bluuebunny/crossref_metadata_2025_split huggingface dataset:
Highly-multiplexed serology for non-human mammals
Authors: Schuettenberg Alexa , Piña Alejandra , Metrailer Morgan , Peláez-Sánchez Ronald Guillermo , Agudelo-Flórez Piedad , Álvaro Lopez Juan , Ryle Luke , Monroy Fernando P. , Altin John A. , Ladner Jason T. 
Published: May 2022

AbstractEmerging infectious diseases represent a serious and ongoing threat to humans. Most emerging viruses are maintained in stable relationships with other species of animals, and emergence within the human population results from cross-species transmission. Therefore, if we want to be prepared for the next emerging virus, we need to broadly characterize the diversity and ecology of viruses currently infecting other animals (i.e., the animal virosphere). High-throughput metagenomic sequencing has accelerated the pace of virus discovery. However, molecular assays can only detect active viral infections and only if virus is present within the sampled fluid or tissue at the time of collection. In contrast, serological assays measure long-lived antibody responses to infections, which can be detected within the blood, regardless of the infected tissues. Therefore, serological assays can provide a complementary approach to understanding the circulation of viruses, and while serological assays have historically been limited in scope, recent advancements allow 1000s to 100,000s of antigens to be assessed simultaneously using <1 μl of blood (i.e., highly-multiplexed serology). Application of highly-multiplexed serology for characterization of the animal virosphere is dependent on the availability of reagents that can be used to capture or label antibodies of interest. Here, we demonstrate the potential for commercial immunoglobulin-binding proteins (protein A and protein G) to enable highly-multiplexed serology in 25 species of non-human mammals and we describe a competitive FLISA assay that can be used as an initial screen for choosing the most appropriate capture protein for a given host species.ImportanceAntibodies are generated in response to infections with viruses and other pathogens and they help protect against future exposures. Mature antibodies are long-lived, highly specific, and can bind to their protein targets with high affinity. Thus, antibodies can also provide information about an individual’s history of viral exposures, which has important applications in understanding the epidemiology and etiology of disease. In recent years, there have been large advances in the available methods for broadly characterizing antibody binding profiles, but thus far, these have primarily been utilized only with human samples. Here, we demonstrate that commercial antibody-binding reagents can facilitate modern antibody assays for a wide variety of mammalian species, and we describe a cheap and fast approach for choosing the best reagent for each animal species. By studying antibody-binding profiles in captive and wild animals, we can better understand the distribution and prevalence of viruses that could spillover into humans.
A quantitative metric of pioneer activity reveals that HNF4A has stronger in vivo pioneer activity than FOXA1
Authors: Hansen Jeffrey L , Cohen Barak A 
Published: May 2022

AbstractWe and others have suggested that pioneer activity–a transcription factor’s (TF’s) ability to bind and open inaccessible loci–is not a qualitative trait limited to a select class of pioneer TFs. We hypothesize that most TFs display pioneering activity that depends on the TF concentration and the motif content at their target loci. Here we present a quantitative measure of pioneer activity that captures the relative difference in a TF’s ability to bind accessible versus inaccessible DNA. The metric is based on experiments that use CUT&Tag to measure binding of doxycycline (dox) inducible TFs. For each location across the genome we determine a “dox50,” the concentration of dox required for a TF to reach half-maximal occupancy. We propose that the ratio of a TF’s average dox50 between ATAC-seq labeled inaccessible and accessible binding sites, its Δdox50, is a measure of its pioneer activity. We measured Δdox50’s for the endodermal TFs FOXA1 and HNF4A and show that HNF4A has a smaller Δdox50 than FOXA1, suggesting that HNF4A has stronger pioneer activity than FOXA1. We further show that FOXA1 binding sites with more copies of its motif have a lower Δdox50, suggesting that strong motif content may compensate for weak pioneer activity. The quantitative analysis of binding suggests different modes of binding for FOXA1, including an anti-cooperative mode of binding at certain accessible loci. Our results suggest that Δdox50s, or other similar measures that assess the difference in TF affinity for inaccessible and accessible DNA, are reasonable measures of pioneer activity.
Input-Specific Inhibitory Plasticity Improves Decision Accuracy Under Noise
Authors: Song Soomin C. , Shen Bo , Machold Robert , Rudy Bernardo , Glimcher Paul W. , Louie Kenway , Froemke Robert C. 
Published: May 2022

SummaryInhibitory interneurons regulate excitability, information flow, and plasticity in neural circuits. Inhibitory synapses are also plastic and can be modified by changes in experience or activity, often together with changes to excitatory synapses. However, given the diversity of inhibitory cell types within the cerebral cortex, it is unclear if plasticity is similar for various inhibitory inputs or what the functional significance of inhibitory plasticity might be. Here we examined spike-timing-dependent plasticity of inhibitory synapses from four major subtypes of GABAergic cells onto layer 2/3 pyramidal cells in mouse auditory cortex. The likelihood of inhibitory potentiation varied across cell types, with somatostatin-positive (SST+) interneuron inputs exhibiting the most potentiation on average. A network simulation of perceptual decision-making revealed that plasticity of SST+-like inputs provided robustness from higher input noise levels to maintain decision accuracy. Differential plasticity at specific inhibitory inputs therefore may be important for network function and sensory perception.
Intranasal vaccination induced cross-protective secretory IgA antibodies against SARS-CoV-2 variants with reducing the potential risk of lung eosinophilic immunopathology
Authors: Hemmi Takuya , Ainai Akira , Hashiguchi Takao , Tobiume Minoru , Kanno Takayuki , Iwata-Yoshikawa Naoko , Iida Shun , Sato Yuko , Miyamoto Sho , Ueno Akira , Sano Kaori , Saito Shinji , Shiwa-Sudo Nozomi , Nagata Noriyo , Tamura Koji , Suzuki Ryosuke , Hasegawa Hideki , Suzuki Tadaki 
Published: May 2022

AbstractTo control the coronavirus disease 2019 (COVID-19) pandemic, there is a need to develop vaccines to prevent infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. One candidate is a nasal vaccine capable of inducing secretory IgA antibodies in the mucosa of the upper respiratory tract, the initial site of infection. However, regarding the development of COVID-19 vaccines, there is concern about the potential risk of inducing lung eosinophilic immunopathology as a vaccine-associated enhanced respiratory disease as a result of the T helper 2 (Th2)-dominant adaptive immune response. In this study, we investigated the protective effect against virus infection induced by intranasal vaccination of recombinant trimeric spike protein derived from SARS-CoV-2 adjuvanted with CpG oligonucleotides, ODN2006, in mouse model. The intranasal vaccine combined with ODN2006 successfully induced not only systemic spike-specific IgG antibodies, but also secretory IgA antibodies in the nasal mucosa. Secretory IgA antibodies showed high protective ability against SARS-CoV-2 variants (Alpha, Beta and Gamma variants) compared to IgG antibodies in the serum. The nasal vaccine of this formulation induced a high number of IFN-γ-secreting cells in the draining cervical lymph nodes and a lower spike-specific IgG1/IgG2a ratio compared to that of subcutaneous vaccination with alum as a typical Th2 adjuvant. These features are consistent with the induction of the Th1 adaptive immune response. In addition, mice intranasally vaccinated with ODN2006 showed less lung eosinophilic immunopathology after viral challenge than mice subcutaneously vaccinated with alum adjuvant. Our findings indicate that intranasal vaccine adjuvanted with ODN2006 could be a candidate that can prevent the infection of antigenically different variant viruses, reducing the risk of vaccine-associated enhanced respiratory disease.
A car wash: post-truth politics, Petrobras and ethics of the real
Authors: Voss Barbara d.L. , Carter David B. , Warren Rebecca 
Published: May 2022

PurposeThe study draws upon three accounts to examine post-truth politics and its link to accounting. In studying Petrobras, a Brazilian petrochemical company embroiled in a corruption scandal, the authors draw upon a politics of falsity to understand how different depictions of similar events can emerge. The authors depict Petrobras' corporate social responsibility (CSR) disclosures during the period of corruption juxtaposed against the Brazilian Federal Police investigation (the Lava Jato/Car Wash Operation) and Petrobras' response to the allegations of institutional corruption.Design/methodology/approachThe data set consisted of 56 Petrobras reports including Annual Reports, Financial Statements, Sustainability Reports and Form 20-Fs from 2004 to 2017, information disclosed by the Brazilian Federal Police concerning the Lava Jato Operation and media reports concerning Petrobras and the corruption scandal. The paper employs a discourse analysis approach to depict and interpret the accounts.FindingsThrough examining the connection between ontic accounts and ontological presuppositions, the authors illustrate a post-truth logic underpinning accounting, due to the interpretive, contestable and contingent nature of accounting information. Consequently, the authors turn to the “ethics of the real” as a response, as citizen subjects must be cautious in how they approach accounting and CSR disclosures.Originality/valueRather than relying on simplistic true/false dualities, the authors argue that the “ethics of the real” provides a courageous position for citizen subjects to interrogate the organisation by recognising the role of discourse and disclosure expectations on organisations in a post-truth environment. The study also illustrates how competing, contingent accounts of the same timeframe and events can emerge.
Elucidating corporate governance’s impact and role in countering fraud
Authors: Kassem Rasha 
Published: May 2022

Purpose
This paper aims to highlight the role and impact of corporate governance in combating fraud by drawing on insights from the literature, identify gaps in the literature and suggest new directions for future research.

Design/methodology/approach
The paper is based on a comprehensive general literature review using multiple search engines and databases.

Findings
This paper finds that effective corporate governance can help reduce fraud risk, prevent fraud and detect fraud, particularly corporate fraud, insider fraud and asset diversion. Some companies use corporate governance mechanisms to bolster their reputation following fraud detection. Ineffective corporate governance increases fraud risk, provides the opportunity for perpetrating fraud and reduces the likelihood of fraud detection. The paper sheds light on several governance mechanisms that could help in mitigating fraud risk, as reported in the literature. The paper categorises these governance mechanisms into four broad governance aspects, including board leadership and the role of ethics; (b) board characteristics, composition and structure; ownership structure; accountability. The paper proposes a guide summarising these broad fundamental governance aspects, including specific anti-fraud controls and examples of how organisations could enhance ethical cultures and the tone at the top.

Originality/value
To the best of the author’s knowledge, this is the first paper to elucidate the role of corporate governance in countering fraud and develop guidance in this area. The proposed guidance could be helpful to businesses leaders, policymakers, researchers and academics alike.
Executive shareholding, institutional investor shareholding and enterprise innovation
Authors: Wang Tai , Cheng Daoping 
Published: May 2022

PurposeThe purpose of this study is to empirically investigate the relationship between executive shareholding, institutional investor shareholding and corporate innovation, and to further explore in depth the impact of executive shareholding on corporate innovation under different industries.Design/methodology/approachThis paper uses the panel data of A-share listed companies in Shanghai and Shenzhen from 2012 to 2020 as the research sample to empirically study the relationship between executive shareholding, institutional investor shareholding and corporate innovation based on multiple linear regression models and panel fixed effects.FindingsThe research shows that: on the whole, the impact of executive shareholding on enterprise innovation presents an inverted “U” shape; institutional investors will negatively regulate the impact of executive shareholding on enterprise innovation; the impact of executive shareholding on enterprise innovation will show obvious industry differences in different industries.Research limitations/implicationsThe empirical results not only enrich the research on the effects of institutional investors' involvement in corporate governance practice, but also provide targeted experience for promoting enterprise innovation. Due to the limitations of innovation indicators and industry sample selection, it is necessary to be cautious when extending the results to other fields.Practical implicationsEnterprises should fully consider the impact of executive shareholding on innovation and formulate a scientific executive incentive system according to the differences of their industries. The government should be aware of the important role of institutional investors in enterprises, improve the channels and ways for institutional investors to participate in corporate governance, and improve the basic system of capital markets.Originality/valueOn the one hand, this paper empirically tests the regulatory role of institutional investors' shareholding and the relationship between executive shareholding and enterprise innovation, which enriches the research on the effect of institutional investors' involvement in corporate governance practice. On the other hand, the research by industry is more targeted to provide experience for promoting enterprise innovation.
Will Rota 2030 deliver sustainable innovation? Comparing the ends and means of Brazilian and German auto industry innovation policies
Authors: Pelegrina Juliano , Stoeber Timo , Fouto Nuno Manoel Martins Dias 
Published: May 2022

Purpose Due to dramatic transformation of the auto industry, governments are implementing innovation policies to ensure the domain of sustainable technologies. According to the literature, developing countries that depend on multinational subsidiaries must invest in complementary innovation to be part of their research and development (R&D) headquarters' long-term plans. This study analyses the Brazilian auto industry innovation policy (Rota 2030) to evaluate if it targets complementarity with the German's one (NPE). It also compares the institutional arrangements of the former against the latter to check for governance gaps.Design/methodology/approachIt applies a case-oriented comparative method (Ragin, 2014) for the analysis of qualitative evidence on secondary data. It investigates evidence of complementarity between Rota 2030 and national platform for electric mobility (NPE) objectives and checks for governance gaps in Rota 2030 using NPE as a reference.FindingsThe results confirmed a loose fitting between the innovation policies mainly for a lack of determinism of Rota 2030 objectives. Governance gaps were also found on Rota 2030 policy formulation and operationalization.Practical implicationsIt contributes for the improvement of Rota 2030, and its analytical frame may be used for the formulation or adjustment of other developing countries' innovation policies.Originality/valueIt contributes with innovation system and policy field development with a theoretical extension coming from the New Institutional Economics (NIE) (Menard, 2018). By examining the performance of “institutional arrangements” during the process of formulation and operationalization of innovation policies, it shows the importance of coordination for their effectiveness.
A LAG-based framework to overcome the challenges of the sustainable vaccine supply chain: an integrated BWM–MARCOS approach
Authors: Yadav Amit Kumar , Kumar Dinesh 
Published: May 2022

PurposeEach individual needs to be vaccinated to control the spread of the COVID-19 pandemic in the shortest possible time. However, the vaccine distribution with an already strained supply chain in low- and middle-income countries (LMICs) will not be effective enough to vaccinate all the population in stipulated time. The purpose of this paper is to show that there is a need to revolutionize the vaccine supply chain (VSC) by overcoming the challenges of sustainable vaccine distribution.Design/methodology/approachAn integrated lean, agile and green (LAG) framework is proposed to overcome the challenges of the sustainable vaccine supply chain (SVSC). A hybrid best worst method (BWM)–Measurement of Alternatives and Ranking According to COmpromise Solution (MARCOS) methodology is designed to analyze the challenges and solutions.FindingsThe analysis shows that vaccine wastage is the most critical challenge for SVSC, and the coordination among stakeholders is the most significant solution followed by effective management support.Social implicationsThe result of the analysis can help the health care organizations (HCOs) to manage the VSC. The effective vaccination in stipulated time will help control the further spread of the virus, which will result in the normalcy of business and availability of livelihood for millions of people.Originality/valueTo the best of the author's knowledge, this is the first study to explore sustainability in VSC by considering the environmental and social impact of vaccination. The LAG-based framework is also a new approach in VSC to find the solution for existing challenges.
How incentive synergy and organizational structures shape innovation ambidexterity
Authors: Zhang Qingqiang , Sun Xinbo 
Published: May 2022

Purpose
Organizational incentives and structures play a crucial role in realizing explorative and exploitative innovations in firms. Existing studies have neglected the role of trade-off mechanisms between the two on innovation ambidexterity. This study aims to investigate these trade-off mechanisms and their position on innovation ambidexterity.

Design/methodology/approach
Given the limited theoretical understanding, the authors conducted a case study with a sample of two Chinese firms with abundant interview and secondary data.

Findings
The results show that firms can develop innovation ambidexterity at two levels, namely, the time and space levels, using incentive synergy as well as organizational structures. Furthermore, the authors explain the role of the trade-off between incentive synergy and organizational structure in promoting a balance between explorative and exploitative innovation.

Originality/value
The authors propose trade-off mechanisms between incentive synergy and organizational structure and explore how trade-off mechanisms can play a role in promoting a balance of explorative and exploitative innovation at both time and space levels.
Designing synchronizer module in CMMS software based on lean smart maintenance and process mining
Authors: Hosseinizadeh Mazloumi Seyed Hesam , Moini Alireza , Agha Mohammad Ali Kermani Mehrdad 
Published: May 2022

PurposeNew maintenance hypotheses such as lean smart maintenance emphasized internal integration. Since the maintenance process is not fully integrated with other business processes, it indicates that some of the problems in the maintenance process are caused by other departments. Additionally, nothing can be managed or improved without first measuring it. In order to enhance internal integration, this study developed a model that makes use of information systems data to examine synchronization and collaboration across departments engaged in maintenance operations.Design/methodology/approachThis research connects maintenance management and business process management through information systems. A conceptual module model based on CMMS is proposed that will use data which are already available in CMMS and, using process mining, will assess the level of synchronization between departments within an organization.FindingsThis conceptual model will serve as a roadmap for creating better value-added CMMS software. This system operates as a performance measurement tool in three majors, including organizational analysis, workflow analysis and eventually, a future simulation of maintenance processes. This module will serve as a decision support system, highlighting opportunities for improvement in maintenance processes.Originality/valueA practical guideline is provided for the future development of CMMSs and their enhancement to intelligence. All assumptions are based on maintenance theories, techniques for measuring maintenance performance and business process management and process mining.
Finding good cops: the foundations of a screen-in (not out) hiring process for police
Authors: Terpstra Brice , White Michael D. , Fradella Henry F. 
Published: May 2022

PurposeThe purpose of this study is to outline components of a proposed screen-in (not out) hiring process for police.Design/methodology/approachThis study uses survey data collected from police officers of all ranks (n = 166) in one Arizona police department, exploring officer perceptions of the most important qualities necessary to be an exceptional police officer, as well as the optimal processes for finding people with those traits.FindingsResults build on prior studies that have begun to identify sought-after traits including honesty, integrity, ethics, compassion, empathy, as well as communication and listening skills. Findings also suggest preliminary steps for finding good cops through expansion of existing screen-out processes to also screen-in desirable prospects, application of some academy training processes to the hiring pool, and more proactive recruitment efforts.Originality/valueThis study adds value to the extant literature on the characteristics of good policing and on identifying the qualities by which to “screen-in” officers during the hiring and recruitment process. Further, the authors expand this understanding by capturing perceptions of the best processes to identify individuals with desirable qualities during the hiring and recruitment process. The implications of these findings are used to initiate a discussion of foundational components of a screening-in hiring process for police, informed by both empirical research and community perspectives.