bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Injection Pressure on Spray Cone and Penetration Angle for Enhanced Fuel Atomization of Various Blended Viscous Fluid: A Numerical Modeling
Authors: Arunkumar G. , Dhavare Prashant , Alharbi Sulaiman Ali , Nasif Omaima , Strunecky Otakar , Subramani Nithya 
Published: May 2022

Abstract
The use of the high viscous biofuel results in poor combustion efficiency. Utilization of the biofuel on the existing engine is challenging due to the higher fuel pump force requirement and atomization effects. Hence, in this study, the spray characteristics have been examined in addition to the typical combustion and emission characteristics. In general, spray properties changes are based on the viscosity of the fuel used. Utilizing the higher viscous fluid in the engine creates havoc on liquid penetration and vapor penetration. A series of tests was conducted in the single-cylinder four-stroke diesel engine fuelled with Jatropha Curcas. The biodiesel blends were prepared at three different combinations of 10%, 20%, and 30% dispersed with the Fe2O3 nanoparticles at 50 ppm to form JF10 (10% blend + 90% diesel with 50 ppm of Fe2O3), JF20 (20% blend + 80% diesel with 50 ppm of Fe2O3), and JF30 (30% blend + 70% diesel with 50 ppm of Fe2O3). Based on the previous study observation, adding the blends affects the both combustion and performance of the engine which is counteracted in this study by adding the nanoparticles to the blends. From the obtained results, it is proved that the addition of nanoparticles increases the engine performance and emission characteristics. To be precise, the brake thermal efficiency has been improvised by 4% for the JF10 compared to neat diesel. With regard to emissions, a massive reduction in CO and NOx has been observed. To understand the quality of combustion, the fluid spray simulation has been carried out. A set of numerical simulations were done using the particle droplet analysis with the aid of star ccm+ and found that injection pressure and ambient pressure are the key responsible parameters for increasing the combustion efficiency of the system. On the other hand, the liquid length of blended fuel is another key factor that affects the atomization process. Furthermore, the high injection pressure reduces the spray cone angle for biodiesel by achieving high mixing rates.
ActiGraph Cutpoints Impact Physical Activity and Sedentary Behavior Outcomes in Young Children
Authors: Breau Becky , Coyle-Asbil Hannah J. , Haines Jess , Ma David W.L. , Vallis Lori Ann , _ _ 
Published: May 2022

Purpose: Examine the effect of cutpoint selection on physical activity (PA) metrics calculated from young children’s accelerometer data and on the proportion of children meeting PA guidelines. Methods: A total of 262 children (3.6 ± 1.4 years, 126 males) wore ActiGraph wGT3X-BT accelerometers on their right hip for 7 days, 24 hr/day. Ten cutpoint sets were applied to the sample categorized by age, based on populations of the original cutpoint calibration studies using ActiLife software. Resulting sedentary behavior, light PA, moderate to vigorous PA, and total PA were compared using repeated-measures analysis of variance. Proportion of children meeting age-appropriate PA guidelines based on each cutpoint set was assessed using Cochran’s q tests. Results: Children wore the accelerometer for an average of 7.6 ± 1.2 days for an average of 11.9 ± 1.2 hr/day. Significant differences in time spent in each intensity were found across all cutpoints except for sedentary, and total PA for three comparisons (Trost vs. Butte Vertical Axis [VA], Pate vs. Puyau, and Costa VA vs. Evenson) and moderate to vigorous PA for four comparisons (Trost vs. Pate, Trost vs. Pate and Pfeiffer, Pate vs. Pate and Pfeiffer, and van Cauwenberghe vs. Evenson). When examined within age-appropriate groups, all sets of cutpoints resulted in significant differences across all intensities and in the number of children meeting PA guidelines. Conclusion: Choice of cutpoints applied to data from young children significantly affects times calculated for different movement intensities, which in turn impacts the proportion of children meeting guidelines. Thus, comparisons of movement intensities should not be made across studies using different sets of cutpoints.
Comparison of Child and Adolescent Physical Activity Levels From Open-Source Versus ActiGraph Counts
Authors: Clevenger Kimberly A. , Mackintosh Kelly A. , McNarry Melitta A. , Pfeiffer Karin A. , Montoye Alexander H.K. , Brønd Jan Christian 
Published: May 2022

ActiGraph counts are commonly used for characterizing physical activity intensity and energy expenditure and are among the most well-studied accelerometer metrics. Researchers have recently replicated the counts processing method using a mechanical setup, now allowing users to generate counts from raw acceleration data. Purpose: The purpose of this study was to compare ActiGraph-generated counts to open-source counts and assess the impact on free-living physical activity levels derived from cut points, machine learning, and two-regression models. Methods: Children (n = 488, 13.0 ± 1.1 years of age) wore an ActiGraph wGT3X-BT on their right hip for 7 days during waking hours. ActiGraph counts and counts generated from raw acceleration data were compared at the epoch-level and as overall means. Seven methods were used to classify overall and epoch-level activity intensity. Outcomes were compared using weighted kappa, correlations, mean absolute deviation, and two one-sided equivalence testing. Results: All outcomes were statistically equivalent between ActiGraph and open-source counts; weighted kappa was ≥.971 and epoch-level correlations were ≥.992, indicating very high agreement. Bland–Altman plots indicated differences increased with activity intensity, but overall differences between ActiGraph and open-source counts were minimal (e.g., epoch-level mean absolute difference of 23.9 vector magnitude counts per minute). Regardless of classification model, average differences translated to 1.4–2.6 min/day for moderate- to vigorous-intensity physical activity. Conclusion: Open-source counts may be used to enhance comparability of future studies, streamline data analysis, and enable researchers to use existing developed models with alternative accelerometer brands. Future studies should verify the performance of open-source counts for other outcomes, like sleep.
Modeling the Structure of Human tRNA-Guanine Transglycosylase in Complex with 7-Methylguanine and Revealing the Factors that Determine the Enzyme Interaction with Inhibitors
Authors: Pushkarev Sergey V. , Vinnik Valeriia A. , Shapovalova Irina V. , Švedas Vytas K. , Nilov Dmitry K. 
Published: May 2022

Abstract
tRNA-guanine transglycosylase, an enzyme catalyzing replacement of guanine with queuine in human tRNA and participating in the translation mechanism, is involved in the development of cancer. However, information on the small-molecule inhibitors that can suppress activity of this enzyme is very limited. Molecular dynamics simulations were used to determine the amino acid residues that provide efficient binding of inhibitors in the active site of tRNA-guanine transglycosylase. It was demonstrated using 7-methylguanine molecule as a probe that the ability of the inhibitor to adopt a charged state in the environment of hydrogen bond acceptors Asp105 and Asp159 plays a key role in complex formation. Formation of the hydrogen bonds and hydrophobic contacts with Gln202, Gly229, Phe109, and Met259 residues are also important. It has been predicted that introduction of the substituents would have a different effect on the ability to inhibit tRNA-guanine transglycosylase, as well as the DNA repair protein poly(ADP-ribose) polymerase 1, which can contribute to the development of more efficient and selective compounds.
Effects of the COVID-19 pandemic on stroke response times: a systematic review and meta-analysis
Authors: Nawabi Noah L A , Duey Akiro H , Kilgallon John L , Jessurun Charissa , Doucette Joanne , Mekary Rania A , Aziz-Sultan Mohammad Ali 
Published: May 2022

ObjectivesCOVID-19 presents a risk for delays to stroke treatment. We examined how COVID-19 affected stroke response times.MethodsA literature search was conducted to identify articles covering stroke during COVID-19 that included time metrics data pre- and post-pandemic. For each outcome, pooled relative change from baseline and 95% CI were calculated using random-effects models. Heterogeneity was explored through subgroup analyses comparing comprehensive stroke centers (CSCs) to non-CSCs.Results38 included studies reported on 6109 patients during COVID-19 and 14 637 patients during the pre-COVID period. Pooled increases of 20.9% (95% CI 5.8% to 36.1%) in last-known-well (LKW) to arrival times, 1.2% (−2.9% to 5.3%) in door-to-imaging (DTI), 0.8% (–2.9% to 4.5%) in door-to-needle (DTN), 2.8% (−5.0% to 10.6%) in door-to-groin (DTG), and 19.7% (11.1% to 28.2%) in door-to-reperfusion (DTR) times were observed during COVID-19. At CSCs, LKW increased by 24.0% (−0.3% to 48.2%), DTI increased by 1.6% (−3.0% to 6.1%), DTN increased by 3.6% (1.2% to 6.0%), DTG increased by 4.6% (−5.9% to 15.1%), and DTR increased by 21.2% (12.3% to 30.1%). At non-CSCs, LKW increased by 12.4% (−1.0% to 25.7%), DTI increased by 0.2% (−2.0% to 2.4%), DTN decreased by −4.6% (−11.9% to 2.7%), DTG decreased by −0.6% (−8.3% to 7.1%), and DTR increased by 0.5% (−31.0% to 32.0%). The increases during COVID-19 in LKW (p=0.01) and DTR (p=0.00) were statistically significant, as was the difference in DTN delays between CSCs and non-CSCs (p=0.04).ConclusionsFactors during COVID-19 resulted in significantly delayed LKW and DTR, and mild delays in DTI, DTN, and DTG. CSCs experience more pronounced delays than non-CSCs.
Endovascular thrombectomy versus standard medical treatment for stroke patients with acute basilar artery occlusion: a systematic review and meta-analysis
Authors: Zhao Yongli , Zhao Wendong , Guo Yingchang , Li Yanru 
Published: May 2022

BackgroundWhether endovascular thrombectomy (EVT) is superior to standard medical treatment (SMT) for stroke patients with acute basilar artery occlusion (BAO) is uncertain. This systematic review and meta-analysis aimed to compare the safety and efficacy of EVT with SMT for treating BAO patients.MethodsPapers were retrieved from PubMed, Embase, and the Cochrane Library databases. The primary outcome of this meta-analysis was favorable functional outcomes at 3 months (defined as a modified Rankin Scale (mRS) score of ≤3). A random effect model was used to calculate risk ratios (RR) with 95% confidence intervals (CIs) per outcome.ResultsFive articles, including two randomized controlled trials (RCTs) and four observational cohort studies, comprising 1484 patients (1024 in the EVT group and 460 in the SMT group), were included in the meta-analysis. The pooled results demonstrated no significant differences between the EVT and SMT groups in achieving favorable functional outcomes at 3 months (RR=1.63, 95% CI 0.90, 2.96; p=0.11). However, patients in the EVT group had higher rates for symptomatic intracerebral hemorrhage (RR=6.22, 95% CI 2.06 to 18.76; p=0.001) but lower mortality at 3 months (RR=0.72, 95% CI 0.56 to 0.91; p=0.007) than patients in the SMT group.ConclusionAmong patients with BAO, EVT and SMT did not differ significantly in achieving favorable functional outcomes at 3 months, but BAO patients treated with EVT might have lower mortality at 3 months. RCTs are warranted to further assess the efficacy and safety of EVT for BAO patients.
Thrombectomy in basilar artery occlusions: impact of number of passes and futile reperfusion
Authors: de Havenon Adam , Elhorany Mahmoud , Boulouis Gregoire , Naggara Olivier , Darcourt Jean , Clarençon Frédéric , Richard Sébastien , Marnat Gaultier , Bourcier Romain , Sibon Igor , Arquizan Caroline , Dargazanli Cyril , Maïer Benjamin , Seners Pierre , Lapergue Bertrand , Consoli Arturo , Eugene Francois , Vannier Stephane , Caroff Jildaz , Denier Christian , Boulanger Marion , Gauberti Maxime , Rouchaud Aymeric , Macian Francisco , Rosso Charlotte , Turc Guillaume , Ozkul-Wermester Ozlem , Papagiannaki Chrisanthi , Albucher Jean François , Le Bras Anthony , Evain Sarah , Wolff Valerie , Pop Raoul , Timsit Serge , Gentric Jean-Christophe , Bourdain Frédéric , Veunac Louis , Fahed Robert , Finitsis Stephanos Nikolaos , Gory Benjamin 
Published: May 2022

BackgroundThe number of mechanical thrombectomy (MT) passes is strongly associated with angiographic reperfusion as well as clinical outcomes in patients with anterior circulation ischemic stroke. However, these associations have not been analyzed in patients with basilar artery occlusion (BAO). We investigated the influence of the number of MT passes on the degree of reperfusion and clinical outcomes, and compared outcome after ≤3 passes versus >3 passes.MethodsWe used data from the prospective multicentric Endovascular Treatment in Ischemic Stroke (ETIS) Registry at 18 sites in France. Patients with BAO treated with MT were included. The primary outcome was a favorable outcome, defined as a modified Rankin Scale score of 0–3 at 90 days. We fit mixed multiple regression models, with center as a random effect.ResultsWe included 275 patients. Successful recanalization (modified Thrombolysis In Cerebral Infarction (mTICI) 2b-3) was achieved in 88.4%, and 41.8% had a favorable outcome. The odds ratio for favorable outcome with each pass above 1 was 0.41 (95% CI 0.23 to 0.73) and for recanalization (mTICI 2b-3) it was 0.70 (95% CI 0.57 to 0.87). In patients with ≤3 passes, the rate of favorable outcome in recanalized versus non-recanalized patients was 50.5% versus 10.0% (p=0.001), while in those with >3 passes it was 16.7% versus 15.2% (p=0.901).ConclusionsWe found that BAO patients had a significant relationship between the number of MT passes and both recanalization and favorable functional outcome. We further found that the benefit of recanalization in BAO patients was significant only when recanalization was achieved within three passes, encouraging at least three passes before stopping the procedure.
Treatment of wide-necked bifurcation aneurysms with the eCLIPs device: 5-year experience of a single center
Authors: Diestro Jose Danilo Bengzon , Keough Michael B , Ashforth Robert A , Chow Michael M , Rempel Jeremy L , Marotta Thomas R , O'Kelly Cian 
Published: May 2022

BackgroundThe endovascular clip system (eCLIPs) is a novel device with both neck bridging and flow-diversion properties that make it suitable for the treatment of wide-necked bifurcation aneurysms.ObjectiveTo describe the clinical and radiologic outcomes of the eCLIPs device, including the first-in-man use of the latest version of the device.MethodsThis is a retrospective case series on all the wide-necked bifurcation aneurysms treated with the eCLIPs device in our center. The immediate and latest radiologic and clinical outcomes were assessed.ResultsThe device was successfully implanted in 12 of 13 patients. After a median follow-up period of 19 months (range 3–64 months), all patients with available data (11/12) had a good radiologic outcome (modified Raymond-Roy classification scores of 1 or 2). Two patients (18.2%) underwent re-treatment with simple coiling through the device. One of these had a subarachnoid hemorrhage prior to re-treatment. There were no major complications (death or permanent neurologic deficits) associated with use of the device.ConclusionOur series demonstrates occlusion rates that are similar to those of standard stent-assisted coiling and intrasaccular flow diversion for wide-necked bifurcation aneurysms. Larger registry-based studies are necessary to support our findings.
Morphological characteristics associated with ruptured intracranial vertebral artery dissecting aneurysms
Authors: Han Jiangli , Chen Jigang , Tong Xin , Han Mingyang , Peng Fei , Niu Hao , Liu Lang , Liu Fei , Liu Aihua 
Published: May 2022

ObjectiveMorphological risk factors for the rupture of intracranial vertebral artery dissecting aneurysms (IVADAs) have not been well characterized. In this study, we aim to identify morphological characteristics associated with IVADA rupture.MethodsWe conducted a retrospective study of 249 consecutive patients with single IVADAs (31 ruptured and 218 unruptured) admitted to Beijing Tiantan Hospital between January 2016 and December 2020. Various morphological parameters were measured using three-dimensional digital subtraction angiography images. Univariate and multivariate logistic regression analyses were performed to identify morphological characteristics associated with IVADA rupture.ResultsUnivariate regression analysis revealed that the coexistence of significant proximal and distal stenosis and posterior inferior cerebellar artery (PICA) involvement were associated with IVADA rupture, while the origin from the dominant vertebral artery was inversely associated with the rupture. Multivariate regression analysis demonstrated that the coexistence of significant proximal and distal stenosis (OR 22.00, 95% CI 5.60 to 86.70, p<0.001) and PICA involvement (OR 4.55, 95% CI 1.36 to 15.20, p=0.014) were independently associated with IVADA rupture.ConclusionThe coexistence of significant proximal and distal stenosis and PICA involvement were independently associated with IVADA rupture. These morphological characteristics may facilitate the assessment of rupture risk in patients with IVADAs.
Capture Rate of V(D)J Sequencing for Minimal Residual Disease Detection in Multiple Myeloma
Authors: Hultcrantz Malin , Rustad Even H. , Yellapantula Venkata , Jacob Allison , Akhlaghi Theresia , Korde Neha , Mailankody Sham , Lesokhin Alexander M. , Hassoun Hani , Smith Eric L. , Lahoud Oscar B. , Landau Heather J. , Shah Gunjan L. , Scordo Michael , Chung David J. , Giralt Sergio , Papaemmanuil Elli , Landgren Ola 
Published: May 2022

Abstract

Purpose:
Minimal residual disease (MRD) negativity is a strong predictor for outcome in multiple myeloma. To assess V(D)J clonotype capture using the updated Adaptive next-generation sequencing (NGS) MRD assay in a clinical setting, we analyzed baseline and follow-up samples from patients with multiple myeloma who achieved deep clinical responses.

Experimental Design:
A total of 159 baseline and 31 follow-up samples from patients with multiple myeloma were sequenced using the NGS MRD assay. Baseline samples were also sequenced using a targeted multiple myeloma panel (myTYPE). We estimated ORs with 95% confidence intervals (CI) for clonotypes detection using logistic regression.

Results:
The V(D)J clonotype capture rate was 93% in baseline samples with detectable genomic aberrations, indicating presence of tumor DNA, assessed through myTYPE. myTYPE-positive samples had significantly higher V(D)J clonotype detection rates in univariate (OR, 7.3; 95% CI, 2.8–22.6) and multivariate analysis (OR, 4.4; 95% CI, 1.4–16.9; P = 0.016). Higher disease burden was associated with higher probability of V(D)J clonotype capture, meanwhile no such association was found for age, gender, or type of heavy or light immunoglobulin chain. All V(D)J clonotypes detected at baseline were detected in MRD-positive samples indicating that the V(D)J clonotypes remained stable and did not undergo further rearrangements during follow-up. Of the 31 posttreatment samples, 12 were MRD-negative using the NGS MRD assay.

Conclusions:
NGS for V(D)J rearrangements in multiple myeloma offers a reliable and sensitive method for MRD tracking with high detection rates in the clinical setting.
Antitumor Activity of a Mitochondrial-Targeted HSP90 Inhibitor in Gliomas
Authors: Wei Shiyou , Yin Delong , Yu Shengnan , Lin Xiang , Savani Milan R. , Du Kuang , Ku Yin , Wu Di , Li Shasha , Liu Hao , Tian Meng , Chen Yaohui , Bowie Michelle , Hariharan Seethalakshmi , Waitkus Matthew , Keir Stephen T. , Sugarman Eric T. , Deek Rebecca A. , Labrie Marilyne , Khasraw Mustafa , Lu Yiling , Mills Gordon B. , Herlyn Meenhard , Wu Kongming , Liu Lunxu , Wei Zhi , Flaherty Keith T. , Abdullah Kalil , Zhang Gao , Ashley David M. 
Published: May 2022

Abstract

Purpose:
To investigate the antitumor activity of a mitochondrial-localized HSP90 inhibitor, Gamitrinib, in multiple glioma models, and to elucidate the antitumor mechanisms of Gamitrinib in gliomas.

Experimental Design:
A broad panel of primary and temozolomide (TMZ)-resistant human glioma cell lines were screened by cell viability assays, flow cytometry, and crystal violet assays to investigate the therapeutic efficacy of Gamitrinib. Seahorse assays were used to measure the mitochondrial respiration of glioma cells. Integrated analyses of RNA sequencing (RNAseq) and reverse phase protein array (RPPA) data were performed to reveal the potential antitumor mechanisms of Gamitrinib. Neurospheres, patient-derived organoids (PDO), cell line–derived xenografts (CDX), and patient-derived xenografts (PDX) models were generated to further evaluate the therapeutic efficacy of Gamitrinib.

Results:
Gamitrinib inhibited cell proliferation and induced cell apoptosis and death in 17 primary glioma cell lines, 6 TMZ-resistant glioma cell lines, 4 neurospheres, and 3 PDOs. Importantly, Gamitrinib significantly delayed the tumor growth and improved survival of mice in both CDX and PDX models in which tumors were either subcutaneously or intracranially implanted. Integrated computational analyses of RNAseq and RPPA data revealed that Gamitrinib exhibited its antitumor activity via (i) suppressing mitochondrial biogenesis, OXPHOS, and cell-cycle progression and (ii) activating the energy-sensing AMP-activated kinase, DNA damage, and stress response.

Conclusions:
These preclinical findings established the therapeutic role of Gamitrinib in gliomas and revealed the inhibition of mitochondrial biogenesis and tumor bioenergetics as the primary antitumor mechanisms in gliomas.
TGF-β-Induced FLRT3 Attenuation Is Essential for Cancer-Associated Fibroblast–Mediated Epithelial–Mesenchymal Transition in Colorectal Cancer
Authors: Yang Mengdi , Li Dan , Jiang Zhiyuan , Li Changcan , Ji Suyuan , Sun Jing , Chang Yujie , Ruan Shunyi , Wang Zhiyu , Liang Rui , Dai Xueyu , Li Bin , Zhao Hui 
Published: May 2022

Abstract

Cancer-associated fibroblasts (CAF) constitute a major component of the tumor microenvironment. The effects of CAFs on the progression of colorectal cancer remain controversial. In this study, we found the ectopic overexpression of Fibronectin leucine-rich transmembrane protein 3 (FLRT3) inhibited the process of epithelial–mesenchymal transition (EMT), as well as the proliferation, migration, invasion, and promote apoptosis of colorectal cancer cells, whereas silencing FLRT3 expression resulted in the opposite phenomenon. FLRT3 downregulation was associated with a poor prognosis in colorectal cancer. Also, FLRT3 expression was significantly related to some clinicopathologic factors, including T stage (P = 0.037), N stage (P = 0.042), and E-cadherin (P = 0.002) level. Via univariate and multivariate analyses, M stage (P &lt; 0.0001), FLRT3 (P = 0.044), and E-cadherin (P = 0.003) were associated with overall survival and were independent prognostic factors for it. Mechanistically, CAFs secreted TGF-β, which downregulated FLRT3 expression by activating SMAD4 to promote aggressive phenotypes in colorectal cancer cells. Moreover, FLRT3 repressed tumorigenesis and lung metastasis, which could be reversed by LY2109761, a dual inhibitor of TGF-β receptor type I and II. Treatment with LY2109761 increased IFN-γ expression in CD8+ T cells and reduced the number of regulatory T cells in the tumor microenvironment. Taken together, we revealed the metastasis-suppressive function of FLRT3, which was attenuated during the CAFs-mediated activation of the TGF-β/SMAD4 signaling pathway to promote EMT in colorectal cancer. LY2109761 that significantly inhibited metastasis could be a new treatment option for advanced colorectal cancer.

Implications:
CAFs enhance colorectal cancer aggressiveness by reducing FLRT3 expression through activating TGF-β/SMAD4 signaling pathway. CAF-targeted therapy and/or LY2109761 were promising treatments for colorectal cancer.