bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbon nanoparticles induce DNA repair and PARP inhibitor resistance associated with nanozyme activity in cancer cells
Authors: Fan Haiyan , Sun Qinglei , Dukenbayev Kanat , Benassi Enrico , Manarbek Limara , Nurkesh Ayan A. , Khamijan Medina , Mu Chenglin , Li Guoliang , Razbekova Madina , Chen Zhenbang , Amin Amr , Xie Yingqiu 
Published: November 2022

Abstract
Background
Quantum nanodots especially carbon nanoparticles (CNPs) have been widely studied in biomedicine in imaging, drug delivery, but anti-cancer mechanisms remain elusive.
Methods
Here we studied on a type of cell death induced by food (beet, soybean) derived CNPs in cancer cells and tested whether CNPs induced DNA damage and resistant to anticancer agent PARP inhibitor (PARPi) could be overcome by quantum calculation, SEM, TEM, AFM, FT-IR, soft agar assay, and cytotoxicity assay.
Results
At high doses, CNPs derived from beet lead to a pop-like apoptosis of cells. Quantum mechanical calculation confirmed CNPs binding with phosphate groups as well as DNA bases. At low doses, CNPs develop PARPi drug resistance through interactions between CNPs and PARPi. A synergistic drug effect was achieved with the combination of phosphatase inhibitor (PPi), PARPi and CNPs. This is corroborated by the fact that a sulfur modulated CNPs which exhibit super high phosphatase nanozyme activity abrogated the CNPs induced colony formation in anchorage-independent cancer cell growth.
Conclusion
Thus, our data suggest the CNPs intrinsic nanozyme activity of phosphatase may crosstalk with drug resistance, which can be reversed upon modulations.
Effects and parameters of community-based exercise on motor symptoms in Parkinson's disease: a meta-analysis
Authors: Yang Chun-Lan , Huang Jia-Peng , Wang Ting-Ting , Tan Ying-Chao , Chen Yin , Zhao Zi-Qi , Qu Chao-Hua , Qu Yun 
Published: November 2022

Abstract

Background Community-based exercise is a continuation and supplement of Parkinson's hospital rehabilitation and is a type of unsupervised exercise. The effects, parameters, and forms of each study were diverse. The effect is affected by many factors. In this paper, a Meta-analysis was conducted to determine the effect and the best parameters for improving motor symptoms and explore the possible factors affecting the effect. Methods We conducted a comprehensive search of six databases: PEDro, PubMed, CENTRAL, Scopus, Embase, and WOS. Studies comparing community-based exercise with usual care were included. The intervention mainly included dance, Chinese martial arts, Nordic walking, and home-based exercise. The primary outcome measure was the Unified Parkinson’s Disease Rating Scale part III (UPDRS-III). Mean difference (95% CI) was used to calculate treatment outcomes of continuous outcome variables, and I2was used to estimate the heterogeneity of the statistical analysis. We conducted subgroup analysis and meta-regression analysis to determine the optimal parameters and the most important influencing factors of exercise effect. We previously registered the proposal of the meta-analysis on the PROSPERO website (NO. CRD42022327162) and strictly reported it according to PRISMA's stated terms. Results Twenty-two studies enrolling a total of 809 subjects were included in the analysis. Exercise had a positive effect on UPDRS-III (MD= -5.83; 95% CI, -8.29 to -3.37), Timed Up and Go Test (MD= -2.22; 95% CI -3.02 to -1.42), UPDRS ((MD= -7.80; 95% CI -10.98 to -6.42), 6-Minute Walk Test (MD = 68.81; 95% CI, 32.14 to 105.48), and Berg Balance Scale (MD = 4.52; 95% CI, 2.72 to 5.78). However, the heterogeneity of each study included was obvious. Weekly frequency, age, and location are all factors potentially affecting the effect. Conclusions This meta-analysis suggests that community-based exercise may benefit motor function in patients with PD. The most commonly used modalities of exercise are Tango and Tai Chi, and The most common prescription is 60 minutes twice a week. Future studies should consider the influence of age, region, and weekly frequency on the effect.PROSPERO trial registration numberCRD42022327162.
RNN-Fuzzy-c-means-smote and fuzzy classifier to early predict hidden disease
Authors: Roudani Mohammed , Moutaouakil Karim El 
Published: November 2022

Abstract

Unbalanced dataset classification issues have been prevalent in medical domain. Lately, clustering-based oversampling techniques were introduced to address this issue. Regrettably, they suffer from the vulnerability of hard grouping approaches. This paper introduces Recurrent Neural Network Fuzzy-C-Means SMOTE (RNNFCM-SMOTE) that balances data based on Recurrent Neural Network Fuzzy Fuzzy-C-Means in filtering phase. First of all, to manage the sensitiveness of the hard clustering, RNNFCM is used to determine safe regions. Second, take benefit of the capacity of neural networks to comprehend the features of data and dynamic systems to recover from past clusters, recurrent neural network is implemented to determine the membership function of different instances. To generate artificial data, classical smote is used. As the methods implementing fuzzy logic have proven to be very competent when it comes to the edge problem, RNNFCM-SMOTE is combined to nine fuzzy classifier methods to predict the existence of hidden diseases represented by five unbalanced medical data sets. The proposed method is compared to 12 oversampling methods using three performance measures. RNNFCM-SMOTE has been shown to consistently exceed many other popular oversampling techniques
Comparison of Climate Change Impact upon Water Productivity of Winter Wheat in Northern and Southern Regions of Iraq
Authors: Hasan Ban Flaieh , Abed Basim Sh. 
Published: November 2022

Abstract
Maximizing the water productivity for any agricultural system is considered an adaptation to the potential climate change crisis. It is required, especially in arid and semi-arid environments in Iraq. Therefore, this study assessed the potential impact of climate change on the different environments in the Qadissiya and Nineveh provinces. The ensemble of six GCM models employed for the regional climate model of the HCLIM-ALADIN in high-resolution 10*10 km2 and Aqua-Crop was used to examine the response of water productivity and yield of winter wheat. With and without CO2 concentration changing under different water regimes in the near term (2020-2040) and mid-term (2041-2060) related to the reference period (1995-2014). The model was validated in both provinces to indicate good performance of RRMSE (4.54- 7.1) and d, R2 ( 1- 0.99). The main findings revealed an increase in water productivity, yield production, and transpiration reduction under the CO2-changing scenario and behaved stable under the fixed concentration scenario. The developed schedule enhanced water productivity in both locations. The assessment study examined the resilience of arid and semi-arid agricultural lands under future climate change.
A Modified Two-step Technique for the Retrieval of a Chemoport Catheter Fragment With Inaccesible Ends
Authors: Sokwalla Naushad Karim , Sagoo Ravjit , Moussa Alel , Mansour Manel 
Published: November 2022

Abstract
BACKGROUND
Migration of central venous catheters is a rare but serious complication. The endovascular approach has been widely used for the retrieval of such fragment, with the two-step technique used for removal of catheter fragments with inaccessible ends. In this case report, we describe a modification of this technique that was used after first attempting the two-step technique unsuccessfully.
CASE PRESENTATION:
A 42-year-old female with breast cancer had a chemoport inserted for chemotherapy. After 6 cycles of chemotherapy the port could not be flushed and a chest radiograph demonstrated a migrated catheter fragment. CT scan demonstrated that one end of the fragment was in the liver in the middle hepatic vein and the other in the right atrial appendage. A modified 2 step technique, using a pigtail catheter, hydrophilic wire and snare was used to remove this fragment.
CONCLUSION:
In this case report we highlight a new modification of the 2-step technique that can be employed when the conventional 2 step technique does not work.
Association of Karnofsky Performance Status with liver transplant outcomes in hepatocellular carcinoma
Authors: Zhou Jie , Ye Danni , Zhang Siyao , Ding Jiawei , Zhang Tao , Chen Zheng , Xu Fangshen , Ren Shenli , Hu Zhenhua 
Published: November 2022

Abstract
Background
Functional performance as measured by the Karnofsky Performance Status (KPS) scale has been linked to the outcomes of liver transplant patients; however, the effect of KPS on the outcomes of the hepatocellular carcinoma (HCC) liver transplant population has not been fully elucidated. We aimed to investigate the association between pre-transplant KPS score and long-term outcomes in HCC patients listed for liver transplantation.
Methods
Adult HCC candidates listed on the Scientific Registry of Transplant Recipients (SRTR) database from January 1, 2011 to December 31, 2017 were grouped into KPS I (80–100%, n = 8,379), KPS II (50–70%, n = 8,091), and KPS III (10–40%, n = 1,256) based on percentage KPS score at listing. Survival was compared and multivariable analysis was performed to identify independent predictors.
Results
Patients with low KPS score had a higher risk of removal from the waiting list. The intent-to-treat survival and overall survival were significantly inferior for KPS III patients compared with KPS I and II (P < 0.001). Multivariable analysis demonstrated that KPS was an independent predictor of intent-to-treat survival and overall survival. KPS III patients had an increased cumulative rate of post-transplant tumor recurrence in the univariable analysis (P = 0.037), but this was not significant in the competing regression analysis.
Conclusions
Low pre-transplant KPS score is associated with inferior long-term survival in liver transplant HCC patients, but is not significantly associated with post-transplant tumor recurrence.
Combination Immunotherapy of Glioblastoma with Dendritic Cell Cancer Vaccines, Anti-PD-1 and Poly I:C: A Case Report
Authors: He You-Wen , Zhu Ping , Sun Sheng-Nan , Ding Jin , Zheng Zhao-Hui , Wei Ding , Jiang Jun , Miao Jin-Lin , Li San-Zhong , Fei Zhou , Zhang Kui , Wang Bin , Zhang Kun , Pu Su , Wang Qian-Ting , Zhang Xin-Yue , Wen Gao-Liu , Li Shi-You , Bian Hui-Jie , Chen Zhi-Nan , Liu Jun 
Published: November 2022

Abstract
Glioblastoma (GBM) is a lethal cancer with limited therapeutic options. Dendritic cell (DC)-based cancer vaccines represent a promising approach for GBM treatment. Clinical studies suggest that other immunotherapeutic agents including anti-PD-1 and immune adjuvant poly I:C may be combined with DC vaccines to further enhance antitumor activity. Here we report a GBM case with combination immunotherapy consisting of DC vaccines, anti-PD-1 and poly I:C as well as a chemotherapeutic agent cyclophosphamide that were integrated with the standard chemoradiation therapy for > 5 years. The patient who received DC vaccines loaded with multiple forms of tumor antigens including mRNA-tumor associated antigens (TAAs), mRNA-neoantigens, and hypochlorous acid-oxidized tumor lysates. Furthermore, mRNA-TAAs were modified with a novel TriVac technology which fuses TAAs with a destabilization domain and inserts TAAs into a full-length LAMP-1 to enhance MHC class I and II antigen presentation, respectively. The treatment consisted of 42 DC cancer vaccine infusions, 26 anti-PD-1 antibody nivolumab administrations and 126 poly I:C injections for DC infusions. The patient also received 28 doses of cyclophosphamide for depletion of regulatory T cells. No immunotherapy related adverse events were observed during the treatment. Robust anti-tumor CD4+ and CD8+ T cell responses were detected. The patient remains free of disease progression as of July, 2022. Our results suggest that integrated combination immunotherapy is safe and feasible for long-term treatment. A large scale trial to validate these findings is warranted.
Protocol numbers: 201708152377, 20202014-F-1
Evaluation of a thrombin generation assay in dogs administered clopidogrel
Authors: Rank Kaitlyn , Lynch Alex , Ruterbories Laura , Li Ronald , Ueda Yu 
Published: November 2022

Abstract
Background:
The antiplatelet effect of clopidogrel can vary between patients. A modified thromboelastography (TEG) protocol (TEG-Platelet Mapping assay® [TEG-PM]) can be used for clopidogrel monitoring but is not widely available. Thrombin generation (TG) assays could offer a novel alternative. The main objective of this pilot study was to assess TG assay variables (lag time, peak, endogenous thrombin potential [ETP]) in dogs before and after 7 days of clopidogrel administration, and compare with TEG-PM variables (maximum amplitude [MA]-ADP and percentage (%) inhibition). Six healthy mix-breed dogs were enrolled in this pilot study. Blood samples for platelet count, TG assays, and TEG-PM were obtained at two time points, corresponding to baseline, and after 7 days of clopidogrel administration (mean 2.3 +/- 0.3 mg/kg PO q24 hours). Data were then compared with a Student’s t-test.
Results
There was no significant change in TG assay variables performed on platelet poor plasma after 7 days of clopidogrel administration: lag time (Day 1: 1.8 +/- 0.2 min, Day 7: 1.8 +/- 0.2 min, P = 0.42); Peak (Day 1: 76 +/- 7 nM, Day 7: 72 +/- 10 nM, P = 0.49); and ETP (Day 1: 399 +/- 27 nM*min, Day 7: 392 +/- 32 nM*min; P = 0.49). There were significant changes in TEG MA-ADP (Day 1: 19 +/- 8 mm, Day 7: 9 +/- 6 mm, P = 0.04) and % inhibition (Day 1: 58 +/- 27, Day 7: 99 +/- 0.3, P = 0.02) however over the course of the study.
Conclusions
Clopidogrel administration did not lead to changes in TG assay variables performed on platelet poor plasma samples, despite concomitant changes in TEG-PM variables consistent with platelet inhibition. Thrombin generation performed on platelet poor plasma does not appear to be a useful antiplatelet monitoring tool in dogs.
Integrated analysis of the microbiota-gut-brain axis in response to sleep deprivation in a diet-induced obesity mouse model
Authors: Oh Chang Myung , Lee Jibeom , Kang Jiseung , Kim Yumin , Lee Sunjae , Kim Tae 
Published: November 2022

Abstract
Sleep deprivation (SD) and obesity are common in modern societies. SD and obesity frequently coexist, but research on the combined consequences of SD and obesity has been limited. In this study, we investigated the gut microbiota and host responses to SD and high-fat diet (HFD)-induced obesity. In addition, we attempted to identify key mediators of the microbiota-gut-brain axis. C57BL/6J mice were divided into four groups based on whether they were sleep deprived and whether they were fed a standard chow diet (SCD) or HFD. We then performed fecal microbiome shotgun sequencing, gut transcriptome analysis using RNA sequencing, and brain mRNA expression analysis using the nanoString nCounter Mouse Neuroinflammation Panel. The gut microbiota was significantly altered by the HFD, whereas the gut transcriptome was primarily influenced by SD. Sleep and diet are both important in the inflammatory system of the brain. When SD and the HFD were combined, the inflammatory system of the brain was severely disrupted. In addition, inosine-5' phosphate may be the gut microbial metabolite that mediates microbiota-gut-brain interactions. To identify the major drivers of this interaction, we analyzed the multi-omics data. Integrative analysis revealed two driver factors that were mostly composed of the gut microbiota. We discovered that the gut microbiota may be the primary driver of microbiota-gut-brain interactions. These findings imply that healing gut dysbiosis may be a viable therapeutic target for enhancing sleep quality and curing obesity-related dysfunction.
Microsatellite polymorphism based genetic variation among the natural populations of two sympatric species of Drosophila: D. bipectinate and D. malerkotliana
Authors: Singh Gurvachan , Singh Arvind Kumar 
Published: November 2022

Abstract
Background
Drosophila bipectinata and D. malerkotliana are found to be distributed all along the Oriental-Australian continents and have common phylogenetic descent. The genetic divergence of these two species, alongside their distribution zone have been the matter of research and therefore, genetic markers like chromosome inversions and allozyme variants have been considered to establish the nature and extent of variation they have experienced since their speciation.
Methods and Results
This study for the first time includes microsatellite markers to observe the genetic variations among the seven natural populations of D. bipectinata and six populations of D. malerkotliana. Results pertaining to D. bipectinata indicate that the level of average heterozygosity increases from north to south. Similar analysis carried out with D. malerkotliana also showed an increase in the level of average heterozygosity from north to south. Distinct genetic differentiation has been observed between north and south populations of both the species that give an indication that north and south populations have undergone genetic structuring due to absence of gene flow between them and varying environmental conditions of the two geographical zones. Based on the frequencies of microsatellite variants of the two species, we have computed the level of variation between these two species also.
Conclusions
The analysis in this regard shows almost similar trend of genetic variation being experienced by these two species which could be a kind of parallel evolution occurring due to similar ecological niches occupied by them and similar impact of evolutionary forces to the populations studied.
Single cell profiling reveals a reduction of CD56dim NK-cells and other immune cells in patients with Meniere disease and high levels of cytokines
Authors: Flook Marisa , Escalera-Balsera Alba , Rybakowska Paulina , Frejo Lidia , Batuecas-Caletrio Angel , Amor-Dorado Juan Carlos , Soto-Varela Andres , Alarcón-Riquelme Marta , Lopez-Escamez Jose Antonio 
Published: November 2022

Abstract
Background
Meniere Disease (MD) is an inner ear syndrome, characterized by sensorineural hearing loss associated with episodes of vertigo, tinnitus, and aural fullness. The pathological mechanism leading to sporadic MD is still poorly understood, however an inflammatory response seems to be involved in some patients with MD.
Methods
We performed mass cytometry immunoprofiling on peripheral blood from MD patients and controls to decipher an immune signature associated with the syndrome.
Results
We have identified two clusters of individuals according to the single cell cytokine profile. These clusters presented differences in immune cell population abundance, including a reduction of CD56dim NK-cells, and changes in cytokine expression with a different response to bacterial and fungal antigens. Moreover, we observe a difference in the levels of IgE between these two clusters.
Conclusions
Our results support a systemic inflammatory response in some MD patients that show an allergic phenotype, which could benefit from personalized IL-4 blockers. Immunoprofiling of patients with MD may lead to a better understanding of endotypes of the disease.
Failure to integrate? Investigating multisensory acceleration perception on a cable-robot simulator
Authors: Kang Hyeokmook , Yang Jaesik , Boss Rainer , Bülthoff Heinrich H , Wallraven Christian 
Published: November 2022

Abstract
Current powertrains in vehicles offer a broad spectrum of driving modes that shape the driving experience. One crucial factor for determining the human factors aspects of this driving experience is the perception and evaluation of acceleration-a complex, multisensory process that integrates auditory, vestibular, and visual input. Two important questions that need to be answered in order to better characterize this percept include: How do individual sensory inputs influence acceleration perception? and How does acceleration perception change at different acceleration levels? To address these questions, here we used a unique setup based on a cable-robot simulator that allowed us to manipulate the different modalities at different levels of acceleration with real-world, in-car data for maximum realism. Specifically, we measured the just noticeable differences (JNDs) of acceleration perception in five different modality combinations with the same set of participants. Our results showed that auditory acceleration perception was less sensitive compared to other modality combinations. In addition, we found evidence for the validity of Weber’s law with JNDs increasing linearly with increasing acceleration level. Interestingly, the multisensory data showed little evidence for effective cue integration of auditory information in this setup. These findings lay the groundwork for a better understanding of how different modalities work together in acceleration perception.
The possible protective effects of quercetin and/ or resveratrol against acrolein–induced sister chromatid exchanges and DNA damage in male albino mice.
Authors: El-Alfy Nagla , Mahmoud Mahmoud , Elashry Sally 
Published: November 2022

Abstract
Quercetin and resveratrol, common plant polyphenols, are widely distributed in human daily food and can contribute in the protection against the genotoxic toxic effects of various food contaminants and environmental pollutants. The aim of this study is to investigate the protective role of quercetin (50 mg/ kg body weight) and/ or resveratrol (12.5 mg/ kg body weight) against the genotoxicity of acrolein (10 mg/ kg body weight) in male albino mice using sister chromatid exchanges (SCEs) analysis and comet assay. The SCE analysis revealed that acrolein treatment significantly increased the SCEs frequency in bone marrow cells of acrolein-treated group in comparison with that of the control group, whereas pre-treatment with quercetin and/ or resveratrol significantly reduced it. Comet assay showed a significant increase in the comet parameters (tail length, tail DNA% and olive tail moment) in liver cells of acrolein-treated group in comparison with the corresponding control. In quercetin and/ or resveratrol pre-treated groups, the comet parameters were reduced in liver cells, indicating the anti-genotoxicity of quercetin and resveratrol. Pre-treatment with quercetin alone showed the best anti-genotoxic effect against acrolein-toxicity. Therefore, quercetin and resveratrol should be included in the human diet to antagonize the dangerous effects of mutagens like acrolein.
Breeding biology of isolated captive Dalmatian Pelicans (Pelecanus crispus) at the Shanghai Zoo, China
Authors: Xu Zhengqiang , Zhou Ying , Chen Zhibing 
Published: November 2022

Abstract
The breeding biology of a captive, isolated population of Dalmatian Pelicans (Pelecanus crispus) at the Shanghai Zoo, China, was studied from 2007 to 2019. The breeding age of the Dalmatian Pelicans was estimated at three to four years old, and they started breeding in late October or early November. The clutch size varied between one and two eggs per nest, and the eggs were 83.2 ± 4.8 mm in length, 55.6 ± 2.9 mm in breadth and 136.1 ± 21.5 g in weight. The fertility fluctuated slightly, with an average rate of 38.7 ± 9.7%, but remained consistently low. The survival rate of chicks fluctuated substantially from 0–100%, with an average survival rate of 68.6 ± 27.6%. The averaged values of observed heterozygosity, expected heterozygosity and polymorphism information content were 0.468, 0.465 and 0.446, respectively. It indicated that the population had a low heterozygosity and genetic diversity. There was a significant change in the breeding index compared to that recorded 40 years ago, which indicates that inbreeding depression has occurred in this small, isolated, captive population of Dalmatian Pelicans.