bluuebunny/crossref_metadata_2025_split huggingface dataset:
The more the better? How and when can high-performance work systems fuel the proactive fire
Authors: Lin Chiou-Shiu , Xiao Ran , Huang Pei-Chi , Huang Liang-Chih , Jin Ming 
Published: October 2022

AbstractDrawing on the job demands-resources model and conservation of resources theory, this study investigates how and when the high-performance work systems (HPWS) influence proactive workforce. Using the data obtained from 204 supervisor-employee dyads in China, we developed and tested a moderated mediation model in which leader-member exchange (LMX) moderates the positive relationship between HPWS and proactive behavior via work engagement. Our results demonstrated that the association between HPWS and proactivity was fully mediated by work engagement. We also found that the indirect effect of HPWS on proactivity was significantly weaker among employees with high-quality LMX. Theoretical and practical implications, as well as directions for future research, are then discussed.
Irremediability in psychiatric euthanasia: examining the objective standard
Authors: Nicolini Marie E. , Jardas EJ , Zarate Carlos A. , Gastmans Chris , Kim Scott Y. H. 
Published: October 2022

AbstractBackgroundIrremediability is a key requirement for euthanasia and assisted suicide for psychiatric disorders (psychiatric EAS). Countries like the Netherlands and Belgium ask clinicians to assess irremediability in light of the patient's diagnosis and prognosis and ‘according to current medical understanding’. Clarifying the relevance of a default objective standard for irremediability when applied to psychiatric EAS is crucial for solid policymaking. Yet so far, a thorough examination of this standard is lacking.MethodsUsing treatment-resistant depression (TRD) as a test case, through a scoping review in PubMed, we analyzed the state-of-the-art evidence for whether clinicians can accurately predict individual long-term outcome and single out irremediable cases, by examining the following questions: (1) What is the definition of TRD; (2) What are group-level long-term outcomes of TRD; and (3) Can clinicians make accurate individual outcome predictions in TRD?ResultsA uniform definition of TRD is lacking, with over 150 existing definitions, mostly focused on psychopharmacological research. Available yet limited studies about long-term outcomes indicate that a majority of patients with long-term TRD show significant improvement over time. Finally, evidence about individual predictions in TRD using precision medicine is growing, but methodological shortcomings and varying predictive accuracies pose important challenges for its implementation in clinical practice.ConclusionOur findings support the claim that, as per available evidence, clinicians cannot accurately predict long-term chances of recovery in a particular patient with TRD. This means that the objective standard for irremediability cannot be met, with implications for policy and practice of psychiatric EAS.
Cooperative collision avoidance in multirobot systems using fuzzy rules and velocity obstacles
Authors: Tang Wenbing , Zhou Yuan , Zhang Tianwei , Liu Yang , Liu Jing , Ding Zuohua 
Published: October 2022

AbstractCollision avoidance is critical in multirobot systems. Most of the current methods for collision avoidance either require high computation costs (e.g., velocity obstacles and mathematical optimization) or cannot always provide safety guarantees (e.g., learning-based methods). Moreover, they cannot deal with uncertain sensing data and linguistic requirements (e.g., the speed of a robot should not be large when it is near to other robots). Hence, to guarantee real-time collision avoidance and deal with linguistic requirements, a distributed and hybrid motion planning method, named Fuzzy-VO, is proposed for multirobot systems. It contains two basic components: fuzzy rules, which can deal with linguistic requirements and compute motion efficiently, and velocity obstacles (VOs), which can generate collision-free motion effectively. The Fuzzy-VO applies an intruder selection method to mitigate the exponential increase of the number of fuzzy rules. In detail, at any time instant, a robot checks the robots that it may collide with and retrieves the most dangerous robot in each sector based on the predicted collision time; then, the robot generates its velocity in real-time via fuzzy inference and VO-based fine-tuning. At each time instant, a robot only needs to retrieve its neighbors’ current positions and velocities, so the method is fully distributed. Extensive simulations with a different number of robots are carried out to compare the performance of Fuzzy-VO with the conventional fuzzy rule method and the VO-based method from different aspects. The results show that: Compared with the conventional fuzzy rule method, the average success rate of the proposed method can be increased by 306.5%; compared with the VO-based method, the average one-step decision time is reduced by 740.9%.
On stochastic ordering among extreme shock models
Authors: Manesh Sirous Fathi , Izadi Muhyiddin , Khaledi Baha-Eldin 
Published: October 2022

AbstractIn the usual shock models, the shocks arrive from a single source. Bozbulut and Eryilmaz [(2020). Generalized extreme shock models and their applications. Communications in Statistics – Simulation and Computation49(1): 110–120] introduced two types of extreme shock models when the shocks arrive from one of $m\geq 1$ possible sources. In Model 1, the shocks arrive from different sources over time. In Model 2, initially, the shocks randomly come from one of $m$ sources, and shocks continue to arrive from the same source. In this paper, we prove that the lifetime of Model 1 is less than Model 2 in the usual stochastic ordering. We further show that if the inter-arrival times of shocks have increasing failure rate distributions, then the usual stochastic ordering can be generalized to the hazard rate ordering. We study the stochastic behavior of the lifetime of Model 2 with respect to the severity of shocks using the notion of majorization. We apply the new stochastic ordering results to show that the age replacement policy under Model 1 is more costly than Model 2.
An outbreak of Cryptosporidium parvum linked to pasteurised milk from a vending machine in England: a descriptive study, March 2021
Authors: Gopfert Anya , Chalmers Rachel M. , Whittingham Sarah , Wilson Laura , van Hove Maria , Ferraro Claire F. , Robinson Guy , Young Nick , Nozad Bayad 
Published: October 2022

Abstract
We describe the investigations and management of a Cryptosporidium parvum outbreak of linked to consumption of pasteurised milk from a vending machine. Multiple locus variable number of tandem repeats analysis was newly used, confirming that C. parvum detected in human cases was indistinguishable from that in a calf on the farm. This strengthened the evidence for milk from an on-farm vending machine as the source of the outbreak because of post-pasteurisation contamination. Bacteriological indicators of post-pasteurisation contamination persisted after the initial hygiene improvement notice. We propose that on-farm milk vending machines may represent an emerging public health risk.
Incorporating Network Scale River Bathymetry to Improve Characterization of Fluvial Processes in Flood Modeling
Authors: Dey Sayan , Saksena Siddharth , Winter Danielle , Merwade Venkatesh , McMillan Sara 
Published: October 2022

AbstractSeveral studies have focused on the importance of river bathymetry (channel geometry) in hydrodynamic routing along individual reaches. However, its effect on other watershed processes such as infiltration and surface water (SW)‐groundwater (GW) interactions has not been explored across large river networks. Surface and sbsurface processes are interdependent, therefore, errors due to inaccurate representation of one watershed process can cascade across other hydraulic or hydrologic processes. This study hypothesizes that accurate bathymetric representation is not only essential for simulating channel hydrodynamics but also affects subsurface processes by impacting SW‐GW interactions. Moreover, quantifying the effect of bathymetry on surface and subsurface hydrological processes across a river network can facilitate an improved understanding of how bathymetric characteristics affect these processes across large spatial domains. The study tests this hypothesis by developing physically based distributed models capable of bidirectional coupling (SW‐GW) with four configurations with progressively reduced levels of bathymetric representation. A comparison of hydrologic and hydrodynamic outputs shows that changes in channel geometry across the four configurations has a considerable effect on infiltration, lateral seepage, and location of water table across the entire river network. For example, when using bathymetry with inaccurate channel conveyance capacity but accurate channel depth, peak lateral seepage rate exhibited 58% error. The results from this study provide insights into the level of bathymetric detail required for accurately simulating flooding‐related physical processes while also highlighting potential issues with ignoring bathymetry across lower order streams such as spurious backwater flow, inaccurate water table elevations, and incorrect inundation extents.
The universal stem cell
Authors: Quesenberry Peter J. , Wen Sicheng , Goldberg Laura R. , Dooner Mark S. 
Published: October 2022

AbstractCurrent dogma is that there exists a hematopoietic pluripotent stem cell, resident in the marrow, which is quiescent, but with tremendous proliferative and differentiative potential. Furthermore, the hematopoietic system is essentially hierarchical with progressive differentiation from the pluripotent stem cells to different classes of hematopoietic cells. However, results summarized here indicate that the marrow pluripotent hematopoietic stem cell is actively cycling and thus continually changing phenotype. As it progresses through cell cycle differentiation potential changes as illustrated by sequential changes in surface expression of B220 and GR-1 epitopes. Further data indicated that the potential of purified hematopoietic stem cells extends to multiple other non-hematopoietic cells. It appears that marrow stem cells will give rise to epithelial pulmonary cells at certain points in cell cycle. Thus, it appears that the marrow “hematopoietic” stem cell is also a stem cell for other non-hematopoietic tissues. These observations give rise to the concept of a universal stem cell. The marrow stem cell is not limited to hematopoiesis and its differentiation potential continually changes as it transits cell cycle. Thus, there is a universal stem cell in the marrow which alters its differentiation potential as it progresses through cell cycle. This potential is expressed when it resides in tissues compatible with its differentiation potential, at a particular point in cell cycle transit, or when it interacts with vesicles from that tissue.
Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage
Authors: Steffens Reinhardt Luiza , Zhang Xiajie , Groen Kira , Morten Brianna C. , De Iuliis Geoffry N. , Braithwaite Antony W. , Bourdon Jean-Christophe , Avery-Kiejda Kelly A. 
Published: October 2022

AbstractOur previous studies have shown that p53 isoform expression is altered in breast cancer and related to prognosis. In particular, a high ∆40p53:p53α ratio is associated with worse disease-free survival. In this manuscript, the influence of altered Δ40p53 and p53α levels on the response to standard of care DNA-damaging agents used in breast cancer treatment was investigated in vitro. Our results revealed that a high Δ40p53:p53α ratio causes cells to respond differently to doxorubicin and cisplatin treatments. Δ40p53 overexpression significantly impairs the cells’ sensitivity to doxorubicin through reducing apoptosis and DNA damage, whereas Δ40p53 knockdown has the opposite effect. Further, a high Δ40p53:p53α ratio inhibited the differential expression of several genes following doxorubicin and promoted DNA repair, impairing the cells’ canonical response. Overall, our results suggest that the response of breast cancer cells to standard of care DNA-damaging therapies is dependent on the expression of p53 isoforms, which may contribute to outcomes in breast cancer.
Integrated proteogenomic characterization across major histological types of pituitary neuroendocrine tumors
Authors: Zhang Fan , Zhang Qilin , Zhu Jiajun , Yao Boyuan , Ma Chi , Qiao Nidan , He Shiman , Ye Zhao , Wang Yunzhi , Han Rui , Feng Jinwen , Wang Yongfei , Qin Zhaoyu , Ma Zengyi , Li Kai , Zhang Yichao , Tian Sha , Chen Zhengyuan , Tan Subei , Wu Yue , Ran Peng , Wang Ye , Ding Chen , Zhao Yao 
Published: October 2022

AbstractPituitary neuroendocrine tumor (PitNET) is one of the most common intracranial tumors. Due to its extensive tumor heterogeneity and the lack of high-quality tissues for biomarker discovery, the causative molecular mechanisms are far from being fully defined. Therefore, more studies are needed to improve the current clinicopathological classification system, and advanced treatment strategies such as targeted therapy and immunotherapy are yet to be explored. Here, we performed the largest integrative genomics, transcriptomics, proteomics, and phosphoproteomics analysis reported to date for a cohort of 200 PitNET patients. Genomics data indicate that GNAS copy number gain can serve as a reliable diagnostic marker for hyperproliferation of the PIT1 lineage. Proteomics-based classification of PitNETs identified 7 clusters, among which, tumors overexpressing epithelial-mesenchymal transition (EMT) markers clustered into a more invasive subgroup. Further analysis identified potential therapeutic targets, including CDK6, TWIST1, EGFR, and VEGFR2, for different clusters. Immune subtyping to explore the potential for application of immunotherapy in PitNET identified an association between alterations in the JAK1-STAT1-PDL1 axis and immune exhaustion, and between changes in the JAK3-STAT6-FOS/JUN axis and immune infiltration. These identified molecular markers and alternations in various clusters/subtypes were further confirmed in an independent cohort of 750 PitNET patients. This proteogenomic analysis across traditional histological boundaries improves our current understanding of PitNET pathophysiology and suggests novel therapeutic targets and strategies.
Towards the engineering of a photon-only two-stroke rotary molecular motor
Authors: Filatov Michael , Paolino Marco , Pierron Robin , Cappelli Andrea , Giorgi Gianluca , Léonard Jérémie , Huix-Rotllant Miquel , Ferré Nicolas , Yang Xuchun , Kaliakin Danil , Blanco-González Alejandro , Olivucci Massimo 
Published: October 2022

AbstractThe rational engineering of photoresponsive materials, e.g., light-driven molecular motors, is a challenging task. Here, we use structure-related design rules to prepare a prototype molecular rotary motor capable of completing an entire revolution using, exclusively, the sequential absorption of two photons; i.e., a photon-only two-stroke motor. The mechanism of rotation is then characterised using a combination of non-adiabatic dynamics simulations and transient absorption spectroscopy measurements. The results show that the rotor moiety rotates axially relative to the stator and produces, within a few picoseconds at ambient T, an intermediate with the same helicity as the starting structure. We discuss how such properties, that include a 0.25 quantum efficiency, can help overcome the operational limitations of the classical overcrowded alkene designs.
On the fluorescence enhancement of arch neuronal optogenetic reporters
Authors: Barneschi Leonardo , Marsili Emanuele , Pedraza-González Laura , Padula Daniele , De Vico Luca , Kaliakin Danil , Blanco-González Alejandro , Ferré Nicolas , Huix-Rotllant Miquel , Filatov Michael , Olivucci Massimo 
Published: October 2022

AbstractThe lack of a theory capable of connecting the amino acid sequence of a light-absorbing protein with its fluorescence brightness is hampering the development of tools for understanding neuronal communications. Here we demonstrate that a theory can be established by constructing quantum chemical models of a set of Archaerhodopsin reporters in their electronically excited state. We found that the experimentally observed increase in fluorescence quantum yield is proportional to the computed decrease in energy difference between the fluorescent state and a nearby photoisomerization channel leading to an exotic diradical of the protein chromophore. This finding will ultimately support the development of technologies for searching novel fluorescent rhodopsin variants and unveil electrostatic changes that make light emission brighter and brighter.
Library adaptors with integrated reference controls improve the accuracy and reliability of nanopore sequencing
Authors: Gunter Helen M. , Youlten Scott E. , Madala Bindu Swapna , Reis Andre L. M. , Stevanovski Igor , Wong Ted , Kummerfield Sarah K. , Deveson Ira W. , Santini Nadia S. , Marcellin Esteban , Mercer Tim R. 
Published: October 2022

AbstractLibrary adaptors are short oligonucleotides that are attached to RNA and DNA samples in preparation for next-generation sequencing (NGS). Adaptors can also include additional functional elements, such as sample indexes and unique molecular identifiers, to improve library analysis. Here, we describe Control Library Adaptors, termed CAPTORs, that measure the accuracy and reliability of NGS. CAPTORs can be integrated within the library preparation of RNA and DNA samples, and their encoded information is retrieved during sequencing. We show how CAPTORs can measure the accuracy of nanopore sequencing, evaluate the quantitative performance of metagenomic and RNA sequencing, and improve normalisation between samples. CAPTORs can also be customised for clinical diagnoses, correcting systematic sequencing errors and improving the diagnosis of pathogenic BRCA1/2 variants in breast cancer. CAPTORs are a simple and effective method to increase the accuracy and reliability of NGS, enabling comparisons between samples, reagents and laboratories, and supporting the use of nanopore sequencing for clinical diagnosis.
Oxygen mediated oxidative couplings of flavones in alkaline water
Authors: Yang Xin , Lim Sophie Hui Min , Lin Jiachen , Wu Jie , Tang Haidi , Zhao Fengyue , Liu Fang , Sun Chenghua , Shi Xiangcheng , Kuang Yulong , Toy Joanne Yi Hui , Du Ke , Zhang Yuannian , Wang Xiang , Sun Mingtai , Song Zhixuan , Wang Tian , Wu Ji’en , Houk K. N. , Huang Dejian 
Published: October 2022

AbstractCatalyzedoxidative C-C bond coupling reactions play an important role in the chemical synthesis of complex natural products of medicinal importance. However, the poor functional group tolerance renders them unfit for the synthesis of naturally occurring polyphenolic flavones. We find that molecular oxygen in alkaline water acts as a hydrogen atom acceptor and oxidant incatalyst-free(without added catalyst) oxidative coupling of luteolin and other flavones. By this facile method, we achieve the synthesis of a small collection of flavone dimers and trimers including naturally occurring dicranolomin, philonotisflavone, dehydrohegoflavone, distichumtriluteolin, and cyclodistichumtriluteolin. Mechanistic studies using both experimental and computational chemistry uncover the underlying reasons for optimal pH, oxygen availability, and counter-cations that define the success of the reaction. We expect our reaction opens up a green and sustainable way to synthesize flavonoid dimers and oligomers using the readily available monomeric flavonoids isolated from biomass and exploiting their use for health care products and treatment of diseases.
Light activation of Orange Carotenoid Protein reveals bicycle-pedal single-bond isomerization
Authors: Chukhutsina Volha U. , Baxter James M. , Fadini Alisia , Morgan Rhodri M. , Pope Matthew A. , Maghlaoui Karim , Orr Christian M. , Wagner Armin , van Thor Jasper J. 
Published: October 2022

AbstractOrange Carotenoid protein (OCP) is the only known photoreceptor which uses carotenoid for its activation. It is found exclusively in cyanobacteria, where it functions to control light-harvesting of the photosynthetic machinery. However, the photochemical reactions and structural dynamics of this unique photosensing process are not yet resolved. We present time-resolved crystal structures at second-to-minute delays under bright illumination, capturing the early photoproduct and structures of the subsequent reaction intermediates. The first stable photoproduct shows concerted isomerization of C9’-C8’ and C7’-C6’ single bonds in the bicycle-pedal (s-BP) manner and structural changes in the N-terminal domain with minute timescale kinetics. These are followed by a thermally-driven recovery of the s-BP isomer to the dark state carotenoid configuration. Structural changes propagate to the C-terminal domain, resulting, at later time, in the H-bond rupture of the carotenoid keto group with protein residues. Solution FTIR and UV/Vis spectroscopy support the single bond isomerization of the carotenoid in the s-BP manner and subsequent thermal structural reactions as the basis of OCP photoreception.
Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo
Authors: Ventura Guilherme , Amiri Aboutaleb , Thiagarajan Raghavan , Tolonen Mari , Doostmohammadi Amin , Sedzinski Jakub 
Published: October 2022

AbstractDuring embryonic development, regeneration, and homeostasis, cells have to migrate and physically integrate into the target tissues where they ultimately execute their function. While much is known about the biochemical pathways driving cell migration in vivo, we are only beginning to understand the mechanical interplay between migrating cells and their surrounding tissue. Here, we reveal that multiciliated cell precursors in the Xenopus embryo use filopodia to pull at the vertices of the overlying epithelial sheet. This pulling is effectively used to sense vertex stiffness and identify the preferred positions for cell integration into the tissue. Notably, we find that pulling forces equip multiciliated cells with the ability to remodel the epithelial junctions of the neighboring cells, enabling them to generate a permissive environment that facilitates integration. Our findings reveal the intricate physical crosstalk at the cell-tissue interface and uncover previously unknown functions for mechanical forces in orchestrating cell integration.
Van der Waals lattice-induced colossal magnetoresistance in Cr2Ge2Te6 thin flakes
Authors: Zhu Wenxuan , Song Cheng , Han Lei , Guo Tingwen , Bai Hua , Pan Feng 
Published: October 2022

AbstractRecent discovery of two-dimensional (2D) magnets with van der Waals (vdW) gapped layered structure prospers the fundamental research of magnetism and advances the miniaturization of spintronics. Due to their unique lattice anisotropy, their band structure has the potential to be dramatically modulated by the spin configuration even in thin flakes, which is still unexplored. Here, we demonstrate the vdW lattice-induced spin modulation of band structure in thin flakes of vdW semiconductor Cr2Ge2Te6 (CGT) through the measurement of magnetoresistance (MR). The significant anisotropic lattice constructed by the interlayer vdW force and intralayer covalent bond induces anisotropic spin-orbit field, resulting in the spin orientation-dependent band splitting. Consequently, giant variation of resistance is induced between the magnetization aligned along in-plane and out-of-plane directions. Based on this, a colossal MR beyond 1000% was realized in lateral nonlocal devices with CGT acting as a magneto switch. Our finding provides a unique feature for the vdW magnets and would advance its applications in spintronics.