bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regeneration
Authors: He Jianbo , Zhou Yang , Qian Chuanfang , Wang Danyang , Yang Zhuolin , Huang Zhuofu , Sun Junhui , Ni Rui , Yang Qifen , Chen Jingying , Luo Lingfei 
Published: March 2022

AbstractIn cases of extensive liver injury, biliary epithelial cells (BECs) dedifferentiate into bipotential progenitor cells (BPPCs), then redifferentiate into hepatocytes and BECs to accomplish liver regeneration. Whether epigenetic regulations, particularly DNA methylation maintenance enzymes, play a role in this biliary-mediated liver regeneration remains unknown. Here we show that in response to extensive hepatocyte damages, expression of dnmt1 is upregulated in BECs to methylate DNA at the p53 locus, which represses p53 transcription, and in turn, derepresses mTORC1 signaling to activate BEC dedifferentiation. After BEC dedifferentiation and BPPC formation, DNA methylation at the p53 locus maintains in BPPCs to continue blocking p53 transcription, which derepresses Bmp signaling to induce BPPC redifferentiation. Thus, this study reveals promotive roles and mechanisms of DNA methylation at the p53 locus in both dedifferentiation and redifferentiation stages of biliary-mediated liver regeneration, implicating DNA methylation and p53 as potential targets to stimulate regeneration after extensive liver injury.
Virucidal efficacy of laundry sanitizers against SARS-CoV-2 and other coronaviruses and influenza viruses
Authors: Khalid Ijaz M. , Nims Raymond W. , McKinney Julie , Gerba Charles P. 
Published: March 2022

AbstractThe clothes laundering process affords numerous opportunities for dissemination of infectious virus from contaminated clothing to appliance surfaces and other household surfaces and eventually to launderer’s hands. We have explored the efficacy of laundry sanitizers for inactivating coronaviruses and influenza viruses. Virucidal efficacy was tested using standardized suspension inactivation methods (EN 14476) or hard-surface inactivation methods (ASTM E1053-20) against SARS-CoV-2, human coronavirus 229E (HCoV 229E), influenza A virus (2009-H1N1 A/Mexico), or influenza B virus (B/Hong Kong). Efficacy was measured in terms of log10 reduction in infectious virus titer, after 15 min contact time (suspension studies) or 5 min contact time (hard surface studies) at 20 ± 1 °C. In liquid suspension studies, laundry sanitizers containing p-chloro-m-xylenol (PCMX) or quaternary ammonium compounds (QAC) caused complete inactivation (≥ 4 log10) of HCoV 229E and SARS-CoV-2 within 15 min contact time at 20 ± 1 °C. In hard surface studies, complete inactivation (≥ 4 log10) of each coronavirus or influenza virus, including SARS-CoV-2, was observed following a 5-min contact time at 20 ± 1 °C. Respiratory viruses may remain infectious on clothing/fabrics and environmental surfaces for hours to days. The use of a laundry sanitizer containing microbicidal actives may afford mitigation of the risk of contamination of surfaces during handling of the laundry and washing appliances (i.e., washer/dryer or basin), adjacent surfaces, the waste water stream, and the hands of individuals handling clothes contaminated with SARS-CoV-2, influenza viruses, or other emerging enveloped viruses.
Recognizing megatsunamis in Mediterranean deep sea sediments based on the massive deposits of the 365 CE Crete event
Authors: Polonia A. , Nelson C. H. , Vaiani S. C. , Colizza E. , Gasparotto G. , Giorgetti G. , Bonetti C. , Gasperini L. 
Published: March 2022

AbstractThe Mediterranean Sea hosts two subduction systems along the convergent Africa-Eurasia plate boundary that have produced strong ground shaking and generated tsunamis. Based on historical descriptions and sedimentary records, one of these events, in 365 CE, impacted a broad geographical area, including tsunami evidence for distances of 700–800 km from the source event, qualifying it as a ‘megatsunami’. Understanding how megatsunamis are produced, and where they are more likely, requires a better understanding of the different secondary processes linked to these events such as massive slope failures, multiple turbidity current generation, and basin seiching. Our sedimentary records from an extensive collection of cores located in distal and disconnected basins, identify turbidites which are analyzed using granulometry, elemental (XRF), micropaleontological, and geochemical data in order to reconstruct their coastal or marine source. The results show that the 365 CE basin floor sediments are a mixture of inner shelf and slope materials. The tsunami wave produced multiple far-field slope failures that resulted in stacked basal turbidites. It also caused transport of continent-derived organic carbon and deposition over basal turbidites and into isolated basins of the deep ocean. The composition of sediment in isolated basins suggests their deposition by large-scale sheet like flows similar to what has been caused by the Tohoku earthquake associated tsunamis. This is significant for rectifying and resolving where risk is greatest and how cross-basin tsunamis are generated. Based on these results, estimates of the underlying deposits from the same locations were interpreted as possible older megatsunamis.
Predictors of permanent pacemaker implantation after sinus conversion of cavotricuspid isthmus-dependent atrial flutter
Authors: Kim Juwon , Lee Sung Ho , Kim Hye Ree , Chung Tae-Wan , Choi Ji-Hoon , Kim Ju Youn , Park Seung-Jung , On Young Keun , Kim June Soo , Park Kyoung-Min 
Published: March 2022

AbstractIt is unclear which factors are associated with progressive sinus node dysfunction after cavotricuspid isthmus (CTI)-dependent atrial flutter (AFL) ablation. We sought to evaluate the incidence and predictors for permanent pacemaker (PPM) implantation after CTI-dependent AFL ablation. Between January 2011 and June 2021, 353 patients underwent CTI-dependent AFL ablation were studied. During a median follow-up of 31.6 months, 30 patients (8.5%) received PPM implantation, 24 for sick sinus syndrome and 6 for atrioventricular block. In multivariable model, prior atrial fibrillation (AF) (HR 3.570; 95% CI 1.034–12.325; P = 0.044), lowest previous sinus heart rate (HR 0.942; 95% CI 0.898–0.988; P = 0.015), and left atrial volume index (LAVI) (HR 1.067; 95% CI 1.024–1.112; P = 0.002) were independently associated with PPM implantation after CTI-dependent AFL ablation. The best cut-off points for predicting PPM implantation were 60.1 ml/m2 for LAVI and 46 beats per minute for lowest previous sinus heart rate. Among the patients discharged without PPM implantation after ablation, sinus pause over three seconds at AFL termination during ablation was an independent predictor of PPM implantation (HR 17.841; 95% CI 4.626–68.807; P < 0.001). Physicians should be aware of the possibility of PPM implantation during follow-up after AFL ablation, especially in patients with the relevant risk factors.
Activated endothelial cells induce a distinct type of astrocytic reactivity
Authors: Taylor Xavier , Cisternas Pablo , Jury Nur , Martinez Pablo , Huang Xiaoqing , You Yanwen , Redding-Ochoa Javier , Vidal Ruben , Zhang Jie , Troncoso Juan , Lasagna-Reeves Cristian A. 
Published: March 2022

AbstractReactive astrogliosis is a universal response of astrocytes to abnormal events and injuries. Studies have shown that proinflammatory microglia can polarize astrocytes (designated A1 astrocytes) toward a neurotoxic phenotype characterized by increased Complement Component 3 (C3) expression. It is still unclear if inflammatory stimuli from other cell types may also be capable of inducing a subset of C3+neurotoxic astrocytes. Here, we show that a subtype of C3+neurotoxic astrocytes is induced by activated endothelial cells that is distinct from astrocytes activated by microglia. Furthermore, we show that endothelial-induced astrocytes have upregulated expression of A1 astrocytic genes and exhibit a distinctive extracellular matrix remodeling profile. Finally, we demonstrate that endothelial-induced astrocytes are Decorin-positive and are associated with vascular amyloid deposits but not parenchymal amyloid plaques in mouse models and AD/CAA patients. These findings demonstrate the existence of potentially extensive and subtle functional diversity of C3+-reactive astrocytes.
Flow behavior and microstructure evolution of Al-3.65Cu-0.98Li (wt%) alloy during hot deformation
Authors: Lin Hai-tao , Long Shuai , Wu Dao-xiang , Li Shi-shan , Zhou Yu-ting , Wang Shu-yan , Dai Qing-wei 
Published: March 2022

Abstract
The phenomenological constitutive model, processing map and microstructure evolution of Al-3.65Cu-0.98Li (wt%) alloy were studied by means of isothermal compression tests conducted on a Gleeble-3500 isothermal simulator in the temperature range of 350 °C−500 °C and strain rate range of 0.01 s−1–10 s−1 and EBSD. The strain compensated Arrhenius model describes the flow curves accurately with a relative error of 0.9898 and an average absolute relative error of 4.70%. The plastic capability was characterized by strain rate sensitivity index, and it has the positive relation with temperature and negative relation with strain rate. The processing map was constructed, the instable deformation window and optimal hot working window of this alloy was identified to be 350 ∼ 455 °C & 0.37 ∼ 10s−1 and 440 ∼ 500 °C & 0.01 ∼ 0.368s−1, respectively. Moreover, the dynamic recrystallization occurs more violently at lower strain rate, and most portion were transformed into substructure with increasing strain rate, which was nearly vanished at the strain rate of 10 s−1. The geometric necessary dislocation distributions under different temperatures and strain rates were analyzed. More uniform distributed dislocation cell structures were observed at high temperature with low strain rate conditions and intensive dislocation and more pileups occurs at the contrary conditions.
Effect of suspension parameters towards the fabrication of BSCCO films by electrophoretic deposition
Authors: Lv Fangxia , Li Jianqiang , Zhang Xu , Zhao Zipeng , Wang Wei 
Published: March 2022

Abstract
Superconductor Bi-Sr-Ca-Cu-O (BSCCO) thin films were fabricated on silver by electrophoretic deposition (EPD) method. By investigating the effects of solvents and I2 additives on the properties of suspension prepared of BSCCO powder, the particle charging mechanism is analyzed. Studying the effect of different solvents on the structure of BSCCO powder, isopropanol is the best dispersion solvent. Through the optimization of the I2 concentration, the results show that the optimal concentration of I2 is 0.08 mg ml−1. The processing parameters critically influencing the morphology of BSCCO films are identified and optimized to obtain uniform films with high packing density of BSCCO particles. After heated at 820 °C for 20 h, the smoothest surface morphology, high c-axis orientation, and highest critical temperature(Tc) of 82 K are obtained from the film prepared with optimized suspension.
Spontaneous coronary artery dissection, fibromuscular dysplasia, and biventricular stress cardiomyopathy: a case report
Authors: Blazak Penni L , Holland David J , Basso Thomas , Martin Josh 
Published: March 2022

Abstract

Background
Spontaneous coronary artery dissection (SCAD) is an important cause of acute coronary syndrome and is associated with fibromuscular dysplasia (FMD). The diagnosis of stress cardiomyopathy in patients with SCAD and FMD is uncommon, though an important consideration given the shared risk profile. Complications of severe left ventricular (LV) dysfunction associated with stress cardiomyopathy, such as LV thrombus, complicate the management of SCAD where anticoagulation is controversial in the context of SCAD-associated intramural haematoma.

Case summary
A 65-year-old female presented with non-ST elevation myocardial infarction with a recent diagnosis of hypertension but no other traditional cardiovascular risk factors. There was, however, a family history of early cardiac death from myocardial infarction affecting her mother. Echocardiography demonstrated severe biventricular dysfunction with circumferential akinesis of the mid to apical segments. Coronary angiography demonstrated type 2A SCAD involving the first diagonal artery. Cardiac magnetic resonance imaging (MRI) confirmed a diagnosis of stress cardiomyopathy with biventricular involvement, complicated by LV apical thrombus and a focal region of myocardial infarction. Vascular imaging confirmed the presence of FMD. Guideline-directed heart failure therapy in addition to clopidogrel and rivaroxaban was prescribed. Follow-up contrast echocardiography at six-weeks confirmed resolution of LV dysfunction and resolution of the LV thrombus with no adverse events.

Discussion
The dual diagnosis of SCAD and stress cardiomyopathy is uncommon. Cardiac MRI was useful for confirming the diagnosis of stress cardiomyopathy and the presence of LV thrombus, where anticoagulation may complicate the management of intramural haematoma in patients with concomitant SCAD and FMD.
Pulmonary vascular disease in pulmonary hypertension due to left heart disease: pathophysiologic implications
Authors: Omote Kazunori , Sorimachi Hidemi , Obokata Masaru , Reddy Yogesh N V , Verbrugge Frederik H , Omar Massar , DuBrock Hilary M , Redfield Margaret M , Borlaug Barry A 
Published: March 2022

Abstract

Aims
Pulmonary hypertension (PH) and pulmonary vascular disease (PVD) are common and associated with adverse outcomes in left heart disease (LHD). This study sought to characterize the pathophysiology of PVD across the spectrum of PH in LHD.

Methods and results
Patients with PH-LHD [mean pulmonary artery (PA) pressure &gt;20 mmHg and PA wedge pressure (PAWP) ≥15 mmHg] and controls free of PH or LHD underwent invasive haemodynamic exercise testing with simultaneous echocardiography, expired air and blood gas analysis, and lung ultrasound in a prospective study. Patients with PH-LHD were divided into isolated post-capillary PH (IpcPH) and PVD [combined post- and pre-capillary PH (CpcPH)] based upon pulmonary vascular resistance (PVR &lt;3.0 or ≥3.0 WU). As compared with controls (n = 69) and IpcPH-LHD (n = 55), participants with CpcPH-LHD (n = 40) displayed poorer left atrial function and more severe right ventricular (RV) dysfunction at rest. With exercise, patients with CpcPH-LHD displayed similar PAWP to IpcPH-LHD, but more severe RV–PA uncoupling, greater ventricular interaction, and more severe impairments in cardiac output, O2 delivery, and peak O2 consumption. Despite higher PVR, participants with CpcPH developed more severe lung congestion compared with both IpcPH-LHD and controls, which was associated lower arterial O2 tension, reduced alveolar ventilation, decreased pulmonary O2 diffusion, and greater ventilation-perfusion mismatch.

Conclusions
Pulmonary vascular disease in LHD is associated with a distinct pathophysiologic signature marked by greater exercise-induced lung congestion, arterial hypoxaemia, RV–PA uncoupling, ventricular interdependence, and impairment in O2 delivery, impairing aerobic capacity. Further study is required to identify novel treatments targeting the pulmonary vasculature in PH-LHD.
Colistin-resistant Enterobacterales among veterinary healthcare workers and in the Dutch population
Authors: Dierikx C. M. , Meijs A. P. , Hengeveld P. D. , van der Klis F. R. M. , van Vliet J. , Gijsbers E. F. , Rozwandowicz M. , van Hoek A. H. A. M. , Hendrickx A. P. A. , Hordijk J. , Van Duijkeren E. 
Published: March 2022

Abstract

Objectives
Plasmid-mediated colistin resistance can be transferred from animals to humans. We investigated the prevalence of carriage of mcr-mediated colistin-resistant Escherichia coli and Klebsiella pneumoniae (ColR-E/K) in veterinary healthcare workers and in the general population in the Netherlands.

Methods
Two cross-sectional population studies were performed: one among veterinary healthcare workers and one in the general population. Participants sent in a faecal sample and filled in a questionnaire. Samples were analysed using selective enrichment and culture. Mobile colistin resistance genes (mcr) were detected by PCR and ColR-E/K were sequenced using Illumina and Nanopore technologies.

Results
The prevalence of mcr-mediated ColR-E/K was 0.2% (1/482, 95% CI 0.04%–1.17%) among veterinary personnel and 0.8% (5/660, 95% CI 0.3%–1.8%) in the population sample. mcr-1 was found in E. coli from four persons, mcr-8 in K. pneumoniae from one person and another person carried both mcr-1 and mcr-8 in a K. pneumoniae isolate. mcr-1 was found on different plasmid types (IncX4, IncI1 and IncI2), while mcr-8 was found on IncF plasmids only.

Conclusions
mcr-mediated ColR-E/K resistance was uncommon in both populations. Professional contact with animals does not increase the chance of carriage of these bacteria in the Netherlands at present. mcr-8 was found for the first time in the Netherlands. Surveillance of colistin resistance and its underlying mechanisms in humans, livestock and food is important in order to identify emerging trends in time.
Characterization of the AARTFAAC-12 aperture array: radio source counts at 42 and 61 MHz
Authors: Shulevski A , Franzen T M O , Williams W L , Vernstrom T , Gehlot B K , Kuiack M , Wijers R A M J 
Published: March 2022

ABSTRACT
Dense aperture arrays provide key benefits in modern astrophysical research. They are flexible, employing cheap receivers, while relying on the ever more sophisticated compute back end to deal with the complexities of signal processing required for optimal use. Their advantage is that they offer very large fields of view and are readily scalable to any size, all other things being equal. Since they represent ‘software telescopes’, the science cases these arrays can be applied to are quite broad. Here, we describe the calibration and performance of the AARTFAAC-12 instrument, which is composed of the twelve centrally located stations of the LOFAR array. We go into the details of the data acquisition and pre-processing, we describe the newly developed calibration pipeline as well as the noise properties of the resulting images and present radio source counts at 41.7 MHz and 61 MHz. We find that AARTFAAC-12 is confusion limited at 0.9 Jy/PSF at 61 MHz with a PSF size of 17 × 11 arcmin and that the normalized source counts agree with the scaled VLSSr and 6C survey counts. The median spectral index of the sources between the two frequencies we observed at is -0.78. Further, we have used the derived source counts to estimate any excess cosmic radio background, and we do not find evidence for it at our observing frequencies compared to published literature values.
The Fix is in? Parliamentary Scrutiny of Appointments of the Heads of Brazilian Regulatory Agencies
Authors: Lopes André Vaz , Vieira Diego Mota 
Published: March 2022

Abstract
This manuscript analyses the parliamentary scrutiny of the presidential appointments to the direction of independent Brazilian Regulatory Agencies from an in-depth case study of the heads of the Brazilian Health Regulatory Agency—ANVISA, between 1999 and 2021. In Brazil, the Senate has the final decision on these appointments, exercising a dual function: parliamentary control over presidential patronage and selecting future heads of agencies. The objective of this article was to explain how the parliament exercises these functions and interferes in the appointment process. The results indicate that, although the formal process of parliamentary scrutiny seems like a game of marked cards, where it is already known in advance that the result will be approval, the Parliament plays an anticipatory role of presidential decisions, interfering in the timing of appointments and the possibility of influence over the choice of nominees.
Aminoflavone upregulates putative tumor suppressor miR-125b-2-3p to inhibit luminal A breast cancer stem cell-like properties
Authors: Mavingire Nicole , Campbell Petreena , Liu Tiantian , Wooten Jonathan , Khan Salma , Chen Xin , Matthews Jason , Wang Charles , Brantley Eileen 
Published: March 2022

Abstract
Metastatic breast cancer is incurable and often due to breast cancer stem cell (CSC)-mediated self-renewal. We previously determined that aryl hydrocarbon receptor (AhR) agonist aminoflavone (AF) inhibits the expression of CSC biomarker α6-integrin (ITGA6) to disrupt the formation of luminal (hormone receptor-positive) mammospheres (3D breast cancer spheroids). In this study, we performed miRNA-sequencing analysis of luminal A MCF-7 mammospheres treated with AF to gain further insight into the mechanism of AF-mediated anti-cancer and anti-breast CSC activity. AF significantly induced the expression of over 70 miRNAs including miR125b-2-3p, a predicted stemness gene regulator. AF-mediated miR125b-2-3p induction was validated in MCF-7 mammospheres and cells. miR125b-2-3p levels were low in breast cancer tissues irrespective of subtype compared to normal breast tissues. While miR125b-2-3p levels were low in MCF-7 cells, they were much lower in AHR100 cells (MCF-7 cells made unresponsive to AhR agonists). The miR125b-2-3p mimic decreased, while the antagomiR125b-2-3p increased the expression of stemness genes ITGA6 and SOX2 in MCF-7 cells. In MCF-7 mammospheres, the miR125b-2-3p mimic decreased only ITGA6 expression though the antagomiR125b-2-3p increased ITGA6, SOX2 and MYC expression. AntagomiR125b-2-3p reversed AF-mediated suppression of ITGA6. The miR125b-2-3p mimic decreased proliferation, migration, and mammosphere formation while the antagomiR125b-2-3p increased proliferation and mammosphere formation in MCF-7 cells. The miR125b-2-3p mimic also inhibited proliferation, mammosphere formation, and migration in AHR100 cells. AF induced AhR- and miR125b2-3p-dependent anti-proliferation, anti-migration, and mammosphere disruption in MCF-7 cells. Our findings suggest miR125b-2-3p is a tumor suppressor and AF upregulates miR125b-2-3p to disrupt mammospheres via mechanisms that rely at least partially on AhR in luminal A breast cancer cells.
Social mobility and biological aging among older adults in the United States
Authors: Graf Gloria Huei-Jong , Zhang Yalu , Domingue Benjamin W , Harris Kathleen Mullan , Kothari Meeraj , Kwon Dayoon , Muennig Peter , Belsky Daniel W 
Published: March 2022

Abstract
Lower socioeconomic status is associated with faster biological aging, the gradual and progressive decline in system integrity that accumulates with advancing age. Efforts to promote upward social mobility may, therefore, extend healthy lifespan. However, recent studies suggest that upward mobility may also have biological costs related to the stresses of crossing social boundaries. We tested associations of life-course social mobility with biological aging using data from participants in the 2016 Health and Retirement Study (HRS) Venous Blood Study who provided blood-chemistry (n = 9,255) and/or DNA methylation (DNAm) data (n = 3,976). We quantified social mobility from childhood to later-life using data on childhood family characteristics, educational attainment, and wealth accumulation. We quantified biological aging using 3 DNAm “clocks” and 3 blood-chemistry algorithms. We observed substantial social mobility among study participants. Those who achieved upward mobility exhibited less-advanced and slower biological aging. Associations of upward mobility with less-advanced and slower aging were consistent for blood-chemistry and DNAm measures of biological aging, and were similar for men and women and for Black and White Americans (Pearson-r effect-sizes ∼0.2 for blood-chemistry measures and the DNAm GrimAge clock and DunedinPoAm pace-of-aging measures; effect-sizes were smaller for the DNAm PhenoAge clock). Analysis restricted to educational mobility suggested differential effects by racial identity; mediating links between educational mobility and healthy aging may be disrupted by structural racism. In contrast, mobility producing accumulation of wealth appeared to benefit White and Black Americans equally, suggesting economic intervention to reduce wealth inequality may have potential to heal disparities in healthy aging.