bluuebunny/crossref_metadata_2025_split huggingface dataset:
The interactive work of narrative elicitation in person‐centred care: Analysis of phone conversations between health care professionals and patients with common mental disorders
Authors: Cederberg Matilda , Fors Andreas , Ali Lilas , Goulding Anneli , Mäkitalo Åsa 
Published: February 2022

AbstractIntroductionNarratives play a central part in person‐centred care (PCC) as a communicative means of attending to patients' experiences. The present study sets out to explore what activities are performed and what challenges participants face in the interactive process of narrative elicitation, carried through in patient‐professional communication in a remote intervention based on PCC.MethodsData were gathered from a randomized controlled trial (RCT) in a Swedish city where health care professionals (HCPs) conducted remote PCC for patients on sick leave due to common mental disorders. A sample of eleven audio‐recorded phone conversations between HCPs and patients enroled in the RCT were collected and subjected to conversation analysis.ResultsThree interactive patterns in narrative elicitation were identified: Completed narrative sequences driven by the patient, question‐driven narrative sequences guided by the HCP, and narrative sequences driven as a collaborative project between the patient and the HCP. In the question‐driven narrative sequences, communication was problematic for both participants and they did not accomplish a narrative. In the other two patterns, narratives were accomplished but through various collaborative processes.ConclusionThis study provides insight into what challenges narrative elicitation may bring in the context of a remote PCC intervention and what interactive work patients and HCP need to engage in. Importantly, it also highlights tensions in the ethics of PCC and its operationalization, if the pursuit of a narrative is not properly balanced against the respect for patients' integrity and personal preferences. Our findings also show that narrative elicitation may represent an interactive process in PCC in which illness narratives are jointly produced, negotiated and transformed.Patient or Public ContributionStakeholders, including patient representatives, were involved in the design of the main study (the RCT). They have been involved in discussions on research questions and dissemination throughout the study period. They have not been involved in conducting the present study.
The involvement matrix as a framework for involving youth with severe communication disabilities in developing health education materials
Authors: Dada Shakila , May Adele , Bastable Kirsty , Samuels Alecia , Tönsing Kerstin , Wilder Jenny , Casey Maureen , Ntuli Constance , Reddy Vasu 
Published: February 2022

AbstractIntroductionInvolving youth with severe communication disabilities in health research is foregrounded in a perspective of rights and participation. Researchers aligned with a participatory and inclusive research agenda recommend that involving youth in health research should be a deliberate and well‐planned process. However, limited examples exist of how researchers can facilitate the involvement of youth with severe communication disabilities in research projects.MethodThe aim of this paper was to describe the application of the Involvement Matrix as a conceptual framework to guide the three phases of a research project with youth with severe communication disabilities.ResultsSix youth aged 19–34 years consented to be involved in the project. All youth had a severe communication disability and used augmentative and alternative communication (AAC) to support their involvement in the research project. The Involvement Matrix provided a structure to delineate four involvement roles in three research phases: In Phase 1, youth were listeners to research information and advisors in the needs analysis. In Phase 2, as advisors and decision‐makers, youth provided their opinions on selecting picture communication symbols for health materials. In Phase 3, as partners, they were copresenters at an online youth forum.ConclusionThe Involvement Matrix was used to plan and implement the involvement of youth with severe communication disabilities in codeveloping health materials for use during the COVID‐19 pandemic. The Involvement Matrix can be applied together with AAC to enable meaningful involvement of youth in a health research project as listeners, advisors, decision‐makers and partners.Patient or Public ContributionThis study project was codeveloped with youth with severe communication disabilities who use AAC in South Africa. A person with lived experience was involved as an advisor to the health material development process and in the drafting of the manuscript.
A scoping review of clinical skill development of preregistration registered nurses in Australia and five other English‐speaking countries
Authors: Currie Jane , Thompson Cristina , Grootemaat Pam , Andersen Patrea , Finnegan Alan , Carter Michael , Halcomb Elizabeth 
Published: February 2022

AbstractAimThe aim of this scoping review is to synthesise current evidence around the clinical skill development of preregistration registered nurses (RNs) in Australia, United Kingdom (UK), Ireland, United States (US), Canada and New Zealand, to inform nurse education, policy and clinical practice.BackgroundNursing is a practical profession, and registered nurses require specific skills, knowledge and attributes in order to care for patients safely. The context for health care delivery is shifting, and the education of nurses must adapt to effectively equip the registered nurse of the future.DesignA scoping review was conducted of clinical skill development in preregistration nurses. CINAHL Plus, MEDLINE, Health Source (Nursing/Academic edition) and Scopus were searched. Included studies were primary Australian studies and international literature reviews, which focussed on preregistration nursing education. Papers were written in the English language and focussed on clinical skill development. Results were synthesised narratively. The review is reported here in accordance with the Preferred Reporting Items for Systematic Review and Meta‐analyses Scoping Review extension (PRISMA‐ScR) guidelines.ResultsOne hundred fifty‐five Australia studies and 89 international reviews were included in the review. Six key themes were identified, namely clinical skills, approaches to teaching and learning, interprofessional education, assessment of learning, clinical placement and simulation.ConclusionThere is substantial variation in strategies and programmes to facilitate clinical skill development both within Australia and internationally, indicating a genuine shift away from traditional didactic pedagogy. New graduate registered nurses were expected to be “work‐ready,” albeit at a novice level, when they enter the workplace. Future research should consider measures of impact on actual clinical practice and focus on developing work‐ready graduates for the range of clinical settings in which they may practice. Educators, policymakers and educational institutions can use these findings to inform curriculum developments to ensure that clinical skill development is evidence‐based.
A school‐based health centre partnership: Faculty practice, nursing student learning and wellness in youth, families and community
Authors: Beierwaltes Patricia , Bell Sue Ellen , Cornell Rhonda , Ostrow Laurel Gail , Schmitz Nicole , Verchota Gwen , Clisbee David , Houston Rebecca , Eggenberger Sandra K. 
Published: February 2022

AbstractAims and ObjectivesThis paper describes the development of a SBHC with an innovative model of care that grew out of a partnership between a public‐school district and a university nursing programme in the midwestern region of the United States.Background and PurposePersistent barriers to health and health care experienced by youth are well documented. School‐based health centres (SBHCs) can improve educational and health outcomes, positively impacting health equity. Academic systems are positioned to address health care needs of the school‐aged population, yet educators face challenges of accessing quality learning placements for students and faculty practice sites.MethodsA community‐based collaborative methodology guided the planning phases that were driven by priority needs identified by families and stakeholders. With the mission of “partnering with students, families, and communities in the promotion of health and wellness through engagement in practice, education, and research,” an ongoing dialogue over a two‐year period led to articulating a vision, designing a plan and implementing a nurse‐managed SBHC. The Standards for Reporting Qualitative Research (SRQR) checklist was considered in the preparation of this paper.ResultsIn three years, this SBHC has addressed and identified priority needs and served individual youth and families. The SBHC provides opportunities for the faculty to fulfil a practice requirement for certification and accreditation. Nursing students engage with youth and families in health education and health promotion while strengthening their technical and relational skills. Family nurse practitioner students gain valuable clinical experience. Faculty with expertise in family nursing guide family assessments, support family resiliency and direct therapeutic conversations with family units.ConclusionSBHCs serve youth, families, and community. This academic–practice partnership has the added benefit of providing faculty practice opportunities and nursing student experiential learning.Relevance to Clinical PracticeSHBCs provide practice opportunities that address needs in individuals, families, and communities. Partnerships should be considered at academic nursing programmes to support their needs and fulfil commitments to address health equity gaps.
Multiplexed flow cytometric approach for detection of anti-SARS-CoV-2 IgG, IgM and IgA using beads covalently coupled to the nucleocapsid protein
Authors: Zattoni I.F. , Huergo L.F. , Gerhardt E.C.M. , Nardin J.M. , dos Santos A.M.F. , Rego F.G.M. , Picheth G. , Moure V.R. , Valdameri G. 
Published: February 2022

Abstract
Flow cytometry has emerged as a promising technique for detection of SARS-CoV-2 antibodies. In this study, we developed an innovative strategy for simultaneous detection of immunoglobulin G (IgG), IgM and IgA. The SARS-CoV-2 nucleocapsid protein was covalently bound to functional beads surface applying sulpho-SMCC chemistry. BUV395 anti-IgG, BB515 anti-IgM, biotinylated anti-IgA1/IgA2 and BV421 streptavidin were used as fluorophore conjugated secondary antibodies. Serum and antibodies reaction conditions were optimized for each antibody isotype detection and a multiplexed detection assay was developed. This new cell-free assay efficiently discriminate COVID-19 negative and positive samples. The simultaneous detection of IgG, IgM and IgA showed a sensitivity of 88·5–96·2% and specificity of 100%. This novel strategy opens a new avenue for flow cytometry-based diagnosis.
Novel genetic sex markers reveal unexpected lack of, and similar susceptibility to, sex reversal in free‐living common toads in both natural and anthropogenic habitats
Authors: Nemesházi Edina , Sramkó Gábor , Laczkó Levente , Balogh Emese , Szatmári Lajos , Vili Nóra , Ujhegyi Nikolett , Üveges Bálint , Bókony Veronika 
Published: February 2022

AbstractAnthropogenic environmental changes are affecting biodiversity and microevolution worldwide. Ectothermic vertebrates are especially vulnerable because environmental changes can disrupt their sexual development and cause sex reversal, a mismatch between genetic and phenotypic sex. This can potentially lead to sex‐ratio distortion and population decline. Despite these implications, there is scarce empirical knowledge on the incidence of sex reversal in nature. Populations in anthropogenic environments may be exposed to sex‐reversing stimuli more frequently, which may lead to higher sex‐reversal rate or, alternatively, these populations may adapt to resist sex reversal. We developed PCR‐based genetic sex markers for the common toad (Bufo bufo) to assess the prevalence of sex reversal in wild populations living in natural, agricultural and urban habitats, and the susceptibility of the same populations to two ubiquitous oestrogenic pollutants in a common garden experiment. We found negligible sex‐reversal frequency in free‐living adults despite the presence of various endocrine‐disrupting pollutants in their breeding ponds. Individuals from different habitat types showed similar susceptibility to sex reversal in the laboratory: all genetic males developed female phenotype when exposed to 1 µg L−1 17α‐ethinylestradiol (EE2) during larval development, whereas no sex reversal occurred in response to 1 ng L−1 EE2 and a glyphosate‐based herbicide with 3 µg L−1 or 3 mg L−1 glyphosate. The latter results do not support that populations in anthropogenic habitats would have either increased propensity for or higher tolerance to chemically induced sex reversal. Thus, the extremely low sex‐reversal frequency in wild toads compared to other ectothermic vertebrates studied before might indicate idiosyncratic, potentially species‐specific resistance to sex reversal.
Seascape genomics of coastal bottlenose dolphins along strong gradients of temperature and salinity
Authors: Pratt Eleanor A. L. , Beheregaray Luciano B. , Bilgmann Kerstin , Zanardo Nikki , Diaz‐Aguirre Fernando , Brauer Chris , Sandoval‐Castillo Jonathan , Möller Luciana M. 
Published: February 2022

AbstractHeterogeneous seascapes and strong environmental gradients in coastal waters are expected to influence adaptive divergence, particularly in species with large population sizes where selection is expected to be highly efficient. However, these influences might also extend to species characterized by strong social structure, natal philopatry and small home ranges. We implemented a seascape genomic study to test this hypothesis in Indo‐Pacific bottlenose dolphins (Tursiops aduncus) distributed along the environmentally heterogeneous coast of southern Australia. The data sets included oceanographic and environmental variables thought to be good predictors of local adaptation in dolphins and 8081 filtered single nucleotide polymorphisms (SNPs) genotyped for individuals sampled from seven different bioregions. From a neutral perspective, population structure and connectivity of the dolphins were generally influenced by habitat type and social structuring. Genotype‐environment association analysis identified 241 candidate adaptive loci and revealed that sea surface temperature and salinity gradients influenced adaptive divergence in these animals at both large‐ (1000 km) and fine‐scales (<100 km). Enrichment analysis and annotation of candidate genes revealed functions related to sodium‐activated ion transport, kidney development, adipogenesis and thermogenesis. The findings of spatial adaptive divergence and inferences of putative physiological adaptations challenge previous suggestions that marine megafauna is most likely to be affected by environmental and climatic changes via indirect, trophic effects. Our work contributes to conservation management of coastal bottlenose dolphins subjected to anthropogenic disturbance and to efforts of clarifying how seascape heterogeneity influences adaptive diversity and evolution in small cetaceans.
Jasmonic acid coordinates with light, glucose and auxin signalling in regulating branching angle of Arabidopsis lateral roots
Authors: Sharma Manvi , Sharma Mohan , Jamsheer K Muhammed , Laxmi Ashverya 
Published: February 2022

AbstractThe role of jasmonates (JAs) in primary root growth and development and in plant response to external stimuli is already known. However, its role in lateral root (LR) development remains to be explored. Our work identified methyl jasmonate (MeJA) as a key phytohormone in determining the branching angle of Arabidopsis LRs. MeJA inclines the LRs to a more vertical orientation, which was dependent on the canonical JAR1‐COI1‐MYC2,3,4 signalling. Our work also highlights the dual roles of light in governing LR angle. Light signalling enhances JA biosynthesis, leading to erect root architecture; whereas, glucose (Glc) induces wider branching angles. Combining physiological and molecular assays, we revealed that Glc antagonises the MeJA response via TARGET OF RAPAMYCIN (TOR) signalling. Moreover, physiological assays using auxin mutants, MYC2‐mediated transcriptional activation of LAZY2, LAZY4 and auxin biosynthetic gene CYP79B2, and asymmetric distribution of DR5::GFP and PIN2::GFP pinpointed the role of an intact auxin machinery required by MeJA for vertical growth of LRs. We also demonstrated that light perception and signalling are indispensable for inducing vertical angles by MeJA. Thus, our investigation highlights antagonism between light and Glc signalling and how they interact with JA‐auxin signals to optimise the branching angle of LRs.
Auto‐inflammation and auto‐immunity pathways are associated with emergence of BOS in pediatric lung transplantation
Authors: Conrad Carol K. , Hedlin Haley , Chin Hyunsook , Hayes Don , Heeger Peter S. , Faro Albert , Goldfarb Samuel , Melicoff‐Portillo Ernestina , Thalachallour Mohanakumar , Odim Jonah , Schecter Marc , Storch Gregory A. , Visner Gary A. , Williams Nikki M. , Kesler Karen , Danziger‐Isakov Lara , Sweet Stuart C. 
Published: February 2022

AbstractBackgroundLong‐term survival after lung transplantation (LTx) is limited by chronic lung allograft dysfunction (CLAD).MethodsWe report an analysis of cytokine profiles in bronchoalveolar lavage samples collected during a prospective multicenter non‐interventional trial primarily designed to determine the impact of community‐acquired respiratory viral infections (CARV) in outcomes after pediatric LTx. In this analysis, we identify potential biomarkers of auto‐inflammation and auto‐immunity associated with survival and risk of bronchiolitis obliterans (BOS) after LTx with cytokine analysis of bronchoalveolar lavage fluid (BALF) from 61 pediatric recipients.ResultsHigher IL‐23 (p = .048) and IL‐31 (p = .035) levels were associated with the risk of BOS, and lower levels of epithelial growth factor (EGF) (p = .041) and eotaxin (EOX) (p = .017) were associated with BOS. Analysis using conditional inference trees to evaluate cytokines at each visit associated with survival identified soluble CD30 (p < .001), pro‐inflammatory cytokine IL‐23 (p = .02), and sTNFRI (p = .01) below cutoff levels as associated with BOS‐free survival.ConclusionsOur results indicate that post‐LTx survival in children may be linked to activation of alternate pathways of the immune system that affect airway remodeling in addition to activation of “classical” pathways that have been described in adult LTx recipients. These may indicate pathways to target for intervention.
Pathogenesis of follicular thymic hyperplasia associated with rheumatoid arthritis
Authors: Ohe Rintaro , Yang Suran , Yamashita Daisuke , Ichikawa Chihiro , Saito Akihisa , Kabasawa Takanobu , Utsunomiya Aya , Aung Naing Ye , Urano Yuka , Kitaoka Takumi , Suzuki Kazushi , Takahara Daiichiro , Sasaki Akiko , Takakubo Yuya , Takagi Michiaki , Yamakawa Mitsunori , Futakuchi Mitsuru 
Published: February 2022

AbstractLymphoproliferative disorders may occur in patients with rheumatoid arthritis (RA) who are treated with methotrexate. However, follicular thymic hyperplasia (FTH) associated with RA (FTH‐RA) is generally not considered a lymphoproliferative disorder. To investigate the pathogenesis of FTH‐RA, we examined 12 cases of FTH involving thymic enlargement, four of FTH involving RA and eight of FTH involving myasthenia gravis (MG). Increased numbers and larger germinal center (GC) size were observed in FTH‐RA group. The percentage of distorted GCs was 13.3% in FTH‐RA group and 3.25% in FTH associated with MG (FTH‐MG) group. A greater meshwork of follicular dendritic cells was observed in the GCs of FTH‐RA group. Positive indices of CD27+ cells and PD‐1+ cells per GC in FTH‐RA group were significantly higher than those in FTH‐MG group, though positive indices of CD68+ cells and CD163+ cells were similar. Myoid cell proliferation, as evaluated by α‐SMA, tenascin‐C, and l‐caldesmon expression, was significantly increased in the FTH‐RA group compared with the FTH‐MG group. These results suggest that FTH should be considered in patients with RA treated with methotrexate. The pathogenesis of FTH‐RA includes GC expansion and increased numbers of memory B cells, follicular helper T cells, and myoid cells, indicating humoral immunity activation.
Deep incorporation of corn straw benefits soil organic carbon and microbial community composition in a black soil of Northeast China
Authors: Zhu Fangni , Lin Xinxin , Guan Song , Dou Sen 
Published: February 2022

Abstract‘Deep incorporation of corn straw’ (CSDI) is to concentrate the burial of corn straw into the subsurface soil layer (20–40 cm) and to break the plough pan, thereby creating a loosened plough layer (0–20 cm) and a fertile subsurface soil layer. However, its impacts on soil organic carbon (SOC) and the microbial community remain poorly understood. A field experiment was conducted to investigate the effects of 1‐year CSDI (CD1), 3‐year CSDI (CD3) and 5‐year CSDI (CD5) on soil aggregates and aggregate‐associated SOC, as well as bacterial and fungal community characteristics (examined by the high‐throughput gene sequencing method). The results demonstrated that SOC and soil fungal diversity were decreased by CD1, but increased by CD3 and CD5. Compared with the control, CD5 promoted 2–0.25 mm soil macroaggregation, significantly increased SOC by 8.94% and aggregate‐associated SOC by 5.96%–8.84%, consequently improving the physical protection of SOC by soil aggregates. CD3 and CD5 enhanced the richness and diversity of soil bacteria and fungi, and altered community composition. For soil bacteria, the relative abundance of Acidobacteria and Chloroflexi was increased, while that of Firmicutes, Gemmatimonadetes, Sphingomonas and Bacillus was decreased. For soil fungi, the relative abundance of Ascomycota, Zygomycota, Mortierella and Fusarium was greatly improved, but that of Basidiomycota was reduced. These obvious variations in microbial community structure were beneficial to straw degradation and SOC accumulation. Overall, the optimization of microbial community with CSDI plays a positive role in promoting soil organic matter, nutrient cycling and carbon sequestration, and thus improving soil fertility.