bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stereotactic body radiation therapy as an effective treatment for pancreatic cancer patients with local advanced stage
Authors: Jia Jun , Zhang Dan , Li Dong , Sun Jing , Li Wen-gang , Duan Xue-zhang 
Published: December 2022

Abstract
Background
To analyze complication and prognosis in pancreatic cancer patients with local advanced stage treated with CyberKnife stereotactic body radiotherapy (CK-SBRT).
Methods
Between November 2018 and November 2021, 31 pancreatic cancer patients with local advanced stage up to our standards were treated by CK-SBRT. The total prescribed doses of 39-66Gy were divided into 6–10 fractions.
Results
By December 2021, among all enrolled patients, 28 patients died, in which 5 patients died of infection of biliary tract (17.86%), 12 of cachexia (31.71%), 2 of liver failure (6.46%), 1 of heart disease (3.23%), 1 of cerebral hemorrhage (3.23%), 7 of other unknown causes. Among the 5 patients died of infection of biliary tract, 4 patients received endoscopic retrograde biliary drainage (ERBD). The overall survival rates of 1-year, 2-year were 32% and 13%, respectively. The progression-free rates of 1-year, 2-year were 85% and 72%, respectively.
Conclusion
CK-SBRT was proved to be an effective option to local advanced stage pancreatic cancer patients who refused resection, chemotherapy or any other therapies, achieving ideal effects. Meanwhile, we conjecture the patients with obstructive jaundice choose percutaneous transhepatic cholangial drainage (PTCD) over endoscopic retrograde biliary drainage (ERBD) to reduce the risk of infection.
DHX9 resolves G-quadruplex condensation to prevent DNA double-strand breaks
Authors: Xue Yuanchao , Chen Juan , Ling Xiaobin , Zhao Youshan , Li Sheng , Li Manman , Zhao Hailian , Yang Xianguang , Tsui Waitang , cao changchang , Wei Lu , Chang Chun-Kang , Zhu Ping , Ma Jinbiao 
Published: December 2022

Abstract
DNA G-quadruplexes (G4s) structures are abundantly present in mammalian genomes and correlated with genome instability. However, the mechanism by which G4s are timely resolved remains unknown. Here, we report that DHX9 functions as a resolvase to unwind G4s globally in activated B cells. DHX9-deficient B cells show gross DNA double-strand breaks at the accumulated G4 sites, which are clustered together and form liquid condensates. We demonstrate that DHX9 also undergoes phase separation and fuses with G4 condensates for the productive unwinding of G4s in an ATP-dependent manner. Physiologically, G4-accumulation-induced DNA breaks can promote immunoglobulin class-switch recombination for producing high-affinity antibodies. Surprisingly, the DHX9Y1189C mutant identified in Hashimoto’s thyroiditis patients shows compromised phase separation and G4 unwinding abilities, causing elevated DNA damage and abnormal antibody production. Our findings suggest a DHX9-dependent G4 condensation-resolving mechanism to prevent DNA damage in mammalian cells. Disrupting this homeostasis may induce autoimmune disorders and lymphoid malignancies.
MiRNA-205-5p Promotes Development of Hepatocellular Carcinoma Via Targeting DLC1
Authors: xu yan , Tang weiming , Lu guang , Ji yin , Huang siyi , Peng ying , Kong xiaoming , Huang Ting 
Published: December 2022

Abstract
Purpose: Researching biomarkers is of great significance for immunotherapy of hepatocellular carcinoma (HCC). The purpose is to research the level and specific function of miR-205-5p in HCC and analyzed related genes, hoping to lay a theoretical basis for HCC treatment.
Methods: The HCC tissues and controls were obtained from hepatocellular carcinoma patients. Human liver immortalized cells (THLE2) and four HCC lines (huh-7, HepG2, HCCLM3, SK-Hep-1) were used in vitro. MiR-205-5p inhibitor, mimic, sh-CD302 Molecule (sh-DCL1) and their corresponding controls were transfected. Starbase and dual-luciferase reporter assay were undertaken for predicting and verifying the binding of miR-205-5p and DLC1. CCK8, EdU, flow cytometry assay was processed to detect cell viability, proliferation and apoptosis. Wound healing and transwell experiment were undertaken to monitor metastasis capability.
Results: There was higher level of miR-205-5p in hepatocellular carcinoma samples. Knockdown of miR-205-5p inhibited the proliferation and improved apoptosis of huh-7 and HepG2 cells. Moreover, miR-205-5p-inhibitor decreased the migration and invasion of HCC cells. MiR-205-5p targeted and negatively adjusted DLC1. Interestingly, sh-DLC1 rescued the influence of miR-205-5p-inhibitor.
Conclusion: MiRNA-205-5p promoted the occurrence and development of HCC via targeting DLC1, which was researched as a potential target for HCC therapy.
Evaluation of Surface Water Quality through CCME-WQI and Multivariate Statistical Techniques
Authors: Akiner Muhammed Ernur , Chauhan Pankaj , Singh Sudhir Kumar 
Published: December 2022

Abstract
The Betwa River Basin, a subbasin of the Yamuna, is the oldest flowing water system in Central India. Factor analysis and principal component analysis (FA/PCA) were multivariate statistical techniques used to extract three and four varimax factors that explained 96.408 and 100.000 percent of the total variance in water quality, respectively. Cluster analysis (CA) categorizes observed items into distinct quality categories based on correlations between stations and years. Point industrial/sewage effluents, non-point runoff from arable land and erosion, and natural source pollution are all factors that contribute to the pollution of the Betwa River, a mineral component of the water. As a result, water quality is threatened or impaired, and conditions often depart from natural or desirable levels at Rajghat, Garrauli, Mohana, and Shahijina stations. According to the Canadian Council of Ministers of the Environment Water Quality Index (CCME-WQI), the water quality ranking at the Rajghat and Mohana stations corresponds to fair ecological status. However, the Garrauli and Shahijina stations' surface water has marginal water quality status. From 1985 to 2018, the Shahijina had the most considerable load of nutrients and organic matter, as established by the CCME-WQI and by comparing the water quality data. A thorough examination revealed a fluctuating trend in the Betwa River pollution, particularly at all stations. Results indicate that between 1985 and 2018, the only defense mechanism of the river was the auto purification mechanism, which is strongly influenced by the drought, point resource of pollution, and extreme meteorological events that probably cause these fluctuations.
Early Childhood Mortality and Associated Factors in Ethiopia: Mini Demographic and Health Survey 2019
Authors: Damete Dereje Derbew , Lucha Tadele Abate 
Published: December 2022

Abstract
Background: Early childhood mortality is a major problem in terms of demographics, health, and development and factors that determine the size and growth rate of a population, as well as its ages and genders, along with its spatial distribution. The aim of this study was to determine factors associated with Early childhood mortality among children aged 0–59 months.
Methods: The data for this study was extracted from Ethiopia mini demographic and health survey 2019. The study involved 5414 women with children aged 0–59 months in a weighted sample. The analysis was performed using STATA version 16 software. Using sampling weights for descriptive statistics and complex sample designs for inferential statistics, backward stepwise elimination was manually applied. Finally, statistical significance declared at the level of p-value < 0.05.
Result: The prevalence of early childhood mortality in Ethiopia was observed to be 5.8%. In multivariate analysis the household wealth index, children from the poorest-wealth index (AOR = 1.211, 95% CI: 0.716-2.047) had higher odds of early childhood mortality in comparison to children from the richer wealth index. The odds of early childhood mortality were thirteen times higher among children born with twins’ birth as compared to single birth, (AOR = 13.615, 95% CI: 6.399-28.965). The odds of early childhood mortality were lower among children delivered in a health facility as compared to home delivery (AOR = 0.936, 95% CI: 0.893-0.982).
The odds of early childhood mortality were lower among tube well sources of drinking water as compared to piped sources of drinking water (AOR = 0.7128, 95% CI: 0.322-1.599). The odds of early childhood mortality were higher among pit latrine-type toilet facilities as compared to flush-type toilet facilities (AOR = 2.056, 95% CI: 1.319-3.207).
Conclusion: The prevalence of early childhood mortality in Ethiopia shows high risk and it is significantly associated with household wealth index, type of birth, place of delivery, sources of drinking water, and types of toilet facilities. Increasing the coverage and access to early childhood education, governmental and non-governmental organizations and all concerned stakeholders should be prioritized as the major determinants of early childhood mortality.
Ripple Effect noted in 15Mon Star using amateur astrophotography equipment.
Authors: Askari Hasan 
Published: December 2022

Abstract
Data collected by Gaia spacecraft has shown the presence of ripple effect in the stars, noted at the outer edge of the Milky-Way. The ripple effect has never been reported to be visible using any ground-based amateur telescope. In this article, I present astronomical images showing ripple effects in Star 15Mon at NGC 2264.
Biomarkers of atopic dermatitis: a meta-analysis over the last decade
Authors: Lyu An-Qi , Lyu Hao-Lin , Wu Chen-Xi , Shan Shi-Jun 
Published: December 2022

Abstract
Objective: A large number of studies had been published over the past decade on the association between the severity of atopic dermatitis and biomarkers. Our purpose is to screen relevant studies and comprehensively evaluate correlations between biomarkers and disease severity, which is an update for the researches in past decade.
Methods: Three databases (PubMed, EMBASE and Cochrane) were searched systematically, and relevant studies were selected according to the inclusion and exclusion criteria.
Results: A total of 49 articles reporting 98 different biomarkers in 4148 patients were rigorously evaluated. Ten markers (serum levels of CCL17, IgE, CCL27, CCL22, IL-13, IL-18, lactate dehydrogenase (LDH), IL-17A, vitamin D, and eosinophils) were used in at least four studies and meta-analyzed.
Conclusions: CCL27, IL-18 and IL-17A were found to be the most reliable biomarkers in the study, with correlation coefficients of 0.62, 0.62 and 0.61, respectively. Other biomarkers that may be useful but require more research include CCL17, IL-13, IgE, LDH, Eosinophil and Vitamin D.
The effects of apelin on IGF1/FSH-induced steroidogenesis, proliferation, Bax expression, and total antioxidant capacity in granulosa cells of buffalo ovarian follicles
Authors: Shokrollahi Borhan , Zheng Hai-Ying , Li Ling-Yu , Tang Li-Ping , Ma Xiao-Ya , Lu Xing-Rong , Duan An-Qin , Huang Chen-Xi , Xu Yuan-Yuan , Shang Jiang-Hua 
Published: December 2022

Abstract
Apelin (APLN) was believed to be an adipokine secreted from adipose tissue. However, studies demonstrate that it is a pleiotropic peptide and has several effects on the female reproductive system. In this study, the effect of different doses of IGF1 and FSH in the presence of APLN-13 on the production of estradiol and progesterone was evaluated in the follicular granulosa cells of buffalo ovaries, in addition, the effects of different doses of APLN isoforms (APLN-13 and APLN-17) on proliferation, the expression of Bax protein and total antioxidant capacity activity of the same cells were investigated. Granulosa cells of buffalo ovaries were cultured in the presence of different doses of IGF1 and FSH with or without APLN-13 (10− 9M) to evaluate its effect on the secretion of estradiol and progesterone that tested by ELISA assay. WST-1 method was used to survey the effect of APLN on granulosa cell proliferation and cytotoxicity. In addition, the antioxidant capacity of the cells in the presence of APLN was assessed using FRAP method. mRNA and Bax protein levels were measured in granulosa cells treated with APLN using real-time PCR and western blot techniques. APLN-13 stimulated the effect of IGF1 on the production of estradiol and progesterone, and the progesterone production levels were affected by APLN-13 dose-dependently. However, it did not significantly stimulate the effect of FSH on the secretion of estradiol or progesterone. APLN-13 (all doses) and − 17 (10− 8 and 10− 9 M) improved the proliferation of granulosa cells. Moreover, preincubation of the cells for an hour by APLN receptor antagonist (ML221, 10 µM) did not significantly affect the proliferation of cells. Neither APLN-13 nor APLN-17 were not cytotoxic for the cells compared to the control treatment. APLN-13 at the doses of 10− 6 and 10− 8 M substantially up and down-regulated Bax protein expression; however, such effects were not observed when the cells were preincubated with ML221. In addition, APLN-17 did not influence the expression amount of Bax. Furthermore, both APLN-13 and − 17 improved the total antioxidant capacity of the ovarian granulosa cells, but such effects were not seen when the cells were preincubated with ML221. These findings indicate that APLN enhanced the IGF1 induced steroidogenesis and improved the cell proliferation and antioxidant capacity of follicular granulosa cells of buffalo ovaries; however, its effect on Bax expression was divergent.
Pharmacogenomic Study—A Pilot Study of the Effect of Pharmacogenomic Phenotypes on the Adequate Dosing of Verapamil for Migraine Prevention
Authors: CHEN YI-CHIEH , Wang Han , Mandrekar Jayawant , Robertson Carrie , Starling Amaal , Cutrer Fred , Chiang Chia-Chun 
Published: December 2022

Abstract
OBJECTIVE
Using individual pharmacogenomic phenotypes to investigate the factors affecting the efficacy and tolerability of verapamil for migraine prevention.
BACKGROUND
Verapamil has a wide range of dosing in headache disorders without reliable tools to predict the optimal doses for an individual.
METHODS
This is a retrospective chart review examining adults with existing pharmacogenomic reports at Mayo Clinic who had used verapamil for migraine. Effects of six cytochrome P450 phenotypes on the minimum effective and maximum tolerable verapamil doses for migraine prevention were assessed.
RESULTS
The mean minimum effective and maximum tolerable doses for the 33 patients in final analysis were 178.2(20–320) mg and 227.9(20–480) mg. A variety of CYP2C9, CYP2D6, and CYP3A5 phenotypes were found, without significant association with the verapamil doses after adjusting for age, sex, body mass index, and smoking status.
CONCLUSIONS
We demonstrated a wide range of effective and tolerable verapamil doses used for migraine in a cohort with various pharmacogenomic phenotypes.
Biosorption of Pb (II) And Ni (II) By Protonated Industrial Waste Yeast Biomass
Authors: Yeneneh Anteneh Mesfin , Kaba Tassisa , Jafary Tahereh , Balushi Khadija Al , Mahrouqi Faisal Al , Adewole Jimoh , Kiamahalleh Meisam Valizadeh , Firouzie Amin 
Published: December 2022

Abstract
This study investigated the biosorption performance of waste yeast biomass derived from brewery for the removal of lead and nickel. Biosorption using low cost waste biomass is known to be an efficient alternative to the conventional technologies to remove heavy metals and organics from industrial effluents. In this study dried ground and protonated yeast used to investigate the removal of Lead (II) and Nickel (II) under the influence several parameters including pH, contact time, presence of co-ions, metal, and sorbent dose. The equilibrium and kinetics of the sorption process and heavy metal recovery tests were also carried out. Significant proportion of the heavy metal ion was sorbed during 5 to 10 minutes of contacting and equilibrium was reached within 60 minutes where the optimum sorption time was observed to be at 30min. Langmuir and Freundlich adsorption models were used in the equilibrium study to fit the equilibrium data after 24 hours of contacting. The kinetics of the metal uptake process was also investigated for varying metal dose in the range of 10-200mg/l and yeast dose in the range of 0.5–4 g/l. qe shows an increasing trend for increasing metals dose and is inversely related to yeast dose, similarly, k and h also showed increasing trend for increasing metal dose. Furthermore, test for ion interference effect or co-ion tests were conducted where sorption of lead decreased from 576 mg/g to 444.5 mg/g when nickel concentration was increased from 50 mg/l to 700 mg/l. Based on recovery tests, the recoverability of lead (80%) was significantly higher than that of nickel (38%). The study has proven that waste yeast industrial biomass can be used for large scale sorption applications in house or in other heavy metal waste generating industries.
High Prevalence of Anxiety Symptoms in Venezuelan Adults during the Beginning of the Venezuelan Crisis. Data from a National Representative Sample. The EVESCAM Study
Authors: González-Rivas Juan P. , Iglesias-Fortes Rocio , De Oliveira-Gomes Diana , Infante-García Maria M. , Durán Maritza , Ugel Eunice , Marulanda María Inés , Novotny Sebastian , Stokin Gorazd B , Mechanick Jeffrey I. , Nieto-Martìnez Ramfis 
Published: December 2022

Abstract
Venezuelans have been living in a humanitarian crisis since 2014 and the effect of this adverse environment on the mental of the population is unknown. This article aims to determinate the prevalence of anxiety and depressive symptoms of adults and factors related during the beginning of the humanitarian crisis of Venezuela. Anxiety and depressive symptoms were determined using the Hospital Anxiety and Depression Scale (HADS). 3,241 adults were evaluated with mean age of 41.1 ± 15.7 years. Anxiety prevalence was 14.7% (women 19.3% and men 9.5%; p < 0.001) and depression prevalence was 3.1% (women 4.0% and men 2.2%; p < 0.001). Independently of age and gender, anxiety was associated with hypertension and tobacco use, and depressive symptoms was associated with smokeless tobacco use, and sedentary lifestyle. Compared to subjects with high/middle socioeconomic status (SES), those with poor and extremely poor SES were more likely to have anxiety, and depression.
Green Synthesis and Catalytic Activity Assessment of Bespoke Nano- Catalyst for Eco-Friendly Green Propellant Systems based on Hydrogen Peroxide
Authors: Elbasuney Sherif , Attwa Mohamed , Deif A. , ElGamal M. , Fayoud Ahmed , Gobara M. 
Published: December 2022

AbstractMuch effort has been devoted to replace pollutant, toxic, cancerogenic hydrazine-based propellants. Hydrogen peroxide could offer promising characteristics as well as high density specific impulse. Effective catalysts with high durability are required to initiate H2O2decompositions, or to inherit hypergolic nature with different fuels. This study reports on the novel green synthesis of silver nanoparticles (18 nm) via hydrogen evolved by water electrolysis. MnO2(20 nm) was developed in a sustainable manner via green hydrothermal processing. High crystalline, mono-dispersed particles were developed. Catalytic activity was assessed via precise measurements of liquid temperature profile (LTP) to reach the boiling point of hydrogen peroxide. Whereas silver demonstrated LTP peak at 20 seconds; MnO2experienced LTP of 40 seconds. Catalyst survivability was recorded via precise measurement of life time mass loss rate upon catalyst addition to hydrogen peroxide. While silver nanocatalyst demonstrated high performance at the reaction start; silver was found to be poisoned with crystalline phase change to silver oxide within 20 seconds. On the other hand, manganese oxide experienced high durable catalytic action. Consequently, MnO2could be candidate for H2O2monopropellant thrusters as catalyst bed. On the other hand, silver nanoparticles could be candidate for a single use in bipropellant to inherit hypergolicity.
Investment-grade corporate bond as the top-performing asset class and its safe-haven role during the COVID-19 pandemic
Authors: Wang Qiyu 
Published: December 2022

Abstract
In this paper, we compare asset classes performance quantitatively, including worldwide stock indices, bonds of different grades, gold, energy and foreign exchange. Bonds, especially investment-grade corporate bonds (Moody’s DBAA), performed the best among all the asset classes. Prices plunged significantly in Phase I, from COVID-19 start to March 2020and re-bounced after CARES (Coronavirus Aid, Relief, and Economic Security). We thus analyze the quantitative results through subperiods including pre-COVID-19 subperiod, Phase I, Phase II and time after lockdown and also compare to situations in the subprime crisis. The COVID-19 period asset correlations among asset classes increased than the usual. Since the means and variances of asset returns are not stationary, dynamic conditional correlation (DCC) model computes correlations between DBAA and other assets. Test results of the Phase I, Phase II, or extreme quantiles dummy variables’ impacts on the DCC time series confirm DBAAs safe-haven role.
Association of the Renin-Angiotensin System Components in Human Follicular Fluid with Age, Ovarian Function and IVF Outcome: A Cross-Sectional Study
Authors: Wei Lun , Bo Le , Zhou Anwen , Luo Chao , Wu Zhinan , Lu Xuanping , Zhan Lei , Gao Shasha , Qian Fei , Mao Caiping 
Published: December 2022

Abstract
Background
Previous studies have found that there is the local renin-angiotensin system (RAS) in human ovary, and it independently plays a regulatory role in ovarian function. Age-related reproductive aging is an inevitable process. To explore the relationship between RAS components in human follicular fluid (hFF) and age, ovarian function, so as to help clinical evaluation of oocyte quality and prediction of in vitro fertilization (IVF) outcome.
Methods
hFF of 139 women who received IVF simply because of their husband's factors from January 2021 to February 2022. The levels of RAS components in hFF were measured, including Renin, ACE, ACE2, AngⅡ and Ang(1–7). The correlation between age and RAS in hFF was analyzed by simple linear regression, and multivariate linear regression was used to further analyze the correlation between the RAS and IVF outcome.
Results
A total of 139 samples of analysable hFF were obtained in this study. It was found that there was a linear negative correlation between age and renin, ACE, AngⅡ, ACE/ACE2, AngⅡ/Ang(1–7) (Pearson's r < 0, P < 0.05), while the linear relationship with ACE2 and Ang (1–7) was not significant (P > 0.05). It was found that bFSH and bLH were correlated with age, ACE-AngⅡ-AT1/2R axis, ACE2-Ang(1–7)-MAS axis (P < 0.05), AFC was correlated with age, ACE2-Ang(1–7)-MAS axis (P < 0.05), and bAMH was only correlated with age (P < 0.01). There was no correlation between RAS and bE2, bP, bPRL, bT (P > 0.05). It was found that the number of oocyte retrieval and MⅡ maturation rate were correlated with age, renin, ACE-AngⅡ-AT1/2R axis and ACE2-Ang(1–7)-MAS axis (P < 0.05), and the rate of 2PN embryos, transplantable embryos, high-quality embryos were only correlated with age (P < 0.01).
Conclusions
①The influence of age on ACE-AngⅡ-AT1/2R axis was more significant than that on ACE2-Ang(1–7)-MAS axis in hFF; ②Provides evidence that gonadotropins mediate RAS activation of local ovarian follicles; ③It is still impossible to prove the effect of RAS on steroid hormone production, at least in ovarian follicles RAS cannot affect steroid hormones in the systemic circulation; ④Age and the level of RAS components in hFF have certain clinical value in predicting the number of oocyte retrieval and the rate of MⅡ maturation.