bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gram-Scale Synthesis of (R)-P-Chlorophenyl-1,2-Ethanediol at High Concentration by a Pair of Epoxide Hydrolases
Authors: Zhang Dong , Lei Yuqing , Wang Tingting , Lin Wenqian , Chen Xingyi , Wu Minchen 
Published: February 2022

(R)-p-chlorophenyl-1,2-ethanediol (pCPED) is an important intermediate for the synthesis of (R)-eliprodil that is widely applied in the treatment of ischemic stroke. To prepare (R)-pCPED with high enantiomeric excess (eep) and yield via the enantioconvergent hydrolysis of racemic styrene oxide (rac-pCSO) at high concentration, the bi-enzymatic catalysis was designed and investigated by a pair of epoxide hydrolases, a mutant (PvEH1Z4X4-59) of Phaseolus vulgaris EH1 and a mutant (RpEHF361V) of Rhodotorula paludigena RpEH. Firstly, the maximum allowable concentration of rac-pCSO was confirmed. Subsequently, the addition mode and the weight ratio of two Escherichia coli cells were optimized. Finally, under the optimized reaction conditions—the cell weight ratio 20:1 of E. coli/pveh1z4x4-59 to E. coli/rpehF361V, a simultaneous addition mode, and reaction temperature at 25°C—300 mM rac-pCSO in the 100 ml 4% (v/v) Tween-20/phosphate buffer system (100 mM, pH 7.0) was completely hydrolyzed within 5 h, affording (R)-pCPED with 87.8% eep, 93.4% yield, and 8.63 g/L/h space–time yield (STY). This work would be an efficient technical strategy for the preparation of chiral vicinal diols at industrial scale.
Mechanical Properties of Lightweight Foamed Concrete With Ceramic Tile Wastes as Partial Cement Replacement Material
Authors: Lim Siong Kang , Lee Yee Ling , Yew Ming Kun , Ng Wen Wei , Lee Foo Wei , Kwong Kok Zee , Lim Jee Hock 
Published: February 2022

The production of concrete emits greenhouse gases which aggravate global warming. Meanwhile, ceramic tile wastes generated from manufacturing factories, construction sites, and building demolition projects disposed of by landfills create land and water pollution. Therefore, in this study, the suitability of ceramic tile wastes (CTW) in powder form to partially replace cement in lightweight foamed concrete was investigated with the aim to discover a greener construction material. Once the research outputs show that the ceramic tile dust can appropriately serve as a replacement, environmental impact on air, land, and water pollution can all be reduced. Three types of lightweight foamed concrete with 0%, 25%, and 50% ceramic tile wastes as partial cement replacement material were prepared with a target density of 1,200 kg/m3, namely, LFC-CTW0, LFC-CTW25, and LFC-CTW50, respectively. The effects of ceramic tile wastes on the engineering properties of lightweight foamed concrete were investigated. Concrete specimens were water-cured and tested for various mechanical properties at the concrete ages of 7, 28, and 56 days. Results from the lab experiments showed that the incorporation of 25% ceramic tile wastes into lightweight foamed concrete was more feasible than that of 50%. LFC-CTW25, which performed better than LFC-CTW0 for the splitting tensile strength test at day 56. The results for the compressive strength test, modulus of elasticity, and compressive toughness test showed a minor reduction after the incorporation of ceramic tile wastes. Based on the results, it can be concluded that it is feasible to use ceramic tile wastes up to 25% as partial cement replacement material to produce foamed concrete.
The Signature of Moderate Perinatal Hypoxia on Cortical Organization and Behavior: Altered PNN-Parvalbumin Interneuron Connectivity of the Cingulate Circuitries
Authors: Trnski Sara , Nikolić Barbara , Ilic Katarina , Drlje Matea , Bobic-Rasonja Mihaela , Darmopil Sanja , Petanjek Zdravko , Hranilovic Dubravka , Jovanov-Milosevic Natasa 
Published: February 2022

This study was designed in a rat model to determine the hallmarks of possible permanent behavioral and structural brain alterations after a single moderate hypoxic insult. Eighty-two Wistar Han (RccHan: WIST) rats were randomly subjected to hypoxia (pO2 73 mmHg/2 h) or normoxia at the first postnatal day. The substantially increased blood lactate, a significantly decreased cytochrome-C-oxygenase expression in the brain, and depleted subventricular zone suggested a high vulnerability of subset of cell populations to oxidative stress and consequent tissue response even after a single, moderate, hypoxic event. The results of behavioral tests (open-field, hole-board, social-choice, and T-maze) applied at the 30–45th and 70–85th postnatal days revealed significant hyperactivity and a slower pace of learning in rats subjected to perinatal hypoxia. At 3.5 months after hypoxic insult, the histochemical examination demonstrated a significantly increased number of specific extracellular matrix—perineuronal nets and increased parvalbumin expression in a subpopulation of interneurons in the medial and retrosplenial cingulate cortex of these animals. Conclusively, moderate perinatal hypoxia in rats causes a long-lasting reorganization of the connectivity in the cingulate cortex and consequent alterations of related behavioral and cognitive abilities. This non-invasive hypoxia model in the rat successfully and complementarily models the moderate perinatal hypoxic injury in fetuses and prematurely born human babies and may enhance future research into new diagnostic and therapeutic strategies for perinatal medicine.
Behavior of Estonian Oil Shale in Acidic Oxidative Conditions
Authors: Niidu Allan , Grénman Henrik , Muldma Kati , Kaldas Kristiina , Mikli Valdek , Lopp Margus 
Published: February 2022

Oxidation has been a long sought-after alternative to classical thermal processing of oil shale, in order to obtain valuable raw materials for the chemical industry. A number of different methods have been applied, but thus far, one of the most effective ways to transform oil shale to value added products, such as aliphatic terminal dicarboxylic acids, is oxidation with nitric acid. In order to obtain insight into the reactivity of oil shale in nitric acid, a study focusing on the kinetics and behavior of oil shale particles during oxidative leaching was performed. To that end, the particle size distribution, surface area, and carbon content were measured during the leaching process in addition to the amount of total residual solids. Determining the carbon content of the solid residue was proposed as a simple measure of the reaction progress, based on the hypothesis that all carbon measured by elemental analysis correspond to organic carbon since inorganic carbon is present as carbonate in the starting material and would have dissolved under the acidic conditions. To our surprise, the solid residue had a significant amount of organic carbon in the form of calcium oxalate mineral. Thus, measuring carbon content in the solid residue could provide only an indirect measure of the overall oxidation degree provided that the amount of oxalates was known. In general, the results revealed that the total solid residue amounts to between 20% and 34% of the initial values after 24 h of the reaction, while the total carbon content ranges from 4% to 14% of the starting values. These results show that we were able to extract around 90% of the organic carbon present in the solid phase.
Minimizing Guidewire Unwilling Passage and Related Perforation During Transradial Procedures: Prevention Is Better Than Cure
Authors: Xu Lili , Cao Jiatian , Zhang Meng , Yang Hongbo , Huang Zheyong , Song Yanan , Li Chenguang , Dai Yuxiang , Yao Kang , Wang Xiangfei , Zhang Feng , Qian Juying , Ge Junbo 
Published: February 2022

BackgroundCurrent guidewires for transradial coronary angiography had defects of passage difficulty or branch injury. This study sought to investigate the safety and efficiency of a novel method of active knuckle-angle 0.035-inch hydrophilic guidewire in transradial coronary angiography.MethodsPatients undergoing a transradial coronary procedure in our team from August 2015 to June 2020 were retrospectively investigated. We compared the demographic and interventional characteristics of 1,457 patients receiving advancement of unmodified guidewires (Traditional group) and 1,322 patients receiving advancement of the knuckle guidewire (Knuckle group). Afterwards we included 239 patients and randomized them according to a random number table to either the unmodified or the knuckle guidewire to further confirm the efficiency and safety of knuckle guidewire advancement.ResultsIn the retrospective analysis, unwilling passage of guidewire into branches occurred more in the Traditional group than in the Knuckle group (9.5 vs. 0.08%, p &lt; 0.001). Two patients in the Traditional group experienced guidewire-associated perforation. One patient was treated with covered stent for internal mammarian artery perforation, while the other was managed with compression for brachial branch perforation. In the randomized controlled study, unwilling passage of guidewire also occurred more in the Traditional group (10.8 vs. 1%, p &lt; 0.001). Median duration of guidewire advancement from the sheath to aortic root significantly decreased from 33 seconds in the Traditional group to 21 seconds in the Knuckle group.ConclusionActive knuckle angle guidewire represented a novel method to prevent unwilling passage and associated perforation with efficiency improvement and a reduction in radiation exposure.
Association of Lean Body Mass and Fat Mass With 1-Year Mortality Among Patients With Heart Failure
Authors: Ge Yilan , Liu Jiamin , Zhang Lihua , Gao Yan , Wang Bin , Wang Xiuling , Li Jing , Zheng Xin 
Published: February 2022

BackgroundPrior studies have found an unexplained inverse or U-shaped relationship between body mass index (BMI) and mortality in heart failure (HF) patients. However, little is known about the independent effects of each body component, i.e., lean body mass (LBM) and fat mass (FM), on mortality.MethodsWe used data from the China Patient-centered Evaluative Assessment of Cardiac Events-Prospective Heart Failure Study. LBM and FM were calculated using equations developed from the National Health and Nutrition Examination Survey. LBM and FM index, calculated by dividing LBM or FM in kilograms by the square of height in meters, were used for analysis. We used restricted cubic spline and Cox model to examine the association of LBM and FM index with 1-year all-cause mortality.ResultsAmong 4,305 patients, median (interquartile range) age was 67 (57–76) years, 37.7% were women. During the 1-year follow-up, 691 (16.1%) patients died. After adjustments, LBM index was inversely associated with mortality in a linear way (P-overall association &lt; 0.01; P-non-linearity = 0.52), but no association between FM index and mortality was observed (P-overall association = 0.19). Compared with patients in the 1st quartile of the LBM index, those in the 2nd, 3rd, and 4th quartiles had lower risk of death, with hazard ratio of 0.80 (95% CI 0.66–0.97), 0.65 (95% CI 0.52–0.83), and 0.61 (95% CI 0.45–0.82), respectively. In contrast, this association was not observed between FM index quartiles and mortality.ConclusionHigher LBM, not FM, was associated with lower 1-year mortality among HF patients.
Amiodarone-Induced Multi-Systemic Toxicity Involving the Liver, Lungs, Thyroid, and Eyes: A Case Report
Authors: You Hye-Su , Yoon Jae Hyun , Cho Sung Bum , Choi Yoo-Duk , Kim Yung Hui , Choi Wonsuk , Kang Ho-Cheol , Choi Sung Kyu 
Published: February 2022

ObjectivesAmiodarone is widely used to treat arrhythmia. However, amiodarone is known for its severe toxicity to the liver, lungs, and thyroid. Amiodarone causes liver damage ranging from asymptomatic serum aminotransferase elevation to hepatic failure requiring liver transplantation. Although amiodarone toxicity has been reported, its simultaneous multi-organ toxicity is not well-known. Here, we introduce a novel case of multi-systemic amiodarone toxicity involving the liver, lungs, thyroid, and eyes.Case PresentationA 61-year-old woman visited the emergency room due to general weakness, nausea, visual disturbance, heat intolerance, and a non-productive cough. The patient had been using clopidogrel and amiodarone due to underlying atrial fibrillation. The total level of bilirubin was 0.71 mg/dL, aspartate aminotransferase was 358 U/L, alanine aminotransferase was 177 U/L, and prothrombin time was 27.1 s. Computed tomography showed diffuse increased liver intensity and scattered hyperattenuated nodular consolidations in both lungs. Transthoracic needle lung biopsy revealed fibrinoid interstitial inflammation with atypical change of type II pneumocytes and intra-alveolar foamy macrophages. In addition, the thyroid-stimulating hormone level was &lt;0.008 μIU/mL, and free thyroxine was 4.67 ng/dL. The thyroid scan showed diffuse homogenous intake of technetium-99 m pertechnetate in both thyroid lobes. The ophthalmologic exam detected bilateral symmetrical corneal deposits in a vortex pattern. With these findings, we could diagnose amiodarone-induced hepatic, pulmonary, thyroid, and ophthalmologic toxicity. Liver function was restored after cessation of amiodarone, and thyroid function was normalized with methimazole administration. However, due to aggravated lung consolidations, systemic steroid treatment was administered, and improvement was seen 1 week after, at the follow-up exam. As her symptoms improved, she was discharged with a plan of steroid administration for 3 to 6 months.ConclusionsThis case implies the possibility of multi-systemic amiodarone toxicity. Thus, the toxicity of amiodarone to multiple organs must be monitored. Prompt cessation of the drug should be considered upon diagnosis.
New Directions for Motivational Incentive Interventions for Smoking Cessation
Authors: Coughlin Lara N. , Bonar Erin E. , Walton Maureen A. , Fernandez Anne C. , Duguid Isabelle , Nahum-Shani Inbal 
Published: February 2022

BackgroundMotivational incentive interventions are highly effective for smoking cessation. Yet, these interventions are not widely available to people who want to quit smoking, in part, due to barriers such as administrative burden, concern about the use of extrinsic reinforcement (i.e., incentives) to improve cessation outcomes, suboptimal intervention engagement, individual burden, and up-front costs.PurposeTechnological advancements can mitigate some of these barriers. For example, mobile abstinence monitoring and digital, automated incentive delivery have the potential to lower the clinic burden associated with monitoring abstinence and administering incentives while also reducing the frequency of clinic visits. However, to fully realize the potential of digital technologies to deliver motivational incentives it is critical to develop strategies to mitigate longstanding concerns that reliance on extrinsic monetary reinforcement may hamper internal motivation for cessation, improve individual engagement with the intervention, and address scalability limitations due to the up-front cost of monetary incentives. Herein, we describe the state of digitally-delivered motivational incentives. We then build on existing principles for creating just-in-time adaptive interventions to highlight new directions in leveraging digital technology to improve the effectiveness and scalability of motivational incentive interventions.ConclusionsTechnological advancement in abstinence monitoring coupled with digital delivery of reinforcers has made the use of motivational incentives for smoking cessation increasingly feasible. We propose future directions for a new era of motivational incentive interventions that leverage technology to integrate monetary and non-monetary incentives in a way that addresses the changing needs of individuals as they unfold in real-time.
Direct Numerical Simulation of Heat Transfer of Lead–Bismuth Eutectic Flow Over a Circular Cylinder at Re = 500
Authors: Chen Li-Xia , Yuan Chao , Zhao Jun-Long , Zhang Hong-Na , Ma Yu , Li Feng-Chen 
Published: February 2022

The flow and heat transfer characteristics of the lead–bismuth eutectic (LBE) (Prandtl number Pr = 0.025) passing over a circular cylinder at Re = 500 are studied by direct numerical simulation and compared with the case of the air (Pr = 0.71). This article makes two major contributions: (1) heat transfer characteristics for the LBE flowing past a circular cylinder. For the case of air, the results show that a similarity exists among the wake structure of instantaneous temperature, fluctuating temperature, and velocity. However, these fields differ severely for the case of LBE. The local time-averaged Nusselt number (Nu¯) and circumferentially averagedNu¯are smaller in the LBE than that in the air. The circumferential and spanwise distributions ofNu¯in the LBE show a greater uniformity. The regions with larger circumferential or spanwise inhomogeneity appear in the flow separation zone. Besides, a resemblance between the distribution of the root mean square of fluctuation temperature and the turbulence kinetic energy can be recognized in the air; however, the similarity disappears for the case of LBE and in which the temperature fluctuation is smaller than that in the air. (2) Study on the temperature and velocity defect laws in the wake. By introducing the defect scales, it is concluded that the velocity field has not entered the self-preserving state yet, while the self-preserving state starts at the location of five times the diameter of the cylinder downstream of the cylinder for the temperature in the LBE and that of 21 times for the temperature in the air. In summary, even if without taking the buoyancy force into consideration, this article provides a fruitful description of the flow and heat transfer characteristics when the LBE flows past a cylinder, which is a typical flow in a helical coil steam generator of lead–bismuth alloy-cooled fast reactors. These highly resolved data on velocity and temperature are valuable for turbulence and heat fluxes modeling in the future and may facilitate the in-depth understanding of such flow and heat transfer characteristics within a limited variation range of operating temperature.
Genetics of Inherited Retinal Diseases in Understudied Populations
Authors: Kannabiran Chitra , Parameswarappa Deepika , Jalali Subhadra 
Published: February 2022

Retinitis pigmentosa is one of the major forms of inherited retinal dystrophy transmitted in all Mendelian and non-Mendelian forms of inheritance. It involves the loss of retinal photoreceptor cells with severe loss of vision or blindness within the first 2 decades of life. RP occurs at a relatively high prevalence in India and is often associated with consanguinity in certain South Asian communities where this practice is customary. This review describes the studies that have been published with regard to genetics of retinitis pigmentosa in India and neighboring South Asian countries. These populations have been understudied in these aspects although to a variable degree from one country to another. Genetic studies on RP in India have been carried out with a range of methods aimed at detecting specific mutations, to screening of candidate genes or selected genomic regions, homozygosity mapping to whole genome sequencing. These efforts have led to a molecular genetic characterization of RP in Indian families. Similar studies on large extended families from Pakistan have provided insight into several novel genes underlying the pathogenesis of these diseases. The extreme degree of clinical and genetic heterogeneity of RP renders it challenging to identify the associated genes in these populations, and to translate the research output towards better management of the disease, as there are no unifying genetic features that are characteristic of any population so far.
IL-17A Mediates Demyelination by Activating A1 Astrocytes via SOCS3 During Angiostrongylus cantonensis Infection
Authors: Zhou Zongpu , Lin Tuo , Liu Zhen , Ding Qian , Ma Zhixuan , Li Wanqi , Xie Fukang , Lan Yue , Feng Ying 
Published: February 2022

BackgroundDemyelinating disease of the central nervous system is one of the most common neurological diseases and effective treatment is still under in-depth research. Our previous study showed that Angiostrongylus cantonensis infection can induce demyelination injury in mouse brains and IL-17A expression was shown to be significantly increased during this process. Moreover, we found that IL-17A inhibition attenuated the demyelination caused by A. cantonensis infection. However, the underlying mechanisms have not yet been fully elucidated.MethodsIL-17A neutralizing antibodies were injected into A. cantonensis infected mice to decrease IL-17A levels. The activation of glial cells in the brain and the expression of cell markers were detected by a variety of methods, including real-time quantitative PCR, western blotting, and immunofluorescence staining. The relationship between IL-17A and astrocyte activation was further identified by in vitro experiments. The role of SOCS3 in the IL-17A stimulating process was determined using RNA-seq data collection of infected mice and the siRNA interference method.ResultsDemyelination of the corpus callosum was relieved after administration of IL-17A neutralizing antibody and this was accompanied by decreased activation of A1 type astrocytes around this region. The expression of SOCS3 was attenuated and activation of astrocytes by IL-17A was mediated by the IL-17RA/STAT3/SOCS3 pathway. IL-17A not only directly damaged oligodendrocytes but also indirectly damaged oligodendrocytes through A1 astrocyte mediation. Specific siRNA inhibition of IL-17A-inducible SOCS3 in astrocytes alleviated their damaging effects on oligodendrocytes.ConclusionIL-17A plays an important role in demyelination induced by A. cantonensis infection via the IL-17RA/STAT3/SOCS3 pathway in A1-type astrocytes, indicating that specific blockage of IL-17A and SOCS3 activity could be a therapeutic strategy for neuroinflammatory demyelinating diseases associated with astrocyte activation.
Effect of Extracted Titanium Tailing Slag on the Properties of Alkali-Activated Fly Ash-Ground Blast Furnace Slag Binder
Authors: Wang Shuping , Li Jingjing , Yun Xiaoxin , Lv Xuewei , Zhao Yujie , Zhang Zhigang 
Published: February 2022

The extracted titanium tailings slag (TS) is a by-product of titanium extraction from the blast furnace slag. Its chemical composition is similar to that of ground granulated blast furnace slag (GGBS), but the relatively lower reactivity limited its utilization as a supplementary cementitious material. In this study, the reactivity of TS was improved by mechanical grinding, and the optimum property of finely ground TS was selected to replace fly ash (FA) in the alkali-activated FA-GGBS binder. The influence of TS content on the fluidity, setting time, and compressive strength of the ternary binder was investigated. X-Ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy were used to analyze the effect of TS on the reaction process. Results showed that the optimum reactivity index of TS was 81.18% at 28 days when it was ground for 15 min in a ball mill. The highest compressive strength of the alkali-activated ternary binder was achieved when the replacement of FA by ground TS was 40%, and its 28-day compressive strength was 49.3 MPa, approximately 22% higher than the binder without TS. The addition of the TS would accelerate the reaction process of binder to form more products, including C-(A)-S-H and gismondine.