bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fungal community diversity profiles in pit mud samples from Chinese Strong-flavour liquor pit
Authors: Pu Shunchang , Shi CuiE , Guang JiaQuan , Shoubao Yan 
Published: May 2022

Abstract
Pit mud is a specialized type of fermented soil that is essential for the distillation of Chinese strong-flavour liquor. In this analysis, differences in fungal community structures and physicochemical properties in pit mud samples from different spatial positions within fermentation cellars were analyzed, revealing unique characteristic multidimensional pit mud fungal community profiles. Penicillium fuscoglaucum, Penicillium glandicola, Aotearoamyces nothofagi, Malassezia restricta, Penicillium lanosocoeruleum, Penicillium crustosum, and Aspergillus tonophilus were detected only in pit mud samples from the upper cellar wall, while Alternaria alstroemeriae, Trichosporon insectorum, Fusarium equiseti, Calonectria pseudoreteaudii, Penicillium clavigerum, Penicillium compactum, Ascochyta phacae, Metarhizium frigidum, Alternaria burnsii, Fusarium nurragi, and Didymella keratinophila were present only in the middle cellar wall layer. Additionally, Alternaria zantedeschiae and Ilyonectria cyclaminicola were only present in pit mud samples from the lower cellar wall, while Leptobacillium leptobactrum, Calonectria queenslandica, Aspergillus appendiculatus, and Candida pseudolambica were only detected in samples of pit mud from the cellar bottom. Moisture, pH, PO43-, acetic acid, humus, K+, Mg2+, Ca2+, acetic acid, butyric acid, and caproic acid levels in these different pit mud positions exhibited a rising incremental pattern from the upper wall layer to the bottom layer, whereas lactic acid levels were significantly lower in the bottom pit mud layer relative to these other layers. Moisture, pH, and NH4+-N were identified as the three most significant factors associated with fungal community composition through a redundancy analysis. Overall, these findings may offer a theoretical foundation for future efforts to improve or standardize artificial pit mud.
Transit Dosimetry of Stereotactic Body Radiotherapy Treatments with Electronic Portal Dosimetry Device in Patient with Spinal Implant
Authors: Yedekci Yağız , Elmalı Aysenur , demirkıran Gökhan , Özyigit Gökhan , Yazıcı Gözde 
Published: May 2022

Abstract
Aim
In recent years, the use of the Electronic Portal Imaging Device (EPID) as an in vivo dosimeter has become widespread. However, reports of EPID for stereotactic body radiotherapy (SBRT) applications is scarce. There is no data on this topic especially when there are high-density materials in the radiation field. In this study, we aimed to investigate the dose distributions of SBRT treatment plans in patients with spinal implants by in vivo EPID dosimetry.
Material Methods:
Implants were inserted in phantoms that mimic the vertebrae, and VMAT plans were created on the phantoms to deliver 16 Gy radiation doses to the target in 1 fraction. In vivo EPID measurements were performed for each irradiation. The results were compared with the treatment planning system using the gamma analysis method.
Results
According to the gamma analysis results, while the non-implant model met the acceptance criteria with a rate of 95.4%, the implanted models did not pass the test with results between the rates of 70–73%. In addition, while the dose difference in the isocenter was 1.3% for the non-implanted model, this difference was observed to be between 7% and 8% in the implanted models.
Conclusion
Our study revealed that EPID can be used as in vivo dosimetry for the VMAT-SBRT applications. However, unacceptable dose differences were obtained by in vivo EPID dosimetry in the VMAT-SBRT applications of patients with an implant. In the treatment of such patients, alternative treatment methods should be preferred in which the interaction of the implants with radiation can be prevented.
Effect of anemoside B4 on milk whey in clinical mastitis-affected cows elucidated using tandem mass tag (TMT)-based quantitative proteomics
Authors: Shen Liu-hong , Zhang Yue , Shen Yu , Su Zhe-tong , Yu Shu-min , Cao Sui-zhong , Zong Xiao-lan 
Published: May 2022

Abstract
Objective
Intramuscular injection of anemoside B4 (AB4) has a superior therapeutic effect on clinical mastitis in lactating cows. Here, we explored AB4’s effect on milk whey in clinical mastitis-affected cows using proteomics.
Methods
Among fifty clinical mastitis cows received AB4 administration (0.05ml/kg/day, for seven days), twelve healed cows were selected and marked as group T. Twelve clinically heathy cows received the same dose of saline for seven days, marked as group C. Collected milk whey of group T before and after AB4 administration marked as T1 and T2, respectively. And the milk whey of group C after saline injection marked as C1. Milk whey protein changes were detected with tandem mass tag-based quantitative proteomic.
Results
We identified 872 quantifiable proteins in the samples. Among them, 511 proteins between T1 and C1, and 361 proteins between T2 and T1 were significantly altered. T1 than C1 had significantly more proteins associated with inflammatory damage and trans-endothelial migration of leukocytes, whereas these proteins were reduced in T2 treated with AB4. Compared with C, proteins associated with fibrin clot degradation and complement system activation were downregulated in T1 but upregulated in T2.
Conclusion
In summary, AB4 can exert its therapeutic effect on clinical mastitis in cows mainly by reducing inflammatory damage, activating the complement system, inhibiting trans-endothelial migration of leukocytes, and promoting degradation of milk fibrin clots.
Influence of tinnitus annoyance on hearing-related quality of life in cochlear implant recipients
Authors: Assouly Kelly K.S. , Arts Remo A.G.J. , Graham Petra L. , Dijk Bas , James Chris J. 
Published: May 2022

Abstract
Among cochlear implant (CI) recipients, there is no consensus on the impact of tinnitus on hearing-related quality of life (QoL). The aim of this study was to assess the relationship between hearing-related QoL measured by the Speech Spatial and Qualities of Hearing questionnaire (SSQ12) and tinnitus annoyance or perceived change in tinnitus annoyance after cochlear implantation. The study sample consisted of 2322 implanted adults across France, Germany, Ireland, Italy, the Netherlands, Sweden and the United Kingdom. Information relating to QoL, measured using the SSQ12, tinnitus annoyance and change in tinnitus annoyance, assessed using single-item questions, were collected one or more years post-implantation. The relationship between SSQ12 total score and tinnitus annoyance or change in tinnitus annoyance was analysed using linear models adjusted for age and unilateral versus bilateral implants. Tukey pairwise tests were used to compare tinnitus levels. Tinnitus prevalence was 33.9% post-implantation. This prevalence varied significantly with age. Recipients with tinnitus had a significantly lower SSQ12 score than recipients without tinnitus. The SSQ score varied significantly with tinnitus annoyance, age and unilateral versus bilateral implants. Overall, cochlear implant recipients who experienced less bothersome tinnitus showed better subjective hearing-related QoL. Healthcare professionals should be aware of the need to prioritise tinnitus management for CI recipients to optimize the benefit of CI.
MiRNA-6870-3p regulates LPS-induced epicardial adipose tissue inflammatory genes via targeting Tollip-mediated JNK and NF-κB signaling in coronary artery disease
Authors: Wu JianGuo , Yang Wen-kai , Huang Hao-Zong , Tang Kai , Zheng Guang-Di 
Published: May 2022

Abstract
Objective
MiR-6870-3p acts as a crucial regulator of gene expression at the posttranscriptional level and participates in immune responses. However, the roles of miR-6870-3p and its target genes and their underlying mechanisms in the inflammatory responses of epicardial adipose tissues (EATs) are unknown.
Methods
MiRNA microarray was used to collect miRNA expression profiles in EATs from five patients with coronary artery disease (CAD) and four individuals without CAD (n-CAD). Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to check the expression of miR-6870-3p in CAD and n-CAD EATs. The mRNA and protein expression levels of Tollip and the key genes of the TLR4 signaling pathway were examined by qRT-PCR and Western blot. The levels of inflammatory factors in the cell supernatant were measured by enzyme-linked immunosorbent assay. Bioinformatics algorithms were used to predict the target gene of miR-6870-3p, and the result was validated by dual-luciferase reporter assay. The protein expression levels of JNK and NF-κB were measured by Western blot.
Resules:
Our results showed that miR-6870-3p was remarkably higher in CAD EATs than in n-CAD EATs. MiR-6870-3p was positively correlated with TLR4, IL-6, JNK, NF-κB (p65), and TNF-α in CAD EAT samples. Lipopolysaccharide (LPS) treatment upregulated miR-6870-3p mRNA expression and downregulated Tollip mRNA and protein expression in macrophages. MiR-6870-3p upregulation also aggravated the production of proinflammatory cytokines, such as TNF-α and IL-6, when the macrophages were stimulated with LPS. The result of luciferase reporter assays confirmed that miR-6870-3p directly targets Tollip. Moreover, miR-6870-3p upregulation in macrophages resulted in the activation of the JNK/NF-κB pathway.
Conclusion
Our study concluded that miR-6870-3p regulates human EAT inflammation by targeting Tollip-mediated JNK and NF-κB signaling events.
Cervical capillary intra-extramedullary hemangioblastoma. An unusual tumor in an unusual site
Authors: Colamaria Antonio , Blagia Maria , Carbone Francesco , Fochi Nicola Pio , Leone Augusto 
Published: May 2022

Abstract
Spinal hemangioblastoma is an infrequent, highly vascularized tumor which can cause compression, extensive bleeding or syringomyelia. We present the case of a 32-year-old woman presenting with persisting headaches, whose MRI disclosed an intra-extradural mass extending from the obex to C2. A brief description of the treatment is provided. A surgical fenestration of the associated syrinx should not be taken into account, as it seems to spontaneously regress after the removal of the primary lesion.
Which euploid single embryo transfer strategy is preferable in advanced maternal aged; Fresh versus frozen
Authors: ÖZDEMİR FATMA , Oner Gokalp , Kahraman Semra , Sahin Yucel , Yelke Hakan 
Published: May 2022

Abstract
Purpose: Herein, we aimed to compare two commonly used single embryo transfer (ET) strategies, fresh and frozen-thawed euploid blastocyst transfer protocols via trophectoderm biopsy after preimplantation genetic screening (PGS) in advanced maternal aged (AMA).Study Design: This study was designed as a single-center cohort study.A total of 317 patients aged older than 37 years old who underwent single fresh or frozen-thawed day five or day 6 ET with trophectoderm biopsy examined retrospectively. PGS was performed on the embryos of 138 patients, and fresh transfers were preferred, while embryos of 179 patients were vitrified, and then frozen-thawed euploid single ET was chosen after PGS. Percentage of the euploid embryo, implantation rates, pregnancy rates, pregnancy continuity rates, clinical and biochemical abortion rates were compared.Results: Age, body mass index, infertility duration,previous ART cycles,days of stimulation, andAnti-Mullerian hormone levels did not reveal statistically significant difference between the groups. The retrieved oocyte, MII oocytes, and fertilized ovum (2PN) numbers were higher in the Frozen-thawed group. The percentage of a euploid embryo (32% vs. 34.8%) was similar between the groups. Implantation rates (46% vs. 62%), pregnancy rates (49% vs. 66%), ongoing pregnancy rates (36.7% vs. 52.6%), and live birth percentage (35.4% vs. 51.6%) were significantly higher in the frozen-thawed embryo transfer group, while the clinical and biochemical abortion rates did not reveal significant difference. Conclusion: Frozen-thawed single euploid ET provides sufficient time for comprehensive genetic evaluations and increases the rates of implantation and pregnancy rates compared to the fresh single euploid ET in AMA patients with PGS.
Identification of biomarkers and therapeutic targets in the peripheral immune landscape from coronary artery disease
Authors: Feng Xiaoteng , Zhang Yifan , Du Min , Li Sijin , Ding Jie , Wang Jiarou , Wang Yiru , Liu Ping 
Published: May 2022

Abstract
Background: Although it plays a vital role in the whole process of various cardiovascular diseases, the characteristic immune changes of peripheral blood in patients with CAD have not been well recognized. This study aimed to determine the expression of differentially expressed immune-related genes (DE-IRGs) in CAD by screening three gene chips downloaded from the Gene Expression Omnibus (GEO) database. Methods: We downloaded CAD and Healthy groups’ information and IRGs from the GEO database and Immport database (ImmpDb) respectively. By R software, we processed GO and KEGG analysis to find out the function and main enriched pathway between two groups. LASSO method was used to identify genes for diagnostic testing. CIBERSORT analysis revealed the differential immune cell distribution. Finally, we verified the expression of these differential genes through an animal project.Results: 762 differentially expressed genes (DEGs) were identified in this process, which mainly enriched in infection, immune response and neural activity and might participate in the CAD process by affecting the extracellular matrix formation and receptor-ligand activity functions. In total, 58 DE-IRGs were subsequently obtained by overlapping the DEGs and immune-related genes downloaded from ImmpDb. Through LASSO regression, CCR9, CER1, CSF2, IL13RA1, INSL5, MBL2, MMP9, MSR1, NTS, TNFRSF19, CXCL2, HTR3C, IL1A, and NR4A2 among the 58 DE-IRGs were distinguished as peripheral biomarkers of CAD, which showed eligible diagnostic capabilities separately. These screened genes were then validated by an animal subject. The real-time PCR test showed that CCR9, CSF2, IL13RA1, and NTS had significant differences between the CAD model and healthy control samples, suggesting that they were promising diagnostic and therapeutic targets. Besides, CIBERSORT algorithm analysis showed that the immune-related cells existed characteristic distribution in CAD, which was discovered to have a close relationship with some specific DE-IRGs. Conclusions: In summary, we confirmed four peripheral immune biomarkers related to CAD and provided new potential targets for regulating the immune relevant mechanism, laying a foundation for further excavation of CAD immune mechanism.
Agricultural practices regulate the seasonality of groundwater-river nitrogen exchanges
Authors: Pinardi Monica , Soana Elisa , Severini Edoardo , Racchetti Erica , Celico Fulvio , Bartoli Marco 
Published: May 2022

Abstract
Soil system budgets are generally performed aggregating annual nutrient inputs and outputs over arable land to infer their use efficiency and water pollution risk in watersheds with intensive agriculture and animal farming. They are seldom partitioned into seasonal budgets and matched with seasonal transport of nutrients in adjacent river reaches. In this work we calculated seasonal soil nitrogen (N) budgets in a Mincio River sub-basin (Northern Italy), and we analyzed the dissolved inorganic N net export in the river reach draining such sub-basin. Our results suggest pronounced seasonal differences of soil system budgets with N excess in winter and even more in spring, equilibrium among sources and sinks during autumn and N deficit during summer. Seasonal inorganic N loads transported by the river were not correlated with soil system budgets as they peaked in late summer and were at their minimum in early spring. Fertilization uncoupled to significant uptake supports N excess in winter and spring, whereas crop uptake uncoupled to N inputs supports summer N deficit. Within the river reach potential nitrification cannot explain nitrate accumulation in the water, suggesting alternative dynamics, related to the seasonality of irrigation practices, driving the local hydrology. Flood irrigation results in large soil nitrate solubilization, horizontal and vertical transport and in upward vertical migration of the groundwater piezometric head during the spring and the summer. River water is likely replaced by nitrate-rich groundwater when the groundwater recharge exceeds a certain threshold coinciding with late summer. Irrigation is then interrupted and the piezometric head, together with nitrate exchange, decreases. This work suggests that a deep understanding of N dynamics in watersheds with intensive agriculture and animal farming and irrigation via flooding on permeable soils needs the implementation of hydrological studies and the reconstruction of the vertical pathways of nitrate and of river-groundwater interactions. Moreover, the partitioning of annual into seasonal N budgets and their combination with irrigation practices allows the identification of hot moments in N cycling. Agricultural practices minimizing nitrate excess, its mobility and the risk of ground and surface water pollution are suggested for this area.
Adherence to antiretroviral therapy among adolescents living with HIV in Jamaica
Authors: Dyer R , Harrison A , Lewis-O’Connor K , Pierre R B 
Published: May 2022

Abstract
Objective:To characterize adherence patterns to antiretroviral therapy (ART) and identify factors affecting optimal adherence among adolescents living with HIV (ALHIV) in Kingston, Jamaica during the Covid-19 pandemic. Design and Methods:During August-October 2021, we conducted a cross-sectional study on adherence (no missed doses in preceding four days) among adolescents 10-18 years attending three outpatient departments in the Kingston Metropolitan Area. Adherence factors were evaluated using a self-administered, interviewer-assisted structured questionnaire. Biochemical and clinicopathological information were retrieved from patients’ medical records. Multivariate logistic regression was used to determine likelihood of adherence for given adherence factors. Results: Of 65 participating clients, 92.3 % were perinatally infected, mean (SD) age 15.4 (2.0) years and 61.5% were female. Overall self-reported adherence was 66.1% (43/65) and higher among adolescents in residential care versus family care (p=0.002). Median (IQR) viral load 19 copies/ml/103 (IQR 19-51) was lower (p=0.010) and median (IQR) CD4+ count 701 cells per µL (IQR 501-1052) higher (p= 0.016) among adolescents in residential care compared to family care. Adherence was 4 times more likely among adolescents at UHWI (OR=4.53, 95% CI (1.25, 16.43), knowledgeable about ARVs (OR= 4.31, (1.09,17.04), and with reduced appointments due to Covid-19 pandemic (OR= 5.36, (1.09, 26.41) and 4 times less likely if cared for by relatives, experiencing side effects, higher pill burden or hospitalized with complications.Conclusions:Medication, caregiver-related and health system management factors are both enablers and barriers of adherence for ALHIV, and the importance of caregivers’ support, treatment literacy and simplified treatment regimens are highlighted.
Green credits, Green securities, and Environmental Quality: A comparative Analysis of Sustainable development across Chinese Provinces.
Authors: Kassi Diby Francois , Li Yao , Gnahe Franck Edouard , Shaikh Ruqia , Tuo Siele Jean 
Published: May 2022

Abstract
This paper analyzes the effects of green credits and green securities on environmental quality in a multivariate EKC framework across Chinese provinces during the 1992Q1-2020Q4 period. In this comparative analysis, we employ the DOLS and FMOLS estimators along with the method of moments-quantile regression (MM-QR) with the fixed-effects model. First, the FMOLS and DOLS methods show that short-term green credits (STLs) and green securities (CAPs) reduce carbon emissions (CE) in Chinese provinces. Unlike the Western region, long-term green credits (LTLs) improve environmental quality in the Eastern and Central regions. Second, large STLs and CAPs affect CE more than their low levels, unlike large LTLs. Third, STLs and CAPs increase CE only in the most polluting provinces of the central and western regions, but LTLs improve environmental quality only at the 70th, 80th, and 90th quantiles of CE in all provinces. Fourth, although renewable energy consumption inhibits carbon emissions in all provinces, oil prices, urbanization, trade openness, and foreign direct investments have heterogeneous effects on carbon emissions across the quantiles. Finally, our findings validate the EKC hypothesis only in the Eastern and Central regions. Accordingly, we propose policy implications for sustainable development in China.JEL classification: C40; G10; G20; Q20; Q54
Duplex real-time qPCR for Feline Herpesvirus-Ⅰ and Feline Parvovirus
Authors: Liu Lichun , Zhou Yuxia , Ru Yi , Tian Hong , Wang Xuezhi , Zhang Shidong , Cui Dongan , Xu Guowei , Li Jianxi , Sun Yan 
Published: May 2022

Abstract
To establish a rapid detection method for feline herpesvirus-Ⅰ (FHV-Ⅰ) and feline parvovirus (FPV), the specific primers and probes were designed according to the gB gene and VP1 gene of the highly conserved regions in FHV-Ⅰ and FPV strain. We optimized the reaction system and conditions of primers and probes, and a dual real-time fluorescent qPCR method that can simultaneously detect the above two viruses was successfully established. The standard curve of the method has an excellent linear relationship, and the correlation coefficient (R2) is above 0.99; the amplification efficiency is between 90% and 110%; the minimum detection limit is about 20 copies/μL; the coefficient of variation within and between groups is less than 2% And only positive for FHV-Ⅰ and FPV gene amplification, but negative for FCV, SVV, VSV, and other infectious disease-related pathogen amplification.
Fibroblasts And Mouse's Breast Cancer Cells Can Form Cellular Aggregates in Improved Soft Agar Culture Medium
Authors: Zhang Xiangnan , Liang Shuo , Wang Enze , Tao Ning 
Published: May 2022

Abstract
Purpose
Cancer-associated fibroblasts (CAFs) promote cancer cell growth, invasion and migration. In this research, we aimed to build cellular aggregates of mouse’s breast cancer cells (TS/A) and normal fibroblasts (LX-2) or CAFs (ME-iLX-2) in soft agar culture medium, verifying this co-culture model’s value in screening of anticancer drugs and preliminarily demonstrating the effect of CD44 in aggregates formation.
Methods
We improved soft agar culture medium to co-culture CAFs (NFs) and TS/A to build the cellular aggregates model, and compared the amount and area of different co-culture groups. To verify its value in screening of anticancer drugs, eugenol which has been demonstrated its anticancer effect was added into this model. The transcription of human CD44 was analyzed through Real-time quantitative PCR (RT-qPCR).
Result
TS/A-fibroblast cellular aggregates were formed in improved soft agar culture medium. What’s more, the amount and area of aggregates in TS/A-ME-iLX-2 co-culture group were significantly higher than in TS/A-LX-2 co-culture group. In co-culture group with eugenol, the amount and area of TS/A-ME-iLX-2 aggregates were lower than control group. The result of RT-qPCR showed that the transcription volume of human CD44 in TS/A-ME-iLX-2 aggregates was higher than in TS/A-LX-2 aggregates.
Conclusion
Co-culturing cellular aggregates of fibroblasts and TS/A was successfully formed in improved soft agar culture medium, and the amount and area of aggregates further confirmed CAFs’ promotion effect to cancer cells. Eugenol test showed its value in screening of anticancer drugs. RT-qPCR result demonstrated the important effect of CD44 in aggregates formation.