bluuebunny/crossref_metadata_2025_split huggingface dataset:
NLRX1 knockdown attenuates pro-apoptotic signaling and cell death in pulmonary hyperoxic acute injury
Authors: Kim Hye Rin , Kim Mi Na , Kim Eun Gyul , Leem Ji Su , Baek Seung Min , Lee Yu Jin , Kim Kyung Won , Kang Min Jong , Song Tae Won , Sohn Myung Hyun 
Published: October 2022

Abstract
Hyperoxia is frequently used for treating acute respiratory failure, but it can cause acute lung injury. Nucleotide-binding domain and leucine-rich-repeat-containing family member X1 (NLRX1) is localized in mitochondria and related to reactive oxygen species production, inflammation, and apoptosis, which are the features of hyperoxic acute lung injury (HALI). However, the contribution of NLRX1 in HALI has not been addressed, so we designed to demonstrate the role of NLRX1 in hyperoxia. A murine model of HALI was generated in wild-type mice (WT) and NLRX1−/− mice by exposing them to over 95% oxygen for 72 h. As a result, NLRX1 expression was elevated in mice exposed to hyperoxia. In acute lung injury, levels of inflammatory cells, protein leakage, cell cytotoxicity, and pro-inflammatory cytokines were diminished in NLRX1−/− mice compared to WT mice. In survival test, NLRX1−/− mice showed alleviated mortality under hyperoxic conditions, and apoptotic cell death and caspase expression and activity were reduced in NLRX1−/− mice. Furthermore, levels of MAPK signaling proteins ERK 1/2, JNK, and p38 were decreased in NLRX1-deficient mice than in WT mice exposed to hyperoxia. This study reveals that the genetic deficiency of NLRX1 dampens hyperoxia-induced apoptosis, suggesting NLRX1 acts as a pivotal regulator of HALI.
Design and implementation of three-layer mesoporous silica coating for tri-wavelength broadband antireflection by block copolymer assisted sol-gel method
Authors: Qu Jingxuan , Jia Hongbao , Wang Wenwen , Wang Ying , Zhu Shihai 
Published: October 2022

Abstract
A three-layer tri-wavelength broadband antireflective (AR) coating has been successfully fabricated on quartz substrate via a sol-gel route using acid-catalyzed silica sols. An ethylene oxide-propylene oxide-ethylene oxide tribolock copolymer is used as template to prepare ordered mesoporous SiO2 films. Assisted by Filmstar thin film design software, film thickness for each layer is optimized on the basis of actual optical constants of the three mesoporous silica films. The three layers generate a reasonable refractive index gradient from air and thus the obtained AR coating possess high transmittance of 99.24%, 99.66% and 99.64% at 351 nm, 527 nm and 1053 nm, respectively. The mesoporous SiO2 films with tough skeleton despite different porosity endow the coating good abrasion-resistance, and 1H, 1H, 2H, 2H-Perfluorodecyltriethoxysilane is further used to modify the surface of the AR coating, which can improve the experimental stability of the coating. This work provides beneficial references for AR coating production of sol-gel technique.
Clinical Efficacy of General Anesthesia Versus Local Anesthesia for Percutaneous Transforaminal Endoscopic Discectomy: a retrospective cohort study
Authors: Wu Zhihua , He Jiahui , Cheng Huantong , Lin Shaohao , Zhang Peng , Liang De , Jiang Xiaobing , Cui Jianchao 
Published: October 2022

Abstract
Background
Local anesthesia(LA) is recommended for percutaneous transforaminal endoscopic discectomy(PTED), but satisfactory pain management is not mostly achieved.The goal of this study was to examine the clinical efficacy of PTED for lumbar disc herniation when performed under local anaesthetic versus general anesthesia (GA).
Methods
From August 2018 to August 2020, the clinical data of 108 patients treated with PTED were evaluated and separated into two groups: LA and GA. General information and clinical outcomes of patients were included. Visual analog scale (VAS) and Oswestry disability index (ODI) were recorded before operation, 1 week after operation, and 1 year after operation. In addition, VAS for back pain and leg pain on the second postoperative day were also recorded.
Results
We divided the patients into two groups: 72 in LA and 36 in GA. There were no significant differences in gender, age, course of disease, body mass index, surgical segment, duration of operation, intraoperative bleeding, time of fluoroscopy, length of hospital stay, total hospitalization cost reoperation, surgical satisfaction, Macnab satisfaction, complications, preoperative and 1 year postoperatively VAS for back pain and leg pain and ODI,VAS for leg pain on the second day and 1 week postoperatively between the two groups (P༞0.05). VAS for back pain in GA group on the second day postoperatively, as well as the VAS for back pain and ODI at one week postoperatively, were better than those in LA group. (P < 0.05). However, the total hospitalization cost in LA group was significantly lower than that in GA group (P < 0.05). Further analysis of different ages in the two groups showed that there were significant differences in the VAS for back pain on the second day postoperatively and ODI at 1 week postoperatively in the middle-aged group (45 ≤ Y ≤ 59), as well as the VAS for back pain on the second day postoperatively in the senior group (Y ≥ 60) (P < 0.05). However, there were no significant difference among other groups (P > 0.05).
Conclusion
Long-term outcomes were similar for both PTED under LA and GA, while GA group had better short-term outcomes, especially in middle-aged and elderly patients.
Experimental Semiotics: A Systematic Categorization of Experimental Studies on the Bootstrapping of Communication Systems
Authors: Delliponti Angelo , Raia Renato , Sanguedolce Giulia , Gutowski Adam , Pleyer Michael , Sibierska Marta , Placiński Marek , Żywiczyński Przemysław , Wacewicz Sławomir 
Published: October 2022

Abstract
Experimental Semiotics (ES) is the study of "novel forms of communication which people develop when they cannot use pre-established communication" (Galantucci 2012). Thus, it tackles pragmatics in a “pure”, radical sense, capturing the process of creating the relation between signs and their interpreters as biological, psychological, and social beings (see e.g. Morris 1938 or the notion of “intermediary pragmatics” in Bar-On 2021). Broadly defined ES has arguably become the most prolific experimental approach in language evolution research. In our paper we report the results of a study on the scope of recent ES research concerned with the emergence of novel communication systems and evaluate the ways in which it is relevant to the study of pragmatics from an evolutionary perspective. We have coded for multiple levels across 13 dimensions related to the properties of the emergent communication systems or properties of the study designs – such as coordination versus referential tasks, modality of communication, the absence or presence of turn-taking, sample size or director/matcher role distribution. We discuss our findings and our classification, focusing on the advantages and limitations of those tendencies in ES, and in particular their ecological validity in the context of bootstrapping communication and the evolution of language.
A Regressive Machine-Learning Approach to the Non-linear Complex FAST Model for Hybrid Floating Offshore Wind Turbines with Integrated Oscillating Water Columns
Authors: Ahmad Irfan , M’zoughi Fares , Aboutalebi Payam , Garrido Izaskun , Garrido Aitor J. 
Published: October 2022

Abstract
Offshore wind energy is getting increasing attention as a clean alternative to the currently scarce fossil fuels mainly used in Europe’s electricity supply. The further development and implementation of this kind of technology will help fighting global warming, allowing a more sustainable and decarbonized power generation. In this sense, the integration of Floating Offshore Wind Turbines (FOWT) with Oscillating Water Column (OWCs) devices arise as a promising solution for hybrid renewable energy production. In these systems, OWC modules are employed not only for wave energy generation but also for FOWT stabilization, cost-efficiency and prognosis. Nevertheless, analyzing and understanding the aero-hydro-servo-elastic floating structure control performance composes an intricate and challenging task. Even more given the dynamical complexity increase that involves the incorporation of OWCs within the FOWT platform. In this regard, although some time and frequency domain models have been developed, they are complex, computationally inefficient and not suitable for neither real-time nor feedback control. In this context, this work presents a novel control-oriented regressive model for hybrid OWC-FOWT platforms. The main objective is to take advantage of the predictive and forecasting capabilities of the deep-layered artificial neural networks (ANN), jointly with their computational simplicity, to develop a feasible control-oriented and lightweight model compared to the aforementioned complex dynamical models. In order to achieve this objective, a deep-layered ANN model has been designed and trained to match the hybrid platform’s structural performance. Then, the obtained scheme has been benchmarked against standard Multisurf-Wamit-FAST 5MW FOWT output data for different challenging scenarios in order to validate the model. The results demonstrate the adequate performance and accuracy of the proposed ANN control-oriented model, composing a great alternative for complex non-linear models traditionally used and allowing the implementation of advanced control schemes in a computationally convenient, straightforward and easy way.
Identification of hub genes and therapeutic siRNAs to develop novel adjunctive therapy for Duchenne muscular dystrophy
Authors: Li Na , Zhikai Xiahou , Li Zhuo , Zhang Zilian , Song Yafeng 
Published: October 2022

Abstract
Objective. Duchenne muscular dystrophy (DMD) is a devastating X-linked neuromuscular disorder caused by various defects in the dystrophin gene and still no universal therapy. This study aims to identify the hub genes unrelated to excessive immune response but responsible for DMD progression and explore therapeutic siRNAs, thereby providing a novel treatment.
Methods. Top ten hub gene for DMD were identified from GSE38417 dataset by using GEO2R and PPI network based on Cytoscape analysis. The hub genes unrelated to excessive immune response were identified by GeneCards, and their expression was further verified in mdx and C57 mice at 2 and 4 months (M) by (RT-q) PCR and western bloting. Therapeutic siRNAs were deemed as those that could normalized the expression of the validated hub genes in transfected C2C12 cell.
Results. 855 up-regulated and 324 down-regulated DEGs were screened from GSE38417 dataset. Five of the top 10 hub genes were considered as the candidate genes unrelated to excessive immune response, and three of these candidates were consistently and significantly up-regulated in mdx mice at 2M and 4 M when compared with age-matched C57 mice, including Col1a2, Fbn1and Fn1. Furthermore, the three validated up-regulated candidate genes can be significantly down-regulated by three rational designed siRNA (p<0.0001), respectively.
Conclusion. COL1A2, FBN1, and FN1 may be novel biomarkers for DMD, and the siRNAs designed in our study were help to develop adjunctive therapy for Duchenne muscular dystrophy.
The effects of repeated therapeutic administration of artesunate-amodiaquine and artemether-lumefantrine on haematological markers in healthy mice.
Authors: Audu David , Ajoke  , B Patel Vinood , F.M Mshelbwala , Adewunmi B Idowu 
Published: October 2022

Abstract
Artemisinin Combination Therapy (ACT) is readily available in malaria-endemic nations, leading to repeated usage of the drug by undiagnosed persons. Repeated ACT therapy in non-infected individuals may affect blood cells. In this study, we examined how repeated therapeutic use of artesunate-amodiaquine (A/A) and artemether-lumefantrine (A//L) when not infected could alter haematological parameters. This experiment used 100 male BALB/c mice randomly distributed into 5 groups: non-infected and Plasmodium berghei NK65 infected groups treated with therapeutic dosages of A/L and A/A 1X, 2X, 3X, 4X, 5X and 6X consecutive times and the control group. Blood was collected for haematological analysis one week after 1X, 2X, 3X and 6X exposure periods. The findings show Packed cell Volume (PCV), Haemoglobin (Hb), and Red Blood Cell counts (RBC) were reduced (p>0.05) non-significantly in the non-infected group treated with either A/L or A/A six consecutive times compared to control and Infected group. In addition, white blood cell count (WBC) increased in infected and non-infected mice treated with A/L or A/A 1X, 2X, 3X, and 6X times, with a significant increase in non-infected mice treated with A/L (p < 0.01) and A/A (p < 0.001) for three consecutive times. The increase in WBC was caused predominantly by Lymphocyte rise, but a decrease in neutrophils was observed. These findings indicate that repeated therapeutic use of A/L and A/A without infection could result in a haematological alteration. Therefore, continuous efforts are needed to enlighten the public on the need to repeatedly screen for malaria parasites before every drug usage.
Aberrant adenosine signaling in patients with focal cortical dysplasia
Authors: Guo Mengyi , Zhang Jing , Wang Jing , Wang Xiongfei , Gao Qing , Tang Chongyang , Deng Jiahui , Xiong Zhonghua , Kong Xiangru , Guan Yuguang , Zhou Jian , Boison Detlev , Luan Guoming , Li Tianfu 
Published: October 2022

Abstract

Focal cortical dysplasia (FCD), a common malformation of cortical development, is frequently associated with pharmacoresistant epilepsy in both children and adults. Adenosine is an inhibitory modulator of brain activity and a prospective anti-seizure agent with potential for clinical translation. Our previous results demonstrated that the major adenosine-metabolizing enzyme adenosine kinase (ADK) was upregulated in balloon cells (BCs) within FCD type IIB lesions, suggesting that dysfunction of the adenosine system is implicated in the pathophysiology of FCD. In our current study, we therefore performed a comprehensive analysis of adenosine metabolism and signaling in surgically resected cortical specimens from patients with FCD type I and type II via immunohistochemistry and immunoblot analysis. Adenosine metabolism was assessed by quantifying the levels of the key enzymes of adenosine metabolism, i.e., ADK, adenosine deaminase (ADA), and 5’-ectonucleotidase (CD73). Adenosine signaling was assessed by quantifying the levels of adenosine A2Areceptor (A2AR) and putative downstream mediators of adenosine, namely, glutamate transporter-1 (GLT-1) and mammalian target of rapamycin (mTOR). Within lesions in FCD specimens, we found that the adenosine-metabolizing enzymes ADK and ADA, as well as the adenosine-producing enzyme CD73, were upregulated. We also observed an increase in A2AR expression, as well as a decrease in GLT-1 levels and an increase in mTOR levels, in FCD specimens compared with control tissue. These results suggest that dysregulation of the adenosine system is a common pathologic feature of both FCD type I and type II. The adenosine system might therefore be a therapeutic target for the treatment of epilepsy associated with FCD.
A Novel Approach for Brake Emission Estimation based on Traffic Microsimulation, Vehicle System Dynamics, and Machine Learning Modeling
Authors: Rahimi Mostafa , Bortoluzzi Daniele , Candeo Stefano , Biral Francesco , Wahlström Jens 
Published: October 2022

Abstract
Brake wear is known as the primary source of traffic-related non-exhaust particle generation. Its generation rate is influenced by parameters at different levels: subsystem (type of brakes, pads, materials, etc.), system (vehicles’ dynamics, driving style etc.) and suprasystem (road geometries, traffic parameters, etc.). At the subsystem level, we proposed a neural network brake emission modeling, trained and validated through emission data collected from a reduced-scale dynamometer. At the system level, a model of a car dynamics was developed to calculate the wheels’ brake torques and angular velocities. At the suprasystem level, the traffic behavior in a sensitive urban area was characterized experimentally and simulated in a traffic microsimulation software. The vehicle traffic-based records were used to calculate the vehicle dynamic quantities, converted into brake emission through the neural network. To examine the overall traffic impacts on brake emission, the total number of brake wear particles was estimated regarding the route choice in the sensitive area and in the whole transportation network. The findings of this study showed significant impacts of brake wear on air pollution. The ground-truth brake emission estimation in a real area provides fundamental information to the decision-makers to better insight into the rate of non-exhaust emissions generation.
Investigation of antibiotic resistant vibrios associated with shrimp (Litopenaeus vannamei) farms
Authors: Das Prabina , Swaminathan T. Raja , Mohandas Sowmya P , Anjana J. C , Manjusha K. , Preena P. G. 
Published: October 2022

Abstract

For the sustainable farming of disease-free and healthy shrimps, antimicrobial usage is frequent nowadays in shrimp-cultured system. This could lead to the emergence of severe antimicrobial resistance (AMR) in the whole ecosystem. Considering the serious impact of global AMR, the present study was focused to investigate the prevalence of antimicrobial resistant vibrios among infected shrimps (Litopenaeus vannamei) from two brackish-water cultured farms. Diverse species of vibrios viz. V. alginolyticus, V. parahaemolyticus, V. cholerae, V. mimicus and V. fluvialis and other isolates such as Aeromonas hydrophila, A. salmonicida and Shewanella algae were also recovered from the shrimps on TCBS medium.Shannon wiener diversity index, H’ (loge) was found to be 1.506 for the isolates from farm 1 and 1.69 for those from farm 2. V. alginolyticus was found to be the most resistant isolate by showing MAR index of 0.60 followed by V. mimicus (0.54) and V. parahaemolyticus(0.42). Among the 35 antibiotics of 15 different classes tested, tetracyclines, beta lactams and cephalosporins were found as the most resistant antibiotic classes. An increased altered resistance phenotype and a drastic change in MAR index were noticed after plasmid curing. Since the studied shrimp samples are of significance in food sector and plasmid-borne AMR is evident among the isolates, public health is also alarming. This baseline information will help the authorities to curb the antimicrobial use and pave the way for establishing new alternative strategies by undertaking multidimensional “One-Health” approach.
Effect of different protein diet on the prognosis of children with Henoch-Schönlein Purpura
Authors: LI HUI , FENG KAI 
Published: October 2022

Abstract
Introduction

The aim of this study was to explore the association of different protein diet with the recurrence/recurrent rash of Henoch-Schönlein purpura (HSP) and to analyze the potential risk factors for recurrence of HSP.

Methods

A total of 72 children with HSP treated in Beijing Children's Hospital from January to June 2021 were collected retrospectively. According to the different protein diets, the children were divided into an animal protein group with 32 children and a plant protein group with 40 children. The clinical data of the children were collected, including general data, times of skin rash recurrence, the incidence of HSP recurrence, and the incidence of renal lesions. The daily protein intake of the children was recorded by questionnaire and followed up for 8 weeks after discharge.

Results

There were no statistical difference between animal protein group and plant protein group in times of skin rash recurrence, the incidence of HSP recurrence, and the incidence of renal lesions of children (p > 0.05). However, the protein intake of animal protein group was significantly higher than that of plant protein group (p < 0.05), which did not affect the recurrence of HSP (p > 0.05). In addition, respiratory infection at the beginning of the disease was potential risk factors for the recurrence of HSP in children (p < 0.05).

Conclusions

There is no significant association between animal or plant protein diet and the recurrence of HSP or skin rash. Timely avoidance of infection after cure may help to reduce the recurrence rate of HSP in children.
Prudent walk in dimension six and higher
Authors: Heydenreich Markus , Taggi Lorenzo , Torri Niccolò 
Published: October 2022

Abstract
We study the high-dimensional uniform prudent self-avoiding walk, which assigns equal prob- ability to all nearest-neighbor self-avoiding paths of a fixed length that respect the prudent condi- tion, namely, the path cannot take any step in the direction of a previously visited site. We prove that the prudent self-avoiding walk converges to Brownian motion under diffusive scaling if the dimension is large enough. The same result is true for weakly prudent walk in dimension d > 5.
A challenging property of the high-dimensional prudent walk is the presence of an infinite- range self-avoidance constraint. Interestingly, as a consequence of such a strong self-avoidance constraint, the upper critical dimension of the prudent walk is five, and thus greater than for the classical self-avoiding walk.
Mathematics Subject Classification: 82B41 · 60G50
Studying the modal coupling effect on the FRF response to the non-classically damped structures
Authors: Suleiman Hisham , Afra Hamid , Abdeddaim Mahdi , Bouzerd Hamoudi 
Published: October 2022

Abstract
This research aims to study the effect of neglecting modal decoupling due to Rayleigh's lightly non-classical damping hypothesis when investigating the frequency response functions of the non-classically damped systems. Also, the present work examines how modal coupling impacts the energy transfer between the different damped modes of these systems. This study considers the modal analysis of non-classically damped linear dynamic systems by introducing damping devices into multi-degree freedom frame systems. This study presents three methods for solving the quadratic eigenvalue problem of the non-classically damped systems: The state-space method for obtaining the exact solution, the lightly non-classical method for the undamped complex eigenvalue problem, and Adhikari's iteration method to solve the original space problem. The results of decoupling the modes of the non-classically damped system using the modal assurance coefficient and plotting the frequency and phase responses are discussed. This study proposes an example of a multi-degree freedom frame system with an introduced external damper with three variations to illustrate the modal decoupling of non-classically damped systems.