bluuebunny/crossref_metadata_2025_split huggingface dataset:
circSKIL promotes osteoblastic differentiation of periodontal ligament cells by sponging miR‐532‐5p to activate Notch signaling
Authors: Zhong Xiaohuan , Wang Huixin 
Published: September 2022

AbstractBackground and objectivePeriodontal ligament cells (PDLCs) possess the capacity to differentiate into a variety of cell types to benefit periodontal regeneration. In this study, we examined the circSKIL/miR‐532‐5p/Notch1 axis in controlling the osteoblastic differentiation of PDLCs.MethodsPrimary human PDLCs (hPDLCs) were isolated and induced to differentiate into osteoblasts. Osteogenic responses were assessed for the expressions of osteoblast‐related marker proteins (including alkaline phosphatase (ALP), osteocalcin (OCN), bone morphogenetic protein‐2 (BMP2), and runt‐related transcription factor 2 (RUNX2) by RT‐PCR. The formation of mineralized nodules was examined by Alizarin Red S (ARS) staining and ALP activity. Expressions of circSKIL, miR‐532‐5p, and Notch1 were measured by RT‐PCR and western blotting, and their regulations by combining bioinformatic analysis and luciferase reporter assay. Notch signaling was assessed for the expressions of hairy and enhancer of split‐1 (HES1) and Notch intracellular domain (NICD).ResultsDuring osteoblastic differentiation of hPDLCs, circSKIL, and Notch1 were up‐regulated, while miR‐532‐5p down‐regulated. By sponging miR‐532‐5p, circSKIL activated Notch signaling, increasing levels of Notch1, HES1, and NICD. Functionally, knocking down circSKIL or overexpressing miR‐532‐5p inhibited osteoblastic differentiation of PDLCs, down‐regulating ALP, OCN, BMP2, and RUNX2, and reducing ARS staining or ALP activity. The impacts of circSKIL knockdown were rescued by miR‐532‐5p inhibitor or overexpressing Notch1, while those caused by up‐regulating miR‐532‐5p were reversed by overexpressing Notch1.ConclusionBy targeting miR‐532‐5p and up‐regulating Notch1, circSKIL critically controls osteoblastic differentiation of hPDLCs. Therefore, modulating this axis may maximize the differentiation of PDLCs into osteoblasts and benefit periodontal regeneration.
Valuing tacit nursing knowledge during the COVID‐19 pandemic
Authors: Orr Elizabeth , Jack Susan M. , Campbell Karen , Strohm Sonya 
Published: September 2022

AbstractPublic health nurses in Ontario, Canada, support the healthy growth and development of children across the province through a variety of programs including home visits for pregnant individuals and families with young children. During the COVID‐19 global pandemic the needs of families increased while access to health and social services decreased. During this time, home visiting teams closely involved in supporting families also experienced staff redeployment to support pandemic efforts (e.g., case and contact management, vaccinations) and changes to the nature of home visiting work, including shifts to remote or virtual service delivery. To support nursing practice in this new and evolving context, a framework for capturing and sharing the tacit or how‐to knowledge of public health nurses was developed. A valuing of this type of knowledge for informing future public health nursing practice – well beyond the pandemic response – was recognized as a pandemic silver lining when reflecting on two years of supporting home visiting teams in our province.
Impact of helminth–microbiome interactions on childhood health and development—A clinical perspective
Authors: Piazzesi Antonia , Putignani Lorenza 
Published: September 2022

AbstractHumans have co‐existed with parasites for virtually the entirety of our existence as a species. Today, nearly one third of the human population is infected with at least one helminthic species, most of which reside in the intestinal tract, where they have co‐evolved alongside the human gut microbiota (GM). Appreciation for the interconnected relationship between helminths and GM has increased in recent years. Here, we review the evidence of how helminths and GM can influence various aspects of childhood development and the onset of paediatric diseases. We discuss the emerging evidence of how many of the changes that parasitic worms inflict on their host is enacted through gut microbes. In this light, we argue that helminth‐induced microbiota modifications are of great importance in both facing the global challenge of overcoming parasitic infections, and in replicating helminthic protective effects against inflammatory diseases. We propose that deepening our knowledge of helminth–microbiota interactions will uncover novel, safer and more effective therapeutic strategies in combatting an array of childhood disorders.
Narrative strategies for emerging disruptive technologies: A case study of Blockchain for Europe
Authors: Kanol Direnç 
Published: September 2022

AbstractHow do interest groups advocating for emerging disruptive technologies use narrative strategies? I argue that the characteristics of this policy area should define how actors use policy narratives and narrative strategies. Interest groups that strive for innovation, support, and development of emerging technologies should mention benefits of policies and policy proposals more than costs. In addition, they should use the hero character more than the villain and victim characters. This article studies the Twitter messages of Blockchain for Europe, the most prevalent business association representing cryptocurrency companies in the EU and finds evidence for these hypotheses. I show how novel interest groups use strategies to influence policies targeting emerging disruptive technologies like crypto‐assets. Also, I contribute to the Narrative Policy Framework research by demonstrating how narrative strategies can be influenced by the context surrounding specific issues.Related ArticlesChang, Katherine T., and Elizabeth A. Koebele. 2020. “What Drives Coalitions' Narrative Strategy? Exploring Policy Narratives around School Choice.” Politics & Policy 48(4): 618–57. https://doi.org/10.1111/polp.12367.O'Donovan, Kristin T. 2018. “Does the Narrative Policy Framework Apply to Local Policy Issues?” Politics & Policy 46(4): 532–70. https://doi.org/10.1111/polp.12265.Shanahan, Elizabeth A., Mark K. McBeth, and Paul L. Hathaway. 2011. “Narrative Policy Framework: The Influence of Media Policy Narrative on Public Opinion.” Politics & Policy 39(3): 373–400. https://doi.org/10.1111/j.1747‐1346.2011.00295.x.
Race‐based affirmative action for higher education in Brazil: Impact assessment on performance, time, and delay in completion*
Authors: dos Santos Priscila S. , Becker Kalinca L. , de Oliveira Sibele V. 
Published: September 2022

AbstractThis paper aims to investigate the impact of race‐based affirmative action for higher education in Brazil, through the effects of applying under a quota versus the open system for Black, Brown, and Indigenous people, on academic performance, time, and delay in completion. The data used were obtained by the survey of the National Student Performance Examination (Enade) from 2016 to 2018. Through the propensity score matching methodologies, entropy balancing, empirical quantile regression strategy, and Lewbel method, a small statistically significant negative impact on students' performance was observed. Significant effects on time and delay in completion were not found in most of the estimators used. We also observed that older students and students who are male, lack, or Indigenous are more likely to choose to enter via racial quotas, while students who live with their parents or spouse and students who have some source of income are less likely to participate in the policy.
Vegetation and peat accumulation steer Holocene tidal–fluvial basin filling and overbank sedimentation along the Old Rhine River, The Netherlands
Authors: Pierik Harm Jan , Moree Jelle I. M. , van der Werf Karianne M. , Roelofs Lonneke , Albernaz Marcio Boechat , Wilbers Antoine , van der Valk Bert , van Dinter Marieke , Hoek Wim Z. , de Haas Tjalling , Kleinhans Maarten G. 
Published: September 2022

AbstractIn the transformation from tidal systems to freshwater coastal landscapes, plants act as eco‐engineering species that reduce hydrodynamics and trap sediment, but nature and timing of the mechanisms of land creation along estuaries remains unclear. This article focuses on the Old Rhine estuary (The Netherlands) to show the importance of vegetation in coastal landscape evolution, predominantly regarding tidal basin filling and overbank morphology. This estuary hosted the main outflow channel of the river Rhine between ca 6500 to 2000 cal bp, and was constrained by peat during most of its existence. This study reconstructs its geological evolution, by correlating newly integrated geological data and new field records to varying conditions. Numerical modelling was performed to test the inferred mechanisms. It was found that floodbasin vegetation and resulting organic accumulation strongly accelerated back‐barrier infill, by minimizing tidal influence. After tidal and wave transport had already sufficiently filled the back‐barrier basin, reed rapidly expanded from its edges under brackish conditions, as shown by diatom analysis and datings. Reed growth provided a positive infilling feedback by reducing tidal flow and tidal prism, accelerating basin infilling. New radiocarbon dates show that large‐scale crevassing along the Old Rhine River – driven by tidal backwater effect – only started as nutrient‐rich river water transformed the floodbasin into an Alder carr in a next phase of estuary evolution. Such less dense vegetation promotes crevassing as sediments are more easily transported into the floodbasin. As river discharge increased and estuary mouth infilling progressed, crevasse activity diminished around 3800 to 3000 cal bp, likely due to a reduced tidal backwater effect. The insights from this data‐rich Holocene study showcase the dominant role that vegetation may have in the long‐term evolution of coastal wetlands. It provides clues for effective use of vegetation in vulnerable wetland landscapes to steer sedimentation patterns to strategically adapt to rising water levels.
A conversation with Elja Arjas (Helsinki, November 2021 and March 2022)
Authors: Corander Jukka 
Published: September 2022

AbstractStatistics as an independent scientific discipline is relatively young in Finland. Its active history stretches back roughly a century, with the past 50 years signifying a period of growth. Few other academics such as Elja Arjas, now professor emeritus at University of Helsinki, have played a prominent role in establishing statistics in Finland. This conversation tries to illuminate how this came to happen and what was needed to push statistics as a discipline to a firmer ground. We do not have a looking glass at our disposal but will nevertheless also try make some predictions about the future.
Generic inferential rules for slurs and contrasting senses
Authors: Valtonen Pasi 
Published: September 2022

AbstractThis article offers a new perspective on the relationship between slurring terms and their neutral counterparts with an inferentialist view of slurs. I argue that slurs and their counterparts are coextensional with contrasting senses. Crucially, the proposed inferentialist view overcomes the combination of two challenges: Kaplanian inferences and the substitution argument. The previous views cannot account for both of them.
Inhibition of shoot‐expressed NRT1.1 improves reutilization of apoplastic iron under iron‐deficient conditions
Authors: Ye Jia Yuan , Zhou Miao , Zhu Qing Yang , Zhu Ya Xin , Du Wen Xin , Liu Xing Xing , Jin Chong Wei 
Published: September 2022

SUMMARYIron deficiency is a major constraint for plant growth in calcareous soils. The interplay between NO3− and Fe nutrition affects plant performance under Fe‐deficient conditions. However, how NO3− negatively regulates Fe nutrition at the molecular level in plants remains elusive. Here, we showed that the key nitrate transporter NRT1.1 in Arabidopsis plants, especially in the shoots, was markedly downregulated at post‐translational levels by Fe deficiency. However, loss of NRT1.1 function alleviated Fe deficiency chlorosis, suggesting that downregulation of NRT1.1 by Fe deficiency favors plant tolerance to Fe deficiency. Further analysis showed that although disruption of NRT1.1 did not alter Fe levels in both the shoots and roots, it improved the reutilization of apoplastic Fe in shoots but not in roots. In addition, disruption of NRT1.1 prevented Fe deficiency‐induced apoplastic alkalization in shoots by inhibiting apoplastic H+ depletion via NO3− uptake. In vitro analysis showed that reduced pH facilitates release of cell wall‐bound Fe. Thus, foliar spray with an acidic buffer promoted the reutilization of Fe in the leaf apoplast to enhance plant tolerance to Fe deficiency, while the opposite was true for the foliar spray with a neutral buffer. Thus, downregulation of the shoot‐part function of NRT1.1 prevents apoplastic alkalization to ensure the reutilization of apoplastic Fe under Fe‐deficient conditions. Our findings may provide a basis for elucidating the link between N and Fe nutrition in plants and insight to scrutinize the relevance of shoot‐expressed NRT1.1 to the plant response to stress.
A data‐driven approach to determine daily platelet order quantities at hospitals
Authors: Mirjalili Mahdi , Abouee‐Mehrizi Hossein , Barty Rebecca , Heddle Nancy M. , Sarhangian Vahid 
Published: September 2022

AbstractBackgroundDetermining the required daily number of platelet units in hospitals is a challenging task due to the high uncertainty in daily usage and short shelf life of platelets.Study Design and MethodsWe developed a linear prediction model to guide the daily ordering quantity of platelet units at a hospital that orders the required units from a central supplier. The predictive model relies on historical demand data and other information from the hospital's information system. The ordering strategy is to place an order at the end of each day to bring the platelet inventory to thepredicteddemand for the next day. Unlike typical prediction models, the quality of the predictions is measured with respect to the resulting inventory costs of wastage and shortage. We used data from two hospitals in Hamilton, Ontario from 2015 to 2016 to train our model and evaluated its performance based on the resulting wastage and shortage rates in 2017.ResultsIn 2017, respectively 1915 and 4305 platelet units were transfused at the two hospitals, with daily average (SD) usage of 5.2 (3.7) and 11.8 (4.4). The expiry (estimated shortage) rates were 8.67% (13.86%), and 2.28% (8.48%) at the two hospitals, respectively. Our baseline model would have reduced the expiry (shortage) rates to 2.54% (4.01%) and 0.05% (0.44%) for the two hospitals, respectively.DiscussionGuiding daily ordering decisions for platelets using our proposed model could lead to a significant reduction of wastage and shortage rates at hospitals.
Hemolytic disease of the fetus and newborn caused by anti‐sD antibody in a GP.Mur/Mur Thai mother and review of the prevalence of sD in Thai blood donors
Authors: Lopez Genghis H. , Emthip Morakot , Suwanwootichai Ploymanee , Millard Glenda M. , Wilson Brett , Onpuns Sunisa , Laemsri Kanchana , Chiewsilp Pimol , Flower Robert L. , Hyland Catherine A. , Liew Yew‐Wah 
Published: September 2022

AbstractBackgroundLow‐prevalence antigen sD (MNS23) is encoded by GYPB c.173C > G. Hemolytic disease of the fetus and newborn (HDFN) due to anti‐sD is rare. A mother delivered a newborn whose red blood cells (RBCs) were DAT‐positive and was later diagnosed with HDFN. Serum from the mother was incompatible with the father's RBCs and was used to screen 184 Thai blood donors. This study aimed to investigate the cause of HDFN in a Thai family and determine the prevalence of sD in Thai blood donors.Materials and MethodsThree family members and four blood donors were investigated in the study. Massively Parallel Sequencing (MPS) was used for genotyping. Standard hemagglutination techniques were used in titration studies, phenotyping, and enzyme/chemical studies. Anti‐s, anti‐Mia, anti‐JENU, and anti‐sD reagents were used in serological investigations.ResultsThe mother was GYP*Mur/Mur. The father and the four donors were GYPB*s/sD predicting S − s + sD+. The baby was GYP*Mur/sD and his RBCs were Mia+, s + w with anti‐s (P3BER) and JENU+w. RBCs from two GYPB*sD‐positive blood donors reacted with anti‐sD (Dreyer). Proteolytic enzyme α‐chymotrypsin‐treated sD+ cells did not react with anti‐sD (Wat) produced by the GP.Mur/Mur mother but reacted with the original anti‐sD (Dreyer).DiscussionThis is the first report of HDFN due to anti‐sD in the Asian population. The genotype frequency for GYPB*sD in a selected Thai blood donor population is 2.2% (4/184). Anti‐sD should be considered in mothers with Southeast Asian or East Asian background when antibody identification is unresolved in pregnancies affected by HDFN.
Red blood cell volume, but not platelet or plasma volume is associated with mortality in neonatal ECMO
Authors: Gancar Jessica L. , Shields Molly C. , Wise Linda , Waller Jennifer L. , Stansfield Brian K. 
Published: September 2022

AbstractBackgroundBlood product transfusions are necessary for critically ill neonates on extracorporeal membrane oxygenation (ECMO). Transfusions are administered in response to unstudied arbitrary thresholds and may be associated with adverse outcomes. The objective of this study was to identify relationships between blood product components and mortality in neonates receiving ECMO support for respiratory indications.Study Design and MethodsA retrospective review of neonates receiving ECMO for respiratory indications from 2002 to 2019 from a single quaternary‐referral neonatal intensive care unit (NICU). Demographic and outcome data and transfusion volume (ml/kg/day) were harvested from the medical record, and baseline mortality risk was assessed using NEO‐RESCUERS scores. The association between volume of red blood cells (RBC), platelet, plasma transfusion rates (ml/kg/day), and mortality on ECMO were assessed after adjustment for NEO‐RESCUERS score. Cox proportional hazards (CPH) competing risk model was used to estimate hazard ratios (HR) and 95% confidence intervals (CI) for each variable and mortality outcome.Measurements and Main ResultsAmong 248 neonates undergoing ECMO for respiratory failure, overall survival was 93%. RBC, platelet, and plasma volume were highly associated with mortality during ECMO in an unadjusted model. After adjusting for NEO‐RESCUERS score, RBC volume was associated with increased mortality risk (HR 1.013, 95% CI 1.004–1.022, p = .0043), but platelet and plasma volume were not associated with mortality.ConclusionsRBC, but not platelet or plasma volume, is associated with mortality in neonates on ECMO. Our findings refute previous studies demonstrating an association between platelet volume and mortality for neonates on ECMO.
MARGINALIZATION AND TRANSCENDENCE IN TRANSHUMANISM AND MINJUNG THEOLOGY
Authors: Song Yong Sup , Geraci Robert M. 
Published: September 2022

AbstractThe visibility of transhumanism in pop culture reveals its dramatic advance in twenty‐first‐century life. The more widespread the movement becomes, the more important it is to consider how transhumanism might be made relevant to global humanity. This article orients technological progress by drawing transhumanism into conversation with minjung theology from Korea. Minjung theology offers global tech culture—and its pursuit of technological salvation—an ethical foundation through attention toHan(an emotion specific to those who suffer from individual, sociopolitical, economic, and cultural oppression but have been unable to express it adequately) and the lived reality of those who are often excluded from benefits of technological society. Working in the other direction, transhumanist perspectives on technology offer minjung theology an opportunity to expand its reach through the development of a transcendent theological perspective.
Vivienda social: programa, familias y nuevos modos de habitar. Aportes desde la complejidad del proyecto
Authors: Fiscarelli Diego 
Published: September 2022

Este trabajo problematiza nociones e instrumentos de la praxis proyectual de las operatorias recientes de construcción de vivienda social en Argentina. Se hipotetiza que existe una relación directa entre el alcance del programa arquitectónico y la capacidad funcional de los modelos habitacionales para asimilar las transformaciones sociales recientes en materia de dinámicas del habitar. ¿Cómo han entrado en crisis los patrones tradicionales para caracterizar a los destinatarios, y qué dimensiones debería interpelar desde las significaciones imaginarias, la construcción programática de la vivienda social en la actualidad?
Desde los recientes avances en relación con un campo epistemológico específico para la producción de conocimiento disciplinar —el Programa Complejo para la Investigación Proyectual— y articulando voces especializadas en familias, cambios sistémicos y nuevos modos de habitar, se propone una discusión bibliográfica orientada a identificar los parámetros aún vigentes que determinan límites en la relación programa-proyecto.
Particle Image Velocimetry in a High-Speed Short-Duration Turbine Rig
Authors: Okada Mizuki , Pinho Jorge , Cernat Bogdan , Lavagnoli Sergio 
Published: September 2022

AbstractThis article presents the implementation of a particle image velocimetry (PIV) into the high-speed short-duration rotating turbine facility of the von Karman Institute. The advantage of PIV as a whole-field measurement is emphasized in such circumstances for which the use of optical technique can drastically reduce the number of tests and the need for multiple intrusive expensive probes, ultimately lowering the testing cost. Practical solutions were demonstrated that address various challenges for the effective application of PIV. An endoscope delivered the laser sheet to the region of interest and a plano-concave window provided optical access for the measurement in the annular test section. Sub-micron scale oil droplets were seeded into the main flow through custom-made probes located upstream of the nozzle guide vane and into a pipeline supplying rim seal purge flow. A high-speed laser system and a high-speed camera were synchronized at 1 kHz sampling rate. Complementary measurements and dedicated image processing were performed to ensure the synchronization of the PIV images with the rotor position that was monitored through an encoder. The region of interest was the blade-to-blade plane at the 58% span turbine exit on a rectangular field of view covering approximately one rotor pitch and 0.15 rotor axial chord from the rotor trailing edge. Phase-locked-average velocity fields are obtained from PIV and compared against steady-state Reynolds-averaged Navier–Stokes (RANS) simulations along with four-hole probe measurement results. Together with uncertainty analysis, the results demonstrate the promising robustness and accuracy of PIV. A practical guideline for PIV application in such kinds of turbine test rigs is provided as a conclusion of the paper.