bluuebunny/crossref_metadata_2025_split huggingface dataset:
From risk reduction to a landscape of (un)desired outcomes: Climate migrants' perceptions of migration success and failure
Authors: Tubi Amit , Israeli Yael 
Published: July 2022

Abstract
Migration is increasingly viewed an adaptation strategy. Much of the migration-as-adaptation research applies a climate risk analytical lens, examining to what extent migration is a successful/adaptive response that reduces vulnerability or a failed/maladaptive response that increases vulnerability. However, this body of research has largely failed to examine migration outcomes through the eyes of those who migrate, thereby yielding insights that are potentially disconnected from their realities and desirable futures. To address this gap, this study focuses on the case of drought-influenced migration from agro-pastoralist northern Kenya to the City of Nairobi. Applying a thematic analysis based on semi-structured interviews with 38 long-term migrants, we examine how migrants perceive ‘success’ and ‘failure’ in the context of migration as a response to the impacts of drought. The findings show that migrants’ success perceptions focus on the improvement of their families’ economic security, namely fulfilling their basic needs and reconstructing households’ livelihood sources. Another related objective – supporting children’s education – was often perceived as a means to shift the next generation out of agro-pastoralism, a perception that evolved in light of migrants’ experiences in Nairobi. A similar failure theme pertains to migrants’ inability to support their families. However, another predominant theme emphasizes self-failure, mainly as cultural assimilation in Nairobi. Associated with substance abuse and emotional disconnection from the north, assimilation was perceived as a potential trigger of cascading failures, including migrants’ inability to achieve their adaptation-related objectives. As these findings show, success and failure perceptions, including adaptation-related, are mediated by social-cultural factors and are intertwined with migrants’ broader concerns and aspirations. As such concerns and aspirations may shape migrants’ and households’ vulnerability trajectories, research must shift from framing migration narrowly as a climate risk reduction strategy, to conceptualizing it as a process of navigating a landscape of desired and undesired outcomes.
Separation and analysis of Bacillus subtilis respiratory chain complexes.
Authors: Garrido Gerardo Ignacio Picón , García Ana Paula García , Vara Luis González de la , Chagoya-López Alicia , Gómez-Lojero Carlos , Gutiérrez-Cirlos Emma Berta 
Published: July 2022

Abstract
Bacillus subtilis is a Gram-positive bacterium with a respiratory chain embedded in the cytoplasmic membrane. The respiratory chain is bifurcated after menaquinol into a cytochrome b6c + caa3 branch and a quinol oxidase branch, with up to three quinol oxidases. The complexes that generate the proton gradient are the b6c + caa3 supercomplex, and it is proposed to form respiratory strings in the membrane. The other proton-generator is the quinol oxidase aa3. There is still information missing about the quinol branch and if the aa3 is associated with the electron donor complexes. It is unclear whether succinate quinone reductase (SQR) can form associations with the quinol branch or the cytochrome branch. In this paper, we show the separation of part of the b6c complex associated with cytochromes c550 and c551. We obtained a b6c + caa3 supercomplex of around 600 kDa and SQR, aa3, and NADH dehydrogenase by dodecyl maltoside solubilization and separation of the respiratory chain components by ionic exchange chromatography. We found that aa3 does not associate with other complexes. SQR was associated with the b6c complex in a mutant lacking aa3. This association could facilitate electron transfer from SQR to menaquinone-7. The lack of associations between the abundant quinol oxidase aa3 and other complexes is a feature we are still studying.
Effect of Novosphingobium sp. CuT1 inoculation on the rhizoremediation of heavy metal- and diesel-contaminated soil planted with tall fescue
Authors: Lee Soo Yeon , Lee Yun-Yeong , Cho Kyung-Suk 
Published: July 2022

Abstract
Rhizoremediation is a promising method based on the synergism between plant and rhizobacteria, which constitutes a promising means to remediate soil co-contaminated with heavy metals and total petroleum hydrocarbons (TPHs). A plant growth-promoting (PGP) rhizobacterium with diesel-degrading capacity and heavy metals tolerance was isolated from the rhizosphere of tall fescue (Festuca arundinacea L.), after which the effects of its inoculation on rhizoremediation performance were evaluated in heavy metal and diesel-contaminated soil planted with tall fescue. The bacterial isolate (Novosphingobium sp. CuT1) was characterized by its production of indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and siderophore productivity as PGP traits. CuT1 was able to grow on 1/10 LB-agar plates containing 5 mM of Cu or 5 mM of Pb. Compared to the rhizoremediation performance of the co-contaminated soil (Cu + Pb + diesel) without inoculation, the bioavailable Cu concentrations in soil and the biomass of tall fescue were significantly increased CuT1 inoculation. Additionally, the root growth of tall fescue was also promoted CuT1 inoculation. Correlation analysis showed that Cu bioavailability and bioconcentration factor were positively correlated with CuT1 inoculation. The diesel removal efficiency showed a positive correlation with CuT1 inoculation although diesel removal was below 30%. CuT1 inoculation was positively correlated with IAA and dehydrogenase activity in soil. Moreover, the dry biomass of the tall fescue’s roots was highly associated with CuT1 inoculation. Collectively, our findings suggest that Novosphingobium sp. CuT1 can be utilized as an applicable bioresource to enhance rhizoremediation performance in heavy metal- and TPH-contaminated soils.
Triple facilitation therapy in the non-acute stage of post-stroke dyskinesia: protocol for a single-blind randomized controlled trial
Authors: wang ying , Jin Song , Guo Hong , Xiao Guisheng , Zhang Guilong , Xie Quan , Sun Jianfeng , Wu Zhipeng , Lin Keyu , Wang Qin , Liang Shundong , Zhang Di 
Published: July 2022

Abstract
Background and purpose: Dyskinesia is a series of movement disorders arising from a stroke. Western medicine focuses on anticoagulation, which can lead to intracranial and gastrointestinal bleeding, and reduce the body's immunity. Traditional therapies including acupuncture, tuina, and Chinese herbs, etc., works slowly as well as the relatively simple efficacy. The triple facilitation therapy, an integrated rehabilitation therapy of Traditional Chinese and Western Medicine, consisting of Lee's pestle needle therapy, Du’s massage therapy, and functional exercise, has effects on improving muscle strength, reducing muscle tension, and assisting in establishing normal movement patterns according to a pilot trial. Whether the triple facilitation therapy is superior to Zhongfengxingnao Oral Liquid, a commonly used Chinese patent medicine in dyskinesia patients after stroke remains unclear. This study aims to compare the effect and safety of triple facilitation therapy and Zhongfengxingnao oral liquid in improving motor function and quality of life in post-stroke dyskinesia patients, and to further verify the non-inferiority of the triple facilitation therapy.Methods and analysis: This study is a randomized-controlled, single-blind trial of triple facilitation therapy (n=33) versus Zhongfengxingnao oral liquid (n=33) in the non-acute stage of post-stroke dyskinesia patients. MMT freehand muscle strength grading, modified Ashworth grading, simplified Fugl-Meyer scoring, isokinetic muscle test outcomes, and 3-D gait analysis outcomes are the primary outcomes. The secondary outcomes include ADL/disability (FIM), general indexes, biochemical indexes, and adverse events. All the primary and secondary outcomes will be assessed at baseline (1 day) and 4-weeks/post-intervention (28 days). This study will confirm whether triple facilitation therapy improves rehabilitation of the non-acute stage of post-stroke dyskinesia better than Zhongfengxingnao oral liquid. Ethics and dissemination: Institutional Review Board of the Hospital of Chengdu University of Traditional Chinese Medicine approved the research and will obtain the written informed consent of each subject or representative.Trial registry number: ChiCTR2100045105 (www.chictr.org.cn)
Effect of mPFC KLF4 on autism-like behaviors and possible mechanisms
Authors: Ge Tongtong , Zhao Haisheng , Dong Mengmeng , Yang Wei , Li Bingjin , cui ranji 
Published: July 2022

Abstract
Autism spectrum disorder (ASD) is a series of neurodevelopmental disorders with two core symptoms, repetitive behavioral phenotype, and social impairments, yet the underlying cellular and molecular mechanisms are still obscure. KLF4 (Kruppel-like factor 4) is a zinc finger protein transcription factor that has been widely reported in the regulation of cell proliferation, differentiation, survival, and apoptosis. Recently, increasing evidence demonstrated its critical role in neurodevelopment, microglia activating, and neuroinflammation in the central nervous system (CNS). Little is known about the effect of KLF4 in the medial prefrontal cortex (mPFC) on autism and autism-like behaviors. Here, we found that high and low expression levels of KLF4 in mPFC induced significant social novelty impairments. The molecular mechanisms might be associated with overexpression of KLF4 in mPFC promote activation of microglia and altered synaptic E/I ratio by affecting the expression of GluA1 and GABA-related proteins, the specific molecular mechanisms still need more investigations.
Insights into the Phaeozem Formation from the Fluvial Parent Material
Authors: Guanxin Du , Baiquan Yan , Yu Sun , Fan Yang , Cheng Qian , Tao Qin , Yanqing Zang 
Published: July 2022

Abstract
The formation and evolution of phaeozems have always been an area of interest because the phaeozems play an important role in two regions dubbed as "European Bread Basket" and "Northeast China Storehouse." As the phaeozems contain a large proportion of parent materials and are sensitive to human intervention, their formation and evolution have been restricted. Due to the homogeneity of loess parent material, there has been extensive research on the formation of surface phaeozems from the loess parent material. However, phaeozems developed from key fluvial parent material have so far received little attention. Therefore, key information on the formation of phaeozems is missing. In this context, this study applies geochemical tests to determine rare earth elements and major and trace elements in the deep phaeozem layer from the Arongqi borehole in the phaeozems of Northeastern China. Further, the sporopollen fossil tests were conducted in the HT-1-4-2 borehole, and the distribution characteristics of the deep phaeozem layer were analyzed using geostatistics. The results from this study indicate that rare earth data from phaeozems in Arongqi's deep layer shows a comparatively high total concentration of rare earth elements, with a mean value of 141.45 mg.kg− 1. The chondrite normalization curve demonstrates that the distribution of light rare earth elements shows a positive slope. Further, Eu shows a trend with a slight negative anomaly, while Ce shows a trend with an evident negative anomaly. Ce > -0.1 (mean value 3.9) indicates a reducing environment with hypoxia. The distribution of rare earth elements indicates that phaeozems contain transportable parent materials with trace elements Th/U > 2 (mean value 2.88), Sr/Ba < 0.6 (mean value 0.38), 0 < Rb/Sr < 0.6 (mean value 0.26), Sr /Cu > 10 (mean value 30.98), and climate index at mean value of 0.23. The presence of trace elements indicated freshwater habitats and a dry-hot to semi-dry-hot climate. The sporopollen fossils are mainly comprised of shrubs, coniferous forests, and terrestrial herbs. The presence of Ephedra and Polygonaceae indicates that the environment was hot and arid at that time. Moreover, the presence of several ferns observed in the vertical profile indicates an intermittent warm and humid climate between droughts. Thus, it can be concluded that the deep phaeozems in Arongqi developed in the floodplains of Arun River under the dry-hot-semi-dry-hot climatic conditions.
Gradient-based parameter optimization to determine membrane ionic current composition of human induced pluripotent stem cell-derived cardiomyocytes
Authors: Kohjitani Hirohiko , Koda Shigeya , Himeno Yukiko , Makiyama Takeru , Yamamoto Yuta , Yoshinaga Daisuke , Wuriyanghai Yimin , Kashiwa Asami , Toyoda Futoshi , Zhang Yixin , Amano Akira , Noma Akinori , Kimura Takeshi 
Published: July 2022

Abstract
Premature cardiac myocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) show heterogeneous action potentials (APs), most probably because of different expression patterns of membrane ionic currents. We aim to develop a method of determining expression patterns of functional channels in terms of the whole-cell ionic conductances (Gx) using individual spontaneous AP configurations. However, it has been suggested that apparently identical AP configurations were obtained by different sets of ionic currents in a mathematical model of cardiac membrane excitation. If so, the inverse problem of Gx estimation might not be solved. We computationally tested the feasibility of the gradient-based optimization method. For realistic examination, conventional 'cell-specific models' were prepared by superimposing the model output of AP on each experimental AP record by the conventional manual adjustment of Gxs of the baseline model. Then, Gxs of 4 ~ 6 major ionic currents of the 'cell-specific models' were randomized within a range of ±5 ~ 15% and were used as initial parameter sets for the gradient-based automatic Gxs recovery by decreasing the mean square error (MSE) between the target and model output. When plotted all data points of MSE - Gx relation during the optimization, we found that the randomized population of Gxs progressively converged to the original value of the cell-specific model with decreasing MSE. To confirm the absence of any other local minimum in the global search space, we mapped the MSE by randomizing Gxs over a range of 0.1 ~ 10 times the control. No additional local minimum of MSE was obvious in the whole parameter space besides the global minimum of MSE at the default model parameter.
LHPP is associated with favorable survival across diverse cancers with tumor suppressor properties in stomach adenocarcinoma: a pan-cancer analysis
Authors: Li Wenhan , Wang Danfang , Li Jianhui 
Published: July 2022

Abstract
Background
The down-regulation of LHPP has been reported as a tumor suppressor gene in several malignancies. This study aims to explore the potential roles of LHPP across 33 types of tumors.
Methods
The TCGA database was mainly used to investigate the correlation of LHPP expression pattern with clinical prognosis and other function related gene sets. Additionally, immunohistochemistry and western blot assay were used to detect the expression level of LHPP in tissue samples for stomach adenocarcinoma (STAD) patients. We next established STAD cell lines with exogenous silencing/ overexpression of LHPP. qRT-PCR, western blot assay, CCK-8 assay and colony formation assays were performed for cell function identifications.
Results
LHPP was widely down-regulated in tumor tissues compared with normal tissues in pan-cancer across 13 cancer types. The expression level of LHPP was associated with favorable survival across diverse cancers. Moreover, we observed that LHPP expression was significantly correlated with immune infiltration, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repair genes (MMRs), and DNA methyltransferases in many tumors. Validation in clinical tissue samples confirmed LHPP was down-regulated in STAD. Functional experiments demonstrated that LHPP suppressed cell proliferation of STAD via down-regulating p‑PI3K/p‑AKT expression levels.
Conclusion
Our first pan-cancer study provided insight into the role of LHPP in tumorigenesis. Besides, LHPP may acts as a tumor suppressor gene in STAD.
Treatment of ruptured rectal artery aneurysm in a patient with neurofibromatosis
Authors: Nemoto Hidehiko , Mori Kensaku , Takei Yohei , Kikuchi Shunsuke , Hoshiai Sodai , Yamamoto Yoshiyuki , Nakajima Takahito 
Published: July 2022

Abstract
Background: Superior rectal artery (SRA) aneurysms are rare. Although melena is the most common symptom, it has not been observed in cases of aneurysms located in the SRA trunk. Here, we report a case of a ruptured SRA trunk aneurysm successfully treated with coil embolization. Including our case, three of the four reported cases of SRA trunk aneurysms were related to neurofibromatosis type 1 (NF1). Case presentation: A 52-year-old woman with NF1 was referred to our hospital for the investigation of an abdominal mass with back pain. She had previously undergone a blood transfusion at another hospital for anemia without melena. Computed tomography angiography revealed a ruptured SRA trunk aneurysm measuring 3 cm in diameter and surrounded by a retroperitoneal hematoma. The aneurysm was isolated by embolizing the SRA trunk distally and proximally. Distal embolization was performed retrogradely from the internal iliac artery (IIA) via the middle rectal artery (MRA)-SRA anastomosis because the antegrade approach from the inferior mesenteric artery (IMA) failed. To our knowledge, this is the first case of successful coil embolization of an IMA branch through the IIA.Conclusion: SRA trunk aneurysms are rare; however, they are frequently associated with NF1. Antegrade distal embolization beyond the aneurysm is sometimes difficult to achieve. In such cases, a retrograde approach via MRA-SRA anastomosis can be the choice for isolating SRA trunk aneurysms.
Anchoring carboxylated pineapple peel cellulose and EDTA onto magnetic (3-aminopropyl)triethoxysilane to effective diminish copper(II) and zinc(II) from polluted water
Authors: Sayahi Hanan , Asadabadi Simin 
Published: July 2022

Abstract
In recent decades, remediation of polluted water has shifted towards the enhancement of environmentally friendly adsorbents with robust stability. In this regard, a biosorbent was designed and synthesized by anchoring carboxylated pineapple peel cellulose and ethylenediaminetetraacetic acid onto amino-silane modified Fe3O4 nanoparticles. A vast number of carboxyl and amino active groups in the structure of biosorbent led to the efficient adsorption of Cu2+ and Zn2+ from polluted water. The biosorbent was characterized by FE-SEM, TEM, EDX, XRD, FT-IR, BET, VSM and TGA techniques. The influence of pH, temperature and biosorbent dosage on the cation removal was investigated by Central Composite Design. The maximum uptake% was 93.18 and 96.30% for Cu2+ and Zn2+, respectively. Coordination and electrostatic attraction were proposed as the main reasons for the removal of heavy metal cations. Astonishingly, the adsorption of Zn2+ was ultrafast and completed within 40 s and it took 420 s for removal of Cu2+. External surface biosorption, the intra-particle diffusion and the diffusion through the smaller pores and slits, in sequence, were the dominant steps in the adsorption of each cation. Modified pseudo-n-order and modified Langmuir-Freundlich were the best models to describe kinetics and equilibrium results. The maximum adsorption capacity of biosorbent for Zn2+ was higher than one for Cu2+ (153.9 versus 149.4 mg·g-1). Besides, the regenerating of biosorbent was successfully accomplished for five cycles using a solution of EDTA in the presence of ultrasonic waves.
Microstream end-tidal carbon dioxide and capillary carbon dioxide in mechanically ventilated extreme preterm neonates: a methods comparison study
Authors: Boyapally Vamshi Krishna , Sundaram Venkataseshan , Kumar Praveen , Kumar Jogender 
Published: July 2022

Abstract
Purpose: End-tidal carbon dioxide (ETCO2) is not routinely used in mechanically ventilated extreme preterm neonates due to the low tidal volume generated. Microstream devices potentially alleviate this problem as they need small sampl volumes. We compared micro stream ETCO2 and arterialized capillary PCO2 (PacCO2) in mechanically ventilated, extreme premature neonates for their correlation and agreement. Methods: ETCO2 and PacCO2 were time matched. Repeated measures correlation coefficient was calculated, and Bland-Altman agreement plot was created. A linear mixed-effects model was developed.Results: In the 49 pairs of ETCO2 and PacCO2, a moderately positive repeated measures correlation coefficient was observed [0.63 (0.4-0.81)]. A mean average monotonic bias of 22.6 mmHg was observed between PacCO2 and ETCO2. After controlling for mean airway pressure and respiratory rate, ETCO2 significantly predicted PacCO2 (b = 0.94 (0.39-1.5)).Conclusions: Micro stream ETCO2 and PacCO2 showed a moderately positive correlation but a high non-linear bias in mechanically ventilated, extreme preterm neonates in the first week of life. More research in larger and clinically diverse samples is required before routine clinical use
Pretreatment with abscisic acid accompanied by sucrose improves callus survival after cryopreservation of hazel (Corylus avellana L.) by desiccation
Authors: nanas shiva mojarrad , Mokhtassi-Bidgoli Ali 
Published: July 2022

Abstract
A simple cryopreservation method (desiccation) is examined on Corylus avellana L. callus in current study. The effect of abscisic acid (ABA) concentration, preculture length on Murashige and Skoog (MS) medium containing ABA + 10% sucrose, and storage length in liquid nitrogen (LN) on callus survival rate were investigated. After cryo-storage, the calli were heated at 40°C followed by cold tap water for one minute, then transferred to the recovery medium. Calli survival was assessed 6–8 weeks after exiting from LN. There was a significant difference between ABA concentrations and different culture periods on medium containing mentioned hormone in survival rate but no significant difference was observed between the storage periods in LN. In the present study, the highest survival rate (45/79%) was obtained in both the treatment of 20 days preculture on medium containing 2 mg l− 1 ABA following 120 minutes desiccation-one day storage in LN and 150 minutes desiccation-one month storage in LN.