bluuebunny/crossref_metadata_2025_split huggingface dataset:
Choroid plexus-selective inactivation of adenosine A2A receptors protects against T cell infiltration and experimental autoimmune encephalomyelitis
Authors: Zheng Wu , Feng Yijia , Zeng Zhenhai , Ye Mengqian , Wang Mengru , Liu Xin , Tang Ping , Shang Huiping , Sun Xiaoting , Lin Xiangxiang , Wang Muran , Li Zhengzheng , Weng Yiyun , Guo Wei , Vakal Sergii , Chen Jiang-fan 
Published: February 2022

Abstract
Background
Multiple sclerosis (MS) is one of the most common autoimmune disorders characterized by the infiltration of immune cells into the brain and demyelination. The unwanted immunosuppressive side effect of therapeutically successful natalizumab led us to focus on the choroid plexus (CP), a key site for the first wave of immune cell infiltration in experimental autoimmune encephalomyelitis (EAE), for the control of immune cells trafficking. Adenosine A2A receptor (A2AR) is emerging as a potential pharmacological target to control EAE pathogenesis. However, the cellular basis for the A2AR-mediated protection remains undetermined.

Methods
In the EAE model, we assessed A2AR expression and leukocyte trafficking determinants in the CP by immunohistochemistry and qPCR analyses. We determined the effect of the A2AR antagonist KW6002 treatment at days 8–12 or 8–14 post-immunization on T cell infiltration across the CP and EAE pathology. We determined the critical role of the CP-A2AR on T cell infiltration and EAE pathology by focal knock-down of CP-A2AR via intracerebroventricular injection of CRE-TAT recombinase into the A2ARflox/flox mice. In the cultured CP epithelium, we also evaluated the effect of overexpression of A2ARs or the A2AR agonist CGS21680 treatment on the CP permeability and lymphocytes migration.

Results
We found the specific upregulation of A2AR in the CP associated with enhanced CP gateway activity peaked at day 12 post-immunization in EAE mice. Furthermore, the KW6002 treatment at days 8–12 or 8–14 post-immunization reduced T cell trafficking across the CP and attenuated EAE pathology. Importantly, focal CP-A2AR knock-down attenuated the pathogenic infiltration of Th17+ cells across the CP via inhibiting the CCR6–CCL20 axis through NFκB/STAT3 pathway and protected against EAE pathology. Lastly, activation of A2AR in the cultured epithelium by A2AR overexpression or CGS21680 treatment increased the permeability of the CP epithelium and facilitated lymphocytes migration.

Conclusion
These findings define the CP niche as one of the primary sites of A2AR action, whereby A2AR antagonists confer protection against EAE pathology. Thus, pharmacological targeting of the CP-A2AR represents a novel therapeutic strategy for MS by controlling immune cell trafficking across CP.
Repeated endo-tracheal tube disconnection generates pulmonary edema in a model of volume overload: an experimental study
Authors: Katira Bhushan H. , Engelberts Doreen , Bouch Sheena , Fliss Jordan , Bastia Luca , Osada Kohei , Connelly Kim A. , Amato Marcelo B. P. , Ferguson Niall D. , Kuebler Wolfgang M. , Kavanagh Brian P. , Brochard Laurent J. , Post Martin 
Published: February 2022

Abstract
Background
An abrupt lung deflation in rodents results in lung injury through vascular mechanisms. Ventilator disconnections during endo-tracheal suctioning in humans often cause cardio-respiratory instability. Whether repeated disconnections or lung deflations cause lung injury or oedema is not known and was tested here in a porcine large animal model.

Methods
Yorkshire pigs (~ 12 weeks) were studied in three series. First, we compared PEEP abruptly deflated from 26 cmH2O or from PEEP 5 cmH2O to zero. Second, pigs were randomly crossed over to receive rapid versus gradual PEEP removal from 20 cmH2O. Third, pigs with relative volume overload, were ventilated with PEEP 15 cmH2O and randomized to repeated ETT disconnections (15 s every 15 min) or no disconnection for 3 h. Hemodynamics, pulmonary variables were monitored, and lung histology and bronchoalveolar lavage studied.

Results
As compared to PEEP 5 cmH2O, abrupt deflation from PEEP 26 cmH2O increased PVR, lowered oxygenation, and increased lung wet-to-dry ratio. From PEEP 20 cmH2O, gradual versus abrupt deflation mitigated the changes in oxygenation and vascular resistance. From PEEP 15, repeated disconnections in presence of fluid loading led to reduced compliance, lower oxygenation, higher pulmonary artery pressure, higher lung wet-to-dry ratio, higher lung injury score and increased oedema on morphometry, compared to no disconnects.

Conclusion
Single abrupt deflation from high PEEP, and repeated short deflations from moderate PEEP cause pulmonary oedema, impaired oxygenation, and increased PVR, in this large animal model, thus replicating our previous finding from rodents. Rapid deflation may thus be a clinically relevant cause of impaired lung function, which may be attenuated by gradual pressure release.
Healthcare-associated infections and antimicrobial use in Belgian nursing homes: results of three point prevalence surveys between 2010 and 2016
Authors: Latour Katrien , Catry Boudewijn , Devleesschauwer Brecht , Buntinx Frank , De Lepeleire Jan , Jans Béatrice 
Published: February 2022

Abstract
Background
Belgium monitors the burden of healthcare-associated infections (HAIs) and antimicrobial use in nursing homes (NHs) by participating in the European point prevalence surveys (PPSs) organised in long-term care facilities (HALT surveys). We present the main findings of the three national PPSs conducted in NHs participating in at least one of these surveys, and in a cohort that participated in all three consecutive surveys.

Methods
All NHs were invited to voluntarily participate and conduct the survey on one single day in May-September 2010 (HALT-1), in April-May 2013 (HALT-2) or in September-November 2016 (HALT-3). Data were collected at institutional, ward and resident level. A detailed questionnaire had to be completed for all eligible (i.e. living full time in the facility since at least 24 h, present at 8:00 am and willing to participate) residents receiving at least one systemic antimicrobial agent and/or presenting at least one active HAI on the PPS day. The onset of signs/symptoms had to occur more than 48 h after the resident was (re-)admitted to the NH.

Results
A total of 107, 87 and 158 NHs conducted the HALT-1, HALT-2 and HALT-3 survey, respectively. The median prevalence of residents with antimicrobial agent(s) increased from 4.3% (95% confidence interval (CI): 3.5-4.8%) in HALT-1 to 4.7% (95% CI: 3.5-6.5%) in HALT-2 and 5.0% (95% CI: 4.2-5.9%) in HALT-3. The median prevalence of residents with HAI(s) varied from 1.8% (95% CI: 1.4-2.7%) in HALT-1 to 3.2% (95% CI: 2.2-4.2%) in HALT-2 and 2.7% (95% CI: 2.1-3.4%) in HALT-3. Our post-hoc analysis on the cohort (n = 25 NHs) found similar trends. In all three surveys, respiratory tract infections were most frequently reported, followed by skin/wound infections in HALT-1 and urinary tract infections in HALT-2 and HALT-3. Antimicrobials were most commonly prescribed for the therapeutic treatment of an infection: 66.4% in HALT-1, 60.9% in HALT-2 and 64.1% in HALT-3. Uroprophylaxis accounted for 28.7%, 35.6% and 28.4% of all prescriptions, respectively.

Conclusions
None withstanding the limitations peculiar to the study design, the PPSs enabled us to assess the occurrence of and to increase awareness for HAIs and rational antimicrobial use in NHs at both local and national level.
Development and validation of contextual measures of sexual harassment perceptions, experiences, and coping for women employees in Ethiopian hospitality workplaces
Authors: Worke Mulugeta Dile , Koricha Zewdie Birhanu , Debelew Gurmesa Tura 
Published: February 2022

Abstract
Background
Sexual harassment among female employees in the hospitality industry is a complex phenomenon, and it has ramifications for employment, psychological, physical, and reproductive health. Nevertheless, our interpretation is constrained by a lack of agreement on its definition and measurement. As a result, hospitality workplaces require accurate tools that provide a detailed understanding of sexual harassment and inputs for action to limit adverse outcomes. Thus, this study aimed to develop a reliable and valid measure of female hospitality employees’ perceptions, experiences, and coping features concerning sexual harassment.

Methods
Item development, scale development, and scale evaluation were all parts of the design process. Following a round of feedback from the expert group, qualitative results, and a comprehensive literature review on related themes, item pools were created for the first version of the questionnaire. Pre-testing, survey administration, item reduction, and transformation of extracted latent factors of individual items into a unified and measurable construct were also performed. Field testing included five cognitive interviews with women who had experienced sexual harassment, a pre-test study of 30 women, and a survey of 345 women who worked in hospitality workplaces. Finally, tests for dimensionality, reliability, and validity were conducted.

Results
In Bahir Dar, Ethiopia, 345 women working in the hospitality workplaces, with a mean age of 24.31 ± 4.30 years, took part in this study. The robust maximum likelihood estimation approach was used to do confirmatory factor analysis. The model’s stability was determined by calculating the goodness of fit and the factorial invariance. Subsequently, the validity was confirmed. The findings supported the validity and reliability of the questionnaires designed for the target group. Therefore, the questionnaires can be used as research instruments.

Conclusions
The sexual harassment perceptions, experiences and coping scales provide a locally verified method for assessing sexual harassment in Ethiopia by government authorities and local and international non-governmental organisations, which aid in providing necessary services and the evaluation of efforts aimed at improving workplace safety, workplace health, and psychosocial well-being.
The financial feasibility of compressed biomethane gas application in Thailand
Authors: Tonrangklang Pennapa , Therdyothin Apichit , Preechawuttipong Itthichai 
Published: February 2022

Abstract
Background
Biomethane is a clean fuel upgraded from biogas. It has similar properties to natural gas vehicle (NGV) and can be used as a substitute for fossil fuels in the transportation and industrial sectors. Currently, biomethane is not in widespread use as alternative energy because it depends on local biogas production. However, there are few replacement alternatives and the commercial system is only on a small scale. Therefore, the overall aim of this paper is to study the financial feasibility of local biogas aggregation for producing biomethane on various scales. The results of the study are expected to aid the decision-making of investors and governments in promoting the use of renewable and sustainable sources of energy.

Methods
Primary and secondary data are used in this research for descriptive and quantitative analysis, while net present value, internal rate of return, payback period, and cost per unit represent the financial instruments. The location and distance methods applied are Google Maps and Power BI software programs, based on biogas upgrading by water scrubbing.

Results
The results indicate that 10 potential biomethane production areas exist in Thailand, with a total biogas production capacity of 6,000 m3/day or more under gas pipeline conditions not exceeding 50 km. Compressed biomethane gas plants with a capacity of less than 6 tons/day should be funded by the government at 30% of the total investment for a payback period of 5–6 years. Plants producing more than 6 tons/day provide a good return on investment even without government funding.

Conclusions
While this study focuses solely on areas of Thailand, it is the researcher’s position that the integration of residual biogas plants provides greater benefit than flare, particularly in remote areas. The model proposed in this study can serve as a prototype for developing countries such as Malaysia, the Philippines, Latin America, etc. Furthermore, it can be used in developed countries committed to reducing greenhouse gas emissions in accordance with the Paris Agreement.
Evidence based disease control methods in potato production: a systematic map protocol
Authors: Vilvert Elisa , Stridh Linnea , Andersson Björn , Olson Åke , Aldén Louise , Berlin Anna 
Published: February 2022

Abstract
Background
Several challenges, e.g. global trade, population growth, and climate change create future challenges for food production and food safety. In order to meet this, we need to secure and increase agricultural production with minimal environmental impact. Potato (Solanum tuberosum) ranks as one of the world’s most important crops for human consumption. While potato production and consumption have decreased in Europe and North America, global production has grown in the last decades due to the expansion of potato consumption in Asia. Potato is vulnerable to a wide range of pathogenic organisms, all of which can cause severe quality and yield losses. As a consequence, potato production is highly reliant on pesticide use, and this has a negative effect on the sustainability of the crop. To mitigate these problems, effective and evidence based crop protection recommendations need to be provided to growers.

Methods and output
The overarching aim of this project is to support the development of better methods of integrated pest management (IPM), as well as to identify alternative control methods for potato diseases to contribute to effective plant protection solutions and a more sustainable potato production. The specific objective of this systematic map is to provide a worldwide overview of plant disease protection measures available for potato production. All methods to control diseases within different cropping systems will be considered, such as pesticide application, biological control methods, resistant cultivars as well as disease support systems and tools for diagnosis. The systematic map will be presented as a searchable database where the volume and main characteristics of the relevant scientific literature will be described. We will identify evidence clusters and knowledge gaps in potato disease management and identify future research areas, and in this way contribute to new and innovative solutions. The map will provide important information and support for researchers and stakeholders, in particular authorities and advisory organizations. It will also help to select topics for future systematic reviews and meta-studies within potato research.
Addition of Navitoclax to Ongoing Ruxolitinib Therapy for Patients With Myelofibrosis With Progression or Suboptimal Response: Phase II Safety and Efficacy
Authors: Harrison Claire N. , Garcia Jacqueline S. , Somervaille Tim C.P. , Foran James M. , Verstovsek Srdan , Jamieson Catriona , Mesa Ruben , Ritchie Ellen K. , Tantravahi Srinivas K. , Vachhani Pankit , O'Connell Casey L. , Komrokji Rami S. , Harb Jason , Hutti Jessica E. , Holes Leanne , Masud Abdullah A. , Nuthalapati Silpa , Potluri Jalaja , Pemmaraju Naveen 
Published: February 2022

PURPOSE Targeting the BCL-XL pathway has demonstrated the ability to overcome Janus kinase inhibitor resistance in preclinical models. This phase II trial investigated the efficacy and safety of adding BCL-XL/BCL-2 inhibitor navitoclax to ruxolitinib therapy in patients with myelofibrosis with progression or suboptimal response to ruxolitinib monotherapy (ClinicalTrials.gov identifier: NCT03222609 ). METHODS Thirty-four adult patients with intermediate-/high-risk myelofibrosis who had progression or suboptimal response on stable ruxolitinib dose (≥ 10 mg twice daily) were administered navitoclax at 50 mg once daily starting dose, followed by escalation to a maximum of 300 mg once daily in once in weekly increments (if platelets were ≥ 75 × 109/L). The primary end point was ≥ 35% spleen volume reduction (SVR35) from baseline at week 24. Secondary end points included ≥ 50% reduction in total symptom score (TSS50) from baseline at week 24, hemoglobin improvement, change in bone marrow fibrosis (BMF) grade, and safety. RESULTS High molecular risk mutations were identified in 58% of patients, and 52% harbored ≥ 3 mutations. SVR35 was achieved by 26.5% of patients at week 24, and by 41%, at any time on study, with an estimated median duration of SVR35 of 13.8 months. TSS50 was achieved by 30% (6 of 20) of patients at week 24, and BMF improved by 1-2 grades in 33% (11 of 33) of evaluable patients. Anemia response was achieved by 64% (7 of 11), including one patient with baseline transfusion dependence. Median overall survival was not reached with a median follow-up of 21.6 months. The most common adverse event was reversible thrombocytopenia without clinically significant bleeding (88%). CONCLUSION The addition of navitoclax to ruxolitinib in patients with persistent or progressive myelofibrosis resulted in durable SVR35, improved TSS, hemoglobin response, and BMF. Further investigation is underway to qualify the potential for disease modification.
Liquid demixing in elastic networks: Cavitation, permeation, or size selection?
Authors: Ronceray Pierre , Mao Sheng , Košmrlj Andrej , Haataja Mikko P. 
Published: February 2022

Abstract
In cells, phase-separated liquid condensates interact mechanically with surrounding elastic networks such as chromatin and cytoskeleton. By considering the trade-offs between elastic, wetting, and interfacial energies, we theoretically show that three droplet phases can be thermodynamically stable: macroscopic droplets that either cavitate or permeate the network, and mesh-size–limited microdroplets. We show that network strain stiffening further enhances this latter size-limitation effect. Our theory predicts the possibility of yet-unobserved droplet phases in the cytoplasm and nucleoplasm.
Scalar perturbation of gravitating double-kink solutions
Authors: Feng Jun , Zhong Yuan 
Published: February 2022

Abstract
In this letter, a two-dimensional (2D) gravity-scalar model is studied. This model supports interesting double-kink solutions, and the corresponding metric solutions can be derived analytically. Depending on a tunable parameter c, the metric can be symmetric or asymmetric. The Schrödinger-like equation for normal modes of the physical linear perturbation is derived. As c varies, the effective potential can have one or two singular barriers. If c is larger than a critical value, the zero mode will be normalizable, despite of the appearance of a strong repulsive singularity. The double-kink solution is always stable against linear perturbations.
The Practice of Pediatric Cardiac Anesthesiology in the United States
Authors: Nasr Viviane G. , Staffa Steven J. , Vener David F. , Huang ShengXiang , Brown Morgan L. , Twite Mark , Miller-Hance Wanda C. , DiNardo James A. 
Published: February 2022

BACKGROUND:
With advances in surgical and catheter-based interventions and technologies in patients with congenital heart disease (CHD), the practice of pediatric cardiac anesthesiology has evolved in parallel with pediatric cardiac surgery and pediatric cardiology as a distinct subspecialty over the past 80 years. To date, there has not been an analysis of the distribution of pediatric cardiac anesthesiologists relative to cardiac and noncardiac procedures in the pediatric population. The primary aim is to report the results of a survey and its subsequent analysis to describe the distribution of pediatric cardiac anesthesiologists relative to pediatric cardiac procedures that include surgical interventions, cardiac catheterization procedures, imaging studies (echocardiography, magnetic resonance, computed tomography, positron emission tomography), and noncardiac procedures.

METHODS:
A survey developed in Research Electronic Data Capture (REDcap) was sent to the identifiable division chiefs/cardiac directors of 113 pediatric cardiac anesthesia programs in the United States. Data regarding cardiac surgical patients and procedures were collected from the Society of Thoracic Surgeons Congenital Heart Surgery Database (STS-CHD).

RESULTS:
This analysis reveals that only 38% (117 of 307) of pediatric cardiac anesthesiologists caring for patients with CHD pursued additional training in pediatric cardiac anesthesiology, while 44% (136 of 307) have gained experience during their clinical practice. Other providers have pursued different training pathways such as adult cardiac anesthesiology or pediatric critical care. Based on this survey, pediatric cardiac anesthesiologists devote 35% (interquartile range [IQR], 20%–50%) of clinical time to the care of patients in the cardiac operating room, 25% (20%–35%) of time to the care of patients in the cardiac catheterization laboratory, 10% (5%–10%) to patient care in imaging locations, and 15% covering general pediatric, adult, or cardiac patients undergoing noncardiac procedures. Attempts to actively recruit pediatric cardiac anesthesiologists were reported by 49.2% (29 of 59) of the institutions surveyed. Impending retirement of staff was anticipated in 17% (10 of 59) of the institutions, while loss of staff to relocation was anticipated in 3.4% (2 of 59) of institutions. Thirty-seven percent of institutions reported that they anticipated no immediate changes in current staffing levels.

CONCLUSIONS:
The majority of currently practicing pediatric cardiac anesthesiologists have not completed a fellowship training in the subspecialty. There is, and will continue to be, a need for subspecialty training to meet increasing demand for services especially with increase survival of this patient population and to replace retiring members of the workforce.