bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards a bionic IoT: environmental monitoring using smartphone interrogated plant sensors
Authors: Guo Yunlong , Canning John , Chaczko Zenon 
Published: March 2022

AbstractThe utilisation of plants directly as quantifiable natural sensors is proposed. A case study measuring surface wettability of Aucuba japonica, or Japanese Laurel, plants using a novel smartphone field interrogator is demonstrated. This plant has been naturalised globally from Asia. Top-down contact angle measurements map wettability on-site and characterise a range of properties impacting plant health, such as aging, solar and UV exposure, and pollution. Leaves at an early age or in the shadow of trees are found to be hydrophobic with contact angle θ ~ 99°, while more mature leaves under sunlight are hydrophilic with θ ~ 79°. Direct UVA irradiation at λ = 365 nm is shown to accelerate aging, changing contact angle of one leaf from slightly hydrophobic at θ ~ 91° to be hydrophilic with θ ~ 87 ° after 30 min. Leaves growing beside a road with heavy traffic are observed to be substantially hydrophilic, as low as θ ~ 47°, arising from increased wettability with particulate accumulation on the leaf surface. Away from the road, the contact angle increases as high as θ ~ 96°. The results demonstrate that contact angle measurements using a portable diagnostic IoT edge device can be taken into the field for environmental detection, pollution assessment and more. Using an internet connected smartphone combined with a plant sensor allows multiple measurements at multiple locations together in real-time, potentially enabling tracking of parameter change anywhere where plants are present or introduced. This hybrid integration of widely distributed living organic systems with the internet marks the beginning of a new bionic internet-of-things (b-IoT).
Flexible Sensory-Motor Mapping Rules Manifest in Correlated Variability of Stimulus and Action Codes Across the Brain
Authors: van den Brink R.L. , Hagena K. , Wilming N. , Murphy P.R. , Büchel C. , Donner T.H. 
Published: March 2022

SummaryHumans and non-human primates can flexibly switch between different arbitrary mappings from sensation to action to solve a cognitive task. It has remained unknown how the brain implements such flexible sensory-motor mapping rules. Here, we uncovered a dynamic reconfiguration of task-specific correlated variability between sensory and motor brain regions. Human participants switched between two rules for reporting visual orientation judgments during fMRI recordings. Rule switches were either signaled explicitly or inferred by the participants from ambiguous cues. We used behavioral modeling to reconstruct the time course of their belief about the active rule. In both contexts, the patterns of correlations between ongoing fluctuations in stimulus- and action-selective activity across visual and action-related brain regions tracked participants’ belief about the active rule. The rule-specific correlation patterns broke down around the time of behavioral errors. We conclude that internal beliefs about task state are instantiated in brain-wide, selective patterns of correlated variability.
Comparison of sampling methods for small oxbow wetland fish communities
Authors: Osterhaus Dylan M. , Leberg Samuel S. , Pierce Clay L. , Stewart Timothy W. , McCombs Audrey 
Published: March 2022

AbstractThroughout the world, wetlands have experienced degradation and declines in areal coverage. Fortunately, recognition of the value of wetlands has generated interest in preserving and restoring them. Post-restoration monitoring is necessary to analyze success or failure, thereby informing subsequent management decisions. Restoration of oxbow wetlands has become the focus of targeted restoration efforts to promote recovery of biodiversity and sensitive species, and to enhance ecosystem services. The fish communities of oxbows have been the subject of many monitoring studies. However, an optimal method for monitoring the fish communities of oxbows has not been described, thereby limiting our capacity to effectively manage these ecosystems. We compared four sampling methodologies (backpack electrofishing, fyke netting, minnow trapping, and seining) for fish community data collection with a primary objective of determining an optimal method for sampling fish communities in small oxbow wetlands. Seining and fyke netting were determined to be optimal methods for sampling oxbow fish communities. Backpack electrofishing and minnow trapping produced lower total catch and taxonomic richness values than seining and fyke netting. Although seining and fyke netting produced similar taxonomic diversity and abundance values, qualitative analysis revealed that seining caused greater habitat disturbance and potential stress to fish. Therefore, consideration must be given to how species present (especially sensitive species) within the wetland could be impacted by sampling disturbance when choosing between seining and fyke netting.
S. pombe Rtf2 is Important for Replication Fork Barrier Activity of RTS1 via Splicing of Rtf1
Authors: Budden Alice M. , Eravci Murat , Campillo-Funollet Eduard , Naiman Karel , Carr Antony M. 
Published: March 2022

AbstractArrested replication forks, when restarted by homologous recombination, result in error-prone DNA syntheses and non-allelic homologous recombination. Fission yeast RTS1 is a model fork barrier used to probe mechanisms of recombination-dependent restart. RTS1 barrier activity is entirely dependent on the DNA binding protein Rtf1 and partially dependent on a second protein, Rtf2. Human RTF2 was recently implicated in fork restart, leading us to examine fission yeast Rtf2’s role in more detail. In agreement with previous studies, we observe reduced barrier activity upon rtf2 deletion. However, we identified Rtf2 to be physically associated with mRNA processing and splicing factors and rtf2 deletion to cause increased intron retention. One of the most affected introns resided in the rtf1 transcript. Using an intronless rtf1 we observed no reduction in RFB activity in the absence of Rtf2. Thus, Rtf2 is essential for correct rtf1 splicing to allow optimal RTS1 barrier activity.
The mitochondrial genome segregation system ofT. brucei: Single p197 molecules connect the basal body with the outer membrane
Authors: Aeschlimann Salome , Kalichava Ana , Schimanski Bernd , Stettler Philip , Ochsenreiter Torsten , Schneider André 
Published: March 2022

AbstractThe tripartite attachment complex (TAC) couples the segregation of the single unit mitochondrial DNA of trypanosomes with the basal body of the flagellum. Here we studied the architecture of the exclusion zone filament of the TAC that connects the basal body with the mitochondrial outer membrane. The only known component of the exclusion zone filaments is p197. Using genetical, biochemical and microscopical methods we show that p197 has three domains all of which are essential for mitochondrial DNA inheritance. The C-terminus of p197 interacts with the mature and pro-basal body whereas its N-terminus binds to the peripheral outer membrane protein TAC65. The large central region of p197 has a high α-helical content and likely acts as a flexible spacer. Replacement of endogenous p197 with a functional version containing N- and C-terminal epitope tags together with expansion microscopy demonstrates that p197 alone can bridge the approximately 170 nm gap between the basal body and the periphery of the outer membrane. This demonstrates the power of expansion microscopy which allows to localize distinct regions within the same molecule and suggests that p197 is the TAC subunit most proximal to the basal body.Significance statementSegregation of the replicated single unit mitochondrial genome ofTrypanosoma bruceirequires a large hardwired structure that connects the organellar DNA with the flagellar basal body. The cytosolic part of this structure consists of filaments made of single p197 molecules, a protein larger than 600 kDa. p197 has three domains all of which are essential for its function. The N-terminus of p197 is anchored to the peripheral outer membrane protein TAC65 whereas its C-terminus connects to the base of the basal body. The large central domain forms an α-helix and consists of at least 26 repeats of 175 aa in length. It provides a flexible linker bridging the approximately 170 nm between the outer membrane and the basal body
Rickettsia typhi peptidoglycan mapping with data-dependent tandem mass spectrometry
Authors: Oyler Benjamin L. , Rennoll-Bankert Kristen E. , Verhoeve Victoria I. , Rahman M. Sayeedur , Azad Abdu F. , Gillespie Joseph J. , Goodlett David R. 
Published: March 2022

AbstractRickettsia species are diverse Gram-negative obligate intracellular bacteria often pervasive in numerous invertebrates, as well as fungal, nematode and microeukaryotic hosts. Certain species are etiological agents for well-known arthropod-borne illnesses; e.g., R. rickettsii (Rocky Mountain Spotted Fever), R. prowazekii (Epidemic Typhus), and R. typhi, (Endemic Typhus). Living freely in eukaryotic cytosol presumably exposes rickettsiae to host cell immune receptors, particularly those recognizing bacterial cell envelope glycoconjugates. However, the mechanics of host recognition of rickettsiae remain poorly defined. As rickettsiae synthesize a canonical Gram-negative cell envelope that includes peptidoglycan (PGN) and lipopolysaccharide (LPS), structural insight on these macromolecules is important for deciphering host responses to these pathogens. In this work, PGN from R. typhi was digested and the resultant subunits were analyzed by two different, albeit complementary, sample preparation methods. Both approaches were subsequently subjected to liquid chromatography/mass spectrometry analysis to infer PGN structure. R. typhi PGN was determined to be similar to most other Gram-negative bacteria, with mDAP-type muropeptide subunits. However, additional alanine residues were observed elongating the muropeptide stems, rather than the glycine residues usually observed in Gram-negative bacterial PGN. Despite this deviation, R. typhi contains a murein layer that is predicted to agonize host cellular PGN receptors and be susceptible to PGN-targeting antimicrobials. This same structure is likely synthesized by all Rickettsia species, as bioinformatics and comparative genomics analyses indicate the biosynthesis of PGN is highly conserved. Determining how host cells process this canonical glycoconjugate during infection is crucial for identifying factors behind rickettsial pathogenesis, including immunoavoidance or proinflammatory mechanisms possibly employed by rickettsiae with varying pathogenic potential.
Alterations to the broad-spectrum formin inhibitor SMIFH2 improve potency
Authors: Orman Marina , Landis Maya , Oza Aisha , Nambiar Deepika , Gjeci Joana , Song Kristen , Huang Vivian , Klestzick Amanda , Hachicho Carla , Liu Su Qing , Kamm Judith M. , Bartolini Francesca , Vadakkan Jean J. , Rojas Christian M. , Vizcarra Christina L. 
Published: March 2022

ABSTRACTSMIFH2 is a small molecule inhibitor of the formin family of cytoskeletal regulators that was originally identified in a screen for suppression of actin polymerization induced by the mouse formin Diaphanous 1 (mDia1). Despite widespread use of this compound, it is unknown whether SMIFH2 inhibits all human formins. Additionally, the nature of protein/inhibitor interactions remains elusive. We assayed SMIFH2 against human formins representing six of the seven mammalian classes and found inhibitory activity against all formins tested. We synthesized a panel of SMIFH2 derivatives and found that, while many alterations disrupt SMIFH2 activity, substitution of an electron-donating methoxy group in place of the bromine along with halogenation of the furan ring increases potency by approximately five-fold. Similar to SMIFH2, the active derivatives are also pan-inhibitors for the formins tested. This result suggests that while potency can be improved, the goal of distinguishing between highly conserved FH2 domains may not be achievable using the SMIFH2 scaffold.
Structure design and optimization of ground moving and pole climbing inspection robot
Authors: Ye Changlong , Zang Yunfei , Yu Suyang , Jiang Chunying 
Published: March 2022

Purpose
The purpose of this paper is to demonstrate a multipurpose inspection robot that can both walk on the ground and climb on poles. The structure design, size optimization, kinematics analysis, experiment and arithmetic of the robot are discussed in the paper.

Design/methodology/approach
The robot consists of three adjustable modules and a two-degree-of-freedom parallel mechanism in tandem, and the wheel-finger mechanism of each module can realize wheel-finger opening and closing for fast movement and obstacle crossing. This paper uses geometric analysis and simulation analysis to derive size optimization, and vector coordinate method to derive kinematics. Finally, the experiment is carried out by simulating the working environment of the robot.

Findings
The robot can realize ground walking and ground turning through the robot entity prototype experiment on the built working environment and efficiently realize 0°–90° pole climbing by the assemble design, optimization and machining. In addition, the robot can also smoothly complete the state transition process from 0° ground to 90° pole climbing. Furthermore, the robot shows good environmental self-adaptation and can complete daily inspection work.

Originality/value
The robot can pitch and yaw at a large angle and has six-legged characteristics. It is a multipurpose inspection robot that can walk on the ground and climb on poles. And through structure design, size optimization, kinematics analysis and simulation, the existing robots’ common shortcomings such as poor barrier-crossing ability and poor environmental adaptability are solved.
Discovering self-quantified patterns using multi-time window models
Authors: McCully Luke , Cao Hung , Wachowicz Monica , Champion Stephanie , Williams Patricia A.H. 
Published: March 2022

PurposeA new research domain known as the Quantified Self has recently emerged and is described as gaining self-knowledge through using wearable technology to acquire information on self-monitoring activities and physical health related problems. However, very little is known about the impact of time window models on discovering self-quantified patterns that can yield new self-knowledge insights. This paper aims to discover the self-quantified patterns using multi-time window models.Design/methodology/approachThis paper proposes a multi-time window analytical workflow developed to support the streamingk-means clustering algorithm, based on an online/offline approach that combines both sliding and damped time window models. An intervention experiment with 15 participants is used to gather Fitbit data logs and implement the proposed analytical workflow.FindingsThe clustering results reveal the impact of a time window model has on exploring the evolution of micro-clusters and the labelling of macro-clusters to accurately explain regular and irregular individual physical behaviour.Originality/valueThe preliminary results demonstrate the impact they have on finding meaningful patterns.
Corrosion protection by poly(3-hexylthiophene)/poly(methyl-methacrylate) coating of cracked 410 stainless steel
Authors: Conde-Nicho José , Nicho María Elena , Rodríguez José Alfredo , León-Silva Ulises , Rodríguez-Lelis José María 
Published: March 2022

PurposeThis paper aims to study the corrosion protection of 410 stainless steel (410SS) cracked by fatigue tests. The purpose of this study is to show that using polymeric coatings, it is possible to reduce the corrosion rate in metallic structures in operation.Design/methodology/approachPoly(3-hexylthiophene) (P3HT)/poly(methyl-methacrylate) (PMMA) composite was used as a coating to protect the cracked 410SS in the corrosive environment 0.5 M NaCl at 25°C and 80°C. Physicochemical characterization was carried out by adhesion tests, thermogravimetric analysis, nuclear magnetic resonance and size exclusion chromatography. Surface morphology was studied before and after the electrochemical tests by scanning electron microscopy. Uncoated and coated cracked 410SS were characterized by DC electrochemical techniques: linear polarization resistance and potentiodynamic polarization curves.FindingsP3HT/PMMA coating reduced corrosion rate and crack propagation of 410SS in the corrosive medium NaCl 0.5M. The P3HT/PMMA coating increased the polarization resistance by one order of magnitude and decreased the corrosion current density by one order of magnitude, relative to the values obtained with uncoated cracked 410SS. The coating helped to have a less damaged surface and less crack propagation on the cracked 410SS. The feasibility of increasing the useful life of cracked metal structures in a saline environment was demonstrated through polymeric composite coatings.Originality/valueIn the literature, no works were detected that report the use of organic coatings to protect cracked metals against corrosion. This is the first reported work on the corrosion protection of 410SS in a saline medium using coatings based on P3HT/PMMA.
Product innovation, firm performance and moderating role of technology capabilities
Authors: Agustia Dian , Haryanto Sendy Dwi , Permatasari Yani , Midiantari Putu Nidia 
Published: March 2022

PurposeThis research aims to provide evidence that firms that create product innovations (PROINVs) can improve the performance of firms in Indonesia. And then this research also provides evidence that firms with high technological capabilities can moderate the impact of PROINV on firm performance in Indonesia.Design/methodology/approachBased on the firm that disclosure research and development expenditures, the number of samples in this study is 261 annual reports for the period 2015–2020. Researchers used an estimate of Moderation Regression Analysis (MRA) with STATA 14.0.FindingsThe results showed that PROINV significantly affected firm performance, while technology capabilities (TECHCAP) did not. Then TECHCAP can moderate the impact of PROINV on the performance firm. This research showed that TECHCAP are pure moderation, whereas TECHCAP can weaken the impact of PROINV on firm performance.Research limitations/implicationsThis research can provide empirical results that firms in Indonesia must adapt to meet the needs of society in creating PROINVs and technological innovations to maintain a sustainable national economy. And then, this research provides the understanding that high TECHCAP can create sustainable PROINVs that can affect firm performance.Originality/valueThe novelty in this study researchers added variable moderation of technological capabilities to the model of the effect of PROINV on firm performance. A firm with high technological capabilities will be balanced with good research and development activities to create a long-term and sustainable firm orientation.
Understanding undergraduates' work values as a tool to reduce organizational turnover
Authors: Ramírez Itziar , Fornells Albert , Del Cerro Susana 
Published: March 2022

PurposeThe purpose of this research paper is to provide with a strategic approach to reduce organizational turnover by identifying undergraduates' work values. In order to do so, the research undertook two different studies. The first one focused on identifying undergraduates' work values, while the second one explored the alignment between undergraduates' work values and current human resources retention strategies.Design/methodology/approachThe research paper follows a mixed-method approach combining the Work Values Scale (EVAT) applied to 293 hospitality undergraduates of the bachelor's degree in Tourism and Hospitality Management and 30 semi-structured interviews along with 32 semi-structured interviews carried out to human resources professionals. Data was analysed using the EVAT methodology that classifies work values in (openness to change, conservation, self-enhancement and self-transcendence). Relationships between the four dimensions were tested using Pearson correlations and the student profile was analysed comparing the impact of the professional experience and gender in the dimensions.FindingsThe results conclude that undergraduates' work values placed themselves under the categories of self-transcendence and openness to change. There is a significance level in the correlation between self-transcendence and openness to change and work values are influenced by previous professional experience and gender. Human resources professionals tend to overlook work values focusing their retention strategies in providing with training, feedback and promotions but not including practices to promote work–life balance and flexibility.Practical implicationsPractical recommendations are provided for educators with examples of initiatives developed that can be applied in any educational institution.Originality/valueThe research highlights the importance of work motivation based on values as a tool to generate retention strategies.
Do government activities increase public compliance in the Covid-19 pandemic? Evidence from Vietnam
Authors: Thanh Pham Tien , Tung Le Thanh 
Published: March 2022

Purpose
During the COVID-19 pandemic, it has raised an important question of whether government efforts (GEFs), especially communication work, could prevent the outbreak of this pandemic by enhancing public trust and public compliance with the government. This research answers this question by focusing on the case of Vietnam, a low-resource country that gained some achievements during the first pandemic year 2020. In particular, this study aims to investigate the relationship between GEF, public trust and public compliance during the country’s first nationwide social distancing.

Design/methodology/approach
The structural equation modelling is used to analyse data collected from 467 respondents in Vietnam during the nationwide social distancing in 2020.

Findings
Perceived GEF is positively associated with public compliance with the government directly and indirectly through the mediating role of public trust in government (PTG). The empirical results also reveal the effectiveness of government communication work, one among the government’s great efforts, during the COVID-19 pandemic, in terms of intensity and diversity in communication and information channels.

Practical implications
During a pandemic, the governments must enhance public compliance with public health measures to implement the anti-epidemic missions effectively. Therefore, it is important to raise public perception of GEF and PTG. The study’s findings highlight that governments in developing countries should implement relevant responses and policies for the ongoing COVID-19 pandemic and future public health crises, especially in the context that effective vaccines are unavailable or insufficient.

Originality/value
To the best of the authors’ knowledge, this research is among the first attempts that examine the role of GEF, especially public health communication work, in raising public compliance directly and indirectly through the mediating role of public trust. The study carries some important messages for containing a public health crisis like the COVID-19 pandemic.