bluuebunny/crossref_metadata_2025_split huggingface dataset:
An integrated analysis of C5AR2 related to malignant properties and immune infiltration of gliomas
Authors: Huang Chengying , Qiu Ouwen , Mao Chaofu , Hu Zhicheng , Qu Shanqiang 
Published: October 2022

AbstractBackgroundC5AR2 is recognized as a proinflammatory molecule and activates the inflammatory response in multiple disorders. However, little has been reported on C5AR2 in glioma. This study sought to explore its expression, biological function, and association with clinical pathological indicators, prognosis, and immune infiltration levels in glioma through glioma cohorts.MethodsA cohort of 657 patients was screened from the Chinese Glioma Genome Atlas (CGGA). χ2 test was performed to calculate the difference of classified variables. Cox proportional hazard regression modeling was used to identify independent prognostic indicators of glioma patients. A survival plot was generated by the Kaplan–Meier method. The immune cell infiltration score of glioma patients was calculated by TIMER algorithm.ResultsWe observed that high expression of C5AR2 was strongly associated with malignant clinical indicators in 657 patients with glioma, and patients with high C5AR2 expression had worse prognoses. Multivariate Cox analysis showed that C5AR2 could be a new independent prognostic indicator for glioma patients. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that C5AR2 overexpression correlated with multiple inflammatory and immune biological processes. Additionally, high C5AR2 expression was strongly associated with higher abundance and marker gene expression of multiple tumor immune cells in low‐grade glioma. Finally, a model was constructed to improve the prognostic evaluation of glioma patients.ConclusionsThe C5AR2 gene is highly expressed in gliomas and is significantly associated with clinical indicators of malignant progression in glioma patients. In glioma, patients with high C5AR2 expression displayed a worse outcome. In glioma tissues, the expression level of C5AR2 highly correlated with the abundance of tumor immune cell infiltration. Additionally, GO and KEGG enrichment analysis revealed that C5AR2 expression may be involved in a variety of immune and inflammatory biological processes.
Prognostic awareness and its association with health outcomes in the last year of life
Authors: Ozdemir Semra , Chaudhry Isha , Ng Sean , Teo Irene , Malhotra Chetna , Finkelstein Eric Andrew ,   COMPASS Study Group
Published: October 2022

AbstractBackgroundLittle research has examined changes in prognostic awareness (PA) in the last year of life and the extent PA change was associated with anxiety, depression, and spiritual well‐being among metastatic cancer patients.MethodsTwo surveys were administered in the last year of life to 176 conveniently sampled Singaporean patients with stage 4 solid cancers. PA was assessed by asking patients whether they were aware that their treatments were unlikely to cure their cancer. Multivariable linear regression models were used to investigate the association of PA change with anxiety, depression, and spiritual well‐being.ResultsThe proportion of patients with accurate PA increased (39.2%–45.5%; p < 0.05) from the second‐last assessment to the last assessment before death. Those with inaccurate PA decreased (26.1%–20.4%; p < 0.05) while a third of patients remained uncertain at both assessments (34.7% and 34.1%). Compared to patients with inaccurate PA at both assessments, patients who reported accurate PA at both assessments reported worsened anxiety (β = 2.08), depression (β = 3.87), and spiritual well‐being (β = −4.45) while patients who reported being uncertain about their prognosis at both assessments reported worsened spiritual well‐being (β = − 6.30) at the last assessment before death (p < 0.05 for all).ConclusionsInterventions should dually focus on decreasing prognostic uncertainty at the end‐of‐life while minimising the psychological and spiritual sequelae associated with being prognostically aware. More research is needed to clarify the causes of prognostic uncertainty.
Cisplatin‐induced HSF1‐HSP90 axis enhances the expression of functional PD‐L1 in oral squamous cell carcinoma
Authors: Sasaya Takashi , Kubo Terufumi , Murata Kenji , Mizue Yuka , Sasaki Kenta , Yanagawa Junko , Imagawa Makoto , Kato Hirotaka , Tsukahara Tomohide , Kanaseki Takayuki , Tamura Yasuaki , Miyazaki Akihiro , Hirohashi Yoshihiko , Torigoe Toshihiko 
Published: October 2022

AbstractImmune checkpoint inhibitor‐based cancer immunotherapy has provided an additional therapeutic option for oral squamous cell carcinoma (OSCC) with recurrence or distant metastases. However, further improvement of OSCC treatment is required to develop the optimal combination or order for chemoradiotherapy and immunotherapy. Along with the accumulation of clinical knowledge and evidence, it is also essential to clarify the biological impact of chemo‐radiotherapeutic agents on the cancer immune microenvironment. In this study, we investigated the effects of cisplatin (CDDP), a key therapeutic agent for OSCC, on programmed death‐ligand 1 (PD‐L1) expression in OSCC lines. Although CDDP treatment increased the surface levels of PD‐L1 on OSCC cell lines, the gene and total protein expression levels of PD‐L1 were not altered. We also demonstrated that the phosphorylation of heat shock factor 1 and heat shock protein 90 was involved in this process. In addition, CDDP‐induced PD‐L1 attenuated the target‐specific cytotoxic T lymphocyte reaction to OSCC. These results provide an immunobiological basis for the response of OSCC to CDDP and will contribute to our biological understanding of the action of novel combination therapy including immunotherapy together with platinum‐based chemotherapy for OSCC.
Inhibition of XPO1 impairs cholangiocarcinoma cell proliferation by triggering p53 intranuclear accumulation
Authors: Zhao Cheng , Ma Ben , Yang Zi‐yi , Li Ou , Liu Shi‐lei , Pan Li‐jia , Gong Wei , Dong Ping , Shu Yi‐jun 
Published: October 2022

AbstractBackgroundXPO1 mediates the nuclear export of several proteins, mainly tumor suppressors. KPT‐330 (Selinexor) is a selective inhibitor of XPO1 that has demonstrated good therapeutic effects in hematologic cancers.MethodsWe used TCGA and GTEx pan‐cancer database to evaluate XPO1 mRNA expression in various tumors. Cell proliferation assay and colony formation assay were used to analyze the in vitro antitumor effects of XPO1 inhibitor KPT‐330. Western blot was performed to explore the specific mechanisms.ResultsWe found that XPO1 was highly expressed across a range of cancers and associated with poor prognosis in hepatobiliary and pancreatic tumors. We revealed that the XPO1 inhibitor KPT‐330 triggered the nuclear accumulation of the p53 protein and significantly disrupted the proliferation of cholangiocarcinoma cells. Mechanistically, the XPO1 inhibitor, KPT‐330, reduced BIRC6 expression by inhibiting the PI3K/AKT pathway to decrease p53 degradation and improve its stability.ConclusionTherefore, XPO1 may be a potential therapeutic target in cholangiocarcinoma, mediated by its effects on KPT‐330.
RUNX2 interacts with SCD1 and activates Wnt/β‐catenin signaling pathway to promote the progression of clear cell renal cell carcinoma
Authors: Song Xiandong , Liu Junlong , Liu Bitian , Piao Chiyuan , Kong Chuize , Li Zhenhua 
Published: October 2022

AbstractBackgroundPrevious studies have demonstrated that Runt‐associated transcription factor 2 (RUNX2) serves as the main transcription factor for osteoblast differentiation and chondrocyte maturation. RUNX2 is related to a variety of tumors, particularly tumor invasion and metastasis, while the expression and molecular mechanisms of RUNX2 in clear cell renal cell carcinoma (ccRCC) keep to be determined. Stearyl CoA desaturase 1 (SCD1), an endoplasmic reticulum fatty acid desaturase, transfers saturated fatty acids to monounsaturated fatty acids, is expressed highly in numerous malignancies.MethodsThe Cancer Genome Atlas (TCGA) datebase and Western blot was used to analyzed the mRNA and protein levels of the target gene in ccRCC tissues and adjacent tissues. The proliferation ability of ccRCC cells was tested by colony forming and EdU assay. The migration ability of cells was detected by transwell assay. Immunoprecipitation was utilized to detect protein–protein interaction. Cycloheximide chase assay was used to measure the half‐life of SCD1 protein.ResultsIn this study, the expressions of RUNX2 and SCD1 are increased in ccRCC tissues as well as ccRCC cell lines. Both RUNX2 and SCD1 could promote proliferation and migration in ccRCC cells. Furthermore, RUNX2 could physically interact with SCD1. In addition, the functional degradation and the inactivation of Wnt/β‐catenin signaling pathway triggered by the downregulation of RUNX2 could be partly offset by the overexpression of SCD1.ConclusionThe findings indicate that the RUNX2/SCD1 axis may act as a potential therapeutic target via the Wnt/β‐catenin signaling pathway of ccRCC.
Lignans and Flavonoids from Cajanus cajan (L.) Millsp. and Their α‐Glucosidase Inhibitory Activities
Authors: Lei Xin‐Yu , Liu Shao‐Huan , Zhao Ya‐Xian , Guo Meng‐Jia , Tao Ling , Shen Xiang‐Chun , Zhang Nen‐Ling 
Published: October 2022

AbstractA pair of new lignan conformers (1–2), one new flavonoid glycoside (3), as well as nineteen known compounds were purified from the twigs and leaves of Cajanus cajan (L.) Millsp.. The planar structures of the unknown compounds were determined via NMR and high‐resolution mass spectrometry, while their absolute configurations were elucidated via comparison between their experimental and calculated electronic circular dichroism (ECD) values. All the isolated compounds were assayed for their α‐glucosidase inhibitory activities. The results demonstrated that compounds 8–12, 15–16, 18–19, 21–22 had strong inhibition activities, with compound 10 (IC50=0.4±0.21 μM) most active. The structure‐activity relationships were preliminarily summarized. Enzyme kinetics showed that compounds 8, 9, 15–16, 18–19, 21–22 were non‐competitive inhibitors and compounds 10–12 were anti‐competitive ones.
Anticholinesterase Compounds from Endemic Prangos uechtritzii
Authors: Albayrak Gokay , Demir Serdar , Koyu Halil , Baykan Sura 
Published: October 2022

AbstractIn this study, the anticholinesterase effects of the extracts and isolated compounds from the roots of endemic Prangos uechtritzii Boiss & Hausskn (Apiaceae) are reported. A novel polyacetylenic compound; (+)‐8‐O‐methyloplopantriol A along with two known polyacetylenes; (−)‐panaxynol, (+)‐falcarindiol and fifteen known coumarin derivatives; umbelliferone, 6‐formylumbelliferone, suberosin, 7‐demethylsuberosin, (+)‐ulopterol, tamarin, psoralen, imperatorin, (+)‐oxypeucedanin, (+)‐oxypeucedanin hydrate, (+)‐oxypeucedanin methanolate, (+)‐marmesin, (−)‐prantschimgin, (+)‐decursinol, and (−)‐adicardin were isolated from the hexane (Pu‐HE), chloroform (Pu‐CE), and methanol (Pu‐ME) extracts of P. uechtritzii roots. (−)‐Panaxynol, (+)‐falcarindiol, 6‐formylumbelliferone, (+)‐decursinol, and (−)‐adicardin were obtained from the genus Prangos for the first time. (+)‐8‐O‐Methyloplopantriol A inhibited both AChE (IC50=194.5±5.8 μM) and BChE (IC50=51.9±2.96 μM) enzymes. (+)‐Falcarindiol, 6‐formylumbelliferone, 7‐demethylsuberosin, tamarin, and imperatorin also exhibited BChE‐specific inhibitory activities (IC50=27.88‐93.86 μM). (+)‐Falcarindiol (IC50=27.88±0.91 μM) and imperatorin (IC50=30.89±1.40 μM) as the most active components could be led compounds to develop new BChE inhibitors with further research against Alzheimer's disease.
Recent Advances towards the Reversible Chemical Modification of Proteins
Authors: Wu Chuan‐Shuo , Cheng Liang 
Published: October 2022

AbstractProteins are intriguing biomacromolecules for all living systems, not only as essential building blocks of organisms, but also as participants in almost every aspect of cellular activity such as metabolism and gene transcription/expression. Developing chemical biology tools that are capable of labeling/modifying proteins is a powerful method for decoding their detailed structures and functions. However, most current approaches heavily rely on the installation of permanent tags or genetic engineering of unnatural amino acids. There has been slow development in reversible chemical labeling using small organic probes and bioorthogonal transformations to construct site‐selectively modified proteins and conditionally restore their activities or structures. This review summarizes recent advances in the field of chemical regulation of proteins with reversible transformations towards distinct motifs, including amino acid residues, amide backbones and native post‐translational lysine. Finally, current challenges and future perspectives are discussed.
SIRT1 enhances oxaliplatin resistance in colorectal cancer through microRNA‐20b‐3p/DEPDC1 axis
Authors: Zhao Bin , Wang Yuncui , Zhao Xingwang , Ni Jian , Zhu Xiaowen , Fu Yan , Yang Fan 
Published: October 2022

AbstractOxaliplatin (L‐OHP) is a standard treatment drug for colorectal cancer (CRC), but acquired drug resistance limits the outcome of patients. We investigated the involvement of sirtuin 1 (SIRT1) in L‐OHP resistance in the setting of CRC via microRNA‐20b‐3p/DEP domain containing 1 (miR‐20b‐3p/DEPDC1) axis. CRC tissues that were resistant or sensitive to L‐OHP were harvested, in which SIRT1, miR‐20b‐3p, and DEPDC1 levels were tested. L‐OHP‐resistant‐resistant CRC cells were transfected, subsequently, cellular proliferation, invasion, migration, and apoptosis were tested, and tumor resistance to L‐OHP was observed. The binding of SIRT1 to miR‐20b‐3p promoter and the targeting relationship between miR‐20b‐3p and DEPDC1 were verified. An aberrant elevation in SIRT1 expression was seen in L‐OHP‐resistant CRC tissues and cells. Knockdown of SIRT1 sensitized CRC cells and xenografted CRC tumors to L‐OHP. SIRT1 bound with miR‐20b‐3p promoter to regulate DEPDC1. Reducing miR‐20b‐3p or raising DEPDC1 levels weakened the effect of SIRT1 knockdown on L‐OHP‐resistant‐CRC cells. SIRT1 enhances L‐OHP resistance in CRC by mediating miR‐20b‐3p/DEPDC1 axis.
Bioinformatics analysis of markers based on m6A related to prognosis combined with immune invasion of renal clear cell carcinoma
Authors: Liu Lian , Zhuang Meng , Tu Xin‐hua , Li Cheng‐cheng , Liu Hong‐hui , Wang Jing 
Published: October 2022

AbstractThe incidence rate of renal cell carcinoma (RCC) is about 3% of all adult cancers. Of these, the Kidney clear cell renal cell carcinoma (KIRC) is the most common type, accounting for about 70%–75% of RCC. KIRC is difficult to be detected in time clinically. KIRC still has no effective treatment at this stage. We combined high‐throughput bioinformatics analysis to obtained the structural sequence transcriptome data, relevant clinical information, and m6A gene map of KIRC patients from genomics TCGA database. Pearson's correlation analysis was used to explore m6A related gene long noncoding RNAs (lncRNAs), and then univariate Cox regression analysis was performed to screen the prognostic role of KIRC patients. Lasso–Cox regression was performed to establish the lncRNAs risk model associated with m6 A.LINC02154 and AC016773.2, Z98200.2, AL161782.1, EMX2OS, AC021483.2, CD27‐AS1, AC006213.3 were iidentif. Compared with the low‐risk group, the overall survival of patients in the high‐risk group was significantly worse. Analyzing whether there are differences in immune cells between high‐risk and low‐risk subgroups. There were CD4 memory resting, Monocytes, Macrophages M1, Dendritic cells activated, Mast cells resting, which had higher infiltrations in the low‐risk group. We performed Go enrichment analysis, Kyoto Encyclopedia of Genes and Genomes enrichment analysis and gene set enrichment analysis enrichment analysis. Overall, our results suggest that the component of m6A‐related lncRNAs in the prognostic signal may be a key mediator in the immune microenvironment of KIRC, which represents a promising therapeutic effect.
A case of Huntington disease‐like 2 in a patient of African ancestry: the everlasting support of clinical examination in the molecular era
Authors: Ruscitti Federica , Origone Paola , Rosti Giulia , Trevisan Lucia , Marchese Roberta , Brugnolo Andrea , Massa Federico , Castellini Paola , Mandich Paola 
Published: October 2022

AbstractChorea, cognitive decline, and psychiatric symptoms are shared by Huntington’s disease (HD) and similar conditions called HD phenocopies. We describe the first case reported in Italy of Huntington disease‐like 2 (HDL2), clinically and radiologically indistinguishable from HD, showing the importance of considering African ancestry in the diagnostic process.
Phase/Size‐Controlled Synthesis of Nanostructured PbO2Microspheres for Efficient Electrochemical Ozone Generation
Authors: Yu Qiuyang , Hu Xiaohong 
Published: October 2022

AbstractTraditional electrochemically prepared lead dioxide (e‐PbO2) is one of the most used electrode materials for electrochemical ozone production (EOP), because of its high catalytic activity, excellent electrical conductivity, relative stability and low cost. But the electrochemically prepared conditions are complicated and harsh. Bulk PbO2electrodes have the very limited surface area and are inconvenient to process into the fine film electrodes for a PEM electrolyser. In contrast, chemical synthesis is easy to prepare uniform PbO2catalysts with high surface area and catalytic activity. Here, a facile one‐pot approach to prepare a highly active PbO2microsphere catalyst was established and used for the EOP reaction in a PEM ozone generator. Sodium hydroxide could be used to regulate the relative content of α/β allotropic phase and crystallite size of PbO2microspheres. Experiment results revealed that high β phase content and large β‐PbO2crystallite size were beneficial to the high EOP activity. By optimizing the composition and crystal structure, chemically prepared PbO2could have much better EOP performance than e‐PbO2.
Continuous‐Flow Divergent Lithiation of 2,3‐Dihalopyridines: Deprotolithiation versus Halogen Dance
Authors: Brégent Thibaud , Ivanova Maria V. , Poisson Thomas , Jubault Philippe , Legros Julien 
Published: October 2022

AbstractWe describe herein the first halogen dance (HD) in continuous flow on 2‐chloro‐3‐bromopyridine by selectively trapping a (pyridin‐4‐yl)lithium species that is known to undergo the halogen‐dance process. In addition, this lithiated intermediate was trapped at lower temperature before the HD occurs. The HD process was extended to fluoro‐iodopyridines by using various electrophiles to afford 28 examples with yields ranging from 42 to 97 % with very short residence times. Finally, scale up of the reaction was demonstrated, affording a promising space‐time yield (STY) of 4.2 kg.h−1.L−1.
Pentacyclic and Hexacyclic Osmaarynes and Their Derivatives
Authors: Fei Yang Xiao , Zhang Ming‐Xing , Bin Fu De , Wang Yang , Yin Jun , Hua Liu Sheng 
Published: October 2022

AbstractAlthough osmabenzyne, osmanaphthalyne, osmaphenanthryne, and osmaanthracyne have been previously reported, the synthesis of polycyclic osmaarynes is still a challenge. Herein, we report the successful synthesis of the first pentacyclic osmaarynes (pyreno[b]osmabenzynes1 aand2 a) and hexacyclic osmaaryne (peryleno[b]osmabenzyne3 a). Nucleophilic reaction of osmaarynes was used to obtain the corresponding pyreno[b]osmium complexes (1and2) and peryleno[b] osmium complex (3), which exhibited near‐infrared luminescence and aggregation‐induced emission (AIE) properties. Complexes2and3are resistant to photodegradation, and complex2has better photothermal conversion properties than3.
Size Selectivity in Heterolanthanide Molecular Complexes with a Ditopic Ligand
Authors: Bellucci Luca , Fioravanti Lorenzo , Armelao Lidia , Bottaro Gregorio , Marchetti Fabio , Pineider Francesco , Poneti Giordano , Samaritani Simona , Labella Luca 
Published: October 2022

AbstractThe similar reactivity of lanthanides generally leads to statistically populated polynuclear complexes, making the rational design of ordered hetero‐lanthanide compounds extremely challenging. Here we report on the site selectivity in hetero‐lanthanide tetranuclear complexes afforded by the relatively simple ditopic pyterpyNO ligand (4’‐(4‐pyridil)‐2,2’:6’,2”‐terpyridine N‐oxide). The sequential room temperature reaction of RE2(tta)6(pyterpyNO)2 (where RE=Y, (1); Eu, (2), Dy, (3) Htta=2‐thenoyltrifluoroacetone) with La(tta)3dme (dme=dimethoxyethane) yielded Y2La2(tta)12(pyterpyNO)2 (4), Dy2La2(tta)12(pyterpyNO)2 (5) and Eu2La2(tta)12(pyterpyNO)2 (6). Single crystals X‐ray diffraction studies showed that 4, 5 and 6 are isostructural, featuring a tetranuclear structure with two different metal coordination sites with coordination numbers 8 (CN8) and 9 (CN9). The two smaller cations are mainly bridged by the O‐donor atoms of the NO groups of two pyterpyNO ligands (CN8), while the larger lanthanum centres are bound by a terpyridine unit (CN9). Size selectivity has been studied with structural and magnetic studies in the solid state and through 19F NMR and photoluminescence studies in solution, showing a direct dependence on the difference of ionic radii of the ions and yielding a 91 % selectivity for 4. Furthermore, 19F NMR, X‐ray and PL studies pointed out that the nature of the product is independent from the synthetic route for compound Eu2Y2(tta)12(pyterpyNO)2 (7), keeping the ion selectivity also for a self‐assembly reaction. Unexpectedly, these studies have evidenced that selectivity is not exclusively governed by electrostatic interactions related to size dimensions.
The Richardson‐Zaki Equation Reconsidered: Derivation of the Power Law for Aerated Particle Beds
Authors: Hristov Jordan 
Published: October 2022

AbstractAnalytical derivations of the Richardson‐Zaki power law in the case of aeratable fine particle beds where the interparticle forces allow the creation of expanded beds, known also as meta‐states, with fixed structures, but not yet fluidized, have been developed. The analysis is based on the concept that the fluid flow through such beds is governed by the porous media equations such as Darcy's and Forchheimer models, upon the assumption of deformable bed structures (driven by the fluid flow) and fluid velocity independent pressure drop.
A random walk through Canadian contributions on empirical processes and their applications in probability and statistics
Authors: Csörgő Miklós , Dawson Donald A. , Nasri Bouchra R. , Rémillard Bruno N. 
Published: October 2022

AbstractIn this article, we present a review of important results and statistical applications obtained or generalized by Canadian pioneers and their collaborators, for empirical processes of independent and identically distributed observations, pseudo‐observations, and time series. In particular, we consider weak convergence and strong approximations results, as well as tests for model adequacy such as tests of independence, tests of goodness‐of‐fit, tests of change point, and tests of serial dependence for time series. We also consider applications of empirical processes of interacting particle systems for the approximation of measure‐valued processes.