bluuebunny/crossref_metadata_2025_split huggingface dataset:
Olive polyphenols and bioavailable glutathione: Promising results in patients diagnosed with mild Alzheimer's disease
Authors: Marianetti Massimo , Pinna Silvia , Venuti Angelo , Liguri Gianfranco 
Published: March 2022

AbstractIntroductionRecent studies highlighted the role of olive polyphenols in disrupting the ordered structure of highly cytotoxic amyloid beta protofibrils and the efficacy of a derivatized form of glutathione to counteract neuronal oxidative stress affecting specific brain regions at early stages of Alzheimer's disease (AD) pathogenesis. We performed a randomized cross‐over clinical trial to evaluate their potential benefits in mild AD.MethodsOleuropein and S‐acetyl glutathione were administered as dietary supplement for 6 months to 18 patients diagnosed for probable mild AD according to International Working Group 2 criteria. Patients underwent an extensive cognitive and behavioral neuropsychological test battery at the beginning and end of the study to evaluate cognitive deterioration, memory, visuospatial abilities, attention, language, executive functions, and behavioral disorders. We compared patients receiving treatment to patients receiving no treatment.ResultsAll the measured neurocognitive parameters stabilized or improved after the treatment in all patients.DiscussionDietary supplement with olive polyphenols and bioavailable glutathione could be useful for patients diagnosed with mild AD.
Cover Image, Volume 9, Issue 2
Authors: Huang Zeqing , Zhao Tongtiegang 
Published: March 2022

The cover image is based on the Advanced Review Predictive performance of ensemble hydroclimatic forecasts: Verifi cation metrics, diagnostic plots and forecast attributes by Zeqing Huang and Tongtiegang Zhao https://doi.org/10.1002/wat2.1580.image
The averaged null energy condition on holographic evaporating black holes
Authors: Ishibashi Akihiro , Maeda Kengo 
Published: March 2022

Abstract
We examine the averaged null energy condition (ANEC) for strongly coupled fields, along the event horizon of an evaporating black hole by using the AdS/CFT duality. First, we consider a holographic model of a 3-dimensional evaporating black hole with a perturbed 4-dimensional black droplet geometry as the bulk dual, and investigate how negative energy flux going into the boundary black hole horizon appears. We show that the ingoing negative energy flux always appears at the boundary black hole horizon when the horizon area decreases. Second, we test the ANEC in a holographic model whose boundary geometry is a 4-dimensional asymptotically flat spacetime, describing the formation and subsequent evaporation of a spherically symmetric black hole. By applying the “bulk-no-shortcut principle”, we show that the ANEC is always satisfied when the local null energy is averaged with a weight function along the incomplete null geodesic on the event horizon from beginning of the formation to the final instant of the black hole evaporation. Our results indicate that the total ingoing negative energy flux is compensated by a large amount of positive energy flux in the early stage of the black hole formation.
N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion
Authors: Hobohm Laura , Koudelka Tomas , Bahr Fenja H. , Truberg Jule , Kapell Sebastian , Schacht Sarah-Sophie , Meisinger Daniel , Mengel Marion , Jochimsen Alexander , Hofmann Anna , Heintz Lukas , Tholey Andreas , Voss Matthias 
Published: March 2022

AbstractGolgi membrane proteins such as glycosyltransferases and other glycan-modifying enzymes are key to glycosylation of proteins and lipids. Secretion of soluble Golgi enzymes that are released from their membrane anchor by endoprotease activity is a wide-spread yet largely unexplored phenomenon. The intramembrane protease SPPL3 can specifically cleave select Golgi enzymes, enabling their secretion and concomitantly altering global cellular glycosylation, yet the entire range of Golgi enzymes cleaved by SPPL3 under physiological conditions remains to be defined. Here, we established isogenic SPPL3-deficient HEK293 and HeLa cell lines and applied N-terminomics to identify substrates cleaved by SPPL3 and released into cell culture supernatants. With high confidence, our study identifies more than 20 substrates of SPPL3, including entirely novel substrates. Notably, our N-terminome analyses provide a comprehensive list of SPPL3 cleavage sites demonstrating that SPPL3-mediated shedding of Golgi enzymes occurs through intramembrane proteolysis. Through the use of chimeric glycosyltransferase constructs we show that transmembrane domains can determine cleavage by SPPL3. Using our cleavage site data, we surveyed public proteome data and found that SPPL3 cleavage products are present in human blood. We also generated HEK293 knock-in cells expressing the active site mutant D271A from the endogenous SPPL3 locus. Immunoblot analyses revealed that secretion of select novel substrates such as the key mucin-type O-glycosylation enzyme GALNT2 is dependent on endogenous SPPL3 protease activity. In sum, our study expands the spectrum of known physiological substrates of SPPL3 corroborating its significant role in Golgi enzyme turnover and secretion as well as in the regulation of global glycosylation pathways.
Matching in Power Graphs of Finite Groups
Authors: Cameron Peter J. , Swathi V. V. , Sunitha M. S. 
Published: March 2022

AbstractThe power graph P(G) of a finite group G is the undirected simple graph with vertex set G, where two elements are adjacent if one is a power of the other. In this paper, the matching numbers of power graphs of finite groups are investigated. We give upper and lower bounds, and conditions for the power graph of a group to possess a perfect matching. We give a formula for the matching number for any finite nilpotent group. In addition, using some elementary number theory, we show that the matching number of the enhanced power graph $$P_e(G)$$

P
e

(
G
)

of G (in which two elements are adjacent if both are powers of a common element) is equal to that of the power graph of G.
Comparative effectiveness of moderate hypofractionation with volumetric modulated arc therapy versus conventional 3D-radiotherapy after radical prostatectomy
Authors: Moll Matthias , D’Andrea David , Zaharie Alexandru , Grubmüller Bernhard , Paschen Christopher , Zehetmayer Sonja , Shariat Shahrokh F. , Widder Joachim , Goldner Gregor 
Published: March 2022

Abstract
Purpose
Hypofractionated radiotherapy for prostate cancer is well established for definitive treatment, but not well defined in the postoperative setting. The purpose of this analysis was to assess oncologic outcomes and toxicity in a large cohort of patients treated with conventionally fractionated three-dimensional (3D) conformal radiotherapy (CF) and hypofractionated volumetric modulated arc therapy (HF) after radical prostatectomy.

Methods
Between 1994 and 2019, a total of 855 patients with prostate carcinoma were treated by postoperative radiotherapy using CF (total dose 65–72 Gy, single fraction 1.8–2 Gy) in 572 patients and HF (total dose 62.5–63.75 Gy, single fraction 2.5–2.55 Gy) in 283 patients. The association of treatment modality with biochemical control, overall survival (OS), and gastrointestinal (GI) and genitourinary (GU) toxicity was assessed using logistic and Cox regression analysis.

Results
There was no difference between the two modalities regarding biochemical control rates (77% versus 81%, respectively, for HF and CF at 24 months and 58% and 64% at 60 months; p = 0.20). OS estimates after 5 years: 95% versus 93% (p = 0.72). Patients undergoing HF had less frequent grade 2 or higher acute GI or GU side effects (p = 0.03 and p = 0.005, respectively). There were no differences in late GI side effects between modalities (hazard ratio 0.99). Median follow-up was 23 months for HF and 72 months for CF (p < 0.001).

Conclusion
For radiation therapy of resected prostate cancer, our analysis of this largest single-centre cohort (n = 283) treated with hypofractionation with advanced treatment techniques compared with conventional fractionation did not yield different outcomes in terms of biochemical control and toxicities. Prospective investigating of HF is merited.
Analysis of poplar timber finger joints by means of Digital Image Correlation (DIC) and finite element simulation subjected to tension loading
Authors: Timbolmas Cristian , Rescalvo Francisco J. , Portela María , Bravo Rafael 
Published: March 2022

AbstractDue to the strong demand for wood, and the need to reduce the carbon footprint in construction, fast-growing low-graded planted species like poplar are promising wood for the supply chain in the context of engineered wood products, EWPs. Finger joints constitute a key technology for the manufacture of long structural elements of EWPs. Thus, evaluation of the mechanical properties of finger joints is important when designing these structural members. This paper shows the results of mechanical behaviour in tension of poplar timber of the I-214 cultivar, for specimens with and without finger joints, using experiments monitored by DIC (Digital Image Correlation) and simulated by the Finite Element Method. For moderate and intermediate loads, the samples with finger joints showed behaviour similar to those without joints. However, the presence of the fingers decreased the mechanical properties of the modulus of elasticity in the longitudinal direction anywhere from 7.7 to 23.7%; and there was a decrease of around 27.5% in tensile strength. An agreeable correlation between the DIC results and FEM simulations is obtained for the longitudinal, transversal, and shear strain fields, thus demonstrating the high potential of both methodologies.
Technological mediation and 3D visualizations in construction engineering practice
Authors: Voordijk Hans , olde Scholtenhuis Léon 
Published: March 2022

AbstractThe generation and use of 3D images and visualizations through remote sensing, Building Information Modeling, and Augmented Reality technologies, have come to play a significant role in construction engineering practice. Although these technologies are promising, their potential can be misjudged when potential end-users are unaware of key assumptions that were made by developers. Realistic expectations require insights into the ways in which these technologies transform input collected into 3D visualizations and how these visualizations are possibly used on construction sites. This study’s objective is hence to explore the form of technological mediation that the generation and use of 3D images and visualizations provide between a human and objects, or aspects of these objects, that would otherwise be largely imperceptible to professionals in construction practice. We show that algorithms pre- and post-process data through their technological selectivities, which function as mediators. Double mediations of augmented and engaged relationships play a dominant role in the use of 3D images and visualizations and enhance the situational awareness of professionals in construction practice. This is the first study that applies this perspective to increase the understanding of the mediating role of 3D images and visualizations in construction practice.
Classification of rank two weak Fano bundles on del Pezzo threefolds of degree four
Authors: Fukuoka Takeru , Hara Wahei , Ishikawa Daizo 
Published: March 2022

AbstractWe classify rank two vector bundles on a given del Pezzo threefold of degree four whose projectivizations are weak Fano into seven cases. We also give an example for each of these seven cases.
Hybrid argon plasma coagulation (HybridAPC) versus sharp excision for the treatment of endometriosis: a prospective randomized clinical trial
Authors: Keckstein Julia S. , Keckstein Simon , Brunecker Kristin , Neugebauer Alexander , Nüssle Daniela , Hoffmann Sascha , Andress Jürgen , Neis Felix , Scharpf Marcus , Enderle Markus , Rothmund Ralf , Brucker Sara Y. , Jun Martin Weiss , Kraemer Bernhard 
Published: March 2022

Abstract
Purpose
Endometriosis is a benign, but potentially serious gynaecological condition in terms of abdominal pain and impaired fertility. Laparoscopic excision techniques are considered the therapeutic standard. HybridAPC is presented as a novel technique for the non-contact thermal ablation of peritoneal endometriosis with simultaneous protection of the underlying thermosensitive structures by creating a needle-free elevated fluid cushion which enables a safer exposure and distance, as well as potentially improved peritoneal conditioning prior to APC.

Methods
In this prospective randomized clinical trial, 39 patients with 132 superficial endometriotic lesions in total were treated with HybridAPC or sharp excision in an initial laparoscopic procedure according to randomization. In a second-look laparoscopy, adhesion formation was rated macroscopically. Histologic samples were taken from previously treated areas for evaluation of eradication rate.

Results
The eradication rate was not significantly different between HybridAPC treatment and sharp excision (65 vs. 81%, p = .55). Adhesions formed in 5% of HybridAPC-treated lesions and in 10% after sharp excision (p = .49). HybridAPC treatment was significantly faster than sharp excision (69 vs. 106 s, p < .05). No intra- and postoperative complications were registered.

Conclusion
This clinical trial demonstrates the feasibility of this novel surgical technique with a promising impact on adhesion prevention. Compared to sharp excision, HybridAPC is likely to be a safe, tissue-preserving, and fast method for the treatment of peritoneal endometriosis.
Antenatal screening for chromosomal abnormalities
Authors: Kagan Karl Oliver , Sonek Jiri , Kozlowski Peter 
Published: March 2022

AbstractScreening for chromosomal disorders, especially for trisomy 21, has undergone a number of changes in the last 50 years. Today, cell-free DNA analysis (cfDNA) is the gold standard in screening for trisomy 21. Despite the advantages that cfDNA offers in screening for common trisomies, it must be recognized that it does not address many other chromosomal disorders and any of the structural fetal anomalies. In the first trimester, the optimal approach is to combine an ultrasound assessment of the fetus, which includes an NT measurement, with cfDNA testing. If fetal structural defects are detected or if the NT thickness is increased, an amniocentesis or a CVS with at least chromosomal microarray should be offered.
Potential of blood-based biomarker approaches in endometrium and breast cancer: a case-control comparison study
Authors: Schuhn Anne , Tobar Tania Witte , Gahlawat Aoife Ward , Hauke Jana , Baumann Lukas , Okun Jürgen G. , Nees Juliane 
Published: March 2022

Abstract
Purpose
Endometrial carcinoma is the second most common gynecological malignancy. Until today lacking a screening tool. A blood-based biomarker could help address this need.

Methods
The expression levels of 30 acylcarnitines, 18 amino acids, 6 miRNAs, and 7 DNA methylation sites were measured in blood samples from 331 women (20 EC, 14 benign uterine lesions (benign), 140 breast cancers (BC), 157 controls). Areas under the ROC curves (AUC), sensitivity (sens.) and specificity (spec.) were computed to identify the variables best distinguishing.

Results
The best top ten markers for the four comparisons (cancer vs. cancer-free; EC vs. BC, EC vs. controls; EC vs. benign), were identified via AUC. Malonylcarnitine distinguished best patients with EC from controls (AUC: 0.827, sens. 80%, spec. 73.1%) or BC (AUC: 0.819, sens. 84.3%, spec. 80%) being most notable. Tryptophan best differentiated benign from EC (AUC: 0.846, sens. 70%, spec. 92.9%).

Conclusions
The levels of the analyzed blood markers yielded promising results in the detection of EC and warrant further evaluation.
The plastid-nucleus localized DNA-binding protein WHIRLY1 is required for acclimation of barley leaves to high light
Authors: Saeid Nia Monireh , Repnik Urska , Krupinska Karin , Bilger Wolfgang 
Published: March 2022

Abstract
Main conclusion
In accordance with a key role of WHIRLY1 in light-acclimation mechanisms, typical features of acclimation to high light, including photosynthesis and leaf morphology, are compromised in WHIRLY1 deficient plants.

Abstract
Acclimation to the environment requires efficient communication between chloroplasts and the nucleus. Previous studies indicated that the plastid-nucleus located WHIRLY1 protein is required for the communication between plastids and the nucleus in situations of high light exposure. To investigate the consequences of WHIRLY1 deficiency on the light acclimation of photosynthesis and leaf anatomy, transgenic barley plants with an RNAi-mediated knockdown of HvWHIRLY1 were compared to wild-type plants when growing at low and high irradiance. While wild-type plants showed the typical light acclimation responses, i.e. higher photosynthetic capacity and thicker leaves, the WHIRLY1 deficient plants were not able to respond to differences in irradiance. The results revealed a systemic role of WHIRLY1 in light acclimation by coordinating responses at the level of the chloroplast and the level of leaf morphology.
Effect of pressure ratio on the separation performances of different DRPSA configurations under equilibrium conditions
Authors: Rossi Ester , Florit Federico , Storti Giuseppe , Rota Renato 
Published: March 2022

AbstractDual reflux pressure swing adsorption is a peculiar application of pressure swing adsorption with relevant separation potential. In a proper range of operating parameters, high separation performances are achieved in many applications, including complete separation in the case of binary mixtures. In this work, a new representation of the design parameters suitable for complete separation based on the semi-analytical solution of the corresponding Equilibrium Theory model is proposed for the four basic process configurations. Namely, given feed position, feed composition, and adsorbent separation selectivity, the combinations of the remaining process parameters ensuring complete separation are identified in a 3D plot. Furthermore, the same model equations have been solved numerically to explore conditions of incomplete separation, where the semi-analytical solution is not available. In particular, the sensitivity of the separation performances of each configuration to the pressure ratio has been explored. More specifically, given the region of operating conditions suitable for complete separation, selected operating conditions outside this region have been explored aimed to recover/improve the separation quality. Even though the different configurations exhibit different behaviors, the general dependence of the product purity upon the pressure ratio is quite limited and only minor improvements can be obtained with a few exceptions.
Sex Selection for Daughters: Demographic Consequences of Female-Biased Sex Ratios
Authors: Kolk Martin , Jebari Karim 
Published: March 2022

AbstractModern fertility techniques allow parents to carry out preimplantation sex selection. Sex selection for non-medical purposes is legal in many high-income countries, and social norms toward assisted reproductive technology are increasingly permissive and may plausibly become increasingly prevalent in the near future. We explore possible outcomes of widely observed daughter preferences in many high-income countries and explore the demographic consequences of the adoption of sex selection for daughters. While concerns over son preference have been widely discussed, sex selection that favors female children is a more likely outcome in high-income countries. If sex selection is adopted, it may bias the sex ratio in a given population. Male-biased populations are likely to experience slower population growth, which limits the long-term viability of corresponding cultural norms. Conversely, female-biased populations are likely to experience faster population growth. Cultural norms that promote female-biased sex ratios are as a consequence therefore also self-reinforcing. In this study, we explore the demographic consequences of a female-biased sex ratio for population growth and population age structure. We also discuss the technology and parental preferences that may give rise to such a scenario.
Psb34 protein modulates binding of high-light-inducible proteins to CP47-containing photosystem II assembly intermediates in the cyanobacterium Synechocystis sp. PCC 6803
Authors: Rahimzadeh-Karvansara Parisa , Pascual-Aznar Guillem , Bečková Martina , Komenda Josef 
Published: March 2022

AbstractAssembly of photosystem II (PSII), a water-splitting catalyst in chloroplasts and cyanobacteria, requires numerous auxiliary proteins which promote individual steps of this sequential process and transiently associate with one or more assembly intermediate complexes. In this study, we focussed on the role of a PSII-associated protein encoded by the ssl1498 gene in the cyanobacterium Synechocystis sp. PCC 6803. The N-terminal domain of this protein, which is here called Psb34, is very similar to the N-terminus of HliA/B proteins belonging to a family of high-light-inducible proteins (Hlips). Psb34 was identified in both dimeric and monomeric PSII, as well as in a PSII monomer lacking CP43 and containing Psb28. When FLAG-tagged, the protein is co-purified with these three complexes and with the PSII auxiliary proteins Psb27 and Psb28. However, the preparation also contained the oxygen-evolving enhancers PsbO and PsbV and lacked HliA/B proteins even when isolated from high-light-treated cells. The data suggest that Psb34 competes with HliA/B for the same binding site and that it is one of the components involved in the final conversion of late PSII assembly intermediates into functional PSII complexes, possibly keeping them free of Hlips. Unlike HliA/B, Psb34 does bind to the CP47 assembly module before its incorporation into PSII. Analysis of strains lacking Psb34 indicates that Psb34 mediates the optimal equilibrium of HliA/B binding among individual PSII assembly intermediates containing CP47, allowing Hlip-mediated photoprotection at all stages of PSII assembly.
Begrenzte Möglichkeiten. Zur Kommerzialisierung und Akademisierung der Intellektuellenrolle
Authors: Martin Susanne 
Published: March 2022

ZusammenfassungZwei traditionelle Wirkungsbereiche von Intellektuellen, die politische Medienöffentlichkeit und das akademische Feld, unterliegen seit über drei Jahrzehnten anhaltenden strukturellen Veränderungen. Diese gelten vielfach als Ursache einer tiefen Krise oder sogar des Verschwindens der Intellektuellen. Doch um welche Veränderungen geht es dabei genau, und wie restrukturieren sie die gegenwärtige Rolle und Funktion von Intellektuellen? Zur Beantwortung dieser Fragen entwickelt der Beitrag einen Ansatz, der die struktur- und erfahrungsbezogenen Bedingungen intellektueller Praxis fokussiert und historisch vergleichend analysiert. Um eine Vergleichsfolie zu gewinnen, wird die intellektuelle Praxis Theodor W. Adornos analysiert. Dabei zeigt sich, dass Adorno die charakteristischen Widersprüche öffentlichen und akademischen intellektuellen Engagements methodisch aufrechterhielt, indem er eine Position des „Dazwischen“ reklamierte. Vor diesem Hintergrund werden seit den 1970er-Jahren forcierte strukturelle Veränderungen der Medienöffentlichkeit und des akademischen Feldes als Prozesse der „Vereindeutigung“ interpretiert, die eine widerspruchsaffine intellektuelle Praxis erschweren. In der Folge lassen sich eine ausgeweitete kommerzielle sowie eine eingeschränkte akademische Intellektuellenpraxis beobachten, die jeweils politisch wirksame Interventionen begrenzen.