bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ocular Changes in Children With Familial Mediterranean Fever: The Effect of Subclinical Inflammation?
Authors: Sav Nadide Melike , Teberik Kuddusi 
Published: September 2022

Abstract
Purpose: Elevation of acute phase reactants during attack is an important indicator of acute inflammation in Familial Mediterranean Fever (FMF). However, the disease course also involves inflammation in remission period. Subclinical inflammation is a major etiological factor for organ involvement of the disease. The eye is one of the target organs in the course of FMF.Methods: The study included 51 patients with FMF in remission period for at least 3 months and 51 age-matched healthy individuals. Intraocular pressure, axial length, peripapillary retinal nerve fiber layer (RNFL) thickness, central macular thickness, and subfoveal choroidal thickness were assessed for patient and control groups using spectral domain optical coherence tomography (SD-OCT). Patients were grouped according to disease severity scores, and laboratory and ocular findings were compared.Results: Serum amyloid A, C-reactive protein and fibrinogen were higher and temporal inferior RNFL was thinner in the patient group than in the control group (p<0.05). Central macular, submacular choroidal, nasal and temporal thicknesses were lesser in the patient group than in the control group, but there was no statistically significant difference between the groups (p>0.05). On the other hand, a positive correlation was found between proteinuria and axial length in the patient group. Conclusion: Subclinical inflammation is one of the factors responsible for important changes in the eye in FMF. Some changes that occur in the early period can become more prominent with a longer duration of the disease. Follow-up from childhood to adulthood would enable revealing the effects of subclinical inflammation in these patients.
Polystyrene nanoplastic exposure enhances LPS-induced lung inflammation in mice by inducing M1 polarization of macrophages via ROS/JAK/STAT signaling pathway
Authors: Fan Xue , Xu Tong , Peng Lin , Tang Xinyu , Chi Qianru , Li Zhe , Shi Guangliang , Li Shu 
Published: September 2022

Abstract
Nanoplastics (NPs) are an emerging environmental pollutant. NPs can spike various tissues results to oxidative stress and tissue damage in organisms. While recent studies have reported a relationship between nanoparticles and respiratory system injury, the specific mechanism of NP exposure-induced lung damage remains to be explored. In the present study, C57BL6 male mice were treated intraperitoneal injection of PS-NPs and/or LPS. The relevant indicators were detected by HE staining, western blotting and qRT-PCR. RAW264.7 was pretreated with JAK2 inhibitor (AG490) to verify whether the JAK2/STAT3 signaling pathway is involved in PS-NPs exposure enhances LPS-induced pulmonary inflammatory response. We found decreased antioxidant capacity in mice lungs, activation of the JAK2/STAT3 pathway, and the expression levels of macrophage M1 marker genes increased (including CD16, CD86, and MCP1), while macrophage M2 marker genes (including CD206, PPARγ, and Arg1) expression levels decreased, resulting in a macrophage M1/M2 imbalance. In addition, PS-NPs can increase the expression of inflammation-related factors IL-1β, TNF-α and IL-6. In in vitro experiments, we obtained similar results to in vivo experiments. More importantly, the JAK2 pathway inhibitor AG490 reversed ROS-induced changes in macrophage imbalance and inflammation in PS-NPs and LPS-exposed RAW264.7 cells. In conclusion, PS-NPs activated the ROS/JAK/STAT pathway, aggravated LPS-induced lung M1/M2 macrophage imbalance and promoted inflammatory responses. Our results enrich the toxic effects and related molecular mechanisms of NPs-induced lung inflammation, and provide new insights into the toxic effects of NPs on mammals.
An evaluation of seasonal and spatial variation of water quality parameters of the Mamloo Reservoir in Iran by multivariable analysis
Authors: Naderi H. , Javid A. H. , Borgheic Seyed M. , Eslamizadehd M. 
Published: September 2022

Abstract
Lakes and reservoirs have a remarkable role in specifying the ecological environment, public health and socio-economic development. The devastating impacts, of human activities, related to the water quality of these resources, have remarkably elevated in the recent decades. The objectives of this study, is to evaluate the water quality of the Mamloo Dam reservoir (MDR), comprehensively, by analyzing seasonal and spatial modifications of the 10 focal water quality parameters at the four sampling points in 2019. An analysis of the spatial behavior of the parameters was executed in the reservoir the by using the Inverse Distance Weighting Interpolation Approach. The Pearson correlation was carried out, to explore the correlation and attain a better description, in relative to the behavior of the entire parameters of water quality. Ten of the principal components (PC) were obtained, which described 99 percent of the total data changes approximately. The Carlson's Trophic State Index (TSI), demonstrated the presence of eutrophic conditions in the entire sampling stations of the MDR. A survey of the spatial behavior of water quality parameters validates the impact of human and natural activities in the watershed basin, in relevance with the quality of water. Ultimately, the results which have come to hand portrayed that, if in the case, there is an absence of an appropriate and desirable management, a threat of an exacerbation of contamination in the water body, as well as a dramatic degradation of the ecosystem in the reservoir, could be extremely probable.
Ingestion of rubber tips of artificial turf field by goldfish
Authors: Chiba Rihito , Fujinuma Ryosuke , Yoshitomi Tomoyasu , Shimizu Yasuo , Kobayashi Makito 
Published: September 2022

Abstract
Marine microplastic is one of the global environmental issues. The present study examined whether rubber tips of artificial sports fields could be marine microplastic. We observed the migration of rubber tips from the artificial turf field to the surrounding ditch connected to sewer pipes and then examined the ingestion of rubber tips using goldfish Carassius auratus. The rubber tips found in sediments in the ditch suggest that the rubber tips could be sent to the river and released into the ocean. The goldfish ingested rubber tips with or without fish feed, and rubber tips are found in the intestine. However, the fish discharged the rubber tips within 48 h after the ingestion. These results indicate that ingestion of the rubber tips was not accidental but active behavior. Therefore, the artificial turf sports fields could be a source of marine microplastic and may cause hazardous effects on wild fishes through ingestion.
Fast Reconstruction Method of Three-dimension Model Based on Dual RGB-D Cameras for Peanut Plant
Authors: Liu Yadong , Yuan Hongbo , Zhao Xin , Fan Caihu , Cheng Man 
Published: September 2022

Abstract

Plant shape and structure are important factors in peanut breeding research. Constructing a three-dimension (3D) model can provide an effective digital tool for comprehensive and quantitative analysis of peanut plant structure. A 3D reconstruction method based on dual RGB-D cameras was proposed for the peanut plant 3D model quickly and accurately. The two Kinect v2 were mirror symmetry placed on both sides of the peanut plant, and the point cloud data obtained were filtered twice to remove noise interference. After rotation and translation based on the corresponding geometric relationship, the point cloud acquired by the two Kinect v2 was converted to the same coordinate system and spliced into the 3D structure of the peanut plant. The experiment was conducted at various growth stages based on twenty potted peanuts. The plant traits’ height, width, length, and volume were calculated through the reconstructed 3D models, and manual measurement was carried out at the same time. The accuracy of the 3D model was evaluated through a synthetic coefficient, which was generated by calculating the average accuracy of the four traits. The test result shows that the synthetic accuracy of the reconstructed peanut plant 3D model by this method is 93.42%. A comparative experiment with the iterative closest point (ICP) algorithm, a widely used 3D modeling algorithm, was additionally implemented to test the rapidity of this method. The test result shows that the proposed method is 2.54 times faster with approximated accuracy compared to the ICP method. This approach should be useful for 3D modeling and phenotyping peanut breeding.
A life cycle assessment of drilling waste management in Russia
Authors: Ilinykh Galina , Fellner Johann , Sliusar Natalia , Korotaev Vladimir 
Published: September 2022

Abstract
Oil production is currently impossible without drilling wells, so millions of tons of drilling waste contaminated with oil, chlorides, and heavy metals are generated every year in Russia alone. This article presents the results of a comparative life cycle assessment of water-based drill cuttings management technologies applied in Russia, including disposal, solidification, and reinjection. Life cycle assessment of the drilling waste management was performed using OpenLCA software, Ecoinvent 3.8 database and ReCiPe Midpoint (H) impact assessment method. Data from oil producing companies on the composition of drilling waste and information from drilling waste treatment companies on the technologies and reagents used were also applied. To compare alternative technologies the following scenarios were compared: Scenario 0 «Landspreading», scenario 1 «Disposal», scenario 2 «Solidification» (scenario 2a – in a waste pit, scenario 2b – without a waste pit), and scenario 3 «Reinjection». Sensitivity analysis was performed to test for variations in results for oilfields located in different regions. Results and discussion: The environmental impact of scenario 0 depends mostly on drilling waste composition, which is largely determined by human toxicity. In scenario 1, sand extraction and transportation, excavator and bulldozer operations were the most impactful processes. Among all scenarios, 2a and 2b have the biggest environmental impact in most categories. The production of cement and lime for drilling waste solidification was the main contributor to fossil depletion (64% of the total amount for scenario 2a and 54% for scenario 2b), and greenhouse gas emissions (49% of the total amount for scenario 2a and 70% for scenario 2b). The main contributor in scenario 3 is electricity production. Oilfield location does not affect the data for reinjection, but the impact assessment changes up to 60% for drill cutting disposal due to different waste pit design depending on permafrost and groundwater levels. Drilling waste landspreading is only the best option if the level of pollutants in the waste is very low. Among the other scenarios of drill cuttings management aimed at isolating pollutants from the environment, solidification technologies have the greatest environmental impact, primarily due to their use of binders. However, the application of soil-like material (solidified drill cuttings) as an inert ground in swampy areas can make migration of heavy metals possible. So, reinjection is associated with the least impact on the environment.
Comparison of genetic susceptibility to lung adenocarcinoma and squamous cell carcinoma in Japanese patients using a novel panel for cancer-related drug- metabolizing enzyme genes
Authors: Ohnami Sumiko , Naruoka Akane , Mizuguchi Maki , Nakatani Sou , Kamada Fukumi , Shimoda Yuji , Sakai Ai , Ohshima Keiichi , Hatakeyama Keiichi , Maruyama Kouji , Isaka Mitsuhiro , Ohde Yasuhisa , Kenmotsu Hirotsugu , Takahashi Toshiaki , Akiyama Yasuto , Nagashima Takeshi , Urakami Kenichi , Ohnami Shumpei , Yamaguchi Ken 
Published: September 2022

Abstract
The differences in genetic susceptibility to lung adenocarcinoma and squamous cell carcinoma are unclear. Hence, we developed a customized, targeted gene sequencing panel for efficient and sensitive identification of germline variants, including whole-gene deletion types for cancer-related drug-metabolizing enzyme genes in lung adenocarcinoma and squamous cell carcinoma. The minor allele frequencies of the variants, confirmed as clinically significant in the Japanese population, did not differ significantly from those of normal participants listed in the public database. Genotype analysis comparing lung adenocarcinoma (n = 559) and squamous cell carcinoma (n = 151) indicated that the variants of DPYD (rs190771411, Fisher’s exact test, P = 0.045; rs200562975, P = 0.045) and ALDH2 (rs568781254, P = 0.032) were associated with an increased risk of squamous cell carcinoma compared to adenocarcinoma. Conversely, whole-gene deletion of CYP2A6 was associated with adenocarcinoma but not squamous cell carcinoma. Notably, whole-gene deletion of CYP2A6 was confirmed in 22 patients with lung adenocarcinoma but in no patients with squamous cell carcinoma. The majority of patients with whole-gene deletion of CYP2A6 were female non-smokers. The discovery of a whole-gene deletion of CYP2A6 in patients with lung adenocarcinoma may have an important role in clinical practice and advance our understanding of CYP2A6 germline variants and their association with carcinogenesis or their susceptibility to lung adenocarcinoma.
FAM83B promotes the invasion of primary lung adenocarcinoma via PI3K/AKT/NF-κB pathway
Authors: Zhang Jing , Wang Jiajia , Yue Ke , Li Panpan , Shen Wenping , Qiao Xiaowen , Wang Yan , Wu Xiaojuan 
Published: September 2022

Abstract
Objects: The family with sequence similarity 83B (FAM83B) is
one of the markers for poor prognosis in several carcinomas, but
the expression and the mechanism resulted in malignant phenotype in
lung adenocarcinoma (LUAD) remain to be elucidated.

Methods: Data of RNA-seq in LUAD were downloaded from the
cancer genome atlas (TCGA) database for differential expression and
survival analysis, and immunohistochemistry was employed to analyze
the protein expression of FAM83B in 126 cases of primary LUAD. The
LUAD cell lines were collected for the detection of the effects on
migration and invasion. Then, western blot was performed to measure
the expression of tissue inhibitor of metalloproteinase (TIMP)-1
and activation of PI3K/AKT/ NF-κB pathway.

Results: FAM83B was overexpressed in multiple types of
carcinomas; The differential expression analysis revealed that the
level of FAM83B was higher in LUAD than that in para-carcinoma; The
patients with overexpression of FAM83B were with shorter overall
survival (OS), disease specific survival (DSS) and progress free
interval (PFI); Enrichment analysis suggested it was related to the
focal adhesion of LUAD. Immunohistochemistry analysis demonstrated
that higher FAM83B expression was positively related to lymph node
metastasis in primary. Scratch assay and Borden chamber assay
showed that the overexpression of FAM83B promoted migration and
invasion activity in vitro. Furthermore, high level of FAM83B
accelerated the tumorigenesis in vivo. Western blot showed that
TIMP-1 was upregulated in H1299/FAM83B OE cells accompanying by the
activation of PI3K/AKT/NF-κB pathway.

Conclusions: FAM83B was a marker for poor prognosis of LUAD and
it might promote the expression of TIMP-1 by activating
PI3K/AKT/NF-κB pathway and then affect the ECM balance, which
resulted in the migration and invasion of LUAD.
Association of Air Pollution with Meteorological Factors during COVID-19 pandemic in India: A Systematic Review
Authors: Singh Bhupendra Pratap , Dhalan Sumit Singh , Kumari Sweety , Razi Aasia 
Published: September 2022

Abstract
An unprecedented outbreak of novel coronavirus disease 2019 (COVID-19), leading to the pandemic, has affected almost all countries, adversely affecting human health and the environment. Nearly 2.3 billion people live in areas where air pollution levels are high, particularly in the Asia-Pacific region and more likely in countries such as India. Prolonged exposure to air pollution is known to have adverse effects on humans and the environment at large. The impact of health hazards on the environment warrants investigation of the correlation between air pollutants and COVID-19 spread. This review provides a comprehensive overview of the effects of COVID-19 on fine particulate matter (PM), gaseous pollutants, and its potential implications on human health by considering short- and long-term impacts associated with meteorological factors in the Indian context. The current review suggests that the PM level has reduced (40–80%) in various Indian cities, which has led to a significant link in COVID-19 severity and lethality through its impact on chronic diseases, such as cardiopulmonary diseases and diabetes. In addition, the review addresses the effect of meteorological and climatic factors on COVID-19 health outcomes. In the current reviews suggested that the relationships between meteorological factors and COVID-19 transmission ceases could be affected by season, geographical scale, and latitude. Thus, the current review might be useful for the public, local authorities, and the Government to develop control measures for meteorological properties for better understanding to combat COVID-19.
Associations of mixed metals exposure with risk of cognitive impairment: A cross-sectional study in Chinese adults
Authors: Wang Jierui , Wang Weixuan , Zheng Gang , Shi Fan , Wu Shouling , Zhang Yanshu 
Published: September 2022

Abstract
Background
Associations between exposure to single metals and cognitive impairment or related outcomes have been reported in many previous studies. However, co-exposure to more than one metal is common situation. In recent years, studies on the effects of exposure to multiple metals on cognitive impairment or related outcomes have increased, but remain very limited, with a focus on populations with occupational exposure to metals, children and adolescents. The potential relationships between exposure to metal mixtures and risk of cognitive impairment in adults remain to be clarified.
Objectives
To investigate the associations of blood metal mixtures with risk of cognitive impairment.
Methods
A cross-sectional study was conducted in 1104 Chinese adults who underwent routine physical examination in the Kailuan General Hospital in Tangshan. The blood levels of lead (Pb), iron (Fe), copper (Cu), calcium (Ca), magnesium (Mg), zinc (Zn) were measured by the inductively coupled plasma mass spectrometry (ICP-MS). Multivariable logistic regression (MLR) models and Bayesian kernel function regression (BKMR) models were applied to assess the associations.
Results
A total of 218 participants (19.75%) were diagnosed with cognitive impairment. The median mini-mental state examination (MMSE) rating in cognitive impairment group (25 score) was significantly lower than that in normal cognitive function group (29score). Four metals (Pb, Fe, Cu and Mg) were positively associated with cognitive impairment in single-metal models. Pb and Cu remained significantly positive associations after adjusting for these six metals, with the odds ratios (95% confidence intervals) in the highest quartiles of 9.51 (4.41–20.54, p-trend < 0.01) and 4.87 (2.17–10.95, p-trend < 0.01), respectively. The BKMR models showed that co-exposure levels of Pb, Fe, Cu, Ca, Mg, Zn were associated with increased risk of cognitive impairment when the metal mixtures were ≥ 25th percentile compared to their medians, and Pb and Cu were the major contributors to the joint effect. In addition, interaction effects of Mg and Pb, Pb and Cu on the risk of cognitive impairment were observed.
Conclusions
Co-exposure of six metals (Pb, Fe, Cu, Ca, Mg and Zn) increased the risk of cognitive impairment in Chinese adults, with Pb and Cu likely to have greater impact. Potential interaction effects of Mg and Pb, Pb and Cu on the risk of cognitive impairment may exist.
The impact of virulence factors on divergent lifestyle scourge of Acinetobacter baumannii
Authors: Ramezanalizadeh Fatemeh , Rasooli Iraj , Owlia Parviz , Astaneh Alipour , Abdolhamidi Raziyeh 
Published: September 2022

Abstract
Acinetobacter baumannii is a multi-drug resistant pathogen with the ability to switch between planktonic and biofilm phenotypes. Although there is no vaccine against A. baumannii infections, many attempts have been made to develop vaccines using planktonic or biofilm antigens. To cover the different phenotypes of A. baumannii during growth and attachment, we combined planktonic upregulated antigens of iron receptors with biofilm upregulated antigens of pilus rods and evaluated immune responses and protective efficacies of the combined vaccine using lethal and sub-lethal murine sepsis models. The results showed that the combined vaccine elicited high IgG antibody titers and conferred protection against lethal doses of two Carbapenem- resistant high adherent A. baumannii strains. Complete bacterial clearance from all the affected tissues of the mice challenged with A. baumannii was an excellent achievement with our quadrivalent immunogen. These results demonstrate both planktonic and biofilm antigens are important during antigen selection for vaccine design.
Energy landscapes of Kodiak brown bears: A comparison of accelerometer and global positioning system derived estimates
Authors: Finnegan S. P. , Pagano A. M. , Svoboda N. J. , Schooler S. L. , Belant J. L. 
Published: September 2022

Abstract
Within optimal foraging theory animals should maximize their net energy gain while minimizing energetic costs. Energetic expenditure in wild animals is therefore key to measure proxies of fitness. Accelerometers are an effective tool to study animal movement-based energetics but retrieval of the device is usually required and often difficult. Measuring energetic expenditures using a global positioning system (GPS) approach could provide an alternative method to study energetic ecology. We compared accelerometer and GPS methods to estimate energetic expenditures in brown bears (Ursus arctos) on the Kodiak Archipelago, Alaska, USA. We then applied the GPS method to examine how intrinsic and extrinsic factors influenced brown bear movement-based daily energetic expenditures (MDEE). We predicted that bears would have greater energetic expenditures during the high food abundance period, while females with dependent young would have lower energetic expenditures due to reduced movements. We found that while the two energetic measurements differed (Wilcoxon signed rank test: V = 2116, p < 0.001), they were positively correlated (r = 0.82, p < 0.001). The GPS method on average provided 1.6 times greater energy estimates than did the accelerometer method. Brown bears had lower MDEE during periods of high food abundance, supporting optimal foraging theory. Reproductive status and age did not influence MDEE, however movement rates had a positive linear relationship with MDEE. Energetic ecology is important for understanding drivers of animal movements. A GPS-derived estimate of energetic expenditure may be suitable when accelerometer data are unavailable, but the GPS-derived estimate should be validated for the specific taxa, ecosystem, and GPS sampling rate used. Additionally, while movement-based estimates of energy expenditure can elucidate the mechanisms driving habitat use decisions, they may not fully reflect an animal’s overall energy demands. Brown bear movement-based energetic expenditure was influenced by intrinsic and extrinsic factors which highlighted the importance of access to prime foraging sites to enhance energetic efficiency.