bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions
Authors: Kassa Eszter , Jamshidi Sara , Mihalič Filip , Simonetti Leandro , Kliche Johanna , Jemth Per , Bergström Lind Sara , Ivarsson Ylva 
Published: October 2022

AbstractLow affinity and transient protein-protein interactions, such as short linear motif (SLiM)-based interactions, require dedicated experimental tools for discovery and validation. Here, we evaluated and compared biotinylated peptide pulldown and protein interaction screen on peptide matrix (PRISMA) coupled to mass-spectrometry (MS) using a set of peptides containing interaction motifs. Eight different peptide sequences that engage in interactions with three distinct protein domains (KEAP1 Kelch, MDM2 SWIB, and TSG101 UEV) with a wide range of affinities were tested. We found that peptide pulldown can be an effective approach for SLiM validation, however, parameters such as protein abundance and competitive interactions can prevent the capture of known interactors. The use of tandem peptide repeats improved the capture and preservation of some interactions. When testing PRISMA, it failed to provide comparable results for a model peptide that successfully pulled down a known interactor using biotinylated peptide pulldown. Overall, in our hands, we find that albeit more laborious, biotin-peptide pulldown was more successful in terms of validation of known interactions. Our results highlight that the tested affinity-capture MS-based methods for validation of SLiM-based interactions from cell lysates are suboptimal, and we identified parameters for consideration for method development.
The N-terminal executioner domains of NLR immune receptors are functionally conserved across major plant lineages
Authors: Chia Khong-Sam , Kourelis Jiorgos , Vickers Martin , Sakai Toshiyuki , Kamoun Sophien , Carella Philip 
Published: October 2022

ABSTRACTNucleotide-binding domain and leucine-rich repeat (NLR) proteins are a prominent class of intracellular immune receptors that are present across diverse plant lineages. However, our understanding of plant NLR structure and function is limited to the evolutionarily young flowering plant clade (angiosperms). Here, we describe an extended spectrum of NLR diversity across major plant lineages and demonstrate functional conservation of N-terminal ‘executioner’ domains that trigger immune responses. We show that broadly distributed CC (coiled-coil) and TIR (toll/interleukin-1 receptor) domains retain executioner function through trans-lineage activation of immune-related cell death in the model angiospermNicotiana benthamiana. Further examination of a CC subfamily specific to non-flowering lineages uncovered an essential N-terminal MAEPL motif with functional similarity to resistosome-forming CC-NLRs. Ectopic activation of the MAEPL-type CC in the divergent liverwortMarchantia polymorphaled to profound growth inhibition, defense gene activation, and signatures of cell death resembling CC activity in flowering plants. Moreover, comparative macroevolutionary transcriptomics inMarchantiaandNicotianaidentified conserved CC responsive genes, providing further insight into the core aspects of CC function shared between flowering and non-flowering plants. Our findings highlight the need to understand NLR structure and function across the full spectrum of plant diversity.
Raw milk kefir: microbiota, bioactive peptides, and immune modulation
Authors: Baars Ton , van Esch Betty , van Ooijen Luuk , Zhang Zuomin , Dekker Pieter , Boeren Sjef , Diks Mara , Garssen Johan , Hettinga Kasper , Kort Remco 
Published: October 2022

AbstractThis study aims to characterize the microbial and peptidomic composition of raw milk kefir, and to address the potential anti-allergic effects of raw milk kefir using validated research models for food allergy. Raw milk kefir was produced after incubation with a defined freeze-dried starter culture. Kefir was sampled during fermentation at seven-time intervals. For comparison, kefir was also prepared from boiled milk (100 °C; 5 sec). Peptide compositions were determined for the raw and heated milk, plus kefir end products made from these milks. In a murine food allergy model, the two kefir end products were investigated on their allergy modulating effects. In both kefirs, we identified amplicon sequence variants identical to those in the starter, matching the bacteriaLactococcus lactis, Streptococcus thermophilus, Leuconostocand the yeastDebaromyces. In raw milk kefir, additional sequence variants ofLactococcus lactisand the yeastsPichiaandGalactomycescould be identified, which were absent in heated milk kefir. Analysis of peptide compositions in kefirs indicated that the number and intensity of peptides drastically increased after fermentation. Heating of milk negatively affected the diversity of the peptide composition in kefir. Only raw milk kefir suppressed the acute allergic skin response to the food allergen ovalbumin in sensitised mice. These effects coincided with differences in the T-cell compartment, with lower percentages of activated Th1 cells and IFNg production after treatment with kefir made from heated milk. The results of this study indicate specific properties of raw milk kefir that may contribute to its additional health benefits.
Synchrotron Mössbauer source: trade-off between intensity and linewidth
Authors: Yaroslavtsev Sergey , Chumakov Aleksandr I. 
Published: October 2022

A synchrotron Mössbauer source (SMS) enables conventional (energy-domain) Mössbauer spectroscopy at synchrotron radiation facilities. In comparison with radioactive sources, SMS provides a beam of several micrometres in size, permitting studies of extremely small samples. The SMS linewidth can be narrowed at the expense of its intensity by varying the angular position and temperature of the key element of the SMS – an iron borate 57FeBO3 crystal. Here, in order to optimize the SMS performance, the angular and temperature dependencies of the SMS parameters have been studied and the optimal angular position and temperature of the crystal have been determined for highest intensity at specified source width. The results show that, when accepting broadening of the source width up to ∼6 natural widths, the intensity of the SMS at the European Synchrotron reaches more than 105 γ-quanta s−1. In the opposite extreme, the width of the source approaches the natural width with intensity decreasing to about 103 γ-quanta s−1. These changes of intensity up to two orders of magnitude take place over a temperature range of about 0.5°C. For all temperature and angular conditions, the instrumental function of the source was derived; we also analyzed the modification of its shape when passing from the `low-width' to `high-intensity' extremes of SMS operation. Finally, we estimated the influence of the temperature instability and mosaicity of the iron borate crystal on the SMS performance.
Speckle contrast of interfering fluorescence X-rays
Authors: Trost Fabian , Ayyer Kartik , Oberthuer Dominik , Yefanov Oleksandr , Bajt Saša , Caleman Carl , Weimer Agnes , Feld Artur , Weller Horst , Boutet Sébastien , Koglin Jason , Timneanu Nicusor , von Zanthier Joachim , Röhlsberger Ralf , Chapman Henry N. 
Published: October 2022

With the development of X-ray free-electron lasers (XFELs), producing pulses of femtosecond durations comparable with the coherence times of X-ray fluorescence, it has become possible to observe intensity–intensity correlations due to the interference of emission from independent atoms. This has been used to compare durations of X-ray pulses and to measure the size of a focusedX-ray beam, for example. Here it is shown that it is also possible to observe the interference of fluorescence photons through the measurement of the speckle contrast of angle-resolved fluorescence patterns. Speckle contrast is often used as a measure of the degree of coherence of the incident beam or the fluctuations of the illuminated sample as determined from X-ray diffraction patterns formed by elastic scattering, rather than from fluorescence patterns as addressed here. Commonly used approaches to estimate speckle contrast were found to suffer when applied to XFEL-generated fluorescence patterns due to low photon counts and a significant variation of the excitation pulse energy from shot to shot. A new method to reliably estimate speckle contrast under such conditions, using a weighting scheme, is introduced. The method is demonstrated by comparing the speckle contrast of fluorescence observed with pulses of 3 fs to 15 fs duration.
Measuring the impact of surface ozone on rice production in China: a normalized profit function approach
Authors: Ge Yang , Yang Yongbing , Yi Fujin , Hu Hao , Xiong XiaoBai 
Published: October 2022

PurposeThe purpose of this study is to investigate the impacts of surface ozone pollution on rice profit, output and variable inputs in China.Design/methodology/approachThis study estimates the rice profit function using county-level rice production data and ozone monitoring data in 2014 and 2015 to capture the impact of ozone pollution on rice profit. Then, it uses dual approach to identify the impacts of ozone on the supply of rice and the demand for variable inputs. The ozone concentration data are obtained from 1,412 monitoring stations established by the National Environmental Monitoring Centre of China.FindingsThe results show that surface ozone would significantly reduce rice profits; a 1% increase in (the daily average ozone concentration from 9 am to 4 pm) leads to a 0.1% decrease in profits. In addition, ozone has a negative impact on the levels of inputs and the supply of rice, and the elasticities of rice output, fertilizer input and labour input with respect to are −0.87, −0.86 and −0.78%, respectively. These results suggest that ozone pollution affects rice production via two channels: the direct damage on rice growth and the indirect negative impact of reducing variable inputs.Originality/valueThis study estimates the impacts of surface ozone pollution on rice profit and output, and quantifies its influence on variable inputs in China, which provides a better understanding of farmers' adaptation behaviour.
Can SMEs' quality management promote supply chain financing performance? An explanation based on signalling theory
Authors: Lu Qiang , Yang Yudong , Yu Miao 
Published: October 2022

PurposeThe purpose of this study is to examine how the quality management of small and medium-sized enterprises (SMEs) impacts their supply chain financing performance (SCFP). This study also investigates the mediating roles of organisational dependence between quality management and the SCFP of SMEs, as well as the moderating role of environmental dynamics.Design/methodology/approachQuestionnaires were administered to 248 financial managers responsible for supply chain finance (SCF) in SMEs in China. Data analysis techniques used include necessary condition analysis and multiple regression analysis.FindingsResearch findings show that, in SCF, the quality management of SMEs positively predicts their SCFP through the mediation of the organisational dependence of the focal enterprises in the supply chain network. Environmental dynamics are also found to moderate the relationship between quality management and SCFP through the organisational dependence of capital providers.Originality/valueTo the best of our knowledge, this is the first study to explore the relationships between SMEs' quality management and their SCFP. Also, this study provides a new theoretical lens through which to study SCF by introducing signalling theory.
Evaluation of a coach door under a frontal impact
Authors: Lopes Rogério , Barros Francisco , Melo Francisco Q. de , Ramos Nuno V. , Cunha Rafael , Maia Ricardo , Rodrigues Rui , Parente M.P.L. , Moreira P.M.G. 
Published: October 2022

PurposeThe vehicle´s body front pillar should absorb most of the striker kinetic energy, while only a fraction of that is absorbed by the door structure. This study aims to discuss the aforementioned issue. In this test the striker is a virtual entity. Six uniaxial strain gauges are installed throughout the door. Additionally, contactless 3D digital image correlation (DIC) allows to assess the major door panel’s continuous deformation and strain fields.Design/methodology/approachA coach is a large and heavy long-distance passenger transport vehicle. Their structural certification, classifies coaches as M3 Class III vehicles. New coach structures’ designs need analyses of each sub-system for critical pre-validation of the entire structure, aiming driver and passenger carrier safety. Also, a thorough examination due to increased travel speed is needed.FindingsExperimental pseudo-dynamic (PSD) results were compared and validated using finite element method (FEM) with two pieces of distinct FEM software (Abaqus® and PamCrash®). The time dependent solution was carried out by explicit techniques. Results by FEM and PSD test showed good agreement, evidencing the reliability of the tools selected. Results by PamCrash® were closer to the experimental data.Practical implicationsR-29 is truck-only regulation, however can be adapted to coaches in case of a frontal collision. The present work focuses on the impact behavior of the passenger front door subsystem.Originality/valueAs a first validation the entire structure, the behavior of a vehicle door, under in-plane impacts was studied. The corresponding deformation energy absorbed by the frontal passenger coach door under virtual impacts of a swinging striker was assessed using a PSD approach.
Earnings management and corporate efficiency pre- and post-IFRS: evidence from Taiwanese listed electronics corporations
Authors: Wang Wei-Kang , Lu Wen-Min , Ting Irene Wei Kiong , Siao Wun-Ya 
Published: October 2022

PurposeThis study aims to examine the relationships among International Financial Reporting Standards (IFRS) adoption, earnings management, and corporate efficiency.Design/methodology/approachFirst, the authors employ the epsilon-based measure (EBM) of the data envelopment analysis to measure the corporate performance of the Taiwanese electronics industry from 2011 to 2014. Second, the authors regress the IFRS adoption and earnings management on corporate efficiency.FindingsThe findings show that the corporate efficiency deteriorated after IFRS adoption. Although the regression analysis shows that the relationship between earnings management and corporate efficiency is significantly positive, the authors find that IFRS adoption is effective in unveiling earnings management. Moreover, IFRS adoption moderates the impact earnings management and corporate efficiency.Research limitations/implicationsThis study provides reference for decision-makers in the application of accounting principles and in the understanding of the IFRS impact adoption.Practical implicationsIFRS adoption can either facilitate or limit the earnings management that would affect corporate efficiency significantly and help the electronics industry as well as investors to know the changes in accounting principles and their effects on corporate efficiency.Originality/valueThe authors use the EBM of efficiency model to measure corporate efficiency and employ the modified Jones model to measure earnings management.
The business models of NFTs and fan tokens and how they build trust
Authors: Zarifis Alex , Cheng Xusen 
Published: October 2022

PurposeThe sale of NFTs and the interest in them has “exploded” recently. While there is a lot of information on them, it is not clear what final form they will take. There are several reasons to be uneasy, such as the prevalence of scams, but there are also reasons for optimism and confidence in NFTs. First, they solve the problem of how to own digital assets. Additionally, some of the more reliable and proven cryptoasset exchanges are offering them. However, this innovation will have difficulties reaching a wider audience until more clarity is achieved on two main issues. Therefore, this study aims to clarify what the NFT business models are, and how do they build trust.Design/methodology/approachThis research attempts to identify the NFT business models with case study analysis in three stages. The first stage is a focus group, followed by 16 short case vignettes and finally four longer case studies.FindingsThe findings show that there are four NFT business models: (1) NFT creator; (2) NFT marketplace, selling creators’ NFTs; (3) company offering their own NFT (fan token) and (4) computer game with NFT sales.Originality/valueThis research brings the literature on business models and NFTs together to offer clarity on the proven NFT business models.
Creation of shared value in cooperatives: informal institutions’ perspective of small-sized banana growers from Colombia
Authors: Maestre Matos Marcela , Lombana-Coy Jahir , Mesías Francisco J. 
Published: October 2022

PurposeThis study aims to identify informal institutions for bottom-of-the-pyramid (BoP) business models in the agricultural sector through the case study of banana growers’ cooperatives.Design/methodology/approachA case study of six banana cooperatives from Colombia was conducted. The research followed a mixed design, using both qualitative and quantitative data and the application of structural equations.FindingsThis study shows that social capital, networking and alliances are essential in BoP businesses.Originality/valueAuthors defined a model of informal institutional factors for the generation of economic and social value in inclusive business, using the new institutional theory and the conceptual development of BoP in agri-business.
Effects of online brand community rituals on customer citizenship behavior: exploring the sequential mediation mechanism
Authors: Yang Shuang , Tang Jiarong , Cai Jian , Guo Gongxing 
Published: October 2022

Purpose
Few extant studies have focused on digital rituals and investigated the relationship between them and customer citizenship behavior in the context of online brand communities (OBCs). This study aims to examine the sequential mediation mechanism of emotional energy and spiritual brand identification under interaction ritual theory and identifies membership prototypicality as the moderator.

Design/methodology/approach
An online investigation of 515 OBC users was conducted to gather data, and structural equation modeling was applied to test the hypotheses.

Findings
The empirical results revealed that OBC rituals were positively related to customer citizenship behavior. Emotional energy and spiritual brand identification could play mediating roles in the relationship between OBC rituals and customer citizenship behavior. Furthermore, there existed a sequential mediation mechanism with emotional energy as the first mediator and spiritual brand identification as the second. The effect of OBC rituals on emotional energy was more significant for peripheral members than prototypical members.

Practical implications
Managers of OBCs should conduct various ritualistic strategies to stimulate users to perform customer citizenship behaviors. Discrete ritualized activities should be intended for members of different prototypicalities.

Originality/value
This study provides a profound insight on how OBC rituals foster customer citizenship behavior and is among the first to explore such a relationship. It also investigates the sequential mediation mechanism, thus broadening the research on the influencing processes of OBC rituals on customer citizenship behavior.
Adaptation of autism spectrum screening questionnaire (ASSQ) for use in Georgian school settings
Authors: Zirakashvili Medea , Gabunia Maia , Mebonia Nana , Mikiashvili Tamar , Lomidze Giorgi , Bishop Somer , Leventhal Bennett , Kim Young Shin 
Published: October 2022

Purpose
Even though 95% of children with neurodevelopmental disorders, including autism spectrum disorder (ASD), live in low- and middle-income countries, there is a dearth of studies from these countries, including the Republic of Georgia. Several ASD screening tools are available, but few are validated for use in Georgian or other smaller countries. This study aims to adapt and validate the autism spectrum screening questionnaire (ASSQ) for use in Georgia.

Design/methodology/approach
The ASSQ was administered for all third-grade students in 402 schools in the five main Georgian cities, n = 27,336. Prior to use, the 27-item ASSQ was translated, back-translated and adapted for use in Georgia. A total of 16,556 students (approximately 61%) were assessed by a parent and/or teacher. Optimal cutoff scores were estimated. Randomly chosen children who screened positive (n = 173) and negative (n = 127) were offered comprehensive assessment using standardized diagnostic procedures.

Findings
Data from 15,510 parents- and 13,517 teachers-administered ASSQ revealed statistically significant differences in median and cutoff scores between parents and teachers: 7 versus 4 and 9 versus 6, respectively. Cutoff score = 14, on either parent or teacher ASSQ, had sensitivity of 0.94, indicating that it can be used in school settings.

Originality/value
The Georgian adaptation of the ASSQ creates opportunity for further ASD research, while also providing a valid screening tool for clinicians. Data from Georgia will add to the growing understanding of the broader ASD phenotype.
Epidemiological survey of patients with psoriatic arthritis in the Japanese Society for Psoriasis Research from 2017 to 2020
Authors: Kamiya Koji , Ohtsuki Mamitaro 
Published: October 2022

AbstractThe Japanese Society for Psoriasis Research (JSPR) conducted annual epidemiological surveys of patients with psoriatic arthritis (PsA). This study aimed to analyze a recent epidemiological survey of enrolled PsA patients in the JSPR from 2017 to 2020. A total of 1641 patients (1032 men [62.9%] and 609 women [37.1%]) were enrolled from 131 medical institutions. The mean ± standard deviation age of the patients was 51.4 ± 13.6 years and those at the onset of skin lesions and joint symptoms were 39.2 ± 15.8 and 47.9 ± 14.0 years, respectively. According to the Moll and Wright criteria, distal, oligoarticular, polyarticular, arthritis mutilans, and axial or spondyloarthritis types were 27.2%, 29.9%, 18.6%, 0.4%, and 6.6%, respectively. Approximately 56.3% of the patients had past history and comorbidities, such as hypertension (35.9%), dyslipidemia (20.7%), diabetes mellitus (19.2%), hyperuricemia (13.5%), cardiovascular disease (4.1%), and cerebrovascular disease (3.9%). Regarding systemic therapy, 55.9% and 45.5% of the patients were treated with oral medications and biologics, respectively. The most common oral medication was methotrexate (39.1%), followed by apremilast (27.4%). Non‐steroidal anti‐inflammatory drugs were also used in many patients (40.3%). Among the biologics, the most common was adalimumab (30.1%), followed by secukinumab (20.9%) and ixekizumab (17.0%). This survey shows the recent perspective of PsA in the Japanese society, which will lead to a better understanding of this disease, including patient characteristics, comorbidities, and treatment trends.