bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of reactive oxygen species and antioxidative enzymes in the loss and re-establishment of desiccation tolerance in germinated pea seeds
Authors: Sahu Balram , Naithani Subhash Chandra 
Published: October 2022

Abstract
This study highlights the regulatory function of ROS and its detoxifying enzymes during loss and re-establishment of desiccation tolerance (DT) in PEG pretreated and untreated germinated pea (Pisum sativum L.) seeds with 2 mm long radicles. Dehydration of untreated pea seedlings to initial water content (IWC; 0.11 gH2O g− 1 DM) and below IWC (0.06 gH2O g− 1 DM) reduced the DT to 60 and 20%, respectively. Significant amelioration in DT was observed in PEG pretreated seedlings by 100 and 70% following dehydration at IWC and below IWC, respectively. Rendering DT in the PEG pretreated seedlings was negatively associated with an extensive reduction in superoxide (~ 2.3 folds) and H2O2 (~ 1.8 folds) levels compared to the untreated seedlings. Enhanced expression, levels (quantitative) and number (isoenzyme) of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) in the PEG pretreated seedlings negatively correlated with the lowering ROS levels and its associated oxidative damage. It is concluded that the re-induction of increased antioxidative capacity is a key factor in the re-establishment of DT in PEG-pretreated pea seedlings. Additionally, PEG pretreatment resulted in 50% disappearance of superoxide in the sub-apical zone (SZ) of the radicle compared to the radicle of untreated seedlings dehydrated below IWC. Further, in situ detection of superoxide accumulation in the apical zone (AZ) was NADPH-oxidase dependent but in the SZ it was independent of NADPH-oxidase.
Head Coach Gender and Player Performance in NCAA Softball
Authors: Paulson Courney , Darvin Lindsey , Berri David 
Published: October 2022

Abstract
In most industry segments, more than 70% of leadership positions are held by men. This leads to the common suggestion that men are better leaders than women. Unfortunately, in most industries, it is difficult to find women and men in leadership positions where performance of the leader can be objectively evaluated. Hence, in most places, it is difficult to establish if gender identity of the leader makes a measurable difference. The exception to this general trend is in the world of sports. Both women and men do work as coaches in women’s sports, often in equivalent roles and positions. Consequently, we can scientifically evaluate if the gender of the coach impacts the outcomes we observe. In this article, we specifically consider the sport of college softball, where we note a more equal breakdown in coaching by gender than most other sports. The evidence from college softball indicates gender of the coach does not impact outcomes. Specifically, we find the gender of the head coach does not appear to alter the performance of individual hitters and thus a team’s offensive production. Such findings challenge the gender stereotypes we see in the broader leadership positions of society.
Non-equilibrium flow of dense inhomogeneous fluids in nano-channels
Authors: Shan Baochao , Ju Long , Su Wei , Guo Zhaoli , Zhang Yonghao 
Published: October 2022

Abstract
The Enskog-Vlasov equation provides a consistent description of the microscopic molecular interactions for real fluids based on the kinetic and mean-field theories. The present fluid flows in nano-channels are investigated by the Enskog-Vlasov-BGK model, which simplifies the complicated Enskog-Vlasov collision operator and enables large-scale engineering design simulations. The density distributions of real fluids are found to exhibit inhomogeneities across the nano-channel, particularly at large densities, as a direct consequence of the inhomogeneous force distributions caused by the real fluid effects including the fluid molecules' volume exclusion and the long-range molecular attraction. In contrast to the Navier-Stokes equation with the slip boundary condition, which fails to describe nano-scale flows due to the coexistence of confinement, non-equilibrium, and real fluid effects, the Enskog-Vlasov-BGK model is found to capture these effects accurately as confirmed by the corresponding molecular dynamics simulations for low and moderate fluid densities.
A Hybrid approach for Image Acquisition Methods based on Feature Based Image Registration (FBIR)
Authors: Kumawat Anchal , Panda Sucheta 
Published: October 2022

Abstract
In this paper, the problem of Feature-Based Image Registration (FBIR) has been addressed using a novel hybrid feature detector. To prove the efficiency of the proposed hybrid feature detector, its performance is compared with that of the state-of-the-art feature detectors like BRISK, FAST, ORB, Harris, MinEigen and MSER. Three different image acquisition methods are choosen for comparison. They are (i) Rotation transformation (ii) Scene to model transformation and (iii) Scaling transformation. Taking into consideration the above three types of image acquisition methods, a FBIR method is proposed for different types of remote sensing images. This paper proposed a hybrid feature detection algorithm, that consumes less time to detect the feature key points and it is also rotation and scale invariant. In addition to this the proposed algorithm performs well in terms of match points and match rate. This paper also focuses on a comparative analysis of BRISK, FAST, Harris, MinEigen, MSER and ORB with the proposed algorithm in terms of time to detect feature keypoints. It can be observed from the results and tables that in the case of hybrid feature detectors,It takes less time to detect the feature key points.
Up-regulation YTHDF1 and YTHDF3 is related to the prognosis of oral squamous cell carcinoma
Authors: Shi Rui , Li Shao-ming , Gao Ling , Zhi Ke-qian , ren wen-hao 
Published: October 2022

Abstract
Background
N6-methyladenosine (m6A) is the most abundant internal modification in mammalian mRNAs. M6A is composed of methyl-transferases, which act as ("writers"), binding proteins, which act as ("readers"), and demethylases, which act as ("erasers"). As an important part of ("readers"), YTHDF1 and YTHDF3 were shown to be associated with many cancers. This paper aimed to study the expression of YTHDF1 and YTHDF3 in oral squamous cell carcinoma (OSCC).
Methods
We detected the expression of YTHDF1 and YTHDF3 in 120 OSCC patients by immunohistochemical analysis. Statistical analysis was used to determine whether the high or low expression of these two genes was significantly associated with age, gender, histological type, clinical stage, or lymph node metastasis. The correlation curve and survival curve of the two genes were produced to evaluate the potential clinical significance.
Results
We find the expression of YTHDF1 and YTHDF3 was increased in OSCC tissues compared to adjacent normal tissues. The statistical analysis showed that the expression of YTHDF1 and YTHDF3 was significantly associated with the clinical stage and histological type in OSCC patients. There was also a significant correlation between the expression of YTHDF1 and YTHDF3. A high expression of YTHDF1 and YTHDF3 was related to poor patient prognosis.
Conclusions
Our findings suggest that a high expression of YTHDF1 and YTHDF3 may be related to poor patient prognosis.
Barriers to Rapid Triage in Trauma Centers: A Qualitative Content Analysis
Authors: Monsef-Kasmaei Bahare , Zohrevandi Behzad , Bakerdar Fatemeh Haghshenas , Kouchakinejad-Eramsadati Leila , Khodadadi-Hassankiadeh Naema 
Published: October 2022

Abstract

Background Although specialized trauma care in the triage unit can significantly increase the chances of survival of the injured, the obstacles to rapid triage are still a dilemma. Identifying these barriers is quite essential for effective clinical decision-making and improving positive outcomes. Objective This study aimed to report the experiences of the physicians and nurses regarding the obstacles to rapid triage. Method A qualitative study was performed on twenty physicians and nurses working in the triage unit of Poursina Hospital in northern Iran in 2021 using semi-structured interviews. The experiences were analyzed by the content analysis method. Credibility, reliability, and validity were used for data accuracy and trustworthiness. Sampling continued until data saturation. Results A total of 5 categories and 28 subcategories were obtained including the challenges related to employees, physical resource limitations, disruptions in the processes of triage, controlling and monitoring challenges, and specific characteristics of the referred patients. Conclusion Hospital authorities need to eliminate the barriers to rapid triage of patients which are related to education, experience, training, and retraining of triage staff. Physical resources in these units should increase and the triage processes need to improve by proper leveling of the patients and creating a triage view. The lack of coordination between the pre-hospital emergency team and the triage unit should be improved. Finally, the triage staff can strengthen the therapeutic interaction with agitated patients and caregivers.
Cell quantification in digital contrast microscopy images with Convolutional Neural Networks algorithm
Authors: Ferreira Eloiza K. G. D. , Lara Daniel S. D. , Silveira Guilherme F. 
Published: October 2022

Abstract
High Content Screening (HCS) combines high throughput techniques with the ability to generate cellular images of biological systems. The objective of this work is to evaluate the performance of predictive models using CNN to identify the number of cells present in digital contrast microscopy images obtained by HCS. One way to evaluate the algorithm was through the Mean Squared Error metric. The MSE was 4,335.99 in the A549 cell line, 25,295.23 in the Huh7 and 36,897.03 in the 3T3. After obtaining these values, different parameters of the models were changed to verify how they behave. By reducing the number of images, the MSE increased considerably, with the A549 cell line changing to 49,973.52, Huh7 to 79,473.88 and 3T3 to 52,977.05. Correlation analyzes were performed for the different models. In lineage A549, the best model showed a positive correlation with R = 0.953. In Huh7, the best correlation of the model was R = 0.821, it was also a positive correlation. In 3T3, the models showed no correlation, with the best model having R = 0.100. The models performed well in quantifying the number of cells, and the number and quality of the images interfered with this predictive ability.
Human Herpesvirus 7 encephalitis mainfests with epileptic seizures in an immunocompetent adult and review of the literature
Authors: Li Yi , Deng Qiu Ming 
Published: October 2022

Abstract
Introduction: Human Herpesvirus 7(HHV-7)is a widespread double-strand DNA virus in human population that belongs to β-herpesvirinae subfamily and it replicates in CD4 + T lymphocytes. The virus has the ability of lifelong latency and easy to reactivate in immunocompromised patients(1.2). Primary HHV-7 infection regularly occurs during childhood and may mainfests several clinical symptoms, mainly fever and exanthem subitum. Furthermore, HHV-7 Encephalitis is the severely type of the infection, epileptic seizures belongs to its clinical manifestation, however there exist uncertain aspects about infection route except the past history of the infectious agent in patients. In particular, as a lymphotropic virus, it has obvious tropism for the central nervous system(CNS), but its neuropathogenesis are underestimated(3), one the other hand, the case of encephalitis associated with human herpesvirus-7 infection in an immunocompetent patient has been rarely reported. Thus, we mainfest an immunocompetent 26-year-old woman with encephalitis that the infection reason is not clear and review current literature. With the treatment of acyclovir and ganciclovir. The clinical outcome in our patient was recovered.
Behavioral Characteristics of Pediatric Surgery Patients and Their Parents and Treatment Planning During the Closure Period of the COVID 19 Pandemic
Authors: Ayvaz Olga Devrim , Celayir Ayşenur , Pehlivan Erkan 
Published: October 2022

Abstract
Background
We aimed to determine the behavioral attitudes and diagnostic/interventional characteristics of the patients relatives who applied to our polyclinic during the closure period of COVID 19 pandemic.
Methods
This prospective study was conducted between June-July 2020. The data were statistically analyzed.
Results
Hundred and five(61.8%) male, 65(38.2%) female patients who applied to our outpatient clinic. The median age was 2 years. A total of children came with their parents, and 99.4% were wearing masks. Although 93.5% of the parents were wearing their masks correctly. At least one of the additional protective outfits was used by 7.1% parents. Fourty(23.5%) parents adapted at least one of the health protective behaviors. 52.4% patients were not wearing masks; and 92.6% masked people were wearing them correctly. Surgery was performed in 10.5% of the patients under general anesthesia.
Conclusion
The use of masks by the children of working mothers and older fathers, the correct use of the masks by the patient influenced by the father's profession, decreased use of additional health protective methods by those who came by their own vehicles, increase in the frequency of wearing masks as the child got older were found to be statistically significant parameters.
Application of Newly Synthesized Fe 3 O 4 @MPTMS-Dithizone Magnetic Nanoparticles for Sensitive Analysis of Sibutramine Molecules in Herbal Slimming Products
Authors: Demir Özge , Ulusoy Halil İbrahim , Polat Ümmügülsüm , Ulusoy Songül 
Published: October 2022

Abstract
A new enrichment and sensitive determination method, which includes HPLC-DAD analysis after Magnetic Based Solid Phase Extraction (MSPE), has been developed for trace analysis of Sibutramine molecules in herbal slimming tea samples. In the proposed method, Sibutramine molecules were pre-concentrated by using a newly synthetized magnetic sorbent in our laboratory. Desorption of Sibutramine molecules from sorbent phase were carried out by using acetonitrile/methanol (1:1) solvent in the presence of pH 8.0 buffer before chromatographic determinations. Analytical parameters of method such as linear range, enrichment factor, and determination limit were determined after optimizing experimental variables such as interaction time, desorption solvent, pH, etc. Sibutramine molecule was analyzed by isocratic elution of acetonitrile and KH2PO4 (pH 3.0, 0.05 M) (40:60) with DAD detector at 223 nm wavelength. Limit of detection (LOD) value was calculated as 1.43 ng mL− 1. Relative standard deviations (RSD) were below 3.20% for determinations of model solutions including 200 ng mL− 1 of Sibutramine. Finally, the developed method has been applied to herbal slimming tea samples with quantitative in recovery experiments.
Comparison of neonatal outcomes between multiples and singletons among very low birth weight infants: The Korean Neonatal Network Cohort Study
Authors: Jang Hyeon Gu , Choi Seoheui , Noh O Kyu , Hwang Jong Hee , Lee Jang Hoon 
Published: October 2022

Abstract
To compare neonatal outcomes between multiples and singletons among very low birth weight infants (VLBWIs), this was a prospective cohort study that was conducted by collecting data registered in the Korean Neonatal Network (KNN) database from January 2013 to December 2016. There were 8265 infants in the KNN database, and 2958 of them were from a multiple pregnancy (MP VLBWIs). Among them, 2636 infants were twins, 308 infants were triplets, and 14 infants were quadruplets. MP VLBWIs had a higher gestational age, birth weight, Apgar scores at 5 minutes, the rates of caesarean section and artificial reproductive technology (ART) but lower maternal hypertension, oligohydramnios, chorioamnionitis rates and CRIB scores than the singleton VLBWIs (SG VLBWIs). In univariate analysis, the MP VLBWIs had a lower incidence of respiratory distress syndrome, bronchopulmonary dysplasia, and sepsis, but the mortality rate was not significantly different for overall gestational ages except born at ≤ 26 weeks of gestation. In multivariate logistic analysis, the incidences of intraventricular haemorrhage (grade ≥ 3), and retinopathy of prematurity requiring treatment were significantly higher than the SG VLBWIs. A new strategy to improve the mortality of immature MP VLBWIs born at ≤ 26 weeks of gestation should be developed.
Genomic prediction of agronomic traits in sugarcane using machine learning and quantitative genetics
Authors: Inamori Minoru , Kimura Tatsuro , Mori Masaaki , Tarumoto Yusuke , Hattori Taiichiro , Hayano Michiko , Umeda Makoto , Iwata Hiroyoshi 
Published: October 2022

Abstract
To improve the efficiency of sugarcane breeding, introduction of genomic selection (GS) into breeding programs is anticipated. For sugarcane cultivars with highly heterozygous polyploid genomes, non-additive genetic effects must be considered in genomic prediction models. In this context, the use of machine learning techniques is an effective approach to model nonadditive genetic effects. In addition, pedigree information that tracks sugarcane breeding lineages can be used to calculate the degree of association among genotypes and as input for predictive models. In the present study, we used the breeding population used in a KARC/NARO sugarcane breeding program in Japan as experimental material and verified the following attributes. Specifically, the prediction accuracy of BLUP was validated based on genomic and pedigree information. In addition, a new machine learning method that can explicitly process the interaction between whole-genome markers and genetic background was developed, and comparative verification was performed using the conventional random forest method. Pedigree information showed high predictive accuracy, and when columns of the numerator relationship matrices were used as input for the machine learning models, their accuracy was identical or superior to that of BLUP depending on traits. Finally, we confirmed the effectiveness of the proposed machine learning method using interactions among markers as a model. Overall, these GS methods are expected to improve sugarcane breeding efficiency.
Linked color imaging combined with Kyoto Classification of Gastritis to establish a training model for general practitioners with a special interest in digestive diseases: a quantitative study of general practitioner training in China
Authors: Huang Jun , Xue Lan-feng , Huang Qing-ju , Chen Zhen-yu , Qiao Wei-guang , Chen Yu 
Published: October 2022

Abstract
Background
Linked color imaging (LCI) can improve the diagnostic rate of Helicobacter pylori (H. pylori) infection-related gastritis, and Kyoto Classification of Gastritis contributes to the diagnosis of H. pylori infection under endoscopy. The present study aims to combine LCI with Kyoto Classification of Gastritis to establish a training model for general practitioners (GPs) with a special interest in digestive diseases.
Methods
Gastroenterology sub-specialty training was conducted for GPs. After analysis by expert endoscopists, a simplified Kyoto Classification of Gastritis was generated to prepare the questionnaire. The questionnaire containing images in white light imaging (WLI) mode and LCI mode was used to assess the trainees' ability to determine the status of H. pylori infection by observing endoscopic mucosal manifestations before and after learning the Kyoto Classification of Gastritis. SPSS 26.0 software was used for statistical analysis.
Results
The analysis of expert endoscopists on the Kyoto Classification of Gastritis showed that gastroscopic mucosal manifestations including mucosal atrophy, hematin, mucosal swelling, and sticky mucus had an impact on the judgment of H. pylori infection (P < 0.05). After training, the total questionnaire score of GPs was improved (3.48 vs 4.45, P < 0.05), and there was no difference in the questionnaire completion time between GPs and standard gastroenterologists (SGs). After training, the questionnaire score of GPs based on images in WLI and LCI modes was improved. The score of the LCI mode was higher than that of the WLI mode (LCI: 1.45 vs 2.14, P < 0.05, WLI: 2.04 vs 2.31, P = 0.355).
Conclusions
Kyoto Classification of Gastritis combined with LCI improves the ability of GPs to diagnose H. pylori infection through endoscopic images.
Persistent T cell-mediated immune responses against Omicron variants after the third COVID-19 mRNA vaccine dose
Authors: Belik Milja , Liedes Oona , Vara Saimi , Haveri Anu , Pöysti Sakari , Kolehmainen Pekka , Maljanen Sari , Huttunen Moona , Reinholm Arttu , Lundberg Rickard , Skön Marika , Osterlund Pamela , Melin Merit , Hänninen Arno , Hurme Antti , Ivaska Lauri , Tähtinen Paula , Lempainen Johanna , Kakkola Laura , Jalkanen Pinja , Julkunen Ilkka 
Published: October 2022

Abstract
The prime-boost COVID-19 mRNA vaccination strategy has proven to be effective against severe COVID-19 disease and death. However, concerns have been raised due to decreasing neutralizing antibody levels after COVID-19 vaccination and due to the emergence of new immuno-evasive SARS-CoV-2 variants that may require additional booster vaccinations. Here we show that within the vaccinated health care workers (HCWs) the third mRNA vaccine dose recalls both humoral and T cell-mediated immune responses and induces high levels of neutralizing antibodies against Omicron BA.1 and BA.2 variants. Three weeks after the third vaccine dose, SARS-CoV-2 wild type spike protein-specific CD4+ and CD8+ T cells are observed in 82% and 71% of HCWs, respectively, and the T cells cross-recognize both Omicron BA.1 and BA.2 spike peptides. Although the levels of neutralizing antibodies against Omicron BA.1 and BA.2 decline 2.5 to 3.8-fold three months after the third dose, Th1-type memory CD4+ T cell responses are maintained for at least 7 months post the second dose and 3 months post the third vaccine dose suggesting durable immune protection.
Taxonomic and phylogenetic shifts following an inland derecho
Authors: Barfknecht David F. , Thapa Saroj , Gibson David J. , Holzmueller Eric J. 
Published: October 2022

Abstract
In 2009, a derecho occurred in southern Illinois affecting large areas of the regional forest systems. Previous research reported multi-strata species compositional shifts post-derecho at LaRue Pine Hills/Otter Pond Research Natural Area (LPH/OP RNA). Here, we expand these observations to include phylogenetic and spatial relationships, including elevation as a covariate. Surveys were conducted before and after the derecho. Univariate and multivariate analyses documented diversity and compositional shifts. Spatial analyses identified distinct spatial patterns in taxonomic (TSR) and phylogenetic (PSR) species richness, and metrics of phylogenetic clustering and overdispersion (net relatedness index and nearest taxon index; NRI and NTI, respectively), in seedling, sapling, and tree communities. Decreases in tree TSR and PSR and sapling PSR, and increases in sapling NTI occurred post-derecho. Seedling diversity-elevational relationships exhibited a humped-backed relationship in all cases excluding NTI pre-derecho, while saplings showed positive linear relationships between phylogenetic metrics and elevation. Despite the relatively small elevation gradient (122m), multivariate analyses identified elevation as the most influential environmental driver of community dissimilarity. Local Moran’s I analysis showed post-derecho clustering in the north-central extent. Co-Kriging (CoK) interpolation predicted that seedling diversity increased in the southwestern extent, while sapling diversity increased in the eastern extent and tree diversity increased from the north-central extent post-derecho, indicating competitive interactions at lower elevations and environmental filtering at higher elevations. Seedling and tree NRI and NTI increased in the southern extent, whereas sapling clustering increased in the northeastern extent. Co-Kriging had a better prediction capability compared to other spatial interpolation methods.