bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electro-active metaobjective from metalenses-on-demand
Authors: Karst Julian , Lee Yohan , Floess Moritz , Ubl Monika , Ludwigs Sabine , Hentschel Mario , Giessen Harald 
Published: November 2022

AbstractSwitchable metasurfaces can actively control the functionality of integrated metadevices with high efficiency and on ultra-small length scales. Such metadevices include active lenses, dynamic diffractive optical elements, or switchable holograms. Especially, for applications in emerging technologies such as AR (augmented reality) and VR (virtual reality) devices, sophisticated metaoptics with unique functionalities are crucially important. In particular, metaoptics which can be switched electrically on or off will allow to change the routing, focusing, or functionality in general of miniaturized optical components on demand. Here, we demonstrate metalenses-on-demand made from metallic polymer plasmonic nanoantennas which are electrically switchable at CMOS (complementary metal-oxide-semiconductor) compatible voltages of ±1 V. The nanoantennas exhibit plasmonic resonances which can be reversibly switched ON and OFF via the applied voltage, utilizing the optical metal-to-insulator transition of the metallic polymer. Ultimately, we realize an electro-active non-volatile multi-functional metaobjective composed of two metalenses, whose unique optical states can be set on demand. Overall, our work opens up the possibility for a new level of electro-optical elements for ultra-compact photonic integration.
Compact zinc finger base editors that edit mitochondrial or nuclear DNA in vitro and in vivo
Authors: Willis Julian C. W. , Silva-Pinheiro Pedro , Widdup Lily , Minczuk Michal , Liu David R. 
Published: November 2022

AbstractDddA-derived cytosine base editors (DdCBEs) use programmable DNA-binding TALE repeat arrays, rather than CRISPR proteins, a split double-stranded DNA cytidine deaminase (DddA), and a uracil glycosylase inhibitor to mediate C•G-to-T•A editing in nuclear and organelle DNA. Here we report the development of zinc finger DdCBEs (ZF-DdCBEs) and the improvement of their editing performance through engineering their architectures, defining improved ZF scaffolds, and installing DddA activity-enhancing mutations. We engineer variants with improved DNA specificity by integrating four strategies to reduce off-target editing. We use optimized ZF-DdCBEs to install or correct disease-associated mutations in mitochondria and in the nucleus. Leveraging their small size, we use a single AAV9 to deliver into heart, liver, and skeletal muscle in post-natal mice ZF-DdCBEs that efficiently install disease-associated mutations. While off-target editing of ZF-DdCBEs is likely too high for therapeutic applications, these findings demonstrate a compact, all-protein base editing research tool for precise editing of organelle or nuclear DNA without double-strand DNA breaks.
Engineered disorder in CO2 photocatalysis
Authors: Li Zhao , Mao Chengliang , Pei Qijun , Duchesne Paul N. , He Teng , Xia Meikun , Wang Jintao , Wang Lu , Song Rui , Ali Feysal M. , Meira Débora Motta , Ge Qingjie , Ghuman Kulbir Kaur , He Le , Zhang Xiaohong , Ozin Geoffrey A. 
Published: November 2022

AbstractLight harvesting, separation of charge carriers, and surface reactions are three fundamental steps that are essential for an efficient photocatalyst. Here we show that these steps in the TiO2 can be boosted simultaneously by disorder engineering. A solid-state reduction reaction between sodium and TiO2 forms a core-shell c-TiO2@a-TiO2-x(OH)y heterostructure, comprised of HO-Ti-[O]-Ti surface frustrated Lewis pairs (SFLPs) embedded in an amorphous shell surrounding a crystalline core, which enables a new genre of chemical reactivity. Specifically, these SFLPs heterolytically dissociate dihydrogen at room temperature to form charge-balancing protonated hydroxyl groups and hydrides at unsaturated titanium surface sites, which display high reactivity towards CO2 reduction. This crystalline-amorphous heterostructure also boosts light absorption, charge carrier separation and transfer to SFLPs, while prolonged carrier lifetimes and photothermal heat generation further enhance reactivity. The collective results of this study motivate a general approach for catalytically generating sustainable chemicals and fuels through engineered disorder in heterogeneous CO2 photocatalysts.
Smc5/6’s multifaceted DNA binding capacities stabilize branched DNA structures
Authors: Chang Jeremy T-H. , Li Shibai , Beckwitt Emily C. , Than Thane , Haluska Cory , Chandanani Joshua , O’Donnell Michael E. , Zhao Xiaolan , Liu Shixin 
Published: November 2022

AbstractSmc5/6 is an evolutionarily conserved SMC complex with roles in DNA replication and repair, as well as in viral DNA restriction. Understanding its multiple functions has been hampered by a lack of mechanistic studies on how the Smc5/6 complex associates with different types of DNA. Here we address this question by simultaneously visualizing the behavior of Smc5/6 on three types of DNA, namely double-stranded (ds) DNA, single-stranded (ss) DNA, and junction DNA formed by juxtaposed ss- and dsDNA, using correlative single-molecule fluorescence and force microscopy. We find that Smc5/6 displays distinct behaviors toward different types of DNA, dynamically associating with dsDNA while stably binding to junction DNA. Mechanistically, both the Nse1-3-4 subcomplex and ATP binding enhance the complex’s dsDNA association. In contrast, Smc5/6’s assembly onto ssDNA emanating from junction DNA, which occurs even in the presence high-affinity ssDNA binders, is aided by Nse1-3-4, but not by ATP. Moreover, we show that Smc5/6 protects junction DNA stability by preventing ssDNA annealing. The multifaceted DNA association behaviors of Smc5/6 provide a framework for understanding its diverse functions in genome maintenance and viral DNA restriction.
Inflammatory bone marrow signaling in pediatric acute myeloid leukemia distinguishes patients with poor outcomes
Authors: Bolouri Hamid , Ries Rhonda E. , Wiedeman Alice E. , Hylkema Tiffany , Scheiding Sheila , Gersuk Vivian H. , O’Brien Kimberly , Nguyen Quynh-Anh , Smith Jenny L. , Alice Long S. , Meshinchi Soheil 
Published: November 2022

AbstractHigh levels of the inflammatory cytokine IL-6 in the bone marrow are associated with poor outcomes in pediatric acute myeloid leukemia (pAML), but its etiology remains unknown. Using RNA-seq data from pre-treatment bone marrows of 1489 children with pAML, we show that > 20% of patients have concurrent IL-6, IL-1, IFNα/β, and TNFα signaling activity and poorer outcomes. Targeted sequencing of pre-treatment bone marrow samples from affected patients (n = 181) revealed 5 highly recurrent patterns of somatic mutation. Using differential expression analyses of the most common genomic subtypes (~60% of total), we identify high expression of multiple potential drivers of inflammation-related treatment resistance. Regardless of genomic subtype, we show that JAK1/2 inhibition reduces receptor-mediated inflammatory signaling by leukemic cells in-vitro. The large number of high-risk pAML genomic subtypes presents an obstacle to the development of mutation-specific therapies. Our findings suggest that therapies targeting inflammatory signaling may be effective across multiple genomic subtypes of pAML.
Phase separation modulates the assembly and dynamics of a polarity-related scaffold-signaling hub
Authors: Tan Wei , Cheng Sihua , Li Yingying , Li Xiao-Yang , Lu Ning , Sun Jingxian , Tang Guiyue , Yang Yujiao , Cai Kezhu , Li Xuefei , Ou Xijun , Gao Xiang , Zhao Guo-Ping , Childers W. Seth , Zhao Wei 
Published: November 2022

AbstractAsymmetric cell division (ACD) produces morphologically and behaviorally distinct cells and is the primary way to generate cell diversity. In the model bacteriumCaulobacter crescentus, the polarization of distinct scaffold-signaling hubs at the swarmer and stalked cell poles constitutes the basis of ACD. However, mechanisms involved in the formation of these hubs remain elusive. Here, we show that a swarmer-cell-pole scaffold, PodJ, forms biomolecular condensates both in vitro and in living cells via phase separation. The coiled-coil 4–6 and the intrinsically disordered regions are the primary domains that contribute to biomolecular condensate generation and signaling protein recruitment in PodJ. Moreover, a negative regulation of PodJ phase separation by the stalked-cell-pole scaffold protein SpmX is revealed. SpmX impedes PodJ cell-pole accumulation and affects its recruitment ability. Together, by modulating the assembly and dynamics of scaffold-signaling hubs, phase separation may serve as a general biophysical mechanism that underlies the regulation of ACD in bacteria and other organisms.
Welche Kommunikationstechniken sind bei Physiotherapeut*innen während einer Bewegungsberatung mit Personen mit axialer Spondyloarthritis identifizierbar? – Eine Querschnittstudie
Authors: Vogt Ulisse Patrizio , Rausch Anne-Kathrin , Niedermann Karin 
Published: November 2022

Zusammenfassung
Hintergrund Die Schweizerische Vereinigung Morbus Bechterew (SVMB) hat kürzlich das BeFit-Konzept in der Gruppentherapie für Personen mit axialer Spondyloarthritis (axSpA, umgangssprachlich M. Bechterew) implementiert. Ein wichtiger Bestandteil des BeFit-Konzepts ist eine gezielte Bewegungsberatung zur Förderung von selbstständigem Training. Die Kommunikationskompetenz von Physiotherapeut*innen (PT) gilt dabei als Voraussetzung für eine gelungene und wirksame Patientenedukation (PE), ist bisher aber wenig untersucht.
Ziel Analyse, welche Kommunikationstechniken PT während der Bewegungsberatung mit Personen mit axSpA anwenden.
Methode Es wurde eine Querschnittstudie mit PT und Personen mit axSpA der SVMB-Gruppentherapien durchgeführt. Die Bewegungsberatungen wurden mit Audio aufgenommen, die Kommunikationstechniken anhand des Manuals „The Calgary Cambridge Referenced Observation Guide One“ identifiziert. Das Manual enthält 5 Gesprächsabschnitte (1. Einleiten der Sitzung, 2. Informationssammlung, 3. Strukturierung der Konsultation, 4. Beziehungsaufbau, 5. Beenden der Sitzung) mit 38 Techniken, wovon 6 nicht analysiert werden konnten. Eingeschlossen wurden die Techniken, die mindestens einmal identifiziert wurden. Je strukturierter die Beratung war und je mehr Techniken identifiziert wurden, desto besser konnte die Kommunikation der PT während der Beratungsgespräche bewertet werden.
Ergebnisse Es nahmen 12 PT und 41 Personen mit axSpA teil. Somit wurden 41 Bewegungsberatungen ausgewertet. Insgesamt wurden bei 10 von 12 (85 %) der PT in allen Beratungen die 32 eingeschlossenen Kommunikationstechniken mit einer Häufigkeit der einzelnen Techniken zwischen einmal und 41-mal identifiziert. Der Gesprächsabschnitt „Strukturierung der Konsultation“ zeigte die höchste Häufigkeit zwischen 29- und 41-mal.
Schlussfolgerung Die strukturierte Kommunikation ist ein wesentlicher Bestandteil der PE. Die Ergebnisse zeigen, dass die Mehrheit der PT die Bewegungsberatungen mit Patient*innen mit axSpA strukturiert durchführten und die Kommunikationstechniken umfassend identifiziert werden konnten. Die unterschiedlich häufige Anwendung der einzelnen Techniken kann dazu genutzt werden, die Kommunikation der PT in den Bewegungsberatungen für Personen mit axSpA gezielt zu schulen und zu optimieren.
The concept of register in heritage language retention
Authors: Olfert Helena 
Published: November 2022

AbstractThis paper argues for a greater consideration of the role of register in the retention of heritage languages by analyzing data on adolescent heritage-language speakers in Germany. The study addresses the following question: Which language external factors have the greatest impact on heritage-language retention in the intimate vs formal registers? A questionnaire was filled out by 202 students (age 15–17) in an urban area in Germany, who are all heritage-language speakers of 42 different languages. The major finding of this study is that heritage-language retention is determined by different language-external factors in each register. The fact that different models of heritage-language retention can be postulated for different language registers emphasizes the need to describe the language competences of heritage-language speakers according to registers.
Social interaction in local public squares after dark
Authors: Hennig VKR , Fotios S , Gentile N , Sternudd C , Johansson M 
Published: November 2022

Abstract
This paper explores social interaction in local public squares under different lighting conditions. At its best public squares are social spaces that engender a sense of belonging, increase the quality of life and wellbeing of individuals. It is proposed that outdoor lighting would be essential to the use of the public realm after dark, but empirical results regarding lighting conditions and social aspects of life in public squares are limited. Based on a socio-physical conceptual model of the transactional relationship of the user, the lit environment and the behavioural outcome, this study investigated active social interaction in daylight compared to after dark. A field study was conducted in two local public squares in Malmö, Sweden. The occurrences of which visitors were being alone, in pairs, or in groups of three or more (N=2522), and verbal or non-verbal interaction amongst those in company of another person were recorded. The lit appearance of the two squares after dark, was assessed with HDR-photography and photometric measurements; portraying dissimilar spatial, spectral and intensity characteristics. The results of social interaction show dissimilar patterns of the two squares; an increase in social interaction in EL after dark was observed in one of the squares, while a decrease in the afternoon and no significant difference was displayed in the evening after dark in the other square. It is suggested that lighting may sustain patterns of social interaction after dark, however it might be, that the company of another is especially important after dark.
True specs for dimmed and colour tuned LED luminaires
Authors: Dam-Hansen C , Thorseth A , Corell D D , Bay A , Krause C , Kofod C 
Published: November 2022

Abstract
Colour tunable and dimmable LED lamps and LED luminaires are used increasingly in both domestic and professional lighting sector. The documentation of energy and light quality performance are often very limited for only for a single setting of multiple possible settings. Fifteen LED lamps and eight LED luminaires that are dimmable and tuneable white have been selected for demonstration of the variations in these parameters and possible errors in lighting design simulations. These have been tested in over twenty modes of operation including dimming from 100% to 25%, and correlated colour temperature (CCT) settings from 2200K to 6500K. The inherent standby power of these colour tuneable devices is measured, and the overall efficacy is evaluated in normal usage situations. In average for all tested LED lamps and luminaires 49% and 33% of the energy consumption is used in standby mode for 1h and 2h on time pr. day, respectively. The extensive set of test results show that there can be very large variations in e.g. the luminous flux, luminous efficacy and stroboscopic visibility measure (SVM), while others are very consistent over the modes of operation. The luminous flux shows variations of down to 22% of the maximum and rated value, and these lowest values occurs at the lowest CCT. The best performing has over 90% at all CCT settings. For the luminous efficacy most test results show a maximum value at 4000K or at the maximum CCT, and variations down to 20-50% of the maximum efficacy. The SVM is shown to increase to values over 1 and up to 2.4 at low dimming levels or low light output, demonstrating that stroboscopic effect will be visible for the majority of people and hence a problem in such settings. This is seen for approx. half the tested while the others have SVM < 0.4 for all settings and will not be visible over all settings. These results demonstrate the need for testing over the possible modes of operation to be able to correctly design and simulate a lighting installation using dimmable and colour tunable LED lamps and luminaires. The needed new dataformats and adoption of these from test laboratories to lighting software, in order for lighting designers and energy engineers to be able to make use of the extensive test results, are discussed.
Window geometry and its effect on the experience of illuminated spaces – a study of three daylit architectural cases
Authors: Mathiasen N , Grønlund L , Frandsen A K , Harild M 
Published: November 2022

Abstract
Looking at the architecture of modern housing in Denmark, it seems to express an understanding that equals more daylight with good daylight. With coated energy glass, the windows have increased in size, making glass into a façade material. This means that windows are no longer just holes in the façade but rather make up the entire façade itself. This changes the spatial relationship between the window design and distribution of daylight within the interior space, though we seldom address this lacking a vocabulary and methods. This paper sets out to investigate and experiment with how we can describe and document this change in daylight conditions and how these influence our visual environment, using photographs to record this. The methodology focusses on observations of daylit spaces in three different housing examples in Copenhagen from the 1800s to today. By observing the three daylit spaces, it becomes clear that differences in the size, shape, and position of window apertures influence the qualities of light in a space significantly. Considering that when it comes to window apertures different designs affect the experience of light in a space significantly, it is important to take this into account when designing with daylight. It is also important from a sustainability point of view to include this awareness in future design approaches.
Energy-efficient lighting and visually impaired users in homelike environments
Authors: Mattsson P 
Published: November 2022

Abstract
Despite a rapid transition to LED lamps, it is still little knowledge about the effects of these light sources on users with low vision. The study’s main objective was to acquire a better understanding about how correlated colour temperatures (CCTs) of non-directional LED affect visual accessibility and perceptions of lighting quality in homelike environments among visually impaired persons. The study took place in a full scale laboratory in which CCTs of 2,700 K and 4,000 K were tested. Participants having normal vision (N=60, 24 males, 36 females, M = 25.50) were divided into three groups of 20 to assess the two lit environments with cataract goggle, severe cataract goggle and normal vision respectively. They were asked to perform everyday activities while the observer recorded the time and conducted observations, and to rate the difficulty of each activity. Thereafter, the participants were asked to rate their perceptions of lighting quality and contrasts in the room. With the same CRI of Ra80, there were no differences in the performance of everyday activities between the two CCTs whereas the differences in the perceived lighting quality and the perceived contrasts were found. The cool white CCT was found to positively affect visual accessibility and perceived contrasts however, its comfort quality remains questionable.
Rigidity Theorems by Capacities and Kernels
Authors: Dong Robert Xin , Treuer John N , Zhang Yuan 
Published: November 2022

Abstract
For any open hyperbolic Riemann surface $X$, the Bergman kernel $K$, the logarithmic capacity $c_{\beta }$, and the analytic capacity $c_{B}$ satisfy the inequality chain $\pi K \geq c^2_{\beta } \geq c^2_B$. Moreover, equality holds at a single point between any two of the three quantities if and only if $X$ is biholomorphic to a disk possibly less a relatively closed polar set. We extend the inequality chain by showing that $c_{B}^2 \geq \pi v^{-1}(X)$ on planar domains, where $v(\cdot )$ is the Euclidean volume, and characterize the extremal cases when equality holds at one point. Similar rigidity theorems concerning the Szegö kernel, the higher-order Bergman kernels, and the sublevel sets of the Green’s function are also developed. Additionally, we explore rigidity phenomena related to the multi-dimensional Suita conjecture.
Current Issues in Blood Gas Analysis
Authors: Korpi-Steiner Nichole , Horowitz Gary , Tesfazghi Merih , Suh-Lailam Brenda B 
Published: November 2022

Abstract

Background
Blood gas analysis constitutes one of the most widely used tests, especially in critical care settings such as intensive care units, emergency departments, and operating rooms. Blood gas results are key for assessing acid–base balance and ventilatory control in critically ill patients. Because blood gas analysis plays a vital role in management of critically ill patients, this testing is frequently conducted at the point-of-care by users with various educational backgrounds across different hospital departments.

Content
When performing blood gas analysis, it is important to be aware of the analytical issues that may affect the different components of this testing. With blood gas analysis, differences in test names and method changes over time have led to several controversies that might affect test result interpretations. Hence, being aware of these controversies is important in ensuring appropriateness of result interpretations. Many blood gas testing programs face challenges with maintaining quality assurance. Having practical approaches to method verification, and choosing the right blood gas analyzer type, will go a long way to ensure quality in blood gas analysis.

Summary
We review analytical issues and controversies associated with blood gas testing, as well as practical approaches to deciding on a blood gas analyzer and quality assurance.
CT contrast agents and injection protocols
Authors: Mihl Casper , Martens Bibi 
Published: November 2022

Abstract
Diagnostic image quality in coronary CT angiography is influenced by scan and contrast media (CM) parameters, as well as patient-related factors. Individualized CM injection protocols are aimed at optimizing scan and injection parameters tailored to the patient with the ultimate goal of achieving diagnostic image quality in all patients. This chapter aims to outline the influence of various essential CM parameters. In addition, theoretical and practical information is provided about general adverse reactions, treatment and other informative topics related to CM.
CT-based fractional flow reserve
Authors: Mastrodicasa Domenico 
Published: November 2022

Abstract
CT-derived fractional flow reserve (CT-FFR) is an advanced postprocessing technique that uses coronary CT angiography (CCTA) to compute non-invasively the functional significance of a coronary stenosis and inform treatment decisions. CT-FFR applies the principles of computational fluid dynamics to the patient-specific anatomical three-dimensional model of the coronary tree obtained with the CCTA. As a result, CT-FFR can be computed and displayed at any point along the coronary vessels. This chapter includes practical information to help the physician using CT-FFR in routine clinical practice, from the indications to interpretation tips and reporting approach.