bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of genomic alterations in cancer associated human pancreatic stellate cells
Authors: Böker Viktoria , Häußler Johanna , Baumann Jenny , Sunami Yoshiaki , Trojanowicz Bogusz , Harwardt Bernadette , Hammje Kathrin , von Auw Nadine , Erkan Mert , Krohn Knut , Kleeff Jörg 
Published: August 2022

AbstractPancreatic stellate cells (PSCs) constitute important cells of the pancreatic microenvironment and their close interaction with cancer cells is important in pancreatic cancer. It is currently not known whether PSCs accumulate genetic alterations that contribute to tumor biology. Our aim was to analyze genetic alterations in cancer associated PSCs. PSC DNA was matched to DNA isolated from pancreatic cancer patients’ blood (n = 5) and analyzed by Next-Generation Sequencing (NGS). Bioinformatic analysis was performed using the GATK software and pathogenicity prediction scores. Sanger sequencing was carried out to verify specific genetic alterations in a larger panel of PSCs (n = 50). NGS and GATK analysis identified on average 26 single nucleotide variants in PSC DNA as compared to the matched blood DNA that could be visualized with the Integrative Genomics Viewer. The absence of PDAC driver mutations (KRAS, p53, p16/INK4a, SMAD4) confirmed that PSC isolations were not contaminated with cancer cells. After filtering the variants, using different pathogenicity scores, ten genes were identified (SERPINB2, CNTNAP4, DENND4B, DPP4, FGFBP2, MIGA2, POLE, SNRNP40, TOP2B, and ZDHHC18) in single samples and confirmed by Sanger sequencing. As a proof of concept, functional analysis using control and SERPINB2 knock-out fibroblasts revealed functional effects on growth, migration, and collagen contraction. In conclusion, PSC DNA exhibit a substantial amount of single nucleotide variants that might have functional effects potentially contributing to tumor aggressiveness.
RNA editing facilitates the enhanced production of neoantigens during the simultaneous administration of oxaliplatin and radiotherapy in colorectal cancer
Authors: Komatsu Yasuhiro , Shigeyasu Kunitoshi , Yano Shuya , Takeda Sho , Takahashi Kazutaka , Hata Nanako , Umeda Hibiki , Yoshida Kazuhiro , Mori Yoshiko , Yasui Kazuya , Yoshida Ryuichi , Kondo Yoshitaka , Kishimoto Hiroyuki , Teraishi Fuminori , Umeda Yuzo , Kagawa Shunsuke , Michiue Hiroyuki , Tazawa Hiroshi , Goel Ajay , Fujiwara Toshiyoshi 
Published: August 2022

AbstractMost cases of colorectal cancers (CRCs) are microsatellite stable (MSS), which frequently demonstrate lower response rates to immune checkpoint inhibitors (ICIs). RNA editing produces neoantigens by altering amino acid sequences. In this study, RNA editing was induced artificially by chemoradiation therapy (CRT) to generate neoantigens in MSS CRCs. Altogether, 543 CRC specimens were systematically analyzed, and the expression pattern of ADAR1 was investigated. In vitro and in vivo experiments were also performed. The RNA editing enzyme ADAR1 was upregulated in microsatellite instability–high CRCs, leading to their high affinity for ICIs. Although ADAR1 expression was low in MSS CRC, CRT including oxaliplatin (OX) treatment upregulated RNA editing levels by inducing ADAR1. Immunohistochemistry analyses showed the upregulation of ADAR1 in patients with CRC treated with CAPOX (capecitabine + OX) radiation therapy relative to ADAR1 expression in patients with CRC treated only by surgery (p < 0.001). Compared with other regimens, CRT with OX effectively induced RNA editing in MSS CRC cell lines (HT29 and Caco2, p < 0.001) via the induction of type 1 interferon-triggered ADAR1 expression. CRT with OX promoted the RNA editing of cyclin I, a neoantigen candidate. Neoantigens can be artificially induced by RNA editing via an OX–CRT regimen. CRT can promote proteomic diversity via RNA editing.
Phenylalanine suppresses cell death caused by loss of fumarylacetoacetate hydrolase in Arabidopsis
Authors: Jiang Yihe , Zhu Qi , Yang Hua , Zhi Tiantian , Ren Chunmei 
Published: August 2022

AbstractFumarylacetoacetate hydrolase (FAH) catalyzes the final step of Tyrosine (Tyr) degradation pathway essential to animals and the deficiency of FAH causes an inborn lethal disease. In plants, a role of this pathway was unknown until we found that mutation of Short-day Sensitive Cell Death1 (SSCD1), encoding Arabidopsis FAH, results in cell death under short day. Phenylalanine (Phe) could be converted to Tyr and then degraded in both animals and plants. Phe ingestion in animals worsens the disease caused by FAH defect. However, in this study we found that Phe represses cell death caused by FAH defect in plants. Phe treatment promoted chlorophyll biosynthesis and suppressed the up-regulation of reactive oxygen species marker genes in the sscd1 mutant. Furthermore, the repression of sscd1 cell death by Phe could be reduced by α-aminooxi-β-phenylpropionic acid but increased by methyl jasmonate, which inhibits or activates Phe ammonia-lyase catalyzing the first step of phenylpropanoid pathway, respectively. In addition, we found that jasmonate signaling up-regulates Phe ammonia-lyase 1 and mediates the methyl jasmonate enhanced repression of sscd1 cell death by Phe. These results uncovered the relation between chlorophyll biosynthesis, phenylpropanoid pathway and jasmonate signaling in regulating the cell death resulting from loss of FAH in plants.
Geographic isolation drives speciation in Nearctic aphids
Authors: Hardy Nate B. , Kaczvinsky Chloe , Bird Gwendolyn , Richter Robin , Dettman Jeremy R. , Maw Eric , Brunet Bryan M. T. , Foottit Robert G. 
Published: August 2022

AbstractAcross herbivorous insect clades, species richness and host-use diversity tend to positively covary. This could be because host-use divergence drives speciation, or because it raises the ecological limits on species richness. To evaluate these hypotheses, we performed phylogenetic path model analyses of the species diversity of Nearctic aphids. Here, we show that variation in the species richness of aphid clades is caused mainly by host-use divergence, whereas variation in speciation rates is caused more by divergence in non-host-related niche variables. Aphid speciation is affected by both the evolution of host and non-host-related niche components, but the former is largely caused by the latter. Thus, our analyses suggest that host-use divergence can both raise the ecological limits on species richness and drive speciation, although in the latter case, host-use divergence tends to be a step along the causal path leading from non-host-related niche evolution to speciation.
Interference and secrecy analysis based on randomly spacial model in clustered WSNs
Authors: Tong Fei , Zhu Tian , Peng Yuyang 
Published: August 2022

AbstractClustering and layering are widely employed in large‐scale wireless sensor networks (WSNs) to alleviate data implosion, reduce transmission delay and improve energy efficiency. It is vital to conduct performance analysis in clustered WSNs for better defining and designing networks. Here, a general analytical framework based on a randomly spacial model is proposed to conduct inter‐cluster interference analysis and physical‐layer security analysis for clustered WSNs. It is assumed that two adjacent cluster heads exchange confidential massage with a passive eavesdropper and the non‐head nodes in clusters could be selected as cooperative jamming (CJ) nodes to disturb the eavesdropper. All mentioned nodes are randomly located in their specific areas. Under the above settings, three scenarios, namely interference, eavesdropping and CJ are considered, and a stochastic geometry tool based on kinematic measure is employed for analysis. Comparing to existing stochastic geometry methods, the proposed analytical framework can handle arbitrarily shaped, disjoint and tiered networks. The results obtained from extensive simulations have verified the rationality of the framework and demonstrated the impact of different parameters on the performance metrics of interest. The comparison with PPP also shows the advantages of this proposed model.
Grid forming inverter and its applications to support system strength–A case study
Authors: Zhou Yi , Zhang Rui , Kathriarachchi Dinesh , Dennis Jonathan , Goyal Sachin 
Published: August 2022

AbstractWith the increasing penetration level of renewable generation, a shortage of system strength becomes a concern for the stable operation of the power system. Most commonly, Inverter Based Resources (IBR) plants are operated with grid following inverters (GFLI). However, a grid forming inverter (GFMI), which work as a voltage source and does not require direct reference and system strength from the grid, is now receiving increased attention. Here, Hardware‐in‐the‐loop (HIL) testing of a GFMI and its capability to actively damp sub‐synchronous oscillations and to provide synthetic inertia has been discussed. The improvement to system strength and transient stability have been presented using modelling of the transmission network in the Queensland (Australia). The study shows that with proper tuning and coordination with other generation in the area, the GFMI BESS will not only provide system strength to the grid, but also solve other stability issues.
HD 23472: a multi-planetary system with three super-Earths and two potential super-Mercuries,
Authors: Barros S. C. C. , Demangeon O. D. S. , Alibert Y. , Leleu A. , Adibekyan V. , Lovis C. , Bossini D. , Sousa S. G. , Hara N. , Bouchy F. , Lavie B. , Rodrigues J. , da Silva J. Gomes , Lillo-Box J. , Pepe F. A. , Tabernero H. M. , Osorio M. R. Zapatero , Sozzetti A. , Mascareño A. Suárez , Micela G. , Prieto C. Allende , Cristiani S. , Damasso M. , Di Marcantonio P. , Ehrenreich D. , Faria J. , Figueira P. , Hernández J. I. González , Jenkins J. , Lo Curto G. , Martins C. J. A. P. , Micela G. , Nunes N. J. , Pallé E. , Santos N. C. , Rebolo R. , Seager S. , Twicken J. D. , Udry S. , Vanderspek R. , Winn J. N. 
Published: August 2022

Context.Comparing the properties of planets orbiting the same host star, and thus formed from the same accretion disc, helps in constraining theories of exoplanet formation and evolution. As a result, the scientific interest in multi-planetary systems is growing with the increasing number of detections of planetary companions.Aims.We report the characterisation of a multi-planetary system composed of five exoplanets orbiting the K-dwarf HD 23472 (TOI-174).Methods.In addition to the two super-Earths that were previously confirmed, we confirm and characterise three Earth-size planets in the system using ESPRESSO radial velocity observations. The planets of this compact system have periods ofPd~3.98,Pe~7.90,Pf~12.16,Pb~ 17.67, andPc~ 29.80 days and radii ofRd~ 0.75 ,Re~ 0.82,,Rf~ 1.13 ,Rb~ 2.01, and,Rc~1.85R⊕.Because of its small size, its proximity to planet d’s transit, and close resonance with planet d, planet e was only recently found.Results.The planetary masses were estimated to beMd= 0.54 ± 0.22,Me= 0.76 ± 0.30,Mf= 0.64−0.39+0.46,Mb= 8.42−0.84+0.83, andMc= 3.37−0.87+0.92M⊕. These planets are among the lightest planets, with masses measured using the radial velocity method, demonstrating the very high precision of the ESPRESSO spectrograph. We estimated the composition of the system’s five planets and found that their gas and water mass fractions increase with stellar distance, suggesting that the system was shaped by irradiation. The high density of the two inner planets (ρd= 7.5−3.1+3.9andρe= 7.5−3.0+3.9g cm−3) indicates that they are likely to be super-Mercuries. This is supported by the modelling of the internal structures of the planets, which also suggests that the three outermost planets have significant water or gas content.Conclusions.If the existence of two super-Mercuries in the system is confirmed, this system will be the only one known to feature two super-Mercuries, making it an excellent testing bed for theories of super-Mercuries formation. Furthermore, the system is close to a Laplace resonance, and further monitoring could shed light on how it was formed. Its uniqueness and location in the continuous viewing zone of theJames Webbspace telescope will make it a cornerstone of future in-depth characterisations.
A slow bar in the lenticular barred galaxy NGC 4277
Authors: Buttitta C. , Corsini E. M. , Cuomo V. , Aguerri J. A. L. , Coccato L. , Costantin L. , Dalla Bontà E. , Debattista V. P. , Iodice E. , Méndez-Abreu J. , Morelli L. , Pizzella A. 
Published: August 2022

Aims.We characterised the properties of the bar hosted in lenticular galaxy NGC 4277, which is located behind the Virgo cluster.Methods.We measured the bar length and strength from the surface photometry obtained from the broad-band imaging of the Sloan Digital Sky Survey and we derived the bar pattern speed from the stellar kinematics obtained from the integral-field spectroscopy performed with the Multi Unit Spectroscopic Explorer at the Very Large Telescope. We also estimated the co-rotation radius from the circular velocity, which we constrained by correcting the stellar streaming motions for asymmetric drift, and we finally derived the bar rotation rate.Results.We found that NGC 4277 hosts a short (Rbar = 3.2−0.6+0.9kpc), weak (Sbar = 0.21 ± 0.02), and slow (ℛ = 1.8−0.3+0.5) bar and its pattern speed (Ωbar = 24.7 ± 3.4 km s−1kpc−1) is amongst the best-constrained ones ever obtained with the Tremaine–Weinberg (TW) method with relative statistical errors of ∼0.2.Conclusions.NGC 4277 is the first clear-cut case of a galaxy hosting a slow stellar bar (ℛ > 1.4 at more than a 1σconfidence level) measured with the model-independent TW method. A possible interaction with the neighbour galaxy NGC 4273 could have triggered the formation of such a slow bar and/or the bar could be slowed down due to the dynamical friction with a significant amount of dark matter within the bar region.
Krebstherapien und Herzerkrankungen – ein komplexes Wechselspiel
Authors: Birner Christoph 
Published: August 2022

ZusammenfassungMit der zunehmenden Entwicklung hoch effektiver onkologischer Therapien sind nun
zahlreiche Krebserkrankungen mit einer deutlich besseren Langzeitprognose
assoziiert. Aus diesem Grund ist es zwingend erforderlich, potenzielle
kardiotoxische Nebenwirkungen dieser Therapien möglichst frühzeitig zu
identifizieren, um das Langzeitüberleben von Krebspatienten nicht durch
schwerwiegende, schlimmstenfalls sogar letale kardiovaskuläre Ereignisse zu
belasten. Eine wichtige Rolle spielen dabei insbesondere medikamentös induzierte
Kardiomyopathien, aber auch Arrhythmien, eine verschlechterte arterielle
Hypertonie und arterielle bzw. venöse Thrombosen müssen berücksichtigt werden.
Durch adaptierte Ausgangs- und Verlaufsuntersuchungen lassen sich Patienten
identifizieren, die ein erhöhtes Risiko für derartige kardiotoxische Ereignisse
haben. Auch wenn die Datenlage inkonklusiv ist, scheint es doch kardioprotektive
Medikamente zu geben, die in dieser Situation angewandt werden können.
Differential Diagnosis of Keratoconus Based on New Technologies
Authors: Sideroudi Haris , Flockerzi Elias , Seitz Berthold 
Published: August 2022

AbstractKeratoconus (KC) must be distinguished from other corneal ectatic diseases and thinning disorders for stage-appropriate and suitable management of each condition. The most relevant corneal
pathologies that may imitate the tomographic KC pattern are pellucid marginal degeneration (PMD), keratoglobus, posterior keratoconus, and Fuchs-Terrien marginal degeneration (FTMD). In
moderate cases of KC, differentiation is typically possible using slit lamp examination and corneal tomography with evaluation of the location of the corneal thinning region. In early cases,
however, differential diagnosis may be more challenging since the cornea may look relatively normal. In severe cases, the extended area of corneal thinning also complicates differentiation.
Biomicroscopic findings cannot always give all the information needed to distinguish KC from related ectatic corneal conditions. The aim of this work is to discuss contemporary techniques
and findings to assist physicians to identify the correct diagnosis. Corneal topography has been used in recent decades as the main tool for imaging in ectatic corneal diseases. Moreover,
Scheimpflug cameras (corneal tomographers), which analyze both anterior and posterior corneal surfaces, curvatures, pachymetry, elevation data, higher order aberrations, Fourier analysis of
keratometric data, and corneal density have become the most promising tools for diagnosis and follow-up of ectatic diseases. A noninvasive air pulse tonometer in conjunction with an
ultrahigh-speed Scheimpflug camera complements tomographic findings by analyzing biomechanical corneal properties. Α confocal microscopy system, which is a novel clinical technique for the
study of corneal cellular structure, could contribute effectively in the same direction. Moreover, anterior segment optical coherence tomography (AS-OCT) creates cross-sections, which can be
generated into a three-dimensional structure to produce corneal epithelial thickness (ET) measurements. ET mapping is increasingly recognized as a sensitive tool for the diagnosis of ocular
surface disorders. Combining information of all these systems could lead to a more effective identification and differential diagnosis of ectatic corneal disorders.
Transition-Metal-Free N-(o-Halo)arylation of Sulfoximines/Sulfonimidamides
Authors: Nandi Ganesh Chandra , Padma Priya V. R. , Irfana Jesin C. P. 
Published: August 2022

AbstractA transition-metal-free novel route to access N-(o-halo)arylsulfoximines/sulfonimidamides is achieved by the reaction of sulfoximine/sulfonimidamide, aryne precursor, and CCl4/CBr4 in the presence of KF/18-crown-6. The in situ generated benzyne intermediate (from silyl aryl triflate) reacts with the nucleophile (sulfoximine/sulfonimidamide) and halide source (CCl4/CBr4) to yield the product in moderate to good yield. The protocol exhibits broad substrate scope. The regioisomers formed from the unsymmetric aryne precursor were separated effectively via column chromatography.
Outcome of Digital Cholangioscopy and Laser Lithotripsy for Impacted Biliary Stones
Authors: Singla Vikas , Arora Anil , Bopanna Sawan , Khare Shivam , Kumar Ashish , Bansal Naresh , Sharma Praveen 
Published: August 2022

Abstract
Background and Aims Impacted stones in the bile duct are difficult to extract, and are predictor of failure of conventional endoscopic retrograde cholangiopancreatography techniques including mechanical lithotripsy and large balloon dilatation. Intracorporeal lithotripsy may be an effective technique for these stones. The aim of this study is to report the efficacy and safety of intracorporeal laser lithotripsy for impacted stones in the bile duct.
Method This study is retrospective analysis of prospectively collected data. Patients with impacted stones in the bile duct underwent cholangioscopy with spyglass DS system and laser lithotripsy. Outcome measures were proportion of patients with complete clearance of bile duct after the first session, number of sessions required for complete clearance, and the complications.
Results Forty-three patients (27 female) with mean age of 56.12 ± 15.16 years underwent digital cholangioscopy and laser lithotripsy. Mean bilirubin value was 1.8 ± 1.6 mg/dL, 20 (46.51%) patients had single stone, 35(81.39%) patients had only bile duct stones, and 8(18.61%) patients had additional stones in cystic duct or intrahepatic biliary radical. Mean size of largest stone was 16.2 ± 4.4mm. Average duration of the procedure was 69.11 ± 28.12minutes, and complete clearance was achieved in 41/43 (95.34%) patients after the first session. Mean number of sessions required for complete clearance was 1.02 ± .26. Postprocedure cholangitis occurred in one patient.
Conclusion Intracorporeal laser lithotripsy is an effective and safe modality for the clearance of impacted bile duct stones.
On the thermodynamic properties of fictitious identical particles and the application to fermion sign problem
Authors: Xiong Yunuo , Xiong Hongwei 
Published: August 2022

By generalizing the recently developed path integral molecular dynamics for identical bosons and fermions, we consider the finite-temperature thermodynamic properties of fictitious identical particles with a real parameter ξ interpolating continuously between bosons (ξ = 1) and fermions (ξ = −1). Through general analysis and numerical experiments, we find that the average energy may have good analytical properties as a function of this real parameter ξ, which provides the chance to calculate the thermodynamical properties of identical fermions by extrapolation with a simple polynomial function after accurately calculating the thermodynamic properties of the fictitious particles for ξ ≥ 0. Using several examples, it is shown that our method can efficiently give accurate energy values for finite-temperature fermionic systems. Our work provides a chance to circumvent the fermion sign problem for some quantum systems.
Proximization and dialogue in Internet news texts and comments
Authors: Kowalski Grzegorz 
Published: August 2022

AbstractThe paper presents the results of an empirical study on proximization approached as a dialogue between journalists and readers of Internet media texts and online comments. In this way the paper shows a practical application of the research programme, whose tenets I presented elsewhere (Kowalski 2018), in relation to Polish and Romanian reciprocal media coverage on the winter 2016/2017 protests in the two countries, and follow-up online comments. The analysis shows that participants used proximization for different communicative purposes (descriptive or evaluative), for which they employed different reportoires of proximization strategies (categorization, domestication, construing hybrid IDC/ODC worlds, historical analogies, and references to cultural stereotypes). As a result, there was no consensus on a shared Discourse Space, and alternative Discourse Spaces were negotiated in the online dialogue.
Shifting addressivity
Authors: Caronia Letizia , Ranzani Federica , Colla Vittoria 
Published: August 2022

Abstract
In dealing with recent migration-related phenomena, inclusion has become an increasingly common
normative ethical imperative in socio-political discourse. Considering inclusion as a situated interactive
accomplishment, this article reports findings from a study on medical visits, each one involving a physician,
an unaccompanied foreign minor (UFM) and a professional educator. Adopting a Conversation Analysis-informed
approach to a corpus of video-recorded visits, we analyze (a) the physician’s shifts in addressivity, which
either foster or hinder UFM’s inclusion during the history-taking phase, and b) when and
how these shifts occur. We contend that, by shifting addressivity, the physician
navigates the locally incompatible goals of gaining reliable information on UFM patients and fostering their
active participation. We contend that the micro-practice of shifting addressivity is consistent with the
management of cultural-linguistic diversity proposed by the intercultural dialogue perspective.
Practices of inclusion/exclusion in and through classroom dialogue
Authors: Nasi Nicola 
Published: August 2022

AbstractThe paper explores non-native children’s peer socialization to norms of literacy and appropriate language use in the classroom. Drawing on ethnographic research in a primary school in northern Italy, this study adopts a CA-informed approach to analyze an Italian L2 class attended by children aged 8 to 10. The study focuses on children’s enacting of correction sequences following peers’ problematic conduct. As the analysis illustrates, children creatively re-produce teachers’ ways of speaking to enforce normative uses of language. Through these practices, non-native children socialize their classmates into expected ways of speaking, reading, and writing, and negotiate the social hierarchy of the peer group. Risks and opportunities of such practices are considered in relation to children’s social inclusion and exclusion.