bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ramipril Improves Endothelial Function and Increases the Number of Endothelial Progenitor Cells in Patients With Systemic Lupus Erythematosus
Authors: Oliveira Ana Cecília Diniz , Arismendi Maria Izabel , Machado Luiz Samuel Gomes , Sato Emilia Inoue 
Published: June 2022

Background/Objectives
Endothelial dysfunction and reduced number of endothelial progenitor cells (EPCs) in peripheral blood are contributing factors to cardiovascular disease in systemic lupus erythematosus (SLE) patients. Endothelial progenitor cell proliferation is regulated by vascular endothelial growth factor (VEGF). Angiotensin-converting enzyme inhibitors reduce cardiovascular mortality in patients with coronary heart disease.

Methods
This was a randomized trial including 37 female SLE patients without cardiovascular risk factors allocated into 2 groups: 19 patients received ramipril 10 mg/d for 12 weeks (IG) and 18 patients maintained without ramipril (CG). Endothelial function was assessed by brachial artery ultrasound measuring flow-mediated dilation, and EPCs were quantified by flow cytometry and cell culture, at baseline and after 12 weeks. Serum VEGF levels were measured by enzyme-linked immunosorbent assay. Statistical analysis was intention to treat. p < 0.05 was considered significant.

Results
After 12 weeks, higher flow-mediated dilation (6.17% vs. 11.14%, p < 0.001) was observed in IG, without change in CG (5.37% vs. 5.02%, p = 0.630). Higher number of EPC colony-forming units was also observed in IG (21.3 ± 10.4 vs. 31.6 ± 8.5, p < 0.001), without difference in CG (p = 0.714). No difference was found in EPCs evaluated by flow cytometry. Vascular endothelial growth factor level increased after 12 weeks in IG (p = 0.048), with no difference in CG (p = 0.661).

Conclusion
Ramipril improved endothelial function and increased the numbers of EPCs evaluated by cell culture and VEGF levels in SLE patients without cardiovascular risk factors. These data suggest that angiotensin-converting enzyme inhibitor bring an extra benefit beyond the hypotensive action and should be considered as a preferred antihypertensive drug in SLE patients.
WAVES (Web-based tool for Analysis and Visualization of Environmental Samples) – a web application for visualization of wastewater pathogen sequencing results
Authors: Triska Petr , Amman Fabian , Endler Lukas , Bergthaler Andreas 
Published: June 2022

SummaryEnvironmental monitoring of pathogens provides an accurate and timely source of information for public health authorities and policymakers. In the last two years, wastewater sequencing proved to be an effective way of detection and quantification of SARS-CoV-2 variants circulating in population. Wastewater sequencing produces substantial amounts of geographical and genomic data. Proper visualization of spatial and temporal patterns in this data is crucial for the assessment of the epidemiological situation and forecasting. Here, we present a web-based dashboard application for visualization and analysis of data obtained from sequencing of environmental samples. The dashboard provides multi-layered visualization of geographical and genomic data. It allows to display frequencies of detected pathogen variants as well as individual mutation frequencies. The features of WAVES for early tracking and detection of novel variants in the wastewater are demonstrated in an example of BA.1 variant and the signature Spike mutation S:E484A. WAVES dashboard is easily customized through the editable configuration file and can be used for different types of pathogens and environmental samples.AvailabilityWAVES source code is freely available at https://github.com/ptriska/WavesDash under MIT license.
Androgen receptor inhibition induces metabolic reprogramming and increased reliance on oxidative mitochondrial metabolism in prostate cancer
Authors: Crowell Preston D. , Giafaglione Jenna M. , Jones Anthony E. , Nunley Nicholas M. , Hashimoto Takao , Delcourt Amelie M.L. , Petcherski Anton , Bernard Matthew J. , Huang Rong Rong , Low Jin-Yih , Matulionis Nedas , Guan Xiangnan , Navone Nora M. , Alumkal Joshi J. , Haffner Michael C. , Ye Huihui , Zoubeidi Amina , Christofk Heather R. , Shirihai Orian S. , Divakaruni Ajit S. , Goldstein Andrew S. 
Published: June 2022

AbstractProstate cancer cells that survive clinical androgen receptor (AR) blockade mediate disease progression and lethality. Reprogrammed metabolic signaling is one mechanism by which tumor cells can survive treatment. However, how AR inhibition reprograms metabolism, and whether altered metabolism can be exploited to eradicate cells that survive AR blockade, remains unclear. Here, we comprehensively characterized the effect of AR blockade on prostate cancer metabolism using transcriptomics, metabolomics, and bioenergetics approaches. AR inhibition maintains oxidative mitochondrial metabolism and reduces glycolytic signaling, through hexokinase II downregulation and decreased MYC activity. Robust elongation of mitochondria via reduced DRP1 activity supports cell fitness after AR blockade. In addition, AR inhibition enhances sensitivity to complex I inhibitors in several models, suggesting that AR blockade increases reliance on oxidative mitochondrial metabolism. Our study provides an enhanced understanding of how AR inhibition alters metabolic signaling and highlights the potential of therapies that target metabolic vulnerabilities in AR-inhibited cells.
ISCA2 inhibition decreases HIF and induces ferroptosis in clear cell renal carcinoma
Authors: Green Yangsook Song , dos Santos Maria C. Ferreira , Fuja Daniel , Reichert Ethan , Campos Alexandre R. , Cowman Sophie J. , Kohan Jessica , Tripp Sheryl R. , Leibold Elizabeth A. , Sirohi Deepika , Agarwal Neeraj , Liu Xiaohui , Koh Mei Yee 
Published: June 2022

AbstractClear cell renal cell carcinoma (ccRCC), the most common form of kidney cancer, is typically initiated by inactivation of the von Hippel Lindau (VHL) gene, which results in the constitutive activation of the hypoxia inducible factors, HIF-1α and HIF-2α. Using a high throughput screen, we identify novel compounds that decrease HIF-1/2α levels and induce ferroptosis by targeting Iron Sulfur Cluster Assembly 2 (ISCA2), a component of the late mitochondrial Iron Sulfur Cluster (L-ISC) assembly complex. ISCA2 inhibition either pharmacologically or using siRNA decreases HIF-2α protein levels by blocking iron-responsive element (IRE)-dependent translation, and at higher concentrations, also decreases HIF-1α translation through unknown mechanisms. ISCA2 inhibition also triggers the iron starvation response, resulting in iron/metals overload and death via ferroptosis. ISCA2 levels are decreased in ccRCC compared to normal kidney, and decreased ISCA2 levels are associated with pVHL loss and with sensitivity to ISCA2 inhibition. Strikingly, pharmacological inhibition of ISCA2 using an orally available ISCA2 inhibitor significantly reduced ccRCC xenograft growth in vivo, decreased HIF-α levels and increased lipid peroxidation, suggesting increased ferroptosis in vivo. Thus, the targeting of ISCA2 may be a promising therapeutic strategy to inhibit HIF-1/2α and to induce ferroptosis in pVHL deficient cells.
The ER protein translocation channel subunit Sbh1 controls virulence ofCryptococcus neoformans
Authors: Santiago-Tirado Felipe H. , Hurtaux Thomas , Geddes-McAlister Jennifer , Nguyen Duy , Helms Volkhard , Doering Tamara L. , Römisch Karin 
Published: June 2022

AbstractThe fungal pathogenCryptococcus neoformansis distinguished by a cell wall-anchored polysaccharide capsule that is critical for virulence. Biogenesis of both cell wall and capsule relies on the secretory pathway. Protein secretion begins with polypeptide translocation across the endoplasmic reticulum (ER) membrane through a highly conserved channel formed by three proteins: Sec61, Sbh1, and Sss1. Sbh1, the most divergent, contains multiple phosphorylation sites, which may allow it to regulate entry into the secretory pathway in a species- and protein-specific manner. Absence ofSBH1causes a cell-wall defect in bothSaccharomyces cerevisiaeandC. neoformans, although other phenotypes differ. Notably, proteomic analysis showed that when cryptococci are grown in conditions that mimic aspects of the mammalian host environment (tissue culture medium, 37 °C, 5% CO2), a set of secretory and transmembrane proteins is upregulated in wild-type, but not inΔsbh1mutant cells. The Sbh1-dependent proteins show specific features of their ER targeting sequences that likely cause them to transit less efficiently into the secretory pathway. Many also act in cell-wall biogenesis, while several are known virulence factors; consistent with these observations, theC. neoformans Δsbh1mutant is avirulent in a mouse infection model. We conclude that, in the context of conditions encountered during infection, Sbh1 controls the entry of virulence factors into the secretory pathway ofC. neoformans, and thereby regulates fungal pathogenicity.ImportanceCryptococcus neoformansis a yeast that causes almost 200,000 deaths worldwide each year, mainly of immunocompromised individuals. The surface structures of this pathogen, a protective cell wall surrounded by a polysaccharide capsule, are made and maintained by proteins that are synthesized inside the cell and travel outwards through the secretory pathway. A protein called Sbh1 is part of the machinery that determines which polypeptides enter this export pathway. We found that when Sbh1 is absent, bothC. neoformansand the model yeastS. cerevisiaeshow cell wall defects. Lack of Sbh1 also changes the pattern of secretion of both transmembrane and soluble proteins, in a manner that depends on characteristics of their sequences. Notably, multiple proteins that are normally upregulated in conditions similar to those encountered during infection, including several needed for cryptococcal virulence, are no longer increased. Sbh1 thereby regulates the ability of this important pathogen to cause disease.
3D (x-y-t) Raman imaging of tomato fruit cuticle: microchemistry during development
Authors: González Moreno Ana , Domínguez Eva , Mayer Konrad , Xiao Nannan , Bock Peter , Heredia Antonio , Gierlinger Notburga 
Published: June 2022

AbstractThe cuticle of tomato fruits was studied in-situ using Confocal Raman Microscopy. Microsections from cuticles isolated at different developmental stages were scanned to reveal the distribution of cuticle components with a spatial resolution of 342 nm by univariate and multivariate data analysis. From the three main components, cutin, polysaccharides and aromatics, the latter one exhibit the strongest Raman scattering intensity. Therefore, Raman imaging opened the view on phenolic acids and flavonoids within the cuticle and resulted in three schematic cuticle models depicting development.At the earliest stage of development, which corresponded to the procuticle layer, phenolic acids were found across the entire cuticle. Based on a mixture analysis with reference component spectra, the phenolic acids were identified as mainly esterified p-coumaric acid together with free p-hydroxybenzoic acid. Later in development, during the cell expansion period of growth, phenolic acids accumulated in an outermost layer of the cuticle and in the middle region of the pegs. In these stages of development cellulose and pectin were appeared towards the epidermal layer, where later during ripening the flavonoid impregnation started. In the first ripening stage chalconaringenin was observed, while methoxylated chalcones were chosen by the algorithm to fit the mature cuticle spectra. The co-location with carbohydrates and esterified p-coumaric acid and methoxylated chalconaringenin suggest that they link polysaccharide and cutin domains. Within the cutin matrix, aromatics confer mechanical and thermal functions, while the outermost phenolic acid layer displays UV-B protection of the plant tissue.One-sentence summaryNew insights into the distribution of cutin, carbohydrates and phenolics along cross sections of green and mature tomato fruit cuticles by Raman mapping and multivariate data analysis.
Interactions in CSF1-driven Tenosynovial Giant Cell Tumors
Authors: van IJzendoorn David G.P. , Matusiak Magdalena , Charville Gregory W. , Spierenburg Geert , Varma Sushama , Colburg Deana R.C. , van de Sande Michiel A.J. , van Langevelde Kirsten , Mohler David G. , Ganjoo Kristen N. , Bui Nam Q. , Avedian Raffi S. , Bovée Judith V.M.G. , Steffner Robert , West Robert B. , van de Rijn Matt 
Published: June 2022

AbstractThe majority of cells in Tenosynovial Giant Cell Tumor (TGCT) are macrophages responding to CSF1 that is overproduced by a small number of neoplastic cells with a chromosomal translocation involving the CSF1 gene. Treatment with inhibitors of the CSF1 pathway has been clinically effective. An autocrine loop was postulated where the neoplastic cells are stimulated through the CSF1 receptor (CSF1R) expressed on their surface. Here we show that the neoplastic cells themselves do not express CSF1R and therefore may be unaffected by current therapies. We identified a new marker for synoviocytes, GFPT2, that highlights the tumor cells in TCGT and is associated with activation of the YAP1/TAZ pathway. The neoplastic cells in TGCT are highly similar non-neoplastic synoviocytes. Finally, we provide molecular support for the osteoclast-like features of the giant cells in TGCT that correlate with the destructive effects of TGCT on bone.
Validation of a Deep Learning Model to aid in COVID-19 Detection from Digital Chest Radiographs
Authors: Ajmera Pranav , Kharat Amit , Khaladkar Sanjay , Gupte Tanveer , Pant Richa , Kulkarni Viraj , Duddalwar Vinay , Patkar Deepak , Bhatia Mona , Lamghare Purnachandra 
Published: June 2022

AbstractIntroductionUsing artificial intelligence in imaging practice helps ensure study list reprioritization, prompt attention to urgent studies, and reduces the reporting turn-around time.PurposeWe tested a deep learning-based artificial intelligence model that can detect COVID-19 pneumonia patterns from digital chest radiographs.Material and MethodsThe deep learning model was built using the enhanced U-Net architecture with Spatial Attention Gate and Xception Encoder. The model was named DxCOVID and was tested on an external clinical dataset. The dataset included 2247 chest radiographs comprising CXRs from 1046 COVID-19 positive patients (positive on RT-PCR) and 1201 COVID-19 negative patients.ResultsWe compared the performance of the model with three different radiologists by adjusting the model’s sensitivity as per the individual radiologist. The area under the curve (AUC) on the receiver operating characteristic (ROC) of the model was 0.87 [95% CI: 0.85, 0.89].ConclusionWhen compared to the performance of three expert readers, DxCOVID matched the output of two of the three readers. Disease-specific deep learning models using current technology are mature enough to match radiologists’ performance and can be a suitable tool to be incorporated into imaging workflows.
Resisting accounting in the name of discipline
Authors: Perray-Redslob Ludivine , Morales Jeremy 
Published: June 2022

PurposeThis paper examines micro-practices of resistance to understand how they influence accounting.Design/methodology/approach A qualitative methodology based on interviews is used to explore an extreme case of disciplinary organization, that of the French Armed Forces whereby secrecy and discipline are the norm. The study draws on James Scott's concept of infrapolitics to illustrate how service members manage to appear obedient and disciplined, while simultaneously criticizing and resisting accounting practices “below the radar” of surveillance.FindingsThe study describes “resistance in obedience” to account for how service members resist while following discipline. Three main forms of resistance are identified. Containment consists in obstructing and delaying a process of change that depends on willing participation of active supporters. Subversion consists in weakening the sources of information and the communication channels. Sabotage consists in fragmenting accounting (here a balanced scorecard) by separating performance indicators from cost accounting. The study shows that these three tactics of hidden and informal resistances prevent the spread of accounting reforms, disrupt transparency and create a blockade around financial information.Research limitations/implicationsThe study of resistance to accounting in a setting where compliance and discipline are the norm shows how widespread it can be. In that respect, future research could provide a more systematic understanding of resistance in action and its conditions of possibility in various contexts and settings. This article further illustrates the allure of opacity against the threats of transparency and accountability. The use of accounting in opaque settings opens interesting avenues of research, since the appeal of accounting has often been related to the allure of transparency and to accounting's potential to create visibilities. Finally, this paper opens a perspective for future research on how micro-resistance meets micro-practices of power in the context of ostensibly liberated, participative and non-authoritarian management.Originality/valueWhile previous literature argued that resistance to accounting arises when it is used to increase discipline, our findings challenge this assumed dichotomy, by showing that sometimes accounting is resisted in the name of discipline. This study further outlines the fact that the “allure” of transparency is not universal but can also prove disruptive and be contested. In addition, this study contributes to the literature on resistance to accounting that mainly focused on overt, dramatic and organized forms of resistances, by highlighting the existence of a more widespread, omnipresent yet hidden and mundane, day-to-day, form of opposition, which significantly influences accounting. Finally, the findings show that resistance is not only an outside force intersecting with accounting but also an intrinsic force that shapes accounting from the inside.
Omni micro-reseller's path-to-purchase and MSEs omnichannel readiness in the Indonesian affordable fashion industry
Authors: Maulana Amalia E. , Adisantoso Julio , Hartanto Bobie 
Published: June 2022

PurposeThis study aims to present the path-to-purchase of omni micro-resellers in affordable fashion shopping centers and differentiates them from the existing knowledge of end-user’s purchase journey. Furthermore, the study aims to explore the omnichannel readiness for Micro and Small Enterprises (MSEs) merchants to provide a seamless experience for the micro-resellers.Design/methodology/approachThis study adopted an ethnographic approach to gain deep insights into the unexplored omni journey of micro-resellers, using multiple techniques and respondent types in various locations.FindingsFindings reveal that the journey of omni micro-reseller is not the same as the end-user's purchase journey. The new value needed in every path-to-purchase is an essential knowledge that helps MSE merchants in serving micro-resellers. MSE merchants' readiness is assessed by their ability to provide the best customer experience for their buyers, consisting of six omnichannel experience dimensions: researching, purchase-payment, shipping, omnichannel testing, return goods experience and relationship building.Research limitations/implicationsUsing the Engel-Kollat-Blackwell (EKB) decision-making model, this study develops the path-to-purchase of omnichannel micro-resellers. The new readiness dimensions developed in this study are set as a potential measurement tool.Practical implicationsIt provides new insights to benefit MSE merchants and the institutions responsible for enhancing merchant quality.Originality/valueThis study focuses on micro-resellers in the MSEs environment, the prominent buyers of affordable fashion in developing countries, which is a novelty of the study. Moreover, unlike previous studies that have focused on large and medium merchants, this study concentrates on MSE merchants. To the best of the authors’ knowledge, this study is one of the first studies to highlight ways to measure MSE merchants omnichannel readiness.
Global scenarios under crises: the case of post COVID-19 era
Authors: Hafezi Reza , Asemi Pardis 
Published: June 2022

Purpose
COVID-19 pandemic has significantly influenced human society; statistics have shown many countries ended 2020 with minimal or even negative economic growth. This pandemic proved that development biased to economic issues without considering other factors such as social and environmental is not sustainable, and it can even be the source of the crisis. This paper aims to discover plausible alternative futures at the macrolevel where nations, businesses and societies can change routines, and approach to a better peaceful future.

Design/methodology/approach
To accomplish this, trends emerged after the COVID-19 pandemic are studied and some uncertainties are identified. At the intersections of uncertainties, scenarios are shaped based on the Global Business Network (GBN) methodology. GBN helps to draw macrolevel images about plausible futures. The main uncertainties rose from three different but interconnected natures including: approach to the globalization (social perspective), approach to the concept of value (business perspective) and approach to the governance (states/governance perspective). Each uncertainty can take two extreme alternatives; consequently, eight scenario spaces with different probability are formed by the intersection of alternatives. Then the most favorable scenario is introduced and strategies are proposed to achieve a better future.

Findings
In the current paradigm in which countries will sacrifice the environment for economic growth in the race to rapidly rebuild their economy, concerns for the post-COVID-19 will continue to mount. While, in the favorable scenario, value creation is sought in sustainable development. This means deviation from a favorable scenario to achieve short-term goals is completely predictable. Although we have learned that the COVID-19 pandemic is controllable and manageable as time goes by, but no effort or commitment has been seen in the governments to eradicate the COVID-19 sources (at least till today).

Originality/value
The main originality of this research appeared in the practical aspect. This paper analyzes and projects scenarios at the global level and studies challenges that societies, governments and businesses are facing in the modern world with biased development paradigms. Moreover, different viewpoints to deal with global crisis are assessed and criticized.