bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermodynamic and kinematic structure of tropical cyclones in the western North Pacific based on ACARS/AMDAR
Authors: Ito Kosuke , Yamamoto Ren 
Published: December 2022

Meteorological variables are often reported by commercial aircraft flying around tropical cyclones (TCs). They are archived in Aircraft Communications, Addressing, and Reporting System/Aircraft Meteorological Data Relay (ACARS/AMDAR). Therefore, they are potentially useful for constructing a composite mean structure of TCs based on in-situ measurements. The number of temperature and wind observations are 4.0 × 106 and 1.0 × 104 within the radius of 1,200 km and 100 km from the TC center during 2010–2020, respectively. The warm-core potential temperature anomaly with respect to the climatology is 6.4 K, 9.1 K, and 14.4 K maximized around 300 hPa for weak, moderate, and strong TCs, respectively. The composite of the potential temperature anomaly potentially extends more than 1,000 km from the TC center in the upper troposphere, cautioning the typical definition of the environment. The region of significant upper-level positive potential temperature anomalies extends broadly with increasing TC intensity. Moreover, large TCs tend to have a broad and deep upper-level warm core for a given intensity. In addition, we ensured that a single observation of potential temperature around 300 hPa could be used as a proxy for minimum sea level pressure. Low-level inflow and upper-level outflow were detected in the ACARS/AMDAR data.
The impact of hyperglycaemic crisis episodes on long-term outcomes for inpatients presenting with acute organ injury: A prospective, multicentre follow-up study
Authors: Duan Zixiao , Song Peiyang , Yang Cheng , Deng Liling , Jiang Youzhao , Deng Fang , Jiang Xiaoyan , Chen Yan , Yang Gangyi , Ma Yu , Deng Wuquan 
Published: December 2022

BackgroundThe long-term clinical outcome of poor prognosis in patients with diabetic hyperglycaemic crisis episodes (HCE) remains unknown, which may be related to acute organ injury (AOI) and its continuous damage after hospital discharge. This study aimed to observe the clinical differences and relevant risk factors in HCE with or without AOI.MethodsA total of 339 inpatients were divided into an AOI group (n=69) and a non-AOI group (n=270), and their differences and risk factors were explored. The differences in clinical outcomes and prediction models for evaluating the long-term adverse events after hospital discharge were established.ResultsThe mortality among cases complicated by AOI was significantly higher than that among patients without AOI [8 (11.59%) vs. 11 (4.07%), Q = 0.034] during hospitalization. After a 2-year follow-up, the mortality was also significantly higher in patients with concomitant AOI than in patients without AOI after hospital discharge during follow-up [13 (21.31%) vs. 15 (5.8%), Q &lt; 0.001]. The long-term adverse events in patients with concomitant AOI were significantly higher than those in patients without AOI during follow-up [15 (24.59%) vs. 31 (11.97%), Q = 0.015]. Furthermore, Blood β-hydroxybutyric acid (P = 0.003), Cystatin C (P &lt;0.001), serum potassium levels (P = 0.001) were significantly associated with long-term adverse events after hospital discharge.ConclusionsThe long-term prognosis of HCE patients complicated with AOI was significantly worse than that of HCE patients without AOI. The laboratory indicators were closely correlated with AOI, and future studies should explore the improvement of clinical outcome in response to timely interventions.
Citrullinated glucose-regulated protein 78 is a candidate target for melanoma immunotherapy
Authors: Brentville Victoria Anne , Symonds Peter , Chua JiaXin , Skinner Anne , Daniels Ian , Cook Katherine Wendy , Koncarevic Sasa , Martinez-Pinna Roxana , Shah Sabaria , Choudhury Ruhul Hasan , Vaghela Poonam , Weston Daisy , Al-Omari Abdullah , Davis James , Durrant Lindy G. 
Published: December 2022

IntroductionPost translational modification of proteins plays a significant role in immune recognition. In particular the modification of arginine to citrulline which is mediated by PAD enzymes is increased during cellular stress (autophagy) which permits the presentation of modified epitopes upon MHC class II molecules for recognition by CD4 T cells. Citrullination also occurs in tumour cells as a result of continuous environmental stresses and increased autophagy. We have shown in animal models the efficient stimulation of citrullinated epitope specific CD4 T cells resulting in dramatic elimination/regression of tumours. The ER chaperone glucose-regulated protein 78 (GRP78) is known to also be required for stress-induced autophagy and is directly linked to autophagosome formation. GRP78 is known to be highly expressed by many tumour types. In this study we investigate the potential of targeting citrullinated GRP78 for cancer therapy.MethodsA citrullinated GRP78 specific antibody was used to assess citrullinated GRP78 expression in murine and human tumour cells by flow cytometry. Five peptides were selected and used to vaccinate HLA transgenic mice and immune responses were characterised by ex vivo cytokine ELISpot assay. T cell repertoire in humans was assessed through proliferation assays and cytokine ELISpot assay. Citrullinated peptide was identified in murine B16 melanoma by mass spectrometry and the peptide vaccine was assessed for tumour therapy in a mouse melanoma model.ResultsWe show the identification CD4 T cell responses to one citrullinated GRP78 epitope that are restricted through HLA DP*0401 and HLA-DR*0101 alleles. This peptide is detected by mass spectrometry in B16 melanoma grown in vivo and citrulline specific CD4 responses to two peptides spanning this epitope mediate efficient therapy of established B16 melanoma tumours in HHDII/DP4 (p&lt;0.0001) transgenic mouse model. Finally, we demonstrate the existence of a repertoire of responses to the citrullinated GRP78 peptide in healthy individuals (p=0.0023) with 13/17 (76%) individuals showing a response to this peptide.ConclusionWe propose that citrullinated GRP78 is a candidate tumour antigen and vaccination against citrullinated GRP78 may provide a promising tumour therapy approach.
Altered gut fungi in systemic lupus erythematosus – A pilot study
Authors: Li Bao-Zhu , Wang Hua , Li Xian-Bao , Zhang Qian-Ru , Huang Rong-Gui , Wu Hong , Wang Yi-Yu , Li Kai-Di , Chu Xiu-Jie , Cao Nv-Wei , Zhou Hao-Yue , Fang Xin-Yu , Leng Rui-Xue , Fan Yin-Guang , Tao Jin-Hui , Shuai Zong-Wen , Ye Dong-Qing 
Published: December 2022

ObjectiveGut fungi, as symbiosis with the human gastrointestinal tract, may regulate physiology via multiple interactions with host cells. The plausible role of fungi in systemic lupus erythematosus (SLE) is far from clear and need to be explored.MethodsA total of 64 subjects were recruited, including SLE, rheumatoid arthritis (RA), undifferentiated connective tissue diseases (UCTDs) patients and healthy controls (HCs). Fecal samples of subjects were collected. Gut fungi and bacteria were detected by ITS sequencing and 16S rRNA gene sequencing, respectively. Alpha and beta diversities of microbiota were analyzed. Linear discriminant analysis effect size analysis was performed to identify abundance of microbiota in different groups. The correlation network between bacterial and fungal microbiota was analyzed based on Spearman correlation.ResultsGut fungal diversity and community composition exhibited significant shifts in SLE compared with UCTDs, RA and HCs. Compared with HCs, the alpha and beta diversities of fungal microbiota decreased in SLE patients. According to principal coordinates analysis results, the constitution of fungal microbiota from SLE, RA, UCTDs patients and HCs exhibited distinct differences with a clear separation between fungal microbiota. There was dysbiosis in the compositions of fungal and bacterial microbiota in the SLE patients, compared to HCs. Pezizales, Cantharellales and Pseudaleuria were enriched in SLE compared with HCs, RA and UCTDs. There was a complex relationship network between bacterial and fungal microbiota, especially Candida which was related to a variety of bacteria.ConclusionThis study presents a pilot analysis of fungal microbiota with diversity and composition in SLE, and identifies several gut fungi with different abundance patterns taxa among SLE, RA, UCTDs and HCs. Furthermore, the gut bacterial-fungal association network in SLE patients was altered compared with HCs.
Transcutaneous electrical acupoint stimulation for reducing cognitive dysfunction in lumbar spine surgery: A randomized, controlled trail
Authors: Wang Li-feng , Liang Wei-dong , Wang Bing-yu , Guo Ming-ling , Zhou Jian-shun , Chen Li , Zhong Mao-lin , Ye Jun-ming 
Published: December 2022

ObjectiveThis study aimed to evaluate the effect of perioperative transcutaneous electrical acupoint stimulation (TEAS) on postoperative cognitive dysfunction (POCD) in older patients with lumbar spine surgery.MethodsOlder patients (aged 60–80 years old) receiving lumbar spine surgery under general anesthesia were randomly divided into group A, 3-day intervention group; group B, 7-day intervention group; control group C, sham TEAS group, selected “Baihui” (GV 20) and “Dazhui” (GV 14) point was intervened once 30 min before operation with “HANS” transcutaneous electrical stimulation device, and then once a day after operation for 30 min each time. The primary outcome was the incidence of postoperative cognitive impairment assessed by the use of the Mini Mental Rating Scale (MMSE), patients developed POCD according to the Z score method. The secondary outcome was serum interleukin-6 (IL-6), tumor Necrosis factor α (TNF-α), neuron-specific enolase (NSE), and S100β protein levels.ResultsThree days after surgery, the incidence of POCD in groups A((22.4%)) and B ((18.3%)) were lower than those in group C ((42.9%)) (P &lt; 0.05). There was no significant difference between groups A and B (P &gt; 0.05). Seven days after surgery, the incidence of POCD in group B (18.3%) was lower than that in groups A (26.5%) and B (42.9%), and the comparison between groups B and C was statistically significant (P &lt; 0.05). On the 3rd and 7th days after surgery, the levels of IL-6, TNF-α, NSE, and S100β in the two TEAS groups were lower than those in the sham TEAS group (P &lt; 0.01), but higher than the preoperative levels in the three groups (P &lt; 0.01).ConclusionIt seems that Perioperative TEAS intervention could reduce the level of inflammatory factors IL-6, TNF-α in the blood of older patients with lumbar spine surgery, and reduce the incidence of POCD.Clinical trial registrationwww.chictr.org.cn, identifier ChiCTR2200063030.
Maternal blood inflammatory marker levels increased in fetuses with ventriculomegaly
Authors: Li Qiang , Ju Xin-Wei , Xu Jing , Jiang Jiuhong , Lu Chang , Ju Xing-Da 
Published: December 2022

BackgroundFetal ventriculomegaly (VM) is one of the most common abnormalities of the central nervous system (CNS), which can be significantly identified by brain anomalies prenatally by magnetic resonance imaging (MRI). Aberrant white blood cells (WBCs) levels indicate that the maternal is suffering from the infection. Previous studies have confirmed that prenatal infection can affect fetal brain structure, but there is no research revealed the association between maternal blood parameters with fetal VM until now.MethodsWe measured the width of the lateral ventricle of 142 fetuses, which were divided into the fetal VM group (n = 70) and the normal lateral ventricle group (n = 72). We compared maternal blood cell levels between the two groups and investigate potential biomarkers of fetal VM.ResultHigh levels of maternal WBC and neutrophil (NE#) levels were observed in fetuses with VM (p &lt; 0.001), while lymphocyte percentage, monocytes (MO#), neutrophil/lymphocyte ratio (NLR), and platelet were also increased in the fetal VM group (p = 0.033, 0.027, 0.034, and 0.025, respectively). receiver–operator curve (ROC) analysis suggested that WBC and NE# counts might be useful to distinguish fetuses with enlarged lateral ventricles (AUC = 0.688, 0.678, respectively).ConclusionThe current study emphasizes the importance of maternal infection for fetal brain growth, which could provide important information for prenatal diagnosis of CNS anomalies. Future research needs longitudinal analysis and exploration of the influence of maternal blood inflammatory marker levels on fetal brain development.
CT-based deep learning radiomics signature for the preoperative prediction of the muscle-invasive status of bladder cancer
Authors: Chen Weitian , Gong Mancheng , Zhou Dongsheng , Zhang Lijie , Kong Jie , Jiang Feng , Feng Shengxing , Yuan Runqiang 
Published: December 2022

ObjectivesAlthough the preoperative assessment of whether a bladder cancer (BCa) indicates muscular invasion is crucial for adequate treatment, there currently exist some challenges involved in preoperative diagnosis of BCa with muscular invasion. The aim of this study was to construct deep learning radiomic signature (DLRS) for preoperative predicting the muscle invasion status of BCa.MethodsA retrospective review covering 173 patients revealed 43 with pathologically proven muscle-invasive bladder cancer (MIBC) and 130 with non–muscle–invasive bladder cancer (non- MIBC). A total of 129 patients were randomly assigned to the training cohort and 44 to the test cohort. The Pearson correlation coefficient combined with the least absolute shrinkage and selection operator (LASSO) was utilized to reduce radiomic redundancy. To decrease the dimension of deep learning features, Principal Component Analysis (PCA) was adopted. Six machine learning classifiers were finally constructed based on deep learning radiomics features, which were adopted to predict the muscle invasion status of bladder cancer. The area under the curve (AUC), accuracy, sensitivity and specificity were used to evaluate the performance of the model.ResultsAccording to the comparison, DLRS-based models performed the best in predicting muscle violation status, with MLP (Train AUC: 0.973260 (95% CI 0.9488-0.9978) and Test AUC: 0.884298 (95% CI 0.7831-0.9855)) outperforming the other models. In the test cohort, the sensitivity, specificity and accuracy of the MLP model were 0.91 (95% CI 0.551-0.873), 0.78 (95% CI 0.594-0.863) and 0.58 (95% CI 0.729-0.827), respectively. DCA indicated that the MLP model showed better clinical utility than Radiomics-only model, which was demonstrated by the decision curve analysis.ConclusionsA deep radiomics model constructed with CT images can accurately predict the muscle invasion status of bladder cancer.
Risk of paediatric multisystem inflammatory syndrome (PIMS-TS) during the SARS-CoV-2 alpha and delta variant waves: National observational and modelling study, 2020–21, England
Authors: Shingleton Joseph , Burton Lucy , Williams Hannah E. , Finnie Thomas J. R. , Bennett Emma , Birrell Paul , Kenny Simon , Watson-Koszel Tiffany , Viner Russell , Arditi Moshe , DeAngelis Daniela , Gent Nick , Ladhani Shamez N. 
Published: December 2022

ObjectivesPaediatric Multisystem Inflammatory Syndrome (PIMS-TS) is a rare life-threatening complication that typically occurs several weeks after SARS-CoV-2 infection in children and young people (CYP). We used national and regional-level data from the COVID-19 pandemic waves in England to develop a model to predict PIMS-TS cases.MethodsSARS-CoV-2 infections in CYP aged 0–15 years in England were estimated using the PHE-Cambridge real-time model. PIMS-TS cases were identified through the British Paediatric Surveillance Unit during (March-June 2020) and through Secondary Uses Services (SUS) from November 2020. A predictive model was developed to estimate PIMS-TS risk and lag times after SARS-CoV-2 infections.ResultsDuring the Alpha wave, the model accurately predicted PIMS-TS cases (506 vs. 502 observed cases), with a median estimated risk of 0.038% (IQR, 0.037–0.041%) of paediatric SARS-CoV-2 infections. For the Delta wave, the median risk of PIMS-TS was significantly lower at 0.026% (IQR, 0.025–0.029%), with 212 observed PIMS-TS cases compared to 450 predicted by the model.ConclusionsThe model accurately predicted national and regional PIMS-TS cases in CYP during the Alpha wave. PIMS-TS cases were 53% lower than predicted during the Delta wave. Further studies are needed to understand the mechanisms of the observed lower risk with the Delta variant.
The mechanism of Renshen-Fuzi herb pair for treating heart failure—Integrating a cardiovascular pharmacological assessment with serum metabolomics
Authors: Chen Xiaofei , Chen Yulong , Xie Shiyang , Wang Xiaoyan , Wu Yali , Zhang Hui , Zhao Ya , Jia Jinhao , Wang Bin , Li Weixia , Tang Jinfa , Xiao Xiaohe 
Published: December 2022

Background: Renshen-Fuzi herb pair (RS-FZ) is often used in the clinical treatment of heart failure (HF) and has a remarkable therapeutic effect. However, the mechanism of RS-FZ for treating HF remains unclear. In our study, we explored the mechanism of RS-FZ for treating HF.Methods: Evaluation of RS-FZ efficacy by cardiovascular pharmacology. Moreover, Global metabolomics profiling of the serum was detected by UPLC-QTOF/MS. Multivariate statistics analyzed the specific serum metabolites and corresponding metabolic pathways. Combining serum metabolomics with network pharmacology, animal experiments screened and validated the critical targets of RS-FZ intervention in HF.Results: RS-FZ significantly ameliorated myocardial fibrosis, enhanced cardiac function, and reduced the serum HF marker (brain natriuretic peptide) level in rats with HF. Through topological analysis of the “Metabolite-Target-Component” interaction network, we found that 79 compounds of RS-FZ directly regulated the downstream specific serum metabolites by acting on four critical target proteins (CYP2D6, EPHX2, MAOB, and ENPP2). The immunohistochemistry results showed that RS-FZ observably improved the expression of CYP2D6 and ENPP2 proteins while decreasing the expression of EPHX2 and MAOB proteins dramatically.Conclusion: The integrated cardiovascular pharmacological assessment with serum metabolomics revealed that RS-FZ plays a crucial role in the treatment of HF by intervening in CYP2D6, EPHX2, MAOB, and ENPP2 target proteins. It provides a theoretical basis for RS-FZ for treating HF.
Generation of phase-only holograms with high-diffraction-order reconstruction by a U-Net-based neural network: A phase grating perspective
Authors: Liu Xinlei , Yan Xingpeng , Wang Xi , Jing Tao , Li Pei , Song Cheng , Qu Qiang , Jiang Xiaoyu 
Published: December 2022

Implicit periodic structure in phase-only holograms will result in many diffraction orders in the diffraction field. We analyzed the diffraction pattern from a phase gratings point of view and proved that the diffraction orders were jointly influenced by the phase factor, the single-beam diffraction factor, and the multibeam interference factor. According to the analysis, we proposed the high-diffraction-order angular spectrum method (HDO-ASM) for the numerical reconstruction of high diffraction orders. Different from the conventional methods of removing high diffraction orders, we chose to reconstruct target images in high diffraction orders with HDO-ASM and a U-Net-based neural network. Finally, the 4 K phase-only holograms with high-diffraction-order reconstruction were generated in 0.09s and had a mean reconstruction quality of 34.3 dB (PSNR) in the DIV2K valid dataset. Theoretical and experimental results demonstrated that there are few speckle noises and fringes in the reconstructed images of holograms generated by the proposed method.
Effects of compression running pants and treadmill running stages on knee proprioception and fatigue-related physiological responses in half-marathon runners
Authors: Chang Lin , Fu Silin , Li Jianghua , Wu Sam , Adams Roger , Han Jia , Han Chunying 
Published: December 2022

Background: Knee injury is common in half-marathon runners, however, the effect of compression running pants on fatigue and knee proprioception remains unclear.Objectives: The study aims to investigate whether wearing compression running pants (CRP) and treadmill running stages affect knee proprioception and fatigue-related physiological responses during half-marathon running.Methods: Eighteen half-marathon runners completed two self-paced 21 km treadmill running trials, once wearing CRP and once wearing loose running shorts (LRS). For each 21 km run, RPE, heart rate, blood lactic acid, and knee flexion proprioception were assessed before starting, and after each 7 km stage.Results: Data analysis revealed no difference between CRP and LRS conditions in heart rate, RPE, or blood lactic acid. Repeated measures ANOVA showed a significant garment condition main effect whereby wearing CRP was associated with higher knee proprioceptive acuity (p = 0.006). Polynomial trend analysis showed a significant linear downwards trend in proprioceptive acuity across the four measurement occasions (p = 0.048). Stage analysis showed that wearing CRP was associated with better knee proprioception at running distances of 14 km (p = 0.007, 95%CI = -0.054, -0.010) and 21 km (p = 0.016, 95%CI = -0.051, -0.006).Conclusion: Compression running pants provide an overall positive effect on knee proprioception, particularly after 14 km and 21km, which may reduce the probability of knee injury. CRP had no significant effect on physiological measures in half-marathon running.
Mediator role of presence of meaning and self-esteem in the relationship of social support and death anxiety
Authors: Huang Yuxin , Guan Ziyao , Yan Fang , Wiley James A. , Reynolds Nancy R. , Tang Siyuan , Sun Mei 
Published: December 2022

IntroductionDeath anxiety has increased following the COVID-19 pandemic. Although terror management theory has suggested social support, presence of meaning and self-esteem functioned as death anxiety buffers, few existing works have explored the mechanism of how social support, presence of meaning, and self-esteem buffer death anxiety. To identify these mechanisms is the aim of this study.MethodsOur cross-sectional study was conducted with 1167 people in China from 19 May 2020 to 1 June 2020 during the COVID-19 outbreak. The average age of participants was 26 years. Data were by questionnaire, including demographic information, the Templer's Death anxiety scale, the multidimensional scale of perceived social support, the presence of meaning scale, and the Rosenberg self-esteem scale.ResultsResults using structural equation modeling showed presence of meaning and self-esteem fully mediated the relationship between social support and death anxiety, respectively and sequentially. The proposed model showed good fit of indices: χ2 = 243.384, df = 58, p &lt; 0.001; CFI = 0.968, TLI = 0.954, RMSEA = 0.052, SRMR = 0.044.DiscussionThis study demonstrates significant mediator roles of presence of meaning and self-esteem in the relationship of social support and death anxiety. Multi-component interventions are needed to manage death anxiety by targeting increasing social support, presence of meaning and self-esteem and increasing presence of meaning and self-esteem when social support is diminished in the pandemic.