bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conference report: Second annual young researcher forum of the Japan Society of Medical and Pharmaceutical Sciences for Traditional Medicine
Authors: Yoshino Tetsuhiro , Yoneda Taichi , Yang Ximeng , Nishihara Masakazu , Ogawa Keiko , Kitamura Masashi , Ando Hirokazu , Nakashima Souichi , Horie Ichiro 
Published: August 2022

AbstractFive young researchers presented their studies in the field of traditional Japanese herbal medicine (Wakan‐yaku) at the second annual young researcher forum entitled “Growing Research Buds by Young Investigators,” held during the annual meeting of the Japan Society of Medical and Pharmaceutical Sciences for Traditional Medicine 2021. Mr. Yoneda presented exploratory research on new pharmaceutical seed compounds from garlic (Allium sativum). Dr. Yang investigated whether diosgenin promoted proper axonal regeneration in the brain using a mouse model of Alzheimer's disease. Her study clarified the molecular mechanisms of the diosgenin‐driven accurate pathfinding of injured axons. Mr. Nishihara presented his research on promoting the effective utilization of Phellodendron trees in Nara Prefecture. His research team evaluated the possibility of short‐term cultivation, screened for useful components in the leaves. Dr. Ogawa used the Japanese Adverse Drug Event Report (JADER) database, which contains reports of side effects associated with drug treatment, to demonstrate the effectiveness of hokizai in reducing side effects, particularly those of anticancer drugs. Dr. Kitamura reported that Cnidium monnieri (L.) Cusson fruit extract and the isolated active ingredients, osthol and imperatorin, strongly inhibited the activity of furin‐like enzymes. Further screening indicated that root extracts of Paeonia lactiflora Pall. and Scutellaria baicalensis Georgi suppressed cell syncytial formation by the S protein. The event was held online, and we had 86 participants. We can improve this forum in terms of format, frequency, and publicity activities; however, we hope to hold this forum in 2022 and in the future.
Noncoding RNAs in oral cancer
Authors: Balakittnen Jaikrishna , Weeramange Chameera Ekanayake , Wallace Daniel F. , Duijf Pascal H. G. , Cristino Alexandre S. , Kenny Liz , Vasani Sarju , Punyadeera Chamindie 
Published: August 2022

AbstractOral cancer (OC) is the most prevalent subtype of cancer arising in the head and neck region. OC risk is mainly attributed to behavioral risk factors such as exposure to tobacco and excessive alcohol consumption, and a lesser extent to viral infections such as human papillomaviruses and Epstein–Barr viruses. In addition to these acquired risk factors, heritable genetic factors have shown to be associated with OC risk. Despite the high incidence, biomarkers for OC diagnosis are lacking and consequently, patients are often diagnosed in advanced stages. This delay in diagnosis is reflected by poor overall outcomes of OC patients, where 5‐year overall survival is around 50%. Among the biomarkers proposed for cancer detection, noncoding RNA (ncRNA) can be considered as one of the most promising categories of biomarkers due to their role in virtually all cellular processes. Similar to other cancer types, changes in expressions of ncRNAs have been reported in OC and a number of ncRNAs have diagnostic, prognostic, and therapeutic potential. Moreover, some ncRNAs are capable of regulating gene expression by various mechanisms. Therefore, elucidating the current literature on the four main types of ncRNAs namely, microRNA, lncRNA, snoRNA, piwi‐RNA, and circular RNA in the context of OC pathogenesis is timely and would enable further improvements and innovations in diagnosis, prognosis, and treatment of OC.This article is categorized under:
RNA in Disease and Development > RNA in Disease
RNA in Disease and Development > RNA in Development
The Relationship Between Teaching and Research in the Italian University System
Authors: Carillo Maria Rosaria , Sapio Alessandro , Venittelli Tiziana 
Published: August 2022

AbstractWe study the relationship between the quality of research and teaching in the Italian university system, at the study program level. We run a cross-sectional econometric analysis by using a very rich dataset collected by the Italian National Agency for the Evaluation of Universities and Research Institutes on the BA and MA-level degrees of all universities in Italy in the academic year 2016/2017. We find that a positive relationship between teaching quality and research performance emerges if we take account of yardstick competition among study programs belonging to the same department. Indeed, previous theoretical results suggest that, despite the individual trade-off between teaching and research faced by individual academics, in multi-unit universities adopting a budget sharing rule based on both research performance and number of students, the negative relation between teaching and research is reduced or even completely counterbalanced. We find a confirmation of this hypothesis by proxying yardstick competition with the number of study programs activated per department. However, the teaching–research relationship is positive and stronger where study programs are relatively few and immediately comparable by the department managers. Such results emerge more strongly in MA-level degrees, where teaching is more aligned with individual research interests.
Precision neuro-oncology: a pilot analysis of personalized treatment in recurrent glioma
Authors: Lazaridis Lazaros , Schmidt Teresa , Oster Christoph , Blau Tobias , Pierscianek Daniela , Siveke Jens T. , Bauer Sebastian , Schildhaus Hans-Ulrich , Sure Ulrich , Keyvani Kathy , Kleinschnitz Christoph , Stuschke Martin , Herrmann Ken , Deuschl Cornelius , Scheffler Björn , Kebir Sied , Glas Martin 
Published: August 2022

Abstract
Purpose
When brain cancer relapses, treatment options are scarce. The use of molecularly matched targeted therapies may provide a feasible and efficacious way to treat individual patients based on the molecular tumor profile. Since little information is available on this strategy in neuro-oncology, we retrospectively analyzed the clinical course of 41 patients who underwent advanced molecular testing at disease relapse.

Methods
We performed Sanger sequencing, targeted next generation sequencing, and immunohistochemistry for analysis of potential targets, including programmed death ligand 1, cyclin D1, phosphorylated mechanistic target of rapamycin, telomerase reverse transcriptase promoter mutation, cyclin-dependent kinase inhibitor 2A/B deletion, or BRAF-V600E mutation. In selected patients, whole exome sequencing was conducted.

Results
The investigation included 41 patients, of whom 32 had isocitrate dehydrogenase (IDH) wildtype glioblastoma. Molecular analysis revealed actionable targets in 31 of 41 tested patients and 18 patients were treated accordingly (matched therapy group). Twenty-three patients received molecularly unmatched empiric treatment (unmatched therapy group). In both groups, 16 patients were diagnosed with recurrent IDH wildtype glioblastoma. The number of severe adverse events was comparable between the therapy groups. Regarding the IDH wildtype glioblastoma patients, median progression-free survival (mPFS) and median overall survival (mOS) were longer in the matched therapy group (mPFS: 3.8 versus 2.0 months, p = 0.0057; mOS: 13.0 versus 4.3 months, p = 0.0357).

Conclusion
These encouraging data provide a rationale for molecularly matched targeted therapy in glioma patients. For further validation, future study designs need to additionally consider the prevalence and persistence of actionable molecular alterations in patient tissue.
Optical coherence tomography and convolutional neural networks can differentiate colorectal liver metastases from liver parenchyma ex vivo
Authors: Amygdalos Iakovos , Hachgenei Enno , Burkl Luisa , Vargas David , Goßmann Paul , Wolff Laura I. , Druzenko Mariia , Frye Maik , König Niels , Schmitt Robert H. , Chrysos Alexandros , Jöchle Katharina , Ulmer Tom F. , Lambertz Andreas , Knüchel-Clarke Ruth , Neumann Ulf P. , Lang Sven A. 
Published: August 2022

Abstract
Purpose
Optical coherence tomography (OCT) is an imaging technology based on low-coherence interferometry, which provides non-invasive, high-resolution cross-sectional images of biological tissues. A potential clinical application is the intraoperative examination of resection margins, as a real-time adjunct to histological examination. In this ex vivo study, we investigated the ability of OCT to differentiate colorectal liver metastases (CRLM) from healthy liver parenchyma, when combined with convolutional neural networks (CNN).

Methods
Between June and August 2020, consecutive adult patients undergoing elective liver resections for CRLM were included in this study. Fresh resection specimens were scanned ex vivo, before fixation in formalin, using a table-top OCT device at 1310 nm wavelength. Scanned areas were marked and histologically examined. A pre-trained CNN (Xception) was used to match OCT scans to their corresponding histological diagnoses. To validate the results, a stratified k-fold cross-validation (CV) was carried out.

Results
A total of 26 scans (containing approx. 26,500 images in total) were obtained from 15 patients. Of these, 13 were of normal liver parenchyma and 13 of CRLM. The CNN distinguished CRLM from healthy liver parenchyma with an F1-score of 0.93 (0.03), and a sensitivity and specificity of 0.94 (0.04) and 0.93 (0.04), respectively.

Conclusion
Optical coherence tomography combined with CNN can distinguish between healthy liver and CRLM with great accuracy ex vivo. Further studies are needed to improve upon these results and develop in vivo diagnostic technologies, such as intraoperative scanning of resection margins.
A systematic review of the mental health changes of children and young people before and during the COVID-19 pandemic
Authors: Kauhanen Laura , Wan Mohd Yunus Wan Mohd Azam , Lempinen Lotta , Peltonen Kirsi , Gyllenberg David , Mishina Kaisa , Gilbert Sonja , Bastola Kalpana , Brown June S. L. , Sourander Andre 
Published: August 2022

AbstractThere is increasing knowledge that the COVID-19 pandemic has had an impact on mental health of children and young people. However, the global evidence of mental health changes before compared to during the COVID-19 pandemic focusing on children and young people has not been systematically reviewed. This systematic review examined longitudinal and repeated cross-sectional studies comparing before and during COVID-19 pandemic data to determine whether the mental health of children and young people had changed before and during the COVID-19 pandemic. The Web of Science, PubMed, Embase and PsycINFO databases were searched to identify peer-reviewed studies that had been published in English and focused on children and young people between 0 and 24 years of age. This identified 21 studies from 11 countries, covering more than 96,000 subjects from 3 to 24 years of age. Pre-pandemic and pandemic data were compared. Most studies reported longitudinal deterioration in the mental health of adolescents and young people, with increased depression, anxiety and psychological distress after the pandemic started. Other findings included deteriorated negative affect, mental well-being and increased loneliness. Comparing data for pandemic and pre-pandemic periods showed that the COVID-19 pandemic may negatively impact the mental health of children and young people. There is an urgent need for high-quality research to address the impact, risks and protective factors of the pandemic on their mental health, as this will provide a good foundation for dealing with future health emergencies and other crises.
Red blood cell omega-3 fatty acids and attention scores in healthy adolescents
Authors: Pinar-Martí Ariadna , Fernández-Barrés Silvia , Gignac Florence , Persavento Cecilia , Delgado Anna , Romaguera Dora , Lázaro Iolanda , Ros Emilio , López-Vicente Mònica , Salas-Salvadó Jordi , Sala-Vila Aleix , Júlvez Jordi 
Published: August 2022

AbstractOmega-3 fatty acids are critical for brain function. Adolescence is increasingly believed to entail brain vulnerability to dietary intake. In contrast to the abundant research on the omega-3 docosahexaenoic acid (DHA) in cognition, research on DHA and attention in healthy adolescents is scarce. In addition, the role of alpha-linolenic acid (ALA), the vegetable omega-3 fatty acid, is unexplored. We examined associations between DHA and ALA and attention function among a healthy young population. In this cross-sectional study conducted in 372 adolescents (13.8 ± 0.9 years-old), we determined the red blood cell proportions of DHA and ALA by gas chromatography (objective biomarkers of their long-term dietary intake) and measured attention scores through the Attention Network Test. We constructed multivariable linear regression models to analyze associations, controlling for known confounders. Compared to participants at the lowest DHA tertile (reference), those at the highest DHA tertile showed significantly lower hit reaction time-standard error (higher attentiveness) (28.13 ms, 95% confidence interval [CI] =  – 52.30;  – 3.97), lower hit reaction time ( – 38.30 ms, 95% CI =  – 73.28;  – 3.33) and lower executive conflict response ( – 5.77 ms, 95% CI =  – 11.44;  – 0.09). In contrast, higher values were observed in those at the top tertile of ALA in hit reaction time compared to the lowest one (46.14 ms, 95% CI = 9.90; 82.34). However, a beneficial association was observed for ALA, with decreasing impulsivity index across tertiles. Overall, our results suggest that DHA (reflecting its dietary intake) is associated with attention performance in typically developing adolescents. The role of dietary ALA in attention is less clear, although higher blood levels of ALA appear to result in lower impulsivity. Future intervention studies are needed to determine the causality of these associations and to better shape dietary recommendations for brain health during the adolescence period.
Rangelands in a fragmented grass-dominated landscape are vulnerable to tree invasion from roadsides
Authors: Hogan Katharine F. E. , Fogarty Dillon T. , Ellerman Hugh , Fill Christopher T. , Morales D. , Seguin Baxter , Uden Daniel R. , Allen Craig R. 
Published: August 2022

AbstractRoadsides can be vectors for tree invasion within rangelands by bisecting landscapes and facilitating propagule spread to interior habitat. Current invasive tree management in North America’s Great Plains focuses on reducing on-site (i.e., interior habitat) vulnerability through on-site prevention and eradication, but invasive tree management of surrounding areas known to serve as invasion vectors, such as roadsides and public rights-of-ways, is sporadic. We surveyed roadsides for invasive tree propagule sources in a central Great Plains grassland landscape to determine how much of the surrounding landscape is potentially vulnerable to roadside invasion, and by which species, and thereby provide insights into the locations and forms of future landcover change. Invasive tree species were widespread in roadsides. Given modest seed dispersal distances of 100–200 m, our results show that roadsides have potential to serve as major sources of rangeland exposure to tree invasion, compromising up to 44% of rangelands in the study area. Under these dispersal distances, funds spent removing trees on rangeland properties may have little impact on the landscape’s overall vulnerability, due to exposure driven by roadside propagule sources. A key implication from this study is that roadsides, while often neglected from management, represent an important component of integrated management strategies for reducing rangeland vulnerability to tree invasion.
The differential cancer growth associated with anaesthetics in a cancer xenograft model of mice: mechanisms and implications of postoperative cancer recurrence
Authors: Iwasaki Masae , Zhao Hailin , Hu Cong , Saito Junichi , Wu Lingzhi , Sherwin Aislinn , Ishikawa Masashi , Sakamoto Atsuhiro , Buggy Donal , Ma Daqing 
Published: August 2022

Abstract
Anaesthetics may modify colorectal cancer cell biology which potentially affects long-term survival. This study aims to compare propofol and sevoflurane regarding with the direct anaesthetic effects on cancer malignancy and the indirect effects on host immunity in a cancer xenograft mode of mice. Cultured colon cancer cell (Caco-2) was injected subcutaneously to nude mice (day 1). Mice were exposed to either 1.5% sevoflurane for 1.5 h or propofol (20 μg g−1; ip injection) with or without 4 μg g−1 lipopolysaccharide (LPS; ip) from days 15 to 17, compared with those without anaesthetic exposure as controls. The clinical endpoints including tumour volumes over 70 mm3 were closely monitored up to day 28. Tumour samples from the other cohorts were collected on day 18 for PCR array, qRT-PCR, western blotting and immunofluorescent assessment. Propofol treatment reduced tumour size (mean ± SD; 23.0 ± 6.2mm3) when compared to sevoflurane (36.0 ± 0.3mm3) (p = 0.008) or control (23.6 ± 4.7mm3). Propofol decreased hypoxia inducible factor 1α (HIF1α), interleukin 1β (IL1β), and hepatocyte growth factor (HGF) gene expressions and increased tissue inhibitor of metalloproteinases 2 (TIMP-2) gene and protein expression in comparison to sevoflurane in the tumour tissue. LPS suppressed tumour growth in any conditions whilst increased TIMP-2 and anti-cancer neutrophil marker expressions and decreased macrophage marker expressions compared to those in the LPS-untreated groups. Our data indicated that sevoflurane increased cancer development when compared with propofol in vivo under non-surgical condition. Anaesthetics tested in this study did not alter the effects of LPS as an immune modulator in changing immunocyte phenotype and suppressing cancer development.
Dimethyl sulfoxide-free cryopreservation solution containing trehalose, dextran 40, and propylene glycol for therapy with human adipose tissue-derived mesenchymal stromal cells
Authors: Fujita Yasutaka , Nishimura Masuhiro , Wada Tamaki , Komori Natsuki , Otoi Takeshige 
Published: August 2022

AbstractWe evaluated a dimethyl sulfoxide (Me2SO)-free cryopreservation solution to freeze human adipose-derived mesenchymal stromal cells (hADSCs). In the first experiment, we compared the combined effects of 3% trehalose (3 T) and 5% dextran (5D) in lactated Ringer’s solution (LR) as a cryopreservation base solution containing 10% propylene glycol (PG). The cell viability of hADSCs immediately after thawing was significantly higher (p < 0.05) in LR supplemented with 3 T (LR-3 T) and with 3 T and 5D (LR-3 T-5D) than in LR. In the second experiment, we compared the cell characteristics of hADSCs freeze-thawed in LR-3 T-5D containing either 10% Me2SO or 10% PG. The cell viability, annexin V-positive ratio, colony-forming capacity, cell proliferation, cell surface antigen positivity, adipogenic differentiation, osteogenic differentiation, and genetic response to cytokine stimulation of hADSCs immediately after thawing were similar between the LR-3 T-5D containing 10% Me2SO and 10% PG. In the third experiment, we examined various concentrations of PG on the cell proliferative capacity of freeze-thawed hADSCs. The cell proliferative capacity of hADSCs frozen with LR-3 T-5D containing 2.5% to 5% PG was significantly higher (p < 0.05) than LR-3 T-5D containing 10% PG. Furthermore, the cell proliferative capacity of hADSCs frozen with LR-3 T-5D containing 4% PG was similar to that of fresh hADSCs. These results indicate that the combination of 3 T-5D in an LR solution as a basic solution is effective for post-thaw cell viability, and that the optimal concentration of PG to maintain the cell characteristics of hADSCs frozen with LR-3 T-5D is 2.5% to 5%, which is promising for cell therapy applications.
Robust subgroup discovery
Authors: Proença Hugo M. , Grünwald Peter , Bäck Thomas , van Leeuwen Matthijs 
Published: August 2022

AbstractWe introduce the problem ofrobust subgroup discovery, i.e., finding a set of interpretable descriptions of subsets that 1) stand out with respect to one or more target attributes, 2) are statistically robust, and 3) non-redundant. Many attempts have been made to mine eitherlocallyrobust subgroups or to tackle the pattern explosion, but we are the first to address both challenges at the same time from aglobalmodelling perspective. First, we formulate the broad model class of subgroup lists, i.e., ordered sets of subgroups, for univariate and multivariate targets that can consist of nominal or numeric variables, including traditional top-1 subgroup discovery in its definition. This novel model class allows us to formalise the problem of optimal robust subgroup discovery using the Minimum Description Length (MDL) principle, where we resort to optimal Normalised Maximum Likelihood and Bayesian encodings for nominal and numeric targets, respectively. Second, finding optimal subgroup lists is NP-hard. Therefore, we propose SSD++, a greedy heuristic that finds good subgroup lists and guarantees that the most significant subgroup found according to the MDL criterion is added in each iteration. In fact, the greedy gain is shown to be equivalent to a Bayesian one-sample proportion, multinomial, or t-test between the subgroup and dataset marginal target distributions plus a multiple hypothesis testing penalty. Furthermore, we empirically show on 54 datasets that SSD++ outperforms previous subgroup discovery methods in terms of quality, generalisation on unseen data, and subgroup list size.
People, Lemurs and Utilitarian Plants of the Littoral Forests in Southeast Madagascar
Authors: Račevska Elena , Hill Catherine M. , Longosoa Hoby Tsimijaly , Donati Giuseppe 
Published: August 2022

AbstractTropical forests support a diversity of plants. Many of them are threatened, emphasising that their shared use by people and wildlife may benefit their conservation. Litt forests of southeast Madagascar, home to seven threatened lemur species, provide the Antanosy people with natural resources. In the early 2000s, protected areas were established in two regions that historically incurred different levels of anthropogenic pressures: Sainte Luce and Mandena. We explored the local use of plants as medicine, construction materials and firewood, and examined the overlap of plants used by people and the red-collared brown lemur (Eulemur collaris), the largest lemur in this ecosystem and an important seed disperser. Between July and October 2018, 60 adults (30 women, 30 men) participated in semi-structured interviews. Our findings show 122 plants are locally used as medicines, 60 as construction materials, and 71 as firewood. Of all utilitarian plants, 52 were confirmed in this lemur’s diet. Sainte Luce participants reported they used a higher diversity of species in all three categories. Western medicines were available and preferred to medicinal plants, but the choice also often depended on the health condition being addressed. Firewood was preferred to charcoal for cooking. Resource restrictions of the protected areas has negatively affected the local people. Local ethnobotanical knowledge reflects the importance of plants, while differences in plant use of the two communities reflect differences in biodiversity and socio-economic circumstances. Emphasising this interdependence, especially in forest restoration, could be a path towards conservation of plants, lemurs, and people, as well as traditional livelihoods.