bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Lifted Veil of Uncommon EGFR Mutation p.L747P in Non-Small Cell Lung Cancer: Molecular Feature and Targeting Sensitivity to Tyrosine Kinase Inhibitors
Authors: Yang Guangjian , Liu Chengming , Hu Jiaqi , Sun Yang , Hu Peizeng , Liu Liu , Xu Haiyan , Li Dazhou , Li Weihua , Yang Yaning , Sun Nan , He Jie , Wang Yan 
Published: February 2022

ObjectivesThe uncommon p.L747P mutation in epidermal growth factor receptor (EGFR) exon 19 reveals to alter the response to tyrosine kinase inhibitors (TKIs) in patients diagnosed with advanced non-small cell lung cancer (NSCLC). However, the underlying mechanism is still not clear. This study aimed to investigate the clinical outcomes, binding affinities, and modes of action of currently available EGFR TKIs towards p.L747P mutation.Materials and MethodsClinical data of NSCLC patients harboring p.L747P mutation who had received different generations of EGFR TKIs were collected from medical records. Computational structure of p.L747P was constructed and in vitro cellular kinase inhibition assay and mice xenograft experiment were performed to predict and confirm the binding affinities and antitumor activities of diverse EGFR TKIs.ResultsA total of five metastatic NSCLC patients with p.L747P mutation were included in the final analysis. Patients treated with second-generation (2G) TKI afatinib achieved numerically longer progression-free survival (range 2.4-8.5 months) than that with first-generation (1G, range 1.4-5.5 months) or third-generation (3G, range 1.6-7.5 months) TKIs. None of the patients administered 1G or 3G TKIs achieved tumor response, but two-thirds of them treated with afatinib achieved partial response. Dynamics simulation predicted that 2G TKIs presented the best binding affinity to p.L747P mutation. The cellular kinase inhibition assay and mice xenograft experiment confirmed that afatinib could potently inhibit p.L747P-mutant cells and significantly reduce p.L747P-mutant tumor growth (P&lt; 0.001), together with reduced phosphorylation of EGFR and its downstream signalings.ConclusionsThe uncommon p.L747P mutation in EGFR exon 19 resulted in a poor response to first-generation EGFR TKIs. Afatinib revealed a better clinical response and binding affinity compared with osimertinib for this specific alteration.
Prevalence of Visual Impairment Among Students Before and During the COVID-19 Pandemic, Findings From 1,057,061 Individuals in Guangzhou, Southern China
Authors: Liang Jing-hong , Chen Yi-can , Zhao Yu , Kakaer Aerziguli , Jiang Nan , Huang Shan , Zhang Shu-xin , Chen Ya-jun 
Published: February 2022

BackgroundVisual impairment (VI) is a growing public health concern among students as a result of the COVID-19 pandemic.ObjectiveThis study investigated the change in VI before and during the pandemic among students.MethodsData on 547,864 and 497,371 students were obtained from the Guangzhou Survey on Students' Constitution and Health (GSSCH) collected in October 2019 and October 2020, respectively. VI was defined as the unaided distance visual acuity lower than 20/25 Snellen equivalent (LogMAR 0.10) in the worse eye. Change in VI based on age and sociodemographic variables were evaluated by chi-square test for trend as appropriate. Comparisons of different categorical variables were tested by contingency tables-based chi-square test. We have further analysis of the students who went through both of the 2019 and 2020 examinations for evaluating the VI incidence during the COVID-19 pandemic.ResultsMore than 1,045,235 students were involved in our study, among whom 271,790 (54.65%) out of 497,371 students in Guangzhou suffered from VI during the COVID-19 compared with 293,001(53.48%) visually impaired students (total tested participants = 547,864) before the COVID-19 pandemic. During the pandemic, the overall prevalence of VI actually showed an increased age tendency and reached the highest level in the 17 [80.04%, 95%Confidence interval (CI):79.53 to 80.54%] and the 18 (79.64, 95%CI: 79.06 to 80.23%) age groups. Rapid growth was detected among students aged between 9 and 16 years old (raised by 46.21) while older students were more likely to get moderate and severe VI than younger ones. Students involved in more screen-based activities [(64.83%, 2019); (66.59%, 2020)] appeared to have a higher prevalence of VI than those involved in less [(49.17%, 2019); (49.26%, 2020)].ConclusionA rising trend of VI among students was detected before and during the COVID-19 pandemic. Moreover, the pandemic appeared to be associated with a rapid VI shift in younger and boy populations. Potential danger may arise when public health emergencies occur in the school, and more effort should be made to improve students' vision.
Inflammatory Related Reactions in Humans and in Canine Breast Cancers, A Spontaneous Animal Model of Disease
Authors: Tricarico Domenico , Convertino Anna Sara , Mehmeti Irsida , Ranieri Girolamo , Leonetti Francesco , Laface Carmelo , Zizzo Nicola 
Published: February 2022

Inflammatory cells are emerging markers in various cancers in human trials. The relationship between the inflammatory cells response, cancer grade, and progression has been investigated experimentally in a spontaneous canine model of breast cancer and in the unselected population (18–64 years.o.) under anti-HER2 treatments that represent the most prevalent population in this cancer type. The canine data (N samples = 101) were collected retrospectively for diagnosis in our regional area and evaluated by immunohistochemistry and haemato-chemistry. The inflammatory and immune-related adverse reactions (ADR) in humans were evaluated using EudraVigilance. The “Proportional Reporting Ratio” (PRR) of the mabs was calculated for each ADR with values &gt;2 indicative of high risk. In dogs, we found elevated immunostaining of CD68-macrophages in the lymph node of the aggressive cancer G3 and infiltrating CD20+-lymphocyte. A high density of CD20 + lymphocytes was observed in G1 and a decrease in the density was observed with the histological degree of the tumors. The animals with the sample in G1 showed reduced serum platelet and neutrophil count and elevated lymphocytes and the opposite in severely affected animals. Inflammatory reactions with edema, skin reactions, extravasation, loss of effectiveness, and platelet count decrease (PRR &gt; 13) were found with trastuzumab emtansine in humans, in the absence of immune system reactions. Trastuzumab i.v.-s.c. showed immune system reactions, loss of effectiveness, intolerances with drug withdrawal, technological issues (PRR &gt; 7), and neutrophil count decrease reports. These reactions were less frequently reported for pertuzumab i.v. Case reports of platelet and neutrophil count decrease were not associated with disease progression with a better outcome in humans as in canine breast cancer. Therefore, infiltrating CD68-macrophages are associated with G3, while infiltrating CD20+ and elevated serum lymphocytes in parallel with reduced platelet and neutrophil count play a favorable role in human and canine breast cancer.
Relationship Between Inflow Impairment and Skin Oxygen Availability to the Upper Limb During Standardized Arm Abduction in Patients With Suspected Thoracic Outlet Syndrome
Authors: Hersant Jeanne , Lecoq Simon , Ramondou Pierre , Papon Xavier , Feuilloy Mathieu , Abraham Pierre , Henni Samir 
Published: February 2022

ObjectiveThoracic outlet syndrome (TOS) should be considered of arterial origin only if patients have clinical symptoms that are the result of documented symptomatic ischemia. Simultaneous recording of inflow impairment and forearm ischemia in patients with suspected TOS has never been reported to date. We hypothesized that ischemia would occur in cases of severely impaired inflow, resulting in a non-linear relationship between changes in pulse amplitude (PA) and the estimation of ischemia during provocative attitudinal upper limb positioning.DesignProspective single center interventional study.MaterialFifty-five patients with suspected thoracic outlet syndrome.MethodsWe measured the minimal decrease from rest of transcutaneous oximetry pressure (DROPm) as an estimation of oxygen deficit and arterial pulse photo-plethysmography to measure pulse amplitude changes from rest (PA-change) on both arms during the candlestick phase of a “Ca + Pra” maneuver. “Ca + Pra” is a modified Roos test allowing the estimation of maximal PA-change during the “Pra” phase. We compared the DROPm values between deciles of PA-changes with ANOVA. We then analyzed the relationship between mean PA-change and mean DROPm of each decile with linear and second-degree polynomial (non-linear) models. Results are reported as median [25/75 centiles]. Statistical significance was p &lt; 0.05.ResultsDROPm values ranged −11.5 [−22.9/−7.2] and − 12.3 [−23.3/−7.4] mmHg and PA-change ranged 36.4 [4.6/63.8]% and 38.4 [−2.0/62.1]% in the right and left forearms, respectively. The coefficient of determination between median DROPm and median PA-change was r2 = 0.922 with a second-degree polynomial fitting, but only r2 = 0.847 with a linear approach.ConclusionOxygen availability was decreased in cases of severe but not moderate attitudinal inflow impairments. Undertaking simultaneous A-PPG and forearm oximetry during the “Ca + Pra” maneuver is an interesting approach for providing objective proof of ischemia in patients with symptoms of TOS suspected of arterial origin.
The Psychological Impact of the Tertiary Hospital Reappraisal on Resident Doctors in the Post-pandemic Era: A Cross-sectional Study in Ningbo
Authors: Shao Zhonghao , Cao Angyang , Luo Wenjun , Zhou Yanling , Wang Jianhua , Gui Yu , Gao Bin , Xu Zhipeng , Zhu Binbin , Sheng Zhiren 
Published: February 2022

ParticipantsCompetent resident doctor were expected to help the patients, advance medical knowledge, and promote public health. The time and effort necessary for residents to devote to standarized training is extensive. Anxiety and depression can negatively affect professional development and work efficacy. The study aimed to assess the psychosocial effects of the hospital reappraisal during the post-pandemic era of COVID-19 and analyze potential risk factors leading to their symptoms of anxiety and depression.MethodIn March 2021, the “Questionnaire Star” electronic questionnaire system was used to collect data. A total of 96 resident doctors from the affiliated hospital of the medical school of Ningbo University were invited to complete the questionnaires.ResultsAccording to our study, the prevalence of symptoms of anxiety and depression in the resident doctors in the institution was 61.5 and 59.4%, respectively. The residents who were worried about clinical skills tend to have anxiety symptoms under online education (OR = 3.436, 95%CI: 1.122–10.526). Compared with participants who were assigned by other hospitals, social trainees (OR: 7.579, 95%CI: 1.747–32.885), and full-time masters (OR: 5.448, 95% CI: 1.586–18.722) were more likely to have anxiety symptoms. Participants without a labor contract (OR = 3.257, 95% CI: 1.052–10.101) had a high risk of depression symptoms. Participants who spent more time learning the details prepared for the tertiary hospital reappraisal were significantly more likely to develop anxiety and depressive symptoms.ConclusionThis study suggested that the tertiary hospital reappraisal program has an impact on the high incidence of anxiety and depression of the young resident doctors during the post-pandemic era of the COVID-19 in Ningbo.
Differences in Older Patients' Attitudes Toward Deprescribing at Contextual and Individual Level
Authors: Oktora Monika Pury , Edwina Angela Elma , Denig Petra 
Published: February 2022

BackgroundDeprescribing requires patients' involvement and taking patients' attitudes toward deprescribing into account. To understand the observed variation in these attitudes, the influence of contextual-level factors, such as country or healthcare setting, should be taken into account.MethodsWe conducted a systematic review of studies using the revised Patients' Attitudes Towards Deprescribing (rPATD) questionnaire among older adults. We searched articles in Medline and Embase up to 30 June 2021. PRISMA guideline was used for the search process and reporting. We summarized the outcomes from the rPATD and compared attitudes at study population level between high or low-middle-income countries, global regions, and healthcare settings using ANOVA testing. Correlations of the rPATD outcomes with the mean age of the study populations were tested. Associations with the rPATD outcomes at individual patient level extracted from the included studies were summarized.ResultsSixteen articles were included. Percentages of patients willing to stop medication were significantly lower in low-middle-income countries (&lt;70% in Nepal and Malaysia) compared to high-income countries (&gt;85% in USA, Australia, European countries). No significant differences were observed when results were compared by global region or by healthcare setting but a high willingness (&gt;95%) was seen in the two studies conducted in an inpatient population. A higher mean age at study level was associated with a higher willingness to stop medication. At individual level, associations between patient characteristics, including demographics and education, and attitudes toward deprescribing showed inconsistent results.ConclusionFindings about attitudes toward deprescribing are influenced by contextual factors. Future research should pay more attention to the influence of the healthcare system and setting as well as the culture on patients' attitudes.
PancreaSys: An Automated Cloud-Based Pancreatic Cancer Grading System
Authors: Sehmi Muhammad Nurmahir Mohamad , Fauzi Mohammad Faizal Ahmad , Ahmad Wan Siti Halimatul Munirah Wan , Chan Elaine Wan Ling 
Published: February 2022

Pancreatic cancer is one of the deadliest diseases which has taken millions of lives over the past 20 years. Due to challenges in grading pancreatic cancer, this study presents an automated cloud-based system, utilizing a convolutional neural network deep learning (DL) approach to classifying four classes of pancreatic cancer grade from pathology image into Normal, Grade I, Grade II, and Grade III. This cloud-based system, named PancreaSys, takes an input of high power field images from the web user interface, slices them into smaller patches, makes predictions, and stitches back the patches before returning the final result to the pathologist. Anvil and Google Colab are used as the backbone of the system to build a web user interface for deploying the DL model in the classification of the cancer grade. This work employs the transfer learning approach on a pre-trained DenseNet201 model with data augmentation to alleviate the small dataset’s challenges. A 5-fold cross-validation (CV) was employed to ensure all samples in a dataset were used to evaluate and mitigate selection bias during splitting the dataset into 80% training and 20% validation sets. The experiments were done on three different datasets (May Grunwald-Giemsa (MGG), hematoxylin and eosin (H&amp;E), and a mixture of both, called the Mixed dataset) to observe the model performance on two different pathology stains (MGG and H&amp;E). Promising performances are reported in predicting the pancreatic cancer grade from pathology images, with a mean f1-score of 0.88, 0.96, and 0.89 for the MGG, H&amp;E, and Mixed datasets, respectively. The outcome from this research is expected to serve as a prognosis system for the pathologist in providing accurate grading for pancreatic cancer in pathological images.
Predicting Cervical Lymph Node Metastasis Following Endoscopic Surgery in Superficial Head and Neck Carcinoma
Authors: Okabe Ryuichi , Ueki Yushi , Ohashi Riuko , Takeuchi Manabu , Hashimoto Satoru , Takahashi Takeshi , Shodo Ryusuke , Yamazaki Keisuke , Matsuyama Hiroshi , Umezu Hajime , Terai Shuji , Ajioka Yoichi , Horii Arata 
Published: February 2022

BackgroundEarly detection of head and neck carcinoma (HNC) as superficial HNC (SHNC) identified using recently developed optical techniques, such as magnifying endoscopy and narrow-band imaging (NBI), in combination with endoscopic surgeries enables minimally invasive treatment with favorable outcomes for HNC. This study aimed to identify the predictive factors for the rare but important clinical issue of SHNC, namely cervical lymph node metastasis (CLNM), following endoscopic resection.MethodsSixty-nine patients with SHNC who underwent endoscopic resection were enrolled in the study. Clinical data, preoperative endoscopic findings, pathological findings, and treatment outcomes were retrospectively reviewed. Because the pharyngeal mucosa lacks the muscularis mucosa, we measured tumor thickness in permanent pathology as an alternative to the depth of invasion. Correlations with the occurrence of CLNM were statistically examined.ResultsThe 5-year disease-specific survival rate was 100%. Of 69 patients, 3 (4.3%) developed CLNM. All had subepithelial but not epithelial tumors. The 0-IIa type in the macroscopic findings, type B2/B3 vessels in narrow-band imaging, tumors ≥ pathological stage T2, lymphatic invasion, positive surgical margins, and tumor thickness &gt;1,000 μm showed significant correlations with CLNM following endoscopic resection. Furthermore, the classification of type B vessels was significantly associated with tumor thickness.ConclusionThe treatment outcomes following endoscopic resection for SHNC were favorable. The risk of CLNM following endoscopic resection in SHNC can be predicted by several preoperative endoscopic and postoperative pathological findings. Among them, the classification of type B vessels, which correlated with both tumor thickness and CLNM, might be a useful predictive factor.
PANORAMA DE LA ENSEÑANZA Y APRENDIZAJES DE LA EVALUACIÓN DE LA POLÍTICA SOCIAL EN MÉXICO (2012-2018)
Authors: Lara-Caballero Manuel , Martínez-Mendoza Abigail , Figueroa-Romero Raúl 
Published: February 2022

La investigación analizó las evaluaciones externas de la política social en México, durante el periodo 2012-2018, con el objetivo de identificar el aprendizaje instrumental, social o táctico. El análisis consistió en revisar el Programa Anual de Evaluación (PAE), los informes finales de las evaluaciones, y los aspectos susceptibles de mejora de cada evaluación, considerando únicamente las evaluaciones con información completa en sus informes. Los resultados muestran un decremento en el total de evaluaciones en dicho periodo; se observa discrepancia entre la información del PAE y los programas presupuestarios; fueron realizadas 146 evaluaciones de diseño, ninguna de indicadores, 5 de impacto, siendo estas las más costosas; de los actores responsables de realizar las evaluaciones externas de diseño, destacan las instituciones de educación superior. El aprendizaje instrumental es el que más se genera porque todas las metodologías están enfocadas hacia la viabilidad y los medios de acción para lograr la eficacia. La principal conclusión es que las metodologías de evaluación propuestas por el CONEVAL tienen relación con las fases de las políticas públicas porque representan procesos incrementales de aprendizaje que deben estar sustentados en ejercicios sistemáticos de evaluación, pero no logran el ciclo completo de las evaluaciones.
MENINGKATKAN PENGUASAAN VOCABULARIES SISWA MELALUI LAGU – LAGU BAHASA INGGRIS DI KELAS VIIl-2 SMP NEGERI 2 MARDINGDING SEMESTER GANJIL TAHUN PELAJARAN 2015/2016
Authors: Br Sinulingga Jendakita 
Published: February 2022

ABSTRACTThis study aims to improve student learning outcomes in learning English by using themedia of English songs. The subjects of this study were 27 students of class VIII-2 ofSMP Negeri 2 Mardingding, Mardingding District, Karo Regency, consisting of 11 malestudents and 16 female students. The research method applied is a classroom actionresearch method. Through two learning cycles, each cycle is carried out with planning,implementation, observation and reflection activities. Based on the results of dataanalysis, it is known that by using the media of English songs in English lessons in classVIII-2 of SMP Negeri 2 Mardingding, Mardingding District, Karo Regency, 2015/2016Academic Year, mastery of students' vocabularies has increased from Cycle First toCycle Second. Mastery of students' vocabularies in the first cycle obtained an averagescore of 30 with the percentage of the acquisition score of the total score of 30% and thisis in the very poor category (not good). And far from KKM (70). In the second cycle ofvocabulary mastery students obtained an average score of 80 with a percentage of theacquisition score of a total score of 80% and this is in a good category and hasexceeded KKM (70). So, the increase in students' vocabularies mastery from cycle I tocycle II was 50%, while student learning outcomes after using English songs as media inlearning English also increased. Likewise, the number of students who have completedthe KKM has reached the indicators of success, where the standard is 80%, while theacquisition is 96%.
Keywords: Using English songs improves mastery of English vocabularies
Students’ Speaking Skill Trough Storytelling Technique At Smp Negeri 4 Tanjung Morawa
Authors: Hutabarat Marta Karina , Limbong Yohana Dona Pika , D Madina 
Published: February 2022

Abstract

A speaking talent is the ability to say or recite an idea that a person has, and it includes features of pronunciation, vocabulary, and syntax. The objective of speaking is to express a thought or sensation that has arisen in the individual as a result of listening to music or learningcontent. The storytelling technique is one way to help pupils enhance their speaking abilities. Storytelling is a fascinating practice that encourages children to use their imaginations.Storytelling can assist them improve their language abilities while also deepening and broadening their comprehension of the story's characters.
A qualitative research design is used in this study. The goal of this study is to help researchers and readers understand what media and approaches teachers use in the classroom to help students, particularly junior high school students, enhance their vocabulary.

Keywords : Storytelling, Effect, Speaking skill
REVITALISASI LEGENDA BATU HODA MELALUI NASKAH DRAMA
Authors: Donganta Donganta , Harapan Febri Yanti Arfansyah , Sari Sartika 
Published: February 2022

ABSTRACT
The legend of Batu Hoda is a folk tale from the Simanindo sub-district, Samosir district, which tells of a mare who is waiting for her lover a stallion to meet on the shores of Lake Toba. The form of the story about the origin of Batu Hoda is not yet known by many people, this is due to the lack of innovation in telling stories to the community, especially children. Therefore, people have lost interest in telling or curiosity about legends. The research method used is a qualitative approach with the aim of finding information about the story of Batu Hoda. As the main design, such as interviews with several informants or the surrounding community. The results of this study were formulated in the form of re-searching the legend of Batu Hoda in the form of presenting the story that was played. The selection of drama script media as media is expected to be one of the efforts to preserve folklore. The drama script provides a retelling style with dasi and illustrations and makes it easier for people to get carried away in the Batu Hoda story.
Keywords: Simanindo's glasses, Folklore, Drama Script, Batu Hoda