bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hyperexcitable superior colliculus and fatal brainstem spreading depolarization in a model of Sudden Unexpected Death in Epilepsy
Authors: Cain Stuart M. , Bernier Louis-Philippe , Zhang Yiming , Yung Andrew C. , Kass Jennifer , Bohnet Barry , Yang Yi , Gopaul Rayshad , Kozlowski Piotr , MacVicar Brian A. , Snutch Terrance P. 
Published: January 2022

Abstract
Cardiorespiratory arrest and death in mouse models of sudden unexpected death in epilepsy occur when spreading depolarization is triggered by cortical seizures and then propagates to the brainstem. However, the critical brain regions and the specific changes required to allow spreading depolarization to propagate to the brainstem under the relatively rare circumstances leading to a fatal seizure are unknown. We previously found that following cortical seizure-inducing electrical stimulation, spreading depolarization could occur in both the superior and inferior colliculi in Cacna1aS218L mice, but was never observed in wild-type animals or following non-seizure-inducing stimuli in Cacna1aS218L mice. Here, we show that optogenetic stimulation of the superior/inferior colliculi in Cacna1aS218L mice induces severe seizures, and resulting spreading depolarization in the superior/inferior colliculi that propagates to the brainstem and correlates with the respiratory arrest followed by cardiac arrest. Further, we show that neurons of the superior colliculus in Cacna1aS218L mice exhibit hyperexcitable properties that we propose underlie a distinct susceptibility to spreading depolarization. Our data suggest that the susceptibility of the superior colliculus to elicit fatal spreading depolarization is a result of either genetic or seizure-related alterations within the superior colliculus that may involve changes to structure, connectivity and/or excitability.
Replicable patterns of causal information flow between hippocampus and prefrontal cortex during spatial navigation and spatial–verbal memory formation
Authors: Das Anup , Menon Vinod 
Published: January 2022

AbstractInteractions between the hippocampus and prefrontal cortex (PFC) play an essential role in both human spatial navigation and episodic memory, but the underlying causal flow of information between these regions across task domains is poorly understood. Here we use intracranial EEG recordings and spectrally resolved phase transfer entropy to investigate information flow during two different virtual spatial navigation and memory encoding/recall tasks and examine replicability of information flow patterns across spatial and verbal memory domains. Information theoretic analysis revealed a higher causal information flow from hippocampus to lateral PFC than in the reverse direction. Crucially, an asymmetric pattern of information flow was observed during memory encoding and recall periods of both spatial navigation tasks. Further analyses revealed frequency specificity of interactions characterized by greater bottom-up information flow from hippocampus to PFC in delta–theta band (0.5–8 Hz); in contrast, top-down information flow from PFC to hippocampus was stronger in beta band (12–30 Hz). Bayesian analysis revealed a high degree of replicability between the two spatial navigation tasks (Bayes factor &gt; 5.46e+3) and across tasks spanning the spatial and verbal memory domains (Bayes factor &gt; 7.32e+8). Our findings identify a domain-independent and replicable frequency-dependent feedback loop engaged during memory formation in the human brain.
Implementation and Short-term Adverse Events of Anti-SARS-CoV-2 Vaccines in Inflammatory Bowel Disease Patients: An International Web-based Survey
Authors: Ellul Pierre , Revés Joana , Abreu Bárbara , Chaparro María , Gisbert Javier P , Allocca Mariangela , Fiorino Gionata , Barberio Brigida , Zingone Fabiana , Pisani Anthea , Cassar David , Michalopoulos George , Mantzaris Gerassimos , Koutroubakis Ioannis , Karmiris Konstantinos , Katsanos Konstantinos , Ďuricova Dana , Burisch Johan , Madsen Gorm Roager , Maaser Christian , Naila Arebi , Orfanoudaki Eleni , Milivojevic Vladimir , Buisson Anthony , Avedano Luisa , Leone Salvo , Torres Joana 
Published: January 2022

Abstract

Introduction
Anti-SARS-CoV-2 vaccine clinical trials did not include patients with immune-mediated conditions such as inflammatory bowel disease [IBD]. We aimed to describe the implementation of anti-SARS-CoV-2 vaccination among IBD patients, patients’ concerns, and the side effect profile of the anti-SARS-CoV-2 vaccines, using real-world data.

Methods
An anonymous web-based self-completed survey was distributed in 36 European countries between June and July 2021. The results of the patient characteristics, concerns, vaccination status, and side effect profile were analysed.

Results
In all 3272 IBD patients completed the survey, 79.6% had received at least one dose of anti-SARS-CoV-2 vaccine, and 71.7% had completed the vaccination process. Patients over 60 years old had a significantly higher rate of vaccination [p &lt; 0.001]. Patients’ main concerns before vaccination were the possibility of having worse vaccine-related adverse events due to their IBD [24.6%], an IBD flare after vaccination [21.1%], and reduced vaccine efficacy due to IBD or associated immunosuppression [17.6%]. After the first dose of the vaccine, 72.4% had local symptoms and 51.4% had systemic symptoms [five patients had non-specified thrombosis]. Adverse events were less frequent after the second dose of the vaccine and in older patients. Only a minority of the patients were hospitalised [0.3%], needed a consultation [3.6%], or had to change IBD therapy [13.4%] after anti-SARS-CoV-2 vaccination.

Conclusions
Although IBD patients raised concerns about the safety and efficacy of anti-SARS-CoV-2 vaccines, the implementation of vaccination in those responding to our survey was high and the adverse events were comparable to the general population, with minimal impact on their IBD.
Acute ischaemic stroke and its challenges for the intensivist
Authors: Robba Chiara , van Dijk Ewoud J , van der Jagt Mathieu 
Published: January 2022

Abstract
Acute ischaemic stroke (AIS) is responsible for almost 90% of all strokes and is one of the leading causes of death and disability. Acute ischaemic stroke is caused by a critical alteration in focal cerebral blood flow (ischaemia) from a variety of causes, resulting in infarction. The primary cerebral injury due to AIS occurs in the first hours, therefore early reperfusion importantly impacts on patient outcome (‘Time is brain’ concept). Secondary cerebral damage progressively evolves over the following hours and days due to cerebral oedema, haemorrhagic transformation, and cerebral inflammation. Systemic complications, such as pneumonia, sepsis, and deep venous thrombosis, could also affect outcome. The risk of a recurrent ischaemic stroke is in particular high in the first days, which necessitate particular attention. The role of intensive care unit physicians is therefore to avoid or reduce the risk of secondary damage, especially in the areas where the brain is functionally impaired and ‘at risk’ of further injury. Therapeutic strategies therefore consist of restoration of blood flow and a bundle of medical, endovascular, and surgical strategies, which—when applied in a timely and consistent manner—can prevent secondary deterioration due to cerebral and systemic complications and recurrent stroke and improve short- and long-term outcomes. A multidisciplinary collaboration between neurosurgeons, interventional radiologists, neurologists, and intensivists is necessary to elaborate the best strategy for the treatment of these patients.
Design of novel hybrid secondary metabolite targets to diguanylate cyclase ofAcinetobacter baumannii
Authors: Tiwari Monalisa , Joshi Richa , Tiwari Vishvanath 
Published: January 2022

AbstractBiofilm formation in bacteria is a resistance determinant and is positively regulated by cyclic diguanylate signaling. This signaling is a near universal signaling, and c-di-GMP produced by diguanylate cyclase (DGC) in this signaling is involved in different bacterial behaviors. The present study aims to find a plant-based novel hybrid therapeutic agent that can target the DGC of Acinetobacter baumannii. In this study, we have tried to design a hybrid molecule from the anti-biofilm plant secondary metabolites and screened its binding with the DGC of A. baumannii. The modeled and validated DGC was used to identify the active site and docking grid. Designed hybrid compounds were analysed for their interaction with the active site residues of DGC of A. baumannii. Further, the binding free energies of the docked complexes obtained from the Generalized Born model and Solvent Accessibility (MMGBSA) were analysed. The results indicated that VR-QEg-180 has a predicted high binding affinity with enzyme DGC as compared to other hybrids, parent secondary metabolites and positive control. Molecular dynamics simulation (MDS) analysis confirmed the interaction of VR-QEg-180 with DGC of the A. baumannii. The designed lead has favorable ADMET properties, has no human off-targets and has no predicted cytotoxicity in cell lines. Therefore, the designed hybrid molecule (VR-QEg-180) targeting the DGC of A. baumannii may play a very significant role in controlling this pathogen.
Unraveling the potential of non-conventional yeasts in biotechnology
Authors: Geijer C , Ledesma-Amaro R , Tomás-Pejó E 
Published: January 2022

ABSTRACT
Cost-effective microbial conversion processes of renewable feedstock into biofuels and biochemicals are of utmost importance for the establishment of a robust bioeconomy. Conventional baker's yeast Saccharomyces cerevisiae, widely employed in biotechnology for decades, lacks many of the desired traits for such bioprocesses like utilization of complex carbon sources or low tolerance towards challenging conditions. Many non-conventional yeasts (NCY) present these capabilities, and they are therefore forecasted to play key roles in future biotechnological production processes. For successful implementation of NCY in biotechnology, several challenges including generation of alternative carbon sources, development of tailored NCY and optimization of the fermentation conditions are crucial for maximizing bioproduct yields and titers. Addressing these challenges requires a multidisciplinary approach that is facilitated through the ‘YEAST4BIO’ COST action. YEAST4BIO fosters integrative investigations aimed at filling knowledge gaps and excelling research and innovation, which can improve biotechnological conversion processes from renewable resources to mitigate climate change and boost transition towards a circular bioeconomy. In this perspective, the main challenges and research efforts within YEAST4BIO are discussed, highlighting the importance of collaboration and knowledge exchange for progression in this research field.
Anatomy of the perirectal fascia at the level of rectosacral fascia revisited
Authors: Ghareeb Waleed M , Wang Xiaojie , Chi Pan , Zheng Zhifang , Zhao Xiaozhen 
Published: January 2022

AbstractBackgroundThe relative anatomical understanding of the perirectal fasciae is of paramount importance for the proper performance of total mesorectal excision (TME). This study was to demonstrate the planes of TME and validates the intraoperative findings using cadaveric observations.MethodsIn this combined retrospective and prospective study, bilateral attachment of the rectosacral fascia (RSF) was observed in 28 cadaveric specimens (male, n = 14; female, n = 14). From January 2018 to December 2019, surgical videos of 67 patients who underwent laparoscopic TME at the Affiliated Union Hospital of Fujian Medical University (Fuzhou, China) were reviewed and interpreted with the cadaveric findings.ResultsThe RSF (synonym: Waldeyer's fascia) is the end of the pre-hypogastric fascia at the level of S4 and comprises two layers (upper and lower). These two layers provide double fascial protection for the venous sacral plexus. It inserts into the fascia propria of the rectum along a broad horizontal arc that merges anterolaterally in an oblique downward direction until it meets the posterolateral merge of Denonvilliers' fascia at the lateral rectal ligament (LRL). This ligament does not look like a true ligament but is more likely to be a fascial combination that cushions the rectal innervation and middle rectal vessels.ConclusionsUnderstanding the lateral attachment of RSF and its contribution to LRL provides invaluable surgical guidance to dissect this critical area. Therefore, lateral dissection is proposed from the anterior to the posterior direction to find the correct plane that guarantees an intact mesorectal envelope to protect the important nearby nerve structures.
Fractional Leibniz Rules Associated to Bilinear Hermite Pseudo-Multipliers
Authors: Ly Fu Ken , Naibo Virginia 
Published: January 2022

Abstract
We obtain a fractional Leibniz rule associated to bilinear Hermite pseudo-multipliers in the context of Hermite Besov and Hermite Triebel–Lizorkin spaces. As a byproduct, we show that the classes of bounded functions in these spaces (which include Hermite Sobolev and Hermite Hardy–Sobolev spaces) are algebras under pointwise multiplication. To obtain these results we develop appropriate decompositions for bilinear pseudo-multipliers and molecular estimates for certain families of functions in the Hermite setting.
One Swab Fits All: Performance of a Rapid, Antigen-Based SARS-CoV-2 Test Using a Nasal Swab, Nasopharyngeal Swab for Nasal Collection, and RT–PCR Confirmation from Residual Extraction Buffer
Authors: Stokes William , Berenger Byron M , Scott Brittney , Szelewicki Jonas , Singh Takshveer , Portnoy Danielle , Turnbull LeeAnn , Pabbaraju Kanti , Shokoples Sandy , Wong Anita A , Gill Kara , Hu Jia , Tipples Graham 
Published: January 2022

Abstract

Background
Point-of-care SARS-CoV-2 antigen tests have great potential to help combat the COVID-19 pandemic. In the performance of a rapid, antigen-based SARS-CoV-2 test (RAT), our study had 3 main objectives: to determine the accuracy of nasal swabs, the accuracy of using nasopharyngeal swabs for nasal collection (nasalNP), and the effectiveness of using residual extraction buffer for real-time reverse-transcriptase PCR (RT–PCR) confirmation of positive RAT (rPan).

Methods
Symptomatic adults recently diagnosed with COVID-19 in the community were recruited into the study. Nasal samples were collected using either a nasalNP or nasal swab and tested immediately with the RAT in the individual’s home by a health care provider. 500 µL of universal transport media was added to the residual extraction buffer after testing and sent to the laboratory for SARS-CoV-2 testing using RT–PCR. Parallel throat swabs tested with RT–PCR were used as the reference comparators.

Results
One hundred and fifty-five individuals were included in the study (99 nasal swabs, 56 nasalNP). Sensitivities of nasal samples tested on the RAT using either nasal or nasalNP were 89.0% [95% confidence interval (CI) 80.7%–94.6%] and 90.2% (95% CI 78.6%–96.7%), respectively. rPan positivity agreement compared to throat RT–PCR was 96.2%.

Conclusions
RAT reliably detect SARS-CoV-2 from symptomatic adults in the community presenting within 7 days of symptom onset using nasal swabs or nasalNP. High agreement with rPan can avoid the need for collecting a second swab for RT–PCR confirmation or testing of variants of concern from positive RAT in this population.
Rodolphe (1845–1905) and Maurice Kann (1839–1906)
Authors: Prevost-Marcilhacy Pauline 
Published: January 2022

Abstract
This study of two important German–Jewish collectors who settled in Paris, after 1866, Maurice (1839–1906) and Rodolphe (1845–1905) Kann, examines their place in French society. Based on unpublished archives, in particular the discovery of their wills (Archives Nationales, Paris) and their correspondence with Wilhelm von Bode (Berlin), it helps us to understand the evolution of the brothers’ collections and their practice of donation. Paradoxically, these two collectors escape any classification. Are they collectors? Are they patrons? They appear on the fringes of the group of collector figures of the late 1800s, and their activities show that the frontier between personal pleasure and speculation is porous. The article also raises the question of the role of dealers in the construction of a business focused on collection at the fin-de-siècle.
Immune-related adverse events are clinical biomarkers to predict favorable outcomes in advanced renal cell carcinoma treated with nivolumab plus ipilimumab
Authors: Ueda Kosuke , Suekane Shigetaka , Kurose Hirofumi , Ito Naoki , Ogasawara Naoyuki , Hiroshige Tasuku , Chikui Katsuaki , Ejima Kazuhisa , Uemura Keiichiro , Nakiri Makoto , Nishihara Kiyoaki , Igawa Tsukasa 
Published: January 2022

Abstract

Background
Immune checkpoint inhibitors cause various immune-related adverse events. The present study examined the association between the incidence of immune-related adverse events and survival outcomes in patients treated with nivolumab plus ipilimumab for patients with advanced renal cell carcinoma. In addition, we compared the effect of adverse event profiles on survival for patients receiving nivolumab plus ipilimumab.

Methods
A total of 35 patients with advanced renal cell carcinoma who were treated with nivolumab plus ipilimumab from August 2018 to August 2021 were retrospectively reviewed and analyzed. Cox proportional hazards models were used for univariate and multivariate analyses, and hazard ratio and 95% confidence intervals were calculated.

Results
Of the 35 patients, 22 (62.9%) experienced immune-related adverse events. The median progression-free survival (P = 0.0012) and overall survival (P = 0.0147) were significantly longer in patients with immune-related adverse events than in those without immune-related adverse events. Multivariate analysis showed that the incidence of immune-related adverse events was an independent factor for progression-free survival (hazard ratio = 4.940, 95% confidence interval: 1.558–15.664, P = 0.0067). Skin reaction was a positive predictive immune-related adverse events for progression-free survival (hazard ratio = 9.322, 95% confidence interval: 1.954–44.475, P = 0.0051).

Conclusion
Patients with advanced renal cell carcinoma with immune-related adverse events had superior clinical outcomes of nivolumab plus ipilimumab treatment than those without immune-related adverse events. Skin immune-related adverse events may be effective biomarkers in patients with advanced renal cell carcinoma treated with nivolumab plus ipilimumab.
Development of tilt-scan system for differential phase contrast scanning transmission electron microscopy
Authors: Kohno Yuji , Nakamura Akiho , Morishita Shigeyuki , Shibata Naoya 
Published: January 2022

Abstract
Differential phase contrast (DPC) scanning transmission electron microscopy can directly visualize electromagnetic fields inside a specimen. However, their image contrast is not only sensitive to the electromagnetic fields in the sample, but also the changes in diffraction conditions such as sample bends or thickness changes. These additional contrasts are called diffraction contrasts, and sometimes make it difficult to extract pure electromagnetic field information from the experimental DPC images. In this study, we developed a beam scan system that can acquire many DPC images from the same sample region with arbitrarily varying incident beam tilt angles to the sample. Then, these images are precisely averaged to form tilt-scan averaged DPC images. It is shown that the diffraction contrast can be effectively reduced in the tilt-scan averaged DPC images.
Enhancing Conflict Resolution ‘ASEAN Way’: The Dispute Settlement System of the Regional Comprehensive Economic Partnership
Authors: Lando Massimo 
Published: January 2022

Abstract
This article examines the dispute settlement system of the Regional Comprehensive Economic Partnership (RCEP), adopted under the auspices of ASEAN on 15 November 2020. RCEP’s Chapter 19 on dispute settlement confirms and enhances the so-called ‘ASEAN way’, which promotes flexibility, consensus and the avoidance of direct confrontation in resolving inter-State controversies. This article discusses how RCEP enhances the ‘ASEAN way’ by reference to certain significant provisions of Chapter 19, also by comparison to the dispute settlement provisions of the WTO’s Dispute Settlement Understanding and other ASEAN+1 Free Trade Agreements.
Golgi anti-apoptotic proteins redundantly counteract cell death by inhibiting production of reactive oxygen species under endoplasmic reticulum stress
Authors: Tang Xiao-Han , Li Xin , Zhou Yan , He Yu-Ting , Wang Zhi-Ying , Yang Xue , Wang Wei , Guo Kun , Zhang Wei , Sun Yue , Li Hong-Qing , Li Xiao-Fang 
Published: January 2022

Abstract
Maintaining proteostasis in the endoplasmic reticulum (ER) is critical for cell viability and plant survival under adverse conditions. The unfolded protein response (UPR) pathways interact with reactive oxygen species (ROS) to precisely trigger adaptive outputs or cell death under ER stress with varying degrees. However, little information is known about the relationship between UPR signalling and ROS regulation. Here, Arabidopsis GOLGI ANTI-APOPTOTIC PROTEIN1 (GAAP1)-GAAP4 were found to play redundant positive roles under ER stress. Genetic analysis showed that GAAP4 played a role in INOSITOL-REQUIRING ENZYME (IRE1)-dependent and -independent pathways. In addition, GAAPs played negative roles to activate the adaptive UPR under conditions of stress. Quantitative biochemical analysis showed that mutations in GAAP genes decreased the oxidised glutathione content and altered the pattern of ROS and glutathione in early ER stress. When plants were challenged with unmitigated ER stress, mutations in GAAP advanced ROS accumulation, which was associated with a decline in adaptive UPR. These data indicated that GAAPs resist cell death by regulating glutathione content to inhibit ROS accumulation and maintain UPR during ER stress. They provide a basis for further analysis of the regulation of cell fate decision under ER stress.
Modelling annual scintillation arc variations in PSR J1643−1224 using the Large European Array for Pulsars
Authors: Mall G , Main R A , Antoniadis J , Bassa C G , Burgay M , Chen S , Cognard I , Concu R , Corongiu A , Gaikwad M , Hu H , Janssen G H , Karuppusamy R , Kramer M , Lee K J , Liu K , McKee J W , Melis A , Mickaliger M B , Perrodin D , Pilia M , Possenti A , Reardon D J , Sanidas S A , Sprenger T , Stappers B W , Wang L , Wucknitz O , Zhu W W 
Published: January 2022

ABSTRACT
In this work, we study variations in the parabolic scintillation arcs of the binary millisecond pulsar PSR J1643−1224 over five years using the Large European Array for Pulsars (LEAP). The two-dimensional (2D) power spectrum of scintillation, called the secondary spectrum, often shows a parabolic distribution of power, where the arc curvature encodes the relative velocities and distances of the pulsar, ionized interstellar medium, and Earth. We observe a clear parabolic scintillation arc, which varies in curvature throughout the year. The distribution of power in the secondary spectra is inconsistent with a single scattering screen, which is fully 1D or entirely isotropic. We fit the observed arc curvature variations with two models: an isotropic scattering screen and a model with two independent 1D screens. We measure the distance to the scattering screen to be in the range 114–223 pc, depending on the model, consistent with the known distance of the foreground large-diameter H ii region Sh 2-27 (112 ± 17 pc), suggesting that it is the dominant source of scattering. We obtain only weak constraints on the pulsar’s orbital inclination and longitude of ascending node, since the scintillation pattern is not very sensitive to the pulsar’s motion and the screen is much closer to the Earth than the pulsar. More measurements of this kind – where scattering screens can be associated with foreground objects – will help to inform the origins and distribution of scattering screens within our galaxy.