bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electronic cigarettes versus nicotine patches for smoking cessation in pregnancy: a randomized controlled trial
Authors: Hajek Peter , Przulj Dunja , Pesola Francesca , Griffiths Chris , Walton Robert , McRobbie Hayden , Coleman Tim , Lewis Sarah , Whitemore Rachel , Clark Miranda , Ussher Michael , Sinclair Lesley , Seager Emily , Cooper Sue , Bauld Linda , Naughton Felix , Sasieni Peter , Manyonda Isaac , Myers Smith Katie 
Published: May 2022

AbstractNicotine replacement therapy, in the form of nicotine patches, is commonly offered to pregnant women who smoke to help them to stop smoking, but this approach has limited efficacy in this population. Electronic cigarettes (e-cigarettes) are also used by pregnant women who smoke but their safety and efficacy in pregnancy are unknown. Here, we report the results of a randomized controlled trial in 1,140 participants comparing refillable e-cigarettes with nicotine patches. Pregnant women who smoked were randomized to e-cigarettes (n = 569) or nicotine patches (n = 571). In the unadjusted analysis of the primary outcome, validated prolonged quit rates at the end of pregnancy in the two study arms were not significantly different (6.8% versus 4.4% in the e-cigarette and patch arms, respectively; relative risk (RR) = 1.55, 95%CI: 0.95–2.53, P = 0.08). However, some participants in the nicotine patch group also used e-cigarettes during the study. In a pre-specified sensitivity analysis excluding abstinent participants who used non-allocated products, e-cigarettes were more effective than patches (6.8% versus 3.6%; RR = 1.93, 95%CI: 1.14–3.26, P = 0.02). Safety outcomes included adverse events and maternal and birth outcomes. The safety profile was found to be similar for both study products, however, low birthweight (<2,500 g) was less frequent in the e-cigarette arm (14.8% versus 9.6%; RR = 0.65, 95%CI: 0.47–0.90, P = 0.01). Other adverse events and birth outcomes were similar in the two study arms. E-cigarettes might help women who are pregnant to stop smoking, and their safety for use in pregnancy is similar to that of nicotine patches. ISRCTN62025374.
Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models
Authors: Bates Mark , Keller-Findeisen Jan , Przybylski Adrian , Hüper Andreas , Stephan Till , Ilgen Peter , Cereceda Delgado Angel R. , D’Este Elisa , Egner Alexander , Jakobs Stefan , Sahl Steffen J. , Hell Stefan W. 
Published: May 2022

AbstractCoherent fluorescence imaging with two objective lenses (4Pi detection) enables single-molecule localization microscopy with sub-10 nm spatial resolution in three dimensions. Despite its outstanding sensitivity, wider application of this technique has been hindered by complex instrumentation and the challenging nature of the data analysis. Here we report the development of a 4Pi-STORM microscope, which obtains optimal resolution and accuracy by modeling the 4Pi point spread function (PSF) dynamically while also using a simpler optical design. Dynamic spline PSF models incorporate fluctuations in the modulation phase of the experimentally determined PSF, capturing the temporal evolution of the optical system. Our method reaches the theoretical limits for precision and minimizes phase-wrapping artifacts by making full use of the information content of the data. 4Pi-STORM achieves a near-isotropic three-dimensional localization precision of 2–3 nm, and we demonstrate its capabilities by investigating protein and nucleic acid organization in primary neurons and mammalian mitochondria.
Cryo-EM structure of an active central apparatus
Authors: Han Long , Rao Qinhui , Yang Renbin , Wang Yue , Chai Pengxin , Xiong Yong , Zhang Kai 
Published: May 2022

AbstractAccurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia.
A global 0.05° dataset for gross primary production of sunlit and shaded vegetation canopies from 1992 to 2020
Authors: Bi Wenjun , He Wei , Zhou Yanlian , Ju Weimin , Liu Yibo , Liu Yang , Zhang Xiaoyu , Wei Xiaonan , Cheng Nuo 
Published: May 2022

AbstractDistinguishing gross primary production of sunlit and shaded leaves (GPPsun and GPPshade) is crucial for improving our understanding of the underlying mechanisms regulating long-term GPP variations. Here we produce a global 0.05°, 8-day dataset for GPP, GPPshade and GPPsun over 1992–2020 using an updated two-leaf light use efficiency model (TL-LUE), which is driven by the GLOBMAP leaf area index, CRUJRA meteorology, and ESA-CCI land cover. Our products estimate the mean annual totals of global GPP, GPPsun, and GPPshade over 1992–2020 at 125.0 ± 3.8 (mean ± std) Pg C a−1, 50.5 ± 1.2 Pg C a−1, and 74.5 ± 2.6 Pg C a−1, respectively, in which EBF (evergreen broadleaf forest) and CRO (crops) contribute more than half of the totals. They show clear increasing trends over time, in which the trend of GPP (also GPPsun and GPPshade) for CRO is distinctively greatest, and that for DBF (deciduous broadleaf forest) is relatively large and GPPshade overwhelmingly outweighs GPPsun. This new dataset advances our in-depth understanding of large-scale carbon cycle processes and dynamics.
Multiscale mechanical consequences of ocean acidification for cold-water corals
Authors: Wolfram Uwe , Peña Fernández Marta , McPhee Samuel , Smith Ewan , Beck Rainer J. , Shephard Jonathan D. , Ozel Ali , Erskine Craig S. , Büscher Janina , Titschack Jürgen , Roberts J. Murray , Hennige Sebastian J. 
Published: May 2022

AbstractOcean acidification is a threat to deep-sea corals and could lead to dramatic and rapid loss of the reef framework habitat they build. Weakening of structurally critical parts of the coral reef framework can lead to physical habitat collapse on an ecosystem scale, reducing the potential for biodiversity support. The mechanism underpinning crumbling and collapse of corals can be described via a combination of laboratory-scale experiments and mathematical and computational models. We synthesise data from electron back-scatter diffraction, micro-computed tomography, and micromechanical experiments, supplemented by molecular dynamics and continuum micromechanics simulations to predict failure of coral structures under increasing porosity and dissolution. Results reveal remarkable mechanical properties of the building material of cold-water coral skeletons of 462 MPa compressive strength and 45–67 GPa stiffness. This is 10 times stronger than concrete, twice as strong as ultrahigh performance fibre reinforced concrete, or nacre. Contrary to what would be expected, CWCs retain the strength of their skeletal building material despite a loss of its stiffness even when synthesised under future oceanic conditions. As this is on the material length-scale, it is independent of increasing porosity from exposure to corrosive water or bioerosion. Our models then illustrate how small increases in porosity lead to significantly increased risk of crumbling coral habitat. This new understanding, combined with projections of how seawater chemistry will change over the coming decades, will help support future conservation and management efforts of these vulnerable marine ecosystems by identifying which ecosystems are at risk and when they will be at risk, allowing assessment of the impact upon associated biodiversity.
A genome-wide scan of wastewater E. coli for genes under positive selection: focusing on mechanisms of antibiotic resistance
Authors: Malekian Negin , Agrawal Amay A. , Berendonk Thomas U. , Al-Fatlawi Ali , Schroeder Michael 
Published: May 2022

AbstractAntibiotic resistance is a global health threat and consequently, there is a need to understand the mechanisms driving its emergence. Here, we hypothesize that genes and mutations under positive selection may contribute to antibiotic resistance. We explored wastewater E. coli, whose genomes are highly diverse. We subjected 92 genomes to a statistical analysis for positively selected genes. We obtained 75 genes under positive selection and explored their potential for antibiotic resistance. We found that eight genes have functions relating to antibiotic resistance, such as biofilm formation, membrane permeability, and bacterial persistence. Finally, we correlated the presence/absence of non-synonymous mutations in positively selected sites of the genes with a function in resistance against 20 most prescribed antibiotics. We identified mutations associated with antibiotic resistance in two genes: the porin ompC and the bacterial persistence gene hipA. These mutations are located at the surface of the proteins and may hence have a direct effect on structure and function. For hipA, we hypothesize that the mutations influence its interaction with hipB and that they enhance the capacity for dormancy as a strategy to evade antibiotics. Overall, genomic data and positive selection analyses uncover novel insights into mechanisms driving antibiotic resistance.
Identification of QTLs for root color and carotenoid contents in Japanese orange carrot F2 populations
Authors: Shibaya Taeko , Kuroda Chika , Tsuruoka Hisano , Minami Chiharu , Obara Akiko , Nakayama Shinobu , Kishida Yoshie , Fujii Takayoshi , Isobe Sachiko 
Published: May 2022

AbstractCarrot is a major source of provitamin A in a human diet. Two of the most important traits for carrot breeding are carotenoid contents and root color. To examine genomic regions related to these traits and develop DNA markers for carrot breeding, we performed an association analysis based on a general liner model using genome-wide single nucleotide polymorphism (SNPs) in two F2 populations, both derived from crosses of orange root carrots bred in Japan. The analysis revealed 21 significant quantitative trait loci (QTLs). To validate the detection of the QTLs, we also performed a QTL analysis based on a composite interval mapping of these populations and detected 32 QTLs. Eleven of the QTLs were detected by both the association and QTL analyses. The physical position of some QTLs suggested two possible candidate genes, an Orange (Or) gene for visual color evaluation, and the α- and β-carotene contents and a chromoplast-specific lycopene β-cyclase (CYC-B) gene for the β/α carotene ratio. A KASP marker developed on the Or distinguished a quantitative color difference in a different, related breeding line. The detected QTLs and the DNA marker will contribute to carrot breeding and the understanding of carotenoid biosynthesis and accumulation in orange carrots.
Accuracy of a surface-based fusion method when integrating digital models and the cone beam computed tomography scans with metal artifacts
Authors: Zou Bingshuang , Kim Jung-Hoon , Kim So-Hyun , Choi Tae-Hyun , Shin Yonsoo , Kook Yoon-Ah , Lee Nam-Ki 
Published: May 2022

AbstractThe aim of this study was to evaluate the intra- and inter-observer reliability of maxillary digital dental model integration into cone-beam computed tomography (CBCT) scans to reconstruct three-dimensional (3D) skeletodental models for orthognathic patients. This retrospective study consisted of CBCT and digital maxillary dentition images of 20 Class III orthognathic patients. After two repeated fusions of digital cast images with reconstructed CBCT images by a digital engineer and an orthodontist respectively, the 3D coordinate values of the canines, first molars, and central incisors were evaluated. The intra- and inter-observer reliability of 3D positions of maxillary teeth were compared using intraclass correlation coefficients (ICCs). Intra-observer reliability of x-, y-, and z-coordinate values of maxillary teeth showed significant and excellent agreement in an engineer (0.946 ≤ ICC ≤ 1.000) and an orthodontist (0.876 ≤ ICC ≤ 1.000). The inter-observer reliability of the y- and z-coordinates of each tooth was significantly excellent or good, but that of the x-coordinates showed insignificantly poor to moderate agreement. This study showed that the integration of maxillary digital models into CBCT scans was clinically reliable. However, considering the low inter-observer reliability on the x-coordinates of dentition, clinical experience and repeated learning are needed for accurate application of digital skeletodental model in orthognathic patients.
Gain roll-off in cadmium selenide colloidal quantum wells under intense optical excitation
Authors: Diroll Benjamin T. , Brumberg Alexandra , Schaller Richard D. 
Published: May 2022

AbstractColloidal quantum wells, or nanoplatelets, show among the lowest thresholds for amplified spontaneous emission and lasing among solution-cast materials and among the highest modal gains of any known materials. Using solution measurements of colloidal quantum wells, this work shows that under photoexcitation, optical gain increases with pump fluence before rolling off due to broad photoinduced absorption at energies lower than the band gap. Despite the common occurrence of gain induced by an electron–hole plasma found in bulk materials and epitaxial quantum wells, under no measurement conditions was the excitonic absorption of the colloidal quantum wells extinguished and gain arising from a plasma observed. Instead, like gain, excitonic absorption reaches a minimum intensity near a photoinduced carrier sheet density of 2 × 1013 cm−2 above which the absorption peak begins to recover. To understand the origins of these saturation and reversal effects, measurements were performed with different excitation energies, which deposit differing amounts of excess energy above the band gap. Across many samples, it was consistently observed that less energetic excitation results in stronger excitonic bleaching and gain for a given carrier density. Transient and static optical measurements at elevated temperatures, as well as transient X-ray diffraction of the samples, suggest that the origin of gain saturation and reversal is a heating and disordering of the colloidal quantum wells which produces sub-gap photoinduced absorption.
Analysis of circRNAs and circRNA-associated competing endogenous RNA networks in β-thalassemia
Authors: Yang Fang , Ruan Heyun , Li Shuquan , Hou Wei , Qiu Yuling , Deng Lingjie , Su Sha , Chen Ping , Pang Lihong , Lai Ketong 
Published: May 2022

AbstractThe involvement of circRNAs in β-thalassemia and their actions on fetal hemoglobin (HbF) is unclear. Here, the circRNAs in β-thalassemia carriers with high HbF levels were comprehensively analyzed and compared with those of healthy individuals. Differential expression of 2183 circRNAs was observed and their correlations with hematological parameters were investigated. Down-regulated hsa-circRNA-100466 had a strong negative correlation with HbF and HbA2. Bioinformatics was employed to construct a hsa-circRNA-100466‑associated competing endogenous RNA (ceRNA) network to identify hub genes and associated miRNAs. The hsa-circRNA-100466▁miR-19b-3p▁SOX6 pathway was identified using both present and previously published data. The ceRNA network was verified by qRT-PCR analysis of β-thalassemia samples, RNA immunoprecipitation of K562 cell lysates, and dual-luciferase reporter analysis. qRT-PCR confirmed that hsa-circRNA-100466 and SOX6 were significantly down-regulated, while miR-19b-3p was up-regulated. Hsa-circRNA-100466, miR-19b-3p, and SOX6 were co-immunoprecipitated by anti-argonaute antibodies, indicating involvement with HbF induction. A further dual-luciferase reporter assay verified that miR-19b-3p interacted directly with hsa-circRNA-100466 and SOX6. Furthermore, spearman correlation coefficients revealed their significant correlations with HbF. In conclusion, a novel hsa-circRNA-100466▁miR-19b-3p▁SOX6 pathway was identified, providing insight into HbF induction and suggesting targets β-thalassemia treatment.
Worsening drought of Nile basin under shift in atmospheric circulation, stronger ENSO and Indian Ocean dipole
Authors: Mahmoud Shereif H. , Gan Thian Yew , Allan Richard P. , Li Jianfeng , Funk Chris 
Published: May 2022

AbstractUntil now, driving mechanisms behind recurring droughts and hydroclimate variations that controls the Nile River Basin (NRB) remains poorly understood. Our results show significant hydroclimatic changes that contributed to recent increasing aridity of NRB since the 1970s. Besides climate warming, the influence of stronger ENSO and Indian Ocean dipole (IOD) in NRB has increased after 1980s, which have significantly contributed to NRB’s drought severity at inter-annual to inter-decadal timescales. Our results demonstrate that warming, El Niño and IOD have played a crucial role on NRB’s inter-decadal hydroclimate variability, but IOD has played a more important role in modulating NRB’s hydroclimate at higher timescales than El Niño. Results also indicate that the impacts of positive phases of ENSO and IOD events are larger than the negative phases in the NRB hydroclimate. Further, the southward (westward) shift in stream functions and meridional (zonal) winds caused an enhancement in the blocking pattern, with strong anticyclonic waves of dry air that keeps moving into NRB, has resulted in drier NRB, given stream function, geopotential height and U-wind anomalies associated with El Niño shows that changes in regional atmospheric circulations during more persistent and stronger El Niño has resulted in drier NRB. After 1970s, El Niño, IOD, and drought indices shows significant anti-phase relationships, which again demonstrates that more frequent and severe El Niño and IOD in recent years has led to more severe droughts in NRB. Our results also demonstrate that IOD and and the western pole of the Indian Ocean Dipole (WIO) are better predictors of the Nile flow than El Niño, where its flow has decreased by 13.7 (upstream) and by 114.1 m3/s/decade (downstream) after 1964. In summary, under the combined impact of warming and stronger IOD and El Niño, future droughts of the NRB will worsen.
Suitability of spider mites and green peach aphids as prey for Eriopis connexa (Germar) (Coleoptera: Coccinellidae)
Authors: de Matos Sidnéia Terezinha Soares , Savi Patrice Jacob , Melville Cirano Cruz , dos Santos Cividanes Terezinha Monteiro , Cividanes Francisco Jorge , de Andrade Daniel Júnior 
Published: May 2022

AbstractThe ladybird Eriopis connexa is an important natural enemy of several pest arthropods in agroecosystems. High population of this predator is frequently observed in strawberry and soybean crops associated with spider mites. We used two-sex life table parameters to evaluate under laboratory conditions, the suitability of three species of spider mites (Tetranychus evansi, Tetranychus urticae, Tetranychus ogmophallos), and a species of aphid (Myzus persicae) as a prey for the predator E. connexa. Eriopis connexa completed immature development on all prey species except on T. evansi, in which all individuals of predator died before reaching the pupal stage. Among prey species that allowed the immature development of E. connexa, T. urticae and M. persicae provided a faster development time to the predator. Oviposition days, longevity and fecundity of E. connexa on T. urticae and M. persicae were substantially longer/higher than on T. ogmophallos. Net reproductive rate (R0), intrinsic rate of increase (r), and finite rate of increase (λ) of E. connexa feeding on T. urticae and M. persicae were also higher than those on T. ogmophallos. Based on the overall performance of the ladybird, the order of suitability of prey species was M. persicae > T. urticae > T. ogmophallos > T. evansi.
A genome-wide association study for melatonin secretion
Authors: Liu Pi-Hua , Chuang Gwo-Tsann , Hsiung Chia-Ni , Yang Wei-Shun , Ku Hsiao-Chia , Lin Yi-Ching , Chen Yi-Shun , Huang Yu-Yao , Lin Chia-Hung , Li Wen-Yi , Lin Jou-Wei , Hsu Chih-Neng , Hwang Juey-Jen , Liao Karen Chia-Wen , Hsieh Meng-Lun , Lee Hsiao-Lin , Shen Chen-Yang , Chang Yi-Cheng 
Published: May 2022

AbstractMelatonin exerts a wide range of effects among various tissues and organs. However, there is currently no study to investigate the genetic determinants of melatonin secretion. Here, we conducted a genome-wide association study (GWAS) for melatonin secretion using morning urine 6-hydroxymelatonin sulfate-to-creatinine ratio (UMCR). We initially enrolled 5000 participants from Taiwan Biobank in this study. After excluding individuals that did not have their urine collected in the morning, those who had history of neurological or psychiatric disorder, and those who failed to pass quality control, association of single nucleotide polymorphisms with log-transformed UMCR adjusted for age, sex and principal components of ancestry were analyzed. A second model additionally adjusted for estimated glomerular filtration rate (eGFR). A total of 2373 participants underwent the genome-wide analysis. Five candidate loci associated with log UMCR (Pvalue ranging from 6.83 × 10−7to 3.44 × 10−6) encompassingZFHX3,GALNT15,GALNT13,LDLRAD3and intergenic betweenSEPP1andFLJ32255were identified. Similar results were yielded with further adjustment for eGFR. Interestingly, the identified genes are associated with circadian behavior, neuronal differentiation, motor disorders, anxiety, and neurodegenerative diseases. We conducted the first GWAS for melatonin secretion and identified five candidate genetic loci associated with melatonin level. Replication and functional studies are needed in the future.