bluuebunny/crossref_metadata_2025_split huggingface dataset:
Domestic Violence Protection Orders for Teen Dating Violence: An Analysis of State Laws and Practices
Authors: Lee Jina , Kanaboshi Naoki , Petlakh Ksenia 
Published: August 2022

Purpose: The purpose of this research is to provide an overview of state laws on domestic violence protection orders (DVPO) for teen dating violence. DVPOs are one legal remedy that is available to victims of intimate partner violence to reduce the potential risk of future violence. However, minors are often prohibited from filing a DVPO on their own due to statutory requirements. Method: Using multiple resources and a Westlaw search, we developed a list of state laws pertaining to the statutory requirements for DVPOs for partner-abused minors. We detailed the content of each state’s laws and specific requirements, including self-petitioning, eligible age, dating relationship, and stalking. Additionally, we explored how statutes were interpreted by judicial and non-judicial branches of the governments and nonprofit victim service organizations, and searched other statutory provisions for states where the state statute on the DVPO eligibility for teen dating violence was unclear. Findings: Our findings indicate that state statutes vary in terms of whether minors can be granted DVPOs, whether they can self-petition without a guardian, whether a non-sexual romantic relationship is covered, and whether stalking is covered. Additionally, a variety of courts’ practices and governmental opinions pertaining to protection remedies for child abuse, sexual violence, harassment, or stalking and other statutory provisions other than DVPOs was found. Discussion: This research discusses the importance of more inclusive statutory revisions in each state, and the need to secure greater information accessibility to eligibility requirements, instructions, and procedures for obtaining a DVPO for minors.
Pathological Analysis and Clinical Evolution After Radiological Removal of Retrievable Vena Cava Filters
Authors: Annweiler Thierry , Bertoletti Laurent , Grange Sylvain , Peoc’h Michel , Mismetti Patrick , Barral Fabrice-Guy 
Published: August 2022

Objective Retrievable inferior vena cava filters (IVCF) have been developed because permanent filters have been associated with an increased risk of recurrent deep venous thrombosis. There is no data on the interactions of IVCF with the inferior vena cava (intrafilter thrombi, insertion through the venous wall) even though this may alter the course after retrieval of the IVCF. Methods A review of 85 consecutive patients undergoing retrieval of IVCF placed at a single center was performed from January 1, 2010 and December 31, 2014. Inferior vena cava filter were examined for presence of intrafilter thrombus at time of retrieval. Filter position and presence of intraluminal thrombus were examined. Patient outcomes, including recurrence of deep vein thrombosis (DVT) and death, were captured at 3 month followup. Results Eighty five patients were identified, with intrafilter thrombi found in 69 (81%) patients and venous wall fragments found in 75 (88%) patients. However, their presence was not associated with an increased risk of recurrent venous thromboembolism (VTE) or death during follow up. Conclusions Intrafilter thrombi and venous wall fragments are frequently found in removed IVCF but are not associated with a worse prognosis. They may not modify the therapeutic management of patients.
Laparoscopically Harvested Omental Flap for Immediate Total Breast Reconstruction; Lessons Learnt Through Ten-Year Experience in a Tertiary Oncology Center
Authors: Kahter Ashraf , Ghazy Hosam , Setit Ahmed , Shams Nazem , Gohar Omnia , Abdelwahab Khaled , Eldamshety Osama , Fathi Adel 
Published: August 2022

Background. Most of the publications about Laparoscopically harvested omental flap after skin sparing mastectomy were case reports or case series. Most of them were designed for partial breast reconstruction and were lacking long-term follow-up for cosmetic results, oncologic safety, and detailed methodology. Our study aimed at highlighting the place of this method of reconstruction with long-term oncologic safety and cosmetic outcome. Methods. This study included 95 women who were candidates for skin-sparing mastectomy. Those with body mass index below 25 and above 40, those with omental resection or expected marked adhesions were excluded. All patients were consented to then undergo breast reconstruction using pedicled laparoscopically harvested omental flap. Results. The mean BMI was 34. The mean tumor size was 3.4 cm. The mean total operative time was 129 minutes. Inadequate volume occurred in 12 patients (13%). The mean total drainage output was 750 ml with 8.7% incidence of seroma. Partial breast envelop necrosis was encountered in 5 cases. Partial flap loss occurred in 2 patients. Fat necrosis occurred in 11 cases. Mastectomy and abdominal complications were accepted. Local recurrence was met in one patient and distant metastases occurred in 3 cases. 83.7% of patients expressed excellent and good aesthetic outcomes. Conclusion. When skin sparing mastectomy is chosen with suitable body mass index, laparoscopically harvested omental flap is a very safe and attractive option for total breast reconstruction with a minimal donor site morbidity, satisfactory cosmetic outcome with no compromise of the oncologic safety.
The safety and effectiveness of endovascular treatment for patients with vertebrobasilar artery occlusions: according to the BEST and BASICS criteria
Authors: Sun Wen , Duan Zuowei , Xu Pengfei , Xiao Lulu , Wang Jinjing , Gui Wei , Luo Genpei , Wu Zhongyi , Han Zhongkui , Li Wei , Xu Guoqiang , Liu Fengchang , Yi Jilong , Liu Chaolai , Zhang Yan , Liu Haiyan 
Published: August 2022

Background: Whether endovascular treatment (EVT) is safe and effective for vertebrobasilar artery occlusion (VBAO) is yet incompletely understood. Two RCTs, the endovascular treatment versus standard medical treatment for vertebrobasilar artery occlusion (BEST) trail and the Basilar Artery International Cooperation Study (BASICS), concentrating on this field were recently reported. Objective: We use real-world registry data of VBAO to compare the outcome of EVT inside and outside the inclusion and exclusion criteria of the BEST and BASICS study to testify the feasibility of the selection paradigms of VBAO in these trials. Methods: Consecutive patients with VBAO receiving EVT involving 21 stroke centers were retrospectively included. The safety outcomes [3-month mortality, symptomatic intracranial hemorrhage (sICH), and effectiveness outcomes (the proportion of 3-month functional independence (mRS of 0–2) and favorable outcome (mRS of 0–3)] were compared between VBAO patients who meet or failed to meet the BEST/BASICS selection criteria for EVT. Results: Our study cohort consisted of 577 VBAO patients who underwent EVT. Of them, 446 patients had pc-ASPECTS ≧8. Successful reperfusion (mTICI 2b or 3) was achieved in 85.4% ( n = 493). There were 418 patients fulfilling the BEST criterion for EVT and 194 fulfilling the BASICS criterion. Regression analysis indicated that adherence to BEST or BASICS criterion for EVT was not independently related to most of the safety and effectiveness outcome except that adherence to BEST was significantly associated with the 3-month favorable outcome (ORBEST: 1.742, 95% CI: 1.087–2.790). However, when we put pc-ASPECTS into both criteria with a cut-off value of 8, meeting both BEST criterion plus pc-ASPECTS and BASICS criterion plus pc-ASPECTS was independently related to 3-month functional independence (ORBEST: 1.687, 95% CI: 1.077–2.644; ORBASIC: 1.653, 95% CI: 1.038–2.631) and favorable outcome (ORBEST: 2.280, 95% CI: 1.484–3.502; ORBASIC: 2.153, 95% CI: 1.372–3.378). Conclusion: Our study indicated that, based on real-world data of EVT, adherence to BEST or BASICS criterion for EVT was not independently associated with the safety and effectiveness outcome except that adherence to BEST was significantly related to the 3-month favorable outcome. However, the BEST or BASICS selection criterion and pc-ASPECTS ≧8 might be better paradigms for EVT patient selection.
Malnutrition among under-five children in St. Martin’s Island, Bangladesh: A cross-sectional study on prevalence and associated factors
Authors: Jubayer Ahmed , Islam Md Hafizul , Nayan Md Moniruzzaman 
Published: August 2022

Objectives: Early childhood nutrition is critical for physical and mental development, and any hindrance (malnutrition) at this stage adversely affects adulthood which makes them more susceptible to a variety of communicable and non-communicable diseases. St. Martin’s Island was the focus of this study, which examined the prevalence and risk factors for malnutrition among under-five children. Methods: In this cross-sectional study, 256 children aged under five were assessed for anthropometry, and multiple logistic regression models were fitted to identify potential predictors of malnutrition. For analysis, SPSS and WHO Anthro software were used. Results: The prevalence of stunting, wasting, underweight, and overweight were 34.4%, 17.6%, 18.9%, and 6.9% respectively among children under-five years of age in St. Martin’s Island. An increase in child’s age decreases their risk of being stunted (AOR = 0.97, p = 0.006). Males were more likely than females to be wasted (AOR = 2.03, p = 0.001). Both stunting (AOR = 2.42, p = 0.004) and wasting (AOR = 2.82, p = 0.015) rate were higher among the poorer section as compared to the richest. On the contrary, underweight was low among the poorest section (AOR = 0.37, p = 0.024). Other socio-economic factors, for example, household food insecurity, and parental occupation affect these forms of malnutrition significantly. Conclusion: Prevalence of all forms of malnutrition except underweight was higher as compared to the national perspective in St. Martin’s Island among the under-five children. Community-based approaches by the local government in collaboration with various non-government organizations should be taken to reduce and prevent malnutrition among children.
Study protocol to determine the effects of solvents on lung function among paint industry workers in Mauritius: A cross-sectional study
Authors: Yusuf Khan Yarroo Mohammad , Rathebe Phoka C 
Published: August 2022

Background: The effects of exposure to solvents in paints have been widely studied, but very little information is at hand with only a small number of studies carried out on pulmonary function of paint industry workers. The aim of this study is to determine the respiratory effects of exposure to solvents among workers of the paint industry in Mauritius. Design and methods: A quantitative cross-sectional study will be conducted in three paint factories across Mauritius. 388 participants would be selected using simple random sampling and be subjected to a pre-designed questionnaire for data collection. Lung function test using a spirometer will also be conducted. Data analysis will be performed using Statistical Package for Social Sciences (SPSS v26.0). The exposed workers will be classified by shift duration, that is, individuals with weekly solvent exposure of ≤12 and >12 h respectively. Using multivariable regression analysis, odds ratio will be calculated to analyze the relationship between the dependent variable (hours of exposure to solvents) and independent variables while adjusting for socio-demographic factors. Independent t-test will also be used to identify any difference between the means of lung function tests and the two exposure groups. Based on pre-observation in the three paint industries, it is anticipated that male workers could be found less likely to be exposed to solvents as compared to female workers. Also, workers with a level of education below secondary and lower secondary categories could have a higher likelihood of being exposed ≤12 h/week shift. Those who are aware of occupational hazards and could indicate to have used personal protective equipment (PPE) could be less likely exposed to solvents. Expected Impact of the study for Public Health: This study has been approved by the Ethics Committee of the Faculty of Health Sciences at the University of Johannesburg- ethics clearance number: REC-919-2021. The results of this study will be made available to all stakeholders involved in Health and Safety in the Mauritius. Meetings with professional in the field, especially the concerned paint factories, have been arranged to discuss the impact of solvent exposure and propose remedial actions to protect the workers.
aRNAque: an evolutionary algorithm for inverse pseudoknotted RNA folding inspired by Lévy flights
Authors: Merleau Nono S. C. , Smerlak Matteo 
Published: August 2022

Abstract
Background
We study in this work the inverse folding problem for RNA, which is the discovery of sequences that fold into given target secondary structures.

Results
We implement a Lévy mutation scheme in an updated version of an evolutionary inverse folding algorithm and apply it to the design of RNAs with and without pseudoknots. We find that the Lévy mutation scheme increases the diversity of designed RNA sequences and reduces the average number of evaluations of the evolutionary algorithm. Compared to , CPU time is higher but more successful in finding designed sequences that fold correctly into the target structures.

Conclusion
We propose that a Lévy flight offers a better standard mutation scheme for optimizing RNA design. Our new version of is available on GitHub as a python script and the benchmark results show improved performance on both and the datasets, compared to existing inverse folding tools.
Constructing the boundary between potent and ineffective siRNAs by MG-algorithm with C-features
Authors: Jia Xingang , Han Qiuhong , Lu Zuhong 
Published: August 2022

Abstract
Background
In siRNA based antiviral therapeutics, selection of potent siRNAs is an indispensable step, but these commonly used features are unable to construct the boundary between potent and ineffective siRNAs.

Results
Here, we select potent siRNAs by removing ineffective ones, where these conditions for removals are constructed by C-features of siRNAs, C-features are generated by MG-algorithm, Icc-cluster and the different combinations of some commonly used features, MG-algorithm and Icc-cluster are two different algorithms to search the nearest siRNA neighbors. For the ineffective siRNAs in test data, they are removed from test data by I-iteration, where I-iteration continually updates training data by adding these successively removed siRNAs. Furthermore, the efficacy of siRNAs of test data is predicted by their nearest neighbors of training data.

Conclusions
By siRNAs of Hencken dataset, results show that our algorithm removes almost ineffective siRNAs from test data, gives the clear boundary between potent and ineffective siRNAs, and accurately predicts the efficacy of siRNAs also. We suggest that our algorithm can provide new insights for selecting the potent siRNAs.
Bioinformatic analysis of PD-1 checkpoint blockade response in influenza infection
Authors: Ou Huilin , Chen Keda , Chen Linfang , Wu Hongcheng 
Published: August 2022

Abstract
Background
The programmed cell death 1 (PD-1)/PD-1 ligand 1 (PD-L1) signaling pathway is significantly upregulated in influenza virus infection, which impairs the antiviral response. Blocking this signaling pathway may reduce the damage, lower the virus titer in lung tissue, and alleviate the symptoms of infection to promote recovery. In addition to the enhanced viral immune response, using of immune checkpoint inhibitors in influenza virus infection is controversial, the aim of this study was to identify the key factors and regulatory mechanisms in the PD-1 checkpoint blockade response microenvironment in influenza infection.

Methods
A BALB/c mouse model of influenza A/PR8(H1N1) infection was established then constructed, and whole-transcriptome sequencing including mRNAs, miRNAs (microRNAs), lncRNAs (long noncoding RNAs), and circRNAs (circular RNAs) of mice treated with PD-1 checkpoint blockade by antibody treatment and IgG2a isotype control before infection with A/PR8(H1N1) were performed. Subsequently, the differential expression of transcripts between these two groups was analyzed, followed by functional interaction prediction analysis to investigate gene-regulatory circuits.

Results
In total, 84 differentially expressed dif-mRNAs, 36 dif-miRNAs, 90 dif-lncRNAs and 22 dif-circRNAs were found in PD-1 antagonist treated A/PR8(H1N1) influenza-infected lungs compared with the controls (IgG2a isotype control treated before infection). In spleens between the above two groups, 45 dif-mRNAs, 36 dif-miRNAs, 57 dif-lncRNAs, and 24 dif-circRNAs were identified. Direct function enrichment analysis of dif-mRNAs and dif-miRNAs showed that these genes were mainly involved in myocardial damage related to viral infection, mitogen activated protein kinase (MAPK) signaling pathways, RAP1 (Ras-related protein 1) signaling pathway, and Axon guidance. Finally, 595 interaction pairs were obtained for the lungs and 462 interaction pairs for the spleens were obtained in the competing endogenous RNA (ceRNA) complex network, in which the downregulated mmu-miR-7043-3p and Vps39–204 were enriched significantly in PD-1 checkpoint blockade treated A/PR8(H1N1) infection group.

Conclusions
The present study provided a basis for the identification of potential pathways and hub genes that might be involved in the PD-1 checkpoint blockade response microenvironment in influenza infection.
Upregulation of key genes Eln and Tgfb3 were associated with the severity of cardiac hypertrophy
Authors: Zhang Rui , Xu Xuan , Chen Xi , Hao Chunshu , Ji Zhenjun , Zuo Pengfei , Yang Mingming , Ma Genshan , Li Yongjun 
Published: August 2022

Abstract
Background
Hypertension-induced cardiac hypertrophy is one of the most common pre-conditions that accompanies heart failure. This study aimed to identify the key pathogenic genes in the disease process.

Methods
GSE18224 was re-analyzed and differentially expressed genes (DEGs) were obtained. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were carried out. Networks of transcription factor (TF)-mRNA, microRNA (miRNA)-mRNA and Protein-Protein interaction (PPI) were constructed, and a key module was further screened out from PPI network. GSE36074 dataset and our transverse aortic constriction (TAC) mouse model were used to validate gene expression in the module. Finally, the correlation between the genes and biomarkers of cardiac hypertrophy were evaluated.

Results
Totally, there were 348 DEGs in GSE18224, which were mainly enriched in biological processes including collagen fibril organization, cellular response to transforming growth factor-beta stimulus and were involved in ECM-receptor interaction and Oxytocin signaling pathway. There were 387 miRNAs targeted by 257 DEGs, while 177 TFs targeted 71 DEGs. The PPI network contained 222 nodes and 770 edges, with 18 genes screened out into the module. After validation, 8 genes, which were also significantly upregulated in the GSE36074 dataset, were selected from the 18 DEGs. 2 of the 8 DEGs, including Eln and Tgfb3 were significantly upregulated in our mouse model of myocardial hypertrophy. Finally, the expression of Eln and Tgfb3 were found to be positively correlated with the level of the disease biomarkers.

Conclusions
Upregulated key genes Eln and Tgfb3 were positively correlated with the severity of cardiac hypertrophy, which may provide potential therapeutic targets for the disease.
High-throughput transcriptome sequencing reveals the key stages of cardiovascular development in zebrafish embryos
Authors: Zhou Chune , Zhao Wei , Zhang Shuqiang , Ma Junguo , Sultan Yousef , Li Xiaoyu 
Published: August 2022

Abstract
Background
The cardiovascular developmental process is a tightly regulated network involving multiple genes. The current understanding of the molecular mechanism behind cardiovascular development is insufficient and requires further research.

Results
Transcriptome sequencing of three developmental stages in zebrafish embryos was performed and revealed three key cardiovascular developmental stages. Then, the differentially expressed genes (DEGs) involved in cardiovascular development were screened out. The three developmental stages were 18 (T1), 24 (T2), and 42 h post fertilization (hpf) (T3), and the three stages were confirmed by detecting differences in expression between cardiomyocyte and endothelial marker genes (cmlc2, fli1) using in situ hybridization, which represents the characteristics of cardiovascular development. Thousands of DEGs were identified using transcriptome analysis. Of them, 2605 DEGs were in T1-vs-T2, including 2003 up-regulated and 602 down-regulated genes, 6446 DEGs were in T1-vs-T3, consisting of 4608 up-regulated and 1838 down-regulated genes, and 3275 DEGs were in T2-vs-T3, including 2420 up-regulated and 855 down-regulated genes. There were 644 common DEGs and 167 common five-fold higher differentially expressed genes (HDEGs) identified, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID). Significant differences was observed in the levels of gene expression among different developmental stages in multiple GO terms and KEGG pathways, such as cell migration to the midline involved in heart development, cardiovascular system development, circulatory system process for biological processes of GO terms; and cardiac muscle contraction, adrenergic signaling in cardiomyocytes for KEGG pathways. These results demonstrated that these three stages were important period for the development of the cardiovascular system. Lastly, we used quantitative real-time PCR (qPCR) to validate the reliability of RNA-sequencing by selecting 21 DEGs.

Conclusions
These results demonstrated that these three stages represented the important periods for cardiovascular system development of zebrafish and some candidate genes was obtained and provided a solid foundation for additional functional studies of the DEGs.
A qualitative research study of primary care physicians’ views of telehealth in delivering postnatal care to women
Authors: Poon Zhimin , Tan Ngiap Chuan 
Published: August 2022

Abstract
Background
The postpartum period is a critical time for women to optimise their physical and mental health. Primary care physicians (PCP) often manage postpartum women in the community setting after uneventful births. However, women encounter difficulties accessing care before and after their conventional 6-week physical review. Telehealth-based interventional studies have demonstrated their successful applications in several areas of postpartum care but is not widely adopted. The study aimed to explore the PCPs’ views on their acceptability and perceived barriers of telehealth in delivering postpartum care to women in primary care.

Methods
Twenty-nine PCPs participated in eleven in-depth interviews and four focus group discussions for this qualitative study conducted in Singapore. The purposively sampled PCPs had varied demographic background and medical training. Two investigators independently coded the audited transcripts. Thematic content analysis was performed using the codes to identify issues in the pertaining to the perceived usefulness, ease of use and attitudes towards telehealth in postpartum care as described in the “Telehealth Acceptance Model” framework.

Results
Most PCPs perceived usefulness and ease of use of video consultation in delivering postpartum care. They recognised telehealth service to complement and support the current face-to-face postpartum care amidst the pandemic. However, training, leadership support, organizational infrastructure, healthcare financial policy and personal demographic profile influence their acceptance of a new care model for postnatal mothers.

Conclusion
Addressing the barriers and strengthening the facilitators will enhance PCPs’ acceptance and utilisation of the proposed hybrid (telehealth and in-person) postnatal care model for mothers.