bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of two port dual band dual circularly polarized slot antenna with diversity for wireless application
Authors: Saini Rohit Kumar , Dwari Santanu , Kumar Pawan 
Published: November 2022

Abstract
In this article, a novel two-port dual-band dual-circularly polarized slot antenna with diversity is designed for wireless application. The antenna consists of a square slot, two asymmetric T-shaped feed lines in orthogonal direction protrude from signal lines, and an arrow shaped grounded stub at the inner corner of the ground slot between the two orthogonal feed lines at an angle of 45ᵒ. Orthogonal feed lines are used for generation of circular polarized waves. If we apply the excitation at another port the sense of circular polarization is interchange in both the bands of axial ratio. The lower band of axial ratio covers frequency range of 1.42-2.25GHz with 45.23% and fc 1 =1. 85GHz.Same for upper band covers frequency range of 3.43–3.65 GHz with 6.22% and fc 2 =3.3GHz. The two ports of antenna have isolation variation from 10 to12 dB and 17 to 18dB in the lower and upper band of axial ratio. The proposed antenna has isolation between lower and upper bands of impedance bandwidth also.
Exome sequencing in individuals with congenital anomalies of the kidney and urinary tract (CAKUT): a single-center experience
Authors: Hoefele Julia , Riedhammer Korbinian , Ćomić Jasmina , Tasic Velibor , Putnik Jovana , Abazi-Emini Nora , Stajić Nataša , Meitinger Thomas , Nushi-Stavileci Valbona , Berutti Riccardo , Braunisch Matthias , Paripovic Aleksandra 
Published: November 2022

Abstract
Individuals with congenital anomalies of the kidney and urinary tract (CAKUT) show a broad spectrum of malformations. CAKUT can occur in an isolated fashion or as part of a syndromic disorder and can lead to end-stage kidney failure. A monogenic cause can be identified in approximately 12% of affected individuals. This study investigated a single-center CAKUT cohort analyzed by exome sequencing (ES). Emphasis was placed on the question whether diagnostic yield differs between certain CAKUT phenotypes (e.g., bilateral kidney affection, unilateral kidney affection or only urinary tract affection). 86 unrelated individuals with CAKUT were categorized according to their phenotype and analyzed by ES to identify a monogenic cause. Prioritized variants were rated according to recommendations of the American College of Medical Genetics and Genomics and the Association for Clinical Genomic Science. Diagnostic yields of different phenotypic categories were compared. Clinical data were collected using a standardized questionnaire. In the study cohort, 7/86 individuals had a (likely) pathogenic variant in the genes PAX2, PBX1, EYA1 or SALL1. Additionally, in one individual, a 17q12 deletion syndrome (including HNF1B) was detected. 62 individuals had a kidney affection, which was bilateral in 36. All solved cases (8/86, 9%) had bilateral kidney affection (diagnostic yield in subcohort: 8/36, 22%). Although the diagnostic yield in CAKUT cohorts is low, our single-center experience argues, that, in individuals with bilateral kidney affection, monogenic burden is higher than in those with unilateral kidney or only urinary tract affection.
Association between elevated plasma glucose levels and medication-related osteonecrosis of the jaw
Authors: Kammerhofer Gabor , Vegh Daniel , Joob-Fancsaly Arpad , Geczi Zoltan , Somogyi Kata Sara , Biczo Zita , Juhasz Donat Huba , Ujpal Marta , Vaszilko Mihaly Tamas , Nemeth Zsolt 
Published: November 2022

Abstract
Objectives: Medication-related osteonecrosis of the jaw (MRONJ) is a type of jawbone necrosis caused by the use of drugs for some types of cancer and osteoporosis. Previous research has shown that normoglycemia plays an important rolein the development and treatment of oral lesions. The current study aimed to evaluate the associationsbetween chronic vascular disease, inflammation, and immune deficiency during dysglycemia and the developmentof MRONJ. Materials and Methods: Our research group investigated data collected between January 1, 2019, and December 31, 2020. In total, 260 patients at the Inpatient Care Unit, Department of Oromaxillofacial Surgery and Stomatology, Semmelweis University were selected.
Results: There was a significant association between hyperglycemia and MRONJ. Vascular anomaly and immune dysfunction caused byhyperglycemia can leadto the development of necrosis after tooth extraction. The risk of jawbone necrosis can be reduced by premedication.
Conclusions: Ischemia is a complication of abnormal glucose level, which is a possible risk factor of MRONJ development. Hence, uncontrolled or poorly regulated plasma glucose levels can significantly increase the risk of jawbone necrosis.
Clinical relevance: Uncontrolled or poorly regulated plasma glucose levels significantly increasesthe risk of jawbone necrosis after invasive dental or oral surgical interventions. The number of patients with dysglycemia and anti-resorptive therapy is increasing. Therefore, patients with these conditions will be provided with medical care in dental offices more frequently.
The association between the problem list and chronic pain management
Authors: Rylee Tina L. , Copenhaver David , Drake Christiana , Joseph Jill 
Published: November 2022

Abstract
Background
Roughly a quarter of the US population suffers from moderate to severe chronic pain for at least six months in any given year. The complexity of managing chronic pain has encouraged providers to use innovative methods to address it. Research has shown that problem lists are potential tools that support the care of patients with diabetes and chronic kidney disease.
Objectives
To examine the extent to which the inclusion of chronic pain in a problem list is associated with follow-up specialty pain care.
Methods
The association between chronic pain documentation on the problem list and specialty pain care was investigated in this retrospective cohort study using 4,531 patient records.
Results
Chronic pain documentation in the problem list was associated with higher odds of receiving specialty pain care. The most common diagnosis was chronic pain (69.7%). A migraine diagnosis was associated with decreased odds of receiving specialty care, and chronic pain syndrome was associated with increased odds of receiving specialty care compared with the other chronic pain groups.
Conclusion
Documenting chronic pain on the problem list was associated with a higher likelihood of patients receiving specialty pain care.
Laser-induced antibacterial coating on the surface of an individual titanium membrane designed by a neural network
Authors: Karlagina Yulia , Shelemanov Andrey , Zilberman Vladimir , Zernitckaia Ekaterina , Lovushkina Elizaveta , Zavialova Anastasia , Rogachev Kirill , Afanasiev Nikita , Kolobov Yuri , Manokhin Sergey , Evstropiev Sergey , Odintsova Galina 
Published: November 2022

Abstract
In modern dentistry dental implants are widely used to replace lost teeth. However, the placement of an implant may not be possible due to bone atrophy. One of the most modern ways to restore jawbone after atrophy is the use of barrier membranes. This paper proposes a method to design and create an improved barrier membranes using neural networks and laser technology. This development allows to obtain a personalized titanium membrane with a laser-induced antibacterial coating. At the first step the membrane is designed based on CT scan of the patient using 3D modeling software and convolutional neural network U-net, which allows us to reduce modeling time. The second step is to create membrane by micromilling from a titanium block. Finally, at the third step we create antibacterial membrane surface under laser oxidation and UV-activation of singlet oxygen production on the surface.
The performance of the MODY calculator in a non-Caucasian, mixed-race population diagnosed with diabetes mellitus before age 35 years
Authors: Junior Augusto Cezar Santomauro , Magalhães Áurea Luiza Fernandes , Motta Flávia Tedesco , Santana Lucas Santos , Franco Pedro Campos , Freitas Silvia Maria , Sanchez Jeniffer Johana Duarte , Costa-Riquetto Aline Dantas , Teles Milena G. 
Published: November 2022

Abstract
Backgroud: A maturity-onset diabetes of the young (MODY) calculator has been described and validated for use in European Caucasians. This study evaluated its performance in Brazilians diagnosed with diabetes mellitus (DM) before 35 years of age.
Methods: The electronic records of 391 individuals monitored between 1953 and 2020 at the diabetes clinic of a quaternary hospital in São Paulo were analyzed: 231 with type 1 DM (T1DM), 46 with type 2 (T2DM) and 114 with MODY. The MODY calculator was applied to the three groups. A receiver operating characteristic curve was calculated to obtain cut-off points for this population.
Results: The principal differences between the MODY and the T1DM and T2DM groups were body mass index, a positive family history of diabetes and mean HbA1c level. Age at diagnosis in the MODY group was only significantly different compared to the T2DM group. Specificity and sensitivity were good for the cut-off points of 40%, 50% and 60%, with the accuracy of the model for any of these cut-off points being >95%.
Conclusion: The capacity of the calculator to identify Brazilian patients with MODY was good. Values ≥60% proved useful for selecting candidates for MODY genetic testing, with good sensitivity and specificity.
Evaluation of a new robotic system for infant surgery – a study in piglets of less than 10kg body weight.
Authors: Krebs Thomas F. , Kayser Timo , Lorenzen Ulf , Grünewald Matthias , Kayser Marit , Saltner Anna , Durmaz Lidya-Olgu , Reese Lina Johanna , Brownlee Ewan , Reischig Katja , Baastrup Jonas , Meinzer Andreas , Kalz Almut , Becker Thomas , Bergholz Robert 
Published: November 2022

Abstract
BACKGROUND:

We were able to demonstrate the feasibility of a new robotic system (Versius, CMR Surgical, Cambridge, England) for procedures in small inanimate cavities. The aim of this consecutive study was to test the Versius® system for its feasibility, performance and safety of robotic abdominal and thoracic surgery in piglets simulating infants with a body weight lower than 10kg.

METHODS:

24 Procedures (from explorative laparoscopy to thoracoscopic esophageal repair) were performed in 4 piglets with a mean age of 12 days days and a mean body weight of 6.4 (7–7.5) kg. Additional urological procedures were performed after euthanasia of the piglet. The Versius® robotic system was used with 5mm wristed instruments and a 10 mm 3D 0° or 30° camera. The setup consisted of the master console and three to four separate arms. The performance of the procedure, the size, position and the distance between the ports, the external and internal collisions and complications of the procedures were recorded and analyzed.

RESULTS:

We were able to perform all surgical procedures as planned. We encountered neither surgical nor robot associated complications in the live model. Whereas all abdominal procedures could be performed successfully under general anesthesia, one piglet was euthanized early before the thoracic intervention, likely due to pulmonary inflammatory response. Technical limitations were based on the size of the camera (10 mm) being too large and the minimal insertion depth of the instruments for calibration of the pivot point.

CONCLUSIONS:

Robotic surgery on newborns and infants appears technically feasible with the Versius® system. Software adjustments for pivot point calibration need to be implemented by the manufacturer as a result of our study.
To further evaluate the Versius® system, prospective trials are needed, comparing comparing it to open and laparoscopic surgery as well as to other robotic systems.
Identification of STAT4 as a biomarker associated with tumor immunity and prognosis in acute myeloid leukemia
Authors: Zhao Jingyuan , Li Shuai , Tang Jingya , Tian Wei , Kang Bingyu , Chen Yanwei , Hong Yuan , Dong Deshi 
Published: November 2022

Abstract
Acute myelogenous leukemia (AML) is a common malignancy and is supposed to have the ability to escape host immune surveillance. This study aimed to identify key genes in AML that may affect tumor immunity and to provide prognosis biomarkers of AML. The Cancer Genome Atlas (TCGA)dataset was screened for transcription factors (TFs) involved in immunity and influencing survival, combining Gene Expression Omnibus (GEO) data to validate the impact on patient survival. A prognostic signature was established using four transcription factors, and these genes play an important role in the immune system, with higher regulatory T cell (Treg) scores in high-risk patients compared to the low-risk group. Analysis of individual genes showed that STAT4 and Treg are closely related, which may be due to STAT4 transcribing related genes that affect immunity. STAT4 expression was positively correlated with the proportion of abnormal cells and promoted AML recurrence as verified by AML clinical patient samples. In conclusion, these findings suggest that STAT4 may be a potential biomarker for AML prognosis. As a key gene affecting the prognosis of AML patients, STAT4 may be a therapeutic target.
Sertoli cell-only phenotype and scRNA-seq reveal hnRNPU as a regulator required for spermatogonial stem cell pool establishment in mice
Authors: Yuan Shuiqiao , Wen Yujiao , Zhou Shumin , Gui Yiqian , Zhao Chuansen , Gan Shiming , Feng Shenglei , Ma Xixiang , Yin Lisha , Xu Wenchao , Xiong Mengneng , Wang Xiaoli 
Published: November 2022

Abstract
The continuous regeneration of spermatogonial stem cells (SSCs) underpins spermatogenesis and lifelong male fertility; however, little is known about the developmental origins of the SSC pool. Here, we document that heterogeneous nuclear ribonucleoprotein U (hnRNPU) is essential for establishing the SSC pool. In male mice, conditional loss of hnRNPU in prospermatogonia (ProSG) arrests spermatogenesis and results in sterility, characterized by complete loss of germ cells around postnatal day 10, which resembles the Sertoli cell-only phenotype in humans. hnRNPU-deficient ProSG fails to differentiate and migrate to the basement membrane to establish SSC pool in infancy. Moreover, we find that the deletion of hnRNPU leads to the accumulation of ProSG and the reduction of undifferentiated spermatogonia and further disrupts the process of T1-ProSG to T2-ProSG transition. hnRNPU-deficiency in ProSG deregulates the expression of spermatogenic-related genes and destroys the alternative splicing of genes related to cell cycles, and single-cell transcriptional analyses reveal germ cells are in a mitotically quiescent state and lost their unique identity upon hnRNPU deletion. We further show that hnRNPU could interact with DDX5, SRSF3, and TRIM28 proteins and bind to Vrk1, Slx4, and Dazl transcripts with identified to be suffered aberrant alternative splicing in hnRNPU-deficient testes. These observations give important insights into SSC pool establishment and may have translational implications for male fertility.
3D printed models as an adjunct to traditional teaching of anatomy in congenital heart disease.
Authors: Tarca Adrian , Woo James , Bain Shahira , Crouchley David , McNulty Eamonn , Yim Deane 
Published: November 2022

Abstract
Background
Three-dimensional (3D) printed cardiac models are increasingly being utilised for medical education, simulation and training, communication, surgical planning and research. Due to the complexities of congenital cardiac anatomy, 3D printing is well suited as an adjunct to traditional teaching methods. We hypothesised that using 3D printed cardiac models as an adjunct to didactic teaching methods would improve knowledge and confidence levels of participants, regardless of their cardiology experience.
Methods
A prospective randomised study was performed recruiting paediatric nurses and doctors. All participants undertook traditional congenital cardiac teaching describing normal cardiac anatomy and seven congenital lesions of increasing complexity (atrial septal defect (ASD), ventricular septal defect (VSD), vascular ring, partial anomalous pulmonary venous return (PAPVR), Tetralogy of Fallot (TOF), transposition of the great arteries (TGA) and double outlet right ventricle (DORV)). The intervention group received an additional recorded demonstration, while handling 3D printed models of a normal heart and the same lesions. Pre and post-intervention assessments were completed using a subjective Likert scale questionnaire and objective multiple choice examination.
Results
Seventy-three health practitioners, (30 cardiac nurses and 43 paediatric trainees) were included. A significant increase in subjective confidence (mean score of 2.7 (SD 0.88) to 3.3 (0.71), increase of 0.58 vs 2.5 (1.0) to 2.6 (0.87), increase of 0.06, p<0.001) and knowledge (mean score of 2.5 (0.7) to 3.4 (0.64), increase of 0.8 vs 2.4 (0.94) to 2.6 (0.83), increase of 0.2, p=0.181) was observed in the intervention but not control group. A significantly larger increase in post-intervention examination score was observed in the intervention group (4.27 (21.4%) vs 2.28 (11.4%), p=0.002). There was no difference in pre-test score or post-test improvement based on previous experience.
Discussion
3D printed cardiac models significantly improve subjective and objective confidence towards and knowledge of a range of congenital cardiac lesions for both nurses and paediatric trainees. 3D cardiac models are a useful adjunct to traditional teaching methods as they overcome limitations with 2D representations of 3D structures, provide additional educational opportunities in a scarce learning environment and are consistent with adult learning principals. As barriers to production and access are overcome, 3D printed cardiac models should be incorporated more widely in medical education programs.
HeliDye1: helquat fluorogenic probe specific for AT-rich DNA duplexes
Authors: Kužmová Erika , Joshi Vishwas , Kozák Jaroslav , Severa Lukáš , Bednarova Lucie , Yurenko Yevgen , Dobiaš Juraj , Pecina Adam , Köprülüoğlu Cemal , Jurečka Petr , Lepšík Martin , Cisarova Ivana , Pohl Radek , Jurkiewicz Piotr , Cebecauer Marek , Trojanec Radek , Dzubak Petr , Hajduch Marian , Lizcová Libuše , Svobodová Karla , Zemanová Zuzana , Teplý Filip , Reyes-Gutiérrez Paul E. , Zawada Zbigniew 
Published: November 2022

Abstract
DNA-binding fluorescent probes are useful tools in cell biology for various applications enabling visualisation and quantification of DNA. Herein, we report on HeliDye1, a novel cationic helicene-like DNA probe, and its versatile usability. HeliDye1 exhibits fluorescence exclusively in the presence of DNA duplexes containing AT base pairs and (contrary to any recent DNA probe) it remains non-fluorescent in the presence of single-stranded DNA and RNA. Favourable chemical properties of HeliDye1 allowed its use in karyotyping and super-resolution microscopy. We present five more applications of HeliDye1. Viscosimetry showed that HeliDye1 is a minor groove binder. HeliDye1 interacts with double-stranded DNA as an induced (P)-enantiomer, which we confirmed by ECD spectroscopy and DFT calculations. ECD spectroscopy suggested the formation of HeliDye1 dimers. MD simulations predicted the structures of (P)-HeliDye1 dimers inside DNA and showed their stability in AT-rich sequences and instability in GC-rich sequences.
Heart Disease Diagnosis Using Optimized Features of Hybridized Alcsoga Algorithm and Lstm Classifier
Authors: K Kalaivani , N. Uma Maheswari , R Venkatesh 
Published: November 2022

Abstract
Background: Cardiac Disease is the predominant cause of global death mainly due to its hidden symptoms and late diagnosis. People with CVD along with other diseases like hypertension, hyperlipidemia require very early detection for appropriate treatment. Hence this research proposed a hybrid technique for heart disease diagnosis.
Objective The main contribution of the study is to overcome the existing limitations of Antlion, Crow search, and improved genetic algorithm and to hybridize the algorithm for the effective feature selection thereby improving the classification performance of the LSTM classifier. The motivation of this research is to improve the feature selection method with the optimization of features for effective heart disease prediction.
Methodology: The proposed architecture uses the Ant lion algorithm with the effective determination of the elite position. The crow search Algorithm utilizes the phenomenon of position and memory of each crow for the evaluation of objective function. The inputs were processed by the improved genetic algorithm for effective feature selection. Now the hybridized proposed system successfully extracts the optimized features. These features are classified by the LSTM classifier.
Results: The performance analysis was performed with two datasets. The dataset 1 had been used for the determination of the efficiency of the proposed system and the dataset 2 is utilized for the estimation of the proposed system followed by a detailed comparative analysis with the existing system. Further cross-validation of the sample with a varied range of testing percentage has also been accomplished. Apart from the intercombination performance of feature selection among the three utilized algorithms was also compared. From this comparative analysis, the proposed method had the highest accuracy of 99.7% compared to existing methods.
Investigating the Rural and Entrepreneurial Development through Microfinance
Authors: Parray Zahoor Ahmad , Rather Raouf Ahmad Ahmad , Bhat Shabir Ahmad , Ahmad Pirzada Sajad 
Published: November 2022

Microfinance has been evolving to be a decisive factor in poverty reduction, rural and entrepreneurial development. This research aims to find out the impact of microfinance on rural/socio-economic development and entrepreneurial skill development. Particularly, this study attempts to explore the impact of microfinance on social development, financial empowerment, education, healthcare, economic independence, and entrepreneurial development. Data was collected through questionnaires taken from the beneficiaries of the National Rural Livelihood Mission (NRLM) erstwhile Swaran Jayanti Shahri Rozgar Yojana (SJSRY) microfinance scheme in the Union Territory of Jammu and Kashmir, India. A purposive sampling method, exploratory factor analysis, and paired sample t-test were used in this study. The findings prove that microfinance facilitates social development, improved financial empowerment, and promotes decision-making relating to education and healthcare among beneficiaries. The finding also indicates that microfinance helps to increase entrepreneurial skill development among the beneficiaries. This study offers an insight how microfinance eliminates poverty and facilitates social development as well as entrepreneurial skills. These findings have essential implications for academics, policymakers, and microfinance institutions.