bluuebunny/crossref_metadata_2025_split huggingface dataset:
LAG3-PD1 or CTLA4-PD1 Inhibition in Advanced Melanoma: Indirect Cross Comparisons of the CheckMate-067 and RELATIVITY-047 Trials
Authors: Nie Run-Cong , Zhang Fei-Yang , Wang Yun , Chen Guo-Ming , Nie Man , Zhao Zhou-Kai , Chen Xiao-Jiang , Jiang Kai-Ming , Yuan Shu-Qiang , Chen Ying-Bo 
Published: June 2022

Abstract
OBJECTIVE To compare the inhibition of lymphocyte-activation gene 3 (LAG3) plus programmed cell death 1 (PD-1) versus the inhibition of cytotoxic T-lymphocyte antigen 4 (CTLA-4) plus PD-1 in patients with previously untreated advanced melanoma.DESIGN A meta-analysis of individual participant data (IPD).DATA SOURCES PubMed, Web of Science and Embase were searched from January 1, 2015, to January 14, 2022, for eligible studies.ELIGIBILITY CRITERIA FOR STUDY SELECTION Data from randomized controlled trials reporting on the use of dual LAG3-PD1 inhibitors or dual CTLA4-PD1 inhibitors which assigned PD1 inhibitor alone as their control arm and provided corresponding Kaplan-Meier (KM) curves of treatment outcomes for previously untreated advanced melanoma were retrieved. METHODS The IPD data were extracted from the KM plots using a graphical reconstructive algorithm. Log-rank, Cox proportional hazard model, Bayesian hierarchical model with time-varying hazard ratio (HR) effect, and restricted mean survival time (RMST) were performed to estimate survival benefits. The primary endpoint was progression-free survival.RESULTS The CheckMate-067 (N = 630) and RELATIVITY-047 (N = 714) trials were included for analysis. The graphical reconstructive algorithm showed that IPD had similar HRs and log-rank values as the original plots. The HR of nivolumab-relatlimab (LAG3 inhibitor) versus nivolumab-ipilimumab (CTLA4 inhibitor) was 1.19 (95% confidence interval [CI] 0.96 to1.48). The 24-months RMST of nivolumab-relatlimab versus nivolumab alone was 2.35 (95% CI 0.77 to 3.94) months, compared with 1.87 (95% CI 0.25-3.49) months for nivolumab-ipilimumab versus nivolumab. The Bayesian hierarchical model showed that patients treated with nivolumab-relatlimab had earlier PFS benefits than those with nivolumab-ipilimumab. Grade 3 or 4 treatment-related adverse events occurred in 18.9% of patients using nivolumab-relatlimab and 55.0% of patients using nivolumab-ipilimumab.CONCLUSION These findings suggest that the PFS of LAG3-PD1 and CTLA4-PD1 inhibition were similar and that LAG3-PD1 inhibition tended to exhibit earlier survival benefit and lesser TRAEs.
A multi-wavelength observations on the infrared dust bubble N75
Authors: Cui Quanling , Zhang Chuan-Peng , Wang Jun-Jie 
Published: June 2022

Abstract
We presented a study on the infrared dust bubble N75 using the IRAM 30m telescope at Spain and searched for star formation evidence in its surroundings. We observed C18O, HCO+, HNC, N2H+ towards N75 and performed a multiwavelength study around G38.93 with data taken from large-scale surveys: GLIMPSE, MIPSGAL, GRS, NVSS and BGPS. We analyzed the spectral profifile and the distribution of the molecular gas and dust in the G38.93. Morphologically, the C18O emission correlates well with Spitzer IRAC 8.0µm emission. The channel map and the velocity-position diagram show that the outflflow exists in G38.93. We calculated the physical parameters of the outflflow including the mass, size, column density and optical depth. We found only several YSOs coincident with the bright bubble rims. It is possible that YSOs have not yet formed, or will not be, formed in the bubbles shells, or they are simply hidden amid the high IR background. The dynamical age (∼ 5.1×105 yr) of outflflow is close to the expanding age (∼ 2.5 × 105 yr) of the bubble. It could be that the both are formed at the same evolutionary period.
Kernel Method for Estimating Matusita Overlapping Coefficient Using Numerical Approximations
Authors: Omar  , Enas  
Published: June 2022

Abstract
In this paper, the nonparametric kernel method is used to estimate the well known overlapping coefficient known as Matusita 𝜌. Due to the complexity of finding the formula expression of this coefficient when using the kernel estimators, we suggest to use the numerical integration method to approximate its integral as a first step. Then the kernel estimators were combined with the new approximation to formulate the proposed estimators. Two numerical integration rules known as trapezoidal and Simpson rules were used to approximate the interesting integral. The proposed technique produced two new estimators for 𝜌. The proposed estimators are studied and compared with existing estimator developed by Eidous and Al-Talafheh (2020) via Monte-Carlo simulation technique. The simulation results clearly demonstrated the usefulness and efficiency of the new technique for estimating 𝜌.
Dermato-kit: A newly developed dotted lateral flow immunochromatographic kit for rapid diagnosis of dermatophytosis
Authors: Aboul-Ella Hassan , Hamed Rafik , Abo-Elyazeed Heidy 
Published: June 2022

Abstract
Dermatophytosis is a widely spread contagious zoonotic disease, affecting both man (tinea) and animals (ringworm). This disease is caused by a group of closely related keratinophilic fungi known collectively as the dermatophytes group. Although the wide distribution of dermatophytosis cases throughout the whole world and its adverse clinical effect on human health, economical effect on productive animals, and pet animal welfare, there is no rapid accurate diagnostic tool for such disease. Up to date, dermatophytosis diagnosis is limited to direct, rapid with low sensitivity and specificity several wet mounts techniques, sophisticated with high skilled personnel requirement molecular assays, and gold standard with long time consumption conventional culturing and biochemical testing. All of the previously mentioned existing diagnostic methods are lab confined techniques with no availability of a field, handheld, or bedside tests. The different aspects of difficulties facing dermatophytosis diagnosis lead to mistaken diagnosis and faulty prescription of antifungals with its expected long list of side effects on man and animals. The current conducted study tries to accomplish the difficult equation by achieving an accurate, sensitive, specific, user-friendly, rapid, robust, device less, deliverable to end-users, and economic cost for development and production diagnostic kit. The newly designated and developed kit, based on lateral flow immune-chromatographic assay, showed a very promising competitive diagnostic result.
Nucleophilic Attack Enables Chemical Suture of Crystalline Silicon at Room Temperature
Authors: Deng Xuebiao , Zeng Tao , Li Jun , Yao Guoying , Chen Huai , Xu Liangpang , Wei Linfeng , Shakouri Mohsen , Yu Jimmy , Wang Ying , Yang Zhenyu 
Published: June 2022

Abstract
Crystalline silicon (c-Si) has been widely used in semiconductor and energy-related industries. One of the biggest challenges of c-Si production is its high energy and environmental cost due to the requirement of high-temperature synthetic process and the accompanied intense greenhouse gas emission. Herein, we demonstrate a new process for preparing c-Si directly from hydride-terminated silicane (HSi) under mild conditions. We design a wet-chemistry approach that effectively creates Si-Si bonds between HSi flakes via nucleophilic attack by Lewis base reagent at room temperature. c-Si produced by such a chemical suturing process demonstrates promising capability in charge carrier migration and separation under visible light irradiation, which is of critical importance in photocatalysis. Compared to the existent c-Si manufacturing processes, the reported approach drastically reduces the energy consumption and provides a new strategy to tailor the electronic and photophysical properties of c-Si for optoelectronic and catalytic applications.
Predictive Analytics for Cold Chain Break Detection
Authors: Kale Swati Dilip 
Published: June 2022

Abstract
Perishable food deteriorates at any step in the cold chain due to various internal and external factors. The leading cause of deterioration is fluctuating ambient temperatures. An intelligent cold chain management system comprises proper observation and control of perishable food parameters from food safety. The current work develops a predictive model that considers critical factors like vehicle characteristics, initial and environmental temperature, method of loading and unloading the food in-vehicle, and measurement of Mean Kinetic Temperature (MKT). Predictive data analytics is carried out using machine learning algorithms for real-time forecasting of food quality at each stage of the cold chain. Machine learning models are a step forward in cold chain management that is more accurate and requires less computing time than traditional techniques such as CFD (Computational Fluid Dynamics) method, Kriging method, and capacitive heat transfer method. Thus, the developed system will avoid cold chain breakage caused by temperature abuse during transit. Experimentation with various perishable foods is being considered. The results are experimentally validated in real-time applications.
Microbiological quality of ready-to-eat vegetable salads served at meat restaurants under the COVID 19 pandemic induced food demand: an example from Eastern Turkey
Authors: Baran Alper , Adigüzel Mehmet Cemal , Aydin Hakan 
Published: June 2022

Abstract
Ready-to-eat (RTE) salads service and utilize with pide/lahmacun, kebab types, and tantuni in meat restaurants in Turkish cuisine. Hence, the vegetable salads served with meat-based RTE foods are a risk factor for public health. In this study, we report the microbiological properties, antimicrobial resistance of foodborne pathogens including E. coli and S. aureus, and the presence of important enteric viruses in eastern Turkey. The total aerobic bacteria (TAB) ranged from < 1 to 6.40 log CFU g− 1 in the RTE salads (n = 42), whereas yeast and molds was ranged from < 1 to 6.26 log CFU g− 1. The coliform bacteria and the Staphylococcus and Micrococcus spp. count were determined < 1-5.82 log CFU g− 1 and < 1-5.66 log CFU g− 1 in all REVS samples, respectively. None of the RTE vegetable salads (REVS) tested in this study contained Salmonella spp., whereas E. coli and S. aureus were isolated in 38.1% (16/42) and 2.4% (1/42), respectively. S. aureus was resistant to gentamicin, kanamycin, aztreonam, and ciprofloxacin in the disc diffusion assay, however, it was not harboring the mecA gene. E. coli strains (n = 16) were resistant (100%) to aminoglycoside antibiotics (gentamicin and kanamycin) and 35.7% (6/16) of the isolates were ESBL producing. BlaTEM and blaCTXM8/25 were detected in two isolates, whereas one isolate carried blaCTXM−1 and blaTEM together by PCR. Of the REVS, two were evaluated as positive for rotavirus (4.8%), and six for hepatitis A (14.3%) and hepatitis E virus (14.3%). These results indicate the high microorganism load, presence of ESBL E. coli, and viral enteric pathogens in REVS, hence it is important to perform routine hygiene practices.
Molecular Surveillance of Shiga Toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria
Authors: Ayoade Femi , Oguzie Judith , Akano Kazeem , Olumade Testimony , Eromon Philomena , Odesola Kanyinsola , Folarin Onikepe , Happi Christian 
Published: June 2022

Abstract
Shiga toxigenic strains of E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/ occurrence in selected abattoirs and retail meat sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design. Samples were plated directly on selective and differential media and confirmed serologically using latex agglutination serotyping kit, then, multiplex PCR analysis was used to screen for the presence of specific virulence factors. The results showed a percent occurrence of STEC at the sampled sites ranging from 25.8- 46.3%. None of the strains showed any visible agglutination with the O157 latex reagent. Sequence analysis of PCR products was performed on a representative isolate showing the highest combination of virulence genes. This sequence was subsequently submitted to GenBank with accession number MW463885. The sequence showed 100% coverage and 96.46% percentage identity with Escherichia coli O113:H21 (GenBank Accession number: CP031892.1) strain from Canada. From evolutionary analyses, the strain from Nigeria, sequenced in this study, is evolutionarily distant when compared with the publicly available sequences from Nigeria. Although no case of E. coli O157 was found within the study area, percent occurrence of non-O157 STEC as high as 46.3% at some of the sampled sites is worrisome and requires regulatory interventions in ensuring hygienic practices at the abattoirs within the study area.
Teilrevision Raumplanungsgesetz – notwendige demokratische Legitimation für Biomasseanlagen in der Landwirtschaftszone
Authors: Schibli Beatrix 
Published: June 2022

Der Ständerat befasst sich in dieser Sommersession unter anderem mit der Teilrevision des Raumplanungsgesetzes, 2. Etappe («Teilrevision RPG 2»). In diesem Rahmen wird er auch über eine Änderung der raumplanungsrechtlichen Rahmenbedingungen für Biomasseanlagen in der Landwirtschaftszone beraten. Der vorliegende Beitrag zeigt auf, dass die vorgeschlagene Gesetzesbestimmung im Hinblick auf mehr Rechtssicherheit und höherer demokratischer Legitimation verbessert werden müsste.
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Durant cette session d'été, le Conseil des Etats se penche entre autres sur la révision partielle de la loi sur l'aménagement du territoire, 2e étape (« Teilrevision RPG 2 »). Dans ce cadre, il discutera également d'une modification des règles d’aménagement du territoire pour les installations de biomasse en zone agricole. Cet article montre que la disposition légale proposée devrait être améliorée en vue d'une plus grande sécurité juridique et d'une plus grande légitimité démocratique.
Efektivitas Pemberian Minuman Isotonik dan Jeruk Manis Pacitan Peras (Citrus Sinensis) terhadap Vo2 Max Atlet Sepak Bola
Authors: Adhimah Ayu Fauziyyah , Harun Iriyanti , Winiastri Dinda 
Published: June 2022

AbstractThe purpose of this study was to analyze the difference effect of sport drink and sweet orange juice on VO2 max value in soccer athletes. This study was in the field of quasi experimental design with pre-post test with controlled group. The subject of this study were 22 soccer athletes which are calculated by Lameshow formula. The subject has given 300 ml sport drink, sweet orange juice without any additional ingredients, and placebo for 3 days intervention. The VO2 max value was measured by Balke running test before dan after the treatment. Data were analyzed by paired sample t-test, one way ANOVA, dan post-hoc LSD. Based on the results of the paired sample t-test, there were significant differences between the values before dan after treatment (p0.05). There was an increase of VO2 max in the sweet orange group (5.53±3.09) dan sport drink group (3.2±2.11). There wasn’t a significant difference effectivity of sport drink (52.68±2.27) ml/kgBB/minute dan sweet orange juice (54.86±1.29) ml/kgBB/minute on VO2 max value in football athletes (p0.05). Sport drink and sweet juice orange can increase VO2 max in football athletes. There is no difference in the effectiveness of VO2 max in both of these drinks. AbstrakPenelitian ini bertujuan untuk mengetahui ada tidaknya perbedaan efektivitas pemberian minuman isotonik dan jeruk manis peras terhadap VO2 max atlet sepak bola. Desain penelitian ini adalah quasi experimental, pre-post test with control group design. Jumlah subjek penelitian sebanyak 22 atlet sepak bola yang dihitung berdasarkan rumus Lameshow. Subjek penelitian pada 3 kelompok berbeda menerima 3 hari intervensi pemberian 300 ml isotonik bermerek, jeruk manis peras, dan placebo. Nilai VO2 max diukur dengan menggunakan tes Balke. Nilai VO2 max dianalisis menggunakan uji paired sample t-test, uji one way ANOVA, dan dilanjutkan dengan uji Post-hoc LSD. Berdasarkan hasil uji paired sample t-test, terdapat perbedaan yang signifikan antara nilai sebelum dan sesudah perlakuan pada kedua kelompok (p0,05). Terjadi peningkatan VO2 max pada kelompok jeruk manis peras (5,53±3,09) dan kelompok minuman isotonik (3,2±2,11) ml/kg BB/menit. Tidak terdapat perbedaan signifikan efektivitas minuman isotonik (52,68±2,27) ml/kg BB/menit dan jeruk manis peras (54,86±1,29) ml/kg BB/menit terhadap VO2 max atlet sepak bola (p0,05). Rerata VO2 max sebelum perlakuan ketiga kelompok relatif sama atau tidak ada perbedaan yang signifikan (p0,05), sedangkan rerata VO2 max sesudah perlakuan ketiga kelompok mempunyai perbedaan yang signifikan (p 0,05). Pemberian minuman isotonik dan jeruk manis peras dapat meningkatkan VO2 max atlet sepak bola, dan tidak terdapat perbedaan efektivitas di antara keduanya.
The Impact of Migration Processes on Economic Security and Space Development of Russia
Authors: Arkhipova L. S. , Gorokhova I. V. 
Published: June 2022

The topicality of researching specific features of migration processes on the territory of the Russian Federation deals with the rising role of economic security in providing strategic priorities of states in the period of growing tension in the world. Apart from that, such factors as unfavorable demographic situation, changes in the age structure of the population, adverse trends on labour market of many regions, shortage of labour, etc. become more and more important. It increases academic and practical interest in researching migration processes on different territorial levels, including the sphere of national and economic security. The Decree of the President of the Russian Federation N 208 dated May 13, 2017 ‘Concerning Strategy of Economic Security in the Russian Federation for the Period up to 2030’ defined optimization of labour migration flows based on needs of national economy as one of key objectives of realizing the trend dealing with the development of state governance system, forecasting and strategic planning in economy. This document among indicators characterizing economic security focuses on the tension factor on labour market, the share of employable people in the total population and distribution of those employed by the level of education. The Degree of the President of the Russian Federation N 400 dated July 2, 2021 ‘Concerning Strategy of National Security of the Russian Federation’ points out that attaining goals of economic security of the Russian Federation is realized by solution of such important problems as cutting differentiation of entities of the Russian Federation by the level and rate of social and economic development, standard of living, motivating the development of economic potential in regions, strengthening of their budget support. Migration processes are included in the zone of such problem solution. They can cause even higher territorial differentiation in regions, a drop in the standard of life or, on the contrary, reduce territorial inequality and improve space development of the country. In view of national and economic security external migration is more important, as it influences many social and economic aspects taking place in the country. But whether migration processes inside the country can affect economic security of Russia is still a debatable issue.
In Transfusion-Dependent Thalassemia Children, Increased Iron
Overload is Associated with Lower Serum Alpha-Klotho, Which is
Strongly Associated with Lower Total and Ionized Calcium
Concentrations
Authors: Moustafa Shatha Rouf , Al-Hakeim Hussein Kadhem , Alhillawi Zainab Hussein , Maes Michael 
Published: June 2022

Background:
Patients with transfusion-dependent thalassemia (TDT) show
disorders in calcium metabolism. The α-Klotho protein is predominantly expressed in
tissues that are involved in calcium homeostasis, and lowered levels are associated with
bone disease. The aim of the study is to examine the associations between low α-Klotho
status and calcium metabolism in relation to iron status in children with TDT.

Methods:
Calcium, α-Klotho, parathyroid hormone (PTH), calcyphosin, vitamin D3,
phosphorous, fibroblast growth factor receptor 2 (FGFR2), as well as iron and erythron
biomarkers were measured in 60 children with TDT and 30 healthy control children.

Results:
A meaningful part of TDT patients showed lowered α-Klotho levels, and those
children also showed low serum total and ionized calcium concentrations. TDT patients
showed increased PTH, FGFR2, and calcyphosin and lowered vitamin D3 as compared
with healthy children. The α-Klotho levels were significantly correlated with total and
ionized calcium (positively) and with iron overload and transfusions biomarkers
(inversely). Partial Least Squares path analysis showed that 40.1% of the variance in
serum total calcium could be explained by the regression on α-Klotho, vitamin D3 (both
positively), and calcyphosin (inversely) and that the effects of the latter are mediated by
iron overload and the number of blood transfusions.

Conclusion:
In conclusion, the iron overload in TDT and its consequences may induce
lowered levels of α-Klotho which in turn may lead to lower calcium thereby explaining at
least in part the effects of TDT on bone metabolism including spontaneous pathological
fractures, osteoporosis, osteopenia, and skeletal deformities.
Application of Induced Pluripotent Stem Cells in Moyamoya Disease:
Progress and Promises
Authors: Xue Yimeng , Zhang Qian , Wang Lin-Jian , Tu Wen-Jun , Zhao Jizong 
Published: June 2022

Abstract:
Moyamoya disease (MMD) is a chronic steno-occlusion cerebrovascular disease accompanied
by the formation of the abnormal vascular network at the base of the brain. The etiology of MMD is not
fully clarified. Lack of pathological specimens hinders the research progress. Induced pluripotent stem
cells (iPSC) derived from patients with outstanding differentiation potential and infinite proliferation
ability could conquer the problem of insufficient samples. The technology of iPSC holds the promise of
clarifying the underlying molecular mechanism in the development of MMD. In this review, we summarized
the latest progress and difficulties in the research of mechanism and detailed the application of iPSC
in MMD, aiming to provide an outlook of iPSC in molecular mechanism and novel therapies of MMD.
A Novel Compound Heterozygous Gene Mutation of Dolichol Kinase Deficiency
(DOLK-CDG)
Authors: Wang Caixia , Yu Shufeng , Zhang Ying , Chen Zhihong , Song Jiye 
Published: June 2022

Background:
Congenital disorder of glycosylation caused by mutation of the
DOLK(DOLK-CDG) is a group of rare autosomal recessive diseases with an early-onset age and
poor prognosis. DOLK-CDG can cause the dysfunction of multiple systems and organs such as
the heart, skin, nerves, and bones.

Case Presentation:
We report a child with DOLK-CDG diagnosed and treated in the Affiliated
Hospital of Qingdao University. The child was born with neonatal asphyxia, Ichthyoid rash, and
congenital heart disease. His fingers of both the hands looked like lotus roots, and the palm and
foot were covered by a white membrane. He was hospitalized with a severe infection at 4 months
after birth. Physical examination showed that he was complicated with development delay and
hypotonia. He experienced convulsions 1 hour after admission and died of multiple organ failure
2 hours after admission. Blood samples were taken for genetic testing before the child died. The
results showed that there was a novel compound heterozygous mutation in DOLK, c.1268C＞G
(P.P423R）and c.1581_1583del (P.527_528del).

Conclusion:
This mutation is new and not included in the human gene mutation library. The
discovery of the novel mutation broadened the mutation spectrum of DOLK. At the same time,
we sorted out the DOLK-CDG gene mutation sites and related clinical manifestations reported
by August 2021 through a literature review.