bluuebunny/crossref_metadata_2025_split huggingface dataset:
Melatonin enhances osteogenic differentiation of dental pulp mesenchymal stem cells by regulating MAPK pathways and promotes the efficiency of bone regeneration in calvarial bone defects
Authors: Chan Ya-Hui , Ho Kuo-Ning , Lee Yu-Chieh , Chou Meng-Jung , Lew Wei-Zhen , Huang Haw-Ming , Lai Pin-Chuang , Feng Sheng-Wei 
Published: February 2022

Abstract
Background
Mesenchymal stem cell (MSC)-based tissue engineering plays a major role in regenerative medicine. However, the efficiency of MSC transplantation and survival of engrafted stem cells remain challenging. Melatonin can regulate MSC biology. However, its function in the osteogenic differentiation of dental pulp-derived MSCs (DPSCs) remains unclear. We investigated the effects and mechanisms of melatonin on the osteogenic differentiation and bone regeneration capacities of DPSCs.

Methods
The biological effects and signaling mechanisms of melatonin with different concentrations on DPSCs were evaluated using a proliferation assay, the quantitative alkaline phosphatase (ALP) activity, Alizarin red staining, a real-time polymerase chain reaction, and a western blot in vitro cell culture model. The in vivo bone regeneration capacities were assessed among empty control, MBCP, MBCP + DPSCs, and MBCP + DPSCs + melatonin preconditioning in four-created calvarial bone defects by using micro-computed tomographic, histological, histomorphometric, and immunohistochemical analyses after 4 and 8 weeks of healing.

Results
In vitro experiments revealed that melatonin (1, 10, and 100 μM) significantly and concentration-dependently promoted proliferation, surface marker expression (CD 146), ALP activity and extracellular calcium deposition, and osteogenic gene expression of DPSCs (p < 0.05). Melatonin activated the protein expression of ALP, OCN, and RUNX-2 and inhibited COX-2/NF-κB expression. Furthermore, the phosphorylation of mitogen-activated protein kinase (MAPK) p38/ERK signaling was significantly increased in DPSCs treated with 100 μM melatonin, and their inhibitors significantly decreased osteogenic differentiation. In vivo experiments demonstrated that bone defects implanted with MBCP bone-grafting materials and melatonin-preconditioned DPSCs exhibited significantly greater bone volume fraction, trabecular bone structural modeling, new bone formation, and osteogenesis-related protein expression than the other three groups at 4 and 8 weeks postoperatively (p < 0.05).

Conclusions
These results suggest that melatonin promotes the proliferation and osteogenic differentiation of DPSCs by regulating COX-2/NF-κB and p38/ERK MAPK signaling pathways. Preconditioning DPSCs with melatonin before transplantation can efficiently enhance MSCs function and regenerative capacities.
Psychosocial rehabilitation of individuals with schizophrenia: a scoping review protocol
Authors: Oyelade Oyeyemi Olajumoke , Nkosi-Mafutha Nokuthula Gloria 
Published: February 2022

Abstract
Background
The psychosocial rehabilitation of an individual with mental illness is an evidence-based approach to reducing the burden of the illness and the associated stigma globally. Specifically, in Africa, it has promising scope for African life and the African economy. Psychosocial rehabilitation is described as a set of approaches that aim to assist an individual in achieving restoration from a state of dependency caused by schizophrenia to a state of being an independent decision-maker. However, there seems to be a dearth of literature and implementation of psychosocial rehabilitation in Africa. Therefore, it is necessary to map studies on how psychosocial rehabilitation is conducted for people living in Africa with the most chronic form of mental illness, schizophrenia.

Methods
This study will adopt the Arksey and O’Malley scoping review framework to search and compile relevant studies. This process will involve three steps: title screening, to be performed solely by the principal investigator, followed by abstract and full-text screening, to be performed independently by two reviewers (the principal investigator and co-investigator). Rayyan QCRI, a systematic reviews web app, will be used for tracking the screening records, and data charting form will be used to extract basic data of included studies. The risk of bias in the articles identified for screening will be assessed by the Mixed Method Appraisal Tool (MMAT). Finally, the content analysis of the screened studies will be performed with NVivo.

Expected outcome
This study has the likelihood of revealing a research gap in psychosocial rehabilitation approaches and methods. The review results will constitute part of the available evidence that the researchers aim to adopt in the broader part of the project, which aims to develop implementation strategies for the psychosocial rehabilitation of chronic mental illnesses, specifically schizophrenia, in Sub-Sahara Africa. The implementation process also encompasses disseminating the findings of this review to stakeholders, which will enhance their knowledge of the current state of Sub-Saharan Africa and may stimulate support for the implementation of rehabilitation strategies.
Geometric properties of the meromorphic functions class through special functions associated with a linear operator
Authors: Ghanim F. , Al-Janaby Hiba F. , Bazighifan Omar 
Published: February 2022

AbstractAccording to the theory of regular geometric functions, the relevance of geometry to analysis is a critical feature. One of the significant tools to study operators is to utilize the convolution product. The dynamic techniques of convolution have attracted numerous complex analyses in current research. In this effort, an attempt is made by utilizing the said techniques to study a new linear complex operator connecting an incomplete beta function and a Hurwitz–Lerch zeta function of certain meromorphic functions. Furthermore, we employ a method based on the first-order differential subordination to derive new and better differential complex inequalities, namely differential subordinations.
Effects of service-wide support on regularity of alcohol screening of clients in Australian Aboriginal and Torres Strait Islander Community Controlled Health Services: a cluster randomised trial
Authors: Dzidowska Monika , Raubenheimer Jacques E. , Dobbins Timothy A. , Lee K. S. Kylie , Hayman Noel , Vnuk Julia , Haber Paul , Conigrave Katherine M. 
Published: February 2022

Abstract
Background
We have previously shown that service-wide support can increase the odds of alcohol screening in any 2-month period in a cluster randomized trial of service-wide support to Aboriginal and Torres Strait Islander Community Controlled Health Services (ACCHS). Here we report an exploratory analysis on whether the resulting pattern of screening was appropriate. Aim: we assess whether that increase in screening was associated with: (i) increased first-time screening, (ii) increased annual screening, (iii) whether frequently screened clients fell into one of four risk categories as defined by national guidelines.

Methods
Setting and participants: 22 ACCHS; randomized to receive the support model in the treatment (‘early-support’) arm over 24-months or to the waitlist control arm. Intervention: eight-component support, including training, sharing of experience, audit-and-feedback and resource support. Analysis: records of clients with visits before and after start of implementation were included. Multilevel logistic modelling was used to compare (i) the odds of previously unscreened clients receiving an AUDIT-C screen, (ii) odds of clients being screened with AUDIT-C at least once annually. We describe the characteristics of a sub-cohort of clients who received four or more screens annually, including if they were in a high-risk category.

Results
Of the original trial sample, 43,054 met inclusion criteria, accounting for 81.7% of the screening events in the overall trial. The support did not significantly increase the odds of first-time screening (OR  = 1.33, 95% CI 0.81–2.18, p  = 0.25) or of annual screening (OR  = 0.99, 95% CI 0.42–2.37, p = 0.98). Screening more than once annually occurred in 6240 clients. Of the 841 clients with four or more screens annually, over 50% did not fall into a high-risk category. Females were overrepresented. More males than females fell into high-risk categories.

Conclusion
The significant increase in odds of screening observed in the main trial did not translate to significant improvement in first-time or annual screening following implementation of support. This appeared to be due to some clients being screened more frequently than annually, while more than half remained unscreened. Further strategies to improve alcohol screening should focus on appropriate screening regularity as well as overall rates, to ensure clinically useful information about alcohol consumption.
Trial Registration ACTRN12618001892202, retrospectively registered 16 November 2018 https://anzctr.org.au/Trial/Registration/TrialReview.aspx?ACTRN=12618001892202.
Metamodeling techniques for CPU-intensive simulation-based design optimization: a survey
Authors: Khatouri Hanane , Benamara Tariq , Breitkopf Piotr , Demange Jean 
Published: February 2022

AbstractIn design optimization of complex systems, the surrogate model approach relying on progressively enriched Design of Experiments (DOE) avoids efficiency problems encountered when embedding simulation codes within optimization loops. However, an efficient a priori sampling of the design space rapidly becomes costly when using High-Fidelity (HF) simulators, especially in high dimension. On the other hand, in applications such as aeronautical design, multiple simulation tools are frequently available for the same problem, generally with a degree of precision inversely proportional to the CPU cost. Thus, the concept of multi-fidelity proposes to merge different levels of fidelity within a single model with controlled variance. Based on recent Reduced-Order Modeling (ROM) techniques, an alternative approach allows to pursue the objective of mastering the simulation budget by replacing costly models with their approximate full-field counterparts, providing additional insight to scalar surrogates built directly from the Quantities of Interest (QoI). Both approaches: multi-fidelity and ROM, may be combined, allowing for additional flexibility in choosing the degree of fidelity required in different zones of the design space. This paper reviews the strategies that seek to improve surrogate-based optimization efficiency, including ROM, multi-fidelity metamodeling, and DOE enrichment strategies.
Warwick Edinburgh Mental Well-Being Scale (WEMWBS): measurement invariance across genders and item response theory examination
Authors: Marmara Joshua , Zarate Daniel , Vassallo Jeremy , Patten Rhiannon , Stavropoulos Vasileios 
Published: February 2022

Abstract
Background
The Warwick Edinburgh Mental Well-Being Scale (WEMWBS) is a measure of subjective well-being and assesses eudemonic and hedonic aspects of well-being. However, differential scoring of the WEMWBS across gender and its precision of measurement has not been examined. The present study assesses the psychometric properties of the WEMWBS using measurement invariance (MI) between males and females and item response theory (IRT) analyses.

Method
A community sample of 386 adults from the United States of America (USA), United Kingdom, Ireland, Australia, New Zealand, and Canada were assessed online (N = 394, 54.8% men, 43.1% women, Mage = 27.48, SD = 5.57).

Results
MI analyses observed invariance across males and females at the configural level and metric level but non-invariance at the scalar level. The graded response model (GRM) conducted to observe item properties indicated that all items demonstrated, although variable, sufficient discrimination capacity.

Conclusions
Gender comparisons based on WEMWBS scores should be cautiously interpreted for specific items that demonstrate different scalar scales and similar scores indicate different severity. The items showed increased reliability for latent levels of ∓ 2 SD from the mean level of SWB. The WEMWBS may also not perform well for clinically low and high levels of SWB. Including assessments for clinical cases may optimise the use of the WEMWBS.
FAM83A is a potential biomarker for breast cancer initiation
Authors: Marino Natascia , German Rana , Podicheti Ram , Rockey Pam , Sandusky George E. , Temm Constance J. , Nakshatri Harikrishna , Addison Rebekah J. , Selman Bryce , Althouse Sandra K. , Storniolo Anna Maria V. 
Published: February 2022

Abstract
Background
Family with sequence similarity 83 member A (FAM83A) presents oncogenic properties in several cancers including breast cancer. Recently, we reported FAM83A overexpression in normal breast tissues from women at high risk of breast cancer. We now hypothesize that FAM83A is a key factor in breast cancer initiation.

Methods
Immunohistochemical staining was used to evaluate FAM83A protein levels in both a normal breast tissue microarray (TMA, N = 411) and a breast tumor TMA (N = 349). EGFR staining and its correlation with FAM83A expression were also assessed. Lentivirus-mediated manipulation of FAM83A expression in primary and hTERT-immortalized breast epithelial cells was employed. Biological and molecular alterations upon FAM83A overexpression/downregulation and FAM83A’s interaction partners were investigated.

Results
TMA analysis revealed a 1.5-fold increase in FAM83A expression level in breast cancer cases as compared with normal breast tissues (p < 0.0001). FAM83A protein expression was directly correlated with EGFR level in both normal and breast cancer tissues. In in vitro assays, exogenous expression of FAM83A in either primary or immortalized breast epithelial cells promoted cell viability and proliferation. Additionally, Ingenuity Pathway Analysis (IPA) revealed that FAM83A overexpression in primary cells affected the expression of genes involved in cellular morphology and metabolism. Mass spectrometry analysis identified DDX3X and LAMB3 as potential FAM83A interaction partners in primary cells, while we detected FAM83A interaction with cytoskeleton reorganization factors, including LIMA1, MYH10, PLEC, MYL6 in the immortalized cells.

Conclusions
This study shows that FAM83A promotes metabolic activation in primary breast epithelial cells and cell proliferation in both primary and immortalized cells. These findings support its role in early breast oncogenesis.
Sentiment analysis for cruises in Saudi Arabia on social media platforms using machine learning algorithms
Authors: Al sari Bador , Alkhaldi Rawan , Alsaffar Dalia , Alkhaldi Tahani , Almaymuni Hanan , Alnaim Norah , Alghamdi Najwa , Olatunji Sunday O. 
Published: February 2022

AbstractSocial media﻿ has great importance in the community for discussing many events and sharing them with others. The primary goal of this research is to study the quality of the sentiment analysis (SA) of impressions about Saudi cruises, as a first event, by creating datasets from three selected social media platforms (Instagram, Snapchat, and Twitter). The outcome of this study will help in understanding opinions of passengers and viewers about their first Saudi cruise experiences by analyzing their feelings from social media posts. After cleaning, this experiment contains 1200 samples. The data was classified into positive or negative classes using the choice of machine learning algorithms, such as multilayer perceptron (MLP), naıve bayes (NB), random forest (RF), support vector machine (SVM), and voting. The results show the highest classification accuracy for the RF algorithm, as it achieved 100% accuracy with over-sampled data from Snapchat using both test options. The algorithms were compared among the three different datasets. All algorithms achieved a high level of accuracy. Hence, the results show that 80% of the sentiments were positive while 20% were negative.
Spatiotemporal features of pollutant loads in the Yan River Basin, a typical loess hilly and gully watershed in the Chinese Loess Plateau
Authors: Song Yanni , Wu Yiping , Sun Changshun , Zhao Fubo , Hu Jingyi , Chen Ji , Qiu Linjing , Lian Yanqing 
Published: February 2022

AbstractWater quality is the restrictive factor for both ecosystem health and social development in the Chinese Loess Plateau, a unique area with most severe soil erosion, fragile ecology, and water shortage. Understanding the characteristics of the pollutant loads is of vital importance for the sustainability of eco-environment in the Loess Plateau. This study investigated the spatiotemporal changes of chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) loads by combining the Soil and Water Assessment Tool (SWAT) and regression model Load Estimator (LOADEST) in a typical loess hilly and gully watershed—the Yan River Basin (YanRB). Results showed that the model simulations of monthly streamflow and pollutant loads were in good agreement with those derived from the in situ observations. The temporal variation analysis suggested that the pollutant loads were generally rising in the study period (2001–2018) at four of the five stations and reached the maximum in 2014, and the multi-year (i.e., 2001–2018 with 2013 being excluded due to extreme rainfall) average loads of COD, TN, and TP at the Tanjiahe station, which is close to the outlet of the basin, were 15,021 kg/day, 3835 kg/day, and 168 kg/day, respectively. The spatial distribution of the TN and TP loads along the river seemed to be quite unique because the TP level were obviously higher at the midstream (e.g., Zhujiagou and Ganguyi) than the downstream (e.g., Tanjiahe), and the TN level decreased when the river flowed from Zhujiagou to Ganguyi. Further, the seasonal analysis indicated that the nutrient loads were the highest in summer, followed by autumn, and the loads in these two seasons contributed the most of the annual pollution loads—about 76% and 84% for TN and TP, respectively, indicating the higher flow, the higher pollution load, a similar point based on the inter-annual analysis. In addition, the contribution analysis of point source and non-point source pollutions demonstrated that NPS led to most of the pollutant loads at the whole watershed—70%, 67%, and 71% of the COD, TN, and TP loads, respectively. Overall, this study provided spatiotemporal distributions of the key pollutant loads in the YanRB and can be valuable for water quality protection and pollution control in this area.
Racism, sexism and disconnection: contrasting experiences of Black women in STEM before and after transfer from community college
Authors: Allen DeeDee , Dancy Melissa , Stearns Elizabeth , Mickelson Roslyn , Bottia Martha 
Published: February 2022

AbstractBackgroundRepeated calls to diversify the population of students earning undergraduate degrees in science, technology, engineering, and mathematics (STEM) fields have noted the greater diversity of community college students and their potential to thus have an impact on the racial/ethnic composition of 4-year degree earners. In this paper, we investigate barriers and supports to Black women’s success in STEM, using longitudinal interview data with seven Black women who were enrolled at community colleges and stated an interest in majoring in STEM at 4-year institutions.ResultsOur findings highlight a contrast between community colleges and universities. At community colleges, Black women were able to form supportive relationships with professors and peers, downplayed the potential of racism and sexism to derail their STEM ambitions, and saw little to no impact of bias on their educational experiences. Those students who transferred characterized university climates very differently, as they struggled to form supportive relationships and experienced racism and sexism from professors and peers.ConclusionsWe conclude using Patricia Hill Collins’ Domains of Power framework to categorize students’ experiences, then end with recommendations for change that will result in less alienating experiences for Black women, among other minoritized students.
I will teach you here or there, I will try to teach you anywhere: perceived supports and barriers for emergency remote teaching during the COVID-19 pandemic
Authors: Donham Cristine , Barron Hillary A. , Alkhouri Jourjina Subih , Changaran Kumarath Maya , Alejandro Wesley , Menke Erik , Kranzfelder Petra 
Published: February 2022

Abstract
Background
Due to the COVID-19 pandemic, many universities moved to emergency remote teaching (ERT). This allowed institutions to continue their instruction despite not being in person. However, ERT is not without consequences. For example, students may have inadequate technological supports, such as reliable internet and computers. Students may also have poor learning environments at home and may need to find added employment to support their families. In addition, there are consequences to faculty. It has been shown that female instructors are more disproportionately impacted in terms of mental health issues and increased domestic labor. This research aims to investigate instructors’ and students’ perceptions of their transition to ERT. Specifically, during the transition to ERT at a research-intensive, Minority-Serving Institution (MSI), we wanted to: (1) Identify supports and barriers experienced by instructors and students. (2) Compare instructors’ experiences with the students’ experiences. (3) Explore these supports and barriers within the context of social presence, teaching presence, and/or cognitive presence as well as how these supports and barriers relate to scaffolding in STEM courses.

Results
Instructors identified twice as many barriers as supports in their teaching during the transition to ERT and identified casual and formal conversations with colleagues as valuable supports. Emerging categories for barriers consisted of academic integrity concerns as well as technological difficulties. Similarly, students identified more barriers than supports in their learning during the transition to ERT. More specifically, students described pre-existing course structure, classroom technology, and community as best supporting their learning. Barriers that challenged student learning included classroom environment, student availability, and student emotion and comfort.

Conclusions
Together, this research will help us understand supports and barriers to teaching and learning during the transition to ERT. This understanding can help us better plan and prepare for future emergencies, particularly at MSIs, where improved communication and increased access to resources for both students and instructors are key.
Real-time irradiation system using patterned light to actuate light-driven on-chip gel actuators
Authors: Koike Yuha , Kodera Shunnosuke , Yokoyama Yoshiyuki , Hayakawa Takeshi 
Published: February 2022

AbstractA light-driven gel actuator is a potential candidate for a single-cell manipulation tool because it allows cells to be manipulated while ensuring less damage. Moreover, a large number of actuators can be integrated into a microfluidic chip because no wiring is required. Previously, we proposed a method for cell manipulation using light-driven gel actuators. However, the system used in the previous work did not allow the targeted cells to be manipulated in real time because the system used in the previous work could only irradiate preprogrammed patterned light. Moreover, when a large number of gel actuators are integrated into a chip, the Gaussian distribution of the laser light source results in the response characteristics of the gel actuators varying with the location of the actuator. In this work, we constructed a system that homogenized the intensity of the patterned light used for irradiation, allowing multiple gel actuators to be driven in parallel in real time. The intensity-homogenized patterned light improved the variations in the response characteristics of the gel actuators, and as a result, we succeeded in actuating gel actuators with various light patterns in real time.