bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract LB056: The spatial landscape of progression and immunoediting in primary melanoma at single cell resolution
Authors: Nirmal Ajit Johnson , Maliga Zoltan , Vallius Tuulia , Quattrochi Brian , Chen Alyce , Jacobson Connor , Pelletier Roxanne , Yapp Clarence , Arias-Camison Raquel , Chen Yu-An , Lian Christine , Murphy George F. , Santagata Sandro , Sorger Peter K. 
Published: June 2022

Abstract
Cutaneous melanoma is a highly immunogenic malignancy, surgically curable at early stages, but life-threatening when metastatic. The spatial organization of the tumor ecosystem during early-stage melanoma is not well understood. Here we integrate high-plex imaging, 3D high-resolution microscopy, and spatially-resolved micro-region transcriptomics to study immune evasion and immunoediting in primary melanoma. We collected highly multiplexed single-cell data from 70 distinct histological regions from 13 specimens (patients) selected to have multiple progression-associated histologies within a single resection. These histologies range from pre-malignant fields in which melanocytic atypia represents the first steps in cancer initiation to non-invasive (radial growth phase) and invasive (vertical growth phase) primary melanoma that eventually gives rise to disseminated disease. We find that recurrent cellular neighborhoods involving tumor, immune, and stromal cells change significantly along a progression axis involving precursor states, melanoma in situ, and primary invasive tumor. Hallmarks of immunosuppression were detectable as early as the melanoma precursor stage, and when tumors become locally invasive, a consolidated and spatially restricted environment with multiple overlapping immunosuppressive mechanisms forms along the tumor-stromal boundary. This environment is established by cytokine gradients that promote expression of MHC-II and IDO1 and by PDL1-expressing macrophages and dendritic cells engaging activated T cells. However, only a few millimeters away, T cells synapse with melanoma cells in fields of tumor regression. Thus, invasion and immunoediting can co-exist within a few millimeters of each other in a single specimen. Multiplexed single-cell imaging and micro-region mRNA profiling link morphological and molecular features of tumor evolution within and across primary cancer specimens, revealing highly localized programs of immune and tumor cell communication via paracrine cytokine signaling and direct cell-cell contact.
Citation Format: Ajit Johnson Nirmal, Zoltan Maliga, Tuulia Vallius, Brian Quattrochi, Alyce Chen, Connor Jacobson, Roxanne Pelletier, Clarence Yapp, Raquel Arias-Camison, Yu-An Chen, Christine Lian, George F. Murphy, Sandro Santagata, Peter K. Sorger. The spatial landscape of progression and immunoediting in primary melanoma at single cell resolution [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr LB056.
Abstract LB126: Functional limitations in respiratory complex I underlie mitochondrial bioenergetics in human colorectal adenocarcinoma
Authors: Boykov Ilya , Montgomery McLane M. , Hagen James T. , Coalson Hannah S. , Fisher-Wellman Kelsey H. 
Published: June 2022

Abstract
Typified by oxidative phosphorylation (OXPHOS), mitochondria catalyze a wide variety of cellular processes now understood to be critical for malignant growth. As such, there is considerable interest in targeting mitochondrial OXPHOS in cancer. The present project was based on the premise that the development of efficacious mitochondrial-targeted anti-cancer compounds requires answering two fundamental questions: 1) is mitochondrial bioenergetics in fact different between cancer and non-cancer cells? and 2) If so, what are the underlying mechanisms? To address these questions, we developed a methodological workflow designed to integrate in vitro functional readouts of mitochondrial flux with mass-spectrometry-based proteomics. This allowed us to directly quantitate both total respiratory capacity and OXPHOS kinetics on a per mitochondrion basis. Experiments were performed in isolated mitochondria prepared from freshly resected human colorectal adenocarcinomas (COAD). Relative to adjacent normal colonic mucosa, tumor mitochondria consistently presented with partial disruptions in respiratory complex I (CI). Surprisingly, CI functional limitations did not impair absolute oxidative flux. Instead, the tumor mitochondrial network displayed clear evidence of intrinsic remodeling. Such remodeling maintained respiratory competence at the expense of decreased oxidative ATP synthesis (i.e., OXPHOS inefficiency or increased ‘uncoupling’). Given recent findings demonstrating that mitochondrial OXPHOS is dispensable for tumor growth, our data raise the intriguing possibility that the requirement for mitochondria in COAD may have little to do with oxidative ATP production, but instead reflect a requirement for accelerated mitochondrial flux to support other aspects of anabolic growth (e.g., extracellular nutrient uptake, NAD+ regeneration, nucleotide synthesis). In this respect, rather than functioning as energy producers, COAD mitochondria may primarily function as ‘energy sinks’.
Citation Format: Ilya Boykov, McLane M. Montgomery, James T. Hagen, Hannah S. Coalson, Kelsey H. Fisher-Wellman. Functional limitations in respiratory complex I underlie mitochondrial bioenergetics in human colorectal adenocarcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr LB126.
Abstract LB198: Cellular selectivity of STING stimulation determines priming of anti-tumor T cell responses
Authors: Jneid Bakhos , Bochnakian Aurore , Delisle Fabien , Djacoto Emeline , Denizeau Jordan , Sedlik Christine , Fiore Frédéric , Kramer Robert , Walters Ian , Carlioz Sylvain , Malissen Bernard , Piaggio Eliane , Manel Nicolas 
Published: June 2022

Abstract
T cells that recognize tumor antigens are crucial for anti-tumor immune responses. Induction of anti-tumor T cells in immunogenic tumors depends on STING, the intracellular innate immune receptor for cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) and related cyclic dinucleotides (CDN). However, the optimal way to leverage STING activation in non-immunogenic tumors is still unclear. Here, we show that cGAMP delivery by intra-tumoral injection of virus-like particles (cGAMP-VLP) leads to differentiation of tumor-specific T cells, decrease in tumor regulatory T cells (Tregs) and anti-tumoral responses that synergize with PD1 blockade. By contrast, intra-tumoral injection of synthetic CDN leads to tumor necrosis and systemic T cell activation but no differentiation of tumor-specific T cells, and a demise of immune cells in injected tumors. Analyses of cytokine responses and genetic models revealed that cGAMP-VLP preferentially targets STING in dendritic cells at a 1000-fold less dose than synthetic CDN. Sub-cutaneous administration of cGAMP-VLP showed synergy when combined with a tumor Treg-depleting antibody to elicit systemic tumor-specific T cells, leading to complete and lasting tumor eradication. These findings show that cell targeting of STING stimulation shapes the anti-tumor T cell response and reveal a therapeutic strategy with T cell modulators, which may address the current limitations of STING-based approaches in patients.
Citation Format: Bakhos Jneid, Aurore Bochnakian, Fabien Delisle, Emeline Djacoto, Jordan Denizeau, Christine Sedlik, Frédéric Fiore, Robert Kramer, Ian Walters, Sylvain Carlioz, Bernard Malissen, Eliane Piaggio, Nicolas Manel. Cellular selectivity of STING stimulation determines priming of anti-tumor T cell responses [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr LB198.
Abstract LB206: Characterizing the PK/PD relationship of C/EBPβ antagonist peptide ST101 in a mouse orthotopic breast cancer model
Authors: Rotolo Jim , Koester Mark , Ramirez Rick , Gallagher Erin , Ghamsari Lila , Leong Siok , Darvishi Emad , Gonzales Jerel , Taylor Timothy , Upton Richard , Merutka Gene , Kappel Barry 
Published: June 2022

Abstract
The transcription factor CCAAT/Enhancer Binding Protein β (C/EBPβ) is aberrantly activated in many cancers, where it drives a genetic profile that promotes survival and proliferation of tumor cells and inhibits differentiation. ST101 is a peptide antagonist of C/EBPβ that prevents its dimerization by binding the leucine zipper domain, ultimately targeting C/EBPβ for proteosomal degradation. Here we describe the pharmacokinetic/pharmacodynamic (PK/PD) profile of ST101 in a non-clinical orthotopic breast cancer model. An integrated population PK/PD model was developed to describe the exposure−response relationship for ST101 anti-tumor activity in female Balb/c mice bearing 4T1-luc triple-negative breast cancer orthotopic tumors. Exposure−response predictions were made based on estimated potency in mice and mean plasma concentrations observed at the doses investigated. Approximately 100% tumor growth inhibition (TGI) was observed at the highest doses administered in this study. The AUC values required to achieve IC50 and IC90 were 80,500 and 99,600 ng/ml*hr, respectively, corresponding to cumulative doses of 97 mg/kg and 120 mg/kg per week in mice. The integrated model provides a useful tool to characterize and predict the ST101 dose-response relationship, provide a quantitative rationale for dose selection in a phase 2 clinical trial and supports the continued development of ST101 as a potent peptide therapeutic for patients with solid tumors.
Citation Format: Jim Rotolo, Mark Koester, Rick Ramirez, Erin Gallagher, Lila Ghamsari, Siok Leong, Emad Darvishi, Jerel Gonzales, Timothy Taylor, Richard Upton, Gene Merutka, Barry Kappel. Characterizing the PK/PD relationship of C/EBPβ antagonist peptide ST101 in a mouse orthotopic breast cancer model [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr LB206.
Pardo, algumas reflexões preliminares sobre a produção das identidades e as formas de identificação dos diferentes sujeitos, no Paraná (1853/1888)
Authors: Oliveira Ricardo de 
Published: June 2022

A proposta do artigo é discutir criticamente a construção histórica da categoria de classificação racial denominada pardo. O texto analisa dois contextos históricos: o presente nacional e a situação social daqueles identificados ou autoidentificados como pardos; o século XIX, a condição do pardo e sua relação com o trabalho escravo., bem como sua representação em jornais da época, especificamente na província do Paraná. O objetivo é refletir sobre a contrução de identidades racializadas, no passado e no presente, e como essas identidades operam dentro de uma hierarquia racista como um dos pilares do sistema capitalista. Palavras-chave: Pardo, identidade, escravidão.
Contribuições do novo constitucionalismo latino-americano para o debate do Marco Temporal para a demarcação de terras indígenas no Brasil
Authors: Pitre Paloma Gerzeli , Silva Andrea Rosendo da 
Published: June 2022

A discussão sobre o marco temporal para a demarcação de terras indígenas está atualmente aguardando votação no Supremo Tribunal Federal (STF). O tema envolve, dentre outras questões, a discussão sobre a relação entre comunidades indígenas e a natureza. Identificam-se duas abordagens contrapostas pelas quais se pode vislumbrar a questão: uma centrada na ideia de natureza como algo a ser utilizado, usufruído, se alinhando a construção colonial e a outra (dos indígenas) que tem na terra a conexão com a antepassados, a manutenção da própria cultura e a sobrevivência . O presente estudo expõe este conflito e aprofunda o debate a partir da perspectiva dos Estudos Decoloniais, sobretudo por meio dos temas da colonialidade do poder e do saber (QUIJANO, 2005); (GROSFOGUEL, 2018); (MIGNOLO, 2001). Neste sentido, emprega-se, além da pesquisa bibliográfica, a pesquisa documental para analisar como o novo constitucionalismo latino-americano e, em especial, a Constituição do Equador de 2008 propõe a relação entre seres humanos e a natureza. Espera-se que os resultados possam agregar mais uma perspectiva para a discussão do marco temporal no Brasil.
Perspectiva decolonial e pensadoras latino-americanas: aportes para análise de narrativas audiovisuais na América Latina
Authors: Silva Andrea Rosendo da , Oliveira Dennis de 
Published: June 2022

Os estudos das relações de gênero na perspectiva Decolonial/Anticolonial estão cada vez mais fortalecidos. Observando esse fenômeno, o artigo reflete sobre as contribuições das pensadoras latino-americanas sobre colonialidade do poder, ser, saber e do gênero. Parte-se do questionamento se os estudos de representação e representatividade avançam em relação à discussão sobre outras formas de ver e olhar e, a partir das questões levantadas, pretende-se promover aportes para o campo da cultura visual e estudos de visualidades. Almeja-se com a pesquisa bibliográfica apresentar resultados que possam incentivar estudos mais densos sobre visualidades em sentido oposto às narrativas coloniais, sobretudo em produções audiovisuais realizadas por mulheres amefricanas e ameríndias, como conceitua Lélia Gonzalez.
O olhar e a distância da porta: palavras zapatistas dão os primeiros passos às terras insubmissas
Authors: Muniz Juliana Esquenazi 
Published: June 2022

Entre 2020 e 2021, durante a pandemia do Coronavírus, o Exército Zapatista por Libertação Nacional (EZLN) lança uma série de comunicados no blog Enlace Zapatista sobre a Gira, projeto de volta ao mundo em distintos continentes a começar por Slumil K’ajxemk’Op (termo no idioma tzotzil que significa terra insubmissa, forma como o EZLN passou a chamar a Europa). Tendo como eixo central a análise do comunicado “O olhar e a distância da porta”, publicado em outubro de 2020, este artigo procura refletir sobre como o imaginário zapatista expresso no texto atua no que o movimento chama de sua 'guerra contra o esquecimento’ e como os exercícios de prefiguração propostos no comunicado estão a favor de um gesto que busca ampliar a resistência anticapitalista, operando contra a colonialidade. Gostaria de sugerir que, se epístolas já foram instrumentos para tentar justificar processos como a colonização europeia, o comunicado zapatista oferece em um momento tão crítico a nível global um movimento oposto, que extrapola fronteiras em nome da luta por ‘um mundo onde caibam todos os mundos’.
Desafios interseccionais compartilhados: categorias de opressão e encontros formativos transdisciplinares em escolas paulistanas
Authors: Calado Maria da Glória 
Published: June 2022

Este trabalho visa apresentar o percurso inicial do processo de formação de professores com foco antirracista e para a igualdade de gênero no contexto pandêmico. Parte-se da pesquisa de campo com a Escola Municipal Levy de Azevedo Sodré, no Campo Limpo, São Paulo, que teve início ainda antes da pandemia, contexto que trouxe implicações para todos os atores escolares e afetou professores e estudantes em aspectos pedagógicos, sociais, econômicos e psicológicos. Nesse âmbito, o objetivo tem sido promover espaços construídos com os professores na realização das docências compartilhadas na perspectiva de uma educação antirracista e emancipatória. Esperamos que o trabalho possa contribuir para o entendimento das opressões intersecionadas e suas possíveis implicações no fazer pedagógico.
Palavras-chave: docências compartilhadas; discriminação; formação de professores; interseccionalidade; racismo.
Inovações no Programa Nacional de Alimentação Escolar: desafios para a gestão no Município de São Paulo
Authors: Mineto Victória Bassan 
Published: June 2022

Este trabalho está vinculado ao campo de estudos da Agricultura Familiar, Segurança Alimentar e Nutricional com enfoque nas Compras Públicas e Mercados Institucionais. O objeto e tema do estudo é o Programa Nacional de Alimentação Escolar (PNAE), programa que oferece alimentação saudável, educação alimentar e nutricional para alunos da educação básica pública. O estudo centra-se na utilização de metodologia essencialmente qualitativa, envolvendo levantamento bibliográfico, análise documental e entrevistas semiestruturadas, tendo como foco investigar a implementação do PNAE no município de São Paulo pelo Programa de Alimentação Escolar (PAE). Dentre os resultados, observou-se que existem dificuldades para alcançar o mínimo de 30% nas compras da agricultura familiar para a alimentação escolar, que envolvem tanto a administração pública como os produtores familiares, sendo necessárias novas estratégias e relações entre os atores.
Distributed Neural Systems Support Flexible Attention Updating during Category Learning
Authors: Weichart Emily R. , Evans Daniel G. , Galdo Matthew , Bahg Giwon , Turner Brandon M. 
Published: June 2022

Abstract
To accurately categorize items, humans learn to selectively attend to the stimulus dimensions that are most relevant to the task. Models of category learning describe how attention changes across trials as labeled stimuli are progressively observed. The Adaptive Attention Representation Model (AARM), for example, provides an account in which categorization decisions are based on the perceptual similarity of a new stimulus to stored exemplars, and dimension-wise attention is updated on every trial in the direction of a feedback-based error gradient. As such, attention modulation as described by AARM requires interactions among processes of orienting, visual perception, memory retrieval, prediction error, and goal maintenance to facilitate learning. The current study explored the neural bases of attention mechanisms using quantitative predictions from AARM to analyze behavioral and fMRI data collected while participants learned novel categories. Generalized linear model analyses revealed patterns of BOLD activation in the parietal cortex (orienting), visual cortex (perception), medial temporal lobe (memory retrieval), basal ganglia (prediction error), and pFC (goal maintenance) that covaried with the magnitude of model-predicted attentional tuning. Results are consistent with AARM's specification of attention modulation as a dynamic property of distributed cognitive systems.
Mid-level Feature Differences Support Early Animacy and Object Size Distinctions: Evidence from Electroencephalography Decoding
Authors: Wang Ruosi , Janini Daniel , Konkle Talia 
Published: June 2022

Abstract
Responses to visually presented objects along the cortical surface of the human brain have a large-scale organization reflecting the broad categorical divisions of animacy and object size. Emerging evidence indicates that this topographical organization is supported by differences between objects in mid-level perceptual features. With regard to the timing of neural responses, images of objects quickly evoke neural responses with decodable information about animacy and object size, but are mid-level features sufficient to evoke these rapid neural responses? Or is slower iterative neural processing required to untangle information about animacy and object size from mid-level features, requiring hundreds of milliseconds more processing time? To answer this question, we used EEG to measure human neural responses to images of objects and their texform counterparts—unrecognizable images that preserve some mid-level feature information about texture and coarse form. We found that texform images evoked neural responses with early decodable information about both animacy and real-world size, as early as responses evoked by original images. Furthermore, successful cross-decoding indicates that both texform and original images evoke information about animacy and size through a common underlying neural basis. Broadly, these results indicate that the visual system contains a mid-level feature bank carrying linearly decodable information on animacy and size, which can be rapidly activated without requiring explicit recognition or protracted temporal processing.
Uncertainty Estimation and Reduction of Pre-trained Models for Text Regression
Authors: Wang Yuxia , Beck Daniel , Baldwin Timothy , Verspoor Karin 
Published: June 2022

AbstractState-of-the-art classification and regression models are often not well calibrated, and cannot reliably provide uncertainty estimates, limiting their utility in safety-critical applications such as clinical decision-making. While recent work has focused on calibration of classifiers, there is almost no work in NLP on calibration in a regression setting. In this paper, we quantify the calibration of pre- trained language models for text regression, both intrinsically and extrinsically. We further apply uncertainty estimates to augment training data in low-resource domains. Our experiments on three regression tasks in both self-training and active-learning settings show that uncertainty estimation can be used to increase overall performance and enhance model generalization.
A Novel Hidden Stitching Method for Preventing Complications in Laparoscopic Hernia Repair
Authors: Wang Liming , Maejima Taku , Fukahori Susumu 
Published: June 2022

Purpose Barbed suture provides a rapid and effective method for closure in minimally invasive surgery. However, postoperative complications involving barbed suture have been reported in recent years. The aim of this study is to introduce a novel hidden stitching technique for peritoneal closure in laparoscopic hernia repair. Method This study retrospectively analyzed the data of patients with laparoscopic transabdominal preperitoneal patch (TAPP). In the hidden stitch (HS) group, the barbed suture was hidden on the dorsal side of the peritoneum and two stitches were returned in the opposite direction after the suture reached the end point. In the non-hidden stitch (NHS) group, the barbed sutured was exposed in the peritoneal cavity with a tail stump of approximately 10 mm preserved to prevent the peritoneal sutures from loosening. Results Twenty-seven patients in the HS group were compared with 53 in the NHS group. There were no differences in age, body mass index, surgical bleeding, or length of hospital stay between the two groups. The peritoneal defect closure time was slightly longer (3 min) in the HS group, but the overall operation time was not significantly extended. There were 8 cases of postoperative complications (P = .035) including 4 cases of bowel obstruction due to the tail of the barbed thread penetrating the small intestine mesenteric and two cases of seroma. There were no postoperative complications in the HS group. Conclusions The hidden stitch method is a safe and feasible peritoneal closure technique that may reduce postoperative complications caused by barbed suture in laparoscopic hernia repair.
“COVID-19, I Hate You!”: Framing Death and Dying in COVID-19 Online Memorials
Authors: Myers Faith , Donley Sarah 
Published: June 2022

The COVID-19 pandemic has reshaped the way Americans deal with death and dying. Social distancing restrictions prevented or delayed end of life services for families who lost loved ones to COVID-19. Alternatively, some took to the internet to express their grief through online memorialization. Using content analysis, this study analyzes 200 randomly selected online memorials from the COVID Memorial website, a website created to share the stories of those lost to COVID-19 on social media. Our findings offer insight into memorial author demographics, how memorial authors framed and understood death due to COVID-19, and the COVID-19 pandemic through online memorialization. Two major themes emerged during data analysis, “references to COVID-19 death” and “COVID-specific directives to readers.” The former, details how memorial authors framed and understood the death of a loved one to COVID-19. The latter details “advice” or “directives” authors gave to readers about social distancing guidelines and other guidelines meant to prevent the spread of COVID-19.