bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel and efficient CD22 CAR-T therapy induced a robust antitumor effect in relapsed/refractory leukemia patients when combined with CD19 CAR-T treatment as a sequential therapy
Authors: Zhang Yu , Li Saisai , Wang Ying , Lu Yang , Xu Yingxi , Rao Qing , Wang Huijun , Xing Haiyan , Tian Zheng , Tang Kejing , Lv Lulu , Wang Min , Wang Jianxiang 
Published: March 2022

AbstractBackgroundCD19 chimeric antigen receptor (CAR) therapy has achieved impressive success in relapsed or refractory (R/R) B-cell malignancies, but relapse due to antigen escape is increasingly appearing reported. As the expression profile of CD22 is similar to that of CD19, CD22 has become a candidate target when CD19 CAR-T therapy fails.MethodsA novel CD22 CAR incorporating scFv derived from an HIB22 hybridoma which bound the first and second Ig-like extracellular domains of CD22 antigen was constructed. Preclinical investigation of the CD22 CAR-T therapy against B-cell malignancies was evaluated by coculturing CD22 CAR-T cells with tumor cell lines or primary blasts from patients in vitro and using a xenograft mouse model in vivo. Further clinical study of CD22/CD19 CAR-T sequential therapy was conducted in 4 R/R adult B-cell acute lymphoblastic leukemia (B-ALL) patients.ResultsThe novel CD22 CAR-T treatment had specific cytotoxicity to CD22 + target cells, and the survival time of mice in the CD22 CAR-T treatment group was significantly prolonged. Furthermore, it’s validated that sequential CD22/CD19 CAR-T therapy was significantly superior than single CD19 or CD22 CAR-T treatment in a relapse xenograft model. All 4 patients achieved complete remission (CR) with negative minimal residual disease (MRD), including 3 patients who had received prior CD19-related immunotherapy. The proliferation of CD19 and CD22 CAR-T cells was observed respectively in vivo, and 3 of the 4 patients experienced cytokine release syndrome (CRS); 2 of these patients had grade 1 CRS and 1 had grade 3 CRS. Long term follow-up showed that 3 of the 4 (75%) patients had sustained CR for up to 1 year. Analysis of antigen expression in the relapsed patients demonstrated that loss or diminution of CD19 and CD22 expression might cause antigen escape from CAR-T surveillance.ConclusionsIn summary, the novel CD22 CAR-T therapy was validated with antitumor effects both in vitro and in vivo. Furthermore, our study demonstrated the safety and robust efficacy of sequential CD22/CD19 CAR-T therapy in xenograft models and clinical trials, especially as the salvage treatment for R/R B-ALL patients with antigen loss or in whom anti-CD19 related immunotherapy failure failed.Trial registration:Chinese Clinical Trial Registry (ChiCTR): ChiCTR1900025419, Supplementarily registered 26 August, 2019.
Trial-to-trial modulation of task-order switch costs survive long inter-trial intervals
Authors: Strobach Tilo , Wendt Mike 
Published: March 2022

Abstract
Background
Dual-tasking procedures often involve the successive presentation of two different stimuli, requiring participants to execute two tasks in a particular order. Performance in both tasks suffers if the order of the tasks is reversed (i.e., switched) compared to the directly preceding trial. This task-order switch cost is reduced, however, if the preceding trial itself involved a task-order switch compared to a task-order repetition (Strobach in Acta Psychol 217:103328, 2021). Theoretical accounts range from assumptions of top-down implementation of a task-order control set, or passive persistence thereof, to priming based on episodic binding of tasks and temporal positions. Here, we tested these accounts by investigating whether the sequential modulation decays as a function of the inter-trial interval.

Methods and results
Task-order switch costs were reliably reduced after a task-order switch (compared to after a task-order repetition) and this reduction did not decrease over inter-trial intervals ranging from 350 ms to 1,400 ms. Also replicating previous findings, for reaction times the reduction was driven by selective slowing in task-order repeat trials, suggesting increased response caution.

Conclusions
Our results are consistent with preparatory processes of task-order control or with episodic integration of task-order information but argue against accounts assuming short-lived, decaying task-order sets.
Restoration of Tanjore paintings using segmentation and in-painting techniques
Authors: Poornapushpakala S. , Barani S. , Subramoniam M. , Vijayashree T. 
Published: March 2022

Abstract
India has its unique and rich cultural heritage. One such uniqueness in India is ancient paintings. Especially in South India, Tanjore paintings are very popular. These paintings are made during 1010 AD with vibrant colours, gold, silver and precious stones. These paintings are the memorabilia of the great Chola kingdom. These paintings can be seen in great Brahadeeshwara Temple walls till now. Damages to these paintings happen due to varying environmental conditions and rituals followed throughout the year. Hence, preserving these heritages could be an additional source in National Cultural Museum and cultural libraries. This paper focuses towards the restoration of such ancient painting images that can be digitized and archived for the future use of aesthete. The painting images are preprocessed using Weiner filter for removing the background noises since its PSNR value is higher than Gaussian and Median filters. The preprocessed image is then applied to restoration algorithm. Two types of restoration algorithm is attempted, image segmentation and in-painting algorithm. The degraded image was restored efficiently with in-painting algorithm than segmentation algorithm. Further research can be focused towards automatic adaptive selection of patch based on the nature of images. From the results it is observed that with in-painting algorithm the image restoration is better than the segmentation algorithm for degraded painting images.
Exercise interveNtion outdoor proJect in the cOmmunitY—ENJOY program for independence in dementia: a feasibility pilot randomised controlled trial study protocol
Authors: Levinger Pazit , Goh Anita M. Y. , Dunn Jeremy , Katite Josephine , Paudel Ritu , Onofrio Adrian , Batchelor Frances , Panisset Maya G. , Hill Keith D. 
Published: March 2022

Abstract
Background
While the underlying neuropathology of dementia is not curable, interventions and treatment, such as physical activity, can offer physical and functional gains leading to better mobility, independence and quality of life. The Seniors Exercise Park program is an evidence-based physical and social activity program using an innovative design in outdoor exercise equipment specifically designed for older people. This unique program has never been tested with older people living with dementia. This study will evaluate the feasibility of delivering the Seniors Exercise Park program for people living with mild to moderate dementia in residential aged-care. This study will identify the optimal physical activity program, evaluate the safety of equipment usage and determine optimal supervision needs. The potential physical, social, quality of life and cognitive benefits of participation in the Seniors Exercise Park program will also be examined.

Methods
This is a feasibility pilot randomised controlled design with pre-post evaluation. Adults aged ≥ 60 years who have symptoms of dementia and/or who have been diagnosed with dementia will be recruited from an aged-care facility in Melbourne. Participants allocated to the intervention group will undergo a 12-week structured supervised physical activity program using the outdoor Seniors Exercise Park equipment followed by a 12-week maintenance phase (unstructured physical activity). Participants will be assessed at baseline, 3 and 6 months. Participants allocated to the control group will attend activities provided by the aged-care facility. A sample of 12 participants per group is the targeted sample size. Feasibility will be evaluated in terms of recruitment rate, retention, attendance, overall adherence, dropout rate, adverse events, modifications to the exercise program delivery and supervision needs. A comprehensive suite of cognitive and health-related questionnaires and physical function measures will also be collected.

Discussion
The ENJOY program for independence in dementia will determine the suitability of the Seniors Exercise Park program for people diagnosed with mild to moderate dementia. Outcomes could inform future design of dementia-friendly built environments to increase physical activity participation for residential aged-care facilities.

Trial registration
This trial is registered with the Australian New Zealand Clinical Trials Registry—Registry Number ACTRN12620000733976. Registered on the 13th of July 2020.
Improving chronic disease self-management in high-risk patient populations
Authors: Patel Ashruta 
Published: March 2022

AbstractChronic diseases can be prevented, controlled, or modified through healthy behaviors, and these changes have the potential to produce major transformations in the current health care system. Innovative approaches to execute effective transitional care to reduce health care costs, decrease re-hospitalization, and improve quality care. Studies have indicated the use of health coaching as a potential method for improving self-management, with the aim of improving compliance with health behaviors and sustainable lifestyle changes. Post-discharge follow-up using appropriate transitional models can influence the likelihood of patient’s management of care at home and can help determine what re-admission prevention programs can be effective. Gathering information from patient-centered, evidence-based efforts conducted for better self-management of chronic disease and effective transition from hospital to home. Hospitals should consider using a quality improvement infrastructure that incorporates patient perceptions, mental and physical status’, transition coordination, and self-management sessions that are patient-centered for implementation to ultimately reduce the risk of re-hospitalization in patients who suffer from chronic conditions.
Therapeutic effect of intra-articular injected 3′-sialyllactose on a minipig model of rheumatoid arthritis induced by collagen
Authors: Kim Young June , Lee Ju-young , Yang Mi-jin , Cho Hyun Jin , Kim Min-young , Kim Lila , Hwang Jeong Ho 
Published: March 2022

Abstract
Background
Rheumatoid arthritis (RA) is a chronic inflammatory disease of joint, but there is no known cure.
3′-sialyllactose (3′-SL) is an oligosaccharide that is abundant in breast milk of mammals, and has anti-inflammatory properties. However, the efficacy of 3′-SL on RA remains unclear. The objective of the present study was to evaluate the therapeutic effect of 3′-SL after it was directly injected into the knee joint cavity of a RA minipig model.

Results
Minipig RA model was induced by intra-articular injection of bovine type II collagen emulsified with complete or incomplete Freund’s adjuvant into left knee joint. In clinical assessment, lameness and swelling of the hindlimb and increased knee joint width were observed in all animals. After the onset of arthritis, 3′-SL (0, 2, 10, and 50 mg/kg) was directly administered to the left knee joint cavity once a week for 4 weeks. Compared to the vehicle control group, no significant difference in macroscopic observation of the synovial pathology or the expression of inflammation-related genes (IL-1β, TNF-α, and COX2) in the synovial membrane of the knee joint was found. In microscopic observation, cell cloning of the articular cartilage was significantly reduced in proportion to the concentration of 3′-SL administered.

Conclusions
Our results suggest that intra-articular injected 3′-SL had a therapeutic effect on collagen-induced arthritis at the cellular level with potential as a medication for RA.
Association of OPRM1 with addiction: a review on drug, alcohol and smoking addiction in worldwide population
Authors: Hriatpuii Vanlal , Sema Hoikhe Priscilla , Vankhuma Chenkual , Iyer Mahalaxmi , Subramaniam Mohana Devi , Rao Krothapalli R. S. Sambasiva , Vellingiri Balachandar , Kumar Nachimuthu Senthil 
Published: March 2022

Abstract
Background
Drugs are chemicals which can disrupt the nerve cell functions of the brain. The present study aims to investigate the addiction related gene (OPRM1) in three types of addiction—drugs, alcohol and smoking. Pathway for the addiction was ascertained through KEGG database, and the hotspot mutations for various populations were identified from Gnomad-exomes database. In silico analyses like SIFT, Polyphen, Hope, I-mutant and mutation taster were performed to understand the amino acid substitution, protein function, stability and pathogenicity of the variants.

Main body
Addiction-related variants were found in exons 1, 2 and 3, while the exon 4 did not exhibit any addiction related variation. Among all the variants from this gene, rs1799971 (A118G) polymorphism was the most commonly studied variation for addiction in different populations worldwide. Population-wise allele and genotype frequencies, demographic and epidemiological studies have also been performed from different populations, and the possible association of these variants with addiction was evaluated.

Conclusion
Our findings suggest that OPRM1 polymorphism impact as pharmacogenetic predictor of response to naltrexone and can also address the genetic predisposition related to addiction in human beings.
Increased expression of IL-37 correlates with TNF-α levels and disease stage in endometriosis patients
Authors: Ragab Dina , Abbas Ahmed , Salem Ramy 
Published: March 2022

Abstract
Background
IL-37 is an anti-inflammatory cytokine that increases in several inflammatory diseases with the main inducing signal for its production being pro-inflammatory cytokines like TNF-α. We aimed to assess the correlation between peritoneal fluid levels of IL-37 and TNF-α in endometriosis patients and investigate their association with disease stage. Levels of IL-37 and TNF-α were assessed in peritoneal fluid of 50 patients with endometriosis and 23 endometriosis-free females using enzyme-linked immunosorbent assay. We also assessed serum levels of IL-37 using enzyme-linked immunosorbent assay and expression of IL-37 mRNA in peritoneal fluid cells using polymerase chain reaction.

Results
Peritoneal fluid levels of IL-37 and TNF-α were higher in endometriosis patients than in control females. Also, levels were higher in patients with late endometriosis than patients with early endometriosis. In addition, serum levels of IL-37, as well as IL-37 mRNA expression in peritoneal fluid cells, were higher in patients than controls. In endometriosis patients, peritoneal fluid levels of IL-37 positively correlated with levels of TNF-α.

Conclusion
Collectively, our results show increased levels of the anti-inflammatory cytokine IL-37 in endometriosis patients that correlate with levels of the pro-inflammatory cytokine TNF-α, one of the main signals for IL-37 production.
The association between Internet addiction and sleep quality among Sohag University medical students
Authors: Mahmoud Osama Abd Alreheem , Hadad Saber , Sayed Taher Abdelraheem 
Published: March 2022

Abstract
Background
Internet addiction (IA) is a growing issue with multiple impacts on psychological functions, including sleep. Consequently, the present study aimed to assess the correlation between IA as well as the quality of sleep among medical students. A total of 525 students from Sohag University were included in the study. Young Internet Addiction Test (YIAT) was utilized in order to evaluate IA. Demographic information, university-related factors, and Internet usage patterns of the participants were documented. Pittsburgh Sleep Quality Index was utilized so as to evaluate the quality of sleep.

Results
The prevalence of IA was 4.95% for severe addiction and 39.62% for moderate addiction. About 81.62% of subjects who suffer from IA significantly had poor quality of sleep. Young age, male gender, low academic performance, computer presence at home, Internet presence at home, using the Internet for chatting and gaming, and poor sleep quality are strong predictors of Internet addiction.

Conclusions
The prevalence of IA and its impact on the quality of sleep have been found to be substantial among medical students. There is a need for programs to raise awareness towards IA as well as its impact on sleep among university medical students.
Construction and Verification of Risk Predicting Models to Evaluate the Possibility of Venous Thromboembolism After Robot-Assisted Radical Prostatectomy
Authors: Cheng Xu , Zhou Lizhi , Liu Wentao , Li Yijian , Peng Mou , Wang Yinhuai 
Published: March 2022

Abstract
Background
Venous thromboembolism (VTE) is the second leading cause for death of radical prostatectomy. We aimed to establish new nomogram to predict the VTE risk after robot-assisted radical prostatectomy (RARP).

Methods
Patients receiving RARP in our center from November 2015 to June 2021, were enrolled in study. They were randomly divided into training and testing cohorts by 8:2. Univariate and multivariate logistic regression (model A) and stepwise logistic regression (model B) were used to fit two models. The net reclassification improvement (NRI), integrated discrimination improvement (IDI), and receiver operating characteristic (ROC) curve were used to compare predictive abilities of two new models with widely used Caprini risk assessment (CRA) model. Then, two nomograms were constructed and received internal validation.

Results
Totally, 351 patients were included. The area under ROC of model A and model B were 0.967 (95% confidence interval: 0.945–0.990) and 0.978 (95% confidence interval: 0.960–0.996), which also were assayed in the testing cohorts. Both the prediction and classification abilities of the two new models were superior to CRA model (NRI > 0, IDI > 0, p < 0.05). The C-index of Model A and Model B were 0.968 and 0.978, respectively. For clinical usefulness, the two new models offered a net benefit with threshold probability between 0.08 and 1 in decision curve analysis, suggesting the two new models predict VTE events more accurately.

Conclusions
Both two new models have good prediction accuracy and are superior to CRA model. Model A has an advantage of less variable. This easy-to-use model enables rapid clinical decision-making and early intervention in high-risk groups, which ultimately benefit patients.
Materials Nanoarchitectonics from Atom to Living Cell: A Method for Everything
Authors: Ariga Katsuhiko , Fakhrullin Rawil 
Published: March 2022

AbstractPromoted understanding of nanostructures and their functions significantly rely on rapid progress of nanotechnology within a few decades. It would be a fruitful way to consider materials science through regulation of nanostructure regulations on the basis of nanotechnological accomplishments. As a post-nanotechnology concept, a new concept of nanoarchitectonics has recently emerged as a methodology to produce functional materials from nanoscale units. In this review article, we discuss the recent advances of materials nanoarchitectonics and related approaches from atom-scales to living cell sizes. The approaches on multiscale nanoarchitectonics are exemplified and discussed including atom and molecules nanoarchitectonics, supramolecular nanoarchitectonics, and nanoarchitectonics at cell-scale. Next, supracell-level nanoarchitectonics is discussed including living cell organization at designed surfaces, cell sheets and layered nanoarchitectonics, three-dimensional cell nanoarchitectonics, biofilms and bioinks, and new challenges for living cells at liquid interface. All the materials are formed with fundamental units, atoms, molecules, and nanomaterials, and therefore, the nanoarchitectonics approaches from basic units to materials can be hypothetically applied to any kind of material. Therefore, nanoarchitectonics could be a method for everything in materials science as an analogy to a theory of everything in physics.
Lewis Acid-catalyzed Decarboxylative Cyanation of Cyclic Enol Carbonates — Access to Multi-substituted β-Ketonitriles —
Authors: Yokoyama Haruki , Dokai Yoichi , Kimaru Natsuki , Saito Kodai , Yamada Tohru 
Published: March 2022

Abstract
A Lewis acid-catalyzed decarboxylative substitution reaction of cyclic enol carbonates with a cyanation reagent was developed to give β-ketonitriles in good yield. It is proposed that the reaction proceeds through an oxyallyl cation intermediate or its equivalent. Interestingly, cyanation selectively took place at the more hindered site of the intermediate to afford a multi-substituted β-ketonitrile. This regioselectivity was observed by the cyanation of a wide range of substrates and is discussed in terms of the reaction mechanism.
CYP2C19 polymorphism and coronary in-stent restenosis: A systematic review and meta-analysis
Authors: Pintaningrum Yusra , . Vitriyaturrida , Dewi Ivana Purnama , Putra Hendy Bhaskara Perdana , Mappangara Idar , Amir Muzakkir , Yusuf Irawan , Bukhari Agussalim 
Published: March 2022

Background: In-stent restenosis (ISR) remains a major drawback in coronary stenting. The association between the CYP2C19 loss of function (LOF) gene and the prevalence of ISR after coronary stenting remains controversial. Previous studies have produced conflicting results and have been limited by their small population sizes. We conducted this systematic review and meta-analysis to determine the association between the presence of the CYP2C19 LOF gene and the prevalence of ISR. Methods: A systematic online database search was performed until April 2021. The primary outcome was ISR and assessed using OR with 95% CI. Publication bias was assessed using the Newcastle Ottawa Scale. I2 was applied to examine heterogeneities among the studies. Results: A total of 284 patients (four non-randomized controlled trial studies) were included in this study. Two hundred and six patients had wild-type genotypes, while 78 patients had the LOF genotype. Among the 78 patients with the LOF gene, 40 patients had an ISR. Meanwhile, of the 206 patients with a wild-type gene, 69 patients had an ISR. The LOF gene was associated with a higher risk of ISR (OR 95% CI = 2.84 [1.54–5.24], p = 0.0008). A major limitation in our study was the small number of previous studies and small sample sizes. Conclusions: Patients with LOF genes, regardless of the allele variation, treated with clopidogrel, had a higher risk of developing ISR after coronary stenting.