bluuebunny/crossref_metadata_2025_split huggingface dataset:
A c-Fos activation map in NTG/levcromakalim-induced chronic migraine mice
Authors: Wu Shouyi , Ren Xiao , Zhu Chenlu , Liu Xuejiao , Zhang Kaibo , Li Zhi lei , Wang Yonggang 
Published: September 2022

Abstract
Background
Chronic migraine is a common and disabling disorder. Functional MRI has established that abnormal brain region activation is present in chronic migraine. Drugs targeting the calcitonin gene-related peptide (CGRP) or its receptor have been reported to be efficient for treating chronic migraine. The CGRP signaling pathway has been documented in two types of preclinical migraine mouse models. However, it remains unclear how an active specific brain region develops migraine-like pain and whether CGRP receptor antagonists can alter specific brain region activation and relieve migraine-like pain. Therefore, we sought to investigate brain activation and the effect of olcegepant treatment on brain activation in two chronic migraine models and provide a reference for future research on neural circuits.
Methods
Repeated administration of nitroglycerin (NTG) or levcromakalim(LEV) was conducted to establish two types of preclinical migraine mouse models to stimulate human migraine-like pain. Mechanical hypersensitivity was evaluated using the von Frey filament test. Then, we evaluated the activation of different brain regions using c-Fos and NeuN staining. Olcegepant, a CGRP receptor-specific antagonist, was administered to explore its countering effect on brain region activation and mechanical hyperalgesia.
Results
After treatment with NTG and LEV, acute and chronic basal mechanical hyperalgesia was observed in the migraine models. Olcegepant, a CGRP receptor selective antagonist, significantly alleviated mechanical hyperalgesia in both models. In NTG-induced chronic migraine mice, the medial prefrontal cortex (mPFC), anterior cingulate cortex (ACC), and caudal part of the spinal trigeminal nucleus (Sp5c) showed a significant increase in c-Fos expression, while olcegepant reduced c-Fos expression. No change in c-Fos expression was found in the paraventricular thalamic nucleus (PVT) and ventrolateral periaqueductal gray (vlPAG). In LEV-induced migraine mice, mPFC, PVT and Sp5c showed a significant increase in c-Fos expression and olcegepant reduced c-Fos expression. No change in c-Fos expression was found in vlPAG and ACC.
Conclusions
Our study demonstrated activation of the medial prefrontal cortex and caudal part of the spinal trigeminal nucleus in both chronic migraine models. Olcegepant may alleviate hyperalgesia of the hind paw and periorbital area by attenuating brain activation in chronic migraine.
Optimization of sunlight photo-catalytic treatment coupled to a coagulation-flocculation process
Authors: Raissi Sahar , fakhfakh fatma , Hadrich bilel 
Published: September 2022

Abstract
The oleic industry is one of the most polluting in food industry sector. Among other harmful by-products, it generates charged sludge. Coagulation–flocculation is a relatively simple physico–chemical technique that ensures excellent separation of solid particles from dissolved ones. To obtain acceptable treated water quality, this method is used along with TiO2-based sunlight photo-catalysis. We optimize this binary process to treat mill wastewater collected from the region of Mahdia, east center of Tunisia, by applying a combination of mixture design (3 components-mixture: lime water, iron solution and cactus mash) and full factorial design 22 (2 process factors: dilution ratio (aqueous solution/water) and used TiO2 mass). Both the phenolic compounds removal and solution decolorization are closely monitored. The quadratic models for those two responses indicate optimum conditions to a coagulation-flocculation performance using a mixture of 48.48% of lime water with 51.52% of cactus mash with low level of dilution rate and high level of TiO2 mass. Experience of those conditions validated calculi, and they give a decolorization of 92.57 ± 0.90% and an organic degradation of 96.19 ± 0.97% in good concordance with the mathematic predictions that yield 93.06% of decolorization and 98.70% of organic degradation.
Association between uric acid and depressive symptoms in older adults: The Korea National Health and Nutrition Examination Survey
Authors: Kim Joo O , Park Gyu Nam , Oh Jae Won , Lee San 
Published: September 2022

Abstract

Uric acid is known for its antioxidant capacity; however, whether uric acid is independently associated with depression in the older population remains controversial. The current study aimed to investigate the association between uric acid and depressive symptoms in older adults according to sex, using a large national sample population. Data from the 2016, 2018, and 2020 Korean National Health and Nutrition Examination Surveys were analyzed, and 5609 participants older than 60 years were included in this study. We defined a Patient Health Questionnaire-9 score ≥ 5 as indicating the presence of depressive symptoms. The prevalence of depressive symptoms was higher in women with lower uric acid levels than in those with higher uric acid levels. In women, uric acid was significantly associated with depressive symptoms in the multivariable logistic regression analysis (odds ratio, 1.36; 95% confidence interval, 1.10–1.68; p = 0.005). However, no significant association between uric acid levels and depressive symptoms was observed in men. The findings of this study suggest that uric acid is associated with depressive symptoms in older women, but not in men. Further research is needed on sex differences in the relationship between serum uric acid levels and depressive symptoms.
Cadmium toxicity promotes hormonal imbalance and induces systemic resistances in barley
Authors: Neyshabouri Fatemeh Alzahra , Ghotbi-Ravandi Ali Akbar , Shariatmadari Zeinab , Tohidfar Masoud 
Published: September 2022

Abstract
Cadmium (Cd) is a widely distributed pollutant that adversely affects plants’ metabolism and productivity. Phytohormones play a vital role in the acclimation of plants to metal stress. On the other hand, phytohormones trigger systemic resistances, including systemic acquired resistance (SAR) and induced systemic resistance (ISR), in plants in response to biotic interactions. The present study aimed to investigate the possible induction of SAR and ISR pathways in relation to the hormonal alteration of barley seedlings in response to Cd stress. Barley seedlings were exposed to 1.5 mg. g− 1 Cd in the soil for three days. Cd accumulation resulted in a reduction in the nutrient content of barley seedlings. The specific activity of superoxide dismutase and the content of hydrogen peroxide significantly increased in response to Cd toxicity. The content of abscisic acid, jasmonic acid, and ethylene increased under Cd exposure. Cd treatment resulted in the upregulation of NPR1, PR3, and PR13 genes in SAR pathways. The transcripts of PAL1 and LOX2.2 genes in the ISR pathway were also significantly increased in response to Cd treatment. These findings suggest that hormonal-activated systemic resistances are involved in the tolerance of barley to Cd stress.
Microstructural, mechanical, and electrochemical analysis of carbon doped AISI carbon steels
Authors: Ishtiaq Muhammad , Inam Aqil , Tiwari Saurabh , Seol Jae Bok 
Published: September 2022

Abstract
The effect of carbon doping contents on the microstructure, hardness, and corrosion properties of heat-treated AISI steel grades of plain carbon steel was investigated in this study. Various microstructures including coarse ferrite-pearlite, fine ferrite-pearlite, martensite, and bainite were developed by different heat treatments i.e. annealing, normalizing, quenching, and austempering, respectively. The developed microstructures, micro-hardness, and corrosion properties were investigated by a light optical microscope, scanning electron microscope, electromechanical (Vickers Hardness tester), and electrochemical (Gamry Potentiostat) equipment, respectively. The highest corrosion rates were observed in bainitic microstructures (2.68 – 12.12 mpy), whereas the lowest were found in the fine ferritic-pearlitic microstructures (1.57- 6.36 mpy). A direct correlation has been observed between carbon concentration and corrosion rate, i.e. carbon content resulted in an increase in corrosion rate (2.37 mpy for AISI 1020 to 9.67 mpy for AISI 1050 in annealed condition).
Identifying the functional pathways and potential genes associated with interferon signaling during human adenovirus type 7 by weighted gene co-expression network analysis
Authors: Yang Zhongying , Wei Jianhua , He Yu , Ren Luo , Chen Shiyi , Deng Yu , Zang Na , Liu Enmei 
Published: September 2022

Abstract
Human adenovirus type 7 (HAdV-7) can cause severe pneumonia and complications in children. However, the pathogenesis and genes involved remain largely unknown. We collected HAdV-7 infected and mock-infected A549 cells at 24, 48, and 72 hours post-infection (hpi) for RNA sequencing (RNA-seq), and identified potential genes and functional pathways associated with HAdV-7 infection using weighted gene co-expression network analysis (WGCNA).Based on bioinformatics analysis, 12 co-expression modules were constructed by WGCNA, with the blue, tan, and brown modules significantly positively correlated with Ad-24 hpi, Ad-48 hpi, and Ad-72 hpi, respectively. Functional enrichment analysis indicated that the blue module was mainly enriched in DNA replication and viral process; the tan module was largely enriched in metabolic pathways and regulation of superoxide radical removal; and the brown module was predominantly enriched regulation of cell death. qPCR was used to determine transcript abundance of some identified hub genes, and results were consistent with those from RNA-seq. Comprehensively analyzing hub genes and differentially expressed genes in the GSE68004 dataset, we identified SOCS3, OASL, ISG15, and IFIT1 as potential candidate genes for biomarkers or drug targeting in HAdV-7 infection. Further, we propose a multi-target inhibition of interferon signaling mechanism to explain the HAdV-7 associated with higher severity of clinical consequences. Overall, we constructed a framework of co-expression gene modules from A549 cells infected with HAdV-7, thus providing a basis for identifying potential genes and pathways involved in adenovirus infection and for studying the pathogenesis of adenoviruses.
Influence of Adsorption of Gold and Silver Nanoclusters on Structural, Electronic, and Nonlinear optical properties of Pentacene-5,12-dione: A DFT study
Authors: Lakhera Shradha , Rana Meenakshi , Devlal Kamal 
Published: September 2022

Abstract
The present study deals with the investigation of the change in the hyperpolarizability of Pentacene-2,5-dione (PD) on the introduction of the gold (Au) and silver (Ag) nanoclusters using the density functional theory (DFT). The involvement of the nanoclusters induces the intramolecular interactions, which is confirmed by the electrostatic potential plot and Mulliken charge distribution. The process of adsorption of metal clusters on the surface of PD is investigated by absorption spectra and the Raman spectra analysis. The global reactivity parameters validate a hike in the reactivity of the PD after the adsorption of Ag3 and Au3 nanoclusters. The study reported extraordinary enhancement in the hyperpolarizability of the metal nanoclusters adsorbed complexes. The high value of hyperpolarizability for PD-Ag3 (184.22×10− 30esu) and PD-Au3 (2057.26×10− 30esu) validates their applications in highly efficient NLO devices. The statistical calculation of radiative lifetime and light-harvesting efficiency reveals that probe PD can fruitfully contribute to the development of photo luminescent material and photosensitizer dye in dye synthesized solar cells.
Understanding the perception and requirements of a plant-based nutrition app for cancer patients
Authors: Dalayya Shekinah , Elsaid Salma Tamer Fathy Ahmed , Ng Kher Hui , Song Tien Lii , Lim Joanne Bee Yin 
Published: September 2022

Abstract
Understanding human perception and requirements on nutrition for cancer prevention and condition management is important so that nutrition applications can be catered for cancer patients. In this paper, web-scraping was conducted to understand the public’s perception, attitude and requirements related to a plant-based diet as a recommended diet for cancer prevention and condition management. Text and sentiment analysis were carried out on results gathered from 73 social sites to determine whether non-cancer and cancer patients use plant-based diets, how they have been consumed, their benefits in the prevention and condition management of cancers, the existing myths/fake news about cancer and what do cancer patients need in a nutrition app. Results of the text analysis highlight missing gaps in existing apps to include a lack of credibility and endorsement by professionals. Future nutrition apps should provide personalized diet, symptoms management, good user experience, credibility, and emotional and mental health support.
Abandonment of salvage logging in windthrown stands is key to protection of late-succession carabid assemblages
Authors: Skłodowski Jarosław 
Published: September 2022

Abstract
The usual practice in forest stands following a windthrow event is to perform salvage logging of all broken trees with their crowns before seedlings are planted. Does leaving broken tree crowns on the ground afford shelter for forest species? Epigeic carabids (Coleoptera) were the test taxon. The following hypotheses were put forward: (1) the more severe the disturbance, the higher the abundance and species richness, and the lower the proportion of late successional fauna in carabid assemblages, (2) crowns of pine trees lying on the ground support a higher proportion of late-successional carabid species, which effect should be more pronounced in more severely disturbed stands, but will decrease as needles fall off over the next 3 years. In order to verify the first hypothesis, 3 variants of stand disturbance (severely, moderately and least disturbed stands) were distinguished. The second hypothesis was verified by distinguishing additional variants at locations under and between tree crowns in the severely and moderately disturbed stands. Hypothesis 1 was proved correct and hypothesis 2 was proved partially correct. Simultaneously, it was demonstrated that the importance of the leaf area index for carabids decreased over the 3 years, while the significance of environmental indices, such as soil respiration rate and soil humidity, increased. This study indicates that there is a rationale for leaving in place at least some crowns lying on the ground in windthrown forest stands to provide shelter for forest species.
Augmented and Virtual Reality Usage in Awake Craniotomy: A Systematic Review
Authors: Mofatteh Mohammad , Mashayekhi Mohammad Sadegh , Arfaie Saman , Chen Yimin , Mirza Asfand Baig , Fares Jawad , Bandyopadhyay Soham , Henich Edy , Liao Xuxing , Bernstein Mark 
Published: September 2022

Abstract
Background Augmented and virtual reality (AR, VR) are becoming promising tools in neurosurgery. AR and VR can reduce challenges associated with conventional approaches via the simulation and mimicry of specific environments of choice for the surgeons. Awake craniotomy (AC) enables the resection of lesions from eloquent brain areas while monitoring higher cortical and subcortical functions. Both surgeons and patients may benefit from the various applications of AR and VR in AC. We aimed to investigate the application of AR and VR in AC and assess its prospective utility in neurosurgery.
Methods A systematic review of the literature was performed using PubMed, Scopus and Web of Science databases from inception to May 20th 2022 in accordance with the PRISMA guidelines.
Results Our search results yielded 220 articles. Six articles consisting of 118 patients were included in this review. VR was used in four papers, and the other two used AR. Tumour was the most common pathology in 108 patients, followed by vascular lesions in eight patients. VR was used for intraoperative mapping of language, vision, and social cognition, while AR was incorporated in preoperative training of white matter dissection and intraoperative visualisation and navigation. Overall, patients and surgeons were satisfied with the applications of AR and VR in their cases.
Conclusion AR and VR can be safely incorporated during AC to supplement and/or replace conventional approaches in neurosurgery. Future investigations are required to assess the feasibility of AR and VR in variousphases of AC.
Distinct Coordination patterns integrate Exploratory Head Movements with Whole-Body Movement Patterns during Walking
Authors: Andel Steven van , Schmidt Andreas , Federolf Peter 
Published: September 2022

Abstract
Visual guidance of gait is an important skill for everyday mobility and for prevention of falls in older adults. While this has often been studied using eye-tracking techniques, recent studies have shown that visual exploration involves more than just the eye; head movement and potentially the whole body is involved for successful visual exploration. Here, we use Principal Component Analysis (PCA) to assess to what extend whole-body movement patterns are related to exploratory head movement during gait. Twenty-one (after exclusions) healthy young adult volunteers followed a treadmill walking protocol designed to elicit different types of head movements (no stimuli compared to stimuli requiring horizontal, vertical, and mixed gaze shifts). PCA was used to establish whole-body correlated patterns of marker movement (Principal Movements; PMs) related to the activity of the head. In total 37 higher order PMs were found to be associated with head movement, two of these showed significant differences between trials associated with strong head rotations in the horizontal and sagittal plane. Both of these were associated with a whole-body pattern of activity. It was found that an analysis of the higher order components was required to establish that exploratory head movements are associated with distinct movement patterns across the body. This shows that visual exploration can produce movement patterns that are at direct contrast with the alleged aim of the postural system (to minimize body movement as much as possible) since they could have a destabilizing effect on the body. These findings shed new light on established results in visual search research and hold relevance for fall and injury prevention.
The Possible Security Issues of the DS-Lite IPv6 Transition Technology
Authors: Al-Azzawi Ameen , Lencse Gabor 
Published: September 2022

Abstract
This paper focuses on one of the most prominent IPv6 transition technologies named DS-Lite (Dual Stack Lite). The aim is to analyze the security threats that this technology might be vulnerable to. The analysis is based on the STRIDE method that stands for Spoofing, Tampering, Repudiation, Information disclosure and Elevation of privilege. Using the DFD (Data Flow Diagram) of a DS-Lite system, we summarized the potential security vulnerabilities and potential attack points within this infrastructure. We have also built a testbed for DS-Lite topology using several virtual machines, which were created using CentOS Linux images. We used our testbed to perform MitM (Man in the Middle) attack and other type of attacks against the B4 (border gateway) and the AFTR (Address Family Transition Router) and then to monitor their performance and the number of packets being translated under attack by different number of clients using several attacking toolkits.
Patients with skin cancer prefer to participate in procedure cost discussions: a cross-sectional survey
Authors: Bhandari Ritika , Rezac Laura , Pearlman Ross , Nahar Vinayak , Black William 
Published: September 2022

Abstract
For a given skin cancer, a number of treatment options are often available. The decision of which method to use is usually made by the treating physician. Despite significant changes to the healthcare system of the United States over the past ten years, healthcare costs continue to rise. These costs often affect patients in the form of higher deductibles, copays, and insurance premiums. The goal of this study was to determine patient attitudes regarding discussion of cost of skin cancer removal procedures and repairs. A 12-question survey was administered to 100 patients presenting for treatment of a skin cancer at an academic center. The first six questions addressed the importance the patient placed on treatment cost and related discussions, and the final six questions addressed repair cost. Greater than two-thirds of respondents felt that cost of both treatment (76%) and repair (67%) is somewhat or very important. Most patients reported that the cost of skin cancer treatment (56%) and repair (54%) should be considered by their surgeon. Furthermore, a majority of participants felt that cost differences should be discussed prior to treatment (67%) or repair (67%). Most respondents believed that cost discussion prior to treatment (64%) and repair (67%) would not affect their level of procedural anxiety. In conclusion, patients value cost discussions for treatment and repair of skin cancer. Surgeons should consider discussing these issues with patients in the appropriate clinical setting.
Potential health risk and level of heavy metal in groundwater in the highly-dense area of Akure, Southwestern Nigeria
Authors: OlajuwonOsisanya Wasiu , Akakuru Obinna Chigoziem , Eyankware Moses Oghenenyoreme , Ukor K. P. 
Published: September 2022

Abstract
The drinking water quality of Akure, in the Ondo State area of southwestern Nigeria, was assessed. 21 groundwater samples were collected and tested for physicochemical characteristics such as pH, main ions, and heavy metals from motorized boreholes and hand-dug wells. From the hydrogeochemical modeling, it shows 77.3% of the samples are of the geochemical zone of 4 (strong acids exceed weak acids) while 22.7% of the samples are of the geochemical zone of 3 (weak acids exceed strong acids) with a hydrogeochemical trend of Mg > Na + K > HCO3 > Cl > SO4 > Ca. WQI results revealed that groundwater needs proper treatment before it can be fit for different purposes. Contamination Factor results showed minimal contamination, Metal Pollution Index revealed that 80.6 percent of the samples had values more than 6, suggesting that they were severely contaminated. Pollution Load Index values in the area were less than 1 showing there isn't any pollution. Geoaccumulation Index results showed that 47.6% of the samples are respectable contamination while 47.6% of the samples are modestly dirty. Principal Component Analysis results indicated loadings of variables within the PCI, PC2, and PC3 while the Correlation Matrix results indicated that there exists weak correlation amongst elements and the majority of the elements. From statistical approach of health risk assessment, it was observed that traceable absorption of poisonous heavy metals have resulted to diarrhea, typhoid fever, cholera, hepatits A and dysentery commonly found in children and youths in the study area. The results of the Hazard index showed that children were more prone to heavy metal hazards. Regular monitoring of the groundwater sources is highly recommended.