bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tubulin engineering by semisynthesis reveals that polyglutamylation directs detyrosination
Authors: Ebberink Eduard , Fernandes Simon , Hatzopoulos Georgios , Agashe Ninad , Guidotti Nora , Reichart Timothy M. , Reymond Luc , Velluz Marie-Claire , Schneider Fabian , Pourroy Cédric , Janke Carsten , Gönczy Pierre , Fierz Beat , Aumeier Charlotte 
Published: September 2022

AbstractMicrotubules, a critical component of the cytoskeleton, carry combinations of post-translational modifications (PTMs), which are critical for the regulation of key cellular processes. Long-lived microtubules, in neurons particularly, exhibit both detyrosination of α-tubulin as well as polyglutamylation. Dysregulation of these PTMs results in disease, including developmental defects and neurodegeneration. Despite their importance, the mechanisms governing the emergence of such PTM patterns are not well understood, mostly because tools to dissect the function and regulation of tubulin PTMs have been lacking. Here, we report a chemical method to produce fully functional tubulin carrying precisely defined PTMs within its C-terminal tail. Using a sortase- and intein-mediated tandem transamidation strategy, we ligate synthetic α-tubulin tails, which are site-specifically glutamylated to specific extents, to recombinant human tubulin heterodimers. Using microtubules reconstituted with such designer tubulins, we show that polyglutamylation of α-tubulin promotes its detyrosination by enhancing the activity of the tubulin tyrosine carboxypeptidase vasohibin/SVBP in a manner dependent on the length of polyglutamyl chains. Moreover, modulating polyglutamylation levels in cells results in corresponding changes in detyrosination. Together, using synthetic chemistry to produce tubulins carrying defined PTMs, we can directly link the detyrosination cycle to polyglutamylation, connecting two key regulatory systems that control tubulin function.
Rethinking recruitment ethically through the lens of corporate social responsibility (CSR)
Authors: Benuyenah Vic 
Published: September 2022

PurposeTraditional recruitment practices tend to be rigorous, but some of its elements can be improved. The length of applications, missing or unstated salary details and lack of anonymity in some emerging countries' recruitment processes mean that recruitment standards remain questionable and unethical. This paper presents a conceptual discourse on how current recruitment activities can be improved in light of progress made with global Internet infrastructure and CSR standards.Design/methodology/approachThis is primarily a conceptual paper intended to discuss unethical recruitment practices. The author used selected studies and case studies to present the case for improvements in the field of recruitment.FindingsCompared with easy apply options popularised through recruitment websites, organisations continue to favour traditionally lengthy and complicated applications. The incidence of unstated salary, lack of anonymity and poor communication from some recruiters imply that more has to be done in these areas of CSR.Research limitations/implicationsAs with most conceptual papers, this study lacks adequate empirical support. The claims and propositions made are largely based on a scanty number of current research and industry observations.Practical implicationsThis study will have potential application in scenarios where recruiters are seeking to improve their practice; however, the recommendations may not be applicable to all organisations.Social implicationsThe understanding of ethical values and their application to recruitment will vary from culture to culture.Originality/valueThe paper offers a clear path to debating recruitment ethics and improvements in current practices. No known studies have specifically targeted this area of ethical recruitment.
Handle with care! Service contract termination as a service delivery task
Authors: Wetzel Hauke , Haenel Christina , Hess Alexandra Claudia 
Published: September 2022

Purpose
Profitability considerations lead service providers to terminate service contracts with low-value customers. However, customers targeted by service contract terminations often take revenge through negative word-of-mouth (NWOM). Presently, it is unclear how service contract termination initiatives prevent this harmful side effect. The purpose of this study is to compare the effectiveness of common service contract termination initiatives for reducing NWOM of customers whose service contracts are being cancelled. The study results provide guidance for minimizing the downside of service contract termination.

Design/methodology/approach
This study distinguishes between service contract termination initiatives common in practice (preannouncement, explanation, financial compensation, apology and support in finding an alternative provider). Drawing on a multi-industry survey of 245 customers who have experienced service contract terminations in real life, the authors estimate regression models to link perceived service contract termination initiatives to NWOM.

Findings
All else equal, only preannouncement and support in finding an alternative are effective to reduce NWOM. This study also shows that the right choice of service contract termination initiatives depends on the context of the termination. Making a preannouncement, offering an explanation and providing support in finding an alternative are more effective in reducing NWOM when these actions are aligned with the contextual factors of relationship duration and competitive intensity.

Research limitations/implications
This study shows that service contract termination needs to address several aspects of the service termination experience. The key implication for future research is that it matters in terms of NWOM how service contract terminations are performed.

Practical implications
This research identifies the service contract termination initiatives that are most effective to reduce NWOM after service contract termination in general and under consideration of the moderating roles of relationship duration and competitive intensity.

Originality/value
While most related studies have considered customer responses to the cancellation of other customers’ contracts, this study contributes to the scarce literature on the undesirable customer responses (such as NWOM) to the termination of their own contract. To the best of the authors’ knowledge, it is the first study in this emerging stream of research that accounts for the effects of process- and outcome-oriented contract termination initiatives on NWOM. To the best of the authors’ knowledge, it is also the first study to account for moderators of the effect of contract termination initiatives on NWOM, namely, relationship duration and competitive intensity.
The determinants of Muslim millennials’ visiting intention towards halal tourist attraction
Authors: Juliana Juliana , W. Mahri A. Jajang , Salsabilla Azkiya Rahmah , Muhammad Mumuh , Nusannas Iman Sidik 
Published: September 2022

Purpose
This study aims to investigate the relationship between destination image (DI), travel motivation, religiosity, Islamic attributes of destination and the Muslim millennial’s visiting intention to halal tourist attraction.

Design/methodology/approach
Using quantitative method, this study analyses responses to a questionnaire distributed to 200 respondents living in various province in Indonesia. To prove the hypothesis, the data collected are analysed using partial least square-structural equation modelling (PLS-SEM).

Findings
This study shows that there is significant relationship positively between travel motivation, religiosity, Islamic attributes of destination and Muslim millennials’ visiting intention to Lombok as halal tourist attraction. However, the DI shows insignificant impact on Muslim millennials’ visiting intention. The results confirm that travel motivation, religiosity and Islamic attributes of destination are highly important in encouraging the intention of Muslim millennials to visit halal tourist attraction.

Research limitations/implications
This study, however, has limitations. Firstly, the observation of this study is only aimed at Muslim millennials aged from 20 to 40 years. Secondly, the variables used in this study are only four variables.

Practical implications
To increase the visiting intention among Muslim millennials to halal tourism destination, the authorities should create and design the concept of halal tourism to attract Muslim millennials by considering the factors in this study. In addition, related parties need to improve halal infrastructure quality to boost halal tourism development. Indonesia is the biggest Muslim country in the world recently, the better promotion and campaign of halal tourism, the more Muslim millennials who are interested to visit halal tourism destination.

Originality/value
This study explores Muslim millennials’ visiting intention to halal tourist attraction in Indonesia. The study combines four important determinants affecting Muslim millennials to visit Lombok as one of the halal tourist attractions in Indonesia. In addition to that, literature explaining visiting intention in the context of prospective Muslim millennial is scarce.
Contribution of machine learning in continuous improvement processes
Authors: Mjimer Imane , Aoula Es-Saadia , Achouyab E.L. Hassan 
Published: September 2022

PurposeThe aim of this study is to predict one of the key performance indicators used to improve continually production systems using machine learning techniques known by the ability to teach the machine to perform complex things as opposed to simple statistical methods by giving this machine the historical dataset, according to the kind of machine learning the authors will use, the machine will be able to predict a new output data from the input data given by the user.Design/methodology/approachThis work is divided into six sections: In the first section, the state of art for OEE, machine learning, and regression models. In the second section, the methodology, followed by an experimental study conducted in an automotive company specialised in the manufacturing of manual transmissions.FindingsThe three models show a very high accuracy (higher than 99%), a comparison between these three models was done using three indicators, namely mean absolute error (MAE) mean square error (mean squared error and mean absolute percentage error which shows that the best model is the least angle followed by Bayesian Ridge and automatic relevance determination regression.Originality/valueAs the authors can see many works were done in the different production systems for prediction, the most relevant works were done to predict a parameter in the production system such as The prediction of part thickness in aluminium hot stamping process with partition temperature control the prediction of CO2 trapping performance the prediction of crop yield the prediction of lean manufacturing in automotive parts industry the contribution of the work will be to use the machine learning techniques to predict the key performance indicator “used to measure manufacturing efficiency” which is the overall equipment effectiveness used in the authors’ case to measure the improvement of the production system.
Impact of a collaboration revolving around virtual capacity evaluations
Authors: Factora Ronan , Hashmi Ardeshir Z. 
Published: September 2022

AbstractObjectiveTo assess the impact of virtual capacity assessments on access to medical care, community supports, and transitions to higher levels of care.Study SettingVirtual capacity evaluations of homebound suspected elder abuse/neglect/financial exploitation victims identified via exclusion criteria and initiated by Cuyahoga County adult protective services (APS) and conducted with Cleveland Clinic Geriatric Medicine.Study DesignA retrospective chart review was conducted in conjunction with APS using their database to determine the outcomes of individuals who underwent virtual capacity evaluation from May 2020 through September 2021. Variables collected included completion of a statement of expert evaluation, guardianship assignment, offering community services, transfer to a higher level of care, and establishment of primary care.Data Collection/ExtractionData were extracted from medical records and the APS database. Outcomes were measured as percentages.Principal FindingsFifty‐four individuals underwent evaluation. Statements of expert evaluation were completed in 38 cases (70%). Guardianship was assigned in 28 cases (52%). Community services were offered to 51 (89%). Thirty‐one (57%) remained at home. At baseline, only 23 (43%) were receiving primary care. Post evaluation, 44 (81%) were connected or reconnected to their medical provider.ConclusionOf individuals who underwent our virtual capacity evaluations, most were able to remain at home, offered community services for support, and linked to primary care.
Hexosamine pathway activation improves memory but does not extend lifespan in mice
Authors: Allmeroth Kira , Hartman Matías D. , Purrio Martin , Mesaros Andrea , Denzel Martin S. 
Published: September 2022

AbstractGlucosamine feeding and genetic activation of the hexosamine biosynthetic pathway (HBP) have been linked to improved protein quality control and lifespan extension. However, as an energy sensor, the HBP has been implicated in tumor progression and diabetes. Given these opposing outcomes, it is imperative to explore the long‐term effects of chronic HBP activation in mammals. Thus, we asked if HBP activation affects metabolism, coordination, memory, and survival in mice. N‐acetyl‐D‐glucosamine (GlcNAc) supplementation in the drinking water had no adverse effect on weight in males but increased weight in young females. Glucose or insulin tolerance was not affected up to 20 months of age. Of note, we observed improved memory in young male mice supplemented with GlcNAc. Survival was not changed by GlcNAc treatment. To assess the effects of genetic HBP activation, we overexpressed the pathway's key enzyme GFAT1 and a constitutively activated mutant form in all mouse tissues. We detected elevated levels of the HBP product UDP‐GlcNAc in mouse brains, but did not find any effects on behavior, memory, or survival. Together, while dietary GlcNAc supplementation did not extend survival in mice, it positively affected memory and is generally well tolerated.
Analysis of alcohol use, consumption of micronutrient and macronutrients, and liver health in the 2017–2018 National Health and Nutrition Examination Survey
Authors: Warner Jeffrey B. , Zirnheld Kara H. , Hu Huirong , Floyd Alison , Kong Maiying , McClain Craig J. , Kirpich Irina A. 
Published: September 2022

AbstractBackgroundAlcohol use is a major global healthcare burden that contributes to numerous adverse health outcomes, including liver disease. Many factors influence individual susceptibility to alcohol‐associated diseases, including nutritional factors. The objective of the current study was to examine inter‐relations among alcohol, dietary micronutrients and macronutrient consumption, and liver health by analyzing data from the 2017–2018 National Health and Nutrition Examination Survey (NHANES).MethodsBased on self‐reported alcohol consumption, NHANES respondents were assigned to one of four categories: never drinkers (lifetime abstainers), non‐drinkers (past‐year abstainers), moderate drinkers (1/2 drinks per day for females/males, respectively), and heavy drinkers (>1/>2 drinks per day for females/males, respectively, and/or frequent binge drinking). Survey‐weighted regression analyses (adjusted for gender, age, race, education, and body mass index) were performed to examine associations between alcohol intake, dietary, and liver health characteristics.ResultsIndividuals categorized as heavy drinkers were significantly younger, most often well‐educated males with low incidences of diabetes and other comorbidities. They consumed the most overall calories and various micronutrients, indicating a diet that was not necessarily nutrient poor. Neither moderate nor heavy drinkers had liver steatosis or fibrosis as measured by liver elastography, although heavy drinkers had modestly elevated plasma biomarkers of liver injury, including ALT, AST, and GGT, compared with the other groups.ConclusionsOur findings suggest that the category of heavy drinkers in the 2017–2018 NHANES consisted of generally healthy individuals with high‐energy intake and no evidence of liver steatosis or fibrosis. However, slightly increased plasma liver markers may indicate a risk of future progression to more advanced stages of liver disease over time in some individuals. Several limitations should be considered when interpreting these data, including the potential misclassification of drinking categories and the lack of standardized cutoff scores for fatty liver as assessed by elastography, among others.
Evaluation and validation of chemotherapy‐specific diarrhoea and histopathology in rats
Authors: Dahlgren David , Rosenqvist Evelina , Hellström Per M. , Nygren Peter , Kullenberg Fredrik , Peters Karsten , Sjöblom Markus , Lennernäs Hans 
Published: September 2022

AbstractChemotherapy‐induced mucositis is characterized by diarrhoea and villous atrophy. However, it is not well‐understood why diarrhoea arises, why it only occurs with some chemotherapeutics and how it is related to villus atrophy. The objectives in this study were to determine (i) the relationship between chemotherapy‐induced diarrhoea and villus atrophy and to (ii) establish and validate a rat diarrhoea model with clinically relevant endpoints. Male Wistar Han IGS rats were treated with saline, doxorubicin, idarubicin, methotrexate, 5‐fluorouracil, irinotecan or 5‐fluorouracil+irinotecan. After 72 h, jejunal tissue was taken for morphological, apoptotic and proliferative analyses, and faecal water content and change in body weight were determined. All treatments except methotrexate caused a similar reduction (≈42%) in villus height, but none of them altered mucosal crypt cell proliferation or apoptosis. Doxorubicin, idarubicin, irinotecan and 5‐fluorouracil+irinotecan caused body weight reduction, but only irinotecan and idarubicin caused diarrhoea. No direct correlation between diarrhoea and villus height or body weight loss was observed. Therefore, studies of the mechanisms for chemotherapy‐induced diarrhoea should focus on functional factors. Finally, the irinotecan and idarubicin diarrhoea models established in this study will be useful in developing supportive treatments of this common and serious adverse effect in patients undergoing chemotherapy.
The antecedents and consequences of stereotype threat in Parkinson's disease
Authors: Coundouris Sarah P. , von Hippel Courtney , Lehn Alexander C. , Henry Julie D. 
Published: September 2022

AbstractObjectiveThe aim of this study was to examine what factors make people with Parkinson's disease more susceptible to stereotype threat (i.e., the concern of being evaluated in line with negative group‐based stereotypes) and the clinical consequences of this.MethodForty‐nine people with Parkinson's disease completed a measure of stereotype threat, as well as measures that indexed potential antecedents and clinical consequences of stereotype threat.ResultsYounger age and greater communication difficulties emerged as significant predictors of stereotype threat. Higher stereotype threat was also associated with increased emotional distress.ConclusionsThese findings are an important first step in understanding stereotype threat in Parkinson's disease and may help to guide the development of intervention and educational efforts aimed at countering its effects.
Healthy masculinity construction: The influence of race, faith and athletics
Authors: Newman Tarkington J. , McCray Kristy , Lower‐Hoppe Leeann M. , Rockhill Carter , Ingram Donta , Ohanasian Justice , Simmons‐Horton Sherri 
Published: September 2022

AbstractDue to structural racism, young men living in urban cities—particularly Black and other youth of colour—are at risk for developing hegemonic, toxic masculine identities. However, through a positive youth development approach, sport can be used to promote healthy masculinity. This study explored the importance and meaning of masculinity, as well as influences of masculinity construction among 14 urban male youth who participated in a faith‐based sport program. Findings from semi‐structured interviews highlight the importance of a masculine identity; depict masculinity through key attributes (leadership, persistence, responsibility, confidence, strength) and underscore the intersectional influence of race, faith, athletics and adult role models.
Time‐lagged effects of habitat fragmentation on terrestrial mammals in Madagascar
Authors: Broekman Maarten J. E. , Hilbers Jelle P. , Schipper Aafke M. , Benítez‐López Ana , Santini Luca , Huijbregts Mark A. J. 
Published: September 2022

AbstractBiodiversity is severely threatened by habitat destruction. As a consequence of habitat destruction, the remaining habitat becomes more fragmented. This results in time‐lagged population extirpations in remaining fragments when these are too small to support populations in the long term. If these time‐lagged effects are ignored, the long‐term impacts of habitat loss and fragmentation will be underestimated. We quantified the magnitude of time‐lagged effects of habitat fragmentation for 157 nonvolant terrestrial mammal species in Madagascar, one of the biodiversity hotspots with the highest rates of habitat loss and fragmentation. We refined species’ geographic ranges based on habitat preferences and elevation limits and then estimated which habitat fragments were too small to support a population for at least 100 years given stochastic population fluctuations. We also evaluated whether time‐lagged effects would change the threat status of species according to the International Union for the Conservation of Nature (IUCN) Red List assessment framework. We used allometric relationships to obtain the population parameters required to simulate the population dynamics of each species, and we quantified the consequences of uncertainty in these parameter estimates by repeating the analyses with a range of plausible parameter values. Based on the median outcomes, we found that for 34 species (22% of the 157 species) at least 10% of their current habitat contained unviable populations. Eight species (5%) had a higher threat status when accounting for time‐lagged effects. Based on 0.95‐quantile values, following a precautionary principle, for 108 species (69%) at least 10% of their habitat contained unviable populations, and 51 species (32%) had a higher threat status. Our results highlight the need to preserve continuous habitat and improve connectivity between habitat fragments. Moreover, our findings may help to identify species for which time‐lagged effects are most severe and which may thus benefit the most from conservation actions.
Rare association of absent pulmonary valve syndrome, complete atrioventricular canal defect, double outlet right ventricle, right aortic arch, and aberrant right subclavian artery in a fetal case
Authors: Başar Eviç Zeynep , Doğan Yasemin , Şaşmazel Ahmet , Babaoğlu Kadir 
Published: September 2022

AbstractAbsent pulmonary valve syndrome (APVS) is a rare congenital cardiac anomaly. It is often associated with TOF, but may also be associated with an intact ventricular septum or, more rarely, with tricuspid atresia. It's combination with a complete atrioventricular septal defects and double outlet right ventricle has been reported extremely rare, even in the postnatal period. Herein, we report a 20‐week‐old fetus with a right aortic arch and an aberrant left subclavian artery with this rare combination. We report a case of a 20‐week fetus diagnosed having this rare combination of right aortic arch and aberrant left subclavian artery. The APVS with complete atrioventricular septal defects may represent another type of APVS.