bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and safety of belimumab in systemic lupus erythematosus patients with severe lupus nephritis requiring renal replacement therapy
Authors: Liu Diankun , Zhou Qiang , Wang Di , Qu Yuan , Guo Qihong , Wen Jing , Yu Qinghong , Ai Jun 
Published: August 2022

Background Systemic lupus erythematosus is a chronic inflammatory autoimmune disease that has various manifestations. Lupus nephritis is a common and severe presentation, which results in increased morbidity and mortality. Belimumab added on standard therapy has been proved to induce disease remission and improve renal parameters. However, the use of belimumab has not been explored in patients requiring dialysis treatment. Methods Seven patients diagnosed as SLE with renal involvement requiring dialysis, who received belimumab in addition to steroids or immunosuppressants were identified. Clinical and biological data were extracted from medical records and laboratory databases. Ten mg/kg belimumab was applied on day 1, 15, 29, and every 28 days thereafter for a total of 8 dose. Renal parameters including urine output and serum creatinine level, immunologic index including anti-ds-DNA antibody titer and complement level, and disease activity were documented to reveal the response to belimumab. Results After belimumab therapy, all the 7 patients receiving dialysis therapy showed immunologic improvement. Disease activity significantly declined from 16.5 to 5.33 using SLEDAI-2K score. Apart from patient 7 on maintenance dialysis, 5 of 6 patients had increased urine output and were out of dialysis treatment. Patient 5 and 6 showed significant decrease in serum creatinine level. Only one pulmonary infection was documented. Conclusions Belimumab added to steroids or immunosuppressive agents was able to improve renal and immunologic parameters and decrease disease activity of SLE patients receiving dialysis treatment. The safety issue is promising with no severe adverse effect recorded. Further large, controlled, randomized clinical trials are required to confirm the results.
Electroacupuncture relieves visceral hypersensitivity through modulation of the endogenous cannabinoid system
Authors: Ma Ning , Li Xiaojing , Li Qiuhua , Yang Diqi , Zhuang Shen , Nan Sha , Liu Ai , Ding Mingxing , Ding Yi 
Published: August 2022

Background: Electroacupuncture (EA) can effectively relieve visceral hypersensitivity (VH). However, its mechanisms are still unclear. Objective: To investigate the impact of EA on VH caused by ileitis, and whether EA relieves VH by modulating the endogenous cannabinoid system (ECS). Methods: Thirty male native goats were randomly divided into a saline-treated control group (Saline, n = 9) and three 2,4,6-trinitro-benzenesulfonic acid (TNBS)-treated VH model groups that underwent injection of TNBS into the ileal wall to induce VH and remained untreated (TNBS, n = 9) or received six sessions of EA (for 30 min every 3 days) (TNBS + EA, n = 6) or sham acupuncture (TNBS + Sham, n = 6). The visceromotor response (VMR) to colorectal distention (CRD) was measured after each EA treatment. Three goats in the Saline/TNBS groups were euthanized after 7 days for histopathological examination; the remaining 24 (n = 6/group) underwent sampling of the ileal wall, T11 spinal cord and brain nuclei/areas related to visceral regulation and ascending pain modulation system on day 22. Expression of cannabinoid receptor 1 (CB1R), fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) was detected by immunohistochemistry. Results: VMR to CRD was greater in TNBS-treated goats than in saline-treated goats (p < 0.01) from day 7 to 22. After day 7, EA-treated goats showed a decreased (p < 0.05) VMR compared with untreated TNBS-exposed goats. TNBS treatment decreased CB1R and increased FAAH and MAGL expression in the ileum and related nuclei/areas; this was reversed by EA. Conclusion: EA ameliorates VH, probably by regulating the ECS in the intestine and nuclei/areas related to visceral regulation and descending pain modulation systems.
Optic neuritis following COVID-19 vaccination: Do autoimmune diseases play a role?
Authors: Pirani Vittorio , Pelliccioni Paolo , Carpenè Maria Jolanda , Nicolai Michele , Barbotti Federica , Franceschi Alessandro , Mariotti Cesare 
Published: August 2022

Purpose and context We report two cases of optic neuritis following Pfizer-BioNTech COVID-19 vaccination in patients with autoimmune diseases. Post-vaccine optic neuritis, although rare, is reported in the literature and an autoimmune mechanism seems to be involved, especially in genetically predisposed subjects. Method Report of two cases of optic neuritis following COVID-19 vaccination in patients with autoimmune diseases. Key results He novelty of this article is the fact that the two patients had a medical history of autoimmune diseases (Hashimoto thyroiditis and ankylosing spondylitis), a point of interest for research because it is not yet clear whether this could constitute a risk factor for adverse events after vaccination. Conclusions The reactogenicity of COVID-19 mRNA vaccine in individuals suffering from immune-mediated diseases is yet to be elucidated and it is being increasingly investigated. Our paper supports the hypothesis that patients who are already affected or predisposed to autoimmune or autoinflammatory disorders should be carefully evaluated for the benefits and risks of COVID-19 mRNA vaccination.
Adverse Childhood Experiences and Obesity: A One-to-One Correlation?
Authors: Mahmood Sara , Li Yixuan , Hynes Marijane 
Published: August 2022

Introduction Adverse childhood experiences (ACEs) have been associated with physical and emotional problems such as obesity and depression in adulthood. This study evaluated the relationship between ACE scores and the severity of obesity. Methods We conducted a cross-sectional study of 119 adult patients at the GW Weight Management Clinic. They filled out an ACE survey examining trauma and household dysfunction. The main outcome was the severity of obesity measured by Body Mass Index (BMI). We estimated the adjusted mean difference between high and low ACE risk groups using - linear regression. Results The data suggest a positive relationship between ACE score and BMI. Patients with severe obesity are more likely to be a high ACE risk (50%) compared to others (24–25%). The average BMI in the high ACE risk group is 3.64 (95% CI: 0.25–7.03) higher than those in the low ACE risk group. The linear regression also showed that as the ACE score increased by 1, BMI increased by 1 unit (95% CI: 0.15–1.77). Conclusions Our findings suggest ACE is not just related with the prevalence of obesity, but also the severity of obesity in the overweight and obese population. The linear regression also showed that as the ACE score increased by 1, the BMI increased by 1 unit as well.
Mobile and online consumer tools to screen for autism do not promote equity
Authors: Sanders Benjamin W , Bedrick Steven , Broder-Fingert Sarabeth , Brown Shannon A , Dolata Jill K , Fombonne Eric , Reeder Julie A , Rivas Vazquez Luis Andres , Fuchu Plyce , Morales Yesenia , Zuckerman Katharine E 
Published: August 2022

Limited access to screening and evaluation for autism spectrum disorder in children is a major barrier to improving outcomes for marginalized families. To identify and evaluate available digital autism spectrum disorder screening resources, we simulated web and mobile app searches by a parent concerned about their child’s likelihood of autism spectrum disorder. Included digital autism spectrum disorder screening tools (a) were on Internet or mobile app; (b) were in English; (c) had a parent user inputting data; (d) assigned likelihood category to child <9 years; and (e) screened for autism spectrum disorder. Ten search terms, developed using Google Search and parent panel recommendations, were used to search web and app tools in the United States, the United Kingdom, India, Australia, and Canada using Virtual Private Networks. Results were examined for attributes likely to benefit parents in marginalized communities, such as ease of searching, language versions, and reading level. The four terms most likely to identify any tools were “autism quiz,” “autism screening tool,” “does my child have autism,” and “autism toddler.” Three out of five searches contained autism spectrum disorder screening tools, as did one of 10 links or apps. Searches identified a total of 1475 websites and 919 apps, which yielded 23 unique tools. Most tools required continuous Internet access or offered only English, and many had high reading levels. In conclusion, screening tools are available, but they are not easily found. Barriers include inaccessibility to parents with limited literacy or limited English proficiency, and frequent encounters with games, advertisements, and user fees. Lay Abstract Many parents wonder if their child might have autism. Many parents use their smartphones to answer health questions. We asked, “How easy or hard is it for parents to use their smartphones to find ‘tools’ to test their child for signs of autism?” After doing pretend parent searches, we found that only one in 10 search results were tools to test children for autism. These tools were not designed for parents who have low income or other challenges such as low literacy skills, low English proficiency, or not being tech-savvy.
The Influence of Different γ-Irradiation Patterns on Factors that May Affect Cell Cycle Progression in Male Rats
Authors: Mohammed Manal R. , El-Bahkery Azza M. , Shedid Shereen M. 
Published: August 2022

Most studies of the biological effects of ionizing radiation have been done on a single acute dose, while clinically and environmentally exposures occur under chronic/repetitive conditions. It is important to study effects of different patterns of ionizing radiation. In this study, a rat model was used to compare the effects of repetitive and acute exposure. Groups: (I) control, (II, III) were exposed to fractionated doses (1.5 GyX4) and (2 GyX4), respectively/24h interval, and (IV, V) were exposed to 6 Gy and 8 Gy of whole-body gamma irradiation, respectively. The gene expression of MAPT and tau phosphorylation increased in all irradiated groups but the gene expression of PKN not affected. TGFβ% increased at dose of 2 GyX4 only. In addition, the cell cycle was arrested in S phase. Micronucleus (MN) increased and cell proliferation decreased. In conclusion, the dose and pattern of ionizing radiation do not affect the MAPT and PKN gene expression, but TGF-β, p-tau, MN assay and cell proliferation are significantly affected. The dose of 2 GyX4 showed distinctive effect. Repetitive exposure may increase TGF-β%, which causes radio-resistance and, G2/M delay. Thus, the cell cycle could be regulated in a different manner according to the dose and pattern of irradiation.
Optimization of the Grashey View Radiograph for Critical Shoulder Angle Measurement: A Reliability Assessment With Zero Echo Time MRI
Authors: Yıldız Adalet Elçin , Yaraşır Yasin , Huri Gazi , Aydıngöz Üstün 
Published: August 2022

Background: Suboptimal positioning on Grashey view radiographs may limit the prognosticating potential of the critical shoulder angle (CSA) for shoulder disorders. Purpose: To investigate whether radiography optimized according to the latest research is reliable for measuring CSA in comparison with magnetic resonance imaging (MRI) featuring 3-dimensional (3D) zero echo time (ZTE) sequencing, which accentuates the contrast between cortical bone and surrounding soft tissue with high fidelity. Study Design: Cohort study (diagnosis); Level of evidence, 2. Methods: Patients with shoulder pain were prospectively and consecutively enrolled. All patients had Grashey view radiographs as well as 3.0-T MRI scans with isotropic 3D ZTE sequencing. Acceptable positioning on the radiographs was determined using the ratio of the transverse to longitudinal (RTL) diameter of the lateral glenoid outline; radiographs with an RTL ≥0.25 were repeated. Two observers independently measured the CSA on the radiographs and the coronal oblique reformatted ZTE images, the latter including verification of measurement points by cross-referencing against images from other planes. Reliability of measurements between observers and modalities was analyzed with the intraclass correlation coefficient (ICC). The paired-samples t test was used to compare the differences between imaging modalities. Results: Enrolled were 65 patients (35 female and 30 male; mean age, 40.2 years; range, 25-49 years). Radiographs with optimal positioning (RTL < 0.25) were attained after a mean of 1.6 exposures (range, 1-4); the mean RTL was 0.09 (range, 0-0.20). Interobserver agreement of CSA was excellent for radiographs (ICC = 0.91; 95% CI, 0.84-0.94) and good for ZTE MRI scans (ICC = 0.85; 95% CI, 0.71-0.92). Intermodality agreement of CSA between radiographs and ZTE MRI scans was moderate (ICC = 0.66; 95% CI, 0.48-0.73). The CSA was significantly different between an optimal radiograph (30.7° ± 4.3°) and ZTE MRI scan (31.8° ± 3.8) ( P = .005). Subgroup analysis revealed no significant differences in CSA measurement between ZTE MRI scans and Grashey view radiographs with an RTL of <0.1 ( P = .08). Conclusion: CSA measurement on ZTE MRI scans with anatomic point cross-referencing was significantly different from that on Grashey view radiographs, even with optimal positioning, and radiography may necessitate more than 1 exposure. An RTL of <0.1 ensured reliability of radiographs when other standards of sufficient x-ray exposure were met.
The effects of watering on cambial activity in the stems of evergreen hardwood (Samanea saman) during the pre-monsoon season in subtropical Bangladesh
Authors: Rahman Md Hasnat , Begum Shahanara , Nugroho Widyanto Dwi , Nakaba Satoshi , Funada Ryo 
Published: August 2022

AbstractWater stress has a significant impact on tree growth. However, the effects of watering on cambial activity and its influence on tree growth in subtropical climates is poorly understood. The present study analyzed the cambial activity on the stem of evergreen hardwood Samanea saman in response to either high frequency or low frequency watering during the pre-monsoon season in subtropical Bangladesh. We used two groups of seedlings: one group of seedlings was watered daily (high frequency watering), while the second group of seedlings was watered at 4–5-day intervals (low frequency watering). Samples for sequential observations of cambial activity by microscopy were collected from the main stems of seedlings of both groups. At the start of the experiment on March 25, 2015, during the pre-monsoon season, the cambium was inactive with no evidence of cell division. After 10 days of high frequency watering, cambial cell division and xylem differentiation were initiated. New cell plates were formed in the phloem side of the cambium. However, the cambium was inactive when low frequency watering was supplied. Supplying water in high frequency reactivated the cambium with forming small to large vessels. In contrast, the cambium remained inactive when low frequency watering was supplied throughout the experiment. These results suggest that continuous supply of water to the soil is one of the most important factors for cambial reactivation during pre-monsoon season in subtropical trees. Furthermore, our findings of artificial watering treatments might help to better understand the response of cambium to changes in precipitation patterns under natural conditions, allowing us to learn more about how cambium of subtropical trees responds to climate change.
Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus
Authors: Shabbir Ammarah , Batool Wajjiha , Yu Dan , Lin Lili , An Qiuli , Xiaomin Chen , Guo Hengyuan , Yuan Shuangshuang , Malota Sekete , Wang Zonghua , Norvienyeku Justice 
Published: August 2022

AbstractEndo-β-1,4-Xylanases are a group of extracellular enzymes that catalyze the hydrolysis of xylan, a principal constituent of the plant primary cell wall. The contribution of Endo-β-1,4-Xylanase I to both physiology and pathogenesis of the rice blast fungus M. oryzae is unknown. Here, we characterized the biological function of two endoxylanase I (MoXYL1A and MoXYL1B) genes in the development of M. oryzae using targeted gene deletion, biochemical analysis, and fluorescence microscopy. Phenotypic analysis of ∆Moxyl1A strains showed that MoXYL1A is required for the full virulence of M. oryzae but is dispensable for the vegetative growth of the rice blast fungus. MoXYL1B, in contrast, did not have a clear role in the infectious cycle but has a critical function in asexual reproduction of the fungus. The double deletion mutant was severely impaired in pathogenicity and virulence as well as asexual development. We found that MoXYL1A deletion compromised appressorium morphogenesis and function, leading to failure to penetrate host cells. Fluorescently tagged MoXYL1A and MoXYL1B displayed cytoplasmic localization in M. oryzae, while analysis of MoXYL1A-GFP and MoXYL1B-GFP in-planta revealed translocation and accumulation of these effector proteins into host cells. Meanwhile, sequence feature analysis showed that MoXYL1A possesses a transient chloroplast targeting signal peptide, and results from an Agrobacterium infiltration assay confirmed co-localization of MoXYL1A-GFP with ChCPN10C-RFP in the chloroplasts of host cells. MoXYL1B, accumulated to the cytoplasm of the host. Taken together, we conclude that MoXYL1A is a secreted effector protein that likely promotes the virulence of M. oryzae by interfering in the proper functioning of the host chloroplast, while the related xylanase MoXYL1B does not have a major role in virulence of M. oryzae.
Primitive neuroectodermal tumor of the kidney: an experience of three cases
Authors: Sharma Ankitkumar , Kuppurajan N. , Anandan M. , Madhavan Devdas , P. B Barani Kumar 
Published: August 2022

Abstract
Background
Primitive neuroectodermal tumors (PNET) are highly aggressive tumors belonging to the Ewing’s sarcoma family. Primitive neuroectodermal tumor is a group of bone and soft tissue tumor arising from neuroectodermal tissues. They are characterized by the expression of MIC2 and neural markers like neuron-specific enolase, synaptophysin, S-100, vimentin, Leu-7, and the presence of the EWS-FLI1 translocation. It is a rare malignancy with only about 150 cases being published worldwide. The tumor usually presents with non-specific symptoms such as abdomen pain and hematuria. Inferior vena cava (IVC) involvement by renal PNET is extremely rare with only a handful cases reported in the literature and is a clinically challenging scenario. There is a dearth of data on the management of this disease; further, the management of this disease in current scenarios is not well reported. We report our experience with three cases over 11 years which spans all three spectrums of the disease: localized, locally advance and metastasis.

Case presentation
Case 1: 37-year-old gentleman presenting with hematuria found to have right renal tumor with IVC invasion which was found to be unresectable intraoperatively. Patient was started on chemotherapy but deteriorated and died 3 months later. Case 2: 38-year-old gentleman presenting with abdominal pain found to have locally advanced left renal tumor. Patient developed local recurrence and metastasis 15 months after resection but had stable disease for nearly 4 years before developing acute myeloid leukemia and withdrawing consent for treatment. Case 3: 13-year-old girl who presented with right abdominal mass and subsequently found to have localized disease. After aggressive chemotherapy, patient is now recurrence-free after nearly 11 years.

Conclusions
Our study aims to show the spectrum of renal primitive neuroectodermal tumors with outcomes in all three clinical scenarios, with the aim of reinforcing that high index of suspicion for this disease is required in young patients presenting with aggressive disease, and immunohistochemistry should be used when in doubt, as outcomes are greatly improved with adjuvant chemotherapy.
Prognostic value of ambulation ability with albumin and C-reactive protein to predict 28-day mortality in elderly sepsis patients: a retrospective multicentre registry-based study
Authors: Cha Kyungman , Choi Seung Pill , Kim Soo Hyun , Oh Sang Hoon 
Published: August 2022

Abstract
Background
Sepsis is a series of organ failures caused by dysregulated responses to infection. Risk factors for sepsis are multiple comorbidities, a poor nutrition status, and limited mobility. The primary purpose of the study was to determine whether ambulation ability with albumin and C-reactive protein are predictive of 28-day mortality of elderly patients with sepsis.

Methods
This was a retrospective observational study using a multicentre-based registry of elderly patients between November 2016 and February 2017. The inclusion criteria were a patient ≥65 years and a diagnosis of sepsis and exclusion criteria were a patient with covariates of ambulation ability such as central nervous system diseases, or malignancy. The area under the receiver operating characteristic curve of prediction models were calculated and compared. The survival rates according to the ambulation ability were estimated and compared by the log-rank test.

Results
2291 patients ≥65 years visited with infectious diseases. 496 subjects with central nervous system diseases, 710 subjects with malignancy and 817 subjects with a Sequential Organ Failure Assessment score ≤ 1 were excluded. Ultimately, 278 subjects were included in the primary analysis. 133 (47.8%) subjects were male and the median age was 78 years. 228 (82%) subjects could ambulate independently before morbidity and 28 (10.1%) subjects expired in 28 days. In the inability to ambulate and C-reactive protein to albumin ratio model, the area under the curve predicting 28-day mortality was 0.761 with no significant difference from the Sequential Organ Failure Assessment score (0.859, p = 0.097) and the estimated survival rate on 28th day according to the ability to ambulate showed a significant difference (hazard ratio = 1.212, p < 0.001).

Conclusion
The premorbid ambulation ability with albumin and C-reactive protein can be combined to predict 28-day mortality in elderly patients with sepsis.