bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-Saccharomyces yeast for lowering wine alcohol levels: partial aeration versus standard conditions
Authors: Jolly Neil , Mehlomakulu Ngwekazi N , Nortje Stephan , Beukes Louisa , Hoff Justin , Booyse Mardé , Erten Hüseyin 
Published: February 2022

ABSTRACT
Non-Saccharomyces yeasts have been suggested for use in wine production for lowering alcohol content. In this study, 23 non-Saccharomyces yeasts were investigated in laboratory-scale trials using previously frozen grape must. Both aerated and standard fermentation conditions were investigated and the fermentations were co-inoculated with a commercial Saccharomyces cerevisiae reference yeast strain. Sugar consumed for percentage alcohol formed was calculated from sugar and alcohol measurements. The non-Saccharomyces yeasts showed greater variability in sugar consumption compared with the S. cerevisiae reference yeast. Two of the yeast strains (Starmerella bacillaris and Wickerhamomyces anomalus) consumed more sugar than the S. cerevisiae reference yeast under the same conditions. These two strains were subsequently used in a small-scale wine production trial following a similar aeration and standard fermentation strategy. The wine production trials using aeration compared with the standard strategy showed shorter fermentation times, increased biomass formation and more sugar utilized for alcohol produced, but reduced wine quality. The same yeasts under standard fermentation conditions also showed increased use of sugar, but neutral or positive effects on wine quality. The S. bacillaris strain showed the most potential for use in wine production for lowering alcohol content.
Staying healthy during COVID-19 crisis: well-being and salutogenic crafting among German and Swiss working population
Authors: Brauchli Rebecca , Kerksieck Philipp , Tušl Martin , Bauer Georg F 
Published: February 2022

Summary
The COVID-19 pandemic induced considerable changes regarding our working and private lives. This study aimed to examine the psychosocial effects of the COVID-19 crisis on German and Swiss employees. We analyzed the impact of the crisis on working and private life, well-being and health indicators. We tried to understand how the salutogenic behavior of crafting helps to overcome adversities during the COVID-19 pandemic and to maintain well-being and health. Therefore, we conducted a follow-up online survey from 9 to 22 April 2020 among 597 employees that had participated in the first wave of the survey in June 2019. This follow-up study design offered the opportunity to compare the situation of survey participants before and after the COVID-19 outbreak. This pre–post comparison was possible through the design of our study, which allowed us to link participants in an individual, yet anonymized way from t0 to t1. Results of the study showed that the situation concerning psychosocial factors at work and in private life and several well-being and health indicators was stable or even improved. Many study variables even remarkably improved among high crafters—a group of employees who tend to regularly craft their job and private life. Our findings indicate that employees are coping with the crisis surprisingly well. Moreover, there seem to exist beneficial, salutogenic behaviors (i.e. crafting) that allow people to better cope with crises such as the COVID-19 pandemic. These behaviors should be induced and promoted by interventions as they could be especially beneficial for low crafters.
Cytomegalovirus Latent Infection is Associated with an Increased Risk of COVID-19-Related Hospitalization
Authors: Alanio Cécile , Verma Anurag , Mathew Divij , Gouma Sigrid , Liang Guanxiang , Dunn Thomas , Oldridge Derek A , Weaver JoEllen , Kuri-Cervantes Leticia , Pampena M Betina , Betts Michael R , Collman Ronald G , Bushman Frederic D , Meyer Nuala J , Hensley Scott E , Rader Daniel , Wherry E John ,   The UPenn COVID Processing Unit, Baxter Amy E , D’Andrea Kurt , Adamski Sharon , Alam Zahidul , Addison Mary M , Byrne Katelyn T , Chandra Aditi , Descamps Hélène C , Han Nicholas , Kaminskiy Yaroslav , Kammerman Shane C , Kim Justin , Greenplate Allison R , Hamilton Jacob T , Markosyan Nune , Noll Julia Han , Omran Dalia K , Pattekar Ajinkya , Perkey Eric , Prager Elizabeth M , Pueschl Dana , Rennels Austin , Shah Jennifer B , Shilan Jake S , Wilhausen Nils , Vanderbeck Ashley N 
Published: February 2022

Abstract
Some risk factors for severe coronavirus disease 2019 (COVID-19) have been identified, including age, race, and obesity. However, 20%–50% of severe cases occur in the absence of these factors. Cytomegalovirus (CMV) is a herpesvirus that infects about 50% of all individuals worldwide and is among the most significant nongenetic determinants of immune system. We hypothesized that latent CMV infection might influence the severity of COVID-19. Our analyses demonstrate that CMV seropositivity is associated with more than twice the risk of hospitalization due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Immune profiling of blood and CMV DNA quantitative polymerase chain reaction in a subset of patients for whom respiratory tract samples were available revealed altered T-cell activation profiles in absence of extensive CMV replication in the upper respiratory tract. These data suggest a potential role for CMV-driven immune perturbations in affecting the outcome of SARS-CoV-2 infection and may have implications for the discrepancies in COVID-19 severity between different human populations.
Tofacitinib Treatment in Primary Herpes Simplex Encephalitis Interferes With Antiviral Response
Authors: Krzyzowska Malgorzata , Jarneborn Anders , Thorn Karolina , Eriksson Kristina , Jin Tao 
Published: February 2022

Abstract
Tofacitinib, a Janus kinase inhibitor, is a novel immunosuppressive drug for treatment of rheumatoid arthritis. Herpes simplex virus type 1 (HSV-1) may cause encephalitis during primary infection or following reactivation from a latent state. Long-term tofacitinib treatment may increase the risk of this life-threatening condition. The aim of this study was to investigate the effect of tofacitinib on HSV-1 primary infection using a mouse model. Mice pretreated with tofacitinib were intranasally infected with a clinical strain of HSV-1 and monitored for infection severity and antiviral response. Tofacitinib treatment of HSV-1 primary infection resulted in increased viral loads and worsened clinical outcome. Furthermore, tofacitinib promoted M2 anti-inflammatory phenotype of microglia and infiltrating monocytes, as well as inhibited production of inflammatory and antiviral cytokines by macrophages in vitro. Our findings show that treatment with tofacitinib increases severity of herpes simplex encephalitis in mice, by impairing antiviral response induced by monocytes and microglia.
Disseminated Mycobacterium abscessus Infection in a Burn Patient
Authors: Hsu Emily H , Young Sierra , Clayton Nicola , Lee Jin , Hauser Naomi , Penn Bennett , Sen Soman 
Published: February 2022

Abstract
Disseminated infection caused by nontuberculous mycobacteria (NTM) is very rare, with an incidence of 1.0 to 1.8 cases per 100,000 persons, and typically only occurs in severely immunocompromised hosts. Burn patients suffer a loss of the natural cutaneous barrier as well as injury-induced immune dysfunction, and as a result, commonly develop infections, especially with multidrug-resistant organisms. However, very few NTM infections in burn patients have been reported in the literature. Disseminated NTM infection, in particular, can be a challenge to diagnose in burn patients due to burn-related physiology such as hyperpyrexia and widespread skin injury. We present a case of disseminated infection leading to bacteremia caused by Mycobacterium abscessus in a critically ill burn patient with a 74% total body surface area burn. M. abscessus belongs to the subgroup of NTM known as rapidly growing mycobacteria, which are notable for their ability to form colonies in a matter of days, rather than weeks, and because they are often highly drug-resistant, which complicates antimicrobial therapy. This is the third reported case of bacteremia caused by NTM in a burn patient and the second case that was successfully transitioned from intravenous antimicrobials to an oral regimen.
Adaptive evolution of Methylotuvimicrobium alcaliphilum to grow in the presence of rhamnolipids improves fatty acid and rhamnolipid production from CH4
Authors: Awasthi Deepika , Tang Yung-Hsu , Amer Bashar , Baidoo Edward E K , Gin Jennifer , Chen Yan , Petzold Christopher J , Kalyuzhnaya Marina , Singer Steven W 
Published: February 2022

Abstract
Rhamnolipids (RLs) are well-studied biosurfactants naturally produced by pathogenic strains of Pseudomonas aeruginosa. Current methods to produce RLs in native and heterologous hosts have focused on carbohydrates as production substrate; however, methane (CH4) provides an intriguing alternative as a substrate for RL production because it is low cost and may mitigate greenhouse gas emissions. Here, we demonstrate RL production from CH4 by Methylotuvimicrobium alcaliphilum DSM19304. RLs are inhibitory to M. alcaliphilum growth (&lt;0.05 g/l). Adaptive laboratory evolution was performed by growing M. alcaliphilum in increasing concentrations of RLs, producing a strain that grew in the presence of 5 g/l of RLs. Metabolomics and proteomics of the adapted strain grown on CH4 in the absence of RLs revealed metabolic changes, increase in fatty acid production and secretion, alterations in gluconeogenesis, and increased secretion of lactate and osmolyte products compared with the parent strain. Expression of plasmid-borne RL production genes in the parent M. alcaliphilum strain resulted in cessation of growth and cell death. In contrast, the adapted strain transformed with the RL production genes showed no growth inhibition and produced up to 1 μM of RLs, a 600-fold increase compared with the parent strain, solely from CH4. This work has promise for developing technologies to produce fatty acid-derived bioproducts, including biosurfactants, from CH4.
Photosynthetic acclimation and sensitivity to short- and long-term environmental changes in a drought-prone forest
Authors: Schönbeck Leonie , Grossiord Charlotte , Gessler Arthur , Gisler Jonas , Meusburger Katrin , D’Odorico Petra , Rigling Andreas , Salmon Yann , Stocker Benjamin D , Zweifel Roman , Schaub Marcus 
Published: February 2022

Abstract
Future climate will be characterized by an increase in frequency and duration of drought and warming that exacerbates atmospheric evaporative demand. How trees acclimate to long-term soil moisture changes and whether these long-term changes alter trees’ sensitivity to short-term (day to months) variations of vapor pressure deficit (VPD) and soil moisture is largely unknown. Leaf gas exchange measurements were performed within a long-term (17 years) irrigation experiment in a drought-prone Scots pine-dominated forest in one of Switzerland’s driest areas on trees in naturally dry (control), irrigated, and ‘irrigation-stop’ (after 11 years of irrigation) conditions. Seventeen years of irrigation increased photosynthesis (A) and stomatal conductance (gs) and reduced gs sensitivity to increasing VPD and soil drying. Following irrigation-stop, gas exchange decreased only after 3 years. After 5 years, maximum carboxylation (Vcmax) and electron transport (Jmax) rates in irrigation-stop recovered to similar levels as to before the irrigation-stop. These results suggest that long-term release from soil drought reduces the sensitivity to VPD and that atmospheric constraints may play an increasingly important role in combination with soil drought. Moreover, our study indicates that structural adjustments lead to an attenuation of initially strong leaf-level acclimation to strong multiple-year drought.
Expression of flavodiiron protein rescues defects in electron transport around PSI resulting from overproduction of Rubisco activase in rice
Authors: Suganami Mao , Konno So , Maruhashi Ryo , Takagi Daisuke , Tazoe Youshi , Wada Shinya , Yamamoto Hiroshi , Shikanai Toshiharu , Ishida Hiroyuki , Suzuki Yuji , Makino Amane 
Published: February 2022

AbstractFragility of photosystem I has been observed in transgenic rice plants that overproduce Rubisco activase (RCA). In this study, we examined the effects of RCA overproduction on the sensitivity of PSI to photoinhibition in three lines of plants overexpressing RCA (RCA-ox). In all the RCA-ox plants the quantum yield of PSI [Y(I)] decreased whilst in contrast the quantum yield of acceptor-side limitation of PSI [Y(NA)] increased, especially under low light conditions. In the transgenic line with the highest RCA content (RCA-ox 1), the quantum yield of PSII [Y(II)] and CO2 assimilation also decreased under low light. When leaves were exposed to high light (2000 μmol photon m−2 s−1) for 60 min, the maximal activity of PSI (Pm) drastically decreased in RCA-ox 1. These results suggested that overproduction of RCA disturbs PSI electron transport control, thus increasing the susceptibility of PSI to photoinhibition. When flavodiiron protein (FLV), which functions as a large electron sink downstream of PSI, was expressed in the RCA-ox 1 background (RCA-FLV), PSI and PSII parameters, and CO2 assimilation were recovered to wild-type levels. Thus, expression of FLV restored the robustness of PSI in RCA-ox plants.
Wheat photosystem II heat tolerance responds dynamically to short- and long-term warming
Authors: Posch Bradley C , Hammer Julia , Atkin Owen K , Bramley Helen , Ruan Yong-Ling , Trethowan Richard , Coast Onoriode 
Published: February 2022

Abstract
Wheat photosynthetic heat tolerance can be characterized using minimal chlorophyll fluorescence to quantify the critical temperature (Tcrit) above which incipient damage to the photosynthetic machinery occurs. We investigated intraspecies variation and plasticity of wheat Tcrit under elevated temperature in field and controlled-environment experiments, and assessed whether intraspecies variation mirrored interspecific patterns of global heat tolerance. In the field, wheat Tcrit varied diurnally—declining from noon through to sunrise—and increased with phenological development. Under controlled conditions, heat stress (36 °C) drove a rapid (within 2 h) rise in Tcrit that peaked after 3–4 d. The peak in Tcrit indicated an upper limit to PSII heat tolerance. A global dataset [comprising 183 Triticum and wild wheat (Aegilops) species] generated from the current study and a systematic literature review showed that wheat leaf Tcrit varied by up to 20 °C (roughly two-thirds of reported global plant interspecies variation). However, unlike global patterns of interspecies Tcrit variation that have been linked to latitude of genotype origin, intraspecific variation in wheat Tcrit was unrelated to that. Overall, the observed genotypic variation and plasticity of wheat Tcrit suggest that this trait could be useful in high-throughput phenotyping of wheat photosynthetic heat tolerance.
Overexpression of SHORT-ROOT2 transcription factor enhances the outgrowth of mature axillary buds in poplar trees
Authors: Yi Minglei , Yang Heyu , Yang Shaohui , Wang Jiehua 
Published: February 2022

Abstract
SHORT-ROOT (SHR) transcription factors play important roles in asymmetric cell division and radial patterning of Arabidopsis roots. In hybrid poplar (P. tremula × P. alba clone INRA 717-1B4), PtaSHR2 was preferentially expressed in axillary buds (AXBs) and transcriptionally up-regulated during AXB maturation and activation. Overexpression of SHR2 (PtSHR2OE) induced an enhanced outgrowth of AXBs below the bud maturation point, with a simultaneous transition of an active shoot apex into an arrested terminal bud. The larger and more mature AXBs of PtSHR2OE trees revealed altered expression of genes involved in axillary meristem initiation and bud activation, as well as a higher ratio of cytokinin to auxin. To elucidate the underlying mechanism of PtSHR2OE-induced high branching, subsequent molecular and biochemical studies showed that compared with wild-type trees, decapitation induced a quicker bud outburst in PtSHR2OE trees, which could be fully inhibited by exogenous application of auxin or cytokinin biosynthesis inhibitor, but not by N-1-naphthylphthalamic acid. Our results indicated that overexpression of PtSHR2B disturbed the internal hormonal balance in AXBs by interfering with the basipetal transport of auxin, rather than causing auxin biosynthesis deficiency or auxin insensitivity, thereby releasing mature AXBs from apical dominance and promoting their outgrowth.
Transgene-free genome editing supports CCD4 role as a negative regulator of β-carotene in banana
Authors: Awasthi Praveen , Khan Shahirina , Lakhani Hiralben , Chaturvedi Siddhant , Shivani  , Kaur Navneet , Singh Jagdeep , Kesarwani Atul Kumar , Tiwari Siddharth 
Published: February 2022

Abstract
This study aims to understand the regulatory mechanism of the β-carotene homeostasis by establishing transgene-free genome editing in banana. Carotenoid cleavage dioxygenases (CCDs) belong to a miniature gene family having an imperative role in the intricated carotenoid metabolism in plants. Here, the expression pattern of multiple CCDs was correlated with the levels of carotenoid accumulation in two contrasting cultivars, viz., Nendran (high β-carotene) and Rasthali (low β-carotene). The higher expression of the RAS-CCD4 inversely correlated with β-carotene accumulation in fruit-pulp of the Rasthali. The docking analysis followed enzyme assay of purified RAS-CCD4 suggested β-carotene and 10-apo-β-carotenal as its preferred substrates. Bacterial complementation assay affirmed RAS-CCD4 role in β-carotene degradation and then overexpression of the RAS-CCD4 in the Arabidopsis thaliana further validated results in-vivo by the significant reduction in β-carotene. Subsequently, CRISPR/Cas9 mediated editing of CCD4 was demonstrated in the protoplasts and embryogenic cell lines of Rasthali. The carotenoid profiling in stable mutant lines revealed higher fold β-carotene accumulation in non-green tissue (roots) than in green tissue (leaf) compared with the unedited control plants. The differential expression of carotenoid pathway genes was correlated with the metabolites in the edited lines. The study suggests that carotenoid catabolism is regulated by the CCD4 in tissue and cultivar specific manners, and also demonstrated the use of the genome editing tool in developing transgene-free biofortified banana.
Custom methods to identify conserved genetic modules applied to novel transcriptomic data from Amborella trichopoda
Authors: Rivarola Sena Ana C , Andres-Robin Amélie , Vialette Aurelie C , Just Jérémy , Launay-Avon Alexandra , Borrega Néro , Dubreucq Bertrand , Scutt Charles P 
Published: February 2022

Abstract
We have devised a procedure for the inter-species comparison of transcriptomic data and used this procedure to reconstruct the expression dynamics of major genetic modules that were present at least 149 million years ago in the most recent common ancestor of living angiosperms. We began by using laser-assisted microdissection to generate novel transcriptomic data from female flower tissues of Amborella trichopoda, the likely sister to all other living angiosperms. We then employed a gene-expression clustering method, followed by a custom procedure to compare genetic modules on the basis of gene orthology between Amborella and the molecular-genetic model angiosperm Arabidopsis thaliana. Using this protocol, we succeeded in identifying nine major genetic modules that appear to have conserved their expression dynamics from an early stage in angiosperm evolution. The genes of these modules, representing over 5000 orthogroups, include around one third of those known to control female reproductive development in Arabidopsis. Our study constitutes a proof of concept for the comparison of transcriptomic data between widely diverged plant species and represents a first step in the large-scale analysis of gene expression dynamics in a macro-evolutionary context.
Is Prehospital Ketamine Associated With a Change in the Prognosis of PTSD?
Authors: Melcer Ted , Walker G Jay , Dye Judy L , Walrath Benjamin , MacGregor Andrew J , Perez Katheryne , Galarneau Michael R 
Published: February 2022

ABSTRACT

Introduction
Ketamine is an alternative to opioids for prehospital analgesia following serious combat injury. Limited research has examined prehospital ketamine use, associated injuries including traumatic brain injury (TBI) and PTSD outcomes following serious combat injury.

Materials and Methods
We randomly selected 398 U.S. service members from the Expeditionary Medical Encounter Database who sustained serious combat injuries in Iraq and Afghanistan, 2010-2013. Of these 398 patients, 213 individuals had charted prehospital medications. Clinicians reviewed casualty records to identify injuries and all medications administered. Outcomes were PTSD diagnoses during the first year and during the first 2 years postinjury extracted from military health databases. We compared PTSD outcomes for patients treated with either (a) prehospital ketamine (with or without opioids) or (b) prehospital opioids (without ketamine).

Results
Fewer patients received prehospital ketamine (26%, 56 of 213) than only prehospital opioids (69%, 146 of 213) (5%, 11 of 213 received neither ketamine nor opioids). The ketamine group averaged significantly more moderate-to-serious injuries, particularly lower limb amputations and open wounds, compared with the opioid group (Ps &lt; .05). Multivariable regressions showed a significant interaction between prehospital ketamine (versus opioids) and TBI on first-year PTSD (P = .027). In subsequent comparisons, the prehospital ketamine group had significantly lower odds of first-year PTSD (OR = 0.08, 95% CI [0.01, 0.71], P = .023) versus prehospital opioids only among patients who did not sustain TBI. We also report results from separate analyses of PTSD outcomes among patients treated with different prehospital opioids only (without ketamine), either morphine or fentanyl.

Conclusions
The present results showed that patients treated with prehospital ketamine had significantly lower odds of PTSD during the first year postinjury only among patients who did not sustain TBI. These findings can inform combat casualty care guidelines for use of prehospital ketamine and opioid analgesics following serious combat injury.
Identifying blue large amplitude pulsators from Gaia DR2 and ZTF DR3
Authors: McWhirter Paul Ross , Lam Marco C 
Published: February 2022

ABSTRACT
Blue large amplitude pulsators (BLAPs) are hot, subluminous stars undergoing rapid variability with periods of under 60 min. They have been linked with the early stages of pre-white dwarfs and hot subdwarfs. They are a rare class of variable star due to their evolutionary history within interacting binary systems and the short time-scales relative to their lifetime in which they are pulsationally unstable. All currently known BLAPs are relatively faint (15–19 mag) and are located in the Galactic plane. These stars have intrinsically blue colours but the large interstellar extinction in the Galactic plane prevents them from swift identification using colour-based selection criteria. In this paper, we correct the Gaia G-band apparent magnitude and GBP − GRP colours of 89.6 million sources brighter than 19 mag in the Galactic plane with good quality photometry combined with supplementary all-sky data totalling 162.3 million sources. Selecting sources with colours consistent with the known population of BLAPs and performing a cross-match with the Zwicky Transient Facility (ZTF) DR3, we identify 98 short period candidate variables. Manual inspection of the period-folded light curves reveals 22 candidate BLAPs. Of these targets, 6 are consistent with the observed periods and light curves of the known BLAPs, 10 are within the theoretical period range of BLAPs, and 6 are candidate high-gravity BLAPs. We present follow-up spectra of 21 of these candidate sources and propose to classify one of them as a BLAP, and tentatively assign an additional eight of them as BLAPs for future population studies.