bluuebunny/crossref_metadata_2025_split huggingface dataset:
The economics of patients undergoing periacetabular osteotomy for hip dysplasia: the financial relationship between physicians and hospitals
Authors: Alter Thomas , Fitch Ashlyn , Bailey Terhune E , Williams Joel C 
Published: August 2022

ABSTRACTPeriacetabular osteotomy (PAO) is the gold standard for treating hip dysplasia in patients with preserved articular cartilage. The aim of this study is to evaluate the financial relationship between facility and professional revenue for patients undergoing PAO for hip dysplasia and acetabular version abnormalities. All patients who underwent PAO for hip dysplasia by a single surgeon at a tertiary academic medical center between December 2016 and November 2020 were identified. Financial records for facility and professional services were reviewed and analyzed. The orthopedic charge multiplier, the dollars of facility charge created by a single dollar of orthopedic professional charge, and orthopedic net revenue multiplier, the dollars collected by the hospital for facility services generated for each dollar collected by the orthopedic surgeon, were calculated. A total of 36 patients were included in the study. The mean total charge for all patients was $144 939.35 ± $23 726.48 (range $109 002.71 to $227 290.20), and the average total revenue for all patients was $44 218.79 ± $12 352.97 (range $29 397.39 to $90,830.62). The mean orthopedic charge multiplier was 2.47 ± 1.32 (range 0.78–6.53), and the net revenue collection multiplier was 8.62 ± 10.69 (range, 1.20–57.80). The majority of charges and revenue related to care of patients undergoing PAO return to the hospital. The significant mean orthopedic charge multiplier for this procedure increases the value of the service and the surgeon to hospital profitability. This information can help shape the relationship between the hospital and the surgeon and create a firm platform to advocate for program advancement.
Volcanic Exoplanet Surfaces
Authors: Fortin Marc-Antoine , Gazel Esteban , Kaltenegger Lisa , Holycross Megan E 
Published: August 2022

ABSTRACT
Recent measurements of the reflection of the surface of a lava world provides an unprecedented opportunity to investigate different stages of rocky planet evolution. The spectral features of the surfaces of rocky exoplanets give insights into their evolution and inner workings. However, no data base exists yet that contains spectral reflectivity and emission of a wide range of potential volcanic exoplanet surface materials. Here, we first synthesized 16 possible exoplanet surfaces, spanning a wide range of chemical compositions based on potential mantle material of volcanic exoplanets guided by the metallicity of different host stars. Then, we measured their infrared reflection spectrum (2.5–28 μm), from which we obtained their emission spectra, and established the link between the composition and a strong spectral feature at 8 μm, the Christiansen feature (CF). Our analysis suggests a new multicomponent composition relationship with the CF as well as a correlation with the silica content of the exoplanet mantle. We also report the mineralogies of these materials, as possibilities for volcanic worlds. This data base is a tool to aid in the interpretation of future spectra of volcanic and lava worlds that will be collected by the James Webb Space Telescope and other missions.
Interstellar extinction correction in ionized regions using He i lines
Authors: Zamora S , Díaz Ángeles I , Terlevich Elena , Fernández Vital 
Published: August 2022

ABSTRACT
The logarithmic extinction coefficient, c(H β), is usually derived using the H α/H β ratio for case B recombination and assuming standard values of electron density and temperature. However, the use of strong Balmer lines can lead us to selection biases when studying regions with different surface brightness, such as extended nebulae, with the use of single integral field spectroscopy observations, since, in some cases, the H α line can be saturated in moderate to long exposures. In this work, we present a method to derive extinction corrections based only on the weaker lines of He i, taking into account the presence of triplet states in these atoms and its influence on recombination lines. We have applied this procedure to calculate the extinction of different regions of the 30 Doradus nebula from MUSE integral-field spectroscopy data. The comparison between helium and hydrogen c(H β) determinations has been found to yield results fully compatible within the errors and the use of both sets of lines simultaneously reduces considerably the error in the derivation.
How baryons affect haloes and large-scale structure: a unified picture from the Simba simulation
Authors: Sorini Daniele , Davé Romeel , Cui Weiguang , Appleby Sarah 
Published: August 2022

ABSTRACT
Using the state-of-the-art suite of hydrodynamic simulations Simba, as well as its dark-matter-only counterpart, we study the impact of the presence of baryons and of different stellar/AGN feedback mechanisms on large-scale structure, halo density profiles, and on the abundance of different baryonic phases within haloes and in the intergalactic medium (IGM). The unified picture that emerges from our analysis is that the main physical drivers shaping the distribution of matter at all scales are star formation-driven galactic outflows at z &gt; 2 for lower mass haloes and AGN jets at z &lt; 2 in higher mass haloes. Feedback suppresses the baryon mass function with time relative to the halo mass function, and it even impacts the halo mass function itself at the ∼20 per cent level, particularly evacuating the centres and enhancing dark matter just outside haloes. At early epochs baryons pile up in the centres of haloes, but by late epochs and particularly in massive systems gas has mostly been evacuated from within the inner halo. AGN jets are so efficient at such evacuation that at low redshifts the baryon fraction within ∼1012–1013 M⊙ haloes is only 25 per cent of the cosmic baryon fraction, mostly in stars. The baryon fraction enclosed in a sphere around such haloes approaches the cosmic value Ωb/Ωm only at 10–20 virial radii. As a result, 87 per cent of the baryonic mass in the Universe lies in the IGM at z = 0, with 67 per cent being in the form of warm-hot IGM (T &gt; 105K).
A TESS search for donor-star pulsations in high-mass X-ray binaries
Authors: Ramsay Gavin , Hakala Pasi , Charles Philip A 
Published: August 2022

ABSTRACT
Ground-based optical photometry of the counterparts of high-mass X-ray binaries (HMXBs) has revealed the presence of periodic modulations on time-scales of ∼0.3–0.5 d. More recent space-based observations (CoRoT and TESS) of OB and Be stars have shown that pulsations caused by p and g modes are common in early-type stars. We have therefore undertaken a systematic search for variability in the optical counterparts of 23 HMXBs (mostly neutron star systems, but including one black hole, Cyg X-1) using TESS data primarily in 2 min cadence mode. After removing the orbital period modulation in four systems, we find that all 23 sources show evidence for quasi-periodic variability on periods shorter than ∼1 d. We compare their power spectra with those from observations of other OB- and Be-type stars. In two systems, V725 Tau and HD 249179 (which may not be an HMXB), we find evidence for an outburst, the former being simultaneous with an X-ray flare. We search for changes in the power spectra over the outburst duration and compare them with outbursts seen in other Be systems.
Mammalian RNase H1 directs RNA primer formation for mtDNA replication initiation and is also necessary for mtDNA replication completion
Authors: Misic Jelena , Milenkovic Dusanka , Al-Behadili Ali , Xie Xie , Jiang Min , Jiang Shan , Filograna Roberta , Koolmeister Camilla , Siira Stefan J , Jenninger Louise , Filipovska Aleksandra , Clausen Anders R , Caporali Leonardo , Valentino Maria Lucia , La Morgia Chiara , Carelli Valerio , Nicholls Thomas J , Wredenberg Anna , Falkenberg Maria , Larsson Nils-Göran 
Published: August 2022

Abstract
The in vivo role for RNase H1 in mammalian mitochondria has been much debated. Loss of RNase H1 is embryonic lethal and to further study its role in mtDNA expression we characterized a conditional knockout of Rnaseh1 in mouse heart. We report that RNase H1 is essential for processing of RNA primers to allow site-specific initiation of mtDNA replication. Without RNase H1, the RNA:DNA hybrids at the replication origins are not processed and mtDNA replication is initiated at non-canonical sites and becomes impaired. Importantly, RNase H1 is also needed for replication completion and in its absence linear deleted mtDNA molecules extending between the two origins of mtDNA replication are formed accompanied by mtDNA depletion. The steady-state levels of mitochondrial transcripts follow the levels of mtDNA, and RNA processing is not altered in the absence of RNase H1. Finally, we report the first patient with a homozygous pathogenic mutation in the hybrid-binding domain of RNase H1 causing impaired mtDNA replication. In contrast to catalytically inactive variants of RNase H1, this mutant version has enhanced enzyme activity but shows impaired primer formation. This finding shows that the RNase H1 activity must be strictly controlled to allow proper regulation of mtDNA replication.
Resistance of mitochondrial DNA to cadmium and Aflatoxin B1 damage-induced germline mutation accumulation inC. elegans
Authors: Leuthner Tess C , Benzing Laura , Kohrn Brendan F , Bergemann Christina M , Hipp Michael J , Hershberger Kathleen A , Mello Danielle F , Sokolskyi Tymofii , Stevenson Kevin , Merutka Ilaria R , Seay Sarah A , Gregory Simon G , Kennedy Scott R , Meyer Joel N 
Published: August 2022

AbstractMitochondrial DNA (mtDNA) is prone to mutation in aging and over evolutionary time, yet the processes that regulate the accumulation of de novo mtDNA mutations and modulate mtDNA heteroplasmy are not fully elucidated. Mitochondria lack certain DNA repair processes, which could contribute to polymerase error-induced mutations and increase susceptibility to chemical-induced mtDNA mutagenesis. We conducted error-corrected, ultra-sensitive Duplex Sequencing to investigate the effects of two known nuclear genome mutagens, cadmium and Aflatoxin B1, on germline mtDNA mutagenesis in Caenorhabditis elegans. Detection of thousands of mtDNA mutations revealed pervasive heteroplasmy in C. elegans and that mtDNA mutagenesis is dominated by C:G → A:T mutations generally attributed to oxidative damage. However, there was no effect of either exposure on mtDNA mutation frequency, spectrum, or trinucleotide context signature despite a significant increase in nuclear mutation rate after aflatoxin B1 exposure. Mitophagy-deficient mutants pink-1 and dct-1 accumulated significantly higher levels of mtDNA damage compared to wild-type C. elegans after exposures. However, there were only small differences in mtDNA mutation frequency, spectrum, or trinucleotide context signature compared to wild-type after 3050 generations, across all treatments. These findings suggest mitochondria harbor additional previously uncharacterized mechanisms that regulate mtDNA mutational processes across generations.
SUMMER: a Mendelian randomization interactive server to systematically evaluate the causal effects of risk factors and circulating biomarkers on pan-cancer survival
Authors: Xin Junyi , Gu Dongying , Chen Silu , Ben Shuai , Li Huiqin , Zhang Zhengdong , Du Mulong , Wang Meilin 
Published: August 2022

Abstract
Genome-wide association studies (GWASs) underlying case-control design have uncovered hundreds of genetic loci involved in tumorigenesis and provided rich resources for identifying risk factors and biomarkers associated with cancer susceptibility. However, the application of GWAS in determining the genetic architecture of cancer survival remains unestablished. Here, we systematically evaluated genetic effects at the genome-wide level on cancer survival that included overall survival (OS) and cancer-specific survival (CSS), leveraging data deposited in the UK Biobank cohort of a total of 19 628 incident patients across 17 cancer types. Furthermore, we assessed the causal effects of risk factors and circulating biomarkers on cancer prognosis via a Mendelian randomization (MR) analytic framework, which integrated cancer survival GWAS dataset, along with phenome-wide association study (PheWAS) and blood genome-wide gene expression/DNA methylation quantitative trait loci (eQTL/meQTL) datasets. On average, more than 10 traits, 700 genes, and 4,500 CpG sites were prone to cancer prognosis. Finally, we developed a user-friendly online database, SUrvival related cancer Multi-omics database via MEndelian Randomization (SUMMER; http://njmu-edu.cn:3838/SUMMER/), to help users query, browse, and download cancer survival results. In conclusion, SUMMER provides an important resource to assist the research community in understanding the genetic mechanisms of cancer survival.
Differential dependencies of human RNA polymerase II promoters on TBP, TAF1, TFIIB and XPB
Authors: Santana Juan F , Collins Geoffrey S , Parida Mrutyunjaya , Luse Donal S , Price David H 
Published: August 2022

Abstract
The effects of rapid acute depletion of components of RNA polymerase II (Pol II) general transcription factors (GTFs) that are thought to be critical for formation of preinitiation complexes (PICs) and initiation in vitro were quantified in HAP1 cells using precision nuclear run-on sequencing (PRO-Seq). The average dependencies for each factor across &gt;70 000 promoters varied widely even though levels of depletions were similar. Some of the effects could be attributed to the presence or absence of core promoter elements such as the upstream TBP-specificity motif or downstream G-rich sequences, but some dependencies anti-correlated with such sequences. While depletion of TBP had a large effect on most Pol III promoters only a small fraction of Pol II promoters were similarly affected. TFIIB depletion had the largest general effect on Pol II and also correlated with apparent termination defects downstream of genes. Our results demonstrate that promoter activity is combinatorially influenced by recruitment of TFIID and sequence-specific transcription factors. They also suggest that interaction of the preinitiation complex (PIC) with nucleosomes can affect activity and that recruitment of TFIID containing TBP only plays a positive role at a subset of promoters.
Urinary extracellular vesicles and tubular transport
Authors: Rudolphi Crissy F , Blijdorp Charles J , van Willigenburg Hester , Salih Mahdi , Hoorn Ewout J 
Published: August 2022

ABSTRACT
Tubular transport is a key function of the kidney to maintain electrolyte and acid-base homeostasis. Urinary extracellular vesicles (uEVs) harbor water, electrolyte, and acid-base transporters expressed at the apical plasma membrane of tubular epithelial cells. Within the uEV proteome, the correlations between kidney and uEV protein abundances are strongest for tubular transporters. Therefore, uEVs offer a noninvasive approach to probing tubular transport in health and disease. Here, we review how kidney tubular physiology is reflected in uEVs and, conversely, how uEVs may modify tubular transport. Clinically, uEV tubular transporter profiling has been applied to rare diseases, such as inherited tubulopathies, but also to more common conditions, such as hypertension and kidney disease. Although uEVs hold the promise to advance the diagnosis of kidney disease to the molecular level, several biological and technical complexities must still be addressed. The future will tell whether uEV analysis will mainly be a powerful tool to study tubular physiology in humans or whether it will move forward to become a diagnostic bedside test.
#Curbsiding: Potential Value and Patient Confidentiality Implications of Infectious Disease Clinician Peer Consultations via Social Media
Authors: Boyland Ryan , Marcelin Jasmine R , Cortés-Penfield Nicolás 
Published: August 2022

Abstract

Background
Infectious Disease (ID) clinician’s social media use for peer consultation is unstudied.

Methods
We reviewed ID peer consultation via Twitter over a 6-week period.

Results
We found this practice frequently solicited meaningful replies, but we identified potential for confidentiality breaches.

Conclusions
We offer recommendations for responsible discussion of clinical scenarios via social media.
Phloem Unloading in Developing Rice Caryopses and its Contribution to Non-Structural Carbohydrate Translocation from Stems and Grain Yield Formation
Authors: Li Guohui , Cui Kehui , Hu Qiuqian , Wang Wencheng , Pan Junfeng , Zhang Guo , Shi Yange , Nie Lixiao , Huang Jianliang , Peng Shaobing 
Published: August 2022

Abstract
Phloem unloading plays an important role in photoassimilate partitioning and grain yield improvements in cereal crops. The phloem unloading strategy and its effects on photoassimilate translocation and yield formation remain unclear in rice. In this study, plasmodesmata were observed at the interface between the sieve elements (SEs) and companion cells (CCs), and between the SE–CC complex and surrounding parenchyma cells (PCs) in phloem of the dorsal vascular bundle in developing caryopses. Carboxyfluorescein (CF) signal was detected in the phloem of caryopses, which showed that CF was unloaded into caryopses. These results indicated that the SE–CC complex was symplasmically connected with adjacent PCs by plasmodesmata. Gene expression for sucrose transporter (SUT) and cell wall invertase (CWI), and OsSUT1 and OsCIN1 proteins were detected in developing caryopses, indicating that rice plants might actively unload sucrose into caryopses by the apoplasmic pathway. Among three rice recombinant inbred lines, R201 exhibited lower plasmodesmal densities at the boundaries between cell types (SE–CC, SE–PC and CC–PC) in developing caryopses than R91 and R156. R201 also had lower expression of SUT and CWI genes and lower protein levels of OsSUT1 and OsCIN1, as well as CWI activity, than R91 and R156. These data agreed with stem non-structural carbohydrate (NSC) translocation and grain yields for the three lines. The nitrogen application rate had no significant effect on plasmodesmal densities at the interfaces between different cells types, and did not affect CF unloading in the phloem of developing caryopses. Low nitrogen treatment enhanced expression levels of OsSUT and OsCIN genes in the three lines. These results suggested that nitrogen application had no substantial effect on symplasmic unloading but affected apoplasmic unloading. Therefore, we concluded that poor symplasmic and apoplasmic unloading in developing caryopses might result in low stem NSC translocation and poor grain yield formation of R201.
Ascorbate peroxidase postcold regulation of chloroplast NADPH dehydrogenase activity controls cold memory
Authors: Seiml-Buchinger Victoria , Reifschneider Elena , Bittner Andras , Baier Margarete 
Published: August 2022

AbstractExposure of Arabidopsis (Arabidopsis thaliana) to 4°C imprints a cold memory that modulates gene expression in response to a second (triggering) stress stimulus applied several days later. Comparison of plastid transcriptomes of cold-primed and control plants directly before they were exposed to the triggering stimulus showed downregulation of several subunits of chloroplast NADPH dehydrogenase (NDH) and regulatory subunits of ATP synthase. NDH is, like proton gradient 5 (PGR5)–PGR5-like1 (PGRL1), a thylakoid-embedded, ferredoxin-dependent plastoquinone reductase that protects photosystem I and stabilizes ATP synthesis by cyclic electron transport (CET). Like PGRL1A and PGRL1B transcript levels, ndhA and ndhD transcript levels decreased during the 24-h long priming cold treatment. PGRL1 transcript levels were quickly reset in the postcold phase, but expression of ndhA remained low. The transcript abundances of other ndh genes decreased within the next days. Comparison of thylakoid-bound ascorbate peroxidase (tAPX)-free and transiently tAPX-overexpressing or tAPX-downregulating Arabidopsis lines demonstrated that ndh expression is suppressed by postcold induction of tAPX. Four days after cold priming, when tAPX protein accumulation was maximal, NDH activity was almost fully lost. Lack of the NdhH-folding chaperonin Crr27 (Cpn60β4), but not lack of the NDH activity modulating subunits NdhM, NdhO, or photosynthetic NDH subcomplex B2 (PnsB2), strengthened priming regulation of zinc finger of A. thaliana 10, which is a nuclear-localized target gene of the tAPX-dependent cold-priming pathway. We conclude that cold-priming modifies chloroplast-to-nucleus stress signaling by tAPX-mediated suppression of NDH-dependent CET and that plastid-encoded NdhH, which controls subcomplex A assembly, is of special importance for memory stabilization.
Considerations for Lumbar Medial Branch Nerve Radiofrequency at Spinal Motion Segments Adjacent to a Fusion Construct
Authors: Turtle Joel , Miller Scott , Yang Aaron , Hurley Robert W , Spina Nicholas , McCormick Zachary L 
Published: August 2022

Abstract
Instrumented lumbar spinal fusion is common and results in biomechanical changes at adjacent spinal segments that increase facet load bearing. This can cause facet-mediated pain at levels adjacent to the surgical construct. Medial branch nerve radiofrequency ablation (RFA) exists as a treatment for some cases. It is important to acknowledge that the approach and instrumentation used during some specific lumbar fusion approaches will disrupt the medial branch nerve(s). Thus, the proceduralist must consider the fusion approach when determining which medial branch nerves are necessary to anesthetize for diagnosis and then to potentially target with RFA. This article discusses the relevant technical considerations for preparing for RFA to denervate lumbosacral facet joints adjacent to fusion constructs.
C25-modified rifamycin derivatives with improved activity against Mycobacterium abscessus
Authors: Paulowski Laura , Beckham Katherine S H , Johansen Matt D , Berneking Laura , Van Nhi , Degefu Yonatan , Staack Sonja , Sotomayor Flor Vasquez , Asar Lucia , Rohde Holger , Aldridge Bree B , Aepfelbacher Martin , Parret Annabel , Wilmanns Matthias , Kremer Laurent , Combrink Keith , Maurer Florian P 
Published: August 2022

Abstract
Infections caused by Mycobacterium abscessus are difficult to treat due to its intrinsic resistance to most antibiotics. Formation of biofilms and the capacity of M. abscessus to survive inside host phagocytes further complicate eradication. Herein, we explored whether addition of a carbamate-linked group at the C25 position of rifamycin SV blocks enzymatic inactivation by ArrMab, an ADP-ribosyltransferase conferring resistance to rifampicin (RMP). Unlike RMP, 5j, a benzyl piperidine rifamycin derivative with a morpholino substituted C3 position and a naphthoquinone core, is not modified by purified ArrMab. Additionally, we show that the ArrMab D82 residue is essential for catalytic activity. Thermal profiling of ArrMab in the presence of 5j, RMP, or rifabutin shows that 5j does not bind to ArrMab. We found that the activity of 5j is comparable to amikacin against M. abscessus planktonic cultures and pellicles. Critically, 5j also exerts potent antimicrobial activity against M. abscessus in human macrophages and shows synergistic activity with amikacin and azithromycin.