bluuebunny/crossref_metadata_2025_split huggingface dataset:
Radiomics in Gastric Cancer: First Clinical Investigation to Predict Lymph Vascular Invasion and Survival Outcome Using 18F-FDG PET/CT Images
Authors: Yang Liping , Chu Wenjie , Li Mengyue , Xu Panpan , Wang Menglu , Peng Mengye , Wang Kezheng , Zhang Lingbo 
Published: March 2022

BackgroundLymph vascular invasion (LVI) is an unfavorable prognostic indicator in gastric cancer (GC). However, there are no reliable clinical techniques for preoperative predictions of LVI. The aim of this study was to develop and validate PET/CT-based radiomics signatures for predicting LVI of GC preoperatively. Radiomics nomograms were also established to predict patient survival outcomes.MethodsThis retrospective study registered 148 GC patients with histopathological confirmation for LVI status, who underwent pre-operative PET/CT scans (Discovery VCT 64 PET/CT system) from December 2014 to June 2019. Clinic-pathological factors (age, gender, and tumor grade, etc.) and metabolic PET data (maximum and mean standardized uptake value, total lesion glycolysis and metabolic tumor volume) were analyzed to identify independent LVI predictors. The dataset was randomly assigned to either the training set or test set in a 7:3 ratios. Three-dimensional (3D) radiomics features were extracted from each PET- and CT-volume of interests (VOI) singularly, and then a radiomics signature (RS) associated with LVI status is built by feature selection. Four models with different modalities (PET-RS: only PET radiomics features; CT-RS: only CT radiomics features; PET/CT-RS: both PET and CT radiomics features; PET/CT-RS plus clinical data) were developed to predict LVI. Patients were postoperatively followed up with PET/CT every 6-12 months for the first two years and then annually up to five years after surgery. The PET/CT radiomics score (Rad-scores) was calculated to assess survival outcome, and corresponding nomograms with radiomics (NWR) or without radiomics (NWOR) were established.ResultsTumor grade and maximum standardized uptake value (SUVmax) were the independent LVI predictor. 1037 CT and PET 3D radiomics features were extracted separately and reduced to 4 and 5 features to build CT-RS and PET-RS, respectively. PET/CT-RS and PET/CT-RS plus clinical data (tumor grade and SUVmax) were also developed. The ROC analysis demonstrated clinical usefulness of PET/CT-RS plus clinical data (AUC values for training and validation, respectively 0.936 and 0.914) and PET/CT-RS (AUC values for training and validation, respectively 0.881 and 0.854), which both are superior to CT-RS (0.838 and 0.824) and PET-RS (0.821 and 0.812). SUVmax and LVI were independent prognostic indicators of both OS and PFS. Decision curve analysis (DCA) demonstrated NWR outperformed NWOR and was established to assess survival outcomes. For estimation of OS and PFS, the C-indexes of the NWR were 0. 88 and 0.88 in the training set, respectively, while the C-indexes of the NWOR were 0. 82 and 0.85 in the training set, respectively.ConclusionsThe PET/CT-based radiomics analysis might serve as a non-invasive approach to predict LVI status in GC patients and provide effective predictors of patient survival outcomes.
Learning Methods During School Closure and Its Correlation With Anxiety and Health Behavior of Thai Students
Authors: Widyastari Dyah Anantalia , Kesaro Sarocha , Rasri Niramon , Saonuam Pairoj , Katewongsa Piyawat 
Published: March 2022

BackgroundThe sequential waves of epidemic spread of COVID-19 in Thailand have caused periodic closures of schools, and exposed students to different learning methods that require multiple adjustment strategies. This study aimed to examine how different learning methods may correlate with anxiety and health behavior (e.g., physical activity, active play, screen time, sleep) of primary and secondary school students in Thailand.MethodsThailand Report Card (TRC) Data (2021) was employed. The sample of the TRC was drawn by multi-stages random sampling stratified by region, district, urban/rural, school size, sex, and age to ensure national representativeness. A total of 6,078 Thai primary (64%) and secondary (36%) school students were included in the analysis.ResultsAbout two-thirds (66.4%) of the sample experienced a shift from traditional classroom to fully online learning, 6.9% experienced partial online instruction, 23.6% received handouts or written assignments, and 3.1% resumed traditional classroom learning. Compared to fully online learning, students who experienced traditional classroom (onsite) teaching were 37.8% less likely to report moderate-to severe anxiety (OR 0.6; p-value 0.021). There was no significant correlation between school closure-induced anxiety with overall physical activity (PA) and active play, but anxiety was significantly associated with screen time and sleep duration. Receiving handouts/written assignments only as the learning method was significantly correlated with PA, but two methods (handouts and onsite/traditional classroom) was significantly correlated with active play. Students who experienced classroom learning were also more likely to comply with recommended durations of screen time and sleep.ConclusionAlthough online learning was probably the most convenient choice during COVID-19 containment measures in Thailand, this method did not provide sufficient opportunity for PA and play. Online learning also encouraged an excessive use of screen media, and disrupted sleeping patterns. Online learning also pressured Thai students to make various adjustments in their daily routines that may have further aggravated anxiety.
The LINCE Project: A Pathway for Diagnosing NCL2 Disease
Authors: Rodrigues Daniel , de Castro Maria José , Crujeiras Pablo , Duat-Rodriguez Anna , Marco Ana Victoria , del Toro Mireia , Couce María L. , Colón Cristóbal 
Published: March 2022

IntroductionNeuronal Ceroid Lipofuscinosis (NCL) comprises a clinically and genetically heterogeneous group of 13 neurodegenerative lysosomal storage disorders. Neuronal Ceroid lipofuscinosis type 2 disease (NCL2), caused by the deficient lysosomal enzyme tripeptidyl peptidase 1 (TPP1), is the only one with an approved enzyme replacement treatment (ERT). Early initiation of ERT appears to modify significantly the natural history of the disease. We aimed to shorten the time to diagnosis of NCL2.MethodsIn March 2017, we started per first time in Spain a selective screening program, the LINCE project, in pediatric patients with clinical symptoms compatible with NCL2 disease. The program covered the whole country. We distributed kits to pediatricians with the necessary material to assess patients. All samples in this study were received within one week of collection. Enzymatic activity determined on dried blood spots was the main method used to screen for TPP1 and palmitoyl protein thioesterase 1 (PPT1) for the differential diagnosis with neuronal ceroid lipofuscinosis type 1 (NCL1).ResultsOver a period of three years, we received 71 samples. The analysis was minimally invasive, relatively cheap and fast-executing. Three cases identified as a direct result of the selective screening strategy were confirmed by genetic study of NCL2 disease with a median age of 4.5 years. Our screening method has a specificity of 100%, and, with the absence to date of false negatives. We did not detect any NCL1-positive cases.ConclusionsLINCE proved to be a simple, useful, and reliable tool for the diagnosis of NCL2, enabling clinicians to diagnose NCL2 faster. The presence of NCL2-positive cases in our population and availability of treatment may facilitate the inclusion of NCL2 in neonatal screening programs for early diagnosis.
In Vitro and In Vivo Studies for the Investigation of γ-Globin Gene Induction by Adhatoda vasica: A Pre-Clinical Study of HbF Inducers for β-Thalassemia
Authors: Iftikhar Fizza , Rahman Saeedur , Khan Muhammad Behroz Naeem , Khan Kanwal , Khan Muhammad Noman , Uddin Reaz , Musharraf Syed Ghulam 
Published: March 2022

Fetal hemoglobin (HbF) is a potent genetic modifier, and the γ-globin gene induction has proven to be a sustainable therapeutic approach for the management of β-thalassemia. In this study, we have evaluated the HbF induction ability of A. vasica in vitro and in vivo, and the identification of potential therapeutic compounds through a bioassay-guided approach. In vitro benzidine-Hb assay demonstrated strong erythroid differentiation of K562 cells by A. vasica extracts. Subsequently, an in vivo study with an aqueous extract of A. vasica (100 mg/kg) showed significant induction of the γ-globin gene and HbF production. While in the acute study, the hematological and biochemical indices were found to be unaltered at the lower dose of A. vasica. Following the bioassay-guided approach, two isolated compounds, vasicinol (1) and vasicine (2) strongly enhanced HbF levels and showed prominent cellular growth kinetics with ample accumulation of total hemoglobin in K562 cultures. High HbF levels were examined by immunofluorescence and flow cytometry analysis, concomitant with the overexpression in the γ-globin gene level. Compound 1 (0.1 µM) and compound 2 (1 µM) resulted in a greater increase in F-cells (90 and 83%) with marked up (8-fold and 5.1-fold) expression of the γ-globin gene, respectively. Molecular docking studies indicated strong binding affinities of (1) and (2) with HDAC2 and KDM1 protein that predict the possible mechanism of compounds in inhibition of these epigenetic regulators in the γ-globin gene reactivation. Altogether, these observations demonstrated the therapeutic usefulness of A. vasica for fostering HbF production in clinical implications for blood disorders.
Hypothermia Induced Impairment of Platelets: Assessment With Multiplate vs. ROTEM—An In Vitro Study
Authors: Wallner Bernd , Schenk Bettina , Paal Peter , Falk Markus , Strapazzon Giacomo , Martini Wenjun Z. , Brugger Hermann , Fries Dietmar 
Published: March 2022

Introduction: This experimental in vitro study aimed to identify and characterize hypothermia-associated coagulopathy and to compare changes in mild to severe hypothermia with the quantitative measurement of rotational thromboelastometry (ROTEM) and multiple-electrode aggregometry (MULTIPLATE).Methods: Whole blood samples from 18 healthy volunteers were analyzed at the target temperatures of 37, 32, 24, 18, and 13.7°C with ROTEM (ExTEM, InTEM and FibTEM) and MULTIPLATE using the arachidonic acid 0.5 mM (ASPI), thrombin receptor-activating peptide-6 32 µM (TRAP) and adenosine diphosphate 6.4 µM (ADP) tests at the corresponding incubating temperatures for coagulation assessment.Results: Compared to baseline (37°C) values ROTEM measurements of clotting time (CT) was prolonged by 98% (at 18°C), clot formation time (CFT) was prolonged by 205% and the alpha angle dropped to 76% at 13.7°C (p &lt; 0.001). At 24.0°C CT was prolonged by 56% and CFT by 53%. Maximum clot firmness was only slightly reduced by ≤2% at 13.7°C. Platelet function measured by MULTIPLATE was reduced with decreasing temperature (p &lt; 0.001): AUC at 13.7°C −96% (ADP), −92% (ASPI) and −91% (TRAP).Conclusion: Hypothermia impairs coagulation by prolonging coagulation clotting time and by decreasing the velocity of clot formation in ROTEM measurements. MULTIPLATE testing confirms a linear decrease in platelet function with decreasing temperatures, but ROTEM fails to adequately detect hypothermia induced impairment of platelets.
A Study of Family Process Factors of Social Anxiety on the Internet Based on Big Data—Take Guangxi University Students as an Example
Authors: Bi Dexu 
Published: March 2022

BackgroundAlong with the popularization of the new medium of interpersonal communication, many researchers have found that the use of social media has brought about many mental health problems. For example, the virtual nature, vulnerability, and uncertainty of online communication lead to reduced online trust, causing interaction anxiety (IA). The data footprints left on the Internet are processed by malicious elements for big data, leading to the leakage of personal privacy data, bringing content sharing anxiety (SAC) and privacy concern anxiety (PAC), which are all typical forms of online social anxiety. In the face of this situation, analyzing the influence of online social networking on the social psychology of university students and guiding it has become an inevitable issue in the Internet era.MethodsLearning from the classification of family environment, a self-administered family process factor questionnaire and the Social Anxiety Scale for Social Media Users (SAS-SMU) were used to investigate the online social anxiety of Guangxi University students. The study used SPSS26.0 and Stata for data analysis and descriptive statistics, ANOVA, t-test, and linear regression analysis were used to explore the relationship between family process factors and online social anxiety of the university students.ResultsThe results showed that except for parental supervision (p &gt; 0.05), the effects of interparental relationship, parent-child relationship, sibling relationship, and family atmosphere on university students' online social anxiety were statistically significant and showed positive correlations (F/t = 6.64, 3.53, 4.15, 5.94; p &lt; 0.05). Multiple linear regression analysis showed that university students' total online social anxiety score = 36.914−4.09 × good parental relationship−4.16 × good family atmosphere−3.42 × good sibling relationship.ConclusionsBased on the family systems theory, it is suggested that a comprehensive intervention should be conducted for the coupled system (parental relationship) and sibling system (non-only child's sibling relationship) in the family and focus on the protective factors of parental harmony, sibling relationship harmony, and relaxed family atmosphere. In the specific implementation method, the collaborative shared healthcare plan (CSHCP) can be used to strengthen remote family emotional interaction and avoid Internet addiction. For university students with online social anxiety disorders, their personal health records (PHRs) can be maintained permanently and safely using the Star File System (IPFS), in addition to the convenience of IPFS data extraction, which is more conducive to the timely and long-term tracking treatment of anxious university students.
Mechanically Reinforced Silkworm Silk Fiber by Hot Stretching
Authors: Lu Haojie , Xia Kailun , Jian Muqiang , Liang Xiaoping , Yin Zhe , Zhang Mingchao , Wang Huimin , Wang Haomin , Li Shuo , Zhang Yingying 
Published: March 2022

Silkworm silk, which is obtained from domesticated
Bombyx mori
(
B. mori
), can be produced in a large scale. However, the mechanical properties of silkworm silk are inferior to its counterpart, spider dragline silk. Therefore, researchers are continuously exploring approaches to reinforce silkworm silk. Herein, we report a facile and scalable hot stretching process to reinforce natural silk fibers obtained from silkworm cocoons. Experimental results show that the obtained hot-stretched silk fibers (HSSFs) retain the chemical components of the original silk fibers while being endowed with increased
β
-sheet nanocrystal content and crystalline orientation, leading to enhanced mechanical properties. Significantly, the average modulus of the HSSFs reaches

21.6
±
2.8

GPa, which is about twice that of pristine silkworm silk fibers (

11.0
±
1.7

GPa). Besides, the tensile strength of the HSSFs reaches

0.77
±
0.13

GPa, which is also obviously higher than that of the pristine silk (

0.56
±
0.08

GPa). The results show that the hot stretching treatment is effective and efficient for producing superstiff, strong, and tough silkworm silk fibers. We anticipate this approach may be also effective for reinforcing other natural or artificial polymer fibers or films containing abundant hydrogen bonds.
Minimally Invasive Epicardial Ablation in Atrial Fibrillation: Evolution of Methods
Authors: Жигалкович А.С. 
Published: March 2022

В статье освещена история развития мини-инвазивной эпикардиальной абляции при изолированной форме фибрилляции предсердий. Неоптимальные результаты использования антиаритмических препаратов и антикоагулянтов, невысокая эффективность катетерной абляции при непароксизмальных формах фибрилляции предсердий, травматичность операции Maze на открытом сердце с применением искусственного кровообращения привели к развитию торакоскопической эпикардиальной абляции. Первоначально основанная на изоляции только легочных вен эпикардиальная абляция со временем перешла в формат фрагментации левого предсердия, максимально повторяя операцию Maze. Эффективность торакоскопической эпикардиальной абляции значимо превосходит таковую при катетерной эндокардиальной абляции при непароксизмальных формах фибрилляции предсердий. Появление новых хирургических методов лечения фибрилляции предсердий создало основу для мультидисциплинарного подхода в лечении этой сложной аритмии и способствовало развитию гибридных технологий.
In the article, there is described the history of the development of minimally invasive epicardial ablation in isolated atrial fibrillation. Non-optimal results of the use of antiarrhythmic drugs and anticoagulants, low efficiency of catheter ablation in non-paroxysmal forms of atrial fibrillation, invasiveness of Maze open heart surgery using heart bypass resulted in the development of thoracoscopic epicardial ablation. Epicardial ablation, which was based initially only on isolation of the pulmonary veins, eventually developed into the left atrial fragmentation format, copying the Maze operation as much as possible. The effectiveness of thoracoscopic epicardial ablation significantly exceeds that of catheter endocardial ablation with non-paroxysmal forms of atrial fibrillation. The use of new surgical methods of treatment of atrial fibrillation created the base for multidisciplinary approach in the treatment of this complex arrhythmia and promoted the development of hybrid technologies.
EL APORTE DE LAS FUENTES HISTÓRICAS PARA EL ANÁLISIS BIOARQUEOLÓGICO Y DE PRÁCTICAS FUNERARIAS DURANTE EL SIGLO XIX EN EL MONUMENTO HISTÓRICO DE SAN JOSÉ DEL MONTE DE LOS LULES (TUCUMÁN – ARGENTINA)
Authors: Taddei Tamara Taddei 
Published: March 2022

La presente investigación se basa en la revalorización de una colección humana hallada en el sitio San José del monte de los Lules (Tucumán, Argentina), que quedóbajo el resguardo del Instituto de Arqueología y Museodesde el año 2001 hasta la actualidad. La puesta en valor de la misma permitió, por un lado, asignar los individuos al periodo republicano (siglo XIX-XX) y, por otro, aportar conocimientos sobre las prácticas sociales, estado sanitario y estilo de vida de las personas inhumadas en el sitio.Asimismo, se mejoraron las condiciones de embalaje de los restos humanos bajo estudio mediante la implementación de un protocolo de conservación preventiva que aboga por su manejo ético,junto con, la generación de un contexto protector. Para ello se parte de una triple aproximación que articula los estudios bioarqueológicos, las prácticas funerarias y los análisis históricos.
La muestra analizada está representada por cinco individuos, dos de ellos de sexo indeterminado, dosindividuos femeninos y uno masculino,que abarcan un rango etario amplio(desde infanteshasta adultos mayores). Los mismosexhiben señales de enfermedades metabólicas, infecciosas y funcionales.
Subarachnoid hemorrhage due to rupture of very small aneurysms of the anterior part of the circle of Willis
Authors: Shnyakin P. G. , Usatova I. S. , Trubkin A. V. , Kazadaeva I. A. 
Published: March 2022

Objective: To assess the frequency of ruptures of very small cerebral aneurysms, features of the perioperative period and outcomes, in comparison with the rupture of ordinarily sized aneurysms.Material and methods: A comparative analysis of the group of patients with ruptured cerebral miliary aneurysms (n = 18) and the group of patients with ruptured cerebral aneurysms of regular size (n = 308) was carried out. All patients underwent open surgery in the first 3 days after the rupture (osteoplastic craniotomy, microsurgical aneurysm clipping). We compared gender, age of patients, severity of the patient’s condition at the moment of admission, severity of subarachnoid hemorrhage, location of aneurysms, aspect ratio, duration of the operation, frequency of intraoperative ruptures, postoperative mortality.Results: It was found that miliary aneurysm rupture occurs in 5.5% of all patients with cerebral aneurysm rupture. The most common cases of rupture of very small aneurysms were in women (77.7%), with a mean age of 50.8 years. Aneurysms of the anterior communicating artery (66.6%) with a narrow neck (average aspect ratio – 2.1) were the most common. Patients with rupture of very small aneurysms were 7.9% more likely to be admitted in a state of subcompensation or decompensation (Hunt-Hess IV–V), they had massive subarachnoid hemorrhage (Fisher III) 19.6% more often than with ruptured aneurysms of regular size. On average, operations in cases of very small aneurysms lasted 30 minutes less than clipping of ordinary aneurysms, but were complicated by intraoperative rupture twice as often (38.8% and 16.5%, respectively). Postoperative mortality in the group of patients with ruptured miliary aneurysms was 5.7% higher than in patients with ruptured aneurysms of regular size.Conclusion: Rupture of cerebral miliary aneurysms is relatively rare. Women of 50–60 years old with very small aneurysms of the anterior communicating artery with a narrow neck constitute the main group of such patients. Massive subarachnoid hemorrhage and severe condition of patients on admission are more common with miliary aneurysms than with ordinarily sized aneurysms. The small size of the aneurysm and the work near the rupture determine the more frequent contact intraoperative rupture when the neck is exposed as compared to operations on larger aneurysms, which negatively affects the treatment outcomes in this group of patients.