bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts
Authors: Villányi Vanda , Gondor Orsolya Kinga , Bánfalvi Zsófia 
Published: November 2022

Abstract
Introduction
Earliness of tuberisation and the quality of potato tubers are important traits in potato breeding. The qualitative traits rely on the metabolite profile of tubers, which are storage organs and net importers of assimilates. Thus, the quality of tubers largely depends on the metabolites transported from leaves to developing tubers.

Objectives
To test the influence of canopy on the quality of tubers by metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts.

Methods
Potatoes were grown under greenhouse conditions, grafted and the tubers harvested at the end of the scions’ vegetation period. Metabolite profiling of freshly harvested tubers was performed using gas chromatography coupled with mass spectrometry. Statistical analyses were applied to determine the significant differences between the different tubers.

Results
99 metabolites were identified and an additional 181 peaks detected in chromatograms, out of which 186 were polar and 94 non-polar compounds. The concentrations of 113 metabolites were significantly different in the tubers from the early-maturing CE3130 and the late-maturing CE3027 line. Hetero-grafting resulted in considerable changes in the metabolite content of tubers. Especially, the effect of CE3027 on the metabolite composition of tubers formed on CE3130 rootstocks was readily apparent. Nevertheless, many compounds were present at similar levels in the tubers of hetero-grafted plants as was found in the tubers of their scion counterparts.

Conclusion
Hetero-grafting resulted in many compounds at similar concentrations in rootstock tubers as in scion tubers suggesting that these are transported from the source leaves to tubers.
A note on the hierarchical multi-switch multi-echelon vehicle routing problem
Authors: Tadaros M. , Migdalas A. , Samuelsson B. 
Published: November 2022

AbstractThis paper introduces the Hierarchical Multi-Switch Multi-Echelon Vehicle Routing Problem, a new variant of the well-known Vehicle Routing Problem. It is a real-world problem originating from the policies of a Nordic distribution company. The problem includes a single depot, a non-predetermined hierarchy of intermediate facilities, and two different fleets, consisting of homogeneous original and homogeneous local vehicles, which are pulling swap-bodies. Original vehicles with attached swap-bodies depart from the central depot. They can either visit customers directly if only one swap-body is attached or visit one or two consecutive switch points in order to transfer one or two loaded swap-bodies to a corresponding number of local vehicles, which are subsequently routed to customers while the original vehicle itself proceeds to serve customers with the remaining loaded swap-body. A mixed-integer formulation of the problem is proposed. A short bibliographic review, relations, shared characteristics, and differences of the proposed variant and several known VRP variants are analyzed and discussed. The solution of an illustrative instance is presented in order to demonstrate the solution concept for the problem as well as to compare with solution concepts for previously stated VRP variants. Computational experiments on small instances that could be solved within one hour are also presented. The problem is computationally hard to solve. Thus, the development of heuristics and metaheuristics is an important future task in order to enable solution of real case instances or instances of realistic sizes.
A Comparative Study of High Temperature Tensile and Creep Testing Between Standard and Miniature Specimens: Applicability and Limits
Authors: Tang Yuanbo T. , Schwalbe Caspar , Futoma Magdalena , Roebuck Bryan , Utada Satoshi , Reed Roger C. 
Published: November 2022

AbstractThis study concerns the quasi-static and time-dependent mechanical behavior obtainedviathe miniaturized electro-thermal mechanical testing (ETMT) approach for single crystal (SX) and conventional cast Mar-M-247 superalloy. The experimental outcome was benchmarked against standardized testing procedures. It is found that tensile yielding behavior can be captured accurately by the ETMT approach up to 1100$$^{\circ }$$∘C, provided the appropriate type of thermocouple (T/C) is chosen. Furthermore, creep rupture behavior is underestimated by the miniaturized set-up. High repeatability of the rupture time was obtained for the SX case, whereas a significant scatter was observed for the conventional cast case. The discrepancies are assessed in detail; discussion centers around analytical and practical considerations, such as temperature uncertainty due to parasitic voltage and the choice of T/C, microstructural change as a result of the Joule heating, representative gauge volume, and strain rate non-linearity. Consequently, the applicability and limits of the miniaturized approach are examined critically, and improvements were suggested where appropriate.
Effects of Peripheral Neural Blocks in Laparoscopic Sleeve Gastrectomy: a Pilot Study on Cognitive Functions in Severe Obese Patients
Authors: Zhao Xinyang , Xue Qi , Dong Ling , Chu Zhaoxia , Wang Yong , Chen Chanjuan , Hu Xianwen , Zhang Ye , Huang Chunxia 
Published: November 2022

Abstract

Background
In addition to the analgesic effect, peripheral neural blocks also prevent cognitive impairment and peripheral inflammation induced by surgery. However, it is unknown if there is collateral impact on cognitive improvement after bariatric surgery.

Methods
In this pilot study, 75 patients with severe obesity for selective laparoscopic sleeve gastrectomy (LSG) were recruited and randomized into three groups (1:1:1) as general anesthesia (GA) group, transverse abdominis plane block (TAPB) group, and quadratus lumborum block (QLB) group. Bilateral TAPB or QLB was performed (0.33% ropivacaine with dexmedetomidine 1 μg/kg) before the standardized general anesthesia. Cognitive test battery was completed before LSG and in 1-month and 3-month follow-up. The levels of peripheral inflammatory cytokines were determined at equivalent time points.

Results
Patients with LSG exhibited massive cognitive improvement in postoperative 3 month without or with TAPB or QLB (Ptime < 0.001). Compared to GA, QLB significantly strengthened performance in MoCA (β = 0.56, 95%CI: 0.08, 1.05). IL-6, IL-8, and high-sensitivity CRP significantly verified among three groups. Changes in IL-6 within postoperative 3 months were negatively correlated with MMSE and MoCA, and positively correlated with AVLT-DR for QLB group. Similar correlation was found in the GA group for changes in IL-6 and AVLT-IR.

Conclusion
Laparoscopic sleeve gastrectomy ideally improved memory and attention as early as postoperative 1 month. QLB promoted cognitive improvement in MoCA, which was negatively correlated with changes in IL-6. More precise trials are needed to determine the overall effect of peripheral neural block on cognition following bariatric surgery.

Graphical Abstract
Normalization of Cardiac Function After Bariatric Surgery Is Related to Autonomic Function and Vitamin D
Authors: Snelder Sanne M. , Aga Yaar , de Groot - de Laat Lotte E. , Biter L. Ulas , Cabezas Manuel Castro , Pouw Nadine , Birnie Erwin , Boxma - de Klerk Bianca , Klaassen René A. , Zijlstra Felix , van Dalen Bas M. 
Published: November 2022

Abstract
Purpose
Subclinical cardiac dysfunction is common in patients with obesity. Bariatric surgery is associated with normalization of subclinical cardiac function in 50% of the patients with obesity. The aim of this study was to identify predictors for a lack of improvement of subclinical cardiac dysfunction 1-year post-bariatric surgery.

Methods
Patients who were referred for bariatric surgery were enrolled in a longitudinal study. Inclusion criteria were age 35–65 years and BMI ≥ 35 kg/m2. Patients with a suspicion of or known cardiovascular disease were excluded. Conventional and advanced echocardiography, Holter monitoring, and blood tests were performed pre- and 1-year post-bariatric surgery. Subclinical cardiac dysfunction was defined as either a reduced left ventricular ejection fraction, decreased global longitudinal strain (GLS), diastolic dysfunction, arrhythmia, or an increased BNP or hs Troponin I.

Results
A total of 99 patients were included of whom 59 patients had cardiac dysfunction at baseline. Seventy-two patients completed the 1-year follow-up after bariatric surgery. There was a significant reduction in weight and cardiovascular risk factors. Parameters of cardiac function, such as GLS, improved. However, in 20 patients cardiac dysfunction persisted. Multivariate analysis identified a decreased heart rate variability (which is a measure of autonomic function), and a decreased vitamin D pre-surgery as predictors for subclinical cardiac dysfunction after bariatric surgery.

Conclusion
Although there was an overall improvement of cardiac function 1-year post-bariatric surgery, autonomic dysfunction and a decreased vitamin D pre-bariatric surgery were predictors for a lack of improvement of subclinical cardiac dysfunction.

Graphical abstract
Catalytic reduction of water pollutants: knowledge gaps, lessons learned, and new opportunities
Authors: Liu Jinyong , Gao Jinyu 
Published: November 2022

AbstractIn this paper, we discuss the previous advances, current challenges, and future opportunities for the research of catalytic reduction of water pollutants. We present five case studies on the development of palladium-based catalysts for nitrate, chlorate, and perchlorate reduction with hydrogen gas under ambient conditions. We emphasize the realization of new functionalities through the screening and design of catalytic metal sites, including (i) platinum group metal (PGM) nanoparticles, (ii) the secondary metals for improving the reaction rate and product selectivity of nitrate reduction, (iii) oxygen-atom-transfer metal oxides for chlorate and perchlorate reduction, and (iv) ligand-enhanced coordination complexes for substantial activity enhancement. We also highlight the facile catalyst preparation approach that brought significant convenience to catalyst optimization. Based on our own studies, we then discuss directions of the catalyst research effort that are not immediately necessary or desirable, including (1) systematic study on the downstream aspects of under-developed catalysts, (2) random integration with hot concepts without a clear rationale, and (3) excessive and decorative experiments. We further address some general concerns regarding using H2 and PGMs in the catalytic system. Finally, we recommend future catalyst development in both “fundamental” and “applied” aspects. The purpose of this perspective is to remove major misconceptions about reductive catalysis research and bring back significant innovations for both scientific advancements and engineering applications to benefit environmental protection.
Artificial Nerve Containing Stem Cells, Vascularity and Scaffold; Review of Our Studies
Authors: Kakinoki Ryosuke , Akagi Masao 
Published: November 2022

AbstractTo promote nerve regeneration within a conduit (tubulation), we have performed studies using a tube model based on four important concepts for tissue engineering: vascularity, growth factors, cells, and scaffolds. A nerve conduit containing a blood vascular pedicle (vessel-containing tube) accelerated axon regeneration and increased the axon regeneration distance; however, it did not increase the number or diameter of the axons that regenerated within the tube. A vessel-containing tube with bone-marrow-derived mesenchymal stem cell (BMSC) transplantation led to the increase in the number and diameter of regenerated axons. Intratubularly transplanted decellularized allogenic nerve basal lamellae (DABLs) worked as a frame to maintain the fibrin matrix structure containing neurochemical factors and to anchor the transplanted stem cells within the tube. For the clinical application of nerve conduits, they should exhibit capillary permeability, biodegradability, and flexibility. Nerbridge® (Toyobo Co. Ltd., Osaka, Japan) is a commercially available artificial nerve conduit. The outer cylinder is a polyglycolic acid (PGA) fiber mesh and possesses capillary permeability. We used the outer cylinder of Nerbridge as a nerve conduit. A 20-mm sciatic nerve deficit was bridged by the PGA mesh tube containing DABLs and BMSCs, and the resulting nerve regeneration was compared with that obtained through a 20-mm autologous nerve graft. A neve-regeneration rate of about 70%–80% was obtained in 20-mm-long autologous nerve autografts using the new conduits.
Graphical Abstract
Best of ASCO 2022—central nervous system tumors
Authors: Leibetseder Annette , Berghoff Anna S. 
Published: November 2022

SummaryIn this article, updates on novel therapy approaches in central nervous system tumors presented at the ASCO 2022 meeting are summarized. Promising outcome results on targeted therapies in rare entities such as neurotrophic tyrosine receptor kinase (NTRK) fusion-positive tumors [1] and in v‑RAF murine sarcoma viral oncogene homolog (BRAF)V600 mutant pediatric glioma are reported [2, 3]. Furthermore, we shortly review data on additional administration of polyADP-ribose polymerase (PARP) inhibitor veliparib (Alliance A071102 trial) [4] and on a combinatorial immunotherapy regime (consisting of intramuscular administration of two synthetic DNA plasmids in combination with cemiplimab) in newly diagnosed glioblastoma patients [5].
Teacher Feedback, Student ADHD Behavior, and the Teacher–Student Relationship: Are These Related?
Authors: Staff Anouck I. , Oosterlaan Jaap , van der Oord Saskia , de Swart Fanny , Imeraj Lindita , van den Hoofdakker Barbara J. , Luman Marjolein 
Published: November 2022

AbstractThis cross-sectional study aimed to compare positive and corrective teacher feedback toward children with attention-deficit/hyperactivity disorder (ADHD) and typically developing children, and to examine whether the quality of the teacher–student relationship moderated associations between child behavior and teacher feedback. We observed and coded classroom ADHD behaviors of 55 children with ADHD and 34 typically developing children (TDC; 6–12 years), as well as the levels of positive and corrective teacher feedback they received. Teachers rated closeness and conflict using the Student–Teacher Relationship Scale in the ADHD group. Multilevel analyses revealed that teachers provided significantly more corrective feedback to children in the ADHD compared to the TDC group. Children in the ADHD group received more corrective than positive feedback, but this pattern was reversed for the TDC group. Multiple regression analyses in the ADHD group indicated that lower levels of positive feedback were related to higher levels of motor hyperactivity. Higher levels of corrective feedback were associated with higher levels of verbal hyperactivity. Closeness moderated this association: Corrective feedback was related to levels of verbal hyperactivity only if teachers experienced less closeness in the relationship with the child. None of the other moderation effects were significant. Teachers provided more corrective feedback to children with ADHD than to typically developing children, and teacher feedback toward children with ADHD was associated with levels of hyperactivity. A close teacher–student relationship may serve as protective factor for the receipt of corrective feedback in this group, but experimental studies are needed to confirm this hypothesis.
Melatonin controls cell proliferation and modulates mitochondrial physiology in pancreatic stellate cells
Authors: Estaras Matias , Ortiz-Placin Candido , Castillejo-Rufo Alba , Fernandez-Bermejo Miguel , Blanco Gerardo , Mateos Jose M. , Vara Daniel , Gonzalez-Cordero Pedro L. , Chamizo Sandra , Lopez Diego , Rojas Adela , Jaen Isabel , de Armas Noelia , Salido Gines M. , Iovanna Juan L. , Santofimia-Castaño Patricia , Gonzalez Antonio 
Published: November 2022

AbstractWe have investigated the effects of melatonin on major pathways related with cellular proliferation and energetic metabolism in pancreatic stellate cells. In the presence of melatonin (1 mM, 100 µM, 10 µM, or 1 µM), decreases in the phosphorylation of c-Jun N-terminal kinase and of p44/42 and an increase in the phosphorylation of p38 were observed. Cell viability dropped in the presence of melatonin. A rise in the phosphorylation of AMP-activated protein kinase was detected in the presence of 1 mM and 100 µM melatonin. Treatment with 1 mM melatonin decreased the phosphorylation of protein kinase B, whereas 100 µM and 10 µM melatonin increased its phosphorylation. An increase in the generation of mitochondrial reactive oxygen species and a decrease of mitochondrial membrane potential were noted following melatonin treatment. Basal and maximal respiration, ATP production by oxidative phosphorylation, spare capacity, and proton leak dropped in the presence of melatonin. The expression of complex I of the mitochondrial respiratory chain was augmented in the presence of melatonin. Conversely, in the presence of 1 mM melatonin, decreases in the expression of mitofusins 1 and 2 were detected. The glycolysis and the glycolytic capacity were diminished in cells treated with 1 mM or 100 µM melatonin. Increases in the expression of phosphofructokinase-1 and lactate dehydrogenase were noted in cells incubated with 100 µM, 10 µM, or 1 µM melatonin. The expression of glucose transporter 1 was increased in cells incubated with 10 µM or 1 µM melatonin. Conversely, 1 mM melatonin decreased the expression of all three proteins. Our results suggest that melatonin, at pharmacological concentrations, might modulate mitochondrial physiology and energy metabolism in addition to major pathways involved in pancreatic stellate cell proliferation.
Detection of brook trout in spatiotemporally separate locations using validated eDNA technology
Authors: Nolan Kathleen P. , Loeza-Quintana Tzitziki , Little Hannah A. , McLeod Joschka , Ranger Benjamin , Borque Danielle A. , Hanner Robert H. 
Published: November 2022

AbstractBrook trout are a species of conservation concern in Southwestern Ontario, Canada, and effective monitoring of their populations is crucial for making informed management decisions. Electrofishing is a traditional, yet invasive, method that allows for fish abundance estimation. Environmental DNA (eDNA) is an emerging molecular tool that presents a non-invasive alternative to electrofishing. This study was a collaborative effort between researchers in academia, industry, and an NGO, with the following objectives: 1) compare eDNA detections with electrofishing when monitoring brook trout populations in a site of known occupancy, 2) compare existing eDNA collection methods, and 3) extend eDNA surveys to regions of unknown occupancy that could be of conservation concern (Hanlon Creek and Twelve Mile Creek, Ontario). First, eDNA sampling methods were validated with standard electrofishing. Water samples were filtered in tandem at each site using two commercially available eDNA samplers. The results suggest a significant difference in total eDNA capture and incidence of PCR inhibitors between the two autosamplers. Brook trout eDNA was detected at all locations in Hanlon Creek in September and November, as well as 5/6 sampling locations in Twelve Mile Creek. Brook trout signal in Hanlon Creek was stronger in November compared to September 2019, suggesting possible spawning activity. Brook trout eDNA was also detected in Twelve Mile Creek where brook trout were previously unreported. This study provides a technical validation for the use of eDNA in brook trout monitoring and illustrates the opportunity to use eDNA surveys in regulated settings to complement and improve conventional biomonitoring methods for the management of elusive species.
Characteristics and outcomes of acute hepatitis of unknown etiology in Egypt: first report of adult adenovirus-associated hepatitis
Authors: Ramadan Haidi Karam-Allah , Sayed Ibrahim M. , Elkhawaga Amal A. , Meghezel El-Zahraa M. , Askar Ashraf A. , Moussa Abdelmajeed M. , Osman Asmaa O. B. S. , Elfadl Azza Abo , Khalifa Walaa A. , Ashmawy Ahmed M. , El-Mokhtar Mohamed A. 
Published: November 2022

Abstract
Purpose
Several outbreaks of acute hepatitis of unknown etiology (AHUE) in children were reported in 2022 in many countries, with adenovirus identified as the etiological agent in most of them. We aimed to evaluate the characteristics and outcomes of AHUE cases in Egypt.

Methodology
Hospitalized patients with acute hepatitis were included in the study. Drug-induced, alcoholic hepatitis, autoimmune hepatitis, and Wilson’s disease were identified either by medical history or by routine laboratory diagnosis. Molecular and serological approaches were used to investigate common viral causes of hepatitis, such as hepatitis A–E viruses, cytomegalovirus, Epstein–Barr virus, herpes simplex viruses (HSV1/2), adenovirus, parvovirus B19, and coxsackie virus.

Results
A total of 42 patients were recruited and divided into two groups: 24 cases of unknown hepatitis after excluding the common causes and 18 cases of known hepatitis. About two-thirds of the patients were male (61.9%), and the mean age was 34.55 ± 16.27 years. Jaundice, dark urine, abdominal pain and diarrhea were recorded at a higher incidence in group 1, while jaundice and fever were frequent in group 2. Fulminant hepatitis occurred in 28.6% of the cases, but the two groups did not differ significantly in terms of patient outcome, duration of hospitalization, ascites, and development of fulminant hepatitis. Adenovirus was detected in five cases (20.8%) in group 1, and one case co-infecting with hepatitis E virus in group 2. Herpes simplex virus 1/2, coxsackie virus, and parvovirus B19 were not detected in any case, while etiologies of 75% of the cases were still not confirmed. One out of the six adenovirus-infected patients died. The outcome significantly correlated with the severity of the liver disease.

Conclusion
This is the first report describing etiologies and characteristics of AHUE cases in Egypt, and interestingly, adenovirus was detected in adults. Further studies are required to determine the prevalence of this newly emerging viral hepatitis pathogens.
Field-driven merging of polarizations and enhanced electrocaloric effect in BaTiO3-based lead-free ceramics
Authors: Niu Xiang , Jian Xiaodong , Gong Weiping , Liang Wei , Gong Xuetian , Zhang Guangzu , Jiang Shenglin , Yu Kun , Zhao Xiaobo , Yao Yingbang , Tao Tao , Liang Bo , Lu Sheng-Guo 
Published: November 2022

AbstractSolid-state cooling technology based on electrocaloric effect (ECE) has been advanced as an alternative to replace the vapour-compression approach to overcome the releasing of the global warming gases. However, the development in high ECE materials is still a challenge. In this work, polarization merging strategy was proposed to achieve a large ECE in xBa(Sn0.07Ti0.93)O3-(1−x)Ba(Hf0.1Ti0.9)O3 ferroelectric ceramics, where x = 0, 0.2, 0.4, 0.6, 0.8, and 1. Ba(Sn0.07Ti0.93)O3 with an orthorhombic phase and Ba(Hf0.1Ti0.9)O3 with a rhombohedral phase at room temperature were prepared beforehand as precursors, and phase-coexisted xBSnT-(1−x)BHfT ceramics were formed via a solid-state reaction approach. Phase coexisting structures were confirmed using the X-ray diffraction. The merged polarization was confirmed by the dielectric and ferroelectric properties. Optimal ECEs were obtained for 0.2BSnT-0.8BHfT ceramics, i.e., adiabatic temperature change ΔT = 2.16±0.08 K at 80 °C and 5 MV/m, and ΔT = 3.35±0.09 K at 80 °C and 7 MV/m.