bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract 21 High-Throughput Blood Separation System for CD34+ Cell Isolation
Authors: Fedenko Shannon , McFetridge Calum , Milliron Hallie , Dobson Katherine 
Published: September 2022

Abstract

Introduction
Research into the therapeutic roles and clinical potential of CD34+ cells is growing [Weissman and Shizuru, Blood. 2008;112(9):3543-3553]. Accordingly, advances in cell isolation methods have risen to meet the research demand. Cord blood banks have a unique opportunity to source CD34+ cells because of their relatively high prevalence in cord blood [Dauber et al. Cytotherapy. 2011;13(4):449-458]. However, current isolation systems are not optimized for processing cord blood, and even blood-compatible systems typically require the blood to be processed before use. FerroBio (www.42bio.com) has developed a magnetic separation system that facilitates scalable, high-volume cell isolation from complex biological fluids, such as blood, without pre-processing. This technology is now being adapted for CD34+ cell isolation from cord blood.

Objective
In this study, the conditions in which magnetic beads efficiently bind target cells were explored. The objective of this work was to evaluate optimal blood mixing conditions to bring beads into contact with cells in suspension in the blood. This will allow unmodified cord blood units (CBUs) to flow directly into our apparatus.

Methods
The FerroBio separation process is comprised of three simple steps (Figure 1A). Here, conditions of step 1 were explored to maximize target cell recovery and viability. Variables included type of agitation equipment (Figure 1B), temperature, time, and relative air volume in the blood bag. Recovery and viability were measured via flow cytometry and calculated relative to unprocessed blood from the same CBU.

Results
Two mixing conditions were identified for maximum recovery of CD34+ cells (Figure 1C, blue bars). While not statistically different from each other, the Drum Roller method only requires one piece of standard equipment, is gentler, and can accommodate multiple CBUs simultaneously. No statistical difference in viability was observed in any conditions (90.7% ± 16.3%, n = 9). Increasing incubation time and the relative amount of air in the bag did not increase recovery (not shown).

Discussion
The FerroBio separator can streamline CD34+ cell isolation by processing high blood volumes in a fast, closed system with high recovery rates. Additional studies are under way to evaluate the functionality of the isolated CD34+ cells and efficiency of bead removal.
Abstract 25 ECTmatch: Optimizing Small-Scale Cord Blood Banking Through HLA Analysis
Authors: Dumont-Lagacé Maude , Feghaly Albert , Thauvette Gabrielle , Meunier Marie-Christine , Lemieux William , Fournier Diane , Richard Lucie , Lemieux Sébastien , Sauvageau Guy , Cohen Sandra 
Published: September 2022

Abstract

Introduction
Cord blood (CB) banks have had to rely on large inventories of CB units to try to serve the largest possible proportion of the population, all the while prioritizing collection of non-Caucasian ethnic groups. However, due to the high linkage disequilibrium of HLA genes and the high frequency of several HLA alleles in the population, CB banks contain hundreds of CB units that could be matched to the same patients, making the inventory somewhat redundant from a clinical standpoint.

Objective
ExCellThera developed ECTmatch, an algorithm dedicated to optimizing the selection of CB units based on in-depth HLA analysis in order to maximize the efficiency of the bank to suitably match the largest proportion of subjects within a small pool of donors.

Methods
The performance of ECTmatch was evaluated in a simulation aiming to select 100 CB units from the Héma-Québec CB bank that satisfied an arbitrary minimal cell content criteria of 120 × 107 TNC and 6 × 106 CD34+ cells (n = 2,987). Selection was performed to optimize matching for the Quebec population, with a minimal HLA-match of 5/8 for HLA-A, -B, -C, and -DRB1.

Results
ECTmatch provides a suitably matched donor for 71.5% of the Quebec population, compared with only 45.0% (±2.4%) with random selection. Because patients who require a CB transplant tend to have rarer HLAs, the performance of ECTmatch was evaluated for this specific subset of patients (n = 62). Again, ECTmatch outperformed random selection, by providing a donor for 54.8% of patients, compared with only 29.9% with random selection. Finally, while ECTmatch was developed to optimize CB selection specifically for the Quebec population, it still outperformed random selection for subjects from the other Canadian provinces or the USA.

Discussion
By selecting CB units based on HLA profiles, ECTmatch allows the creation of a highly useful inventory with a very low number of CB units. This approach to small-scale CB banking can be adapted to different population subsets and could be used to select a subset of CB units for pre-release for immediate clinical availability or for the creation of a pre-expanded CB inventory with maximal population coverage.
Abstract 26 HLA Analysis of the Australian Cord Blood Banks: How Diverse Are Donors?
Authors: Klamer Guy , Sue Jessica , Ko Kap-Hyoun , Trickett Annette , Johnson Phillip , Elwood Ngaire 
Published: September 2022

Abstract

Introduction
The network of public cord blood banks (CBB) in Australia, known as AusCord, is comprised of CBB located in Brisbane, Sydney, and Melbourne. The network stores almost 37,000 cord blood units (CBU) and has released more than 1,300 for transplantation.

Objective
The objectives of this study were (1) to determine the relative diversity of HLA allele subtypes, tissue types, and haplotypes at each of the banks and between the banks and (2) to identify common tissue types and haplotypes that could be utilized for clinical research and development of third party cell therapy products.

Methods
HLA data was obtained for 36,782 CBU stored in the AusCord inventory. To standardize data format, high-resolution typing was converted to 2-digit typing. HLA allele subtypes were ranked from most to least common. A subset of Indigenous Australian and Pacific Islander HLA allele subtypes was interrogated to determine whether increased frequency correlated with declared ethnicity. Tissue types were separated into CBU that had HLA-A, HLA-B, HLA-C, and HLA-DRB1 typing (21,815 total) and CBU that did not have HLA-C typing (36,782 total). CBU tissue types were interrogated in 3 separate groups: searchable, searched, and released inventory. Haplotypes were confirmed where maternal typing was available (3,105 CBU).

Results
Ethnicity screening for donors and strategic location of collection sites servicing ethnic minority communities resulted in banking of Indigenous Australian and Pacific Islander HLA. At a bank and network level, there was a similar frequency of specific HLA subtypes; however, when considering the tissue types, there was vast diversity (~75% unique with 8 allele typing). Whilst there is diversity, there was also a fraction of the inventory that exhibited repetitive tissue types that could be utilized for clinical research.

Discussion
The study demonstrates that cord blood collections are able to boost storage of diverse tissue types in, and unique to, a multicultural population such as Australia. This finding can guide policy development and funding, as well as operational decisions at the CBB. Furthermore, CBB are well positioned to support research and development activity aimed at discovery of new applications for cord blood units through supply of GMP grade cryopreserved products exhibiting common tissue types and haplotypes.
Abstract 28 Comparative Analysis of the Cord Blood Bank in Quebec
Authors: Lemieux William , Richard Lucie , Fournier Diane , Joron Susie , Sapir-Pichhadze Ruth , Lewin Antoine 
Published: September 2022

Abstract

Introduction
In the context of the regional diversity observed in the population of Quebec, the Héma-Québec Cord Blood Bank (HQ CBB) representation differs from that of the Héma-Québec Stem Cell Registry (HQ Registry). The Bank encourages registrants of non-European descent to promote diversity, and only collects from health centers around Montreal and Quebec cities because of proximity concerns.

Objective
In this study, we aimed to analyze allele frequencies in the HQ CBB to assess similarities and differences in reference to the HQ Registry.

Methods
The HQ Registry was previously typed using high-resolution genotyping. For the HQ CBB, ambiguous two-field HLA-A, -B, -C, and -DRB1 typing data were obtained from cord blood units. Statistical analyses were performed using the GENE[RATE] population analysis tools to estimate allele and haplotype frequencies by administrative region determined by registered postal codes. Hardy-Weinberg equilibrium was verified by the nested likelihood procedure. Finally, genetic distance was compared between the HQ Registry and the HQ CBB by region using Non-metric Multi-dimensional Scaling (NMDS).

Results
Because of the small sample size in certain regions, HLA frequency was analyzed for 7 of the 17 regions within the HQ CBB with typings from 11,472 cord blood units. The HLA allele frequencies were previously assessed for the Registry, with 3,806 donors originating from 14 administrative regions. The Hardy-Weinberg equilibrium was rejected at least in relation to one locus in 3 regions (Capitale-Nationale, Montreal, and Laval), which differs from the results obtained from the Registry, in which the equilibrium was rejected only for Montreal. As in the Registry, allele frequencies in the HQ CBB varied by region but had limited overlap with the HQ Registry. Two regions (Chaudière-Appalaches and Laval) demonstrated significant differences in allele frequencies as represented in the HQ Registry versus the HQ CBB.

Discussion
We observed differences in the Quebec population representation between the HQ CBB and the HQ Registry. This probably represents selection bias related to recruitment strategies and inclusion criteria. Importantly, the complementarity of these two stem cell sources could allow for a more diverse donor pool, and this understanding could also guide the recruitment strategies.
Abstract 30 Should Cord Blood Unit Distribution Patterns Impact Collection Strategies?
Authors: Frenet Emeline Masson , Sung Dorothy , Romeo Cynthia , Jimenez Alexandra , Weinberg Rona Singer 
Published: September 2022

Abstract

Introduction
The National Cord Blood Program has a large inventory of clinical cord blood units (CBUs) available for transplant (more than 60,000). As a small proportion is distributed each year, new collections should theoretically replenish the inventory, but it is unclear which characteristics should be prioritized for banking.

Objective
Our goal was to characterize distributed CBUs (in terms of cellularity, race/ethnicity, and match), compare them with the inventory, and devise a collection strategy.

Methods
Shipments from January 2015 to March 2022 were reviewed (n = 1,046). Race/ethnicity information on CBUs was obtained at the time of collection and was available for 55,934 stored CBUs and 1,020 distributed CBUs. It was also obtained for 394 recipients. HLA match was determined as HLA-A and B antigens and DRB1 allele match (/6), and allelic match as HLA-A, B, C, DRB1 allele match (/8). Total nucleated cells (TNC) and CD34 cells were counted at banking of the CBU, and cell doses (per kg of recipient’s weight) were calculated at shipment.

Results
The proportion of White Non-Hispanic (WNH) CBUs distributed (38%) was lower than what is present in the inventory (46%), but match levels were higher among these units (Figure 1A and 1B), with 55% of WNH CBUs being 5/8 or higher, vs 40% for minorities’ CBUs. Race match between CBU and recipient led to higher matched transplants, but 41% of race mismatched CBUs still reached 6/8 or higher allele match. The median TNC was 102 × 107 for stored CBUs and 167 × 107 for distributed CBUs (Figure 1C). Better matched units have been selected with lower cell contents (Figure 1D).

Discussion
CBUs broaden transplant options across ethnic groups, and much focus has been placed on collecting minorities’ CBUs. However, our analysis showed that WNH CBUs are still the largest distributed population, with higher allele match to the recipient (predicting better transplant outcomes), so these CBUs should not be excluded from collection strategies. From the data presented here, the goal for new collections should be to obtain CBUs with higher TNC and CD34 (spreading across all races and ethnicities). These would replenish the pool of high cellularity CBUs that are more often selected for transplant.
Abstract 32 Low Incidence of Product Complaints and Adverse Events Support Safety of Cord Blood Units
Authors: O’Gara Erin , Regan Donna , Rice Lindsay , Bakken Ruth 
Published: September 2022

Abstract

Introduction
As an important cellular therapy source for many patients, the safe and timely delivery of cord blood units (CBU) is paramount. The National Marrow Donor Program (NMDP) investigates product complaints received for CBUs distributed under the 10-CBA investigational new drug application that may impact the safety, quality, identity, purity, or potency of the product. NMDP also receives reports of any patient adverse events or serious complications resulting from the transplantation of CBUs. Information on product complaints and adverse events provides insight into areas related to product integrity, patient safety, and customer satisfaction and highlights opportunities for improvement.

Objective
We analyzed all product complaints and adverse events reports submitted to NMDP for CBUs distributed under the NMDP 2010 Cord Blood Access Protocol (10-CBA) from October 2019 through March 2022. These investigations offer visibility into factors associated with product quality and integrity discovered after shipment.

Methods
A total of 1,578 CBUs from 38 cord blood banks were shipped from October 2019 through March 2022 (30 months). Data on CBU product complaints reported to NMDP were compiled and analyzed. Data were grouped into categories based upon the primary reason for the product complaint or adverse event. The outcome of NMDP investigations and resolutions is relayed quarterly to the Quality Subgroup of the Cord Blood Advisory Group.

Results
Of the 1,578 CBUs shipped between October 2019 and March 2022, 98% had no reported incidents (32 reports of product complaints and 4 of adverse events). The most common reasons for product complaints investigated by NMDP were related to shipping/transportation (n = 15, 47%), quality issues such as lower than expected potency or bag breaks (n = 11, 34%), and labeling errors (n = 3, 9%). Three of the four adverse events were associated with infusion (two hypertensive episodes and one anaphylaxis) and one was a secondary cancer following transplant.

Discussion
Transplant centers report very few quality incidents, product complaints, or adverse events when using cord blood units. We remain vigilant for opportunities to improve. Clinicians and patients can be assured that CBUs are a safe product with high likelihood of arriving to the transplant center without incident or causing adverse events once transplanted.
Rare Concurrence of Alopecia Areata in the Setting of the Lipedematous Scalp
Authors: Hu Danchen , Yang Shuxia 
Published: September 2022

Abstract:
The lipedematous scalp (LS) is characterized by a thickened scalp because of the increased thickness of the subcutaneous fat layer. When the soft and boggy scalp is associated with shortened hairs and hair loss, it is referred to as lipedematous alopecia (LA). We report a case of alopecia areata with LS, which may be misdiagnosed as LA. However, the histopathologic features showed a thickened subcutaneous fat layer and hair bulb inflammation. Hair regrowth was appreciable after treatment with topical steroids, minoxidil, oral compound glycyrrhizin, and vitamin D. This case report aims to show that LS and alopecia areata may coexist, and histopathologic examination is necessary for precise diagnosis.
Climacteric status is associated with sexual dysfunction at the age of 46 years: a population-based study
Authors: Savukoski Susanna M. , Pinola Pekka A. , Pesonen Paula R. O. , Puukka Katri S. , Suvanto Eila T. J. , Niinimäki Maarit J. 
Published: September 2022

Abstract

Objective
Increasing age and menopausal transition increase the risk of sexual dysfunction. Sexual dysfunction is common in women experiencing menopause before the age of 40 years, whereas evidence on sexual function in women experiencing menopause in their mid-40s is scarce. We aimed to investigate sexual function in 46-year-old women in relation to their menopausal status.

Methods
This study cross-sectionally evaluated sexual function of women in a prospective population-based Northern Finland Birth Cohort 1966 (NFBC1966). A 46-year follow-up study of NFBC1966 included a broad questionnaire evaluating health, lifestyle, and life situation, as well as menstrual history and sexual function, and blood sampling analysis including follicle stimulating hormone and free androgen index (FAI). The participants were divided into two groups by their menopause status, defined by follicle-stimulating hormone and menstrual history. We performed logistic regression models in which parameters of sexual function were dependent factors and climacteric status, self-reported health, FAI, relationship status, smoking, and education level were independent variables.

Results
The study population included 2,661 women. In regression models, more advanced climacteric status was associated with higher frequency and difficulty level of low sexual desire and vaginal dryness (odds ratios with 95% confidence intervals: 2.80 [2.12-3.71], 3.22 [2.43-4.27], 3.83 [2.82-5.20], 3.75 [2.75-5.12], respectively), lower frequency of sexual thoughts (1.34 [1.02-1.75]), and higher frequency of problems with intercourse (2.35 [1.51-3.66]). Lower FAI and poorer health were associated with impaired sexual function.

Conclusions
The current study suggests that women experiencing menopausal transition in their mid-40s are at risk of impaired sexual function.
CHANGE IN CHORIOCAPILLARIS FLOW DEFICITS WITHIN TEARS OF THE RETINAL PIGMENT EPITHELIUM IMAGED WITH SWEPT-SOURCE OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY
Authors: Iyer Prashanth G. , Chu Zhongdi , Shen Mengxi , Shi Yingying , Trivizki Omer , Zhang Qinqin , Feuer William , Wang Ruikang K. , Gregori Giovanni , Rosenfeld Philip J. 
Published: September 2022

Purpose:
Choriocapillaris (CC) flow deficits (FDs) were measured in the areas exposed by tears of the retinal pigment epithelium (RPE) before and after their onset to determine their change over time.

Methods:
Patients enrolled in a prospective, swept-source optical coherence tomography angiography (SS-OCTA) study were retrospectively reviewed for RPE tears, and scans were evaluated before and after RPE tear formation. Choriocapillaris flow deficits were measured within the bed of the tear and within a symmetric control region.

Results:
Three patients with RPE tears were imaged before tear formation and for at least 16 months afterward. When the baseline and first posttear visit were compared, CC FDs decreased by 1.0% in the tear region and 1.7% in the control region (P = 0.84). When the 16-month follow-up visits were compared with the first post-RPE tear visits, CC FDs decreased by 1.9% in tear regions and increased by 1.3% in control regions (P = 0.37).

Conclusion:
No significant changes in CC FDs were observed before and after RPE tear formation and for 16 months afterward, suggesting that CC FDs can be reliably detected in the presence of an intact RPE and the absence of the RPE did not affect CC perfusion for at least 16 months.
Decreases in Nephrotoxic Pain Medications Are Not Associated With Increased Opioid Utilization in Critically Ill Children
Authors: Stenson Erin K. , DeMasellis Gina , Shah Ananya , Reiter Pamela D. , Brinton John , Martin Sara , Iwanowski Melissa , Rannie Michael , Soranno Danielle E. , Gist Katja M. 
Published: September 2022

OBJECTIVES:
Quality improvement initiatives to decrease rates of nephrotoxic medication exposure have reduced rates of acute kidney injury (AKI) in noncritically ill children. The objective of our study was to analyze the implementation of a similar program in critically ill children and to measure important balancing measures including opioid and benzodiazepine exposure.

DESIGN:
Prospective quality improvement study.

SETTING:
PICU at Children’s Hospital Colorado between 2018 and 2020.

PATIENTS:
All children admitted to PICU.

INTERVENTIONS:
Quality improvement initiative called Nephrotoxic Injury Negated by Just-In-Time Action (NINJA).

MEASUREMENT AND MAIN RESULTS:
Eight thousand eight hundred thirty-three PICU patient admissions were included. Mean rates of nephrotoxic medication exposure/1,000 PICU patient days decreased from 46 to 26, whereas rates of nephrotoxic AKI/1,000 PICU patient days did not change. Nonsteroidal anti-inflammatory drug dispenses per 1,000 patient days were reduced from 521 to 456. Similarly, opioid and benzodiazepine exposures per 1,000 patient days were reduced from 812 to 524 and 441 to 227, respectively, during the study observation period.

CONCLUSIONS:
The NINJA intervention was efficaciously implemented in our single-center PICU. Nephrotoxic exposure is a modifiable factor that did not inadvertently increase exposure to opioids and benzodiazepines.