bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early vs late histological confirmation of coeliac disease in children with new-onset type 1 diabetes
Authors: Kamrath Clemens , Tittel Sascha R. , Dunstheimer Desiree , Fröhlich-Reiterer Elke , Freff Markus , Böttcher Claudia , Scheffler Nadine , Lenze Stefanie , Gericke Elke , Thiele Susanne , Holl Reinhard W. 
Published: April 2022

Abstract
Aim
Screening for coeliac disease in asymptomatic children with new-onset type 1 diabetes is controversial. The aim of this study was to analyse whether the confirmation of coeliac disease in children with new-onset type 1 diabetes and positive screening results can be postponed.

Methods
This was a multicentre population-based cohort study based on the German/Austrian/Swiss/Luxembourgian Prospective Diabetes Follow-up Registry (Diabetes Patienten Verlaufsdokumentation [DPV]). Participants aged ≤18 years diagnosed with type 1 diabetes between 1995 and June 2021 and with elevated IgA tissue transglutaminase antibodies (anti-tTGA) at diabetes onset on screening for coeliac disease were included. We compared outcomes of participants with a diabetes duration of more than 1 year between those in whom coeliac disease was confirmed histologically within the first 6 months and those in whom coeliac disease was confirmed between 6 and 36 months after diabetes diagnosis.

Results
Of 92,278 children and adolescents with a diagnosis of type 1 diabetes, 26,952 (29.2%) had documented anti-tTGA data at diabetes onset. Of these, 2340 (8.7%) had an elevated anti-tTGA level. Individuals who screened positive were younger (median age 9.0 vs 9.8 years, p<0.001) and more often female (53.1% vs 44.4%, p<0.001). A total of 533 participants (22.8% of those who screened positive) had a documented biopsy, of whom 444 had documented histological confirmation of coeliac disease. Of 411 participants with biopsy-proven coeliac disease within the first 36 months of diabetes and follow-up data, histological confirmation was performed in 264 (64.2%) within the first 6 months and in 147 (35.8%) between 6 and 36 months after diabetes onset. At follow-up (median diabetes duration 5.3 years and 5.1 years, respectively), estimated median HbA1c levels (62.8 mmol/mol vs 62.2 mmol/mol [7.9% vs 7.8%]), cardiovascular risk markers (lipids, rate of microalbuminuria, blood pressure), rates of acute diabetes complications (diabetic ketoacidosis, severe hypoglycaemia) and the proportions of participants reaching anti-tTGA levels within the normal range did not differ between groups. Participants with delayed histological confirmation of coeliac disease showed no negative effects on growth or weight gain during the observation period.

Conclusions
Our study suggests that the histological confirmation of coeliac disease in asymptomatic individuals with new-onset type 1 diabetes could be postponed.

Graphical abstract
Modeling gravimetric signatures of third-degree ocean tides and their detection in superconducting gravimeter records
Authors: Sulzbach Roman , Wziontek Hartmut , Hart-Davis Michael , Dobslaw Henryk , Scherneck Hans-Georg , Van Camp Michel , Omang Ove Christian Dahl , Antokoletz Ezequiel D. , Voigt Christian , Dettmering Denise , Thomas Maik 
Published: April 2022

AbstractWe employ the barotropic, data-unconstrained ocean tide model TiME to derive an atlas for degree-3 tidal constituents including monthly to terdiurnal tidal species. The model is optimized with respect to the tide gauge data set TICON-td that is extended to include the respective tidal constituents of diurnal and higher frequencies. The tide gauge validation shows a root-mean-square (RMS) deviation of 0.9–1.3 mm for the individual species. We further model the load tide-induced gravimetric signals by two means (1) a global load Love number approach and (2) evaluating Greens-integrals at 16 selected locations of superconducting gravimeters. The RMS deviation between the amplitudes derived using both methods is below $$0.5 \ $$

0.5

nGal ($$1 \ $$

1

nGal $$= 0.01 \frac{\text {nm}}{\text {s}^2}$$

=
0.01

nm

s
2

) when excluding near-coastal gravimeters. Utilizing ETERNA-x, a recently upgraded and reworked tidal analysis software, we additionally derive degree-3 gravimetric tidal constituents for these stations, based on a hypothesis-free wave grouping approach. We demonstrate that this analysis is feasible, yielding amplitude predictions of only a few 10 nGal, and that it agrees with the modeled constituents on a level of 63–80% of the mean signal amplitude. Larger deviations are only found for lowest amplitude signals, near-coastal stations, or shorter and noisier data sets.
The polyene antifungal candicidin is selectively packaged into membrane vesicles in Streptomyces S4
Authors: Blackburn Sarah A. , Shepherd Mark , Robinson Gary K. 
Published: April 2022

AbstractIn recent years, much attention has been focused on the biogenesis, engineering and utilisation of outer membrane vesicles (OMVs) in Gram-negative bacteria in a range of environments and niches. While the precise mechanism of biogenesis is unknown, it is focused on the modification of the Gram-negative cell wall to facilitate blebbing at sites of weakness in and around the characteristically thin peptidoglycan layer within the periplasm. Here, we investigate the biogenesis of membrane vesicles (MVs) in the Gram-positive organism Streptomyces albus S4 (Seipke et al. J Bacteriol 193:4270–4271, 2011 and Fazal et al. Antonie Van Leeuwenhoek 113:511–520, 2020). The S. albus S4 strain is an antifungal (candicidin and antimycin) producing organism that was isolated from attine ants (Barke et al. BMC Biol 8:109, 2010). The biogenesis and characterisation of S. albus S4 MVs is demonstrated using the wild-type (WT) and mutant strains ΔantC (no antimycin production) ΔfscC (no candicidin production) and ΔantC ΔfscC (produces neither antimycin nor candicidin). Here, we have shown that the S. albus S4 strain produces MVs and that these are comprised of both specific protein profiles and secondary metabolites, with a clear demonstration of the ability to selectively package one antifungal (candicidin) but not the other (antimycin).
Pseudomonas putida and its close relatives: mixing and mastering the perfect tune for plants
Authors: Costa-Gutierrez Stefanie Bernardette , Adler Conrado , Espinosa-Urgel Manuel , de Cristóbal Ricardo Ezequiel 
Published: April 2022

Abstract
Plant growth–promoting rhizobacteria (PGPR) are a group of microorganisms of utmost interest in agricultural biotechnology for their stimulatory and protective effects on plants. Among the various PGPR species, some Pseudomonas putida strains combine outstanding traits such as phytohormone synthesis, nutrient solubilization, adaptation to different stress conditions, and excellent root colonization ability. In this review, we summarize the state of the art and the most relevant findings related to P. putida and its close relatives as PGPR, and we have compiled a detailed list of P. putida sensu stricto, sensu lato, and close relative strains that have been studied for their plant growth–promoting characteristics. However, the mere in vitro analysis of these characteristics does not guarantee correct plant performance under in vivo or field conditions. Therefore, the importance of studying adhesion and survival in the rhizosphere, as well as responses to environmental factors, is emphasized. Although numerous strains of this species have shown good performance in field trials, their use in commercial products is still very limited. Thus, we also analyze the opportunities and challenges related to the formulation and application of bioproducts based on these bacteria.

Key points
•The mini-review updates the knowledge on Pseudomonas putida as a PGPR.
• Some rhizosphere strains are able to improve plant growth under stress conditions.
• The metabolic versatility of this species encourages the development of a bioproduct.
“Fight-flight-or-freeze” – how Yarrowia lipolytica responds to stress at molecular level?
Authors: Celińska Ewelina 
Published: April 2022

Abstract

Yarrowia lipolytica is a popular yeast species employed in multiple biotechnological production processes. High resistance to extreme environmental conditions or metabolic burden triggered by synthetically forced over-synthesis of a target metabolite has its practical consequences. The proud status of an “industrial workhorse” that Y. lipolytica has gained is directly related to such a quality of this species. With the increasing amount of knowledge coming from detailed functional studies and comprehensive omics analyses, it is now possible to start painting the landscape of the molecular background behind stress response and adaptation in Y. lipolytica. This review summarizes the current state-of-art of a global effort in revealing how Y. lipolytica responds to both environmental threats and the intrinsic burden caused by the overproduction of recombinant secretory proteins at the molecular level. Detailed lists of genes, proteins, molecules, and biological processes deregulated upon exposure to external stress factors or affected by over-synthesis of heterologous proteins are provided. Specificities and universalities of Y. lipolytica cellular response to different extrinsic and intrinsic threats are highlighted.

Key points
• Y. lipolytica as an industrial workhorse is subjected to multiple stress factors.
• Cellular responses together with involved genes, proteins, and molecules are reviewed.
• Native stress response mechanisms are studied and inspire engineering strategies.
No impact of time to treatment initiation for head and neck cancer in a tertiary university center in 2003, 2008 and 2013
Authors: Kouka Mussab , Engelhardt Max , Wittig Andrea , Schultze-Mosgau Stefan , Ernst Thomas , Guntinas-Lichius Orlando 
Published: April 2022

Abstract
Background
This retrospective study investigated factors influencing time to treatment initiation (TTI) and the influence of TTI on overall survival (OS) of primary head and neck cancer (HNC) patients in cohorts from 2003, 2008 and 2013.

Methods
Two hundred and ninenty seven patients (78.8% men; median age: 62 years) were included. Kaplan–Meier analyses and multivariate Cox regression were performed to investigate OS.

Results
Mean times to treatment initiation (TTI) of 2003, 2008 and 2013 were 17.11 ± 18.00, 30.26 ± 30.08 and 17.30 ± 37.04 days, respectively. TTI for patients with T3/T4 tumors was higher than for T1/T2 (p = 0.010). In univariable analysis on OS, TTI > 5 days showed lower OS (p = 0.047). In multivariate analysis, longer TTI had no influence on lower OS [hazard ratio (HR) 1.236; 95% CI 0.852–1.791; p = 0.264], but male gender [HR 2.342; 95% CI 1.229–4.466; p = 0.010], increased age [HR 1.026; 95% CI 1.008–1.045; p = 0.005], M1 [HR 5.823; 95% CI 2.252–15.058; p = 0.003], hypopharynx tumor [HR 2.508; 95% CI 1.571–4.003; p <  0.001] and oral cavity tumor [HR 1.712; CI 1.101–2.661; p = 0.017]. The year of treatment showed no significant effect on OS.

Conclusion
Median TTI seemed to be very short compared to other studies. There was no clear trend in the impact of TTI on OS from 2003 to 2013.
Post-COVID-19 fatigue: the contribution of cognitive and neuropsychiatric symptoms
Authors: Calabria Marco , García-Sánchez Carmen , Grunden Nicholas , Pons Catalina , Arroyo Juan Antonio , Gómez-Anson Beatriz , Estévez García Marina del Carmen , Belvís Roberto , Morollón Noemí , Vera Igual Javier , Mur Isabel , Pomar Virginia , Domingo Pere 
Published: April 2022

AbstractFatigue in its many forms of physical, mental, and psychosocial exhaustion is a common symptom of post-COVID-19 condition, also known as “Long COVID.” Persistent fatigue in COVID-19 patients is frequently accompanied by cognitive dysfunction and neuropsychiatric symptoms; however, less is known about the relationships between these components of post-COVID-19 condition and fatigue itself. Consequently, the present study sought to (1) distinguish the types of fatigue experienced by participants, and (2) investigate whether cognitive deficits across various domains and neuropsychiatric conditions predicted these different types of fatigue. The study included 136 COVID-19 patients referred for neuropsychological evaluation due to cognitive complaints 8 months on average after SARS-CoV-2 infection. Measures included self-reported fatigue (physical, cognitive, and psychosocial), neuropsychiatric questionnaires (assessing symptoms of depression, anxiety, apathy, and executive functioning), a comprehensive neuropsychological assessment, and self-reported quality of life and everyday functioning. Results showed that reports of clinical significant fatigue were pervasive in our sample (82.3% of participants), with physical fatigue rated highest on average relative to the subscale maximum. Elevated levels of apathy, anxiety, and executive dysfunction in neuropsychiatric measures along with executive and attentional difficulties on cognitive tests were found to be consistently important predictors among different types of fatigue. This implicates both cognitive and neuropsychiatric symptoms as predictors of fatigue in post-COVID-19 condition, and stresses the importance of a holistic approach in assessing and considering potential treatment for COVID-19 patients experiencing fatigue.
The clinical utility of polygenic risk scores in genomic medicine practices: a systematic review
Authors: Kumuthini Judit , Zick Brittany , Balasopoulou Angeliki , Chalikiopoulou Constantina , Dandara Collet , El-Kamah Ghada , Findley Laura , Katsila Theodora , Li Rongling , Maceda Ebner Bon , Monye Henrietta , Rada Gabriel , Thong Meow-Keong , Wanigasekera Thilina , Kennel Hannah , Marimuthu Veeramani ,   the G2MC Evidence investigators, Williams Marc S. , Al-Mulla Fahd , Abramowicz Marc 
Published: April 2022

AbstractGenomic medicine aims to improve health using the individual genomic data of people to inform care. While clinical utility of genomic medicine in many monogenic, Mendelian disorders is amply demonstrated, clinical utility is less evident in polygenic traits, e.g., coronary artery disease or breast cancer. Polygenic risk scores (PRS) are subsets of individual genotypes designed to capture heritability of common traits, and hence to allow the stratification of risk of the trait in a population. We systematically reviewed the PubMed database for unequivocal evidence of clinical utility of polygenic risk scores, using stringent inclusion and exclusion criteria. While we identified studies demonstrating clinical validity in conditions where medical intervention based on a PRS is likely to benefit patient outcome, we did not identify a single study demonstrating unequivocally such a benefit, i.e. clinical utility. We conclude that while the routine use of PRSs hold great promise, translational research is still needed before they should enter mainstream clinical practice.
Non-incremental response evaluation in geometrically nonlinear structural dynamics using a space-time stiffness operator
Authors: Arjoune Tahar , Markert Bernd , Bamer Franz 
Published: April 2022

AbstractThis contribution presents a proper generalized decomposition-based nonlinear solver for an efficient solution of geometrically nonlinear dynamic problems. The solution is built as a sum of dyadic products of space and time modes, and this sum of so-called enrichments is truncated when the required accuracy is achieved. In the proposed algorithm, we apply a consistent linearization of the residual vectors around the currently known solution over the whole space-time domain. At first, the set of vectorized tangent stiffness matrices is separated in space and time using the singular value decomposition. Then, the left and right singular vectors are reshaped into matrices to separate the space-time stiffness operator. The latter can be incorporated into the alternating fixed-point algorithm to compute couples of space and time modes. Numerical examples of a two-dimensional geometrically exact beam model demonstrate the accuracy, efficiency, and limits of the method.
A dominant clonal lineage of Streptococcus uberis in cattle in Germany
Authors: Fenske Linda , Noll Irene , Blom Jochen , Ewers Christa , Semmler Torsten , Fawzy Ahmad , Eisenberg Tobias 
Published: April 2022

AbstractBovine mastitis causes enormous economic losses in the dairy industry withStreptococcus uberisas one of the most common bacterial pathogens causing clinical and subclinical variations. In most cases mastitis can be cured by intramammary administration of antimicrobial agents. However, the severity of the clinical manifestations can vary greatly from mild to severe symtoms. In this study, a comparative genomic analysis of 24S. uberisisolates from three dairy farms in Germany, affected by different courses of infection was conducted. While there were sporadic mild infections in farm A and B, a large number of infections were observed within a very short period of time in farm C. The comparison of virulence genes, antimicrobial resistance genes and prophage regions revealed no features that might be responsible for this severe course. However, almost all isolates from farm C showed the same, novel MLST profile (ST1373), thus a clonal outbreak cannot be excluded, whereby the actual reason for the particular virulence remains unknown. This study demonstrates the importance of extensive metagenomic studies, including the host genomes and the environment, to gain further evidence on the pathogenicity ofS. uberis.
Coaching While Waiting for Autism Spectrum Disorder Assessment: A Pilot Feasibility Study for a Randomized Controlled Trial on Occupational Performance Coaching and Service Navigation
Authors: Bernie Charmaine , Williams Katrina , Graham Fiona , May Tamara 
Published: April 2022

Abstract
Aim
To determine whether short-phase Occupational Performance Coaching combined with service navigation support is feasible for families waiting for autism assessment.

Method
A pilot feasibility study was conducted using a blinded randomization procedure that allocated participants to one of three trial arms: (1) face-to-face coaching, (2) videoconference coaching, and (3) usual care. Outcomes included a retention aim of 70–80%, goal attainment and secondary standardised measures of adaptive behaviour, social skills, parenting stress, service access and family quality of life.

Results
Caregivers and children (n = 16, child mean age of 3 years 7 months) were recruited following referral for an autism assessment. Retention was 75%, with change scores in performance and satisfaction of selected goals higher in the intervention groups than the usual care group.

Interpretation
Findings support progression to a future randomized controlled trial assessing intervention efficacy.
La (III) Separation by Tri Octyl Phosphine Oxide (Cyanex 921) Based on Amberlite Xad-4 Chelating Resin
Authors: Fatah A. I. L. Abd El , Elashry Soaad M. 
Published: April 2022

AbstractThe novel core–shell type polymeric supports with accessible phosphorus groups were synthesised in the search for new reactive materials designed for the synthesis of functional resins. Amberlite XAD-4 adsorbent was impregnated with tri octyl phosphine oxides (Cyanex 921), which were then polymerized in the polymer carrier structure. The syntheses were evaluated by capturing FT-IR spectra, SEM micrographs, and analysing the sorption process. Batch studies were conducted to study the influence of some factors like pH, contact time, the metal ions concentration, and temperature on sorption efficiency of La (III) ions. The results showed that the optimum conditions were at pH equal to 0.5 and an equilibrium contact time of 30 min. According to the results of the sorption data analysis, the pseudo-second-order and Langmuir models were better fitted than the other estimated models. The sorption capacity of La (III) ions into impregnated resin as adsorbent martial was 54.25 mg g−1. The results revealed that the used adsorbent has been used successfully as a promising material for the elimination and recovery of La ions from the aqueous solutions. The impregnated resin exhibits a high chemical stability, reusability and fast equilibration. Further, the above procedure has been successfully employed for the application of real sample.
Effects of temporal floral resource availability and non-crop habitats on broad bean pollination
Authors: Eckerter Philipp W. , Albrecht Matthias , Bertrand Colette , Gobet Erika , Herzog Felix , Pfister Sonja C. , Tinner Willy , Entling Martin H. 
Published: April 2022

AbstractContextFlowering plants can enhance wild insect populations and their pollination services to crops in agricultural landscapes, especially when they flower before the focal crop. However, characterizing the temporal availability of specific floral resources is a challenge.ObjectivesDeveloping an index for the availability of floral resources at the landscape scale according to the specific use by a pollinator. Investigating whether detailed and temporally-resolved floral resource maps predict pollination success of broad bean better than land cover maps.MethodsWe mapped plant species used as pollen source by bumblebees in 24 agricultural landscapes and developed an index of floral resource availability for different times of the flowering season. To measure pollination success, patches of broad bean (Vicia faba), a plant typically pollinated by bumblebees, were exposed in the center of selected landscapes.ResultsHigher floral resource availability before bean flowering led to enhanced seed set. Floral resource availability synchronous to broad bean flowering had no effect. Seed set was somewhat better explained by land cover maps than by floral resource availability, increasing with urban area and declining with the cover of arable land.ConclusionsThe timing of alternative floral resource availability is important for crop pollination. The higher explanation of pollination success by land cover maps than by floral resource availability indicates that additional factors such as habitat disturbance and nesting sites play a role in pollination. Enhancing non-crop woody plants in agricultural landscapes as pollen sources may ensure higher levels of crop pollination by wild pollinators such as bumblebees.
Domestic violence reporting during the COVID-19 pandemic: evidence from Latin America
Authors: Perez-Vincent Santiago M. , Carreras Enrique 
Published: April 2022

AbstractThis article examines changes in the frequency and characteristics of domestic violence reports following the onset of the COVID-19 pandemic and the imposition of mobility restrictions in six Latin American countries. We find significantly different patterns between reports of psychological and physical violence, non-cohabitant and cohabitant violence, and across alternative reporting channels (domestic violence hotlines, emergency lines, and police reports). Calls to domestic violence hotlines soared, suggesting that this channel was best suited to respond to victims’ needs during the pandemic. In turn, calls to emergency lines and police complaints declined (especially in the first weeks of the pandemic), consistent with an increase in the perceived (relative) cost of using these channels. The results reveal how the pandemic altered domestic violence victims’ demand for institutional help and highlight the relevance of domestic violence hotlines as an accessible and valuable service.