bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective cholinergic stimulation of the medial septum-diagonal band of Broca via DREADDs improves spatial learning in healthy rats
Authors: Missault Stephan , De Waegenaere Sam , Kosten Lauren , Van der Linden Annemie , Verhoye Marleen , Keliris Georgios A. 
Published: August 2022

AbstractThe septohippocampal pathway plays an important role in learning and memory. It projects from the medial septum-vertical limb of the diagonal band of Broca (MSDB) to the hippocampus and provides the latter with its main cholinergic innervation. To assess the importance of cholinergic selectivity and timing of MSDB stimulation in modulating learning and memory, we directly compared the effects of several MSDB stimulation strategies in healthy rats. We evaluated the effects of DREADD-mediated selective cholinergic neuronal MSDB stimulation and nonselective neuronal MSDB stimulation on spatial learning and memory in the appetitive radial arm maze and on resting-state brain networks using resting-state functional MRI. DREADDs were activated with the novel DREADD agonist J60. Selective cholinergic MSDB stimulation during – but not after – radial arm maze training improved spatial learning compared with J60-treated sham rats and had no effect on working memory or reversal learning. J60-treated sham rats had a worse working memory than saline-treated sham rats during the reversal phase of the radial arm maze task, suggesting an adverse effect of chronic use of J60. Nonselective MSDB stimulation during training resulted in a loss of appetite and exclusion from the radial arm maze training. Acute selective cholinergic and nonselective MSDB stimulation induced decreased functional connectivity (FC) in the default mode-like network. In addition, acute nonselective MSDB stimulation resulted in increased intrahippocampal FC, while selective cholinergic MSDB stimulation led to globally increased FC with the nucleus accumbens. While the combined effect of radial arm maze learning and the necessary chronic food restriction with or without chronic MSDB stimulation had no observable effect on resting-state networks, chronic food restriction alone globally increased FC in the brain.
Untethered Muscle Tracking Using Magnetomicrometry
Authors: Taylor Cameron R. , Yeon Seong Ho , Clark William H. , Clarrissimeaux Ellen G. , O’Donnell Mary Kate , Roberts Thomas J. , Herr Hugh M. 
Published: August 2022

AbstractMuscle tissue drives nearly all movement in the animal kingdom, providing power, mobility, and dexterity. Technologies for measuring muscle tissue motion, such as sonomicrometry, fluoromicrometry, and ultrasound, have significantly advanced our understanding of biomechanics. Yet, the field lacks the ability to monitor muscle tissue motion for animal behavior outside the lab. Towards addressing this issue, we previously introduced magnetomicrometry, a method that uses magnetic beads to wirelessly monitor muscle tissue length changes, and we validated magnetomicrometry via tightly-controlled in situ testing. In this study we validate the accuracy of magnetomicrometry against fluoromicrometry during untethered running in an in vivo turkey model. We demonstrate real-time muscle tissue length tracking of the freely-moving turkeys executing various motor activities, including ramp ascent and descent, vertical ascent and descent, and free roaming movement. Given the demonstrated capacity of magnetomicrometry to track muscle movement in untethered animals, we feel that this technique will enable new scientific explorations and an improved understanding of muscle function.
Epigenetic profiling and incidence of disrupted development point to gastrulation as aging ground zero in Xenopus laevis
Authors: Zhang Bohan , Tarkhov Andrei E. , Ratzan Wil , Ying Kejun , Moqri Mahdi , Poganik Jesse R. , Barre Benjamin , Trapp Alexandre , Zoller Joseph A. , Haghani Amin , Horvath Steve , Peshkin Leonid , Gladyshev Vadim N. 
Published: August 2022

AbstractRecent studies suggest the existence of a natural rejuvenation event during early embryonic development of mice, followed by epigenetic aging. Here, by profiling embryonic DNA methylation in the African clawed frog, Xenopus laevis, we found that the epigenetic entropy basepoint maps to the gastrulation stage of embryogenesis and corresponds to a rapid increase in embryo transcript abundance. We further developed a frog aging clock, revealing that this species epigenetically ages. Application of this clock to developmental stages identified a decrease in epigenetic age during early embryogenesis, with the minimal age reached around gastrulation. By examining individual developmental trajectories of 6,457 embryos, we found that this stage is also accompanied by a higher incidence of disrupted development. Taken together, our data point to gastrulation as a critical stage for aging and natural rejuvenation, characterized by the lowest epigenetic age, increased mortality, nadir of DNA methylation entropy and rapid increase in embryo transcript abundance, defining aging ground zero as the basepoint where rejuvenation ends and the aging process begins.
Single cell, whole embryo phenotyping of pleiotropic disorders of mammalian development
Authors: Huang Xingfan , Henck Jana , Qiu Chengxiang , Sreenivasan Varun K. A. , Balachandran Saranya , Behncke Rose , Chan Wing-Lee , Despang Alexandra , Dickel Diane E. , Haag Natja , Hägerling Rene , Hansmeier Nils , Hennig Friederike , Marshall Cooper , Rajderkar Sudha , Ringel Alessa , Robson Michael , Saunders Lauren , Srivatsan Sanjay R. , Ulferts Sascha , Wittler Lars , Zhu Yiwen , Kalscheuer Vera M. , Ibrahim Daniel , Kurth Ingo , Kornak Uwe , Beier David R. , Visel Axel , Pennacchio Len A. , Trapnell Cole , Cao Junyue , Shendure Jay , Spielmann Malte 
Published: August 2022

AbstractMouse models are a critical tool for studying human diseases, particularly developmental disorders, as well as for advancing our general understanding of mammalian biology. However, it has long been suspected that conventional approaches for phenotyping are insufficiently sensitive to detect subtle defects throughout the developing mouse. Here we set out to establish single cell RNA sequencing (sc-RNA-seq) of the whole embryo as a scalable platform for the systematic molecular and cellular phenotyping of mouse genetic models. We applied combinatorial indexing-based sc-RNA-seq to profile 101 embryos of 26 genotypes at embryonic stage E13.5, altogether profiling gene expression in over 1.6M nuclei. The 26 genotypes include 22 mouse mutants representing a range of anticipated severities, from established multisystem disorders to deletions of individual enhancers, as well as the 4 wildtype backgrounds on which these mutants reside. We developed and applied several analytical frameworks for detecting differences in composition and/or gene expression across 52 cell types or trajectories. Some mutants exhibited changes in dozens of trajectories (e.g., the pleiotropic consequences of altering the Sox9 regulatory landscape) whereas others showed phenotypes affecting specific subsets of cells. We also identify differences between widely used wildtype strains, compare phenotyping of gain vs. loss of function mutants, and characterise deletions of topological associating domain (TAD) boundaries. Intriguingly, even among these 22 mutants, some changes are shared by heretofore unrelated models, suggesting that developmental pleiotropy might be “decomposable” through further scaling of this approach. Overall, our findings show how single cell profiling of whole embryos can enable the systematic molecular and cellular phenotypic characterization of mouse mutants with unprecedented breadth and resolution.
Phenotype-driven identification of drug targets for post-COVID-19 anosmia
Authors: Catterson Victoria M. , Sanchez Yolanda , Musso Gabriel 
Published: August 2022

AbstractAnosmia (loss of sense of smell) is one symptom of COVID-19 which can linger long after acute infection has passed, with major impact on quality of life. Given the number of people impacted by COVID-19-related anosmia, there is an urgent need to identify effective therapeutics in a faster fashion than using traditional drug discovery and development methods. We used our knowledge graph, the Phenograph, to navigate from phenotypes to genes to drug targets, to rapidly find druggable targets associated with anosmia. This process shortlisted six targets: NRP1, SCN9A, EGR1, VEGFB, PRKCE, and FGFR1. Neuropilin-1 (NRP1) is under active study for its involvement in SARS-CoV-2 infection. Importantly, there is no direct link between anosmia and NRP1 in our knowledge graph; the relationship was inferred through the graph structure. Based on this external validation, we derived hypotheses for the involvement of the remaining five targets in COVID-19-related anosmia, and the mechanism of action desired in a drug candidate to correct the hypothesized dysregulation.
Does a corporation’s food recall strategy affect its brand image and consumers’ intention to purchase? A study in the context of cereal recall in China
Authors: Yu Weiping , Cui Fasheng , Han Xiaoyun , Lv Mengjiao 
Published: August 2022

PurposeFood recalls are more potentially harmful than other product recalls. This research aimed to investigate the effect of the recall strategies of food corporations on their brand image and consumers’ purchase intention.Design/methodology/approachThis study adopts a between-subjects experiment of 2 (corporate recall strategy: voluntary recall, mandatory recall) *3 (food recall level (severity): high, medium, low), and recruits 224 consumers involved in cereal product recall in China. The authors inductively examine the effects of voluntary and mandatory recall on consumer perception and behavior intentions in the recall process.FindingsVoluntary recall (vs. mandatory recall) will improve corporate brand responsibility image (vs. brand ability image) and consumers’ purchase intention to focal brand (vs. competitive brand and organic brand). Perceived corporate legitimacy and food safety play a mediating role. The former has a greater positive impact on brand image, and the latter has a more significant favorable influence on purchase intention. Furthermore, recall level has a moderating effect on the association between corporate recall strategy and perceived food safety, but is not significant in the effect of corporate recall strategy on perceived corporate legitimacy.Originality/valuePrevious inconsistent conclusions cannot effectively guide food corporations to manage recall strategies. This paper demonstrates the response mechanism of the recall strategy from the perspective of corporate social responsibility, which is beneficial to food safety crisis management and research.
An inquiry on organic food confusion in the consumer perception: a qualitative perspective
Authors: Dinçer Mustafa Abdül Metin , Arslan Yusuf , Okutan Semih , Dil Esra 
Published: August 2022

PurposeThis study aims to reveal consumer perceptions towards organic food, particularly emphasizing the confusion and vagueness in the public eye.Design/methodology/approachIn total, 21 well-educated and relatively high-income consumers were selected as cases of the study. The knowledge about the organic food concept and organic food preferences were analyzed through the grounded theory analysis. In this analysis, ten categories which are shopping from the close circle, the product information attitude, do it yourself (DIY), true and false facts and dilemmas, information sources, the most preferred organic products, perception of organic food indicator, market place trends, organic food orientation reasons, and attitude toward the organic products/producers were detected as the main issues.FindingsThe authors chose the cases from well-educated people who have a relatively high income, and it is seen that the knowledge levels of consumers are low regarding organic foods. This low-level knowledge becomes apparent in confused and erroneous answers and actions. The confusion over the organic food concept and the institutional image appear as two main findings of the study. The authors named this false fact as organic confusion and dilemmas in this analysis. According to the study’s results, there is a considerable amount of confusion over healthy and natural food contexts.Originality/valueThis study aims to bring a comprehensive delineation to the general perception of the organic food concept in society. And it reveals a detailed feedback for the institutions and companies on how to solve organic food problems such as confusion, institutional image problem, and false facts. Although organic product knowledge is shown as a critical factor in many studies in the literature, the number of studies that examine this situation in depth is insufficient.
Intergovernmental transfers to the Brazilian unified health system and party alignment
Authors: Orellano Veronica Ines Fernandez , Tellini Taís Cardoso 
Published: August 2022

PurposeThis study investigates the impact of political alignment on intergovernmental transfers to the Brazilian unified health system (SUS). The authors analyzed both automatic transfers based on pre-established rules and discretionary transfers, using two criteria of political alignment between mayors and the central government.Design/methodology/approachFor the empirical analysis the authors used regression-discontinuity design (RDD) and the outcomes of close elections between 2001 and 2017.FindingsThe results indicate positive and statistically significant effects of party alignment on the two transfer categories, especially discretionary transfers, but also on transfers based on pre-established rules. The effect of direct party alignment, when mayors and the president are from the same party, is greater than that resulting from coalitions established in municipal and federal elections.Research limitations/implicationsThe positive effect of party alignment was found both in discretionary transfers (those that do not have previously established rules) and some non-discretionary transfers (although they have previously defined regulations). A part of these regulations depends on production capacity and on taking part in programs promoted by the central government, which may produce entropy in the financing system, and a margin to benefit political allies. In the case of the SUS system, it is possible that this entropy is greater in the basic health care category than in the moderate and high complexity one, allowing a higher margin for discretion in transfers allocated to the former. Stricter rules associated to basic health care transfers would be desirable.Practical implicationsIn Brazil, stricter rules and monitoring associated to basic healthcare intergovernmental transfers would be desirable.Social implicationsThe results may inspire some improvement in the mechanisms that govern the distribution of resources to basic healthcare in Brazil, improving social welfare by improving social justice in the distribution of resources to basic healthcare.Originality/valueThe authors does not know any other study about the impact of party alignment on the distribution of intergovernmental transfers to the Brazilian unified health system.
Visualization and mapping of global eHealth research based on keywords
Authors: Nwagwu Williams E. , Onyancha Omwoyo Bosire 
Published: August 2022

Purpose
This paper aims to examine the global pattern of growth and development of eHealth research based on publication headcount, and analysis of the characteristics, of the keywords used by authors and indexers to represent their research content during 1945–2019.

Design/methodology/approach
This study adopted a bibliometric research design and a quantitative approach. The source of the data was Elsevier’s Scopus database. The search query involved multiple search terms because researchers’ choice of keywords varies very significantly. The search for eHealth research publications was limited to conference papers and research articles published before 2020.

Findings
eHealth originated in the late 1990s, but it has become an envelope term for describing much older terms such as telemedicine, and its variants that originated much earlier. The keywords were spread through the 27 Scopus Subject Areas, with medicine (44.04%), engineering (12.84%) and computer science (11.47%) leading, while by Scopus All Science Journal Classification Health Sciences accounted for 55.83% of the keywords. Physical sciences followed with 30.62%. The classifications social sciences and life sciences made only single-digit contributions. eHealth is about meeting health needs, but the work of engineers and computer scientists is very outstanding in achieving this goal.

Originality/value
This study demonstrates that eHealth is an unexplored aspect of health literature and highlights the nature of the accumulated literature in the area. It further demonstrates that eHealth is a multidisciplinary area that is attractive to researchers from all disciplines because of its sensitive focus on health, and therefore requires pooling and integration of human resources and expertise, methods and approaches.
Designing obstacle’s map of an unknown place using autonomous drone navigation and web services
Authors: Tanwar Jitender , Sharma Sanjay Kumar , Mittal Mandeep 
Published: August 2022

PurposeDrones are used in several purposes including examining areas, mapping surroundings and rescue mission operations. During these tasks, they could encounter compound surroundings having multiple obstacles, acute edges and deadlocks. The purpose of this paper is to propose an obstacle dodging technique required to move the drones autonomously and generate the obstacle's map of an unknown place dynamically.Design/methodology/approachTherefore, an obstacle dodging technique is essentially required to move autonomously. The automaton of drones requires complicated vision sensors and a high computing force. During this research, a methodology that uses two basic ultrasonic-oriented proximity sensors placed at the center of the drone and applies neural control using synaptic plasticity for dynamic obstacle avoidance is proposed. The two-neuron intermittent system has been established by neural control. The synaptic plasticity is used to find turning angles from different viewpoints with immediate remembrance, so it helps in decision-making for a drone. Hence, the automaton will be able to travel around and modify its angle of turning for escaping objects during the route in unknown surroundings with narrow junctions and dead ends. Furthermore, wherever an obstacle is detected during the route, the coordinate information is communicated using RESTful Web service to an android app and an obstacle map is generated according to the information sent by the drone. In this research, the drone is successfully designed and automated and an obstacle map using the V-REP simulation environment is generated.FindingsSimulation results show that the drone effectively moves and turns around the obstacles and the experiment of using web services with the drone is also successful in generating the obstacle's map dynamically.Originality/valueThe obstacle map generated by autonomous drone is useful in many applications such as examining fields, mapping surroundings and rescue mission operations.
Human resource management for the resilience of public organizations: a model based on macro-competences
Authors: Macchi Silva Vítor Vasata , Ribeiro Jose Luis Duarte 
Published: August 2022

PurposeThis paper proposes a model composed by macro-competences developed to contribute for the resilience of public organizations.Design/methodology/approachTo propose the model, a literature review in the area of organizations resilience was carried out, and the structural equation modeling (SEM) approach was used. The setting used to validate the proposed model was the Brazilian Federal Institutions of Higher Education.FindingsThe results present five dimensions of action seen as macro-competences that contribute for organizations’ resilience: human resource management, development of individual competencies, risk management, preparedness for response, and responsiveness. The results also point at competences that can be developed in each of those dimensions with a view toward resilience.Practical implicationsCompetences of strategically planning the workforces, of testing the risk hypotheses continuously, and applying the action plans proposed by risk management in response to crises can improve individual and organizational resiliencies.Originality/valueGuided by the proposed model, public organizations will be better prepared to withstand adversities, such as resources cutbacks and staff shortages.
Internal and external networking behaviors and employee outcomes: a test of gender moderating effect
Authors: Wanigasekara Saroja , Ali Muhammad , French Erica Lynn , Baker Marzena 
Published: August 2022

PurposeResearch suggests that engaging in networking behaviors can affect individual work outcomes. However, relatively less is known about how internal versus external networking behaviors influence work outcomes, and whether gender moderates these relationships. Drawing on social capital theory and social role theory, the authors propose a positive relationship between employees' internal and external networking behaviors and their work outcomes (job commitment and career success), and the moderating effect of gender. The authors also explore employee preference in networking.Design/methodology/approachBased on a sequential mixed-method research design with a four-month time lag, Study 1 data on networking behaviors and employee outcomes were collected via a survey of middle managers and their supervisors from 10 private sector organizations in Sri Lanka. Study 2 data were collected via interviews from a sample of those middle managers and their supervisors.FindingsStudy 1 findings indicate a positive relationship between internal networking behaviors and job commitment, and external networking behaviors and career success. The authors also found that internal networking behaviors enhance job commitment. Study 2 findings indicate men and women network differently and benefit differently from that networking but achieve equitable workplace benefits.Originality/valueThis study provides pioneering evidence that internal networking behaviors enhance job commitment among women. It appears that past research did not test the moderating effect of gender for internal versus external networking behaviors separately. Moreover, this study refines the evidence that internal and external networking behaviors differentially impact employee outcomes and explains the processes through a qualitative inquiry.