bluuebunny/crossref_metadata_2025_split huggingface dataset:
PCOS: a Chronic Disease That Fails to Produce Adequately Specialized Pro-resolving Lipid Mediators (SPMs).
Authors: Regidor Pedro-Antonio , Rosa Xavier de la , Mueller Anna , Sailer Manuela , Santos Fernando Gonzalez , Banzo Rafael Gracia , Rizo Jose Miguel 
Published: January 2022

Abstract
Introduction: Polycystic Ovary Syndrome (PCOS) is an endocrinologic disorder that affects 5-15 % of women of their reproductive age and is a frequent cause of infertility. Major symptoms include hyperandrogenism, ovulatory dysfunction, and often obesity and/or insulin resistance. PCOS also represents a state of chronic low-grade inflammation that is closely interlinked with the metabolic features. "Classical" pro-inflammatory lipid mediators like prostaglandins (PG), leukotrienes (LT), or thromboxanes (TX) are derived from arachidonic acid (AA) and are crucial for the initial response. Resolution processes are driven by four families of so-called specialized pro-resolving mediators (SPMs): resolvins, maresins, lipoxins, and protectins. The study aimed to establish lipid mediator profiles of PCOS patients compared to healthy women to identify differences in their resolutive and pro-inflammatory lipid parameters. Material and Methods: Fifteen female patients (18-45 years) were diagnosed with PCOS according to Rotterdam criteria, and five healthy women, as comparator group, were recruited for the study. The main outcome measures were: Pro-inflammatory lipid mediators (PG, LT, TX) and their precursor AA; SPMs (Resolvins, Maresins, Protectins, Lipoxins), their precursors EPA, DHA, DPA, and their active biosynthesis pathway intermediates (18-HEPE, 17-HDHA, 14-HDHA).Results: The level of pro-inflammatory parameters in serum was significantly higher in PCOS-affected women. The ratio [(sum of pro-inflammatory molecules) / (sum of SPMs plus hydroxylated intermediates)] reflecting the inflammatory state was significantly lower in the group of healthy women.Conclusion: There is a strong pro-inflammatory state in PCOS patients. Further research will clarify whether supplementation with SPMs or their precursors may improve this state.
A Highly Fluorescent Pyrene-based Sensor for Selective Detection of Fe3+ Ion in Aqueous Medium: DFT and Molecular Docking Studies
Authors: Rasin Puthiyavalappil , Mathew Merlin Mary , Manakkadan Vipin , Palakkeezhillam Vishnunarayanan Namboothiri Vadakkedathu , Anandaram Sreekanth 
Published: January 2022

Abstract
In this work, we introduce a highly selective and sensitive fluorescent sensor based on pyrene derivative for Fe(III) ion sensing in DMSO/water media. 2-(pyrene-2-yl)-1-(pyrene-2-ylmethyl)-1H-benzo[d]imidazole (PEBD) receptor was synthesized via simple condensation reaction and confirmed by spectroscopic techniques. The receptor exhibits fluorescence quenching in the presence of Fe(III) ions at 440 nm. ESI-MS and Job’s method were used to confirm the 1:1 molar binding ratio of the receptor PEBD to Fe(III) ions. Using the Benesi-Hildebrand equation the binding constant value was determined as 8.485×103 M-1. Furthermore, the limit of detection (LOD, 3σ/K) value was found to be 1.81µM in DMSO/water (95/5, v/v) media. According to the Environmental Protection Agency (EPA) of the United States, it is lower than the acceptable value of Fe3+ in drinking water (0.3 mg/L). The presence of 14 other metal ions such Co2+, Cr3+, Cu2+, Fe2+, Hg2+, Pb2+, K+, Ni2+, Mg2+, Cd2+, Ca2+, Mn2+, Al3+, and Zn2+ did not interfere with the detection of Fe(III) ions. Computational studies of the receptor PEBD were carried out with density functional theory (DFT) using B3LYP/ 6-311G (d, p), LANL2DZ level of theory. Finally, molecular docking studies have been performed to investigate the Cytochrome P450 1A1(CYP1A1) protein inhibitory action of the receptor PEBD.
Preoperative S-1 Therapy for Squamous Cell Carcinoma of The Head and Neck During the Waiting Period Before Surgery
Authors: Matsuki Takashi , Fushimi Chihiro , Miyamoto Shunsuke , Takahashi Hideaki , Masubuchi Tatsuo , Tada Yuichiro , Miura Kouki , Kamata Shin-etsu , Kano Koichi , Tsutsumi Shohei , Momiyama Kaho , Yamashita Taku 
Published: January 2022

Abstract
Background: In patients with squamous cell carcinoma of head and neck (SCCHN), delayed surgery can result in poorer postoperative function and prognosis due to the growth of the tumor and the extended surgery. Further, delay may even make the tumor unresectable. To prevent tumor growth during the waiting period before surgery, S-1 has been administrated preoperatively at several facilities in Japan. To date, however, the safety and efficacy of preoperative S-1 remains unclear.Methods: We conducted a retrospective cohort study of 118 patients with SCCHN treated with S-1 before radical surgery at 2 institutions in Japan. We evaluated the safety of S-1 therapy, which was evaluated by the incidence of grade 3 or greater adverse events (AEs). The rate of achievement of the non-growth of tumors was also calculated.Results: Regarding safety, 125 AEs of all grades were recorded in 71 patients (60%). Of these, grade 3 AEs were detected in 3 patients (3%), and no grade 4 or 5 AEs occurred. The non-growth rate of primary lesions and lymph node metastases was 89% and 85%, respectively. Conclusion: Our data showed that preoperative S-1 therapy might be useful with acceptable toxicity on an outpatient basis in patients with SCCHN.
Early Markers of Cardiovascular Disease Associated With Clinical Data and Autosomal Ancestry in Patients With Type 1 Diabetes: A Cross-sectional Study in an Admixed Brazilian Population.
Authors: Reis Roberta Maria Duailibe Ferreira Reis , Silva Dayse Aparecida , Tavares Maria da Glória , Nascimento Gilvan Cortês , Damianse Sabrina da Silva Pereira , Rocha Viviane Chaves de Carvalho , Almeida Ana Gregória , Lago Débora Cristina Ferreira , Rodrigues Vandilson , Magalhães Marcelo , Sobral Carla Souza , Parente Conceição , França Joana , Ribeiro Jacqueline , Ferraz Paulo Cézar Dias , Azulay Carlos , Gomes Marília Brito , Faria Manuel dos Santos , Azulay Rossana Santiago de Sousa 
Published: January 2022

Abstract
BackgroundPatients with type 1 diabetes (T1D) have a higher risk of developing cardiovascular disease (CVD), which is a major cause of death in this population. The objective of this study was to investigate early markers of CVD associated with clinical data and autosomal ancestry in T1D patients from an admixed Brazilian population. MethodsA cross-sectional study was conducted with 99 T1D patients. The early markers of CVD included the ankle-brachial index (ABI), coronary artery calcium score (CACS), and carotid Doppler sonography. Demographic, clinical, and serum data were collected. A panel of autosomal informational insertion/deletion ancestry markers was used to estimate the individual proportions of European, African, and Amerindian ancestry.ResultsThe study sample had a mean age of 27.6 years and 14.4 years of duration of T1D. The prevalence of alterations in early CVD markers was: ABI (< 0,9) 19.6%, CACS (> 0 +) 4.1%, and carotid Doppler 5.0%. There was significant agreement between CACS and carotid Doppler, and these were correlated with traditional risk factors for CVD. There was a predominance of European ancestry (47.3%), followed by African (28%) and Ameridian (24.7%). There were no association between early CVD markers and autosomal ancestry proportions.ConclusionThe ABI was useful in the early identification of CVD in asymptomatic young patients with T1D and with a short duration of disease, and showed agreement with the carotid Doppler. Although CACS and carotid Doppler are non-invasive tests, carotid Doppler is more cost-effective, and both have limitations in screening for CVD in young patients with a short duration of T1D. We did not find a statistically significant relationship between autosomal ancestry proportions and early CVD markers in an admixed Brazilian population.
Identification of Molecular Biomarkers Associated With Stroke Progression Using WGCNA and SVM-RFE
Authors: Liu Si-tong , Zhang You , Wu Xin-gui , Lu Chang-xing , Hu Qi-Ping 
Published: January 2022

Abstract
Background: Stroke is the second most common cause of death worldwide and the leading cause of long-term severe disability with neurological impairment worsening within hours after stroke onset and being especially involved with motor function. So far, there are no established and reliable biomarkers to prognose stroke. Early detection of biomarkers that can prognose stroke is of great importance for clinical intervention and prevention of clinical deterioration of stroke.Methods: TGSE119121 dataset was retrieved from the Gene Expression Integrated Database (Gene Expression Omnibus, GEO) and weighted gene co-expression network analysis (WGCNA) was conducted to identify the key modules that could regulate disease progression. Moreover, functional enrichment analysis was conducted to study the biological functions of the key module genes. The GSE16561 dataset was further analyzed by the Support Vector Machines coupled with Recursive Feature Elimination (SVM-RFE )algorithm to identify the top genes regulating disease progression. The hub genes revealed by WGCNA were associated with disease progression using the receiver operating characteristic curve (ROC) analysis. Subsequently, functional enrichment of the hub genes was performed by deploying gene set variation analysis (GSVA). The changes at gene level were transformed into the changes at pathway level to identify the biological function of each sample. Finally, the expression level of the hub gene in the rat infarction model of MCAO was measured using RT-qPCR for validation. Results: WGCNA analysis revealed four hub genes: DEGS1, HSDL2, ST8SIA4 and STK3. The result of GSVA showed that the hub genes were involved in stroke progression by regulating the p53 signal pathway, the PI3K signal pathway, and the inflammatory response pathway. The results of RT-qPCR indicated that the expression of the four HUB genes was increased significantly in the rat model of MCAO.Conclusion: Several genes, such as DEGS, HSDL2, ST8SIA4 and STK3, were identified and associated with the progression of the disease. Moreover, it was hypothesized that these genes may be involved in the progression stroke by regulating the P53 signal, the PI3K signal, and the inflammatory response pathway, respectively. These genes have potential prognostic value and may serve as biomarkers for predicting stroke progression. The early identification of the patients at risk of progression is essential to prevent clinical deterioration and provide a reference for future research.
Immediate Postoperative Single-Dose Intravenous Ferric Carboxymaltose After Autologous Free-Flap Breast Reconstruction: A Prospective Cohort Study With Propensity Score Matching
Authors: Park Joseph Kyu-hyung , Lee Seungjun , Heo Chan Yeong , Jeong Jae Hoon , Myung Yujin 
Published: January 2022

Abstract
Intravenous ferric carboxymaltose (IV-FCM) can effectively correct perioperative anemia in patients undergoing major surgeries. However, its efficacy and side effects in patients undergoing free flap-based breast reconstruction are yet to be investigated. Starting from year 2020, patients with breast cancer undergoing abdominal free flap-based breast reconstruction were injected 500 mg of IV-FCM immediately post-operation. Propensity-matched 82 IV-FCM injected (study group) and 164 historical control group patients were analyzed for transfusion rates, changes in hematological parameters, and flap or donor-site related complications. The major and minor complication rates related to the operation site were similar between the two groups. There was no significant difference in the transfusion rate between the two groups (p = 0.71). However, the total amount of transfusion required was significantly higher in the historical control group (p = 0.02) than in the study group. Additionally, the historical control group showed a significantly higher drop in red blood cell count, hemoglobin, and hematocrit levels from postoperative days 1 to 2 and 2 to 3 compared to the study group. Immediate postoperative use of IV-FCM in free flap-based breast reconstruction was well tolerated by patients, reduced overall transfusion volume, and promoted faster postoperative recovery of hematological parameters.
Response Surface Grey Relational Analysis On The Manufacturing of High Grade Biomedical Ti-13Zr-13Nb
Authors: Abifarin Johnson Kehinde , Fidelis Fredah Batale , Abdulrahim Moshood Yemi , Oyedeji Elijah Oyewusi , Nkwuo Tochukwu , Prakash Chander 
Published: January 2022

Abstract
Optimization of the manufacturing conditions with more than one performance characteristics have been a thing of concern, especially for Response Surface Method (RSM) optimization. Hence, this study addressed this challenge by reanalyzing a data presented in a previous study using grey relational analysis (GRA) and regression analysis. Central Composite Design (CCD) of RSM with high and low values of manufacturing conditions; voltage (50, 70) V, current (8, 16) A, pulse ON time (6, 10) μs, and pulse OFF time (7, 11) μs. The manufacturing conditions for optimal biomedical Ti-13Zr-13Nb alloy were obtained to be 50V voltage, 8A current, 6 μs pulse ON time, and 11 μs pulse OFF time. It was also revealed that the mathematical model was very efficient because the modeled GRG was in consonant with the experimental one. In addition, it was also established that current was the most significant manufacturing condition with a contribution of 47.27%. Voltage, factors interactions and residual error were insignificant on the GRG value of the titanium alloy. In conclusion, it can be deduced that the a small value of voltage within the considered settings could be used to manufacture better grade Ti-13Zr-13Nb alloy and also the small value of residual error showed the high manufacturability of the material.
Spatial-dependent Quantum Dot-photon Entanglement via Tunneling Effect
Authors: joughi Yaser Delir ghaleh , Sahrai Mostafa 
Published: January 2022

Abstract
Utilizing the vortex beams, we investigate the entanglement between the triple-quantum dot molecule and its spontaneous emission field. We present the spatially dependent quantum dot-photon entanglement created by Laguerre-Gaussian (LG) fields. The degree of position-dependent entanglement (DEM) is controlled by the angular momentum of the LG light and the quantum tunneling effect created by the gate voltage. Various spatial-dependent entanglement distribution is reached just by the magnitude and the sign of the orbital angular momentum (OAM) of the optical vortex beam.
Unmet Health Need Among Roma: Visual Acuity and the Use of Vision Correcting Aids in the Hungarian Roma Population
Authors: Losonczy Gergely , Piko Peter , Klevering B. Jeroen , Kosa Zsigmond , Sandor Janos , Adany Roza 
Published: January 2022

Abstract
The Roma population is the largest transnational ethnic minority group in Europe often facing socioeconomic inequalities and various health problems. In the present study we investigated the visual acuity and its affecting factors along with spectacle use of the Roma in comparison with the general population in Hungary.A cross-sectional survey was carried out including 832 participants aged 20 to 64 years. We recorded the visual acuity, anthropometric, demographic, socioeconomic and health-related data of each individual. Although the average uncorrected visual acuity was somewhat higher, the use of a visual aid was significantly less frequent in the Roma population, especially in the group with a visual acuity below 0.5 in both eyes (14.3% vs 77.1%, p<0.001). Age, abdominal obesity and disturbances of the carbohydrate metabolism had a negative impact on visual acuity in both populations, however, the latter was a much stronger risk factor in the Roma population (OR: 5.789, 95% CI: 2.239-14.964, p<0.001) than in the general population (OR: 2.075, 95% CI: 1.097-3.926, p=0.025). Our results show serious unmet health needs within the Roma population, which calls for public health programs to improve poor primary care indicators on regular eye examination, and a much more rigorous diabetes control.
Oxaliplatin-Induced Peripheral Neuropathy Can Be Minimized By Pressurized Regional Intravascular Delivery In An Orthotopic Murine Pancreatic Cancer Model.
Authors: Narayanan Jayanth Surya Narayanan Shankara , Frizzi Katie , Erdem Suna , Ray Partha , Jaroch David , Cox Bryan , Katz Steven , Vicente Diego , White Rebekah R. 
Published: January 2022

Abstract
Purpose: There is a great need to reduce the toxicity of chemotherapy used in the management of pancreatic ductal adenocarcinoma (PDAC). Here we explore if regional pressurized delivery of oxaliplatin can minimize peripheral neuropathy in mice.Methods: We used an orthotopic PDAC mouse model and delivered a single dose of oxaliplatin through the portal vein using a pressure-enabled system (pancreatic retrograde venous infusion, PRVI). We analyzed the effects of PRVI on tumor burden and peripheral neuropathy using histopathological and functional assays.Results: Tumor weights in mice treated with 2 mg/Kg oxaliplatin using PRVI were significantly lower than in mice treated with the same dose systemically. This resulted in reduced peripheral neuropathy signatures in PRVI mice compared to the 20 mg/Kg systemic dose required to achieve similar tumor control.Conclusion: Regional delivery of highly cytotoxic agents using PRVI can reduce the therapeutic dose of these drugs, thereby lowering toxic side effects.
Bacteriological Identification among Patients Attending at Jimma University Veterinary Clinic
Authors: Ayana Gelana Urgesa , Tesfaye Boka 
Published: January 2022

Abstract
Findings of conducted study showed that the predominant bacteria in Jimma University Veterinary Clinic. Among predominant bacteria identified includes; Escherichia coli 46 (34.3%), Klebsiella 23(17.2%), followed by Staphylococcus aureus 40(29.9%) and Streptococcus spp 25(18.7%). Among 4 species of bacteria isolated, 2 were gram negative and 2 of them was gram positive bacteria. Among the antibiotic susceptibility test, Staphylococcus aureus showed the total resistant on penicillin while tetracycline showed to have the highest sensitivity. Escherichia coli showed the total resistant on gentamicin (100%). Veterinary Clinic is at risk of acquiring bacterial infections due to the presence of mentioned bacteria during this study. The big issue is that these infections resist to antibiotics used in treatment according to results of the study. Health care workers in Veterinary Clinic should improve hygiene to control clinic acquired infections and much attention should be done during diagnosis to improve treatment of patients.
Giant bladder stone resulting in acute kidney injury; a case report and review of literature
Authors: Gebrehiwot Fitsum Gebreegziabher , Adem Mubarek Bargicho , Issack Feysel Hassen , Gebresellasie Kaleab Habtemichael , Mummed Ferid Ousman , Hassen Ibsa Kedir 
Published: January 2022

Abstract
A giant bladder stone is very rare in adults. We report a case of giant bladder stone causing acute kidney injury in a 23-year-old male, who presented with lower urinary tract symptoms (LUTS) characterized by both irritative and obstructive LUTS. In addition, he also had episodes of reddish urine for the past decade. A non-contrast-enhanced CT scan was used for the diagnosis. Open cystolithotomy was performed and a 500g weighing stone was removed. He developed a superficial surgical site infection which was treated with wound care. He was discharged improved. Improvement in symptoms and serum creatinine was noted on follow-up.
Evaluation of Preventive Measures and Forecasting of COVID-19 Infection: Case Study Egypt.
Authors: Khaled Safaa A A , Hafez Ahmed A A 
Published: January 2022

Abstract
Background

COVID-19 is a highly infectious disease caused by SARS-CoV-2. This article assessed the effectiveness of preventive measures of COVID-19 infection, including social distancing (SD) and quarantine (Q) of patients and contacts in Egypt.
Methods

A simple model was developed to predict the infection rate without preventive measures. The article utilizes fertile meta- heuristic technique and particle swarm optimization (PSO), to predict the growth of the disease.
Results

A correlation between the predicted and actual infected cases, validated the proposed forecasting algorithm. Preventive measures together with the Egyptian Government stay home order reduced 98% of expected infections. PSO analyses showed that infection and death rates will continue to increase particularly with lifting these restrictive preventive measures.
Conclusions

The advised PSO model could predict COVID-19 infection and death rates with high degree of accuracy. This prediction model could help health authorities in decision making.
Using Ultrasound Guided Needle Biopsy in Combination With GeneXpert MTB/RIF to Diagnose Epididymal Tuberculosis: a Study of 12 Cases
Authors: Qinghu Yan , Jia Cui , Bo Liang , Binbin Feng , Wuzhang Wang , Dexin Yu , Yan'an Zhang 
Published: January 2022

Abstract
Objective: This study aims to investigate the diagnostic utility of percutaneous ultrasound-guided needle biopsy in combination with GeneXpert MTB/RIF for epididymal tuberculosis.Methods: Between March 2019 to March 2021, specimens obtained from 12 patients with confirmed epididymal tuberculosis by ultrasound guided needle biopsy were sent for pathology and laboratory examination at the Shandong Public Health Clinical Center. The laboratory examination included acid-fast staining, Mycobacterium tuberculosis culture by BACTEC MGIT 960, and the GeneXpert MTB/RIF test. The diagnosis and complications were comprehensively analyzed.Results: Among the 12 cases, seven cases had granulomatous inflammation and necrotic tissue, four cases had chronic inflammatory cells and necrotic tissue, and one case had chronic inflammatory cell infiltration. Furthermore, among the 12 patients, five patients were positive for Mycobacterium tuberculosis culture, two patients were positive for the acid-fast staining, and twelve patients were positive for the GeneXpert MTB/RIF assay. The sensitivity and specificity of the acid-fast staining, Mycobacterium tuberculosis culture and GeneXpert MTB/RIF in the diagnosis of epididymis tuberculosis were 16.67% and 100.00%, 41.67% and 100.00%, and 100.00% and 50.00%, respectively. The diagnostic value analysis of the three detection techniques indicated that the GeneXpert MTB/RIF technique (Kappa=0.63, AUC=0.75) is superior to the Mycobacterium tuberculosis culture (Kappa=0.17, AUC =0.71) and acid-fast staining (Kappa=0.05, AUC=0.58). Conclusion: Ultrasound guided percutaneous biopsy combined with GeneXpert MTB/RIF assay is extremely useful for diagnosing epididymitis tuberculosis and determining rifampin resistance, with superior sensitivity, specificity and AUC value.