bluuebunny/crossref_metadata_2025_split huggingface dataset:
An explicit time-domain method for non-stationary random analysis of nonlinear frame structures with plastic hinges
Authors: Huang Huan , Li Yuyu , Li Wenxiong , Tang Guihe , Lv Yanmei 
Published: September 2022

AbstractIn this study, a novel approach for random vibration analysis of nonlinear frame structures under seismic random excitations is developed. The explicit time-domain method is improved in this approach by integrating the plastic hinge model, which can simulate the nonlinear behaviors caused by material property changes. Specifically, the hysteretic system’s equation of motion is constructed using auxiliary differential equations that govern the plastic rotational displacements and their corresponding hysteretic displacements. Additionally, by introducing the concept of equivalent excitations, an explicit iteration scheme for solving the equation of the hysteretic system is developed, in which the auxiliary differential equations are solved under the assumption that the plastic rotational velocity changes linearly with time between two adjacent time instants. Finally, by combining the Monte Carlo simulation method and the proposed explicit time-domain method, the non-stationary random responses of nonlinear frame structures can be obtained. As illustrated by numerical examples, the proposed method achieves satisfactory solution accuracy and efficiency when applied to nonlinear frame structures with plastic hinges. Moreover, the proposed iterative method resolves equations involving displacements describing the frame’s global state, plastic rotational displacements, and corresponding hysteretic parameters, introducing a novel concept for solving problems with nonlinear coupled variables of multiple types.
Substitute parameters of exercise-induced pulmonary hypertension and usefulness of low workload exercise stress echocardiography in mitral regurgitation
Authors: Amano Masashi , Nakagawa Shoko , Moriuchi Kenji , Nishimura Hitomi , Tamai Yurie , Mizumoto Ayaka , Yanagi Yoshiki , Yonezawa Rika , Demura Yutaka , Jo Yoshito , Irie Yuki , Okada Atsushi , Kitai Takeshi , Amaki Makoto , Kanzaki Hideaki , Kusano Kengo , Noguchi Teruo , Nishimura Kunihiro , Izumi Chisato 
Published: September 2022

AbstractIn asymptomatic patients with mitral regurgitation (MR), data of exercise-induced pulmonary hypertension (EIPH) are limited, and feasibility of evaluating EIPH is not high. We aimed to investigate prognostic impact of EIPH and its substitute parameters. Exercise stress echocardiography (ESE) were performed in 123 consecutive patients with moderate to severe degenerative MR. The endpoint was a composite of death, hospitalization for heart failure, and worsening of symptoms. EIPH [tricuspid regurgitation peak gradient (TRPG) at peak workload ≥ 50 mmHg] was shown in 57 patients (46%). TRPG at low workload was independently associated with TRPG at peak workload (β = 0.67, p < 0.001). Early surgical intervention (within 6 months after ESE) was performed in 65 patients. Of the remaining 58 patients with the watchful waiting strategy, the event free survival was lower in patients with EIPH than in patients without EIPH (48.1 vs. 97.0% at 1-year, p < 0.001). TRPG at low workload ≥ 35.0 mmHg as well as EIPH were associated with poor prognosis in patients with the watchful waiting strategy. In conclusion, the importance of ESE and evaluating EIPH in patients with MR was re-acknowledged. TRPG at peak workload can be predicted by TRPG at low workload, and TRPG at low workload may be useful in real-world clinical settings.
The interacting effects of irrigation, sowing date and nitrogen on water status, protein and yield in pea (Pisum sativum L.)
Authors: Ghodsi Abolfazl , Honar Tooraj , Heidari Bahram , Salarpour Mahdiyeh , Etemadi Mohammad 
Published: September 2022

AbstractManagement for agronomic practices might improves growth and grain yield in pea. The main objective of this experiment was to assess the interacting effects of different irrigation regimes, sowing date and nitrogen fertilizer treatments on pea traits. We evaluated three irrigation regimes (50, 75, and 100% of the plant irrigation requirement), two sowing dates (February and March), and nitrogen [application of nitroregn (N1) and without nitrogen as control (N0)] in 2019 and 2020 under field conditions. Chlorphyll content, leaf area index, leaf water potential, grain yield and water productivity were higher in the late sowing (March) than in early sowing (February) treatment. Percentage of vegetation cover in late sowing (60%) was significantly higher than in early sowing (52.7%) treatment. Grain yield in 75% water requirement treatment was not significantly different from yield in full irrigation treatment. Application of nitrogen fertilizer significantly reduced grain yield, grain protein and seeds per pod whilst increased chlorophyll content only. The 100% irrigation requirement treatment showed higher evaporation form the soil in N0 than in 50% and 75% irrigation treatments in late sown pea. Leaf evapotranspiration (ET) was lower in 50% water requirement irrigation regime than in the other irrigation treatments. Water use efficiency (WUE) which was higher in the late than early sowing treatment did not differ between 50% and full irrigation treatments in N0. In conclusion, the results of the current study suggested that application of nitrogen fertilizer did not benefit pea growth and that management of irrigation regime in late sowing might improve grain yield in pea and save irrigation water in regions with limited water availability.
Malate synthase contributes to the survival of Salmonella Typhimurium against nutrient and oxidative stress conditions
Authors: Sarkhel Ratanti , Apoorva Shekhar , Priyadarsini Swagatika , Sridhar Hari Balaji , Bhure Sanjeev Kumar , Mahawar Manish 
Published: September 2022

AbstractTo survive and replicate in the host, S. Typhimurium have evolved several metabolic pathways. The glyoxylate shunt is one such pathway that can utilize acetate for the synthesis of glucose and other biomolecules. This pathway is a bypass of the TCA cycle in which CO2 generating steps are omitted. Two enzymes involved in the glyoxylate cycle are isocitrate lyase (ICL) and malate synthase (MS). We determined the contribution of MS in the survival of S. Typhimurium under carbon limiting and oxidative stress conditions. The ms gene deletion strain (∆ms strain) grew normally in LB media but failed to grow in M9 minimal media supplemented with acetate as a sole carbon source. However, the ∆ms strain showed hypersensitivity (p < 0.05) to hypochlorite. Further, ∆ms strain has been significantly more susceptible to neutrophils. Interestingly, several folds induction of ms gene was observed following incubation of S. Typhimurium with neutrophils. Further, ∆ms strain showed defective colonization in poultry spleen and liver. In short, our data demonstrate that the MS contributes to the virulence of S. Typhimurium by aiding its survival under carbon starvation and oxidative stress conditions.
Lateral shoots removal has little effect on berry growth of grapevine (Vitis vinifera L.) ‘Riesling’ in cool climate
Authors: Ye Qiuhong , Wang Hua , Li Hua 
Published: September 2022

AbstractBunch compactness is an important trait that affects the sanitary status and quality of wine grapes. Many studies have demonstrated that canopy managements, such as leaf removal, shoot trimming, and postponed first shoot topping, can effectively reduce compactness. However, few studies have determined the effects of canopy management measures on bunch compactness. Shoot wrapping has been previously shown to elongate the rachis length and reduce bunch compactness. Here, we tested whether the presence of laterals affects cluster growth inVitis viniferaL. ‘Riesling’ through a field experiment with four treatments over two consecutive seasons: shoot wrapping with laterals, shoot wrapping without laterals, hedging with laterals, and hedging without laterals. Laterals were removed weekly. Lateral removal had little effect on cluster compactness; the effect was shown temporarily and not consistent the growing seasons. The effect of laterals on cluster compactness and rachis length slightly varied with years. The short-term and variable effect of laterals may be explained by the fact that they experienced little competition with clusters.
Confrontation of AlphaFold models with experimental structures enlightens conformational dynamics supporting CYP102A1 functions
Authors: Urban Philippe , Pompon Denis 
Published: September 2022

AbstractConformational dynamics plays a critical role for the function of multidomain electron transfer complexes. While crystallographic or NMR approaches allow detailed insight into structures, lower resolution methods like cryo-electron microscopy can provide more information on dynamics. In silico structure modelling using AlphaFold was recently successfully extended to the prediction of protein complexes but its capability to address large conformational changes involved in catalysis remained obscure. We used bacterial CYP102A1 monooxygenase homodimer as a test case to design a competitive modelling approach (CMA) for assessing alternate conformations of multi-domain complexes. Predictions were confronted with published crystallographic and cryo-EM data, evidencing consistencies but also permitting some reinterpretation of experimental data. Structural determinants stabilising the new type of domain connectivity evidenced in this bacterial self-sufficient monooxygenase were analysed by CMA and used for in silico retro-engineering applied to its eukaryotic bi-component counterparts.
Hydrophobicity is a key determinant in the activity of arginine-rich cell penetrating peptides
Authors: Allen Jason , Pellois Jean-Philippe 
Published: September 2022

AbstractTo deliver useful biological payloads into the cytosolic space of cells, cell-penetrating peptides have to cross biological membranes. The molecular features that control or enhance this activity remain unclear. Herein, a dimeric template of the arginine-rich HIV TAT CPP was used to establish the effect of incorporating groups and residues of various chemical structures and properties. A positive correlation is established between the relative hydrophobicity of these additional moieties and the ability of the CPP conjugates to deliver a peptidic probe into live cells. CPP conjugates with low hydrophobicity lead to no detectable delivery activity, while CPPs containing groups of increasing hydrophobicity achieve intracellular delivery at low micromolar concentrations. Notably, the chemical structures of the hydrophobic groups do not appear to play a role in overall cell penetration activity. The cell penetration activity detected is consistent with endosomal escape. Leakage assays with lipid bilayer of endosomal membrane composition also establish a positive correlation between hydrophobicity and membrane permeation. Overall, these results indicate that the presence of a relatively hydrophobic moiety, regardless of structure, is required in a CPP structure to enhance its cell penetration. It also indicates that simple modifications, including fluorophores used for cell imaging or small payloads, modulate the activity of CPPs and that a given CPP-conjugate may be unique in its membrane permeation properties.
Epidemiologic Changes of Facial Bone Fracture before and after Coronavirus Disease 2019: A Level 1 Trauma Center in Korea
Authors: Kim Jeong Ho , Yang Chae Eun , Kim Sug Won , Kim Jiye 
Published: September 2022

Abstract
Background The coronavirus disease 2019 (COVID-19) outbreak has had a major impact worldwide. Several countries have implemented restrictions on social interaction (“social distancing”). Several studies have reported that the epidemiology of trauma patients, such as those with facial bone fractures, has changed after COVID-19 pandemic. This study aimed to further explore these specific changes.
Methods This was a retrospective study of patients who presented to a single institution with facial bone fractures between January 1, 2016, and December 31, 2020. Baseline patient demographics, clinical information, type of fracture, etiology, and operative management were compared before and after COVID-19.
Results Of all cases, 3,409 occurred before COVID-19, and 602 occurred after COVID-19. Since the outbreak of COVID-19, the number of patients with facial fractures has not decreased significantly. A significant increase was noted in fractures that occurred outdoors (p < 0.001). However, a decrease was observed in operative management between the groups (p < 0.001). There was no significant difference in the proportion of assault, fall-down, industrial accident, or roll-down. In contrast, the proportion of traffic accidents and slip-down categories increased significantly (p < 0.05). Moreover, a significant decrease was found in the proportion of the sports category (p = 0.001)
Conclusions It was confirmed through this study that COVID-19 pandemic also affected epidemiology of facial fractures. Focusing on these changes, it is necessary to develop safety measures to reduce facial fractures.
Selective Neurectomy for Treatment of Post-Facial Paralysis Synkinesis: A Systematic Review
Authors: Shikara Meryam , Bridgham Kelly , Ludeman Emilie , Vakharia Kalpesh , Justicz Natalie 
Published: September 2022

Abstract
Background Synkinesis is commonly encountered after flaccid facial paralysis and can have a detrimental impact on a patient's life. First-line treatment of synkinesis is chemodenervation with botulinum toxin (Botox) and neuromuscular retraining. Surgical options include selective myectomy, selective neurectomy (SN), cross-facial nerve grafting, nerve substitution, and free gracilis muscle transfer. Data on surgical management of synkinesis using SN is limited.
Evidence Review PubMed, Embase, Cochrane CENTRAL, Cochrane Neuromuscular Register, Clinicaltrials.gov, and World Health Organization International Clinical Trials Registry Platform were searched using a comprehensive keyword strategy in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. All English-only texts published in the past 20 years were included. Two independent investigators reviewed 906 abstracts and 7 studies met inclusion criteria. Demographics, etiology of paralysis, time to surgery, and primary outcomes studied were collected.
Findings A total of 250 patients were included across 7 studies. In 6 out of 7 studies, Botox was used prior to surgical intervention. Two studies showed significant reduction in Botox dosage postoperatively, while one study showed no difference. Other primary outcomes included the House–Brackmann Score, palpebral fissure width, electronic clinician-graded facial function scale (eFACE) score, Facial Clinimetric Evaluation (FaCE) scale, and Synkinesis Assessment Questionnaire (SAQ). Three studies showed significant improvement in the eFACE score, two studies showed significant improvement in the FaCE scale, while one study showed improvement in quality of life measured by the SAQ.
Conclusion SN can be considered as an adjunct to other management options including neuromuscular retraining, Botox, selective myectomy, and reanimation procedures. While there is great heterogeneity of study design in the studies included, many cohorts showed improvement in facial symmetry, facial function, and quality of life. There remains a great gap in knowledge in this subject matter and a need for large well-designed prospective studies comparing this technique to other management options.
Pedicle First Anterior Approach to Harvest Anterolateral Thigh Flap—Review of 304 Cases
Authors: Kalra G.S. , Gupta Samarth , Kalra Sushrut 
Published: September 2022

AbstractBackground Although considered as a workhorse flap, the anterolateral thigh (ALT) flap has a steep learning curve that makes it difficult for microsurgeons to perform it early in their practice. In over 85% of patients, the perforator takes an intramuscular course making it difficult for beginners to safely secure the perforator dissection. In this technique, the pedicle is dissected first, utilizing the proximal incision by palpating the groove in between vastus lateralis and rectus femoris on the anterior aspect and extending the incision from 2 to 3 cm distal to the inguinal ligament to the flap markings caudally. Exposing the pedicle first makes it easier to proceed toward the skin perforator due to its easy identification and larger size at its origin.Patients and Methods This retrospective study was conducted from 2005 to 2020 in which 304 ALT flaps were performed by the pedicle first technique. Flap harvest time, incidence of injury to the skin perforator during harvest, flap re-exploration rates, and postoperative complications including incidence of flap necrosis, infection, and bleeding were the parameters that were measured.Results This study included a total of 304 patients of which 220 were male (72.3%). The average flap harvest time was 26 ± 3.2 minutes. Adverse events included perforator injury (n = 1), flap re-exploration (n = 15), and complete flap loss (n = 8). The last eight patients were reconstructed secondarily with ALT flap from the opposite side and free latissimus dorsi flap (n = 2).Conclusion The pedicle first technique makes ALT flap harvest easy, safe, and faster for plastic surgeons. The chances of injury to the skin perforator are markedly less thereby reducing postoperative complications.
Biomechanical Analysis of a New Eight-Strand Suture for Flexor Tendon Repair
Authors: Moriya Koji , Maki Yutaka , Koda Hisao , Tsubokawa Naoto 
Published: September 2022

Abstract
Background The placement of multistrand sutures during flexor tendon repair requires complicated surgical skills; such suturing is difficult. We developed a new, simpler eight-strand suture, which we term the Yoshizu cross-lock. This reduces the numbers of suture passages through the tendons, as well as the numbers of knots.
Methods Fourteen porcine flexor tendons were transected and repaired using the Yoshizu cross-lock system; no peripheral sutures were placed. Our system is a modification of the published, exposed cross-lock repair method that employs a 4–0 monofilament nylon two-strand line and two needles. The repaired tendons were subjected to linear, noncyclic load-to-failure tensile testing. The initial gap, the 2-mm gap force, and the ultimate strength were measured.
Results The initial gap force was 12.6 ± 5.6 Newtons (N), the 2-mm gap force was 33.9 ± 10.9 N, and the ultimate strength was 70.1 ± 17.0 N. All tendons subjected to Yoshizu cross-lock repair failed due to suture rupture rather than pullout.
Conclusions Our biomechanical study revealed that Yoshizu cross-lock repair had sufficient tensile strength but was associated with wide variation in the 2-mm gap load (standard deviation = 10.9 N). This study is clinically relevant, showing that the Yoshizu cross-lock repair combined with peripheral suturing may allow a repaired flexor tendon to withstand the stresses encountered during early active mobilization. This simple eight-strand technique will be particularly useful to surgeons who commonly employ the cross-lock stitch for primary flexor tendon repair following early mobilization.
Variability in practices for drinking water vaccination of meat chickens against infectious laryngotracheitis
Authors: Groves Peter J. , Assen Awol M. , Etherington Ashley , Stillman Mark , Alfirevich Sheridan , Gerber Priscilla F. , Langfield Alex-Kate , Walkden-Brown Stephen W. 
Published: September 2022

Context Drinking water vaccination of young meat chickens with Infectious Laryngotracheitis (ILT) vaccine is problematic. Vaccine failure and adverse vaccine reactions are frequently reported. Variations in the technique of applying ILT vaccines by this mass vaccination method need to be understood to contribute to improving the success of vaccination. Aims This study aimed to examine variations in the techniques of application of Infectious Laryngotracheitis vaccines via drinking water for young meat chickens. Methods Drinking water vaccination techniques were observed and recorded across 52 broiler flocks during ILT outbreaks in three geographic areas of Australia. Descriptive statistics for all variables were computed and variations between integrator company procedures were statistically compared. Key results Despite rigorous standard operating procedures, wide variations were observed in time of water deprivation prior to vaccination (3–15 min), time drinking water was stabilised prior to addition of vaccine and the type of stabiliser product used, time to activate the flock following filling of the water lines with vaccine (10–127 min), time for the vaccine to be consumed (36–226 min) and the volume of drinking water per bird used to provide the vaccine (11–48 mL/bird). Conclusions Variation in vaccination technique can affect the success of drinking water vaccination against ILT in young meat chickens. Implications Understanding the importance of the variable factors in vaccine application method can improve the success of water vaccination against ILT.
Fabrication of extreme wettability surface for controllable droplet manipulation over a wide temperature range
Authors: Shu Chengsong , Su Qitong , Li Minghao , Wang Zhenbin , Yin Shaohui , Huang Shuai 
Published: September 2022

Abstract
Droplet controllable manipulation over a wide temperature range has promising applications in microelectronic heat dissipation, inkjet printing, and high temperature microfluidic system. However, the fabrication of a platform for controllable droplet manipulation using the methods commonly used in industry remains a tremendously challenge. The popular method of controlling droplets is highly dependent on external energy input and has relatively poor controllability in terms of droplet motion behaviors and manipulation environment, such as distance, velocity, direction and a wide temperature range. Here, we report a facile and industrially applicable method for preparing Al superhydrophobic (S-phobic) surfaces, which enables controlled droplet bouncing, evaporation, and transport over a wide temperature range. Systematic mechanistic studies are also investigated. Extreme wettability surfaces were prepared on Al substrate by a composite process of electrochemical mask etching and micro-milling. To investigate the evaporation process and thermal coupling characteristics, controlled evaporation and controlled bouncing of droplet in a wide temperature range were conducted. Based on the evaporation regulation and bouncing mechanism of droplets on an extreme wettability surface, by using Laplace pressure gradients and temperature gradients, we realized controlled transport of droplets with confluence, split-flow, and gravity-resistant transport over a wide temperature range, offering a potential platform for a series of applications, such as new drug candidates and water collection.