bluuebunny/crossref_metadata_2025_split huggingface dataset:
One year of AU Mic with HARPS – II. Stellar activity and star–planet interaction
Authors: Klein Baptiste , Zicher Norbert , Kavanagh Robert D , Nielsen Louise D , Aigrain Suzanne , Vidotto Aline A , Barragán Oscar , Strugarek Antoine , Nicholson Belinda , Donati Jean-François , Bouvier Jérôme 
Published: March 2022

ABSTRACT
We present a spectroscopic analysis of a 1-yr intensive monitoring campaign of the 22-Myr old planet-hosting M dwarf AU Mic using the HARPS spectrograph. In a companion paper, we reported detections of the planet radial velocity (RV) signatures of the two close-in transiting planets of the system, with respective semi-amplitudes of 5.8 ± 2.5 and 8.5 ± 2.5 m s−1 for AU Mic b and AU Mic c. Here, we perform an independent measurement of the RV semi-amplitude of AU Mic c using Doppler imaging to simultaneously model the activity-induced distortions and the planet-induced shifts in the line profiles. The resulting semi-amplitude of 13.3 ± 4.1 m s−1 for AU Mic c reinforces the idea that the planet features a surprisingly large inner density, in tension with current standard models of core accretion. Our brightness maps feature significantly higher spot coverage and lower level of differential rotation than the brightness maps obtained in late 2019 with the SPIRou spectropolarimeter, suggesting that the stellar magnetic activity has evolved dramatically over a ∼1-yr time span. Additionally, we report a 3σ detection of a modulation at 8.33 ± 0.04 d of the He i D3 (5875.62 Å) emission flux, close to the 8.46-d orbital period of AU Mic b. The power of this emission (a few 1017 W) is consistent with 3D magnetohydrodynamical simulations of the interaction between stellar wind and the close-in planet if the latter hosts a magnetic field of ∼10 G. Spectropolarimetric observations of the star are needed to firmly elucidate the origin of the observed chromospheric variability.
Modulation of RNA stability regulates gene expression in two opposite ways: through buffering of RNA levels upon global perturbations and by supporting adapted differential expression
Authors: Faucillion Marie-Line , Johansson Anna-Mia , Larsson Jan 
Published: March 2022

Abstract
The steady state levels of RNAs, often referred to as expression levels, result from a well-balanced combination of RNA transcription and decay. Alterations in RNA levels will therefore result from tight regulation of transcription rates, decay rates or both. Here, we explore the role of RNA stability in achieving balanced gene expression and present genome-wide RNA stabilities in Drosophila melanogaster male and female cells as well as male cells depleted of proteins essential for dosage compensation. We identify two distinct RNA-stability mediated responses involved in regulation of gene expression. The first of these responds to acute and global changes in transcription and thus counteracts potentially harmful gene mis-expression by shifting the RNA stability in the direction opposite to the transcriptional change. The second response enhances inter-individual differential gene expression by adjusting the RNA stability in the same direction as a transcriptional change. Both mechanisms are global, act on housekeeping as well as non-housekeeping genes and were observed in both flies and mammals. Additionally, we show that, in contrast to mammals, modulation of RNA stability does not detectably contribute to dosage compensation of the sex-chromosomes in D. melanogaster.
Intratumoral heterogeneity of MYC drives medulloblastoma metastasis and angiogenesis
Authors: Qin Nan , Paisana Eunice , Langini Maike , Picard Daniel , Malzkorn Bastian , Custódia Carlos , Cascão Rita , Meyer Frauke-Dorothee , Blümel Lena , Göbbels Sarah , Taban Kübra , Bartl Jasmin , Bechmann Nicole , Conrad Catleen , Gravemeyer Jan , Becker Jürgen C , Stefanski Anja , Puget Stéphanie , Barata João T , Stühler Kai , Fischer Ute , Felsberg Jörg , Ayrault Olivier , Reifenberger Guido , Borkhardt Arndt , Eisenhofer Graeme , Faria Claudia C , Remke Marc 
Published: March 2022

Abstract

Background
Intratumoral heterogeneity is crucially involved in metastasis, resistance to therapy, and cancer relapse. Amplifications of the proto-oncogene MYC display notable heterogeneity at the single-cell level and are associated with a particularly dismal prognosis in high-risk medulloblastomas (MBs). The aim of this study was to establish the relevance of interclonal cross-talk between MYC-driven and non-MYC-driven MB cells.

Methods
We used fluorescence in situ hybridization, single-cell transcriptomics, and immunohistochemistry, in vitro isogenic cell models, non-targeted proteomics, mass spectrometry-based metabolite quantification, HUVECs tube formation assay, and orthotopic in vivo experiments to investigate interclonal cross-talk in MB.

Results
We found that the release of lactate dehydrogenase A (LDHA) from MYC-driven cells facilitates metastatic seeding and outgrowth, while secretion of dickkopf WNT signaling pathway inhibitor 3 from non-MYC-driven cells promotes tumor angiogenesis. This tumor-supporting interaction between both subclones was abrogated by targeting the secretome through pharmacological and genetic inhibition of LDHA, which significantly suppressed tumor cell migration.

Conclusion
Our study reveals the functional relevance of clonal diversity and highlights the therapeutic potential of targeting the secretome to interrupt interclonal communication and progression in high-risk MB.
Associations between syndesmophytes and facet joint ankylosis in radiographic axial spondyloarthritis patients on low-dose CT over 2 years
Authors: Stal Rosalinde , Sepriano Alexandre , van Gaalen Floris Alexander , Baraliakos Xenofon , van den Berg Rosaline , Reijnierse Monique , Braun Juergen , Landewé Robert B M , van der Heijde Désirée 
Published: March 2022

Abstract

Objectives
In radiographic axial spondyloarthritis (r-axSpA), spinal damage manifests as syndesmophytes and facet joint ankylosis (FJA). We evaluated whether the presence of one lesion increased the risk of the other lesion.

Methods
Patients with r-axSpA underwent low-dose CT (ldCT) and MRI of the whole spine at baseline and 2 years. On ldCT, vertebrae were scored for presence and size of syndesmophytes; facet joints were assessed for ankylosis. MR images were assessed for inflammation. Two hypotheses were tested: (i) presence of FJA is associated with new syndesmophyte(s) on the same vertebral unit (VU) 2 years later, and (ii) presence of bridging syndesmophyte(s) is associated with new FJA on the same VU 2 years later. Two generalized estimating equations models were tested per hypothesis using increase of FJA/syndesmophytes (model A) or presence of FJA/syndesmophytes (model B) as outcome, adjusted for inflammation at baseline. Secondary analyses tested the hypotheses with outcomes on adjacent VUs and dose–response effects.

Results
Fifty-one patients were included (mean age 49, 84% male, 82% HLA-B27+). Baseline bridging syndesmophytes occurred more often (range: 10–60% per VU) than FJA (range: 8–36%). Odds ratios (ORs) (95% CI) for presence of bridging syndesmophytes on development of FJA were 3.55 (2.03, 6.21) for model A and 3.30 (2.14, 5.09) for model B. ORs for presence of baseline FJA on new syndesmophytes were 1.87 (1.20, 2.92) for model A and 1.69 (0.88, 3.22) for model B. Secondary analyses yielded positive ORs for both hypotheses.

Conclusions
Bone formation in vertebrae and in facet joints influence each other’s occurrence, with the effect of syndesmophytes being larger than that of FJA.
Retrospective longitudinal assessment of ultrasound gout lesions using the OMERACT semi-quantitative scoring system
Authors: Christiansen Sara Nysom , Østergaard Mikkel , Slot Ole , Fana Viktoria , Terslev Lene 
Published: March 2022

Abstract

Objectives
The objectives of this study were (i) to evaluate the responsiveness of gout-specific US lesions representing urate deposition in patients receiving treat-to-target urate-lowering therapy using a binary and the OMERACT-defined semi-quantitative scoring systems; (ii) to determine the most responsive US measure for urate deposition and the optimal joint/tendon set for monitoring this.

Methods
US (28 joints, 14 tendons) was performed in microscopically verified gout patients initiating/increasing urate-lowering therapy and repeated after 6 and 12 months. Static images/videos of pathologies were stored and scored binarily and semi-quantitatively for tophus, double contour sign (DC) and aggregates. Lesion scores were calculated at patient level, as were combined crystal sum scores. Responsiveness of lesions—scored binarily and semi-quantitatively—was calculated at both patient and joint/tendon levels.

Results
Sixty-three patients underwent longitudinal evaluation. The static images/videos assessed retrospectively showed statistically significant decreases in tophus and DC, when scored binarily and semi-quantitatively, whereas aggregates were almost unchanged during follow-up. The responsiveness of the semi-quantitative tophus and DC sum scores were markedly higher than when using binary scoring. The most responsive measure for urate deposition was a combined semi-quantitative tophus-DC-sum score. A feasible joint/tendon set for monitoring included knee and first–second MTP joints and peroneus and distal patella tendons (all bilateral), representing the most prevalent and responsive sites.

Conclusion
The OMERACT consensus-based semi-quantitative US gout scoring system showed longitudinal validity with both tophus and DC being highly responsive to treatment when assessed in static images/videos. A responsive US measure for urate deposition and a feasible joint/tendon set for monitoring were proposed and may prove valuable in future longitudinal studies.
Multi-nutrient stoichiometry of Chinese hickory (Carya cathayensis) saplings: plant organs vary in their response to nitrogen fertilization
Authors: Xie Hongtao , Li Xueqin , Tang Yu , Pile Knapp Lauren S , Jin Songheng 
Published: March 2022

AbstractNitrogen (N) enrichment from excessive fertilization in managed forests affects biogeochemical cycles on multiple scales, but our knowledge of how N availability shifts multi-nutrient stoichiometries (including macronutrients: N, phosphorus, potassium, calcium, magnesium and micronutrients: manganese, iron and zinc) within and among organs (root, stem and leaf) remains limited. To understand the difference among organs in terms of multi-nutrient stoichiometric homeostasis responding to N fertilization, a six-level N supply experiment was conducted through a hydroponic system to examine stem growth, multi-nutrient concentrations and stoichiometric ratios in roots, stems and leaves of 2-year-old Chinese hickory (Carya cathayensis Sarg.) saplings. Results showed that N supply significantly enhanced leaf length, width, basal diameter and sapling height. Increasing the rates of N also significantly altered multi-nutrient concentrations in roots, stems and leaves. Macronutrients generally respond more positively than micronutrients within organs. Among organs, leaves and stems generally responded more actively to N supply than roots. The stoichiometric ratios of nutrients within different organs changed significantly with N supply, but their direction and degree of change varied by organ. Specifically, increased N supply reduced the ratios of both macronutrients and micronutrients to N in plant organs, while increased N supply elevated the ratios of P to other nutrients. With N fertilization, ratios of micronutrients decreased in leaves and stems and increased in roots. In particular, leaf N and stem Mn stoichiometries responded strongly to N availability, indicating stimulated N uptake but a decreased risk of Mn2+ accumulation to excessive N. Overall, Chinese hickory saplings responded positively to increasing N availability in terms of stem growth, but the multi-nutrient stoichiometric homeostasis was distinctively organ-dependent. These results are expected to enhance our understanding of N-induced changes in homeostasis of multiple nutrients at the organ level and may offer new insights into how plants adapt to increasing N fertilization.
Replacement of the Gamma by the Delta variant in Brazil: Impact of lineage displacement on the ongoing pandemic
Authors: Giovanetti Marta , Fonseca Vagner , Wilkinson Eduan , Tegally Houriiyah , San Emmanuel James , Althaus Christian L , Xavier Joilson , Nanev Slavov Svetoslav , Viala Vincent Louis , Ranieri Jerônimo Lima Alex , Ribeiro Gabriela , Souza-Neto Jayme A , Fukumasu Heidge , Lehmann Coutinho Luiz , Venancio da Cunha Rivaldo , Freitas Carla , Campelo de A e Melo Carlos F , Navegantes de Araújo Wildo , Do Carmo Said Rodrigo Fabiano , Almiron Maria , de Oliveira Tulio , Coccuzzo Sampaio Sandra , Elias Maria Carolina , Covas Dimas Tadeu , Holmes Edward C , Lourenço José , Kashima Simone , de Alcantara Luiz Carlos Junior 
Published: March 2022

Abstract
The coronavirus disease 2019 (COVID-19) epidemic in Brazil was driven mainly by the spread of Gamma (P.1), a locally emerged variant of concern (VOC) that was first detected in early January 2021. This variant was estimated to be responsible for more than 96 per cent of cases reported between January and June 2021, being associated with increased transmissibility and disease severity, a reduction in neutralization antibodies and effectiveness of treatments or vaccines, and diagnostic detection failure. Here we show that, following several importations predominantly from the USA, the Delta variant rapidly replaced Gamma after July 2021. However, in contrast to what was seen in other countries, the rapid spread of Delta did not lead to a large increase in the number of cases and deaths reported in Brazil. We suggest that this was likely due to the relatively successful early vaccination campaign coupled with natural immunity acquired following prior infection with Gamma. Our data reinforce reports of the increased transmissibility of the Delta variant and, considering the increasing concern due to the recently identified Omicron variant, argues for the necessity to strengthen genomic monitoring on a national level to quickly detect the emergence and spread of other VOCs that might threaten global health.
The Effect of Apical Vertebra Position on Growing Rod Treatment: A Clinical and Finite Element Study
Authors: Dursun Gokay , Cetik Riza M. , Guzel Dilek , Demirkiran Halil Gokhan , Ramazanov Rafik , Dede Ozgur , Gurses Ercan , Yazici Muharrem 
Published: March 2022

Background:
Growing rods (GRs) is a commonly utilized technique for the management of early-onset scoliosis. The significance of the position of the apical vertebrae relative to the rods is not known. The purpose of this study is to analyze the potential effects of the position of the apical vertebrae in relation to the GRs on deformity control through plain radiographs and finite element analysis (FEA) modeling.

Methods:
We identified 140 patients treated with GR between 2000 and 2018. Patients who had a congenital vertebral anomaly or <2-year follow-up were excluded. Curve magnitude, traction radiograph under general anesthesia (TRUGA) flexibility, apical rotation, the lengths of T1-12, T1-S1, and the instrumented segments were recorded. Patients were divided into 3 groups according to the apical position on the postoperative radiographs: group 1 (both pedicles are between the rods), group 2 (convex rod is between the apical vertebra pedicles), group 3 (both pedicles are lateral to the convex rod). FEA models were created simulating the 3 groups. Both radiographic and FEA data were analyzed to compare the deformity control and growth in each group.

Results:
Fifty-eight patients were included in the final analyses (mean age 84 mo; range: 38 to 148). Ten patients (17%) were in group 1, 34 (59%) in group 2, and 14 (24%) in group 3. Difference between TRUGA flexibilities was statistically insignificant. Group 3 was the least successful in terms of both height gain and rotational control. FEA showed a decrease in rotation and displacement for every group, however, the residual rotation and displacement was highest in group 3.

Conclusions:
Bringing the apex in line with the GR increases the capacity of growth preservation as it results in largest height gain and better deformity control. FEA model demonstrated that distraction alone is inadequate for controlling rotation, and with increasing apical translation, residual rotation after distraction also increases.

Level of Evidence:
Level III.
Lower Eyelid Reconstruction Using a Myotarsocutaneous Flap while Considering the Superior and Inferior Palpebral Sulci
Authors: Takasu Hiroyuki , Yagi Sasagu , Taguchi Sawa , Furukawa Soichi , Ono Nao , Shimomura Yutaka 
Published: March 2022

Summary:
Various reconstructive procedures have been reported for full-thickness defects of the lower eyelid after tumor excision or trauma. In eyelid reconstruction, not only functionality but also cosmetic results are important; furthermore, making scars inconspicuous is challenging. The purpose of this study is to make the scars less noticeable in lower eyelid reconstruction. We reconstructed the anterior lamella using a myotarsocutaneous flap and the posterior lamella of the donor site using a palatal mucosal graft in a 61-year-old man with basal cell carcinoma of the lower eyelid. In designing the myotarsocutaneous flap, we matched the upper edge of the flap with the superior palpebral sulcus, and the lower edge with the inferior palpebral sulcus. The flap length was the same as the width of the defect. The lateral side of the postoperative scar was hidden by the excess skin of the upper eyelid, while the caudal side of the scar and the trapdoor deformity was covered with a tear trough. After movement, the flap was not located outside the lateral canthus, and good cosmetic results were obtained. This report describes our surgical procedure.
A Rare Presentation of Hand TB Extending to the Space of Parona
Authors: Al Lahham Salim , Aljassem Ghanem , Asnaf Ayman , Omari Rand Y. , Alyazji Zaki , Sada Ruba 
Published: March 2022

Summary:
Most cases of hand infections are caused by gram-positive cocci and gram-negative bacilli. Atypical hand infections are rare and are caused by uncommon pathogens like Mycobacterium tuberculosis (TB). Musculoskeletal tuberculosis accounts for 20% of TB cases, and only 2% of these cases involve the hand and foot. In this article, we describe a case of hand TB that had spread from the thenar space to the space of Parona. The patient was treated with a combined surgical and medical approach. A 29-year-old male patient presented to our clinic with the complaint of pain and swelling of the right hand that had been worsening for 4 months before presentation. It was associated with mild weakness and night sweats. On physical examination, he had two separate swellings: one at the thenar eminence and one at the volar side of the wrist. He was taken for incision, which showed caseous material on both sites. It was sent for culture that revealed mycobacterium tuberculosis. The patient was placed on anti-TB medications for a total of 6 months. Tuberculous infection of the hand is a rare condition; the most common musculoskeletal sites are the spine, hip, and knee. Early diagnosis and treatment of TB infection in hand are essential for retaining optimal function. The diagnosis usually depends on the clinical presentation supported by laboratory tests and imaging studies. Treatment consists of medical, surgical, or combined treatment. Surgical intervention should aim to remove all the infected material in addition to debridement of the involved tissues.
Facial Feminization Surgery: A Systematic Review of Perioperative Surgical Planning and Outcomes
Authors: Tirrell Abigail R. , Abu El Hawa Areeg A. , Bekeny Jenna C. , Chang Brian L. , Del Corral Gabriel 
Published: March 2022

Background:
Facial feminization is a critical step in a transfeminine patient’s surgical transition. However, the existing literature on the various types of feminization surgeries suffers from inadequate reporting on perioperative aspects of care, such as preoperative evaluation and postoperative outcomes. The aim of this study is to evaluate facial feminization surgery (FFS), preoperative planning, and patient reported outcomes after various types of procedures.

Methods:
An electronic database search of Ovid MEDLINE was completed according to PRISMA guidelines for articles pertaining to FFS. Study characteristics, operative information, and patient demographics were collected. Data concerning preoperative imaging, virtual simulation, postoperative complications, and patient-reported outcome measures (PROMs) were collected and analyzed for patterns.

Results:
A total of 22 papers representing 1302 patients were included for analysis. The most commonly discussed operations included upper face procedures, particularly of the forehead (17 studies, 77%). When discussed, preoperative planning for FFS included standard photography in 19 (86%) studies, advanced imaging, such as cephalometry or computed tomography, in 12 (55%) studies, and virtual simulation of surgical outcomes in four (18%) studies. Patient-centered outcomes, such as postoperative satisfaction, were described in 17 (77%) studies. Standardized PROMs were heterogenous across included studies with only 11 (50%) including at least one PROM.

Conclusions:
FFS is common, safe, and highly satisfying for transfeminine patients seeking surgical intervention for identity actualization. Future research concerning transgender care must evaluate advanced surgical planning and 3D simulation combined with more standardized assessment of PROMs to ensure high-quality analysis of patient satisfaction.
Corrective Tendon Repair with Local Anesthesia following Wrist-level Replantation
Authors: Lu Yi , Hsieh Chi-Ying , Chang Che-Ning , Chang Ke-Chung , Chang Dun-Hao 
Published: March 2022

Summary:
Wide-awake local anesthesia with no tourniquet has become a popular method to ensure correct tendon condition intraoperatively. We report the case of a 17-year-old man who underwent nearly total left wrist amputation and successful replantation. The misconnection of flexor tendons between the thumb, index, and middle finger was found postoperatively. At 1.5 months postreplantation, simultaneous tenolysis and corrective tendon repair were performed under local anesthesia. The proximal tendon origins were confirmed intraoperatively with the patient awake. Six months after replantation, the patient underwent extensor pollicis longus tendon shortening and pulley reconstruction under wide-awake local anesthesia with no tourniquet for thumb extension lag. After adequate rehabilitation, the functional outcome was satisfactory. This case report demonstrates that local anesthesia is a judicious method to intraoperatively facilitate correct tendon repair, tension adjustment, and direct conversation between the surgeon and the patient.
A Description of ICNIRP’S Independent, Best Practice System of Guidance on the Protection of People and the Environment from Exposure to Non-Ionizing Radiation
Authors:   International Commission on Non-Ionizing Radiation Protection
Published: March 2022

Abstract
In this statement, the International Commission on Non-Ionizing Radiation Protection (ICNIRP) presents its structure, its activities, and general approach to providing guidance on NIR protection. The statement highlights ICNIRP’s independence and presents the principle and requirements of no commercial or other vested interests. ICNIRP’s funding arrangements and collaboration with other advisory bodies and radiation protection authorities are also described. The statement also presents the types of guidance documents that are produced by ICNIRP and the general approach in assessing scientific evidence.