bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulating the Electronic Structure of RuO2 through Cr Solubilizing for Improved Oxygen Evolution Reaction
Authors: Chen Haixin , Zhang Xiaofeng , Geng Shipeng , Song Shuqin , Wang Yi 
Published: July 2022

AbstractHydrogen production from water electrolysis is important for the sustainable development of hydrogen energy. Nevertheless, the naturally torpid property of anodic oxygen evolution reaction (OER) kinetics and poor stability of its catalysts significantly restrict the development of electrochemical water splitting. Here, a Ru0.6Cr0.4O2 electrocatalyst is synthesized, which reveals excellent OER activity with the overpotential of only 195 mV at 10 mA cm−2 and excellent stability with the potential increase of merely 5.3 mV after 20 h continuous OER test in acidic media. Theoretical calculations reveal that the solubilizing of Cr into RuO2 could adjust the electron distribution, making the d‐band center of Ru far away from the Fermi level. This behavior reduces the binding energy with Ru and O and accelerates the rate‐determining step of OER (i.e., the formation of *OOH), thereby increasing OER activity. In addition, the incorporation of Cr increases the energy of oxygen defect formation and reduces the participation of lattice oxygen, thus improving the stability of the catalyst. This research furnishes a feasible policy for the development of highly active and stable catalysts in acidic media by regulating the electronic structure of RuO2.
Exploring UK veterinary students’ use of online resources as a tool for studying small animal internal medicine
Authors: Bevis Ashleigh J. R. , Saadeh Khalil , Tse Gary , Jeevaratnam Kamalan 
Published: July 2022

AbstractBackgroundDespite an increase in the use of online resources, their use by veterinary students, especially in the study of small animal internal medicine (SAIM), remains poorly understood.MethodsA questionnaire‐based study consisting of 26 survey items and 263 responses from seven UK universities investigated the use of online resources among clinical veterinary students studying SAIM and whether this was affected by age, gender, year of study or entry status.ResultsRandom internet searches were the preferred method of clarifying queries, except for graduate‐entry students and age categories 18–21 years and 28 years and over who preferred traditional textbooks. Online searches were preferred over emailing instructors or contacting instructors in person. Despite 73.3% of participants not automatically trusting information from online sources, only 47.1% fact‐checked, although this was higher among the graduate‐entry group (62.5%). Frequent social media use was reported; however, only 44.8% of students reported using social media to discuss SAIM. Video clip usage was high, and 82.9% of students reported video clips were useful for understanding SAIM concepts.ConclusionOnline resources are a useful tool to complement traditional resources; however, a level of academic oversight may be required to ensure appropriate and effective use of these resources.
Constraining new physics with Borexino Phase-II spectral data
Authors: Coloma Pilar , Gonzalez-Garcia M. C. , Maltoni Michele , Pinheiro João Paulo , Urrea Salvador 
Published: July 2022

AbstractWe present a detailed analysis of the spectral data of Borexino Phase II, with the aim of exploiting its full potential to constrain scenarios beyond the Standard Model. In particular, we quantify the constraints imposed on neutrino magnetic moments, neutrino non-standard interactions, and several simplified models with light scalar, pseudoscalar or vector mediators. Our analysis shows perfect agreement with those performed by the collaboration on neutrino magnetic moments and neutrino non-standard interactions in the same restricted cases and expands beyond those, stressing the interplay between flavour oscillations and flavour non-diagonal interaction effects for the correct evaluation of the event rates. For simplified models with light mediators we show the power of the spectral data to obtain robust limits beyond those previously estimated in the literature.
Surface Ornamentation Techniques and Spatial Distortion
Authors: Martin Javier , Martín Fuentes Daniel Vicente 
Published: July 2022

AbstractSeveral different types of surface-related ornamentation that are capable of distorting the perception of space have been developed and used since ancient times. Some examples of these are di sotto in sù, quadratura, trompe l’oeil and foreshortening. These techniques throw into question the limits of space and architecture and our understanding thereof. However, until now, there has been no clear classification or designation of these distortive techniques. Therefore, this article focuses on the identification and classification of three different ornamental techniques applied to surfaces that can distort the perception of space and looks to the work of various contemporary artists to exemplify the proposed taxonomy.
A Converse to Lieb–Robinson Bounds in One Dimension Using Index Theory
Authors: Ranard Daniel , Walter Michael , Witteveen Freek 
Published: July 2022

AbstractUnitary dynamics with a strict causal cone (or “light cone”) have been studied extensively, under the name of quantum cellular automata (QCAs). In particular, QCAs in one dimension have been completely classified by an index theory. Physical systems often exhibit only approximate causal cones; Hamiltonian evolutions on the lattice satisfy Lieb–Robinson bounds rather than strict locality. This motivates us to study approximately locality preserving unitaries (ALPUs). We show that the index theory is robust and completely extends to one-dimensional ALPUs. As a consequence, we achieve a converse to the Lieb–Robinson bounds: any ALPU of index zero can be exactly generated by some time-dependent, quasi-local Hamiltonian in constant time. For the special case of finite chains with open boundaries, any unitary satisfying the Lieb–Robinson bound may be generated by such a Hamiltonian. We also discuss some results on the stability of operator algebras which may be of independent interest.
Proteomic analysis of hepatic effects of phenobarbital in mice with humanized liver
Authors: Sprenger Heike , Rasinger Josef Daniel , Hammer Helen , Naboulsi Wael , Zabinsky Elke , Planatscher Hannes , Schwarz Michael , Poetz Oliver , Braeuning Albert 
Published: July 2022

AbstractActivation of the constitutive androstane receptor (CAR) may induce adaptive but also adverse effects in rodent liver, including the induction of drug-metabolizing enzymes, transient hepatocellular proliferation, and promotion of liver tumor growth. Human relevance of CAR-related adverse hepatic effects is controversially debated. Here, we used the chimeric FRG-KO mouse model with livers largely repopulated by human hepatocytes, in order to study human hepatocytes and their response to treatment with the model CAR activator phenobarbital (PB) in vivo. Mice received an intraperitoneal injection with 50 mg/kg body weight PB or saline, and were sacrificed after 72–144 h. Non-repopulated FRG-KO mice were used as additional control. Comprehensive proteomics datasets were generated by merging data obtained by targeted as well as non-targeted proteomics approaches. For the first time, a novel proteomics workflow was established to comparatively analyze the effects of PB on human and murine proteins within one sample. Analysis of merged proteome data sets and bioinformatics data mining revealed comparable responses in murine and human hepatocytes with respect to nuclear receptor activation and induction of xenobiotic metabolism. By contrast, activation of MYC, a key regulator of proliferation, was predicted only for mouse but not human hepatocytes. Analyses of 5-bromo-2′-deoxyuridine incorporation confirmed this finding. In summary, this study for the first time presents a comprehensive proteomic analysis of CAR-dependent effects in human and mouse hepatocytes from humanized FRG-KO mice. The data support the hypothesis that PB does induce adaptive metabolic responses, but not hepatocellular proliferation in human hepatocytes in vivo.
[1,2,4] Triazolo [3,4-a]isoquinoline chalcone derivative exhibits anticancer activity via induction of oxidative stress, DNA damage, and apoptosis in Ehrlich solid carcinoma-bearing mice
Authors: WalyEldeen Amr Ahmed , El-Shorbagy Haidan M. , Hassaneen Hamdi M. , Abdelhamid Ismail A. , Sabet Salwa , Ibrahim Sherif Abdelaziz 
Published: July 2022

Abstract
Despite the advances made in cancer therapeutics, their adverse effects remain a major concern, putting safer therapeutic options in high demand. Since chalcones, a group of flavonoids and isoflavonoids, act as promising anticancer agents, we aimed to evaluate the in vivo anticancer activity of a synthetic isoquinoline chalcone (CHE) in a mice model with Ehrlich solid carcinoma. Our in vivo pilot experiments revealed that the maximum tolerated body weight-adjusted CHE dose was 428 mg/kg. Female BALB/c mice were inoculated with Ehrlich ascites carcinoma cells and randomly assigned to three different CHE doses administered intraperitoneally (IP; 107, 214, and 321 mg/kg) twice a week for two consecutive weeks. A group injected with doxorubicin (DOX; 4 mg/kg IP) was used as a positive control. We found that in CHE-treated groups: (1) tumor weight was significantly decreased; (2) the total antioxidant concentration was substantially depleted in tumor tissues, resulting in elevated oxidative stress and DNA damage evidenced through DNA fragmentation and comet assays; (3) pro-apoptotic genes p53 and Bax, assessed via qPCR, were significantly upregulated. Interestingly, CHE treatment reduced immunohistochemical staining of the proliferative marker ki67, whereas BAX was increased. Notably, histopathological examination indicated that unlike DOX, CHE treatment had minimal toxicity on the liver and kidney. In conclusion, CHE exerts antitumor activity via induction of oxidative stress and DNA damage that lead to apoptosis, making CHE a promising candidate for solid tumor therapy.
Hypohydration alters pre-frontal cortex haemodynamics, but does not impair motor learning
Authors: Goodman Stephen P. J. , Immink Maarten A. , Marino Frank E. 
Published: July 2022

AbstractIt is unknown how hypohydration influences fine motor performance training and motor learning. Here, 30 participants (aged 19–46 years) were randomly assigned to a hypohydration (HYPO) or control (CON) group (both n = 15). Moderate hypohydration (~ 2.4% loss in body mass) was produced in HYPO via active dehydration before a 46 min fluid restricted rest period was undertaken. The conclusion of rest coincided with when CON attended the facilities. Both groups undertook a discrete sequence production task consisting of 6 training blocks, and returned ~ 300 min later to complete a delayed retention and transfer test while euhydrated. Bilateral pre-frontal cortex (PFC) haemodynamics were assessed using functional near-infrared spectroscopy throughout training and delayed learning assessments. Response time improved across training (P < 0.01) and was similar between the groups (both P = 0.22). Analysis of training PFC haemodynamics revealed a significant group by block interaction for oxygenated (O2Hb; P < 0.01), but not deoxygenated haemoglobin (P = 0.77). In training block 1, bilateral O2Hb was higher in HYPO (P = 0.02), while bilateral O2Hb increased in CON between blocks 2–3 and 5–6 (both P ≤ 0.03). During the delayed retention and transfer test, no group differences or interactions were found in response time, response error, or PFC haemodynamics (all P ≥ 0.27). Moderate hypohydration does increase PFC activation during motor skill learning, however, this appears to be transient and of little consequence to training or delayed retention or transfer performance.
Use of apple pomace, glycerine, and potato wastewater for the production of propionic acid and vitamin B12
Authors: Piwowarek Kamil , Lipińska Edyta , Hać-Szymańczuk Elżbieta , Kolotylo Vitaliy , Kieliszek Marek 
Published: July 2022

Abstract
Propionic acid bacteria (PAB) are a source of valuable metabolites, including propionic acid and vitamin B12. Propionic acid, a food preservative, is synthesized from petroleum refining by-products, giving rise to ecological concerns. Due to changing food trends, the demand for vitamin B12 has been expected to increase in the future. Therefore, it is necessary to look for new, alternative methods of obtaining these compounds. This study was conducted with an aim of optimizing the production of PAB metabolites using only residues (apple pomace, waste glycerine, and potato wastewater), without any enzymatic or chemical pretreatment and enrichment. Media consisting of one, two, or three industrial side-streams were used for the production of PAB metabolites. The highest production of propionic acid was observed in the medium containing all three residues (8.15 g/L, yield: 0.48 g/g). In the same medium, the highest production of acetic acid was found — 2.31 g/L (0.13 g/g). The presence of waste glycerine in the media had a positive effect on the efficiency of propionic acid production and P/A ratio. The concentration of vitamin B12 obtained in the wet biomass of Propionibacterium freudenreichii DSM 20271 ranged from 90 to 290 µg/100 g. The highest production of cobalamin was achieved in potato wastewater and apple pomace, which may be a source of the precursors of vitamin B12 — cobalt and riboflavin. The results obtained show both propionic acid and vitamin B12 can be produced in a more sustainable manner through the fermentation of residues which are often not properly managed.

Key points
• The tested strain has been showed metabolic activity in the analyzed industrial side-streams (apple pomace, waste glycerine, potato wastewater).
• All the side-streams were relevant for the production of propinic acid.
• The addition of waste glycerine increases the propionic acid production efficiency and P/A ratio.
• B12 was produced the most in the media containing potato wastewater and apple pomace as dominant ingredients.
Comparison of the tumor immune microenvironment and checkpoint blockade biomarkers between stage III and IV non-small cell lung cancer
Authors: Gao Yinjie , Stein Michelle M. , Kase Matthew , Cummings Amy L. , Bharanikumar Ramit , Lau Denise , Garon Edward B. , Patel Sandip P. 
Published: July 2022

AbstractBackgroundAdjuvant immune checkpoint blockade (ICB) following chemoradiotherapy and adding ICB to chemotherapy have been key advances for stages III-IV non-small cell lung cancer (NSCLC) treatment. However, known biomarkers like PD-L1 are not consistently indicative of ICB response. Other markers within the tumor immune microenvironment (TIME) may better reflect ICB response and/or resistance mechanisms, but an understanding of how TIMEs differ between stage III and IV NSCLC has not been explored.MethodsReal-world data from unresectable, stage III-IV, non-squamous, pretreatment NSCLCs (stage IIIn = 106, stage IVn = 285) were retrospectively analyzed. PD-L1 immunohistochemistry (IHC) was compared toCD274gene expression. Then, differential gene expression levels, pathway enrichment, and immune infiltrate between stages were calculated from whole-transcriptome RNA-seq. Analyses were stratified byEGFRstatus.ResultsPD-L1 IHC andCD274expression in tumor cells were highly correlated (n = 295,P < 2.2e-16,⍴ = 0.74).CTLA4expression was significantly increased in stage III tumors (P = 1.32e-04), while no differences were observed for other ICB-related genes. Metabolic pathway activity was significantly enriched in stage IV tumors (P = 0.004), whereas several immune-related KEGG pathways were enriched in stage III. Stage IV tumors had significantly increased macrophage infiltration (P = 0.0214), and stage III tumors had a significantly higher proportion of CD4 + T cells (P = 0.017). CD4 + T cells were also relatively more abundant inEGFR-mutant tumors vs. wild-type (P = 0.0081).ConclusionDirectly comparing the TIMEs of stage III and IV NSCLC, these results carry implications for further studies of ICB response in non-resectable stage III NSCLC and guide further research of prognostic biomarkers and therapeutic targets.
Do anticoagulants impact the “in-house mortality” after surgical treatment of proximal femoral fractures—a multivariate analysis
Authors: Fenwick Annabel , Pfann Michael , Mayr Jakob , Antonovska Iana , Wiedl Andreas , Nuber Stefan , Förch Stefan , Mayr Edgar 
Published: July 2022

Abstract
Purpose
The prevalence of proximal femur fractures is increasing with rising population age. Patients are presenting with more comorbidities. Anticoagulants create a challenge for the necessary early surgical procedure (osteosynthesis or arthroplasty). Our aim was to investigate the influence of anticoagulants on in-house mortality after surgical treatment of proximal femoral fractures.

Methods
A retrospective single-centre study was conducted including 1933 patients with an average age of 79.8 years treated operatively for a proximal femoral fracture between January 2016 and June 2020. One treatment protocol was performed based on type of anticoagulant, surgery, and renal function. Patient data, surgical procedure, time to surgery, complications and mortality were assessed.

Results
On average, patients with anticoagulants had a delay to surgery of 41.37 hours vs 22.1 hours for patients without (p < 0.000). Anticoagulants were associated with the occurrence of complications. The total complication rate was 22.4%. Patients with complications showed a prolonged time to surgery in comparison to those without (28.9 h vs 24.9 h; p < 0.00). In-house mortality rate was 4% and twice as high for patients on anticoagulants (7.7%; p < 0.00). Whilst there was no significant difference in the mortality rate between surgery within 24 and 48 hours (2.9% vs. 3.8%; p < 0.535), there was a significant increase in mortality of patients waiting more than 48 hours (9.8%; p < 0.001).

Conclusions
Pre-existing anticoagulant therapy in patients with proximal femur fractures is associated with a higher mortality rate, risk of complications and prolonged hospital stay. Further influential factors are age, gender, BMI and time to surgery.
Atomic and microstructural origin of banded colours in purple-blue variety of agate from Yozgat Province, Turkey
Authors: Lorenzi Roberto , Zullino Andrea , Gagliardi Valentina , Prosperi Loredana , Paleari Alberto , Adamo Ilaria 
Published: July 2022

AbstractAgates from Yozgat province are appreciated on the gem market for their white and purple-blue banded colours. In this study, we present a detailed investigation aimed at the identification of the atomic and structural origin of this peculiar colouration of chalcedony. X-ray diffraction and Raman spectroscopy revealed the presence of fine grains of quartz and moganite with a preferential accumulation of the latter in the blue bands. Near-infrared diffuse absorption spectra show overtones of hydroxyls vibrations at 1425, 1900, and 2250 nm. In the visible, the broad absorption at about 500 nm, as well as its behaviour at low temperatures, is compatible with the optical activity of iron impurities in quartz matrices, such as that observed in amethysts. Peak intensities and shapes are very similar for spectra collected in blue and white bands. Accordingly, trace-element composition from laser ablation inductively coupled mass spectrometry confirmed that the two regions have similar Fe content. The perceived changes in band colours are indeed originated by differences in microstructural arrangement and size of the grains visualised through scanning electron microscopy. White and blue stripes have grains of about 5 µm and 300 nm in size, respectively, resulting in an accentuated scattering component for the white bands. Therefore, the unique purple-blue shades typical of Yozgat agates are a combination of iron-related colour centres and scattering effect.
UMOD and the architecture of kidney disease
Authors: Devuyst Olivier , Bochud Murielle , Olinger Eric 
Published: July 2022

AbstractThe identification of genetic factors associated with the risk, onset, and progression of kidney disease has the potential to provide mechanistic insights and therapeutic perspectives. In less than two decades, technological advances yielded a trove of information on the genetic architecture of chronic kidney disease. The spectrum of genetic influence ranges from (ultra)rare variants with large effect size, involved in Mendelian diseases, to common variants, often non-coding and with small effect size, which contribute to polygenic diseases. Here, we review the paradigm of UMOD, the gene coding for uromodulin, to illustrate how a kidney-specific protein of major physiological importance is involved in a spectrum of kidney disorders. This new field of investigation illustrates the importance of genetic variation in the pathogenesis and prognosis of disease, with therapeutic implications.
Feasibility of “cold” triangle robotic pancreatoduodenectomy
Authors: Kauffmann Emanuele F. , Napoli Niccolò , Ginesini Michael , Gianfaldoni Cesare , Asta Fabio , Salamone Alice , Amorese Gabriella , Vistoli Fabio , Boggi Ugo 
Published: July 2022

Abstract
Background
Triangle pancreatoduodenectomy adds to the conventional procedure the en bloc removal of the retroperitoneal lympho-neural tissue included in the triangular area bounded by the common hepatic artery (CHA), the superior mesenteric artery (SMA), and the superior mesenteric vein/portal vein. We herein aim to show the feasibility of “cold” triangle robotic pancreaticoduodenectomy (C-Tr-RPD) for pancreatic cancer (PDAC).

Methods
Cold dissection corresponds to sharp arterial divestment performed using only the tips of robotic scissors. After division of the gastroduodenal artery, triangle dissection begins by lateral-to-medial divestment of the CHA and anterior-to-posterior clearance of the right side of the celiac trunk. Next, after a wide Kocher maneuver, the origin of the SMA, and the celiac trunk are identified. After mobilization of the first jejunal loop and attached mesentery, the SMA is identified at the level of the first jejunal vein and is divested along the right margin working in a distal-to-proximal direction. Vein resection and reconstruction can be performed as required.
C-Tr-RPD was considered feasible if triangle dissection was successfully completed without conversion to open surgery or need to use energy devices. Postoperative complications and pathology results are presented in detail.

Results
One hundred twenty-seven consecutive C-Tr-RPDs were successfully performed. There were three conversions to open surgery (2.3%), because of pneumoperitoneum intolerance (n = 2) and difficult digestive reconstruction. Thirty-four patients (26.7%) required associated vascular procedures. No pseudoaneurysm of the gastroduodenal artery was observed. Twenty-eight patients (22.0%) developed severe postoperative complications (≥ grade III). Overall 90-day mortality was 7.1%, declining to 2.3% after completion of the learning curve. The median number of examined lymph nodes was 42 (33–51). The rate of R1 resection (7 margins < 1 mm) was 44.1%.

Conclusion
C-Tr-RPD is feasible, carries a risk of surgical complications commensurate to the magnitude of the procedure, and improves staging of PDAC.

Graphical abstract
The inverse-electron demand Diels–Alder reaction of tetrazines with cyclic enol ethers
Authors: Schafzahl Bettina , Knall Astrid C. , Slugovc Christian 
Published: July 2022

AbstractInverse electron-demand Diels–Alder additions (iEDDA) between 1,2,4,5-tetrazines and suitable unsaturated dienophiles such as olefins, alkynes, or enol ethers provide facile access to pyridazines. Herein the use of cyclic enol ether derivatives for preparing pyridazines bearing 2-hydroxyethyl, 3-hyproxypropyl, and 3-oxopropyl substituents at the 4-position is disclosed and second order rate constants for the reactions with 2,3-dihydrofuran, 3,4-dihydro-2H-pyran, and 2-methoxy-3,4-dihydro-2H-pyran are presented.
Graphical abstract