bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Importance of Identifying Meibomian Gland Inversion in Patients With Floppy Eyelid Syndrome
Authors: Yvon Camille , Hunt Samantha , Malhotra Raman 
Published: September 2022

Background:
Floppy eyelid syndrome (FES) is a common and underdiagnosed condition characterized by eyelid hyperlaxity with reactive palpebral conjunctivitis that can cause ocular irritation. It may be associated with meibomian gland dysfunction (MGD) and secondary tarsal curling, resulting in upper eyelid meibomian gland inversion (MGI) in the absence of obvious marginal entropion.

Purpose:
To highlight the possible significance of MGI in patients with FES and report findings and outcomes in patients with concomitant MGI and FES undergoing correction of MGI with or without upper eyelid horizontal tightening.

Methods:
Retrospective, 5-year, noncomparative, single-center study of patients with FES and MGI, treated with MGI correction, with or without upper eyelid horizontal tightening, under the supervision of a single surgeon. Preoperative symptoms, surgical outcomes, complication rates, and postoperative symptoms were recorded.

Results:
A total of 13 eyes of 9 patients were treated with MGI surgery over the study period. Seven were male. Mean age at the surgery was 63 (range 42–81) years. Two OSs, 3 ODs, and 4 OUs were treated. All patients were “cotton-tip test” positive, and 77% (10/13) had MGI-related superior corneal fluorescein staining. Three patients (33%) had previous standard tightening procedures with recurrence of symptoms within 5 to 24 (mean 16) months. Repeat horizontal tightening had been considered in all these cases before referral to our unit. Mean follow-up was 20 months. Eight patients (88.9%) had improvement of symptoms (n = 3, full resolution; n = 5, partial resolution). All patients demonstrated restoration of the normal anatomical position of the meibomian glands. Superior punctate staining resolved in all eyes.

Conclusion:
This study provides a proof of concept that upper eyelid MGI can be present and symptomatic in patients with FES. It may help explain cases where symptoms persist or recur early following standard upper eyelid horizontal tightening. Where superior corneal punctate staining and a positive cotton-tip test exist, surgical correction of MGI, alongside horizontal tightening, may provide better, and longer-lasting symptomatic relief. This study provides evidence for the need for a prospective study to evaluate the contribution of MGI in patients with FES.
Patient Participation in Forensic Psychiatric Care
Authors: Selvin Mikael , Almqvist Kjerstin , Fogelkvist Maria , Lundqvist Lars-Olov , Schröder Agneta 
Published: September 2022

ABSTRACT
Patient participation is central in modern health care. However, it is a complex phenomenon that lacks a clear definition, and what constitutes participation varies depending on the context and theoretical perspective. It is known that patient participation in forensic psychiatric care is often rated as low by both patients and professionals, and it can be assumed that interventions to increase it are beneficial. In this process, management and staff could benefit from assessing perceived patient participation, and reliable and valid measurement instruments are essential. The aim of this study was to develop an instrument that could be used to measure experiences of participation in forensic psychiatric care from a patient perspective and test it for content validity. A definition of patient participation in forensic psychiatric care was formulated and operationalized in an instrument that an expert group, consisting of patients with ongoing care, evaluated for content validity. In total, 50 items were sorted into five different dimensions: to have good communication, to be involved, to have mutual trust, to trust the care, and to take responsibility. After psychometric testing, the instrument has the potential to become a tool to use in research, clinical work, and development work in the field of forensic psychiatric care. In addition to being used as a measure, the Patient Participation in Forensic Psychiatric Care can also be used to encourage a dialogue about their care and to make both patients and staff more aware of patient participation.
Solution-related in Vitro Dewetting Behavior of Various Daily Disposable Contact Lenses
Authors: Marx Sebastian , Baluschev Stanislav , Sickenberger Wolfgang 
Published: September 2022

SIGNIFICANCE
The dewetting process of contact lenses (CLs) is a result of material and solution properties as well as environmental factors. This article describes an investigational approach to observe and describe dewetting characteristics of different CL material and solution combinations.

PURPOSE
This study aimed to determine the in vitro dewetting characteristics of various daily disposable CLs that were assessed using a noninvasive keratograph dewetting procedure (noninvasive keratograph dry-up time). In vitro dewetting data of the same CL materials soaked in saline solution and artificial tear solution (ATS) were measured to determine additional dewetting characteristics.

METHODS
Noninvasive keratograph dry-up time was measured for six different soft CL materials and three different test conditions, in their specific blister solution, after exposure to saline and an ATS. Twenty CLs of each solution/material combination were assessed after an 8-hour soaking, during a 180-second dewetting observation, and the results were expressed by area under the curve values.

RESULTS
Fastest dewetting occurred for all materials when measured out of saline, indicated by the highest averaged area under the curve value of 9243.3 ± 38.3 over all lens materials. Slower dewetting was detected for all materials when measured out of their specific blister solution (7755.9 ± 37.1) and out of ATS (7988.8 ± 40.0). Intragroup results were statistically significantly different for all solutions showing the smallest differences within the ATS group (P < .001, Kruskal-Wallis test).

CONCLUSIONS
A pure saline thin film is not an ideal representation of a complex tear film layer of a healthy human because it lacks any evaporative protection by a lipid layer. The use of an ATS, which more likely mimics the natural tear film, allowed in this experimental in vitro project to decrease the gap to the in vivo field. In vitro dewetting information in connection with the blister solution allows only a theoretical conclusion about the initial lens wear after lens insertion.
Effects of GRIN2B, GRIA1, and BDNF Polymorphisms on the Therapeutic Action of Ketamine and Esketamine in Treatment-Resistant Depression Patients: Secondary Analysis From a Randomized Clinical Trial
Authors: Beanes Graziele , Caliman-Fontes Ana Teresa , Souza-Marques Breno , Silva Hátilla Dos Santos , Leal Gustavo C. , Carneiro Beatriz Alves , Guerreiro-Costa Lívia N.F. , Figueiredo Alexandre V. , Figueiredo Camila Alexandrina V. , Lacerda Acioly L.T. , Costa Ryan dos S. , Quarantini Lucas C. 
Published: September 2022

Objective
This study aimed to evaluate the effect of genetic variants in glutamate ionotropic receptor N-methyl-d-aspartate type subunit 2B (GRIN2B), glutamate ionotropic receptor α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid type subunit 1 (GRIA1), and brain-derived neurotrophic factor (BDNF) genes on therapeutic response, remission, and total Montgomery-Åsberg Depression Rating Scale scores after treatment with ketamine or esketamine in treatment-resistant depression (TRD) patients.

Methods
Participants (N = 60) are from a double-blind, randomized, noninferiority clinical trial comparing single-dose intravenous ketamine (0.5 mg/kg) to esketamine (0.25 mg/kg) for TRD. Montgomery-Åsberg Depression Rating Scale was applied at baseline, 24 hours, 72 hours, and 7 days postinfusion to assess depressive symptoms. Blood samples were collected to evaluate single nucleotide polymorphisms rs1805502 (GRIN2B), rs1994862 (GRIA1), and rs6265 (BDNF).

Results
There was no association between rs1805502, rs1994862, or rs6265 polymorphisms and antidepressant response (P = 0.909, P = 0.776, and P = 0.482, respectively), remission P = 0.790, P = 0.086, and P = 0.669), or Montgomery-Åsberg Depression Rating Scale scores at each time point (P = 0.907, P = 0.552, and P = 0.778).

Conclusions
We found no association between the studied single nucleotide polymorphisms (rs6265, rs1805502, and rs1994862) and ketamine's therapeutic action in TRD patients. Further studies with larger samples are needed to clarify the utility of these genes of interest as predictors for antidepressant treatment.
The Effects of Disease-Modifying Therapies on Oxidative Stress in Patients With Relapsing-Remitting Multiple Sclerosis
Authors: Topic Aleksandra , Vasic Marija , Markovic Bojan , Milinkovic Neda , Dincic Evica 
Published: September 2022

Objective
Oxidative stress (OS) has a role in the pathogenesis and progression of multiple sclerosis. The effects of disease-modifying therapies (DMTs) on OS are unclear. We aimed to explore the association between DMTs and OS in patients with relapsing-remitting multiple sclerosis (RRMS).

Methods
The study conducted in 167 patients (102 received and 65 not received the DMTs). The DMTs included interferon beta-1a (n = 15), interferon beta-1b (n = 20), glatiramer acetate (n = 10), and sphingosine-1-phosphate receptor modulators (n = 57). Oxidative stress assessed by total antioxidant status (TAS) and total oxidant status (TOS) (determined by spectrophotometric method), oxidative index (OSI was calculated), and urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG/creatinine was determined by high-performance liquid chromatography and tandem mass spectrometry). Patients were classified by Multiple Sclerosis Severity Score (MSSS) to mild/moderate (MSSS, <6.7) and severe (MSSS, >6.7).

Results
Disease-modifying therapies are associated with increased TAS, decreased TOS, OSI, and 8-oxodG/creatinine. Regardless of therapy, women had a less favorable redox status (lower TAS, higher TOS and OSI). Patients with MSSS>6.7 and without DMTs had higher OSI than patients who received DMTs. Women with MSSS>6.7 without DMTs had lower TAS than women with DMTs, whereas in the same stage of MS, men without DMTs had higher TOS than patients with DMTs. Women with MSSS<6.7 and with DMTs had lower 8-oxodG/creatinine compared with those without DMT therapy.

Conclusions
The antioxidant effects of DMTs were evidenced in this study. The gender-related effects of DMTs on the OS imply the personalized antioxidant pharmacotherapy, especially for the women. The OS biomarkers have a potential as the prognostic for the assessment of DMTs outcomes in patients with RRMS.
Aspects of flow variability and spatial context predict temporal beta diversity in river metacommunities
Authors: Saffarinia P. , Conway R. , Anderson K.E 
Published: September 2022

AbstractRiver catchments are dynamic networks that contain multiple levels of spatial and temporal complexity. Benthic macroinvertebrates are key indicator taxa throughout catchments and beta diversity has been used as a metric to explore determinants of community composition at the catchment scale. Commonly explored drivers of beta diversity include environmental and spatial variables such as flow, temperature, and spatial distance. While factors influencing spatial beta diversity have been explored, factors explaining temporal beta diversity have been understudied. Temporal beta diversity is predicted to also be important since community assembly mechanisms are not stable over time, and more studies are needed to determine which factors most strongly determine temporal beta diversity patterns.We investigated the effects of local environmental variables, flow variability, and spatial context on temporal beta-diversity using a large, publicly available biomonitoring dataset from river networks in California. Data included benthic macroinvertebrate community composition and environmental data from multiple locations and years, allowing us to explore temporal changes in these communities as a function of site-specific environmental and spatial factors. Associated gage data were used to calculate hydrograph metrics and contextualize the flow regime at each location over long timescales. We then used beta regression to model the relationship between benthic macroinvertebrate temporal beta-diversity, environmental variables, flow regimes, and spatial network context.Flow and spatial catchment-related predictors were the strongest predictors of temporal beta diversity, while changes in environmental variables were much weaker. Channel slope, drainage density, and upstream catchment area were the most significant spatial factors. Channel slope showed a negative relationship with temporal beta diversity, while drainage density and upstream catchment area showed positive ones. Temporal beta diversity was also higher when the rate and magnitude of rises and falls in flow was higher in the hydrograph as well as when the number of zero-flow days and the duration of flow rises and falls was higher.Overall, our results indicate that temporal beta diversity of freshwater benthic macroinvertebrates is shaped by both long-term hydrological context and spatial context, and that these factors may serve as better predictors of long term community variability than variability in point estimates of environmental measurements. Flow regimes and spatial metrics may provide more environmental context than point-estimate environmental measurements, as the latter may not accurately capture the dynamic conditions that drive variability in metacommunity responses.Our study supports the need for biomonitoring efforts at long spatial and temporal timescales, and highlights the need to consider metacommunity change in the management of freshwater systems.
Natural variation in codon bias and mRNA folding strength interact synergistically to modify protein expression in Saccharomyces cerevisiae
Authors: Wienecke Anastacia N. , Barry Margaret L. , Pollard Daniel A. 
Published: September 2022

AbstractCodon bias and mRNA folding strength (mF) are hypothesized molecular mechanisms by which polymorphisms in genes modify protein expression. Natural patterns of codon bias and mF across genes as well as effects of altering codon bias and mF suggest the influence of these two mechanisms may vary depending on the specific location of polymorphisms within a transcript. Despite the central role codon bias and mF may play in natural trait variation within populations, systematic studies of how polymorphic codon bias and mF relate to protein expression variation are lacking. To address this need, we analyzed genomic, transcriptomic, and proteomic data for 22 Saccharomyces cerevisiae isolates, estimated protein accumulation for each allele of 1620 genes as the log of protein molecules per RNA molecule (logPPR), and built linear mixed effects models associating allelic variation in codon bias and mF with allelic variation in logPPR. We found codon bias and mF interact synergistically in a positive association with logPPR and this interaction explains almost all the effect of codon bias and mF. We examined how the locations of polymorphisms within transcripts influence their effects and found that codon bias primarily acts through polymorphisms in domain encoding and 3’ coding sequences while mF acts most significantly through coding sequences with weaker effects from UTRs. Our results present the most comprehensive characterization to date of how polymorphisms in transcripts influence protein expression.
Identifying opportunities for high resolution pesticide usage data to improve the efficiency of endangered species pesticide risk assessment
Authors: Eikenberry Steffen E. , Iacona Gwen , Murphy Erin L. , Watson Greg , Gerber Leah R. 
Published: September 2022

AbstractThe US pesticide registration and review process requires regular re-assessment of the risk of pesticide use, including risk to species listed under the Endangered Species Act (ESA). Yet, current methods for assessment were not designed to assess threats that could involve hundreds of pesticides potentially impacting multiple species across a continent. Thus, many pesticides remain on the market without complete review under ESA. A promising approach to address this challenge calls for the use of high-resolution data in the spatial analyses used to determine the likelihood of adverse effect. However, such data are rare, and implementing data collection initiatives are difficult and expensive. Here we examine the extent to which increased data resolution could result in significant gains in efficiency to the pesticide risk assessment process. To do so, we employ a modified value-of-information (VOI) approach. By using data available only in California, we found that high resolution data increased the number of species deemed not likely to be adversely affected by pesticides from less than 5% to nearly 50%. Across the contiguous US, we predicted that 48% of species would be deemed not likely to be adversely affected using high resolution data, compared to 20% without. However, if such data were available in just 11 states, 68% of the available gains in efficiency could be obtained. Therefore, a focused collection or release of high-resolution pesticide usage data could expedite pesticide risk assessment and reduce endangered species’ risk of pesticide impacts.SignificanceRegulation of environmental risk must balance avoidance of harm with assessment efficiency. The current pesticide registration and review process in the U.S. is generally considered ineffective in assessing impact on species listed under the Endangered Species Act, due to the vast scale of the problem. We assessed the value of using high resolution pesticide usage data in the risk assessment process to identify cost-effective opportunities to rapidly improve this assessment process and ensure that ESA listed species are protected while accounting for agricultural production. Our results suggest that using existing high-resolution data in California and collection of such information from 11 other states could reduce the risk assessment burden across the contiguous U.S. by one-quarter.
Neogene climatic fluctuations and poor connectivity with the centres of diversity shaped the Western Palearctic net-winged beetle fauna
Authors: Motyka Michal , Kusy Dominik , Bilkova Renata , Bocak Ladislav 
Published: September 2022

AbstractOnly twenty-two net-winged beetle species (Elateroidea: Lycidae) are known from the Western Palearctic region (WPR), i.e., less than 0.5% of the global lycid diversity and much fewer than from the similar latitudes of East Asia or Northern America. We use the comprehensive distribution data and the molecular phylogeny of ∼400 world lycids, including fourteen European species, to provide a new perspective for understanding the structure and evolution of this group in the WPR. All Mediterranean species represent deeply rooted lineages with relatives in Eastern Asia. These species occur in relictual ranges close to the family’s Pleistocene refugial edge. The phylogeny points to the loss of biological connection with East Asia since the Mid Miocene. A third of WPR species is widespread in Central and Northern Europe, reaching Eastern Asia, some of them possibly younger elements of the European fauna. Unlike relatively high diversity in the Eocene amber, the extant net-winged beetles represent a small fraction of elateroid diversity in the WPR and are generally rare. Therefore, we assume that most WPR species are relics trapped in Mediterranean refugia since the onset of the Plio-Pleistocene cooling and are critically endangered by the ongoing loss of suitable habitats.
Cyanotoxins accumulate in Lake St. Clair fish yet their fillets are safe to eat
Authors: Shahmohamadloo René S. , Bhavsar Satyendra P. , Almirall Xavier Ortiz , Marklevitz Stephen A. C. , Rudman Seth M. , Sibley Paul K. 
Published: September 2022

AbstractConsuming fish exposed to cyanobacterial harmful algal blooms (HABs) may be a major route of microcystin toxin exposure to humans. However, it remains unknown whether fish can accumulate and retain microcystins temporally in waterbodies with recurring seasonal HABs, particularly before and after a HAB event when fishing is active. We conducted a field study on Largemouth Bass, Northern Pike, Smallmouth Bass, Rock Bass, Walleye, White Bass, and Yellow Perch to assess the human health risks to microcystin toxicity via fish consumption. We collected 124 fish in 2016 and 2018 from Lake St. Clair, a large freshwater ecosystem in the North American Great Lakes that is actively fished pre- and post-HAB periods. Muscles were analyzed using the MMPB Lemieux Oxidation method for total microcystins, which was used to perform a human health risk assessment for comparison against fish consumption advisory benchmarks available for Lake St. Clair. From this collection 35 fish livers were additionally extracted to confirm the presence of microcystins. Microcystins were detected in all livers at widely varying concentrations (1-1,500 ng g-1ww), suggesting HABs are an underappreciated and pervasive stressor to fish populations. Conversely, microcystin levels were consistently low in muscles (0-15 ng g-1ww) and presented negligible risk, empirically supporting that fillets may be safely consumed before and after HAB events following fish consumption advisories.
Embryonic Vitamin D Deficiency Programs Hematopoietic Stem Cells to Induce Type 2 Diabetes
Authors: Oh Jisu , Riek Amy E. , Bauerle Kevin T. , Dusso Adriana , McNerney Kyle P. , Barve Ruteja A. , Darwech Isra , Sprague Jennifer , Moynihan Clare , Zhang Rong M , Wang Ting , Xing Xiaoyun , Li Daofeng , Head Richard D. , Bambouskova Monika , Mrad Marguerite , Collins Alejandro , Sands Mark S. , Bernal-Mizrachi Carlos 
Published: September 2022

AbstractEnvironmental factors may alter the fetal genome to cause metabolic diseases. It is unknown whether embryonic immune cell programming impacts the risk of type 2 diabetes in later life. We demonstrate that transplantation of fetal hematopoietic stem cells (HSCs) made vitamin D deficient in utero induces diabetes in vitamin D-sufficient mice. Vitamin D deficiency epigenetically suppresses Jarid2 expression and activates the Mef2/PGC1a pathway in HSCs, which persists in recipient bone marrow, resulting in adipose macrophage infiltration. These macrophages secrete miR106-5p, which promotes adipose insulin resistance by repressing PIK3 catalytic subunit alpha and AKT signaling. Vitamin D-deficient monocytes from human cord blood have comparable Jarid2/Mef2/PGC1a expression changes and secrete miR-106b-5p, causing adipocyte insulin resistance. These findings suggest that vitamin D deficiency during development has epigenetic consequences impacting the systemic metabolic milieu.