bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen breaks at weak sacrificial bonds taming its mechanoradicals
Authors: Rennekamp Benedikt , Karfusehr Christoph , Kurth Markus , Ünal Aysecan , Riedmiller Kai , Gryn’ova Ganna , Hudson David M. , Gräter Frauke 
Published: October 2022

AbstractCollagen is a force-bearing, hierarchical structural protein important to all connective tissue. In tendon collagen, high load even below macroscopic failure level creates mechanoradicals by homolytic bond scission, similar to polymers. The location and type of initial rupture sites critically decide on both the mechanical and chemical impact of these micro-ruptures on the tissue, but are yet to be explored. We here use scale-bridging simulations supported by gel electrophoresis and mass spectrometry to determine breakage points in collagen. We find collagen crosslinks, as opposed to the backbone, to harbor the weakest bonds, with one particular bond in trivalent crosslinks as the most dominant rupture site. We identify this bond as sacrificial, rupturing prior to other bonds while maintaining the material’s integrity. Also, collagen’s weak bonds funnel ruptures such that the potentially harmful mechanoradicals are readily stabilized. Our results suggest this unique failure mode of collagen to be tailored towards combatting an early onset of macroscopic failure and material ageing.
A network of small RNAs regulates sporulation initiation inC. difficile
Authors: Fuchs Manuela , Lamm-Schmidt Vanessa , Lenče Tina , Sulzer Johannes , Bublitz Arne , Gerovac Milan , Strowig Till , Faber Franziska 
Published: October 2022

ABSTRACTThe obligate anaerobic, enteric pathogenClostridioides difficilepersists in the intestinal tract by forming antibiotic resistant endospores that contribute to relapsing and recurrent infections. Despite the importance of sporulation forC. difficilepathogenesis, environmental cues, and molecular mechanisms regulating sporulation initiation remain ill defined. Here, using RIL-seq to capture the Hfq-dependent RNA-RNA interactome, we discovered a network of small RNAs that bind to mRNAs encoding sporulation-related genes. We show that two of these small RNAs, SpoX and SpoY, regulate translation of the master regulator of sporulation, Spo0A, in an opposing manner, which ultimately leads to altered sporulation rates. Infection of antibiotic-treated mice with SpoX and SpoY deletion mutants revealed a global effect on gut colonization and intestinal sporulation. Our work uncovers an elaborate RNA-RNA interactome controlling the physiology and virulence ofC. difficileand identifies a complex post-transcriptional layer in the regulation of spore formation in this important human pathogen.
Cell size contributes to single-cell proteome variation
Authors: Lanz Michael C. , Valenzuela Lucas , Elias Joshua E. , Skotheim Jan M. 
Published: October 2022

AbstractAccurate measurements of the molecular composition of single cells will be necessary for understanding the relationship between gene expression and function in diverse cell types. One of the most important phenotypes that differs between cells is their size, which was recently shown to be an important determinant of proteome composition in populations of similarly sized cells. We therefore sought to test if the effects of cell size on protein concentrations were also evident in single cell proteomics data. Using the relative concentrations of a set of reference proteins to estimate a cell’s DNA-to-cell volume ratio, we found that differences in cell size explain a significant amount of cell-to-cell variance in two published single cell proteome datasets.
Cellular and environmental dynamics influence species-specific extents of organelle gene retention
Authors: García Pascual Belén , Nordbotten Jan M. , Johnston Iain G. 
Published: October 2022

AbstractMitochondria and plastids rely on many nuclear-encoded genes, but retain small subsets of the genes they need to function in their own organelle DNA (oDNA). Different species retain different numbers of oDNA genes, and the reasons for these differences are not completely understood. Here we use a mathematical model to explore the hypothesis that the energetic demands imposed by an organism’s changing environment influence how many oDNA genes it retains. The model couples the physical biology of cell processes of gene expression and transport to a supply-and-demand model for the environmental dynamics to which an organism is exposed. The tradeoff between fulfilling metabolic and bioenergetic environmental demands, and retaining genetic integrity, is quantified for a generic gene encoded either in oDNA or in nuclear DNA. Species in environments with high-amplitude, intermediate-frequency oscillations are predicted to retain the most organelle genes, whereas those in less dynamic or noisy environments the fewest. We discuss support for, and insight from, these predictions with oDNA data across eukaryotic taxa, including high oDNA gene counts in sessile organisms exposed to day-night and intertidal oscillations (including plants and algae) and low counts in parasites and fungi.
Surface phenotyping and quantitative proteomics reveal differentially enriched proteins of brain-derived extracellular vesicles in Parkinson’s disease
Authors: Arab Tanina , Huang Yiyao , Nagaraj Rajini , Gizzie Evan , Redding-Ochoa Javier , Troncoso Juan C. , Pletnikova Olga , Boronina Tatiana , Cole Robert N. , Mahairaki Vasiliki , Routenberg David A. , Witwer Kenneth W. 
Published: October 2022

ABSTRACTExtracellular vesicles (EVs) are produced by all cell types and are found in all tissues and biofluids. EV proteins, nucleic acids, and lipids are a “nano-snapshot” of the parent cell that may be used for novel diagnostics of various diseases, including neurodegenerative disorders. Currently, diagnosis of the most common neurodegenerative movement disorder, Parkinson’s disease (PD), relies on manifestations of late-stage progression, which may furthermore associate with other neurodegenerative diseases such as progressive supranuclear palsy (PSP). Here, we profiled surface markers and other protein contents of brain-derived extracellular vesicles (bd-EVs) from PD (n= 24), PSP (n=25) and control (n=24). bdEVs displayed tetraspanins and certain microglia, astrocyte, and neuron markers, while quantitative proteomics revealed enrichment of several proteins in PD vs. control and/or PSP, including clathrin heavy chain 1 and 14-3-3 protein gamma. This characterization of EVs in the source tissue provides insights into local dynamics as well as biomarker candidates for investigation in peripheral fluids.
Associated tolerance optimization approach using manufacturing difficulty coefficients and genetic algorithm
Authors: Ghali Maroua , Elghali Sami , Aifaoui Nizar 
Published: October 2022

Purpose
The purpose of this paper is to establish a tolerance optimization method based on manufacturing difficulty computation using the genetic algorithm (GA) method. This proposal is among the authors’ perspectives of accomplished previous research work to cooperative optimal tolerance allocation approach for concurrent engineering area.

Design/methodology/approach
This study introduces the proposed GA modeling. The objective function of the proposed GA is to minimize total cost constrained by the equation of functional requirements tolerances considering difficulty coefficients. The manufacturing difficulty computation is based on tools for the study and analysis of reliability of the design or the process, as the failure mode, effects and criticality analysis (FMECA) and Ishikawa diagram.

Findings
The proposed approach, based on difficulty coefficient computation and GA optimization method [genetic algorithm optimization using difficulty coefficient computation (GADCC)], has been applied to mechanical assembly taken from the literature and compared to previous methods regarding tolerance values and computed total cost. The total cost is the summation of manufacturing cost and quality loss. The proposed approach is economic and efficient that leads to facilitate the manufacturing of difficult dimensions by increasing their tolerances and reducing the rate of defect parts of the assembly.

Originality/value
The originality of this new optimal tolerance allocation method is to make a marriage between GA and manufacturing difficulty. The computation of part dimensions difficulty is based on incorporating FMECA tool and Ishikawa diagram This comparative study highlights the benefits of the proposed GADCC optimization method. The results lead to obtain optimal tolerances that minimize the total cost and respect the functional, quality and manufacturing requirements.
It's a gift – Samuel Pepys and Sir William Warren, an account of gifts, bribes and kickbacks
Authors: McBride Karen 
Published: October 2022

PurposeThis study aims to investigate an individual’s boundary crossing with regard to bribery, gifts and favouritism in rewarding contracts. Samuel Pepys’s diary was written in the 17th century and through detailed accounts gives insight into his inherent professionalism and his negative opinions around bribery but also his acceptance of “gifts” and awarding of contracts to “friends”.Design/methodology/approachThe research uses a narrative approach to study a detailed and reflective personal diary identifying the diarist’s self-exploration and attitudes around the receipt of gifts and the awarding of contracts. This microhistory is presented with a narrative account of a case study of the relationship between the diarist, Pepys and a supplier, Sir W. Warren.FindingsThe diary illustrates how Pepys crosses these boundaries and how the lack of accountability within his role enables him to do this. This detailed study enables answers to questions that with time, legislation and lack of acceptability, have become more difficult to ask and to answer, about the crossing of boundaries and ethical decisions around the acceptance of bribes and kickbacks.Originality/valueA contribution of this paper is the use of a diary, at least a diary as self-reflective as Pepys’s written up as a narrative account. The use of a detailed diary in an accounting microhistory of this nature gives insight and assists in answering difficult to ask questions around personal motivations for bribery and corruption and contributes in this area. The research contributes in developing research around boundaries and the corruption equation using the insight gleaned from this narrative account.
Moral identity, consumption values and green purchase behaviour
Authors: Rana S.M. Sohel , Solaiman Mohammad 
Published: October 2022

Purpose
This study aims to explore the determinants of the green purchase behaviour (GPB) of environment-friendly and energy-efficient electronic products market. It specifically examines the moderating effect of consumers’ moral identity on the relationships between the consumption values and GPB of environment-friendly and energy-efficient electronic products market. It also examines the direct relationship between consumption values and GPB.

Design/methodology/approach
In this study, the theory of consumption values is combined with the moral identity of consumers. A structured questionnaire mall-intercept survey was used to collect data from 396 respondents, which was subsequently processed using the smart PLS software for partial least square structural equation modelling analysis.

Findings
Findings reveal that functional value, social value, conditional value and epistemic value are the significant predictors of GPB of the environment-friendly and energy-efficient electronic products market. The moral identity of consumers also appears to positively moderate the relationships between functional, emotional and conditional values and the GPB.

Originality/value
The energy efficiency of electronic products is included in this study as an additional feature of functional value, while government support and business promotional initiatives are incorporated as the new elements of conditional value. Therefore, the inclusion and evaluation of the moral identity of consumers, alongside new elements of functional and conditional values in the theory of consumption values, could be considered a significant theoretical addition. The study uncovered certain customer insights that could help accelerate the adoption of green electronic products, which may result in better energy savings, reduced carbon emissions and environmental safety.
Resources and capabilities for Industry 4.0 implementation: evidence from proactive Portuguese SMEs
Authors: Santos Beatrice , Dieste Marcos , Orzes Guido , Charrua-Santos Fernando 
Published: October 2022

PurposeThis paper seeks to shed light on the resources and capabilities required by SMEs to successfully implement Industry 4.0 (I4.0) and to explore how these resources and capabilities can be acquired and/or developed.Design/methodology/approachThe authors employed an exploratory multiple case study approach to analyze five Portuguese SMEs that have implemented I4.0 technologies. Data were primarily collected through direct semi-structured interviews with managers from different departments and areas. Moreover, a technology, organization and environment (TOE) framework approach was adopted to analyze the resources and capabilities needed for I4.0 implementation in SMEs.FindingsResearch findings suggest that the analyzed Portuguese SMEs did not require all the considered I4.0 resources and capabilities. Therefore, results may support practitioners to recognize and prioritize the resources and capabilities needed to successfully embrace all the benefits of I4.0. In this regard, SMEs can develop these resources and capabilities needed both internally (e.g. through the process and product innovation focused on digital technologies, human resource management practices and top management commitment) and externally (e.g. hiring skilled employees and through innovative collaboration networks).Originality/valueUsing the TOE framework, this study shows how SMEs could acquire and develop their resources and capabilities to accelerate I4.0 implementation and maximize its benefits.
Online service failure: antecedents, moderators and consequences
Authors: Adil Mohd , Sadiq Mohd , Jebarajakirthy Charles , Maseeh Haroon Iqbal , Sangroya Deepak , Bharti Kumkum 
Published: October 2022

PurposeThe purpose of this study is to present a systematic review of the online service failure (OSF) literature and conduct an exhaustive analysis of academic research on this emerging research area.Design/methodology/approachThe current study has adopted a structured systematic literature review approach to synthesize and assess the OSF literature. Further, the study uses the Theory-Context-Characteristics-Methodology (TCCM) framework to propose future research directions in the OSF domain.FindingsThis systematic review shows that OSF research is still developing and remains mainly incoherent. Further, the study develops a conceptual framework integrating the frequently reported antecedents, mediators, moderator and consequences in the extant literature. This review also synthesizes the theoretical perspectives adopted for this domain.Research limitations/implicationsThe study followed specific inclusion and exclusion criteria to shortlist articles. Further, articles published only in the English language were considered. Hence, the findings of this review cannot be generalized to all OSF literature.Practical implicationsThis systematic review has classified antecedents into customers' and service providers' roles which will enable online service providers to understand all sets of factors driving OSF. It also synthesizes and presents service recovery strategies and emphasizes the role of online customer support to fix OSF.Originality/valueThe OSF literature is still developing and remains highly incoherent, suggesting that a synthesized review is needed. This study has systematically reviewed and synthesized the OSF literature to study its development over time and proposes a framework which provides a comprehensive understanding of OSF.
Pay-for-performance, procedural justice, OCB and job performance: a sequential mediation model
Authors: Gupta Vishal , Mittal Shweta , Ilavarasan P. Vigneswara , Budhwar Pawan 
Published: October 2022

PurposeBuilding on the arguments of expectancy theory and social exchange theory, the present study provides insights into the process by which pay-for-performance (PFP) impacts employee job performance.Design/methodology/approachBased on a sample size of 226 employees working in a technology company in India, the study examines the relationships between PFP, procedural justice, organizational citizenship behavior (OCB) and employee job performance. Data on perceptions of PFP and procedural justice were collected from the employees, data on OCB were collected from the supervisors and the data on employee job performance were collected from organizational appraisal records.FindingsThe study found support for the positive relationship between PFP and job performance and for the sequential mediation of the relationship between PFP and job performance via procedural justice and OCB. Further, procedural justice was found to mediate the relationship between PFP and OCB.Research limitations/implicationsThe study was cross-sectional, so inferences about causality are limited.Practical implicationsThe study tests the relationship between PFP and employee job performance in the Indian work context. The study shows that the existence of PFP is positively related to procedural justice which, in turn, is positively related to OCB. The study found support for the sequential mediation of PFP-job performance relationship via procedural justice and OCB.Originality/valueThe study provides an insight into the underlying process through which PFP is related to employee job performance. To the best of our knowledge, such a study is the first of its kind undertaken in an organizational context.
Evaluation of effect and optimizing of process parameters for fused deposition modeling parts on tensile properties via Taguchi method
Authors: Demir Sermet , Yüksel Caner 
Published: October 2022

Purpose
The purpose of this paper is to analyze the effect of printing parameters on the mechanical properties of standard dog bone specimens manufactured by fused deposition modeling.

Design/methodology/approach
Polylactic acid (PLA) specimens were printed and tested according to the ASTM standard. The effect of five important printing parameters, layer height, raster angle, printing speed, nozzle temperature and nozzle diameter, was examined on ultimate tensile strength (UTS), elongation and apparent density. Five levels were attended for each parameter, and a high number of required experiments were reduced by applying the L25 Taguchi design of the experiment.

Findings
The effect of each parameter on outputs and optimal values for maximum tensile strength were determined. The most influential parameter is the raster angle of 64.96%. Nozzle temperature has a low effect of 1.76%, but nozzle diameter contribution is 9.77%. The experiment results are validated by analysis of variance analysis, and the optimal predicted level for parameters is 90° raster angle, 0.2 mm layer height, 100 mm/s printing speed, 200°C nozzle temperature and 0.8 mm nozzle diameter. The maximum UTS observed is 48.70 MPa for 0.8 mm nozzle diameter, whereas the minimum is 18.49 for 0.2 mm nozzle diameter.

Originality/value
This paper is a very extensive experimental research report on the effect of the parameters for the tensile property of 3D printed PLA specimens by the Taguchi method. The documented results can be further developed for an optimization model to obtain a desired mechanical property with less variation and uncertainty in a product.
Sundaic elephants prefer habitats on the periphery of protected areas
Authors: de la Torre J. Antonio , Cheah Cheryl , Lechner Alex M. , Wong Ee Phin , Tuuga Augustine , Saaban Salman , Goossens Benoit , Campos‐Arceiz Ahimsa 
Published: October 2022

Abstract

Protected areas (PAs) are a cornerstone of global conservation strategies. PAs, however, are not equally effective for all threatened taxa, and it is important to understand taxa‐specific effectiveness of PAs networks.

In this study, we evaluate the role of the PAs network on the protection of Asian elephants Elephas maximus and their habitats in Southeast Asia's Sundaic region. Since Asian elephants tend to prefer secondary forests or forest gaps, we predicted that PAs would not represent the species preferred habitats. We conducted the most comprehensive analysis of Asian elephant space and habitat use to date through home range estimations and step selection function analyses using over 600,000 Global Positioning System locations from 102 different elephants from Peninsular Malaysia and Borneo.

Our results revealed important similarities in the habitat use of elephants in both regions, with both females and males in Peninsular Malaysia and Sabah preferring secondary forest, forest gaps and areas of regrowth and new plantations.

Our results supported our prediction that PAs do not represent Asian elephants' preferred habitats, since for most of the elephants, more than half of their ranges were outside PAs and the probability of selection values for both sexes in both geographical areas were lower inside than outside the PAs.

Synthesis and applications. Our analysis suggests that conservation strategies need to acknowledge that the long‐term survival of Asian elephants in the Sundaic region relies on our capacity to promote human–elephant coexistence at the boundaries of PAs. We advocate that Asian elephant conservation strategies should be based on the following three key points: (1) large PAs with core areas where elephants can find safety and potentially survive in the long term; (2) promoting connectivity among PAs using a system of wildlife corridors; and (3) effective human–elephant conflict management outside PAs.
Marx on the Reciprocal Interconnections between the Soil and the Human Body: Ireland and Its Colonialised Metabolic Rifts
Authors: Slater Eamonn , Flaherty Eoin 
Published: October 2022

AbstractMarx’s writings on Ireland are widely known, but less appreciated is their centrality to the formation of his ecological thought. We show how Marx’s understanding of metabolic rift evolved in line with his writings on colonial Ireland, revealing a concept more holistic than the “classic” metabolic rift of the soil. We recover and extend this concept to the corporeal metabolic rift, showing how both are inherent in Marx’s various writings on Ireland. Whilst the rift of the soil concerns the extraction and consumption of organic soil constituents, the corporeal rift describes processes of depopulation, and their effects on demography and family formation. These “rifted” processes are interconnected such that depleted soil impacts on the health of those who consume food grown on those “rifted” soils. We argue that the presence of these rifts substantiates Ireland’s inability to sustain itself both economically and organically, which determined its persistent post‐Famine underdevelopment.