bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endothelial-derived sphingolipids are required for vascular development and systemic lipid homeostasis
Authors: Kuo Andrew , Checa Antonio , Niaudet Colin , Jung Bongnam , Fu Zhongjie , Wheelock Craig E. , Singh Sasha A. , Aikawa Masanori , Smith Lois E. , Proia Richard L. , Hla Timothy 
Published: March 2022

AbstractSerine palmitoyl transferase (SPT), the rate-limiting enzyme in the de novo synthesis of sphingolipids (SL), is needed for embryonic development, physiological homeostasis, and response to stress. The functions of de novo SL synthesis in vascular endothelial cells (EC), which line the entire circulatory system, are not well understood. Here we show that the EC de novo synthesis not only impacts vascular development but also maintains normal SL metabolic homeostasis in the circulatory system and peripheral organs. Mice with an endothelial-specific gene knockout of SPTLC1 (Sptlc1 ECKO), an essential subunit of the SPT complex, exhibited EC-intrinsic effects including reduced EC proliferation and tip/stalk cell differentiation, resulting in delayed retinal vascular development. In addition, Sptlc1 ECKO mice had reduced pathological retinal neovascularization in the oxygen-induced retinopathy model, suggesting that EC SL produced from the de novo pathway are needed for efficient VEGF signaling within the vascular system. Post-natal deletion of the EC Sptlc1also showed cell-extrinsic effects, including rapid reduction of several SL metabolites in plasma, red blood cells and peripheral organs (lung and liver) but not in the retina, part of the central nervous system (CNS). In the liver, EC de novo SL synthesis was required for acetaminophen-induced ceramide elevation and hepatotoxicity. These results suggest that EC-derived SL metabolites are in constant flux between the vasculature, circulatory elements, and parenchymal cells of non-CNS organs. Taken together, our data point to the central role of the endothelial SL biosynthesis in maintaining vascular development and neovascular proliferation, non-CNS tissue metabolic homeostasis and hepatocyte response to stress.
Small Molecule Modulation of the Archetypal UbiB protein COQ8
Authors: Murray Nathan H. , Lewis Adam , Asquith Christopher R. M. , Rincon Pabon Juan P. , Fang Zixiang , Ptak Naomi , Smith Robert W. , Vasta James D. , Zimprich Chad A. , Corona Cesear R. , Robers Matthew B. , Bingman Craig A. , Gross Michael L. , Henzler-Wildman Katherine , Pagliarini David J. 
Published: March 2022

AbstractSmall molecule tools have enabled mechanistic investigations and therapeutic targeting of the protein kinase-like (PKL) superfamily. However, such tools are still lacking for many PKL members, including the highly conserved and disease-related UbiB family. Here, we sought to develop and characterize inhibitor and activator molecules for the archetypal UbiB member, COQ8, whose function is essential for coenzyme Q (CoQ) biosynthesis. Guided by crystallography, activity assays, and cellular CoQ measurements, we repurposed the 4-anilinoquinoline scaffold to selectively inhibit human COQ8A in cells. Second, using 1H-13C HMQC NMR and hydrogen-deuterium exchange mass spectrometry, we reveal that the CoQ precursor mimetic, 2-propylphenol (2-PP), modulates the quintessential UbiB KxGQ domain to increase COQ8A nucleotide affinity and ATPase activity. Our newfound chemical tools promise to lend new mechanistic insights into the activities of these widespread and understudied proteins and to offer potential therapeutic strategies for human diseases connected to their dysfunction.
Cellulosic copper nanoparticles and a hydrogen peroxide-based disinfectant protect Vero E6 cells against infection by viral pseudotyped particles expressing SARS-CoV-2, SARS-CoV or MERS-CoV Spike protein
Authors: Brault Ariane , Néré Raphael , Prados Jérôme , Boudreault Simon , Bisaillon Martin , Marchand Patrick , Couture Patrick , Labbé Simon 
Published: March 2022

AbstractSevere acute respiratory syndrome (SARS) is a viral respiratory infection caused by human coronaviruses (HuCoV) that include SARS-CoV-2, SARS-CoV, and Middle East respiratory syndrome coronavirus (MERS-CoV). Although their primary mode of transmission is through contaminated respiratory droplets from infected carriers, the deposition of expelled virus particles onto surface and fomites could contribute to viral transmission. Here, we use replication-deficient murine leukemia virus (MLV) pseudoviral particles expressing SARS-CoV-2, SARS-CoV, or MERS-CoV Spike (S) protein on their surface. These surrogates of native coronavirus counterparts serve as a model to analyze the S-mediated entry into target cells. Carboxymethyl cellulose (CMC) nanofibers that are combined with copper (Cu) exhibit strong antimicrobial properties. S-pseudovirions that are exposed to CMC-Cu nanoparticles (30 s) display a dramatic reduction in their ability to infect target Vero E6 cells, with ∼97% less infectivity as compared to untreated pseudovirions. In contrast, addition of the Cu chelator tetrathiomolybdate protects S- pseudovirions from CMC-Cu-mediated inactivation. When S-pseudovirions were treated with a hydrogen peroxide-based disinfectant (denoted SaberTM) used at 1:16 dilution, their infectivity was dramatically reduced by ∼98%. However, the combined use of SaberTM and CMC-Cu is the most effective approach to restrict infectivity of SARS-CoV-2-S, SARS-CoV-S, and MERS-CoV-S pseudovirions in Vero E6 cell assays. Together, these results show that cellulosic Cu nanoparticles enhance the effectiveness of diluted SaberTM sanitizer, setting up an improved strategy to lower the risk of surface- and fomite-mediated transmission of enveloped respiratory viruses.
Hand cold pressor test induces thermogenesis in upper thoracic regions as measured by skin surface infrared thermography
Authors: Levtova Natalie , Benoit Emilie-Anne , Jhajj Aneet K. , Narayana Thivaedee , Purkayastha Dipannita , Salimi Ali , Kakon Gabriel , Maurice-Ventouris Meagane E. I. , Kaffash Mohamadi Amir Arshiya , Kizhner Tomas , Khayargoli Pranamika , Darlington Peter J. 
Published: March 2022

Background
Cold exposure may cause health problems and impaired productivity in outdoor or cold-temperature workers. The cold pressor test (CPT) is a laboratory procedure that measures cardiovascular and thermoregulatory responses to acute cold exposure such as metabolic activity in brown adipose tissue. How the body responds to acute cold exposure of a hand is not completely understood. We tested the hypothesis that the upper thorax produces heat during a single hand-CPT, which restores warmth to the cold-expose appendage.
Objectives
The objective was to measure skin temperature changes in the upper thoracic regions and the cold-exposed appendage during a CPT. The secondary objective was to determine if cardiovascular or psychological responses during CPT accounted for skin temperature changes.
Methods
50 healthy participants immersed their right hand up to wrist level in 4 °C water for three minutes. Surface skin temperatures were imaged by infrared thermography at baseline, during CPT, and in the recovery phase. Sublingual oral temperature and water bath temperature were recorded throughout the test. Cardiovascular responses were monitored by continuous finger pulse-wave plethysmography. Peak pain and peak stress were reported by the participants on a Likert scale.
Results
CPT increased the systolic blood pressure (+22 mmHg, p < 0.001), diastolic blood pressure (+15 mmHg, p < 0.001) and heart rate (+7 beats per minute, p = 0.024). During CPT, skin temperature increased on thoracic regions including mediastinal (+0.5°C, p < 0.021), sternal (+0.5°C, p < 0.002), right supraclavicular (+0.3°C, p < 0.042) and left supraclavicular (+0.3°C, p < 0.016) regions. During CPT, the hand was cooler on ventral (-14.6°C p < 0.001) and dorsal (-15.2°C p < 0.001) sides, and warmed up during recovery. The ventral forearm, dorsal forearm, antecubital fossa, and adjacent medial epicondyle region were significantly cooler throughout the recovery time. The oral temperature did not change during CPT. There were no correlations between the change in mediastinal skin temperature and the sex of the participant, or changes in cardiovascular parameters, peak pain, or peak stress values.
Conclusions
Localized hand cooling caused a rapid warming of the thorax, dissipation of cold in the forearm, and rewarming of the hand during recovery. Thermoregulation was not dependant on pain, stress, sex, or cardiovascular changes between participants. By understanding thermoregulation, better approaches can be developed to mitigate the negative impacts of cold exposure.
A Single Cell Cloning Platform for Gene Edited Functional Murine Hematopoietic Stem Cells
Authors: Becker Hans Jiro , Ishida Reiko , Wilkinson Adam C. , Kimura Takaharu , Jann Lee Michelle Sue , Coban Cevayir , Ota Yasunori , Tojo Arinobu , Kent David , Yamazaki Satoshi 
Published: March 2022

AbstractGene editing using engineered nucleases frequently produces on- and off-target indels in hematopoietic stem cells (HSCs). Gene-edited HSC cultures thus contain genetically heterogenous populations, the majority of which either do not carry the desired edit or harbor unwanted mutations. In consequence, transplanting edited HSCs carries the risks of suboptimal efficiency and of unwanted mutations in the graft. Here, we present an approach for expanding gene-edited HSCs at clonal density, allowing for genetic profiling of individual clones before transplantation. We achieved this by developing a defined, polymer-based expansion system and identifying long-term expanding clones within the CD201+CD150+CD48-c-Kit+Sca-1+Lin- population of pre-cultured HSCs. Using the Prkdcscid immunodeficiency model, we demonstrate that we can expand and profile edited HSC clones to check for desired and unintended modifications. Transplantation of Prkdc-corrected HSCs rescued the immunodeficient phenotype. Our ex vivo-manipulation platform establishes a novel paradigm to control genetic heterogeneity in HSC gene editing and therapy.
Effect of rare earth on microstructure and corrosion behavior of electroless Ni-W-P composite coatings
Authors: Jia Ya-Peng , Sun Wan-Chang , Xiao Yan , Liu Jing-Pei , Zhang Cong-Xiao , Zhang Tong-Qiang , Hou Ze-Feng 
Published: March 2022

Purpose
This paper aims to research the effect of different concentrations for Nd(NO3)3 and Ce(NO3)3 on the microstructures and corrosion resistance of Ni-W-P composite coatings through electroless plating method.

Design/methodology/approach
Scanning electron microscope, attached energy dispersive spectroscopy system and X-ray diffraction were used in this work. Meanwhile, the immersion test and electrochemical tests were used to characterize the corrosion behavior of the coating.

Findings
The coatings prepared at 1.00 g·L−1 Nd(NO3)3 exhibit a dense structure and high phosphorus content (12.38 wt.%). In addition, compared to the addition of Ce(NO3)3, when Nd(NO3)3 was introduced at a concentration of 1.00 g·L−1, the minimum corrosion rate of the coating was 1.209 g·m−2·h−1, with a noble Ecorr (−0.29 V) and lower Icorr (8.29 × 10−4 A·cm−2).

Originality/value
The effects of rare earths on the deposition and corrosion resistance mechanisms of Ni-W-P composite coatings were explored, with the rare earth elements promoting the deposition of nickel and tungsten atoms. Simultaneously, the amorphization of the coating increases, which excellently enhances the corrosion resistance of the coating.
A new approach for optimal photovoltaic resources in distribution networks with reconfiguration based on L_∞
Authors: Badr Hussein Mohammed , Ali Ramzy Salim , Mahmood Jawad Radhi 
Published: March 2022

Purpose
In the vast majority of published papers, the optimal allocation of photovoltaic distributed generation (PVDG) units and reconfiguration problems are proposed along with the number of PVDG used in the simulation. However, optimisation without selecting the number of PVDG units installed in the distribution grid is insufficient to achieve a better operational performance of power systems. Moreover, multi-objective installation of PVDG units and reconfiguration aims to simultaneously relieve congestion problems, improve voltage profile and minimise the active and reactive power losses. Therefore, this paper aims to propose a new modified camel algorithm (NMCA) to solve multi-objective problems considering radial distribution system to achieve secure and stable operation of electric power system with good performance.

Design/methodology/approach
In this paper, the decision variables include the location and size of PVDG units with specific rang to determine the number of PVDG units needed to install and open network lines determined using NMCA based on the L_∞ technique. This also satisfies the operating and radial constraints. Furthermore, a benchmark comparison with different well known optimisation algorithms has been made to confirm the solutions. Finally, an analysis of the findings was conducted, and the feasibility of solutions was fully verified and discussed.

Findings
Two test systems – the institute of electrical and electronics engineers (IEEE) 33-bus and IEEE 69-bus, were used to examine the accuracy and effectiveness of the proposed algorithm. The findings obtained amply proved the efficiency and superiority of the NMCA algorithm over the other different optimisation algorithms.

Originality/value
The proposed approach is applied to solve the installation PVDG unit’s problem and reconfiguration problem in the radial distribution system, satisfying the operating and radial constraints. Also, it minimises active and reactive power losses and improves voltage profile.
The value-relevance of social media activity of Finnish listed companies
Authors: Rautiainen Antti , Jokinen Jonna 
Published: March 2022

Purpose
The use of social media tools by companies is common, but the links between the use of multiple social media tools by companies and stock price changes are largely unknown. Therefore, this study aims to analyze the value-relevance of social media activities on Facebook (FB), Instagram (IG), LinkedIn (LI), Twitter (TW) and YouTube (YT).

Design/methodology/approach
Stock market data and hand-picked social media data in this study were collected from Finland, a small language area with consistent International Financial Reporting Standards (IFRS) reporting practices, in the expectation of better comparability and lower noise in the data.This study uses correlation, regression and factor analyses for a sample of 105 Finnish public limited companies listed on the Nasdaq Helsinki stock exchange.

Findings
This paper finds evidence that social media activity is an important area of analysis and that the activity and popularity of a company in social media are value-relevant variables in forecasting stock prices.

Practical implications
Not all social media activities are necessarily equally important for managers and investors. Focus on visual messages in social media is recommended.

Originality/value
The findings of this study highlight the value-relevance of using multiple visual social media channels, particularly IG and YT. This paper suggests avenues for future research and for analyzing social media information.
Sustainable management of coastal critical infrastructure: case study of multi-purpose cyclone shelters in South Asia
Authors: Jaiswal Ankit , Kumar Anil , Pal Indrajit , Raisinghani Bhushan , Bhoraniya Tushar H. 
Published: March 2022

PurposeTo minimize risk of coastal communities arising from cyclones, several risk mitigation initiatives have been taken in countries. Cyclone shelters have proven to be an important critical infrastructure in saving lives from cyclones. A large number of coastal critical infrastructure in the form of multi-purpose cyclone shelters (MPCS) are built to provide safe shelter during disasters. Often observed, such critical infrastructures are non-operational during the normal period, which makes them difficult to use during any disaster. Efforts have been made to keep these infrastructures in working condition. This research paper aims to bring together various management practices adopted for the MPCS in the South-Asian region with a focus on Bangladesh, and India. It also suggests ways to improve these practices for sustainable management of the MPCS.Design/methodology/approachIndia and Bangladesh are the most vulnerable countries in the South Asian region. As per the Global Climate Index, India and Bangladesh come in the list of “in extreme risk” countries in the world and are vulnerable to several natural hazards, especially climate-induced hydrometeorological hazards. India has a vast coastline and out of 7,516 km of coastline, a large extent, i.e. 5,700 km is prone to cyclones and that keeps 40% of the population vulnerable living within 100 km of the coastline. On the other hand, Bangladesh has a coastline of 580 km, which is equally vulnerable to tropical cyclones. Safeguarding communities from impending coastal risk through coastal cyclone shelters are of prime concern. This paper uses a qualitative approach to analyze secondary data, and literature in the field of critical infrastructure, sustainability, cyclone shelter, and management practice for cyclone shelters.FindingsTo provide sustainability and community ownership of the MPCS, various service plans are adopted in different countries. This paper provides insights on service and sustainability efforts made for the proper functioning of the MPCS in India and Bangladesh. It also provides insight into the roles played by different institutions involved in maintaining the MPCSs.Originality/valueThe research reiterates understanding of the cyclone shelter management from different geographic locations in the South Asian region. Various gaps identified in shelter management practices are discussed in the paper and key recommendations are proposed for better management of cyclone shelters.
How scarcity influences home country consumers' attitudes toward the firm engaging in cross-border philanthropy? The role of perceived distributive justice
Authors: Tong Zelin , Ma Fang , Xiao Haowen , Haan Perry , Feng Wenting 
Published: March 2022

PurposeThe purpose of this research is to explore how experienced scarcity affects home country consumers' attitudes toward the firm engaging in cross-border philanthropy by analyzing perceived distributive justice as a mediating variable. This research also investigates the moderating factor of this effect to identify practical strategies for managers.Design/methodology/approachThis research conducted one survey (Study 1) and three experiments (Studies 2–4) by manipulating scarcity to provide robust evidence for the influence of experienced scarcity on consumer perception of the company conducting cross-border philanthropy.FindingsThis paper provides empirical insights about the significant negative effect of experienced scarcity on consumer attitudes toward the firm engaging in cross-border philanthropy. It proposes that home country consumers with high versus low experienced scarcity show lower perceived distributive justice for cross-border philanthropy, which generates less favorable attitudes toward the firm. To alleviate the negative impact of experienced scarcity on consumers' perceptions of corporate reputation, providing donation amount comparisons between home and foreign countries has a significant moderating effect.Practical implicationsThis paper provides several suggestions for marketers seeking cross-border philanthropy to improve consumers' attitudes toward the firm.Originality/valueThis paper enriches the literature on corporate social responsibility in the domain of cross-border philanthropy and explains contradictory findings on consumers' attitudes toward corporate cross-border philanthropy. Moreover, this study makes meaningful contributions to the scarcity and justice literature.
A consumer cultural paradox: exploring the tensions between traditional and international education
Authors: Beveridge Ivana , Furrer Olivier , Gelb Betsy D. 
Published: March 2022

PurposeIn a globalized world, consumers embrace mutually conflicting cultural values rather than making exclusive, either/or choices. As a result, they experience multiple tensions, a phenomenon that can be identified as the consumer cultural paradox. Despite clear interest in the influence of local/global culture on consumers, knowledge of how conflicting cultural elements shape consumer behavior remains limited. To address these issues, the current article seeks to identify higher- and lower-level tensions inherent in the consumer cultural paradox.Design/methodology/approachUsing in-depth interviews, the authors investigate tensions experienced by Chinese consumers of international private education services. This study applies a paradox lens, a tension-based conceptual approach that is well suited for studying consumer paradoxes.FindingsTen lower-level tensions of the consumer cultural paradox arise in the focal international service context; these tensions in turn form three higher-level tensions.Originality/valueThe study is among the first in marketing to use a paradox lens and empirical research to delineate multiple dimensions of the consumer cultural paradox, then categorize them into lower and higher-level tensions. The findings offer theoretical and managerial implications, in that recognizing the multiple tensions experienced by consumers allows scholars and marketers to gain a better understanding of how consumers perceive and evaluate services from different cultures.
Immigrant entrepreneurship with a focus on human and social capital as determinants of success: evidence from South Africa
Authors: Urban Boris , Murimbika McEdward , Mhangami Dennis 
Published: March 2022

Purpose
As a consequence of global changes, the landscape of immigration is changing. This brings opportunities for researching more nuanced aspects related to immigrant entrepreneurship in new contexts. The purpose of this paper is to establish the extent to which Africa-to-African immigrants leverage their social capital and human capital towards improving the success of their entrepreneurial ventures.

Design/methodology/approach
First-generation immigrant entrepreneurs within the Johannesburg area in South Africa were surveyed (n = 230). Instrument validity and reliability was first established, and then the hypotheses were tested using multiple regression analyses.

Findings
Hypotheses are supported insofar African immigrant entrepreneurs in South Africa rely on their structural and resource-related dimensions of social capital to achieve entrepreneurial success. Furthermore, human capital in terms of both work experience and entrepreneurial experience was found to be a significant predictor of entrepreneurial success.

Research limitations/implications
There is value in developing policies that promote African immigrant entrepreneurs with higher levels of human and social capital. These African immigrants have the potential to increase the national skills base and knowledge required for successful entrepreneurship development in South Africa.

Originality/value
While both human capital and social capital have been associated significantly with the generic entrepreneurship literature, this paper provides an empirical contribution by focusing on the relevance of these constructs in the context of immigrant entrepreneurship from an African emerging market perspective.