bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of native cyclopoid copepods in biological vector control with regard to their predatory behavior against the Asian tiger mosquito, Aedes albopictus
Authors: Pauly Isabel , Jakoby Oliver , Becker Norbert 
Published: October 2022

Abstract
Background
The control of the Asian tiger mosquito Aedes albopictus (Diptera: Culicidae) is crucial owing to its high vector competence for more than 20 arboviruses—the most important being dengue, chikungunya and Zika virus. Aedes albopictus has an enormous adaptive potential, and its invasive spreading across urban and suburban environments poses challenges for its control. Therefore, all suitable, cost-effective and eco-friendly control tools should be put into practice. In this context, cyclopoid copepods are already known as effective predators of mosquito larvae. This study reports an essential preliminary step towards the integration of copepods into the vector control strategy in Germany, in order to provide a sustainable tool in an integrated control strategy based on the elimination or sanitation of breeding sites, the use of formulations based on Bacillus thuringiensis israelensis (Bti.) and the sterile insect technique (SIT).

Methods
The predatory potential of native cyclopoid copepods, namely the field-derived species Megacyclops viridis (Crustacea: Cyclopidae), was examined against the larvae of Ae. albopictus, and for comparison, against the larvae of the common house mosquito, Culex pipiens sensu lato (Diptera: Culicidae). The use of different larval instars as prey, and various predator-to-prey ratios, were examined under laboratory and semi-field conditions. The compatibility of Bti. applications along with the use of copepods was assessed in the laboratory.

Results
High predation efficiency of M. viridis upon first-instar larvae of Ae. albopictus was observed under laboratory (up to 96%) and semi-field conditions (65.7%). The copepods did not prey upon stages further developed than the first instars, and in comparison with Ae. albopictus, the predation rates on the larvae of Cx. pipiens s.l. were significantly lower.

Conclusions
The results indicate a high predation potential of M. viridis against Ae. albopictus larvae, even though strong larval stage and mosquito species preferences were implicated. The integration of copepods as a promising biocontrol agent to the vector control strategy in Germany is therefore highly recommended, especially because of the excellent compatibility of copepods with the use of Bti. However, further research is required, concerning all the probable parameters that may impact the copepod performance under natural conditions.

Graphical Abstract
Identification of differentially expressed genes based on antennae RNA-seq analyses in Culex quinquefasciatus and Culex pipiens molestus
Authors: Gao Heting , Gu Zhenyu , Xing Dan , Yang Qiaojiang , Li Jianhang , Zhou Xinyu , Zhao Teng , Li Chunxiao 
Published: October 2022

AbstractBackgroundBothCulex quinquefasciatusandCx. pipiens molestusare sibling species withinCx. pipienscomplex. Even though they are hard to distinguish morphologically, they have different physiological behaviors. However, the molecular mechanisms underlying these differences remain poorly understood.MethodsTranscriptome sequencing was conducted on antennae of two sibling species. The identification of the differentially expressed genes (DEGs) was performed by the software DESeq2. Database for Annotation, Visualization and Integrated Discovery was used to perform GO pathway enrichment analysis. The protein–protein interaction (PPI) network was constructed with Cytoscape software. The hub genes were screened by the CytoHubba plugin and Degree algorithms. The identified genes were verified by quantitative real-time PCR.ResultsMost annotated transcripts (14,687/16,005) were expressed in both sibling species. Among 15 identified odorant-related DEGs,OBP10was expressed 17.17 fold higher inCx. pipiens molestusthanCx. quinquefasciatus. Eighteen resistance-related DEGs were identified, including 15 fromCYPgene family and three from acetylcholinesterase, in whichCYP4d1was 86.59 fold more highly expressed inC. quinquefasciatus. Three reproductive DEGs were indentified with the expression from 5.01 to 6.55 fold. Among eight vision-related DEGs, retinoic acid receptor RXR-gamma inCx. pipiens molestusgroup was more expressed with 214.08 fold. Among the 30 hub genes, there are 10 olfactory-related DEGs, 16 resistance-related DEGs, and four vision-related DEGs, with the highest score hub genes beingOBP lush(6041148),CYP4C21(6044704), andRdh12(6043932). The RT-qPCR results were consistent with the transcriptomic data with the correlation coefficientR = 0.78.ConclusionThe study provided clues that antennae might play special roles in reproduction, drug resistance, and vision, not only the traditional olfactory function.OBP lush,CYP4C21, andRdh12may be key hints to the potential molecular mechanisms behind the two sibling species' biological differences.Graphical Abstract
Comparison of PSMA-TO-1 and PSMA-617 labeled with gallium-68, lutetium-177 and actinium-225
Authors: Meyer Catherine , Prasad Vikas , Stuparu Andreea , Kletting Peter , Glatting Gerhard , Miksch Jonathan , Solbach Christoph , Lueckerath Katharina , Nyiranshuti Lea , Zhu Shaojun , Czernin Johannes , Beer Ambros J. , Slavik Roger , Calais Jeremie , Dahlbom Magnus 
Published: October 2022

Abstract
Background
PSMA-TO-1 (“Tumor-Optimized-1”) is a novel PSMA ligand with longer circulation time than PSMA-617. We compared the biodistribution in subcutaneous tumor-bearing mice of PSMA-TO-1, PSMA-617 and PSMA-11 when labeled with 68Ga and 177Lu, and the survival after treatment with 225Ac-PSMA-TO-1/-617 in a murine model of disseminated prostate cancer. We also report dosimetry data of 177Lu-PSMA-TO1/-617 in prostate cancer patients.

Methods
First, PET images of 68Ga-PSMA-TO-1/-617/-11 were acquired on consecutive days in three mice bearing subcutaneous C4-2 xenografts. Second, 50 subcutaneous tumor-bearing mice received either 30 MBq of 177Lu-PSMA-617 or 177Lu-PSMA-TO-1 and were sacrificed at 1, 4, 24, 48 and 168 h for ex vivo gamma counting and biodistribution. Third, mice bearing disseminated lesions via intracardiac inoculation were treated with either 40 kBq of 225Ac-PSMA-617, 225Ac-PSMA-TO-1, or remained untreated and followed for survival. Additionally, 3 metastatic castration-resistant prostate cancer patients received 500 MBq of 177Lu-PSMA-TO-1 under compassionate use for dosimetry purposes. Planar images with an additional SPECT/CT acquisition were acquired for dosimetry calculations.

Results
Tumor uptake measured by PET imaging of 68Ga-labeled agents in mice was highest using PSMA-617, followed by PSMA-TO-1 and PSMA-11. 177Lu-PSMA tumor uptake measured by ex vivo gamma counting at subsequent time points tended to be greater for PSMA-TO-1 up to 1 week following treatment (p > 0.13 at all time points). This was, however, accompanied by increased kidney uptake and a 26-fold higher kidney dose of PSMA-TO-1 compared with PSMA-617 in mice. Mice treated with a single-cycle 225Ac-PSMA-TO-1 survived longer than those treated with 225Ac-PSMA-617 and untreated mice, respectively (17.8, 14.5 and 7.7 weeks, respectively; p < 0.0001). Kidney, salivary gland, bone marrow and mean ± SD tumor dose coefficients (Gy/GBq) for 177Lu-PSMA-TO-1 in patients #01/#02/#03 were 2.5/2.4/3.0, 1.0/2.5/2.3, 0.14/0.11/0.10 and 0.42 ± 0.03/4.45 ± 0.07/1.8 ± 0.57, respectively.

Conclusions
PSMA-TO-1 tumor uptake tended to be greater than that of PSMA-617 in both preclinical and clinical settings. Mice treated with 225Ac-PSMA-TO-1 conferred a significant survival benefit compared to 225Ac-PSMA-617 despite the accompanying increased kidney uptake. In humans, PSMA-TO-1 dosimetry estimates suggest increased tumor absorbed doses; however, the kidneys, salivary glands and bone marrow are also exposed to higher radiation doses. Thus, additional preclinical studies are needed before further clinical use.
Safety assessment of compliant, highly invasive, lipid A-altered, O-antigen-defected Salmonella strains as prospective vaccine delivery systems
Authors: Aganja Ram Prasad , Sivasankar Chandran , Hewawaduge Chamith , Lee John Hwa 
Published: October 2022

AbstractIn the present study, two prospective Salmonella delivery strains, JOL2782 and JOL2837, were developed by gene deletions of lon and cpxR, which are related to cellular adhesion and intracellular survival. Additionally, sifA deletion was introduced for JOL2782, which confers immune susceptibility and improves antigen delivery. Similarly, the rfaL deletion and lpxE substitution for pagL were accomplished in JOL2837 to reduce virulence and endotoxicity. Thus, enhanced adhesion and invasion and reduced intracellular survival were attained. Furthermore, aspartic acid auxotrophic (asd) was deleted to impose Darwinian selection on retention of the foreign antigen-expressing plasmid. Both delivery strains induced sufficient cytokine expression, but the level was significantly lower than that of the wild-type strain; the lowest cytokine expression was induced by the JOL2837 strain, indicating reduced endotoxicity. In parallel, IgG production was significantly enhanced by both delivery strains. Thus, the innate and adaptive immunogenicity of the strains was ensured. The environmental safety of these strains was ascertained through faecal dissemination assays. The nonpathogenicity of these strains to the host was confirmed by body weight monitoring, survival assays, and morphological and histological assessments of the vital organs. The in vitro assay in murine and human cell lines and in vivo safety assessments in mice suggest that these novel strains possess safety, invasiveness, and immunogenicity, making them ideal delivery strains. Overall, the results clearly showed that strain JOL2782 with sifA deletion had higher invasiveness, demonstrating superior vaccine deliverability, while JOL2837 with lpxE substitution for pagL and rfaL deletion had outstanding safety potential with drastically abridged endotoxicity.
cAMP signaling factors regulate carbon catabolite repression of hemicellulase genes in Aspergillus nidulans
Authors: Kunitake Emi , Uchida Ryota , Asano Keisuke , Kanamaru Kyoko , Kimura Makoto , Kimura Tetsuya , Kobayashi Tetsuo 
Published: October 2022

AbstractCarbon catabolite repression (CCR) enables preferential utilization of easily metabolizable carbon sources, implying the presence of mechanisms to ensure discriminatory gene repression depending on the ambient carbon sources. However, the mechanisms for such hierarchical repression are not precisely understood. In this report, we examined how deletion of pkaA and ganB, which encode cAMP signaling factors, and creA, which encodes a well-characterized repressor of CCR, affects CCR of hemicellulase genes in the filamentous fungus Aspergillus nidulans. β-Xylanase production increased not only in ΔcreA but also in ΔpkaA and ΔganB, with the highest level observed in their double deletants, irrespective of the presence or absence of d-glucose. Expression of the β-xylanase genes in the presence of d-glucose was de-repressed in all the deletion mutants, with significantly higher tolerance against d-glucose repression in ΔpkaA and ΔganB than in ΔcreA. In the presence of galactomannan and d-glucose, partial de-repression of β-mannanase production was detected in ΔcreA, but not in ΔpkaA and ΔganB. The double deletion of creA/pkaA and creA/ganB led to earlier production. Release from d-glucose repression of the β-mannanase genes was partial in the single deletants, while nearly full de-repression was observed in ΔcreAΔpkaA and ΔcreAΔganB. The contribution of PkaA and GanB to CCR by d-xylose of the β-mannanase genes was very minor compared to that of CreA. Consequently, the present study revealed that cAMP signaling plays a major role in CCR of hemicellulase gene expression in a manner that is clearly independent from CreA.
Revisiting potential value of antitumor drugs in the treatment of COVID-19
Authors: Zheng Wenfang , Zeng Zekun , Lin Shumei , Hou Peng 
Published: October 2022

AbstractSince an outbreak started in China in 2019, coronavirus disease 2019 (COVID-19) has rapidly become a worldwide epidemic with high contagiousness and caused mass mortalities of infected cases around the world. Currently, available treatments for COVID-19, including supportive care, respiratory support and antiviral therapy, have shown limited efficacy. Thus, more effective therapeutic modalities are highly warranted. Drug repurposing, as an efficient strategy to explore a potential broader scope of the application of approved drugs beyond their original indications, accelerates the process of discovering safe and effective agents for a given disease. Since the outbreak of COVID-19 pandemic, drug repurposing strategy has been widely used to discover potential antiviral agents, and some of these drugs have advanced into clinical trials. Antitumor drugs compromise a vast variety of compounds and exhibit extensive mechanism of action, showing promising properties in drug repurposing. In this review, we revisit the potential value of antitumor drugs in the treatment of COVID-19 and systematically discuss their possible underlying mechanisms of the antiviral actions.
YAP1 protects against septic liver injury via ferroptosis resistance
Authors: Wang Jin , Zhu Qian , Li Rui , Zhang Jing , Ye Xujun , Li Xinyi 
Published: October 2022

Abstract
Background
The liver plays crucial roles in sepsis and is one of the major targets for sepsis-related injuries. Ferroptosis, a newly emerged form of lytic cell death, has been implicated in sepsis related organ failure. Yes-associated protein1 (YAP1), a key regulator of the Hippo signaling pathway, may be involved in ferroptosis development. This study aimed to elucidate the role of YAP1 in septic liver injury through regulating ferroptosis, especially ferritinophagy-mediated ferroptosis.

Results
Cecal ligation and puncture (CLP) models were constructed in control (Yap1flfl) and liver-conditional knockout mice (Yap1fl/fl Alb-Cre) to induce septic liver injury, while LO2 cells with or without YAP1 overexpression/deletion were stimulated by lipopolysaccharide (LPS) in vitro. Our study showed YAP1 knockdown aggravated CLP-induced liver injury and inflammation, as well as accelerated hepatocyte ferroptosis, revealed by down-regulated expression of GPX4, FTH1 and SLC7A11, along with up-regulated expression of SFXN1 and NCOA4. Consistently, YAP1 deficiency aggravated LO2 cells ferroptosis, but YAP1 overexpression alleviated LPS-induced LO2 ferritinophagy, as evidenced by reduced mitochondrial ROS and Fe2+, along with down-regulated expression of SFXN1 and NCOA4. Further co-IP assay verified that YAP1 disrupted the interaction between NCOA4 and FTH1, thus prevent the degradation of ferritin to Fe2+, further reduced the ROS production and suppressed ferroptosis.

Conclusion
YAP1 inhibits ferritinophagy-mediated ferroptosis in hepatocytes, and YAP1 deficiency aggravates sepsis-induced liver injury.
Comparison of ultrasound-guided internal jugular vein and supraclavicular subclavian vein catheterization in critically ill patients: a prospective, randomized clinical trial
Authors: Trabelsi Becem , Hajjej Zied , Drira Dhouha , Yedes Azza , Labbene Iheb , Ferjani Mustapha , Ben Ali Mechaal 
Published: October 2022

Abstract
Background
The aim of this study was to compare the effectiveness and safety of ultrasound-guided out-of-plane internal jugular vein (OOP-IJV) and in-plane supraclavicular subclavian vein (IP-SSCV) catheterization in adult intensive care unit.

Methods
A total of 250 consecutive patients requiring central venous catheterization, were randomly assigned to undergo either ultrasound-guided OOP-IJV or IP-SSCV cannulation. All catheterizations were carried out by three physicians. The primary outcome was the first attempt success rate. Ultrasound scanning time, venous puncture time, insertion time, overall access time, number of puncture attempts, number of needle redirections, success rate, guidewire advancing difficulties, venous collapse and adverse events were also documented.

Results
The first attempt success rate was significantly higher in IP-SSCV group (83.2%) compared to OOP-IJV group (63.2%) (p = 0.001). The IP-SSCV group was associated with a longer ultrasound scanning time (16.54 ± 13.51 vs. 5.26 ± 4.05 s; p < 0.001) and a shorter insertion time (43.98 ± 26.77 vs. 53.12 ± 40.21 s; p = 0.038). In the IP-SCCV group, we recorded a fewer number of puncture attempts (1.16 ± 0.39 vs. 1.47 ± 0.71; p < 0.001), needle redirections (0.69 ± 0.58 vs. 1.17 ± 0.95; p < 0.001), difficulties in guidewire advancement (2.4% vs. 27.4%; p < 0.001), venous collapse (2.4%, vs. 18.4%; p < 0.001) and adverse events (8.8% vs. 13.6%; p = 0.22).

Conclusions
The IP-SSCV approach is an effective and a safe alternative to the classic OOP-IJV catheterization in critical adult patients.
Trial registration: Clinicaltrials.gov, NCT03879954. Registered March 19, 2019—Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT03879954.
Multilayer graph spectral analysis for hyperspectral images
Authors: Zhang Songyang , Deng Qinwen , Ding Zhi 
Published: October 2022

AbstractHyperspectral imaging has broad applications and impacts in areas including environmental science, weather, and geo/space exploration. The intrinsic spectral–spatial structures and potential multi-level features in different frequency bands make multilayer graph an intuitive model for hyperspectral images (HSI). To study the underlying characteristics of HSI and to take the advantage of graph signal processing (GSP) tools, this work proposes a multilayer graph spectral analysis for hyperspectral images based on multilayer graph signal processing (M-GSP). More specifically, we present multilayer graph (MLG) models and tensor representations for HSI. By exploring multilayer graph spectral space, we develop MLG-based methods for HSI applications, including unsupervised segmentation and supervised classification. Our experimental results demonstrate the strength of M-GSP in HSI processing and spectral–spatial information extraction.
A designated verifier multi-signature scheme in multi-clouds
Authors: Tan Chaoyue , Chen Yuling , Wu Yongtang , He Xiaochuan , Li Tao 
Published: October 2022

AbstractMulti-cloud computing provides services by used different clouds simultaneously multi-signature can be used as the interactive technology between multi-cloud and users. However, the limited resources of some terminal devices make multi-signature, which based on bilinear map, is not suitable for multi-cloud computing environment. In addition, the signers are disclosure in multi-signature so there is the risk of attack. To solve this issues, this paper proposes a certificateless designated verifier multi-signature scheme based on multivariable public key cryptography (MPKC). Firstly, the formalized definition and security model of the proposed scheme are given. Secondly, it is proved that the proposed scheme is against adaptive chosen-message attacks. Finally, the analysis shows that the proposed scheme is more efficiency and suitable for multi-cloud. Moreover, the proposed scheme can hidding signature source to achieve privacy protection.
Defect knowledge graph construction and application in multi-cloud IoT
Authors: Yang Wenqing , Li Xiaochao , Wang Peng , Hou Jun , Li Qianmu , Zhang Nan 
Published: October 2022

AbstractAs the State Grid Multi-cloud IoT platform grows and improves, an increasing number of IoT applications generate massive amounts of data every day. To meet the demands of intelligent management of State Grid equipment, we proposed a scheme for constructing the defect knowledge graph of power equipment based on multi-cloud. The scheme is based on the State Grid Multi-cloud IoT architecture and adheres to the design specifications of the State Grid SG-EA technical architecture. This scheme employs ontology design based on a fusion algorithm and proposes a knowledge graph reasoning method named GRULR based on logic rules to achieve a consistent and shareable model. The model can be deployed on multiple clouds independently, increasing the system’s flexibility, robustness, and security. The GRULR method is designed with two independent components, Reasoning Evaluator and Rule Miner, that can be deployed in different clouds to adapt to the State Grid Multi-cloud IoT architecture. By sharing high-quality rules across multiple clouds, this method can avoid vendor locking and perform iterative updates. Finally, the experiment demonstrates that the GRULR method performs well in large-scale knowledge graphs and can complete the reasoning task of the defect knowledge graph efficiently.
Trends and focuses of hantavirus researches: a global bibliometric analysis and visualization from 1980 to 2020
Authors: Wei Xiao , Li Xinlou , Song Shuxuan , Wen Xiaohui , Jin Tiezhi , Zhao Chenxi , Wu Xubin , Liu Kun , Shao Zhongjun 
Published: October 2022

Abstract
Background
There have been worldwide changes in the researches on hantaviruses in the past several decades. Nevertheless, there are few bibliometric analysis studies this field. We aim to evaluate and visualize the research focuses and trends of this field using a bibliometric analysis way to help understand the developmet and future hotspots of this field.

Material and methods
Publications related to hantavirus studies were culled from the Web of Science Core Collection to generate trend analysis. The articles and reviews were re-extracted and Countries, institutions, authors, references and keywords in this field were visually analyzed by using VOSviewer and CiteSpace.

Results
A total of 4408 studies were included and the number of publications regarding hantaviruses significantly increased yearly. Three thousand seven hundred sixteen research articles and reviews were retrieved to generate bibliometric analysis. These studies mainly come from 125 countries led by USA and China and 3312 institutions led by the University of Helsinki. Twelve thousand five hundred twenty nine authors were identified and Vaheri A were the most influential author. Journal of Virology was the journal with the most studies and citations. After analysis, Hemorrhagic fever with renal syndrome, Hantavirus cardiopulmonary syndrome, nephropathia epidemica and related genotypes, clinical symptoms and rodents were the most common keywords and developing areas.

Conclusion
Research on hantavirus is flourishing. Cooperation among different countries and institutions in this field must be strengthened in the future. The ecology and clinical symptoms of new genotypes, the vaccine development and factors that affect host population distribution and density are current and developing areas of study.