bluuebunny/crossref_metadata_2025_split huggingface dataset:
High total water loss driven by low-fat diet in desert-adapted mice
Authors: Blumstein Danielle M. , Colella Jocelyn P. , Linder Ernst , MacManes Matthew D. 
Published: April 2022

AbstractAvailability of food resources is an important driver of survival. Animals must either relocate or adapt to persist in environments where critical resource abundance is changing. An optimal diet balances energy gain, water regulation, and nutrition. We used flow-through respirometry to characterize metabolic phenotypes of the desert-adapted cactus mouse (Peromyscus eremicus) under diurnally variable environmental conditions that mimic the environment of the Sonoran Desert. We treated mice with two different energetically equivalent diets, a standard diet and a low-fat diet, and measured energy expenditure, water loss rate, respiratory quotient, weight, and electrolyte levels. Mice fed the low-fat diet lost significantly more water than those on the standard diet. Our results suggest that cactus mice may have limited capacity to tolerate water deprivation if optimal foods become less abundant. Given that climate change is predicted to modify the distribution of food items, understanding these links may have important implications for long-term population viability for desert and non-desert adapted animals alike.
Study on the interference of DicdsVg4 and DicdsVgR in egg formation in the ovary of Diaphorina citri (Kuwayama)
Authors: Li Hailin , Wang Xiaoyun , Pan Biqiong , Zheng Xialin , Lu Wen 
Published: April 2022

ABSTRACTThe ovary is the key organ in insect female oviposition. For the continuation of populations in nature, the whole life cycle of female adults is centred on reproduction. Vg and VgR genes are important in determining the synthesis and transport of vitellogenin. Therefore, Vg and VgR gene expression in insect females affects the formation of mature eggs in the ovary, which is very important. RNAi interference of Vg and VgR genes may become an important means to control D. citri populations. A study found that the expression of the DicVg4 and DicVgR genes was significantly affected by DicdsVg4 and DicdsVgR. DicVg4 and DicVgR gene expression interference could prevent the accumulation of vitellogenin in the ovaries of females, rendering ovaries unable to form mature eggs normally and leading to the production of abnormal eggs and nymphs. After knockdown of DicVg4 and DicVgR gene expression, the proportion of ovarian mature eggs was only 8.33 ± 1.67% and 7.75 ± 0.5% in the 10-d developmental stage, the proportion of negative control group ovarian mature eggs was 13.19 ± 0.14% in the 10-d developmental stage, and the proportion of ovarian mature eggs in the interference group was 0.00 ± 0.00% in the 5, 15, 20, 25, and 30-d developmental stages. These results provide a theoretical basis for the use of mixed polygene interference technology to control damage from D. citri populations.
The impact of alternative delivery strategies for novel tuberculosis vaccines in low- and middle-income countries: a modelling study
Authors: Clark Rebecca A. , Mukandavire Christinah , Portnoy Allison , Weerasuriya Chathika K. , Deol Arminder , Scarponi Danny , Iskauskas Andrew , Bakker Roel , Quaife Matthew , Malhotra Shelly , Gebreselassie Nebiat , Zignol Matteo , Hutubessy Raymond C.W. , Giersing Birgitte , Jit Mark , Harris Rebecca C. , Menzies Nicolas A. , White Richard G. 
Published: April 2022

AbstractBackgroundTuberculosis is a leading infectious cause of death worldwide. Novel vaccines will be required to reach global targets and reverse setbacks from the COVID-19 pandemic. We estimated the impact of novel tuberculosis vaccines in low- and middle-income countries (LMICs) under alternative delivery scenarios.MethodsWe calibrated a tuberculosis model to 105 LMICs (93% of global tuberculosis incidence). Vaccine scenarios were implemented asBasecase: routine vaccination of 9-year-olds and a one-time vaccination campaign for ages ≥10 with country-specific introduction between 2028–2047 and 5-year scale-up to target coverage;Accelerated Scale-up: asBasecase, but all countries introducing in 2025 with instant scale-up; andRoutine Only: asBasecase, but routine vaccination only. Vaccines protected against disease for 10-years, with 50% efficacy.FindingsTheBasecasescenario prevented 44.0 (95% uncertainty range=37.2–51.6) million tuberculosis cases, and 5.0 (4.6–5.4) million tuberculosis deaths before 2050, including 2.2 million in the WHO South-East Asian region. TheAccelerated Scale-upscenario prevented 65.5 (55.6–76.0) million cases and 7.9 (7.3–8.5) million deaths before 2050. TheRoutine Onlyscenario prevented 8.8 (7.6–10.1) million cases and 1.1 (0.9–1.2) million deaths before 2050.InterpretationsNovel tuberculosis vaccines could have substantial impact, which will vary depending on delivery strategy. Including a campaign will be crucial for rapid impact. Accelerated introduction, more similar to the pace of COVID-19 vaccines, could increase lives saved by around 60%. Investment is required to support vaccine development, manufacturing, prompt introduction and scale-up.FundingWHO (2020/985800-0)
The bacterial lectin LecA from P. aeruginosa alters membrane organization by dispersing ordered domains
Authors: Sych Taras , Omidvar Ramin , Ostmann Rafael , Schubert Thomas , Brandel Annette , Richert Ludovic , Mely Yves , Madl Josef , Römer Winfried 
Published: April 2022

AbstractThe assembly and dynamic reorganization of plasma membrane nanodomains (also known as “lipid rafts”) play key roles in host cell infection by human pathogens (e.g. viruses and bacteria). Viruses and bacteria can trigger the reorganization of lipid rafts which leads to membrane invaginations and downstream signaling that promote infection. Such reorganizations can be induced by interactions of bacterial or viral carbohydrate proteins (so-called lectins) with lipid raft glycosphingolipids (GSLs). Here, we studied the GSL globotriaosylceramide (Gb3) which is a key receptor involved in the cellular uptake of the gram-negative bacterium P. aeruginosa. The bacterial surface lectin LecA targets Gb3 and promotes bacterial invasion via the “lipid zipper” mechanism. However, the impact of LecA on the organization of membrane nanodomains is unknown yet. We mimicked of the plasma membrane using supported lipid bilayers (SLBs) that contained liquid-ordered (Lo, “raft-like”, enriched in sphingolipids and GSLs) and liquid-disordered (Ld, “non-raft-like” enriched in DOPC) lipid domains. Upon interaction with LecA, the Lo domains in the SLBs reshaped and dispersed. Moreover, deformation of SLBs was observed as LecA formed membrane multilayers on SLBs surface. We further dissected this process to reveal the impact of Gb3 structure, bilayer composition and LecA valence on the Lo reorganization.
Diversity chromosome evolution of Ty1-copia Retrotransposons in Pennisetum purpureum Revealed by FISH
Authors: Yu Zehuai , Huang Yongji , Li Xueting , Wu Jiayun , Zhang Muqing , Deng Zuhu 
Published: April 2022

AbstractPennisetum purpureum is a potential species for biofuel production. Characterization and chromosomal distribution of retrotransposons could enhance the comprehension of the role and dynamics of the repetitive elements in plants. In this study, a phylogenetic tree was constructed according to the conserved reverse transcriptase sequences and revealed that these Ty1-copia retrotransposons had typical structure. Analysis showed that total Ty1-copia retrotransposons had a significant component, as high as 5.12 × 103 copy numbers in P. purpureum. Then, the chromosomal pattern of four known lineages were also analyzed with Pennisetum glaucum genome, which suggested that the Sire/Maximus lineage had the highest copy number and followed by Tork/Angela, Tork/TAR, Retrofit/Ale. Additionally, the chromosomal distribution of total Ty1-copia retrotransposons was detected by fluorescence in situ hybridization (FISH) to be a dispersed pattern with weak clustering, mostly near the centromeric regions of P. purpureum chromosomes; interestingly, there were four obvious signals in the subterminal chromosomes. These results suggested that there occurred differential dynamic evolution directions of Ty1-copia retrotransposons within P. purpureum. Furthermore, co-localization of Ty1-copia, 5S rDNA, and 35S rDNA indicated that two chromosome 2 and four chromosome 4 were identified. Concurrently, subterminal signals of Ty1-copia-type retrotransposons were located on four other homologous chromosomes. Altogether, these results shed light on the diversification of Ty1-copia retrotransposons and have the significance for generation of valid chromosomal markers in retrotransposon families.
Vibrio tarriae sp. nov., a novel member of the Cholerae clade isolated from across the United States
Authors: Islam Mohammad Tarequl , Liang Kevin , Orata Fabini D. , Im Monica S. , Alam Munirul , Lee Christine C. , Boucher Yann F. 
Published: April 2022

AbstractA number of bacteria with close resemblance to Vibrio cholerae has been isolated over the years by the Centers for Disease Control and Prevention (CDC), which could not be assigned a proper taxonomic designation based on preliminary identification methods. Nine such isolates have been found to share 16S rRNA gene identity exceeding 99% with V. cholerae, yet DNA-DNA hybridization (60.4-62.1%) and average nucleotide identity values (94.4-95.1%) were below the species cut-off, indicating a potentially novel species. Phylogenetic analysis of core genomes places this group of isolates in a monophyletic clade, within the “Cholerae clade,” but distinct from any other species. Extensive phenotypic characterization reveals unique biochemical properties that distinguish this novel species from V. cholerae. Comparative genomic analysis reveals a unique set of siderophore genes, suggesting that iron acquisition strategies could be vital for the divergence of the novel species from a common ancestor with V. cholerae. Based on genetic, phylogenetic, and phenotypic differences observed, we propose these isolates represent a novel species of the genus Vibrio, for which the name Vibrio tarriae sp. nov. is proposed. Strain 2521-89 (= DSM 112461 = CCUG 75318), isolated from lake water, is the type strain.Author NotesThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 2521-89 is MW773202.1. The genome sequences (genome assemblies) of strains 2521-89, 2523-88, 2015V-1076, 2016V-1018, 2016V-1062, 2017V-1038, 2017V-1070 and 2017V-1124 are deposited under the accession number NZ_CP022353.1, QKKG01000001.1, QKKH00000000.1, QKKI00000000.1, QKKJ00000000.1, QKKK00000000.1, QKKM00000000.1, and QKKN00000000.1, respectively. All the whole genome sequences are deposited under bioproject ID: PRJNA391152.
Mycn regulates intestinal development through ribosomal biogenesis in a zebrafish model of Feingold syndrome 1
Authors: Li Yun-Fei , Cheng Tao , Zhang Ying-Jie , Huang Ying , Sun Xiao-Zhi , Wang Dan , Liu Xiang , Chen Dong , Jin Xi , Xu Peng-Fei 
Published: April 2022

AbstractFeingold syndrome type 1, caused by loss-of-function of MYCN, is characterized by varied phenotypes including esophageal and duodenal atresia. However, no adequate model exists for studying the syndrome’s pathological or molecular mechanisms, nor is there a treatment strategy. Here, we developed a zebrafish Feingold syndrome type 1 model with nonfunctional mycn, which had severe intestinal atresia. Single-cell RNA-seq identified a subcluster of intestinal cells was highly sensitive to Mycn, and impaired cell proliferation decreased the overall number of intestinal cells in the mycn mutant fish. Bulk RNA-seq and metabolomic analysis showed that expression of ribosomal genes was downregulated and amino acid metabolism was abnormal. Ribosomal profiling analysis showed decreases in free 40S, 60S, and 80S ribosome particles, which led to impaired translation in the mutant. Further, both L-leucine and Rheb, which can elevate translation via TOR pathway, rescued the intestinal phenotype of mycn mutant. In summary, by this zebrafish Feingold syndrome type 1 model, we found that disturbance of ribosomal biogenesis and blockage of protein synthesis during development are primary causes of the intestinal defect in Feingold syndrome type 1. Importantly, our work suggests that leucine supplementation may be a feasible and easy treatment option for this disease.
Factors influencing preparedness self-efficacy among Hispanics and Latinos in the United States
Authors: Rivera Jason D. 
Published: April 2022

PurposeCurrently there is a lack of information regarding factors that influence individuals' disaster preparedness self-efficacy among various minority groups in the US. This research seeks to start filling this gap of knowledge by exploring potential factors among Hispanics and Latinos.Design/methodology/approachThis research uses disaggregated data from the 2020 FEMA National Household Survey to explore potential factors associated with preparedness self-efficacy among Hispanics and Latinos in the US. The study uses an ordered logistic regression to analyze data.FindingsThis research finds that Hispanics' and Latinos' preparedness self-efficacy is statistically related to their income and the FEMA region in which they live, but not prior disaster experience nor exposure and access to disaster preparedness information.Originality/valueThis paper is unique because of the current lack of information available on what specifically contributes to Hispanics' and Latinos' preparedness self-efficacy.
Barriers to healthcare for Venezuelan migrants: physicians' perspective
Authors: Modeste-James Akeem , Huggins Camille 
Published: April 2022

PurposeIncreased migration to small island developing states poses major challenges on receiving countries' healthcare systems. Due to public health policy on treating migrants in Trinidad and Tobago, Venezuelan migrants are experiencing less than optimal care. This paper examines the retrospective accounts of physicians treating Venezuelan migrants with limited access to healthcare.Design/methodology/approachTen in depth, semi-structured interviews were conducted with physicians working in primary healthcare and emergency departments about their clinical decision-making process in providing care for Venezuelan migrants. A narrative approach was used to highlight the personal experiences of participants. Participants were recruited by their response to an online advertisement on social media (i.e. Facebook and Instagram) and via email invitations. Interviews were transcribed verbatim and coded using NVIVO-12 software.FindingsFindings revealed language barriers between Venezuelan migrants and Trinidad and Tobago-based physicians hindered providing optimal care coordination. Physicians indicated the use of a translator app to bridge the service gap but questioned their ethics. Participants noted tensions between junior and senior physicians regarding referrals of Venezuelan migrants to outpatient care. The data suggests physicians' felt constrained providing additional services such as primary care due to the Provision of Public Healthcare Services Policy which forbids primary care services to migrants.Originality/valueSparse research on the barriers to accessing healthcare for Venezuelan migrants residing in the small island states.
Impact of Leveraged Lending Guidance: evidence from nonbank participation in syndicated loans
Authors: Schenck Natalya , Shi Lan 
Published: April 2022

Purpose
The purpose of this study is to examine the impact of supervisory Leveraged Lending Guidance (LLG) (2013–2014) on risk and structure of syndicated loans arranged by the largest US banks with participation of nonbank lenders.

Design/methodology/approach
This study uses supervisory shared national credit loan-level data from 2010 to 2015 and DealScan loan origination data and use linear regressions with clustered standard errors.

Findings
This study finds that the impact of the LLG was mixed. Incidence and risk of leveraged lending declined following the Guidance, as reflected in lower nonbank syndicate participation. However, the covenant protections weakened and loan spreads at origination declined. This study also provides evidence that some risky lending originations shifted to nonbank entities outside of the banking regulatory environment.

Originality/value
This study contributes and expands literature on the impact of regulatory guidance on loan risk, terms and structure, focusing on nonbank participation in syndicated commercial loans.
Working capital management, financial performance and growth of firms: empirical evidence from India
Authors: Sawarni Kumar Sanjay , Narayanasamy Sivasankaran , Chattopadhyay Subir , Chakrabarti Prasenjit 
Published: April 2022

Purpose
This paper aims to investigate how firms growing at a high average rate over a period differ in their working capital management (WCM) efficiency from those growing at a low rate during the same period. It also examines how WCM efficiency impacts firms’ financial performance and how firms’ growth influences this relationship.

Design/methodology/approach
The authors have analyzed the difference in WCM efficiency of a sample of 431 nonfinancial firms during 2012 to 2019 by segregating them into above median growth (AMG) and below median growth (BMG) firms. The authors have used fixed effect regression to investigate the impact of cash conversion cycle, inventory days, accounts receivable days and accounts payable days on the financial performance and the effect of growth on this relationship.

Findings
This study finds that AMG firms manage their working capital significantly more efficiently than BMG firms. It also reports that the WCM efficiency impacts the profitability and valuation of firms positively; however, this relationship is more intense for firms growing at a high rate than for those growing at a low rate.

Originality/value
This research should contribute to the less researched area of WCM by finding the effect of growth on the relationship between WCM efficiency and performance. The evidence found in this study may be of interest for industry practitioners and managers in identifying WCM efficiency as an important driver for the financial performance of their firms.
Viewpoint: applying pragmatism to stimulate service research and practice – a European perspective
Authors: Heinonen Kristina , Strandvik Tore 
Published: April 2022

Purpose
The purpose of this paper is to apply pragmatism – a philosophy of science on the interplay of human actions and meaning – as a perspective for studying service research and practice, emphasizing the need to deal with dynamics and diversity to cope with service marketplace disruptions. This work focuses on customers (individuals or groups of individuals) as key marketplace stakeholders.

Design/methodology/approach
Pragmatism provides a foundation for theorizing about change by connecting human actors’ cognitive belief structures and their actions through a continuous learning process. This paper outlines how the key principles of pragmatism can advance service research and practice.

Findings
Adopting the key principles of pragmatism in service management directs attention to service market dynamics. Understanding customers’ everyday lives as the interplay of experiencing, knowing and acting reveals insights about the role of service in dynamic markets for the benefit of service research and practice.

Research limitations/implications
The paper is a viewpoint to stimulate researchers’ reflections on often hidden core assumptions about service. Pragmatism provides a perspective on actors’ practical rationality and problem solving in dynamic settings. Along with its emphasis on a holistic understanding of customers’ lives, this perspective provides direction for future service research and practice. Further, conceptual development and empirical substantiation are encouraged.

Practical implications
By focusing on marketplace changes, this paper addresses management concerns for commercial and non-commercial organizations. Pragmatism encourages critical reflections on what companies are doing and why (the connection between actions and beliefs), revealing underlying beliefs and institutionalized industry practices that require modifications.

Social implications
Pragmatism is an approach to service research and practice, irrespective of aggregation level and sector. Therefore, it can help stimulate societal welfare.

Originality/value
Pragmatism advances service research by delineating a holistic perspective on customers’ lives and providing a perspective for exploring and understanding dynamics and diversity in service markets.
Effect of building orientations and heat treatments on AlSi10Mg alloy fabricated by selective laser melting: microstructure evolution, mechanical properties, fracture mechanism and corrosion behavior
Authors: Tarakçı Gürkan , Khan Hamaid Mahmood , Yılmaz Mustafa Safa , Özer Gökhan 
Published: April 2022

Purpose
The present paper aims to systematically investigate the influence of building orientations (0°, 15°, 30°, 45°, 60°, 75°) and heat treatment processes on the macro-/micro-structural, mechanical and electrochemical behaviors of selective laser melting (SLM) prepared AlSi10Mg alloy parts.

Design/methodology/approach
AlSi10Mg samples were produced by the SLM method using standard processing parameters at 0°, 15°, 30°, 45°, 60° and 75° building angles. The effects of building orientations on the physical, mechanical and electrochemical properties of the alloy were investigated.

Findings
With the increase in the building orientation from 15° to 75°, the structural defects were found reducing. The effect of step size of inclined geometries was found to significantly influence the mechanical and electrochemical properties of the AlSi10Mg samples. Tensile strength for samples fabricated at lower angles (0°, 15°, 30°) reported a drop of approximately 11% than SLM 0° samples. Moreover, the tensile strength was found to decrease from 412.35 ± 9.568 MPa for the as-built samples to 290.48 ± 12.658 MPa, whereas the fracture strain increases from 3.32 ± 0.56% to 5.6 ± 0.6% when the as-built sample was treated with T6 treatment. This study indicates that the microstructure and mechanical properties of SLM-processed AlSi10Mg alloy can be tailored by a suitable heat treatment or building angle.

Originality/value
Microstructural and mechanical behavior of horizontal or vertically built SLM components have already been demonstrated several times. However, the influence of different building orientations, such as 0°, 15°, 30°, 45°, 60°, 75°, has not been explored in-depth, particularly on corrosion and general mechanical performance. As a result, this work may be of significant relevance to academics and designers, given the varying orientation of internal component of SLM structures.