bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case report: Long-term follow-up of a large full-thickness skin defect treated with a photosynthetic scaffold for dermal regeneration
Authors: Obaíd Miguel L. , Carvajal Felipe , Camacho Juan Pablo , Corrales-Orovio Rocío , Martorell Ximena , Varas Juan , Calderón Wilfredo , Guzmán Christian Dani , Brenet Marianne , Castro Margarita , Orlandi Cecilia , San Martín Sebastián , Eblen-Zajjur Antonio , Egaña José Tomás 
Published: December 2022

It is broadly described that almost every step of the regeneration process requires proper levels of oxygen supply; however, due to the vascular disruption in wounds, oxygen availability is reduced, being detrimental to the regeneration process. Therefore, the development of novel biomaterials combined with improved clinical procedures to promote wound oxygenation is an active field of research in regenerative medicine. This case report derives from a cohort of patients enrolled in a previously published ongoing phase I clinical trial (NCT03960164), to assess safety of photosynthetic scaffolds for the treatment of full skin defects. Here, we present a 56 year old patient, with a scar contracture in the cubital fossa, which impaired the elbow extension significantly affecting her quality of life. As part of the treatment, the scar contracture was removed, and the full-thickness wound generated was surgically covered with a photosynthetic scaffold for dermal regeneration, which was illuminated to promote local oxygen production. Then, in a second procedure, an autograft was implanted on top of the scaffold and the patient’s progress was followed for up to 17 months. Successful outcome of the whole procedure was measured as improvement in functionality, clinical appearance, and self-perception of the treated area. This case report underscores the long-term safety and applicability of photosynthetic scaffolds for dermal regeneration and their stable compatibility with other surgical procedures such as autograft application. Moreover, this report also shows the ability to further improve the clinical outcome of this procedure by means of dermal vacuum massage therapy and, more importantly, shows an overall long-term improvement in patient´s quality of life, supporting the translation of photosynthetic therapies into human patients.
Thermal-tolerant potential of ordinary Chlorella pyrenoidosa and the promotion of cell harvesting by heterotrophic cultivation at high temperature
Authors: Dai Yu-Ren , Wang Die , Zhu Yu-Rong , Yang Kun-Xiao , Jiao Ning , Sun Zhong-Liang , Wang Shi-Kai 
Published: December 2022

During the heterotrophic cultivation of microalgae, a cooled process against temperature rise caused by the metabolism of exogenous organic carbon sources greatly increases cultivation cost. Furthermore, microalgae harvesting is also a cost-consuming process. Cell harvesting efficiency is closely related to the characteristics of the algal cells. It may be possible to change cell characteristics through controlling culture conditions to make harvesting easier. In this study, the mesophilic Chlorella pyrenoidosa was found to be a thermal-tolerant species in the heterotrophic mode. The cells could maintain their maximal specific growth rate at 40°C and reached 1.45 day−1, which is equivalent to that of cultures at 35°C but significantly higher than those cultured at lower temperatures. Interestingly, the cells cultured at 40°C were much easier to be harvested than those at lower temperatures. The harvesting efficiency of the cells cultured at 40°C reached 96.83% after sedimentation for 240 min, while the cells cultured at lower temperatures were reluctant to settle. Likely, the same circumstance occurred when cells were harvested by centrifugation or flocculation. The promotion of cell harvesting for cells cultured at high temperatures was mainly attributed to increased cell size and decreased cell surface charge. To the best of our knowledge, this is the first report that cells cultured at high temperatures can promote microalgae harvesting. This study explores a new approach to simplify the cultivation and harvesting of microalgae, which effectively reduces the microalgae production cost.
Biphenyls in Clusiaceae: Isolation, structure diversity, synthesis and bioactivity
Authors: Wang Youyi , Huang Qing , Zhang Li , Zheng Changwu , Xu Hongxi 
Published: December 2022

Clusiaceae plants contain a wide range of biologically active metabolites that have gotten a lot of interest in recent decades. The chemical compositions of these plants have been demonstrated to have positive effects on a variety of ailments. The species has been studied for over 70 years, and many bioactive compounds with antioxidant, anti-proliferative, and anti-inflammatory properties have been identified, including xanthones, polycyclic polyprenylated acylphloroglucinols (PPAPs), benzophenones, and biphenyls. Prenylated side chains have been discovered in many of these bioactive substances. To date, there have been numerous studies on PPAPs and xanthones, while no comprehensive review article on biphenyls from Clusiaceae has been published. The unique chemical architectures and growing biological importance of biphenyl compounds have triggered a flurry of research and interest in their isolation, biological evaluation, and mechanistic studies. In particular, the FDA-approved drugs such as sonidegib, tazemetostat, daclatasvir, sacubitril and trifarotene are closely related to their biphenyl-containing moiety. In this review, we summarize the progress and development in the chemistry and biological activity of biphenyls in Clusiaceae, providing an in-depth discussion of their structural diversity and medicinal potential. We also present a preliminary discussion of the biological effects with or without prenyl groups on the biphenyls.
Sodium houttuyfonate effectively treats acute pulmonary infection of Pseudomonas aeruginosa by affecting immunity and intestinal flora in mice
Authors: Zhuang Tian , Hu Mengxue , Wang Jian , Mei Longfei , Zhu Xiaoxiao , Zhang Haitao , Jin Feng , Shao Jing , Wang Tianming , Wang Changzhong , Niu Xiaojia , Wu Daqiang 
Published: December 2022

IntroductionPseudomonas aeruginosa is a major nosocomial pathogen that frequently causes ventilator-associated pneumonia in specific populations. Sodium houttuyfonate (SH) has shown mild antibacterial activity against P. aeruginosa in vitro, but the mechanism of potent antimicrobial activity of SH against P. aeruginosa infection in vivo remains unclear.MethodsHere, using the mouse pneumonia model induced by P. aeruginosa nasal drip to explore the therapeutic effects of SH.ResultsWe found that SH exhibits dose-dependent therapeutic effects of reducing P. aeruginosa burden and systemic inflammation in pneumonia mice. SH ameliorates inflammatory gene expression and production of inflammatory proteins, such as interleukin-6 (IL-6), nuclear factor kappa-B (NF-κB) and toll-like receptor 4 (TLR4), associated with the TLR4/NF-κB pathway in mice with P. aeruginosa pneumonia. Furthermore, we analyzed the intestinal flora of mice and found that compared with the model group, the abundance and diversity of beneficial bacterial flora of SH treatment groups increased significantly, suggesting that SH can improve the intestinal flora disorder caused by inflammation. In addition, SH improves alpha and beta diversity index and reduces species abundance differences of intestinal flora in pneumonia mice.DiscussionTaken together, our presented results indicate that SH may effectively alleviate the acute pulmonary infection induced by P. aeruginosa by reducing the disturbance of regulating immunity and intestinal flora in mice.
Effects of temporal framing and hazard experience on receptivity to hurricane risk messages: A survey of U.S. Gulf Coast residents
Authors: Retchless David P. , Ross Ashley D. 
Published: December 2022

IntroductionAs knowledge in the scientific community increases regarding how anthropogenic CO2 loading of the atmosphere will impact future hurricane activity, the need for effective and accurate communication of hurricane risk in coastal communities—specifically to non-scientific stakeholders—also increases.MethodsTo better inform hurricane risk communication, this study employs a survey of U.S. Gulf Coast residents immediately following the active 2017 hurricane season to consider how message framing (as established by communicator word choice) and stakeholders' experience may affect receptivity to hurricane hazard information. Specifically, it tests how respondents' perceptions of hurricane risk varied with prior experience and following exposure to randomly assigned, infographic-based depictions of trends in major hurricane (≥ category 3, Saffir Simpson Scale) frequency. The set of randomly assigned infographics varied in both temporal framing (past-to-present trends based on paleo data versus present-to-future trends based on climate projections) and causal framing (‘climate change' versus ‘ocean and atmospheric change').ResultsDamaging hurricane experience may be associated with a tendency towards increasing hurricane risk perceptions after viewing the infographics. For framing effects, temporal framing of hurricane risk messages matters, with forward-looking framings (future projections) causing increased perceptions of risk and storm frequency; effects of causal framing were not significant. The results also show that Gulf Coast residents tend to be more optimistic about the future frequency of major hurricanes than most scientists and, consistent with confirmation and optimism biases, are somewhat resistant to changing their prior risk beliefs based on infographic exposure.DiscussionThese results suggest that future projections – rather than paelo analogs – may be best for quickly communicating expected increases in hurricane risk. Communicators should consider these factors when translating scientific hurricane risk information to the public.
Construction of a diagnostic signature and immune landscape of pulmonary arterial hypertension
Authors: Duo Mengjie , Liu Zaoqu , Zhang Yuyuan , Li Pengfei , Weng Siyuan , Xu Hui , Wang Yu , Jiang Tianci , Wu Ruhao , Cheng Zhe 
Published: December 2022

BackgroundMolecular biomarkers are widely used for disease diagnosis and exploration of pathogenesis. Pulmonary arterial hypertension (PAH) is a rapidly progressive cardiopulmonary disease with delayed diagnosis. Studies were limited regarding molecular biomarkers correlated with PAH from a broad perspective.MethodsTwo independent microarray cohorts comprising 73 PAH samples and 36 normal samples were enrolled in this study. The weighted gene co-expression network analysis (WGCNA) was performed to identify the key modules associated with PAH. The LASSO algorithm was employed to fit a diagnostic model. The latent biology mechanisms and immune landscape were further revealed via bioinformatics tools.ResultsThe WGCNA approach ultimately identified two key modules significantly associated with PAH. For genes within the two models, differential expression analysis between PAH and normal samples further determined nine key genes. With the expression profiles of these nine genes, we initially developed a PAH diagnostic signature (PDS) consisting of LRRN4, PI15, BICC1, PDE1A, TSHZ2, HMCN1, COL14A1, CCDC80, and ABCB1 in GSE117261 and then validated this signature in GSE113439. The ROC analysis demonstrated outstanding AUCs with 0.948 and 0.945 in two cohorts, respectively. Besides, patients with high PDS scores enriched plenty of Th17 cells and neutrophils, while patients with low PDS scores were dramatically related to mast cells and B cells.ConclusionOur study established a robust and promising signature PDS for diagnosing PAH, with key genes, novel pathways, and immune landscape offering new perspectives for exploring the molecular mechanisms and potential therapeutic targets of PAH.
Combined treatment by burosumab and a calcimimetic can ameliorate hypophosphatemia due to excessive actions of FGF23 and PTH in adult XLH with tertiary hyperparathyroidism: A case report
Authors: Takashi Yuichi , Toyokawa Kyoko , Oda Naoki , Muta Yoshimi , Yokomizo Hisashi , Fukumoto Seiji , Kawanami Daiji 
Published: December 2022

IntroductionX-linked hypophosphatemia (XLH) is the most prevalent type of heritable fibroblast growth factor 23 (FGF23)-related hypophosphatemic rickets. Recently, anti-FGF23 antibody, burosumab, has become clinically available. We herein report a patient with adult XLH and tertiary hyperparathyroidism.Case presentationThe serum phosphate level and tubular maximum reabsorption of phosphate per glomerular filtration rate (TmP/GFR) remained low, despite burosumab treatment. While the influence of the relationship between FGF23 and parathyroid hormone (PTH) on the phosphaturic effect is unclear, it was considered that a high level of PTH due to tertiary hyperparathyroidism remains to suppress renal phosphate reabsorption. A calcimimetic, evocalcet, increased the serum phosphate level and TmP/GFR.Discussion and conclusionTherefore, it is important to evaluate the presence of secondary-tertiary hyperparathyroidism in patients whose serum phosphate level does not increase with burosumab treatment.
Protein O-GlcNAcylation as a nutrient sensor signaling placental dysfunction in hypertensive pregnancy
Authors: dos Passos Junior Rinaldo Rodrigues , de Freitas Raiany Alves , Dela Justina Vanessa , San Martín Sebastián , Lima Victor Vitorino , Giachini Fernanda Regina 
Published: December 2022

IntroductionDuring pregnancy, arterial hypertension may impair placental function, which is critical for a healthy baby's growth. Important proteins during placentation are known to be targets for O-linked β-N-acetylglucosamine modification (O-GlcNAcylation), and abnormal protein O-GlcNAcylation has been linked to pathological conditions such as hypertension. However, it is unclear how protein O-GlcNAcylation affects placental function and fetal growth throughout pregnancy during hypertension.MethodsTo investigate this question, female Wistar and spontaneously hypertensive rats (SHR) were mated with male Wistar rats, and after pregnancy confirmation by vaginal smear, rats were divided into groups of 14, 17, and 20 days of pregnancy (DOPs). On the 14th, 17th, and 20th DOP, rats were euthanized, fetal parameters were measured, and placentas were collected for western blot, immunohistochemical, and morphological analyses.ResultsSHR presented a higher blood pressure than the Wistar rats (p=0.001). Across all DOPs, SHR showed reduced fetal weight and an increase in small-for-gestational-age fetuses. While near-term placentas were heavier in SHR (p=0.006), placental efficiency decreased at 17 (p=0.01) and 20 DOPs (p&lt;0.0001) in this group. Morphological analysis revealed reduced junctional zone area and labyrinth vasculature changes on SHR placentas in all DOPs. O-GlcNAc protein expression was lower in placentas from SHR compared with Wistar at 14, 17, and 20 DOPs. Decreased expression of O-GlcNAc transferase (p=0.01) and O-GlcNAcase (p=0.002) enzymes was found at 14 DOPs in SHR. Immunohistochemistry showed reduced placental O-GlcNAc content in both the junctional zone and labyrinth of the placentas from SHR. Periodic acid-Schiff analysis showed decreased glycogen cell content in the placentas from SHR at 14, 17, and 20 DOPs. Moreover, glucose transporter 1 expression was decreased in placentas from SHR in all DOPs.ConclusionsThese findings suggest that decreased protein O-GlcNAcylation caused by insufficient placental nutritional apport contributes to placental dysfunction during hypertensive pregnancy, impairing fetal growth.
Identification of a basement membrane-related gene signature for predicting prognosis and estimating the tumor immune microenvironment in breast cancer
Authors: Cai Jiehui , Zhang Xinkang , Xie Wanchun , Li Zhiyang , Liu Wei , Liu An 
Published: December 2022

IntroductionBreast cancer (BC) is the most common malignancy in the world and has a high cancer-related mortality rate. Basement membranes (BMs) guide cell polarity, differentiation, migration and survival, and their functions are closely related to tumor diseases. However, few studies have focused on the association of basement membrane-related genes (BMRGs) with BC. This study aimed to explore the prognostic features of BMRGs in BC and provide new directions for the prevention and treatment of BC.MethodsWe collected transcriptomic and clinical data of BC patients from TCGA and GEO datasets and constructed a predictive signature for BMRGs by using univariate, least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression analysis. The reliability of the model was further evaluated and validated by Kaplan-Meier survival curves and receiver operating characteristic curves (ROC). Column line plots and corresponding calibration curves were constructed. Possible biological pathways were investigated by enrichment analysis. Afterward, we assessed the mutation status by tumor mutational burden (TMB) analysis and compared different subtypes using cluster analysis. Finally, we examined drug treatment sensitivity and immunological correlation to lay the groundwork for more in-depth studies in this area.ResultsThe prognostic risk model consisted of 7 genes (FBLN5, ITGB2, LAMC3, MMP1, EVA1B, SDC1, UNC5A). After validation, we found that the model was highly reliable and could accurately predict the prognosis of BC patients. Cluster analysis showed that patients with cluster 1 had more sensitive drugs and had better chances of better clinical outcomes. In addition, TMB, immune checkpoint, immune status, and semi-inhibitory concentrations were significantly different between high and low-risk groups, with lower-risk patients having the better anti-cancer ability.DiscussionThe basement membrane-related gene signature that we established can be applied as an independent prognostic factor for BC and can provide a reference for individualized treatment of BC patients.
Tillage and N-source affect soil fertility, enzymatic activity, and crop yield in a maize–rice rotation system in the Indian Terai zone
Authors: Sahoo Samaresh , Mukhopadhyay Prabir , Mowrer Jake , Maity Pragati Pramanik , Maity Aniruddha , Sinha A.K. , Sow Prodipto , Rakesh S 
Published: December 2022

A field experiment (2017–2019) was undertaken to study the short-term effects of tillage [zero tillage (ZT), conventional tillage (CT), and alternate tillage (AT)] and sources of organic and mineral fertilizer N [NS0—control, NS1—recommended doses of fertilizer (160:50:100), NS2—recommended level of fertilizer and crop residue (6 Mg·ha−1), NS3—75% of recommended N as fertilizer (120 kg·ha⁻1) and 25% N (40 kg·ha⁻1) as farm yard manure (FYM), and NS4—75% of recommended N as fertilizer and 25% N as vermicompost] on yield and soil quality under a maize–rice rotation system. Among N sources, NS4 produced the highest maize grain yield (10 Mg·ha⁻1). Residual effects of N sources on mean rice grain yield were evident only in crop residue (NS2)- and vermicompost (NS4)-treated plots. After the harvest of two complete maize–rice crop cycles, higher content of dehydrogenase activity (DHA) and urease activity (UR) were observed in the soil under AT as compared to ZT and CT at 0–10 cm (p &lt; 0.05). Similarly, microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) also recorded positive changes at 0–10 cm soil depth, especially in NS2 and NS4 treatments. AT resulted in the highest total soil carbon (TOC) (8.10 g·kg−1), followed by CT (6.73 g·kg−1) and ZT (5.98 g·kg−1). Fertilizer N treatments, however, influenced the NO3-N accumulation beyond the root zone, where crop residue-based (NS2) fertilizer N treatment resulted in the highest NO3-N (32.52 kg·ha−1), and the lowest NO3-N (14.48 kg·ha−1) was observed in the FYM-based (NS3) treatment. Therefore, the practice of alternate tillage and integration of vermicompost (40 kg·N·ha−1) and chemical fertilizer (total 120 kg·ha−1) sources should be mostly recommended to farmers in the Terai region of India.
Whole-transcriptome analyses of sheep embryonic testicular cells infected with the bluetongue virus
Authors: Lu Danfeng , Li Zhuoyue , Zhu Pei , Yang Zhenxing , Yang Heng , Li Zhanhong , Li Huachun , Li Zhuoran 
Published: December 2022

Introductionbluetongue virus (BTV) infection triggers dramatic and complex changes in the host's transcriptional profile to favor its own survival and reproduction. However, there is no whole-transcriptome study of susceptible animal cells with BTV infection, which impedes the in-depth and systematical understanding of the comprehensive characterization of BTV-host interactome, as well as BTV infection and pathogenic mechanisms.Methodsto systematically understand these changes, we performed whole-transcriptome sequencing in BTV serotype 1 (BTV-1)-infected and mock-infected sheep embryonic testicular cells, and subsequently conducted bioinformatics differential analyses.Resultsthere were 1504 differentially expressed mRNAs, 78 differentially expressed microRNAs, 872 differentially expressed long non-coding RNAs, and 59 differentially expressed circular RNAs identified in total. Annotation from the Gene Ontology, enrichment from the Kyoto Encyclopedia of Genes and Genomes, and construction of competing endogenous RNA networks revealed differentially expressed RNAs primarily related to virus-sensing and signaling transduction pathways, antiviral and immune responses, inflammation, and development and metabolism related pathways. Furthermore, a protein-protein interaction network analysis found that BTV may contribute to abnormal spermatogenesis by reducing steroid biosynthesis. Finally, real-time quantitative PCR and western blotting results showed that the expression trends of differentially expressed RNAs were consistent with the whole-transcriptome sequencing data.Discussionthis study provides more insights of comprehensive characterization of BTV-host interactome, and BTV infection and pathogenic mechanisms.
SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain
Authors: Chen Yichen , Zhu Jue , Chen Liang , Shen Yuanyuan , Zhang Jing , Wang Qiming 
Published: December 2022

BackgroundAdenomyosis is an estrogen-dependent gynecological disease. The pathogenesis of chronic pain, the main clinical symptom of adenomyosis, remains undefined. As a combination lymphocyte with both T-cell and natural killer (NK)–cell properties, NK T (NKT) cells play a role in immune defense against numerous diseases and modulate cell differentiation.MethodThis study analyzed the tissue-cell samples from adenomyosis with or without pain by single-cell sequencing.ResultWe found a specific population of secreted frizzled-related protein 4 (SFRP4)+NKT cells and a large amount of undifferentiated multipotent stem cells in the adenomyosis pain group. We discovered that a high expression of IGFBP5 in SFRP4+NKT cells could promote the differentiation of multipotent stem cells into neural-like cells via the single-cell trajectory. Through verification by the sample, we found that the degree of the expression of the neuronal marker NEFM was correlated with the duration of pain in adenomyosis patients. The expression of IGFBP5 was positively correlated with the pain scores of adenomyosis patients.ConclusionCollectively, these findings suggest that SFRP4+IGFBP5hi NKT cells were capable of converting part of the stem cells into neurogenic cells and inducing adenomyosis pain.