bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary Total Laryngectomy versus Organ Preservation for Locally Advanced T3/T4a Laryngeal Cancer
Authors: Lee Maxwell Y. , Belfiglio Mario , Zeng Johnathan , Fleming Christopher W. , Koyfman Shlomo , Joshi Nikhil P. , Lamarre Eric , Prendes Brandon , Scharpf Joseph , Lorenz Robert R. , Woody Neil M. , Adelstein David J. , Geiger Jessica L. , Chute Deborah J. , Ku Jamie A. 
Published: June 2022

ObjectiveOrgan preservation (OP) treatment for advanced laryngeal cancer has increased compared to primary total laryngectomy. Our study compares oncologic and functional outcomes between these approaches.Study DesignRetrospective cohort study.SettingSingle tertiary care institution.MethodsRetrospective review of patients receiving primary total laryngectomy or OP for laryngeal cancer between 1/1/2000 and 12/31/2018.ResultsA total of 118 patients received primary total laryngectomy and 119 received OP. Overall survival was similar between total laryngectomy and OP. When stratified by T stage, disease‐free survival was worse among T3 patients receiving OP versus total laryngectomy. In T3 patients, 28 OP patients experienced local recurrence (28.9%) compared to 3 total laryngectomy patients (7.1%; p < 0.01). In total, 20 OP patients with local recurrence received salvage surgery. These patients had similar overall survival to patients who underwent initial total laryngectomy (TL). About 14 OP patients with local recurrence did not receive salvage surgery. About 89 (75.4%) TL patients achieved normal diet as compared to 64 (53.8%) OP patients (p < 0.001). In TL patients, 106 (89.8%) received primary or secondary tracheoesophageal‐prosthesis, 82 (77.4%) of whom achieved completely understandable speech.ConclusionsThere was no difference in survival by treatment in T4 patients, possibly because of strict patient selection. However, disease‐free survival was worse in T3 patients receiving OP, likely due to a high local recurrence rate. Approximately 40% of patients with local recurrence were not eligible for salvage laryngectomy. TL patients had comparable swallowing and speech outcomes with OP patients.Level of Evidence3 Laryngoscope, 133:1122–1131, 2023
Extracellular vesicles: Emerging frontiers in wound healing
Authors: Hade Mangesh D. , Suire Caitlin N. , Mossell James , Suo Zucai 
Published: June 2022

AbstractExtracellular vesicles are membranous particles, ranging from 30 nm to 10 µm in diameter, which are released by nearly all cell types to aid in intercellular communication. These complex vesicles carry a multitude of signaling moieties from their cell of origin, such as proteins, lipids, cell surface receptors, enzymes, cytokines, metabolites, and nucleic acids. A growing body of evidence suggests that in addition to delivering cargos into target cells to facilitate intercellular communication, extracellular vesicles may also play roles in such processes as cell differentiation and proliferation, angiogenesis, stress response, and immune signaling. As these vesicles have natural biocompatibility, stability in circulation, low toxicity, and low immunogenicity, and serve as efficient carriers of molecular cargos, these nanoparticles are ideal therapeutic candidates for regenerative medicine. Exploring and identifying the homeostatic functions of extracellular vesicles may facilitate the development of new regenerative therapies. In this review, we summarize the wound healing process, difficulties in stem cell therapies for regenerative medicine, and the applications of mesenchymal stromal cell‐derived extracellular vesicles in improving and accelerating the wound healing process.
Comparison of transfusion reactions in children and adults: A systematic review and meta‐analysis
Authors: Wang Yaru , Sun Wenjie , Wang Xing , Ren Xiaoyan , Gao Ang , Li Meng , Wang Xiaowei 
Published: June 2022

AbstractBackgroundThere are no international standards or normalizations for diagnosing and treating complications from blood transfusions. We comprehensively compared the incidence of adverse blood transfusions in children and adults.MethodsAvailable literature on blood transfusion adverse reactions in children and adults prior to November 27, 2021 was collected from several electronic databases. This meta‐analysis was performed using Revman 5.2 and Stata 15.1.ResultsThe incidence of transfusion reactions is higher in children than in adults. Children transfused with red blood cells and platelets exhibited a higher incidence of transfusion reaction than that of adults. Moreover, the incidence of allergic and febrile non‐hemolytic transfusion reactions was significantly higher in children than in adults. The incidence of some rare transfusion reactions was also significantly higher in children than in adults.ConclusionThe incidence of transfusion reactions in children and adults is varied. Guidelines for children are necessary.
Toward integrated pest management in bivalve aquaculture
Authors: Cahill Patrick L , Davidson Ian C , Atalah Javier A , Cornelisen Chris , Hopkins Grant A 
Published: June 2022

AbstractPests of bivalve aquaculture are a challenging problem that can reduce productivity, profitability and sustainability. A range of pest management approaches have been developed for bivalve aquaculture, but a general absence of guiding frameworks has limited the scale and permanency of implementation. Applying principles of ‘integrated pest management’ (IPM) could change this paradigm to improve economic and environmental outcomes. We reviewed existing research and tools for pest management in bivalve aquaculture, with studies grouped under five pillars of IPM: pest ecology (25 studies), bioeconomic cost–benefits (4 studies), continual monitoring (17 studies), proactive prevention (32 studies) and reactive control (65 studies). This body of knowledge, along with insights from terrestrial agriculture, provide a strong foundation for developing and implementing IPM in bivalve aquaculture. For example, IPM principles have been applied by a regional collective of oyster farmers in the US Pacific Northwest to optimize pesticide application and search for other options to control problematic burrowing shrimps. However, IPM has not yet been broadly applied in aquaculture, and data gaps and barriers to implementation need to be addressed. Priorities include establishing meaningful pest–crop bioeconomic relationships for various bivalve farming systems and improving the efficacy and operational scale of treatment approaches. An IPM framework also could guide potential step‐change improvements through directing selective breeding for resistance to pests, development of bespoke chemical control agents, applying emerging technologies for remote surveillance and farm management, and regional alignment of management interventions. © 2022 Society of Chemical Industry.
A review of leopard seal (Hydrurga leptonyx) births and pups using a standardised age-class classification system
Authors: van der Linde Krista , Visser Ingrid N. , Bout Rick , Krause Douglas J. , Forcada Jaume , Siniff Donald , Stone Sheridan , Fyfe Jim , Fernández-Ferrada Nicolás , Macallan Kathryne , Savenko Oksana , Cooper Tracy E. 
Published: June 2022

AbstractDespite the ecological importance of leopard seals (Hydrurga leptonyx) as apex marine predators, little is known about their reproductive biology. To address this paucity, we reviewed leopard seal birth and pup records and applied a standardised age-class classification system to differentiate between births/newborns (offspring ≤ 14 days old) and pups (> 14 days but <  6 months old). We compiled 19 birth/newborn and 141 pup records and examined their occurrence by month, region, substrate, birth-specific attributes (i.e. birth observations, fresh umbilicus or placental), standard length, weight, presence of mother, presence of lanugo, sex, status (e.g. born alive) and fate. These records indicate that leopard seal births occur between September and December, with peak records from September to November, whilst pup records peaked between August and December. The regions with the most birth/newborn records were the sub-Antarctic Islands (31.6%) and Chile (31.6%), followed by Antarctica (15.8%), New Zealand (15.8%) and the Falkland Islands (5.3%). Pups were recorded predominantly in the sub-Antarctic Islands (54.6%), followed by the Antarctic (42.6%), Chile (2.1%) and Australia (0.7%). Whilst leopard seal birth records were predominantly on ice, they were also found onterra firma. The northernmost published leopard seal birth records occurred in New Zealand whilst the northernmost published leopard seal pup records occurred in Australia. This study contradicts the long-standing hypothesis that leopard seals only give birth on Antarctic pack ice, and instead, here we indicate that 84.2% and 57.4% of collated leopard seal birth and pup records, respectively, occur outside of Antarctica. Our records illustrate the importance of northern regions as part of the leopard seal’s range. We emphasise the need to conduct research focused on the reproductive biology of this keystone species throughout its range and that future management of leopard seal populations should also consider their northern range.
Urine podocyte mRNA loss in preterm infants and related perinatal risk factors
Authors: Gao Qi , Lu Congchao , Tian Xiuying , Zheng Jun , Ding Fangrui 
Published: June 2022

Abstract

Background
Preterm birth has been identified as a risk factor for development of long-term chronic kidney disease. Podocyte loss has been reported to contribute to this process in preterm animal models. However, details about podocyte loss in preterm infants and related perinatal risk factors have not been well clarified.

Methods
Forty full-term infants and 106 preterm infants were enrolled. Urine samples were collected from full-term infants within 4–7 days of birth and preterm infants at 37–40 weeks of corrected age. Levels of urine podocin mRNA, urine protein (UP), and urine microalbumin (UMA) were measured, and the relationship between these markers was evaluated. Clinical information in these infants was collected, and potential correlates that may lead to increased podocyte loss during the perinatal period were identified using linear regression analysis.

Results
Urine podocyte loss indicated by the urine podocin mRNA to creatinine ratio (UpodCR) was higher in preterm infants than in full-term infants. UpodCR was correlated with the levels of UP and UMA. Multiple linear regression analysis also showed that lower gestational age (GA) at birth and small for gestational age (SGA) were high risk factors for urine podocyte loss.

Conclusions
Increasing urine podocyte loss was identified in preterm infants. Moreover, perinatal factors were associated with podocyte loss and may be a potential direction for comprehensive research and intervention in this field.

Graphical Abstract
A higher resolution version of the Graphical abstract is available as Supplementary information
COVID-19 testing in Delaware’s underserved communities: demographic and social determinants of testing inequality
Authors: Wang Sharron Xuanren , Rogers Nicole Bell , Harrington Melissa , Dillard Dorothy 
Published: June 2022

Abstract
Background
Health experts believe that frequent COVID-19 testing is one of the most important practices for stopping the spread of the COVID-19 virus. Demographic and social factors might play a role in whether a person gets tested for COVID. This present study aimed to investigate (1) the demographic and social factors affecting a person’s likelihood of getting tested for COVID-19, and (2) the demographic and social factors related to a positive serology test (i.e., indicating likely past infection).

Methods
Data were extracted from a survey conducted in Delaware’s underserved communities. Participants were asked to complete a questionnaire about their COVID-19 testing history, and nurses at the study site collected a serology sample from each participant.

Results
Our results indicated that Black or Hispanic individuals living in underserved communities had greater odds of having been tested previously for COVID compared to being non-Hispanic White. In addition, our study found that being female, educated, feeling safe in one’s neighborhood, being vaccinated against COVID, and being an essential worker increased one’s odds of having been previously tested for COVID-19. Regarding the results of the COVID-19 antibody serology tests, our findings revealed that Hispanic respondents were more likely to have a positive serology test compared to non-Hispanic White respondents, indicating that the Hispanic individuals were more likely to contract the virus. Educated individuals were less likely to have a positive serology test compared to the less-educated. Those who expressed hesitancy about getting vaccinated for COVID-19 and identified themselves as essential workers were more likely to have a positive serology test and to have previously contracted the virus.

Conclusions
Identifying key factors associated with COVID-19 testing may help establish novel strategies to increase testing rates among vulnerable population. Public health and policy implications are discussed in the article.
Resting-state functional connectivity patterns associated with childhood maltreatment in a large bicentric cohort of adults with and without major depression
Authors: Goltermann Janik , Winter Nils Ralf , Meinert Susanne , Sindermann Lisa , Lemke Hannah , Leehr Elisabeth J. , Grotegerd Dominik , Winter Alexandra , Thiel Katharina , Waltemate Lena , Breuer Fabian , Repple Jonathan , Gruber Marius , Richter Maike , Teckentrup Vanessa , Kroemer Nils B. , Brosch Katharina , Meller Tina , Pfarr Julia-Katharina , Ringwald Kai Gustav , Stein Frederike , Heindel Walter , Jansen Andreas , Kircher Tilo , Nenadić Igor , Dannlowski Udo , Opel Nils , Hahn Tim 
Published: June 2022

AbstractBackgroundChildhood maltreatment (CM) represents a potent risk factor for major depressive disorder (MDD), including poorer treatment response. Altered resting-state connectivity in the fronto-limbic system has been reported in maltreated individuals. However, previous results in smaller samples differ largely regarding localization and direction of effects.MethodsWe included healthy and depressed samples [n = 624 participants with MDD; n = 701 healthy control (HC) participants] that underwent resting-state functional MRI measurements and provided retrospective self-reports of maltreatment using the Childhood Trauma Questionnaire. A-priori defined regions of interest [ROI; amygdala, hippocampus, anterior cingulate cortex (ACC)] were used to calculate seed-to-voxel connectivities.ResultsNo significant associations between maltreatment and resting-state connectivity of any ROI were found across MDD and HC participants and no interaction effect with diagnosis became significant. Investigating MDD patients only yielded maltreatment-associated increased connectivity between the amygdala and dorsolateral frontal areas [pFDR < 0.001; η2partial = 0.050; 95%-CI (0.023–0.085)]. This effect was robust across various sensitivity analyses and was associated with concurrent and previous symptom severity. Particularly strong amygdala-frontal associations with maltreatment were observed in acutely depressed individuals [n = 264; pFDR < 0.001; η2partial = 0.091; 95%-CI (0.038–0.166)). Weaker evidence – not surviving correction for multiple ROI analyses – was found for altered supracallosal ACC connectivity in HC individuals associated with maltreatment.ConclusionsThe majority of previous resting-state connectivity correlates of CM could not be replicated in this large-scale study. The strongest evidence was found for clinically relevant maltreatment associations with altered adult amygdala-dorsolateral frontal connectivity in depression. Future studies should explore the relevance of this pathway for a maltreated subgroup of MDD patients.
Spatial heterogeneity of extensively drug resistant-tuberculosis in Western Cape Province, South Africa
Authors: Sy Karla Therese L. , Leavitt Sarah V. , de Vos Margaretha , Dolby Tania , Bor Jacob , Horsburgh C. Robert , Warren Robin M. , Streicher Elizabeth M. , Jenkins Helen E. , Jacobson Karen R. 
Published: June 2022

AbstractTuberculosis (TB) remains a leading infectious disease killer globally. Treatment outcomes are especially poor among people with extensively drug-resistant (XDR) TB, until recently defined as rifampicin-resistant (RR) TB with resistance to an aminoglycoside (amikacin) and a fluoroquinolone (ofloxacin). We used laboratory TB test results from Western Cape province, South Africa between 2012 and 2015 to identify XDR-TB and pre-XDR-TB (RR-TB with resistance to one second-line drug) spatial hotspots. We mapped the percentage and count of individuals with RR-TB that had XDR-TB and pre-XDR-TB across the province and in Cape Town, as well as amikacin-resistant and ofloxacin-resistant TB. We found the percentage of pre-XDR-TB and the count of XDR-TB/pre-XDR-TB highly heterogeneous with geographic hotspots within RR-TB high burden areas, and found hotspots in both percentage and count of amikacin-resistant and ofloxacin-resistant TB. The spatial distribution of percentage ofloxacin-resistant TB hotspots was similar to XDR-TB hotspots, suggesting that fluoroquinolone-resistace is often the first step to additional resistance. Our work shows that interventions used to reduce XDR-TB incidence may need to be targeted within spatial locations of RR-TB, and further research is required to understand underlying drivers of XDR-TB transmission in these locations.
Radon signature of CO2 flux constrains the depth of degassing: Furnas volcano (Azores, Portugal) versus Syabru-Bensi (Nepal Himalayas)
Authors: Girault Frédéric , Viveiros Fátima , Silva Catarina , Thapa Sandeep , Pacheco Joana E. , Adhikari Lok Bijaya , Bhattarai Mukunda , Koirala Bharat Prasad , Agrinier Pierre , France-Lanord Christian , Zanon Vittorio , Vandemeulebrouck Jean , Byrdina Svetlana , Perrier Frédéric 
Published: June 2022

AbstractSubstantial terrestrial gas emissions, such as carbon dioxide (CO2), are associated with active volcanoes and hydrothermal systems. However, while fundamental for the prediction of future activity, it remains difficult so far to determine the depth of the gas sources. Here we show how the combined measurement of CO2and radon-222 fluxes at the surface constrains the depth of degassing at two hydrothermal systems in geodynamically active contexts: Furnas Lake Fumarolic Field (FLFF, Azores, Portugal) with mantellic and volcano-magmatic CO2, and Syabru-Bensi Hydrothermal System (SBHS, Central Nepal) with metamorphic CO2. At both sites, radon fluxes reach exceptionally high values (> 10 Bq m−2 s−1) systematically associated with large CO2fluxes (> 10 kg m−2day−1). The significant radon‒CO2fluxes correlation is well reproduced by an advective–diffusive model of radon transport, constrained by a thorough characterisation of radon sources. Estimates of degassing depth, 2580 ± 180 m at FLFF and 380 ± 20 m at SBHS, are compatible with known structures of both systems. Our approach demonstrates that radon‒CO2coupling is a powerful tool to ascertain gas sources and monitor active sites. The exceptionally high radon discharge from FLFF during quiescence (≈ 9 GBq day−1) suggests significant radon output from volcanoes worldwide, potentially affecting atmosphere ionisation and climate.
Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean
Authors: Sabharwal Tanya , Lu Zhongjin , Slocum Robert D. , Kang Seongjoon , Wang Huan , Jiang Han-Wei , Veerappa Roopadarshini , Romanovicz Dwight , Nam Ji Chul , Birk Simon , Clark Greg , Roux Stanley J. 
Published: June 2022

AbstractTo address the demand for food by a rapidly growing human population, agricultural scientists have carried out both plant breeding and genetic engineering research. Previously, we reported that the constitutive expression of a pea apyrase (Nucleoside triphosphate, diphosphohydrolase) gene, psNTP9, under the control of the CaMV35S promoter, resulted in soybean plants with an expanded root system architecture, enhanced drought resistance and increased seed yield when they are grown in greenhouses under controlled conditions. Here, we report that psNTP9-expressing soybean lines also show significantly enhanced seed yields when grown in multiple different field conditions at multiple field sites, including when the gene is introgressed into elite germplasm. The transgenic lines have higher leaf chlorophyll and soluble protein contents and decreased stomatal density and cuticle permeability, traits that increase water use efficiency and likely contribute to the increased seed yields of field-grown plants. These altered properties are explained, in part, by genome-wide gene expression changes induced by the transgene.
Evidence for mitochondrial Lonp1 expression in the nucleus
Authors: Gibellini Lara , Borella Rebecca , De Gaetano Anna , Zanini Giada , Tartaro Domenico Lo , Carnevale Gianluca , Beretti Francesca , Losi Lorena , De Biasi Sara , Nasi Milena , Forcato Mattia , Cossarizza Andrea , Pinti Marcello 
Published: June 2022

AbstractThe coordinated communication between the mitochondria and nucleus is essential for cellular activities. Nonetheless, the pathways involved in this crosstalk are scarcely understood. The protease Lonp1 was previously believed to be exclusively located in the mitochondria, with an important role in mitochondrial morphology, mtDNA maintenance, and cellular metabolism, in both normal and neoplastic cells. However, we recently detected Lonp1 in the nuclear, where as much as 22% of all cellular Lonp1 can be found. Nuclear localization is detectable under all conditions, but the amount is dependent on a response to heat shock (HS). Lonp1 in the nucleus interacts with heat shock factor 1 (HSF1) and modulates the HS response. These findings reveal a novel extramitochondrial function for Lonp1 in response to stress.