bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Electronic Medical Record Management Systems on the Disease Activity and Frequency of Outpatient Visits of Patients with Ankylosing Spondylitis: A Cross-Sectional Study
Authors: Huang Pei-Ju , Liu Yen-Tze , Chen Yi-Hsing , Chen Yi-Ming , Lai Kuo-Lung , Hsieh Tsu-Yi , Hung Wei-Ting , Lin Ching-Tsai , Tseng Chih-Wei , Tang Kuo-Tung , Chou Yin-Yi , Wu Yi-Da , Hsieh Chia-Wei , Chen Yen-Ju , Liao Yu-Wan , Chen Hsin-Hua , Huang Wen-Nan , Huang Chin-Yin 
Published: May 2022

Abstract
Background
To investigate the impact of an electronic medical record management system (EMRMS) on disease activity and the frequency of outpatient visits among patients with ankylosing spondylitis (AS).
Methods
We identified 652 patients with AS who were followed up for at least 1 year before and after the first Ankylosing Spondylitis Disease Activity Score (ASDAS) assessment and compared the number of outpatient visits and average visit time within 1 year before and after the initial ASDAS assessment. Finally, we analyzed 201 patients with AS who had complete data and received ≥ 3 continuous ASDAS assessments at an interval of 3 months, and we compared the results of the second and third ASDAS assessments with those of the first.
Results
The number of annual outpatient visits increased after ASDAS assessment (5.8 ± 3.4 vs. 5.4 ± 3.4, p < 0.001), particularly among those with a high initial disease activity. The average visit time was reduced within 1 year after ASDAS assessment (8.7 ± 3.8 vs. 9.2 ± 4.4 min, p = 0.030), especially among patients whose ASDAS-C-reactive protein (CRP) was < 1.3 (8.5 ± 3.3 vs. 9.2 ± 4.2 min, p = 0.022). Among patients who received at least three ASDAS assessments, the third ASDAS-CRP was significantly lower than the first (1.5 ± 0.8 vs. 1.6 ± 0.8, p = 0.049).
Conclusion
The use of an EMRMS increased the frequency of ambulatory visits among AS patients with high disease activity and reduced the visit time among those with an inactive disease. Continual ASDAS assessments may help control the disease activity of patients with AS.
Trial registration
Institutional Review Board (IRB) of Taichung Veterans General Hospital (TCVGH-IRB No.: CE20145B)
Is the Economic Benefit of Mixed Forest Better than Pure Forest Considering Harvesting Intensity and Carbon Payment?
Authors: Lin Yow-Ru , Liu Wan-Yu 
Published: May 2022

Abstract
Applying clear-cutting to an even-aged pure forest is a conventional forest operation for wood production. However, this kind of operation is not suitable for sustainable management with multiple objectives. In contrast, mixed-forest is a forestation strategy that accommodates diversity. The goal of this study was to assess an operation model that transforms from an even-aged pure forest into a mixed forest. Analysis in which an even-aged pure forest of Cryptomeria japonica was cut under a fixed harvesting intensity and replanted with the native broad-leaved tree species Cinnamomum camphora were conducted. In this study, the primary income for the forest owner was derived from wood sales and carbon payments. After deducting the costs of afforestation, management, and cutting, the land expectation value was estimated. The effects of critical variables, such as cutting time, harvesting intensity, and carbon price, were analyzed. This study explored strategies by which value could be increased in the transition from an even-aged pure forest to a mixed forest. Feasible methods included reducing the costs of afforestation, management, and cutting while increasing carbon prices to increase profits from wood and carbon income. Higher harvesting intensity could contribute to greater production of Cryptomeria japonica and increase the area available for ​​planting Cinnamomum camphora, resulting in greater profits from wood and carbon income. The net present value from market value would be from − 8,428 USD/ha to − 2,446 USD/ha and that from carbon value will be from 5,151 USD/ha to 6,895 USD/ha. However, this measure would also substantially affect forest ecology. A sole focus on increasing the production value of forestland will neglect the original goal of transforming from an even-aged pure forest into a mixed forest.
WOACCPP: Wisdom Of Artificial Crowds for Controller Placement Problem with Latency and Reliability in SDN-WAN
Authors: Thalapala Vidya Sagar , Mohan Anandhu , Guravaiah Koppala 
Published: May 2022

Abstract
The highly motivated Software-Defined Networking (SDN), the network programmability architecture made network maintenance easy. Multiple controllers are used to provide better scalability and reliability to the network in SDN. However, to enhance the network’s performance in networks at a large scale, the placement of controllers needs to be optimized. The Wisdom of Artificial Crowds (WOAC) genetic algorithm is used to minimize propagation latency by using the fewest possible controllers. To evaluate the performance of proposed technique the Internet2 OS3E topology is considered. The proposed controller placement technique reduces latency and improves reliability by comparing the existing solutions namely Modified Density Peak Clustering (MDPC) and optimized K-means approaches. The proposed method out performs existing techniques by 10% improves in latency and maximizes the reliability.
Genome-wide Identification of R2R3-MYB Gene Family and Response to Stress in Zingiber officinale Roscoe
Authors: Yao Xiaoyan , Meng Fei , Wu Liping , Guo Xiaohu , Sun Zongping , Jiang Weimin , Zhang Jing , Wu Jing , Wang Shuting , Wang Zhaojian , Su Xinglong , Dai Xiuru , Qu Changqing , Xing Shihai 
Published: May 2022

Abstract
Background: Zingiber officinale Roscoe (ginger), as a plant used for medicine and food, has a pivotal position all over the world. The R2R3-MYB transcription factor family is one of the largest, and it plays a significant role in plant growth, development, and stresses resistance. According to existing reports, the amounts of R2R3-MYB genes varies greatly among all kinds of plants. However, genome-wide discovery of ZoMYBs family and its corresponding stresses have not been reported in Z. officinale.Results: The genome-wide analysis of R2R3-MYB genes in Z. officinale was carried out in this study. Protein analysis included phylogenetic analysis and conserved motifs analysis, while gene analysis included chromosome localization and gene replication analysis, gene structure analysis, cis-regulatory element analysis and gene expression pattern analysis under two different stresses. A total of 299 candidate ZoMYB genes were discovered in Z. officinale. Based on the grouping of R2R3-MYB genes in a model plant Arabidopsis thaliana, ZoMYBs was divided into 8 groups and distributed across 22 chromosomes at uneven densities. Through gene duplication analysis, a total of 28 segmental duplication were identified in ginger genome. In addition, the expression pattern of 10 chosen ZoMYBs in leaves of Z. officinale were detected under ABA and low temperature stress treatments. The result revealed that there were differences in gene expression of these ten MYB genes in ginger under ABA treatment and low temperature treatment.Conclusions: Our results revealed the characteristics of ZoMYB genes family and provided valuable information for improving the ability of Z. officinale to cope with environmental changes during growth and development.
Investigating The Role of Households’ Health Care Expenditures on The Iranian Households’ Welfare Using DSGE Framework
Authors: Izadi Hamid Reza 
Published: May 2022

Abstract
Nowadays, the study of the share of households' health expenditures in their total consumption expenditures and its impact on the well-being of households and society has become an important issue as it is applied in policy and planning by government officials. The objective of this paper is to examine the impact of changes in the share of health expenditures in the basket of total household consumption expenditures and to show that small changes in the variable have significant effects on consumption, desirability, and consequently the well-being of society. This paper examines the role of households’ health care expenditures on the Iranian households’ welfare Using dynamic stochastic general equilibrium framework. The results of this modeling suggest that a decrease in the share of household health expenditures leads to an increase in other household expenditures and, consequently, increases household welfare by increasing utility. The reason for this is that by increasing its health and medical expenditures, the household must reduce its consumption of other goods, which decreases its total utility. It should be noted that this decrease is due to the fact that households are less inclined to spend a larger share of their total consumption expenditure on health services. In general, then, it can be said that an increase in the share of households' health expenditures in their total expenditures leads to a decrease in welfare, and a decrease in this share increases household welfare. However, factors such as the inefficiency of the insurance system, poor monitoring of the health system, and problems in accessing and using health services can have a major impact on households' acceptance, desire, and use of health services and should be considered a serious problem. JEL classification: E63, F42, H51, N3, p36
Early-life stress and dietary fatty acids impact the brain lipid/oxylipin profile into adulthood, basally and in response to LPS
Authors: Reemst Kitty , Broos Jelle Y. , Abbink Maralinde R. , Cimetti Chiara , Giera Martin , Kooij Gijs , Korosi Aniko 
Published: May 2022

Abstract
Brain lipid dysregulation is a hallmark of depression and Alzheimer’s disease, also marked by chronic inflammation. Early-life stress (ELS) and dietary intake of polyunsaturated fatty acids (PUFAs) are risk factors for these pathologies and are known to impact inflammatory processes. However, if these early-life factors alter brain lipid homeostasis on the long-term and thereby contribute to this risk remains to be elucidated. We have recently shown that an early diet enriched in omega(ω)-3 PUFAs protected against the long-term negative effects of ELS on cognition and neuroinflammation. Here, we aim to understand if modulation of brain lipid and oxylipin profiles contributes to the detrimental effects of ELS and the protective ones of the diet. We therefore studied if and how ELS and early dietary PUFAs modulate the brain lipid and oxylipin profile, basally as well as in response to an inflammatory challenge, to unmask possible latent effects. Male mice were exposed to ELS via the limited bedding and nesting paradigm, received an early diet with high or low ω6/ω3 ratio (HRD and LRD) and were injected with saline or lipopolysaccharide (LPS) in adulthood. Twenty-four hours later plasma cytokines (Multiplex) and hypothalamic lipids and oxylipins (liquid chromatography tandem mass spectrometry) were measured. ELS exacerbated the LPS-induced increase in IL-6, CXCL1 and CCL2. Both ELS and diet affected the lipid/oxylipin profile long-term. For example, ELS increased diacylglycerol and LRD reduced triacylglycerol, free fatty acids and ceramides. Importantly, the ELS-induced alterations were strongly influenced by the early diet. For example, the ELS-induced decrease in eicosapentaenoic acid was reversed when fed LRD. Similarly, the majority of the LPS-induced alterations were distinct for control and ELS exposed mice and unique for mice fed with LRD or HRD. LPS decreased ceramides and lysophosphotidylcholine, increased hexosylceramides and prostaglandin E2, reduced triacylglycerol species and ω6-derived oxylipins only in mice fed LRD and ELS reduced the LPS-induced increase in phosphatidylcholine. These data give further insights into the alterations in brain lipids and oxylipins that might contribute to the detrimental effects of ELS, to the protective ones of LRD and the possible early-origin of brain lipid dyshomeostasis characterizing ELS-related psychopathologies.
Characterization of an endoplasmic reticulum stress-related signature to predict prognosis in Colorectal Adenocarcinoma
Authors: Xu Yu , Xie Yang-Mei , Sun Wen-Sha , Zi Rong , Lu Hong-Qiao , Xiao Le , Guo Shi-Kui , Gong Kun-Mei 
Published: May 2022

Abstract
Background
Colorectal cancer (CRC) is a malignant tumor of the digestive tract with high incidence and mortality rates. Colorectal adenocarcinoma (COAD) is accounting for the majority of CRC. Endoplasmic reticulum stress-related genes play a role in cancer. This study aimed to develop a novel prognostic risk model for COAD based on endoplasmic reticulum stress-related genes, providing theoretical support for prognosis and clinical treatment ofCOAD.
Methods
Two cohorts, The cancer genome atlas (TCGA)- COAD cohort and GSE39582 dataset were enrolled in the study. The differentially expressed genes (DEGs) were determined using the ‘limma’ R package. The ‘clusterProfiler’ R package was utilized to conduct Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Univariate and multivariate Cox analyses were performed to identify the prognostic genes. The risk score model was constructed and verified by internal and external cohorts. We analyzed the changes of enriched pathways in the high- and low-risk groups by gene set variation analysis(GSVA). The immune infiltration landscape was calculated by single sample gene set enrichment analysis (ssGSEA) and the immunotherapy response was assessed by the Tumour Immune Dysfunction and Exclusion (TIDE) algorithm. The expression of prognostic genes in clinically normal and COAD samples was verified by Real Time Quantitative polymerase chain reaction (RT-qPCR).
Results
A total of 147 differentially expressed endoplasmic reticulum stress-related genes (DE-ERSGs) in COAD were identified. We screened eight prognostic genes to construct endoplasmic reticulum stress-related gene signature through Cox regression analysis in the training set of TCGA-COAD cohort. Then we validated the risk score model by a testing set of TCGA-COAD cohort and GSE39582 cohort. The univariate and multivariate Cox regression analysis demonstrated the risk score was a robust independent prognostic factor in overall survival prediction. The GSVA also suggested that the gene signature was related to the immune-related pathways. The result of ssGSEA implied that there were significant differences in the immune microenvironment between the high- and low-risk groups. The TIDE analysis revealed that the risk score could predict the clinical response of immunotherapy. The result of RT-qPCR demonstrated that the expression trends of prognostic genes in clinical samples were consistent with the result from public database.
Conclusion
Our study identified prognostic endoplasmic reticulum stress-related genes and established a novel risk signature, which could predict prognosis in COAD patients and provide theoretical support for clinical treatment of COAD.
Waste haven effect: unpacking the impact of environmental regulation
Authors: Rocha Thais Nunez 
Published: May 2022

Abstract
Developing countries can become waste havens due to environmental regulation differences with their trade partners. This paper analyses the effectiveness of the implementation of the Basel Convention in the European Union (EU-WSR), by studying the impact of the EU-WSR on hazardous waste trade. The analysis focuses first on the less developed EU countries, and then on developing countries. To that end, a gravity model is applied to a panel dataset. Results show that there is not enough evidence to identify a waste haven effect in the less developed EU countries, using both aggregated and disaggregated measures of environmental regulations. Increasing differences in the ratification of environmental treaties could lead to growing imports of waste though. In the analysis for developing countries, there is no evidence of the efficacy of the EU-WSR. Moreover, it is found that increasing differences in environmental regulation can lead to a Waste Haven Effect. These findings provide insights into the efficacy of International Environmental Agreements.JEL codes: F13, F18, Q53, Q56, Q58
Towards the Ionizing Radiation Induced Bond Dissociation Mechanism in Oxygen, Water, Guanine and DNA Fragmentation: A Density Functional Theory Simulation
Authors: KC Santosh , Abolfath Ramin 
Published: May 2022

Abstract
The radiation-induced damages in bio-molecules are ubiquitous processes in radiotherapy and radio-biology, and critical to space projects. In this study, we present a precise quantification of the fragmentation mechanisms of deoxyribonucleic acid (DNA) and the molecules surrounding DNA such as oxygen and water under non-equilibrium conditions using the first-principle calculations based on density functional theory (DFT). Our results reveal the structural stability of DNA bases and backbone that withstand up to a combined threshold of charge and hydrogen abstraction owing to simultaneously direct and indirect ionization processes. We show the hydrogen contents of the molecules significantly control the stability in the presence of radiation. This study provides comprehensive information on the impact of the direct and indirect induced bond dissociations and DNA damage and introduces a systematic methodology for fine-tuning the input parameters necessary for the large-scale Monte Carlo simulations of radio-biological responses and mitigation of detrimental effects of ionizing radiation.
Microalgal potential for sustainable biodiesel production and other bioactives compounds
Authors: Bajwa Kulvinder , Bishnoi Narsi R. , Baloch Muhammad Yousuf Jat , Kumar S. P. Jeevan , Selven Silambrasan Tamil 
Published: May 2022

Abstract
Microalgae have been gaining wide attention from scientific and industrial point of view as a source of high-lipid feedstock to produce biodiesel. Current research work was focused primarily on biodiesel production using algal biomass with the objectives of collection, isolation, screening and characterization of appropriate algal species. In this context, sixty seven microalgal strains were isolated including fresh and marine water sources from five regions of India. Screening of high lipid microalgae strains was carried out using Nile Red method for detection of neutral lipid droplets. Out of sixty seven, four potent biodiesel producing strains namely Chlorococcum aquaticum, Scenedesmus obliquus, Nannochloropsis oculata and Chlorella pyrenoidosa were selected on the basis of high lipid and biomass accumulation and having desirable FAME profile of palmitic (16:0), palmitoleic (C16:1), stearic (C-18:0) and oleic acids (C-18:1), respectively, which implies that these strains can be successfully used for biodiesel production.
Digital Holographic Microscopy of Spiropyran-Based Dynamic Materials
Authors: Jamali Ramin , Rad Vahideh Farzam , Razaghi Masoumeh , Mohamadnia Zahra , Khorasani Mojtaba , Moradi Ali-Reza 
Published: May 2022

Abstract
Spiropyran (SP) based dynamic materials undergo structural changes in response to external stimuli. In this paper, we show that digital holographic microscopy (DHM) is an effective candidate for characterization of SPs (embedded in polymer matrices) and for monitoring of their dynamical changes. The polymer matrices are polylactic acid (PLA) and poly(methyl methacrylate) (PMMA) films, which are decorated with SPs and immobilized on graphene quantum dots (GQDs). GQDs are modified by benzylamines prior to the loading of SP species because of the enhancement of hydrophobic characteristics. UV irradiation is used as the external stimulus and the dynamical changes of the samples before and after UV irradiation are measured. DHM is arranged on a novel self-referencing setup which substantially reduces the sensitivity of DHM to environmental vibrations. Morphometric information for characterization of the samples are obtained by analysis of the recorded digital holograms. The experimental results demonstrate the potential of the presented technique to serve as an alternative technique for surface measurement methodologies such as atomic force microscope and stylus profiler for surface characterization of similar materials.
SGLT2 inhibitor versus carbohydrate-restricted isocaloric diet: Reprogramming oxidation in type 2 diabetes
Authors: Igarashi Hiroyuki , Uchino Hiroshi , Kanaguchi Momoko , Hisanaga Kaori , Sato Genki , Yoshikawa Fukumi , Furuta Masashi , Washizawa Naohiro , Usui Shuki , Miyagi Masahiko , Hirose Takahisa 
Published: May 2022

Abstract
Objective: This study aimed to determine whether the carbohydrate-restricted isocaloric (CRIC) diet and sodium/glucose cotransporter-2 inhibitor (SGLT2i) differed with respect to the fasting and postprandial energy expenditure (EE) and macronutrient oxidation, and insulin dose required to achieve euglycemia. Methods: This randomized 3-arm open-label prospective study enrolled 23 patients with type 2 diabetes (male/female: 14/9, age: 53.6±14.2 years, body mass index: 26.9±4.8 kg/m2, HbA1c: 12.5±1.6%) treated with titrated basal-bolus insulin subsequent to the control diet (CON) alone, administration of canagliflozin 100 mg/day (SGLT2i) to the CON, or CRIC diet for 12 weeks. The main outcome measures included the fasting and postprandial EE, respiratory quotient (RQ), and required dose of insulin. Results: Although the fasting EE was similar in all groups, postprandial EE was significantly elevated in the SGLT2i and CRIC groups compared to the CON group (p=0.03 and 0.04). The fasting RQ was lower in the SGLT2i group (p=0.049) and delta-RQ (postprandial RQ/fasting RQ) was elevated due to increased carbohydrate substrate utilization in the SGLT2i (p=0.04) and CRIC (p=0.03) groups compared to the CON group. The total required daily dose of insulin was significantly higher in the CRIC (p=0.02) group, although the amount of available carbohydrate was similar in the CRIC and SGLT2i groups. Conclusion: The CRIC diet resulted in a similar fasting and postprandial EE and substrate oxidation compared to SGLT2i. The increased insulin requirement in the CRIC diet indicates that a relatively high lipid and protein ratio (compared to the SGLT2i and CON) may influence insulin requirement.