bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between implantable loop recorder use and secondary stroke prevention: a meta-analysis
Authors: Huang Wen-Yi , Ovbiagele Bruce , Hsieh Cheng-Yang , Lee Meng 
Published: June 2022

ObjectiveTo conduct a meta-analysis of randomised controlled trials (RCTs) to evaluate the impact of ILR use on occurrence of recurrent stroke.MethodsPubMed, EMBASE, CENTRAL and ClinicalTrials.gov were searched from 1966 to November 2021 to identify RCTs comparing ILR versus non-ILR in patients with ischaemic stroke. Relative risk (RR) with 95% CI was used as a measure of the effect of ILR versus non-ILR on recurrent stroke, recurrent ischaemic stroke, AF detection and oral anticoagulant (OAC) initiation. A fixed-effect estimate based on the Mantel-Haenszel method was computed.ResultsWe identified three RCTs with 1233 patients with ischaemic stroke. Among three included RCTs, 54 recurrent stroke events were reported in two RCTs and 84 recurrent ischaemic stroke events were reported in three RCTs. Pooled results showed that patients who received ILR versus no ILR was not associated with a significantly reduced risk of recurrent stroke (5.6% vs 8.0%; RR 0.70; 95% CI 0.42 to 1.19) or recurrent ischaemic stroke (5.7% vs 7.9%; RR 0.72; 95% CI 0.48 to 1.10). Compared to non-ILR patients, ILR patients had higher rates of AF detection (12.9% vs 2.4%; RR 5.31; 95% CI, 3.10 to 9.11) and OAC initiation (15.2% vs 5.5%; RR 2.77; 95% CI 1.90 to 4.03).ConclusionsPatients assigned to ILR vs non-ILR did not have a significantly reduced risk of recurrent stroke or recurrent ischaemic stroke despite higher rates of AF detection and OAC initiation. Sufficiently powered RCTs of ILR to assess the risk of recurrent stroke are warranted.
Performance of the first commercial dual resistance assay, AmpliSens Mycoplasma genitalium-ML/FQ-Resist-FL, for detection of potential macrolide and quinolone resistance-associated mutations and prevalence of M. genitalium resistance mutations in St. Petersburg, Russia
Authors: Shipitsyna Elena , Khusnutdinova Tatiana , Budilovskaya Olga , Shedko Elizaveta , Goloveshkina Elena , Khayrullina Guzel , Krysanova Anna , Shalepo Kira , Savicheva Alevtina , Unemo Magnus 
Published: June 2022

ObjectivesAntimicrobial resistance in Mycoplasma genitalium (MG) is a poorly surveyed and controlled global health concern. We evaluated the first commercial dual resistance assay, AmpliSens M. genitalium-ML/FQ-Resist-FL assay, for detection of potential macrolide and quinolone resistance-associated mutations (MRAMs and QRAMs, respectively) and estimated the prevalence of these mutations in MG in St. Petersburg, Russia.MethodsUrogenital samples positive (n=145 from 2007 to 2020) and negative (n=56 from 2021) for MG in routine diagnostics were retrospectively analysed using the AmpliSens M. genitalium-ML/FQ-Resist-FL assay (Central Research Institute of Epidemiology, Moscow, Russia) and Sanger sequencing for validation.ResultsThe AmpliSens M. genitalium-ML/FQ-Resist-FL assay detected potential MRAMs and QRAMs with sensitivities of 100% (CI95% 83.9 to 100) and 92.3% (CI95% 66.7 to 99.6) and specificities of 99.2% (CI95% 95.6 to 100) and 100% (CI95% 97.2 to 100), respectively, in clinical specimens with ≥1000 MG geq/mL. In total, MRAMs were detected in 13.8% (CI95% 9.1 to 20.3) of samples, with 23S rRNA A2058G being the most prevalent mutation (45.0% (CI95% 25.8 to 65.8)). QRAMs were found in 9.0% (CI95% 5.3 to 14.7) of samples, with S83I the most frequent mutation (53.8% (CI95% 29.1 to 76.8)). Dual resistance was observed in 5.5% (CI95% 2.8 to 10.5) of samples. Potential MRAM and dual resistance rates significantly increased over time: from 0% in 2007–2008 to 25% (ptrend=0.0009) and 10% (ptrend=0.0447), respectively, in 2018–2020. QRAM rate appeared to increase (from 0% to 13%), but significance was not reached (ptrend=0.0605).ConclusionsThe rapid increase in MG antimicrobial resistance in St. Petersburg, especially prominent for MRAMs, necessitates implementation of macrolide resistance-guided therapy in Russia. The first commercial dual resistance assay, AmpliSens M. genitalium-ML/FQ-Resist-FL assay, was sensitive and specific for detection of potential MRAMs and QRAMs and could be valuable in macrolide resistance-guided therapies and possibly for surveillance of QRAMs. International surveillance of antimicrobial resistance-associated mutations in MG, further research into clinical relevance of several parC mutations and novel treatments are essential.
Tobacco retailers around schools in 10 cities across China
Authors: Nian Qinghua , Cohen Joanna E , Cui Yuxian , Zhang Shuai 
Published: June 2022

ObjectiveThis study explored the nature and extent of tobacco product availability at retailers near junior, senior and vocational high schools in rural and urban areas in 10 cities across China, including Shanghai, Beijing, Guangzhou, Chongqing, Shenyang, Jinan, Kunming, Guilin, Kaifeng and Baiyin.MethodsTobacco sales at 1612 retailers around 904 schools (within 100 m for urban schools and within 250 m for rural schools) were observed by trained data collectors with an online observation form in 2021. Multistage sampling with simple random sampling at each stage was used to select schools from the city district-level/rural county-level divisions. χ2tests and analyses of variance were used to analyse the data.FindingsOver half of urban schools (57.0%) and a large majority of rural schools (71.0%) had a cigarette retailer within a 100 m and a 250 m radius, respectively. Nearly all cigarette and electronic cigarette (e-cigarette) retailers displayed tobacco products inside. Majority of cigarette retailers (63.2%) sold flavoured cigarettes, of which 88.6% sold menthol/mint-flavoured cigarettes. Approximately half of these retailers did not display required signage prohibiting sales to minors. Overall, outcomes varied across cities and between urban and rural areas, but not by school type.ConclusionDisplay of tobacco products is very prevalent near schools in 10 cities across China. In contrast, compliance with posting ‘no sales to minors’ signs is low. Our findings suggest that effective enforcement of prohibiting tobacco retailers around schools and stronger restrictions on tobacco displays and flavoured cigarettes and e-cigarettes are needed to protect youth in China.
Twist moves and the affine index polynomials of virtual knots
Authors: Jeong Myeong-Ju , Choi Younhee , Kim Dojin 
Published: June 2022

In this paper, we give a necessary condition for two virtual knots to be related by a finite sequence of twist moves by using the affine index polynomial, which is a Vassiliev invariant of degree [Formula: see text]. Trapp showed that a numerical Vassiliev invariant of degree [Formula: see text] has a polynomial growth of degree [Formula: see text] on a twist sequence of knots, which can be extended to a twist sequence of virtual knots. We calculate the growth of the affine index polynomial for a twist sequence of virtual knots and find the difference of the affine index polynomials of two virtual knots, which are related by a twist move. Moreover, we give a lower bound for the number of twist moves needed to transform [Formula: see text] to [Formula: see text] if [Formula: see text] and [Formula: see text] are virtual knots related by a finite sequence of twist moves.
CFD MODELS FOR ANEURYSM ANALYSES AND THEIR USE IN IDENTIFYING THROMBOSIS FORMATION AND RISK ASSESSMENT
Authors: PAHLAVANI H. , OZDEMIR I. B. , YILDIRIM D. 
Published: June 2022

In this paper, the blood transport has been investigated using the single- and the two-phase methods. In the two-phase Euler–Euler approach, the blood is represented by two interpenetrating continua where the dispersed red blood cells (RBCs) of non-Newtonian characteristics are suspended in the continuous Newtonian plasma. The results of the two-phase model, where the RBC’s phase is assumed to be Carreau–Yasuda fluid, are validated against the experimental data. Furthermore, comparative analyses were performed in two patient-specific aneurysms, which indicated that for a given pulsatile flow rate, the two-phase blood approach is vitally advantageous over the single-phase assumption, and revealed a deeper inflow penetration, more complex flow structures and denser flow diversion zones in the aneurysm sac. It was obvious that the high OSI values calculated by the two-phase model covered much wider regions than the values predicted by the single-phase model. It was equally crucial that these regions coincided with the TAWSS values lower than the threshold that the single-phase approach can predict. Apparently, the single-phase model failed to spot sites of high rupture risk. The results were further exploited to identify the RBCs aggregation regions as, for example, the concave structures and narrow paths in the saccular aneurysms, for their possible use as the precursors of the thrombus formation.
Asset Management in Machine Learning: State-of-research and State-of-practice
Authors: Idowu Samuel , Strüber Daniel , Berger Thorsten 
Published: June 2022

Machine learning components are essential for today’s software systems, causing a need to adapt traditional software engineering practices when developing machine-learning-based systems. This need is pronounced due to many development-related challenges of machine learning components such as asset, experiment, and dependency management. Recently, many asset management tools addressing these challenges have become available. It is essential to understand the support such tools offer to facilitate research and practice on building new management tools with native supports for machine learning and software engineering assets.
This article positions machine learning asset management as a discipline that provides improved methods and tools for performing operations on machine learning assets. We present a feature-based survey of 18 state-of-practice and 12 state-of-research tools supporting machine-learning asset management. We overview their features for managing the types of assets used in machine learning experiments. Most state-of-research tools focus on tracking, exploring, and retrieving assets to address development concerns such as reproducibility, while the state-of-practice tools also offer collaboration and workflow-execution-related operations. In addition, assets are primarily tracked intrusively from the source code through APIs and managed via web dashboards or command-line interfaces (CLIs). We identify asynchronous collaboration and asset reusability as directions for new tools and techniques.
Effects of Dietary Lipid Levels on the Lipid Deposition and Metabolism of Subadult Triploid Rainbow Trout (Oncorhynchus mykiss)
Authors: Meng Yuqiong , Tian Haining , Hu Xuemin , Han Buying , Li Xiaohua , Cangzhong Li , Ma Rui 
Published: June 2022

A growth trial was conducted to evaluate the effects of dietary lipid levels on the lipid deposition and metabolism of subadult triploid rainbow trout. Diets with low (148 g lipid kg-1 diet), moderate (228 g lipid kg-1 diet), and high (294 g lipid kg-1 diet) crude lipid contents were fed to quadruplicated groups of fish (

233

g
±
0.2

g

) for 80 days, and they were named as LL, ML, and HL, respectively. Results showed that the lowest and highest values of condition factor and hepatosomatic index were shown in the LL group, respectively, while the HL group obtained the highest liver redness value, plasma total cholesterol, high-density lipoprotein-cholesterol, and low-density lipoprotein-cholesterol contents (

P
<
0.05

). The viscerosomatic index and plasm glucose, nonesterified fatty acids, and triglyceride contents were comparable among groups (

P
>
0.05

). As for lipid deposition, viscera and muscle were the main lipid storage place for triploid rainbow trout when tissues’ weight is taken into consideration. Overall quantitative PCR showed that the lipid uptake, triglyceride, and fatty acid catabolism were upregulated, but glycolysis was downregulated as dietary lipid increased. The expression of genes involved in lipogenesis, lipoprotein clearance, very low-density lipoprotein assembly, and glycogenesis was not affected by dietary lipid levels (

P
>
0.05

). In summary, subadult triploid rainbow trout store lipid mainly in viscera and muscle; it could maintain hepatic lipid homeostasis when fed with dietary lipid levels ranging from 150 to 300 g kg-1 by regulating lipid uptake and switching energy supply between glycolysis and fatty acids β-oxidation.
Blockade of B7-H1 and PD-1 by Monoclonal Antibodies Potentiates Cancer Therapeutic Immunity
Authors: Hirano Fumiya , Kaneko Katsumi , Tamura Hideto , Dong Haidong , Wang Shengdian , Ichikawa Masao , Rietz Cecilia , Flies Dallas B. , Lau Julie S. , Zhu Gefeng , Tamada Koji , Chen Lieping 
Published: June 2022

AbstractContemporary approaches for vaccination and immunotherapy are often capable of eliciting strong T-cell responses against tumor antigens. However, such responses are not parallel to clinical tumor regression. The development of evasion mechanisms within tumor microenvironment may be responsible for poor therapeutic responses. We report here that constitutive or inducible expression of B7-H1, a B7 family molecule widely expressed by cancers, confers resistance to therapeutic anti-CD137 antibody in mice with established tumors. The resistance is accompanied with failure of antigen-specific CD8+ CTLs to destroy tumor cells without impairment of CTL function. Blockade of B7-H1 or PD-1 by specific monoclonal antibodies could reverse this resistance and profoundly enhance therapeutic efficacy. Our findings support that B7-H1/PD-1 forms a molecular shield to prevent destruction by CTLs and implicate new approaches for immunotherapy of human cancers.
Carnosol Inhibits β-Catenin Tyrosine Phosphorylation and Prevents Adenoma Formation in the C57BL/6J/Min/+ (Min/+) Mouse
Authors: Moran Amy E. , Carothers Adelaide M. , Weyant Michael J. , Redston Mark , Bertagnolli Monica M. 
Published: June 2022

Abstract
Carnosol, a constituent of the herb, rosemary, has shown beneficial medicinal and antitumor effects. Using the C57BL/6J/Min/+ (Min/+) mouse, a model of colonic tumorigenesis, we found that dietary administration of 0.1% carnosol decreased intestinal tumor multiplicity by 46%. Previous studies showed that tumor formation in the Min/+ mouse was associated with alterations in the adherens junctions, including an increased expression of tyrosine-phosphorylated β-catenin, dissociation of β-catenin from E-cadherin, and strongly reduced amounts of E-cadherin located at lateral plasma membranes of histologically normal enterocytes. Here, we confirm these findings and show that treatment of Min/+ intestinal tissue with carnosol restored both E-cadherin and β-catenin to these enterocyte membranes, yielding a phenotype similar to that of the Apc+/+ wild-type (WT) littermate. Moreover, treatment of WT intestine with the phosphatase inhibitor, pervanadate, removed E-cadherin and β-catenin from the lateral membranes of enterocytes, mimicking the appearance of the Min/+ tissue. Pretreatment of WT tissue with carnosol inhibited the pervanadate-inducible expression of tyrosine-phosphorylated β-catenin. Thus, the ApcMin allele produces adhesion defects that involve up-regulated expression of tyrosine-phosphorylated proteins, including β-catenin. Moreover, these data suggest that carnosol prevents Apc-associated intestinal tumorigenesis, potentially via its ability to enhance E-cadherin-mediated adhesion and suppress β-catenin tyrosine phosphorylation.
Particle Irradiation Suppresses Metastatic Potential of Cancer Cells
Authors: Ogata Toshiyuki , Teshima Teruki , Kagawa Kazufumi , Hishikawa Yoshio , Takahashi Yutaka , Kawaguchi Atsuko , Suzumoto Yuko , Nojima Kumie , Furusawa Yoshiya , Matsuura Nariaki 
Published: June 2022

Abstract
Particle radiotherapy such as proton and carbon ion has been producing promising clinical results worldwide. The purpose of this study was to compare metastatic capabilities of malignant tumor cells after irradiation with photon, proton, and carbon ion beams to clarify their ion beam–specific biological effects. We examined the biological properties of highly aggressive HT1080 human fibrosarcoma cells to assess their metastatic processes in terms of cell adhesion capability to extracellular matrix, expression of integrins, cell migration, cell invasive capability, and matrix metalloproteinase-2 activity in vitro. We then assessed the metastatic capabilities of LM8 mouse osteosarcoma irradiated with carbon ion or photon beam in the syngeneic mice. Both proton and carbon ion irradiation decreased cell migration and invasion in a dose-dependent manner and strongly inhibited matrix metalloproteinase-2 activity. On the other hand, lower X-ray irradiation promoted cell migration and invasion concomitant with up-regulation of αVβ3 integrin. For cancer cells treated with carbon ion irradiation, the number of pulmonary metastasis was decreased significantly in vivo. These findings suggest that particle irradiation suppresses metastatic potential even at lower dose, whereas photon irradiation promotes cell migration and invasive capabilities at lower dose level, and provide preclinical evidence that ion beam radiotherapy may be superior to conventional photon beam therapy in possible preventive effects on metastases of irradiated malignant tumor cells.
Matrix Metalloproteinase-2 Contributes to Cancer Cell Migration on Collagen
Authors: Xu Xiaoping , Wang Yao , Chen Zhihua , Sternlicht Mark D. , Hidalgo Manuel , Steffensen Bjorn 
Published: June 2022

Abstract
Matrix metalloproteinases (MMP) are central to tissue penetration by cancer cells, as tumors expand and form metastases, but the mechanism by which MMP-2 contributes to cancer cell migration is not well understood. In the present experiments, both a broad-spectrum MMP inhibitor and the isolated collagen binding domain (CBD) from MMP-2 inhibited cell migration on native type I collagen. These results verified the involvement of MMPs in general and showed that MMP-2, specifically, contributes to cell migration by a mechanism involving MMP-2 interaction with collagen. To exclude potential overlapping effects of MMP-9, additional experiments showed that MMP-2 also contributed to migration of MMP-9−/− cells. To investigate whether the homologous CBD from human fibronectin also inhibited cell migration, we first showed that fragmentation of fibronectin is a feature of breast cancer tumors and that several fragments contained the CBD. However, the recombinant fibronectin domain did not alter cell migration on collagen. This lack of effect on cell migration was explored in competitive protein-protein binding assays, which showed that the affinity of MMP-2 for collagen exceeds that of fibronectin. Furthermore, whereas the isolated MMP-2 CBD inhibited the gelatinolytic activities of MMP-2 and tumor extracts, such an inhibition was not characteristic of the corresponding fibronectin domain. Together, our results provide evidence that MMP-2 is an important determinant of cancer cell behavior but is not inhibited by the collagen binding segment of fibronectin.
Transcriptional Profiles of Intestinal Tumors in Apc
Min Mice are Unique from those of Embryonic Intestine and Identify Novel Gene Targets Dysregulated in Human Colorectal Tumors
Authors: Reichling Tim , Goss Kathleen Heppner , Carson Daniel J. , Holdcraft Robert W. , Ley-Ebert Cathy , Witte Dave , Aronow Bruce J. , Groden Joanna 
Published: June 2022

Abstract
The adenomatous polyposis coli (APC) tumor suppressor is a major regulator of the Wnt signaling pathway in normal intestinal epithelium. APC, in conjunction with AXIN and GSK-3β, forms a complex necessary for the degradation of β-catenin, thereby preventing β-catenin/T-cell factor interaction and alteration of growth-controlling genes such as c-MYC and cyclin D1. Inappropriate activation of the Wnt pathway, via Apc/APC mutation, leads to gastrointestinal tumor formation in both the mouse and human. In order to discover novel genes that may contribute to tumor progression in the gastrointestinal tract, we used cDNA microarrays to identify 114 genes with altered levels of expression in ApcMin mouse adenomas from the duodenum, jejunum, and colon. Changes in the expression of 24 of these 114 genes were not observed during mouse development at embryonic day 16.5, postnatal day 1, or postnatal day 14 (relative to normal adult intestine). These 24 genes are not previously known Wnt targets. Seven genes were validated by real-time reverse transcription-PCR analysis, whereas four genes were validated by in situ hybridization to mouse adenomas. Real-time reverse transcription-PCR analysis of human colorectal cancer cell lines and adenocarcinomas revealed that altered expression levels were also observed for six of the genes Igfbp5, Lcn2, Ly6d, N4wbp4 (PMEPA1), S100c, and Sox4.
cIAP1 Localizes to the Nuclear Compartment and Modulates the Cell Cycle
Authors: Samuel Temesgen , Okada Kazuya , Hyer Marc , Welsh Kate , Zapata Juan M. , Reed John C. 
Published: June 2022

Abstract
We explored the location and function of the human cIAP1 protein, a member of the inhibitor of apoptosis protein (IAP) family. Unlike family member X-linked IAP (XIAP), which was predominantly cytoplasmic, the cIAP1 protein localized almost exclusively to nuclei in cells, as determined by immunofluorescence microscopy and subcellular fractionation methods. Interestingly, apoptotic stimuli induced nuclear export of cIAP1, which was blocked by a chemical caspase inhibitor. In dividing cells, cIAP1 was released into the cytosol early in mitosis, then reaccumulated in nuclei in late anaphase and in telophase, with the exception of a pool of cIAP1 that associated with the midbody. Survivin, another IAP family member, and cIAP1 were both localized on midbody microtubules at telophase, and also interacted with each other during mitosis. Cells stably overexpressing cIAP1 accumulated in G2-M phase and grew slower than control-transfected cells. These cIAP1-overexpressing cells also exhibited cytokinesis defects over 10 times more often than control cells and displayed a mitotic checkpoint abnormality with production of polyploid cells when exposed to microtubule-targeting drugs nocodazole and paclitaxel (Taxol). Our findings demonstrate a role for overexpressed cIAP1 in genetic instability, possibly by interfering with mitotic functions of Survivin. These findings may have important implications for cancers in which cIAP1 overexpression occurs.