bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical grade production of IL‐15 stimulated NK cells for early infusion in adult AML patients undergoing haploidentical stem cell transplantation with post‐transplant cyclophosphamide
Authors: Rubio‐Azpeitia Eva , Pérez‐Corral Ana Maria , Dorado‐Herrero Nieves , Monsalvo Silvia , Pérez‐Balsera Gonzalo , Fernández‐Santos Maria Eugenia , Kwon Mi , Oarbeascoa Gillen , Bastos‐Oreiro Mariana , Falero Carmen , Pascual Izquierdo Cristina , Muñoz‐Martínez Cristina , Pérez‐Martínez Antonio , Diez‐Martin José Luis , Anguita Javier 
Published: January 2022

AbstractBackgroundAllogeneic stem cell transplantation is the treatment of choice for acute myeloid leukemia (AML) patients. Unmanipulated haploidentical transplantation (Haplo‐HSCT) is commonly used for those AML patients who need a timely transplant and do not have a suitable matched donor, but relapse rates are still high, and improvements are needed. Adoptive immunotherapy using natural killer cells (NK cells) could be a promising tool to improved Haplo‐HSCT but, to date, no optimal infusion and manufacturing protocols have been developed.Study Design and MethodsIn this study, we describe a quick and reproducible protocol for clinical‐grade production of haploidentical donor NK cells using double immunomagnetic depletion and enrichment protocol and overnight IL‐15 stimulation.ResultsThus, we have obtained 8 viable and functional NK cell products that have been safely infused to five AML patients undergoing unmanipulated Haplo‐HSCT.DiscussionOur results demonstrate the safety and feasibility of manufactured NK IL15 cells obtained from an adult allogeneic donor in the setting of haploidentical transplantation for AML patients.
Impact of the pathogen inactivation process on the migration of di(2‐ethylhexyl) phthalate from plasma bags
Authors: Thelliez Aurélie , Décaudin Bertrand , Lecoeur Marie 
Published: January 2022

AbstractBackground and ObjectivesDi(2‐ethylhexyl) phthalate (DEHP) is a toxic plasticizer that is commonly used in the manufacture of polyvinyl chloride (PVC) blood bags. It is well known that DEHP can migrate from a medical device into the blood plasma. For safety reasons, pathogens in plasma must be inactivated; however, this process may increase DEHP migration. Here, we assessed the impact of illumination‐based pathogen inactivation on the migration of DEHP from PVC bags into plasma.Materials and MethodsPairs of native PVC‐DEHP plasma bags were pooled. Each pool was then split into a pathogen‐inactivated bag and a control bag. After illumination, the plasma concentrations of DEHP and its main metabolite (mono(2‐ethylhexyl) phthalate, MEHP) in each bag were assayed and compared using liquid chromatography–tandem mass spectrometry. Concentrations were evaluated in repeated‐measures, two‐way analyses of variance.ResultsThe MEHP concentration was significantly associated with storage but not with illumination (p = 0.0001). The DEHP concentration stayed constant throughout the storage period. The DEHP equivalent concentration (corresponding to the overall plasticizer migration rate into plasma) was not significantly associated with illumination (p = 0.3) or storage (p = 0.09; mean ± standard deviation of the mean DEHP concentration for all conditions: 147.9 ± 11.3 μg/ml).ConclusionIllumination‐based inactivation of pathogens in plasma did not increase the DEHP equivalent concentration, relative to control (non‐inactivated) plasma.
Rapid genotyping of 508G>A (rs3745635) and 1067T>A (rs3894326) of FUT3 by a duplex Eprobe‐mediated melting curve analysis
Authors: Soejima Mikiko , Koda Yoshiro 
Published: January 2022

AbstractBackground and ObjectivesLewis histo‐blood group phenotypes are regulated by the action of FUT3‐encoded α(1,3/1,4)fucosyltransferase and FUT2‐encoded α(1,2)fucosyltransferase. Since Lewis phenotypes are suggested to be associated with various clinical conditions, a method for large‐scale FUT3 genotyping is desirable. In worldwide populations, 508G>A and 1067T>A of FUT3 are two of three common causal single nucleotide polymorphisms for Lewis‐negative alleles.Materials and MethodsWe developed a duplex Eprobe‐mediated melting curve analysis for genotyping 508G>A and 1067T>A simultaneously and applied this method to 106 Ghanaian and 140 Japanese subjects.ResultsThe results of both 508G>A and 1067T>A genotyping by duplex Eprobe‐mediated melting curve analysis were completely in agreement with the results of a DNA sequence analysis in 106 Ghanaians and polymerase chain reaction‐restriction fragment length polymorphism analysis in 140 Japanese subjects.ConclusionThe present duplex Eprobe‐mediated melting curve analysis is valid and credible for large‐scale estimation of Lewis‐negative alleles.
Influence of crotch suture augmentation on leakage pressure and leakage location during functional end‐to‐end stapled anastomoses in dogs
Authors: Duffy Daniel J. , Chang Yi‐Jen , Moore George E. 
Published: January 2022

AbstractObjectiveTo determine the influence of anastomotic crotch suture augmentation on leakage pressures and leakage location following intestinal functional end‐to‐end stapled anastomosis (FEESA) in dogs.Study designEx vivo, randomized, experimental.Sample populationChilled jejunal segments from 3 adult dogs.MethodsJejunal specimens were tested within 24 hours of collection. A FEESA was performed and randomly assigned to 1 of 4 treatment groups (n = 12/group): (1) no crotch suture (NCS); (2) simple interrupted crotch suture (SICS); (3) two simple interrupted crotch sutures (TCS) placed laterally on opposing jejunal limbs; (4) simple continuous crotch suture (SCCS) augmentation. Crotch sutures were performed using 3‐0 USP polydioxanone. Initial (ILP) and maximal (MLP) leakage pressures (Mean ± SD mm Hg) and leakage location were recorded and compared between groups.ResultsInitial leakage pressure was greater after placement of TCS (37.8 ± 6.4,P < .039) and SCCS (47.6 ± 11.0,P < .002) than NCS (27.1 ± 2.5) and SICS (33.0 ± 6.0). Maximal leakage pressure was greater in specimens including SICS, TCS, and SCCS than those without crotch suture augmentation (P < .043). Leakage occurred at the anastomotic crotch in 8/12 NCS, 6/12 SICS, 11/12 TCS, and 12/12 SCCS constructs (P < .001).ConclusionAugmentation of FEESA with TCS and SCCS increased ILP and decreased the occurrence of leakage from the anastomotic crotch, while all methods of anastomotic crotch augmentation increased MLP.Clinical significanceAugmenting the FEESA with crotch suture(s) improved the resistance of the jejunal anastomosis to leakage in normal cadaveric segments. Placing 2 crotch sutures or use of a simple continuous pattern for anastomotic augmentation appeared to be superior to the placement of a single suture.
Perceptions and experiences of health care professionals and staff with animal-assisted interventions in health care settings: a qualitative systematic review protocol
Authors: Beck Amy J. , Barber Tanya , McKenzie Holly , Thorlakson Jessica , Dell Colleen , Keeping-Burke Lisa 
Published: January 2022

ABSTRACT

Objective:
The objective of this review is to examine the perceptions and experiences of health care professionals and staff in implementing or coordinating animal-assisted interventions in health care settings.

Introduction:
Animal-assisted interventions are applied in the areas of health, education, and human services to help improve individuals’ health and wellness. The positive effects of animal-assisted interventions on individuals have been shown in multiple health disciplines, from pediatrics to long-term care, and include outcomes such as decreased feelings of loneliness and increased feelings of support. The increase of animal-assisted interventions in human health has initiated growing research on health care professionals’ perceptions and experiences of these interventions. No current qualitative systematic reviews have focused solely on health care professionals’ and staff's perceptions of animal-assisted interventions. Conducting such a review will advance understanding of how these providers perceive and engage with animal-assisted interventions as well as their influence and role in coordinating these interventions.

Inclusion criteria:
This review will consider qualitative primary studies that address the perceptions and experiences of health care professionals and staff in implementing or coordinating animal-assisted interventions in health care settings.

Methods:
Nine bibliographic databases will be systematically searched for published and unpublished studies by employing a three-step search strategy. Studies published from database inception to present and in English will be considered. Two reviewers will independently appraise the studies and extract qualitative data using the standardized JBI critical appraisal and data extraction instruments. Findings from the review will be categorized according to similarity in meaning, and categories will be subjected to a meta-synthesis to produce a single comprehensive set of synthesized findings.

Systematic review registration number:
PROSPERO CRD42021258909
A Surface Defect Detection Method Via Fusing Multi-Level Features
Authors: Ren Meijian , Shen Rulin , Gong Yanling 
Published: January 2022

Abstract
Surface defect detection is very important to ensure product quality, but most of the surface defects of industrial products are characterized by low contrast, large variation in size and category similarity, which brings challenges to the automatic detection of defects. To solve these problems, this paper proposes a defect detection method based on convolutional neural network. In this method, a backbone network with semantic supervision is applied to extract the features of different levels. While a multi-level feature fusion module is proposed to fuse adjacent feature maps into high-resolution feature maps successively, it significantly improves the prediction accuracy of the network. Finally, an encoding module is used to obtain the global context information of the high-resolution feature map, which further improves the pixel classification accuracy. Experiments show that the mean intersection of union (mIoU) of the proposed method is superior to other methods on a standardized defect detection dataset of steel strip (NEU_SEG, mIoU of 85.27%) and a magnetic-tile defect dataset (mIoU of 77.82%).
Do health literacy levels of nursing students change throughout the study programme? A cross-sectional study
Authors: González-López José Rafael , Rodríguez-Gázquez María de los Angeles 
Published: January 2022

ObjectivesThe aim of this research was to analyse if the level of health literacy (HL) of nursing students changes throughout the study programme.DesignA cross-sectional study with anonymous self-reporting was conducted.Participants/setting329 public university nursing students in Seville, Spain; 243 of the first year and 86 of the fourth.InterventionsThe short Spanish version of the Health Literacy Survey Questionnaire-European Union was used to evaluate HL.Primary and secondary outcome measuresThe proportions of the limited level of HL were compared between academic years and the crude and corrected OR were calculated with the Mantel-Haenszel test to evaluate the effect of confusion of the sex variable on the HL level and academic year relationship. A logistic regression model with step-by-step analysis was run, including the independent variables sex, age, marital status, academic year and HL level (limited/sufficient) as the dependent variable.Results62.1% of the participants of the first year versus 47.7% of the fourth year had a limited literacy level for a crude OR of 1.5 (95% CI 1.10 to 2.21; p=0.014) and a corrected by sex OR of 1.8 (95% CI 1.10 to 2.96; p=0.026). Only the strata in women had a statistically significant relationship. The logistic regression model ratified that the HL was a function exclusive to the academic year.ConclusionThe HL level of nursing students increases from the first to the fourth academic year, even when controlling for sex. Although the HL level in the fourth academic year was greater than that of the first, both groups had inadequate HL levels. It is hence recommended to implement intervention strategies, which reinforce in the curriculum the knowledge and experiences related with health communication and education to ensure that future professionals improve their HL. Achieving adequate HL is crucial to be able to provide care to patients, their families and the community.
Development and validation of a prehospital-stage prediction tool for traumatic brain injury: a multicentre retrospective cohort study in Korea
Authors: Choi Yeongho , Park Jeong Ho , Hong Ki Jeong , Ro Young Sun , Song Kyoung Jun , Shin Sang Do 
Published: January 2022

ObjectivesPredicting diagnosis and prognosis of traumatic brain injury (TBI) at the prehospital stage is challenging; however, using comprehensive prehospital information and machine learning may improve the performance of the predictive model. We developed and tested predictive models for TBI that use machine learning algorithms using information that can be obtained in the prehospital stage.DesignThis was a multicentre retrospective study.Setting and participantsThis study was conducted at three tertiary academic emergency departments (EDs) located in an urban area of South Korea. The data from adult patients with severe trauma who were assessed by emergency medical service providers and transported to three participating hospitals between 2014 to 2018 were analysed.ResultsWe developed and tested five machine learning algorithms—logistic regression analyses, extreme gradient boosting, support vector machine, random forest and elastic net (EN)—to predict TBI, TBI with intracranial haemorrhage or injury (TBI-I), TBI with ED or admission result of admission or transferred (TBI with non-discharge (TBI-ND)) and TBI with ED or admission result of death (TBI-D). A total of 1169 patients were included in the final analysis, and the proportions of TBI, TBI-I, TBI-ND and TBI-D were 24.0%, 21.5%, 21.3% and 3.7%, respectively. The EN model yielded an area under receiver–operator curve of 0.799 for TBI, 0.844 for TBI-I, 0.811 for TBI-ND and 0.871 for TBI-D. The EN model also yielded the highest specificity and significant reclassification improvement. Variables related to loss of consciousness, Glasgow Coma Scale and light reflex were the three most important variables to predict all outcomes.ConclusionOur results inform the diagnosis and prognosis of TBI. Machine learning models resulted in significant performance improvement over that with logistic regression analyses, and the best performing model was EN.
Perceived working mechanisms of psychosomatic therapy in patients with persistent somatic symptoms in primary care: a qualitative study
Authors: Wortman Margreet S H , Olde Hartman Tim C , van der Wouden Johannes C , Dankers Sarah , Visser Bart , Assendelft Willem J J , van der Horst Henriëtte E 
Published: January 2022

ObjectivesTo explore the perceived working mechanisms of psychosomatic therapy according to patients with persistent somatic symptoms (PSS) and their psychosomatic therapists.DesignQualitative study using semistructured face-to-face interviews and focus groups. All interviews were audiorecorded, transcribed verbatim and analysed, by two researchers independently, based on the thematic analysis.SettingAlongside a randomised controlled trial to establish the (cost-)effectiveness of psychosomatic therapy in patients with PSS in primary care, we conducted a process evaluation with a qualitative study. Patients were recruited in general practice in three regions in the Netherlands.ParticipantsInterviews were conducted with twenty patients with PSS who received psychosomatic therapy and 25 psychosomatic therapists. In addition, two focus groups were conducted with six and seven psychosomatic therapists, respectively.InterventionPsychosomatic therapy, delivered by specialised exercise and physical therapists, is a multimodal and tailored treatment based on the biopsychosocial model.Outcome measuresExperiences, opinions and views from patients’ and therapists’ perspective on psychosomatic therapy were identified.ResultsA total of 37 interviews with patients, 25 interviews and two focus groups with therapists were analysed. Three main themes emerged from the data of the patients: (1) continuous alternation of psychosocial conversations and body-oriented exercises; (2) awareness of body–mind connection and (3) good relationship with therapist. Four main themes emerged from the data of the therapists (1) building rapport; (2) continuously searching for common ground; (3) making patients aware of the interaction between body and mind; and (4) continuous alternation between exploration and treatment.ConclusionAccording to patients as well as therapists, the continuous alternation of psychosocial conversations and body-oriented exercises to provide awareness of the interaction between body and mind are the perceived working mechanism of psychosomatic therapy. Therapeutic alliance and finding common ground between patient and therapist are prerequisites for the success of psychosomatic therapy.Trial registration numberNL7157 (NTR7356).
Chemotherapy-induced infiltration of neutrophils promotes pancreatic cancer metastasis via Gas6/AXL signalling axis
Authors: Bellomo Gaia , Rainer Carolyn , Quaranta Valeria , Astuti Yuliana , Raymant Meirion , Boyd Elzbieta , Stafferton Ruth , Campbell Fiona , Ghaneh Paula , Halloran Christopher M , Hammond Dean E , Morton Jennifer P , Palmer Daniel , Vimalachandran Dale , Jones Robert , Mielgo Ainhoa , Schmid Michael C 
Published: January 2022

ObjectivePancreatic ductal adenocarcinoma (PDAC) is a highly metastatic disease and cytotoxic chemotherapy is the standard of care treatment for patients with advanced disease. Here, we investigate how the microenvironment in PDAC liver metastases reacts to chemotherapy and its role in metastatic disease progression post-treatment, an area which is poorly understood.DesignThe impact of chemotherapy on metastatic disease progression and immune cell infiltrates was characterised using flow and mass cytometry combined with transcriptional and histopathological analysis in experimental PDAC liver metastases mouse models. Findings were validated in patient derived liver metastases and in an autochthonous PDAC mouse model. Human and murine primary cell cocultures and ex vivo patient-derived liver explants were deployed to gain mechanistical insights on whether and how chemotherapy affects the metastatic tumour microenvironment.ResultsWe show that in vivo, chemotherapy induces an initial infiltration of proinflammatory macrophages into the liver and activates cytotoxic T cells, leading only to a temporary restraining of metastatic disease progression. However, after stopping treatment, neutrophils are recruited to the metastatic liver via CXCL1 and 2 secretion by metastatic tumour cells. These neutrophils express growth arrest specific 6 (Gas6) which leads to AXL receptor activation on tumour cells enabling their regrowth. Disruption of neutrophil infiltration or inhibition of the Gas6/AXL signalling axis in combination with chemotherapy inhibits metastatic growth. Chemotherapy increases Gas6 expression in circulating neutrophils from patients with metastatic pancreatic cancer and recombinant Gas6 is sufficient to promote tumour cell proliferation ex vivo, in patient-derived metastatic liver explants.ConclusionCombining chemotherapy with Gas6/AXL or neutrophil targeted therapy could provide a therapeutic benefit for patients with metastatic pancreatic cancer.
Efficacy ofHelicobacter pylorieradication therapy for functional dyspepsia: updated systematic review and meta-analysis
Authors: Ford Alexander C , Tsipotis Evangelos , Yuan Yuhong , Leontiadis Grigorios I , Moayyedi Paul 
Published: January 2022

ObjectiveFunctional dyspepsia (FD) is a chronic disorder that is difficult to treat.Helicobacter pylorimay contribute to its pathophysiology. A Cochrane review from 2006 suggested that eradication therapy was beneficial, but there have been numerous randomised controlled trials (RCTs) published since. We evaluated impact of eradication therapy on both cure and improvement of FD, as well as whether any benefit was likely to arise from eradication ofH. pylori.DesignWe searched the medical literature through October 2021 to identify RCTs examining efficacy of eradication therapy inH. pylori-positive adults with FD. The control arm received antisecretory therapy or prokinetics, with or without placebo antibiotics, or placebo alone. Follow-up was for ≥3 months. We pooled dichotomous data to obtain a relative risk (RR) of symptoms not being cured or symptoms not improving with a 95% CI. We estimated the number needed to treat (NNT).ResultsTwenty-nine RCTs recruited 6781H.pylori-positive patients with FD. Eradication therapy was superior to control for symptom cure (RR of symptoms not being cured=0.91; 95% CI 0.88 to 0.94, NNT=14; 95% CI 11 to 21) and improvement (RR of symptoms not improving=0.84; 95% CI 0.78 to 0.91, NNT=9; 95% CI 7 to 17). There was no significant correlation between eradication rate and RR of FD improving or being cured (Pearson correlation coefficient=−0.23, p=0.907), but the effect was larger in patients with successful eradication ofH. pylorithan with unsuccessful eradication (RR=0.65; 95% CI 0.52 to 0.82, NNT=4.5, 95% CI 3 to 9). Adverse events (RR=2.19; 95% 1.10 to 4.37) and adverse events leading to withdrawal (RR=2.60; 95% CI 1.47 to 4.58) were more common with eradication therapy.ConclusionThere is high quality evidence to suggest thatH. pylorieradication therapy leads to both cure and improvement in FD symptoms, although the benefit is modest.
Selective arterial temporary flow arrest with balloons during transvenous embolization for the treatment of brain arteriovenous malformations: a feasibility study with MRI-monitored adverse events
Authors: Iosif Christina , Almeida Filho Jose Alberto , Gilbert Clara Esther , Nazemi Rafie Ali , Saleme Suzana , Rouchaud Aymeric , Mounayer Charbel 
Published: January 2022

BackgroundThe technique of endovascular transvenous embolization for brain arteriovenous malformations (AVMs) has emerged in the last 8 years as a very promising therapeutic alternative for otherwise incurable cases. Selective temporary flow arrest during transvenous endovascular embolization (TFATVE) is a novel adaptation of our previously described transvenous approach, which employs hyper-compliant balloons intra-arterially for the selective occlusion of arterial feeders during ethylene vinyl copolymer (EVOH) injection, in order to reduce intra-nidal pressure and increase nidi occlusion rates.MethodsWe performed a feasibility study of the TFATVE technique between January 2016 and April 2020. Consecutive patients were included. All patients had at least one axial brain MRI or CT in the first 48 hours following intervention, and at least one brain MRI scan within the first postoperative month, in order to detect both silent and clinically evident adverse events. Patients’ demographics, angio-architectural characteristics, total injection and procedure times, angiographic and clinical outcomes were analyzed.Results22 patients underwent TFATVE during transvenous endovascular treatment of brain AVMs. Among them, 86.4% were high Spetzler-Martin’s grade. Good clinical outcome (modified Rankin Scale <2) was achieved in 95.5% of the cases, with 0% of procedure-related mortality and 4.5% of clinically significant, procedure-related morbidity. Total occlusion of the nidus was achieved in >90% of the cases at the end of the procedure and angiographic stability was achieved in all cases; 100% of the cases had angiographic cure at follow-up.ConclusionsTFATVE seems a safe and effective technique when conducted in carefully selected patients in highly specialized centers.