bluuebunny/crossref_metadata_2025_split huggingface dataset:
In-home environment for happy ageing in Thailand, a middle- income country in Asia
Authors: Pekalee Alongkorn , Gray Rossarin Soottipong 
Published: April 2022

Abstract
Background
The fact that housing can play a critical role in maintaining the health and independence related to happiness of the older population has been studied in more developed countries. However, research on the effect of housing conditions on happiness is rare in less developed countries. This study aimed to construct and test a structural equation model describing the structural relationship among personal aspects (living alone and physical disability), in-home environment, and happiness, controlling for other generally-acknowledged determinants such as age, gender, marital status, education and income, among older adults in Thailand.
Methods
The data on the population age 75 years or over were extracted from the 2017 Survey of Older Persons in Thailand, carried out by the Thai National Statistical Office (n = 7,829). Data were analyzed using AMOS Version 18.
Results
The median age of the sample population was 79. Almost 60 percent were women. About one in six (16.7 percent) lived alone and three out of five had no physical disability. Housing conditions, particularly in the sleeping place and toilet/bathroom needed to be improved. The structural equation model showed a good fit with the data, χ2 = 302.397 (p < .001), goodness of fit index (GFI) = .964, normed fit index (NFI) = .959, comparative fit index (CFI) = .964, and root mean square error of approximation (RMSEA) = .10. In-home environment had a significantly positive effect on older adults’ happiness (path coefficient = .235, p < .001). Physical disability had a significantly positive effect on in-home environment (path coefficient = .113, p < .001).
Conclusions
Interventions to improve happiness of older adults, particularly those with physical disability, should aim to adapt their housing, including sleeping place and toilet design.
Community engagement and context in setting up a multi-faceted longitudinal intervention in rural India: The Healthy Life Trajectories Initiative
Authors: Prabhu Manohar Prasad , Suryanarayana Kumar Gavali , Ghattu Krishnaveni , Adiga Nagabharana TK , Joseph Shama , Rizwana Azeez , Bhat Gurudath , Naika Girisha , Ninganayaka Susheela , Mathews Stephen , Shah Prakeshkumar , Sellen Daniel , Kalyanaraman Kumaran , Nagaraj Kiran , Fall Caroline HD 
Published: April 2022

Abstract
The Healthy Life Trajectories Initiative programme is a ten-year joint initiative consisting of four separate but harmonised intervention studies in Mysore (India), Johannesburg (South Africa), Shanghai (China) and two provinces in Canada. They will test evidence-based multi-faceted interventions spanning from preconception into the postnatal period, to improve maternal, infant and child health, including cardiometabolic and neurocognitive well-being. Similar to the other studies, the India study was based on developmental origins of health and disease principles. However, it had to be adapted to fit in with the local socio-cultural context and government policy. We considered the contextual factors in our setting and undertook extensive engagement and formative work with the local community to develop the intervention package and plan delivery methods. This article describes the process we adopted to set up the India project, develop the intervention package and delivery methods.
Clinical Subtype, Treatment Response, and Survival in De Novo and Recurrent Metastatic Breast Cancer
Authors: File Danielle , Pascual Tomas , Deal Allison , Wheless Amy , Perou Charles , Dees E. Claire , Carey Lisa Anne 
Published: April 2022

Abstract
Purpose. This study evaluated whether patients with de novo metastatic breast cancer (MBC) have superior outcomes compared to those with recurrent MBC in a contemporary treatment era and examined factors related to outcome differentials.Methods. Using an institutional database, we examined patient and tumor characteristics, treatment response, and outcome among 232 patients with de novo and 612 patients with recurrent MBC diagnosed between 2011-2017.Results. De novo MBC had 9-month (m) longer overall survival (OS) than recurrent MBC (36.4 vs 27.4m, p<0.001). Contributions to this difference included nearly two-fold more HER2-positive (29.3% vs 15.2%) and significantly fewer triple negative breast cancers (20.3% vs 32.4%, both p<0.001) in de novo compared with recurrent MBC cohorts. Stratified by clinical subtype, progression-free survival (PFS) on first-line therapy was significantly longer in de novo MBC in all but the triple negative subtype: 25.5 vs 11.6m (p<0.001) among 390 patients with hormone receptor-positive, HER2-negative, 11.4 vs 5.4m (p=0.002) among 142 patients with HER2-positive, and 4.0 vs 3.0m (p=0.121) among 162 with triple negative MBC. In multivariable analysis, de novo status remained independently associated with improved OS (hazard ratio 0.63, 95% CI 0.49-0.80), regardless of subtype and other features. Conclusion. Patients with de novo MBC have better outcomes than those with recurrent MBC. Differences in clinical subtype and response to therapy in the metastatic setting contribute to, but do not fully explain, this difference. Longer PFS to first-line therapy in de novo MBC suggests biologic differences compared to recurrent MBC, which may be intrinsic or due to acquired resistance from treatment for prior localized breast cancer in recurrent disease.
Red foxes (Vulpes vulpes) are exposed to high diversity of Borrelia burgdorferi sensu lato species infecting fox-derived Ixodes ticks in west-central Poland
Authors: Wodecka Beata , Michalik Jerzy , Grochowalska Renata 
Published: April 2022

Abstract

Background: The red fox, Vulpes vulpes is the most widely distributed terrestrial carnivore species in Europe, which is parasitized by exophilic as well as nidicolous Ixodes ticks. However, the role of these canids and their tick fauna in the maintenance of Borrelia burgdorferi sensu lato (s.l.) is still insufficiently understood. Results: A total of 1,583 ticks were removed from ears of 120 infested animals and were identified to species using a nested PCR targeting the ITS2 and cox1 fragments of Ixodes DNA. Ixodes kaiseri prevailed (76%), followed by I. canisuga (12%), I. ricinus (10%), and I. hexagonus (2%). In total, 32.4% of 943 ticks yielded Borrelia DNA and ten species of B. burgdorferi s.l. complex were identified. Borrelia garinii and B. afzelii composed 70% of all infections. The other eight species included B. americana, B. bissettiae, B. burgdorferi sensu stricto (s.s.), B. californiensis, B. carolinensis, B. lanei, B. spielmanii, and B. valaisiana. Analysis of 229 foxes showed that 25% were infected with B. burgdorferi s.l. Borrelia garinii was detected in 91% of the infected animals, including 31% of mixed infections with B. afzelii, the second most prevalent species, followed by B. spielmanii. Conclusions: The clear predominance of B. garinii in PCR-positive animals and infected larval ticks (38.1%), suggests that this spirochete and B. afzelii are preferentially associated with these wild canids. Although red foxes are exposed to high diversity of B. burgdorferi s.l. species found in engorged Ixodes ticks, their reservoir competence for most of them appears to be low.
dcHiC: differential compartment analysis of Hi-C datasets
Authors: Chakraborty Abhijit , Wang Jeffrey , Ay Ferhat 
Published: April 2022

Abstract
Compartmental organization of chromatin and its changes play important roles in distinct biological processes carried out by mammalian genomes. However, differential compartment analyses have been mostly limited to pairwise comparisons and with main focus on only the compartment flips (e.g., A-to-B). Here, we introduce dcHiC, which utilizes quantile normalized compartment scores and a multivariate distance measure to identify significant changes in compartmentalization among multiple contact maps. Evaluating dcHiC on three collections of Hi-C contact maps from mouse neural differentiation (n = 3), mouse hematopoiesis (n = 10) and human LCL cell lines (n = 20), we show its effectiveness and sensitivity in detecting biologically relevant differences, including those validated by orthogonal experiments. Across these experiments, dcHiC reported regions with dynamically regulated genes associated with cell identity, along with correlated changes in chromatin states, replication timing and lamin B1 association. With its efficient implementation, dcHiC not only enables high-resolution compartment analysis but also includes a suite of additional features, including standalone browser visualization, differential interaction identification, and time-series clustering. As such, it is an essential addition to the Hi-C analysis toolbox for the ever-growing number of contact maps being generated. dcHiC is freely available at https://github.com/ay-lab/dcHiC and examples from this paper can be seen at https://ay-lab.github.io/dcHiC.
Combined E velocity and E/e’ as an estimate of elevated left atrial pressure in patients with atrial fibrillation
Authors: Ma Gaigai , Fang Ligang , Lin Xue , Gao Peng , Fang Quan 
Published: April 2022

Abstract
Background: The role of transthoracic echocardiography (TTE) in assessing diastolic function in patients with atrial fibrillation (AF) has not been well characterized. We sought to determine the utility of TTE in detecting elevated left atrial pressure (LAP) in patients with chronic non-valvular AF using directly measured LAP as the reference standard.Methods: We prospectively studied 35 patients with persistent AF and preserved left ventricular ejection fraction who underwent radiofrequency ablation. LAP was measured in conjunction with trans-septal puncture at the time of catheter ablation. TTE was performed in 24 hours before ablation, peak velocity of early mitral diastolic inflow velocity (E) and mitral annular velocity (e’) were recorded.Results: E and E/e’ correlated well with mean LAP (E, r=0.560, p<0.001; E/e’avg, r=0.456, p=0.005). Using receiver operating characteristic analysis, the optimal cut-off for E was 0.91m/s (sensitivity 85%%; specificity 77%) and that for E/e’avg was 13 (sensitivity 69%; specificity 82%) to predict mean LAP>12mmHg. When combined the cut-offs of E>0.91m/s and E/e’avg>13 were used, the sensitivity and specificity of predicting elevated LAP were improved to 95% and 96%, respectively.Conclusions: In AF patients, E and E/e’ correlated well with LAP, and a combination of E and average E/e’ improved the accuracy of evaluation of elevated LAP.
The influence of source of porcine colostrum in development of early immune ontogeny in the piglet
Authors: Maciag Shaiana Salete , Bellaver Franciana Volpato , Bombassaro Gabrielly , Haach Vanessa , Morés Marcos A Zanella , Baron Lana Flávia , Coldebella Arlei , Bastos Ana Paula 
Published: April 2022

Abstract
The effect of colostrum feeding on the ontogeny of serum cytokines and immune cells in suckling piglets fed sow/gilt colostrum and milk replacer was studied. After farrowing, the born piglets were randomized into six groups: GG and SS: piglets were kept with their dam; GS: piglets were changed from gilts to sow; SG: piglets were changed from sow to gilt; GMR and SMR: piglets from gilt or sow were isolated from the dams and were bottle-fed commercial formula milk replacer. The piglets remained in the groups during the first 24 hours of life and were later returned to their respective mothers. Overall, piglets suckling sow (SS and GS) had a higher concentration of serum immunoglobulins at 24 hours, and also was associated with a rise in plasma cytokines concentration and also the greater ability of B and T cells from lymphatic organs and blood mononuclear cells to respond to mitogens. We suggest a bias towards Th1-, Th2-, Th17-cell polarizing and cytokines in the suckling period, which may be influenced by maternal immunological factors in colostrum, like parity of dam. All findings suggest a possible role of sow parity that may contribute to exerting a modulating action on immune response development.
Closed-form solution of magnetic field and magnetic gradient tensor for magnetic minerals
Authors: Ren zhengyong , zhang yuanlei , Zhong yiyuan , Pan kejia , Wu qihong , Tang jingtian 
Published: April 2022

Abstract
Magnetization is a natural property of magnetic materials which is widely used to study the ancient climate change whose footprint was recorded in rocks by palaeomagnetic data, uncover the location of unexploded ordnance, mineral deposits and gas and oil resources, and image regional crust and planet structure. An essential but very challenging task is to find analytical solutions of induced magnetic signals for general magnetic targets. In this study, for the first time, we demonstrate the existence of such solutions and explicitly give the analytical expressions of magnetic potential, magnetic field and magnetic gradient tensor for magnetic materials with polyhedral shapes and polynomial magnetization vectors. The order of magnetization polynomials can vary from linear order to arbitrary integer orders along both horizontal and vertical directions. Several synthetic and realistic iron deposit models are used to validate the accuracy and efficiency of our analytical solutions. Due to its inherent importance, our findings would have an immediate impact on magnetic explorations in earth science and have potentials in different applications.
CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex
Authors: Ma Qiang , Yang Feifei , Huang Bo , Pan Xiaojuan , Li Wei , Yu Ting , Wang Xiaolin , Ran Lingyu , Qian Kun , Li Hui , Li Haiping , Liu Yuying , Liang Ce , Ren Junwu , Zhang Yuying , Wang Shimin , Xiao Bin 
Published: April 2022

Abstract
Background
Gastric cancer (GC) is one of the most common malignant tumors in China. Circular RNAs (circRNAs) are novel non-coding RNAs with important regulatory roles in cancer progression. IGF2BP3 has been found to play oncogenic roles in various cancers including GC, while the exact mechanism of IGF2BP3 is largely unknown.
Methods
The expression of IGF2BP3 in GC was evaluated by Western Blot and bioinformatics analysis. CircRNA expression profiles were screened via IGF2BP3 RIP-seq in GC. Sanger sequencing, RNase R digestion, nucleo-plasmic separation and RNA-FISH assays were used to detect the existence and expression of circARID1A. RNA ISH assay was employed to test the expression of circARID1A in paraffin-embedded GC tissues. Moreover, the function of circARID1A on cellular proliferation was assessed by CCK-8, plate colony formation, EdU assays and GC xenograft mouse model in vivo. Furthermore, the location or binding of circARID1A, IGF2BP3 protein and SLC7A5 in GC was evaluated by RNA-FISH/IF or RNA pull-down assays.
Results
We identified a novel circRNA, circARID1A, that can bind to IGF2BP3 protein. CircARID1A was significantly upregulated in GC tissues compared with noncancerous tissues and positively correlated with tumor length, tumor volume, and TNM stage. CircARID1A knockdown inhibited the proliferation of GC cells in vitro and in vivo and circARID1A was indispensable for the oncogenic role of IGF2BP3. Mechanistically, circARID1A served as a scaffold to facilitate the interaction between IGF2BP3 and SLC7A5 mRNA, increasing SLC7A5 mRNA stability. Additionally, circARID1A was able to directly bind SLC7A5 mRNA through complementary base-pairing and then formed the circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex and promoted the proliferation of GC via AKT/mTOR pathway activation.
Conclusions
Altogether, our data suggest that circARID1A is involved in the function of IGF2BP3 and GC proliferation, and the circARID1A-IGF2BP3-SLC7A5 axis has the potential to serve as a new therapeutic target for GC.
Achieving over 70 kg m-2 h-1 evaporation rate by a 3D polyacrylamide-based hydrogel
Authors: Chen Yuying , Yang Jixiang , Jinsong Guo , Fang Fang , Bao Ruiling , Xu Linji 
Published: April 2022

Abstract
Solar water evaporation is a potential technique for clean water production with low energy input but is restricted by low evaporation rates that are usually below 5 kg m− 2 h− 1. We developed a 3D hydrogel using low-price industrial carbon nanotubes and polyacrylamide. A cotton bar, which was with a high height to diameter ratio, was used as a water supply layer. The cotton bar was covered by the hydrogel. In addition, a downwards water supply mode was applied. It is demonstrated that an evaporation rate as high as 72.3 kg m− 2 h− 1 was achieved. The evaporation rate was higher than other reported evaporation rates. Interestingly, the high evaporation rate was achieved when the ambient temperature was 25 oC and direct sunlight irritation was not necessary. Furthermore, the 3D hydrogel showed a good performance in anti-salt accumulation. The 3D hydrogel is a good candidate for clean water production, considering its high evaporation rate and cost-effectiveness as well as facile fabrication.
Radical or Modified Radical Hysterectomy does not benefit the Prognosis of Patients with StageⅡendometrial Cancer
Authors: Li Qing , Cong Rong , Wang Yuting , Kong Fanfei , Ma Jian , Li Yan , Ma Xiaoxin 
Published: April 2022

Abstract
Background: Although total hysterectomy (TH)/ radical hysterectomy (RH) + double appendectomy + staging surgery is recommended for patients with stageⅡndometrial cancer (EC), gynecologists disagree on whether to choose TH or RH/ modified radical hysterectomy (mRH) in practice. This study aims to explore whether TH or RH/mRH affect the prognosis of patients with EC.Materials and Methods: 808 stageⅡEC patients who underwent surgery were included in the retrospective analysis. The patients were grouped according to the surgical method (TH, n=434, RH/mRH, n=374) and clinical characteristics were collected. Kaplan-Meier method was used for survival analysis, and P values were calculated by log-rank test. Prognostic analysis was performed by Cox proportional hazards regression models.Results: Patients underwent RH/mRH had worse progression-free survival (PFS) (HR=2.235, 95%CI= 1.159-4.31, P=0.016), TH or RH/mRH does not affect the overall survival (OS) of patients (HR=1.511, 95%CI=0.802-2.845, P=0.201). Postoperative adjuvant therapy will improve PFS (HR=2.209, 95%CI=1.091-4.472, P=0.028). In patients received postoperative adjuvant therapy, RH/mRH is the independent risk factor of PFS (HR=2.328, 95%CI= 1.042-5.203, P=0.039). Neither PFS nor OS are affected by the type of hysterectomy in patients without adjuvant therapy. In addition, the operation time of RH/mRH is longer than that of TH. Conclusion: RH/mRH will not improve PFS and OS in patients with clinical stageⅡEC, but will increase the length of surgery. In clinical treatment, there is no need for gynecologists to implement RH/mRH for patients with stageⅡEC.
Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice
Authors: Jiang Yingli , Peng Xiaojian , Zhang Qin , Liu Yuqing , Li Aiqi , Cheng Beijiu , Wu Jiandong 
Published: April 2022

Abstract
Abiotic stresses such as salinity and drought greatly impact the growth and production of crops worldwide. Here, a shikimate kinase-like 2 (SKL2) gene was cloned from rice and characterized for its regulatory function in salinity and drought tolerance. OsSKL2 was localized in the chloroplast, and its transcripts were significantly induced by drought and salinity stress as well as H2O2 and abscisic acid (ABA) treatment. Meanwhile, overexpression of OsSKL2 in rice increased tolerance to salinity, drought and oxidative stress by increasing antioxidant enzyme activity, and reducing levels of H2O2, malondialdehyde (MDA), and relative electrolyte leakage. In contrast, RNAi-induced suppression of OsSKL2 increased sensitivity to stress treatment. Interestingly, overexpression of OsSKL2 also increased sensitivity to exogenous ABA, with an increase in reactive oxygen species (ROS) accumulation. Moreover, OsSKL2 was found to physically interact with OsASR1, a well-known chaperone-like protein, which also exhibited positive roles in salt and drought tolerance. A reduction in ROS production was also observed in leaves of Nicotiana benthamiana showing transient co-expression of OsSKL2 with OsASR1. Taken together, these findings suggest that OsSKL2 together with OsASR1 act as important regulatory factors that confer salt and drought tolerance in rice via ROS scavenging.
SIRT2 overexpression alleviates remifentanil-induced postoperative hyperalgesia through microglia
Authors: Bo Jinhua , Zhu Wei , Wang Xinmei , Jiang Ming , Liang Ying , Ma Zhengliang 
Published: April 2022

Abstract
Background: Sirtuin 2 (SIRT2), a member of the mammalian sirtuin family, plays an important role in the pathogenesis of various neurological diseases. However, whether SIRT2 is involved in the regulation of remifentanil-induced postoperative hyperalgesia remains unclear. This study aimed to investigate the potential role of SIRT2 in regulating remifentanil-induced postoperative hyperalgesia.Methods: Remifentanil-induced postoperative hyperalgesia model was established in rats. SIRT2 was over-expressed by injecting recombinant adenoviruses. Nociceptive behaviors of mechanical allodynia and thermal hyperalgesia were measured with paw withdrawal mechanical threshold and paw withdrawal thermal latency, respectively. Iba1 (marker of microglia activation) and SIRT2 levels were detected with Western blot. Iba1 was also measured with immunofluorescence.Results: We found that SIRT2 was downregulated in the spinal cord in remifentanil-induced postoperative hyperalgesia rats. Intrathecal injection of a recombinant plasmid expressing SIRT2 markedly alleviated mechanical allodynia and thermal hyperalgesia in remifentanil-induced postoperative hyperalgesia rats. Iba1 were upregulated after surgical incision and remifentanil infusion, and the up-regulation was more obvious after the combination of surgical incision and remifentanil infusion. Moreover, our results showed that overexpression of SIRT2 inhibited the activation of the microglia in the spinal cord of remifentanil-induced postoperative hyperalgesia rats. Overexpression of SIRT2 significantly attenuated incision- and/or remifentanil induced pronociceptive effects and spinal microglia activation. Conclusions: Overexpression of SIRT2 alleviates remifentanil-induced postoperative hyperalgesia possibly via inhibition of spinal microglia activation. Therefore, SIRT2 may serve as a potential therapeutic target for treatment of neuropathic pain.Trial registration: Not applicable.