bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of ethylene oxide, gamma radiation, dry heat and autoclave sterilization processes on extracellular matrix of biomaterial dental scaffolds
Authors: de Sousa Iwamoto Luciana Aparecida , Duailibi Mônica Talarico , Iwamoto Gerson Yoshinobu , de Oliveira Débora Cristina , Duailibi Silvio Eduardo 
Published: March 2022

AbstractScaffolds used to receive stem cells are a promising perspective of tissue regeneration research, and one of the most effective solutions to rebuild organs. In the near future will be possible to reconstruct a natural tooth using stems cells, but to avoid an immune-defensive response, sterilize the scaffold is not only desired, but also essential to be successful. A study confirmed stem cells extracted from rat’s natural teeth, and implanted into the alveolar bone, could differentiate themselves in dental cells, but the scaffold’s chemistry, geometry, density, morphology, adherence, biocompatibility and mechanical properties remained an issue. This study intended to produce a completely sterilized dental scaffold with preserved extracellular matrix. Fifty-one samples were collected, kept in formaldehyde, submitted to partial demineralization and decellularization processes and sterilized using four different methods: dry heating; autoclave; ethylene-oxide and gamma-radiation. They were characterized through optical images, micro-hardness, XRD, EDS, XRF, SEM, histology and sterility test. The results evidenced the four sterilization methods were fully effective with preservation of ECM molecular arrangements, variation on chemical composition (proportion of Ca/P) was compatible with Ca/P proportional variation between enamel and dentine regions. Gamma irradiation and ethylene oxide presents excellent results, but their viability are compromised by the costs and technology’s accessibility (requires very expensive equipment and/or consumables). Excepted gamma irradiation, all the sterilization methods more than sterilizing also reduced the remaining pulp. Autoclave presents easy equipment accessibility, lower cost consumables, higher reduction of remaining pulp and complete sterilization, reason why was considered the most promising technique.
Plasma-assisted three-dimensional lightscribe graphene as high-performance supercapacitors
Authors: Namdar Naser , Ghasemi Foad , Sanaee Zeinab 
Published: March 2022

AbstractGraphene-based supercapacitors demonstrate extraordinary energy storage capacity due to their layered structure, large effective surface area, high electrical conductivity and acceptable chemical stability. Herein, reduced graphene oxide (rGO)-based supercapacitors were introduced in a simple, and fast method with considerable performance. For this purpose, graphene oxide (GO) was synthesized by the modified Hummers’ method and then easily reduced to desired patterns of rGO using a commercial LightScribe DVD drive. In order to increase the effective surface area, as well as the electrical conductivity of rGO layers, oxygen/sulfur hexafluoride plasma was applied to the rGO followed by laser irradiation. By performing such sequential processes, an rGO-based supercapacitor was introduced with a capacitance of about 10.2 F/cm3, which had high stability for more than 1000 consecutive charge–discharge cycles. The fabrication steps of the electrodes were investigated by different analyses such as SEM, TEM, Raman, surface resistance, BET, and XPS measurements. Results showed that these rGO-based electrodes fabricated by sequential processes are very interesting for practical applications of energy storage.
Accuracy of B-mode ultrasound and ARFI elastography in predicting malignancy of canine splenic lesions
Authors: Maronezi Marjury Cristina , Carneiro Rafael Kretzer , da Cruz Igor Cezar Kniphoff , de Oliveira Ana Paula Luiz , De Nardi Andrigo Barboza , Pavan Letícia , Del’Aguila-Silva Priscila , Uscategui Ricardo Andrés Ramirez , Feliciano Marcus Antônio Rossi 
Published: March 2022

AbstractThe objective was to evaluate the accuracy of B-mode ultrasonography and ARFI elastography in detecting malignancy in canine splenic lesions. Thirty-seven spleens with abnormalities (16 benign and 21 malignant) from dogs of different breeds and ages were evaluated. Echogenicity, echotexture, organ length and height were evaluated using B-mode. By ARFI elastography, tissue stiffness was evaluated qualitatively (elastogram) and quantitatively (measuring the shear wave velocity—SWV). Lesions were classified as diffuse, focal or multifocal (cranial, medial or caudal portion) and comparisons of the SWV between the injured and non-injured areas were performed. In the B-mode, no features were associated to malignancy (P > 0.05). In the elastogram, 35 spleens were non-deformable and 2 deformable, having no association with malignancy. The greater SWV was observed in malignant lesions (3.4 ± 0.6 m/s), followed by areas free from alterations (2.1 ± 0.3 m/s) and benign lesions (1.7 ± 0.5 m/s), with difference between groups (P < 0.0001). It was found that a SWV > 2.6 m/s indicates malignancy of canine splenic lesions (sensitivity of 95%, specificity of 100%, PPV of 100%, NPV of 94% and accuracy of 97%), concluding that ARFI elastography is a promising technique for differentiating malignancy in these lesions.
Spectroscopic analysis reveals that soil phosphorus availability and plant allocation strategies impact feedstock quality of nutrient-limited switchgrass
Authors: Hao Zhao , Wang Yuan , Ding Na , Saha Malay C. , Scheible Wolf-Rüdiger , Craven Kelly , Udvardi Michael , Nico Peter S. , Firestone Mary K. , Brodie Eoin L. 
Published: March 2022

AbstractThe perennial native switchgrass adapts better than other plant species do to marginal soils with low plant-available nutrients, including those with low phosphorus (P) content. Switchgrass roots and their associated microorganisms can alter the pools of available P throughout the whole soil profile making predictions of P availability in situ challenging. Plant P homeostasis makes monitoring of P limitation via measurements of plant P content alone difficult to interpret. To address these challenges, we developed a machine-learning model trained with high accuracy using the leaf tissue chemical profile, rather than P content. By applying this learned model in field trials across two sites with contrasting extractable soil P, we observed that actual plant available P in soil was more similar than expected, suggesting that adaptations occurred to alleviate the apparent P constraint. These adaptations come at a metabolic cost to the plant that have consequences for feedstock chemical components and quality. We observed that other biochemical signatures of P limitation, such as decreased cellulose-to-lignin ratios, were apparent, indicating re-allocation of carbon resources may have contributed to increased P acquisition. Plant P allocation strategies also differed across sites, and these differences were correlated with the subsequent year’s biomass yields.
Improving the performance of practical decoy-state quantum key distribution with advantage distillation technology
Authors: Li Hong-Wei , Zhang Chun-Mei , Jiang Mu-Sheng , Cai Qing-Yu 
Published: March 2022

AbstractQuantum key distribution (QKD) provides a promising solution for sharing information-theoretic secret keys between two remote legitimate parties. To improve the maximal transmission distance and the maximal error rate tolerance, we apply the advantage distillation technology to analyze the security of practical decoy-state QKD systems. Based on the practical experimental parameters, the device-dependent QKD protocols and the measurement-device-independent QKD protocols have been respectively analyzed, and our analysis results demonstrate that the advantage distillation technology can significantly improve the performance of various QKD protocols. In the four-state and six-state device-dependent QKD protocols, we prove that the maximal transmission distance can be improved from 142 km to 180 km and from 146 km to 187 km respectively. In the four-state and six-state measurement-device-independent QKD protocols, we prove that the maximal transmission distance can be improved from 195 km to 273 km and from 200 km to 282 km respectively.
Mesocrystalline effect in a NiTiO3/TiO2 nanocomposite for enhanced capacity of lithium-ion battery anodes
Authors: Wang Xing , Cheng Weijie , Hu Jiaqiao , Su Ying , Kong Xingang , Uemura Shinobi , Kusunose Takafumi , Feng Qi 
Published: March 2022

Two kinds of NiTiO3/TiO2 nanocomposites can be synthesized from the layered HTO precursor. One is mesocrystalline NiTiO3/TiO2 nanocomposite from Ni-H2O2-HTO. Another is polycrystalline NiTiO3/TiO2 nanocomposite from Ni-H2O2-HTO-ns.
Spectrum of DNA mismatch repair failures viewed through the lens of cancer genomics and implications for therapy
Authors: Mas-Ponte David , McCullough Marcel , Supek Fran 
Published: March 2022

Abstract
Genome sequencing can be used to detect DNA repair failures in tumors and learn about underlying mechanisms. Here, we synthesize findings from genomic studies that examined deficiencies of the DNA mismatch repair (MMR) pathway. The impairment of MMR results in genome-wide hypermutation and in the ‘microsatellite instability’ (MSI) phenotype—occurrence of indel mutations at short tandem repeat (microsatellite) loci. The MSI status of tumors was traditionally assessed by molecular testing of a selected set of MS loci or by measuring MMR protein expression levels. Today, genomic data can provide a more complete picture of the consequences on genomic instability. Multiple computational studies examined somatic mutation distributions that result from failed DNA repair pathways in tumors. These include analyzing the commonly studied trinucleotide mutational spectra of single-nucleotide variants (SNVs), as well as of other features such as indels, structural variants, mutation clusters and regional mutation rate redistribution. The identified mutation patterns can be used to rigorously measure prevalence of MMR failures across cancer types, and potentially to subcategorize the MMR deficiencies. Diverse data sources, genomic and pre-genomic, from human and from experimental models, suggest there are different ways in which MMR can fail, and/or that the cell-type or genetic background may result in different types of MMR mutational patterns. The spectrum of MMR failures may direct cancer evolution, generating particular sets of driver mutations. Moreover, MMR affects outcomes of therapy by DNA damaging drugs, antimetabolites, nonsense-mediated mRNA decay (NMD) inhibitors, and immunotherapy by promoting either resistance or sensitivity, depending on the type of therapy.
Soft open points based load restoration for the urban integrated energy system under extreme weather events
Authors: Wang Xinyue , Li Xue , Li Xiaojing , Parisio Alessandra , Wang Changrong , Jiang Tao 
Published: March 2022

AbstractWith frequent occurrences of extreme natural disasters such as typhoons in urban integrated energy systems (UIES), it is of great significance to cope with different kinds of disturbances. This paper proposes a load restoration method under typhoon weather for urban distribution and natural gas networks based on soft open points (SOP). Firstly, the typhoon wind speed model is introduced and the line fault rates of distribution networks under typhoons are calculated. Secondly, the gas turbine and electric‐driven compressor are considered as the coupling units of the integrated electric–gas energy system, and related models are constructed. The fault analysis method of the natural gas network is proposed considering the faults in the distribution network lead by typhoons. Thirdly, SOP installed in the distribution network with the V/f control mode is applied to restore electrical loads and provide voltage support for the loads on the fault side. After that, the loads of the gas network could also be restored because of the restoration of the coupling units. Optimal energy flow is applied to determine the output of the power and gas sources, coupling units and also the loads to be restored. Finally, the fault rate of each line under typhoon disaster is analysed and the correctness and effectiveness of the resilience improving method based on SOP are verified with test systems UIES E33‐G14 and UIES E123‐G48.
Serum CYR61 Levels are Associated with Graves’ Ophthalmopathy
and Smoking in Patients with Graves’ Disease
Authors: Cerri Perparim , Shahida Bushra , Lantz Mikael , Planck Tereza 
Published: March 2022

AbstractSmoking is a well-known risk factor for Graves’ ophthalmopathy (GO) in
patients suffering from Graves’ disease (GD). Cysteine-rich angiogenic
inducer 61 (CYR61), which has multiple physiological functions, has been shown
to be associated with GD and GO. In this study, we aimed to investigate the
association between smoking and CYR61 concentrations in GD patients with and
without GO. Serum CYR61 was measured by ELISA. The association between CYR61
concentration and GO was assessed with binary logistic regression in all
patients and in subgroups of smokers and nonsmokers. The Spearman correlation
coefficient was used to determine the correlations between CYR61 concentration
and clinical parameters. CYR61 levels were significantly higher in GD patients
with GO than in patients without GO, in smokers than in nonsmokers and in
individuals older than 50 years than in those younger than 50 years. The
subgroup of “GO smokers” had the highest CYR61 levels [median
(IQR), 119 pg/ml (129.8)], compared with “GO
nonsmokers” [84.2 pg/ml (90.8), p=0.04],
“no GO smokers” [88.9 pg/ml (109.8),
p=0.01] and “no GO nonsmokers”
[79.4 pg/ml (129.89), p=0.003]. For each unit increase
in CYR61 concentration, the odds of having GO in smokers significantly and
independently increased by 1% (OR=1.010; 95% CI:
1.002–1.018, p=0.012). In conclusion, our results indicate that
smoking and age increase serum CYR61 levels in patients with GD and GO. The role
of CYR61 as a predictor of GO in patients with GD should be evaluated in
prospective studies.
Prognostic Value of Long Noncoding RNA SNHG11 in Patients with
Prostate Cancer
Authors: Li Ying , Pan Bin , Guo Xiuqin , Meng Xiaomin , Tian Xiaojing 
Published: March 2022

AbstractThe present study was aimed to explore the prognostic value of long noncoding RNA
SNHG11 in prostate cancer, study its expression, and assess its effect on tumor
progression. One hundred and twenty prostate cancer patients and 45 cases of
benign prostate hyperplasia (BPH) patients were collected. RT-qPCR was used to
test the expression of SNHG11 in prostate cancer and BPH tissues, as well as in
cell lines. Kaplan-Meier survival analysis and Cox regression assays were
introduced to evaluate the prognostic meaning of SNHG11 in prostate cancer. The
CCK-8 assays were performed to explore the effect of SNHG11 on prostate cancer
cell proliferation, and a Transwell assay was conducted to access the influence
of SNHG11 on prostate cancer cell migration and invasion. SNHG11 expression
level was upregulated both in prostate cancer tissues and cell lines.
Overexpression of SNHG11 was significantly associated with Gleason score,
clinical T stage, surgical margin status, and lymph node metastasis. Patients
with high SNHG11 expression levels led to a shorter overall survival time and
biochemical recurrence-free survival when compared with those of low expression
levels. Multivariate Cox regression results suggested that SNHG11 has the
potential to act as a prognostic marker for prostate cancer patients. Knockdown
of SNHG11 suppressed 22RV1 cell proliferation, migration, and invasion. In
conclusion, SNHG11 is upregulated in prostate cancer patients and predicts an
unfavorable prognosis for prostate cancer patients. Its knockdown can weaken
prostate cancer cell metastasis and growth in vitro.
Anti-Mullerian Hormone Levels Increase After Bariatric Surgery in
Obese Female Patients With and Without Polycystic Ovary Syndrome
Authors: Buyukkaba Mitat , Turgut Seda , Ilhan Mahmut Muzaffer , Ekinci Iskender , Yaylım İlhan , Zeybek Sakir Umit , Turan Saime , Tasan Ertugrul , Karaman Ozcan 
Published: March 2022

AbstractThis study was aimed to investigate the effect of weight loss by bariatric
surgery on the level of anti-Mullerian hormone (AMH) in morbidly obese female
patients with or without polycystic ovary syndrome (PCOS). This prospective
study includes 70 females, obese, and fertile patients of reproductive age. All
patients were evaluated to determine the changes in weight, body mass index
(BMI), serum AMH, and other biochemical parameters at the end of six months. The
mean levels of the preop and postop AMH were
1.66±0.87 ng/ml and
5.99±1.39 ng/ml in the PCOS group;
1.35±0.76 ng/ml and
6.23±1.47 ng/ml in the non-PCOS group, respectively. The
postop AMH levels were significantly higher than the preop levels for both
groups (p<0.001). There were significant differences in the level of
glucose, low-density lipoprotein (LDL), high-density lipoprotein (HDL),
triglyceride, total cholesterol, hemoglobin A1c, HOMA-IR, insulin between preop
and postop 6th month. A negative correlation was found between postop AMH and
body weight in all patients (r=–0.337, p=0.031). Postop
AMH levels were negatively correlated with postop BMI levels in the non-PCOS
patient group (r=–0.408, p=0.043). No significant
difference was observed between the PCOS and non-PCOS groups in terms of all the
parameters examined. In conclusion, our study suggests that the significantly
increased AMH levels by losing weight with bariatric surgery in patients with
morbid obesity with and without PCOS may indicate the improvement of
fertilization potential. It could be considered when evaluating fertility in
patients with morbid obesity.
Chiral Ammonium Salt Catalyzed Asymmetric Alkylation of Unactivated Amides
Authors: Xu Changming , Yang Xinshuang 
Published: March 2022

AbstractA chiral spirocyclic quaternary ammonium salt catalyzed asymmetric alkylation of unactivated amides under homogeneous conditions has been developed, giving the target products with up to 60% ee. This novel methodology provides a general and concise protocol for asymmetric α-functionalization of unactivated amides.
Microwave-Assisted Hydrogen-Free Reductive Deiodination of Iodoarenes with Silicon-Nanoarray Palladium-Nanoparticle Catalyst
Authors: Yamada Yoichi M. A. , Matsukawa Yuta 
Published: March 2022

AbstractThe hydrogenolysis of iodoarenes using a silicon nanoarray palladium catalyst under microwave irradiation was investigated. When triethanolamine was used as the sacrificial reductant, the reaction proceeded via reductive deiodination under an aerobic atmosphere even without the presence of the explosive hydrogen gas, affording the corresponding hydrogen-substituted arenes in high yields. No reaction occurred in the absence of microwaves, indicating a noticeable microwave effect.
Correlação entre posicionamento do implante das artroplastias parciais de recobrimento do ombro e os resultados funcionais
Authors: Luz Gilberto Daniel , Cavalcanti Amanda S. , Ferreira Júlio , Godoy Eduardo , Amaral Marcus Vinicius Galvão , Motta Filho Geraldo da R. 
Published: March 2022

Resumo
Objetivo O objetivo do presente estudo é correlacionar os resultados funcionais de uma série de casos de artroplastias parciais de recobrimento do ombro com o posicionamento do implante.
Métodos Um total de 25 pacientes foram avaliados em relação à amplitude de movimentos, à avaliação funcional pelo escore de Universidade da Califórnia Los Angeles (UCLA) e por análise radiográfica. Os dados pré- e pós-operatórios foram comparados. Adicionalmente, os pacientes foram agrupados quanto ao ângulo cérvico-diafisário (ACD) avaliado na radiografia em anteroposterior e quanto ao ângulo de retroversão (ARV) avaliado na radiografia em posição axilar. Foi considerado como posicionamento ideal (padrão anatômico) um ACD entre 130° e 140° e um ARV entre 20° e 40°. Os dados foram analisados pelo teste pareado de Wilcoxon, pela análise de variância (ANOVA, na sigla em inglês) seguida pelo pós-teste de Kruskal-Wallis ou pelo teste de Mann-Whitney, quando apropriado.
Resultados O seguimento médio foi de 48,3 meses (12 a 67 meses). A avaliação funcional pós-operatória (31,5) foi melhor do que a pré-operatória (15,5) (p < 0,001). Seis pacientes apresentaram posicionamento anatômico do implante, enquanto 19 pacientes foram considerados “fora do padrão.” Sete pacientes apresentaram um ACD < 130°, quatorze apresentaram um ACD entre 130° e 140°, e quatro apresentaram um ACD >140°. Quinze pacientes apresentaram um ARV ≤ 20°, e 10 entre 20° e 40°. Utilizando esses critérios para agrupar os pacientes, a comparação dos parâmetros da avaliação clínico-funcional pós-operatória não foi estatisticamente diferente (p > 0,05).
Conclusão A artroplastia parcial de recobrimento do ombro oferece significativa recuperação funcional pós-operatória em pacientes com doenças degenerativas articulares. Entretanto, o posicionamento do implante avaliado pelos ACD e ARV não se correlaciona com o resultado clínico-funcional, sendo, portanto, uma medida imprecisa de sucesso da cirurgia.Nível de Evidência IV, Série de Casos.
Estudo epidemiológico das lesões multiligamentares do joelho
Authors: Pinheiro Igor Natário , Pedrinha Igor Stefano Menescal , Maia Phelippe Augusto Valente , Cortes André Rodrigues de Oliveira , Albuquerque Rodrigo Pires e , Barretto João Maurício 
Published: March 2022

Resumo
Objetivo Descrever e associar as características das lesões multiligamentares de joelho com o perfil do paciente e mecanismo de trauma.
Métodos Trata-se de um estudo transversal que avaliou 82 pacientes com lesões multiligamentares do joelho de setembro de 2016 até setembro de 2018. As variáveis coletadas foram idade, gênero, eixo mecânico, lateralidade, arco de movimento, mecanismo do trauma, lesões associadas, ligamentos afetados e afastamento do trabalho.
Resultados A amostra incluiu pacientes de 16 a 58 anos, com média de 29,7 anos, e os homens foram os mais afetados, correspondendo a 92,7% dos casos. O mecanismo de trauma mais comum foi acidente motociclístico (45,1%). O ligamento mais lesado foi o ligamento cruzado anterior (80,5%), seguido do ligamento cruzado posterior (77,1%), do canto posterolateral (61,0%) e do ligamento colateral tibial (26,8%). O tipo de luxação mais frequente era o KD IIIL (30,4%). Apenas 1 paciente apresentou lesão vascular, e 13 (15,9%) apresentaram lesões neurológicas. A maioria das vítimas foi afastada do trabalho (52,4%).
Conclusão Há grande diferença entre os pacientes que apresentam lesão multiligamentar no Brasil em relação ao encontrado nos estudos internacionais. Desta forma, convém realizar mais estudos específicos sobre o tema com a nossa população, de modo a aperfeiçoar o tratamento destes pacientes.