bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcomes of the transsphenoidal approach for ACTH-secreting pituitary tumours; late recurrences and delayed remission after surgery of Cushing’s disease.
Authors: Saratziotis Athanasios , Munari Sara , Zanotti Claudia , Baldovin Maria , Denaro Luca , Ferfeli Maria , Hajiioannou Jiannis , Emanuelli Enzo 
Published: June 2022

Abstract
Background: To report surgical outcomes of transsphenoidal surgery as first-line treatment and predictors of remission in a specialist centre for patients with Cushing’s disease and to investigate long-term remission and delayed recurrence. Methods: Clinical data was obtained from a retrospective CD database while all electronic medical, laboratory and surgical patient records between 2012 and 2022 with a minimum 23 months’ follow-up, were evaluated. Result: In this single-centre study a total of 50 consecutive patients with CD (34F, 16M) underwent TSS. Median (range) age and follow-up were 37.8 (15-69) and 76.5 (23-122) months respectively. The initial remission rate was 84% (n = 42/50). Initial non-remission occurred in 8 patients (16%), with three macro- and five microadenomas. Three (2 micro, 1 macro) of the 42 patients with initial remission had late (>50 months) recurrence of hypercortisolism that required repeat TSS. Seven patients (16.6%) with non-reduced postoperative cortisol levels showed a subsequent remission. Male gender was the only factor significantly associated with lower remission rates in either short- or long-term follow-up (p = 0.003 and 0.038 respectively).Conclusions: A team-based approach, in a specialized pituitary centre, can lead to initial and durable long-term remission in patients with CD. Additionally, we found that the risk of relapse in patients with CD persisting for more than 5 years after surgery is real but low. Moreover, non-initially reduced postoperative cortisol levels do not preclude a subsequent remission. Lifelong follow-up for patients with CD appears essential, for all patients who have shown recovery of their axis relationship.
A closer look at the transition in environmental citizenship perception among K-12 students
Authors: Oral Hasan Volkan , Eren Ozge , Yay A.Suna Erses , Cermik Erkan , Kara Hazal Aylin , Saygin Hasan 
Published: June 2022

Abstract
By evaluating the transition from higher education institutions to universities, this study attempts to examine high school students' environmental attitudes and activities. A total of 296 students from Hisar School, a high school in Istanbul, Marmara Region, Turkey, participated in the current study. The samples of higher education institutions used in this analysis were taken from the authors' prior research. To verify the given hypothesis, the same statistical analysis methods are applied to all samples, and the data is analyzed using the following statistical methods: principal component analysis, independent sample t-test, chi-square test, and regression analysis. Significant findings comprise highly derived environmental thoughts and behaviors as well as statistically agreed-upon differences between female and male students. Aside from one aspect, female students participate in more environmentally conscious thinking and activity. Furthermore, it has been discovered that environmental concerns are more prevalent among high school students. Regression analysis was employed to investigate the links between environmental thoughts and practices, and the results indicated that environmental concerns have a considerably greater impact on environmental behavior than environmental knowledge and attitude.
Challenges and Opportunities of Big data and IoT in the Electronic Banking Industry: A Systematic Literature Review
Authors: Ebrahimi Parvaneh , Moghaddam Davood Karimzadgan , Mehrabani Yavar Safaei Safaei 
Published: June 2022

Abstract
The banking industry is one of the most complex and sensitive industries that undergoes significant changes daily. Most financial institutions and banks strive to diversify and innovate payment processes, making them more challenging and secure so that they can improve their digital skills .Understanding customer behavior has become a key factor in market success, which is why the Internet of Things (IoT) can be the best way to solve the problem of collecting and sharing data over the Internet, among different objects. Based on these concepts, the purpose of this paper is to show the current perspective of the e-banking industry with big data and the IoT, as well as the impact of big data on various financial and banking sectors, especially its impact on financial markets and banking, financial institutions, Internet credit services companies, risk analysis and so on. Since big data in the field of electronic banking and finance is a completely new concept, future research opportunities will be mentioned at the end of this study.
Reliable assessment of telomere maintenance mechanisms in neuroblastoma
Authors: Meeser Alina , Bartenhagen Christoph , Werr Lisa , Hellmann Anna-Maria , Kahlert Yvonne , Hemstedt Nadine , Nürnberg Peter , Altmüller Janine , Ackermann Sandra , Hero Barbara , Simon Thorsten , Peifer Martin , Fischer Matthias , Rosswog Carolina 
Published: June 2022

Abstract
Background:
Telomere maintenance mechanisms (TMM) are a hallmark of high-risk neuroblastoma, and are conferred by activation of telomerase or alternative lengthening of telomeres (ALT). However, detection of TMM is not yet part of the clinical routine, and consensus on TMM detection, especially on ALT assessment, remains to be achieved.
Methods:
Whole genome sequencing (WGS) data of 68 primary neuroblastoma samples were analyzed. Telomere length was calculated from WGS data or by telomere restriction fragment analysis (n = 39). ALT was assessed by C-circle assay (CCA, n = 67) and detection of ALT-associated PML nuclear bodies (APB) by combined fluorescence in situ hybridization and immunofluorescence staining (n = 68). RNA sequencing was performed (n = 64) to determine expression of TERT and telomeric long non-coding RNA (TERRA). Telomerase activity was examined by telomerase repeat amplification protocol (TRAP, n = 15).
Results:
Tumors were considered as telomerase-positive if they harbored a TERT rearrangement, MYCN amplification or high TERT expression (45.6%, 31/68), and ALT-positive if they were positive for APB and CCA (19.1%,13/68). If all these markers were absent, tumors were considered TMM-negative (25.0%, 17/68). According to these criteria, the majority of samples were classified unambiguously (89.7%, 61/68). Assessment of additional ALT-associated parameters, i.e., telomere length, ATRX mutation, TERRA expression, telomere variant repeats, and telomere insertions, clarified the TMM status of the remaining seven cases with high likelihood.
Conclusions:
Based on our results we here propose a workflow to reliably detect TMM in neuroblastoma. We show that unambiguous classification is feasible following a stepwise approach that determines both, activation of telomerase and ALT. The workflow proposed in this study can be used in clinical routine and provides a framework to systematically evaluate telomere maintenance mechanisms in risk stratification and treatment allocation of neuroblastoma patients.
The microbiota, secondary metabolites and diseases: a putative role of probiotics in prevention and treatment of cancers
Authors: Seyyedi Zahrasadat , Parchebafi Atefeh , Kashani Hamed Haddad 
Published: June 2022

Abstract
Live and nonpathogenic microorganisms are called probiotics which are proved to be beneficial for their host. They play a key role on treatment and inhibition of infectious and non-infectious diseases. Since cancer is considered the main cause of death worldwide, it is important to find some strategies for reducing the side effects of chemotherapy. One of the key characteristics of probiotics is their role in treatment and recovery of different kinds of cancers by secondary metabolites which are produced by them. Up to date, different species of probiotics affecting cancer treatment have been explored. In this review, the relationship between probiotics and treatment of different diseases like cancers are investigated in order to use these crucial microorganisms in treatment of different neoplastic diseases.
“It’s Just Another Tool on my Toolbelt” New York State Law Enforcement Officer Experiences Administering Naloxone
Authors: Lloyd Danielle , Rowe Kirsten , Leung Shu-Yin John , Pourtaher Elham , Gelberg Kitty 
Published: June 2022

Abstract
BackgroundAlthough naloxone is widely acknowledged as a life-saving intervention and a critical tool for first responders, there remains a need to explore how law enforcement officers have adapted to a shifting scope of work, in many cases counter to traditional approaches to policing. Past research has focused mainly on officer training, their abilities to administer naloxone, and to a lesser extent on their experiences and interactions working with people who use drugs (PWUD).Methods A qualitative approach was used to explore officer perspectives and behaviors surrounding responses to incidents of suspected opioid overdose. Between the months of March and September 2017, semi-structured interviews were conducted with 38 officers from 17 counties across New York state (NYS). ResultsAnalysis of in-depth interviews revealed that officers generally considered the additional responsibility of administering naloxone to have become “part of the job.” Many officers reported feeling as though they are expected to wear multiple hats, functioning as both law enforcement and medical personnel and at times juggling contradictory roles. Evolving views on drugs and drug use defined many interviews, as well as the recognition that a punitive approach to working with PWUD is not the solution, emphasizing the need for cohesive, community-wide support strategies. Notable differences in attitudes toward PWUD appeared to be influenced by an officer’s connection to someone who uses drugs and/or due to a background in emergency medical services (EMS).ConclusionLaw enforcement officers in NYS are emerging as an integral part of the continuum of care for PWUD. Our findings are capturing a time of transition as more traditional approaches to law enforcement appear to be shifting toward those prioritizing prevention and diversion. Widespread adoption of naloxone administration by law enforcement officers in NYS is a powerful example of the successful integration of a public health intervention into police work.
Comparison of operative times in primary bilateral total knee arthroplasty performed by a single surgeon
Authors: Ishii Yoshinori , Noguchi Hideo , Sato Junko , Takahashi Ikuko , Ishii Hana , Ishii Ryo , Ishii Kei , Toyabe Shin-ichi 
Published: June 2022

Abstract
Background
Prolonged operative time (OT) is associated with adverse complications after total knee arthroplasty (TKA). The purpose of this study was to determine whether preoperative factors, such as sex, age, body mass index, body weight (BW), body height, American Society of Anesthesiologists grade, tibiofemoral angle, hospital for special surgery scores, surgical side, surgical order, and calendar year of surgery, affect OT.
Methods
One hundred nineteen patients (238 knees) with osteoarthritis who underwent staged bilateral primary TKA performed by a single surgeon were evaluated. The medical records of 15 males and 104 females were retrospectively reviewed. All variables were expressed as median (interquartile range).
Results
The OT for all TKAs was 57 minutes (51, 65). The OT on the left side (59 minutes (52–67)) was longer than that on the right side (55 minutes (50–62)) (p = 0.015). Multiple regression analysis revealed that longer OT was related to BW (β = 0.488, p < 0.001), calendar year of surgery (β= −0.218, p < 0.001), and operative side (β= −0.151, p = 0.007). The Jonckheere–Terpstra test showed a trend toward decreasing OT with calendar year on the left side (p = 0.037) (surgeon’s non-dominant side), but not on the right (p = 0.795). Body height, BW, and body mass index showed weak correlations (r = 0.212, p = 0.001; r = 0.352, p < 0.001; r = 0.290, p < 0.001, respectively) with OT.
Conclusion
Patients with a large physique, and especially obesity, with an affected knee on the surgeon’s non-dominant side may require a longer OT; OT decreased over time.
Bulk and single-cell transcriptome profiling reveal tumor-specific T-based molecular classification, tumor microenvironment infiltration characterization relevant to combination therapies in lung adenocarcinoma
Authors: Luo Ziwei , Liu Xuefei , Chen Ying , Shen Lize , Qin Hui , Zha Qiongfang , Hu Feng , Wang Yali 
Published: June 2022

Abstract
Background:
In lung adenocarcinoma (LUAD), the use of small-molecule inhibitors, chemotherapy and immunotherapy has led to unprecedented survival benefits in selected patients. Considering most patients will experience a relapse within a short period of time due to single drug resistance, combination therapy is also particularly important to improve patient prognosis. Therefore, more robust biomarkers to predict responses to immunotherapy, targeted therapy, chemotherapy and rationally drug combination therapies may be helpful in clinical treatment choices.
Methods:
We defined tumor-specific T cells (TSTs) and their features (TSTGs) by single-cell RNA sequencing. We applied LASSO regression to filter out the most survival-relevant TSTGs to form the Tumor-specific T cell score (TSTS). Immunological characteristics, enriched pathways, and mutation were evaluated in high- and low TSTS groups.
Results:
We identified six clusters of T cells as TSTs in LUAD, and four most robust genes from 9 feature genes expressed only on tumor-specific T cells were screened to construct a tumor-specific T cells score (TSTS). TSTS was positively correlated with immune infiltration and angiogenesis and negatively correlated with malignant cell proliferation. Moreover, potential vascular-immune crosstalk in LUAD provides the theoretical basis for combined anti-angiogenic and immunotherapy. Lachnoclostridium affect Hyaluronidase and promotes immune infiltration in the high TSTS group through CXCL16-CXCR6 between myeloid and T cells. Noticeable, patients in high TSTS had better response to ICB and targeted therapy and patients in the low TSTS group often benefit from chemotherapy.
Conclusions:
The proposed TSTS is a promising indicator to predict immunotherapy, targeted therapy and chemotherapy responses in LUAD patients for helping clinical treatment choices.
The orchid mantis exhibits high ontogenetic colouration variety and intersexual life history differences
Authors: Zhao Xin , Liu Jing-Xin , Chen Zhanqi 
Published: June 2022

Abstract
Masquerade, the resemblance of animals to inedible or inanimate objects, exists universally throughout the animal kingdom, especially in arthropods. However, masquerade has received little attention from biologists and is often misinterpreted as mimicry by the public and even by scientists, as a consequence of the lack of systematic biological information for any masquerader. Therefore, using the orchid mantis Hymenopus coronatus (Insecta: Mantodea), a classic masquerader, as the study species, we first analysed its abundance, life cycle, microhabitats, colour morph diversity and key environmental factors that may affect its life cycle, based on 11 years (2011–2021) of field investigations. The results showed that the orchid mantis a) had an extremely low wild population abundance; b) exhibited four main colour morphs (white, pink, purple and yellow); c) did not coexist with any specific plant or flower; d) had a reproductive period from May to August and a nymph developmental period from September to April; and e) may adapt to local seasonal fluctuations of temperature and precipitation throughout its life cycle. Second, we recorded its life history, colour morph flexibility and intersexual variation under laboratory conditions. The results indicated that 1) its colour morph with high flexibility exists among and within different life stages; and 2) the two sexes can be remarkably differentiated in development, lifespan and body weight. This study is the first systematic investigation of a masquerader; its results should be useful for ecologists and evolutionary biologists to interpret the adaptation and evolution of masquerade.
Streaming Hierarchical Clustering Based on Point-Set Kernel
Authors: Han Xin , Zhu Ye , Ting Kai Ming , Zhan De-Chuan , Li Gang 
Published: June 2022

Abstract

Hierarchical clustering produces a cluster tree with different granularities. As a result, hierarchical clustering provides richer information and insight into a dataset than partitioning clustering. However, hierarchical clustering algorithms often have two weaknesses: scalability and the capacity to handle clusters of varying densities. This is because they rely on pairwise point-based similarity calculations and the similarity measure is independent of data distribution. In this paper, we aim to overcome these weaknesses and propose a novel efficient hierarchical clustering called StreaKHC that enables massive streaming data to be mined. The enabling factor is the use of a scalable point-set kernel to measure the similarity between an existing cluster in the cluster tree and a new point in the data stream. It also has an efficient mechanism to update the hierarchical structure so that a high-quality cluster tree can be maintained in real-time. Our extensive empirical evaluation shows that StreaKHC is more accurate and more efficient than existing hierarchical clustering algorithms.
Impact of Abnormal Potassium on Arrhythmia Risk During Pediatric Digoxin Therapy
Authors: Mlambo Vongai Christine , Algaze Claudia , Mak Kwai , II R. Thomas Collins 
Published: June 2022

Abstract
Background: Digoxin is used in children with heart failure and tachyarrhythmia. Its use in patients with single ventricle anatomy has increased following evidence of improved interstage survival after the Norwood procedure. Digoxin has a narrow therapeutic window and may alter serum potassium balance, inducing arrhythmias. We hypothesized digoxin use in the setting of abnormal serum potassium levels is associated with arrhythmias. Methods: We reviewed all patients ≤18 years who received digoxin while admitted at our institution from 2014-2021. Admissions <2 nights were excluded. We compared patients with a hemodynamically significant arrhythmia to those without. We performed adjusted logistic regression with arrhythmia as the outcome variable and potassium status as the predictor variable; adjusting for weight, route of digoxin administration, digoxin indication, serum creatinine, and number of interacting drugs prescribed. Abnormal potassium was defined as serum levels <3.5 mmol/L or >6.0 mmol/L.Results: There were 272 encounters in 172 patients. Potassium levels were abnormal in 72.9% of patients who experienced an arrhythmia during digoxin administration, compared to 40.3% who did not (p < 0.001). Odds of arrhythmia was 133% higher in patients with abnormal potassium receiving digoxin (AOR=2.33, 95% CI 1.16 – 4.68, p=0.02). Receiving intravenous digoxin was also associated with a 4.46 odds of cardiac arrhythmia (AOR 4.46, p=0.04, 95% CI 1.07 to 18.50).Conclusion: Odds of arrhythmia is increased during digoxin administration when pediatric patients have abnormal potassium levels. Vigilant attention to potassium levels is essential to prevent adverse outcomes during digoxin therapy.
An Artificial Intelligence Strategy for the Deployment of Future Holographic Type Communication Services in Edge-Cloud Computing
Authors: Ssemakula John Bosco , Gorricho Juan-Luis , Kibalya Godfrey 
Published: June 2022

Abstract
Given the current predictions of B5G, the maturity of 5G technology, and proposed network services that are both compute-intensive and latency-sensitive, there are different levels of criticality that require flexibility and agility in decision making to support new services on the network. The current traditional cloud computing service model cannot handle the explosive growth and demands of such use cases as HTC (Holographic Type Communication) services. The purpose of edge computing (EC) is to effectively solve problems such as minimizing latency and optimizing network utilization. However, flexibility and agility are also important requirements for cost-effective and resource-efficient deployment of such services. Considering the NP-hard nature of the problem, an artificial intelligence technique based on a reinforcement learning (RL) algorithm is proposed to make an intelligent decision on the optimal serving tier and edge-site selection, while considering criticality levels and multiple conflicting costs. In addition, at the edge network tier, a heuristic algorithm is used to map stream tasks onto the optimally selected edge-sites using a ranking mechanism based on the available network and computational resources. All algorithms aim to minimize the cost in the edge-cloud system to increase the revenue of a mobile network operator (MNO) by encouraging more people to use such outstanding future services at a lower cost. The simulation results show that the performance of the proposed RL-based algorithm is close to the optimal solution in achieving the main objective within a margin of 7%, moreover RL outperforms other benchmarks in most of the conducted experiments.
Comprehensive analysis of lncRNA–miRNA–mRNA ceRNA networks in atherosclerosis
Authors: Shi Jian , Liu Shiming , Jiang Wenyi 
Published: June 2022

Abstract
Background
Atherosclerosis (AS) is a chronic inflammatory disease characterized by lipid necrosis and vessel wall hardening manifested by intimal lipid deposition and smooth muscle cell proliferation. The interactions and mechanisms involved in the development of atherosclerotic plaques are important to understand. Growing evidence shows that long non-coding RNAs (lncRNAs) can act as competing endogenous RNAs (ceRNAs) by competitively splicing microRNAs (miRNAs) to regulate key cellular and molecular processes during various stages of AS. The aim of this study was to elucidate the potential role of lncRNAs, miRNAs and mRNA ceRNA networks in advanced atherosclerotic plaque tissue compared with normal intimal tissue.
Methods
The limma package was used to identify differentially expressed lncRNAs and mRNAs (DElncRNAs and DEmRNAs, respectively) based on expression profiles from the Gene Expression Omnibus (GEO) datasets GSE97210 and GSE137578. Differentially expressed genes (DEGs) were further analysed by Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analysis and Metascape online tools. The intersection of the two DEG datasets was screened for hub genes. In addition, a lncRNA–miRNA–mRNA–Hub gene regulatory network was constructed to investigate key genes that may influence AS pathogenesis.
Results
A total of 393 probes associated with DElncRNA, including 122 up-regulated and 271 down-regulated probes were identified from the GSE97210 dataset, and 675 up-regulated and 689 down-regulated DEGs were also identified in atherosclerotic samples. In the validation dataset (GSE137578), 400 up-regulated and 388 down-regulated DEGs in atherosclerotic samples were identified. CeRNA networks were constructed based on the expression profiles of lncRNAs, miRNAs and mRNAs in patients with AS, and a total of 80 lncRNA nodes, 30 miRNA nodes, 80 mRNA nodes and 625 edges were identified as differential expression profiles. Four core genes, including BAMBI, TBX18, DLGAP5 and RRM2, were screened after taking intersections with the validation dataset. Then, a ceRNA regulatory relationship network consisting of these four mRNAs, three miRNAs and 13 lncRNAs was established.
Conclusion
Our study found that BAMBI, TBX18, DLGAP5 and RRM2 are potentially involved in the pathological process of AS.