bluuebunny/crossref_metadata_2025_split huggingface dataset:
Idiosyncratic fitness costs of ampicillin-resistant mutants derived from a long-term experiment with Escherichia coli
Authors: Jordan Jalin A. , Lenski Richard E. , Card Kyle J. 
Published: February 2022

AbstractAntibiotic resistance is a growing concern that has prompted a renewed focus on drug discovery, stewardship, and evolutionary studies of the patterns and processes that underlie this phenomenon. A resistant strain’s competitive fitness relative to its sensitive counterparts in the absence of drug can impact its spread and persistence in both clinical and community settings. In a prior study, we examined the fitness of tetracycline-resistant clones that evolved from five different Escherichia coli genotypes, which had diverged during a long-term evolution experiment. In this study, we build on that work to examine whether ampicillin-resistant mutants are also less fit in the absence of the drug than their sensitive parents, and whether the cost of resistance is constant or variable among independently derived lines. Like the tetracycline-resistant lines, the ampicillin-resistant mutants were often less fit than their sensitive parents, with significant variation in the fitness costs among the mutants. This variation was not associated with the level of resistance conferred by the mutations, nor did it vary across the different parental backgrounds. In our earlier study, some of the variation in fitness costs associated with tetracycline resistance was explained by the effects of different mutations affecting the same cellular pathway and even the same gene. In contrast, the variance among the ampicillin-resistant mutants was associated with different sets of target genes. About half of the resistant clones suffered large fitness deficits, and their mutations impacted major outer-membrane proteins or subunits of RNA polymerases. The other mutants experienced little or no fitness costs and with, one exception, they had mutations affecting other genes and functions. Our findings underscore the importance of comparative studies on the evolution of antibiotic resistance, and they highlight the nuanced processes that shape these phenotypes.
The AAA+ ATPase RavA and its binding partner ViaA modulate E. coli aminoglycoside sensitivity through interaction with the inner membrane
Authors: Felix Jan , Bumba Ladislav , Liesche Clarissa , Fraudeau Angelique , Rébeillé Fabrice , El Khoury Jessica Y. , Huard Karine , Gallet Benoit , Moriscot Christine , Kleman Jean-Philippe , Duhoo Yoan , Jessop Matthew , Kandiah Eaazhisai , Barras Frédéric , Jouhet Juliette , Gutsche Irina 
Published: February 2022

AbstractEnteric bacteria have to adapt to environmental stresses in the human gastrointestinal tract such as acid and nutrient stress, oxygen limitation and exposure to antibiotics. Membrane lipid composition has recently emerged as a key factor for stress adaptation. The E. coli ravA-viaA operon is essential for aminoglycoside bactericidal activity under anaerobiosis but its mechanism of action is unclear. Here we characterise the VWA domain-protein ViaA and its interaction with the AAA+ ATPase RavA, and find that both proteins localise at the inner cell membrane. We demonstrate that RavA and ViaA target specific phospholipids and subsequently identify their lipid-binding sites. We further show that mutations abolishing interaction with lipids restore induced changes in cell membrane morphology and lipid composition. Finally we reveal that these mutations render E. coli gentamicin-resistant under fumarate respiration conditions. Our work thus uncovers a ravA-viaA-based pathway which is mobilised in response to antibiotics under anaerobiosis and has a major impact on cell membrane regulation.
Non-essential tRNA and rRNA modifications impact the bacterial response to sub-MIC antibiotic stress
Authors: Babosan Anamaria , Fruchard Louna , Krin Evelyne , Carvalho André , Mazel Didier , Baharoglu Zeynep 
Published: February 2022

AbstractAntimicrobial resistance (AMR) develops as a major problem in infectious diseases treatment. While antibiotic resistance mechanisms are usually studied using lethal antibiotic doses, lower doses allowing bacterial growth are now considered as factors influencing the development and selection of resistance. Based on high throughput transposon insertion sequencing (TN-seq) in V. cholerae, we have undertaken the phenotypic characterization of 23 transfer RNA (tRNA) and ribosomal RNA (rRNA) modifications deletion mutants, for which growth is globally not affected in the absence of stress. We uncover a specific involvement of different RNA modification genes in the response to aminoglycosides (tobramycin (TOB), gentamicin (GEN)), fluoroquinolones (ciprofloxacin (CIP)), β-lactams (carbenicillin (CRB)), chloramphenicol (CM) and trimethoprim (TRM). Our results identify t/rRNA modification genes, not previously associated to any antibiotic resistance phenotype, as important factors affecting the bacterial response to sub-MIC antibiotics from different families. This suggests differential translation and codon decoding as critical factors involved in the bacterial response to stress.
Apremilast prevents blistering in human epidermis by stabilization of keratinocyte adhesion in pemphigus
Authors: Sigmund Anna M. , Winkler Markus , Engelmayer Sophia , Egu Desalegn T. , Kugelmann Daniela , Radeva Mariya Y. , Bayerbach Franziska C. , Kotschi Stefan , Yeruva Sunil , Schmidt Enno , Yazdi Amir S. , Ghoreschi Kamran , Vielmuth Franziska , Waschke Jens 
Published: February 2022

AbstractPemphigus vulgaris (PV) is a life-threatening blistering skin disease caused by autoantibodies (PV-IgG) destabilizing desmosomal adhesion. Current therapies focus on suppression of autoantibody formation and thus treatments directly stabilizing keratinocyte adhesion would fulfill an unmet medical need. We here demonstrate that apremilast, a phosphodiesterase 4 inhibitor used e.g. in psoriasis, prevents blistering in PV. Apremilast abrogated PV-IgG-induced loss of keratinocyte cohesion in ex-vivo epidermis and in vitro. This was paralleled by inhibition of keratin retraction and desmosome splitting but affected neither desmoglein (Dsg) depletion nor Dsg3 binding properties.Apremilast induced phosphorylation of plakoglobin at serine 665 – a mechanisms which is known to stabilize cardiomyocyte cohesion. Interestingly, keratinocytes phospho-deficient at this side showed altered organization of Dsg1, Dsg3 and keratin filaments and impaired adhesion, which was not rescued by apremilast. These data identified a new mechanism of desmosome regulation and propose that apremilast is protective in pemphigus by stabilizing keratinocyte cohesion.
Compressive forces stabilise microtubules in living cells
Authors: Li Yuhui , Kučera Ondřej , Cuvelier Damien , Rutkowski David M. , Deygas Mathieu , Rai Dipti , Pavlovič Tonja , Vicente Filipe Nunes , Piel Matthieu , Giannone Gregory , Vavylonis Dimitrios , Akhmanova Anna , Blanchoin Laurent , Théry Manuel 
Published: February 2022

AbstractCell mechano-sensation and adaptation are supported by the actin network. The microtubule network is not considered to be directly sensitive to mechanical forces acting on a cell. However, recent studies on isolated microtubulesin vitrohave shown that bending forces have an impact on their structure, composition and lifespan, suggesting that, in a cellular context, microtubules may react to mechanical forces. We tested this hypothesis in living cells by subjecting them to cycles of compressive forces and found that microtubules became distorted, less dynamic and more stable. This mechano-stabilisation depends on CLASP2, which relocates from the end to the deformed shaft of microtubules. These results demonstrate that microtubules in living cells have mechano-responsive properties that allow them to resist and even counteract the forces to which they are subjected.
A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution
Authors: Ijaz Faryal , Nakazato Ryota , Setou Mitsutoshi , Ikegami Koji 
Published: February 2022

AbstractThe introduction of small insertion/deletion (indel) mutations in the coding region of genes by the site-specific nucleases such as Cas9 allows researchers to obtain frameshift null mutants. Technically simple and costly reasonable genotyping methods are awaited to efficiently screen the frameshift null mutant candidates. Here, we developed a simple genotyping method called DST-PCR (Double-strand break Site-Targeted PCR) using “face-to-face” primers where the 3’ ends of forward and reverse primers face each other at the position between 3-bp and 4-bp upstream of the PAM sequence, which is generally the Cas9-mediated double-strand break site. Generated amplicons are directly subjected to TBE-High-Resolution PAGE, which contains a high concentration of bis-acrylamide, for mutant clones detection with 1-bp resolution. We demonstrate six examples of CRISPR/Cas9-engineered knockout cells, to screen indels to obtain potential KO cell clones utilizing our approach. This method allowed us to detect 1-bp to 2-bp insertion and 1-bp to 4-bp deletion in one or both alleles of mutant cell clones. In addition, this technique also allowed the identification of heterozygous and homozygous biallelic functional KO candidates. Thus, DST-PCR coupled with TBE-High-Resolution PAGE is a simple method for genotyping of the CRISPR/Cas9-engineered cell lines, which can be performed without special equipment and techniques.
Factors determining teleworking before and during COVID-19: some evidence from Spain and Andalusia
Authors: Caparrós Ruiz Antonio 
Published: February 2022

PurposeThis study aims to analyse the inequalities in access to teleworking in Spain. More specifically, the study examines what factors determine remote working in a pre-pandemic period and during the state of alarm (March 2020). Moreover, the study examines whether telecommuting is related to the likelihood of suffering emotional disorders during lockdowns.Design/methodology/approachThe methodological approach followed to analyse access to teleworking mainly consists of the estimation of ordered response models. Two datasets are used: the first is the Survey on Equipment and Use of Information and Communication Technologies (ICTs) in Households, conducted by the Spanish National Statistics Institute (INE) in the pre-pandemic period (2018). The second is the Social Survey 2020. Habits and Living Conditions of the Andalusian Population during the State of Alarm, conducted by the Institute of Statistics and Cartography of Andalusia (IECA) once the state of alarm was declared in Spain.FindingsThe results obtained indicate that ICT training is a key element in helping to explain the likelihood of working from home. However, some groups of workers might experience difficulties in their transition to teleworking. This could increase labour market segmentation and hinder the transition to the knowledge economy. Moreover, the findings detect that employees working both on the company premises and from home are more likely to suffer emotional disorders.Originality/valueThe paper sheds new empirical evidence on teleworking in Spain and Andalusia, including some novel methodological aspects to estimate the regressors’ effects on the probability of working from home.
The influence of cross-cultural stable and dynamic competencies on expatriate adaptation and outcomes: the case of Taiwan
Authors: Wu Wann-Yih , Bui Tuan Anh , Dao Tuan Cong 
Published: February 2022

PurposeThis study investigates the influence of cross-cultural stable and dynamic competencies on expatriate adaptation and outcomes.Design/methodology/approachThis study surveys 10 expatriates in Taiwan and conducts qualitative analysis based on the newly collected original data.FindingsThe study's results confirm that cross-cultural stable and dynamic competencies improve expatriates' cross-cultural adaptation, enhancing expatriate outcomes in cross-cultural environments.Originality/valueThis study contributes to cross-cultural management research by clarifying the characteristics and action mechanisms of cross-cultural stable competencies (including cultural empathy, emotional stability, social initiative, open-mindedness and flexibility) and cross-cultural dynamic competencies (including relational skills, general self-efficacy and non-ethnocentrism) in the international business context. The study's findings suggest future research directions in the empirical investigation of the antecedents, characteristics and results of cross-cultural competencies, cross-cultural adaptation and expatriate outcomes.
A novel hybrid particle swarm optimization rat search algorithm for parameter estimation of solar PV and fuel cell model
Authors: Singla Manish Kumar , Nijhawan Parag , Oberoi Amandeep Singh 
Published: February 2022

Purpose
The purpose of the proposed hybrid method aims to increase population efficiency, and a local search is used to further improve the value of the global best solution. An experimental observation suggests that the model’s statistical outcomes are more aligned with the real-time experimental findings.

Design/methodology/approach
A novel metaheuristic efficient hybrid algorithm, i.e. hybrid particle swarm optimization rat search algorithm, is introduced and applied for parameter extraction of hybrid energy system. This proposed hybrid method rules out the chances of local minima, hence enhancing the precision of the parametric estimation. The parameter extraction and error is calculated for the solar photovoltaic (PV)–fuel cell system using the proposed algorithm.

Findings
Nonparametric statistical tests are also conducted to indicate the findings of the outcome parameters using various metaheuristic algorithms. The proposed algorithm is better than the rest of the compared algorithms in the study.

Originality/value
The authors proposed a novel algorithm, and this proposed algorithm is implemented on hybrid solar PV and fuel cell-based system for parameter extraction. The nonparametric test results clearly suggest that the proposed algorithm is far more effective for parameter estimation of the test system.
Integration of risk management within the building information modeling (BIM) framework
Authors: Moshtaghian Farzaneh , Noorzai Esmatullah 
Published: February 2022

PurposeThis research has been conducted with a view to creating a framework to integrate risk management based on building information modeling (BIM) information.Design/methodology/approachIn this research, all the information related to the construction of a residential project including 3D, 4D and 5D BIM models and the execution and control phases information was collected, and the risk list was determined for each activity accordingly.FindingsThe present study has suggested a framework for risk management in order to optimize project changes.Originality/valueThe lack of integration between 3D, 4D and 5D modeling besides execution information is a fundamental problem in many projects. The gap between these two groups of information will lead to improper management and late decisions, eventually imposing unforeseen delays and cost overruns. Risk management by the means of adopting a new approach has been addressed in recent studies using new methods, such as BIM and its associated technologies, some of which were mentioned in the review of theoretical literature in this research.
Adoptions of prefabrication in residential sector in China: agent-based policy option exploration
Authors: Du Hua , Han Qi , Sun Jun , Wang Cynthia Changxin 
Published: February 2022

PurposeThis study aims to evaluate the effectiveness of different prefabricated construction (PC) policies using a case study in Wuhan, considering the local context.Design/methodology/approachThe effectiveness of PC policies is falling behind expectations. The main reason lies in an insufficient understanding of the policy impacts. An agent-based model was built by choosing the residential sector in a typical large city of Wuhan, China, as the study case. Different cost reduction scenarios were introduced for investigating the PC policy effectiveness. The proposed model and simulation approach can be used for other cities and generalized to the whole Chinese PC industry with the potential to include more local policies and corresponding data.FindingsSimulation results show that carbon emission reduction will be between 60,000 and 80,000 tons with policy incentives, nearly double that of the no policy intervention scenario. The target of 30% PC in all new buildings by 2026 in China is achievable with the subsidy policies of linear cost reduction, or cost reduction conforms to the learning curve.Practical implicationsSimulation results of three kinds of policy show that subsidy policy optimization is necessary regarding reducing the level of subsidy needed. The carbon credit policy is not essential since it has little influence on PC development. Implementing the project procurement restriction policy is not recommended if the scale of development of PC is more important than achieving the development target.Originality/valueThis study can help the government and developers make better policy and strategic decisions on PC development and boost the sustainability transition of the construction industry.
Rural access programs in Yemen: delay of international funded road projects
Authors: Alashwal Niyazi , Alashwal Ali 
Published: February 2022

PurposeRoad projects are essential for poverty reduction and the socio-economic development of Yemeni people living in rural areas. Rural access programs, which are mainly sponsored by foreign countries and international organisations, are often delayed. The purpose of this paper is to identify the delay factors of rural road projects with a focus on stakeholder-related factors.Design/methodology/approachThe data was collected using a questionnaire survey from 164 participants representing 7 groups, namely, owner, government, donors, consultants, designers, contractors and beneficiaries. The data was analysed using the partial least squares path modelling (PLS-SEM).FindingsThe results show that the identified factors contribute to 16% of the variance of project delay. Donor and government-related factors have a significant relationship with delay. Further analysis of data using the IPMA procedure in PLS-SEM revealed the top factors contributing to delay, which are project selection criteria imposed by donors, government bureaucracy, decision-making process, weak laws and guidelines that regulate the work of project implementation units, procurement procedures, delay of payment to contractor and delay of transactions.Originality/valueThe timely completion of rural road projects can have a positive impact on the current and future rural access programs aiming to reduce poverty and increase access to health and education services of over 70% of Yemeni people living in rural areas. Such programs will reduce the misfortune of displaced people due to the ongoing war in the country.
Understanding tourists' memorable local food experiences and their consequences: the moderating role of food destination, neophobia and previous tasting experience
Authors: Badu-Baiden Frank , Kim Seongseop (Sam) , Xiao Honggen , Kim Jungkeun 
Published: February 2022

Purpose
This study aims to test a new model by examining the influence of memorable local food consumption experiences (MLFCEs) on international diners’ affective states, well-being and attitudinal loyalty. Local food tasting destination, local food neophobia and previous local food tasting experience are used as moderating variables.

Design/methodology/approach
A quantitative data collection method was used to source data from 900 US tourists to European countries (excluding the UK) and Asian countries. The data were quantitatively analyzed to examine the multidimensionality of tourists’ MLFCEs as well as their influence on outcome variables.

Findings
The findings indicate that MLFCEs significantly explain diners’ attitude toward local food, subjective well-being, intention to recommend and attitudinal loyalty. Also, three variables partially moderate the associations between the proposed constructs.

Research limitations/implications
Theoretically, the study enhances our understanding of the dimensional nature of tourists’ MLFCEs and how they inform the affective and behavioral states of tourists. Practically, it provides insights for local food businesses and destination marketing organizations concerning the composition of tourists’ MLFCEs and promotion of a tourism destination.

Originality/value
This study has quantitatively unraveled the dimensionality of tourists’ MLFCEs. It also developed an integrated model to test the predictive effect of MLFCEs on outcome variables and has provided a deeper understanding of the relationships, thereby enriching the literature and aiding the development of relevant theories.
Motivation behind review provision in online travel communities: do hometowner contributions matter?
Authors: Liang Sai , Ye Qiang , Zhang Xiaoxia , Law Rob , Gong Caiyan 
Published: February 2022

Purpose
Online reviews have become increasingly important and numerous studies have noted the effect of social factors on the review provision of users. The purpose of this study is to investigate how hometowner contributions, which are defined as prior reviews posted by users from the same city, affect the quality of reviews by focal users.

Design/methodology/approach
Based on the theories of competitive altruism, pure altruism and reciprocity, as well as several social influence theories, a conceptual framework is constructed to explain user contribution behavior. In addition, empirical models are established based on 831,737 reviews of 919 hotels on Tripadvisor.

Findings
The quality of reviews by hometowners can significantly motivate subsequent users to contribute high-quality reviews. This positive effect is stronger than the effect of previous contributions by non-hometowners. The effect of hometowner contribution is amplified in users with limited review-posting experience and/or in those from countries with a considerable cultural distance from the target destination.

Research limitations/implications
This study provides the hospitality literature with new insights into the effect of social factors on the review provision of users in the context of online hotel review websites. The results also present numerous practical implications for online travel communities.

Originality/value
This study is an early attempt to analyze the effect of prior hometowner contributions on the subsequent contribution decisions of focal users. Thus, this study provides a satisfactory starting point for determining whether the review provision of focal users can be affected differently by prior contributions from their peers from different categories.