bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypercholesterolemia promotes autoantibody production and a lupus‐like pathology via decreased DNase‐mediated clearance of DNA
Authors: Dhawan Umesh Kumar , Margraf Andreas , Lech Maciej , Subramanian Manikandan 
Published: September 2022

AbstractHypercholesterolemia exacerbates autoimmune response and accelerates the progression of several autoimmune disorders, but the mechanistic basis is not well understood. We recently demonstrated that hypercholesterolemia is associated with increased serum extracellular DNA levels secondary to a defect in DNase‐mediated clearance of DNA. In this study, we tested whether the impaired DNase response plays a causal role in enhancing anti‐nuclear antibody levels and renal immune complex deposition in an Apoe−/− mouse model of hypercholesterolemia. We demonstrate that hypercholesterolemic mice have enhanced anti‐ds‐DNA and anti‐nucleosome antibody levels which is associated with increased immune complex deposition in the renal glomerulus. Importantly, treatment with DNase1 led to a decrease in both the autoantibody levels as well as renal pathology. Additionally, we show that humans with hypercholesterolemia have decreased systemic DNase activity and increased anti‐nuclear antibodies. In this context, our data suggest that recombinant DNase1 may be an attractive therapeutic strategy to lower autoimmune response and disease progression in patients with autoimmune disorders associated with concomitant hypercholesterolemia.
Compiling forty years of guppy research to investigate the factors contributing to (non)parallel evolution
Authors: Heckley Alexis M. , Pearce Allegra E. , Gotanda Kiyoko M. , Hendry Andrew P. , Oke Krista B. 
Published: September 2022

AbstractExamples of parallel evolution have been crucial for our understanding of adaptation via natural selection. However, strong parallelism is not always observed even in seemingly similar environments where natural selection is expected to favour similar phenotypes. Leveraging this variation in parallelism within well‐researched study systems can provide insight into the factors that contribute to variation in adaptive responses. Here we analyse the results of 36 studies reporting 446 average trait values in Trinidadian guppies, Poecilia reticulata, from different predation regimes. We examine how the extent of predator‐driven phenotypic parallelism is influenced by six factors: sex, trait type, rearing environment, ecological complexity, evolutionary history, and time since colonization. Analyses show that parallel evolution in guppies is highly variable and weak on average, with only 24.7% of the variation among populations being explained by predation regime. Levels of parallelism appeared to be especially weak for colour traits, and parallelism decreased with increasing complexity of evolutionary history (i.e., when estimates of parallelism from populations within a single drainage were compared to estimates of parallelism from populations pooled between two major drainages). Suggestive – but not significant – trends that warrant further research include interactions between the sexes and different trait categories. Quantifying and accounting for these and other sources of variation among evolutionary ‘replicates’ can be leveraged to better understand the extent to which seemingly similar environments drive parallel and nonparallel aspects of phenotypic divergence.
Value‐adding in biosecurity surveillance and monitoring: Testing colour and non‐target semiochemical lures on Psylloidea and Pentatomoidea
Authors: Moir Melinda L. , Croeser Louise , Telfer Don , Fenner Christopher , McCauley Rosalie 
Published: September 2022

AbstractCost efficiency in biosecurity surveillance is vital, and the ability to survey multiple pest species using just one trap is, therefore, highly appealing. The Psylloidea, or plantlice, contain significant horticultural pest species that act as vectors for a number of deleterious plant bacteria. We examine the efficacy of using two different coloured sticky traps, and two semiochemical lures on the general Psylloidea and Pentatomoidea fauna, and a target extant pest psyllid; tomato potato psyllid (TPP) Bactericera cockerelli (Šulc) in Western Australia. Specifically, we test the effect of semiochemical lure (no lure, Asian citrus psyllid ACP lure, brown marmorated stink bug BMSB lure, combined lures), sticky trap colour (yellow‐green vs yellow) and sentinel plant (tomato vs citrus) on psyllid and pentatomid species in 104 urban backyards across Perth, Australia. We found that tomato sentinel host plants and yellow‐green traps significantly increased the capture rate of TPP, but that lures decreased the capture of TPP constraining their use. Yellow‐green traps also increased the capture rate of all other Psylloidea. Although BMSB lures reduced TPP capture, these lures increased the abundances of other Psylloidea and the pest stinkbug Plautia affinis (Dallas) on traps. Thus, our experiment demonstrates that increased efficiencies can be gained with multi‐species traps and semiochemical lures for particular groups, provided that they have been tested on focal organisms in the first instance, as reactions to non‐target lures are unpredictable and species specific.
The effect of bait air broadcasting by unmanned aerial vehicles on the ant community diversity
Authors: Li Shenlei , Cui Can 
Published: September 2022

AbstractTo explore the control effect of bait broadcasting by unmanned aerial vehicles (UAVs) on the imported fire ant Solenopsis invicta and evaluate its impact on biodiversity, we tested the drop flow rate and broadcasting evenness of fire ant baits with bait exit hatch sizes, as well as the dynamics of fire ants and other ants when different amounts of bait were used. The amount of bait applied per hectare was 6000–15,000 g in orchards. Fourteen days after broadcasting, the control effect of broadcasting 12,000 g and 15,000 g bait per hectare on active mounds and workers reached 90%, which was significantly higher than that achieved with 6000 g and 9000 g. After the application of bait, the number and species of other ants decreased and then increased; this was reflected in changes in the Shannon–Wiener index (H′), Pielou evenness index (E) and Simpson dominance index (C). The results of this study suggest that broadcasting bait by UAVs can achieve good control of S. invicta, and a reduction in native ant diversity can be achieved in the short term.
Using otolith and body shape to discriminate between stocks of European anchovy (Engraulidae: Engraulis encrasicolus) from the Aegean, Marmara and Black Seas
Authors: Dürrani Ömerhan , Bal Habib , Battal Zeynep Selva , Seyhan Kadir 
Published: September 2022

AbstractEuropean anchovy, a small pelagic fish, plays a significant role in the blue economy, with remarkable commercial, ecological and culinary values. In this study, the variability in the shapes of the body and sagitta otoliths was examined to identify the different anchovy stocks in the Black Sea (26.5°E–39.9°E, 38.7° N–42.1°N) and adjacent regions, i.e., the Sea of Marmara and the Aegean Sea. The body shape was assessed with geometric morphometrics, while shape indices and elliptic Fourier analysis were used to evaluate the shape of the sagitta otoliths. The data were analysed using multivariate and univariate analysis of variance, discriminant function and principal component analysis. The anchovy population could be divided into five distinct stocks based on body shape, with an overall leave‐one‐out cross‐validated correct classification of 85.6%. The geometric morphometrics revealed significant differences in body depth, snout, lower jaw and caudal fin. The differences in sagitta otolith shape also revealed the existence of four distinct stocks with an overall leave‐one‐out cross‐validated correct classification of 46.5%–69.3%. The elliptic Fourier analysis revealed two main stocks of anchovy from the Black Sea as the Eastern–Middle Black Sea stock and the Western Black Sea stock. Based on the elliptic Fourier analysis, the differences in the sagitta otoliths increase in magnitude with increasing geographic separation, first manifested at the otolith anterior notch, followed by changes in the otolith width. The findings affirmed the existence of distinct stocks that should have important implications for effective management of this pelagic fish in the Black Sea and adjacent regions.
Exploring the role of the long short‐term memory model in improving multi‐step ahead reservoir inflow forecasting
Authors: Luo Xinran , Liu Pan , Dong Qianjin , Zhang Yanjun , Xie Kang , Han Dongyang 
Published: September 2022

AbstractDaily inflow forecasting is of vital importance in reservoir economic operation. In the context of hydrometeorological forecasting, the effectiveness of the data‐driven models has been demonstrated as bias correctors for physically‐based models or direct forecasting models. However, existing studies only highlight the performance improvements provided by the data‐driven model, lacking a comprehensive investigation on whether the data‐driven model should be used as bias correctors or direct forecasting models. This study constructs long short‐term memory (LSTM)‐based preprocessing and postprocessing techniques for a hydrological model, which are tested by linear scaling preprocessing and autoregressive (AR) postprocessing models. The integrated model is compared with the LSTM‐only model. The Shuibuya and Zuojiang reservoirs in China are selected as case studies. Results indicate that: (1) LSTM‐based bias correctors are effective in both preprocessing and postprocessing and (2) the integrated model is comparable to the LSTM‐only model when trained with four or more years of data, while it is better than the LSTM‐only model when trained with less data. These findings demonstrate that data‐driven methods can effectively correct the bias in physically‐based model output, and integrating the physical and data‐driven models is useful in improving multi‐step ahead reservoir inflow forecasting if limited data can be obtained.
Sustained activation of the renin–angiotensin–aldosteron system after fetal exposure to AT1 blockers: Effects on kidney and bone in a preterm newborn
Authors: D'Amato Gabriele , Rembouskos Georgios , Cafagna Rosa , Dentico Domenico , Palladino Stefano , Chiarito Mariangela , Faienza Maria Felicia 
Published: September 2022

AbstractThe renin–angiotensin–aldosterone system (RAAS) plays a key role in development of fetal kidney. Angiotensin‐converting enzyme (ACE) inhibitors or angiotensin II receptor type 1 (AT1) antagonists alter RAAS‐signaling compromising metanephrogenesis, and vascular and tubular development. The result is a fetal “RAS blockage syndrome” that may occur not only following exposure during the second and third trimester, but also after the use of these drugs at the beginning of pregnancy. The in‐utero exposure to AT1 antagonists is not confined exclusively to the risk of neonatal renal failure, but also to skull ossification defect that worsens the neonatal prognosis. We report the case of early arterial hypertension development, marked increase of plasma renin and aldosterone, severe hypocalvaria, and low bone mineralization in a female preterm infant in‐utero exposed to AT1 antagonists.
Trends in minimally invasive surgical therapies for overactive bladder management in Australia
Authors: Ng Hung Shin Paul Brian , Rhee Handoo , Chung Eric 
Published: September 2022

AbstractObjectiveTo evaluate the national trends in minimally invasive surgical therapies (MIST) for overactive bladder (OAB) in Australia over the past decade.MethodsAnnual MIST data were extracted using the Australian Medicare Benefit Schedule (MBS) on intravesical botulinum toxin (BTX), sacral nerve modulators (SNM) and percutaneous tibial nerve stimulators (PTNS) performed between 2010 and 2021. Population‐adjusted rates of these procedures were compared in relation to individual states and against the introduction of various OAB drugs during the intervening years.ResultsThe overall national utilization of MIST for OAB has increased over the last decade. The data reflect a rapid uptake in PTNS over the last 2 years following its introduction compared to the relatively steady increase in BTX and SNM over the past decade. There was minimal difference in SNM lead and generator placement, suggesting perhaps the conversion of trial SNM to permanent SNM has been relatively stable across the years. In contrast, there was an increase in PTNS maintenance in the following years following the initial rise in the PTNS treatment initiation. The introduction of various OAB drugs in the market did not seem to significantly affect the pattern of MIST uptake.ConclusionDespite the introduction of various OAB drugs, the overall MIST has increased steadily over the last decade, especially with PTNS. Further exploration into the motivators for specific MIST and cost–benefit analysis of these MIST for OAB is warranted.
Barriers and facilitators to exclusive breastfeeding among Black mothers: A qualitative study utilizing a modified Barrier Analysis approach
Authors: Tran Victoria , Reese Masterson Amelia , Frieson Tomeka , Douglass Frankie , Pérez‐Escamilla Rafael , O'Connor Duffany Kathleen 
Published: September 2022

AbstractBreastfeeding has health benefits for both infants and mothers, yet Black mothers and infants are less likely to receive these benefits. Despite research showing no difference in breastfeeding intentions by race or ethnicity, inequities in breastfeeding rates persist, suggesting that Black mothers face unique barriers to meeting their breastfeeding intentions. The aim of this study is to identify barriers and facilitators that Black women perceive as important determinants of exclusively breastfeeding their children for at least 3 months after birth. Utilizing a Barrier Analysis approach, we conducted six focus group discussions, hearing from Black mothers who exclusively breastfed for 3 months and those who did not. Transcripts were coded starting with a priori parent codes based on theory‐derived determinants mapped onto the Socioecological Model; themes were analysed for differences between groups. Facilitators found to be important specifically for women who exclusively breastfed for 3 months include self‐efficacy, lactation support, appropriate lactation supplies, support of mothers and partners, prior knowledge of breastfeeding, strong intention before birth and perceptions of breastfeeding as money‐saving. Barriers that arose more often among those who did not exclusively breastfeed for 3 months include inaccessible lactation support and supplies, difficulties with pumping, latching issues and perceptions of breastfeeding as time‐consuming. Lack of access to and knowledge of breastfeeding laws and policies, as well as negative cultural norms or stigma, were important barriers across groups. This study supports the use of the Socioecological Model to design multicomponent interventions to increase exclusive breastfeeding outcomes for Black women.
Anti‐inflammatory effects of vagal nerve stimulation with a special attention to intestinal barrier dysfunction
Authors: Bonaz Bruno 
Published: September 2022

AbstractThe vagus nerve (VN), the longest nerve of the organism innervating the gastrointestinal tract, is a mixed nerve with anti‐inflammatory properties through its afferents, activating the hypothalamic–pituitary adrenal axis, and its efferents through the cholinergic anti‐inflammatory pathway inhibiting the release of pro‐inflammatory cytokines (e.g., TNFα) by splenic and gut macrophages. In addition, the VN is also able to modulate the permeability of the intestinal barrier although the VN does not innervate directly the intestinal epithelium. Targeting the VN through VN stimulation (VNS) has been developed in experimental model of intestinal inflammation and in inflammatory bowel disease (IBD) and might be of interest to decrease intestinal permeability in gastrointestinal disorders with intestinal barrier defect such as IBD, irritable bowel syndrome (IBS), and celiac disease. In this issue of neurogastroenterology and motility, Mogilevski et al. report that a brief non‐invasive transcutaneous auricular VNS in healthy volunteers consistently reduces the permeability of the small intestine induced by intravenous administration of the stress peptide corticotropin releasing hormone, known to increase intestinal permeability and to inhibit the VN. In this review, we outline the mechanistic underpinning the effect of stress, of the VN and VNS on intestinal permeability. In particular, the VN can act on intestinal permeability through enteric nerves, and/or cells such as enteric glial cells. We also review the existing evidence of the effects VNS on intestinal permeability in models such as burn intestinal injury and traumatic brain injury, which pave the way for future clinical trials in IBD, IBS, and celiac disease.
Long‐term effects of methamphetamine abuse on visual evoked potentials
Authors: Mahjoob Monireh , Heydarian Samira 
Published: September 2022

AbstractPurposeTo compare visual evoked potential (VEP) components in normal individuals and those with long‐term methamphetamine and crystal methamphetamine use.MethodsIn this study, monocular pattern‐reversal VEPs were recorded in 40 methamphetamine and crystal methamphetamine users and 38 normal individuals. Visual stimuli were high‐contrast (99%) checkerboard patterns at 15 and 60 min of arc with a reversal rate of 1.53 reversals per second.ResultsA significant difference was seen between the two groups for the P100 peak time for the 60 min of arc checks (p = 0.002, d = 0.75, 4.61% higher peak time in the addicted group) and the 15 min of arc checks (p = 0.004, d = 0.73, 4.78% higher peak time in the addicted group). However, other VEP components were not significantly different between the two groups.ConclusionsThe higher P100 peak time at both 15 and 60 min of arc in methamphetamine‐dependent users reveals that VEPs are highly sensitive for the diagnosis of retinal and visual pathway lesions.
Establishment and Evaluation of a Rat Model of Medial Malleolar Fracture with Vascular Injury
Authors: Sun Jinglai , Li Qifeng , Wang Shuo , Wang Guangpu , Zhao Jing , Li Huanming , Liu Chong , Shi Yifan , Li Zhigang , Yu Hui 
Published: September 2022

ObjectiveA stable animal model was needed to study bone non‐union caused by insufficient blood supply, the main object of this paper is to develop a medial malleolar fracture model with controllable arterial vascular injuries in rats for revealing the biochemical mechanism of non‐union by insufficient blood supply.MethodsA total of 18 rats were randomly divided into three equal groups: the Sham group, the Fracture group, and the Fracture + Vascular group. The animals were subjected to unilateral medial malleolar bone fracture and vascular injury using customized molding equipment. The fracture site was scanned by micro‐CT, and vascular injury was evaluated by laser Doppler flowmetry (LDF) 24 h after modeling. Histological examination (HE), alkaline phosphatase (ALP) and tartrate‐resistant acid phosphatase (TRAP) staining, immunohistochemistry and immunofluorescence were conducted on the medial malleolar fracture tissues of three rats randomly selected from each group 24 h after modeling. Subsequently, to further confirm the success of fracture modeling, the fracture sites of three other rats in each group underwent micro‐CT scanning again 6 weeks after surgery.ResultsThe results of a 24 h micro‐CT showed that all rats used to create the fracture models showed controlled injury of the medial malleolus. The model was stable, and the satisfaction of the homemade equipment agreed with the expectation. LDF showed that the blood flow of rats in the Fracture + Vascular group decreased significantly 24 h after fracture injury, while collateral blood flow perfusion increased by 50% on average. The results of HE, ALP and TRAP staining in the medial malleolus showed that the number of osteoblasts (OBs) and osteoclasts (OCs) in the Fracture group increased significantly, but the number of OBs and OCs in the Fracture + Vascular group decreased sharply relative to the number in the Sham group 24 h later. Furthermore, immunohistochemistry and immunofluorescence results showed that the number of neovessels in the Fracture group was significantly increased, while the number of neovessels in the Fracture + Vascular group was significantly decreased, which was consistent with the above results. After 6 weeks of modeling, the micro‐CT results showed that the fractures in the Fracture group had healed substantially, while those in the Fracture + Vascular group had not.ConclusionThis study provided a reproducible and stable experimental animal model for medial malleolar fractures with arterial injury.
Application of a Reflectance Confocal Microscopy Imaging Analysis Score for the Evaluation of Non‐Melanogenic Changes in Male Photoaged Skin
Authors: Infante Victor Hugo , Maia Campos Patricia 
Published: September 2022

AbstractThe photoaging process is characterized by skin changes due to ultraviolet radiation exposure and is the principal environmental factor affecting skin aging. Reflectance confocal microscopy permits noninvasive skin imaging to understand how the photoaging process may change skin. Since men do not habitually use sunscreen, the application of skin imaging techniques is important to understand the influence of sunlight on their skin health. The aim of this study was to develop a score based on RCM imaging analyses to evaluate the morphological and structural changes in the photoaged skin according to literature data. The score was applied in order to determine possible correlations between chronological aging and sunscreen use behavior among men. Thus, 40 men aged 18 to 50 years were recruited, images from the frontal region of their skin were obtained and the score was applied. It was observed that habits are more important than age for the skin photoaging process. Men with photoprotection habits showed overall better skin morphological and structural characteristics regardless of age, demonstrating that sun protection behavior is a major key factor in the understanding of photoaging, so that men should be encouraged to start the use of cosmetic products and perform self‐care.