bluuebunny/crossref_metadata_2025_split huggingface dataset:
Workplace Factors Associated With Return to Work After Mild-to-Moderate Traumatic Brain Injury
Authors: Fure Silje Christine Reistad , Howe Emilie Isager , Andelic Nada , Brunborg Cathrine , Olsen Alexander , Rike Per-Ola , Spjelkavik Øystein , Enehaug Heidi , Røe Cecilie , Løvstad Marianne 
Published: March 2022

Objective:
Sociodemographic and injury-related predictors for return to work (RTW) after mild-to-moderate traumatic brain injury (TBI) have been extensively explored. However, there is a knowledge gap regarding work-related predictors of RTW. The main aim of this study was to explore work-related predictors of work participation 6 and 12 months after mild-to-moderate TBI.

Setting:
Data were collected at baseline 8 to 12 weeks after injury, and 3, 6, and 12 months after baseline, at a specialized TBI rehabilitation outpatient clinic at Oslo University Hospital, Oslo, Norway.

Participants:
Eligible patients had suffered a mild-to-moderate TBI 8 to 12 weeks previously, were employed 50% or more at time of injury, were between 18 and 60 years of age, and sick listed 50% or more at time of inclusion due to symptoms of TBI (based on the Rivermead Post-Concussion Symptoms Questionnaire). In total, 116 patients were included in a randomized controlled trial, of whom 113 were included in the 1-year analysis.

Design:
Patients were originally included in a randomized controlled trial. There were no between-group differences in RTW after 1 year. Thus, the participants were evaluated as one cohort in this study.

Main Measures:
The primary outcome measure was work participation 1 year after study inclusion. Work-related predictors were chosen on the basis of previous research and expert opinion and entered into a multivariable linear regression model. The model controlled for sociodemographic and injury-related factors.

Results:
The best-fitting model explained 25% of variation in work participation at 1 year. Significant predictors were predictability, quantitative demands and rewards (recognition) at the workplace, private or public employment, symptom burden at baseline, and sex.

Conclusion:
In this study, several work-related predictors outperformed some of the established sociodemographic and injury-related predictors of RTW after TBI, thus stressing the need for further focus and research on amendable predictors of RTW after mild-to-moderate TBI.
Variation in Usual Source of Care in Asian American, Native Hawaiian, and Other Pacific Islander Adult Medicaid Beneficiaries
Authors: Nguyen Kevin H. , Oh Eunhae G. , Trivedi Amal N. 
Published: March 2022

Background:
Disparities in access to care between non-Hispanic White and Asian American, Native Hawaiian, and Pacific Islander (AANHPI) patients are often attributed to higher uninsurance rates among AANHPI patients. Less is known about variation among individuals with Medicaid health insurance coverage and among AANHPI subgroups.

Objective:
The objective of this study was to examine differences in access to care between White and AANHPI adult Medicaid beneficiaries, both in the aggregate and disaggregated into 9 ethnic subgroups (Asian Indian, Chinese, Filipino, Japanese, Korean, Vietnamese, Other Asian, Native Hawaiian, and Pacific Islander).

Research Design:
Nationwide Adult Medicaid Consumer Assessment of Healthcare Providers and Systems data (2014–2015), a cross-sectional survey representative of all Medicaid beneficiaries.

Subjects:
A total of 126,728 White and 10,089 AANHPI Medicaid beneficiaries were included.

Measures:
The study outcomes were: (1) having a usual source of care; and (2) reporting a health center or clinic as the usual site of care. Multivariable linear probability models assessed the relationship between race/ethnic subgroup and our outcomes, adjusting for sociodemographic characteristics and health status.

Results:
Compared with White beneficiaries, Korean beneficiaries were significantly less likely to report having a usual source of care [difference=−8.9 percentage points (PP), P=0.01], and Chinese (difference=8.4 PP, P=0.001), Native Hawaiian (difference=25.8 PP, P<0.001), and Pacific Islander (difference=22.2 PP, P=0.001) beneficiaries were significantly more likely to report a health center or clinic as their usual site of care.

Conclusions:
Despite similar health insurance coverage, significant differences in access to care remain between White and AANHPI Medicaid beneficiaries. Disaggregated AANHPI data may reveal important variation in access to care and inform more targeted public policies.
Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
Authors: Henn Dominic , Zhao Dehua , Chen Kellen , Trotsyuk Artem , Bonham Clark Andrew , Fischer Katharina S. , Kehl Tim , Fehlmann Tobias , Sivaraj Dharshan , Greco Autumn H. , Moortgat Illouz Sylvia E. , Padmanabhan Jagannath , Barrera Janos A. , Kneser Ulrich , Lenhof Hans-Peter , Januszyk Michael , Levi Benjamin , Keller Andreas , Longaker Michael T. , Qi Lei S. , Gurtner Geoffrey C. 
Published: March 2022

AbstractChronic wounds impose a significant healthcare burden to a broad patient population. Cell based therapies, while having shown benefits for the treatment of chronic wounds, have not achieved widespread adoption into clinical practice. Here, we developed a novel CRISPR/Cas9 approach to precisely edit dendritic cells (DCs) to enhance their therapeutic potential for healing chronic wounds. Using single-cell RNA sequencing (scRNA-seq) of tolerogenic DCs, we discover N-myc downregulated gene 2 (Ndrg2), which marks a specific population of DC progenitors, as a promising target for CRISPR knockout (KO). Ndrg2-KO alters the transcriptomic profile of DCs and preserves an immature cell state with a strong, pro-angiogenic and regenerative capacity. We then incorporated our CRISPR-based cell engineering within a hydrogel technology for in vivo cell delivery and developed a highly effective translational approach for DC based immunotherapy that accelerated healing of full-thickness wounds in both non-diabetic and diabetic mouse models. These findings could open the door to future clinical trials using safe gene editing in DCs for treating various types of chronic wounds.
A pathogenicity locus of Streptococcus gallolyticus subspecies gallolyticus
Authors: Taylor John Culver , Kumar Ritesh , Xu Juan , Xu Yi 
Published: March 2022

AbstractStreptococcus gallolyticus subspecies gallolyticus (Sgg) is known to be strongly associated with colorectal cancer (CRC). Recent functional studies further demonstrated that Sgg actively stimulates CRC cell proliferation and promotes the development of colon tumors. However, the Sgg factors important for the pro-proliferative and pro-tumor activities of Sgg remain unclear. Here, we identified a chromosomal locus in Sgg strain TX20005. Deletion of this locus significantly reduced Sgg adherence to CRC cells and abrogated the ability of Sgg to stimulate CRC cell proliferation. Thus, we designate this locus as the Sggpathogenicity-associated region (SPAR). More importantly, we found that SPAR is important for Sgg pathogenicity in vivo. In a gut colonization model, mice exposed to the SPAR deletion mutant showed significantly reduced Sgg load in the colonic tissues and fecal materials, suggesting that SPAR contributes to the colonization capacity of Sgg. In a mouse model of CRC, deletion of SPAR abolished the ability of Sgg to promote the development of colon tumors growth. Taken together, these results highlight SPAR as a critical pathogenicity determinant of Sgg.
Aftiphilin regulation of myosin light chain kinase activity promotes actin dynamics and intestinal epithelial barrier function
Authors: Man Law Ivy Ka , Fang Kai , Pothoulakis Charalabos , Rankin Carl Robert 
Published: March 2022

AbstractThe expression levels of aftiphilin (AFTPH) are significantly lower in inflamed colonic tissues from patients with ulcerative colitis (UC) and mice with experimental colitis. During colonic inflammation, the selective permeability of the colonic epithelium is compromised largely due to dysregulation of proteins associated with either the tight junction (TJ) complex and actin-myosin contraction rings. Here, we hypothesized that inflammation-associated reduction in AFTPH levels might cause an increase in the selective permeability of the colonic epithelium. In this study, we measured the transepithelial electric resistance (TEER), sodium (Na+) ion flux and dextran permeability in polarized colonic epithelial cells after manipulation of AFTPH. Silencing of AFTPH reduced TEER, increased Na+ ion flow and dextran permeability. Examination of mRNA and protein levels of multiple TJ proteins and Na+ ion transporters suggested that AFTPH deficiency did not significantly change expression of most of these transmembrane proteins. While the gross structure of the TJs in AFTPH gene-silenced cells appeared normal, elevated levels of junctional Occludin were observed. Most notably we observed that AFTPH co-localized with myosin light chain kinase (MLCK) and attenuated cellular MLCK activity as observed by phospho-myosin light chain 2 (pMLC2) western blots. Importantly, inhibition of MLCK activity reversed the reduction of TEER in AFTPH-deficient monolayers. Lastly, examination on transmission electron microcopy on microvilli and immunofluorescent microscopy on actin filament arrangement showed that AFTPH deficiency also affected filament arrangement in colonic epithelial cells. Taken together, these results suggest that AFTPH regulates intestinal epithelial permeability and actin polymerization in colonic epithelium through interfering MLCK/MLC interactions.
KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cells
Authors: Mashima Yu , Nohira Hayato , Sugihara Hiroki , Dynlacht Brian David , Kobayashi Tetsuo , Itoh Hiroshi 
Published: March 2022

AbstractClustering of supernumerary centrosomes, potentially leading to cell survival and chromosomal instability, is frequently observed in cancers. However, the molecular mechanisms by which centrosome clustering is controlled in cancer cells remain largely unknown. A centrosomal kinesin, KIF24, was previously shown to restrain the assembly of primary cilia in mammalian cells. Here, we revealed that KIF24 depletion suppresses multipolar spindle formation by clustering centrosomes in pancreatic ductal adenocarcinoma (PDAC) cells harboring supernumerary centrosomes. KIF24 depletion also induced hyperproliferation and improved the mitotic progression in PDAC cells. On the other hand, disruption of primary cilia failed to affect the proliferation and spindle formation in KIF24-depleted cells. These results represent a novel role of KIF24 in suppressing centrosome clustering independent of primary ciliation in centrosome-amplified PDAC cells.
The X-ray crystallography phase problem solved thanks to AlphaFold and RoseTTAFold models: a case-study report
Authors: Barbarin-Bocahu Irène , Graille Marc 
Published: March 2022

The breakthrough recently made in protein structure prediction by deep-learning programs such as AlphaFold and RoseTTAFold will certainly revolutionize biology over the coming decades. The scientific community is only starting to appreciate the various applications, benefits and limitations of these protein models. Yet, after the first thrills due to this revolution, it is important to evaluate the impact of the proposed models and their overall quality to avoid the misinterpretation or overinterpretation of these models by biologists. One of the first applications of these models is in solving the `phase problem' encountered in X-ray crystallography in calculating electron-density maps from diffraction data. Indeed, the most frequently used technique to derive electron-density maps is molecular replacement. As this technique relies on knowledge of the structure of a protein that shares strong structural similarity with the studied protein, the availability of high-accuracy models is then definitely critical for successful structure solution. After the collection of a 2.45 Å resolution data set, we struggled for two years in trying to solve the crystal structure of a protein involved in the nonsense-mediated mRNA decay pathway, an mRNA quality-control pathway dedicated to the elimination of eukaryotic mRNAs harboring premature stop codons. We used different methods (isomorphous replacement, anomalous diffraction and molecular replacement) to determine this structure, but all failed until we straightforwardly succeeded thanks to both AlphaFold and RoseTTAFold models. Here, we describe how these new models helped us to solve this structure and conclude that in our case the AlphaFold model largely outcompetes the other models. We also discuss the importance of search-model generation for successful molecular replacement.
Influence of nano-alumina sealing treatment on corrosion resistance of Fe-based amorphous coatings
Authors: Wei Xinlong , Fu Erguang , Ban Aolin , Zhu Wy , Wu Dl , Li N. , Zhang C. 
Published: March 2022

Purpose
The purpose of this paper is to investigate the effect of nano-alumina sealant sealing treatment on corrosion behavior of the Fe-based amorphous coatings deposited on 304 stainless steel plates by atmospheric plasma spraying (APS) with different hydrogen flow rates.

Design/methodology/approach
The surface morphology and microstructure of the unsealed and sealed coatings were characterized by scanning electron microscopy and X-ray diffraction. The corrosion resistance of the coatings was investigated by potentiodynamic polarization test and electrochemical impedance spectroscopy experiment in 3.5 Wt.% NaCl solution.

Findings
Results show that a few microcracks and pores exist in the as-sprayed Fe-based amorphous coatings. The pores on the surface of the coatings after sealing treatment have been filled with nano-alumina sealant, which can effectively prevent corrosive medium from entering into coatings. Electrochemical tests results show that the corrosion resistance of the coatings before sealing treatment decreases with the increase of hydrogen flow rate and is significantly improved by sealing treatment.

Originality/value
The effect of nano-alumina sealant sealing treatment on corrosion resistance of APS-sprayed Fe-based amorphous coatings is revealed. The corrosion resistance of the as-sprayed Fe-based amorphous coating can be significantly improved by nano-alumina sealant sealing treatment because of the blocking effect of nano-alumina sealant on corrosive medium, which confirms that the application of nano-alumina sealant sealing treatment is of a practical option to improve corrosion resistance of as-sprayed thermal sprayed coatings.
Multilevel and multisite leadership development from a leadership-as-practice perspective: an integrative literature review
Authors: Lehtonen Salla , Seeck Hannele 
Published: March 2022

Purpose
This paper reviews what has been written on leadership development from the leadership-as-practice (L-A-P) perspective, which views leadership as emerging in everyday activities and interactions of a collective in a specific context. This paper aims to deepen the theoretical understanding of how leadership can be learned and developed from the L-A-P perspective.

Design/methodology/approach
An integrative literature review was undertaken to review and synthesise what has been written on the topic in journal articles and scholarly books.

Findings
The importance of the context and the practices that are embedded in it is the most central aspect affecting leadership development from the L-A-P perspective. This places workplace leadership development centre stage, but several papers also showed that leadership programmes have an important role. Not only collective capacity building is emphasised in the papers, but the importance of individual-level leader development is also recognised.

Originality/value
The contribution of this study is twofold: First, it brings the currently fractured information on L-A-P development together to enhance theory building by providing a synthesis of the literature. Second, a conceptual framework is constructed to show how the L-A-P perspective on leadership development can take both leadership development at the collective and individual levels into account, as well as the learning that takes place either inside or outside the workplace. This study’s results and framework show that the development has its own specific purpose and suggested methods in both levels, in both learning sites.
Service modularity in e-learning programs: an analysis from the perceived usefulness perspective
Authors: Sorkun Metehan Feridun , Yurt Oznur , Hsuan Juliana 
Published: March 2022

PurposeThis study investigates the effects of service modularity on the perceived usefulness (PU) of e-learning programs through the perceived ease of use (PEoU) and service customization.Design/methodology/approachStructural equation modeling was used to test four hypotheses with survey data from 517 undergraduates in Turkey.FindingsResults show that service modularity affects the PU of e-learning programs through the PEoU. Service customization negatively moderates the effect of service modularity on the PEoU, but positively moderates the effect of the PEoU on the PU of e-learning programs.Practical implicationsThis study offers insights that support the decisions of policymakers and higher education institutions on how to design appealing e-learning programs cost-effectively.Social implicationsThis study reveals the determinants of the PU of e-learning, which could support the democratization of access to higher education in emerging countries where barriers to higher education are relatively greater than in developed countries.Originality/valueThe concept of service modularity is explored in the e-learning context from the students' perspective. This study shows that the standardized interfaces across course modules increase the PU of e-learning programs by improving the ease of use. It also shows, interestingly, that service customization, enabled by modularity, is not always appreciated by service consumers, because of the potential extra effort demanded in communicating their unique needs to service providers.
Oral health in prison: an integrative review
Authors: Evensen Kjersti Berge , Bull Vibeke Hervik 
Published: March 2022

Purpose
Prisoners’ oral health is an often overseen, yet important aspect of the general health of prisoners. To develop effective measures to improve the situation, it is important to get an overview of existing research. The purpose of this study is to examine and analyse existing research on oral health of prisoners and provide a thorough understanding of prisoners’ oral health and related factors.

Design/methodology/approach
Due to relatively few scientific papers on prisoners’ oral health, an integrative review was chosen. Integrative reviews allow for the inclusion of diverse methodologies. A literature search was conducted, followed by an assessment of the quality of the studies by using the Mixed Methods Appraisal Tool. Thematic analysis was used to analyse the studies.

Findings
The few studies addressing European prisoners’ oral health in the last 21 years differ in design and methodology. The results from this review indicate that prisoners’ oral health is a complex phenomenon and should be understood from both individual and organisational perspectives. More research is needed to bridge the gap in the literature on prisoners’ oral health.

Originality/value
To the best of the authors’ knowledge, this is the first literature review on European prisoners’ oral health.
The antecedent of event attachment influence on tourism sustainability: the case of Jember Fashion Carnaval, Indonesia
Authors: Kusumawati Andriani , Pangestuti Edriana , Supriono Supriono 
Published: March 2022

Purpose
This study aims to highlight the influence of community attachment and community involvement on perceived value and social impact with event attachment, emphasizing the mediating effect on tourism sustainability.

Design/methodology/approach
A quantitative approach is used in this study to explain the causality relationship based on statistical analysis. The total sample collected in distributing questionnaires to the communities involved in the Jember Fashion Carnaval is 221 from various types of communities. Construct this study’s validity, reliability and hypothesis testing using partial least squares structural equation modeling (PLS-SEM) to produce SEM.

Findings
The results of this study that show positive and significance are community attachment and community involvement on perceived value, community attachment and community involvement on perceived social impact, perceived social impact on event attachment and event attachment on tourism sustainability. Meanwhile, the effect of the perceived value relationship on event attachment is not significant. This study also presents the mediating role, which has also been described.

Research limitations/implications
The study was only conducted on the most significant event in Jember City, Indonesia, and therefore, the results cannot be generalized for other cities worldwide.

Practical implications
The proposed study model suggests the need to review the current condition of event community members, especially regarding the attachment and closeness they feel to maintain the event’s sustainability.

Social implications
Achieving sustainable tourism in different types of event tourism (cultural, exhibition and culinary) requires specific development models concerning existing community and environmental conditions.

Originality/value
This study emphasizes the sustainability of tourism, as explained in the event tourism literature that the role and involvement of the community greatly contribute to the success of an event’s sustainability. Therefore, the community must also feel the social impact as an essential aspect of increasing the sustainability of participation aimed at improving quality, image and value.
Female labour force participation rate and economic growth in sub-Saharan Africa: “a liability or an asset”
Authors: Thaddeus Kesuh Jude , Bih Dimna , Nebong Njimukala Moses , Ngong Chi Aloysius , Mongo Eric Achiri , Akume Akume Daniel , Onwumere Josaphat Uchechukwu Joe 
Published: March 2022

PurposeThis paper aimed examining the contribution of female labour force participation rate on economic growth in the sub-Saharan Africa during the period of 1991–2019.Design/methodology/approachThe study employed a sample of 42 sub-Sahara African countries using annual data from the World Bank development indicators. The long-run causal effect of female labour force and economic growth was analysed using the Autoregressive Distributed Lag model and Granger causality test for causality and direction since the variables did not have the same order of integration.FindingsThe estimated results indicate that a long-run causal relationship exists between female labour force and economic growth in sub-Sahara Africa and the direction of causality is unidirectional running from economic growth to female labour force. The results also showed that female labour force participation rate negatively and significantly contributes to economic growth (GDP) is sub-Saharan Africa in the long run with an insignificantly negative contribution in the short run hence a liability.Research limitations/implicationsThe author recommends the promotion of women's economic empowerment to encourage female labour force participation to increase economic growth in the entire sub-Saharan region.Practical implicationsThis paper adds to existing literature by using more comprehensive and up to econometric analysis and variables. This paper also makes further recommendation on how female labour force participation can boost economic growth in sub-Saharan Africa (SSA).Originality/valueThis paper adds to existing literature by using more comprehensive and up to econometric analysis and variables. This paper also makes further recommendation on how female labour force participation can boost economic growth in SSA.