bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kandungan Serat Kasar serta Kecernaan in vitro Ransum Komplit Akibat Penggunaan Daun Bafkenu (Kleinhoviahospita) sebagai Pengganti Rumput Alam
Authors: Kor Ana Ayu , Dami Dato Twenfosel. O , Amalo Daud , M. Kleden Markus 
Published: October 2022

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penggunaan bafkenu (Kleinhovia hospita) dalam ransum komplit sebagai penganti rumput alam terhadap kandungan serat kasar serta kecernaan in vitro bahan kering dan bahan organik. Rancangan yang digunakan adalah rancangan acak lengkap ) dengan 4 perlakuan dan 4 ulangan yaitu R0= Ransum komplit terdiri dari 70% hijauan rumput alam + 30% konsentrat; R1= Ransum komplit terdiri dari 70%hijauan (85% rumput alam + 15% daun bafkenu) + 30% konsentrat; R2= Ransum komplit yang terdiri dari 70%hijauan (70% rumput alam +30% daun bafkenu) + 30% konsentrat; dan R3= Ransum komplit yang terdiri dari 70%hijauan (55% rumput alam + 45% daun bafkenu) + 30% konsentrat. Analisis data menggunakan analysis of variance (ANOVA) dan uji BNT. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh nyata (P<0.05) terhadap kandungan serat kasar , tetapi tidak nyata (P>0,05) terhadap kecernaan in vitro bahan kering serta bahan organik. Pada tingkat substitusi rumput alam dengan daun bafkenu 45% dari proporsi hijauan 70% dalam ransum komplit adalah paling baik dilihat dari bahan kandungan serat kasar meskipun memberikan nilai kecernaan bahan organik dan kecernaan bahan kering yang relatif sama dengan perlakuan lainnya

The purpose of this study was to determine the effect of using bafkenu (Kleinhoviahospita) leaves in complete rations as a substitute for natural grass on crude fiber content and in vitro digestibility of dry matter and organic matter. The design used was a completely randomized design with 4 treatments and 4 replications, namely R0 = complete ration consisting of 70% natural grass forage + 30% concentrate; R1 = Complete ration consisting of 70% forage (85% natural grass + 15% bafkenu leaves) + 30% concentrate; R2 = Complete ration consisting of 70% forage (70% natural grass + 30% bafkenu leaves) + 30% concentrate; and R3 = complete ration consisting of 70% forage (55% natural grass + 45% bafkenu leaves) + 30% concentrate.. The data were analyzed using analysis of variance and if it had a significant effect, it was continued with the BNT test. The results of statistical analysis showed that the treatment had a significant effect (P<0.05) on crude fiber content, was not significant (P>0.05) while the in vitro digestibility of dry matter and organic matter At the level of substitution of natural grass by bafkenu leaves, 45% of the proportion of 70% forage in complete rations was the best in terms of crude fiber content even though it provide the same digestibility of organic matter and dry matter digestibility as other
Kombinasi Tepung Ubi Ungu (Ipomoea Batatas L.) Daun Kelor (Moringa Oleifera) dan Minyak Kelapa sebagai Pengganti Jagung dalam Ransum terhadap Performa dan Mortalitas Puyuh Jantan
Authors: Dapawisi Nofembrianto Erdy , Mulyantini N. G. A. , Ballo Victor  J. 
Published: October 2022

Tujuan penelitian ini untuk mengetahui kombinasi ubi ungu daun kelor dan minyak kelapa sebagai pengganti jagung dalam ransum terhadap konsumsi ransum, pertambahan bobot badan, konversi pakan dan mortalitas puyuh jantan. Metode yang digunakan yaitu rancangan acak kelompok terdiri atas 5 kelompok dan 4 ransum perlakuan yaitu R0: jagung 35,42%, konsentrat 56,43% , dedak 8,15%; R1: 25% kombinasi tepung ubi ungu, daun kelor dan minyak kelapa menggantikan jagung; R2: 50% kombinasi tepung ubi ungu, daun kelor dan minyak kelapa menggantikan jagung; R3: 75% kombinasi tepung ubi ungu, daun kelor dan minyak kelapa menggantikan jagung. Analisis varian membuktikan penggunaan tepung ubi ungu, daun kelor dan minyak kelapa sebagai pengganti jagung tidak berpengaruh nyata (P>0,05) pada konsumsi ransum, pertambahan bobot badan, konversi ransum dan mortalitas puyuh jantan. Dari hasil penelitian disimpulkan bahwa ransum perlakuan tidak memberikan pengaruh terhadap konsumsi ransum, pertambahan bobot badan, konversi ransum dan mortalitas puyuh jantan.

The purpose of this study was to determine the combination of purple sweet potato leaves and coconut oil as a substitute for corn in the ration on ration consumption, body weight gain, feed conversion and mortality of male quail. The method used was a randomized block design consisting of 5 groups and 4 treatment rations, namely R0: corn 35.42%, concentrate 56.43%, bran 8.15%; R1: 25% combination of purple sweet potato flour, Moringa leaves and coconut oil replaces corn; R2: 50% combination of purple sweet potato flour, Moringa leaves and coconut oil replaces corn; R3: 75% combination of purple sweet potato flour, Moringa leaves and coconut oil replaces corn. Analysis of variance proved that the use of purple sweet potato flour, moringa leaves and coconut oil as a substitute for corn had no significant effect (P>0.05) on ration consumption, body weight gain, ration conversion and male quail mortality. From the results of the study, it was concluded that the treatment ration had no effect on ration consumption, body weight gain, ration conversion and mortality of male quail.
Inclusion of Non–English-Speaking Participants in Pediatric Health Research
Authors: Chen Annie , Demaestri Sabrina , Schweiberger Kelsey , Sidani Jaime , Wolynn Riley , Chaves-Gnecco Diego , Hernandez Raquel , Rothenberger Scott , Mickievicz Erin , Cowden John D. , Ragavan Maya I. 
Published: October 2022

ImportanceThe inclusion of non–English-speaking (NES) participants in pediatric research is an essential step to improving health equity for these populations. Although some studies have shown lack of progress in NES research participation in the past decade, few have examined NES inclusivity in pediatric research or details about the practices that researchers have used to communicate with NES participants.ObjectiveTo assess how frequently NES families were included in pediatric research, how rates of inclusion changed over time, what languages were included, and methodological details about oral and written communication with NES participants.Evidence ReviewIn this review, all original investigation articles published in JAMA Pediatrics, Pediatrics, and The Journal of Pediatrics between January 2012 and November 2021 were screened. Eligible articles, which included those based in the US and with human participants, were reviewed to determine whether they included or excluded NES participants or whether or not there was specific mention of language. A second-round review was conducted on the subset of articles that included NES participants to determine methodological details (eg, languages included, type of study, region where the study was located, and oral and written communication practices with NES participants).FindingsOf the 8142 articles screened, 5008 (62%) met inclusion criteria; of these, 469 (9%) included NES participants. The most common language was Spanish (350 [75%]); 145 articles (31%) reported non-English or other language without specification. A total of 230 articles (49%) reported the number of NES participants, and 61 (13%) specified the methods used to determine whether participants preferred a language other than English. In all, 101 (22%) and 136 (29%) articles specified how oral and written communication occurred with NES participants, respectively.Conclusions and RelevanceThis review of 3 pediatric journals provides preliminary evidence suggesting exclusion of NES communities from pediatric research from 2012 to 2021 and highlights an opportunity to provide more methodological detail about communication with NES participants. Best practices for improving inclusivity of NES participants are needed to guide researchers toward improved methods and more relevant results.
The E. coli L‐asparaginase V27T mutant: structural and functional characterization and comparison with theoretical predictions
Authors: Strzelczyk Pawel , Zhang Di , Wlodawer Alexander , Lubkowski Jacek 
Published: October 2022

Bacterial L‐asparaginases have been used for over 40 years as anticancer drugs. Ardalan et al. (Medical Hypotheses 112, 7–17, 2018) proposed that the V27T mutant of Escherichia coli type II L‐asparaginase, EcAII(V27T), should display altered biophysical and catalytic properties compared to the wild‐type enzyme, EcAII(wt), rendering it more favourable as a pharmaceutical. They postulated that EcAII(V27T) would exhibit reduced glutaminolytic activity and be more stable compared to EcAII(wt). Their postulates, however, were purely theoretical. Here, we characterized experimentally selected properties of EcAII(V27T). We found asparaginolytic activity of this mutant unchanged, whereas its glutaminolytic activity was fourfold lower compared with EcAII(wt). We did not observe significant differences in stabilities of EcAII(wt) and EcAII(V27T). Crystal structures of the complexes with L‐Asp and L‐Glu showed considerable differences in binding modes of both substrates.
Designing 3D Polymeric Structures through Capillary Wetting on Colloidal Monolayer
Authors: Lee Hyeong Jun , Park Sanghyuk , Kim Jong Bin , Kim Shin‐Hyun 
Published: October 2022

AbstractColloidal lithography has provided a facile means to create regular micro‐ and nano‐patterns by employing colloidal crystals as masks. However, the patterns are usually restricted to 2D as most techniques do not exploit the spherical shape of colloidal masks. Here, 3D structures are designed by utilizing capillary wetting of liquified photoresists on colloidal monolayers of silica particles. The silica particles are anchored at planar air‐photoresist interfaces to have an equilibrium contact angle without interfacial deformation for thick polymer layers. By contrast, when the polymer layers are thin enough, they wet the colloidal monolayer by forming periodic wavy interfaces to provide a constant Laplace pressure. The photo‐crosslinking of the photoresist and subsequent removal of silica particles leave behind periodic nanostructures with 3D‐undulated surfaces. Importantly, the structure and waviness are further controllable by adjusting the polymer thickness relative to the particle radius. As the shape of the interface is determined by capillarity, the 3D structures are reproducible as long as the dimension is smaller than the capillary length. The use of photoresists enables the production of micropatterns of the periodic wavy structures by photolithography. As one of the potential applications, structurally‐colored patterns are demonstrated with enhanced plasmonic and diffraction colors.
Fully Lignocellulosic Biomass‐Based Double‐Layered Porous Hydrogel for Efficient Solar Steam Generation
Authors: Lin Xuliang , Wang Ping , Hong Ruitong , Zhu Xi , Liu Yingchun , Pan Xuejun , Qiu Xueqing , Qin Yanlin 
Published: October 2022

AbstractSolar‐driven interfacial evaporation is an important approach for solving the issue of freshwater scarcity. However, the practical application of solar steam generation is hindered by high fabrication cost and environmental concerns regarding the petroleum‐based materials. Herein, lignocellulose (cellulose‐lignin composite) hydrogel (LCG) and lignin‐derived carbon (LC) are used as the substrate and photothermal material, respectively, to construct a fully lignocellulose‐based double‐layered hydrogel (LC@LCG) evaporator. Results indicate that LC has an ultrahigh specific surface area and full‐spectrum solar absorption of 98%. The presence of lignin can improve the hydrophilicity and maintain the capillary channels of the hydrogel, which tunes water into an intermediate state and reduces the vaporization enthalpy of water. Moreover, it ensures a high water transport rate in the hydrogel. Based on these advantages, the evaporation rate and photothermal conversion efficiency of hydrogel evaporator reach 1.84 kg m−2 h−1 under one sun and 86.5%, respectively. The lignocellulosic hydrogel evaporator could remove >99.95% of primary metal ions from seawater to generate fresh water, and shows outstanding salt resistance, durability, and long‐term stability for desalination. This study demonstrates an eco‐friendly and economic solution for continuous freshwater production from seawater using a fully lignocellulosic biomass‐based hydrogel evaporator.
Large‐Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors
Authors: Ho Debbie L. L. , Lee Stacey , Du Jianyi , Weiss Jonathan D. , Tam Tony , Sinha Soham , Klinger Danielle , Devine Sean , Hamfeldt Art , Leng Hope T. , Herrmann Jessica E. , He Mengdi , Fradkin Lee G. , Tan Tze Kai , Standish David , Tomasello Peter , Traul Donald , Dianat Noushin , Ladi Rukmini , Vicard Quentin , Katikireddy Kishore , Skylar‐Scott Mark A. 
Published: October 2022

AbstractCombining the sustainable culture of billions of human cells and the bioprinting of wholly cellular bioinks offers a pathway toward organ‐scale tissue engineering. Traditional 2D culture methods are not inherently scalable due to cost, space, and handling constraints. Here, the suspension culture of human induced pluripotent stem cell‐derived aggregates (hAs) is optimized using an automated 250 mL stirred tank bioreactor system. Cell yield, aggregate morphology, and pluripotency marker expression are maintained over three serial passages in two distinct cell lines. Furthermore, it is demonstrated that the same optimized parameters can be scaled to an automated 1 L stirred tank bioreactor system. This 4‐day culture results in a 16.6‐ to 20.4‐fold expansion of cells, generating approximately 4 billion cells per vessel, while maintaining >94% expression of pluripotency markers. The pluripotent aggregates can be subsequently differentiated into derivatives of the three germ layers, including cardiac aggregates, and vascular, cortical and intestinal organoids. Finally, the aggregates are compacted into a wholly cellular bioink for rheological characterization and 3D bioprinting. The printed hAs are subsequently differentiated into neuronal and vascular tissue. This work demonstrates an optimized suspension culture‐to‐3D bioprinting pipeline that enables a sustainable approach to billion cell‐scale organ engineering.
Toxicity and Biocompatibility of Liquid Metals
Authors: Chen Sen , Zhao Ruiqi , Sun Xuyang , Wang Hongzhang , Li Lei , Liu Jing 
Published: October 2022

AbstractRecently, room‐temperature liquid metals have attracted increasing attention from researchers owing to their excellent material properties. Systematic interpretation of the potential toxicity issues involved is essential for a wide range of applications, especially in the biomedical and healthcare fields. However, even with the exponential growth of related studies, investigation of the toxicological impact and possible hazards of liquid metals to organisms is still in its infancy. This review aims to provide a comprehensive summary of the current frontier of knowledge on liquid metal toxicology and biocompatibility in different environments. Based on recent studies, this review focuses on Ga and Bi‐based in different states. It is necessary to evaluate their toxicity considering the rapid increase in research and utilization of such liquid metal composites. Finally, existing challenges are discussed and suggestions are provided for further investigation of liquid metal toxicology to clarify the toxicological mechanisms and strategies are provided to avoid adverse effects. In addition to resolving the doubts of public concern about the toxicity of liquid metals, this review is expected to promote the healthy and sustainable development of liquid metal‐based materials and their use in diverse areas, especially those related to health care.
Miniature NIR‐II Nanoprobes for Active‐Targeted Phototheranostics of Brain Tumors
Authors: Wu Yayun , Hu Dehong , Gao Duyang , Liu Chunchen , Zheng Hairong , Sheng Zonghai 
Published: October 2022

AbstractNanoprobes (NPs) in the second near‐infrared biowindow (NIR‐II, 1000–1700 nm) are developed and widely used in cancer phototheranostics. However, most NIR‐II NPs exhibit low phototheranostic efficiency due to their tedious synthetic routes, large particle sizes (>20 nm), and lack of active targeting properties. Here, miniature NIR‐II NPs, named HSA‐ICG‐iRGD, for active‐targeted NIR‐II phototheranostics of brain tumors are reported. The HSA‐ICG‐iRGD probes are designed based on hydrophobic interactions as well as hydrogen bonds between albumin and indocyanine green derivatives (ICG‐iRGD) via molecular docking. The as‐prepared NPs have a compact size of 10 nm and show tumor‐targeting ability by specifically binding to αvβ3 integrin receptors which are highly expressed on the surface of brain tumor cells via iRGD peptides. The HSA‐ICG‐iRGD NPs are then applied to perform active‐targeted NIR‐II fluorescence imaging, resulting in a signal‐to‐background ratio of 6.85 in orthotopic glioma mouse models. Under the selected laser irradiation of 808 nm, the photothermal effect of HSA‐ICG‐iRGD extends the survival of the tumor‐bearing mice to 55 days, significantly longer than that of the control group (30 days). These results highlight the potential of miniature NPs for active‐targeted NIR‐II fluorescence imaging and phototherapy of brain tumors.
Sulfonium‐Cations‐Assisted Intermediate Engineering for Quasi‐2D Perovskite Solar Cells
Authors: Wang Boxin , Cheng Qian , Huang Gaosheng , Yue Yaochang , Zhang Weichuan , Li Xing , Li Yanxun , Du Wenna , Liu Xinfeng , Zhang Hong , Zhang Yuan , Zhou Huiqiong 
Published: October 2022

AbstractQuasi‐2D Ruddlesden–Popper (RP) perovskites with superior stability are admirable candidates for perovskite solar cells (PSCs) toward commercialization. However, the device performance remains unsatisfactory due to the disordered crystallization of perovskites. In this work, the effects of sulfonium cations on the evolution of intermediates and photovoltaic properties of 2D RP perovskites are investigated. The introduction of sulfonium cations leads to preferred intermediate transformation and improved film quality of perovskites. The resulting devices deliver a champion efficiency of 19.08% at room temperature and 20.52% at 180 K, due to reduced recombination and enhanced charge transport. More importantly, the unencapsulated device maintains 84% of the initial efficiency under maximum power point (MPP) tracking at 40 °C for 1000 h. This work helps to gain a comprehensive understanding of the crystallization process of quasi‐2D perovskites and provides a simple strategy to modulate the intermediates of perovskites.
Stretchable Transparent Polyelectrolyte Elastomers for All‐Solid Tunable Lenses of Excellent Stability Based on Electro–Mechano–Optical Coupling
Authors: Zhong Hao , Xue Qiqi , Li Jiameng , He Yunfeng , Xie Yuxin , Yang Canhui 
Published: October 2022

AbstractLenses are ubiquitously used for imaging. Compared with conventional lenses containing rigid translating components driven by mechanical motors, nonmechanical tunable lenses have been drawing attention owing to their advantages in compactness, lightweight, low power consumption, and fast response. Herein, inspired by the accommodation mechanism of the human eye, an all‐solid electromechanically tunable lens driven by a dielectric elastomer actuator is presented. A soft, stretchable, transparent, and ionically conductive polyelectrolyte elastomer, poly(3‐acrylamidopropyl)trimethylammonium chloride, is synthesized and used as the electrodes, one of which is plano‐convex and the other planar. A mold‐free procedure is elaborated to eliminate the contamination by molding and for facile fabrication. Subject to voltage, the resulting tunable lens achieves a relative change of focal length of ≈46.4%, superior to that of the human eye. The electro–mechano–optical coupling of the lens is modeled and the theoretical predictions agree well with the experimental results. Moreover, the tunable lens responds fast, operates stably in the ambient and desiccated environment, maintains performances over 1000 cycles, and exhibits a shelf‐life longer than 12 weeks. The polyelectrolyte elastomer‐based all‐solid tunable lens promises a potential solution for lightweight, compact, and durable imaging systems.
Kirigami Design of Flexible and Conformal Tactile Sensor on Sphere‐shaped Surface for Contact Force Sensing
Authors: Wang Shihang , Wang Yancheng , Chen Zhijian , Mei Deqing 
Published: October 2022

AbstractFlexible tactile sensors can be used as the human–machine interactions for tactile information. Integration of flexible tactile sensor onto complex curved surfaces usually generates high assembly strains or stresses, thus reducing the accuracy of tactile sensing. This paper presents a novel flexible tactile sensor using a devisable kirigami structural design for conformal integration of curved surfaces with low assembly strain. The target sphere object's surface is divided into several fan‐shaped pieces and then flattened into 2D patterns for the structural design and arrangement of tactile sensors. Each piece of tactile sensor has three sensing units including one triangular‐shaped and two quadrilateral‐shaped sensing units. The fabricated tactile sensor with tri‐unit and quad‐unit is characterized with the sensitivity of 0.0112 and 0.0081 kPa−1 in sensing range of 0–80 kPa, respectively. Numerical simulation results show the tactile sensor has generally low assembly strain (<1.0%) when integrated onto a tennis ball, and experimental results demonstrate that the relative changes of initial resistance and calculated sensitivity are less than 4.0% before and after integration. The contact force sensing in tennis ball games with different actions demonstrates the potential of using the designed tactile sensor for further athletic training and other applications.
Core‐Shelled Nanoparticle Fillers for Recoverable Magnetic Liquid Metal with High Stability
Authors: Huang Mingdeng , Lin Weiguang , Tuersun Yisimayili , Huang Xu , Chu Sheng 
Published: October 2022

AbstractRoom‐temperature liquid metals, such as galinstan, have become an emerging material in electronics requiring stretchability and deformability due to its excellent fluidity and conductivity. Eutectic gallium‐indium‐tin (galinstan)‐based composites with certain metallic fillers can obtain magnetism. However, the alloying reaction between galinstan and metals leads to the solidification and reduction in stability of the composite. In this study, Ni@SiO2 nanoparticles with the core–shell structure are used as fillers and galinstan as matrix. The obtained galinstan‐Ni@SiO2 paste is fairly stable and this is attributed to the silica shell, which acts as a barrier to isolate the direct contact between the Ni and galinstan. In addition, flexible circuits composed of as‐prepared galinstan‐Ni@SiO2 pastes are fabricated, which exhibited outstanding durability, retrievability, and self‐healing ability under the drive of magnetic field.