bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENERAPAN PEMBELAJARAN KOOPERATIF TIPE TWO STAY TWO STRAY UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS VII SMP NEGERI 11 KOTA BENGKULU
Authors: Efrialinda Aisyah , Yensy Nurul Astuty , Rahimah Dewi 
Published: November 2022

Penelitian bertujuan untuk mengetahui cara meningkatkan hasil belajar dengan penerapan pembelajaran kooperatif tipe two stay two stray. Penelitian ini merupakan tindakan penelitian terhadap suatu Kelas dengan tehnik pengumpulan data melalui tes hasil belajar siswa. Subjek dalam penelitian adalah Kelas VII F SMP N 11 Kota Bengkulu tahun ajaran 2017/2018 berjumlah 20 siswa. Hasil belajar peserta didik dapat ditingkatkan dengan cara memberikan motivasi kepada peserta didik agar lebih baik lagi dalam melaksanakan pembelajaran dan menekankan peserta didik agar lebih teliti dalam mengerjakan soal, dan menanyakan kesulitan belajar yang peserta didik alami. Peningkatan hasil belajar dilihat dari nilai rata-rata peserta didik pada siklus I sampai siklus III yaitu 67,01; 75,75; 86,72 dengan persentase ketuntasan belajar yaitu 35%; 70%; 90%.
Hsa_circ_0074158 regulates the endothelial barrier function in sepsis and its potential value as a biomarker
Authors: Liao Haiyan , Chai Yan , Sun Yuming , Guo Zhe , Wang Xuesong , Wang Ziyi , Wang Ziwen , Wang Zhong 
Published: November 2022

Background: Sepsis is one of the main causes of death in critically ill patients with high morbidity and mortality. Circular RNAs (CircRNAs) are aberrantly expressed, and play significant regulatory roles in many diseases. However, the expression profiles and functions of circRNAs in sepsis have not yet been fully clarified.Methods: Our present study performed an RNA sequencing (RNA-seq) analysis to assess the expression profiles of circRNAs in vitro. We applied the quantitative real-time polymerase chain reaction (RT-qPCR) to verify the RNA-seq results. The analyses of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, the competitive endogenous RNA (ceRNA) regulatory networks, were performed to explore the potential mechanism in sepsis. And then, significantly up-regulated differentially expressed (DE) circRNA, hsa_circ_0074158, was selected for further study. Hsa_circ_0074158 was silenced to investigate its regulatory function in sepsis, and the barrier function was also examined in vitro. Endothelial cell junctions were valued using Vascular endothelial cadherin (VE-cadherin), which was detected by immunofluorescence staining. We measured endothelial permeability by transendothelial electrical resistance (TEER) and fluorescein isothiocyanate (FITC)-dextran extravasation.Results: In total, 203 significantly DE circRNAs, including 77 up-regulated and 126 down-regulated, were identified. In vitro, the RT-qPCR assay showed that the expression pattern of hsa_circ_0074158, hsa_circ_RSBN1L_11059, hsa_circ_0004188, and hsa_circ_0005564 were consistent with the results from RNA-seq analysis. The expression of hsa_circ_0074158 detected by RT-qPCR in vivo was also consistent with the RNA-seq results. The ceRNA networks, GO enrichment, and the KEGG pathway analyses revealed that circRNAs may be related to the barrier function in sepsis. The immunofluorescence assay showed that the suppression of hsa_circ_0074158 expression significantly enhanced the expression of VE-cadherin, which was suppressed in lipopolysaccharide (LPS)-induced sepsis. Additionally, hsa_circ_0074158 knockdown could partially reverse the LPS-induced TEER reduction and FITC-dextran extravasation elevation in sepsis.Conclusion: In conclusion, we have found DE circRNAs could serve as potential biomarkers and therapeutic targets for sepsis. Hsa_circ_0074158 plays a vital role in sepsis and is related to the disruption of the endothelial barrier.
Case report: Familial glycogen storage disease type IV caused by novel compound heterozygous mutations in a glycogen branching enzyme 1 gene
Authors: Li Yiyang , Tian Chuan , Huang Si , Zhang Weijie , Liutang Qiuyu , Wang Yajun , Ma Guoda , Chen Riling 
Published: November 2022

Glycogen storage disease type IV (GSD IV), caused by a mutation in the glycogen branching enzyme 1 (GBE1) gene, is a rare metabolic disorder with an autosomal recessive inheritance that involves the liver, neuromuscular, and cardiac systems. Here, we reported a case of familial GSD IV induced by novel compound heterozygous mutations in GBE1. The proband (at age 1) and her younger brother (at age 10 months) manifested hepatosplenomegaly, liver dysfunction, and growth retardation at onset, followed by progressive disease deterioration to liver cirrhosis with liver failure. During the disease course, the proband presented rare intractable asymptomatic hypoglycemia. The liver pathology was in line with GSD IV. Both cases carried pathogenic compound heterozygous mutations in GBE1 mutations, i.e., a missense mutation (c.271T&gt;A, p. W91R) in exon 2 and a deletion mutation in partial exons 3–7. Both mutations are first reported. The internationally pioneered split-liver transplantation was performed during progression to end-stage liver disease, and the patients had normal liver function and blood glucose after. This study broadens the mutation spectrum of the GBE1 gene and the phenotypic spectrum of GSD IV.
Safety and immunogenicity of heterologous recombinant protein subunit vaccine (ZF2001) booster against COVID-19 at 3–9-month intervals following two-dose inactivated vaccine (CoronaVac)
Authors: Liao Yuting , Chen Yingping , Chen Bo , Liang Zhenzhen , Hu Xiaosong , Xing Bo , Yang Juan , Zheng Qianhui , Hua Qianhui , Yan Chuanfu , Lv Huakun 
Published: November 2022

BackgroundIn response to SARS-CoV-2 mutations and waning antibody levels after two-dose inactivated vaccines, we assessed whether a third dose of recombinant protein subunit vaccine (ZF2001) boosts immune responses.MethodsAn open-label single-center non-random trial was conducted on people aged 18 years and above at five sites in China. All participants received a two-dose inactivated vaccine (CoronaVac) as their prime doses within 3–9 months of the trial. Primary outcomes were safety and immunogenicity, primarily the geometric mean titers (GMTs) of neutralizing antibodies to live wildtype SARS-CoV-2.ResultsA total of 480 participants (median age, 51; range 21–84 years) previously vaccinated with two-dose CoronaVac received a third booster dose of ZF2001 3–4, 5–6, or 7–9-months later. The overall incidence of adverse reactions within 30 days after vaccination was 5.83% (28/480). No serious adverse reactions were reported after the third dose of ZF2001. GMTs in the 3–4-, 5–6-, and 7–9-month groups before vaccination were 3.96, 4.60, and 3.78, respectively. On Day 14, GMTs increased to 33.06, 47.51, and 44.12, respectively. After the booster, GMTs showed no significant difference among the three prime-boost interval groups (all P&gt;0.05). Additionally, GMTs in older adults were lower than those in younger adults on Day 14 for the three groups (P=0.0005, P&lt;0.0001, and P&lt;0.0001).ConclusionHeterologous boosting with ZF2001 was safe and immunogenic, and prime-boost intervals did not affect the immune response. The immune response was weaker in older than younger adults.
Association of plasma brain-derived neurotrophic factor with Alzheimer’s disease and its influencing factors in Chinese elderly population
Authors: Qian Fuqiang , Liu Jian , Yang Hongyu , Zhu Haohao , Wang Zhiqiang , Wu Yue , Cheng Zaohuo 
Published: November 2022

ObjectiveTo explore the association of plasma brain-derived neurotrophic factor (BDNF) levels with Alzheimer’s disease and its influencing factors.Materials and methodsA total of 1,615 participants were included in the present study. Among all subjects, 660 were cognitive normal controls (CNCs), 571 were mild cognitive impairment (MCI) patients, and 384 were dementia with Alzheimer’s type (DAT) patients. BDNF in blood samples collected from these subjects was analyzed via the Luminex assay. Additionally, DNA extraction and APOE4 genotyping were performed on leukocytes using a blood genotyping DNA extraction kit. All data were processed with SPSS 20.0 software. Analysis of variance (ANOVA) or analysis of covariance (ANCOVA) was used to compare differences among groups on plasma BDNF. Pearson and Spearman correlation analysis examined the correlation between BDNF and cognitive impairment, and linear regression analysis examined the comprehensive effects of diagnosis, gender, age, education, and sample source on BDNF.ResultsBDNF levels in DAT patients were higher than those in CNC and MCI patients (P &lt; 0.01). BDNF levels were significantly correlated with CDR, MMSE, and clinical diagnosis (P &lt; 0.001). Age, education, occupation, and sample source had significant effects on BDNF differences among the CNC, MCI, and DAT groups (P &lt; 0.001). BDNF first decreased and then increased with cognitive impairment in the ApoE4-negative group (P &lt; 0.05).ConclusionPlasma BDNF levels decreased in the MCI stage and increased in the dementia stage and were affected by age, education, occupation, and sample source. Unless the effects of sample heterogeneity and methodological differences can be excluded, plasma BDNF is difficult to become a biomarker for the early screening and diagnosis of AD.
Decreased integration of default-mode network during a working memory task in schizophrenia with severe attention deficits
Authors: Cheng Peng , Liu Zhening , Yang Jun , Sun Fuping , Fan Zebin , Yang Jie 
Published: November 2022

BackgroundWorking memory (WM) and attention deficits are both important features of schizophrenia. WM is closely related to attention, for it acted as an important characteristic in activating and manipulating WM. However, the knowledge of neural mechanisms underlying the relationship between WM and attention deficits in schizophrenia is poorly investigated.MethodsGraph theory was used to examine the network topology at the whole-brain and large-scale network levels among 125 schizophrenia patients with different severity of attention deficits (65 mild attention deficits; 46 moderate attention deficits; and 14 severe attention deficits) and 53 healthy controls (HCs) during an N-back WM task. These analyses were repeated in the same participants during the resting state.ResultsIn the WM task, there were omnibus differences in small-worldness and normalized clustering coefficient at a whole-brain level and normalized characterized path length of the default-mode network (DMN) among all groups. Post hoc analysis further indicated that all patient groups showed increased small-worldness and normalized clustering coefficient of the whole brain compared with HCs, and schizophrenia with severe attention deficits showed increased normalized characterized path length of the DMN compared with schizophrenia with mild attention deficits and HCs. However, these observations were not persisted under the resting state. Further correlation analyses indicated that the increased normalized characterized path length of the DMN was correlated with more severe attentional deficits and poorer accuracy of the WM task.ConclusionOur research demonstrated that, compared with the schizophrenia patients with less attention deficits, disrupted integration of the DMN may more particularly underlie the WM deficits in schizophrenia patients with severe attention deficits.
Effects of repetitive peripheral magnetic stimulation on spasticity evaluated with modified Ashworth scale/Ashworth scale in patients with spastic paralysis: A systematic review and meta-analysis
Authors: Pan Jia-Xin , Diao Ying-Xiu , Peng Hui-Yuan , Wang Xi-Zhen , Liao Lin-Rong , Wang Mao-Yuan , Wen You-Liang , Jia Yan-Bing , Liu Hao 
Published: November 2022

BackgroundSpasticity is a common motor disorder resulting from upper motor neuron lesions. It has a serious influence on an individual's motor function and daily activity. Repetitive peripheral magnetic stimulation (rPMS) is a non-invasive and painless approach developed for therapeutic intervention in clinical rehabilitation. However, the effectiveness of this intervention on spasticity in patients with spastic paralysis remains uncertain.ObjectiveThis study aimed to investigate the effectiveness of rPMS on spasticity, motor function, and activities of daily living in individuals with spastic paralysis.MethodsPubMed, PEDro, Embase, Cochrane Library, and Web of Science were searched for eligible papers with date up to March 31, 2022. Two independent researchers conducted study screening, data extraction, and methodological quality assessment. RCTs that explored the effects of rPMS on spasticity, motor function, and activities of daily living in patients with spastic paralysis were included for review. The Cochrane collaboration tool was used to assess methodological quality. The cumulative effects of available data were processed for a meta-analysis using Reedman software.ResultsEight studies with 297 participants were included. Most of the studies presented low to moderate risk of bias. Compared with the control group, the results showed that rPMS had a significant effect on spasticity (all spasticity outcomes: standardized mean difference [SMD] = −0.55, 95% confidence interval [CI]: −0.94 to −0.16, I2 = 40%, and P = 0.006, Modified Ashworth Scale: mean difference [MD] = −0.48, 95% CI: −0.82 to −0.14, I2 = 0%, and P = 0.006), motor function (Fugl–Meyer Assessment: MD = 4.17, 95% CI: 0.89 to 7.46, I2 = 28%, and P = 0.01), and activities of daily living (Barthel Index: MD = 5.12, 95% CI: 2.58 to 7.67, I2 = 0%, and P &lt; 0.0001). No side effect was reported.ConclusionThe meta-analysis demonstrated that the evidence supported rPMS in improving spasticity especially for passive muscle properties evaluated with Modified Ashworth Scale/Ashworth Scale, as well as motor function and daily activity of living in individuals with spastic paralysis.Study registrationThe reviewed protocol of this study is registered in the international prospective register of systematic reviews (PROSPERO) (CRD42022322395).Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier CRD42022322395.
Non-invasive sampling procedure revealing the molecular events at different abutments of bone-anchored hearing systems–A prospective clinical pilot study
Authors: Johansson Martin L. , Omar Omar , Trobos Margarita , Jonhede Sofia , Peters Hanna , Hultcrantz Malou , Thomsen Peter 
Published: November 2022

PurposeTo investigate the molecular activities in different compartments around the bone-anchored hearing system (BAHS) with either electropolished or machined abutments and to correlate these activities with clinical and microbiological findings.Materials and methodsTwelve patients received machined or electropolished abutments after implant installation of BAHS. Peri-abutment fluid and tissue were collected from baseline to 12 months. Gene expression of cytokines and factors related to tissue healing and inflammation, regeneration and remodelling, as well as bacterial recognition were determined using quantitative-polymerase chain reaction (qPCR). The clinical status was evaluated using the Holgers scoring system, and bacterial colonisation was investigated by culturing.ResultsThe gene expression of inflammatory cytokines (IL-8, IL-1β, and IL-10) and bacteria-related Toll-like receptors (2 and 4) was higher in the peri-abutment fluid than at baseline and in the peri-abutment tissue at 3 and 12 months. Conversely, the expression of genes related to tissue regeneration (Coll1a1 and FOXO1) was higher in the tissue samples than in the peri-abutment fluid at 3 and 12 months. Electropolished abutments triggered higher expression of inflammatory cytokines (IL-8 and IL-1β) (in peri-abutment fluid) and regeneration factor FOXO1 (in peri-abutment tissue) than machined abutments. Several cytokine genes in the peri-abutment fluid correlated positively with the detection of aerobes, anaerobes and Staphylococcus species, as well as with high Holger scores.ConclusionThis study provides unprecedented molecular information on the biological processes of BAHS. Despite being apparently healed, the peri-abutment fluid harbours prolonged inflammatory activity in conjunction with the presence of different bacterial species. An electropolished abutment surface appears to be associated with stronger proinflammatory activity than that with a machined surface. The analysis of the peri-abutment fluid deserves further verification as a non-invasive sampling and diagnostic procedure of BAHS.
Deep learning for the diagnosis of suspicious thyroid nodules based on multimodal ultrasound images
Authors: Tao Yi , Yu Yanyan , Wu Tong , Xu Xiangli , Dai Quan , Kong Hanqing , Zhang Lei , Yu Weidong , Leng Xiaoping , Qiu Weibao , Tian Jiawei 
Published: November 2022

ObjectivesThis study aimed to differentially diagnose thyroid nodules (TNs) of Thyroid Imaging Reporting and Data System (TI-RADS) 3–5 categories using a deep learning (DL) model based on multimodal ultrasound (US) images and explore its auxiliary role for radiologists with varying degrees of experience.MethodsPreoperative multimodal US images of 1,138 TNs of TI-RADS 3–5 categories were randomly divided into a training set (n = 728), a validation set (n = 182), and a test set (n = 228) in a 4:1:1.25 ratio. Grayscale US (GSU), color Doppler flow imaging (CDFI), strain elastography (SE), and region of interest mask (Mask) images were acquired in both transverse and longitudinal sections, all of which were confirmed by pathology. In this study, fivefold cross-validation was used to evaluate the performance of the proposed DL model. The diagnostic performance of the mature DL model and radiologists in the test set was compared, and whether DL could assist radiologists in improving diagnostic performance was verified. Specificity, sensitivity, accuracy, positive predictive value, negative predictive value, and area under the receiver operating characteristics curves (AUC) were obtained.ResultsThe AUCs of DL in the differentiation of TNs were 0.858 based on (GSU + SE), 0.909 based on (GSU + CDFI), 0.906 based on (GSU + CDFI + SE), and 0.881 based (GSU + Mask), which were superior to that of 0.825-based single GSU (p = 0.014, p&lt; 0.001, p&lt; 0.001, and p = 0.002, respectively). The highest AUC of 0.928 was achieved by DL based on (G + C + E + M)US, the highest specificity of 89.5% was achieved by (G + C + E)US, and the highest accuracy of 86.2% and sensitivity of 86.9% were achieved by DL based on (G + C + M)US. With DL assistance, the AUC of junior radiologists increased from 0.720 to 0.796 (p&lt; 0.001), which was slightly higher than that of senior radiologists without DL assistance (0.796 vs. 0.794, p &gt; 0.05). Senior radiologists with DL assistance exhibited higher accuracy and comparable AUC than that of DL based on GSU (83.4% vs. 78.9%, p = 0.041; 0.822 vs. 0.825, p = 0.512). However, the AUC of DL based on multimodal US images was significantly higher than that based on visual diagnosis by radiologists (p&lt; 0.05).ConclusionThe DL models based on multimodal US images showed exceptional performance in the differential diagnosis of suspicious TNs, effectively increased the diagnostic efficacy of TN evaluations by junior radiologists, and provided an objective assessment for the clinical and surgical management phases that follow.
Effects of physical activity on the severity of illness and mortality in COVID-19 patients: A systematic review and meta-analysis
Authors: Sittichai Nuttawut , Parasin Nichapa , Saokaew Surasak , Kanchanasurakit Sukrit , Kayod Nuttawan , Praikaew Ketnapa , Phisalprapa Pochamana , Prasannarong Mujalin 
Published: November 2022

Purpose: This systematic review and meta-analysis investigated the association between Physical activity (PA) before Coronavirus Disease 2019 (COVID-19) infection and the severity of illness and mortality in COVID-19 patients.Methods: A comprehensive search was undertaken to identify retrospective and nonrandomized controlled trial studies comparing the severity and mortality of COVID-19 infection among COVID-19 patients who had previously reported their participation in PA with those who had not. The databases searched were PubMed, Cochrane Library, Scopus, Science Direct, EMBASE, OPENGREY.EU, and ClinicalTrials.gov. The risk of bias was assessed using the Newcastle-Ottawa Scale. A random-effects model was used for determining pairwise meta-analyses. The protocol was registered with PROSPERO (CRD42021262548).Results: Eighteen studies met the inclusion criteria (5 cross-sectional, 12 cohort, and 1 case-control studies). All 1 618 680 subjects were adults. PA significantly decreased the risk of death in COVID-19 patients (odds ratio [OR] 0.34; 95% confidence interval [CI], 0.19–0.62; p &lt; 0.001) and the risk of severe outcomes (OR 0.60; 95% CI, 0.48–0.76; p &lt; 0.001). Subgroup analysis showed that PA for ≥150 min/wk at a moderate intensity or ≥75 min/wk at a vigorous intensity reduced the risks of severity and mortality. Vigorous PA reduced mortality risk, whereas moderate to vigorous PA reduced the risks of severity and mortality.Conclusion: PA before infection might reduce severity and mortality in COVID-19 patients, especially PA ≥ 150 min/wk of moderate activity or ≥75 min/wk of vigorous activity. However, careful interpretations should be considered due to the difference in PA patterns and severity definitions among included studies. This finding implies that engaging in regular PA, even in different patterns, has beneficial effects on the severity and mortality of COVID-19 patients.
Moderated-mediation analysis of multimorbidity and health-related quality of life among the Chinese elderly: The role of functional status and cognitive function
Authors: Li Tongxing , Hu Wei , Zhou Liang , Peng Liuming , Cao Lei , Feng Zhaolong , He Qida , Chu Jiadong , Chen Xuanli , Liu Siyuan , Han Qiang , Sun Na , Shen Yueping 
Published: November 2022

ObjectivesTo investigate the relationship between multimorbidity and health-related quality of life (HRQoL), and explore the effects of functional status and cognitive function on Chinses elderly behind this relationship.MethodsThe Multivariate logistic regression and Tobit regression models were used to determine the influence of multimorbidity on HRQoL. Bootstrap analysis was used to probe the mediating effects of functional status and the moderating role of cognition on multimorbidity and HRQoL.ResultsResults of the 2,887 participants age  ≥ 60  years included in the analysis, 51.69% had chronic diseases. Stroke (β = −0.190; 95% confidence interval [CI], −0.232, −0.149; p &lt; 0.001) and the combination of hypertension and stroke (β = −0.210; 95% CI, −0.259, −0.160; p &lt; 0.001) had the greatest influence on HRQoL. Functional status partially mediated the relationship between the number of non-communicable diseases (No. of NCDs) and HRQoL, while cognitive function had a moderating effect not only in the A-path (No. of NCDs to functional status, β = 0.143; t = 7.18; p &lt; 0.001) and but also in the C-path (No. of NCDs to HRQoL, β = 0.007; t = 6.08; p &lt; 0.001).ConclusionFunctional status partially mediated the relationship between multimorbidity and HRQoL in older adults. And cognitive function, if declined, may strengthen this relationship. These findings suggested that improving cognitive function and functional status in those who developed multimorbidity could be a viable prevention or treatment strategy to improve HRQoL in elderly patients.