bluuebunny/crossref_metadata_2025_split huggingface dataset:
Verbal fluency functional magnetic resonance imaging detects anti‐seizure effects and affective side effects of perampanel in people with focal epilepsy
Authors: Xiao Fenglai , Caciagli Lorenzo , Wandschneider Britta , Fleury Marine , Binding Lawrence , Giampiccolo Davide , Hill Andrea , Galovic Marian , Foong Jaqueline , Zhou Dong , Sander Josemir W. , Duncan John S. , Koepp Matthias J. 
Published: December 2022

AbstractPerampanel, a noncompetitive antagonist of the postsynaptic a‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic (AMPA) receptor, is effective for controlling focal to bilateral tonic–clonic seizures but is also known to increase feelings of anger. Using statistical parametric mapping–derived measures of activation and task‐modulated functional connectivity (psychophysiologic interaction), we investigated 14 people with focal epilepsy who had verbal fluency functional magnetic resonance imaging (fMRI) twice, before and after the add‐on treatment of perampanel. For comparison, we included 28 people with epilepsy, propensity‐matched for clinical characteristics, who had two scans but no change in anti‐seizure medication (ASM) regimen in‐between. After commencing perampanel, individuals had higher task‐related activations in left orbitofrontal cortex (OFC), fewer task‐related activations in the subcortical regions including the left thalamus and left caudate, and lower task‐related thalamocaudate and caudate‐subtantial nigra connectivity. Decreased task‐related connectivity is observed between the left OFC and precuneus and left medial frontal lobe. Our results highlight the brain regions associated with the beneficiary therapeutic effects on focal to bilateral tonic–clonic seizures (thalamus and caudate) but also the undesired affective side effects of perampanel with increased anger and aggression (OFC).
An epigenetic DNA methylation clock for age estimates in Indo‐Pacific bottlenose dolphins (Tursiops aduncus)
Authors: Peters Katharina J. , Gerber Livia , Scheu Luca , Cicciarella Riccardo , Zoller Joseph A. , Fei Zhe , Horvath Steve , Allen Simon J. , King Stephanie L. , Connor Richard C. , Rollins Lee Ann , Krützen Michael 
Published: December 2022

AbstractKnowledge of an animal's chronological age is crucial for understanding and predicting population demographics, survival and reproduction, but accurate age determination for many wild animals remains challenging. Previous methods to estimate age require invasive procedures, such as tooth extraction to analyse growth layers, which are difficult to carry out with large, mobile animals such as cetaceans. However, recent advances in epigenetic methods have opened new avenues for precise age determination. These ‘epigenetic clocks’ present a less invasive alternative and can provide age estimates with unprecedented accuracy. Here, we present a species‐specific epigenetic clock based on skin tissue samples for a population of Indo‐Pacific bottlenose dolphins (Tursiops aduncus) in Shark Bay, Western Australia. We measured methylation levels at 37,492 cytosine‐guanine sites (CpG sites) in 165 samples using the mammalian methylation array. Chronological age estimates with an accuracy of ±1 year were available for 68 animals as part of a long‐term behavioral study of this population. Using these samples with known age, we built an elastic net model with Leave‐One‐Out‐Cross‐Validation, which retained 43 CpG sites, providing an r = 0.86 and median absolute age error (MAE) = 2.1 years (5% of maximum age). This model was more accurate for our data than the previously published methylation clock based on skin samples of common bottlenose dolphins (T. truncatus: r = 0.83, MAE = 2.2) and the multi‐species odontocete methylation clock (r = 0.68, MAE = 6.8), highlighting that species‐specific clocks can have superior performance over those of multi‐species assemblages. We further developed an epigenetic sex estimator, predicting sex with 100% accuracy. As age and sex are critical parameters for the study of animal populations, this clock and sex estimator will provide a useful tool for extracting life history information from skin samples rather than long‐term observational data for free‐ranging Indo‐Pacific bottlenose dolphins worldwide.
The microbial community spatially varies during a Microcystis bloom event in Lake Kinneret
Authors: Schweitzer‐Natan Orna , Ofek‐Lalzar Maya , Sher Daniel , Sukenik Assaf 
Published: December 2022

Abstract

Cyanobacterial blooms in lakes frequently display high spatial heterogeneity, which may drive temporal changes in microbial community structure. Here we report on the spatial (horizontal and vertical) structure of the cyanobacteria community and of the co‐occurring heterotroph microbial community during a major lake‐wide bloom event at Lake Kinneret (Sea of Galilee, Israel).

We sampled the upper water layer (0, 1 and 5 m depths) at five locations across the lake, spatially representing north, south, east, west and centre of the lake, on two dates during winter 2016 when the Microcystis biomass was high. We aimed to characterise the spatial distribution of the bloom and the associated microbiota, as well as testing the extent to which variation in the structure of the microbial community could be associated with Microcystis biomass.

During the winter 2016 bloom, Microcystis biomass was spatially heterogeneous, but the Microcystis population structure was rather homogenous, dominated by a single genotype (ITS sequence variant). By contrast, the composition of the bacterial community, assessed using 16S rRNA gene sequencing, was spatially heterogeneous, differing between depths, sampling locations and time.

The largest difference in microbial community structure was between particle‐associated (PA) and free‐living (FL) fractions. The PA community was characterised by a relatively lower diversity, and dominated by Proteobacteria, whereas the FL community was significantly more diverse and dominated by members of Proteobacteria, Bacteroidetes and Actinobacteria. Variation in the microbial community was significantly correlated with geographical location and sampling depth. However, there was no clear relationship between the Microcystis biomass density (cells biovolume) and the structure of the microbial communities.

These results suggest that the structure of the bacterial community is partly decoupled from Microcystis population density, and potentially more affected by other environmental factors such as temperature and/or physicochemical conditions. Our study highlights the importance of spatial information on bloom composition and intensity in clarifying the factors affecting the progression of cyanobacterial harmful algal blooms and how this impacts their microbial counterparts.
How do clients with multiple problems and (in)formal caretakers coproduce integrated care and support? A longitudinal study on integrated care trajectories of clients with multiple problems
Authors: Reinhoudt‐den Boer Lieke , van Wijngaarden Jeroen , Huijsman Robbert 
Published: December 2022

AbstractIntroductionIntegrated care can create several advantages, such as better quality of care and better outcomes. These advantages apply especially to clients with multiple problems (CWMPs) who have multiple, interconnected needs that span health and social issues and require different health care (e.g., mental health care or addiction care), social care (e.g., social benefits) and welfare services at the same time. Integrated care is most often studied as a phenomenon taking place at the system, organizational, professional and clinical levels. Therefore, in many studies, clients seem to be implicitly conceptualized as passive recipients of care. Less research has been conducted on how clients and (in)formal caretakers coproduce integrated care.MethodsWe performed a longitudinal study to investigate how CWPMs and (in)formal caretakers coproduce integrated care. Data were collected among CWMPs and their (in)formal caretakers in Rotterdam, the Netherlands. CWMPs' care trajectories were followed for 1–1.5 years. CWMPs were interviewed three times at an interval of 6 months (T0, T1, T2). Informal caretakers were interviewed three times (T0, T1, T2), and formal caretakers of 16 clients were interviewed twice (T1, T2). Data in the municipal record systems about participating CWMPs were also included.ResultsOur study shows that the CWMPs' multidimensional needs, which should function as the organizing principle of integrated care, are rarely completely assessed at the start (first 6 weeks) of CWMPs' care trajectories. Important drivers behind this shortcoming are the urgent problems CWMPs enter the support trajectory with, their lack of trust in ‘the government’ and the complexity of their situations. We subsequently found two distinct types of cases. The highest level of integrated care is achieved when formal caretakers initiate an iterative process in which the CWMP's multidimensional needs are constantly further mapped out and interventions are attuned to this new information.ConclusionsOur study indicates that integrated care is the joint product of formal caretakers and CWMPs. Integrated care however does not come naturally when CWMPs are ‘put at the center’. Professionals need to play a leading role in engaging CWMPs to coproduce integrated care.Patient ContributionCWMPs and their (in)formal caretakers participated in this study via interviews and contributed with their experiences of the process.
Bacterial decontamination of toothbrushes by immersion in a mouthwash containing 0.05% chlorhexidine and 0.05% cetylpyridinium chloride: A randomized controlled trial
Authors: Àlvarez Gerard , Soler‐Ollé Agnès , Isabal Sergio , León Rubén , Blanc Vanessa 
Published: December 2022

AbstractObjectiveToothbrushes are colonized by microorganisms, implying a risk of infection. That risk can be reduced by decreasing the microbial contamination of the filaments. Therefore, this study aimed to determine the antiseptic efficacy of a 0.05% chlorhexidine + 0.05% cetylpyridinium chloride mouthwash on toothbrushes.MethodsA total of twelve toothbrushes used three times/day for 14 days by orally and systemically healthy people were randomly split into two groups, and their heads were immersed for 2 h in PBS (control) or Perio·Aid Active Control (treatment). The microorganisms were recovered, and their number was calculated by culture, quantitative PCR, and viability PCR. Statistical differences were first assessed with a two‐way mixed ANOVA and subsequently with Student's t‐test.ResultsThe results showed no statistical differences in the total number of cells for the treatment (mean ± CI95% of 7.27 ± 1.09 log10 bacteria/ml) and the control (7.62 ± 0.64 log10 bacteria/ml) groups, but a significantly lower number of live cells in the treatment group (4.58 ± 0.61 log10 viable bacteria/ml and 2.15 ± 1.42 log10 cfu/ml) than in the control group (6.49 ± 1.39 log10 viable bacteria/ml and 5.04 ± 0.93 log10 cfu/ml).ConclusionsBased on our findings, sanitization of toothbrushes with this mouthwash reduces the number of live microorganisms adhered to the filaments. Such decrease of the bacterial load could include bacteria from the oral cavity, from the environment, and from nearby toothbrushes since the quantification was not limited to any bacterial taxon.
Factors affecting the infection control behaviours of Korean dental hygienists in response to the coronavirus disease 2019 pandemic
Authors: Park Hye‐Rin , Min Ji‐Hyun 
Published: December 2022

AbstractObjectivesThis study aimed to identify the factors affecting the prevention of cross‐infection with coronavirus disease‐19 (COVID‐19) among Korean dental hygienists.MethodsThe level of knowledge about COVID‐19 (KNWCO), level of awareness of infection control (AWRIC), degree of performance for infection control (PRFIC), and organizational factors for infection control (OFWIC) of clinical dental hygienists in Korea to prevent COVID‐19 transmission were investigated. A self‐written online questionnaire was administered to dental hygienists currently working in dental clinics or hospitals, and the survey results of 263 participants were analysed. Pearson's correlation coefficients were calculated for relationships between the KNWCO, AWRIC, PRFIC, and OFWIC. Multiple linear regression analysis was performed to identify the factors affecting the PRFIC. Statistical significance was set at p < 0.05.ResultsKnowledge about COVID‐19, AWRIC, PRFIC, and OFWIC were significantly correlated with each other. OFWIC (β = 0.491, p < 0.001), followed by AWRIC (β = 0.428, p < 0.001) and KNWCO (β = 0.095, p = 0.013), had the greatest impact on PRFIC with regard to the prevention of COVID‐19 transmission.ConclusionsOrganizational factors for infection control and AWRIC should be strengthened to improve PRFIC and prevent COVID‐19 transmission during dental care.
Efficacy of a new sonic powered toothbrush versus a manual toothbrush in a young population. A randomized cross‐over clinical trial
Authors: Lucía Barallat , Léna Ben‐Kiran , Xavi Calvo , Paniagua Blanca , Pascual‐La Rocca Andrés 
Published: December 2022

AbstractObjectiveTo compare the effectiveness of dental plaque removal between a new sonic toothbrush and a manual toothbrush.MethodsIn total, 75 healthy dental students participated in this randomized double‐blind crossover clinical trial. Subjects were randomly assigned to one of the two groups (manual or sonic toothbrush) and underwent a single toothbrushing exercise. Seven days after, patients were asked to repeat the same procedure with the other toothbrush. Plaque scores were recorded before and after brushing.ResultsA significant mean reduction in the full‐mouth plaque index was observed after the use of both type of brushes: 46.2% ± 0.28% (p = 0.000) and 50.5% ± 0.24% (p = 0.000) for the manual and sonic toothbrush, respectively. However, there were no statistically significant differences between the two types of brushes (p = 0.277). Regarding plaque reduction on the distinct tooth surfaces, differences between the groups reached statistical significance in favour of the sonic toothbrush only on the buccal sites (p = 0.003).ConclusionsBoth devices lead to similar plaque score reduction after a single tooth brushing.
A note on changes in additive risky benefits and risky costs
Authors: Menegatti Mario 
Published: December 2022

AbstractWe introduce a distinction between additive risky benefits and additive risky costs, showing that it is relevant in determining decision maker choices in the presence of changes in risk. Results obtained show the specific role of the order of risk change when facing the two types of risk. Similarities and differences with the case of multiplicative risks are discussed. Moreover, the analysis is performed in two models studying respectively saving and self‐protection and provides new applied results for both, some with reference to background risks.
SETDB1: A perspective into immune cell function and cancer immunotherapy
Authors: Johnson Eleanor , Salari Kiarash , Yang Shujie 
Published: December 2022

AbstractOncogene SET Domain Bifurcated 1 (SETDB1)/ESET, an H3K9 methyltransferase, was originally discovered over two decades ago; however, its function in the immune response was not first reported until 2011. SETDB1 immune functions include B cell maturation, T cell activity regulation, and immune escape in cancer cells. In B lymphocytes, SETDB1 mediates the transition from pro‐B to pre‐B cells and represses endogenous retroviruses (ERV) to encourage B cell lineage differentiation and maturation. SETDB1 alters T cell function by methylating IL‐2 and IL‐17 promoters and mediating T cell lineage commitment and development. In addition, SETDB1 plays a critical role in ERV silencing within a variety of immune cells, which can indirectly weaken the immune response. Although SETDB1 is critical for normal immune cell function, overexpression in cancer cells negatively impacts immune cell fights against cancer through decreased tumour immunogenicity. Within cancer cells, SETDB1 overexpression represses production and infiltration of antitumour immune cells, mediates immune escape through TE and ERV silencing, represses the type I interferon pathway, and interferes in immune checkpoint blockade (ICB) outcomes by regulation of PD‐L1 expression and IFN signalling. In this review, we further discuss the immunological mechanisms of SETDB1 in normal and cancerous cells and its implications in cancer immunotherapy.
‘No more heroes’: The ILC Oxford Statement on fundamental care in times of crises
Authors: Kitson Alison L. , Conroy Tiffany , Jeffs Lianne , Carr Devin , Huisman‐Dewaal Getty J. , Muntlin Asa , Jangland Eva , Grønkjær Mette , Parr Jenny 
Published: December 2022

AbstractAimTo outline the International Learning Collaborative (ILC) Oxford Statement, explicating our commitment to ensuring health and care systems are equipped to meet patients' fundamental care needs during times of unprecedented crisis.Design/MethodDiscussion paper. The content was developed via a co‐design process with participants during the ILC's international conference.Key ArgumentsWe, the ILC, outline what we do and do not want to see within our health and care systems when faced with the challenges of caring for patients during global pandemics and other crises. Specifically, we want fundamental care delivery to be seen as the minimum standard rather than the exception across our health and care systems. We want nursing leaders to call out and stand up for the importance of building fundamental care into systems, processes and funding priorities. We do not want to see the voices of nursing leaders quashed or minimized in favour of other agendas. In turn, what we want to see is greater recognition of fundamental care work and greater respect for the people who do it. We expect nurses to have a ‘seat at the table’ where the key health and care decisions that impact patients and staff are made.ConclusionTo achieve our goals we must (1) ensure that fundamental care is embedded in all health and care systems, at all levels; (2) build on and strengthen the leadership skills of the nursing workforce by clearly advocating for person‐centred fundamental care; (3) co‐design systems that care for and support our staff's well‐being and which foster collective resilience rather than overly rely on individual resilience; (4) improve the science and methodologies around reporting and measuring fundamental care to show the positive impact of this care delivery and (5) leverage the COVID pandemic crisis as an opportunity for transformational change in fundamental care delivery.
Decision tree for identifying pertinent integration procedures and joint displays in mixed methods research
Authors: Younas Ahtisham , Durante Angela 
Published: December 2022

AbstractAimsTo propose a decision tree for identifying appropriate integration procedures and joint displays for achieving integration in mixed methods studies.DesignA methodological discussion.Data SourcesMethodological literature including mixed methods textbooks, methodological reviews and studies published in the last 10 years (2012–2022).Implications for NursingMixed methods are instrumental to study complex nursing care processes and health‐human phenomena. Nurse researchers can use this decision tree to choose the most appropriate integration procedures to overcome the integration challenge when designing and conducting mixed methods nursing studies.ConclusionIntegration procedures and joint displays are the most widely used methods for tackling the integration challenge in mixed methods research (MMR). The multifaceted and contingent nature of these methods are beneficial for their tailored and adapted use at the data collection, analysis, interpretation and reporting levels. The use of the most pertinent integration procedures and joint displays is critical for ensuring quality in MMR.ImpactA growing methodological literature on MMR offers a wide range of integration procedures and techniques. Therefore, choosing appropriate integration procedures and analysis methods can be challenging for nurse researchers interested in conducting mixed methods studies. A decision tree is developed outlining 14 integration procedures and their corresponding mixed methods designs, purposes and joint displays. Examples of mixed methods studies in the discipline of nursing are presented to illustrate the implementation of the integration procedures. The decision tree can serve as a straightforward methodological tool for decision making in MMR. Nurse researchers can effectively use this decision tree for research and teaching purposes.Patient or Public ContributionNo direct patient or public contribution.
Coding energy knowledge in constructed responses with explainableNLPmodels
Authors: Gombert Sebastian , Di Mitri Daniele , Karademir Onur , Kubsch Marcus , Kolbe Hannah , Tautz Simon , Grimm Adrian , Bohm Isabell , Neumann Knut , Drachsler Hendrik 
Published: December 2022

AbstractBackgroundFormative assessments are needed to enable monitoring how student knowledge develops throughout a unit. Constructed response items which require learners to formulate their own free‐text responses are well suited for testing their active knowledge. However, assessing such constructed responses in an automated fashion is a complex task and requires the application of natural language processing methodology. In this article, we implement and evaluate multiple machine learning models for coding energy knowledge in free‐text responses of German K‐12 students to items in formative science assessments which were conducted during synchronous online learning sessions.DatasetThe dataset we collected for this purpose consists of German constructed responses from 38 different items dealing with aspects of energy such as manifestation and transformation. The units and items were implemented with the help of project‐based pedagogy and evidence‐centered design, and the responses were coded for seven core ideas concerning the manifestation and transformation of energy. The data was collected from students in seventh, eighth and ninth grade.MethodologyWe train various transformer‐ and feature‐based models and compare their ability to recognize the respective ideas in students' writing. Moreover, as domain knowledge and its development can be formally modeled through knowledge networks, we evaluate how well the detection of the ideas within responses translated into accurate co‐occurrence‐based knowledge networks. Finally, in terms of the descriptive accuracy of our models, we inspect what features played a role for which prediction outcome and if the models pick up on undesired shortcuts. In addition to this, we analyze how much the models match human coders in what evidence within responses they consider important for their coding decisions.ResultsA model based on a modified GBERT‐large can achieve the overall most promising results, although descriptive accuracy varies much more than predictive accuracy for the different ideas assessed. For reasons of comparability, we also evaluate the same machine learning architecture using the SciEntsBank 3‐Way benchmark with an English RoBERTa‐large model, where it achieves state‐of‐the‐art results in two out of three evaluation categories.