bluuebunny/crossref_metadata_2025_split huggingface dataset:
How To Fall Into A New Routine: Factors Influencing The Implementation Of An Admission And Discharge Program In Hospitals And General Practices
Authors: Forstner Johanna , Litke Nicola , Weis Aline , Straßner Cornelia , Szecsenyi Joachim , Wensing Michel 
Published: February 2022

Abstract
Introduction: The VESPEERA programme is a multifaceted programme to enhance information transfers across the whole process from admission, stay and discharge from the hospital to general practice. It was implemented in 7 hospitals and 72 general practices in Southern Germany. Intervention fidelity was heterogeneous, but overall low. A process evaluation aimed at identifying factors associated with the implementation of the VESPEERA programme.Methods: This was a qualitative study, using semi-structured interviews, in a purposeful sample of health workers in hospitals and general practices in the VESPEERA programme. Qualitative framework analysis using the Consolidated Framework for Implementation Research was performed and revealed the topic of previous and new routines to be protruding. Then, content analysis was used and an inductive set of four stages to describe the process of staying in a previous or falling into a new routine by implementing the VESPEERA programme was developed.Results: Thirty-six interviews were conducted with 17 participants from general practices and 19 participants from hospitals. The interviewees were in different stages of the implementation process at the time of the interviews. The four stages are as following: Stage 1, “Previous routine and tension for change”, describes the initial setting in which VESPEERA was to be implemented and the factors leading to the decision to participate. In stage 2, “Adoption of the VESPEERA programme”, factors that influenced whether individuals decided to employ the innovation is described. Stage 3 comprises ”Determinants for falling into and staying in the new VESPEERA-routine”, and finally, in stage 4, the participants reflect on the success of the implementation. Conclusions: The individuals and organisations participating in the VESPEERA programme were in different stages of a from the previous to the new routine. These stages were characterised by different determinants to implementation. Emerging topics were related to organisational characteristics, such as size of the organisation, hierarchy, and leadership. Developing a new routine was identified as beneficial for implementation the innovation.Trial Registration: DRKS00015183 on DRKS / Universal Trial Number (UTN): U1111-1218-0992
Ascorbic acid and Proline alleviat the adverse effects of salinity stress in Duranta erecta L. var. Variegata plants
Authors: Hamoud Magda , El-Banna Sabah , Mostafa Gehan Gaber , Mohamed yehia , Hassan Makka 
Published: February 2022

Abstract
The present study was carried out at the Faculty of Desert Agriculture and Environmental in Fouka, Matrouh University (Matrouh governorate) Egypt, during the two successive seasons of 2019/2020 and 2020/2021. This investigatin aimed to study the effects of ascorbic acid (0.0, 100, 200 ppm) and proline (0.0, 40, 80 ppm) on alleviating the adverse effects of salinity stress at 0.0, 2000, 4000, 6000 ppm seawater in Duranta erecta L. var. Variegata plants. The experiment was designed in a split-plot design in random completely block design (RCBD) with three replications. In this experiment, the main plot was salt water levels and the sub-plot was spraying by proline and ascorbic acid treatments. The results showed that treated plants with proline at 80 ppm and ascorbic acid at 200ppm enhanced plant height, leaves dry weight, leaf green color degree (SPAD units), plant survival percentage, stomata measurement, potassium of leaves and antioxidant such as superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), cytochrome b5, cytochrome P450 (CYP450), amidopyrine N-demethylase and aniline 4-hydroxylase activity. Moreover, treatments deacreased thiobarbituric acid reactive substances (TBARS), sodium and chlorid. Finaly, proline and ascorbic acid alleviate the negative effects of salinity and increase Duranta plant growth and plant survival percentage by inhancing the antioxidant enzymes, potassium cations and photosynthetic system, decresing DNA breakage %, harmful ions eg:sodium and chloride.
Harmonisation of in-silico next-generation sequencing based methods for diagnostics and surveillance
Authors: Nunez-Garcia J. , AbuOun M. , Storey N. , Brouwer M. , Delgado-Blas J.F , Mo S. S. , Ellaby N. , Veldman K. T. , Haenni M. , Châtre P. , Madec J. Y. , Hammerl J. A. , Serna C. , Getino M. , LaRagione R. , Naas T. , Telke A. A. , Glaser P. , Sunde M. , Gonzalez-Zorn B. , Ellington M. J. , Anjum M. 
Published: February 2022

Abstract
Improvements in cost and speed of next generation sequencing (NGS) have provided a new pathway for delivering disease diagnosis, molecular typing, and detection of antimicrobial resistance (AMR). Numerous published methods and protocols exist, but a lack of harmonisation has hampered meaningful comparisons between results produced by different methods/protocols vital for global genomic diagnostics and surveillance. As an exemplar, this study evaluated the sensitivity and specificity of five well-established in-silico AMR detection software, produced from whole genome sequencing a panel of 438 Escherichia coli. No significant differences in software performance were observed, suggesting that efforts to harmonise AMR predictions from sequence data should focus on: 1) establishing universal minimum levels of performance thresholds (e.g. a control isolate panel, minimum sensitivity/specificity thresholds); 2) standardising AMR gene identifiers in reference databases and gene nomenclature; 3) producing consistent genotype/phenotype correlations. The study also revealed limitations of in-silico technology on detecting resistance to certain antimicrobials due to lack of specific fine-tuning options in bioinformatics tool or a lack of representation of resistance mechanisms in the reference database. Lastly, we noted user friendliness of tools was also an important consideration. Therefore, our recommendations are timely for widespread standardisation of bioinformatics for genomic diagnostics and surveillance globally.
B blood group is related to decreased risk of incidence and severity of coronary artery disease in elderly adults with hypertension
Authors: Song Yanbin , Cai Gaojun 
Published: February 2022

Abstract
Background Although ABO blood groups have been associated with cardiovascular disease, little is known about whether ABO blood groups contribute to the risk of incidence and severity of coronary artery disease (CAD) in elderly adult with hypertension. Here, the study was carried out to explore the association.Methods 793 hypertensive patients aged 60 years or older of 2095 cases who consecutively underwent primary coronary angiography were retrospectively included. They were divided into CAD and non-CAD groups. Demographic and clinical characteristics, ABO blood groups and other biochemical parameters of both groups were collected and compared. Further evaluation was performed to determine the impact of ABO blood groups on CAD severity using Gensini score and the number of significantly diseased vessels. Logistic regression model was constructed to identify the association of ABO blood groups with CAD. Results There was substantial difference in distribution of ABO blood groups in elderly and hypertensive adults with and without CAD (p=0.022). Hypertensive patients with CAD had significantly lower distribution of B blood group than those without CAD (p=0.008). Compared to those with non-B blood groups, hypertensive elderly with B blood group tended to have significantly lower concentrations of TC, LDL-C and Apo B, and statistically lower number of significantly stenosed vessels. B blood group was found to be an independently protective factor for CAD in elderly with hypertension.Conclusions B blood group was significantly associated with a decreased risk of CAD and was correlated with severity of coronary stenosis in elderly with hypertension.
Phylogenetic Conservation of Microbial Responses to Elevated Ozone
Authors: Yu Zhengsheng , Gao Qun , Guo Xue , Peng Jinlong , Qi Qi , Gao Mengying , Mo Cehui , Feng Zhaozhong , Wong Minghung , Yang Yunfeng , Li Hui 
Published: February 2022

Abstract
Background: Global tropospheric ozone has doubled due to emissions from fossil fuel and biomass burning, and thus reduces plant primary productivity and crop yields. In contrast, little is known about how elevated tropospheric ozone affects soil microbial communities in the cropland ecosystem and whether such effects are sensitive to nitrogen (N) supply. Here, we examined the responses of bacterial and fungal communities in maize soils to elevated ozone (+60 ppb ozone) across different levels of N fertilization (+60, +120, and +240 kg N ha-1yr-1). Results: Proteobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Elusimicrobia, and Planctomycetes were altered by N fertilization or elevated ozone (P < 0.05). No interactive effects between N fertilization and elevated ozone were observed for bacterial and fungal communities (P > 0.1). While bacterial responses to N fertilization were phylogenetically conserved, bacterial and fungal responses to elevated ozone were phylogenetically conserved, showing universal homogeneous selection (homogeneous environmental conditions leading to more similar community structures). The ozone-responsive phyla were generally correlated (P < 0.05) with plant biomass, plant carbon (C) uptake, and soil dissolved organic C. Conclusions: Our study highlighted that microbial response to elevated ozone displayed a phylogenetic clustering pattern, suggesting that response strategies to elevated ozone stress may be a phylogenetically conserved ecological trait.
Serum, urine and fecal metabolomic characterization of a zinc- deficient experimental rat model
Authors: Wang Peng , Liu Zhipeng , Zhang Yingfeng , Wang Maoqing , Li Ying 
Published: February 2022

Abstract
Background
Zinc serves as a cofactor for over 10% of the human proteome, including transcription factors and enzymes, and maintains protein structure and function. This provides a strong evidence regarding the involvement of zinc in metabolic processes. Zinc deficiency is one of the most common nutrient deficiencies worldwide. Our objective was to comprehensively determine how zinc deficiency alters serum, urine, and fecal metabolic profiles.
Methods
Five-week-old growing Sprague–Dawley rats (n = 30) were housed individually in stainless steel metabolic cages, with ad libitum access to food and water. Isoenergetic and isonitrogenous diets were procured commercially. Serum, urine, and fecal samples were collected from zinc-sufficient (n = 15) and zinc-deficient (n = 15) rats after feeding a diet with an adequate amount of zinc (30 mg/kg) or a zinc-deficient diet (10 mg/kg), respectively, for four weeks. Samples from these three sources were metabolically profiled using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry.
Results
A total of 33, 18, and 16 differential metabolites were identified in the serum, urine, and feces, respectively, between the zinc-sufficient and zinc-deficient rats (P < 0.05). The integrated analysis of altered serum, urine, and fecal metabolites revealed altered amino acid profiles in serum and urine, decreased acylcarnitine levels in serum and urine, increased bile acid excretion in feces, bile acid accumulation in serum, increased lysophospholipidcholine levels, and changes in arachidonic acid catabolites in the serum and feces of zinc-deficient rats.
Conclusions
In summary, our results confirm previous studies that altered metabolism of essential amino acids, glucose, lipid, and bile acid in rats in response to zinc deficiency. For the first time, we reported which improved the effect of zinc deficiency on metabolic homeostasis.
Barriers and Facilitators to Implementing Sustainable School Meals in Swedish High School — A Qualitative Study with Students and Meal Staff
Authors: Myers Charlotte , Colombo Patricia Eustachio , Elinder Liselotte Schäfer , Andermo Susanne 
Published: February 2022

Abstract
Background: Adoption of more sustainable diets is necessary to preserve human and planetary health. The reach of the school meal system makes it an ideal setting to focus a food-related intervention towards more sustainable meals. The motivation to shift to more sustainable eating is increasing in the younger generation. However, there is currently limited insight into why and how these preferences develop and can be promoted. Aim: To explore barriers and facilitators to implementing sustainable school meals in the Swedish high school setting. Methods: This study employed a mixed inductive-deductive qualitative design. Interviews with high school students (n=16) and school kitchen staff (n=3) were conducted and transcribed verbatim. Data was first analysed inductively using qualitative content analysis. Emerging barriers and facilitators were consequently mapped deductively onto the domains of the Consolidated Framework of Implementation Research (CFIR).Results: Ten barriers and seven facilitators to implementing more sustainable high school meals emerged. These belonged to two domains of the CFIR: individual characteristics of students and kitchen staff and inner setting factors such as school meal environment and relationships between stakeholders. Barriers included a low desirability toward vegetarian meals as well as lack of knowledge on the nutritional value of plant-based proteins among students. A barrier for the meal staff was that they were less inclined to prepare more plant-based meals which were perceived as less acceptable among students. Facilitators included a common interest in change towards sustainable meals and a good relationship between students and meal staff. Also, kitchen staff perceived that they have the knowledge and resources necessary to implement change. Conclusion: Prioritizing students’ food preferences as well as their knowledge about food and sustainability is likely to be key, but possible not enough, to achieve successful implementation of sustainable school meals. Co-creation with consumers and providers could provide an important opportunity to reaching higher acceptability of new meals and thus avoiding food waste.
CircSLC8A1 inhibits sunitinib resistance in renal cell carcinoma by promoting the ubiquitination and degradation of FUS
Authors: Wang Linhui , Wang Jie , Gan Xinxin , Chen Qiong , Liu Bing , Zheng Sujuan , Bao Yi , Wang Anbang , Yan Rui , Bao Yewei , Gong Wenliang , Wang Yuexiang , Yang Fu 
Published: February 2022

Abstract
Background: Circular RNAs play an important role in many biological processes, including tumor occurrence and development. Targeted drug such as sunitinib is currently the first-line therapy for the treatment of metastatic renal cell carcinoma. Drug resistance is an important problem faced by clinicians. Currently, the role and underlying molecular mechanisms of circular RNAs in targeted drug resistance for renal cell carcinoma remain unknown.Methods: We generated the sunitinib-resistant renal cell carcinoma (RCC) cell lines Sun-7R and Sun-AR. Then, we used RNA sequencing (RNA-Seq) to identify the circular RNA related to targeted drug resistance in RCC, cSLC8A1. qRT-PCR was used to detect the expression of circRNAs，mRNAs in human tissues and cells. The actinomycin D experiment, RNase R digestion experiment, and fluorescence in situ hybridization (FISH) experiment were performed to verify the circularity characteristics of cSLC8A1. CCK-8 experiment and plate clone experiment were performed to verify the reactivity of RCC cells to sunitinib. The effects of circSLC8A1 on RCC cells were explored by transfecting with knockdown and over-expressed lentivirus in vitro and in vivo. Western-blot and immunohistochemical experiment were used to analyze the protein expression level of FUS.Results: We verified that cSLC8A1 was expressed at low levels in sunitinib-resistant tissues and cell lines，and cSLC8A1 expression was associated with response to sunitinib in RCC patients . Overexpression of cSLC8A1 inhibited cell proliferation and migration, thereby increasing the sensitivity of renal cell carcinoma cells to sunitinib. We found a novel function and mechanism of circRNAs that cSLC8A1 promotes the ubiquitination and degradation of the FUS protein and then mediates the drug resistance process.Conclusions: cSLC8A1 inhibits resistance by promoting the ubiquitination and degradation of the FUS protein. cSLC8A1 plays an important role in drug resistance and is expected to become a target for reversing sunitinib resistance in RCC.
Encouraging Young Pediatric Surgeons and Evaluation of the Effectiveness of a Pediatric Endosurgery Workshop by Self-assessment and an Objective Skill Validation System: The Japan-Russia Medical Cooperation Project
Authors: Ogawa Katsuhiro , Ieiri Satoshi , Watanabe Toshihiko , Bitoh Yuko , Uchida Hiroo , Yamataka Atsuyuki , Ohno Yasuharu , Ohta Masayuki , Inomata Masafumi , Dorofeeva Elena , Podurovskaya Yulia , Yarotskaya Ekaterina , Kitano Seigo 
Published: February 2022

Abstract
Background: Japanese pediatric endosurgery experts conducted a workshop for young pediatric surgeons in Russia in collaboration with Russian expert pediatric surgeons. This study was aimed to develop a contributive workshop program and evaluate its impact on young Russian pediatric surgeons. Methods: A two-day pediatric endosurgery workshop was held in Russia. After conducting a needs assessment survey, Japanese and Russian faculties developed the workshop contents, including pre- and post-workshop skills assessments, lectures and hands-on training. Skills assessments were performed using the objective skill validation system, the “A-Lap Mini”, mimicking intestinal anastomosis. The trainees self-evaluated their knowledge and skills using a five-point scale.Results: Fifteen novice Russian trainees participated and 14 (93.3%) completed the workshop. The completion rate for the suturing task before and after the workshop was 40.0% (6/15) and 85.7% (12/14), respectively. The following five skill evaluation criteria, which were objectively evaluated, improved: performance time changed from 751.6 ± 247.1 s to 780.0 ± 313.3 s (p>0.05), number of full-thickness sutures improved from 1.0 ± 1.41 to 2.64 ± 0.84 (p=0.003), area of wound-opening changed from 0.42 ± 0.83 mm2 to 0.53 ± 1.13 mm2, suture tension improved from 55.48 ± 19.51% to 61.95 ± 23.91% (p>0.05), and maximum air leakage pressure improved from 3.76 ± 2.11 kPa to 8.42 ± 7.68 kPa (p>0.05). Regarding the self-assessed questionnaire administered before and after the workshop, the confidence in endosurgery skills significantly improved as follows: forceps manipulation ability improved from 2.7 to 3.7 (p<0.05), and suturing performance improved from 2.5 to 3.6 (p<0.05). The usefulness of the workshop for clinical surgery was scored at 4.3.Conclusions: Quantitative skill evaluation with an automatic feedback function was useful for endosurgery training. Delivering feedback concerning the assessment results to the trainee helps them to determine the specific training requirements needed for clinical endosurgery.
Evaluation of the Impact of the COVID-19 Lockdown on BMI in Children and Adolescents with or Without Obesity
Authors: Maggio Albane Bertha Rosa , Gal-Duding Claudine , Martin Xavier Eric , Weber Catherine Chamay 
Published: February 2022

Abstract
Background
In Switzerland, from March 15th to May 11th 2020, schools and most shops were closed nationwide due to the COVID-19-related lockdown. This cessation of activities may have impacted weight gain in children and adolescents. The aims of our study were to evaluate the effects of the COVID-19 lockdown on the BMI of children and adolescents in treatment for obesity, and to compare its evolution to that of the previous year at the same time, as well as to that of normal-weight children.
Methods
This retrospective study gathered demographic and anthropometric data from subjects aged 2–18 years both with normal weight and with obesity, who attended our hospital clinics at four time points: before and after the lockdown period in 2020, and at the same times of the year in 2019. We used paired t-tests to assess weight, BMI and BMI z-score changes, linear and standard multiple regressions, independent Student’s t-tests or Chi-square tests to compare groups, and Pearson correlation coefficient when appropriate.
Results
Forty-seven children with obesity and 20 normal-weight subjects had complete data for the 4 visits. The mean BMI increased in both groups during the lockdown (obese: +0.96 ± 1.5 vs. control: +0.51 ± 0.5), however the increase was significantly more important in the subjects with obesity compared to the same period in 2019 (2019: +0.33 ± 1.0; mean difference between 2019 and 2020: +0.63 ± 2.0 p = 0.034).
Conclusion
The COVID-19 lockdown had a negative impact on the BMI of youth with obesity. Interestingly we observed extreme changes in this population, which was not the case in normal-weight children. Therefore, families with a child with obesity must be actively supported during these stressful and obesogenic periods of confinement.
Prevalence and Factors Associated with High Risk for Coronary Events in the Brazilian Population: Evidence from Two Household Surveys
Authors: Silva Pedro Gilson , Câmara Daniel Cardoso Portela , Simões Taynãna César , Santos Juliano dos , Ferreira Aline Alves , Pierin Angela Maria Geraldo , Meira Karina Cardoso 
Published: February 2022

Abstract
Circulatory system diseases are the main causes of death and disability in the world. The aim of this study was to analyze changes in prevalence and factors associated with high risk for coronary events (HRC) over time in the adult Brazilian population. As analytical tools, proportion differences tests and generalized linear models were used considering complex survey samplings, namely NHS 2013 and NHS 2019, to test changes in prevalence of HRC over time and estimate adjusted prevalence ratios for variables sociodemographic, health conditions, and habits and lifestyles. Risk stratification was performed based on the first phase of the I Brazilian Guidelines for Cardiovascular Prevention. There was an increase in the proportion of HRC between surveys (10.05 vs 12.11, p<0.001). After adjusting the multiple model, there was a higher prevalence of HRC in residents of the Center-South region of Brazil, with advancing age, male, with regular, poor or very poor health conditions, smoking history, health conditions underlying factors such as hypertension and high cholesterol, overweight/obesity, and abuse of alcohol and ultra-processed foods. The results of this study point to the need for more effective measures, with a longitudinal and comprehensive approach to prevent and care for affected individuals, with the aim of preventing new acute coronary events in the next ten years.