bluuebunny/crossref_metadata_2025_split huggingface dataset:
Channel estimation based on superimposed pilot and weighted averaging
Authors: Wu Qun , Zhou Xiao , Wang Chengyou , Qin Zhiliang 
Published: June 2022

AbstractChannel estimation based on superimposed pilot (SP) is a challenge in orthogonal frequency division multiplexing (OFDM) systems. To reduce the pilot data interference in the SP and estimate the channel state information accurately, a weighted averaging (WA) channel estimation method based on the superimposed pilot is proposed in this paper. At the transmitter, two signals with data symbols and pilot symbols superimposed at different subcarriers are transmitted. At the receiver, the elimination scheme is proposed to remove the pilot data interference. Based on the temporal correlation of wireless channels, the WA method is used to reduce the interference caused by additive white Gaussian noise in the channel. Simulation results verify that the proposed method can be applied to different channel scenarios. It has better normalized mean square error and bit error rate performance than other existing methods, and the superimposed pilot can improve the throughput of wireless communication systems.
Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades
Authors: Campioni Fábio , Vilela Felipe Pinheiro , Cao Guojie , Kastanis George , dos Prazeres Rodrigues Dália , Costa Renata Garcia , Tiba-Casas Monique Ribeiro , Yin Lanlan , Allard Marc , Falcão Juliana Pfrimer 
Published: June 2022

AbstractSalmonella Dublin is a cattle-associated serovar sporadically causing disease in humans. S. Dublin strains isolated in Brazil and in other countries were analyzed to determine their phylogenetic relationships, the presence of genes, plasmids, genomic regions related to virulence and antimicrobial resistance genes repertoire, using WGS analyses. Illumina was used to sequence the genome of 112 S. Dublin strains isolated in Brazil from humans (n = 82) and animals (n = 30) between 1983 and 2016. Furthermore, 87 strains from other countries were analyzed. WGSNP analysis revealed three different clades, in which the strains from Brazil belonged to two clades, A and C. Most of the genes and genomic regions searched varied among the strains studied. The siderophore genes iroB and iroC were exclusively found in strains from Brazil and pegD gene, related to fimbrial adherence determinants, were positive in 124 strains from clades A and B but absent in all the strains from clade C (n = 71). Eleven plasmid replicons were found in the strains from Brazil, and nine were exclusively found in strains from other countries. The antimicrobial resistance genes mdsA and mdsB, that encode an efflux pump, were found in all the strains studied. The strains from Brazil carried other resistance genes, such as tet(A) (n = 11), tet(B) (n = 4) and tet(C) (n = 4), blaTEM-1 (n = 4), catA1 (n = 1), aadA1 (n = 1), and sul1 (n = 1). In conclusion, S. Dublin strains isolated in Brazil presented some few unique genes not found in strains from other countries and were allocated into two distinct clades with strains of human and animal origin epidemiologically related. This fact stresses the zoonotic potential of S. Dublin circulating in Brazil for more than 30 years.
A data model and file format to represent and store high frequency energy monitoring and disaggregation datasets
Authors: Pereira Lucas , Velosa Nuno , Pereira Manuel 
Published: June 2022

AbstractThere is a generalized consensus in the Non-Intrusive Load Monitoring research community on the importance of public datasets for improving this research field. Still, despite the considerable efforts to release public data, what is currently available suffers from serious issues, among which is the lack of widely accepted data models and common interfaces to access the currently available and future datasets. This paper proposes the Energy Monitoring and Disaggregation Data Format (EMD-DF64). EMD-DF64 is a data model, file format, and application programming interface developed to provide a unique interface to create, manage, and access high-frequency (≥ 1 Hz) electric energy consumption datasets. More precisely, the present paper describes the data model and its respective implementation, which was done by leveraging the well-known Sony WAVE64 format that supports the storage of audio data and metadata annotations.
RNA modification patterns based on major RNA modifications define tumor microenvironment characteristics in glioblastoma
Authors: Li Ganglei , Zhu Yu , Gu Jun , Zhang Tiesong , Wang Feng , Huang Kaiyuan , Gu Chenjie , Xu Kangli , Zhan Renya , Shen Jian 
Published: June 2022

AbstractRNA modifications play a major role in tumorigenicity and progression, but the expression and function in glioblastoma (GBM) have not been well described. In this study, we developed a GBM score based on the differentially expressed genes (DEGs) between groups showing RNA modification patterns. We assessed the association between the GBM score and tumor microenvironment (TME) characteristics. Based on the gene expression of these regulators, we identified two clusters with distinct RNA modification patterns. Kaplan–Meier survival curves showed that patients in cluster 1 had worse survival than those in cluster 2. Kaplan–Meier and multivariate Cox regression analyses showed that GBM scores (based on DEGs between RNA modification patterns) are an independent predictive biomarker for patient prognosis. Besides, we found that samples with high scores were significantly associated with epithelial-to-mesenchymal transition and immune checkpoints, while samples with low scores were associated with cell cycle regulation. Importantly, GBM-score markedly positively correlated drug resistance, while negatively correlated with drug sensitive. The responders of anti-PD-1/PD-L1 immunotherapy tend to have a lower GBM score than non-responders. In conclusion, our comprehensive analysis of multiple RNA modifications in GBM revealed that RNA modification regulators were closely correlated with TME.
Biofilm accumulation and sucrose rinse modulate calcium and fluoride bioavailability in the saliva of children with early childhood caries
Authors: Crescente Camila Lopes , de Sousa Emerson Tavares , Lima-Holanda Aline Tavares , Steiner-Oliveira Carolina , Nobre-dos-Santos Marinês 
Published: June 2022

AbstractThis study aimed at investigating the combined effect of biofilm accumulation and 20% sucrose rinse on the modulation of calcium (Ca2+), phosphate (Pi), and fluoride (F−) bioavailability in the saliva of children with early childhood caries (ECC). Fifty-six preschoolers of both genders were evaluated according to caries experience and activity: caries-free (CF, n = 28) and with ECC (n = 28) and then, submitted to biofilm intervention (biofilm accumulation). In each situation, saliva samples were collected before and five minutes after a 20% sucrose rinse to determine the concentrations of Ca2+, Pi, and F−. Calcium concentration was significantly lower in the biofilm accumulation situation compared to the situation of biofilm mechanical control (p ≤ 0.01), except for CF children after sucrose rinse. Biofilm accumulation increased salivary calcium concentration in children with ECC after sucrose rinse (p = 0.04), whereas mechanical biofilm control reduced it in both groups (p = 0.000). Phosphate concentration was influenced by mechanical control of biofilm in CF children (p = 0.03). The fluoride bioavailability was reduced by sucrose rinse and biofilm accumulation in CF and ECC children (p ≤ 0.002). In conclusion, the combined effect of biofilm accumulation and sucrose rinse modifies the bioavailability of calcium and fluoride in the saliva of children with early childhood caries.
Efficacy and safety of radiofrequency ablation versus parathyroidectomy for secondary hyperparathyroidism in dialysis patients: a single-center retrospective study
Authors: Ren Mian , Zheng Danna , Wu Juan , Liu Yueming , Peng Chengzhong , Shen Wei , Lin Bo 
Published: June 2022

AbstractWe compared the efficacy and safety of ultrasound (US)-guided radiofrequency ablation (RFA) and parathyroidectomy (PTX) for the treatment of secondary hyperparathyroidism (SHPT). In this single-center retrospective study, we divided patients into PTX (n = 53) and RFA (n = 47) groups. The primary outcome was the proportion of patients who achieved the target intact parathyroid hormone (iPTH) concentration range (≤ 300 pg/mL). Secondary outcomes were the differences in the changes in iPTH, calcium, and phosphorus levels over time and prognosis. iPTH concentrations of 82.1% and 64.1% in the PTX and RFA groups, respectively, were within the recommended range at the endpoint (P = 0.07). iPTH concentrations in the PTX and RFA groups dropped sharply after treatment (82 ± 163 pg/mL and 280 ± 307 pg/mL, respectively, P < 0.001). There was no difference in the trends of iPTH, calcium, and phosphorus levels between the two groups (P > 0.05). Survival analysis revealed no differences in all-cause mortality and cumulative response rate between the two groups (P = 0.90, P = 0.14, respectively). Notably, the incidence of infection and length of the hospital stay in the RFA group were significantly lower. The preoperative bone-specific alkaline phosphatase concentration was a risk factor for postoperative hypocalcemia. US-guided RFA is minimally invasive and compared to PTX in terms of long-term efficacy and complications in the treatment of severe SHPT in maintenance dialysis patients. It may be used as an alternative technique to PTX; however, further studies are needed.
Expression, characterization, and activity optimization of a novel cellulase from the thermophilic bacteria Cohnella sp. A01
Authors: Mohammadi Shima , Tarrahimofrad Hossein , Arjmand Sareh , Zamani Javad , Haghbeen Kamahldin , Aminzadeh Saeed 
Published: June 2022

AbstractCellulases are hydrolytic enzymes with wide scientific and industrial applications. We described a novel cellulase, CelC307, from the thermophilic indigenous Cohnella sp. A01. The 3-D structure of the CelC307 was predicted by comparative modeling. Docking of CelC307 with specific inhibitors and molecular dynamic (MD) simulation revealed that these ligands bound in a non-competitive manner. The CelC307 protein was purified and characterized after recombinant expression in Escherichia coli (E. coli) BL21. Using CMC 1% as the substrate, the thermodynamic values were determined as Km 0.46 mM, kcat 104.30 × 10–3 (S−1), and kcat/Km 226.73 (M−1 S−1). The CelC307 was optimally active at 40 °C and pH 7.0. The culture condition was optimized for improved CelC307 expression using Plackett–Burman and Box–Behnken design as follows: temperature 20 °C, pH 7.5, and inoculation concentration with an OD600 = 1. The endoglucanase activity was positively modulated in the presence of Na+, Li+, Ca2+, 2-mercaptoethanol (2-ME), and glycerol. The thermodynamic parameters calculated for CelC307 confirmed its inherent thermostability. The characterized CelC307 may be a suitable candidate for various biotechnological applications.
Inverted multi-layer internal limiting membrane flap for macular hole retinal detachment in high myopia
Authors: Wang Xianggui , Zhu Ying , Xu Huizhuo 
Published: June 2022

AbstractTo investigate the surgical outcomes of pars plana vitrectomy (PPV) combined with inverted multi-layer internal limiting membrane (ILM) flap for the treatment of macular hole retinal detachment in high myopia. We retrospectively analysed the medical records of macular hole retinal detachment (MHRD) patients with high myopia. The patients were divided into two groups with different surgical procedure: inverted multi-layer ILM flap group (group 1, 27 eyes) and the ILM peeling group (group 2, 29 eyes). Retinal reattachment rate, macular hole closure rate at last follow-up and BCVA at 6 months post-operation were compared between the two groups. After primary PPV and silicone oil removal, the retinal reattachment rate was 96.3% in group 1 and 93.1% in group 2 respectively at last follow-up, showing no statistically significant difference (odds ratio = 0.525, P = 1.000). All eyes in group 1 had type I macular closure (100%, 27/27), while only 7 eyes (24.1%, 7/29) in group 2 have type I macular hole closure. The difference was statistically significant (odds ratio = 0, P < 0.05). The mean logMAR BCVA both improved significantly at 6 months post-operation compared with pre-operation (t = 4.181, P < 0.001; t = 3.217, P < 0.001), however the difference of post-operation BCVA between the two groups was not statistically significant (t = 0.906, P > 0.05). PPV combined with inverted multi-layer ILM flap could achieve better anatomical outcomes than ILM peeling technique with no significant advantage in functional outcomes.
Protocatechuic acid as a potent anticarcinogenic compound in purple rice bran against diethylnitrosamine-initiated rat hepatocarcinogenesis
Authors: Punvittayagul Charatda , Luangsuphabool Theerapat , Wongpoomchai Rawiwan 
Published: June 2022

AbstractOur previous study demonstrated that purple rice bran extract (PRBE) could inhibit diethylnitrosamine (DEN)-induced hepatocarcinogenesis. Protocatechuic acid (PCA) is the major phenolic acid contained in the PRBE. Therefore, this study aimed to determine whether PCA is an anticarcinogenic compound in purple rice extract. Rats were intraperitoneally injected with DEN to induce glutathione S-transferase placental form (GST-P)-positive foci. Rats were fed with PRBE at 500 mg kg−1 body weight or PCA at 4 mg kg−1 body weight for 5 and 15 weeks. PCA administration attenuated DEN-induced hepatic GST-P positive foci to a degree similar to PRBE. The molecular mechanisms of PCA in the initiation stage were correlated with reduced activity of cytochrome P450 reductase and induction of glutathione S-transferase. In addition, PCA also downregulated the expression of TNF-α and IL-1β genes in rat liver. These genes are associated with the inhibition of inflammation. In the promotion stage, PCA suppressed cell proliferation correlated with the downregulation of Cyclin D1 expression. Moreover, it also induced apoptosis, indicated by increased expression of P53 and Bad genes, and decreased the expression of the anti-apoptotic Bcl-xl in DEN-initiated rats. These findings suggest that PCA is an active compound in the anticarcinogenic action of purple rice bran.
Poly(A) capture full length cDNA sequencing improves the accuracy and detection ability of transcript quantification and alternative splicing events
Authors: Ura Hiroki , Togi Sumihito , Niida Yo 
Published: June 2022

AbstractThe full-length double-strand cDNA sequencing, one of the RNA-Seq methods, is a powerful method used to investigate the transcriptome status of a gene of interest, such as its transcription level and alternative splicing variants. Furthermore, full-length double-strand cDNA sequencing has the advantage that it can create a library from a small amount of sample and the library can be applied to long-read sequencers in addition to short-read sequencers. Nevertheless, one of our previous studies indicated that the full-length double-strand cDNA sequencing yields non-specific genomic DNA amplification, affecting transcriptome analysis, such as transcript quantification and alternative splicing analysis. In this study, it was confirmed that it is possible to produce the RNA-Seq library from only genomic DNA and that the full-length double-strand cDNA sequencing of genomic DNA yielded non-specific genomic DNA amplification. To avoid non-specific genomic DNA amplification, two methods were examined, which are the DNase I-treated full-length double-strand cDNA sequencing and poly(A) capture full-length double-strand cDNA sequencing. Contrary to expectations, the non-specific genomic DNA amplification was increased and the number of the detected expressing genes was reduced in DNase I-treated full-length double-strand cDNA sequencing. On the other hand, in the poly(A) capture full-length double-strand cDNA sequencing, the non-specific genomic DNA amplification was significantly reduced, accordingly the accuracy and the number of detected expressing genes and splicing events were increased. The expression pattern and percentage spliced in index of splicing events were highly correlated. Our results indicate that the poly(A) capture full-length double-strand cDNA sequencing improves transcript quantification accuracy and the detection ability of alternative splicing events. It is also expected to contribute to the determination of the significance of DNA variants to splicing events.
Preventive control of successive failures in extreme weather for power system resilience enhancement
Authors: Zhang Zhenyu , Cui Wei , Niu Shuanbao , Wang Cong , Xue Chen , Ma Xiaowei , Liu Shiyu , Bie Zhaohong 
Published: June 2022

AbstractSuccessive failure propagating through a power system, typically caused by extreme weather such as storm and lightning, can cause blackouts by means of a variety of processes. To address this issue, this paper proposes preventive control for successive failures of power system to mitigate the consequences of successive failures in extreme weather. First, based on the Poisson process theory, the authors model the successive failure propagation, and present the confidence interval estimation for the time interval between successive failures. Then, a failure pre‐control methodology that quantifies the grid regulating ability and actions is developed to make decisions for system operators on mitigating overloads of transmission lines. Furthermore, a systematic pre‐control framework for successive failures is first proposed to detect latent risks, eliminate predictable consequential outages, and secure power system stability. Results from IEEE‐9 bus and IEEE‐39 bus standard systems as well as a real‐world system show the feasibility and effectiveness of the proposed method. The proposed preventive control framework can provide a systematic pre‐control action scheme for system operators, and realize enhancement of stability and resilience to power system failures.
Analysis of light and no‐load operation of a 300 kW resonant single active bridge based on 3.3 kV SiC‐devices
Authors: Fortes G. , Ladoux P. , Fabre J. , Flumian D. 
Published: June 2022

AbstractThe resonant single active bridge topology (R‐SAB) operated in the half‐cycle discontinuous current mode (HC‐DCM) is a very attractive solution due to its high efficiency, low complexity and fixed voltage transfer ratio (DCX). However, as expected for a series‐resonant converter (SRC), its DCX operation depends on the resonant tank circuit‐parameters, parasitic capacitive elements and output load. Specially, at light and no‐load operation, when the system is extremely underdamped, it may present a large output overvoltage due to resonance interactions. This is of prime importance for converters using medium voltage (MV) SiC‐MOSFETs, which feature significant output capacitances that can lead to voltage breakdown of the rectifier semiconductors. Therefore, the supposed fixed‐voltage transfer ratio is not entirely valid and deserves a proper understanding due its criticality. This paper reviews the subject, clarifying its root cause and its multifactorial dependencies. Moreover, it provides a simple solution based on a variable dead‐time with fixed magnetizing current experimentally verified with a 300kW/1.8kV R‐SAB prototype implemented with MV SiC‐devices.
Design method of wide‐band high‐current air‐core inductor EMI filter for high voltage DC power of motor inverter of electric vehicle
Authors: Zhai Li , Yang Shuangjie , Hu Guixing , Lv Mengyuan 
Published: June 2022

AbstractPassive filters are often used for electromagnetic interference (EMI) suppression of high voltage (HV) DC power supplies of electric vehicle (EV) motor controllers. The high current of the DC bus saturates the differential mode (DM) magnetic ring, while the leakage inductance of common mode (CM) inductor used as the DM inductance is usually very small, resulting in almost no suppression of DM EMI. A design method of broadband high‐current air‐core inductor passive filter is proposed. By using the air‐core inductor as DM inductor, the problem of easy saturation of the DM inductor core and the small leakage inductance of the CM inductor is solved, and improves the low frequency DM EMI suppression. Through the calculation and selection of the turning frequency point of the multi‐stage filter circuit, the effective frequency band can be improved. By analysing the influence of parasitic parameters, the problem of insufficient insertion loss of the filter at high frequency is solved. The experimental verification shows that the proposed air‐core inductor filter achieves 50 dB of CM and DM insertion loss in frequency range of 150 kHz–108 MHz, and meets the requirements of system conducted disturbance voltage specified in CISPR25‐2016Rd level 3.
Scutellaria baicalensis Pith-decayed Root Inhibits Macrophage-related Inflammation Through the NF-κB/NLRP3 Pathway to Alleviate LPS-induced Acute Lung
Injury
Authors: Zhang Fanglei , Ke Chang , Zhou Zhongshi , Xu Kang , Wang Yan , Liu Yanju , Tu Jiyuan 
Published: June 2022

AbstractAcute lung injury (ALI) is one of the representative “lung heat syndromes” in traditional Chinese medicine (TCM). Scutellaria baicalensis is an herbal medicine used in TCM for
treating lung diseases, due to its remarkable anti-inflammatory and antiviral effects. When used in TCM, S. baicalensis root is divided into two categories: S. baicalensis
pith-not-decayed root (SN) and S. baicalensis pith-decayed root (SD). Compared to SN, SD has a better effect on lung diseases. We constructed a lipopolysaccharide (LPS)-induced acute
lung injury (ALI) mouse model to study the pharmacodynamic mechanism of SD. The ethanolic extract of Scutellaria baicalensis pith-decayed root (EESD) significantly affected
LPS-induced ALI by reducing alveolar interstitial thickening, pulmonary edema, and other pathological symptoms, decreasing the infiltration of inflammatory cells, especially macrophages, and
inhibiting IL-1β, TNF-α, and IL-6 transcription and translation. Furthermore, in the THP-1 macrophage model induced by LPS, EESD inhibited the expression of phosphorylated
nuclear factor inhibitory protein alpha (p-IκBα), phosphorylated nuclear factor-κB P65 (p-p65), cleaved-caspase-1, cleaved-IL-1β protein, and the release of
inflammatory factors in the NF-κB/NLRP3 pathway, inhibiting macrophage function. In vivo experiments yielded similar results. Therefore, the present study clarified the
potential of EESD in the treatment of ALI and revealed its potential pharmacodynamic mechanism by inhibiting the NF-κB/NLRP3 inflammasome pathway and suppressing the pro-inflammatory
phenotype activation of lung tissue macrophages.