bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cribriform pattern in prostate tissues: Predictor for intraductal carcinoma of the prostate based on biopsy and radical prostatectomy pathology
Authors: Shiomi Ei , Kato Renpei , Kanehira Mitugu , Takata Ryo , Sugimura Jun , Nakamura Yasuyuki , Ujiie Takashi , Abe Takaya , Obara Wataru 
Published: October 2022

AbstractObjectivesThis study aims to investigate whether a cribriform pattern on prostate biopsy may be a factor in suspicion of intraductal carcinoma of the prostate after radical prostatectomy.MethodsThis retrospective study assessed 100 men who underwent prostatectomy from 2015 to 2019. Participants were grouped as 76 patients with Gleason pattern 4 and 24 patients without this pattern. All 100 participants underwent retrograde radical prostatectomy and limited lymph node dissection. The same pathologist evaluated all specimens. The cribriform pattern was evaluated with haematoxylin and eosin counterstaining, and intraductal carcinoma of the prostate was evaluated with immunohistochemical analysis of cytokeratin 34βE12.ResultsPatients with intraductal carcinoma of the prostate on immunohistochemical analysis showed a significant tendency to relapse in the postoperative period, and those with the cribriform pattern on biopsy had a significant recurrence rate. In univariate and multivariate analyses, intraductal carcinoma of the prostate confirmed in biopsy tissue was an independent predictor of biochemical recurrence after prostatectomy. The rate of intraductal carcinoma of the prostate confirmation was 28% of cases with a cribriform pattern in biopsy tissue, which was increased to 62% in prostatectomy tissues.ConclusionThe cribriform pattern in the biopsy tissue may be a predictor for intraductal carcinoma of the prostate.
A strategy to explore the quality markers of Ziziphi Spinosae semen by combining metabolic in vivo study with network pharmacology
Authors: Yan Yan , Liu Jiaxing , Zhang Min , Zhang Yinjie , Shi Biyun , Qin Xuemei , Du Chenhui 
Published: October 2022

AbstractZiziphi Spinosae semen (ZSS), the dried and ripe seed of Ziziphus jujube Mill. var. spinosa (Bunge) Hu ex H. F. Chou, has been used as a sedative in China and other Asian countries for over a millennium. However, its quality markers (Q‐markers) are not completely clear. In this study, Q‐markers selected by a metabolic in vivo study combined with network pharmacology are proposed for ZSS quality control. An UHPLC (ultra‐high‐performance liquid chromatography)‐Q‐Orbitrap‐MS method was developed to identify or tentatively assign 48 components including 21 flavonoid C‐glycosides, 2 flavonoid O‐glycosides, 11 dammarane triterpenoid saponins, 13 alkaloids, and 1 other, using a diagnostic product ion filtering strategy in ZSS. Subsequently, 147 metabolites detected from serum, urine, bile, and feces samples of para‐chlorophenylalanine–induced insomnia rats treated with ZSS aqueous extracts could be linked to their respective parent compounds, including 27 prototypes. Meanwhile, three metabolic networks of flavonoids, saponins, and alkaloids are preliminarily established and potential metabolic pathways are investigated under the insomnia condition. Finally, 12 key bioactive components against insomnia including magnoflorine, caaverine, coclaurine, norisocorydine, genkwanin, juzinrine, apigenin, jujubogenin, kaempferol‐3‐O‐rutinoside, jujuboside A, jujuboside B, and spinosin with the highest degree values in component–target–pathways network were selected as Q‐markers for the quality control of ZSS.
Development of a UPLC–MS/MS method for the determination of active ingredients of Shuang Hu in rat blood and its application in pharmacokinetics
Authors: Jiang Xin Rui , Lei Meng Ying , Qin Liu Xin , Zhou Yan Lin , Wang Yang , Li Xue Jian , Wang Gang 
Published: October 2022

AbstractA simple and sensitive method using in vivo microdialysis coupled with UPLC–MS/MS was established to evaluate the pharmacokinetics of Shuang Hu tincture (SHZTN). Xevo TQ‐S was used to analyze the active ingredients of mesaconitine, hypaconitine, 4‐hydroxycinnamic acid, ferulic acid and N‐(2, 3‐dimethyl phenyl)‐2‐ aminobenzoic acid of SHZTN. Samples were prepared using a methanol precipitation method and the internal standards lannaconitine and p‐hydroxybenzoic acid were added. The method validation was conducted according to the guidelines of the Pharmacopoeia of China. A good linear range was obtained in the range of 1–2,000 ng/ml. The intra‐day and inter‐day precisions were less than 14.7%, and the accuracy range of all the analytes was −10.5–9.3%. The recovery of each analyte was over 95.5%, and matrix effects can be neglected. After a single dose of 20 mg/kg SHZTN, the area under the curve and peak concentration of the five active ingredients were significantly increased by transdermal compared with oral administration, which indicated the high bioavailability of SHZTN. The time to peak concentration of all compounds was <3.4 h, and the half‐life was <15.4 h, which indicated that the five compounds have the best absorption and rapid elimination. The method was successfully developed and applied to the pharmacokinetic study of SHZTN.
Statin use in patients with hormone receptor‐positive metastatic breast cancer treated with everolimus and exemestane
Authors: Lee Kyoungmin , Noh Eunjin , Moon Seok Joo , Joo Yoonjung Yoonie , Kang Eun Joo , Seo Jae Hong , Park In Hae 
Published: October 2022

AbstractBackgroundWe analyzed the effect of statins in patients with hormone receptor‐positive (HR+) metastatic breast cancer treated with everolimus + exemestane (EverX).Materials and MethodsWe conducted a nationwide retrospective cohort study using the National Health Insurance database with patients who received EverX for metastatic breast cancer between 2011 and 2019.ResultsOf 224,948 patients diagnosed with breast cancer, 1749 patients who received EverX for at least 30 days were included. Among them, 500 (28.6%) patients were found to take statins with EverX treatment (statin group), and the median duration of this combination was 5.36 months. The median time to treatment duration (TTD) for EverX and the overall survival (OS) were significantly higher in the statin group than in the no‐statin group [7.69 vs. 5.06 months, p < 0.001; 45.7 vs. 26.0 months, p < 0.001, respectively]. Multivariable Cox analysis revealed that the use of statins was associated with prolonged TTD [HR = 0.67 (95% CI, 0.59–0.77)] and OS [HR = 0.57 (95% CI, 0.46–0.70)] for EverX even after adjustment for other covariates.ConclusionStatins may have synergistic effects with endocrine therapy with the mTOR inhibitor everolimus, and improve survival in patients with HR+ metastatic breast cancer.
Bartonella henselae vertebral osteomyelitis in a pediatric patient: A case report
Authors: Abu‐Rumeileh Sarah , Beltrami Giovanni , Di Maurizio Marco , Indolfi Giuseppe , Trapani Sandra 
Published: October 2022

AbstractBartonella henselae is an unusual and rarely suspected osteomyelitis etiologic agent. We present a case of low back‐pain in a 10‐year‐old female which lead to a challenging diagnostic work‐up due to subtle imaging findings. The diagnosis was Bartonella henselae vertebral osteomyelitis mimicking bone tumor.
Sumatriptan poisoning and its clinical presentation in humans: A case report
Authors: Erfan Talab Evini Peyman , Ohadi Laya , Amiri Farbod , Shadnia Shahin , Mostafazadeh Babak , Forouzesh Mehdi , Hedayatshodeh Mohammad Javad , Rahimi Mitra 
Published: October 2022

AbstractThis case report displays some of the possible complications of sumatriptan poisoning, including nephritic syndrome.
Evaluation of toxicities for intravesical drugs in phase 1 bladder cancer trials
Authors: Doersch Karen M. , Tabayoyong William B. , Bandari Jathin 
Published: October 2022

AbstractBackgroundImproving clinical trial design is important for optimizing approval of safe and effective drugs. Phase 1 clinical trials seek to determine phase 2 doses by investigating predefined dose‐limiting toxicities. Traditional definitions of dose‐limiting toxicity may not be applicable to intravesical therapies for bladder cancer. This study compared the frequency of dose‐limiting toxicities and serious adverse events in bladder cancer trials for intravesical therapies to other routes of administration.MethodsStudies were abstracted from ClinicalTrials.gov and reconciled with a PubMed search. Primary and secondary end points were predefined before data abstraction, and the primary end point was subject–level dose‐limiting toxicity rate. Fisher exact tests were performed with p < .05 designated as significant.ResultsEighteen intravesical studies and 24 studies with other routes of administration (the per os/intravenous/intramuscular [PO/IV/IM] group) were identified. Dose‐limiting toxicities were reported in 38.9% of intravesical studies, affecting 3.29% of subjects, compared with 30.0% of PO/IV/IM studies representing 4.19% of subjects (p = .52 for study‐level and p = .60 for subject‐level comparisons). Serious adverse events occurred in 53.9% of intravesical studies in 10.3% of subjects versus 91.0% of studies reporting serious adverse events affecting 41.4% of subjects in the PO/IV/IM group (p = .03 for subject‐level and p < .0001 for study‐level comparisons).ConclusionsThere was no difference in subject–level dose‐limiting toxicity rate between intravesical and PO/IV/IM bladder cancer trials. The serious adverse event rate was lower in the intravesical group. Heterogeneity of dose‐limiting toxicity definition may affect interpretation of toxicity in phase 1 bladder cancer clinical trials studying different routes of administration.Lay summary

Bladder cancer is a common cancer type that may be treated with therapies that are instilled into the bladder and act locally, called intravesical therapies.
This study used publicly available regulatory data from early phase clinical trials to determine whether measures of tolerability used in clinical trials are applicable to intravesical therapies for bladder cancer.
Molecular diagnostics of infectious disease: Detection and characterization of microbial agents in cytology samples
Authors: Caruso Carla R. , Yang Zhongbo 
Published: October 2022

AbstractBackgroundCytology samples are widely used to diagnose various infectious diseases by detection and identification of causative infectious agents, including bacteria, fungi, and viruses. The role of cytopathology in infectious disease has expanded tremendously in the past decades with the advances in molecular techniques. Molecular diagnostic methods, compared to conventional methods, have shown improved patient outcome, reduction in cost, and shortened hospital stay times. The aim of this article is to review molecular testing in cytology samples for diagnosis of infectious diseases.MethodsThe literature search for molecular testing in common cytology samples for diagnosis of infectious diseases was performed. The findings of the studies were summarized. The common cytology samples included in this article were gynecologic specimens, cerebrospinal fluid, bronchoalveolar lavage, and urine samples.ConclusionsThere are a number of molecular diagnostic tests that are available to be used in common cytology samples to detect infectious agents. Each test has its own advantages and limitations. It is our hope that upon reading this review article, the readers will have better understanding of molecular diagnostic testing of infectious diseases utilizing commonly sampled cytology specimens in daily practice.
Internalization of muscularity and thinness ideals: Associations with body dissatisfaction, eating disorder symptoms, and muscle dysmorphic symptoms in at risk sexual minority men
Authors: Convertino Alexandra D. , Elbe Connor I. , Mendoza Rebecca R. , Calzo Jerel P. , Brown Tiffany A. , Siegel Jaclyn A. , Jun Hee‐Jin , Corliss Heather L. , Blashill Aaron J. 
Published: October 2022

AbstractObjectiveIn the tripartite influence model, appearance‐ideal internalization is identified as a prominent risk factor for the development of body dissatisfaction and subsequent eating disorder (ED) behaviors. For men, prior research has emphasized the importance of both thin‐ideal internalization and muscular‐ideal internalization in explaining later ED behaviors and muscle dysmorphia (MD) symptoms. Previous research in heterosexual men has shown that the associations between muscular‐ideal internalization and ED or MD symptoms may depend on whether the individual has also internalized the thin ideal. However, this interaction has not been examined in research with sexual minority men (SMM).MethodThe current study collected self‐report data from 452 at risk SMM (i.e., endorsed body dissatisfaction), with ages ranging from 18 to 35 years. Linear regression models were conducted to test the interaction effects between thinness and muscularity internalization on ED symptoms, MD behaviors, and general body dissatisfaction. Simple slopes and the Johnson–Neyman technique were used to investigate significant interaction terms.ResultsThin‐ and muscular‐ideal internalization were positively associated with muscular appearance intolerance and dietary restriction with no significant interaction. Muscular drive for size was highest when both muscularity internalization and thinness internalization were high. Muscular‐ideal internalization was positively associated with both cognitive restraint and general body dissatisfaction, but only at lower levels of thinness internalization.DiscussionGiven the interacting association between thinness and muscularity internalization and aspects of body dissatisfaction, attitudes, and behavior, prevention and intervention programs for EDs and MDs in SMM should seek to dismantle both thinness and muscularity internalization.Public Significance StatementInternalizing—or adopting as one's own—the ideal of a body with low body fat and high muscularity has been shown to lead to muscle dysmorphia and eating disorder symptoms in men. The current study examines whether the combination of thin‐ideal and muscular‐ideal internalization is associated with worse symptoms than either facet alone in sexual minority men. Treatment efforts in sexual minority men should address both types of internalization.
How does the increase in eating difficulties according to the Development and Well‐Being Assessment screening items relate to the population prevalence of eating disorders? An analysis of the 2017 Mental Health in Children and Young People survey
Authors: O'Logbon Jessica , Newlove‐Delgado Tamsin , McManus Sally , Mathews Frances , Hill Suzanne , Sadler Katharine , Ford Tamsin 
Published: October 2022

AbstractObjectiveWe examine the test accuracy of the Development and Well‐Being Assessment (DAWBA) eating disorder screening items to explore whether the increased eating difficulties detected in the English National Mental Health of Children and Young People (MHCYP) Surveys 2021 reflect an increased population prevalence.MethodsStudy 1 calculated sensitivity, specificity, and positive and negative predictive values from responses to the DAWBA screening items from 4057 11–19‐year‐olds and their parents, in the 2017 MHCYP survey. Study 2 applied the positive predictive value to data from 1844 11–19‐year‐olds responding to the 2021 follow‐up to estimate the prevalence of eating disorders in England compared to 2017 prevalence.ResultsParental report most accurately predicted an eating disorder (93.6%, 95% confidence interval: 92.7–94.5). Sensitivity increased when parent and child answers were combined, and with a higher threshold (of two) for children. The prevalence of eating disorders in 2021 was 1% in 17–19‐year‐olds, and .6% in 11–16‐year‐olds—similar to the prevalence reported in 2017 (.8% and .6%, respectively). However, estimates for boys (.2%–.4%) and young men (.0%–.4%) increased.DiscussionWe found tentative evidence of increased population prevalence of eating disorders, particularly among young men. Despite this, the DAWBA screening items are useful for ruling out eating disorders, particularly when parents or carers screen negative, but are relatively poor at predicting who will have a disorder. Data from both parents and children and applying a higher cut point improves accuracy but at the expense of more missed cases.Public Significance StatementThe prevalence of eating disorders did not markedly change from 2017 to 2021, but we found tentative evidence of an increase, particularly among young men. This is despite larger increases in problematic eating, which need further investigation. The DAWBA screen is best suited to ruling out eating disorders which limits its clinical applications as it would provide many false positives requiring further assessment.
Defining the Biologically Plausible Taxonomic Domain of Applicability of an Adverse Outcome Pathway: A Case Study Linking Nicotinic Acetylcholine Receptor Activation to Colony Death
Authors: Jensen Marissa A. , Blatz Donovan J. , LaLone Carlie A. 
Published: October 2022

Abstract
For the majority of developed adverse outcome pathways (AOPs), the taxonomic domain of applicability (tDOA) is typically narrowly defined with a single or a handful of species. Defining the tDOA of an AOP is critical for use in regulatory decision-making, particularly when considering protection of untested species. Structural and functional conservation are two elements that can be considered when defining the tDOA. Publicly accessible bioinformatics approaches, such as the Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool, take advantage of existing and growing databases of protein sequence and structural information to provide lines of evidence toward structural conservation of key events (KEs) and KE relationships (KERs) of an AOP. It is anticipated that SeqAPASS results could readily be combined with data derived from empirical toxicity studies to provide evidence of both structural and functional conservation, to define the tDOA for KEs, KERs, and AOPs. Such data could be incorporated in the AOP-Wiki as lines of evidence toward biological plausibility for the tDOA. We present a case study describing the process of using bioinformatics to define the tDOA of an AOP using an AOP linking the activation of the nicotinic acetylcholine receptor to colony death/failure in Apis mellifera. Although the AOP was developed to gain a particular biological understanding relative to A. mellifera health, applicability to other Apis bees, as well as non-Apis bees, has yet to be defined. The present study demonstrates how bioinformatics can be utilized to rapidly take advantage of existing protein sequence and structural knowledge to enhance and inform the tDOA of KEs, KERs, and AOPs, focusing on providing evidence of structural conservation across species. Environ Toxicol Chem 2023;42:71–87. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Both noise‐floor and tissue compartment difference in diffusivity contribute to FA dependence on b‐value in diffusion MRI
Authors: Yao Junye , Tendler Benjamin C. , Zhou Zihan , Lei Hao , Zhang Lei , Bao Aimin , Zhong Jianhui , Miller Karla L. , He Hongjian 
Published: October 2022

AbstractNoninvasive diffusion magnetic resonance imaging (dMRI) has been widely employed in both clinical and research settings to investigate brain tissue microstructure. Despite the evidence that dMRI‐derived fractional anisotropy (FA) correlates with white matter properties, the metric is not specific. Recent studies have reported that FA is dependent on the b‐value, and its origin has primarily been attributed to either the influence of microstructure or the noise‐floor effect. A systematic investigation into the inter‐relationship of these two effects is however still lacking. This study aims to quantify contributions of the reported differences in intra‐ and extra‐neurite diffusivity to the observed changes in FA, in addition to the noise in measurements. We used in‐vivo and post‐mortem human brain imaging, as well as numerical simulations and histological validation, for this purpose. Our investigations reveal that the percentage difference of FA between b‐values (pdFA) has significant positive associations with neurite density index (NDI), which is derived from in‐vivo neurite orientation dispersion and density imaging (NODDI), or Bielschowsky's silver impregnation (BIEL) staining sections of fixed post‐mortem human brain samples. Furthermore, such an association is found to be varied with Signal‐to‐Noise Ratio (SNR) level, indicating a nonlinear interaction effect between tissue microstructure and noise. Finally, a multicompartment model simulation revealed that these findings can be driven by differing diffusivities of intra‐ and extra‐neurite compartments in tissue, with the noise‐floor further amplifying the effect. In conclusion, both the differences in intra‐ and extra‐neurite diffusivity and noise‐floor effects significantly contribute to the FA difference associated with the b‐value.
A numerical study of air entrapment during the droplet impact on a solid substrate
Authors: Umesh  , Singh N. K. 
Published: October 2022

AbstractA numerical investigation is conducted to study the air entrapment phenomenon when two different liquids such as water and diesel droplet are impacted on the solid surface. The beginning of the air entrapment process was observed during droplet impact on a solid substrate forming a dimple underneath the droplet. The air film thus trapped underneath the droplet started evolving into the air bubble. This journey of evolution mainly comprises phases like an inertial retraction of air film, contraction, and pinch‐off of the secondary droplet inside the air bubble for a water droplet impact case. The volume of fluid approach has been utilized to track the progress of air film evolution. The influence of surface wettability has been observed on the evolution of air film into the air bubble by taking four different values of contact angle pertaining to the hydrophilic surface (θ = 10° and 35°) and hydrophobic surface (θ = 90° and θ = 120°). The air bubble was found to get detached from the substrate for the hydrophilic surface (θ = 35°) and observed to remain attached to the substrate for the hydrophobic surface. The variation of pressure underneath the droplet was also investigated as the droplet reaches the substrate. The effect of surface tension has been studied on the evolution of air film by impacting the diesel droplet on the same substrate keeping the same wettability condition (θ = 35°). The lower surface tension of the diesel droplet as compared to the water droplet delayed the process of air film evolution and consequently decreases the retraction speed of air film. Also, the air bubble remains attached to the surface. Furthermore, the air bubble detaches from the surface for an even higher wettability condition (θ = 10°). Thus surface wettability and surface tension become two important factors governing the development of entrapped air film and bubble elimination in many practical applications.