bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three novel marine species of the genus Fulvivirga, rich sources of carbohydrate-active enzymes degrading polysaccharides
Authors: Nguyen Tra T. H. , Vuong Tien Q. , Han Ho Le , Li Zhun , Lee Yong-Jae , Ko Jaeho , Nedashkovskaya Olga I. , Kim Song-Gun 
Published: October 2022

Abstract
Bacteroidota is a group of marine polysaccharide degraders, which play a crucial role in the carbon cycle in the marine ecosystem. In this study, three novel gliding strains, designated as SS9-22T, W9P-11T, and SW1-E11T, isolated from algae and decaying wood were proposed to represent three novel species of the genus Fulvivirga. We identified a large number of genes encoding for carbohydrate-active enzymes, which potentially participate in polysaccharide degradation, based on whole genome sequencing. The 16S rRNA sequence similarities among them were 94.4–97.2%, and against existing species in the genus Fulvivirga 93.1–99.8%. The complete genomes of strains SS9-22T, W9P-11T, and SW1-E11T comprised one circular chromosome with size of 6.98, 6.52, and 6.39 Mb, respectively; the GC contents were 41.9%, 39.0%, and 38.1%, respectively. The average nucleotide identity and the digital DNA-DNA hybridization values with members in the genus Fulvivirga including the isolates were in a range of 68.9–85.4% and 17.1–29.7%, respectively, which are low for the proposal of novel species. Genomic mining in three genomes identified hundreds of carbohydrate-active enzymes (CAZymes) covering up to 93 CAZyme families and 58–70 CAZyme gene clusters, exceeding the numbers of genes present in the other species of the genus Fulvivirga. Polysaccharide-degrading activities were found, highlighting that the three strains are rich sources of CAZymes of polysaccharide degraders for biotechnological applications. The phenotypic, biochemical, chemotaxonomic, and genomic characteristics supported the proposal of three novel species in the genus Fulvivirga, for which the names Fulvivirga ulvae sp. nov. (SS9-22T = KCTC 82072T = GDMCC 1.2804T), Fulvivirga ligni sp. nov. (W9P-11T = KCTC 72992T = GDMCC 1.2803T), and Fulvivirga maritima sp. nov. (SW1-E11T = KCTC 72832T = GDMCC 1.2802T) are proposed.
Baseline Characteristics of the Korean Genetic Cohort of Inherited Cystic Kidney Disease
Authors: Cho Jeong Min , Park Hayne Cho , Lee Jin Woo , Ryu Hyunjin , Kim Yong Chul , Ahn Curie , Lee Kyu-Beck , Kim Yeong Hoon , Han Seungyeup , Kim Yaerim , Bae Eun hui , Kang Hee Gyung , Park Eujin , Jeong Kyungjo , Kang Seoon , Choi Jungmin , Oh Kook-Hwan , Oh Yun Kyu 
Published: October 2022

Abstract
Introduction: Identification of inherited cystic kidney disease genes in each case is necessary for precise treatment.
Methods: This is a 3-year prospective, multicenter cohort study including patients with more than three renal cysts from May 2019 to May 2022. Clinical and genetic characteristics were evaluated.
Results: A total of 725 adult patients were enrolled. Mean age was 46.2 ± 14.0 years, and 48.6% were male. Patients were categorized into typical ADPKD (560, 77.2%) and atypical PKD (165, 22.8%). Typical ADPKD based on Mayo imaging classification (MIC) I were classified as follows: (1) 1A 55 (9.9%), (2) 1B 149 (26.9%), (3) 1C 198 (35.8%), (4) 1D 90 (16.3%), and (5) 1E 61 (11.0%). Atypical PKD by MIC II included bilateral cystic with bilateral atrophic (31, 37.3%), lopsided (27, 32.5%), unilateral (9, 10.8%), segmental (8, 9.6%), bilateral cystic with unilateral atrophic (7, 8.4%), and asymmetric (1, 1.2%). Pathogenic variants were found in 57.3% of the patients using the ciliopathy-related gene panel. The typical ADPKD group demonstrated a higher discovery rate (62.3%) compared to atypical PKD group (41.8%).
Conclusion: We report the baseline and genetic characteristics of the nationwide genetic cohort for Korean hereditary cystic kidney disease prior to detailed molecular analysis.
Comparative study on high-pressure physical properties of monoclinic MgCO3 and Mg2CO4
Authors: Liu Zi-Jiang , Li Tian , Sun Xiao-Wei , Zhang Cai-Rong , Ju Jia-Qi 
Published: October 2022

Abstract
The physical properties of Mg-carbonate at high temperatures and pressure are crucial for understanding the deep carbon cycle. Here, we use first-principles calculations to study the physical properties of MgCO3-C2/m and Mg2CO4-P21/c under high pressure. The research shows that the structure and the equation of state of MgCO3-C2/m are in good agreement with the experimental results, and the phase transition pressure of Mg2CO4 from pnma to P21/c is 44.66 GPa. By comparing the elastic properties, seismic properties, and anisotropy of MgCO3-C2/m and Mg2CO4-P21/c, it is found that the elastic modulus and sound velocity of Mg2CO4-P21/c are smaller than those of MgCO3-C2/m, while the anisotropy is larger than that of MgCO3-C2/m. These results indicate that Mg2CO4-P21/c exists in the deep mantle and may be the main reason why carbonate cannot be detected. The minimum thermal conductivity of MgCO3-C2/m and Mg2CO4-P21/c is the largest in the [010] direction and the smallest in the [001] direction. The thermodynamic properties of MgCO3-C2/m and Mg2CO4-P21/c are predicted using the quasi-harmonic approximation (QHA) method.
Targeting EphA2 and DDR signaling can overcome the BRAF and MEK inhibitors acquired resistance in melanoma cell lines
Authors: Belli Valentina , Napolitano Stefania , De Falco Vincenzo , Suarato Gabriella , Perrone Alessandra , Guerrera Luigi Pio , Martini Giulia , Corte Carminia Maria Della , Martinelli Erika , Morgillo Floriana , Turano Mimmo , Furia Maria , Argenziano Giuseppe , Ciardiello Davide , Ciardiello Fortunato , Troiani Teresa 
Published: October 2022

Abstract

The BRAF and MEK inhibitors combined strategies have dramatically changed the outcome of BRAF-mutated metastatic melanoma patients. However, despite the initial promising results, the onset of primary or acquired resistance occurs in nearly half of the patients at about 1 year from the diagnosis. Understanding the mechanisms of resistance to these inhibitors is therefore critical for planning more effective therapeutic strategies able to improve patient outcomes. To this aim we generated BRAF and MEK inhibitors resistant melanoma cells starting from the SAN and A375 lines, both harboring the most common BRAF-V600 mutation and sensitive to these drugs. The obtained double-resistant cell lines were characterized by MTT cell proliferation, migration, invasion assays, phosphoarray and western blot analysis. Here we report that the overexpression of several Tyrosine Kinase Receptors (TKRs), such as EphA2 and DDRs, drives the resistance to these drugs and that this resistance can be overcome by treatment with ALW-II-27-41 multikinase inhibitor. ALW-II-27-41 blocks not only TKRs expression, but also the related downstream AKT and MAPK signaling pathways and its efficacy is documented by decreased cell viability and reduced cell invasion/migration of the resistant cells. Our results can delineate a novel promising therapeutic approach to overcoming the drug resistance occurring in BRAF-mutated metastatic melanoma.
Prediction of Organic Compound Aqueous Solubility Using Interpretable Machine Learning- A Comparison Study of Descriptor-Based and Topological Models
Authors: Tayyebi Arash , Alshami Ali , Rabiei Zeinab , Yu Xue , Ismail Nadhem , Talukder Musabbir J , Power Jason 
Published: October 2022

Abstract
A reliable and practical determination of a chemical species’ solubility in water continues to be examined using empirical observations and exhaustive experimental studies alone. Predictions of chemical solubility in water using data-driven algorithms can allow us to create a rationally designed, efficient, and cost-effective tool for next-generation materials and chemical formulations. We present results from two machine learning (ML) modeling studies to adequately predict various species’ solubility using data for over 8,400 compounds. Molecular-descriptors, the most used method in previous studies, and Morgan fingerprint, a topological, circular-based hash of the molecules' structures, were applied to produce water solubility estimates. We trained all models on 80% of the total datasets using the Random Forest (RFs) technique as the regressor and tested the prediction performance using the remaining 20%, resulting in R2 test values of 0.88 and 0.82 for the descriptors and circular fingerprint methods, respectively. We interpreted the produced ML models and reported the most effective features for aqueous solubility measures using Shapley Additive exPlanations (SHAP) and thermodynamic analysis. Low error, ability to investigate the molecular-level interactions and compatible with thermodynamic quantities made fingerprint a distinct model compared to other available computational tools.
CRYAB exerts anti-tumor effect as a favorable prognostic factor in prostate cancer
Authors: Zhang Chunlei , Hu You , Wang Dongxing , Kang Yindong , Yang Qi , Chang Dehui 
Published: October 2022

Abstract
Background: CRYAB is closely related to apoptosis, invasion and migration of tumor cells. Nevertheless, the relationship between CRYAB, outcomes of prostate cancer (PCa), and the potential mechanisms have not been studied.
Material and Methods: The TPM format RNA sequencing (RNAseq) and methylation data on differential expression came from the cancer genome atlas (TCGA) database. R software and packages were used to analyze the different expression of CRYAB in TCGA database, the correlation between gene expression and immune cell infiltration, receiver operating characteristic curve, cox regression, Kaplan-Meier curve, nomogram models and calibration curve. Then, the bio-function of CRYAB in prostate cancer cell lines was detected via cell counting kit‑8, invasion and terminal deoxynucleotidyl transferase dUTP nick end labelling assay.
Results: Compared to normal tissues, CRYAB was down-regulated in prostate cancer tissues based on the TCGA database, and was corrected with Gleason score. The promoter methylation level of CRYAB in PCa was significantly higher than that in normal. Immune cell infiltration analysis showed that the infiltration of all these immune cells except TReg was positively related to CRYAB expression. ROC curve and KM curve demonstrated that CRYAB had a meaningful diagnosis and prognostic value. Cox regression analysis indicated that higher T stage, N stage, PSA, Gleason score, and lower CRYAB expression were prognostic indicators of PCa. Cell function experiments suggested that up-regulated CRYAB can inhibit the proliferation, invasion and migration of prostate cancer cells, and promote apoptosis.
Conclusion:We found that CRYAB was down-expressed in tumor tissues, and was related with anti-tumor function in PCa cells. We identified CRYAB had a significant diagnosis and prognostic value in PCa.
Choriocarcinoma syndrome of primary pulmonary choriocarcinoma after lung lobe wedge resection: A case report and review of the literature
Authors: Chen Chen , Zhao Qian , Li Yi , Liu Zhenjun , Ji Juan , Zhao Pei 
Published: October 2022

Abstract
Introduction: Primary pulmonary choriocarcinoma (PPC) is extremely rare, especially in males. It is characterized by a poor response to therapy and shortened survival times. Here, we report a PPC case that was complicated by choriocarcinoma syndrome (CS) and present a review of the literature.
Case presentation: A 59-year-old man with a 30-pack-year smoking history was referred to our hospital because of haemoptysis and chest pain. Positron emission tomography-computed tomography (PET-CT) showed multiple bilateral pulmonary nodules and multiple metastases throughout the body. The patient underwent single-port thoracoscopic wedge resection of the right lung upper lobe. Histological and β-HCG examinations verified the diagnosis of choriocarcinoma. Three days after the operation, the patient developed massive haemoptysis. A bronchoscopic examination showed bleeding from the bilateral main bronchus. Despite management in the intensive care unit (ICU), the patient died two weeks after surgery.
Conclusions: PPC is highly malignant, progresses rapidly, often exhibits metastases and has a poor prognosis. Treatments such as surgery and chemotherapy may be predisposing factors for CS. Surgery combined with chemotherapy and radiotherapy, based on the physical condition of the patient, may significantly to improve the prognosis of patients.
Pilot study: validity and reliability of textile insoles used to measure the characteristics of landing tasks during rehabilitation and artistic gymnastics
Authors: Schümperlin Delia , Schärer Christoph , Kalberer Luzia , Ferguson Stephen John , Lorenzetti Silvio René 
Published: October 2022

Abstract

Objectives: Artistic gymnastics is a sport where most athletes start at an early age and training volumes are high. Hence, overuse and acute injuries are frequent due to the load endured during landing tasks. During landing, the ground reaction force (GRF) is up to 15.8 times the body weight and therefore reliable GRF measurements are crucial. The gold standard for GRF measurements are force plates. As force plates are mostly used in a constrained laboratory environment, it is difficult to measure the GRF in representative training settings. Textile insoles (novel GmbH, Munich, Germany) exist, which can be used to measure dynamic GRF. Hence, the motivation of this study is to test the validity and reliability of these insoles during landing tasks. GRF was measured during four different exercises, in two test subjects and compared to concurrent force plate data.
Results: Twelve out of 16 statistical parametric mapping plots showed no significant difference between the measured force curves of insoles and force plates. Across conditions, the root mean square error of the maximal vertical GRF was 21N/kg and of impulse 0.4Ns/kg. The intraclass correlation coefficient (ICC 2,1) ranged from 0.02 to 0.76 for maximal vertical GRF and from -0.34 to 0.76 for impulse. Generally, the insoles underestimated the GRF and are only a valid measurement tool when analysing the impulse of a jump.
Estimation of Water Hyacinth Using Computer Vision
Authors: ADAMON Gildas David Farid , KONNON Miton Abel , RAYMOND Merscial , N’DEJI Rodolphe , AGONMAN Aime , GBAGUIDI Adonaï , GUIDI Tognon Clotilde , FAGBEMI Latif Adeniyi 
Published: October 2022

Abstract
methods
empirical relationships between yield and production indices calculated experimentally, structural parameters measured or calculated through specific experiments (not dynamic), etc. These methods lose precision depending on the type of plant, cultural methods and practices and the seasons. Then, it becomes urgent to develop a dynamic estimation method with a proven track record of reliability despite the inconsistency of the factors mentioned above. This article contributes to the improvement of aquatic biomass estimation by proposing a Computer Vision based solution for estimating fresh mass of water hyacinth. To achieve this goal, the morphology of the species is assessed and an XML classifier is developed. This model is then implemented in a mobile app facilitating its end use. The proposed algorithm demonstrated a mean average precision of 96.89%. Considering the recorded level of accurateness, the developed method can be used to estimate different types of biomass.
Effect of colorants on interfacial compatibility in wood flour/Poly (β-hydroxybutyrate valerate) composites
Authors: Feng Xiao , shaojie Huo , Lizhi Zhu , Haiyan Song , Lili Yu , Yanling Xie 
Published: October 2022

Abstract
To investigate the effect of three kinds of colorants on interfacial compatibility between wood and polymer in wood flour/Poly (β-hydroxybutyrate valerate) (PHBV) composites, three colorants are added to color the poplar wood flour/PHBV composites. The effects of the colorant on the interfacial compatibility of the wood plastic composites (WPCs) are studied. The result shows that the mechanical properties of the WPCs tend to increase first and then decrease with increasing colorant. The SEM micrographs of the WPCs show the surface, partially the cracks and grooves of the wood fiber are coated by the dispersed colorant. There are basically no new characteristic peaks in FT-IR spectra, which is proved that three colorants can only enable wood flour to be fully mixed with PHBV. Three colorants can improve the compatibility of wood fiber and PHBV, the mechanical properties of the WPCs, which is significantly increased compared to the WPCs without colorants.
Study of pulmonary fibrosis as a complication in COVID-19
Authors: Rasheed Modar Haj , Alahmadi Qusay , Shihabi Farah , albardan Husam 
Published: October 2022

Abstract
Background
Post covid pulmonary fibrosis is a seious complication that occurs in covid-19 patients where the normal lung tissue is replaced by a fibrous one due to long periods of disease. Admission into the ICU and severity of disease are most associated with development of post covid pulmonary fibrosis. We plan on investigating the most common risk factors in patients admitting to Damascus hospital
Methods
A cross sectional study involving 314 patients who were admitted to Damascus hospital in the periods between july 2020 to december 2021
Results
There was a statistically significant relationship between the severity of the disease and onset of fibrosis (p < 0.05). Regarding demographic variables such as age and sex we were unable to find any association with these factors
Conclusion
In order to prevent fibrosis in survivors, it is of utmost importance to provide clinical care especially those that have been admitted with severe manifestations or had chronic disease as they were at a risk of developing pulmonary fibrosis in the future.
‘Asking to use a condom with your wife is an admission of cheating while you were away on your fishing expedition’: HIV vulnerability and condom use among fishers at Elmina fishing community in Ghana
Authors: Kyei-Gyamfi Sylvester 
Published: October 2022

Abstract

Although evidence abounds about low condom use among fisherfolks, leading to high human immunodeficiency virus (HIV) prevalence in fishing communities, there is dearth of information about the beliefs, attitudes, and behaviors of fishers regarding the use of condom. This article is a follow-up to a cross-sectional study that used a mix-method design for data collection and analysis to examine the connection between mobility and the risk of contracting HIV among 385 fishers in the Elmina fishing community of Ghana. The Health Belief Model is utilized in the discussions to analyze attitudes and actions toward condom use among the fishers in Elmina. There is a general low perception of HIV susceptibility and severity resulting in the low condom usage among the fisherfolks according to the results. The paper urges civil society organizations in the study area to investigate ways to incorporate the provision of free condoms to fishers and at the numerous fishing destination sites along the coast of Elmina. The regular use of condoms in Elmina and the nearby fishing areas should also be emphasized through this outreach or mobile HIV service outlets.
HPLC and HPLC-MS Analysis of Intestinal Elimination and Phase 2 Metabolism of 4'-hydroxy-4-Methoxychalcone and its Bis-Mannich Analog In The Rat
Authors: Bernardes Aline , Kuzma Mónika , Almási Attila , Mayer Mátyás , Pérez Caridad Noda , Perjési Pál 
Published: October 2022

Aims:
The aim was to study the elimination and Phase 2 biotransformation of 4'-hydroxy-4-methoxychalcone (1) and its bis-Mannich analog (2) in the small intestine of the rat.

Background:
Earlier studies indicated that chalcones are promising starting points for drug design. Aminomethylation of drugs is considered to improve their delivery into the human body.

Objectives:
To set up validated HPLC-UV methods to quantitate the investigated chalcones in the rat intestinal perfusates. Comparison of intestinal disappearance and Phase 2 metabolic profile of the 4’-hydroxychalcone (1) and a bis-Mannich analog (2).

Methods:
Chalcones 1 and 2 were luminally perfused in the small intestine of rats at a concentration of 240 μM and 280 μM, respectively. Analysis of the collected intestinal perfusate samples was performed by a validated HPLC-UV method. Using HPLC-MS, the samples were analyzed for Phase 2 metabolites as well.

Results:
Elimination kinetics of the two 4’-hydroxychalcones displayed characteristic differences having the nonpolar chalcone 1 higher elimination rate over the 90-minute ex vivo experiments. HPLC-MS analysis of the perfusates indicated the presence of glucuronide, sulfate, and glutathione conjugates in the parent molecules. Intestinal disappearance and sulfation of the bis-Mannich derivative 2 showed characteristic differences compared to 1

Conclusion:
The results demonstrate, to the best of our knowledge, for the first time, how the title structural modification of phenolic chalcones affects intestinal elimination and Phase 2 metabolism of the compounds

Highlights:
Study on ex vivo intestinal elimination of a 4'-hydroxy-4-methoxychalcone and its bis-Mannich analog.
Development of validated HPLC-UV methods for quantitation of 4’-hydroxychalcone derivatives in rat intestinal perfusates.
HPLC-MS identification of Phase 2 metabolites of 4’-hydroxychalcones in rat intestinal perfusates.