bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carm1 regulates the speed of C/EBPα-induced transdifferentiation by a cofactor stealing mechanism
Authors: Garcia Guillem Torcal , Kowenz-Leutz Elisabeth , Tian Tian V. , Klonizakis Antonios , Lerner Jonathan , de Andrés-Aguayo Luisa , Berenguer Clara , Plana-Carmona Marcos , Vila-Casadesús Maria , Bulteau Romain , Francesconi Mirko , Peiró Sandra , Zaret Kenneth S. , Leutz Achim , Graf Thomas 
Published: October 2022

ABSTRACTCell fate decisions are driven by lineage-restricted transcription factors but how they are regulated is incompletely understood. The C/EBPα-induced B cell to macrophage transdifferentiation (BMT) is a powerful system to address this question. Here we describe that C/EBPα with a single arginine mutation (C/EBPαR35A) induces a dramatically accelerated BMT in mouse and human cells. Changes in the expression of lineage-restricted genes occur as early as within 1 hour compared to 18 hours with the wild type. Mechanistically C/EBPαR35Aexhibits an increased affinity for PU.1, a bi-lineage transcription factor required for C/EBPα-induced BMT. The complex induces more rapid chromatin accessibility changes and an enhanced relocation (stealing) of PU.1 from B cell to myeloid gene regulatory elements. Arginine 35 is methylated by Carm1 and inhibition of the enzyme accelerates BMT, similar to the mutant. Our data suggest that the relative proportions of methylated and unmethylated C/EBPα in a bipotent progenitor can determine the velocity of cell fate choice and lineage directionality.
Nonsense-mediated mRNA decay of metal-binding activator MAC1 is dependent on copper levels and 3′-UTR length in Saccharomyces cerevisiae
Authors: Zhang Xinyi , Kebaara Bessie W. 
Published: October 2022

ABSTRACTThe nonsense-mediated mRNA decay (NMD) pathway was initially identified as a pathway that degrades mRNAs with premature termination codons but is now also recognized as a post-transcriptional gene regulatory pathway which regulates expression of natural mRNAs. Earlier studies demonstrated that regulation of functionally related mRNAs by NMD can be differential and conditional in Saccharomyces cerevisiae. Here, we elucidate the regulation of MAC1 mRNAs by NMD in response to copper, and the role MAC1 3′-UTR plays in this regulation. MAC1 is a copper-sensing transcription factor that regulates high affinity copper transport and is activated under low copper conditions in S. cerevisiae. We found that MAC1 mRNAs were regulated by NMD under normal growth conditions but escape NMD under low and high copper conditions. Mac1p regulated genes escape NMD in conditions where MAC1 mRNAs are NMD sensitive. We also found that the MAC1 3′-UTR contributes to the degradation of the mRNAs by NMD and that MAC1 mRNAs lacking 3′-UTR were stabilized in response to copper deprivation. Taken together, our results demonstrate a novel mechanism of regulating a metal sensing transcription factor, where MAC1 mRNA levels are regulated by NMD and copper, while the activity of Ma1p is controlled by copper levels.
An agent-based modeling approach for lung fibrosis in response to COVID-19
Authors: Islam Mohammad Aminul , Getz Michael , Macklin Paul , Versypt Ashlee N. Ford 
Published: October 2022

AbstractThe severity of the COVID-19 pandemic has created an emerging need to investigate the long-term effects of infection on patients. Many individuals are at risk of suffering pulmonary fibrosis due to the pathogenesis of lung injury and impairment in the healing mechanism. Fibroblasts are the central mediators of extracellular matrix (ECM) deposition during tissue regeneration, regulated by anti-inflammatory cytokines including transforming growth factor beta (TGF-β). The TGF-β-dependent accumulation of fibroblasts at the damaged site and excess fibrillar collagen deposition lead to fibrosis. We developed an open-source, multiscale tissue simulator to investigate the role of TGF-β sources in the progression of lung fibrosis after SARS-CoV-2 exposure, intracellular viral replication, infection of epithelial cells, and host immune response. Using the model, we predicted the dynamics of fibroblasts, TGF-β, and collagen deposition for 15 days post-infection in virtual lung tissue. Our results showed variation in collagen area fractions between 2% and 40% depending on the spatial behavior of the sources (stationary or mobile), the rate of activation of TGF-β, and the duration of TGF-β sources. We identified M2 macrophages as primary contributors to higher collagen area fraction. Our simulation results also predicted fibrotic outcomes even with lower collagen area fraction when spatially-localized latent TGF-β sources were active for longer times. We validated our model by comparing simulated dynamics for TGF-β, collagen area fraction, and macrophage cell population with independent experimental data from mouse models. Our results showed that partial removal of TGF-β sources changed the fibrotic patterns; in the presence of persistent TGF-β sources, partial removal of TGF-β from the ECM significantly increased collagen area fraction due to maintenance of chemotactic gradients driving fibroblast movement. The computational findings are consistent with independent experimental and clinical observations of collagen area fractions and cell population dynamics not used in developing the model. These critical insights into the activity of TGF-β sources may find applications in the current clinical trials targeting TGF-β for the resolution of lung fibrosis.Author summaryCOVID-19 survivors are at risk of lung fibrosis as a long-term effect. Lung fibrosis is the excess deposition of tissue materials in the lung that hinder gas exchange and can collapse the whole organ. We identified TGF-β as a critical regulator of fibrosis. We built a model to investigate the mechanisms of TGF-β sources in the process of fibrosis. Our results showed spatial behavior of sources (stationary or mobile) and their activity (activation rate of TGF-β, longer activation of sources) could lead to lung fibrosis. Current clinical trials for fibrosis that target TGF-β need to consider TGF-β sources’ spatial properties and activity to develop better treatment strategies.
Diverging Neural Dynamics for Syntactic Structure Building in Naturalistic Speaking and Listening
Authors: Giglio Laura , Ostarek Markus , Sharoh Daniel , Hagoort Peter 
Published: October 2022

AbstractThe neural correlates of sentence production have been mostly studied with constraining task paradigms that introduce artificial task effects. In this study, we aimed to gain a better understanding of syntactic processing in spontaneous production vs. naturalistic comprehension. We extracted word-by-word metrics of phrase-structure building with top-down and bottom-up parsers that make different hypotheses about the timing of structure building. In comprehension, structure building proceeded in an integratory fashion and led to an increase in activity in posterior temporal and inferior frontal areas. In production, structure building was anticipatory and predicted an increase in activity in the inferior frontal gyrus. Newly developed production-specific parsers highlighted the anticipatory and incremental nature of structure building in production, which was confirmed by a converging analysis of the pausing patterns in speech. Overall, the results showed that the unfolding of syntactic processing diverges between speaking and listening.
Distinct phenotype of SARS-CoV-2 Omicron BA.1 in human primary cells but no increased host range in cell lines of putative mammalian reservoir species
Authors: Essaidi-Laziosi Manel , Perez Rodriguez Francisco Javier , Alvarez Catia , Sattonnet-Roche Pascale , Torriani Giulia , Bekliz Meriem , Adea Kenneth , Lenk Matthias , Suliman Tasnim , Preiser Wolfgang , Müller Marcel A. , Drosten Christian , Kaiser Laurent , Eckerle Isabella 
Published: October 2022

AbstractSARS-CoV-2’s genetic plasticity has led to several variants of concern (VOCs). Here we studied replicative capacity for seven SARS-CoV-2 isolates (B.1, Alpha, Beta, Gamma, Delta, Zeta, and Omicron BA.1) in primary reconstituted airway epithelia (HAE) and lung-derived cell lines. Furthermore, to investigate the host range of Delta and Omicron compared to ancestral SARS-CoV-2, we assessed replication in 17 cell lines from 11 non-primate mammalian species, including bats, rodents, insectivores and carnivores. Only Omicron’s phenotype differedin vitro, with rapid but short replication and efficient production of infectious virus in nasal HAEs, in contrast to other VOCs, but not in lung cell lines. No increased infection efficiency for other species was observed, but Delta and Omicron infection efficiency was increased in A549 cells. Notably replication in A549 and Calu3 cells was lower than in nasal HAE. Our results suggest better adaptation of VOCs towards humans, without an extended host range.
In vivo generation of heart and vascular system by blastocyst complementation
Authors: Coppiello Giulia , Barlabé Paula , Moya-Jódar Marta , Abizanda Gloria , Barreda Carolina , Iglesias Elena , Linares Javier , Arellano-Viera Estibaliz , Ruiz-Villalba Adrian , Larequi Eduardo , Carvajal-Vergara Xonia , Pelacho Beatriz , Prósper Felipe , Aranguren Xabier L. 
Published: October 2022

SUMMARYThe generation of organs from stem cells by blastocyst complementation is a promising approach to cover the clinical need for transplants. In order to generate rejection-free organs, complementation of both parenchymal and vascular cells must be achieved, as endothelial cells play a key role in graft rejection. Here we used a lineage-specific cell ablation system to produce mouse embryos unable to form both the cardiac and vascular systems. By mouse intraspecies blastocyst complementation we rescued heart and vascular development separately and in combination, obtaining complemented hearts with cardiomyocytes and endothelial cells of exogenous origin. Complemented chimeras were viable and reached adult stage, showing normal cardiac function and no signs of histopathological defects in the heart. Furthermore, we implemented the cell ablation system for rat-to-mouse blastocyst complementation, obtaining xenogeneic hearts whose cardiomyocytes were completely of rat origin. These results represent an advance in the experimentation towards the in vivo generation of transplantable organs.
SARS-CoV-2 ORF3c impairs mitochondrial respiratory metabolism, oxidative stress and autophagic flow
Authors: Mozzi Alessandra , Oldani Monica , Forcella Matilde E. , Vantaggiato Chiara , Cappelletti Gioia , Pontremoli Chiara , Valenti Francesca , Forni Diego , Biasin Mara , Sironi Manuela , Fusi Paola , Cagliani Rachele 
Published: October 2022

AbstractCoronaviruses encode a variable number of accessory proteins that play a role in host-virus interactions, in the suppression of immune responses, or in immune evasion. Accessory proteins in SARS-CoV-2 consist of at least twelve viral proteins whose roles during infection have been extensively studied. Nevertheless, the role of the ORF3c accessory protein, an alternative open reading frame of ORF3a, has remained elusive. Herein, we characterized ORF3c in terms of cellular localization, host’s antiviral response modulation, and effects on mitochondrial metabolism. We show that ORF3c has a mitochondrial localization and alters mitochondrial metabolism, resulting in increased ROS production, block of the autophagic flux, and accumulation of autophagosomes/autolysosomes. Notably, we also found that ORF3c induces a shift from glucose to fatty acids oxidation and enhanced oxidative phosphorylation. This is similar to the condition observed in the chronic degenerative phase of COVID-19. Altogether these data suggest that ORF3c could be a key protein for SARS-CoV-2 pathogenesis and that it may play a role in disease progression.
Orchestration and consolidation in corporate sustainability reporting. The legacy of the Corporate Reporting Dialogue
Authors: Rowbottom N. 
Published: October 2022

PurposeThe paper uses theoretical conceptions of power and orchestration to analyse the role of the Corporate Reporting Dialogue on the global standardisation of sustainability reporting.Design/methodology/approachThe paper adopts an interpretive approach and draws on a qualitative dataset derived from interviews, documentary analysis and observation.FindingsThe paper traces how the Corporate Reporting Dialogue was orchestrated by the International Integrated Reporting Council, with the objective of aligning sustainability reporting standards, but moved to become a vehicle for orchestrating standards consistent with the recommendations of the Task Force for Climate-Related Financial Disclosure. Collaboration between the Dialogue's five most active bodies forged the blueprint adopted by the International Sustainability Standards Board's vision of sustainability reporting that prioritised reporting only on those socio-ecological issues deemed to materially affect future enterprise value.Originality/valueThe paper explicates the role of collaborative initiatives in the standardisation of sustainability reporting and shows how these initiatives act as vehicles to subtly undermine the GRI position (presented as one standardiser amongst many whose vision appears as an outlier, despite its position as the dominant sustainability reporting standardiser), and establish the prioritisation of a sustainability reporting worldview based on investor-oriented enterprise value creation. The case also draws attention to the specific orchestrators involved in establishing this prioritisation, and reveals the influence of philanthropic foundations. In doing so, it extends our understanding of legitimacy generation in standard-setting by showing how collaborative initiatives offer private standardisers another means to generate input legitimacy for what, in this case, represented a vision of reporting at odds with most sustainability reporting practice. Finally, the paper extends the sites of power to collaborative initiatives and details the mechanisms through which covert power is exercised but also masked where orchestrators use convening power, funding and membership choices to define the boundaries of discussion by influencing who participates, what is on the agenda and what activity is undertaken. Rather than viewing standardisation as a simple pursuit of conquest between individual standardisers, the paper considers how collaboration provides the opportunity for assimilation.
Cultural murals and the evolving nature of the hero concept: an arts marketing context
Authors: Fillis Ian , Lehman Kim 
Published: October 2022

PurposeThe authors provide an understanding of how the hero identity is culturally constructed and evolving. The authors focus on heroism within an arts marketing framework through an interrogation of Northern Ireland murals. In this paper, the authors elaborate on the links between arts marketing thought and the notion of hero and draw conclusions around what the authors see as a fruitful area for arts marketing theory.Design/methodology/approachThe authors have adopted a narrative approach, incorporating biographical method, visual analysis and ethnography in interpreting cultural murals. The authors assess representative examples in Northern Ireland using a thematic framework.FindingsThe murals the authors assessed have evolved from having a specific community focus to increasing numbers which now represent a “shared”, and therefore more modern version of the hero.Research limitations/implicationsThe authors identify an emerging, aesthetically balanced portrayal of cultural murals, with a different set of heroic priorities compared to the past, which should encourage further related research elsewhere.Practical implicationsNorthern Ireland murals are no longer the preserve of specific communities and are now also shared spaces which appeal to both the local population and cultural tourists.Originality/valueAlthough analysis and evaluation of political murals has been carried out in other disciplines, the authors add to the limited insight from an arts marketing perspective.
Impact of climate change on agricultural productivity: a combination of spatial Durbin model and entropy approaches
Authors: Bai Dongbei , Ye Lei , Yang ZhengYuan , Wang Gang 
Published: October 2022

Purpose
Global climate change characterized by an increase in temperature has become the focus of attention all over the world. China is a sensitive and significant area of global climate change. This paper specifically aims to examine the association between agricultural productivity and the climate change by using China’s provincial agricultural input–output data from 2000 to 2019 and the climatic data of the ground meteorological stations.

Design/methodology/approach
The authors used the three-stage spatial Durbin model (SDM) model and entropy method for analysis of collected data; further, the authors also empirically tested the climate change marginal effect on agricultural productivity by using ordinary least square and SDM approaches.

Findings
The results revealed that climate change has a significant negative effect on agricultural productivity, which showed significance in robustness tests, including index replacement, quantile regression and tail reduction. The results of this study also indicated that by subdividing the climatic factors, annual precipitation had no significant impact on the growth of agricultural productivity; further, other climatic variables, including wind speed and temperature, had a substantial adverse effect on agricultural productivity. The heterogeneity test showed that climatic changes ominously hinder agricultural productivity growth only in the western region of China, and in the eastern and central regions, climate change had no effect.

Practical implications
The findings of this study highlight the importance of various social connections of farm households in designing policies to improve their responses to climate change and expand land productivity in different regions. The study also provides a hypothetical approach to prioritize developing regions that need proper attention to improve crop productivity.

Originality/value
The paper explores the impact of climate change on agricultural productivity by using the climatic data of China. Empirical evidence previously missing in the body of knowledge will support governments and researchers to establish a mechanism to improve climate change mitigation tools in China.
Corporate entrepreneurship champions: mapping the past and present states of the field for future advancements
Authors: Ghura Amarpreet Singh , Sharma Gagan Deep , Pereira Vijay , Islam Nazrul , Chopra Ritika 
Published: October 2022

PurposeThe purpose of this study is to critically examine and review the extant research on corporate entrepreneurship champions in the broader area of corporate entrepreneurship and to uncover the avenues for advancement of the scholarship with the purpose of engaging CE champions towards the upliftment of organisations in particular, and younger workforce in general.Design/methodology/approachIn this study, authors employ bibliometric analysis through a review of 274 papers fetched from Web of Science and Scopus databases.FindingsThe authors set the agenda for future research and policy by elucidating research themes and potential research questions by bringing out twelve themes classified into five basic themes, three niche themes, three motor themes, and one key theme, while also providing the methodological inputs for carrying out this agenda.Originality/valueThis study adopts a unique lens of investigation in contextualising the role of self-efficacy, employee engagement, and career choice for the younger workforce.
Student roles and behaviors in higher education co-creation – a systematic literature review
Authors: Zarandi Negin , Soares Ana Maria , Alves Helena 
Published: October 2022

PurposeIn today’s global and highly competitive climate among universities, educational developers and instructors have focused more on trying to make the student experience more engaging. In this manner, student co-creation activities have recently become a major research priority in marketing and higher education (HE) research. The purpose of this study is to present a systematic review of the literature on student co-creation roles and behaviors in HE in order to map extant research on this topic and offer a consolidated view of the co-creation process and approaches that can be employed by HEIs to motivate students to co-create their HE experience.Design/methodology/approachA Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) approach was followed to classify, select, synthesize, analyze and assess the most relevant studies on student participation in co-creation in HE.FindingsThis study’s analysis has identified that the co-creation process in HE includes dialog, access, risk and transparency. The main approaches used by higher education institutions (HEIs) to motivate students to co-create their HE experience are student involvement, cognitive engagement, university affiliation and emotional engagement. Our review also shows that student co-creation behaviors are mainly participation and citizenship behavior, and their co-creation roles include those of co-producers, participants, change agents and partners.Originality/valueThis systematic literature review analyses and critically discusses the state of the art in student co-creation roles in HE and the approaches HEIs use. By providing a map of existing research, the paper contributes both to the clarification of student co-creation roles and behaviors in HE and the identification of research gaps and opportunities for further research.
The importance of community support for women in a Gulf Coast Indigenous tribe
Authors: Carlson Tess A. , Liddell Jessica L. 
Published: October 2022

Purpose
Community support is an integral aspect of health and well-being for Indigenous peoples. The purpose of this paper is to demonstrate the valuable role of community support for Indigenous women specifically, who experience reproductive health disparities at alarming rates. This study helps fill an important gap in Indigenous scholarship by centering the resilience of women and Indigenous tribes and by using a framework that is consistent with Indigenous holistic views of health.

Design/methodology/approach
The data for this paper was collected as part of a larger study exploring the reproductive health experiences of a state-recognized Gulf Coast tribe. A total of 31 semi-structured interviews were conducted with individuals who identify as women and as members of this tribe using qualitative descriptive methodology. This method is recommended for research with Indigenous communities. A community advisory board with representatives from this tribe provided feedback throughout the project.

Findings
Themes expressed by participants included Community Closeness and Support; Community Support in Raising Children; Informal Adoption Common; and Community Values of Mutual Aid and Self-Sufficiency. The findings support current literature noting the value of generational and communal ties for Indigenous peoples. Implications of this research include the need to value and support community networks in programs serving tribes, in addition to meaningfully including Indigenous communities in developing interventions.

Originality/value
This paper centers Indigenous women’s resilience, approaches the health and well-being of Indigenous tribes holistically and helps to fill an important gap in literature describing informal adoption (outside the legal system) in state-recognized Indigenous communities.