bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Effective Sodium-Dependent Glucose Transporter 2 Inhibition, Canagliflozin, Prevents Development of Hypertensive Heart Failure in Dahl Salt-Sensitive Rats
Authors: He Lili , Ma Sai , Zuo Qingjuan , Zhang Guorui , Wang Zhongli , Zhang Tingting , Zhai Jianlong , Guo Yifang 
Published: March 2022

Background: The aim of the study was to investigate the protective effect of canagliflozin (CANA) on myocardial metabolism and heart under stress overload and to further explore its possible molecular mechanism.Methods: High-salt diet was used to induce heart failure with preserved ejection fraction (HFpEF), and then, the physical and physiological indicators were measured. The cardiac function was evaluated by echocardiography and related indicators. Masson trichrome staining, wheat germ agglutinin, and immunohistochemical staining were conducted for histology analysis. Meanwhile, oxidative stress and cardiac ATP production were also determined. PCR and Western blotting were used for quantitative detection of related genes and proteins. Comprehensive metabolomics and proteomics were employed for metabolic analysis and protein expression analysis.Results: In this study, CANA showed diuretic, hypotensive, weight loss, and increased intake of food and water. Dahl salt-sensitive (DSS) rats fed with a diet containing 8% NaCl AIN-76A developed left ventricular remodeling and diastolic dysfunction caused by hypertension. After CANA treatment, cardiac hypertrophy and fibrosis were reduced, and the left ventricular diastolic function was improved. Metabolomics and proteomics data confirmed that CANA reduced myocardial glucose metabolism and increased fatty acid metabolism and ketogenesis in DSS rats, normalizing myocardial metabolism and reducing the myocardial oxidative stress. Mechanistically, CANA upregulated p-adenosine 5′-monophosphate-activated protein kinase (p-AMPK) and sirtuin 1 (SIRT1) and significantly induced the expression of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1a).Conclusion: CANA can improve myocardial hypertrophy, fibrosis, and left ventricular diastolic dysfunction induced by hypertension in DSS rats, possibly through the activation of the AMPK/SIRT1/PGC-1a pathway to regulate energy metabolism and oxidative stress.
Identification and Determination of Dimensions of Health-Related Quality of Life for Cancer Patients in Routine Care – A Qualitative Study
Authors: Schrage Theresa , Görlach Mirja , Schulz Holger , Bleich Christiane 
Published: March 2022

PurposeContinuous patient-reported outcomes (PROs) to identify and address patients’ needs represent an important addition to current routine care. The aim of this study was to identify and determine important dimensions of health-related quality of life (HrQoL) in routine oncological care.MethodsIn a cross-sectional qualitative study, interviews and focus groups were carried out and recorded. The interviewees were asked for their evaluation on HrQoL in general and specifically regarding cancer treatment. The material was transcribed and analyzed using qualitative content analysis based on Mayring. The results were reviewed in an expert discussion.ResultsInterviews with patients (N = 28) and clinicians (N = 4), as well as five focus groups with clinicians (N = 18) were conducted. Initially, nine deductive and two inductive categories on HrQoL were built. Four categories (partnership/sexuality, spirituality/religiousness, health perception, and overall health) were excluded following the qualitative content analysis because they were hardly or not at all mentioned by participants. Following on from the analysis of the expert discussion, one dimension was added (dignity), and two further categories were excluded (mobility and feeling of security in treatment). The resulting system consisted of six dimensions: emotional health, physical ailments, autonomy, social functionality, dignity, and resources.ConclusionThe identified dimensions of HrQoL in routine oncological care were found to differ from those used in existing HrQoL measurements for (cancer) patients. Further research is needed to test and evaluate the presented structure in a larger sample of cancer patients to further assess its psychometric properties.
Cross-Cultural Communication on Social Media: Review From the Perspective of Cultural Psychology and Neuroscience
Authors: Yuna Di , Xiaokun Liu , Jianing Li , Lu Han 
Published: March 2022

IntroductionIn recent years, with the popularity of many social media platforms worldwide, the role of “virtual social network platforms” in the field of cross-cultural communication has become increasingly important. Scholars in psychology and neuroscience, and cross-disciplines, are attracted to research on the motivation, mechanisms, and effects of communication on social media across cultures.Methods and AnalysisThis paper collects the co-citation of keywords in “cultural psychology,” “cross-culture communication,” “neuroscience,” and “social media” from the database of web of science and analyzes the hotspots of the literature in word cloud.ResultsBased on our inclusion criteria, 85 relevant studies were extracted from a database of 842 papers. There were 44 articles on cultural communication on social media, of which 26 were from the perspective of psychology and five from the perspective of neuroscience. There are 27 articles that focus on the integration of psychology and neuroscience, but only a few are related to cross-cultural communication on social media.ConclusionScholars have mainly studied the reasons and implications of cultural communication on social media from the perspectives of cultural psychology and neuroscience separately. Keywords “culture” and “social media” generate more links in the hot map, and a large number of keywords of cultural psychology and neuroscience also gather in the hot map, which reflects the trend of integration in academic research. While cultural characteristics have changed with the development of new media and virtual communities, more research is needed to integrate the disciplines of culture, psychology, and neuroscience.
Effectiveness of Vortioxetine in Patients With Major Depressive Disorder in Real-World Clinical Practice: Results of the RELIEVE Study
Authors: Mattingly Gregory W. , Ren Hongye , Christensen Michael Cronquist , Katzman Martin A. , Polosan Mircea , Simonsen Kenneth , Hammer-Helmich Lene 
Published: March 2022

BackgroundRandomized controlled clinical trials have shown vortioxetine to be efficacious and well tolerated for the treatment of major depressive disorder (MDD). The Real-Life Effectiveness of Vortioxetine in Depression (RELIEVE) study was undertaken to demonstrate the effectiveness and safety of vortioxetine for the treatment of MDD in routine clinical practice.MethodsRELIEVE was a 24-week, observational, prospective cohort study in outpatients with MDD initiating treatment with vortioxetine at their physician's discretion in routine care settings in Canada, France, Italy, and the USA (NCT03555136). The primary study outcome was patient functioning assessed by the Sheehan Disability Scale (SDS). Secondary outcomes included depression severity [9-item Patient Health Questionnaire (PHQ-9)], cognitive symptoms [5-item Perceived Deficits Questionnaire-Depression (PDQ-D-5)], and cognitive performance [Digit Symbol Substitution Test (DSST)]. Mixed models of repeated measures were used to assess change from baseline at week 24, adjusted for relevant confounders.ResultsA total of 737 patients were eligible for inclusion in the full analysis set. Most patients (73.7%) reported at least one comorbid medical condition, 56.0% had comorbid anxiety and 24.4% had comorbid generalized anxiety disorder. Improvement in least-squares (LS) mean SDS score from baseline to week 24 was 8.7 points. LS mean PHQ-9, PDQ-D-5 and DSST scores improved by 7.4, 4.6, and 6.2 points, respectively. Adverse events were observed in 21.2% of patients [most commonly, nausea (8.2% of patients)].ConclusionsThese results demonstrate the effectiveness and tolerability of vortioxetine for the treatment of MDD in a large and heterogeneous patient population representative of that encountered in routine clinical practice.
Multidrug-Resistant Methicillin-Resistant Coagulase-Negative Staphylococci in Healthy Poultry Slaughtered for Human Consumption
Authors: Silva Vanessa , Caniça Manuela , Ferreira Eugénia , Vieira-Pinto Madalena , Saraiva Cândido , Pereira José Eduardo , Capelo José Luis , Igrejas Gilberto , Poeta Patrícia 
Published: March 2022

Coagulase-negative staphylococci are commensals that are known to be prevalent in most environments, and they are also an important reservoir of antimicrobial-resistant genes. Staphylococcal infections in animal husbandry are a high economic burden. Thus, we aimed to determine the prevalence and species diversity of methicillin-resistant coagulase-negative staphylococci (MRCoNS) in poultry slaughtered for human consumption and to study the antimicrobial resistance of the isolates. Swab samples were recovered from 220 commercial chickens, homebred chickens and quails. Species identification was performed using MALDI-TOF. Antimicrobial susceptibility testing was performed by the disc diffusion method against 14 antimicrobials. The presence of antimicrobial-resistant genes was investigated by polymerase chain reaction. Totals of 11 (19.6%), 13 (20.3%), and 51 (51%) MRCoNS were isolated from commercial chickens, homebred chickens and quails, respectively. S. lentus was isolated from all homebred chickens, whereas 11 S. lentus and 2 S. urealyticus were isolated from commercial chickens. As for quails, the most prevalent MRCoNS were S. urealyticus. Almost all isolates had a multidrug-resistant profile and carried the mecA gene. Most isolates showed resistance to erythromycin, clindamycin, penicillin, tetracycline, ciprofloxacin and fusidic acid and harbored the ermA, ermB, ermC, mphC tetK, tetL, tetM and tetO genes. This study showed a frequent occurrence of multidrug resistance in MRCoNS isolated from healthy poultry in Portugal.
Exploring Peptaibol’s Profile, Antifungal, and Antitumor Activity of Emericellipsin A of Emericellopsis Species from Soda and Saline Soils
Authors: Kuvarina Anastasia E. , Gavryushina Irina A. , Sykonnikov Maxim A. , Efimenko Tatiana A. , Markelova Natalia N. , Bilanenko Elena N. , Bondarenko Sofiya A. , Kokaeva Lyudmila Y. , Timofeeva Alla V. , Serebryakova Marina V. , Barashkova Anna S. , Rogozhin Eugene A. , Georgieva Marina L. , Sadykova Vera S. 
Published: March 2022

Features of the biochemical adaptations of alkaliphilic fungi to exist in extreme environments could promote the production of active antibiotic compounds with the potential to control microorganisms, causing infections associated with health care. Thirty-eight alkaliphilic and alkalitolerant Emericellopsis strains (E. alkalina, E. cf. maritima, E. cf. terricola, Emericellopsis sp.) isolated from different saline soda soils and belonging to marine, terrestrial, and soda soil ecological clades were investigated for emericellipsin A (EmiA) biosynthesis, an antifungal peptaibol previously described for Emericellopsis alkalina. The analysis of the Emericellopsis sp. strains belonging to marine and terrestrial clades from chloride soils revealed another novel form with a mass of 1032.7 Da, defined by MALDI-TOF Ms/Ms spectrometers, as the EmiA lacked a hydroxyl (dEmiA). EmiA displayed strong inhibitory effects on cell proliferation and viability of HCT 116 cells in a dose- and time-dependent manners and induced apoptosis.
The Use of Metabolic Drug in the Treatment of Patients with Open-Angle Glaucoma
Authors: Пастух И.В. , Гончарова Н.А. , Недзвецкая О.В. , Чубенко И.О. 
Published: March 2022

Целью исследования явилось повышение эффективности лечения глаукомной оптической нейропатии (ГОН) путем применения метаболического препарата Кокарнит (фармацевтическая компания «World Medicine») в комплексном лечении пациентов с первичной открытоугольной глаукомой (ПОУГ). В исследование были включены 30 пациентов (41 глаз) с ПОУГ II–III (a) стадии, при компенсации внутриглазного давления (ВГД) инстилляциями капель бримонидина. Всем пациентам проводились стандартные офтальмологические, клинические и дополнительные инструментальные методы исследования. Для стимуляции зрительных функций пациентам назначались курсы поддерживающей терапии, включающие разные группы препаратов. В качестве метаболической терапии нами использовался препарат Кокарнит, который применялся внутримышечно по 3,0 мл ежедневно, на курс 9 инъекций.После проведенных курсов лечения у пациентов повышалась острота зрения, расширялись периферические границы поля зрения, уменьшались размеры центральных и парацентральных скотом, повышалась светочувствительность сетчатки. Наблюдаемый эффект сохранялся от 3 до 4 месяцев.
The aim of the investigation was to increase the effectiveness of the treatment of glaucoma optical neuropathy (GON) by using the metabolic drug Kokarnit (World Medicine pharmaceutical company) in the complex treatment of patients with primary open-angle glaucoma (POAG). The study included 30 patients (41 eyes) with II–III (a) stages of POAG, while compensating for intraocular pressure (IOP) with instillations of brimonidine drops. All patients underwent standard ophthalmological, clinical and additional instrumental studies. To stimulation maintain visual function, all patients underwent courses of therapy, including different groups of drugs. As a metabolic therapy, the drug Kokarnit was used, which was prescribed intramuscularly in 3.0 ml daily, for a course of 9 injections.After the courses of treatment, the patient’s visual acuity increased, the peripheral boundaries of the visual field expanded, and the sizes of central and paracentral scotomas decreased, raise photosensivity retina. The observed effect persisted for 3 to 4 months.
Molecular Biological Predictors of the Low Molecular Weight Sodium Hyaluronate Injections Effectiveness in Transplant Diseases Treatment
Authors: Семак Г.Р. , Жерко И.Ю. , Захарова В.А. , Летковская Т.А. , Клецкий С.К. 
Published: March 2022

Введение. Около 70% повторных кератопластик и кератопластик в исходе тяжелых хронических дистрофических заболеваний роговицы заканчиваются непрозрачным приживлением и развитием болезни трансплантата.Целью исследования стало определение молекулярных предикторов эффективности применения инъекционного 1%-ного натрия гиалуроната в лечении болезни трансплантата.Материалы и методы. В группе «Болезнь трансплантата» обследование и лечение проводилось 19 пациентам (20 глаз) в возрасте от 28 до 87 лет. Для изучения морфологических особенностей болезни трансплантата, проводимой терапии были использованы окрашивание гистологических препаратов гематоксилином и эозином; гистохимическое окрашивание (PAS-реакция, MSB, по Массону, конго красным; ИГХМ с использованием маркеров CD44, MMP9, TGFβ1.Результаты. Согласно полученным результатам, в 2/11 случаев отторжение трансплантата развивалось по типу клеточно-опосредованной цитотоксичности и в 4/11 случаев – по гуморальному типу. При этом в группе пациентов с признаками болезни трансплантата на фоне применения инъекционной формы гиалуроновой кислоты отмечается улучшение регенерационных свойств эпителия роговицы за счет увеличения эпителиальной экспрессии CD44 и снижение содержания биомолекулярных маркеров, ассоциированных с воспалением, что отражает уменьшение интенсивности экспрессии ММР-9 и TGFβ1 и их роли в последующей дезорганизации стромы роговицы.
Introduction. About 70% of repeated keratoplastics and keratoplastics in the outcome of severe chronic dystrophic corneal diseases result in opaque engraftment and the development of corneal transplant disease.The aim of the study was to determine molecular predictors of the injectable 1% sodium hyaluronate effectiveness in the treatment of transplant disease.Materials and methods. In the Transplant Disease group, 19 patients (20 eyes) aged 28 to 87 years were examined and treated. To study the morphological features of the transplant disease histological preparations stained with hematoxylin and eosin were made; histochemical staining (PAS reaction, MSB, according to Masson, Congo red); IHC using markers CD44, MMP9, TGFβ1 were performed.Results. According to the obtained results, in 2/11 cases cell-mediated transplant rejection developed, in 4/11 cases – antibodies-mediated. At the same time, in the group of patients treated with hyaluronic acid, an improvement in the regenerative properties of the corneal epithelium due to increase of epithelial expression of CD44 was shown. Decrease in the content of biomolecular markers associated with inflammation (MMP-9 and TGFβ1) was detected.
Dynamics of Indicators of Brain MRI at the Stages of Stroke Rehabilitation in the Vertebro-Basilar System
Authors: Бахадирова М.А. , Мирджураев Э.М. , Хусанходжаев Ж.У. , Бахадиров С.К. , Мирджураев Ж.Э. 
Published: March 2022

Цель. Изучить динамику показателей МРТ на этапах реабилитации инсульта в вертебробазилярной системе (ВБС) артерий головного мозга.Материалы и методы. МРТ проводили на аппарате Siemens с силой магнитного поля 1,5 тесла при поступлении в отделение, повторялось в пределах 7–10 суток до окончания 3-месячного срока после сосудистой катастрофы. Обследовано 126 пациентов с инсультом в ВБС. Результаты. Анализ статистической обработки клинико-неврологических показателей и данных МРТ пациентов с ишемическим инсультом в ВБС позволил выявить наличие достоверной (p<0,05) сильной корреляционной связи объема очага поражения и баллами по шкалеB. Lindmark в группе пациентов с инфарктом таламуса и затылочных долей (r=0,73 и r=0,68 соответственно); в группах пациентов с инфарктом мозжечка отмечена средняя корреляционная связь (r=0,49).Баллы по шкале B. Lindmark пациента, как правило, обратно пропорциональны объему очага поражения, но мы зафиксировали отсутствие корреляции объема очага поражения и баллов по шкале B. Lindmark у пациентов с инфарктом варолиева моста (r=0,0045), что свидетельствует об отсутствии связи размеров очага поражения и тяжести состояния пациентов.
Purpose. To study the dynamics of MRI indicators at the stages of stroke rehabilitation in the vertebrobasilar system of the cerebral arteries.Materials and methods. MRI was performed on the Siemens apparatus with the magnetic field strength of 1.5 Tesla upon admission to the department; it was repeated within 7–10 days until the end of the 3-month period after the vascular accident. We examined 126 patients with stroke in the VBS.Results. Analysis of the statistical processing of clinical and neurological parameters and MRI data of patients with IS in VBS revealed the presence of a significant (p<0.05) strong correlation between the volume of the lesion and scores on the B. Lindmark scale in the group of patients with thalamic infarction and occipital lobes (r=0.73 and r=0.68, respectively); the groups of patients with cerebellar infarction showed an average correlation (r=0.49).Patient’s B. Lindmark scores are usually inversely proportional to the lesion volume, but we recorded the lack of correlation between the lesion volume scores on the B. Lindmark scale in patients and pons infarction (r = 0.0045), which indicates that there was no correlation between the size of the lesion and the severity of the patient’s condition.
Complications of Surgical Treatment of the Oral and Oropharyngeal Cancer
Authors: Тризна Н.М. , Колядич Ж.В. 
Published: March 2022

Цель. Изучение осложнений хирургического лечения рака полости рта и ротоглотки.Материалы и методы. Изучены результаты хирургического лечения по числу и характеру послеоперационных осложнений у 164 пациентов со злокачественными опухолями полости рта и ротоглотки в зависимости от распространенности опухолевого процесса, объема хирургического вмешательства, метода реконструкции пострезекционного дефекта и предшествующего противоопухолевого лечения. Проанализированы послеоперационные хирургические осложнения по классификации Clavien − Dindo, причины возникновения послеоперационных осложнений после реконструктивных операций.Результаты. Частота развития послеоперационных осложнений составила 20,0% в группе пациентов, которым не проводилось одномоментное замещение пострезекционного дефекта, и 31,34% после реконструктивных операций (χ2=0,52; p=0,471). Показано, что степень тяжести местных послеоперационных осложнений после реконструктивных операций не зависела от предшествовавшей лучевой или химиолучевой терапии (r=0,158; p>0,05), а была обусловлена, различными патогенетическими механизмами и соответствующими клиническими проявлениями осложнений, распространенностью опухолевого процесса.Выводы. Проведенный анализ послеоперационных осложнений позволит совершенствовать алгоритм предоперационного и послеоперационного ведения пациентов со злокачественными опухолями полости рта и ротоглотки.
Purpose. To study surgical complications of the oral and oropharyngeal cancer.Materials and methods. The outcomes of surgical treatment of 164 patients with oral and oropharyngeal cancer were studied. The comparative analysis of postoperative complications was carried out depending on the prevalence of the tumor process, the volume of surgical intervention, the method of reconstruction of the post-resection defect, and previous treatment. Postoperativesurgical complications were analyzed according to the Clavien-Dindo classification; the causes of postoperative complications after reconstructive operations were studied.Results. The incidence of postoperative complications was 20.0% in the group of patients who did not undergo simultaneous post-resection defect replacement, and 31.34% - after reconstructive operations (χ2=0.52; p=0.471). It was showed that the severity of local postoperative complications did not depend on the previous radiotherapy or chemoradiotherapy (r=0.158; p>0.05), but it was due to various pathogenetic mechanisms and the corresponding clinical manifestations of complications, the prevalence of the tumor process.Conclusion. The analysis of surgical complications will help to improve the algorithm of preoperative and postoperative management of this category of patients with the oral and oropharyngeal cancer.