bluuebunny/crossref_metadata_2025_split huggingface dataset:
IL-1β and TNF-α play an important role in modulating the risk of periodontitis and Alzheimer’s disease
Authors: Wang Rachel Pei-Hsuan , Huang Jianpan , Chan Kannie Wan Yan , Leung Wai-Keung , Goto Tetsuya , Ho Yuen-Shan , Chang Raymond Chuen Chung 
Published: September 2022

Abstract
Background
Systemic activation of the immune system can exert detrimental effects on the central nervous system. Periodontitis, a chronic disease of the oral cavity, is a common source of systemic inflammation. Neuroinflammation might be a result of this to accelerate progressive deterioration of neuronal functions during aging or exacerbate pre-existing neurodegenerative diseases such as Alzheimer’s disease. With advancing age, the progressive increase in the body’s pro-inflammatory status favors the state of vulnerability to both periodontitis and Alzheimer’s disease. In the present study, we sought to delineate the roles of cytokines in the pathogenesis of both diseases.
Methods
To examine the impacts of periodontitis on the onset and progression of Alzheimer’s disease, six-month-old female 3×Tg-AD mice and their age-matched non-transgenic mice were employed. Periodontitis was induced using two different experimental models: bacterial-induced periodontitis and ligature-induced periodontitis. To delineate the roles of pro-inflammatory cytokines in the pathogenesis of periodontitis and Alzheimer’s disease, interleukin (IL)-1β and tumor necrosis factor (TNF)-α were also injected into the buccal mandibular vestibule of mice.
Results
Here, we show that IL-1β and TNF-α were two of the most important and earliest cytokines upregulated upon periodontal infection. The systemic upregulation of these two cytokines promoted a pro-inflammatory environment in the brain contributing to the development of Alzheimer’s disease-like pathology and cognitive dysfunctions. Periodontitis-induced systemic inflammation also enhanced brain inflammatory responses and subsequently exacerbated Alzheimer’s disease pathology and cognitive impairment in 3×Tg-AD mice. The role of inflammation in connecting periodontitis to Alzheimer’s disease was further affirmed in the conventional magnetization transfer experiment in which increased glial responses resulting from periodontitis led to decreased magnetization transfer ratios in the brain of 3×Tg-AD mice.
Conclusion
Systemicinflammation resulting from periodontitis contributed to the development of Alzheimer’s disease tau pathology and subsequently led to cognitive decline in non-transgenic mice. It also potentiated Alzheimer’s disease pathological features and exacerbated impairment of cognitive function in 3×Tg-AD mice. Taken together, this study provides convincing evidence that systemic inflammation serves as a connecting link between periodontitis and Alzheimer’s disease.
Effects of pipelle endometrial biopsy on pretreatment tumor sampling for advanced ovarian, fallopian tube, and primary peritoneal cancers
Authors: Tamura Ryo , Kitani Yohei , Takahashi Kotaro , Yamaguchi Masayuki , Nishikawa Nobumichi , Kawasaki Takashi , Kikuchi Akira 
Published: September 2022

Abstract
Background
There have been several reports of ovarian cancer cells being detected on endometrial cytology. Recently, pipelle endometrial biopsy has been widely performed as an effective, minimally invasive, and low-cost test for endometrial diseases. However, the effectiveness of pretreatment pipelle endometrial biopsy in the ovarian, fallopian tube, and peritoneal cancers remains unexplored.
Methods
We performed pretreatment pipelle endometrial biopsy for 90 patients with ovarian, fallopian tube, and primary peritoneal cancers from January 2014 to November 2021. We retrospectively analyzed the association between the results of pipelle endometrial biopsy and clinicopathological data.
Results
25/61 (41.0%) cases with clinical stage II or higher were diagnosed with pipelle endometrial biopsy-positive (Pipelle-positive) and Pipelle-positive was not observed in 29 cases with clinical stage I. Pipelle-positive had significantly more high-grade serous carcinomas (p = 0.005), positive peritoneal cytology (p < 0.001), positive endometrial cytology (p < 0.001), and positive cervical cytology (p < 0.001) than pipelle endometrial biopsy-negative cases. In 23 Pippele-positive patients, we confirmed surgical pathology, and 17/23 (74.0%) had a completely same diagnosis with pipelle endometrial biopsy. Conversely, 6/23 (26.0%) showed a minor diagnostic discrepancy between pipelle endometrial biopsy and surgical pathology. Companion diagnostic tests (myChoice CDx and microsatellite instability test) were performed using pipelle endometrial biopsy samples in four primarily inoperable high-grade serous carcinoma patients and all tests were evaluable.
Conclusion
Pipelle endometrial biopsy is useful as a minimally invasive tumor sampling method for some advanced ovarian, fallopian tube, and peritoneal cancers. It may allow for prompt histological diagnosis and initiation of chemotherapy while collecting tumor tissue for genetic testing in primarily inoperable patients.
Pre-analytical Stability of Some Biochemical Analytes in Horse and Cattle
Authors: Megerssa Yoseph Cherinet 
Published: September 2022

Abstract
OBJECTIVE
Pre-analytical phase in laboratory testing is the serious part of analytical process which has impact on laboratory results. In these regard time delays between collection of samples and analysis are unavoidably common and samples are keep stored in refrigerator which might have effect on the accuracy of results.
RESULTS
Serum samples were obtained from 20 apparently healthy horse and cattle and aliquots of serum were divided in to three tubes. Two tubes (T1 and T2) were stored at -20°C for one and two months respectively while the other used for base line (T0) analysis. Total cholesterol, Triglycerides, Total protein, albumin and Urea were the tested analytes. Analysis of variance for selected analytes shown that there were no significant (P>0.05) differences in the mean Total Cholesterol, Total Protein, Albumin, Urea and Triglycerides values in T1 and T2 post storage, when compared to the baseline T0. The study also examined the changes in terms of total allowable error TEa (%) adopted from the American society of veterinary clinical pathology (ASVCP) guidelines and found that total observed error TEo (%) for the specified analytes was within the TEo(%). The results indicated that all analyses were stable up to 2 months when stored at -20°C; however a stability studies at different storage temperature, time intervals, and laboratory methods is recommended to fully validate their stability.
Indocyanine Green-enhanced Fluorescence to Assess Bowel Perfusion During Robotic-assisted Rectal Surgery Journal: International Journal of Colorectal Disease
Authors: Alawfi Homoud Ghazi , Yang Seung Yoon , Alessa Mohammed Yousef , Helmi Hadeel , Sakr Ahmad , Kim Nam Kyu 
Published: September 2022

Abstract
Purpose: Anastomotic leakage following rectal surgery is a major complication, which may occur due to insufficient vascular supply. We aimed to evaluate the feasibility and efficacy of using indocyanine green fluorescence angiography for intraoperative assessment of vascular anastomotic perfusion in robotic low anterior resection.
Methods: This was a retrospective case-control study that included consecutive patients undergoing robotic low anterior resection for rectal cancer between March 2017 and February 2019. Intra-operative use of indocyanine green fluorescence for assessment of bowel perfusion was performed in patients undergoing rectal surgery after April 2018. Those who underwent the surgery before that comprised the control group. The primary outcome was the occurrence of anastomotic leakage among the two groups.
Results:Each group included 48 patients. There were no significant differences between the two groups in terms of demographic data or tumor characteristics. The planned anastomotic site was revised in 1/48 patients who received indocyanine green fluorescence based on the surgeon’s subjective finding of a hypo-perfused distal segment. Postoperative anastomotic leakage was confirmed clinically and radiologically in one patient (2.8%) in the indocyanine green-group compared to two patients (4.16%) in the control group.
Conclusion: Indocyanine green fluorescence may be considered a useful intraoperative tool for assessment of vascular perfusion of bowel during robotic rectal surgery. Change in the site of resection and/or anastomosis may be indicated, possibly affecting the incidence of anastomotic leakage.
Learning Target Point Seeking Weights Spatial-Temporal Regularized Correlation Filters for Visual Tracking
Authors: Jiang Wen-Tao , Wang Zi-Min , Zhang Sheng-Chong , Zhou ZI-Qi 
Published: September 2022

Abstract
Spatial-temporal regularized correlation filtering (STRCF) is an effective tracking algorithm. Still, problems such as target occlusion, background clutter and out of view caused by target movement are inevitable in the running process. This paper proposes a Target Point Seeking Weights Spatial-Temporal Regularized Correlation Filters (TP-STRCF) to tackle target loss. The alternating direction method of multipliers (ADMM) is used to simplify the spatial-temporal regularized term in the objective function, obtain the optimal solution of the filter and auxiliary factor, reduce the algorithm's complexity, and realize the adaptability of time and space. The target weight response model is obtained through the target point finding weight, the target motion information is extracted, the target motion state and motion trajectory are predicted, and the interference information such as the algorithm's ability to identify the target and the ability to distinguish the target is enhanced. With the help of the weighted least squares method, the maximum response value of the weight is obtained, and the target position is determined. Experiments show that, compared with other mainstream correlation filtering algorithms, the proposed algorithm has higher tracking accuracy and stronger robustness under complex conditions.
Applications of plastic waste materials in bituminous mixes pavement for increasing the strengthen of pavement materials
Authors: Pallavi M. Lalitha , Dey Subhashish , Veerendra G.T.N. 
Published: September 2022

Abstract
The yearly production of plastic garbage is rising in the current environment as a result of the fast population rise. Recycling and reusing plastic trash is essential for sustainable development. The need of the hour is to utilize waste polythene for various supporting reasons since it is not biodegradable. These materials are made of polymers like polyethylene, polypropylene, and polystyrene. Due to the enhanced performance and elimination of the environmental issue, adding plastic waste to flexible pavement has emerged as a desirable choice. A composite material known as bituminous concrete (BC) is often utilized in construction projects such as road paving, airport terminals, stopover areas, etc. It includes mineral aggregate and black top or bitumen, which are combined, laid down in layers, and then compacted. The bituminous mixture in this research article was combined with plastic to use a chemical stabilizer. The ideal bitumen content is replaced by 0, 15, 27, and 36% plastic, as well as the bitumen's weight, stability, and Marshall values, to create hypothermal. A linear scale is used to compare the flow rates to the bituminous mixture. There have been several studies on the addition of trash to bituminous mixes, but this is focused on the use of plastic waste as a modification in a bitumen binder for flexible pavement. According to research, bituminous mixes containing up to 4 percent plastic waste are excellent for sustainable development.
Application of vector flow mapping technique in the assessment of right ventricular systolic function in patients with atrial fibrillation and normal left ventricular ejection fraction
Authors: Huang Fuqin , Wang Xing , Zhao Lina , Li Sha , Liao Chunyan , Yuan Yao , Li Aiyue , Zeng Weidan , Cao Hongjuan , Zhang Bei 
Published: September 2022

Abstract
Objective To evaluate the variation characteristics of systolic right intraventricular pressure differences (IVPDs) and right intraventricular pressure gradients (IVPGs) in patients with atrial fibrillation(AF) and normal left ventricular ejection fraction (LVEF) by relative pressure imaging (RPI) based on vector flow mapping(VFM),and to investigate their application value in right ventricular function in patients with normal LVEF AF.
Methods Thirty-one patients with normal LVEF AF were selected as normal LVEF AF group，and twenty healthy volunteers were selected as control group.Clinical data and echocardiographic images were collected, and conventional left and right ventricular diameter, area, volume and systolic function parameters were measured.IVPDs and IVPGs in isovolumic contraction（IC）, early systole(ES) and late systole(LS) were obtained by the VFM technique.The correlation between the IVPDs and IVPGs in the three systolic time phases and the conventional ultrasound parameters was analyzed between the normal LVEF AF group and the control group.
Results The systolic IVPDs and IVPGs in the normal LVEF AF group were significantly lower than those in the control group. Correlation analysis showed that ES-IVPGs and IC-IVPGs in patients with normal LVEF AF were positively correlated (P<0.05), and LS-IVPDs and LS-IVPGs were negatively correlated with ES-IVPDs (P<0.01).Left atrial end-diastolic volume (LAEDV) and left atrial end-systolic volume (LAESV) were negatively correlated with IC-IVPGs (P<0.01). The intraobserver correlation coefficients were 0.939, 0.944, and 0.901 for IC-IVPDs, ES-IVPDs, and LS-IVPDs, respectively; and the interobserver correlation coefficients were 0.854, 0.954, and 0.884, respectively.
Conclusions The patients with normal LVEF AF had a systolic right ventricular hemodynamics disorder, resulting in decreased IVPDs and IVPGs in each systolic phase. With a more pronounced decrease in IVPGs during IC which can be used as a potential new index to assess right ventricular hemodynamic changes in patients with normal LVEF AF.
Association of Polymorphic Gene Variants of Xenobiotic Biotransformation Enzymes with Lung Tissue Destruction in Tuberculosis Patients
Authors: Alymenko M. A. , Valiev R. Sh. , Valiev N. R. , Polonikov A. V. , Tragira I. N. , Sheenkov N. V. 
Published: September 2022

The objective: to investigate the association of polymorphic gene variants of xenobiotic biotransformation enzymes (NAT2, CYP2E1, ABCB1, GSTM1, GSTT1) and cytokines (IL1B, IL-4, IL-10, TNF) with lung tissue destruction in pulmonary tuberculosis patients.Subjects and Methods. An ethnically homogeneous population sample of unrelated individuals of Slavic nationalities (mainly Russians) living in Kursk Oblast suffering from new pulmonary tuberculosis was examined.Results. New pulmonary tuberculosis with no destructive changes in the lungs was associated with the EE genotype of the GSTM1 gene (p < 0.0001) in 89.5%, while the DD genotype of the GSTM1 gene (p < 0.0001) was associated with the presence of destructive changes in the lungs in 56.1% of cases; the dominant GG genotype of the TNF-α gene was associated with destructive changes in the lungs in 93.3% (p = 0.027), and the recessive GA genotype of the TNF-α gene was associated with no destructive changes in the lungs in 71.9% (p = 0.027).
Analysis of Management of Patients with COVID-19 in a Temporary Infectious Diseases Hospital Deployed on the Facilities of Republican TB Dispensary, the Republic of Tatarstan
Authors: Vizel A. A. , Shayakhmetova S. A. , Alekseev A. P. , Vizel I. Yu. , Nikitin A. V. , Galimov A. A. 
Published: September 2022

COVID-19 is a viral infection and its prevalence requires investigation of different co-morbid conditions including tuberculosis, as well as operation of temporary infectious diseases hospitals (TIDH) for the treatment of COVID-19 deployed on the facilities of anti-tuberculosis institutions.The objective: analysis of follow-up and treatment of patients with COVID-19 in the temporary infectious diseases hospitals deployed on the facilities of anti-tuberculosis institutions, and assessment of the impact of various medications and treatment methods on patient lethality including in those with co-morbid active tuberculosis.Subjects and Methods. Data of 143 patients (39.2% men and 60.8% women) aged 31 to 93 years old were collected and systematized using the COVIZ software (developed in the Republic of Tatarstan to analyze COVID-19 patient management, C#). A positive result of polymerase chain reaction for coronavirus was found in 88.1% of patients on admission and in 89.5% of patients over the entire follow-up period. 120 (83.9%) patients recovered, and 23 (16.1%) died.Results. Patients with different outcomes did not differ by age, sex, body mass index, and respiratory clinical manifestations. Oral anticoagulants prescribed before hospitalization, oral glucocorticosteroids prescribed on day 12 of the disease or later had a significant favorable effect on the COVID-19 outcome. Among those who received olokizumab, cure was achieved in 81.8%.The effect of anticytokine therapy was higher among patients previously vaccinated with the anti-COVID vaccine who had no concomitant pathology. Antimicrobial therapy generally had no effect on the COVID-19 outcomes, and early administration of these drugs increased the likelihood of a lethal outcome. Among 173 tuberculosis patients who died of other causes than tuberculosis, only 5 (2.9%) died of COVID-19. Tuberculosis patients followed up in the temporary infectious diseases hospital deployed on the facilities of Republican TB Dispensary were characterized by a relatively favorable course of this viral infection. The fact that those patients had been detected in a TB hospital and their treatment had been started early provided a positive impact.Conclusion. This study has demonstrated the potential for a rapid and appropriate public health response to emerging unpredictable infectious threats.
Microrna-96 In Human Cancers
Authors: Wu Yiwen , Xie Zhizhong , Deng Sijun , Xia Yiniu , Lei Xiaoyong , Yang Xiaoyan 
Published: September 2022

Abstract:
MicroRNAs (miRNAs) are small non-coding RNAs 19-25 nucleotides in size involved
in gene regulation and diverse processes in tumor cells. Abnormal expression of miRNAs is closely
related to carcinogenesis. MiR-96 is a salient cancer-related miRNA in a variety of tumors. Recent
evidence indicates that miR-96 has been observed to be wrapped in exosome and associated
with drug resistance or radio-chemosensitivity in cancers. miR-96 is also inextricably linked with
the competing endogenous RNAs (ceRNAs) in cancers. Notably, miR-96 plays both a tumor suppressor
role and plays a carcinogenic role in the same cancers. This review summarizes the critical
role of cancer-related miR-96 in drug resistance or radio-chemosensitivity and ceRNA mechanisms
of miR-96 in cancer. And we innovatively propose that miR-96 has a yin-yang effect in cancers.
Based on these several major roles of miR-96 in cancer as described above, we speculate that the
abnormal expression of miR-96 is likely to be novel potential therapeutic targets in cancers. It is
expected to solve the treatment problems such as low chemoradiotherapy sensitivity, poor prognosis
quality of life and easy recurrence in cancer patients.
The Importance of mir-491-5p in Various Cancers
Authors: Fakeri Mahsa , Armandzadeh Seyed Masoud , Olyaei Samad Sadigh , Foruzandeh Zahra , Alivand Mohammad Reza 
Published: September 2022

Abstract:
MicroRNAs are non-coding ribonucleic acids that are evolutionarily protected.
MiRNAs control the expression of genes after transcription by mRNA decomposition
or the inhibition of their translation. These molecular structures control physiological
and pathological processes; therefore, many of them can play vital roles as oncogenes
or tumor inhibitors. Besides, the occurrence of various mutations in miRNAs
can lead to cancer. In this review article, we want to peruse the role of miR-491-5p in
various cancers. In recent years, many experiments and studies have been performed
on the involvement of miR-491-5p in cancer, invasion, and cell metastasis. Metastasis
is an event that makes cancer more advanced and harder to treat. When cancer is
invasive, the cancer cells invade nearby tissues or other organs and develop cancer.
Tumor studies have shown that miR-491-5p can inhibit cell growth, invasion, and metastasis.
Thus, expression enhancement of miR-491-5p disrupts cell migration and
improves cancer.
Novel Persian Gulf Aminated Alginate Derivatives from Sargassum
Bovaneum: Synthesis and Characterization
Authors: Khodayar Shokouh , Shushizadeh Mohammad Reza , Tahanpesar Elham , Makhmalzadeh Behzad Sharif , Sanaeishoar Haleh 
Published: September 2022

Aims:
The aim of the study was to extract and characterize alginate from a new source, conduct
synthesis and characterization of novel aminated alginate derivatives, make a comparison of physicochemical
properties of extracted sodium alginate with its aminated derivatives, and investigate the
effect of diamines chain length on physicochemical properties of newly synthesized derivatives.

Background:
Alginate is a natural biopolymer found in marine brown seaweeds. Alginate is widely
used in the industry due to its features, such as gelling ability, biocompatibility, biodegradability, hydrophilicity,
and non-toxicity. Alginate has two types of functional groups, free hydroxyl and carboxyl
groups, which can be modified.

Methods:
In this study, sodium alginate was extracted from sargassum boveanum in basic media and
characterized by physical and spectral properties. In order to prepare alginate precursors with clickable
groups for hydrogel cross-linking application, extracted sodium alginate was further treated with two
different diamines, diaminoheptane (DAH) and diaminopropane (DAP), in the presence of 1-ethyl-3-
(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to
form amide linkages on the alginate backbone and synthesise aminated derivatives. The products were
characterized by techniques, such as FTIR, 1HNMR, TGA, XRD, and elemental analysis.

Results:
The average molar weight and the intrinsic viscosity of alginate from Sargassum bovaneum
with a yield of 24.38% were 41.53 kDa and 0.9 dL/g 105 cps (2.5% C). Results showed that as the alginate
concentration increased, the flow rate decreased with a mild slope.

Conclusion:
Elemental analysis (CHNS) of alginate derivatives indicated the percent of amine groups
to be increased after amination reaction in comparison to corresponding alginates. The thermal analysis
results indicate that the thermal stability of the sodium alginate is better than graft copolymers. Synthetic
derivatives showed no particular sensitivity to environmental stimuli, such as temperature and
pH changes.