bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting Lung Cancer Survival after Curative Surgery Using Deep Learning of Diffusion MRI
Authors: Moon Jung Won , Yang Ehwa , Kim Jae-Hun , Kwon O Jung , Park Minsu , Yi Chin A 
Published: October 2022

Abstract
The survival of lung cancer patients is expected differently according to the stage at diagnosis. However, each individual patient experiences different survival results even in the same stage group. DWI and ADC are two of widely used prognostic indicators for the prediction of survival in cancer patients. This study aims to develop a deep learning model that predicts the overall survival of non-small cell lung cancer patients using diffusion MRI. The study adapted a VGG-16 network and investigated the model’s performance using different combinations of DWI with/without ADC images. The survival model using deep learning of both DWI and ADC accurately predict the possibility of survival in five years after surgical treatment of NSCLC (up to 92%). The accuracy of results produced by the deep learning model can be enhanced by inputting precedented, proven functional parameters of ADC including the original images of DWI in survival prediction.
Recovery of physical function, muscle mass and quality of life in patients undergoing allogeneic hematopoietic stem cell transplantation
Authors: Takekiyo Takahiro , Utsunomiya Atae , Nara Souichiro , Mori Nozomi , Nakashima Norihisa , Okamura Toshiyuki , Tokunaga Masahito , Miyazono Takayoshi , Nakano Nobuaki , Ito Yoshikiyo , Dozono Koichiro 
Published: October 2022

Abstract
Purpose
This study aimed to investigate the recovery of physical function, muscle mass, and quality of life (QOL) in allogeneic hematopoietic stem cell transplantation (allo-HSCT) patients 1 year after the procedure.
Methods
A total of 71 patients who underwent allo-HSCT at our institution between February 2010 and June 2020, for whom a physical therapy assessment could be performed before allo-HSCT, at discharge, and 1 year after allo-HSCT, were included. Exercise therapy during hospitalization was provided individually by a physical therapist, and exercise was self-administered after discharge.
Results
One year after allo-HSCT, handgrip strength and results of the 6-minute walk test (6 MWT) recovered to pre-HSCT levels. Muscle mass 1 year after allo-HSCT showed slight improvement after discharge from the hospital but did not reach the pre-HSCT level. All subscales of QOL, 1 year after allo-HSCT, recovered to pre-HSCT levels, but only two of the eight subscales recovered to the national norm of 50. Multivariate analysis revealed factors associated with the recovery of physical function and QOL, including improved hemoglobin levels, albumin levels, and adherence to exercise therapy. In contrast, factors that negatively affected recovery were steroid administration and pre-HSCT intensity conditioning.
Conclusion
The results suggest that continued exercise therapy may contribute to the recovery of muscle strength, endurance, and QOL 1 year after allo-HSCT.
Procedure for the implementation of VR/AR learning scenarios for method training - Presentation of the Assisted Reality Implementation Model (ARIM).
Authors: Karcher Amelie , Arnold Dominik , Prinz Christopher , Kuhlenkötter Bernd 
Published: October 2022

Abstract
Virtual - and Augmented Reality (VR/AR) based assistance systems represent a growing technology approach for the sustainable communication of quality methods for further education offers. To create virtual learning environments as standardized and efficient as possible, a uniform approach is required. This paper focuses on the development of the Assisted Reality Implementation Model (ARIM) for the selection of Lean Management and Six Sigma quality methods that are suitable to be taught using augmented and virtual reality learning environments. Furthermore, the ARIM enables meaningful guidance for the implementation of the virtual learning environments, as well as subsequent validation. The model will be applied and evaluated within the research project WILLEN.
New observations on the Antarctic Asteromphalus darwinii/ hookeri diatom species-complex (Asterolampraceae)
Authors: Hallegraeff Gustaaf , LeBlanc Karine 
Published: October 2022

Abstract
Antarctic diatom populations of Asteromphalus hookeri and related species such as A. hyalinus and A. parvulus exhibit a highly variable number of hyaline rays ranging from 3 broad + 1 narrow (3 + 1) in the smallest valves, with 4 + 1 (27%) and 5 + 1 rays (35%) most common, and 6 + 1, 7 + 1, and rarely 8 + 1 rays only in larger cells. During December 1959 to April 1960 in the southern sector of the Atlantic Ocean, 6% of valves occurred as “double forms” with epitheca and hypotheca of the same cell exhibiting 4 + 1/3 + 1, 5 + 1/4 + 1, 6 + 1/5 + 1 and 7 + 1/6 + 1 ray combinations. Smaller cells (3 + 1, 4 + 1) always exhibited jagged separation lines in the central area, but larger cells (7 + 1, 8 + 1) had mostly smooth lines, and either jagged or smooth separation lines occurred in intermediate 5 + 1 and 6 + 1 forms, respectively. Epitheca and hypotheca of one and the same cell always exhibited jagged or smooth separation lines, but never mixtures. Observations of silica deposition during October to November 2011 around the Kerguelen Island plateau using the PDMPO fluorescent marker suggest that Asteromphalus separation lines play a key role in silica cell wall development. We discuss implications for taxonomy and our understanding of ecophysiology of what we designate as two highly variable and often confused and overlapping diatom taxa, A.darwiniii (jagged separation lines; synonyms A. beaumontii, A. hyalinus, A. leboimei, A. parvulus, A. rossii) and A. hookeri (smooth separation lines; synonym A. antarcticus, A.buchii, ?cuvierii, ?humboldtii).
Home range and habitat selection of wild boar (Sus scrofa) in rural landscape
Authors: Ikeda Takashi , Higashide Daishi , Suzuki Takaaki , Asano Makoto 
Published: October 2022

Abstract
Wild boars are cause crop damage and are vectors causing infectious diseases in humans and livestock, thus wild boar population management is essential to control the damage. Because information on mammal home range and habitat selection is important for the management of target species, accurate spatial data would provide valuable information to establish population management scheme and infectious diseases countermeasure. In this study, we aimed to understand the home range of wild boars and their habitat selection during the daytime and nighttime. We captured seven wild boars in in central Gifu Prefecture, Japan, and monitored each individual from October 2020 to June 2022. We calculated the 95% and 50% home range of each individual for the entire monitoring period and for short-term period. Additionally, we investigated environmental characteristics at GPS locations during daytime and nighttime. In this study, the home range sizes from the minimum convex polygon method ranged from 0.32 to 28.51 km2, and the maximum distance between the location points was 7.6 km. Moreover, wild boars avoided anthropogenic environments during the daytime and selectively used cropland category during the nighttime. Therefore, wild boars had narrow home ranges depending on the anthropogenic environments. Consequently, wildlife managers could reduce agricultural damage caused by wild boars by managing bamboo forests and abandoned cultivated lands in the rural landscape. For infectious diseases, moreover, they should implement countermeasures against wild boars that are within 8 km of pig farms.
Traditional and New Lifestyle Interventions to Prevent Breast Cancer Recurrence (TANICA): A qualitative study
Authors: Aflague Tanisha F. , Esq Monica K. , Hammond Kristi , Reyes Bernice Delos , Kaholokula Joseph Keawe 
Published: October 2022

Abstract
Purpose: Breast cancer is the second cause of death from cancer in Guam and Hawai‘i and disproportionately impacts Native Hawaiian, CHamoru, and Filipino women. Although a few culturally informed interventions addressing breast cancer survivorship exist, none have been developed or tested for Native Hawaiian, CHamoru, and Filipino women. To address this, the TANICA study began with key informant interviews in 2021.
Methods: Purposive sampling and grounded theory approaches were used to conduct semi-structured interviews with individuals experienced in providing healthcare or implementing community programs and/or research with ethnic groups of interest in Guam and Hawai‘i. A literature review and expert consultation identified intervention components, engagement strategies, and settings. Interview questions aimed to understand the relevance of evidence-based interventions and explored socio-cultural factors. Participants completed demographics and cultural affiliation surveys. Interviews were independently analyzed by trained researchers. Themes were mutually agreed upon by reviewers and key themes were identified based on frequencies.
Results: Nineteen interviews were conducted in Hawai‘i (n=9) and Guam (n=10). Interviews confirmed the relevance of most of the previously identified evidence-based intervention components for Native Hawaiian, CHamoru, and Filipino breast cancer survivors. Ideas around culturally responsive intervention components and strategies emerged that were shared across and unique to each ethnic group and site.
Conclusion: Evidence-based intervention components appear relevant, yet cultural and place-based strategies are needed for Native Hawaiian, CHamoru, and Filipino women in Guam and Hawai‘i. Future research should triangulate these findings with the lived experiences of Native Hawaiian, CHamoru, and Filipino breast cancer survivors to develop culturally informed interventions.
Doublecortin-expressing neurons in human cerebral cortex layer II and amygdala from infancy to 100 year-old
Authors: Li Ya-Nan , Hu Dan-Dan , Cai Xiao-Lu , Wang Yan , Yang Chen , Jiang Juan , Zhang Qi-Lei , Tu Tian , Wang Xiao-Sheng , Wang Hui , Tu Ewen , Wang Xiao-Ping , Pan Aihua , Yan Xiao-Xin , Wan Lily 
Published: October 2022

Abstract
A cohort of morphologically heterogenous doublecortin immunoreactive (DCX+) “immature neurons” has been identified in the cerebral cortex largely around layer II and the amygdala largely in the paralaminar nucleus (PLN) among various mammals. To gain a wide spatiotemporal view on these neurons in humans, we examined layer II and amygdalar DCX + neurons in the brains of infants to 100 year-old individuals. Layer II DCX + neurons occurred throughout the cerebrum in the infants/toddlers, mainly in the temporal lobe in the adolescents and adults, and only in the temporal cortex surrounding the amygdala in the elderly. Amygdalar DCX + neurons occurred in all age groups, localized primarily to the PLN, and reduced in number with age. The small-sized DCX + neurons were unipolar or bipolar, and formed migratory chains extending tangentially, obliquely and inwardly in layers I-III in the cortex, and from the PLN to other nuclei in the amygdala. Morphologically mature-looking neurons were lightly labeled and co-expressed the neuron-specific nuclear antigen, glutamic acid decarboxylase and nicotinamide adenine dinucleotide phosphate-diaphorase. Taken together, we consider that the layer II and amygdalar DCX + neurons form a slowly developing interneuron system that supports inhibitory network plasticity in the cerebrum in an age/region-dependent manner.
Effects of bacterial inoculants on microbial community, mycotoxin contamination, and aerobic stability of corn silage infected in field by toxigenic fungi during aerobic exposure
Authors: Wang Wenbo , Wang Wenkang , Shao Tao , Ma Pengfei , Li Junfeng , Zhao Jie , Gallo Antonio , Yuan Xianjun 
Published: October 2022

Abstract
This study was aimed to evaluate the effects of inoculants on the microbial community and mycotoxins contamination during aerobic exposure of corn silage. Whole-crop corn infected with or without mycotoxigenic fungi were ensiled with Lactobacillus buchneri (LB, 1.0×106 cfu g− 1 fresh weight (FW)), Lactobacillus plantarum (LP, 1.0×106 cfu g− 1 FW), or LBLP at 1.0× 106 cfu g− 1 FW each. The higher concentration of AcA (P < 0.05) in LB and LBLP silages than C and LP of NFI silages. Fungal infection resulted in a larger increase of zearalenone (ZEN, P = 0.01), fumonisin B1 (FUB1, P = 0.02), and fumonisin B2 (FUB2, P = 0.02). The RA of Issatchenkia in NFI was higher (P < 0.001) than FI silages, whereas the RA of Kazachstania (P < 0.001), Zygosaccharomyces (P = 0.047), and Candida (P = 0.025) in NFI were lower than these of FI silages. The aerobic stability was improved by the application of LB and LBLP as compared with C of NFI silages. The LB and LBLP had the potential to improve aerobic stability and alleviate mycotoxins contamination of non-fungal infected corn silages, but did not mitigate the negative effect of fungal infection in corn silages.
In Silico Track to Reveal a Translational Potential of Porphyrin-c60 Nanoparticles in the Ischemic Stroke Related Preclinical Studies
Authors: Fursov Valentin , Namestnikova Daria , Kuznetsov Dmitriy A. 
Published: October 2022

Abstract
The In Silico study on neuropharmacokinetiks of some novel porphyrin-fullerene based 25Mg2+ - nanocarriers was performed with an aim to optimize the preclinical research path required for both prevention and correction of the brain ischemic stroke related metabolic disorders such us ATP deplete and its direct consequences. Thus, the local brain tissue hypoxia scenario are in a focus of this novel analytical approach suitable for prediction of some parameters of the 25Mg – magnetic isotope effect promoted antihypoxic activities as long as they relates upon delivery, distribution and intralization of the low toxic/amphiphilic Mg2+ – releasing nanoparticles of PMC16 type. This is the first report ever on mathematical model applied to predict and to prove a mere phenomenon of the “cellular pump” keeping the constant traffic of PMC16 particles towards a brain hypoxia area even when/if lowest concentration of pharmacophore were the case. For experimental verifications of the In Silico platform proposed, a combination of (a) the rat brain occlusion – promoted ischemic stroke model and (b) the capillary zone electrophoretic (CZE) quantification of PMC16-RX nanoparticles in cytosol fractions isolated from intact / penumbra / stroke brain areas, has been employed.
Induction of promyelocytic leukemia zinc finger protein by miR-200c-3p restores sensitivity to anti-androgen therapy in androgen-refractory prostate cancer and inhibits the cancer progression via down-regulation of integrin α3β4
Authors: Dahal Sadan , Chaudhary Prakash , Kim Jung-Ae 
Published: October 2022

Abstract
Purpose
Androgen-refractory prostate cancer (ARPC) is one of the aggressive human cancers with metastatic capacity and resistance to androgen deprivation therapy (ADT). The present study investigated the genes responsible for ARPC progression and ADT resistance, and their regulatory mechanisms.
Methods
Transcriptome analysis, co-immunoprecipitation, confocal microscopy, and FACS analysis were performed to determine differentially-expressed genes, integrin α3β4 heterodimer, and cancer stem cell (CSC) population. miRNA array, 3′-UTR reporter assay, ChIP assay, qPCR, and immunoblotting were used to determine differentially-expressed microRNAs, their binding to integrin transcripts, and gene expressions. A xenograft tumor model was used to assess tumor growth and metastasis.
Results
Metastatic ARPC cell lines (PC-3 and DU145) exhibiting significant downregulation of ZBTB16 and AR showed significantly upregulated ITGA3 and ITGB4. Silencing either one of the integrin α3β4 heterodimer significantly suppressed ARPC survival and CSC population. miRNA array and 3′-UTR reporter assay revealed that miR-200c-3p, the most strongly downregulated miRNA in ARPCs, directly bound to 3′-UTR of ITGA3 and ITGB4 to inhibit the gene expression. Concurrently, miR-200c-3p also increased PLZF expression, which, in turn, inhibited integrin α3β4 expression. Combination treatment with miR-200c-3p mimic and AR inhibitor enzalutamide showed synergistic inhibitory effects on ARPC cell survival in vitro and tumour growth and metastasis of ARPC xenografts in vivo, and the combination effect was greater than the mimic alone.
Conclusion
This study demonstrated that miR-200c-3p treatment of ARPC is a promising therapeutic approach to restore the sensitivity to anti-androgen therapy and inhibit tumor growth and metastasis.
Predicting the dietary fiber content of fresh-cut bamboo shoots using a visible and near-infrared hyperspectral technique
Authors: Xu Xiao-Yan , Xie Wei-Guang , Xiang Cheng , You Qian , Tian Xing-Guo 
Published: October 2022

Abstract
The dietary fiber content in fresh-cut bamboo shoots is considered crucial for the quality of processed bamboo shoots products. This study aimed to explore the potential of applying two different hyperspectral techniques, namely visible near infrared (Vis-NIR) spectroscopy and near infrared (NIR) in the quick and non-destructive prediction of the dietary fiber content of fresh-cut bamboo shoots. The Vis-NIR and NIR hyperspectral data were collected to establish partial least square regression (PLSR) and principal component regression (PCR) calibration model for the average spectrum of fresh-cut bamboo shoots and their corresponding dietary fiber content. Subsequently, data fusion analysis, various pre-processing methods, and principal component analysis (PCA) were used to optimize the model. The results indicated that superior models were obtained based on low-level fusion data when compared with the corresponding methods based on single spectral data. The optimal SNV-PCA-PLSR model achieved a good performance with coefficient of determination of prediction (R2p) of 0.902, and root mean square errors of prediction (RMSEP) of 0.135. Therefore, hyperspectral technique combined with data fusion analysis can be a promising approach for non-invasive quality supervision of bamboo shoots products in varied processing states.
Influence of Bovine Arch Anatomy on Surgical Outcomes of Coarctation of the Aorta
Authors: Miwa Koji , Iwai Shigemitsu , Tsumura Sanae , Kanaya Tomomistu , Tominaga Yuji , Kawai Shota , Teguri Yuta 
Published: October 2022

Abstract
The study aimed to evaluate the outcome of coarctation of the aorta (CoA) repair with special interest in bovine arch anatomy. Fifty-six patients who underwent CoA repair between 2010 and 2021 were included in this retrospective study. Of these, 11 patients had bovine arch anatomy. Surgical outcomes were reviewed. Computed tomography was used to analyze aortic arch geometry routinely prior to surgery. The gap between anastomotic sites was calculated at the linear region of the lesser curvature of the aortic arch between the distal ascending aorta and the proximal descending aorta. CoA repair was performed at median age of 39 days (body weight, 3.3 kg). Thirty-two patients underwent extended direct anastomosis, 22 patients underwent direct anastomosis, and 2 patients underwent lesser curvature patch augmentation. The median follow-up period was 47 months. There were no early deaths. In patients who underwent direct and extended direct anastomosis, 6 recoarctation and 1 left pulmonary venous obstruction events occurred. Moreover, freedom from adverse events was 81 % in normal arch and 50 % in bovine arch cases at 10 years (P = 0.04). Two patients with a bovine arch anatomy who underwent lesser curvature patch augmentation had good outcomes. The distal arch was narrower and longer, and the gap between anastomotic sites was longer in patients with a bovine arch anatomy than with a normal arch (P< 0.01). In CoA with a bovine arch anatomy, the gap between anastomotic sites was long. This adversely influenced the outcomes of direct anastomosis.
DDX39B facilitates the malignant progression of hepatocellular carcinoma via activation of SREBP1- mediated de novo lipid synthesis
Authors: feng tianyu , Li Siqi , Zhao Gang , Li Qin , Yuan Hang , Zhang Jie , Gu Rui , Ou Deqiong , Guo Yafei , Kou Qiming , Wang Qijing , Li Kai , Lin Ping 
Published: October 2022

Abstract
Purpose
The detailed molecular mechanisms of aberrant lipid metabolism in HCC remains unclear. Herein, we focused on the potential role of DDX39B in aberrant lipogenesis and malignant development in HCC.
Methods
DDX39B expression in HCC and para-cancer tissues was measured by immunohistochemistry. CCK-8, clone formation and transwell assays were utilized to detect HCC cells proliferation, migration and invasion in virto. Oil red O, nile red staining, triglyceride and cholesterol detections were used to measure lipogenesis. Co-immunoprecipitation was used to detect interactions between DDX39B and SREBP1. Fractionation and immunofluorescence assays were performed to investigate the impact of DDX39B on SREBP1 nuclear translocation. Luciferase assay was used to explore transcriptional activity of SREBP1. Nude mice subcutaneous and orthotopic xenograft models were performed to verify the contribution of DDX39B/SREBP1 axis in tumor growth, lung metastasis and lipid synthesis in vivo.
Results
DDX39B is upregulated in HCC tissues and predicts worse prognosis. Upregulated DDX39B contributes to the proliferation, metastasis and lipogenesis of HCC cells. Mechanistically, DDX39B directly interacts with SREBP1 and silencing DDX39B impairs the stabilization of the SREBP1 protein through FBXW7-mediated ubiquitination and degradation of SREBP1. Furthermore, DDX39B deficiency decreases the nuclear translocation and activation of SREBP1 and transcription of SREBP1 downstream genes, resulting in reduced lipid accumulation.
Conclusions
Our study reveals a novel mechanism by which DDX39B facilitates the malignant progression of HCC via activation of SREBP1-mediated de novo lipogenesis, implicating DDX39B as both a potential predictor of recurrence and prognosis and a promising therapeutic target.
Effects of intermittent starvation on growth performance, precocity, digestive enzyme activity and body chemical composition of juvenile Chinese mitten crab Eriocheir sinensis
Authors: Li Xiaowu , Zhang Tanglin , Li Wei , Liu Jiashou 
Published: October 2022

Abstract

The effects of intermittent starvation on the growth, precocity, digestive enzyme activity, feeding and body chemical composition of juvenile Chinese mitten crabEriocheir sinensiswere investigated in two independent experiments. Crabs were reared individually during Experiment I and cultured in aggregate during Experiment Ⅱ. Five treatments were involved in both experiments: crabs in the control group (Group C) were fed continuously; crabs in Group S3, S6, S10 and S15 were starved for 3, 6, 10 and 15 days and then re-fed for 3, 6, 10 and 15 days, respectively. Procedures were then repeated. The precocity rate of Group C was significantly higher than that of the other groups. In Experiment I, the final mean body weight and the mean specific growth rate in dry matter (SGRdm) of groups C and S3 was significantly higher than that of the other groups. In Experiment Ⅱ, the mean specific growth rate in body weight (SGRw) in Group C was similar to that of groups S3, S6 and S10, but significantly higher than S15. Crabs in groups S3, S6 and S10 compensated for starving-induced growth depression after being re-fed, and compensatory growth was realized by both higher feeding rate and improved feed efficiency. There was no significant difference in trypsin and alkaline phosphatase activity among treatment groups and the control. However, steapsin and amylase activity were significantly affected by starvation period. There was no significant difference in dry matter, ash and protein content among groups C, S3, S6 and S10 in Experiment I. Collectively, results demonstrated that proper intermittent starvation was sufficient for reducing the precocity rate of cultured juvenileE. sinensis, and we recommend subjecting juvenile crabs to intermittent fasting for 6 to 10 days to maintain precocity rates at acceptable levels without inhibiting growth and yield.