bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human umbilical cord mesenchymal stem cells regulate immunoglobulin a secretion and remodel the diversification of intestinal microbiota to improve colitis
Authors: Liu Airu , Wang Xing , Liang Xiaonan , Wang Wenxin , Li Chenyang , Qian Jiaming , Zhang Xiaolan 
Published: September 2022

BackgroundMesenchymal stem cell (MSC) therapy has emerged as a promising novel therapeutic strategy for managing inflammatory bowel disease (IBD) mainly via dampening inflammation, regulating immune disorders, and promoting mucosal tissue repair. However, in the process, the associated changes in the gut microbiota and the underlying mechanism are not yet clear.MethodsIn the present study, dextran sulfate sodium (DSS) was used to induce colitis in mice. Mice with colitis were treated with intraperitoneal infusions of MSCs from human umbilical cord mesenchymal stem cells (HUMSCs) and evaluated for severity of inflammation including weight reduction, diarrhea, bloody stools, histopathology, and mortality. The proportion of regulatory T cells (Tregs) and immunoglobulin A-positive (IgA+) plasmacytes in gut-associated lymphoid tissue were determined. The intestinal and fecal levels of IgA were tested, and the proportion of IgA-coated bacteria was also determined. Fecal microbiome was analyzed using 16S rRNA gene sequencing analyses.ResultsTreatment with HUMSCs ameliorated the clinical abnormalities and histopathologic severity of acute colitis in mice. Furthermore, the proportion of Tregs in both Peyer’s patches and lamina propria of the small intestine was significantly increased. Meanwhile, the proportion of IgA+ plasmacytes was also substantially higher in the MSCs group than that of the DSS group, resulting in elevated intestinal and fecal levels of IgA. The proportion of IgA-coated bacteria was also upregulated in the MSCs group. In addition, the microbiome alterations in mice with colitis were partially restored to resemble those of healthy mice following treatment with HUMSCs.ConclusionsTherapeutically administered HUMSCs ameliorate DSS-induced colitis partially via regulating the Tregs–IgA response, promoting the secretion of IgA, and facilitating further the restoration of intestinal microbiota, which provides a potential therapeutic mechanism for HUMSCs in the treatment of IBD.
Pre-existing anti-HCoV-OC43 immunity influences the durability and cross-reactivity of humoral response to SARS-CoV-2 vaccination
Authors: Hu Caiqin , Wang Zheng , Ren Li , Hao Yanling , Zhu Meiling , Jiang He , Wang Shuo , Li Dan , Shao Yiming 
Published: September 2022

PurposeThis study was conducted in order to properly understand whether prior seasonal human coronavirus (HCoV) immunity could impact the potential cross-reactivity of humoral responses induced by SARS-CoV-2 vaccine, thereby devising universal coronavirus vaccines for future outbreaks.MethodsWe performed enzyme-linked immunosorbent assay (ELISA) to quantify the immunoglobulin G (IgG) antibody levels to spike (S) protein and S1 subunit of HCoVs (HCoV-OC43, HCoV-HKU1, HCoV-NL63, and HCoV-229E), and ELISA [anti-RBD and anti-nucleoprotein (N)], chemiluminescence immunoassay assays (anti-RBD), pseudovirus neutralization test, and authentic viral neutralization test to detect the binding and neutralizing antibodies to SARS-CoV-2 in the vaccinees.ResultsWe found that the antibody of seasonal HCoVs did exist before vaccination and could be boosted by SARS-CoV-2 vaccine. A further analysis demonstrated that the prior S and S1 IgG antibodies of HCoV-OC43 were positively correlated with anti-RBD and neutralization antibodies to SARS-CoV-2 at 12 and 24 weeks after the second vaccination, and the correlation is more statistically significant at 24 weeks. The persistent antibody levels of SARS-CoV-2 were observed in vaccinees with higher pre-existing HCoV-OC43 antibodies.ConclusionOur data indicate that inactivated SARS-CoV-2 vaccination may confer cross-protection against seasonal coronaviruses in most individuals, and more importantly, the pre-existing HCoV-OC43 antibody was associated with protective immunity to SARS-CoV-2, supporting the development of a pan-coronavirus vaccine.
Systemic lupus erythematosus and the risk of cardiovascular diseases: A two-sample Mendelian randomization study
Authors: Huang Shuo , Huang Fugang , Mei Chunyun , Tian Fengyuan , Fan Yongsheng , Bao Jie 
Published: September 2022

BackgroundPrevious observational studies have suggested that the causal role of systemic lupus erythematosus (SLE) in the risk of cardiovascular diseases (CVDs) remained inconsistent. In this study, we aimed to investigate the causal relationship between SLE and CVDs by two-sample Mendelian randomization (MR) analysis.MethodsGenetic instruments for SLE were obtained from a public genome-wide association study (GWAS) with 4,036 patients with SLE and 6,959 controls. Summary statistical data for CVDs, including coronary artery disease (CAD), myocardial infarction (MI), atrial fibrillation (AF), ischemic stroke (IS), and its subtypes, were identified from other available GWAS meta-analyses. The inverse-variance weighted (IVW) method was used as the primary method to estimate the causal effect. The simple- and weighted-median method, MR-Egger method, and MR pleiotropy residual sum and outlier (MR-PRESSO) were provided as a supplement to the IVW method. Besides, we performed sensitivity analyses, including Cochran's Q test, MR-Egger intercept test, and leave-one-out analysis, to evaluate the robustness of the results.ResultsA total of 15 single-nucleotide polymorphisms (SNPs) were identified after excluding linkage disequilibrium (LD) and potential confounding factors. According to the IVW results, our MR study indicated that genetically predicted SLE was not causally connected with the risk of CVDs [CAD: odds ratio (OR) = 1.005, 95% confidence interval (CI) = 0.986–1.024, p-value = 0.619; MI: OR = 1.002, 95% CI = 0.982–1.023, p-value = 0.854; AF: OR = 0.998, 95% CI = 0.982–1.014, p-value = 0.795; IS: OR = 1.006, 95% CI = 0.984–1.028, p-value = 0.621; cardioembolic stroke (CES): OR = 0.992, 95% CI = 0.949–1.036, p-value = 0.707; small vessel stroke (SVS): OR = 1.014, 95% CI = 0.964–1.067, p-value = 0.589; large artery stroke (LAS): OR = 1.030, 95% CI = 0.968–1.096, p-value = 0.352]. Analogical findings could be observed in supplementary MR methods. Sensitivity analyses suggested that the causal estimates were robust.ConclusionOur two-sample MR analysis provided no evidence that genetically determined SLE was causally associated with the risk of CVDs.
Incidence and effect of secondary cardiac amyloidosis on outcomes of patients with t(11;14) multiple myeloma
Authors: Wang Jinghua , Yang Shuo , Liao Pengjun , Zeng Lingji , Ling Wei , Wan Li , Weng Jianyu , Zhong Liye 
Published: September 2022

BackgroundThe t(11;14)(q13;32) is a common chromosome translocation in multiple myeloma (MM), but its prognostic value remains controversial. Immunoglobulin light chain amyloidosis is commonly secondary to multiple myeloma, which can rapidly cause heart failure and high mortality. We aimed to investigate the prevalence of secondary cardiac amyloidosis in MM patients with t(11;14) and to evaluate its impact on survival outcomes.MethodsWe retrospectively identified 52 MM patients with t(11;14) in our center between October 2015 and April 2022. The associations between cardiac amyloidosis and clinical and biological parameters were statistically analyzed, and the impacts of concomitant of cardiac amyloidosis on survival and prognosis of MM patients with t(11;14) were also assessed.ResultsConcomitant presence of cardiac amyloidosis was observed in 15 (28.8%) of all cases. Patients with cardiac amyloidosis had significantly higher NT-proBNP (p = 0.002) and higher hs-cTnT (p &lt; 0.001), while the patients without cardiac amyloidosis had higher percentage of bone marrow plasma cells (p = 0.027), higher incidence of hemoglobin &lt;80 g/L (p = 0.021) and bone destruction (p &lt; 0.001). The median overall survival (OS) for all patients was 33.4 months after a median follow-up of 23.8 months. The amyloidosis group showed a significantly shorter OS than the non-amyloidosis group (15.3 vs. 41.8 months, p &lt; 0.001). Besides, patients harboring NT-proBNP &gt;1,800 pg/ml (p &lt; 0.001) or hs-cTnT ≧40 pg/ml (p = 0.001) or light chain (LC) only isotype (p = 0.033) had a significantly shorter mean OS compared with patients with lower NT-proBNP or hs-cTnT or other M-protein isotype. Univariate analyses showed that NT-proBNP &gt;1,800 pg/ml, hs-cTnT ≧40 pg/ml, LC only isotype, and concomitant presence of cardiac amyloidosis were independently associated with shorter OS, while NT-proBNP &gt;1,800 pg/ml still retained the prognostic value for OS in multivariate analyses.ConclusionThe t(11;14) MM patients with coexisting cardiac amyloidosis may represent a distinct clinical entity that confers a poor outcome. These findings may have important clinical and biological implications.
Forearc tectonics and volcanism during the Devonian–Carboniferous evolution of the North Patagonian segment, southern Chile (41,3°S)
Authors: Palape C. , Quezada P. , Bastías J. , Hervé F. , Reyes T. , Veas M. , Vildoso F. , Calderón M. , Theye T. , Fuentes F. , Chiaradia M. 
Published: September 2022

Late Paleozoic to early Mesozoic subduction complexes formed during the evolution of southwestern Gondwana and extensively crop out along the Chilean continental margin. Recent findings in northern Patagonia (40°–43°S) revealed that accretionary processes were active since the Devonian when enhanced lithosphere stretching in the forearc led to the formation of Chaitenia island arc. The extension in the crust consecutively developed a backarc basin, which culminated during a compressive episode that re-amalgamated the Chaitenia island arc with the margin. This episode produced intermediate grade metamorphism in the sedimentary rocks that were formed throughout the extension. To constrain the tectonic evolution of these processes, we combined petrology, structural analysis, whole-rock geochemical, and whole-rock isotopic tracing (Sr-Nd-Pb) data along with thermodynamic modelling. Two petro-tectonic domains are here defined. The Western Coastal Range Domain is composed of Carboniferous to Permian metapsammopelitic rocks, which are mainly schists with a metasedimentary Carboniferous protolith exhibiting a penetrative northeast to southwest dipping main foliation associated with basal accretion. The Eastern Coastal Range Domain is comprised by garnet micaschists, metabasalts, metarhyolites, and metasandstones. This unit is folded by three ductile structures: The first is related to rootless isoclinal folds, the second is associated with kilometric scale west-verging tight folds, and the third is associated with west-verging cylindrical folds. The volcanic rocks of this domain are comprised by middle Devonian alkaline metarhyolites and metabasalts with enriched-MORB and normal-MORB affinities. Trace element composition suggests that the metabasalts formed through shallow melting in an extensional setting over a supra-subduction zone. Nd and Pb isotope data point to a mantle source change for the basaltic melts from an EM1-like to a DM-like and are interpreted to reflect the embryonic to mature evolution of the early Devonian to Carboniferous backarc system. The calculated P–T evolution of the garnet micaschists follows: 1) a clockwise IP–IT prograde Barrovian path, 2) an isobaric thermal increase at ∼7 kbar and 540°C, and 3) an adiabatic decompression. Finally, after the metamorphism, these rocks were uplifted by thrusting processes that probably occurred during the late Permian.
Nature and origin of anorthosite enclaves within Proterozoic granite of Chotanagpur Granite Gneiss Complex of Eastern India
Authors: Negi Priyanka , Saikia Ashima , Ahmad Mansoor , Upadhyay Dewashis , Akhtar Salim 
Published: September 2022

The Central Indian Tectonic Zone (CITZ) is a Proterozoic suture along which the Northern and Southern Indian Blocks are inferred to have amalgamated, forming the Greater Indian Landmass. The Chotanagpur Granite Gneiss Complex (CGGC) represents the eastern extension of the CITZ and exposes several granite plutons. Enclaves of diverse origin are present as minor constituents within these granitic bodies. This study reports new major and trace element data for anorthosite enclaves hosted within granites in the Pattharkatti and Rajgir area from the northern margin of CGGC to get modern insights into the petrogenesis of anorthosites. Anorthosite enclaves show sharp contact with the host granite. They contain cumulus plagioclase (An87–94), intercumulus amphibole (magnesiohornblende and ferrotschermakite), and biotite (Mg-biotite and phlogopite) along with minor iron oxides. Amphibole crystallization pressure and temperature are constrained between 0.5 and 6.4 kbar and 653–780°C for the anorthosites. The studied anorthosites display a very gradual and steady increase in whole-rock rare earth element (REE) contents from Lu to La [(La/Yb)N = 1.22–13.08]. They also show a sharp decline in Fe2O3(t) and MgO, whereas Al2O3 increases with increasing silica contents from 45.69 to 51.16 wt%. In the chondrite normalized REE diagram, plagioclase exhibits LREE enriched patterns with strong positive Eu anomaly. The composition of parental liquid for anorthosite from the study area was estimated by adopting the equilibrium distribution method. Parental melt curves from Sm to La are near parallel and constrained broadly between trapped melt fractions (TMF) = ∼5%–15%. Anorthosites of the study area may have formed from the plagioclase-saturated basaltic melt.
3D deep electrical structure and seismogenic environment in the western section of the Zhangjiakou-Bohai fault zone
Authors: Peng Yuanqian , Sun Xiangyu , Zhan Yan , Zhao Lingqiang , Luo Quanxing , Liu Xuehua , Ran Zhijie , Wang Lisen 
Published: September 2022

The Zhangjiakou-Bohai fault zone (ZBFZ) is an important NW-trending active tectonic zone in North China. The western section of ZBFZ is characterized by frequent moderate and strong earthquakes. This is a typical tectonic area for studying seismic and volcanic activities. The three-dimensional (3D) deep electrical structure of the region was revealed by using 3D electromagnetic inversion of 143 magnetotelluric stations. The results suggest that the deep electrical structure in the western part of the ZBFZ is laterally heterogeneous. To the north of the Shangyi-Chicheng Fault (F4), the upper crust has a high resistivity structure (R), corresponding to the exposed Hanuoba basalts and metamorphic rocks. To the south of F4, high and low resistivity zones alternate, which is attributed to the complex faults and basin-range structures. In the middle crust, the low-resistivity structures (C1 and C2) gradually appear in the northwest and southeast of the study area, showing the characteristics of a high-resistivity structure sandwiched between two low-resistivity structures. In the lower crust, the two low-resistivity structures (C1 and C2) gradually join each other and the whole region becomes characterized by low resistivity. The Shangyi-Chicheng Fault (F4), the northern margin fault of the Huai’an Basin (F7), and the northern margin fault of the Huaizhuo Basin (F11) are definite electrical difference zones, which are connected to a low-resistivity layer in the crust. The results indicate that the focal depth of the 1998 M6.2 Zhangbei earthquake was between 12 km and 15 km and its seismogenic fault was the Dahezhen-Hailiutu Fault (F3), which is concealed beneath the Hanuoba basalt area. It is speculated that owing to the neotectonic activities, the magma originated in the asthenosphere mantle rose and erupted on the surface near Datong, forming the Datong volcanic group. Then the magma continued to migrate and overflow along with the fractures in the NE direction, forming the Hannuoba basalts in the Zhangbei area. The existence of mantle thermal material in this area led to the migration of accompanying fluids to the middle and upper crust, resulting in fault sliding and frequent occurrence of moderate and strong earthquakes in this region.
Theoretical analysis of the reasonable support capacity based on voussoir beam structure for a given load of the loose body under an extremely close goaf
Authors: Ren Yuqi , Li Yang , Lei Xinghai , Ou Xiangji , Wang Nan , Li Guoshuai , Yang Kunpeng 
Published: September 2022

Given the growing popularity of fully mechanized longwall mining, hydraulic shields have emerged as the most crucial pieces of equipment whose rated support capacities are a significant assurance for safe extraction of coal seams. Based on the extraction in the 1,692 panel of the No.9 coal seam in Qianjiaying coal mine, a detailed study of the reasonable shield support capacity under an extremely close goaf was conducted using field measurements, theoretical analyses, and system development. The results show that the ZY4800-13/32 shield employed in the 1,692 panel is unreasonable owing to its large surplus coefficient of support capacity and low support utilization rate. The voussoir beam structure is established for a given load of the loose body to calculate the shield support capacity when the lower coal seam is extracted under an extremely close goaf. The calculated shield support capacity required for the No.9 coal seam was 3,560.03 kN, so the rated support capacity should be 4,000 kN. A calculation and analysis scheme for the shield support capacity under the given load of loose body was developed using a GUI in Matlab, and this scheme was used to examine the influence degrees of the relevant parameters of the immediate roof, main roof, and loose body on the shield support capacity. The thickness of the main roof was found to have the greatest influence on the shield support capacity. Finally, presplitting the main roof was proposed as an effective method for controlling the breaking length and reducing the shield support capacity based on existing research.
Economic evaluation of production capacity for natural gas hydrate industrial exploitation in the South China Sea
Authors: Wu Linqiang , Zhang Tao , Zhang Hongtao , Sui Yuzhang , Yu Xinghe , Miao Miao 
Published: September 2022

Natural gas hydrate (NGH) is a worldwide strategic and prospecting commercial resource in the 21st century. The industrialization of NGH has great strategic significance for the achievement of peak carbon dioxide emissions and carbon neutrality. Prior to its industrialization, an economic evaluation of production capacity for each well per day should be conducted to determine whether it is profitable at different given gas prices. In this study, a new hybrid method based on the discounted cash flow (DCF) method and the energy return on investment (EROI) method is used to estimate the economic production rate of NGH exploitation at four different gas price scenarios. The results show that the lowest production rate to make NGH exploitation economic ranges from 1.96 to 29.60 × 104 m3/d/well. With the change in the number of wells, gas–water ratio, gas price, decreasing rate in production cost, and sensitivity analysis are carried out. It shows that all these key factors have a significantly negative effect on the economic production rate initially, and then the sensitivity to the economic production rate will become lower and lower with the rising value of each key factor.
Quantitative characterization of organic and inorganic pores in shale based on FIB-SEM
Authors: Zhao Jianpeng , Chen Hui , Zhang Jinyan , Zhang Wenjiao , Chen Gang 
Published: September 2022

The pore structures of shale in the Shahejie formation of Dongying depression were quantitatively characterized by FIB-SEM three-dimensional imaging technology. FIB-SEM was used to obtain high-resolution SEM images and through image registration, geometric correction and image segmentation, the organic pores and inorganic pores in the shale were distinguished. The pore space was converted into a structured pore network model via maximal ball method. Then, the pore size distributions and volume contributions of shale organic pores and inorganic pores, and the coordination number of the total pore network model were statistically analyzed via the pore network model. The results showed that due to the low thermal maturity of organic matter in the Shahejie formation, fewer organic pores were observed in the shale samples, and the number of pores was dominated by inorganic pores. Statistical analysis of the pore network model indicated that the studied shale samples considerably included mesopore (size of 2–50 nm) which accounted for 61.1%, but their contribution to the total pore volume was small (4.2%). Macropores (&gt;50 nm) accounted for a relatively small proportion (38.9%) in number, but they provided the main storage space (95.8%) for the shale oil and gas. The volume contribution of pores to the total pore space at a certain bin size is controlled by the combination of pore number and the pore size. The coordination numbers of the two samples were mainly 0 and 1, indicating that the pore connectivity was poor and isolated pores accounted for most part. These results are critical for further reliable petrophysical simulations based on shale digital rocks as well as for the accurate understanding of their petrophysical properties.
Supersonic shear wave imaging of the tibial nerve for diagnosis of diabetic peripheral neuropathy: A meta-analysis
Authors: Chen Yuping , Duan Honghong , Huang Lichun , Jiang Zhengrong , Huang Huibin 
Published: September 2022

BackgroundDiabetic peripheral neuropathy (DPN) is the most common diabetes-associated complication and imposes a significant burden to healthcare systems. Thus, early diagnosis of DPN is extremely critical for management and outcome of diabetic patients. Supersonic Shear Wave Imaging (SSI) enables the noninvasive measurement of nerve stiffness. However, previous studies on SSI in the diagnosis of DPN were limited in sample sizes and reported various results. In this meta-analysis, we aimed to obtain comprehensive evidence on the value of tibial nerve stiffness measurement by SSI in the diagnosis of DPN.MethodsA comprehensive literature search in English and Chinese electronic database was conducted for studies (published until January 25, 2022) that investigated the diagnostic performance of tibial nerve stiffness measurement by SSI for detecting DPN. Summary receiver operating characteristics (SROC) modelling was constructed to conduct the meta-analysis of diagnostic accuracy of SSI for detecting DPN.ResultsFinally, a total of 12 eligible studies with 1325 subjects were included for evaluation, and a meta-analysis was conducted to evaluate the diagnostic performance of tibial nerve stiffness measurement by SSI for detecting DPN. For tibial nerve stiffness measurement by SSI, the summary sensitivity and specificity for the diagnosis of DPN were 80% (95% confidence interval [CI]: 73%–86%) and 86% (95% CI: 82%–89%), respectively. The summary area under the ROC curve (AUROC) value of the SROC was 0.90 (95% CI: 0.87–0.92), for diagnosing DPN. A subgroup analysis of 11 SSI studies from China revealed similar diagnostic performance, with a summary sensitivity of 79% (95% CI: 72%–85%), specificity of 86% (95% CI: 82%–89%) and summary AUROC value of the SROC of 0.90 (95% CI: 0.87–0.92) for diagnosing DPN.ConclusionsOur meta-analysis suggests that a tibial nerve stiffness measurement by SSI shows good performance in diagnosing DPN and has considerable potential as a noninvasive tool for detecting DPN.