bluuebunny/crossref_metadata_2025_split huggingface dataset:
Demographic, clinical and laboratory findings of severe and non-severe COVID-19 cases: one-year experience at a private set-up of Bangladesh
Authors: Saha Madhurima , Saha Debasish Kumar , Bala Chandra Shekhar , Sutradhar Pankaj Kanti , Hasan Md Asif , Ferdous Chowdhury Nawshin , Muniya Shabnaz , Zabeen Nazia 
Published: May 2022

Background: COVID-19 has emerged in Dhaka city of Bangladesh since March 8, 2020 and rapidly spread throughout the country. Though non-severe cases can be managed in home, severe cases are more likely to be hospitalized and need intensive care unit (ICU) admission. Understanding the common demographic, clinical and laboratory features of severe versus non-severe patients could be quite useful for clinicians and might help to predict disease progression. This study aimed to compare demographic, clinical and laboratory findings among severe and non-severe COVID-19 cases.
Methods: This retrospective study was conducted on confirmed COVID-19 patients who were admitted in a private set-up of Bangladesh, from 1st April 2020 to 31st March 2021. All confirmed COVID-19 cases were categorized into severe (severe/critical) and non-severe (asymptomatic/mild/moderate) group. The demographic, clinical and laboratory parameters were recorded. Patients’outcome was categorized by discharged (recovered), transferred to higher center (referred) and death.
Results: Among 219 COVID-19 patients, 139 (78 males and 61 females) were in non-severe and 80 patients (56 males and 24 females) were in severe group. There was older age and male predominance among severe than nonsevere infection (pÂ0.05). The co-morbidities and smoking history showed non-significant differences between the groups. Among the presenting symptoms, cough and dyspnoea were present 91.3% and 61.3% respectively in severe vs 75.5% and 27.3% respectively in non-severe group. There was significantly lower percentages of lymphocyte (19.8 ± 12.6 %); higher values of ferritin (ng/mL) and D-dimer (mg/l) [1384.50 (103 - 2898) and 0.74 (0.03 - 883) respectively] among patients with severe COVID-19, pÂ0.05. On chest x-ray, pneumonitis was present in 69 (95.8%) patient in severe group, whereas 90 (78.3%) in non-severe group and the difference was statistically significant (pÂ0.05). High-resolution computed tomography (HRCT) scan of chest was done, in severe group 28 (80.0%) showed ground-glass opacity (GGO) and in non-severe group it was 41 (65.1%). The average duration of hospital stay was significantly higher in severe group (11.2 ± 8.1 days) than non-severe group (7.0 ± 5.9 days). Death was observed significantly higher in severe versus non-severe group (16.3% vs 1.4%) (p value <.05).
Conclusion: There was older age and male predominance in severe COVID -19 cases. Feritin, D-dimer were significantly higher in severe group and lower percentages of lymphocyte was also noticed in this group. The mortality and longer hospital stay were observed more in severe group than non-severe.
BIRDEM Med J 2022; 12(2): 129-135
Malignant hypercalcemia associated with ovarian dysgerminoma: a case report
Authors: Kudrat Ullah Md Amirul Islam , Khanam Fatisha , Ghosh Parijat , Binte Rouf Rushda Sharmin , Amin Mohammod Feroz 
Published: May 2022

Hypercalcemia is a well-recognized and potentially dangerous paraneoplastic manifestation of malignancy; furthermore, it is an important cancer related medical emergency. It has been endured in association with a number of malignancies; however, it is an unusual presentation of ovarian cancer like dysgerminoma. This neoplasm along with refractory hypercalcemia, which usually affects the young women, is a sign of advanced disease and indicates poor prognosis. In this report, we present a case of 31-year-old female, who presented with features of hypercalcemia which was associate with ovarian dysgerminoma.
BIRDEM Med J 2022; 12(2): 158-160
IMPLEMENTASI REVITALISASI PASAR TRADISIONAL B SRIKATON
Authors: Safari Muhammad Akip 
Published: May 2022

This study analyzes the implementation of the revitalization of Traditional Market B Srikaton based on the Regulation of Minister of Trade of the Republic of Indonesia on the assistant task and Guidelines on Construction and Facility Management Trade. This study uses a qualitative method descriptive, with a focus on the implementation of the revitalization of Traditional Market B Srikaton and model of revitalization as the fit in PERMENDAG No. 61 / M-DAG / PER / 8/2015 clause 13, paragraph 1 states that the development / revitalization of local markets as mentioned article 12, paragraph 2 shall be physical revitalization, management, socio-cultural and economic method of data collection in this study using observation, interview and documentation. Informants in the study is the apparatus of government agencies involved in the implementation of the revitalization, traders and buyers gained through observation and documentation. Implementation of the revitalization of traditional market B Srikaton going well. Model revitalization implemented in the revitalization of the market is the physical revitalization run in accordance with the purpose of revitalization such as construction 168 of 772 stall is not functioning. Revitalization management, socio-culture has not been fully achieved. The constraints faced in the implementation of the revitalization of the market is the development and empowerment of merchants have not done, yet the creation of a market environment that is conducive and comfortable. For economic revitalization are not achieved, due to decreased merchant income results after occupying / selling in stall of revitalization.
Segmentasi Citra Diaretdb1 Pada Area Hemorrhages Diabetic Retinopathy Menggunakan Metode Region Growing
Authors: Oktavia Fenty , Andreswari Desi , Susilo Boko 
Published: May 2022

Diabetic Retinophaty merupakan gangguan pembuluh darah di retina pada pasien yang mengidap Diabetes Melitus (Kencing Manis). Diabetic Retinopathy diklasifikasikan menjadi Nonproliferative Diabetic Retinopathy (NPDR) dan Proliverative Diabetic Retinopathy (PDR). Nonproliferative Diabetic Retinopathy ini dapat ditandai dengan keadaan mata yang mengandung unsur Microanerysms, Hemorrhages dan Eksudat, sedangkan Proliverative Diabetic Retinopathy adalah tahapan yang berada pada stadium lanjut yang menyebabkan gangguan penglihatan. Tujuan dari penelitian ini adalah mensegmentasi area Hemorrhages pada penyakit Diabetic Retinopathy dengan metode Region Growing, dan menentukan tingkat keberhasilan aplikasi dalam mensegmentasi Hemorrhages dalam menggunakan metode uji sensitivitas, spesifisitas dan akurasi. Pengujian pada penelitian ini menggunakan evaluasi performa berbasis objek yaitu mencari area Hemorrhages dengan cara mencari nilai piksel yang sama antara citra Groundtruth dan citra hasil aplikasi Matlab. Berdasarkan pengujian yang telah dilakukan pada sistem untuk segmentasi area Hemorrhages pada citra DIARETDB1 didapat rata-rata nilai sensitivitas = 8,82%, spesifisitas = 6,50% dan akurasi = 7,16%.
Desain dan Implementasi Aplikasi Manajemen Repository Surat Berbasis Web (Studi Kasus: Fakultas Teknik Universitas Bengkulu)
Authors: Yusa Mochammad , Coastera Funny Farady , Syahputra Wahyu , Hallatu Stefani Tasya 
Published: May 2022

Surat merupakan salah satu media informasi yang sering digunakan dalam menyampaikan sebuah informasi kepada orang lain. Pada lingkungan Fakultas Teknik ada beberapa kendala di bidang surat menyurat khususnya Surat Keputusan dan Surat Tugas. Pada Lingkungan Fakultas Teknik Universitas Bengkulu, pendistribuasian Surat Keputusan dan Surat Tugas masih dilakukan secara konvensional dengan menyerahkan langsung terhadap yang bersangkutan dalam bentuk hardcopy. Selain itu, SK dan Surat Tugas yang menumpuk juga sangat sulit untuk diurutkan berdasarkan tanggalnya sesuai dengan kebutuhan - kebutuhan penggunaan SK dan Surat Tugas tersebut. Penelusuran terkait resmi atau tidaknya SK dan Surat Tugas yang dikeluarkan juga menjadi kendala lainnya meskipun potensinya kecil namun tidak menutup kemungkinan hal ini dapat terjadi. Sehingga dilakukan penelitian ini yang bertujuan untuk merancang sistem informasi yang memungkinkan dapat mengarsipakan data histori surat keputusan dan surat tugas yang dikeluarkan dari Fakultas Teknik Universitas Bengkulu. Pembuatan sistem informasi ini menggunakan metode pengumpulan data dengan mengumpulkan data dari dokumen atau catatan yang ada, dan juga menggunakan metode studi literatur dengan menelusuri sumber – sumber tuliasan lainnya. Untuk mencapai hasil peneilitan ini dilakukan analisis masalah, kebutuhan, dan lainnya sehingga di dapatkan hasil perancangan UML dan perancangan sistem informasi beserta dengan pengujiannya.
Analisis Penetapan Skala Prioritas Penanganan Balita Stunting Menggunakan Metode DBSCAN Clustering (Studi Kasus Data Dinas Kesehatan Kabupaten Lebong)
Authors: Harjanto Taufik Dwi , Vatresia Arie , Faurina Ruvita 
Published: May 2022

Pada Peraturan Menteri Kesehatan Republik Indonesia Nomor 29 Tahun 2019 Tentang Penanggulangan Masalah Gizi Anak Akibat Penyakit Pada Pasal 2 dijelaskan bahwa pemerintah pusat dan pemerintah daerah bertanggung jawab terhadap penyelenggaraan penanggulangan masalah gizi bagi anak akibat penyakit dan diprioritaskan terhadap penyakit yang memerlukan upaya khusus untuk penyelamatan hidup dan mempunyai dampak terbesar pada angka kejadian stunting. Pada tahun 2017, pemerintah meluncurkan Rencana Aksi Nasional Kepresidenan yang bertujuan untuk mengatasi tingkat stunting yang tinggi di kalangan anak-anak di bawah usia 5 tahun. Rencana Aksi Nasional mengarahkan kementerian secara nasional untuk memfokuskan program dan kegiatan mereka pada tahun 2018 di 100 kabupaten dengan prevalensi stunting yang tinggi, insiden stunting dan tingkat kemiskinan yang tinggi Tujuan penelitian ini adalah Membangun suatu sistem yang menghasilkan pengelompokkan data fasilitas kesehatan berupa jumlah dokter, jumlah perawat, jumlah ahli gizi, jumlah posyandu, jumlah balita yang mendapatkan ASI ekslusif, jumlah balita yang mempunyai KIA/KMS, dan jumlah balita yang mendapatkan sarana air bersih skala prioritas masing-masing fasilitas kesehatan agar untuk mengetahui prioritas dari balita stunting menggunakan metode DBSCAN. Aplikasi ini dibuat dengan menggunakan Visual studio code dan R studio dengan bahasa pemrograman PHP dan Bahasa pemrograman R. Hasil dari penelitian ini berupa aplikasi pengelompokkan data dari fasilitas Kesehatan untuk menentukan skala prioritas untuk penanganan balita stunting . Pengujian software menggunakan metode Evaluasi model silhouette coefficient dan Black box. Berdasarkan hasil pengujian tersebut, secara dari epsilon 114 dan min pts 2 maka hasil dari silhouette coefficient adalah 0.51569473 dengan interpretasi bahwa klaster telah layak atau sesuai.
UPAYA PEMBERDAYAAN MASYARAKAT MELALUI PRODUKSI DAN PEMASARAN SUSU KEDELAI
Authors: Hartanti Dyah Yunita , Sutrawati Mimi 
Published: May 2022

Kegiatan pengabdian pada masyarakat (PPM) ini dilakukan dengan pemberdayaan masyarakat kelompok Tani Pelita Kusuma di Desa Bumi Harjo, Kecamatan Pinang Raya Kabupaten Bengkulu Utara. Tujuan dari kegiatan ini adalah untuk mengaktifkan kembali produksi susu kedelai yang sebelumnya terhenti karena beberapa kendala. Melalui kegiatan PPM ini diharapkan dapat memperbaiki kinerja kelompok Tani Pelita Kusuma dalam produksi, tata kelola keuangan, dan pemasaran. Program ini dilakukan melalui beberapa tahap, yakni 1) produksi susu kedelai siap saji; 2) transfer teknologi pengemasan susu kedelai; 3) Pemasaran produk dan aspek manajemen keuangan. Program kerja ini terlaksana dengan baik dengan pelatihan dan pendampingan anggota kelompok tani. Setelah kegiatan ini terjadi peningkatan kuantitas dan kualitas produksi olahan susu kedelai di kelompok Tani Pelita Kusuma. Pemasaran produk dan manajemen keuangan sangat penting untuk keberlanjutan usaha ini di masa yang akan datang. COMMUNITY EMPOWERMENT EFFORT THROUGH SOYMILK PRODUCTION AND MARKETING. This community service activity (PPM) is carried out by empowering the Pelita Kusuma farmer group community in Bumi Harjo Village, Pinang Raya District, North Bengkulu Regency. The purpose of this activity is to reactivate the production of soymilk which was previously stopped due to several obstacles. Through this PPM activity, it is expected to improve the performance of the Pelita Kusuma farmer group in production, financial management, and marketing. This program is carried out through several stages, namely 1) the production of ready-to-eat soy milk; 2) transfer of soy milk packaging technology; 3) Product marketing and financial management aspects. This work program is well implemented with the training and mentoring of farmer group members. After this activity, there was an increase in the quantity and quality of processed soymilk production in the Pelita Kusuma Farmer group.
Identifying Features of Cardiac Disease Phenotypes Based on Mechanical Function in a Catecholaminergic Polymorphic Ventricular Tachycardia Model
Authors: Stempien A. , Josvai M. , de Lange W. J. , Hernandez J. J. , Notbohm J. , Kamp T. J. , Valdivia H. H. , Eckhardt L. L. , Maginot K. R. , Ralphe J. C. , Crone W. C. 
Published: May 2022

Catecholaminergic polymorphic ventricular tachycardia (CPVT) is characterized by an arrhythmogenic mechanism involving disruption of calcium handling. This genetic disease can lead to sudden death in children and young adults during physical or emotional stress. Prior CPVT studies have focused on calcium handling, but mechanical functionality has rarely been investigated in vitro. In this research we combine stem cell-derived cardiomyocytes from a CPVT patient (RyR2-H2464D mutation) and a healthy familial control with an engineered culture platform to evaluate mechanical function of cardiomyocytes. Substrates with Young’s modulus ranging from 10 to 50 kPa were used in conjunction with microcontact printing of ECM proteins into defined patterns for subsequent attachment. Digital Image Correlation (DIC) was used to evaluate collections of contracting cells. The amplitude of contractile strain was utilized as a quantitative indicator of functionality and disease severity. We found statistically significant differences: the maximum contractile strain was consistently higher in patient samples compared to control samples on all substrate stiffnesses. Additionally, the patient cell line had a statistically significantly slower intrinsic contraction rate than the control, which agrees with prior literature. Differences in mechanical strain have not been previously reported, and hypercontractility is not a known characteristic of CPVT. However, functional changes can occur as the disease progresses, thus this observation may not represent behavior observed in adolescent and adult patients. These results add to the limited studies of mechanical function of CPVT CMs reported in literature and identify functional differences that should be further explored.
Mix and Match: Promoters and Terminators for Tuning Gene Expression in the Methylotrophic Yeast Ogataea polymorpha
Authors: Wefelmeier Katrin , Ebert Birgitta E. , Blank Lars M. , Schmitz Simone 
Published: May 2022

The yeast Ogataea polymorpha is an upcoming host for bio-manufacturing due to its unique physiological properties, including its broad substrate spectrum, and particularly its ability to utilize methanol as the sole carbon and energy source. However, metabolic engineering tools for O. polymorpha are still rare. In this study we characterized the influence of 6 promoters and 15 terminators on gene expression throughout batch cultivations with glucose, glycerol, and methanol as carbon sources as well as mixes of these carbon sources. For this characterization, a short half-life Green Fluorescent Protein (GFP) variant was chosen, which allows a precise temporal resolution of gene expression. Our promoter studies revealed how different promoters do not only influence the expression strength but also the timepoint of maximal expression. For example, the expression strength of the catalase promoter (pCAT) and the methanol oxidase promoter (pMOX) are comparable on methanol, but the maximum expression level of the pCAT is reached more than 24 h earlier. By varying the terminators, a 6-fold difference in gene expression was achieved with the MOX terminator boosting gene expression on all carbon sources by around 50% compared to the second-strongest terminator. It was shown that this exceptional increase in gene expression is achieved by the MOX terminator stabilizing the mRNA, which results in an increased transcript level in the cells. We further found that different pairing of promoters and terminators or the expression of a different gene (β-galactosidase gene) did not influence the performance of the genetic parts. Consequently, it is possible to mix and match promoters and terminators as independent elements to tune gene expression in O. polymorpha.
Anti-RAFLS Triterpenoids and Hepatoprotective Lignans From the Leaves of Tujia Ethnomedicine Kadsura heteroclita (Xuetong)
Authors: Wang Mengyun , Jiang Sai , Hussain Nusrat , Zafar Salman , Xie Qingling , Huang Feibing , Mao Linxi , Li Bin , Jian Yuqing , Wang Wei 
Published: May 2022

A pair of 3,4-seco-cycloartane triterpenoid isomers with a rare peroxy bridge, namely, xuetonins A and B (1 and 2), four new lignans xuetonlignans A–D (3–6), a new sesquiterpene xuetonpene (7), and a new natural product xuetonin C (8), along with 43 known compounds, were obtained from the leaves of Tujia ethnomedicine, Kadsura heteroclita. Their structures and configurations were determined with the help of a combination of 1D- and 2D-NMR, HRESIMS spectra, electronic circular dichroism (ECD), and X-ray diffraction data. Compounds 2, 10, 13–15, and 17–19 showed moderate-to-potent activity against rheumatoid arthritis fibroblast-like synoviocytes (RAFLS) with IC50 values of 19.81 ± 0.26, 12.73 ± 0.29, 5.70 ± 0.24, 9.25 ± 0.79, 5.66 ± 0.52, 11.91 ± 0.44, 13.22 ± 0.27, and 15.94 ± 0.36 μM, respectively. Furthermore, compounds 22, 25, and 31 exhibited significant hepatoprotective effects against N-acetyl-p-aminophenol (APAP)–induced toxicity in HepG2 cells at 10 μM, and the cell viability increased by 12.93, 25.23, and 13.91%, respectively, compared with that in the model group (cf. bicyclol, 12.60%).
Fractal Geometry Illustrated Left Atrial Appendage Morphology That Predicted Thrombosis and Stroke in Patients With Atrial Fibrillation
Authors: Lei Chuxiang , Gao Qi , Wei Runjie , Li Qijie , Liu Xingli , Wu Lingmin , Yao Yan , Fan Hongguang , Zheng Zhe 
Published: May 2022

BackgroundThis study aims to correlate the morphological complexity of left atrial appendage (LAA) with thrombosis and stroke in patients with atrial fibrillation (AF).MethodsThe training cohort consisted of 46 patients with AF (age 55.8 ± 7.2 years, 73.9% men) who were referred for radiofrequency catheter ablation. An independent validation cohort consisting of 443 patients with AF was enrolled for further verification. All patients in the training cohort underwent both transesophageal echocardiography (TEE) and enhanced computed tomography (CT). Fractal dimension (FD) analysis was performed to evaluate the morphological complexity of LAAs quantitatively. Clinical and imaging manifestations, FD of LAAs, and diagnostic accuracy were investigated and compared between patients with AF in both training and validation cohorts.ResultsIn the training cohort, LAAs (n = 22) with thrombi had significantly higher FD than those without thrombi (n = 24) h 0.44 ± 0.07 vs. 2.35 ± 0.11, p = 0.003). Receiver-operating characteristic (ROC) analysis suggested that the diagnostic accuracy of FD combined with a CHA2DS2-VaSc score was significantly higher than that of the CHA2DS2-VaSc score alone in low- to moderate-risk patients with AF (area under the curve 0.8479 vs. 0.6958, p = 0.009). The results were also validated in an independent external validation cohort and demonstrated that increased FD was associated with stroke. Hemodynamic analysis revealed that LAAs with thrombi and high FD were prone to blood stasis and lower blood flow rate.ConclusionLAA morphological complexity is closely associated with thrombosis and stroke in patients with paroxysmal AF. A new risk assessment system combining CHA2DS2-VaSc score and FD has a higher diagnostic accuracy in predicting LAA thrombosis.
Alcohol Abuse Associated With Increased Risk of Angiographic Vasospasm and Delayed Cerebral Ischemia in Patients With Aneurysmal Subarachnoid Hemorrhage Requiring Mechanical Ventilation
Authors: Zhao Lei , Cheng Chao , Peng Liwei , Zuo Wei , Xiong Dong , Zhang Lei , Mao Zilong , Zhang Jin'an , Wu Xia , Jiang Xue , Wang Peng , Li Weixin 
Published: May 2022

ObjectiveAlthough alcohol abuse has been indicated to cause cerebral aneurysm development and rupture, there is limited data on the impact of alcohol abuse on outcomes after an aneurysmal subarachnoid hemorrhage (aSAH). This study aims to investigate whether alcohol abuse increases the risk of angiographic vasospasm and delayed cerebral ischemia (DCI) in critically ill patients with aSAH.MethodsWe conducted a secondary analysis based on a retrospective study in a French university hospital intensive care unit (ICU). Patients with aSAH requiring mechanical ventilation hospitalized between 2010 and 2015 were included. Patients were segregated according to alcohol abuse (yes or no). Multivariable logistic regression analysis was used to identify the independent risk factors associated with angiographic vasospasm and DCI.ResultsThe patient proportion of alcohol abuse was dramatically greater in males than that in females (p &lt; 0.001). The Simplified Acute Physiology Score II (SAPSII) score on admission did not show a statistical difference. Neither did the World Federation of Neurosurgical Societies (WFNS) and Fisher scores. Patients with alcohol abuse were more likely to develop angiographic vasospasm (OR 3.65, 95% CI 1.17–11.39; p = 0.0260) and DCI (OR 3.53, 95% CI 1.13–10.97; p = 0.0294) as evidenced by multivariable logistic regression analysis.ConclusionsIn this study, patients with alcohol abuse are at higher odds of angiographic vasospasm and DCI, which are related to poor prognosis following aSAH. These findings are important for the prevention and clinical management of aSAH.