bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new platform for the post-functionalization of porous polymer based on calix[4]resorcinarene under thermal solvent-free conditions; as a highly efficient and recyclable PTC containing nanocavity
Authors: Elahi Somayeh , Khodadoost Mehrdad , Shahidizadeh Elham 
Published: August 2022

Abstract
In this work, for the first time, functionalized 3D-network polymer was synthesized through thermal solvent-free method. The synthetic steps include (1) the preparation of ionic liquid (1-methyl-3-(trimethoxysilylpropyl) imidazolium choloride) [pmim]Cl, (2) synthesis of the 3D-network polymer based on calix[4]resorcinarene, and finally (3) immobilization of ionic liquid on the synthesize polymer through one-pot solvent-free conditions. The formation of this cationic porous polymer was confirmed by elemental analysis, scanning electron microscopy (SEM), thermal gravimetric analysis (TGA) and derivative thermogravimetric (DTG). Taken together, the results reveal the successful preparation of the efficient and wondrous mesoporous cationic polymer with the simultaneously merged properties of ionic liquid and polymeric matrix on the basis of calix[4]resorcinarene. Therefore, it provides an efficient heterogeneous catalyst for being applied in the catalytic reaction for achieving synthetic goals. One-pot conditions, thermal solvent-free pathway, easy reaction handling and work up, green and affordable methodology, gaining high yields of product are the most important advantages of the present method for the synthesis of this polymeric heterogeneous catalyst. The hydrophobic central cavity and hydrophilic cationic arms render it a suitable heterogeneous phase transfer catalyst for the preparation of valuable azidohydrins in water. The recovered catalyst could be used for several times without any significant loss of activity. The unique features of this catalyst such as superior thermal stability, excellent catalytic activity in terms of yield and reaction time, will be potentially important for its applications in industry.
Elucidation of adipogenic differentiation regulatory mechanism in human maxillary/mandibular bone marrow-derived stem cells
Authors: Miyata Haruka , Ishii Masakazu , Suehiro Fumio , Komabashiri Naohiro , Ikeda Nao , Sakurai Tomoaki , Nishimura Masahiro 
Published: August 2022

Abstract
Mesenchymal stem cells (MSCs) are pluripotent stem cells present in various tissues. Previous studies have shown that maxillary/mandibular bone marrow-derived MSCs (MBMSCs) scarcely differentiate into adipocytes. However, the regulatory molecular mechanisms underlying adipogenic differentiation of MBMSCs remain unknown. We investigated the underlying molecular mechanisms that regulate adipogenic differentiation of MBMSCs. We observed no significant differences in cell surface antigen profiles and stem cell marker gene expression in MBMSCs and iliac bone marrow-derived MSCs (IBMSCs). MBMSCs and IBMSCs displayed similar osteogenic and chondrogenic differentiation potentials, whereas MBMSCs showed significantly lower lipid accumulation, adipocyte marker gene expression, and intracellular glucose uptake than that showed by IBMSCs. Expression of CCAAT/enhancer binding protein β (C/EBPβ), C/EBPδ, early B-cell factor 1 (Ebf-1), and Krüppel-like factor 5 (KLF5), which are transcription factors expressed early in adipogenic differentiation, was suppressed in MBMSCs compared to that in IBMSCs. Peroxisome proliferator-activated receptor-γ (PPARγ) and C/EBPα, which play important roles in the terminal differentiation of preadipocytes into mature adipocytes, were increased during adipogenic differentiation in MBMSCs and IBMSCs; however, the expression level of these genes in MBMSCs was lower than that in IBMSCs. Furthermore, the level of zinc finger protein 423 (Zfp423), which is involved in the commitment of undifferentiated MSCs to the adipocyte lineage, was significantly lower in undifferentiated MBMSCs than that in IBMSCs. These data indicate that MBMSCs are negatively regulated in the commitment of undifferentiated MSCs to the adipocyte lineage (preadipocytes) and in terminal differentiation into mature adipocytes. These results may elucidate the site-specific characteristics of MBMSCs.
Validation of an analytical model of groundwater velocity based on laboratory test and numerical simulation
Authors: Ren Jie , Zhang Jinjin , Wang Dabo 
Published: August 2022

Abstract
Surface water-groundwater exchange affects the material and energy transfer of rivers and adjacent riparian zones. As an intuitive carrier of energy, temperature can effectively reflect the Spatio-temporal changes the surface water-groundwater exchange process. In this paper, the influence of water head variation and sand sample uniformity on its temperature field and seepage field is studied through a one-dimensional sand column laboratory test. Compare the vertical seepage velocities measured in laboratory tests with the vertical underflow exchange rate calculated by the four analytical models to verify the accuracy of the one-dimensional vertical heat analytical model. The results show that the Hatch analytical solution, Keery analytical solution, McCallum analytical solution and Luce analytical solution calculated by VFLUX2 through MATLAB are reliable for calculating the vertical undercurrent exchange rate of the heterogeneous sand column.
CircFAM188A regulates autophagy via miR-670-3p and ULK2 of epithelial ovarian carcinoma
Authors: Yong Min , Zeng Yuhua , Yao Yuqin , Yang Miyuan , Tang Furong , Zhu Hongtao , Zhou Heng , Hu Jianguo 
Published: August 2022

Abstract
Background: To detect the effect and mechanism of circFAM188A in autophagy, proliferation and invasion of ovarian cancer. Methods: RT-PCR was used to detect the expression of circFAM188A in epithelial ovarian cancer tissue. mRFP-GFP-LC3 reporter, EDU and transwell assay were used to analyze autophagy, proliferation and invasion. The binding site of circ-FAM188A and miR-670-3p was predicted by bioinformatics analysis and confirmed by dual-luciferase reporter gene assay. RNA-pulldown was used to find the protein directly combined with circ-FAM188A. Subcutaneous tumorigenesis model was conducted by subcutaneous injection in nude mice. Results: Circ-FAM188A expressed higher in EOC than in para-carcinoma tissue. Overexpression of circ-FAM188A promoted autophagy, proliferation and invasion, which were inhibited by silencing of circ-FAM188A. The colocalization of Circ-FAM188A and miR-670-3p was mainly in cytoplasm. circ-FAM188A upregulated the expression of YY1 via sponging miR-670-3p. Circ-FAM188A could directly combine with ULK2. Conclusions: Circ-FAM188A expressed highly in EOC tissue. Circ-FAM188A regulated autophagy via sponging miR-670-3p and directly combining with ULK2.
Fucosyltransferases Regulated by Fusobacterium Nucleatum Promote the Progression of Colon Adenocarcinoma
Authors: Wang Pengfei , Liu Xuxu , Yu Jingjing , Meng Ziang , Lv Zhenyi , Shang Ce , Geng Qi , Wang Dawei , Xue Dongbo , Li Long 
Published: August 2022

Abstract
Background
Colon adenocarcinoma (COAD) is one of the leading causes of cancer-associated mortality worldwide. Fucosyltransferases (FUTs) are associated with numerous cancers. We aimed to investigate the functions of FUTs in COAD.
Methods
Transcriptomic and clinical data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were used to analyze the expression and clinical relevance of FUTs in COAD. Real Time Quantitative PCR (RT-qPCR), Western blot, immunohistochemistry and ELISA were used to detect the relative RNA and protein expression levels. Colitis-associated cance mice treated with Fusobacterium nucleatum were used to illustrate the effects of Fusobacterium nucleatum on FUTs and COAD. Luciferase reporting assay was used to investigate the binding of miRNA to mRNA.
Results
TCGA and GEO datasets showed abnormal expression of FUTs in COAD at transcript level. RT-qPCR, Western blot and immunohistochemistry showed increased expression of FUT1, POFUT1 and POFUT2 in COAD. COAD patients with a high expression of FUT1, FUT11, FUT13 (POFUT2) had a worse prognosis, while patients with a high expression of FUT2, FUT3, FUT6 had a better prognosis. FUT1 and POFUT2 could independently predict the prognosis of COAD patients. Functional analysis by CancerSEA database showed that FUT3, FUT6, FUT8, FUT12 (POFUT1) and FUT13 are associated with differentiation, apoptosis, invasion, quiescence, and hypoxia. FUTs are associated with the tumor microenvironment of COAD. FUT1 regulated by miR-939-3p inhibit the expression of MUC2. Fusobacterium nucleatum may affect the expression of FUTs by affecting their transcription factors and miRNA levels. Moreover, Fusobacterium nucleatum promotes COAD progression through the miR-939-3p/FUT1/MUC2 axis.
Conclusions
Fucosyltransferases play an important role and may be the mediator of Fusobacterium nucleatum promoting COAD progression.
Endosialin positive tumor derived pericytes promote tumor progression through impeding the infiltration of CD8 + T cells in clear cell renal cell carcinoma
Authors: Lu Tong , Zhang Jiayu , Lu Shiqi , Yang Fa , Gan Lunbiao , Wu Xinjie , Liu Shaojie , Xu Chao , Han Donghui , Yang Bo , Wen Weihong , Qin Weijun , Yang Lijun 
Published: August 2022

Abstract
Background: Clear cell renal cell carcinoma (ccRCC) is considered to be an immunogenic tumor, and immune checkpoint blockade (ICB) therapy provides effective option for RCC treatment. However, a large proportion of patients do not benefit from ICB therapy. Tumor derived pericytes (TDPs) are an important component in tumor microenvironment (TME), and may promote tumor progression through the regulation on T cells. TDPs has been considered as ideal target for tumor treatment. However, like CAFs, TDPs are also heterogeneous, different subclusters may have distinct function. Here, we aimed to identify new specific marker for tumor promoting TDPs and to develop novel TDPs targeting therapeutic strategies to enhance the efficacy of ICB therapy in ccRCC.Methods: To analyze the relationship between endosialin (EN) expression and the infiltration of CD8+ T cells in ccRCC, we performed flow cytometry using freshly isolated tumor specimens from ccRCC patients. Then, their correlation was verified in ccRCC-bearing wildtype and endosialin knockout mice, and also by antibody mediated blockade of endosialin. RNA-sequencing (RNA-seq) using EN-high and EN-low TDPs from ccRCC tissues and published single-cell RNA-sequencing (scRNA-seq) data of ccRCC were analyzed to figure out the potential functions of EN-high TDPs in CD8+ T cell infiltration and tumor progression. Function of endosialin in the regulation of cell proliferation and migration of TDPs, and CD8+ T cells infiltration was examined in vitro. Combined treatment with endosialin antibody and PD-1 blockade was applied to treat ccRCC-bearing mice and the anti-tumor effect was examined.Results: We found that high endosialin expression was associated with low infiltration of CD8+ T cells in clinical ccRCC tissues. Endosialin knockout or antibody blockade could significantly increase the infiltration of CD8+ T cells in ccRCC-bearing model. RNA-seq and scRNA-seq analysis indicated that high EN-high TDPs represent an activated state of TDPs. We also demonstrated that endosialin could promote the proliferation and migration of TDPs and impede the infiltration of CD8+ T cells in vitro. At last, we showed that combined treatment with anti-endosialin antibody could enhance the anti-tumor efficacy of PD-1 blockade.Conclusion: We reported EN-high TDPs represent an activated state of TDPs and inhibit the infiltration of CD8+ T cells into ccRCC tissues. Combined treatment with anti-endosialin antibody and PD-1 antibody provided a rational strategy to improve effect of ICB therapy in ccRCC.
Arteritic anterior ischemic optic neuropathy and cilioretinal artery occlusion induced by giant cell arteritis in Chinese: two cases report
Authors: Jiang Zhijian , Ji Huiying , Zhu Maoli , Zhang Nan , Chang Qing , Dong Jianhong 
Published: August 2022

Abstract
Background: Vision loss in patients with arteritic anterior ischemic optic neuropathy (A-AION) caused by giant cell arteritis (GCA) is disastrous and GCA is very rare in Asian individuals. We report two Chinese patients presenting with A-AION complicated with cilioretinal artery occlusion (CLAO), and the temporal artery biopsy (TAB) proved the diagnose of GCA.Case presentation: Two elderly male patients presented with sudden vision loss in the right eyes. Fundus examination of the right eyes revealed chalky, white, swollen optic papilla and swollen region of the retina between the macular and the optic disc. OCT showed a thickened nerve fiber layer of the optic disc and an enhanced signal intensity of the nuclear layer between the macular and the optic disc in OCT. OCTA revealed a decreased density of capillaries around the optic disc and non-perfusion of the deep retina between the macula and the optic disc. The laboratory tests included elevated CRP and ESR. Histopathology of the right temporal artery revealed the infiltration of lymphocytes, plasma cells, histiocytes, and multinucleated giant cells. TAB confirmed the diagnosis of GCA-induced A-AION combined with CLAO. They were treated with intravenous methylprednisolone for 6 days and oral prednisone 1 mg/kg/d, which was gradually tapered. The visual acuities of right eyes were improved in both patients at 6 months follow-up.Conclusions: GCA can lead to A-AION combined with CLAO in Chinese patients. OCTA is a noninvasive and convenient procedure that can reveal decreased density of capillaries around the optic disc and non-perfusion of the deep retina between the macula and the optic disc through the detected of retinal microcirculation in patient with GCA-induced A-AION combined with CLAO.
Persistence of P. falciparum HRP-2 antigenaemia post treatment is not associated with gametocytes
Authors: Oulton Tate , Mahamar Almahamoudou , Sanogo Koualy , Diallo Makonon , Youssouf Ahamadou , Niambele Sidi M , Samaké Siaka , Keita Sekouba , Sinaba Youssouf , Sacko Adama , Traore Sekou F , Lanke Kjerstin , Collins Katharine , Bradley John , Drakeley Chris , Stone William , Dicko Alassane 
Published: August 2022

Abstract
Background
In some settings, sensitive field diagnostic tools may be needed to achieve elimination of falciparum malaria. To this end, rapid diagnostic tests (RDTs) based on the detection of the Plasmodium falciparum protein HRP-2 are being developed with increasingly lower limits of detection. However, it is currently unclear how parasite stages that are unaffected by standard drug treatments may contribute to HRP-2 detectability and potentially confound RDT results even after clearance of blood stage infection. In this study, we assessed the detectability of HRP-2 in periods of post-treatment residual gametocytaemia.
Methods
A cohort of 100 Plasmodium falciparum infected, gametocyte positive individuals were treated with or without the gametocytocidal drug primaquine (PQ), alongside standard artemisinin-based combination therapy (ACT), in the context of a randomised clinical trial in Ouelessebougou, Mali. We used a quantitative ELISA to measure levels of HRP-2, and compared time to test negativity using a standard and ultra-sensitive RDT (uRDT) between residual gametocyte positive and negative groups.
Findings
Time to test negativity was longest by uRDT, followed by ELISA and then standard RDT. No significant difference in time to negativity was found between the treatment groups with and without residual gametocytes: uRDT (HR 0.79 [95% CI 0.52–1.21], p = 0.28), RDT (HR 0.77 [95% CI 0.51–1.15], p = 0.20) or ELISA (HR 0.88 [95% CI 0.59–1.32], p = 0.53). Similarly, no difference was observed when adjusting for baseline asexual parasite density. Quantified levels of HRP-2 over time were similar between groups, with differences attributable to asexual parasite densities. Furthermore, no difference in levels of HRP-2 was found between individuals who were or were not infectious to mosquitoes (OR 1.19 [95% CI 0.98–1.46], p = 0.077).
Interpretation
Surviving sexual stage parasites after standard ACT treatment do not contribute to the persistence of HRP-2 antigenaemia, and appear to have little impact on RDT results.
Determination of multi-pesticides residues in jasmine flower and its scented tea
Authors: Zhou Yilu , Wu Yangliu , Zeng Wenbo , An Quanshun , Chen Guanyu , Pan Canping 
Published: August 2022

Abstract
For minor crops such as jasmine, the lack of pesticide registration and maximum residue limits are important issues that need to be solved in order to facilitate trading and ensure food safety. Meanwhile, reliable and quick analytical methods for multi-pesticide residues in these commodities are few, but required by various stakeholders. In this study, a method for detecting 25 of the most frequently used pesticides in jasmine flower and its scented tea by multi-plug filtration cleanup and ultra-high-performance liquid chromatography-tandem mass spectrometry was developed and validated. The cleanup process was optimized and compared with the dispersive solid phase extraction procedure. Results showed that the relative standard deviations of multi-plug filtration cleanup method in precision tests were smaller. The absence of any solvent evaporation, vortices and additional centrifugation processes made this approach more rapid. The method was validated, showing that except for methomyl, recoveries of 25 pesticides were 64–108%, with relative standard deviations (n = 5) of 0.33-10%. The method was successfully applied to detect pesticide residues in marketed jasmine flower and scented tea samples. The results showed that some samples contained a combination of different residues, with neonicotinoids, organophosphates and pyrethroids being the three main types detected in both flower and tea samples.
Cellular automata imbedded memristor-based recirculated logic in-memory computing
Authors: Liu Yanming , Tian He , Wu Fan , Liu Anhan , Li Yihao , Sun Hao , Lanza Mario , Ren Tian-Ling 
Published: August 2022

Abstract
Cellular automata is an important tool to study the emergent properties of complex systems based on its well-known parallel, bio-inspired, computational characteristics. However, running cellular automata on conventional chips suffer from low parallelism, and high hardware cost. Establish dedicated hardware for cellular automata remains elusive. Here, we propose a recirculate logic operations scheme (RLOS) based on memristive hardware combined with 2D transistors to realize cellular automata evolution. The scheme utilizes the storage and calculation characteristics of memristive devices, which greatly reduces hardware complexity. The versatility of the RLOS scheme allows implementing multiple different cellular automata algorithms on the same circuitry. The entire rule (rule 1-254) of elementary cellular automata and more complicated 1D CA model majority classification algorithm have been verified to be applicable to this circuitry. Further, the edge detection algorithm based on 2D cellular automata has been authenticated through RLOS. The experimental and evaluation results reveal that the scheme reduces the hardware cost up to 79 times comparing to the Field Programmable Gate Array (FPGA) approach. To our best knowledge, RLOS has the lowest hardware cost (6 components/per cell) among state-of-art hardware implementations. This work can pave the road towards high-efficiency and low-cost cellular automata hardware realization, and also facilitates the exploration of memristive applications.
Identification of the driving mechanisms associated with aggressive transformation and prognosis in follicular lymphomas
Authors: Li Xinglong , Wang Run , Chen Xianjin , Yu Yang , He Changchun , Zhang Qinghua 
Published: August 2022

Abstract
Follicular lymphoma (FL) is the most frequently common indolent subtype of non-Hodgkin’s lymphoma (NHL), with a generally favorable prognosis. Recent studies have elucidated a subset of patients undergoing histological transformations (HTs) into transformed follicular lymphoma (tFL) and diffuse large B-cell lymphoma (DLBCL) aggressive morphology, leading to a poorer prognosis. Investigating the mechanisms of oncogenic transformation further could be helpful to find potential prognostic biomolecular markers that could be used to develop new treatment strategies or combination strategies. In this study, we conducted integrative bioinformatics analyses of the GEO, DAVID, STRING, GeneMANIA, TRRUST, and DGIdb database to determine which hub genes contributed to aggressive HTs of FL. The results show that HTs-related 462 and 503 differential genes contained between tFL and FL as well as between DLBCL and FL, respectively. Further functional enrichment analysis of these differential genes showed that the common significant enrichment in cell division, immune response, mitotic spindle organization, chromosome segregation of biological processes (BPs), extracellular space, extracellular region, and external side of the plasma membrane of cellular components (CCs), protein binding, microtubule-binding, heparin-binding, extracellular matrix structural constituent, and integrin binding of molecular functions (MFs), and cytokine-cytokine receptor interaction, chemokine signaling pathway, and viral protein interaction with cytokine and cytokine receptor signaling pathway of KEGG pathways. Subsequently, construct an overlapping PPI network to screen 10 hub genes (CCNB1, AURKA, RRM2, CDK1, BUB1B, CDC6, ASPM, TTK, TPX2, and NCAPG). We then analyzed the transcription factor regulation and drug regulatory networks. In summary, our study identified 3 oncogenic transformation molecules (CDK1, RRM2, and AURKA) as important biomarkers in the progression of FL. Their abnormal expression is closely associated with prognosis and sensitivity to standard therapy and can help us better understand the aggressive HTs of FL.
Identification of climatic and management factors influencing wheat’s yield variability using AgMERRA dataset and DSSAT model across a temperate region
Authors: Farhadi Masume , Bannayan Muhammad , Fallah Muhammad Hassan , Jahan Mohsen 
Published: August 2022

Abstract
One of the main challenges of current agriculture to ensure food security is the development of strategies to deal with potential negative impacts and adaptation to climate variability. This study has conducted to determine climatic and management factors influencing wheat yield variability throughout a temperate region in Northeastern Iran in the period of 1980–2010. The growth stages and yield of wheat crop were simulated with DSAAT model, using AgMERRA gridded dataset and the effect of climatic variables on yield was identified using Panel Data Regression (PDA). According to the results, 63% of the changes in irrigated wheat yield are explained by environmental factors (temperature and precipitation) and 37% by management factors. PDA revealed that among the climatic variables, the number of temperatures above 30 oC during the growing season, mean temperature, amount and frequency of precipitation have a significant effect on irrigated wheat yield (p ≤ 0.05). The length of wheat growing season throughout the study region were decreased by 26 days during the study period. The management practices, including the provision of inputs such as chemical fertilizers, modified seeds, tillage machinery and equipment, information transfer and the penetration of knowledge in the field, increase yields by averaged if 5 kg per year in study region. In general, employing the effective management methods, in particular selecting the appropriate planting date that could be resulted in better adaptation of the phenological stages of wheat to environmental conditions, can improve wheat yield. The results of this research indicate that using valid AgMERRA meteorological dataset as input for DSSAT crop model could produce reliable simulations which in turn could be employed by food policy and decision makers, farmers and managers in a temperate region.