bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-specific impacts of CACNB4 overexpression on dendritic spine density: relevance to schizophrenia
Authors: Sweet Robert , Parker Emily , Kindja Nathan , DeGiosio Rebecca , Salisbury Ryan , Krivinko Josh , Cheetham Claire , MacDonald Matthew 
Published: September 2022

Abstract

The voltage-gated calcium channel (VGCC) subunit complex is comprised of the α1 subunit, the ion permeable channel, and three auxiliary subunits: β, α2δ and γ. β is the most extensively studied auxiliary subunit and is necessary for forward trafficking of the α1 subunit to the plasma membrane. VGCCs mediate voltage-dependent movement of calcium ions into neuronal cytoplasm, including at dendrites, where intracellular calcium spikes initiate signaling cascades that shape the structural plasticity of dendritic spines. Genetic studies strongly implicate calcium signaling dysfunction in the etiology of neurodevelopmental disorders including schizophrenia. Dendritic spine density is significantly decreased in schizophrenia in primary auditory cortex where it is driven by loss of small spines, and small spine loss associated with increased peptide levels of ALFDFLK found in the VGCC β subunit β4. Overexpessing CACNB4 selectively reduced small spine density in cortical neuron cultures. We set out to validate this observation in an intact mammalian system within the relevant neurodevelopmental context. We overexpressed CACNB4 in early development and assessed spine density and morphology in adult male and female mouse cortex. We characterized β1–4 protein levels and β4 protein-protein interactions. Overexpression selectively reduced small spine density in female mice. This effect was not dependent on estrous stage. Instead it corresponded to sex differences in the murine β4 interactome. The VGCC subunit β1b was significantly enriched in the β4 interactome of male relative to female mice, and thus may have served to mitigate VGCC overexpression-mediated spine loss in male mice.
Angle-Independent Colored Fabrics Coated with Washable Antifouling Amorphous Photonic Crystals
Authors: Fu Yin , Deng Lingbing , Li Xue , Tan Haiying , Shi Jiuxiao sun , Shi Qingwen , Zheng Guang , Wu Yuxin , Zhu Liangbo , Hossain Zaki 
Published: September 2022

Abstract

Photonic crystals (PCs) prepared from monodispersed nanoparticles into regular lattices has attract great attention in the past decades. PCs have showed great advantages in textiles comparing with the traditional dyes due to their bright color, not fade and environment friendly. However, textiles colored by PCs generally do not have antifouling properties and poor stability on the fabrics makes them not resistant to wash. In this work, monodispersed SiO2 and polydimethylsiloxane (PDMS) with low glass transition temperature is chosen to make up the PCs coatings. To increase the stability of the PCs on the fabrics SiO2 nanoparticles was also modified by PDMS. Then the washability and antifouling properties of the PCs coated fabric was systematically studied. To further verify the availability of this method, fabrics with different chemical compositions, weaving modes, surface densities, and original color were used as the substrate to construct PCs-coatings. The influence of the fabrics structure on the structural color, reflectance spectrum, and stability of the coatings were further studied. This work will play as a guide for constructing washable and antifouling PCs on different fabric surfaces.
Comparison of hydromorphone and sufentanil in postoperative patient-controlled intravenous analgesia: a retrospective analysis
Authors: Zhang Ying , Liu Minjun , Chen Gang 
Published: September 2022

Abstract
Background
To compare the analgesic effect, PCA use and adverse events between sufentanil and hydromorphone used in patient-controlled intravenous analgesia (PCIA).
Methods
From June 2020 to May 2021, 1594 eligible postoperative patients who received PCIA were collected in this study. According to the type of opioid, patients were divided into two groups: sufentanil group and hydromorphone group. We used the Numerical Rating Scale (NRS), the Functional Activity Scale (FAS) and the Level of Sedation (LOS) to evaluate the analgesic effects of the two groups of patients. We also collected the main data of PCA use, effective number of compressions and adverse reactions.
Results
At 24 hours (h) after sugery, the FAS score in the sufentanil group was higher than that in the hydromorphone group (P < 0.05). Compared with the sufentanil group, the PCA use, total number of compressions and effective number of consumptions were significantly decreased in the hydromorphone group during a 48h period (P < 0.05). There were no statistical differences in NRS score, LOS score and adverse events between two groups at 24h and 48h after surgery.
Conclusion
Compared with sufentanil, under the similar analgesic effect, hydromorphone provided lower PCA use and effective number of compressions in PCIA. Our findings may provide useful evidence for more future studies related to postoperative analgesia.
Occurrence and source analysis of heavy metals and dissolved organic matter in Nanyi Lake
Authors: Guolian Li , Xiao Li , Xiaoyan Jiang , Yu Zhang , Haibin Li , Jiamei Zhang , Gege Cai , Kun Luo , Fazhi Xie 
Published: September 2022

Abstract
Nanyi Lake is a tributary in the lower reaches of the Yangtze River and the largest freshwater lake in southern Anhui. Anthropogenic activities have significantly affected the lake with the rapid development of the surrounding economy in recent years. This study collected a total of 39×2 water samples of surface water and overlying water, detected the heavy metal content (As, Cr, Cu, Mn, Ni, Pb, Sb, Zn, Fe, Cd), and analyzed the spectral characteristics of the dissolved organic matter (DOM). The results demonstrated that the content of heavy metals As, Cr, Cu, Ni, Pb, Zn, and Cd in the surface water and overlying water of Nanyi Lake was lower than the class III limits of the environmental quality standard for surface water (GB3838–2002). However, the heavy metals Fe, Mn, and Sb in the overlying and surface water have sites that exceed the limit of class III. The contour map of spatial distribution demonstrated different degrees of heavy metals (except Zn) enrichment near the Langchuan River in the East Lake District. Parallel factor analysis showed that the main components of DOM in Nanyi Lake were tryptophan-like, fulvic acid-like, and tyrosine-like, and the dissolved organic matter was primarily derived from autogenous endogenous sources. The principal component and correlation analysis showed that the heavy metals in Nanyi Lake were primarily derived from the production wastewater discharged by the surrounding industrial and mining enterprises through the rivers input, followed by the non-point source runoff input of the surrounding agricultural production and lake aquaculture.
Assembly of four indigenous chicken genomes reveal loss-of-function mutations as a critical driving force in chicken domestication and evolution
Authors: Su Zhengchang 
Published: September 2022

Abstract
Although multiple chicken (Gallus gallus) genomes have been assembled, no high-quality indigenous chicken genome is so far available, hampering the understanding of chicken evolution and domestication. Here, we assembled genomes of four indigenous chickens with distinct traits. With an N50 of 90.5~90.9 Mb, we achieved thus far the highest quality of chicken genome assembly. We identified a total of 1,420 new protein-coding genes in the four genomes, which are missing in the earlier annotations. Most of these new genes also are encoded and a few of them are pseudogenized in the earlier assembled chicken genomes. We recover 51 and 36 of the 274 and 174 previously presumed “missing” genes in birds and chickens, respectively. In contrary to the earlier conclusion, our results indicate that chickens have a similar number of protein-coding genes as other tetrapods do. Moreover, we identified a large number (385~684) of non-processed pseudogenes in each of the four indigenous chickens as well as in the earlier assemblies. These pseudogenes affect the chickens’ environment sensation, immunity and cell growth, while their occurring patterns reflect the phylogenetic relationships of the chickens. Therefore, in contrary to the earlier conclusion, loss-of-function mutations play important roles in chicken domestication and evolution.
Potential Antibacterial Co-culture Streptomyces flavalbus from Indonesian mangroves induces the production of pyocyanin by Pseudomonas aeruginosa
Authors: Azhar Nuril , Yudiati Ervia 
Published: September 2022

Abstract
The potential Actinobacteria from the sediment and root Soneratia alba with co-culture as an antibacterial agent. 28 strains of Actinobacteria were found, composed of 17 strains from the sediment and 11 strains from the endophyte root of Sonneratia alba. Out of 580 fermentation, 540 fermenter co-cultures and 34 single fermenter cultures were assessed. Twenty-one pairs of co-culture were resulted positively against Staphylococcus aureus, and 16 pairs were positive against Pseudomonas aeruginosa. The highest clearance zone against S. aureus was obtained from 10 (Actino)-Aspergillus fumigatus (dead body) extract (21.57 ± 0.90 mm) while against P. aeruginosa was resulted from co-culture strains 3/37 extract (19.18 ± 0.80 mm). Strain 41 was determined to be Streptomyces flavalbus (98.07%) with the accession number MZ540332 when co-cultured with Pseudomonas aeruginosa to produce pyocyanin. Other Actinobacteria (6, 10, and 37) strains were identified as Streptomyces albogriseolus (99.79%), Streptomyces labedae (99%), and Streptomyces rochei (93.52%). It is concluded that co-culture is a valuable strategy to develop the new Actinobacteria product as a powerful antibacterial agent.
Electrospun mats based on poly(vinyl fluoride-co-hexafluoropropylene) and hybrid carbon nanofillers as high performance ultrafiltration membranes
Authors: Scaffaro Roberto , Gammino Michele , Maio Andrea 
Published: September 2022

Abstract

Graphene oxide (GO) and carbon nanotubes (CNTs) were integrated at different mutual ratios into poly(vinyl fluoride-co-hexafluoropropylene) (PVDF-co-HFP) and electrospun to construct mats that were assessed for treating water contaminated by methylene blue (MB) via vacuum-assisted ultrafiltration. The materials were fully characterized from a morphological, physicochemical and mechanical point of view. The results revealed that such materials are suitable for being used as membranes for continuous processes, such as ultrafiltration. In particular, adding 2 wt.% of GO and CNTs gave the best performance, showing extremely high flux (800 L*m− 2*h− 1), excellent rejection (99%) and flux recovery ratios (93.3%), along with antifouling properties (irreversible and reversible fouling below 6% and 25%, respectively), and reusability. These outstanding outcomes were ascribed to the peculiar microstructure achieved, which endowed polymeric membranes with high roughness, wettability, and mechanical robustness.
The Effect of Empower program on Cognition and Quality of life of Vulnerable Elderly in the community, Nigeria
Authors: Farombi Temitope H. , Akinyemi Olajoke , Adelaja Damilola , Elugbadebo Olufisayo , Khan Hafiz 
Published: September 2022

Abstract
Background
The process of ageing involves a combination of biological, psychological, and social factors. The rising proportion of older adults and increase in life expectancy have increased interest in maintaining and promoting cognitive health in later life. Participation in cognitively stimulating activities has been considered to maintain or strengthen cognitive skills, thereby reducing age-related cognitive decline. An important aspect of healthy ageing is the promotion of good mental health and well-being in later life.
Objective
The aim of the study was to examine the effect older adults’ empowerment on their cognition, mental and physical well-being in the community.
Methodology
: A sample of 20 purposively selected older adults in the community were empowered in their different vocation of choice. Data was collected via in-depth interview and observations. An interpretive-descriptive analysis was used to facilitate data analysis.
Results
The associated benefits of empowerment of older adults to improve their cognition emerged into five themes: quality of life; increased physical function; social interaction; socio-economic effect; and mental stability.
Conclusion
Empowerment-based programs should be provided for older people in the society for them to take advantage of the opportunities to feel safe, supported and optimistic about life and its possibilities.
Proton Beam Dosimetry Using Cerenkov Radiation- a Characterization of the Relationship Between Light Emission and Dose
Authors: Thompson Steven 
Published: September 2022

Abstract
Purpose To study the relationship between Cerenkov photon emission and absorbed dose in water, soft tissue, and cortical bone for a primary proton beam. A proportionality constant between the number of Cerenkov photons and the absorbed dose is produced, which will aid in future in vivo dosimetry work.
Materials and Methods An ab initio description of the relation between Cerenkov emission and absorbed dose is derived from the Frank-Tamm formula, the Bethe-Bloch equation, and the relation between energy fluence, mass collision stopping power, and dose. A proportionality constant is introduced to quantify the amount of Cerenkov radiation emission per Gray of absorbed dose. MCNP 6.2 is then used to obtain said proportionality constant for a spread-out Bragg peak used in proton therapy at the University of Florida Proton Therapy Institute. A correlation between proton energy and Cerenkov emission is also obtained for a water, soft tissue, and cortical bone phantom for proton energies of 10 – 150 MeV in 10 MeV increments. The central axis depth dose curve for a solid water phantom was also produced and compared to the central axis Cerenkov emission profile to ascertain the feasibility of using Cerenkov output as an estimator of the Bragg peak location.
Results The cortical bone was found to emit the most Cerenkov photons, followed by water and then soft tissue. The cortical bone produced the most Cerenkov photons due to its having the largest Sellmeier coefficients, and therefore largest refractive index. The differences between the water and soft tissue Cerenkov output is likely due to the larger electron density for water. The proportionality constant between Cerenkov photon emission and absorbed dose was found to be 6.99 x 10 8 photons/Gray, which comports well with other studies performed for photons. The Cerenkov output was also found to be a poor predictor of the Bragg peak location for a proton beam.
Conclusions: The proportionality constant, which relates the quantity of Cerenkov photon emission to absorbed dose, was found and determined to be of the same order of magnitude as that for a primary photon beam. The Cerenkov light output was found to be an inadequate predictor of the Bragg peak location, and therefore cannot be used for Bragg peak dosimetry, which is of preeminent importance in proton therapy.
Multi-centre discriminating concentration determination of broflanilide and potential for cross- resistance to other public health insecticides in Anopheles vector populations
Authors: Portwood Natalie M. , Mbewe Njelembo J. , Tungu Patrick K. , Shayo Magreth F. , Mlay George , Small Graham , Snetselaar Janneke , Kristan Mojca , Levy Prisca , Walker Thomas , Kirby Matthew J. , Kisinza William , Mosha Franklin W. , Rowland Mark , Messenger Louisa A. 
Published: September 2022

Abstract
Novel insecticides are urgently needed to control insecticide resistant populations of Anopheles malaria vectors. Broflanilide acts as a non-competitive antagonist of the gamma-aminobutyric acid (GABA) receptor and has shown prolonged effectiveness as an indoor residual spraying (IRS) product (VECTRON™ T500) in experimental hut trials against pyrethroid-resistant vector populations. This multi-centre study expanded upon initial discriminating concentration testing of broflanilide, using six Anopheles insectary colonies (An. gambiae Kisumu KCMUCo, An. gambiae Kisumu NIMR, An. arabiensis KGB, An. arabiensis SENN, An. coluzzii N’Gousso and An. stephensi SK), representing major malaria vector species, to facilitate prospective susceptibility monitoring of this new insecticide; and investigated the potential for cross-resistance to broflanilide via the A296S mutation associated with dieldrin resistance (rdl). Across all vector species tested, the discriminating concentration for broflanilide ranged between LC99x2=1.126 - 54.00 μg/ml or LC95x3=0.7437 - 17.82 μg/ml. Lower concentrations of broflanilide were required to induce complete mortality of An. arabiensis SENN (dieldrin-resistant), compared to its susceptible comparator, An. arabiensis KGB, and there was no association between the presence of the rdlmechanism of resistance and survival in broflanilide bioassays, demonstrating a lack of cross-resistance to broflanilide. Study findings provide a benchmark for broflanilide susceptibility monitoring as part of ongoing VECTRON™ T500 community trials in Tanzania and Benin.
Toll-like receptor 4 signaling in neurons mediates cerebral ischemia/reperfusion injury
Authors: Liu Liang , Xu Tian-Ce , Zhao Zi-Ai , Zhang Nan-Nan , Li Jing , Chen Huisheng 
Published: September 2022

AbstractIn microglia, Toll-like receptor 4 (TLR4) is well known to contribute to neuroinflammatory responses following brain ischemia. Meanwhile, TLR4 is also expressed in neurons and can mediate the conduction of calcium (Ca2+) influx, but the mechanistic link between neuronal TLR4 signaling and brain ischemic injury is still poorly understood. Here, primary neuronal cell culture from TLR4 knockout mice and conditional knockout mice of TLR4 in glutamatergic neurons (TLR4cKO) were used to establish ischemic models in vitro and in vivo, respectively. We found that deleting TLR4 reduced the neuronal death and intracellular Ca2+increase induced by oxygen and glucose deprivation (OGD) or lipopolysaccharide treatment. Infarct volume and functional deficits also were alleviated in TLR4cKOmice following cerebral ischemia/reperfusion (I/R). Furthermore, TLR4 and N-methyl-d-aspartate receptor subunit 2B (NMDAR2B) were colocalized in neurons. Deleting TLR4 in neurons rescued phosphorylates NMDAR2B up-regulation induced by ischemia via Src kinase in vitro and in vivo. Downstream signaling of NMDAR2B, the interaction of neuronal nitric oxide synthase (nNOS) with postsynaptic density protein-95 (PSD-95) also was disrupted in TLR4cKOmice following cerebral I/R. Taken together, we described a novel molecular neuronal pathway that TLR4 signal in neurons play a crucial role in the neuronal death, and provided a new target for neuroprotection after ischemic stroke.
Biochemical and structural basis for a flavin-monooxygenase catalyzing oxepinone formation and the complete biosynthesis of vibralactone
Authors: Feng Ke-Na , Zhang Yue , Yang Yanlong , Zhang Mingfang , Liu Jikai , Pan Lifeng , Zeng Ying 
Published: September 2022

Abstract
Oxepinone rings represent one of structurally unusual motifs of natural products and the biosynthesis of oxepinones is not fully understood. 1,5-seco-vibralactone (3) features an oxepinone motif and is a stable metabolite isolated from mycelial cultures of the mushroom Boreostereum vibrans. Prior studies confirmed the cyclization of 3 to form vibralactone (1) whose β-lactone-fused bicyclic core originates from 4-hydroxybenzoate (2), yet it is still unknown how 4-hydroxybenzoate is converted to 3 especially for the oxepinone ring construction in the biosynthesis of 1. In this study, using activity-guided fractionation together with proteomic analyses, we identify a NADPH/FAD-dependent monooxygenase VibO as the key enzyme performing a crucial ring-expansive oxygenation on the phenol ring to generate the oxepin-2-one structure. The crystal structure of VibO reveals that it forms a dimeric phenol hydroxylase-like architecture featured with a unique substrate-binding pocket adjacent to the bound FAD. Importantly, relevant biochemical and structural analyses support a Baeyer-Villiger oxidation mechanism adopted by VibO for catalyzing the oxepinone formation. Finally, the complete five-enzyme vibralactone pathway has been functionally reconstructed both in vitro and in Escherichia coli.
Prediction of Seasonal Snow Accumulation and Depletion by SARIMA Model using MODIS data
Authors: Kumar Mohit , Tiwari Reet Kamal , Kumar Kamal , Rautela Kuldeep Singh 
Published: September 2022

Abstract

Autoregressive Integrated Moving Average (ARIMA) and seasonal ARIMA (SARIMA) models are statistical techniques generally used in analyzing and forecasting seasonal, periodic cyclic, and non-stationary time series data. This paper presents the use of the Seasonal Autoregressive Integrated Moving Average (SARIMA) method for developing a forecasting model that computes seasonal snow accumulation and depletion in the snow dominant area of the Beas river catchment. A time-series data of 8- days average snow covers acquired by Terra and Aqua sensors of MODIS (Moderate Resolution Imaging Spectro-radiometer) optical satellite has been utilized (2003 – 2018). The Box – Jenkins methodology has been performed separately by splitting yearly data into two main seasons snow accumulation (Oct. – Feb.) and snow depletion (March – Sept.). Two SARIMA models, one for snow accumulation as (1,1,1) (0,1,3)19 and the second for snow depletion as (1,1,1) (1,1,2)27 were identified by visual inspection of ACF and PACF plots using data (2003 – 2015) and then accuracy assessment has been done using performance criterion like Akaike’s Information Criterion (AIC), MSE and RSS, etc. The performance of the resulting models was then validated using data (2016 - 2018) and the comparison of both the models showed a good agreement between the simulated and observed data with a coefficient of determination (R2) of 0.829 in snow accumulation and 0.893 in snow depletion. Finally, the study advised, that the identified models could be adequate to forecast the weekly snow accumulation and depletion at least for the next 3- years to predict hydraulic events such as flood forecasting, runoff estimation, and hydropower assessment.
Incorporating autism symptoms into a transdiagnostic model of anxiety and depression symptoms results in an improved model and distinct factor structure for autistic youth
Authors: Rosen Tamara E. , Rodriguez-Seijas Craig , Gadow Kenneth D. , Lerner Matthew D. 
Published: September 2022

Abstract

The present study examined the extent to which autism symptoms relate to anxiety, depression, and AD + in autistic youth. Anxiety and depression symptoms were measured using a DSM rating scale. A CFA of the DSM model of anxiety and depression symptoms showed inadequate fit. An EFA of anxiety, depression symptoms supported a model with three factors: Anxiety and Depression, Social Anxiety, and Autism. A subset of autism symptoms loaded onto the anxiety and depression symptom factors. Both the DSM and the EFA model had inadequate fit indices, though the EFA showed better fit over the DSM model. In addition, previous research converges with the obtained EFA factors. Taken together, while further research is needed to determine how to best model these symptoms, the present findings can be used to inform future factor analytic studies and subsequent conceptualizations of anxiety, depression, and ASD symptoms.