bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stress relief during annealing of railway wheel steel characterized by synchrotron X-ray micro-diffraction
Authors: Zhang Yubin , Jessop Casey , Nikas Dimitrios , Yu Tianbo , Liu Wenjun , Ahlström Johan 
Published: August 2022

AbstractRailway wheels in service experience rolling contact fatigue loading, but also need to resist frictional heating on braking, yielding temperatures up to 500 °C. The combination of mechanical and thermal loads leads to changes in the mechanical properties of the material. The focus of this study is to investigate the effect of annealing on local microstructure and residual stresses in railway wheel pearlitic steel (medium carbon steels, ~0.55 wt.% C) using synchroton X-ray Laue micro-diffraction. It is found that the local residual stress releases to a large extent after annealing at 500 °C. The stress formation and relief mechanisms and their relationship to the local microstructure are discussed.
Recovery of small orientation gradients in a recrystallized grain observed in 3D during ex-situ annealing
Authors: Hong Chuanshi , Zhang Yubin , Yu Tianbo , Liu Wenjun , Jensen Dorte Juul 
Published: August 2022

Abstract
We report the observation of a small orientation gradient (~0.5° over a few micrometers) in a recrystallized grain formed at the sample surface in pure Al and the elimination of this orientation gradient during subsequential ex-situ annealing. The observation is in 3D and done using synchrotron white-beam differential-aperture X-ray microscopy with high angular resolution of 0.01°. The elimination of the orientation gradient during subsequent annealing are analyzed and discussed.
A polarization-insensitive triple-band perfect metamaterial absorber incorporating ZnSe for terahertz sensing
Authors: Abdulkarim Yadgar I , Alkurt Fatih Özkan , Bakır Mehmet , Awl Halgurd N , Muhammadsharif Fahmi F , Karaaslan Muharrem , Appasani Bhargav , Al-Badri Khalid Saeed Lateef , Zhu Yuanyuan , Dong Jian 
Published: August 2022

Abstract
In this work, a triple-band polarization-insensitive metamaterial structure with perfect absorption is proposed by incorporating a zinc selenide (ZnSe) spacer. The structure was optimally designed by varying the type of the spacer and the unit cell dimensions. The structure was simulated using the finite integration technique, and the results showed that the proposed design achieved a near-perfect absorption of about 99%, 99%, and 100% at 22.50, 28.98, and 35.14 THz, respectively. Its absorption characteristics were insensitive to the polarization angle and a wide range of incidence angles, making it an ideal absorber. Further investigations of the electric field, magnetic field, and surface current distributions were carried out to elaborate on the absorption characteristics at various resonance frequencies. The proposed device can also be used as a sensor that can detect the depth of the surrounding analyte and its refractive index. The device could detect the depth of the analyte with a peak sensitivity of 2.76 THz μm−1 and its refractive index with a peak sensitivity of 1.55 THz RIU−1. Thus, the design could have interesting terahertz applications.
Acetyl-L-carnitine attenuates Poly I:C-induced sickness behavior in mice
Authors: Miura Suzuka , Oyanagi Eri , Watanabe Chihiro , Hamada Hiroki , Aoki Takafumi , Kremenik Michael J , Yano Hiromi 
Published: August 2022

ABSTRACT
Fatigue is accompanied by a decrease in physical activity or malaise, and might be reduced by acetyl-L-carnitine (ALC) administration. The purpose of this study was to investigate the preventive effects of ALC on Poly I:C-induced sickness behavior in mice. For the experiment, male C3H/HeN mice were used and treated with ALC for 5 days before Poly I:C administration. ALC administration attenuated the decrease in wheel behavior activity of mice at 24 h after Poly I:C administration and ALC-treated mice quickly recovered from the sickness behavior. The gene expression of brain-derived neurotrophic factor (BDNF) in the cerebrum and hippocampus, which is associated with physical activity, was higher in the ALC-treated group. Translocator protein 18kDa (TSPO), which has cytoprotective effects, was up-regulated in the cerebrum and hippocampus, suggesting that ALC suppressed the decrease in activity induced by Poly I:C treatment through enhancement of cytoprotective effects in the brain.
Stability of hen egg-white lysozyme during embryonic development
Authors: Muroi Yukiko , Aburaya Izumi , Kiyokawa Yuki , Watanabe Keiichi , Wada Koji , Abe Yoshito , Sugimoto Yasushi 
Published: August 2022

ABSTRACT
It is of interest to determine whether and how egg-white proteins are maintained in fertile eggs. We previously observed that egg-white ovalbumin attained high stability during embryogenesis. Herein, we observed that the total mass of egg white and that of its gross protein content showed a decrease according to the days of incubation. The total bacteriolytic activity also lowered, in accord with previous observations. We purified lysozyme from egg-white samples on several incubation days. These purified lysozyme proteins were observed to have enzymatic and bacteriolytic activities against Micrococcus lysodeikticus as well as growth-inhibition potency against Staphylococcus aureus. As the embryogenesis proceeded, the purified lysozyme showed changes in Km and Vmax, a small decrease in the denaturation temperature, and symptoms of an increase in surface hydrophobicity. These results indicate that the lysozyme protein maintained its enzymatic and antibacterial activities until the late period of incubation while undergoing slight conformational changes.
Blood-based transcriptomic signature panel identification for cancer diagnosis: benchmarking of feature extraction methods
Authors: Vijayan Abhishek , Fatima Shadma , Sowmya Arcot , Vafaee Fatemeh 
Published: August 2022

AbstractLiquid biopsy has shown promise for cancer diagnosis due to its minimally invasive nature and the potential for novel biomarker discovery. However, the low concentration of relevant blood-based biosources and the heterogeneity of samples (i.e. the variability of relative abundance of molecules identified), pose major challenges to biomarker discovery. Moreover, the number of molecular measurements or features (e.g. transcript read counts) per sample could be in the order of several thousand, whereas the number of samples is often substantially lower, leading to the curse of dimensionality. These challenges, among others, elucidate the importance of a robust biomarker panel identification or feature extraction step wherein relevant molecular measurements are identified prior to classification for cancer detection. In this work, we performed a benchmarking study on 12 feature extraction methods using transcriptomic profiles derived from different blood-based biosources. The methods were assessed both in terms of their predictive performance and the robustness of the biomarker panels in diagnosing cancer or stratifying cancer subtypes. While performing the comparison, the feature extraction methods are categorized into feature subset selection methods and transformation methods. A transformation feature extraction method, namely partial least square discriminant analysis, was found to perform consistently superior in terms of classification performance. As part of the benchmarking study, a generic pipeline has been created and made available as an R package to ensure reproducibility of the results and allow for easy extension of this study to other datasets (https://github.com/VafaeeLab/bloodbased-pancancer-diagnosis).
Scalable batch-correction approach for integrating large-scale single-cell transcriptomes
Authors: Shen Xilin , Shen Hongru , Wu Dan , Feng Mengyao , Hu Jiani , Liu Jilei , Yang Yichen , Yang Meng , Li Yang , Shi Lei , Chen Kexin , Li Xiangchun 
Published: August 2022

AbstractIntegration of accumulative large-scale single-cell transcriptomes requires scalable batch-correction approaches. Here we propose Fugue, a simple and efficient batch-correction method that is scalable for integrating super large-scale single-cell transcriptomes from diverse sources. The core idea of the method is to encode batch information as trainable parameters and add it to single-cell expression profile; subsequently, a contrastive learning approach is used to learn feature representation of the additive expression profile. We demonstrate the scalability of Fugue by integrating all single cells obtained from the Human Cell Atlas. We benchmark Fugue against current state-of-the-art methods and show that Fugue consistently achieves improved performance in terms of data alignment and clustering preservation. Our study will facilitate the integration of single-cell transcriptomes at increasingly large scale.
The influence of imagery vividness and internally-directed attention on the neural mechanisms underlying the encoding of visual mental images into episodic memory
Authors: Gjorgieva Eva , Geib Benjamin R , Cabeza Roberto , Woldorff Marty G 
Published: August 2022

AbstractAttention can be directed externally toward sensory information or internally toward self-generated information. Using electroencephalography (EEG), we investigated the attentional processes underlying the formation and encoding of self-generated mental images into episodic memory. Participants viewed flickering words referring to common objects and were tasked with forming visual mental images of the objects and rating their vividness. Subsequent memory for the presented object words was assessed using an old–new recognition task. Internally-directed attention during image generation was indexed as a reduction in steady-state visual evoked potentials (SSVEPs), oscillatory EEG responses at the frequency of a flickering stimulus. The results yielded 3 main findings. First, SSVEP power driven by the flickering word stimuli decreased as subjects directed attention internally to form the corresponding mental image. Second, SSVEP power returned to pre-imagery baseline more slowly for low- than high-vividness later remembered items, suggesting that longer internally-directed attention is required to generate subsequently remembered low-vividness images. Finally, the event-related-potential difference due to memory was more sustained for subsequently remembered low- versus high-vividness items, suggesting that additional conceptual processing may have been needed to remember the low-vividness visual images. Taken together, the results clarify the neural mechanisms supporting the encoding of self-generated information.
Delay-related activity in marmoset prefrontal cortex
Authors: Wong Raymond K , Selvanayagam Janahan , Johnston Kevin D , Everling Stefan 
Published: August 2022

AbstractPersistent delay-period activity in prefrontal cortex (PFC) has long been regarded as a neural signature of working memory (WM). Electrophysiological investigations in macaque PFC have provided much insight into WM mechanisms; however, a barrier to understanding is the fact that a portion of PFC lies buried within the principal sulcus in this species and is inaccessible for laminar electrophysiology or optical imaging. The relatively lissencephalic cortex of the New World common marmoset (Callithrix jacchus) circumvents such limitations. It remains unknown, however, whether marmoset PFC neurons exhibit persistent activity. Here, we addressed this gap by conducting wireless electrophysiological recordings in PFC of marmosets performing a delayed-match-to-location task on a home cage-based touchscreen system. As in macaques, marmoset PFC neurons exhibited sample-, delay-, and response-related activity that was directionally tuned and linked to correct task performance. Models constructed from population activity consistently and accurately predicted stimulus location throughout the delay period, supporting a framework of delay activity in which mnemonic representations are relatively stable in time. Taken together, our findings support the existence of common neural mechanisms underlying WM performance in PFC of macaques and marmosets and thus validate the marmoset as a suitable model animal for investigating the microcircuitry underlying WM.
Normative quantitative relaxation atlases for characterization of cortical regions using magnetic resonance fingerprinting
Authors: Choi Joon Yul , Hu Siyuan , Su Ting-Yu , Murakami Hiroatsu , Tang Yingying , Blümcke Ingmar , Najm Imad , Sakaie Ken , Jones Stephen , Griswold Mark , Wang Zhong Irene , Ma Dan 
Published: August 2022

AbstractQuantitative magnetic resonance (MR) has been used to study cyto- and myelo-architecture of the human brain non-invasively. However, analyzing brain cortex using high-resolution quantitative MR acquisition can be challenging to perform using 3T clinical scanners. MR fingerprinting (MRF) is a highly efficient and clinically feasible quantitative MR technique that simultaneously provides T1 and T2 relaxation maps. Using 3D MRF from 40 healthy subjects (mean age = 25.6 ± 4.3 years) scanned on 3T magnetic resonance imaging, we generated whole-brain gyral-based normative MR relaxation atlases and investigated cortical-region-based T1 and T2 variations. Gender and age dependency of T1 and T2 variations were additionally analyzed. The coefficient of variation of T1 and T2 for each cortical-region was 3.5% and 7.3%, respectively, supporting low variability of MRF measurements across subjects. Significant differences in T1 and T2 were identified among 34 brain regions (P &lt; 0.001), lower in the precentral, postcentral, paracentral lobule, transverse temporal, lateral occipital, and cingulate areas, which contain sensorimotor, auditory, visual, and limbic functions. Significant correlations were identified between age and T1 and T2 values. This study established whole-brain MRF T1 and T2 atlases of healthy subjects using a clinical 3T scanner, which can provide a quantitative and region-specific baseline for future brain studies and pathology detection.
Coronary physiology to guide treatment of coronary artery disease in a patient with severe aortic valve stenosis: friend or foe? A case report
Authors: Minten Lennert , McCutcheon Keir , Bennett Johan , Dubois Christophe 
Published: August 2022

Abstract

Background
Severe aortic valve stenosis (AS) is the most frequent valve pathology in the developed world requiring intervention. Due to common factors in pathogenesis, patients with AS frequently have concomitant coronary artery disease (CAD). Determining the relative contribution of each component to the disease state is not easy as there is much overlap in complaints. Moreover, severe AS interferes with the haemodynamic assessment of intermediate coronary lesions.

Case summary
In this case report we describe the presentation and management of an 84-year-old patient, with a severely degenerated aortic valve bioprosthesis and an intermediate coronary artery lesion, presenting with acute decompensated heart failure and chest pain. Initial invasive haemodynamic assessment of the coronary lesion provided challenging findings and a second catheterization and intervention was needed to free the patient from his chest pain.

Discussion
Optimal assessment and treatment of CAD before valve replacement are controversial. Aortic valve stenosis on itself can lead to subendocardial ischaemia with subsequent angina pectoris. Simultaneously, AS can significantly affect coronary haemodynamics, hereby interfering with intra-coronary haemodynamic assessment of co-existing coronary lesions. Currently used coronary physiological indices are not validated in the AS population and valve replacement has variable effects on the fractional flow reserve and commonly used resting indices, such as the resting full-cycle ratio. Further research on this topic is needed and an overview of currently running studies that will advance this field significantly is provided.
Unilateral left-sided thoracoscopic ablation of atrial fibrillation concomitant to minimally invasive bypass grafting of the left anterior descending artery
Authors: van der Heijden Claudia A J , Segers Patrique , Masud Anna , Weberndörfer Vanessa , Chaldoupi Sevasti-Marisevi , Luermans Justin G L M , Bijvoet Geertruida P , Kietselaer Bas L J H , van Kuijk Sander M J , Barenbrug Paul J C , Maessen Jos G , Bidar Elham , Maesen Bart 
Published: August 2022

Abstract

OBJECTIVES
Thoracoscopic ablation for atrial fibrillation (AF) and minimally invasive direct coronary artery bypass (MIDCAB) with robot-assisted left internal mammary artery (LIMA) harvesting may represent a safe and effective alternative to more invasive surgical approaches via sternotomy. The aim of our study was to describe the feasibility, safety and efficacy of a unilateral left-sided thoracoscopic AF ablation and concomitant MIDCAB surgery.

METHODS
Retrospective analysis of a prospectively gathered cohort was performed of all consecutive patients with AF and at least a critical left anterior descending artery (LAD) stenosis that underwent unilateral left-sided thoracoscopic AF ablation and concomitant off-pump MIDCAB surgery in the Maastricht University Medical Centre between 2017 and 2021.

RESULTS
Twenty-three patients were included [age 69 years (standard deviation = 8), paroxysmal AF 61%, left atrial volume index 42 ml/m2 (standard deviation = 11)]. Unilateral left-sided thoracoscopic isolation of the left (n = 23) and right (n = 22) pulmonary veins and box (n = 21) by radiofrequency ablation was succeeded by epicardial validation of exit- and entrance block (n = 22). All patients received robot-assisted LIMA harvesting and off-pump LIMA-LAD anastomosis through a left mini-thoracotomy. The perioperative complications consisted of one bleeding of the thoracotomy wound and one aborted myocardial infarction not requiring intervention. The mean duration of hospital stay was 6 days (standard deviation = 2). After discharge, cardiac hospital readmission occurred in 4 patients (AF n = 1; pleural- and pericardial effusion n = 2, myocardial infarction requiring the percutaneous intervention of the LIMA-LAD n = 1) within 1 year. After 12 months, 17/21 (81%) patients were in sinus rhythm when allowing anti-arrhythmic drugs. Finally, the left atrial ejection fraction improved postoperatively [26% (standard deviation = 11) to 38% (standard deviation = 7), P = 0.01].

CONCLUSIONS
In this initial feasibility and early safety study, unilateral left-sided thoracoscopic AF ablation and concomitant MIDCAB for LIMA-LAD grafting is a feasible, safe and efficacious for patients with AF and a critical LAD stenosis.
Biosynthesis of insect sex pheromone precursors via engineered β-oxidation in yeast
Authors: Petkevicius Karolis , Wenning Leonie , Kildegaard Kanchana R , Sinkwitz Christina , Smedegaard Rune , Holkenbrink Carina , Borodina Irina 
Published: August 2022

Abstract
Mating disruption with insect sex pheromones is an attractive and environmentally friendly technique for pest management. Several Lepidoptera sex pheromones have been produced in yeast, where biosynthesis could be accomplished by the expression of fatty acyl-CoA desaturases and fatty acyl-CoA reductases. In this study, we aimed to develop yeast Yarrowia lipolytica cell factories for producing Lepidoptera pheromones which biosynthesis additionally requires β-oxidation, such as (Z)-7-dodecenol (Z7-12:OH), (Z)-9-dodecenol (Z9-12:OH), and (Z)-7-tetradecenol (Z7-14:OH). We expressed fatty acyl-CoA desaturases from Drosophila melanogaster (Dmd9) or Lobesia botrana (Lbo_PPTQ) and fatty acyl-CoA reductase from Helicoverpa armigera (HarFAR) in combinations with 11 peroxisomal oxidases of different origins. Yeast cultivations were performed with supplementation of methyl myristate (14:Me). The oxidase Lbo_31670 from L. botrana provided the highest titers of (Z)-7-dodecenoate, (Z)-9-dodecenoate, and (Z)-7-tetradecenoate. However, no chain-shortened fatty alcohols were produced. The mutation of fatty acid synthase (Fas2pI1220F) to increase myristate production did not lead to targeted fatty alcohol production. The problem was solved by directing the reductase into peroxisomes, where the strain with Dmd9 produced 0.10 ± 0.02 mg/l of Z7-12:OH and 0.48 ± 0.03 mg/l of Z7-14:OH, while the strain with Lbo_PPTQ produced 0.21 ± 0.03 mg/l of Z9-12:OH and 0.40 ± 0.07 mg/l of Z7-14:OH. In summary, the engineering of β-oxidation in Y. lipolytica allowed expanding the portfolio of microbially produced insect sex pheromones.
Functional Compensation of Mouse Duplicates by their Paralogs Expressed in the Same Tissues
Authors: Luzuriaga-Neira Agusto , Subramanian Krishnamurthy , Alvarez-Ponce David 
Published: August 2022

Abstract
Analyses in a number of organisms have shown that duplicated genes are less likely to be essential than singletons. This implies that genes can often compensate for the loss of their paralogs. However, it is unclear why the loss of some duplicates can be compensated by their paralogs, whereas the loss of other duplicates cannot. Surprisingly, initial analyses in mice did not detect differences in the essentiality of duplicates and singletons. Only subsequent analyses, using larger gene knockout data sets and controlling for a number of confounding factors, did detect significant differences. Previous studies have not taken into account the tissues in which duplicates are expressed. We hypothesized that in complex organisms, in order for a gene’s loss to be compensated by one or more of its paralogs, such paralogs need to be expressed in at least the same set of tissues as the lost gene. To test our hypothesis, we classified mouse duplicates into two categories based on the expression patterns of their paralogs: “compensable duplicates” (those with paralogs expressed in all the tissues in which the gene is expressed) and “noncompensable duplicates” (those whose paralogs are not expressed in all the tissues where the gene is expressed). In agreement with our hypothesis, the essentiality of noncompensable duplicates is similar to that of singletons, whereas compensable duplicates exhibit a substantially lower essentiality. Our results imply that duplicates can often compensate for the loss of their paralogs, but only if they are expressed in the same tissues. Indeed, the compensation ability is more dependent on expression patterns than on protein sequence similarity. The existence of these two kinds of duplicates with different essentialities, which has been overlooked by prior studies, may have hindered the detection of differences between singletons and duplicates.