bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aspectos Epidemiológicos e Diagnóstico da Fibromialgia na Região Norte do Brasil
Authors: Alves Rafaela de Carvalho , Nepomuceno Victor Rodrigues , Marson Poliana Guerino , Bartholomeu Neto João , Silveira Janne Marques , Rodrigues Elizângela Sofia Ribeiro , Gomes Andressa de Oliveira , Porfírio Polyana Martins Neiva , Araujo Warly Neves de , Silva Karla Camila Correia da 
Published: March 2022

Introdução: A fibromialgia é considerada como uma síndrome reumatológica de diagnóstico clínico a partir do relato do paciente em relação à presença de algia difusa por mais de três meses, sem trauma, de caráter não inflamatório, associada a distúrbios cognitivos e do sono, além de fadiga e rigidez. Classificada como o segundo diagnóstico mais encontrado em doenças reumatológicas, impacta negativamente na capacidade funcional dos pacientes devido à complexidade da etiopatogenia e suas sequelas. Dados epidemiológicos desta enfermidade em pacientes do norte do Brasil são escassas, tornando tal pesquisa de relevância. Objetivo Geral: Traçar o perfil epidemiológico da fibromialgia em pacientes diagnosticadas do departamento de referência em reumatologia do município de Gurupi-TO. Materiais e Métodos: Foram selecionadas 60 mulheres com idade entre 18 e 75 anos, diagnosticadas com fibromialgia. Por meio de entrevista, as voluntárias responderam aos questionários de levantamento epidemiológico (com caracterização do perfil sociodemográfico e do histórico da doença). Resultados: A amostra de fibromiálgicas do Sul do Estado do Tocantins apresentou perfil epidemiológico similar ao restante do Brasil, com baixa escolaridade e elevados níveis de intensidade álgica.
Ações de saúde pública no Brasil contra o Diabetes Mellitus: uma revisão integrativa
Authors: Souza Lizandra Ellem Silva de , Souza Alessandro Ruan Silva de , Soares Gustavo Henrique dos Santos , Apolinário Joelma Maria dos Santos da Silva , Ferreira Paula Castro , Andrade Suzane Oliveira , Alves Nathálya Faria , Vieira Jade Oliveira , Silva João Felipe Tinto , Silva Fhillipe Ferreira Deodato da , Farias Anderson Fernandes de Carvalho , Henrique Giuliano Araújo 
Published: March 2022

Este estudo tem como objetivo descrever as principais ações de saúde pública com pacientes com Diabetes Mellitus através de uma análise da literatura. Trata-se de uma revisão da literatura com abordagem qualitativa realizada no periodo de dezembro de 2021. Foi realizada através de artigos disponíveis nas bases de dados Literatura Latino Americana e do Caribe em Ciências da Saúde (LILACS), Medical Literature Analysis and Retrieval System Online (MEDLINE) e Scientific Electronic Library Online (SCIELO) que abordassem como a saúde pública contribui para o controle da diabetes mellitus, para isso foi utilizado os seguintes descritores: Diabetes mellitus; Saúde Pública com uso do operador boleano AND. Totalizou um total de 1500 artigos, que após aplicação dos critérios de inclusão e exclusão, 12 artigos foram incluídos no presente estudo após leitura dos mesmos. No decorrer de toda a análise dos artigos foi possível observar que a atenção básica de saúde é primordial para o acompanhamento de pessoas com diabete mellitus e que intervenções vindas da atenção primária é indispensável para o controle da doença. Concluiu-se que o acompanhamento, orientações e disponibilidade de medicações contribui para adesão ao tratamento e controle dos riscos causados pela doença.
Correlação do Ângulo Goníaco e altura do Forame Mentoniano, avaliados por imagens de tomografia computadorizada da mandíbula: Odontologia Forense
Authors: Araújo Brenda Silva , Barros Luana de Souza , Martins Marcelo Tarcísio , Macedo Priscila Faquini , Chaves Hebertt Gonzaga dos Santos 
Published: March 2022

O conhecimento da anatomia é um dos fatores primordiais para o sucesso da comparação de registros dentários durante uma investigação de um indivíduo na odontologia forense. Para realizar esse tipo de procedimento, o profissional deve estar apto a identificar e diferenciar estruturas anatômicas do complexo maxilofacial. O objetivo desse estudo foi verificar se existe correlação entre ângulo goníaco e o forame mentoniano a idade e o gênero, de pacientes submetidos à exames de Tomografia Computadorizada da mandíbula. A metodologia desse trabalho baseou-se na obtenção e análise dos arquivos DICOM, de forma que estas imagens foram convertidas para um programa específico de tratamento de imagens ImplantViewer (Anne Solutions, São Paulo, Brasil), e avaliadas utilizando a Janela Panorâmica e Cortes Transversais de Tomografias Computadorizadas. Indivíduos do gênero masculino apresentaram o forame mentoniano mais distante da crista alveolar do que os indivíduos do gênero feminino. A TCFC é uma importante ferramenta na visualização das estruturas anatômicas, favorecendo as atividades inerentes a Odontologia Forense. Neste estudo, o gênero masculino apresentou o forame mentoniano mais distante da crista alveolar do que o gênero feminino.
Efektivitas Pengawetan Kayu Gmelina Menggunakan Akar Tuba Terhadap Serangan Rayap Tanah (The Effectiveness Of Preservation Of Gmelina Wood Using Tubal Roots Against Subterranean termites Attacks)
Authors: Hadjar Nurhayati , Pujirahayu Niken , Uslinawaty Zakiah , Hasrudin Hasrudin 
Published: March 2022

Abstract: The purpose of this study was to determine the effectiveness of the use of two types of tuba root extract, namely milk sap tuba root and clear sap tuba root in Wood Preservation of Gmelina (G. arborea Roxb) against subterranean termites. The method of testing against subterranean termites is using the burial method for 12 weeks. Wood resistance is calculated from the percentage of weight loss and the degree of termite attack on wood according to SNI 01-7207-2006 concerning the classification of wood resistance to subterranean termite attacks. the results of the study, it was concluded that the use of preservatives for milk sap tuba root and clear sap tuba root with concentrations of 2% and 4% were not considered effective for preserving Gmelina wood from subterranean termites. Keywords: Gmelina wood, preservation, milk sap tuba root, clear sap tuba root, subterranean termites, loss of weight, degree of attack.Abstrak: Tujuan dari penelitian ini adalah untuk mengetahui efektifitas penggunaan dua jenis ekstrak akar tuba yaitu akar tuba getah susu dan akar tuba getah bening dalam pengawetan kayu Gmelina (G. arborea Roxb) terhadap Rayap Tanah. Metode pengujian rayap tanah menggunakan metode penguburan selama 12 minggu. Ketahanan kayu dihitung dari persentase kehilangan berat dan derajat serangan rayap pada kayu menurut SNI 01-7207-2006 tentang klasifikasi ketahanan kayu terhadap serangan rayap tanah. Berdasarkan hasil penelitian disimpulkan bahwa penggunaan bahan pengawet akar tuba getah susu dan akar tuba getah bening dengan konsentrasi konsentrasi 2% dan 4%, dinilai belum efektif untuk di jadikan bahan mengawetkan kayu Gmelina dari serangan Rayap Tanah. Kata kunci: Kayu Gmelina, pengawetan, akar tuba getah susu, akar tuba getah bening, rayap tanah, Kehilangan berat, derajat serangan.
Mitophagy and Neurodegeneration: Between the Knowns and the Unknowns
Authors: Jetto Cuckoo Teresa , Nambiar Akshaya , Manjithaya Ravi 
Published: March 2022

Macroautophagy (henceforth autophagy) an evolutionary conserved intracellular pathway, involves lysosomal degradation of damaged and superfluous cytosolic contents to maintain cellular homeostasis. While autophagy was initially perceived as a bulk degradation process, a surfeit of studies in the last 2 decades has revealed that it can also be selective in choosing intracellular constituents for degradation. In addition to the core autophagy machinery, these selective autophagy pathways comprise of distinct molecular players that are involved in the capture of specific cargoes. The diverse organelles that are degraded by selective autophagy pathways are endoplasmic reticulum (ERphagy), lysosomes (lysophagy), mitochondria (mitophagy), Golgi apparatus (Golgiphagy), peroxisomes (pexophagy) and nucleus (nucleophagy). Among these, the main focus of this review is on the selective autophagic pathway involved in mitochondrial turnover called mitophagy. The mitophagy pathway encompasses diverse mechanisms involving a complex interplay of a multitude of proteins that confers the selective recognition of damaged mitochondria and their targeting to degradationviaautophagy. Mitophagy is triggered by cues that signal the mitochondrial damage such as disturbances in mitochondrial fission-fusion dynamics, mitochondrial membrane depolarisation, enhanced ROS production, mtDNA damage as well as developmental cues such as erythrocyte maturation, removal of paternal mitochondria, cardiomyocyte maturation and somatic cell reprogramming. As research on the mechanistic aspects of this complex pathway is progressing, emerging roles of new players such as the NIPSNAP proteins, Miro proteins and ER-Mitochondria contact sites (ERMES) are being explored. Although diverse aspects of this pathway are being investigated in depth, several outstanding questions such as distinct molecular players of basal mitophagy, selective dominance of a particular mitophagy adapter protein over the other in a given physiological condition, molecular mechanism of how specific disease mutations affect this pathway remain to be addressed. In this review, we aim to give an overview with special emphasis on molecular and signalling pathways of mitophagy and its dysregulation in neurodegenerative disorders.
Nintedanib and Dasatinib Treatments Induce Protective Autophagy as a Potential Resistance Mechanism in MPM Cells
Authors: Hegedüs Luca , Szücs Kata D. , Kudla Matthias , Heidenreich Julian , Jendrossek Verena , Peña-Llopis Samuel , Garay Tamas , Czirok Andras , Aigner Clemens , Plönes Till , Vega-Rubin-de-Celis Silvia , Hegedüs Balazs 
Published: March 2022

Malignant pleural mesothelioma (MPM) is a rare type of cancer with a grim prognosis. So far, no targetable oncogenic mutation was identified in MPM and biomarkers with predictive value toward drug sensitivity or resistance are also lacking. Nintedanib (BIBF1120) is a small-molecule tyrosine kinase inhibitor that showed promising efficacy preclinically and in phase II trial in MPM as an angiogenesis inhibitor combined with chemotherapy. However, the extended phase III trial failed. In this study, we investigated the effect of nintedanib on one of its targets, the SRC kinase, in two commercial and six novel MPM cell lines. Surprisingly, nintedanib treatment did not inhibit SRC activation in MPM cells and even increased phosphorylation of SRC in several cell lines. Combination treatment with the SRC inhibitor dasatinib could reverse this effect in all cell lines, however, the cellular response was dependent on the drug sensitivity of the cells. In 2 cell lines, with high sensitivity to both nintedanib and dasatinib, the drug combination had no synergistic effect but cell death was initiated. In 2 cell lines insensitive to nintedanib combination treatment reduced cell viability synergisticaly without cell death. In contrast, in these cells both treatments increased the autophagic flux assessed by degradation of the autophagy substrate p62 and increased presence of LC3B-II, increased number of GFP-LC3 puncta and decreased readings of the HiBiT-LC3 reporter. Additionaly, autophagy was synergistically promoted by the combined treatment. At the transcriptional level, analysis of lysosomal biogenesis regulator Transcription Factor EB (TFEB) showed that in all cell lines treated with nintedanib and to a lesser extent, with dasatinib, it became dephosphorylated and accumulated in the nucleus. Interestingly, the expression of certain known TFEB target genes implicated in autophagy or lysosomal biogenesis were significantly modified only in 1 cell line. Finally, we showed that autophagy induction in our MPM cell lines panel by nintedanib and dasatinib is independent of the AKT/mTOR and the ERK pathways. Our study reveals that autophagy can serve as a cytoprotective mechanism following nintedanib or dasatinib treatments in MPM cells.
Assessment of Renal Risk Score and Histopathological Classification for Prediction of End-Stage Kidney Disease and Factors Associated With Change in eGFR After ANCA-Glomerulonephritis Diagnosis
Authors: Brilland Benoit , Boud’hors Charlotte , Copin Marie-Christine , Jourdain Pierre , Henry Nicolas , Wacrenier Samuel , Djema Assia , Samoreau Clément , Coindre Jean-Philippe , Cousin Maud , Riou Jeremie , Croue Anne , Saint-André Jean-Paul , Subra Jean-François , Piccoli Giorgina Barbara , Augusto Jean-François 
Published: March 2022

IntroductionThe “Renal Risk Score” (RRS) and the histopathological classification have been proposed to predict the risk of end-stage kidney disease (ESKD) in ANCA-associated glomerulonephritis (ANCA-GN). Besides, factors associated with kidney function recovery after ANCA-GN onset remain to be more extensively studied. In the present study, we analyzed the value of the RRS and of the histopathological classification for ESKD prediction. Next, we analyzed factors associated with eGFR change within the first 2 years following ANCA-GN diagnosis.Materials and MethodsWe included patients from the Maine–Anjou ANCA-associated vasculitis registry with at least 6 months of follow-up. The values of ANCA-GN, histopathological classification, and RRS, and the factors associated with eGFR variations between ANCA-GN diagnosis and 2 years of follow-up were assessed.ResultsThe predictive values of the histopathological classification and RRS were analyzed in 123 patients. After a median follow-up of 42 months, 33.3% patients developed ESKD. The predictive value of RRS for ESKD was greater than that of the histopathological classification. Determinants of eGFR variation were assessed in 80/123 patients with complete eGFR measurement. The median eGFR increased from ANCA-GN diagnosis to month 6 and stabilized thereafter. The only factor associated with eGFR variation in our study was eGFR at ANCA-GN diagnosis, with higher eGFR at diagnosis being associated with eGFR loss (p&lt;0.001).ConclusionThe RRS has a better predictive value for ESKD than the histopathological classification. The main determinant of eGFR variation at 2 years was eGFR at ANCA-GN diagnosis. Thus, this study suggests that eGFR recovery is poorly predicted by histological damage at ANCA-GN diagnosis.
“The Stakes Are Higher”- Patient and Caregiver Perspectives on Cystic Fibrosis Research and Personalized Medicine
Authors: Knoppers Terese , Cosquer Marie , Hagan Julie , Nguyen Minh Thu , Knoppers Bartha Maria 
Published: March 2022

IntroductionMaking bench to bedside advances in cystic fibrosis (CF) care requires the sustained engagement and trust of people living with CF. However, there is a scarcity of studies exploring their concerns and priorities regarding research and its end products. The aim of this qualitative study was to generate empirical evidence regarding patient and caregiver perspectives on cystic fibrosis research and personalized medicine to foster developments in translational research in Canada.MethodsA total of 15 focus groups were conducted, engaging 22 adults with CF and 18 caregivers (e.g., parents, siblings and partners) living in Canada. Inductive thematic analysis relied on an iterative process involving themes derived from both participant meaning-making and existing scientific literature. Participant perspectives were considered along intrapersonal, intracommunity, interpersonal, and structural lines.ResultsOverall, participants described a relationship to CF research inextricable from the lived experience of CF as a lifelong progressive and terminal disease and from the goal of advancing medical science. They were enthusiastic and excited about the emergence of CFTR modulators, although they had some knowledge gaps regarding the associated research. They largely spoke to positive experiences with researcher communication but had feedback regarding informed consent processes and the return of study results. Participants also voiced concerns about structural access barriers to research and to its end products. Extensive histories of research participation, a relatively small and intercommunicative CF community, and structural overlap between research and care settings contributed to their perspectives and priorities.ConclusionStudy findings are valuable for researchers and policy-makers in CF and rare or progressive diseases more broadly. Continuing to solicit and listen to the voices of patients and caregivers is crucial for research ethics and the translation of new therapies in the area of personalized medicine.
Analysis of Long-Term Cochlear Implantation Outcomes and Correlation With Imaging Characteristics in Patients With Common Cavity Deformity
Authors: Zhang Lifang , Chen Biao , Kong Ying , Liau Natalia , Wei Xingmei , Shi Ying , Chen Jingyuan , Yang Mengge , Dhanasingh Anandhan , Li Yongxin 
Published: March 2022

ObjectTo investigate the long-term development of auditory and speech in patients with common cavity deformity (CCD) after cochlear implantation (CI) and its relationship to imaging characteristics.MethodsTwenty-three CCD patients and 59 age- and sex-matched CI children with normal inner ear structure were recruited. The auditory and speech development of these two groups were evaluated at 0, 1, 3, 6, 12, and 18 months after CI activation using four parent reports questionnaires [Categories of Auditory Performance (CAP), Speech Intelligibility Rating (SIR), Meaningful Auditory Integration Scale/Infant-Toddler Meaningful Auditory Integration Scale (MAIS/ITMAIS), and Meaningful Use of Speech Scale (MUSS)]. Computed tomography-based 3-dimensional reconstruction of the surgical side of 18 CCD children was performed, the volume and surface area were calculated. Correlation analysis was performed on the imaging performance and post-operative outcomes.ResultsThe percentages of MAIS/IT-MAIS scores and CAP scores at different evaluation time points are significantly different (p &lt; 0.05). When comparing SIR results across time points, significant growth was observed in most of the comparisons. In addition, significant differences (p &lt; 0.05) are observed among the percentages of MUSS scores at different time points except the comparison between 0 and 1 month after CI activation. Patients in the CCD group had poorer auditory and speech performances at different stages after CI compared with those in the control group. According to the reconstruction of CCD patients, the volume ranged from 12.21 to 291.96 mm3; the surface area ranged from 27.81 to 284.7 mm2. When the lumen surface area was &lt;190.45 mm2 or the volume was &lt;157.91 mm3, the survival time for CCD children to achieve a CAP score of 4 after CI was significantly shorter.ConclusionCochlear implantation are less effective in CCD patients than in patients with normal inner ear structures, but they can still achieve significant improvement post-operatively. The morphology and size of the inner ear vary in CCD patients, which reflects the degree of inner ear development influences the outcome after CI surgery.
Preoperative Prediction of Central Cervical Lymph Node Metastasis in Fine-Needle Aspiration Reporting Suspicious Papillary Thyroid Cancer or Papillary Thyroid Cancer Without Lateral Neck Metastasis
Authors: Zhang Kai , Qian Lang , Chen Jieying , Zhu Qian , Chang Cai 
Published: March 2022

PurposeNo non-invasive method can accurately determine the presence of central cervical lymph node (CCLN) metastasis in papillary thyroid cancer (PTC) until now. This study aimed to investigate factors significantly associated with CCLN metastasis and then develop a model to preoperatively predict CCLN metastasis in fine-needle aspiration (FNA) reporting suspicious papillary thyroid cancer (PTC) or PTC without lateral neck metastasis.Patients and MethodsConsecutive inpatients who were diagnosed as suspicious PTC or PTC in FNA and underwent partial or total thyroidectomy and CCLN dissection between May 1st, 2016 and June 30th, 2018 were included. The total eligible patients were randomly divided into a training set and an internal validation set with the ratio of 7:3. Univariate analysis and multivariate analysis were conducted in the training set to investigate factors associated with CCLN metastasis. The predicting model was built with factors significantly correlated with CCLN metastasis and validated in the validation set.ResultsA total of 770 patients were eligible in this study. Among them, 268 patients had histologically confirmed CCLN metastasis, while the remaining patients did not. Factors including age, BRAF mutation, multifocality, size, and capsule involvement were found to be significantly correlated with the CCLN metastasis in univariate and multivariate analysis. A model used to predict the presence CCLN metastasis based on these factors and US CCLN status yielded AUC, sensitivity, specificity and accuracy of 0.933 (95%CI: 0.905-0.960, p &lt; 0.001), 0.816, 0.966 and 0.914 in the training set and 0.967 (95%CI: 0.943-0.991, p &lt; 0.001), 0.897, 0.959 and 0.936 in the internal validation set.ConclusionAge, BRAF mutation, multifocality, size, and capsule involvement were independent predictors of CCLN metastasis in FNA reporting suspicious PTC or PTC without lateral neck metastasis. A simple model was successfully built and showed excellent discrimination to distinguish patients with or without CCLN metastasis.
ERBB2 Mutations as Potential Predictors for Recurrence in Colorectal Serrated Polyps by Targeted Next-Generation Sequencing
Authors: Wang Qi-Wen , Wang Xin-Yuan , Zhang Qing-Wei , Chen Jin-Nan , Zhou Yu-Jie , Tang Zhao-Rong , Wang Rui-Lan , Chen Haoyan , Chen Huimin , Li Xiao-Bo 
Published: March 2022

BackgroundFollow-up guidelines for serrated polyps (SPs) are mainly based on factors such as histology and size with limited evidence. The underlying genomic mechanism of SPs in relation to recurrence risks is utterly unknown.MethodsWe applied targeted next-generation sequencing (NGS) approach on two groups of SPs [polyp-relapsed SPs (PRSPs) vs. polyp-free SPs (PFSPs)] based on the surveillance outcomes to compare differences of DNA variants in 71 colorectal cancer-associated genes. A multicenter validation cohort was established longitudinally from 2016 to 2019 to confirm the relevant results.ResultsAmong the 96 NGS samples, at least one mutant after filtration was detected in 90 samples (94%). Molecular profiling presented BRAF, KRAS, and APC as top 3 mutated genes. FBXW7, MSH2, and ERBB2 might be recurrence-relevant, while DMD, BRCA1, and BRCA2 might be negatively correlated with recurrence. Notably, ERBB2 mutants (R678Q and V842I) (n = 5) had higher risks of polyp recurrence than the wild types (n = 85), with a median polyp-free interval of 15 months compared to 26 months [P &lt; 0.001; hazard ratio (HR) = 4.9; 95% confidence interval (CI) = 1.9–12.8]. Furthermore, a multicenter cohort composed by 321 SPs verified that ERBB2-mutated SPs had increased risks of polyp recurrence (P &lt; 0.001; HR = 3.7; 95% CI = 2.3–6.0) and advanced neoplastic lesion (ANL) recurrence (P &lt; 0.001; HR = 10.0; 95% CI = 2.7–36.9) compared with wild-type SPs, respectively.ConclusionsOur results are emphasizing that SP individuals with ERBB2 mutants are at higher risks of subsequent colorectal neoplasms. ERBB2 mutants might work as facilitated markers for prediction of high-risk SPs and might implicate a potential mechanism in the serrated pathway to colorectal carcinoma (CRC).