bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crosstalk between miRNA and protein expression profiles in nitrate exposed brain cells
Authors: Mishra Saumya , Sarkar Sana , Pandey Anuj , Yadav Sanjeev Kumar , Negi Renu , Yadav Sanjay , Pant A B 
Published: December 2022

Abstract
Growing evidence reported a strong association between the ingestion of nitrate and adverse health consequences in humans, including its detrimental impact on the developing brain. The present study identified miRNAs and proteins in SH-SY5Y human neuroblastoma cells and HMC3 human microglial cells using high throughput techniques in response to nitrate level most prevalent in the environment (mainly India) (X) and an exceptionally high nitrate level (5X) that can be reached in the near future. Cells were exposed to mixtures of nitrates for 72 h at doses of X and 5X, 320 mg/L and 1600 mg/L, respectively. OpenArray and LCMS analysis revealed that maximum deregulation in miRNAs and proteins was found in cells exposed to 5X dose. Top deregulated miRNAs include miR-34b, miR-34c, miR-155, miR-143, and miR-145. The proteomic profiles of both cell types include proteins that are potential targets of deregulated miRNAs. These miRNAs and their targeted proteins are involved in multiple functions, including cellular senescence, cell cycle, apoptosis, neuronal disorders, brain development, and homeostasis. Further, measuring mitochondrial bioenergetics in cells exposed to nitrate using a Seahorse XFp flux analyzer revealed that a 5X dose causes a significant reduction in oxygen consumption rate (OCR) and other bioenergetics parameters in both cell types. In summary, our studies have demonstrated that 5X dose of nitrate significantly alters cellular physiology and functions by deregulating several miRNAs and proteins. However, X dose of nitrate that is most prevalent in the environment has not caused any adverse effects on any cell type.
The Effect of escape room clinical evaluation method on satisfaction, learning, and preparedness to practice as interns of nursing students: a quasi-experimental quantitative study
Authors: ghiamikeshtgar najmeh , ghaljaei fereshteh , ghaljeh mahnaz , Mofrad zahra Pishkar , Taherizade Bentolhoda , Googhary Narjeskhatoun Sadeghi , mahmoodi nasrin , sharifi simin 
Published: December 2022

Abstract
Background
Clinical evaluation utilizing the escape room game is recognized as a novel method for assessing the team-oriented performance of learners. It is a tool for evaluating teamwork skills in clinical settings, which can boost student motivation and learning. This study aimed to investigate the effect of clinical evaluation through escape rooms and feedback provision through the PEARLS approach on pre-internship nursing students’ satisfaction, learning, and preparedness to practice as interns in the Faculty of Nursing and Midwifery, Zahedan University of Medical Sciences.
Method
The current research is a quasi-experimental quantitative study conducted with a census sample of 42 sixth-semester undergraduate nursing students at Zahedan University of Medical Sciences in 2022. The escape room method was utilized to evaluate entry preparedness into the clinical field. Reliable and valid researcher-made questionnaires were administered to assess the impact of the intervention on learning, satisfaction, and preparedness. The data were analyzed in SPSS version 26 using descriptive and inferential tests. The significance level was considered to be less than 0.05.
Results
26 males and 16 females constituted the 42 participants (mean age: 23.46 years). The clinical evaluation method of the escape room game was deemed satisfactory or highly satisfactory by 80% of students. From their perspective, escape rooms were definitely or highly effective in shaping their preparedness to enter the clinical field. Comparing the students’ mean learning scores (self-assessment of clinical skills) before and after the test revealed that their post-test scores were significantly higher than their pre-test scores (p < 0.001).
Conclusions
Considering the satisfaction of nursing students with the clinical evaluation using the escape room game and its impact on student learning, it is recommended that this method be implemented in all medical education departments.
Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemic
Authors: Krause Eva , Michel Janine , Puyskens Andreas , Hofmann Natalie , Rinner Thomas , Biere Barbara , Dorner Brigitte G. , Skiba Martin , Schaade Lars , Nitsche Andreas 
Published: December 2022

Abstract
Background
Over the course of the COVID-19 pandemic, laboratories worldwide have been facing an unprecedented increase in demand for PCR testing because of the high importance of diagnostics for prevention and control of virus spread. Moreover, testing demand has been varying considerably over time, depending on the epidemiological situation, rendering efficient resource allocation difficult. Here, we present a scalable workflow which we implemented in our laboratory to increase PCR testing capacity while maintaining high flexibility regarding the number of samples to be processed.
Methods
We compared the performance of five automated extraction instruments, using dilutions of SARS-CoV-2 cell culture supernatant as well as clinical samples. To increase PCR throughput, we combined the two duplex PCR reactions of our previously published SARS-CoV-2 PCR assay into one quadruplex reaction and compared their limit of detection as well as their performance on the detection of low viral loads in clinical samples. Furthermore, we developed a sample pooling protocol with either two or four samples per pool, combined with a specifically adapted SARS-CoV-2 quadruplex PCR assay, and compared the diagnostic sensitivity of pooled testing and individual testing.
Results
All tested automated extraction instruments yielded comparable results regarding the subsequent sensitivity of SARS-CoV-2 detection by PCR. While the limit of detection of the quadruplex SARS-CoV-2 PCR assay (E-Gene assay: 28.7 genome equivalents (ge)/reaction, orf1ab assay: 32.0 ge/reaction) was slightly higher than that of our previously published duplex PCR assays (E-Gene assay: 9.8 ge/reaction, orf1ab assay: 6.6 ge/reaction), the rate of correctly identified positive patient samples was comparable for both assays. Sample pooling with optimized downstream quadruplex PCR showed no loss in diagnostic sensitivity compared to individual testing.
Conclusion
Specific adaptation of PCR assays can help overcome the potential loss of sensitivity due to higher levels of PCR multiplexing or sample dilution in pooled testing. Combining these adapted PCR assays with different sample processing strategies provides a simple and highly adjustable workflow for resource-efficient SARS-CoV-2 diagnostics. The presented principles can easily be adopted in a variety of laboratory settings as well as be adapted to pathogens other than SARS-CoV-2, making it feasible for any laboratory that conducts PCR diagnostics.
Factors associated with public awareness of the relationship between alcohol use and breast cancer risk
Authors: Doyle Anne , O’Dwyer Claire , Mongan Deirdre , Millar Seán R. , Galvin Brian 
Published: December 2022

Abstract
Background
Public awareness of the carcinogenic effects of alcohol is low, particularly the association between alcohol use the risk of developing breast cancer. Breast cancer is the third most common cancer in Ireland and alcohol use remains high. This study examined factors related to awareness of the association between alcohol use and breast cancer risk.
Methods
Using data from Wave 2 of the national Healthy Ireland Survey, a representative sample of 7,498 Irish adults aged 15 + years, descriptive and logistic regression analyses were conducted to investigate relationships between demographic characteristics, type of drinker and awareness of breast cancer risk.
Results
A low level of awareness of the risk of alcohol use (drinking more than the recommended low–risk limit) associated with breast cancer was found, with just 21% of respondents correctly identifying the relationship. Multivariable regression analyses found that factors most strongly associated with awareness were sex (female), middle age (45—54 years) and higher educational levels.
Conclusion
As breast cancer is a prevalent disease among women in Ireland, it is essential that the public, in particular women who drink, are made aware of this association. Public health messages that highlight the health risks associated with alcohol use, and which target individuals with lower educational levels, are warranted.
The role of ambidextrous leadership and employee ambidexterity in enhancing service quality of care and creativity – A study of health professionals
Authors: Slåtten Terje , Mutonyi Barbara Rebecca , Nordli Anne Jørgensen , Lien Gudbrand 
Published: December 2022

Abstract
Background
It is important to understand how and in what ways a leadership style is capable to promote health professionals job performance. In this study we explore a relatively new leadership style, termed ambidextrous leadership. Ambidextrous leadership encompasses a leader capability to stimulate both exploitative and explorative activities in employees. Specifically, ambidextrous leadership is linked to two types of job-directed performance of health professionals’, respectively service quality of care and creativity. The study also examine what role employee ambidexterity seems to have in this relationship.
Methods
To test the conceptual model of this study, a cross-sectional survey was developed and data was gathered through convenience sampling of a total of N = 258 health professionals in home care services across nine various municipalities based in Norway. The study tested and analyzed the proposed hypotheses of this study through structural equation modeling partial least square, with SmartPLS 3 software. In addition to testing the direct relationships, the study also examined the indirect relationship through mediation testing by bootstrap.
Results
Ambidextrous leadership were found to have a direct impact on both employee’s service quality (\(\beta\) = 0.236) of care and employee ambidexterity (\(\beta\) = 0.395). The direct relationship between ambidextrous leadership and employee creativity were insignificant. However, the relationship ambidextrous leadership and service quality of care and creativity were both mediated of employee ambidexterity. Finally, the results reveal that employee creativity mediated the relationship between employee ambidexterity and service quality of care.
Conclusions
Although there are some differences, both ambidextrous leadership and employee ambidexterity are capable to promote job-directed performance of health professionals. Thus, a practical implication on the basis of the findings is that healthcare organizations should recruit, train and develop their leaders to become ambidextrous leaders. Doing so will have a direct impact on level of service quality as well as employee ambidexterity. In addition, acquiring ambidextrous leadership practices will also positively transform employee ambidexterity in a positive direction and indirectly stimulate employee’s creativity as well as strengthening health professionals service quality of care offering. Consequently, health organizations should be conscious of the multiple direct- and indirect effects of practicing ambidextrous leadership.
An improved VSG control strategy based on transient electromagnetic power compensation
Authors: Gao Changwei , Wang Wei , Huang Chongyang , Zheng Weiqiang 
Published: December 2022

Abstract
Virtual synchronous generator (VSG) not only increases the inertia of grid-connected system, but also brings the problem of active power oscillation under grid disturbance. Therefore, VSG control strategy and system model order reduction method with transient electromagnetic power compensation are proposed. The closed-loop active power small signal model of the system is established, and the influence of transient electromagnetic power compensation on the power stability of VSG is analyzed based on root locus method. By removing the items which have little influence on the stability of the system in the small signal model, the order is reduced to obtain the equivalent second-order model of the system. According to the second-order model, the quantitative design criteria of the system parameters are given. The proposed transient electromagnetic power compensation strategy not only increases the transient equivalent damping of the system, but also does not affect the primary frequency modulation characteristics and will not cause large overshoot of the output active power. The experimental results are consistent with the theoretical analysis, which testify the effectiveness and correctness of the system control strategy and the model reduction method.
Diagnosis of Neonatal Neurofibromatosis Type 1: a case report and review of the literature
Authors: Zheng Qiuying , Wang Ruijie , Xie Fusui , Zhao Xiaoli , Pei Nihui , Tao Hongwei , Ding Tingting , Liu Lei 
Published: December 2022

Abstract
Background: Neurofibromatosis Type 1 (NF1) is a rare genetic disorder characterized with the development of multiple benign tumors on the nerves and skin.
Case presentation: This report described a case of a neonate with a large mass observed on the left side of the maxillofacial region and cervical region at birth. Meantime, multiple cafe-au-lait macules (CALMs) were also seen on the trunk and both lower extremities.
Conclusions: In this case, ultrasonography was used as a diagnostic method to discuss the ultrasonographic and clinical features of the tumor.
Spatial representations of objects used away and toward the body: the effect of near and far space
Authors: Ruggiero Gennaro , Ruotolo Francesco , Nunziata Scila , Abagnale Simona , Iachini Tina , Bartolo Angela 
Published: December 2022

Abstract
An action with an object can be accomplished only if we encode the position of the object with respect to our body (i.e. egocentrically) and/or to another element in the environment (i.e. allocentrically). However, some actions with the objects are directed towards our body, such as brushing our teeth, and others away from the body, such as writing. Objects can be near the body, that is within arm reaching, or far from the body, that is outside arm reaching. The aim of this study was to verify if the direction of use of the objects influences the way we represent their position in both near and far space. Objects typically used towards (TB) or away from the body (AB) were presented in near or far space and participants had to judge whether an object was closer to them (i.e. egocentric judgment) or closer to another object (i.e. allocentric judgment). Results showed that egocentric judgments on TB objects were more accurate in near than in far space. Moreover, allocentric judgments on AB objects were less accurate than egocentric judgments in near but not in far space. These results are discussed with respect to the different role that visuomotor and visuospatial mechanisms play in near and in far space, respectively.
FLOURY ENDOSPERM 6 mutations enhance the sugary phenotype caused by the loss of ISOAMYLASE1 in barley
Authors: Matsushima Ryo , Hisano Hiroshi , Galis Ivan , Miura Satoko , Crofts Naoko , Takenaka Yuto , Oitome Naoko F , Ishimizu Takeshi , Fujita Naoko , Sato Kazushiro 
Published: December 2022

Abstract
Starch is a biologically and commercially important glucose polymer synthesized by plants as semicrystalline starch granules (SGs). Because SG morphology affects starch properties, mutants with altered SG morphology may be useful in breeding crops with desirable starch properties, including potentially novel properties. In this study, we employed a simple screen for mutants with altered SG morphology in barley (Hordeum vulgare). We isolated mutants that formed compound SGs together with the normal simple SGs in the endosperm and found that they were allelic mutants of the starch biosynthesis genes ISOAMYLASE1 (HvISA1) and FLOURY ENDOSPERM 6 (HvFLO6), encoding starch debranching enzyme and CARBOHYDRATE-BINDING MODULE 48-containing protein, respectively. We generated the hvflo6 hvisa1 double mutant and showed that it had significantly reduced starch biosynthesis and developed shrunken grains. In contrast to starch, soluble α-glucan, phytoglycogen, and sugars accumulated to higher levels in the double mutant than in the single mutants. In addition, the double mutants showed defects in SG morphology in the endosperm and in the pollen. This novel genetic interaction suggests that hvflo6 acts as an enhancer of the sugary phenotype caused by hvisa1 mutation.
Temporal associations of the COVID-19 related border restrictions and respiratory viral infections in New Zealand
Authors: Huang Q. Sue , Turner Nikki , Wood Timothy , Geoghegan Jemma , Anglemyer Andrew , McIntyre Peter , de Ligt Joep , Aminisani Nayyereh , Jelley Lauren , Jefferies Sarah , Dowell Tony , Trenholme Adrian , Brynes Cass , Balm Michelle , McIntosh Christine , Grant Cameron , Nesdale Annette , Dobinson Hazel , Campbell-Stokes Priscilla , Daniells Karen , Seeds Ruth , Jennings Tineke , Rensburg Megan , Cueto Jort , Caballero Ernest , John Joshma , Penghulan Emmanuel , Tan Chor , Ren Xiaoyun , Berquist Klarysse , O'Neill Meaghan , Marull Maritza , Yu Chang , McNeill Andrea , Kiedrzynski Tomasz , Roberts Sally , McArthur Colin , Stanley Alicia , Taylor Susan , Wong Conroy , Lawrence Shirley , Baker Michael , Kvalsvig Amanda , Werff Koen , McAuliffe Gary , Antoszewska Hannah , Dilcher Meik , Fahey Jennifer , Werno Anja , Elvy Juliet , Grant Jennifer , Addidle Michael , Zacchi Nicolas , Mansell Chris , Widdowson Marc-Alain , Thomas Paul , Consortium NPIsImpactOnFlu , Webby Richard 
Published: December 2022

Abstract
New Zealand (NZ)’s elimination of community transmission of influenza and respiratory syncytial virus (RSV) infections in May 2020, due to stringent COVID-19 countermeasures, provided a rare opportunity to assess the impact of border restrictions and relaxations on common respiratory viral infections over the subsequent two-years. Using multiple surveillance systems, we observed that border closure to most non-residents, and mandatory government-managed isolation and quarantine on arrival for those allowed to enter, appeared to be effective in keeping influenza and RSV infections out of the NZ community. Partial border relaxations through quarantine free travel with Australia and other countries were associated, within weeks, with importation of RSV and influenza into NZ in 2021 and 2022. Border restrictions did not have effect on community transmission of other respiratory viruses such as rhinovirus and parainfluenza virus type 1. These data can inform future pandemic influenza preparedness as well as provide insights into effective strategies to plan and model the impact of seasonal influenza, RSV, and other respiratory viral infections.
Prevalence and clinical outcome of Omicron breakthrough infection in patients with hematologic disease: a prospective observational cohort study
Authors: Narita Kentaro , Ikeda Daisuke , Seki Mizuki , Fukumoto Ami , Tabata Rikako , Uesugi Yuka , Miura Daisuke , Takeuchi Masami , Doi Masahiro , Umezawa Yuka , Otsuka Yoshihito , Matsue Kosei 
Published: December 2022

Abstract
To explore the prevalence and outcomes of the severe acute respiratory syndrome virus-2 Omicron infection in hematologic disease (HD), we performed a prospective observational study on 922 patients with HD and 88 healthcare workers (HCW). We identified 75 and 12 Omicron breakthrough infections in HD and HCW, respectively. In multivariate analysis, older age and use of anti-CD20 antibody within one year were significant independent predictors of breakthrough infection in HD who received two doses of vaccination, but these were not significant for three doses. Of the 75 HD patients infected with Omicron, 66 had mild symptoms and recovered completely, while nine patients required oxygen therapy, three of whom eventually died. In nine patients with longitudinal polymerase chain reaction data available, the five patients who were treated with anti-CD20 antibodies plus bendamustine had prolonged viral shedding. The absolute T-cell subset counts were significantly lower in patients with prolonged viral shedding than in those without. Overall, the symptoms of Omicron infection in HD patients other than those with bendamustine were generally mild and prompt viral clearance was achieved. Even in immunocompromised HD patients, other than those treated with bendamustine, Omicron infection may not require the rigorous infection control as with previous variants.
Antimicrobial resistance and evolution of genomic epidemiology of multidrug- resistant Salmonella enterica serovar Enteritidis ST11 in China
Authors: Liu Jie , Hou houyue , Zhao Linna , Chen Gang , Chen Jialiang , Zhao Qiang , Ye Liyan , Cui Shenghui , Wang Chengbin 
Published: December 2022

Abstract
In recent years, with the change of multidrug resistant strains, the genetic characteristics of foodborne Salmonella enterica serovar Enteritidis and clinical isolates have changed, and ST11 is the most common genotype associated with the S. Enteritidis isolates from humans and food animals across the world. In this study, the phenotypes and genetic characteristics of antibiotic resistance in foodborne and clinical S. Enteritidis isolates were analyzed. The results showed that the antibiotic resistance of foodborne S. Enteritidis isolates from 2017 was more severe than that of clinical isolates from 2013 to 2017, with a wider antibiotic resistance spectrum and higher antibiotic resistance rate. The genetic characteristics of S. Enteritidis from different sources were also different. Compared with clinical isolates, food-borne S. Enteritidis carried more antibiotic resistance genes, and the context characteristics of antibiotic resistance genes were different. For example, blaCTX−M and dfrA17 were only present on the plasmid of clinical S. Enteritidis but existed in the plasmid and chromosome of foodborne S. Enteritidis, and the carrying rate of floR was higher than that of clinical isolates. Analysis of the reasons suggests that the isolation of foodborne S. Enteritidis is late, the gene evolution is faster, and some resistance genes can be integrated and stably exist on the chromosome, which speeds up the evolution of resistance genes and provides more mechanisms for resistance to antibiotics. Compared the virulence genes of S. Enteritidis from different sources, we found that except for the distribution of virulence plasmids, there was no difference in the distribution of other virulence genes, so we believe that foodborne S. Enteritidis has the potential to cause clinical infection, and once the evolution of these drug resistance genes starts to expand, it will cause a problem for clinical treatment. Therefore, we should always monitor the changes in drug resistance patterns of S. Enteritidis from different sources.