bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of gelatin drying methods on its amphiphilicity
Authors: Voroshilin Roman , Kurbanova Marina , Ostapova Elena , Makhambetov Eduard , Petrov Andrey , Khelef Mohammed El Amine 
Published: September 2022

Gelatin is a natural amphiphilic biopolymer that is widely used in food products, pharmaceuticals, and cosmetics. We studied the effect of spray and freeze drying on the solubility and amphiphilicity of gelatin samples.
The control sample was a commercially produced edible gelatin. The experimental samples were spray- and freeze-dried gelatins obtained by enzymatic-acid hydrolysis of cattle bone. Amino acid sequences were determined by matrix-activated laser desorption/ionization. Solubility was assessed visually. Bloom strength of the gelatin gels was measured by a texture analyzer. The ProtScale online service was used to predict the amphiphilic topology of gelatin proteins. Molecular weight distribution of proteins was carried out by electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate.
Spray drying reduced protein degradation and retained more α-chains, while freeze drying increased gelatin’s hydrophobicity and decreased its solubility. The predicted topology of protein hydrophobicity based on the amino acid sequences was in line with our results on solubility. The freeze-dried gelatin had a 18% larger amount of low-molecular weight peptides, compared to the control and the spray-dried samples. This was probably caused by the cleavage of peptides during the drying process. Thus, freeze drying can lead to maximum degradation of gelatin components, which may be associated with a longer heat treatment, compared to spray drying.
Thus, spray drying is more suitable for gelatin, since this method improves the stability of its outer and inner structure, ensuring high hydrophilic properties.
Development of plant-based yogurt
Authors: Baskar Nehaa , Varadharajan Sabitha , Rameshbabu Mathushree , Ayyasamy Sudha , Velusamy Sangeetha 
Published: September 2022

Fermented drinks are regarded as healthy food due to their probiotic nature. Vegan consumers who choose sustainable diet and people allergic to dairy products demand alternatives for dairy products. We aimed to develop a non-dairy plant-based yogurt from peanut, oats, and coconut milk.
Yogurt was formulated with addition of sugar, corn starch, pectin, and xanthan gum. Simplex-lattice mixture design was applied to optimize the composition of the yogurt and achieve the desired rheological properties, sensory attributes, and syneresis rate.
Our results revealed that the formulation containing 7.13 mL of peanut milk, 10 mL of oats milk, and 7.86 mL of coconut milk showed low syneresis rate, desired viscosity and flow behavior, as well as high overall acceptability. We found that increased amounts of peanut and oats milk improved the product’s viscosity due to high protein contents. However, coconut milk enhanced the taste and flavor of the yogurt. Flow behavior depended on viscosity and stabilizers used in accordance with the power law model. Syneresis rate was influenced by the viscosity of the yogurt. The utilization of corn starch, pectin, and xanthan gum not only improved the texture but also helped achieve the desired viscosity and flow behavior.
The nutrient composition, physicochemical properties, and high sensory characteristics of the yogurt based on peanut, oats, and coconut milk allow using it as a cow milk alterative in the diet of people with lactose intolerance.
A comparative study of phytochemical, antioxidant, anticarcinogenic, and antidiabetic potential of coriander (Coriandrum sativum L.): Microgreen and mature plant
Authors: Dhakshayani G. M. , Priya S. Janaki Alias 
Published: September 2022

Microgreens are immature edible leafy greens with a higher concentration of phytonutrients than in mature leaves, which makes them a novel functional food. This research featured antioxidant, anticarcinogenic, and antidiabetic properties of coriander microgreens.
Aqueous and ethanolic extractions of coriander microgreens and mature leaves underwent a phytochemical analysis of antioxidant potential using the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method and the ferric reducing antioxidant power (FRAP) assay. The analysis of antidiabetic and anticarcinogenic properties included the method of α-amylase enzyme inhibition and the MTT colorimetric assay.
The screening test inferred the presence of alkaloids, terpenoids, glycosides, steroids, tannins, flavonoids, phenols, carbohydrates, and proteins in both microgreens and mature leaves. The quantitative analysis showed that the ethanolic extract of the microgreen sample exhibited higher total phenols. Total flavonoids, steroids, carbohydrates, and proteins were higher both in microgreen extracts, if compared with those of mature leaves. Ascorbic acid, chlorophyll-a, chlorophyll-b, and carotenoids demonstrated a more substantial presence in mature leaves. The gas chromatography-mass spectrometry (GC/MS) analysis of coriander microgreens revealed such bioactive compounds as thienopyrimidines, phenolic amide, imidazo pyridazine, phenolic constituents, and essential oil. Mature leaves were rich in phenolic compounds, steroids, terpenoids, essential oils, and fatty acid esters. All these substances are known for their therapeutic antioxidant, antidiabetic, and anticarcinogenic properties. The microgreen samples exhibited greater ferric reducing antioxidant power, α-amylase enzyme inhibition, and cytotoxicity activity at a lower concentration of extract than mature leaves.
Coriander microgreens proved to have a promising antioxidant, anticarcinogenic, and antidiabetic potential and can be used in daily food additives.
Carboxymethyl cellulose and psyllium husk in gluten-free pasta
Authors: Faheid Siham M.M. , Rizk Ibrahim R.S. , Kishk Yasser F.M. , Ragab Gamal H. , Mostafa Sayed 
Published: September 2022

Formulating high-quality pasta from wheat-free materials is a technological challenge. We aimed to make gluten-free pasta with carboxymethyl cellulose and psyllium husk and evaluate their effect on the quality of the final product.
Gluten-free pasta was produced from rice flour, white corn flour, potato starch, soy protein isolate, and carboxymethyl cellulose or psyllium husk used as binding agents. Then, we evaluated the effect of these hydrocolloids on the color, texture, cooking quality, and sensory characteristics of the product.
The uncooked gluten-free pasta containing psyllium husk showed significantly higher values of hardness compared to the samples with carboxymethyl cellulose, while the cooked pasta with psyllium husk had a significantly lower nitrogen loss. Also, psyllium husk improved the texture of the cooked gluten-free pasta, providing the highest values of resilience, springiness, and chewiness. Generally, the psyllium husk samples received higher quality values for texture, cooking quality, and sensory parameters, compared to the pasta with carboxymethyl cellulose.
Psyllium husk showed a better ability to bind gluten-free pasta than carboxymethyl cellulose. Consequently, psyllium husk could become a feasible alternative to wheat gluten in producing high-quality gluten-free pasta.
Effects of bioactive substances isolated from Siberian medicinal plants on the lifespan of Caenorhabditis elegans
Authors: Faskhutdinova Elizaveta , Sukhikh Andrey , Le Violeta , Minina Varvara , Khelef Mohammed El Amine , Loseva Anna 
Published: September 2022

Medicinal plants are sources of natural antioxidants. Acting as reducing agents, these substances protect the human body against oxidative stress and slow down the aging process. We aimed to study the effects of bioactive substances isolated from medicinal plants on the lifespan of Caenorhabditis elegans L. used as a model organism.
High-performance liquid chromatography was applied to isolate bioactive substances from the extracts of callus, suspension, and root cultures of meadowsweet (Filipendula ulmaria L.), ginkgo (Ginkgo biloba L.), Baikal skullcap (Scutellaria baicalensis L.), red clover (Trifolium pretense L.), alfalfa (Medicágo sativa L.), and thyme (Thymus vulgaris L.). Their effect on the lifespan of C. elegans nematodes was determined by counting live nematodes treated with their concentrations of 10, 50, 100, and 200 µmol/L after 61 days of the experiment. The results were recorded using IR spectrometry.
The isolated bioactive substances were at least 95% pure. We found that the studied concentrations of trans-cinnamic acid, baicalin, rutin, ursolic acid, and magniferin did not significantly increase the lifespan of the nematodes. Naringenin increased their lifespan by an average of 27.3% during days 8–26. Chlorogenic acid at a concentration of 100 µmol/L increased the lifespan of C. elegans by 27.7%. Ginkgo-based kaempferol and quercetin, as well as red clover-based biochanin A at the concentrations of 200, 10, and 100 µmol/L, respectively, increased the lifespan of the nematodes by 30.6, 41.9, and 45.2%, respectively.
The bioactive substances produced from callus, root, and suspension cultures of the above medicinal plants had a positive effect on the lifespan of C. elegans nematodes. This confirms their geroprotective properties and allows them to be used as anti-aging agents.
Yogurt-like product from lupine (Lupinus albus L.) milk as an alternative to dairy products
Authors: Kavas Nazan 
Published: September 2022

Recently, the number of people suffering from allergy to caw’s milk has increased. Lupine, a plant rich in protein, can be a good alternative product for non-dairy products production. We aimed to obtain a yogurt-like product based on lupine milk and evaluate its properties.
Lupine milk was obtained from lupine seeds, egg white protein powder, disaccharides, and starter cultures were added to the milk to obtain yogurt-like products: samples with maltose, samples with lactose, and samples without sugars. Physico-chemical and microbiological characteristics of the products were determined by the standard techniques. Sensory attributes were evaluated by trained panelists.
In the study, the effects of egg white protein powder and disaccharides on the activities of starter cultures and the properties of the yogurt-like products obtained were investigated. The relationship between the addition of sugar and the growth of starter cultures was found to be significant (P < 0.05). In terms of physico-chemical, rheological, and microbiological properties, the yogurt-like products obtained from lupine milk with disaccharides demonstrated good results, especially the sample with maltose. Sensory analysis revealed high sensory properties of the yogurt-like products.
Yogurt-like products from lupine milk can be used as an alternative to cow’s milk fermented products, but more detailed studies should be conducted on their formulations.
Positive Predictive Value of Computed Tomography Virtual Laryngoscopy in Detecting Laryngeal Carcinoma Taking Histopathology as Gold Standard
Authors: Rashid Nawaz , Noreen Aliya , Dogar Iqbal Hussain , Mansoor Ali , Hashmi Kamran Sajjad , Nawaz Fareeha 
Published: September 2022

Background: Computed tomography virtual laryngoscopy (CTVL) is favored diagnostic tool in detecting laryngeal carcinoma because it delivers excellent temporal and spatial resolution. Objective: To determine the positive predictive value of computed tomography virtual laryngoscopy in detecting laryngeal carcinoma taking histopathology as gold standard. Methods: A cross sectional survey was conducted at department of Radiology, Mayo Hospital Lahore. The completion of study was done in six months [Feb 16, 2019 till Aug 16, 2019]. After obtaining approval from ethical committee and informed consent from subjects, 64 subjects were included in the study who presented in the Department of the Radiology having carcinoma of larynx on CTVL and then sent to department of ENT of mayo hospital, where they underwent direct laryngoscopy and afterwards biopsy obtained sent to the department of histopathology. All the subjects undergoing CT scan examination on the CT machine of hospital. Contrast injection was given to all subjects undergoing CT scan, through power injector at the rate of 4ml per min and dosage of 0.1ml/kg. Same machine was used to conduct all CT scans and was inferred through same radiologist and all the histopathology was performed by same pathologist to exclude bias. Results: The mean age of patients was 44.38 ± 9.57 years with minimum and maximum age as 22 and 60 years. There were 47(73.4%) male and 17(26.6%) female cases. On histopathology the laryngeal carcinoma was diagnosed in 56(87.5%) of the cases while it was negative in 8(12.5%) of the cases. On CT the laryngeal carcinoma was diagnosed in 59(92.2%) of the cases while it was negative in 5(7.8%) of the cases. The Positive Predictive Value of CT was 89.83%, while its sensitivity was 94.64%, specificity was 25.00%, negative predictive value was 40.00% and diagnostic accuracy was 85.94%. Conclusion: Through the findings of this study it is concluded that positive predictive value of computed tomography virtual laryngoscopy is high (89.83%) in detecting laryngeal carcinoma taking histopathology as gold standard. CT is a precise and non-invasive imaging method in detecting laryngeal carcinoma.
Validity of Pediatric Emergency Care Applied Research Network (PECARN) in Pediatric Trauma Patients - A Cross Sectional Study from a Tertiary Care Hospital in Pakistan
Authors: Naseer Naureen , Kapadia Nazir Najeeb , Masud Sarwat 
Published: September 2022

Introduction: Head trauma in the pediatric age group is a common presentation in the Emergency Room (ER). The
Pediatric Emergency Care Applied Research Network (PECARN) tool can help an ER clinician to identify pediatric head
trauma patients at very low risk of clinically important traumatic brain injuries (ciTBIs) and avoid unnecessary CT scans.
Objective: To determine the validity of PECARN rule as compared to the head CT (gold standard) in identifying low risk
traumatic brain injury pediatric patients. Methods: A cross-sectional study was done at Aga Khan University Hospital (AKUH) Emergency Department ED. All pediatric head trauma patients were included in this study between 2017-2019. A total of 218 head trauma cases were reviewed which were evaluated for the PECARN criteria. Data were extracted on a prepared data collection form. Data were entered and analyzed using SPSS. Chi-square test was used. Results: Among the total 218, 190 cases (87%) had CT scans ordered. Out of these 190 patients 156 (82%) met PECARN criteria, while 34 (18%) were PECARN negative. The sensitivity and specificity of PECARN was calculated as 82% and 33% respectively, with a fair level of agreement with CT scan based on Kappa statistics. Conclusion: PECARN has a higher sensitivity but lower specificity in comparison to CT scan.
Assessment of Parental Satisfaction with Day-Care Surgical Services in a Tertiary Level Pediatric Hospital
Authors: Ahmed Riaz , Akhtar Jamshed , Zamir Naima , Taqvi Syed Muhammad Raees Hussain , Bibi Safia 
Published: September 2022

Background: Parental feedback is important for improving quality of the surgical services provided.Objective: This study was conducted to assess the level of satisfaction of parents with the day-care surgical services so as to find out where improvement is needed. Methods: This was an observational cross sectional study conducted in the Department of Pediatric Surgery, National Institute of Child Health, Jinnah Sindh Medical University Karachi from September 2019 to April 2020. The parents of the children who underwent different day-care surgical procedures were included. A validated Parent Satisfaction Scale (PSS) which consist of 11-items was used. The responses were made according to Likert scale. Three groups of satisfied, undecided and unsatisfied study participants were made according to the scores obtained. The socio-demographic variables including age of parents and children, level of education, and income group were compared amongst study groups. Results: Total of 114 parents participated. The most common procedure performed was inguinal herniotomy (n=59). Seven (6%) patients were brought back after discharge due to pain. Eighty-seven (76.30%) parents were satisfied with the services provided while 18 (15.8%) were unsatisfied and 9 (7.9%) undecided. A significant difference in the satisfaction level of the study participants noted with respect to the mean age of parents (p=0.039), level of education (p< 0.001) and income groups (p< 0.001). Conclusion: Majority of the study participants were satisfied with the surgical services provided. Lack of respect given by the hospital staff and delay in getting treatment were the main reasons for dissatisfaction. Socio-demographic characteristics of the study participants were significantly associated with the level of satisfaction.
Pregnancy Outcome in Acute Viral Hepatitis E
Authors: Sultana Rashida , Humayun Shamsa , Manzoor Sofia , Humayun Sara 
Published: September 2022

Background: Hepatitis E is associated with poor feto-maternal outcome. Patients with acute hepatitis E who are pregnant usually require hospital admission and monitoring with liver function tests and clotting profile. Objective: To evaluate maternal and fetal outcome among pregnant women who have positive serology for hepatitis E IgM. Methods: Data of women with clinical presentation of jaundice during pregnancy and who were diagnosed as acute hepatitis E was collected on pre-structured questionnaire after obtaining consent. Data were entered in Statistical Package for Social Sciences for analysis. Results: Three hundred and thirty three women had positive serology for hepatitis E IgM. Out of these three hundred were in last trimester of their pregnancy. The gestational age of two hundred and thirty two (77%) women was less than 37 weeks at presentation. Six (2%) pregnancies ended up in miscarriage, fifty four (16 %) had fetal demise in maternal womb while two hundred and seventy three (82%) were viable fetus. One third of live born babies were kept in NICU. Indications of neonatal admission were respiratory distress in 57(72%), jaundice neonatorum in 12(15%), asphyxia neonatorum in 9(12%), while one (1%) had transient tachypnea. Neonates who died in first week of their life were 18(23%).The cause of neonatal death was respiratory distress in 12 (67%) and anoxia in remaining 6 (33%) neonates. Most of the women were discharged home however maternal mortality was recorded in 80(24%) patients, mainly in postpartum period 73(91%). Conclusion: Pregnant women with acute hepatitis E have more complicated course of this viral infection with adverse fetal and maternal implications.
Mental health in arsenic-induced cancer: a study from middle Gangetic plain of Bihar, India
Authors: Kumar Shishu Kesh , Ghosh Ashok , Bharti Das Ambika 
Published: September 2022

AbstractThe health impact of groundwater arsenic contamination shall remain partially understood while its psychological aspects are not studied. So, this correlational survey research explored mental health as a function of perceived social support in arsenic-induced cancer (AIC), which is highly prevalent in the middle Gangetic plain. Mental health inventory and multidimensional scale of perceived social support were used to assess mental health (MH) and perceived social support (PSS) respectively in 77 AIC patients. Findings show significant impact of PSS on MH in the arsenic-affected regions. PSS from significant others have strong impact on overall MH. Perception of reality was predicted by PSS from family while global PSS predicted two dimensions of MH, i.e. positive self-evaluation and environmental mastery. This baseline study suggests that global PSS and that from family and significant others is important and has a strong impact on MH of this population. These findings would help early identification and/or psychological intervention (from non-government organizations, oncology social workers/researchers, and policy makers) to promote better health and well-being in this arsenic-affected population.
Knowledge, Attitude and Practice of the use of Nanoparticles in the Treatment
of Oral Cancer Patients
Authors: Mohanty Neeta , Mohapatra Diksha , Rath Shakti 
Published: September 2022

Aims:
To determine the knowledge, attitude and practice of the use of nanoparticles amongst health care professionals for treating oral cancer patients. Also, to understand the indications and contraindications of nanoparticles in treating oral cancer patients.

Methods:
A descriptive cross-sectional study was executed amongst dental surgeons practising with undergraduate and post-graduate degrees. A Google questionnaire form was developed and distributed, and 102 authentic entries were short-listed. Data obtained from the study were recorded in Microsoft Excel 2007 version, and SPSS version 18.0 was used. Calculation of statistical analysis like mean and standard deviation and analysis between variations was done using student t-tests, and the association between variables was determined using Chi-square. A value was found significant when the p-value was less than 0.05.

Result:
Many practitioners have been using nanoparticles prepared by using nanobiotechnology for the last 2-4 years. 92.9% of participants are well conversant with nanoparticles in treating oral cancer patients, out of which 88% are willing to specify therapeutic medicaments prepared using nano-biotechnology for oral cancer patients. 94.9% of the dentists are accepting to the fact that nanomedicine can also be used in combination therapy for treating patients, and 98% stated that the use of nanotechnology for diagnosing as well as treating patients will not only be advantageous but will also be the new face of cancer management shortly.

Conclusion:
Lack of awareness, increased tobacco consumption, and late diagnosis are the prime concerns about increasing oral cancer cases and decreased prognosis. Nanoparticles having a small particulate size will be able to detect the changes on the surface and the cellular levels and inside the cells. Similarly, in the case of therapeutic use, nanoparticles are highly efficient in delivering drugs to the systemic circulation by reducing the drug’s immunogenicity by releasing the drug in an environmentally responsive dose, thus lowering the frequency of administration and systemic toxicity.
In vitro and in vivo anti-lung cancer activity of emodin: A RNA-seq transcriptome analysis
Authors: Maimaiti Aikebaier , Xingliang Liu , Shi Linsen 
Published: September 2022

Abstract:
Emodin is a natural anthraquinone extract of Chinese medicinal herbs. Several studies demonstrated the antitumor activity of this extract, particularly for lung cancer. However, the mode of action of emodin remains obscure. In this study we evaluated in vitro and in vivo anti-lung cancer properties of emodin in human lung adenocarcinomas and the underlying molecular mechanism involved. Our results demonstrated emodin caused a significant inhibition of cell viability and induced apoptosis in lung cancer cells to a level that is relative to that of normal epithelial cells. Furthermore, RNA-seq transcriptome analysis revealed 65 common genes(>2-fold change) with a significant expression in A549, and H1650 cells under emodin treatment. In vitro qRT-PCR results confirmed that 4 genes (ATP6V0D2, C11orf96, TIPARP, and CDKN1A) were up-regulated, and 9 genes (TNFSF10, IFIT1, EGLN3, RCOR2, ARRB1, FLVCR2, BAIAP2-DT, FAM111B, and TGFB2) were down-regulated in A549 and H1650 adenocarcinoma cell lines. The in vivo experiment demonstrated emodin could significantly decrease tumor volume and tumor weight of mice compared with the control group. However, emodin treatment has no obvious effect on body weight of mice. Moreover, in vivo qRT-PCR results revealed that 3 genes (ATP6V0D2, C11orf96, and TIPARP) were up-regulated, and 6 genes (TNFSF10, IFIT1, EGLN3, BAIAP2-DT, FAM111B, and TGFB2) were down-regulated in tumor tissues compared with the control group. These findings suggested that emodin could help treat lung cancer and has a potential for further investigations as an anti-lung cancer drug.