bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA CEBPA-DT promotes liver cancer metastasis through DDR2/β-catenin activation via interacting with hnRNPC
Authors: Cai Yunshi , Lyu Tao , Li Hui , Liu Chang , Xie Kunlin , Xu Lin , Li Wei , Liu Hu , Zhu Jiang , Lyu Yinghao , Feng Xuping , Lan Tian , Yang Jiayin , Wu Hong 
Published: September 2022

AbstractBackground Hepatocellular carcinoma (HCC) is the world’s third leading cause of cancer-related death; due to the fast growth and high prevalence of tumor recurrence, the prognosis of HCC patients remains dismal. Long non-coding RNA CEBPA-DT, a divergent transcript of the CCAAT Enhancer Binding Protein Alpha (CEBPA) gene, has been shown to participate in multiple tumor progression. However, no research has established its cancer-promoting mechanism in HCC yet. Methods CEBPA-DT was identified in human HCC tissues through RNA sequencing. The expression level of CEBPA-DT was assessed by quantitative real-time PCR. The biological effects of CEBPA-DT were evaluatedin vitroandin vivothrough gain or loss of function experiments. RNA fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP) and RNA pull-down assays were applied to investigate the downstream target of CEBPA-DT. Immunofluorescence, subcellular protein fractionation, western blot, and co-immunoprecipitation were performed to analyze the subcellular location of β-catenin and its interaction with Discoidin domain-containing receptor 2 (DDR2). Results CEBPA-DT was upregulated in human HCC tissues with postoperative distant metastasis and intimately related to the worse prognosis of HCC patients. Silencing of CEBPA-DT inhibited the growth, migration and invasion of hepatoma cellsin vitroandin vivo, while enhancement of CEBPA-DT played a contrasting role. Mechanistic investigations demonstrated that CEBPA-DT could bind to heterogeneous nuclear ribonucleoprotein C (hnRNPC), which facilitated cytoplasmic translocation of hnRNPC, enhanced the interaction between hnRNPC and DDR2 mRNA, subsequently promoted the expression of DDR2. Meanwhile, CEBPA-DT induced epithelial-mesenchymal transition (EMT) process through upregulation of Snail1 via facilitating nuclear translocation of β-catenin. Using DDR2 inhibitor, we revealed that the CEBPA-DT induced the interaction between DDR2 and β-catenin, thus promoting the nuclear translocation of β-catenin to activate transcription of Snail1, contributing to EMT and HCC metastasis. Conclusions Our results suggested that CEBPA-DT promoted HCC metastasis through DDR2/β-catenin mediated activation of Snail1 via interaction with hnRNPC, indicating that the CEBPA-DT-hnRNPC-DDR2/β-catenin axis may be used as a potential therapeutic target for HCC treatment.
Using crossed random-effects models to test the effects of None of the above and personality traits in multiple-choice items
Authors: Sanz Susana , García Carmen , Olmos Ricardo 
Published: September 2022

Abstract

Introduction: Including general options on items like None-of-the-above (NOTA) could lead to worse psychometric properties. Further, personality traits are related to academic performance and could influence NOTA effects. Therefore, this study aims to test the effects of NOTA by manipulating its appearance, its use as the correct option, or as a distractor considering the examinees’ personality traits.
Method: The sample consisted of 449 psychology students that answered a statistics concept inventory. A crossed random-effects model was conducted to model the probability of answering an item correctly depending on items and students’ covariates.
Results: The appearance of NOTA negatively affects the probability to answer correctly, and changes between examinees. Conscientiousness, Extraversion, Emotional Stability, and previous knowledge of statistics are also significant predictors.
Conclusions: The results point to increased items’ difficulty when NOTA is included, although not all the examinees seem to show the same behaviour when dealing with NOTA options.
Workflow for Phenotyping Sugar Beet Roots by Automated Evaluation of Cell Characteristics and Tissue Arrangement Using Digital Image Processing
Authors: Nause Nelia , Yamati Facundo Ramón Ispizua , Seidel Marion , Mahlein Anne-Katrin , Hoffmann Christa M. 
Published: September 2022

Abstract
Background
Cell characteristics, including cell type, size, shape, packing, cell-to-cell-adhesion, intercellular space, and cell wall thickness, influence the physical characteristics of plant tissues. Genotypic differences were found concerning damage susceptibility related to beet texture for sugar beet (Beta vulgaris). Sugar beet storage roots are characterized by heterogeneous tissue with several cambium rings surrounded by small-celled vascular tissue and big-celled sugar-storing parenchyma between the rings. This study presents a procedure for phenotyping heterogeneous tissues like beetroots by imaging.
Results
Ten Beta genotypes (nine sugar beet and one fodder beet) were included to establish a workflow for the automated histologic evaluation of cell characteristics and tissue arrangement using digital image processing written in the programming language R. The identification of cells has been validated by comparison with manual cell identification. Cells are reliably discriminated from intercellular spaces, and cells with similar morphological features are assigned to biological tissue types.
Conclusions
Genotypic differences in cell diameter and cell arrangement can straightforwardly be phenotyped by the presented workflow. The presented routine can further identify genotypic differences in cell diameter and cell arrangement during early growth stages and between sugar storage capabilities.
Dispersal and persistence traits inform long-term understory plant community change in encroached savannas
Authors: Ahler Sam J. , Ladwig Laura M. , Charton Katherine T. , Henn Jonathan J. , Damschen Ellen I. 
Published: September 2022

Abstract
Savanna plant communities are highly diverse, characterized by an open-canopy structure with a rich herbaceous understory, and maintained by frequent low-intensity fire and grazing. Due to habitat loss and fragmentation, savannas are globally threatened, with less than 1% of former oak savanna land cover found in the Midwestern United States remaining. In remnant oak savannas, loss of fire and grazing has led to woody encroachment and canopy closure over the past century with cascading consequences for the taxonomic composition of the understory community. Whether these taxonomic changes can be broadly predicted using species functional traits (morpho-physio-phenological characteristics that impact the fitness of a species) is a key question. We ask whether the impacts of woody encroachment on understory changes can be predicted from species’ abilities to persist (avoid extinction) and disperse (colonize new areas). Specifically, we pair persistence traits (e.g., clonality, belowground storage) and dispersal traits (e.g., seed mass, dispersal mode) with a rare 60 year dataset from oak savanna understories in Wisconsin, USA to understand how the representation of these traits has changed in the understory community over time. Over 60 years, savanna understory change was explained less by species persistence than dispersal abilities; small-seeded species reliant on unassisted dispersal and moderately clonal species experienced the greatest losses. These changes in functional composition are likely due to increased woody encroachment, which may impede propagule production and movement. Restoration efforts need to prioritize species that are dispersal limited and those that create fine fuels, which help maintain open fire-maintained open habitat savannas.
ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens
Authors: Fan Haobo , Wang Ying , Zou Yunchun , Song Weiqi , Xie Juan , Tang Xiuping , Chen Siyu 
Published: September 2022

Abstract
Purpose
The present study compared the expression of activity-regulated cytoskeleton-associated (ARC/Arg3.1) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of ARC/Arg3.1 in the lateral geniculate body in the pathogenesis of amblyopia.
Methods
Twenty kittens were randomly divided into experimental group (n = 10) and control group (n = 10). Black opaque covering cloth was used to cover the right eye of kittens in the experimental group. Pattern visual evoked potentials (PVEP) were detected weekly in all kittens. The expression of ARC/Arg3.1 gene was detected by immunohistochemistry and in situ hybridization, and apoptosis of lateral geniculate body cells was detected by TUNEL.
Results
PVEP detection showed that at the age of 5 and 7 weeks, the latency of P100 in the right eye of the experimental group was higher than that of the other three groups (P < 0.05), and the amplitude of P100 was lower than that of the other three groups (P < 0.05). The expression of ARC/Arg3.1 protein (P < 0.05) and mRNA (P < 0.05) in the lateral geniculate body of the experimental group was significantly lower than that of the control group. And the level of neuronal apoptosis in the experimental group was higher than that in the control group (P < 0.05). The expression of ARC/Arg3.1 gene was negatively correlated with the apoptosis level of lateral geniculate body neurons.
Conclusions
The expression of ARC/Arg3.1 is associated with monocular form deprivation amblyopia and apoptosis of lateral geniculate body cells.
The differences in the post-liver transplant outcomes of patients with autoimmune hepatitis who present with overlapping autoimmune liver diseases
Authors: Lee David Uihwan , Ponder Reid , Lee Kijung , Fan Gregory Hongyuan , Chou Harrison , Urrunaga Nathalie Helen 
Published: September 2022

Abstract
Background
Patients with autoimmune hepatitis (AIH) may co-present with diagnostic features of primary biliary cholangitis (PBC) or primary sclerosing cholangitis (PSC). Using a national transplant registry, the outcomes of patients with these presentations were compared.
Methods
The UNOS-STAR registry was used to select a study population of AIH liver transplant (LT) patients. Living and multi-organ transplant cases were excluded. Using the UNOS-registered diagnoses, the study population was subdivided into those with nonoverlapping AIH, those with AIH and PBC (AIH-PBC), and those with AIH and PSC (AIH-PSC). Specific endpoints included all-cause mortality, graft failure, and organ-system specific causes of death.
Results
There were 2048 entries included with 1927 having nonoverlapping AIH, 52 having PSC overlap, and 69 having PBC overlap. Patients with PBC overlap were more likely to have graft failure (aHR 3.53 95% CI 1.73–1.74), mortality secondary to general respiratory causes (aHR 3.55 95% CI 1.22–10.36), mortality secondary to acute respiratory distress syndrome (ARDS) (aHR 18.07 95% CI 3.331–98.74), and recurrent disease (aHR 9.65 95% CI 1.82–51.15). Case incidence rates reflected these findings, expressed in events per 1000 person-years (For the PBC overlap and nonoverlapping AIH cases, respectively. Graft failure: 28.87 events vs. 9.15 events, mortality secondary to general respiratory causes: 12.83 deaths vs. 3.87 deaths, ARDS: 6.42 deaths vs. 0.43 deaths, recurrent disease: 6.42 deaths vs. 1.18 deaths). No increased risks were found in the cohort with PSC overlap.
Conclusion
Patients with PBC diagnostic overlap may have greater risks for respiratory-induced mortality compared to nonoverlapping AIH. Further investigations are warranted to confirm these results.
Prediction of the anisotropic effective moduli of shales based on the Mori-Tanaka model and the digital core technique
Authors: Wang Zidong , Cao Gongqi , Liu Jianlin , Fu Li-Yun 
Published: September 2022

Abstract
Natural rocks belong to the polymineral composite material with complex microstructures. Such a strong heterogeneity of rocks makes it difficult to estimate the effective moduli by traditional models in theory. In the present study, a Mori-Tanaka (MT) model considering the shape and orientation of inclusion minerals obtained by the micro-CT is established, and then it is applied to evaluate the anisotropic parameters of shales. In the MT model, the principal radii and Eulerian angles of the ellipsoidal inclusion are obtained by solving its inertia matrix through the micro-CT. According to these inclusion information, we make statistics on the ratio of average principal radii and the distribution of Eulerian angles of inclusions with different minerals. In what follows, the effective elastic stiffness matrix of shale samples is predicted by the MT model, and the corresponding digital core is input for finite element method (FEM) analysis to verify the accuracy of the theoretical results. It is shown that the anisotropy of the elastic stiffness matrix predicted by the MT model and FEM is consistent under two sizes of representative volume elements. These findings are potential for applications in rock mechanics, civil engineering and oil exploitation, etc.
To Investigate Different Parameters of Economic Sliding Based 1 Seismic Isolation System
Authors: Bibi Tayyaba , Ali Amir , Khan Akhtar Naeem , Zhang Chunwei , Ahmad Naveed 
Published: September 2022

Abstract

Masonry structures are frequently constructed in developing countries because of their affordability and ease of construction, despite the fact that they are vulnerable and perform poorly during earthquakes. Experimental and numerical studies were carried out to determine the optimum thickness of the isolation layer for a recently developed, low-cost seismic isolation system known as the Reinforced-Cut-Wall(RCW). The parameters of the isolation layer were optimized during the experimental and numerical investigation using hollow concrete blocks. Quasi-static cyclic tests were performed on 1:3 reduced-scale brick masonry wall, incorporating the RCW isolation scheme, validating the better performance of the proposed isolation technique.
The Opportunity Driven Entrepreneurship in the Context of Innovation Systems in Europe in the Period 2010-2019
Authors: Leogrande Angelo , Costantiello Alberto , Laureti Lucio 
Published: September 2022

Abstract

In this article we have estimated the value of “Opportunity Driven Entrepreneurship” in Europe. We use data from European Innovation Scoreboard-EIS of the European Commission for 36 countries in the period 2010-2019. We use Panel Data with Fixed Effects, Panel Data with Random Effects, WLS, Pooled OLS, and Dynamic Panel. Our results show that “Opportunity Driven Entrepreneurship” is positively associated, among others, to “Innovation Friendly Environment” and “Turnover Share Large Enterprises”, while it is negatively associated, among others, to “Sales Impacts” and “R&D Expenditure Business Sectors”.

JEL Classification: O30; O31, O32; O33; O36.
A New Approach for Reducing Virtual Reality Sickness in Real Time: Design and Validation Study
Authors: Won JuHye , Kim Yoon Sang 
Published: September 2022

Background
Recently, technology that provides virtual reality (VR) content based on streaming services has been rapidly developed. However, there have been few studies to reduce VR sickness that occurs while the user watches VR content while wearing a head-mounted display (HMD) in real time.

Objective
Based on this background, we propose a new approach to measure and reduce VR sickness that occurs while the user watches VR content while wearing an HMD in real time.

Methods
The proposed approach is to apply VR sickness reduction methods in accordance with the user’s real-time VR sickness level. Three methods that are known to be effective in reducing VR sickness and a single type of VR content were used to examine the effectiveness of the proposed approach, which was confirmed by the experimental results.

Results
Our results show that VR sickness significantly decreased when a new approach was applied to VR content (in all cases, P<.05).

Conclusions
From our results, it was confirmed that VR sickness could be measured without wearing additional equipment, and its reduction method could be applied in real time in accordance with the user’s condition by the proposed approach in this paper.
ENSINO DE ESPANHOL PARA ESTUDANTES BRASILEIROS DE FORMA REMOTA E O PAPEL DAS ESTRATÉGIAS COMUNICATIVAS
Authors: Silva Gomes Aline 
Published: September 2022

Com o advento da pandemia de Covid-19, diferentes instituições no Brasil e no mundo passaram a colocar em prática, de modo mais intenso, o ensino remoto. Essa solução temporária, que tem como principal recurso a internet, vem sendo adotada para dar prosseguimento às atividades pedagógicas em diversos contextos. Visando contribuir nesta discussão, neste texto temos o objetivo de discorrer sobre o papel das estratégias de comunicação na aprendizagem de Espanhol como Língua Estrangeira (ELE) bem como destacar a importância da expressão corporal no ensino remoto. Neste trabalho, apresentamos três atividades pedagógicas desenvolvidas com o intuito de auxiliar um grupo de estudantes de espanhol a aprimorar a expressão oral no que tange à pronúncia e ao uso das estratégias de comunicação nesse idioma. Para elaborar as tarefas, utilizamos dois aplicativos: Whatsapp e Anchor. Como resultado, observamos uma mudança de comportamento da turma no ambiente de sala de aula virtual; os alunos mais reservados passaram a abrir a câmera e o microfone e as participações se tornaram mais ativas e espontâneas.
Effect of SARS-CoV-2 Infection and Infection Severity on Longer-Term Glycemic Control and Weight in People With Type 2 Diabetes
Authors: Wong Rachel , Vaddavalli Rohith , Hall Margaret A. , Patel Monil V. , Bramante Carolyn T. , Casarighi Elena , Johnson Steven G. , Lingam Veena , Miller Joshua D. , Reusch Jane , Saltz Mary , Stürmer Til , Tronieri Jena S. , Wilkins Kenneth J. , Buse John B. , Saltz Joel , Huling Jared D. , Moffitt Richard 
Published: September 2022

<p> </p>
<p><em>Objective: </em>To evaluate the association between COVID-19 infection and severity of infection on longer-term glycemic control and weight in persons with type 2 diabetes mellitus (T2D) in the U.S.</p>
<p><em>Research Design and Methods:</em> We conducted a retrospective cohort study using longitudinal electronic health record data of patients with COVID-19 infection from the National COVID Cohort Collaborative (N3C). Patients were ≥18 years old with an ICD-10 diagnosis of T2D and at least 1 HbA1c and weight measurement prior to and after an index date of their first COVID-19 diagnosis or negative SARS-CoV-2 test. We used propensity scores to identify a matched cohort balanced on demographic characteristics, comorbidities, and medications used to treat diabetes. The primary outcome was the post-index average hemoglobin A1c (HbA1c) and post-index average weight over a 1-year time period beginning 90 days after the index date among patients who did and did not have COVID-19 infection. Secondary outcomes were post-index average HbA1c and weight in patients who required hospitalization or mechanical ventilation.</p>
<p><em>Results: </em>There was no significant difference in the post-index average HbA1c or weight in patients who had COVID-19 infection compared to controls. Mechanical ventilation was associated with a decrease in average HbA1c after COVID-19 infection.</p>
<p><em>Conclusions: </em>In a multicenter cohort of patients in the U.S. with pre-existing T2D, there was no significant change in longer-term average HbA1c or weight among patients who had COVID-19 infection. For patients with COVID-19 infection, mechanical ventilation was associated with a decrease in HbA1c after infection.</p>
Coronavirus disease 2019 vaccination-related pericarditis: a single tertiary-center experience
Authors: Collini Valentino , Imazio Massimo , De Biasio Marzia , Sinagra Gianfranco 
Published: September 2022

Aims
Vaccination represents a cornerstone of prevention in the COVID-19 pandemic. Rare adverse events including acute pericarditis and myopericarditis have been reported.

Methods
All consecutive patients referred to our referral center for pericardial diseases following COVID-19 vaccination from 1 April 2021 to 15 April 2022 were included. Acute pericarditis and myopericarditis were diagnosed according to ESC guidelines. Patients with SARS-CoV-2 infection were excluded from the study.

Results
Twenty-four patients (79% men) aged 39.7 ± 19.8 years were referred to our center with pericarditis after receiving COVID-19 vaccination. Thirteen (54%) patients were diagnosed with myopericarditis. The mean time between vaccination and symptoms onset was 7.0 ± 4.9 days, and the most frequent symptom was pericarditic chest pain (83%). Respectively, 50 and 33% of patients presented after the second and the third dose of the vaccine. Almost all patients were treated with both nonsteroidal anti-inflammatory drugs and colchicine. Five patients (21%) experienced a recurrence of pericarditis. No patient died or developed constrictive pericarditis. Mean follow-up was 8.0 ± 3.2 months.

Conclusion
COVID-19 vaccine-related pericarditis typically manifest with mild clinical signs, in young male individuals, a few days after the second or third vaccine dose and are commonly characterized by a rapid complete recovery.
A 20-year experience in cardiac tumors: a single center surgical experience and a review of literature
Authors: Restivo Luca , De Luca Antonio , Fabris Enrico , Pagura Linda , Pierri Alessandro , Korcova Renata , Franzese Ilaria , Fiocco Alessandro , Rauber Elisabetta , Mazzaro Enzo , Bussani Rossana , Belgrano Manuel , Pappalardo Aniello , Sinagra Gianfranco 
Published: September 2022

Introduction
Cardiac tumors are rare and heterogeneous entities which still remain a diagnostic and therapeutic challenge. The treatment for most cardiac tumors is prompt surgical resection. We sought to provide an overview of surgical results from a series of consecutive patients treated at our tertiary care center during almost a 20-year experience.

Methods and Results
In this single center study, 55 consecutive patients with diagnosis of cardiac tumor underwent surgical treatment from January 2002 to April 2021. Of these, 23 (42%) were male and the mean age was 62 ± 12 years. Fifteen (27%) patients were symptomatic at the time of the diagnosis, mostly for dyspnea and palpitations. The most frequent benign cardiac tumor was myxoma (32; 58%), occurring mainly in the left atrium (31; 97%). Pleomorphic sarcoma was the most frequent primary malignant cardiac tumor (4; 7%), mainly located in the ventricles (1; 25% in the left ventricle; 2; 50% in the right ventricle). In all cases of benign tumors surgery was successful with no relapses. Two (50%) pleomorphic sarcomas showed subsequent relapses. After a median follow-up of 44 months, 15 (27%) patients died. Although malignant tumors presented a limited survival, benign tumors showed a very good prognosis.

Conclusion
Cardiac tumors require a multidisciplinary approach to guarantee a prompt diagnosis and appropriate treatment. In our surgical experience, outcome after surgery of benign tumors was excellent, while malignant tumors had poor prognosis despite radical surgery.