bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of the COVID‐19 pandemic on the glycemic control, eating habits, and body compositions of people with diabetes mellitus: A retrospective longitudinal observational study
Authors: Sawada Mika , Ohkuma Kanako , Aihara Masakazu , Doi Shunsuke , Sekine Rie , Kaneko Tetsuji , Iimuro Satoshi , Ichi Ikuyo , Usami Satoshi , Ohe Kazuhiko , Yamauchi Toshimasa , Kubota Naoto 
Published: November 2022

ABSTRACTAims/IntroductionTo evaluate the impact of the COVID‐19 pandemic on the glycemic control, eating habits, and body composition of people with diabetes mellitus; to identify the determinants of worsening glycemic control in people with diabetes mellitus.Materials and methodsThis retrospective, longitudinal observational study was performed in outpatients with diabetes mellitus who visited our hospital between April 2019 and March 2020 (pre‐COVID‐19 period) and continued for follow up from April 2020 to March 2021 (COVID‐19 period). We compared the glycemic control, nutritional intakes, and body composition of people with diabetes mellitus between the two periods. The changes in the HbA1c values (ΔHbA1c) and other study variables were compared between the two periods. Logistic regression analysis was performed to identify the factors associated with the increase of HbA1c levels.ResultsA significant increase of HbA1c was observed during the COVID‐19 period. The percent fat mass (FM) also increased, while the percent skeletal muscle mass (SMM) decreased during the COVID‐19 period. After adjustments for age and sex, the ΔBMI (OR:2.33), ΔFM (OR:1.45), and ΔSMM (OR:0.51) were identified as being associated with elevated levels of HbA1c.ConclusionsThe COVID‐19 pandemic had a negative impact on the glycemic control and body composition of people with diabetes mellitus. The increased body weight and FM and decreased SMM observed during the pandemic were associated with poor glycemic control in people with diabetes mellitus.
Changes of HbA1c variability after the switch to a longer‐acting insulin analog in people with type 1 diabetes
Authors: Watanabe Hirotaka , Takahara Mitsuyoshi , Katakami Naoto , Shimomura Iichiro 
Published: November 2022

ABSTRACTThis study investigated whether longer‐acting basal analogs (insulin degludec and insulin glargine U300) could reduce visit‐to‐visit hemoglobin A1c (HbA1c) variability in patients with type 1 diabetes. Ninety adults with type 1 diabetes for whom the basal insulin was switched to a longer‐acting insulin analog were analyzed retrospectively. The coefficient of variation of HbA1c levels (CV‐HbA1c) during the year before and after the switch was compared. The CV‐HbA1c after the switch was not significantly different from that before the switch (4.39 ± 2.24% vs 4.25 ± 2.07%; P = 0.506). The linear regression model revealed that CV‐HbA1c before the switch was independently associated with the change of CV‐HbA1c (regression coefficient per standard deviation = −0.568, P < 0.001), whereas the other variables were not (all P > 0.05). In conclusion, CV‐HbA1c remained unchanged after the switch on average, but CV‐HbA1c before the switch was associated with the decrease of CV‐HbA1c in individuals with type 1 diabetes.
Evaluation of insecticidal properties of botanicals for sustainable management of sucking pests of horticultural crops
Authors: Prasannakumar Nagalapura Ramakrishnappa , Rao Vala Keshava , Jyothi Nagendraiah , Saroja Sarikonda , Lokesha Ankanahalli Naranayanashetty , Ramkumar Govindaraju 
Published: November 2022

AbstractAphids, thrips, whiteflies, mealy bugs and mites could cause significant yield losses in horticultural crops. Many synthetic chemicals highly hazardous to non‐target organisms and the environment are in use in their management. Botanicals are ideal for safe sustainable pest management by keeping synthetic chemical use at a minimum. We studied the insecticidal properties of three botanicals viz., Annona squamosa, Ricinus communis and Sapindus mukorossi seed extracts against sucking pests viz., aphids (Aphis gossypii and Aphis craccivora) on okra (Abelmoschus esculentus) and bittergourd (Momordica charantia), thrips (Scirtothrips dorsalis) on chilli (Capsicum frutescens), whiteflies (Bemisia tabaci) on eggplant (Solanum melongena), mealy bugs (Maconellicoccus hirsutus) on betel vine (Piper betle) and mites (Tetranychus urticae) on eggplant (Solanum melongena) under laboratory conditions. The bioassay showed that A. squamosa seed extract has potent insecticidal properties on all sucking pests, except T. urticae. Though S. mukorossi seed extract exhibited no insecticidal property, had a significant acaricidal property. A. squamosa and S. mukorossi seed extracts will therefore be additional botanicals for use in organic farming for managing the sucking pests effectively.
Serum amyloid A1 recruits neutrophils to the invasive front of T1 colorectal cancers
Authors: Yoshido Ayano , Sudo Gota , Takasawa Akira , Aoki Hironori , Kitajima Hiroshi , Yamamoto Eiichiro , Niinuma Takeshi , Harada Taku , Kubo Toshiyuki , Sasaki Hajime , Ishiguro Kazuya , Yorozu Akira , Kai Masahiro , Katanuma Akio , Yamano Hiro‐o , Osanai Makoto , Nakase Hiroshi , Suzuki Hiromu 
Published: November 2022

AbstractBackground and AimThe tumor microenvironment plays an essential role in the development and progression of colorectal cancer (CRC). We recently reported that crosstalk between CRC cells and tumor‐associated macrophages (TAMs) via serum amyloid A1 (SAA1) promotes invasion by T1 CRCs. In the present study, we aimed to clarify the role of neutrophils in early CRCs.MethodsImmunohistochemical analysis of CD66b, chemokine CXC motif ligand 8 (CXCL8 or interleukin‐8, IL‐8) and matrix metalloproteinase‐9 (MMP‐9) was performed using primary T1 CRCs (n = 49). The HL‐60 human promyelocytic leukemia cell line and THP‐1 human monocytic leukemia cell line were used to obtain neutrophil‐like and macrophage‐like cells, respectively. Boyden chamber assays were used to analyze cell migration and invasion, and quantitative RT‐PCR was used to analyze gene expression.ResultsImmunohistochemical analysis revealed accumulation of neutrophils at the SAA1‐positive invasive front of T1 CRCs. Experiments using HL‐60 cells suggested that treatment with SAA1 induced neutrophil migration and expression of CXCL8 and MMP‐9 in neutrophils and that neutrophils promote CRC cell migration and invasion. Immunohistochemistry confirmed accumulation of CXCL8‐ or MMP‐9‐positive neutrophils at the SAA1‐positive invasive front of T1 CRCs. Moreover, co‐culture experiments using CRC, THP‐1 and HL‐60 cells suggested that CRC cells activated by macrophages upregulate CXCL8 and MMP‐9 in neutrophils.ConclusionsOur results suggest that interplay between macrophages and CRC cells leads to recruitment of neutrophils to the invasive front of T1 CRCs and that SAA1 secreted by CRC cells activate neutrophils to promote invasion.
Effects of moxibustion on cognition and activities of daily living in post‐stroke cognitive impairment: A systematic review and meta‐analysis of randomized controlled trials
Authors: Liu Fang , Lyu Zecai , Lin Shaohong , Li Zhuangmiao , Xiu Huoqin 
Published: November 2022

AbstractBackgroundPost‐stroke cognitive impairment (PSCI) imposes a huge burden on patients and society as a whole; however, unequivocally effective treatments for PSCI are still lacking. Therefore, the exploration of effective and safe non‐pharmacological treatment modalities for PSCI is a key imperative. Moxibustion has been widely used for cognitive rehabilitation; however, there is a paucity of systematic reviews of the available evidence. Therefore, we conducted a systematic review and meta‐analysis of randomized controlled trials (RCTs) that investigated the effectiveness of moxibustion for treatment of PSCI to provide evidence base for the treatment of PSCI with moxibustion.ObjectiveTo evaluate the efficacy of moxibustion in improving cognitive function and activities of daily living (ADLs) in patients with PSCI.DesignSystematic review and meta‐analysis of RCTs.ParticipantsPatients with a clinical diagnosis of PSCI.Review MethodsRelevant studies published in English or Chinese were retrieved from ten databases until December 2021. RCTs that assessed the efficacy of moxibustion on cognitive functioning and ADL in patients with PSCI were included. Two reviewers independently identified the trials and extracted the data. Risk‐of‐bias was assessed using the Cochrane Risk of Bias Tool. Cochrane's Review Manager (RevMan 5.4) software was used for the meta‐analysis.ResultsEighteen RCTs (1290 participants) qualified the inclusion criteria and were included. Compared with the control group, the addition of moxibustion significantly improved the cognitive function, evaluated using the Montreal Cognitive Assessment (MoCA) [pooled mean difference (MD): 2.27, 95% CI: 1.98, 2.55, I2 = 22%]. The pooled MD of Mini‐Mental State Examination (MMSE) score was 1.85 (95% CI: 1.56, 2.15, I2 = 26%), and the pooled odds ratios (OR) total effective rate was 4.74 (95% CI: 2.55, 8.80, I2 = 0%) (p < 0.05 for all). Moxibustion also significantly improved ADL, assessed using Modified Barthel Index (MBI) (pooled MD = 4.10, 95% CI: 2.10 to 6.10, I2 = 0%) and Barthel Index (pooled MD: 8.63, 95% CI: 7.47, 9.79, I2 = 5%) (p < 0.05 for all).ConclusionsCompared with control group, the addition of moxibustion significantly improved the cognition and ADL of patients with PSCI.Clinical RelevanceNurses can incorporate moxibustion into the rehabilitation nursing of PSCI.
Enabling professional and personal growth among home care nurses through using the Carer Support Needs Assessment Tool Intervention—An interpretive descriptive study
Authors: Norinder Maria , Axelsson Lena , Årestedt Kristofer , Grande Gunn , Ewing Gail , Alvariza Anette 
Published: November 2022

AbstractAimTo explore nurses' experiences of supporting family caregivers in specialised home care while learning to use the Carer Support Needs Assessment Tool Intervention.BackgroundThe Carer Support Needs Assessment Tool Intervention can provide guidance for discussions with family caregivers in specialised home care concerning their specific support needs. Little attention has been paid to how nurses experience the use of the intervention in their everyday practice.DesignThis longitudinal study adopted an inductive qualitative approach using interpretive description.MethodsInterviews were conducted at two time points. A total of 22 interviews took place with 12 nurses recruited from six specialised home care services. Data were analysed using interpretive description.ResultsNurses' everyday clinical practice changed while learning to use the Carer Support Needs Assessment Tool Intervention, and they experienced professional and personal growth. Their supportive inputs shifted from being reactive towards being more proactive. Their approach changed from taking on great professional responsibility, towards a shared responsibility with family caregivers. The support altered from ad hoc contacts in the hallway, towards scheduled trustful conversations. Nurses were concerned about the amount of time and energy this kind of support might require. They pointed to the importance of holding good nursing skills to conduct this new way of having conversations.ConclusionNurses' everyday clinical practice can be further developed through the use of the Carer Support Needs Assessment Tool Intervention. Nurses may develop both professionally and personally, increasing their ability to provide person‐centred support.Relevance to Clinical PracticeWith the use of the Carer Support Needs Assessment Tool Intervention, nurses can create trusting conversations with family caregivers of patients with life‐threatening illnesses cared for in specialised home care.Reporting MethodReporting of the study follows the Consolidated Criteria For Reporting Qualitative Research (COREQ) checklist (File S1).Patient or Public ContributionParticipating nurses were involved in discussing the study design.
Regulation of osteogenesis in bone marrow‐derived mesenchymal stem cells via histone deacetylase 1 and 2 co‐cultured with human gingival fibroblasts and periodontal ligament cells
Authors: Iwata Tomoyuki , Kaneda‐Ikeda Eri , Takahashi Keita , Takeda Katsuhiro , Nagahara Takayoshi , Kajiya Mikihito , Sasaki Shinya , Ishida Shu , Yoshioka Minami , Matsuda Shinji , Ouhara Kazuhisa , Fujita Tsuyoshi , Kurihara Hidemi , Mizuno Noriyoshi 
Published: November 2022

AbstractObjectiveThis study aimed to determine the regulatory mechanism of bone marrow‐derived mesenchymal stem cell (BM‐MSC) differentiation mediated by humoral factors derived from human periodontal ligament (HPL) cells and human gingival fibroblasts (HGFs). We analyzed histone deacetylase (HDAC) expression and activity involved in BM‐MSC differentiation and determined their regulatory effects in co‐cultures of BM‐MSCs with HPL cells or HGFs.BackgroundBM‐MSCs can differentiate into various cell types and can, thus, be used in periodontal regenerative therapy. However, the mechanism underlying their differentiation remains unclear. Transplanted BM‐MSCs are affected by periodontal cells via direct contact or secretion of humoral factors. Therefore, their activity is regulated by humoral factors derived from HPL cells or HGFs.MethodsBM‐MSCs were indirectly co‐cultured with HPL cells or HGFs under osteogenic or growth conditions and then analyzed for osteogenesis, HDAC1 and HDAC2 expression and activity, and histone H3 acetylation. BM‐MSCs were treated with trichostatin A, or their HDAC1 or HDAC2 expression was silenced or overexpressed during osteogenesis. Subsequently, they were evaluated for osteogenesis or the effects of HDAC activity.ResultsBM‐MSCs co‐cultured with HPL cells or HGFs showed suppressed osteogenesis, HDAC1 and HDAC2 expression, and HDAC phosphorylation; however, histone H3 acetylation was enhanced. Trichostatin A treatment remarkably suppressed osteogenesis, decreasing HDAC expression and enhancing histone H3 acetylation. HDAC1 and HDAC2 silencing negatively regulated osteogenesis in BM‐MSCs to the same extent as that achieved by indirect co‐culture with HPL cells or HGFs. Conversely, their overexpression positively regulated osteogenesis in BM‐MSCs.ConclusionThe suppressive effects of HPL cells and HGFs on BM‐MSC osteogenesis were regulated by HDAC expression and histone H3 acetylation to a greater extent than that mediated by HDAC activity. Therefore, regulation of HDAC expression has prospects in clinical applications for effective periodontal regeneration, mainly, bone regeneration.
Influence of social jetlag on daytime sleepiness in obstructive sleep apnea
Authors: Jin Charley Ximing , Sutherland Kate , Gislason Thorarinn , Thorarinsdottir Elin Helga , Bittencourt Lia , Tufik Sergio , Singh Bhajan , McArdle Nigel , Cistulli Peter , Bin Yu Sun 
Published: November 2022

SummarySocial jetlag is the discrepancy between socially determined sleep timing on workdays and biologically determined sleep timing on days free of social obligation. Poor circadian timing of sleep may worsen sleep quality and increase daytime sleepiness in obstructive sleep apnea (OSA). We analysed de‐identified data from 2,061 participants (75.2% male, mean [SD] age 48.6 [13.4] years) who completed Sleep Apnea Global Interdisciplinary Consortium (SAGIC) research questionnaires and underwent polysomnography at 11 international sleep clinic sites. Social jetlag was calculated as the absolute difference in the midpoints of sleep between weekdays and weekends. Daytime sleepiness was assessed using the Epworth Sleepiness Scale (ESS). Linear regression analyses were performed to estimate the association between social jetlag and daytime sleepiness, with consideration of age, sex, body mass index, ethnicity, insomnia, alcohol consumption, and habitual sleep duration as confounders. Of the participants, 61.5% had <1 h of social jetlag, 27.5% had 1 to <2 h, and 11.1% had ≥2 h. Compared to those with <1 h of social jetlag, those with ≥2 h of social jetlag had 2.07 points higher ESS (95% confidence interval [CI] 0.77–3.38, p = 0.002), and those with 1 to <2 h of social jetlag had 0.80 points higher ESS (95% CI 0.04–1.55, p = 0.04) after adjustment for potential confounding. Interaction with OSA severity was observed; social jetlag appeared to have the greatest effect on daytime sleepiness in mild OSA. As social jetlag exacerbates daytime sleepiness in OSA, improving sleep timing may be a simple but novel therapeutic target for reducing the impact of OSA.
Prayer in America: A Detailed Analysis of the Various Dimensions of Prayer
Authors: Froese Paul , Uecker Jeremy E. 
Published: November 2022

AbstractDespite prayer's central role in religious life, quantitative research on prayer typically focuses on its frequency without regard to the total amount, to why, to whom, or for what a person might be praying, nor how people feel when they pray. In this paper, we broaden our understanding of the prayer practices of American adults by focusing on their (1) quantity of prayer, (2) style of prayer, (3) purpose of prayer, (4) prayer target, and (5) emotional state during prayer. Using the Baylor Religion Survey (N = 1248), collected January–March 2021, we present frequencies and findings from factor analyses of key conceptual dimensions of prayer and then indicate how prayer dimensions correlate with each other and vary by basic religion and demographic variables. Our findings provide a detailed picture of these relationships and also provide researchers with best practices in how to measure the various dimensions of prayer in future studies.
Myeloid cell subpopulations compensate each other for Ccr2‐deficiency in glioblastoma
Authors: Bungert Alexander D. , Urbantat Ruth M. , Jelgersma Claudius , Bekele Biniam M. , Mueller Susanne , Mueller Annett , Felsenstein Matthäus , Dusatko Silke , Blank Anne , Ghori Adnan , Boehm‐Sturm Philipp , Koch Stefan P. , Vajkoczy Peter , Brandenburg Susan 
Published: November 2022

AbstractAimsGlioblastomas are high‐grade brain tumours that are characterised by the accumulation of brain‐resident microglia and peripheral macrophages. Recruitment of these myeloid cells can be facilitated by CCR2/CCL2 signalling. Besides the well‐known CCR2+ macrophages, we have identified microglia expressing CCR2 in glioma tissues. Thus, we investigated how Ccr2‐deficiency of one of the myeloid cell populations affects the other population and tumour biology.MethodsWe generated four chimeric groups to analyse single and combined Ccr2‐deficiency of microglia and macrophages. On day 21 after tumour cell implantation (GL261), we conducted flow cytometry, immunofluorescence and real‐time polymerase chain reaction analyses. Tumour volume and metabolism were determined by magnetic resonance imaging and magnetic resonance spectroscopy. Moreover, in vitro studies were performed with primary microglia and bone marrow‐derived macrophages.ResultsWe demonstrated reduced infiltration of macrophages and microglia depending on the lack of Ccr2. However, the total number of myeloid cells remained constant except for the animals with dual Ccr2‐knockout. Both microglia and macrophages with Ccr2‐deficiency showed impaired expression of proinflammatory molecules and altered phagocytic activity. Despite the altered immunologic phenotype caused by Ccr2‐deficiency, glioma progression and metabolism were hardly affected. Alterations were detected solely in apoptosis and proliferation of tumours from animals with specific Ccr2‐deficient microglia, whereas vessel stability was increased in mice with Ccr2‐knockout in both cell populations.ConclusionThese results indicate that microglia and macrophages provide a homoeostatic balance within glioma tissue and compensate for the lack of the corresponding counterpart. Moreover, we identified that the CCR2/CCL2 axis is involved in the immunologic function of microglia and macrophages beyond its relevance for migration.
Neurophysiological mechanisms of gait disturbance in advanced Parkinson's disease patients
Authors: Takakusaki Kaoru , Takahashi Mirai , Noguchi Tomohiro , Chiba Ryosuke 
Published: November 2022

AbstractThis review considers pathophysiological mechanisms of posture‐gait disturbances in Parkinson's disease (PD). Clinical studies have shown that posture‐gait disturbance attributes to the dysfunction of the whole neuraxis in addition to the musculoskeletal system. The cerebral cortex, basal ganglia (BG), cerebellum, brainstem, and spinal cord temporally and spatially integrate and coordinate multisensory feedback and efferent copies of the motor command. Therefore, the extensive repertoire of voluntary movements can be coupled with anticipatory and reactive postural adjustments to provide the framework for supporting and stabilizing the goal‐directed gait activity. Redundancies in the system allow adaptation and compensation through reward‐oriented and error‐based learning processes implemented through the BG and cerebellar pathways, respectively. However, the impairment of these systems in PD may considerably compromise the capacity to adapt and lead to maladaptive changes impairing posture‐gait control. When these impairments occur, the risk of falls can significantly increase, and interventions are required to reduce morbidity. The damage in dopamine (DA) neurons is the primary cause of PD. Insufficient DA supply in the striatum disturbs the operation of intrinsic networks in the BG. This also pathologically increases GABAergic BG output to the cerebral cortex and brainstem, resulting in functional disconnection of other structures from the BG. The disconnection makes PD patients disable to achieve habitually acquired automatized gait control. Moreover, Lewy body degeneration in most brain areas, particularly in cholinergic and other monoaminergic systems vulnerable to neurodegeneration, further disturbs posture‐gait control and alters non‐motor symptoms of PD.
Maize lateral rootless 1 encodes a homolog of the DCAF protein subunit of the CUL4‐based E3 ubiquitin ligase complex
Authors: Baer Marcel , Taramino Graziana , Multani Dilbag , Sakai Hajime , Jiao Shuping , Fengler Kevin , Hochholdinger Frank 
Published: November 2022

Summary

In maize (Zea mays L.), lateral roots are formed in the differentiation zone of all root types in a multi‐step process. The maize mutant lateral rootless 1 (lrt1) is defective in lateral root formation in primary and seminal roots but not in shoot‐borne roots.

We cloned the lrt1 gene by mapping in combination with BSA‐seq and subsequent validation via CRISPR/Cas9.

The lrt1 gene encodes a 209 kDa homolog of the DDB1‐CUL4‐ASSOCIATED FACTOR (DCAF) subunit of the CUL4‐based E3 ubiquitin ligase (CRL4) complex localized in the nucleus. DDB1‐CUL4‐ASSOCIATED FACTOR proteins are encoded by an evolutionary old gene family already present in nonseed plants. They are adaptors that bind substrate proteins and promote their ubiquitylation, thus typically marking them for subsequent degradation in the 26S proteasome. Gene expression studies demonstrated that lrt1 transcripts are expressed preferentially in the meristematic zone of all root types of maize. Downregulation of the rum1 gene in lrt1 mutants suggests that lrt1 acts upstream of the lateral root regulator rum1.

Our results demonstrate that DCAF proteins play a key role in root‐type‐specific lateral root formation in maize. Together with its role in nitrogen acquisition in nitrogen‐poor soil, lrt1 could be a promising target for maize improvement.