bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk Factors for Weak Antibody Response of SARS-CoV-2 Vaccine in Adult Solid Organ Transplant Recipients: A Systemic Review and Meta-Analysis
Authors: Zong Kezhen , Peng Dadi , Yang Hang , Huang Zuotian , Luo Yunhai , Wang Yihua , Xiang Song , Li Tingting , Mou Tong , Wu Zhongjun 
Published: June 2022

ObjectiveThis is the first systematic review and meta-analysis to determine the factors that contribute to poor antibody response in organ transplant recipients after receiving the 2-dose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine.MethodData was obtained from Embase, PubMed, Web of Science, Cochrane Library, China National Knowledge Infrastructure (CNKI), and Chinese Biomedical Literature Database (CBM). Studies reporting factors associated with antibody responses to the 2-dose SARS-CoV-2 vaccine in solid organ transplant recipients were included in our study based on the inclusion and exclusion criteria. Two researchers completed the literature search, screening, and data extraction. Randomized models were used to obtain results. Egger’s test was performed to determine publication bias. Sensitivity analysis was performed to determine the stability of the result. The heterogeneity was determined using the Galbraith plot and subgroup analysis.ResultsA total of 29 studies were included in the present study. The factors included living donor, BNT162b2, tacrolimus, cyclosporine, antimetabolite, mycophenolic acid (MPA) or mycophenolate mofetil (MMF), azathioprine, corticosteroids, high-dose corticosteroids, belatacept, mammalian target of rapamycin (mTOR) inhibitor, tritherapy, age, estimated glomerular filtration rate (eGFR), hemoglobin, and tacrolimus level were significantly different. Multivariate analysis showed significant differences in age, diabetes mellitus, MPA or MMF, high-dose corticosteroids, tritherapy, and eGFR.ConclusionThe possible independent risk factors for negative antibody response in patients with organ transplants who received the 2-dose SARS-CoV-2 vaccine include age, diabetes mellitus, low eGFR, MPA or MMF, high-dose corticosteroids, and triple immunosuppression therapy. mTOR inhibitor can be a protective factor against weak antibody response.Systematic Review RegistrationPROSPERO, identifier CRD42021257965.
RG100204, A Novel Aquaporin-9 Inhibitor, Reduces Septic Cardiomyopathy and Multiple Organ Failure in Murine Sepsis
Authors: Mohammad Shireen , O’Riordan Caroline E. , Verra Chiara , Aimaretti Eleonora , Alves Gustavo Ferreira , Dreisch Klaus , Evenäs Johan , Gena Patrizia , Tesse Angela , Rützler Michael , Collino Massimo , Calamita Giuseppe , Thiemermann Christoph 
Published: June 2022

Sepsis is caused by systemic infection and is a major health concern as it is the primary cause of death from infection. It is the leading cause of mortality worldwide and there are no specific effective treatments for sepsis. Gene deletion of the neutral solute channel Aquaporin 9 (AQP9) normalizes oxidative stress and improves survival in a bacterial endotoxin induced mouse model of sepsis. In this study we described the initial characterization and effects of a novel small molecule AQP9 inhibitor, RG100204, in a cecal ligation and puncture (CLP) induced model of polymicrobial infection. In vitro, RG100204 blocked mouse AQP9 H2O2 permeability in an ectopic CHO cell expression system and abolished the LPS induced increase in superoxide anion and nitric oxide in FaO hepatoma cells. Pre-treatment of CLP-mice with RG100204 (25 mg/kg p.o. before CLP and then again at 8 h after CLP) attenuated the hypothermia, cardiac dysfunction (systolic and diastolic), renal dysfunction and hepatocellular injury caused by CLP-induced sepsis. Post-treatment of CLP-mice with RG100204 also attenuated the cardiac dysfunction (systolic and diastolic), the renal dysfunction caused by CLP-induced sepsis, but did not significantly reduce the liver injury or hypothermia. The most striking finding was that oral administration of RG100204 as late as 3 h after the onset of polymicrobial sepsis attenuated the cardiac and renal dysfunction caused by severe sepsis. Immunoblot quantification demonstrated that RG100204 reduced activation of the NLRP3 inflammasome pathway. Moreover, myeloperoxidase activity in RG100204 treated lung tissue was reduced. Together these results indicate that AQP9 may be a novel drug target in polymicrobial sepsis.
Transcriptome Analysis and Single-Cell Sequencing Analysis Constructed the Ubiquitination-Related Signature in Glioma and Identified USP4 as a Novel Biomarker
Authors: Tang Qikai , Chen Zhengxin , Xie Jiaheng , Mo Chuangqi , Lu Jiacheng , Zhang Qixiang , Wang Zhangjie , Wu Wei , Wang Huibo 
Published: June 2022

BackgroundGlioma, the most frequent malignant tumor of the neurological system, has a poor prognosis and treatment problems. Glioma’s tumor microenvironment is also little known.MethodsWe downloaded glioma data from the TCGA database. The patients in the TCGA database were split into two groups, one for training and the other for validation. The ubiquitination genes were then evaluated in glioma using COX and Lasso regression to create a ubiquitination-related signature. We assessed the signature’s predictive usefulness and role in the immune microenvironment after it was generated. Finally, in vitro experiment were utilized to check the expression and function of the signature’s key gene, USP4.ResultsThis signature can be used to categorize glioma patients. Glioma patients can be separated into high-risk and low-risk groups in both the training and validation cohorts, with the high-risk group having a significantly worse prognosis (P&lt;0.05). Following further investigation of the immune microenvironment, it was discovered that this risk grouping could serve as a guide for glioma immunotherapy. The activity, invasion and migration capacity, and colony formation ability of U87-MG and LN229 cell lines were drastically reduced after the important gene USP4 in signature was knocked down in cell tests. Overexpression of USP4 in the A172 cell line, on the other hand, greatly improved clonogenesis, activity, invasion and migration.ConclusionsOur research established a foundation for understanding the role of ubiquitination genes in gliomas and identified USP4 as a possible glioma biomarker.
Melatonin Rhythm and Its Relation to Sleep and Circadian Parameters in Children and Adolescents With Autism Spectrum Disorder
Authors: Martinez-Cayuelas Elena , Gavela-Pérez Teresa , Rodrigo-Moreno María , Merino-Andreu Milagros , Vales-Villamarín Claudia , Pérez-Nadador Iris , Garcés Carmen , Soriano-Guillén Leandro 
Published: June 2022

IntroductionSleep problems are prevalent among individuals with autism spectrum disorder (ASD), and a role has been attributed to melatonin in this multifactorial comorbidity.MethodsA cross-sectional study was conducted on 41 autistic children and adolescents (9.9 ± 3.02) and 24 children and adolescents with a normal intellectual function (8.42 ± 2.43) were used as controls. Subjects were matched for sex, body mass index, and pubertal stage, and all were drug-naive. Circadian and sleep parameters were studied using an ambulatory circadian monitoring (ACM) device, and saliva samples were collected around the onset of sleep to determine dim light melatonin onset (DLMO).ResultsPrepubertal individuals with ASD presented later DLMO and an earlier decline in melatonin during adolescence. A relationship was found between melatonin and both sleep and circadian parameters. Participants and controls with later DLMOs were more likely to have delayed sleep onset times. In the ASD group, subjects with the later daytime midpoint of temperature had a later DLMO. Later melatonin peak time and DLMO time were related to lower general motor activity and lower stability of its rhythms.ConclusionThe melatonin secretion pattern was different in individuals with ASD, and it showed a relationship with sleep and circadian parameters. Alterations in DLMO have not been previously reported in ASD with the exception of more variable DLMO timing; however, high variability in the study design and sample characteristics prevents direct comparison. The ACM device enabled the measurement of circadian rhythm, a scarcely described parameter in autistic children. When studied in combination with other measures such as melatonin, ACM can offer further knowledge on sleep problems in ASD.
Early Onset Diffusion Abnormalities in Refractory Headache Disorders
Authors: Santoro Jonathan D. , Moon Peter K. , Han Michelle , McKenna Emily S. , Tong Elizabeth , MacEachern Sarah J. , Forkert Nils D. , Yeom Kristen W. 
Published: June 2022

ObjectiveThis study sought to determine if individuals with medically refractory migraine headache have volume or diffusion abnormalities on neuroimaging compared to neurotypical individuals.BackgroundNeuroimaging biomarkers in headache medicine continue to be limited. Early prediction of medically refractory headache and migraine disorders could result in earlier administration of high efficacy therapeutics.MethodsA single-center, retrospective, case control study was performed. All patients were evaluated clinically between 2014 and 2018. Individuals with medically refractory migraine headache (defined by ICDH-3 criteria) without any other chronic medical diseases were enrolled. Patients had to have failed more than two therapeutics and aura was not exclusionary. The initial MRI study for each patient was reviewed. Multiple brain regions were analyzed for volume and apparent diffusion coefficient values. These were compared to 81 neurotypical control patients.ResultsA total of 79 patients with medically refractory migraine headache were included and compared to 74 neurotypical controls without headache disorders. Time between clinical diagnosis and neuroimaging was a median of 24 months (IQR: 12.0–37.0). Comparison of individuals with medically refractory migraine headache to controls revealed statistically significant differences in median apparent diffusion coefficient (ADC) in multiple brain subregions (p &lt; 0.001). Post-hoc pair-wise analysis comparing individuals with medically refractory migraine headache to control patients revealed significantly decreased median ADC values for the thalamus, caudate, putamen, pallidum, amygdala, brainstem, and cerebral white matter. No volumetric differences were observed between groups.ConclusionsIn individuals with medically refractory MH, ADC changes are measurable in multiple brain structures at an early age, prior to the failure of multiple pharmacologic interventions and the diagnosis of medically refractory MH. This data supports the hypothesis that structural connectivity issues may predispose some patients toward more medically refractory pain disorders such as MH.
Use of Teduglutide in Children With Intestinal Failure: A Systematic Review
Authors: Gigola Francesca , Cianci Maria Chiara , Cirocchi Roberto , Ranucci Maria Chiara , Del Riccio Marco , Coletta Riccardo , Morabito Antonino 
Published: June 2022

Background and ObjectivesShort-bowel syndrome (SBS) results from the loss of a significant portion of the small intestine leading to a state of malabsorption. After an intestinal loss, there is a process of adaptation involving the Glucagon-Like Peptide-2 (GLP-2), an enteroendocrine peptide also involved in nutrient absorption. Teduglutide is a recombinant analog of GLP-2 approved in 2016 to treat selected SBS pediatric patients who are dependent on parenteral support. The present systematic review aims to evaluate the efficacy of Teduglutide in pediatric patients with SBS in reducing the need for parenteral nutrition (PN).Materials and MethodsWe performed a literature search on MEDLINE and Embase to include articles up to November 2021. We included articles that involved using Teduglutide in the SBS pediatric population to define its efficacy in reducing the need for PN. The key words used were GLP-2, teduglutide, child.ResultsFourteen studies completely fulfilled the inclusion criteria. Two hundred 23 patients were treated with Teduglutide, and the median duration of treatment was 45 weeks (IQR: 36–52.5 weeks). One-hundred and fifty-two patients were treated with 0.05 mg/Kg/d of subcutaneous Teduglutide, 38 received 0.025 mg/Kg/d and 8 received either 0.125 mg/Kg/d or 0.20 mg/Kg/d. A total of 36 patients achieved enteral autonomy (EA) after a median of 24 weeks of treatment (IQR: 24–48 weeks) and 149 patients showed a reduction in PN needs in terms of volume, calories, or hours per day. Eleven studies reported complications: gastrointestinal were the most common, with 89 cases reported in treated patients and 11 in non-treated patients.ConclusionTeduglutide appears safe and effective in reducing PN requirements and improving EA in the pediatric population. However, more studies are needed to understand its efficacy in the long term and after discontinuation and possible complications.Systematic Review Registration[https://www.crd.york.ac.uk/prospero/], identifier [CRD42022301593].
ERH Interacts With EIF2α and Regulates the EIF2α/ATF4/CHOP Pathway in Bladder Cancer Cells
Authors: Pang Kun , Dong Yang , Hao Lin , Shi Zhen-duo , Zhang Zhi-guo , Chen Bo , Feng Harry , Ma Yu-yang , Xu Hao , Pan Deng , Chen Zhe-sheng , Han Cong-hui 
Published: June 2022

BackgroundThere is a lack of research on the molecular interaction of the enhancers of rudimentary homolog (ERH) in bladder cancer (BC) cells. This study aimed to determine the interacting proteins of ERH in human T24 cells.MethodsFirst, the ERH gene was overexpressed in human T24 cells. Coimmunoprecipitation (co-IP) and shotgun mass spectrometry (MS) analyses were performed to obtain a list of proteins that interact with ERH. Subsequently, bioinformatic analyses with Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein–protein interaction (PPI) studies were performed to analyze the ERH-interactive protein list (ERH-IPL). Then, we selected one of the interacting proteins, EIF2α for verification. An immunofluorescence colocalization assay was performed to validate the co-expression of the selected protein, and the binding sites of the two proteins were predicted by ZDOCK technology. Finally, PCR analysis on the downstream molecules of the interacting protein was performed for verification.ResultsERH protein was successfully overexpressed in human T24 cells. We obtained a list of 205 proteins that might directly or indirectly interact with the ERH protein by mass spectrometric analysis. The bioinformatic analysis showed that ERH-interacting proteins were related to “ribonucleoprotein complex”, “ATPase activity”, “nuclear speck”, and “translation factor activity, RNA binding”. We further identified one of the key genes, EIF2S1, and confirmed that the corresponding protein EIF2α is co-expressed and may bind with ERH in human T24 cells. The mRNA levels of molecules ATF4 and CHOP were found to be upregulated by ERH.ConclusionERH protein affects “ribonucleoprotein complex”, “ATPase activity”, “nuclear speck”, and “translation factor activity, RNA binding”. The ERH protein can interact with EIF2α and regulate the EIF2α-ATF4/CHOP signaling pathway in human T24 cells.
Social and Regional Disparities in Utilization of Maternal and Child Healthcare Services in India: A Study of the Post-National Health Mission Period
Authors: Bango Madhumita , Ghosh Soumitra 
Published: June 2022

BackgroundIndia has enjoyed enhanced economic growth, but has fared poorly in human development indicators and health outcomes, over the last two decades. Significant health inequities and access to healthcare continue to exist and have widened within communities across states. This study examine the changes and disparities in maternal and child healthcare (MCH) among disadvantaged and advanced social groups in three states of India.Data and MethodsFour rounds of National Family Health Survey data were used to measure infant mortality rate (IMR) and under-five mortality rate (U5MR) according to the social groups for the selected states. This study investigates the socio-economic inequities manifested into caste and class differentials and inequities in availability, utilization, and affordability of maternal and healthcare services. Descriptive statistics and the logistic regression model were used. Individual- and household-level covariates were employed to understand the differentials in healthcare utilization.ResultsThe probability of not receiving full antenatal care (ANC) or full immunization for the children was highest among the Scheduled Caste/Scheduled Tribe (SC/ST) families, followed by economic class, mother's education and residence. Tamil Nadu showed the highest utilization of public health facilities, while Bihar was the poorest in terms of health outcomes and utilization of MCH care services even after the pre-National Health Mission (NHM) period. Bihar and West Bengal also showed private healthcare dependence.ConclusionThis study detected the presence of significant caste/tribe differentials in the utilization of MCH care services in the selected states of India. Limited accessibility and unavailability of complete healthcare were the foremost reasons for the under-utilization of these services, especially for people from disadvantaged social groups. The result also suggested that it is perilous to confirm “Health for All” immediately. It will be the efficiency with which India addresses inequities in providing healthcare services and guarantees quality care of health services.
Concerns and Challenges Related to Sputnik V Vaccination Against the Novel COVID-19 Infection in the Russian Federation: The Role of Mental Health, and Personal and Social Issues as Targets for Future Psychosocial Interventions
Authors: Vasileva Anna V. , Karavaeva Tatiana A. , Radionov Dmitriy S. , Yakovlev Alexander V. , Mitin Igor N. , Caroppo Emanuele , Barshak Sergey I. , Nazarov Kirill S. 
Published: June 2022

BackgroundVaccine hesitancy causes serious difficulties in vaccination campaigns in many countries. The study of the population’s attitude toward vaccination and detection of the predictive important individual psychological and social factors defining the vaccination necessity perception will allow elaborating promoting vaccination adherence measures.ObjectivesThe aim of this research was to study COVID-19 threat appraisal, fear of COVID-19, trust in COVID-19 information sources, COVID-19 conspiracy beliefs, and the relationship of sociodemographic variables to COVID-19 preventive behavior.MethodsWe carried out a cohort cross-sectional study of the population’s attitude toward vaccination against the novel COVID-19 coronavirus infection, using a specially designed questionnaire for an online survey. Totally, there were 4,977 respondents, ranging in age from 18 to 81 years. Statistical assessment was carried out using the SPSS-11 program.ResultsThere were different attitudes toward vaccination. Among respondents, 34.2% considered vaccination to be useful, 31.1% doubted its effectiveness, and 9.9% considered vaccination unnecessary. The survey indicated that 7.4% of respondents were indifferent to the vaccine, while 12.2% deemed it to be dangerous. Nearly one-third (32.3%) of respondents indicated that they did not plan to be vaccinated, while another third (34.0%) would postpone their decision until more comprehensive data on the results and effectiveness of vaccination were available. Only 11.6% of the respondents were vaccinated at the time of the study. Young people were less focused on vaccination compared to middle-aged and elderly people. Receiving information concerning COVID-19 vaccination from healthcare workers and scientific experts was associated with greater vaccination acceptance.ConclusionThe study results showed that vaccination attitudes interacted with individuals’ mental health and various sociodemographic factors. Insofar as reports of physicians and experts are essential for shaping attitudes to vaccination, the study results inform the selection of target groups in need of particular psychosocial interventions to overcome their vaccine hesitancy.
Cover Crop Species Affect N2O Emissions at Hotspot Moments of Summer Crops
Authors: Vangeli Sebastian , Restovich Silvina , Posse Gabriela 
Published: June 2022

The use of cover crops, combined with low N fertilization and no-tillage, reduces the environmental impacts of agriculture. Legume cover crops provide N to the agroecosystem and allow N fertilization to be reduced without losing productivity, but may also increase nitrous oxide (N2O) emissions. Our main objective was to evaluate the impact of using oats, vetch, and oats+vetch mixture as cover crops on N2O emissions and summer crop yields in a maize–soybean rotation with low N fertilization to maize (32 kg N ha−1) under no-tillage. We also studied how the different cover crops affected soil variables related to N2O emissions. For the treatments that included vetch (vetch and oats+vetch), plots without N fertilization were included to evaluate if N2O emissions and crop yield were increased by low-rate N fertilization after a legume cover crop. We measured N2O emissions using static chambers in a long-term experiment located in the Argentine Pampas. We selected measurement periods in which high N2O fluxes were expected to evaluate the effect of the different cover crops during these hotspots. In the early stages of maize and soybean, the use of vetch as a cover crop increased N2O emissions compared with oats and a control without a cover crop. In those early stages, conditions for high N2O flux occurrence were promoted by the use of cover crops, as they increased soil moisture and, when vetch was the cover crop, nitrate content. Although the oats+vetch mixture reduced soil nitrate availability compared with vetch, this was not reflected lower N2O emissions. The use of oats as a cover crop did reduce N2O emissions compared with vetch and also decreased maize yields by 30.6%. The low-rate N fertilization in treatments that included vetch as a cover crop did not increase N2O emissions or yield significantly. Our study demonstrates that in low-input cropping systems under no-tillage, the use of legume cover crops can favor yields and also increase N2O emissions during the early stages of the following cash crop. Consequently, future work should explore mitigation strategies during this period.
Case Report: Assessing COVID-19 Transmission in Professional Volleyball in Germany, September to December 2020: An Epidemiological Study
Authors: Morath Oliver , Barsch Friedrich , Lazaro Adhara , Huzly Daniela , Deibert Peter 
Published: June 2022

IntroductionThe SARS-CoV-2 pandemic poses extraordinary challenges in all fields of daily life. The WHO recommended social distancing guidelines and person-to-person contact was strongly discouraged to contain transmission. Team-based sports were questioned and widely debated. However, there is a lack of available evidence on the risk of in-game SARS-CoV-2 transmission. We aim to derive new insights regarding the risk of SARS-CoV2 infection during team sports and provide current opinion on how to behave during training and competition.MethodsWe report on six competitive male volleyball players (national level) of the same team who were infected with COVID-19. The mode of transmission and potential virus spreading within the team was investigated. The entire course of infection was documented by detailed medical history of the players and RT-PCR tests confirmed suspected infections. Serological testing was performed to establish the antibody status of the team.ResultsThe investigation did not show strong evidence of viral transmission within the team during sporting activities. Only two players with PCR-proven infection hat detectable antibodies in two antibody assays.ConclusionPrivate and social gatherings can spread infection into team sports. Clearly defined test strategies and strict adherence to standard COVID-19 hygiene guidelines during sports seasons cannot be overemphasized.