bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of diabetic retinopathy in women with pregestational diabetes during pregnancy and the postpartum
Authors: Widyaputri Felicia , Rogers Sophie L. , Khong Edmund W. C. , Nankervis Alison J. , Conn Jennifer J. , Sasongko Muhammad B. , Shub Alexis , Fagan Xavier J. , Guest Daryl , Symons Robert C. A. , Lim Lyndell L. 
Published: May 2022

AbstractBackgroundDiabetic retinopathy (DR) may be affected by pregnancy. The majority of prevalence data regarding DR in pregnancy predate the advent of contemporary guidelines for diabetes management during pregnancy. This study reports DR prevalence and associated risk factors in women with pregestational diabetes during pregnancy and the postpartum in Australia.MethodsA total of 172 pregnant women with type 1 (T1DM) or type 2 diabetes diagnosed pre‐pregnancy were prospectively recruited from two obstetrics hospitals in Melbourne (November 2017–March 2020). Eye examinations were scheduled in each trimester, at 3‐, 6‐, and 12‐months postpartum. DR severity was graded from two‐field fundus photographs by an independent grader utilising the Airlie House Classification. Sight‐threatening DR (STDR) was defined as the presence of diabetic macular oedema or proliferative DR.ResultsOverall, 146 (84.9%) women had at least one eye examination during pregnancy. The mean age was 33.8 years (range 19–51), median diabetes duration was 7.0 years (IQR 3.0–17.0), 71 women (48.6%) had T1DM. DR and STDR prevalence during pregnancy per 100 eyes was 24.3 (95% CI 19.7–29.6) and 9.0 (95% CI 6.1–12.9); while prevalence in the postpartum was 22.2 (95% CI 16.5–29.3) and 10.0 (95% CI 5.4–17.9), respectively. T1DM, longer diabetes duration, higher HbA1c in early pregnancy, and pre‐existing nephropathy were significant risk factors.ConclusionsThe prevalence of DR in pregnant women was similar to the non‐pregnant diabetic population in Australia. One in nine participants had STDR during pregnancy and the postpartum, highlighting the need to optimise DR management guidelines in pregnancy given the significant risk of vision loss.
Day‐case discharge criteria and safety of children undergoing adenoidectomy and tonsillectomy for obstructive symptoms—A systematic review
Authors: Gowda Siri , Leong Wei S. , Edafe Ovie 
Published: May 2022

AbstractObjectivesObstructive sleep apnoea (OSA) is a common indication for adenoidectomy and tonsillectomy in children. Traditional practice involves overnight admission to monitor for respiratory complications. However, there is a shift towards same‐day discharge in selected patients. This systematic review aims to critically evaluate day‐case criteria and safety in children with OSA undergoing adenotonsillectomy.DesignWe performed a systematic search of EMBASE, Medline and the Cochrane library. All data collected were independently validated for accuracy. Quality assessment of included articles was performed. The protocol was registered with PROSPERO.ResultsA total of 15 studies were included (10 731 patients). There was heterogeneity in methods used to ascertain OSA, day‐case discharge criteria and lack of prospective discharge protocol. The proportion of children considered for planned day‐case surgery ranged from 28.7%–100% based on individual criteria, with an average rate of successful same‐day discharge of 96.1% in these patients. The reported rates of post‐operative respiratory adverse events and need for airway intervention were 0%–27.3% and 0.4%–6.8%, respectively. There was no reported mortality. The studies were considered low to medium on quality assessment.ConclusionThere is a lack of prospective data on day‐case criteria and systematic assessment of post‐operative complications in children with OSA undergoing adenoidectomy and tonsillectomy. However, current literature suggests that day‐case surgery is safe in carefully selected patients. Better characterisation of patient‐specific risk factors is needed to develop an optimal criteria‐based timeline for safe discharge. This has the potential to improve confidence and uptake across units.
The missing heritability in type 1 diabetes
Authors: Pang Haipeng , Lin Jian , Luo Shuoming , Huang Gan , Li Xia , Xie Zhiguo , Zhou Zhiguang 
Published: May 2022

AbstractType 1 diabetes (T1D) is a complex autoimmune disease characterized by an absolute deficiency of insulin. It affects more than 20 million people worldwide and imposes an enormous financial burden on patients. The underlying pathogenic mechanisms of T1D are still obscure, but it is widely accepted that both genetics and the environment play an important role in its onset and development. Previous studies have identified more than 60 susceptible loci associated with T1D, explaining approximately 80%‐85% of the heritability. However, most identified variants confer only small increases in risk, which restricts their potential clinical application. In addition, there is still a so‐called ‘missing heritability’ phenomenon. While the gap between known heritability and true heritability in T1D is small compared with that in other complex traits and disorders, further elucidation of T1D genetics has the potential to bring novel insights into its aetiology and provide new therapeutic targets. Many hypotheses have been proposed to explain the missing heritability, including variants remaining to be found (variants with small effect sizes, rare variants and structural variants) and interactions (gene–gene and gene–environment interactions; e.g. epigenetic effects). In the following review, we introduce the possible sources of missing heritability and discuss the existing related knowledge in the context of T1D.
Tooth resorption—Part 2: A clinical classification
Authors: Abbott Paul V. , Lin Shaul 
Published: May 2022

AbstractTooth resorption is either a physiological or a pathological process resulting in loss of dentin and/or cementum. It may also be associated with bone loss. Currently there is no universal classification for the different types of tooth resorption. This lack of a universal classification leads to both confusion amongst practitioners and poor understanding of the resorptive processes occurring in teeth which can result in incorrect/inappropriate diagnoses and mis‐management. When developing a classification of diseases and/or conditions that occur within the body, several criteria should be followed to ensure a useful classification. The classification should not only include pathological conditions but also physiological conditions. Since tooth resorption can be either pathological or physiological, a classification of tooth resorption should include both of these categories. Any classification of diseases should be possible to use clinically, meaningful, useful, clear and universal. It should enable easy storage, retrieval and analysis of health information for evidenced‐based decision‐making. It should also be possible to share and compare data and information between different institutions, settings and countries. A classification of tooth resorption should be developed by combining anatomical, physiological and pathological approaches. For some types of resorption, the aetiological approach should also be incorporated. A classification of tooth resorption that uses simple, relevant and appropriate terminology based on the nature and location of the resorptive process occurring in teeth is proposed. There are two broad categories of internal and external tooth resorption which are sub‐divided into three types of internal tooth resorption (surface, inflammatory, replacement) and eight types of external tooth resorption (surface, inflammatory, replacement, invasive, pressure, orthodontic, physiological, idiopathic). The clinician's understanding, diagnosis and management of tooth resorption can be facilitated by using this simple classification which should ideally be used universally by the entire dental profession to ensure clarity and to avoid confusion.
Predictive biomarkers of response to bacillus Calmette‐Guérin immunotherapy and bacillus Calmette‐Guérin failure for non‐muscle invasive bladder cancer
Authors: Wang Ziting , So Wei Zheng , Loh Kep Yong , Lim Yew Koon , Mahendran Ratha , Wu Qing Hui , Chiong Edmund 
Published: May 2022

AbstractWithin the heterogeneous population of patients with bacillus Calmette‐Guérin failure, there are clear differences in prognosis and therapy with regard to the timeline when bacillus Calmette‐Guérin failure occurred. There are a variety of classifications which include bacillus Calmette‐Guérin refractory disease, relapsing, unresponsive, and intolerant. Further profiling of these patients may help to shed light on other forms of therapy that are less radical. We hereby summarize the different biomarkers that predicts for response to bacillus Calmette‐Guérin immunotherapy and bacillus Calmette‐Guérin failure for non‐muscle invasive bladder cancer.
Impact of a pharmacist‐led thiopurine monitoring service in outpatients with inflammatory bowel disease
Authors: Rodda Sheridan E. , Fildes Karina J. , Shelton Edward , Goldberg Rimma , Moore Gregory T. 
Published: May 2022

AbstractBackgroundThiopurines are effective therapies for inflammatory bowel disease (IBD); however, treatment comes with safety concerns and adverse effects. Knowledge of the impact of pharmacists performing thiopurine monitoring is limited.AimsTo determine the impact of a pharmacist‐led monitoring service in patients with IBD commencing thiopurine therapy managed in the ambulatory care setting.MethodsPatients commencing thiopurine therapy for IBD pre‐ and post‐introduction of a pharmacist‐led monitoring intervention were assessed. Pre‐intervention patients received standard of care, while the post‐intervention cohort was managed by the pharmacist. Data were acquired via retrospective audit of hospital medical records. The primary end‐point was the proportion of patients with documented review for thiopurine adverse effects within the initial 3 weeks. Secondary end‐points included achievement of therapeutic drug levels, persistence with thiopurine therapy, IBD‐related episodes of care and number of outpatient medical reviews.ResultsPre‐ and post‐intervention cohorts comprised of 37 and 33 patients respectively. Pharmacist intervention increased the proportion of patients with documented monitoring within 3 weeks from 8.1% to 84.8% (P < 0.01). No difference in thiopurine dose optimisation was seen (27% vs 27.3%). Persistence with thiopurine therapy increased from 65.7% to 87.9% (P < 0.03) at 6 months. IBD‐related emergency department presentations were not significantly decreased (8.1% vs 3%; P = 0.62). No significant change was observed in hospital admissions (16.2% vs 12.1%; P = 0.74) or outpatient medical reviews.ConclusionsPharmacist monitoring of thiopurine therapy initiation in IBD outpatients improves adverse effect monitoring and increases medication persistence.
Rain events at maturity severely impact the seed quality of psyllium (Plantago ovata Forssk.)
Authors: Cowley James M. , McNeil David L. , Lui King Yin , Barsby Jacqueline P. , Ciani Silvano , Cerne Virna , Burton Rachel A. 
Published: May 2022

AbstractPlantago ovata Forssk. is an emerging crop yielding psyllium husk, a material comprised of hydrophilic polysaccharides that form mucilage upon wetting. Psyllium husk has important industrial uses including as a dietary fibre supplement and a textural alternative in gluten‐free bread production. Industrial applications require high‐quality and purity psyllium husk, but consistent supply of uniform quality material is often limited by climatic constraints, especially unseasonable rainfall at crop maturity. Here we compared the seed quality of four P. ovata varieties harvested before and after 26 mm of rain and validated our key findings in the following season. Colourimetry showed that the rain event caused the seeds to be darker and greener, possibly from pigment oxidation and microbial growth. Sugar profiling, water absorption assays and microscopy showed that premature hydration of the husk in rain‐damaged samples caused loss of the most soluble mucilage components and an increase in non‐mucilage contaminants, leading to a reduction in seed water absorption capacity, which is a key indicator of psyllium husk functionality. Germination was also diminished in rain‐affected seeds. In this study we show for the first time the extent that unseasonable rain at maturity has on P. ovata seed quality. We suggest that rain‐damaged seeds are unsuitable for husk production and resowing and outline potential screening methods to identify rain‐damaged seeds before purchase. Additionally, the extensive quality impacts described here may make P. ovata a suitable model or indicator species for studying acute climate effects on seed quality, especially from rain.
Effectiveness of compost inoculated with phosphate solubilizing bacteria
Authors: Ahmad Areesha , Zafar Urooj , Khan Adnan , Haq Tooba , Mujahid Talat , Wali Mahreen 
Published: May 2022

Abstract

Aims
The present study was carried out to design a phosphate solubilizing bacterial (PSB)-based biofertilizer using locally produced fruit waste.

Method and results
Two PSB strains Pseudomonas aeruginosa CMG4 and AAC1 were inoculated into compost. Six compost piles were prepared with carbon:nitrogen (C:N) ratio 30:1. Four piles were inoculated with PSB and two piles served as a control. After 125 days, composts were considered mature at 29–31°C in the pH range of 7.1–7.3 and 32%–35% moisture content (MC). Accessible calcium (Ca) content increased up to 50 g kg−1. Microbial analysis showed the survival of P. aeruginosa species in the maturing compost even at higher temperature (~53°C). Native bacterial load was retrieved in the range of 109–1011 CFUg−1. Heavy metal concentrations including copper (Cu), lead (Pb), chromium (Cr) and cadmium (Cd) were found to occur below critical thresholds. Seed germination index for compost toxicity was found to be &gt;80%, significantly higher than animal manure and chemical fertilizer, that is, 78% and 31%, respectively, suggesting non-toxicity.

Conclusions
The evaluation of prepared compost by physicochemical parameters revealed that inoculation of P. aeruginosa does not affect the temperature, MC, carbon to nitrogen ratio, organic matter and Mg content but significantly increased the accessible Ca content, suggesting the solubilization of inorganic Ca bound phosphate. Compost was safe in terms of heavy metal concentration and seed germination.

Significance and impact of study
This study encourages that the PSB-rich tailored compost can be utilized as a phosphatic biofertilizer to fulfil the demand for phosphorus which would improve and sustain soil fertility.
Antimicrobial potential of culturable actinobacteria isolated from the Pacific oyster Crassostrea gigas (Bivalvia, Ostreidae)
Authors: Cera Guillermo , Risdian Chandra , Pira Hani , Wink Joachim 
Published: May 2022

Abstract

Aims
Explore the diversity of culturable actinobacteria isolated from the Pacific oyster Crassostrea gigas with special emphasis on their antimicrobial activity.

Methods and Results
For the characterization of the isolated actinobacteria, a polyphasic approach was adopted and thereby phenotypic descriptions, phylogenetic analysis, evaluations of antimicrobial activities and chemical analyses of crude extracts through HPLC and LC-HRESIMS were performed. Five strains were isolated from C. gigas. The 16S rRNA gene analysis revealed that three of them were taxonomically affiliated to the genus Streptomyces and the other two strains were related to Micromonospora. High inhibition was detected against different test microorganisms such as Candida albicans, Staphylococcus aureus, Bacillus subtilis and Mycobacterium smegmatis. On the basis of the chemical analysis, 11 compounds from the active fractions of the crude extracts were determined, and 8 were related putatively to previously reported compounds.

Conclusions
Actinobacteria isolated from C. gigas represent an interesting reservoir of antimicrobial compounds, and further study to uncover the full capacity of this source is encouraged.

Significance and Impact
At present, the study of actinobacteria and their antimicrobial potential from uncommon sources as C. gigas is vital to the development of new therapeutic agents to cope with the widespread resistance of human pathogens.
Towards automatic annotation of collaborative problem‐solving skills in technology‐enhanced environments
Authors: Flor Michael , Andrews‐Todd Jessica 
Published: May 2022

AbstractBackgroundCollaborative problem solving (CPS) is important for success in the 21st century, especially for teamwork and communication in technology‐enhanced environments. Measurement of CPS skills has emerged as an essential aspect in educational assessment. Modern research in CPS relies on theory‐driven measurements that are usually carried out as manual annotations over recorded logs of collaborative activities. However, manual annotation has limited scalability and is not conductive towards CPS assessments at scale.ObjectiveWe explore possibilities for automated annotation of actions in collaborative‐teams, especially chat messages. We evaluate two approaches that employ machine learning for automated classification of CPS events.MethodData were collected from engineering, physics and electronics students' participation in a simulation‐based task on electronics concepts, in which participants communicated via text‐chat messages. All task activities were logged and time stamped. Data have been manually classified for the CPS skills, using an ontology that includes both social and cognitive dimensions. In this article, we describe computational linguistic methods for automatically classifying the CPS skills from logged data, with a view towards automating CPS assessments.ResultsWe applied two machine learning methods to our data. A Naïve Bayes classifier has been previously used in CPS research, but it is only moderately successful on our data. We also present a k‐nearest‐neighbours (kNN) classifier that uses distributional semantic models for measuring text similarity. This classifier shows strong agreement between automated and human annotations. The study also demonstrates that automatic spelling correction and slang normalization of chat texts are useful for accurate automated annotation.ImplicationsOur results suggest that a kNN classifier can be very effective for accurate annotation of CPS events. It achieves reasonably strong results even when trained on only half of the available data. This shows a promise towards reduction of manual data annotation for CPS measurement.
Stigma, epistemic injustice, and “looked after children”: The need for a new language
Authors: Fieller Danielle , Loughlin Michael 
Published: May 2022

AbstractThis article examines the processes that contribute to the stigmatization of a group of people typically identified as “children in care” or “looked after children.” In particular, we will look at the ways that we (adults, professionals, and carers) interact with these children, based on their status as both children and members of a socially marginalized and disadvantaged group, and how these modes of interaction can inhibit dialogue—a dialogue that is needed if we are to base our conceptions regarding the needs of these children on a more accurate understanding of their experiences and perspective. The problem is particularly challenging because the very terminology we use in the care community to identify this group is a product of the damaging preconceptions that have affected our interactions with its members and, we argue, it serves to reinforce those preconceptions. Using Fricker's work on epistemic injustice, in conjunction with evidence regarding how accusations of abuse and neglect of these children have been addressed in numerous cases, we illustrate the problems we have in hearing the voices of members of this group and the harmful effects this has on their own ability to understand and articulate their experiences. These problems represent “barriers to disclosure” that need to be surmounted if we are to establish a more inclusive dialogue. Currently, dialogue between these children and those of us charged to “look after” them is too often characterized by a lack of trust: not only in terms of the children feeling that their word is not taken seriously, that their claims are not likely to be believed, but also in their feeling that they cannot trust those to whom they might disclose abuse or neglect. The goals of the paper are modest in that we aim simply to open up the debate on how to meet this epistemic challenge, noting that there are specific problems that extend beyond those already identified for hearing the voices of other victims of epistemic injustice. Explicitly recognizing the nature and extent of the problem still leaves us a long way from its solution, but it is a crucial start.
Comparative pan‐genomic analysis of 51 Renibacterium salmoninarum indicates heterogeneity in the principal virulence factor, the 57 kDa protein
Authors: Saldarriaga‐Córdoba Mónica , Avendaño‐Herrera Ruben 
Published: May 2022

AbstractRenibacterium salmoninarum, a Gram‐positive intracellular pathogen, is the causative agent of bacterial kidney disease (BKD), the impacts of which are high mortalities and economic losses for the salmon industry. This study provides novel analyses for the whole‐genome sequences of 50 R. salmoninarum isolates and the reference strain ATCC 33209 using a pan‐genomic approach to elucidate phylogenomic relationships and identify unique and shared genes associated with pathogenicity and infection mechanisms. Genome size varied from 3,061,638 to 3,155,332 bp; gene count from 3452 to 3580; and predicted coding sequences from 3402 to 3527. Comparative analyses revealed an open, but approaching closed, pan‐genome. The pan‐genome analysis recovered 4064 genes, with a core genome containing 3306 genes. Phylogenetic analysis of R. salmoninarum showed high genomic homogeneity, apart from one isolate obtained from Salmo trutta in Norway. All genomes presented the 57‐kDa protein (p57). Strain ATCC 33209 and the Chilean isolates H‐2 and DJ2R presented two copies of the msa gene, while the remaining isolates had one copy. The pan‐genome analysis further identified differences in the number of copies and length of the signalling peptide for p57, the principal virulence factor reported for this bacterium. This heterogeneity could be associated with the secretion levels of p57, potentially influencing virulence. Additionally identified were numerous common genes related to iron uptake, the stress response and regulation, and cell signalling—all of which constitute the pathogenic repertoire of R. salmoninarum. This investigation provides information that is applicable in future studies for identifying therapeutic targets and/or for designing new strategies (e.g., vaccines) to prevent BKD infections in salmon farming.
Bovine meat, authoritarian populism, and state contradictions in Modi's India
Authors: Jakobsen Jostein , Nielsen Kenneth Bo 
Published: May 2022

AbstractWhile authoritarian populism and its relationship to the rural world have gained analytical prominence recently, few have attempted a systematic exploration of how various authoritarian populisms emerge from, and are embedded within, dynamics of capital accumulation, state, and class struggle. Drawing on Poulantzas' approach to “state contradictions,” we focus on the ways by which bovine meat figures in Narendra Modi's authoritarian populist project in contemporary India. On the one hand, violent authoritarianism in the country uses beef eating as a powerful tool for subjugating subaltern groups to Hindutva rule. On the other hand, the country houses a rapidly expanding beef meat agro‐industry, accounting for as much as 20% of global exports and based on corporate concentration around dominant class interests. We argue that this points to state contradictions in Modi's India witnessing strained accumulation patterns. These contradictions, we emphasize, have distinct ramifications for India's classes of labour in the countryside, as certain groups experience what we describe as a process of “double victimization.”