bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of ATP5G3 in sodium nitroprusside‐induced cell death in cervical carcinoma cells
Authors: Lee Kyung Hye , Rim Do Eun , Lee Jeong‐Hwa , Jeong Seong‐Whan 
Published: December 2022

AbstractWe identified a gene, subunit C3 (ATP5G3) of mitochondrial ATP synthase, that displayed changes in gene expression under oxidative stress. We examined the role of ATP5G3 and its molecular mechanisms in sodium nitroprusside (SNP)‐induced cell death using ATP5G3 small interfering RNA (siATP5G3)‐transfected HeLa cells. A significant increase in cytotoxicity was observed in the transfected cells treated with SNP, which suggests a protective role of ATP5G3 in SNP‐induced cytotoxicity in the cells. The transfected cells treated with photodegraded SNP showed equal cytotoxicity to SNP, and pretreatment with deferoxamine (DFO) completely inhibited this cytotoxicity. Further, cytotoxicity was significantly inhibited by pretreatment with a p38 inhibitor and was accentuated by the p38 activator in cells. Pretreatment with the Bcl‐xL inhibitor also significantly accentuated cytotoxicity. The increase in p38 phosphorylation was significantly higher in siATP5G3‐transfected cells treated with SNP in immunoblotting, which was inhibited by pretreatment with DFO. The increase in cytotoxicity with siATP5G3 transfection was completely blocked by cotransfection with sip38, and the blocking effect disappeared by cotransfection with additional siBcl‐xL, which suggests that the protective role of ATP5G3 is mediated by Bcl‐xL via the inhibition of p38 activity. Cytotoxicity was completely blocked by the cotransfection of siATP5G3 with siBax. No change in apoptotic parameters was observed during cytotoxicity. However, pretreatment with lysosomal inhibitors significantly inhibited cytotoxicity and increased p62 protein levels. These findings suggest that ATP5G3 plays a protective role in autophagic cell death/lysosome‐associated cell death induced by SNP via the sequential signaling of ROS/p38/Bcl‐xL/Bax in HeLa cells.
Hematological characteristics of COVID‐19 patients with fever infected by the Omicron variant in Shanghai: A retrospective cohort study in China
Authors: Wei Tingting , Li Jiangyan , Cheng Zhuo , Jiang Liansheng , Zhang Jiafeng , Wang Hao , Zhou Lin 
Published: December 2022

AbstractBackgroundA wave of the Omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection has rapidly spread in Shanghai, China. Hematological abnormalities have been reported in coronavirus disease 2019 (COVID‐19) patients; however, the difference in hematological parameters between COVID‐19 patients with fever and patients who are febrile from other causes remains unexplored.MethodsThis retrospective cohort study enrolled 663 SARS‐CoV‐2 positive patients identified by RT‐PCR. Clinical parameters, including age, sex, and threshold cycle values of all COVID‐19 patients, and hematological parameters of COVID‐19 patients in the fever clinic were abstracted for analysis.ResultsOverall, 60.8% of COVID‐19 patients were male, and the median age was 45 years. Most of COVID‐19 patients were asymptomatic, while 25.8% of patients showed fever and 10.9% of patients had other emergencies. COVID‐19 patients with fever had significantly lower white blood cells (WBCs), neutrophils, lymphocytes, platelets and C‐reactive protein (CRP), and significantly higher monocyte‐to‐lymphocyte ratio (MLR), platelet‐to‐lymphocyte ratio (PLR), mean platelet volume (MPV), and mean platelet volume‐to‐platelet ratio (MPR) levels, compared with those in SARS‐CoV‐2 negative patients with fever from other causes (p < 0.05). Neutrophil‐to‐lymphocyte ratio (NLR), PLR, and systemic inflammatory index (SII) levels were significantly higher in COVID‐19 patients with emergencies (p < 0.05). WBCs showed the best performance with an area under the curve (0.756), followed by neutrophils (0.730) and lymphocytes (0.694) in the diagnosis of COVID‐19 in the fever clinic.ConclusionWBCs, neutrophils, lymphocytes, platelets, CRP and MLR, PLR, and MPR may be useful in early diagnosis of COVID‐19 in the fever clinic.
m6A‐related long noncoding RNAs predict prognosis and indicate therapeutic response in endometrial carcinoma
Authors: Zhou Xinying , Zhang Hu , Duan Yingchun , Zhu Jianlong , Dai Haiyan 
Published: December 2022

AbstractBackgroundN6‐methyladenosine (m6A) has been identified as the most common, abundant, and conserved internal transcriptional modification. Long noncoding RNAs (lncRNAs) are noncoding RNAs consisting of more than 200 nucleotides, and the expression of various lncRNAs may affect cancer prognosis. The impact of m6A‐associated lncRNAs on uterine corpus endometrial carcinoma (UCEC) prognosis is unknown.MethodsIn this study, UCEC prognosis‐related m6A lncRNAs were screened, bioinformatics analysis was performed, and experimental validation was conducted. Endometrial carcinoma (EC) and normal tissue samples were obtained from The Cancer Genome Atlas. The prognosis‐related m6A lncRNAs screened by the least absolute shrinkage and selection operator method were used for multivariate Cox proportional risk regression modeling. Principal component analysis and Gene Ontology, immune function difference, and drug sensitivity analyses of the prognostic models were performed. Prognostic analysis was conducted for m6A‐associated lncRNAs. The immune infiltration relationship of m6A‐associated lncRNAs in EC was identified using the ssGSEA immune infiltration algorithm. A competing endogenouse RNA network was constructed using the LncACTdb database. Finally, quantitative real‐time polymerase chain reaction (qRT‐PCR) assays were used to validate the differences in m6A‐related lncRNA expression in normal and EC cells.ResultsCDKN2B‐AS1 and MIR924HG were found to be risk factors for EC. RAB11B‐AS1 was a protective factor in EC patients. MIR924HG expression was upregulated in KLE and RL95‐2 endometrial cancer cell lines. Prognostic models involved RAB11B‐AS1, LINC01812, HM13‐IT1, TPM1‐AS, SLC16A1‐AS1, LINC01936, and CDKN2B‐AS1. The high‐risk group was more sensitive to five compounds (ABT.263, ABT.888, AP.24534, ATRA, and AZD.0530) than the low‐risk group.ConclusionThese findings contribute to understanding of the function of m6A‐related lncRNAs in UCEC and provide promising therapeutic strategies for UCEC.
Chairwork and the therapeutic relationship: Can the cart join the horse?
Authors: Pugh Matthew , Dixon Alison , Bell Tobyn 
Published: December 2022

Abstract“Chairwork” is a collection of experiential methods that utilize movement between chairs and dialogue with parts of the self to bring about change. Because of their emotionally intense nature, therapists often assume that a robust therapeutic relationship is a prerequisite for these tasks. However, it could be said that chairwork also supports the development and strengthening of the alliance. This article presents a single‐session, chairwork‐centered treatment with an individual experiencing social anxiety. Verbatim extracts and post‐intervention feedback illustrate the reciprocal and reinforcing roles of client participation, therapist facilitation, and the therapeutic bond during chairwork. Moreover, the case demonstrates that relationship and technique are intimately bound when using experiential methods, suggesting that therapists do not always need to privilege the former to implement the latter.
Body mass index, waist circumference and cardiovascular diseases in transitional ages (40 and 66 years)
Authors: Yoo Jung Eun , Han Kyungdo , Jung Jin‐Hyung , Hur Yang‐Im , Kim Yang Hyun , Kim Eun Sook , Son Jang Won , Rhee Eun‐Jung , Lee Won‐Young , Nam Ga Eun 
Published: December 2022

AbstractBackgroundThere is lack of data on effect modification by age on the association between body mass index (BMI) or waist circumference (WC) and cardiovascular diseases (CVDs). We aimed to investigate the impact of BMI and WC on incident CVDs in individuals aged 40 and 66 years.MethodsOverall, 2 430 510 participants who underwent a national health screening for transitional ages provided by the Korean National Health Insurance Service between 2009 and 2012 were included. The adjusted hazard ratios and 95% confidence intervals for myocardial infarction (MI), ischaemic stroke and CVDs as a composite outcome of MI and ischaemic stroke were calculated using multivariable Cox proportional hazard regression analysis.ResultsDuring a mean follow‐up of 7.7 years, 24 884 MI and 29 415 ischaemic stroke events occurred. Among participants aged 40 years, there was a J‐shaped association of BMI with incident CVDs, MI and ischaemic stroke with nadir at BMI 18.5–22.9 kg/m2 (P for trend < 0.001 for all). Among those aged 66 years, there were significant U‐shaped associations of BMI with CVDs and MI with nadir at a BMI of 23.0–24.9 kg/m2 (P for trend 0.013 and 0.017, respectively). WC was linearly associated with all study outcomes in both age groups (P for trend <&nbsp;0.001). The impact of general and abdominal obesity on both study outcomes was more prominent in those aged 40 years than in those aged 66 years (P for interaction < 0.001).ConclusionsTo prevent cardiovascular risk, weight loss intervention should be cautiously implemented and individualized according to age. The maintenance of muscle mass may be essential in managing weight loss particularly in older population.
An MRI Study Combining Virtual Brain Grafting and Surface‐Based Morphometry Analysis to Investigate Contralateral Alterations in Cortical Morphology in Patients With Diffuse Low‐Grade Glioma
Authors: Zhang Simin , Sun Huaiqiang , Yang Xibiao , Wan Xinyue , Tan QiaoYue , Li Shuang , Shao Hanbin , Su Xiaorui , Yue Qiang , Gong Qiyong 
Published: December 2022

BackgroundThe human brain has ability to reorganize itself in response to glioma. However, the mechanism of cortical reorganization remains unclear.PurposeTo investigate alterations in cortical thickness and local gyration index (LGI) in patients with unilateral frontal lobe diffuse low‐grade glioma (DLGG).Study TypeRetrospective.SubjectsNinety‐nine patients with histopathologically proven DLGG invading the left frontal lobe (LF; N = 56) or the right frontal lobe (RF; N = 43), and healthy controls (HC; N = 53).Field Strength/Sequence3.0 T, 3D T1‐weighted images and gadolinium enhanced T1‐weighted images using magnetization‐prepared rapid gradient echo sequence, T2‐weighted images, and fluid‐attenuated inversion recovery using turbo spin echo sequence.AssessmentIn patients with DLGG, virtual brain grafting combined with Freesurfer was utilized to enable automated cortical thickness and LGI calculation. In HC, standard FreeSurfer pipeline was applied to calculate these measures. Radiomic features were extracted from glioma using Pyradiomic software.Statistical TestsGeneral linear model and Pearson's correlation analysis. A P value <0.05 was considered statistically significant.ResultsFor LF patients, there was significantly increased cortical thickness in the rostral middle frontal gyrus, significantly reduced cortical thickness in the precentral gyrus and hypogyrification in the lingual and medial orbitofrontal (MOF) gyrus in contralateral hemisphere. For RF patients, there was significantly increased cortical thickness in the middle temporal, lateral occipital extending to isthmus cingulate gyrus, significantly reduced cortical thickness in the precentral gyrus and hypogyrification in the lingual gyrus in the contralateral hemisphere. A negative association between four textural features of DLGG and LGI in the right MOF gyrus of LF group was found (r = −0.609, −0.442, −0.545, and −0.417, respectively).Data ConclusionCortical thickness compensation was shown in contralateral homotopic location and some distant contralateral regions. Additionally, there was decreased cortical thickness in the contralateral precentral gyrus and hypogyrification in contralateral lingual gyrus.Evidence Level4Technical EfficacyStage 2
Changes in endemic patterns of respiratory syncytial virus infection in pediatric patients under the pressure of nonpharmaceutical interventions for COVID‐19 in Beijing, China
Authors: Jiang Ming‐Li , Xu Yan‐Peng , Wu Hui , Zhu Ru‐Nan , Sun Yu , Chen Dong‐Mei , Wang Fang , Zhou Yu‐Tong , Guo Qi , Wu Aiping , Qian Yuan , Zhou Hang‐Yu , Zhao Lin‐Qing 
Published: December 2022

AbstractA series of nonpharmaceutical interventions (NPIs) was launched in Beijing, China, on January 24, 2020, to control coronavirus disease 2019. To reveal the roles of NPIs on the respiratory syncytial virus (RSV), respiratory specimens collected from children with acute respiratory tract infection between July 2017 and Dec 2021 in Beijing were screened by capillary electrophoresis‐based multiplex PCR (CEMP) assay. Specimens positive for RSV were subjected to a polymerase chain reaction (PCR) and genotyped by G gene sequencing and phylogenetic analysis using iqtree v1.6.12. The parallel and fixed (paraFix) mutations were analyzed with the R package sitePath. Clinical data were compared using SPSS 22.0 software. Before NPIs launched, each RSV endemic season started from October/November to February/March of the next year in Beijing. After that, the RSV positive rate abruptly dropped from 31.93% in January to 4.39% in February 2020; then, a dormant state with RSV positive rates ≤1% from March to September, a nearly dormant state in October (2.85%) and November (2.98%) and a delayed endemic season in 2020, and abnormal RSV positive rates remaining at approximately 10% in summer until September 2021 were detected. Finally, an endemic RSV season returned in October 2021. There was a game between Subtypes A and B, and RSV‐A replaced RSV‐B in July 2021 to become the dominant subtype. Six RSV‐A and eight RSV‐B paraFix mutations were identified on G. The percentage of severe pneumonia patients decreased to 40.51% after NPIs launched. NPIs launched in Beijing seriously interfered with the endemic season of RSV.
Tracking spatiotemporal evolution of cementitious carbonation via Raman imaging
Authors: Srivastava Sonali , Garg Nishant 
Published: December 2022

AbstractCarbonation of cementitious systems is an important phenomenon from both durability (rebar corrosion) and greenhouse emissions (CO2 sequestration) perspectives. Several analytical techniques are well‐established for measuring the kinetics of this dynamic phenomenon, ranging from measuring the pH change to quantifying the calcite content over time. However, the majority of these methods (with the exception of electron imaging) rely on bulk measurements which may miss the fine, microstructural changes that occur during carbonation. In this study, we investigate and evaluate the potential of Raman imaging to follow the spatiotemporal evolution of carbonation in cementitious pastes. By analysing mm‐scale maps (1 mm × 1 mm) at the micron‐scale resolution, we find that nearly ~40% of the sample surface was covered with calcite at the end of a 2‐week carbonation exposure. Simultaneously, the portlandite content declined from ~15% to 5% in the same period, suggesting portlandite consumption for the sake of calcite growth. Finally, the difference between the calcite growth rate and portlandite consumption rate strongly suggests simultaneous carbonation of CSH and ettringite—a finding that is in agreement with recent experimental investigations of carbonation kinetics. Overall, these new results on the spatiotemporal evolution of cementitious microstructure pave the way for utilizing Raman imaging for understanding dynamic phenomena in complex systems.
The inside‐out surgical anatomy of the paraglottic space a video‐guided endoscopic dissection
Authors: Saleh Hazem Mohamed Aly , Seidel Nadja , Jöns Thomas , Mürbe Dirk , Nawka Tadeus 
Published: December 2022

AbstractObjectivesThe paraglottic space is an essential anatomic compartment of the larynx. It is central to the spread of laryngeal cancer and to the choice of conservative laryngeal surgery and many phonosurgical procedures. Since its description, 60 years ago, the surgical anatomy of the paraglottic space was sparsely revisited. Amid the era of endoscopic and transoral microscopic functional surgery of the larynx, we provide here a long‐awaited description of the inside‐out anatomy of the paraglottic space.MethodologyUsing an endoscope equipped with a 3D camera, we dissected 10 hemilarynges from 5 fresh frozen cadavers from the inside out. Before dissection, we labeled the vessels through injecting them with colored latex. We explored the paraglottic space emphasizing its shape, boundaries, and contents. We documented our findings through endoscopic photography and video recordings.ResultsThe paraglottic space is a spacious tetrahedral space located parallel not only to the glottic, but also to the subglottic and the supraglottic compartments of the laryngeal lumen. It has musculo‐cartilaginous, musculo‐fibrous, and mucosal boundaries. It is separated from the pyriform sinus only by mucosa. A cushion of fat surrounds its vascular and to a lesser extent its neural contents. Harbored intrinsic laryngeal muscles are endoscopically identifiable within the space, namely the thyroarytenoid, the lateral, and posterior cricoarytenoid muscles.ConclusionThe endoscopic description of the paraglottic space partly fills the knowledge gap on the laryngeal anatomy from the inside out. It opens the door for novel diagnostic methods and for ultraconservative functional laryngeal interventions under endoscopic control.Level of EvidenceN/A
Comorbidity associated with worse outcomes in a population of limited cochlear implant performers
Authors: Lee Erika , Pisa Justyn , Hochman Jordan 
Published: December 2022

AbstractIntroductionMost patients significantly benefit from cochlear implantation (CI). However, speech understanding varies widely, with a small proportion of patients demonstrating limited audiometric outcomes. While there are well‐documented determinants of poor performance, there remains a cohort of patients that do not meet expected outcomes. Preoperative prognostication is desirable to manage expectations, ensure value of the intervention, and reduce risk. The objective of the study is to evaluate variables found within a single CI center's most limited functioning cohort following implantation.MethodsA retrospective review of a single CI program's cohort of (344 ears) patients implanted between 2011 and 2018 whose 1‐year postimplantation AzBio scores fall 2 SDs below the mean was performed. Exclusion criteria includes skullbase pathology, pre/peri‐lingual deafness, cochlear anatomic abnormalities, English as an additional language, and limited electrode insertion depth. Overall, 26 patients were identified.ResultsThe study population's postimplantation net benefit AzBio score is 18% compared to the entire program's 47% (p < 0.05). This group is older (71.8 vs. 59.0 years, p < 0.05) with a longer duration of hearing loss (26.4 vs. 18.0 years, p < 0.05) and with a lower preoperative AzBio score [14% lower (p < 0.05)]. A host of medical conditions were identified in the subpopulation, with a trend towards significance in those suffering from either malignancy or cardiac condition. Escalating comorbid status was associated with worse performance (p < 0.05).ConclusionWithin a cohort of limited‐performing CI users, benefit tended to decrease with escalating number of comorbid conditions. This information may serve to inform preoperative patient counseling.Level of evidenceLevel IV (evidence from a case control study).
Evaluating YouTube videos on sialendoscopy as an educational resource for patients
Authors: Campbell Daniel J. , Xiao Kevin , Mastrolonardo Eric , Cognetti David M. 
Published: December 2022

AbstractObjectivesPatients are increasingly relying on YouTube for health information. We objectively evaluated the quality and comprehensiveness of sialendoscopy YouTube videos available to patients. We further investigated the relationship between video content and video popularity.MethodsWe identified 150 videos using the search term “sialendoscopy.” Videos were excluded if they were lectures for medical professionals, operating room (OR) recordings, unrelated, non‐English, or non‐audio. Video quality and comprehensiveness were evaluated using modified DISCERN criterion (range: 5–25) and novel sialendoscopy criterion (NSC, range: 0–7), respectively. Secondary outcomes included standard video metrics and Video Power Index to measure popularity. Videos were classified binarily by uploader type as from an academic medical center or from other sources.ResultsTwenty‐two (14.7%) of 150 videos were included for review, with 7 (31.8%) uploaded from academic medical institutions. One hundred‐nine (72.7%) videos were excluded as lectures for medical professionals or OR recordings. Overall mean modified DISCERN (13.45 ± 3.42) and NSC (3.05 ± 0.96) scores were low; however, videos uploaded by academic medical institutions were significantly more comprehensive (NSC mean difference = 0.98, 95% CI: 0.16–1.80, p = .02). There were no significant correlations between video popularity and objective measures of quality or comprehensiveness.ConclusionsThis study highlights the paucity and low quality of sialendoscopy videos for patients. More popular videos are not higher quality, and most videos are targeted more toward physicians rather than patients. As YouTube becomes increasingly used by patients, there is opportunity for otolaryngologists to produce more informative videos for patients while implementing targeted strategies to increase viewership.Level of EvidenceNA.
Assessing the impacts of anthropogenic sounds on early stages of benthic invertebrates: The “Larvosonic system”
Authors: Olivier Frédéric , Gigot Mathilde , Mathias Delphine , Jezequel Youenn , Meziane Tarik , L'Her Christophe , Chauvaud Laurent , Bonnel Julien 
Published: December 2022

AbstractNoise produced by human activities has increased in the oceans over the last decades. Whereas most studies have focused on the impact of anthropogenic noise on marine mammals and fishes, those focusing on marine invertebrates are rarer and more recent, especially when considering peri‐metamorphic benthic stages, highly sensitive to anthropogenic perturbations. A careful review of the literature reveals a simplistic characterization of the acoustics within the containers used to quantify larval and juvenile responses to noise, thus weakening the conclusions of such works. To address this problem, we developed the Larvosonic system, a laboratory tank equipped with acoustic assets to assess the impacts of noise on young stages of marine invertebrates. We first provide a careful analysis of the tank sound field using different sound types, and we assess the effects of expanded polystyrene units on the sounds emitted by a professional audio system in order to dampen reverberation and resonance. Then, we apply this acoustic calibration to the effects of both pile driving and drilling noises on postlarvae of the scallop bivalve Pecten maximus. Acoustic recordings highlight that diffuser and bass trap components constitute effective underwater sound absorbents, reducing the reflection of the whole frequency bandwidth. Scallop experiments reveal that both type and level of the tested noise influenced postlarval growth, with interactive effects between trophic environment and noise level/spectra. The Larvosonic system thus constitutes an efficient tool for bioacoustics research on bentho‐planktonic invertebrate species.
Nonzero positive solutions of fractional Laplacian systems with functional terms
Authors: Biagi Stefano , Calamai Alessandro , Infante Gennaro 
Published: December 2022

AbstractWe study the existence of nonzero positive solutions of a class of systems of differential equations driven by fractional powers of the Laplacian. Our approach is based on the notion of fixed point index, and allows us to deal with nonlocal functional weights and functional boundary conditions. We present two examples to shed light on the type of functionals and growth conditions that can be considered with our approach.