bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative transcriptome analysis unravels key factors regulating nitrogen use efficiency in chrysanthemum
Authors: GE Lijiao , MIAO Weihao , DUAN Kuolin , SUN Tong , Fang Xinyan , GUAN Zhiyong , JIANG Jiafu , CHEN Sumei , FANG Weimin , CHEN Fadi , ZHAO Shuang 
Published: November 2022

Abstract
Background: Nitrogen (N) is a limiting factor that determines yield and quality of chrysanthemum. Genetic variation in N use efficiency (NUE) has been reported among genotypes of chrysanthemum. We performed transcriptome analysis of two chrysanthemum genotypes, ‘Nannonglihuang’ (‘LH’, N-efficient genotype) and ‘Nannongxuefeng’ (‘XF’, N-inefficient genotype), under low N (0.4 mM/L N) and normal N (8 mM/L N) treatments for 15 d and N recovery treatment for 12 h (low N treatment for 15 d and then normal N treatment for 12 h) to understand the genetic factors impacting NUE in chrysanthemum.
Results: The two genotypes exhibited contrasting responses to different N treatments. N-efficient genotype ‘LH’had significant superiority in agronomic traits, N accumulation and glutamine synthase (GS) activity, under normal N and low N treatments. Transcriptome analysis revealed that low N treatment promoted root growth and increased the expression of partial N uptake genes, genes related to auxin, abscisic acid, and gibberellin signal transduction in roots of ‘LH’. N recovery treatment increased the expression of genes related to the cytokinin signal transduction in roots of ‘LH’. The expression of NRT2-1, AMT1-1, Gln1, genes related to gibberellin (GID1B, GID2, GAI) and cytokinin signal transduction (AHK2, AHK3, AHK4, ARR4, ARR5, ARR9) in roots of ‘LH’ was higher than that in ‘XF’ under different N treatments, suggesting that the genes related to N metabolism and hormone (auxin, abscisic acid, gibberellin, and cytokinin) signal transduction in roots of ‘LH’ were more sensitive to different N treatments than those of ‘XF’. The network analysis also identified hub genes like bZIP43, bHLH93, NPF6.3, IBR10, MYB62, PP2C, PP2C06 and NLP7, which may be the key regulators of N-mediated response in chrysanthemum and play crucial roles in enhancing NUE and resistance to low N stress in the N-efficient chrysanthemum genotype.
Conclusion: This study unravels key factors of regulating NUE in chrysanthemum at the genetic level, and could be useful for genotype improvement and breeding of high NUE chrysanthemum genotypes.
Impact of Long Term Cyclamate and Saccharin Consumption on Biochemical Parameters in Healthy Individuals and Type 2 Diabetes Mellitus Patients
Authors: Hasan Husni Muhammed , Alkass Suad Yousif , de Oliveira Daniele Suzete Persike 
Published: November 2022

Abstract
Previous studies on saccharin and cyclamate were either limited to experimental animals or lacking evaluation of their long-term consumption effects in humans. This study evaluated the effect of chronic consumption of saccharin and cyclamate on biochemical parameters in healthy individuals and patients with type 2 diabetes mellitus. Healthy and diabetic individuals were classified into two groups based on whether they consumed sweeteners or not. The participants were classified according to the amount of sweetener consumed per day and duration of consumption. Serum catalase activity, peroxynitrite, ceruloplasmin and malondialdehyde concentrations were determined. Glycated hemoglobin, fasting glucose, creatinine, alanine transaminase and lipid profile were also evaluated. Saccharin and cyclamate increased HbA1C (+ 11.16%), MDA (+ 52.38%), TG (+ 16.74%), LDL (+ 13.39%) and TC/HDL (+ 13.11%) in healthy volunteers. Diabetic patients consuming sweetener showed increased FSG (+ 17.51%), ceruloplasmin (+ 13.17%) and MDA (+ 8.92%). Diabetic patients showed positive correlation between the number of tablets consumed per day with FSG and serum creatinine. Positive correlation was found between the duration of sweetener consumption and FSG as well as TG. consumption of saccharin and cyclamate affected biochemical parameters related to metabolic functions in a time and dose dependent manner and appear to increase oxidative stress in healthy and diabetic type 2 patients.
‘Our Bodies are Not Strong Anymore’: A Focus Group Study of Health Risk Perception of Ambient Air Pollution Near a Petrochemical Industry.
Authors: Okoye Ogochukwu , Carnegie Elaine , Luca Mora 
Published: November 2022

Abstract

Background
Ambient air pollution has persisted in many communities resulting in chronic human exposure to unhealthy pollutants. Epidemiological studies on air pollution have been mainly quantitative with a dearth of information on health risk perception, a key component of environmental health risk management. The aim of this qualitative study was to illuminate the health risk perception of ambient air pollution amongst persons residing near a petrochemical industry. In addition, determine their perception of existing control measures and ideas for more effective control.
Methods
A focus group study of residents near a petrochemical refinery. Participants were purposively selected based on age, sex, long-term residence in the community, occupation, and involvement with community leadership. Three 90mins face-to-face focus groups and one individual interview were conducted while observing COVID-19 safety precautions. The moderator guided discussions using a pre-formed topic guide. All discussions were audio-recorded, transcribed manually and coded using NVivo soft-ware. Data analysis was conducted using reflective thematic analysis proposed by Braun and Clarke.
Results
Twenty-four adults agreed to participate; 23 in three focus groups and one individual interview. Six themes were inductively generated from the data as follows: Negative perception of the environment, the refinery is to blame, Air pollution is seen or smelt, Air pollution is associated with health and non-health risks, Poor response to air pollution: everyone is to blame, and Government is primarily responsible for healthy air quality. The participants perceived their ambient air was polluted mainly by activities of petrochemical industries, but were not aware of the extent of its potential health implications. Key suggestions for air pollution control included stopping gas flaring, regulating tanker drivers and illegal artisanal refineries, environmental health education, dissemination of research findings to stakeholders, access to affordable health care and providing incentives for community members
Conclusions Participants perceive their ambient air was unhealthy, however concern about the health risks was shaped by demographic, socio-economic and other contextual factors. Existing mitigating efforts were perceived as inadequate and the key barriers were poor environmental health literacy and political factors. More inclusive and collaborative approaches are required for improved environmental and public health.
Helium administration is more effective during piston-driven high-frequency oscillatory ventilation than conventional mechanical ventilation in a rabbit model
Authors: Akiyama Louis , Tatsunami Shinobu , Akita Mieko , Shimizu Naoki 
Published: November 2022

Abstract
Background
Low-molecular-weight gases such as helium are more likely to form laminar flows. Using helium-oxygen mixtures instead of nitrogen-oxygen mixtures during mechanical ventilation has been shown to accelerate carbon dioxide (CO2) excretion. Helium-oxygen mixtures have been used with mechanical ventilator settings such as high-frequency oscillatory ventilation (HFOV) and conventional mechanical ventilation (CMV). However, to the best of our knowledge, studies have not been conducted to determine the optimal ventilator setting for the promotion of CO2 excretion when helium is administered. This study aimed to compare the efficacy of using helium-oxygen gas mixtures between pressure-controlled CMV and piston-driven HFOV in rabbit models. CO2 transition was evaluated using blood gas analysis performed during and after helium administration. Experimental data were analyzed using parametric statistical methods. A repeated measures analysis of variance was used to compare CO2 excretion between HFOV and CMV.
Results
There was no statistically significant difference in the partial pressure of CO2 (PaCO2) or oxygen (PaO2) values before or after helium administration. Compared with pre-helium administration, PaCO2 values during helium administration were decreased under both CMV and piston-driven HFOV. Moreover, the PaCO2 transition during helium administration while using piston-driven HFOV was shown to be statistically significantly different (P < 0.001).
Conclusions
This study demonstrated that helium enhances CO2 elimination more significantly during piston-driven HFOV compared with that in CMV in rabbit models with normal lungs. With further study and clinical trials, these results imply that the use of HFOV while using a helium-oxygen mixture may improve CO2 excretion.
Untangling the inhibitory effect of dust aerosols on weak precipitation
Authors: Wang Jinyan , Xie Xiangshan , Ding Ruiqiang , Yasheng Dilinuer , Wang Tianyu , Sun Caixia , wang Zhiyi 
Published: November 2022

Abstract
As one of the most abundant and widely distributed aerosols in the troposphere, dust aerosols act as condensation nucleus to alter the life cycle of clouds and precipitation, thus affecting weather and climate. However, the effects of dust aerosols on precipitation are still not fully understood, particularly for the raindrop size distribution (DSD) of weak precipitation and thus warrant investigations. Taking multiple precipitation episodes occurring from March 15th to April 1st, 2021 in Pudong, Shanghai, China as an example, we attempt to reveal the underlying impacts of dust aerosols on weak precipitation’s DSD. Compared with the weak precipitation events with and without dust aerosols, we find that dust aerosols may inhibit weak precipitation. In details, the mean mass weighted average diameter (Dm) of dust-carrying precipitation is much smaller than the weak precipitation without dust aerosols, while the mean value of normalized intercept parameters (Log10Nw) of dust carrying precipitation is greater than the dust-free precipitation. Moreover, the dust-carrying precipitation episodes have higher (lower) number concentration of small (large) raindrops than the precipitation events without dust aerosols, demonstrating that dust aerosols could lead to a significant increase (decrease) in the number concentration of small (large) raindrops. As a results, dust aerosols are favorable to stimulate a more concentrated distribution of raindrop size and a smaller spectral width, prolonging the life of clouds and thus inhibiting weak precipitation. These findings highlight the inhibitory effect of dust aerosols on weak precipitation and can provide scientific insights for weather and climate prediction.
DSS For Personal Financial Planning
Authors: Kannammal A , Harini C , Roshini G 
Published: November 2022

Abstract
Personal financial planning is an essential tool for people over various age groups that assists people in planning their financial needs for now and later as years move on. The planning includes segregation of money into various activities that might include daily expenses, emergency expenses and savings. For an employee who plans to save for the future it becomes necessary to plan his daily expenditure, and select an appropriate investment strategy which will in turn decrease financial risk in later ages of retirement. Financial management professionals help in managing funds into controlled transactions which will later provide the investor a greater financial support and return over years. It is not always efficient and reliable to find professionals who provide financial solutions with sound knowledge on returns through the investment analyzing the risk involved, to make financial planning a more reliable and easy approach, using softwares are the better ways. Through a software or analytical based approach it is always possible to ensure that there are sufficient funds available for individuals at any stage of life. Financial management tools help individuals cultivate a habit of budgeting their expenses and avoid unnecessary spending, also, they tend to prioritize tier spending patterns. This also helps to identify the full cashflow for both monthly and yearly expenses and saving then this plan recommends some investment plan from the range of risk-taking score for each individual.
Bifurcation analysis for a diffusive predator-prey system with spatial heterogeneity and delays
Authors: Zhang Hua , Wei Junjie 
Published: November 2022

Abstract
In this paper, we consider a diffusive Lotka-Volterra predator-prey system with spatial heterogeneity and two delays. We first show that there exists a nonconstant positive steady state when the diffusion rates of prey and predator are large. Then we obtain the stability of the steady state and show the existence of a Hopf bifurcation. Moreover, some numerical simulations are provided to illustrate our theoretical results.
MSC 2010: 35K57, 37G15, 37N25, 92D25
Single-cell RNA sequencing and molecular docking reveal sinomenine can treat postherpetic neuralgia by regulating the expression of MMP2
Authors: Zhang Hongli , Zhao Yong 
Published: November 2022

Abstract
Objective: The pathogenesis of postherpetic neuralgia is not clear, the pain is severe, the duration is long, and the treatment effect is poor. We explored the potential mechanism of sinomenine in the treatment of postherpetic neuralgia based on single-cell sequencing and molecular docking technology and provided a theoretical basis for studying the pathogenesis of postherpetic neuralgia.
Methods: Based on single-cell sequencing analysis and bioinformatics, the possible pain-causing factors of postherpetic neuralgia were identified, and the interaction between sinomenine and the target protein was analyzed using the molecular docking technique. First, the genes related to sinomenine and postherpetic neuralgia were found through various databases. Then, single-cell gene sequencing was used to identify pain-related marker genes and their role in cell subsets. The intersection of the differentially expressed (|LogFC|>1) marker gene and the postherpetic neuralgia-related genes in single-cell sequencing was used as the postherpetic neuralgia pain-causing gene set. Intersection of sinomenine and hub genes was used to identify the target genes that interact with each other, and sinomenine was molecularly docked with the target genes.
Results: A total of 2524 postherpetic neuralgia-related genes and 43 sinomenine-related genes were identified through Genecard, CTD, and the GEO database. Single-cell gene sequencing was performed and revealed a total of 680 marker genes between pain and pain-free cell populations |logFC|>1. Pain-associated subpopulations were annotated mainly with microglia and astrocytes. And there were 125 intersection genes associated with postherpetic neuralgia. These genes were associated with the development of postherpetic neuralgia. 42 hub gene were identified from these crossover genes. MMP2 is the common gene of hub genes and sinomenine-related genes. Molecular docking discovers better affinity and stability between sinomenine and MMP2 protein.
Conclusion: This study combined single cell analysis, bioinformatics and molecular docking to identify MMP2 as a potential target for sinomenine in the treatment of postherpetic neuralgia, which may act on microglia to alleviate pain by regulating the neuroinflammatory response and ion pathways.
Clinical outcomes of sodium bicarbonate therapy in critically ill pediatric patients with metabolic acidosis are associated with chloride
Authors: Liu Huaqing , Cao Yanmei , Xue Xiaoyan , Hong Yi , Lu Meihua , Bai Zhenjiang , Wu Shuiyan 
Published: November 2022

Abstract
Background: Metabolic acidosis is a common acid-base imbalance in critically ill patients. Whether sodium bicarbonate (SB) can improve clinical outcomes in the treatment of metabolic acidosis is still controversial. The aim of this study was to determine the factors influencing the clinical efficacy of SB in the treatment of metabolic acidosis and the potential benefit to patients.
Methods: Patients with metabolic acidosis who were treated with or without SB were identified and grouped from a retrospective cohort (Pediatric Intensive Care Unit [PICU] database), from which the clinical data were extracted. The in-hospital mortality curves of the acid-base balance parameters of patients in the two groups were drawn and fitted using the locally-weighted scatter plot smoothing (LOWESS) method. The prevalence ratios (PRs) of in-hospital mortality were estimated by log-binomial regression based on the maximum likelihood method, and the potential confounders, such as age and disease category, were adjusted.
Results: A total of 6,167 children with metabolic acidosis were enrolled, of whom 2,626 (42.58%) were treated with SB. The overall analysis showed that there was no significant difference in the in-hospital mortality rates (9.71% vs. 10.56%, p = 0.275) between children in the SB treatment and non-treatment groups, adjusted PR = 0.929 (95% CI, 0.802-1.072). There was no significant difference in the in-hospital mortality rates as a function of pH and HCO3- between the two groups. The in-hospital mortality rate as a function of chloride was significantly different; specifically, the curve of the untreated group was U-shaped and the curve of the treated group was L-shaped. The curves of the two groups crossed at 110 mmol/L of chloride after LOWESS fitting. There was no statistically significant difference in the risk of death between the SB treatment and non-treatment groups at a chloride < 107 mmol/L and a chloride >113 mmol/L. In the chloride < 107 mmol/L subgroup, SB treatment had a 41.7% increased risk of in-hospital death (adjusted PR=1.417, 95% CI, 1.069−1.481) and a 35.9% increased risk of 28-day death (adjusted PR=1.359, 95% CI, 1.315−1.474). In the chloride≥113 mmol/L subgroup, SB treatment had a 61.1% reduced risk of in-hospital death (adjusted PR=0.389, 95% CI, 0.268−0.553) and a 56.4% reduced risk of 28-day death (adjusted PR=0.436, 95% CI, 0.295−0.631). The median length of stay in the PICU of children in the SB group was also shorter than children in the non-treatment group when the chloride concentration was ≥110 mmol/L.
Conclusions: The clinical outcomes of SB in the treatment of metabolic acidosis are associated with chloride. When the chloride concentration was high (> 110 mmol/L), children benefited from SB treatment and when the chloride concentration was low (< 107 mmol/L), the risk of death increased.
Criticality in transient behavior of coupled oscillator system towards Chimera and Synchronization
Authors: Yao Nan , Zhang Qianyun , Ren Deyi , Li You-Jun , Su Chun-Wang , Huang Zi-Gang 
Published: November 2022

Abstract
Chimera states in spatiotemporal dynamical systems have been investigated in physical, chemical, and biological systems. While how the system is steering towards different final destinies upon spatially localized perturbation is still unknown. Through systematic numerical analysis of the evolution of the spatiotemporal pattern of the multi-cluster chimera states, we uncover a critical behavior of the system in transient time towards either chimera or synchronization as the final stable state. Based on adequate verification, we fit and analyze the distribution of the transient time, and observe power-law variation process as perturbation strengths approaches the critical point, which divided the phase of stable chimera and synchronization states. Moreover, the comparison between different clusters systems shows that the critical points of odd and even clusters converge in proportional like sequences as the cluster number approaches infinity, implying that this critical behavior can be modeled and enabling the articulation of a phenomenological model. Our work explores the criticality in transient behavior as well as the phase transition from chimera to synchronization under increasing perturbation, which may shed new insights to controlling coupled oscillator system with the help of perturbation through inducing phase transition between symmetry and asymmetry self-organized states.
Poor Self-Care Practices and Associated Factors among Type 2 Adult Diabetic Patients on Follow-Up Care at Public Health Facilities of Adama Town, Oromia, Ethiopia
Authors: Deybasso Haji Aman , Bekele Nardos Tilahun , Habtewold Ephrem Menikullih 
Published: November 2022

AbstractBackground Self-care practice plays a significant role in the reduction of life-threatening conditions. The study assessed the magnitude of poor self-care practices and associated factors among type 2 adult diabetic patients in Adama town in Ethiopia. Methods Institution-based cross-sectional study was conducted among 404 patients. Self-care practice was assessed by the summary of diabetes self-care activities questionnaires. Binary logistic regression was used to identify factors associated with poor self-care practices. Adjusted odds ratio with a 95% confidence interval was used to assess the strength of associations. The statistical significance was declared for a p-value < 0.05. Results The magnitude of poor self-care practices was 54% [95%CI: 49.1, 58.6]. Being divorced (AOR = 3.5; 95% CI: 1.0, 12.2), lower level of knowledge (AOR = 1.70; 95% CI: 1.0, 2.8), being on insulin (AOR = 6.3; 95% CI: 1.9, 20.6), taking oral medication (AOR = 8.6; 95% CI: 3.0, 24.5), unaware of fasting blood sugar (AOR = 2.9; 95% CI: 1.6, 5.2), not a member of diabetic association (AOR = 3.6; 95%CI: 1.7, 7.5), lack frequent social support (AOR = 2.9; 95% CI: 1.7, 4.9), and poor perceived benefit of self-care practices (AOR = 1.84; 95% CI: 1.0, 3.2) were associated with poor self-care practices. Conclusion The magnitude of poor self-care practices was higher than the national finding. Marital status, knowledge of diabetes and the level of fasting blood sugar, social support, types of medications, perceived benefit of self-care practices, and membership in the diabetic associations were independent determinants of poor self-care practices.