bluuebunny/crossref_metadata_2025_split huggingface dataset:
Auswirkungen eines erhöhten Wassergehalts auf lösliche Staubkuchenbestandteile in der Oberflächenfiltration – Teil 2: Experimentelle Untersuchungen
Authors: Schwarz Almuth D. , Horst David , Meyer Jörg , Zhang Qian , Dittler Achim , Schmidt Eberhard 
Published: April 2022

AbstractEs wird ein erhöhter Wassergehalt im Trägergas in der Oberflächenfiltration und dessen Einfluss auf lösliche und unlösliche Partikeln im Filterkuchen untersucht. Der erste Teil beschrieb die gemeinsamen Grundlagen und den Stand des Wissens. Die Beeinflussung von Haftkräften und der mögliche Stofftransport von Partikelmaterial in gelöster und ungelöster Form wurden als Auswirkungen eines erhöhten Wassergehalts diskutiert. Der zweite Teil behandelt eine Reihe von experimentellen Untersuchungen und schildert hierfür das Vorgehen und exemplarische Ergebnisse für unterschiedliche Wasserbeaufschlagung im Hinblick auf Menge und Aggregatzustand, erhöhte relative Feuchte zum einen und flüssige Wassertropfen zum anderen.
Length‐Dependent Collective Vibrational Dynamics in Alpha‐Helices
Authors: Zhang Zhenyu , Chen Mu , Zhan Lijian , Zheng Fei , Si Wei , Sha Jingjie , Chen Yunfei 
Published: April 2022

AbstractFunctions of protein molecules in nature are closely associated with their well‐defined three‐dimensional structures and dynamics in body fluid. So far, many efforts have been made to reveal the relation of protein structure, dynamics, and function, but the structural origin of protein dynamics, especially for secondary structures as building blocks of conformation transition, is still ambiguous. Here we theoretically uncover the collective vibrations of elastic poly‐alanine α‐helices and find vibration patterns that are distinctively different over residue numbers ranging from 20 to 80. Contrary to the decreasing vibration magnitude from ends to the middle region for short helices, the vibration magnitude for long helices takes the minimum at approximately 1/5 of helix length from ends but reaches a peak at the center. Further analysis indicates that major vibrational modes of helical structures strongly depend on their lengths, where the twist mode dominates in the vibrations of short helices while the bend mode dominates the long ones analogous to an elastic Euler beam. The helix‐coil transition pathway is also affected by the alternation of the first‐order mode in helices with different lengths. The dynamic properties of the helical polypeptides are promising to be harnessed for de novo design of protein‐based materials and artificial biomolecules in clinical treatments.
Competition between Intra and Intermolecular Pnicogen Bonds: Complexes between Naphthalene Derivatives and Neutral or Anionic Bases
Authors: Wysokiński Rafał , Zierkiewicz Wiktor , Michalczyk Mariusz , Scheiner Steve 
Published: April 2022

AbstractThe PnF2(Pn=P,As,Sb,Bi) on a naphthalene scaffold can engage in an internal pnicogen Pn⋅⋅⋅N bond (PnB) with an NH2group placed close to it on the adjoining ring. An approaching neutral NH3molecule can engage in a second PnB with the central Pn, which tends to weaken the intramolecular bond. The presence of the latter in turn weakens the intermolecular PnB with respect to that formed in its absence. Replacement of the external NH3by a CN−anion causes a fundamental change in the bonding pattern, producing a fourth covalent bond with Pn, which rearranges into a trigonal bipyramidal motif. This addition disrupts the internal Pn⋅⋅⋅N pnicogen bond, recasting the PnF2⋅⋅⋅NH2interaction into an NH⋅⋅⋅F H‐bond.
The small and medium enterprises' green human resource management and green transformational leadership: A sustainable moderated‐mediation practice
Authors: Chen Yu‐Shan , Yan Xin 
Published: April 2022

AbstractThis study explores how green human resource management (GHRM) and green transformational leadership (GTL) affect green organizational pride (GOP). We conduct a survey study of moderated‐mediation sustainable practice in small and medium enterprises (SMEs) in “Hokkien Golden Triangle” of China based on the combination of resource‐based view, social exchange theory, positive psychology, and social cognitive theory. Especially, we develop two new concepts, “green organizational pride” and “green psychological capital” (GPC) to verify that the positive relationships between GHRM/GTL and GOP are mediated by GPC. Besides, we expand the research of environmental culture (EC) by further predicting that it could amplify the indirect effects of GHRM/GTL on GOP via GPC. The findings contribute to sustainability literature by highlighting the importance of GHRM and GTL in Chinese sustainable practice. Moreover, the results can advance the prior literature in the area of sustainability and create a new green path for developing countries.
Precision and bias of spatial capture–recapture estimates: A multi‐site, multi‐year Utah black bear case study
Authors: Schmidt Greta M. , Graves Tabitha A. , Pederson Jordan C. , Carroll Sarah L. 
Published: April 2022

AbstractSpatial capture–recapture (SCR) models are powerful analytical tools that have become the standard for estimating abundance and density of wild animal populations. When sampling populations to implement SCR, the number of unique individuals detected, total recaptures, and unique spatial relocations can be highly variable. These sample sizes influence the precision and accuracy of model parameter estimates. Testing the performance of SCR models with sparse empirical data sets typical of low‐density, wide‐ranging species can inform the threshold at which a more integrated modeling approach with additional data sources or additional years of monitoring may be required to achieve reliable, precise parameter estimates. Using a multi‐site, multi‐year Utah black bear (Ursus americanus) capture–recapture data set, we evaluated factors influencing the uncertainty of SCR structural parameter estimates, specifically density, detection, and the spatial scale parameter, sigma. We also provided some of the first SCR density estimates for Utah black bear populations, which ranged from 3.85 to 74.33 bears/100 km2. Increasing total detections decreased the uncertainty of density estimates, whereas an increasing number of total recaptures and individuals with recaptures decreased the uncertainty of detection and sigma estimates, respectively. In most cases, multiple years of data were required for precise density estimates (<0.2 coefficient of variation [CV]). Across study areas there was an average decline in CV of 0.07 with the addition of another year of data. One sampled population with very high estimated bear density had an atypically low number of spatial recaptures relative to total recaptures, apparently inflating density estimates. A complementary simulation study used to assess estimate bias suggested that when <30% of recaptured individuals were spatially recaptured, density estimates were unreliable and ranged widely, in some cases to >3 times the simulated density. Additional research could evaluate these requirements for other density scenarios. Large numbers of individuals detected, numbers of spatial recaptures, and precision alone may not be sufficient indicators of parameter estimate reliability. We provide an evaluation of simple summary statistics of capture–recapture data sets that can provide an early signal of the need to alter sampling design or collect auxiliary data before model implementation to improve estimate precision and accuracy.
Prudent Choice of Iron‐Based Metal‐Organic Networks for Solvent‐Free CO2 Fixation at Ambient Pressure
Authors: Mitra Antarip , Biswas Tanmoy , Ghosh Sourav , Tudu Gouri , Paliwal Khushboo S. , Ganatra Pragati , Mahalingam Venkataramanan 
Published: April 2022

AbstractThe rising global warming and associated climate change demand efficient tactics to reduce CO2 concentration in the atmosphere. Conversion of epoxides to cyclic carbonates utilizing CO2 is a promising and sustainable approach towards CO2 fixation. However, the inert nature of CO2 and high activation energy requirement for the particular reaction necessitate the use of efficient catalysts. In this work, we have designed and developed a heterogeneous catalyst using catechol functionalized guanidinium based ligands (L1) and Fe(III) ions to perform the CO2 fixation reaction. The resulting amorphous material (Fe−L1) possesses metal‐organic network as revealed through different characterization methods. The optimized catalyst Fe−L1 is efficient for the conversion of a variety of epoxides into their corresponding cyclic carbonates in very good yield (>80 %). The reactions were performed under atmospheric pressure without solvent and external additives (e. g., TBAI or KI). The investigation of the mechanistic pathway indeed validates the synergistic effect of metal and halide ions that leads to the efficient conversion of different epoxides into cyclic carbonates. Furthermore, no significant loss in the catalytic activity of the Fe−L1 is noticed up to six cycles. The post catalytic analyses clearly indicate the robust nature of the catalyst. The developed one component bifunctional catalytic system can pave way towards transition to sustainable carbon capture and conversion.
Highly Efficient and Stereoselective Synthesis of 6,7‐Dideoxy‐β‐d‐ido‐octopyranuronates
Authors: Homayonia Saba , Ling Chang‐Chun 
Published: April 2022

Abstract6‐Deoxy‐β‐ido‐heptopyranosides are challenging glycosides to synthesize due to their unusual 6‐deoxy‐heptose backbone, rare ido‐configuration and most importantly, the β‐1,2‐cis anomeric linkage. They are found in nature, such as being part of the repeating disaccharide of C. jejuni HS:4 capsular polysaccharides (CPs). Interestingly, the bacterial CPs is found to be partially modified with an O‐methyl phosphoramidate (MeOPN) functionality at the O‐2 or O‐7 position of the 6‐deoxy‐β‐d‐ido‐heptopyranosides. In this work, we report the first synthesis of three analogous of β‐d‐ido‐octopyranosides (1–3) that contain a 6,7‐dideoxy‐functionality and either a terminal methyl ester or carboxylic acid or amide. Since carboxylic acids have been reported as bioisosteres of phosphate group, compound 1–3 can be regarded as carbon‐based bioisosteres of 6‐Deoxy‐β‐ido‐heptopyranoside containing the MeOPN group without a chiral center. Compounds (1–3) were efficiently synthesized from a O‐2 activated β‐d‐galacto‐octopyranuronate (19) that was efficiently converted to the desired β‐d‐ido‐octopyranuronate configuration using an elegant one‐pot process.
Linear mixed models to handle missing at random data in trial‐based economic evaluations
Authors: Gabrio Andrea , Plumpton Catrin , Banerjee Sube , Leurent Baptiste 
Published: April 2022

AbstractTrial‐based cost‐effectiveness analyses (CEAs) are an important source of evidence in the assessment of health interventions. In these studies, cost and effectiveness outcomes are commonly measured at multiple time points, but some observations may be missing. Restricting the analysis to the participants with complete data can lead to biased and inefficient estimates. Methods, such as multiple imputation, have been recommended as they make better use of the data available and are valid under less restrictive Missing At Random (MAR) assumption. Linear mixed effects models (LMMs) offer a simple alternative to handle missing data under MAR without requiring imputations, and have not been very well explored in the CEA context. In this manuscript, we aim to familiarize readers with LMMs and demonstrate their implementation in CEA. We illustrate the approach on a randomized trial of antidepressants, and provide the implementation code in R and Stata. We hope that the more familiar statistical framework associated with LMMs, compared to other missing data approaches, will encourage their implementation and move practitioners away from inadequate methods.
A novel clinical immune‐related prognostic model predicts the overall survival of mantle cell lymphoma
Authors: Lv Huijuan , Fei Yue , Li Wei , Wang Yi , Wang Jinni , He Jin , Liu Xianming , Li Lanfang , Qiu Lihua , Qian Zhengzi , Zhou Shiyong , Meng Bin , Zhai Qiongli , Ren Xiubao , Zou Dehui , Cai Qingqing , Wang Xianhuo , Zhang Huilai 
Published: April 2022

AbstractThe mantle cell lymphoma (MCL) International Prognostic Index (MIPI) and combined MIPI (MIPI‐c) are commonly used for risk classification of MCL patients. However, these indexes lack immune‐related parameters. The purpose of this study was to develop a novel prognostic model that integrated clinical and immune parameters. A total of 189 patients with newly diagnosed MCL from January 2010 to June 2020 were enrolled in our study. A nomogram and immune‐related prognostic index (IRPI) were established to predict the overall survival (OS) of patients according to univariate and multivariate analyses. Discrimination and calibration were used to compare the prognostic performance of the IRPI, MIPI, and MIPI‐c. External validation was performed based on validation dataset (n = 150) from two other centers. The results for the training dataset indicated that B symptoms, platelet count, B2M level, CD4+ T‐cell count<26.7% and CD8+ T‐cell count>44.2% were predictors for OS. All the prognostic factors were integrated into the nomogram. For the overlap of confidence intervals of each variable, we assigned one point for each factor. The IRPI categorized patients into three risk categories: a score of zero indicated low risk, a score of one or two indicated intermediate risk, and a score of ≥3 indicated high risk. The IRPI showed better discrimination and calibration power than the MIPI and MIPI‐c in the training dataset and validation dataset. The novel IRPI is a refined risk stratification index and reflects the strong complementary prognostic effects between clinical and immune parameters in MCL.
Using dissolved organic matter fluorescence to predict total mercury and methylmercury in forested headwater streams, Sleepers River, VermontUSA
Authors: Shanley James B. , Taylor Vivien F. , Ryan Kevin A. , Chalmers Ann T. , Perdrial Julia , Stubbins Aron 
Published: April 2022

AbstractAqueous transport of mercury (Hg) across the landscape is closely linked to dissolved organic matter (DOM). Both quantity and quality of DOM affect Hg mobility, as well as the formation and transport of toxic methylmercury (MeHg), but only a limited number of field studies have investigated Hg and MeHg with respect to specific DOM components. We investigated these interactions at the 41‐ha forested W‐9 catchment at Sleepers River, Vermont, which has a long history of mercury and other biogeochemical research. We examined spatial and temporal patterns of filtered Hg fractions and dissolved organic carbon (DOC) concentration, DOM quality, and major solutes at 12 stream sites within W‐9 and the downstream W‐3 gage (837 ha) over five sampling campaigns including a large (79 mm) fall storm, spring snowmelt, and three seasonally contrasting base flow periods. Filtered total Hg (THg), MeHg, and DOC concentrations increased in order base flow < snowmelt < fall storm, except that MeHg remained at baseflow levels during snowmelt. Ranges of median concentrations across sites for the five campaigns were THg, <0.2–4.1 ng L−1; MeHg, <0.03–0.45 ng L−1; and DOC, 0.8–14.0 mg L−1. Humic‐like DOM fluorescence components, as determined by parallel factor analysis (PARAFAC), dominated the fluorescence across sites and sampling campaigns. THg correlated strongly (r > 0.94) with these humic components, but even more strongly with bulk DOC and absorbance at 254 nm (UV254;r > 0.96), and less strongly with protein‐like DOM (0.7 < r < 0.9). MeHg correlated in the same order but less strongly with humic‐ (0.8 < r < 0.9) and protein‐like (0.6 < r < 0.8) DOM. MeHg increased in summer, potentially in response to enhanced microbial production in warmer periods. MeHg formation may have been linked to protein‐like DOM, but its transport was linked to humic‐like DOM.
Stereotactic body radiotherapy of adrenal metastases—A dose‐finding study
Authors: Buergy Daniel , Würschmidt Florian , Gkika Eleni , Hörner‐Rieber Juliane , Knippen Stefan , Gerum Sabine , Balermpas Panagiotis , Henkenberens Christoph , Voglhuber Theresa , Kornhuber Christine , Barczyk Steffen , Röper Barbara , Rashid Ali , Blanck Oliver , Wittig Andrea , Herold Hans‐Ulrich , Brunner Thomas Baptist , Sweeney Reinhart A. , Kahl Klaus Henning , Ciernik Ilja Frank , Ottinger Annette , Izaguirre Victor , Putz Florian , König Laila , Hoffmann Michael , Combs Stephanie Elisabeth , Guckenberger Matthias , Boda‐Heggemann Judit 
Published: April 2022

AbstractOptimal doses for the treatment of adrenal metastases with stereotactic radiotherapy (SBRT) are unknown. We aimed to identify dose‐volume cut‐points associated with decreased local recurrence rates (LRR). A multicenter database of patients with adrenal metastases of any histology treated with SBRT (biologically effective dose, BED10 ≥50 Gy, ≤12 fractions) was analyzed. Details on dose‐volume parameters were required (planning target volume: PTV‐D98%, PTV‐D50%, PTV‐D2%; gross tumor volume: GTV‐D50%, GTV‐mean). Cut‐points for LRR were optimized using the R maxstat package. One hundred and ninety‐six patients with 218 lesions were included, the largest histopathological subgroup was adenocarcinoma (n = 101). Cut‐point optimization resulted in significant cut‐points for PTV‐D50% (BED10: 73.2 Gy; P = .003), GTV‐D50% (BED10: 74.2 Gy; P = .006), GTV‐mean (BED10: 73.0 Gy; P = .007), and PTV‐D2% (BED10: 78.0 Gy; P = .02) but not for the PTV‐D98% (P = .06). Differences in LRR were clinically relevant (LRR ≥ doubled for cut‐points that were not achieved). Further dose‐escalation was not associated with further improved LRR. PTV‐D50%, GTV‐D50%, and GTV‐mean cut‐points were also associated with significantly improved LRR in the adenocarcinoma subgroup. Separate dose optimizations indicated a lower cut‐point for the PTV‐D50% (BED10: 69.1 Gy) in adenocarcinoma lesions, other values were similar (<2% difference). Associations of cut‐points with overall survival (OS) and progression‐free survival were not significant but durable freedom from local recurrence was associated with OS in a landmark model (P < .001). To achieve a significant improvement of LRR for adrenal SBRT, a moderate escalation of PTV‐D50% BED10 >73.2 Gy (adenocarcinoma: 69.1 Gy) should be considered.
Amniotic growth factors enhanced human pre‐adipocyte cell viability and differentiation under hypoxia
Authors: Magana Alejandro , Giovanni Regina , Essien Edidiong , Epel Boris , Kotecha Mrignayani , Liu Songyun , Mathew Mathew T. , Hagarty Sarah E. , Bijukumar Divya 
Published: April 2022

AbstractOne of the major drawbacks associated with autologous fat grafting is unpredictable graft retention. Various efforts to improve the survivability of these cells have been explored, but these methods are time‐consuming, complex, and demand significant technical skill. In our study, we examine the use of cryopreserved amniotic membrane as a source of exogenous growth factors to improve adipocyte survivability under normal and hypoxic conditions. Human primary preadipocytes were cultured in a gelatin‐ferulic acid (Gtn‐FA) hydrogel with variable oxygen concentration and treated with amniotic membrane‐derived condition medium (CM) for 7 days. This hydrogel provides a hypoxic environment and also creates a 3D cell culture to better mimic recipient site conditions. The O2 concentration in the hydrogel was measured by electron paramagnetic resonance oxygen imaging (EPROI). The conjugation of FA was confirmed by FTIR and NMR spectroscopy. The cell viability and adipocyte differentiation were analyzed by alamarBlue™ assay, Oil Red O staining, and RT‐qPCR. The expression of genes: Pref‐1, C/EBP β, C/EBP α, PPAR‐ƴ, SLC2A4, and VEGF‐A were quantified. The cell viability results show that the 50% CM showed significantly higher cell pre‐adipocyte cell viability. In addition, compared to normal conditions, hypoxia/CM provided higher PPAR‐ƴ (p < .05), SLC2A4, and VEGF‐A (p < .05) (early and terminal differentiating markers) mRNA expression. This finding demonstrates the efficacy of amniotic CM supplementation as a novel way to promote adipocyte survival and retention via the expression of key gene markers for differentiation and angiogenesis.
Deletion of autophagy related, ATG1 and F‐box motif encoding YDR131C, together, lead to synthetic growth defects and flocculation behavior in Saccharomyces cerevisiae
Authors: Shoket Heena , Parvez Sadia , Sharma Meenu , Pandita Monika , Sharma Vishali , Kumar Prabhat , Bairwa Narendra K. 
Published: April 2022

AbstractUbiquitin proteasome system (UPS) and autophagy both pathways are involved in clearing the nonessential cellular components and also crosstalk during cellular response to normal and stress conditions. The F‐box motif proteins constitute the SCF‐E3 ligase complex of the UPS pathway in Saccharomyces cerevisiae and are involved in the substrate recruitment for ubiquitination. The ATG1 encoded Atg1p, a conserved serine‐threonine kinase is crucial for the autophagy process. Here in this study, we report that loss of F‐box motif encoding YDR131C and ATG1 together results in growth defects, floc formation, sensitivity to hydroxyurea, methyl methanesulfonate, and hydrogen peroxide. Both the genes also interact with the flocculation‐related genes (FLO) and associate with gene ontology terms “ubiquitin‐protein transferase activity” and “cellular catabolic process.” Based on in silico analysis and experimental evidence we conclude that YDR131C and ATG1 function in parallel pathways to regulate the growth, flocculation, and stress response.
L1CAM‐associated extracellular vesicles: A systematic review of nomenclature, sources, separation, and characterization
Authors: Gomes Dimitria E. , Witwer Kenneth W. 
Published: April 2022

AbstractWhen released into biological fluids like blood or saliva, brain extracellular vesicles (EVs) might provide a window into otherwise inaccessible tissue, contributing useful biomarkers of neurodegenerative and other central nervous system (CNS) diseases. To enrich for brain EVs in the periphery, however, cell‐specific EV surface markers are needed. The protein that has been used most frequently to obtain EVs of putative neuronal origin is the transmembrane L1 cell adhesion molecule (L1CAM/CD171). In this systematic review, we examine the existing literature on L1CAM and EVs, including investigations of both neurodegenerative disease and cancer through the lens of the minimal information for studies of EVs (MISEV), specifically in the domains of nomenclature usage, EV sources, and EV separation and characterization. Although numerous studies have reported L1CAM‐associated biomarker signatures that correlate with disease, interpretation of these results is complicated since L1CAM expression is not restricted to neurons and is also upregulated during cancer progression. A recent study has suggested that L1CAM epitopes are present in biofluids mostly or entirely as cleaved, soluble protein. Our findings on practices and trends in L1CAM‐mediated EV separation, enrichment, and characterization yield insights that may assist with interpreting results, evaluating rigor, and suggesting avenues for further exploration.