bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased Endogenous PKG I Activity Attenuates EGF-Induced Proliferation and Migration of Epithelial Ovarian Cancer Via the MAPK/ERK Pathway
Authors: Lan Ting , Li Ying , Wang Yue , Wang Zhongcheng , Mu Chunyan , Tao Aibin , Gong Jianli , Zhou Yuan , Xu Hao , Li Shibao , Gu Bing , Ma Ping , Luo Lan 
Published: March 2022

Abstract
Background
The type I cGMP-dependent protein kinase (PKG I) is recognized as a tumor suppressor, but its role in EGFR regulated epithelial ovarian cancer (EOC) progression remains unclear.
Methods
We evaluated the in vivo and in vitro effects of activated PKG I in EGF-induced EOC cell proliferation, migration, and invasion.
Results
The expressions of EGFR and PKG I were elevated, but the activated PKG I was decreased in EOC tissues of patients and cells lines. The addition of 8-Br-cGMP, a specific PKG I activator, attenuated the EGF-induced EOC cell proliferation, migration, and invasion in vitro. Similarly, activated PKG I also attenuated EOC progression in vivo using an EOC xenograft nude mouse model. The activated PKG I interacted with EGFR, causing increased threonine (693) phosphorylation and decreased tyrosine (1068) phosphorylation of EGFR, which resulted in disrupted EGFR-SOS1-Grb2 combination. Subsequently, the cytoplasmic phosphorylation of downstream proteins (c-Raf, MEK1/2, and ERK1/2) were declined, impeding the phosphorylated ERK1/2's nucleus translocation, and this reduction of phosphorylated tyrosine (1068) EGFR and ERK1/2 were also abolished by Rp-8-Br-cGMPS.
Conclusions
Our results suggest that the activation of PKG I attenuates EGF-induced EOC progression, and the 8-Br-cGMP-PKG I-EGFR/MEK/ERK axis might be a potential target for EOC therapy.
Recurrent aortic-arch pseudoaneurysm eroding into the left main bronchus following descending thoracic aortic stent repair
Authors: Duan Lijun , Pan Ruonan , Lu Xiaoqian , Cao Dianbo 
Published: March 2022

Abstract
Background: Behcet's disease (BD) is a form of systematic vasculitis that affects vessels of various sizes anywhere in the body. Aortic pseudoaneurysm is the most hazardous lesion in Behcet's disease (BD) and is associated with high mortality rates once rupture. Case presentation: In this report, we present a patient with known history of Behcet's disease (BD), whose recurrent aortic-arch pseudoaneurysm eroding into the left main bronchus was identified after a 4-year duration of endovascular aortic repair (EVAR) for thoracic descending aortic pseudoaneurysm ruptured into the left lung. Repeated endovascular aortic repair (EVAR) combined with the chimney stent effectively controlled massive haemoptysis under life-threatening conditions, and the patient was good condition at the 5-year follow-up. Conclusions: Endovascular aortic repair (EVAR) can be used as an effective and problem-solving treatment approach for thoracic aortic aneurysms (TAA) eroding into the lung, even recurrent pseudoaneurysm after endovascular aortic repair (EVAR) in BD patients. Among the imaging techniques assessing the technical success, outcome and complications, CT angiography (CTA) offers a fast, accessible and sensitive imaging modality.
Waveguide-integrated PdSe2 photodetector with high responsivity and speed in the near-infrared range across the O+E+S+C bands
Authors: Wu Jianghong , Ma Hui , Zhong Chuyu , Wei Maoliang , Sun Chunlei , Ye Yuting , Xu Yan , Tang Bo , Luo Ye , Sun Boshu , Jian Jialing , Dai Hao , Lin Hongtao , Li Lan 
Published: March 2022

Abstract
Hybrid integration of two-dimensional (2D) materials on a silicon photonic platform enables diverse exploration of novel active functions and significant improvement in device performance for next-generation silicon integrated photonic circuits. 2D transition metal dichalcogenide materials (TMDCs) have attracted extensive attention for photodetectors, but developing high-performance waveguide-integrated photodetectors based on conventionally investigated 2D TMDCs at the telecom C-band is still a big challenge because of their large optical bandgap and slow carrier mobility. Here, we propose PdSe2, a new type of 2D TMDC, to be integrated with silicon photonic components for on-chip photodetection with high responsivity and speed in the datacom and telecom optical bands ranging from the O-band to the C-band. The obtained waveguide-integrated photodetectors show a high responsivity of 1190.2 mA W−1 at 1550 nm and a low noise-equivalent power of 4.0 pW Hz-0.5 at 5V. The 3 dB bandwidth reaches up to 1.5 GHz, and the measured data rate is 2.5 Gbit s−1. The achieved PdSe2 photodetectors provide new insights to explore the integration of novel 2D TMDCs with silicon photonics and demonstrate the potential of integrated photodetectors for applications in diverse areas, including optical communications, on-chip spectroscopy, and sensing.
Clinical analysis of osteoporotic vertebral oblique compression fracture treated with bone cement combined with balloon
Authors: Deng Lei , Hu Xia-Yu , Hua Xi , Pan Ze-Jun , Zhou Quan , Li Bao-Xin , Lv Nan-Ning , Qian zhong-lai 
Published: March 2022

Abstract
ObjectiveTo explore the clinical effect of bone cement combined with balloon expansion in the treatment of osteoporotic vertebral oblique compression fracture. MethodsThis study retrospectively reviewed the patients who were diagnosed with osteoporotic vertebral oblique compression fracture between January 2017 and July 2019. According to the method of surgery, 41 patients received bone cement treatment alone (group A) and 44 patients received bone cement combined with balloon expansion (group B). The visual analogue scale (VAS), oswestry disability index (ODI), scoliosis angle (SA), height of long side (HL), height of short side (HS) and lateral height difference (LHD) before operation and 3 days and 1 year after operation were compared between the two groups. The operation time, fluoroscopic time, hospital stay, cement volume and complications were also compared between the two groups. ResultsThe VAS and ODI differed significantly between the groups 1 year after operation(P＜ 0.05). Compared with the preoperative results, there were significant differences in SA, HL, HS and LHD 3 days and 1 year after surgery(P＜0.05). There were significant differences in SA, HS and LHD 3 days and 1 year after surgery between the two groups(P＜0.05). A total of 21 patients in group A and 6 patients in group B experienced scoliosis after operation, there were also significant differences between groups(P＜0.05). ConclusionsBone cement combined with balloon expansion and bone cement alone are all effective in the treatment of osteoporotic vertebral oblique compression fracture, but bone cement combined with balloon expansion had better long-term clinical efficacy.
Diallel analysis using graphical &amp; numerical approach for grain yield &amp; its attributes in bread wheat (Triticum aestivum L.)
Authors: Chaudhari Gita R. , Patel D. A. , Kalola A. D. , Kumar Sushil 
Published: March 2022

Abstract
Ten wheat (Triticum aestivum L.) genotypes are crossed in half diallel fashion. Components of gene effect revealed that most of the characters were governed by both additive and dominance gene effect across the environments with greater influence of dominance gene effect except for days to 50% heading, days to maturity and grain yield per main spike. The estimated ‘t2 ’ values were found significant for all the character except days to 50% heading, which indicates the failure of assumptions of diallel analysis. The over dominance behaviour was observed for days to 50% heading, days to maturity, no. of effective tillers per plant , length of main spike, no. of spikelets per main spike, no. of grains per main spike, grain yield per main spike and 1000 grain weight. The array points on the Wr-Vr graph expected to fall on the line of unity (450) and the value of regression “b” was unity for days to 50% heading and 1000 grain weight which revealed an absence of digenic interactions for these characters and rest of the characters had non-random distribution of genes at different loci among the parents and/ or presence of inter allelic interaction at different loci.
Alcam-a and Pdgfr-α are essential for the development of sclerotome derived stromal cells that support hematopoiesis in vivo.
Authors: Murayama Emi , Vivier Catherine , Schmidt Anne , Touret Anne-Lou , Herbomel Philippe 
Published: March 2022

Abstract
Mesenchymal stromal cells are essential components of hematopoietic stem and progenitor cell (HSPC) niches, regulating HSPC proliferation and fate decisions. Their developmental origins are largely unknown. In zebrafish, we previously found that the stromal cells of the caudal hematopoietic tissue (CHT), a niche functionally homologous to the fetal liver in mammals, arise from the ventral part of caudal somites. We have now discovered that this ventral domain is actually the sclerotome, and that two typical markers of mammalian mesenchymal stem/stromal cells, Alcam and Pdgfr-α, are distinctively expressed there and instrumental for the emergence and migration of stromal cell progenitors, which in turn conditions the proper assembly of the vascular component of the CHT niche. Furthermore, we find that the trunk somites are similarly dependent on Alcam and Pdgfr-α to produce mesenchymal stromal cells that foster the initial emergence of HSPCs from the dorsal aorta. Thus the sclerotome contributes essential stromal cells for each of the key steps of developmental hematopoiesis, and likely is the embryological origin of most if not all mesenchymal stem/stromal cells found in non-cephalic tissues.
Bots influence opinion dynamics without direct human-bot interaction: the mediating role of recommender systems
Authors: Pescetelli Niccolo , Barkoczi Daniel , Cebrian Manuel 
Published: March 2022

Abstract
Bots’ ability to influence public discourse is difficult to estimate. Recent studies found that hyperpartisan bots are unlikely to influence public opinion because bots often interact with already highly polarized users. However, previous studies focused on direct human-bot interactions (e.g., retweets, at-mentions, and likes). The present study suggests that political bots, zealots, and trolls may affect people’s views indirectly via the mediating role of a platform’s content recommendation system, thus influencing opinions even in the absence of direct human-bot interaction. Using an agent-based opinion dynamics simulation, we isolated the effect of a single bot – representing 1% of nodes in a network – on the opinion formed by rational Bayesian agents after removing direct human-bot connections. We compare this experimental condition with an identical baseline condition where such a bot is absent. We used the same random seed in both simulations so that conditions remained identical except for the presence of the bot. Results show that, even in the absence of direct interactions, the mere presence of the bot is sufficient to shift the average population opinion. Virtually all nodes – not only nodes directly interacting with the bot – shifted towards more extreme opinions. Overall, these findings offer a proof of concept that bots and hyperpartisan accounts can influence population opinions not only by directly interacting with humans but also by secondary effects, such as shifting platforms’ recommendation engines’ internal representations. The mediating role of recommender systems creates indirect causal pathways of algorithmic opinion manipulation.
Investigation of the Cooperative Mode and Second Language Acquisition of Chinese Apology Words from the Perspective of Construction Grammar
Authors: Lun Xinyu 
Published: March 2022

Abstract
Based on the theory of construction grammar, this paper examines the cooperative mode and second language acquisition of Chinese apology words. It demonstrates the acquisition process and mechanism of Chinese apology words by second language learners. From the perspective of construction grammar, Chinese apology words are the intersection of form and meaning/function. There are frame structures and non-frame structures in the cooperative mode, and additionally, there are complete and omitted forms. The learner's output of apology words includes errors and correct usage. Errors include wrong coordinated mode selection, out-of-order coordination components, incomplete coordination components, and misuse of coordination components. The correct output refers to the cooperative model of the learner's apology words, including the location, usage, and selection of the components, which can be used accurately. The internal acquisition mechanism of these outputs is the learner's "coordination awareness" of apology words. The mastery of "coordination awareness" can be divided into levels and stages. There are three levels in StageⅠ, namely, no coordination awareness, preliminary coordination awareness and basic coordination awareness. StageII means that the learner has complete coordination awareness, which shows that the learner's acquisition of apology words is staged and hierarchical.
Culture Negative Sepsis after Pediatric Cardiac Surgery: Incidence and Outcomes
Authors: Gopalakrishnan Rajesh M , Nair Arjun R , Sudhakar Abish , Jayant Aveek , Balachandran Rakhi , Neema Praveen Kumar , Kumar Raman Krishna 
Published: March 2022

Abstract
Background: A significant proportion of children after cardiac surgery with clinical features of blood stream sepsis do not have a positive blood culture and are managed as presumed ‘culture negative sepsis (CNS)’. There is little information on outcomes of CNS early after pediatric cardiac surgery. We sought to describe the incidence, outcomes and antibiotic utilization pattern of culture negative sepsis in children undergoing cardiac surgery.Methods : 437 consecutive children who underwent cardiac surgery were studied. CNS was empirically defined as those in whom antibiotics were upgraded based on clinical and/or laboratory suspicion of blood stream sepsis with eventual negative blood culture. Outcomes were compared between three groups: normal controls, CNS and Culture Positive Sepsis (CPS).Results: Incidence of CNS was 16% (71/437). The mortality was highest in CPS group (10.7%, 3/29); intermediate for CNS (2.9%, 2/71) and least for the normal group (1.2%, 4/337). Similarly, duration of ventilation and intensive care unit (ICU) length of stay (in hours) was highest for CPS (116 [45-271]; 288 [156-444]), intermediate for CNS (63 [23-112]; 192 [120-288]) and least for the normal group (18 [6-28]; 72 [48-120]). Third-tier antibiotics were initiated for 27 (40%) with CNS and 23 (92%) with CPS. Although the mean antibiotic duration for CNS (6.3±3.0 days) was less than CPS (9.09±5.12); p=0.022, 27.3% of CNS received antibiotics for more than one week. Conclusion: The high incidence of CNS points towards the need for accurate biomarkers of bacterial sepsis after cardiac surgery. The relatively better outcomes of CNS merits consideration to rapidly de-escalate antibiotics for presumed sepsis after cardiac surgery.
Rates of oropharyngeal cancer continue to rise steeply amongst Australian men: continued emphasis on HPV vaccination, tobacco and alcohol control
Authors: Fan Ka Ming , Sprague Samuel , Zhang Ping , Ariyawardana Anura , Johnson Newell W. 
Published: March 2022

Abstract
Objective
To analyse trends in incidence of oropharyngeal cancers (OPC) across Australia from 1982 to 2017 with implications for prevention.
Methods
Data were obtained from the Australian Cancer Database (ACD) compiled at the Australian Institute of Health and Welfare (AIHW). Incidence rates of OPC were expressed per 100,000 population, age-standardized in accordance with WHO standard population. Joinpoint analyses are presented.
Results
A striking increase of age-standardised incidence rate (ASIR) of OPC by over 1.5 times from 1.86/100,000 in 1982 to 3.23/100,000 in 2017 was observed in men: the most significant rise was between 2007 and 2017 with an annual percentage change (APC) of + 5.24% (p < 0.001). Slow but gradual growth of ASIR from 0.38/100,000 in 1982 to 0.71/100,000 in 2017 was observed among women with a statistically significant APC of + 1.02% (p < 0.001). Statistically significant bimodal increasing trends of APC were also observed in total ASIR of OPC. Highest number of incident cases were found in patients aged 55 to 69 years old, accounting for 50.8% on average of all ages during each 7-year time period. Most common subsites affected were base of tongue (BOT) and “oropharynx” from 1982 to 2017.
Conclusion
OPC is rising rapidly across Australia, particularly in men. Whilst the national proportion of cases driven by HPV is not known, it is evident that vaccination is yet to have an impact. A substantial proportion of cases are, presumably, still driven by traditional alcohol and tobacco habits, mandating continued efforts at control of these risk factors.
The heterogeneous early illness courses of Korean patients with bipolar disorders – replication of the stage model
Authors: Lee Yejin , Lee Dongbin , Jung Hyewon , Cho Yunji , Baek Ji Hyun , Hong Kyung Sue 
Published: March 2022

Abstract
Background
Clinical staging of bipolar disorder requires application of real-world data, as the next step in hypothesis. This study used the staging model to analyze the long-term course of BD in Korean patients based on clinical features and treatment responses to map the progression of bipolar illness from the early phase after the onset of illness.
Methods
A total of 136 patients diagnosed with BD-I (n = 62) or BD-II (n = 74) were recruited. Their progressive stages were retrospectively evaluated. The multi-state model was used to calculate the probability of progression to each stage. We calculated the hazard ratio of covariates expected to influence different courses of BD. Using the Alda score, we compared the long-term response to mood stabilizers depending on the current stage.
Results
Several sub-populations showed a varied course during the first five years after the onset of illness, with 41.5% remaining in stage 2 and 53% progressing to higher stages with shortening of time for transition. The profile of patients with BD-II differed profile from BD-I, suggesting biologically distinct groups. The group with Obsessive-compulsive Disorder (OCD) had higher risk in relation to both recurrent course (stage 3a or 3b) and malignant course (stage 3c or 4). Early age of onset, shorter lifetime duration, older age at the start of medication, and poor response to lithium affect long-term therapeutic outcomes.
Conclusions
The staging model encompasses early to recurrent and chronic courses of BD. The stage progression pattern differed in BD-Ⅰ and BD-Ⅱ patients from the early phase. Previous findings related to long-term course of BD enabled expansion of model parameters.
Automated radiolabelling procedures for the preparation of OncoFAP-based radiopharmaceuticals for cancer imaging and therapy
Authors: Bartoli Francesco , Elsinga Philip , Nazario Luiza Reali , Zana Aureliano , Galbiati Andrea , Millul Jacopo , Migliorini Francesca , Cazzamalli Samuele , Neri Dario , Slart Riemer H.J.A. , Erba Paola Anna 
Published: March 2022

Abstract
FAP-targeted radiopharmaceuticals represent a breakthrough in cancer imaging and a viable option for therapeutic applications. OncoFAP, an ultra-high affinity ligand of FAP, has been recently discovered and clinically validated for PET imaging procedures in patients with solid malignancies. While more and more clinical validation is becoming available, the need for scalable and robust procedures for the preparation of this new class of radiopharmaceuticals continues to increase. In this article, we present the development of automated radiolabelling procedures with FASTlab 2 for the preparation of OncoFAP-based radiopharmaceuticals for cancer imaging and therapy. A new series of [68Ga]Ga-OncoFAP, [177Lu]Lu-OncoFAP and [18F]AlF-OncoFAP was produced with high-yields. Chemical and biochemical characterization after radiolabelling confirmed excellent stability, retention of high affinity for FAP and absence of radiolysis by-products. The in vivo biodistribution of [18F]AlF-OncoFAP-NOTA, a candidate for PET imaging procedures in patients, was assessed in mice bearing FAP-positive solid tumours. The product showed rapid accumulation in solid tumours, with an average of 6.6% ID/g one hour after systemic administration and excellent tumour-to-healthy organs ratio. We have established methodologies to efficiently produce an OncoFAP-based radiotheranostic pair in which [68Ga]Ga-OncoFAP-DOTAGA is used as a companion diagnostic of the targeted therapeutic agent [177Lu]Lu-OncoFAP-DOTAGA Objectives: We have developed simple, quick, safe and robust synthetic procedures for the preparation of theranostic OncoFAP-compounds based on Gallium-68, Lutetium-177 and Alluminium-Fluoride-18 using the commercially available FASTlab 2 synthesis module.
Men of refugee and migrant backgrounds resettled in Australia: A scoping review of sexual and reproductive health research
Authors: Mengesha Zelalem , Hawkey Alex , Baroundi Mazen , Ussher Jane , Perz Janette 
Published: March 2022

Abstract
Background
Australia’s National Men’s Health Strategy 2020–2030 identifies men from refugee and migrant backgrounds as priority groups for sexual and reproductive health (SRH) interventions due to their high risk of poor outcomes. Given the traditional focus of SRH research on women, the lack of SRH research focusing on refugee and migrant men is a significant gap to guide implementation of the strategy and future research into this topic. To provide a foundation for rectifying this omission, this review aimed to synthesise the available evidence on refugee and migrant men’s SRH needs, understanding, and experiences of accessing services after resettlement in Australia.
Methods
We conducted a systematic search of peer reviewed literature in PubMed, Scopus, PsyInfo and Embase. A WHO framework for operationalising sexual health and its relationship with reproductive health was used to map the identified studies. The socio-ecological framework was applied to thematically synthesise data extracted from individual studies and identify factors that influence the SRH of refugee and migrant men.
Results
We identified and included 38 papers (24 sexual health, 5 reproductive health, 3 intimate relationships, 2 cancer and 4 ‘other’). The majority of sexual health studies (16) were about sexually transmitted infections (STIs), mainly HIV (12), Hepatitis B (3), and safe sexual practices (1). Reproductive health studies focused on contraceptive counselling and provision (3), antenatal, intrapartum and postnatal care (1) and safe abortion care (1). We also identified several factors influencing refugee and migrant men’s SRH including a lack of access to SRH information at the individual level, language and communication barriers at the interpersonal level and stigma at the community level.
Conclusions
This review indicates that the SRH literature on refugee and migrant men lacks perspectives beyond STIs. The literature does not frame SRH as a human right rather follows a risk-based biomedical approach of health care keeping important aspects of SRH, such as gender-based violence and sexual pleasure, out of health services research. These identified gaps should be addressed to develop effective, equitable and gender sensitive SRH programs for refugee and migrant men in Australia.