bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Polychlorinated biphenyls (PCBs) in Iranian dairy products (yogurt, doogh and kashk) by modified QuEChERS extraction and GC- QqQ-MS/MS method: a health risk assessment study
Authors:   Amin Kiani1 Amin Kiani1, Arabameri Majid , Moazzen Mojtaba , Shariatifar Nabi , Tooryan Fahimeh , Ghanbariasad Ali , Shahsavari Saeed 
Published: February 2022

Abstract

The levels of 6 NDL-PCBs (non-dioxin-like-polychlorinated biphenyls) of were measured in some of Iranian dairy products (yogurt, doogh and kashk). A total of 120 samples were evaluated by modified QuEChERS extraction and gas chromatography-triple-quadrupole mass spectrometry (GC-QqQ-MS/MS) method, also RSD and recovery were determined, 6.33–8.86 and 97.45–102.63 %, respectively. The outcomes of our research exhibited the average levels of Ʃ6-NDL-PCBs in all samples were 15.17±3.44 ng/g fat that were lower than the standard level established by EU (40 ng/g fat). Also, results showed kashk samples had maximum mean level of 6-NDL-PCBs (18.66±2.42 ng/g fat) and doogh samples had minimum mean level of 6-NDL-PCBs (12.21±2.22 ng/g fat). The mean level of 6-NDL-PCBs in yogurt samples was 14.65±2.02 ng/g fat. The heat map results depicted the correlation between spectral indicators 6-NDL-PCBs in different dairy products. A risks assessment based on the Monte Carlo method was done by calculating the Estimated Daily Intake (EDI) and Incremental Life Cancer Risk (ILCR) by the technique of Monte Carlo. The EDI values of 6 NDL-PCBs based on the 95 percentile in yogurt, doogh and kashk were 14.3, 1.49 and 0.5 ng/kg.day, respectively. According to the calculated 95 percent ILCR, dietary exposure to 6 NDL-PCBs does not pose a risk to Iranian consumer health.
The Signature of Glycometabolism-Related Genes in Predicting the Prognosis of Patients with Osteosarcoma
Authors: Wang Fengyan , Yang Kun , Li Ke , Peng Guoxuan , Yang Hua , Xiang Yang , Sun Hong 
Published: February 2022

Abstract
Background
Osteosarcoma is a primary malignant bone tumor with high metastatic potential and an inferior prognosis. Glycometabolism also plays a role in the disease. However, the clinical significance of glycometabolism-related genes in patients with osteosarcoma has been unclear.
Methods
We downloaded the expression profile and corresponding clinical data of osteosarcoma samples from database. Glycometabolism-related gene sets were obtained. Regression analyses were performed to construct a glycometabolism-related prognostic gene signature. The. independent prognostic value of the signature was further assessed by univariate and multivariate Cox regression analysis, and the correlation between immune cells and the signature was investigated. The regulatory mechanism of the prognostic genes was explored by constructing a ceRNA network.
Results
A glycometabolism-related prognostic gene signature based on PRKACB, SEPHS2, GPX7, and PFKFB3 was constructed, and the survival and receiver operating characteristic curves showed that the glycometabolism-related gene signature had a good performance in predicting the overall survival of patients with osteosarcoma. Univariate and multivariate Cox regression analyses confirmed that the glycometabolism-related gene signature was an independent prognostic factor among patients. Correlation analysis revealed that SEPHS2, PRKACB, and GPX7 were correlated with immune cells. A ceRNA network comprised of four genes, 148 miRNAs, and 91 lncRNAs was constructed.
Conclusions
A novel signature based on four glycometabolism-related genes, was constructed, which may facilitate the prognosis and treatment of osteosarcoma in clinical practice.
Fire Simulation and Emergency evacuation simulation of college student apartment
Authors: XIAO Mufeng , ZHOU Xihua , WANG Jue , YU Haibo , HAN Baoxin , GAO Jialu , HAN Yanchun , WANG Yanan 
Published: February 2022

Abstract
The relative concentration of personnel in student apartments in colleges and frequent safety incidents have seriously affected the lives and property safety of students.Therefore, it is of great significance to study the fire in college apartments and its emergency evacuation.Based on the size and volume of No. 4 student dormitory building in the North Campus of Liaoning Technical University,this study use Pyrosim software to carry out fire numerical simulation. The variation rules of smoke visibility, CO concentration, and ambient temperature in the college student apartment building were analyzed and the available safe evacuation time was determined. Moreover, the Pathfinder software was used to carry out emergency evacuation simulation with the combination of physical signs, evacuation speed and personnel proportions, etc.It can analyze the influence of factors such as the number of evacuated persons on each floor of the college student apartment and the layout of the building's staircase on the evacuation time.The result shows:visibility change characteristics have a greater impact on evacuation and rescue;the original layout of the student apartment cannot satisfy the safe evacuation of all personnel;the bottleneck areas for emergency evacuation are indoor corridors and evacuation stairs;increasing the number of evacuation exits in the stairwell can effectively improve the efficiency of escape;when the number of students on each level reaches a certain value, restrictions must be taken, etc.The research results can provide a basis for the reasonable planning and management of evacuation passages in college student apartments.
HEMATOPOIETIC STEM CELL TRANSPLANTATION IN ARPC1B DEFICIENCY
Authors: Giardino Stefano , Volpi Stefano , Lucioni Federica , Caorsi Roberta , Schneiderman Jennifer , Lang Abigail , Khojah Amer , Kuijpers Taco , Papadatou Ionanna , Paisiou Anna , Alonso Laura , Schulz Ansgar , Nufar Marcus , Gattorno Marco , Faraci Maura 
Published: February 2022

Abstract
Mutations in the component isoform ARPC1B of Human actin-related protein 2/3 complex have been recently associated with combined immunodeficiency related to impaired T-cell function, allergies, autoinflammation, and platelets abnormalities. Currently, indications on the management of this novel disease and information on its outcome are lacking. We report the first case series of 7 children who underwent allogeneic-HSCT (allo-HSCT) with homozygous mutation in ARPC1B gene.All patients presented an early clinical onset, complicated with neonatal hemorrhagic enteritis in 3 and macrophage activating syndrome in 2, characterized by recurrent infections, failure to thrive and gastrointestinal bleeding episodes. Allo-HSCT was performed at the median age of 3.54 years after a myeloablative conditioning regimen in all cases. Engraftment occurred in all patients with a full donor chimerism in 6 out of 7. The clinical course after engraftment was uneventful in 3 out of 7 children; two developed a grade 1-2 acute Graft-versus-Host Disease (GvHD), 1 of them a grade 1 chronic-GvHD. Progressive multifocal leukoencephalopathy JC virus-related was diagnosed in one patient 13 months after haploidentical-HSCT, successfully managed with donor-derived viral-specific T-cell infusion. Only one patient had a severe outcome with veno-occlusive disease and transplant-associated microangiopathy and died 3 months after HSCT because of a sepsis. At a median follow-up of 19 months (range 3 – 110), 6 out of 7 patients are alive and disease free.The severe clinical phenotype at diagnosis and the high survival rate with limited transplant-related morbidity reported strongly support the indication to allo-HSCT for patients with this diagnosis.
Speaking COVID-19: Supporting COVID-19 communication and engagement efforts with people from Culturally and Linguistically Diverse Communities
Authors: Seale Holly , Harris-Roxas Ben , Heywood Anita , Abdi Ikram , Mahimbo Abela , Chauhan Ashfaq , Woodland Lisa 
Published: February 2022

Abstract
Background

Since the emergence of COVID-19, issues have been raised regarding the approach used to engage with culturally, and linguistically diverse (CaLD) communities during this public health crisis. This study aimed to understand the factors impacting communication and engagement efforts during the COVID-19 pandemic from the perspective of key CaLD community stakeholders and opinion leaders.
Methods

Forty-six semi-structured telephone interviews were undertaken with key stakeholders who have an active role (established prior to the pandemic) in the delivery of services and other social support to CaLD communities in Australia.
Results
Seven key themes emerged: (1) the digital divide and how to really connect with people; (2) information voids being filled by international material; (3) differentiating established with new and emerging community needs; (4) speaking COVID-19; (5) ineffectiveness of direct translations of English language resources; (6) Coordination is needed to avoid duplication and address gaps and (7) recognising the improvements in governments’ approach.
Conclusion

It is critical that alliances be set up that can be activated in the future to reduce issues around resource development, translation, and dissemination of messages to minimise gaps in the response. Financial assistance must be provided in a timely way to community organisations to support the development and dissemination of culturally appropriate communication materials.
Percutaneous Mesh-container-plasty (PMCP) Versus Percutaneous Kyphoplasty (PKP) in the Treatment of Spinal Metastases: a Retrospective Cohort Study.
Authors: Li Yimin , Qian Yunfan , Shen Guangjie , Tang Chengxuan , Zhong Xiqiang , HE Shaoqi 
Published: February 2022

Abstract
Background: To compare the clinical and radiological results of percutaneous mesh-container-plasty (PMCP) versus percutaneous kyphoplasty (PKP) in the treatment of spinal metastases.Methods: Patients with confirmed diagnosis of spinal metastases and treated in our center between January 2011 and December 2019 were retrospectively included in the study. Ninety-four Patients were divided into 2 groups (PKP 50 and PMCP 44) according the surgical treatment they received. The epidemiological data, surgical outcomes, and clinical and radiological features were compared between the two groups. Cement leakage, height restoration, deformity correction, and cement distribution were calculated from the radiographs. The visual pain analog scale (VAS), Oswestry disability index (ODI), and Karnofsky performance scale (KPS) scores were calculated before surgery, and immediately and 2 years after surgery.Results: The VAS, ODI, and KPS scores improved from 5 (4–6), 65.24±6.59, and 40 (40–50) preoperatively to 2 (1–2), 21.68±4.38, and 70 (70–80) immediately postoperatively in the PKP group (p < 0.05), and from 5 (4–6), 66.45±6.04, and 40 (40–50) preoperatively to 1 (1–2), 21.91±3.88, and 70 (70–80) immediately postoperatively in the PMCP group (p < 0.05), respectively; there was no significant difference between the two groups. The mean cost in the PKP group was lower than that in the PMCP group (5563±439 vs. 6569±344, p < 0.05). Cement distribution in the PMCP group was higher than that in the PKP group (44.30% ± 10.25% vs. 32.54% ± 11.76%, p < 0.05). Cement leakage was observed to be lesser in the PMCP group (7/44) than in the PKP group (18/50, p < 0.05). There were no statistically significant differences in the recovery of vertebral body height and improvement of segmental kyphosis between the PMCP and PKP groups in the treatment of spinal metastases. Conclusions: Both PKP and PMCP have significant abilities in pain relief, functional recovery, height restoration, and segment kyphosis improvement. Despite its higher cost, PMCP may have a better ability to inhibit cement leakage and cement distribution than PKP in the treatment of spinal metastases.
UV-cured coatings for highly efficient passive all-day radiative cooling
Authors: Luo Heng , Yang Meng , Guo Jing , Zou Weizhi , Xu Jian , Zhao Ning 
Published: February 2022

Abstract
Passive radiative cooling provides a promising way to reduce energy consumption for cooling. However, it always requires stringent materials designs or manipulations, which are neither cost-effective nor widely adaptable. Here, we report a fast, inexpensive, and scalable UV-curing method to fabricate porous polymer coatings via photopolymerization-induced phase separation. This coating with hierarchical pore structure, has a superior long-wave infrared emittance of 0.98 and strong solar reflectance of 0.96, resulting in sub-ambient temperature drops of ~9.8°C during the night and ~5.7°C under solar intensity of ~960 W m−2 in the metropolitan area in summer. Moreover, the UV-cured coating is highly crosslinked and superhydrophobic without any treatment, showing excellent durability after long-term UV irradiation, thermal aging, or immersion in corrosive chemicals. The paint-like simplicity and remarkable weather resistance are vital for promoting practical applications of cooling coatings.
Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform
Authors: Naranjo Santiago , Cabana Christina , LaFave Lindsay , Westcott Peter , Romero Rodrigo , Ghosh Arkopravo , Liao Laura , Schenkel Jason , Priore Isabella Del , Jacks Tyler 
Published: February 2022

Abstract
Lung cancer is the leading cause of cancer-related death worldwide. Lung adenocarcinoma (LUAD), the most common histological subtype, accounts for 40% of all cases. While autochthonous, genetically engineered mouse models (GEMMs) recapitulate the histological progression and transcriptional evolution of human LUAD, tumors in these models develop over several months and functional genomic studies are technically challenging. In contrast, cell line transplant models are fast and flexible, but these models fail to capture the full spectrum of disease progression. Organoid technologies provide a means to create next-generation cancer models that integrate the most relevant features of autochthonous and transplant-based systems. However, robust and faithful LUAD organoid platforms are currently lacking. Here, we describe optimized conditions to continuously expand murine alveolar type 2 cells (AT2), a prominent cell-of-origin for LUAD, in organoid culture. These organoids display canonical features of AT2 cells, including marker gene expression, the presence of lamellar bodies, and an ability to differentiate into the AT1 lineage. We used this system to develop flexible and versatile immunocompetent organoid-based models of KRAS, BRAF, and ALK-mutant LUAD. Notably, the resultant tumors closely resemble their autochthonous murine counterparts and human LUAD. In contrast to comparable organoid platforms, our system supports long-term maintenance of the AT2 cellular identity, providing unprecedented ease and reliability to study AT2 and LUAD biology in vitro and in vivo.
Complex Chronic Disease in Pediatrics – Needs in The Last Year of Life
Authors: Nogueira Andreia , Correia Diana , Loureiro Marisa , Gomes Barbara , Cancelinha Cândida 
Published: February 2022

Abstract
Published data on hospital use in the last year of life of a child with a life-threatening complex chronic disease (CCD) is scarce yet critical to inform the implementation phase of paediatric palliative care (PPC). We aimed to describe the last year of life of children with CCD, in terms of the clinic characteristics, hospital resources and the impact of referral to a hospital-based PPC team (PPCT). This is a quantitative, retrospective and comparative study of children with CCD (1–18 years of age), who died in a tertiary hospital between January 2016 and December 2020. We analysed the hospital resources in the last year, therapy and procedures in the last week of life, decision to limit treatment (DLT), place of death, referral to the PPCT and place to death, compared by number of CCDs. 72 patients (60% male) with a median age of 10.1 years were included. Most had ≥ 2 CCDs (58%) with cancer as the most common diagnosis (47%). The group with ≥ 3 CCD had longer hospital stays (p = 0.041). Of the 17 patients referred to the PPCT, there was a higher number of DLT (p < 0.001), greater use of the subcutaneous route (p < 0.001), a lower number of transfusions of blood products (p = 0.002) and a lower number of deaths in the Intensive Care Unit - ICU (p < 0.001). The ICU was the place where the highest number of deaths occurred (64% non-referred vs 6% referred, p < 0.001).Conclusion: Early implementation of PPC optimises the use of hospital resources, minimises invasive procedures and therapies and develops effective and sustainable alternatives which are better suited to the needs of children and/or families.
Genetic variation and population structure analysis of leymus Chinensis from eurasian steppes using SSR makers.
Authors: Ahmed Naseer , Hou Xianyang 
Published: February 2022

Abstract
Leymus chinensis is an essential perennial grass species extensively dispersed in the typical grassland communities of the Eurasian steppe region. It is relished by livestock due to its high quality and being a nutritionally valuable forage crop. Leymus chinensis has recently gained extensive consideration on its genetic diversity. However, genetic diversity studies on L. Chinensis using SSR markers is currently limited. In the present study, we investigate the genetic variation and population structure analysis of leymus chinensis from Eurasian steppes using SSR makers. For the genetic diversity, nineteen SSR markers were used and a total of 133 alleles were identified across the 166 L. Chinensis plants. Our findings illustrated that the polymorphic rate for all SSR markers was greater than 80%, with the exception of SSR12i and SSR6c, which had polymorphism rates of 50% and 75%, respectively. The gene diversity (H) ranged from 0.0545 for SSR12i to 0.4720 for SSR25v, and the average was 0.3136. Furthermore, genetic diversity analysis indicated that the 166 samples could be grouped into five main population clusters based on their maximum membership coefficients which were assigned as Pop1 to Pop5. Among the five populations, the largest values of allele (total number of detected alleles), Ne (effective number of alleles) and Na (observed mean number of alleles) was found to be higher in Pop1, with values of 61, 1.461, and 1.977, respectively. Additionally, AMOVA showed that 13% of the total genetic variation occurred among the population and 87% genetic variation within the populations of the species. Whereas, the pairwise Fst specified the moderate genetic variation ranging from 0.0336 to 0.0731. Finally, the principal coordinate analysis revealed that the x-axis and y-axis explained 5.72% and 4.86% of the variation in molecular data, respectively. Taken together, these SSR markers provide new insights for a more precise understanding of the genetic diversity of L. Chinensis germplasm and could potentially enhance the breeding program of L. Chinensis.
Diedaqili Tablet Promotes Bony Fusion Accelerates Fracture Healing In Mice
Authors: Zhao Ye , Zhang Yujie , Liu Kaoqiang , Yao Shen , Chai Yongli , Zhan Hongsheng , Yuan Weian , Shen Zhibi 
Published: February 2022

Abstract
Diedaqili tablet has been found effective for fracture healing in previous clinical studies. In a recent study, we investigated the effects of diedaqili tablet (DDQL) on bone fracture repair in mice. Adult C57BL/6 mice were subjected to transverse femoral fractures and administrated orally with a low dose (40.55mg/ ml), a high dose (162.18mg/ml) DDQL suspension and normal saline daily from day 1 after operation. The femur and blood of mice was analyzed by plain radiography, micro-computed tomography (Micro-CT), histology, biomechanical analysis, and serum Ca, P, and ALP test. The results demonstrated that DDQL can effectively improve the porosis of fracture end and bony union in the course of healing via Micro-CT and hematoxylin-eosin staining (HE staining) analysis. Consistent with morphological findings, biomechanical properties of fracture healing have also been demonstrated. And Diedaqili tablet has a dose-dependent effect. DDQL augmented the release of alkaline phosphatase (ALP) and phosphorus (P) into blood, indicating that it promoted mineralization of hypertrophic cartilage and woven bone growth simultaneously during bone healing. In summary, the preliminary experiment revealed that DDQL can improve bone formation via promoting osteogenic capability, calcium (Ca) and P metabolism, and subsequently accelerates fracture repair and bony fusion.
Exploring the Potential Mechanism of Zhengan Xifeng Decoction for ischemic stroke based on Network pharmacology and Molecular Docking
Authors: Feng Xiaodong , Zhang Yasu , liu xiaomin , Long Junzi , Gao Qian , Pan Mengyang 
Published: February 2022

Abstract
Backgroud Ischemic stroke (IS) is an acute cerebrovascular incident that threatens public health. Zhengan Xifeng Decoction (ZXD), as a common herbal formula, has been widely applied in clinical practice for IS. The purpose of this study was to investigate the bioactive ingredients and potential pharmacological mechanisms of ZXD for IS based on network pharmacology and molecular docking.Methods Chemical ingredients of ZXD were screened from TCMSP, BATMAN-TCM and the literature. Then, the targets of the chemical ingredients were predicted through STITCH and SwissTargetPrediction databases. IS-related targets were selected from DisGeNET, DrugBank, MalaCards and GeneCards databases. And, the String database and Cytoscape 3.8.2 software were used to construct the protein-protein interaction (PPI) network. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analysis were analyzed in Metascape. At last, Molecular docking was carried out using Open Babel GUI, AutoDock 1.5.6 and Pymol.Results 284 active ingredients involving 2353 putative targets were identified in ZXD, of which 1098 targets were linked to IS. A total of 20 core targets including MAPK3, MAPK1, and AKT1 were identified by analyzing PPI network. The biological process associated with IS were related to response to growth factor, apoptotic signaling pathway, MAPK cascade and neuron death. And KEGG enrichment included that Prolactin signaling pathway, VEGF signaling pathway and ErbB signaling pathway were closely correlated with neurogenesis, neurotransmission, synaptic plasticity vascular growth factor regulation, in IS. Finally, molecular docking revealed that core ingredients of Quercetin, Carvone and Asparamide had well-binding ability to core targets.Conclusion The study suggested that ZXD treated IS by modulating multiple targets and pathways. And, the results demonstrated that the mechanism of ZXD anti-IS may be related to the regulation of neuroprotection and angiogenesis through Prolactin signaling pathway, VEGF signaling pathway and the ErbB signaling pathway.