bluuebunny/crossref_metadata_2025_split huggingface dataset:
RsCLE22a regulates taproot growth through an auxin signaling-related pathway in radish (Raphanus sativus L.)
Authors: Dong Junhui , Wang Yan , Xu Liang , Li Bingshuang , Wang Kai , Ying Jiali , He Qing , Liu Liwang 
Published: October 2022

Abstract
CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) peptides are a class of small molecules involved in plant growth and development. Although radish (Raphanus sativus) is an important root vegetable crop worldwide, the functions of CLE peptides in its taproot formation remain elusive. Here, a total of 48 RsCLE genes were identified from the radish genome. RNA in situ hybridization showed that RsCLE22a gene was highly expressed in the vascular cambium. Overexpression of RsCLE22a inhibited root growth by impairing stem cell proliferation in Arabidopsis, and radish plants with exogenous supplementation of RsCLE22 peptide (CLE22p) showed a similar phenotype. The vascular cambial activity was increased in RsCLE22a-silenced plants. Transcriptome analysis revealed that CLE22p altered the expression of several genes involved in meristem development and hormone signal transduction in radish. Immunolocalization results showed that CLE22p increased auxin accumulation in vascular cambium. Yeast one-hybrid and dual-luciferase assays showed that the WUSCHEL-RELATED HOMEOBOX 4 (RsWOX4) binds to RsCLE22a promoter and activates its transcription. The expression level of RsWOX4 was related to vascular cambial activity and was regulated by auxin. Furthermore, a RsCLE22a–RsWOX4 module is proposed to regulate taproot vascular cambium activity through an auxin signaling-related pathway in radish. These findings provide novel insights into the regulation of root growth in a horticultural crop.
Association of Interleukin-6 Polymorphisms with Schizophrenia and Depression: A Case-Control Study
Authors: Lu Danyu , Wang Minli , Yang Tongfei , Wang Jianyou , Lin Baiquan , Liu Guoyan , Liang Qiaoyan 
Published: October 2022

Abstract

Objective
Growing evidence suggests a crossover in genetic susceptibility to schizophrenia and depression. We aimed to investigate the association of the rs1800795 and rs1800796 polymorphisms of the IL-6 gene with schizophrenia and depression in the Han Chinese population, combined with IL-6 serum levels.

Methods
Gene sequencing and enzyme-linked immunosorbent assay were performed on 113 subjects with schizophrenia, 114 subjects with depression, and 110 healthy controls.

Results
Our findings showed that IL-6 concentrations in schizophrenia and depression groups were significantly higher than in the control group. The rs1800796 CC genotype and C allele were significantly associated with depression (P = .012 and P &lt; .05, respectively). The rs1800796 CC and CG genotype was significantly associated with chronic schizophrenia (P = .020 and P = .009, respectively). Regarding the rs1800795 polymorphism, only one case of CG genotype was detected. The remainder were of the GG genotype.

Conclusion
The IL-6 rs1800796 might serve as a protective factor for depression and schizophrenia in the Han Chinese population.
Research Priorities Among Canadian Military Veterans Living With Chronic Pain: A Cross-Sectional Survey
Authors: Qureshi Abdul Rehman , Makanjuola Oluwatoni , Neumark Samuel , Garas Andrew , Wang Li , Busse Jason W 
Published: October 2022

ABSTRACT

Introduction
Chronic pain is a debilitating problem that disproportionately affects military veterans. We completed a qualitative study that identified 20 research priorities of Canadian veterans living with chronic noncancer pain. The aim of this study was to establish the generalizability of these priorities.

Materials and Methods
From January to March 2021, we emailed a 45-item survey to a list of Canadian veterans living with chronic noncancer pain that asked about the relative importance of 20 research priorities.

Results
Overall, 313 of 701 Canadian military veterans living with chronic noncancer pain returned a completed survey (45% response rate). All 20 research priorities listed in the survey were endorsed by ≥75% of respondents, and four received ≥95% endorsement: (1) optimizing chronic pain management after release from the military; (2) establishing the effectiveness of self-care; and (3) identifying and (4) treating mental illness among veterans living with chronic pain. One research priority differed significantly by gender; 50% more females than males rated improving chronic pain care while in the military as important (99% vs. 49%, P &lt; .001).

Conclusions
Our survey established research priorities among Canadian veterans living with chronic noncancer pain. These findings should be considered by granting agencies when formulating calls for proposals and by researchers who wish to undertake research that will address the needs of military veterans living with chronic pain.
Mean opacities of a strongly magnetized high-temperature plasma
Authors: Suleimanov Valery F , Mushtukov Alexander A , Ognev Igor , Doroshenko Victor A , Werner Klaus 
Published: October 2022

ABSTRACT
Geometry and dynamical structure of emission regions in accreting pulsars are shaped by the interplay between gravity, radiation, and strong magnetic field, which significantly affects the opacities of a plasma and radiative pressure under such extreme conditions. Quantitative consideration of magnetic plasma opacities is therefore an essential ingredient of any self-consistent modelling of emission region structure of X-ray pulsars (XRPs). We present results of computations of the Rosseland and Planck mean opacities of a strongly magnetized plasma with a simple chemical composition, namely the solar hydrogen/helium mix. We consider all relevant specific opacities of the magnetized plasma including vacuum polarization effect and contribution of electron–positron pairs where the pair number density is computed in the thermodynamic equilibrium approximation. The magnetic Planck mean opacity determines the radiative cooling of an optically thin strongly magnetized plasma. It is by factor of three smaller than non-magnetic Planck opacity at $k_{\rm B}T \lt 0.1\, E_{\rm cyc}$ and increases by a factor of 102–104 at $k_{\rm B}T \gt 0.3\, E_{\rm cyc}$ due to cyclotron thermal processes. We propose a simple approximate expression which has sufficient accuracy for the magnetic Planck opacity description. We provide the Rosseland opacity in a tabular form computed in the temperature range 1–300 keV, magnetic field range 3 × 1010–1015 G, and a broad range of plasma densities. We demonstrate that the scattering on the electron–positron pairs increases the Rosseland opacity drastically at temperatures &gt; 50 keV in the case of mass densities typical for accretion channel in XRPs.
Genetic architecture underlying IgG-RF production is distinct from that of IgM-RF
Authors: Yaku Ai , Ishikawa Yuki , Iwasaki Takeshi , Hiwa Ryosuke , Matsuo Keitaro , Saji Hiroh , Yurugi Kimiko , Miura Yasuo , Furu Moritoshi , Ito Hiromu , Fujii Takao , Maekawa Taira , Hashimoto Motomu , Ohmura Koichiro , Mimori Tsuneyo , Terao Chikashi 
Published: October 2022

Abstract

Objective
HLA-DRB1 alleles, particularly the shared epitope (SE) alleles, are strongly associated with RA. Different genetic structures underlie the production of the various autoantibodies in RA. While extensive genetic analyses have been conducted to generate a detailed profile of ACPA, a representative autoantibody in RA, the genetic architecture underlying subfractions of RF other than IgM-RF, namely IgG-RF, known to be associated with rheumatoid vasculitis, is not well understood.

Methods
We enrolled a total of 743 RA subjects whose detailed autoantibody (IgG-RF, IgM-RF, and ACPA) data were available. We evaluated co-presence and correlations of the levels of these autoantibodies. We analysed associations between the presence or levels of the autoantibodies and HLA-DRB1 alleles for the 743 RA patients and 2008 healthy controls.

Results
We found both IgG-RF(+) and IgG-RF(–) RA subjects showed comparable associations with SE alleles, which was not observed for the other autoantibodies. Furthermore, there was a clear difference in SE allele associations between IgG-RF(+) and (–) subsets: the association with the IgG-RF(+) subsets was solely driven by HLA-DRB1*04:05, the most frequent SE allele in the Japanese population, while not only HLA-DRB1*04:05 but also HLA-DRB1*04:01, less frequent in the Japanese population but the most frequent SE allele in Europeans, were main drivers of the association in the IgG-RF(–) subset. We confirmed that these associations were irrespective of ACPA presence.

Conclusion
We found a unique genetic architecture for IgG-RF(–) RA, which showed a strong association with a SE allele not frequent in the Japanese population but the most frequent SE allele in Europeans. The findings could shed light on uncovered RA pathology.
The association between autoantibodies and risk for venous thromboembolic events among patients with rheumatoid arthritis
Authors: Westerlind Helga , Kastbom Alf , Rönnelid Johan , Hansson Monika , Alfredsson Lars , Mathsson-Alm Linda , Serre Guy , Cornillet Martin , Holmdahl Rikard , Skriner Karl , Bang Holger , Klareskog Lars , Saevarsdottir Saedis , Lundberg Karin , Grönwall Caroline , Askling Johan 
Published: October 2022

Abstract

Objectives
To assess the association between venous thromboembolic (VTE) events and autoantibodies, following patients from RA diagnosis, measuring occurrence, levels and collective load of different autoantibodies against post-translational protein modifications, in particular recognizing citrullination (e.g. citrullinated fibrinogen) and RF by isotype.

Methods
A cohort of 2814 patients with newly diagnosed RA were followed for incident VTE through register linkages. Sera from RA diagnosis were centrally analysed for antibodies to second generation cyclic citrullinated peptides (anti-CCP2), 20 anti-citrullinated protein antibody (ACPA) fine-specificities, antibodies to additional protein modifications (carbamylation and acetylation) and RF by isotype. Association between baseline serology status and future VTE was analysed using Cox regression adjusted for age, sex and calendar period of RA diagnosis, overall and stratified by anti-CCP2 and RF positivity.

Results
During a median 16 years of follow-up, 213 first-ever VTE events were registered (5.0/1000 person-years). IgG anti-CCP2 (present in 65% of cohort) associated with VTE (hazard ratio [HR] = 1.33, 95% CI: 1.00, 1.78), in a dose-response manner. The risk of VTE increased with number of ACPA fine-specificities. IgM RF, but no other RF isotypes, associated with VTE (HR = 1.38, 95% CI: 1.04, 1.82). The associations were independent from smoking and HLA-DRB1 shared epitope alleles. None of the carbamylated or acetylated antibody reactivities associated with VTE.

Conclusion
Anti-CCP2, load of ACPA fine-specificities and IgM RF at RA diagnosis are associated with an increased risk of future VTE in RA. Antibodies to citrullinated fibrinogen did not differ substantially from other ACPA fine-specificities. Autoreactivity to other post-translational modifications was not associated with VTE risk.
Enhancing Nursing Students’ Cultural Competency
Authors: Massarelli Jennifer 
Published: October 2022

Abstract
This project aimed to explore the cultural competence of third-year undergraduate nursing students. A retrospective, quantitative, quasi-experimental design was used to collect data from 15 undergraduate students who took part in a three-week travel abroad community clinical to Thailand during 2019. Students engaged in reflective journaling. Data were collected before and after travel using the 20-item Cultural Intelligence Scale. Post mean scores were higher than presurveys, indicating that students improved on cultural competency overall. All subcategories showed a positive difference, and the results were overall statistically significant.
Exploring cardiac effects after oxytocin 2.5 IU or carbetocin 100 μg
Authors: Bekkenes Maria Egeland , Fagerland Morten Wang , Solberg Ole Geir , Aaberge Lars , Klingenberg Olav , Norseth Jon , Rosseland Leiv Arne 
Published: October 2022

BACKGROUND
Oxytocin can stimulate release of myocardial biomarkers troponin I and T, prolong QTc and induce ST-depression.

OBJECTIVE
To explore cardiac changes after either intravenous carbetocin or oxytocin.

STUDY DESIGN
Exploratory phase 4 randomised controlled trial.

SETTING
Obstetrics units of Oslo University Hospital, Norway between September 2015 and May 2018.

PARTICIPANTS
Forty healthy, singleton pregnant women aged 18 to 50 years at gestational age at least 36 weeks with a planned caesarean delivery.

INTERVENTIONS
Participants were randomised to receive either oxytocin 2.5 IU or carbetocin 100 μg immediately after delivery.

MAIN OUTCOME MEASURES
The primary endpoint was the assessment of troponin I within 48 h of study drug administration. Troponin I and T, and creatine kinase myocardial band assessments were measured before spinal anaesthesia (baseline), and again at 4, 10 and 24 h after delivery. QTc, ST-depression and relative increase in heart rate were recorded from start of study drug administration to 10 min after delivery. All adverse events were monitored.

RESULTS
Compared with the carbetocin group, higher troponin I levels were observed in the oxytocin group at 4 h and 10 h after delivery. For both treatment groups, an increase from baseline in troponin I and T was most pronounced at 10 h after delivery, and it had begun to decline by 24 h. QTc increased with time after administration of both study drugs, with a mean maximum increase of 10.4 ms observed at 9 min (P
< 0.001). No statistical differences were observed in QTc (P = 0.13) or ST-depression (P = 0.11) between the treatment groups.

CONCLUSIONS
Oxytocin 2.5 IU and carbetocin 100 μg caused a similar increase in QTc. The trial was underpowered with regards to ST-depression and the release of myocardial biomarkers and these warrant further investigation. Data from this trial will inform a larger phase 4 trial to determine potential drug differences in troponin release.

TRIAL REGISTRATION
ClinicalTrials.gov Identifier: NCT02528136
Musculoskeletal Health Climate Is a Prognostic Determinant of Sickness Absence Among Female Eldercare Workers
Authors: Ajstrup Merete , Budtz Cecilie Rud , Nielsen Kent Jacob , Andersen Dorte Raaby , Andersen Johan Hviid , Christiansen David Høyrup 
Published: October 2022

Objectives
The present study investigated the association between musculoskeletal health climate, that is, the shared perceptions among workers concerning musculoskeletal health, and sickness absence.

Methods
Questionnaire data on two domains of musculoskeletal health climate, perceived management priority (PMP) and pain acceptance at work (PAW), were collected at baseline. Data on sickness absence were extracted at 1-year follow-up. Data were analyzed using negative binomial multivariable regression.

Results
The final study population comprised 390 female eldercare workers. Compared with participants with low PMP scores, participants with high PMP scores had lower risk of sickness absence (incidence rate ratio, 0.6; 95% confidence interval, 0.4–0.9). Participants with high PAW scores had higher risk of sickness absence than participants with low PAW scores (incidence rate ratio, 1.4; 95% confidence interval, 0.9–2.2).

Conclusions
The results showed an association between the musculoskeletal health climate and sickness absence.
Comprehensive exploration of the expression and prognostic value of AQPs in clear cell renal cell carcinoma
Authors: Wang Huanrui , Zhang Weiyu , Ding Zehua , Xu Tao , Zhang Xiaopeng , Xu Kexin 
Published: October 2022

Aquaporins (AQPs) are a family of membrane water channels that facilitate the passive transport of water across the plasma membrane of cells in response to osmotic gradients created by the active transport of solutes. Water-selective AQPs are involved in tumor angiogenesis, invasion, metastasis and growth. However, the polytype expression patterns and prognostic values of eleven AQPs in clear cell Renal Cell Cancer (ccRCC) have yet to be filled.
We preliminarily investigated the transcriptional expression, survival data and immune infiltration of AQPs in patients with renal cell cancer via the Oncomine database, Kaplan–Meier Plotter, UALCAN cancer database, and cBioPortal databases.
The ethical approval was waived by the local ethics committee of Peking University People's Hospital for the natural feature of mine into databases.
The mRNA expression of AQP1/2/3/4/5/6/7/11 was significantly decreased in ccRCC patients. Meanwhile, MIP and AQP1/2/4/6/7/8/9/11 are notably related to the clinical stage or pathological grade of ccRCC. Lower levels of AQP1/3/4/5/7/10 expression were related to worse overall survival (OS) in patients diagnosed with ccRCC. The AQP mutation rate was 25% in ccRCC patients, but genetic alterations in AQPs were unlikely to be associated with OS and disease free survival in ccRCC patients. In addition, the expression of AQP1, AQP3, AQP4 and AQP10 was positively correlated with immune cells, and the expression of AQP6, AQP7 and AQP11 was negatively correlated with immune cells. AQP9 had a strong and significantly positive correlation with multiple immune cells.
Abnormal expression of AQPs in ccRCC indicated the prognosis and immunomodulatory state of ccRCC. Further study needs to be performed to explore AQPs as new biomarkers for ccRCC.
The angiotensin receptor and neprilysin inhibitor, LCZ696, in heart failure: A meta-analysis of randomized controlled trials
Authors: Chen Yan , He Qian , Mo Dun-Chang , Chen Long , Lu Jia-Lu , Li Rui-Xing , Huang Jie 
Published: October 2022

Background:
LCZ696 is a novel neuroendocrine inhibitor that has been widely used in heart failure (HF). However, its advantage over other neuroendocrine inhibitors, such as angiotensin-converting enzyme inhibitors (ACEis) and angiotensin-receptor blockers (ARBs) has not been fully elucidated. This study aimed to provide the latest evidence regarding the efficacy and safety of LCZ696 as compared to other ACEis and ARBs with regards to the treatment of HF.

Methods:
We systematically searched databases, including PubMed, Embase, and the Cochrane Library, for relevant randomized controlled trials (RCTs). The outcome measures included all-cause mortality, rate of hospitalizations for HF, rate of death from cardiovascular causes, change in N-terminal pro-brain natriuretic peptide (NT-proBNP) levels, and decline of renal function.

Results:
Five RCTs involving 19,078 patients were identified. The meta-analysis indicated that LCZ696 was associated with a significant reduction in all-cause mortality (hazard ratio [HR] = 0.84; 95% confidence interval [CI], 0.76–0.93; P = .0005), rate of hospitalizations for HF (HR = 0.80; 95% CI, 0.73–0.87; P < .00001), reduction in NT-proBNP levels (rate ratio = 0.78; 95% CI, 0.70–0.88; P < .0001), and decline in renal function (odds ratio = 0.77; 95% CI, 0.68–0.88; P < .0001) compared with ACEis and ARBs. However, there was no statistical difference in the rate of death from cardiovascular causes (HR = 0.86; 95% CI, 0.72–1.03; P = .09) between LCZ696 and ACEis and ARBs.

Conclusion:
LCZ696 is superior to ACEis and ARBs in the treatment of HF. Hence, it should be more widely used clinically.
Predictor assessment of complete miscarriage after medical treatment for early pregnancy loss in women with previous cesarean section
Authors: Lu Ye , Su Ruide , Chen Ruixin , Wang Wenrong , An Jian 
Published: October 2022

This study aimed to evaluate clinical predictors associated with complete miscarriage after medical treatment for early pregnancy loss (EPL) in women with previous cesarean section.
Patients with retained uterine content after expulsion followed by administration of mifepristone and misoprostol were included if they chose continued medical treatment rather than surgical intervention. Clinical characteristics including maternal age, gravidity, parity, history of previous cesarean section and ultrasound findings regarding average diameter of the gestational sac, uterine position, width, and blood flow signal of the residual uterine content after expulsion of the gestational sac were included in the analysis to determine predictors of complete miscarriage. A recursive partitioning analysis (RPA) was used to divide the patients into probability groups and assess their probability of complete miscarriage.
A total of 89 patients were analyzed. The complete miscarriage rate was 58.43% overall. Multivariable logistic regression analysis showed that the width and blood flow signal of the residual after expulsion were both independent predictors for complete miscarriage (all P < .05). Patients were divided into high-probability (no blood flow signal, width of residual <1 cm), intermediate-probability (no blood flow signal, width of residual ≥1 cm; blood flow signal, width of residual <1 cm), and low-probability (blood flow signal, width of residual ≥ 1 cm) groups by RPA according to these 2 factors. The incidences of complete miscarriage were 88.24%, 67.57%, and 34.29%, respectively, P < .001).
Surgical evacuation may be avoided in patients without ultrasonic blood flow of the uterine residual and width of the residual <1 cm. More active treatment could be recommended for patients with ultrasonic blood flow of the uterine residual and width of the residual ≥ 1 cm. Clinicians and patients should be aware of these differences when proceeding with medical treatment for EPL patients with previous cesarean section.
Role of 68Ga-FAPI PET/CT in Screening Malignancy of Familial Adenomatous Polyposis
Authors: Erol Fenercioğlu Özge , Yarikkaya Enver , Beyhan Ediz , Çermik Tevfik Fikret , Ergül Nurhan 
Published: October 2022

Abstract
A 57-year-old man with familial adenomatous polyposis (FAP) and newly diagnosed colonic adenocarcinoma was referred to 18F-FDG PET/CT for staging and 68Ga-FAPI-04 PET/CT for ongoing trial. 18F-FDG PET/CT showed equal intense 18F-FDG uptake in the tumor and multiple hypermetabolic polypoid lesions in the entire colorectum. 68Ga-FAPI-04 PET/CT showed intense 68Ga-FAPI-04 uptake only at the colonic tumor, without uptake at polypoid lesions.