bluuebunny/crossref_metadata_2025_split huggingface dataset:
Submesoscale Coherent Vortices Observed in the Northeastern South China Sea
Authors: Zhang Xincheng , Zhang Zhiwei , McWilliams James C. , Sun Zhongbin , Zhao Wei , Tian Jiwei 
Published: January 2022

AbstractAlthough submesoscale coherent vortices (SCVs) have been observed in different parts of the world's oceans, most of them were captured by accident by a limited number of hydrographic profiles. Here, using concurrent velocity and temperature/salinity measurements from a submesoscale‐resolving mooring array (2 km), two oppositely rotating SCVs are for the first time reported in detail in the northeastern South China Sea (NESCS). For the anticyclonic (cyclonic) SCV, its core lays at 210 m (180 m) with a mean Rossby number of −0.76 (0.65); its maximum swirl velocity, radius, vertical scale, and Burger number are estimated to be 0.43 m s−1, 31 ± 5 km, 235 m, and 1.5 (0.23 m s−1, 16 ± 3 km, 100 m, and 2.5), respectively. Corresponding to the SCVs' submesoscale nature, their momentum is governed by gradient‐wind rather than geostrophic balance. Consequently, kinetic energy of the anticyclonic (cyclonic) SCV would be on average underestimated (overestimated) by 47% (68%) if the traditional geostrophic relation was used to diagnose the velocity. Further analysis shows that thermohaline properties within the anticyclonic and cyclonic SCVs are close to the Kuroshio water and the local NESCS water, respectively. By combing water mass tracing and high‐resolution simulations, we suggested that the anticyclonic (cyclonic) SCV was generated in the southern Luzon Strait (southwest of Taiwan) through current‐topography interaction. We further proposed that the observed SCVs here should be a common phenomenon in the NESCS, which may provide a novel route for the tracer exchange between the NESCS and the western Pacific.
Solar Wind—Magnetosphere Coupling Functions: Pitfalls, Limitations, and Applications
Authors: Lockwood Mike 
Published: January 2022

AbstractSolar wind‐magnetosphere coupling functions have now been in use for almost 50 years. In that time, a very large number of formulations have been proposed. As they become increasingly subsumed into systems analysis and machine‐learning studies of the magnetosphere, it is timely to establish best practice in their derivation and study their limitations. This paper reports on a number of studies carried out to establish some key points. Particular attention is paid to the best metric used to evaluate their performance and how it depends on the application for which the coupling function is intended.
MXene supported surface plasmons on telecommunications optical fibers
Authors: Pacheco-Peña Victor , Hallam Toby , Healy Noel 
Published: January 2022

AbstractMXenes, an emerging class of two-dimensional materials, exhibit characteristics that promise significant potential for their use in next generation optoelectronic sensors. An interplay between interband transitions and boundary effects offer the potential to tune the plasma frequencies over a large spectral range from the near-infrared to the mid-infrared. This tuneability along with the ‘layered’ nature of the material not only offer the flexibility to produce plasmon resonances across a wide range of wavelengths, but also add a degree of freedom to the sensing mechanism by allowing the plasma frequency to be modulated. Here we show, numerically, that MXenes can support plasmons in the telecommunications frequency range and that surface plasmon resonances can be excited on a standard MXene coated side polished optical fiber. Thus, presenting the tantalising prospect of highly selective distributed optical fiber sensor networks.
Mechanical memory operations in piezotransistive GaN microcantilevers using Au nanoparticle-enhanced photoacoustic excitation
Authors: Bayram Ferhat , Gajula Durga , Khan Digangana , Koley Goutam 
Published: January 2022

AbstractNonlinear oscillations in micro- and nanoelectromechanical systems have emerged as an exciting research area in recent years due to their promise in realizing low-power, scalable, and reconfigurable mechanical memory and logic devices. Here, we report ultralow-power mechanical memory operations utilizing the nonlinear oscillation regime of GaN microcantilevers with embedded piezotransistive AlGaN/GaN heterostructure field effect transistors as highly sensitive deflection transducers. Switching between the high and low oscillatory states of the nonlinear oscillation regime was demonstrated using a novel phase-controlled opto-mechanical excitation setup, utilizing a piezo actuator and a pulsed laser as the primary and secondary excitation sources, respectively. Laser-based photoacoustic excitation was amplified through plasmonic absorption in Au nanoparticles deposited on a transistor. Thus, the minimum switching energy required for reliable memory operations was reduced to less than a picojoule (pJ), which translates to one of the lowest ever reported, when normalized for mass.
Socioeconomic status, BMI, and brain development in children
Authors: Dennis Evan , Manza Peter , Volkow Nora D. 
Published: January 2022

AbstractLow socioeconomic status (SES) in childhood is associated with deficits in executive function and changes in cortical morphology. Furthermore, rates of childhood obesity are greater among low SES children and childhood obesity is also associated with cortical alterations and impaired neurocognition, specifically in the domain of executive function. To investigate the influence of BMI on the relationships between SES and both neurocognition and brain morphology, we used data from the Adolescent Brain Cognitive Development (ABCD) study to construct multiple linear regression models and conduct mediation analyses. Overall, SES as measured by household income, highest level of parental education, and area deprivation, was associated with lower BMI, greater total and prefrontal cortical volume, and better performance on assessments of executive function. Mediation analysis indicated that BMI had a significant indirect effect on associations between area deprivation and both total and prefrontal cortical volumes. BMI also played a mediating role in the associations between area deprivation and composite neurocognitive scores, which were driven by performance on tasks of working memory and cognitive flexibility, but not cognitive control. These findings suggest that BMI should be considered in future studies investigating the relationship between low SES and poor neurodevelopmental outcomes.
Loss of NEDD4 causes complete XY gonadal sex reversal in mice
Authors: Windley Simon P. , Mayère Chloé , McGovern Alice E. , Harvey Natasha L. , Nef Serge , Schwarz Quenten , Kumar Sharad , Wilhelm Dagmar 
Published: January 2022

AbstractGonadogenesis is the process wherein two morphologically distinct organs, the testis and the ovary, arise from a common precursor. In mammals, maleness is driven by the expression of Sry. SRY subsequently upregulates the related family member Sox9 which is responsible for initiating testis differentiation while repressing factors critical to ovarian development such as FOXL2 and β-catenin. Here, we report a hitherto uncharacterised role for the ubiquitin-protein ligase NEDD4 in this process. XY Nedd4-deficient mice exhibit complete male-to-female gonadal sex reversal shown by the ectopic upregulation of Foxl2 expression at the time of gonadal sex determination as well as insufficient upregulation of Sox9. This sex reversal extends to germ cells with ectopic expression of SYCP3 in XY Nedd4-/- germ cells and significantly higher Sycp3 transcripts in XY and XX Nedd4-deficient mice when compared to both XY and XX controls. Further, Nedd4-/- mice exhibit reduced gonadal precursor cell formation and gonadal size as a result of reduced proliferation within the developing gonad as well as reduced Nr5a1 expression. Together, these results establish an essential role for NEDD4 in XY gonadal sex determination and development and suggest a potential role for NEDD4 in orchestrating these cell fate decisions through the suppression of the female pathway to ensure proper testis differentiation.
Galectin-1 confers resistance to doxorubicin in hepatocellular carcinoma cells through modulation of P-glycoprotein expression
Authors: Carabias Pablo , Espelt María V. , Bacigalupo María L. , Rojas Paola , Sarrias Luciana , Rubin Ayelén , Saffioti Nicolás A. , Elola María T. , Rossi Juan P. , Wolfenstein-Todel Carlota , Rabinovich Gabriel A. , Troncoso María F. 
Published: January 2022

AbstractGalectin-1 (GAL1), a β-galactoside-binding protein abundantly expressed in the tumor microenvironment, has emerged as a key mechanism of chemoresistance developed by different tumors. Although increased expression of GAL1 is a hallmark of hepatocellular carcinoma (HCC) progression, aggressiveness and metastasis, limited information is available on the role of this endogenous lectin in HCC resistance to chemotherapy. Moreover, the precise mechanisms underlying this effect are uncertain. HCC has evolved different mechanisms of resistance to chemotherapy including those involving the P-glycoprotein (P-gp), an ATP-dependent drug efflux pump, which controls intracellular drug concentration. Here, we investigated the molecular mechanism underlying GAL1-mediated chemoresistance in HCC cells, particularly the involvement of P-gp in this effect. Our results show that GAL1 protected HepG2 cells from doxorubicin (DOX)- and sorafenib-induced cell death in vitro. Accordingly, GAL1-overexpressing HepG2 cells generated DOX-resistant tumors in vivo. High expression of GAL1 in HepG2 cells reduced intracellular accumulation of DOX likely by increasing P-gp protein expression rather than altering its membrane localization. GAL1-mediated increase of P-gp expression involved activation of the phosphatidylinositol-3 kinase (PI3K) signaling pathway. Moreover, ‘loss-of-function’ experiments revealed that P-gp mediates GAL1-driven resistance to DOX, but not to sorafenib, in HepG2 cells. Conversely, in PLC/PRF/5 cells, P-gp protein expression was undetectable and GAL1 did not control resistance to DOX or sorafenib, supporting the critical role of P-gp in mediating GAL1 effects. Collectively, our findings suggest that GAL1 confers chemoresistance in HCC through mechanisms involving modulation of P-gp, thus emphasizing the role of this lectin as a potential therapeutic target in HCC.
Advances in the pathogenesis of psoriasis: from keratinocyte perspective
Authors: Zhou Xue , Chen Youdong , Cui Lian , Shi Yuling , Guo Chunyuan 
Published: January 2022

AbstractPsoriasis is a complex long-lasting inflammatory skin disease with high prevalence and associated comorbidity. It is characterized by epidermal hyperplasia and dermal infiltration of immune cells. Here, we review the role of keratinocytes in the pathogenesis of psoriasis, focusing on factors relevant to genetics, cytokines and receptors, metabolism, cell signaling, transcription factors, non-coding RNAs, antimicrobial peptides, and proteins with other different functions. The critical role of keratinocytes in initiating and maintaining the inflammatory state suggests the great significance of targeting keratinocytes for the treatment of psoriasis.
E2F transcription factor 2-activated DLEU2 contributes to prostate tumorigenesis by upregulating serum and glucocorticoid-induced protein kinase 1
Authors: Li Peizhang , Xu Huan , Yang Liu , Zhan Ming , Shi Yuanping , Zhang Caoxu , Gao Dajun , Gu Meng , Chen Yanbo , Wang Zhong 
Published: January 2022

AbstractLong noncoding RNAs (lncRNAs) participate in biological processes in multiple types of tumors. However, the regulatory patterns of lncRNAs in prostate cancer remain largely unclear. Here, we evaluated the expression and roles of the lncRNA DLEU2 in prostate cancer. Our results showed that DLEU2 was upregulated in advanced prostate cancer tissues. Patients with prostate cancer displaying high expression of DLEU2 had a poor prognosis. Moreover, we demonstrated that overexpression of DLEU2 facilitated the proliferation, migration, and invasion of prostate cancer in vitro. Mechanistically, DLEU2 promoted serum and glucocorticoid-induced protein kinase 1 (SGK1) expression by acting as an miR-582-5p sponge, and the transcription of DLEU2 was activated by the dysregulation of E2F transcription factor 2 (E2F2) expression in prostate cancer. Furthermore, knockdown of DLEU2 attenuated prostate cancer tumorigenesis in vivo. Notably, these findings suggested that E2F2-activated DLEU2 may function as a competing endogenous RNA to facilitate prostate cancer progression by targeting the miR-582-5p/SGK1 axis.
TRIP13, identified as a hub gene of tumor progression, is the target of microRNA-4693-5p and a potential therapeutic target for colorectal cancer
Authors: Chen Yan , Chen Danqi , Qin Ying , Qiu Cheng , Zhou Yaoyao , Dai Mengmeng , Li Lulu , Sun Qinsheng , Jiang Yuyang 
Published: January 2022

AbstractColorectal cancer (CRC) is one of the digestive tract malignancies whose early symptoms are not obvious. This study aimed to identify novel targets for CRC therapy, especially early-stage CRC, by reanalyzing the publicly available GEO and TCGA databases. Thyroid hormone receptor interactor 13 (TRIP13) correlated with tumor progression and prognosis of patients after several rounds of analysis, including weighted gene correlation network analysis (WGCNA), and further chosen for experimental validation in cancer cell lines and patient samples. We identified that mRNA and protein levels of TRIP13 increased in CRC cells and tumor tissues with tumor progression. miR-4693-5p was significantly downregulated in CRC tumor tissues and bound to the 3′ untranslated region (3′UTR) of TRIP13, downregulating TRIP13 expression. DCZ0415, a small molecule inhibitor targeting TRIP13, induced anti-tumor activity in vitro and in vivo. DCZ0415 markedly suppressed CRC cell proliferation, migration, and tumor growth, promoted cell apoptosis, and resulted in the arrest of the cell cycle. Our research suggests that TRIP13 might play a crucial role in CRC progression and could be a potential target for CRC therapy.
The sensitivity of simulated streamflow to individual hydrologic processes across North America
Authors: Mai Juliane , Craig James R. , Tolson Bryan A. , Arsenault Richard 
Published: January 2022

AbstractStreamflow sensitivity to different hydrologic processes varies in both space and time. This sensitivity is traditionally evaluated for the parameters specific to a given hydrologic model simulating streamflow. In this study, we apply a novel analysis over more than 3000 basins across North America considering a blended hydrologic model structure, which includes not only parametric, but also structural uncertainties. This enables seamless quantification of model process sensitivities and parameter sensitivities across a continuous set of models. It also leads to high-level conclusions about the importance of water cycle components on streamflow predictions, such as quickflow being the most sensitive process for streamflow simulations across the North American continent. The results of the 3000 basins are used to derive an approximation of sensitivities based on physiographic and climatologic data without the need to perform expensive sensitivity analyses. Detailed spatio-temporal inputs and results are shared through an interactive website.
Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediate-risk human prostate cancer
Authors: Sushentsev Nikita , McLean Mary A. , Warren Anne Y. , Benjamin Arnold J. V. , Brodie Cara , Frary Amy , Gill Andrew B. , Jones Julia , Kaggie Joshua D. , Lamb Benjamin W. , Locke Matthew J. , Miller Jodi L. , Mills Ian G. , Priest Andrew N. , Robb Fraser J. L. , Shah Nimish , Schulte Rolf F. , Graves Martin J. , Gnanapragasam Vincent J. , Brindle Kevin M. , Barrett Tristan , Gallagher Ferdia A. 
Published: January 2022

AbstractHyperpolarised magnetic resonance imaging (HP 13C-MRI) is an emerging clinical technique to detect [1-13C]lactate production in prostate cancer (PCa) following intravenous injection of hyperpolarised [1-13C]pyruvate. Here we differentiate clinically significant PCa from indolent disease in a low/intermediate-risk population by correlating [1-13C]lactate labelling on MRI with the percentage of Gleason pattern 4 (%GP4) disease. Using immunohistochemistry and spatial transcriptomics, we show that HP 13C-MRI predominantly measures metabolism in the epithelial compartment of the tumour, rather than the stroma. MRI-derived tumour [1-13C]lactate labelling correlated with epithelial mRNA expression of the enzyme lactate dehydrogenase (LDHA and LDHB combined), and the ratio of lactate transporter expression between the epithelial and stromal compartments (epithelium-to-stroma MCT4). We observe similar changes in MCT4, LDHA, and LDHB between tumours with primary Gleason patterns 3 and 4 in an independent TCGA cohort. Therefore, HP 13C-MRI can metabolically phenotype clinically significant disease based on underlying metabolic differences in the epithelial and stromal tumour compartments.
Viral infection and transmission in a large, well-traced outbreak caused by the SARS-CoV-2 Delta variant
Authors: Li Baisheng , Deng Aiping , Li Kuibiao , Hu Yao , Li Zhencui , Shi Yaling , Xiong Qianling , Liu Zhe , Guo Qianfang , Zou Lirong , Zhang Huan , Zhang Meng , Ouyang Fangzhu , Su Juan , Su Wenzhe , Xu Jing , Lin Huifang , Sun Jing , Peng Jinju , Jiang Huiming , Zhou Pingping , Hu Ting , Luo Min , Zhang Yingtao , Zheng Huanying , Xiao Jianpeng , Liu Tao , Tan Mingkai , Che Rongfei , Zeng Hanri , Zheng Zhonghua , Huang Yushi , Yu Jianxiang , Yi Lina , Wu Jie , Chen Jingdiao , Zhong Haojie , Deng Xiaoling , Kang Min , Pybus Oliver G. , Hall Matthew , Lythgoe Katrina A. , Li Yan , Yuan Jun , He Jianfeng , Lu Jing 
Published: January 2022

AbstractThe SARS-CoV-2 Delta variant has spread rapidly worldwide. To provide data on its virological profile, we here report the first local transmission of Delta in mainland China. All 167 infections could be traced back to the first index case. Daily sequential PCR testing of quarantined individuals indicated that the viral loads of Delta infections, when they first become PCR-positive, were on average ~1000 times greater compared to lineage A/B infections during the first epidemic wave in China in early 2020, suggesting potentially faster viral replication and greater infectiousness of Delta during early infection. The estimated transmission bottleneck size of the Delta variant was generally narrow, with 1-3 virions in 29 donor-recipient transmission pairs. However, the transmission of minor iSNVs resulted in at least 3 of the 34 substitutions that were identified in the outbreak, highlighting the contribution of intra-host variants to population-level viral diversity during rapid spread.
Structural basis of the ligand binding and signaling mechanism of melatonin receptors
Authors: Wang Qinggong , Lu Qiuyuan , Guo Qiong , Teng Maikun , Gong Qingguo , Li Xu , Du Yang , Liu Zheng , Tao Yuyong 
Published: January 2022

AbstractMelatonin receptors (MT1and MT2in humans) are family A G protein–coupled receptors that respond to the neurohormone melatonin to regulate circadian rhythm and sleep. Numerous efforts have been made to develop drugs targeting melatonin receptors for the treatment of insomnia, circadian rhythm disorder, and cancer. However, designing subtype-selective melatonergic drugs remains challenging. Here, we report the cryo-EM structures of the MT1–Gisignaling complex with 2-iodomelatonin and ramelteon and the MT2–Gisignaling complex with ramelteon. These structures, together with the reported functional data, reveal that although MT1and MT2possess highly similar orthosteric ligand-binding pockets, they also display distinctive features that could be targeted to design subtype-selective drugs. The unique structural motifs in MT1and MT2mediate structural rearrangements with a particularly wide opening on the cytoplasmic side. Giis engaged in the receptor core shared by MT1and MT2and presents a conformation deviating from those in other Gicomplexes. Together, our results provide new clues for designing melatonergic drugs and further insights into understanding the G protein coupling mechanism.
Genetic markers for non-syndromic orofacial clefts in populations of European ancestry: a meta-analysis
Authors: Slavec Lara , Karas Kuželički Nataša , Locatelli Igor , Geršak Ksenija 
Published: January 2022

AbstractTo date, the involvement of various genetic markers in the aetiopathogenesis of non-syndromic orofacial cleft (nsOFC) has been extensively studied. In the present study, we focused on studies performed on populations of European ancestry to systematically review the available literature to define relevant genetic risk factors for nsOFC. Eligible studies were obtained by searching Ovid Medline and Ovid Embase. We gathered the genetic markers from population-based case–control studies on nsOFC, and conducted meta-analysis on the repeatedly reported markers. Whenever possible, we performed stratified analysis based on different nsOFC phenotypes, using allelic, dominant, recessive and overdominant genetic models. Effect sizes were expressed as pooled odds ratios (ORs) with 95% confidence intervals (CIs), and p ≤ 0.05 were considered statistically significant. A total of 84 studies were eligible for this systematic review, with > 700 markers included. Of these, 43 studies were included in the meta-analysis. We analysed 47 genetic variants in 30 genes/loci, which resulted in 226 forest plots. There were statistically significant associations between at least one of the nsOFC phenotypes and 19 genetic variants in 13 genes/loci. These data suggest that IRF6, GRHL3, 8q24, VAX1, TGFA, FOXE1, ABCA4, NOG, GREM1, AXIN2, DVL2, WNT3A and WNT5A have high potential as biomarkers of nsOFC in populations of European descent. Although other meta-analyses that included European samples have been performed on a limited number of genetic variants, this study represents the first meta-analysis of all genetic markers that have been studied in connection with nsOFC in populations of European ancestry.
Shifts in the foraging tactics of crocodiles following invasion by toxic prey
Authors: Aiyer Abhilasha , Shine Richard , Somaweera Ruchira , Bell Tina , Ward-Fear Georgia 
Published: January 2022

AbstractBiological invasions can modify the behaviour of vulnerable native species in subtle ways. For example, native predators may learn or evolve to reduce foraging in conditions (habitats, times of day) that expose them to a toxic invasive species. In tropical Australia, freshwater crocodiles (Crocodylus johnstoni) are often fatally poisoned when they ingest invasive cane toads (Rhinella marina). The risk may be greatest if toads are seized on land, where a predator cannot wash away the toxins before they are absorbed into its bloodstream. Hence, toad invasion might induce crocodiles to forage in aquatic habitats only, foregoing terrestrial hunting. To test this idea, we conducted standardised trials of bait presentation to free-ranging crocodiles in sites with and without invasive toads. As anticipated, crocodiles rapidly learned to avoid consuming toads, and shifted to almost exclusively aquatic foraging.