bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the Therapeutic Effect of ESPB for Patients Undergoing Percutaneous Vertebroplasty
Authors: Ju Peng , Jiang Dianming 
Published: February 2022

Abstract
Objective To compare the difference oftherapeutic effect between percutaneous vertebroplasty (PVP)alone and PVP + Erector spinal plane block (ESPB) in the treatment of osteoporotic vertebral compression fracture (OVCF). Methods After admission, 100 patients with osteoporotic vertebral compression fracture were randomly divided into PVP group (control group) and PVP+ESPB group (observation group) by lottery with 50 patients in each group.The visual analog pain scores (VAS) and oswestry disability index (ODI) of each group were compared before surgery, 2 hours after surgery and at the time of hospital discharge. The operation time, filling volume of intraoperative bone cement, intraoperative bloodloss, and surgery cost of each group were compared.Theearly postoperative ambulation time and early postoperative defecation (stool) time ofeach group were compared to explore the difference.Results The PVP+ESPB group had better visual analog pain scores (VAS) and oswestry disability index (ODI) at 2 hours after surgery and at the time of hospital discharge, and better early postoperative ambulation timeand early postoperative defecation (stool) timethan the PVP group (p<0.05). There were no differences in the visual analog pain scoresand oswestry disability index before surgery, operative time, filling volume of intraoperative bone cement, intraoperative blood loss, and surgery cost between the two groups, and no complications occurred between the two groups after surgery and at the time of hospital discharge. Conclusion PVP+ESPB for OVCF is associated with lower VAS, more significant pain relief, and lower oswestry disability index in patients after surgery than PVP alone for OVCF, and patients are able to engage in ambulation time earlier. Meanwhile, PVP+ESPB promotes recovery of intestinalfunction and improves patients’ quality of life so as tocontribute toquick recovery.
LncRNA RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer via upregulation of RAD51B
Authors: Wei Xinyi , Wang Conghui , Tang Sangsang , Yang Qian , Shen Zhangjin , Zhu Jiawei , Cheng Xiaodong , Wang Xinyu , Xie Xing , Xu Junfen , Lu Weiguo 
Published: February 2022

Abstract

Background Ovarian cancer (OC) has the highest fatality rate amongst all gynecologic malignancies. Exploring new factors and pathways associated with development of ovarian cancer would help to identify new therapeutic targets. Long non-coding RNAs (lncRNAs) play indispensable roles in the process of the progression of OC. Methods Reverse transcription-quantitative PCR (RT-qPCR) was performed to verify the expression of RAD51B-AS1 in cell lines and patients’ tissues. Cellular proliferation, metastasis and apoptosis were detected using Cell Counting Kit-8(CCK-8), colony formation, Transwell and flow cytometry assays, respectively. Mouse xenograft models were established to detect tumorigenesis. RT-qPCR, western blotting and immunofluorescence experiments were conducted to explore the quantitative and positional relationship between RAD51B-AS1 and its host gene, RAD51B. Co-transfection rescue experiments were then performed to preliminarily explore its mechanism in vivo and in vitro. Results RAD51B-AS1 was significantly upregulated in a highly metastatic human OC cell line and in OC tissues. RAD51B-AS1 significantly increased the proliferation and metastasis of OC cells, whilst also enhancing resistance to anoikis. Biogenetics prediction analysis revealed that the only target gene of RAD51B-AS1 was RAD51B. Subsequent gene function experiments revealed that RAD51B exerted the same biological effects as RAD51B-AS1. Rescue experiments demonstrated that the malignant biological behaviors promoted by RAD51B-AS1 overexpressing were partially or completely reversed by RAD51B silencing in vitro and in vivo.Conclusion RAD51B-AS1 promoted the malignant biological behavior of OC, and the underlying mechanism of this action may be associated with the positive regulation of RAD51B expression. The present study revealed a potentially novel molecular mechanism underlying the development of OC. RAD51B-AS1 is expected to serve as a novel molecular biomarker for the diagnosis and prediction of a poor prognosis in OC, and as a potential therapeutic target for management of the disease.
Molecular characterization of hepatitis delta virus strains isolated from patients at the Institut Pasteur de Bangui, Central African Republic, 2014 - 2017
Authors: Basimane-Bisimwa Parvine , Koyaweda Giscard Wilfrid , Demi-Sibiro Ornella Anne , Zemingui-Bambete Philippe II , Yambiyo Brice Martial , Batoumbou Marie-Yvette , Gal Frédéric Le , Gordien Emmanuel Nervile , Manirakiza Alexandre , Komas Narcisse Patrice 
Published: February 2022

Abstract
BackgroundHepatitis delta virus (HDV) can establish a persistent infection in people with chronic hepatitis B, leading to the accelerated progression of liver disease. Our study aimed to establish the prevalence of HDV and update the molecular characterization of HDV in the population carrying hepatitis B surface antigens (HBsAg) in Bangui, the capital of the Central African Republic.MethodologyWe collected 946 plasma samples from HBsAg-positive patients at the Institut Pasteur de Bangui in the Viral Hepatitis Laboratory from archived samples collected from 2014 to 2017. No information on severe liver disease or treatment history was available. Total anti-HDV antibodies (IgG) were assayed using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Subsequently, the R0 region was amplified by reverse-transcription PCR and sequenced. HDV isolates were genotyped and analyzed statistically.ResultsPrevalence of anti-HDV antibodies (IgG) was 18.18% (172/946). Of the 172 samples positive for anti-HDV antibodies, 111 HDV-positive samples (64.5%) were from males and 61 were from females (35.46%). The highest HDV prevalence (39.5%) was found in patients over 40 years old, and the lowest (1.1%) was found in the 0–10 years age group. The risk of HDV infection was very high in year 2015 (p = 0.009). Nucleotide sequences of HDV-RNA were obtained for 61 HDV-IgG positive samples of which 60 isolates belonged to the HDV-1 clade and 1 belonged to HDV-8.ConclusionA high prevalence of HDV was found in the study population, but was not evenly distributed across age groups. Patients over 40 had the highest prevalence of HDV. The study identified a predominance of the HDV-1 clade, but clade HDV-8 was also detected.
Somatic copy number alterations modulate tumor suppressor and oncogene expression to drive early onset of breast cancer
Authors: Tonouo Pamela Derliche , Bell Esther Hortense Murielle Dina , Zebaze Arnol Auvaker Tiofack , Ndounga Eliane , Ananga Sidonie Noa , Atenguena Etienne , Simo Gustave , Njouendou Abdel Jelil , Lueong Smiths Sengkwawoh 
Published: February 2022

Abstract
BackgroundFemale breast cancer (BC) is the leading cause of cancer-related deaths among women worldwide and higher mortality rates are observed in developing countries. Data from African Americans women are often used to draw inference in African BC and little is currently known about BC traits in indigenous African (IA) women.MethodsThis study prospectively enrolled 472 female patients with BC from Cameroon and Congo Brazzaville between 2007 and 2018. Patient demographics and clinical phenotypes were documented. Additional BC molecular data was downloaded from the cancer genome atlas (TCGA) and the gene expression omnibus (GEO). Demographic and clinical data from the different IA patient cohorts were analyzed to identify unique BC traits in African women. Molecular data from the TCGA was then analyzed to understand key BC traits identified in IA women.ResultsThe average age at BC diagnosis in IA women was more than 10 years earlier (46.5 ± 12.9) than within the TCGA samples (58.5 ± 13.2). Early onset of BC was observed more in IA women than in the TCGS (51.7% vs 15.6%). There was no difference in mean age at diagnosis between African American and white women. IA women had about 60% of T4 stage tumors compared with less than 5% in the TCGA cohort. Using CNA, mutation and gene expression data from the TCGA, we identified somatic copy number amplifications and upregulation of oncogenes such as CDH6, FOXM1, NAAA and CXCL10 in patients with early onset of BC. Deletion of tumor suppressor with corresponding downregulation was equally observed for TGM3, DMBT1 and MYO18B. TP53 mutations were associated with early BC onset (OR: 2.93, p < 0.01). Amplification and upregulation of the oncogene CDH6, PPP1R9B and SLC1A3 were associated with survival (HR: 0.51, 95% CI; 0.23-1.13, p = 0.096 and HR: 0.36, 95% CI: 0.18-0.68, p = 0.002, and HR: 0.14, 95% CI: 0.03-0.6, p = 0.008, respectively).ConclusionEarly onset of BC in IA women in this study might reflect yet unidentified genomic alterations. Dedicated studies are required to establish the genetic underpinning of BC in IA women to support tailored management strategies
Investigation and Analysis of Four Countries’ Recalls of Osteosynthesis Implants and Joint Replacement Implants from 2011 to 2021
Authors: Du Jun , Wang Yang , Xu Kai , Wang Yuchen , Ye Weijie , Hao Xinyi , Wang Shouli , Li Kun 
Published: February 2022

Abstract
Background Medical devices are used in almost all orthopedic surgical subspecialties, and the frequency of adverse events is increasing, which should not be ignored. To provide suggestions on how to avoid implant recalls from the perspective of manufacturers, medical institutions and supervisions, as well as how to respond promptly to adverse events.Methods The research extracted recalls of osteosynthesis implants and Joint replacement implants from January 1, 2011 to June 30, 2021 in the CNMPA, FDA, HC and ATGA websites, and collected the information on device name, recall time, recall class, recall manufacturer, device classification and affected areas. Moreover, the McKinsey 7S model and fishbone diagram were used to analyze recall reasons.Results A total of 315 cases of osteosynthesis implants and 286 cases of Joint replacement implants were reported in China, the United States, Canada and Australia. The recall number of the two implants from 2016 to 2021 were more than that from 2011 to 2015 (p = 0.012, p = 0.002) and the recall class of the two implants mainly focused on class II (76.19% and 78.32%). The affected areas were almost all over the world, mainly concentrated in developed countries. The recall reasons for both implants were similar, with process control, packaging process control, mislabeled and nonconforming materials or components for the most. But the main reasons of the two implants differed among four countries (p = 0.000).Conclusions To avoid the recalls of such implants, manufacturers should strictly select implant materials and components, develop detailed labels and instructions, severely control the packaging process, and establish the integrity of medical device data. Medical institutions should standardize procurement procedures, use qualified equipment and train medical workers. It also requires supervisions to conduct premarket safety assessments. In addition, Regulators should strengthen supervision and establish reporting systems to deal with the occurrence of adverse events promptly.
Targeting the Monocytic-Endothelial-Platelet Axis with Maraviroc and Pravastatin as a Therapeutic Option to Treat Long COVID/ Post-Acute Sequelae of COVID (PASC)
Authors: Patterson Bruce , Yogendra Ram , Jose  , Rodrigo  , Osgood Eric , Bream John , Parikh Purvi , Kreimer Mark , Kaplan Gary , Zgoda Michael 
Published: February 2022

Abstract
Post-acute sequelae of COVID (PASC), or long COVID, is a multisystem complication of SARS-CoV-2 infection that continues to debilitate millions worldwide thus highlighting the public health importance of identifying effective therapeutics to alleviate this illness. The pathophysiology behind PASC may be attributed to the recent discovery of persistent S1 protein subunit of SARS-CoV-2 in CD16+ monocytes up to 15 months after infection. CD16+ monocytes, which express both CCR5 and fractalkine receptors (CX3CR1), play a role in vascular homeostasis and endothelial immune surveillance. We believe targeting these receptors using the CCR5 antagonist, maraviroc, along with pravastatin, could disrupt the monocytic-endothelial-platelet axis that may be central to the etiology of PASC. Using five validated clinical scales (NYHA, MRC Dyspnea, COMPASS-31, modified Rankin, and Fatigue Severity Score) to measure 18 participants’ response to treatment, we observed significant clinical improvement in six to twelve weeks on a combination of maraviroc 300mg PO BID and pravastatin 10 mg PO daily. Subjective neurological (p=0.002), autonomic (p<0.0001), respiratory (p=0.0153), cardiac (p=0.002) and fatigue (p<0.0001) symptoms scores all decreased which correlated with statistically significant decreases in vascular markers sCD40L and VEGF. These findings suggest that by interrupting the monocytic-endothelial-platelet axis, maraviroc and pravastatin may restore the immune dysregulation observed in PASC and could be potential therapeutic options. This sets the framework for a future double-blinded, placebo-controlled randomized trial to further investigate the drug efficacy of maraviroc and pravastatin in treating PASC.
HydroZitLa inhibits calcium oxalate stone formation in nephrolithic rats and promotes longevity in nematode Caenorhabditis elegans
Authors: Lordumrongkiat Nalinthip , Chotechuang Nattida , Prasanth Mani Iyer , Jindatip Depicha , Ma-on Chakriwong , Chuenwisad Kamonchanok , Leelahavanichkul Asada , Tencomnao Tewin , Boonla Chanchai 
Published: February 2022

Abstract
Low fluid intake, low urinary citrate excretion, and high oxidative stress are main causative factors of calcium oxalate (CaOx) nephrolithiasis. HydroZitLa contains citrate and natural antioxidants and is developed to correct these three factors simultaneously. Antioxidants theoretically can prolong the lifespan of organisms. In this study, we preclinically investigated the antilithogenic, lifespan-extending and anti-aging effects of HydroZitLa in HK-2 cells, male Wistar rats, and Caenorhabditis elegans. HydroZitLa significantly inhibited CaOx crystal aggregation in vitro and reduced oxidative stress in HK-2 cells challenged with lithogenic factors. For experimental nephrolithiasis, rats were divided into four groups: ethylene glycol (EG), EG+HydroZitLa, EG+Uralyt-U, and untreated control. CaOx deposits in kidneys of EG+HydroZitLa and EG+Uralyt-U rats were significantly lower than those of EG rats. Intrarenal expression of 4-hydroxynonenal in EG+HydroZitLa rats was significantly lower than that of EG rats. The urinary oxalate levels of EG+HydroZitLa and EG+Uralyt-U rats were significantly lower than those of EG rats. The urinary citrate levels of EG+HydroZitLa and EG+Uralyt-U rats were restored to the level in normal control rats. In C. elegans, HydroZitLa supplementation significantly extended the median lifespan of nematodes up to 34% without altering feeding ability. Lipofuscin accumulation in HydroZitLa-supplemented nematodes was significantly lower than that of non-supplemented control. Additionally, HydroZitLa inhibited telomere shortening, p16 upregulation, and premature senescence in HK-2 cells exposed to lithogenic stressors. Conclusions, HydroZitLa inhibited oxidative stress and CaOx formation both in vitro and in vivo. HydroZitLa extended the lifespan and delayed the onset of aging in C. elegans and human kidney cells. This preclinical evidence suggests that HydroZitLa is beneficial for inhibiting CaOx stone formation, promoting longevity, and slowing down aging.
Stability of Fe5O6 and its relation to other Fe-Mg-oxides at high pressures and temperatures
Authors: Woodland Alan B. , Uenver-Thiele Laura , Ballaran Tiziana Boffa , Miyajima Nobuyoshi , Rosbach Kevin , Ishii Takayuki 
Published: February 2022

Abstract
The stability of Fe5O6 has been experimentally determined under pressure-temperature conditions relevant for the Earth’s deeper upper mantle down to the upper portion of the lower mantle (to 28 GPa). In addition, we investigated the incorporation of Mg into Fe5O6 and its systematics, which allows us to discuss the relevance of this phase for the mantle. Experiments were performed from 8–28 GPa and 900–1600 °C. Additional oxide phases may appear if the bulk composition does not maintain the Fe32+Fe23+O6 stoichiometry during the experiment, including coexisting Fe4O5 or Fe9O11. Unfortunately, the similarities in Raman spectra between several high-pressure Fe-oxide phases make this method unsuitable for distinguishing which phase is present in a given sample. The stability field for Fe5O6 extends from ~9 to at least 28 GPa but is truncated at lower temperatures by the assemblage Fe4O5 + wüstite. Refined thermodynamic properties for Fe5O6 are presented. The range of redox stability of Fe5O6 appears to be more limited than that of Fe4O5.
Solid solution along the Fe5O6-Mg3Fe2O6 binary is quite limited, reaching a maximum Mg content of ~0.82 cations per formula unit (i.e., XMg3Fe2O6 ≈ 0.27) at 1400 °C and 10 GPa. The observed sharp decrease in molar volume of the O6-phase with Mg content could be a possible explanation for the limited range of solid solution. A phase diagram has been constructed for a composition of approximately Mg0.5Fe2.52+Fe23+O6 stoichiometry. This small amount of Mg causes a significant change in the relations between the O6-structured phase and the assemblage O5-structured phase + (Mg,Fe)O. Several experiments were performed to test whether the O6-phase can coexist with mantle silicates like wadsleyite and ringwoodite. In all cases, the run products contained (Mg,Fe)2Fe2O5 rather than the O6-phase, further underlining the limited ability of Fe5O6 to accommodate enough Mg to be stable in a mantle assemblage.
The large stability field of Fe5O6 implies that this phase could likely occur in locally Fe-rich environments, like those sampled by some “deep” diamonds. However, the limited solubility of Mg in the O6-phase leads us to conclude that the O5-phase should be of much more relevance as an accessory phase in a peridotitic mantle assemblage.
Jingwenite-(Y) from the Yushui Cu deposit, South China: The first occurrence of a V-HREE-bearing silicate mineral
Authors: Liu Peng , Gu Xiangping , Zhang Wenlan , Hu Huan , Chen Xiaodan , Wang Xiaolin , Song Wenlei , Yu Miao , Cook Nigel J. 
Published: February 2022

Abstract
Jingwenite-(Y), Y2Al2V24+(SiO4)O4(OH)4, the first V-HREE-bearing silicate mineral discovered in nature, is an abundant component of a sediment-hosted stratiform Cu (SSC) deposit, Yushui, South China. The mineral occurs in bedded/massive sulfide-bearing ore and is associated with bornite, chalcopyrite, galena, xenotime-(Y), nolanite, thortveitite, roscoelite, barite, and quartz. Optically, jingwenite-(Y) is biaxial (+), with α = 1.92(4), β = 1.95(2), γ = 1.99(3) (white light), and 2V (calculated) = 83°. The dispersion is medium with r &lt; v, and the pleochroism is with X = light brown, Y = brown, Z = dark brown. The color, streak, luster, and hardness (Mohs) are light brown, yellowish gray, vitreous, and 4½–5, respectively.
Jingwenite-(Y) is monoclinic, with space group I2/a, Z = 4, and unit-cell parameters a = 9.4821(2) Å, b = 5.8781(1) Å, c = 19.3987(4) Å, β = 90.165(2)°, and V = 1081.21(4) Å3. The structure of jingwenite-(Y) has chains of edge-sharing Al(V,Fe)-O octahedra and V(Ti)-O octahedra extending along the b-axis and linked by insular Si-O tetrahedra, leaving open channels occupied by HREEs. Jingwenite-(Y) is a new nesosilicate structural type.
Sm-Nd dating and Nd isotope signatures of jingwenite-(Y) reveal an epigenetic origin and suggest that HREEs and V were added to the SSC system via leaching of abundant heavy minerals in the footwall red sandstone by oxidized basinal brines. The abundance of jingwenite-(Y) at Yushui indicates that it could potentially be a valuable resource for HREE and V. Moreover, HREE and V mineralization can also occur in the same sediment-hosted Cu mineral system.
Perancangan Iklan Layanan Masyarakat “Mencuci Tangan” Sebagai Upaya Tanggap Covid-19
Authors: Nisa Laili Nursya'baana  , Nova Kristiana  , Asidigisianti Surya Patria  
Published: February 2022

Di tempat-tempat usaha, seperti toko kelontong, toko beras, ataupun warung makan yang dimiliki oleh masyarakat umum harus menyediakan tempat mencuci tangan sebagai salah satu protokol WHO untuk pencegahan COVID-19. Tempat-tempat mencuci tangan bermunculan di depan toko atau tempat-tempat umum bersifat sementara. Biasanya terbuat dari ember atau tempat air yang diberi keran. Di tempat-tempat air tersebut tidak diberi arahan bagaimana mencuci tangan yang benar. Masyarakat sering mencuci tangan tidak sesuai dengan tata cara cuci tangan yang benar. Tujuan perancangan ini adalah merancang stiker Iklan Layanan Masyarakat (ILM) tentang Mencuci Tangan. Proses perancangan suatu karya desain grafis yaitu stiker memerlukan data yang akurat untuk dianalisis dalam mendukung konsep yang melandasi pembuatan karya. Sumber data primer atau utama diperoleh dari pengamatan/observasi cara mencuci tangan yang betul, baik dari video, pengamatan langsung dan dari selebaran (flyer) yang dikeluarkan oleh Kementerian Kesehatan. Sumber data sekunder adalah data yang tidak langsung memberikan data pengumpul data melalui. Data ini didapat dari studi literatur, baik diperoleh dalam buku maupun artikel dan foto-foto yang diperoleh berdasarkan hubungan dengan objek perancangan. Informasi diperoleh dari beberapa buku atau artikel yang mencangkup informasi mengenai cara mencuci tangan yang benar. Agar ILM dapat tepat sasaran dan berhasil mencapai tujuan maka perlu mempertimbangkan aspek audien. Tahap perancangan dimulai dengan menggambarkan sebuah konsep yang diperoleh dari berbagai informasi berkaitan dengan urutan mencuci tangan yang benar dalam sebuah thumbnail dan thigttisue. Hasil perancangan ini adalah stiker ILM Mencuci Tangan yang digunakan untuk kampanye sosial bagaimana mencuci tangan yang benar. Stiker ditempel di ember berkeran untuk mencuci tangan.
Perancangan Keyboard Mekanik Sinar Ultraviolet-c untuk Mencegah penyebaran Bakteri dan Virus Di Warung Internet
Authors: Muhammad Ayub Teguh Prakoso  , Dr. Rahmawan Dwi Prasetya  , Sekartaji Suminto  
Published: February 2022

Keyboard adalah perangkat yang keberadaannya sangat penting pada era modern sebagai alat yang berfungsi untuk memasukkan data berupa hussus lain huruf, angka dan perintahnya yang nantinya akan diproses oleh komputer. Menurut WHO tangan merupakan jalur utama penularan kuman dan keyboard merupakan perangkat komputer yang berkontak langsung dengan tangan manusia. Kebersihan permukaan keyboard belum tertentu terjaga dengan baik terlebih pada Fasilitas keyboard yang berada pada tempat umum seperti warnet(warung internet) yang di mana digunakan lebih dari satu pengguna. Bakteri dan virus yang menempel di permukaan keyboard dari pengguna sebelumnya sangat berbahaya bagi pengguna berikutnya, bakteri dan virus yang terdapat pada permukaan keyboard dapat memicu penyebaran penyakit melalui permukaan keyboard yang tidak bersih. Atas dasar adanya penularan bakteri dan virus serta bakteri dan virus yang dapat berkembang biak pada permukaan keyboard pada perancangan keyboard mekanik dengan sinar ultraviolet-c dapat mencegah penyebaran dan perkembangbiakan bakteri dan virus yang ada pada permukaan keyboard.