bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa
Authors: FATMAWATI FATMAWATI , SIBERO MADA TRIANDALA , TRIANTO AGUS , WIJAYANTI DIAH PERMATA , SABDONO AGUS , PRINGGENIES DELIANIS , RADJASA OCKY KARNA 
Published: November 2022

Abstract. Fatmawati F, Sibero T, Trianto A, Wiyanti DP, Sabdono A, Pronggenies D, Radjasa OK. 2022. The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa. Biodiversitas 23: 5258-5266. Gracilaria verrrucosa is producer of agar, a polysaccharide, that widely used in pharmaceutical and food industries. However, several studies have shown that the secondary metabolite of this seaweed is not potential as an antimicrobial and antioxidant agents. This study aimed to determine the antibacterial and antioxidant properties of methanolic extract of G. verrucosa after fermentation using marine yeast Hortaea werneckii SUCCY001. The samples were fermented based on different optimization times, specially in three, six and nine days. Samples were extracted using methanol by maceration method. Then the extracts were tested for antimicrobial potential against the several human clinical pathogens namely Bacillus cereus, Bacillus subtilis, Escherichia coli, Micrococcus luteus and Pseudomonas aeruginosa. The antioxidant potential was analyzed using the 1,1-diphenyl-2-picrylhydracyl (DPPH) and 2,2-Azinobis 3-ethyl benzothiazoline 6- sulfonic acid (ABTS) method while total phenolic content (TPC) was measured using Folin-Ciocalteu. Secondary metabolites were characterized using thin layer chromatography (TLC) and GC-MS. TLC plate visualization using UV light, DPPH, FeCl3 and vanillin-sulfuric acid reagents. The results showed that the extract fermented for nine days was able to inhibit the growth of P. aeruginosa and M. luteus at a concentration of 2 mg/mL. The highest antioxidant activity was found in the nine days fermented extract with an RSA value of 21.9% ± 0,0001 for DPPH and 12.53 % ± 0.002 for ABTS. Furthermore, the highest total phenolic content was found in the nine days fermented extract of 7.57 GAE/mg. The results of characterization using TLC showed the presence of phenolic compounds, terpenoids and antioxidants in fresh seaweed extracts, autoclaved fresh seaweed, three days of fermentation, six days and nine days. Based on this research, it can be seen that the addition of marine yeast starter H. werneckii SUCCY001 was able to increase the antibacterial and antioxidant activity of G. verrucosa.
The impact of fishermen’s conflict on the sustainability of crab (Portunus pelagicus) resources in the coastal areas of Maros District, South Sulawesi, Indonesia
Authors: Daris Lukman , Massiseng Andi Nur Apung , Fachri Mardiana Ethrawaty , Jaya Jaya , Zaenab ST 
Published: November 2022

Abstract. Daris L, Massiseng ANA, Fachry ME, Jaya, Zaenab ST. 2022. The impact of fishermen’s conflict on the sustainability of crab (Portunus pelagicus) resources in the coastal areas of Maros District, South Sulawesi, Indonesia. Biodiversitas 23: 5278-5289. Using environmentally unfriendly fishing gear causes damage to coral reefs and seagrass, which are the habitat of crabs. The prohibition on using ecologically unfriendly fishing gear, such as mini trawl, in catching crabs and shrimps in the coastal area of ??Maros District, South Sulawesi, Indonesia, has triggered horizontal conflicts between traditional fishermen from 1985 until now. This study aims to identify the types of fishing conflicts and analyze the distribution of conflict areas and the maximum fishing rate that could threaten the sustainability of crab (Portunus pelagicus) resources. This research was conducted from February to July 2022 in the coastal area of ??Maros, South Sulawesi Province, Indonesia. The type of data is primary and secondary data. The data collection methods used in this study were observation, interviews, and Participatory Rural Appraisal (PRA). The analysis data used qualitative by reducing and presenting data obtained from observations, interviews, and PRA, as well as spatial analysis and Geographic Information Systems (GIS) to describe the distribution map of fishermen's conflicts. The results showed that the types of conflicts between fishermen using crab resources were agrarian conflicts, technological conflicts, and perception conflicts between fishermen using trammel nets and traps and fishermen using mini trawls. Conflicts between fishermen in resource utilization crabs occur between ??2.71-3.26 km from the coast during dark moons and 2.17-3.26 km during bright moons. Fishing conflicts have an impact on decreasing crab catches from 2015 until 2021, where the fishing effort (trip) has exceeded the sustainable fishing effort (MSY) by 690 trips/year with a total catch of 1232.3 tons/year.
Taxonomy and molecular phylogeny analysis of the genus Diogenes “Edwardsii group” (Decapoda: Anomora: Diogenidae) based on mitochondrial DNA sequences
Authors: FITRIAN TYANI , MADDUPPA HAWIS , ISMET MEUTIA SAMIRA , RAHAYU DWI LISTYO 
Published: November 2022

Abstract. Fitrian T, Madduppa H, Ismet MS, Rahayu DL. 2022. Taxonomy and molecular phylogeny analysis of the genus Diogenes “Edwardsii group” (Decapoda: Anomora: Diogenidae) based on mitochondrial DNA sequences. Biodiversitas 23: 5302-5313. Many variations of morphological characters differ from one species to another, showing high interspecific variation within the genus Diogenes Dana, 1851. Subtle morphological differences among certain species make it difficult to identify diagnostic characters. Although the genus is very speciose, the study on the molecular is poorly reported. This study aimed to evaluate the taxonomy and phylogeny of the genus Diogenes using morphological and molecular features. The molecular phylogeny of 15 species of Diogenes included in the "Edwardsii group" was constructed based on two mitochondrial genes COI and 16S rRNA. The results of the phylogeny tree construction based on concatenated mitochondrial genes COI and 16S rRNA mtDNA formed 5 clades of monophyletic. Phylogenetic relationships of Diogenes species based on concatenated mitochondrial genes COI and 16S rRNA sequenced suggest that the morphological and molecular analyses of the species in this study corroborated one another. Among 15 species studied, three species were challenging to identify due to their resemblance to other species traits. The phylogenetic tree demonstrates that the three taxa denoted by 'sp.' were different from the known species of Diogenes so far. Diogenes sp.1 was closely related to Diogenes moosai Rahayu & Forest, 1995, Diogenes sp.2 resembles Diogenes laevicarpus Rahayu, 1996, while Diogenes sp.3 was closely related to Diogenes goniochirus Forest, 1956.
Alcohol consumption and related disorders in Iran: Results from the National Surveillance of Non-Communicable Diseases’ Survey (STEPs) 2016
Authors: Rezaei Negar , Ahmadi Naser , Shams Beyranvand Mehran , Hasan Milad , Gohari Kimiya , Yoosefi Moein , Djalalinia Shirin , Saeedi Moghaddam Sahar , Modirian Mitra , Pazhuheian Forough , Mahdavihezaveh Alireza , Moradi Ghobad , Delavari Farnaz , Larijani Bagher , Farzadfar Farshad 
Published: November 2022

Background
Alcohol consumption is a public health concern which is illegal in Iran. Moreover, due to cultural and religious beliefs, the available population-based research findings on alcohol consumption are inadequate. We aimed to provide an estimate on alcohol consumption using a large-scale population-based survey in Iran.

Materials and methods
The National Surveillance of Non-Communicable Risk Factors in Iran was a population-based survey conducted in 2016. The epidemiologic distribution of alcohol consumption and its related disorders were assessed using weighted survey methods and multiple logistic regression models. Age standardized rates were calculated using Iran’s national population census in 2016.

Results
At the national level, the prevalence rates of lifetime and current alcohol consumption were 8.00% (95% CI: 7.67–8.32) and 4.04% (95% CI: 3.81–4.27), respectively. The highest prevalence was reported among 25 to 34 year-olds. Individuals of higher socioeconomic status consumed significantly greater levels of alcohol. At provincial level, the highest and lowest percentages of the current alcohol drinking rates in Iran’s provinces were, 23.92% (95% CI: 17.56–30.28) and 0.4% (95% CI: 0–1.18) in males, 1.58% (95% CI: 0.22–2.94) and 0% in females, respectively. In urban regions, the highest alcohol consumption rate was more than 22 times greater than the lowest alcohol consumption rate. Current alcohol drinkers were 2 times more prone to injury as compared to nondrinkers (ORadj: 2.0, 95%CI: 1.7, 2.3).

Conclusion
In Iran, the prevalence of alcohol consumption is low, although there is a considerable variation of alcohol consumption at provincial level as well as in different gender groups. Therefore, preventive WHO—recommended measures should be adopted more seriously by vulnerable groups.
Flora of the former Kolokolnikov estate (Tyumen)
Authors: Voronova O. G. , Patrakeeva E. V. 
Published: November 2022

The article presents for the first time data on the species diversity of vascular plants growing on the territory of the former estate of the Kolokolnikovs, which has a history of formation for a century and a half and is today a place of rest for the citizens of Tyumen. In 2021, most of the study area was classified as a natural monument of regional significance «Lesopark (Parkland) Zatyumensky». For the spring-summer period 2021, 182 species of vascular plants belonging to 136 genera, 56 families were identified. Among the leading families: Asteraceae (22 species), Rosaceae (21), Poaceae (19), Fabaceae (14), Brassicaceae (9), Salicaceae (8), Caryophyllaceae (7), Apiaceae (6), Ranunculaceae (5), which accounted for 61 %. The arboreal layer is dominated by Betula pendula and Tilia cordata, and the grass-shrub layer is dominated by Aegopodium podagraria, Centaurea scabiosa, Geranium sibiricum, Glechoma hederacea, and Poa pratensis. On the territory there are species listed in the Red Book of the Tyumen region: Tilia cordata and Cerasus fruticosa (Prunus fruticosa). In a comparative analysis with herbarium funds, 33 plant species were not identified in the modern flora of the study area. According to the biomorphological analysis according to the system of life forms of K. Raunkier, most plants belong to hemicryptophytes (40.7 %), I. G. Serebryakova - long-rhizomatous polycarpics (21.5 %), O. V. Smirnova et al. - mono-centric type (52.2 %).
Protected species of the family Liliaceae Juss. on the territory of the Altai Mountain Country
Authors: Zholnerova E. A. , Zaikov V. F. , Vaganov A. V. 
Published: November 2022

Species of the family are predominantly flowering plants. In this regard, populations suffer not only from grazing, haymaking, economic development of land, but also from the collection of flowers and bulbs for bouquets and sale. To assess the proportion of rare and endemic species of the family, an analysis of the red books of Kazakhstan, China, Mongolia, Russia and its subjects included in the territory of the Altai mountainous country was carried out. A table has been compiled indicating the categories of rarity for each protected species. Almost half of the species of the Liliaceae family in the territory of the AMC have a conservation status, a third of which are endemic. The DIVA-GIS 7.5 program was used to map and analyze the data on the distribution of representatives of the Liliaceae family in the botanical and geographical regions of the AMC. Maps were compiled based on the inventory of the ALTB, NS (NSK), MW, E, CAS, PE, B, and G collections. Also, as part of the study, 54 sheets of species of the genus Gagea were digitized with subsequent integration into GBIF. It is necessary to comprehensively study the species, their exact ranges and take immediate measures to conserve taxa that are in the rarity category 1 (endangered) and 2 (vulnerable).
Some rare and new plants in the National Parks of Northern Tien-Shan (Kazakhstan)
Authors: Ivashchenko A. A. , Kaparbai R. E. , Almabek D. M. , Arynov B. B. , Abidkulova K. T. 
Published: November 2022

The article gives information on the distribution of four rare species (Hepatica falconeri, Kaufmannia semenovii, Neuroloma beketovii, Gymnospermium altaicum) with brief abundance data and state of populations. This studies based on the materials of many years researches carried out by the authors on the territory of two state national natural parks (SNNP) of the Northern Tien Shan (Ile Alatau and Kolsai Kolderi). Nine species (Gagea turkestanica, Bunias orienta-lis, Viola dissecta, Clinopodium integerrimum, Melissa officinalis, Sambucus nigra, Hieracium pilosella, Lapsana intermedia and Xanthium spinosum) were first found on surveyed areas, which allows supplementing the existing publications on the flora of the national parks Ile Alatau and “Kolsai Kolderi”, as well as the Kungei Alatau ridge. Three of them (Gagea turkestanica, Viola dissecta, Clinopodium integerrimum) are native to this area, which indicates that the flora of the region is not well studied. Six others (Bunias orientalis, Melissa officinalis, Sambucus nigra, Hieracium pilosella, Lapsana intermedia and Xanthium spinosum) are alien species, which confirms the ongoing process of synanthropization of the flora of the Northern Tien Shan even within specially protected natural areas.
Vitality of alasic cenopopulations of Hordeum brevisubulatum (Trin.) Link
Authors: Kardashevskaya K. V. , Kardashevskaya V. E. 
Published: November 2022

The vital structure of cenopopulations (CP) is one of the main indicators of the state of the CP, which really reflects vitality and resistance to stress. In 2020-2021, the vital structure of the CP of polycarpic, rhizomatous-friable grass Hordeum brevisubulatum (Trin.) Link, one of the dominants and important resource components of the alasny meadows in the Churapchinsky district of Yakutia, was studied. 32 morphological parameters of generative individuals were studied. The repetition by individuals in each CP is 30-fold. The assessment of the vitality of individuals and the CP as a whole was carried out according to the method of Yu. A. Zlobin, supplemented by A. R. Ishbirdin and M. M. Ishmuratov. Individuals of the CP of the аЫ middle belt have parameter values lower than those of the CP of the lower belt, but higher than in the CP of the upper belt of the аЫ. Studies established that in the CP of the species there are individuals of all three classes of vitality: a-higher, b-middle and c-lower. In general, over the years of research, the vital structure of the аЫ CP of the Churapchinsky district has undergone some changes. The middle class of vitality and a thriving type of cenopopulation prevail. The species is adapted to the limited soil and climatic resources of the alas, but is most viable in the lower belts of the alas. The vital state of the CP is affected by the position of the CP in a certain zone of alas and the moisture content of the year.
Taxonomic diversity and structure of phytoplankton in different-type lakes of Kulunda basin (Altai krai)
Authors: Kosacheva Yu. N. , Mitrofanova E. Yu. 
Published: November 2022

In the phytoplankton of different-type lakes of Kulunda basin 87 species and 1 variety of algae from 6 systematic divisions were identified: Cyanobacteria, Bacillariophyta, Cryptophyta, Euglenophyta, Chlorophyta and Ochrophyta. The most diverse in the number of families, genera and species is the Cyanobacteria (35 species, or 40.2 % of the total number of species), as well as Chlorophyta (26, or 29.9 %), Bacillariophyta (17, or 19.5 %). Most of the taxa ranked above the family belong to Bacillariophyta (2 classes, 3 subclasses, 9 orders) and Chlorophyta (3 classes, 8 orders). The two divisions are not numerous, they have only 2 (Cryptophyta) and 3 (Ochrophyta) species. The largest family is Oscillatori-aceae with 4 genera and 13 species. The leading genera include 5 genera, and 3 of them are from Cyanobacteria, 1 genus each belong to Chlorophyta (Ulothrix) and Bacillariophyta (Nitzschia). According to the results of the study, the dependence of the number of species in phytoplankton on the average mineralization and Wolf Number (the measure of solar activity) for salt lakes in Kulunda basin was revealed.
The role of the Pamir Botanical Garden in the enrichment of the assortments of conifers in the Western Pamirs
Authors: Kurbonbekov J. Sh. 
Published: November 2022

This article presents some results of cuttings of coniferous plants in the conditions of the Western Pamirs, on the example of the Pamir Botanical Garden, as well as enriching the range and prospects for their use. Since under natural conditions, the diversity of conifers in the Pamirs is very small and mainly species of the juniper genus grow here, in the Pamir Botanical Garden, for many years, research has been carried out to study the introduced coniferous plants and their adaptation to the conditions of this territory. In the course of the study on introduction, the optimal cutting time was determined for species of spruce, biota and others. It is shown that the spring period of grafting in comparison with the summer period is the most optimal for the conditions of the Western Pamirs. It was determined that western thuja (Thuja occidentalis) has a very good percentage of survival (60 %) compared with prickly spruce and European spruce, which are inferior to it by 15-20 %. The article also gives a brief description of conifers that have already been acclimatized in the conditions of the Western Pamirs and there is a prospect of their use for landscaping and soil protection in different parts of this territory. These include species of the genus biota, thuja, spruce and others.
The species composition of weeds in sunflower crops in the steppe zone of the Krasnodar Territory
Authors: Luneva N. N. , Zakota T. Yu. 
Published: November 2022

In sunflower crops cultivated in the steppe zone of the Krasnodar Territory, 92 species of weeds were identified. The core of the weed component of agrocenoses consists of 2 especially active species - Amaranthus retrof-lexus L. and Chenopodium album L., 5 highly active species - Ambrosia artemisiifolia L., Convolvulus arvensis L., Abu-tilon theophrasti Medik., Echinochloa crusgalli (L.) Beauv., Persicaria maculosa S.F. Gray, 2 medium-active species Cir-sium setosum (Willd .) Bess. and Vicia cracca L. and 2 fairly active species Sinapis arvensis L. and Lathyrus tuberosus L. These are the types that should be paid special attention to while developing protective measures. In addition, 22 inactive species and 59 inactive species were identified, the presence of which in sunflower crops is due to a decrease in the level of technology of this crop cultivation. The territorial totality of weeds of the ecotope of segetal habitats is a segetal flora, one of the subdivisions of which is the partial flora of the ecotope under the cultivation of sunflower culture. The species composition of the segetal flora is characterized by stability, therefore it can be predicted for a certain time perspective. The activity of species changes with the change of crops in the crop rotation scheme, which significantly increases the role of phytosanitary monitoring.
Anatomical structure features of underground organs of Epilobium hirsutum L. (Onagraceae) in habitats with variable watering
Authors: Ovchinnikova Yu. A. , Shabalkina S. V. 
Published: November 2022

Habitats with variable watering are characterized by fairly stable illumination during the growing season, while moisture availability varies. Epilobium hirsutum L. also grows under such conditions. It is a polycarpic; clearly poly-centric juvenile of vegetative origin; geophyte; hygrophyte. The aboveground organs of this species are used for medicinal purposes, therefore the anatomical structure of the stem and leaf is described earlier. The purpose of this study is to identify the features of the internal structure of underground organs in connection with the conditions of variable watering. The underground organs of vegetatively arisen individuals are formed by sympodially growing transitional formations from the stolon to the rhizome with adventitious roots. During the study, transverse sections of the internodes of the geophil-ic part of the shoot and adventitious roots were made, and the linear dimensions of the structures were measured. It was found that the air-bearing cavities and the storage parenchyma, which occupies most of the shoot axis, are developed in the geophilic site; conductive and mechanical tissues are poorly formed. The revealed features emphasize the adaptability of the geophilic site to the implementation mainly of the functions of the supply of substances and air, vegetative reproduction and proliferation. Adventitious roots of two types, which differ in location, structure, age and functional heterogeneity.
Spatial structure of Iris pumila L. cenopopulations in the Volgograd region
Authors: Suprun N. A. 
Published: November 2022

One of the modern environmental problems is the reduction in the number of rare plant species due to an increase in anthropogenic pressure on natural phytocenoses. Cenopopulation studies of Iris pumila L. will make it possible to study the real state of natural coenopopulations, which is the result of the intrapopulation distribution of individuals. This article is devoted to the issue of the spatial structure of Iris pumila populations in the Volgograd region. The studies were carried out in 25 coenotic populations within the Volgograd region at 11 geographical points. The total area of surveyed populations was about 923,000 m2. The article discusses the main distribution types of a rare species individuals in natural populations, and also gives their characteristics. Ecological and coenotic studies of a rare species at the regional level are presented. The life cycles of Iris pumila have been determined. The data on the vitality structure of the Iris pumila cenopopulation are given, the ratio of individuals of different levels of vitality in the population is an important characteristic, which gives an assessment of the level of population viability in specific habitat conditions. The average density of generative individuals in the population is 92.6 pcs/m2. The number of individuals of Iris pumila in most local populations is stable. Reproduction of plants and renewal of individuals is quite stable. Under the influence of anthropogenic activity, individuals in populations age, and renewal decreases. At present, the studied cenopopulations are in stable good condition.