bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target
Authors: Abdelfattah Nourhan , Kumar Parveen , Wang Caiyi , Leu Jia-Shiun , Flynn William F. , Gao Ruli , Baskin David S. , Pichumani Kumar , Ijare Omkar B. , Wood Stephanie L. , Powell Suzanne Z. , Haviland David L. , Parker Kerrigan Brittany C. , Lang Frederick F. , Prabhu Sujit S. , Huntoon Kristin M. , Jiang Wen , Kim Betty Y. S. , George Joshy , Yun Kyuson 
Published: February 2022

AbstractA major rate-limiting step in developing more effective immunotherapies for GBM is our inadequate understanding of the cellular complexity and the molecular heterogeneity of immune infiltrates in gliomas. Here, we report an integrated analysis of 201,986 human glioma, immune, and other stromal cells at the single cell level. In doing so, we discover extensive spatial and molecular heterogeneity in immune infiltrates. We identify molecular signatures for nine distinct myeloid cell subtypes, of which five are independent prognostic indicators of glioma patient survival. Furthermore, we identify S100A4 as a regulator of immune suppressive T and myeloid cells in GBM and demonstrate that deleting S100a4 in non-cancer cells is sufficient to reprogram the immune landscape and significantly improve survival. This study provides insights into spatial, molecular, and functional heterogeneity of glioma and glioma-associated immune cells and demonstrates the utility of this dataset for discovering therapeutic targets for this poorly immunogenic cancer.
Histone H3K4me3 modification is a transgenerational epigenetic signal for lipid metabolism in Caenorhabditis elegans
Authors: Wan Qin-Li , Meng Xiao , Wang Chongyang , Dai Wenyu , Luo Zhenhuan , Yin Zhinan , Ju Zhenyu , Fu Xiaodie , Yang Jing , Ye Qunshan , Zhang Zhan-Hui , Zhou Qinghua 
Published: February 2022

AbstractAs a major risk factor to human health, obesity presents a massive burden to people and society. Interestingly, the obese status of parents can cause progeny’s lipid accumulation through epigenetic inheritance in multiple species. To date, many questions remain as to how lipid accumulation leads to signals that are transmitted across generations. In this study, we establish a nematode model of C. elegans raised on a high-fat diet (HFD) that leads to measurable lipid accumulation, which can transmit the lipid accumulation signal to their multigenerational progeny. Using this model, we find that transcription factors DAF-16/FOXO and SBP-1/SREBP, nuclear receptors NHR-49 and NHR-80, and delta-9 desaturases (fat-5, fat-6, and fat-7) are required for transgenerational lipid accumulation. Additionally, histone H3K4 trimethylation (H3K4me3) marks lipid metabolism genes and increases their transcription response to multigenerational obesogenic effects. In summary, this study establishes an interaction between a network of lipid metabolic genes and chromatin modifications, which work together to achieve transgenerational epigenetic inheritance of obesogenic effects.
An optogenetic approach for regulating human parathyroid hormone secretion
Authors: Liu Yunhui , Zhang Lu , Hu Nan , Shao Jie , Yang Dazhi , Ruan Changshun , Huang Shishu , Wang Liping , Lu William W. , Zhang Xinzhou , Yang Fan 
Published: February 2022

AbstractParathyroid hormone (PTH) plays crucial role in maintaining calcium and phosphorus homeostasis. In the progression of secondary hyperparathyroidism (SHPT), expression of calcium-sensing receptors (CaSR) in the parathyroid gland decreases, which leads to persistent hypersecretion of PTH. How to precisely manipulate PTH secretion in parathyroid tissue and underlying molecular mechanism is not clear. Here, we establish an optogenetic approach that bypasses CaSR to inhibit PTH secretion in human hyperplastic parathyroid cells. We found that optogenetic stimulation elevates intracellular calcium, inhibits both PTH synthesis and secretion in human parathyroid cells. Long-term pulsatile PTH secretion induced by light stimulation prevented hyperplastic parathyroid tissue-induced bone loss by influencing the bone remodeling in mice. The effects are mediated by light stimulation of opsin expressing parathyroid cells and other type of cells in parathyroid tissue. Our study provides a strategy to regulate release of PTH and associated bone loss of SHPT through an optogenetic approach.
Artificial neural network approach for multiphase segmentation of battery electrode nano-CT images
Authors: Su Zeliang , Decencière Etienne , Nguyen Tuan-Tu , El-Amiry Kaoutar , De Andrade Vincent , Franco Alejandro A. , Demortière Arnaud 
Published: February 2022

AbstractThe segmentation of tomographic images of the battery electrode is a crucial processing step, which will have an additional impact on the results of material characterization and electrochemical simulation. However, manually labeling X-ray CT images (XCT) is time-consuming, and these XCT images are generally difficult to segment with histographical methods. We propose a deep learning approach with an asymmetrical depth encode-decoder convolutional neural network (CNN) for real-world battery material datasets. This network achieves high accuracy while requiring small amounts of labeled data and predicts a volume of billions voxel within few minutes. While applying supervised machine learning for segmenting real-world data, the ground truth is often absent. The results of segmentation are usually qualitatively justified by visual judgement. We try to unravel this fuzzy definition of segmentation quality by identifying the uncertainty due to the human bias diluted in the training data. Further CNN trainings using synthetic data show quantitative impact of such uncertainty on the determination of material’s properties. Nano-XCT datasets of various battery materials have been successfully segmented by training this neural network from scratch. We will also show that applying the transfer learning, which consists of reusing a well-trained network, can improve the accuracy of a similar dataset.
Tea compound-saliva interactions and their correlations with sweet aftertaste
Authors: Chong Pik Han , Chen Jianshe , Yin Danting , Qin Lanxi 
Published: February 2022

AbstractHuigan is an important sensory attribute which is commonly used as a quality indicator evaluation of tea products. Previous studies showed a strong correlation between the lubrication behavior of saliva-tea compound mixture and the sensory perception of Huigan from trained panelists. This work was further designed to investigate how the effect of tea consumption on the rate of saliva secretion and its functional properties including total protein content of saliva (TPC), salivary α-amylase (AMY) and lipase activity (LP). A quartz crystal microbalance with dissipation monitoring (QCM-D) was applied to reveal the adsorption behavior of human whole saliva and how the salivary film is affected by the presence of tea compounds. Results showed a significant positive correlation among TPC, LP and Huigan intensity for subjects who are Huigan-sensitive. Compared to the desorption of salivary film, the desorption of saliva-EC/EGC (epicatechin/epigallocatechin) mixture from the gold surface by QCM-D observation showed a significant effect on Huigan intensity in sensitive group when comparing to the salivary layer (blank).
A three-terminal non-volatile ferroelectric switch with an insulator–metal transition channel
Authors: Vaidya Jaykumar , Kanthi R. S. Surya , Alam Shamiul , Amin Nazmul , Aziz Ahmedullah , Shukla Nikhil 
Published: February 2022

AbstractFerroelectrics offer a promising material platform to realize energy-efficient non-volatile memory technology with the FeFET-based implementations being one of the most area-efficient ferroelectric memory architectures. However, the FeFET operation entails a fundamental trade-off between the read and the program operations. To overcome this trade-off, we propose in this work, a novel device concept, Mott-FeFET, that aims to replace the Silicon channel of the FeFET with VO2- a material that exhibits an electrically driven insulator–metal phase transition. The Mott-FeFET design, which demonstrates a (ferroelectric) polarization-dependent threshold voltage, enables the read current distinguishability (i.e., the ratio of current sensed when the Mott-FeFET is in state 1 and 0, respectively) to be independent of the program voltage. This enables the device to be programmed at low voltages without affecting the ability to sense/read the state of the device. Our work provides a pathway to realize low-voltage and energy-efficient non-volatile memory solutions.
Ultrasound does not activate but can inhibit in vivo mammalian nerves across a wide range of parameters
Authors: Guo Hongsun , Offutt Sarah J. , Hamilton II Mark , Kim Yohan , Gloeckner Cory D. , Zachs Daniel P. , Alford Jamu K. , Lim Hubert H. 
Published: February 2022

AbstractUltrasound (US) has been shown to stimulate brain circuits, however, the ability to excite peripheral nerves with US remains controversial. To the best of our knowledge, there is still no in vivo neural recording study that has applied US stimulation to a nerve isolated from surrounding tissue to confirm direct activation effects. Here, we show that US cannot excite an isolated mammalian sciatic nerve in an in vivo preparation, even at high pressures (relative to levels recommended in the FDA guidance for diagnostic ultrasound) and for a wide range of parameters, including different pulse patterns and center frequencies. US can, however, reliably inhibit nerve activity whereby greater suppression is correlated with increases in nerve temperature. By prohibiting the nerve temperature from increasing during US application, we did not observe suppressive effects. Overall, these findings demonstrate that US can reliably inhibit nerve activity through a thermal mechanism that has potential for various health disorders, though future studies are needed to evaluate the long-term safety of therapeutic ultrasound applications.
Mycobacterium vaccae immunization in rats ameliorates features of age-associated microglia activation in the amygdala and hippocampus
Authors: Sanchez Kevin , Darling Jeffrey S. , Kakkar Reha , Wu Sienna L. , Zentay Andrew , Lowry Christopher A. , Fonken Laura K. 
Published: February 2022

AbstractAging and reduced exposure to environmental microbes can both potentiate neuroinflammatory responses. Prior studies indicate that immunization with the immunoregulatory and anti-inflammatory bacterium, Mycobacterium vaccae (M. vaccae), in aged rats limits neuroimmune activation and cognitive impairments. However, the mechanisms by which M. vaccae immunization ameliorates age-associated neuroinflammatory “priming” and whether microglia are a primary target remain unclear. Here, we investigated whether M. vaccae immunization protects against microglia morphological changes in response to aging. Adult (3 mos) and aged (24 mos) Fisher 344 × Brown Norway rats were immunized with either M. vaccae or vehicle once every week for 3 weeks. Aging led to elevated Iba1 immunoreactivity, microglial density, and deramification of microglia processes in the hippocampus and amygdala but not other brain regions. Additionally, aged rats exhibited larger microglial somas in the dorsal hippocampus, suggestive of a more activated phenotype. Notably, M. vaccae treatment ameliorated indicators of microglia activation in both the amygdala and hippocampus. While changes in morphology appeared to be region-specific, gene markers indicative of microglia activation were upregulated by age and lowered in response to M. vaccae in all brain regions evaluated. Taken together, these data suggest that peripheral immunization with M. vaccae quells markers of age-associated microglia activation.
Pilot study to determine differences in breath odour between cigarette and e-cigarette consumers
Authors: Dalrymple Annette , Coburn Steven , Brandt Marianne , Hardie George , Murphy James 
Published: February 2022

AbstractCigarette smoke is known to influence breath odour, but the effect of e-cigarettes is unknown. In this pilot study, we aimed to determine differences in breath odour between cigarette smokers (CSs) and e-cigarette consumers (ECs) in 33 healthy subjects: 11 CSs, 11 ECs and 11 non-smokers (NSs). Breath was sampled at baseline and following product use (CSs and ECs) or a waiting period (NSs) by eight trained odour judges using a six-point smoke intensity scale and a nine-point hedonic scale. We observed a statistically significant difference between CSs and ECs. Smoke intensity values were significantly higher in CSs than ECs and NSs, which were comparable both at baseline and after product use. In addition, hedonic values for CSs were significantly lower than both NSs and ECs after product use. These acute results indicate that cigarette and e-cigarette use results in significantly different breath. ECs breath has a reduced smoke odour and more pleasant aroma than CSs, and is comparable to NSs. This suggests there may be cosmetic benefits for CSs who quit smoking or switch to exclusive use of ECs. Further studies are required to understand the long-term effects of e-cigarette use on breath odour.
Face masks reduce interpersonal distance in virtual reality
Authors: Kroczek Leon O. H. , Böhme Stephanie , Mühlberger Andreas 
Published: February 2022

AbstractDuring the COVID-19 pandemic several behavioral measures have been implemented to reduce viral transmission. While these measures reduce the risk of infections, they may also increase risk behavior. Here, we experimentally investigate the influence of face masks on physical distancing. Eighty-four participants with or without face masks passed virtual agents in a supermarket environment to reach a target while interpersonal distance was recorded. Agents differed in wearing face masks and age (young, elderly). In addition, situational constraints varied in whether keeping a distance of 1.5 m required an effortful detour or not. Wearing face masks (both self and other) reduced physical distancing. This reduction was most prominent when keeping the recommended distance was effortful, suggesting an influence of situational constraints. Similarly, increased distances to elderly were only observed when keeping a recommended distance was effortless. These findings highlight contextual constraints in compensation behavior and have important implications for safety policies.
Ruddlesden–Popper 2D perovskites of type (C6H9C2H4NH3)2(CH3NH3)n−1PbnI3n+1 (n = 1–4) for optoelectronic applications
Authors: Rahil Mohammad , Ansari Rashid Malik , Prakash Chandra , Islam S. S. , Dixit Ambesh , Ahmad Shahab 
Published: February 2022

AbstractRuddlesden–Popper (RP) phase metal halide organo perovskites are being extensively studied due to their quasi-two dimensional (2D) nature which makes them an excellent material for several optoelectronic device applications such as solar cells, photo-detectors, light emitting diodes (LEDs), lasers etc. While most of reports show use of linear carbon chain based organic moiety, such as n-Butylamine, as organic spacer in RP perovskite crystal structure, here we report a new series of quasi 2D perovskites with a ring type cyclic carbon group as organic spacer forming RP perovskite of type (CH)2(MA)n−1PbnI3n+1; CH = 2-(1-Cyclohexenyl)ethylamine; MA = Methylamine). This work highlights the synthesis, structural, thermal, optical and optoelectronic characterizations for the new RP perovskite series n = 1–4. The demonstrated RP perovskite of type for n = 1–4 have shown formation of highly crystalline thin films with alternate stacking of organic and inorganic layers, where the order of PbI6 octahedron layering are controlled by n-value, and shown uniform direct bandgap tunable from 2.51 eV (n = 1) to 1.92 eV (n = 4). The PL lifetime measurements supported the fact that lifetime of charge carriers increase with n-value of RP perovskites [154 ps (n = 1) to 336 ps (n = 4)]. Thermogravimetric analysis (TGA) showed highly stable nature of reported RP perovskites with linear increase in phase transition temperatures from 257 °C (n = 1) to 270 °C (n = 4). Scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDAX) are used to investigate the surface morphology and elemental compositions of thin films. In addition, the photodetectors fabricated for the series using (CH)2(MA)n−1PbnI3n+1 RP perovskite as active absorbing layer and without any charge transport layers, shown sharp photocurrent response from 17 nA/cm2 for n = 1 to 70 nA/cm2 for n = 4, under zero bias and low power illumination conditions (470 nm LED, 1.5 mW/cm2). Furthermore, for lowest bandgap RP perovskite n = 4, (CH)2MA3Pb4I13 the photodetector showed maximum photocurrent density of ~ 508 nA/cm2 at 3 V under similar illumination condition, thus giving fairly large responsivity (46.65 mA/W). Our investigations show that 2-(1-Cyclohexenyl)ethylamine based RP perovskites can be potential solution processed semiconducting materials for optoelectronic applications such as photo-detectors, solar cells, LEDs, photobatteries etc.
Screening of rice drought-tolerant lines by introducing a new composite selection index and competitive with multivariate methods
Authors: Sabouri Atefeh , Dadras Ahmad Reza , Azari Matin , Saberi Kouchesfahani Abbas , Taslimi Mehraneh , Jalalifar Reza 
Published: February 2022

AbstractSelection and breeding for drought tolerance in rice have always been one of the leading objectives for rice breeders in water-deficient farming areas. In the present study, we applied the potential of recombinant inbred lines (RILs) population, which were derived from cross Shahpasand (Iranian landrace) and IR28, for the development of drought-tolerant rice lines. One hundred fifty-two lines along with five check varieties were investigated from 2017 to 2019 under non-stress and drought stress conditions. The yield reduction caused by drought based on overall mean during 2017, 2018, and 2019 were estimated to be 89.40, 57.95, and 35.31%, respectively. Using different statistical methods, certain lines, including L33, L90, and L109, which are considered as the best lines in most environments, were found to be promising for being utilized to increase rice drought tolerance. The averages of grain yield of the above-mentioned lines were respectively 6.45, 5.80, and 5.70 t ha−1 under non-stress condition, and respectively 2.77, 2.66, and 2.59 t ha−1 under drought stress condition. The yield reduction of the selected lines were significantly lower than that of others indicating the significant transgressive segregation. The results revealed using the combination of the best identified tolerance and susceptibility indices and GT-biplot are effective methods for screening superior lines. However, their utilization is not easy and requires specialized packages. For the first time, we introduced a new composite index as a combination of significant indices (CSI). CSI is in the form of a linear function of indices which effectiveness is determined by their correlation coefficient with grain yield. According to our results, using CSI, the identified rice drought-tolerant lines were in high agreement with those obtained by other methods, demonstrating that CSI is a simple but reliable composite index.
Exendin-4 alleviates steatosis in an in vitro cell model by lowering FABP1 and FOXA1 expression via the Wnt/-catenin signaling pathway
Authors: Khalifa Olfa , AL-Akl Neyla S. , Errafii Khaoula , Arredouani Abdelilah 
Published: February 2022

AbstractNon-alcoholic fatty liver disease (NAFLD) is the leading chronic liver disease worldwide. Agonists of the glucagon-like peptide-1 receptor (GLP-1R), currently approved to treat type 2 diabetes, hold promise to improve steatosis and even steatohepatitis. However, due to their pleiotropic effects, the mechanisms underlying their protective effect on NAFLD remain elusive. We aimed to investigate these mechanisms using an in vitro model of steatosis treated with the GLP-1R agonist Exendin-4 (Ex-4). We established steatotic HepG2 cells by incubating the cells with 400 µM oleic acid (OA) overnight. Further treatment with 200 nM Ex-4 for 3 h significantly reduced the OA-induced lipid accumulation (p < 0.05). Concomitantly, Ex-4 substantially reduced the expression levels of Fatty Acid-Binding Protein 1 (FABP1) and its primary activator, Forkhead box protein A1 (FOXA1). Interestingly, the silencing of β-catenin with siRNA abolished the effect of Ex-4 on these genes, suggesting dependency on the Wnt/β-catenin pathway. Additionally, after β-catenin silencing, OA treatment significantly increased the expression of nuclear transcription factors SREBP-1 and TCF4, whereas Ex-4 significantly decreased this upregulation. Our findings suggest that direct activation of GLP-1R by Ex-4 reduces OA-induced steatosis in HepG2 cells by reducing fatty acid uptake and transport via FABP1 downregulation.
Impact of lifestyle and comorbidities on seropositive rheumatoid arthritis risk from Korean health insurance data
Authors: Ro JunSoo , Kim Se Hee , Kim Hae-Rim , Lee Sang-Heon , Min Hong Ki 
Published: February 2022

AbstractRheumatoid arthritis (RA) is a systemic inflammatory arthritis in which primary prevention is key. However, the impact of lifestyle and comorbidities on RA development is unknown. Data from the Korean National Health Insurance Service (NHIS)-national sample cohort from 2002 to 2016 were used. At baseline, demographic characteristics, socioeconomic status, type of residential area, lifestyle behaviours (including exercise), and comorbidities (including the Charlson Comorbidity Index, CCI) were included. Cox regression analysis and Kaplan–Meier curves were used to evaluate the impact of lifestyle and comorbidities on seropositive RA occurrence. A total of 517,053 participants were included in the analysis for seropositive RA occurrence. Mean follow up duration was 71.5 and 142.3 person-month for seropositive RA occurrence group and non-occurrence group, respectively. Seropositive RA was diagnosed in 1,948 participants (0.37%) during follow-up. Cox regression analysis revealed that being aged between 40 and 79, a higher CCI, and hyperlipidemia resulted in elevated hazard ratios (HRs) for seropositive RA, whereas male gender, city residence, moderate alcohol consumption, high regular exercise and a BMI between 23 and 34.9 kg/m2 resulted in lower HRs. Using Korean NHIS data, the present study demonstrates that high-intensity regular physical exercise and moderate alcohol consumption are negatively associated with seropositive RA occurrence, which are modifiable lifestyle habits that might aid the primary prevention of seropositive RA.
Linkage between precipitation isotopes and biosphere-atmosphere interaction observed in northeast India
Authors: Chakraborty Supriyo , Burman Pramit Kumar Deb , Sarma Dipankar , Sinha Nitesh , Datye Amey , Metya Abirlal , Murkute Charuta , Saha Subodh K. , Sujith Krishnakumar , Gogoi Nirmali , Bora Abhijit , Maji Sabyasachi , Parua Dipak K. , Bera S. 
Published: February 2022

AbstractThe intra-seasonal variation in precipitation isotopes shows a characteristic declining trend over northeast India. As of now, no mechanism offers a consistent explanation of this trend. We have performed the isotopic analysis of precipitation (rain) and estimated net ecosystem exchange and latent heat fluxes using an eddy-covariance system in northeast India. Additionally, we have used a diagnostic model to determine the recycled rainfall in this region. We find a strong link between the enhanced ecosystem productivity and isotopic enrichment in rainwater during the premonsoon season. Subsequently, on the advent of monsoon, the Bay of Bengal generated moisture enters this region and depletes the isotopic values. Additionally, the regional-scale convective activities produce periodic lows in the precipitation isotopes. Contrary to the general understanding, our study shows that the internal factors, such as the local land-atmosphere interactions, rather than the external influences, play a significant role in governing the precipitation isotopes in northeast India.