bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fibulin-3 sponges Tiam1 to manipulate MMP-7 activity through β-catenin signaling in oral squamous cell carcinoma
Authors: Guo Honglei , Ji Yangyang , Zhang Bo , Huang Xin 
Published: April 2022

Abstract
Purpose: Oral squamous cell carcinoma (named OSCC) is considered as the most frequent malignancy in oral cavity, which has became a rapid increasing problem for the global public health with unclear underling molecular mechanism. Previously, we reported Tiam1 (T-lymphoma invasion and metastasis inducing factor 1) as a potential oncogene for OSCC. Here, we in-depth explored its signaling mechanism. Methods: The mRNA and protein expression levels of primary differentially expressed genes (Tiam1, Fibulin-3 and MMP-7) were measured in different TNM stages of OSCC patients using RT-PCR and ELISA respectively. Using human OSCC cell line CAL27 cell line, the relationships between these factors have been analyzed and the direct interaction has been also examined. The luciferase reporter assay was established for the promoter region of MMP-7. Both the epithelial (E-cadherin) and mesenchymal protein markers (Vimentin and Snail) have been investigated using western blotting. Results: The mRNA and protein activities of Fibulin-3 declined as the increase of TNM stage. Inversely, the mRNA and protein levels of Tiam1 and MMP-7 elevated significantly as OSCC progressed. Tiam1 transfection in CAL27 cells stimulated the expression of MMP-7 by accelerating the nuclear translocation of β-catenin, which was opposite to the functions of Fibulin-3. Moreover, Tiam1 interacted directly with Fibulin-3. The Tiam1 induced OSCC epithelial-mesenchymal transition (EMT) via MMP-7 activation, which was dependent of β-catenin direct binding on the promoter region. Conclusions: Collectively, these results indicated that Tiam1 competed with Fibulin-3 for nuclear β-catenin translocation, which subsequently stimulated MMP-7 expression by TCF-4 domain interaction following EMT initiation in OSCC development. Our systematical work here hypothesized an innovative signaling cassette for OSCC progression, which provided beneficial references for future clinical study.
Metastasis of lung adenocarcinoma within a pituitaryneuroendocrine tumor: case report and review of the literature
Authors: Suzuki Koji , Tahara Shigeyuki , Hattori Yujiro , Teramoto Shinichiro , Ishisaka Eitaro , Inomoto Chie , Osamura Robert Yoshiyuki , Morita Akio 
Published: April 2022

Abstract
Introduction: Collision tumors in which malignant neoplasms metastasize to pituitary neuroendocrine tumors (PitNETs) are extremely rare. We report a case of PitNET with a history of colorectal and bladder cancers and a relatively rapid progression of neurological symptoms.Case Report: A 75-year-old man who underwent tumor resection for bladder cancer 36 years before and for colon cancer 18 years before without recurrence suffered from right-sided homonymous hemianopsia, ptosis and diplopia of the right eye. MRI revealed a tumor with a diameter of 3.2 cm, extending from the anterior pituitary gland to the suprasellar region. Gadolinium-enhanced MRI of the tumor showed heterogeneous contrast enhancement. Semi-emergency endoscopic endonasal transsphenoidal surgery was performed because of the relatively rapid progression of neurological symptoms. Histopathological examination revealed a group of thyroid transcription factor-1- and napsin A-positive papillary proliferating cells intermingled with α-subunit (α-SU)- and steroidogenic factor-1-positive PitNET cells. The patient was diagnosed with metastasis of lung adenocarcinoma within a PitNET (gonadotroph PitNET).Results: Genetic testing revealed a positive EGFR (Ex-19del) mutation, and chemotherapy was initiated. Additional stereotactic radiotherapy was performed for the residual tumor in the sella turcica. Chemotherapy was continued, and 24 months later, both the primary and metastatic tumors were well controlled.Conclusion: Cases of metastasis of a malignant neoplasm within a PitNET are difficult to diagnose. In the case of a sella turcica tumor with relatively rapid progression of neurological symptoms, early surgical intervention is recommended because of the possibility of a highly proliferative tumor and to obtain pathologic specimens.
A qualitative study of Influences, Sources of Information and Media Consumption habits on Uptake of Contraceptive Services among people of Reproductive age in Uganda
Authors: Namanda Cissie , Atuyambe Lynn , SSali Sarah , Mukose Aggrey , Tumwesigye Nazarius Mbona , Makumbi Frederick E. , Tweheyo Ritah , Gidudu Andrew , Sekimpi Carole , Hashim Catherine Verde , Nicholson Martha , Ddungu Peter 
Published: April 2022

Abstract
Background
Uganda has registered an increased investment in family planning (FP) programmes, which has contributed to improvement in knowledge of modern contraceptive methods being nearly universal. However, this has not matched uptake of modern methods and reduction in unmet need of FP. Due to these challenges, a programme on improving awareness, access to and uptake of modern contraceptives is being implemented. We therefore conducted a formative study to determine the influences, key sources and media consumption habits of contraceptive information at the onset of this programme.
Methods
Using a qualitative study design, we conducted thirty-two focus group discussion and twenty-one in-depth interviews involving men and women of reproductive age. We also carried out twenty-one key informant interviews with people involved in FP service delivery. Data was collected in 4 districts where implementation of the program was to take place. Audio recorders were used to collect data and tools translated in local languages. A codebook was developed, transcripts coded in vivo using a computer software Atlas-ti version 7 before analysis. Ethical clearance was obtained from institutional review boards and informed consent sought from all participants.
Results
Three themes emerged from the data; influences to contraceptive uptake, sources of contraceptive information and media consumption habits. From the study, most married people with children mentioned health workers as influences while adolescents reported their peers and friends to be their main influence. Religious leaders and mothers in law were reported to mainly discourage people from taking up modern contraceptive methods. Key sources of information were health workers followed by radio. Television, mobile vans mounted with megaphones were other sources of information. Radio was majorly among male whereas television was a source common among female living in urban settings. For media consumption habits, participants advocated for messages in a language free of vulgar words that would interest men. In conclusion, health workers, religious leaders and mothers determine uptake of contraceptive services. The study recommends the consideration of the role of these influences at design of FP program interventions as well as more involvement of health workers in sensitization of communities about contraceptive methods.
Evaluation Of Intestinal Permeability With Plasma Zonulin Level In Patients With Vitiligo And Its Correlation With Inflammatory Cytokines And Oxidative Stress Markers
Authors: Arslan Selami , Altunisik Nihal , Turkmen Dursun , Uremis Muhammed Mehdi , Sener Serpil , Turkoz Yusuf 
Published: April 2022

Abstract
Background: It has been proven that there is an increase in intestinal permeability in some autoimmune diseases. In our study, we aimed to evaluate intestinal permeability in vitiligo disease by looking at zonulin levels. At the same time, we aimed to examine the correlation of inflammatory cytokines, oxidative stress markers and lipopolysaccharide (LPS) levels with zonulin.Methods: Fourty one vitiligo patients and 41 healthy volunteers were included in our study. Venous blood samples were taken from the patient and control groups, and the levels of zonulin, LPS, Interleukin (IL)-6, Tumor necrosis factor (TNF)-α and oxidative stress markers were examined.Results: The zonulin mean of the patient group was found to be statistically significantly higher than the control group (p<0.05). A positive and statistically significant correlation was found between zonulin level and IL-6, TNF-α and LPS levels (p<0.05). While the TNF-α and LPS levels of the patient group were found to be significantly higher than the control group, there was no significant difference in terms of IL-6 levels. The reduced glutathione, nitric oxide, total antioxidant status levels in the erythrocytes were lower and malondialdehyde levels were higher in the patient group compared to the control group. Conclusion: We think that serum zonulin level increases and intestinal permeability increases in vitiligo disease.
Dynamic Programming of the Stochastic Burgers Equation Driven by Levy Noise
Authors: MOHAN MANIL T. , SAKTHIVEL K. , SRITHA SIVAGURU. S 
Published: April 2022

Abstract
Abstract. In this work, we study the optimal control of stochastic Burgers equation perturbed by Gaussian and L´evy type noises with distributed control process acting on the state equation. We use the dynamic programming approach for the second order Hamilton-JacobiBellman (HJB) equation consisting of an integro-differential operator with L´evy measure associated with the stochastic control problem. Using the regularizing properties of the transition semigroup corresponding to the stochastic Burgers equation and compactness arguments, we solve the HJB equation and the resultant feedback control problem.
Retinometer as a postoperative predictor of visual acuity in patients with senile cataract
Authors: Paez Ivan , Macias Jaime , Lansingh Van Charles , Eckert Kristen A. 
Published: April 2022

Abstract
Objective: To evaluate the estimation of postoperative visual acuity (VA) by a retinometer and its correlation with cataract characteristics in patients undergoing cataract surgery. Material and Methods: A prospective case series was conducted on a consecutive sample of patients with senile cataract who underwent cataract surgery from September through December 2021. Before surgery, the retinometer estimated postoperative best-corrected VA (BCVA). Estimations were compared to postoperative BCVA 1 month after surgery. Logistic regression evaluated the correlation between estimations and cataract characteristics [cataract grade (color and opacity) and density].Results: Among 64 patients (64 eyes) who underwent cataract surgery, 28 (43.8%) had equivalent postoperative BCVA estimations and real values, 36 (56.2%) showed better BCVA than predicted, and none showed lower postoperative BCVA than predicted. Postoperative outcomes were accurately predicted in 55% of mild, 55.6% of moderate, and 11.8% of severe cataract cases. Cataract grade and density were significant predictors of postoperative BCVA (R 0.69, P < 0.0001).Conclusion: Retinometer accuracy varied according to cataract severity. Results support the preoperative use of the retinometer in mild and moderate cataract cases as a useful tool to inform patients of their visual prognosis following surgery.
The small RNA STnc1480 contributes to the regulation of biofilm formation and pathogenicity in Salmonella Typhimurium
Authors: li jing , Ning Chengcheng , Li Na , Guo Yun , Ji Chunhui , Zhu Xiaozhen , Zhang Xingxing , Meng Qingling , Xia Xianzhu , Cai Xuepeng , Qiao Jun 
Published: April 2022

Abstract
Salmonella Typhimurium (STM) is one of the most important food-borne bacteria that seriously harm livestock and human beings, which is capable of regulating the expression of its own genes in a variety of ways to adapt to a wide variety of adverse environmental stresses. To understand the regulatory roles of sRNA STnc1480 on the capability of STM, the STnc1480 gene-deficient strain △STnc1480 and its complement strain △STnc1480/STnc1480 were generated, and the impacts of STnc1480 gene deficiency on the capability of responding to different environmental stresses, BF formation and pathogenicity were analyzed, respectively. Then, the target genes that were regulated by STnc1480 were also analyzed and explored. Compared with parent and complement strains, the deficiency of the STnc1480 gene significantly reduced the BF formation. Moreover, the capacities of adhesion and invasiveness of the △STnc1480 strain to macrophages were also significantly reduced, while the LD50 in mice was significantly increased. The bacterial loads in liver and spleen were significantly reduced, and the pathological damage was alleviated. It was confirmed that the STnc1480 could be complementary to the 5’-UTR (-52 to -71 bases) region of lpfA mRNA. The bacterial dual plasmid reporting system confirmed that STnc1480 was capable of interacting with the mRNA of the lpfA gene, suggesting that STnc1480 can regulate the 5’-UTR of the lpfA mRNA at post transcription level to reduce the expression of the bacterial fimbria, thus reducing the BF formation and pathogenicity of STM.
A Novel Biomarker Selection Method Combining Graph Neural Network and Gene Relationships Applied to Microarray Data
Authors: Li Wei , Xie Weidong , Zhang Shoujia , Wang Linjie , Yang Jinzhu , Zhao Dazhe 
Published: April 2022

Abstract
Background: The discovery of critical biomarkers is significant for clinical diagnosis, drug research and development. Researchers usually obtain biomarkers from microarray data, which comes from the dimensional curse. Feature selection in machine learning is usually used to solve this problem. However, most methods do not fully consider feature dependence, especially the real pathway relationship of genes. Results: Experimental results show that the proposed method is superior to classical algorithms and advanced methods in feature number and accuracy, and the selected features have more significance. Method: This paper proposes a feature selection method based on a graph neural network. A graph structure and real feature dependence combined with Pearson correlation coefficient is used in our method to construct graph structure model, the information propagation and aggregation method of graph neural network is used to characterize node information, and the spectral clustering method is used to filter redundant features. Then, the feature ranking aggregation model using eight feature evaluation methods acts on each clustering sub-cluster for different feature selection. Conclusion: The proposed method can effectively remove redundant features. The algorithm’s output has high stability and classification accuracy, which can potentially select potential biomarkers.
A preliminary exploration of pancreatic ferroptosis: Inhibition of ferroptosis alleviates pancreatic injury through the NLRP3 pathway in acute pancreatitis
Authors: Li Jie , Cheng Long , Ji Liang , Tang De-sheng , Yan Chang-sheng , Guo Xiao-yu , Cui Ji-tao , Cao Feng , Wang Gang 
Published: April 2022

Abstract
Objective The purpose of this study was to explore whether ferroptosis exists in acute pancreatitis (AP) and the effect of iron ions on the NLRP3 pathway.Materials and Methods A total of 45 male C57BL/6 mice were randomly divided into three groups (control, AP, AP+2, 2'-bipyridyl). A total of 12 injections (caerulein, 50 μg/kg) were given at one-hour intervals. The mice in the AP+2, 2'-bipyridyl group were pretreated with 2, 2'-bipyridyl (20 mg/kg) for 1 hour and then injected with caerulein. The mice in the control group were injected with an equal volume of normal saline. All of the mice were killed one hour after the last injection. The pancreases were harvested for histopathological evaluation, immunohistochemistry analyses and western blotting. One-way ANOVA and Student’s t test were performed. ﻿Results The ferroptosis inhibitor 2, 2'-bipyridyl can prevent the accumulation of iron ions, reduce the formation of lipid peroxides and alleviate injury in the process of AP, and reduce pancreatic inflammation through the NLRP3 pathway.Conclusion This experiment revealed the presence of ferroptosis in AP. The application of 2, 2'-bipyridyl can obviously alleviate pancreatic damage through the NLRP3 pathway.
Machine learning algorithms to automate differentiating cardiac amyloidosis from hypertrophic cardiomyopathy
Authors: Wu Ziwen , Zheng Jin-Lei , Kuang Lin , Yan Hui 
Published: April 2022

Abstract
Background: Cardiac amyloidosis has poor prognosis, high mortality and is often misdiagnosed as hypertrophic cardiomyopathy, leading to delayed diagnosis. Objective: Machine learning combined speckle tracking echocardiography was proposed to automate differentiating two conditions.Methods: A total of 74 patients with pathologically confirmed cardiac amyloidosis and 64 patients of hypertrophic cardiomyopathy were enrolled from June 2015 to November 2018. Machine learning models utilizing traditional and advanced algorithms were established and determined the most significant predictors. The performance was evaluated by receiver operating characteristic curve (ROC) and area under the curve (AUC). Results: With clinical and echocardiography data, all models showed great discriminative performance (AUC > 0.9). Compared with logistic regression (AUC=0.91), machine learning such as support vector machine (AUC=0.95, P=0.477), random forest (AUC= 0.97, P=0.301) and gradient boosting machine (AUC= 0.98, P=0.230) demonstrated varying degrees of improvement of predicting cardiac amyloidosis. With speckle tracking echocardiography, the predictive performance of the voting model was similar to that of LightGBM (AUC were 0.86 for both), while the AUC of XGBoost was slightly lower (AUC=0.84). In 5-fold cross validation, the voting model was more robust globally and superior to the single model in some test sets. Conclusions: Data-driven machine learning had shown admirable performance in differentiating two conditions and could automatically integrate abundant variables to identify the most discriminating predictors without making preassumptions. In the era of big data, automated machine learning will help to better identify patients with cardiac amyloidosis, and to timely and effectively intervene, thus improving the outcome.
New spermatological characteristics in the Hemiuroidea inferred from the ultrastructure of the spermatozoon of Gonocerca phycidis a stomach parasite of Gephyroberyx darwinii (Teleostei: Trachichthyidae) off the Senegal coast
Authors: Bâ Aïssatou , Bakhoum Abdoulaye J.S. , Ndiaye Papa Ibnou , Bray Rodney Alan , Bâ Cheikh Tidiane , Marchand Bernard , Quilichini Yann 
Published: April 2022

Abstract
The present study provides the first ultrastructural data on the digenean Gonocerca phycidis belonging to the family Gonocercidae. Live adults digenean were collected from the stomach of the marine fish Gephyroberyx darwinii (Teleostei: Trachichthyidae) caught in the Atlantic Ocean, off Dakar (Senegal). This is also the first report of G. phycidis from Senegal and this host species. The mature spermatozoon exhibits (i) two axonemes with the 9 + ‘‘1’’ pattern of the Trepaxonemata, (ii) a lateral expansion associated with external ornamentations and without cortical microtubules, (iii) two types of external ornamentation of the plasma membrane located in the anterior extremity of the spermatozoon, (iv) two bundles of cortical microtubules in the mitochondrial and nuclear areas of the spermatozoon, (v) one mitochondrion and (vi) a nucleus. The maximum number of cortical microtubules is located in the median part of the spermatozoon. Moreover, the mature spermatozoon of G. phycidis presents some peculiarities. The presence of a lateral expansion without cortical microtubules associated with external ornamentations is described here, for the first time, in digenean spermatozoa. The presence of two elongated bundles of cortical microtubules is also reported for the first time in the superfamily of Hemiuroidea. However, the ultrastructure of the posterior spermatozoon extremity confirms that already described in other hemiuroidean species.
Iso-Graph: Its Definition and Properties
Authors: Song Yuqing 
Published: April 2022

Abstract
This paper introduces a discrete Reeb graph model, called iso-graph, on arbitrary graphs, and discusses its properties. We define a sect on a graph as an edge subset which, if removed, transects the input graph, changing it homotopically. We show that an iso-graph is essentially a collection of sects which are nested and not inversely tangent. The iso-graph model can be used as a substitute for existing discrete Reeb graphs with the advantages of avoiding data perturbation and interpolation.
Learning Domain-Specific Edit Operations from Model Repositories with Frequent Subgraph Mining
Authors: Tinnes Christof , Kehrer Timo , Joblin Mitchell , Hohenstein Uwe , Biesdorf Andreas , Apel Sven 
Published: April 2022

Abstract
Model transformations play a fundamental role in model-driven software development. They can be used to solve or support central tasks, such as creating models, handling model co-evolution, and model merging. In the past, various (semi-)automatic approaches have been proposed to derive model transformations from meta-models or from examples. These approaches require time-consuming handcrafting or the recording of concrete examples, or they are unable to derive complex transformations. We propose a novel unsupervised approach, called Ockham, which is able to learn edit operations from model histories in model repositories. Ockham is based on the idea that meaningful domain-specific edit operations are the ones that compress the model differences. It employs frequent sub-graph mining to discover frequent structures in model difference graphs. Learning Domain-Specific Edit Operations We evaluate our approach in two controlled experiments and one real-world case study of a large-scale industrial model-driven architecture project in the railway domain. We found that our approach is able to discover frequent edit operations that have actually been applied before. Furthermore, Ockham is able to extract edit operations that are meaningful to practitioners in an industrial setting. We also discuss some of the use cases for the discovered edit operations in this industrial setting.
Compound heterozygous p.L483P and p.S310G mutations in GBA1 cause type 1 adult Gaucher disease: case report with a novel mutation
Authors: Wen Xiaoling , Wang Yaozi , Zhang Xialin , Zhang Zhijuan , Liu Xiaxia , Lu Haiyan , Hao Guoping , Wang Xiaohuan , Yang Linhua , Zhang Ruijuan 
Published: April 2022

Abstract
Background
Gaucher disease (GD) is a rare autosomal recessive hereditary lysosomal storage disease. It is caused by glucocerebrosidase (GBA1) gene mutation that leads to decreased activity of acid β-glucosidase (glucocerebrosidase [GCase]). GD genotype-phenotype correlation is not fully understood. The aim of the current study was to identify and characterize compound heterozygous mutations of GBA1 in a type 1 adult GD patient.
Results
Using enzymological studies and whole exome sequencing, the patient with type 1 GD was found to becompound heterozygous for GBA1 missense mutations in exon 10 c.1448T > C (p.L483P) and exon 7 c.928A > G (p.S310G). The patient's mother who had Parkinson disease history was heterozygous for p.S310G mutation. The asymptomatic father was heterozygous for p.L483P mutation. The sister did not carry either mutation. Structural modeling showed both p.S310G and p.L483P are far away from the GCase active site. The p.S310G mutation in domain 1 could cause decreased stability between the α2 and α3 helices of GBA1. The p.L483P mutation in domain 2 could reduce the van der Waals force of the side chain and disrupt the C-terminal -sheet.
Conclusions
The p.L483P/p.S310G novel compound heterozygous mutation underlies type 1 GD and likely impacts GCase protein function. This is the first description of p.S310G associated with mild type 1 GD in the context of coinherited p.L483P mutation.