bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of MRP8/14 in clinical practice as a predictor of outcome after methotrexate withdrawal in patients with juvenile idiopathic arthritis
Authors: Sumner Emma J. , Almeida Beverley , Palman Jason , Bale Peter , Heard Clare , Holzinger Dirk , Roth Johannes , Foell Dirk , Robinson Emily , Ursu Simona , Wallace Chris , Gilmour Kimberly , Wedderburn Lucy R. , Ralph Elizabeth 
Published: April 2022

Abstract
The objective of this study was to determine the effectiveness of MRP8/14 as a predictor of disease flare in patients with juvenile idiopathic arthritis (JIA) following the withdrawal of methotrexate (MTX) in a routine clinical setting. All MRP8/14 tests performed at a single centre in a 27-month period were considered for analysis. Patients were assessed against criteria for inactive disease and subsequent disease flare. Decisions on whether or not to stop treatment were recorded. MRP8/14 results were assessed in conjunction with clinical information. Clinicians were also surveyed to investigate if MRP8/14 influenced their decision to discontinue MTX where this was available at that time point. One hundred four cases met the inclusion criteria during the study period. Although there was no significant difference in flares between patients with an elevated or low MRP8/14 value, in those who stopped MTX (n = 22), no patients with a low MRP8/14 (≤ 4000 ng/ml) result flared (follow-up time 12 months). Clinicians reported that for patients with clinically inactive disease and an elevated MRP8/14 result (> 4000 ng/ml), none would advise withdrawal of MTX. Low MRP8/14 was interpreted favourably when considering stopping MTX treatment in patients with JIA. Implementation of MRP8/14 testing has changed clinical practice at this centre. However, the observation that some patients in our cohort who had an elevated MRP8/14 value did not flare after stopping MTX for non-disease-related reasons highlights the need for further biomarkers to predict the risk of flare off medication in JIA and aid clinicians in treatment decisions.

Key Points• First study of serum MRP8/14 measurement in clinical practice to inform treatment decisions in patients with JIA.• No patients with a low MRP8/14 test result went on to suffer a disease flare in 12 months of follow follow-up.• Further biomarkers are needed to predict the risk of flare off medication in JIA and treatment decisions.
Ventriculostomy-related intracranial hemorrhage following surgical and endovascular treatment of ruptured aneurysms
Authors: Lenschow Moritz , von Spreckelsen Niklas , Telentschak Sergej , Kabbasch Christoph , Goldbrunner Roland , Grau Stefan 
Published: April 2022

AbstractEndovascular therapy of ruptured aneurysms is regularly accompanied by periprocedural heparinization and requires the use of periprocedural antiplatelets in more complex cases. This raises concerns regarding increased bleeding risks in the case of frequently required ventriculostomy. The aim of this study was to analyze risk factors for ventriculostomy-related intracranial hemorrhages (VS-ICH) in endovascular or surgical treatment of ruptured aneurysms with a focus on antithrombotic therapy. In this retrospective analysis, we included patients admitted to our institution over a 12-year period who had received at least one ventriculostomy due to subarachnoid hemorrhage-related hydrocephalus. Patients were dichotomized into an endovascular and surgical group and rates of VS-ICH were compared. Risk factors for VS-ICH were assessed in uni- and multivariate analyses. A total of 606 ventriculostomies were performed in 328 patients. Within the endovascular group, antiplatelet therapy was used in 44.8% of cases. The overall rate of ventriculostomy-related intracranial hemorrhage was 13.1%. Endovascular treatment was associated with a higher rate of VS-ICH compared to surgical treatment (p = 0.011), but not in cases without antiplatelet therapy (p = 0.166). Application of any antiplatelet therapy (odds ratio, 2.647 [95% confidence interval, 1.141–6.143]) and number of ventriculostomies (odds ratio, 2.513 [95% confidence interval, 1.859–3.395]) were independent predictors of ventriculostomy-related hemorrhages. Our findings indicate an increased risk of VS-ICH in the endovascular group if administration of antiplatelets was required. While this aspect has to be included into treatment decision-making, it must be weighed against the benefits of endovascular techniques.
Ontological congruence, discipline and academic identity in university schools of nursing
Authors: Barrow Mark 
Published: April 2022

AbstractNursing is an old vocation but is relatively new to the academy, with schools of nursing being established in Western universities in the late twentieth and early twenty-first centuries. Their establishment was presaged by earlier moves of the preparation of pre-registration nurses from apprenticeships served in hospitals to tertiary education institutions (mainly community colleges and polytechnics) during the last quarter of the twentieth century. Preparation for a life in the professions has been a feature of universities since their inception. Nevertheless, changing a university’s offerings is contested, and new disciplines and the new academics within them often struggle to establish their legitimacy within the academy. This paper challenges contemporary accounts of nursing as a discipline the weak disciplinary boundaries of which undermine its place in the academy and hamper nurse academics’ development of an academic identity. Drawing on data from interviews with nursing academics, the paper discusses the ways in which the participants are, by their own actions, devising, amending and reinforcing the structures, code, rules and conceptual frameworks of the Nursing discipline. It also considers how, as they do so, these academics achieve a level of ontological congruence that is only possible as their internal biography, the nature of their day-to-day work and the expectations of their employer are able to ‘rub along’ together without creating the conditions for (self) destructive resistance or the exercise of coercive institutional power.
Copy Number Analysis in a Large Cohort Suggestive of Inborn Errors of Immunity Indicates a Wide Spectrum of Relevant Chromosomal Losses and Gains
Authors: Wan Rensheng , Schieck Maximilian , Caballero-Oteyza Andrés , Hofmann Winfried , Cochino Alexis Virgil , Shcherbina Anna , Sherkat Roya , Wache-Mainier Clarisse , Fernandez Anita , Sultan Marc , Illig Thomas , Grimbacher Bodo , Proietti Michele , Steinemann Doris 
Published: April 2022

AbstractInborn errors of immunity (IEI) are genetically driven disorders. With the advancement of sequencing technologies, a rapidly increasing number of gene defects has been identified, thereby mirroring the high heterogeneity in immunological and clinical presentations observed in patients. However, for a large majority of patients, no causative single nucleotide variant (SNV) or small indel can be identified using next-generation sequencing. First studies have shown that also copy number variants (CNVs) can cause IEI. Unfortunately, CNVs are not well examined in many routine diagnostic settings and the aim of this study was to assess the number of clinically relevant chromosomal losses and gains in a large cohort. We identified a total of 20 CNVs using whole exome sequencing data of a cohort of 191 patients with a suspected IEI. A definite molecular diagnosis could be made in five patients (2.6%), including pathogenic deletions affecting ICOS, TNFAIP3, and 22q11.2. CNVs of uncertain significance were observed in fifteen patients (7.9%), including deletions of 11q22.1q22.3 and 16p11.2 but also duplications affecting entire or parts of genes previously associated with IEI. Importantly, five patients carrying a CNV of uncertain significance also carried pathogenic or likely pathogenic SNVs (PIK3R1, NFKB1, NLRC4, DOCK2), or SNVs of unknown significance (NFKB2). This cooccurrence of SNVs and CNVs suggests modifying effects in some patients, and functional follow-up is warranted now in order to better understand phenotypic heterogeneity. In summary, the diagnostic yield of IEI can be increased substantially by evaluating CNVs, which allows an improved therapeutic management in those patients.
Intravenous Immunoglobulins Promote an Expansion of Monocytic Myeloid-Derived Suppressor Cells (MDSC) in CVID Patients
Authors: Simón-Fuentes Miriam , Sánchez-Ramón Silvia , Fernández-Paredes Lidia , Alonso Bárbara , Guevara-Hoyer Kissy , Vega Miguel A. , Corbí Angel L. , Domínguez-Soto Ángeles 
Published: April 2022

AbstractCommon variable immunodeficiency disorders (CVID), the most common primary immune deficiency, includes heterogeneous syndromes characterized by hypogammaglobulinemia and impaired antibody responses. CVID patients frequently suffer from recurrent infections and inflammatory conditions. Currently, immunoglobulin replacement therapy (IgRT) is the first-line treatment to prevent infections and aminorate immune alterations in CVID patients. Intravenous Immunoglobulin (IVIg), a preparation of highly purified poly-specific IgG, is used for treatment of immunodeficiencies as well as for autoimmune and inflammatory disorders, as IVIg exerts immunoregulatory and anti-inflammatory actions on innate and adaptive immune cells. To determine the mechanism of action of IVIg in CVID in vivo, we determined the effect of IVIg infusion on the transcriptome of peripheral blood mononuclear cells from CVID patients, and found that peripheral blood monocytes are primary targets of IVIg in vivo, and that IVIg triggers the acquisition of an anti-inflammatory gene profile in human monocytes. Moreover, IVIg altered the relative proportions of peripheral blood monocyte subsets and enhanced the proportion of CD14+ cells with a transcriptional, phenotypic, and functional profile that resembles that of monocytic myeloid-derived suppressor cells (MDSC). Therefore, our results indicate that CD14 + MDSC-like cells might contribute to the immunoregulatory effects of IVIg in CVID and other inflammatory disorders.
Selectivity analysis of 99Tc determination by LSC in the field of nuclear decommissioning
Authors: Rozas Saroa , López Julen , Idoeta Raquel , Herranz Margarita 
Published: April 2022

AbstractIn this work, two Tc radiochemical isolation methods—a conventional method by Triskem TEVA® resin and a rapid method by Empore™ Tc Rad Disk—are compared in the field of nuclear decommissioning and 99Tc assessment. The conventional method results more selective than the rapid one, being able to remove almost 100% of the main radiological interferers with the exception of the 90Y; however, the rapid method obtains higher chemical yields (97% vs. 80%) and slightly lower detection limits (0.025 Bq vs. 0.030 Bq) than the conventional. Both methods are similar with regards to equipment and reagent costs.
The evolutionary argument against naturalism: a Wittgensteinian response
Authors: DeVito Michael , McNabb Tyler 
Published: April 2022

AbstractIn this essay, we put forth a novel solution to Plantinga’s Evolutionary Argument Against Naturalism, utilizing recent work done by Duncan Pritchard on radical skepticism. Key to the success of Plantinga’s argument is the doubting of the reliability of one’s cognitive faculties. We argue (viz. Pritchard and Wittgenstein) that the reliability of one’s cognitive faculties constitutes a hinge commitment, thus is exempt from rational evaluation. In turn, the naturalist who endorses hinge epistemology can deny the key premise in Plantinga’s argument and avoid the dilemma posed on belief in the conjunction of naturalism and evolution.
Participatory Action Research, Social Networks, and Gender Influence Soil Fertility Management in Tanzania
Authors: Mponela Powell , Manda Julius , Kinyua Michael , Kihara Job 
Published: April 2022

AbstractTransformation of knowledge systems and fostering learning among smallholder farmers such as through participatory action research (PAR) is key to agricultural growth in rural sub-Saharan Africa. We investigate how PAR influences uptake/use of integrated soil fertility management (ISFM) while accounting for gendered, bonding and bridging social capital. Stratified by engagement in a mother-baby PAR and by resource endowments, 607 smallholder farmers were sampled from northern Tanzania. Binary logistic and multinomial logit models revealed that full engagement in PAR was associated with early adoption of inorganic fertilizers, either as a dichotomous decision or an ISFM bundle with improved varieties, organic matter inputs and soil and water conservation. Bonding social capital through cooperatives, farmer groups, and farmer-farmer local networks supports soil and water conservation, especially among resource-poor farmers. Among the high-resource farmers, increased women’s bargaining power in farm input purchases supports fertilizer and manure use while increased bargaining power in livestock tending supports crop residue incorporation. ISFM usage is constrained by age of decision-makers and a higher number of dependents per worker while education level and farm sizes increase its likelihood. In the resource-constrained, with low extension and technical support, and men-dominated patrilineal farming systems of Africa, the study places PAR, social networks, and gender inclusivity as key approaches for improving smallholder’ ISFM.
Situated self-awareness in expert performance: a situated normativity account of riken no ken
Authors: Miyahara Katsunori , Segundo-Ortin Miguel 
Published: April 2022

AbstractWe explore the nature of expert minds in skilled performance by examining classic Japanese dramatist Zeami’s account of skilled expertise in Noh drama. Zeami characterizes expert minds by the co-existence ofmushinandriken no ken.Mushin(“no-mind”) is an empty state of mind devoid of mental contents.Riken no ken(“seeing with a separate seeing”) is a distinctive form of self-awareness, where the actor embodies a common perspective with the audience upon one’s own performance. Conventional accounts ofriken no kenpresent it as a form of imagination: expert actors deliver their performance by imagining what it looks like from an external point of view. These imagination-based accounts, however, do not square well with the claim thatriken no kenco-exists withmushin. We propose an alternative perception-based account that better accounts for this co-existence, drawing on the concept of “situated normativity” from embodied-ecological theories of cognition. The situated normativity account characterizesriken no kenas a form of “direct affective perception” in which actors are aware of their performance’s quality of attunement with the performative situation. Expert Noh actors realise a common perspective with the audience not by imagining an external point of view, but by perceiving the situation that encompasses their own performance from an aesthetic perspective cultivated and shared within the Noh community.
Innovative Risk Assessment Framework for Hydraulic Control of Irrigation Reservoirs´ Breaching
Authors: Espejo Fernando , Molina José-Luis , Zazo Santiago , Muñoz-Sánchez Rubén 
Published: April 2022

AbstractThis research introduces an innovative framework aimed at developing a risk assessment to analyse the breaching hydraulic control of non-impounding reservoirs for irrigation purposes, called irrigation reservoirs (IRs). This approach comprises an analytical method based on several empirical formulas where the one that best fits the different geometric characteristics of IR water systems is chosen. Furthermore, a stochastic framework allows for the incorporation of the occurrence probability as a tool to characterize the risk analysis of IRs. This occurrence probability has two components: probability based on the bottom elevation of a final breach and probability based on the failure mode (piping in this case). In risk assessment terms, the ultimate product comprises the maximum hazard probability maps that allow a significant improvement in the representation of the artificial flooding effect. This research was successfully applied in two dimensions, synthetically and realistically, in the Las Porteras and Macías Picavea IR water systems (Spain). This approach may improve the management of this type of hydraulic infrastructure and its surrounding area by reducing the risk of experiencing negative consequences derived from uncontrolled hydraulic breaching.
Behavior and interactions of the plant growth-promoting bacteria Azospirillum oryzae NBT506 and Bacillus velezensis UTB96 in a co-culture system
Authors: Bagheri Negar , Ahmadzadeh Masoud , Mariotte Pierre , Jouzani Gholamreza Salehi 
Published: April 2022

AbstractThe objective of the present study was to evaluate possible interactions between two potential plant growth-promoting bacteria (PGPB): Azospirillum oryzae strain NBT506 and Bacillus velezensis strain UTB96. To do this, the growth kinetic, biofilm formation, motility, surfactin production, indole-3-acetic acid (IAA) production, phosphate solubilization and enzyme activities of the strains were measured in monoculture and co-culture. The maximum biomass production for the strains in monoculture and co-culture was about 1011 CFU/ml, confirming that these two strains have the potential to grow in co-culture without reduction of biomass efficiency. The co-culture system showed more stable biofilm formation until the end of day 3. Azospirillum showed the maximum IAA production (41.5 mg/l) in a monoculture compared to other treatments. Surfactin promoted both swimming and swarming motility in all treatments. The Bacillus strain in the monoculture and co-culture showed high phosphate solubilizing capability, which increased continuously in the co-culture system after 6 days. The strains showed protease, amylase and cellulase activities in both monoculture and co-culture forms. Chitinase and lipase activities were observed in both the monoculture of the Bacillus strain and the co-culture. Overall, our findings highlight the promotion of biological and beneficial effects of these bacteria when growing together in co-culture.
Synergistic activity and molecular modelling of fosfomycin combinations with some antibiotics against multidrug resistant Helicobacter pylori
Authors: Abouwarda Ahmed Megahed , Ismail Tarek Abdelmonem , Abu El-Wafa Wael Mohamed , Faraag Ahmed Hassan Ibrahim 
Published: April 2022

AbstractAntibiotic resistance represents the main challenge of Helicobacter pylori infection worldwide. This study investigates the potential bactericidal effects of fosfomycin combinations with clarithromycin, metronidazole, ciprofloxacin, amoxicillin, rifampicin, and doxycycline against thirty-six H. pylori strains using the checkerboard and time-kill assay methods. The results showed that ≥ 50% of the strains were resistant to the six antibiotics. Remarkably, only six strains exerted resistance to these antibiotics, with the minimum inhibitory concentrations (MICs) ranges of (3.2–12.8 mg/l), (32–256 mg/l), (3.2–51.2 mg/l), (3.2–25.6 mg/l), (1.6–3.2 mg/l), and (25.6 > 51.2 mg/l), respectively. The seven antibiotics were evaluated through in silico studies for their permeability and ability to bind UDP-N-acetylglucosamine1-carboxyvinyltransferase (MurA) of H. pylori. The results indicated that fosfomycin exhibited the highest predicted membrane permeability (membrane ∆G insert = − 37.54 kcal/mol) and binding affinity (docking score = − 5.310 kcal/mol) for H. pylori MurA, compared to other tested antibiotics. The combinations of fosfomycin with these antibiotics exerted synergistic interactions (Fractional inhibitory concentration, FIC index < 1) against the six strains. Importantly, the combinations of fosfomycin with clarithromycin, doxycycline and rifampicin achieved bactericidal effects (reduction ≥ 3.0 Log10 cfu/ml) against the most resistant H. pylori strain. Notably, these effects increased with presence of metronidazole, which enhanced the activity of the fosfomycin combination with amoxicillin from a weak inhibition to bactericidal effect. This study provides evidence that the combination of fosfomycin with either clarithromycin, amoxicillin, doxycycline, or rifampicin (especially with the presence of metronidazole) could be a promising option for treating MDR H. pylori infection.
Role of inflammatory markers in the diagnosis of vascular contributions to cognitive impairment and dementia: a systematic review and meta-analysis
Authors: Custodero Carlo , Ciavarella Alessandro , Panza Francesco , Gnocchi Davide , Lenato Gennaro M. , Lee Juhan , Mazzocca Antonio , Sabbà Carlo , Solfrizzi Vincenzo 
Published: April 2022

Abstract
Vascular contribution to cognitive impairment and dementia (VCID) is a clinical label encompassing a wide range of cognitive disorders progressing from mild to major vascular cognitive impairment (VCI), which is also defined as vascular dementia (VaD). VaD diagnosis is mainly based on clinical and imaging findings. Earlier biomarkers are needed to identify subjects at risk to develop mild VCI and VaD. In the present meta-analysis, we comprehensively evaluated the role of inflammatory biomarkers in differential diagnosis between VaD and Alzheimer’s disease (AD), and assessed their prognostic value on predicting VaD incidence. We collected literature until January 31, 2021, assessing three inflammatory markers [interleukin(IL)-6, C-reactive protein (CRP), tumor necrosis factor (TNF)-α] from blood or cerebrospinal fluid (CSF) samples. Thirteen cross-sectional and seven prospective studies were included. Blood IL-6 levels were cross-sectionally significantly higher in people with VaD compared to AD patients (SMD: 0.40, 95% CI: 0.18 to 0.62) with low heterogeneity (I2: 41%, p = 0.13). Higher IL-6 levels were also associated to higher risk of incident VaD (relative risk: 1.28, 95% CI: 1.03 to 1.59, I2: 0%). IL-6 in CSF was significantly higher in people with VaD compared to healthy subjects (SMD: 0.77, 95% CI: 0.17 to 1.37, I2: 70%), and not compared to AD patients, but due to limited evidence and high inconsistency across studies, we could not draw definite conclusion. Higher blood IL-6 levels might represent a useful biomarker able to differentiate people with VaD from those with AD and might be correlated with higher risk of future VaD.
Examining weekly heart rate variability changes: a comparison between monitoring methods
Authors: Ruiz-Alias Santiago A. , Marcos-Blanco Aitor , Clavero-Jimeno Antonio , García-Pinillos Felipe 
Published: April 2022

AbstractMonitoring heart rate variability has been commonly performed by different devices which differ in their methods (i.e., night recording vs.
upon awakening measure, pulse vs. R waves, and software signal processing), Thus, the purpose of this study was to determine the level of agreement between different methods of heart rate variability monitoring, represented in two different systems (i.e., the Polar Nightly Recharge™ function present in Polar sport watches and the Polar H10 chest strap synchronized with the Kubios app). A group of 11 recreational athletes performed a concurrent training program for eight weeks and heart rate variability was daily monitored through both devices. Very large correlation (r = 0.714) and good reliability (ICC = 0.817) were obtained between devices through the entire training program. The magnitude-based inference method was also applied to determine the likelihood of the change concerning the smallest worthwhile change. From a baseline corresponding to the first two weeks of the training program, the weekly heart rate variability changes of the following six weeks were determined for each participant with each device. Despite the large correlation and good reliability between devices, there was a 60.6% of discordance in the likelihood interpretation of the change for the 66 weeks evaluated, explained by the random errors found. Thus, practitioners should be aware of these differences if their training groups use different devices or if an athlete interchanges them. The different nuances of each device can condition the heart rate variability data variation which could compromise the interpretation of the autonomic nervous system modulation.