bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Dietary, Serum and Urine Analysis of Boron and Micronutrients in Postmenopausal Women
Authors: GULBAHAR Aysegul , ÇAKAL, PH.D. Gaye , DİNÇER CENGİZ Sevim , ÇAĞLAR Gamze Sinem 
Published: October 2022

Objective: Boron is a nutritionally important trace element that interacts with other micronutrients. Boron plays a critical role in bone mineralization and metabolism. In the present study, the association between boron and micronutrients related to bone metabolism was analysed in postmenopausal women.
Methods: In a prospective cohort study in 40 postmenopausal women 24-hour urine and blood samples were collected for sodium, phosphorus, calcium, magnesium, and boron. Daily food consumption, bone mineral density, and Fracture risk assessment tool scores were recorded.
Results: The mean age was 53.2 ± 5.9 years. Dietary habits revealed insufficient dietary fiber and excessive dietary sodium. The serum and urine boron levels were 26.80 μg/L and 21.22 μg/day, respectively. Urine boron levels were lower in the osteoporosis group (p = 0.66). A negative correlation between urine Na and boron was detected (p < 0.001). Urinary Na and Ca are negatively correlated with Fracture risk assessment tool scores (p = 0.010, p = 0.019, respectively).
Conclusion: The low urinary boron levels in our participants might be due to increased Na excretion due to excessive consumption of Na. Therefore, consulting postmenopausal women about their dietary habits is of concern. Further understanding of the role of boron in bone metabolism will help to accomplish new treatment strategies for osteoporosis and standardization of boron supplementation.
Evaluation of D-Dimer and Neutrophil/Lymphocyte Ratios of COVID-19 Patients Whom Applied to Karapınar State Hospital
Authors: KADER Saadet , AKDAĞ Turan , SARIYILDIZ Levent , ÖZEL Zeynep 
Published: October 2022

Objective: The epidemic which caused by the SARS-CoV-2 virus were defined as COVID-19) and declared as a global pandemic by the World Health Organization (WHO) on March, 2020. Nowadays, many biochemical parameters related to the diagnosis and prognosis of COVID-19 are being investigated. Therefore, we aimed to evaluate D-dimer and neutrophil/lymphocyte ratios (NLR) of COVID-19 patients whom applied to Karapınar State Hospital.
Methods: Patients which consisted of 2523, whom diagnosed with COVID-19 between 11 March 2019 and 29 July 2021 at Karapınar State Hospital were included in the study. Age, gender and social history of the patients were recorded. From the results, the relationships between D-dimer and hemogram were evaluated.
Results: There was a high correlation between the variables HCT and HGB, PLT and PCT, NEUT# and WBC, and MCH and MCV (r=0.981, r=0.944, r=0.923, r=0.925). In addition, there was a high correlation between RBC and HCT and between RBC and HGB variables (r=0.852, r=0.795). There was a moderate correlation between WBC and MO#, MCHC and MCH, PDW and MPV (r=0.562, r=0.639, r=0.64). All the relationships between these variables were positive, and the value of the correlated parameter increases linearly by unit. Also, the highest positive relationships were between HCT and HGB, PLT and PCT, NEUT# and WBC, MCH and MCV. Moreover, D-dimer and NLR were not correlated (r= – 0.015, p=0.49).
Conclusion: In the study, no correlation was observed between D-dimer and neutrophil/lymphocyte ratios of COVID-19 patients. So, more comprehensive and further studies are needed to clarify these results.
Does the Presence of Metabolic Syndrome Alter Serum Uric Acid Concentrations, Pain, and Well-Being in Patient with Chronic Musculoskeletal Pain?
Authors: TOPRAK ÇELENAY Şeyda , ÖZER KAYA Derya , ŞAŞ Senem 
Published: October 2022

Objective: To compare serum uric acid concentrations, pain and well-being in patients having chronic musculoskeletal pain with and without MetS, and investigate cut-off values.
Methods: Patients having chronic musculoskeletal pain with (MetS group, n=48) and without MetS (control group, n=52) were included. The serum uric acid concentration, pain intensity, body composition, physical activity level, quality of life, and psychological status were evaluated by a uric acid blood test, Visual Analogue Scale, Bio-impedance Analyzer, International Physical Activity Questionnaire-7 (IPAQ-7), Nottingham Health Profile, and Hospital Anxiety and Depression Scale, respectively.
Results: Uric acid level, fat mass, waist/hip ratio were found higher in the MetS group in comparison to the control group (P
Reconstruction and optimization of a Pseudomonas putida-Escherichia coli microbial consortium for mcl-PHA production from lignocellulosic biomass
Authors: Qin Ruolin , Zhu Yinzhuang , Ai Mingmei , Jia Xiaoqiang 
Published: October 2022

The demand for non-petroleum-based, especially biodegradable plastics has been on the rise in the last decades. Medium-chain-length polyhydroxyalkanoate (mcl-PHA) is a biopolymer composed of 6–14 carbon atoms produced from renewable feedstocks and has become the focus of research. In recent years, researchers aimed to overcome the disadvantages of single strains, and artificial microbial consortia have been developed into efficient platforms. In this work, we reconstructed the previously developed microbial consortium composed of engineered Pseudomonas putida KT∆ABZF (p2-a-J) and Escherichia coli ∆4D (ACP-SCLAC). The maximum titer of mcl-PHA reached 3.98 g/L using 10 g/L glucose, 5 g/L octanoic acid as substrates by the engineered P. putida KT∆ABZF (p2-a-J). On the other hand, the maximum synthesis capacity of the engineered E. coli ∆4D (ACP-SCLAC) was enhanced to 3.38 g/L acetic acid and 0.67 g/L free fatty acids (FFAs) using 10 g/L xylose as substrate. Based on the concept of “nutrient supply-detoxification,” the engineered E. coli ∆4D (ACP-SCLAC) provided nutrient for the engineered P. putida KT∆ABZF (p2-a-J) and it acted to detoxify the substrates. Through this functional division and rational design of the metabolic pathways, the engineered P. putida-E. coli microbial consortium could produce 1.30 g/L of mcl-PHA from 10 g/L glucose and xylose. Finally, the consortium produced 1.02 g/L of mcl-PHA using lignocellulosic hydrolysate containing 10.50 g/L glucose and 10.21 g/L xylose as the substrate. The consortium developed in this study has good potential for mcl-PHA production and provides a valuable reference for the production of high-value biological products using inexpensive carbon sources.
Fabrication of a lead-free ternary ceramic system for high energy storage applications in dielectric capacitors
Authors: Khan Azam , Gul Noor Shad , Luo Mao , Wu Jianbo , Khan Shahan Zeb , Manan Abdul , Wang Xiu-Jian , Khan Taj Malook 
Published: October 2022

The importance of electroceramics is well-recognized in applications of high energy storage density of dielectric ceramic capacitors. Despite the excellent properties, lead-free alternatives are highly desirous owing to their environmental friendliness for energy storage applications. Herein, we provide a facile synthesis of lead-free ferroelectric ceramic perovskite material demonstrating enhanced energy storage density. The ceramic material with a series of composition (1-z) (0.94Na0.5Bi0.5TiO3-0.06BaTiO3)-zNd0.33NbO3, denoted as NBT-BT-zNN, where, z = 0.00, 0.02, 0.04, 0.06, and 0.08 are synthesized by the conventional solid-state mix oxide route. Microphases, microstructures, and energy storage characteristics of the as-synthesized ceramic compositions were determined by advanced ceramic techniques. Powder X-ray diffraction analysis reveals pure single perovskite phases for z = 0 and 0.02, and secondary phases of Bi2Ti2O7 appeared for z = 0.04 and 0.08. Furthermore, scanning electron microscopy analysis demonstrates packed-shaped microstructures with a reduced grain size for these ceramic compositions. The coercive field (Ec) and remnant polarization (Pr) deduced from polarization vs. electric field hysteresis loops determined using an LCR meter demonstrate decreasing trends with the increasing z content for each composition. Consequently, the maximum energy storage density of 3.2 J/cm3, the recoverable stored energy of 2.01 J/cm3, and the efficiency of 62.5% were obtained for the z content of 2 mol% at an applied electric field of 250 kV/cm. This work demonstrates important development in ceramic perovskite for high power energy storage density and efficiency in dielectric capacitors in high-temperature environments. The aforementioned method makes it feasible to modify a binary ceramic composition into a ternary system with highly enhanced energy storage characteristics by incorporating rare earth metals with transition metal oxides in appropriate proportions.
Inspiratory muscle training in patients with heart failure: A systematic review and meta-analysis
Authors: Li Hui , Tao Lingling , Huang Yuewi , Li Ziyang , Zhao Jianrong 
Published: October 2022

ObjectiveTo explore the effect of inspiratory muscle training (IMT) on patients with heart failure and further explore the impact of IMT on patients with heart failure with preserved ejection fraction.MethodsPubMed, EMBASE, Cochrane Library, CNKI, Wanfang and VIP databases were systematically searched. Randomized controlled trials of inspiratory muscle training in patients with heart failure were included. Revman 5.3 software was used to calculate the weighted mean difference (MD) of the combined effect size. The effects of IMT on the maximum oxygen uptake (peakVO2), maximum inspiratory pressure (PImax), ventilation efficiency (VE/VCO2), six-minute walking distance (6MWD), forced expiratory volume (FEV1), forced vital capacity (FVC) and quality of life in patients with heart failure were compared and analyzed.ResultsAfter systematic retrieval and screening, 17 studies were included in this study, and the quality of the included studies was good. The results showed that IMT could increase peakVO2 (MD 2.53; 95% CI 1. 54, 3. 51; P &lt; 0.0001) and PImax (MD 17.25; 95% CI 13. 75, 20. 75; P &lt; 0.00001); improve the VE/VCO2 (MD −4.22; 95% CI −6.78, −1.66; P = 0.001) and significantly improve the quality of life in patients with heart failure (MD −13.34; 95% CI −20.42, −6.26; P = 0.0002). However, the effect of IMT on 6MWD in patients with heart failure was not statistically significant (MD 74.45; 95% CI −12.88,161.79; P = 0.09), and the effect on lung function (FEV1 and FVC) was also not statistically significant (P = 0.08; P = 0.86). IMT had a more significant positive effect on peakVO2 (MD 2.98; 95% CI 1.63, 4.34; P &lt; 0.0001) and quality of life (MD −14.52; 95% CI −18.53, −10.52; P &lt; 0.00001) in patients with heart failure with preserved ejection fraction. Descriptive analysis suggested that IMT may positively affect dyspnoea in patients with heart failure. In addition, the choice of evaluation scale may affect the evaluation results of quality of life and dyspnoea.ConclusionIMT has a significant positive effect on respiratory status in patients with heart failure, but different dyspnoea and quality of life evaluation scales can affect the final evaluation results.
Vacuolization in embryos on days 3 and 4 of in vitro development: Association with stimulation protocols, embryo development, chromosomal status, pregnancy and neonatal outcomes
Authors: Chen Longbin , Zhang Shuoping , Gu Yifan , Peng Yangqin , Huang Zenghui , Gong Fei , Lin Ge 
Published: October 2022

Study questionIs vacuolization in embryos on Days 3 and 4 associated with parent-related factors, stimulation protocols, embryo development, embryo ploidy, pregnancy and neonatal outcomes?Study design, size, durationThis is a retrospective cohort study that comprised 5,703 embryos from 611 patients who underwent preimplantation genetic testing and time-lapse monitoring of their embryos from August 2017 to September 2021.Main resultsEmbryo vacuolization on Days 3 and 4 is associated with the LH level on the day of the hCG trigger and the number of retrieved oocytes. Compared to vacuole-negative embryos, the rates of blastocyst formation and good-blastocyst formation was significantly lower in vacuole-positive embryos. We observed no significant difference in the rates of euploidy, implantation, ongoing pregnancy, and live birth between vacuole-positive and vacuole-negative embryos. In vacuole-positive embryos, the embryos of which the vacuole-positive blastomeres were involved in embryo compaction exhibited significantly higher mosaicism rate compared with those of which the vacuole-positive blastomeres were not involved in embryo compaction.ConclusionVacuolization in embryos on Days 3 and 4 is associated with reduced blastocyst formation rate and high-quality blastocyst rate. Blastocysts had a low mosaicism rate if the vacuole-containing cells were rejected in compaction process, which supports the hypothesis that exclusion of abnormal blastomeres from compaction is a self-correction mechanism.
Yin Huo Tang, a traditional Chinese herbal formula, relives ovariectomy and empty bottle stimulation-induced menopause-like symptoms in mice
Authors: Ye Yang , Zhang Bo , Li Yan , Xu Hong-Dan , Liu Xiu-Min , Huang Shu-Ming , Wang Rui , Li Dong 
Published: October 2022

BackgroundYin Huo Tang (YHT), a traditional Chinese herbal formula, is effectively used for the clinical treatment of menopause-like symptoms in China. This study aimed to investigate its efficacy on menopause-like symptoms in mice using behavioral tests and histopathological assessment, and to determine its possible mechanism of action based on network pharmacology.MethodsLiquid chromatography-mass spectrometry (LC-MS) technology was used to identify the potential active ingredients of YHT. In mice, menopause-like symptoms were induced by combination of bilateral ovariectomy and empty bottle stimulation. The mice were then treated with the YHT aqueous extract for three weeks. Behavior, sleep state, body weight, organ index, and histomorphology were analyzed separately. Additionally, network pharmacology and molecular docking were used to predict the mechanisms underlying the action of YHT. Finally, serum estradiol was quantified to preliminarily verify the results of network pharmacology.ResultsYHT not only improved the behavior of mice (attack and explore behavior reduced; modify behavior increased) but also ameliorated the sleep state (sleep time increased and incubation time reduced). YHT reduced body weight, increased uterine weight, and improved the histomorphology of some organs. Network pharmacology and molecular docking analyses revealed that the estrogen signaling pathway might play a key role in attenuating menopause-like symptoms. Furthermore, YHT treatment reversed the reduction in serum estradiol levels.ConclusionsYHT alleviates menopause-like symptoms in a mouse model, providing a rationale for using it as a potential therapeutic strategy.
Transcriptional data analysis reveals the association between infantile hemangiomas and venous malformations
Authors: Huang Biao , Zhang Ping , Zhong Yuan-Yuan , Wang Kuan , Chen Xiao-Ming , Yu Dao-Jiang 
Published: October 2022

Background: Infantile hemangiomas (IH) and venous malformations (VM) are the most common types of vascular abnormalities that seriously affect the health of children. Although there is evidence that these two diseases share some common genetic changes, the underlying mechanisms need to be further studied.Methods: The microarray datasets of IH (GSE127487) and VM (GSE7190) were downloaded from GEO database. Extensive bioinformatics methods were used to investigate the common differentially expressed genes (DEGs) of IH and VM, and to estimate their Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Trough the constructing of protein-protein interaction (PPI) network, gene models and hub genes were obtained by using Cytoscape and STRING. Finally, we analyzed the co-expression and the TF-mRNA-microRNA regulatory network of hub genes.Results: A total of 144 common DEGs were identified between IH and VM. Functional analysis indicated their important role in cell growth, regulation of vasculature development and regulation of angiogenesis. Five hub genes (CTNNB1, IL6, CD34, IGF2, MAPK11) and two microRNA (has-miR-141-3p, has-miR-150-5p) were significantly differentially expressed between IH and normal control (p &lt; 0.05).Conclusion: In conclusion, our study investigated the common DEGs and molecular mechanism in IH and VM. Identified hub genes and signaling pathways can regulate both diseases simultaneously. This study provides insight into the crosstalk of IH and VM and obtains several biomarkers relevant to the diagnosis and pathophysiology of vascular abnormalities.
Characteristics of clinical trials for non-small cell lung cancer therapeutic vaccines registered on ClinicalTrials.gov
Authors: Gu Wenyue , Xu Yangjie , Chen Xiaohong , Jiang Hao 
Published: October 2022

BackgroundEven after complete surgical treatment or chemotherapy, Non-Small Cell Lung Cancer (NSCLC) patients are also at substantial risk for recurrence and spread trend. Therapeutic cancer vaccination could increase the anti-tumor immune response and prevent tumor relapse. This study aimed to assess the characteristics of NSCLC therapeutic vaccines registered on ClinicalTrials.gov.MethodsWe conducted a cross-sectional, descriptive study of clinical trials for Non-Small Cell Lung Cancer Therapeutic Vaccines Registered on ClinicalTrials.gov (https://clinicaltrials.gov/) through March 17, 2022.ResultsThis study encompassed 117 registered trials included for data analysis. The number of trials was significantly correlated with a beginning year (r = 0.504, P &lt; 0.010). Of these trials, 45.30% were completed, 12.82% were terminated, and 8.55% were withdrawn. More than half of trials (52.99%) were funded by industry, and more than half of trials (52.14%) were located in economically developed North America. Regarding study designs of these trials, 27.35% were randomized, 52.14% were single group assignment, 83.76% were without masking, 35.90% were phase 1, and more than half of the trials (56.41%) recruited less than 50 participants. The highest proportion of vaccine types was protein/peptide vaccines (41.88%). Regarding TNM staging, the highest proportion of the trials is stage III-IV (26.50%).ConclusionThe number of clinical trials about the cancer therapeutic vaccines was sustained an increase in recent years. The main characteristic of clinical trials for NSCLC therapeutic vaccines is lack of randomized control, lack of mask, and recruiting less than 50 participants. In recent years, the protein/peptide vaccines for NSCLC active immunotherapy have been well studied.
Ultrasonic inspection of near surface defects with additive manufactured metasurface lens
Authors: Zhu Qi , Zhang Yuanjun , Zhang Haiyan , Memoli Gianluca 
Published: October 2022

Acoustic metasurfaces have been increasingly explored in the past 10 years because of their compact structure and wave manipulation capabilities. In this work, we explore the feasibility of using an acoustic metasurface lens (MSL) for the characterization of near surface defects in NDT. First, we delimited the design space for a MSL operating at 100 kHz, in terms of spatial footprint and materials, and then realized a library of labyrinthine cells optimized for transmission. Then, we assembled the unit cells into lens and simulated its performance in a hole inspection process. The time-domain simulations predicted an increase in scattering and interface effects but, thanks to the focusing behavior, they also showed a much lower drop in the peak amplitude (20%, compared to 70% without MSL). Finally, we validated the simulations using a UTR9000-based MSL on an acrylic sample. Experiments with the MSL showed a converging energy profile, reduced to half of its width without the lens, and the presence of additional transmission peaks allowed to determine the defect diameter with excellent precision, thus circumventing near surface interference. Our study may be the first step towards flexible beam control systems based on a single transducer.
Establishment of a model for predicting delayed post-polypectomy bleeding: A real-world retrospective study
Authors: Lu Yu , Zhou Xiaoying , Chen Han , Ding Chao , Si Xinmin 
Published: October 2022

BackgroundDelayed post-polypectomy bleeding (DPPB) is the most common complication which occurs within 30 days after polypectomy, it has become rather common with the widespread of colorectal cancer screening. It is important to clarified predictors of DPPB and identify patients at high risk.Materials and methodsThis was a real-world retrospective study based on medical records from The First Affiliated Hospital of Nanjing Medical University. Cases of patients who underwent colonoscopic polypectomy between January 2016 and December 2020 were reviewed to identify risk factors of DPPB. We use the LASSO-Logistic regression analysis model to identify independent predictors and create a predictive model. The model finally got visualized by developing a nomogram.ResultsColonoscopic polypectomy was done on 16,925 patients in our study. DPPB occurred in 125 (0.74%) of these instances. In multivariate analysis, age, sex, hypertension, polyp location, polyp size, and operative modality were found to be independent risk factors and were integrated for the construction of a nomogram. The model’s C-index is 0.801 (95%CI: 0.761–0.846). We also found polyps located at the right semicolon and polyp ≥ 1 cm associated with active bleeding under the therapeutic colonoscopy.ConclusionYoung age, male, hypertension, polyp ≥ 1 cm, proximal colon location and operative modality were finally identified as significant predictors of DPPB. We developed and validated a nomogram which performs well in predicting the incidence of DPPB, the model we established can be used as a valuable screening tool to identify patients who are at high risk of bleeding.
Is there an epilepsy belt of high prevalence rate in China?
Authors: Wu Li , Ye Zeming , Li Youfu , Luo Ming , Li Shaojun , Mi Hongyan , Qiu Zhihua , Jiang Yongjun 
Published: October 2022

BackgroundEpilepsy is one of the leading neurological diseases. Our study is aimed to determine whether there is a focal region of high epilepsy prevalence in China.MethodsAll studies published between 1981 and 2020 investigating the prevalence of epilepsy in China were systematically reviewed. The geographical location, sample size, number of cases, urbanization rate, gross domestic product (GDP) per capita, percentage of &lt;15 years old, and medical insurance per capita were derived and analyzed. Criteria for a provincial region of high prevalence was defined as with higher epilepsy prevalence than the average prevalence of epilepsy in China.ResultsA total of 60 studies provided data on the prevalence of epilepsy in 29 of 33 provincial regions of China. The average prevalence in China was 1.68 per 1,000, and 12 provincial regions met our criteria for a region of high epilepsy prevalence and constitute an epilepsy belt ranging along the division between the second step and the third step of China. The prevalence in the epilepsy belt was 331.9 per 100,000 population compared with 125.3 per 100,000 in regions outside the belt (P &lt; 0.05). Surprisingly, there was no significant difference in sample size, number of cases, urbanization rate, GDP per capita, percentage of &lt;15 years old, or medical insurance per capita between the regions in and outside the epilepsy belt.ConclusionsAn epilepsy belt of high prevalence exists in 12 provincial regions locating along the division between the second step and the third step of China.