bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resveratrol reverses TGF‐β1‐mediated invasion and metastasis of breast cancer cells via the SIRT3/AMPK/autophagy signal axis
Authors: Wang Jia , Huang Ping , Pan Xiafang , Xia Chunhua , Zhang Hong , Zhao Han , Yuan Zhao , Liu Jianming , Meng Chao , Liu Fanglan 
Published: September 2022

AbstractResveratrol (Resv) has antitumorigenic and antimetastatic activities; however, the molecular mechanisms underlying the inhibitory effects of Resv on the invasion and metastasis of breast cancer cells are still a subject of debate. In our study, we demonstrated that Resv inhibited tumor cell proliferation and tumor growth. It also suppressed invasion and pulmonary metastasis of breast cancer by reversing the transforming growth factor beta 1 (TGF‐β1)‐mediated EMT process. Meanwhile, the anticarcinogenic effects of Resv were abolished by the autophagy blocker 3‐methyladenine (3‐MA) or Beclin 1 small interfering RNA. Moreover, Resv upregulated autophagy‐related genes and protein levels and induced the formation of autophagosomes in 4T1 breast cancer cells and xenograft mice, suggesting that autophagy was involved in the anticarcinogenic activities of Resv in both models. In addition, Resv‐induced autophagy by increasing the expression of SIRT3 and phosphorylated AMPK. SIRT3 knockdown reduced AMPK phosphorylation and autophagy‐related proteins levels, and suppressed the anticancer effects of Resv, demonstrating that the inhibitory effects of Resv on tumor progression were mediated via the SIRT3/AMPK/autophagy pathway. Taken together, our study provided novel insight into the anticancer effects of Resv and revealed that targeting the SIRT3/AMPK/autophagy pathway can serve as a new therapeutic target against breast cancer.
Lutein ameliorates high‐fat diet‐induced obesity, fatty liver, and glucose intolerance in C57BL/6J mice
Authors: Gopal Sowmya Shree , Sukhdeo Shinde Vijay , Vallikannan Baskaran , Ponesakki Ganesan 
Published: September 2022

AbstractObesity is a multi‐factorial metabolic syndrome that increases the risk of cardiovascular diseases, diabetes, and cancer. We recently demonstrated the antiadipogenic efficacy of lutein using a 3 T3‐L1 cell culture model. This study aimed to examine the antiobesity efficacy of lutein on high‐fat (60% kcal fat) diet‐induced C57BL/6J obese mice model. Lutein (300 and 500 μM), Orlistat (30 mg/kg body weight ‐ positive control), and its combination (orlistat, 15 mg/kg body weight+lutein, 300 μM) were administered in high‐fat diet (HFD)‐fed mice every other day for 24 weeks. The effect on serum and hepatic lipid parameters was estimated using biochemical assay kits. The adipose tissue expression of adipocyte differentiation markers at gene and protein levels was analyzed by RT‐PCR and western blotting, respectively. The results showed that lutein administration and drug significantly reduced epididymal and abdominal adipose tissue weights. Further, lutein reduced the serum cholesterol and LDL‐C concentration compared to the HFD group. The HFD‐induced elevation in the hepatic triglycerides and cholesterol levels were significantly blocked by lutein and its combination with the drug. Similarly, lutein and its drug combination efficiently lowered the HFD‐mediated elevated blood glucose levels. Lutein downregulated the expression of CEBP‐α, PPAR‐γ, and FAS in the epididymal adipose tissue. Thus, supplementation of lutein may control diet‐induced obesity and associated complications in the human population.
Explore the key factors of sustainable development: A bibliometric and visual analysis of technological progress
Authors: Ren Wenhan , Ni Jing , Jiao Wen , Li Yan 
Published: September 2022

AbstractTechnological progress is an important way to realize the coordinated and stable development of economy and environment, and it is also a key factor to promote sustainable development. In the face of a large amount of literature data, traditional qualitative literature analysis methods have certain limitations and cannot objectively show the frontier trends of the research. Therefore, this article uses knowledge graph quantification and bibliometric analysis as research methods to quantitatively analyze technological progress. With the help of CiteSpace bibliometric tool and Web of Science database, this article draws the knowledge map, showing the basic characteristics, evolution logic, and hot frontiers of technological progress in the past 30 years, in order to grasp the new trend of technological progress research. The results show that: the number of studies on technological progress is increasing year by year, and China is the country with the largest number of publications. Research on technological progress mainly focuses on resource labor‐intensive industries on land, while less attention on ocean. The evolution of technological progress is characterized by three stages: theoretical exploration (1997–2005), empirical analysis (2006–2016), and application exploration (2017–2021), and the path analysis and the impact mechanism of technological progress in the field of energy conservation and emission reduction are the focus of scholars at this stage. Finally, this article makes prospects from the aspects of research perspective, theme and content, so as to provide a reference basis for promoting scientific decision‐making of sustainable development.
Biological Activity and Optical Sensor Properties of Green Synthesis Polymer
Authors: Bahçeci Dilek Şenol , Demir Neslihan , Kocaeren Aysel Aydın 
Published: September 2022

AbstractIn this study, 4‐amino‐3‐hydroxy‐1‐naphthalene sulfonic acid (Monomer) was converted to its enzyme‐catalyzed oxidative polymerization (Poly‐enz) in dioxane solvent by horseradish peroxidase enzyme (HRP) in the presence of hydrogen peroxide as oxidant. For the analysis of the monomer and its synthesized Poly‐enz, 1H‐NMR, 13C‐NMR, FT‐IR, TGA, and SEM measurements were determined. In addition, the optical ion sensor properties of Poly‐enz against metals such as Ag+, Cd2+, Co2+, Hg2+, Ni2+, Pb2+, Zn2+, Cu2+ were examined with UV‐Vis spectrophotometer. It was observed that Cu metal showed sensory properties. DNA cleavage and DNA binding activities of monomer and Poly‐enz were done using by agarose gel electrophoresis and UV‐Vis methods, respectively. It was seen that the Poly‐enz interacted with DNA electrostatically. This enzymatic polymer was found to cleave pBR322 plasmid DNA both hydrolytically and oxidatively as well. The antioxidant activities of the compounds were examined by three different methods (DPPH, ABTS, FRAP) and it was found that the Poly‐enz showed high antioxidant activity compared to its monomer. Antimicrobial activities were studied against various bacterial and yeast cultures by the minimum inhibition concentration (MIC) method. It was observed that the antimicrobial activities of the enzymatic polymer in different microorganisms increased depending on the concentration.
Investigation on the crack formation in carbon concrete for the use in deck caps on cantilever slabs of bridges
Authors: Farwig Kristina , Schulte‐Schrepping Christoph , Curbach Manfred , Breitenbücher Rolf 
Published: September 2022

AbstractDeck caps of bridges—in Germany shortly known as “bridge caps”—are usually made of steel reinforced concrete to form the anchoring area for the guardrail of bridge deck edges on existing cantilever slabs. Combined with protective devices, the outside arranged deck caps provide safety against lateral breaking or crashes of vehicles. Due to their exposed position in the cross section of bridges and the particularly intensive stresses, the deck caps are considered as wear parts and have to be renewed several times during the service life of a bridge. The decisive factor here is the discrepancy between the frost resistance of the concrete and the crack width restriction. On the one hand, only very small cracks (<0.2 mm) can be accepted to prevent corrosion of the rebars under the strong exposure of these elements. To confine crack widths without applying an excessively high degree of reinforcement, a low concrete compressive strength is advantageous. On the other hand, a sufficient frost resistance requires a correspondingly high compressive strength. With carbon reinforcement, these contrary points could be defused and simultaneously the needed durability could be provided. Therefore, slightly modified deck cap concretes combined with a carbon reinforcement mesh were tested to examine the bond behavior with and without freeze–thaw attack. To prove the characteristics of this combined system, the crack formation and crack distribution were investigated experimentally. The test results were compared to calculated values from a mathematically tool to be able to develop different reinforcement concepts in future that can ensure an optimized crack formation and crack width for deck caps.
ASRpro: A machine‐learning computational model for identifying proteins associated with multiple abiotic stress in plants
Authors: Meher Prabina Kumar , Sahu Tanmaya Kumar , Gupta Ajit , Kumar Anuj , Rustgi Sachin 
Published: September 2022

AbstractOne of the thrust areas of research in plant breeding is to develop crop cultivars with enhanced tolerance to abiotic stresses. Thus, identifying abiotic stress‐responsive genes (SRGs) and proteins is important for plant breeding research. However, identifying such genes via established genetic approaches is laborious and resource intensive. Although transcriptome profiling has remained a reliable method of SRG identification, it is species specific. Additionally, identifying multistress responsive genes using gene expression studies is cumbersome. Thus, endorsing the need to develop a computational method for identifying the genes associated with different abiotic stresses. In this work, we aimed to develop a computational model for identifying genes responsive to six abiotic stresses: cold, drought, heat, light, oxidative, and salt. The predictions were performed using support vector machine (SVM), random forest, adaptive boosting (ADB), and extreme gradient boosting (XGB), where the autocross covariance (ACC) andK‐mer compositional features were used as input. With ACC,K‐mer, and ACC +K‐mer compositional features, the overall accuracy of ∼60–77, ∼75–86, and ∼61–78% were respectively obtained using the SVM algorithm with fivefold cross‐validation. The SVM also achieved higher accuracy than the other three algorithms. The proposed model was also assessed with an independent dataset and obtained an accuracy consistent with cross‐validation. The proposed model is the first of its kind and is expected to serve the requirement of experimental biologists; however, the prediction accuracy was modest. Given its importance for the research community, the online prediction application, ASRpro, is made freely available (https://iasri‐sg.icar.gov.in/asrpro/) for predicting abiotic SRGs and proteins.
Gallbladder polyps growth rate is an independent risk factor for neoplastic polyps
Authors: Han Ji Won , Choi Young Hoon , Lee In Seok , Choi Ho Joong , Hong Tae Ho , You Young Kyoung 
Published: September 2022

AbstractBackgroundThe size of gallbladder (GB) polyps is a representative risk factor for neoplastic polyps. However, whether growth rate during follow‐up is associated with neoplastic polyps remains unclear.MethodsFrom 2009 to 2019, a cohort of patients with GB polyps who underwent cholecystectomy was enrolled. We included only patients who underwent at least two abdominal ultrasonography procedures at least 6 months apart prior to cholecystectomy. Performance and optimal cutoff value of polyp growth rate for predicting neoplastic polyps were estimated using receiver operating characteristic (ROC) analysis. In addition to growth rate, several other variables considered suitable for predicting neoplastic polyps were also investigated. A nomogram was created to predict neoplastic polyps.ResultsA total of 239 patients with neoplastic polyps (n = 27, 11.3%) and non‐neoplastic polyps (n = 212, 88.7%) were included. The median follow‐up period was 28.5 months. The area under the ROC curve (AUROC) of polyp growth rate for neoplastic polyps was 0.66 (95% confidence interval, 0.59–0.72). The growth rate cutoff value for prediction of neoplastic polyps was 3 mm/year (sensitivity, 37.0%; specificity, 86.3%). Multivariate analysis identified several factors predicting neoplastic polyps: polyp size ≥10 mm (odds ratio [OR], 3.74, p = 0.041), solitary polyp (OR, 3.92, p = 0.004), and polyp growth rate ≥ 3 mm/year (OR, 2.75, p = 0.031). The AUROC of the nomogram using these three significant factors in multivariate analysis was 0.71.ConclusionGB polyps with a growth rate of over 3 mm per year on ultrasonography during follow‐up should be considered a risk factor for neoplastic polyps.
Fetal left‐atrial posterior‐space‐to‐diagonal ratio at 17–37 weeks' gestation for prediction of total anomalous pulmonary venous connection
Authors: Anuwutnavin S. , Unalome V. , Rekhawasin T. , Tongprasert F. , Thongkloung P. 
Published: September 2022

ABSTRACTObjectivesTo develop normative data for the left‐atrial posterior‐space‐to‐diagonal (LAPSD) ratio and post‐left atrium space (PLAS) index in fetuses from 17 to 37 weeks' gestation, and determine the optimal cut‐offs of the LAPSD ratio and PLAS index to discriminate between normal fetuses and those with total anomalous pulmonary venous connection (TAPVC).MethodsThis was a prospective cross‐sectional assessment of 428 structurally normal fetuses between 17 and 37 weeks' gestation and a retrospective study of 27 TAPVC fetuses. The fetal LAPSD ratio was calculated by dividing the left atrium–descending aorta distance (LDD) by the left atrial diagonal diameter (LA). The PLAS index was calculated as the ratio of the LDD to the descending aorta diameter (DA). Pearson's correlation analysis was used to examine the correlation of cardiac parameters with gestational age (GA) and fetal somatic growth. The PLAS index and LAPSD ratio were compared between the normal and TAPVC groups to assess their usefulness in the prenatal diagnosis of TAPVC.ResultsLDD, LA and DA measures showed moderate to strong positive correlation, whereas both the LAPSD ratio and PLAS index showed a slight decrease with increasing GA and biometric variables. The fetal LAPSD ratio and PLAS index in TAPVC cases were significantly greater compared with those of fetuses with a normal heart (both P < 0.001). There were no significant differences in the PLAS index and LAPSD ratio between the isolated and complex TAPVC groups (both P = 1). No significant associations of the PLAS index and LAPSD ratio with fetal gender, four‐chamber view type (apical, basal or lateral) or TAPVC type were found. Using values of ≥ 0.35 for the LAPSD ratio and of ≥ 1 for the PLAS index exhibited similar excellent diagnostic performance, with a sensitivity of 100% and specificity of 97.0% or 95.1% for detecting TAPVC.ConclusionsOur study demonstrates that the LAPSD ratio is a practical and effective screening tool for diagnosing fetal TAPVC, similar to the PLAS index. Incorporating these parameters into routine cardiac scanning may enhance the prenatal detection of TAPVC. © 2022 International Society of Ultrasound in Obstetrics and Gynecology.
Feasibility and efficacy of an online compassion‐focused imagery intervention for veterinarian self‐reassurance, self‐criticism and perfectionism
Authors: Wakelin Katherine E. , Perman Gemma , Simonds Laura M. 
Published: September 2022

AbstractBackgroundVeterinarians report high levels of psychological distress and self‐criticism. However, there is minimal research investigating psychological interventions for veterinarians. Evidence suggests that compassion‐focused therapy is effective at reducing distress in those with high self‐criticism. This study aimed to investigate the feasibility and preliminary effectiveness of a 2‐week online compassionate imagery intervention for veterinarians.MethodsA one‐group repeated measures design was used with 128 veterinarians. Participants completed measures of perfectionism, self‐criticism, self‐reassurance and fears of compassion four times, at 2‐week intervals (at baseline, pre‐intervention, post‐intervention and 2‐week follow‐up). Participants answered written questions about their intervention experience post‐intervention.ResultsContent analysis of the qualitative data found the intervention to be acceptable and beneficial to participants. Overall, study attrition was 50.8%, which is reasonable for a low‐cost intervention. Minimal differences were found between participants who dropped out compared to those who completed the intervention. Perfectionism, work‐related rumination and self‐criticism were significantly reduced post‐intervention, and these effects were maintained at follow‐up. Resilience and self‐reassurance remained unchanged. Fears of compassion reduced over the baseline period and pre–post intervention, questioning the validity of the measure.ConclusionOverall, in the context COVID‐19, the intervention showed impressive feasibility and preliminary effectiveness. Randomised control trials are recommended as the next step for research to establish the intervention's effectiveness.
Neurolymphomatosis in a cat with diffuse neuromuscular signs including cranial nerve involvement
Authors: van Koulil Quinten , Santifort Koen M. , Beukers Martijn , Ioannidis Marianthi , Van Soens Iris 
Published: September 2022

AbstractThis case report describes the clinical signs, electrodiagnostic findings, magnetic resonance imaging, cerebrospinal fluid results and histopathology in a cat with neurolymphomatosis. A 7‐year‐old domestic shorthair cat was presented for evaluation of subacute progressive signs of tetraparesis, ataxia and anorexia. Neurological examination was consistent with a diffuse neuromuscular localisation with cranial nerve involvement. Electrodiagnostic test results were suggestive of denervation due to axonopathy. Magnetic resonance imaging of the brain and cervical spinal cord showed severe enlargement and contrast‐enhancement of both trigeminal nerves, brachial plexi and the right C2 and C3 spinal nerves. Cerebrospinal fluid cytology was consistent with lymphoma. Histopathology confirmed infiltration of malignant lymphoid cells in both trigeminal nerves, the spinal nerve roots of C6–T2, meninges and choroid plexi. Immunohistochemistry results were consistent with a diagnosis of T‐cell lymphoma. Although not frequently observed, lymphoma is a differential diagnosis in cats with diffuse neuromuscular signs and/or cranial nerve deficits.
River otter chemical immobilization and field surgery using nonscheduled drugs
Authors: Gettelman Tatiana E. , Nielsen Clayton K. , Scimeca Joseph M. , Schauber Eric M. 
Published: September 2022

AbstractRadiotelemetry provides valuable information about wildlife populations that is often unattainable using other methods. Many vertebrate species can carry radiotransmitters on collars or harnesses, but radiotelemetry can be more problematic for other species. River otters (Lontra canadensis) are typically radiomarked by surgically implanting intra‐abdominal transmitters under general anesthesia. However, surgical requirements and restrictions on some immobilizing drugs may limit options for implanting river otters with radiotransmitters in the field. To address these issues, we developed a protocol for chemically immobilizing live‐captured river otters with nonscheduled drugs (by estimated weight class; dexmedetomidine 0.06 mg/kg and nalbuphine 1.2 mg/kg) and conducting field surgeries at capture sites. We surgically implanted 23 river otters with radiotransmitters in southern Illinois during November 2018−March 2020. A plane of anesthesia suitable for surgery was maintained with isoflurane (1−4%). After surgery, capture drugs were reversed with atipamezole and river otters were typically released in 30−60 min. We recorded one surgery‐related mortality and one post‐surgery mortality. Our surgical method for radiomarking river otters minimized stress and handling time without compromising their health and demonstrated the efficacy of less‐restricted chemical immobilization agents for river otters.
Understanding Risks and Moral Emotions in the Context of COVID-19 Policy Making: The Case of the Netherlands
Authors: Roeser Sabine 
Published: September 2022

AbstractIn most countries, including the Netherlands, decision-making about the COVID-19 policy measures was initially based on medical information, and only later did it also include insights from social sciences. However, ethical implications of COVID-19 policy measures have not frequently been explicitly considered. As a result, critical ethical issues have been overlooked, and values, concerns, and emotions have not been considered appropriately. In this chapter, I will argue that emotions can help to make important moral dilemmas around decision-making about COVID-19 explicit and to make ethically justified decisions. I will do so by zooming in specifically on how the Netherlands has handled the pandemic so far. My discussion aims to contribute to morally better and more socially acceptable decision-making about the challenges that COVID-19 poses, as well as to hopefully learn lessons for possible future pandemics.
Values as Hypotheses and Messy Institutions: What Ethicists Can Learn from the COVID-19 Crisis
Authors: Pesch Udo 
Published: September 2022

AbstractIn this chapter, the COVID-19 crisis is examined as an episode that reveals various complications in the relation between values and institutions. I argue that these complications cannot be addressed satisfactorily by ethics, as this field is characterised by a gap between the identification of values worth pursuing and the effectuation of these values in society through politics. This chapter aims to bridge this gap between ethics and politics by outlining the dialectical relation between values and institutions. It will do so by first presenting values as collectively held understandings that emerge in public deliberation. Second, these values are safeguarded by setting up appropriate institutions, which, at the same time, also allows the further substantiation of these values. However, it also needs to be acknowledged that institutions are not mere instrumental solutions to further societal values. On the contrary, they have their own morally laden dynamics. As such, they should also be susceptible to adjustment following societal demand.
Designing in Times of Uncertainty: What Virtue Ethics Can Bring to Engineering Ethics in the Twenty-First Century
Authors: Bergen Jan Peter , Robaey Zoë 
Published: September 2022

AbstractOur world is changing in rapid and unanticipated ways. Given technology’s central role in those changes, engineers face difficult design decisions. In dominant consequentialist and deontological engineering ethics paradigms, making design choices implies having sufficient information on those choices and their trade-offs, which is often lacking. Some scholars have pointed to virtue ethics as an alternative approach to engineering ethics, but how can virtue ethics support engineers in situations of uncertainty? In this chapter, we explore how virtue ethics is conducive to sound engineering in different conditions of uncertainty.