bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation of helicase-dependent amplification with SYBR Green I for prompt naked-eye detection of bacterial contaminants in platelet products
Authors: Yamket Warangkana , Sathianpitayakul Panuwat , Santanirand Pitak , Ratthawongjirakul Panan 
Published: October 2022

Abstract
Platelet transfusions may lead to more significant risks of infection and septic transfusion reactions that can be fatal to the recipient. Platelet products should be screened to limit or detect bacterial contamination before application to patients to minimise any adverse reactions. This study aimed to develop a helicase-dependent amplification (HDA) technique targeting a universal highly conserved bacterial gene, 16S rRNA, in combination with naked-eye detection using SYBR Green I (HDA/SYBR Green I) to detect bacterial contamination in platelet products. Thirty positive samples were obtained from spiked platelet products by five transfusion-relevant bacterial strains and were screened for bacterial contamination by HDA/SYBR Green I. HDA/SYBR Green I showed an enhanced yield of bacterial contaminant detection when performed with medium to late shelf life, Day 2 of storage or later platelet products (96% sensitivity and 100% specificity). The limit of detection of HDA/SYBR Green I was 1 ng, and there was no cross-reaction with other organisms that could likely contaminate platelet products. The developed HDA/SYBR Green I assay is rapid and simplistic and only requires an easy-to-find heat box, available in general blood bank laboratories, for the amplification step. This technique is suitable for further development as an alternative method to detect bacterial contamination in platelet products in the near future.
Prevalence and determination of risk factors of Heart Failure Preserve Ejection Fraction (HFpEF) in Type 2 Diabetes Mellitus (T2DM) population in Hospital Al-Sultan Abdullah.
Authors: Zakaria Ahmad Firdaus , Hatta Sharifah Faradila Wan Muhamad , Abidin Hafisyatul Aiza Zainal , Ismail Zaliha , Ghani Rohana Abdul 
Published: October 2022

Abstract
Background: Type 2 diabetes mellitus (T2DM) and heart failure are two entities that have reached epidemic proportions worldwide. It has been shown that the prevalence of preserved ejection fraction heart failure (HFpEF) seems to be increasing due to increasing number of metabolic diseases and T2DM. However, the data on prevalence and risk factors are currently limited, particularly within our population.
Objective: The aim of this study was to determine the prevalence and associated factors of HFpEF amongst T2DM patients attending the Endocrinology & Cardiology clinics in Hospital Al-Sultan Abdullah.
Method: A cross-sectional study on patients with T2DM of more than 18 years of age, from December 2021 to May 2022 was conducted. Baseline demographic data, anthropometric measurements, echocardiography and NTproBNP levels were obtained.
Results: A total of 262 T2DM patients with a mean age of 61+5.4 years were recruited. The prevalence of HFpEF amongst the study population was 21.7% (n=57). Multiple logistic regression analysis revealed that female gender (p:0.003, OR: 3.053 (CI, 1.107:5.455), chronic kidney disease (p:0.001, OR:3.625(1.537,8.550), anaemia (haemoglobin <12g/dL) (p:<0.008 OR:2.952(1.537,6.530) and use of > 3 antihypertensive medications (p:0.005, OR:2.612(1.345,5.332) were predictors for HFpEF in the study population.
Conclusions: The prevalence of HFpEF diagnosed with abnormal echocardiography findings and elevated NTproBNP as a surrogate marker, was notable in this group of T2DM patients. Female gender, CKD, low haemoglobin, and hypertension are demonstrated to be significant predictors. This study underscores the need for early detection, initiation of the necessary treatment for HFpEF and optimisation of the associated factors.
Efficacy and risk factor analysis of femoral neck system for femoral neck fractures：a retrospective study
Authors: Yin Jingyi , Ning Duan , Zhang Congming , Zhao Guolong , Qiang Zan , Zhang Weibao , Cheng Jinhan 
Published: October 2022

Abstract
Background
To analyze the efficacy of the femoral neck system (FNS) in treating patients with femoral neck fracture (FNF) after surgery and the risk factors leading to complications such as femoral head necrosis (FHN), fracture nonunion (FN), and hardware removal (HR).
Methods
The study retrospectively included 109 patients and 109 FNF that underwent closed reduction by FNS between January 2019 and December 2021 in our single tertiary medical institution. The complications and reoperation rate of FNS and the underlying risk factors were evaluated.
Results
There are 109 patients included in the study, The overall reoperation rate is 6.4% (5 patients with FHN and 2 patients with HR) and the overall complication rate is 10.09% (6 patients with FHN, 2 patients with HR, and 3 patients with FN). For the 109 patients in the study, risk factors for complication included patient’s gender, Pauwels angle, length of femoral neck shortening. Pauwels classification, length of operation, volume of blood loss, and length of incision are also related to complications.
Conclusion
FNS is a safe option for treating FNF, the reoperation rate and the complication rate of FNS are lower than other implants. Female patients, Pauwels angle, and length of femoral neck shortening are the main risk factors of complications. Excessive pursuit of anatomical reduction is also associated with the development of complications.
Spatial clustering for echinococcosis of Yak in China based on ArcGIS during the past 4 decades
Authors: Fan Shijie , Liu Anjing , Zhao Xialing , Dan Qulamu , Tang Wenqiang , Shi Bin , Dong Hailong , Xia Chenyang 
Published: October 2022

Abstract

Background: Objective The study on the infection of hydatid disease in yaks in China provides the general epidemiological information of hydatid disease infection in yaks, which is of great value for the formulation of prevention and control strategies for hydatid disease. Method The database of yak hydatid disease infection rate was established by WPS Excel (version 11.1.0.11045-release). The provincial boundaries are vectorized by ArcGIS10.8 software, and the national and provincial boundaries and center vector maps are obtained. Taking the total number of yaks in different regions of China and the infection rate of hydatid disease of yaks in recent 40 years as attribute data, the data were matched with the basic map, and the spatial distribution map was drawn. Finally, spatial autocorrelation analysis, spatial clustering analysis, clustering and outlier analysis were used to analyze the spatial distribution of hydatid disease infection rate in county yaks in China.
Results: Except for Yunnan Province, yaks were infected with hydatid disease in the other five provinces and autonomous regions. The infection rate of hydatid disease in yaks in the five provinces has shown a downward trend in the past 40 years. The species of Echinococcus granulosus and Echinococcus multilocularis infected by yaks in five provinces were Echinococcus granulosus and Echinococcus multilocularis. There was no Echinococcus multilocularis infection in yaks in Xinjiang. The results of spatial distribution of yak hydatid disease infection in counties of China showed that there were yak hydatid disease infection in 65 counties of five provinces in China. Most of the counties with high infection rate are concentrated at the junction of eastern, western and southeastern Qinghai province, and the average infection rate of hydatid disease in yak is more than 60%. However, the average infection rate of hydatid disease in yaks in western Xinjiang Autonomous region, Tianzhu County in Gansu Province and northeastern Qinghai Province is less than 10%.
Conclusions: This is the first time based on ArcGIS to study the infection of hydatid disease in yaks in recent 40 years in China. The advantage is obvious and the spatial distribution is clear immediately.
Memory for abstract Control States does not decay with increasing retrieval delays
Authors: Schiltenwolf Moritz , Kiesel Andrea , Frings Christian , Dignath David 
Published: October 2022

Abstract
Recent studies have suggested that abstract control-states (i.e., internal attentional states independent from concrete stimuli and responses) can be stored in episodic memory and retrieved subsequently. However, the duration of such a control-state memory remains unclear. Previous research has found a quick and complete decay for stimulus-response bindings after 2000–5000 ms. Here, we tested a possible decay of control-state bindings with retrieval-delays of 2000, 3000, or 5000 ms. Five preregistered experiments used a confound-minimized prime-target task to measure the congruency sequence effect (CSE) separately for trials in which a nominally irrelevant context feature changed or repeated across trials. Analyses of the individual experiments did not result in conclusive evidence. A mega analysis integrating the data of all experiments (Ntotal = 326) replicated evidence for binding and retrieval of control-states, in that larger CSEs were found for context repetition trials. Importantly, Bayesian analysis indicated that this effect was not modulated by the length of retrieval delay. While this finding suggests that bindings of abstract control-states can be relatively robust, we also discuss possible limitations of the present research.
Evaluation of distribution practices of temperature-sensitive products in Rwanda: A descriptive cross-sectional study at a medical supply company, Rwanda
Authors: Mutabaruka Ariane , Sezirahiga Jurdas , Nyirimigabo Eric , Hahirwa Innocent , Njunwa Kato Jonas 
Published: October 2022

Abstract
Background
A strong health system cannot function without a well-designed, well-operated, and well-maintained supply chain management system. Temperature-sensitive products within the supply chain are products that encompass pharmaceutical goods or products, which, when not stored or transported within predefined environmental conditions and/or within predefined time limits, are degraded and rendered not fully effective. The objective of this study was to evaluate the compliance to the good distribution practices of temperature-sensitive products in Rwanda.
Methods
A cross-sectional study was conducted at a medical supply company in Rwanda and involved 30 staff members managing temperature-sensitive products. The staff came from the Quality Assurance and quality control unit, Warehouse and logistics unit, Sales and marketing unit, Distribution unit, as well as some branch directors. Interviews were conducted and historical data on recorded temperature and humidity levels in storerooms and refrigerators/freezers as well as transit temperature data from representative district branches were collected for a period of three years (2017–2019). All data were analyzed using SPSS version 25. For compliance, temperature and relative humidity were compared with international standards.
Results
It was found that the Standard Operating Procedures were available but not updated to ensure compliance. The scarcity of trained staff was found to be the main factor hindering adherence to standards, hence monitoring of temperature and relative humidity sometimes had loopholes that need to be addressed. This study also discovered that adherence to recommended temperature ranges for the products was compromised by inconsistency in recording and reporting temperature and humidity, and a lack of temperature monitoring tools and qualified equipment.
Conclusions
The study revealed the weaknesses that exist in the process of distribution of temperature-sensitive products at the medical supply company, which mainly, are attributed to paucity of trained staff. Strategic planning for short, medium, and long-term remedies will require that all parties involved in the management of these products work together towards upholding the standard.
Effects of whole-body sorcin knockout on body weight and hypothalamic ER-stress in C57BL/6 male mice fed a westernized diet
Authors: Parks Steven Z. , Rutter Guy A. , Leclerc Isabelle 
Published: October 2022

Abstract
Background
Soluble Resistance Related Calcium Binding Protein (sorcin) is a calcium (Ca2+) binding protein which has been shown to play a role in maintaining intracellular endoplasmic reticulum (ER) Ca2+ stores and lowering ER stress. Recently, our lab has demonstrated that sorcin expression was downregulated in the islets of Langerhans of mice fed a high-fat diet or in human islets incubated with the saturated fatty acid palmitate. We also showed that overexpression of sorcin under control of the rat insulin promoter (RIP7) in C57BL/6J mice, or whole body sorcin deletion in 129S1/SvImJ mice, improves or impairs insulin secretion and pancreatic β-cell function respectively. The mechanisms behind this beneficial role of sorcin in the pancreatic β-cell might depend on protection against lipotoxic endoplasmic reticulum (ER) stress through improved ER Ca2+ dynamics and activation of the Activating Transcription Factor 6 (ATF6) branch of the unfolded protein response (UPR). Whether sorcin is also implicated in hypothalamic ER stress during the progression of obesity is unknown.
Aim
To investigate a potential role of sorcin in hypothalamic ER stress, leptin resistance, hyperphagia and obesity.
Methods
Whole-body sorcin null mice, backcrossed onto the C57BL/6J genetic background, were used. Body weight, food intake and EchoMRI body composition were measured in vivo whereas qRT-PCR analysis of sorcin and ER stress markers expression were performed on the arcuate nucleus of the hypothalamus. Leptin signalling through STAT3 phosphorylation was measured by Western blots on sorcin-null HEK293 cells, engineered by CRISPR/Cas9, and transfected with leptin receptor (LepRb).
Results
Sorcin expression was not influenced in the arcuate nucleus (ARC) of the hypothalamus by diet-induced obesity. Whole-body sorcin ablation did not cause ARC ER stress nor changes in body weight, body composition or food intake in C57BL/6 male mice exposed to a high-fat, high-sugar diet. STAT3 phosphorylation (Y705) in response to leptin was not impaired in sorcin-null HEK293 cells.
Conclusion
In our model, whole body sorcin ablation did not increase hypothalamic ER stress nor influenced food intake or body weight.
Analysis of cytokine mRNA expression during Zika infection in Mesocricetus auratus
Authors: Mendes Wailla Rafaela Barros , de Melo Karla Fabiane Lopes , de Luna Francisco Canindé Ferreira , Santos Carolina Ramos dos , Franco Edna Cristina dos Santos , Vasconcelos Pedro Fernando da Costa , Casseb Samir Mansour Moraes 
Published: October 2022

Abstract
The Zika virus (VZIK) is a Flavivirus of the Flaviviridae family. With the ability to infect neural progenitor cells, microcephaly is the most serious and irreversible neurological complication associated with ZIKV. In this work, we evaluated the mRNA expression of cytokines involved in the inflammatory process that occurred during Zika virus infection in Mesocricetus auratus. The samples came from the real tissue of animals infected through sexual transmission and comprising 3 different groups of animals (TS-01, TS-02 and ZP-01).The project started with the extraction of viral RNA and mRNA from the kidneys of ZIKV-infected animals. After this step, RT-qPCR was carried out to quantify both the viral load and the expression of cytokines associated with the inflammatory process that occurred during the ZIKV infection. As can be seen in our results, this work corroborates different studies that demonstrate that ZIKV can infect and cause an inflammatory response to the kidneys, even causing a high viral load.
Friction to unconfined compression strength correlation for evaluating capacity of weak rock foundation sockets
Authors: MANOJ SUJATHA MANOJ , Choudhury Deepankar , Poulos Harry , AlZaylaie Marwan 
Published: October 2022

Abstract
Historically, deep foundations in weak rock have been designed as friction elements using frictional resistance (fs) calculated from the unconfined compressive strength (UCS) of rock. Most of the published correlations of fs to UCS were developed based on load tests on low-capacity piles in specific geological conditions, using UCS values not necessarily representative over the test depth. There is a large variation in foundation design depths calculated using these correlations. This paper presents a correlation between fs and UCS of weak rock, developed using data from 44 bidirectional load tests from high-capacity deep foundations in weak rocks. The dataset used in this study, is one of the largest used for weak rocks, with high test loads in the range of 100 to 320MN and the depth of foundations mostly in the range of 20 to 87m below ground level. Bi-directional load test data from La Maison tower site is then simulated in Plaxis, and ultimate skin friction developed is compared against the skin friction calculated using the new corelation. The actual ground profile and foundation layout of La Maison tower is then modelled in Plaxis with the required foundation depth derived using the recommended corelation, to check serviceability limits. The resulting maximum settlements are found to be well within the acceptable limits. The correlation factor of 0.5 between fs and UCS is thus recommended for estimating rock socket friction for design of deep foundations in weak carbonate rock formations.
Exploring the purpose of educational supervision through the lens of Proctor’s model: pharmacy professionals’ perceptions
Authors: Styles Michelle , Schafheutle Ellen , Willis Sarah , Shaw Matthew 
Published: October 2022

Abstract
Background
Educational supervision plays a vital role in postgraduate medical education and more recently in pharmacy and advanced clinical practitioner training in England. Proctor’s three-function model of clinical supervision (consisting of normative, formative, and restorative functions) is assumed to also apply to educational supervision, but this has not been tested empirically. The aim of this study was to establish pharmacy professionals’ perceptions of the purpose of educational supervision using Proctor’s model of clinical supervision as its lens.
Methods
Using a concurrent embedded mixed methods design, data were collected using a validated 25-item online survey and respondents were invited to add comments explaining their ratings. A census survey was sent to all learners enrolled on a postgraduate training pathway for pharmacy professionals working in primary care in England. Principal components analysis (PCA) was used to determine the number of latent variables underlying the survey and framework analysis was used to identify themes from comments and aid interpretation of quantitative findings.
Results
187 pharmacy professionals responded (response rate 20.7%). PCA extracted three factors explaining 71.5% of the total variance. Factor 1 corresponded with survey items linked to the formative function of Proctor’s model, while factor 2 corresponded with survey items linked to the restorative function. No items corresponded with the normative function. Framework analysis of comments identified two themes: support for learning, which corresponded with factor 1; and personal support, which corresponded with factor 2.
Conclusions
This study identified that pharmacy professionals perceived educational supervision to perform two functions, formative and restorative, but did not perceive it to perform a normative function. Educational supervision has the potential to support allied health professionals advancing their roles and we suggest the need for more research to clarify its purpose.
Epilepsy Treatment Outcome, Adherence to Anti-seizure Medications and Predicting factors at the chronic care facility in Jimma Universtiy Medical Center: Cross-sectional study
Authors: Shuma Firafan , Terefe Behailu , Tekassa Tamirat 
Published: October 2022

Abstract

The aim of this study was to assess epilepsy treatment outcome, adherence to Anti-seizure medication (ASM), and its predictors among epileptic patients on follow-up at the chronic care unit of Jimma University Medical Center with a total of 168 epileptic patients enrolled in the study. Data was collected by data collectors using data abstraction formats, then entered and analyzed using SPSS version 26.0. Multiple logistic regression analysis was performed to identify the association between dependent and independent variable. In this study, 132(78.6%) patients were adherent to their ASMs. Seizure recurrence was identified in 120(71.4%) patients. Rural residence (AOR = 6.42, 95% CI: 1.32, 31.28, P-value = 0.02), chronic ASM therapy for above five years (AOR = 20.86, 95% CI: 2.66, 163.77, P-value = 0.00), and complaint of ASM-associated undesirable effect (AOR = 13.51, 95% CI: 2.72, 67.26, P-value = 0.00) significantly increased the probability of seizure recurrence. On the other hand, the presence of seizure triggering factor(s) (AOR = 0.12, 95% CI: 0.02, 0.64, P-value = 0.01) decreased the probability of seizure recurrence by 88%.
An Immunogenic Cell Death-Related Classification Predicts Prognosis and Response to Immunotherapy in Hepatocellular carcinoma
Authors: Wang Jianguo , Shen Bingbing , Liu Xinyuan , Zhang Shihang , Yang Chengxiao , Zheng Qingyuan , Hao Yiqun , Jiang Jianxin 
Published: October 2022

Abstract
Hepatocellular carcinoma (HCC) is one of the world's malignant tumors with high mortality and a worse prognosis. Immunogenic cell death is caused by tumor cells stimulated by stressors to display or release Danger Associated Molecular Patterns (DAMPs)that attract and activate antigen-presenting cells. It would be advantageous to identify biomarkers associated with Immunogenic cell death(ICD) and stratify patients according to their benefits from ICD immunotherapy. Here, we identified two subtypes ICD-related by consensus clustering. ICD-high Subtype has an excellent clinical outcome, abundant immune cell infiltration, and highly active immune response signaling. In addition, we developed and validated an ICD-related risk model that predicts HCC patients' survival and correlates with the tumor immune microenvironment. In conclusion, we have established a new classification system for HCC based on ICD signatures. This stratification has significant clinical outcomes for estimating prognosis and immunotherapy in HCC patients.
Corrosion Behavior of AZ91/TiC/Al 2 O 3 Hybrid Composites
Authors: Singh Kamal Kant , Professor Dharamvir Mangal Research Assistant 
Published: October 2022

Abstract
In the current research, the corrosion behaviors of AZ91 Mg-hybrid composites amalgamated with titanium carbide and alumina reinforcing particles have been investigated. AZ91 Mg-hybrid composites have been developed by using the bottom pouring vacuum-based stir casting process to attain the homogeneous distribution of reinforcing particles in the AZ91-Mg matrix. The corrosion behavior of AZ91-hybrid composites has been evaluated by using corrosion potential (OCP) and potential dynamic polarization scans, as well as friction coefficient, has been deduced to examine the wear behavior of hybrid composites in sodium chloride (3.5%) solution. However, before and after each corrosion and wear test of AZ91-hybrid composites samples, the metallographic structures have been examined by using a scanning microscopic setup. The corrosive results reveal that the corrosion rate of the AZ91-Mg matrix has been greater than AZ91/TiC/Al2O3 composites. The results also confirm that the corrosion resistance increases with an increase in wt. % of TiC and Alumina. The prime reason for to increase in the corrosion rate is due to the increase in the number of matrix/reinforcement interfaces. However, the friction coefficient and wear rate of AZ91 Mg-hybrid composites rose with the increase in TiC wt. %. The type of wear mechanism of AZ91 Mg-hybrid composites has been demonstrated that initially abrasive wear has been observed but later it predominantly transforms to both (i.e. adhesive and abrasive wear) with an increase in TiC wt. %.