bluuebunny/crossref_metadata_2025_split huggingface dataset:
How can we solve the social crisis after COVID-19 and before robotization of workplace in the EU and national level – basic income or general minimum wage?
Authors: Mélypataki Gábor 
Published: August 2022

The changes of the labour market have strong characteristics compared to the earlier eras. These changes are primarily the changes happening due to the process of digitalization coming to the foreground which will significantly transform the structure of the labour market2. The time shift between the terminating workplaces and the new ones resulting from the application of new technologies and the retraining of the employees will result in an intermediate time interval. The maximal use of this time interval will be typical mainly for the low-educated manpower. However, based on the principle of national social care, these people cannot be left without provision. This problem has become really urgent because of the COVID-19 epidemic reaching Europe on the spring of 2020. More countries have introduced basic income or a similar kind of social benefit. The reason for this step is the significant employment crisis which is the consequence of the lock-down that has been applied in certain countries in order to stop the spread of the virus. But basic income is only one element of the system, so the issue should be examined in terms of minimum wage and minimum income.
Pengaruh Fasilitas dan Pelayanan Terhadap Kepuasan Pelanggan Indomaret Panasera di Batam
Authors: Sonya Debora Permata , Suhardi Suhardi 
Published: August 2022

Penelitian ini dilakukan untuk menganalisis pengaruh Fasilitas dan Pelayanan terhadap Kepuasan pelangan di Indomaret Panasera. Metode yang digunakan untuk pengambilam sampel dalam penelitian ini adalah accidental sampling. Sampel dalam penelitian ini adalah pelanggan Indomaret Panasera. Teknik pengumpulan data dengan menggunakan teknik kuesioner. Dan untuk mengeahui apakah ada permasalahan Pengaruh Fasilitas dan Pelayanan terhadap Kepuasan Pelangan pada Indomaret Panasera, Bagaimana pengaruh Fasilitas terhadap Kepuasan Pelangan Indomaret Panasera, Bagaimana pengaruh Pelayanan terhadap Kepuasan Pelanggan Indomaret Panasera dan Bagaimana pengaruh Fasilitas dan Pelayanan terhadap Kepuasan Pelangan Indomaret Panasera metode yang digunakan adalah analisis Regrensi Linear Berganda, Kofisie determinasi, Uji T dan Uji F Maka Hasih ini menunjukan pengaruh signifikan Fasilitas 0,763 < 0,05, Pelayanan 0,000 > 0,05 terhadap Kepuasan Pelayan 0,000 > 0,05
CircGSAP regulates the cell cycle of pulmonary microvascular endothelial cells via the miR-942-5p sponge in pulmonary hypertension
Authors: Sun Yuanyuan , Wu Wenhui , Zhao Qinhua , Jiang Rong , Li Jinling , Wang Lan , Xia Shijin , Liu Mingjie , Gong Sugang , Liu Jinming , Yuan Ping 
Published: August 2022

Background We recently demonstrated that circGSAP was diminished in lung tissues from patients with pulmonary arterial hypertension and in hypoxia-induced pulmonary microvascular endothelial cells (PMECs). However, the underlying role of circGSAP in PMECs remains unknown. The study aimed to investigate the contribution of circGSAP to proliferation, apoptosis and cell cycle of PMECs in hypoxic environment and explore the mechanism.Methods The expression of circGSAP was quantified by real-time PCR or immunofluorescence in human lung tissue and PMECs. CircGSAP plasmid, circGSAP small interfering RNA (siRNA), miRNA inhibitor and target gene siRNA were synthesized to verify the role of circGSAP on regulating the proliferation, apoptosis, and cell cycle of PMECs.Results CircGSAP levels were decreased in lungs and plasma of patients with pulmonary hypertension second to chronic obstructive pulmonary disease (COPD-PH) and were associated with poor outcomes of COPD-PH patients. Upregulation of circGSAP inhibited proliferation, apoptosis resistance and G1/S transition of PMECs. Dual luciferase reporter assays showed that circGSAP acted as a competitive endogenous RNA regulating miR-942-5p, and identified SMAD4 as a target gene of miR-942-5p, Then, we verified the functions of miR-942-5p and SMAD4 in PMECs. In addition, the effect of circGSAP siRNA on PMECs was mitigated by transfection of miR-942-5p inhibitor, and the effect of miR-942-5p inhibitor on PMECs was inhibited by SMAD4 siRNA.Conclusion Our findings demonstrated that diminished circGSAP accelerated cell cycle to facilitate cell proliferation and apoptosis resistance through competitively binding miR-942-5p to modulate SMAD4 expressions in hypoxia-induced PMECs, indicating potential therapeutic strategies for PH.
The effect of different poly fibers separator-modified materials on blocking polysulfides for high performance Li-S batteries
Authors: Meng Ling , Sun Zhaoxia , Sun Guanghang , Zhang Xiting , Dan Meng , Long Jin , Hu Jian 
Published: August 2022

Herein, we reported that KOH impregnation can generate a large number of porous structures with fruitful nitrogen self-doped groups during the carbonized process for poly (p-phenylene terephthalamide) fiber and poly (p-phenylene benzobisoxazole) fiber (denoted as PPTA and PBO, respectively). The intrinsical insulation, volume change, and shuttle effect of polysulfides then can be more significantly improved for the PBO-coated separator than the PPTA case. The discharge capacity primary achieves 1,322 mA h/g, which retains 827 mA h/g even after 200 cycles at 0.2 C for the cell with PBO-coated separator. The reversible specific discharge capacity maintains 841 mA h/g with a Coulomb efficiency of 99.7% at 5 C. The nitrogen self-doped nanocarbon particles are etched by KOH with the simple one-step preparation, which has promising application as Li-S battery cathode.
Saliva microbiome changes in thyroid cancer and thyroid nodules patients
Authors: Jiao Junjun , Zheng Youli , Zhang Qingyu , Xia Degeng , Zhang Li , Ma Ning 
Published: August 2022

ObjectiveThyroid disease has been reported to associate with gut microbiota, but the effects of thyroid cancer and thyroid nodules on the oral microbiota are still largely unknown. This study aimed to identify the variation in salivary microbiota and their potential association with thyroid cancer and thyroid nodules.MethodsWe used 16S rRNA high-throughput sequencing to examine the salivary microbiota of thyroid cancer patients (n = 14), thyroid nodules patients (n = 9), and healthy controls (n = 15).ResultsThe alpha-diversity indices Chao1 and ACE were found to be relatively higher in patients with thyroid cancer and thyroid nodules compared to healthy controls. The beta diversity in both the thyroid cancer and thyroid nodules groups was divergent from the healthy control group. The genera Alloprevotella, Anaeroglobus, Acinetobacter, unclassified Bacteroidales, and unclassified Cyanobacteriales were significantly enriched in the thyroid cancer group compared with the healthy control group. In contrast, the microbiome of the healthy controls was mainly composed of the genera Haemophilus, Lautropia, Allorhizobium Neorhizobium Pararhizobium Rhizobium, Escherichia Shigella, and unclassified Rhodobacteraceae. The thyroid nodules group was dominated by genre uncultured Candidatus Saccharibacteria bacterium, unclassified Clostridiales bacterium feline oral taxon 148, Treponema, unclassified Prevotellaceae, Mobiluncus, and Acholeplasma. In contrast, the genera unclassified Rhodobacteraceae and Aggregatibacter dominated the healthy control group. The study also found that clinical indicators were correlated with the saliva microbiome.ConclusionThe salivary microbiota variation may be connected with thyroid cancer and thyroid nodules.
Meaningful engagement: A crossfunctional framework for digital therapeutics
Authors: Strauss Gabriel , Flannery Jessica E. , Vierra Elise , Koepsell Xin , Berglund Emily , Miller Ian , Lake Jessica I. 
Published: August 2022

Digital mental health interventions, or digital therapeutics, have the potential to transform the field of mental health. They provide the opportunity for increased accessibility, reduced stigma, and daily integration with patient's lives. However, as the burgeoning field continues to expand, there is a growing concern regarding the level and type of engagement users have with these technologies. Unlike many traditional technology products that have optimized their user experience to maximize the amount of time users spend within the product, such engagement within a digital therapeutic is not sufficient if users are not experiencing an improvement in clinical outcomes. In fact, a primary challenge within digital therapeutics is user engagement. Digital therapeutics are only effective if users sufficiently engage with them and, we argue, only if users meaningfully engage with the product. Therefore, we propose a 4-step framework to assess meaningful engagement within digital therapeutics: (1) Define the measure of value (2) Operationalize meaningful engagement for your digital therapeutic (3) Implement solutions to increase meaningful engagement (4) Iteratively evaluate the solution's impact on meaningful engagement and clinical outcomes. We provide recommendations to the common challenges associated with each step. We specifically emphasize a cross-functional approach to assessing meaningful engagement and use an adolescent-focused example throughout to further highlight developmental considerations one should consider depending on their target users.
Comparison of the characteristics of six salt crusts
Authors: Xu Boshi , Li Chengzhi , Li Zhaoti 
Published: August 2022

Salt crust is a soil layer formed by the cementation of soluble salt on the surface or inside of soil with soil particles. The salt crust can alter the soil’s ability to resist wind erosion. To explore the characteristics of different types of salt crust, six kinds of salt (CaCl2, KCl, Na2SO4, NaCl, Na2CO3, and NaHCO3) were selected and six salt contents (0.2, 0.5, 1, 2, 3, and 4%) were set up to conduct the experiment of a sandy soil crust. The experiment compares the characteristics of crust appearance, compressive strength, and toughness of six selected kinds of salt crust. The results showed that Na2SO4 formed the subflorescence, while CaCl2, KCl, NaCl, Na2CO3, and NaHCO3 formed the efflorescences. The compressive strength and toughness of Na2SO4 salt crust initially increased and then decreased as the salt content increased, and both the strength and toughness reached the maximum when the salt content was 3%. At first, the compressive strength of NaCl crusts increased and then stabilized as a result of the increase in salt content. The toughness of the NaCl salt crust experienced an initial increase and then a decrease, reaching the maximum when the salt content was 3%. The compressive strength and toughness of the salt crusts of CaCl2 and KCl increased as the salt content increased. There was a slight increase in the compressive strength of the crusts formed by Na2CO3 and NaHCO3, with hardly any noticeable change even as the salt content increased, and the maximum compressive strength was only 0.03 and 0.40 MPa, respectively. The crusts of Na2CO3 and NaHCO3 were susceptible to wind erosion, while the crusts of KCl, CaCl2, NaCl, and Na2SO4, having high compressive strength and crust toughness, were capable of resisting wind erosion.
Single-Cell RNA Sequencing Reveals Smooth Muscle Cells Heterogeneity in Experimental Aortic Dissection
Authors: Xu Cheng , Liu Xiaowei , Fang Xiaoxin , Yu Lei , Lau Hui Chong , Li Danlei , Liu Xiaoman , Li Haili , Ren Justin , Xu Baohui , Jiang Jianjun , Tang Lijiang , Chen Xiaofeng 
Published: August 2022

Purpose: This study aims to illustrate the cellular landscape in the aorta of experimental aortic dissection (AD) and elaborate on the smooth muscle cells (SMCs) heterogeneity and functions among various cell types.Methods: Male Apolipoprotein deficient (ApoE−/−) mice at 28 weeks of age were infused with Ang II (2,500 ng/kg/min) to induce AD. Aortas from euthanized mice were harvested after 7 days for 10×Genomics single-cell RNA sequencing (scRNA-seq), followed by the identification of cell types and differentially expressed genes (DEGs). Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was conducted.Results: AD was successfully induced in ApoE−/− mice. scRNA-seq identified 15 cell clusters and nine cell types, including non-immune cells (endothelials, fibroblasts, and SMCs) and immune cells (B cells, natural killer T cell, macrophages, dendritic cells, neutrophils, and mast cells). The relative numbers of SMCs were remarkably changed, and seven core DEGs (ACTA2,IL6,CTGF,BGN,ITGA8,THBS1, and CDH5) were identified in SMCs. Moreover, we found SMCs can differentiate into 8 different subtypes through single-cell trajectory analysis.Conclusion: scRNA-seq technology can successfully identify unique cell composition in experimental AD. To our knowledge, this is the first study that provided the complete cellular landscape in AD tissues from mice, seven core DEGs and eight subtypes of SMCs were identified, and the SMCs have evolution from matrix type to inflammatory type.
Construction and validation of an angiogenesis-related gene expression signature associated with clinical outcome and tumor immune microenvironment in glioma
Authors: Hu Tianhao , Wang Yutao , Wang Xiaoliang , Wang Run , Song Yifu , Zhang Li , Han Sheng 
Published: August 2022

Background: Glioma is the most prevalent malignant intracranial tumor. Many studies have shown that angiogenesis plays a crucial role in glioma tumorigenesis, metastasis, and prognosis. In this study, we conducted a comprehensive analysis of angiogenesis-related genes (ARGs) in glioma.Methods: RNA-sequencing data of glioma patients were obtained from TCGA and CGGA databases. Via consensus clustering analysis, ARGs in the sequencing data were distinctly classified into two subgroups. We performed univariate Cox regression analysis to determine prognostic differentially expressed ARGs and least absolute shrinkage and selection operator Cox regression to construct a 14-ARG risk signature. The CIBERSORT algorithm was used to explore immune cell infiltration, and the ESTIMATE algorithm was applied to calculate immune and stromal scores.Results: We found that the 14-ARG signature reflected the infiltration characteristics of different immune cells in the tumor immune microenvironment. Additionally, total tumor mutational burden increased significantly in the high-risk group. We combined the 14-ARG signature with patient clinicopathological data to construct a nomogram for predicting 1-, 3-, and 5-year overall survival with good accuracy. The predictive value of the prognostic model was verified in the CGGA cohort. SPP1 was a potential biomarker of glioma risk and was involved in the proliferation, invasion, and angiogenesis of glioma cells.Conclusion: In conclusion, we established and validated a novel ARG risk signature that independently predicted the clinical outcomes of glioma patients and was associated with the tumor immune microenvironment.
Case report: Prenatal diagnosis of Kagami–Ogata syndrome in a Chinese family
Authors: Hu Junjie , Zhang Ying , Yang Yanmei , Wang Liya , Sun Yixi , Dong Minyue 
Published: August 2022

The aim of this work was to explore the genetic cause of the proband (Ⅲ2) presenting with polyhydramnios and gastroschisis. Copy number variation sequencing (CNV-seq), methylation-specific multiplex PCR (MS-PCR), and methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) were used to characterize the genetic etiology. CNV-seq revealed a deletion of 732.26 kb at 14q32.2q32.31 in the proband (Ⅲ2) and its mother (Ⅱ2). MS-PCR showed the maternal allele was missing in the proband, while paternal allele was missing in its mother. MS-MLPA showed deletion of the DLK1, MEG3, MIR380, and RTL1 genes of both the proband and its mother. MEG3 imprinting gene methylation increased in the proband, while decreased in its mother. It was indicated that a maternally transmitted deletion was responsible for Kagami–Ogata syndrome in the proband (Ⅲ2), and the de novo paternal deletion resulted in Temple syndrome in the mother (Ⅱ2). Prenatal diagnosis was provided at 17+3 weeks of pregnancy on the mother’s fourth pregnancy (Ⅲ4). Fortunately, the karyotype and single-nucleotide polymorphism array (SNP array) results were normal. The current investigation provided the detection methods for imprinted gene diseases, expanded the phenotype spectrum of the disease, and obtained the insight into the diagnosis, prenatal diagnosis, and genetic counseling of the disease.
Cross-sectional and longitudinal associations of serum Cysteine-rich 61 with severity and prognosis among community-acquired pneumonia patients in China
Authors: Yao Meng-Xing , Cheng Jia-Yi , Liu Ying , Sun Jing , Hua Dong-Xu , He Qi-Yuan , Liu Hong-Yan , Fu Lin , Zhao Hui 
Published: August 2022

BackgroundCysteine-rich 61 (CYR61) is implicated in many pulmonary diseases. However, the relationship between CYR61 and community-acquired pneumonia (CAP) patients was unknown. This research aimed to estimate the correlations of serum CYR61 with severity and prognosis in CAP patients through a prospective cohort study.MethodsAll 541 CAP patients were enrolled in this study. Fasting venous blood was collected. Clinical characteristics and demographic information were obtained. CYR61 and inflammatory cytokines were detected in serum using ELISA.ResultsSerum CYR61 was gradually increased in parallel with severity scores in CAP patients. Correlative analysis indicated that serum CYR61 was strongly associated with many clinical parameters in CAP patients. Moreover, mixed logistic and linear regression models found that there were positive correlations between serum CYR61 and CAP severity scores after adjusted for age, BMI, and respiratory rate. Stratified analyses suggested that age affected the associations between serum CYR61 and severity scores. On admission, higher serum CYR61 levels elevated the risks of mechanical ventilation, vasoactive agent, ICU admission, death, and longer hospital stays during hospitalization. Moreover, serum CYR61 in combination with severity scores upregulated the predictive capacities for severity and death than single serum CYR61 or severity scores in CAP patients.ConclusionThere are significantly positive dose-response associations of serum CYR61 on admission with the severity and adverse prognostic outcomes, demonstrating that CYR61 is involved in the pathophysiology of CAP. Serum CYR61 may be used as a potential biomarker for the diagnosis and prognosis in CAP patients.
Molecular identification of T-box transcription factor 6 and prognostic assessment in patients with congenital scoliosis: A single-center study
Authors: Zhang Wenyan , Yao Ziming , Guo Ruolan , Li Haichong , Zhao Shuang , Li Wei , Zhang Xuejun , Hao Chanjuan 
Published: August 2022

BackgroundCongenital scoliosis (CS) is characterized by vertebral malformations. The precise etiology of CS is not fully defined. A compound inheritance of TBX6 was identified in 10% of patients with CS in Han Chinese and formed a distinguishable subtype named TBX6-associated congenital scoliosis (TACS).MethodsTo investigate the variants and risk haplotype of TBX6, we recruited 121 patients with CS at Beijing Children’s Hospital. We collected the clinical characteristics and surgical treatment options and followed their postoperative prognoses.ResultsEight patients (6.6%) were molecularly diagnosed with TACS and carried the previously defined pathogenic TBX6 compound heterozygous variants. All the eight patients with TACS had the typical TACS clinical feature of hemivertebrae in the lower part of the spine. These patients received posterior hemivertebra resection combined with segmental fusion. Follow-ups revealed satisfactory correction without postoperative complications.ConclusionWe observed a 6.6% prevalence of TACS in our CS cohort. Follow-ups further highlighted that surgical treatment of hemivertebra resection combined with segmental fusion performed well with prognosis for patients with TACS. This could provide valuable information for CS individuals with compound heterozygosity in TBX6.
Discriminating different materials by means of vibrations
Authors: Lisini Baldi Tommaso , Marullo Sara , D’Aurizio Nicole , Prattichizzo Domenico 
Published: August 2022

Material characterization and discrimination is of interest for multiple applications, ranging from mechanical engineering to medical and industrial sectors. Despite the need for automated systems, the majority of the existing approaches necessitate expensive and bulky hardware that cannot be used outside ad-hoc laboratories. In this work, we propose a novel technique for discriminating between different materials and detecting intra-material variations using active stimulation through vibration and machine learning techniques. A voice-coil actuator and a tri-axial accelerometer are used for generating and sampling mechanical vibration propagated through the materials. Results of the present analysis confirm the effectiveness of the proposed approach. Processing a mechanical vibration signal that propagates through a material by means of a neural network is a viable means for material classification. This holds not only for distinguishing materials having gross differences, but also for detecting whether a material underwent some slight changes in its structure. In addition, mechanical vibrations at 500 Hz demonstrated an ability to provide a compact and meaningful representation of the data, sufficient to categorize 8 different materials, and to distinguish reference materials from other defective materials, with an average accuracy greater than 90%.