bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coculture of ARPE-19 Cells and Porcine Neural Retina as an Ex Vivo Retinal Model
Authors: Wagner Natalie , Safaei Armin , Vogt Pia A. , Gammel Maurice R. , Dick H. Burkhard , Schnichels Sven , Joachim Stephanie C. 
Published: March 2022

Neural retinal organ cultures are used to investigate ocular pathomechanisms. However, these cultures lack the essential retinal pigment epithelium (RPE) cells, which are part of the actual in vivo retina. To simulate a more realistic ex vivo model, porcine neural retina explants were cocultured with ARPE-19 cells (ARPE-19 group), which are derived from human RPE. To identify whether the entire cells or just the cell factors are necessary, in a second experimental group, porcine neural retina explants were cultured with medium derived from ARPE-19 cells (medium group). Individually cultured neural retina explants served as controls (control group). After 8 days, all neural retinas were analysed to evaluate retinal thickness, photoreceptors, microglia, complement factors and synapses ( n = 6–8 per group). The neural retina thickness in the ARPE-19 group was significantly better preserved than in the control group ( p = 0.031). Also, the number of L-cones was higher in the ARPE-19 group, as compared to the control group ( p < 0.001). Furthermore, the ARPE-19 group displayed an increased presynaptic glutamate uptake (determined via vGluT1 labelling) and enhanced post-synaptic density (determined via PSD-95 labelling). Combined Iba1 and iNOS detection revealed only minor effects of ARPE-19 cells on microglial activity, with a slight downregulation of total microglia activity apparent in the medium group. Likewise, only minor beneficial effects on photoreceptors and synaptic structure were found in the medium group. This novel system offers the opportunity to investigate interactions between the neural retina and RPE cells, and suggests that the inclusion of a RPE feeder layer has beneficial effects on the ex vivo maintenance of neural retina. By modifying the culture conditions, this coculture model allows a better understanding of photoreceptor death and photoreceptor–RPE cell interactions in retinal diseases.
Effectiveness of micro-lecture based cardiac rehabilitation education on health status in individuals with coronary artery disease: A randomized clinical trial
Authors: Li Ji-Na , Zeng Chao , Zhu Song , Mao Lian , Huang Ling-zhi 
Published: March 2022

Objective To explore the effectiveness of the micro-lecture education on the ability of self-management and lifestyle changes in patients with coronary artery disease. Design A randomized control trial with a 12-week follow-up. Setting Cardiology wards of a large Chinese University Hospital. Subjects One hundred eligible inpatients with coronary artery disease were recruited. Intervention Intervention group received the micro-lecture education, while the control group received the traditional cardiac rehabilitation education. Main measures Primary outcome of the ability of coronary self-management was assessed by the Coronary self-management scaleat baseline, 12-week after discharge. Secondary outcomes of smoking status, exercise status, and six-minute walk distance were evaluated at the baseline, week4 and week 12 after discharge. The chi-square test,Fisher's exact test, group t-test and the repeated measure variance were used for the data analysis. Results Eighty-nine of 100 eligible participants responded, and mean age was 60.2 ± 11.3 years.After the intervention, the mean score of the coronary self-management scale (3.84 ± 0.36) was higher than that in the control group (2.78 ± 0.57).At week 12, the rate of re-smoked in the intervention group (6%) was significantly lower than that in the control group (43%),the frequency of the exercise (85%), and the 6MWD (495.55 ± 85.94) in the intervention group were better than the control group. Conclusion Micro-lecture education program presented a positive effect on promoting the ability of self-management, and apotential effect on the therapeutic lifestyle changes for the coronary artery disease patients.
NF-kB pathway is involved in microscopic colitis pathogenesis
Authors: Pisani Laura Francesca , Tontini Gianeugenio , Vecchi Maurizio , Croci Giorgio Alberto , Pastorelli Luca 
Published: March 2022

Objective To investigate the potential inflammatory pathways involved in the development of microscopic colitis (MC). Methods This prospective study analysed human intestinal tissue that was collected and classified as healthy controls (HC), microscopic colitis (MC) and ulcerative colitis (UC). An RT2 Profiler PCR Array for human inflammatory response and autoimmunity was used to evaluate the expression of 84 specific genes related to the inflammatory and autoimmunity pathways. Data were validated by means of real-time polymerase chain reaction on an independent group of MC intestinal tissue samples. Results This study measured the expression of inflammatory genes in HC ( n = 10), in patients with MC ( n = 8) and in patients with active UC ( n = 10). Of the 84 genes included in the array, the expression of the C-C motif chemokine ligand 19, C-C motif chemokine ligand 21, lymphotoxin beta and complement C3 genes that are involved in the non-canonical nuclear transcription factor kappa B (NF-kB) pathway was increased by 2.96, 6.05, 5.96 and 5.93 times in MC compared with HC, respectively. These results were confirmed by real-time polymerase chain reaction. Conclusions The findings suggest that an impairment of the non-canonical NF-kB pathway is involved in the development of MC.
Diagnostic utility of T2*-weighted GRE in migraine with aura attack. The cortical veins sign
Authors: Hirtz Chloe , Adam Gilles , Raposo Nicolas , Fabre Nelly , Ducros Anne , Calviere Lionel , Rousseau Vanessa , Albucher Jean François , Olivot Jean-Marc , Bonneville Fabrice , Viguier Alain 
Published: March 2022

Objective To evaluate the frequency, distribution, and clinical associations of the dilated appearance of cerebral cortical veins, termed cortical veins sign on T2*-weighted gradient recalled-echo (T2*-GRE) in the acute setting of migraine with aura attack in adult patients. Methods We conducted a retrospective analysis of 60 consecutive patients admitted for acute neurological symptoms with a final diagnosis of migraine with aura (42%) or probable migraine with aura (58%) who underwent emergency brain magnetic resonance imaging and 60 non-migrainous control adults. The cortical veins sign was defined as a marked hypo-intensity and/or an apparent increased diameter of at least one cortical vein. We examined the prevalence, the spatial distribution, and the associations of cortical veins sign with clinical characteristics of migraine with aura. Results We detected the cortical veins sign in 25 patients (42%) with migraine with aura, compared to none in the control group ( p < 0.0001). The spatial distribution of cortical veins sign was characterised by the predominantly bilateral and posterior location. Presence of cortical veins sign was associated with increased severity of aura ( p = 0.05), and shorter delay to MRI ( p = 0.02). Conclusion In the setting of acute neurological symptoms, the presence of cortical veins sign is frequent in patients with migraine with aura and can be detected with good reliability. This imaging marker may help clinicians identify underlying migraine with aura.
Mutation screening and association analysis of NOTCH3 p.R544C in patients with migraine with or without aura
Authors: Wang Yen-Feng , Liao Yi-Chu , Tzeng Yi-Shiang , Chen Shih-Pin , Lirng Jiing-Feng , Fuh Jong-Ling , Chen Wei-Ta , Lai Kuan-Lin , Lee Yi-Chung , Wang Shuu-Jiun 
Published: March 2022

Background The role of the NOTCH3 p.R544C variant, the predominant variant of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy in multiple East Asian regions, in migraine is unknown. Methods Migraine patients (n = 2,884) (2,279F/605M, mean age 38.8 ± 11.7 years), including 324 (11.2%) with migraine with aura, were prospectively enrolled by headache specialists according to the International Classification of Headache Disorders criteria. These patients and 3,502 population controls free of stroke, dementia, and headache were genotyped for NOTCH3 p.R544C by TaqMan genotyping assay or Axiom Genome-Wide TWB 2.0 Array. Clinical manifestations and brain magnetic resonance images were examined and compared between migraine patients with and without NOTCH3 p.R544C. Results Thirty-two migraine patients (1.1%) and 36 controls (1.0%) harbored the p.R544C variant, and the percentages were comparable among migraine patients without and with aura, and controls (1.2%, vs. 0.6% vs. 1.0%, p = 0.625). Overall, migraine patients with and without the p.R544C variant had similar percentages of migraine with aura, headache characteristics, frequencies and disabilities. However, those with p.R544C were less likely to have pulsatile headaches (50.0% vs. 68.2%, p = 0.028), and more likely to have moderate to severe white matter hyperintensities in the external capsule (18.8% vs. 1.2%, p = 0.006) and anterior temporal lobe (12.5% vs. 0%, p = 0.008). Conclusions Our findings suggest that NOTCH3 p.R544C does not increase the risk of migraine with aura, or migraine as a whole, and generally does not alter clinical manifestations of migraine. The role of NOTCH3 variants, as well as potential influences from ethnicity or modifier genes, in migraine needs to be further clarified.
Ceftaroline: Systematic Review of Clinical Uses and Emerging Drug Resistance
Authors: Abate Getahun , Wang Grace , Frisby Jared 
Published: March 2022

Objective To assess the success rates of off-label uses of ceftaroline for infections caused by methicillin-resistant Staphylococcus aureus (MRSA) and evaluate emerging ceftaroline resistance. Data Sources We queried PubMed/MEDLINE, with the search term “Ceftaroline.” Articles were restricted to the English language and year of publication (January 1, 2009-January 31, 2022). Study Selection and Data Extraction Clinical trials, observational studies, and case reports that reported efficacy, safety, pharmacokinetics, use in MRSA infections other than acute bacterial skin infection and community-acquired pneumonia, and ceftaroline resistance were selected. Data Synthesis The search pooled 103 publications and all abstracts were reviewed. Forty-six articles that reported efficacy, safety, pharmacokinetics, or off-label use in multiple patients and 7 articles on ceftaroline resistance are used in this review. Ceftaroline has been approved for treatment of acute skin/soft tissue infection and community-acquired pneumonia. Ceftaroline’s efficacy in off-label infections ranged from 66.7% to 87.3% depending on the types of infection. There were 14 documented cases of ceftaroline resistance associated with PBP2a changes. Relevance to Patient Care and Clinical practice Case series and observational studies have documented success with ceftaroline alone or in combination with vancomycin or daptomycin for treatment of MRSA bone and joint, endovascular, diabetic foot infections, and bacteremia from other causes. Conclusion Despite the lack of randomized controlled trials, ceftaroline is used as salvage therapy for different MRSA infections. The data from case series and observational studies are promising but ceftaroline should be used judiciously as ceftaroline-resistant MRSA begin to emerge.
Stop posterior wall puncture of the arteriovenous graft (AVG). New findings of cannulation techniques from a prospective observational study with an AVG model and plastic cannula for dialysis
Authors: Kimura Go , Matsuyama Takuya , Mizuno Yuki 
Published: March 2022

Background: Posterior wall puncture of the AVG causes serious vascular access complications, but there is no concrete technical recommendation for AVG cannulation with plastic cannula. The purpose of this study is to identify cannulation techniques to reduce posterior wall puncture of the AVG using plastic cannula. Methods: Sixty-three hemodialysis nurses’ cannulations on experimental models were recorded and included in this study. Cannulations were conducted on AVG and AVF models with a plastic cannulation needle. We analyzed the angle of the needle, the motion of the needle, and the location of the needle in the graft lumen. Results: The occurrence of posterior wall puncture of the AVG model was 22.2%. The cannulation angles in the AVG model were greater than those in the AVF model ( p < 0.05). In the posterior wall puncture group of the AVG model, after the tip of the needle had reached into the graft lumen, the angle of the needle was not flattened ( p < 0.05) and the outer sleeve of the needle was not inserted into the graft ( p < 0.05). Furthermore, posterior wall puncture of the AVG model were observed in the group with less than 5 years of dialysis nursing experience ( p < 0.05). Conclusions: From this study, after the tip of the needle had reached into the graft lumen, flattening the angle of the needle and inserting the outer sleeve of the needle into the graft were suggested as specific cannulation techniques to reduce posterior wall puncture of the AVG. Furthermore, this study also suggests the importance of cannulation technique education for new dialysis nurses to reduce cannulation-caused complications.
Individual variations in British humour appreciation among Chinese–English bilinguals: Role of socialisation and acculturation
Authors: Chen Xuemei , Zhang Tiefu 
Published: March 2022

Aims and objectives/purpose/research questions: The primary aim of this study is to investigate individual differences in the appreciation of humour in an L2 with a special focus on British humour. It examined the predictive effects of variables including sociobiographical factors, linguistic profiles, domain-specific communication, and degree of acculturation on L2 (British) humour appreciation among late Chinese–English bilinguals. Design/methodology/approach: This study adopted a quantitative approach and used a video-embedded online questionnaire for data collection. A total of 131 UK-based late Chinese–English bilinguals rated the funniness of two short extracts from British sitcoms and answered questions regarding sociobiographical, linguistic, domain-specific communication, and cultural factors. Data and analysis: Funniness rating scores were calculated for each participant and for each humour extract. Spearman’s rho correlation analysis was used to find out the correlations between funniness ratings and different variables. Other non-parametric statistical tests such as Kruskal–Wallis test, Mann–Whitney U test, and Wilcoxon signed-ranks test were run to compare the differences between variables. Findings/conclusions: Chinese–English bilinguals who were more frequently engaged in humour-specific communication with friends and L1 speakers of British English and acculturated to a higher level tended to perceive L2 (British) humour funnier. Originality: The study highlights the role of humour socialisation and acculturation, along with L2 proficiency, in the appreciation of L2 humour. Sociobiographical factors associated with the appreciation of L1 humour may not necessarily predict the appreciation of humour in an L2. Significance/implications: The study contributes to the literature on the predictive factors for the domain of humour in an L2 among bilinguals and offers implications for both the field of bilingualism and humour.
Clinical communication in orthodontics: Any questions?
Authors: Stonehouse-Smith Daniel , Pandis Nikolaos , Bister Dirk , Seehra Jadbinder 
Published: March 2022

Objective: To measure patient-perceived standards of clinician communication and identify elements of deficient performance. Good communication can improve the quality of care, patient satisfaction and compliance with treatment. Design: Cross-sectional questionnaire service evaluation. Setting: Two university dental hospital orthodontic departments. Participants: Any patients aged 10 years and over attending the orthodontic department for treatment or consultation were eligible for inclusion. Patients who required third-party translation services were excluded. Methods: Clinicians provided the modified 15-item Communication Assessment Tool (CAT) to up to five patients in a clinical session. A front sheet for clinician characteristics was used and anonymised with a unique identifier. Univariable logistic GEE models examined associations among responses and clinician characteristics. Results: There were 55 clinicians with 204 patient responses. The overall percentage of ‘5=excellent’ ratings was 88% (SD 0.16). The lowest scoring item was ‘encouraged me to ask questions’ (55.8%). Based on clinician characteristics, there were lower odds of an excellent response for certain CAT items. There were higher odds of an excellent response if English was not the clinician’s first language (1.05; 95% confidence interval = 1.00–1.09; P=0.03). Conclusion: There is a high standard of patient–clinician communication in the hospital orthodontic setting. Key areas of communication that require attention include encouraging patients to ask questions, talking in terms they can understand, recognising their main concerns and involving them in the decision-making process. The results of this study can be used to inform communication skills training and be replicated in similar dental settings (primary and secondary care) as part of quality improvement.
Sport Participation and Subjective Outcomes of Health in Middle-Aged Men: A Scoping Review
Authors: Blake Henry T. , Buckley Jonathan D. , Stenner Brad J. , O’Connor Edward J. , Burgess Shane A. , Crozier Alyson J. 
Published: March 2022

Although sport participation is intrinsically motivating and improves the physical health of middle-aged men, its influence on subjective health measures, such as health-related quality of life, self-rated health, or well-being is unclear. The purpose of this scoping review was to describe the existing literature that has assessed male sport participants and their subjective health. MEDLINE, Embase, Emcare, PsycInfo, SPORTDiscus, Cochrane Library, and Web of Science were searched, and reference lists of included studies were pearled. Included were original peer-reviewed studies reporting a marker of subjective health in males, 35 to 54 years (average), who participated in sport. The search identified 21 eligible articles, 18 quantitative, 2 mixed-methods, and 1 qualitative, from 13 different countries. Eighteen studies were cross-sectional. A broad range of outcomes were assessed, with the most common being quality of life/health-related quality of life ( n = 6) and self-rated health ( n = 6). Most studies assessing quality of life, health-related quality of life, or self-rated health demonstrated a positive association with sport participation, while sport participation was not related to measures of life satisfaction, flourishing, happiness or global well-being; however, limited studies examined these latter outcomes. Sport participation appears to be related to better select subjective health outcomes in middle-aged men. However, most available data are cross-sectional and thus causation cannot be determined. Randomized intervention trials are required to determine whether sport participation improves the subjective health of middle-aged men. Open Science Framework registration: https://osf.io/zypds
Systematic Mapping Review of Orthognathic Surgery (Protocol)
Authors: Bendersky Josefina , Uribe Macarena , Bravo Maximiliano , Vargas Juan Pablo , Villanueva Julio , Urrutia Gerard , Bonfill Xavier 
Published: March 2022

Study Design This document details the planning phase of a systematic mapping review. Objective The objective of this mapping review is to identify, describe, and organize evidence currently available from systematic reviews and primary studies regarding different co-interventions and surgical modalities used in orthognathic surgery (OS) and their outcomes. Methods Systematic reviews (SRs), randomized controlled trials (RCTs), and observational studies that evaluate perioperative OS co-interventions and surgical modalities will be identified in an exhaustive search of MEDLINE, EMBASE, Epistemonikos, Lilacs, Web of Science, and CENTRAL. Grey literature will also be screened. Results Expected results include identification of all PICO questions available in the evidence regarding OS and generation of evidence bubble maps, involving a matrix of all identified co-interventions, surgical modalities, and outcomes presented in the studies. This will achieve identification of research gaps and prioritization of new research questions. Conclusions The significance of this review will result in a systematic identification and characterization of the available evidence, leading to a reduction in research waste and a guidance of future efforts in developing studies for unsolved questions.
Musculoskeletal disorders among the population in Northwest Ethiopia
Authors: Merkeb Alamneh Yoseph , Sume Bickes Wube , Abebaw Shiferaw Abtie 
Published: March 2022

Objective: This study aimed to assess the magnitude and patterns of chronic musculoskeletal disorders among the population in East Gojjam zone, Northwest Ethiopia. Methods: A community-based cross-sectional study with 846 recruited study participants was done using a multistage sampling technique in conjunction with a simple and systematic random sampling technique. Face-to-face interviews, physical examinations, a semi-structured questionnaire adapted from the Community Oriented Program for Control of Rheumatic Diseases, and other literature were used to collect data. EpiData 3.1 was used to enter the data, which were then exported to STATA 14.0 for analysis. Result: The study had a response rate of 98.8%, with about 836 people out of a total of 846 expected to take part. The magnitude of musculoskeletal disorders was found to be 40.1%. Back pain was the most common musculoskeletal condition (16%) of the population, followed by osteoarthritis (10%) and other forms of arthritis (rheumatoid arthritis (8%), gout arthritis (6%)). The most common comorbidity (multimorbidity) patterns were hypertension (9.8%), diabetes (5.6%), obesity (3.9%), and others (2.2%). Conclusion: Almost one in four participants have at least one musculoskeletal disorder in Northwest Ethiopia. Rheumatoid arthritis, osteoarthritis, low back pain, and gout arthritis were the most common musculoskeletal disorders. The most common comorbidity patterns were hypertension, diabetes, obesity, and others. An in-depth investigation of the musculoskeletal burden at the national level will be critical for implementing evidence-based strategies, as well as early detection and screening, linking to health institutions, and direct interventions.