bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of the left ventricular ejection fraction by machine learning algorithms based on heart rate variability parameters in patients with ischemic heart disease
Authors: Tian Xiaochen , Lu Ping , Tao Han , Li Jing , Cai Qian , Liu Guozhen , Kang Lianghuan , Yang Min , Liu Yanjun , Lu Qinghua 
Published: August 2022

Abstract
Background
The left ventricular ejection fraction (LVEF) plays a pivotal role in the diagnosis and prediction of ischemic heart disease (IHD). Current techniques to measure LVEF have led to some complications and are relatively expensive despite the high accuracy. Heart rate variability (HRV) is an alternative for the assessment of cardiac function and its related parameters are easily to be derived from electrocardiography.
Objective
This study aimed to investigate the corresponding relationship between LVEF and HRV in patients with IHD via prediction models developed by machine learning techniques and compare the accuracy of these models.
Methods
In this retrospective real-world study, patients with IHD admitted to Jinan 4th People’s hospital between January 2019 and December 2020 were randomly selected and divided into a derivation cohort and a validation cohort in a 7:3 ratio. LVEF were measured by color Doppler echocardiography while HRV-related parameters were derived from a 24h Holter electrocardiography recordings. Patient clinical features and HRV parameters were identified and used in the subsequent modelling process. Logistic regression, naïve Bayes, decision tree, gradient boosting, extreme gradient boosting (XGBoosting) and random forests were respectively applied to estimate the correlation between HRV and LVEF. Model performances were evaluated by the coefficient R2 (the closer the result is to 1, the better) and the accuracy (a precise prediction was defined as the accuracy ≥ 90%).
Results
A total of 179 patients were included in this study with 125 (mean age 67.03 ± 16.12, male 72 [53.7%]) in the derivation cohort and 54 (mean age 62.51 ± 12.97, male 19 [48.7%]) in the validation cohort. Six variables including sex, age, SDNN, SDANN, rMSSD and pNN50 were found to be correlated with LVEF. Gradient boosting and random forests outperformed other machine learning models, showing the best prediction performance (> 90%) in the prediction of both LVEF percentage (%) and LVEF stratifications (< 40%, 40–50%, > 50%). These six indicators showed different contributions in different algorithms with sex as the least important indicator.
Conclusion
Gradient boosting and random forests machine learning models based on HRV- and patient characteristics-related parameters provided a new method in predicting LVEF in patients with IHD, which showed a potential in the diagnosis of IHD.
First case of VIM-1-producing Leclercia adecarboxylata in Oman: A case report and literature review
Authors: Shuhoumi Mohammed Al , Mhrooqi Abdulrahman Al , Rashdi Azza Al , Jardani Amina Al 
Published: August 2022

Abstract
Leclercia adecarboxylata is a recently acknowledged emerging pathogen. It is a member of the Enterobacterals family, formerly thought to be a member of the genus Escherichia. Isolation was reported from various animal and environmental specimens. However, it rarely causes infection in humans, and the true frequency is unknown or underestimated. Leclercia adecarboxylata showed an ascending resistance grade from pan-sensitive to Carbapenem-resistant due to its ability to produce and harbour hydrolyzing enzymes that challenge daily clinical practices. In our report, the isolate was misidentified as Citrobacter koseri by API E and Pantoea species by Vitek 2 but confirmed by MALDI-TOF MS and 16S ribosomal RNA analysis as Leclercia adecarboxylata. Conventional PCR revealed the presence of two populations of resistance genes, VIM-1 and OXA-48. Herein, a report of the first emergence of Leclercia adecarboxylata producing VIM-1 in a clinical sample of a 63-year-old non-immunocompromised female with acute intracerebral haemorrhage.
Gender Related Trends in Ophthalmology: Professional Career
and Personal Life Performance
Authors: Barequet Irina , Rosenblatt Amir , Fogler Michal , Pedut-Kloizman Tamar , Gaton Dan , Loewenstein Anat , Habot-Wilner Zohar 
Published: August 2022

Abstract
Background Gender-diversity trends in ophthalmology in Israel have not been studied despite a high proportion of female ophthalmologists. Our purpose was to evaluate gender-related trends of ophthalmology professional career and personal life performance in early and advanced careers in Israel.Methods A survey based on a nation-wide voluntary anonymous web questionnaire was distributed to the members of the Israeli Ophthalmological Society. The questionnaire included questions referring to professional career and personal life performance. Gender differences were analyzed. Results Out of 252 respondents, 116 (46%) were women, with a mean age of 47.97+-11.3 and 53.18+-12.4 (P=0.01) years, respectively. Marital status, post-residency fellowship performance or duration, an additional academic degree, academic appointments, and managerial positions were similar between genders. Fellowship predominance of males was found in cornea/cataract/refractive and of females in strabismus, pediatric ophthalmology, and neuro-ophthalmology (P<0.01, P=0.032, respectively). Men had significantly more publications and appointments in peer review journals and work hours (P=0.04 and P=0.02, P =0.027, respectively). Both genders responded similarly regarding work satisfaction, combination between family life and work. More women felt inequity during and post-residency in clinical (P =0.011, P=0.001, respectively) and surgical work (P=0.001, P=0.035, respectively). More women experienced inappropriate remarks from patients and sexual harassment (P=0.001, P<0.001, respectively), and supported the need for affirmative action (P<0.0001). Conclusions Several gender disparities were found among ophthalmologists in Israel, including subspecialties distributions, publications, appointments in peer review journals, working hour, work inequity sense, harassment events and support the need for affirmative action.
The Mental health related barriers and benefits to EXercise (MEX) scale
Authors: Connolly Madeleine , Bowden Stephen C. , Pascoe Michaela C. , Dam Nicholas T. Van 
Published: August 2022

Abstract
Background: Physical exercise has been shown to reduce anxiety and depression symptoms, the most common mental health disorders globally. Despite the benefits of exercise in anxiety and depression, the symptoms of these disorders may directly contribute to a lack of engagement with exercise. However, mental health-related barriers and benefits to exercise engagement have not been addressed in quantitative research. We introduce the development and psychometric validation of the Mental health-related barriers and benefits to EXercise (MEX) scale. Results: Two datasets were collected and split into three samples (sample 1 n = 492; sample 2 n = 302; sample 3 n = 303) for scale refinement and validation with exploratory and confirmatory factor analysis. We identified a 30-item, two-factor model comprising 15 barrier and 15 benefit items. Overall model fit was excellent for an item-level scale across the three samples (CFI = 0.935 to 0.951; RMSEA = 0.037 to 0.039). Internal consistency was also excellent across the three samples (α = 0.900 to 0.951). The barriers subscale was positively correlated with symptoms of anxiety, depression and stress, and negatively correlated with measures of physical activity and exercise engagement. The benefits subscale was negatively correlated with symptoms of anxiety, depression and stress, and positively correlated with measures of physical activity and exercise engagement. Conclusion: The MEX is a novel, psychometrically robust scale, which is appropriate for research and for clinical use to ascertain individual and/or group level mental health-related barriers and benefits to exercise.
Biocidal properties of Chitosan-encapsulated ternary titanium dioxide-nickel oxide-copper oxide hybrid nanomaterials were prepared via a facile one-pot precipitation process
Authors: Vishnuvardhanaraj Govindaraj , Bharathidasan Ganesan , Tamilvedan Dhanapal , Karthikeyan Chandrasekaran 
Published: August 2022

Abstract

Chitosan-encapsulated ternary titanium dioxide-nickel oxide-copper oxide (CTNC) hybrid nanomaterials (HNM) were synthesis via a facile one-pot precipitation method. The synthesized Chitosan-titanium dioxide-nickel oxide-copper oxide was characterized by XRD, UV, FTIR, DLS, FESEM, EDAX, and PL tested against G- (gram-negative) bacterial strain such asK. pneumonia,S. dysenteriae,E. coli,P. Vulgaris,P. aeruginosa, andV. cholerae, employed by the well method. The CTNC hybrid nanomaterials exhibit a more substantial antibacterial effect against gram-negative bacteria. The MDA-MB-231 cell-line, with an IC50concentration value of 9.8 g/mL was chosen to test CTNC hybrid nanomaterials' anticancer properties against human breast cancer cell lines. The toxicity studies of fibroblast L929 cells showed that the CTNC hybrid nanomaterials were less harmful to the healthy cells. As a result, the CTNC hybrid nanomaterials can be used for biomedical and industrial applications to improve human health conditions.
circPLIN2 promotes clear cell renal cell carcinoma progression by binding IGF2BP proteins and miR-199a-3p
Authors: Zhao Bin , Huang Cong , Pan Jie , Hu Hao , Liu Xiaojuan , Zhang Kaoyuan , Zhou Fenli , Shi Xin , Wu Jun , Yu Bo , Chen Xiaofan , Zhang Wei 
Published: August 2022

Abstract
Recent evidence has indicated that circular RNAs (circRNAs), as a novel type of regulatory RNA, play important roles in the development and progression of various cancers. However, the potential regulatory roles and molecular mechanisms of circRNAs in clear cell renal cell carcinoma (ccRCC) remain largely unclear. Herein, we explored the expression profiles of circRNAs in 10 paired samples of RCC (including cancer tissues and surrounding tissues) from the Gene Expression Omnibus (GEO) datasets GSE124453 and GSE108735. We initially identified hsa_circ_0086457, designated circPLIN2, which was derived from exons 4 to 5 of the PLIN2 gene. circPLIN2 was significantly upregulated in ccRCC cells and tissues, and its overexpression was correlated with higher clinical stage and worse prognosis in ccRCC patients. We observed that circPLIN2 was preferentially located in the cytoplasm and had more stability than its linear counterpart PLIN2. Moreover, gain- and loss-of-function assays demonstrated that elevated circPLIN2 promoted ccRCC cell proliferation, migration and invasion in vitro and ccRCC tumor growth in vivo. Mechanistically, circPLIN2 not only enhanced the mRNA stability of c-Myc and MARCKSL1 by binding to the KH domains of IGF2BP proteins but also competitively sponged miR-199a-3p to abolish the repressive effect of miR-199a-3p on ZEB1, which ultimately resulted in tumorigenesis and progression of ccRCC. Collectively, our results suggest that circPLIN2 may serve as a promising diagnostic and prognostic biomarker as well as a potential therapeutic target for ccRCC patients.
Neoadjuvant Checkpoint Blockade in Combination with Chemotherapy in Patients with Tripe-Negative Breast Cancer: Exploratory Analysis of Real-World, Multicenter Data
Authors: Deng Heran , Wang Liying , Wang Na , Zhang Kejin , Zhao Yanxia , Qiu Pengfei , Qi Xiaowei , Zhang Danhua , Xu Fei , Liu Jieqiong 
Published: August 2022

Abstract
Purpose
Histological subtypes of triple-negative breast cancer (TNBC) patients have an unfavorable outcome. Many randomized controlled trials have showed that neoadjuvant immunotherapy combined with chemotherapy can enhance the pathologic complete response (pCR) rate and/or long-term survival of TNBC. However, no real-world studies focusing on neoadjuvant checkpoint blockade in TNBC have been reported thus far.
Methods
A total of 63 early TNBC patients who were treated with pembrolizumab or camrelizumab in combination with chemotherapy between November 2019 and December 2021 at 7 institutions were included. The primary endpoint was pCR1 defined as ypT0/Tis and ypN0. Secondary endpoints included pCR2 defined as ypT0/Tis, overall response rate (ORR), disease-free survival (DFS), drug-related adverse events (AEs) and biomarkers.
Results
The percent of patients with pCR1 in this current study was 36.7%, and 47.6% of patients achieved a pCR2. The ORR was 82.5%. The median DFS of 33 patients with non-pCR was 20.7 months (95% CI 16.3 months-not reached). There was a significant difference in DFS between the patients who achieved a pCR and those with non-pCR tumors after neoadjuvant therapy (HR = 0.28, 95% CI 0.10–0.79; P = 0.038). The most common AEs were nausea (63.4%), fatigue (42.7%), leucopenia (30.0%) and elevated transaminase (11.7%).
Conclusion
Neoadjuvant checkpoint blockade in combination with chemotherapy may achieve a meaningful pCR and DFS, especially in high-risk TNBC patients. However, the pCR in this real-world research was slightly lower than those in clinical trials.
Epidemiology of Newcastle Disease in Village Chicken in Melokoza District, Gofa Zone, Southwest Ethiopia
Authors: Betela Yohannis , Hailegebreal Gizachew , Shiferaw Dessie 
Published: August 2022

Abstract

Poultry production contributes significantly to the livelihoods of Ethiopian farmers and the national economy, although it is hampered by different factors, including infectious diseases. A cross-sectional study was conducted from December 2019 to May 2020 in Melokoza District, Gofa Zone, Ethiopia. The objectives of the study were to estimate the seroprevalence of Newcastle disease in unvaccinated chickens and to identify the risk factors associated with the disease. A systematic random sampling technique was employed to select chickens from individual chicken owners. A total of 405 blood samples were collected and submitted to the national veterinary institute in Bishoftu, Ethiopia. Hemaglutination inhibition test was performed to detect antibodies from the collected chickens’ serum. Both univariable and multivariable logistic regression analyses were performed by using the STATA statistical software package. The test result showed that the overall seroprevalence was 68.8% (95% CI: 64%, 73%). The highest seroprevalence of 86% (58/67) was recorded in Gazar kebele (lowland), whereas the lowest seroprevalence of 45% (32/71) was recorded in Maizelo (highland). Sex, age, altitude and management practice risk factors showed significant associations (p ˂ 0.05) with the disease prevalence. In conclusion, this study emphasized the prevailing higher prevalence of Newcastle disease in free-scavenging chickens. Regular vaccination for Newcastle disease is therefore recommended. Further studies are warranted to better understand the circulating strain and its economic effect on backyard poultry production in the study area.
Validation of reference genes for the normalization of the RT- qPCR in peripheral blood mononuclear cells of septic patients
Authors: Song Ruoyu , He Shijun , Wu Yongbing , Chen Wanxin , Zhu Yaxi , Chen Huan , Wang Qianlu , Wang Shouman , Xiao Lan , Tan Sichuang , Tan Sipin 
Published: August 2022

Abstract
Objective To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC). Methods Total RNA in PBMCs was extracted and RT-qPCR was used to determine the mRNA expression profiles of 9 candidate genes, including ACTB, B2M, GAPDH, GUSB, HPRT1, PGK1, RPL13A, SDHA and YWHAZ. The genes expression stabilities were assessed by both geNorm and NormFinder software. Results YWHAZ was the most stable gene among the 9 candidate genes evaluated by both geNorm and NormFinder in mixed and sepsis groups. The most stable gene combination in mixed group analyzed by geNorm was the combination of GAPDH, PKG1 and YWHAZ, while that in sepsis group was the combination of ACTB, PKG1 and YWHAZ. Conclusion Some commonly used stably expressed reference genes might be up-regulated or down-regulated in septic patients compared with healthy people. Of all nine genes studied, YWHAZ was found to be the best reference gene for studying the PBMC gene expression in septic patients. The best reference gene combination for septic patients’ investigation was ACTB, PKG1 and YWHAZ. We provide an important data for selecting the most stable reference genes to standardize RT-qPCR data, gene expression verification of sepsis and future gene function exploration.
Awareness and needs of smoking cessation services for female emotional laborers, parcel delivery workers, transportation workers, and construction workers in Korea
Authors: Lee Dahyeon , Lee Kang-Sook , Kim Haena , Lee Yeonwoo , Lee Mi‑Ji , Lee Hyun Kyung , Myong Jun-Pyo , Kim Hyekyeong , Lee Jakyoung 
Published: August 2022

Abstract
Background
With the coronavirus disease 2019 (COVID-19) and consequent COVID-19 blues striking the world, cigarette sales rose by 5.4% in 2020 compared to the previous year. Female emotional labor, parcel delivery, transportation, and construction workers are vulnerable classes for smoking compared to office workers. Although South Korea has implemented various smoking cessation clinics, quitlines, and smoking cessation services, these occupations have poor access to these services. This study evaluated smoking related characteristics and awareness, as well as needs for smoking cessation services, among female emotional laborers, parcel delivery workers, transportation workers, and construction workers.
Methods
A total of 819 female emotional laborers, parcel delivery workers, transportation workers, and construction workers aged 19 years and above were recruited nationwide. After excluding participants with missing data, 808 were included in the final analysis. Participants’ age, marital status, weekly work hours, job stress, age at cigarette smoking initiation, average daily smoking, types of tobacco products, smokers among close people, attempts to quit smoking, plans to quit smoking, awareness of smoking cessation services, and needs for smoking cessation services were surveyed.
Results
Compared with parcel delivery workers, attempts to quit smoking were significantly higher among female emotional laborers (OR = 11.18, CI = 6.32–19.79), transportation workers (OR = 2.52, CI = 1.42–4.47), and construction workers (OR = 1.78, CI = 1.17–2.73), and plans to quit smoking were also significantly higher among female emotional laborers (OR = 5.43, CI = 3.57–8.26), transportation workers (OR = 3.42, CI = 2.14–5.46), and construction workers (OR = 2.27, CI = 1.52–3.40). Prior utilization of smoking cessation services was also higher among female emotional laborers (OR = 8.81, CI = 5.60–13.86), transportation workers (OR = 3.92, CI = 2.40–6.40), and construction workers (OR = 1.40, CI = 0.95–2.05). Awareness of smoking cessation services was significantly lower among construction workers (OR = 0.40, CI = 0.18–0.91) compared to parcel delivery workers.
Conclusions
Visiting smoking cessation programs and mobile applications to help quit smoking are needed for parcel delivery workers, and their work environment should also be improved. Smoking cessation services should be promoted at work sites, and smoking related education should be provided and promoted among managers.
Splenic artery ligation ameliorates postoperation liver function in HCC who underwent hepatectomy
Authors: Wang Dong , Long Guo , Fang TongDi , Zhou LeDu 
Published: August 2022

Abstract
Background: Posthepatectomy liver failure (PHLF) is one of the leading causes of death in hepatocellular carcinoma (HCCs). Our reaserch aim to analyze the hepatectomy combined with splenic artery ligation on postoperation liver function recovery in HCCs. We hypothesized that the hepatectomy combined with splenic artery ligation can accelerated postoperative recovery without increasing the risk of surgery.Methods：75 HCC patients were included in our study, 62 patients underwent liver resection (LR), 13 patients underwent hepatectomy combined with splenic artery ligation group (HSAL). We compared the liver function and complications in LR and HSAL group post operatation. Results：There were no statistically significant differences in sex and other parameters pre-operative between LR and HSAL group. There were statistically significant differences between two groups in the ALT, bilirubin, PT on the 3-day, and the bilirubin, PT on the 5-day postoperation (P<0.05). The complications and mortality between the two groups had no differences. Our findings indicated that HSAL promotes liver function recovery and is safe and reliable for HCCs. Interestingly, we found that patients are more likely to occurs PHLF who had spleen volume over 639 cm3, and spleen volume is a factor to select patients who are suitable for HSAL. Conclusions：We conclude that HSLA can significantly promote postoperative liver function recovery and reduce the risk of PHLF who had excessive spleen volume.
Adipose transplantation improves olfactory function and neurogenesis via PKCα-involved lipid metabolism in Seipin Knockout mice
Authors: Yang Jing , Yang Na , Zhao Huifang , Qiao Yan , Li Yanqiu , Wang Chunfang , Lim Kah-Leong , Zhang Chengwu , Yang Wulin , Lu Li 
Published: August 2022

Abstract
Lipodystrophy-associated Metabolic Disorders caused by Seipin deficiency lead to not only severe lipodystrophy but also neurological disorders. However, the underlying mechanism of Seipin deficiency-induced neuropathy is not well elucidated and the possible restorative strategy needs to be explored. In the present study, we investigated the systemic lipid metabolic abnormalities of Seipin knockout (KO) mice and their effect on adult neurogenesis in the subventricular zone (SVZ) and olfactory function. It was found that KO mice presented an ectopic accumulation of lipid in the lateral ventricle, accompanied by decreased neurogenesis in adult SVZ, diminished new neuron formation in the olfactory bulb, and impaired olfactory-related memory. Transcriptome analysis showed that the differentially expressed genes (DEGs) in SVZ tissues of adult KO mice were significantly enriched in biological processes related to lipid metabolism. Mass spectrometry imaging showed that the levels of glycerophospholipid, diglyceride and ceramide were significantly increased. In the restorative study, we found that subcutaneous adipose tissue transplantation (AT) rescued the abnormality of peripheral metabolism in KO mice and ameliorated the ectopic lipid accumulation in SVZ, concomitant with restoration of the SVZ neurogenesis and olfactory function. Mechanistically, PKCα was the potential mediator of lipid dysregulation-induced phenotypes. In the brain tissue of KO mice, PKCα was upregulated, which could be mimicked by the administration of DG analogue (Dic8) into cultured neural stem cells (NSCs). Dic8 impaired proliferation and differentiation NSCs, whereas it could be recovered by PKCα inhibitor. Overall, this study demonstrates that Seipin deficiency leads to systemic lipid metabolism disorder, which impairs neurogenesis and olfactory memory. Adipose transplantation restores lipid metabolic homeostasis and neurogenesis via PKCα involved pathway. The present study paves a novel way to treat lipid metabolic dysregulation-induced neurological disorders.