bluuebunny/crossref_metadata_2025_split huggingface dataset:
SlWRKY45 interacts with jasmonate-ZIM domain proteins to negatively regulate defense against the root-knot nematodeMeloidogyne incognitain tomato
Authors: Huang Huang , Zhao Wenchao , Qiao Hui , Li Chonghua , Sun Lulu , Yang Rui , Ma Xuechun , Ma Jilin , Song Susheng , Wang Shaohui 
Published: September 2022

AbstractParasitic root-knot nematodes (RKNs) cause a severe reduction in crop yield and seriously threaten agricultural production. The phytohormones jasmonates (JAs) are important signals regulating resistance to multiple biotic and abiotic stresses. However, the molecular mechanism for JAs-regulated defense against RKNs in tomato remains largely unclear. In this study, we found that the transcription factor SlWRKY45 interacted with most JA-ZIM domain family proteins (JAZs), key repressors of the JA signaling. After infection by the RKN Meloidogyne incognita, the slwrky45 mutants exhibited lower gall numbers and egg numbers per gram of roots than wild type, whereas overexpression of SlWRKY45 attenuated resistance to Meloidogyne incognita. Under M. incognita infection, the contents of jasmonic acid (JA) and JA-isoleucine (JA-Ile) in roots were repressed by SlWRKY45-overexpression. Furthermore, SlWRKY45 bound to and inhibited the promoter of the JA biosynthesis gene ALLENE OXIDE CYCLASE (AOC), and repressed its expression. Overall, our findings revealed that the SlJAZ-interaction protein SlWRKY45 attenuated RKN-regulated JA biosynthesis and repressed defense against the RKN M. incognita in tomato.
The evolving personal, professional and physical impact on healthcare professionals during three COVID-19 waves: A cross-sectional study
Authors: Seys Deborah , Peeters Bart , Doggen Kris , Vanhaecht Kris 
Published: September 2022

Abstract

Background
The COVID-19 pandemic has led to huge pressure on not only healthcare systems, but also on healthcare professionals.

Objective
As the pandemic continues, the aim of this study is to evaluate how 10 reactions of healthcare professionals evolved during the first 18 months of COVID-19.

Methods
A repeated cross-sectional study was performed with eight measurement points between April 2020 and September 2021 in Belgium. Participants were asked how frequently (on a scale of 0–10) they experienced positive and negative reactions during normal circumstances and during past week, referred to as before and during COVID-19, respectively. These reactions were stress, fatigue, difficulty sleeping, muscle strain, hypervigilance, leaving profession, headache, doubting knowledge and skills, flashbacks and fear.

Results
In total, 13 308 respondents were included in our study. During both the first (March 2020) and second COVID-19 peak (November 2020), the measured personal, professional and physical reactions were significantly higher compared to before COVID-19. The third wave in April 2021 was shorter and less severe with regard to hospital admissions and deaths, yet an important impact on healthcare professionals could still be observed. ‘Fatigue,’ ‘stress,’ ‘difficulty sleeping’ and ‘muscle strain’ are the most worrying reactions in September 2021, which are increasing compared to the previous measurements.

Conclusion
Our results showed that acute stress reactions decreased over time but that chronic stress reactions and professional reactions, such as ‘intent to leave,’ increased. Healthcare organizations and policy makers should realize that 18 months after the start of COVID-19 almost all of the measured reactions continue to be more prevalent than before COVID-19. Moreover, the continuous increase over the last three measurement periods of the number of healthcare professionals who want to leave their profession is alarming. Continuous follow-up of the personal, professional and physical reactions is more than necessary.
Legal, Mythic, and Divine Violence: Post-secular Entanglements in Walter Benjamin’s ‘Toward the Critique of Violence’
Authors: Holte Stine 
Published: September 2022

Abstract
Walter Benjamin’s essay ‘Toward the Critique of Violence’ (1921) has in the past decades been central to important debates within post-secular philosophical thought. This article explores the intricate connections between legal, mythic, and divine violence in Benjamin’s essay, in light of some of his other theological, literary, and political works from the same period. It suggests that the idea of a ‘divine law-annihilating violence’ should be read in light of Benjamin’s claim that a ‘critique of violence is the philosophy of its history’. This implies amongst other things a critique of the dogma of the sanctity of mere life underlying both the Greek and the Christian tradition.
Performing ‘Art for the People’: Politics, Spectatorship, and Schoenberg’s Music at the Berlin VolksbÜhne
Authors: Carrasco Clare 
Published: September 2022

ABSTRACT
In early 1920, a performance of Arnold Schoenberg’s Chamber Symphony sponsored by the Berlin Volksbühne (People’s Theatre) set off a dialogue of remarkable vehemence and duration in the musical press. Writing as spectators of what they imagined to be a working-class audience, professional music critics framed the event as a musical performance that itself was a carefully staged piece of political theatre. Their accounts detail and debate the use of casting, symbols, and rituals to enact a ‘leftist’ agenda in which Schoenberg’s music was not the subversive project of a radical few, but a legitimate exemplar of ‘art for the people’—the motto of the Volksbühne. By engaging with the recent ‘performative turn’ in studies of the Weimar Republic, this article presents a rich microhistory of this experimental concert and its afterlife in the press, revealing vital connections to broader practices of political performativity in Weimar democracy.
Multiple locations of near-infrared coronal lines in NGC 5548
Authors: Kynoch Daniel , Landt Hermine , Dehghanian Maryam , Ward Martin J , Ferland Gary J 
Published: September 2022

ABSTRACT
We present the first intensive study of the variability of the near-infrared coronal lines in an active galactic nucleus (AGN). We use data from a 1-yr-long spectroscopic monitoring campaign with roughly weekly cadence on NGC 5548 to study the variability in both emission line fluxes and profile shapes. We find that in common with many AGN coronal lines, those studied here are both broader than the low-ionizaton forbidden lines and blueshifted relative to them, with a stratification that implies an origin in an outflow interior to the standard narrow line region. We observe for the first time [S viii] and [Si vi] coronal line profiles that exhibit broad wings in addition to narrow cores, features not seen in either [S ix] or [Si x]. These wings are highly variable, whereas the cores show negligible changes. The differences in both the profile shapes and variability properties of the different line components indicate that there are at least two coronal line regions in AGN. We associate the variable, broad wings with the base of an X-ray heated wind evaporated from the inner edge of the dusty torus. The coronal line cores may be formed at several locations interior to the narrow line region: either along this accelerating, clumpy wind or in the much more compact outflow identified with the obscurer and so emerging on scales similar to the outer accretion disc and broad-line region.
Detecting and analysing the topology of the cosmic web with spatial clustering algorithms I: methods
Authors: Kelesis Dimitrios , Basilakos Spyros , Papadopoulou Lesta Vicky , Fotakis Dimitris , Efstathiou Andreas 
Published: September 2022

ABSTRACT
In this paper, we explore the use of spatial clustering algorithms as a new computational approach for modelling the cosmic web. We demonstrate that such algorithms are efficient in terms of computing time needed. We explore three distinct spatial methods which we suitably adjust for (i) detecting the topology of the cosmic web and (ii) categorizing various cosmic structures as voids, walls, clusters, and superclusters based on a variety of topological and physical criteria such as the physical distance between objects, their masses, and local densities. The methods explored are (1) a new spatial method called Gravity Lattice; (2) a modified version of another spatial clustering algorithm, the abacus; and (3) the well known spatial clustering algorithm hdbscan. We utilize hdbscan in order to detect cosmic structures and categorize them using their overdensity. We demonstrate that the abacus method can be combined with the classic dtfe method to obtain similar results in terms of the achieved accuracy with about an order of magnitude less computation time. To further solidify our claims, we draw insights from the computer science domain and compare the quality of the results with and without the application of our method. Finally, we further extend our experiments and verify their effectiveness by showing their ability to scale well with different cosmic web structures that formed at different redshifts.
Finite-temperature effects in dynamical spacetime binary neutron star merger simulations: validation of the parametric approach
Authors: Raithel Carolyn A , Espino Pedro , Paschalidis Vasileios 
Published: September 2022

ABSTRACT
Parametric equations of state (EoSs) provide an important tool for systematically studying EoS effects in neutron star merger simulations. In this work, we perform a numerical validation of the M*-framework for parametrically calculating finite-temperature EoS tables. The framework, introduced by Raithel et al., provides a model for generically extending any cold, β-equilibrium EoS to finite temperatures and arbitrary electron fractions. In this work, we perform numerical evolutions of a binary neutron star merger with the SFHo finite-temperature EoS, as well as with the M*-approximation of this same EoS, where the approximation uses the zero-temperature, β-equilibrium slice of SFHo and replaces the finite-temperature and composition-dependent parts with the M*-model. We find that the approximate version of the EoS is able to accurately recreate the temperature and thermal pressure profiles of the binary neutron star remnant, when compared to the results found using the full version of SFHo. We additionally find that the merger dynamics and gravitational wave signals agree well between both cases, with differences of $\lesssim 1\!-\!2\,{\textrm{per cent}}$ introduced into the post-merger gravitational wave peak frequencies by the approximations of the EoS. We conclude that the M*-framework can be reliably used to probe neutron star merger properties in numerical simulations.
Rotavirus RNA chaperone mediates global transcriptome-wide increase in RNA backbone flexibility
Authors: Coria Aaztli , Wienecke Anastacia , Knight Michael L , Desirò Daniel , Laederach Alain , Borodavka Alexander 
Published: September 2022

Abstract
Due to genome segmentation, rotaviruses must co-package eleven distinct genomic RNAs. The packaging is mediated by virus-encoded RNA chaperones, such as the rotavirus NSP2 protein. While the activities of distinct RNA chaperones are well studied on smaller RNAs, little is known about their global effect on the entire viral transcriptome. Here, we used Selective 2′-hydroxyl Acylation Analyzed by Primer Extension and Mutational Profiling (SHAPE-MaP) to examine the secondary structure of the rotavirus transcriptome in the presence of increasing amounts of NSP2. SHAPE-MaP data reveals that despite the well-documented helix-unwinding activity of NSP2 in vitro, its incubation with cognate rotavirus transcripts does not induce a significant change in the SHAPE reactivities. However, a quantitative analysis of mutation rates measured by mutational profiling reveals a global 5-fold rate increase in the presence of NSP2. We demonstrate that the normalization procedure used in deriving SHAPE reactivities from mutation rates can mask an important global effect of an RNA chaperone. Analysis of the mutation rates reveals a larger effect on stems rather than loops. Together, these data provide the first experimentally derived secondary structure model of the rotavirus transcriptome and reveal that NSP2 acts by globally increasing RNA backbone flexibility in a concentration-dependent manner.
KS05.7.A Characterization of the immune composition of extreme long-term survivors with malignant glioma at single-cell level
Authors: Decraene B , Antoranz A , Verbeke T , Nazari P , Solie L , Dubroja N , Derweduwe M , De Smet F , De Vleeschouwer S 
Published: September 2022

Abstract

Background
Glioblastoma Multiforme (GBM) remains the most common malignant primary brain tumor with a dismal prognosis that rarely exceeds beyond two years despite extensive therapy, which consists of maximal safe surgical resection, radiotherapy and/or chemotherapy. Recently, it has become clear that GBM is not one homogeneous entity and that both intra-and intertumoral heterogeneity contribute significantly to differences in tumoral behavior which may consequently be responsible for differences in survival. Strikingly and in spite of its dismal prognosis, small fractions of GBM patients seem to display extended survival compared to the large majority of patients. The underlying mechanisms for this peculiarity remain largely unknown however, even though emerging data suggest that both cancer cell-autonomous and microenvironmental factors and their interplay probably play an important role.

Material and Methods
We used high-dimensional, multiplexed immunohistochemistry to spatially, and cytometry by time-of-flight to quantitively characterize the cell constitution and interactions within the tumor microenvironment (TME) in 21 extreme long-term survivors (living over ten years since primary diagnosis or five years after recurrence) and 42 deeply matched short-term controls (living under 1.5 year) on a single cell level. For all tumors (epi-)genetic data was also collected.

Results
We identified a high level of both inter-and intrapatient heterogeneity defined by several distinct tumor niches, as well as described interactions within these niches and with the surrounding infiltrating immune cells of the TME. By linking patient characteristics with the heterogeneous immune composition we are building an immune stratification that can be linked to patient survival in GBM.

Conclusion
Generating an immune stratification for GBM will allow us to identify immune characteristics responsible for longer or even exceptional survival, as well as thoroughly identify tumor components that may serve as a potential target for personalized treatment strategies. Therefore, this study is also an essential initial step towards such clinical trials which alter the TME in a favorable way with a personalized modulation strategy.
OS06.5.A Investigating safety and efficacy of TTFields prior and concomitant to radiotherapy in newly diagnosed glioblastoma - the PriCoTTF phase I/II trial
Authors: Kebir S , Lazaridis L , Schmidt T , Oster C , Feldheim J , Glas M , Pierscianek D , Proescholdt M , Hau P , Grosu A , Krex D , Scheffler B , Kleinschnitz C , Pöttgen C , Stuschke M 
Published: September 2022

Abstract

Background
The EF-14 phase III trial demonstrated improved survival for patients with newly diagnosed glioblastoma (nGBM) when TTFields therapy was added to adjuvant temozolomide chemotherapy. TTFields at 200 kHz are applied to the tumor utilizing arrays on the patients’ scalp. In preclinical studies, a synergistic inhibiting effect on glioblastoma cell proliferation was found for the combination of TTFields and radiotherapy. Based on these findings, we conduct the phase I/II PriCoTTF trial in adult nGBM patients to investigate the safety and efficacy of TTFields therapy initiated prior and concomitant to radiochemotherapy.

Material and Methods
Per study protocol, TTFields therapy is initiated following surgery and completed wound healing. Continuing throughout radiochemotherapy and adjuvant chemotherapy, TTFields therapy is used for approximately 9 months in total with TTFields rechallenge allowed at recurrence. Radiotherapy is conducted with arrays applied on the patients’ scalp. A total recruitment of 33 patients was sought, with 20 patients in arm A receiving normo-fractionated radiotherapy, and 13 elderly patients in arm B receiving hypo-fractionated radiotherapy. Safety and tolerance are the study’s primary endpoint, analyzed by a selection of pre-specified treatment-limiting toxicities (TLTs).

Results
A total of 33 patients have been enrolled. Patients' characteristics were mostly typical for glioblastoma, except for a rather low fraction of patients with gross total resection (GTR, 22.5%). The distribution of adverse events of common toxicity criteria (CTC) grade 3 or higher was comparable to that of established glioblastoma trials. Notably, skin toxicity of CTC grade 3 or higher was quite uncommon (n=2, 6%). As no patient developed TLTs, the study's primary endpoint was met. Median TTFields treatment duration was 8.4 months. Overall survival data was not mature enough (event rate 48%) to allow for a definite conclusion. Notably, on multivariable Cox regression, the number of days with TTFields adherence of more than 23 hours was independently associated with overall survival (HR 0.96, 95% confidence interval 0.93 - 0.99, p=0.008).

Conclusion
The PriCoTTF trial met its primary endpoint indicating that combined TTFields and radiotherapy is safe and well tolerated. High-grade skin toxicity was quite uncommon and the patients with high TTFields adherence seem to perform particularly well. An extended follow-up is required to provide first estimates regarding putative efficacy. At that point in time, the reduced overall TTFields duration and fraction of patients with GTR need to be factored in.
OS08.6.A Novel role of ARF4-mediated retrograde trafficking as a driver of chemoresistance in GBM
Authors: Budhiraja S , Baisiwala S , Perrault E , Cho S , Dara G , Nandoliya K , Zolp A , Chen L , Dmello C , Park C , Sonabend A , Ahmed A 
Published: September 2022

Abstract

Background
Glioblastoma (GBM) is the most common type of adult malignant brain tumor, with a median survival of only 21 months. This is partly due to the high rate of resistance to conventional therapy, including temozolomide (TMZ), leading to recurrence rates close to 100%. To identify the unknown genes driving the development of this resistance, we performed a genome-wide CRISPR knockout screen comparing a DMSO-treated population with a TMZ-treated population over 14 days. Results showed substantial enrichment of ~200 novel genes, including a previously unstudied gene ARF4—involved in retrograde trafficking to the nucleus. Here, we set out to characterize the mechanism by which ARF4 may be acting to promote chemoresistance.

Material and Methods
A whole-genome CRISPR-Cas9 sensitivity screen as well as a variety of in vitro and in vivo experiments, such as live-cell imaging, cell viability assays, and western blotting were conducted.

Results
Initial investigation into ARF4 showed significant elevations in expression at RNA and protein levels (p&lt;0.05) in recurrent patient tumors, as well as a significant survival benefit in patient datasets when downregulated (p&lt;0.05). Knocking out ARF4 resulted in significantly heightened sensitivity to TMZ in multiple GBM patient-derived xenograft lines and extended survival compared to the controls (p&lt;0.01) in vivo. Further investigation via live-cell imaging of transferrin receptors, a retrograde transport marker, revealed that ARF4-knockdowns significantly inhibited retrograde trafficking, while ARF4-overexpressions resulted in an untenable increase in retrograde trafficking in vitro. This effect was also seen in TMZ-treated cells, which displayed enhanced trafficking dynamics, suggesting that ARF4-mediated retrograde trafficking is elevated during therapy to drive nuclear localization of key chemoresistance-promoting factors. We then performed an unbiased proteomics screen to identify which genes were being uniquely transported to the nucleus as a product of ARF4-mediated retrograde trafficking, which revealed enrichment of the EGFR signaling pathway in particular. Validation experiments confirmed a decrease in EGFR trafficking and nuclear EGFR expression in ARF4-knockdowns and an increase in EGFR trafficking and nuclear EGFR expression in ARF4-overexpression and TMZ-treated GBM cells. Furthermore, nuclear DNA-PK, a DNA repair protein known to be transcriptionally activated by EGFR, was similarly found to be downregulated in ARF4-knockdown conditions and elevated in ARF4-overexpression and TMZ-treated conditions.
Conclusion: Here, we show that ARF4 may be responsible for promoting chemoresistance through altered retrograde trafficking of EGFR specifically. Thus, our study has yielded a promising and novel therapeutic target for GBM, a disease desperately in need of new therapeutic strategies.
P01.08.A Perioperative Levetiracetam for seizure prophylaxis in seizure naive brain tumor patients with focus on neurocognitive functioning
Authors: Konrath E , Marhold F , Kindler W , Scheichel F , Popadic B , Blauensteiner K , Calabek-Wohinz B , Freydl E , Weber M , Ristl R , Hainz K , Sherif C , Oberndorfer S 
Published: September 2022

Abstract

Background
In seizure-naive brain tumor patients, the efficacy of perioperative prophylactic antiepileptic drugtreatment remains controversial. In case of administration, the common preferred drug is levetiracetam (LEV) because of its favorable pharmacological profile. Research to date has not sufficiently determined how LEV affects cognition in the short term as it does in the perioperative period. The objective of this prospective study was to examine the neurocognitive functioning of seizure-naive brain tumor patients after receiving LEV perioperatively.

Material and methods
Patients with supratentorial brain tumor undergoing surgery received LEV three days before until seven days after surgery as seizure prophylaxis. Cognitive functioning (NeuroCogFX), LEV plasma-levels, hematotoxicity, side-effects, as well as health-related quality of life (HRQoL, Qolie31), were recorded preoperatively before (Baseline) and after onset of LEV (Pre-Op), 4-6 days postoperatively (Post-Op) and 21 days postoperatively (Follow-Up).

Results
No significant changes in cognitive functioning and HRQoL were seen after onset of preoperative LEV. There was a significant improvement of NeuroCogFX total-score at Follow-Up (p=0.004) compared to Baseline. The overall-score Qolie31 showed simultaneous improvement patterns as cognitive functioning (p&lt; 0.001). The most frequent side effect related to study drug was somnolence (in 24% of patients).

Conclusion
Following LEV therapy, a significant improvement of cognitive functioning, as well as an improvement in HRQoL, were detected postoperatively. This is presumably due to the debulking effect of the surgery. Nevertheless, LEV has no detrimental effect on cognitivefunctioning in the perioperative phase in seizure-naive brain tumor patients.