bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRMT6 is Suppressed by miR-191-5p and Functions as a Tumor Promoter in Ovarian Cancer
Authors: Zhao Jing , Wang Runfang , Zhang Yuan , Wang Yazhuo , Li Na , Wang Bei , Zhang Junqin , Han Hua , Zhao Yuan , Xing Hanying , Chu Zhaoping , Tian Fei , Wang Pei , Duan Xiaoyan , Bai Suning , Zhang Yunxia , Zhang Hongzhen 
Published: September 2022

Abstract

Ovarian cancer has a high mortality, and RNA methylation plays a role in several cancers. Our study aimed to explore the effects of differential expression of TRMT6, an N1-methyladenosine writer, on ovarian cancer and its upstream regulatory mechanism. Preliminary bioinformatics analysis revealed that TRMT6 was differentially expressed in ovarian cancer and normal ovarian tissues. Patients with ovarian cancer and high TRMT6 expression had a poor prognosis, and the area under the receiver operating characteristic curve was 0.759. This finding suggests that TRMT6 may be used as a biomarker for ovarian cancer. We used immunohistochemistry to verify the differential expression of TRMT6 in ovarian cancer and normal ovarian tissues. The differential expression of TRMT6 in ovarian cancer cell lines A2780 and OVCAR3 was confirmed using qPCR and western blotting. CCK-8, transwell assay results suggested that overexpression of TRMT6 promoted the proliferation and migration of ovarian cancer cells, whereas overexpression of miR-191-5p reduced these effects. Results of dual-luciferase reporter gene assays indicated that TRMT6 was the target gene of miR-191-5p. In conclusion, TRMT6 promoted the proliferation and migration of ovarian cancer cells, and its upstream miR-191-5p targeted and regulated TRMT6 to reduce these effects. TRMT6 gene expression may be used as a prognostic biomarker and its regulation as a therapeutic target in ovarian cancer.
Enantiomeric resolution of pidotimod and its isomers in pidotimod oral solutions by using HPLC QDa
Authors: Zhang Caiyu , Li Wei , Ning Baoming 
Published: September 2022

Abstract

This study aimed at developing and validating a high-performance liquid chromatography (HPLC) system combined with a quadrupole Dalton mass detector for the separation and determination of pidotimod ((R)-3-[(S)-(5-oxo-2-pyrrolidinyl)carbonyl]-thiazolidine-4-carboxylic acid) and its three isomers. The separations were achieved using a Lux Amylose-1 column operated at 40℃ using acetonitrile/0.1% trifluoroacetate (TFA) and isopropanol/0.1% TFA (90:10, v/v). The enantiorecognition mechanism was also elucidated using the ORCA 5 program. Four isomers were successfully separated with a resolution (Rs) > 1.5, with the method showing satisfactory linearity in the ranges of 0.2 to 3.5 µg·ml− 1(S,R; R,S) and 0.7 to 3.5 µg·ml− 1(S,S; R,R), along with good specificity, accuracy, and precision. Further, three batches of pidotimod oral solutions were evaluated and the S,S-isomer in each batch was detected at about 0.05%. Moreover, computational studies on the sorbent-analyte interactions of amylose tris(3,5-dimethylphenycarbamate) suggested that they can be used to successfully interpret or even predict the chromatographic separation results. The validated method showed high sensitivity, and can potentially be used to analyze the isomer impurities of the raw material for quality control purposes.
Prognostic impact of extended neck dissection in oral squamous cell carcinoma: a retrospective study
Authors: Kugimoto Takuma , Oikawa Yu , Kashima Yoshihisa , Ohsako Toshimitsu , Kuroshima Takeshi , Hirai Hideaki , Tomioka Hirofumi , Harada Hiroyuki 
Published: September 2022

AbstractBackground:Cervical lymph node metastasis with extranodal extension (ENE) is a poor prognostic factor for oral squamous cell carcinoma (OSCC). This study aimed to evaluate the treatment outcomes of OSCC patients who underwent extended neck dissection (ND), requiring resection of non-lymphatic structures for invasive nodal disease.Methods:Patients who underwent extended ND for OSCC at the Department of Oral and Maxillofacial Surgery, Tokyo Medical and Dental University between April 2001 and December 2018 were retrospectively analyzed. Regional recurrence and complication were assessed. The primary outcome variable was 5-year disease-specific survival.Results:A total of 49 patients were included, and regional recurrence was found 18 patients (36.7%). There was no significant difference in the regional recurrence rate, regardless of whether the non-lymphatic structure requiring resection was single or multiple (29.6%vs45.4%,P= 0.398). The regional recurrence rate was significantly higher in patients who required resection of neurovascular structures than in patients who did not require resection of these structures (64.3%vs25.7%,P= 0.028). Cox proportional-hazards regression models indicated that types of resected non-lymphatic structures (with neurovascular structures) was the most risk factor for predicting the risk of regional recurrence (P= 0.048, hazard ratio = 2.59, 95% confidence interval: 1.01–6.66). Cox regression analysis to determine independent predictive factors for DSS, revealing that types of resected non-lymphatic structures (P=0.038, HR = 2.58, 95% CI: 1.05–6.33) was independent predictive factors for DSS. Patients who required resection of neurovascular structures had significantly worse the 5-year DSS rate than those who did not (23.4% vs 63.1%;P= 0.014). Complications after extended ND included recurrent nerve paralysis in three patients, facial nerve paralysis in two patients, and chyle leak, fracture of mandible, and urgent tracheostomy due to larynx edema in a patient, respectively.Conclusion:This study suggests that adhesion of metastatic lymph nodes to neurovascular structures is a significant poor prognostic factor for regional recurrence after extended ND. Patients with OSCC with adhesion of metastatic lymph nodes to neurovascular structures require an adequate safety resection margin and intensive postoperative treatment.
Effects of Drying and Roasting to Effectively use a Discarded Part of Asparagus (Asparagus officinalis L.)
Authors: Kobayashi Fumiyuki , Kimura Ryusuke , Mochizuki Jutaro , Tateishi Naoko , Odake Sachiko 
Published: September 2022

Abstract
Asparagus (Asparagus officinalis L.) has a characteristic flavor and useful components, although the lower stem is not suitable for eating because it has a fibrous skin like wood, being normally discarded. Therefore, to propose drying and roasting as methods for using the hard lower stem of asparagus, usually discarded, useful components in the asparagus stem after drying and roasting were analyzed. The rutin content was decreased significantly by drying and roasting. The ascorbic and folic acids contents were almost unchanged by drying but decreased by roasting. The fructose content was increased by drying, although glucose was almost unchanged. Both were decreased significantly by roasting. The sucrose content was increased by drying but unchanged by roasting. The increase or decrease in some free amino acids were caused after drying, and most of free amino acids disappeared after roasting. Furthermore, characteristic volatile compounds derived from the Maillard reaction were caused by drying and roasting. Most of the useful components in the hard lower stem of asparagus can be concentrated by drying, and characteristic volatile compounds be added by drying and roasting. Therefore, it is promising that the asparagus stem, an unused resource, is able to be utilized as a useful food material by drying and roasting
Global dynamics of Porcine Epidemic Diarrhea Virus evolution and transmission
Authors: Cao Yongchang , Zhang Hao , Zou Chuangchao , Peng Ouyang , Ashraf Usama , Xu Qiuping , Gong Long , Fan Baochao , Zhang Yun , Xu Zhichao , Xue Chunyi , Wei Xiaona , Zhou Qingfeng , Tian Xiaoyan , Shen Hanqin , Zhang Xiangbin , Li Bin 
Published: September 2022

Abstract
With a possible origin from bats, the alphacoronavirus Porcine epidemic diarrhea virus (PEDV) causes significant hazards and widespread epidemics in the swine population. However, the ecology, evolution, and spread of PEDV are still unclear. Here, from 149,869 fecal and intestinal tissue samples of pigs collected in an 11-year survey, we identified PEDV as the most dominant virus in diarrheal animals. Global whole genomic and evolutionary analyses of 672 PEDV strains revealed the fast-evolving PEDV genotype 2 (G2) strains as the main epidemic viruses worldwide, which seems to correlate with the use of G2-targeting vaccines. The evolving pattern of the G2 viruses presents geographic bias as they evolve tachytely in South Korea but undergo the highest recombination in China. Therefore, we clustered six PEDV haplotypes in China, whereas South Korea held five haplotypes, including a unique haplotype G. In addition, an assessment of the spatiotemporal spread route of PEDV indicates Germany and Japan as the primary hubs for PEDV dissemination in Europe and Asia, respectively. Overall, our findings provide novel insights into the epidemiology, evolution, and transmission of PEDV, and thus may lay a foundation for the prevention and control of PEDV and other coronaviruses.
Effect of hydraulic conditions on manganese accumulation by physical and chemical pathways in chlorinated water distribution system: A preliminary laboratory study
Authors: Zhou Xinyi , Nakanishi Tomohiro , Echigo Shinya , Kosaka Koji , Itoh Sadahiko 
Published: September 2022

Abstract
The accumulation of manganese (Mn) in drinking water distribution systems (DWDS) is the main reason for ‘black water’ occurrences at customers' taps. Here, we focused on the impact of hydraulic conditions on Mn accumulation in well chlorinated DWDS, particularly on the physical pathway (i.e., the attachment of particulate Mn to the inner pipe surface) and the chemical pathway (i.e., the autocatalytic oxidation of Mn2+ on the surface of accumulated Mn). Mn accumulation on epoxy resin, a typical lining material for distribution pipes in Japan, was observed in laboratory-scale experiments under different water velocity conditions. The results showed that Mn accumulation was significantly enhanced under higher velocity conditions, which was then fitted with the numerical models describing Mn accumulation by the physical and chemical pathways and the detachment. The rate constants for the physical and chemical pathways had a positive relationship with the flow turbulence in the investigated range, suggesting that hydraulic conditions in DWDS play an important role in Mn accumulation. Effects of Mn speciation and water characteristics on the accumulation process were also simulated under various hydraulic conditions. Based on the obtained results, appropriate countermeasures to control manganese accumulation were then discussed.
Crítica al tratamiento de los créditos por deudas de alimentos o compensación económica en el procedimiento concursal de la persona deudora
Authors: Alarcón Cañuta Miguel Ángel 
Published: September 2022

La consagración de la institución de las personas relacionadas con el deudor persona natural concursada tiene importantes efectosque repercuten de manera significativa en los intereses de cierto tipo de acreedores, como son aquellos a quienes, siendo beneficiarios de una prestación alimenticia o por concepto de compensación económica, se les adeuda un saldo desde antes del inicio del procedimiento concursal de renegociación o liquidación. El estudio del tratamiento que el derecho concursal actual tiene para con los créditos por alimentos o compensación económica de ciertas personas relacionadas al deudor persona natural, nos permitirá plasmar la inconformidad de esta regulación con el respeto de sus intereses desde el punto de vista del respeto de ciertos principios del derecho de familia, manifestada en un trato desfavorable y en muchos casos perjudicial.
Tuberculosis Family Support Training’s (TB FaST) Influence on Encouraging TB Treatment Compliance
Authors: Mariani Helni , Afriandi Irvan , Setiawati Elsa Pudji , Gondodiputro Sharon , Wiwaha Guswan , Nataprawira Heda Melinda , Arisanti Nita , Sumantri Suryana 
Published: September 2022

Background:
Family support is necessary for tuberculosis (TB) treatment compliance. Family intentions and practices in providing support to TB patients require considerable improvement, which is possible via training.

Objective:
This study aimed to analyze the influence of tuberculosis family support training (TB FaST) on the family members’ intentions and practices in encouraging TB treatment compliance in the continuation phase.

Methods:
This quasi-experimental study with a pretest-posttest control group design involved 56 TB patients and 56 family members of patients who had undergone the continuation phase of category 1 TB treatment in the third and fourth months. All patients treated with tuberculosis were directly observed with treatment short-course (TB DOTS) at primary health care. All the subjects were divided into intervention and control groups. TB FaST was given only to the intervention group and delivered using a combination of lectures, case-based focused group discussions, and role-playing in problem-solving over two consecutive days.

Results:
The result showed a significant improvement in the family support practices five weeks after training in the intervention group compared to the control group (p<0.05). However, only a slight improvement was observed in the family intentions (p>0.05).

Conclusion:
TB FaST mainly affects the practices of family members in providing family support to encourage TB treatment compliance in the continuation phase. We recommend that TB FaST or other educational tools be used again after TB FaST to improve intention.
Sodium–Glucose Cotransporter 2 Inhibitors and the Short-term Risk of Bladder Cancer: An International Multisite Cohort Study
Authors: Abrahami Devin , Tesfaye Helen , Yin Hui , Vine Seanna , Hicks Blánaid , Yu Oriana H.Y. , Campeau Lysanne , Platt Robert W. , Schneeweiss Sebastian , Patorno Elisabetta , Azoulay Laurent 
Published: September 2022

OBJECTIVE
To determine whether sodium–glucose cotransporter 2 (SGLT2) inhibitors, compared with glucagon-like peptide 1 receptor agonists (GLP-1RAs) or dipeptidyl peptidase 4 (DPP-4) inhibitors, are associated with an increased risk of early bladder cancer events.

RESEARCH DESIGN AND METHODS
We conducted a multisite, population-based, new-user, active comparator cohort study using the U.K. Clinical Practice Research Datalink, Medicare fee-for-service, Optum’s de-identifed Clinformatics Data Mart Database (CDM), and MarketScan Health databases from January 2013 through December 2020. We assembled two cohorts of adults with type 2 diabetes initiating 1) SGLT2 inhibitors or GLP-1RAs and 2) SGLT2 inhibitors or DPP-4 inhibitors. Cox proportional hazards models were fit to estimate hazard ratios (HRs) and 95% CIs of incident bladder cancer. The models were weighted using propensity score fine stratification. Site-specific HRs were pooled using random-effects models.

RESULTS
SGLT2 inhibitor (n = 453,560) and GLP-1RA (n = 375,997) users had a median follow-up ranging from 1.5 to 2.2 years. Overall, SGLT2 inhibitors were not associated with an increased risk of bladder cancer compared with GLP-1RAs (HR 0.90, 95% CI 0.81–1.00). Similarly, when compared with DPP-4 inhibitors (n = 853,186), SGLT2 inhibitors (n = 347,059) were not associated with an increased risk of bladder cancer (HR 0.99, 95% CI 0.91–1.09) over a median follow-up ranging from 1.6 to 2.6 years. Results were consistent across sensitivity analyses.

CONCLUSIONS
Contrary to previous randomized controlled trials, these findings indicate that the use of SGLT2 inhibitors is not associated with an increased risk of bladder cancer compared with GLP-1RAs or DPP-4 inhibitors. This should provide reassurance on the short-term effects of SGLT2 inhibitors on bladder cancer incidence.
Ordinal Logit Modelling of Socio-Economic Determinants of Tax Evasion Among SMEs in Ghana: Evidence from Sunyani Municipality
Authors: Abdul-Aziz Abdul Rahaman , Abdul Latif Alhassan , Owusu-Ansah Isaac , Agyeiwaa Mary 
Published: September 2022

Taxes consist of direct or indirect taxes and may be paid in money or as its labour equivalent. Taxes are mostly difficult to take from the informal sector since individuals must honour them through self-payment. Due to this, some individuals and cooperate bodies fails to pay voluntarily thereby evading tax. Thus, this paper sought to assess the key determinants of tax evasion and its effect on the Ghanaian economy based on evidence from Sunyani Municipality. The study therefore employed primary source of data collection. Cluster sampling technique was first utilized to group the respondents and subsequently applied systematic random sampling to select the final sample of 250 respondents. The results indicated that nine key determinants of tax evasion were identified. These include waste and corruption by government, complex tax system, high tax burden, compromised tax agents, inadequate tax education, non-consideration of taxpayers’ view, lesser punishment for tax evaders, owners’ unawareness to pay tax and the notion of only the rich pays tax. However, seven of these tax evasion determinants were significantly affecting or influencing the Ghanaian economy whereas the remaining two determinants of tax evasion were found not to contribute significantly, in terms of effect, on the economy. Also, the finding shows that tax evasion does not really depend on the type of business engaged in by SME owners.
Impact of Human Activities on the Carbon Monoxide Levels in Port Harcourt Metropolis: A Rare Experience with Covid-19 Lockdown
Authors: Asimea Sowaboma N. , Fekarurhobo Godswill K. , Gobo Akuro E. , Ndukwe Gloria I. , Inengite Azibaola K. 
Published: September 2022

This study was conducted to monitor the impact of human activities on the air quality of Port Harcourt Metropolis, during and after the Covid-19 Lockdown period where human activities were brought to an all-time low. Ten selected locations, evenly distributed around Port Harcourt Metropolis were studied while Rivers State University served as the Control Site. The meteorological parameters studied alongside carbon monoxide were atmospheric temperature, relative humidity, wind speed, and wind direction. Standard methods were applied in capturing data in-situ, using hand-held devices. Data were collected for six months capturing rainy and dry season months and the lockdown periods. Air Quality Indices were also calculated for CO. The results obtained reveal that meteorological data gathered were characteristic of the study area. The values of carbon monoxide were higher in the Sampling Sites with a mean range of (0.632 – 3.291) ppm in the rainy season and (0.422 – 9.037) ppm in the dry season) than in the Control Site with a mean of (0.133 ppm in the rainy season and 0.493 ppm in the dry season). The level of CO was higher in the dry season as compared to the rainy season. There was strong positive correlation with respect to carbon monoxide amongst sampling locations with similar human activities. The correlation of the sampling locations with the Control Site were mostly weak. A reduction of the concentrations of carbon monoxide was recorded during the Covid-19 Lockdown periods. The AQI values indicate that the air quality was categorized as good with respect to carbon monoxide in the lockdown periods and was between moderate to unhealthy in the non-lockdown periods. It is clearly evident that air quality is a function of the level of human activities, especially those that result in the emission of noxious air pollutants. It is recommended that the air quality be put under regular monitoring in order to regulate the human activities that can impact on the air quality.
UPAYA MENINGKATKAN AKTIVITAS BELAJAR SISWA DENGAN MODEL PEMBELAJARAN KOOPERATIF TIPE STAD (STUDENT TEAM ACHIVEMENT DIVISION) PADA MATA PELAJARAN FISIKA KELAS XI IPA SKM 2 SMA NEGERI 2 PAYAKUMBUH
Authors: Satriadi Erwin 
Published: September 2022

The aim of this classroom action research is to increasen students learning activities and students learning achievement on physics. This research was done in two rounds. The research subject was the students from XI SKM 2 class, SMAN 2 Payakumbuh. The data in this study was collected by using questionare about students learning process activities and test. Data were analyzed by using quantitative qualitative technique. The result showed that: 1) Students ability in oral activities, motor activities, mental activities and emotional activities is still in the average category 2) The increase of student’s visual, listening, writing, and drawing activities happened in a low category 3) The using of cooperative learning model in STAD type can increase students achievements on basic competence.
PENGGUNAAN METODE PEMBELAJARAN MAKE A- MATCH UNTUK MENINGKATKAN PEMAHAMAN BERBELANJA BAHAN MAKANAN SISWA KELAS VIII D di SMP NEGERI 1 TEMPURSARI-LUMAJANG
Authors: Sholihah Binti Robiati 
Published: September 2022

The research aims to know determining the exten to which teaching method of Make A- Match affect students’ level of understanding on how to choose good food materials on grocery shopping chapter. The subjects of the research are grade VIIIth students at Junior High School 1 Tempursari, even semester academic year 2014/2015. This research uses action research with 3 cycles that consists of phases, they are; (1) Action Plan (2) Action Implementation, (3) Observation and (4) Reflection. Learning strategy used is Group Investigation learning method. The data collection uses observation sheet and also competency test result of learning activity or evaluation. Data analyzing technique uses Qualitative Discriptive Technique.
The result of research shows that there is an increasing from the first cycle to the third cycle, that is the average value on beginning tests 65,14 the completenes 51,43%, first cycle the average value of learning result is 71,14 and mastery learning 71,43% the second cycle the average value of learning result reaches 83,72 and learning completeness reaches 82,86% while the third cycle results achieved is very satisfactory with an average value of learning result reaches 93,11 with mastery learning reaches 97,14%. So, it can be concluded that Make A-Match method can increase the student’s learning interest and result.
PENGARUH FASILITAS BELAJAR SISWA, MOTIVASI BERPRESTASI DAN CARA BELAJAR TERHADAP HASIL BELAJAR PADA SISWA KEAHLIAN AKUNTANSI SMK NEGERI KOTA PADANG
Authors: Edriani Devi 
Published: September 2022

This research aims to analyze and see the effect of student learning facilities, achievement motivation and learning styles of the students’ on learning outcomes on student accounting Expertise SMK Padang City. This research is descriptive and associative. The study population all students of Accounting at SMK Padang numbering 822 people, the number of samples 270 people. The sample technique proportionally stratified random sampling. Data and data sources are primary data in the form of a questionnaire filled by the respondents, as well as secondary data about student learning outcomes obtained from documentation. The test instrument validity and reliability. Analyzed using descriptive and inductive analysis, ie test for normality, homogeneity test and test hypotheses using path analysis, F test and t test. The results showed that: 1) Facilities student learning significantly influence student achievement motivation, 2) Facilities student learning and achievement motivation significantly influence to learning styles of syudents, 3) Facilities student learning, achievement motivation and learning styles have a significant and contribution on students’ learning outcomes in Accounting Expertise SMK Padang