bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health effects associated with vegetable consumption: a Burden of Proof study
Authors: Stanaway Jeffrey D. , Afshin Ashkan , Ashbaugh Charlie , Bisignano Catherine , Brauer Michael , Ferrara Giannina , Garcia Vanessa , Haile Demewoz , Hay Simon I. , He Jiawei , Iannucci Vincent , Lescinsky Haley , Mullany Erin C. , Parent Marie C. , Serfes Audrey L. , Sorensen Reed J. D. , Aravkin Aleksandr Y. , Zheng Peng , Murray Christopher J. L. 
Published: October 2022

AbstractPrevious research suggests a protective effect of vegetable consumption against chronic disease, but the quality of evidence underlying those findings remains uncertain. We applied a Bayesian meta-regression tool to estimate the mean risk function and quantify the quality of evidence for associations between vegetable consumption and ischemic heart disease (IHD), ischemic stroke, hemorrhagic stroke, type 2 diabetes and esophageal cancer. Increasing from no vegetable consumption to the theoretical minimum risk exposure level (306–372 g daily) was associated with a 23.2% decline (95% uncertainty interval, including between-study heterogeneity: 16.4–29.4) in ischemic stroke risk; a 22.9% (13.6–31.3) decline in IHD risk; a 15.9% (1.7–28.1) decline in hemorrhagic stroke risk; a 28.5% (−0.02–51.4) decline in esophageal cancer risk; and a 26.1% (−3.6–48.3) decline in type 2 diabetes risk. We found statistically significant protective effects of vegetable consumption for ischemic stroke (three stars), IHD (two stars), hemorrhagic stroke (two stars) and esophageal cancer (two stars). Including between-study heterogeneity, we did not detect a significant association with type 2 diabetes, corresponding to a one-star rating. Although current evidence supports increased efforts and policies to promote vegetable consumption, remaining uncertainties suggest the need for continued research.
Adult-born dentate granule cells promote hippocampal population sparsity
Authors: McHugh Stephen B. , Lopes-dos-Santos Vítor , Gava Giuseppe P. , Hartwich Katja , Tam Shu K. E. , Bannerman David M. , Dupret David 
Published: October 2022

AbstractThe dentate gyrus (DG) gates neocortical information flow to the hippocampus. Intriguingly, the DG also produces adult-born dentate granule cells (abDGCs) throughout the lifespan, but their contribution to downstream firing dynamics remains unclear. Here, we show that abDGCs promote sparser hippocampal population spiking during mnemonic processing of novel stimuli. By combining triple-(DG-CA3-CA1) ensemble recordings and optogenetic interventions in behaving mice, we show that abDGCs constitute a subset of high-firing-rate neurons with enhanced activity responses to novelty and strong modulation by theta oscillations. Selectively activating abDGCs in their 4–7-week post-birth period increases sparsity of hippocampal population patterns, whereas suppressing abDGCs reduces this sparsity, increases principal cell firing rates and impairs novel object recognition with reduced dimensionality of the network firing structure, without affecting single-neuron spatial representations. We propose that adult-born granule cells transiently support sparser hippocampal population activity structure for higher-dimensional responses relevant to effective mnemonic information processing.
U(VI) removal from diluted aqueous systems by sorption–flotation
Authors: Constantin Carolina , Popescu Ioana-Carmen , Oprea Ovidiu , Stoica Ligia 
Published: October 2022

AbstractThe legacies of past uranium mining and milling activities for nuclear fuel fabrication continue to be a cause of concern and require assessment and remedial action for researchers worldwide. The discharge of uranium contaminated water into the environment is a matter of regulation (World Health Organization, WHO—15 μg/L, Romanian Legislation, RO—21 μg/L), environment and health. Therefore, various removal technologies of U(VI) from diluted aqueous solutions include chemical precipitation, ion exchange, adsorption, immobilization on zero-valent iron nanoparticles, etc. have been extensively applied. Our previous research has studied the removal of U(VI) from diluted aqueous systems such as mine waters using Fe0-based nanomaterials synthesized in the laboratory (NMS) (Crane et al. in Water Res 45:2391–2942, 2011). The carbonate rich aqueous system was treated with NMS to remove U(VI). It was observed that after half an hour of reacting time only about 50% was removed due to its high tendency to form stable soluble carbonated complexes. Considering that, the present article aims to investigate the Sorption/Flotation technique, by using a sorbent generated in situ Fe2O3· nH2O and sodium oleate surfactant to remove U(VI) from diluted aqueous systems and to update the knowledge on the mechanism of process. In order to determine the removal efficiency of U(VI), the influencing factors were studied: pH, sorbent dose, surfactant concentration, contact time, stirring rate, the U(VI) concentration, air pressure in pressurized water recipient, and the effect of some accompanying heavy metals ions (Cu(II), Cr(VI), and Mo(VI)). The removal efficiency (%R) was monitored and its maximum values allowed to establish the optimal separation parameters (the established process parameters), which were validated on real mine water samples (MW). High U (VI) removal efficiencies %R > 98% were obtained. The Sorption/ Flotation technique was applied to remove U(VI) from two types of real mine water samples, namely ”simple” and ”pre-treated with NMS”, respectively. For the mine water samples pre-treated with NMS, it worked in two variants: with and without pH correction. For pH range = 7.5–9.5, molar ratios [U(VI)] : [Fe(III)] = 1 : 75, [U(VI)] : [NaOL] = 1 : 1 × 10–2, contact time 30 min., stirring speed 250 RPM, initial concentration of U(VI) 10 mg·L−1, air pressure in pressurized water recipient p = 4 × 105 N·m−2 is obtained %R > 98%. It has been found that Sorption / Flotation can function with good %R values as a stand—alone operation or in tandem with NMS pre-treatment of mine water and pH adjustment proved to be highly efficiency (CU(VI) < 1·10–3 mg·L−1).
Gut microbiome insights from 16S rRNA analysis of 17-year periodical cicadas (Hemiptera: Magicicada spp.) Broods II, VI, and X
Authors: Brumfield Kyle D. , Raupp Michael J. , Haji Diler , Simon Chris , Graf Joerg , Cooley John R. , Janton Susan T. , Meister Russell C. , Huq Anwar , Colwell Rita R. , Hasan Nur A. 
Published: October 2022

AbstractPeriodical cicadas (Hemiptera: Magicicada) have coevolved with obligate bacteriome-inhabiting microbial symbionts, yet little is known about gut microbial symbiont composition or differences in composition among allochronic Magicicada broods (year classes) which emerge parapatrically or allopatrically in the eastern United States. Here, 16S rRNA amplicon sequencing was performed to determine gut bacterial community profiles of three periodical broods, including II (Connecticut and Virginia, 2013), VI (North Carolina, 2017), and X (Maryland, 2021, and an early emerging nymph collected in Ohio, 2017). Results showed similarities among all nymphal gut microbiomes and between morphologically distinct 17-year Magicicada, namely Magicicada septendecim (Broods II and VI) and 17-year Magicicada cassini (Brood X) providing evidence of a core microbiome, distinct from the microbiome of burrow soil inhabited by the nymphs. Generally, phyla Bacteroidetes [Bacteroidota] (> 50% relative abundance), Actinobacteria [Actinomycetota], or Proteobacteria [Pseudomonadota] represented the core. Acidobacteria and genera Cupriavidus, Mesorhizobium, and Delftia were prevalent in nymphs but less frequent in adults. The primary obligate endosymbiont, Sulcia (Bacteroidetes), was dominant amongst core genera detected. Chryseobacterium were common in Broods VI and X. Chitinophaga, Arthrobacter, and Renibacterium were common in Brood X, and Pedobacter were common to nymphs of Broods II and VI. Further taxonomic assignment of unclassified Alphaproteobacteria sequencing reads allowed for detection of multiple copies of the Hodgkinia 16S rRNA gene, distinguishable as separate operational taxonomic units present simultaneously. As major emergences of the broods examined here occur at 17-year intervals, this study will provide a valuable comparative baseline in this era of a changing climate.
Enterotypes in asthenospermia patients with obesity
Authors: Jiao Jiao , Xu Peng , Wang Xiaobin , Xing Ze , Dong Sitong , Li Gaoyu , Yao Xinrui , Guo Renhao , Feng Tao , Yao Weifan , Pan Bochen , Zhu Xuan , Wang Xiuxia 
Published: October 2022

AbstractThe essence of enterotypes is stratifying the entire human gut microbiome, which modulates the association between diet and disease risk. A study was designed at the Center of Reproductive Medicine, Shengjing Hospital of China Medical University and Jinghua Hospital of Shenyang. Prevotella and Bacteroides were analyzed in 407 samples of stool, including 178 men with enterotype B (61 normal, 117 overweight/obese) and 229 men with enterotype P (74 normal, 155 overweight/obese). The ratio between Prevotella and Bacteroides abundance, P/B, was used as a simplified way to distinguish the predominant enterotype. In enterotype P group (P/B ≥ 0.01), obesity was a risk factor for a reduced rate of forward progressive sperm motility (odds ratio [OR] 3.350; 95% confidence interval [CI] 1.881–5.966; P < 0.001), and a reduced rate of total sperm motility (OR 4.298; 95% CI 2.365–7.809; P < 0.001). Obesity was also an independent risk factor (OR 3.131; 95% CI 1.749–5.607; P < 0.001) after adjusting follicle-stimulating hormone. In enterotype P, body mass index, as a diagnostic indicator of a reduced rate of forward progressive sperm motility and a decreased rate of decreased total sperm motility, had AUC values of 0.627 (P = 0.001) and 0.675 (P < 0.0001), respectively, which were significantly higher than the predicted values in all patients. However, in enterotype B group (P < 0.01), obesity was not a risk factor for asthenospermia, where no significant difference between obesity and sperm quality parameters was observed. This study is tried to introduce enterotypes as a population-based individualized classification index to investigate the correlation between BMI and asthenospermia. In our study, overweight/obese men with enterotype P were found to have poorer sperm quality. however, sperm quality was not associated with overweight/obese in men with enterotype B. Thereof, BMI is a risk factor for asthenospermia only in men with enterotype P, but not in men with enterotype B.
Epidermal growth factor receptor cascade prioritizes the maximization of signal transduction
Authors: Kiso-Farnè Kaori , Tsuruyama Tatsuaki 
Published: October 2022

AbstractMany studies have been performed to quantify cell signaling. Cell signaling molecules are phosphorylated in response to extracellular stimuli, with the phosphorylation sequence forming a signal cascade. The information gain during a signal event is given by the logarithm of the phosphorylation molecule ratio. The average information gain can be regarded as the signal transduction quantity (ST), which is identical to the Kullback–Leibler divergence (KLD), a relative entropy. We previously reported that if the total ST value in a given signal cascade is maximized, the ST rate (STR) of each signaling molecule per signal duration (min) approaches a constant value. To experimentally verify this theoretical conclusion, we measured the STR of the epidermal growth factor (EGF)-related cascade in A431 skin cancer cells following stimulation with EGF using antibody microarrays against phosphorylated signal molecules. The results were consistent with those from the theoretical analysis. Thus, signaling transduction systems may adopt a strategy that prioritizes the maximization of ST. Furthermore, signal molecules with similar STRs may form a signal cascade. In conclusion, ST and STR are promising properties for quantitative analysis of signal transduction.
Identifying a novel role for the master regulator Tal1 in the Endothelial to Hematopoietic Transition
Authors: Serina Secanechia Yasmin Natalia , Bergiers Isabelle , Rogon Matt , Arnold Christian , Descostes Nicolas , Le Stephanie , López-Anguita Natalia , Ganter Kerstin , Kapsali Chrysi , Bouilleau Lea , Gut Aaron , Uzuotaite Auguste , Aliyeva Ayshan , Zaugg Judith B. , Lancrin Christophe 
Published: October 2022

AbstractProgress in the generation of Hematopoietic Stem and Progenitor Cells (HSPCs) in vitro and ex vivo has been built on the knowledge of developmental hematopoiesis, underscoring the importance of understanding this process. HSPCs emerge within the embryonic vasculature through an Endothelial-to-Hematopoietic Transition (EHT). The transcriptional regulatorTal1exerts essential functions in the earliest stages of blood development, but is considered dispensable for the EHT. Nevertheless,Tal1is expressed with its binding partnerLmo2and it homologousLyl1in endothelial and transitioning cells at the time of EHT. Here, we investigated the function of these genes using a mouse embryonic-stem cell (mESC)-based differentiation system to model hematopoietic development. We showed for the first time that the expression of TAL1 in endothelial cells is crucial to ensure the efficiency of the EHT process and a sustained hematopoietic output. Our findings uncover an important function ofTal1during the EHT, thus filling the current gap in the knowledge of the role of this master gene throughout the whole process of hematopoietic development.
Deconstructing the driving factors of land development intensity from multi-scale in differentiated functional zones
Authors: Yin Yue , Zhou Kan , Chen Yufan 
Published: October 2022

AbstractLand development intensity (LDI) is an important indicator of how much human exploitation of land resources. Against the background of rapid urbanization and industrialization, in order to curb the over-occupation of agricultural and ecological space by urbanized space, China has proposed the Major Functional Zoning Planning (MFZP) as a new spatial governance model, which attempts to implement differentiated LDI control according to the functional positioning of different regions. To observe the spatio-temporal evolution and drivers of LDI in the first five years since the implementation of the MFZP, we chose the Yangtze River Delta, the most urbanized region in China, as a case area. The multi-scale distribution pattern of LDI was analyzed by combining macro and micro scales, and a new spatial measurement method integrating global and local regression models was developed to quantitatively deconstruct the natural-human drivers of LDI in different functional zones. Results demonstrate that LDI decreases in a gradient of the urbanized zone (UZ) > main agricultural production zone (MAPZ) > key ecological function zone (KEFZ). In the UZ, LDI is influenced by natural-human multi factors, e.g., terrain conditions (SLP), proportion of cultivated land area (PCL), proportion of ecologically important area (EID), population urbanization rate (PUR), GDP per capita (PGDP), fixed asset investment size per land (FAI) and fiscal expenditure as a percentage of GDP (FD). The LDI in the MAPZ is mainly affected by the facilitating role of PCL, EID, FAI, and the prominent role of FD, while that in the KEFZ is mainly inhibited by SLP, EID, and driven by the PGDP. The diverse drivers of LDI in different functional zones remind us to implement differentiated spatial control according to functional positioning and to adopt refined policy tools of zoning and classification to ensure that land resources within each type of functional zone can be used sustainably.
Feasibility of ApoC1 serum levels as tumor biomarker in glioblastoma patients: a pilot study
Authors: Hilbert Michelle , Kuzman Peter , Mueller Wolf C. , Meixensberger Jürgen , Nestler Ulf 
Published: October 2022

AbstractApolipoprotein C1 (ApoC1) has been detected immunohistochemically in glioblastoma tissue, probably expressed by activated monocytes and microglia. The present study was conceived to determine whether the amount of intratumoral ApoC1 expression leads to measurable changes of serum levels after glioblastoma resection or during recurrence. 176 blood samples from 70 glioblastoma patients were collected perioperatively and during subsequent therapy. ApoC1 serum levels were determined using an enzyme linked immunosorbent assay (ELISA). High absorption values due to lipemic or hemolytic serum were removed from the final dataset using a stem and leaf plot. Samples were grouped according to the treatment stage to compare mean ApoC1 serum levels. The number of patients with falling or increasing perioperative values was assessed. 167 ApoC1 serum values from 68 glioblastoma patients were amenable to statistical evaluation. Mean ApoC1 serum level was 91.9 µg/ml (n = 167, sd = 36.0). In samples from patients undergoing first glioblastoma resection, the mean preoperative value was significantly higher (94.8 µg/ml, n = 37, sd = 29.5) than after surgery (77.4 µg/ml, n = 41, sd = 23.2, p = 0.009). Individually, falling ApoC1 levels were detected in 25 and rising levels in 9 patients (p = 0.0061). Single absolute serum levels of ApoC1 do not allow an estimation of glioblastoma activity or tumor response. Although pathophysiologically of interest, ApoC1 serum levels did not qualify as a potential biomarker in glioblastoma management. Our results do not seem to encourage larger, multicenter studies.
Efficacy of COVID-HIGIV in animal models of SARS-CoV-2 infection
Authors: Jha Aruni , Barker Douglas , Lew Jocelyne , Manoharan Vinoth , van Kessel Jill , Haupt Robert , Toth Derek , Frieman Matthew , Falzarano Darryl , Kodihalli Shantha 
Published: October 2022

AbstractIn late 2019 the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus emerged in China and quickly spread into a worldwide pandemic. It has caused millions of hospitalizations and deaths, despite the use of COVID-19 vaccines. Convalescent plasma and monoclonal antibodies emerged as major therapeutic options for treatment of COVID-19. We have developed an anti-SARS-CoV-2 immunoglobulin intravenous (Human) (COVID-HIGIV), a potential improvement from using convalescent plasma. In this report the efficacy of COVID-HIGIV was evaluated in hamster and mouse models of SARS-CoV-2 infection. COVID-HIGIV treatment in both mice and hamsters significantly reduced the viral load in the lungs. Among COVID-HIGIV treated animals, infection-related body weight loss was reduced and the animals regained their baseline body weight faster than the PBS controls. In hamsters, COVID-HIGIV treatment reduced infection-associated lung pathology including lung inflammation, and pneumocyte hypertrophy in the lungs. These results support ongoing trials for outpatient treatment with COVID-HIGIV for safety and efficacy evaluation (NCT04910269, NCT04546581).
Metagenomic DNA sequencing to quantify Mycobacterium tuberculosis DNA and diagnose tuberculosis
Authors: Chang Adrienne , Mzava Omary , Djomnang Liz-Audrey Kounatse , Lenz Joan Sesing , Burnham Philip , Kaplinsky Peter , Andama Alfred , Connelly John , Bachman Christine M. , Cattamanchi Adithya , Steadman Amy , De Vlaminck Iwijn 
Published: October 2022

AbstractTuberculosis (TB) remains a significant cause of mortality worldwide. Metagenomic next-generation sequencing has the potential to reveal biomarkers of active disease, identify coinfection, and improve detection for sputum-scarce or culture-negative cases. We conducted a large-scale comparative study of 428 plasma, urine, and oral swab samples from 334 individuals from TB endemic and non-endemic regions to evaluate the utility of a shotgun metagenomic DNA sequencing assay for tuberculosis diagnosis. We found that the composition of the control population had a strong impact on the measured performance of the diagnostic test: the use of a control population composed of individuals from a TB non-endemic region led to a test with nearly 100% specificity and sensitivity, whereas a control group composed of individuals from TB endemic regions exhibited a high background of nontuberculous mycobacterial DNA, limiting the diagnostic performance of the test. Using mathematical modeling and quantitative comparisons to matched qPCR data, we found that the burden of Mycobacterium tuberculosis DNA constitutes a very small fraction (0.04 or less) of the total abundance of DNA originating from mycobacteria in samples from TB endemic regions. Our findings suggest that the utility of a minimally invasive metagenomic sequencing assay for pulmonary tuberculosis diagnostics is limited by the low burden of M. tuberculosis and an overwhelming biological background of nontuberculous mycobacterial DNA.
Role of income and government responsiveness in reducing the death toll from floods in Indian states
Authors: Parida Yashobanta , Roy Chowdhury Joyita , Saini Swati , Dash Devi Prasad 
Published: October 2022

AbstractFloods are the most commonly occurring natural disasters in India due to India’s unique geographical location and socioeconomic conditions. Frequent flooding causes enormous loss of human lives and damage crops and public utilities. Furthermore, floods adversely affect economic development and increase the government's financial burden by increasing spending on various disaster mitigation measures. Recent empirical literature based on cross-national comparisons shows that disaster fatalities and damages are monotonically decreasing in per capita income. We challenge this view on the monotonic negative relationship between income and flood damages. We examine the non-monotonic (inverted U-shaped) relationship between per capita income and flood impact in terms of deaths, people affected, and damages due to floods in 19 major Indian states from 1980 to 2011, using Poisson and Tobit estimation methods. In particular, deaths and the population affected by floods increase with a turning point of income up to 882 US$ and 578 US$, respectively, and diminishes thereafter. Our results confirm an inverted U-shaped relationship between income and fatalities and the population affected by floods. In addition to income, we argue that government responsiveness plays an essential role in mitigating the risk of floods. We employ the fixed-effect Poisson estimation method to examine the government's role in protecting people against disaster risk, focusing on regional differences in India. Deaths from floods remain non-linear and follow the inverted U-pattern with respect to government responsiveness. However, the effect of government responsiveness on flood fatalities and flood damages is statistically insignificant. Our results further suggest that high-income states experience a lower death toll from floods. The high-income (rich) states are capable of incurring a higher threshold level of income and higher natural calamity expenditure to reduce flood fatalities and protect the population affected by floods than the low-income (poor) states. The poor states have minimal resources and face severe financial constraints to reduce the death toll from floods. From the perspective of public policy, the poor states, in particular, require an increase in income, better governance, and effective disaster management policies to mitigate flood impact.