bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrin β1, PDGFRβ, and type II collagen are essential for meniscus regeneration by synovial mesenchymal stem cells in rats
Authors: Kitahashi Tsukasa , Kogawa Ryo , Nakamura Kentaro , Sekiya Ichiro 
Published: August 2022

AbstractSynovial mesenchymal stem cells (MSCs) injected into the knee promote meniscus regeneration in several animal models; however, the mode of action is unknown. Our purpose was to identify the molecules responsible for this meniscus regeneration. Rat synovial MSCs were treated with neutralizing antibodies for integrin β1, PDGFRβ, or CD44 or with the CRISPR/Cas9 system to delete Vcam1, Tnfr1, or Col2a1 genes. After partial meniscectomy, rat knees were injected with MSCs, and the regenerated meniscus area was quantified three weeks later. The in vivo and in vitro functions were compared between the treated and control MSCs. Anti-integrin β1 neutralizing antibody inhibited in vitro MSC adhesion to collagen-coated chambers, anti-PDGFRβ neutralizing antibody inhibited proliferation in culture dishes, and Col2a1 deletion inhibited in vitro chondrogenesis. In vivo, the regenerated meniscus area was significantly smaller after injection of MSCs treated with integrin β1 and PDGFRβ neutralizing antibodies or lacking type II collagen gene than after control MSC injection. By contrast, the regenerated areas were similar after injection of control, CD44-, Vcam1-, or Tnfr1 treated MSCs (n = 12–16) MSCs. Synovial MSCs injected into the knee joint promoted meniscus regeneration by adhesion to integrin β1 in the meniscectomized region, proliferation by PDGFRβ, and cartilage matrix production from type II collagen.
Initial experience of a deep learning application for the differentiation of Kikuchi-Fujimoto’s disease from tuberculous lymphadenitis on neck CECT
Authors: Kim Byung Hun , Lee Changhwan , Lee Ji Young , Tae Kyung 
Published: August 2022

AbstractNeck contrast-enhanced CT (CECT) is a routine tool used to evaluate patients with cervical lymphadenopathy. This study aimed to evaluate the ability of convolutional neural networks (CNNs) to classify Kikuchi-Fujimoto’s disease (KD) and cervical tuberculous lymphadenitis (CTL) on neck CECT in patients with benign cervical lymphadenopathy. A retrospective analysis of consecutive patients with biopsy-confirmed KD and CTL in a single center, from January 2012 to June 2020 was performed. This study included 198 patients of whom 125 patients (mean age, 25.1 years ± 8.7, 31 men) had KD and 73 patients (mean age, 41.0 years ± 16.8, 34 men) had CTL. A neuroradiologist manually labelled the enlarged lymph nodes on the CECT images. Using these labels as the reference standard, a CNNs was developed to classify the findings as KD or CTL. The CT images were divided into training (70%), validation (10%), and test (20%) subsets. As a supervised augmentation method, the Cut&Remain method was applied to improve performance. The best area under the receiver operating characteristic curve for classifying KD from CTL for the test set was 0.91. This study shows that the differentiation of KD from CTL on neck CECT using a CNNs is feasible with high diagnostic performance.
Adopting Change and Incorporating Technological Advancements in Audiology Education, Research, and Clinical Practice
Authors: Morgan Shae D. , Zeng Fan-Gang , Clark Jackie 
Published: August 2022

Purpose:
With the rapid development of new technologies and resources, many avenues exist to adapt and grow as a profession. Embracing change can lead to growth, evolution, and new opportunities. Audiologists have the potential to harness many of these technological advancements to improve patient health care. Adoption and incorporation of these new technologies will likely benefit educational experiences, research methods, clinical practice, and clinical outcomes.

Method:
This commentary highlights some historical perspectives and accepted practices while illustrating opportunities to embrace new ideas and technologies. We also provide examples of how such adoption may yield positive outcomes. Specifically, we address embracing technology in audiology education, how artificial intelligence may influence patient performance in realistic listening scenarios, the convergence between hearing aids and consumer electronics, and the emergence of audiology telehealth services and their inclusion in clinical practice. Models of change are also discussed and related to audiology.

Conclusion:
This commentary aims to be a call to action for the entire profession of audiology to consider conscientiously the adoption of useful, evidence-based technological advancements in education, research, and clinical practice.
A Study on Reliability and Validity of the Simplified Chinese Version of the Trans Woman Voice Questionnaire
Authors: Wang Lu , Zhang Jiahe , Zhang Qingfeng , Wang Chenxi , Zhang Xiang , Hu Rongqing , Jin Yukun , Ding Huijun , Chen Zhen 
Published: August 2022

Purpose:This study aims to translate the English version of the Trans Woman Voice Questionnaire (TWVQ) to simplified Chinese (TWVQ-SC) and to examine its reliability and validity.Method:Standardized translation procedures were strictly followed for the translation of the TWVQ. Two hundred sixty trans woman and 128 cis woman subjects completed sociodemographic investigation, the TWVQ-SC, and the Voice Handicap Index–10 (VHI-10) online. Internal consistency was examined by Cronbach reliability coefficient (Cronbach α). Test–retest reliability was quantified by intraclass correlation coefficient (ICC). Content validity, structural validity, and discriminant validity were examined by expert panel's judgment, factor analysis, Spearman's rank correlation coefficient, and the Mann–WhitneyUtest.Results:The Cronbach α of the TWVQ-SC was .969 and the ICC was .841, indicating excellent internal consistency and good test–retest reliability. The four principal factors explained 21.345%, 18.592%, 13.551%, and 12.027% of the variance respectively with the cumulative contribution rate 65.514%. There was a strong correlation between the total score of the TWVQ-SC and that of the VHI-10 (r= .858,p< .001), indicating good structural validity. The total score of the TWVQ-SC of the trans woman subjects was significantly higher than that of the cis woman subjects (z= 14.590,p< .05), indicating good discriminant validity.Conclusion:The TWVQ-SC exhibits overall high reliability and validity, qualified to be applied as a reliable clinical tool to evaluate trans women's voice in mainland China.
Behavioral Parent Training for Families With Young Deaf or Hard of Hearing Children Followed in Hearing Health Care
Authors: Studts Christina R. , Jacobs Julie A. , Bush Matthew L. , Lowman Joneen , Westgate Philip M. , Creel Liza M. 
Published: August 2022

Purpose:
It is well established that individuals with a communication disability, including being deaf or hard of hearing (DHH), experience inequities in health services and outcomes. These inequities extend to DHH children's access to psychosocial evidence-based interventions (EBIs). Behavioral parent training is an EBI that can be used to improve caregiver and child outcomes. Despite being supported by decades of effectiveness research, this EBI is rarely accessed by, or studied with, caregivers of DHH children. The purpose of this article is to describe a program of stakeholder-engaged research adapting and assessing behavioral parent training with caregivers of young DHH children followed in hearing health care, aimed at reducing inequities in access to this EBI.

Method:
The first section briefly summarizes the literature on disruptive behavior problems in young children, with a focus on preschool-age DHH children. The evidence base for behavioral parent training is described. Next, the gaps in knowledge and practice regarding disruptive behaviors among DHH children are highlighted, and the potential integration of behavioral parent training into the standard of care for this population is proposed.

Conclusions:
Young DHH children who use hearing aids and/or cochlear implants experience disruptive behavior problems at rates at least as high as typically hearing children, but their access to EBIs is limited, and behavioral parent training programs tailored to this population have not been rigorously tested. Caregivers and hearing health care service providers affirm the potential benefits of behavioral parent training and were partners in adapting this EBI. This research highlights several principles and approaches essential for reducing inequities and improving the quality of life not only for DHH children and their families but also for individuals with communication disabilities more broadly: engagement of key stakeholders in research, collaboration across disciplines, and using implementation science methods and models to design for implementation, dissemination, and sustainment.

Presentation Video:

https://doi.org/10.23641/asha.21215900
A curcumin direct protein biosensor for cell‐free prototyping
Authors: Kennedy Agata , Griffin Guy , Freemont Paul S. , Polizzi Karen M. , Moore Simon J. 
Published: August 2022

AbstractIn synthetic biology, biosensors are routinely coupled with a gene expression system for detecting small molecules and physical signals. We reveal a fluorescent complex, based on the interaction of an Escherichia coli double bond reductase (EcCurA), as a detection unit with its substrate curcumin—we call this a direct protein (DiPro) biosensor. Using a cell‐free synthetic biology approach, we use the EcCurA DiPro biosensor to fine tune 10 reaction parameters (cofactor, substrate, and enzyme levels) for cell‐free curcumin biosynthesis, assisted through acoustic liquid handling robotics. Overall, we increase EcCurA‐curcumin DiPro fluorescence within cell‐free reactions by 78‐fold. This finding adds to the growing family of protein–ligand complexes that are naturally fluorescent and potentially exploitable for a range of applications, including medical imaging to engineering high‐value chemicals.
Stacking ensemble learning with heterogeneous models and selected feature subset for prediction of service trust in internet of medical things
Authors: Ren Junyu , Wan Haibin , Zhu Chaoyang , Qin Tuanfa 
Published: August 2022

AbstractRecently, with the fast development of IoT, Internet of medical things (IoMT) has drawn wide attention from both industry and academia. However, pressing challenges exist in practical implementation of IoMT, such as service provision with stringent latency. To address the challenges, fog computing is generally employed in IoMT systems. However, it raises additional concerns of trust and security. To tackle the issue, the authors introduce the security measure of trust into this work, and a superior heterogeneous stacking ensemble learning measure for trustworthiness prediction (SEM‐TP) of fog services is proposed. Besides, to reduce unnecessary time cost incurred by unimportant features, an efficient voting‐based feature selection (FS) strategy called voting‐based feature selection method is proposed to select significant features, which is based on diverse FS measures. Extensive experiments are conducted and the results show that the proposed framework outperforms commonly used single classifiers and competing stacking models in terms of Accuracy, Precision, Recall, F1‐score, Kappa coefficient, and Hamming distance under different conditions, validating the effectiveness, robustness, and superiority of the proposed trustworthiness prediction and FS methods.
London Dispersion Stabilizes Chloro-Substituted cis-Double Bonds
Authors: Schreiner Peter R. , Rummel Lars , Hanke Kai , Becker Jonathan 
Published: August 2022

AbstractWe present a combined experimental and computational study on the thermodynamic stability of cis- and trans-alkenes substituted with dispersion energy donor (DED) groups. To investigate the role of noncovalent interactions on equilibrium of cis- and trans-alkenes we utilized hydrochlorination reactions. While the general assumption is that increasing steric bulk favors the trans-alkene, we observe an equilibrium shift towards the more crowded cis-alkene with increasing substituent size. With the aim to quantify noncovalent interactions, we performed a double mutant cycle to experimentally gauge the attractive potential of bulky substituents. Additionally, we utilized local energy decomposition analysis at the DLPNO-CCSD(T)/def2-TZVP level of theory. We found LD interactions and Pauli exchange repulsion to be the most dominant components to influence cis- and trans-alkene equilibria.
Agile offset frequency locking for single-frequency fiber lasers
Authors: Wang Enlong , Wang Guochao , Yu Xiao , Ying Kang , Yang Mingyue , Zhang Xu , Li Xuan , Yan Shuhua , Yang Jun , Zhu Lingxiao 
Published: August 2022

Single frequency fiber lasers (SFFLs) have seen increasing applications in state-of-the-art quantum technologies, which usually require precise and stable offset frequency locking (OFL). However, limited by the piezoelectric transducer bandwidth in SFFLs and the loop bandwidth of the OFL, the large-gap jumping between two locked offset frequencies will take an undesirable amount of time. In order to diminish that consuming time, we developed an agile offset frequency locking system based on a hybrid loop of a feed-forward path and a feedback path. In accordance with the experimental demonstration, we characterized the performances of the offset frequency locking system, as frequency-locking stability with an Allan deviation of 3.2 × 10−14 at 1 s averaging time and jumping agility with a duration of 0.6 ms at 1.3 GHz frequency gap, which is a factor of 60 faster than that without the feed-forward path. This mechanism can find direct applications in existing quantum metrology experiments with SFFLs where high-speed frequency jumping or sweeping is needed.
Thomson scattering on the large plasma device
Authors: Ghazaryan S. , Kaloyan M. , Gekelman W. , Lucky Z. , Vincena S. , Tripathi S. K. P. , Pribyl P. , Niemann C. 
Published: August 2022

We have developed a non-collective Thomson scattering diagnostic for measurements of electron density and temperature on the Large Plasma Device. A triple grating spectrometer with a tunable notch filter is used to discriminate the faint scattering signal from the stray light. In this paper, we describe the diagnostic and its calibration via Raman scattering and present the first measurements performed with the fully commissioned system. Depending on the discharge conditions, the measured densities and temperatures range from 4.0 × 1012 to 2.8 × 1013 cm−3 and from 1.2 to 6.8 eV, respectively. The variation of the measurement error with plasma parameters and discharges averaged is also discussed.
A photonic crystal fiber broadband mode converter with highly fitting propagation constant
Authors: Ding Yuxin , Li Jianshe , Li Shuguang , Wang Xiaokai , Guo Ying , Meng Xiaojian , Zhao Yuanyuan , Du Huijing 
Published: August 2022

Abstract
In this paper, we propose a broadband mode converter based on photonic crystal fiber (PCF) starting from the method of propagation constant height fitting. Using the finite element method and the beam propagation method, three typical mode converters in the S + C + L range, LP01 to LP11, LP01 to LP21, and LP01 to LP31, are calculated. Numerical results show that the mode coupling efficiency of this mode converter is over 90% and the mode extinction ratio is over 10 dB. This converter can realize pattern multiplexing in a cascade manner. On this basis, we propose a multi-core mode multiplexer structure. On the premise of sacrificing some performance, three modes can be reused at the same time. Mode converters of PCF structure can be fabricated by PCF post-processing technology. It is expected to become an important part of the future mode division multiplexing (MDM) optical communication system.
Screening and collective effects in randomly pinned fluids: a new theoretical framework
Authors: Phan Anh D 
Published: August 2022

Abstract
We propose a theoretical framework for the dynamics of bulk isotropic hard-sphere systems in the presence of randomly pinned particles and apply this theory to supercooled water to validate it. Structural relaxation is mainly governed by local and non-local activated process. As the pinned fraction grows, a local caging constraint becomes stronger and the long range collective aspect of relaxation is screened by immobile obstacles. Different responses of the local and cooperative motions results in subtle predictions for how the alpha relaxation time varies with pinning and density. Our theoretical analysis for the relaxation time of water with pinned molecules quantitatively well describe previous simulations. In addition, the thermal dependence of relaxation for unpinned bulk water is also consistent with prior computational and experimental data.
Stark-localization as a probe of nanostructure geometry
Authors: Pedersen T G , Cornean H D , Krejčiřik D , Raymond N , Stockmeyer E 
Published: August 2022

Abstract
It is a central tenet of quantum mechanics that spatial resolution is limited by the wave nature of particles. Energies of stationary states reflect delocalized wave functions and cannot be ascribed to any single point. Yet, electrons confined in nanostructures become localized against the boundary by strong electric fields. Energies then reflect the local curvature of the nanostructure surface rather than entire volume. We propose using spectroscopy of Stark-localized states to map nanostructure surface curvature. By varying field direction, local curvatures are extracted from absorption spectra. Moreover, the required field strength is shown to be feasible experimentally. We use nanowires with elliptic cross section as a detailed benchmark providing quantitative error estimates and practical guide lines.
Effect of laser irradiation protocols on temperature, roughness, and morphology of the root surface after root-end resection
Authors: Fiscarelli Victória Maria , Silva Ana Lídia Pinheiro , de Oliveira Eliane Cristina Gulin , Zaniboni Joissi Ferrari , Besegato João Felipe , Faraoni Juliana Jendiroba , Kuga Milton Carlos , Berbert Fábio Luiz Camargo Vilella 
Published: August 2022

Abstract
This study evaluated the effect of laser irradiation protocols using Thera Lase Surgery (TL), at 2.5 W (2.5) or 2.7 W (2.7), and Gemini (GE), at 1.2 W (1.2) or 1.5 W (1.5) of potency, on the root-end resection surface, in relation to external root temperature, roughness, and surface morphologic changes after the treatments, in the areas near to the root canal (RC) or external root surface (ER). Fifty teeth were endodontically treated and the root-end were resected 3 mm away from the root apex. The specimens were randomized into 5 groups (n = 10), according to the laser irradiation protocol: Control (CO-DW) without irradiation; (TL-2.5) Thera Lase Surgery, 2.5 W; (TL-2.7) Thera Lase Surgery, 2.7 W; (GE-1.2) Gemini, 1.2 W; and (GE-1.5) Gemini, 1.5 W. The external root temperature change was obtained through the difference between the highest value observed during the irradiation and the value at the beginning of the treatment. The surface roughness (µm) was analyzed by confocal laser microscopy and the apical surface morphology was evaluated by scanning electron microscopy, in RC or ER. The temperature change was similar between the irradiation protocols (p > 0.05). CO-DW and GE-1.5 provided the lowest surface roughness (p < 0.05), regardless of the analyzed area. TL-2.7 and GE-1.7 showed more uniform on apical resected morphology and a higher incidence of dentinal tubules occlusion, but only near the root canal (p < 0.05). Root-end resected surface submitted to TL-2.7 and GE1.7 irradiation protocols are more homogeneous and shown higher incidence of closed dentinal tubules near to root canal, despite providing a surface roughness.
Effect of 3 T magnetic field on RF plasma sputtering in an ITER-relevant first mirror unit
Authors: Soni Kunal , Iyyakkunnel Santhosh , Steiner Roland , Antunes Rodrigo , Moser Lucas , Bieri Oliver , Marot Laurent , Meyer Ernst 
Published: August 2022

Abstract
The first mirror (FM) cleaning operations in ITER are expected to be executed in the presence of a

∼
3

T magnetic field. In the RF plasma cleaning configuration, this would have a significant influence on the plasma properties, ion energy, angle of incidence as well as flux spatial distribution. To this end, RF discharges were excited in an ITER-sized mock-up of a first mirror unit (FMU) consisting of a powered first mirror M1 and a grounded second mirror M2 placed in a homogeneous 3 T magnetic field. The plasma discharge was confined in a beam extending in the direction of the magnetic field, consequently wetting a limited portion of the FMU walls. In the DC-decoupled scheme (without λ/4 filter), this considerably influenced the self-bias voltage V
DC that develops on M1. Changing the angle α between M1 normal and magnetic field, modified the plasma wetted wall area A
g and the resulting V
DC varied by over two orders of magnitude. Plasma exposure experiments were also done in the DC-coupled scheme (with λ/4 filter), wherein the angle and wetted surface determined the area of wall sputtered. Increasing α led to an increase in the sputtered wall area A
g, and consequently the wall deposition on grounded M2. However, in all the cases M1 was entirely clean with the exception of edge deposits in some. In contrast, both M1 and M2 are coated with wall deposits in the absence of a magnetic field and a similar plasma exposure. The results show that plasma cleaning with λ/4 filter in a 3 T magnetic field at ITER could potentially prevent the parasitic wall deposition on FMs. The results also highlight the importance of FM orientation in the magnetic field and the wetted area in the plasma cleaning in both the DC-coupled and decoupled schemes within the ITER diagnostic systems.
Drug-Loaded IRONSperm clusters: modeling, wireless actuation, and ultrasound imaging
Authors: Middelhoek Kaz I N A , Magdanz Veronika , Abelmann Leon , Khalil Islam S M 
Published: August 2022

Abstract
Individual biohybrid microrobots have the potential to perform biomedical in vivo tasks such as remote-controlled drug and cell delivery and minimally invasive surgery. This work demonstrates the formation of biohybrid sperm-templated clusters under the influence of an external magnetic field and essential functionalities for wireless actuation and drug delivery. Ferromagnetic nanoparticles are electrostatically assembled around dead sperm cells, and the resulting nanoparticle-coated cells are magnetically assembled into three-dimensional biohybrid clusters. The aim of this clustering is threefold: First, to enable rolling locomotion on a nearby solid boundary using a rotating magnetic field; second, to allow for noninvasive localization; third, to load the cells inside the cluster with drugs for targeted therapy. A magneto-hydrodynamic model captures the rotational response of the clusters in a viscous fluid, and predicts an upper bound for their step-out frequency, which is independent of their volume or aspect ratio. Below the step-out frequency, the rolling velocity of the clusters increases nonlinearly with their perimeter and actuation frequency. During rolling locomotion, the clusters are localized using ultrasound images at a relatively large distance, which makes these biohybrid clusters promising for deep-tissue applications. Finally, we show that the estimated drug load scales with the number of cells in the cluster and can be retained for more than 10 h. The aggregation of microrobots enables them to collectively roll in a predictable way in response to an external rotating magnetic field, and enhances ultrasound detectability and drug loading capacity compared to the individual microrobots. The favorable features of biohybrid microrobot clusters place emphasis on the importance of the investigation and development of collective microrobots and their potential for in vivo applications.