bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation of multifunctional biohydrogel sensors with one freeze–thaw process
Authors: Tian Yi , Zhu Xing , Gan Ting , He Bin , Wang Xuechuan 
Published: April 2022

AbstractConductive hydrogels have a significant prospect in the design of bioactuators, healthcare monitoring sensors, and flexible wearable devices. However, their poor temperature tolerance and biotoxicity limit their further applications. In this study, a biosensor based on hydrogels with good biocompatibility, antifreezing, and moisturizing properties was synthesized using a one freeze–thaw process. The sensor could withstand 60°C for 400 min or −20°C without freezing and maintain good conductivity. The excellent antibacterial activity and biocompatibility of the sensor ensure the safety of its biosecurity applications. Thus, the proposed biosensor has potential bio‐applications as a bionic material under extreme conditions.
Catalytic Hydrogenation of Epoxides to Alcohols
Authors: Thiyagarajan Subramanian , Gunanathan Chidambaram 
Published: April 2022

AbstractAtom‐economical catalytic reactions are a highly enticing strategy because all atoms of the starting materials are incorporated into the products. Catalytic hydrogenation of epoxides to alcohols is an attractive and alternative protocol to other synthetic methodologies for the synthesis of alcohols from alkenes. In the last two decades, catalytic hydrogenation of epoxides to alcohols has made remarkable progress in chemical synthesis. In this review, an overview of the catalytic hydrogenation of both terminal and internal epoxides to the corresponding alcohols is presented. An outline of both homogeneous and heterogeneous hydrogenation of epoxides to the corresponding alcohols is provided. Moreover, the selectivity, efficiency, and the reaction mechanisms of these epoxide hydrogenation reactions are highlighted.
Multimodal Imaging and Synergetic Chemodynamic/Photodynamic Therapy Achieved Using an NaGdF4,Yb,Er@ NaGdF4,Yb,Tm@NaYF4@Fe‐MOFs Nanocomposite
Authors: Ling Bo , Wang Yaguang , Mi Ruo , Wang Di , Chen Hongqi , Li Xiaohu , Zhang Ye , Wang Lun 
Published: April 2022

AbstractHere, NaGdF4,Yb,Er@NaGdF4,Yb,Tm@NaYF4 core@shell@shell three‐layer structure of upconversion nanoparticles (UCNPs) coated with Fe−Tetrakis (4‐carboxyphenyl) porphine (TCPP) metal‐organic frameworks (Fe‐MOFs) nanocomposite (UCNPs@MOFs) was designed and constructed for multimodal imaging and synergetic chemodynamic therapy (CDT)/photodynamic therapy (PDT) of tumors. The UCNPs@MOFs were successfully applied for tumor cells imaging in vitro and in vivo in near‐infrared (NIR) region. The doped Gd was used as contrast agent for the magnetic resonance imaging (MRI) of mouse tumors. The luminescence in the UV‐vis region was absorbed by the Fe‐MOFs to produce singlet oxygen (1O2) for PDT. The Fe3+ doped in the MOFs can catalyze H2O2 to produce oxygen and hydroxyl radical (⋅OH). Hydroxyl radical is used in CDT and cooperates with the 1O2 of PDT. Based on the CDT/PDT synergistic effects, the UCNPs@MOFs nanocomposite had obviously enhanced tumor inhibitory efficiency in vivo. These results described that the asprared UCNPs@MOFs nanocomposite have great potential in the effective multimodal imaging and treatment of tumors.
Recent Advances in Fluorescent Chemosensors for Protein Kinases
Authors: Hu Jun , Li Gao 
Published: April 2022

AbstractProtein kinases are involved in almost all biological activities. The activities of different kinases reflect the normal or abnormal status of the human body. Therefore, detecting the activities of different kinases is important for disease diagnosis and drug discovery. Fluorescent probes offer opportunities for studying kinase behaviors at different times and spatial locations. In this review, we summarize different kinds of fluorescent chemosensors that have been used to detect the activities of many different kinases.
exo′‐Selective Construction of Spirobipyrrolidines by the Silver‐catalyzed Asymmetric [3+2] Cycloaddition of Imino Esters with 4‐Benzylidene‐2,3‐dioxopyrrolidines
Authors: Furuya Shohei , Kanemoto Kazuya , Fukuzawa Shin‐ichi 
Published: April 2022

AbstractAn unprecedented Ag‐catalyzed exo′‐selective [3+2] cycloaddition of imino esters with 4‐benzylidene‐2,3‐dioxopyrrolidines is described. The reaction was efficiently catalyzed by Ag/(R, Sp)‐ThioClickFerrophos (TCF) leading to the construction of the corresponding spirobipyrrolidine scaffolds in excellent enantio‐ and diastereoselectivities. This reaction is the first example of a silver‐catalyzed exo′‐selective asymmetric [3+2] cycloaddition, as well as the first exo′‐selective spirobipyrrolidine construction via a [3+2] cycloaddition process using imino esters. The wide substrate scope of this reaction enabled the preparation of structurally diverse spirobipyrrolidine derivatives, which are attracting attention as targets for drug discovery. Mechanistic studies suggested that the unusual exo′‐selectivity of this reaction is not due to epimerization following the common exo‐ or endo‐selective cycloaddition, but instead is due to a stepwise Michael addition/Mannich sequence with bond rotation.
The Sulfinylsulfonation of alkynes for β‐Sulfinyl alkenylsulfone
Authors: Zhang Zhansong , Song Qingmin , Feng Chengjie , Wang Zikun , Zhao Wanjun , Ning Yongquan , Wu Yong 
Published: April 2022

AbstractHere we report the sulfinylsulfonation of alkynes to afford β‐sulfinyl alkenylsulfone products with a broad substrate scope, excellent functional group compatibility, and high yield. Moreover, the sulfinylsulfonation reaction of enyne can also be realized for constructing functionalized carbo‐ and heterocycles through a radical cascade cyclization process.
Brønsted Acid‐Catalysed Epoxide Ring‐Opening Using Amine Nucleophiles: A Facile Access to β‐Amino Alcohols
Authors: Tyagi Aparna , Yadav Naveen , Khan Jabir , Mondal Sankalan , Hazra Chinmoy Kumar 
Published: April 2022

AbstractA mild, efficient, and metal‐free synthetic protocol for the synthesis of β‐amino alcohols is reported. The reaction proceeds at room temperature with only 0.5 mol % catalyst loading and affords β‐amino alcohol derivatives in excellent yield. This protocol is well‐tolerated by a wide range of styrene oxide and aniline derivatives. A notably efficacious gram‐scale synthesis is also reported with a high TON=842. Further, the Hammett correlation study was also performed to identify the rate‐determining step.
Review of electrochemical and optical biosensors for testosterone measurement
Authors: Nikkhah Maryam , Karami Sajedeh , Khatami Seyyed Hossein , Taheri‐Anganeh Mortaza , Savardashtaki Amir , Mahmoodzadeh Amir , Shabaninejad Zahra , Vakili Omid , Mousavi Pegah , Ghanizadeh Gerayeli Farhad , Behrouj Hamid , Ghasemi Hassan , Movahedpour Ahmad 
Published: April 2022

AbstractTestosterone is an anabolic steroid and a major sex hormone in males. It plays vital roles, including developing the testis, penis, and prostate, increasing muscle and bone, and sperm production. In both men and women, testosterone levels should be in normal ranges. Besides, testosterone and its analogs are major global contributors to doping in sport. Due to the importance of testosterone testing, novel, accurate biosensors have been developed. This review summarizes the various methods for testosterone measurement. Also, recent optical and electrochemical approaches for the detection of testosterone and its analogs have been discussed.
Gestational purple grape juice consumption does not change fetal ductus arteriosus constriction in Wistar rats
Authors: Proença Isabel Cristina Teixeira , Miri Malena Rostirola , Marinho Jéssica Pereira , Bock Patrícia Martins , de Abreu Tamires Marques , Pinto Milene Santana , Blembeel Amanda Stolzenberg , Ferreira Aimée Souto , Andrades Michael , da Rosa Araújo Alex Sander , Funchal Cláudia , Pochmann Daniela , Dani Caroline 
Published: April 2022

AbstractGrape juice consumption may influence the early occurrence of ductal constriction during pregnancy, since the consumption of foods rich in polyphenols can be linked to the premature constriction of the ductus arteriosus. This study aimed to evaluate the effect of purple grape juice consumption during gestation on fetal ductus arteriosus closure, prostaglandin levels, and oxidative stress markers in Wistar rats. We divided 18 pregnant rats into four groups: a control group (C), a single‐dose grape juice group (SDGJ), a two‐dose grape juice group (TDGJ) of 7 μl/g body weight per day, and an indomethacin group (I). Blood was collected on gestational day (GD) 0, 14, and 20. Prostaglandin levels were measured, and the livers and hearts were removed from the mothers and fetuses for oxidative stress analysis; histology of the fetal ductus arteriosus was performed. Prostaglandin levels (pg/ml) at GD 20 were (C:1462.10 ± 314.61); (SDGJ:987.66 ± 86.25); (TDGJ:1290.00 ± 221.57), and (I:584.75 ± 46.77). Fetal ductus arteriosus closure occurred only in the indomethacin group. Lipid peroxidation evaluated through thiobarbituric acid reactive substances (nmol/mg protein) in maternal livers was lower in the grape juice groups (C: 4.11 ± 0.76 nmol/mg protein), (SDGJ: 2.34 ± 0.36), (TDGJ: 1.52 ± 0.18), and (I: 4.20 ± 0.76). Sulfhydryls (nmol/mg protein) were lower in the TDGJ group (C:763.59 ± 61.38 nmol/mg protein), (SDGJ:978.88 ± 158.81), (TDGJ:385.32 ± 86.78), and (I:727.72 ± 49.12). Also, superoxide dismutase activity (USOD/mg protein) was higher in fetal hearts in this group: (C:5.29 ± 0.33), (SDGJ:4.48 ± 0.47), (TDGJ:7.35 ± 0.43), and (I:6.00 ± 0.18). We conclude that grape juice consumption in pregnancy does not induce ductus arteriosus closure in the fetus and presented potential antioxidant effects.
Mammalian D‐cysteine: A novel regulator of neural progenitor cell proliferation
Authors: Roychaudhuri Robin , Snyder Solomon H. 
Published: April 2022

AbstractD‐amino acids are being recognized as functionally important molecules in mammals. We recently identified endogenous D‐cysteine in mammalian brain. D‐cysteine is present in neonatal brain in substantial amounts (mM) and decreases with postnatal development. D‐cysteine binds to MARCKS and a host of proteins implicated in cell division and neurodevelopmental disorders. D‐cysteine decreases phosphorylation of MARCKS in neural progenitor cells (NPCs) affecting its translocation. D‐cysteine controls NPC proliferation by inhibiting AKT signaling. Exogenous D‐cysteine inhibits AKT phosphorylation at Thr 308 and Ser 473 in NPCs. D‐cysteine treatment of NPCs led to 50% reduction in phosphorylation of Foxo1 at Ser 256 and Foxo3a at Ser 253. We hypothesize that in the developing brain endogenous D‐cysteine is as a physiologic regulator of NPC proliferation by inhibiting AKT signaling mediated by Foxo1 and Foxo3a. Endogenous D‐cysteine may regulate mammalian neurodevelopment with roles in schizophrenia and Alzheimer's disease (AD).
An encapsulated fibrin‐based bioartificial tissue construct with integrated macrovessels, microchannels, and capillary tubes
Authors: Helms Florian , Zippusch Sarah , Theilen Jonathan , Haverich Axel , Wilhelmi Mathias , Böer Ulrike 
Published: April 2022

AbstractFacilitating sufficient nutrient and oxygen supply in large‐scale bioartificial constructs is a critical step in organ bioengineering. Immediate perfusion not only depends on a dense capillary network, but also requires integrated large‐diameter vessels that allow vascular anastomoses during implantation. These requirements set high demands for matrix generation as well as for in vitro cultivation techniques and remain mostly unsolved challenges up until today. Additionally, bioartificial constructs must have sufficient biomechanical stability to withstand mechanical stresses during and after implantation. We developed a bioartificial tissue construct with a fibrin matrix containing human umbilical vein endothelial cells and adipose tissue‐derived stem cells facilitating capillary‐like network formation. This core matrix was surrounded by a dense acellular fibrin capsule providing biomechanical stability. Two fibrin‐based macrovessels were integrated on each side of the construct and interconnected via four 1.2 mm thick microchannels penetrating the cellularized core matrix. After 4 days of perfusion in a custom‐built bioreactor, homogeneous capillary‐like network formation throughout the core matrix was observed. The fibrin capsule stabilized the core matrix and facilitated the generation of a self‐supporting construct. Thus, the encapsulated fibrin tissue construct could provide a universal prevascularized matrix for seeding with different cell types in various tissue engineering approaches.
A digital intervention using virtual reality helmets to reduce dental anxiety of children under local anesthesia and primary teeth extraction: A randomized clinical trial
Authors: Du Qin , Ma Xinru , Wang Shasha , Zhou Shiyu , Luo Chunmei , Tian Kun , Fei Wei , Liu Xianghong 
Published: April 2022

AbstractIntroductionBehavior management of children during dental treatment is an important but challenging issue. As a new technique, VR has been applied in pediatric dental anxiety. But there is no final conclusion whether VR reduces children's dental anxiety.MethodsThe aim of the study is to assess the effectiveness of a digital intervention using virtual reality (VR) helmets on dental anxiety, pain perception, and behavior triggered for children, as well as occurrence of simulator sickness in local anesthesia and primary teeth extraction. A total of 128 children, who needed primary teeth extraction under local anesthesia, were randomly allocated into two groups: use VR helmets and traditional behavior guidance procedures (control). Modified Child Fear Survey Schedule Dental Subscale (CFSS‐DS), Wong‐Baker FACES Pain Scale, Houpt Scale, and Simulator sickness questionnaire (SSQ) were used to assess children's dental anxiety, pain perception, and behavior triggered and occurrence of simulator sickness.ResultsCFSS‐DS score in the VR group was significantly decreased after dental treatment (34.58±6.90 before operation and 32.32±15.58 after operation, p = .02). The score of Wong Baker Scale in the VR group (3.47±0.76) was significantly lower than that in the control group (5.56±1.13, p = .015). There was no significant difference in the Houpt Behavior Scale score and the SSQ score between the VR group and the control group (p = .35, p = .305).ConclusionThe use of VR helmets in primary teeth extraction can significantly reduce dental anxiety and pain perception in children without occurrence of simulator sickness.
Assessment of the texture and digestion properties of a high amylose content rice cultivar under various water‐to‐rice ratios
Authors: Hu Liqin , Cao Jialin , Zhang Mingyu , Liu Yu , Xiao Zhengwu , Iqbal Anas , Huang Min 
Published: April 2022

AbstractBackground and ObjectivesIncreasing the water‐to‐rice (W/R) ratio can alleviate the hardness of cooked rice with high amylose content. However, whether it affects starch digestibility remains unknown. To determine the effect of the W/R ratio during cooking on starch digestion and texture properties, three W/R ratios, that is, 1.2, 1.6, and 2.0, two cultivars with different amylose contents: Zhenguiai (ZGA, 26.8 g/100 g) and Yuzhenxiang (YZX, 17.2 g/100 g) were evaluated.FindingsOur results showed that the different W/R ratios had no significant effect on the glucose production rate (GPR) of the ZGA and YZX cultivars. However, when the W/R ratio increased from 1.2 to 2.0, the hardness and chewiness of ZGA decreased by 33.5% and 42.0%, respectively. The hardness and chewiness of ZGA were quite close to YZX under the 2.0 W/R ratio. In addition, the GPR of ZGA was 48% lower than that of YZX under the same cooking condition.ConclusionsThe hard texture of the high amylose content cultivar can be maximally adjusted without affecting digestion properties by increasing the W/R ratio in the range of 1.2–2.0.Significance and NoveltyDiabetic patients will not have to endure the hard texture of high‐amylose rice. Rice producers can recommend more than one W/R ratio for consumers.
Binding of VIVO2+, VIVOL, VIVOL2 and VVO2L Moieties to Proteins: X‐ray/Theoretical Characterization and Biological Implications
Authors: Santos Marino F. A. , Sciortino Giuseppe , Correia Isabel , Fernandes Andreia C. P. , Santos‐Silva Teresa , Pisanu Federico , Garribba Eugenio , Costa Pessoa João 
Published: April 2022

AbstractVanadium compounds have frequently been proposed as therapeutics, but their application has been hampered by the lack of information on the different V‐containing species that may form and how these interact with blood and cell proteins, and with enzymes. Herein, we report several resolved crystal structures of lysozyme with bound VIVO2+ and VIVOL2+, where L=2,2’‐bipyridine or 1,10‐phenanthroline (phen), and of trypsin with VIVO(picolinato)2 and VVO2(phen)+ moieties. Computational studies complete the refinement and shed light on the relevant role of hydrophobic interactions, hydrogen bonds, and microsolvation in stabilizating the structure. Noteworthy is that the trypsin−VVO2(phen) and trypsin−VIVO(OH)(phen) adducts correspond to similar energies, thus suggesting a possible interconversion under physiological/biological conditions. The obtained data support the relevance of hydrolysis of VIV and VV complexes in the several types of binding established with proteins and the formation of different adducts that might contribute to their pharmacological action, and significantly widen our knowledge of vanadium–protein interactions.
Evaluation of Pharmacokinetics and Safety With Bioequivalence of Anastrozole in Healthy Chinese Volunteers: Bioequivalence Study Findings
Authors: Wang Ying , Wang Tongtong , Fang Han , Huang Hui , Fang Lijuan , Zhang XuanGuo 
Published: April 2022

AbstractAnastrozole is a third‐generation aromatase inhibitor that exerts potent anti–breast cancer effects. This trial aimed to explore the pharmacokinetics (PK) and safety with bioequivalence of orally administered anastrozole provided by 2 sponsors in healthy volunteers.Two separate open‐label, randomized, single‐dose, crossover‐design studies consisting of a fasting study (n = 23) and a fed study (n = 23, 1 participant withdrew before taking medication) were conducted. In each study, healthy volunteers were randomized to receive the test product (Haizheng Pharmaceutical Group) followed by the reference drug (AstraZeneca Pharmaceuticals LP), or vice versa. Each study subject received a 1‐mg anastrozole tablet with a 21‐day washout. The plasma concentrations of anastrozole were measured with liquid chromatography–tandem mass spectrometry, and PK parameters were determined by noncompartmental analysis. Forty‐six healthy female volunteers were enrolled. For patients enrolled in the fasting study, the mean age was 55.0 years, mean weight was 57.1 kg, mean body mass index was 23.6 kg/m2, and mean height was 155.5 cm. For patients enrolled in the fed study, the mean age was 54.2 years, mean weight was 55.9 kg, mean body mass index was 23.9 kg/m2, and mean height was 152.8 cm. All PK end points met the predefined criteria for PK equivalence. In fasting subjects, the median maximum plasma concentration was 23.4 and 22.6 at 1 hour for test and reference formulations, respectively. The maximum plasma concentration in fed subjects was 18.7 and 18.5 at 4 hours for test and reference formulations, respectively. Both fasting and fed studies achieved plausible bioequivalence. Anastrozole was well tolerated and exhibited a favorable safety profile at the prescribed doses. The severity of observed adverse events assessed according to the Common Terminology Criteria for Adverse Events (version CTCAE4.03) was mild, and some of the adverse events were not caused by anastrozole. Furthermore, the results of our study under fasting and fed conditions demonstrated bioequivalence of the test and reference products.
Contextualized Drug–Drug Interaction Management Improves Clinical Utility Compared With Basic Drug–Drug Interaction Management in Hospitalized Patients
Authors: Wasylewicz Arthur T.M. , van de Burgt Britt W.M. , Manten Thomas , Kerskes Marieke , Compagner Wilma N. , Korsten Erik H.M. , Egberts Toine C.G. , Grouls Rene J.E. 
Published: April 2022

Drug–drug interactions (DDIs) frequently trigger adverse drug events or reduced efficacy. Most DDI alerts, however, are overridden because of irrelevance for the specific patient. Basic DDI clinical decision support (CDS) systems offer limited possibilities for decreasing the number of irrelevant DDI alerts without missing relevant ones. Computerized decision tree rules were designed to context‐dependently suppress irrelevant DDI alerts. A crossover study was performed to compare the clinical utility of contextualized and basic DDI management in hospitalized patients. First, a basic DDI‐CDS system was used in clinical practice while contextualized DDI alerts were collected in the background. Next, this process was reversed. All medication orders (MOs) from hospitalized patients with at least one DDI alert were included. The following outcome measures were used to assess clinical utility: positive predictive value (PPV), negative predictive value (NPV), number of pharmacy interventions (PIs)/1,000 MOs, and the median time spent on DDI management/1,000 MOs. During the basic DDI management phase 1,919 MOs/day were included, triggering 220 DDI alerts/1,000 MOs; showing 57 basic DDI alerts/1,000 MOs to pharmacy staff; PPV was 2.8% with 1.6 PIs/1,000 MOs costing 37.2 minutes/1,000 MOs. No DDIs were missed by the contextualized CDS system (NPV 100%). During the contextualized DDI management phase 1,853 MOs/day were included, triggering 244 basic DDI alerts/1,000 MOs, showing 9.6 contextualized DDIs/1,000 MOs to pharmacy staff; PPV was 41.4% (P < 0.01), with 4.0 PIs/1,000 MOs (P < 0.01) and 13.7 minutes/1,000 MOs. The clinical utility of contextualized DDI management exceeds that of basic DDI management.
Isolated seven‐level inverter for grid‐connected power systems with predictive control method
Authors: Ortatepe Zafer 
Published: April 2022

AbstractA novel isolated 7‐level (7L) multi‐level inverter (MLI) topology for grid‐connected medium and high voltage power applications is presented in this paper. The topology consists of two isolated transformers with different winding ratio and four switches connected to a single‐phase grid. This topology provides a great advantage over conventional MLIs by reducing passive and active components to allowing a 7L output voltage. Moreover, model predictive control (MPC) method is implemented to 7L‐MLI to generate gate signals of switches and ensure capacitor voltage balancing and grid connection. Performance of proposed topology has been confirmed by in‐depth analysis under simulation and laboratory setup with existing studies. Experimental results of a 1.5 kW 220 Vrms prototype are presented to support the simulation results. The prototype shows that the harmonic results match the limits of the IEC 61000‐3‐2 standard and show a better performance for grid connection and capacitor voltage balancing than conventional ones.