bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression
Authors: Liu Wentao , Ren Dianyun , Xiong Wei , Jin Xin , Zhu Liang 
Published: January 2022

AbstractBackgroundTyrosine kinase inhibitors (TKIs) alone and in combination with immune checkpoint inhibitors (ICIs) have been shown to be beneficial for the survival of metastatic renal cell carcinoma (mRCC) patients, but resistance to targeted therapy and ICIs is common in the clinic. Understanding the underlying mechanism is critical for further prolonging the survival of renal cancer patients. Nuclear factor of activated T cell 1 (NFAT1) is expressed in immune and nonimmune cells, and the dysregulation of NFAT1 contributes to the progression of various type of malignant tumors. However, the specific role of NFAT1 in RCC is elusive. As a regulator of the immune response, we would like to systemically study the role of NFAT1 in RCC.MethodsTCGA-KIRC dataset analysis, Western blot analysis and RT-qPCR analysis was used to determine the clinic-pathological characteristic of NFAT1 in RCC. CCK-8 assays, colony formation assays and xenograft assays were performed to examine the biological role of NFAT1 in renal cancer cells. RNA-seq analysis was used to examine the pathways changed after NFAT1 silencing. ChIP-qPCR, coimmunoprecipitation analysis, Western blot analysis and RT-qPCR analysis were applied to explore the mechanism by NAFT1 was regulated in the renal cancer cells.ResultsIn our study, we found that NFAT1 was abnormally overexpressed in RCC and that NFAT1 overexpression was associated with an unfavorable prognosis. Then, we showed that NFAT1 enhanced tumor growth and regulated the immune response by increasing PD-L1 expression in RCC. In addition, we demonstrated that NFAT1 was stabilized in sunitinib-resistant RCC via hyperactivation of the PI3K/AKT/GSK-3β signaling pathway. Furthermore, our study indicated that downregulation of the expression of FBW7, which promotes NFAT1 degradation, was induced by FOXA1 and SETD2 in sunitinib-resistant RCC. Finally, FBW7 was found to contribute to modulating the immune response in RCC.ConclusionsOur data reveal a novel role for the FBW7/NFAT1 axis in the RCC response to TKIs and ICIs. NFAT1 and its associated signaling pathway might be therapeutic targets for RCC treatment, especially when combined with ICIs and/or TKIs.
Leveraging quorum sensing system for automatic coordination of Escherichia coli growth and lactic acid biosynthesis
Authors: Ge Chang , Run Shunqi , Jia Hongkai , Tian Pingfang 
Published: January 2022

Abstract
Purpose
Overproduction of desired metabolites usually sacrifices cell growth. Here we report that quorum sensing (QS) can be exploited to coordinate cell growth and lactic acid production in Escherichia coli.

Methods
We engineered two QS strains: one strain overexpressing acyl-homoserine lactone (AHL) synthesis genes (“ON”), the other strain overexpressing both AHL synthesis and degradation gene (aiiA) (“ON to semi-OFF”). To clarify the impact of the QS system on lactic acid production, D-lactate dehydrogenase gene ldhA was deleted from the E. coli genome, and Enhanced Green Fluorescence Protein (eGFP) was used as the reporter.

Results
Compared to the “ON” strain, the “ON to semi-OFF” strain showed delayed log growth and decreased egfp expression at stationary phase. When egfp was replaced by ldhA for lactic acid production, compared to the wild-type strain, the “ON to semi-OFF” strain demonstrated 231.9% and 117.3% increase in D-lactic acid titer and space-time yield, respectively, while the “ON” strain demonstrated 83.6%, 31%, and 36% increase in growth rate, maximum OD600, and glucose consumption rate, respectively. Quantitative real-time PCR revealed that both ldhA and the genes for phosphotransferase system were up-regulated in ldhA-overexpressing “ON” strain compared to the strain only harboring QS system. Moreover, the “ON” strain showed considerable increase in glucose consumption after a short lag phase. Compared to the reference strain harboring only ldhA gene in vector, both the “ON” and “ON to semi-OFF” strains demonstrated synchronization between cell growth and D-lactic acid production.

Conclusions
Collectively, QS can be leveraged to coordinate microbial growth and product formation.
Vascular thromboses with retroperitoneal fibrosis: a case report
Authors: Charaf Hanane , Zahraoui Rachida , Soualhi Mouna , Rguig Nezha , Bourkadi Jamal Eddine , Mohamed Daoud Ali , Ittimad Nasser 
Published: January 2022

Abstract
Background
Granulomatosis with polyangiitis is a systemic inflammatory disease characterized by necrotizing vasculitis that affects small- and medium-sized blood vessels. Granulomatous inflammation affects the lungs, ears, nose, and throat, and commonly affects the kidneys, although the retroperitoneal tissue is rarely affected. Several studies have reported an increased risk of venous thromboembolism. Early diagnosis and treatment are of vital importance due to the rapid progression of the disease.

Case presentation
We present the case of a 66-year-old Moroccan man followed for bilateral jugular thrombosis. Cavitary pulmonary nodules and retroperitoneal fibrosis with thrombosis involving several vascular territories were detected on thoracoabdominopelvic computerized tomography scan. Laboratory analyses revealed that the patient was positive for cytoplasmic antineutrophilic antibodies. The diagnosis of granulomatosis with polyangiitis was retained. Treatment with glucocorticoids and immunosuppressive agents resulted in significant clinical and radiological improvement over the following months.

Conclusions
We describe the diagnostic steps and the difficulty of managing this patient. Rare manifestations, such as retroperitoneal fibrosis, have been reported in the literature in association with granulomatosis with polyangiitis, and should not delay the diagnosis and treatment of granulomatosis with polyangiitis owing to its severity.
Left supraclavicular (Virchow’s) node metastasis detected before primary infradiaphragmatic tumor: a case series
Authors: Mochizuki Yumi , Tsuchiya Maiko , Oyama Jun , Wada Akane , Kugimoto Takuma , Kuroshima Takeshi , Hirai Hideaki , Tomioka Hirofumi , Harada Hiroyuki , Ikeda Tohru , Akashi Takumi 
Published: January 2022

Abstract
Background
Metastasis of infradiaphragmatic tumors to the left supraclavicular lymph node is reported to be rare. When metastasis is detected in the left supraclavicular node in patients with head and neck carcinoma, locating the primary cancer remains a difficult and time-consuming challenge despite the dramatic development of screening technologies and treatment methods.

Case presentation
We report three cases of malignant infradiaphragmatic tumor diagnosed following an initial finding of left supraclavicular node metastasis after surgery for tongue squamous cell carcinoma (follow-up period, range 18–62 months). In these cases, adenocarcinoma was diagnosed based on left supraclavicular node biopsies, and a second primary tumor was found, in a 78-year-old Japanese woman with a diagnosis of cholangiocarcinoma, a 64-year-old Japanese man with a diagnosis of bladder carcinoma, and a 61-year-old Japanese man with a diagnosis of prostate carcinoma. In the cholangiocarcinoma case, carbohydrate antigen 19-9 and alpha-fetoprotein levels helped to diagnose cholangiocarcinoma. Palliative care only was given, with survival for 11 months after diagnosis of lymph node metastasis. In the bladder carcinoma case, pathological analysis of fine-needle aspiration biopsy specimen of the metastatic cervical lymph node showed atypical cells with slight squamous differentiation. Hematoxylin–eosin staining of the bladder lesion did not identify a clear glandular or squamous component, and we could not make a definitive diagnosis of whether the lesion was poorly differentiated squamous cell carcinoma, adenocarcinoma, or high-grade urothelial carcinoma. GATA3 staining aided in the diagnosis of urothelial bladder cancer with left supraclavicular node metastasis. He survived for 2 months after diagnosis of left supraclavicular lymph node metastasis. In the prostate carcinoma case, 18F‐fluorodeoxyglucose uptake was weak. Prostate-specific antigen levels and magnetic resonance imaging findings aided the diagnostic process. This patient underwent bilateral orchiectomy and adjuvant hormonal therapy and survived for 47 months after diagnosis of left supraclavicular node metastasis.

Conclusions
Pathological diagnosis on the basis of immunohistochemistry and specific diagnosis methods such as radiological and serological assessments are important for providing rapid diagnosis and appropriate treatment.
Modelling the potential risk zone of Lantana camara invasion and response to climate change in eastern India
Authors: Tiwari Sharad , Mishra S. N. , Kumar Dharmendra , Kumar Basant , Vaidya S. N. , Ghosh Brojo Gopal , Rahaman Sk Mujibar , Khatun Masjuda , Garai Sanjoy , Kumar Amit 
Published: January 2022

Abstract
Background
The aim of this study is to elucidate the potential risk zones prone to the invasion of perennial ornamental plant Lantana camara, which is native to South America and has invasive tendency in Jharkhand, eastern India, for present (2020) and the future (2050) climatic conditions under four different Representative Concentration Pathways scenarios (RCPs). We analysed the current distribution pattern of L. camara in the plateau region of eastern India and identified potentially suitable habitats prone to its further infestation in the future under the climate change scenario.

Results
Results showed the presence of L. camara invasion in ~ 13% of the geographical area of Jharkhand, Chotanagpur plateau which may expand up to 20–26% by 2050 depending upon emission scenarios as characterised by the four RCPs. Analysis for the current scenario suggests the dominance of L. camara in sub-zone V (12.77% under high risk zones (HRZ) and 9.5% under critical risk zones (CRZ)) followed by sub-zones IV (6.7%: HRZ; 4.19%: CRZ) and VI (2.49%: HRZ; 2.14%: CRZ). Future projection (2050) indicates a possible expansion of its distribution range across all agro-climatic sub-zones with dominance in sub-zones V and IV. Variable Bio_4 (temperature seasonality) was observed as the most contributing factor for the distribution of L. camara for current and future scenarios across all RCPs. Suitable habitat for L. camara mostly occurred under natural vegetation (66.05% of CRZ and 60.71% of HRZ) and agriculture landscape (29.51% of CRZ and 34.48% of HRZ).

Conclusions
The study provides an insight of invasion of L. camara in the plateau region of eastern India, and reveals wide distribution across all the agro-climatic sub-zones of Jharkhand, mostly in open and disturbed areas under natural vegetation and agriculture landscapes. Future projections for the year 2050 suggest a continuous increase in the expansion range of invasion across Jharkhand and call for urgent initiatives to combat its further invasion.
Influence of sports experience on distribution of pro-saccade reaction time under gap condition
Authors: Kunita Kenji , Fujiwara Katsuo 
Published: January 2022

Abstract
Background
Previous studies indicated that substantial individual variation exists in the distribution of pro-saccade reaction times under gap condition. To investigate the influence of sports experience on the distribution, we examined distribution of the pro-saccade reaction time under overlap and gap conditions, for the basketball club, table tennis club, and non-sporting control groups.

Methods
Subjects performed pro-saccade tasks under the overlap and gap conditions, in which the intentional and reflexive disengagement of fixation are important, respectively. Under the overlap condition, the central fixation point was illuminated for a random duration of 1–3 s, then the fixation point was turned off. Just after the switch-off of the fixation point, one of the peripheral targets was illuminated for a duration of 1 s. The visual stimulus under the gap condition was almost the same as that under the overlap condition. However, only the temporal gap between the switch-off of the fixation point and the onset of the target differed between those conditions. The gap duration in the gap condition was set at 200 ms. The mean of median value of the bandwidth showing the earliest peak in the histogram was calculated for each group. Thereafter, for each subject, the bandwidth showing the earliest peak under the gap condition was defined as the criterion bandwidth (0 ms bandwidth). Based on this criterion bandwidth, the mean of the relative frequency was calculated for every 10 ms of bandwidth, for the overlap and gap conditions, in each group.

Results
Under the overlap condition, for all subjects, the pro-saccade reaction times showed unimodal distribution. The means of the median value of the bandwidth showing the earliest peak for the basketball and table tennis groups (approximate 170 ms) were significantly earlier than that for the control group (approximate 190 ms). Under the gap condition, the distribution was bimodal for 11 of 15 subjects in the basketball group and for 5 of 15 subjects in the control group. In the table tennis group, the distribution was not bimodal but unimodal for all 15 subjects. For the basketball group, mean of the relative frequency showed bimodal distribution with approximate 120 ms and 170 ms peaks. For the table tennis and control groups, the mean of the relative frequency showed unimodal distribution with approximate 130 ms and 140ms peak, respectively.

Conclusions
The present study indicated that under the gap condition, the sports experience influenced on the distribution of the pro-saccade reaction time. The pro-saccade reaction time under the condition would show a distinct bimodal distribution for the basketball group and show a distinct and early unimodal distribution for the table tennis group. It was suggested that the physiological factor leading the group difference in the distribution was the effect of sports experience on the disengagement function of fixation.
Pathological complete response achieved by gemcitabine plus cisplatin therapy for initially unresectable advanced gallbladder cancer: a case report
Authors: Miura Yuya , Ashida Ryo , Sugiura Teiichi , Ohgi Katsuhisa , Yamada Mihoko , Otsuka Shimpei , Todaka Akiko , Uesaka Katsuhiko 
Published: January 2022

Abstract
Background
Conversion surgery for initially unresectable gallbladder cancer is rarely performed due to the low response rate for systemic chemotherapy, and a pathological complete response is seldom achieved.

Case presentation
A 67-year-old woman with jaundice was referred to our hospital and diagnosed with unresectable gallbladder cancer with extra-regional lymph node metastasis after examinations. After biliary decompression, gemcitabine plus cisplatin therapy was started. The tumor marker levels markedly decreased, and imaging studies revealed a reduction in the primary tumor and metastatic lymph nodes. The primary tumor and metastatic lymph node were still shrunk at 4 years after the start of gemcitabine plus cisplatin therapy, so we decided to perform conversion surgery. Gallbladder bed resection and lymph node dissection were performed. The pathological findings of the resected specimen showed only partial fibrosis in the gallbladder wall and no malignant findings in the dissected lymph nodes, indicating a pathological complete response. As of 24 months after the operation, she is alive without recurrence.

Conclusion
Although there have been only a few reports of conversion surgery for initially unresectable gallbladder cancer, it may be worthwhile to perform chemotherapy with the potential goal of subsequent conversion surgery.
Achieving a Carbon Neutral Future through Advanced Functional Materials and Technologies
Authors: Chapman Andrew , Ertekin Elif , Kubota Masanobu , Nagao Akihide , Bertsch Kaila , Macadre Arnaud , Tsuchiyama Toshihiro , Masamura Takuro , Takaki Setsuo , Komoda Ryosuke , Dadfarnia Mohsen , Somerday Brian , Staykov Alexander Tsekov , Sugimura Joichi , Sawae Yoshinori , Morita Takehiro , Tanaka Hiroyoshi , Yagi Kazuyuki , Niste Vlad , Saravanan Prabakaran , Onitsuka Shugo , Yoon Ki-Seok , Ogo Seiji , Matsushima Toshinori , Tumen-Ulzii Ganbaatar , Klotz Dino , Nguyen Dinh Hoa , Harrington George , Adachi Chihaya , Matsumoto Hiroshige , Kwati Leonard , Takahashi Yukina , Kosem Nuttavut , Ishihara Tatsumi , Yamauchi Miho , Saha Bidyut Baran , Islam Md Amirul , Miyawaki Jin , Sivasankaran Harish , Kohno Masamichi , Fujikawa Shigenori , Selyanchyn Roman , Tsuji Takeshi , Higashi Yukihiro , Kirchheim Reiner , Sofronis Petros 
Published: January 2022

Abstract
Current greenhouse gas emissions suggest that keeping global temperature increase below 1.5 degrees, as espoused in the Paris Agreements will be challenging, and to do so, the achievement of carbon neutrality is of utmost importance. It is also clear that no single solution can meet the carbon neutral challenge, so it is essential for scientific research to cover a broad range of technologies and initiatives which will enable the realization of a carbon free energy system. This study details the broad, yet targeted research themes being pioneered within the International Institute for Carbon-Neutral Energy Research (I2CNER). These approaches include hydrogen materials, bio-mimetic catalysts, electrochemistry, thermal energy and absorption, carbon capture, storage and management and refrigerants. Here we outline the state of the art for this suite of technologies and detail how their deployment, alongside prudent energy policy implementation can engender a carbon neutral Japan by 2050. Recognizing that just as no single technological solution will engender carbon neutrality, no single nation can expect to achieve this goal alone. This study represents a recognition of conducive international policy agendas and is representative of interdisciplinary, international collaboration.
Gait Biomechanics and Balance Associate with Talar and Subtalar T1ρ Relaxation Times in Those with Chronic Ankle Instability
Authors: WIKSTROM ERIK A. , SONG KYEONGTAK , TENNANT JOSHUA N. , PIETROSIMONE BRIAN 
Published: January 2022

ABSTRACT

Purpose
This study aimed to determine associations between T1ρ relaxation times of talar and subtalar articular cartilage and commonly altered gait biomechanics and postural control outcomes in those with chronic ankle instability (CAI).

Methods
Fifteen individuals with CAI (21.13 ± 1.81 yr) completed a T1ρ magnetic resonance imaging as well as a postural control and an overground gait assessment. Talocrural and subtalar cartilage was segmented manually to calculate T1ρ relaxation times. Greater T1ρ relaxation times were interpreted as decreased proteoglycan content. Pearson product–moment bivariate correlations examined the relationships between T1ρ relaxation times and the gait biomechanics and postural control outcomes.

Results
Across multiple variables, worse postural control demonstrated moderate to strong associations (range, 0.433–0.642 and −0.713) with greater talar T1ρ relaxation times. At the subtalar joint, greater T1ρ relaxation times were associated with lower peak vertical ground reaction forces, lower average vertical ground reaction force loading rates, and lower peak loading rates (range, −0.438 to −0.622). At the talar dome, greater talar T1ρ relaxation times were associated with increased knee extensor moments (r = 0.457), as well as greater knee flexion (r = 0.482) and knee adduction (r = 0.407) at initial contact. Larger step spatiotemporal gait parameters also associated with greater talar and subtalar T1ρ relaxation times (range, 0.434–0.697).

Conclusions
In individuals with CAI, worse postural control and altered kinematic, kinetic, and spatiotemporal outcomes demonstrate moderate to strong associations with greater talar T1ρ and/or subtalar relaxation times (i.e., less proteoglycan content). Associations between modifiable neuromechanical variables and greater T1ρ relaxation times may represent potential therapeutic interventions to mitigate ankle joint degeneration in those with CAI.
Sonification of weather data as a non-human-centric artistic approach
Authors: Ng Yi Kee , Lim Kok Yoong 
Published: January 2022

Background - In the mid-20th century, the emergence of sound studies demonstrated a shift of research interest in sonic practitioners. This field gains its prevalence by expanding the boundaries of prevailing conception through proposing alternative creative approaches in sound art practices. Methods – Two methods were presented – listening and sounding to promote creative sound making. The first method, listening involves soundwalking and recording sound from the external environments. These recordings were then re-evaluated and post-processed in audio editing software. The second method, sounding involves the creation of a weather data sonification system in Pure Data environment, in which the perceptual experience from the first method was taken into consideration. Result – First method enables the genesis of creative idiosyncrasies, such as preferences and ideas through the sonic perception of environmental events. In this process, noise and weather were identified as environmental components that share similar sensible qualities. Thus, noise is a prevalent medium that inspires the creation of sound generators in the sonification system presented in this paper. The sonic output of data sonification reveals an analogical connection between weather data and sonic parameters, in which changes in data values result in changes in acoustic properties. These outputs deliver different sensibilities based on their data parameters; sonification of temperature data might suggest an alarming effect to the listener. Conclusion – The proposed methods were intricately linked, suggesting environmental events to be perceived and realized through a non-scientific perspective. By highlighting aesthetic possibilities of environmental components, this paper presents an alternative perspective in contrast with the human-centric worldview through the creation of sonic works.