bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural network of superiority illusion predicts the level of dopamine in striatum
Authors: Yahata Noriaki , Isato Ayako , Kimura Yasuyuki , Yokokawa Keita , Zhang Ming-Rong , Ito Hiroshi , Suhara Tetsuya , Higuchi Makoto , Yamada Makiko 
Published: February 2022

AbstractIn evaluating the personality attributes and performance of the self, people are inclined to view themselves superior to others, a phenomenon known as superiority illusion (SI). This illusive outlook pervades people’s thoughts, creating hope for the future and promoting mental health. Although a specific cortico-striatal functional connectivity (FC) under dopaminergic modulation was previously shown to be implicated in SI, the underlying whole-brain mechanisms have remained unclarified. Herein, to reveal the neural network subserving individual’s SI, we conducted a data-driven, machine-learning investigation to explore the resting-state FC network across the whole brain. Using the locally-acquired resting-state functional magnetic resonance imaging data (n = 123), we identified a set of 15 FCs most informative in classifying individuals with higher-versus lower-than-average levels of SI in evaluating positive trait words (area under the curve [AUC] = 0.81). Among the 15 FCs, the contribution level to the classification was 11% by the previously-highlighted cortico-striatal FC alone, but 60% by the encompassing cortico-limbico-striatal network cluster. A newly-identified, cortico-thalamic FC and another FC cluster also demonstrated substantial contribution. The classification accuracy was generalized into an independent cohort (n = 36; AUC = 0.73). Importantly, using the same set of 15 FCs, we achieved prediction on an individual’s level of striatal dopamine D2 receptor availability (Pearson correlation, r = 0.46, P = 0.005). This is the first successful identification of the whole-brain neural network that simultaneously predicts the behavioral manifestation and molecular underpinning of an essential psychological process that promotes well-being and mental health.Significance StatementSuperiority illusion (SI) is a basic self-referential framework that pervades people’s thoughts and promotes well-being and mental health. An aberrant form of SI has been reported in psychiatric conditions such as depression. Our hypothesis-free, data-driven investigation revealed the spatially-distributed neural network that for the first time achieved prediction on an individual’s levels of SI and the striatal dopaminergic transmission simultaneously. In principle, this multiple-biological-layer framework can be applicable to any behavioral trait to establish a link with its underlying neural network and neurochemical properties, which could quantitatively present the relation of its aberrant form with the pathophysiology of neuropsychiatric disorders. Future clinical research may aid in deriving a diagnostic biomarker for examining the related behavioral and neurochemical characteristics within individuals.
The extinction time under mutational meltdown
Authors: Lansch-Justen Lucy , Cusseddu Davide , Schmitz Mark A. , Bank Claudia 
Published: February 2022

AbstractMutational meltdown describes an eco-evolutionary process in which the accumulation of deleterious mutations causes a fitness decline that eventually leads to the extinction of a population. Possible applications of this concept include medical treatment of RNA virus infections based on mutagenic drugs that increase the mutation rate of the pathogen. To determine the usefulness and expected success of such an antiviral treatment, estimates of the expected time to mutational meltdown are necessary. Here, we compute the extinction time of a population under high mutation rates, using both analytical approaches and stochastic simulations. Extinction is the result of three consecutive processes: (1) initial accumulation of deleterious mutations due to the increased mutation pressure; (2) consecutive loss of the fittest haplotype due to Muller’s ratchet; (3) rapid population decline towards extinction. We find accurate analytical results for the mean extinction time, which show that the deleterious mutation rate has the strongest effect on the extinction time. We confirm that intermediatesized deleterious selection coefficients minimize the extinction time. Finally, our simulations show that the variation in extinction time, given a set of parameters, is surprisingly small.
An in vitro method for inducing titan cells reveals novel features of yeast-to-titan switching in the human fungal pathogen Cryptococcus gattii
Authors: Saidykhan Lamin , Correia Joao , Romanyuk Andrey , Desanti Guillaume E. , Taylor-Smith Leanne , Makarova Maria , Ballou Elizabeth R. , May Robin C. 
Published: February 2022

AbstractCryptococcosis is a potentially lethal fungal infection of humans caused by organisms within the Cryptococcus neoformans/gattii species complex. Whilst C. neoformans is a relatively common pathogen of immunocompromised individuals, C. gattii is capable of acting as a primary pathogen of immunocompetent individuals. Within the host, both species undergo morphogenesis to form titan cells: exceptionally large cells that are critical for disease establishment. To date, the induction, defining attributes, and underlying mechanism of titanisation have been mainly characterized in C. neoformans. Here, we report the serendipitous discovery of a simple and robust protocol for in vitro induction of titan cells in C. gattii. Using this in vitro approach, we reveal a remarkably high capacity for titanisation within C. gattii, especially in strains associated with the Pacific Northwest Outbreak, and characterise strain-specific differences within the clade. In particular, this approach demonstrates for the first time that cell size changes, DNA amplification, and budding are not always synchronous during titanisation. Interestingly, however, exhibition of these cell cycle phenotypes was correlated with genes associated with cell cycle progression including CDC11, CLN1, BUB2, and MCM6. Finally, our findings reveal exogenous p-Aminobenzoic acid to be a key inducer of titanisation in this organism. Consequently, this approach offers significant opportunities for future exploration of the underlying mechanism of titanisation in this genus.
A cell fate decision map reveals abundant direct neurogenesis in the human developing neocortex
Authors: Coquand Laure , Macé Anne-Sophie , Farcy Sarah , Avalos Clarisse Brunet , Cicco Amandine Di , Lampic Marusa , Bessières Betina , Attie-Bitach Tania , Fraisier Vincent , Guimiot Fabien , Baffet Alexandre 
Published: February 2022

AbstractThe human neocortex has undergone strong evolutionary expansion, largely due to an increased progenitor population, the basal radial glial (bRG) cells. These cells are responsible for the production of a diversity of cell types, but the successive cell fate decisions taken by individual progenitors remains unknown. Here, we developed a semi-automated live/fixed correlative imaging method to generate a map of bRG cell division modes in early fetal tissue and cerebral organoids. Through the analysis of over 1,000 dividing progenitors, we show that bRG cells undergo abundant symmetric amplifying divisions, followed by frequent direct neurogenic divisions, bypassing intermediate progenitors. These direct neurogenic divisions are more abundant in the upper part of the subventricular zone. We furthermore demonstrate asymmetric Notch activation in the self-renewing daughter cells, independently of basal fiber inheritance. Our results reveal a remarkable conservation of fate decisions in cerebral organoids, supporting their value as models of early human neurogenesis.
Positive strand RNA viruses differ in the constraints they place on the folding of their negative strand
Authors: Herod Morgan R. , Ward Joseph , Tuplin Andrew , Harris Mark , Stonehouse Nicola J. , McCormick Christopher J. 
Published: February 2022

ABSTRACTGenome replication of positive strand RNA viruses requires the production of a complementary negative strand RNA that serves as a template for synthesis of more positive strand progeny. Structural RNA elements are important for genome replication, but while they are readily observed in the positive strand, evidence of their existence in the negative strand is more limited. We hypothesised that this was due to viruses differing in their capacity to allow this latter RNA to adopt structural folds. To investigate this, ribozymes were introduced into the negative strand of different viral constructs; the expectation being that if RNA folding occurred, negative strand cleavage and suppression of replication would be seen. Indeed this was what happened with hepatitis C virus (HCV) and feline calicivirus (FCV) constructs. However, little or no impact was observed for chikungunya virus (CHIKV), human rhinovirus (HRV), hepatitis E virus (HEV) and yellow fever virus (YFV) constructs. Reduced cleavage in the negative strand proved to be due to duplex formation with the positive strand. Interestingly, ribozyme-containing RNAs also remained intact when producedin vitroby the HCV polymerase, again due to duplex formation. Overall, our results show that there are important differences in the conformational constraints imposed on the folding of the negative strand between different positive strand RNA viruses.
The Collaborative Cross strains and their founders vary widely in cocaine-induced behavioral sensitization
Authors: Schoenrock SA , Gagnon L , Olson A , Leonardo M , Phillip VM , He H , Jentsch JD , Chesler EJ , Tarantino LM 
Published: February 2022

AbstractCocaine use and overdose deaths attributed to cocaine have increased significantly in the United States in the last 10 years. Despite the prevalence of cocaine use disorder (CUD) and the personal and societal problems it presents, there are currently no approved pharmaceutical treatments. The absence of treatment options is due, in part, to our lack of knowledge about the etiology of CUDs. There is ample evidence that genetics plays a role in increasing CUD risk but thus far, very few risk genes have been identified in human studies. Genetic studies in mice have been extremely useful for identifying genetic loci and genes, but have been limited to very few genetic backgrounds, leaving substantial phenotypic and genetic diversity unexplored.Herein we report the measurement of cocaine-induced behavioral sensitization using a 19-day protocol that captures baseline locomotor activity, acute locomotor response to cocaine and locomotor sensitization across 5 exposures to the drug. These behaviors were measured in 51 genetically diverse yet tractable Collaborative Cross (CC) strains along with their inbred founder strains. The CC was generated by crossing 8 genetically diverse inbred strains such that each inbred CC strain has genetic contributions from each of the founder strains. Inbred CC mice are infinitely reproducible and provide a stable, yet diverse genetic platform on which to study the genetic architecture and genetic correlations among phenotypes.We have identified significant differences in cocaine locomotor sensitivity and behavioral sensitization across the panel of CC strains and their founders. We have established relationships among cocaine sensitization behaviors and identified extreme responding strains that can be used in future studies aimed at understanding the genetic, biological and pharmacological mechanisms that drive addiction-related behaviors. Finally, we have determined that these behaviors exhibit relatively robust heritability making them amenable to future genetic mapping studies to identify addiction risk genes and genetic pathways that can be studied as potential targets for the development of novel therapeutics.
Temporal and spatial auxin responsive networks in maize primary roots
Authors: McReynolds Maxwell R. , Dash Linkan , Montes Christian , Draves Melissa A. , Lang Michelle G. , Walley Justin W. , Kelley Dior R. 
Published: February 2022

AbstractAuxin is a key regulator of root morphogenesis across angiosperms. To better understand auxin regulated networks underlying maize root development we have characterized auxin responsive transcription across two time points (30 and 120 minutes) and four regions of the primary root: the meristematic zone, elongation zone, cortex, and stele. Hundreds of auxin-regulated genes involved in diverse biological processes were quantified in these different root regions. In general, most auxin regulated genes are region unique and are predominantly observed in differentiated tissues compared to the root meristem. Auxin gene regulatory networks (GRNs) were reconstructed with these data to identify key transcription factors that may underlie auxin responses in maize roots. Additionally, Auxin Response Factor (ARF) subnetworks were generated to identify target genes which exhibit tissue or temporal specificity in response to auxin. These networks describe novel molecular connections underlying maize root development and provide a foundation for functional genomic studies in a key crop.
Clathrin mediates both internalization and vesicular release of triggered T cell receptor at the immunological synapse through distinct adaptors
Authors: Kvalvaag Audun , Céspedes Pablo F , Valvo Salvatore , Saliba David G , Kurz Elke , Korobchevskaya Kseniya , Dustin Michael L 
Published: February 2022

AbstractLigation of the T cell receptor (TCR) to peptide-MHC complexes initiates signaling leading to T cell activation. Regulation of T cell responses also requires mechanisms to stop this signaling and to downregulate surface expression of the receptor. T cells achieve this both by TCR internalization and by releasing TCR loaded vesicles directly from the plasma membrane. How these distinct fates are coordinated is unknown. Here we show that clathrin is recruited to TCR microclusters by HRS and STAM2 and that this process is essential for TCR release. Subsequently, EPN1 recruits clathrin to the remaining antigen conjugated TCRs to enable a late wave of TCR endocytosis. With these results we demonstrate two clathrin-dependent mechanisms and show how the clathrin machinery participates in membrane evagination and invagination depending upon the adaptor recruiting it. These sequential mechanisms mediate bi-directional membrane exchange at the immunological synapse, providing a scaffold for critical communication.
Knock-out of CmNAC-NOR affects melon climacteric fruit ripening
Authors: Bin Liu , Domingo Miguel Santo , Mayobre Carlos , Martín-Hernández Ana Montserrat , Pujol Marta , Garcia-Mas Jordi 
Published: February 2022

ABSTRACTFruit ripening is an important process that affects fruit quality. In melon, ETHQV6.3, a QTL involved in climacteric ripening regulation, was previously found to be encoded by CmNAC-NOR, a homologue of the tomato NOR gene. To further investigate CmNAC-NOR function we have obtained two CRISPR/Cas9 mediated mutants (nor-3 and nor-1) in the climacteric Védrantais background. nor-3, containing a 3-bp deletion altering the NAC domain A, resulted in ~8 days delay of ripening without affecting fruit quality. In contrast, nor-1 contained a 1-bp deletion resulting in a fully disrupted NAC domain, which completely blocked climacteric ripening. nor-1 fruits did not produce ethylene, abscission layer was not formed and there was no external color change. Additionally, volatile components were dramatically altered, seeds were not well developed and flesh firmness was also altered. nor-1 allele in heterozygosis showed ~20 days delay of fruit ripening. Our results provide new information regarding the function of CmNAC-NOR in melon fruit ripening, suggesting that it as a potential target to modulate shelf life in climacteric melon commercial varieties.
Bank profitability in Sub-Saharan Africa: does economic globalization matter?
Authors: Yakubu Ibrahim Nandom , Bunyaminu Alhassan 
Published: February 2022

PurposeThis study aims to examine the impact of economic globalization on bank profitability in Sub-Saharan Africa.Design/methodology/approachThe empirical analysis is based on panel data of banks spanning 2008–2016. Relying on the KOF Globalization Index, the study uses financial globalization and trade globalization as measures of economic globalization. The authors employ the system generalized method of moments technique to establish the relationship between economic globalization and bank profitability while controlling for the effect of bank-specific and macroeconomic factors.FindingsThe results show a negative significant effect of financial and trade globalization on bank profitability, signifying the intense competition of banks in Sub-Saharan Africa accelerated by globalization. The negative effect of economic globalization holds irrespective of the indicator of bank profitability. Bank size exerts a significant effect on profitability though the impact is negative for return on equity measure. The findings further reveal a positive significant impact of GDP growth and inflation on profitability.Originality/valueThis paper presents a pioneering work on the impact of economic globalization on bank profitability in the Sub-Saharan African context per the researchers' knowledge.
Analysis and comparison of flow velocities and in-cylinder temperature distributions to quantify oxides of nitrogen in a compression ignition engine using diesel and biodiesel fuels
Authors: Ahmed Munir , Shakaib Muhammad , Siddiqui Mubashir Ali 
Published: February 2022

Purpose
Combustion of fuel with oxidizer inside a combustion chamber of an internal combustion engine forms inevitable oxides of nitrogen (NOx) due to high temperature at different locations of the combustion chamber. This study aims to quantify NOx formed inside the combustion chamber using two fuels, a conventional diesel (n-heptane) and a biodiesel (methyl oleate).

Design/methodology/approach
This research uses a computational fluid dynamics simulation of chemically reacting fluid flow to quantify and compare oxides of nitrogen (NOx) in a compression ignition (CI) engine. The study expends species transport model of ANSYS FLUENT. The simulation model has provided the temperature profile inside the combustion chamber, which is subsequently used to calculate NOx using the NOx model. The simulation uses a single component hydrocarbon and oxygenated hydrocarbon to represent fuels; for instance, it uses n-heptane (C7H16) for diesel and methyl-oleate (C19H36O2) for biodiesel. A stoichiometric air–fuel mixture is used for both fuels. The simulation runs a single cylinder CI engine of 650 cm3 swept volume with inlet and exhaust valves closed.

Findings
The pattern for variation of velocity, an important flow parameter, which affects combustion and subsequently oxides of nitrogen (NOx) formation at different piston locations, is similar for the two fuels. The variations of in-cylinder temperature and NOx formation with crank angles have similar patterns for the fuels, diesel and biodiesel. However, the numerical values of in-cylinder temperature and mass fraction of NOx are different. The volume averaged static peak temperatures are 1,013 K in case of diesel and 1,121 K in case of biodiesel, while the mass averaged mass fractions of NOx are 15 ppm for diesel and 141 ppm for biodiesel. The temperature rise after combustion is more in case of biodiesel, which augments the oxides of nitrogen formation. A new parameter, relative mass fraction of NOx, yields 28% lower value for biodiesel than for diesel.

Originality/value
This work uses a new concept of simulating simple chemical reacting system model to quantify oxides of NOx using single component fuels. Simplification has captured required fluid flow data to analyse NOx emission from CI engine while reducing computational time and expensive experimental tests.
Transferable learning about patient and public involvement and engagement in gambling support services from health and social care: findings from a narrative review and workshop with people with lived experience
Authors: Norrie Caroline , Bramley Stephanie , Lipman Valerie , Manthorpe Jill 
Published: February 2022

PurposeThe involvement of patients or members of the public within public health, health and social care and addictions services is growing in the UK and internationally but is less common in gambling support services. The purpose of this study was to explore Patient and Public Involvement (PPI) infrastructures and engagement channels used in health and care services and debate their transferability to the gambling support sector (including research, education and treatment).Design/methodology/approachA narrative review examined data from six English language electronic databases, NHS evidence and grey literature covering the period 2007–2019. We identified 130 relevant items from UK literature. A workshop was held in London, England, with people with lived experience of gambling harm to seek their views on and applicability of the review findings to gambling services.FindingsSynthesis of literature and workshop data was undertaken. Main themes addressed “What works” in relation to: building infrastructures and organising involvement of people with lived experience; what people want to be involved in; widening participation and sustaining involvement and respecting people with lived experience.Practical implicationsExamination of the literature about involvement and engagement of patients, service users and the public in public health, health and social care and addiction services provides potentially useful examples of good practice which may be adopted by gambling services.Originality/valueThe involvement of people with lived experience of gambling harms in gambling support services is under-explored, with little published evidence of what constitutes good practice amongst self-organising groups/networks/grassroots organisations or rights-based/empowerment-based approaches.
Work and non-work life of R&D professionals during Covid-19: a cultural perspective
Authors: Jayaraman Ravisankar , Mishra Sushanta Kumar 
Published: February 2022

PurposeThe purpose of this study is to comprehend the approach and adaptation of research and development (R&D) professionals in managing work and non-work life during Covid-19. Besides, it investigates the influence of organizational culture on extra-role behavior both within and outside the organizational boundaries during uncertainty.Design/methodology/approachBeing an exploratory study, the data were generated through the in-depth personal interview from 23 respondents of the Indian Space Research Organization. Besides, the study also used data from secondary sources. The authors followed thematic analysis for eliciting themes from the text data.FindingsPrimarily, it found that the organizational culture may influence employees to perform extra-role behavior within and outside the organizational boundaries during the pandemic. Besides, it found that R&D professionals are adaptive toward the work from home culture.Research limitations/implicationsThe qualitative study is based on in-depth interviews of 23 R&D professionals during the Covid-19. Future studies may conduct a larger-scale quantitative study to generalize the findings. Implications for future research on hybrid work culture are discussed.Practical implicationsThis study hints that employers need to move from a short-term transactional approach to a long-term cultural approach to navigate extreme uncertainty. It also highlights reorientation of human resource professionals in managing workplace evolution.Originality/valueThe study extends the literature on organizational culture by attributing culture for the extra-role behaviors of R&D professionals beyond the organizational boundary and navigating uncertainty.