bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: High-throughput preclinical screening of CD123 scFv to design 3rd generation of CAR T-cell in the treatment of BPDCN patient
Authors: Fredon Maxime , Poussard Margaux , Biichle Sabeha , Bonnefoy Francis , Seffar Evan , Bôle-Richard Elodie , Renosi Florian , Boidot Romain , Anna François , Loustau Maria , Caumartin Julien , Saas Philippe , Deconinck Eric , Daguindeau Etienne , Roussel Xavier , Adotevi Olivier , Angelot-Delettre Fanny , Galaine Jeanne , Garnache-Ottou Francne 
Published: May 2022

Abstract
Background: Blastic Plasmacytoid Dendritic Cell Neoplasm (BPDCN) is an aggressive malignant hemopathy characterized by constant overexpression of the CD123 antigen on 100% of blasts identifying CD123 as an antigenic target for the development of adoptive cell therapy. Chimeric Antigen Receptor-T (CAR-T) are composed of an extracellular domain consisting of a single-chain fragment variable (scFv) targeting a surface antigen expressed on tumor cells. In view of the low expression of CD123 on hematopoietic stem cells, monocytes and endothelial cells, it is necessary to evaluate and select the CD123 CAR-T allowing the best cytotoxicity differential between leukemic and healthy cells. Methods: We developed five third-generation CD123 CAR-T by substituting scFv, in order to assess changes in efficacy and on-target/off-tumor side effects. Using various in vitro and in vivo BPDCN models, we evaluated the functionality on BPDCN and the on-target/off-tumor effect on endothelial cell line, monocytes and hematopoietic stem cell (HSC). Results: Using Incucyte, we confirmed the low cytotoxicity on endothelial cells. We showed an increase in CD123 expression on endothelial cells, dependent on the activation of CAR-T through cytokine secretion. Evaluated by CFU-GM culture, we show that two CAR-T are less cytotoxic against hematopoietic stem cells and these two CAR-T significantly reduce tumor infiltration and increase overall survival of mice in three in vivo BPDCN models. Finally, we demonstrate in bulk RNA-sequencing that the most effective CAR-T in an aggressive BPDCN model, upregulates genes associated with cytotoxicity and activation/exhaustion at day 34, with fewer regulatory signaturesConclusions: Together, these results emphasize the importance of evaluating different scFv for the development of CAR-T, to select the best one, with high cytotoxicity potential and the best safety profile for clinical development.
Bioinformatic Analyses of Potential Key Genes in the Pathogenesis of Idiopathic Pulmonary Fibrosis
Authors: Wang Peijie , Shu Wanhong , Yin Fei , Zhang Qiwei , Yin Yin , Deng Qiying , Zeng Feifei , Li Yun , Zhang Junhang 
Published: May 2022

Abstract
Purpose: Idiopathic pulmonary fibrosis (IPF) is an advancing, chronic and fatal interstitial lung disease with a poor prognosis and increasing incidence. Our study aimed to learn the molecular mechanisms of IPF progression to gain a better comprehension of IPF at the biological level.Methods: In our study, we used the online Gene Expression Omnibus (GEO) microarray expression profiling dataset GSE15197 and GSE48149 to identify differentially expressed genes (DEGs) between IPF patients and normal controls. Then, tissue-specific gene expression, functional enrichment, and protein-protein interaction (PPI) network analyses were performed.Results: In total, 183 DEGs were identified in the differential expression analysis, including 56 downregulated genes and 127 upregulated genes. The PPI network analysis and manual screening of tissue-specific gene expression resulted in the identification of 51 tissue-specific expressed DEGs, among which 25 were tissue-specifically expressed in the respiratory system. In addition, functional enrichment and biological process analysis identified 7 upregulated genes (SERPINB5, COL17A1, CLCA2, KRT17, KRT5, CDH3) and 6 downregulated genes (CLDN18, CRTAC1, AGER, SFTPA2, SFTPA1, ABCA3) specifically expressed in the respiratory system and indicated that the processes of extracellular matrix organization, protein digestion, and absorption signaling pathway and hemidesmosome assembly played main roles in the pathogenesis of IPF.Conclusions: This bioinformatic analysis may provide useful information for elucidating novel mechanisms underlying IPF. 13 main DEGs specifically expressed in the respiratory system, which are involved in processes of hemidesmosome assembly, cell-substrate junction assembly, and cell junction assembly, may play main roles in the pathogenesis of progression of IPF.
Low Temperature Plasma Suppresses Proliferation, Invasion, Migration and Survival of SK-BR-3 Breast Cancer Cells
Authors: Li Xiang , Sun Tao , Zhang Xinru , Hou Chao , Shen QiYing , Wang Dong , Ni Guohua 
Published: May 2022

Abstract
Low temperature plasma (LTP) is a developing field in recent years to play important roles of sterilization, material modification and wound healing. Breast cancer is a common gynecological malignant tumor. Recent studies have shown that LTP is a promising selective anti-cancer treatment, because some cancer cells are highly sensitive to reactive oxygen species (ROS) produced by LTP, but non-tumor cells are highly resistant to it, which indicates that the main effect of LTP is mediated through the generation of ROS. The effect of LTP on breast cancer is still unclear. In this study, We treated breast cancer cell lines with low temperature plasma for different periods of time and analyzed the relevant differences. we found that LTP was able to suppress cell vitality, proliferation, invasion and migration of SK-BR-3 cells. Also, SK-BR-3 apoptosis was induced by LTP in a time-dependent manner. These evidences suggest the negative effect of LTP on malignant development of SK-BR-3 cells, and LTP has the potential clinical application for breast cancer treatment.
Room temperature synthesis of water-soluble spherical particles of a uniform diameter composed of carbon nanobelts and C60 molecules
Authors: Choi Sieun , Kurosu Shunji , Marieshiko Yuta , Maekawainakawa Takanobu , Maekawa Toru 
Published: May 2022

Abstract
A carbon nanobelt (CNB) is a loop of fused benzene rings and in a sense a cutout of a single-walled carbon nanotube. Various types of CNBs have been successfully synthesized in recent years and CNBs have been utilized for the development of practical devices. In this study, we investigate secondary structures formed by (6,6)CNBs and C60 molecules, which are dissolved in 1,2-dichlorobenzene. We find that uniform spherical particles are formed by (6,6)CNBs and C60 molecules in 1,2-dichlorobenzene at room temperature via bottom-up self-assembly, setting the molar concentrations of (6,6)CNBs and C60 molecules at appropriate values, and furthermore those particles are monodisperse even in water. The present facile room temperature synthetic methodology can be applied to the creation of nano/micro structures/materials using basic carbon nano units such as cycloparaphenylene (CPP, carbon nanorings) and fullerenes; e.g., C60, C70 and C59N.
Validation of the Death Literacy Index and benchmarking of death literacy level in a representative UK population sample
Authors: Graham-Wisener Lisa , Toner Paul , Leonard Rosemary , Groarke Jenny M. 
Published: May 2022

Abstract
BackgroundDeath literacy includes the knowledge and skills that people need to gain access to, understand, and make informed choices about end of life and death care options. The Death Literacy Index (DLI) can be used to determine levels of death literacy across multiple contexts, including at a community/national level, and to evaluate the outcome of public health interventions. As the first measure of death literacy, the DLI has potential to significantly advance public health approaches to palliative care. The current study aimed to provide the first assessment of the psychometric properties of the DLI in the UK, alongside population-level benchmarks. MethodsA large nationally representative sample of 399 participants, stratified by age, gender and ethnicity, were prospectively recruited via an online panel. The factor structure of the 29-item DLI was investigated using confirmatory factor analysis. Internal consistency of subscales was assessed alongside interpretability. Hypothesised associations with theoretically related/unrelated constructs were examined to assess convergent and discriminant validity. Descriptive statistics were used to provide scaled mean scores on the DLI.ResultsConfirmatory factor analysis supported the original higher-order 8 factor structure, with the best fitting model including one substituted item developed specifically for UK respondents. The subscales reported high internal consistency. Good convergent and discriminant validity was evidenced in relation to objective knowledge of the death system, death competency, actions relating to death and dying in the community and loneliness. Good known-groups validity was achieved with respondents with professional/lived experience of end-of-life care reporting higher levels of death literacy. There was little socio-demographic variability in DLI scores. Scaled population-level mean scores were near the mid-point of DLI subscale/total, with comparatively high levels of experiential knowledge and the ability to talk about death and dying. ConclusionsPsychometric evaluations suggest the DLI is a reliable and valid measure of death literacy for use in the UK, with population level benchmarks suggesting the UK population could strengthen capacity in factual knowledge and accessing help. International validation of the DLI represents a significant advancement in outcome measurement for public health approaches to palliative care. Pre-registrationhttps://osf.io/fwxkh/
Indicators of climate change and violence against women predict estimated prevalence of modern slavery: An Ecofeminist Perspective
Authors: Cameron Erinn , Trend-Cunningham Fiona , Ray Janine M. , Jacquin Kristine M. 
Published: May 2022

Abstract
Climate change, violence against women (VAW), and modern slavery are significant human rights crises to which women are disproportionately vulnerable. Our research examined the complex relationship between indicators of climate change, VAW, and the estimated prevalence of modern slavery across 180 countries. Regression analysis revealed significant results suggesting that countries where climate change and VAW are prevalent may support conditions that exacerbate vulnerability to exploitation. Results are placed within a gender framework, highlighting ecofeminism theory for an examination of patriarchal and capitalistic systems and their relationship to the oppression of women, the degradation of the environment, and modern slavery.
Chemical constituents from the rhizome of Dioscorea bulbifera L.
Authors: Zhang Yunqiang , Han Tingting , Wang Hongpeng , Li Meichen , Xia Feiruo , Liu Jianyu , Xu Yongnan 
Published: May 2022

Abstract
A phytochemical investigation of the rhizome of Dioscorea bulbifera L. led to the isolation of twenty compounds, including eight phenanthrenes (1–8), eight flavonoids (9-16), two diterpenes (17, 18), one bibenzyl derivatives (19), and one acid (20). The structures of these compounds were elucidated on the basis of spectroscopic analysis and comparison with previous literature data. Among them, 1 and 16 were isolated from the rhizome of Dioscorea bulbifera L. for the first time and compounds 16 first reported from the genus Dioscorea. They were tested in vitro for cytotoxic activities against CT26 cell lines and Compound 1 exhibits cytotoxic activity with IC50 value of 83.23 μM.
Cyberbullying among Adolescents in Saint Vincent and the Grenadines: Psychosocial Correlates for School-Based Health Promotion Interventions
Authors: Mireku Dickson Okoree , Sarfo Jacob Owusu , Seidu Abdul-Aziz , Ahinkorah Bright Opoku 
Published: May 2022

Abstract
Background This study aimed to investigate the prevalence and psychosocial correlates of cyberbullying among in-school adolescents in Saint Vincent and the Grenadines. MethodWe explored the 2018 Saint Vincent and the Grenadines’ Global School-based Health Survey with a sample size of 1,877 in-school teenagers (aged 11 - 18 years old). Using a binomial logistic regression modelling, statistically estimated Adjusted Odds Ratios (AOR) at a 95% confidence interval were utilised to determine the predictors of cyberbullying. ResultsResults showed a 15.7% (10.8% males vs. 20.5% females) prevalence of cyberbullying among in-school teenagers. Also, psychosocial correlates like being a female (AOR 2.387, 95% CI 1.694 – 3.362), being physically attacked (AOR 0.702, 95% CI 0.494 –0.997), being involved in a physical fight (AOR .702, 95% CI 0.499 – 0.988), being bullied on a school property (AOR 0.458, 95% CI 0.326 – 0.645), being bullied off a school property (AOR 0.261, 95% CI 0.185 – 0.368), having ever considered suicide (AOR 0.577, 95% CI 0.418–0.796), and having ever had sex (AOR 0.484, 95% CI 0.350–0.669) significantly predicted cyberbullying. ConclusionFurther, school-based health interventions focusing on the identified psychosocial correlates are required to mitigate cyberbullying in Saint Vincent and the Grenadines.Trail Registration: Global School-Based Student Health Survey 2018 (VCT_2018_GSHS_v01) Registered August 20, 2021, https://extranet.who.int/ncdsmicrodata/index.php/catalog/878.
Can mobile-bearing unicompartmental knee arthroplasty achieve natural gap-balancing： an observational study with novel pressure sensor
Authors: Nan Shaokui , Cao Zheng , Song Yue , Kong Xiangpeng , Li Haifeng , Chai Wei 
Published: May 2022

Abstract
BackgroundMobile-bearing unicompartmental knee arthroplasty (MB-UKA) is an effective treatment for anteromedial knee osteoarthritis. Meticulous intraoperative soft tissue balancing remains challenging yet consequential for a successful operation. Currently, surgeons rely mostly on their experience during soft tissue balancing, yielding unreproducible results. This study aimed to further optimize clinical results of MB-UKA through quantitative measurements of intraoperative medial compartment soft tissue tension and determination of target tension values most consistent with the natural state.MethodsThis was an observational study of 24 consecutive patients. All 30 UKAs were performed by a single surgeon. The piezoresistive sensor was custom designed to fit in the medial compartment gap. Contact pressures were measured at 5 angular positions of the knee intraoperatively: 0°, 20°, 45°, 90°, and 110° of flexion. The change in pressure from extension (20° position) and flexion (110° position) was also calculated (E-FPD). Data on age, sex, body mass index, operative side, and bearing size were collected. Outcome measures were measured at baseline and at the 6-month postoperative follow-up; Oxford Knee Score, visual analog scale score, and range of motion were compared to evaluate clinical outcomes. Results There was a significant improvement in patients in all measured outcomes at 6 months from baseline. The E-FPD of 14.9 N (8.9, 24.6) was indicative of appropriate soft tissue balancing throughout the functional range of knee motion. Of 30 knees, 22 were 3-mm bearing and 8 were 4- or 5-mm bearing. The pressure data of the 3-mm bearing group was larger than that of the non-3-mm bearing group for each knee flexion degree, but the difference was not statistically significant.Conclusions Objective data from sensor output may assist surgeons in decreasing loading variability during MB-UKA. The data suggested that MB-UKA could not accurately restore soft tissue tension to the natural state, which was related to the inability of MB-UKA surgical instruments to fine adjust the bone cut and soft tissue release. We suggest designing a new type of 3.5 G-clamp to help better obtain the soft tissue tension close to the natural tension.Study registration:Chinese Clinical Trial Registry (http://www.chictr.org.cn): ChiCTR1900024146.
Bacterial infection is a risk factor for progression to acute-on-chronic liver failure in patients with severe hepatitis flare of HBV-related compensated liver cirrhosis
Authors: Lin Shide 
Published: May 2022

Abstract
Aims
The aims of this study were to investigate the risk factors for bacterial infection (BI) and the association of BI with progression to acute-on-chronic liver failure (ACLF) in patients with severe hepatitis flare of hepatitis B virus (HBV)-related compensated liver cirrhosis.
Methods
237 patients with HBV-related compensated liver cirrhosis and severe hepatitis flare were retrospectively reviewed. Baseline demographics characteristics, biochemical were compared between patients with and without occurring of BIs and progression to ACLF. Univariate and multivariate logistic regression were used to identify independent risk factors associated with development of BI and ACLF.
Results
48 (20.3%) patients progressed to ACLF after admission. 101 (42.6%) patients progressed to hepatic decompensation (HD) and 52 (20.3%) patients had BIs before development of ACLF. Patients with BIs had significantly higher incidence of HD (73.1%) and ACLF (46.2%) than those without BIs (34.1% and 13.0%, respectively, P < 0.01). Total bilirubin (TBil, OR = 1.003, 95% CI: 1.000-1.006) and Child-turcotte-pugh (CTP) score (OR = 1.745, 95% CI: 1.345–2.265) were identified as independent risk factors associated with BI. BI (OR = 7.113, 95% CI: 2.714–18.644), gamma-glutamyl transpeptidase (OR = 0.094, 95% CI: 0.988–0.999), TBil (OR = 1.004, 95% CI: 1.001–1.007), international normalized ratio (OR = 114.05, 95% CI:17.4-746.3) and platelet (OR = 0.984, 95% CI: 0.972–0.996) were independent risk factors associated with progression to ACLF.
Conclusion
High level of TBil and CTP score are risk factors associated with occurring of BI and BI is a risk factor related with progression to ACLF in patients with HBV-related compensated liver cirrhosis and severe hepatitis flare.
A Systematic Review on Evolving the Bowel Cancer Screening Programme in England: Targeted Screening for Individuals with First-Degree Family History of Bowel Cancer
Authors: Chigonda Rutendo Lisa 
Published: May 2022

Abstract
First-degree relatives of bowel cancer cases have an approximately twofold risk of the disease compared with those without a family history. The more affected first-degree relatives an individual has the higher the absolute and relative risk for developing bowel cancer. At present, widespread screening of average-risk individuals for bowel cancer occurs in England, however, the Bowel Cancer Screening Programme does not currently provide a framework for the identification of a family history of bowel cancer. The aim is to determine the cost effectiveness and benefits of offering targeted screening to individuals with a first-degree family history of bowel cancer. The primary outcomes examined the prevalence of developing bowel cancer and estimated the cost effectiveness to inform screening policy decisions. The secondary outcome evaluated the possible screening barriers in the adoption of bowel screening of first-degree relatives. Data presented suggests that screening first-degree relatives of patients with bowel cancer is likely to be cost effective to the NHS, and that compared with the average-risk group using colonoscopy, advanced neoplasia was significantly more prevalent in those with first-degree relatives with bowel cancer. Targeted screening would mean less long-term health costs however, given the limited resources on NHS cost analysis data, cost savings associated with targeted screening are largely unknown; although data presented from European studies strongly suggests that it may be optimistic. Opportunities for this review include its recommendation to modify the screening criteria to extend to first-degree relatives of bowel cancer patients and for the National Screening Committee to implement this protocol nationwide.
Vascular Density Changes in Retina and Optic Nerve in OSAS Patients Using and Not Using CPAP
Authors: Oba Turker , Arman Berkay Hasan , Cicek Feyza , Onder Feyza 
Published: May 2022

Abstract
Background To compare optic disc and macular vascular density (VD) between the control group and severe obstructive sleep apnea syndrome (OSAS) patients with using CPAP or not.Methods Evaluation was made of 60 eyes of patients with severe OSAS using CPAP, 60 eyes of patients with severe OSAS not using CPAP, and 30 control groups, using OCTA RT XR Avanti (AngioVue software, Optovue Inc., Fremont, CA). Optic nerve head (ONH) and macular VD were measured in 4 quadrants. Retinal nerve fiber layer (RNFL) thickness and ganglion cell complex (mGCC) thickness were evaluated with Spectral Domain OCT for all patients. Results In OSAS patients using CPAP, there was no significant thinning in the RNFL and mGCC thickness compared to the control group (p>0.05). Peripapillary VD was significantly different in superior-inferior-nasal quadrants in patients using CPAP (p<0.001, p=0.001, p=0.004, respectively) and was affected in the nasal quadrant in patients not using CPAP when compared with the control group (p=0.012). In eyes with no use of CPAP, macular superficial and perifoveal deep vascular density differed from control in all sectors (all, p<0.05), while only deep VD was reduced in patients using CPAP (p=0.005, p>0.05, respectively).Conclusion Although OSAS causes a significant decrease in macular vessel density, it does not cause significant damage to structural elements such as RNFL and mGCC. While the use of CPAP has a positive effect by providing oxygenation to the macular superficial perfusion, it significantly reduces the peripapillary perfusion by causing an increase in IOP. Severe cases using CPAP may have a significant risk for glaucoma. Therefore, OCTA may be an ideal method to evaluate the changes in the retinal vascular system, which could be an effective parameter for the early evaluation of severe OSAS patients.
Activation of LRP1 ameliorates cerebral ischemia/reperfusion injury and cognitive decline by suppressing neuroinflammation and oxidative stress through TXNIP/NLRP3 signaling pathway in mice
Authors: Yang Chengjie , Chen Ye , Li Xin , Feng Xiaoqing , Feng Jianguo , Jia Jing , Wei Jicheng , Zhou Jun 
Published: May 2022

Abstract
Cerebral ischemia/reperfusion (I/R) injury is a clinical event associated with high morbidity and mortality. Neuroinflammation plays a crucial role in the pathogenesis of I/R-induced brain injury and cognitive decline. Low-density lipoprotein receptor-related protein-1 (LRP1) can exert strong neuroprotection in experimental intracerebral hemorrhage. However, whether LRP1 can confer neuroprotective effects after cerebral I/R is yet to be elucidated. The present study aimed to investigate the effects of LRP1 activation on cerebral I/R injury and deduce the underlying mechanism involving TXNIP/NLRP3 signaling pathway. Cerebral I/R injury was induced in mice by bilateral common carotid arteries occlusion. LPR1 ligand, apoE-mimic peptide COG1410 was administered intraperitoneally. To elucidate the underlying mechanism, overexpression of TXNIP was achieved via the hippocampal injection of AAV-TXNIP before COG1410 treatment. Neurobehavioral tests, brain water content, immunofluorescence, western blot, enzyme-linked immunosorbent assay, HE and terminal deoxynucleotidyl transferase dUTP nick end labeling staining were performed. Our results showed that the expressions of endogenous LRP1, TXNIP, NLRP3, and cleaved caspase-1 were increased after cerebral I/R. COG1410 significantly ameliorated cerebral I/R-induced neurobehavioral deficits, brain edema, histopathological damage, and poor survival rate. Interestingly, COG1410 inhibited microglia pro-infammatory polarization and promoted anti-infammatory polarization, decreased oxidative stress, attenuated apoptosis, and inhibited the expression of the TXNIP/NLRP3 signaling pathway. However, the benefits of COG1410 were abolished by TXNIP overexpression. Thus, our study suggested that LRP1 activation with COG1410 attenuated cerebral I/R injury at least partially related to modulating microglial polarization through TXNIP/NLRP3 signaling pathway in mice. Thus, COG1410 treatment might serve as a promising therapeutic approach in the management of cerebral I/R patients.