bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Physical Activity on Postural Stability and Coordination in Children With Posterior Fossa Tumor: Randomized Control Phase III Trial
Authors: Usama Mahmoud , Abdelaziem Faten , Rashed Wafaa M. , Maher Eslam , Beltagy Mohamed El , Zekri Wael 
Published: August 2022

Abstract
Background: Posterior fossa tumor is a type of brain tumor that is located at the borders of both the brain stem and cerebellum. The cerebellum is the brain region in charge of balance and coordination. Pediatric patients diagnosed with posterior fossa tumor have been reported to fall frequently.Purpose: The aim of this study is to investigate the effectiveness of balance and coordination training in these children.Methods: This randomized control clinical trial (ClinicalTrials.gov Identifier: NCT04528316) was carried out between September 2020 and April 2021 at Children’s Cancer Hospital-57357. The inclusion criteria were - patients with posterior fossa tumor in maintenance phase and, age between 5-12 years. The exclusion criteria were - patients who had a genetic disorder or suffer from mental retardation, a chronic lung disease, sever cardiomyopathy, or a neuromuscular disease that doesn’t not related to tumor. The study participants were randomly assigned into three groups: Group I/Control group: they received Pilates core stability exercises program, Group II/Postural stability group: They received the same program plus HUMAC balance program, and Group III/Coordination group: They received the same program plus coordination exercises of BOT-2. The semi-parametric proportional odds model was used to compare follow up scores of the Postural stability group vs Control, and Coordination group vs Control, while adjusting for baseline values. All tests were two-sided, with alpha set to 0.05.​Results: Sixty children including 38 boys and 22 girls were enrolled in this study. In all three groups, postural stability and coordination improved significantly in terms of modified clinical test of sensory integration of balance, center of pressure, limits of stability, bilateral coordination, and upper-limb coordination.Conclusion: The current study supports the value of adding postural stability and coordination training to the physiotherapy plan for children with posterior fossa tumor.Trial registration number and date of registration: ClinicalTrials.gov Identifier: NCT04528316 on August 27, 2020
Compound odontoma of the lower mandible associated with a deep impacted canine: case report and histologic examination
Authors: MORANDI Benedetta , ALBERTI Alice , TASCHIERI Silvio , SAVADORI Paolo , FRANCETTI Luca , CORBELLA Stefano , FERRIERI Giovanni 
Published: August 2022

Abstract
Even if odontomas are one of the most frequent odontogenic tumors of the oral cavity. no clinical guidelines have been developed to help the clinicians decide in which specific situations the excision of the lesion may be considered beneficial. In this case report a 16 years old male presenting with a compound odontoma and a deep impacted canine has been treated in two different stages: enucleation with histologic examination of the lesion and then extraction of the canine. We highlighted the importance of an early diagnosis, firstly to prevent the odontoma from increasing in size that could complicate the surgical excision, secondly to avoid the compromising of the impacted tooth, serious orthodontic disturbance or jaw distortions.
The significance of presepsin in chronic kidney disease with sepsis
Authors: Wang Wenjuan , He Qingjian , Yuan Jing , Zhang Xiao-Dong , Li Jun-Lu , Zheng Fu-Wen , Kang Jian 
Published: August 2022

Abstract
Background: CKD has a high global prevalence with a consistent estimated global CKD prevalence of between 11 to 13% with the majority stage 3. Patients with CKD undergoing hemodialysis have low immunity and are prone to sepsis. Presepsin is a highly specific biomarker for the early diagnosis of sepsis because presepsin is increased in the blood in the early phase of infection and may be a helpful and valuable biomarker in early diagnosis of sepsis. Therefore, presepsin may have application for the early diagnosis of sepsis in patients with CKD.Purpose This prospective study evaluated the diagnostic value of serum presepsin (soluble CD14-ST) levels for sepsis in chronic kidney disease (CKD) and the influence of undergoing hemodialysis on serum presepsin Methods: This study included 291 patients with CKD and 101 patients with normal renal function and sepsis. Presepsin levels were increased in patients with CKD with or without sepsis or hemodialysis compared to controls, and were highest in patients with CKD and sepsis undergoing hemodialysis. Results: Presepsin levels were similar in patients with CKD with sepsis and no hemodialysis and patients with normal renal function and sepsis, but these levels were lower than in patients with CKD without sepsis undergoing hemodialysis. Presepsin, procalcitonin (PCT) and C-reactive protein (CRP) levels had diagnostic value for sepsis; however, presepsin was a better predictor of sepsis than PCT or CRP in patients with CKD not undergoing hemodialysis. Presepsin level had limited diagnostic value for sepsis in patients with CKD undergoing hemodialysis. In CKD complicated with sepsis, presepsin level was significantly correlated with CRP level, Sequential Organ Failure Assessment score, partial pressure of oxygen and body temperature; there was no correlation between presepsin level and these indicators in CKD without sepsis. Conclusion: These findings suggest physicians should incorporate information on biomarkers with medical history, clinical symptoms, physical signs, and other tests related to sepsis for its diagnosis in patients with CKD undergoing hemodialysis. Presepsin was a better predictor of sepsis in patients with CKD not undergoing hemodialysis.
Copper Adorned Magnetic Nanoparticles as a Heterogeneous Catalyst for Sonogashira Coupling Reaction in Aqueous Media
Authors: Sheikh Safoora , Nasseri Mohammad Ali , Allahresani Ali , Varma Rajender S 
Published: August 2022

Abstract
A nanomagnetic hydrophilic heterogeneous copper catalyst, termed γ-Fe2O3@PEG@PAMAM G0-Cu, has been successfully prepared and characterized using FT–IR, XRD, FE-SEM, TEM, EDX, mapping, TGA/DTG, VSM and ICP analyses. The catalyst displayed excellent activity for the palladium-free Sonogashira cross coupling reaction of various aryl iodides and bromides with phenylacetylene derivatives in pure water. The presence of polyethylene glycol coupled with hydrophilic character of the Cu-catalyst adorned on γ-Fe2O3 MNPs provides the ready dispersion of the catalyst particles in water, leading to higher catalytic performance as well as facile catalyst recovery via simple magnetic decantation. The recovered catalyst was reused for at least six successive runs with little reduction in its catalytic activity and any noticeable changes in its structure. The use of water as a green solvent, without requiring any additive or organic solvent, as well as the exploitation of abundant and low-cost copper catalyst instead of expensive Pd catalyst along with the catalyst recovery and scalability, make this method favorable from environmental and economic points of view for the Sonogashira coupling reaction.
Experiment for Improving the Manufacturing Process of Composite Material Made Serpentine Pipe Parts Using Ceramic Lost Foam Mold
Authors: Yang Shang-Kuo , Chen Lei-Hu , Chu Wen-Lin 
Published: August 2022

Abstract
Nowadays, composite materials are widely used in high-strength and low-weight parts, such as airborne structures, military equipment, sports devices, etc. Since most of the serpentine pipe parts have the requirement of one-piece forming and high strength, the material of serpentine pipe parts is still mainly metal. However, the parts cannot easily separate from the metal molds after autoclave curing is always a difficult problem in the manufacturing process, which results in failed products. The autoclave process is often long and expensive. Therefore, improving its manufacturing yield rate is very important. To solve this problem, this study employs a ceramic lost foam mold to replace the metal mold used in the autoclave process. By adjusting parameters of autoclave in different experiments and experienced several failed experiments, workable manufacturing processes and parameters are finally obtained such that the composite material made serpentine pipe parts are successfully produced. This paper detailed the causes of failed products and corresponding solutions. Moreover, the current study executed process reproducibility experiments to verify the reproducibility of the proposed method. All experiments have been performed in a real workshop.
Targeting ZDHHC9 enhances anti-PD-L1 immunotherapy efficacy by reprogramming the tumour microenvironment
Authors: Lin Zhiqing , Huang Keke , Guo Hui , Jia Manli , Liu Xin , Sun Qiuqin , Chen Xuhao , Wu Jianmin , Yao Qingqing , Zhang Peng , Vakal Sergii , Zou Zhengzhi , Gao Haiyao , Ci Lei , Chen Jiangfan , Guo Wei 
Published: August 2022

Abstract
Background
Immune checkpoint blockade (ICB) therapy targeting the programmed death 1 (PD-1)/programmed death-ligand 1 (PD-L1) axis has achieved considerable success in treating a wide range of cancers. However, most patients are still resistant to ICB and lack of optimal biomarkers to predict response. Thus, uncovering the underlying cellular and molecular determinants of ICB resistance is needed to improve the efficacy of anti-PD-1/L1 therapy. Palmitoylation is mediated by a family of 23 S-acyltransferases, Asp-His‐His‐Cys type (ZDHHC) palmitoyltransferases, that precisely control various cancer-related protein functions, representing promising drug targets for cancer therapy. However, how specific ZDHHC palmitoyltransferases affect the tumour microenvironment (TME) and augment the therapeutic efficacy of ICB remains elusive.
Methods
Five syngeneic subcutaneous tumour models were used to demonstrate the anti-tumour activity of ZDHHC9 deficiency. Multispectral flow cytometry was used to characterize alterations in immune cell subsets. Two poorly immunogenic tumour models were used to evaluate the synergistic effect of ZDHHC9 deficiency and anti-PD-L1 therapy. A polymeric nanoparticle approach was developed for the in vivo delivery of ZDHHC9-siRNA to tumour tissues. The TGCA database was used to assess the expression pattern and immunological characteristics of ZDHHC9 in pan-cancers.
Results
We found that tumor cell-intrinsic “immuno-oncogene” Zinc Finger DHHC-Type Containing 9 (ZDHHC9) was overexpressed in various human cancers and associated with impaired anti-tumor immunity. In five syngeneic tumour models, the knockdown of ZDHHC9 expression in tumour cells was sufficient to suppress tumour progression and improve survival by reprogramming the immunosuppressive (‘cold’) to proinflammatory (‘hot’) TME. Furthermore, ZDHHC9 deficiency in tumour cells sensitized anti-PD-L1 immunotherapy in CD8+ T cells dependent manner. Lastly, we employed the ZDHHC9-siRNA nanoparticle system to efficiently silence ZDHHC9 in tumours whilst potentiating anti-PD-L1 immunotherapy in non-immunogenic tumours.
Conclusion
Tumour-intrinsic S-palmitoylation regulator ZDHHC9 tumour expression is a mechanism of inhibition of host anti-tumour immunity and reprogramming of immunosuppressive tumour microenvironment. Inactivating ZDHHC9 highlights an effective immunotherapeutic strategy and booster for anti-PD-L1 therapy.
Study of the fatigue delamination behaviour of adhesive joints in carbon fibre reinforced epoxy composites, influence of the period of exposure to saline environment
Authors: Argüelles A. , Viña I. , Vigón P. , Lozano M. , Viña J. 
Published: August 2022

Abstract
This work analyses the fatigue delamination and fracture stress behaviour under mode I of adhesive joints made on an epoxy matrix composite material with unidirectional carbon fibre reinforcement and a commercial epoxy-based adhesive. DCB type tests (for mode I) were used with the aim to quantify the influence of the period of exposure to a degradation process in a salt spray chamber, to which the tested samples were subjected, on their fatigue behaviour. For this purpose and after a previous static characterisation of the material in which the critical values of the energy release rate for different exposure periods were determined, the levels of the energy release rate (ERR) to be applied in the fatigue tests and the exposure periods to be considered (no exposure, exposure during one week and twelve weeks) and a ratio of fatigue stress levels of R = Gmin/Gmax=0.1 were defined. From this experimental data, the G-N fatigue initiation curves and the G-da/dN growth curves were obtained. The experimental data obtained, in the fatigue initiation phase of the delamination process, have been treated by means of a probabilistic model based on a Weibull distribution, the application of models of these characteristics has allowed a better interpretation of the experimental results obtained. The most relevant result of the work is that, in general, the fatigue limits obtained for the adhesive joint, under mode I fracture, when subjected to a degradation process in a saline environment, do not translate into a relevant loss of its resistance capacity against this fatigue delamination phenomenon, in its initiation phase. On the other hand, the crack growth rates of the material subjected to different periods of exposure to a saline environment are similar and higher than those obtained for the material without exposure.
Effect of different durations of Shawkea DE-T1 administration on blastocyst obtained rate in women receiving IVF-ET treatment: A secondary analysis of a cohort study
Authors: SHAO Hui , NAKAMOTO Munehiro , YAMAGUCHI Yoji , NOZAKI Toshiaki , DONG Xi , YANG Dongzi , JIAO Shuang , DENG Weifen , KOKEGUCHI Shoji , SHIOTANI Masahide 
Published: August 2022

Abstract
Objective
To explore the appropriate duration of Shawkea DE-T1 use, and to provide a basis for the optimization of the Shawkea DE-T1 administration duration for different women.
Methods
Based on a previous retrospective cohort study, 1,014 patients aged ≥ 30 years who used in vitro fertilization (IVF) for conception at Hanabusa Women’s Clinic, Kobe, Japan, were included in this secondary analysis and were allocated to an Shawkea DE-T1-administration group (n = 712) and a control group (n = 302) based on their use of Shawkea DE-T1. All patients in the two groups received interventions following the guidelines of the Japanese Institution for Standardizing Assisted Reproductive Technology Intervention, and patients in the administration group were provided Shawkea DE-T1 as recommended by the Nutritional Supplement Support Center of Hanabusa Womens Clinic. The blastocyst obtained rate (percentage of patients who produced at least one blastocyst upon in vitro embryo culture relative to all patients in the same group) was compared between the two groups of patients following treatment durations of 1–3 months, 4–6 months, and > 6 months. Analysis was performed on the actual duration of Shawkea DE-T1 administration for all patients who achieved blastocyst in vitro according to their age level (≥ 30 and < 35 years of age; ≥35 and < 40 years; ≥40 and < 43 years; and ≥ 43 years of age).
Results
After a Shawkea DE-T1 administration of 1–3 months or 4–6 months, the blastocyst obtained rates in the administration group were significantly higher than those of the control group (83.27% vs. 55.31% for 1–3 months, P = 1.02×10− 10; 69.44% vs. 44.44% for 4–6 months, P = 4.70×10− 4), while no significant difference was uncovered between the two groups with > 6 months of administration (73.35% vs. 72.46%, P = 0.76). Analysis of the treatment duration of patients at different age levels who produced blastocysts showed that the treatment duration increased commensurate with patient age: i.e., 65.25% of women ≥ 30 and < 35 years of age achieved blastocyst after a Shawkea DE-T1 administration of 1–3 months; while only 19.75% of women ≥ 43 years of age successfully achieved in vitro development of embryos to blastocyst stage with a Shawkea DE-T1 administration of 1–3 months.
Conclusion
Shawkea DE-T1 use for 1–3 months and 3–6 months significantly improved the blastocyst obtained rate in women receiving IVF treatment. Appropriate extension of Shawkea DE-T1 administration duration also achieved a better effect in women of advanced reproductive age.
Improved in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – Confirmed with a wide range of genotypes
Authors: Vollmer Rainer , Espirilla Janeth , Sanchez Juan Carlos , Arroyo Lineo , Acosta Michel , Flores Gonzalo , Rojas Angel , Ellis David , Azevedo Vania 
Published: August 2022

Abstract
In vitro propagation of Plant Genetic Resources is a basic step for routine genebank and biotechnology research activities. Accelerating growth and rooting of in vitro plants contributes to an improvement in process efficiency and plant quality. In the present study the effect of supplemental thiamine and explant size on biometric variables, ion content in plant sap, chlorophyll content in leaves and moisture content in plants were assessed in a replicated trial on a group of seven in vitro sweetpotato accessions and validated in a set of other 45 accessions. It was shown that adding 0.1 mg L -1 of thiamine to modified Murashige and Skoog culture medium significantly increased plant height, root length, and number of nodes of in vitro sweetpotato shoot culture plants. No significant differences were observed for N0 3 - , K + , Na + and Ca ++ - ion content in plant sap, nor in leaf area, chlorophyll, or moisture content between plants grown with or without thiamine. Uninodal stem segments showed on thiamine-free medium a significantly lower root and plant growth, and reduced number of nodes, than plants grown from uni- and binodal segments on thiamine-supplemented medium. A subsequent experiment tested all the parameters above in a non-replicated screen with a set of 45 diverse sweetpotato accessions. With this diverse set of germplasm, the average plant and root length increased by 41 and 51%, respectively on thiamine-supplemented culture medium compared to the control treatment, confirming that supplemental thiamine is generally beneficial to sweetpotato in vitro shoot culture.
Preclinical evaluation of the effect of periodontal regeneration by carbonate apatite in a canine one-wall intrabony defect model
Authors: Takeuchi Shunsuke , Fukuba Shunsuke , Okada Munehiro , Nohara Kohei , Sato Ryo , Yamaki Daichi , Matsuura Takanori , Hoshi Shu , Aoki Kazuhiro , Iwata Takanori 
Published: August 2022

Abstract
Objective: This study aimed to histologically compare periodontal regeneration of one-wall intrabony defects treated with open flap debridement, β-tricalcium phosphate (β-TCP), and CO3Ap in dogs.Materials and Methods: The mandibular third premolars of four beagle dogs were extracted. Twelve weeks after the extraction, a one-wall bone defect of 4 mm × 5 mm (mesio-distal width × depth) was created on the distal side of the mandibular second premolar and mesial side of fourth premolar. Each defect was randomly subjected to open flap debridement only (control group), β-TCP, or CO3Ap treatment. Eight weeks after the surgery, histologic and histometric analyses were performed.Results: No ankylosis, infection, or acute inflammation was observed at any of the experimental sites.　Newly formed bone and cementum were observed in all experimental groups. The ratio of the new bone area was significantly higher in the CO3Ap group than in the control group (P < 0.05). The mineral apposition rate of the alveolar bone crest was higher in the CO3Ap group than in the control and β-TCP groups. The bone contact percentage of the residual granules was significantly higher in the CO3Ap group than in the β-TCP group (P < 0.05).Conclusion: These findings indicate the safety and efficacy of CO3Ap for periodontal regeneration in one-wall intrabony defects in dogs, and CO3Ap is more integrated with bone than β-TCP.Clinical relevance: CO3Ap is compatible with the surrounding bone and provides favorable results for periodontal regeneration in intrabony defects.
Predicting the Activity of Molecules against SARS-CoV-2 using Machine Learning Techniques and Stacked Generalization
Authors: Gousiadou Chrysoula , Sarimveis Haralambos 
Published: August 2022

Abstract
As the pandemic caused by the virus SARS-CoV-2 was rapidly evolving, efforts for the design of new, target-specific molecules active against the viral replication-related enzymes were intensified. On these grounds, the Covid-Moonshot project was launched in March 2020 as a worldwide scientific collaboration aiming to design strong inhibitors against the conserved coronavirus protease Mpro, and subsequently to measure their activity through High Throughput Screening (HTS) assays. As a result, more than 21000 structurally diverse molecules exploring a large chemical space were submitted to the Moonshot project’s website and approximately 10% were evaluated for their inhibitory potency against Mpro. In the present work, profiting from the large chemical space made available through the project and with the advantage of using activity data measured with consistent experimental protocols, we curated informative datasets with which we built and vigorously validated a QSAR stacked regression model as an ensemble of Extreme Gradient Boosting (XGB) and Neural Network (NN) algorithms for estimating the magnitude of the inhibitory potency of molecules against Mpro. The data used for model development and validation contained IC50 values measured with the Fluorescence assay and reported in the project’s on-line database. Our QSAR regression ensemble showed good predictive performance and generalization. Additionally, a set of 78 highly relevant features– including two new engineered - were identified and successfully linked to the inhibitory activity of the modelled compounds. Our findings further suggested that the presence of a halogen-acetamide moiety strongly affected the inhibitory potency of the molecules considered in the present study.
Observation of strong synergy in the interfacial water response of binary ionic and non-ionic surfactant mixtures
Authors: Sengupta Sanghamitra , Gera Rahul , Egan Colin , Morzan Uriel , Versluis Jan , Hassanali Ali , Bakker Huib 
Published: August 2022

Abstract
We have used heterodyne detected vibrational sum-frequency generation spectroscopy (HDVSFG) to probe the interfacial structure of binary mixtures of sodium dodecyl sulfate (SDS) and hexaethylene glycol monododecyl ether (C12E6) surfactants. Our results show that in the presence of C12E6 at CMC (70 µM) the effect of SDS on the orientation of interfacial water molecules is strongly enhanced. Molecular dynamics simulations show that the adsorption free energy of DS− ions to a water surface covered with C12E6 is significantly more attractive by ~ 10 kBT compared to the adsorption energy of DS− to the surface of pure water. The simulations also show that the adsorption of DS− to a water surface covered with C12E6 induces a strong restructuring and enhanced orientation of the near-surface water layers, explaining the HDVSFG results.