bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving fresh cheese shelf‐life through hyperbaric storage at variable room temperature
Authors: Duarte Ricardo V. , Lopes‐da‐Silva José A. , Gomes Ana M. , Delgadillo Ivonne , Barba Francisco J. , Saraiva Jorge A. 
Published: December 2022

AbstractThe changes in microbiological, physiochemical, and textural properties in fresh cheeses made from either cow or goat milk were observed under hyperbaric storage (HS, 50–100 MPa) at room temperature (RT) and compared with refrigerated storage under normal atmospheric pressure for 60 days. An initial microbial growth inhibition was observed for both cheese types, as well as a considerable inactivation of all endogenous microbiota under HS/RT (75–100 MPa/RT). This contributed to a higher stability of pH and color values, especially at the higher pressure at room temperature (100 MPa/RT) throughout 60 days storage. A compression effect occurred during HS/RT, resulting in higher whey loss, reduction in moisture content, and textural changes. Such changes tended to decrease over time, to values closer to the initial ones, with hardness values at the 60th day of storage at 75/RT similar to those observed for refrigeration on the 7th day and 1.4‐fold higher than those observed at 100/RT. Overall, HS/RT reduced the microbial populations load during storage (≥5 log units in some cases), with minimal effects on most of the evaluated quality parameters. These results point to a considerable shelf‐life extension of HS fresh cheeses, without temperature control, pinpointing HS as a more sustainable preservation strategy than refrigeration, with great potential for industrial application.Practical ApplicationThe results presented in this study point to increased microbial stability of fresh cheeses when stored under hyperbaric storage without temperature control, leading possibly to an increased shelf‐life, of up to 60 days. This kind of new food preservation strategy may be suitable for longer transportation of foods, where energy may not be handily and widely available, while additionally contributing to increased shelf‐life and safety. Also, hyperbaric storage could be applied throughout the food storage, improving shelf‐life with a lower carbon footprint than refrigeration.
Microbial community and extracellular polymeric substances analysis of anaerobic granular sludge exposed to selenate, cadmium and zinc
Authors: Zeng Taotao , Hu Qing , Rene Eldon R. , Lens Piet N. L. 
Published: December 2022

AbstractThe microbial community and extracellular polymeric substances composition of anaerobic granular sludge exposed to selenate (~10 mg/L), cadmium (Cd) and zinc (Zn) (~2 and 5 mg/L) were investigated by high‐throughput sequencing and fluorescence excitation emission matrix (FEEM) spectra, respectively. As a response to selenate, Cd and/or Zn exposure, significant fluorescence quenching of fulvic‐like acids and humic‐like substances was observed. With selenate, Cd and/or Zn in the influent with respective concentrations of 10, 5 and 5 mg/L, the abundance of the phyla Proteobacteria, Firmicutes, Spirochaetae, Cloacimonetes and Synergistetes increased significantly, and the dominant taxa in the anaerobic granular sludge exposed to Se, Cd and/or Zn were Halothiobacillaceae (10.2%), Pseudomonas (8.8%), Synergistaceae (7.7%), Spirochaetaceae (7.2%), Blvii28 wastewater sludge group (6.7%), Telmatospirillum (4.6%), Veillonellaceae (4.3%), Geobacter (4.0%) and Enterobacteriaceae (3.0%). Compared with the inoculum, the abundance of the archaea Methanobacterium and Methanosaeta decreased to below detection limit in the UASB reactor after 116 days exposure to Se, Cd and Zn.
Two single nucleotide variants in the miR‐23a promoter affect granulosa cell apoptosis
Authors: Wang Siqi , Li Yuqi , Du Xing , Li Qifa 
Published: December 2022

AbstractmicroRNAs (miRNAs) are well known to be important in mammalian female fertility. However, the genetic regulation of miRNAs associated with female fertility remains largely unknown. Here, we report that two single‐nucleotide variants (SNVs) in the miR‐23a promoter strongly influence miR‐23a transcription and function in granulosa cell (GC) apoptosis. Two novel SNVs, g.‐283G>C and g.‐271C>T, were detected in the porcine miR‐23a promoter by pooled‐DNA sequencing. Furthermore, SNVs in the promoter region influenced miR‐23a transcription in porcine GCs by altering its promoter activity. Functionally, SNVs in the promoter strongly influenced miR‐23a regulation of early apoptosis in porcine GCs cultured in vitro. In addition, a preliminary association analysis showed that the combined genotypes of the two SNVs, rather than a single SNV, were tentatively associated with sow fertility traits in a Large White population. Overall, our findings suggest that the SNVs g.‐283G>C and g.‐271C>T in the miR‐23a promoter are causal variants affecting GC apoptosis and miR‐23a may be a potential small‐molecule nonhormonal drug for regulating female fertility.
The need of having a plan in excessive infant crying – A qualitative study of parents' experiences of healthcare support
Authors: Harskamp‐Van Ginkel Margreet W. , Klazema Willemien , Hoogsteder Mariëtte H. H. , Chinapaw Mai J. M. , van Houtum Lieke 
Published: December 2022

AbstractAimExcessive infant crying increases parents' concerns regarding their infant's health and the burden of parenting. We aimed to gain insight into the healthcare support needs of parents with excessively crying infants.MethodsAn exploratory qualitative study was conducted in the Netherlands. We performed semi‐structured interviews with parents of 12 infants between June and December 2020, followed by inductive and deductive thematic analysis.ResultsParents described what their needs were with regard to the assessment of infant crying and support by professionals. Long‐lasting crying made parents feel that there must be a somatic cause. If they could soothe their infant, they gained more confidence that their infant was healthy. We identified four interrelated themes: (i) confidence in the professional; (ii) seeking a somatic cause for the crying; (iii) seeking acknowledgment; and (iv) exhaustion of parents and feelings of failure.ConclusionParental support needs were best fulfilled by professionals who took them seriously, demonstrated medical expertise, and offered a practical plan. Perinatal parental education on normal infant behaviour and infant soothing techniques might improve parental self‐efficacy at an early stage and prevent medicalization of excessive crying.
Understanding and overcoming challenges in times of personal or global crisis—Editorial on the Special Issue on Loneliness and Health
Authors: Lippke Sonia , Warner Lisa Marie 
Published: December 2022

AbstractThe ever‐present interest in loneliness has increased during the last decade. Although loneliness is generally not as prevalent as other topics and not among the top 5 most read papers in the journal Applied Psychology: Health and Well‐Being, it is closely connected to topics therein, such as well‐being and health. Conceptualizing loneliness as indicator of risk, it may function as a cue for action. Accordingly, understanding loneliness, its development, prevalence, effects, and how to support individuals to prevent or overcome loneliness is key and the main aim of this special issue. Therefore, theories and models are reviewed in this paper and synthesized together with other aspects relating to the field of loneliness research and intervention. Accordingly, we propose an agenda including key determinants (e.g., risk groups), how to proceed (various research methods), intervention components (e.g., behavior change techniques, SDGs), and how to perform dissemination (open science practices, co‐creative approaches, etc.). The original studies in this special issue provide stimulating examples. Moreover, the commentaries give new insights and inspiring ideas. Overall, this special issue aims to give readers a lens with which to re‐examine their own research, enable innovation, and empower addressing loneliness and its interconnection synergistically.
“I had no power whatsoever”: Graduate student survivors’ experiences disclosing sexual harassment to mandatory reporters
Authors: Cipriano Allison E. , Holland Kathryn J. , Rieger Agnes , O'Callaghan Erin 
Published: December 2022

AbstractMany universities have implemented mandatory reporting policies requiring most employees to report sexual harassment to the Title IX office, even if the victim/survivor does not want them to. Such policies constrain survivor autonomy and are particularly relevant for graduate students, who work closely with designated mandated reporter employees. This study examined graduate student sexual harassment survivors’ experiences disclosing to mandatory reporters, including interactions with mandatory reporters and the outcomes of their reports. We conducted interviews with graduate student survivors whose experiences were reported to their university's Title IX office and analyzed data from the cases involving a mandated report. Using thematic analysis, we identified three themes: survivors lacked knowledge that they were disclosing to a mandatory reporter, mandatory reporters lacked knowledge about Title IX processes, and mandatory reporters provided support. When analyzing outcomes of mandated reports, most did not result in formal grievance procedures, and the few that were investigated and adjudicated did not result in meaningful sanctions. Accommodations were rarely used, because they were not offered, were not perceived as helpful, or their requests were denied. Findings suggest that the purported goals of mandatory reporting policies were not met and that polices should afford survivors more autonomy.
Role of hydrogen sulphide in physiological and pathological angiogenesis
Authors: Zhang Yan‐Xia , Jing Mi‐Rong , Cai Chun‐Bo , Zhu Shuai‐Gang , Zhang Chao‐Jing , Wang Qi‐Meng , Zhai Yuan‐Kun , Ji Xin‐Ying , Wu Dong‐Dong 
Published: December 2022

AbstractThe role of hydrogen sulphide (H2S) in angiogenesis has been widely demonstrated. Vascular endothelial growth factor (VEGF) plays an important role in H2S‐induced angiogenesis. H2S promotes angiogenesis by upregulating VEGF via pro‐angiogenic signal transduction. The involved signalling pathways include the mitogen‐activated protein kinase pathway, phosphoinositide‐3 kinase pathway, nitric oxide (NO) synthase/NO pathway, signal transducer and activator of transcription 3 (STAT3) pathway, and adenosine triphosphate (ATP)‐sensitive potassium (KATP) channels. H2S has been shown to contribute to tumour angiogenesis, diabetic wound healing, angiogenesis in cardiac and cerebral ischaemic tissues, and physiological angiogenesis during the menstrual cycle and pregnancy. Furthermore, H2S can exert an anti‐angiogenic effect by inactivating Wnt/β‐catenin signalling or blocking the STAT3 pathway in tumours. Therefore, H2S plays a double‐edged sword role in the process of angiogenesis. The regulation of H2S production is a promising therapeutic approach for angiogenesis‐associated diseases. Novel H2S donors and/or inhibitors can be developed in the treatment of angiogenesis‐dependent diseases.
Recommended practices and ethical considerations for natural language processing‐assisted observational research: A scoping review
Authors: Fu Sunyang , Wang Liwei , Moon Sungrim , Zong Nansu , He Huan , Pejaver Vikas , Relevo Rose , Walden Anita , Haendel Melissa , Chute Christopher G. , Liu Hongfang 
Published: December 2022

AbstractAn increasing number of studies have reported using natural language processing (NLP) to assist observational research by extracting clinical information from electronic health records (EHRs). Currently, no standardized reporting guidelines for NLP‐assisted observational studies exist. The absence of detailed reporting guidelines may create ambiguity in the use of NLP‐derived content, knowledge gaps in the current research reporting practices, and reproducibility challenges. To address these issues, we conducted a scoping review of NLP‐assisted observational clinical studies and examined their reporting practices, focusing on NLP methodology and evaluation. Through our investigation, we discovered a high variation regarding the reporting practices, such as inconsistent use of references for measurement studies, variation in the reporting location (reference, appendix, and manuscript), and different granularity of NLP methodology and evaluation details. To promote the wide adoption and utilization of NLP solutions in clinical research, we outline several perspectives that align with the six principles released by the World Health Organization (WHO) that guide the ethical use of artificial intelligence for health.
Objective sleep characteristics and continuous glucose monitoring profiles of type 2 diabetes patients in real‐life settings
Authors: Zhao Yan , Zheng Yuchan , Tian Yixin , Yu Qian , Qin Lijun , Xu Kai , Sun Biao , Benedict Christian , Chen Baoyi , Wei Lijun , Tan Xiao 
Published: December 2022

AbstractAimTo investigate the association between objective sleep parameters and glycaemic variability determined by continous glucose monitoring (CGM) among patients with type 2 diabetes, given the significant role of sleep in glycaemic control.MethodsIn this study, CGM was carried out in 28 patients with T2D (aged 62.3 ± 4.8 years, 57% women). Sleep characteristics were assessed by actigraphy within the CGM period. CGM‐derived outcomes included glucose level, and percentages of time in range (TIR) and time above range (TAR) during the monitoring period. Associations between intraindividual night‐to‐night variations in sleep characteristics and overall CGM outcomes were analysed using linear regression. Associations between sleep characteristics during each night and time‐matched CGM outcomes were analysed using linear mixed models.ResultsA total of 249 person‐days of CGM, coupled with 221 nights of sleep characteristics, were documented. Greater standard deviation (SD) of objective sleep duration (minutes) between measurement nights was associated with higher glucose level (coefficient 0.018 mmol/L [95% confidence interval {CI} 0.004, 0.033], P = 0.017), smaller proportion of TIR (% in observation period; coefficient −0.20% [95% CI −0.36, −0.03], P = 0.023), and greater proportion of TAR (coefficient 0.22% [95% CI 0.06, 0.39], P = 0.011). Later sleep midpoint (minutes from midnight) was associated with greater SD of glucose during the same sleep period (coefficient 0.002 minutes [95% CI 0.0001, 0.003], P = 0.037), longer nocturnal sleep duration was associated with smaller coefficient of variation of glucose level in the upcoming day (−0.015% [95% CI −0.03, −0.001], P = 0.041).ConclusionObjectively determined sleep duration and sleep midpoint, as well as their daily variability, are associated with CGM‐derived glucose profiles in T2D patients.
Normal values for superior vena cava flow in preterm neonates using a new echocardiographic approach
Authors: Riccardi Riccardo , Barone Giovanni , Iannotta Rossella , Prontera Giorgia , D'Andrea Vito , Vento Giovanni 
Published: December 2022

AbstractObjectiveRecently, a novel approach to imaging Superior Vena Cava (SVC) flow has been presented, showing better repeatability and better agreement with MRI‐derived SVC flow measures. The objective was to establish normal values of SVC flow with the novel approach in the first 48 h of life.Study DesignThis was a prospective, observational study. All infants with gestational age (GA) less than 31 weeks were eligible. Echocardiographic evaluation was performed at 5, 12, 24, 48 h of postnatal life. A subgroup of uncomplicated infants was studied to define a normal range for SVC flow.ResultsForty‐five infants were enrolled. We estimated normative values in a subgroup of 31 uncomplicated infants. The median SVC flow significantly increases from 83 ml/kg/min at 5 h of life to 153 ml/kg/min at 48 h (p < .001).ConclusionUsing the novel approach we derived normal values of SVC flow in a cohort of uncomplicated preterm population at high risk for developing IVH.
The APOE4 effect: structural brain differences in Alzheimer's disease according to the age at symptom onset
Authors: Forno Gonzalo , Contador Jose , Pérez‐Millan Agnès , Guillen Nuria , Falgàs Neus , Sarto Jordi , Tort‐Merino Adrià , Castellví Magdalena , Bosch Beatriz , Fernández‐Villullas Guadalupe , Balasa Mircea , Antonell Anna , Sala‐ Llonch Roser , Sanchez‐Valle Raquel , Hornberger Michael , Lladó Albert 
Published: December 2022

AbstractBackground and purposeHow the APOE genotype can differentially affect cortical and subcortical memory structures in biomarker‐confirmed early‐onset (EOAD) and late‐onset (LOAD) Alzheimer's disease (AD) was assessed.MethodEighty‐seven cerebrospinal fluid (CSF) biomarker‐confirmed AD patients were classified according to their APOE genotype and age at onset. 28 were EOAD APOE4 carriers (+EOAD), 21 EOAD APOE4 non‐carriers (–EOAD), 23 LOAD APOE4 carriers (+LOAD) and 15 LOAD APOE4 non‐carriers (–LOAD). Grey matter (GM) volume differences were analyzed using voxel‐based morphometry in Papez circuit regions. Multiple regression analyses were performed to determine the relation between GM volume loss and cognition.ResultsSignificantly more mammillary body atrophy in +EOAD compared to –EOAD is reported. The medial temporal and posterior cingulate cortex showed less GM in +LOAD compared to –LOAD. Medial temporal GM volume loss was also found in +EOAD compared to –LOAD. With an exception for +EOAD, medial temporal GM was strongly associated with episodic memory in the three groups, whilst posterior cingulate cortex GM volume was more related with visuospatial abilities. Visuospatial abilities and episodic memory were also associated with the anterior thalamic nucleus in –LOAD.ConclusionsOur results show that the APOE genotype has a significant effect on GM integrity as a function of age of disease onset. Specifically, whilst LOAD APOE4 genotype is mostly associated with increased medial temporal and parietal atrophy compared to –LOAD, for EOAD APOE4 might have a more specific effect on subcortical (mammillary body) structures. The findings suggest that APOE genotype needs to be taken into account when classifying patients by age at onset.
Clinical outcomes of delayed mechanical thrombectomy: Descriptive analysis and development of a screening tool
Authors: Berry‐Noronha Alexander , Myall Daniel , Hong Jae Beom , Collecutt Wayne , Krauss Martin , Fink John , Weggery Susan , Chatterjee Animesh , Bartholomew Sam , Smith Mark , Le Heron Campbell , Busby Wendy , Brew Stefan , Barber Peter Alan , Wu Teddy Y. , Wilson Duncan 
Published: December 2022

AbstractBackground and purposeLimited data guide the selection of patients with large vessel occlusion ischaemic stroke who may benefit from referral to a distant tertiary centre for mechanical thrombectomy (MT). We aimed to characterize this population, describe clinical outcomes and develop a screening system to identify patients most likely to benfit from delayed mechanical thrombectomy (MT).MethodsWe undertook a retrospective cohort analysis enrolling patients transferred from regional sites to one of two MT comprehensive stroke units with a time from non‐contrast computed tomography (NCCT) of the brain to reperfusion of 4 h or more. We describe Alberta Stroke Programme Early Computed Tomography Score (ASPECTS), National Institute of Health Stroke Scale (NIHSS) and modified Rankin Scale (mRS) in our patients and compare these patients to those in extended‐time‐window trials. Lastly, we developed and validated a scoring model to help clinicians identify appropriate patients based on variables associated with poor outcomes.ResultsWe included 563 patients, 46% of whom received thrombolysis; the median (interquartile range [IQR]) ASPECTS was 8 (7–10) and the median (IQR) NIHSS score was 16 (11–20). The median (IQR) symptom to mechanical reperfusion time was 390 (300–580) min. Eight patients (1%) had a symptomatic haemorrhage. We achieved good clinical outcome (defined as mRS score ≤2) in 299 patients (54%). Age, diabetes, NIHSS score and ASPECTS were used to create a weighted scoring system with a validated area under the curve of 0.83 (95% confidence interval 0.74–0.92).ConclusionOur study shows, in highly selected patients, that delayed MT many hours after baseline NCCT is associated with good clinical outcomes. However, older patients with diabetes, high NIHSS score and low ASPECTS may not benefit from transfer to a hub centre many hours away for MT in this model of care.
Using landscape genomics to delineate future adaptive potential for climate change in the Yosemite toad (Anaxyrus canorus)
Authors: Maier Paul A. , Vandergast Amy G. , Bohonak Andrew J. 
Published: December 2022

AbstractAn essential goal in conservation biology is delineating population units that maximize the probability of species persisting into the future and adapting to future environmental change. However, future‐facing conservation concerns are often addressed using retrospective patterns that could be irrelevant. We recommend a novel landscape genomics framework for delineating future “Geminate Evolutionary Units” (GEUs) in a focal species: (1) identify loci under environmental selection, (2) model and map adaptive conservation units that may spawn future lineages, (3) forecast relative selection pressures on each future lineage, and (4) estimate their fitness and likelihood of persistence using geo‐genomic simulations. Using this process, we delineated conservation units for the Yosemite toad (Anaxyrus canorus), a U.S. federally threatened species that is highly vulnerable to climate change. We used a genome‐wide dataset, redundancy analysis, and Bayesian association methods to identify 24 candidate loci responding to climatic selection (R2ranging from 0.09 to 0.52), after controlling for demographic structure. Candidate loci included genes such as MAP3K5, involved in cellular response to environmental change. We then forecasted future genomic response to climate change using the multivariate machine learning algorithm Gradient Forests. Based on all available evidence, we found three GEUs in Yosemite National Park, reflecting contrasting adaptive optima: YF‐North (high winter snowpack with moderate summer rainfall), YF‐East (low to moderate snowpack with high summer rainfall), and YF‐Low‐Elevation (low snowpack and rainfall). Simulations under the RCP 8.5 climate change scenario suggest that the species will decline by 29% over 90 years, but the highly diverse YF‐East lineage will be least impacted for two reasons: (1) geographically it will be sheltered from the largest climatic selection pressures, and (2) its standing genetic diversity will promote a faster adaptive response. Our approach provides a comprehensive strategy for protecting imperiled non‐model species with genomic data alone and has wide applicability to other declining species.