bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cryptic diversity in a gastrointestinal acanthocephalan of New World primates from Costa Rica
Authors: Rojas-Sánchez Ernesto , Umaña-Blanco Fabián , Jiménez-Rocha Ana , Vega-Benavides Karen , Medaglia Alejandro , Solano-Barquero Alberto , Rojas Alicia , Jiménez Mauricio 
Published: October 2022

Abstract
Prosthenorchis elegans is a worm of the family Archiacanthocephala that infects non-human primates in the Americas, producing an intestinal pathology that may compromise the life of its hosts. Squirrel monkeys, Saimiri oerstedii citrinellus, were found with P. elegans in Costa Rica. Histopathological analysis revealed a severe pyogranulomatous response composed by macrophages, neutrophils, eosinophils, fibroblasts and lymphocytes. Morphological analyses revealed 36 hooks in the proboscis distributed in six rows and total body, hook and lemnisci length compatible to the original descriptions of P. elegans. In addition, phylogenetic, haplotype network and genetic distance analyses were done on cytochrome oxidase subunit 1, cox1, sequences obtained from the collected specimens. Sequences obtained herein clustered separately with high posterior probabilities in a Bayesian Inference tree and showed 8.43% nucleotide differences when compared to P. elegans from Colombia. This high divergence was confirmed in the TCS network that separated Colombian and Costa Rican sequences by 32 mutational steps and a genetic distance PCA which grouped sequences from both geographical locations by 89.5%, indicating the presence of cryptic divergence in P. elegans. Additional studies from specimens collected from other definitive hosts and geographical locations are required to better understand the biodiversity of this species.
D-LMBmap: A fully automated deep learning pipeline for whole-brain profiling of neural circuitry
Authors: Ren Jing , Li Zhongyu , Shang Zengyi , Liu Jingyi , Zhen Haotian , Zhu Entao , Zhong Shilin , Sturgess Robyn , Zhou Yitian , Hu Xuemeg , Wu Yi , Lin Rui 
Published: October 2022

Abstract
Recent proliferation and integration of tissue-clearing methods and light-sheet fluorescence microscopy has created new opportunities to achieve mesoscale 3D whole-brain connectivity mapping with exceptionally high throughput. With the rapid generation of massive high-quality imaging datasets, downstream analysis is becoming the major technical bottleneck for mesoscale connectomics. Current computational solutions are labour-intensive with limited applications because of the exhaustive manual annotation and heavily customised training. Meanwhile, whole-brain data analysis always requires combining multiple packages and secondary development by users. To address these challenges, we developed D-LMBmap, an end-to-end package providing an integrated workflow containing three modules based on novel deep-learning algorithms for whole-brain connectivity mapping: axon segmentation, brain region segmentation and whole-brain registration. D-LMBmap is the first method that does not require manual annotation for axon segmentation, and achieves quantitative analysis of whole-brain projectome in a single workflow with superior accuracy for multiple cell types in all of the modalities tested.
FusionPDB: a unique knowledgebase of human fusion proteins of consistent annotation across genomic/ transcript/ protein/ interaction levels
Authors: Kumar Himansu , Tang Lin-Ya , Yang Chengyuan , Kim Pora 
Published: October 2022

Abstract
Tumorigenic functions due to the formation of fusion genes were targeted for cancer therapeutics (i.e., kinase inhibitors). However, there are still many fusion proteins awaiting being targeted for therapeutics with multiple different mechanisms. Due to the lack of knowledge of the fusion protein sequence and 3D structure, there were not many studies available. To fill this gap, we developed a new computational pipeline and a resource of human fusion proteins, named FusionPDB available at https://compbio.uth.edu/FusionPDB. FusionPDB provides ~ 42K fusion protein sequences (of 16K in-frame fusion genes), 2300 + 1267 fusion protein 3D structures (of 2300 recurrent in-frame fusion genes and 266 manually curated in-frame fusion genes), and virtual screening results of 1267 fusion proteins. FusionPDB is the only resource providing whole 3D structures of fusion proteins and comprehensive knowledge of human fusion proteins. It will be regularly updated until covering all human fusion proteins in the future.
Response categorization and outcomes in extremely premature infants born at 22-26 weeks gestation that received inhaled nitric oxide for hypoxic respiratory failure
Authors: Boly Timothy , Dagle John , Klein Jonathan , Rios Danielle , McNamara Patrick , Giesinger Regan 
Published: October 2022

AbstractObjective: To evaluate the outcomes of extremely premature infants who received inhaled nitric oxide (iNO) for hypoxic respiratory failure (HRF).Study Design: Retrospective analysis of 107 infants born 22-26 weeks gestation who received iNO for HRF at a single institution. Infants were categorized as positive, negative, or no responders based on change in FiO2or OI. Underlying physiology of HRF was determined.Results: 63% of infants had a positive response; they received iNO earlier and were more likely to have acute pulmonary hypertension. Positive response correlated with decreased incidence of death or grade 3 BPD at 36 weeks postmenstrual age, as compared to a negative response.Conclusions: Extremely premature infants have a positive response rate to iNO comparable to term infants when used for acute pulmonary hypertension in the transitional period. Infants with a negative response to iNO had worse outcomes, necessitating the determination of the underlying physiology of HRF prior to iNO initiation.
Aptamer-based Colorimetric and Lateral Flow Immunoassay Detection of Aflatoxin B1 in corn Samples.
Authors: wang yafang , ren wenjie , He baoshan , jin huali , Wei Min , Suo Zhiguang , Xu Yiwei 
Published: October 2022

Abstract
In this paper, gold nanoparticles were combined with aflatoxin B1-aptamer as a single probe via the specific binding between aflatoxinB1 with aptamer, designing a colorimetric detection method. Meanwhile, an immunochromatographic strip with gold nanoparticles modified by complementary chains of aflatoxin B1 aptamer as the probe was developed for rapid detection of aflatoxin B1. Under the optimal conditions, the linear detection range of the colorimetric method was 0.5–50 ng/mL, the minimum detection limit was 0.5 ng/mL, the detection range of the immunochromatographic strip was 0.5–500 ng/mL, the minimum detection limit was 0.5 ng/ mL, and the semi-quantitative limit of detection (LOD) was 51 ng/mL. Finally, comparing the two methods, it is concluded that immunochromatographic strip detection is more sensitive, accurate and celerity. The recoveries of test samples were from 97.3–120%. All detections could be accomplished within 10 min, indicating that the strip could be a potentially effective tool for rapid on-site detections.
Potential protective effect of Silibinin-loaded nanostructured lipid carriers (NLCs) in lead-induced acute nephrotoxicity
Authors: Makhdoomi Sajjad , Ariafar Saba , Sanemar Khashayar , Mohammadi Mojdeh 
Published: October 2022

Abstract
As a toxic heavy metal, lead (Pb) is well known for impairment of renal function due to oxidative injuries. Given the role of silibinin antioxidant activity, the present study investigated the effectiveness of silibinin-loaded nanostructured lipid carriers (Sili-NLCs) against Pb-induced acute nephrotoxicity in rats. The emulsification-solvent evaporation method applied to prepare Sili-NLCs. Sixty male Wistar rats were divided into ten separate groups. Animals were treated with Pb (50 mg/kg/day, i.p.) and with the same concentration of silibinin and Sili-NLCs (25, 50, and 100 mg/kg/day, p.o.) for six days. After sacrificing rats, kidney tissue samples were collected from all animals to assess the oxidative stress parameters. Also, histopathological examination using Hematoxylin-Eosin (H&E) studied. Our data revealed that Pb-induced oxidative damage which indicated by increasing lipid peroxidation (LPO) and nitric oxide (NO) accompanied by decreasing the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity. Moreover, the histopathological examination correlated well with these findings. However, silibinin and especially Sili-NLCs significantly prevented the induction of oxidative damage. Nevertheless, our data suggested that Sili-NLCs are potentially superior to pure silibinin for attenuating Pb-induced acute nephrotoxicity. So, using novel therapeutic approaches is highly required.
Results from a randomised controlled pilot study of the Better Conversations with Primary Progressive Aphasia (BCPPA) communication partner training program for people with PPA and their communication partners.
Authors: Volkmer Anna , Walton H , Swinburn K , Spector A , Warren J D , Beeke S 
Published: October 2022

Abstract
Background:
There has been a growing focus on functional communication interventions for Primary Progressive Aphasia (PPA). These interventions aim to support individuals to participate in life situations. One such intervention, communication partner training (CPT) aims to change conversation behaviours in both the person with PPA and their communication partner (CP). CPT has a growing evidence base in stroke aphasia, however these programs are not designed to meet the needs of people with progressive communication difficulties. To address this, the authors developed a CPT program entitled Better Conversations with PPA (BCPPA) and undertook a pilot trial to establish for a future full trial; predicted recruitment rates, acceptability, an assessment of treatment fidelity and an appropriate primary outcome measure.
Methodology:
This was a single-blind, randomised controlled pilot study comparing BCPPA to no treatment, delivered across 11 National Health Service Trusts in the UK. A random sample of eight recordings of local collaborators delivering the intervention were analysed to examine fidelity. Participants completed feedback forms reporting on acceptability. Pre- and post-intervention measures targeted conversation behaviours, communication goals and quality of life.
Results:
Eighteen people with PPA and their CPs (9 randomised to BCPPA, 9 randomised to no-treatment) completed the study. Participants in the intervention group rated BCPPA positively. Treatment fidelity was 87.2%. Twenty-nine of 30 intervention goals were achieved or over-achieved and 16 of 30 coded conversation behaviours demonstrated change in the intended direction. The Aphasia Impact Questionnaire was identified as the preferred outcome measure.
Conclusion:
The first randomised controlled UK pilot study of a CPT program for people with PPA and their families demonstrates BCPPA is a promising intervention. The intervention was acceptable, treatment fidelity high and an appropriate measure identified. Results of this study indicate a future RCT of BCPPA is feasible.
Trial registration:
Registered 28/02/2018 ISRCTN10148247
Assessing rural household’s food group-source and dietary diversity pattern in Malaita Province
Authors: Bird Zina , Iese Viliamu , Des Combes Helene Jacot , Alungo Bradley , Wairiu Morgan 
Published: October 2022

Abstract
Access to and availability of various food sources is not an issue in rural communities. However, there is no guarantee that households are not affected by nutritional inadequacy, which is still a problem in most underdeveloped nations. A mixed-methods study was conducted to determine the HDDS through snowballing method for the last 12 months' food-source-group and the 48-hour diet recall. Ninety-eight households in 2 rural communities in Malaita participated in the interview between April and May 2019 and analyzed through SPSS and QDA MINER. The aim is to analyze the household dietary patterns of two communities between Zion 1 and Zion 2. The study also hypothesizes that the dietary pattern of households has not changed. A total of 9 food groups; grain, white roots and tubers, plantains, oils/fats (95.90%), condiments (83.70%), and meat/poultry/sea foods (77.60%) were consumed. Majority of the food consumed are from the gardens followed by other food sources which makes up the average HDDS of 5. A correlation analysis found a statistically significant relationship between HDDS and total meal (0.504*) with no relationships between demographic. When comparing 48-hour food sources and food groups to the last year, there was little to no difference in accessibility and availability. This implies that the majority of households are likely to achieve their nutritional needs within the parameters of their dietary trend. Despite the fact that households have adequate food access, there is a significant need to improve their nutritional needs, even if they consume the average required food groups.
A Novel Anisotropy Template for an Improved Interpretation of Elastic Anisotropy Data
Authors: Firdaus Gama , Prasad Manika , Behura Jyoti 
Published: October 2022

AbstractTight unconventional rocks are characterized by the presence of laminations, preferentially oriented cracks, and an interconnected network of compliant minerals. Such anisotropic features can mechanically deform due to pressure depletion during production, leading to a human-induced change of elastic and fluid transport properties. Rock physics models allow us to better predict and assess stress- and direction-dependent elastic moduli of the rock, useful for horizontal stress estimates. However, elastic anisotropy can be challenging to measure and interpret. We have developed an anisotropy template that can be used to assess stress-dependent changes in elastic moduli and investigate rock textures. We present here the template construction using an effective medium model consisting of stiff and compliant layers and crack inclusions and evaluate the origin of stress-dependent stiffness changes in acoustic data from Berea, Bakken, Three Forks, and Mancos formations.
Uncovering transcriptional regulatory network during regeneration for boosting wheat transformation
Authors: Liu Xuemei , Bie Xiao Min , Lin Xuelei , Li Menglu , Wang Hongzhe , Zhang Xiaoyu , Yang Yiman , Zhang Chunyan , Zhang Xian Sheng , Xiao Jun 
Published: October 2022

Abstract
Genetic transformation is important for gene functional study and crop breeding. Though it is available in many plant species, the transformation efficiency in wheat is generally low, which greatly restricts the genetic manipulation in wheat. Here, we use multi-omic analysis strategy to uncover core transcriptional regulatory network (TRN) driving wheat shoot regeneration and identify key factors that boost the transformation efficiency. RNA-seq, ATAC-seq and CUT&Tag were used to profile the transcriptome and chromatin dynamic during regeneration process from immature embryo of wheat variety Fielder. Sequential expression of gene clusters that mediating cell fate transition during regeneration is induced by auxin signaling, in coordination with changes of chromatin accessibility, H3K27me3 and H3K4me3 status. The TRN driving wheat shoot regeneration was built-up and 446 key transcriptional factors (TFs) occupied the core of network were identified, including functionally tested regeneration factors in other species. We further compared the regeneration process between wheat and Arabidopsis and found that DNA binding with one finger (DOF) TFs show distinct patterns in two species. Furthermore, we found that TaDOF5.6 (TraesCS6A02G274000) and TaDOF3.4 (TraesCS2B02G592600) can significantly improve the transformation efficiency of different wheat varieties. Thus, our data uncovers the molecular regulatory insights for wheat shoot regeneration process and provides potential novel targets for improving transformation efficiency in wheat.
Prevalence of Arterial Stiffness Determined by Cardio-ankle Vascular Index in Myeloproliferative Neoplasms
Authors: Jindaluang Thanakharn , Rattarittamrong Ekarat , Chai-Adisaksopha Chatree , Piriyakhuntorn Pokpong , Norasetthada Lalita , Tantiworawit Adisak , Rattanathammethee Thanawat , Hantrakool Sasinee , Hantrakun Nonthakorn , Punnachet Teerachat , Niprapan Piangrawee , Gunaparn Siriluck , Phrommintikul Arintaya 
Published: October 2022

Abstract
Background: Cardiovascular events are the most serious complications for patients with myeloproliferative neoplasms (MPNs). There was limited data regarding arterial stiffness in these patients.
Methods: This was a cross-sectional study aiming to determine the prevalence of arterial stiffness that was evaluated by cardio-ankle vascular index (CAVI) in patients with polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). We defined arterial stiffness as a mean CAVI of 8.0 or more. We compared the prevalence of arterial stiffness in non-MPN patients with cardiovascular risk by matching age, sex, and Thai CV risk score.
Results: A total of 80 patients were enrolled (PV, n = 50; ET, n = 24; PMF, n = 6) with median age of 63.5 years (IQR 50.9-76.1). The prevalence of arterial stiffness in patients with MPNs was 63.8%, and among patients with ET, PV, and PMF was 70.8%, 60.0%, 66.7%, respectively (p = 0.655). After matching, the prevalence of arterial stiffness was not statistically significant difference when compared to the non-MPN population with cardiovascular risk (65.2% vs 60.9%, P = 0.539).
Conclusion The prevalence of arterial stiffness in patients with MPNs was 63.8% which was comparable to non-MPN patients with cardiovascular risk.
The Italian Version of the Pediatric Quality of Life Inventory™ (Pedsql™) 3.0 Healthcare Satisfaction Hematology/oncology Module: Reliability and Validity in Radiation Oncology
Authors: Marconi Elisa , Bartoli Francesco Beghella , Meldolesi Elisa , Mariani Silvia , Panza Giulia , Nardangeli Alessia , Dinapoli Loredana , Lees Teresa Carmen , Guido Antonella , Mastronuzzi Angela , Ruggiero Antonio , Gambacorta Maria Antonietta , Valentini Vincenzo , Balducci Mario , Chieffo Daniela Pia Rosaria , Chiesa Silvia 
Published: October 2022

Abstract
Background
Health-related quality of life (HRQOL) measurement has become an important health care outcome even in oncological pediatric scenario.
During radiation therapy care path, pediatric patients and their relatives may suffer from emotional and psychosocial distress not only related to cancer diagnosis, but also due to the procedure and the required daily routine. Despite the high prevalence of psychosocial consequences in this setting, instruments that inquire pediatric HRQOL and healthcare satisfaction have rarely been studied in Italy.
Purpose of this study was to investigate reliability and linguistic validation of the PedsQL™ healthcare satisfaction Hematology/Oncology module from its original English version to Italian language.
Methods
Three phases standard procedure of cross-culture adaptation were used to create Italian version of PedsQL™ healthcare satisfaction Hematology/Oncology module. Forward translations and backward translations were performed. Finally, a pilot-testing for understandability of the 'pre-final' version was conducted with parents of children attending our Radiotherapy Center using two methodologies of Cognitive Interviewing (“Thinkaloud Interviews” and “Respondent Debriefing”), in order to obtain the final Italian version of the PedsQL™ healthcare satisfaction Hematology/Oncology module.
Results
Twenty-five parents (2 father, 23 mothers) were recruited during their children’s radiotherapy treatment and the grammatically and conceptually acceptable pre-final version of the PedsQL™ Healthcare Satisfaction Hematology/Oncology Module was administered. The questionnaire was well understood reflecting its linguistic adaptation. Compliance with questionnaire administration was optimal. All subjects stated that the questions were interesting to express their opinion, most of them reported that all the questions of each section were clearly comprehensible and easy to understand, suggesting minimal changes that were double-checked with back translation. Furthermore, six of them spontaneously asked to complete the questionnaire in order to review the assistance received during radiotherapy.
Conclusion
Our Italian version of the PedsQL™ 3.0 Healthcare Satisfaction Hematology/Oncology Module seems to be a valid and functional instrument to indagate Healthcare Satisfaction.
Urban bird community assembly mechanisms and driving factors in university campuses in Nanjing, China
Authors: Zhao Zixi , Borzée Amaël , Chen Sheng , Hui Shi , Zhang Yong 
Published: October 2022

Abstract
Understanding the drivers of community assembly process is of great importance for better conservation outcomes; and the main mechanisms include competitive exclusion, environmental filtering and neutral assembly. While mechanisms of assembly processes for vertebrates living in natural habitats have been well studied, their urban counterparts encountering highly human modified environments are still largely understudied. As a result, there are knowledge gaps for urban planners to better protect urban biodiversity and develop eco-friendly cities. University campuses are important components of cities, harboring the majority of urban biodiversity. In this study, based on monthly bird surveys data covering 12 university campuses located either downtown or in the newly developed areas in Nanjing, China, in 2019, we studied the assembly processes of campus’s birds, and their main drivers, by modeling a set of ecological and landscape determinants. Our results showed that bird diversity in the newly developed areas were significantly higher than those downtown. The phylogeny of bird communities in all universities followed a pattern of aggregation, indicating that environmental filtering played a major role in the community assembly. Specifically, grass, water and building were the main factors affecting campus’s bird functional and phylogenetic diversity, with the area of grass and water habitats having a significant positive correlation with phylogenetic diversity while the building areas was negatively correlated. Our results emphasize that habitat features play a decisive role in determining urban bird community assembly processes. We suggest that increasing landscape diversity and improving the landscape connectivity could be a powerful way to maintain and promote urban bird diversity.
Facial expression recognition based on improved Mobile-former
Authors: Xiang Zhang , Chun-man Yan , Miao-miao Sun 
Published: October 2022

Abstract
FER(Facial expression recognition) is a very challenging task in the field of computer vision due to the influence of real-world factors such as illumination, angle, and skin color. With the in-depth study of FER by researchers, CNN(convolutional neural networks) have been widely used in the field of FER due to their excellent local feature extraction ability. In recent years, VIT(Vision Transformer) has become a popular research method for FER due to its excellent global feature processing ability. However, the CNN lacks attention to global features, and VIT has insufficient processing capability for local features, and both face the dilemma of limited application scenarios due to too large parameters. In view of the above problems, this paper first uses Mobile-former as the basic network, so that the network model can combine local and global features when performing expression recognition. Secondly, the ACmix model is introduced to replace the original stem module, making the network can have enough receptive field when initially extracting the input image. Finally, this paper proposes a more lightweight and efficient mobile sub-module to reduce network model parameters. The final experimental results show that the accuracy of the network model in the RAF-DB and CK + datasets is increased by 3.03% and 3% respectively, while the Params is reduced by 1.05M.