bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of ethanol exposurein uteroon infant mice gut microbiotas assessed by nanopore and illumina sequencing
Authors: Pedroso-Roussado Cristiano , Guppy Fergus , Brissett Nigel , Bowler Lucas , Inacio Joao 
Published: December 2022

AbstractThe gut microbiome plays a vital role in host homeostasis and understanding of its biology is essential for a better comprehension of the etiology of disorders such as Foetal Alcohol Spectrum Disorder. Foetal Alcohol Spectrum Disorder represents a cluster of abnormalities including growth deficiencies and neurological impairments, which are not easily diagnosed nor treated. Here the effect of ethanol exposurein uteroon the gut microbial profiles of 16 infant mice (nine exposedin uteroand seven non-exposed) was assessed by targeted nanopore sequencing and Illumina sequencing approaches. The nanopore sequencing was implemented using MinION system targeting PCR-amplified amplicons made from the full-length 16S rRNA gene. The Illumina sequencing was performed using Miseq system targeting the V3-V4 region of the 16S rRNA gene. Ethanol exposure did not affect the microbial profiles. Several low prevalent taxa, likeAkkermansia muciniphila, were detected but further studies must be performed to detail the effect of ethanol exposure to these taxa since no clear pattern was detected throughout this study.ImportanceDetailed knowledge about the interactions between gut microbes and the developing nervous system is still scarce. Foetal Alcohol Spectrum Disorder represents a clinically relevant set of conditions with cumbersome diagnostic and treatment. In this work the microbial profiles of infant mice gut exposed to ethanolin uterowere analysed through third-generation Illumina and optimized next-generation nanopore sequencing technologies. The fungal (albeit not detected) and bacterial microbial profiles here obtained through nanopore and Illumina sequencing represent a technological and biological advancement towards a better comprehension of the microbial landscape in Foetal Alcohol Spectrum Disorder at early post-natal periods.
Molecular basis and design principles of a system for switchable front-rear polarity and directional migration
Authors: Menezes Carreira Luís António , Szadkowski Dobromir , Lometto Stefano , Hochberg Georg K.A. , Søgaard-Andersen Lotte 
Published: December 2022

AbstractDuring cell migration, front-rear polarity is spatiotemporally regulated; however, the underlying design of regulatory interactions vary. In rod-shapedMyxococcus xanthuscells, a spatial toggle switch dynamically regulates front-rear polarity. The polarity module establishes front-rear polarity by guaranteeing front pole-localization of the small GTPase MglA. Conversely, the Frz chemosensory system, by acting on the polarity module, causes polarity inversions. MglA localization depends on the RomR/RomX GEF and MglB/RomY GAP complexes that localize asymmetrically to the poles by unknown mechanisms. Here, we show that RomR and the MglB and MglC roadblock domain proteins generate a positive feedback by forming a RomR/MglC/MglB complex, thereby establishing the rear pole with high GAP activity that is non-permissive to MglA. MglA at the front engages in negative feedback that inhibits the RomR/MglC/MglB positive feedback allosterically, thus ensuring low GAP activity at this pole. These findings unravel the design principles of a system for switchable front-rear polarity.
Conformational restriction shapes inhibition of a multidrug efflux adaptor protein
Authors: Lewis Benjamin Russell , Uddin Muhammad R. , Moniruzzaman Mohammad , Kuo Katie M. , Higgins Anna J. , Shah Laila M. N. , Sobott Frank , Parks Jerry M. , Hammerschmid Dietmar , Gumbart James C. , Zgurskaya Helen I. , Reading Eamonn 
Published: December 2022

AbstractMembrane efflux pumps play a major role in bacterial multidrug resistance. The tripartite multidrug efflux pump system fromEscherichia coli, AcrAB-TolC, is a target for inhibition to lessen resistance development and restore antibiotic efficacy, with homologs in other ESKAPE pathogens. Here, we rationalize a mechanism of inhibition against the periplasmic adaptor protein, AcrA, using a combination of hydrogen/deuterium exchange mass spectrometry, cellular efflux assays, and molecular dynamics simulations. We define the structural dynamics of AcrA and find that an inhibitor can inflict long-range stabilisation across all four of its domains, whereas an interacting efflux substrate has minimal effect. Our results support a model where an inhibitor forms a molecular wedge within a cleft between the lipoyl and αβ domains of AcrA, diminishing its conformational transmission of drug-evoked signals from AcrB to TolC. This work provides molecular insights into multidrug adaptor protein function which could be valuable for developing antimicrobial therapeutics.
Color and gloss constancy under diverse lighting environments
Authors: Morimoto Takuma , Akbarinia Arash , Storrs Katherine , Cheeseman Jacob R. , Smithson Hannah E. , Gegenfurtner Karl R. , Fleming Roland W. 
Published: December 2022

AbstractWhen we look at an object, we simultaneously see how glossy or matte it is, how light or dark, and what color. Yet, at each point on the object’s surface, both diffuse and specular reflections are mixed in different proportions, resulting in substantial spatial chromatic and luminance variations. To further complicate matters, this pattern changes radically when the object is viewed under different lighting conditions. The purpose of this study was to simultaneously measure our ability to judge color and gloss using an image set capturing diverse object and illuminant properties. Participants adjusted the hue, lightness, chroma, and specular reflectance of a reference object so that it appeared to be made of the same material as a test object. Critically, the two objects were presented under different lighting environments. We found that hue matches were highly accurate, except for under a chromatically atypical illuminant. Chroma and lightness constancy were generally poor, but these failures correlated well with simple image statistics. Gloss constancy was particularly poor, and these failures were only partially explained by reflection contrast. Importantly, across all measures, participants were highly consistent with one another in their deviations from constancy. Although color and gloss constancy hold well in simple conditions, the variety of lighting and shape in the real world presents significant challenges to our visual system’s ability to judge intrinsic material properties.
Wnt-associated adult stem cell marker Lgr6 is required for osteogenesis and fracture healing
Authors: Doherty Laura , Wan Matthew , Peterson Anna , Youngstrom Daniel W. , King Justin S. , Kalajzic Ivo , Hankenson Kurt D. , Sanjay Archana 
Published: December 2022

AbstractDespite the remarkable regenerative capacity of skeletal tissues, nonunion of bone and failure of fractures to heal properly presents a significant clinical concern. Stem and progenitor cells are present in bone and become activated following injury; thus, elucidating mechanisms that promote adult stem cell-mediated healing is important. Wnt-associated adult stem marker Lgr6 is implicated in the regeneration of tissues with well-defined stem cell niches in stem cell-reliant organs. Here, we demonstrate that Lgr6 is dynamically expressed in osteoprogenitors in response to fracture injury. Using anLgr6-null mouse model, we find thatLgr6expression is necessary for maintaining bone volume and efficient postnatal bone regeneration in adult mice. Skeletal progenitors isolated fromLgr6-nullmice have reduced colony-forming potential and reduced osteogenic differentiation capacity due to attenuated cWnt signaling.Lgr6-null mice consist of a lower proportion of self-renewing stem cells. In response to fracture injury,Lgr6-nullmice have deficient proliferation of periosteal progenitors and reduced ALP activity. Further, analysis of bone regeneration phase and remodeling phase of fracture healing in Lgr6-null mice showed impaired endochondral ossification and reduced mineralization. We propose that in contrast to not being required for successful skeletal development Lgr6-positive cells have a direct role in endochondral bone repair.
ATP diffusional gradients are sufficient to maintain bioenergetic homeostasis in synaptic boutons lacking mitochondria
Authors: Kuznetsov Ivan A. , Kuznetsov Andrey V. 
Published: December 2022

AbstractPrevious work on mitochondrial distribution in axons has shown that approximately half of the presynaptic release sites do not contain mitochondria, raising the question of how the boutons that do not contain mitochondria are supplied with ATP. Here, we develop and apply a mathematical model to study this question. Specifically, we investigate whether diffusive transport of ATP is sufficient to support the exocytic functionality in synaptic boutons which lack mitochondria. Our results demonstrate that the difference in ATP concentration between a bouton containing a mitochondrion and a neighboring bouton lacking a mitochondrion is only approximately 0.4%, which is still 3.75 times larger than the ATP concentration minimally required to support synaptic vesicle release. This work therefore suggests that passive diffusion of ATP is sufficient to maintain the functionality of boutons which do not contain mitochondria.
Broadly applicable and accurate protein design by integrating structure prediction networks and diffusion generative models
Authors: Watson Joseph L. , Juergens David , Bennett Nathaniel R. , Trippe Brian L. , Yim Jason , Eisenach Helen E. , Ahern Woody , Borst Andrew J. , Ragotte Robert J. , Milles Lukas F. , Wicky Basile I. M. , Hanikel Nikita , Pellock Samuel J. , Courbet Alexis , Sheffler William , Wang Jue , Venkatesh Preetham , Sappington Isaac , Torres Susana Vázquez , Lauko Anna , De Bortoli Valentin , Mathieu Emile , Barzilay Regina , Jaakkola Tommi S. , DiMaio Frank , Baek Minkyung , Baker David 
Published: December 2022

AbstractThere has been considerable recent progress in designing new proteins using deep learning methods1–9. Despite this progress, a general deep learning framework for protein design that enables solution of a wide range of design challenges, includingde novobinder design and design of higher order symmetric architectures, has yet to be described. Diffusion models10,11have had considerable success in image and language generative modeling but limited success when applied to protein modeling, likely due to the complexity of protein backbone geometry and sequence-structure relationships. Here we show that by fine tuning the RoseTTAFold structure prediction network on protein structure denoising tasks, we obtain a generative model of protein backbones that achieves outstanding performance on unconditional and topology-constrained protein monomer design, protein binder design, symmetric oligomer design, enzyme active site scaffolding, and symmetric motif scaffolding for therapeutic and metal-binding protein design. We demonstrate the power and generality of the method, called RoseTTAFold Diffusion (RFdiffusion), by experimentally characterizing the structures and functions of hundreds of new designs. In a manner analogous to networks which produce images from user-specified inputs, RFdiffusionenables the design of diverse, complex, functional proteins from simple molecular specifications.
Non-micelle-like Amyloid Aggregate Stabilizes Amyloid β (1-42) Growth Nuclei Formation
Authors: Kurisaki Ikuo , Tanaka Shigenori 
Published: December 2022

AbstractProtein aggregate formations are essential processes to regulate biochemical networks in the cell, while anomalously formed aggregates such as amyloid fibrils cause serious neuronal diseases. It has been discussed for a quarter century that protein crowding milieus, such as micelle-like aggregates, promote the formation of growth nuclei, fibril-growth competent aggregates which trigger rapid growth of pathogenic amyloid fibrils, but the mechanisms are still elusive, in particular at microscopic level. In this study, we examined the long-standing problem by employing atomistic molecular dynamics simulations for amyloid β(1-42) (Aβ42), the paradigmatic amyloid-forming peptide. First, we constructed an atomistic model of Aβ42growth nuclei in Aβ42aggregate milieu, the pentameric Aβ42protomer dimer surrounded by 40 Aβ42monomers. Next, we simulated Aβ42monomer dissociation from the Aβ42growth nuclei and examined the effect of Aβ42aggregate milieu on the process. Aβ42aggregates spatially restrict Aβ42monomer dissociation pathways, while such spatial restriction itself does not significantly suppress Aβ42monomer dissociation from the growth nuclei. Rather, Aβ42aggregate milieus thermodynamically stabilize an Aβ42monomer binding to the growth edge by making atomic contacts with the monomer and contributes to stable formation of growth nuclei.A part of the aggregate milieu anchors dissociating monomer to the remaining part of growth nuclei, suggesting cooperative suppression of Aβ42monomer dissociation from Aβ42growth nuclei. Since the Aβ42aggregate milieu does not take a micelle-like configuration, we here discuss a new mechanism for stable formation of Aβ42growth nuclei in the presence of aggregate milieu.
Electrochemical study on using aminated MIL–101(Cr) as corrosion inhibitor for mild steel in acidic environment
Authors: Hassan Hassan M. , Alsohaimi Ibrahim H. , El-Aassar Mohamed R. , El-Hashemy Mohammed A. , Alraddadid Thamer S. 
Published: December 2022

Purpose
The purpose of this paper is to confirm the importance of using metal–organic frameworks (MOFs) in the field of corrosion control due to their potential use as corrosion inhibitors.

Design/methodology/approach
NH2–MIL–101(Cr), an amine-functionalized chromium-based MOF [Cr(III)-MOF], was prepared by solvothermal technique. Thereafter, Cr-MOF was used as an anticorrosion additive for mild steel (MS) in 1 m HCl solution. This inhibition behavior was tested by electrochemical tests including electrochemical impedance spectroscopy and potentiodynamic polarization (PDP).

Findings
Increasing the added amount of Cr-MOF enhances its inhibition performance, which attained 96.40% at 30 ppm. The obtained data from PDP measurements describe Cr-MOF as a mixed-type inhibitor. Based on SEM/EDS and FTIR analysis, the adsorption of Cr-MOF on the surface of MS that prevents MS corrosion has been demonstrated. Furthermore, Langmuir model is the most adequate adsorption isotherm for the obtained experimental data.

Originality/value
This study revealed that NH2–MIL–101(Cr), an amine-functionalized chromium-based MOF (Cr(III)-MOF), is a potential corrosion inhibitor for MS in 1 m HCl solution.
Live projects: a mixed-methods exploration of existing scholarship
Authors: Smith Sebastian G. , Dupre Karine , Crough Julie 
Published: December 2022

PurposeThis paper aims to investigate trends and themes within the literature pertaining to live projects, and in so doing, highlight possible areas of future exploration and research.Design/methodology/approachThis paper utilises a Systematic Quantitative Literature Review (SQLR) method, wherein keywords and phrases are entered into selected citation databases generating a reproducible list of literature. This is then refined using a specified list of criteria and read for relevance. The resulting literature forms the basis of qualitative and quantitative analyses and review.FindingsThe reviewed scholarship demonstrates a surge in publications since the early 2000s, with 75% of publications originating from the USA, Canada, or the UK Furthermore, themes related to live project definitions, outputs and rationales were examined, demonstrating that common factors such as “community”, “construction” and “pedagogy” are not mutually exclusive but tend to overlap, making the topic hard to define. These results also demonstrate a proclivity for projects with a built output. Barriers to live projects were also assessed, and it was found that administrative hurdles, such as time and budget constraints, were the biggest concern to live project practitioners. Finally, critical voices were examined and showed that live projects need to reflect on the nature of their engagement with the community.Research limitations/implicationsThis method, while capturing a substantial portion of the published scholarship, does not capture all live project literature due to limitations such as language and a strong focus on peer-reviewed publications. Furthermore, this research only captures literature that has been published. It does not reflect the variety and extent of live project activity occurring globally. For reasons such as unfamiliarity and inconsistencies with the use of live project terminologies, doubtless many unpublished live projects are conducted–yet not represented in these findings. This study may help live project execution by providing valuable examples of existing trends.Originality/valueThis paper captures the metadata from 110 live project publications, allowing for wide-ranging analysis, categorisation and discussion on the topic.
Ferrofluid squeeze film lubrication: surface roughness effect
Authors: Atlassi Khalil , Nabhani Mohamed , El Khlifi Mohamed 
Published: December 2022

Purpose
This study aims to investigate the combined effects of roughness and ferrofluid lubricant on finite journal bearing load capacity and squeeze time.

Design/methodology/approach
The stochastic theory of Christensen is applied to study the surface roughness effect. The Shliomis model is used to take into account the effects of the rotational viscosity of ferromagnetic particles and their magnetic moment. A finite wire located in the center of the shaft produces the applied magnetic field. A developed computing code allows predicting the effect of the surface roughness on the performance of the considered journal bearing. The good agreement with the results of the literature validates the used approach.

Findings
This study shows that unlike longitudinal roughness, the presence of transverse roughness makes the use of ferrofluid more beneficial in terms of increasing the performance of finite journal bearings. This increase is more significant for large relative eccentricities, which present an ideal confinement.

Originality/value
This study shows the effect of two surface roughness patterns on the squeezing performance of a finite journal bearing lubricated by a ferrofluid.
Electronic trust-building for hotel websites: a social exchange theory perspective
Authors: Nasrolahi Vosta Leila , Jalilvand Mohammad Reza 
Published: December 2022

Purpose
This study aims to contribute to the context of electronic trust (e-trust) research with an emphasis on the determination of tourists’ e-trust in hotel websites. This research used social exchange theory to elaborate how perceived attributes of a hotel website influence purchase behaviour of tourists. This area is often neglected because most studies focus on website users’ adoption or acceptance of other service industries. It is expected that trust-generating mechanisms have different impacts on the e-trust level in the hospitality services.

Design/methodology/approach
A comprehensive literature review identified the major antecedents and outcomes of e-trust. Data was collected from an online survey and 586 usable questionnaires were achieved. Structural equation modelling was used to examine the hypothesized model.

Findings
The results of this study demonstrated that the more tourists perceive influences positively, the more they are possibly to trust in hotel website. Findings revealed that perceptions about the attributes of hotel website, including security, privacy, usefulness, ease of use and compatibility are the main antecedents of trust in hotel website, which, in turn, lead to actual usage of the website for booking online.

Originality/value
This study is based on a large sample of tourists and broadens the understanding of e-trust in hotel websites by considering factors rarely discussed in prior tourism and hospitality literature.
A meta-analytical study on the role of religiosity on purchase intention in the theory of planned behavior
Authors: Kaur Jaspreet , Bhardwaj Neha , Fernandes Reynal , Vidya Vidya , Farooqui Nafees Akhter 
Published: December 2022

Purpose
Religion plays a crucial role as a sociocultural factor to assess consumer behavior. Stemming from the above, this study aims to analyze the impact of religion and ethnic concern on the purchase intention (PI) of consumers based on the theory of planned behavior.

Design/methodology/approach
The research method adopted for this study includes a meta-analysis of the extant literature for the past 20 years focusing on the relationship between religiosity and PI. Data of 24 values from 23 studies were used to assess the impact of religiosity on the PI of consumers.

Findings
The findings of this study indicate that religiosity has a strong impact on the PI of consumers. Further, this study identifies that location, sample size and product category play a vital role as moderators toward the relationship between religiosity and PI. This study identifies critical and pertinent implications for brands as they reach out to religious and cultural groups across various geographies, in the context of identifying target markets and adapting marketing strategies.

Originality/value
This study acts in response to the consistent call for research to focus on religion-related variables and fills the gap calling for empirical research into religiosity and its impact on PIs. This study makes notable theoretical, managerial and methodological contributions to the field.
A study on the coloration effectiveness of Chromolaena odorata on the worsted wool fabric
Authors: Pham Chau Thi Ngoc , Phan Hung Ngoc , Hoang Thao Thanh , Dao Tien Thi Thuy , Bui Huong Mai 
Published: December 2022

Purpose
The health and environmental hazards associated with synthetic dyes have led to a revival of natural dyes that are non-toxic, environmentally benign and coupled with various functions. The study aims to investigate and develop the potentiality of a popular herb called Chromolaena odorata (C. odorata) as a sustainable and stable dyestuff in textiles.

Design/methodology/approach
Natural colorant extracted from C. odorata leaves is used to dye the worsted fabric, which is one of the premier end-use of wool in fashion, via the padding method associated with pre-, simultaneous and post-mordanting with chitosan, tannic acid and copper sulfate pentahydrate. The effects of extraction, dyeing and mordanting processes on fabric’s color strength K/S and color difference ΔECMC are investigated via International Commission on Illumination’s L*a*b* color space, Fourier transform infrared spectroscopy, scanning electron microscope, color fastness to washing, rubbing, perspiration and light.

Findings
The results obtained indicate extraction with ethanol 90% with a solid/liquid ratio of 1:5 within 1 h, and coloration with a liquor ratio of 1:5 (pH 5) within 2 h under padding pressure of 0.3 MPa are the most effective for coloring worsted fabric.

Practical implications
The C. odorata’s application as a highly effective dyestuff possessing good colorimetric effectiveness has expanded this herb's economic potential, contributing partly to economic growth and adding value to wool in global supply chain.

Originality/value
C. odorata dyestuff has prevailed over other natural colorants because of its impressive color fastness against washing, rubbing, perspiration and especially color stability for pH change.