bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of “PCMC+Scenario simulation” PAD class mode on midwifery students
Authors: Wang Yuji , Yang Jianying , Huang Yan 
Published: November 2022

Abstract
Objective: To investigate the effect of “PCMC (Problem-originated Clinical Medical Curriculum) + Scenario simulation” PAD (presentation–assimilation–discussion) class mode in the teaching of midwifery students.
Backgroud: Assessment ability is the core of midwives’ effective judgment and treatment of clinical problems. The clinical assessment ability of midwifery students in China is mostly at a medium or low level. Improving clinical assessment ability, especially critical thinking of midwifery students, is of great significance to midwifery practice education.
Method: A total of 104 midwifery students in four grades of West China School of Nursing, Sichuan University were selected as the research objects. Grades 16 and 17 were the control group for routine teaching in Midwifery Health Assessment, and Grades 18 and 19 were for the experimental group, using the "PCMC + Scenario simulation" PAD class mode, and a questionnaire survey was conducted after the intervention.
Results: After intervention, the critical thinking ability of the experimental group was higher than the control group (284.81±27.98 and 300.94±31.67, p=0.008). And the four dimensions of Open-mindedness (40.56± 5.60and 43.59± 4.90, p=0.005), Analyticity (42.83± 5.17and 45.42± 5.72, p=0.020), Systematicity (38.79±4.70 and 41.88±6.11, p=0.006), and Critical thinking self-confidence (41.35± 5.92 and43.83±5.89, p=0.039) were higher than the control group. And the course satisfaction of the experimental group was higher than the control group (84.81± 8.49 and 90.19± 8.41, p = 0.002).
Conclusion: The “PCMC + Scenario simulation” PAD class mode can improve the critical thinking ability of midwifery students. And enhance the curriculum satisfaction.
The Effect of Guided group Reflection on the Ability and Convenience of Breaking Bad News in Pre-hospital Emergency Staff
Authors: Zarezadeh Mohammad Reza , Mirzaei Samaneh , Mirjalili Seyed Mohammad Javad , Nasiriani Khadijeh 
Published: November 2022

Abstract
Background:Bad news refers to the category of news that create negative changes in a person's understanding or expectations of his present and future. Breaking bad news (BBN) is a stressful task that may have disturbing effects on the professional performance and general health of the medical staff. Pre-hospital emergency staff often needs to break bad news to the patient or his family. This study was conducted to determine the effect of Guided group Reflection training on the ability and comfort of BBN in pre-hospital emergency staff.
Methods:This quasi-experimental study was conducted on a total of 95 operational staff of the pre-hospital emergency ward in Yazd, Iran, in two groups: the case and the control groups. For the case group, a 4-hour training workshop on BBN was held, and then a group was formed in virtual space to discuss and exchange opinions about the scenarios of encountering and reflecting on it. Data collection tools were SPIKES Questionnaire and the Convenience of Breaking Bad News Questionnaire. The data were analyzed with SPSS.
Results: The mean score of the ability to break bad news after the intervention was 44.01±6.21 in the case group and 31.40±4.51 in the control group, and a significant difference was found using the independent t-test (P=0.0001). Besides, the mean scores of the convenience of BBN in post-test was 5.52±1.64 in the case group and 3.50±1.28 in the control group using the independent t-test with a significant difference (P=0.0001).
Conclusion:According to the findings, training in Guided group Reflection improved the ability to report bad news and its convenience in pre-hospital emergency staff; so, the use of this method in training and retraining for this group is suggested.
Breakthrough: A First-In-Class Virtual Simulator for Dose Optimization of ACE Inhibitors in Translational Cardiovascular Medicine
Authors: Schneider Benjamin K. , Ward Jessica , Sotillo Samantha , Garelli-Paar Catherine , Guillot Emilie , Prikazsky Marc , Mochel Jonathan P. 
Published: November 2022

Abstract

The renin-angiotensin-aldosterone-systems (RAAS) play a central role in the pathophysiology of congestive heart failure (CHF), justifying the use of angiotensin converting enzyme inhibitors (ACEi) in dogs and humans with cardiac diseases. Seminal studies in canine CHF had suggested that the pharmacological action of benazepril was relatively independent of doses greater than 0.25 mg/kg P.O, thereby providing a rationale for the European labeled dose of benazepril in dogs with CHF. However, most of these earlier studies relied on measures of ACE activity, a sub-optimal endpoint to characterize the effect of ACEi on the RAAS. The objectives of this study were (i) to expand on previous mathematical modeling efforts of the dose-exposure-response relationship of benazepril on biomarkers of the RAAS which are relevant to CHF pathophysiology and disease prognosis; and (ii) to develop a software implementation capable of simulating clinical trials in benazepril in dogs bedside dose optimization. Our results suggest that 0.5 mg/kg PO q12h of benazepril produces the most robust reduction in angiotensin II and upregulation of RAASalternative pathwaybiomarkers. This model will eventually be expanded to include relevant clinical endpoints, which will be evaluated in an upcoming prospective trial in canine patients with CHF.
Comparison of hook wire combined with indocyanine green and hook wire alone for localization of pulmonary nodules in TV-assisted thoracoscopic surgery
Authors: Qian Jianhua , Huang Bo 
Published: November 2022

Abstract
Objectives：Comparison of hook wire combined with indocyanine green（ICG） and hook wire alone for localizing pulmonary nodules in TV-assisted thoracoscopic surgery（VATS).
Methods：From September 2020 to October 2022, a total of 117 patients underwent computed tomography-guided localization of pulmonary nodules prior to VATS at the same medical institution. Among them, 42 patients received dual localization with hook wire combined with ICG, constituting a dual localization group, and 75 patients received only hook wire localization, constituting a single localization group. We performed a retrospective comparative analysis of the preoperative localization and major intraoperative problems in these 117 patients.
Results：No serious complications occurred in either positioning group. The cough complication rate was higher in the dual localization group (9.5% versus 0.0%, P=0.015). The difference in positioning time between the two groups was not statistically significant (P = 0.452). Seven patients in the single localization group required extended pneumonectomy compared with none in the dual localization group (9.3% versus 0.0%, P = 0.048).
Conclusions：Hook wire combined with ICG increases cough complications compared to hook wire alone for localizing pulmonary nodules but is effective in reducing the enlargement of the pneumonectomy.
Validated negative regions (VNRs) in the VISTA database might be truncated forms of bona fide enhancers
Authors: Ni Pengyu , Su Zhengchang 
Published: November 2022

Abstract

The VISTA enhancer database is a valuable resource for evaluating predicted regulatory sequences and studying various aspects of enhancers in mammalian genomes. In addition to thousands of validated positive regions (VPRs) in the human and mouse genomes, the database also contains similar numbers of validated negative regions (VNRs). We find that like the VPRs, the VNRs also are under strongly evolutionary constraints and are almost completely recalled by highly accurately predicted cis-regulatory modules (CRMs) in the genomes. However, both VNRs and VPRs are on average half as long as the recalling CRMs, while containing a small portion of non-enhancer sequences. These results suggest that both VNRs and VPRs might be truncated forms of long enhancers. It appears that VPRs might be at least partially functional in their truncated form under the assessed conditions, while VNRs might not be functional in their truncated forms and/or under the assessed conditions.
Pump it up: bioelectric stimulation controls tissue hydrostatic
pressure and water transport via electro-inflation
Authors: Shim Gawoon , Breinyn Isaac , Martinez-Calvo Alejandro , Cohen Daniel 
Published: November 2022

Abstract
Epithelial tissues sheath many organs, separating ‘outside’ from ‘inside’ and exquisitely regulating ion and water transport via electromechanical pumping to maintain homeostatic balance and tissue hydrostatic pressure. While it is becoming increasingly clear that the ionic microenvironment and external electric stimuli can affect epithelial function and behavior, the coupling between electrical perturbation and tissue form remain unclear. We investigated this by combining electrical stimulation with three-dimensional epithelial tissues with hollow ‘lumens’—both kidney cysts and complex intestinal stem cell organoids. Our core finding is that physiological strength electrical stimulation of order 1-3 V/cm (with both direct and alternating currents) can drive powerful and rapid inflation of hollow tissues through a process we call ‘electro-inflation’, inducing a nearly threefold increase in tissue volume and striking asymmetries in tissue form. Electro-inflation is primarily driven by activation of the CFTR ion channel pumping chloride into the tissue, causing water to osmotically flow. This influx generates hydrostatic pressure, and inflation results from a competition between this pressure and cell cytoskeletal tension. We validated these interpretations with computational models connecting ion dynamics in the Diffuse Double Layer around tissues to tissue mechanics. Electro-inflation is a unique biophysical process to study and control complex tissue function.
Attitude towards Safe Abortion and Associated Factors among Health Care Professionals at the Public Health Facilities of Bahir Dar city, Northwest, Ethiopia, 2021
Authors: Tadie Abebay , Mekonen Aemtsehay , Belachew Melash , Feyisa Wondu , Alemnew Fentahun , Tebabal Amanuel , Abie Alemwork 
Published: November 2022

Abstract
Background
Negative attitude towards abortion among health care professionals providing abortion services could be an obstacle even under a law, which permits abortion on request. Health care providers are expected to perform and be change agents of abortion services. However, little information is known about the attitude towards safe abortion among health care providers in Ethiopia.
Objective
This study aimed to assess attitude towards safe abortion and associated factors among health care providers in the public health facilities of Bahir Dar city, Northwest Ethiopia.
Methods
A health facility-based cross-sectional study was employed from March 1–30/2021 among 416 health care providers. The data were collected by computer-based generated simple random sampling technique, entered, coded, and cleaned using Epi data version 4.2 and analyzed using Statistical Package of Social Sciences version 25.0. Bivariate and multivariable logistic regression analyses were employed to estimate the crude and adjusted odds ratio with a confidence interval of 95% and a P-value of less than 0.05 considered statistically significant. Frequency tables, figures, and descriptive summaries were used to describe the study variables.
Results
In this study, 70.2% [95% CI: 65.6–74.6] of health care professionals had favorable attitude towards safe abortion. The age group of 25–29, 30–34, and ≥ 35 years [AOR = 3.34, 95% CI = 1.03–10.85], [AOR = 4.58, 95% CI = 1.33–15.83] and [AOR = 5.30, 95% CI = 1.43–19.66] respectively, male health care providers [AOR = 3.20, 95% CI = 1.55–6.60], midwives [AOR = 6.50, 95% CI = 2.40-17.44], working at hospital [AOR = 4.77, 95% CI = 1.53–14.91], trained on abortion [AOR = 5.09, 95% CI = 2.29–11.32], practicing of an abortion procedure [AOR = 2.52, 95%, CI = 1.13–5.60], knowledge on safe abortion [AOR = 7.35, 95% CI = 3.23–16.71], awareness on revised abortion law [AOR = 6.44, 95% CI = 3.15–13.17] and need further legalization of abortion law [AOR = 11.78, 95% CI = 5.52–24.26] were associated with favorable attitude towards safe abortion.
Conclusion
In the present study, health care providers who had a favorable attitude towards safe abortion were relatively high compared to the previous studies. Age, sex, profession, working place, training, knowledge, and practice-related factors were associated with a favorable attitude towards safe abortion. Thus, health institutions considered a need for sensitization for health professionals about safe abortion to improve and sustain a positive attitude.
The significance of lysosome in the diagnosis and subclassification of Alzheimer's disease
Authors: Li Chenghao , Zheng Zhuo , Jin Meishan 
Published: November 2022

Abstract
Background: Alzheimer's disease (AD) is a heterogeneous disease with complex pathophysiological characteristics. Lysosomes are the main organelles of degradation in eukaryotic cells, and their dysfunction is closely related to AD. Therefore, our goal is to identify the lysosomal induced molecular subtype of AD and further explore the possible mechanisms.
Methods: The dataset was downloaded from the GEO database. By differential expression analysis, 50 differentially expressed lysosomal genes in AD were identified. R-package "ROCR" was used to plot and calculate ROC curves and AUC values for differential lysosomal genes. The expression data of the above genes with AUC greater than 0.7 in the AD group were extracted and the R package "ConsensusClusterPlus" was used for consistent clustering of the AD data set. The contents of 28 kinds of immune cells in all samples (cluster1:28, cluster2:28) of the AD dataset were calculated using the R-package "GSVA". The R package "limma" was used to analyze the differences of autophagy genes in 56 AD data sets based on consistent clustering. R package "WGCNA" carried out weighted co-expression network analysis of the differential genes between cluster1 and cluster2, and extracted the related genes of the two modules with the highest positive and negative correlation. GO and KEGG functional enrichment of the above module genes was performed.
Results: Two lysosomes subtypes (Cluster1:28, Cluster2: 28) with different outcomes were identified in AD cohort by unsupervised clustering of lysosome diagnostics molecular, known as cluster 1/2. The results showed that 13 immune cells were significantly different between cluster1 and cluster2. A total of 76 differentially expressed autopaghy genes were identified. It indicated autopaghy heterogeneity between lysosome subtypes. The enrichment analysis of the key module genes of the lysosome subtype showed that the key module genes were mainly concentrated in the gene set associated with the synapses.
Conclusions: According to the heterogeneity of lysosomes, we identified two different AD subtypes based on different lysosome gene expressions, preliminarily revealing that the heterogeneity of AD may be mainly caused by lysosomes. The role of lysosomes may be related to autophagy and synapses.
A novel sensor for antiviral drug Favipiravir in the treatment of COVID-19 designed by G/In 2 O 3 nanomaterial
Authors: Sanjeevagol Shaila , Manjanna Jayappa , Chaithra Rudrappa 
Published: November 2022

Abstract

The world health organization (WHO) declared that the outbreak of SARS-CoV-2 constituted a public health emergency of international concern. Thus, accredited fevipiravir (FVP) was one of the potential drugs for the treatment of Covid-19 infected patients. Hence it is of interest to study the electrochemical behavior of FVP. We report the performance of a low-cost composite of graphene and indium oxide (G/In2O3) nanomaterial drop casted on pencil graphite electrode employed to detect FVP. The G/In2O3 was prepared by a simple precipitation method. The electrochemical study of FVP was carried out by using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry. The G/In2O3 modified electrode detects FVP well in Britton Robinson (BR) buffer pH of 7.2. The oxidation peak was observed at the potential range of 1.0 to 1.23 V (vs. Ag/AgCl). The linear range detection limit was estimated to be 10 µl to 70 µl. The calculated sensitivity is 17.49 µA µM-1 cm-2 and the limit of detection (LOD) and limit of quantification (LOQ) were found to be 3.0nM and 10.0nM. The fabricated cost-effective G/In2O3 electroactive biosensor was effectively employed to regulate FAV in urine and tablet samples with adequate repossession. The proposed sensor offers good selectivity, sensitivity and long-term stability.
A statistical risk assessment model of hazard chain induced by landslide and its application to the Baige landslide
Authors: Yang Feng-Yuan , Zhuo Li , Xiao Ming-Li , Xie Hong-Qiang , Liu Huai-Zhong , He Jiang-Da 
Published: November 2022

Abstract
Landslides are usually caused by geological processes such as rainstorms or earthquakes and may have a massive impact on human production and life. The hazard chain of landslide-river blockage-outburst flood is the most common hazard chain caused by landslides. A database based on existing landslide cases was established in this paper to investigate the assessment formulas of river blocking risk, dam stability, and peak flood discharge after the dam break. A risk assessment model of the landslide-river blocking-breaching hazard chain was established with the vulnerability downstream. The case of the Baige landslide verifies the applicability of the model. This model can be used in a landslide-prone area to predict whether it will form a relatively massive river blockage after the landslide occurs, whether the landslide dam formed by river blockage will breach in a short time, as well as the impact of the outburst flood on the downstream area.
A numerical approach based on n-dimensional fractional Müntz-Legendre polynomials for solving fractional-order cohomological equations with variable coefficients
Authors: Nezhad Akbar Dehghan , Zeabadi Mina Moghaddam 
Published: November 2022

Abstract
This research presents a numerical approach to obtain the approximate solution of the n-dimensional co-homological equations of fractional order in continuous-time dynamical systems. For this purpose, the n-dimensional fractional Müntz-Legendre polynomials (or n-DFMLPs) are introduced. The operational matrix of the fractional Riemann-Liouville derivative is constructed by employing n-DFMLPs. Our method transforms the cohomological equation of fractional order into a system of algebraic equations. Therefore, the solution of that system of algebraic equations is the solution of the associated cohomological equation. The existence of solution for FCEs by means of classical fixed point theory is proven. Also, the stability of the approximate solution of the cohomological equation is checked with respect to a small perturbation of the function g(x). The error bound and convergence analysis of the applied method under the L2-norm is discussed. Some examples are considered and discussed to confirm the efficiency and accuracy of our method. The actual error is computed and compared for different values of k as the number of n-DFMLPs.
Mathematics Subject Classification (2020): 37C05, 35R11
Patterns of attentional biases in children and emotional symptoms during the COVID-19 pandemic: a two-wave longitudinal study
Authors: Zhang Qiaochu 
Published: November 2022

Abstract

Background. It is unknown how the patterns of negative and positive attentional biases in children predict fear of COVID-19, anxiety, and depression during the COVID-19 pandemic. The study identified profiles of negative and positive attentional biases in children and examined their association with emotional symptoms during the COVID-19 pandemic.
Method. 322 children were involved in a two-wave longitudinal study. Children completed the self-report assessment of fear of COVID-19, anxiety, depression, and attention to positive and negative information in classrooms. After six months, they completed the second assessment of fear of COVID-19, anxiety, and depression in classrooms.
Results. Higher fear of COVID-19 was related to higher anxiety and depression throughout the assessment period. Three profiles of negative and positive attentional biases were revealed in children. Children with a “moderate positive and high negative attentional biases” profile had significantly higher fear of COVID-19 pandemic, anxiety, and depression than children with a “high positive and moderate negative attentional biases” profile. Children with a “low positive and negative attentional biases” profile were not significantly different in fear of COVID-19, anxiety, and depression than those with the other two profiles.
Conclusions. Patterns of negative and positive attentional biases were related to emotional symptoms during the COVID-19 pandemic. It might be important to consider children's overall patterns of negative and positive attentional biases to identify children at risk of higher emotional symptoms.
SC 2 R: Strategic cooperative cluster-based routing for Internet of Underwater Things
Authors: Gupta Swati , Singh Niraj Pratap 
Published: November 2022

AbstractOceanographic data gathering, pollution monitoring, offshore exploration, catastrophe avoidance, aided navigation, and tactical surveillance are all expected to benefit from the Internet of Underwater Things (IoUT). However, the viability of various applications in underwater scenarios is possible only if the routing among the sensor nodes employed is strategically optimized. This paper proposes Strategic Cooperative Routing for IoUT (SC2R) that employs a cooperative node for data collection from each Cluster-Head (CH). CH selection is done through the energy and distance parameters. The cooperative nodes are positioned on water surface, other nodes being placed in the underwater terrain. This cooperative routing helps in the data collection for the time-critical scenario as it avoids multi-hop communication among the sensor nodes underwater. Due to decreased number of hops of communication, the delay in data transmission is reduced. The simulation results illustrate the efficacy of the proposed routing technique in comparison to competitive algorithms. The proposed protocol outperforms state of art routing protocols in terms of Network Lifetime and End to End Delay (EED).