bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global datasets of leaf photosynthetic capacity for ecological and earth system research
Authors: Chen Jing M. , Wang Rong , Liu Yihong , He Liming , Croft Holly , Luo Xiangzhong , Wang Han , Smith Nicholas G. , Keenan Trevor F. , Prentice I. Colin , Zhang Yongguang , Ju Weimin , Dong Ning 
Published: September 2022

Abstract. The maximum rate of Rubisco carboxylation (Vcmax)
determines leaf photosynthetic capacity and is a key parameter for
estimating the terrestrial carbon cycle, but its spatial information is
lacking, hindering global ecological research. Here, we convert leaf
chlorophyll content (LCC) retrieved from satellite data to Vcmax, based
on plants' optimal distribution of nitrogen between light harvesting and
carboxylation pathways. We also derive Vcmax from satellite (GOME-2)
observations of sun-induced chlorophyll fluorescence (SIF) as a proxy of
leaf photosynthesis using a data assimilation technique. These two
independent global Vcmax products agree well (r2=0.79,RMSE=15.46µmol m−2 s−1, P<0.001) and compare well
with 3672 ground-based measurements (r2=0.69,RMSE=13.8µmol m−2 s−1 and P<0.001 for SIF; r2=0.55,RMSE=18.28µmol m−2 s−1 and P<0.001 for LCC). The LCC-derived
Vcmax product is also used to constrain the retrieval of Vcmax from TROPical Ozone Mission (TROPOMI) SIF data to produce an optimized Vcmax product using
both SIF and LCC information. The global distributions of these products are
compatible with Vcmax computed from an ecological optimality theory
using meteorological variables, but importantly reveal additional
information on the influence of land cover, irrigation, soil pH, and leaf
nitrogen on leaf photosynthetic capacity. These satellite-based approaches
and spatial Vcmax products are primed to play a major role in global
ecosystem research. The three remote sensing Vcmax products based on
SIF, LCC, and SIF+LCC are available at https://doi.org/10.5281/zenodo.6466968 (Chen et al., 2022), and the code
for implementing the ecological optimality theory is available at https://github.com/SmithEcophysLab/optimal_vcmax_R and https://doi.org/10.5281/zenodo.5899564 (last access: 31 August 2022) (Smith et al., 2022).
A new operational Mediterranean diurnal optimally interpolated sea surface temperature product within the Copernicus Marine Service
Authors: Pisano Andrea , Ciani Daniele , Marullo Salvatore , Santoleri Rosalia , Buongiorno Nardelli Bruno 
Published: September 2022

Abstract. Within the Copernicus Marine Service, a new operational MEDiterranean diurnal optimally interpolated sea surface temperature (MED DOISST) product has been developed. This product provides hourly mean maps (level 4) of subskin SST at 1/16∘ horizontal resolution over the Mediterranean Sea from January 2019 to the present. Subskin is the temperature at ∼1 mm depth of the ocean surface and then potentially subjected to a large diurnal cycle. The product is built by combining hourly SST data from the Spinning Enhanced Visible and InfraRed Imager (SEVIRI) on board the Meteosat Second Generation and model analyses from the Mediterranean Forecasting System (MedFS) through optimal interpolation. SEVIRI and MedFS (first layer) SST data are respectively used as the observation source and first guess. The choice of using a model output as first guess represents an innovative alternative to the commonly adopted climatologies or previous day analyses, providing physically consistent estimates of hourly SSTs. The accuracy of the MED DOISST product is assessed here by comparison against surface drifting buoy measurements covering the years 2019 and 2020. The diurnal cycle reconstructed from DOISST is in good agreement with the one observed by independent drifter data, with a mean bias of 0.041±0.001 K and root mean square difference (RMSD) of 0.412±0.001 K. The new SST product is more accurate than the input MedFS SST during the central warming hours, when the model, on average, underestimates drifter SST by 1/10 of a degree. The capability of DOISST to reconstruct diurnal warming events, which may reach intense amplitudes larger than 5 K in the Mediterranean Sea, is also analyzed. Specifically, a comparison with the Operational Sea Surface Temperature and Sea Ice Analysis (OSTIA) diurnal skin SST product, SEVIRI, MedFS, and drifter data shows that the DOISST product is able to reproduce more accurately diurnal warming events larger than 1 K. This product can contribute to improving the prediction capability of numerical models that assimilate or correct the heat fluxes starting from level 4 SST data and the monitoring of surface heat budget estimates and temperature extremes which can have significant impacts on the marine ecosystem. The full MED DOISST product (released on 4 May 2021) is available upon free
registration at https://doi.org/10.48670/moi-00170 (CNR, 2021). The reduced subset used here for validation and review purposes is openly available at https://doi.org/10.5281/zenodo.5807729 (Pisano, 2021).
SISTEM PENDUKUNG KEPUTUSAN PENERIMA BANTUAN SOSIAL TUNAI DENGAN METODE TECHNIQUE FOR ORDER PREFERENCE BY SIMILARITY TO IDEAL SOLUTION
Authors: Ade Solihin Sopandi  , Dudih Gustian  , Sembiring Falentino , Muhamad Muslih  , Nunik Destria Arianti  , Aan Setiawatif  , Kurniawan  
Published: September 2022

Pemerintah telah berupaya menangani kemiskinan di masa pandemi Covid-19 dengan salah satu programnya yang dinamakan Bantuan Sosial Tunai nominal Rp 600.000 per bulan yang sasaran utamanya yaitu mereka yang paling terdampak. Akan tetapi program tersebut ternyata menemukan keganjilan sehingga mekanisme program tersebut tidak sesuai dengan yang diharapkan oleh semua pihak, kurang tepatnya sasaran penerima bantuan menjadi masalah serius yang dihadapi pemerintah dalam hal ini adalah pihak-pihak RT dan Desa yang menjadi acuan dasar data-data calon penerima bantuan. Oleh karena itu perlu dibuatkan sistem pendukung keputusan yang dapat membantu memecahkan masalah tersebut, Metode Technique for Order Preference by Similarity to Ideal Solution dipandang mampu untuk menanggulangi masalah penerima bantuan yang kurang tepat sasaran. Penelitian ini menggunakan metode TOPSIS agar penilaian akan lebih tepat sasaran dalam proses penyaluran bantuan. Dalam penelitian ini ditentukan lima kriteria yang telah ditentukan bobot nilai kriterianya yaitu Pekerjaan 20%, Penghasilan 25%, Usia 25%, Jumlah Tanggungan 15% serta Jenis Rumah 15%. Dari hasil penelitian dan perhitungan yang telah dilakukan ditemukan hasil perbandingan antara perhitungan manual dengan perhitungan melalui sistem dengan nilai akurasi sekitar 94,96%. Sistem pendukung keputusan calon penerima Bantuan Sosial Tunai (BST) Covid-19 ini diharapkan menjadi solusi dari dampak dari masalah kurang objektifnya penerima bantuan tersebut serta dapat membantu pihak terkait dalam menentukan penerima bantuan agar lebih praktis, objektif dan tepat sasaran.
Efektivitas Penghambatan Ekstrak Tumbuhan Obat Lokal Terhadap Pertumbuhan Colletotrichum Gloeosporioides Penyebab Penyakit Bercak Daun Alpukat
Authors: Supriati Lilies , Widayanti Mulyati , Djaya Adrianson Agus , Mulyani Rahmawati Budi , Anwar Mochammad 
Published: September 2022

Penyakit bercak daun alpukat (Colletotrichum gloeosporioides) sangat merugikan, serangan penyakit terjadi pada daun, ranting, bunga dan buah hingga ke penyimpanan dan pemasaran. Pengendalian penyakit tanaman yang bersifat ramah lingkungan dan aman bagi kesehatan dapat dilakukan menggunakan tumbuhan obat lokal, namun informasi penelitian tentang hal ini belum banyak. Penelitian bertujuan untuk engetahui mengetahui efektivitas penghambatan tumbuhan obat lokal yang efektif menekan pertumbuhan jamur patogen C. gloeosporioides penyebab penyakit bercak daun tanaman alpukat secara in vitro. Perlakuan terdiri dari 4 taraf konsentrasi ekstrak tumbuhan obat lokal yaitu: P0 (kontrol tanpa ekstrak), P1 (ekstrak pasak bumi 5%), P2 (ekstrak pasak bumi 10%), P3 (ekstrak pasak bumi 15%), P4 (ekstrak pasak bumi 20%), P5 (ekstrak akar kuning 5%), P6 (ekstrak akar kuning 10%), P7 (ekstrak akar kuning 15%), P8 (ekstrak akar kuning 20%), P9 (ekstrak umbi hati tanah 5%), P10 (ekstrak umbi hati tanah 10%), P11 (ekstrak umbi hati tanah 15%), P12 (ekstrak umbi hati tanah 20%), P13 (ekstrak umbi sarang semut 5%), P14 (ekstrak umbi sarang semut 10%), P15 (ekstrak umbi sarang semut 15%) dan P16 (ekstrak umbi sarang semut 20%). Hasil penelitian menunjukkan ekstrak akar pasak bumi pada taraf konsentrasi 20% sangat efektif menghambat pertumbuhan diameter koloni jamur C. gloeosporioides dengan efektivitas penghambatan 94.4%, dan efektif menghambat perkecambahan spora sebesar 6.81%.
Significance of Incorporating Language Skills into EFL Syllabus
Authors: Abdalla Al-Sadig Yahya 
Published: September 2022

It is generally observed that most Arab EFL learners (and some teachers) demonstrate poor communication skills. Other things being equal, this paper draws on the assumption that part of the problem lies with the kind of training that the English departments have been offering; viz. the bulk of their courses centres upon the teaching of knowledge course: literature, pure linguistics and English language rules. These, the paper argues, could hardly improve learners’ skills at listening, reading, speaking and writing. This paper attempts to examine this assumption in the light of the existing EFL syllabus in some Arab universities. In so doing, it begins by giving a theoretical overview of the major theories shaping modern English language teaching methods and proceeds to evaluate the Arab EFL syllabus in the light of these theories.
of into EFL
كَسْرُ البِنَاءِ اللُّغوي دِرَاَسَةٌ في أُسْلُوبِ المُطَابَقَةِ عِنْدَ المُتَنَبِي
Authors: آدم  الصادق محمد 
Published: September 2022

يَتَنَاوَلُ هذا البَحْثُ كَسْرَ البِنَاءِ اللّغوي باعتباره ظاهرةًًً تعتمدُ على الخروجِ على النّمطِ المِثالي للأداء، وهي دِراسةٌ تستمدُ مظاهِرَها في شِعْرِ أبي الطّيب المتنبي ( 303– 354هـ ، 915- 965م) الذي انْحَرَف كثيراً في أساليبه عن النّمط المعهود بحثاً عن تركيبٍ غيرِ عاديٍ، وذلك لغاياتٍ فنيةٍ وجماليةٍ، كسراً يسببُ للمتلقي هِزّةً غير متوقَّّعةٍ، فالمتنبي عند اسْتِعْمَالِه الفنّيّ للّغة يَمِيْلُ ميلاًً شديداً نحو كَسْرِ رَتَابِة النّظامِ اللُّغوي المُعْتَادِ على عدةِ مستوياتٍ.
وهذا الانحرافُ في الأسلوبِ هو السببُ الأوّلُ الذي جعل النّقادَ يُعْنَونَ بشِعْرِه في مُخْتَلفِ العصور إعجاباً بالطّريقة التي عالج بها بعض النّصوص، لأنّ ما يهمّ الناقد في النّصّ الشّعري لا يتوقف على ما يَقُوله النّصُ، وإنّما الطّريقةُ التي يُقالُ بها النّصُ أيضاً.
والمنهجيةُ الّتي اتبعتُها هي الاستقراءُ ثُمَّ التَّحليلُ، وذلك بمدارسةِ الديوان واستخراجِ ما فيه من أساليب لم تتفقْ نَمَطُها مع القواعدِ الأساسيةِ إلي تتبعها اللُّغة.
والجديد ُفي هذه الدراسةِ هو تبريرُ هذا الانحراف اللّغوي على أنَّه تشكيلٌ للغة حسبما تقتضيه حاجةُ الشَّاعر النَّفسية ، وبالطَّريقة التي يراها أكثر تأثيراً للمتلقي، لإحداثِ عُنْصُرِ الإحْساسِ بالدهشةِ والمفاجأةِ.
أما النتائجُ المُتَوَصَّل إليها فهي أنّ المتنبي في سلوكه هذا المسلك لا يمكن أنْ تُوصف لغته بالضّعفِ أو يُوصف هو بالعَجْزِ عن الأخذِ بناصيتها ، بل هو دليلٌ لجُرْأَتِه وتَصَرُّفِه ، إذ أنّه يُريدُ أن يُعَبَّر عن شيء في نَفْسِه لا تُؤَدِيه اللّغةُ العاديةُ فَتَصَرَّفَ في أدَاتِه اللّغويةِ بِوَعِيٍ.
نشأة الكتابة العربية وتطورها من العصر الجاهلية إلى صدر اإلسالم
Authors: رحماني عبد الرزاق 
Published: September 2022

ناقشت هذه الدراسة اختلاف المؤرخين حول نشأة الكتابة العربية، ففريق يرى أن نشأتها كانت إلهية محضة، حيث إن الله عز وجل قد أوحى إلى آدم بطريقة الكتابات كلها ثم كتب بها آدم كل الكتب، بينما يذهب فريق آخر إلى أن الكتابة العربية اشتقت من الكتابة النبطية.
كما أعطيت تعريفاً للكتابة لغة واصطلاحاً، ومراحل تطورها، ولقد مرَّ الكتابة بأطوار عدة قبل أن تصل إلى الطور الهجائي المستخدم في أيامنا؛ وركزت الدراسة على أدوات الكتابة وموضوعاتها.
كما اهتمت الدراسة بالكتابة في عهد النبي والخلفاء الراشدين؛ واهتمام القرآن الكريم بالكتابة وأدواتها.
ويتبع الباحث المنهج التاريخي و التحليلي للتوصل إلى معرفة الحقائق العلمية والثقافية في الكتابة العربية.
نحو موازنة سنوية لمكتبة جامعة الخرطوم
Authors: عبد الرحمن  عمر حسن 
Published: September 2022

هدفت هذه الورقة إلى دراسة واقع التمويل بمكتبة جامعة الخرطوم الرئيسة وفروعها , وخاصة فى جانب الموازنة السنوية , وذلك على ضوء المعايير العالمية المعمول بها فى هذا الشأن. عرفت هذه الدراسة أنواع الموازنات المختلفة المعمول بها فى المكتبات الجامعية ثم وضحت دور أمين المكتبة فى تقدير وإعداد الموازنة السنوية. كذلك استعرضت الورقة النسب المعيارية العالمية المعمول بها فى تخصيص موازنة للمكتبات الجامعية من المؤسسة الأم. ثم استعرضت المصادر الأخري لدعم موازنة المكتبات وخاصة سياسة جمع الأموال والتبرعات Fundraising التى أصبحت من النشاطات المهمة فى هذا المجال. تناولت الورقة جانب إدارة الموازنة بالتركيز على طريقة الصيغة فى توزيع موازنة التزويد بمصادر المعلومات على الكليات والأقسام الأكاديمية المختلفة بالجامعة. كشفت الورقة عن عدم تخصيص موازنة سنوية لمكتبة جامعة الخرطوم خلال فترة العشرين عاما الماضية. وأخيرا وضعت الورقة مجموعة من التوصيات لمعالجة مشكلة التمويل والموازنة السنوية لمكتبة جامعة الخرطوم حتى تتمكن من أداء وظيفتها الاساسية فى توفير خدمات المعلومات لمجنمع الجامعة.
استطاع و اسطاع دراسة في المبنى والمعنى
Authors: القحطاني  فهد بن سعيد آل مثيب 
Published: September 2022

يقوم هذا البحث على دراسة الفعل " استطاع "بصورتيه: ببقاء التاء وبحذفها "استطاع ، اسطاع" دراسة في المبنى ، تكشف عن أصل بناء الفعل بصورتيه ، ولغات كل صورة ، وأوزانها ، مع بيان اللغات التي تصح قراءة القرآن بها ،و اللغات التي لا تصح قراءته بها .
ودراسة دلالية تكشف عن الفارق الدلالي بين صورة الفعل ببقاء التاء" استطاع " وصورته بحذف التاء منه " اسطاع" مستقصية أقوال المفسرين واللغويين ، ثم خلصت إلى بيان القول الراجح وفق الأدلة العلمية .
الشعر والدين والأخلاق بين النقاد والفلاسفة
Authors: دياب ألاء ياسين   
Published: September 2022

هدفت هذه الدراسة إلى إظهار أثر الجانب الأخلاقي و الدّيني في النقد العربي القديم من خلال تتبعه عند النقاد والفلاسفة، الّذين انقسمت مواقفهم بين أولئك الّذين ربطوا بين الشّعر من جهة، والدّين والأخلاق من جهة أخرى، فطالبوا الشّاعر بضرورة الالتزام بتعاليم الدين وبمعايير الأخلاق. وبين أولئك الذين فصلوا بين الشّعر و الدّين والأخلاق، وحكموا على الشّعر بمقايي س موضوعيّ ة ، فتنوّعت مواقفُ النّقاد والفلاسفة بين متشدد و متساهل و معتدل.
انفتاح اللغة العربية المعاصرة
Authors: عمر زكي عثمان عبد المطلب 
Published: September 2022

This paper deals with the study and analysis of a phenomenon that has emerged - to a certain extent – in the contemporary Arabic Language, that is: It became gathering new types of the Feminine sound Plural that were not touched by the rules of the elders so that the abstract’s title was adopted. The study began with the debate that took place among the elders in favoring the term: "feminine sound plural" to the term: "plural ending with ((Alphabetical Letters: Alif (A) and Ta’a (T))), after that they made comparison between the strict conditions of the Sound muscular plural and the Non-strict conditions of the Sound Feminine Plural. Then, the Paper presented the varieties being recorded and grouped by Elders as Sound Feminine Plural together with exceptional words thereof at a limited extent. The Paper then went on to reveal the new species that the Contemporary Arabic has opened up to and have been applied by the Sound Feminine Plural group in aremarkable abundance. The Paper categorized the new types which the contemporary Arabic has opened up to, into three Classes comprised:Class (1) the masculine Words, either names of solids or Derivatives shifted to the nominal field.Class (2) the infinitives including the (3-lettered Verb) at a limited extent, and the infinitives of the (4-lettered Verbs) to a wider extent thereof, but the remarkable openness being onto the infinitives of 5-& 6- lettered verbs.Class (3) the Foreign Words which includes the terminologies word and the other exotic words implanted in the Arabic Language whereas they have been interested to apply the form of the Sound Feminine Plural for its simplicity as it require to only add (Alphabetical Letters: Alif (A) and Taa (T)) to the end of the nominal words.
Pengaruh Kompetensi Sumber Daya Manusia Terhadap Kualitas Laporan Keuangan Pemerintah Daerah Kabupaten Halmahera Utara
Authors: Mitjo Fenti , Kawatu Freddy Semuel , Tangkau Jaqueline 
Published: September 2022

Laporan keuangan merupakan salah satu informasi yang sangat penting dalam suatu entitas yang menggambarkan posisi keuangan pada periode tertentu serta perkembangan atau kemajuan dari entitas tersebut yang sangat membantu dalam proses pengambilan keputusan. Tujuan dari penelitian ini untuk mengetahui seberapa besar pengaruh kompetensi sumber daya manusia terhadap kualitas laporan keuangan pemerintah daerah kabupaten halmahera Utara. Metode penelitian yang digunakan adalah metode penelitian kuantitatif. Penelitian ini menggunakan kuesioner sebagai instrumen. Populasi dalam penelitian ini adalah seluruh pegawai SKPD kabupaten halmahera Utara yang berjumlah 37 responden. Pada penelitian ini teknik pengambilan sampel dilakukan secara purposive sampling dengan menggunakan rumus Slovin, dan sampel yang didapatkan dan digunakan berjumlah 20 responden. Analisis data yang di gunakan dalam penelitian ini adalah Correlation Spearman Rho dengan bantuan komputer melalui aplikasi SPSS. Dari perhitungan yang didapatkan kompetensi sumber daya manusia berpengaruh signifikan dengan kualitas laporan keuangan SKPD kabupaten halmahera Utara dengan tingkat hubungan kuat dan positif.
Minimum relevant features to obtain AI explainable system for predicting breast cancer in WDBC
Authors: Rashi Agarwal , Madamala Revanth 
Published: September 2022

The potential to explain why a machine learning model produces a certain prediction in incomprehensible terms is becoming increasingly crucial, as it provides accountability and confidence in the algorithm's decision-making process. The interpretation of complex models is difficult. Various approaches to dealing with this issue are being offered. These problems are typically handled in tree ensemble methods by assigning priority levels to input features globally or for a specific prediction. We show that current feature attribution approaches are inconclusive, and develop solutions using SHAP (SHapley Additive Explanation) values, LIME (Local Interpretable Model-Agnostic Explanations), and the Skope Rules package. We employ feature selection methods from SHAP and LIME in this work, which uses the Breast cancer Wisconsin data sets. In the suggested method, features are chosen at the first level of feature selection using Decision tree entropy values. Based on the SHAP and LIME reports, level 2 features are chosen from fewer options. The features are tested on a Decision Tree (DT) model and a DT and Support Vector Machine (SVM) ensemble. Experiments suggest that the ensemble works better as compared to DT. We have also used the Skope Rules package to generate global rules for generalization.
Moderat Islamic Da’wah to Eliminate Islamophobia
Authors: Sila Muhammad Amin 
Published: September 2022

Islamophobia is one of the latent problems in many moslem country, including where the majority of the population is Muslim, such as Indonesia. Hatred and fear of Islam, can disturb the harmony in society. This article describes the dangers of Islamophobia, and some strategies which can be taken to handle it. The aims of this study are to explore what are the challenges and opportunities of the Indonesian people regarding these Islamophobia problems. This article uses the theory and concept of Islamophobia. In addition, the concept of discourse popularized by Jurgen Habermas is used to examine the problems. This qualitative study uses a literature approach. Based on the discussion conducted, Islamophobia in Indonesia can permeate the personalities of non-Muslims and Muslims alike. Therefore, efforts are needed to spread an understanding of the true teachings of Islam. Such efforts are the responsibility of all Muslims. One of the ways that can be taken, is echoing Islam rahmatan lil'alamin, spoken and written at every opportunity. Most importantly, practice behavior according to the noble values ​​of Islam in order to represent the goodness of Islam in front of the widest public.
Keywords: Indonesia, Islam, Islamophobia, compassion, discourse
Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis)
Authors: Mishra Girish Kumar , Kumar Manas Patra , Singh Laishram Kipjen , Upmanyu Vikramaditya , Chakravarti Soumendu , Karikalan Mathesh , Panigrahi Manjit , Singh Sanjay Kumar , Das Goutam Kumar , Narayanan Krishnaswamy , Kumar Harendra 
Published: September 2022

In the past decade, kisspeptin research was primarily focussed on the regulation of GnRH release in hypothalamus. Present study was designed to explore the expression of extra-hypothalamic kisspeptinergic (Kiss1- Kiss1r) system in the follicular compartment of buffalo ovary. Buffalo genitalia (n=32) were collected immediately after exsanguinations and categorized into early luteal (EL), mid luteal (ML), follicular (FL) and acyclic (n=8 per group), based on the gross ovarian morphology. Ovarian follicular tissue samples were subjected to total RNA extraction, cDNA synthesis and qPCR amplification of Kiss1, Kiss1r, follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) along with an endogenous control (β-actin) gene. The expression of ovarian Kiss1 transcripts was abundant in the cyclic than acyclic stage. The fold change was significantly upregulated in ML (66.79 fold) followed by EL (28.64 fold) and FL (14.09 fold) stages against the acyclic stage (calibrator). Similarly, the Kiss1r expression was highest at ML (77.26 fold). The expression of FSHR was upregulated at FL (15.08 fold) stage in response to follicular activity and subsequently observed to be down regulated at EL (0.09 times) and ML (0.27 times). Further, the expression of Kiss1 was positively correlated with FSHR only at ML and FL. From this study, it could be concluded that Kiss1 and Kiss1r are expressed in the buffalo ovarian follicle and their expression is associated with the stage of estrous cycle.
Phylogenetic exploration and haemato-biochemical significance of Anaplasma marginale infection in water buffalo (Bubalus bubalis)
Authors: Ganguly Anita , Maharana Biswa Ranjan , Kumar Ankit 
Published: September 2022

During 2017-2020, a total of 8352 buffalo blood samples were screened microscopically for Anaplsma species and 104 positive samples were found. Later on same samples were confirmed positive using genomic DNA PCR technique. For efficiency of PCR technique, a total of 335 blood samples were randomly chosen. Microscopy revealed Anaplasma species in erythrocytes of 1.19% (4 samples) of the total blood samples (n=335). Initially PCR assay was standardized targeting 16S rRNA for detection of genus Anaplasma with expected amplicon size 781 bp. Thereafter, msp1 Î² gene was targeted for species level identification of Anaplasma (Anaplasma marginale) with expected amplicon size 407 bp. A total of fourteen samples out of 335 samples (4.17%) found positive by PCR. Randomly, two confirmed positive samples were sent for bidirectional sequencing. The sequencing results, submitted in GenBank(MW269530, MW269531), further confirm the Anaplasma marginale infection in buffalo. The study show higher specificity and sensitivity of PCR test above blood smear examination.Â Erythron of infected animals showed significantly low level of mean Hb, PCV, RBC count, MCH, MCHC and high level of mean MCV, indicating regenerative hypochromic macrocytic anaemia. Infected animals had a significantly low mean level of total protein, albumin and globulin and significantly high level of phosphorus, total bilirubin, direct and indirect bilirubin. The current study provides indication of the association between A. marginale infection and probable liver damage or hepatic fatty degeneration. Nevertheless advanced studies are warranted to explain the precise pathophysiological mechanism of this association.