bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Factors of Fiducial Marker Motion and Individual Margin Assessment in Postoperative Breast Cancer Radiotherapy
Authors: Luo Jiaojiao , Peng Haiyan , Luo Huanli , Jin Fu , Wang Ying 
Published: March 2022

Abstract
Introduction: The fiducials were often used in breast cancer radiotherapy, and its motion relative to the tumor bed during treatment is nonnegligible. So, this motion should be included in the calculation of the Planning Target Volume (PTV) margins to reduce the risk of geographical miss. we assessed the correlation of fiducial motion with breast size and fiducial position, and expected to provide the reference for individualized PTV margin. Methods: Fifteen patients with radiotherapy after breast conserving surgery were included, and all signed informed consent form. The Cone Beam Computed Tomography (CBCT) was performed twice a week. The mammary basal diameter (D), the mammary height axis (H) and D times H (D×H) were measured at the slice of the tracheal bifurcation to represent the breast size. The clinical target volume (CTV) was divided into four quadrants with the tumor bed centroid as the origin. The lateromedial, anteroposterior and craniocaudal motion of fiducials were calculated (MLR, MAP, MSI), and the distance from fiducials to the innermost, foremost and uppermost of the CTV (DSLR, DSAP, DSSI) were recorded.Results: The fiducial motions tended to the right, dorsal and caudal. In the first quadrant, when D×H<99.89 cm2, the MAP was strongly correlated with D×H(r=0.805). Both MLR and MAP were positively linearly related to DSLR, DSAP, DSSI [r(R2) =0.94(0.87),0.94(0.86),0.91(0.80) And 0.87(0.76),0.91(0.80),0.92(0.83)]. The MSI was strongly linearly correlated with DSAP and DSLR, [r(R2) =0.94(0.87), 0.91(0.80)]. In the fourth quadrant, when D×H<90.71 cm2, the MAP and DSLR, the MSI and DSAP all showed strong linear negative correlations [r(R2) =-0.96(0.91), -0.95(0.87)], while the MLR and DSLR showed strong linear positive correlation [r(R2) =0.91(0.77)]. The PTV margin varied significantly between the first and fourth quadrant (p<0.05), and the largest margin was 12.4 mm in the craniocaudal direction of the first quadrant with D×H≥99.89 cm2 .Conclusion: The fiducial motion was anisotropic, and also susceptible to the breast size and fiducial position. The Individualized PTV margins should be determined based on breast size and the quadrant position of the lesion.
Sex determination through metric analysis of teeth crown
Authors: Ahn Hyo-Sang , Woo Eun Jin , Lim Joong Yeon , Kim Hee-Jin , Hu Kyung-Seok 
Published: March 2022

Abstract
Teeth that are resistant to external stimuli and have little diversity for individuals can be an important identification factor. This study aimed to present a method that can be used easily and accurately to identify individuals using their teeth, specifically to determine their sex.In this study, all teeth were extracted from 166 cadavers and, from these teeth, the crown length, mesiodistal width, and buccolingual width were measured using a digital caliper. The measurements were then analyzed using SPSS and MATLAB software.First, tooth crown morphology was compared between males and females. Regression analysis was performed for each measurement separately for both males and females. Finally, each linear discriminant was calculated from the measurements of all tooth crowns to confirm accuracy.Since teeth began to be used for individual identification, most methods required various types of teeth and measurements for sex determination, even if high accuracy was presented. Considering these limitations, sex determinants were derived from all teeth, suggesting that an easy and objective way could be derived to determine sex, even in a limited situation where all teeth could not be used.
Removal of Congo Red Using Iron-Based Nanoparticles Synthesized by Citrus Family Peel Extracts
Authors: Aydogan Tugce , Dumanli F. Tugce Senberber , Derun Emek Moroydor 
Published: March 2022

Abstract
Iron-based nanoparticles were synthesized without addition of any pH stabilizers, stabilizers and capping agent. The particles were prepared using lemon, tangerine, orange and grapefruit peel extracts. The optimum reaction parameters were determined with UV–Visible spectroscopy, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Transmittance electron microscopy (TEM) and Thermogravimetric analysis (TG/DTG) were used. XRD results identified binary formation of Fe3O4 and Fe2O3 with powder diffraction file numbers of “01-074-1910” and “03-065-0390”, respectively. According to morphological results, the smallest particle size range of 155–225 nm was observed in LP-Fe Np (iron-based nanoparticles synthesized by using lemon peel extract). UV-Vis indicated the iron nanoparticle formation in the range of 220–380 nm. The decomposition processes of the synthesized samples were occurred in multiple stages between 30 and 518°C. The smallest particles were measured at TP-Fe Np as 1.36–30.76 nm in TEM analyses. The adsorption of Congo red on the synthesized nanoparticles were also determined. The highest removal rate was obtained for the GP-Fe Np as 36.5%.
Recommendations for weight management in osteoarthritis: a systematic review of clinical practice guidelines
Authors: Lim Yuan Z. , Wong Jeffrey , Hussain Sultana Monira , Estee Mahnuma Mahfuz , Zolio Luigi , Page Matthew J. , Harrison Cheryce L. , Wluka Anita E. , Wang Yuanyuan , Cicuttini Flavia M. 
Published: March 2022

Abstract
Background: While targeting obesity is central to osteoarthritis management, recent meta-analyses demonstrate that significant weight loss has only modest effects on symptoms, and no effect on structure. In countries such as USA, adults gain on average 0.5 to 1 kilogram per year from early to middle adulthood. Preventing weight gain is easier to achieve and sustain than weight loss and reduces knee pain. The World Health Organisation has recommended that effective management of obesity should include prevention of weight gain, weight maintenance as well as weight loss; however, it is unclear whether such recommendations appear in guidelines for osteoarthritis. Therefore, we systematically reviewed the recommendations and approaches for weight management in all current clinical practice guidelines (CPGs) for osteoarthritis. Methods: Nine databases were searched (1st January 2010 to 15th March 2022) to identify guidelines informing the non-pharmacological management of osteoarthritis. Three reviewers appraised guidelines according to the AGREE II instrument, and independently extracted data on their characteristics. One author extracted and summarised guideline recommendations on weight management. This systematic review is registered on PROSPERO (CRD42021274195). Results: Fifteen CPGs from developed and developing countries were included. Weight loss was recommended for knee (12 of 13 guidelines) and hip (10 of 11 guidelines) but not hand (0 of 4 guidelines) osteoarthritis. Combination approaches of diet and/or exercise were recommended for overweight or obese individuals in knee (8 of 12) and hip (4 of 10) osteoarthritis, with 2 guidelines specifying ≥5% weight loss. One of 15 guidelines specified strategies for weight loss and maintenance of lost weight. Two of 15 guidelines recommended controlling body weight for osteoarthritis, regardless of obesity status. There was discordance between strength of recommendation for weight loss and level of evidence in 3 of 15 guidelines. The included guidelines had a median AGREE II domain score of 78.7% (interquartile range 71.4, 87.9). Conclusion: Most CPGs for knee and hip osteoarthritis recommend weight loss to manage obesity in osteoarthritis despite evidence for modest benefit. Given weight gain is common in adults, other approaches such as preventing weight gain should be considered to improve outcomes in osteoarthritis.
Upregulated ONECUT2 impeded the efficacy of 5-FU treatment to relapsed patients and might be an emerging indicator of chemotherapy in gastric cancer
Authors: Wang Xu , Zhu Haifeng , Chen Shu , Long Weiguo 
Published: March 2022

Abstract
Background Chemotherapeutic resistance is a main obstacle for prolonging the lifetime of gastric cancer (GC) patients, so new emerging solutions still remain to be investigated. We aim to identify an effective target, ONECTU2, for chemotherapy of GC patient, particularly relapsed patients.Methods STAD patients matched with clinical features and transcriptome sequencing data from TCGA were used in this study. Gene set enrichment analyses (GSEA) were performed using 100 patients who were divided average according to ONECUT2 level. Cytotoxic assays for 5-FU and cisplatin were used to test the role of chemotherapeutic resistance of ONECUT2 in GC cells. 30 relapsed patients with previously resected tumor samples were collected to validate the efficacy of ONECUT2 in GC chemotherapy.Results ONECUT2 was a crucial regulator of Disease-Free Interval (DFI) in GC patients with reoccurrence once having chemotherapeutic treatment. Gene set enrichment analysis (GSEA) results suggest that upregulated ONECUT2 is positively correlated with the increased ability of xenobiotic drug metabolism, which might contribute to the pharmaceutical resistance in GC. Targeting ONECUT2 reduced half-maximal inhibitory concentration (IC50) of 5-FU and cisplatin in GC cells. The efficacy of 5-FU treatment was negatively correlated with ONECUT2 mRNA level of tumor samples derived from GC patients who relapsed after surgical resection.Conclusions These findings indicate that ONECUT2 displays a drug-resistant function in GC, providing a new therapeutic target for GC treatment.
Investigating the Benefits of Target Selective Treatment by Average Daily Weight Gain Against Gastrointestinal Nematodes in Morada Nova Lambs
Authors: Santos Isabella Barbosa dos , Anholeto Luís Adriano , de Sousa Gustavo Avelar , Nucci Alessandra da Silva , Gainza Yousmel Aleman , Figueiredo Amanda , Santos Leonardo Aparecido Lima dos , Minho Alessandro Pelegrine , Barioni-Junior Waldomiro , Esteves Sérgio Novita , Niciura Simone Cristina Méo , Chagas Ana Carolina de Souza 
Published: March 2022

Abstract
Gastrointestinal nematodes are the major obstacle to sheep farming, and their rational control depends on reliable parameters that guarantee both productivity and animal health. Thus, the objective of this study was to evaluate a method of targeted selective treatment (TST) of Morada Nova lambs, using the average daily weight gain (DWG). For this, 114 lambs in the rainy and 102 in the dry season were randomized into three treatments: Control (CT), Routine (RT - treated every 42 days), and Targeted Selective (TST - treated according to DWG). Packed cell volume (PCV) and eggs per gram of feces (EPG) tests were performed. Intending to investigate the resistance of parasites to anthelmintic, the EPG count reduction test (FECRT), RESISTA-Test© and molecular test (detection of a 63 bp indel in the acr-8 gene in Haemonchus contortus) were performed. For CT, RT and TST groups, the following results were obtained: mean EPG were 4665.1, 3063.5 and 3462.1 in the rainy season and 4475.1, 1341.7 and 2863.4 in the dry season, respectively; mean PCV were 32.1, 33.4 and 32.3% in the rainy season and 33.9, 36.0 and 35.1% in the dry season; mean DWG were 0.087, 0.101 and 0.094 kg in the rainy season and 0.102, 0.113 and 0.112 kg in the dry season; efficacies of levamisole in FECRT were 66.4, 24.1 and 76.4% in the rainy and 90.7, 12.4 and 64.8% in the dry season, respectively; in the RESISTA-Test© LC50 were 0.482, 1.926 and 0.117 µg.mL-1 in the rainy and 0.437, 0.851 and 0.045 µg.mL-1 in the dry season, respectively; in the molecular test, the frequencies of the homozygous resistant genotype were 57.1, 71.4 and 40.0% in the rainy season and 47.8, 55.9 and 41.9% in the dry season. In conclusion, TST allowed less resistance development through refugia maintenance, without productive losses, contributing to the sustainability of the sheep chain.
Surgical administration of indocyanine green in hepatectomy for improved bile leakage detection
Authors: Hanaki Takehiko , Goto Keisuke , Morimoto Masaki , Murakami Yuki , Matsunaga Tomoyuki , Yamamoto Manabu , Tokuyasu Naruo , Sakamoto Teruhisa , Hasegawa Toshimichi , Fujiwara Yoshiyuki 
Published: March 2022

Abstract
Purpose: To study the effect of fluorescence imaging systemically administrated indocyanine green (ICG) for preventing bile leakage (BL) from the cut surface of the liver. Detection of ICG leakage from the resected surface of the liver was used to identify BL. We compared the bilirubin levels in drainage effluent between patients in whom BL from the resected liver was observed using conventional assessment with the naked eye and those in whom ICG leakage was used as an indicator of BL.Methods: Our sample comprised 26 patients who underwent hepatectomy without ICG (conventional observation group, CO-G) and 23 hepatectomy patients who underwent intraoperative detection of BL using systemically administrated ICG (ICG observation group, IO-G). In the IO-G, we also compared the number of BL spots on the resected plane visible to the naked eye with the number of visible spots on an ICG camera.Results: Three days after surgery, the bilirubin levels in the drainage fluid of the IO-G were significantly lower than those in the CO-G (1.3 vs. 1.9 mg/dL, p = 0.019). Two cases (7.7%) of ISGLS grade B biliary fistula occurred in the CO-G, but none in the IO-G. In the IO-G, the number of BL spots detected just after hepatectomy was significantly higher using ICG camera observation than the naked eye observation (0.22 vs. 0.91 spots, p < 0.001).Conclusion: Observation with systemically administrated ICG can detect BLs with more sensitivity than conventional observation with the naked eye. This enables rapid repair and may prevent biliary fistula.
Functional Specialization and Coordination in the Amygdala-Hippocampus Circuit Support Working Memory
Authors: Li Jin , Cao Dan , Dimakopoulos Vasileios , Yu Shan , Xiao Xinyu , Imbach Lukas , Stieglitz Lennart , Sarnthein Johannes , Jiang Tianzi 
Published: March 2022

Abstract
Both the hippocampus and amygdala are involved in working memory (WM) processing. However, it is still an open question how the two structures interact to represent and maintain WM content. Here, we simultaneously recorded intracranial EEG from the amygdala and hippocampus of epilepsy patients while performing a WM task. Using a series of univariate, multivariate, and connectivity analyses, our results revealed a functional specialization of the amygdala-hippocampus circuit: The mnemonic representations in the amygdala were highly distinct but decreased from encoding to maintenance. The hippocampal representations, however, were less specific but remained stable in the absence of stimulus. Furthermore, the amygdala and hippocampus coordinated their activity during WM: The hippocampal representation was reinstated in the amygdala during maintenance, with task induced low-frequency information flow from the hippocampus to the amygdala. Importantly, the functional specificity and coordination were correlated only when they appeared in correct trials. Our study provides new evidence that successful WM processing in humans relies on specialized and coordinated functions within the amygdala-hippocampus circuit.
A Priority Based Congestion Avoidance Scheme for Healthcare Wireless Sensor Networks
Authors: Gholipour Majid , Mazloomi Neda 
Published: March 2022

Abstract
The Wireless Sensor Network (WSN) is a self-organizing network consisting of a number of sensor nodes located in the monitoring area. One of the main applications of wireless sensor network is in the field of health care and remote patient monitoring. One of the most important challenges of such networks is controlling network congestion and transmitting data in a way that improves quality of service (QoS) parameters. Thus, it increasing grid performance and reducing energy consumption. Energy consumption increases due to various reasons such as unsuccessful delivery of packets to the receiver, congestion in the network, retransmission of packets, and delay in delivery packets to the sink, low received signal strength and so on. Given the importance of some data in the field of health, congestion should be avoided and secure data transmission should be ensured. This study divides the collected data into two groups based on their intrinsic characteristics by presenting a congestion management protocol: 1) critical data 2) non-critical data. The proposed protocol provides a dynamic routing algorithm based on the TOPSIS model for non-critical data transmission. In addition, an algorithm for transmitting critical data through the shortest possible path is also provided based on support vector machines. This improves the network performance through using multi-classification that is obtained from Support Vector Machines. The simulation results indicate that the proposed method works better than other methods and leads to better performance in delay, network performance and power consumption.
First Report of Cryptosporidium spp. in Cattle from Jamaica
Authors: Morris Amanda J. , Noble Simmoy , Lindo John F. , Ong Corinne S.L. 
Published: March 2022

Abstract
Background: Cryptosporidium is a protozoan parasite associated with gastrointestinal illness in humans and a wide range of vertebrate animals. Cattle, particularly calves, are widely recognized as major reservoir hosts of zoonotic Cryptosporidium spp. Despite this, and cattle being one of the most significant economic mainstays in St. Elizabeth, Jamaica, there is no publish record on the presence of Cryptosporidium in Jamaican cattle nor their potential role as zoonotic reservoirs. Methods: In the present study, 119 fecal specimens were collected from dairy and beef cattle from 10 farms in St. Elizabeth parish. Polymerase chain reaction (PCR), amplifying the 18S rRNA gene, confirmed Cryptosporidium presence in 10 (8 %) of samples. Results: Sequence analysis of PCR products identified three C. parvum isolates (previously reported in calves) and one C. hominis isolate among calves experiencing diarrhea at the time of sample collection. Further nested PCR and sequence analysis of the 60 kDa glycoprotein (gp60) gene, identified the C. hominis IbA9G2 subtype, previously reported in a calf. Conclusions: This reports the first molecular characterization of Cryptosporidium spp. from cattle in Jamaica, and their potential role as zoonotic transmitters of the protozoan parasite. Further molecular investigation is warranted to better understand direct and indirect transmission dynamics of cryptosporidiosis between livestock and humans on the island.
The host bone marrow stem cell repopulation is crucial to the permanent tolerance in 50% liver transplantation
Authors: chen guoyong , Tang gaofeng , zhao huibo , wei sidong , guo xiaoyan , liu fanhzhou , lu Di , Guo Hui , zhou shaotang 
Published: March 2022

Abstract
The severe toxicities and side-effects derived from lifelong anti-rejection therapy following organ transplantation necessitates operational tolerance, a immunosuppression-free state in which the allograft functions well and no immunological rejections occur, no operational tolerance is in use globally. Here we introduce that operational tolerance, based on the hypertrophy to hyperplasia transition upon liver regeneration, is early acquired and permanently maintained in 50% adult rat liver allograft transplantation through the host bone marrow stem cell repopulation and 9-day cyclosporine A use. Compared with whole and partial liver transplantations as the controls, longer-term survivals (over 500 days) were observed in the tolerant hosts (p=0.001), the allograft functioned well and no acute or chronic rejections were confirmed by histology examinations. Further study revealed that the allograft was reinstituted by host-derived stem cells marked with CD34, which migrated, repopulated and differentiated after mobilization. However donor-specific hypo-response was not achieved through skin transplantation, indicating no adaptive immune activity occurrence. Our protocol is characteristic of targeting the allograft and non-immunological intervention, offering a novel avenue to operational tolerance induction which is of highly clinical relevance
Item distribution, scalability and internal consistency of the QUALIDEM quality of life assessment for patients with dementia in acute hospital settings
Authors: Lüdecke Daniel , Dichter Martin Nikolaus , Nickel Stefan , Kofahl Christopher 
Published: March 2022

Abstract
Background
Quality of life (QoL) of people with dementia (PwD) is an important indicator of quality of care. Studying the impact of acute hospital settings on PwD’s QoL requires assessment instruments that consider environmental factors. Until now, dementia-specific QoL instruments have not demonstrated their feasibility in acute hospitals. Therefore, validated instruments to study QoL-outcomes of patients with dementia in hospitals are needed.
Methods
Data stem from a study that analysed the impact of a special care concept in acute hospitals for patients with dementia on their QoL. Total sample size consisted of N = 526 patients. QoL was assessed with the QUALIDEM. This instrument consists of nine subscales that can be applied to people with mild to severe dementia (N = 344), while six of the nine subscales are applicable for people with very severe dementia (N = 182). Scalability and internal consistency were tested with Mokken scale analysis.
Results
For people with mild to severe dementia, seven out of nine subscales were scalable (0.31 ≤ H ≤ 0.75). Five of these seven subscales were also internally consistent (ρ ≥ 0.69), while two had insufficient reliability scores (ρ = 0.53 and 0.52). The remaining two (positive self-image, feeling at home) subscales had rather low scalability (H = 0.17/0.16) and reliability scores (ρ = 0.35/0.36). For people with very severe dementia, all six subscales were scalable (0.34 ≤ H ≤ 0.71). Five out of six showed acceptable internal consistency (ρ = 0.65 to 0.91). Only the item social relations had insufficient reliability (ρ = 0.55).
Conclusions
In comparison with a previous evaluation of the QUALIDEM in a long-term care setting, the application in a hospital setting leads to very similar, acceptable results for people with mild to severe dementia. For people with very severe dementia, the QUALIDEM seems to fit even better in a hospital context. Results suggest either a revision of unsatisfactory items or a general reduction to six items for the QUALIDEM, for all PwD. In general, the QUALIDEM can be recommended as instrument to assess the QoL for PwD in the context of hospital research. Additionally, an investigation of the inter-rater reliability is recommended.
Simulating androgen receptor selection in designer yeast
Authors: Yuan Yingjin , Zhang Haoran , Zhang Lu , Xu Yipeng , Chen Shaoyong , Ma Zhenyi , Yao Mingdong , Li Fangyin , Li Bo 
Published: March 2022

Abstract
We established a designer yeast assay to simulate natural androgen receptor (AR) selection using AR antagonists. Yeast HIS3 gene transactivation was associated with the ligand-induced recruitment of steroid receptor coactivator-1 (SRC-1) by AR mutants, where yeast growth in histidine-free medium was determined as the outcome. Our simple and efficient assay can enable precise one-pot screening of AR mutants.