bluuebunny/crossref_metadata_2025_split huggingface dataset:
High antibody levels and reduced cellular response in children up to one year after SARS-CoV-2 infection
Authors: Jacobsen Eva-Maria , Fabricius Dorit , Class Magdalena , Topfstedt Fernando , Lorenzetti Raquel , Janowska Iga , Schmidt Franziska , Staniek Julian , Zernickel Maria , Stamminger Thomas , Dietz Andrea N. , Zellmer Angela , Hecht Manuel , Rauch Peter , Blum Carmen , Ludwig Carolin , Jahrsdörfer Bernd , Schrezenmeier Hubert , Heeg Maximilian , Mayer Benjamin , Seidel Alina , Groß Rüdiger , Münch Jan , Kirchhoff Frank , Bode Sebastian F. N. , Strauss Gudrun , Renk Hanna , Elling Roland , Stich Maximillian , Voll Reinhard E. , Tönshof Burkhard , Franz Axel R. , Henneke Philipp , Debatin Klaus-Michael , Rizzi Marta , Janda Ales 
Published: November 2022

AbstractThe COVID-19 course and immunity differ in children and adults. We analyzed immune response dynamics in 28 families up to 12 months after mild or asymptomatic infection. Unlike adults, the initial response is plasmablast-driven in children. Four months after infection, children show an enhanced specific antibody response and lower but detectable spike 1 protein (S1)-specific B and T cell responses than their parents. While specific antibodies decline, neutralizing antibody activity and breadth increase in both groups. The frequencies of S1-specific B and T cell responses remain stable. However, in children, one year after infection, an increase in the S1-specific IgA class switch and the expression of CD27 on S1-specific B cells and T cell maturation are observed. These results, together with the enhanced neutralizing potential and breadth of the specific antibodies, suggest a progressive maturation of the S1-specific immune response. Hence, the immune response in children persists over 12 months but dynamically changes in quality, with progressive neutralizing, breadth, and memory maturation. This implies a benefit for booster vaccination in children to consolidate memory formation.
Paired electrolysis-enabled nickel-catalyzed enantioselective reductive cross-coupling between α-chloroesters and aryl bromides
Authors: Liu Dong , Liu Zhao-Ran , Wang Zhen-Hua , Ma Cong , Herbert Simon , Schirok Hartmut , Mei Tian-Sheng 
Published: November 2022

AbstractElectrochemical asymmetric catalysis has emerged as a sustainable and promising approach to the production of chiral compounds and the utilization of both the anode and cathode as working electrodes would provide a unique approach for organic synthesis. However, precise matching of the rate and electric potential of anodic oxidation and cathodic reduction make such idealized electrolysis difficult to achieve. Herein, asymmetric cross-coupling between α-chloroesters and aryl bromides is probed as a model reaction, wherein alkyl radicals are generated from the α-chloroesters through a sequential oxidative electron transfer process at the anode, while the nickel catalyst is reduced to a lower oxidation state at the cathode. Radical clock studies, cyclic voltammetry analysis, and electron paramagnetic resonance experiments support the synergistic involvement of anodic and cathodic redox events. This electrolytic method provides an alternative avenue for asymmetric catalysis that could find significant utility in organic synthesis.
Limited carbon cycling due to high-pressure effects on the deep-sea microbiome
Authors: Amano Chie , Zhao Zihao , Sintes Eva , Reinthaler Thomas , Stefanschitz Julia , Kisadur Murat , Utsumi Motoo , Herndl Gerhard J. 
Published: November 2022

AbstractDeep-sea microbial communities are exposed to high-pressure conditions, which has a variable impact on prokaryotes depending on whether they are piezophilic (that is, pressure-loving), piezotolerant or piezosensitive. While it has been suggested that elevated pressures lead to higher community-level metabolic rates, the response of these deep-sea microbial communities to the high-pressure conditions of the deep sea is poorly understood. Based on microbial activity measurements in the major oceanic basins using an in situ microbial incubator, we show that the bulk heterotrophic activity of prokaryotic communities becomes increasingly inhibited at higher hydrostatic pressure. At 4,000 m depth, the bulk heterotrophic prokaryotic activity under in situ hydrostatic pressure was about one-third of that measured in the same community at atmospheric pressure conditions. In the bathypelagic zone—between 1,000 and 4,000 m depth—~85% of the prokaryotic community was piezotolerant and ~5% of the prokaryotic community was piezophilic. Despite piezosensitive-like prokaryotes comprising only ~10% (mainly members of Bacteroidetes,Alteromonas) of the deep-sea prokaryotic community, the more than 100-fold metabolic activity increase of these piezosensitive prokaryotes upon depressurization leads to high apparent bulk metabolic activity. Overall, the heterotrophic prokaryotic activity in the deep sea is likely to be substantially lower than hitherto assumed, with major impacts on the oceanic carbon cycling.
Feedback-controlled hydrogels with homeostatic oscillations and dissipative signal transduction
Authors: Zhang Hang , Zeng Hao , Eklund Amanda , Guo Hongshuang , Priimagi Arri , Ikkala Olli 
Published: November 2022

AbstractDriving systems out of equilibrium under feedback control is characteristic for living systems, where homeostasis and dissipative signal transduction facilitate complex responses. This feature not only inspires dissipative dynamic functionalities in synthetic systems but also poses great challenges in designing novel pathways. Here we report feedback-controlled systems comprising two coupled hydrogels driven by constant light, where the system can be tuned to undergo stable homeostatic self-oscillations or damped steady states of temperature. We demonstrate that stable temperature oscillations can be utilized for dynamic colours and cargo transport, whereas damped steady states enable signal transduction pathways. Here mechanical triggers cause temperature changes that lead to responses such as bending motions inspired by the single-touch mechanoresponse in Mimosa pudica and the frequency-gated snapping motion inspired by the plant arithmetic in the Venus flytrap. The proposed concepts suggest generalizable feedback pathways for dissipative dynamic materials and interactive soft robotics.
Distinct changes in endosomal composition promote NLRP3 inflammasome activation
Authors: Zhang Zhirong , Venditti Rossella , Ran Li , Liu Zengzhen , Vivot Karl , Schürmann Annette , Bonifacino Juan S. , De Matteis Maria Antonietta , Ricci Romeo 
Published: November 2022

AbstractInflammasome complexes are pivotal in the innate immune response. The NLR family pyrin domain containing protein 3 (NLRP3) inflammasome is activated in response to a broad variety of cellular stressors. However, a primary and converging sensing mechanism by the NLRP3 receptor initiating inflammasome assembly remains ill defined. Here, we demonstrate that NLRP3 inflammasome activators primarily converge on disruption of endoplasmic reticulum–endosome membrane contact sites (EECS). This defect causes endosomal accumulation of phosphatidylinositol 4-phosphate (PI4P) and a consequent impairment of endosome-to-trans-Golgi network trafficking (ETT), necessary steps for endosomal recruitment of NLRP3 and subsequent inflammasome activation. Lowering endosomal PI4P levels prevents endosomal association of NLRP3 and inhibits inflammasome activation. Disruption of EECS or ETT is sufficient to enhance endosomal PI4P levels, to recruit NLRP3 to endosomes and to potentiate NLRP3 inflammasome activation. Mice with defects in ETT in the myeloid compartment are more susceptible to lipopolysaccharide-induced sepsis. Our study thus identifies a distinct cellular mechanism leading to endosomal NLRP3 recruitment and inflammasome activation.
Tribotronic control of an ionic boundary layer in operando extends the limits of lubrication
Authors: Reddy Akepati Bhaskar , Pilkington Georgia A. , Rutland Mark W. , Glavatskih Sergei 
Published: November 2022

AbstractThe effect of electric potential on the lubrication of a non-halogenated phosphonium orthoborate ionic liquid used as an additive in a biodegradable oil was studied. An in-house tribotronic system was built around an instrument designed to measure lubricant film thickness between a rolling steel ball and a rotating silica-coated glass disc. The application of an electric field between the steel ball and a set of customized counter-electrodes clearly induced changes in the thickness of the lubricant film: a marked decrease at negative potentials and an increase at positive potentials. Complementary neutron reflectivity studies demonstrated the intrinsic electroresponsivity of the adsorbate: this was performed on a gold-coated silicon block and made possible in the same lubricant system by deuterating the oil. The results indicate that the anions, acting as anchors for the adsorbed film on the steel surface, are instrumental in the formation of thick and robust lubricating ionic boundary films. The application of a high positive potential, outside the electrochemical window, resulted in an enormous boost to film thickness, implicating the formation of ionic multi-layers and demonstrating the plausibility of remote control of failing contacts in inaccessible machinery, such as offshore wind and wave power installations.
Mechanical properties and acoustic emission analysis of desert sand concrete reinforced with steel fiber
Authors: Qu Changwei , Qin Yongjun , Luo Ling , Zhang Liangliang 
Published: November 2022

AbstractIn this study, to reduce the consumption of natural sand and improve the utilization rate of desert sand in western China, while preparing 14 groups of samples, desert sand is used to replace natural sand by the ratio of 20%, 40%, and 60%, and steel fiber is mixed with volume fraction 0.5%, 1.0%, 1.5%, and 2.0%. The mechanical properties of the specimens, including compressive strength, splitting tensile strength, and axial compressive strength were tested. Besides, the microstructures of the samples were analyzed by SEM, XRD, and acoustic emission detection technologies to identify the damage process. The results show that the desert sand can refine the microstructure and fill the pores, and it has good comprehensive properties at a 40% substitution rate. The compression properties of specimens are not apparently improved, but the tensile strength and deformation properties are significantly improved. The steel fiber with 1.5 vol% content behaves better, and the 28d compressive strength of the optimized group reaches 58.7 MPa. As a result, the polynomial fitting degree of total AE hits and stress level receives a more incredible goodness (R2) value than 0.96. The strength characteristics of steel fiber-desert sand concrete (SFDSC) can meet the demands of C40 concrete, and this research can provide a reference for engineers using desert sand in their designs.
Physics inspired compact modelling of $$\hbox {BiFeO}_3$$ based memristors
Authors: Yarragolla Sahitya , Du Nan , Hemke Torben , Zhao Xianyue , Chen Ziang , Polian Ilia , Mussenbrock Thomas 
Published: November 2022

AbstractWith the advent of the Internet of Things, nanoelectronic devices or memristors have been the subject of significant interest for use as new hardware security primitives. Among the several available memristors, BiFe$$\mathrm{O}_{3}$$

O
3

(BFO)-based electroforming-free memristors have attracted considerable attention due to their excellent properties, such as long retention time, self-rectification, intrinsic stochasticity, and fast switching. They have been actively investigated for use in physical unclonable function (PUF) key storage modules, artificial synapses in neural networks, nonvolatile resistive switches, and reconfigurable logic applications. In this work, we present a physics-inspired 1D compact model of a BFO memristor to understand its implementation for such applications (mainly PUFs) and perform circuit simulations. The resistive switching based on electric field-driven vacancy migration and intrinsic stochastic behaviour of the BFO memristor are modelled using the cloud-in-a-cell scheme. The experimental current–voltage characteristics of the BFO memristor are successfully reproduced. The response of the BFO memristor to changes in electrical properties, environmental properties (such as temperature) and stress are analyzed and consistant with experimental results.
Novice-performed point-of-care ultrasound for home-based imaging
Authors: Duggan Nicole M. , Jowkar Nick , Ma Irene W. Y. , Schulwolf Sara , Selame Lauren A. , Fischetti Chanel E. , Kapur Tina , Goldsmith Andrew J. 
Published: November 2022

AbstractPatient-performed point-of-care ultrasound (POCUS) may be feasible for use in home-based healthcare. We investigated whether novice users can obtain lung ultrasound (LUS) images via self-scanning with similar interpretability and quality as experts. Adult participants with no prior medical or POCUS training, who were capable of viewing PowerPoint slides in their home and who could hold a probe to their chest were recruited. After training, volunteers self-performed 8-zone LUS and saved images using a hand-held POCUS device in their own home. Each 8-zone LUS scan was repeated by POCUS experts. Clips were independently viewed and scored by POCUS experts blinded to performing sonographers. Quality and interpretability scores of novice- and expert-obtained LUS images were compared. Thirty volunteers with average age of 42.8 years (Standard Deviation (SD) 15.8), and average body mass index of 23.7 (SD 3.1) were recruited. Quality of novice and expert scans did not differ (median score 2.6, interquartile range (IQR) 2.3–2.9 vs. 2.8, IQR 2.3–3.0, respectively p = 0.09). Individual zone quality also did not differ (P > 0.05). Interpretability of LUS was similar between expert and novice scanners (median 7 zones interpretable, IQR 6–8, for both groups, p = 0.42). Interpretability of novice-obtained scans did not differ from expert scans (median 7 out of 8 zones, IQR 6–8, p = 0.42). Novice-users can self-obtain interpretable, expert-quality LUS clips with minimal training. Patient-performed LUS may be feasible for outpatient home monitoring.
Perceived safety in human–cobot interaction for fixed-path and real-time motion planning algorithms
Authors: Tusseyeva Inara , Oleinikov Artemiy , Sandygulova Anara , Rubagotti Matteo 
Published: November 2022

AbstractThis study investigates how different motion planning algorithms, implemented on a collaborative robot (cobot), are perceived by 48 human subjects. The four implemented algorithms ensure human safety based on the concept ofspeed and separation monitoring, but differ based on the following characteristics: (a) the cobot motion happens either along a fixed path or with a trajectory that is continuously planned in real time via nonlinear model predictive control, to increase cobot productivity; (b) the cobot speed is further reduced—or not—in real time based on heart rate measurements, to increase perceived safety. We conclude that (1) using a fixed path—compared to real-time motion planning—may reduce productivity and, at least when heart rate measurements are not used to modify the cobot speed, increases perceived safety; (2) reducing cobot speed based on heart rate measurements reduces productivity but does not improve perceived safety; (3) perceived safety is positively affected by habituation during the experiment, and unaffected by previous experience.
Characterization of a new potent and long-lasting single chain peptide agonist of RXFP1 in cells and in vivo translational models
Authors: Illiano Stephane , Poirier Bruno , Minoletti Claire , Pasquier Olivier , Riva Laurence , Chenede Xavier , Menguy Isabelle , Guillotel Michel , Prigent Philippe , Le Claire Stéphane , Gillot Florence , Thill Gilbert , Lo Presti François , Corbier Alain , Le Bail Jean-Christophe , Grailhe Patrick , Monteagudo Edith , Ingenito Raffaele , Bianchi Elisabetta , Philippo Christophe , Duclos Olivier , Mallart Sergio , Bathgate Ross , Janiak Philip 
Published: November 2022

AbstractDespite beneficial effects in acute heart failure, the full therapeutic potential of recombinant relaxin-2 has been hampered by its short half-life and the need for intravenous administration limiting its use to intensive care units. A multiparametric optimization of the relaxin B-chain led to the identification of single chain lipidated peptide agonists of RXFP1 like SA10SC-RLX with subcutaneous bioavailability and extended half-life. SA10SC-RLX has sub nanomolar activity on cells expressing human RXFP1 and molecular modeling associated with the study of different RXFP1 mutants was used to decipher the mechanism of SA10SC-RLX interaction with RXFP1. Telemetry was performed in rat where SA10SC-RLX was able to engage RXFP1 after subcutaneous administration without tachyphylaxis after repeated dosing. Renal blood flow was then used as a translational model to evaluate RXFP1 activation. SA10SC-RLX increased renal blood flow and decreased renal vascular resistance in rats as reported for relaxin in humans. In conclusion, SA10SC-RLX mimics relaxin activity in in vitro and in vivo models of acute RXFP1 engagement. SA10SC-RLX represents a new class of long-lasting RXFP1 agonist, suitable for once daily subcutaneous administration in patients and potentially paving the way to new treatments for chronic fibrotic and cardiovascular diseases.
Exposing intracellular molecular changes during the differentiation of human-induced pluripotent stem cells into erythropoietin-producing cells using Raman spectroscopy and imaging
Authors: Ishigaki Mika , Hitomi Hirofumi , Ozaki Yukihiro , Nishiyama Akira 
Published: November 2022

AbstractThe objective of this study was to explore intracellular molecular changes during the differentiation of human-induced pluripotent stem cells (iPSCs) into erythropoietin (EPO)-producing cells using Raman spectroscopy and imaging. Raman imaging data of fixed cells at four stages of cell differentiation were analyzed by a partial least squares (PLS) regression model, and the variations in the intracellular molecular compositions with cell differentiation were investigated. As a result, three biomarkers characterizing the cell phases were identified: dimethyl sulfoxide (DMSO), fatty acids with a low grade of unsaturation, and glycoproteins. The uptake of DMSO by EPO-producing cells, which was added into a culture medium as an inducer for cell differentiation, was detected, and the increase in unsaturated fatty acid concentrations was revealed that lipid metabolism changed over the course of cell differentiation. The decrease in the glycoprotein concentration after the cell phase during which iPSCs differentiated into EPO-producing cells was also made clear. Raman imaging successfully visualized chemical images of these three biomarkers in two dimensions, where the biomarker concentrations independently varied during cell differentiation. These results demonstrated the application potential of the proposed method to regenerative medicine for monitoring cell differentiation and discriminating cell maturation in situ at the molecular level.
Double-layer polarization-independent achromatic metasurface array for optical fiber bundle coupling in microendoscope
Authors: Sun Yan , Wang Chang , Zheng Shuhang , Tao Xiao , Liu Xinyu , Li Yong , Wu Fei , Zheng Zhenrong 
Published: November 2022

AbstractOptical fiber bundle-based microendoscope, which is significant in clinical diagnosis and industrial detection, calls for miniaturization of the probe and high-resolution observation. Here, we propose a double-layer metasurface array borrowing the structures of insect compound eyes to meet both requirements instead of traditional optical components. Each unit in the array aims for an incident field of view, focusing light at the center of the fiber end face with no chromatic aberration at the wavelengths of 470 nm, 530 nm and 630 nm. The metasurface array is composed of a series of isotropic TiO2 nanopillars which are special selected after considering resonance mode and angular dispersion characteristics, etched on both sides of a silica substrate, with the individual functions of deflecting and focusing. In image space, numerical aperture (NA) is 0.287 and the particular layout of two layers achieve zero telecentricity theoretically, which meet the requirements of optical fiber bundle coupling. A unit for incident angle of 20° is shown to validate our design approach numerically, which obtains a focused spot close to the diffraction limit. The compact and ultrathin metasurface could greatly reduce the size of the probe in optical fiber bundle based microendoscope while ensuring the imaging quality.
A draft chromosome-scale genome assembly of a commercial sugarcane
Authors: Shearman Jeremy R. , Pootakham Wirulda , Sonthirod Chutima , Naktang Chaiwat , Yoocha Thippawan , Sangsrakru Duangjai , Jomchai Nukoon , Tongsima Sissades , Piriyapongsa Jittima , Ngamphiw Chumpol , Wanasen Nanchaya , Ukoskit Kittipat , Punpee Prapat , Klomsa-ard Peeraya , Sriroth Klanarong , Zhang Jisen , Zhang Xingtan , Ming Ray , Tragoonrung Somvong , Tangphatsornruang Sithichoke 
Published: November 2022

AbstractSugarcane accounts for a large portion of the worlds sugar production. Modern commercial cultivars are complex hybrids ofS. officinarum,S. spontaneum, and several otherSaccharumspecies, resulting in an auto-allopolyploid with 8–12 copies of each chromosome. The current genome assembly gold standard is to generate a long read assembly followed by chromatin conformation capture sequencing to scaffold. We used the PacBio RSII and chromatin conformation capture sequencing to sequence and assemble the genome of a South East Asian commercial sugarcane cultivar, known as Khon Kaen 3. The Khon Kaen 3 genome assembled into 104,477 contigs totalling 7 Gb, which scaffolded into 56 pseudochromosomes containing 5.2 Gb of sequence. Genome annotation produced 242,406 genes from 30,927 orthogroups. Aligning the Khon Kaen 3 genome sequence toS. officinarumandS. spontaneumrevealed a high level of apparent recombination, indicating a chimeric assembly. This assembly error is explained by high nucleotide identity betweenS. officinarumandS. spontaneum, where 91.8% ofS. spontaneumaligns toS. officinarumat 94% identity. Thus, the subgenomes of commercial sugarcane are so similar that using short reads to correct long PacBio reads produced chimeric long reads. Future attempts to sequence sugarcane must take this information into account.
Phylogeny and biogeography of Indochinese freshwater mussels in the genus Pilsbryoconcha Simpson, 1900 (Bivalvia: Unionidae) with descriptions of four new species
Authors: Jeratthitikul Ekgachai , Paphatmethin Siwanon , Sutcharit Chirasak , Ngor Peng Bun , Inkhavilay Khamla , Prasankok Pongpun 
Published: November 2022

AbstractThe body of knowledge regarding the classification and evolution of freshwater mussels in the family Unionidae (Bivalvia) in Indochina has recently increased. However, the taxonomic revision of all extant taxa in the region is still ongoing. In this study, the genus Pilsbryoconcha was revised based on an integrative analysis of shell morphology, biogeography, and molecular data. Multi-locus phylogeny indicated the availability of eight species within the genus. Four previously recognized species are P. exilis (Lea, 1838), P. schomburgki (Martens, 1860) stat. rev., P. linguaeformis (Morelet, 1875), and P. carinifera (Conrad, 1837), while four other species are described herein as P. acuta sp. nov., P. mekongiana sp. nov., P. kittitati sp. nov., and P. hoikaab sp. nov. In addition, the neotype of P. carinifera is also designated to clarify its long taxonomic ambiguity. Divergent time estimation and historical biogeography analysis revealed that Pilsbryoconcha originated in the area now called the Khorat Plateau around the middle of the Eocene (mean age = 43.12 Mya), before its range was expanded across Indochina through a series of complex geomorphological changes of river systems, which also led to diversification of the genus.