bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abnormal patellar loading may lead to femoral trochlear dysplasia: an experimental study of patellar hypermobility and patellar dislocation in growing rats
Authors: Tang Shiyu , Li Weifeng , Wang Shengjie , Wang Fei 
Published: December 2022

Abstract
Background: This animal study aimed to explore the effects of patellar hypermobility and patellar dislocation on the developing femoral trochlea.
Methods: Seventy-two 3-week-old Wistar rats were randomly divided into three groups. The sham group (SG) underwent simple incision and suture of the skin and subcutaneous tissue; the patellar hypermobility group (PHG) underwent medial and lateral retinacular release; the patellar dislocation group (PDG) underwent plication of the medial patellofemoral retinaculum. Twelve rats in each group were euthanized at 3 and 6 weeks postoperatively, respectively, and specimens were collected. The bony sulcus angle (BSA), cartilaginous sulcus angle (CSA), trochlear sulcus depth (TSD), and thickness of the cartilage on the lateral facet (CTL), medial facet (CTM), and center (CTC) of the trochlea were measured on hematoxylin and eosin-stained sections.
Results: In the PHG and PDG, the femoral condyles became blunt, the trochlear groove became shallower, and cartilage became thicker compared with the SG. Compared with the SG, the PHG and PDG had significantly larger BSA and CSA values at 3 (p < 0.05) and 6 weeks (p < 0.005), and a significantly shallower TSD (p < 0.05). At 3 weeks, all cartilage thicknesses in the PHG and the CTC and CTM in the PDG were significantly thinner than in the SG (PHG vs SG: p = 0.009 for CTL, p < 0.001 for CTM, p = 0.003 for CTC; PDG vs SG: p = 0.028 for CTC, p = 0.048 for CTM). At 6 weeks, the CTC was thicker in the PHG and PDG than the SG (PHG vs SG: p = 0.044; PDG vs SG: p = 0.027), and the CTL was thinner in the PDG than the SG (p = 0.044).
Conclusion: Patellar hypermobility and patellar dislocation may result in trochlear dysplasia that worsens with age. Excessive or insufficient loading leads to trochlear dysplasia.
Isotretinoin treatment upregulates the expression of p53 in the skin and sebaceous glands of patients with acne vulgaris
Authors: Agamia Naglaa Fathi , ElMulla Khalid Fawzi , Ghazala Rasha Ahmed , Alsayed Naglaa Mohamed , Abdelmaksoud Rania Elsayed , Elmonem Eman Abdel , Talat Eman Mahmoud , Zaki Inas Naser , Sabah Rana Mohamed , Melnik Bodo Clemens 
Published: December 2022

Abstract
The transcriptomic regulation induced by isotretinoin (13-cis retinoic acid) is still a matter of debate as short-term exposures of immortalized sebocytes with isotretinoin produced conflicting results. Based on translational evidence, it has been hypothesized that oral isotretinoin treatment upregulates the expression of the transcription factor p53. Twenty-five patients suffering from acne vulgaris were treated with isotretinoin (0.6 mg/kg body weight) for 6 weeks. Biopsies from back skin were taken before and after isotretinoin treatment for the determination of p53 expression by immunohistochemical staining, quantification of p53 protein concentration by enzyme-linked immunosorbent assay and TP53 gene expression by quantitative reverse transcription real time PCR. Fifteen socio-demographically cross-matched healthy volunteers served as controls. Isotretinoin treatment significantly increased the nuclear expression of p53 in sebaceous glands of treated patients compared to pre-treatment levels and p53 levels of untreated controls. Furthermore, the p53 protein and gene expression significantly increased in the skin after treatment. The magnitude of p53 expression showed an inverse correlation to acne severity score and body mass index. Under clinical conditions, isotretinoin induced the expression of p53, which controls multiple transcription factors involved in the pathogenesis of acne vulgaris including FoxO1, androgen receptor and critical genes involved in the induction of autophagy and apoptosis. Increased p53-FoxO1 signalling enhanced by systemic isotretinoin treatment explains the underlying transcriptomic changes causing sebum suppression but also the adverse effects associated with systemic isotretinoin therapy.
Advances in mechanistic study of control of oxidative stress injury through modulating HDAC6 activity
Authors: Xue Yuanye , Gan Bing , Zhou Yanxing , Wang Tingyu , Zhu Tong , Peng Xinsheng , Zhang Xiangning , Zhou Yanfang 
Published: December 2022

Abstract
Oxidative stress is defined as injury resulting from the disturbance on dynamic equilibrium of redox environment due to over-production of active/ radical oxygen exceeding the anti-oxidative ability in the body. It is a key step of genesis and development of various diseases. Oxidative stress is modulated by different factors and events, including modification of histone, the core of nucleosome. Modification of histone includes acetylation and deacetylation on certain amino acid residues; the process is catalyzed by different enzymes. Histone deacetylase 6 (HDAC6) is a unique deaetylating protease; it also catalyzes deacetylation of different non-histone substrates so as to regulate various physiologic processes. The intimate relationship between HDAC6 and oxidative stress has been demonstrated by different lines of study. The present paper aims at summarizing the data obtained from mechanistic study between HDAC6 and oxidative stress, in order to provide guidance for further investigations in term of mechanistic characterization and drug development.
Non-conventional colloidal aggregation in bacterial baths
Authors: Palacci Jérémie , Grober Daniel , Palaia Ivan , Ucar Mehmet Can , Hannezo Edouard , Šarić Anđela 
Published: December 2022

Abstract
In equilibrium, thermal forces agitate molecules that diffuse, collide, and bind to form materials. They are, however, insufficient to efficiently organize particles at the micron-scale due to slow dynamics and metastable states [1, 2]. Active agents present in a passive fluid generate forces and flows: a bath with active fluctuations[3, 4, 5, 6], whose use for synthetic assembly is primarily untapped. We investigate the aggregation of sticky colloids in an active bath of swimming E. Coli bacteria. We observe a slow but persistent rotation of the aggregates that originates in the chirality of the E.Coli flagella and directs aggregation into structures that are not accessible thermally. We elucidate the aggregation mechanism with a numerical model of spinning, sticky beads and reproduce quantitatively the experimental results. We show that internal activity controls the phase diagram and the structure of the aggregates. Taken together, our results highlight the promise of active baths for designing a new class of materials whose structural and mechanical properties are not accessible to thermal assembly.
Numerical Investigation of Water Inflow and Seepage Characteristics in a Tunnel crossing Two Overlapped Parallel Faults
Authors: Wu Jing , Wu Li , Han Yan-hua , Sun Miao , Lu Ya-ni 
Published: December 2022

Abstract
Water inflow is one of the main geohazards that threaten the safety of tunnels and other underground projects. Faulted zone is one of the important geological triggers for such events. Numerical investigations on the evolution of flow behavior in tunnels across fault zones are of significance to the predication and prevention of this type of geohazards. In this work, a numerical investigation model with two overlapped parallel faults is established according to the "Three Zones" fault structure theory. The rapid turbulent flow in the fault fractured zone and fault center core is simulated by using the improved Darcy-Brinkman (IDB) seepage model, while the slow laminar flow in ordinary rock zone is described by Darcy equation. The effect of relative position and distance between the tunnel surface and two overlapped parallel faults to the evolutions of pore pressure(P), flow velocity(V), and water inflow rate(Q) around the tunnel excavation surface is studied through several scenarios. Our Numerical investigation results reveals that while the tunnel face is excavated into the fault center core, the fractured zone, the ordinary rock zone, and the center of the overlapped faults, the pore pressure value ahead of the excavation face increases while the flow velocity decreases sequentially. The inflow rate is the largest while the tunnel face is excavated to center of the fault center core and is the lowest in the ordinary rock zone. Moreover, the inflow rate is closely related to the range of the overlapped area between the two parallel faults. The investigation results offer a helpful reference to predicting early warning of water inflow geohazard when a tunnel cross two overlapped parallel faults.
Approach to the prevalence and comorbidity of neurodevelopmental disorders in primary care consultations on the island of Menorca in 6-year-old children
Authors: Francés Lorena , Caules Jessica , Ruiz Antoni , Soler C. Virgínia , Hervás Amaia , Fernández Alberto , Rodríguez-Quiroga Alberto , Quintero Javier 
Published: December 2022

Abstract
Background
Few studies estimate the real prevalence of neurodevelopmental disorders according to DSM-5 in Spain and worldwide. However, there are disparate prevalence figures. We consider research in this field essential to improve early detection, secondary prevention, and health planning.
Methods
The Minikid ADHD and TICS- Mini International Neuropsychiatric Interview for Children and Adolescents, The Autism Spectrum Quotient (Children’s version, AQ- Child) and a protocol of general medical questions were administered for screening purposes. The PROLEXIA battery in ages between 4–6 years was used to directly assess children. Parents provided information on emotional, medical, and school aspects. The final population evaluated using these tools was made up of 291 6-year-old subjects.
Results
The overall risk of presenting a neurodevelopmental disorder was 55.4% of the sample. A 23.4% risk of presenting attention deficit hyperactivity disorder (ADHD) in any modality (inattentive, hyperactive-impulsive and combined), a 2.8% risk of developing ASD, a 30.6% risk of presenting a learning disorder with reading difficulties, a 5.5% risk of tics and a 22.5% of language problems (incomprehensible language or minor language problems) were detected. The most common combinations were learning and language difficulties in 6.9% of the sample. The second most frequent combination was the presence of learning and language difficulties and the risk of ADHD in 4.5%.
Conclusions
The prevalence of risks detected in our sample seems to be consistent with national and international studies. A significant proportion had never been previously diagnosed, so it would be convenient to insist on early detection programs.
Should ICSI be implemented on patients with poor-quality embryos in the previous IVF cycle?
Authors: Wang Jiang , Xiong Shun , Xia Fei , Liao Haiyuan , Wei Biao , Zou Jiayi , Han Wei 
Published: December 2022

Abstract
Purpose
This study was to evaluate whether ICSI can improve the quality of embryo in patients with poor-quality embryos in the previous IVF cycle.
Methods
This was a retrospective cohort study of 178 IVF and 158 ICSI cycles for patients with poor-quality embryos in the previous IVF cycle in the Center for Reproductive Medicine, Women and Children’s Hospital of Chongqing Medical University from March 2016 to June 2022. The 2PN rate, oocyte utilization rate and high-quality embryo rate and clinical pregnancy rate were compared between the two groups. Other outcome measures included the implantation rate, miscarriage rate and cycle cancelation rate.
Results
Compared with IVF, ICSI resulted in a similar 2PN rate, oocyte utilization rate and cycle cancelation rate. The high-quality embryo rate of ICSI group was significantly higher than that of IVF group (5.56% vs. 2.60%, P < 0.05). Among of them, a total of 239 patients performed embryo transfer, ICSI resulted in a significantly higher clinical pregnancy rate (55.56% vs. 40.98%, P < 0.05) compared with IVF, but there were no notable differences in miscarriage rate and implantation rate.
Conclusion
The present study suggested that ICSI can significantly improve the quality of embryo and clinical pregnancy of the patients with poor-quality embryos in the previous IVF cycle.
A geochemical approach in assessing seawater intrusion by integrating geospatial techniques: a case study of Ghiss-Nekor coastal aquifer, Central Rif of Morocco
Authors: BOURJILA Abdelhak , DIMANE Fouad , GHALIT Mohammad , TAHER Morad , KAMARI Salim , ACHOUKHI Iliass , HAMMOUDANI Yahya EL , SAADI Omar , HABOUBI Khadija 
Published: December 2022

Abstract
For effective coastal aquifers management, it is strongly required to effectively analyze seawater intrusion (SWI). This study used an integrated approach of hydrogeochemical, statistical, geological, and geospatial techniques to assess the extent of SWI in the Ghiss-Nekor aquifer by evaluating the physicochemical parameters of 52 groundwater samples. Two main groundwater facies were identified, Na-Cl (38%) and Ca-Mg-Cl-SO4 (62%). The correlation matrix and a principal component analysis (PCA) depicted that the high salinization in the study area is influenced by both geogenic and anthropogenic factors, including a potential mixing with seawater. A single indicator or a small number of techniques were insufficient to evaluate SWI owing to the multiple causes of salinization in the study area. As a result, we coupled various geochemical indicators with geospatial methods to assess this complicated phenomenon. Accordingly, several ionic ratios (Cl/HCO3, SO4/Cl, Na/Cl, and Mg/Mg+Ca) and SWI indices (GQIswi and SMI) were overlaid to generate the final map that highlights the regions prone to SWI. Most of the SWI spots were discovered within two kilometers or less from the coast. The saline water detected far from the coast was interpreted as the encroachment of seawater from the Souani area being trapped where the clay-marly substratum is deep. These results support the application of geospatial tools to manage groundwater resources in water-stressed areas with complex aquifer systems, by combining various ionic ratios and indices. These findings will assist decision-makers in the Al Hoceima region in developing suitable groundwater management plans and strategies.
IDH mutations in G2-3 conventional central bone chondrosarcoma: a mono institutional experience
Authors: Setola Elisabetta , Benini Stefania , Righi Alberto , Gamberi Gabriella , Carretta Elisa , Ferrari Cristina , Avnet Sofia , Palmerini Emanuela , Magagnoli Giovanna , Gambarotti Marco , Lollini Pier Luigi , Cesari Marilena , Cocchi Stefania , Paioli Anna , Longhi Alessandra , Scotlandi Katia , Laginestra Maria Antonella , Donati Davide Maria , Baldini Nicola , Ibrahim Toni 
Published: December 2022

Abstract
Background: Heterozygous isocitrate dehydrogenase (IDH) mutations occur in about half of conventional central bone chondrosarcomas (CCBC). Aim of this study was to assess the frequency and prognostic impact of IDH mutations in high grade CCBC patients.
Methods: 64 patients with G2 and G3 CCBC were included. DNA extraction, PCR amplification of IDH1/2 exon 4s, and sequencing analysis with Sanger were performed.
Results: IDH mutations were detected in 24/54 patients (44%): IDH1 in 18, IDH2 in 4, and both IDH1/2 in 2 patients. The frequency of mutations was 37% in G2 vs. 69% in G3 (p = 0.039), and 100% in three Ollier disease associated chondrosarcoma.
5-year overall survival (OS) at 124 months (range 1-166) was 51%, with no significant difference based on the IDHmutational status: 61% in IDHmut vs 44% in IDH wild type (IDHwt). The 5-year relapse free survival (RFS) was 33% (95% CI:10-57) for IDHmut vs 57% (95%CI: 30-77) for IDHwt. Progression free survival (PFS) was 25% (95%CI:1-65) IDHmut vs. 16% (95%CI: 0.7-52) IDHwt. 55% (5/9) of IDHmut G2 became higher grade at the recurrence, as compared with 25% (3/12) of G2 IDHwt.
Conclusions: This study shows a higher frequency of IDH mutations in G3 CCBC as compared with G2. No significant differences in OS, RFS, and PFS by mutational status were detected. After relapse, a higher rate of G3 for IDH mutated CCBC was observed.
Physical activity and eating behaviors patterns associated with high blood pressure among Chinese children and adolescents
Authors: CHEN Jiaoyan , WANG Yuanyuan , LI Wenxiu , ZHANG Ya , CAO Ruiyao , PENG Xingwang , ZHANG Juan , LIU Keke , HAN Mei , FU Lianguo 
Published: December 2022

Abstract
Background
Physical activity and eating behavior are associated with hypertension in children and adolescents. Revealing the associations between physical activity patterns, eating behavior patterns and high blood pressure (HBP) could help improve the problem of hypertension from the actual children’s physical activities and eating behaviors.
Methods
The students aged 8–15 years was selected from two nine-year primary and secondary schools using stratified cluster random sampling method. The students’ body height, weight, systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured, and their physical activity time and eating behaviors were surveyed by using CLASS questionnaire and self-made eating behavior questionnaire. Exploratory factor analysis (EFA) was used to extract moderate-high intensity physical activity factor (MHPAF), sedentary physical activity factor (SPAF), healthy eating behavior factor (HEBF), unhealthy eating behavior factor (UHEBF). MHPAF ≥ SPAF was defined as moderate-high intensity physical activity pattern (MHPAP), MHPAF < SPAF was defined as sedentary physical activity pattern (SPAP). HEBF ≥ UHEBF was defined as healthy eating behavior pattern (HEBP), while the opposite was defined as unhealthy eating behavior pattern (UHEBP).
Results
The MHPAF and UHEBF in boys were significantly higher than those in girls (P < 0.01), while the SPAF in girls was significantly higher than that in boys (P < 0.05). The SPAF was positively correlated with SBP in girls (β (SE) = 1.36 (0.50), P = 0.07), and was positively correlated with SBP (β (SE) = 4.81 (1.22), P < 0.001) and DBP (β (SE) = 1.36 (0.49), P = 0.006) in boys. The MHPAF was negatively correlated with DBP (β(SE)=-0.94 (0.41), P = 0.022) in boys. In boys, the SPAP increased the risks of HBP (OR (95% CI):3.34 (1.30–8.63)) and high DBP (OR (95% CI):3.08 (1.02–9.34)) compared with MHPAP.
Conclusion
Compared with the boys with MHPAP, boys with SPAP may increase the risks of HBP and high DBP. The SPAF may be positively associated with SBP in boys and girls, while the MHPAF may be negatively associated with DBP in boys.
Changes of hyperopia reserve and other eye parameters among Chinese children aged 3-6 years old
Authors: Yu Wang , Zhu Hong , Xu Yong , Gao Hongqin , Zhu Guangwei , Qian Dengjuan , Pan Hong , Li Juan 
Published: December 2022

AbstractPurpose Track refractive changes in preschool children to explore the characteristics of refractive changes, further delineate refractive status and provide data for the prevention of refractive errors in preschool children. Methods The refractive data of 26,528 preschoolers in 2021 and 16383 ones lasting for 2 years(2020 and 2021) aged 3–6 years were collected from different kindergartens in Suzhou High-tech Zone for the study(Until 23 July 2021). The refractive data of every participant was measured with the Welch Allyn Spot on non-cycloplegic refraction. A follow-up for all the included participants was performed. The spherical equivalent change was calculated, subsequently, the characteristics related to the change were performed. Results The chi-square test of 26528 preschoolers in 2021 showed a higher prevalence of hyperopia in boys (χ2 = 4.299,P = 0.038), and a higher prevalence of hyperopia reserve deficiency in boys (χ2 = 11.987,P = 0.001). And there are the trends for myopia (χ2 = 26.398,P < 0.001), astigmatism (χ2 = 58.838,P < 0.001), hyperopia reserve deficiency (χ2 = 24.290,P < 0.001) to increase with age. Trackable data shows the incidence of refractive errors (= Number of new patients in 2021/number of non-patients in 2020). After Chi-square that the incidence of hyperopia, astigmatism and myopia was statistically different at different ages and the incidence of the three at 5 years-old are at the high level. The further analysis of the newly developed people with hyperopia reserve deficiency shows the decline rate of hyperopia reserve has slowed down with age, the rate of decline of 2–3 and 3–4 are faster than 4-5and 5–6 significantly. Conclusion In preschool, boys possess higher hyperopia than girls; and, as age increases, but the increase rate of hyperopia reserve deficiency for girls is faster with the growth of age. There were the special incidence of hyperopia and astigmatism at 5 years old. And we should watch out for sharp changes in early hyperopia reserve. At the same time, the serious lack of hyperopia reserve(19%) of children in Suzhou also sounded an alarm for myopia prevention.
Early efficacy of rehabilitation training after surgery for lumbar disc herniation
Authors: Zhu Yuwei , Xia Shuang , Yang Weihang , Shi Fengchao 
Published: December 2022

Abstract
Background
To study the early clinical efficacy of Unilateral Biportal Endoscopy (UBE) in the treatment of lumbar disc herniation after surgery.
Methods
A total of 100 patients with lumbar disc herniation who underwent UBE technology in Yancheng Third People's Hospital from January 2019 to January 2021 were retrospectively analyzed and randomly divided into the intervention group (N = 50) and the control group (N = 50). The control group was given routine functional exercise, while the intervention group was given rehabilitation training on the basis of routine functional exercise. The pain degree and quality of life in the early postoperative period were compared between the two groups, and the risk factors affecting the prognosis of patients were analyzed.
Results
The postoperative VAS, ODI scores and quality of life in the two groups were significantly decreased (P < 0.05). In postoperative follow-up, the VAS and ODI scores of the intervention group were lower than those of the control group at 3 and 12 months after operation, and the differences were statistically significant (P < 0.05). The quality of life score of the intervention group was lower than that of the control group 12 months after operation, and the difference was statistically significant (P < 0.05). At 12 months after operation, the excellent and good rate of the modified Macnab standard intervention group was 88.00%, and that of the control group was 62.00%. The difference of the excellent and good rate of the modified Macnab standard after operation between the two groups was statistically significant (P < 0.05). The results of Logistic multivariate regression model analysis showed that the postoperative rehabilitation (95%CI: 1.360-12.122, P = 0.012), the degree of intervertebral disc (95%CI: 0.010–0.676, P = 0.020), and the age (95%CI: 1.360-12.122, P = 0.012). 1.056–8.244, P = 0.039) was an independent risk factor affecting the prognosis of patients.
Conclusion
Routine functional exercise is recommended for patients with lumbar disc herniation surgery, which can effectively reduce pain and improve quality of life.