bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA PVT-1 promotes osteosarcoma cancer stem-like properties through direct interaction with TRIM28 and TSC2 ubiquitination
Authors: Yustein Jason , Tsang Susan , Rainusso Nino , Nomura Motonari , Patel Tajhal , Nakahata Kengo , Kim Ha Ram , Huang Shixia , Rajapakshe Kimal , Coarfa Cristian , Man Tsz-Kwong , Rao Pulivarthi , Liu Meng 
Published: July 2022

Abstract
Osteosarcoma, the most common pediatric bone tumor, is an aggressive heterogeneous malignancy defined by complex chromosomal aberrations. Overall survival rates remain at approximately 70%, but patients with chemoresistant or metastatic disease have extremely poor outcomes of less than 30%. A subgroup of tumors harbor amplification of chromosome 8q24.2 and increased expression of the oncogenic long noncoding RNA (lncRNA) Plasmacytoma Variant Translocation-1 (PVT-1), which is associated with an extremely poor clinical prognosis. Through in vitro assays, this study demonstrated that PVT-1 is critical for osteosarcoma tumor-initiation potential. Chromatin Hybridization by RNA Purification analysis identified Tripartite-Motif Containing Family 28 (TRIM28) as a novel PVT-1 binding partner. Mechanistically, co-immunoprecipitation studies showed the PVT-1/TRIM28 complex binds and increases SUMOylation of phosphatidylinositol 3-kinase catalytic subunit type 3 (Vps34), which leads to enhanced ubiquitination and degradation of tumor suppressor complex 2 (TSC2), thus contributing to stem cell phenotypes. Importantly, osteosarcoma cells with increased PVT-1 have enhanced sensitivity to a SUMOylation inhibitor, TAK-981. Altogether, this study elucidated a role for PVT-1 in the enhancement of cancer stem-like behaviors, including migration and invasion, in osteosarcoma, and identified the novel PVT-1/TRIM28 axis signaling cascade as a potential therapeutic target for osteosarcoma treatment.
A preliminary study to explain the outstanding performance of Streptomyces pactum (Act12) in enhancing phytoextraction of Cd and Zn contaminated soils
Authors: Wang Nina , Ren Jie , Wang Linlin , Wang Yuheng , Wang Ze , Guo Di 
Published: July 2022

Abstract
Streptomyces pactum (Act12) can both promote plant growth and strengthen heavy metal mobilization. Nevertheless, the mechanisms of how Act12 works during the phytoextraction process are still unknown. The present work investigated whether the metabolites produced by Act12 could influence the seed germination and the growth of potherb mustard, and explored its mobilizing effect on soil cadmium (Cd) and zinc (Zn). The results showed that the germination potential and rate of potherb mustard seed treated with Act12 fermentation broth were 1.0 and 0.32 folds higher than those of control, probably due to the interruption of seed dormant stage. We also found that Act12 inoculation not only promoted the dry biomass (6.82%) of potherb mustard, but also increased the leaf chlorophyll (11.8%) and soluble protein (0.35%) production. The boosted seed germination rate under Act12 treatment (up to 63.3%) indicated that Act12 enhanced the resistance of potherb mustard seeds to Cd and Zn and alleviated their physiological toxicity. The generated metabolites during the Act12 fermentation posed positive impact on the availability of soil Cd and Zn. These findings bring new insight into the Act12 assisted phytoextraction of Cd and Zn from contaminated soils.
Humanitarian responses to global emergencies:
The context of Ukrainian war refugees in Poland
Authors: Kossowska Małgorzata , Szwed Paulina , Szumowska Ewa , Perek-Białas Jolanta , Czernatowicz-Kukuczka Aneta 
Published: July 2022

Abstract
The paper investigates the psychological factors associated with the unprecedented assistance that Poles have offered refugees from Ukraine since the outset of Russia’s invasion of Ukraine. Building on social identity theory, and examining the current social context in Poland, we focus on three social identity dimensions, i.e., a feeling of closeness towards refugees from Ukraine, anticipatory fears (of a Russian invasion), and a community norm of helping. These three dimensions predict collective helping resulting from a sense of a common fate and a feeling of togetherness with Ukrainians. We tested this hypothesis in a study (N = 1066) conducted between 11 and 17 March 2022. Participants were asked about their helping activities during the previous week; they also responded to questions on different measures of social identity processes. The results support our expectations, revealing that closeness, anticipatory fears, and social norms are associated with two forms of help: benevolent and activist. The results of the study contribute to the discussion on social identity processes underlying offers of help to people fleeing from war-zones. Thus, they enhance our understanding of the role of citizens in terms of their contribution to helping refugees, and can be used to improve responses to other humanitarian crises.
Abnormal cerebral blood flow in patients with Leber's hereditary optic neuropathy.
Authors: Wang Ling , Ji Yi , Ding Hao , Tian Qin , Fan Ke , Shi Dapeng , Yu Chunshui , Qin Wen 
Published: July 2022

Abstract
Purpose
The study aimed to unravel abnormal cerebral blood flow (CBF) in patients with Leber’s hereditary optic neuropathy (LHON) using arterial spin labeling (ASL) and to investigate the associations among disrupted CBF, disease duration, and neuro-ophthalmological impairment.
Methods
ASL perfusion imaging data was collected from 20 patients with acute LHON, 29 patients with chronic LHON, and 37 healthy controls. We used a one-way analysis of covariance to test the intergroup differences in CBF. Linear and nonlinear curve fit models were applied to explore the associations among CBF, disease duration, and neuro-ophthalmological metrics.
Results
Brain regions differed in LHON patients included the left sensorimotor and bilateral visual areas (p < 0.05, cluster-wise family-wise error correction). Acute and chronic LHON patients demonstrated lower CBF in bilateral calcarine than the healthy controls. Chronic LHON had lower CBF in the left middle frontal gyrus and sensorimotor cortex, and temporal-partial junction than the healthy controls and acute LHON. A significant logarithmic negative correlation was shown between CBF of left middle frontal gyrus and disease duration. There showed a significant logarithmic positive correlation between retinal nerve fiber layer thickness and CBF in left middle frontal gyrus, and negative correlations between loss of variance and CBF in left middle frontal gyrus and sensorimotor cortex (p < 0.05, Bonferroni correction).
Conclusion
LHON patients exhibited reduced CBF in the visual pathway, sensorimotor and higher-tier cognitive areas. Disease duration and neuro-ophthalmological impairments can influence the metabolism of non-visual areas.
Phenotypic Variation and Genotypic Diversity of Finger Millet (Eleusine coracana L. Gaertn) Germplasm Collections in Malawi.
Authors: Yalaukani Louis , Moses Maliro , Michael Chipeta , Davis Gimode , Vicki Morrone , Abel Sefasi , Lawrent Pungulani , Daimon Kambewa , Fatsani Keffa , Frank Tchuwa , Peter Soko , Kapyira Wilson , Nachanje Peterson 
Published: July 2022

Abstract
Finger millet (Eleusine coracana L. Gaertn) has remained neglected and underutilized for years in Malawi partly due to limited research that include crop improvement. Any effort to exploit the genetic potential of the available genetic variability would lead to identification of potential germplasm for production in Malawi. The study aimed at characterizing agro-morphological traits and identifying genetic diversity using Kompetitive Allele Specifc PCR (KASP) single nucleotide polymorphism (SNP) markers in order to identify unique traits that can be used in germplasm discrimination for seed production as well as crop improvement. A total of 40 finger millet germplasm collections were evaluated in the field at Crops and Soil Sciences Department's research farm of Lilongwe University of Agriculture and Natural Resources (LUANAR). The experiment was laid out in an Alpha lattice design. From 40 germplasm collections, 188 germplasm collection samples were selected for Deoxyribonucleic Acid (DNA) extraction. Phenotypic data on agro-morphological traits was collected and subjected to multivariate analysis. Genotypic data was subjected to Analysis of Molecular Variance (AMOVA) in R- statistical package. Three principal components explained 42% of the total phenotypic variation among the germplasm collections while AMOVA revealed a 48% variation within and 53% variation among the germplasm collections. Cluster analysis grouped the 188 genotyped germplasm collections into 6 clusters. Phenotypic variations and genotypic diversity exist in finger millet which aids in the discrimination of the germplasm collections in seed production and in breeding programs.
Inactivation of SARS-CoV-2 on Salt-Coated Surfaces: An In Vitro Study
Authors: Gsell Monika , Bulliard Xavier , Weber Sandra Schorderet , Xiang Yang , Constant Samuel , Steiner Sandro , Biselli Silvia , Pugin Raphael , Palmieri Michele , Hogg Andreas , Peitsch Manuel C. , Hoeng Julia , Stan Adrian 
Published: July 2022

Abstract
In the Covid-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), face masks have become a very important safety measure against the main route of transmission of the virus: droplets and aerosols. Concerns that masks contaminated with SARS-CoV-2 infectious particles could be a risk for self-contamination have emerged early in the pandemic as well as solutions to mitigate this risk. The coating of masks with sodium chloride, an anti-viral and non-hazardous to health chemical, could be an option for reusable masks. To assess the antiviral properties of salt coatings deposited onto common fabrics by spraying and dipping, the present study established an in vitro bioassay using three-dimensional airway epithelial cell cultures and SARS-CoV-2 virus. Virus particles were given directly on salt-coated material, collected, and added to the cell cultures. Infectious virus particles were measured by plaque forming unit assay and in parallel viral genome copies were quantified over time. Relative to noncoated material, the sodium chloride coating significantly reduced virus replication, confirming the effectiveness of the method to prevent fomite contamination with SARS-CoV-2. In addition, the lung epithelia bioassay proved to be suitable for future evaluation of novel antiviral coatings.
Strategies for de-implementation of low-value care – a scoping review
Authors: Ingvarsson Sara , Hasson Henna , Schwarz Ulrica von Thiele , Nilsen Per , Powell Byron J. , Lindberg Clara , Augustsson Hanna 
Published: July 2022

Abstract
Background
The use of low-value care (LVC) is a persistent problem that calls for knowledge about strategies for de-implementation. However, studies are dispersed across many clinical fields, and there is no overview of strategies that can be used to support the de-implementation of LVC. The extent to which strategies used for implementation are also used in de-implementing LVC is unknown. The aim of this scoping review is to (1) identify strategies for the de-implementation of LVC described in the scientific literature and (2) compare de-implementation strategies to implementation strategies as specified in the Expert Recommendation for Implementing Change (ERIC).
Method
A scoping review was conducted according to recommendations outlined by Arksey and O’Malley. Four scientific databases were searched, relevant articles were snowball searched, and the journal Implementation Science was searched manually for peer-reviewed journal articles in English. Articles were included if they were empirical studies of strategies designed to reduce the use of LVC. Two reviewers conducted all abstract and full-text reviews, and conflicting decisions were discussed until consensus was reached. Data were charted using a piloted data-charting form. The strategies were first coded inductively and then mapped onto the ERIC compilation of implementation strategies.
Results
The scoping review identified a total of 71 unique de-implementation strategies described in the literature. Of these, 62 strategies could be mapped onto ERIC strategies, and four strategies onto one added category. Half (50%) of the 73 ERIC implementation strategies were used for de-implementation purposes. Five identified de-implementation strategies could not be mapped onto any of the existing strategies in ERIC.
Conclusions
Similar strategies are used for de-implementation and implementation. However, only a half of the implementation strategies included in the ERIC compilation were represented in the de-implementation studies, which may imply that some strategies are being underused or that they are not applicable for de-implementation purposes. The strategies assess and redesign workflow (a strategy previously suggested to be added to ERIC), accountability tool, and communication tool (unique new strategies for de-implementation) could complement the existing ERIC compilation when used for de-implementation purposes.
Comparative study of the rock-breaking mechanism of a disc cutter and wedge tooth cutter in Qingdao Andesite Strata
Authors: Jiang Hua , Zhao Huiyan , Zhang Xiaoyan , Jiang Yusheng , Zheng Yaofu 
Published: July 2022

Abstract
The operation of a shield tunnel boring machine (TBM) in a high-strength hard rock stratum results in significant cutter wear, adversely affecting the thrust and torque of the cutter head. The rock-breaking performance of cutters in a slightly weathered andesite stratum in the Qingdao Metro Line 8 tunnel was investigated using the discrete element method and theoretical analysis to determine the stress characteristics and optimize the cutter parameters. The rock-breaking processes of a disc cutter and wedge tooth cutter were simulated by PFC3D numerical simulation software, and the rock-breaking degree, stress of the cutter, and rock-breaking specific energy were analyzed. The rock damage caused by the cutter in a specific section was divided into three stages: the advanced influence, crushing, and destabilizing stages. The rock-breaking degree and the tangential and normal forces of the wedge tooth cutter are larger than that of the disc cutter under the same conditions. The disc cutter (wedge tooth cutter) has the highest rock-breaking efficiency at a cutter spacing of 100 mm (110 mm) and a penetration depth of 8 mm (10 mm), and the rock-breaking specific energy is 11.48 MJ/m3 (12.05 MJ/m3). Therefore, two types of cutters with different penetration depths or cutter spacings should be considered. The number of teeth of wedge tooth cutters can be increased in hard strata to improve the rock-breaking efficiency of the shield. The research results provide a reference for shield cutterhead selection and cutter layout in similar projects.
Root-inhabiting bacterial microbiota of the perennial sweet sorghum cultivar at the maturing stage under cold stress
Authors: Lu Gui-Hua , Cao Rui , Fazal Aliya , Zheng Kezhi , Na Zhiye , Yang Yonghua , Sun Bo , Yang Hongjun , Na Zhong-Yuan 
Published: July 2022

Abstract
The bacterial microbiota inhabiting the endosphere and rhizoplane play numerous important functions in improving plant growth. The mutualistic interaction between sweet sorghum and soil bacteria has drawn increasing attention. However, the bacterial microbiota inhabiting the roots of sweet sorghum’s perennial analog have rarely been characterized. Here, the root-inhabiting bacterial microbiota of the perennial sweet sorghum cultivar NaPBS778 (N778 simply) and its control TP60 were discovered at the flowering and maturing stages under field growth by high-throughput amplicon sequencing of the 16S rRNA gene via Illumina MiSeq. Almost all alpha diversity indices of aerial and primary root samples of N778 were not significantly different from those of TP60 at the maturing stage, except for the observed species (Sobs) and phylogenetic diversity indices. Next, the beta diversity of aerial and primary root samples showed no significant differences between N778 and its control TP60 at the maturing stage. Moreover, the bacterial microbiota in N778 aerial and primary roots was not only predominated by Proteobacteria, Actinobacteria, and Bacteroidetes at the phylum level but was also strikingly distinct from the bacterial microbiota in rhizosphere soil at the genus level. Additionally, the root samples of N778 at the maturing stage were considerably enriched with OTU1262, OTU434, OTU1304, and OTU836, which belonged to the genera Pseudarthrobacter, Streptomyces, and an unclassified genus of the family Oxalobacteraceae, respectively. Our findings imply that the perennial sweet sorghum cultivar N778 may recruit not only potentially cold-tolerant but also plant growth-promoting and nitrogen stress-tolerant bacterial taxa into roots at the maturing stage.
Default mode network mechanisms of repeated transcranial magnetic stimulation in heroin addiction
Authors: Jin Long , Yuan Menghui , Zhang Wei , Wang Lei , Chen Jiajie , Wang Fan , Zhu Jia , Liu Tao , Wei Yixin , Li Yunbo , Wang Wei , Li Qiang , Wei Longxiao 
Published: July 2022

Abstract
Repetitive transcranial magnetic stimulation (rTMS) over the left dorsolateral prefrontal cortex (DLPFC) has been found could reduce the craving of heroin dependent (HD) individuals, but the underlying mechanisms of the anti-craving effect of rTMS is unknown. It has been shown that abnormalities in the default mode network (DMN) are consistent findings in HD individuals, in which the craving was involved. We assessed the effect of rTMS on the activity of the DMN and the relation to treatment response. Thirty HD individuals were included in this self-controlled study. All HD participants received a 7-times 10Hz rTMS during a week. The craving, withdrawal symptom and resting-state functional magnetic resonance imaging data were collected before and after the rTMS treatment. Thirty demographically matched healthy subjects who did not receive rTMS were included as controls. We focused on the change of coupling seeded from the medial prefrontal cortex (MPFC), posterior cingulate cortex (PCC) and bilateral inferior parietal lobe (IPL) which are core regions of the DMN. The craving and withdrawal symptom score of the HD individuals significantly decreased after rTMS treatment. The left IPL-left middle frontal gyrus coupling and the left IPL-right inferior occipital gyrus coupling significantly decreased, and the change of the left IPL-left middle frontal gyrus coupling positively correlated with the change of the drug-cue induced craving. The rTMS could modulate the coupling between the DMN and ECN. The alteration of the left IPL-left middle frontal gyrus coupling may play an important mechanistic role in reducing the drug-cue induced craving.
Bandpass and bandstop plasmonic filters based on Wilkinson power divider structure
Authors: Korani Nastaran , Danaie Mohammad , Abbasi Abdollah 
Published: July 2022

Abstract
The plasmonic bandpass and bandstop filter based on the Wilkinson power divider structure with the nanoring resonators are proposed and demonstrated. we find that the resonance wavelengths can be manipulated by adjusting the inner radius and outer radius of the nanoring resonator. Using the manipulation of the gap size between the ring resonators and rectangular stubs the bandpass and bandstop filters were designed. When the angle between the input waveguide and ring resonators is changed, the first mode is suppressed. The propagating modes of surface plasmon polaritons (SPPs) are investigated in this filter. The finite-difference time-domain (FDTD) method is utilized to investigate and analyze the transmission characteristics of the proposed filter. The proposed plasmonic filter is of a compact size that has potential application for integrated optics. The proposed technique can suppress the modes. The results show that the filtering wavelength can be manipulated by changing the stubs and rings parameters.
Mapping and characterization of a novel adult-plant leaf rust resistance gene Lr16G216 via BSA and conventional linkage method
Authors: Zhao Renhui , Liu Bingliang , Wan Wentao , Jiang Zhengning , Chen Tiantian , Wang Ling , Shou Lulu , Bie Tongde 
Published: July 2022

Abstract
Leaf rust (Puccinia triticina) is one of the most devastating fungal diseases of wheat (Triticum aestivum L.). Discovery and identification of new resistance genes is essential to develop disease-resistant cultivars. An advanced breeding line Yang16G216 was previously identified to confer adult plant resistance (APR) to leaf rust. In this research, a recombinant inbred line (RIL) population was constructed from the cross between Yang16G216 and a highly susceptible line Yang16M6393, and genotyped with exome capture sequencing and 55K SNP array. Through bulked-segregant analysis (BSA) and genetic linkage mapping, a stable APR gene, designated as Lr16G216, was primarily detected and mapped to the distal region of chromosome arm 6BL with a genetic interval of 2.8-cM. For further verification, another RIL population derived from the cross between Yang16G216 and a susceptible wheat variety Yangmai 29 were analyzed using the enriched markers in the target interval, and Lr16G216 was further narrowed to a 0.59-cM genetic interval flanked by the KASP markers Ax109403980 and Ax95083494, corresponding to the physical position 712.34–713.94 Mb in the Chinese Spring reference genome, in which twenty-six disease resistance-related genes were annotated. Based on leaf rust resistance spectrum, mapping data and physical location, Lr16G216 is identified to be a novel and effective APR gene. The Lr16G216 with linked markers will be useful for marker-assisted selection in wheat resistance breeding.
Moderators of Physical Activity and Quality of Life Response to a Physical Activity Intervention for Breast Cancer Survivors
Authors: Schleicher Erica , McAuley Edward , Courneya Kerry S , Anton Phillip , Ehlers Diane K. , Phillips Siobhan M. , Oster Robert A. , Pekmezi Dorothy , Rogers Laura Q 
Published: July 2022

Abstract
Purpose
Moderate-to-vigorous physical activity (MVPA) can improve quality of life (QoL) for breast cancer survivors (BCS); yet most do not achieve 150 + weekly minutes of MVPA. This study investigated moderators of response to a physical activity (PA) behavior change intervention for BCS.
Methods
BCS (N = 222) were randomized to the 3-month intervention (BEAT Cancer) or usual care. Measurements occurred at baseline, post-intervention, and 3 months post-intervention. Measures included accelerometry, self-reported MVPA, and Functional Assessment of Cancer Therapy (FACT-General, FACT-Breast, physical well-being [PWB], social well-being [SWB], emotional well-being [EWB], functional well-being [FWB], additional concerns [AC], and Trial Outcome Index (TOI).
Results
Adjusted linear mixed-model analyses indicated individuals ≤ 24 months post-diagnosis who were single reported smaller increases in weekly MVPA than those > 24 months (44.07 vs 111.93) and partnered (-16.24 vs. 49.16 min), all p’s < 0.05. As for QoL, participants < 12 months post-diagnosis who received chemotherapy experienced smaller improvements than those ≥ 12 months in FACT-General, FACT-Breast, PWB, SWB scores. Survivors with a history of chemotherapy had smaller improvements in FACT-General, FACT-Breast, PWB, SWB, TOI, and AC scores, all p’s < 0.05.
Conclusion
These findings indicate that being < 2 years post-diagnosis, single, and having history of chemotherapy may limit MVPA and QOL responses to a physical activity intervention. Further studies are needed to determine if and/or what additional PA supports and resources these subgroups of BCS might find beneficial and effective.
Trial Registration:
ClinicalTrials.gov number: NCT00929617