bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dermoscopy of haemosiderotic/aneurysmal dermatofibroma: A morphological study of 110 cases
Authors: Zaballos Pedro , Álvarez‐Salafranca Marcial , Llambrich Àlex , Malvehy Josep , Taberner Rosa , Medina Carolina , Argenziano Giuseppe , Thomas Luc , Pizarro Ángel , del Pozo Luis Javier , Avilés Jose Antonio , Martin Jose María , Karaarslan Isil , Guionnet Neus , Bañuls José 
Published: October 2022

AbstractBackgroundHaemosiderotic and aneurysmal dermatofibromas are uncommon and frequently misdiagnosed lesions, which can be considered as different histopathological stages of the same tumour. A dermoscopic diagnosis testing accuracy has not been performed for these tumours to date.ObjectivesTo determine the diagnostic significance of dermoscopic structures and patterns associated with haemosiderotic/ aneurysmal dermatofibromas in a large series.MethodsDermoscopic images of histopathologically proven cases of 110 haemosiderotic/ aneurysmal dermatofibromas and 501 other tumours were collected. The frequency, sensitivity, specificity, positive predictive value and negative predictive value of the dermoscopic structures and patterns associated with these lesions were calculated.ResultsHaemosiderotic/ aneurysmal dermatofibromas are mostly symmetric lesions (86.5%), and a prominent homogeneous area was present in 100% of them. The presence of vascular structures was very common (86.4%), and dotted vessels were predominant (58.2%). Shiny white structures were seen in 85.5% of lesions, while a peripheral delicate pigment network was present in 69.1%. The most significant pattern was the one composed of a prominent homogeneous area and peripheral delicate pigment network, which showed a specificity of 100% with a relatively good sensitivity (69.1%). All the patterns containing a peripheral delicate pigment network showed very good specificities, positive predictive values and negative predictive values. Those patterns without a peripheral delicate pigment network showed the highest sensitivities, but they showed a significant overlap with other tumours, mainly with melanoma.ConclusionsDermoscopy is helpful in improving the diagnostic accuracy of haemosiderotic/ aneurysmal dermatofibromas. However, there is a considerable dermoscopic overlap between these tumours and melanoma, specifically when the peripheral delicate pigment network is absent.
Study on the microbial inactivation of whole hulless barley flour using a continuous instant pressure drop sterilizer
Authors: Chen Zhiying , Wang Aixia , Qin Wanyu , Xi Huihan , He Yue , Nie Mengzi , Liu Liya , Wang Lili , Bai Yajuan , Huang Yatao , Wang Fengzhong , Tong Li‐Tao 
Published: October 2022

AbstractThis work evaluated the microbial reduction and quality change of the whole hulless barley flour (WHBF) treated by a continuous instant pressure drop (CIPD) sterilizer. The microbial reduction of WHBF was proportional to steam‐material ratio under the conditions of 165°C, 0.2 MPa steam, and 0.2 MPa Laval nozzle, and the microbial reduction was 3.87 ± 0.09 log cfu/g with the steam‐material ratio of 3.0:1. The work was followed‐up with the quality testing of CIPD, UV‐C, hot air, and ozone treated WHBF. The microbial reduction of hot air, UV‐C, and ozone treated WHBF was 3.98 ± 0.09, 3.33 ± 0.04, and 3.68 ± 0.12 log cfu/g, respectively, while the CIPD treatment achieved similar bactericidal effect at a unit treatment time of 22 s. Moreover, there is no measurable quality degradation in CIPD treated WHBF compared with the untreated WHBF. These results showed CIPD sterilizer had a great potential for microbial inactivation of powdery foods industry.
Spirulina and açai as innovative ingredients in the development of gummy candies
Authors: Paternina Laura Patricia Rivera , Moraes Luiza , Santos Thaisa Duarte , de Morais Michele Greque , Costa Jorge Alberto Vieira 
Published: October 2022

AbstractThe gummy industry is showing a growing interest in adding novel ingredients to conventional formulations since consumers are looking for healthier and more nutritious products. Thus, this work aimed to develop sucrose‐free gummy candies without artificial food dyes and incorporated with Spirulina biomass and freeze‐dried açai pulp (FDAP) at concentrations of 1%, 3%, and 5%. Gummy candy enriched with 5% Spirulina (GSP5) showed the nutritional (increase of 36.7% and 414.3% in protein and mineral contents, respectively) and functional increments (0.081 mg GAE g−1 of phenolic compounds and 11.4% DPPH scavenging activity) when compared to control (GC; 0% Spirulina). Gummy candy added with 3% FDAP (GAC3) showed 1.7% lipids, 0.4% minerals, 0.190 mg GAE g−1 of phenolic compounds, and 27.0% DPPH scavenging activity. The acceptability index was satisfactory for both formulations GSP5 (78.7%) and GAC3 (83.8%). Therefore, Spirulina biomass and FDAP could be used as innovative ingredients in sucrose‐free gummies manufacturing.Novelty impact statementThe addition of Spirulina biomass and freeze‐dried açai pulp in gummy candies improved their nutritional and functional value. Spirulina biomass addition increased gummy candies protein and mineral content, whereas gummy candies enriched with freeze‐dried açai pulp exhibited antioxidant capacity up to 36% and the highest phenolic content. Besides, the sensory evaluation of gummy candies indicated a high acceptability index of around 80%.
A bZIP transcription factor (CiFD) regulates drought‐ and low‐temperature‐induced flowering by alternative splicing in citrus
Authors: Ye Li‐Xia , Wu Yan‐Mei , Zhang Jin‐Xia , Zhang Jin‐Xin , Zhou Huan , Zeng Ren‐Fang , Zheng Wei‐Xuan , Qiu Mei‐Qi , Zhou Jing‐Jing , Xie Zong‐Zhou , Hu Chun‐Gen , Zhang Jin‐Zhi 
Published: October 2022

ABSTRACTDrought and low temperature are two key environmental factors that induce adult citrus flowering. However, the underlying regulation mechanism is poorly understood. The bZIP transcription factor FD is a key component of the florigen activation complex (FAC) which is composed of FLOWERING LOCUS T (FT), FD, and 14‐3‐3 proteins. In this study, isolation and characterization of CiFD in citrus found that there was alternative splicing (AS) of CiFD, forming two different proteins (CiFDα and CiFDβ). Further investigation found that their expression patterns were similar in different tissues of citrus, but the subcellular localization and transcriptional activity were different. Overexpression of the CiFD DNA sequence (CiFD‐DNA), CiFDα, or CiFDβ in tobacco and citrus showed early flowering, and CiFD‐DNA transgenic plants were the earliest, followed by CiFDβ and CiFDα. Interestingly, CiFDα and CiFDβ were induced by low temperature and drought, respectively. Further analysis showed that CiFDα can form a FAC complex with CiFT, Ci14‐3‐3, and then bind to the citrus APETALA1 (CiAP1) promoter and promote its expression. However, CiFDβ can directly bind to the CiAP1 promoter independently of CiFT and Ci14‐3‐3. These results showed that CiFDβ can form a more direct and simplified pathway that is independent of the FAC complex to regulate drought‐induced flowering through AS. In addition, a bHLH transcription factor (CibHLH96) binds to CiFD promoter and promotes the expression of CiFD under drought condition. Transgenic analysis found that CibHLH96 can promote flowering in transgenic tobacco. These results suggest that CiFD is involved in drought‐ and low‐temperature‐induced citrus flowering through different regulatory patterns.
Cannabinoids and synthetic cannabinoids as a cause of death: Trends and their healthcare implications
Authors: Suriaga Armiel , Tappen Ruth M. , Aston Elizabeth R. , Chiang‐Hanisko Lenny , Newman David 
Published: October 2022

AbstractIntroductionCannabis remains one of the most widely used illicit substances globally, with 188 million users in 2017. In the United States, nearly 50 million people are reported to have used cannabis in 2020. More research is needed because of the dramatic increase in cannabis use and the perception that its use has minimal risk.DesignThe researchers used a retrospective design for this study.MethodsWe used the Florida Department of Law Enforcement data from 2014 to 2020 for this study. We used descriptive statistics to report the characteristics of decedents whose cause of death (COD) was associated with cannabinoid (CB) and synthetic cannabinoid (SC) use. We used a general linear model with repeated measures to examine CB and SC death rate trends.ResultsA total of 386 decedents' COD in Florida was associated with CB and SC use. Nearly 28% of decedents were 45–54 years, male (87.8%), and non‐Hispanic whites (65.3%). One hundred percent of CB‐related decedents died in urban counties. In rural counties, SC decedents accounted for 28.3% of deaths. Of decedents in rural counties, 39.9% were African American. Most decedents (with CB and SC use) died from accidents (98.7%), with 12.6% of cases involving cardiovascular‐related illnesses.ConclusionCBs and SCs as a COD pose a legitimate health problem to society. More people ages 45–54 died from CBs and SCs. Drug intoxications (from CBs and SCs) and motor vehicle collisions accounted for most of the accidents reported while under the influence of CBs and SCs. While most decedents from both CBs and SCs were non‐Hispanic whites, a substantial proportion of African Americans died from SCs as a COD in rural counties. It is important that the public become aware of the risks for adverse effects of CB and SC. The public needs to be aware that CB and SC use can exacerbate cardiac‐related conditions.Clinical RelevanceThis study has clinical relevance to patient safety. CB and SC use contributes to motor vehicle accidents and can cause adverse effects including death.
Guidelines for obstetrical practice in Japan: Japan Society of Obstetrics and Gynecology and Japan Association of Obstetricians and Gynecologists 2020 edition
Authors: Itakura Atsuo , Satoh Shoji , Aoki Shigeru , Fukushima Kotaro , Hasegawa Junichi , Hyodo Hironobu , Kamei Yoshimasa , Kondoh Eiji , Makino Shintaro , Matsuoka Ryu , Morikawa Mamoru , Nagamatsu Takeshi , Nakata Masahiko , Naruse Katsuhiko , Nishigori Hidekazu , Nishiguchi Tomizo , Obata‐Yasuoka Mana , Ohno Yasumasa , Oura Kuniaki , Shimoya Koichiro , Shiozaki Arihiro , Suzuki Shunji , Tanaka Kanji , Yoshida Shiro , Kudo Yoshiki , Maeda Tsugio , Shozu Makio 
Published: October 2022

AbstractThe 2017 clinical guidelines for obstetrical practice by the Japan Society of Obstetrics and Gynecology and the Japan Association of Obstetricians and Gynecologists were revised and published as the 2020 edition (in Japanese). The aim of these guidelines is to present appropriate standard obstetric diagnosis and management procedures that have reached consensus among Japanese obstetricians. The 2020 guidelines include 113 clinical questions and an appendix, followed by answers (CQ&A; originally 112 in the 2017 edition), a discussion, list of references, and some tables and figures presenting common problems and questions encountered in obstetrical practice. Each answer comes with a recommendation level of A, B, or C and has been prepared based principally on evidence or a consensus among Japanese obstetricians in situations where “evidence” is weak or lacking. Answers with a recommendation level of A or B represent current standard care practices in Japan. All 113 CQ&As and the appendix are presented here to promote a better understanding of the current standard care practices for pregnant and lactating women in Japan.
The effect of Porphyromonas gingivalis on the gut microbiome of mice in relation to aging
Authors: Giri Sarita , Uehara Osamu , Takada Ayuko , Paudel Durga , Morikawa Tetsuro , Arakawa Toshiya , Nagasawa Toshiyuki , Abiko Yoshihiro , Furuichi Yasushi 
Published: October 2022

AbstractBackground and ObjectiveThe translocation of oral bacteria, including Porphyromonas gingivalis, to the gut has been shown to alter gut microbiome. However, the effect of P. gingivalis on gut microbiome in relation to aging has not been demonstrated. We hypothesize that P. gingivalis has more detrimental effect on gut environment with increased age. The objective of this study is to investigate the effect of P. gingivalis on gut environment using aged mice.Materials and MethodsC57BL/6J mice aged 4 weeks (young) or 76 weeks (old) were divided into four groups: control‐young, control‐old, P. gingivalis‐administered young, and P. gingivalis‐administered old. P. gingivalis was orally administered thrice weekly for 5 weeks. At 30 days after the last P. gingivalis administration, 16S rRNA sequencing was performed to study the gut microbiome. The mRNA and protein expression of intestinal junctional barrier molecules and the levels of the inflammatory cytokines IL‐1β and TNF‐α in the serum were evaluated.ResultsSignificant differences in the gut microbiomes between the groups, in terms of taxonomic abundance, bacterial diversity, and predicted metagenome function, were observed. A significant reduction in the alpha diversity and in the abundance of beneficial bacteria, such as Akkermansia and Clostridiaceae, in the P. gingivalis‐administered old mice was observed. The mRNA and protein levels of Claudin‐1 and Claudin‐2 in the intestine were significantly elevated, while E‐cadherin was significantly downregulated in the P. gingivalis‐administered old mice, as were the serum levels of IL‐1β and TNF‐α.ConclusionThe effect of P. gingivalis on the gut environment is more pronounced in old mice than in young mice.
Sequential hybridization may have facilitated ecological transitions in the Southwestern pinyon pine syngameon
Authors: Buck Ryan , Ortega‐Del Vecchyo Diego , Gehring Catherine , Michelson Rhett , Flores‐Rentería Dulce , Klein Barbara , Whipple Amy V. , Flores‐Rentería Lluvia 
Published: October 2022

SummaryMultispecies interbreeding networks, or syngameons, have been increasingly reported in natural systems. However, the formation, structure, and maintenance of syngameons have received little attention. Through gene flow, syngameons can increase genetic diversity, facilitate the colonization of new environments, and contribute to hybrid speciation.In this study, we evaluated the history, patterns, and consequences of hybridization in a pinyon pine syngameon using morphological and genomic data to assess genetic structure, demographic history, and geographic and climatic data to determine niche differentiation.We demonstrated thatPinus edulis, a dominant species in the Southwestern US and a barometer of climate change, is a core participant in the syngameon, involved in the formation of two drought‐adapted hybrid lineages including the parapatric and taxonomically controversialfallax‐type. We found that species remain morphologically and genetically distinct at range cores, maintaining species boundaries while undergoing extensive gene flow in areas of sympatry at range peripheries.Our study shows that sequential hybridization may have caused relatively rapid speciation and facilitated the colonization of different niches, resulting in the rapid formation of two new lineages. Participation in the syngameon may allow adaptive traits to be introgressed across species barriers and provide the changes needed to survive future climate scenarios.
Computational modeling to assist in the discovery of supramolecular materials
Authors: Jelfs Kim E. 
Published: October 2022

AbstractComputational modeling is increasingly used to assist in the discovery of supramolecular materials. Supramolecular materials are typically primarily built from organic components that are self‐assembled through noncovalent bonding and have potential applications, including in selective binding, sorption, molecular separations, catalysis, optoelectronics, sensing, and as molecular machines. In this review, the key areas where computational prediction can assist in the discovery of supramolecular materials, including in structure prediction, property prediction, and the prediction of how to synthesize a hypothetical material are discussed, before exploring the potential impact of artificial intelligence techniques on the field. Throughout, the importance of close integration with experimental materials discovery programs will be highlighted. A series of case studies from the author's work across some different supramolecular material classes will be discussed, before finishing with a discussion of the outlook for the field.
Posterior Pelvic Tilt in the Standing Position Might Be Associated with Collapse Progression in Post‐Collapse Stage Osteonecrosis of the Femoral Head
Authors: Xu Mingjian , Motomura Goro , Ikemura Satoshi , Yamaguchi Ryosuke , Kawano Koichiro , Yamamoto Noriko , Tanaka Hidenao , Ayabe Yusuke , Nakashima Yasuharu 
Published: October 2022

ObjectiveExcessive pelvic tilt has been reported to impair the biomechanical loading of the hip joint. However, the influence of pelvic tilt in osteonecrosis of the femoral head (ONFH) remains unclear. This study aims to assess whether sagittal pelvic posture in the standing position correlates with progression of femoral head collapse in post‐collapse stage ONFH.MethodsThis is a single‐center retrospective study. We investigated 107 patients (107 hips; 73 males and 34 females; mean age, 48 years) diagnosed with Association of Research Circulation Osseous (ARCO) stage III ONFH at the first visit and who subsequently underwent surgical treatment in our institution from July 2016 to December 2020. The sagittal pelvic posture in the standing position before surgery was quantified as the angle formed by the anterior pelvic plane and the vertical z‐axis in the sagittal view (APP angle). An APP angle <0° indicated posterior pelvic tilt. Progression of femoral head collapse was calculated as collapse speed. The following factors potentially associated with collapse speed were evaluated by exploratory data analysis followed with multiple linear regression analysis: sex, age, BMI, etiology, pelvic incidence, contralateral hip condition, time interval between the first visit and surgery, size of necrotic lesion, location of necrotic lesion, and APP angle.ResultsAs ONFH progressed from ARCO stage IIIA to stage IV, APP angle decreased significantly and continuously (stage IIIA, −0.2° ± 5.5°; stage IIIB, −3.7° ± 5.8°; stage IV, −7.1° ± 6.4°). The factors significantly associated with collapse speed were size of necrotic lesion (p = 0.0079), location of necrotic lesion (p = 0.0190), and APP angle (p < 0.0001). APP angle showed a negative correlation with collapse speed (r = −0.40, p < 0.0001). After stratifying by size of necrotic lesion (<50% and ≥50% involvement) and location of necrotic lesion (JIC type C1 and C2), a significant negative correlation was observed between APP angle and collapse speed in each group (JIC type C1 with <50% involvement, r = −0.69, p < 0.0001; JIC type C1 with ≥50% involvement, r = −0.58, p = 0.0475; JIC type C2 with <50% involvement, r = −0.51, p = 0.0124; JIC type C2 with ≥50% involvement, r = −0.39, p = 0.0286).ConclusionsOur results suggest that posterior pelvic tilt in the standing position occurred as ONFH progressed from ARCO stage IIIA to stage IV, which might be associated with progression of femoral head collapse in ONFH.
Surgical Transepicondylar Axis Is Not a Reliable Reference when there Was Lateral Femoral Bowing
Authors: Liu LiMing , Lei Kai , Guo Lin , Chen Xin , Yang PengFei , Fu DeJie , Xiong Ran , Yang Liu 
Published: October 2022

ObjectiveThe surgical transepicondylar axis (sTEA) is believed to be a consistent reference for femoral rotation axis, and the reliability of its orientation seriously affects the accuracy and outcome of total knee arthroplasty (TKA). This study was designed to investigate the relationship between the orientation of sTEA and femoral bowing angle (FBA) and posterior condylar line (PCL) using three‐dimensional (3D) computed tomography (CT) reconstruction models to verify its reliability.MethodsThis study retrospectively collected lower extremity images of 443 southern Chinese osteoarthritic patients (347 women, 96 men; 234 left, 209 right; mean age 66.5 ± 9.3 years) from August 2016 to June 2018. The hip–knee‐ankle angle (HKA) was measured on anteroposterior weight‐bearing full lower extremity standing radiographs. Measurements on 3D CT models of the femurs included lateral angle between the femoral mechanical axis and sTEA coronal angle, angle between sTEA and distal joint line (distal condylar axis angle, DCA); angle between sTEA and PCL (sTEA axial angle); angle between anatomical axis of proximal femur and anatomical axis of distal femur in the plane they form (actual FBA) and its projection on the coronal (lateral FBA) and sagittal (anterior FBA) planes. The correlations between sTEA coronal angle, sTEA axial angle and actual FBA, lateral FBA, anterior FBA, HKA, DCA were explored using the Spearman correlation test.ResultsThe mean value of actual FBA is (14.4 ± 3.6)°, of lateral FBA is (6.0 ± 4.0)°, and of anterior FBA is (12.7 ± 3.0)°. The mean value of sTEA coronal angle is (88.7 ± 3.6)°, of sTEA axial angle is (2.1 ± 2.8)°. The sTEA coronal angle was positively correlated with actual FBA (r = 0.320, P < 0.01), lateral FBA (r = 0.448, P < 0.01), anterior FBA (r = 0.113, P < 0.05), HKA (r = 0.482, P < 0.01) and DCA (r = 0.486, P < 0.01). The sTEA axial angle was positively correlated with DCA (r = 0.168, P < 0.01), but not significantly correlated with all FBAs and HKA (NS).ConclusionThe sTEA cannot be used as a stable reference when there was an obvious femoral bowing deformity. As the lateral femoral bowing increases, the orientation of sTEA becomes more varus, no matter the knee is varus or valgus.
Effect of a low seed lignin content on seed yield and quality in winter oilseed rape (Brassica napus)
Authors: Holzenkamp Karin , Link Wolfgang , Möllers Christian 
Published: October 2022

AbstractIncreasing demand for vegetable oil and protein requires breeding efforts to enhance the yield of oilseed rape. This can be achieved by reducing seed cellulose, hemicellulose and lignin content. The objective of the study was to analyse seed yield and important agronomic traits of two distinct bulks of genotypes from two crosses, with low (6%) and high seed lignin content (10%). The two genotype bulks were formed by mixing seeds of 12 doubled haploid lines with either low or high lignin content. The genotypes were selected from two large doubled haploid populations based on lignin content, flowering time and plant height. The bulks were field‐tested in replicated yield trials. Seed quality analysis confirmed significant differences in seed lignin content between the two bulks. Seed oil content was moderately higher in the low lignin bulks of both crosses. Seed, oil and protein yield in both crosses were moderately to significantly higher in the low lignin bulks. Further breeding for reduced fibre content will help increase seed oil and protein content.