bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case Report: Giant Congenital Left Atrial Appendage Aneurysm Presenting With Acute Massive Cerebral Infarction and Refractory Atrial Fibrillation: A Case Report and Literature Review
Authors: Li Rui , Ma Fei , Guan Han Xiong , Pan Yue Ying , Liu Li Gang , Wang Dao Wen , Wang Hong 
Published: May 2022

BackgroundCongenital left atrial appendage aneurysm (LAAA) is a rare cardiac anomaly with a variety of presentations, from being asymptomatic to potentially serious complications such as systemic thromboembolism and atrial tachyarrhythmia.Case PresentationWe report a case of congenital giant LAAA in a 35-year-old man presenting with acute massive cerebral infarction and atrial fibrillation (AF) with rapid ventricular rate. The AF was refractory to conventional antiarrhythmic agents, such as amiodarone and electrical cardioversion, but restored and maintained sinus rhythm after surgical resection of LAAA. The patient remained free of events and was in sinus rhythm during half-year follow-up.ConclusionGiant LAAA has the potential causing serious complications and should be managed surgically in most cases.
Analysis of the Thermogenic Gas Source of Natural Gas Hydrates Over the Dongsha Waters in the Northern South China Sea
Authors: Feng Changmao , Zhong Guangjian , Sun Ming , Lei Zhenyu , Yi Hai , Zhao Zhongquan 
Published: May 2022

Gas hydrates were newly discovered in the Dongsha Waters of the South China Sea by drilling in 2013 and 2019. The drilling results show that the hydrates consist of microbial gas and thermogenic gas, but the source layers of the thermogenic gas are not clear. The Chaoshan depression is the largest relict Mesozoic basin under the Dongsha Waters where thick T3—K strata, up to 5000 m, have developed under thin Cenozoic sediments. The T3—J1 and J3 bear the two main layers of hydrocarbon source rocks rich in II2-III type organic matter, mainly in the western sag, the middle low bulge, and the eastern sag. The T3—J1 developed bathyal - neritic mud rocks, generally greater than 400 m in thickness. The J3 mainly developed closed and semi-closed bay type mud, also II2-III type. Nowadays these two layers are in their mature - over mature stages, thus potentially high-producing. There have developed deep faults cutting through the Mesozoic strata and distributing fractures within the Cenozoic sediments. Analysis of geochemical and geological samples dredged from the seabed indicates a significant oil and gas leakage activity well correlated spatially with the Mesozoic-associated faults. The association of fault-fractures can act as good channels for the thermogenic gas to move upward and diffuse into the temperature pressure stabilization zone of the hydrate. Thus, sufficient gas from the Mesozoic production layers is deemed the main source of thermogenic gas. The area where the Mesozoic faults are activated is the main leakage area of thermogenic gas, thus the main target for future hydrate exploration.
Sleep Behavior and Self-Reported Infertility: A Cross-Sectional Analysis Among U.S. Women
Authors: Liang Zhu , Liu Jianqiao 
Published: May 2022

ObjectiveTo investigate the associations between sleep behaviors and female infertility.MethodsWe conducted a cross-sectional study composed of 2175 U.S. women 18-44 years of age from the National Health and Nutrition Examination Survey (NHANES) (2015-2018). Bedtime/waketime and sleep duration were extracted from the sleep disorder questionnaire. Self-reported infertility was defined as a binary variable based on the participants’ response to the question, “Have you ever attempted to become pregnant over a period of at least a year without becoming pregnant?”. Multivariate logistic regression analyses were done to explore the relationship between sleep behaviors and female infertility.ResultsBedtime (OR=1.24; 95% CI, 1.10-1.40, P = 0.001) and waketime (OR=1.14; 95% CI, 1.01-1.28, P = 0.037) were associated with infertility. Waketime of 08:00 was the inflection point, above which the probability of infertility increased rapidly (OR=1.41; 95% CI, 1.11-1.79, P = 0.004). Sleep-wake behavior was significantly associated with infertility (OR=1.34; 95% CI, 1.16-1.53, P &lt; 0.001) and participants with early-bed/early-rise behavior had the lowest risk.ConclusionsAmong U.S. women 18-44 years of age, bedtime and waketime were significantly linearly and non-linearly correlated with infertility, respectively. Early-bed/early-rise behavior was associated with the lowest infertility rate. Further study is needed because the timing of sleep behaviors are modifiable factors and could be a novel strategy to cope with infertility.
Correlation Between Thyroid Hormone Concentrations and Ultrasound Thyroid Volume in Preterm Infants Born Before 33 Weeks of Gestation
Authors: Mikołajczak Aleksandra , Kufel Katarzyna , Bokiniec Renata 
Published: May 2022

ObjectiveThyroid disorders are commonly concomitant with premature birth; however, indications to start therapy remain unclear due to a lack of gestational age (GA)-specific reference ranges. We aimed to evaluate the age-specific thyroid-stimulating hormone (TSH), free thyroxine (FT4) levels and the correlation between TSH and FT4 serum levels and ultrasound thyroid volume in preterm infants.Materials and MethodsThis was an observational, prospective, single-center study of 98 preterm infants born before 33 weeks GA. The infants were divided into the 24-28 weeks and 29-32 weeks GA groups. TSH and FT4 serum levels were measured at two time points: at postnatal age (PNA) 2 weeks and at postmenstrual age (PMA) 32 weeks; the results were compared between groups at two consecutive time points.ResultsThere was a statistically significant between-group difference in FT4 concentration. There was a positive correlation between FT4 and GA at both screening times. FT4 in the 24-28 weeks GA group was significantly lower than in the 29-32 weeks GA group. The mean (standard deviation [SD]) FT4 at PNA 2 weeks was 11.72 ± 2.16 pmol/l for the 24-28 weeks GA group vs. 13.33 ± 1.80 pmol/l for the 29-32 weeks GA group (p&lt;0.001). The mean (SD) FT4 at PMA 32 weeks was 11.96 ± 1.98 pmo/l for the 24-28 weeks GA group vs. 13.33 ± 1.80 pmol/l for the 29-32 weeks GA group (p=0.001). Our results reflect a slow and gradual upward trend of FT4 in the 24-28 weeks GA. It is of interest that the correlation between thyroid volume and FT4 was statistically significant (rho=0.25, p=0.019) for all studied preterm infants. The correlation between thyroid volume and weight was statistically significant for the entire study group (rho=0.37, p&lt;0.001). We did not find statistically significant differences in TSH and FT4 values between consecutive time points at 24-28 weeks GA. The thyroid volume was not significantly different between both groups. The total thyroid volume was 0.26 vs. 0.27 ml for the 24-28 and 29-32 weeks GA groups, respectively.ConclusionThe results of this study indicate that preterm infants require lower FT4 values depending on GA. Moreover, ultrasound thyroid imaging may facilitate the evaluation of questionable thyroid disorders.
Hsa_circ_0062270 Promotes Tumorigenesis of Melanoma by Stabilizing the Linear Transcript Cell Division Cycle Protein 45
Authors: Wei Cuie , Sun Wentao , Liu Changhai , Meng Fanjun , Sun Lele , Ding Xiangsheng 
Published: May 2022

Background: To elucidate the potential biological function of hsa_circ_0062270 in the malignant process of melanoma and its potential target.Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to examine relative level of hsa_circ_0062270 in melanoma tissues and normal skin tissues. The diagnostic and prognostic potentials of hsa_circ_0062270 in melanoma were evaluated. The regulatory effect of hsa_circ_0062270 on the expression of linear transcript Cell division cycle protein 45 (CDC45) was also examined.Results: Hsa_circ_0062270 was up-regulated in melanoma samples and cell lines, which displayed certain diagnostic and prognostic potentials in melanoma. Inhibition of hsa_circ_0062270 attenuated the proliferative, migratory and invasive functions. Hsa_circ_0062270 could stabilize the expression of linear transcript CDC45, and thus participated in the malignant process of melanoma.Conclusion: Hsa_circ_0062270 promotes proliferative, migratory and invasive functions of melanoma cells via stabilizing the linear transcript CDC45. Hsa_circ_0062270 can be used to diagnosis and treatment of melanoma.
Household Structure and Contraceptive Use in Nigeria
Authors: Fadeyibi Opeyemi , Alade Mayowa , Adebayo Samuel , Erinfolami Temitope , Mustapha Fatimah , Yaradua Saudatu 
Published: May 2022

BackgroundContraceptive use in Nigeria has been consistently low despite its many benefits and several efforts by government and development partners to increase its uptake. According to the Nigeria Demographic and Health Survey, the use of any modern method staggeringly increased from 4 to 12% over 28 years (1990–2018). Studies have identified factors at the individual, household, and societal levels that affect contraceptive use. While studies have also shown that decisions such as contraceptive behavior and acquisition of family skills may vary according to the individual or societal factors, there is a dearth of knowledge on how household structure and composition influence contraceptive use in Nigeria. This article seeks to contribute to the body of knowledge by exploring contraceptive use within the household context.MethodWe used data from the 2018 Nigeria Demographic and Health Survey to examine the relationship between household structure and contraceptive use. We excluded pregnant and non-married women at the time of the survey from the sample and used multinomial regression analysis to examine the likelihood of using traditional or modern methods of contraception.ResultsResults show that having a large household size, and the presence of multiple wives in the household significantly reduces the likelihood of using any method of contraceptive. The result further shows a significant association between household wealth index and contraceptive use as the use of any method increases with household wealth index, with those from richest households being twice as likely as their counterparts in the poorest households to use traditional methods (OR:2.02, p &lt; 0.05). Also, women living in households headed by older men (25 and above), households with under 5 children, and those living in rural areas have significantly reduced likelihood of using any method.ConclusionThis study highlights the dynamics of contraceptive use among married women considering household composition. While our study serves as a primer to understanding contraceptive use in households where a woman and her spouse are usual household members, improved family planning interventions to increase uptake through demand creation will require deeper and more comprehensive work to understand the dynamics among women in more complex household settings.
The Effect of Peripheral Immune Cell Counts on the Risk of Multiple Sclerosis: A Mendelian Randomization Study
Authors: He Di , Liu Liyang , Shen Dongchao , Zou Peng , Cui Liying 
Published: May 2022

ObjectivesMultiple sclerosis (MS) is a complex central nervous system (CNS) demyelinating disease, the etiology of which involves the interplay between genetic and environmental factors. We aimed to determine whether genetically predicted peripheral immune cell counts may have a causal effect on MS.MethodsWe used genetic variants strongly associated with cell counts of circulating leukocyte, lymphocyte, monocyte, neutrophil, eosinophil, and basophil, in addition to some subpopulations of T and B lymphocyte, as instrumental variables (IVs) to perform Mendelian randomization (MR) analyses. The effect of immune cell counts on MS risk was measured using the summary statistics from the International Multiple Sclerosis Genetics Consortium (IMSGC) genome-wide association studies (GWAS).ResultsOur findings indicated that higher leucocyte count [odds ratio (OR), 1.24; 95% confidence interval (CI), 1.07 - 1.43; p = 0.0039] and lymphocyte count (OR, 1.17; 95% CI, 1.01 – 1.35; p = 0.0317) were causally associated with MS susceptibility. In addition, we also found that increase of genetically predicted natural killer T (NKT) cell count is also associated with an increase MS risk (OR, 1.24; 95% CI, 1.06 - 1.45; p = 0.0082).ConclusionsThese findings show that the genetic predisposition to higher peripheral immune cell counts can exert a causal effect on MS risk, which confirms the crucial role played by peripheral immunity in MS. Particularly, the causal association between NKT cell count and MS underscores the relevance of exploring the functional roles of NKT cells in disease pathogenesis in future.
Clinical Significance of a CD3/CD8-Based Immunoscore in Neuroblastoma Patients Using Digital Pathology
Authors: Zeng Liang , Li Shu-Hua , Xu Shuo-Yu , Chen Kai , Qin Liang-Jun , Liu Xiao-Yun , Wang Fang , Fu Sha , Deng Ling , Wang Feng-Hua , Miao Lei , Li Le , Liu Na , Wang Ran , Wang Hai-Yun 
Published: May 2022

BackgroundInfiltrating immune cells have been reported as prognostic markers in many cancer types. We aimed to evaluate the prognostic role of tumor-infiltrating lymphocytes, namely CD3+ T cells, CD8+ cytotoxic T cells and memory T cells (CD45RO+), in neuroblastoma.Patients and MethodsImmunohistochemistry was used to determine the expression of CD3, CD8 and CD45RO in the tumor samples of 244 neuroblastoma patients. We then used digital pathology to calculate the densities of these markers and derived an immunoscore based on such densities.ResultsDensities of CD3+ and CD8+ T cells in tumor were positively associated with the overall survival (OS) and event-free survival (EFS), whereas density of CD45RO+ T cells in tumor was negatively associated with OS but not EFS. An immunoscore with low density of CD3 and CD8 (CD3-CD8-) was indictive of a greater risk of death (hazard ratio 6.39, 95% confidence interval 3.09-13.20) and any event (i.e., relapse at any site, progressive disease, second malignancy, or death) (hazard ratio 4.65, 95% confidence interval 2.73-7.93). Multivariable analysis revealed that the CD3-CD8- immunoscore was an independent prognostic indicator for OS, even after adjusting for other known prognostic indicators.ConclusionsThe new immunoscore based on digital pathology evaluated densities of tumor-infiltrating CD3+ and CD8+ T cells contributes to the prediction of prognosis in neuroblastoma patients.
Dynamic Habitat Models Reflect Interannual Movement of Cetaceans Within the California Current Ecosystem
Authors: Becker Elizabeth A. , Forney Karin A. , Miller David L. , Barlow Jay , Rojas-Bracho Lorenzo , Urbán R Jorge , Moore Jeff E. 
Published: May 2022

The distribution of wide-ranging cetacean species often cross national or jurisdictional boundaries, which creates challenges for monitoring populations and managing anthropogenic impacts, especially if data are only available for a portion of the species’ range. Many species found off the U.S. West Coast are known to have continuous distributions into Mexican waters, with highly variable abundance within the U.S. portion of their range. This has contributed to annual variability in design-based abundance estimates from systematic shipboard surveys off the U.S. West Coast, particularly for the abundance of warm temperate species such as striped dolphin,Stenella coeruleoalba, which increases off California during warm-water conditions and decreases during cool-water conditions. Species distribution models (SDMs) can accurately describe shifts in cetacean distribution caused by changing environmental conditions, and are increasingly used for marine species management. However, until recently, data from waters off the Baja California peninsula, México, have not been available for modeling species ranges that span from Baja California to the U.S. West Coast. In this study, we combined data from 1992–2018 shipboard surveys to develop SDMs off the Pacific Coast of Baja California for ten taxonomically diverse cetaceans. We used a Generalized Additive Modeling framework to develop SDMs based on line-transect surveys and dynamic habitat variables from the Hybrid Coordinate Ocean Model (HYCOM). Models were developed for ten species: long- and short-beaked common dolphins (Delphinus delphis delphis and D. d. bairdii), Risso’s dolphin (Grampus griseus), Pacific white-sided dolphin (Lagenorhynchus obliquidens), striped dolphin, common bottlenose dolphin (Tursiops truncatus), sperm whale (Physeter macrocephalus), blue whale (Balaenoptera musculus), fin whale (B. physalus), and humpback whale (Megaptera novaeangliae). The SDMs provide the first fine-scale (approximately 9 x 9 km grid) estimates of average species density and abundance, including spatially-explicit measures of uncertainty, for waters off the Baja California peninsula. Results provide novel insights into cetacean ecology in this region as well as quantitative spatial data for the assessment and mitigation of anthropogenic impacts.
Intensive Care Unit-Acquired Weakness in Patients With Extracorporeal Membrane Oxygenation Support: Frequency and Clinical Characteristics
Authors: Chen Xinyi , Lei Xiong , Xu Xin , Zhou Yu , Huang Man 
Published: May 2022

BackgroundIntensive care unit-acquired weakness (ICU-AW) is common in critical illness patients and is well described. Extracorporeal membrane oxygenation (ECMO) is used as a life-saving method and patients with ECMO support often suffer more risk factors of ICU-AW. However, information on the frequency and clinical characteristics of ICU-AW in patients with ECMO support is lacking. Our study aims to clarify the frequency and characteristics of ICU-AW in ECMO patients.MethodsWe conducted a retrospective study, ICU-AW was diagnosed when patients were discharged with a Medical Research Council (MRC) sum score &lt;48. Clinical information was collected from the case report forms. Univariable analysis, LASSO regression analysis, and logistic regression analysis were used to analyze the clinical data of individuals.ResultsIn ECMO population, 40 (80%) patients diagnosed with ICU-AW. On univariable analysis, the ICU-AW group had higher Acute Physiology and Chronic Health Evaluation II (APACHE II) [13.9 (6.5–21.3) versus 21.1 (14.3–27.9), p = 0.005], longer deep sedation time [2 (0–7) versus 6.5 (3–11), p = 0.005], longer mechanical ventilation time [6.8 (2.6–9.3) versus 14.3 (6.6–19.3), p = 0.008], lower lowest albumin [26.7 (23.8–29.5) versus 22.1 (18.5–25.7), p &lt; 0.001]. The LASSO analysis showed mechanical ventilation time, deep sedation time, deep sedation time during ECMO operation, APACHE II, and lowest albumin level were independent predictors of ICU-AW. To investigate whether ICU-AW occurs more frequently in the ECMO population, we performed a 1:1 matching with patients without ECMO and found there was no difference in the incidence of ICU-AW between the two groups. Logistic regression analysis of combined cohorts showed lowest albumin odds ratio (OR: 1.9, p = 0.024), deep sedation time (OR: 1.9, p = 0.022), mechanical ventilation time (OR: 2.0, p = 0.034), and APACHE II (OR: 2.3, p = 0.034) were independent risk factors of ICU-AW, but not ECMO.ConclusionThe ICU-AW was common with a prevalence of 80% in the ECMO population. Mechanical ventilation time, deep sedation time, deep sedation time during ECMO operation, APACHE II, and lowest albumin level were risk factors of ICU-AW in ECMO population. The ECMO wasn’t an independent risk factor of ICU-AW.
Impact of the COVID-19 Outbreak on Disease Spectrum of Pediatric Intensive Care Units
Authors: Li Wen Jing , Xue Chun Ling , Li Zhuo 
Published: May 2022

PurposeWe aimed to analyze the changes in the disease spectrum data of a pediatric intensive care unit (PICU) in Nanjing, China, during the COVID-19 outbreak and explore a feasible plan for the treatment of critically ill children.MethodsThis retrospective study used data from our PICU from 1 January 2018 to 31 December 2020. Patient demographics, distribution of disease spectrum, results of etiological examinations, and the PICU length of stay (LOS) were compared during the COVID-19 period (2020) and the previous years (2018 and 2019).ResultsIn 2020, the number of PICU admissions was 46.8 and 47.8% lower than that in 2018 and 2019, respectively. There were significant differences in the number of patients in PICU among different age groups, and these differences were mainly found in children aged &lt;4 years and older than 14 years. The percentage of the number of patients in PICU with respiratory diseases decreased significantly, while those with hematological diseases, poisoning, and rare diseases increased significantly. Moreover, the number of patients with rare diseases increased significantly, while the number of patients with mitochondrial diseases exceeded that of those with autoimmune encephalitis. The PICU LOS in 2020 was higher than that observed in 2018 and 2019, indicating that the changes in the PICU disease spectrum did not directly affect the PICU LOS. Etiological examinations revealed that during the COVID-19 period, the number of patients in PICU with bacterial infections increased, and those with viral infections decreased, although not statistically significant.ConclusionsA striking decrease in the number of PICU admissions was observed during the COVID-19 outbreak, which caused a significant change in the PICU disease spectrum. Changes in the number and characteristics of patients admitted to PICUs should be considered for facilitating the effective working of PICUs during the COVID-19 pandemic.
The Impact of Active Screening and Management on COVID-19 in Plateau Region of Sichuan, China
Authors: Lu Jia-jie , Jiang Guo-guo , He Xiang , Xu Kai-ju , Yang Hong , Shi Rui , Chen Ying , Tan Yu-yao , Bai Lang , Tang Hong , Li Guo-ping 
Published: May 2022

BackgroundIn December 2019, the cases of pneumonia of unknown etiology emerged in Wuhan, China, and rapidly spread throughout the country. The disease was later designated by the World Health Organization (WHO) as Coronavirus Disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS CoV-2). Few studies have assessed the clinical characteristics of COVID-19 and control strategies used to mitigate disease spread in high-altitude plateau regions of China.Study ObjectiveTo assess the impact of real-world strategies to control COVID-19 spread in remote plateau regions.MethodsA retrospective study was performed to assess the epidemiology of COVID-19 and strategies used to control disease spread in the high-altitude plateau of Sichuan, China from 24 January 2020 to 19 March 2020.ResultsCOVID-19 spread and outbreaks in Sichuan were attributed to mass gatherings. A total of 70 patients and 20 asymptomatic individuals were found in the hypoxic plateau region of Sichuan. Twelve patients were admitted after the onset of symptoms, while 58 patients and 20 asymptomatic individuals were found by active screening. The symptomatic patients included those with uncomplicated illness (16/70, 22.9%), mild pneumonia (44/70, 62.9%), and severe pneumonia (10/70, 14.3%). Most patients in the study area showed relatively mild and atypical symptoms such as low or no fever and dyspnea. The incidence of severe pneumonia, fever, dyspnea, and interstitial abnormalities identified by chest CT were all significantly lower in screened patients than those admitted after symptom onset (P &lt; 0.05). Severe pneumonia was noted in patients with chronic conditions like hypertension, diabetes etc. as compared to less severe pneumonia in healthy subjects (P &lt;0.05). No patients died and all were eventually discharged.ConclusionMass gatherings increased risk of spread of SARS-CoV-2 responsible for COVID-19. Active screening and early management have collectively contributed to reduced incidence of severe pneumonia and satisfactory prognoses of infections with COVID-19 in this hypoxic plateau region.