bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Saccharomyces eubayanus haploid resource for research studies
Authors: Molinet Jennifer , Urbina Kamila , Villegas Claudia , Abarca Valentina , Oporto Christian I. , Villarreal Pablo , Villarroel Carlos A. , Salinas Francisco , Nespolo Roberto F. , Cubillos Francisco A. 
Published: April 2022

AbstractSince its identification,Saccharomyces eubayanushas been recognized as the missing parent of the lager hybrid,S. pastorianus. This wild yeast has never been isolated from fermentation environments, thus representing an interesting candidate for evolutionary, ecological and genetic studies. However, it is imperative to develop additional molecular genetics tools to ease manipulation and thus facilitate future studies. With this in mind, we generated a collection of stable haploid strains representative of three main lineages described inS. eubayanus(PB-1, PB-2 and PB-3), by deleting theHOgene using CRISPR-Cas9 and tetrad micromanipulation. Phenotypic characterization under different conditions demonstrated that the haploid derivates were extremely similar to their parental strains. Genomic analysis in three strains highlighted a likely low frequency of off-targets, and sequencing of a single tetrad evidenced no structural variants in any of the haploid spores. Finally, we demonstrate the utilization of the haploid set by challenging the strains under mass-mating conditions. In this way, we found thatS. eubayanusunder liquid conditions has a preference to remain in a haploid state, unlikeS. cerevisiaethat mates rapidly. This haploid resource is a novel set of strains for future yeast molecular genetics studies.
Impact of experience on visual behavior and driving performance of high-speed train drivers
Authors: Du Yang , Zhi Jin-yi , He Si-jun 
Published: April 2022

AbstractThis study investigated the impact of experience on the visual behavior and driving performance of high-speed train drivers, and explored the correlation between visual behavior and driving performance. Through a simulated driving task, eye movement data and operating data of novice drivers, trainee drivers, and experienced drivers in the traction stage, normal operation process stage, and braking stage were collected. Variance and linear regression were used to analyze the difference and correlation between indicators. The results show that experience could change the driver’s information collection method from long fixation to multi-frequency. Experience also increased the consistency of group operations and reduced the likelihood of hazard occurrences. Therefore, driving performance can be improved by reducing the average fixation duration of information through interface optimization.
The Effects of Intensive Voice Treatment in Mandarin Speakers With Parkinson's Disease: Acoustic and Perceptual Findings
Authors: Hsu Sih-Chiao , Jiao Yishan , Berisha Visar , McAuliffe Megan J. , Lin Peiyi , Wu Ruey-Meei , Cheng Shih-Jung , Levy Erika S. 
Published: April 2022

Purpose:
This study investigated the effects of intensive voice treatment on subjective and objective measures of speech production in Mandarin speakers with hypokinetic dysarthria.

Method:

Nine Mandarin speakers with hypokinetic dysarthria due to Parkinson's disease received 4 weeks of intensive voice treatment (4 × 60 min per week). The speakers were recorded reading a passage before treatment (PRE), immediately after treatment (POST), and at 6-month follow-up (FU). Listeners (
n
= 15) rated relative ease of understanding (EOU) of paired speech samples on a visual analogue scale. Acoustic analyses were performed. Changes in EOU, vocal intensity, global and local fundamental frequency (
f
o
) variation, speech rate, and acoustic vowel space area (VSA) were examined.

Results:

Increases were found in EOU and vocal intensity from PRE to POST and from PRE to FU, with no change found from POST to FU. Speech rate increased from PRE to POST, with limited evidence of an increase from PRE to FU and no change from POST to FU. No changes in global or local
f
o
variation or in VSA were found.

Conclusions:

Intensive voice treatment shows promise for improving speech production in Mandarin speakers with hypokinetic dysarthria. Vocal intensity, speech rate, and, crucially, intelligibility, may improve for up to 6 months posttreatment. In contrast,
f
o
variation and VSA may not increase following the treatment.

Supplemental Material:

https://doi.org/10.23641/asha.19529017
Voice and Speech Changes in Transmasculine Individuals Following Circumlaryngeal Massage and Laryngeal Reposturing
Authors: Dahl Kimberly L. , François Felicia A. , Buckley Daniel P. , Stepp Cara E. 
Published: April 2022

Purpose:The purpose of this study was to measure the short-term effects of circumlaryngeal massage and laryngeal reposturing on acoustic and perceptual characteristics of voice in transmasculine individuals.Method:Fifteen transmasculine individuals underwent one session of sequential circumlaryngeal massage and laryngeal reposturing with a speech-language pathologist. Voice recordings were collected at three time points—baseline, postmassage, and postreposturing. Fundamental frequency (fo), formant frequencies, and relative fundamental frequency (RFF; an acoustic correlate of laryngeal tension) were measured. Estimates of vocal tract length (VTL) were derived from formant frequencies. Twelve listeners rated the perceived masculinity of participants' voices at each time point. Repeated-measures analyses of variance measured the effect of time point onfo, estimated VTL, RFF, and perceived voice masculinity. Significant effects were evaluated with post hoc Tukey's tests.Results:Between baseline and end of the session,fodecreased, VTL increased, and participant voices were perceived as more masculine, all with statistically significant differences. RFF did not differ significantly at any time point. Outcomes were highly variable at the individual level.Conclusion:Circumlaryngeal massage and laryngeal reposturing have short-term effects on select acoustic (fo, estimated VTL) and perceptual characteristics (listener-assigned voice masculinity) of voice in transmasculine individuals.Supplemental Material:https://doi.org/10.23641/asha.19529299
Daily Phonotrauma Index: An Objective Indicator of Large Differences in Self-Reported Vocal Status in the Daily Life of Females With Phonotraumatic Vocal Hyperfunction
Authors: Nudelman Charles J. , Ortiz Andrew J. , Fox Annie B. , Mehta Daryush D. , Hillman Robert E. , Van Stan Jarrad H. 
Published: April 2022

Purpose:
The purpose of this study is to evaluate if the Daily Phonotrauma Index (DPI) can quantitatively discriminate large differences in overall vocal status in the daily life of patients with phonotraumatic vocal hyperfunction (PVH).

Method:
For 1–4 weeks, 23 females with PVH wore an ambulatory voice monitor and answered three vocal status questions (i.e., difficulty producing soft, high-pitched phonation; discomfort; and fatigue) at the beginning, at 5-hr intervals, and the end of each day. DPI values were obtained for each patient's time periods of worst and best self-rated vocal status, and data for the group were analyzed for significant changes using a linear mixed-effects regression model.

Results:

The DPI was significantly lower during periods self-rated as “best vocal status” compared to during periods self-rated as “worst vocal status” (mean difference in DPI = 0.53) with a medium-to-large effect size (Cohen's
d
= −0.68).

Conclusions:
In a group of patients with phonotraumatic lesions, the DPI indicated lower potential for phonotrauma during time periods of better vocal status compared to time periods of worse vocal status. Assuming that a large portion of variance in vocal status for patients with PVH is associated with the extent to which voicing is phonotraumatic, these results support the validity of obtaining estimates of DPI for much shorter time periods (i.e., an estimate every 2 min of voicing) than previous studies (i.e., a single estimate for the entire day or week). Future work can investigate the DPI's use for in-clinic assessment/treatment and ambulatory biofeedback and can gain further insights into phonatory mechanisms that underlie DPI via comparisons with other physiologically relevant measures and computational vocal fold modeling.
Differential space time media‐based modulation system
Authors: Zou Hongliang , Quan Daying , Jin Xiaoping , Zhou Yueru 
Published: April 2022

AbstractDifferential spatial modulation (DSM) is a multi‐antenna differential technology with low power consumption and no demand of channel estimation. It is suitable for communication systems that have fast migration velocity and high requirements for both power consumption and complexity. However, due to the sparse structure and the requirements of differential coding, the spectral efficiency is limited. Therefore, media‐based modulation (MBM) technology is introduced into differential system in this work to improve the spectral. A differential space time media‐based modulation (DSTMBM) system is proposed. While retaining the inherent advantages of the DSM, the additional bits are transmitted through the radio frequency (RF) mirrors in DSTMBM. Unlike traditional DSM, the transmit antenna of DSTMBM system can be activated multiple times. In addition, a theoretical performance is derived, and a low‐complexity (LC) detection algorithm is adopted. Simulation results show that the DSTMBM system has a performance advantage of about 4.5 dB at a high transmission rate compared to traditional DSM systems. Monte Carlo simulation matches the theoretical performance in a wide range. Meanwhile, the LC detection algorithm reduces the complexity by 2–3 orders of magnitude without the bit error rate (BER) performance loss.
Hardware routed quantum key distribution networks
Authors: Singal Ansh , Iyengar Sundaraja Sitharam , Kumar Latesh , Madni Azad M. 
Published: April 2022

AbstractQuantum communication networks pose immense potential for revolutionising secure communications for several applications such as banking, defence, etc. The majority literature on quantum networks deals with the problems of networking and resource allocation using Software Defined Networks (SDNs). SDNs, however, introduce several issues, such as app manipulation attacks and scalability issues. We propose a novel scheme of implementing quantum communication networks that are hardware routed rather than software defined by labelling qubit photons using laser communications. We provide a comprehensive implementation of the new scheme and propose two novel algorithms—Bandwidth sharing and Equitable bandwidth sharing to implement the hardware routed quantum network. The algorithms result in a key rate increase of 118% and improved network resource utilization of 147% as compared to the First Come First Serve algorithm.
Submillimeter wave spectroscopy and astronomical search for 1-propanimine
Authors: Margulès L. , Remijan A. , Belloche A. , Motiyenko R. A. , McGuire B. A. , Xue C. , Müller H. S. P. , Garrod R. T. , Menten K. M. , Guillemin J.-C. 
Published: April 2022

Context.Methanimine, CH2NH, and ethanimine, CH3CHNH, were both detected in the interstellar medium (ISM), the former in the 1970s and the latter nearly a decade ago. By analogy with nitriles, for which hydrogen cyanide, acetonitrile, and ethyl cyanide are the most abundant, n-propanimine was suggested as a candidate for detection in the ISM.Aims.To date, no measurements of the rotational spectrum of 1-propanimine have been made. We present measurements and an analysis of its spectrum in the millimeter- and submillimeter-wave domain in order to provide an accurate prediction that is required to search for this molecule in the ISM.Methods.The rotational spectrum of propanimine was investigated from 150 to 470 GHz. Watson’s asymmetric top Hamiltonian in theIrrepresentation and S reduction was used for the analysis. The new prediction allowed us to search for 1-propanimine toward the high-mass star forming region Sagittarius B2(N), the dark molecular cloud TMC-1, as well as a sample of low-mass protostellar objects. These sources were observed with the Atacama Large Millimeter/submillimeter Array, the Green Bank Telescope, or the IRAM 30 m telescope.Results.A total of 585 and 296 transitions of the E and Z-1-propanimine, respectively, were newly assigned and fitted in the laboratory spectra recorded up to 470 GHz. Quantum numbers up toJ= 73 andKa= 18 were reached for theEisomer andJ= 56 andKa= 17 for the Z isomer. We report the nondetection of 1-propanimine toward all astronomical sources investigated in this work. We find in particular that 1-propanimine is at least 20 times less abundant than methanimine in the envelope of the high-mass star forming region Sagittarius B2(N).Conclusions.The accurate spectroscopic prediction of its spectrum provided in this work will allow astronomers to continue the search for 1-propanimine in the ISM.
Syphilisinfektion im HNO-Bereich
Authors: Seuthe Inga Marte Charlott , Eichhorn Sabine , Kim Jonghui , van Ackeren Konstantin , Park Jonas Jae-Hyun , Dazert Stefan , Klespe Kai-Christian 
Published: April 2022

ZusammenfassungDie Anzahl der gemeldeten Syphilis-Fälle steigt seit Jahren an. Die sexuell übertragbare Erkrankung wird durch die Spirochäte Treponema pallidum Subspezies pallidum verursacht und verläuft hierbei in verschiedenen Stadien. In allen Stadien können hierbei Symptome im HNO-Bereich auftreten. Hierdurch ist bei passenden Symptomen eine Syphilis-Infektion als Differenzialdiagnose vom HNO-Arzt stets zu bedenken. So ist bei zunehmendem oralem Geschlechtsverkehr der Primäraffekt/harter Schanker zunehmend häufig im Mundraum zu beobachten. Zudem können Symptome nicht nur im Mundraum, sondern auch im Bereich des Ohrs, der Nase, des Larynx sowie auch zervikal und fazial auftreten. Die Diagnose wird hierbei über den direkten Erregernachweis oder über einen serologischen Nachweis gesichert. Die Spirochäte ist nicht kultivierbar. Der therapeutische Goldstandard ist die Gabe von Benzathin-Penicillin G oder Procain-Penicillin G. Als Alternative stehen Doxycyclin, Makrolide oder Ceftriaxon zur Verfügung. Bei einer Innenohr- oder Hirnnervenbeteiligung ist die zusätzliche Gabe eines Glukokortikoids zu empfehlen. Vor Durchführung der antibiotischen Therapie ist der Patient über die Möglichkeit einer Jarisch-Herxheimer-Reaktion aufzuklären. Bei einer Syphilis-Infektion ist stets eine kollegiale Zusammenarbeit mit den Venerologen zu empfehlen, sodass eine effektive und umfassende Diagnostik und Therapie erfolgen kann.
A critical evaluation of rationalist IR in the analysis of informal institutions
Authors: Cooper Andrew F. 
Published: April 2022

AbstractThis article has two major inter-connected objectives about the ascendancy of informal international institutions on a global basis. The first objective is to highlight the extended scope of this phenomenon. The second objective of this article evaluates how the mainstream International Relations (IR) literature has treated the cascading wave of informal institutions. With this expansion in mind, the focus is on a critical evaluation of the rationalist institutionalist literature generally and rational design scholarship more specifically. This scholarship possesses some considerable foundational advantages. At the same time several important deficiencies stand out. First, in terms of participation, the scholarship remains excessively US-centric. Secondly, in terms of projection, the rationalist institutionalist literature lacks both historical context and an anticipatory component. Thirdly, in terms of the ‘living personality’ constitutive of informal institutions, the mode of analysis lacks nuance. Notwithstanding its claims of consistency with respect to the logic of institutionalism, the rationalist institutionalist literature is highly uneven in terms of its analysis about the nature of informal institutions. The most consistent component throughout rationalist institutionalist literature is what type of informal institutionalism is left out. Informal institutionalism is recognized to be on the ascendancy. But the core manifestation of informal institutionalism—state-based plurilateralism—is neglected in the analysis.
One-kilojoule pulsed-power generator for laboratory space sciences
Authors: Chang Po-Yu , Lin Yen-Cheng , Kuo Ming-Hsiang , Du Cheng-Han , Hsieh Chih-Jui , Huang Mei-Feng , Jheng Ming-Cheng , Liu Jia-Kai , Yang Sheng-Hua , Yeh I-Lin , Wessel Frank J. 
Published: April 2022

This paper reports on the assembly of a compact, low-cost, pulsed-power facility used for plasma studies. The construction uses two modules placed on opposite sides of the test chamber to minimize the system impedance and improve access to test samples. The stored energy is 1 kJ with a peak current of 135 kA and a 1592 ns quarter-period time. Up until now, an imploding conical-wire array has been studied by using time-integrated (visible) imaging, and time-resolved laser imaging, providing a measure of the plasma jet speed in the range of 170 km/s. Our future plans will continue to investigate high-energy-density plasmas that are relevant to the space environment, fusion, and spectroscopy.
Ultrafast excitation quenching by the oxidized photosystem II reaction center
Authors: Akhtar Parveen , Sipka Gábor , Han Wenhui , Li Xingyue , Han Guangye , Shen Jian-Ren , Garab Győző , Tan Howe-Siang , Lambrev Petar H. 
Published: April 2022

Photosystem II (PSII) is the pigment–protein complex driving the photoinduced oxidation of water and reduction of plastoquinone in all oxygenic photosynthetic organisms. Excitations in the antenna chlorophylls are photochemically trapped in the reaction center (RC) producing the chlorophyll–pheophytin radical ion pair P+ Pheo−. When electron donation from water is inhibited, the oxidized RC chlorophyll P+ acts as an excitation quencher, but knowledge on the kinetics of quenching is limited. Here, we used femtosecond transient absorption spectroscopy to compare the excitation dynamics of PSII with neutral and oxidized RC (P+). We find that equilibration in the core antenna has a major lifetime of about 300 fs, irrespective of the RC redox state. Two-dimensional electronic spectroscopy revealed additional slower energy equilibration occurring on timescales of 3–5 ps, concurrent with excitation trapping. The kinetics of PSII with open RC can be described well with previously proposed models according to which the radical pair P+ Pheo− is populated with a main lifetime of about 40 ps, which is primarily determined by energy transfer between the core antenna and the RC chlorophylls. Yet, in PSII with oxidized RC (P+), fast excitation quenching was observed with decay lifetimes as short as 3 ps and an average decay lifetime of about 90 ps, which is shorter than the excited-state lifetime of PSII with open RC. The underlying mechanism of this extremely fast quenching prompts further investigation.
Multidimensional political apportionment
Authors: Cembrano Javier , Correa José , Verdugo Victor 
Published: April 2022

Significance
A cornerstone in the modern political organization of societies is the existence of a deliberative assembly, reflecting the needs of different population segments. As modern societies become more complex, representation according to dimensions beyond political affiliation and geography is demanded; examples include gender balance and ethnicity. As this dimensionality increases, the task becomes more challenging and requires more sophisticated mathematical tools. In this paper, we initiate the study of multidimensional apportionments and show that, in three and more dimensions, their existence is not guaranteed. However, our main result states that it is possible to elect a house nearly respecting proportionality of representation along several dimensions simultaneously. We finally illustrate the potential of our approach with recent election data.
FKBP52 and FKBP51 differentially regulate the stability of estrogen receptor in breast cancer
Authors: Habara Makoto , Sato Yuki , Goshima Takahiro , Sakurai Masashi , Imai Hiroyuki , Shimizu Hideyuki , Katayama Yuta , Hanaki Shunsuke , Masaki Takahiro , Morimoto Masahiro , Nishikawa Sayaka , Toyama Tatsuya , Shimada Midori 
Published: April 2022

Significance
Estrogen receptor α (ERα) is a transcription factor that induces cell proliferation and exhibits increased expression in a large subset of breast cancers. We comprehensively searched for indicators of poor prognosis in ERα-positive breast cancer through the multiple databases, including interactome, transcriptome, and survival analysis, and identified FKBP52. We found that two immunophilins, FKBP52 and FKBP51, have opposing effects on ERα stability and propose that therapeutic targeting of FKBP52 could be useful for the prevention and treatment of ERα-positive breast cancers, including endocrine therapy–resistant breast cancers.
Monocyte-derived SDF1 supports optic nerve regeneration and alters retinal ganglion cells’ response to Pten deletion
Authors: Xie Lili , Cen Ling-Ping , Li Yiqing , Gilbert Hui-Ya , Strelko Oleksandr , Berlinicke Cynthia , Stavarache Mihaela A. , Ma Madeline , Wang Yongting , Cui Qi , Kaplitt Michael G. , Zack Donald J. , Benowitz Larry I. , Yin Yuqin 
Published: April 2022

Significance
The optic nerve conveys information from retinal ganglion cells (RGCs) to visual processing areas of the brain. Although this pathway normally cannot regenerate when injured nor in degenerative diseases such as glaucoma, this failure can be partially reversed by eliciting a controlled immune reaction in the eye. We show here that the chemokine SDF1 (stromal cell–derived factor 1) is an important contributor to this phenomenon. SDF1 is produced by infiltrative monocytes and acts through its cognate receptor to enhance RGC survival, promote optic nerve regeneration, and sensitize subtypes of RGCs that normally fail to respond to a complementary treatment to exhibit robust, long-distance regeneration. These findings establish SDF1 as an important therapeutic candidate for repairing the injured optic nerve.
Workplace inequality is associated with status-signaling expenditure
Authors: Muggleton Naomi , Trendl Anna , Walasek Lukasz , Leake David , Gathergood John , Stewart Neil 
Published: April 2022

Significance
Scholars have identified that inequality is a notable detriment to well-being. Status-signaling luxury expenditure is taken as a symptom of the reduced well-being associated with income inequality. Despite evidence that status-signaling luxury expenditure is higher in unequal regions, it remains unclear who is affected by inequality. We use payroll and daily spending data from 683,677 individuals in 32,008 precisely-defined workplace peer groups to show that workers at unequal firms spend significantly more on high-status, luxury goods. This is also seen in those with a high absolute salary, but low salary rank within their workplace. Compared to aggregated, regional data, financial data allow us to identify groups of workplace peers and offer precise measurements of status-signaling expenditure for each individual.
Glycosaminoglycans modulate long-range mechanical communication between cells in collagen networks
Authors: Chen Xingyu , Chen Dongning , Ban Ehsan , Toussaint Kimani C. , Janmey Paul A. , Wells Rebecca G. , Shenoy Vivek B. 
Published: April 2022

Significance
Glycosaminoglycans (GAGs) are carbohydrates that are expressed ubiquitously in the human body and are among the key macromolecules that influence the development, homeostasis, and pathology of native tissues. Abnormal accumulation of GAGs has been observed in metabolic disorders, solid tumors, and fibrotic tissues. Here we theoretically and experimentally show that tissue swelling caused by the highly polar nature of GAGs significantly affects the mechanical interactions between resident cells by altering the organization and alignment of the collagenous extracellular matrix. The role of GAGs in modulating cellular force transmission revealed here can guide the design of biomaterial scaffolds in regenerative medicine and provides insights on the role of cell–cell communication in tumor progression and fibrosis.