bluuebunny/crossref_metadata_2025_split huggingface dataset:
DPP6 and MFAP5 are associated with immune infiltration as diagnostic biomarkers in distinguishing uterine leiomyosarcoma from leiomyoma
Authors: Ke Yumin , You LiuXia , Xu YanJuan , Wu Dandan , Lin Qiuya , Wu Zhuna 
Published: November 2022

ObjectiveUterine leiomyosarcoma (ULMS) is the most common subtype of uterine sarcoma and is difficult to discern from uterine leiomyoma (ULM) preoperatively. The aim of the study was to determine the potential and significance of immune-related diagnostic biomarkers in distinguishing ULMS from ULM.MethodsTwo public gene expression profiles (GSE36610 and GSE64763) from the GEO datasets containing ULMS and ULM samples were downloaded. Differentially expressed genes (DEGs) were selected and determined among 37 ULMS and 25 ULM control samples. The DEGs were used for Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Disease Ontology (DO) enrichment analyses as well as gene set enrichment analysis (GSEA). The candidate biomarkers were identified by least absolute shrinkage and selection operator (LASSO) and support vector machine recursive feature elimination (SVM-RFE) analyses. The receiver operating characteristic curve (ROC) was applied to evaluate diagnostic ability. For further confirmation, the biomarker expression levels and diagnostic value in ULMS were verified in the GSE9511 and GSE68295 datasets (12 ULMS and 10 ULM), and validated by immunohistochemistry (IHC). The CIBERSORT algorithm was used to calculate the compositional patterns of 22 types of immune cells in ULMS.ResultIn total, 55 DEGs were recognized via GO analysis, and KEGG analyses revealed that the DEGs were enriched in nuclear division, and cell cycle. The recognized DEGs were primarily implicated in non−small cell lung carcinoma and breast carcinoma. Gene sets related to the cell cycle and DNA replication were activated in ULMS. DPP6 and MFAP5 were distinguished as diagnostic biomarkers of ULMS (AUC = 0.957, AUC = 0.899, respectively), and they were verified in the GSE9511 and GSE68295 datasets (AUC = 0.983, AUC = 0.942, respectively). The low expression of DPP6 and MFAP5 were associated with ULMS. In addition, the analysis of the immune microenvironment indicated that resting mast cells were positively correlated with DPP6 and MFAP5 expression and that eosinophils and M0 macrophages were negatively correlated with DPP6 expression (P&lt;0.05).ConclusionThese findings indicated that DPP6 and MFAP5 are diagnostic biomarkers of ULMS, thereby offering a novel perspective for future studies on the occurrence, function and molecular mechanisms of ULMS.
Predicting resolution of hypoglycemia with and without dextrose infusion in newborn infant of gestational diabetic mothers
Authors: Ibrahim Mohammed , Hou Wei , Decristofaro Joseph , Maduekwe Echezona T. 
Published: November 2022

IntroductionNeonatal hypoglycemia (NH) may lead to significant neurological impairment if left untreated. Infants of gestational diabetic mothers (IGDM) are at increased risk of early NH and need to be screened. However, it is challenging to predict management with or without intravenous dextrose once hypoglycemia is identified. We evaluated the association between hypoglycemia risk scores at 1-hour of life and the need for intravenous dextrose for hypoglycemia resolution in IGDM.MethodsThis was a retrospective cohort study of IGDM born at a gestational age ≥35 weeks from January 2015 to December 2017. NH was the disease of interest. The outcomes were the association of hypoglycemia risk score (HRS) with and without intravenous dextrose for hypoglycemia resolution. Each infant's hypoglycemia risk score (HRS) was calculated using data extracted from the maternal and neonatal electronic medical records. Resolution of hypoglycemia with and without intravenous dextrose was compared between the low HRS (0–1) group and the high HRS (2–5) group.ResultsSixty-five infants were included in the study with a mean gestational age of 38.2 ± 1 weeks for low HRS and 38.0 ± 2 weeks for high HRS. While more children with high HRS were delivered by cesarean section (p = 0.04), hypoglycemia resolved more frequently without intravenous dextrose in infants with low HRS (p = 0.03).ConclusionIGDM is at increased risk of NH. The resolution of hypoglycemia without dextrose infusion is frequently associated with low HRS at 1-hour of life. Early identification using HRS of IGDM whose hypoglycemia will resolve with or without intravenous dextrose may help clinicians triage newborns to either stay in the nursery or transfer for more invasive care.
Optimizing antimicrobial therapy in urinary tract infections: A focus on urine culture and sensitivity testing
Authors: Alkhawaldeh Rama , Abu Farha Rana , Abu Hammour Khawla , Alefishat Eman 
Published: November 2022

Objectives: This cross-sectional study was conducted at Jordan university hospital to evaluate the impact of microbial culture data and sensitivity results on optimizing UTI treatment.Methods: All positive urine cultures requested for adult patients (≥18 years) admitted to Jordan University Hospital (JUH) within the period from January 2019–July 2021 were evaluated. The antibiotics prescribed before and after culture data and sensitivity results were compared to evaluate the impact of these diagnostic measures on optimizing UTI treatment.Results: During the study period, 2400 urine cultures revealed positive results. Among those patients, 1,600 (66.7%) were discharged before the availability of culture results and excluded. Of the remaining 800 patients, 701 patients (87.6%) received empiric treatment. After culture and sensitivity results were available, overall, 84 (10.5%) patients had optimization (improvement) in their UTI management after culture results were known, while 6 (0.8%) patients had a worsening in their treatments. Based on the culture results, we found that only 12.4% of patients were appropriately treated before and after the culture results. Moreover, our results revealed that 31.9% were inappropriately treated for their UTIs before and after culture results.Conclusion: This study revealed an alarmingly high rate of inappropriate treatment of UTIs despite the availability of urine culture and sensitivity data, and that culture results were not used to optimize treatment strategies for UTI. This practice can potentially result in poor health-related outcomes and adversely affects efforts to battle AMR. Multifaceted strategies must be implemented to help clinicians follow the best current evidence and current guidelines in their selection of antibiotics for the management of UTIs.
Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
Authors: Škovranová Gabriela , Čulenová Marie , Treml Jakub , Dzurická Lucia , Marova Ivana , Sychrová Alice 
Published: November 2022

Antimicrobial resistance is a public health threat and the increasing number of multidrug-resistant bacteria is a major concern worldwide. Common antibiotics are becoming ineffective for skin infections and wounds, making the search for new therapeutic options increasingly urgent. The present study aimed to investigate the antibacterial potential of prenylated phenolics in wound healing. Phenolic compounds isolated from the root bark of Morus alba L. were investigated for their antistaphylococcal potential both alone and in combination with commonly used antibiotics. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by microdilution and agar method. Synergy was investigated using the checkerboard titration technique. Membrane-disrupting activity and efflux pump inhibition were evaluated to describe the potentiating effect. Prenylated phenolics inhibited bacterial growth of methicillin-resistant Staphylococcus aureus (MRSA) at lower concentrations (MIC 2–8 μg/ml) than commonly used antibiotics. The combination of active phenolics with kanamycin, oxacillin, and ciprofloxacin resulted in a decrease in the MIC of the antimicrobial agent. Kuwanon C, E, T, morusin, and albafuran C showed synergy (FICi 0.375–0.5) with oxacillin and/or kanamycin. Prenylated phenolics disrupted membrane permeability statistically significantly (from 28 ± 16.48% up to 73 ± 2.83%), and membrane disruption contributes to the complex antibacterial activity against MRSA. In addition, kuwanon C could be considered an efflux pump inhibitor. Despite the antibacterial effect on MRSA and the multiple biological activities, the prenylated phenolics at microbially significant concentrations have a minor effect on human keratinocyte (HaCaT) viability. In conclusion, prenylated phenolics in combination with commonly used antibiotics are promising candidates for the treatment of MRSA infections and wound healing, although further studies are needed.
Effect of rejection sensitivity on the development of anxious-depressive attack in Japanese outpatients: The mediating roles of rumination, social anxiety, and depressive symptoms
Authors: Noda Shota , Masaki Mina , Kishimoto Tomokazu , Kaiya Hisanobu 
Published: November 2022

ObjectiveAnxious-depressive attack (ADA) is a cluster of symptoms, including sudden and intense anxiety or depression, intrusive rumination about negative memories or future worries, prominent agitation, impatient behavior, and/or loneliness; in some cases, symptoms include a wide range of violent coping behaviors to manage emotional distress. Four characteristics—rejection sensitivity, rumination, social anxiety symptoms, and depressive symptoms—are thought to be associated with the development of ADA. However, the complex relationships among these factors have not been clarified. In this study, we aimed to examine the mechanism by which these four characteristics influence the development of ADA.MethodsWe conducted a structured interview about ADA with 332 outpatients, who completed several self-report measures, to assess rejection sensitivity, rumination, social anxiety symptoms, and depressive symptoms.ResultsA structural equation model showed goodness-of-fit with the data. These findings suggest that rejection sensitivity may demonstrate a direct effect on the occurrence of ADA. Furthermore, rejection sensitivity might affect depressive symptoms through rumination and social anxiety symptoms and consequently contribute to the development of ADA.ConclusionThese results provide preliminary evidence that rejection sensitivity contributes to the development of ADA.
The Frontal Assessment Battery (FAB) effectively discriminates between MCI and dementia within the clinical spectrum of neurochemically confirmed Alzheimer’s disease
Authors: Aiello Edoardo Nicolò , Verde Federico , Milone Ilaria , Giacopuzzi Grigoli Eleonora , Dubini Antonella , Carelli Laura , Ferrucci Roberta , Priori Alberto , Ratti Antonia , Torresani Erminio , Ticozzi Nicola , Silani Vincenzo , Poletti Barbara 
Published: November 2022

BackgroundThis study aimed at testing the ability of the frontal assessment battery (FAB) to differentiate between patients with mild cognitive impairment (MCI) and dementia due to Alzheimer’s disease (AD), as well as comparing its discriminative power to that of the Mini-Mental State Examination (MMSE).MethodsThe present retrospective cohort includedN= 107 Aβ-positive patients diagnosed with either MCI due to AD (N= 40) or probable AD dementia (ADD;N= 67). A two-step multiple logistic regression (MLR) was run to predict an MCI vs. ADD diagnosis based on FAB scores. Within the baseline step, demographics, disease duration, MMSE scores, and information on cognitive phenotypes were entered, with the FAB being added within the second step. Receiver-operating characteristics analyses were also run to derive intrinsic and post-test diagnostics.ResultsWithin the baseline MLR step, only lower MMSE scores predicted the occurrence of ADD; by adding the FAB, which likewise was able to discriminate between MCI and ADD (p= 0.016), a significant increase in model fit was detected (p= 0.007). The diagnostic efficiency of the FAB (AUC = 0.85) was comparable (p= 0.583) to that of the MMSE (AUC = 0.82), also yielding good intrinsic and post-test diagnostics, which were comparable to those of the MMSE.DiscussionThe FAB is a diagnostically sound screener to discriminate between MCI and ADD, independently of patients’ overall cognitive profile. In doing so, the FAB is comparable to the MMSE, and the complementation of the latter with the former is advisable in order to increase the accuracy in differentiating between MCI and ADD within screening sessions.
Promotion strategy for online healthcare platform during the COVID-19 pandemic: Evidence from Spring Rain Doctor in China
Authors: Zhang Lanting , Lv Dan , Li Weijia , Xing Zeyu 
Published: November 2022

IntroductionOnline healthcare platform (OHP) is a new form of medical treatment that solves the problems of an unbalanced distribution of medical resources in China. Especially during the COVID-19 pandemic, OHP has greatly reduced the medical pressure of the hospital and the risk of cross-infection.MethodsBased on self-determination theory (SDT) (Ryan and Deci, 2000), privacy calculus theory (PCT) (Culnan, 1999) and perceived value theory (PVT) (Choi, 2004), this study uses evolutionary game theory to analyze behavioral strategies and their dynamic evolution in the promotion of OHP. Moreover, we conduct numerical simulations with the help of program compilation.ResultsThe results demonstrate that (1) both the qualification inspection of doctors and the investment in information protection influence doctors’ participation in and patients’ usage of OHP; (2) both the initial probabilities of doctor participation and patient usage influence the multi-game results; (3) the trend of doctors joining OHP is affected by registration cost, time cost, and reputation loss; and (4) the trend of patients using online healthcare is mainly decided by the cost.ConclusionThis study takes the Spring Rain Doctor as an example to verify the game results. To further popularize online medical treatment among patients, the platform should attach importance to the inspection of doctors and the protection of privacy information and strengthen its publicity in remote places.
A specific cognitive behavioral group therapy program for stimulant use disorder
Authors: Karsinti Emily , Vorspan Florence , Therribout Norman , Icick Romain , Bloch Vanessa , Fortias Maeva , Piani Kristel , Romo Lucia 
Published: November 2022

IntroductionStimulant use is an important health issue. In the US in 2018, 2.8% of males and 1.5% of females older than 18 had used cocaine in the preceding 12 months.ObjectiveTo intervene in a specific targeted group of Stimulant Use Disorder (SUD) patients according to CBT and relapse prevention theories, and to determine the program’s feasibility and attendance.MethodStimulant Use Disorder patients in addiction care were evaluated for addictive, psychological and psychiatric dimensions at baseline and conclusion in a 9-session CBT group program with several themes: define SUD, enhance motivation, involve close companions, cope with craving, decline a proposal, solve problems, invite expert patients, invest time and money, and review content.ResultsIn total, 41 patients attended at least one session. They were mainly poly dependent, primarily cocaine users. Sixty percent of the population also suffered from another psychiatric comorbidity. Median attendance for participants was 7/9 sessions.ConclusionA specific targeted CBT group for stimulant dependent highly comorbid patients is feasible. These findings suggest that peers should be included in addiction care services.
COVID-19 hospitalizations and patients' age at admission: The neglected importance of data variability for containment policies
Authors: Azzolina Danila , Comoretto Rosanna , Lanera Corrado , Berchialla Paola , Baldi Ileana , Gregori Dario 
Published: November 2022

IntroductionAn excess in the daily fluctuation of COVID-19 in hospital admissions could cause uncertainty and delays in the implementation of care interventions. This study aims to characterize a possible source of extravariability in the number of hospitalizations for COVID-19 by considering age at admission as a potential explanatory factor. Age at hospitalization provides a clear idea of the epidemiological impact of the disease, as the elderly population is more at risk of severe COVID-19 outcomes. Administrative data for the Veneto region, Northern Italy from February 1, 2020, to November 20, 2021, were considered.MethodsAn inferential approach based on quasi-likelihood estimates through the generalized estimation equation (GEE) Poisson link function was used to quantify the overdispersion. The daily variation in the number of hospitalizations in the Veneto region that lagged at 3, 7, 10, and 15 days was associated with the number of news items retrieved from Global Database of Events, Language, and Tone (GDELT) regarding containment interventions to determine whether the magnitude of the past variation in daily hospitalizations could impact the number of preventive policies.ResultsThis study demonstrated a significant increase in the pattern of hospitalizations for COVID-19 in Veneto beginning in December 2020. Age at admission affected the excess variability in the number of admissions. This effect increased as age increased. Specifically, the dispersion was significantly lower in people under 30 years of age. From an epidemiological point of view, controlling the overdispersion of hospitalizations and the variables characterizing this phenomenon is crucial. In this context, the policies should prevent the spread of the virus in particular in the elderly, as the uncontrolled diffusion in this age group would result in an extra variability in daily hospitalizations.DiscussionThis study demonstrated that the overdispersion, together with the increase in hospitalizations, results in a lagged inflation of the containment policies. However, all these interventions represent strategies designed to contain a mechanism that has already been triggered. Further efforts should be directed toward preventive policies aimed at protecting the most fragile subjects, such as the elderly. Therefore, it is essential to implement containment strategies before the occurrence of potentially out-of-control situations, resulting in congestion in hospitals and health services.
Optimizing national border reopening policies in the COVID-19 pandemic: A modeling study
Authors: Huang Jiaoling , Qian Ying , Shen Wuzhi , Chen Yong , Zhao Laijun , Cao Siqi , Rich Eliot , Pastor Ansah John , Wu Fan 
Published: November 2022

ObjectiveAfter emergence of the COVID-19 pandemic and subsequent restrictions, countries worldwide have sought to reopen as quickly as possible. However, reopening involves the risk of epidemic rebound. In this study, we investigated the effective policy combination to ensure safe reopen.MethodsOn the basis of the classical SEIR epidemic model, we constructed a COVID-19 system dynamics model, incorporating vaccination, border screening, and fever clinic unit monitoring policies. The case of China was used to validate the model and then to test policy combinations for safe reopening.FindingsVaccination was found to be crucial for safe reopening. When the vaccination rate reached 60%, the daily number of newly confirmed COVID-19 cases began to drop significantly and stabilized around 1,400 [1/1,000,000]. The border screening policy alone only delayed epidemic spread for 8 days but did not reduce the number of infections. Fever clinic unit monitoring alone could reduce the peak of new confirmed cases by 44% when the case identification rate rose from 20 to 80%. When combining polices, once the vaccination rate reached 70%, daily new confirmed cases stabilized at 90 [0.64/1,000,000] with an 80% case identification rate at fever clinic units and border screening. For new variants, newly confirmed cases did not stabilize until the vaccination rate reached 90%.ConclusionHigh vaccination rate is the base for reopening. Vaccination passport is less effective compared with a strong primary care monitoring system for early detection and isolation of the infected cases.
In Transition towards Organic Farming: Effects of Rock Phosphate, Coral Lime, and Green Manure on Soil Fertility of an Acid Oxisol and the Growth of Soybean (Glycine max L. Merr.) Seedlings
Authors: Huang Robert , Hue Nguyen 
Published: November 2022

Maintaining soil fertility and obtaining good crop yields in highly weathered tropical soils through organic practices–without chemical/synthetic inputs—requires a scientific approach and skillful managements, especially for phosphorus (P) nutrient. Our objective was to find a combination of lime and rock phosphate additions that made soil pH low enough so that rock phosphate would be adequately soluble, yet high enough so soil acidity is not harmful to most crops. Thus, a controlled (greenhouse) experiment was conducted to quantify soil properties, and soybean (Glycine max cv. Kahala) growth when rock phosphate, coral lime, and cowpea (Vigna unguiculata) green manure were applied as organic amendments to an acid, nutrient poor Oxisol of Hawaii. The treatments were a factorial combination of 3 application rates (0, 1, 2 g/kg) of coral lime (86% CaCO3 equivalent) from Western Samoa, 3 rates (0, 75, 150 mg/kg total P) of rock phosphate (10.6% total P, and 3.7% citrate extractable P) from central Florida, and 3 rates (0, 5, 10 g/kg) of a local cowpea green manure (2.7% N, 2.8% K). Each treatment was replicated 3 times, yielding a total of 81 pots of 2 kg soil each. Soybean seedlings were grown as a test crop. Our results showed that a combination of 1 g/kg (2 tons/ha) of lime and 75 mg/kg (150 kg P/ha) of rock phosphate provided enough P for soybean growth and simultaneously alleviated soil acidity problems (the green manure was to supply adequate N and K to the crop). Corresponding soil parameters were: soil pH = 5.2, exchangeable (KCl-extractable) Al = 3.6 mg/kg, Olsen (NaHCO3- extractable) P = 11 mg/kg, and soil-solution P of 0.05 mg/L. Our results lent support to the sustainable potential of organic farming.
Comparative Analysis of Laparoscopic and Robotic Transperitoneal Adrenalectomy Performed at a Single Institution
Authors: Choi Yun Suk , Lee Ji Sun , Yi Jin Wook 
Published: November 2022

Background and Objectives: Laparoscopic adrenalectomy (LA) is the standard surgical approach for adrenalectomy. At present, robotic adrenalectomy (RA) has been introduced at various hospitals. This study evaluated our initial experience with robotic adrenalectomy compared with conventional laparoscopic adrenalectomy. Materials and Methods: From October 2018 to March 2022, 56 adrenalectomies were performed by a single endocrine surgeon. Thirty-two patients underwent LA (LA group), and twenty-four patients underwent RA (RA group). Results: Patients in the RA group were significantly younger than those in the LA group (48.6 ± 9.7 years vs. 55.1 ± 11.4 years, p = 0.013). The RA group had a shorter operation time than the LA group (76.1 ± 28.2 min vs. 118.0 ± 54.3 min, p < 0.001). The length of hospital stay and postoperative pain level between the two groups were similar. There were no complications in the RA group. There was no significant difference in the pathologic diagnosis between the two groups. The cost of surgery was significantly higher in the RA group than in the LA group (5288.5 US dollars vs. 441.5 ± 136.8 US dollars, p < 0.001). Conclusions: In our initial experience, RA showed a shorter operation time than LA and no complications. RA could be a viable alternative surgical option for adrenalectomy, notwithstanding its higher cost.