bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular vesicles derived from fibroblasts induced with or without high glucose exert opposite effects on wound healing and angiogenesis
Authors: Bian Xiaowei , Li Bingmin , Tang Haowen , Li Qiankun , Hu Wenzhi , Wei Qian , Ma Kui , Yang Yuguang , Li Haihong , Fu Xiaobing , Zhang Cuiping 
Published: November 2022

BackgroundCommunication between fibroblasts and endothelial cells is essential for skin wound repair and regeneration. Extracellular vesicles (EVs) are crucial for intracellular communication by transporting active molecules. However, whether EVs derived from diabetic fibroblasts can perform the nomal communication function is unclear. Here, we compared the effects of EVs from human skin fibroblasts (HSFs) induced with or without HG on the angiogenic function of endothelial cells and wound healing.MethodsWe first collected EVs from HSFs cultured with normal glucose concentration (NG-EVs) or with HG concentration (HG-EVs) and applied them to treat human umbilical vein endothelial cells (HUVECs). The cells were divided into three groups: control group, NG-EVs group, and HG-EVs group. We then examined the proliferation, migration, apoptosis, and tube formation of HUVECs. To illustrate the mechanism, the expression of β-catenin, GSK-3β, and p-GSK-3β was detected by western-blot. Finally, NG-EVs or HG-EVs were used to treat the wounds of mice to determine their role in wound closure.ResultsBy DNA content detection, Annexin V/PI staining, and EdU staining, we found that NG-EVs promoted HUVEC proliferation, while HG-EVs exhibited an opposite effect (p &lt; 0.05). Scratch assay and tube formation assay demonstrated that NG-EV promoted angiogenesis in vitro, while HG-EVs showed negative impact (p &lt; 0.05). The expressions of β-catenin and p-GSK-3β in HUVECs were enhanced by NG-EVs and decreased by HG-EVs (p &lt; 0.05). Additionally, the in vivo experiment demonstrated that NG-EVs effectively promoted wound healing by locally enhancing blood supply and angiogenesis. In contrast, HG-EVs leaded to delayed wound closure and reduced blood supply and angiogenesis (p &lt; 0.05).ConclusionNG-EVs and HG-EVs exert opposite effects on wound healing and angiogenesis possibly by regulating GSK-3β/β-catenin signaling pathway. This research may provide a new treatment strategy for wound healing and illustrate the mechanism for impaired angiogenesis in diabetics.
Childhood Socioeconomic Position and Cardiovascular Disease Among Older Women and Men: The Moderating Role of Parenthood Onset
Authors: Ortiz-Llorens Manuel , Cabib Ignacio , Bambs Claudia 
Published: November 2022

Objectives: Based on a life-course approach, the purpose of this study is to analyze how the age at the birth of a first child moderates the relationship between childhood socioeconomic position (SEP) and cardiovascular diseases (CVD) incidence in old age, separately for women and men.Methods: We used a rich and representative life history survey of people aged from 65 to 75 living in Santiago, Chile (n = 802), and weighted multivariate statistical models. Data collection process involved the use of face-to-face life history calendars, administered by well-trained interviewers.Results: Early motherhood increases the risk of suffering CVD among older women with a disadvantaged childhood SEP, while late motherhood decreases it. By contrast, early fatherhood decreases CVD risk among older men with an adverse childhood SEP, while late fatherhood increases it.Conclusion: Our findings about the moderating role of parenthood onset on CVD risk among older women and men with a disadvantaged childhood SEP contributes to public health reflections on unexplored cardiovascular risk factors, which lead to substantial changes in women’s and men’s life courses, and might optimize cardiovascular prevention strategies.
Prevalence of Long-COVID Among Low-Income and Marginalized Groups: Evidence From Israel
Authors: Heller Oren , Chun Yung , Shapira Stav , Troen Aron , Shlomo Yaniv , Acri Mary , Marotta Phillip , Kulkarni Saneel , Kinnison Brendan , Grinstein-Weiss Michal 
Published: November 2022

Objective: To identify the socioeconomic and demographic factors associated with the prevalence of self-reported long-COVID symptoms.Method: We examined the association between acute-COVID (SARS-CoV-2) and long-COVID symptoms, by a cross-sectional analysis of data obtained on a prospective online-survey, conducted from November to December 2021 on a nationally-representative sample of the Israeli population (N = 2,246).Results: Findings suggest that there is a greater likelihood of experiencing long-COVID symptoms among low-income and among marginalized groups. After controlling for demographic and socioeconomic attributes, those who had moderate/severe acute-COVID were 1.3 (p &lt; 0.05) times more likely to experience a long-term symptom and also reported more long-term symptoms (2.2 symptoms) than those who have not been infected (1.4 symptoms; p &lt; 0.01). Among the low-income group, a larger gap in symptom count was found between those who had moderate/severe acute-COVID (3.3 symptoms) and those who had not been infected (1.8 symptoms, p &lt; 0.05).Conclusion: Our findings highlight the importance of raising awareness of long-COVID among marginalized population groups, and to the therapeutic options available. Such efforts should be tailored and should consider the unique socioeconomic and cultural characteristics, as well as the preexisting low access to healthcare services among these groups.
Invasion and Colonization of Pathogenic Fusarium oxysporum R1 in Crocus sativus L. during Corm Rot Disease Progression
Authors: Bhagat Nancy , Magotra Shanu , Gupta Rikita , Sharma Shikha , Verma Sandhya , Verma Praveen Kumar , Ali Tahir , Shree Ankita , Vakhlu Jyoti 
Published: November 2022

The corm rot of saffron caused by Fusarium oxysporum (Fox) has been reported to be the most destructive fungal disease of the herb globally. The pathogen, Fusarium oxysporum R1 (Fox R1) isolated by our group from Kashmir, India, was found to be different from Fusarium oxysporum f.sp. gladioli commonly reported corm rot agent of saffron. In the present study, Fox R1 was further characterized using housekeeping genes and pathogenicity tests, as Fusarium oxysporum R1 f.sp. iridacearum race 4. Though Fox R1 invaded the saffron plant through both corm and roots, the corm was found to be the preferred site of infection. In addition, the route of pathogen movement wastracked by monitoring visual symptoms, semi-quantitative PCR, quantitative-PCR (q-PCR), real-time imaging of egfp-tagged Fusarium oxysporum R1, and Fox R1 load quantification. This study is the first study of its kind on the bidirectional pathogenesis from corm to roots and vice-versa, as the literature only reports unidirectional upward movement from roots to other parts of the plant. In addition, the colonization pattern of Fox R1 in saffron corms and roots was studied. The present study involved a systematic elucidation of the mode and mechanism of pathogenesis in the saffron Fusarium oxysporum strain R1 pathosystem.
SOBRE TERRITÓRIOS E LUGARIDADES
Authors: Holzer Werther 
Published: November 2022

O que pretendo neste artigo é discutir a microterritorialidade a partir do lugar. Entre os conceitos, ou essências espaciais, que se referem à matéria sólida que nos dá apoio, três são importantes na discussão que faço aqui: mundo, lugar e território. Eles delimitam modos de ser-no-mundo, do mais introspectivo e solitário, para o mais interativo e compartilhado. Somos seres-em-situação, o que significa que constituímos e desvelamos o mundo, a partir de nossa individualidade de ser. Lugares, por sua vez, só existem porque os seres humanos compartilham suas experiências. A geograficidade, que expressa a materialidade do espaço geográfico, é compartilhada em nossas vivências cotidianas com a lugaridade, que expressa exatamente essa relação dialógica dos seres em movimento com lugares e caminhos. A essência do território é a fronteira, o limite. Minha tese é de que a expressão mais visível da microterritorialidade é a lugaridade. Para estudarmos os territórios, precisamos estudar os lugares.
STRATEGI PEMBELAJARAN KOSAKATA MELALUI MEMBACA DI TINGKAT PENDIDIKAN SARJANA
Authors: Astutik Ari , Setyowati Yulis 
Published: November 2022

Mahasiswa di fakultas Program Sastra Inggris di Universitas Wijaya Putra, tampaknya mengembangkan setiap keterampilan bahasa secara terpisah khususnya keterampilan kosa kata (vocabulary), sehingga ini menghasilkan pembelajaran kosa kata yang kurang maksimal karena tidak sesuai dengan pengetahuan bacaan yang akan atau yang sedang mereka baca. Dengan mengembangkan strategi belajar kosakata melalui membaca, peneliti ingin mengarahkan mahasiswa agar mempunyai motivasi yang tinggi dalam membaca bermacam-macam literatur baik itu dalam bentuk buku ataupun jurnal ilmiah, karena dari membaca bahan-bahan bacaan tersebut maka akan meningkatkan keterampilan kosakata para mahasiswa secara bersamaan ketika proses membaca. Metode kualitatif digunakan untuk mengumpulkan data serta kuesioner dirancang untuk mahasiswa; beberapa kegiatan membaca dirancang untuk mengeksplorasi strategi paling umum yang digunakan mahasiswa program studi sastra Inggris. Pesertanya seluruh mahasiswa semester I tahun ajaran 2022/2023 fakultas sastra inggris. Mereka dipilih secara acak untuk melakukan kegiatan atau kuesioner, mereka berpartisipasi secara bebas dalam prosedur penelitian untuk mengetahui tentang pendekatan mereka terhadap pembelajaran (vocabulary) kosa kata melalui kegiatan membaca. Hasil dalam penelitian ini adalah kebanyakan mahasiswa memilih menggunakan kamus sebagai alat bantu dalam meningkatkan kosakata mereka. Dari tiga strategi belajar vocabulary (kosakata) yaitu menebak dari konteks bacaan, menganalisis afiks / awalan dan akar kata, serta yang terakhir adalah dengan menggunakan kamus. Strategi menggunakan kamus adalah strategi terbanyak yang dipilih mahasiswa dalam strategi belajar kosakata melalui membaca dengan total jumlah 59. Disusul dengan strategi yang ke dua yang dipilih mahasiswa adalah menebak dari konteks bacaan dengan total jumlah 54 dan strategi yang terakhir dipilih adalah menganalisiss afiks/awalan dan akar kata dengan total jumlah 4. Ketiga strategi belajar kosakata diatas digunakan untuk belajar kosakata dengan membaca bacaan dari tiga genre bacaan yang berbeda yakni academic reading, job related reading dan personal reading. Penelitian ini diharapkan bisa memberikan kontribusi untuk meningkatkan kesadaran tentang strategi yang dapat membantu mahasiswa mengembangkan pembelajaran kosakata melalui keterampilan membaca secara lebih efektif. Keuntungan berbahasa Inggris yang baik dan benar akan membawa para mahasiswa menuju kesejahteraan di dalam hidup. Dengan mempunyai skill berbahasa inggris yang baik maka mereka akan mendapatkan kepuasan ditempat kerja karena dapat meningkatkan jenjang karier nya serta berkesempatan untuk mendapatkan keuntungan secara finansial bahkan dapat mencapai jenjang karir yang lebih tinggi karena mereka dapat bekerjasama lebih mudah dengan perusahaan asing.
CRITICAL DISCOURSE ANALYSIS CLARIFICATION OF BEAR BRAND PRODUCT HOAX DURING THE COVID PANDEMIC
Authors: Agustina Maya , Prawiradiredja Sanhari , Nevretia Nevretia 
Published: November 2022

Berita hoaks terkait produk bear brand yang diklaim masyarakat dapat menyembuhkan seseorang yang terkena virus covid. Menggunakan teori analisis wacana kritis model Teun A. Van Dijk dengan menggunakan metode deskripsi kualitatif. Dengan objek dua media online dan tiga berita. Hasil penelitian ini menunjukkan adanya wacana didalam dua media online yaitu tempo.co dan kompas.com. Kedua objek media online tersebut dengan tiga jenis berita peneliti terdapat wacana di dalam teks berita yaitu informasi hoaks ini muncul karna dipicu oleh adanya video yang beredar di media sosial “video membagikan 11 tips cepat pulih yang terkena virus covid” dan juga “video berebut susu beruang dipusat perbelanjaan” hingga masyarakat membuat beragam asumsi hingga para pakar dan para ahli dari universitas IPB dan UGM memberikan komentar terkait kasus ini bahwa berita yang beredar di media sosial dan masyarakat itu tidak benar. Susu beruang tidak dapat mengobati, menyembuhkan atau bahkan memulihkan seseorang yang terkena covid-19. Kandungan nilai gizi yang ada di susu bear sama saja dengan produk susu lainnya dan mengkonsumsi produk susu beruang tidak cukup untuk meningkatkan kekebalan tubuh disamping itu diperlukan asupan pola makan yang sehat dan bergizi, menjaga kebugaran tubuh, dan langkah yang terakhir mematuhi protokol kesehatan dengan cara 5M yaitu mencuci tangan dengan sabun, memakai masker, menjaga jarak, menjauhi kerumunan, dan membatasi mobilisasi dan interaksi. Serta berita online tempo.co lebih tegas dalam memberikan judul berita sedangkan kompas.com memberikan judul berita lebih ke samar-samar sesuai dengan apa yang dirasakan oleh masyarakat.
Analysis of Students’ Cognitive Presence and Perception in a Custom-Designed Virtual Problem Based Learning Assignment
Authors: Choo Chee Yan , Fahrni Mathumalar Loganathan , Long Chiau Ming 
Published: November 2022

All the teaching and learning activities except laboratory-based practicals remained conducted virtually during the transition from the Covid-19 pandemic to the endemic phase. The problem-based assignment for Pharmaceutical Analysis was conducted virtually. This study aims to evaluate students’ level of engagement and perception of virtual engagement to complete an online problem-based assignment on real situations using the cooperative Jigsaw model. Jamboard was used as an online communication tool to connect the students and facilitators. The content of the written discussion posted on the Jamboard and the questionnaire survey were analysed to establish the level of engagement. The student’s perception of virtual engagement was based on a questionnaire survey using descriptive analysis. Analysis of the student’s opinion posted on the Jamboard showed the presence of cognitive, social, and teaching components in the level of engagement during the virtual discussion. The information from the internet is borderless and the facilitators need to be knowledgeable to explain, guide, and stimulate higher-order thinking among the students. With careful course design, the Jigsaw cooperative activity on real problem-based questions could use to facilitate collaborative problem-solving skills among the students.
Prevalence and overlap of potential embolic sources in embolic stroke of undetermined source: a retrospective cohort
Authors: Nagazi Mouna , Aissi Mouna , Achour Asma , Abdelali Mabrouk , Mhiri Mariem , Frih-Ayed Mahbouba 
Published: November 2022

Introduction: Embolic strokes of an undetermined source may be caused by various potential embolic sources, which can be better managed by anticoagulant or antiplatelet therapy. Identifying these sources may have diagnostic and therapeutic implications. Our objectives were to assess the prevalence and overlap of different potential embolic sources identified in a population of patients with embolic strokes of undetermined sources, and to evaluate the stroke recurrence rate according to the type and number of potential embolic sources.
Methods: We used data from consecutive patients with ischemic stroke admitted to the department of neurology in Fattouma Bourguiba hospital (Monastir, Tunisia), between January 2017 and December 2020. Patients who met the embolic strokes of undetermined source diagnostic criteria according to the criteria of the Cryptogenic Stroke “embolic strokes of undetermined source” International Working Group were selected. The presence of each potential embolic source was assessed, and patients were categorised according to the identified potential embolic sources. The main outcome was ischemic stroke recurrence, and it was collected prospectively during follow-up after the index stroke.
Results: Among 330 patients admitted between 2017 and 2020, 66 (20.6%) were classified as embolic strokes of undetermined source (68.2% were men, mean age 57 ±11 years). The three most prevalent potential embolic sources were atrial cardiopathy (N = 47/66; 71.2%), arterial atherosclerosis (N = 46/66; 69.7%) and left ventricular disease (N = 26/66; 39.4%). Most patients (N = 56/66; 84.8%) had ≥2 potential embolic sources. After 6-month of follow up, ischemic stroke recurrence occurred in 18 (27.3%) patients. In survival analysis, the type and the number of potential embolic sources were not statistically associated with stroke recurrence.
Conclusion: Most patients with embolic strokes of undetermined source had multiple potential embolic sources, which overlap considerably. The type and number of potential sources were not associated with stroke recurrence. This finding may explain the negative results of large trials of secondary prevention in the Embolic strokes of undetermined source population.
REPRODUCTIVE AND SOMATIC HEALTH OF WOMEN OF FERTILE AGE IN ECOLOGICALLY UNFAVORABLE REGIONS OF TURKESTAN REGION
Authors: Аязбеков А.К. , Беркинбаев Г.Д. , Нурхасимова Р.Г. , Яковлева Н.А. , Курманова А.М. , Аязбекова А.Б. , Андабасова А.С. 
Published: November 2022

Интенсивное опустынивание, устойчивые необратимые процессы деградации окружающей природной среды приводят к ухудшению условий жизни и росту заболеваемости людей. Проведен ретроспективный анализ соматического здоровья и репродуктивной функции женщин фертильного возраста, проживающих на территории Туркестанской области. В экологически неблагоприятной зоне (г. Кентау и прилегающих к нему населенных пунктах) за последние 30 лет (1989-2019) отмечен высокий уровень экстрагенитальной патологии у беременных, с максимальным уровнем среди городских женщин г. Кентау (1226,2 + 21,166 на 1000 жен.) в период с 2000-2004 гг. За последние 15 лет наблюдается рост показателя экстрагенитальной патологии среди сельских женщин экологически неблагоприятной зоны с максимальным значением в 2010-2014 гг. (1174,9+18,074). За последние 10 лет в предкризисной зоне Туркестанской области также отмечен высокий уровень экстрагенитальной патологии - данный показатель в г. Туркестан в 3,5 раза выше, а в сельской местности предкризисной зоны - в 2,3 раза, чем в г. Ленгер. Таким образом, в экологически неблагоприятных зонах Туркестанской области (г. Кентау и прилегающих к нему населенных пунктах) и предкризисных зонах (г. Туркестан и прилегающих к нему населенных пунктах) уровень частоты экстрагенитальной патологии высокий, что требует принятия комплекса мероприятий, включающих медико-социальные меры поддержки, направленных на повышение уровня репродуктивного здоровья, оздоровление женщин фертильного возраста и повышение демографического потенциала.
Intensive desertification and persistent irreversible processes of environmental degradation, deterioration of living conditions, and an increase in morbidity have caused new socio-economic and environmental situations that require legislative solutions and legal regulation of social protection measures for the population living in ecologically unfavorable areas. A retrospective analysis of violations of somatic health and reproductive function of women living in the territory of the Turkestan region, the city of Kentau, settlements adjacent to the city was carried out, based on retrospective data from primary records, official statistics. The data obtained indicates a significant spread among women in Kentau city. extragenital pathology, the maximum level among urban women was recorded in the period from 2000 to 2004 (1226.2 + 21.166 per 1000 women), then it tended to decrease, while remaining quite high (2015-2019 - 521, 4. + 20.689 per 1000 women). That is, at present, more than half of pregnant women in Kentau have a history of extragenital pathologies. Violations of the somatic status and reproductive function of women are additional medical and demographic indicators for assessing the environmental situation of the territory.
Evaluating a hospital’s carbon footprint – A method using energy, materials and financial data
Authors: Lum Brandon X. , Tay Hubert M. , Phang Rachel X. , Tan Steven B. , Liu Eugene H. 
Published: November 2022

Background: Healthcare systems have to prepare for climate change’s health impact, while reducing healthcare’s contribution to global warming. Most evaluations of healthcare’s greenhouse gas emissions involve national level methodologies.Objective: As sustainability metrics become a key factor in hospital management, the paper describes a method for quantifying emissions at a large tertiary care hospital in Singapore.Methods: Hospital operational and financial data was used to determine the greenhouse gas effect of the hospital. Emission factors from government and academic sources were used for on-site and purchased energy emissions. Spend based emission factors from the environmentally-extended multiregional input-output (EE-MRIO) Eora database were used for other indirect emissions. This provided the total carbon footprint across the various scopes.Results:The hospital had an annual carbon footprint of 245,962 tonnes of carbon dioxide equivalents (CO2e). Scope 1 emissions accounted for 4,223 tonnes of CO2e, scope 2 for 38,380 tonnes of CO2e and scope 3 for 165,190 tonnes of CO2e. Operating carbon totalled 207,793 tonnes of CO2e, and 38,169 tonnes of scope 3 CO2e was attributed to capital expenditure projects. Medical equipment, pharmaceutical supplies and electricity were the largest contributors to the hospital’s carbon footprint.Conclusions: Identifying key areas contributing to emissions can enable targeted approaches in reducing a hospital’s carbon footprint, better preparing the hospital as the carbon economy evolves to include the healthcare sector.
Una propuesta de capacitación docente basada en el uso de un software de geometría dinámica
Authors: Scaglia Sara , Götte Marcela 
Published: November 2022

En este trabajo describimos una propuesta de capacitación docente en el uso de un software de geometría dinámica. Este uso conlleva una reflexión en torno a algunos aspectos de la enseñanza de la geometría como la actuación docente en el aula y las características del conocimiento que construye el alumno, que difieren de los correspondientes a un tratamiento más tradicional.
Se describen algunas reflexiones teóricas relacionadas con el uso en clase de un software de este tipo y se presentan algunas actividades propuestas, así como la actividad diseñada para la acreditación del taller. Además, se analizan los resultados obtenidos a la luz de las consideraciones teóricas.
La representación dinámica que exhibe y la autonomía de acción que promueve el software de geometría dinámica justifican una reflexión en torno a su uso que debe ser considerada en la formación inicial y continua de los docentes de matemática.