bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cervical cancer screening and predictors of screening by diabetes status
Authors: Miller Eric A , Pinsky Paul 
Published: May 2022

Abstract
PurposeWomen with diabetes have lower survival rates after a cervical cancer diagnosis compared to women without diabetes. Pap smears and human papilloma virus (HPV) testing are highly effective screening tests for cervical cancer, therefore, it is important to know the prevalence of guideline-concordant screening among women with diabetes and understand if their predictors of screening differ. The purpose of this analysis was to assess guideline-concordant cervical cancer screening and predictors by diabetes status.MethodsWe used the 2019 National Health Interview Survey data, limited to women aged 21-65 years without a previous diagnosis of cancer, a hysterectomy, or diagnosed with diabetes in the year prior to the survey. We considered the Pap and HPV tests together and concordance as being tested within the past 3 years as part of a routine exam. We calculated weighted, adjusted prevalence and prevalence ratios (PRs) of screening concordance comparing women with diabetes to those without.ResultsThe unadjusted prevalence of concordant screening was 66.5% for women with diabetes compared to 73.3% for women without diabetes (PR=0.91 95%CI 0.84–0.98). In the model adjusting for age, race/ethnicity, education, income, marital status, employment, insurance coverage, usual source of healthcare, seeing a doctor in past year, and delaying medical care, the association was slightly attenuated and no longer statistically significant (PR=0.94 95%CI 0.88–1.02).ConclusionsCervical cancer screening concordance was lower in women with diabetes compared to those without overall but most barriers to screening appear to be from risk factors common to both diabetes and cervical cancer.
PICK1 mediates reactive oxygen species release and apoptosis in endotoxin-induced acute lung injury through interacting with PKCα and Ca 2+ /Calmodulin-activated Kinase II (CaMKII)
Authors: Qian Meizi , Zhu Yunrun , Wang Leilei , Mo Yunchang , Zhang Anqi , Yu Qimin , Wu Pinglei , Wang Junlu 
Published: May 2022

Abstract
Background: The pathophysiological mechanism of sepsis-related acute lung injury, which leads to a major cause of mortality in critically ill patients remains ambiguous. Our previous study demonstrated that PICK1 played a pivotal role in regulating antioxidants (Glutathione) synthesis via affecting xCT, however, its effects on oxidants was still unknown. This study aimed to explore whether PICK1 mediates reactive oxygen species release and apoptosis in endotoxin-induced acute lung injury, thereby funding effective treatments for ALI.Methods: In this study, male C57BL/6 mice or PICK1 knock out mice and mouse macrophage cell line, RAW264.7 were treated with LPS to establish endotoxin-induced acute lung injury model in vivo and in vitro. FSC-231 were used to disturb the interaction between PICK1 and PKCα. KN-93 were used to inhibit the activity of CaMKII. The alterations of protein PICK1, PKCα, CaMKII, p-CaMKII, BAX, Bcl-2, Cleaved-Caspase 3 were observed. Meanwhile, PICK1-PKCα complexes, PICK1-p-CaMKII complexes were detected. Cell Viability, ROS release, and cell apoptosis were examed as well. Results: We found that in endotoxin-related ALI, the content of PICK1 and p-CaMKII were augmented, while, PKCα were activated and declined. LPS stimulated the formation of PICK1-PKCα complexes, PICK1-p-CaMKII complexes. Moreover, PICK1 knock out exacerbate ROS release and apoptosis. Either PDZ or BAR domain disruption could mimic the effects of PICK1 knock out. Conclusions: Our results suggest that PICK1 mediates ROS release and apoptosis via interaction with PKCα and CaMKII.
Leaf traits of Cerrado trees vary according to season of leaf production
Authors: Araújo Igor , Vieira Tiffani C. S. , Cruz Wesley J. A. , Gloor Manuel U , Galbraith David R. , Junior Ben Hur Marimon , Marimon Beatriz S. 
Published: May 2022

Abstract
A large number of studies on plant functional traits assume time-invariant species-level mean trait values. Studies examining how leaf functional traits vary according to the season in which leaves were produced are very rare. In this study, we selected four species of trees and evaluated how morphological traits, and mesophyll and stomatal dimensions of leaves produced in the dry season differed from those produced in the wet season in a highly seasonal woodland savanna (cerradão) in the Amazonia-Cerrado transition. We found substantial differences in leaf traits between cohorts of leaves produced in both seasons, with those produced during the wet season characterized by more acquisitive trait values (e.g., high stomatal density, large stomatal pore size and high specific leaf area) than leaves of the same species produced in the dry season which were characterized by water conservative leaf traits. The magnitude of the difference for selected traits was remarkable – e.g., stomatal density of leaves produced in the dry season was on average four times greater than those produced in the wet season. Our results cast strong doubts on the use of mean specific values for syntheses of leaf traits and demonstrate that species in the Amazonia-Cerrado transition exhibit very high intraseasonal variation. Whether this high level of plasticity observed seasonally also applies to response to future climate change remains an open question.
Radioprotective effect of nanoniosome loaded by Mentha Pulegium essential oil on human peripheral blood mononuclear cells exposed to ionizing radiation
Authors: Hamzian Nima , Nickfarjam Abolfazl , Shams Ali , Haghiralsadat Fateme , Najmi-Nezhad Moslem 
Published: May 2022

Abstract

Introduction
: Despite the applications and advantages of ionizing radiation; there are many radiation risks to biological systems that are necessary to be reduced as much as possible. The present study aimed to assess the radioprotective effect of nanoniosomes loaded by Mentha Pulegium essential oil (MPEO-N) as a natural antioxidant on human peripheral blood mononuclear cells (PBMCs).
Materials and methods
MPEO-N nanoparticles were prepared by the lipid thin-film hydration method, and its physicochemical characteristics were analyzed. PBMCs were then irradiated with X-ray using a 6 MV linear accelerator at radiation doses of 25 and 200 cGy in the presence of non-toxic concentrations of MPEO-N nanoparticles (IC10). After 48 and 72 h of incubation, the radioprotective effect was investigated by measuring survival, apoptosis, and necrosis of PBMCs, using MTT assay and flow cytometry analysis.
Results
The hydrodynamic diameter and zeta potential of nanoniosomes were 106.0 nm and − 15.2 mV, respectively. The mean survival percentage of PBMCs showed a significant increase only at a radiation dose of 200 cGy compared with the control group. The percentages of apoptosis and necrosis of cells in the presence of MPEO-N nanoparticles at both radiation doses and incubation periods (48 and 72 hours) demonstrated a significant reduction compared with the control.
Conclusion
MPEO-N nanoparticles as a natural antioxidant, exhibited a favorable radioprotective effect by a significant reduction in the percentage of apoptosis and necrosis of irradiated PBMCs.
Analyzing the Adverse Events of Palliative Duodenal Stents in the Distal Duodenum: A Retrospective Chart Analysis
Authors: Reddy Srikar , Dailey Zachary , Al-Nakshabandi Ahmad , Chalhoub Walid 
Published: May 2022

Abstract
Self-expanding metal stents (SEMS) have demonstrated benefits in the setting of gastric outlet obstruction (GOO) by improving oral intake tolerance and reducing gastrointestinal symptoms. Endoscopic advances have enabled more minimally invasive procedures with fewer adverse events and are more cost-effective compared to surgeries. Our survey aimed to explore clinical outcomes in patients with palliative stents placed in the D3 and D4 portions of the duodenum. At a single tertiary care center from 2008 to 2021, there were 17 patients that underwent SEMS placement in the D3 and D4 portions of the duodenum, illustrating how rare this procedure is. There was 1 observed case of intestinal bleeding (hematemesis) within 30 days of stent placement (incidence 5.9%) and 1 observed case of stent migration (incidence of 5.9%). The total adverse event rate for duodenal stents placed in the D3 and D4 portions of the duodenum was found to be 11.7%. Duodenal stents placed in the D3 and D4 portions of the duodenum had comparable adverse event rates compared to stents placed in the D1 and D2 portions of the duodenum.
The effect of Phytosphingosine associated with toothbrushing on color change, surface roughness and microhardness of dental enamel - an in vitro and in situ study
Authors: de Arruda Carolina Noronha Ferraz , Vivanco Rocio Geng , Amorim Ayodele Alves , Ferreira Adriana Cavalcanti , Torrieri Rafaella Tonani , Bikker Floris Jacob , de Souza Fernanda de Carvalho Panzeri Pires 
Published: May 2022

Abstract
Objective. This study evaluated, in vitro and in situ, the effect of phytosphingosine (PHS) associated with toothbrushing regarding color stability, surface roughness, and microhardness. Methods. Sixty-four specimens of bovine teeth (6x6x2 mm) were separated in 8 groups (n=8): S+TB: PHS (spray) + toothbrushing; TB+S: toothbrushing + PHS (spray); I+TB: PHS (immersion) + toothbrushing; TB+I: toothbrushing + PHS (immersion); TB: toothbrushing; S: PHS spray; I: immersion in PHS solution and Saliva: immersion in saliva. Toothbrushing simulation (Mavtec, Brazil) was performed (356 rpm, 3.8 centimeters course by the toothbrush - Soft Tek) for 1, 7, 15, and 30 days. PHS remained in contact with specimens for 15 minutes. The specimens were evaluated before and after toothbrushing for color alteration (Easy Shade, VITA), surface roughness (Model SJ-201P Mitutoyo), and Knoop microhardness (HMV-2, Shimadzu Corporation). For the in situ analyses, 8 participants were recruited and received an intraoral device with 6 fragments of bovine enamel (6x6x2 mm). The properties evaluated were the same as those of the in vitro study. Participants were randomized following best results of in vitro tested protocols, for 15 days: TB; TB+S; I+TB. Data obtained by in vitro (two-way ANOVA, Tukey, p <.05) and in situ (one-way ANOVA, Tukey, p <.05) studies were analyzed. Results. The in vitro study showed a greater color change was found after 30 days. The greatest differences in surface roughness occurred between the initial value and after 1 day. Regarding microhardness, the highest values occurred after 15 and 30 days, which were similar. The in situ study showed greater color changes for the TB and I + TB, and greater surface roughness changes for TB and a similar increase in microhardness for the PHS protocols, which were higher than TB. Conclusions. Phytosphingosine leads to an increase in performance regarding color stability, surface roughness, and microhardness when applied. In general, the application of PHS after brushing showed a positive impact on its performance.Clinical relevance. Phytosphingosine showed to be interesting to compound prevention formulations in the dentistry field.
Evaluation of wound healing effect of curcumin loaded OPL carbon nanospheres embedded Chitosan membranes
Authors: M Roopesh , S Jyothi M , Velmurugan Rajendran , Hegde Gurumurthy , Keri Rangappa S , Soontarapa Khantong 
Published: May 2022

Abstract
Biowaste derived carbon and the biomaterial scaffolds are being used for wound healing are the focus of interest. Carbon nanospheres derived from oil palm leaves without any catalysts via pyrolysis were loaded with a traditional drug curcumin. The wound healing scaffolds were fabricated on the polypropylene non-woven fabric support using chitosan as the biopolymer matrix. Prepared carbon nanospheres and the scaffolds were characterized using ATR-IR and FESEM techniques. The wettability of scaffolds was examined to ensure the feasible moisture absorption ability, in vitro drug release profile and in vitro antibacterial activity against two bacterial. The in vivo wound healing feature of scaffolds was studied by excision wound model for MRSA infected wound. Measured % wound contraction and the bacterial count on wounds at regular time intervals proved that, the scaffold dressed with chitosan and curcumin loaded carbon nanoshperes showed an efficient reconstruction of skin through histopathological investigations.
Performance of serum Quad test in screening for fetal Down syndrome in a large-scale unselected population in a developing country
Authors: Chaipongpun Nitchanut , Tongsong Theera , Wanapirak Chanane , Sirichotiyakul Supatra , Tongprasert Fuanglada , Srisupun Kasemsri , Luewan Suchaya , Traisrisilp Kuntharee , Jatavan Phudit , Sirilert Sirinart 
Published: May 2022

Abstract
Objective: To assess the effectiveness of Quad test in the detection of fetal Down syndrome (DS) in routine practice among a large-scale population and, also, to compare the effectiveness of Quad test based on the Western reference model (WM) with ethnic correction and that based on Thai reference model (TM)Methods: Quad test was performed on 42,769 singleton pregnancies at 14 to 21 weeks of gestation, as a national policy with free-of-charge. The fetal risk of DS derived from Quad test was automatically computed based on WM and used in evaluating the effectiveness. Also, the fetal risk was calculated based on the TM.Results: Of 39,740 screened women with complete follow-ups, 74 fetuses were affected by DS. With WM, the detection and false positive rates were 81.1% and 7.2%, respectively, whereas the detection and false positive rates with TM were 87.8%, and 6.8%, respectively. According to the ROC curves, the performance of Quad test based on TM was slightly but significantly better than that based on WM (AUC of 0.959 vs 0.940, p=0.001).Conclusion: Quad test is highly effective in-service settings and suitable for developing countries and the effectiveness is even higher when based on ethnicity-specific reference model.
Contribution of Stipa bungeana root exudates to soil organic carbon fractions in the Loess Plateau
Authors: Li Yao , Yang Xuan , Bai Xuejuan , Wang Cong , Huang Yimei , An Shao-shan 
Published: May 2022

Abstract
Purpose Root exudates play an important role in carbon sequestration in grassland, but the contribution of root exudates to different soil organic carbon (SOC) fractions is poorly understood.Methods In this study, Stipa bungeana was used as the research subject and labeled with 13CO2 in an indoor culture experiment.Results After labeling, the content of SOC and 13C-SOC in rhizosphere soil was larger than that in bulk soil. In rhizosphere, the content of 13C-MBC and 13C-HC increase for 95.77% and 46.44% at the first day after labeling. At the 65 days, the content of 13C-SOC increase for 40.76%, but the 13C-DOC and13C-MBC decrease for 71.79% and 55.56% in rhizosphere.Conclusion The influence of root exudates on SOC was mainly in the early stage, and the influence on SOC gradually decreased. Root exudates had a rapid effect on active organic carbon fractions (MBC and DOC), also affect the humus carbon (HC) in the short term. This study reveals the mechanism of root exudates on SOC pool during plant growth.
Risk Analysis and Corrosion Prevention during Industrial Power Failure
Authors: Li Jia Yang , Denkenberger David 
Published: May 2022

Abstract
Modern civilisation depends on many different sectors functioning well and concurrently to run smoothly. This includes the energy sector, the transport sector, and the information technology sector. As such, the consequences can be catastrophic if these sectors were to fail due to some form of power outage or disaster. Possible causes include a high-altitude electromagnetic pulse from a nuclear bomb or a solar storm, a pandemic worse than COVID-19 causing people to be too fearful to work in critical industries, a cyber-attack, or an extreme natural disaster. Some work has been done on what could happen if such sectors are disabled, but virtually none on how to cope or to prepare for the loss. First, estimates of how long such a power outage will last were calculated. Next, scenarios where there is a power loss in industry for at least 5 to 25 years was explored. The consequences of such a scenario with regards to corrosion were considered. Possible strategies for what can be done to prepare for such scenarios were also explored. It was found that considering the design, removing corrosive components, and using coatings and packaging were the cheapest ways to reduce corrosion risks.
Estimation of hereditary fructose intolerance prevalence in Chinese population
Authors: Tang Meiling , Chen Xiang , Ni Qi , Lu Yulan , Wu Bingbing , Wang Huijun , Yin Zhaoqing , Zhou Wenhao , Dong Xinran 
Published: May 2022

Abstract
Background: Hereditary fructose intolerance (HFI) caused by aldolase B (ALDOB) reduction or deficiency is a rare inherited autosomal recessive (AR) disease that results in fructose metabolism disorder. The disease prevalence in the Chinese population is unknown, which leads to the lack of basis for the formulation of HFI screening and diagnosis strategy. Materials & Methods: From searching local cohort (Chinese Children’s Rare Disease Genetic Testing Clinical Collaboration System, CCGT), public databases (ClinVar and HGMD) and reviewing HFI-related literature (PubMed and Web of Science), we manually curated ALDOB pathogenic or likely-pathogenic (P/LP) variants according to ACMG guidelines. Allele frequency (AF) information from local CCGT, HuaBiao, and gnomAD database for ALDOB P/LP variants were used to estimate and the HFI prevalence in Chinese and other populations by the Bayesian framework. We collected the genotype and clinical characteristics of HFI patients from the CCGT database and published literature to study genotype-phenotype relationships. Result: In total 81 variants from ALDOB were curated as P/LP. The estimated Chinses HFI prevalence is approximately 1/504,678, which is similar to gnomAD-AFR (1/412,335) and SAS (1/465,278) population and much lower than NFE (1/23,147), FlN (1/55,539), AMR (1/132,801) and ASJ (1/263,150) populations. By analyzing the genetic characteristics of ALDOB in Chinese population, two variants (A338V, A338G) had significantly higher AF in Chinese population by comparing to NFE populations from gnomAD (all P-value<0.05). Five variants (A150P, A175D, N335K, R60*, R304Q) had significantly lower AF (all P-value<0.1). The results of genotype-phenotype association showed that patient carrying homozygous variant sites (especially A150P) were more likely to present nausea, and patient carrying two missense variant sites were more likely to present aversion to sweets and fruit (all P-value<0.05). Our research reveals that some gastrointestinal symptoms seem to be associated with genotypes.Conclusion: Chinese population had extremely low prevalence of HFI, with no need to add in current newborn screening project if consider medical costs. Genetic test strategy was suggested for early diagnoses.
Efficacy of immunochemotherapy compared with platinum-based chemotherapy and immunotherapy after tyrosine kinase inhibitor failure in advanced non-small cell lung cancer patients with EGFR mutation
Authors: Hata Tae , Sakaguchi Chikara , Hirano Keita , Kobe Hiroshi , Ishida Masaki , Nakano Takayuki , Tachibana Yusuke , Murai Junji , Enomoto Masamitsu , Tanaka Tomoki , Tamiya Nobuyo , Shiotsu Shinsuke , Takeda Takayuki , Yamada Tadaaki , Yokoyama Toshihide , Tsuchiya Michiko , Nagasaka Yukio 
Published: May 2022

Abstract
BackgroundPatients with epidermal growth factor receptor (EGFR)-mutated, advanced non-small cell lung cancer have received immunochemotherapy as one of the treatment options after tyrosine kinase inhibitor (TKI) failure. We examined the efficacy of immunochemotherapy in actual clinical practice and investigated subgroups of EGFR-mutant patients suitable for immunochemotherapy.MethodsWe conducted a multicenter retrospective cohort study of EGFR-mutant patients treated with atezolizumab-bevacizumab-carboplatin-paclitaxel (ABCP) therapy versus platinum-based chemotherapy (Chemo) and immune checkpoint inhibitor (ICI) monotherapy after EGFR-TKI therapy. Progression-free survival (PFS), according to underlying factors, including programmed death-ligand 1 (PD-L1) status, was also analyzed between the ABCP and Chemo groups.ResultsOf the 65 patients, 20, 37, and 8 received ABCP therapy, chemotherapy, and ICI monotherapy, respectively. The median PFS and overall survival periods in the ABCP, Chemo and ICI groups were 5.6 and 20.9 months, 5.4 and 22.1 months, and 1.2 and 9.2 months, respectively (PFS, P = 0.63; OS, P = 0.11). The median PFS periods of PD-L1-positive patients in the ABCP and Chemo groups were 6.9 and 4.7 months, respectively (hazard ratio [HR], 0.95; 95% confidence interval [CI], 0.41–2.17, P = 0.89). The median PFS of PD-L1-negative patients in the Chemo group (8.7 months) was significantly longer than that in the ABCP group (4.6 months) (HR, 0.34; 95% CI, 0.12–0.97, P = 0.04).ConclusionsThe effect of ABCP therapy was similar to chemotherapy in EGFR-mutant patients in a real-world setting. Particularly, for PD-L1-negative patients, platinum-based chemotherapy may be recommended over ABCP therapy.
Efficacy and safety of methylene blue in patients with vasodilatory shock: a systematic review and meta-analysis
Authors: Zhao Cong-Cong , Zhai Yu-Jia , Hu Zhen-Jie , Huo Yan , Li Zhi-Qiang , Zhu Gui-Jun 
Published: May 2022

Abstract
Background
The role of methylene blue (MB) in patients with vasodilatory shock is unclear. The purpose of this systematic review and meta-analysis was to evaluate the efficacy and safety of MB in patients with vasodilatory shock.
Methods
We searched MEDLINE at PubMed, Embase, Web of Science, Cochrane, CNKI, CBM and Wanfang Medical databases for all observational and intervention studies comparing the effect of MB and control in vasodilatory shock patients. This study was performed in accordance with the PRISMA statement. There were no language restrictions for inclusion.
Results
A total of 16 studies with 864 patients were included. Pooled data showed that MB as an adjunct to vasopressors significantly reduced mortality (odds ratio (OR) 0.54, 95% confidence interval (CI) 0.34 to 0.85, P = 0.008; I2 = 7%). Subgroup analyses found a significant difference in mortality between groups, favoring continuous infusion MB in septic shock patients. Moreover, the vasopressor requirement was significantly reduced in the MB group (mean difference (MD) -0.77, 95%CI -1.26 to -0.28, P = 0.002; I2 = 80%). For hemodynamics, MB increased the mean arterial pressure, heart rate and peripheral vascular resistance. For oxygen metabolism, MB reduced the level of lactate but had no effect on oxygen supply and oxygen consumption. MB had no effect on the other outcomes. No serious side effects were found.
Conclusions
Adjunct administration of MB was associated with lower mortality in patients with vasodilatory shock. This may be due to the ability of MB to decrease vasopressor requirements and the level of lactate and its ability to increase mean arterial pressure, heart rate, and peripheral vascular resistance.