bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mutations equivalent to Drosophila mago nashi mutants imply reduction of Magoh protein incorporation into exon junction complex
Authors: Oshizuki Saya , Matsumoto Eri , Tanaka Satoshi , Kataoka Naoyuki 
Published: April 2022

AbstractPre‐mRNA splicing imprints mRNAs by depositing multi‐protein complexes, termed exon junction complexes (EJCs). The EJC core consists of four proteins, eIF4AIII, MLN51, Y14 and Magoh. Magoh is a human homolog of Drosophila mago nashi protein, which is involved in oskar mRNA localization in Drosophila oocytes. Here we determined the effects of Magoh mutations equivalent to those of Drosophila mago nashi mutant proteins that cause mis‐localization of oskar mRNA. We found that Magoh I90T mutation caused mis‐localization of Magoh protein in the cytoplasm by reducing its binding activity to Y14. On the other hand, G18R mutation did not affect its binding to Y14, but this mutation reduced its association with spliced mRNAs. Our results strongly suggest that Magoh mutations equivalent to Drosophila mago nashi mutants cause improper EJC formation by reducing incorporation of Magoh into EJC.
Characterization of two infection‐induced transcription factors of Magnaporthe oryzae reveals their roles in regulating early infection and effector expression
Authors: Cao Yiyang , Chen Jia , Xie Xuze , Liu Shenghua , Jiang Yue , Pei Mengtian , Wu Qianfei , Qi Pengfei , Du Lili , Peng Baoyi , Lan Jianwu , Wu Fan , Feng Ke , Zhang Yifei , Fang Yu , Liu Muxing , Jaber Mohammed Y. , Wang Zonghua , Olsson Stefan , Lu Guodong , Li Ya 
Published: April 2022

AbstractThe initial stage of rice blast fungus, Magnaporthe oryzae, infection, before 36 h postinoculation, is a critical timespan for deploying pathogen effectors to overcome the host's defences and ultimately cause the disease. However, how this process is regulated at the transcription level remains largely unknown. This study functionally characterized two M. oryzae Early Infection‐induced Transcription Factor genes (MOEITF1 and MOEITF2) and analysed their roles in this process. Target gene deletion and mutant phenotype analysis showed that the mutants Δmoeitf1 and Δmoeitf2 were only defective for infection growth but not for vegetative growth, asexual/sexual sporulation, conidial germination, and appressoria formation. Gene expression analysis of 30 putative effectors revealed that most effector genes were down‐regulated in mutants, implying a potential regulation by the transcription factors. Artificial overexpression of two severely down‐regulated effectors, T1REP and T2REP, in the mutants partially restored the pathogenicity of Δmoeitf1 and Δmoeitf2, respectively, indicating that these are directly regulated. Yeast one‐hybrid assay and electrophoretic mobility shift assay indicated that Moeitf1 specifically bound the T1REP promoter and Moeitf2 specifically bound the T2REP promoter. Both T1REP and T2REP were predicted to be secreted during infection, and the mutants of T2REP were severely reduced in pathogenicity. Our results indicate crucial roles for the fungal‐specific Moeitf1 and Moeitf2 transcription factors in regulating an essential step in M. oryzae early establishment after penetrating rice epidermal cells, highlighting these as possible targets for disease control.
Immune complexes as a tool for strongyloidiasis immunodiagnosis in kidney and liver transplant candidate
Authors: Corral Marcelo A. , Gonçalves Ana Lucia R. , Costa Idessania N. , Abdala Edson , Pierrotti Ligia C. , Chieffi Pedro Paulo , Costa‐Cruz Julia Maria , Gryschek Ronaldo Cesar B. , de Paula Fabiana Martins 
Published: April 2022

AbstractStrongyloidiasis is a chronic and asymptomatic infection in immunocompetent patients. Immunocompromised patients, such as organ transplant candidates, can develop severe forms of this disease, and the best way to prevent progression to these forms is early diagnosis. Serological techniques using specific IgG and immune complexes (IC) detection can help in the diagnosis of these patients. This study aimed to detect specific anti‐Strongyloides IC and IgG antibodies in kidney transplant (KT) and liver transplant (LT) candidates. A total of 100 blood samples was collected from transplant candidates (50 blood samples each from KT and LT candidates). Serum was obtained and analysed using enzyme‐linked immunosorbent assay for IC and IgG detections. The IC levels showed frequencies of 18% and 2% in the KT and LT groups, respectively, whereas anti‐Strongyloides IgG was detected in 34% and 12% of KT and LT candidates, respectively. The correlation between IC and IgG detection is poor in KT candidates, while in LT candidates, there is a significant positive correlation. The detection of IC can be an additional tool for the diagnosis of strongyloidiasis, especially when associated with the detection of specific IgG anti‐Strongyloides antibodies.
The Americanisation of English in Brunei
Authors: Sharbawi Salbrina 
Published: April 2022

AbstractPrevious studies investigating postulations that Brunei English was being shaped by American English did not yield any conclusive findings. In recent years, claims of young English‐speaking Bruneians sounding American have become more persistent, thereby precipitating this research. Data was collected from 38 Bruneian Malays who are in their late teens and mid‐twenties and over 900 tokens representative of American English features were analysed. Outcomes reveal that Brunei English is indeed diverging from its traditional exonormative norm, British English, and is displaying new and incipient pronunciations that are indicative of on‐going sound changes. While Americanisation accounts for some of these changes, it is not the only force at play. The findings of this study provide ample evidence that Brunei English is in active state of dynamic change and is steadily progressing towards the next stage of its evolutionary cycle.
Association between the fatty liver index and the risk of severe complications in COVID-19 patients: a nationwide retrospective cohort study
Authors: Chang Yoonkyung , Jeon Jimin , Song Tae-Jin , Kim Jinkwon 
Published: April 2022

AbstractBackgroundResearch on the association of non-alcoholic fatty liver disease (NAFLD) with prognosis in COVID-19 has been limited. We investigated the association between the fatty liver index (FLI), a non-invasive and simple marker of NAFLD, and the severe complications of COVID-19 patients in South Korea.MethodsWe included 3122 COVID-19-positive patients from the nationwide COVID-19 cohort dataset in South Korea between January and June 2020. The FLI was calculated using triglyceride, body mass index, glutamyl transpeptidase, and waist circumference, which were obtained from the national health screening program data. Severe complications related to COVID-19 were defined as the composite of mechanical ventilation, intensive care unit treatment, high-oxygen flow therapy, and death within 2 months after a COVID-19 infection. We performed a multivariate logistic regression analysis for the development of severe complications in COVID-19 patients.ResultsThe mean ± standard deviation of FLI were 25.01 ± 22.64. Severe complications from COVID-19 occurred in 223 (7.14%) patients, including mechanical ventilation in 82 (2.63%) patients, ICU admission in 126 (4.04%), high-flow oxygen therapy in 75 (2.40%), and death in 94 (3.01%) patients, respectively. The multivariate analysis indicated that the highest tertile (T3) of FLI was positively associated with severe complications from COVID-19 (adjusted odds ratio (OR): 1.77, 95% confidence interval (CI) (1.11–2.82), P = 0.017) compared with the lowest tertile (T1).ConclusionsOur study demonstrated that FLI, which represents NAFLD, was positively associated with an increased risk of severe complications from COVID-19. FLI might be used as a prognostic marker for the severity of COVID-19.
Stiffness in breast masses with posterior acoustic shadowing: significance of ultrasound real time shear wave elastography
Authors: Luo Hui , Li Jian , Shi Yang , Xiao Xiaojun , Wang Yuanyang , Wei Zhanghong , Xu Jinfeng 
Published: April 2022

Abstract
Background
To assess the stiffness of benign breast masses in ultrasound images with posterior acoustic shadowing (PAS) and malignant lesions, and explore the significance of differential diagnosis using ultrasound real time shear wave elastography.

Material and methods
All 117 mammary masses (98 patients) with PAS were assessed by using routine ultrasound examination, and elastic modulus values were obtained with the real time shear wave elastography mode. All breast lesions were confirmed by surgery or biopsy. The significance of differences in ultrasound elastography values between breast benign and malignant masses with posterior acoustic shadowing was assessed, and the ROC curves of elasticity modulus values were analyzed.

Results
Among the 117 masses, 72 were benign and 45 were malignant. The two types of breast masses showed significant differences in size, margin, internal echo, calcification, and blood flow characteristics (P < 0.05), although the difference in orientation was not significant (P > 0.05). Emean, Emax and Esd obtained with real time shear wave elastography showed statistically significant differences between benign masses with posterior acoustic shadowing and breast cancer (P < 0.05), while Emin showed no significant difference between them (P = 0.633). Ultrasound real time shear wave elastography showed higher sensitivity and specificity than conventional ultrasound.

Conclusions
Benign and malignant breast masses with PAS show different ultrasound manifestations. Real time shear wave elastography can facilitate the differential diagnosis and treatment planning for these breast masses.
Integrated-Block: A New Combination Model to Improve Web Page Segmentation
Authors: Ghaemmaghami Saeedeh Sadat Sajjadi , Miller James 
Published: April 2022

Context: Web page segmentation methods have been used for different purposes such as web page classification and content analysis. These methods categorize a web page into different blocks, where each block contains similar components.
Objective: The goal of this paper is to propose a new segmentation approach that semantically segments web pages into integrated blocks and obtains high segmentation accuracy.
Method: In this paper, we propose a new segmentation model that semantically segments web pages into integrated blocks, where (1) it merges web page content into basic-blocks by simulating human perception using Gestalt laws of grouping; and, (2) it utilizes semantic text similarity to identify similar blocks and regroup these similar basic-blocks as integrated blocks.
Results: To verify the accuracy of our approach, we (1) applied it to three datasets, (2) compared it with the five existing state-of-the-art algorithms. The results show that our approach outperforms all the five comparison methods in terms of precision, recall, F-1 score, and ARI.
Conclusion: In this paper, we propose a new segmentation model and apply it to three datasets to (1) generate basic-blocks by simulating human perception to segment a web page, (2) identify semantically related blocks and regroup them as an integrated block, and (3) address limitations found in existing approaches.
IMPROVEMENT OF THE METHOD OF IDENTIFICATION AND QUANTITATIVE DETERMINATION OF SULFORAPHANE
Authors: СОНИН С.А. , ЕЛЕЦКАЯ М.И. , ЗАБОЛОТНИЙ А.В. , КАБРАТАЙ И. 
Published: April 2022

Сульфорафан – биологически активное вещество, содержащееся в растениях семейства капустных, используется в качестве физиологически функционального микроингредиента. Отсутствие в РФ аттестованных методов его идентификации и количественного определения в составе пищевых матриц обусловило актуальность настоящих исследований. Определена калибровочная зависимость, разработана методика пробоподготовки и предложен усовершенствованный метод идентификации и количественного определения сульфорафана в липидсодержащих пищевых матрицах, основанный на использовании ВЭЖХ со спектрофотометрическим детектором. Проведена верификация метода, установлены его метрологические характеристики. Метод апробирован на биологически активных добавках, содержащих сульфорафан. Предел обнаружения сульфорафана составляет 0,001%, абсолютная погрешность не превышает 0,008%. Метод может быть рекомендован для использования в исследовательских и производственных лабораториях, оснащенных необходимым оборудованием.
Sulforaphane is a biologically active substance contained in plants of the cabbage family, used as a physiologically functional micro-ingredient. The absence in the Russian Federation of certified methods of its identification and quantitative determination in the composition of food matrices has determined the relevance of these studies. The calibration dependence was determined, a sample preparation technique was developed, and an improved method for the identification and quantitative determination of sulforaphane in lipid-containing food matrices based on the use of HPLC with a spectrophotometric detector was proposed. Verification of the method was carried out; its metrological characteristics were established. The method has been tested on biologically active additives containing sulforaphane. The detection limit of sulforaphane is 0,001%, the absolute error does not exceed 0,008%. The method can be recommended for use in research and production laboratories equipped with the necessary equipment.
vestir como impulso criativo das mudanças políticas:
Authors: Cucinotta Caterina , Patricio Catarina 
Published: April 2022

No dia 6 de janeiro, o assalto ao Capitólio americano na cidade de Washington deixou muitas perplexidades, não só ao nível político, mas também e, sobretudo, ao nível sociocultural. A espetacularização deste evento materializou-se no corpo re-vestido de um dos assaltantes, o qual tomou a posse da iniciativa como líder indiscutível desta ação reivindicatória. Com este artigo pretende-se reflexionar a maneira como a moda, na sua forte ligação com a mídia, pode influenciar a sociedade e a maneira de vestir das pessoas. A relação corpo/vestuário/política é aprofundada através de certas formas de vestir, que parecem expressar opiniões políticas e performances sociais.
Palavras-chaves: Moda; Política; Performance; Corpo revestido.

Abstract
On January 6th, the assault on the U.S. Capitol in the city of Washington left many perplexed, not only on the political level but also and above all on the sociocultural level. The spectacularization of this event materialized in the re-dressed body of one of the robbers, who took possession of the initiative as the undisputed leader of this vindicatory action. With this article we intend to reflect and discover the way fashion in its strong connection with the media can influence society and the way people dress. The relationship body/ clothing/politics is deepened through certain ways of dressing that seems to express political opinions and social performances.
Keywords: Fashion; Politics; Performance; Clothes.
डोटी जिल्लाको जनसांख्यिक अवस्था {Demographic Condition of Doti District}
Authors: बिष्ट Bishta दान बहादुर Dan Bahadur 
Published: April 2022

प्रस्तुत लेखमा डोटी जिल्लाको जनसांख्यिक अवस्थाको वारेमा विभिन्न जनसाख्यिक सूचकहरूको व्याख्या विश्लेषण गरिएको छ । यस लेखमा डोटी जिल्लाको भौगोलिक अवस्था, जनसंख्याको आकार, वृद्धिदर, संरचना, बनोट, वितरण र जनघनत्व सम्बन्धी तथ्यांकहरू संग्रह गरेर जनसंख्याको विश्लेषण गरिएको छ । संघीय संरचना अनुसार डोटी जिल्लाको स्थानीय निकायहरूका आधारमा जनसंख्याको आकार, संरचना, वितरण र जनघनत्व सम्बन्धी तथ्यांकीय विश्लेषण गरिएको छ । यसका साथै यसलेखमा डोटी जिल्लाको वैवाहिक अवस्था, आश्रित जनसंख्या, अशक्त जनसंख्याको प्रकार, आर्थिक हिसावले सक्रिय जनसंख्या, व्यक्तिगत घटना दर्ता प्रणाली र बसाइसराइको अवस्था सम्बन्धी उपलब्ध तथ्यांकको आधारमा विश्लेषण गरिएको छ । डोटी जिल्लाको जनसंख्याको आकार, वृद्धिदर, संरचना, बनोट, वितरण जनघनत्व सम्बन्धी तथ्यांकीय विश्लेषण गरी समग्र डोटी जिल्लाको जनसंख्याको अवस्थावारे जानकारी गराई जिल्लाको आर्थिक, सामाजिक, राजनीतिक, शैक्षिक र लैङ्गिकता सम्बन्धी विभिन्न योजना तर्जुमा, नीतिनिमार्ण गर्ने कार्यमा आंशिक रुपमा भएपनि सहयोग पु¥याउनु प्रस्तुत लेखको उद्देश्य रहेको छ । यो लेख तथ्यांकको द्वितीय स्रोतमा आधारित रहेको छ । यस लेखमा विभिन्न तालिकाहरू, क्रस तालिका, वृत्तचित्र,स्तम्भचित्र, रेखाचित्रहरू प्रयोग गरी तथ्यांकहरूको व्याख्या विश्लेषण गर्ने प्रयास गरिएको छ ।
{The present article analyzes the interpretation of various demographic indicators regarding the demographic situation of Doti district. This article analyzes the population of Doti district by collecting data related to geographical conditions, population size, growth rate, structure, composition, distribution and population density. According to the federal structure, statistical analysis of population size, structure, distribution and population density has been done on the basis of local bodies of Doti district. In addition, this article analyzes the available data on the marital status of Doti district, dependent population, type of disabled population, economically active population, individual incident registration system and the status of migration. Statistical analysis of population size, growth rate, structure, composition, distribution population density of Doti district and providing information about the overall population condition of Doti district. This article is based on a second source of data. This article attempts to analyze the interpretation of facts using various tables, cross tables, documentaries, columns, diagrams.}
Una lumbalgia atípica con una causa infrecuente: espondilodiscitis por neumococo
Authors: Kramer-Ramos Alberto , García-Méndez María del Mar , García-Ortega Ana Azahara , Rosa-Salazar Vladimir , García-Pérez Bartolomé 
Published: April 2022

Describimos el caso de un varón de 13 años sin ningún antecedente de interés con un cuadro de fiebre intermitente y dolor lumbar de 4 semanas de evolución que fue finalmente diagnosticado de espondilodiscitis neumocócica. Resaltar la importancia de esta comunicación, por los escasos casos descritos en la literatura de esta forma de enfermedad neumocócica invasiva, y también por su afectación en un paciente joven y sin patología crónica de base, ya que la espondilodiscitis neumocócica suele afectar a pacientes con edad superior a 50 años y con comorbilidad importante (inmunosupresión, asplenia, diabetes, consumo de alcohol, etc.).
Doble Cushing
Authors: Aguilera-García María , Martínez-Martín Miguel 
Published: April 2022

Varón de 73 años, exfumador, hipertenso y con artritis reumatoide y enfermedad pulmonar intersticial asociada, en tratamiento crónico con prednisona y metotrexato. Inicia estudio por síndrome constitucional, mal control de tensión arterial, y hallazgo de alcalosis metabólica e hipopotasemia. En pruebas complementarias se objetivan datos compatibles con aumento de actividad mineralocorticoide pero con niveles de aldosterona y renina plasmática suprimidos, e hipercortisolismo con confirmación bioquímica de síndrome de Cushing ectópico. Paralelamente se llega al diagnóstico de carcinoma neuroendocrino de células pequeñas como neoplasia justificante del síndrome paraneoplásico.
Metástasis tardías de carcinoma renal de células claras
Authors: Sánchez-Biosca Anna , Ramos-Lázaro Javier , Niño-Aragón Esther , Blancafort-Planas Jaume 
Published: April 2022

El carcinoma renal de células claras es un tumor capaz de producir metástasis tardías, así como en localizaciones poco habituales, por lo que debe incluirse en el diagnóstico diferencial ante el hallazgo de lesiones sugestivas de metástasis en pacientes con antecedentes de este tipo de neoplasia. Se presenta un caso de recidiva metastásica múltiple de un carcinoma renal de células claras tras varios años de haber realizado tratamiento con nefrectomía.
Paciente EPOC con fenotipo agudizador. Caracterización y tratamiento
Authors: Boixeda-Viu Ramón 
Published: April 2022

Varón de 74 años con diagnóstico de EPOC tipo bronquitis crónica con agudizaciones frecuentes y expectoración mucopurulenta persistente que ingresa por una nueva agudización con esputo hemoptoico leve en el que se descartan infecciones oportunistas. En este momento se realiza una caracterización de los fenotipos según la GesEPOC y se ajusta el tratamiento. La reciente actualización de las guías GesEPOC aconsejan una caracterización de los pacientes con EPOC y riesgo alto, estratificando el tratamiento y su manejo en función de los distintos fenotipos descritos.
Neumonía intersticial no específica (NINE) secundaria al tratamiento con imatinib. A propósito de un caso y revisión de la literatura
Authors: Azofra-Macarrón Carlos , Robaina-Cabrera Dimas Manuel , Anta-Fernández Manuela , González-Pascual Andrea , Miguel-Sánchez Carlos de 
Published: April 2022

Imatinib es un inhibidor potente y selectivo de la proteína tirosina kinasa BCR-ABL con eficacia clínica significativa en el tratamiento de la leucemia mieloide crónica (LMC). La toxicidad pulmonar inducida por imatinib tiene un amplio rango de presentación, siendo la neumonitis intersticial un cuadro descrito excepcionalmente en la literatura. Los casos se presentan habitualmente como neumonía intersticial no específica (NINE), y la evolución es generalmente favorable tras la retirada del fármaco y la instauración de tratamiento esteroideo. Presentamos el caso de un paciente varón de 74 años con neumonitis intersticial inducida por imatinib durante el tratamiento de LMC en fase crónica.
EFFECT OF SPORE ATTACHMENT METHODS AND LEVELS ON INVASION AND PARASITISM OF ROOT-KNOT NEMATODE MELOIDOGYNE JAVANICA
Authors: Shahid Muhammad , Gowen Simon R. , Pembroke Barbara 
Published: April 2022

Spore attachment is the first stage in the life cycle of Pasteuria penetrans. Therefore, the present studies were planned to find out whether an attachment level which can provide better reduction in root-knot disease. There was a significant difference (P0.01) regarding numbers of eggmasses produced by females of Meloidogyne javanica in the treatments. Higher numbers of eggmasses (237) were recorded in the treatment where unencumbered J2s were inoculated after spinning followed by the treatment where J2s were inoculated without spores (225) but this difference was not significant. Both spore attachment techniques revealed their influence on eggmass production as fewer eggmasses were recorded in the treatment where encumbered J2s were inoculated compared to controls. Significantly lesser numbers of eggmasses (123) were recorded where J2s were encumbered by exposing them to a spore suspension in plastic trays than by the centrifuge treatment (173). Root galling differed significantly (P0.01) among treatments showing higher root galling in control treatment (5.2). Lesser root galling was observed where J2s were encumbered in the normal manner by exposing nematodes to spore suspension (3.6) while greater root galling (4.0) was observed in the treatment where J2s were encumbered after centrifugation. Similarly, there was a significant difference (P0.01) among females parasitized by P. penetrans showing a higher level of infectivity (13.8) in the treatment where J2s were encumbered by the normal attachment method as compared to the centrifugation method (11.6). There was no significant difference (P0.05) in fresh root weight of plants among treatments. There was no significant difference (P0.05) in number of eggs/eggmass produced by females of M. javanica between the treatments.