bluuebunny/crossref_metadata_2025_split huggingface dataset:
More or Less: How Do Inhomogeneous Sea‐Salt Aerosols Affect the Precipitation of Landfalling Tropical Cyclones?
Authors: Zhu Limin , Shu Shoujuan , Wang Zheng , Bi Lei 
Published: January 2022

AbstractThe evolution of landfalling Typhoon Fitow in the western North Pacific in 2013 is simulated with an emphasis on the direct radiative effect of sea‐salt aerosols (SSAs) on the precipitation of Fitow. The inhomogeneous SSAs, formed under the environment with low relative humidity (50%–70%), exhibit different optical properties (particular the decreased asymmetry factor) compared with the commonly used homogeneous sea‐salt droplets. We find that incorporating the inhomogeneity of SSAs in the Weather Research and Forecasting (WRF) model decreases the simulated rainfall of Fitow in coastal regions where the continental dry air intrudes. Fundamentally, the cooling effect induced by the particle inhomogeneity and its hysteresis increase atmospheric stability and weaken vertical velocity, which reduce the accumulated rainfall of Fitow. These findings of radiation‐related effects of SSAs provide a new clue for improving the physical processes responsible for precipitation of tropical cyclones in numerical models.
Contributions of Anthropogenic Aerosol Forcing and Multidecadal Internal Variability to Mid‐20th Century Arctic Cooling—CMIP6/DAMIP Multimodel Analysis
Authors: Aizawa Takuro , Oshima Naga , Yukimoto Seiji 
Published: January 2022

AbstractIn the Arctic, observed decadal mean surface air temperatures (SATs) were 0.70°C–0.95°C lower around 1970 than those around 1940. The 35‐multimodel ensemble mean of historical simulations in the Coupled Model Intercomparison Project Phase 6 (CMIP6) exhibited Arctic surface cooling trend in 1940–1970, which could be attributed to external forcings. Multimodel ensemble means of CMIP6 Detection and Attribution Model Intercomparison Project historical simulations exhibited Arctic surface cooling of −0.22°C (±0.24°C) in 1970 versus 1940 and showed that anthropogenic aerosol forcings contributed to a cooling of −0.65°C (±0.37°C), which was partially offset by a warming of 0.44°C (±0.22°C) due to well‐mixed greenhouse gases. In addition to the anthropogenic aerosol forcings, multidecadal internal variability with a magnitude of 0.47°C was the component primarily contributing to the observed Arctic cooling. We identified a spatial pattern of pan‐Arctic multidecadal cooling due to the internal variability that resembles the 1940–1970 cooling pattern.
Electromagnetic Ion Cyclotron Harmonic Waves Generated via Nonlinear Wave‐Wave Couplings
Authors: Deng Dan , Yuan Zhigang , Huang Shiyong , Xue Zuxiang , Huang Zheng , Yu Xiongdong 
Published: January 2022

AbstractIn this letter, we report an electromagnetic ion cyclotron (EMIC) harmonic event observed by the Van Allen Probe B, whose generation is demonstrated to result from nonlinear wave‐wave resonances through the bicoherence analysis. Hybrid simulation shows that the second to sixth harmonics could be excited in sequence after a pump EMIC wave is initially injected, which is in consistent with the observations of EMIC harmonic. It indicates the important role of the second harmonic in the generation of higher harmonics. Finally, the statistical distribution for the amplitude of EMIC second harmonic waves indicates that the energy transfer rate from the fundamental wave to the second harmonic is mainly less than 2%, which might be too low to result in third and other higher harmonics above the observational level. Our results will give some new insights into the excitation of EMIC harmonic waves in the inner magnetosphere.
Impact of Anthropogenic Emission Changes on the Occurrence of Equatorial Plasma Bubbles
Authors: Zhou Xu , Yue Xinan , Ren Zhipeng , Liu Yunbo , Cai Yihui , Ding Feng , Wei Yong 
Published: January 2022

AbstractIn this work, we examine the impact of increased anthropogenic emissions on equatorial plasma bubble (EPB) occurrence by modeling the growth rate of Rayleigh‐Taylor (R‐T) instability (), which is closely related to EPB generation. Using the global coupled ionosphere‐thermosphere‐electrodynamics model‐institute of geology and geophysics, Chinese Academy of Sciences model, is calculated under three different CO2 emission levels. As CO2 increases, significantly increases at low altitudes (<∼260 km) and decreases at high altitudes (>∼320 km). In the altitudes in between, increases (decreases) before (after) midnight. Longitudinal variability of the change is manifested apparently above ∼280 km, while it is insignificant for low altitudes. Term analysis revealed that changes in the gravity term and the electric‐field term are the main causes and that the neutral‐wind term is insignificant. The investigation indicates that increased anthropogenic emissions can change EPB occurrence and, in turn, the radio‐communication system and therefore influence modern technological systems, which is expected to be more serious in the future.
Effect of the Magnetospheric Plasma Interaction and Solar Illumination on Ion Sputtering of Europa’s Surface Ice
Authors: Addison Peter , Liuzzo Lucas , Simon Sven 
Published: January 2022

AbstractFor the entire ion energy range observed at Europa, we calculate spatially resolved maps of the surface sputtering rates of H2O, O2, and H2 from impacts by magnetospheric ions. We use the perturbed electromagnetic fields from a hybrid model of Europa’s plasma interaction, along with a particle‐tracing tool, to calculate the trajectories of magnetospheric ions impinging onto the surface and their resultant sputtering yields. We examine how the distribution of the sputtering rates depends on the electromagnetic field perturbations, the angle between the solar radiation and the corotating plasma flow, and the thickness of the oxygen‐bearing layer within Europa’s surface. Our major findings are: (a) Magnetic field‐line draping partially diverts the impinging ions around Europa, reducing the sputtering rates on the upstream hemisphere, but allowing for substantial sputtering from the downstream hemisphere. In contrast, zero sputtering occurs in much of the downstream hemisphere with uniform electromagnetic fields. (b) If the oxygen‐bearing surface layer is thin compared to the penetration depth of magnetospheric ions, thermal ions dominate the O2 sputtering rates, and the region of strongest sputtering is persistently located near the upstream apex. However, if the oxygen‐bearing layer is thick compared to the penetration depth, energetic ions sputter the most O2, and the location of maximum sputtering follows the sub‐solar point as Europa orbits Jupiter. (c) The global production rate of O2 from Europa’s surface varies by a factor of 3 depending upon the Moon’s orbital position, with the maximum particle release occurring when Europa’s Sun‐lit and upstream hemispheres coincide.
Vertical Transport Timescale of Surface‐Produced Particulate Material in the Chesapeake Bay
Authors: Xiong Jilian , Shen Jian 
Published: January 2022

AbstractAccumulation and remineralization of surface‐produced particulate organic matter (POM) in the water column and seabed link closely to hypoxia and the health of aquatic ecosystems. The POM retention time provides a key timescale to interpret biochemical reaction processes. In this study, we investigated the spatiotemporal variations in the vertical particulate age (VPA) of surface‐produced POM, which is the mean time elapsed since the particulates last contact the surface, by incorporating major physical processes including sinking, resuspension, and deposition in the Chesapeake Bay. It was found that the vertical transport time for the particulates (i.e., VPA) is much longer than the dissolved counterparts as the former consists of new material from the surface and the resuspended aged material that has elongated resting on the seabed after deposition. The VPA is sensitive to settling velocity, especially in low‐frequent resuspension environments, and varies over 2 orders of magnitude with settling velocity from 0 to 10 m/day. Slow‐sinking material can remain in suspension and seldom settle to the seabed, thus mainly contribute to pelagic processes, while the fast‐sinking material connects closely with benthic processes. The seasonality of VPA decreases as the settling velocity increases. No significant difference in VPA was found between wet and dry years, yet the episodic strong flood events entrain old materials from the depositional lateral shoals to increase VPA in the channel. The transport age bridges cross disciplinaries by providing the fourth‐dimensional age information as a common currency to compare the physical transport timescale with the timescales for biochemical reactions.
Electron Dynamics in a Chorus Wave Field Generated From Particle‐In‐Cell Simulations
Authors: An Zeyu , Wu Yifan , Tao Xin 
Published: January 2022

AbstractHow to properly describe resonant interactions between electrons and quasi‐coherent chorus waves is still an open question. Previous studies have progressed from modeling chorus as a single wave to considering effects such as amplitude modulation or phase decoherence in wave particle resonance. However, incorporating realistic features of chorus waves in test particle calculations has always been a challenging but critically important step to evaluate their nonlinear effects. In this work, we use a rising‐tone chorus element generated by a particle‐in‐cell simulation in test‐particle simulations. The used chorus wave naturally has features including amplitude modulation, phase decoherence and dynamic evolution during propagation. Our results suggest that, while being latitudinal dependent, realistic features of chorus generally lead to significant suppression of nonlinear effects. This result should be important to understand phase space transport of electrons due to interactions with chorus waves.
Dust Rotation and Swirl Morphology in Lunar Magnetic Anomalies
Authors: Gerard Michael , Deca Jan , Horányi Mihály 
Published: January 2022

AbstractThe scattering of light off the bright regions of lunar swirls suggests a relatively higher fraction of fine‐grained dust compared to dust with similar optical maturity values found elsewhere on the lunar surface when assuming an isotropically oriented dust distribution, without preferred grain orientation. Here we propose a mechanism by which the ferromagnetic lunar fines, lofted by electrostatic effects or by meteoroid impacts, may rotate in a lunar magnetic anomaly and, upon landing, produce patches of organized alignments, generating anisotropic surface structures. We simulate these rotations for Reiner Gamma and provide a proof of concept for such a dust rotation model. While the magnetic forces remain negligibly small to influence the trajectories of the lofted particles, the magnetic torques have a significant effect on the rotation of magnetized dust, and can generate an organized landing pattern for elongated lunar fines, which offers a possible explanation for the lunar albedo patterns.
Targeting a splicing-mediated drug resistance mechanism in prostate cancer by inhibiting transcriptional regulation by PKCβ1
Authors: Melnyk James E. , Steri Veronica , Nguyen Hao G. , Hwang Y. Christina , Gordan John D. , Hann Byron , Feng Felix Y. , Shokat Kevan M. 
Published: January 2022

AbstractThe androgen receptor (AR) is a central driver of aggressive prostate cancer. After initial treatment with androgen receptor signaling inhibitors (ARSi), reactivation of AR signaling leads to resistance. Alternative splicing of AR mRNA yields the AR-V7 splice variant, which is currently an undruggable mechanism of ARSi resistance: AR-V7 lacks a ligand binding domain, where hormones and anti-androgen antagonists act, but still activates AR signaling. We reveal PKCβ as a druggable regulator of transcription and splicing at the AR genomic locus. We identify a clinical PKCβ inhibitor in combination with an FDA-approved anti-androgen as an approach for repressing AR genomic locus expression, including expression of AR-V7, while antagonizing full-length AR. PKCβ inhibition reduces total AR gene expression, thus reducing AR-V7 protein levels and sensitizing prostate cancer cells to current anti-androgen therapies. We demonstrate that this combination may be a viable therapeutic strategy for AR-V7-positive prostate cancer.
NEAR trial: A single-arm phase II trial of neoadjuvant apalutamide monotherapy and radical prostatectomy in intermediate- and high-risk prostate cancer
Authors: Lee Lui Shiong , Sim Adelene Y. L. , Ong Chee Wee , Yang Xinyan , Ng Cedric C. Y. , Liu Wei , Rajasegaran Vikneswari , Lim Abner M. S. , Aslim Edwin Jonathan , Ngo Nye-Thane , Khor Li-Yan , Kanesvaran Ravindran , Allen John Carson Jr , Tay Kae Jack , Yuen John Shyi Peng , Chong Tsung Wen , Ho Sun Sien Henry , Teh Bin Tean , Chua Melvin L. K. 
Published: January 2022

Abstract
Objective
Treatment efficacy of androgen deprivation therapy with radical prostatectomy for intermediate- to high-risk prostate cancer is less well-studied. The NEAR trial is a single-arm, phase II investigation of neoadjuvant apalutamide monotherapy and radical prostatectomy (RP) in the treatment of D’Amico intermediate- and high-risk prostate cancer (NCT03124433).

Materials and methods
Patients with histologically-proven, D’Amico intermediate- to high-risk prostate adenocarcinoma received apalutamide 240 mg once-daily for 12 weeks followed by RP + /−lymphadenectomy. Primary outcome was pathological complete response (pCR) rate. Secondary outcomes included rate of biochemical response (defined by PSA < 0.03 ng/mL at week 24 from starting apalutamide without subsequent PSA relapse), treatment-related adverse events, and RP complication rates. Correlative biomarker analyses were performed to examine for molecular predictors of treatment responses.

Results
From 2017 to 2019, 30 patients were recruited, of which 20 and 10 were high and intermediate risk, respectively; 25 completed treatment as per-protocol. We did not observe any pCR on trial; median reduction of cancer burden was 41.7% (IQR: 33.3%–60.0%). 18 out of 25 patients were classified as having a biochemical response (4 did not achieve PSA of <0.03 ng/mL at week 24 and 3 developed PSA relapse subsequently). Dry skin (N = 16; 53.3%), fatigue (N = 10; 33.3%) and skin rash (N = 9; 30.0%) were the most common adverse events, and there was no major peri-operative complication. We observed an association between tumours of low androgen receptor activity and PAM50 basal status with biochemical non-responders, albeit these molecular phenotypes were not associated with pathological response.

Conclusions
A 12-week course of neoadjuvant apalutamide prior to RP did not meet the primary endpoint of pCR in this trial. Tumours with low androgen receptor activity or of the PAM50 basal subtype may have a reduced response to apalutamide.
Phylogenetic divergence and adaptation of Nitrososphaeria across lake depths and freshwater ecosystems
Authors: Ren Minglei , Wang Jianjun 
Published: January 2022

Abstract
Thaumarchaeota (now the class Nitrososphaeria in the phylum Thermoproteota in GTDB taxonomy) are abundant across marine and soil habitats; however, their genomic diversity and evolutionary history in freshwater environments remain elusive. Here, we reconstructed 17 high-quality metagenome-assembled genomes of Nitrososphaeria from a deep lake and two great rivers, and compared all available genomes between freshwater and marine habitats regarding their phylogenetic positions, relative abundance, and genomic content. We found that freshwater Nitrososphaeria were dominated by the family Nitrosopumilaceae and could be grouped into three distinct clades closely related to the genera Nitrosopumilus, Nitrosoarchaeum, and Nitrosotenuis. The Nitrosopumilus-like clade was exclusively from deep lakes, while the Nitrosoarchaeum-like clade was dominated by species from deep lakes and rivers, and the Nitrosotenuis-like clade was mainly from rivers, deep lakes, and estuaries. Interestingly, there was vertical niche separation between two clades in deep lakes, showing that the Nitrosopumilus-like species dominated shallow layers, whereas the relative abundance of the Nitrosoarchaeum-like clade increased toward deep waters. Phylogenetic clustering patterns in the Nitrosopumilaceae supported at least one freshwater-to-marine and two marine-to-freshwater transitions, the former of which refined the potential terrestrial-to-marine evolutionary path as previously proposed. The occurrence of the two marine-to-freshwater transitions were accompanied by horizontal transfer of the genes involved in nutrition regulation, osmoregulation, and cell motility during their colonization to freshwater habitats. Specifically, the Nitrosopumilus-like clade showed losses of genes encoding flagella assembly and ion transport, whereas the Nitrosoarchaeum-like clade had losses of intact genes involved in urea uptake and utilization and gains of genes encoding osmolarity-mediated mechanosensitive channels. Collectively, our results reveal for the first time the high genomic diversity of the class Nitrososphaeria across freshwater ecosystems and provide novel insights into their adaptive mechanisms and evolutionary histories.
Mapping the expression of an ANK3 isoform associated with bipolar disorder in the human brain
Authors: Holmgren Asbjørn , Hansson Lars , Bjerkaas-Kjeldal Kristine , Impellizzeri Agata Antonia Rita , Gilfillan Gregor D. , Djurovic Srdjan , Hughes Timothy 
Published: January 2022

AbstractThe gene ankyrin-3 (ANK3) has been consistently associated with bipolar disorder (BD) in several genome-wide association studies (GWAS). The exact molecular mechanisms underlying this genetic association remain unknown. The discovery of a loss-of-function variant (rs41283526*G) in an alternatively spliced exon (ENSE00001786716) with a protective effect, suggested that elevated expression of this particular isoform could be a risk factor for developing the disorder. We developed a novel approach for measuring the expression level of all splice forms at a challenging genetic locus using a combination of droplet digital PCR and high-throughput sequencing of indexed PCR amplicons. The combined method was performed on a large collection of 568 postmortem brain samples of BD and SCZ cases and controls. We also studied the expression of the splice forms in a child-development cohort of 41 healthy males. We found that our approach can quantify the splice forms in brain samples, although with less precision than ddPCR. We detected highly significant differences in expression of splice forms and transcription start sites between brain regions, notably with higher expression of the BD-associated isoform in the corpus callosum compared to frontal tissue (mean fold change = 1.80, p < 1e-4). Although the patients in our sample expressed the BD-associated splice form at a similar level to controls, adolescents in our child-development cohort had a clearly higher expression level than younger children (mean fold change = 1.97, p = 5e-3). These results suggest that this ANK3 splice form may play a role in the myelin maturation of the human brain.
Brain network dysfunctions in addiction: a meta-analysis of resting-state functional connectivity
Authors: Tolomeo Serenella , Yu Rongjun 
Published: January 2022

AbstractResting-state functional connectivity (rsFC) provides novel insights into variabilities in neural networks associated with the use of addictive drugs or with addictive behavioral repertoire. However, given the broad mix of inconsistent findings across studies, identifying specific consistent patterns of network abnormalities is warranted. Here we aimed at integrating rsFC abnormalities and systematically searching for large-scale functional brain networks in substance use disorder (SUD) and behavioral addictions (BA), through a coordinate-based meta-analysis of seed-based rsFC studies. A total of fifty-two studies are eligible in the meta-analysis, including 1911 SUD and BA patients and 1580 healthy controls. In addition, we performed multilevel kernel density analysis (MKDA) for the brain regions reliably involved in hyperconnectivity and hypoconnectivity in SUD and BA. Data from fifty-two studies showed that SUD was associated with putamen, caudate and middle frontal gyrushyperconnectivityrelative to healthy controls. Eight BA studies showedhyperconnectivityclusters within the putamen and medio-temporal lobe relative to healthy controls. Alteredconnectivityin salience or emotion-processing areas may be related to dysregulated affective and cognitive control-related networks, such as deficits in regulating elevated sensitivity to drug-related stimuli. These findings confirm that SUD and BA might be characterized by dysfunctions in specific brain networks, particularly those implicated in the core cognitive and affective functions. These findings might provide insight into the development of neural mechanistic biomarkers for SUD and BA.
TRK-fused gene (TFG) regulates ULK1 stability via TRAF3-mediated ubiquitination and protects macrophages from LPS-induced pyroptosis
Authors: Shi Jian-Hong , Ling Chen , Wang Ting-Ting , Zhang Li-Nuo , Liu Wen-Wen , Qin Yan , Tan Ying-Hui , Cui Nai-Peng , Ni Zhi-Yu 
Published: January 2022

AbstractTRK-fused gene (TFG) is known to be involved in protein secretion and plays essential roles in an antiviral innate immune response. However, its function in LPS-induced inflammation and pyroptotic cell death is still unknown. Here, we reported that TFG promotes the stabilization of Unc-51 like autophagy activating kinase (ULK1) and participates in LPS plus nigericin (Ng) induced pyroptotic cell death. Our results showed that TFG-deficient THP-1 macrophages exhibit higher mitochondrial ROS production. LPS/Ng stimulation triggers a much higher level of ROS and induces pyroptotic cell death. ULK1 undergoes a rapid turnover in TFG-deficient THP-1 cells. TFG forms complex with an E3 ligase, tumor necrosis factor receptor-associated factor 3 (TRAF3), and stabilizes ULK1 via disturbing ULK1-TRAF3 interaction. Knockdown of TFG facilitates the interaction of ULK1 with TRAF3 and subsequent K48-linked ULK1 ubiquitination and proteasome degradation. Rescue of ULK1 expression blocks LPS/Ng-induced cell death in TFG-deficient THP-1 macrophages. Taken together, TFG plays an essential role in LPS/Ng-induced pyroptotic cell death via regulating K48-linked ULK1 ubiquitination in macrophages.
Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk
Authors: Twigger Alecia-Jane , Engelbrecht Lisa K. , Bach Karsten , Schultz-Pernice Isabel , Pensa Sara , Stenning Jack , Petricca Stefania , Scheel Christina H. , Khaled Walid T. 
Published: January 2022

AbstractUnder normal conditions, the most significant expansion and differentiation of the adult mammary gland occurs in response to systemic reproductive hormones during pregnancy and lactation to enable milk synthesis and secretion to sustain the offspring. However, human mammary tissue remodelling that takes place during pregnancy and lactation remains poorly understood due to the challenge of acquiring samples. We report here single-cell transcriptomic analysis of 110,744 viable breast cells isolated from human milk or non-lactating breast tissue, isolated from nine and seven donors, respectively. We found that human milk largely contains epithelial cells belonging to the luminal lineage and a repertoire of immune cells. Further transcriptomic analysis of the milk cells identified two distinct secretory cell types that shared similarities with luminal progenitors, but no populations comparable to hormone-responsive cells. Taken together, our data offers a reference map and a window into the cellular dynamics that occur during human lactation and may provide further insights on the interplay between pregnancy, lactation and breast cancer.