bluuebunny/crossref_metadata_2025_split huggingface dataset:
The characteristics of nano-micron calcite particles / common bacteria complex and its interfacial interaction
Authors: Zhao Yulian , Yang Yujie , Dong Faqin , Dai Qunwei 
Published: October 2022

Abstract

Suspended particulate in the atmosphere are composite pollutants composed of minerals, heavy metal ions, organic pollutants and microorganisms, etc. Minerals can provide shelter for living microorganisms, and forming a mineral / microorganism complex. In this paper, the nano-micron calcite particles / bacteria complex was taken as the research object, the morphology, particle size, surface electrical properties, surface groups and other characteristics of the mineral / bacteria complex were explored by modern analysis and testing methods, focusing on the analysis of the interface between mineral and bacteria in the complex. The SEM / CLSM results showed that the morphology of the complex was characterized by bacteria adhering to the surface of large particles of mineral or the aggregates of nano-mineral, and single nano-mineral particles adhering to the end and surface of bacteria. The particle size of the complex was about 2.07 ~ 192.4 times larger than that of the original mineral particles, and the particle size of nano CaCO3 / bacteria complex was larger than that of calcite / bacteria complex. The surface potential of calcite / bacteria complex is between mineral and bacteria, while the surface potential of nano CaCO3 / bacteria complex approaches mineral particles. The FTIR spectrum of mineral / bacteria complex was mainly based on the infrared characteristics of minerals, accompanied by the infrared characteristics of bacteria. Spectroscopic analysis of the secondary structure of bacterial membrane proteins in the complex showed that the calcite / bacteria complex was dominated by electrostatic attraction. The β-fold / α-helix ratio of bacteria in the mineral / Staphylococcus aureus complex increased, indicating that the secondary structure of bacteria surface proteins was more stable and the hydrogen bond effect was strong.
Colletotrichum acericola sp. nov. from seeds of invasive alien Acer negundo in Poland: delayed pathogen introduction after its host appearance?
Authors: Patejuk Katarzyna , Czachura Paweł , Baturo-Cieśniewska Anna , Owczarek-Kościelniak Magdalena , Pusz Wojciech , Najberek Kamil , Piątek Marcin 
Published: October 2022

Abstract
The boxelder maple (Acer negundo, Sapindaceae) is one of the most common tree species of the genus Acer natively growing in North America. In the 17th century A. negundo was introduced to Europe as an ornamental plant, and from this point forward it started uncontrolled expansion on new territories, as an alien invasive species (IAS), threatening local ecosystems and human’s safeness. Etiological symptoms of fungal development on boxelders’ seeds and petioles, were observed in 2017 and 2021 in the municipality of Wrocław (SW Poland, Europe), outside of host native occurrence. Morphological analyses combined with multilocus phylogenetic analyses (using rDNA ITS, gapdh, act, tub2, chs-1, and his3 sequences), indicated that isolated strains belong to a new species of Colletotrichum, from the rare Colletotrichum agaves species complex. Name Colletotrichum acericola is proposed for this fungus, after its host on which it was found. It is the second species of the Colletotrichum agaves species complex described from the dicotyledonous host and outside host plants of the family Asparagaceae. Based on phylogenetic analyses and deposited in NCBI-GenBank environmental sequences (ex endophytes of Dalea purpurea and Panicum virgatum in prairies of USA) assigned here to this species, it is likely that Colletotrichum acericola is native to North America and introduced to Europe after its host plant appearance, maybe on nursery material. The possible future invasion of C. acericola on Acer negundo and its spillover to other (native) Acer species in Europe are discussed.
Strategies to Fight Disinformation in Health: Is It Possible for a Brazilian Podcast to Reach Any Target Audience?
Authors: Silva Iorana Candido da , Nogueira Maria Rayssa do Nascimento , de Oliveira Carlos Henrique , Santos Marcos Nascimento dos , Santos Vania dos , NICOLETE Larissa Deadame de Figueiredo 
Published: October 2022

Abstract
Purpose
Knowing the use in the education of podcasts was occurring in some countries, the present work developed one in Portuguese. The aim was to promote health and combat disinformation in Brazil.
Methods
Different categories of Podcasts were created: shorter/longer; with/without guests and disease-related issues or other topics about prevention/health education. After that, the audios were edited and submitted on platforms: It was analyzed through the data generated by the published episodes.
Results
It was observed that duration vs several reproductions did not correlate with (p = 0.2521). Then, the presence/absence of guests (p = 0.1779). However, themes related to infectious and chronic diseases obtained more significant results (p = 0.0466) when compared to the other topics. Also, we noticed that our listeners are primarily men aged between 18 and 27 years old and residing in Brazil.
Conclusion
Podcasts may be able to disseminate knowledge, however, themes and the type of audience must be considered to choose this kind of approach.
Associations of the age of menarche and menopause with hypertension in menopausal women in Rasht, Iran in 2020-2021
Authors: Ahmadnia Zahra , Salari Arsalan , Borghei Yasaman , Mousavi Seyyed Aboozar Fakhr- , Mousavi Seyyed Mehdi , Rafiei Elahe , Karami Samaneh 
Published: October 2022

Abstract
Background
A woman's age of menarche as well as her age of menopause are two important points in her reproductive life. Furthermore, hypertension (HTN) is one of the important causes of cardiovascular diseases (CVD) as well as death, and has been considered as a public health issue all around the world. The current study aims at investigating the association of the age of menarche and menopause with HTN in menopausal women.
Methods
The participants who took part in this cross-sectional study included 1500 postmenopausal women. Baecke Habitual Physical Activity Questionnaire (BHPAQ), blood sample and blood pressure (BP) data were analyzed. The researchers examined the association using logistic regression. Data analysis was conducted using SPSS v.21 software. The significance level was considered P<0.05 for all the tests.
Results
Results was seen between the age of menarche and systolic BP (Pearson's Correlation Coefficient, r=0.086, p=0.001). No significant relationship was observed between the age of menarche and menopause with the chance of high BP in postmenopausal women. A significant relationship was reported between the age of menarche and systolic BP in postmenopausal women. The level of cholesterol (P<o/oo1) and LDL (P<o/oo1) had a positive effect on increasing systolic BP. However, FBS had a negative effect on increasing BP.
Conclusions
These results suggested that the associations of age at menarche with hypertension could be reduced by factors related to place of residence in Iran and knowledge of the association between age at menarche and living in urban areas, number of children, cholesterol levels, LDL, and FBS in women help prevent the development of hypertension.
Should ICSI be implemented on patients with poor-quality embryos in the previous IVF cycle?
Authors: Wang Jiang , Xiong Shun , Xia Fei , Zhu Jiahong , Liao Haiyuan , Wei Biao , Zou Jiayi , Han Wei 
Published: October 2022

Abstract
Purpose
to evaluate whether ICSI can improve the quality of embryo in patients who had their previous IVF cycle cancelled for poor-quality embryo.
Methods
This retrospective cohort study compared the embryo quality and clinical outcome following intracytoplasmic sperm injection (ICSI) and conventional in-vitro fertilization (IVF) in patients who had their previous IVF cycle cancelled for poor-quality embryo.
Results
According to the inclusion criteria, a total of 336 ovarian stimulation cycles were performed in the reproductive medical center of Chongqing Health Center for Women and Children Hospital from March 2016 to June 2022. During this period, ICSI and conventional IVF methods were applied in 158 and 178 cycles, respectively. Apart from the number of high-quality embryos, there were no notable differences in patients baseline characteristics and other laboratory results in the two groups. Compared with IVF, ICSI resulted in a significantly higher high-quality embryo rate (6.21% vs. 2.69%, P < 0.05). Among the 226 embryo transfer cycles, there were no notable differences in miscarriage rate and implantation rate in IVF and ICSI group. Moreover, the clinical pregnancy rate was significantly higher in the ICSI group than those in IVF group (56.52% vs. 43.24%, p < 0.05), and implantation rate seemed to increase in the ICSI group (41.58 vs. 34.64%, p > 0.05%);
Conclusion
The present study suggested that ICSI cannot increase the available embryo number rate in patients without any available embryos after normal fertilization in the previous IVF cycle, however, it can significantly improve the quality of embryo and the clinical pregnancy.
Experimental study of flow pattern in rectangular fish breeding ponds
Authors: Naghizadeh Mahdi , Moghbeli Atefeh , Zounemat-Kermani Mohammad 
Published: October 2022

Abstract

The current research investigates the flow pattern effect on
the performance of rectangular fish breeding ponds based on
hydraulic efficiency and stagnant regions indicators. Three
different flow patterns (linear, vortex cells with rotation in
opposite directions, and vortex cells with rotation in one
direction) with various inlet flow momentum forces (0.88N, 0.022N,
0.01N, 0.005N, and 0.003N) were investigated in the present
experimental study. A rectangular pond with dimensions of 200×50×13
Cm was also used to conduct the experiments. The results obtained
from the flow pattern experimental modelling show the more
appropriate performance of the hydraulic condition in flow pattern
vortex cells with rotation in opposite directions and vortex cells
with rotation in one direction, which were formed by redesign1 and
redesign2. The findings from the research show that the linear flow
pattern with tested momentum forces does not result in optimal
hydraulic efficiency (λ > 0.75). If the vortex cells with
rotation in one direction and the vortex cells with rotation in the
opposite flow patterns replace with the linear flow pattern,
hydraulic efficiency and stagnant regions are optimized in
rectangular ponds
Clinical Value of 18F-FAPI PET/CT in assessing early-stage fibrosis of graft after liver transplantation: preliminary experience
Authors: Fang Xiaohan , Xie Man , Zhao Youwei , Wang Ye , Zhang Qun , Tian Qiuju , Zhao Liu , Zhang Bei , Wei Zhimin , Kong Xinjuan , Yang Guangjie , Cai Jinzhen , Rao Wei 
Published: October 2022

Abstract
Aims
To investigate the clinical usefulness and performance of 18F-FAPI PET/CT in assessing early-stage liver fibrosis in liver transplantation (LT) recipients.
Methods
A prospective study including 17 LT recipients and 12 chronic Hepatitis B (CHB) patients was conducted. All patients received liver biopsy, transient elastography (TE), and 18F-FAPI PET/CT. On 18F-FAPI PET/CT scans, the liver parenchyma's maximum standardized uptake values (SUVmax) were noted. The receiver operating characteristic (ROC) curve analysis was applied to determine the diagnostic efficacy of 18F-FAPI PET/CT in early-stage liver fibrosis (S1 ~ S2) compared with the diagnostic performance of TE.
Results
Of 29 patients, 15(51.7%) had fibrosis S0, 10(34.5%) had S1, and 4(13.8%) had S2 respectively. The SUVmax of patients with early-stage liver fibrosis was significantly higher than those without liver fibrosis in LT recipients and CHB patients (p = 0.004, p = 0.02). In LT recipients, a SUVmax cut-off value of 2.0 detected early-stage liver fibrosis with an AUROC of 0.92 (P = 0.006), and a Liver Stiffness measurements (LSM) score cut-off value of 8.2 kPa diagnosed early-stage liver fibrosis with an AUROC of 0.80 (P = 0.012). In CHB patients, a SUVmax cut-off value of 2.7 detected early-stage liver fibrosis with an AUROC of 0.94 (P < 0.001) and an LSM scores cut-off value of 8.4 kPa diagnosed early-stage liver fibrosis with an AUROC of 0.91 (P < 0.001).
Conclusions
As a non-invasive method, 18F-FAPI PET/CT could be applied to evaluate early-stage liver fibrosis in LT recipients and CHB patients properly. 18F-FAPI PET/CT provided a diagnostic accuracy higher than TE for staging of early-stage liver fibrosis with the additional advantages in whole-liver evaluation.
Deep Learning-Based Diagnosis of Disease Activity in Patients with Graves’ Orbitopathy Using Orbital SPECT/CT
Authors: Yao Ni , Li Longxi , Gao Zhengyuan , Zhao Chen , Li Yanting , Han Chuang , Nan Jiaofen , Zhu Zelin , Xiao Yi , Zhu Fubao , Zhao Min , Zhou Weihua 
Published: October 2022

Abstract
Purpose
Orbital 99mTc-DTPA SPECT/CT is an important new method for the assessment of inflammatory activity in patients with Graves' Orbitopathy (GO), but it consumes a heavy workload for physicians for interpretation. We aim to propose an automated method, called GO-Net, to detect the activity of GO to assist physicians for diagnosis.
Materials and methods
GO-Net had two stages: a semantic V-Net segmentation network (SV-Net) to extract extraocular muscles (EOMs) on orbital CT images; a three-channel convolutional neural network (CNN), including SPECT/CT images and segmentation results, to classify inflammatory activity. Manual corrections were applied when the segmentation results were not accurate. A total of 956 eyes from 478 patients with GO (active: 475; inactive: 481) from Xiangya Hospital of Central South University were enrolled. For the segmentation, five-fold cross-validation with 194 eyes were used for training and internal validation. For the classification, 80% of eyes were trained and internally validated by five-fold cross-validation, and 20% of eyes were used for testing. The contours of the EOMs were drawn manually by an experienced physicians and used as the ground truth. The criteria for the diagnosis of GO activity were determined by the physician through the clinical activity score(CAS) and 99mTc-DTPA uptake.
Results
Our GO-Net method achieved an accuracy of 84.25%, a precision of 83.35%, a sensitivity of 84.63%, a specificity of 83.87%, an F1 score of 0.83, and an area under the receiver (AUC) of 0.89. For EOMs segmentation, our segmentation model achieved a mean intersection over union (IOU) of 0.82. Contours of EOMs in 47 eyes (4.91%) were manually corrected and the average correction time was 5 mins for each eye.
Conclusion
Our proposed Go-Net model could accurately detect GO activity, which has great potential for the diagnosis of GO.
A Historical Comparison of U.S. Army &amp; U.S. Civilian Suicide Rates, 1900-2020
Authors: Smith Jeffrey Allen , Doidge Michael , Hanoa Ryan , Frueh B. Christopher 
Published: October 2022

Abstract
IMPORTANCE: Studies examining the perceived association of war time and increasing suicide rates in the U.S. military and U.S. civilian populations have proliferated since the beginning of the Global War on Terror (GWOT). However, additional historical analysis is needed to better place the recent surge in active-duty U.S. Army and U.S. civilian suicide rates into context and better inform researchers, healthcare providers, and policy makers.
Objective: Compare U.S. Army and U.S. civilian annual suicide data from 1900 to 2020 to identify longitudinal trends and separate them from more acute and transient factors.
DESIGN: Cross sectional study that extracted empirical data from U.S. government websites, publications, and journal articles published from 1900 to 2022.
SETTING: A population-based study of active-duty U.S. Army servicemembers and U.S. civilians from 1900 to 2020.
PARTICIPANTS: All active-duty U.S. Army servicemembers from 1900 to 2020 as detailed in U.S. government publications and journal articles. All U.S. civilians from 1900 to 2020 as detailed in the U.S. Centers for Disease Control and Prevention’s National Vital Statistics System (NVSS).
INTERVENTION(S) or Exposure(S): None.
MAIN OUTCOME(S) AND MEASURE(S): The current study only included data explicitly reported as suicide in the data sources. Suicide rates are per 100,000 individuals.
RESULTS: From 1900 to 2020, active-duty U.S. Army servicemember and U.S. civilian suicide rates appear to fluctuate similarly, but with servicemember rates often displaying more dramatic changes. Since 1900, active-duty U.S. Army servicemember and similarly aged U.S. civilian male suicide rates have gradually converged, with the differences in rates narrowing over time. However, U.S. Army rates appear to break from the U.S. civilian paralleling trend from about 1975 to roughly 1990, when U.S. Army rates decreased and stabilized below the U.S. civilian male rate.
CONCLUSIONS AND RELEVANCE: War does not historically appear to increase suicide rates in active-duty U.S. Army servicemembers or U.S. civilians. More recently, given the apparent convergence of U.S. Army and similarly aged U.S. civilian male annual suicide rates, larger more universal factors than combat may be similarly affecting both populations.
Clinical Investigation of Lymphocyte DNA Damage in COVID-19 Patients
Authors: Doğan Hasan , Kara Aslı , Çankaya Erdem , Balkan Eda , Gürbüz Muhammet Ali , Kızılkaya Murat , Anapalı Merve 
Published: October 2022

Abstract
Background: This prospective cross-sectional study, aimed to evaluate lymphocyte DNA damage in COVID-19 patients. In this prospective cross-sectional prospective cohort study, 50 COVID-19- positive patients attending Erzurum City Hospital Internal Medicine Outpatient Clinic and 42 control group patients were included.
Methods: DNA damages were detected in living cells by lymphocyte isolation in 50 COVID-19- positive patients using the comet assay method. DNA tail/head (olive) moments were evaluated and compared. White blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), neutrophil (NEU), lymphocyte (LYM), eosinophil (EO), monocyte (MONO), basophil (BASO), platelet (PLT), neutrophil/lymphocyte ratio (NLR).
Results: The RBC, lymphocyte, eosinophil, and monocyte means were significantly higher in the control group (p < 0.05). Whereas HGB and neutrophile means were significantly higher in the study group (p < 0.05). There were significant negative correlations between COVID-19 and RBC (r = -0.863), LYM (r = -0.542), EO (r = -0.686), and MONO (r = -0.385). Meanwhile, there were significant positive correlations between COVID-19 and HGB (r = 0.863), NEU (r = 0.307), tail moment (r = 0.598), and olive moment (r = 0.582).
Conclusion: Both the tail and olive moment mean differences were significantly higher in the study group with higher ranges (p < 0.05). COVID-19 infection statistically significant is increasing both the tail and olive damage percentage in patients, causing DNA damage. Lastly, the NLR rate was associated with the presence and progression of COVID-19.
Crossing the Inflection Point of Digital Development "Emission Reduction" - An Analysis Based on the Perspective of Technological Innovation
Authors: Li Haihai , Huang Yanshuo , Liao Qian 
Published: October 2022

Abstract

From the context of China's "peak carbon dioxide emissions" and "carbon neutral" goals, this study determines whether digital development, as an important path of China's modern development, contributes to the achievement of China's "carbon neutral" goals. In addition, this paper examines whether digital development as an important path of China's modern development also contributes to realizing China's "carbon neutral" goal, while taking into account the internal logic and theoretical mechanism. Consequently, panel data of 267 prefecture-level cities and above nationwide for the time period ranging from 2003 to 2017 is constructed in this study, and used the two-way fixed effects model and instrumental variable estimation method to verify the intrinsic mechanism of digital development affecting carbon intensity. The study findings indicate that: (1) digital development exerts a significant inhibitory effect on carbon intensity, and overall, it demonstrates an "inverted U-shaped" relationship of promotion in the first place and inhibition in the subsequent phase. Moreover, the proposed inhibitory effect represents the marginally increasing trend; (2) promoting technological innovation serves as a significant mechanism for digital development to inhibit the carbon intensity; (3) most cities in China are still below the inflection point of digitalization; (4) optimizing the regional innovation environment effectively advances the inflection point of digitalization; thereby, implying that the government should pay due attention to the inhibiting effect of digitalization on carbon intensity. Besides, there is also a need to further optimize the environment of regional innovation, increase innovation investment, and smoothen the influence mechanism of technological innovation in order to quickly cross the "emission increase" period of digitalization development.
Long COVID and Associated Factors in Individuals From Southern Brazil: A Population-based Study on COVID
Authors: Saes Mirelle de Oliveira , Vieira Yohana Pereira , Rocha Juliana Quadros Santos , Silva Carine Nascimento , Soares Abelardo Oliveira , Neves Rosália Garcia , Gonçalves Cristiane de Souza , Duro Suele Manjourany Silva 
Published: October 2022

Abstract

COVID-19 is an infection in which symptoms may persist after the acute phase. The aim of the present study was to verify the prevalence and factors associated with COVID-19 and to describe the main residual symptoms after 6–10 months of infection in individuals from the extreme south of Brazil. This was a cross-sectional study of 2919 individuals older than 18 diagnosed by RT‒PCR with COVID-19 in the city of Rio Grande/RS, Brazil, from December 2020 to March 2021 who were symptomatic, with data collected by telephone interview or home visit. The COVID-19 outcome was obtained from the sum of all symptoms that the individual claimed to continue having at the time of the interview, ranging from zero to 19. For the crude and adjusted analyses, we used Poisson regression with robust adjustment of variance calculating prevalence ratios (PRs) and their respective 95% confidence intervals. The adjusted analysis was performed by means of a hierarchical model built in four levels. The significance level adopted was 5%, and the analyses were performed with the statistical package Stata 16.1. The prevalence of COVID-long was 48.3% (95% CI 46.5; 50.1). The most prevalent residual symptoms were fatigue, memory loss, loss of attention, headache, loss of smell, muscle pain and loss of taste. The groups more likely to develop COVID-19 were female, exhibited anxiety, hypertension, heart problems, diabetes mellitus, musculoskeletal problems, respiratory problems, previous morbidities and hospitalization in a ward or ICU. Physical activity and self-perception of good and very good health were protective factors for the outcome. When adjusted, female gender, anxiety, morbidities and who were hospitalized remained associated with the outcome, while self-perception of good and very good health had a lower probability. This study showed that almost half of the individuals in the sample developed long COVID after 6–9 months of infection. Among the associated factors, female individuals and those with chronic conditions stand out, demanding the creation of public policies to promote integral and continued assistance to these individuals.