bluuebunny/crossref_metadata_2025_split huggingface dataset:
Guided Wave Transceive Technique Using Magnetostrictive Effect of STPG370 Pipe itself and High-Temperature Superconductor SQUID
Authors: Watanabe K , Okada K , Masumitsu S , Munkhnyam T , Sun W , Hatsukade Y , Shoyama Y , Tomotoshi A 
Published: August 2022

Abstract
We developed a non-contacting ultrasonic guided wave testing technique using magnetostrictive effects and high-temperature superconductor (HTS) SQUID for STPG370 steel pipes. A pair of C-shaped electromagnets was used to magnetize the STPG370 pipe while rotating the pipe, in order to give uniform remnant fields as bias fields. Conditions to give larger remnant field in the pipe were investigated using an electromagnetic field simulation. The simulation results indicated that C-shaped pb permalloy iron cores with an aperture angle of 20 degree and a diameter of 20 mm should be utilized for the magnetization. An excitation coil was wound around one of the magnetized parts, which is used as a transmitter. One cycle sine current of 9 App at 50 kHz was repeatedly supplied to the coil to generate T(0,1) mode guided waves in the pipe. The other magnetized part, which was used as a receiver, was combined with the HTS-SQUID gradiometer, which was located above the pipe with a lift-off of 4 mm. In measurements, the pipe was rotated to perform all-round measurements. A contour map of the time-series signal distribution of T(0, 1) mode guided wave was obtained around the pipe.
Neutron Imaging toward Epithermal Regime using a Delay Line Current-Biased Kinetic-Inductance Detector
Authors: Shishido Hiroaki , Vu The Dang , Aizawa Kazuya , Kojima Kenji M. , Koyama Tomio , Oikawa Kenichi , Harada Masahide , Oku Takayuki , Soyama Kazuhiko , Miyajima Shigeyuki , Hidaka Mutsuo , Suzuki Soh Y. , Tanaka Manobu M. , Kawamata Shuichi , Ishida Takekazu 
Published: August 2022

Abstract
Wide energy range neutron imaging and spectroscopy in a single experiment without changing the setup is available by a combination of a high-power pulsed neutron source and fast operating neutron detector. The delay-line current-biased kinetic-inductance detector (CB-KID) is a two-dimensional superconducting detector with high spatial and temporal resolutions, and multi-hit tolerance. We demonstrate that the delay-line CB-KID with 10B neutron conversion layer is applicable for neutron imaging and spectroscopy up to the neutron energy of 100 eV. High spatial neutron transmission image for a Sm-Sn mixed metal is successfully constructed below 0.3 eV. Concomitantly, we examine the neutron transmission up to 100 eV and identify observed resonance dips by Sm nuclei.
Design and fabrication of microwave transmitting antenna using HTS thick disk for wireless power transfer
Authors: Saito A , Shimada F , Sato T , Kiyooka K , Shibata M , Ono S , Takeda M , Narita Y , Nakajima K 
Published: August 2022

Abstract
We designed and fabricated a microwave transmitting antenna that uses an HTS thick disk as a patch antenna with a resonant frequency of around 5.0 GHz. The reflection property S
11, electromagnetic fields, current distributions, and far-field of several resonant modes in the antenna were analyzed using the CST studio suite. We compared the properties in several resonance modes and selected the TM11 mode, which has the smallest resonator size and the highest directivity. The antenna was assembled using a GdBCO thick disk, sapphire substrates, Cu housing, and SMA connector. We measured the S
11 using a vector network analyzer and pulse tube refrigerator and found that it had a resonant frequency of 4.996 GHz and a Q
u of 405 at the temperature of 48 K. These measured properties were in good agreement with the simulated results.
A deep learning method combined with electronic nose to identify the rice origin
Authors: Yan Chaohua , Lu An 
Published: August 2022

Abstract
The rice quality is directly affected by the natural environment in which it is grown. It is common to pass off poor quality as good quality rice sold in rice markets. Therefore, an effective rice quality detection method should be proposed. This work proposes a deep learning method combined with an electronic nose (e-nose) to realize the classification of rice origin, and then realize the detection of rice quality. Firstly, channel-spatial collaborative attention (CSCA) is proposed to adaptively focus on key features of gas information. Secondly, three shallow convolutional neural network (CNN) structures are introduced to achieve gas information classification. Finally, CSCA and three CNN structures are combined to construct a classification network for rice gas recognition. The results show that in the comparison of multi-structure models, LeNet5-CSCA has the best classification performance, with a classification accuracy of 97.70%, a precision of 97.45%, a recall of 97.67%, and a F1-score of 97.56%.
Superlarge living hyaline cartilage graft contributed by the scale-changed porous 3D culture system for joint defect repair
Authors: Yao Hang , Li Tianliang , Wu Zhonglian , Tao Qi , Shi Junli , Liu Lihua , Zhao Yuchi 
Published: August 2022

Abstract
It is known that an excellent hyaline cartilage phenotype, an internal microstructure with safe crosslinking and available size flexibility are the key factors of cartilage grafts that allow for clinical application. Living hyaline cartilage grafts (LhCGs) constructed by phase-transfer hydrogel (PTCC) systems were reported to have a hyaline phenotype and bionic microstructure. By employing chondrocytes to secrete matrix in the hydrogel and then removing the material to obtain material-free tissue in vitro, LhCG technology exhibited superior performance in cartilage repair. However, PTCC systems could only produce small-sized LhCGs because of medium delivery limitations, which hinders the clinical application of LhCGs. In this study, we prepared three different noncrosslinked gelatin microspheres with diameters from 200 μm to 500 μm, which replaced the original pore-forming agent. The new PTCC system with the mixed and gradient porous structure was used for the preparation of superlarge LhCGs with a continuous structure and hyaline phenotype. Compared to the original technique, the porous gradient structure promoted nutrient delivery and cartilage matrix secretion. The small size of the microporous structure promoted the rapid formation of matrix junctions. The experimental group with a mixed gradient increased cartilage matrix secretion significantly by more than 50% compared to the that of the control. The LhCG final area reached 7 cm2 without obvious matrix stratification in the mixed gradient group. The design of the scale-changed porous PTCC system will make LhCGs more promising for clinical application.
N = 2 quantum chiral superfields and quantum super bundles
Authors: Fioresi R , Lledó M A , Razzaq J 
Published: August 2022

Abstract
We give the superalgebra of N = 2 chiral (and antichiral) quantum superfields realized as a subalgebra of the quantum supergroup SL
q
(4|2). The multiplication law in the quantum supergroup induces a coaction on the set of chiral superfields. We also realize the quantum deformation of the chiral Minkowski superspace as a quantum principal bundle.
Stitching and registering highly multiplexed whole-slide images of tissues and tumors using ASHLAR
Authors: Muhlich Jeremy L , Chen Yu-An , Yapp Clarence , Russell Douglas , Santagata Sandro , Sorger Peter K 
Published: August 2022

AbstractMotivationStitching microscope images into a mosaic is an essential step in the analysis and visualization of large biological specimens, particularly human and animal tissues. Recent approaches to highly multiplexed imaging generate high-plex data from sequential rounds of lower-plex imaging. These multiplexed imaging methods promise to yield precise molecular single-cell data and information on cellular neighborhoods and tissue architecture. However, attaining mosaic images with single-cell accuracy requires robust image stitching and image registration capabilities that are not met by existing methods.ResultsWe describe the development and testing of ASHLAR, a Python tool for coordinated stitching and registration of 103 or more individual multiplexed images to generate accurate whole-slide mosaics. ASHLAR reads image formats from most commercial microscopes and slide scanners, and we show that it performs better than existing open-source and commercial software. ASHLAR outputs standard OME-TIFF images that are ready for analysis by other open-source tools and recently developed image analysis pipelines.Availability and implementationASHLAR is written in Python and is available under the MIT license at https://github.com/labsyspharm/ashlar. The newly published data underlying this article are available in Sage Synapse at https://dx.doi.org/10.7303/syn25826362; the availability of other previously published data re-analyzed in this article is described in Supplementary Table S4. An informational website with user guides and test data is available at https://labsyspharm.github.io/ashlar/.Supplementary informationSupplementary data are available at Bioinformatics online.
‘People are Trapped in History and History is Trapped Inside Them’: Exploring Britain’s Racialized Colonial Legacies in Criminological Research
Authors: Parmar Alpa , Earle Rod , Phillips Coretta 
Published: August 2022

AbstractIn this paper, we advocate identifying the colonizing logics of race in criminological analysis, in recognition of their enduring postcolonial presence in societies like the United Kingdom. Our argument unfolds through the life stories narrated by three men entangled by colonial remnants of power(lessness), subservience, and criminalization. For Cairo (black) and Rafan (Asian), their subalternity is exposed through their vulnerability to racialized stereotypes which have a foundation in colonial histories. Both young minority ethnic men are also situated in a transnational, glocalized frame in which their racialized and gendered identities prescribed the nature of their belonging to a British nation-state irrevocably connected to the Empire. Our reflections on a third narrative interview of Barry reveal the dividends of whiteness, simultaneously occluded, disavowed, and yet wilfully upheld, but in a competitive and exclusive form of belonging. In the articulation and analysis of these stories of crime and order, we mine styles of reasoning from postcolonial theory, sociology, history, and philosophy to embolden a postcolonial criminology.
Alcohol use in adolescence as a risk factor for overdose in the 1986 Northern Finland Birth Cohort Study
Authors: Koivisto Maarit K , Miettunen Jouko , Levola Jonna , Mustonen Antti , Alakokkare Anni-Emilia , Salom Caroline L , Niemelä Solja 
Published: August 2022

Abstract

Background
Overdoses and poisonings are among the most common causes of death in young adults. Adolescent problem drinking has been associated with psychiatric morbidity in young adulthood as well as with elevated risk for suicide attempts. There is limited knowledge on adolescent alcohol use as a risk factor for alcohol and/or drug overdoses in later life.

Methods
Here, data from The Northern Finland Birth Cohort 1986 study with a follow-up from adolescence to early adulthood were used to assess the associations between adolescent alcohol use and subsequent alcohol or drug overdose. Three predictors were used: age of first intoxication, self-reported alcohol tolerance and frequency of alcohol intoxication in adolescence. ICD-10-coded overdose diagnoses were obtained from nationwide registers. Use of illicit drugs or misuse of medication, Youth Self Report total score, family structure and mother’s education in adolescence were used as covariates.

Results
In multivariate analyses, early age of first alcohol intoxication [hazard ratios (HR) 4.5, 95% confidence intervals (CI) 2.2–9.2, P &lt; 0.001], high alcohol tolerance (HR 3.1, 95% CI 1.6–6.0, P = 0.001) and frequent alcohol intoxication (HR 1.9, 95% CI 1.0–3.4, P = 0.035) all associated with the risk of overdoses. Early age of first intoxication (HR 5.2, 95% CI 1.9–14.7, P = 0.002) and high alcohol tolerance (HR 4.4, 95% CI 1.7–11.5, P = 0.002) also associated with intentional overdoses.

Conclusions
Alcohol use in adolescence associated prospectively with increased risk of overdose in later life. Early age of first intoxication, high alcohol tolerance and frequent alcohol intoxication are all predictors of overdoses.
Co-administration of ursodeoxycholic acid with rosuvastatin/ezetimibe in a non-alcoholic fatty liver disease model
Authors: Seo Sang Hyun , Lee Da Hyun , Lee Yu Seol , Cho Kyung Joo , Park Hye Jung , Lee Hye Won , Kim Beom Kyung , Park Jun Yong , Kim Do Young , Ahn Sang Hoon , Bae Soo Han , Kim Seung Up 
Published: August 2022

AbstractBackgroundUrsodeoxycholic acid (UDCA), statins, and ezetimibe (EZE) have demonstrated beneficial effects against non-alcoholic fatty liver disease (NAFLD). We investigated the efficacy of the combination of UDCA and the mix of rosuvastatin (RSV)/EZE in the treatment of NAFLD.MethodsNAFLD mouse models were developed by injecting thioacetamide, fasting, and high-carbohydrate refeeding, high-fat diet, and choline-deficient L-amino acid-defined high-fat diet (CDAHFD). Low-dose UDCA (L-UDCA; 15 mg/kg) or high-dose UDCA (H-UDCA; 30 mg/kg) was administered with RSV/EZE. We also employed an in vitro model of NAFLD developed using palmitic acid-treated Hepa1c1c7 cells.ResultsCo-administration of RSV/EZE with UDCA significantly decreased the collagen accumulation, serum alanine aminotransferase (ALT) levels, and mRNA levels of fibrosis-related markers than those observed in the vehicle group in thioacetamide-treated mice (all P &lt; 0.01). In addition, in the group fasted and refed with a high-carbohydrate diet, UDCA/RSV/EZE treatment decreased the number of apoptotic cells and serum ALT levels compared with those observed in the vehicle group (all P &lt; 0.05). Subsequently, H-UDCA/RSV/EZE treatment decreased the number of ballooned hepatocytes and stearoyl-CoA desaturase 1 (SCD-1) mRNA levels (P = 0.027) in the liver of high-fat diet-fed mice compared with those observed in the vehicle group. In the CDAHFD-fed mouse model, UDCA/RSV/EZE significantly attenuated collagen accumulation and fibrosis-related markers compared to those observed in the vehicle group (all P &lt; 0.05). In addition, UDCA/RSV/EZE treatment significantly restored cell survival and decreased the protein levels of apoptosis-related markers compared to RSV/EZE treatment in palmitic acid-treated Hepa1c1c7 cells (all P &lt; 0.05).ConclusionCombination therapy involving UDCA and RSV/EZE may be a novel strategy for potent inhibition of NAFLD progression.
Faecal carriage of enterococci harbouring oxazolidinone resistance genes among healthy humans in the community in Switzerland
Authors: Nüesch-Inderbinen Magdalena , Biggel Michael , Zurfluh Katrin , Treier Andrea , Stephan Roger 
Published: August 2022

Abstract

Objectives
This study aimed to investigate the faecal carriage of enterococci harbouring oxazolidinone resistance genes among healthy humans in Switzerland and to genetically characterize the isolates.

Methods
A total of 399 stool samples from healthy individuals employed in different food-processing plants were cultured on a selective medium containing 10 mg/L florfenicol. Resulting enterococci were screened by PCR for the presence of cfr, optrA and poxtA. A hybrid approach combining short-read and long-read WGS was used to analyse the genetic context of the cfr, optrA and poxtA genes.

Results
Enterococcus faecalis (n = 6), Enterococcus faecium (n = 6), Enterococcus gallinarum (n = 1) and Enterococcus hirae (n = 2) were detected in 15/399 (3.8%) of the faecal samples. They carried cfr + poxtA, optrA, optrA + poxtA or poxtA. Four E. faecalis harbouring optrA and one E. faecium carrying poxtA were resistant to linezolid (8 mg/L). In most optrA-positive isolates, the genetic environments of optrA were highly variable, but often resembled previously described platforms. In most poxtA-positive isolates, the poxtA gene was flanked on both sides by IS1216E elements and located on medium-sized plasmids.

Conclusions
Faecal carriage of Enterococcus spp. harbouring cfr, optrA and poxtA in healthy humans associated with the food-production industry demonstrates the possibility of spread of oxazolidinone resistance genes into the community. Given the importance of linezolid as a last-resort antibiotic for the treatment of serious infections caused by Gram-positive pathogens, the detection of the oxazolidinone resistance determinants in enterococci from healthy humans is of concern for public health.
A natural product from Streptomyces targets PhoP and exerts antivirulence action against Salmonella enterica
Authors: Bruna Roberto E , Casal Alejo , Bercovich Bárbara , Gramajo Hugo , Rodríguez Eduardo , García Véscovi Eleonora 
Published: August 2022

Abstract

Background
The overprescription and misuse of classical antimicrobial compounds to treat gastrointestinal or systemic salmonellosis have been accelerating the surge of antibiotic-recalcitrant bacterial populations, posing a major public health challenge. Therefore, alternative therapeutic approaches to treat Salmonella infections are urgently required.

Objectives
To identify and characterize actinobacterial secreted compounds with inhibitory properties against the Salmonella enterica PhoP/PhoQ signal transduction system, crucial for virulence regulation.

Methods
The methodology was based on a combination of the measurement of the activity of PhoP/PhoQ-dependent and -independent reporter genes and bioguided assays to screen for bioactive inhibitory metabolites present in culture supernatants obtained from a collection of actinobacterial isolates. Analogues of azomycin were used to analyse the functional groups required for the detected bioactivity and Salmonella mutants and complemented strains helped to dissect the azomycin mechanism of action. The tetrazolium dye colorimetric assay was used to investigate azomycin potential cytotoxicity on cultured macrophages. Salmonella intramacrophage replication capacity upon azomycin treatment was assessed using the gentamicin protection assay.

Results
Sublethal concentrations of azomycin, a nitroheterocyclic compound naturally produced by Streptomyces eurocidicus, repressed the Salmonella PhoP/PhoQ system activity by targeting PhoP and inhibiting its transcriptional activity in a PhoQ- and aspartate phosphorylation-independent manner. Sublethal, non-cytotoxic concentrations of azomycin prevented Salmonella intramacrophage replication.

Conclusions
Azomycin selectively inhibits the activity of the Salmonella virulence regulator PhoP, a new activity described for this nitroheterocyclic compound that can be repurposed to develop novel anti-Salmonella therapeutic approaches.
GAIA: G-quadruplexes in alive creature database
Authors: Vannutelli Anaïs , Schell Lauriane Lucienne Noele , Perreault Jean-Pierre , Ouangraoua Aïda 
Published: August 2022

Abstract
G-quadruplexes (G4) are 3D structures that are found in both DNA and RNA. Interest in this structure has grown over the past few years due to both its implication in diverse biological mechanisms and its potential use as a therapeutic target, to name two examples. G4s in humans have been widely studied; however, the level of their study in other species remains relatively minimal. That said, progress in this field has resulted in the prediction of G4s structures in various species, ranging from bacteria to eukaryotes. These predictions were analysed in a previous study which revealed that G4s are present in all living kingdoms. To date, eleven different databases have grouped the various G4s depending on either their structures, on the proteins that might bind them, or on their location in the various genomes. However, none of these databases contains information on their location in the transcriptome of many of the implicated species. The GAIA database was designed so as to make this data available online in a user-friendly manner. Through its web interface, users can query GAIA to filter G4s, which, we hope, will help the research in this field. GAIA is available at: https://gaia.cobius.usherbrooke.ca
IDH1/2wildtype gliomas grade 2 and 3 with molecular glioblastoma-like profile have a distinct course of epilepsy compared toIDH1/2wildtype glioblastomas
Authors: van Opijnen Mark P , Tesileanu C Mircea S , Dirven Linda , van der Meer Pim B , Wijnenga Maarten M J , Vincent Arnaud J P E , Broekman Marike L D , Dubbink Hendrikus J , Kros Johan M , van Duinen Sjoerd G , Smits Marion , French Pim J , van den Bent Martin J , Taphoorn Martin J B , Koekkoek Johan A F 
Published: August 2022

AbstractBackgroundIDH1/2 wildtype (IDHwt) glioma WHO grade 2 and 3 patients with pTERT mutation and/or EGFR amplification and/or + 7/−10 chromosome gain/loss have a similar overall survival time as IDHwt glioblastoma patients, and are both considered glioblastoma IDHwt according to the WHO 2021 classification. However, differences in seizure onset have been observed. This study aimed to compare the course of epilepsy in the 2 glioblastoma subtypes.MethodsWe analyzed epilepsy data of an existing cohort including IDHwt histologically lower-grade glioma WHO grade 2 and 3 with molecular glioblastoma-like profile (IDHwt hLGG) and IDHwt glioblastoma patients. Primary outcome was the incidence proportion of epilepsy during the disease course. Secondary outcomes included, among others, onset of epilepsy, number of seizure days, and antiepileptic drug (AED) polytherapy.ResultsOut of 254 patients, 78% (50/64) IDHwt hLGG and 68% (129/190) IDHwt glioblastoma patients developed epilepsy during the disease (P = .121). Epilepsy onset before histopathological diagnosis occurred more frequently in IDHwt hLGG compared to IDHwt glioblastoma patients (90% vs 60%, P &lt; .001), with a significantly longer median time to diagnosis (3.5 vs 1.3 months, P &lt; .001). Median total seizure days was also longer for IDHwt hLGG patients (7.0 vs 3.0, P = .005), and they received more often AED polytherapy (32% vs 17%, P = .028).ConclusionsAlthough the incidence proportion of epilepsy during the entire disease course is similar, IDHwt hLGG patients show a significantly higher incidence of epilepsy before diagnosis and a significantly longer median time between first seizure and diagnosis compared to IDHwt glioblastoma patients, indicating a distinct clinical course.
Glutamate 1-semialdehyde aminotransferase is connected to GluTR by GluTR-binding protein and contributes to the rate-limiting step of 5-aminolevulinic acid synthesis
Authors: Sinha Neha , Eirich Jürgen , Finkemeier Iris , Grimm Bernhard 
Published: August 2022

AbstractTetrapyrroles play fundamental roles in crucial processes including photosynthesis, respiration, and catalysis. In plants, 5-aminolevulinic acid (ALA) is the common precursor of tetrapyrroles. ALA is synthesized from activated glutamate by the enzymes glutamyl-tRNA reductase (GluTR) and glutamate-1-semialdehyde aminotransferase (GSAAT). ALA synthesis is recognized as the rate-limiting step in this pathway. We aimed to explore the contribution of GSAAT to the control of ALA synthesis and the formation of a protein complex with GluTR. In Arabidopsis thaliana, two genes encode GSAAT isoforms: GSA1 and GSA2. A comparison of two GSA knockout mutants with the wild-type revealed the correlation of reduced GSAAT activity and ALA-synthesizing capacity in leaves with lower chlorophyll content. Growth and green pigmentation were more severely impaired in gsa2 than in gsa1, indicating the predominant role of GSAAT2 in ALA synthesis. Interestingly, GluTR accumulated to higher levels in gsa2 than in the wild-type and was mainly associated with the plastid membrane. We propose that the GSAAT content modulates the amount of soluble GluTR available for ALA synthesis. Several different biochemical approaches revealed the GSAAT–GluTR interaction through the assistance of GluTR-binding protein (GBP). A modeled structure of the tripartite protein complex indicated that GBP mediates the stable association of GluTR and GSAAT for adequate ALA synthesis.