bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implantable collamer lens implantation (ICL) versus small incision lenticule extraction (SMILE) in low to moderate myopia: study protocol for a randomized, non-inferiority trial
Authors: Li Kangjun , Wang Zheng , Wang Ming X. 
Published: October 2022

Abstract

Background
Implantable collamer lens implantation (ICL) is a form of ‘foldable’ posterior chamber phakic intraocular lens refractive surgery that generally does not impair cornea and natural accommodation. The potential advantages of the ICL over keratorefractive laser procedures include less induction of higher-order aberrations (HOAs) and enhanced retinal image magnification. On the other hand, small incision lenticule extraction (SMILE), currently, one of the most popular refractive surgery procedures, also offers excellent visual outcomes, particularly for eyes with low to moderate amounts of myopia. The aim of this study is to evaluate whether ICL/TICL (toric ICL) is comparable to SMILE for low to moderate myopia in terms of refractive outcomes at 3 and 18 months post-operatively.

Methods/design
This is a prospective randomized study. A total of 300 participants will be randomized into two groups, the ICL/TICL group and SMILE group. Eligible participants with spherical equivalent (SE) less than − 6.0 diopter (D) will be recruited. Following randomization, participants will be followed at 1, 3, 6, 12, and 18 months. The primary outcome is the refractive predictability at every postoperative point after surgery, which is the proportion of the number of eyes achieving a postoperative SE within ± 0.5 D and ± 1.0 D of the intended target. Secondary outcome parameters include visual acuity, refraction, adverse events, and quality of vision measurements.

Discussion
This trial will provide information on whether ICL has comparable, if not superior, refractive outcomes compared to the established SMILE for low to moderate myopia, thus providing evidence for translation into clinical practice.

Trial registration
Chinese clinical trial registry (ChiCTR) 2200055372. Registered on 08 January 2022.
Seasonal fluctuations of Babesia bigemina and Rhipicephalus microplus in Brangus and Nellore cattle reared in the Cerrado biome, Brazil
Authors: Martins Kauê Rodriguez , Garcia Marcos Valério , Bonatte-Junior Paulino , Duarte Pâmella Oliveira , Csordas Barbara Guimarães , Higa Leandro de Oliveira Souza , Zimmermann Namor Pinheiro , Barros Jacqueline Cavalcante , Andreotti Renato 
Published: October 2022

Abstract
Background
The tick Rhipicephalus microplus (Ixodida: Ixodidae, Canestrini, 1888) causes substantial economic and health losses in cattle production and is the main vector of Babesia bigemina (Piroplasmidae: Babesidae, Smith & Kilborne, 1893). Babesia bigemina is responsible for a tick-borne disease known as babesiosis that can cause hemolytic anemia, fever and death. In the study reported here, we investigated the relationship between the number of ticks per animal and the number of B. bigemina cytochrome b gene (cbisg) copies in the blood of Brangus and Nellore cattle reared without acaricidal treatment in the Brazilian Cerrado biome over a 1-year period.

Methods
Ticks on 19 animals (9 Brangus and 10 Nellore cattle) were counted every 18 days, and blood was collected every 36 days for 12 months. Serological samples were analyzed with an indirect enzyme-linked immunosorbent assay, and genomic DNA was analyzed by conventional PCR and quantitative PCR. The PCR products were sequenced by the Sanger method.

Results
The Brangus and Nellore breeds showed similar weight development and no clinical signs of babesiosis. Statistically significant differences (P < 0.05) between the breeds were observed for the number of ticks and the number of B. bigemina cbisg gene copies.

Conclusions
No correlation between the number of ticks and the number of circulating copies of cbisg was observed, although Nellore cattle presented with fewer ticks than Brangus cattle and the number of cbisg copies was higher for Nellore cattle than for Brangus cattle.
Cystic echinococcosis in northern Tanzania: a pilot study in Maasai livestock-keeping communities
Authors: Tamarozzi Francesca , Kibona Tito , de Glanville William A. , Mappi Tauta , Adonikamu Elly , Salewi Anande , Misso Kennedy , Maro Venance , Casulli Adriano , Santoro Azzurra , Santolamazza Federica , Mmbaga Blandina T. , Cleaveland Sarah 
Published: October 2022

Abstract
Background
There are close similarities between the life-cycles of Echinococcus granulosus sensu lato (E. granulosus s.l.) that causes cystic echinococcosis (CE) in humans and Taenia multiceps/Coenurus cerebralis that causes cerebral coenurosis in small ruminants. Recent evidence highlights that livestock in Maasai communities of northern Tanzania are suffering from increases in the prevalence of cerebral coenurosis, leading to concerns about a possible concurrent increased risk of human CE. The aim of this study was to estimate the prevalence of human abdominal CE and the prevalence and species/genotypes of E. granulosus s.l. in livestock in Maasai communities.

Methods
Human CE was diagnosed by abdominal ultrasound on volunteers aged ≥ 7 years in five villages in the Longido and Ngorongoro Districts in northern Tanzania. Infection in ruminants was evaluated through inspection in local abattoirs, followed by molecular identification of one cyst per animal, with a priority for hepatic cysts, using PCR targeting of the cytochrome c oxidase I gene (COX1), followed by restriction fragment length polymorphism and multiplex PCR, and sequencing of non-E. granulosus s.l. samples.

Results
Ultrasound was performed on 823 volunteers (n = 352 in two villages in Longido District, and n = 471 in three villages of Ngorongoro). Hepatic CE cases were diagnosed only in Ngorongoro (n = 6; 1.3%), of which three had active cysts. Village-level prevalence of CE ranged between 0 and 2.4%. Of the 697 ruminants inspected, 34.4% had parasitic cysts. Molecular identification was achieved for 140 of the 219 (63.9%) cysts sampled. E. granulosus s.l. and T. hydatigena/Cysticercus tenuicollis were identified in 51.4% and 48.6%, respectively, of livestock cysts. E. granulosus s.l. was identified in livestock from both Longido (35.3% of 116 genotyped cysts) and Ngorongoro (91.2% of 34 genotyped cysts). Of the total of 72 E. granuslosus s.l. cysts identified in livestock, 87.5% were E. granulosus sensu stricto (G1–G3 genotypes), 9.7% were E. ortleppi (G5) and one cyst was E. canadensis (G6–10). The three active human cysts, which were removed surgically, were G1–G3 genotypes.

Conclusions
Multiple species/genotypes of E. granulosus s.l. are circulating in Maasai communities of northern Tanzania. Human CE was detected in villages of Ngorongoro District and a high prevalence of echinococcal cysts was observed in livestock in both districts. More precise estimation of the prevalence in this area and a better understanding of the specific risk factors for CE among Maasai communities in northern Tanzania is needed. Interventions targeting transmission routes common to both E. granulosus s.l. and T. multiceps would have dual benefits for preventing both human and livestock disease.

Graphical Abstract
Differentiating tracheobronchial involvement in granulomatosis with polyangiitis and relapsing polychondritis on chest CT: a cohort study
Authors: Jalaber Carole , Puéchal Xavier , Saab Ines , Canniff Emma , Terrier Benjamin , Mouthon Luc , Cabanne Eglantine , Mghaieth Sandra , Revel Marie-Pierre , Chassagnon Guillaume 
Published: October 2022

Abstract
Background
In patients with tracheobronchial involvement, the differential diagnosis between granulomatosis with polyangiitis (GPA) and relapsing polychondritis (RP) can be challenging. The aim of this study was to describe the characteristics of airway abnormalities on chest computed tomography (CT) in patients with GPA or RP and to determine whether specific imaging criteria could be used to differentiate them.

Methods
GPA and RP patients with tracheobronchial involvement referred to a national referral center from 2008 to 2020 were evaluated. Their chest CT images were reviewed by two radiologists who were blinded to the final diagnosis in order to analyze the characteristics of airway involvement. The association between imaging features and a diagnosis of GPA rather than RP was analyzed using a generalized linear regression model.

Results
Chest CTs from 26 GPA and 19 RP patients were analyzed. Involvement of the subglottic trachea (odds ratio for GPA=28.56 [95% CI: 3.17; 847.63]; P=0.001) and extensive airway involvement (odds ratio for GPA=0.02 [95% CI: 0.00; 0.43]; P=0.008) were the two independent CT features that differentiated GPA from RP in multivariate analysis. Tracheal thickening sparing the posterior membrane was significantly associated to RP (odds ratio for GPA=0.09 [95% CI: 0.02; 0.39]; P=0.003) but only in the univariate analysis and suffered from only moderate interobserver agreement (kappa=0.55). Tracheal calcifications were also associated with RP only in the univariate analysis (odds ratio for GPA=0.21 [95% CI: 0.05; 0.78]; P=0.045).

Conclusion
The presence of subglottic involvement and diffuse airway involvement are the two most relevant criteria in differentiating between GPA and RP on chest CT. Although generally considered to be a highly suggestive sign of RP, posterior tracheal membrane sparing is a nonspecific and an overly subjective sign.
Evaluation of cross-platform compatibility of a DNA methylation-based glucocorticoid response biomarker
Authors: Tang Emily , Wiencke John K. , Warrier Gayathri , Hansen Helen , McCoy Lucie , Rice Terri , Bracci Paige M. , Wrensch Margaret , Taylor Jennie W. , Clarke Jennifer L. , Koestler Devin C. , Salas Lucas A. , Christensen Brock C. , Kelsey Karl T. , Molinaro Annette M. 
Published: October 2022

Abstract
Background
Identifying blood-based DNA methylation patterns is a minimally invasive way to detect biomarkers in predicting age, characteristics of certain diseases and conditions, as well as responses to immunotherapies. As microarray platforms continue to evolve and increase the scope of CpGs measured, new discoveries based on the most recent platform version and how they compare to available data from the previous versions of the platform are unknown. The neutrophil dexamethasone methylation index (NDMI 850) is a blood-based DNA methylation biomarker built on the Illumina MethylationEPIC (850K) array that measures epigenetic responses to dexamethasone (DEX), a synthetic glucocorticoid often administered for inflammation. Here, we compare the NDMI 850 to one we built using data from the Illumina Methylation 450K (NDMI 450).

Results
The NDMI 450 consisted of 22 loci, 15 of which were present on the NDMI 850. In adult whole blood samples, the linear composite scores from NDMI 450 and NDMI 850 were highly correlated and had equivalent predictive accuracy for detecting DEX exposure among adult glioma patients and non-glioma adult controls. However, the NDMI 450 scores of newborn cord blood were significantly lower than NDMI 850 in samples measured with both assays.

Conclusions
We developed an algorithm that reproduces the DNA methylation glucocorticoid response score using 450K data, increasing the accessibility for researchers to assess this biomarker in archived or publicly available datasets that use the 450K version of the Illumina BeadChip array. However, the NDMI850 and NDMI450 do not give similar results in cord blood, and due to data availability limitations, results from sample types of newborn cord blood should be interpreted with care.
Bobble-head doll syndrome in an infant with an arachnoid cyst: a case report
Authors: Doya Leen Jamel , Kadri Hassan , Jouni Oday 
Published: October 2022

Abstract
Background
Bobble-head doll syndrome is a rare and unique movement disorder most commonly affecting children younger than 5 years of age. It is characterized by continuous or episodic movement at the frequency of 2–3 Hz. The exact mechanism of bobble-head doll syndrome has not been elucidated. Endoscopic ventriculocisternostomy is the optimal treatment option. In a literature review, there were less than 75 cases of bobble-head doll syndrome with suprasellar arachnoid cyst.

Case presentation
We report a case of a 1.5-year-old Asian-Syrian girl who presented with a history of excessive head nodding for 3 months that increased with walking, emotions, and stress; decreased during periods of concentration; and was absent during sleep. On physical examination, she was alert and normal, with no medical history. Laboratory assessment and ophthalmological examination were normal. Cranial magnetic resonance imaging demonstrated a well-defined thin-walled suprasellar arachnoid cyst measuring 3 × 5 × 7 cm that obstructed the foramina of Monro, with resulting hydrocephalus ventriculomegaly. The patient underwent endoscopic cystoventriculostomy and cystocisternostomy for the suprasellar arachnoid cyst. During the 6 months of follow-up, the head bobbing disappeared completely, and her growth was normal.

Conclusion
Despite the rareness of bobble-head doll syndrome, it is considered an important condition that must be investigated early to detect the cause and treated promptly to avoid potential complications.
Influencing factors of biliary fistula after radical resection of hilar cholangiocarcinoma: a prospect cohort
Authors: Zhang Dengyong , Qi Feiyu , Sun Wanliang , Zhao Guanru , Wang Dongdong , Zhou Shuo , Liu Zhong , Lu Zheng 
Published: October 2022

Abstract
Background
Biliary fistula is a common but serious complication after radical resection of hilar cholangiocarcinoma. We aimed to evaluate the influencing factors of biliary fistula after radical resection, to provide insights to the clinical treatment of hilar cholangiocarcinoma.

Methods
Patients undergoing radical resection of hilar cholangiocarcinoma from January 1, 2015 to March 31, 2022 were selected. Patients’ personnel characteristics and laboratory test results of patients with and without biliary fistula were collected and compared. Logistic regression analyses were conducted to evaluate the associated risk factors of biliary fistula.

Results
160 patients undergoing radical resection of hilar cholangiocarcinoma were included, the incidence of postoperative biliary fistulas was 20.63%. There were significant differences in the age, preoperative cholangitis and number of biliary anastomosis between biliary fistula and no biliary fistula patients (all p < 0.05). There were significant differences in the gamma glutamyl transpeptidase (GGT) on the first day after surgery, Klebsiella pneumoniae between biliary fistula and no biliary fistula patients (all p < 0.05). Logistic regression analysis indicated that age ≥ 65 years (OR  2.035, 95%CI 1.131–3.007), preoperative cholangitis (OR 1.584, 95% CI 1.081–2.361), number of biliary anastomosis ≥ 2(OR 2.866, 95%CI 1.942–3.624), GGT on the first day after surgery ≥ 120 U/L (OR 1.823, 95%CI: 1.274–2.906), preoperative bile culture for Klebsiella pneumoniae (OR 3.181, 95%CI: 2.426–3.992) were the risk factors of postoperative biliary fistulas (all p < 0.05).

Conclusions
There are many independent risk factors for postoperative biliary fistula in patients undergoing radical resection of hilar cholangiocarcinoma. Clinical medical workers should take early interventions and treatment measures for these high-risk patients to reduce the occurrence of postoperative biliary fistula.
An archaeometric assessment study of Seljuk period glazed tiles from Kılıçarslan Square (Konya, Turkey)
Authors: Öztürk Çetin , Şimşek Franci Gülsu , Kuşoğlu İhsan Murat 
Published: October 2022

Abstract
This study presents archaeometric analyses of glazed tiles produced with the cut-mosaic technique to reveal information about the Anatolian Seljuk period’s architecture and ceramic technology. The Persian Seljuk artists also used the same technique. For this purpose, physical, chemical, thermal, mineralogical, microstructural, and molecular analyses were carried out on the tiles from the Seljuk period unearthed in the Kılıçarslan Square excavation in Konya, the capital of the Anatolian Seljuk State from the twelfth-century to the beginning of the fourteenth-century. SEM, XRD, and Heat Microscopy analyses showed that the Seljuk period tiles were not fired at high temperatures like today’s tiles and were probably fired at temperatures below 1100 °C. WD-XRF analyses revealed that the glazes are classified into two categories, one being alkali varying the content of Na2O + K2O between 13 and 16 wt% (PbO between 0.5 and 2 wt%) and the other being lead-alkali type in which PbO content varies between 13 and 15 wt% and alkali content 10–14 wt%. The cobalt content in the blue color is around 0.2 wt%, and copper content in the turquoise-colored samples varies between 2.9 and 4.4 wt% depending on the lightness and darkness of the color (higher amount for tile 1 and tile 3, which are darker than the others). The brown color is obtained by the presence of MnO (3.3 wt%). Raman analysis showed that the difference in sintering temperature caused the color tone difference in Seljuk period tiles, and glazes were formed in the range of 800–1000 °C depending on the Ip values.
Performance of a hybrid heating system based on enhanced deep borehole heat exchanger and solar energy
Authors: He Yujiang , Bu Xianbiao 
Published: October 2022

AbstractDeep borehole heat exchanger (DBHE) is a closed loop system without the problem of fluid losses, scale formation and corrosion; however, low rock thermal conductivity limits its performance. Enlightened by drilling mud loss in oil and gas industry, here an enhanced DBHE (EDBHE) is proposed by filling materials with much higher thermal conductivity into leakage formation or depleted gas and oil reservoir to enhance the thermal conductivity performance of rock. Solar thermal energy is stored into EDBHE during the non-heating season to replenish the loss of heat energy extracted during the heating season. The results show that average heat mining rate for 20 years operations is, respectively, 3686.5 and 26,384.4 kW for EDBHE filled by ordinary drilling mud and by composite materials with high thermal conductivity. The percentage reduction of heat mining rate for 20 years operations for EDBHE and the hybrid system of geothermal and solar energy are, respectively, 16.1 and 5.8%, indicating that the hybrid system can make the heat mining rate more stable.
Variability in metabolites produced by Talaromyces pinophilus SPJ22 cultured on different substrates
Authors: Adelusi Oluwasola Abayomi , Gbashi Sefater , Adebiyi Janet Adeyinka , Makhuvele Rhulani , Adebo Oluwafemi Ayodeji , Aasa Adeola Oluwakemi , Targuma Sarem , Kah Glory , Njobeh Patrick Berka 
Published: October 2022

Abstract
Background
Several metabolites released by fungal species are an essential source of biologically active natural substances. Gas chromatography high resolution time-of-flight mass spectrometry (GC-HRTOF-MS) is one of the techniques used in profiling the metabolites produced by microorganisms, including Talaromyces pinophilus. However, there is limited information regarding differential substrates’ impacts on this fungal strain’s metabolite profiling. This study examined the metabolite profile of T. pinophilus strain SPJ22 cultured on three different media, including solid czapek yeast extract agar (CYA), malt extract agar (MEA) and potato dextrose agar (PDA) using GC-HRTOF-MS. The mycelia including the media were plugged and dissolved in 5 different organic solvents with varying polarities viz.: acetonitrile, dichloromethane, hexane, 80% methanol and water, and extracts analysed on GC-HRTOF-MS.

Results
The study revealed the presence of different classes of metabolites, such as fatty acids (2.13%), amides (4.26%), alkanes (34.04%), furan (2.13%), ketones (4.26%), alcohols (14.89%), aromatic compounds (6.38%), and other miscellaneous compounds (17.02%). Significant metabolites such as acetic acid, 9-octadecenamide, undecanoic acid methyl ester, hydrazine, hexadecane, nonadecane, eicosane, and other compounds reported in this study have been widely documented to have plant growth promoting, antimicrobial, anti-inflammatory, antioxidant, and biofuel properties. Furthermore, T. pinophilus grown on PDA and MEA produced more than twice as many compounds as that grown on CYA.

Conclusion
Thus, our result showed that the production of essential metabolites from T. pinophilus is substrate dependent, with many of these metabolites known to have beneficial characteristics, and as such, this organism can be utilised as a sustainable and natural source for these useful organic molecules.
Strengthening surveillance in Ghana against public health emergencies of international concern
Authors: Adachi Motoi , Taniguchi Kiyosu , Hori Hiroki , Mizutani Taketoshi , Ishizaka Aya , Ishikawa Koichi , Matano Tetsuro , Opare David , Arhin Doris , Asiedu Franklin Bekoe , Ampofo William Kwabena , Yeboah Dorothy Manu , Koram Kwadwo Ansah , Anang Abraham Kwabena , Kiyono Hiroshi 
Published: October 2022

AbstractAmong western African countries, the Republic of Ghana has maintained an economic growth rate of 5% since the 1980s and is now categorized as a middle-income country. However, as with other developing countries, Ghana still has challenges in the effective implementation of surveillance for infectious diseases. Facing public health emergencies of international concern (PHEIC), it is crucial to establish a reliable sample transportation system to the referral laboratory. Previously, surveillance capacity in Ghana was limited based on Integrated Disease Surveillance and Response, and therefore the “Surveillance and Laboratory Support for Emerging Pathogens of Public Health Importance in Ghana (SLEP)” was introduced to strengthen diarrhea surveillance. The SLEP project started with a sentinel diarrhea survey supported by SATREPS/JICA in collaboration with National Public Health Reference Laboratory (NHPRL) and Noguchi Memorial Institute of Medicine (NMIMR). The base-line survey revealed the limited capacity to detect diarrhea pathogens and to transfer samples from health centers to NHPRL. The involvement of private clinic/hospital facilities into the surveillance network is also crucial to strengthen surveillance in Ghana. The strong and interactive relationship between the two top referral laboratories, NHPRL under the Ministry of Health NMIMR and under the Ministry of Education, enables Ghana Health Services and is critical for the rapid response against PHEIC. In future, we hope that the outcome of the SLEP surveillance project could contribute to building a surveillance network with more timely investigation and transfer of samples to referral labs.