bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and Assessment of Necroptosis-Related Genes in Clinical Prognosis and Immune Cells in Diffuse Large B-Cell Lymphoma
Authors: Zhang Qikai , Zhu Zongsi , Guan Jiaqiang , Zheng Cuiping 
Published: June 2022

BackgroundWith the unveiling of new mechanisms and the advent of new drugs, the prognosis of diffuse large B-cell lymphoma (DLBCL) becomes promising, but some patients still progress to the relapse or refractory stage. Necroptosis, as a relatively novel programmed cell death, is involved in the development of multiple tumors. There are no relevant studies on the prognostic significance of necroptosis in DLBCL to date.MethodsWe identified the differential necroptosis-related genes (NRGs) by comparing the DLBCL and normal control in GSE12195 and GSE56315 datasets. TCGA DLBC and GSE10846 containing clinical information and microarray expression profiling were merged as the entire cohort. We performed consensus clusters based on NRGs and two clusters were obtained. Kaplan–Meier (K-M) survival analysis, GSVA, GO, KEGG, and ssGSEA were used to analyze the survival, function, and immune microenvironment between two clusters. With LASSO and proportional hazard model construction, we identified differentially expressed genes (DEGs) between NRG clusters, calculated the risk score, established a prognostic model, and validated its value by calibration and ROC curves. The entire cohort was divided into the training and test cohort, and GSE87371 was included as an external validation cohort. K-M, copy number variation, tumor mutation burden, and drug sensitivity were also analyzed.ResultsWe found significant differences in prognosis between the two NRG clusters. Cluster A with a poor prognosis had a decreased expression of NRGs and a relatively suppressed immune microenvironment. GSVA analysis indicated that cluster A was related to the downregulation of the TGF-β signaling pathway and the activation of the Notch signaling pathway. The risk score had an accurate predictive ability. The nomogram could help predict the survival probability of DLBCL patients in the entire cohort and the external validation cohort. The area under the curve (AUC) of the nomogram, risk score, and International Prognostic Index was 0.723, 0.712, and 0.537, respectively. γ/δ T cells and Macrophage 1 cells decreased while Macrophage 2 cells and Natural Killer resting cells increased in the high-risk group. In addition, the high-risk group was more sensitive to the PI3K inhibitor and the PDK inhibitor.ConclusionWe explored the potential role of necroptosis in DLBCL from multiple perspectives and provided a prognostic nomogram for the survival prediction of DLBCL. Necroptosis was downregulated and was correlated with an immunosuppressed tumor microenvironment and poor prognosis in DLBCL. Our study may deepen the understanding and facilitate the development of new therapy targets for DLBCL.
Risk Factors for Short Stature in Children Born Small for Gestational Age at Full-Term
Authors: Ling Lan , Chen Ting , Zhang Xin-Hua , Pan Min-Hong , Gong Hai-Hong , Zhang Li-Na , Zhao Meng , Chen Xiao-Qing , Cui Shu-Dong , Lu Chao 
Published: June 2022

ObjectiveThis study aims to identify the risk factors associated with short stature in children born small for gestational age (SGA) at full-term.MethodsThis was a retrospective study. The subjects were full-term SGA infants who were followed up until the age of 2 years. The risk factors for short stature were identified with univariate and multivariate analyses.ResultsOf 456 full-term SGA children enrolled in this study, 28 cases had short stature at 2 years of age. A significant decrease in placental perfusion was found in the short children group with intravoxel incoherent motion (IVIM) technology, which was an advanced bi-exponential diffusion-weighted imaging (DWI) model of magnetic resonance imaging (MRI) (p = 0.012). Compared to non-short children born SGA at full-term, the short children group underwent an incomplete catch-up growth. Mothers who suffered from systemic lupus erythematosus were more likely to have a short child born SGA (p = 0.023). The morbidity of giant placental chorioangioma was higher in the short children group. The pulsatility index (PI), resistivity index (RI), and systolic-diastolic (S/D) ratio of umbilical artery were higher in the short children group than in the non-short control group (p = 0.042, 0.041, and 0.043). Multivariate analysis demonstrated that decrease of perfusion fraction (fp) in IVIM of placental MRI, chromosomal abnormalities, short parental height, and absence of catch-up growth were associated with a higher risk of short stature in children born SGA at full-term.ConclusionRisk factors for short stature in full-term SGA children at 2 years of age included a decrease of perfusion fraction fp in IVIM of placental MRI, chromosomal abnormalities, and short parental height.
Comprehensive Therapy for Infant Vascular Tumor Associated With Kasabach–Merritt Phenomenon—Single-Center Primary Experience
Authors: Sun Xiaoting , Xu Miao , Lv Kaiyang , Ma Xiaorong , Wu Liming , Ouyang Tianxiang 
Published: June 2022

ObjectiveTo introduce our single-center experience of infant vascular tumor associated with Kasabach–Merritt phenomenon (KMP) which received combined medicine treatment with intralesional laser photocoagulation (ILP) and sclerotherapy.MethodsA retrospective study was conducted using medical records of all children with a diagnosis of kaposiform hemangioendothelioma (KHE) or tufted angioma (TA) associated with KMP treated with medicine, intralesional laser photocoagulation (ILP), and sclerotherapy between February 2017 and November 2020. Clinical features, response to comprehensive therapy, and outcomes were recorded.ResultsA total of 23 patients including nine females (39%) and 14 males (61%) were identified. The mean age was 6.9 months (age range, 11 days−2 years) at the time of treatment. Nine children (39%) demonstrated sensitivity to single corticosteroid therapy; 14 children (61%) received combined therapy with intravenous Vincristine (VCR) and corticosteroid therapy. All children had at least two ILP and sclerotherapy performed, with a mean of 3.5 procedures (range: 2–6). Of these 14 children, only one experienced a relapse of thrombocytopenia and the remaining 13 children had no clinical symptoms recurred.ConclusionThe combined therapy modalities could induce a more rapid tumor response and resolution of KMP and decrease the rebound rates. This research presents a novel and safe multi-modality treatment for infant vascular tumors associated with KMP.
Machine Learning for Discovery of New ADORA Modulators
Authors: Puhl Ana C. , Gao Zhan-Guo , Jacobson Kenneth A. , Ekins Sean 
Published: June 2022

Adenosine (ADO) is an extracellular signaling molecule generated locally under conditions that produce ischemia, hypoxia, or inflammation. It is involved in modulating a range of physiological functions throughout the brain and periphery through the membrane-bound G protein-coupled receptors, called adenosine receptors (ARs) A1AR, A2AAR, A2BAR, and A3AR. These are therefore important targets for neurological, cardiovascular, inflammatory, and autoimmune diseases and are the subject of drug development directed toward the cyclic adenosine monophosphate and other signaling pathways. Initially using public data for A1AR agonists we generated and validated a Bayesian machine learning model (Receiver Operator Characteristic of 0.87) that we used to identify molecules for testing. Three selected molecules, crisaborole, febuxostat and paroxetine, showed initial activity in vitro using the HEK293 A1AR Nomad cell line. However, radioligand binding, β-arrestin assay and calcium influx assay did not confirm this A1AR activity. Nevertheless, several other AR activities were identified. Febuxostat and paroxetine both inhibited orthosteric radioligand binding in the µM range for A2AAR and A3AR. In HEK293 cells expressing the human A2AAR, stimulation of cAMP was observed for crisaborole (EC50 2.8 µM) and paroxetine (EC50 14 µM), but not for febuxostat. Crisaborole also increased cAMP accumulation in A2BAR-expressing HEK293 cells, but it was weaker than at the A2AAR. At the human A3AR, paroxetine did not show any agonist activity at 100 µM, although it displayed binding with a Ki value of 14.5 µM, suggesting antagonist activity. We have now identified novel modulators of A2AAR, A2BAR and A3AR subtypes that are clinically used for other therapeutic indications, and which are structurally distinct from previously reported tool compounds or drugs.
Fitness Apps, Live Streaming Workout Classes, and Virtual Reality Fitness for Physical Activity During the COVID-19 Lockdown: An Empirical Study
Authors: Liu Ru , Menhas Rashid , Dai Jianhui , Saqib Zulkaif Ahmed , Peng Xiang 
Published: June 2022

BackgroundPhysical activity is an essential need of the human body that helps improve the physical fitness of an individual and creates a positive impact on overall wellbeing. Smartphone applications play an essential role in providing several benefits to consumers by offering various capabilities in terms of health and fitness.COVID-19 preventive measures shut down public places, and people cannot go to the gym and parks for physical activity. Smart applications for physical activity are an effective way to keep active while staying at home.ObjectiveThe objective of the present study was to assess the mediating role of the e-platforms physical activity among the Chinese people in China during the COVID-19 lockdown.MethodThe participants in this study were Chinese citizens living in home isolation during the early stages of the epidemic in China. The primary data was collected via an online survey using a convenience sample strategy in accordance with the study purpose. The collected data were cleaned by using the SPSS-25 statistical software. SmartPLS 3.0 software was used to investigate the suggested study framework utilizing the structural equation modeling technique.ResultsDescriptive statistics shows that the ratio of gender includes 49% (n = 2,626) male and 51% females in the entire sample. SEM results show that all hypotheses (H1: β = 0.497, T = 43.068, P = &lt;0.001; H2: β = 0.498, T = 41.078, P = &lt;0.001; H3: β = 0.498, T = 41.078, P = &lt;0.001; H4: β = 0.471, T = 39.103, P = &lt;0.001; H5: β = 0.468, T = 42.633, P = &lt;0.001; H6: β = 0.251, T = 11.212, P = &lt;0.001; H7: β = 0.367, T = 16.032, P = &lt;0.001; H8: β = 0.170, T = 13.750, P = &lt;0.001; H9: β = 0.125, T = 10.604, P = &lt;0.001; H10: β = 0.173, T = 14.842, P = &lt;0.001) were statistically confirmed.ConclusionIn COVID-19, when there are limited physical activity resources, smart applications play an essential role as an alternative to gyms and change people's perspective regarding the adoption of health and fitness. Smart applications have made exercise and physical activity accessible and convenient to adopt.
A Case Study of Co-production Within a Mental Health Recovery College Dementia Course: Perspectives of A Person With Dementia, Their Family Supporter and Mental Health Staff
Authors: West Juniper , Birt Linda , Wilson Danielle , Mathie Elspeth , Poland Fiona 
Published: June 2022

BackgroundUndertaking co-production as a power-sharing way to improve mental health dementia services remains uncommon, suggesting opportunities to apply knowledge from lived experience of people with dementia, may often be missed. One barrier is stigma, assuming people with progressive cognitive impairment cannot manage this level of participation, support peers nor offer a “valid” perspective.PurposeThis paper shares knowledge gained from a service evaluation that explored various experiences of a person with dementia, their family supporter and mental health staff, involved in co-producing a course about “living well” with dementia, within a mental health Recovery College.DesignA qualitative, case study approach used semi-structured interviewing and inductive thematic analysis.FindingsCo-production activities generated a shared sense of positivity, pride and privilege, highlighting positive effects in breaking down the “them and us” barriers common in traditional healthcare professional-service user relationships. Each individual had both something to offer and something to gain during the process. Staff identified challenges in the co-production process; in that balancing all the voices during meetings could be complex at times, and the process overall required considerable time commitment.ConclusionTaking part in co-production at an appropriate level and with peer support is a relational activity seen to be valuable in powerfully, yet gently, challenging stigma and assumptions around dementia. Findings show that while the process of co-production requires time and dedication, there is overall value in involving people living with dementia both in co-production and in peer support. This provided a straightforward and beneficial means to inclusively improve post-diagnosis support and care quality within a memory service.
A Heart Rate Based Algorithm to Estimate Core Temperature Responses in Elite Athletes Exercising in the Heat
Authors: de Korte Johannus Q. , Veenstra Bertil J. , van Rijswick Mark , Derksen Eline J. K. , Hopman Maria T. E. , Bongers Coen C. W. G. , Eijsvogels Thijs M. H. 
Published: June 2022

PurposeNon-invasive non-obtrusive continuous and real-time monitoring of core temperature (Tc) may enhance pacing strategies, the efficacy of heat mitigation measures, and early identification of athletes at risk for heat-related disorders. The Estimated Core Temperature (ECTemp™) algorithm uses sequential heart rate (HR) values to predict Tc. We examined the validity of ECTemp™ among elite athletes exercising in the heat.Methods101 elite athletes performed an exercise test in simulated hot and humid environmental conditions (ambient temperature: 31.6 ± 1.0°C, relative humidity: 74 ± 5%). Tc was continuously measured using a validated ingestible telemetric temperature capsule system. In addition, HR was continuously measured and used to compute the estimated core temperature (Tc−est) using the ECTemp™ algorithm.ResultsAthletes exercised for 44 ± 10 min and n = 5,025 readouts of Tc (range: 35.8–40.4°C), HR (range: 45–207 bpm), and Tc−est (range: 36.7–39.9°C) were collected. Tc−est demonstrated a small yet significant bias of 0.15 ± 0.29°C (p &lt; 0.001) compared to Tc, with a limit of agreement of ±0.45°C and a root mean square error of 0.35 ± 0.18°C. Utilizing the ECTemp™ algorithm as a diagnostic test resulted in a fair to excellent sensitivity (73–96%) and specificity (72–93%) for Tc−est thresholds between 37.75 and 38.75°C, but a low to very-low sensitivity (50–0%) for Tc−est thresholds &gt;39.0°C, due to a high prevalence of false-negative observations.ConclusionECTemp™ provides a valuable and representative indication of thermal strain in the low- to mid-range of Tc values observed during exercise in the heat. It may, therefore, be a useful non-invasive and non-obtrusive tool to inform athletes and coaches about the estimated core temperature during controlled hyperthermia heat acclimation protocols. However, the ECTemp™ algorithm, in its current form, should not solely be used to identify athletes at risk for heat-related disorders due to low sensitivity and high false-negative rate in the upper end of the Tc spectrum.
Treatment Effects of Intra-Articular Allogenic Mesenchymal Stem Cell Secretome in an Equine Model of Joint Inflammation
Authors: Kearney Clodagh M. , Khatab Sohrab , van Buul Gerben M. , Plomp Saskia G. M. , Korthagen Nicoline M. , Labberté Margot C. , Goodrich Laurie R. , Kisiday John D. , Van Weeren P. R. , van Osch Gerjo J. V. M. , Brama Pieter A. J. 
Published: June 2022

BackgroundAllogenic mesenchymal stem cell (MSC) secretome is a novel intra-articular therapeutic that has shown promise inin vitroand small animal models and warrants further investigation.ObjectivesTo investigate if intra-articular allogenic MSC-secretome has anti-inflammatory effects using an equine model of joint inflammation.Study DesignRandomized positively and negatively controlled experimental study.MethodIn phase 1, joint inflammation was induced bilaterally in radiocarpal joints of eight horses by injecting 0.25 ng lipopolysaccharide (LPS). After 2 h, the secretome of INFy and TNFα stimulated allogeneic equine MSCs was injected in one randomly assigned joint, while the contralateral joint was injected with medium (negative control). Clinical parameters (composite welfare scores, joint effusion, joint circumference) were recorded, and synovial fluid samples were analyzed for biomarkers (total protein, WBCC; eicosanoid mediators, CCL2; TNFα; MMP; GAGs; C2C; CPII) at fixed post-injection hours (PIH 0, 8, 24, 72, and 168 h). The effects of time and treatment on clinical and synovial fluid parameters and the presence of time-treatment interactions were evaluated. For phase 2, allogeneic MSC-secretome vs. allogeneic equine MSCs (positive control) was tested using a similar methodology.ResultsIn phase 1, the joint circumference was significantly (p&lt; 0.05) lower in the MSC-secretome treated group compared to the medium control group at PIH 24, and significantly higher peak synovial GAG values were noted at PIH 24 (p&lt; 0.001). In phase 2, no significant differences were noted between the treatment effects of MSC-secretome and MSCs.Main LimitationsThis study is a controlled experimental study and therefore cannot fully reflect natural joint disease. In phase 2, two therapeutics are directly compared and there is no negative control.ConclusionsIn this model of joint inflammation, intra-articular MSC-secretome injection had some clinical anti-inflammatory effects. An effect on cartilage metabolism, evident as a rise in GAG levels was also noted, although it is unclear whether this could be considered a beneficial or detrimental effect. When directly comparing MSC-secretome to MSCs in this model results were comparable, indicating that MSC-secretome could be a viable off-the-shelf alternative to MSC treatment.
Variation in suspected cancer referral pathways in primary care: comparative analysis across the International Benchmarking Cancer Partnership
Authors: Lynch Charlotte , Harrison Samantha , Emery Jon D , Clelland Cathy , Dorman Laurence , Collins Claire , Johansen May-Lill , Lawrenson Ross , Surgey Alun , Weller David , Jarbøl Dorte Ejg , Balasubramaniam Kirubakaran , Nicholson Brian D 
Published: June 2022

BackgroundInternational variations in cancer outcomes persist and may be influenced by differences in the accessibility and organisation of cancer patient pathways. More evidence is needed to understand to what extent variations in the structure of primary care referral pathways for cancer investigation contribute to differences in the timeliness of diagnoses and cancer outcomes in different countries.AimTo explore the variation in primary care referral pathways for the management of suspected cancer across different countries.Design and settingDescriptive comparative analysis using mixed methods across the International Cancer Benchmarking Partnership (ICBP) countries.MethodSchematics of primary care referral pathways were developed across 10 ICBP jurisdictions. The schematics were initially developed using the Aarhus statement (a resource providing greater insight and precision into early cancer diagnosis research) and were further supplemented with expert insights through consulting leading experts in primary care and cancer, existing ICBP data, a focused review of existing evidence on the management of suspected cancer, published primary care cancer guidelines, and evaluations of referral tools and initiatives in primary care.ResultsReferral pathway schematics for 10 ICBP jurisdictions were presented alongside a descriptive comparison of the organisation of primary care management of suspected cancer. Several key areas of variation across countries were identified: inflexibility of referral pathways, lack of a managed route for non-specific symptoms, primary care practitioner decision-making autonomy, direct access to investigations, and use of emergency routes.ConclusionAnalysing the differences in referral processes can prompt further research to better understand the impact of variation on the timeliness of diagnoses and cancer outcomes. Studying these schematics in local contexts may help to identify opportunities to improve care and facilitate discussions on what may constitute best referral practice.
Hacia una historia crítica de la mirada. Repensando el uso de la imagen dentro de las ciencias sociales
Authors: Flores Nava Fabiola Jesavel 
Published: June 2022

En este artículo, se rescata el discurso crítico como elemento fundamental para sustentar lo que se ha denominado Historia crítica de la mirada. Aquí no se intenta establecer un simple método de interpretación de la imagen, sino que, por el contrario, se pretende rescatar el uso de la imagen como parte fundamental de un amplio análisis capaz de contener dentro de sí memorias y expresiones sociales que permitan exponer las contradicciones imperantes en la vida moderna, una vida que obliga o vuelve indispensable el ocultamiento del funcionamiento de la realidad para que en la práctica cotidiana permanezcan vigentes las formas institucionalizadas de la sociedad. Por ello planteamos que los juicios, las ideologías y preferencias de los que operan el aparato, los que crean la imagen, los que la interpretan, así como de los que encargan las imágenes, etc., no son neutrales, sino que están inmersas en un mundo complejo, contradictorio y construido sobre dinámicas cotidianas que reproducen y favorecen una práctica material determinada, muchas veces acorde con el discurso dominante.
Pengaruh Kepemimpinan Transformasional, Partisipasi, Komunikasi dan Motivasi Kerja Terhadap Komitmen untuk Perubahan (Studi Pada Dosen dan Pegawai Akademi Maritim Sapta Samudra Padang)
Authors: Marjohan Marjohan 
Published: June 2022

Tujuan dari penelitian ini adalah untuk menentukan faktor-faktor yang mempengaruhi komitmen untuk perubahan pada dosen dan pegawai di Akademi Maritim Sapta Samudra Padang. Komitmen untuk perubahan adalah faktor kunci keberhasilan di dalam organisasi dari perubahan organisasi. Oleh karena itu upaya untuk menemukan faktor-faktor yang mempengaruhinya menjadi hal yang penting. Penelitian ini menggunakan 35 responden. Analisis regresi linier berganda dengan menggunakan SPSS versi 17. Penelitian ini menunjukkan bahwa ada tiga variabel yang signifikan dan positif berpengaruh terhadap komitmen untuk perubahan. Sedangkan variabel motivasi kerja tidak terdapat pengaruh yang signifikan dengan komitmen untuk berubah. Secara praktis, hasil penelitian ini dapat digunakan oleh pimpinan Akademi Maritim Sapta Samudra Padang untuk menjalankan komitmen untuk berubah dengan meningkatkan kepemimpinan transformasional, partisipasi, dan komunikasi. Sementara secara teoritis penelitian ini memberi kontribusi prilaku terencana.