bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular basis of mutual benefits between Cucurbit chlorotic yellows virus (CCYV) transmission and imidacloprid resistance in Bemisia tabaci
Authors: Yan Minghui , He Haifang , Zhang Zelong , Zhang Beibei , Zhu Chaoqiang , Yan Weili , Zhao Chenchen , Li Jingjing , Yan Fengming 
Published: August 2022

AbstractIncreased insecticide resistance among insect vectors and arboviruses results in significant economic losses to agricultural production and poses a continuous threat to food safety. To date, many studies have been conducted on how virus-infected host plants indirectly and plant virus directly change the physiological characteristics of insect vectors. However, the relationship between the insecticide resistance of the insect vector and the virulence of the viral pathogen has not received sufficient attention. Here, we investigated the effect of Cucurbit chlorotic yellows virus (CCYV), a plant virus transmitted by whitefly (Bemisia tabaci) in a semi-persistent manner, on the resistance of B. tabaci to the neonicotinoid imidacloprid. We found CCYV enhanced the resistance of B. tabaci to imidacloprid. The CYP6CM1 gene was significantly up-regulated in viruliferous susceptible B. tabaci compared with non-viruliferous B. tabaci after an acquisition access period of 10 d, including its upstream regulators. Silencing the CYP6CM1 gene by RNA interference reduced the ability of B. tabaci to acquire CCYV compared with the control. Additionally, imidacloprid-resistant B. tabaci outperformed sensitive adults in terms of virus transmission ability. These results suggest that CCYV enhances the ability of imidacloprid-sensitive B. tabaci to resist adverse external factors (insecticides). In turn, B. tabaci resistance affects virus transmission. Thus, to a certain extent, the plant virus and insect vector establish a mutually beneficial relationship that facilitates virus transmission.
Suicidal ideations among students attending vocational school in Germany: a cross-sectional study to identify prevalence and associated risk factors
Authors: Lamlé Patricia , Keuch Lea , Pukas Lilith , Reinsch Juliane , Röder Heiko , Clausner Hanna , Watzke Stefan 
Published: August 2022

Abstract
Aim
Even though students attending vocational school are very vulnerable to the development of mental health problems, few studies identify the correlation between suicidal ideations and vocational education. Therefore, this study aimed to identify the prevalence and associated risk factors for suicidal ideations among vocational students in Germany.

Methods
We conducted a cross sectional survey with a total of 555 pupils. The pupils received a standardized questionnaire, assessing sociodemographic data, the use of protective factors, possible risk factors, traits for neuroticism and depressive symptoms. For our study, we excluded item 9 from the Beck depression index (BDI-II) to identify the prevalence of suicidal ideation.

Results
Our results show that the prevalence of suicidal ideation among students attending vocational school is 23.2%. A combination of feeling lonely, male sex, low importance of religion, little time for hobbies and BMI scores outside the norm show the highest risk for suicidal ideation and can be identified as significant predictors.

Conclusion
This cross-sectional study highlights the mental burden vocational students in Germany are confronted with and underlines the necessity of paying special attention to this group of young adults. Furthermore, it emphasizes the necessity of further studies on this topic, especially including larger student populations. The results should be used to decrease the significant loss of life caused by suicidal behavior in young adults.
Overview and introduction to development of non-ergodic earthquake ground-motion models
Authors: Lavrentiadis Grigorios , Abrahamson Norman A. , Nicolas Kuehn M. , Bozorgnia Yousef , Goulet Christine A. , Babič Anže , Macedo Jorge , Dolšek Matjaž , Gregor Nicholas , Kottke Albert R. , Lacour Maxime , Liu Chenying , Meng Xiaofeng , Phung Van-Bang , Sung Chih-Hsuan , Walling Melanie 
Published: August 2022

AbstractThis paper provides an overview and introduction to the development of non-ergodic ground-motion models, GMMs. It is intended for a reader who is familiar with the standard approach for developing ergodic GMMs. It starts with a brief summary of the development of ergodic GMMs and then describes different methods that are used in the development of non-ergodic GMMs with an emphasis on Gaussian process (GP) regression, as that is currently the method preferred by most researchers contributing to this special issue. Non-ergodic modeling requires the definition of locations for the source and site characterizing the systematic source and site effects; the non-ergodic domain is divided into cells for describing the systematic path effects. Modeling the cell-specific anelastic attenuation as a GP, and considerations on constraints for extrapolation of the non-ergodic GMMs are also discussed. An updated unifying notation for non-ergodic GMMs is also presented, which has been adopted by the authors of this issue.
One-Pot Production of 1,5-Pentanediol from Furfural Through Tailored Hydrotalcite-Based Catalysts
Authors: Barranca A. , Gandarias I. , Arias P. L. , Agirrezabal-Telleria I. 
Published: August 2022

AbstractThe one-pot production of a relevant chemical such as 1,5-pentanediol (1,5-PDO) from sustainable sources (furfural) is a key reaction to compete with existing fossil sources. This work provides new evidence on the influence of the starting reagent, the features of layered double hydrotalcite (LDH)-derived catalysts in the form of mixed metal oxides (MMO) and of reaction conditions on the productivity of 1,5-PDO under batch conditions. Unlike reported studies, these results suggest the direct pathway through furfuryl alcohol intermediates, allowing the one-pot production from furfural at lower temperature than analogous systems. Productivity is maximized when Co2+ species partially substitute Mg2+ species in parent LDH, yielding promising pentanediol yields under mild reaction conditions. MMOs containing Co2+ sites show marked differences compared to analogous bivalent metals, which is here attributed to the position in which reaction intermediates such as furfuryl alcohol are adsorbed onto surface specie. This is consistent with characterized surface species by XRD, temperature programmed reduction under H2, and chemisorption experiments using CO or CO2 as probe molecules, indicative of a proper balance between metal and basic sites onto MMOs. The reported data aim to provide new reaction evidence to contribute into the search of sustainable 1,5-PDO sources.
Graphical Abstract
Green Synthesis of Bioactive Hydroxyapatite/Cellulose Composites from Food Industrial Wastes
Authors: Abdelraof Mohamed , Farag Mohammad M. , Al-Rashidy Zainab M. , Ahmed Hanaa Y. A. , El-Saied Houssni , Hasanin Mohamed S. 
Published: August 2022

AbstractThis work aimed at conversion of worthless indurtial wastes to valuable product. Herein, bioactive composites based on bacterial cellulose (BC) and eggshell or eggshell-derived hydroxyapatite (HAp) were prepared by a green method using Gluconacetobacter xylinum bacteria. The effect of addition of eggshell (BC/Eg) and eggshell-derived HAp (BC/HAp-Eg) on the bacterial cellulose yield, biodegradation and biocompatibility was studied. For comparison, HAp derived from chemical precursors was synthesized (BC/HAp-ch). The resultant composites were characterized by XRD, FTIR, and SEM/EDX. Furthermore, the biodegradation and bioactivity were assessed in SBF, and the cell viability was studied against oral normal cells. The results showed that the productivity of BC applied HAp-derived eggshell (1.83 g/L) was higher than that of using (1.37 g/L). Interestingly, the eggshell was converted to Ca3(PO4)2 during incubation in the bacterial culture medium, while Ca3(PO4)2 was formed as a secondary phase when using either eggshell-derived HAp or chemically-derived. The in vitro bioactivity test in SBF showed that all composites were induced the formation of a bone-like apatite layer on their surface with Ca/P ratio, 1.49, 1.35, and 1.41 for BC/Eg, BC/HAp-ch, and BC/HAp-Eg, respectively, near to the ratio in the natural HAp. Finally, the in vitro cell viability test was confirmed good biocompatibility against the composites. However, at high sample concentration (250 µg/mL), BC/HA-Eg showed the higher cell viability (95.2%) than that of BC/Eg (80.5%) and BC/HA-ch (86.2%). In conclusion, eggshell waste could be used directly with bacterial cellulose to produce bioactive composites without the need to convert it to HAp which reduced the cost of production and thus has a higher economic return. Obiviously, eggshell waste can act as calcium, organic matter source, pH preservation, nuterilizing agent along with potential instead of costly buffering agent in the BC culture medium and further for increased the BC production.
Teachers’ beliefs about assessment and accountability
Authors: Tóth Edit , Csapó Benő 
Published: August 2022

AbstractOne of the main aims of national assessment programmes is to improve the efficacy of education systems; realizing this aim often takes the form of implementing a variety of accountability measures. Using assessment results for accountability purposes is highly controversial, while one of its undesirable impacts is that it generates negative attitudes towards educational assessments among teachers. The aim of this study is to examine lower and upper secondary teachers’ (N = 1552) opinions and beliefs about testing and, more specifically, about the national assessment programme in Hungary. A questionnaire was used to explore teachers’ beliefs about the effects of the assessment system on how they teach, perceived pressure from stakeholders, teachers’ acceptance of assessment programmes and the relationship between these beliefs. Results show that assessment programmes compel teachers to revise their teaching practices — some change to make meaningful gains in student learning, while others turn to practices that are not conducive to a genuine improvement in students’ knowledge, focussing instead on assessment scores. Pressure from inside the school (colleagues and school leaders) and teachers’ attitude towards assessments bring about changes in instruction, such as the reallocation of coaching and improvement in teaching. Sources of pressure outside school (local government and the media) have an indirect effect on changes in teaching because their pressure influences in-school motivators. Pressure from parents and students is felt directly by teachers, but only in limited areas. The results demonstrate that a national assessment programme has a more significant impact on teaching in lower than in upper secondary schools.
Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa
Authors: Nasrollahi Vida , Yuan Ze-Chun , Lu Qing Shi Mimmie , McDowell Tim , Kohalmi Susanne E. , Hannoufa Abdelali 
Published: August 2022

Abstract
Key message
Our results show that SPL12 plays a crucial role in regulating nodule development in Medicago sativa L. (alfalfa), and that AGL6 is targeted and downregulated by SPL12.
Root architecture in plants is critical because of its role in controlling nutrient cycling, water use efficiency and response to biotic and abiotic stress factors. The small RNA, microRNA156 (miR156), is highly conserved in plants, where it functions by silencing a group of SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. We previously showed that transgenic Medicago sativa (alfalfa) plants overexpressing miR156 display increased nodulation, improved nitrogen fixation and enhanced root regenerative capacity during vegetative propagation. In alfalfa, transcripts of eleven SPLs, including SPL12, are targeted for cleavage by miR156. In this study, we characterized the role of SPL12 in root architecture and nodulation by investigating the transcriptomic and phenotypic changes associated with altered transcript levels of SPL12, and by determining SPL12 regulatory targets using SPL12-silencing and –overexpressing alfalfa plants. Phenotypic analyses showed that silencing of SPL12 in alfalfa caused an increase in root regeneration, nodulation, and nitrogen fixation. In addition, AGL6 which encodes AGAMOUS-like MADS box transcription factor, was identified as being directly targeted for silencing by SPL12, based on Next Generation Sequencing-mediated transcriptome analysis and chromatin immunoprecipitation assays. Taken together, our results suggest that SPL12 and AGL6 form a genetic module that regulates root development and nodulation in alfalfa.
Health-related quality of life of children born very preterm: a multinational European cohort study
Authors: Kim Sung Wook , Andronis Lazaros , Seppänen Anna-Veera , Aubert Adrien M. , Barros Henrique , Draper Elizabeth S. , Sentenac Mariane , Zeitlin Jennifer , Petrou Stavros ,   On Behalf of the SHIPS Research Group, Lebeer J. , Van Reempts P. , Bruneel E. , Cloet E. , Oostra A. , Ortibus E. , Sarrechia I. , Boerch K. , Pedersen P. , Toome L. , Varendi H. , Männamaa M. , Ancel P. Y. , Burguet A. , Jarreau P. H. , Pierrat V. , Truffert P. , Maier R. F. , Zemlin M. , Misselwitz B. , Wohlers L. , Cuttini M. , Croci I. , Carnielli V. , Ancora G. , Faldella G. , Ferrari F. , van Heijst A. , Koopman-Esseboom C. , Gadzinowski J. , Mazela J. , Montgomery A. , Pikuła T. , Barros H. , Costa R. , Rodrigues C. , Aden U. , Draper E. S. , Fenton A. , Johnson S. J. , Mader S. , Thiele N. , Pfeil J. M. , Petrou S. , Kim S. W. , Andronis L. , Zeitlin J. , Aubert A. M. , Bonnet C. , Rafei R. El , Seppänen A. V. 
Published: August 2022

Abstract
Purpose
This study aims to (1) describe the health-related quality of life (HRQoL) outcomes experienced by children born very preterm (28–31 weeks’ gestation) and extremely preterm (< 28 weeks’ gestation) at five years of age and (2) explore the mediation effects of bronchopulmonary dysplasia (BPD) and severe non-respiratory neonatal morbidity on those outcomes.

Methods
This investigation was based on data for 3687 children born at < 32 weeks’ gestation that contributed to the EPICE and SHIPS studies conducted in 19 regions across 11 European countries. Descriptive statistics and multi-level ordinary linear squares (OLS) regression were used to explore the association between perinatal and sociodemographic characteristics and PedsQL™ GCS scores. A mediation analysis that applied generalised structural equation modelling explored the association between potential mediators and PedsQL™ GCS scores.

Results
The multi-level OLS regression (fully adjusted model) revealed that birth at < 26 weeks’ gestation, BPD status and experience of severe non-respiratory morbidity were associated with mean decrements in the total PedsQL™ GCS score of 0.35, 3.71 and 5.87, respectively. The mediation analysis revealed that the indirect effects of BPD and severe non-respiratory morbidity on the total PedsQL™ GCS score translated into decrements of 1.73 and 17.56, respectively, at < 26 weeks’ gestation; 0.99 and 10.95, respectively, at 26–27 weeks’ gestation; and 0.34 and 4.80, respectively, at 28–29 weeks’ gestation (referent: birth at 30–31 weeks’ gestation).

Conclusion
The findings suggest that HRQoL is particularly impaired by extremely preterm birth and the concomitant complications of preterm birth such as BPD and severe non-respiratory morbidity.
Brain, cognitive, and physical disability correlates of decreased quality of life in patients with Huntington’s disease
Authors: Junca Estefanía , Pino Mariana , Santamaría-García Hernando , Baez Sandra 
Published: August 2022

Abstract
Purpose
Following a case–control design, as a primary objective, this study aimed to explore the relationship between quality of life (QoL) scores and gray matter (GM) volumes in patients with Huntington’s disease (HD). As a secondary objective, we assessed the relationship between QoL scores and other important behavioral, clinical and demographical variables in patients with HD and HD patients’ caregivers.

Methods
We recruited 75 participants (25 HD patients, 25 caregivers, and 25 controls) and assessed their QoL using the World Health Organization Quality of Life scale-Brief Version (WHOQOL-BREF). Participants were also assessed with general cognitive functioning tests and clinical scales. In addition, we acquired MRI scans from all participants.

Results
Our results showed that patients exhibited significantly lower scores in all four QoL domains (physical health, psychological wellbeing, social relationships, and relationship with the environment) compared to caregivers and controls. Caregivers showed lower scores than controls in the physical health and the environmental domains. In HD patients, lower scores in QoL domains were associated with lower GM volumes, mainly in the precuneus and the cerebellum. Moreover, in HD patients, physical disability and GM volume reduction were significant predictors of QoL decrease in all domains. For caregivers, years of formal education was the most important predictor of QoL.

Conclusions
HD patients exhibit greater GM volume loss as well as lower QoL scores compared to caregivers and controls. However, caregivers displayed lower scores in QoL scores than controls, with years of education being a significant predictor. Our results reflect a first attempt to investigate the relationships among QoL, GM volumes, and other important factors in an HD and HD caregiver sample.
Modulation of osteoblast differentiation and function by the P2X4 receptor
Authors: Orriss Isabel R. , Davies Bethan K. , Bourne Lucie E. , Arnett Timothy R. 
Published: August 2022

Abstract
Bone cells are known to express multiple P2 receptor subtypes, and the functional effects of receptor activation have been described for many of these. One exception is the P2X4 receptor, which despite strong expression in osteoblasts and osteoclasts, has no defined functional activity. This study used the selective P2X4 receptor antagonists, 5-BDBD and PSB-12062, to investigate the role of this receptor in bone. Both antagonists (≥ 0.1 μM) dose-dependently decreased bone formation by 60–100%. This was accompanied by a ≤ 70% decrease in alkaline phosphatase activity, a ≤ 40% reduction in cell number, and a ≤ 80% increase in the number of adipocytes present in the culture. The analysis of gene expression showed that levels of osteoblast marker genes (e.g. Alpl, Bglap) were decreased in 5-BDBD treated cells. Conversely, expression of the adipogenic transcription factor PPARG was increased 10-fold. In osteoclasts, high doses of both antagonists were associated with a reduction in osteoclast formation and resorptive activity by ≤ 95% and ≤ 90%, respectively. Taken together, these data suggest that the P2X4 receptor plays a role in modulating bone cell function. In particular, it appears to influence osteoblast differentiation favouring the osteogenic lineage over the adipogenic lineage.
The running performance of elite ladies Gaelic football with respect to position and halves of play
Authors: Malone Shane , McGuinness Aideen , Duggan John David , Murphy Amy , Collins Kieran , O’Connor Cliodhna 
Published: August 2022

Abstract
Purpose
The current investigation aimed to examine the running performance of elite Ladies Gaelic football (LGF) match-play and establish the within game positional profile, in addition to the running performance of players across halves of play.

Methods
GPS technology was used to examine the running performance of thirty-three (n = 33) elite LGF players (age; 23 ± 5 years, height; 173 ± 5 cm, body mass; 63 ± 4 kg). Across the duration of the observational period, one hundred and thirty-one (n = 131) individual samples were collected over 15 competitive matches. Data were classified based on positional line and across halves of play. Running performance was determined across the following performance variables of total distance covered (m) (TD), relative distance (m·min−1), HSR (≥ 4.4 m·s−1), RHSR (HSR; m·min−1), percentage HSR (% HSR), VHSR (≥ 5.5 m·s−1), peak velocity (m s−1), percentage peak velocity (% PeakV), accelerations (n; ≥ 3 m·s−2) and decelerations (n; ≤ − 3 m·s−2),

Results
Independent of position the mean distance covered during match play was 7319 ± 1021 m which equates to a relative work rate of 116 ± 9 m·min−1. The high-speed distance of players was 1547 ± 432 m, which equates to a relative high-speed running distance of 25 ± 11 m·min−1. The maximal velocity of players was 7.17 ± 0.41 m·s−1 reflective of a relative maximal velocity of 86 ± 4 percent. The greatest distances were covered by half-backs, midfielders, and half-forwards, with these positions covering significantly greater distances than full-backs (p ≤ 0.05; ES: 0.59–1.25; small-moderate) and full-forwards (p ≤ 0.05; ES: 0.44–1.21; small-moderate). While TD and maximal velocity was shown not to change across halves of play, significant reductions (p ≤ 0.05) were observed across HSR (ES: 0.64–1.02; moderate), accelerations and decelerations (ES: 0.59–1.20; moderate).

Conclusion
The current investigation is the first of its kind within elite LGF, the data within the investigation can aid the coaching process by allowing for the development of sport-specific training regimen specific to the positional needs of elite LGF players.
Temporal trend of diarrhea morbidity rate with climate change: Egypt as a case study
Authors: Saad-Hussein Amal , Helmy Mona Adel , Ellaithy Lamia Samir , Wheida Ali , El Nazer Mostafa , Alfaro Stephane C. , Siour Guillaume , Borbon Agnes , Abdel Wahab Mohamed Magdy , Mostafa Amira N. 
Published: August 2022

AbstractMany studies have detected a relationship between diarrhea morbidity rates with the changes in precipitation, temperature, floods, droughts, water shortage, etc. But, most of the authors were cautious in their studies, because of the lack of empirical climate-health data and there were large uncertainties in the future projections. The study aimed to refine the link between the morbidity rates of diarrhea in some Egyptian governorates representative of the three Egyptian geographical divisions with the meteorological changes that occurred in the 2006–2016 period for which the medical data are available, as a case study. Medical raw data was collected from the Information Centre Department of the Egyptian Ministry of Health and Population. The meteorological data of temperature and precipitation extremes were defined as data outside the 10th–90th percentile range of values of the period of study, and their analysis was done using a methodology similar to the one recommended by the WMO and integrated in the CLIMDEX software. Relationships between the morbidity rates of diarrhea in seven Egyptian governorates and the meteorological changes that occurred in the period 2006 to 2016 were analyzed using multiple linear regression analysis to identify the most effective meteorological factor that affects the trend of morbidity rate of diarrhea in each governorate. Statistical analysis revealed that some meteorological parameters can be used as predictors for morbidity rates of diarrhea in Cairo, Alexandria, and Gharbia, but not in Aswan, Behaira, and Dakahlia where the temporal evolution cannot be related with meteorology. In Red Sea, there was no temporal trend and no significant relationships between the diarrhea morbidity rate and meteorological parameters. The predictor meteorological parameters for morbidity rates of diarrhea were found to be depending on the geographic locations and infrastructures in these governorates. It was concluded that the meteorological data that can be used as predictors for the morbidity rate of diarrhea is depending on the geographical location and infrastructures of the target location. The socioeconomic levels as well as the infrastructures in the governorate must be considered confounders in future studies.