bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between in vitro fertilization-embryo transfer and hearing loss: risk factors of hearing loss among twin infants in a cohort study
Authors: Yin Jinghua , Su Yu , Siyuan Linpeng , Yin Fei , Wang Weidong , Deng Fengliang , Wang Taosha 
Published: July 2022

Abstract
Purpose:Assisted reproductive technologies (ART), including in vitro fertilization-embryo transfer (IVF-ET), intracytoplasmic sperm injection (ICSI), are recognized contributing higher risk of birth defect, whereas rare studies evaluate the association of IVF-ET with diagnostic hearing loss (HL). This study aimed to evaluate the prevalence and risk factors of HL to clarify the association between IVF-ET and HL among twinborn infants.Methods:We enrolled 1860 live-born twin neonates born in hospital of China from January, 2017 to December, 2020. After examined by two-step hearing screening, participants were finally diagnosed with HL by pediatric audiologists at age of 6 months. Prevalence of hearing loss and odds ratio (OR) for specific risk factors was estimated with generalized estimation equations (GEE) model in twin-born infants. Characteristics and prevalence of failure for hearing screening and HL was measured in IVF-ET twin infants.Results:IVF-ET conception and preterm birth conferred higher risk for hearing loss, with increased adjusted odd ratios (AOR [95% confidence intervals (CI)] IVF-ET: 2.82 [1.17-6.80], P = 0.021; preterm birth: 6.14 [2.30-16.40], P < 0.001). Among 1860 twin infants, more IVF-ET twins failed in dual-step hearing screening (3.26%) and diagnosed with hearing loss (2.21%) compared with spontaneous pregnancy.Conclusions:IVF-ET and premature birth conception were associated with higher risk of hearing impairment. Twin infants conceived by IVF-ET tend to fail in hearing screening and diagnosed with hearing loss. These observations provide a more comprehensive approach for prevention and management of deafness in twin-born children.
Construction and functional enrichment analysis of CeRNA regulatory network for NAION based on high-throughput sequencing
Authors: Zhang meng , Wang xinling 
Published: July 2022

Abstract
Background: Based on the transcriptome high-throughput sequencing of non arteritis anterior ischemic optic neuropathy, this study constructed a competitive endogenous RNA (CeRNA) network , enriched and analyzed it, screened lncRNA and circRNA that may participate in the competitive endogenous mechanism in NAION, and inferred its function. Methods: 4ml peripheral blood of NAION patients and control group were extracted from clinical samples, and the transcriptome high-throughput sequencing was performed, and the sequencing data were visualized; Based on the principle of CeRNA network, lncRNA-miRNA-mRNA interaction axis and circrna-miRNA-mRNA interaction axis were constructed respectively; The differentially expressed genes in the interaction axis were enriched and analyzed by GO and KEGG, and the functions and signal pathways of lncRNA and circRNA in the interaction network were speculated. Results: 51 circRNAs were differentially expressed in the sequencing data: 25 were up-regulated and 26 were down-regulated; There were 996 differentially expressed lncRNAs: 317 were up-regulated and 679 were down-regulated; 1161 differentially expressed mRNAs: 698 were up-regulated and 463 were down-regulated. 33 differentially expressed miRNAs, up-regulated mirna18 and down-regulated 15. After screening, the co-expressed 13 mRNAs, 15 lncRNAs and 3 miRNAs were finally constructed in lncRNA-miRNA-mRNA network and the circRNA-miRNA-mRNA network were constructed by 159 mRNAs, 26 miRNAs and 34 circRNAs. In the lncRNA network , GO enrichment analysis obtained 182 biological processes, 12 cell components and 38 molecular functions, which are mainly related to the activity regulation of proteins and enzymes such as cyclic nucleotide dependent protein kinase activity and magnesium ion dependent protein serine / threonine phosphatase activity. KEGG analysis mainly involves FOXO signal pathway, Apelin signal pathway, etc. In the result of circRNA enrichment, 353 biological processes, 52 cell components and 45 molecular functions were obtained, mainly involving calcium channel regulation, neutrophil activation, mRNA transport and metabolism. KEGG mainly involves Wnt signaling pathway, Apelin signaling pathway, Hippo signaling pathway, oxytocin signaling pathway, etc. Conclusion: This paper provides a new idea for lncRNA and circRNA to mediate the occurrence and development of NAION through CeRNA mechanism. It lays a foundation for the in-depth study of pathogenesis of NAION.
Comparison of three fundus inspection methods during phacoemulsification in patients with diabetic white cataract
Authors: Su Shu , Wu Jian , Ji Min , Guan Yu , Shen Yao , Guan Huaijin 
Published: July 2022

Abstract
Background: The aim of this study was to investigate whether Wild Field Imaging System (WFIS SW-8000), 25G endoilluminator, and intraoperative optical coherence tomography (iOCT) can perform real-time screening and diagnosing in patients with suspicious diabetic retinopathy during phacoemulsification, especially in cases of white cataract that obscures the preoperative view of the retina.Methods: A cross-sectional study was carried out. A total of 204 dense diabetic cataractous eyes of 204 patients with suspected diabetic retinopathy treated at the Affiliated Hospital of Nantong University from April 2020 to March 2021 were included. Lens phacoemulsification combined with intraocular lens implantation was performed. Following the removal of the lens opacity, the 25G endoilluminator, fundus photography, and iOCT were performed successively. Optical coherence tomography (OCT) and/or fundus fluorescein angiography (FFA) were used to verify the fundus findings postoperatively. Intraoperative and postoperative results were compared to verify the accuracy of intraoperative diagnosis in each group.Results: Intraoperative and postoperative examinations revealed 58 and 62 eyes with diabetic retinopathy, respectively (positive rate, 28.43% and 30.39%, respectively). During the phacoemulsification, WFIS SW-8000 detected 44 eyes with diabetic retinopathy (the detection rate, 70.97%); 25G endoilluminator found 56 eyes with diabetic retinopathy (the detection rate, 90.32%); iOCT found 46 eyes with diabetic retinopathy (the detection rate, 74.19%); and 58 eyes with diabetic retinopathy were found by combining the three methods (the detection rate, 93.55%). There were statistically significant differences in the diagnostic sensitivity for diabetic retinopathy among the methods (χ2=16.36, P=0.001).Conclusions: WFIS SW-8000, 25G endoilluminator, iOCT, and especially their combination can be used to inspect the fundus and detect diabetic retinopathy intraoperatively; they are helpful for the timely diagnosis and treatment of retinopathy in patients with dense cataract.
Maternal HDL-c levels are associated with preterm birth and small for gestational age: A prospective study in China
Authors: Chen Cong , Feng Qiuyu , Yang Mengtong , Chen Sijia , Sun Hong , Zhang Yiqi , Pu Shihan , Chen Hong , Su Danping , Guo Yishan , Zeng Guo 
Published: July 2022

Abstract
Background: Evidence suggests that maternal HDL-c levels are associated with preterm birth (PTB) and small for gestational age (SGA). However, existing evidence remains controversial. We aimed to explore the associations of maternal HDL-c levels with PTB and SGA in China.Method: A prospective study that included 1068 pregnant women was conducted in 2017. The concentrations of high-density lipoprotein cholesterol (HDL-c) were analyzed in early, middle, and late pregnancy as continuous variables and categorical variables (by percentile groups). Data on gestational age, as well as the weight and length of the neonates were recorded after delivery. Multivariable logistic regression models were used to estimate the associations between maternal HDL-c levels and PTB and SGA. Result:PTB and SGA occurred in 3.0% and 8.1% of births. Mean maternal HDL-c levels in early pregnancy was equal to that in middle pregnancy (1.67 vs. 1.66 mmol/L, p = 0.309), but decreased slightly from middle pregnancy to late pregnancy (1.66 vs. 1.52mmol/L, p < 0.001). In middle pregnancy, every unit elevation of HDL-c was positively associated with SGA (OR = 2.57, 95 % CI: 1.23-5.35). In late pregnancy, every unit elevation of HDL-c reduced the risk of PTB (OR = 0.12, 95 % CI: 0.03-0.58), but increased the risk of SGA (OR = 2.59, 95 % CI: 1.22-5.47). In early pregnancy, low (<25th percentile) HDL-c was positively associated with PTB (OR = 2.56, 95 % CI: 1.11-5.09), and high (>75th percentile) HDL-c was positively associated with SGA (OR = 2.92, 95 % CI: 1.69-5.04), compared with referent (25th-75th percentile) HDL-c.Conclusion:Maternal high levels of HDL-c could reduce the risk of PTB but increase the risk of SGA. There may exist appropriate reference range for maternal HDL-c in early pregnancy. Maternal HDL-c levels should be monitored regularly during pregnancy to decrease adverse perinatal outcomes.
Rapid and precise identification of bloodstream infections using a pre-treatment protocol combined with high-throughput multiplex genetic detection system
Authors: Zhang Jinghao , Yang Feng , Sun Zhaoyang , Fang Yi , Zhu Haowei , Zhang Dijun , Zeng Xianping , Liu Wenjian , Liu Tao , Liu Yixin , Chi Wenjing , Wang Su , Ding Li , Wu Yong , Zhang Yanmei , Zhao Hu 
Published: July 2022

Abstract
Background
Bloodstream infection (BSI) is a life-threatening condition with high rates of morbidity and mortality worldwide. Early diagnosis of BSI is critical to avoid unnecessary application of antimicrobial agents and for proper treatment. However, the current standard methods based on blood culture are time-consuming, thus failing to provide timely etiological diagnosis of BSI, and common PCR-based detection might be inhibited by matrix components.
Methods
The current study explored an integrated pre-analytical treatment protocol for whole blood samples, wherein pathogens are enriched and purified by incubation and concentration, and inhibitors are inactivated and removed. Further, this study developed and evaluated a novel high-throughput multiplex genetic detection system (HMGS) to detect 24 of the most clinically prevalent BSI pathogens in blood culture samples as well as pre-treated whole blood samples. The specificity and sensitivity were evaluated using related reference strains and quantified bacterial/fungal suspensions. The clinical utility of BSI-HMGS combined with the pre-analytical treatment protocol was verified using blood cultures and whole blood samples.
Results
The combined pre-treatment protocol and BSI-HMGS was highly specific for target pathogens and possessed a low detection limit for clinical whole blood samples. The pre-treatment protocol could deplete the PCR inhibitors effectively. For blood culture samples, the current method showed 100.0% negative percent agreements and > 87.5% positive percent agreements compared to the reference results based on blood culture findings. For whole blood samples, the current method showed 100.0% negative percent agreements and > 80.0% positive percent agreements compared to the reference results for most pathogens. The turnaround time was ≤ 8 h and all the procedures could be conducted in a general clinical laboratory.
Conclusion
The BSI-HMGS combined with the pre-treatment protocol was a practical and promising method for early and precise detection of BSIs, especially for the areas without access to advanced medical facilities.
The Dyslexia-associated gene KIAA0319L is involved in neuronal migration in the developing chick visual system
Authors: Charish Jason , Harada Hidekiyo , Chen Xiaoyan , Wälchli Thomas , Barr Cathy L. , Monnier Philippe 
Published: July 2022

Abstract
The gene KIAA0319-Like ( KIAA0319L ) is thought to confer susceptibility for developmental dyslexia. Dyslexia can be caused by defects in neuronal migration, however, studies done in KIAA0319L knock-out mice did not reveal any neuronal migration phenotype. Gene knock out may activate compensatory mechanisms to buffer against genetic mutations. Here we re-assessed the role of KIAA0319L on migrating neurons in the chick developing tectum. Whole mount in situ hybridization was performed for KIAA0319L on embryonic day (E)3 – E5 chick embryos and in situ hybridization on sections was performed at later stages. Engineered microRNA (miRNA) constructs targeting KIAA0319L were prepared and their specificity and efficiency for knocking down KIAA0319L were tested. miRNAs were electroporated in E5 chick optic tecta (OT). Our studies demonstrate that KIAA0319L is expressed in the developing chick visual system as well as the otic vescicles. Knockdown of KIAA0319L in the OT results in abnormal neuronal migration, strengthening the argument that KIAA0319L is involved in this developmental process.
High expression of IFITM2 in gastric cancer is related to poor prognosis
Authors: Liu Yonggang , Li Xi , Huang Junyong , Huang Jiangyuan , Zhu Qiaohua , Wang Jiale , Luo Meihua 
Published: July 2022

Abstract
Introduction IFITM2 (interferon-induced transmembrane protein 2) is involved in the repression of viral infection. Recently, IFITM2 was found to be highly expressed in multiple cancers. However, the possible roles of IFITM2 in gastric cancer (GC) remain unclarified. In this research, we determined the expression of IFITM2 and its relationship with clinical outcome in GC.Methods The original data retrieved from bioinformatics databases were integrated by R package v3.6.2. The expression of IFITM2 in GC was explored by online databases of UALCAN. Immunohistochemistry staining and molecular analysis were conducted to validate IFITM2 expression in human GC cells and clinical specimens. The association between IFITM2 and clinicopathological features were analyzed by univariate/multivariate Cox regression. The prognostic significance of IFITM2 gene expression in the TCGA-STAD and GEO database were evaluated by TIMER and Kaplan-Meier Plotter tool. The relationships between IFITM2 and immune tumor characteristics were analyzed with TIMER 2.0. The biological function of IFITM2 were analyzed by GSEA. IFITM2-specific siRNA was employed to confirm the roles of IFITM2 in cell migration and wound healing.Results IFITM2 was over-expressed in GC and contributed to poor prognosis. High IFITM2 expression in GC was obviously related to T stage, distant metastasis, higher histologic grade. IFITM2 was a significant risk factor influencing the survival of GC patients. High IFITM2 expression was positively associated related to immune-infiltrating cells. The Cytokine-Cytokine-Receptor-Interaction pathway was remarkably enriched by IFITM2 upregulation. IFITM2 knockdown was found to significantly reduce the ability of cell migration and scratch repair.Conclusion IFITM2 is over-expressed in GC and affects the prognosis of GC patients. IFITM2 may act as a novel prognostic biomarker for GC patients.
Attempts to quit smoking, use of smoking cessation methods, and associations with person characteristics among patients with chronic obstructive pulmonary disease
Authors: Kastaun Sabrina , Pashutina Yekaterina , Kotz Daniel 
Published: July 2022

Abstract
IntroductionWe explored past-year quit attempts, cessation methods used, and associations with person characteristics among smoking patients with chronic obstructive pulmonary disease (COPD) in Germany.MethodsCross-sectional survey data of 509 past-year smokers (current smokers and ≤12 months abstinent) with spirometry-confirmed COPD from 19 pulmonary primary care practices were used. Associations were explored between sociodemographic characteristics, lung function, urges to smoke, psychological distress, and ≥1 past-year quit attempt (yes/no)/use of ≥1 evidence-based cessation method (yes/no). ResultsOf all patients, 48.5% (n=247) reported ≥1 past-year quit attempt. Male gender was positively and time spent with urges to smoke was negatively associated with such attempt. One third used ≥1 evidence-based cessation method. Strength of urges to smoke was positively associated with such use. Combined behavioural-pharmacological therapy was used by 4.0%. Electronic cigarettes were used most frequently (21.5%). ConclusionsQuit attempt prevalence among smoking COPD patients in Germany pulmonary primary care is high, but only a small proportion of them support their attempt with treatments as recommended in clinical guidelines.
Diagnostic value of spiral CT energy spectrum imaging in pathological differentiation of colorectal cancer
Authors: Chen weibin , Li zemao , Kang liqing 
Published: July 2022

Abstract
Objective: To investigate the accuracy of CT energy spectrum imaging in predicting preoperative pathological differentiation of colorectal cancer patients.Methods: From 2019 September to 2021 November, a retrospective study was performed for the eighty-two patients with colorectal cancer through preoperative colonoscopy or surgical pathology confirmed in our hospital. According to the pathological results, the patients were divided into middle and high differentiation group (62 cases) and low differentiation group (20 cases). GE Revolution CT scanner was used to scan the patients with energy spectrum imaging, in the arterial and venous phase, it measured and recorded the single energy CT values from 40kev to 140kev and various energy spectrum parameters of lymph nodes around the lesions. And statistically analyze the above indices.Result: in arterial phase: the single energy CT values from 40kev to 140kev in the low differentiation group were higher than those in the medium and high differentiation group (all P <0.05); IC, NIC and λ of colorectal cancer in low differentiation group were higher than that in medium and high differentiation group(all P<0.05). The ROC curve showed that the AUC of the single energy CT values from 40kev to 140kev were less than 0.800. The AUC of IC and NIC were 0.800 and 0.828 respectively; the highest AUC of the combination of IC and NIC was 0.850. in venous phase: the single energy CT values from 40kev to 130kev in the low differentiation group were higher than those in the medium and high differentiation group(all P<0.05), and IC, NIC, λ and Eff-Z of colorectal cancer in low differentiation group were higher than that in the medium and high differentiation group(all<0.05). The ROC curve showed that the AUC of the single energy CT values from 40kev to 90kev were higher than 0.800. The AUC of IC, NIC, λ and Eff-Z were 0.927, 0.882, 0.859 and 0.863 respectively; the parameters were combined in pairs, IC and λ achieved the highest AUC, and the highest AUC was 0.934. Three parameters were combined, IC, NIC and λ achieved the highest AUC, and the highest AUC was 0.942, the combined AUC of the four was 0.943. Compared with the arterial phase, the single energy CT values from 40kev to 90kev and energy spectrum parameters in venous phase had better diagnostic efficacy in predicting different degrees of differentiation of colorectal cancer.Conclusion: energy spectrum CT imaging can better predict the differentiation grade of colorectal cancer before operation and provide imaging basis for clinicians.
Participation Intention and Needs of Older people towards First Aid Training--A Qualitative Study
Authors: Yin Guo , Lin Siting , Chen Linghui 
Published: July 2022

Abstract
Background
Older people are at high risk of accidental injuries, sudden diseases and other adverse events. First aid skills for older people can enable them to rescue themselves in times of crisis and avoid disability and death. This study aimed to explore Chinese older people's participation intentions and needs towards first aid skills training.
Methods
This study used phenomenological methods in qualitative research. A total of 28 older people were interviewed from November 2021 to February 2022. The data were analyzed by using Colaizzi content analysis methods.
Results
Three themes were extracted from the interview data: First aid training participation intention; First aid training participation barriers; First aid training recommendations.
Conclusion
Older people have a strong intention to participate in first aid training. First aid training is feasible for older people, but the first aid training content and plans should be formulated according to their physical and mental characteristics, as well as their needs.
Phenotypic Characterization of Bipolaris oryzae in Fogera Plain, Ethiopia
Authors: Ejigu Muluadam Berhan , Admasu Merkuz Abera , Feyissa Birhanu Bekele 
Published: July 2022

Abstract
Rice (Oryzae sativa L.) is one of the most important cereal crops on which an ever-increasing population is dependent for food globally. However, constraints associated with infectious diseases, notably brown spot transmitted by seed and wind reduced its production and productivity. This study was, therefore, conducted with the aim of morphological characterization of Bipolaris oryzae, the causal pathogen of brown spot isolates and screening its resistant Lowland rice pipelines under natural infestation rain-fed conditions in Fogera plains of Amhara region, Ethiopia. Twenty-nine Bipolaris oryzae isolates were collected from Dera, Fogera, and Libokemkem districts and characterized morphologically at the Ambo Agricultural Research Center plant pathology laboratory. These isolates were grouped into nine categories on their colony morphology and growth pattern. Fast, moderate and slow mycelial growth habits were also observed in 29 isolates with varied conidia size and sporulation rates. All tested genotypes and isolates were clustered into four groups and strong correlations were observed between various parameters. However, it is advised to identify inherent resistance genes of the above lines in combination with the pathogen genetic characteristics for fruitful management recommendations.
Star-shaped incision technique for gingiva patients treated with implant-supported fixed prosthesis
Authors: Luo Wen , Kuang Huifang , Wang Jinrong , Zheng Kaiyue , Li Zhixin , Qu Yili , Man Yi , Wu Yingying 
Published: July 2022

Abstract
Background: To evaluate the modified star-shaped incision on gingival sulcus for reducing horizontal food impaction around implant-supported restoration.Methods: Total 24 patients receiving bone-level implant placement were enrolled, a star-shaped incision was made on the gingiva sulcus before the placement of zirconia crown. Follow-up examination was carried out 3 and 6 months after final restoration, respectively. Assessment of soft tissue includes papilla height, modified plaque index, modified sulcus bleeding index, periodontal depth, gingival biotype and gingival margin level. Marginal bone level was measured on periapical radiographs. Results: Only 1 patient complained about the horizontal food impaction. Both the mesial and distal papilla almost filled the entire proximal space, in good harmony with the adjacent papillae. No recession of the gingival margin was found around the crown even in the patients with thin gingival biotype. Other parameters of soft tissue including modified plaque index, modified sulcus bleeding index and periodontal depth remained low during the whole follow-up visit. The resorption of marginal crestal bone was less than 0.6 mm during the first 6 month, and there was no significant difference among baseline, 3-month and 6-month visit. Conclusion: The modified star-shaped incision on the gingiva sulcus maintained the gingival papilla height and reduced the occurrence of horizontal food impaction, and no recession of the gingiva margin was found around implant-supported restoration.