bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do the means affect the ends? Radical tactics influence motivation and action tendencies via the perceived legitimacy and efficacy of those actions
Authors: Lizzio‐Wilson Morgana , Thomas Emma F. , Louis Winnifred R. , Amiot Catherine E. , Bury Simon M. , Molenberghs Pascal , Decety Jean , Crane Monique F. 
Published: February 2022

AbstractIdentity, injustice and group efficacy are key motivations for collective action engagement. However, little work has examined factors that influence their emergence. Across three studies (total N = 938), we test whether exposure to different actions (i.e., radical or conventional) and the perceived legitimacy and efficacy of those actions (‘the means’) predict observers’ sense of injustice, identity, group efficacy about the issue, and in turn, future action engagement (‘the ends’). As expected, radical (versus conventional) actions were perceived as less legitimate and effective. These evaluations indirectly predicted lower action via diminished identification and injustice, respectively. Paradoxically, legitimacy and efficacy evaluations also indirectly predicted higher radical and conventional action via diminished group efficacy. Thus, collective action is shaped by and reciprocally influences injustice, identity, and group efficacy. Simultaneous exposure to conventional and radical actions also offset these effects, indicating that conventional actions can mitigate the indirect effects of radical tactics.
Stress‐induced increase of monoclonal antibody production in CHO cells
Authors: Schellenberg Jana , Nagraik Tamanna , Wohlenberg Ole Jacob , Ruhl Sebastian , Bahnemann Janina , Scheper Thomas , Solle Dörte 
Published: February 2022

AbstractMonoclonal antibodies (mAbs) are of great interest to the biopharmaceutical industry due to their widely used application as human therapeutic and diagnostic agents. As such, mAb require to exhibit human‐like glycolization patterns. Therefore, recombinant Chinese hamster ovary (CHO) cells are the favored production organisms; many relevant biopharmaceuticals are already produced by this cell type. To optimize the mAb yield in CHO DG44 cells a corelation between stress‐induced cell size expansion and increased specific productivity was investigated. CO2 and macronutrient supply of the cells during a 12‐day fed‐batch cultivation process were tested as stress factors. Shake flasks (500 mL) and a small‐scale bioreactor system (15 mL) were used for the cultivation experiments and compared in terms of their effect on cell diameter, integral viable cell concentration (IVCC), and cell‐specific productivity. The achieved stress‐induced increase in cell‐specific productivity of up to 94.94.9%–134.4% correlates to a cell diameter shift of up to 7.34 μm. The highest final product titer of 4 g/L was reached by glucose oversupply during the batch phase of the process.
Wetlandscape (dis)connectivity and fragmentation in a large wetland (Haiderpur) in west Ganga plains, India
Authors: Singh Manudeo , Sinha Rajiv , Mishra Arjit , Babu Suresh 
Published: February 2022

AbstractWetlands are dynamic ecosystems that require continuous monitoring and assessment of degradation status to design strategies for their sustainable management. While hydrology provides the primary functional control for the wetland ecosystem, the loss of landscape connectivity influences wetland degradation in a major way as it leads to fragmentation. This article aims to integrate hydrogeomorphic and ecological concepts for the assessment of degradation status and its causal factors for a large wetland in the western Ganga plains, India, the Haiderpur, using a wetlandscape approach. We have used a remote‐sensing‐based approach, which offers a powerful tool for assessing and linking cross‐scale structures, functions, and controls in a wetlandscape. The Haiderpur, a Ramsar site since December 2021, is an artificial wetland located on the right bank of the Ganga River wherein the inflows are controlled by a barrage constructed on the Ganga River apart from smaller tributaries flowing in from the north. A novel aspect of this work is the integration of river dynamics and its connectivity to the wetlandscape to understand the spatiotemporal variability in the waterspread area in the wetland. In this work, we have developed an integrated wetlandscape assessment approach by evaluating wetland's geomorphic and hydrological connectivity status for the period 1993–2019 (25 years) across three different spatial scales – regional, catchment, and wetland. We have highlighted the ecological implications of connectivity and patch dynamics for developing sustainable wetland management plans.
Comparative Toxicity of Aquatic Per- and Polyfluoroalkyl Substance Exposure in Three Species of Amphibians
Authors: Flynn R. Wesley , Hoover Gary , Iacchetta Michael , Guffey Samuel , de Perre Chloe , Huerta Belinda , Li Weiming , Hoverman Jason T. , Lee Linda , Sepúlveda Maria S. 
Published: February 2022

Abstract
Per- and polyfluoroalkyl substances (PFAS) are contaminants of concern due to their widespread occurrence in the environment, persistence, and potential to elicit a range of negative health effects. Per- and polyfluoroalkyl substances are regularly detected in surface waters, but their effects on many aquatic organisms are still poorly understood. Species with thyroid-dependent development, like amphibians, can be especially susceptible to PFAS effects on thyroid hormone regulation. We examined sublethal effects of aquatic exposure to four commonly detected PFAS on larval northern leopard frogs (Rana [Lithobates] pipiens), American toads (Anaxyrus americanus), and eastern tiger salamanders (Ambystoma tigrinum). Animals were exposed for 30 days (frogs and salamanders) or until metamorphosis (toads) to 10, 100, or 1000 μg/L of perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorohexane sulfonate (PFHxS), or 6:2 fluorotelomer sulfonate (6:2 FTS). We determined that chronic exposure to common PFAS can negatively affect amphibian body condition and development at concentrations as low as 10 µg/L. These effects were highly species dependent, with species having prolonged larval development (frogs and salamanders) being more sensitive to PFAS than more rapidly developing species (toads). Our results demonstrate that some species could experience sublethal effects at sites with surface waters highly affected by PFAS. Our results also indicate that evaluating PFAS toxicity using a single species may not be sufficient for accurate amphibian risk assessment. Future studies are needed to determine whether these differences in susceptibility can be predicted from species' life histories and whether more commonly occurring environmental levels of PFAS could affect amphibians. Environ Toxicol Chem 2022;41:1407–1415. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Revival of the heterologous prime‐boost technique in COVID‐19: An outlook from the history of outbreaks
Authors: Siddiqui Amna , Adnan Alishba , Abbas Munib , Taseen Shafaq , Ochani Sidhant , Essar Mohammad Yasir 
Published: February 2022

AbstractBackgroundThe heterologous prime‐boost vaccination technique is not novel as it has a history of deployment in previous outbreaks.AimHence, this narrative review aims to provide critical insight for reviving the heterologous prime‐boost immunization strategy for SARS‐CoV‐2 vaccination relative to a brief positive outlook on the mix‐dose approach deployed in previous and existing outbreaks (ie, Ebola virus disease (EVD), malaria, tuberculosis, hepatitis B, HIV and influenza virus).Methodology and MaterialsA Boolean search was carried out to find the literature in MEDLINE‐PubMed, Clinicaltrials, and Cochrane Central Register of Controlled Trials databases up till December 22, 2021, using the specific keywords that include “SARS‐CoV2”, “COVID‐19”, “Ebola,” “Malaria,” “Tuberculosis,” “Human Immunodeficiency Virus,” “Hepatitis B,” “Influenza,” “Mix and match,” “Heterologous prime‐boost,” with interposition of “OR” and “AND.” Full text of all the related articles in English language with supplementary appendix was retrieved. In addition, the full text of relevant cross‐references was also retrieved.ResultsTherefore, as generally evident by the primary outcomes, that is, safety, reactogenicity, and immunogenicity reported and updated by preclinical and clinical studies for addressing previous and existing outbreaks so far, including COVID‐19, it is understood that heterologous prime‐boost immunization has been proven successful for eliciting a more efficacious immune response as of yet in comparison to the traditional homologous prime‐boost immunization regimen.DiscussionAccordingly, with increasing cases of COVID‐19, many countries such as Germany, Pakistan, Canada, Thailand, and the United Kingdom have started administering the heterologous vaccination as the technique aids to rationalize the usage of the available vaccines to aid in the global race to vaccinate majority to curb the spread of COVID‐19 efficiently. However, the article emphasizes the need for more large controlled trials considering demographic details of vaccine recipients, which would play an essential role in clearing the mistrust about safety concerns to pace up the acceptance of the technique across the globe.ConclusionConsequently, by combatting the back‐to‐back waves of COVID‐19 and other challenging variants of concerns, including Omicron, the discussed approach can also help in addressing the expected evolution of priority outbreaks as coined by WHO, that is, “Disease X” in 2018 with competency, which according to WHO can turn into the “next pandemic” or the “next public health emergency,” thus would eventually lead to eradicating the risk of “population crisis.”
Mammary‐type myofibroblastoma of the perineum: Typical or rare location?
Authors: Naito Akihiro , Takeshima Yuta , Takahashi Sayuri 
Published: February 2022

IntroductionMammary‐type myofibroblastoma is a rare benign tumor, mainly arising along the embryonal mammary ridge. We report a rare case of mammary‐type myofibroblastoma of the perineum.Case presentationA 37‐year‐old Japanese man presented with a 20 mm, progressively‐growing painless mass in the right perineum. Computed tomography showed a subcutaneous tumor with a strong contrast effect. Upon total resection, pathology showed a spindle‐cell tumor positive for desmin but negative for CD34. Further immunohistochemistry showed loss of Rb expression, leading to differential diagnosis. We could not evaluate the exact rarity of the perineal location due to categorization in past reports.ConclusionDue to the similarities between mammary and anogenital tissue, we suggest that tallying perineal and vulvar areas separately from the embryonic mammary ridge sites may be beneficial in gaining insight into the pathophysiology of this tumor.
Pseudoexfoliation syndrome: The critical role of the extracellular matrix in pathogenesis and treatment
Authors: Mastronikolis Stylianos , Pagkalou Marina , Baroutas Giorgos , Kyriakopoulou Konstantina , Makri Οlga E. , Georgakopoulos Constantinos D. 
Published: February 2022

AbstractPseudoexfoliation syndrome (PEXS) is an age‐related condition manifesting mainly in ocular tissues. PEXS is manifested through excess aggregation of fibrillary extracellular material at the anterior part of the eye that consists of a plethora of biomolecules, such as different proteoglycans (PGs) and glycosaminoglycans. PEXS is often linked to increased intraocular pressure, and can also lead to pseudoexfoliation glaucoma with very poor prognosis. Various stimuli are known to affect PEXS, including oxidation stress (OS), UV radiation and osmotic pressure. OS, is prominently involved on the progression of the syndrome as it promotes fibrogenesis, possibly via the induction of transforming growth factor‐β (TGF‐β) and other biomolecular effectors. In addition, PEXS initiation is tightly connected with the dysregulation of extracellular matrix (ECM) homeostasis since aberrant expression of ECM molecules is linked to both the accumulation and low degradation of pseudoexfoliation material. This article aims at uncovering the crucial role of various ECM effectors such as lysyl oxidase‐like proteins, matrix metalloproteinases, and TGF‐β1, as well as the biochemical pathways involved in the development and the progression of the PEXS.
Exploring resilience processes of Filipino migrant domestic workers: A multisystemic approach
Authors: Garabiles Melissa R. , Mayawati Elisabeth H. , Hall Brian J. 
Published: February 2022

AbstractThis study identified resilience processes at the individual, family, and community levels among Filipino migrant domestic workers (MDWs). Resilience processes highlight strengths and resources that can enhance positive adaptation to problems that affect this vulnerable migrant group. Data came from focus groups and key informant interviews involving 27 MDWs and 7 key informants. Data was analyzed using thematic analysis and organized according to the socioecological model. Results revealed 7 resilience processes. Financial coping, health management, and spirituality were found across levels. Companionship and emotional support are transacted within family and community levels, whereas expanding knowledge and support occurs at individual and community levels. MDWs and their families prioritize each family member, whereas the community provides legal support. Resilience processes exist across ecological levels. However, individual resilience processes are insufficient, thus necessitating collective agency through familial and community resilience processes and building social structures that facilitate resilience.
Hazard pay for internal medicine resident physicians during the COVID‐19 pandemic: A national survey of program directors
Authors: Uthlaut Brian , Catalanotti Jillian , Kisielewski Michael , McGarry Kelly , Finn Kathleen 
Published: February 2022

AbstractBackgroundHazard pay for resident physicians has been controversial in the COVID‐19 pandemic. Program director (PD) beliefs about hazard pay and the extent of provision to internal medicine (IM) residents are unknown.ObjectiveTo evaluate hazard pay provision to residents early in the COVID‐19 pandemic and pandemic and residency program characteristics associated with hazard pay.Design, Setting, and ParticipantsA nationally representative survey was conducted of 429 US/US territory‐based IM PDs from August to December 2020.Main Outcome and MeasuresHazard pay provision and PD beliefs about hazard pay were tested for association with factors related to the pandemic surge and program characteristics.ResultsResponse rate was 61.5% (264/429); 19.5% of PDs reported hazard pay provision. PD belief about hazard pay was equivocal: 33.2% agreed, 43.1% disagreed, and 23.7% were uncertain. Hazard pay occurred more commonly in the Middle‐Atlantic Census Division (including New York City) and with earlier surges and greater resident participation in COVID‐19 patient care. Hazard pay occurred more commonly where PDs supported hazard pay (74.5% vs. 22.1%, p = .018). Reasons most frequently given in support of hazard pay were essential worker status, equity, and schedule disruption. Those opposed cited professional obligation and equity.ConclusionHazard pay for IM residents early in the COVID‐19 pandemic was nominal but more commonly associated with heavily impacted institutions. Although PD beliefs were mixed, positive belief was associated with provision. The unique role of residents as both essential workers and trainees might explain our varied results. Further investigation may inform future policy, especially in times of crisis.
Long‐term functional outcomes and mortality after hospitalization for extracranial hemorrhage
Authors: Parks Anna L. , Jeon Sun Y. , Boscardin W. John , Steinman Michael A. , Smith Alexander K. , Covinsky Kenneth E. , Fang Margaret C. , Shah Sachin J. 
Published: February 2022

AbstractBackgroundThe effects of extracranial hemorrhage (ECH), or bleeding outside the brain, are often considered transient. Yet, there are few data on the long‐term and functional consequences of ECH.ObjectiveDefine the association of ECH hospitalization with functional independence and survival in a nationally representative cohort of older adults.DesignLongitudinal cohort study.Settings and ParticipantsData from the Health and Retirement Study from 1995 to 2016, a nationally representative, biennial survey of older adults. Adults aged 66 and above with Medicare linkage and at least 12 months of continuous Medicare Part A and B enrollment.InterventionHospitalization for ECH.Main Outcomes and MeasuresAdjusted odds ratios and predicted likelihood of independence in all activities of daily living (ADLs), independence in all instrumental activities of daily living (IADLs) and extended nursing home stay. Adjusted hazard ratio and predicted likelihood for survival.ResultsIn a cohort of 6719 subjects (mean age 77, 59% women) with average follow‐up time of 8.3 years (55,767 person‐years), 736 (11%) were hospitalized for ECH. ECH was associated with a 15% increase in ADL disability, 15% increase in IADL disability, 8% increase in nursing home stays, and 4% increase in mortality. After ECH, subjects became disabled and died at the same annual rate as pre‐ECH but never recovered to pre‐ECH levels of function. In conclusion, hospitalization for ECH was associated with significant and durable declines in independence and survival. Clinical and research efforts should incorporate the long‐term harms of ECH into decision‐making and strategies to mitigate these effects.
An ecosystem perspective on tourism: The implications for tourism organizations
Authors: Hillebrand Bas 
Published: February 2022

AbstractThis conceptual paper provides a theoretical perspective on tourism that goes beyond merely acknowledging the fact that tourism organizations have to take into account multiple actors. It contributes to the tourism literature by (1) conceptualizing ecosystems in such a way that it embraces the complexity inherent in tourism ecosystems and enabling a truly holistic perspective on tourism and (2) describing the organizational capabilities that are needed for dealing with this complexity. Four distinct but interrelated capabilities are distinguished: mapping the ecosystem, dealing with tension, involving the ecosystem, and effectuating the ecosystem.
LINC00958/miR‐627 signal axis regulates the proliferation, migration, and invasion of thyroid papillary carcinoma cells by TRIM44
Authors: Li Ya‐Qiong , Wang Ling‐Cheng , Li Ai‐Xia , Huang Wei , Song Ying , Wang Wei 
Published: February 2022

AbstractPapillary thyroid cancer (PTC) has attracted much attention due to its high morbidity and severe metastasis. Long noncoding RNA ENST00000504230 (LncRNA ENST00000504230, known as LINC00958) was overexpressed in many cancers and associated with cancer development. However, its underlying mechanism in PTC remains unclear. PTC tissues and corresponding adjacent tissues were collected for measuring the expression of LINC00958 and miR‐627. MiR‐627 and TRIM44 expressions were measured in in vitro cultured PTC cell lines (B‐cpap and IHH4 cells) transfected with sh‐LINC00958 or miR‐627 mimic using RT‐qPCR and western blot. Cell proliferation, migration, and invasion were measured by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide (MTT) and Transwell assays, respectively. Dual‐luciferase reporter assay was performed to evaluate the target association between miR‐627 and TRIM44. LINC00958 was up‐regulated in PTC tissues and cells, while the expression of miR‐627 was lowly expressed. Knockdown of LINC00958 inhibited the proliferation, migration, and invasion by elevating miR‐627 expression in PTC cells. TRIM44 was confirmed as a target of miR‐627. Overexpression of miR‐627 in PTC inhibited the proliferation, migration, and invasion by down‐regulating the expression of TRIM44. LINC00958 promoted proliferation, migration, and invasion in PTC by down‐regulating miR‐627 and activating TRIM44, indicating the potential therapeutic effect of LINC00958 on PTC.
LncRNA small nucleolar RNA host gene 16 reduces sepsis‐induced myocardial damage by regulating miR‐421/suppressor of cytokine signaling 5 axis
Authors: Yu Min , Xie Dan , Hu Cai‐Ying , Cui Yue 
Published: February 2022

AbstractCurrently, sepsis‐induced cardiomyopathy (SIC) remains as one of the most critical clinical syndromes in terminally ill patients. Noncoding RNAs (including microRNAs and long noncoding RNAs) are implicated in both the onset and development of SIC. We herein investigated the functional role and molecular target of long noncoding RNA small nucleolar RNA host gene 16 (SNHG16) in an in vitro SIC model of H9c2 myocardial cells. We used lipopolysaccharide (LPS) as endotoxin to treat H9c2 cells to mimic SIC damages. Cell Counting Kit 8 and apoptosis assay were performed to assess cell proliferation and cell death. Quantitative real‐time‐PCR and Western blot were employed to examine gene expression level at mRNA and protein level. Dual luciferase assay is used to validate the functional interactions between SNHG16/mi‐R421 and miR‐421/suppressor of cytokine signaling 5 (SOCS5). Inflammatory cytokines were measured by ELISA. Superoxide dismutase and malondialdehyde measurement was performed to assess oxidative stress, which was further confirmed by 2′,7′‐dichlorofluorescin diacetate staining. Our data demonstrated that in the LPS‐induced sepsis model of myocardial cells, SNHG16 overexpression downregulated the expression level of miR‐421, which sustained the expression of SOCS5 to alleviate the adverse effects of LPS, such as apoptosis, pro‐inflammatory cytokines, and oxidative stress. Our data suggest that SNHG16 functions as a ceRNA to maintain SOCS5 level by targeting miR‐421, thereby attenuating LPS‐induced myocardial cell damages. Targeting miR‐421 or modulating lncRNA SNHG16 level may be leveraged as a beneficial strategy against sepsis‐induced cellular damage in cardiomyocytes.
Effects of restoration strategies on wetland: A case‐study of Xinqiang River National Wetland Park
Authors: Lin Yang , Wang Deming , Farooq Taimoor Hassan , Luo Kai , Wang Weiyue , Qin Mingxun , Chen Shiqi 
Published: February 2022

AbstractAccurately understanding the characteristics of a wetland ecological restoration plant communities and their species diversity relationships is the key to improving wetland management and biodiversity protection. To see the effects of restoration strategies implemented in Xinqiang River National Wetland Park, China, we studied 4 models for ecological restoration, namely protection and conservation (PC), reasonable utilization (RU), restoration and reconstruction (RR) and the building of constructed wetland (CW). We then analyzed the features of the plant communities, species diversity and soil properties, and carried out a correlation analysis between plant communities and the species diversity. Finally, we selected four key communities and analyzed their features and soil properties using a symmetric matrix. Results showed that the soil in the PC model had the highest amount of available phosphorus, available nitrogen and pH value. The soil had higher moisture content in the RU model and CW model than the other 2 models. Based on the correlation analysis, we found that there are obvious common characteristics among different ecological restoration types, that is, the diversity characteristics of tree layer, shrub layer and herb layer have obvious differentiation characteristics. We identified four features of the community characteristics that could improve species diversity: SA, TH, HH and SCD. The results of this study have provided a theoretical basis that there is a coupling relationship between ecological community characteristics and species diversity. The above coupling results have practical significance for maintaining good ecological structure and diversity protection.