bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deletion of PLD2 Alleviates LPS Induced Acute Lung Injury by Inhibiting STAT3 Phosphorylation and Regulating Endothelial Tight Junctions
Authors: Qian Tiantian , Qi Boyang , Fei Yuxin , Li Jun , Luo Liqing , Song Yutong , Sheng Shurui , Lv Bingjie , Huang Xiao , Wang Xiaozhi 
Published: June 2022

Abstract
Background: Acute respiratory distress syndrome (ARDS) is the leading cause of acute respiratory failure. Endothelial cell damage and increased permeability are critical in developing acute lung injury (ALI). Phospholipase D2 (PLD2) and its metabolite phosphatidic acid (PA) regulate many physiological activities in cells, disrupting cell barrier integrity. However, the exact mechanism remains unexplored. Therefore, we elucidated the role and action mechanism of PLD2 in lipopolysaccharide (LPS)-induced endothelial cell integrity.Methods: We used wild-type (WT) and PLD2 knockout (PLD2-/-) mouse models of LPS-induced ALI. Altered pulmonary endothelial permeability was assessed using histopathological analysis. The relative permeability of the pulmonary vascular endothelial cell culture model was assessed using transwell chamber assays for FITC-dextran and TEER transcellular resistance. The PA content, a downstream product of PLD2, was detected using ELISA, and the mRNA expression of tight junction proteins was detected by real-time quantitative polymerase chain reaction (RT-PCR). Changes in tight junction protein levels and STAT3 pathway phosphorylation, a critical endothelial barrier component, were assessed using immunofluorescence and western blotting analyses in vivo and in vitro.Results: PLD2-/- could significantly improve the histopathological changes, lung wet-dry ratio, and endothelial cell permeability in LPS-induced ALI mice. Simultaneously, PLD2-/- could reduce PA-induced STAT3 phosphorylation, resulting in reduced endothelial tight junction degradation.Conclusion: Loss or inhibition of PLD2 significantly inhibits tight junction injury and alleviates lung injury. LPS-induced PA production leads to STAT3 pathway phosphorylation and tight junction protein targeting, resulting in increased barrier permeability. Collectively, PA is crucial in ARDS pathogenesis.
Suppression of Heterotopic Ossification in Fibrodysplasia Ossificans Progressiva Using AAV Gene Delivery
Authors: Yang Yeon-Suk , Kim Jung-Min , Xie Jun , Chaugule Sachin , Lin Chujiao , Ma Hong , Hsiao Edward , Hong Jaehyoung , Chun Hyunho , Shore Eileen , Kaplan Frederick , Gao Guangping , Shim Jae-Hyuck 
Published: June 2022

Abstract
Progressive heterotopic ossification (HO) is the most disabling and defining feature of fibrodysplasia ossificans progressiva (FOP), an ultra-rare genetic disorder. More than 95% of FOP patients harbor a heterozygous activating mutation (c.617G>A;p.R206H) in the Activin A Receptor Type 1 (ACVR1). Currently, there are no definitive preventions or treatments. Gene therapy is thus an attractive treatment strategy. Here, we identify recombinant adeno-associated virus 9 (rAAV9) as the most effective serotype for transduction of the major cells-of-origin of HO in soft tissues. Using AAV-mediated gene delivery, three gene therapy approaches for FOP, including gene replacement by expression of codon-optimized human ACVR1 (ACVR1OPT), ACVR1R206H allele-specific silencing by AAV-compatible artificial miRNA (amiR, amiR-ACVR1R206H), and a combination of gene replacement and silencing (ACVR1OPT;amiR-ACVR1R206H), were developed. AAV gene therapy ablated aberrant Activin A signaling and chondrogenic and osteogenic differentiation of mouse skeletal cells harboring a conditional knock-in allele of human ACVR1R206H (Acvr1(R206H)KI) and human FOP patient-derived induced pluripotent stem cells (iPSCs). Accordingly, trauma-induced HO was markedly reduced in Acvr1(R206H)KI mice treated locally at early adulthood or systemically at birth. These mice displayed a significant decrease in endochondral bone formation in injured muscle while inflammation and fibroproliferation responses to injury remained largely intact. Remarkably, spontaneous HO in juvenile and adult Acvr1(R206H)KI mice was also substantially decreased by AAV gene therapy. Compared to gene replacement or silencing, combination gene therapy was the most potent in preventing both traumatic and spontaneous HO in FOP mice. Collectively, our results identify novel gene therapeutics that can prevent disabling HO in FOP mice and provide evidence to support clinical translation to FOP patients.
A dictionary-matrix separation and Viterbi decoding for Miller code in RFID tag collision
Authors: Zeng Yu , Ding Hongwei 
Published: June 2022

Abstract
With the help of perception technologies such as Radio Frequency Identification (RFID), information interaction will expand from communication devices to various non-communication items in daily life, and the era of ubiquitous personal communication networks may come. In an Ultra High Frequency (UHF) RFID system, however, signal collision often occurs during multiple tags identification. The collision resolution across a Media Access Control (MAC) layer and a physical layer can significantly improve communication efficiency. As a delay code, Miller code is often used in the UHF RFID system. Because its baseband signal has more subcarriers, it has higher anti-noise performance. This paper adopt the cross-layer resolution and proposes a new collision separation and decoding algorithm for the Miller code of the RFID system. This algorithm separates the collision signal by clustering and dictionary matrix firstly, and then decode the separated signal by Viterbi method. In this simulation, we generate a set of simulation data to evaluate the proposed algorithm. The simulation results show that the separation efficiency of the proposed algorithm is about 25% higher than that of traditional methods. Furthermore, we evaluate the performance of the proposed algorithm in this measured data, and obtain 8% higher system throughput than a traditional dynamic ALOHA system.
Decoupling and scenario analysis of economy-emissions pattern in China’s 30 provinces
Authors: Chen Jiayang , Yuan Rong , Zheng Shenglin 
Published: June 2022

Abstract
The tension between reducing CO2 emissions and economic growth has become increasingly prominent in recent years, while China is vigorously promoting ecological civilization to achieve sustainable development. We analyze the decoupling of emissions at the national and provincial levels of the Chinese economy from the perspective of historical patterns and current drivers from 1997 to 2019. Also, we developed three scenarios (i.e., pessimistic, median, and optimistic scenarios) to analyze the impact of decoupling relationship changes. We find that China’s national decoupling relationship has eased since 1997, but it has not yet reached the ideal state, with provinces mainly exhibiting weak decoupling. The EKC hypothesis is tested for the whole country and 30 provinces and find that 15 provinces have two turning points, 13 provinces have one turning point, and the others have no turning point. Based on the scenario analysis, the total emissions in the Pessimistic Scenario (S1) without any improvement of decoupling would increase by 73.97% compared to the level of 2019. However, the total emissions in the Optimistic Scenario (S3), in which all provinces obtained strong decoupling, are almost half of the level of 2019. This is mainly from the reduction of emissions in the western less developed regions (e.g., Shanxi, Inner Mongolia and Xinjiang) and developed coastal regions (e.g., Jiangsu and Shandong). On the basis of the results of factor analysis, we put forward policy recommendations for expand electrification, optimize industrial structure, and promote technological innovation.
The interaction effects of degradable microplastics and Cd to Folsomia candida soil collembolan
Authors: Liu Guoqiang , Gu Xuanzhu , Wu Jing , Li Haidong , Su Lianghu , Chen Mei , Chen Sujuan , Liu Yonghua 
Published: June 2022

Abstract
Microplastics have become a major safety concern for soil ecosystem, but in real field soil conditions, multiple chemicals exposure may be the real scenario for soil biota. The co-occurrence of microplastics and Cadmium (Cd) is common in soils, which may pose a potential risks to soil ecosystems. Meanwhile, although degradable microplastics are suggested to use, the potential effects on soil ecosystems are unknown. Therefore, a standard soil animal collembolan Folsomia candida (F. candida) was used to evaluation the single and interaction effects of degradable microplastics and Cd. The results showed that single and co-microplastics and Cd all had negative influences on the survival, reproduction and growth of F. candida, and the effects intensified with microplastics concentrations. But combined microplastic and Cd alleviated the toxicity of single chemical on F. candida at lower microplastics concentrations. Biochemical assays on antioxidant enzymes had the same results. Toxicity endpoints reproduction was not more sensitive than survival and growth. Antioxidant enzymes CAT and POD was more sensitive than SOD, thus may be promising biomarkers on soil collembolan for soil microplastics exposure.
Impacts of mir146a genotypes to bladder cancer risk in Taiwan
Authors: Chang Wen-Shin , Liao Cheng-Hsi , Chen Chao-Hsuan , Wang Bo-Ren , Wang Zhi-Hong , Yang Jai-Sing , Wang Yun-Chi , Gu Jian , Bau Da-Tian , Tsai Chia-Wen 
Published: June 2022

Abstract
We aimed to examine the associations of the genotypes for mir146a and mir196a with bladder cancer (BLCA) risk in Taiwan. Mir146a rs2910164 and mir196a rs11614913 genotypes among 375 bladder cancer patients and 375 healthy subjects were determined using the typical PCR-RFLP methodology, and their associations and interactions with age, gender, smoking and alcohol drinking status on BLCA risk were evaluated. Simultaneously, the serum expression level of mir146a was measured using quantitative RT-PCR. The results showed that the partitions of CC, CG and GG of mir146a genotypes were 31.7, 45.6 and 22.7% in the control group and 21.9, 44.3 and 33.8% in the case group, respectively. In logistic regression analysis, the heterozygous variant genotype CG carriers were at a bolder line significantly increased BLCA risk (OR = 1.41, 95% CI = 0.99–2.01) and the homozygous variant GG carriers were at a 2.17-fold increased BLCA risk (OR = 2.17, 95%CI = 1.46–3.21). Moreover, the GG/CG genotype carriers have significantly higher serum levels of mir146a than the CC genotype (p < 0.0001), supporting a genotype-phenotype correlation. The mir196a rs11614913 SNP were not associated with BLCA risk. In conclusion, genotypes of mir146a rs2910164 may serve as a risk prediction marker for BLCA.
Diurnal activity budget and feeding activity of grivet monkeys (Chlorocebus aethiops) in Zegie Peninsula, Northern Ethiopia
Authors: Yitayih Yibelu , Asmare Esubalew , Mola Misganaw 
Published: June 2022

Abstract
Background
Grivet monkeys (Chlorocebus aethiops) are an Old World monkey with long white tufts of hair along the sides of the face. We collected data on the activity patterns of the members of the 2 neighboring focal groups on an average of 4 day per month from August 2021 to March 2022 covering both the wet and dry seasons. Scan sampling method at 15 minutes interval was used to collect data on diurnal activity patterns and feeding ecology of grivet monkeys.
Results
A total of 16707 individual behavioral scans were recorded for a total of 24 days; 12 days during the wet season (August, September and October) and 12 days during the dry season (January, February and March). Grivet monkeys spent 39.4% of time feeding, 29.6% moving, 19.9% resting, 4.4% grooming, 3.1% social play, 2.4% aggression, and 1% for other activities. The overall diet of grivet monkeys was dominated by fruit (61.2%), while young leaf accounted (22%), young shoot (8%), bark (4.6%), animal prey (2.9%), and others (1.1%). A total of 22 plant species were consumed by grivet monkeys in the study area. The top four plant species namely Mimuscops kummel, Ficus sur, Strychanoss pinosa and Ficus vasta accounted for more than 73% of their plant diet. According to the total percentage contribution of the plant food items eaten, Mimuscops kummel was the most frequently consumed species which accounted about 29%, Ficus sur was 17%, Strychanoss pinosa 16%, and Ficus vasta was 11%.
Conclusion
Grivet monkeys spent more time for feeding followed by, moving, resting, grooming, social play, aggression and other activities. Grivet monkeys are mostly feed on fruits and they also feed on young leaves, shoots, bark, animal preys and other food items.
Effect of Preferential Flow on the Fate of Nitrate in Coastal Unconfined Aquifer
Authors: Gao Chao , Kong Jun , Wang Jun , Zhang Jinming , Chen Weilun , Hua Guofen 
Published: June 2022

Abstract
This study examined the influence of preferential flow (PF) on salinity change, pore water flows, and solute transport reaction in variable saturation and variable density coastal aquifers. The 2-D unconfined aquifer model established was based on the finite element software COMSOL by coupling the dynamic and chemical processes together. The simulated results showed that PF affect groundwater flow, leading to a more complicated mixing process. PF was found to effectively reduce the length of seawater intrusion. The deeper the depth of PF, the more significant the effect. Owing to this complex and dynamic process, nitrification mainly took place in the shallower zone adjacent to the coast, where oxic seawater and groundwater interact intensively. Denitrification, on the other hand, was dominant in the middle zone of the aquifer, where the oxygen was relatively lesser. Further quantitative analysis revealed an increase in the intensity of nitrification and dissolved oxygen inflow flux with PF depth. This also led to a decrease in the nitrate (NO3−) removal efficiency. This phenomenon usually occurs on coasts where biological caves are abundant. The results also offer significant implications for designing engineering measures to mitigate saltwater intrusion and groundwater quality management in coastal zones.
Usefulness of PIVKA-II for Monitoring After Liver Transplantation in Patients With Hepatocellular Carcinoma
Authors: LÓPEZ FRANCISCO VILLALBA , MATEOS LUIS FRANCISCO SÁENZ , LORENCIO MARIA ISABEL SÁNCHEZ , GARCÍA VIRGINIA DE LA ORDEN , GAGO FELIPE ALCONCHEL , CAMPOS PEDRO ANTONIO CASCALES , BERNARDO CARMEN GARCÍA , VELASCO JOSÉ ANTONIO NOGUERA , MAZO ALBERTO BAROJA , ROMERO PABLO RAMÍREZ 
Published: June 2022

Abstract
The high morbidity and mortality of hepatocellular carcinoma (HCC) has encouraged the search for new biomarkers to be used alongside alpha-foetoprotein (AFP) and imaging tests. The aim of this study was to evaluate the clinical contribution of protein induced by vitamin K absence or antagonist-II (PIVKA-II) for HCC monitoring after liver transplantation (LT) and compare it with AFP, a routinely used tumour marker. A total of 46 HCC patients (Milan criteria) were enrolled in this study. Serum levels of PIVKA-II and AFP were measured before and after transplantation. Clinical features were determined for all the patients that were included. Significant correlations were found between PIVKA-II expression levels and some clinicopathological features, such as tumour size and number of pre-transplant transarterial chemoembolizations (TACEs). Serum levels of PIVKA-II and AFP decreased significantly after LT and increased in patients with tumour recurrence. Serum PIVKA-II levels may play an important role in predicting disease severity. Furthermore, monitoring PIVKA-II levels in HCC transplant recipients reflects the tumor early recurrence after transplantation and could be used, complementing AFP and imaging tests, as a novel biomarker of this pathology.
I-κB kinase-ε deficiency attenuates the development of Doxorubicin-induced dilated cardiomyopathy
Authors: Liu Yafeng , Xu Yueyue , Yao Yiwei , Cao Yide , Chen Ganyi , Cai Yuchen , Chen Wen , Chen Xin , Qiu Zhibing 
Published: June 2022

Abstract
Dilated cardiomyopathy (DCM) can lead to heart expansion and severe heart failure, but its specific pathogenesis is still elusive. In many cardiovascular diseases, I-κB kinase-ε (IKKε) has been recognized as a pro-inflammatory molecule. In this study, we aimed to determine the effect of IKKε on Dox-induced cardiomyopathy in mice. Wild-type mice (WT, n=14) and IKKε knockout mice (IKKε-KO, n=14) were generated and intraperitoneally injected with Dox or Saline five times in 30 days. Our results demonstrated that compared to WT mice, IKKε-KO mice exhibited attenuation of dilated cardiomyopathy-related morphological changes and alleviation of heart failure after Dox was injected.Additionally, compared to the WT mice after Dox-injected, the expression of fibrosis and proinflammatory were decreased in IKKε-KO mice, and the expression of cardiac gap junction proteins were much higher in IKKε-KO mice. Further testing found that pyroptosis and apoptosis in myocardium were also ameliorated in IKKε-KO mice compared to WT mice after Dox was injected. Mechanistically, our results showed that deficiency of IKKε might inhibit the phosphorylation of IκBα, p65, RelB ,and p100 in mouse heart tissues after Dox stimulation. In summary, our research suggests that IKKε might play an essential role in the development of Dox-induced dilated cardiomyopathy and maybe a potential target for the treatment of dilated cardiomyopathy in the future.
Efficient Security Sharing Scheme Using Block chain in Internet of Things
Authors: C Sathish , C Rubavathi Yesubai 
Published: June 2022

Abstract
Recently, the organizations and different institutions are utilized cloud service providers to save the information for sharing information with the consideration of Internet of Things (IoT). In this storage, third party access is a biggest issue for reducing unwanted access and data theft. So, this paper, Block chain based Security Sharing scheme with Data Access Control (BSSDAC) is developed for empowering the access control and secures operation of data transaction. The major main of this research towards empower the security and data access control in the IoT applications. Cipher text-Policy Attribute-Based Encryption (CP-ABE) can be developed to enhance the security of personal data. In the CP-ABE, the key generation is achieved by Aquila Optimization Algorithm (AOA). To maintain the owner's security, the Block chain based Data Access Control (DAC) can be designed. To maintain secure data storage in addition sharing, the Block chain based Cipher text-Policy Attribute-Based Encryption (CP-ABE) is designed. The data owner is empowering the security and data access control with the consideration of Block chain technology. Finally, the security of the data, access control of the data owner can be achieved through the Block chain based approach. The proposed method is implemented in MATLAB and performances are evaluated. The proposed technique is contrasted with the existing technologies such as Elliptic Curve Cryptography (ECC) in addition Rivest–Shamir–Adleman (RSA) respectively.
A smart algorithm to optimally manage the charging strategy of the Home to Vehicle (H2V) and Vehicle to Home (V2H) technologies in an off-grid home powered by renewable sources
Authors: MAZZEO Domenico , Matera Nicoletta , De Luca Rocco , Musmanno Roberto 
Published: June 2022

Abstract
European directives promote activities aimed at developing a sustainable and decarbonised energy system, with a greater share of electricity production from renewable sources, within a framework of greater efficiency. In the energy of nZEB buildings, the reduction of energy needs is accompanied by the use of hybrid renewable energy systems (HRES) for the production of energy on-site to be used for appliances and lighting and also for mobility through the electric vehicle (EV) charging. With reference to this framework, the paper studied the integration of Vehicle to Home (V2H) technology in HRES for residential users. The HRES consists of a photovoltaic (PV) generator, a wind generator, an electrical storage system and a charging station for an EV, in a stand-alone context. V2H technology provides for the use of the EV as an emergency energy source if the generation and storage systems are unable to guarantee the satisfaction of the residential load. For this purpose, an algorithm is proposed that can manage and optimize the dissipated energy produced in excess, providing that this is sent, when possible, to the EV for extra charging compared to the residential charging expected in the load determination. This extra charging process does not affect the load required by the residential user being carried out directly by the generation system with the excess energy produced. With reference to an isolated single-family user not connected to the network and to the use of an EV (which ensures mobility), two energy scenarios were considered, in the absence and the presence of V2H technology. The possibility of EV charging at night and an additional extra charge during the day were considered. The load required for nocturnal EV charging rests on the residential load of the home, while the extra charge is directly borne by the generation system. To reduce excess energy, which in the considered context must be dissipated, two charging strategies were considered that differ in the choice of whether or not to carry out nocturnal charging depending on the EV state of charge. The different system configurations and use of the generated energy make the energy analysis complete to identify the performance of these systems. The dynamic simulations of the HRES operation were carried out through TRNSYS, for the dynamic simulation of the PV and wind systems, which is coupled with MATLAB to dynamically simulate the residential battery and the H2V and V2H technologies. Starting from the hourly results obtained, an yearly energy analysis was carried out.
Down-regulation of hepatic CLOCK by PPARα is involved in inhibition of NAFLD
Authors: Luo Jia , Yan Zheng , Xu Liping , Zhang Haoyue , Xi Yang , Yang Julin , Liu Aiming 
Published: June 2022

Abstract
This work aimed to investigate the role of nuclear factor peroxisome proliferator-activated receptor α (PPARα) in modification of circadian clock and their relevance to development of nonalcoholic fatty liver disease (NAFLD). Both male wild-type (WT) and Pparα-null (KO) mice treated with high-fat diet (HFD) were used to explore the effect of PPARα and lipid diet on the circadian clock. PPARα humanized (hPPARα) mice were treated with PPARα agonist fenofibrate to reveal the hPPARα-dependence of circadian locomotor output cycles kaput (CLOCK) down-regulation. Hepatocytes were challenged with oleic acid and WY-14643 to verify the action of PPARα in down-regulating CLOCK and lipid accumulation. Strongest NAFLD developed in mice fed 45%HFD and it was inhibited in WT mice. The night/day diet consumption ratio was inhibited more in WT mice than that in KO mice on HFD. The core circadian factor CLOCK was down-regulated by HFD in both WT and KO mice in the liver, not in the hypothalamus. More interestingly, hepatic CLOCK was down-regulated by basal PPARα and activated hPPARα in dose-dependence of fenofibrate. According with the in vivo experiments, CLOCK down-regulation by activated PPARα was involved in inhibition of lipid accumulation in hepatocytes. Down-regulation of hepatic CLOCK by basal PPARα contributes to tolerance against development of NAFLD. Inhibition of CLOCK by activated PPARα is involved in inhibition of NAFLD by PPARα agonists.