bluuebunny/crossref_metadata_2025_split huggingface dataset:
Baseline study for improving diagnostic stewardship at secondary health care facilities in Nigeria
Authors: Egwuenu Abiodun , Ejikeme Adaora , Tomczyk Sara , von Laer Anja , Ayobami Olaniyi , Odebajo Oluwaseun , Akhibi Samuel , Agulanna Constance , Osagie Osayande , Inweregbu Ugochi Stellamaris , Yahaya Ridwan , Okwor Tochi , Dada-Adegbola Hannah , Ajayi Ikeoluwapo , Olorukooba Abdulhakeem , Eckmanns Tim , Ochu Chinwe Lucia , Ihekweazu Chikwe 
Published: May 2022

Abstract
Background
Blood culture diagnostics are critical tools for sepsis management and antimicrobial resistance (AMR) surveillance. A baseline study was conducted to assess reported sepsis case finding, blood culture diagnostics, antimicrobial susceptibility testing (AST) and antimicrobial use at secondary health care facilities to inform the development of diagnostic stewardship improvement strategies in Nigeria.

Methods
A cross-sectional online survey was conducted among 25 public secondary health care facilities in Abuja, Federal Capital Territory (FCT) and Lagos State in Nigeria to evaluate the capacity for pathogen identification and AST. Data were then prospectively extracted on all patients with reported suspected sepsis from electronic medical records from selected departments at two facilities in the Federal Capital Territory from October 2020 to May 2021 to further assess practices concerning sepsis case-finding, clinical examination findings, samples requested, and laboratory test results. Data were descriptively analysed, and a multivariate logistic regression analysis was conducted to determine factors associated with blood culture requests.

Results
In the online survey, 32% (8/25) of facilities reported performing blood cultures. Only one had access to a clinical microbiologist, and 28% (7/25) and 4% (1/25) used standard bacterial organisms for quality control of media and quality control strains for AST, respectively. At the two facilities where data abstraction was performed, the incidence of suspected sepsis cases reported was 7.1% (2924/41066). A majority of these patients came from the paediatrics department and were outpatients, and the median age was two years. Most did not have vital signs and major foci of infection documented. Blood cultures were only requested for 2.7% (80/2924) of patients, of which twelve were positive for bacteria, mainly Staphylococcus aureus. No clinical breakpoints were used for AST. Inpatients (adjusted odds ratio [aOR]: 7.5, 95% CI: 4.6–12.3) and patients from the urban health care facility (aOR:16.9, 95% CI: 8.1–41.4) were significantly more likely to have a blood culture requested.

Conclusion
Low blood culture utilisation remains a key challenge in Nigeria. This has implications for patient care, AMR surveillance and antibiotic use. Diagnostic stewardship strategies should focus on improving access to clinical microbiology expertise, practical guidance on sepsis case finding and improving blood culture utilisation and diagnostics.
Anti-infammatory and antioxidant activities of acteoside isolated from Acanthus ilicifolius var. xiamenensis
Authors: Zhang Yifan , Shen Jinhuang , Ma Xinhua , Yao Mingshuang , Zhang Yonghong , Cao Dairong 
Published: May 2022

AbstractAcanthus ilicifolius var. xiamenensis (Acanthaceae), a mangrove found in southeastern China, is an herb with strong antiinflammatory property. Phytochemical study of the mangrove showed that the plant has a high content of phenylethanol glycoside acteoside (AC). In this research, the anti-inflammatory activity of acteoside on dextran sodium sulphate (DSS)-mediated mouse ulcerative colitis model were studied. For DSS- mediated colitis in mice, AC can reduce weight loss and DAI score in UC mice, suppress colon shortening and alleviate colon pathological injury. Moreover, AC treatment notably up-regulates IL-10, down-regulates the levels of IL-1β and TNF-α, and inhibits the protein expression of JAK2/STAT3, NF-κB p65, IKKα/β and IKB of colon. In addition, after AC treatment, the level of MDA and NO in colonic tissue were remarkably decreased, while the levels of GSH, SOD, and Nrf2 and HO-1 protein expression levels were significantly increased. These results indicate that AC can activate the Nrf2 signaling pathway by inhibiting the JAK/STAT, iNOS/eNOS and NF-κB signaling cascades, enhance the intestinal barrier function, and effectively reduce DSS-induced UC in mice.
Differential gene expression of ABCG2, SLC22A12, IL-1β, and ALPK1 in peripheral blood leukocytes of primary gout patients with hyperuricemia and their comorbidities: a case–control study
Authors: Natsuko Paniagua-Díaz , Laura Sanchez-Chapul , Denise Clavijo-Cornejo , Lucio Ventura-Ríos , Carlos Aguilar-Salinas , Fausto Sanchez-Muñoz , Ambar López-Macay 
Published: May 2022

Abstract
Background
The ABCG2, SLC22A12, and ALPK1 genes have been strongly associated with dysfunction of urate metabolism in patients with gout, but it is unknown how these transporters are expressed in patients with acute or chronic gout. Our objectives were to: (a) analyze the gene expression of urate transporters and of inflammation genes in peripheral blood from gout patients and controls; (b) determine whether the metabolic profile of gout patients can influence the gene expression profile and the expression of urate transporters, ABCG2 and SLC22A12, and inflammation molecules, ALPK1 and IL-1β, in peripheral blood leukocytes from gout patients; (c) compare them with their metabolic profile and the gene expression of people without gout and without hyperuricemia.

Methods
A total of 36 chronic and acute patients and 52 controls were recruited, and ABCG2, SLC22A12, IL-1β, and ALPK1 gene expression was evaluated by quantitative real-time PCR. Correlations of gene expression with clinical and laboratory parameters of patients were also analyzed.

Results
IL-1β was significantly increased in peripheral blood mononuclear cells (PBMCs) of patients compared with their polymorphonuclear leukocytes white blood cells (PMNLs, p < 0.05). A significant increase in ABCG2 and IL-1β was found in PMNLs from patients compared to controls (p < 0.05). Correlations of gene expression in patients were found with levels of serum uric acid (sUA), serum creatinine, C-reactive protein (CRP), triglycerides, body mass index (BMI), kidney disease, hypertension, and metabolic syndrome.

Conclusions
Our data suggest that leukocytes of patients respond to the presence of hyperuricemia and comorbidities, expressing ABCG2 and IL-1β genes differentially compared to normouricemic and nondisease states. Hyperuricemia, dyslipidemia, and obesity probably stimulate the differential gene expression of peripheral blood leukocytes (neutrophils and monocytes), even in an asymptomatic state.
Fecal microbiota transfer between young and aged mice reverses hallmarks of the aging gut, eye, and brain
Authors: Parker Aimée , Romano Stefano , Ansorge Rebecca , Aboelnour Asmaa , Le Gall Gwenaelle , Savva George M. , Pontifex Matthew G. , Telatin Andrea , Baker David , Jones Emily , Vauzour David , Rudder Steven , Blackshaw L. Ashley , Jeffery Glen , Carding Simon R. 
Published: May 2022

Abstract
Background
Altered intestinal microbiota composition in later life is associated with inflammaging, declining tissue function, and increased susceptibility to age-associated chronic diseases, including neurodegenerative dementias. Here, we tested the hypothesis that manipulating the intestinal microbiota influences the development of major comorbidities associated with aging and, in particular, inflammation affecting the brain and retina.

Methods
Using fecal microbiota transplantation, we exchanged the intestinal microbiota of young (3 months), old (18 months), and aged (24 months) mice. Whole metagenomic shotgun sequencing and metabolomics were used to develop a custom analysis workflow, to analyze the changes in gut microbiota composition and metabolic potential. Effects of age and microbiota transfer on the gut barrier, retina, and brain were assessed using protein assays, immunohistology, and behavioral testing.

Results
We show that microbiota composition profiles and key species enriched in young or aged mice are successfully transferred by FMT between young and aged mice and that FMT modulates resulting metabolic pathway profiles. The transfer of aged donor microbiota into young mice accelerates age-associated central nervous system (CNS) inflammation, retinal inflammation, and cytokine signaling and promotes loss of key functional protein in the eye, effects which are coincident with increased intestinal barrier permeability. Conversely, these detrimental effects can be reversed by the transfer of young donor microbiota.

Conclusions
These findings demonstrate that the aging gut microbiota drives detrimental changes in the gut–brain and gut–retina axes suggesting that microbial modulation may be of therapeutic benefit in preventing inflammation-related tissue decline in later life.

Graphical abstract
Social insights on the implementation of One Health in zoonosis prevention and control: a scoping review
Authors: He Junyi , Guo Zhaoyu , Yang Pin , Cao Chunli , Xu Jing , Zhou Xiaonong , Li Shizhu 
Published: May 2022

Abstract
Background
The One Health (OH) concept has been promoted widely around the globe. OH framework is expected to be applied as an integrated approach to support addressing zoonotic diseases as a significant global health issue and to improve the efficiency and effectiveness of zoonosis prevention and control. This review is intended to overview the social impact of the implementation of OH on zoonosis prevention and control.

Methods
A scoping review of studies in the past 10 years was performed to overview the integration feature of OH in zoonosis prevention and control and the social impacts of OH. PubMed and Web of Science were searched for studies published in English between January 2011 and June 2021. The included studies were selected based on predefined criteria.

Results
Thirty-two studies were included in this review, and most of them adopted qualitative and semi-qualitative methods. More than 50% of the studies focused on zoonosis prevention and control. Most studies were conducted in low- and middle-income countries in Africa and Asia. Applying OH approach in diseases control integrates policymakers, stakeholders, and academics from various backgrounds. The impact of OH on economic is estimated that it may alleviate the burden of diseases and poverty in the long term, even though more financial support might be needed at the initial stage of OH implementation. OH implementation considers social and ecological factors related to zoonosis transmission and provides comprehensive strategies to assess and address related risks in different communities according to regions and customs.

Conclusions
Based on reviewed literature, although there seems to be a lack of guidelines for assessing and visualizing the outcomes of OH implementation, which may limit the large-scale adoption of it, evidence on the contributions of implementing OH concepts on zoonosis prevention and control indicates long-term benefits to society, including a better integration of politics, stakeholders and academics to improve their cooperation, a potential to address economic issues caused by zoonosis, and a comprehensive consideration on social determinants of health during zoonosis prevention and control.
The emerging landscape of exosomal CircRNAs in solid cancers and hematological malignancies
Authors: Zhou Qinfeng , Xie Dacheng , Wang Rong , Liu Lianfang , Yu Yue , Tang Xinyi , Hu Yongxian , Cui Dawei 
Published: May 2022

AbstractCircular RNAs (circRNAs) are a type of recently discovered noncoding RNA. They exert their biological functions by competitively binding to microRNAs (miRNAs) as miRNA sponges, promoting gene transcription and participating in the regulation of selective splicing, interacting with proteins and being translated into proteins. Exosomes are derived from intracavitary vesicles (ILVs), which are formed by the inward budding of multivesicular bodies (MVBs), and exosome release plays a pivotal role in intercellular communication. Accumulating evidence indicates that circRNAs in exosomes are associated with solid tumor invasion and metastasis. Additionally, emerging studies in the last 1 ~ 2 years have revealed that exosomal circRNA also have effect on hematological malignancies. In this review, we outline the properties and biological functions of circRNAs and exosomes. In particular, we summarize in detail the mechanism and roles of exosomal circRNAs and highlight their application as novel biomarkers in malignant tumors.
New archaeobotanical evidence for Medicago from the Astana Cemetery in Turpan, Xinjiang
Authors: Chen Tao , Wang Bo , Jiang Hongen 
Published: May 2022

Abstract
The study of crop dispersal and their cultivation technique communication can provide a valuable insight into the history of cultural exchange in ancient Eurasia. Previous studies have focused predominantly on the cereals, with few being carried out on fruit, vegetable and forage crops. This paper reports on legume remains found at three tombs of the Astana Cemetery, Turpan, Xinjiang, which dated to the Jin and Tang dynasties (about third to ninth centuries). The exceptional state of preservation of the desiccated remains allowed their unambiguous determination to species level. By comparing the morphological characteristics of the Medicago remains with published references, they were ascribed to Medicago sativa and Medicago lupulina. This is the first archaeobotanical evidence of M. lupulina in China and the earliest relevant physical material so far found. In addition, combined with the record of unearthed documents, our study shed new light on the early history of M. sativa cultivation and use in Turpan and further underlined its important role in cultural exchange between the West and the East in antiquity.
Improved cost-sensitive representation of data for solving the imbalanced big data classification problem
Authors: Fattahi Mahboubeh , Moattar Mohammad Hossein , Forghani Yahya 
Published: May 2022

AbstractDimension reduction is a preprocessing step in machine learning for eliminating undesirable features and increasing learning accuracy. In order to reduce the redundant features, there are data representation methods, each of which has its own advantages. On the other hand, big data with imbalanced classes is one of the most important issues in pattern recognition and machine learning. In this paper, a method is proposed in the form of a cost-sensitive optimization problem which implements the process of selecting and extracting the features simultaneously. The feature extraction phase is based on reducing error and maintaining geometric relationships between data by solving a manifold learning optimization problem. In the feature selection phase, the cost-sensitive optimization problem is adopted based on minimizing the upper limit of the generalization error. Finally, the optimization problem which is constituted from the above two problems is solved by adding a cost-sensitive term to create a balance between classes without manipulating the data. To evaluate the results of the feature reduction, the multi-class linear SVM classifier is used on the reduced data. The proposed method is compared with some other approaches on 21 datasets from the UCI learning repository, microarrays and high-dimensional datasets, as well as imbalanced datasets from the KEEL repository. The results indicate the significant efficiency of the proposed method compared to some similar approaches.
Experimental simulations of shock textures in BCC iron: implications for iron meteorites
Authors: Ohtani Eiji , Sakurabayashi Toru , Kurosawa Kosuke 
Published: May 2022

AbstractNeumann band in iron meteorites, which is deformation twins in kamacite (Fe–Ni alloy), has been known to be a characteristic texture indicating ancient collisions on parent bodies of meteorites. We conducted a series of shock recovery experiments on bcc iron with the projectile velocity at 1.5 km/s at various initial temperatures, room temperature, 670 K, and 1100 K, and conducted an annealing experiment on the shocked iron. We also conducted numerical simulations with the iSALE-2D code to investigate peak pressure and temperature distributions in the nontransparent targets. The effects of pressure and temperature on the formation and disappearance of the twins (Neumann band) were explored based on laboratory and numerical experiments. The twin was formed in the run products of the experiments conducted at room temperature and 670 K, whereas it was not observed in the run product formed by the impact at 1100 K. The present experiments combined with the numerical simulations revealed that the twin was formed by impacts with various shock pressures from 1.5–2 GPa to around 13 GPa. The twin in iron almost disappeared by annealing at 1070 K. The iron meteorites with Neumann bands were shocked at this pressure range and temperatures at least up to 670 K, and were not heated to the temperatures above 1070 K after the Neumann band formation.
177Lu-DOTA-0-Tyr3-octreotate infusion modeling for real-time detection and characterization of extravasation during PRRT
Authors: Mazzara Christophe , Salvadori Julien , Ritzenthaler Florian , Martin Simon , Porot Clémence , Imperiale Alessio 
Published: May 2022

Abstract
Purpose
Given the recent and rapid development of peptide receptor radionuclide therapy (PRRT), increasing emphasis should be placed on the early identification and quantification of therapeutic radiopharmaceutical (thRPM) extravasation during intravenous administration. Herein, we provide an analytical model of 177Lu-DOTA0-Tyr3-octreotate (Lutathera®) infusion for real-time detection and characterization of thRPM extravasation.

Methods
For 33 Lutathera®-based PRRT procedures using the gravity infusion method, equivalent dose rates (EDRs) were monitored at the patient’s arm. Models of flow dynamics for nonextravasated and extravasated infusions were elaborated and compared to experimental data through an equivalent dose rate calibration. Nonextravasated infusion was modeled by assuming constant volume dilution of 177Lu activity concentration in the vial and Poiseuille-like laminar flow through the tubing and patient vein. Extravasated infusions were modeled according to their onset times by considering elliptically shaped extravasation region with different aspect ratios.

Results
Over the 33 procedures, the peak of the median EDR was reached 14 min after the start of the infusion with a value of 450 µSv h−1. On the basis of experimental measurements, 1 mSv h−1 was considered the empirical threshold for Lutathera® extravasation requiring cessation of the infusion and start again with a new route of injection. According to our model, the concentration of extravascular activity was directly related to the time of extravasation onset and its duration, a finding inherent in the gravity infusion method. This result should be considered when planning therapeutic strategy in the case of RPM extravasation because the local absorbed dose for β-emitters is closely linked to activity concentration. For selected EDR values, charts of extravasated activity, volume, and activity concentration were computed for extravasation characterization.

Conclusion
We proposed an analytical model of Lutathera® infusion and extravasation (gravity method) based on EDR monitoring. This approach could be useful for the early detection of thRPM extravasation and for the real-time assessment of activity concentration and volume accumulation in the extravascular medium.
Prediction of the short-term efficacy of anti-VEGF therapy for neovascular age-related macular degeneration using optical coherence tomography angiography
Authors: Jia Huixun , Lu Bing , Zhao Zhi , Yu Yang , Wang Fenghua , Zhou Minwen , Sun Xiaodong 
Published: May 2022

AbstractBackgroundTo evaluate whether the specific choroidal neovascularization (CNV) characteristics measured using optical coherence tomography angiography (OCTA) can predict the 6-month prognosis of neovascular age-related macular degeneration (nAMD) after anti-vascular endothelial growth factor (anti-VEGF) therapy.MethodsPatients with type 1, type 2, or mixed-type neovascularization (NV) were prospectively included. Participants underwent an initial loading phase of three consecutive monthly intravitreal injections of Conbercept (0.5 mg) and were switched to a pro re nata (PRN) treatment strategy. OCTA images were evaluated for eyes that underwent follow-up assessments for more than 6 months. CNV lesions were manually segmented, and the CNV area, vessel area, greatest vascular caliber (GVC), and greatest linear dimension (GLD) were compared between responders and non-responders. Two masked graders independently measured the above-mentioned parameters using OCTA, and consistency was assessed using the intraclass correlation coefficient (ICC) values. Multiple logistic regression analysis was performed to evaluate the effect of a 3-month change in the CNV area, GLD, and GVC on the 6-month response to anti-VEGF agents.ResultsAmong the 60 eyes of 60 patients with nAMD, 39 were responders and 21 were non-responders. The proportion of CNV types was significantly different between responders and non-responders (P = 0.009). Patients with type 2 or mixed NV seemed more likely to respond to the treatment (28.2%vs.0.0%, and 30.8%vs.23.8%, respectively). The change in GVC showed a significant difference between responders (− 4.98 ± 17.17 μm) and non-responders (11.01 ± 14.10 μm) after three monthly intravitreal anti-VEGF injections. Multiple logistic regression analysis showed that only the change in GVC remained significant after controlling for baseline GVC, injection number, and CNV type (adjusted OR = 1.083;P = 0.008).ConclusionsType 2 and mixed-type NV were significantly associated with a better response to anti-VEGF therapy. Changes in GVC after 3 months of treatment were significantly associated with a response to anti-VEGF therapy at 6 months.