate the medical costs and hypoglycemic agents for diabetes mellitus patients with or without chronic respiratory disease in Beijing, and to investigate the changes in the costs and number of antidiabetic medications used for diabetes patients with chronic respiratory disease from 2016 to 2018.MethodsThis observational, retrospective study included diabetes patients with outpatient medication records from Beijing Medical Insurance between 2016 and 2018. The medications, including hypoglycemic and nonhypoglycemic drugs, insulin dosage, comorbidities, diabetes-related complications, treatment strategies, and annual medical costs, were recorded.ResultsThis study included 2,853,036 diabetes patients from 2016 to 2018. About 18.95%–20.53% of patients with chronic respiratory disease were predominantly distributed among those aged 45–84 years (88.7%–89.1%). Diabetes patients with chronic respiratory disease used more medications (4.48 ± 2.41 vs. 3.76 ± 2.33) and had higher total annual drug costs (¥12,286 ± 10,385 vs. ¥9700 ± 9202) to treat more comorbidities (2.52 ± 1.53 vs. 2.05 ± 1.85) than those without chronic respiratory disease (p &lt;.0001, respectively). From 2016 to 2018, diabetes patients with chronic respiratory disease had a 4.2% increase in medication, a 1.9% decrease in comorbidities, and a 5.4% decrease in total annual drug costs.ConclusionsIn summary, diabetes patients with chronic respiratory disease had more comorbidities, required more hypoglycemic drugs, and had higher medical costs. During 2016–2018, diabetes patients with chronic respiratory disease used more medications and spent less money on medical care.
MITO: A new operational model for the forecasting of the Mediterranean sea circulation
Authors: Napolitano E. , Iacono R. , Palma M. , Sannino G. , Carillo A. , Lombardi E. , Pisacane G. , Struglia M. V. 
Published: August 2022

Availability of detailed short-term forecasts of the ocean main characteristics (circulation and waves) is essential for a correct management of the human activities insisting on coastal areas. These activities include the extraction of renewable energy, which has developed in recent years, and will play an important role in the context of future blue growth. The present work describes the implementation of a new ocean operational system, named MITO, that provides daily 5 days forecasts of the Mediterranean Sea circulation. Distinctive features of this system are the inclusion of the main effects of the tidal forcing, both local and propagating from the Atlantic, and the high spatial detail. The horizontal resolution is of 1/48° (about 2 km) in most of the computational domain, and is smoothly increased (down to few hundred meters) in key passages, such as the Gibraltar Strait and the Turkish Straits, to correctly resolve the complex local dynamics. Initial and boundary conditions for MITO are taken from the reference European operation model of Copernicus, which covers the Mediterranean Sea with a uniform resolution of 1/24°. A thorough validation of the new system is performed, analyzing the forecasts of the year 2020, whose results are compared with in situ and remote observational data (sea surface temperature, altimeter data, temperature and salinity profiles by floats, tide-gauge measurements, available through the Copernicus portal) using the same large-scale metrics applied in the validation of the Copernicus operational model. MITO results are generally found in very good agreement with the observations, despite the fact that the model does not make explicit use of data assimilation. We also give examples of the capability of the model to correctly describe complex local mesoscale dynamics, and point out aspects that need to be improved, which will be addressed in a future upgrade of the operational implementation.
Insights into the anaerobic digestion of fecal sludge and food waste in Tanzania
Authors: Kilucha Malimi , Cheng Shikun , Minza Selele , Nasiruddin Sayed Mohammad , Velempini Kgosietsile , Li Xinying , Wang Xuemei , Mokeira Doroth Kerage , Li Zifu 
Published: August 2022

With the increasing demand for renewable energy and environmental protection, biogas technology has attracted considerable attention around the world. Fecal sludge (FS) is rich in organic matter, and it contains high concentrations of excreted pathogens that cause gastro-intestinal infection. In Tanzania, fecal sludge management from on-site sanitation systems poses a threat on environmental safety. This study aimed to assess the feasibility of the use of anaerobic digestion (AD) for the treatment of FS and the production of biogas as renewable energy to achieve multiple benefits in Tanzania. For the experiments, FS and food waste (FW) were used as feedstock, and rice straw-derived biochar (RSB) was added as an additive to improve biogas production. The mesophilic anaerobic digestion resulted in a methane yield of 287.5 ml/g VS for FS + FW co-digestion and 396 ml/g VS for FS + FW + RSB co-digestion. At ambient temperature (20–26°C), the system produced a methane yield of 234 ml/g VS for FS + FW co-digestion and 275 ml/g VS for FS + FW + RSB co-digestion. Three different scenarios (digester with volumes of 4, 100, and 400 m3, respectively) and strategies for FS treatment by AD in Tanzania were proposed and analyzed. These treatments can produce methane volumes of 1.95, 49.5, and 199.5 m3 with pay-back periods of 3, 5, and 15 years and net present values of + 28, +1,337, and +52,351 USD, respectively. The calculations also showed that the heat value from the produced biogas and energy needed to heat the digester at 26–37°C resulted in energy balance values of + 0.012, + 0.53, and + 2.22 GJ/day for the 4, 100, and 400 m3 digester volumes, respectively.
Green synthesis of silver nanoparticles from vegetable waste of pea Pisum sativum and bottle gourd Lagenaria siceraria: Characterization and antibacterial properties
Authors: Deepa  , Ameen Fuad , Amirul Islam M. , Dhanker Raunak 
Published: August 2022

A huge amount of food waste is being generated every day globally. Usually, India generates ∼350 million tons of food waste every year. Therefore, there is an urgent need to initiate research focusing on the management and hygienic methods of reuse of food waste together with advanced user-friendly methods of converting it into some useful products thereby generating wealth from food waste. A promising approach seems to biosynthesize silver nanoparticles (AgNPs) from such unutilized food. An alternative clean technology does not rely on the use of toxic chemicals and solvents. It is commonly allied with traditional nanoparticle synthesis processes. In the present work, the peels of two vegetables, pea (Pisum sativum) and bottle gourd (Lagenaria siceraria), were used to generate AgNPs. AgNPs were obtained by dissolving 1.5 ml of the peel extract of each vegetable in 50 ml of silver nitrate (AgNO3) and incubating for 24 h at room temperature. For the confirmation of AgNP production UV–visible spectroscopy was used. Field Emission Scanning Electron Microscopy (FESEM), X-ray diffraction (XRD), and attenuated total reflection-infrared spectroscopy (ATR-IR Analysis) were used to characterize them. Furthermore, AgNPs in different concentrations were used to test antibacterial activity against bacteria Escherichia coli through the disc diffusion method. Thus, our research indicates that AgNPs can be a safe and environmentally beneficial production technology from unutilized vegetable wastes that may play an important role in the management of food waste in the future and has antibacterial potential to preserve vegetables from bacterial contamination.
Mechanisms governing the accessibility of DNA damage proteins to constitutive heterochromatin
Authors: Roemer Anastasia , Mohammed Lanah , Strickfaden Hilmar , Underhill D. Alan , Hendzel Michael J. 
Published: August 2022

Chromatin is thought to regulate the accessibility of the underlying DNA sequence to machinery that transcribes and repairs the DNA. Heterochromatin is chromatin that maintains a sufficiently high density of DNA packing to be visible by light microscopy throughout the cell cycle and is thought to be most restrictive to transcription. Several studies have suggested that larger proteins and protein complexes are attenuated in their access to heterochromatin. In addition, heterochromatin domains may be associated with phase separated liquid condensates adding further complexity to the regulation of protein concentration within chromocenters. This provides a solvent environment distinct from the nucleoplasm, and proteins that are not size restricted in accessing this liquid environment may partition between the nucleoplasm and heterochromatin based on relative solubility. In this study, we assessed the accessibility of constitutive heterochromatin in mouse cells, which is organized into large and easily identifiable chromocenters, to fluorescently tagged DNA damage response proteins. We find that proteins larger than the expected 10 nm size limit can access the interior of heterochromatin. We find that the sensor proteins Ku70 and PARP1 enrich in mouse chromocenters. At the same time, MRE11 shows variability within an asynchronous population that ranges from depleted to enriched but is primarily homogeneously distribution between chromocenters and the nucleoplasm. While larger downstream proteins such as ATM, BRCA1, and 53BP1 are commonly depleted in chromocenters, they show a wide range of concentrations, with none being depleted beyond approximately 75%. Contradicting exclusively size-dependent accessibility, many smaller proteins, including EGFP, are also depleted in chromocenters. Our results are consistent with minimal size-dependent selectivity but a distinct solvent environment explaining reduced concentrations of diffusing nucleoplasmic proteins within the volume of the chromocenter.
The ECRG4 cleavage product augurin binds the endotoxin receptor and influences the innate immune response during otitis media
Authors: Kurabi Arwa , Hur Dong Gu , Pak Kwang , Gibson Madeline , Webster Nicholas J. G. , Baird Andrew , Eliceiri Brian P. , Ryan Allen F. 
Published: August 2022

Otitis media (OM), the most common disease of childhood, is typically characterized by bacterial infection of the middle ear (ME). Prominent features of OM include hyperplasia of the ME mucosa, which transforms from a monolayer of simple squamous epithelium with minimal stroma into a full-thickness respiratory epithelium in 2–3 days after infection. Analysis of the murine ME transcriptome during OM showed down-regulation of the tumor suppressor gene Ecrg4 that was temporally related to mucosal hyperplasia and identified stromal cells as the primary ECRG4 source. The reduction in Ecrg4 gene expression coincided with the cleavage of ECRG4 protein to release an extracellular fragment, augurin. The duration of mucosal hyperplasia during OM was greater in Ecrg4−/− mice, the number of infiltrating macrophages was enhanced, and ME infection cleared more rapidly. ECRG4-null macrophages showed increased bacterial phagocytosis. Co-immunoprecipitation identified an association of augurin with TLR4, CD14 and MD2, the components of the lipopolysaccharide (LPS) receptor. The results suggest that full-length ECRG4 is a sentinel molecule that potentially inhibits growth of the ME stroma. Processing of ECRG4 protein during inflammation, coupled with a decline in Ecrg4 gene expression, also influences the behavior of cells that do not express the gene, limiting the production of growth factors by epithelial and endothelial cells, as well as the activity of macrophages.
Identification of potential models for predicting progestin insensitivity in patients with endometrial atypical hyperplasia and endometrioid endometrial cancer based on ATAC-Seq and RNA-Seq integrated analysis
Authors: Hu Jia-Li , Yierfulati Gulinazi , Wang Lu-Lu , Yang Bing-Yi , Lv Qiao-Ying , Chen Xiao-Jun 
Published: August 2022

Objective: The aim of this study was to establish predictive models based on the molecular profiles of endometrial lesions, which might help identify progestin-insensitive endometrial atypical hyperplasia (EAH) or endometrioid endometrial cancer (EEC) patients before progestin-based fertility-preserving treatment initiation.Methods: Endometrial lesions from progestin-sensitive (PS, n = 7) and progestin-insensitive (PIS, n = 7) patients were prospectively collected before progestin treatment and then analyzed by ATAC-Seq and RNA-Seq. Potential chromatin accessibility and expression profiles were compared between the PS and PIS groups. Candidate genes were identified by bioinformatics analyses and literature review. Then expanded samples (n = 35) were used for validating bioinformatics data and conducting model establishment.Results: ATAC-Seq and RNA-Seq data were separately analyzed and then integrated for the subsequent research. A total of 230 overlapping differentially expressed genes were acquired from ATAC-Seq and RNA-Seq integrated analysis. Further, based on GO analysis, REACTOME pathways, transcription factor prediction, motif enrichment, Cytoscape analysis and literature review, 25 candidate genes potentially associated with progestin insensitivity were identified. Finally, expanded samples were used for data verification, and based on these data, three predictive models comprising 9 genes (FOXO1, IRS2, PDGFC, DIO2, SOX9, BCL11A, APOE, FYN, and KLF4) were established with an overall predictive accuracy above 90%.Conclusion: This study provided potential predictive models that might help identify progestin-insensitive EAH and EEC patients before fertility-preserving treatment.
Case Report: Novel compound heterozygous variants in CHRNA1 gene leading to lethal multiple pterygium syndrome: A case report
Authors: Zhuang Jianlong , Wang Junyu , Luo Qi , Zeng Shuhong , Chen Yu’e , Jiang Yuying , Chen Xinying , Wang Yuanbai , Xie Yingjun , Wang Gaoxiong , Chen Chunnuan 
Published: August 2022

Background: Lethal multiple pterygium syndrome (LMPS) is a rare autosomal recessive inherited disorder typically characterized by intrauterine growth retardation, multiple pterygia, and flexion contractures.Case presentation: We herein report a Chinese case with a history of three adverse pregnancies demonstrating the same ultrasonic phenotypes, including increased nuchal translucency, edema, fetal neck cystoma, reduced movement, joint contractures, and other congenital features. Whole-exome sequencing (WES) revealed novel compound heterozygous variants in the CHRNA1 gene NM_000079.4: c.[1128delG (p.Pro377LeufsTer10)]; [505T&gt;C (p.Trp169Arg)] in the recruited individual, and subsequent familial segregation showed that both parents transmitted their respective mutation.Conclusion: For the first time, we identified an association between the CHRNA1 gene and the recurrent lethal multiple pterygium syndrome (LMPS) in a Chinese family. This finding may also enrich the mutation spectrum of the CHRNA1 gene and promote the applications of WES technology in etiologic diagnosis of ultrasound anomalies in prenatal examination.
Expression of T cell-related lncRNAs in multiple sclerosis
Authors: Dadyar Maryam , Hussen Bashdar Mahmud , Eslami Solat , Taheri Mohammad , Emadi Farhad , Ghafouri-Fard Soudeh , Sayad Arezou 
Published: August 2022

Long non-coding RNAs (lncRNAs) have been demonstrated to in the pathophysiology of multiple sclerosis (MS). In order to appraise the role of T cell-related lncRNAs in this disorder, we assessed expressions of NEST, RMRP, TH2-LCR, MAFTRR and FLICR in MS patients and healthy individuals. We detected significant difference in the expression of RMRP and FLICR between cases and controls. There were substantial correlations between expressions of NEST, RMRP, TH2-LCR, MAFTRR and FLICR lncRNAs among patients, but not controls. The strongest correlations were found between RMRP and TH2-LCR, and between MAFTRR and RMRP with correlation coefficients of 0.69 and 0.59, respectively. ROC curve analysis revealed appropriate power of FLICR in differentiating between MS patients and healthy controls (AUC value = 0.84). Expression of NEST lncRNA was positively correlated with disease duration in MS patients, but negatively correlated with age at onset. In brief, we reported dysregulation of two T cell-related lncRNAs in MS patients and proposed FLICR as a putative marker for this disorder.
Comparative analysis of four types of mesoscale eddies in the Kuroshio-Oyashio extension region
Authors: Sun Wenjin , An Mengxuan , Liu Jie , Liu Jishan , Yang Jingsong , Tan Wei , Dong Changming , Liu Yu 
Published: August 2022

Oceanic mesoscale cyclonic (anticyclonic) eddies usually have cold (warm) cores and counterclockwise (clockwise) flow fields in the Northern Hemisphere. However, “abnormal” cyclonic (anticyclonic) eddies with warm (cold) cores and counterclockwise (clockwise) flow fields have recently been identified in the Kuroshio-Oyashio Extension (KOE) region. Here, traditional cyclonic cold-core eddies (CCEs) and anticyclonic warm-core eddies (AWEs) are termed normal eddies, and cyclonic warm-core eddies (CWEs) and anticyclonic cold-core eddies (ACEs) are called abnormal eddies. Applying a vector geometry-based automatic eddy detection method to the Ocean General Circulation Model for the Earth Simulator reanalysis data (OFES), a three-dimensional eddy dataset is obtained and used to quantify the statistical characteristics of these eddies. Results illustrate that the number of CCEs, AWEs, CWEs, and ACEs accounted for 38.46, 36.15, 13.40, and 11.99%, respectively. In the vertical direction, normal eddies are concentrated in the upper 2,000 m, while abnormal eddies are mainly found in the upper 600 m of the ocean. On seasonal scales, normal eddies are more abundant in winter and spring than in summer and autumn, with the opposite trend found for abnormal eddies. Potential density changes modulated by normal eddies are dominated by eddies-induced temperature anomalies, while salinity anomalies dominate the changes modulated by abnormal eddies. This study expands the types of eddies and enriches their understanding in the KOE region.