bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of Sonoelastography for Predicting Malignancy in soft Tissue
Authors: Bodard Sylvain , Lassalle Louis , Larousserie Frédérique , Guinebert Sylvain , Hacquart Thomas , Campagna Raphael , Biau David , Regnard Noreddine , Feydy Antoine 
Published: July 2022

Abstract
Background: Separating benign from malignant soft-tissue masses often requires a biopsy. The objective of this study was to assess whether shear-wave elastography (SWE) helped to separate benign from malignant soft-tissue masses. Methods: In 2015–2016, we prospectively included patients with soft-tissue masses deemed by our multidisciplinary sarcoma board to require a diagnostic biopsy. All patients underwent ultrasonography (US) followed by SWE to measure elasticity. We compared benign and malignant tumors, overall and after separating tumors with vs. without a fatty component. The biopsy findings, and surgical-specimen histology when available, served as the reference standard. Results: We included 136 patients, 99 with non-fatty and 37 with fatty soft-tissue masses. Mean elasticity and T/F values were significantly lower for the benign than the malignant soft-tissue masses in the overall cohort (30.9 vs. 50.0 kPa, P=0.03; and 2.55 vs. 4.30, P=0.046) and in the non-fatty subgroup (37.8±31.9 vs. 58.9±39.1 kPa, P=0.049 and 2.89±5.25 vs. 5.07±5.41, P=0.046). Data for fatty tumors were non relevant due to lack of conclusive results. By receiver operating characteristics curve analysis, a T/F cutoff of 3.5 had 46% sensitivity and 84% specificity for separating benign and malignant soft-tissue masses. Conclusions: SWE had good specificity for separating benign from malignant soft-tissue masses.
Identification of the cuproptosis related prognostic gene signature and the associated regulation axis in clear cell renal cell carcinoma
Authors: Guo Lingyu , An Tian , Wan Ziyan , Huang Zhixin , Chong Tie 
Published: July 2022

Abstract
Background
The most common type of renal cell carcinoma is clear cell renal cell carcinoma (ccRCC), which has a poor prognosis. In addition to regulating tumor cells' growth and metabolism, cuproptosis can also affect patients' prognosis. However, the function of cuproptosis-related genes(CRGs) in ccRCC remains unclear.
Methods
Comprehensive bioinformatics analysis was used to evaluate CRGs expression level, prognostic value and relationship with tumor immune infiltration. Furthermore, we constructed prognostic gene models and ceRNA networks based on bioinformatics analysis.
Results
In ccRCC, we found a total of 16 CRGs up-regulation or down-regulation. We also focused on the mutation of CRGs in ccRCC. Based on prognosis analysis, these genes were associated with ccRCC prognosis. We subsequently constructed a prognostic CRGs model to predict the prognosis of ccRCC patients. Immunocorrelation studies revealed the correlation between CRGs and tumor immune infiltration. The lncRNA OIP5-AS1/miR-222-3p/DBT regulatory axis was constructed in ccRCC. We also verified the expression of DBT and lncRNA OIP5-AS1 in ccRCC cell lines via the qRT-PCR method.
Conclusions
Through comprehensive bioinformatics analysis, we analyzed the expression and prognostic value of CRGs in ccRCC, and identified the prognostic CRGs signature of ccRCC patients, including NLRP3, FDX1, LIPT2, GLS, CDKN2A, DBT and GCSH. Our follow-up study also found an lncRNA OIP5-AS1/ Mir-222-3p /DBT regulatory axis, which may explain the internal mechanism of CRGs involved in regulating the progression of ccRCC.
Covid-19: Relative risks of non-vaccinated to vaccinated individuals
Authors: Barbieri Davide , Melegari Gabriele , Bertellini Elisabetta , Gaspari Arianna , Halasz Geza 
Published: July 2022

Abstract
ObjectivesThe aim of this study was to evaluate and quantify the relative risk of hospital admission and death because of Sars-Cov2 infection between non-vaccinated and vaccinated individuals in Italy.MethodsData about vaccinated and non-vaccinated people, infections, hospital admissions, intensive care units and fatalities were extracted from the bulletin published by the Italian National Health Institute (Istituto Superiore di Sanità) on the 24th of December 2021. Likelihood ratios of hospital admission, intensive care and death were calculated between non-vaccinated and vaccinated people for each of the observed patient outcomes, in order to quantify the relative risk in the two sub-populations.ResultsNon-vaccinated people had a 3.1 time higher risk of becoming infected compared to vaccinated individuals. Non-vaccinated individuals had a 5.1 times higher risk of being admitted to hospital and a 10.4 times higher risk of becoming critical compared to vaccinated individuals. Further, non-vaccinated people had a 4.3 times higher risk to die compared to vaccinated individuals.ConclusionsThe relative risk of infection, hospital and intensive care admission is progressively higher in non-vaccinated compared to vaccinated individuals in case of infection by Sars-Cov2, as the condition worsens. Still, the relative risk does not increase further when the outcome is death, possibly because non-vaccinated individuals are younger and healthier of those who decided to take the vaccine. Individual conditions may play a more relevant role than the vaccine when the illness becomes critical.
Full Genome Characterization of a putative novel serotype of Yonaguni orbivirus isolated from cattle in Yunnan Province, China
Authors: Yang Zhengxing , He Yuwen , Meng Jinxin , Li Nan , Wang Jinglin 
Published: July 2022

Abstract
In July 2019, a virus strain (JH2019C603) was isolated from a sentinel cattle in Jinghong city, the subtropical region of Yunnan Province, China. The virus replicated and caused cytopathological effects (CPE) in both Aedes albopictus (C6/36) and Baby Hamster Syrian Kidney (BHK-21) cells. Agarose gel electrophoresis (AGE) analysis revealed the viral genome consisted of 10 segments of double-stranded RNA, with a ‘1-2-2-1-1-1-1-1’ migration pattern. Complete genome sequences of the JH2019C603 virus were determined by full-length amplification of cDNAs. Phylogenetic analysis based on the amino acid (aa) sequences of RNA-dependent RNA Polymerase (Pol), Major subcore (T2), Major core-surface (T13), showed that JH2019C603 clustered with Yonaguni orbivirus (YONOV) from Japan, and the aa identity with those of YONOV was 97.7% (Pol), 99.0% (T2) and 98.5% (T13), respectively. However, phylogenetic analysis based on the aa sequences of OC1 and OC2 proteins, showed that JH2019C603 formed an independent branch in phylogenetic tree, and the aa identity with YONOV was only 55.4% (OC1) and 80.8% (OC2), respectively. Compared with the prototype of YONOV, a notable sequence deletion was observed in 3’-NCRs of NS1, and the NS1 of JH2019C603 was encoded within Seg-7, unlike the NS1 of YONOV, which is encoded in Seg-6. These indicated that JH2019C603 belongs to the YONOV, but might be a novel serotype or a highly variant strain of YONOV. This is first time that YONOV has been isolated in China, providing new insights into the genetic diversity and geographic distribution of this virus species.
DNA methylation regulator-based modification patterns define distinct immune phenotypes and immunotherapy responses in colorectal cancer
Authors: Yang Chao , Cao Fengyu , Cheng Hua , Zheng Yongbin 
Published: July 2022

Abstract
Background: A major challenge in immunotherapy is identifying populations that can benefit from treatment. DNA methylation is one of the most extensively studied epigenetic regulation mechanisms and reportedly involved in immune regulation and anticancer immune responses. How the integrated DNA methylation modification pattern affects the tumor microenvironment (TME) and the response to immunotherapy is poorly understood, however. Methods: In this study, samples from 1,691 colorectal cancer (CRC) patients recruited from five independent cohorts were used to comprehensively evaluate DNA methylation regulator patterns and TME characteristics. DNA methylation regulator patterns were determined using unsupervised hierarchical clustering analysis based on 20 DNA methylation regulators. The CIBERSORT and single-sample gene set enrichment analysis algorithms were used to respectively assess tumor-infiltrating immune cell relative abundance and immune-related pathways in each sample. A principal component analysis algorithm–based DNA methylation scoring scheme (DM_Score) was devised to quantify DNA methylation levels in candidates. The predictive value of the DM_Score for immunotherapy was evaluated in two cohorts. The association between the DM_Score and TME was assessed in an external cohort from our center.Results: Three DNA methylation regulator patterns associated with various clinical outcomes and TME characteristics were identified, consistent with classical immune phenotypes (immune-desert, immune-inflamed, and immune-excluded). The DM_Score established based on 270 DNA methylation-related signature genes strongly predicted unfavorable CRC prognoses associated with various mutations and TME landscapes. In addition, the DM_Score was positively correlated with many recognized biomarkers of immunotherapy response, including tumor mutational burden, tumor immune dysfunction and exclusion, immunophenoscore, PD-L1 mRNA, and microsatellite instability. In the two immunotherapy cohorts, a significant survival advantage was observed in patients with a high DM_Score after anti-PD-1/PD-L1 therapy.Conclusions: We present the first comprehensive analysis of cross-talk between DNA methylation modification patterns and TME characteristics in CRC. Quantitative evaluation of DNA methylation levels in individual tumors can aid in guiding more-precise immunotherapy strategies.
Experimental study on freeze-thaw damage characteristics of coal samples of different moisture contents in liquid nitrogen
Authors: Wang Xiaoqi , Qi Xiaohan , Ma Heng , Gao Ke , Li Shengnan 
Published: July 2022

Abstract
In this study, the surface crack-propagation law and pore damage characteristics of coal samples of different water contents after they undergo leaching in liquid nitrogen are investigated using a 4K scientific -research camera, HC-U7 non-metal ultrasonic detector, nuclear magnetic resonance testing technology, and self-made multi-functional three axial fluid-solid coupling test system. Experimental investigations are conducted on coal samples of different water contents before and after they undergo liquid-nitrogen freezing and thawing in order to determine the propagation law of surface fissures, the development law of internal micro-fissures, the development process of internal pores, the change law of the pore-size distribution, and the law of coal-sample deformation and gas seepage during the stress process. The test results show that, with the increase in water content in the liquid-nitrogen leaching process, the frost heave force on the coal surface increases, and the greater the increase ratio of the coal porosity, the faster is the development of micro-cracks and pores. Under the action of liquid nitrogen, the number of micro-pores, meso-pores, and macro-pores in the coal sample increased, and with the formation of new cracks and the connection of the original cracks, liquid-nitrogen freezing and thawing can promote the development of the pore structure in the coal body. The permeability changes of coal samples of different water contents during unloading failure exhibit obvious stage characteristics. The above results demonstrate that the moisture content of coal has a significant effect on the development of surface cracks and pore-damage characteristics of coal after liquid-nitrogen freezing and thawing, and there is a positive correlation between the surface crack expansion and internal damage of the coal samples of different moisture contents leached in liquid nitrogen.
Synthesis, biological evaluation and molecular docking studies of a new series of bis-chalcones
Authors: Khazaei-poul Zahra , Mahmoodi Nosrat Ollah , Nahzomi Hossein Taherpour 
Published: July 2022

Abstract
A convenient and efficient reaction for the synthesis of several new bis-hydroxy derivatives of chalcones 4a-h was accomplished via the two-directional Claisen Schmidt condensation of different ketones 3a-e with premade benza-ldehyde 2 under alkaline conditions. Products were obtained with reasonable yields and with excellent purity. The in vitro antioxidant activity of bis-hydroxychalcones were assessed by DPPH radical scavenging activity in comparison to ascorbic acid and products showed good antioxidant activity. To determine the possible binding mechanism with the active site of Tubulin, docking analyses were performed. According to the docking results, ompound s 4a-b showed the best binding affinity of -9.13 and -9.11 kcal/mol for Tubulin, respectively.
Utilization of Chitosan and Polyacrylamide Gel to Remove Direct Dyes from Wastewater
Authors: ElZahar Medhat M. H. 
Published: July 2022

Abstract
The adsorption of direct blue 78 dye with a commercial (DB78) from synthetic wastewater using a pure chitosan powder and chitosan micro-beads has been studied. The ability of chitosan as a bio-adsorbent to remove dyes from textile wastewater effluent has been investigated in detail. This study aims to achieve the highest removal efficiency with a lower contact and sedimentation time of direct blue dye using chitosan particles enhanced with polyacrylamide polymer. A series of batch experiments were conducted in terms of adsorbent dose, contact time, and pH. The chitosan particles were characterized by physicochemical analysis namely; FTIR and Zeta potential analysis. It was found that the net surface charge was + 6.52 mv and + 28.77 mv respectively for pure chitosan powder and chitosan micro-beads. The adsorption tests showed that the removal efficiency increases with increasing adsorbent dosage and contact time. Removal efficiency decreases at the high initial concentration of direct blue 78 dye. The maximum capacities for removal of dyes were achieved up to 94.2% with an initial concentration of 50 mg/l and pure chitosan dose of 4.5 g/L and an optimal contact time of 60 minutes. Furthermore, the sedimentation time for chitosan powder decreases from 8 hours to 10 minutes by using a polyacrylamide gel dose of 45 mL/L of synthetic wastewater. Studies have shown that equilibrium data followed Freundlich and Langmuir isotherm closely, but the latter isotherm better suited the results with R2 = 0.997. These results revealed that the use of pure chitosan powder enhanced with adding polyacrylamide gel is an effective adsorbent for the removal of direct blue 78 dye. The removal efficiency is considered high with a low adsorbent dose compared to using pure chitosan micro-beads.
Determination of Immunostimulatory Efficacy of Benzimidazole Derivatives Against Leishmania infantum
Authors: Kelleci Kübra 
Published: July 2022

Abstract
Leishmaniasis is a parasitic disease affecting more than 12 million people worldwide. Although the use of chemotherapeutic drugs is among the current treatment methods, it is emphasized that the limitations of the drugs, their disadvantages such as insufficient safety and efficacy, the disease being endemic, and the need to develop drugs for this disease. In this study, in vitro anti-leishmanial activities of two benzimidazole derivatives (B1 and B2) were tested against L. infantum promastigote and amastigote for the first time. In our study, the anti-leishmanial effects of B1 and B2 on both amastigote and promastigote forms of Leishmania infantum were investigated using J774 macrophage cells. It was found that the tested compounds were not toxic to the host cell. In addition, when compared to the drug miltefosine, which is used worldwide in the treatment of leishmaniasis, it has been determined that it has a serious inhibitory effect by reducing the proliferation of promastigotes and the metabolic activities of amastigotes, and it acts at lower concentrations than miltefosine. We found that benzimidazolium derivatives (B1 and B2) used for the first time in this study were more effective on both forms of L. infantum. The obtained results showed that benzimidazolium derivatives have high anti-leishmanial potential against L. infantum, which is the cause of visceral leishmaniasis (VL), which is known to be the deadly form of leishmaniasis. It has been shown that the obtained results will help the development of safe, stable, and effective anti-leishmanial drug formulations against VL.
Preventive treatment can reverse cognitive impairment in chronic migraine
Authors: González-Mingot Cristina , Gil-Sánchez Anna , Canudes-Solans Marc , Peralta-Moncusi Silvia , Solana-Moga Maria José , Brieva-Ruiz Luis 
Published: July 2022

Abstract
Objective. To study the impact of chronic migraine (CM) on the cognition and quality of life (QoL) of patients in the interictal period, and to analyze the degree of reversibility of any observed alterations following the use of preventive treatment.Background. CM is a highly disabling disease, and migraineurs often have associated comorbidities, such as subjective memory problems, that are involved in the development of cognitive impairment.Methods. The cognition and QoL of 50 control subjects and 46 patients with CM were assessed using a battery of tests, prior to the use of preventive treatment based on botulinum toxin or oral drugs and after 3 months of this treatment.Results. Compared with controls, patients with CM had lower scores on the assessment of cognitive performance (Rey-Osterrieth Complex Figure test [ROCF], Trail Making Test [TMT] B) (p < 0.05) and QoL (p < 0.05). Three months after the use of preventive treatment, improvement was observed in all cognitive parameters (p < 0.05) and QoL (p < 0.05), except the ROCF copy task (p = 0.79). No statistically significant differences were observed when these outcomes were compared based on treatment.Conclusions. This study confirms poor cognitive performance that is not explained by migraine pain itself, as it occurs in the interictal period, irrespective of the patients’ emotional status. Our findings show that these effects are reversible in some cases with preventive treatment of CM, reaffirming the important impact of this condition on the QoL of these patients, and the need to establish preventive treatment guidelines.
Biological Isolation and Characterization of Catharanthus roseus (L.) G. Don Methanolic Leaf Extracts and their Assessment of Antimicrobial, Cytotoxic and Apoptotic Activities
Authors: Rajashekara Somashekara , Reena Dondapati , Mainavi Mullahalli VenkataramaReddy , Sand Locheruvapalli Srinivasa , Baro Utpal 
Published: July 2022

Abstract
Background: Biological synthesis of natural products from the plants has made us an inspiring methodology in the arena of science and biotechnology. Methods: The methanolic leaf extracts of Catharanthus roseus (L.) G. Don plants (CrPLE) were isolated/extracted and characterized utilizing phytochemicals estimation, Thin-layer chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) analysis; and further in understanding the biomedical uses of CrPLE. The evaluation of the seven phytochemicals clearly designates that the presence of secondary metabolites in the CrPLE. Results: The CrPLE (test samples) exhibited the Catechin and Caffeic acid contents of 0.0055 and 0.0149 mg/g respectively. The CrPLE unveiled highest antimicrobial activity and confirmed with mortal effect against the tested microorganisms. A cytotoxicity known against the breast cancer cell lines of exposed that CrPLE as a good anticancer agent and metabolically vigorous cells. Conclusion: Consequently, the biological synthesized methanolic leaf extracts of the C. roseus plants would be appreciated and have the incredible contributions in the field of medicinal applications.
Assessment of Zataria multiflora essential oil- incorporated electrospun polyvinyl alcohol fiber as active packaging
Authors: Moradinezhad Farid , Hedayati Sara , Ansarifar Elham 
Published: July 2022

Abstract
In this study, an active packaging containing Zataria multiflora essential oil (ZMEO), a powerful natural antimicrobial agent encapsulated into polyvinyl alcohol (PVA) fiber by electrospinning is presented. ZMEO was effective on pathogenic bacteria, particularly gram-positive bacteria, fungi and yeasts. Results showed that the scanning electron microscopy (SEM) images of fibers had bead-free and uniform structure. Fourier transform infrared (FTIR) revealed that ZMEO was encapsulated into PVA through a physical process, without chemical interaction between the ingredients. Strawberries treated with PVA/ZMEO significantly (p˂0.05) preserved the anthocyanin, total phenols, antioxidant, soluble solids, titratable acidity, firmness, and color during 15 days of cold storage. According to these findings, electrospinning was an efficient method for encapsulating bioactive compounds. ZMEO loaded into PVA fiber delayed the physiological and biochemical changes of fruits and extended the fruit shelf-life. This study revealed the benefits of incorporating ZMEO into PVA fiber mats, which could lead to new possibilities for active packaging.
Comprehensive chemical profiling of volatile constituents of Angong Niuhuang Pill in vitro and in vivo based on gas chromatography coupled with mass spectrometry
Authors: Jiang Yue , Li Jie , Ding Meng , Guo Zi-Fan , Yang Hua , Li Hui-Jun , Gao Wen , Li Ping 
Published: July 2022

Abstract
Background: Angong Niuhuang Pill (ANP), a renowned precious traditional Chinese medicine prescription, is extensively utilized for the clinical treatment of stroke, meningitis and encephalorrhagia in China. As a classic resuscitation-inducing aromatic prescription, ANP has been investigated for its pharmacological effects in recent years, while the volatile composition in ANP still lacks comprehensive elucidation.Method: To better explore the volatile constituents in ANP, a qualitative analysis method was developed based on the gas chromatography coupled with mass spectrometry. Furthermore, a validated quantitative method was established to determine 21 main compounds in 8 batches of commercially available ANP samples by gas chromatography-tandem mass spectrometry. The quantitative data were successively subjected to Pearson correlation coefficient analysis. Additionally, the absorbed volatile constituents in rat plasma after single oral administration of ANP have also been characterized.Results: A total of 93 volatile constituents including 29 sesquiterpenoids, 28 monoterpenoids, 13 fatty acids and their esters, 7 alkanes, 6 ketones, 3 phenols, 3 aldehydes, 2 benzoate esters, and 2 other types, were preliminarily characterized, which primary originated from Borneolum, Moschus, Curcumae Radix, and Gardeniae Fructus. d-Borneol, isoborneol and muscone were the top three abundant ingredients (>600 μg/g) in 8 batches of ANP samples. Subsequently, the average Pearson correlation coefficient of the contents of 21 analytes was 0.993, inferring the high batch-to-batch similarity among 8 batches. After oral administration of ANP, d-borneol, isoborneol, muscone and camphor were the main volatile constituents absorbed in the rat plasma.Conclusion: This research may be helpful for the comprehensive quality control study of ANP, and provide for guarantee the clinical efficacy of ANP.