bluuebunny/crossref_metadata_2025_split huggingface dataset:
Omega-3 polyunsaturated fatty acids play roles in skeletal muscle-adipose tissue crosstalk through myokines
Authors: Wei Wenting , Zeng Huanting , Mao Limei 
Published: September 2022

Abstract
Purpose: Skeletal muscle-adipose tissue crosstalk is important for maintaining metabolic homeostasis. Myokine plays an indispensable role in the skeletal muscle-adipose tissue crosstalk. This study was conducted to elucidate the association between Omega-3 polyunsaturated fatty acids (n-3 PUFAs) and myokine and figure out the role of n-3 PUFAs in the skeletal muscle-adipose tissue crosstalk.
Methods: The mRNA expression of myokines, including IL-6, Irisin, IL-15, myostatin, FGF21, adiponectin, leptin and TNF-α, in myotube cells incubated with ALA, DHA, EPA, and DHA/EPA mixture for 24 h were evaluated. 3T3-L1 adipocytes were treated with the culture supernatant of myotube cells incubated with the DHA/EPA mixture, and the mRNA levels of genes associated with lipid metabolism and browning were evaluated. Moreover, the level of p-AMPK and PGC-1α were evaluated to determine whether AMPK signaling was involved in the process of n-3 PUFAs regulating myokines expression.
Results: Different forms of n-3 PUFAs increased the expression of multiple myokines in C2C12 myotubes. The expression of p-AMPK and PGC-1α in C2C12 myotubes were up-regulated by DHA/EPA mixture and Metformin. Except Irisin, the expression of other myokines was further up-regulated after inhibiting AMPK. The culture supernatant of myotube cells incubated with the DHA/EPA mixture regulated the lipid metabolism-related genes and stimulated browning in adipocytes.
Conclusions: n-3 PUFAs play roles in the skeletal muscle-adipose tissue crosstalk by regulating the expression and release of myokines associated with lipid metabolism and browning. Part of the mechanism of this process should be attributed to DHA/EPA activating AMPK signaling in C2C12 myotube.
Candidate biomarkers for diagnosis and prognosis of prostate cancer based on bioinformatics methods
Authors: Chen Shuchao , Li Yongshun , Ouyang Zichen , Fu Wei , Li Qixin , You Xujun 
Published: September 2022

AbstractBackground: Prostate cancer (PCa) is one of the most commonly diagnosed cancers and the fifth leading cause of cancer death in men. In this study, candidate biomarkers related to the diagnosis and prognosis of PCa were identified using bioinformatics approach.Methods: Differentially expressed genes (DEGs) between PCa tissues and matched normal tissues were screened using the R software. Enrichment analysis of the DEGs was performed to determine their functions and related pathways. PPI network was constructed, and 10 hub genes were screened using the STRING database and Cytoscape software. Weighted gene co-expression network analysis (WGCNA) was performed to extract key module genes, from which 5 key genes were identified by Venn diagram. Receiver operating characteristic (ROC) analysis was performed to identify the diagnostic value of the key genes, and their prognostic value was verified via survival analysis, which was further validated using the Human Protein Atlas.Results: We identified 661 DEGs (249 upregulated and 412 downregulated) between the PCa group and healthy controls. Overlap of PPI and WCCNA networks identified 5 key genes: BUB1B, HMMR, RRM2, CCNA2 and MELK, as candidate biomarkers for PCa. Although ROC analysis suggested that these genes had diagnostic potential in PCa, survival analysis showed that RRM2 and BUB1B were significantly associated with PCa prognosis.Conclusion: Our results showed that BUB1B, HMMR, RRM2, CCNA2 and MELK could be diagnostic biomarkers for PCa, while RRM2 and BUB1B were also associated with prognosis and could be potential therapeutic targets for PCa.
Screening of nanobody targeting alkaline phosphatase PhoX in Microcystis and detection of the PhoX in situ by fluorescence immunoassays
Authors: Zu Yao , Hong Sujuan , Yin Congcong , Luo Yu , Xu Chen , Li Jianhong 
Published: September 2022

Abstract
Cyanobacterial alkaline phosphatases (APases) play a key role in organophosphate utilization in freshwater. Tracking the distribution of APases could provide insights into the physiological response of phytoplankton to phosphorus nutrition. Extracellular APase PhoX, one of three prokaryotic APase families, is important for organophosphate utilization. Because the existing methods only give information on bulk APases activity, the specific contribution of the PhoX is hardly evaluated. To develop an immunoassay for PhoX detection, the phoX gene of Microcystis aeruginosa PCC7806 was expressed in E. coli BL21 and the purified PhoX was used as a coated antigen for screening anti-PhoX nanobodies from an alpaca antibody library. After three rounds of panning, a nanobody 3H with the highest affinity was selected. Further tests showed 3H could specifically bind to Microcystis PhoX and be used for PhoX detection by immunoblotting analysis. Then we constructed two different fluorescent-labeled 3H, EGFP-3H (with an enhanced green fluorescent protein, EGFP) and FITC-3H (with fluorescein 5-isothiocyanate, FITC), as specific fluorescent dyes for PhoX. The fluorescence staining tests with laboratory strains and water bloom samples showed that both fluorescent-labeled nanobodies could visualize the distribution and evaluate relative expression levels of PhoX in Microcystis. The nanobody 3H could be a useful regent to develop fluorescence immunoassays for in situ analyses of Microcystis PhoX.
Preparation of chitosan based antibacterial agent CS-g-DMC and its long-effective antibacterial finishing for cotton fabric
Authors: Zhang Hao , He Shiqi , Hu Yanli , Li Fengyan , Zhou Baoming 
Published: September 2022

Abstract
In this study, a chitosan-based high polymer antibacterial agent CS-g-DMC was synthesized through grafting copolymerization with an environmentally friendly initiator system of H2O2 and ascorbic acid (Vc). The CS-g-DMC was used for the long-effective antibacterial finishing of cotton fabric. The SEM, EDS, FTIR, XPS, and XRD were conducted for the characterization of the fabric. Result showed CS-g-DMC combined with cellulose macro-molecule chemical bond, and had uniformly distributed on surface of cotton fiber. After finishing, the cotton fabric had antibacterial rates against S. aureus and E. coli above 99.9% after 10 times of standard washing. The finished cotton fabric had significantly improved hydrophily, its contact angle decreased from 107° to 104°. Its breaking force significantly increased from 173.38 N to 219.33 N, and its breaking elongation and moisture transmission rate had not dropped obviously. In general, the cotton fabric finished by CS-g-DMC obtained long-effective antibacterial and increased mechanical properties, at meantime kept its comfortable capability to a great extent.
Cardiac Magnetic Resonance Feature Tracking Global and Segmental Strain in Acute and Chronic ST-elevation Myocardial Infarction
Authors: Erley Jennifer , Starekova Jitka , Sinn Martin , Muellerleile Kai , Chen Hang , Harms Phillip , Naimi Lieda , Meyer Mathias , Cavus Ersin , Schneider Jan , Blankenberg Stefan , Lund Gunnar K. , Adam Gerhard , Tahir Enver 
Published: September 2022

Abstract
Objectives: Strain is an important imaging
parameter to determine myocardial deformation. This study sought to
1) assess changes in left ventricular strain and ejection fraction
(LVEF) from acute to chronic ST-elevation myocardial infarction
(STEMI) and 2) analyze strain as a predictor of late gadolinium
enhancement (LGE).

Methods: 32 patients with STEMI and 18
controls prospectively underwent cardiac magnetic resonance
imaging. Patients were scanned 8±5 days and six months after
infarction (±1.4 months). Feature tracking was performed and LVEF
was calculated. LGE was determined visually and quantitatively on
short-axis images and myocardial segments were grouped according to
the LGE pattern (negative, non-transmural and transmural).

Results: Global strain was impaired in
patients compared to controls, but improved within six months after
STEMI (longitudinal strain from -14±4 to -16±4%, p<0.001; radial
strain from 38±11 to 42±13%, p=0.006; circumferential strain from
-15±4 to -16±4%, p=0.023). Regional strain persisted impaired in
LGE-positive segments. LVEF was preserved (51±12 and 53±10%,
p=0.206). Circumferential strain could best distinguish between
LGE-negative and -positive segments (AUC 0.73- 0.77).

Conclusion: Strain improves within six months
after STEMI, but remains impaired in LGE-positive segments. Strain
may serve as an imaging biomarker to analyze myocardial viability.
Especially circumferential strain could predict LGE.
Application of Moringa leaves as soil amendment to Tiger-nut suppressed weeds in the Nigerian Guinea Savanna
Authors: Fadeyi Olasupo James , James Olasupo , Fabunmi Thomas Oladeji , Soretire Adeniyi Adebowale , Olowe Victor Idowu Olugbenga , Adeyemi Olusegun Raphael 
Published: September 2022

Abstract
The allelopathic effect of Moringa (Moringa oleifera Lam.) leaves applied as organic manure in tiger nut (Cyperus esculentus L.) production on associated weeds was investigated in the guinea savanna of South West Nigeria, during the 2014 (September - November) and 2015 (June - August) wet seasons. Five Moringa leaves rates (0, 2.5, 5.0, 7.5 and 10 t/ha) and three tuber sizes (0.28 g, 0.49 g and 0.88 g dry weight) were laid out in the main plot and sub-plot, respectively in a split-plot arrangement fitted into randomized complete block design and replicated three times. Parameters measured, weed cover score (WCS), weed density (WD) and weed dry matter production (WDMP) were significantly (p<0.05) influenced in both years by Moringa leaf. In 2015, WCS, WD and WDMP significantly (p<0.05) reduced by 25-73%, 35-78% and 26-70% on Moringa leaves-treated plots respectively. There were significant (p<0.05) interactions between quantity of Moringa leaves incorporated and tuber size. The bigger the tuber and the higher the quantity of Moringa leaves incorporated the lower the WCS, WD and WDMP. Consequently, application of 10 t.ha-1Moringa leaves and planting of large or medium-sized tubers are recommended for optimum weed suppression in tiger nut production in South West Nigeria.
Generalized conformable fractional Newton-type method for solving nonlinear systems
Authors: Candelario Giro , Cordero Alicia , Torregrosa Juan R. , Vassileva María P. 
Published: September 2022

Abstract
In a recent paper, a conformable fractional Newton-type method was proposed for solving nonlinear equations. This method involves a lower computational cost compared to others fractional iterative methods. Indeed, the theoretical order of convergence is held in practice, and it presents a better numerical behaviour than fractional Newton-type methods formerly proposed, even compared to classical Newton-Raphson method. In this work, we design a generalization of this method for solving nonlinear systems by using a new conformable fractional Jacobian matrix, and a suitable conformable Taylor power series; and it is compared with classical Newton’s scheme. The necessary concepts and results are stated in order to design this method. Convergence analysis is made and a quadratic order of convergence is obtained, as in classical Newton's method. Numerical tests are made, and the Approximated Computational Order ofo Convergence (ACOC) supports the theory. Also, the proposed scheme shows good stability properties observed by means of convergence planes.
Characterization of qPL5, a Novel Quantitative Trait Locus (QTL) that controls Panicle Length in Rice (Oryza sativa L.)
Authors: Xu Zuopeng , Li Meng , Du Yuanyue , Li Xixu , Wang Ruixuan , Chen Zhiai , Tang Shuzhu , Liu Qiaoquan , Zhang Honggen 
Published: September 2022

Abstract
Panicle length (PL) is an important trait that determines panicle architecture and strongly affects grain yield and quality in rice. However, this trait has not been well characterized genetically, and its contribution to yield improvement is not well understood. Characterization of novel genes related to PL is of great significance for breeding high-yielding rice varieties. In our previous research we identified qPL5, a quantitative trait locus for PL. In this study, we aimed to determine the exact position of qPL5 in the rice genome and identify the candidate gene. Through substitution mapping, we mapped qPL5 to a region of 21.86 kb flanked by the molecular marker loci STS5-99 and STS5-106 in which two candidate genes were predicted. By sequence analysis and relative expression analysis, LOC-Os05g41230, which putatively encodes a BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 precursor, was considered to be the most likely candidate gene for qPL5. In addition, we successfully developed a pair of near-isogenic lines (NILs) for qPL5 in different genetic backgrounds to evaluate the genetic effects of qPL5. Agronomic trait analysis of the NILs indicated that qPL5 positively contributes to plant height, grain number per panicle, panicle length, grain yield per panicle, and flag leaf length, but it had no influence on heading date and grain-size-related traits. Therefore, qPL5 and the markers tightly linked to it should be available for molecular breeding of high-yielding varieties.
Interest Rates and Inequality: Does the Choice of Inequality Measure Matter?
Authors: Fagerstrom Matthew J. 
Published: September 2022

Abstract
Since the 1980s, interest rates have declined in many of the world's advanced economies. Concurrently, capital's share of income and both income and wealth inequality have risen. Although there is a growing literature on the relation between monetary policy and inequality, no study has yet to look at income and wealth inequality across multiple samples simultaneously. Using a century of data covering the US, France, and the UK, a panel of eight countries over the period 1960-2010, and a panel of 41 countries over the period 2000-2014, I estimate local projections and find that increases in long-term interest rates always decrease inequality regardless of how it is measured. This result is robust to the choice of country and time period. Increases in capital's share increase income inequality, but the impact of capital's share on wealth inequality is sensitive to the sample chosen.
JEL: D31, E25, E52
Efficient design of a quantum absolute-value circuit using Clifford+T gates
Authors: Orts Francisco , Ortega Gloria , Combarro Elías F. , Rúa Ignacio F. , Puertas Antonio M. , Garzón Ester M. 
Published: September 2022

Abstract
Current quantum computers have a limited number of resources and are heavily affected by internal and external noise. Therefore, small, noise-tolerant circuits are of great interest. With regard to circuit size, it is especially important to reduce the number of required qubits. Concerning to fault-tolerance, circuits entirely built with Clifford+T gates allow the use of error correction codes. However, the T-gate has an excessive cost, so circuits with a high number of T-gates should be avoided. This work focuses on optimising in such terms an operation that is widely used in larger circuits and algorithms: the calculation of the absolute-value of two's complement encoded integers. The proposed circuit reduces by more than half the number of required T gates with respect to the best circuit currently available in the literature. Moreover, our proposal is the circuit that requires the fewest qubits for such an operation.
They do not have symptoms – Why do they need to take medicines? Challenges in tuberculosis preventive treatment among children in Cambodia: a qualitative study
Authors: An Yom , Teo Alvin Kuo Jing , Huot Chan Yuda , Tieng Sivanna , Khun Kim Eam , Pheng Sok Heng , Leng Chhenglay , Deng Serongkea , Song Ngak , Nonaka Daisuke , Yi Siyan 
Published: September 2022

Abstract
Background: Latent tuberculosis (TB) infection has been known as seedbeds for TB disease later in life. The interruption from latent TB infection to TB disease can be done through TB preventive treatment (TPT). In Cambodia, only about 83.0% of children under five years old who were the household contacts to bacteriologically confirmed TB cases were initiated with TPT in 2020. Scientific studies of context-specific operational challenges in TPT provision and uptake among children are scarce, particularly in high TB-burden countries. This study identified challenges in TPT provision and uptake among children in Cambodia from the perspective of healthcare providers and caregivers.
Methods: Between October and December 2020, we conducted in-depth interviews with four operational district TB supervisors, four clinicians and four nurses in charge of TB in referral hospitals, four nurses in charge of TB in health centers, and 28 caregivers with children currently or previously on TB treatment, on TPT, and those who refused TPT for their eligible children. Data were audio recorded along with field notetaking. After verbatim transcription, data analyses were performed using a thematic approach.
Results: The mean age of healthcare providers and caregivers were 40.19 years (SD 12.0) and 47.9 years (SD 14.6), respectively. The majority of healthcare providers (93.8%) were male, and 75.0% of caregivers were female. More than one-fourth of caregivers were grandparents, and 25.0% of the caregivers had no formal education. Identified key barriers to TPT implementation among children included TPT side effects, poor adherence to TPT, poor understanding of TPT among caregivers, TPT risk perception among caregivers, child-unfriendly formula, TPT supply-chain issues, caregivers’ concern about the effectiveness of TPT, being non-parental caregivers, and poor community engagement.
Conclusion: The national TB program should invest in capacity building on TPT among healthcare providers, ensuring appropriate mechanisms to ensure adequate TPT drug supply, and improving community awareness of TPT. These context-specific interventions will play a crucial role in expanding the TPT program to interrupt the development from latent TB infection to active and ultimately lead to ending TB in the country.
Face based mental health classification
Authors: Malick Rauf A. S. , Lodhi Rubab 
Published: September 2022

Abstract
Mental Health has been the concern of multiple eras and facial expressions and gestures identification plays a vital role in mental health identification. Cognitive theories suggest people with depression interpret self-referential social information negatively. Mental Illness is curable and if treated timely it can lead to a permanent solution. Timely diagnosis is a compulsory factor and it can be done by identifying the behavior, actions and personality traits that the person possess through gestures and facial expression analysis. This can be a great help in timely diagnosis of various mental illnesses and will eventually help professionals choose suitable treatment therapies according to patient’s personality traits and level of illness. The proposed researched worked on rule based model to classify mental state from facial image data and perform comparison of results using various machine learning models over multiclass data, validating the outcome of rule based model.
Probing the allosteric modulation of P-glycoprotein: A medicinal chemistry approach towards the identification of non-competitive P-gp inhibitors.
Authors: Bonito Cátia A. , Ferreira Ricardo J. , Ferreira Maria-José. U. , Durães Fernando , Sousa Emília , Gillet Jean-Pierre , Cordeiro Maria Natália , Santos Daniel J. V. A. dos 
Published: September 2022

Abstract

A medicinal chemistry approach combining in silico and in vitro methodologies was performed aiming at identifying and characterizing putative allosteric drug-binding sites (aDBS) at the interface of the transmembrane- and nucleotide-binding domains (TMD-NBD) of P-glycoprotein. Two aDBSs were identified, one in each NBD, by means of in silico fragment-based molecular dynamics, and characterized in terms of size, polarity, and lining residues. From a small library of thioxanthone and flavanone derivatives, experimentally described to bind at the TMD-NBD interfaces, several compounds were identified to be able to decrease the verapamil-stimulated ATPase activity. An IC50 of 81 ± 6.6 μM is reported for a flavanone derivative in the ATPase assays, providing evidence for an allosteric efflux modulation in P-glycoprotein. Molecular docking and Molecular Dynamics gave additional insights on the binding mode on how flavanone derivatives may act as allosteric inhibitors.
Intermittent bursting oscillations and the bifurcation analysis in an excited Rayleigh-Duffing oscillator
Authors: Zhang Chun , Tang Qiaoxia , Wang Zhixiang 
Published: September 2022

Abstract
This paper investigates the bursting oscillations of a externally and parametrically forced Rayleigh-Duffing oscillator, in which three intermittent bursting types and one normal bursting type, namely intermittent “supHopf/supHopf-supHopf/supHopf” bursting, intermittent “fold/Homoclinic-Homoclinic/supHopf” bursting, intermittent “fold/Homoclinic-supHopf/supHopf” bursting and “fold/Homoclinic” bursting, are analyzed respectively. Recognizing the excitations as slow-varying state variables, the corresponding autonomous system can be exhibited and the bifurcation characteristics is briefly investigated, in particular, the Homoclinic bifurcation is analyzed by means of the Melnikov criterion. This paper shows that the dynamical behaviors of the excited Rayleigh-Duffing oscillator is touchy to the chosen of system parameters, different parameter conditions lead to distinct bifurcation structures that result in the trajectory approaching to different stable attractors and the appearance of different bursting forms. Our study increases the variousness of bursting oscillations and deepens the cognition of the generation mechanism of bursting dynamics. Lastly, the accuracy of the analysis presented in this paper is fully vindicated by the numerical simulations.
BP-180 can be Found in Extracellular Vesicles Derived from Blister Fluid in Bullous Pemphigoid
Authors: Gasparini Giulia , Tasso Roberta , Palamà Maria Elisabetta Federica , Ciferri Maria Chiara , Gentili Chiara , Zenzo Giovanni , Salemme Adele , Quarto Rodolfo , Parodi Aurora , Cozzani Emanuele 
Published: September 2022

Abstract
Bullous pemphigoid (BP) is an autoimmune blistering disease that targets the haemidesmosomal proteins, mainly BP180. Extracellular vesicles (EVs) have been demonstrated to carry tissue-specific autoantigens in the setting of autoimmune diseases and transplant organ rejection; this phenomenon was demonstrated to have pathogenic implications in autoimmune diseases and to correlate with transplant rejection severity.
The purpose of this study was to identify the presence of BP targeted autoantigens in blister fluid derived EVs.
We isolated, by size exclusion chromatography, EVs derived from blisters of BP-patients and from suction blisters of healthy donors. EV characterization was performed by flow cytometry and nanoparticle tracking analysis. Western blot analysis was used to investigate the presence of autoantigens.
A suspension enriched in EVs was efficiently obtained from blister fluid from patients and healthy donors. EV-enriched fractions were enriched in particles with a size distribution characterizing small-EVs (main peak was present at 94.5nm). BP180 was found, by western blot analysis, in EVs derived from blister fluid of 3 out 6 BP patients and in none of EVs isolated from suction blister fluid of healthy donors. BP230 and Dsg1 were not detectable in EVs of any of the samples. No specific clinical characteristics seemed to correlate to the presence of BP180 in EVs.
The discovery of BP180, the most important autoantigen targeted in BP, in EVs derived from blister fluid represent a milestone in the understanding of BP pathogenesis.