bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using tenets from the Bhagavad Gita in counselling diaspora Hindu older adults: Impact on stress mitigation and improving quality of life
Authors: Pandya Samta P. 
Published: August 2022

AbstractThis article reports a study on the impact of a Bhagavad Gita (BG) text‐based counselling programme for long‐term immigrant diaspora Hindu older adults in the US and UK. Compared with the journal writing sessions, participants of the BG lessons reported lower stress and better quality of life post‐test (p ≤ 0.025; Hedges' g = −0.65–0.36). Results of the latent class analyses highlighted complex behaviour patterns in the post‐test dataset and foregrounded six classes/subgroups of participants likely to gain most from the BG programme: older adult females, diaspora Hindu older adults with postgraduate‐professional qualifications, widowed older adults, those living alone or with other kith‐kin, those with above threshold programme compliance (defined as those who attended 13–24 (>50%) BG lessons, and completed 13–24 (>50%) BG homework lessons). Overall, the results of the study endorse the connection between age and religiosity and support a BG text‐based counselling programme as a workable strategy for long‐term immigrant diaspora Hindu older adults. The BG text provides a firm ethnoculturally grounded counselling approach to work with diaspora Hindu older adult clients, taking cognisance of their intersectional realities. It may help them cope with the dynamics of the modern, traditional, and continuously shifting realities in a foreign milieu.
Rational Control of Off‐State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement**
Authors: Adam Virgile , Hadjidemetriou Kyprianos , Jensen Nickels , Shoeman Robert L. , Woodhouse Joyce , Aquila Andrew , Banneville Anne‐Sophie , Barends Thomas R. M. , Bezchastnov Victor , Boutet Sébastien , Byrdin Martin , Cammarata Marco , Carbajo Sergio , Eleni Christou Nina , Coquelle Nicolas , De la Mora Eugenio , El Khatib Mariam , Moreno Chicano Tadeo , Bruce Doak R. , Fieschi Franck , Foucar Lutz , Glushonkov Oleksandr , Gorel Alexander , Grünbein Marie Luise , Hilpert Mario , Hunter Mark , Kloos Marco , Koglin Jason E. , Lane Thomas J. , Liang Mengning , Mantovanelli Angela , Nass Karol , Nass Kovacs Gabriela , Owada Shigeki , Roome Christopher M. , Schirò Giorgio , Seaberg Matthew , Stricker Miriam , Thépaut Michel , Tono Kensuke , Ueda Kiyoshi , Uriarte Lucas M. , You Daehyun , Zala Ninon , Domratcheva Tatiana , Jakobs Stefan , Sliwa Michel , Schlichting Ilme , Colletier Jacques‐Philippe , Bourgeois Dominique , Weik Martin 
Published: August 2022

AbstractReversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance and novel applications. Here we establish a link between photoswitching contrast, one of the key parameters that dictate the achievable resolution in nanoscopy applications, and chromophore conformation in the non‐fluorescent state of rsEGFP2, a widely employed label in REversible Saturable OpticaL Fluorescence Transitions (RESOLFT) microscopy. Upon illumination, the cis chromophore of rsEGFP2 isomerizes to two distinct off‐state conformations, trans1 and trans2, located on either side of the V151 side chain. Reducing or enlarging the side chain at this position (V151A and V151L variants) leads to single off‐state conformations that exhibit higher and lower switching contrast, respectively, compared to the rsEGFP2 parent. The combination of structural information obtained by serial femtosecond crystallography with high‐level quantum chemical calculations and with spectroscopic and photophysical data determined in vitro suggests that the changes in switching contrast arise from blue‐ and red‐shifts of the absorption bands associated to trans1 and trans2, respectively. Thus, due to elimination of trans2, the V151A variants of rsEGFP2 and its superfolding variant rsFolder2 display a more than two‐fold higher switching contrast than their respective parent proteins, both in vitro and in E. coli cells. The application of the rsFolder2‐V151A variant is demonstrated in RESOLFT nanoscopy. Our study rationalizes the connection between structural and photophysical chromophore properties and suggests a means to rationally improve fluorescent proteins for nanoscopy applications.
Comparative Analysis of a Series of pH‐Responsive Sulphonated Europium Complexes for Bioassays Monitoring Acidification
Authors: Fradgley Jack D. , Starck Matthieu , Lamarque Laurent , Parker David 
Published: August 2022

AbstractThe synthesis and photophysical evaluation of a series of hydrophilic sulphonated and pH responsive luminescent europium(III) probes are reported. The Eu emission intensities and lifetimes increase very significantly on lowering pH, leading to a ‘switching on’ of Eu luminescence in more acidic media. By varying the nature of the substituents at an integral aniline nitrogen atom located in the sensitising chromophore, the pKa value of the complex can be tuned to between 4.3 and 6.3 for a set of five Eu(III) complexes. These systems are designed to allow conjugation to targeting vectors permitting, for example, the monitoring of receptor internalisation in cells. This approach can be used to tag proteins selectively and allows the monitoring of uptake into acidic organelles, enabling the creation of time‐resolved internalisation assays to follow pH change in real time, both in vitro and in cellulo.
A Concept Formula With Large, Milk Phospholipid‐Coated Lipid Droplets Enriched With Milk Fat Decreases Palmitic Acid and Calcium Levels in Stools of Healthy, Term Infants
Authors: van de Heijning Bert J. M. , Schoen Stefanie , Abrahamse‐Berkeveld Marieke , Hokken‐Koelega Anita C. S. , van der Beek Eline M. 
Published: August 2022

AbstractThis study evaluates the impact of an infant formula comprising large, dairy milk phospholipid‐coated lipid droplets containing a vegetable‐dairy lipid mixture (Concept‐Mix) with 6% of fatty acid (FA) as sn‐2 palmitic acid (PA) on infant stool characteristics. Stool samples from infants fed Concept‐Mix or standard formula with 97% vegetable lipids with 2% of FA as sn‐2 PA (Control‐Veg) are evaluated at baseline, 13 and 52 weeks of age. Overall, mean stool fat content decreases from 8 to 10 w% at baseline to <2 w% at 52 weeks. At 13 weeks, PA and Calcium (Ca) content, as well as FA sum are lower in Concept‐Mix than Control‐Veg stools, with values closer to breastfed infants. The increased dietary sn‐2 PA content is regarded as instrumental for this fecal PA reduction. Indeed, in their proof of principle study in rats fed formula‐based diets using Control‐Veg, Concept‐Mix, or Concept formula with 95% vegetable lipids (Concept‐Veg) comprising 2%, 6%, and 2% of FA as sn‐2 PA, they demonstrate that PA absorption is the highest for Concept‐Mix. Hence, a concept formula with large, milk phospholipid coated lipid droplets enriched with milk fat beneficially affects PA and Ca absorption in infants, likely due to its increased sn‐2 PA content.Practical applications: The results of these studies show the merit with regard to sn‐2 palmitic acid (PA) and Calcium absorption of increasing the sn‐2 PA content in the fat blend of infant formula by including dairy milk fat.
Harmol hydrochloride dihydrate induces autophagy in neuro cells and promotes the degradation of α‐Syn by Atg5/Atg12‐dependent pathway
Authors: Abulimiti Gulinuer , Zeng Jianghua , Aimaiti Mutalifu , Lei Xiuying , Mi Na 
Published: August 2022

AbstractHarmol hydrochloride dihydrate (HHD) is a novel alkaloid salt of the natural β‐carboline harmol, which is isolated from Peganum harmala L. Here, we studied whether HHD could induce autophagy in neuro cells and investigated the underlying molecular mechanism. After incubation with HHD, the number of GFP‐LC3 puncta in cells was measured using confocal microscopy. The distribution and colocalization of autophagosomes and autolysosomes in the cells were also detected. LC3 was gathered and cultured in a medium containing HHD. Compared with control cells and cells starved for 2 h, the number of GFP‐LC3 puncta and the LC3‐II expression level were significantly increased in HHD‐treated cells (p < .05). The number of autophagosome (red) was increased and most of them were colocalized with lysosomes (green). Moreover, HHD induced the formation of puncta with Lysotracker Red positive in the L3 fat bodies (p < .05). When treated HEK cells with HHD, the protein expression level of LC3‐II was markedly increased, and the protein expression level of α‐Syn was significantly decreased (p < .05). HHD could induce the increased autophagosome in neuro cells by induction of autophagy. Moreover, HHD may promote the degradation of α‐Syn protein to protect neuro cells by inducing autophagy.
History of Chemical Notations from Alchemy to Psycho‐Chemistry
Authors: Marx Gerard , Gilon Chaim 
Published: August 2022

AbstractWriting involves the transformation of ideas, emotions and sounds into graphic form. Ideagrams help clarify complex ideas and can be considered to be forms of stored memory. The emergence of chemistry from alchemy involved the adaptation of graphic notations to comprehend the invisible atomic and molecular structures of complex matter, and to spread and store that knowledge. This culminated in the molecular representations of chemical reactions and chemical compounds such as natural products, sugars, polymers, proteins and metal complexes. We review the development of textual and graphic notations of chemical structures from circa‐1700 to now. We present a Timeline that graphically summarizes the salient stages in the evolution of chemical notations (“chemography”) from alchemy, leading to modern “psycho‐chemistry”. The Timeline correlates the emergence of new chemical notations with crucial technological inventions.
Gold nanoparticle decorated blu‐ray digital versatile disc as a highly reproducible surface‐enhanced Raman scattering substrate for detection and analysis of rotavirus RNA in laboratory environment
Authors: Biswas Sritam , Devi Yengkhom Damayanti , Sarma Dipjyoti , Namsa Nima D. , Nath Pabitra 
Published: August 2022

AbstractDetection and estimation of various biomolecular samples are often required in research and clinical laboratory applications. Present work demonstrates the functioning of a surface‐enhanced Raman scattering (SERS) substrate that has been obtained by drop‐casting of citrate‐reduced gold nanoparticles (AuNPs) of average dimension of 23 nm on a bare blu‐ray digital versatile disc (BR‐DVD) substrate. The performance of the proposed SERS substrate has been initially evaluated with standard Raman active samples, namely malachite green (MG) and 1,2‐bis(4‐pyridyl)ethylene (BPE). The designed SERS substrate yields an average enhancement factor of 3.2 × 106 while maintaining reproducibility characteristics as good as 94% over the sensing region of the substrate. The usability of the designed SERS substrate has been demonstrated through the detection and analysis of purified rotavirus double‐stranded RNA (dsRNA) samples in the laboratory environment condition. Rotavirus RNA concentrations as low as 10 ng/μL could be detected with the proposed sensing scheme.
Maintenance of increased bone mass after PTH withdrawal by sequential medicarpin treatment via augmentation of cAMP‐PKA pathway
Authors: Sharma Kriti , Awasthi Pallavi , Prakash Ravi , Khanka Sonu , Bajpai Ranju , Sahasrabuddhe Amogh A. , Goel Atul , Singh Divya 
Published: August 2022

AbstractOsteoporosis is a metabolic bone disorder associated with impaired bone microarchitecture leading to fragility fractures. Long‐term usage of parathyroid hormone (PTH) enhances bone resorption and leads to osteosarcoma in rats which limits its exposure to maximum 2 years in human. Notably, the anabolic effects of PTH do not endure in the absence of sustained administration. Studies in our lab identified osteogenic and antiresorptive activity in medicarpin, a phytoestrogen belonging to the pterocarpan class. Considering dual‐acting property of medicarpin and limitations of PTH therapy, we envisaged that medicarpin sequential treatment after PTH withdrawal could serve as promising therapeutic approach for osteoporosis treatment. As PTH exerts its bone anabolic effect by increasing osteoblast survival, our study aims to determine whether medicarpin amplifies this effect of PTH. Our results show that PTH withdrawal led to reduced bone mineral density and bone parameters, while sequential treatment of medicarpin after PTH withdrawal significantly enhanced these parameters. Remarkably, these effects were more pronounced than 8‐week PTH treatment. Sequential therapy also significantly increased P1NP levels and decreased CTX levels and TRAP positive cells compared to PTH 8W group where CTX levels were quite high due to bone resorptive action of PTH. Protein expression studies revealed that medicarpin along with PTH betters the antiapoptotic potential compared to PTH alone, through augmentation of cyclic adenosine monophosphate‐PKA‐CREB pathway. These results proclaim that medicarpin sequential treatment prevented the reduction in bone accrual and strength accompanying PTH withdrawal and also aided in antiapoptotic role of PTH. The study points toward the potential use of medicarpin as a replacement therapeutic option postdiscontinuation of PTH.
The role of endoplasmic reticulum stress in the regulation of long noncoding RNAs in cancer
Authors: Ebrahimi Nasim , Saremi Jamileh , Ghanaatian Masoud , Yazdani Elnaz , Adelian Samaneh , Samsami Sahar , Moradi Neda , Rostami Ravari Nadi , Ahmadi Amirhossein , Hamblin Michael R. , Aref Amir Reza 
Published: August 2022

AbstractCancer cells must overcome a variety of external and internal stresses to survive and proliferate. These unfavorable conditions include the accumulation of mutations, nutrient deficiency, oxidative stress, and hypoxia. These stresses can cause aggregation of misfolded proteins inside the endoplasmic reticulum. Under these conditions, the cell undergoes endoplasmic reticulum stress (ER‐stress), and consequently initiates the unfolded protein response (UPR). Activation of the UPR triggers transcription factors and regulatory factors, including long noncoding RNAs (lncRNAs), which control the gene expression profile to maintain cellular stability and hemostasis. Recent investigations have shown that cancer cells can ensure their survival under adverse conditions by the UPR affecting the expression of lncRNAs. Therefore, understanding the relationship between lncRNA expression and ER stress could open new avenues, and suggest potential therapies to treat various types of cancer.
Completion of postoperative polysomnography for children with severe obstructive sleep apnea: A quality improvement project
Authors: Jiang Wen , Bhattacharjee Rakesh , Nation Javan , Brigger Matthew T. 
Published: August 2022

AbstractObjectivePediatric patients with severe obstructive sleep apnea (OSA) are at risk for residual OSA following tonsillectomy with/without adenoidectomy (T ± A). We initiated a quality improvement (QI) project to increase the percentage of postoperative (postop) polysomnography (PSG) completion to identify residual OSA.MethodsThis is a prospective QI project carried out at a tertiary pediatric academic hospital. Children ≤18 years of age who underwent T ± A for severe OSA were included. Our Specific, Measurable, Attainable, Relevant, and Time‐based (SMART) aim was to increase the percentage of completed postop PSGs in this cohort from a baseline of 70% to95% by May 31, 2021. We focused on patient education and leveraged both clinical decision support and reporting functionalities of the electronic medical record for project implementation.ResultsDuring the pre‐intervention period between January 1, 2019 to June 30, 2020, 472 patients met the inclusion criteria with an average age of 8.6 years (SD 4.6). The rate of postop PSG completion was 69.7% (SD 11.4%) with an average time of 99 days (SD 66) between surgery and the postop PSG. A shift was observed starting in September 2020, and the PSG completion rate improved to 94.9% by September 30, 2021. Post‐intervention, there were 178 patients with an average age of 9.3 years (SD 4.9). The average time between surgery and the postop PSG was significantly reduced to 57 days (SD 16; p < .001).ConclusionsThrough a multidisciplinary approach, we successfully completed our SMART aim. With the establishment of QI infrastructure, our goal is to deliver better care in a sustainable fashion using QI methodology.
Opportunities to Foster Conservation and Sustainable Use of Biodiversity Beyond National Jurisdiction: A Role for Scientists
Authors: Muraki Gottlieb Hiroko , Girguis Peter R. 
Published: August 2022

AbstractCountries are currently negotiating a comprehensive global binding treaty at the United Nations to better protect biodiversity in the high seas (referred to from here as “the Treaty”). Developing a governance framework for the sharing of benefits from marine genetic resources (MGRs) of the high seas (or areas beyond national jurisdiction, referred to from here as “ABNJ”) is a crucial element of the proposed treaty. Negotiating countries' positions on MGRs vary widely, ranging from no regulation to the establishment of a new global regulatory mechanism. For the scientists who may be involved with collecting MGRs in ABNJ, the Treaty could impact their research activities, including mandatory precollection notification. There could also be new requirements for daily research activities associated with utilizing physical materials collected in ABNJ or data generated from such materials. For indigenous peoples and local communities (IPLC) that may hold relevant traditional knowledge, the Treaty could ensure their rights to prior, free, and informed consent and the use of mutually agreed terms. The scientific community and IPLC have an opportunity to engage with key stakeholders to increase the chances that any new requirements will be practical, remain relevant in decades to come, and provide a platform that will foster research, collaboration, and marine biological diversity conservation.
A novel automatic‐dependent surveillance‐broadcast patch antenna for aircraft tracking
Authors: Aguni Lahcen , Chabaa Samira , Ibnyaich Saida , Sellak Lahcen , Sarosh Ahmad , Zeroual Abdelouhab 
Published: August 2022

AbstractThis paper focuses on the design of a novel automatic‐dependent surveillance‐broadcast (ADS‐B) patch antenna operating at 1090 MHz with good bandwidth and good gain for aircraft tracking and navigation purposes. The structure of the proposed antenna consists of a long strip line ended by a crescent patch, a U‐shaped ground plane, and a rectangular substrate. The proposed antenna is simulated using both HFSS and CST electromagnetic simulators. The overall dimensions of the antenna are 130 × 26 × 1.6 mm3, and is fabricated on a low‐cost FR‐4 epoxy substrate. Various parameters are determined and analyzed to evaluate the antenna performance in terms of reflection coefficient, impedance bandwidth, radiation pattern, gain, and efficiency. It has been verified and tested that the simulated results are in very good agreement with the measured ones. At the desired frequency of 1090 MHz, the ADS‐B patch antenna has a gain of 2.23 dBi and a measured impedance bandwidth at −10 dB of 200 MHz. The antenna has an omnidirectional radiation pattern, which will allow the antenna to pick up ADS‐B signals broadcasted by aircraft in all directions. An ADS‐B system has been built and validated using aircraft data provided by the Flightradar platform.
Synthesis of syndiotactic polystyrene—Polyisobutylene graft copolymers by cationic half‐sandwich scandium complex
Authors: Yang Ke , Wang Shanda , Zhou Rifeng , Cheng Qingli , Wang Quanguo , Li Yang 
Published: August 2022

AbstractSyndiotactic polystyrene is a high strength engineering plastic, and polyisobutylene is an excellent elastomers. In order to combine the advantages of syndiotactic polystyrene and polyisobutylene, isobutylene was achieved to graft onto the crystalline syndiotactic polystyrene, forming graft copolymers, under half sandwich scandium complex [(C5Me4SiMe3)Sc(CH2SiMe3)2THF]/[Ph3C][B(C6F5)4] after the application of syndiotactic copolymers of styrene and 4‐isopropenylstyrene with pendant double bonds. Grafted copolymers based on crystalline polystyrene and rubbery polyisobutylene block have both high Tm (270°C) and low Tg (−67°C), meanwhile, these materials have relative good mechanical properties.
Establishing construct validity of neuropsychological tests in cancer survivors
Authors: Treviño Melissa , Zhu Xiaoshu , Lu Yi Yi , Scheuer Luke Sanders , Passell Eliza , Huang Grace , Germine Laura Thi , Horowitz Todd Steven 
Published: August 2022

AbstractObjectiveCancer‐related cognitive impairments (CRCI) are frequently reported among cancer survivors, and attention is the most frequently assessed cognitive domain in CRCI. However, there is no consensus as to whether attention is impaired. We suggest that a major reason for this lack of agreement is a lack of construct validity for neuropsychological attention tests. We propose to assess the construct validity of neuropsychological attention tests with respect to experimental paradigms from cognitive psychology.MethodsSelf‐reported cancer survivors (N = 314) completed an online battery comprising six experimental attention paradigms and eight neuropsychological tests. Confirmatory factor analysis was used to evaluate the fit of five models derived from a general population sample (N = 636) in a previous study (M. Treviño, Cogn Res Princ Implic, in press). We then subjected the best‐fitting model to a measurement invariance analysis.ResultsThe best‐fitting model was a six intercorrelated factor structure, comprising Capacity, Search, Digit Span, Arithmetic, Sustained Attention, and Flanker Interference factors. Configural and weak invariance held, indicating that the factor loadings were invariant across groups. Strong invariance, indicating that intercepts were also invariant, held except for the Approximate Number Sense test.ConclusionsAccording to our factor model, Spatial Span and Digit Symbol Coding measure attentional capacity, while the Trail Making Test (A&B) and Letter Cancellation tests measure visual search ability. However, Digit Span and Arithmetic tests do not measure attention. We hope that these results will lead to better scientific models, better patient education, and, ultimately, improved outcomes for survivors.