bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leaf starch metabolism sets the phase of stomatal rhythm
Authors: Westgeest Adrianus J. , Dauzat Myriam , Simonneau Thierry , Pantin Florent 
Published: October 2022

ABSTRACTIn leaves of C3and C4plants, stomata open during the day to favour CO2entry for photosynthesis, and close at night to prevent inefficient transpiration of water vapour. The circadian clock paces rhythmic stomatal movements throughout the diel (24-h) cycle. Leaf transitory starch is also thought to regulate the diel stomatal movements, yet the underlying mechanisms across time (key moments) and space (relevant leaf tissues) remains elusive. Here, we developed PhenoLeaks, a pipeline to analyse the diel dynamics of transpiration, and used it to screen a series of Arabidopsis mutants impaired in starch metabolism. We detected a sinusoidal, endogenous rhythm of transpiration that overarches days and nights. We uncovered that a number of severe mutations in starch metabolism affect the endogenous rhythm through a phase shift, resulting in delayed stomatal movements throughout the daytime and reduced stomatal preopening during the night. Nevertheless, analysis of tissue-specific mutations revealed that neither guard-cell nor mesophyll-cell starch metabolism are strictly required for normal diel patterns of transpiration. We propose that leaf starch influences the timing of transpiration rhythm through an interplay between the clock and sugars across tissues, while the energetic effect of starch-derived sugars is usually non-limiting for endogenous stomatal movements.One-sentence summaryThe PhenoLeaks pipeline for monitoring diel transpiration dynamics reveals that leaf starch metabolism sets the timing of the endogenous stomatal rhythm.
Genetic diversity and structure in wild Robusta coffee (Coffea canephora A. Froehner) populations in Yangambi (DR Congo) and their relation with forest disturbance
Authors: Depecker Jonas , Verleysen Lauren , Asimonyio Justin A , Hatangi Yves , Kambale Jean-Léon , Mwanga Mwanga Ithe , Tshimi Ebele , Dhed’a Benoit , Bawin Yves , Staelens Ariane , Stoffelen Piet , Ruttink Tom , Vandelook Filip , Honnay Olivier 
Published: October 2022

AbstractDegradation and regeneration of tropical forests can strongly affect gene flow in understorey species, resulting in genetic erosion and changes in genetic structure. Yet, these processes remain poorly studied in tropical Africa. Coffea canephora is an economically important species, found in the understorey of tropical rainforests of Central and West Africa, and the genetic diversity harboured in its wild populations is vital for sustainable coffee production worldwide. Here, we aimed to quantify genetic diversity, genetic structure, and pedigree relations in wild C. canephora populations, and we investigated associations between these descriptors and forest disturbance and regeneration. Therefore, we sampled 256 C. canephora individuals within 24 plots across three forest categories in Yangambi (DR Congo), and used genotyping-by-sequencing to identify 18 894 SNPs. Overall, we found high genetic diversity, and no evidence of genetic erosion in C. canephora in disturbed old-growth forest, as compared to undisturbed old-growth forest. Additionally, an overall heterozygosity excess was found in all populations, which was expected for a self-incompatible species. Genetic structure was mainly a result of isolation-by-distance, reflecting geographical location, with low to moderate relatedness at finer scales. Populations in regrowth forest had lower allelic richness than populations in old-growth forest and were characterised by a lower inter-individual relatedness and a lack of isolation-by-distance, suggesting that they originated from different neighbouring populations and were subject to founder effects. Wild Robusta coffee populations in the study area still harbour high levels of genetic diversity, yet careful monitoring of their response to ongoing forest degradation remains required.
Development of SARS-CoV-2 mRNA vaccines encoding spike N-terminal and receptor binding domains
Authors: Stewart-Jones Guillaume B.E. , Elbashir Sayda M. , Wu Kai , Lee Diana , Renzi Isabella , Ying Baoling , Koch Matthew , Sein Caralyn E. , Choi Angela , Whitener Bradley , Garcia-Dominguez Dario , Henry Carole , Woods Angela , Ma LingZhi , Montes Berrueta Daniela , Avena Laura E. , Quinones Julian , Falcone Samantha , Hsiao Chiaowen J. , Scheaffer Suzanne M. , Thackray Larissa B. , White Phil , Diamond Michael S. , Edwards Darin K. , Carfi Andrea 
Published: October 2022

AbstractWith the success of mRNA vaccines against coronavirus disease 2019 (COVID-19), strategies can now focus on improving vaccine potency, breadth, and stability. We present the design and preclinical evaluation of domain-based mRNA vaccines encoding the wild-type spike-protein receptor-binding (RBD) and/or N-terminal domains (NTD). An NTD-RBD linked candidate vaccine, mRNA-1283, showed improved antigen expression, antibody responses, and stability at refrigerated temperatures (2-8°C) compared with the clinically available mRNA-1273, which encodes the full-length spike protein. In mice administered mRNA-1283 as a primary series, booster, or variant-specific booster, similar or greater immune responses and protection from viral challenge were observed against wild-type, beta, delta, or omicron (BA. 1) compared with mRNA-1273 immunized mice, especially at lower vaccine dosages. These results support clinical assessment of mRNA-1283 (NCT05137236).One Sentence SummaryA domain-based mRNA vaccine, mRNA-1283, is immunogenic and protective against SARS-CoV-2 and emerging variants in mice.
Lack of accelerated ovarian aging in a follicle-stimulating hormone receptor haploinsufficiency model
Authors: Mehalko Kristen , Kim Minhoo , Paye Sanjana , Koh Kelly , Lu Ryan J. , Benayoun Bérénice A. 
Published: October 2022

AbstractFollicle-stimulation hormone (FSH) and FSH receptor (FSHR) signaling is essential for lifelong ovarian and endocrine functions in females. Previous studies have reported thatFshrhaploinsufficiency in female mice led to accelerated ovarian aging, including anticipated progressive fertility decline, irregular estrus cycles, increased follicular atresia and premature ovarian failure at 7 to 9 months of age. Interestingly, these phenotypes resemble key characteristics of human menopause and thusFshrhaploinsufficiency was proposed as a promising research mouse model of menopause. However, theFshrhaploinsufficiency model had not been fully explored, especially at the molecular level. In this study, we characterized the ovarian and endocrine functions of aFshrheterozygous knockout allele that was generated on the C57BL/6 genetic background as part of the Knockout Mouse Project (KOMP). Based on our analyses of these mice using a breeding assay, ovarian tissue histology and serum hormone quantifications (i.e. FSH, AMH, INHA) analyses, the KOMPFshrheterozygous knockout female mice do not show the anticipated phenotypes of ovarian aging in terms of fertility and endocrine function. We further confirmed that the expression ofFshris unaltered in the ovaries of the KOMPFshrheterozygous knockout animals compared to wild-type. Together, our data suggests that the KOMPFshrheterozygous knockout strain does not recapitulate the previously reported ovarian aging phenotypes associated to another model ofFshrhaploinsufficiency.
Fate and plasticity of SARS-CoV-2-specific B cells during memory and recall response in humans
Authors: Zurbuchen Yves , Michler Jan , Taeschler Patrick , Adamo Sarah , Cervia Carlo , Raeber Miro E. , Acar Ilhan E. , Nilsson Jakob , Soyka Michael B. , Moor Andreas E. , Boyman Onur 
Published: October 2022

AbstractB cell responses to different pathogens recruit tailored effector mechanisms, resulting in functionally specialized subsets. For human memory B cells (MBCs), these include CD21+ resting, CD21−CD27+ activated, and CD21−CD27− atypical cells. Whether these subsets follow deterministic or interconnected fates is unknown. We demonstrate in COVID-19 patients that single clones of SARS-CoV-2-specific MBCs followed multiple fates with distinctive phenotypic and functional characteristics. 6–12 months after infection, most circulating MBCs were CD21+ resting cells, which also accumulated in peripheral lymphoid organs where they acquired markers of tissue residency. Conversely, at acute infection and following SARS-CoV-2-specific immunization, CD21− MBCs became the predominant subsets, with atypical MBCs expressing high T-bet, inhibitory molecules, and distinct chemokine receptors. B cell receptor sequencing allowed tracking of individual MBC clones differentiating into CD21+, CD21−CD27+, and CD21−CD27− cell fates. Collectively, single MBC clones can adopt functionally different trajectories, thus contributing to immunity to infection.
Interrelations of slenderness ratio and main design criteria in supertall buildings
Authors: Ilgın Hüseyin Emre 
Published: October 2022

PurposeTo date, there are no studies in the literature that provide a comprehensive understanding of the interrelationships between the slenderness ratio and the main design criteria in supertall towers (=300 m). In this paper, this important issue was explored using detailed data collected from 75 cases.Design/methodology/approachThis paper was carried out with a comprehensive literature review including the database of the Council on Tall Buildings and Urban Habitat(CTBUH) (CTBUH, 2022), peer-reviewed journals, MSc theses and PhD dissertations, conference proceedings, fact sheets, architectural and structural magazines and other Internet sources. In this study, the case study method was also used to gather and consolidate information about supertall towers to analyze the interrelationships. Cases were 75 supertall buildings in various countries [44 from Asia (37 from China), 16 from the Middle East (6 from Dubai, the United Arab Emirates), 11 from the United States of America and 3 from Russia, 1 from the UK].FindingsThe paper's findings highlighted as follows: (1) for buildings in the height range of 300–399 m, the slenderness ratio was usually between 7 and 7.9 and megatall towers were frequently built at a slenderness ratio of 10–15; (2) the median slenderness ratio of buildings in the 400–599 m height ranges was around 8.6; (3) a trend towards supertall slender buildings (=8) was observed in Asia, the Middle East and North America; (4) residential, office and mixed-use towers had a median slenderness ratio of over 7.5; (5) all building forms were utilized in the construction of slender towers (>8); (6) the medium slenderness ratio was around 8 for supertall buildings constructed with outriggered frame and tube systems; (7) especially concrete towers reached values pushing the limits of slenderness (>10) and (8) since the number of some supertall building groups (e.g. steel towers) was not sufficient, establishing a scientific relationship between aspect ratio and related design criteria was not possible.Originality/valueTo date, there are no studies in the literature that provide a comprehensive understanding of the interrelationships between the slenderness ratio and the main design criteria in supertall towers (=300 m). This important issue was explored using detailed data collected from 75 cases.
The study of students' discussion process in a teacher-developed problem-solving learning design: Transcript-based lesson analysis of a social studies lesson
Authors: Nozaki Shiho 
Published: October 2022

PurposeThis study aims to clarify the process of students' discussion in a problem-solving lesson developed autonomously by a teacher. It is expected to contribute to the accumulating and theorizing of teachers' professional development in school-based curriculum studies.Design/methodology/approachA transcript-based lesson analysis (TBLA) was conducted using quantitative and qualitative methods. The data were from a third-year junior high school social studies lesson in which whole-class discussions took place. The frequency of the appearance of keywords in the utterances was represented in the form of cumulative relative frequency graphs, and a diagram of the students' discussion process was drawn based on them.FindingsThe diagram confirmed the frequent occurrence, disappearance and transmission of keywords in the process of the lesson. The interpretation of the lesson transcript revealed that the students listened carefully to others' utterances, shared important keywords and examined the ideas critically. They were supported in identifying the connections between their interests by the teacher. The students' interactions and teacher's interventions were facilitated by the integrated curriculum design of teaching and learning.Originality/valueThis study verifies that students' collaborative and critical learning takes place in a lesson developed by a teacher based on the practiced curriculum. The findings of this study suggest that lesson study (LS) focusing on the words in utterances provide clues for evaluating the practiced curriculum and reveal the teacher's professional development.
Granular inflation spillovers
Authors: Esquierro Leon , Da Silva Sergio 
Published: October 2022

PurposeThe authors test the granularity hypothesis to international inflation spillovers using annual exports and inflation data for 138 countries from 1991 to 2020. This study aims to discuss the aforementioned objective.Design/methodology/approachFirst, the authors quantify the power law for the right tail of the export volumes distribution and discuss its implications. Then, the authors compute the granular residual, a measure of shocks to the largest countries.FindingsThe authors find export volumes across countries are not Gaussian-distributed but follow a power law. This finding means the largest countries disproportionately impact world inflation. In addition, the authors find that countries with higher relative weight in international trade determine a portion of international spillovers greater than their trade share. Moreover, eight big grains are responsible for the bulk of inflation spillovers.Practical implicationsThe policy implication is that other countries' central banks should closely monitor the eight big grains when conducting their domestic monetary policy.Originality/valueThis is the first study spotting the problem of granular inflation spillovers.
The impact of COVID-19 pandemic on the volatility of conventional and Islamic stock indexes: a comparative study on ASEAN and GCC countries
Authors: Sundarasen Sheela , Kamaludin Kamilah , Ibrahim Izani 
Published: October 2022

Purpose
The purpose of the study is to adopt Morlet’s wavelet method to examine the differences in the level of volatility (i.e. riskiness) between the conventional and Shari’ah indexes during the COVID-19 pandemic (February 4 to June 19, 2020) on selected Association of South East Asian Nation (ASEAN) and Gulf Cooperation Council (GCC) countries. As a comparison, the equivalent time period of relative tranquillity is used; February 4 to June 19, 2019.

Design/methodology/approach
Morlet’s wavelet method is used in analyzing the volatility levels for both the conventional and Shari’ah indexes before and during the COVID-19 pandemic for the selected ASEAN and GCC countries.

Findings
This study has several findings; first, the markets in the ASEAN region appear to be more volatile during the pandemic than in the GCC region. Second, most of the Shari’ah indexes were more volatile during the COVID-19 pandemic than their conventional counterparts. Nevertheless, the GCC index pairs appear to show more similarities between both the Shari’ah and conventional index.

Practical implications
The findings from this study indicate that investors, government, regulators and all other stakeholders should stay vigilant during a pandemic or health threat period as it has become a pertinent source of volatility spillovers. As such, investors should devise optimal asset allocation strategies, portfolio diversification and portfolio rebalancing measures, taking into consideration not only financial adversity but also public health gravity as a potential source of turbulent markets.

Originality/value
This study uses the wavelet method to examine the volatility level of both the Shari’ah and conventional indexes during the COVID-19 pandemic and its equivalent time frame in 2019. It has further added to the Islamic literature by comparing the volatility between selected ASEAN and GCC countries. The wavelet method is most appropriate for short-duration studies as it captures both the time and frequency domains of the time-series behavior.
Corporate social responsibility (CSR) and sustainability – a scientometric analysis of the interrelationship
Authors: Bhargava Akshay , Ligade Pallavi 
Published: October 2022

Purpose
Traditional economic systems have had serious detrimental effects on environmental development and unequal wealth distribution, culminating in ecological disasters and demographic imbalances. A study of the interrelationships between the principles of sustainability and corporate social responsibility (CSR) would be advantageous, given the prevalence of unequal opportunities and access to resources in a global economy (CSR). To address global and diverse challenges, fieldwork and their relationships must be examined. In this context, the current study aims to investigate the linkages between the concepts of CSR and sustainability to better understand current scientific advances and future research prospects.

Design/methodology/approach
The bibliometric analysis technique is applied in the implementation of this study project. To find the existing links, a total of 3,079 publications on the themes of sustainability and CSR were analysed. The research works from Scopus were chosen because they have a large number of papers, authors and journals while meeting the peer-review standard for scientifictuality, and they have a high number of papers, authors and journals.

Findings
As a result, interest in this field of study has grown significantly, with Garca-Sánchez being the most productive author, Business, Management and Accounting is the most researched topic, and sustainability Switzerland being the most productive publication. The USA has the most articles and citations, whereas Salamanca University is the most prolific producer. Future research should concentrate on the social dimension and its implications for the Circular Economy. Finally, a research line is presented that integrates recommendations from the 2030 Agenda for Sustainable Development and its 17 Sustainable Development Goals.

Originality/value
The paper does fit in bringing the relevant and novel collection of data.
Customer acceptance of online delivery platform during the COVID-19 pandemic: the case of Brunei Darussalam
Authors: Almunawar Mohammad Nabil , Anshari Muhammad 
Published: October 2022

Purpose
As a result of the COVID-19 pandemic, numerous businesses have migrated to an online delivery platform (ODP) to survive and reconnect with their customers. This study aims to focus on how the public perceives ODP. It examined the acceptance of digital platforms for delivering daily necessities, especially food, in Brunei Darussalam during the COVID-19 pandemic.

Design/methodology/approach
The online survey collected 350 valid samples, and the online questions were distributed using a snowball sampling method, with the questionnaire’s softcopy prepared in Qualtrics and sent via email and social media as hyperlinks. In 2021, we sent out the questionnaire link via email, WhatsApp and Facebook to people and organizations for about six months.

Findings
According to the findings of the study, product quality is a critical factor that consumers consider while making online purchases of different products. The COVID-19 condition positively affects customer acceptance, performance, effort and product quality. This research indicates that service quality, online habits and trust do not influence customer acceptance of an ODP.

Research limitations/implications
The study contributes to the body of knowledge regarding factors influencing the acceptance of ODPs. The factors that influence people’s acceptance of ODPs, especially during the COVID-19 pandemic, are assessed as to whether the COVID-19 pandemic influences people’s acceptance and the identified factor that has the strongest influence on the acceptance of digital delivery platforms.

Practical implications
The study contributes to the growing body of knowledge about how customer behaviors triggered by the COVID-19 condition influence customer acceptance of ODP and how behavior influences customer acceptance of ODP.

Originality/value
This paper is useful to academics, practitioners and policymakers in public administration and policy management. The research provides some insights into massive adoption of ODPs that affects the disruption of conventional business practices.
Transdisciplinary multistage system modeling: migrant entrepreneurship in the digital economy
Authors: Satalkina Liliya , Zenk Lukas , Steiner Gerald 
Published: October 2022

PurposeThe dynamics of modern life lead to societal changes that affect innovation systems. Entrepreneurship is an important driver for fostering adaptive capacities of innovation systems in such uncertain and complex environments. This study aims to gain a detailed understanding of how (innovative) entrepreneurship can promote innovation systems, leading to more sustainable societies. A particular focus is placed on migrant entrepreneurship in the digital economy, as a concrete implication of innovative entrepreneurship, and its role within the Austrian innovation system.Design/methodology/approachIn order to develop a shared system understanding from a scientific and practical perspective, transdisciplinary multistage system modeling was applied. The transdisciplinary discourse involved 14 experts, and several system models were iteratively co-created during the course of the research.FindingsThe main result demonstrates the interrelationship between the innovation system and migrant entrepreneurship in the digital economy, which includes six core reinforcing loops: (1) the mindsets of entrepreneurs, (2) the role of international collaboration, (3) the role of entrepreneurial education, the financial sphere in regard to (4) government and (5) private funding, as well as (6) the impact of formal procedures.Originality/valueThe authors present and discuss the relational dynamics of this complex phenomenon as well as the applied transdisciplinary approach, with the aim of identifying a potential way to improve the sustainable impact of (migrant) entrepreneurship considering the case of the Austrian innovation system.