bluuebunny/crossref_metadata_2025_split huggingface dataset:
The molecular epidemiology, genotyping, and clinical manifestation of prevalent Adenovirus infection during the epidemic keratoconjunctivitis, South of Iran
Authors: Afrasiabi Vahid-Reza , Ghojoghi Rozita , Hosseini Seyed Younes , Sarvari Jamal , Nekooei Fatemeh , Joharinia Negar , Hadian Sahar , Gholami Mohammad , Nejabat Mahmood 
Published: June 2022

Abstract
Purpose
Adenoviral mediated keratoconjunctivitis is among the emergency diseasees of ophthalmology with long-term sequels. The role of adenovirus infection, ocular-related genotypes, and association with ocular symptoms needs to be investigated for epidemiological as well as clinical purposes.
Methods
The affected patients from two close keratoconjunctivitis epidemics were included in the study. The swab samples were taken from patients; total DNA was extracted and then was introduced into an in-house Real-Time PCR reaction. Besides, partial Hexon genes of 11 adenovirus positive samples were amplified and submitted to sanger sequencing. Moreover, they were finally evaluated by phylogenetic analysis.
Results
Of the 153 patients, 92 (60.1%) were males and 47 cases (30.7%) had a history of eye infection in the family or colleagues. A Real-time PCR test of 126 samples (82.4%) was positive for adenovirus, and all eleven cases that underwent sequencing analysis were determined to be serotype 8 (HAdV-D8). Adenovirus infection has a significant relationship with infection among family or colleagues (p = 0.048), membrane formation (p = 0.047), conjunctival bleeding (p = 0.046), and pain.
Conclusions
The results indicated that Adenovirus is the major cause of keratoconjuctivitis and HAdV-D8 was the most common genotype in the area. There were some clinical manifestations associated with Adenovirus infection of the conjunctiva.
The efficacy of computed tomography imaging-based radiotherapy for external auditory canal squamous cell carcinoma
Authors: Osu Naoto , Musha Atsushi , Yumisaki Hikaru , Okada Kohei , Kubo Nobuteru , Okano Naoko , Kawamura Hidemasa , Ohno Tatsuya 
Published: June 2022

Abstract
Background: External auditory canal (EAC) cancer is a rare disease for which there are no adequate evidence-based treatment strategies. Radical radiotherapy is often used as the initial treatment for EAC cancer from the viewpoint of organ preservation. This study aimed to elucidate the efficacy of radiotherapy for squamous cell carcinoma (SCC) of the EAC.Methods: Fifteen consecutive patients with SCC of the EAC who were treated with radical radiotherapy were retrospectively analyzed. The fractional dose was 2.0 or 3.0, and the total dose ranged from 66 to 70 Gy. Three-dimensional conformal radiation therapy was performed on seven patients. Intensity-modulated radiation therapy was performed on eight patients. Patients with T1 disease were treated with radiotherapy alone. Patients with T2-4 disease were treated with chemoradiotherapy.Results: The median follow-up period was 30.4 months. The 3 year local control (LC) rate for all patients was 51%, the disease-free survival (DFS) rate was 44%, and the overall survival (OS) rate was 73%. For T1-3 disease, the 3 year LC rate was 74%, DFS was 62%, and OS was 89%. However, for T4 disease, the 3 year LC rate was 17%, DFS was 17%, and OS was 50%. In a univariate analysis, only the T-category was a significant factor for predicting LC and DFS (p = 0.006 and 0.02, respectively). All local recurrences were within the high-dose irradiated area.Conclusions: The results of this study suggest that chemoradiotherapy is a reasonable first-line treatment for T1-3 SCC of the EAC. However, the efficacy of chemoradiotherapy in T4 cases was inadequate. Further research is warranted to elucidate the efficacy of treatments with more intensive antitumor effects for locally advanced EAC cancers.
Termites grown on seaweed media as dietary source of protein in the diets of African catfish (Clarias gariepinus)
Authors: Inyang-Etoh Aniema Paul , Eteng Sunday Urom , Ifon Honor Tajoes 
Published: June 2022

Abstract
A twelve-week feeding trial was conducted to examine the effect of termites grown on seaweed as a dietary protein source in the diets of African catfish (Clarias gariepinus). A total of 600 live African catfish specimens were used in the investigation, which were divided into four groups (ATD0, ATD1, ATD2, and ATD3) depending on their diets. The control diet (ATD0) was made with 60 percent commercial fishmeal (FM) and 40% soybean meal (SBM); ATD1, ATD2, and ATD3 were made with 60% crude protein obtained from termites (Macrotermes nigeriensis) grown on media containing only terrestrial grass (Cenchrus purpureus), only seaweed (Sagassum natans), and their combination (50:50), respectively. Results show that daily weight gain was higher in fish fed termite meal (ATM) based diets, while feed conversion ratio and viscerosomatic index were higher in control group. Inclusion of ATM in diets of African catfish did not affect nutrient digestibility, enzyme activities, and total bile acid levels in the different portions of the digestive tract. Essential amino acids and mineral composition of whole fish also showed insignificant differences between experimental groups. However, fatty acid composition in whole fish fed diet without termite meal was significantly lower than those fed diets containing termites grown on seaweed. The study concludes that raising catfish on feed made from termites grown on seaweed can provide a good source of essential fatty acids with high bioavailability potential in African catfish.
Morphological Analysis of Anterior Permanent Dentition in Chinese population via Cone-beam Computed Tomography
Authors: CHEN Yu , Lu Bingtai , Dai Yu , Yu Zujian , You Yuehua , Wu Bin , Yan Zhengbin 
Published: June 2022

Abstract
Background
Investigation of roots and canals is crucial in clinical assessment and treatment. Morphologies such as root length, cemento-enamel junction curvature (CEJ-C), root furcation and canal variations remain to be clarified.
Methods
In this retrospective study, we look into 4309 anterior teeth from 441 patients via cone-beam computed tomography (CBCT) in Chinese population, attempting to summarize the morphological characteristics of maxillary and mandibular anterior teeth.
Results
Maxillary (95% CI(mm): 13(16.57, 16.97), 23(16.31, 16.76)) and mandibular (95% CI(mm): 33(15.38, 15.72), 43(15.23, 15.57)) canines are observed with elevated root length compared with corresponding incisors (p < .0001, One-way ANOVA), while those of mandibular central (95% CI(mm): 31(12.07, 12.31), 41(12.10, 12.35)) and lateral (95% CI(mm): 32(13.23, 13.48), 42(13.27, 13.54)) incisor varied with significant difference (p < .0001, One-way ANOVA). The CEJ-C reveals significant increasement around incisors (95% CI(mm): 12(2.44, 2.60), 11(2.48, 2.63), 21(2.40, 2.55), 22(2.23, 2.39)) than canines (95% CI(mm): 13(1.95, 2.10), 23(1.83, 1.97)) in maxilla (p < .0001, Kruskal-Wallis). Root furcation is detected in mandibular canines and lateral incisors. Moreover, all types of Vertucci’s classification are noticed with two other new types.
Conclusions
The current findings help to optimize the root morphological understanding of Chinese population especially in endodontics. Moreover, CEJ-C facilitates clinical aesthetic interpretation and treatment in implantology and prosthodontic. However, further study should be carried out to investigate morphologies in-depth.
Nuclear orphan receptor ERRα promotes cisplatin resistance in breast cancer cells by targeting CCNE2
Authors: Liu Jiahui , Feng Jinwen , Xuan Junfeng , Huang Ling , Xiao Bin , Zhu Yi , Kuang Zhenzhan , Zhang Weiyun , Song Lijie , Zhou Ye , Wang Jie , Yang Chuanhong , Ding Chen , Zhang Hongbin , Li Linhai , Sun Zhaohui , Zhou Quan 
Published: June 2022

Abstract
Background
Chemotherapy is one of the most efficient therapeutic methods for cancer, but increasingly serious drug resistance is the main cause of cancer recurrence and treatment failure. Our study aimed to investigate the mechanisms by which upregulation of estrogen-related receptor α (ERRα) induced chemoresistance in breast cancer cells.
Methods
Immunohistochemistry (IHC) was used to determine the expression of ERRα in breast cancer and adjacent tissues. Functional analyses (in vitro and in vivo) were performed to confirm the role of ERRα in cancerogenesis and cisplatin chemoresistance in breast cancer. RNA-sequencing, ChIP-seq and dual luciferase assays were performed to identify the mechanisms by which ERRα promotes chemoresistance in breast cancer.
Results
ERRα was highly expressed in breast cancer tissues and the level of ERRα expression was determined to be significantly correlated with the expression level of human epidermal growth factor receptor 2 (HER2). Overexpression of ERRα promoted the proliferation and metastasis of breast cancer in vitro and in vivo while knockdown of ERRα suppressed. Moreover, ERRα was upregulated in chemoresistant breast cancer cells, while ERRα knockdown sensitized cisplatin treatment. Our results further demonstrated that ERRα enhanced the pluripotency of cancer cells and facilitated chemoresistance via upregulating CCNE2 by binding its promoter region. Overexpression of CCNE2 could reverse the sensitivity of breast cancer cells to cisplatin caused by ERRα depletion, thus resulting in accelerated tumor growth.
Conclusion
We identify that ERRα sustains the chemoresistance of breast cancer cells via regulating the transcription of CCNE2 and may serve as a therapeutic target in breast cancer.
Efficacy and Safety of Guselkumab and Adalimumab for Pustulotic Arthro-Osteitis and their Impact on Peripheral Blood Immunophenotypes
Authors: Ueno Masanobu , Miyagawa Ippei , Miyazaki Yusuke , Hanami Kentaro , Fukuyo Syunsuke , Kubo Satoshi , Nakayamada Shingo , Tanaka Yoshiya 
Published: June 2022

Abstract
Objectives: We compared the treatment effectiveness between guselkumab (GUS) and adalimumab (ADA) in patients with pustulotic arthro-osteitis (PAO). In addition, we performed peripheral blood immunophenotyping to elucidate the immunological background and analyzed the impact of therapeutic drugs to verify the validity of immunological phenotypes as therapeutic targets.Methods: Patients were treated with GUS 100 mg (GUS group; n = 12) and ADA 40 mg (ADA group; n = 13). Arthritis disease activity, skin lesion activity, and patient-reported outcomes (PROs) were evaluated and compared between the two groups. The retention rate and adverse events were evaluated. Comprehensive phenotyping of peripheral immune cells was performed in both groups, and phenotypes were compared before and after treatment.Results: At 6 months, both groups showed significant improvement in arthritis disease activity and PROs. In the GUS group, skin symptoms significantly improved. The 6-month continuation rates were 91.7% (11/12) and 69.2% (9/13) in the GUS and ADA groups, respectively. Adverse events occurred in 2/12 and 5/13 patients in the GUS (16.7%) and ADA (38.5%) groups, respectively. Peripheral blood immunophenotyping showed that the proportion of activated T helper (Th) 1 cells was significantly lower in patients with PAO than in healthy controls, and that the proportion of activated Th17 cells was significantly higher in patients with PAO, which significantly decreased after treatment with GUS.Conclusion: Although GUS and ADA have comparable efficacy for PAO, their impact on immunophenotypes varies. Precision medicine based on the immunological background of diseases should be considered.
Nutritional Security Aspects and Hygiene Practices among Street Food Vendors during Communicable Diseases Infection (COVID- 19 Pandemic)
Authors: Verma Ruchi , Mishra Sunita 
Published: June 2022

Abstract
Background: Nutritional security aspects and practicing good hygiene practices is an important component for ensuring human well-being. COVID-19, a global pandemic has had a bad effect on health of people around the world. Street food that is unsafe and unhealthy as it is sold in open areas could have been one of the causes of the spread of microbes, diseases. The aim of this study is to examine the knowledge of food nutritional security and hygiene practices among food vendors, during the Communicable Diseases (COVID- 19 Pandemic) that could have ensured consumer's health.Methods: A systematic questionnaire was used to collect the data, and sample randomly selected from Lucknow. Results were presented in terms of chi-square (χ 2) test, percentage, and p-value. Results: The result obtained by the compilation of the questionnaire shows that majority of handlers were well-versed about food hygiene. However, vendor lacked knowledge about cooking practices and storage of cooked food. It has been observed that 44 percent of food handler’s clean dirty utensils with soap and water, only 25% of food operators wash their hands in clean water each time before handling food, while 6 percent of operators used gloves while handling food. The overall food handler's food handling practices scores were lower than their knowledge scores based on the questionnaire responses. Conclusion: Results of the questionnaire shows that vendor has limited knowledge regarding nutritional security of the food and do not follow adequate hygiene practices, therefore awareness program should be promoted to increase knowledge of nutritional security and hygiene practices among vendors. The handlers should be made aware regarding fact of significance of nutritional security and hygiene practices to contain spread of microbes and communicable diseases.
TaqI polymorphism T/t genotypes at the vitamin D receptor gene (VDR) are associated with increased serum vitamin D levels in mild and moderate psoriasis vulgaris: A pilot study
Authors: Elneam Ahmed Ibrahim Abd , Al-Dhubaibi Mohammed Saleh , Bahaj Saleh salem , Mohammed Ghada Farouk 
Published: June 2022

Abstract
Background: Several types of polymorphisms in vitamin D receptor (VDR) have been found in psoriasis.Aim: this study looked at the role of the TaqI polymorphism in the VDR gene as a factor in changing plasma 25-hydroxyvitamin D (25(OH)D) levels in psoriasis patients and to see if it had any relationship with disease severity. Subjects and Methods: Clinical examination, serum (25(OH)D) levels measurement, molecular studies, TaqI genotyping by PCR and RFLP subjected for the two groups.Results: T/t genotypes of TaqI polymorphism genotypes were most common in patients, while t/t genotypes were more abundant in healthy subjects. The T allele was high in the patient group in comparing to the normal subjects, but there were no significant differences (P=0.421). Patients with T/t TaqI genotypes had higher levels of 25(OH)D) than T/T and t/t (P=0.004). Moderate psoriatic patients with the T/t genotype have relatively high 25(OH)D) levels than moderate patients with the T/t and t/t genotypes (P=0.001). Conclusion: The increase in 25(OH)D) titers in moderate patients is greater than that in mild and severe patients. T/t genotypes are associated with increased 25(OH)D) levels in moderate and mild patients.
Nocardia infection following ocular surface surgery
Authors: Wang Jingting , Lu Xiuhai , Wang Jungang , Wang Shuting , Shi Weiyun , Li Suxia 
Published: June 2022

Abstract
Objective: To investigate the clinical characteristics and treatment outcomes of Nocardia infection after ocular surface surgery. Methods: This is a retrospective study. Eight cases of culture-proven Nocardia infection, which developed within one month after ocular surface surgery were included. Demographics and clinical history of patients were investigated. Results: There were 8 eyes (2 left and 6 right) of 8 patients (5 males and 3 females), aged 27-65, with a median age of 52.9 years. Three cases underwent pterygium excision, three were subjected to conjunctival flap covering, and two were treated with lamellar corneal transplantation. The time interval between previous surgery and the onset of symptoms varied from 7 to 28 days (mean = 20.5 ± 7.13 days). All the cases presented grey-white infiltrates at the surgical incision site while appearing with six corneal ulcers and two conjunctival ulcers. Filaments of Nocardia were founded by confocal microscopy in two of the five cases. All responded poorly to medical therapy. Seven of the eight cases were treated with reoperation. Nocardia infection recurred in three cases after reoperation, and one was eviscerated.Conclusions: Surgical trauma is a risk factor for ocular Nocardia infection. Nocardia infection should be suspected when secondary infection occurs in a surgical incision with an atypical clinical presentation. Complete removal of lesions may lower the recurrence of Nocardia infection with poor drug treatment effects.
Dissecting the polygenic basis of atherosclerosis using disease associated cell state signatures
Authors: Örd Tiit , Lönnberg Tapio , Ravindran Aarthi , Niskanen Henri , Kiema Miika , Õunap Kadri , Maria Maleeha , Moreau Pierre , Mishra Pashupati , Palani Senthil , Virta Jenni , Liljenbäck Heidi , Aavik Einari , Roivainen Anne , Ylä-Herttuala Seppo , Laakkonen Johanna , Lehtimäki Terho , Kaikkonen Minna 
Published: June 2022

Abstract
Coronary artery disease (CAD) is a pandemic disease where up to half of the risk is explained by genetic factors. Advanced insights into genetic basis of CAD require deeper understanding of the contributions of different cell types, molecular pathways and genes to disease heritability. Here, we investigate the biological diversity of atherosclerosis associated cell states and interrogate their contribution to the genetic risk of CAD using single cell and bulk RNA sequencing (RNA-Seq) of mouse and human lesions. We identified thirteen disease-associated cell states which we characterized further by gene set functional profiling, ligand-receptor prediction and transcription factor inference. Importantly, Vcam1+ smooth muscle cell state genes contributed most to SNP-based heritability of CAD. In line with this, genetic variants near smooth muscle cell state genes and regulatory elements explained the largest fraction of CAD risk variance between individuals. Using this information for variant prioritization, we derived a hybrid polygenic risk score (PRS) that demonstrated improved performance over a classical PRS. Our results provide insights into the biological mechanisms associated with CAD risk, which could make a promising contribution to precision medicine and tailored therapeutic interventions in the future.
Experimental study of a pilot unit of a ground regenerator for greenhouses
Authors: Mukminov Ihor , Volgusheva Natalya , Georgiesh Catherina , Boshkova Irina 
Published: June 2022

The object of research: the process of heat exchange in a packing in the form of a dense layer of crushed stone as part of a thermal regenerator designed to utilize the low-grade heat of the air in a greenhouse.
Investigated problem: increasing the efficiency of air heat accumulation in the greenhouse during the daytime by a regenerative heat exchanger with a granular packing. Conducting experimental tests of regenerator models with a packing in the form of a dense layer of granular material under natural conditions is necessary to improve the design and optimize technological parameters.
The main scientific results: the analysis of temperature curves of air changes at the inlet and outlet of the regenerator installed in the mock-up greenhouse with a volume of 0.25 m3, and the packing material was carried out. During the heating period of the nozzle, the accumulated heat was Q=8•104 J, which can be used when the temperature in the greenhouse decreases. During the pause, the loss from the heat exchange channel to the environment is 48,000 J. The duration of the period was 358 min, while the average heat flux corresponded to 2.2 W. Heat losses during the pause period are significant due to its duration, however, the average heat transfer coefficient has a low value of k=2.3 W/m2K, so strengthening the insulation of the heat exchange channel is irrational.
The area of practical use of the results of the study: greenhouses, allowing the possibility of installing additional heat exchange equipment.
Innovative technological product: the innovativeness of the technical solution is determined by the possibility of using the heat of the air in the greenhouse and the implementation of contact heat exchange between the air flow and the packing particles. The design of the regenerator, the simplicity of its manufacture and operation, as well as the efficiency of air heat accumulation in the greenhouse during the daytime, allow to recommend it for use in greenhouse farms.
The scope of the innovative technological product: regenerative heat exchangers with a dense layer of granular material for the utilization of low-grade air flows generated in food production enterprises, ventilation systems and greenhouses
CRISPR: A Promising Tool for Cancer Therapy
Authors: Mohammad-Rafiei Fatemeh , Safdarian Esmat , Adel Bashir , Vandchali Noushin Rezaei , Navashenaq Jamshid Gholizadeh , Gheibihayat Seyed Mohammad 
Published: June 2022

Abstract:
The clustered regularly interspaced short palindromic repeats system, called
CRISPR, as one of the major technological advances, allows geneticists and
researchers to perform genome editing. This remarkable technology is quickly eclipsing
zinc-finger nucleases (ZFNs) and other editing tools, and its ease of use and accuracy
have thus far revolutionized genome editing, from fundamental science projects to
medical research and treatment options. This system consists of two key components: a
CRISPR-associated (Cas) nuclease, which binds and cuts deoxyribonucleic acid (DNA)
and a guide ribonucleic acid (gRNA) sequence, directing the Cas nuclease to its target
site. In the research arena, CRISPR has been up to now exploited in various ways
alongside gene editing, such as epigenome modifications, genome-wide screening,
targeted cancer therapies, and so on. This article reviews the current perceptions of the
CRISPR/Cas systems with special attention to studies reflecting on the relationship
between the CRISPR/Cas systems and their role in cancer therapy.
Association Between the Levels of Serum Free Testosterone and Lifestylerelated
Diseases in People Living with HIV
Authors: Yoshino Yusuke , Wakabayashi Yoshitaka , Kitazawa Takatoshi 
Published: June 2022

Background:
Recently, male hypogonadism was reported to be prevalent in people living
with HIV (PLWH), even in cases diagnosed based on the serum free testosterone level (fTST).
However, studies on the management of PLWH showing the relationship between male hypogonadism
and lifestyle-associated diseases, are sparse.

Objective:
This study evaluated the relationship between serum fTST levels and lifestyle-related
diseases in virologically stable PLWH.

Methods:
This study was a retrospective cohort single-center study. The study included HIVinfected
men on antiretroviral therapy, with available data on serum fTST levels at Teikyo University
Hospital between June 2020 and September 2020. Clinical
information was collected at the time
of fTST measurement. A simple regression analysis was used to identify continuous variables significantly
associated with serum fTST levels. Student’s t-test and Mann-Whitney U test were also
used to identify non-continuous variables that were significantly correlated with serum fTST levels.

Results:
Sixty male patients were evaluated. The median age was 47 (40-62) years. Low serum
fTST levels were significantly associated with old age, low hemoglobin and total cholesterol levels,
and high hemoglobin A1c levels. Non-use of INSTI and comorbid hypertension were also significantly
associated with low serum fTST levels.

Conclusions:
Hypertension and the serum hemoglobin A1c level as a standard parameter for diabetes
was significantly associated with low serum fTST levels in Japanese male PLWH. This study
suggested that sex-hormone replacement therapy could be a preferred option for PLWH with low
serum fTST levels to manage their long-term complications.