bluuebunny/crossref_metadata_2025_split huggingface dataset:
The first reptilian allergen and major allergen for fish‐allergic patients: Crocodile β‐parvalbumin
Authors: Ruethers Thimo , Nugraha Roni , Taki Aya C. , O'Malley Andrea , Karnaneedi Shaymaviswanathan , Zhang Stephanie , Kapingidza A. Brenda , Mehr Sam , Kamath Sandip D. , Chruszcz Maksymilian , Mackay Graham , Campbell Dianne E. , Lopata Andreas L. 
Published: May 2022

AbstractBackgroundClinical cross‐reactivity between bony fish, cartilaginous fish, frog, and chicken muscle has previously been demonstrated in fish‐allergic patients. In indicative studies, two reports of anaphylaxis following the consumption of crocodile meat and IgE‐cross‐binding were linked to the major fish allergen parvalbumin (PV). This study investigates IgE‐binding proteins in crocodile meat with a focus on PV and their clinical relevance.MethodsProteins were extracted from muscle tissue of crocodile, three bony fish, and two cartilaginous fish. A cohort of fish‐allergic pediatric patients (n = 77) underwent allergen skin prick testing (SPT) to three fish preparations (n = 77) and crocodile (n = 12). IgE‐binding proteins were identified and quantified by SDS‐PAGE, mass spectrometric analyses, and immunoblotting using commercial and in‐house antibodies, as well as individual and pooled patients’ serum. PV isoforms were purified or recombinantly expressed before immunological analyses, including human mast cell degranulation assay.ResultsOf the tissues analyzed, PV was most abundant in heated crocodile preparation, triggering an SPT of ≥3 mm in 8 of 12 (67%) fish‐allergic patients. Seventy percent (31 of 44) of fish PV‐sensitized patients demonstrated IgE‐binding to crocodile PV. Crocodile β‐PV was the major IgE‐binding protein but 20‐fold less abundant than α‐PV. Cellular reactivity was demonstrated for β‐PV and epitopes predicted, explaining frequent IgE‐cross‐binding of β‐PVs. Both PV isoforms are now registered as the first reptile allergens with the WHO/IUIS (β‐PV as Cro p 1 and α‐PV as Cro p 2).ConclusionFish‐allergic individuals may be at risk of an allergy to crocodile and should seek specialist advice before consuming crocodilian meat.
Difficulties in providing genetic consultations by public health nurses in Japan
Authors: Obayashi Chisato , Asahara Kiyomi , Umeda Maki 
Published: May 2022

AbstractObjectiveThe objectives of this study were to identify the topics that Japanese public health nurses (PHNs) find difficult during genetic consultations and to identify the relationships between these difficulties and PHNs’ genetic education.MethodsWe conducted a mail survey delivered to PHNs employed by local Japanese governmental agencies in the Tokyo metropolitan area, between July and October 2015. The self‐administered questionnaire queried the (1) experience with genetic consultation, (2) perceived difficulties in genetic consultation, (3) genetics education background, and (4) demographics. The association between the perceived difficulties in genetic consultations and the genetics education opportunities was examined using logistic regression.ResultsOf the 907 mailed surveys, 536 PHNs responded (59.1% response rate). Most of the respondents (89.7%) had previously conducted genetic consultations. The majority of respondents (72.7%) had partaken in consultations regarding a possible hereditary disorder in the client or their families, and of those, 76.6% perceived difficulties during the genetic consultation. Respondents who had previously studied decision‐making support for genetic testing were significantly less likely to perceive difficulties in genetic consultations on prenatal diagnosis (OR = 0.04, 95% CI [0.00–0.73]).ConclusionsOur results suggest that PHNs receive training in genetic consultation, which can be integrated into everyday practice.
Dual role of specific promoter tandem repeats integrating epigenetic silencing with heat response
Authors: Matsusaka Daniel , Botto Javier F. , Sanchez Diego H. 
Published: May 2022

AbstractSatellites are ubiquitous noncoding tandemly repeated sequences, yet knowledge about their biological relevance is still scarce. In plants, the few described cases point to roles in heterochromatin biology and gene regulation; however, a direct link to plant stress responses is yet to be uncovered. We present evidence that particular non‐centromere tandem repeats may display a central regulatory role in the intersection between epigenetic silencing and gene expression in dynamic environments. Within the projected promoter of Arabidopsis thaliana's imprinted SDC locus, a transcriptional gene silencing targeted tandem‐repeated area largely mediates epigenetic suppression and imprinting. Here, we show that this area, possibly acting as a cis‐element/enhancer, appears necessary and sufficient for SDC's heat transcriptional activity in vegetative tissues. Our results indicate that these particular noncoding tandem repeats may be genic and exhibit dual roles, not only as silencers at normal temperatures but also facilitating expression upon stress. An unusual adaptive form of abiotic transcriptional control unrelated to canonical heat signaling is implied, emphasizing a potential importance of genomic satellites for plant environmental epigenetics.
Physiological and molecular aspects of seed longevity: exploring intra‐species variation in eight Pisum sativum L. accessions
Authors: Gianella Maraeva , Doria Enrico , Dondi Daniele , Milanese Chiara , Gallotti Lucia , Börner Andreas , Zannino Lorena , Macovei Anca , Pagano Andrea , Guzzon Filippo , Biggiogera Marco , Balestrazzi Alma 
Published: May 2022

AbstractConservation of plant genetic diversity is fundamental for crop improvement, increasing agricultural production and sustainability, especially in the face of climatic changes. Although seed longevity is essential for the management of seed banks, few studies have, so far, addressed differences in this trait among the accessions of a single species. Eight Pisum sativum L. (pea) accessions were investigated to study the impact of long‐term (approximately 20 years) storage, aiming to reveal contrasting seed longevity and clarify the causes for these differences. The outstanding seed longevity observed in the G4 accession provided a unique experimental system. To characterize the biochemical and physical status of stored seeds, reactive oxygen species, lipid peroxidation, tocopherols, free proline and reducing sugars were measured. Thermoanalytical measurements (thermogravimetry and differential scanning calorimetry) and transmission electron microscopy combined with immunohistochemical analysis were performed. The long‐lived G4 seeds neither consumed tocopherols during storage nor showed free proline accumulation, as a deterioration hallmark, whereas reducing sugars were not affected. Thermal decomposition suggested a biomass composition compatible with the presence of low molecular weight molecules. Expansion of heterochromatic areas and reduced occurrence of γH2AX foci were highlighted in the nucleus of G4 seeds. The longevity of G4 seeds correlates with the occurrence of a reducing cellular environment and a nuclear ultrastructure favourable to genome stability. This work brings novelty to the study of within‐species variations in seed longevity, underlining the relevance of multidisciplinary approaches in seed longevity research.
Cellulose accumulation mediated by PbrCSLD5, a cellulose synthase‐like protein, results in cessation of pollen tube growth in Pyrus bretschneideri
Authors: Li Xian , Tang Chao , Li Xiaoqiang , Zhu Xiaoxuan , Cai Yiling , Wang Peng , Zhang Shaoling , Wu Juyou 
Published: May 2022

AbstractCellulose, a key component of the cell wall, plays an important role in maintaining the growth of pollen tubes. However, the molecular mechanism of cellulose participating in the cessation of pear pollen tube growth remains unclear. Here, we reported that at 15 h post‐cultured (HPC), the slow‐growth pear pollen tubes showed thickened cell walls and cellulose accumulation in the inner wall. Transcriptome data and quantitative real‐time PCR analysis showed that PbrCSLD5, a cellulose synthesis‐like gene, was highly expressed in the 15 HPC pear pollen tubes. Knockdown of PbrCSLD5 caused a decrease in cellulose content in pear pollen tubes. Moreover, PbrCSLD5 overexpression in Arabidopsis resulted in the accumulation of cellulose and disruption of normal pollen tube growth. Transcription factor PbrMADS52 was found to bind to the promoter of PbrCSLD5 and enhanced its expression. Our results suggested that the PbrMADS52–PbrCSLD5 signaling pathway led to increased cellulose content in the pear pollen tube cell wall, thereby inhibiting pollen tube growth. These results provided new insights into the regulation of pollen tube growth.
Transcriptome analysis and identification of genes associated with oil accumulation in upland cotton
Authors: Song Jikun , Pei Wenfeng , Wang Nuohan , Ma Jianjiang , Xin Yue , Yang Shuxian , Wang Wei , Chen Quanjia , Zhang Jinfa , Yu Jiwen , Wu Man , Qu Yanying 
Published: May 2022

AbstractCotton is not only the most important fiber crop but also the fifth most important oilseed crop in the world because of its oil‐rich seeds as a byproduct of fiber production. By comparative transcriptome analysis between two germplasms with diverse oil accumulation, we reveal pieces of the gene expression network involved in the process of oil synthesis in cottonseeds. Approximately, 197.16 Gb of raw data from 30 RNA sequencing samples with 3 biological replicates were generated. Comparison of the high‐oil and low‐oil transcriptomes enabled the identification of 7682 differentially expressed genes (DEGs). Based on gene expression profiles relevant to triacylglycerol (TAG) biosynthesis, we proposed that the Kennedy pathway (diacylglycerol acyltransferase‐catalyzed diacylglycerol to TAG) is the main pathway for oil production, rather than the phospholipid diacylglycerol acyltransferase‐mediated pathway. Using weighted gene co‐expression network analysis, 5312 DEGs were obtained and classified into 14 co‐expression modules, including the MEblack module containing 10 genes involved in lipid metabolism. Among the DEGs in the MEblack module,GhCYSD1was identified as a potential key player in oil biosynthesis. The overexpression ofGhCYSD1in yeast resulted in increased oil content and altered fatty acid composition. This study may not only shed more light on the underlying molecular mechanism of oil accumulation in cottonseed oil, but also provide a set of new gene for potential enhancement of oil content in cottonseeds.
Production of $$\Omega NN$$ and $$\Omega \Omega N$$ in ultra-relativistic heavy-ion collisions
Authors: Zhang Liang , Zhang Song , Ma Yu-Gang 
Published: May 2022

AbstractEven though lots of $$\Lambda $$
Λ
-hypernuclei have been found and measured, multi-strangeness hypernuclei consisting of $$\Omega $$
Ω
are not yet discovered. The studies of multi-strangeness hypernuclei help us further understand the interaction between hyperons and nucleons. Recently the $$\Omega N$$

Ω
N

and $$\Omega \Omega $$

Ω
Ω

interactions as well as binding energies were calculated by the HAL-QCD’s lattice Quantum Chromo-Dynamics (LQCD) simulations and production rates of $$\Omega $$
Ω
-dibaryon in Au + Au collisions at RHIC and Pb + Pb collisions at LHC energies were estimated by a coalescence model. The present work discusses the production of more exotic triple-baryons including $$\Omega $$
Ω
, namely $$\Omega NN$$

Ω
N
N

and $$\Omega \Omega N$$

Ω
Ω
N

as well as their decay channels. A variation method is used in calculations of bound states and binding energy of $$\Omega NN$$

Ω
N
N

and $$\Omega \Omega N$$

Ω
Ω
N

with the potentials from the HAL-QCD’s results. The productions of $$\Omega NN$$

Ω
N
N

and $$\Omega \Omega N$$

Ω
Ω
N

are predicted by using a blast-wave model plus coalescence model in ultra-relativistic heavy-ion collisions at $$\sqrt{s_{NN}} = 200$$

s

NN

=
200

GeV and 2.76 TeV. Furthermore, plots for baryon number dependent yields of different baryons (N and $$\Omega $$
Ω
), their dibaryons and hypernuclei are made and the production rate of a more exotic tetra-baryon ($$\Omega \Omega NN$$

Ω
Ω
N
N

) is extrapolated.
R &D of wavelength-shifting reflectors and characterization of the quantum efficiency of tetraphenyl butadiene and polyethylene naphthalate in liquid argon
Authors: Araujo G. R. , Baudis L. , McFadden N. , Krause P. , Schönert S. , Wu V. H. S. 
Published: May 2022

AbstractDetectors based on liquid argon (LAr) often require surfaces that can shift vacuum ultraviolet (VUV) light and reflect the visible shifted light. For the LAr instrumentation of the LEGEND-200 neutrinoless double beta decay experiment, several square meters of wavelength-shifting reflectors (WLSR) were prepared: the reflector Tetratex® (TTX) was in-situ evaporated with the wavelength shifter tetraphenyl butadiene (TPB). For even larger detectors, TPB evaporation will be more challenging and plastic films of polyethylene naphthalate (PEN) are considered as an option to ease scalability. In this work, we first characterized the absorption (and reflectivity) of PEN, TPB (and TTX) films in response to visible light. We then measured TPB and PEN coupled to TTX in a LAr setup equipped with a VUV sensitive photomultiplier tube. The effective VUV photon yield in the setup was first measured using an absorbing reference sample, and the VUV reflectivity of TTX quantified. The characterization and simulation of the setup along with the measurements and modelling of the optical parameters of TPB, PEN and TTX allowed to estimate the absolute quantum efficiency (QE) of TPB and PEN in LAr (at 87K) for the first time: these were found to be above 67 and 49%, respectively (at 90% CL). These results provide relevant input for the optical simulations of experiments that use TPB in LAr, such as LEGEND-200, and for experiments that plan to use TPB or PEN to shift VUV scintillation light.
Nonlinear dynamical Casimir effect at weak nonstationarity
Authors: Trunin Dmitrii A. 
Published: May 2022

AbstractWe show that even small nonlinearities significantly affect particle production in the dynamical Casimir effect at large evolution times. To that end, we derive the effective Hamiltonian and resum leading loop corrections to the particle flux in a massless scalar field theory with time-dependent Dirichlet boundary conditions and quartic self-interaction. To perform the resummation, we assume small deviations from the equilibrium and employ a kind of rotating wave approximation. Besides that, we consider a quantum circuit analog of the dynamical Casimir effect, which is also essentially nonlinear. In both cases, loop contributions to the number of created particles are comparable to the tree-level values.
Using mobile money data and call detail records to explore the risks of urban migration in Tanzania
Authors: Lavelle-Hill Rosa , Harvey John , Smith Gavin , Mazumder Anjali , Ellis Madeleine , Mwantimwa Kelefa , Goulding James 
Published: May 2022

AbstractUnderstanding what factors predict whether an urban migrant will end up in a deprived neighbourhood or not could help prevent the exploitation of vulnerable individuals. This study leveraged pseudonymized mobile money interactions combined with cell phone data to shed light on urban migration patterns and deprivation in Tanzania. Call detail records were used to identify individuals who migrated to Dar es Salaam, Tanzania’s largest city. A street survey of the city’s subwards was used to determine which individuals moved to more deprived areas.t-tests showed that people who settled in poorer neighbourhoods had less money coming into their mobile money account after they moved, but not before. A machine learning approach was then utilized to predict which migrants will move to poorer areas of the city, making them arguably more vulnerable to poverty, unemployment and exploitation. Features indicating the strength and location of people’s social connections in Dar es Salaam before they moved (‘pull factors’) were found to be most predictive, more so than traditional ‘push factors’ such as proxies for poverty in the migrant’s source region.
Fermion mass hierarchy and lepton flavor violation using CP symmetry
Authors: Ganguly Joy 
Published: May 2022

In this paper, we present a model which employs [Formula: see text] symmetries where quark mixing and charged leptons masses are explained by following some texture. To achieve neutrino mass and mixing, we write a nonrenormalizable Lagrangian using type II seesaw mechanism by assuming [Formula: see text] couplings predicting no CP violation. Then, we calculate the value of the couplings for both normal and inverted ordering of neutrino masses. We propose a mechanism of ultraviolet (UV) completion that motivates the higher-dimensional Lagrangian in the neutrino sector. We also calculate the branching fraction of lepton flavor violating decays driven by triplet scalar for the model. Later, it is shown that the model can also accommodate maximal CP violation by breaking the CP symmetry spontaneously.
Comparative Analysis of MultiCriteria Inventory Classification Models for ABC Analysis
Authors: Kaabi Hadhami 
Published: May 2022

ABC analysis is a commonly used inventory classification technique which consists in splitting a large number of inventory items into three categories, A, B and C: category A consists in the most important items, category B consists in the moderately important items and category C consists in the least important ones. Through this classification, inventory items are managed in an efficient way. In this paper, we argue the benefits of cross-fertilization of both Artificial Intelligence (AI) and MultiCriteria Decision Making (MCDM) techniques to carry out the ABC classification of inventory items. For this purpose, we develop some new hybrid inventory classification models based on metaheuristics (AI techniques) to generate the criteria weights and on Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) method (MCDM technique) to compute the overall weighted score of each item on which the ABC classification is performed. To evaluate the effectiveness of the proposed classification models with respect to some classification models from the literature, a comparative study — based on a service-cost analysis and three real datasets — is conducted. The computational analysis demonstrates that our proposed hybrid models are competitive and produce satisfactory results. The results have also shown, that our proposed models outperform some existing models from the literature.
O modelo de “letramentos acadêmicos”: teoria e aplicações
Authors: Komesu Fabiana Cristina , Fischer Adriana 
Published: May 2022

Embora o termo “letramentos acadêmicos” tenha sido originalmente desenvolvido visando ao estudo de letramentos em nível superior, o conceito também se aplica ao período da pré-escola ao ensino médio. Uma perspectiva dos letramentos acadêmicos concebe leitura e escrita como práticas sociais que variam segundo contexto, cultura e gênero (Barton; Hamilton, 1998; Street, 1984, 1985). As práticas de letramento de disciplinas acadêmicas podem ser entendidas como práticas sociais variadas associadas a diferentes comunidades. Além disso, uma perspectiva dos letramentos acadêmicos também leva em consideração letramentos não diretamente relacionados a temas e disciplinas, mas a discursos institucionais mais amplos e a gêneros. Do ponto de vista do estudante, um traço dominante das práticas de letramento acadêmico é a exigência de mudança de estilo de escrita e gênero segundo contexto. O objetivo é dispor de modo conjunto de práticas de letramento apropriadas a cada cenário e lidar com sentidos sociais e identidades que cada prática evoca.
Effect of WC particles on the mechanical behavior and machinability of aluminum matrix composites
Authors: Kara Memduh , Coskun Tolga , Gunoz Alper 
Published: May 2022

Aluminum-based metal matrix composites are extensively used in applications in many industrial fields, especially in the aviation and automotive industry. The usage rate is increasing day by day. Therefore, it is very important to improve the mechanical properties of the composite structure. For this purpose, in this study, 3, 5, and 7 wt.% tungsten carbide (WC) reinforced aluminum matrix composite material was produced by stir casting method. The mechanical properties (tensile strength, hardness, wear) of the produced composite materials and their effect on machinability were investigated. The machinability process was performed in constant cutting speed, constant cutting depth, three different feed rates (0.05, 0.1, and 0.2 mm·rev−1), and dry environmental conditions. Wear tests were performed on dry sliding conditions, under 25 N load, covering different distances (150, 300, 450, and 600 m). In addition, the microstructure of the composite samples was analyzed using optical microscopy and scanning electron microscope. The phases in the composite material were determined using the X-ray diffraction technique. As a result of the experiments, with the addition of WC particles, the mechanical properties of the materials such as tensile strength, hardness, and wear resistance have increased and the machinability capability has decreased.
Safe care for premature babies at home: Parenting and stimulating development
Authors: Pancieri Letícia , Silva Rosane M M , Wernet Monika , Fonseca Luciana M M , Hameed Shaffa , Mello Debora F 
Published: May 2022

Parental daily care and adequate stimuli are extremely important for development and safety of premature babies at home. This study aimed to analyze safe home care for babies born under 32 weeks from parents’ perspectives, with a view to a longitudinal promotion of baby development. A qualitative study, based on philosophical hermeneutic approach proposed by Hans-Georg Gadamer, in which dialogue as a principle provides understanding and fusion of experiences and knowledge. Semi-structured interviews were conducted with 18 parents of premature babies under 1 year of age. Thematic analysis proposed by Braun and Clarke was applied using an inductive approach. Elements related to safe care were identified: home arrival, safe home care: preserving baby health and development, support for safe home care, and development of parental care to promote baby safety. These elements can provide a basis for safe home care that needs to be reinforced longitudinally to increase particularities of baby protection, avoid accidents and illnesses, and improve appropriate developmental stimuli and positive parenting.