bluuebunny/crossref_metadata_2025_split huggingface dataset:
A millimeter-scale insight into formation mechanism of lacustrine black shale in tephra deposition background
Authors: Lin Senhu , Hou Lianhua , Luo Xia , Wu Yiwen 
Published: July 2022

AbstractTo reveal the role of tephra in the deposition of black shale during periods of volcanic activity, we performed lithostratigraphic and geochemical analyses on 14 horizontally sliced samples drilled from a 2-cm-thick black shale interval in the lower Ch7 Member of the Upper Triassic Yanchang Formation, southern Ordos Basin. Results indicate that fewer plankton is preserved during tephra deposition than during periods of volcanic quiescence. With the decrease of volcanic activities and tephra deposition, the abundance of redox-sensitive trace elements (RSTEs) and biolimiting elements increases, while terrigenous elements gradually decrease, resulting in the improvement of organic matter (OM) preservation. Paleoenvironmental proxies suggest that the climate during the Late Triassic was generally warm and humid. However, subsequent intense volcanic eruptions may have caused climatic cooling that affected the water column, resulting in enhanced salinity, primary production, water stratification, and bottom water anoxia, leading to enhanced organic carbon production and preservation. Primary productivity and redox conditions controlled the accumulation of organic carbon. Although physical and chemical reactions relating to the deposition of tephra into water are short-lived, climate change induced by volcanic eruptions is the critical cause of black shale formation.
Relationship between drug holiday of the antiresorptive agents and surgical outcome of medication-related osteonecrosis of the jaw in osteoporosis patients
Authors: Morishita Kota , Soutome Sakiko , Otsuru Mitsunobu , Hayashida Saki , Murata Maho , Sasaki Miho , Takagi Yukinori , Sumi Misa , Umeda Masahiro 
Published: July 2022

AbstractA drug holiday of 3 months does not promote separation of sequestra and is not correlated with treatment outcomes after surgical therapy in osteoporosis patients who receive antiresorptive agents and who have medication-related osteonecrosis of the jaw. Medication-related osteonecrosis of the jaw (MRONJ) is a serious adverse effect of antiresorptive agents alone or in combination with immune modulators or antiangiogenic medications, in the absence of radiation exposure to the head and neck region. The effectiveness of surgical treatment for MRONJ has been reported, but the timing of the operation remains controversial. The purpose of this study was to clarify whether preoperative drug holidays of antiresorptive agents promote sequestrum separation and improve treatment outcomes in patients who receive low doses of antiresorptive agents. This retrospective study included 173 patients who received low-dose antiresorptive agents and underwent surgical therapy. The effects of a drug holiday on the separation of sequestra and treatment outcomes were analyzed using logistic and Cox regression analyses. Multivariate analysis revealed that administration of an antiresorptive agent for more than 4 years, a high number of lymphocytes, and an extensive osteolytic area were significantly correlated with separation of sequestra, but drug holiday did not promote sequestrum separation. Furthermore, a drug holiday of 90, 120 or 180 days did not show any improvement in treatment outcomes. The drug holiday of the antiresorptive agents for the treatment of MRONJ is unnecessary, and surgical therapy should be performed early.
Characterization of regulatory transcriptional mechanisms in hepatocyte lipotoxicity
Authors: Pérez-Schindler Joaquín , Vargas-Fernández Elyzabeth , Karrer-Cardel Bettina , Ritz Danilo , Schmidt Alexander , Handschin Christoph 
Published: July 2022

AbstractNon-alcoholic fatty liver disease is a continuum of disorders among which non-alcoholic steatohepatitis (NASH) is particularly associated with a negative prognosis. Hepatocyte lipotoxicity is one of the main pathogenic factors of liver fibrosis and NASH. However, the molecular mechanisms regulating this process are poorly understood. The main aim of this study was to dissect transcriptional mechanisms regulated by lipotoxicity in hepatocytes. We achieved this aim by combining transcriptomic, proteomic and chromatin accessibility analyses from human liver and mouse hepatocytes. This integrative approach revealed several transcription factor networks deregulated by NASH and lipotoxicity. To validate these predictions, genetic deletion of the transcription factors MAFK and TCF4 was performed, resulting in hepatocytes that were better protected against saturated fatty acid oversupply. MAFK- and TCF4-regulated gene expression profiles suggest a mitigating effect against cell stress, while promoting cell survival and growth. Moreover, in the context of lipotoxicity, some MAFK and TCF4 target genes were to the corresponding differentially regulated transcripts in human liver fibrosis. Collectively, our findings comprehensively profile the transcriptional response to lipotoxicity in hepatocytes, revealing new molecular insights and providing a valuable resource for future endeavours to tackle the molecular mechanisms of NASH.
Comparative transcriptome analysis of synthetic and common wheat in response to salt stress
Authors: Nakayama Rio , Safi Mohammad Taheb , Ahmadzai Waisuddin , Sato Kazuhiro , Kawaura Kanako 
Published: July 2022

AbstractSalt stress reduces wheat yield. Therefore, improvement for enhanced salt stress tolerance is necessary for stable production. To understand the molecular mechanism of salt tolerance in common wheat and synthetic hexaploid (SH) wheat, RNA sequencing was performed on the roots of three wheat lines salt-tolerant SH wheat, salt-tolerant common wheat, and salt-sensitive common wheat. Differentially expressed genes (DEGs) in response to salt stress were characterized using gene ontology enrichment analysis. Salt tolerance in common wheat has been suggested to be mainly regulated by the activation of transporters. In contrast, salt tolerance in SH wheat is enhanced through up-regulation of the reactive oxygen species signaling pathway, other unknown pathways, and different ERF transcription factors. These results indicate that salt tolerance is differentially controlled between common wheat and SH wheat. Furthermore, QTL analysis was performed using the F2 population derived from SH and salt-sensitive wheat. No statistically significant QTL was detected, suggesting that numerous QTLs with negligible contributions are involved in salt tolerance in SH wheat. We also identified DEGs specific to each line near one probable QTL. These findings show that SH wheat possesses salt tolerance mechanisms lacking in common wheat and may be potential breeding material for salt tolerance.
The combined effects of Map3k1 mutation and dioxin on differentiation of keratinocytes derived from mouse embryonic stem cells
Authors: Wang Jingjing , Xiao Bo , Kimura Eiki , Mongan Maureen , Xia Ying 
Published: July 2022

AbstractEpithelial development starts with stem cell commitment to ectoderm followed by differentiation to the basal keratinocytes. The basal keratinocytes, first committed in embryogenesis, constitute the basal layer of the epidermis. They have robust proliferation and differentiation potential and are responsible for epidermal expansion, maintenance and regeneration. We generated basal epithelial cells in vitro through differentiation of mouse embryonic stem cells (mESCs). Early on in differentiation, the expression of stem cell markers, Oct4 and Nanog, decreased sharply along with increased ectoderm marker keratin (Krt) 18. Later on, Krt 18 expression was subdued when cells displayed basal keratinocyte characteristics, including regular polygonal shape, adherent and tight junctions and Krt 14 expression. These cells additionally expressed abundant Sca-1, Krt15 and p63, suggesting epidermal progenitor characteristics. Using Map3k1 mutant mESCs and environmental dioxin, we examined the gene and environment effects on differentiation. Neither Map3k1 mutation nor dioxin altered mESC differentiation to ectoderm and basal keratinocytes, but they, individually and in combination, potentiated Krt 1 expression and basal to spinous differentiation. Similar gene-environment effects were observed in vivo where dioxin exposure increased Krt 1 more substantially in the epithelium of Map3k1+/- than wild type embryos. Thus, the in vitro model of epithelial differentiation can be used to investigate the effects of genetic and environmental factors on epidermal development.
Study on deterioration mechanism of soil in Zhouqiao site under salinization
Authors: Yue Jianwei , Gao Huijie , Zhao Limin , Kong Qingmei , Xu Xiangchun , Wang Zifa , Chen Ying 
Published: July 2022

AbstractAlkalinity production is one of the most typical and widespread salinization hazards on the Loess Plateau. Based on the characterization of typical flooding sites and the results of salt monitoring, this study investigates the deterioration mechanism of salinization on Zhouqiao site. The orthogonal test was used to simulate the effects of different concentrations of MgSO4, NaCl and CaCl2 under natural conditions on the quality change, salt analysis out location, surface phenomenon, strength and electrical conductivity of the soil at the Zhouqiao site, and to make a preliminary analysis on the mechanism of saline deterioration of the site soil. The results show that the soil column mass increased significantly under the action of salt, and the rate of salt absorption in the soil column decreased when the critical value was reached, and the critical values were different under the action of different kinds of salts. The rate of salt analysis is also influenced by the salt concentration and the number of cycles, which gradually increases with the increase of salt concentration and the number of cycles. The nominal strength of the soil column with the number of cycles, but occasionally increases. The conductivity increases with the number of cycles, and the magnitude distribution of the conductivity of the soil column under the action of different salts is not exactly the same.
Synchronous intracellular delivery of EGFR-targeted antibody–drug conjugates by p38-mediated non-canonical endocytosis
Authors: Takahashi Jun-ichiro , Nakamura Shiori , Onuma Iimi , Zhou Yue , Yokoyama Satoru , Sakurai Hiroaki 
Published: July 2022

AbstractMonoclonal antibodies targeting the epidermal growth factor receptor (EGFR), including cetuximab and panitumumab, have been used in clinic settings to treat cancer. They have also recently been applied to antibody–drug conjugates (ADCs); however, their clinical efficacy is limited by several issues, including lower internalization efficiency. The binding of cetuximab to the extracellular domain of EGFR suppresses ligand-induced events; therefore, we focus on ligand-independent non-canonical EGFR endocytosis for the delivery of ADCs into cells. Tumor necrosis factor-α (TNF-α) strongly induces the endocytosis of the cetuximab-EGFR complex within 15 min via the p38 phosphorylation of EGFR in a tyrosine kinase-independent manner. A secondary antibody conjugated with saporin, a ribosome-inactivating protein, also undergoes internalization with the complex and enhances its anti-proliferative activity. Anti-cancer agents, including cisplatin and temozolomide, also induce the p38-mediated internalization. The results of the present study demonstrate that synchronous non-canonical EGFR endocytosis may be a feasible strategy for promoting the therapeutic efficacy of EGFR-targeting ADCs in clinical settings.
Microwaves reduce water refractive index
Authors: Asakuma Yusuke , Maeda Tomoisa , Takai Takahiro , Hyde Anita , Phan Chi , Ito Shinya , Taue Shuji 
Published: July 2022

AbstractMicrowaves, long used as a convenient household appliance, have been increasingly used in industrial processes such as organic synthesis and oil processing. It has been proposed that microwaves can enhance these chemical processes via a non-thermal effect. Here we report the instantaneous effect of microwaves on the permittivity and phase velocity of light in water through the in-situ measurement of changes in refractive index. Microwave irradiation was found to reduce the water refractive index (RI) sharply. The reduction increased as a function of microwave power to a far greater extent than expected from the change in temperature. The phase velocity of light in water increases up to ~ 5% (RI of 1.27) during microwave irradiation. Upon stopping irradiation, the return to the equilibrium RI was delayed by up to 30 min. Our measurement shows that microwaves have a profound non-thermal and long-lasting effect on the properties of water. Further investigation is planned to verify if the observed RI reduction is restricted to the region near the surface or deep inside water bulk. The observation suggests a relationship between microwave-induced and the enhanced aqueous reactions.
Re-do Operation Using a Robotic System due to Locoregional Recurrence after Initial Thyroidectomy for Thyroid Cancer
Authors: Kim Dong Gyu , Kim Kwangsoon , Lee Ji-Eun , Ho Joon , Kim Jin Kyong , Kang Sang-Wook , Lee Jandee , Jeong Jong Ju , Nam Kee-Hyun , Chung Woong Youn 
Published: July 2022

AbstractLocoregional recurrent thyroid cancer is commonly treated with re-do operation. This study aimed to investigate the feasibility of using robotic system for re-do operation in locoregional recurrent thyroid cancer. Sixty-five patients who underwent re-do robotic operation using trans-axillary approach for locoregional recurrent thyroid cancer from October 2007 to April 2021 at Yonsei University Hospital were analyzed. Completion total thyroidectomy (CTT) was performed in 26 cases, CTT and modified radical neck node dissection (mRND) in 16, and mRND in 23. Most of the re-do robotic operations were performed at site of previous incision. All patients were diagnosed with papillary thyroid carcinoma (PTC). CTT with central compartment neck dissection (CCND) took 117.6 ± 26.3 min, CTT with mRND 255.6 ± 38.6 min, and mRND, 211.7 ± 52.9 min. Transient hypocalcemia occurred in 17 (26.2%) patients and permanent hypocalcemia occurred in 3 (4.6%). There was one case of recurrent laryngeal nerve(RLN) injury. One patient was diagnosed with structural recurrence after re-do robotic operation. Median follow-up duration was 50.7 ± 37.1 months. Re-do robotic operation can be an alternative for patients who are diagnosed with locoregional recurrent thyroid cancer after thyroidectomy, with no increase in morbidity, similar oncologic outcomes, and superior cosmetic satisfaction.
Theoretical stiffness limits of 4D printed self-folding metamaterials
Authors: van Manen Teunis , Dehabadi Vahid Moosabeiki , Saldívar Mauricio Cruz , Mirzaali Mohammad J. , Zadpoor Amir A. 
Published: July 2022

Abstract4D printing of flat sheets that self-fold into architected 3D structures is a powerful origami-inspired approach for the fabrication of multi-functional devices and metamaterials. However, the opposite stiffness requirements for the folding process and the subsequent loadbearing of 3D structures impose an intrinsic limitation in designing self-folding metamaterials: while a low stiffness is required for the successful completion of the self-folding step, a high stiffness is needed for utilizing the folded structure as a load-bearing mechanical metamaterial. Here, we present a nonlinear analytical model of self-folding bilayer constructs composed of an active and passive layer. This finite-deformation theoretical model predicts the curvature of activated bilayers, establishes their stability limits, and estimates the stiffness of folded bilayers, yielding the theoretical stiffness limits of self-folding bilayers. We use our model to identify the optimal combinations of geometrical and mechanical properties that result in the highest possible stiffness of folded constructs. We then compare the predictions of our analytical model with computational results, and validate our theory with experimental realizations of 4D printed structures. Finally, we evaluate the theoretical stiffness limits of bilayer constructs made using the most common types of stimuli-responsive materials. Our analysis shows that a maximum effective modulus of ≈ 1.5 GPa can be achieved using the currently available shape-memory polymers.
Knowledge engineering–based DApp using blockchain technology for protract medical certificates privacy
Authors: Rupa Chiramdasu , MidhunChakkarvarthy Divya , Patan Rizwan , Prakash Ande Bhanu , Pradeep Ghantasala G. S. 
Published: July 2022

AbstractIn the Industry 4.0 era, an inherited featured technology, blockchain, plays a vital role in knowledge engineering applications. Blockchain provides privacy to sensitive data as an intelligent agent, so its adoption rate increases in all the advanced domains. Especially in the health care department, blockchain technology usage helps avoid attacks like the Wannacry ransomware attack during 2017. Therefore, this paper described a decentralised application (DApp) expert system using public blockchain to create and maintain official health documents, especially medical certificates. Current existing systems, either paper‐based or database or clouds to save the medical certificates, have more scope to do attacks. Hence, proposed a blockchain‐based DApp that acts as an interface between intelligent agents, blockchains, and system related to the medical certificates. The main strength of this paper is implementation results, which are not among the maximum literary works currently available. The associate cost for conducting distributed application operations on the blockchain in terms of Gas comprehensively presented here. Furthermore, it consists of comparing the system's non‐functional functions by considering blockchain and non‐blockchain environments. Also, presented the simulation results with the performance results compared with the existed systems.
Dependent task offloading with energy‐latency tradeoff in mobile edge computing
Authors: Zhang Yanfang , Chen Jian , Zhou Yuchen , Yang Long , He Bingtao , Yang Yijin 
Published: July 2022

AbstractWith the rapid development of Internet‐of‐Things (IoT) and mobile devices, the IoT applications become more computation‐intensive and latency‐sensitive, which bring severe challenges to the resource‐limited devices. Mobile Edge Computing has served as a key promising method to enhance the network's computing capability by enabling resource‐constrained devices to offload tasks to the edge servers. A major challenge, which has been overlooked by most existing works on task offloading, is the dependencies among tasks and subtasks. In this paper, the subtask offloading with logical dependency for IoT applications is focused on. Specifically, subtask dependent graphs are employed to explore the dependency of subtasks and consider the priority of task scheduling. Further, an offloading scheme is put forward for minimizing both task latency and energy consumption of the device with dependency guarantees for all IoT tasks in multi‐server edge networks. Finaly, the simulation results demonstrate that the overall reduction rate is around 14% and relatively stable can
effectively reduce task latency in multi‐server edge networks.
Engineering of optogenetic devices for biomedical applications in mammalian synthetic biology
Authors: Guan Ningzi , Gao Xianyun , Ye Haifeng 
Published: July 2022

AbstractGene‐ and cell‐based therapies are the next frontiers in the field of medicine. Both are transformative and innovative therapies; however, a lack of safety data limits the translation of such promising technologies to the clinic. Improving the safety and promoting the clinical translation of these therapies can be achieved by tightly regulating the release and delivery of therapeutic outputs. In recent years, the rapid development of optogenetic technology has provided opportunities to develop precision‐controlled gene‐ and cell‐based therapies, in which light is introduced to precisely and spatiotemporally manipulate the behaviour of genes and cells. This review focuses on the development of optogenetic tools and their applications in biomedicine, including photoactivated genome engineering and phototherapy for diabetes and tumours. The prospects and challenges of optogenetic tools for future clinical applications are also discussed.
Distribution of solids in the rings of the HD 163296 disk: a multiwavelength study
Authors: Guidi G. , Isella A. , Testi L. , Chandler C. J. , Liu H. B. , Schmid H. M. , Rosotti G. , Meng C. , Jennings J. , Williams J. P. , Carpenter J. M. , de Gregorio-Monsalvo I. , Li H. , Liu S. F. , Ortolani S. , Quanz S. P. , Ricci L. , Tazzari M. 
Published: July 2022

Context. Observations at millimeter wavelengths of bright protoplanetary disks have shown the ubiquitous presence of structures such as rings and spirals in the continuum emission. The derivation of the underlying properties of the emitting material is nontrivial because of the complex radiative processes involved.
Aims. In this paper we analyze new observations from the Atacama Large Millimeter/submillimeter Array (ALMA) and the Karl G. Jansky Very Large Array (VLA) at high angular resolution corresponding to 5 – 8 au to determine the dust spatial distribution and grain properties in the ringed disk of HD 163296.
Methods. We fit the spectral energy distribution as a function of the radius at five wavelengths from 0.9 to 9 mm, using a simple power law and a physical model based on an analytic description of radiative transfer that includes isothermal scattering. We considered eight dust populations and compared the models' performance using Bayesian evidence.
Results. Our analysis shows that the moderately high optical depth (τ>1) at λ ≤ 1.3 mm in the dust rings artificially lower the millimeter spectral index, which should therefore not be considered as a reliable direct proxy of the dust properties and especially the grain size. We find that the outer disk is composed of small grains on the order of 200 µm with no significant difference between rings at 66 and 100 au and the adjacent gaps, while in the innermost ~30 au, larger grains (≥mm) could be present. We show that the assumptions on the dust composition have a strong impact on the derived surface densities and grain size. In particular, increasing the porosity of the grains to 80% results in a total dust mass about five times higher with respect to grains with 25% porosity. Finally, we find that the derived opacities as a function of frequency deviate from a simple power law and that grains with a lower porosity seem to better reproduce the observations of HD 163296.
Conclusions. While we do not find evidence of differential trapping in the rings of HD 163296, our overall results are consistent with the postulated presence of giant planets affecting the dust temperature structure and surface density, and possibly originating a second-generation dust population of small grains.