bluuebunny/crossref_metadata_2025_split huggingface dataset:
Socio Demographic Profile and HER2 Status in Patients with Colorectal Carcinoma
Authors: Khanam Shamima , Khastagir Tirtha , Anjum Rahat , Barua Mitasree , Khatun Monira , Rahman Mohammed Zillur 
Published: May 2022

Background : Colorectal carcinoma (CRC) is the most frequently observed malignancy worldwide. The incidence of CRC is 3.5% in Bangladesh. Conflicting data exist about the prevalence of HER-2/neu over expression in colorectal cancer ranging from 0 to 83%. The aim of this study was to evaluate the expression of Her-2/neu protein in colorectal cancers.
Materials and methods: This descriptive cross-sectional study was conducted in the Department of Pathology of Chittagong Medical College, Chittagong, Bangladesh from 1st January 2017 to 31st December 2017. A total of 52 cases with a histopathological diagnosis of colorectal cancers included in the study as sample size, HER2 status was evaluated by immunohistochemistry (IHC) in formalin fixed, paraffin embedded tissue.Positive expression was assigned a score from 0–3+.IHCs were analysed with grades of colon cancers.
Results: The mean age in this study was 47.62years range from 12 to 85 years ,53.8% were female. Among 52 specimens 30 (57.7%) cases were from colon and all the cases were adenocarcinoma and rest of all (42.3%) were from rectum.33 (63.5%) cases were Grade-I, 8 (15.4%) were Grade-II, and 11 (21.2%) were Grade-III. Only 8 (15.4%) of 52 cases showed HER2 positive, 4 (50.0%) cases were from grade- Iand another 4 (50.0%) were grade- II tumours. HER2 overexpression were significantly associated with well differentiated CRC than poorly differentiated (p=0.044).
Conclusion: HER2/neu protein expression was observed in colorectal cancer but HER-2/neu protein is less likely to be expressed in colon cancer cell lines among our patients.
Chatt Maa Shi Hosp Med Coll J; Vol.21 (1); January 2022; Page 52-56
Evaluation of Maternal and Neonatal Outcomes in Pregnant Women with Placenta Previa and Placenta Accreta
Authors: Laizu Ismat Ara , Banu Laila Anjuman , Fayez Fowzia Abul 
Published: May 2022

Background: Placenta praevia is a disorder that happens during pregnancy when the placenta is abnormally placed in the lower uterine segment, which at times covers the cervix. Placenta previa may be associated with placenta accreta (PA) or one of its more advanced forms as (placenta increta and percreta).
Objective: The purpose of the present study was to evaluate the maternal and neonatal outcomes in patients with placenta previa and placenta accrete.
Methodology: This prospective descriptive study was carried out at Different privet chamber in Dhaka City, during study period from January 2018 to December 2019. Among 75 cases (45 had placenta previa and 30 had placenta accrete) diagnosed preoperatively by ultrasound or postoperatively with or without PA. Maternal and neonatal outcomes were evaluated. All intraoperative and postoperative data were reported. The obtained data was analyzed by means of SPSS software (version 23.0) and p<0.05 was taken as the significant level.
Results: Cesarean hysterectomy, Urinary tract injuries, EBL, Patients receiving mean transfusion , mean Operative time, Admission to maternity HDU, Admission to ICU and mean Postoperative hospital stay (days) statistically significant (p<0.05), however age, parity and gestational age was not statistically these were significant (p>0.05) between two groups. IUFD was found 1(3.3%) in placenta accrete group but not found in placenta previa group. Neonatal death was found 1(3.3%) in placenta accrete group but not found in placenta previa group.
Conclusion: The incidence of both PP and PA is very high in present locality due to increase CS rate. Admission to maternity HDU, admission to ICU and mean Postoperative hospital stay (days) were significantly difference between women with placenta previa (PP) and placenta accreta (PA).
Journal of Current and Advance Medical Research, January 2022;9(1):36-41
Investigating Principals’ Leadership to Develop Teachers’ Professionalism at Madrasah
Authors: Warisno Andi , Hidayah Nur 
Published: May 2022

This study sought to explore the principals’ leadership in private Madrasah Aliyah schools on teachers’ professionalism in the learning process. It applied a quantitative approach with a survey method. Fifty-five teachers served as the samples, which were taken from two private schools in the South Lampung region. Data was collected through a questionnaire. Two instruments were constructed: the first is based on the indicators of leadership using talent management from Gallardo-Gallardo & Thunnissen, and the second is based on teacher competence. The r-test, with the assistance of SPSS 25, was applied to analyze the data. The results indicate an influence of the principals’ leadership on teacher competence. This is evidenced by the results of the r-test showing the value of sig. 0.000. This shows a significant relationship between the principals’ leadership and the teachers’ professionalism in Madrasah Aliyah. Leadership is one of the determining factors in improving teachers’ professionalism, school performance, and a conducive learning process.
Super-Enhancer-Associated Long Non-Coding RNA LINC01485 Promotes Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Regulating MiR-619-5p/RUNX2 Axis
Authors: Gu Wenli , Jiang Xiao , Wang Wei , Mujagond Prabhakar , Liu Jingpeng , Mai Zhaoyi , Tang Hai , li Simin , Xiao Hui , Zhao Jianjiang 
Published: May 2022

ObjectiveTo investigate the mechanisms of super-enhancer-associated LINC01485/miR-619-5p/RUNX2 signaling axis involvement in osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).MethodsOsteogenic differentiation of hBMSCs was induced in vitro. The expression levels of LINC01485 and miR-619-5p during osteogenesis were measured using quantitative real-time polymerase chain reaction (qRT-PCR). Osteogenic differentiation was examined by qRT-PCR, western blot, alkaline phosphatase (ALP) staining, ALP activity measurement, and Alizarin Red S (ARS) staining assays. Thereafter, the effects of LINC01485 and miR-619-5p on osteogenic differentiation of hBMSCs were evaluated by performing loss- and gain-of-function experiments. Subsequently, a fluorescence in situ hybridization (FISH) assay was employed to determine the cellular localization of LINC01485. Bioinformatics analysis, RNA antisense purification (RAP) assay, and dual-luciferase reporter assays were conducted to analyze the interactions of LINC01485, miR-619-5p, and RUNX2. Rescue experiments were performed to further delineate the role of the competitive endogenous RNA (ceRNA) signaling axis consisting of LINC01485/miR-619-5p/RUNX2 in osteogenic differentiation of hBMSCs.ResultsThe expression of LINC01485 was up-regulated during osteogenic differentiation of hBMSCs. The overexpression of LINC01485 promoted osteogenic differentiation of hBMSCs by up-regulating the expression of osteogenesis-related genes [e.g., runt-related transcription factor 2 (RUNX2), osterix (OSX), collagen type 1 alpha 1 (COL1A1), osteocalcin (OCN), and osteopontin (OPN)], and increasing the activity of ALP. ALP staining and ARS staining were also found to be increased upon overexpression of LINC01485. The opposing results were obtained upon LINC01485 interference in hBMSCs. miR-619-5p was found to inhibit osteogenic differentiation. FISH assay displayed that LINC01485 was mainly localized in the cytoplasm. RAP assay results showed that LINC01485 bound to miR-619-5p, and dual-luciferase reporter assay verified that LINC01485 bound to miR-619-5p, while miR-619-5p and RUNX2 bound to each other. Rescue experiments illustrated that LINC01485 could promote osteogenesis by increasing RUNX2 expression by sponging miR-619-5p.ConclusionLINC01485 could influence RUNX2 expression by acting as a ceRNA of miR-619-5p, thereby promoting osteogenic differentiation of hBMSCs. The LINC01485/miR-619-5p/RUNX2 axis might comprise a novel target in the bone tissue engineering field.
Expression of Functional Molecule on Plasmacytoid Dendritic Cells Is Associated With HBsAg Loss in HBeAg-Positive Patients During PEG-IFN α-2a Treatment
Authors: Cao Weihua , Xie Si , Zhang Lu , Bi Xiaoyue , Lin Yanjie , Yang Liu , Lu Yao , Liu Ruyu , Chang Min , Wu Shuling , Shen Ge , Dong Jianping , Xie Yao , Li Minghui 
Published: May 2022

ObjectiveThe ideal endpoint of antiviral therapy in chronic hepatitis B (CHB) patients is to clear hepatitis B surface antigen (HBsAg). This study aimed to evaluate whether the expression of functional molecules on plasmacytoid dendritic cells (pDCs) is associated with HBsAg loss in HBeAg-positive patients during peginterferon alpha-2a (PEG IFN α-2a) therapy.MethodsA single-center prospective cohort study was performed in HBeAg-positive CHB patients who were treated with PEG-IFN α-2a and followed up for 4 years. HBsAg clearance, HBeAg loss and undetectable HBV DNA achieved by PEG-IFN α-2a therapy was considered as functional cure. The frequencies of pDC and CD86+ pDC in peripheral blood, and the mean fluorescence intensity of CD86 (CD86MFI) on the surface of pDC were measured at starting therapy, after 12 and 24 weeks of therapy.ResultsOf 63 patients enrolled, 17 patients achieved HBsAg loss. The baseline HBV DNA load in Non-functional-cure group was significantly higher than that in Functional cure group, and the CD86+ pDC% was significantly lower in patients without functional cure. HBV DNA load (OR=0.146, P = 0.002) and CD86+ pDC% (OR=1.183, P = 0.025) were independent factors associated with functional cure confirmed by binary logistic regression analysis. In the Functional cure group, HBsAg, HBeAg, and HBV DNA loads decreased remarkably after 12 weeks and 24 weeks of treatment compared to baseline. In Non-functional-cure group, CD86+ pDC% and CD86MFI increased significantly from baseline after 12 weeks of treatment. In the Functional cure group, compared with baseline, pDC% increased significantly at 24 weeks, while CD86MFI increased significantly after 24 weeks of treatment.ConclusionThe lower the baseline HBV DNA load and the more the baseline CD86+ pDC%, the easier it is for patients to obtain functional cure.
Glutamate Chemical Exchange Saturation Transfer (GluCEST) Magnetic Resonance Imaging of Rat Brain With Acute Carbon Monoxide Poisoning
Authors: Xu Yuan , Zhuang Zerui , Zheng Hongyi , Shen Zhiwei , Gao Qilu , Lin Qihuan , Fan Rong , Luo Liangping , Zheng Wenbin 
Published: May 2022

ObjectivesTo evaluate the diagnostic and prognostic values of glutamate chemical exchange saturation transfer (GluCEST) magnetic resonance imaging as a quantitative method for pathogenetic research and clinical application of carbon monoxide (CO) poisoning-induced encephalopathy combined with the proton magnetic resonance spectroscopy (1H-MRS) and the related histopathological and behavioral changes.MethodsA total of 63 Sprague–Dawley rats were randomly divided into four groups. Group A (n = 12) was used for animal modeling verification; Group B (n = 15) was used for magnetic resonance molecular imaging, Group C (n = 15) was used for animal behavior experiments, and Group D (n = 21) was used for histopathological examination. All the above quantitative results were analyzed by statistics.ResultsThe peak value of carboxyhemoglobin saturation in the blood after modeling was 7.3-fold higher than before and lasted at least 2.5 h. The GluCEST values of the parietal lobe, hippocampus, and thalamus were significantly higher than the base values in CO poisoning rats (p &lt; 0.05) and the 1H-MRS showed significant differences in the parietal lobe and hippocampus. In the Morris water maze tests, the average latency and distance were significantly prolonged in poisoned rats (p &lt; 0.05), and the cumulative time was shorter and negatively correlated with GluCEST.ConclusionThe GluCEST imaging non-invasively reflects the changes of glutamate in the brain in vivo with higher sensitivity and spatial resolution than 1H-MRS. Our study implies that GluCEST imaging may be used as a new imaging method for providing a pathogenetic and prognostic assessment of CO-associated encephalopathy.
Cerebral Ischemic Events: An Overlooked Complication of Transthyretin Cardiac Amyloidosis in Afro-Caribbean Patients
Authors: Banydeen Rishika , Signate Aissatou , Tran Tuan-Huy , Monfort Astrid , Neviere Remi , Inamo Jocelyn 
Published: May 2022

AimThe link between transthyretin cardiac amyloidosis (CATTR), and cerebral ischemic events (CIE) has only been hinted at till now, impeding progress in patient management. We seek to evaluate the frequency and characteristics of CIE in Afro-Caribbean patients followed for CATTR at our institution.MethodsIn this single-center retrospective observational study, Afro-Caribbean patients followed for CATTR between July 2005 and October 2019 were included. Occurrence of CIE was investigated, and their cardioembolic origin determined. Analysis of patient characteristics was conducted according to CIE and CATTR profiles.ResultsOverall, 120 CATTR patients were included: 17 wild-type ATTR (14.2%), 73 ATTR-V122I (60.8%), and 22 ATTR-I107V (18.3%). Thirty-six patients (30.0%) presented with CIE, including three transient ischemic attacks and 33 permanent ischemic strokes (75.8% with a cardioembolic pattern). CIE was concomitant with CATTR diagnosis in 16 (16/36: 44.4%) patients, while 14 patients (14/36: 38.9 %) experienced CIE over a median CATTR follow-up of 2.0 years (min-max range: 0.8–4.4 years). CATTR-CIE patients presented with atrial fibrillation (66.7%), left atrial enlargement (77.8%), a CHA2DS2-VASc ≥ 3 (97.2%) and a high anticoagulant intake (75.0%). Multivariate analysis retained only a high CHA2DS2-VASc score as an independent predictor of CIE risk (Hazard Ratio [95% CI]: 12.03 [1.62–89.24]).ConclusionConcomitant CIE, and CATTR diagnosis, potentially carries a worse prognosis. A CHA2DS2-VASc score ≥3 seems to be a strong and independent predictive factor of CIE in CATTR patients. Further studies are needed to assess the efficacy and timeliness of anticoagulation in CATTR patients, independently of atrial fibrillation.
Neuronal Circuits Supporting Development of Visual Naming Revealed by Intracranial Coherence Modulations
Authors: Arya Ravindra , Ervin Brian , Buroker Jason , Greiner Hansel M. , Byars Anna W. , Rozhkov Leonid , Skoch Jesse , Horn Paul S. , Frink Clayton , Scholle Craig , Leach James L. , Mangano Francesco T. , Glauser Tracy A. , Holland Katherine D. 
Published: May 2022

BackgroundImprovement in visual naming abilities throughout the childhood and adolescence supports development of higher-order linguistic skills. We investigated neuronal circuits underlying improvement in the speed of visual naming with age, and age-related dynamics of these circuits.MethodsResponse times were electronically measured during an overt visual naming task in epilepsy patients undergoing stereo-EEG monitoring. Coherence modulations among pairs of neuroanatomic parcels were computed and analyzed for relationship with response time and age.ResultsDuring the overt visual naming task, mean response time (latency) significantly decreased from 4 to 23 years of age. Coherence modulations during visual naming showed that increased connectivity between certain brain regions, particularly that between left fusiform gyrus/left parahippocampal gyrus and left frontal operculum, is associated with improvement in naming speed. Also, decreased connectivity in other brain regions, particularly between left angular and supramarginal gyri, is associated with decreased mean response time. Further, coherence modulations between left frontal operculum and both left fusiform and left posterior cingulate gyri significantly increase, while that between left angular and supramarginal gyri significantly decrease, with age.ConclusionNaming speed continues to improve from pre-school years into young adulthood. This age-related improvement in efficiency of naming environmental objects occurs likely because of strengthened direct connectivity between semantic and phonological nodes, and elimination of intermediate higher-order cognitive steps.
Elevated Serum Lactate in Glioma Patients: Associated Factors
Authors: Sitter Beathe , Forsmark Annamaria , Solheim Ole 
Published: May 2022

IntroductionSerum lactate levels in brain cancer patients correlate with tumor malignancy grading, and serum lactate has been suggested as a potential biomarker and prognostic factor. The purpose of this study was to identify potential sources of elevated serum lactate in patients with brain gliomas by examining factors of importance for serum lactate production and clearance.MethodsIn this cross-sectional study, data were collected from 261 glioma patients who underwent surgery from March 2011 to June 2015. We recorded patient gender, age, blood serum measures of lactate, glucose, pH, hemoglobin and base excess, patient health status, medications, and tumor characteristics. Patients with elevated and normal serum lactate levels were compared, and we explored if there were correlations between the variables. The association of serum lactate with the measured variables was investigated by simple and multivariable linear regression models.Results and DiscussionPatients with elevated serum lactate had higher blood glucose, larger tumor volumes, and more tumor edema; more often needed pressor medication during surgery; and more often received corticosteroid treatment. The investigated variables were highly correlated. Multivariable linear regression indicated that gender, tumor volume, Charlson Comorbidity Index, hyperglycemia, and corticosteroid treatment were associated with serum lactate levels. Histopathology was not an independent factor. In conclusion, comorbidities, hyperglycemia, and presurgical corticosteroid treatment exhibited the strongest association with serum lactate in glioma patients.
Application of Diffusion Kurtosis Imaging in Evaluating Acute Xerostomia in Nasopharyngeal Carcinoma Treated With Induction Chemotherapy Plus Concurrent Chemoradiotherapy
Authors: Zhao Da-Wei , Fang Xue-Mei , Zhou Shu-Han , Luo Yan-Rong , Wei Jian , Liu Kun , Meng Ling-Ling , Liu Gang , Li Jin-Feng , Zang Xiao , Li Meng , Qiang Xiao-Fei , Jiang Hua-Yong , Fan Wen-jun , Zhang Xin-Xin , Ma Lin 
Published: May 2022

PurposeThe aim of this study was to identify the efficacy of diffusion kurtosis imaging (DKI) in tracking and monitoring the dynamic change of parotid glands (PGs), submandibular glands (SMGs), sublingual glands (SLGs), and acute xerostomia in nasopharyngeal carcinoma (NPC) patients treated with induction chemotherapy (IC) plus concurrent chemoradiotherapy (CCRT).MethodsThe prospective study recruited 42 participants treated with IC+CCRT. All patients underwent DKI scanning six times: before IC, before RT, in the middle of the RT course, immediately after RT, and 1 and 3 months post-RT. Mean diffusion coefficient (MD) and mean kurtosis (MK) of PG, SMG, SLG, saliva flow rate measured under resting (uSFR) and stimulated condition (sSFR), and xerostomia questionnaire (XQ) scores were recorded.ResultsAt each time point, sSFR was significantly higher than uSFR (p &lt; 0.05 for all). MD of the salivary glands and XQ scores increased over time while MK, uSFR, and sSFR decreased. After IC, the significant differences were detected in MD and MK of bilateral SMG and MK of the left SLG (p &lt; 0.05 for all), but not in MD and MK of PG, uSFR, sSFR, and XQ scores. After RT, sSFR at 1m-RT decreased significantly (p = 0.03) while no significant differences were detected in uSFR and XQ scores. Moderate-strong correlations were detected in ΔMD-PG-R%, ΔMK-PG-R%, ΔMD-PG-L%, ΔMK-PG-L%, ΔMD-SMG-R%, ΔMK-SMG-R%, ΔMD-SMG-L%, ΔMK-SMG-L%, and ΔMD-SLG-R%, with correlation coefficients (p &lt; 0.05 for all) ranging from 0.401 to 0.714. ΔuSFR% was correlated with ΔMD-SMG% (p = 0.01, r = −0.39), ΔMD-SLG% (p &lt; 0.001, r = −0.532), and ΔMK-SMG% (p &lt; 0.001, r = −0.493). ΔsSFR% correlated with ΔMD-PG% (p = 0.001, r = −0.509), ΔMD-SMG% (p = 0.015, r = −0.221), and ΔMK-PG% (p &lt; 0.001, r = 0.524). ΔXQ% was only correlated with ΔMK-PG% (p = 0.004, r = 0.433).ConclusionDKI is a promising tool for tracking and monitoring the acute damage of PG, SMG, and SLG induced by IC+CCRT in NPC patients.
Early Mini-Invasive Treatment of Persistent Cervical Dysplasia: Clinical Outcome and Psycho-Relational Impact
Authors: Plotti Francesco , Rossini Gianmarco , Ficarola Fernando , De Cicco Nardone Carlo , Montera Roberto , Guzzo Federica , Luvero Daniela , Fabris Silvia , Angioli Roberto , Terranova Corrado 
Published: May 2022

IntroductionAfter the diagnosis of L-SIL, 77. 3% had a persistent infection and anomalous Pap Test results. Many of these patients had highlighted psychological consequences such as anxiety, hypochondria, fear of cancer, and sexual problems. Several studies suggested that the clearance of HR-HPV infection could be accelerated by cervical excisional procedures, especially in L-SIL. In consideration of the psychological implications for HPV infection and related dysplasia in patients with CIN1 at PAP-smear and HR-HPV positivity at least for 6 months, we decided to plan a prospective study where we tried to anticipate excisional cervical using a minimally invasive treatment: thin loop electrosurgical excision procedure (t-LEEP). This study aims to analyze the clearance of HR-HPV after 6 and 12 months, clinical outcomes related to t-LEEP, and the psycho-relational impact at 12 months after t-LEEP.Materials and MethodsWe enrolled patients with the diagnosis of L-SIL at PAP-smear and HR-HPV positivity with a persistent CIN 1 (at least for 6 months), confirmed by cervical biopsy. All enrolled patients underwent t-LEEP. We followed prospectively and performed for all patients the HPV DNA test at 6 (T1) and 12 months (T2) and STAI-Y and FSFI scores at T0 and T2.ResultsWe prospectively enrolled 158 patients, 22 are excluded for the established criteria. Patients with HR-HPV and CIN 1 lesions treated with t-LEEP had an overall clearance of 83.8% at T2. In subgroups analysis at T2, we had a regression: in smoker 71.8%, in contraceptive users 69.5%, in patients aged &lt;25 years 100%, aged 25–30 years 85%, aged 30–35 years 94.4%, aged 35–40 years 92%, and aged ≥40 years 89.1%, in HPV-16 96.4%, in HPV-53 89.5%, in HPV-18 87.5%, in HPV-31 86.6%, and in coinfected 3.5%. STAI-Y and FSFI after t-LEEP (T2) were statistically significant, reducing anxiety status and improving sexual function.ConclusionBased on these results, the use of t-LEEP in patients with persistent CIN 1 and HPV-HR at least for 6 months could be useful for accelerating HPV-HR clearance, in particular, for a subpopulation patient with an increased risk of progression and/or patients with psychological and sexual consequences of carrying an HR-HPV infection.