bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Hypothalamic-pituitary-adrenal Response to Stress among Rat
Strains: Methodological Considerations and Relevance for Neuropsychiatric
Research
Authors: Armario Antonio , Belda Xavier , Gagliano Humberto , Fuentes Silvia , Molina Patricia , Serrano Sara , Nadal Roser 
Published: December 2022

Abstract:
The hormones of the hypothalamic-pituitary-adrenal (HPA) axis, particularly glucocorticoids
(GCs), play a critical role in the behavioral and physiological consequences of exposure to
stress. For this reason, numerous studies have described differences in HPA function between different
rodent strains/lines obtained by genetic selection of certain characteristics not directly related to the
HPA axis. These studies have demonstrated a complex and poorly understood relationship between
HPA function and certain relevant behavioral characteristics. The present review first remarks important
methodological considerations regarding the evaluation and interpretation of resting and stress
levels of HPA hormones. Then, it presents works in which differences in HPA function between Lewis
and Fischer rats were explored as a model for how to approach other strain comparisons. After that,
differences in the HPA axis between classical strain pairs (e.g. High and Low anxiety rats, Roman
high- and low-avoidance, Wistar Kyoto versus Spontaneously Hypertensive or other strains, Flinder
Sensitive and Flinder Resistant lines) are described. Finally, after discussing the relationship between
HPA differences and relevant behavioral traits (anxiety-like and depression-like behavior and coping
style), an example for main methodological and interpretative concerns and how to test strain differences
is offered.
Reconfigurations of Dynamic Functional Network Connectivity in
Large-scale Brain Network after Prolonged Abstinence in Heroin Users
Authors: Zhang Shan , Yang Wenhan , Li Minpeng , Wen Xinwen , Shao Ziqiang , Li Jun , Liu Jixin , Zhang Jun , Yu Dahua , Liu Jun , Yuan Kai 
Published: December 2022

Background:
Brain recovery phenomenon after long-term abstinence had been reported in
substance use disorders. Yet, few longitudinal studies have been conducted to observe the abnormal
dynamic functional connectivity (dFNC) of large-scale brain networks and recovery after prolonged
abstinence in heroin users.

Objective:
The current study will explore the brain network dynamic connection reconfigurations after
prolonged abstinence in heroin users (HUs).

Methods:
The 10-month longitudinal design was carried out for 40 HUs. The 40 healthy controls
(HCs) were also enrolled. Group independent component analysis (GICA) and dFNC analysis were
employed to detect the different dFNC patterns of addiction-related ICNs between HUs and HCs. The
temporal properties and the graph-theoretical properties were calculated. Whether the abnormalities
would be reconfigured in HUs after prolonged abstinence was then investigated.

Results:
Based on eight functional networks extracted from GICA, four states were identified by the
dFNC analysis. Lower mean dwell time and fraction rate in state4 were found for HUs, which were
increased toward HCs after prolonged abstinence. In this state, HUs at baseline showed higher dFNC
of RECN-aSN, aSN- aSN and dDMN-pSN, which decreased after protracted abstinence. A similar recovery
phenomenon was found for the global efficiency and path length in abstinence HUs. Mean
while, the abnormal dFNC strength was correlated with craving both at baseline and after abstinence.

Conclusion:
Our longitudinal study observed the large-scale brain network reconfiguration from the
dynamic perspective in HUs after prolonged abstinence and improved the understanding of the neurobiology
of prolonged abstinence in HUs.
Bidirectional Communication Between Microglia and Astrocytes in
Neuroinflammation
Authors: Bhusal Anup , Afridi Ruqayya , Lee Won-Ha , Suk Kyoungho 
Published: December 2022

Abstract:
Neuroinflammation is a common feature of diverse nervous system pathologies. In many
instances, it begins at an early stage of the disease, paving the way for further exacerbations. The main
drivers of neuroinflammation are brain-resident glial cells, such as microglia and astrocytes. Microglia
are the primary responders to any insult to the brain parenchyma, translating the signals into diverse
molecules. These molecules derived from microglia can regulate the stimuli-dependent reactivity of
astrocytes. Once activated, astrocytes in turn, can control microglia phenotypes. Recent evidence indicates
that the crosstalk between these glial cells plays an important role in delaying or accelerating
neuroinflammation and overall disease progression. To date, various molecules have been recognized
as key mediators of the bidirectional communication between microglia and astrocytes. The current
review aims to discuss the novel molecules identified recently, which play a critical role in interglial
crosstalk, highlighting their therapeutic potential.
Noncoding RNAs: Novel Targets for Opioid Tolerance
Authors: Deng Meiling , Zou Wangyuan 
Published: December 2022

Abstract:
As a global health problem, chronic pain is one of the leading causes of disability, and it
imposes a huge economic and public health burden on families and society. Opioids represent the cornerstone
of analgesic drugs. However, opioid tolerance caused by long-term application of opioids is a
major factor leading to drug withdrawal, serious side effects caused by dose increases, and even the
death of patients, placing an increasing burden on individuals, medicine, and society. Despite efforts to
develop methods to prevent and treat opioid tolerance, no effective treatment has yet been found.
Therefore, understanding the mechanism underlying opioid tolerance is crucial for finding new prevention
and treatment strategies. Noncoding RNAs (ncRNAs) are important parts of mammalian gene
transcriptomes, and there are thousands of unique noncoding RNA sequences in cells. With the rapid
development of high-throughput genome technology, research on ncRNAs has become a hot topic in
biomedical research. In recent years, studies have shown that ncRNAs mediate physiological and
pathological processes, including chromatin remodeling, transcription, posttranscriptional modification
and signal transduction, which are key regulators of physiological processes in developmental and
disease environments and have become biomarkers and potential therapeutic targets for various diseases.
An increasing number of studies have found that ncRNAs are closely related to the development
of opioid tolerance. In this review, we have summarized the evidence that ncRNAs play an important
role in opioid tolerance and that ncRNAs may be novel targets for opioid tolerance.
Identifying TMPRSS2 Inhibitors by Drug Repurposing Screenings of
Known fXIa Inhibitors: A Computational Study
Authors: Liang Yanshu , Qiao Zhixia , Meng Fancui 
Published: December 2022

Background:
SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its
priming by TMPRSS2. Hence the inhibition of TMPRSS2 may block or decrease the severity of SARSCoV-
2, making TMPRSS2 an attractive target for COVID-19. fXIa has a similar binding pocket as
TMPRSS2, implying the possibility of fXIa inhibitors being TMPRSS2 inhibitors.

Methods:
In order to find potential TMPRSS2 inhibitors, molecular docking of known fXIa inhibitors
was performed. Molecular dynamics simulations and MM/GBSA were conducted on representative compounds
with characteristic binding modes. R-group enumeration was used to generate compounds with
better binding interactions.

Results:
Three scaffolds can make hydrogen bonds with Gly439 and Ser441, and form the chloride–
Tyr474 interactions at S1 pocket as well. Further structure optimization of one scaffold found that two
compounds have better docking scores and lower binding free energies.

Conclusion:
Compounds R1a and R1b can be taken as potentially reversible inhibitors of TMPRSS2.
Our results could provide insight into both the discovery and lead optimization of TMPRSS2 inhibitors.
Prevention of Oxidative Stress and Diseases by Antioxidant Supplementation
Authors: Martemucci Giovanni , Portincasa Piero , Centonze Vincenzo , Mariano Michele , Khalil Mohamad , D'Alessandro Angela Gabriella 
Published: December 2022

Abstract:Excessive and uncontrolled oxidative stress can damage biomacromolecules, such as lipids, proteins, carbohydrates, and DNA, by free radical and oxidant overproduction. In this review, we critically discuss the main properties of free radicals, their implications in oxidative stress, and specific pathological conditions. In clinical medicine, oxidative stress can play a role in several chronic noncommunicable diseases, such as diabetes mellitus, cardiovascular, inflammatory, neurodegenerative diseases, and tumours. Antioxidant supplements can theoretically prevent or stop the progression of diseases, but a careful literature analysis finds that more evidence is needed to dissect the ultimate beneficial effect of antioxidants versus reactive oxygen species in several diseases.
Synthesis, in silico Studies and Pharmacological Evaluation of a New
Series of Indanone Derivatives as Anti-Parkinsonian and Anti-Alzheimer’s
Agents
Authors: Bansal Ranju , Singh Ranjit , Rana Pratibha 
Published: December 2022

Objective:
Parkinson’s disease (PD) and Alzheimer’s disease (AD) are the most common
forms of neurodegenerative disorders. The aim of the current work is to study the potential of
some new indanone derivatives for the treatment of these neurological disorders.

Methods:
A new series of 4-(2-oxo-2-aminoethoxy)-2-benzylidene substituted indanone derivatives
have been synthesized and studied for anti-Parkinsonian and anti-Alzheimer’s effects. Substitution
of different aminoalkyl functionalities at the para position of 2-benzylidene moiety of
indanone ring resulted in the formation of potent anti-parkinsonian and anti-Alzheimer’s agents
(5-10). The neuroprotective effects of newly synthesized compounds were evaluated using
perphenazine (PPZ)-induced catatonia in rats and LPS-induced cognitive deficits in mice models.
Further, in silico molecular modelling studies of the new indanone derivatives were performed by
docking against the 3D structures of various neuroinflammatory mediators, such as interleukin-1β
(IL-1β), tumor necrosis factor-α (TNF-α) and monoamine oxidase-B (MAO-B), to gain the mechanistic
insights of their anti-Alzheimer’s and antiparkinsonian effects.

Results:
The newly synthesized indanone analogues 5-10 were found effective against PPZinduced
motor dysfunction and LPS-induced memory impairment in animal models. Among
all the synthesized analogues, morpholine-substituted indanone 9 displayed maximum
anti-parkinsonian activity, even better than the standard drug L-DOPA, while pyrrolidine and
piperidine substituted analogues 5 and 6 were found to be the most potent anti-Alzheimer’s
agents.

Conclusion:
The new 2-arylidene-1-indanone analogues show good potential as promising leads
for designing compounds against Parkinson’s and Alzheimer’s diseases.
Research Progress and Future Development Potential of Oridonin in
Pharmacological Activities
Authors: Jiang Jinhuan , Ye Shiying , Sun Shaowei , Cai Jiye 
Published: December 2022

Abstract:
In recent years, attention has increasingly focused on herbal medicines and their bioactive
components attributed to their multi-target pharmacological activity and low side effects.
Oridonin is a natural diterpenoid extracted from the traditional Chinese herb and is one of the
main active components of Rabdosia rubescens. Modern pharmacological studies have shown
that oridonin has anti-tumor, anti-bacterial, anti-inflammatory, anti-oxidant, cardiovascular protective,
immunomodulatory, and other effects. Based on the published literature in recent years,
we outline the pharmacological activities of oridonin, aiming to provide a theoretical basis for the
design and development of new oridonin-based drugs, as well as to facilitate the process of
oridonin for clinical use.
Tafsir atas buku “Democracy and Education” karya John Dewey oleh pendukung demokrasi
Authors: Suyato Suyato , Arpannudin Iqbal 
Published: December 2022

John Dewey’s book, Democracy and Education, published in 1916, was considered a millstone of democratic education. This article was aimed at tracing and identifying variants of interpretations among Deweyan. This article is a literature review. There are at least eight perceptions relating to Dewey’s writing on Democratic education. There are elitist, liberal, neoliberal, deliberative, multiculturalist, participative, critical, and agonistic. Each of them has a different conception, implication, suggestion, and criticism as well as debate relating to Dewey’s writing. In this article, however, just elaborates on two of them, namely liberalist and neoliberalist. Teaching Democracy has the benefit of that multi-interpretations. It is suggested that the teacher select and modify various suggestions offered by scholars of democratic education in accordance with circumstances or contextual factors in teaching democracy.
The Practice and Potential Role of HIV Self-testing in China: Systematic Review and Meta-analysis
Authors: Xiu Xiangfei , Qin Yuanyuan , Bao Yugang , Chen Yaokai , Wu Hao , Huang Xiaojie , Wang Lu 
Published: December 2022

Background
HIV self-testing (HIVST) is recommended by the World Health Organization as a valid approach to routine HIV testing services. The scale of HIVST use has gradually been expanded in China over the past 5 years. To take a closer look at the role of HIVST in China, we reviewed the promotion and application of HIVST within China.

Objective
The main objective of this study was to systematically analyze the proportion of past use and actual uptake of HIVST within China. Moreover, we aimed to quantify the effect of HIVST on HIV prevention and treatment.

Methods
In all, 5 medical databases and 2 registration systems, including PubMed, Web of Science, MEDLINE, WanFang, China National Knowledge Internet, ClinicalTrials.gov, and the Chinese Clinical Trial Registry were systematically searched for studies reporting the prevalence of HIVST use from January 1, 2010, to December 25, 2021. Meta-analyses of the pooled proportion estimates were carried out by the meta-package in R software (version 4.1.2). Statistical heterogeneity among the studies was estimated using Cochran Q test and the inconsistency index (I2).

Results
A total of 50 studies were included in our systematic review. The estimated pooled prevalence of HIVST use in China was 29.9% (95% CI 22.5%-37.9%). Among individuals who have ever used HIVST, 47.5% (95% CI 37.2%-57.8%) were tested for HIV for the first time. The pooled reactive rate of HIVST was 4.2% (95% CI 3.1%-5.8%). When HIVST revealed a reactive result, 81.3% (95% CI 70.9%-91.6%) of individuals sought medical care.

Conclusions
In recent times, HIVST has become a valuable tool for HIV prevention in China. The widespread use of HIVST in non–men who have sex with men populations needs to be endorsed and promoted. The long-term applications of HIVST and the potential consequences of self-financing of HIVST in China have yet to be explored.

Trial Registration
PROSPERO CRD42022304846; https://tinyurl.com/54d9pxy8
TOPOISOMERASE INHIBITORS ACTIVITY OF YELLOW CHAMPAKA (MICHELIA CHAMPACA L.) BARK EXTRACTS AND FRACTIONS AND ITS LIRIODENINE CONTENTS
Authors: ZUHROTUN ADE , HADISAPUTRI YUNI ELSA , ALEXANDER FREDERICK , SUSENO RUDY , WICAKSONO IMAM ADI 
Published: December 2022

Objective: This research is aimed to investigate the activity of various extracts and fractions of yellow champaka (Michelia champaca L.) by mechanism-based yeast bioassay (MBYB) against the mutant yeasts. Liriodenin content, an active compound in M. champaca, will analyze to know the correlation with this assay.
Methods: The bark of M. champaca was extracted by maceration and graded maceration using n-hexane, ethyl acetate, and methanol. The methanol extract was fractionated using water, n-hexane and ethyl acetate in the liquid-liquid extraction process. Each extract and fractions were tested in vitro by mechanism-based yeast bioassay for their topoisomerase inhibitor activity. Liriodenine content of all samples was analyzed by thin layer chromatography (TLC) densitometry method.
Results: The yeast bioassay results showed that all extracts were active as topoisomerase inhibitors (IC12 values under 8000 µg/ml) except MGM. Liriodenine can be used as a marker of active compound in ethyl acetate samples that were EAM, EAGM and EAF with Pearson analysis value-0.887 (P=0.153) and these samples were relatively more active than others.
Conclusion: This research showed that various extracts and fractions of yellow champaka bark have topoisomerase inhibitor's activity. Liriodenine can be used as a marker of the active compound so yellow champak bark is a potent natural agents for breast cancer.
Effect of Combined Bone Marrow Aspirate, Lipoaspirate, and Platelet-Rich Plasma Injections on Pain, Function, and Perceived Change Amongst Individuals with Glenohumeral Osteoarthritis
Authors: Kolber Morey , Purita Joseph , Hanney William 
Published: December 2022

Purpose: This study was conducted to investigate the efficacy of a combined minimally processed bone marrow aspirate (BMA), adipose graft, and leukocyte-rich platelet-rich plasma (PRP) intra-articular injection series on pain, function, and global rating of change (GROC) among patients with glenohumeral osteoarthritis (GHOA) and record any complications or adverse events associated with the protocol.
Methods: Ten adults (mean age 65 years) previously recalcitrant to conservative care with clinical and radiographic evidence of GHOA were included. At the initial visit, patients were assessed for eligibility of treatment. All patients were assessed pre- and post-treatment with numerical pain rating and patient-specific functional scales (PSFS). All study participants were treated with 4–6 ml of PRP, 6 ml adipose graft, and 12 ml of BMA, which were administered via a landmark-based anterior intra-articular injection. Patients were requested to return twice over 4-week intervals for booster PRP injections. At each follow-up, the GROC and prior outcome measures were completed.
Results: Patients returned after an average of 27 days for first (F1) and 68 days for the second (F2) PRP injection. Friedman Chi Square analysis indicated significant improvements in best and worse pain and PSFS from baseline initial visit to F1 and F2 (P ≤ .002). Post-hoc Wilcoxon signed-rank testing with Bonferroni correction (α = 0.017) identified significant improvements from baseline to F1 and F2 for the PSFS (P ≤ 0.012). Improvements in best and worse pain were significant at F2 (P ≤ 0.016), not F1 (P ≥ 0.02), compared to baseline. Effect sizes were large, ranging from r = 0.57 to 0.84 for pain and function. Improvements in pain, GROC, and PSFS met minimum clinically important differences at F2 based on previously validated clinimetrics. The only adverse events reported are related to administration of injectate that was temporary and managed in all cases with over-the-counter analgesics.
Conclusion: A minimally processed adipose graft with BMA and three PRP injections improved pain and function among individuals with GHOA who were recalcitrant to conservative care. Although significant functional improvement at both follow-up points occurred, clinically important and significant changes in pain did not occur until F2. A one-group design and multimodal approach limit generalization of results.
Level of Evidence: IV
Re-existência do ensino de teatro na escola básica: uma experiência de estágio supervisionado no ensino remoto
Authors: Do Nascimento Palacios Celi , Pinheiro Andrea , Oliveira Maksin 
Published: December 2022

O presente ensaio aborda a experiência do estágio supervisionado num colégio de aplicação durante o período em que estivemos trabalhando com o Ensino Remoto Emergencial. Partimos do princípio que, apesar de estarmos impedidos de trabalhar na escola física, manter o ensino de teatro em suas especificidades – considerando os limites das plataformas digitais – junto a nossos licenciandos e licenciandas era uma forma de reforçar e afirmar o lugar das Artes Cênicas no currículo da escola básica, em contraposição às agendas neoliberais e coloniais conteudistas. Partimos do conceito de re-existir, tal como proposto pelo ator e diretor teatral José Celso Martinez, como uma fusão do termos resistência e existência.Entretanto, não legitimamos a virtualidade como uma possibilidade futura para a Educação, principalmente, a Educação Básica. Acreditamos que era preciso explorar a plataforma digital naquele momento emergencial para manter a arte teatral no cotidiano educacional de jovens e crianças. Entendemos que escola é convívio e construção de alteridade e que o teatro é arte do presente e da presença.
Diversity and independent evolutionary profiling of rodent-borne pathogens in tropical island, China
Authors: Li Youyou , Tang Chuanning , Zhang Yun , Li Zihan , Wang Gaoyu , Peng Ruoyan , Huang Yi , Hu Xiaoyuan , Xin Henan , Feng Boxuan , Cao Xuefang , He Yongpeng , Guo Tonglei , He Yijun , Su Haoxiang , Cui Xiuji , Niu Lina , Wu Zhiqiang , Yang Jian , Yang Fan , Lu Gang , Gao Lei , Jin Qi , Xiao Meifang , Yin Feifei , Du Jiang 
Published: December 2022

The risk of emerging infectious diseases (EID) is increasing globally.
More than 60% of EIDs worldwide are caused by animal-borne pathogens,
and most viral pathogens are rodent-borne. This study aimed to
characterise the virome and analyse the phylogenetic evolution and
diversity of rodent-borne viruses in Hainan Province, China. We
collected 588 anal and throat samples from rodents, combined them into
28 pools according to their species and location, and processed them for
next-generation sequencing and bioinformatics analysis. The diverse
viral reads closely related to mammals were assigned to 15 viral
families. Molecular clues of the important rodent-borne viruses were
further identified by polymerase chain reaction for phylogenetic
analysis and annotation of genetic characteristics such as coronavirus,
arenavirus, picornavirus. We identified a pestivirus in Leopoldoms
edwardsi and two bocaviruses in Rattus andamanensis and Leopoldoms
edwardsi from the national nature reserves of Jianfengling and Bangxi
with low amino acid identity to known pathogens are proposed as the
novel species, and their rodent hosts have not been previously reported
to carry these viruses. These results expand our knowledge of viral
classification and host range and suggest that there are highly diverse,
undiscovered viruses that have evolved independently in their unique
wildlife hosts in inaccessible areas, which may cause zoonosis if they
cross their host barrier. Our virome and phylogenetic analyses of
rodent-borne viruses provide basic data for the prevention and control
of human infectious diseases caused by rodent-borne viruses in the
subtropical area of China.
Girded about with a Lambskin
Authors: Brown Matthew B. 
Published: December 2022

Abstract
The publication of the Book of Mormon brought forward the first of many comparisons between the restorational work of the Prophet Joseph Smith and his surrounding environment, including Freemasonry. One point of comparison has been the lambskin apparel mentioned in 3 Nephi 4:7. I will suggest a possible connection between this item of apparel and ritual clothing that was worn in ancient Israel, Egypt, and Mesoamerica. I will also suggest a possible reason for the use of this item of clothing among the secret combinations in the Book of Mormon. Finally, I will discuss the lambskin apron used in Freemasonic ritual.