bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring Camelina sativa lipid metabolism regulation by combining gene co‐expression and DNA affinity purification analyses
Authors: Gomez‐Cano Fabio , Chu Yi‐Hsuan , Cruz‐Gomez Mariel , Abdullah Hesham M. , Lee Yun Sun , Schnell Danny J. , Grotewold Erich 
Published: January 2022

SUMMARYCamelina (Camelina sativa) is an annual oilseed plant that is gaining momentum as a biofuel cover crop. Understanding gene regulatory networks is essential to deciphering plant metabolic pathways, including lipid metabolism. Here, we take advantage of a growing collection of gene expression datasets to predict transcription factors (TFs) associated with the control of Camelina lipid metabolism. We identified approximately 350 TFs highly co‐expressed with lipid‐related genes (LRGs). These TFs are highly represented in the MYB, AP2/ERF, bZIP, and bHLH families, including a significant number of homologs of well‐known Arabidopsis lipid and seed developmental regulators. After prioritizing the top 22 TFs for further validation, we identified DNA‐binding sites and predicted target genes for 16 out of the 22 TFs tested using DNA affinity purification followed by sequencing (DAP‐seq). Enrichment analyses of targets supported the co‐expression prediction for most TF candidates, and the comparison to Arabidopsis revealed some common themes, but also aspects unique to Camelina. Within the top potential lipid regulators, we identified CsaMYB1, CsaABI3AVP1‐2, CsaHB1, CsaNAC2, CsaMYB3, and CsaNAC1 as likely involved in the control of seed fatty acid elongation and CsaABI3AVP1‐2 and CsabZIP1 as potential regulators of the synthesis and degradation of triacylglycerols (TAGs), respectively. Altogether, the integration of co‐expression data and DNA‐binding assays permitted us to generate a high‐confidence and short list of Camelina TFs involved in the control of lipid metabolism during seed development.
Pharmacokinetics and pharmacodynamics studies of a loading dose of cisatracurium in critically ill patients with respiratory failure
Authors: Panusitthikorn Panadda , Suthisisang Chuthamanee , Tangsujaritvijit Viratch , Nosoongnoen Wichit , Dilokpattanamongkol Pitchaya 
Published: January 2022

Abstract
Background
Previous studies reported a slow neuromuscular response with the currently recommended dose of cisatracurium in critically ill patients. Pharmacokinetic and pharmacodynamic studies of cisatracurium in critically ill patients are still limited. To our knowledge, this is the first study performed to better understand the pharmacokinetics (PKs) and pharmacodynamics (PDs) of a loading dose of cisatracurium and to identify factors that affect PK and PD changes in critically ill patients.

Methods
A prospective PKs and PDs study was designed. Arterial blood samples of 10 critically ill patients with respiratory failure were collected after administering a loading dose of 0.2 mg/kg of cisatracurium. Plasma cisatracurium and laudanosine concentrations were determined using liquid chromatography-tandem mass spectrometry. The achievement of the desired pharmacodynamic response was evaluated by both 1) clinical assessment and 2) train-of-four monitoring. The PK/PD indices were analyzed for their correlation with patient’characteristics and other factors.

Results
The one-compartment model best described the plasma pharmacokinetic parameters of cisatracurium. The volume of distribution at steady state and total clearance were 0.11 ± 0.04 L/kg and 2.74 ± 0.87 ml/minute/kg, respectively. The mean time to train-of-four 0/4 was 6 ± 3.86 minutes. A time to the desired pharmacodynamic response of less than 5 minutes was found in 10% of the patients. A positive correlation was found between cisatracurium concentration and albumin levels and between pharmacokinetics data and patient factors [partial pressure of carbon dioxide and respiratory alkalosis].

Conclusion
The currently recommended loading dose of cisatracurium might not lead to the desired pharmacodynamic response in critically ill patients with respiratory failure.

Trial registration
ClinicalTrials.gov, NCT03337373. Registered on 9 November 2017
Electrocardiographic diagnosis of acute myocardial infarction in a pacemaker patient: a case report
Authors: Du Xing , Zhang Yongjun 
Published: January 2022

Abstract
Background
The electrocardiographic diagnosis of acute myocardial infarction (AMI) in the setting of cardiac pacing is often challenging. The original Sgarbossa criteria proposed in 1996 were demonstrated to be valid for diagnosis of AMI in both ventricular paced rhythm and left bundle branch block. To improve accuracy, the modified Sgarbossa criteria (MSC) were proposed.

Case presentation
We presented a case of electrocardiographic diagnosis of AMI in a pacemaker patient. The Electrocardiogram (ECG) was false negative by using the original Sgarbossa criteria, whereas true positive by the MSC at a ratio of − 0.20.

Conclusions
The application of MSC using an appropriate ratio (− 0.20 or − 0.25) may facilitate a timely diagnosis of AMI. Physicians should carefully choose the appropriate cutoff in a case-by-case basis.
The effect of intra-vaginal oxytocin on sexual function in breastfeeding mothers: a randomized triple-blind placebo-controlled trial
Authors: Mesbahi Arezu , Mohammad-Alizadeh-Charandabi Sakineh , Ghorbani Zahra , Mirghafourvand Mojgan 
Published: January 2022

Abstract
Background
Considering the importance of sexual function, high prevalence of sexual dysfunction (especially dyspareunia caused by atrophic vaginitis) in breastfeeding women, and lack of effective interventions, the present research aimed to determine the effect of oxytocin (OXT) vaginal gel on sexual function (primary outcome), sexual satisfaction, and depression (secondary outcomes) in the breastfeeding women.

Methods
This randomized triple-blind controlled trial was conducted on 64 breastfeeding women who referred to health centers in the city of Tabriz, Iran, in 2020-21. Participants were equally assigned to intervention/control groups using block randomization. 200 IU of OXT vaginal gel was given to the participants in the intervention group daily for eight week and the same protocol was carried out for the control group with placebo. Standard questionnaires of Female Sexual Function Index (FSFI), Edinburgh Postpartum Depression Scale (EPDS) and Sexual satisfaction scale for women (SSSW) were completed at baseline and 8 weeks after intervention. ANCOVA test was used to compare post-intervention mean score of the groups, adjusted for the baseline values.

Results
After intervention, there was no statistically significant difference between groups in terms of mean total score of FSFI (Adjusted Mean Difference (AMD): 1.14; 95% Confidence Interval (95% CI): -1.28 to 9.16; P= 0.349) and sexual satisfaction (AMD: 5.01; 95% CI: -0.53 to 10.56; P= 0.075). However, there was statistically significant difference between the groups in terms of mean scores of sexual contentment (AMD: 1.56; 95% CI: 0.29 to 2.83; P = 0.017) and depression (AMD: -1.90; 95% CI: -1.27 to -2.54; P < 0.001). One participant in the OXT group and one participant in the placebo group reported mild uterine contraction and one person in the placebo group reported vaginal burning sensations.

Conclusions
No evidence was found for the effects of OXT gel in the improvement of FSFI, even though, OXT significantly improved sexual satisfaction in the domain of contentment, and improved the symptoms of depression in comparison to the placebo group. However, a definite conclusion requires more research in this regard.

Trial registration
the Iranian Registry of Clinical Trials (IRCT), code: IRCT20120718010324N55, Date of registration: 27/05/2020, URL: https://en.irct.ir/user/trial/44986/view.
Recurrent graft failure secondary to portal vein steal syndrome: a case report with an unusual indication for a third liver transplant
Authors: Tropea Alessandro , Barbàra Marco , Pagano Duilio , Marrone Gianluca , Petridis Ioannis , Li Petri Sergio , Cintorino Davide , Gruttadauria Salvatore 
Published: January 2022

Abstract
Background
Portal vein shunt is common in chronic hepatic diseases and after a liver transplant. Ensuring a satisfactory portal flow is essential to support a rapid liver recovery, of paramount importance to meet the recipient’s metabolic needs.

Case presentation
We report the case of a 32-year-old female undergoing a third liver transplant due to recurrence of graft failure secondary to portosystemic shunting. The patient, affected with biliary atresia, was first transplanted in 2009 with a right split liver graft. The clinical course was complicated by biliary stenosis of the Roux-en-Y anastomosis and multiple episodes of acute rejection treated with steroid boluses, plastic dilation of the biliary anastomosis, and biliary catheter placement. Unfortunately, in 2017 a liver biopsy showed an autoimmunity with histological evidence of ANA 1:80 (granular and nucleolar pattern). This was a contributing factor of liver function impairment, leading to the need to perform a second liver transplant, complicated by an acute rejection, with only a partial response to steroid therapy. Due to the further worsening of the liver function (MELD: 40, Child–Pugh: C11), the patient was relisted for a liver transplant. After five days, she received her third liver transplant, with an entire graft of an AB0 identical group. Intraoperative exploration revealed multiple collaterals and large splenocaval shunts, with a significant alteration of the portal flow and hypertension, isolated and closed with a vascular stapler to restore the graft's regular portal vein flow.

Conclusions
In patients listed for a liver transplant, portal steal syndrome should be identified prior to the transplant. Our recommendation is to consider intraoperative or perioperative closure of the portal collateral varices.
Survival outcome of local vs. radical excision in rectal gastrointestinal stromal tumor: a SEER database analysis
Authors: Wei Jianchang , Zhong Junbin , Chen Zhuanpeng , Huang Qing , Wei Fang , Wang Qiang , Cao Jie 
Published: January 2022

Abstract
Background
The choice of surgical strategy for patients with rectal gastrointestinal stromal tumor (GIST) remains controversial. This study aims to address whether the surgical procedure [local excision (LE) vs. radical excision (RE)] influences the survival outcomes.

Methods
The information of the patients recruited in this study was obtained from the Surveillance, Epidemiology, and End Results (SEER) database. A survival curve was used to evaluate the differences in cancer-specific survival (CSS).

Results
No significant difference was detected in the CSS between the LE and RE groups. Also, no significant differences were observed in the CSS between the two groups with respect to different T classification, N classification, tumor differentiation, tumor size, regional LN surgery, age, gender, race, chemotherapy, and radiotherapy. The T classification and age were independent prognostic factors in rectal GIST patients.

Conclusions
LE and RE have similar survival time after surgery, and LE could be considered as an effective surgical approach for rectal GIST.
Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses
Authors: Murata Daiki , Ishikawa Shingo , Sunaga Takafumi , Saito Yasuo , Sogawa Takeshi , Nakayama Koichi , Hobo Seiji , Hatazoe Takashi 
Published: January 2022

Abstract
Background
Medical interventions for subchondral bone cysts in horses have been extensively studied. This study investigated the regeneration of articular cartilage and subchondral bone with scaffold-free three-dimensional (3D) constructs of equine synovial membrane-derived mesenchymal stem cells (SM-MSCs) isolated from three ponies and expanded until over 1.0 × 107 cells at passage 2 (P2).

Results
SM-MSCs were strongly positive for CD11a/CD18, CD44, and major histocompatibility complex (MHC) class I; moderately positive for CD90, CD105, and MHC class II; and negative for CD34 and CD45 on flow cytometry and differentiated into osteogenic, chondrogenic, and adipogenic lineages in the tri-lineage differentiation assay. After culturing SM-MSCs until P3, we prepared a construct (diameter, 6.3 mm; height, 5.0 mm) comprising approximately 1920 spheroids containing 3.0 × 104 cells each. This construct was confirmed to be positive for type I collagen and negative for type II collagen, Alcian blue, and Safranin-O upon histological analysis and was subsequently implanted into an osteochondral defect (diameter, 6.8 mm; depth, 5.0 mm) at the right femoral medial condyle. The contralateral (left femoral) defect served as the control. At 3 and 6 months after surgery, the radiolucent volume (RV, mm3) of the defects was calculated based on multiplanar reconstruction of computed tomography (CT) images. Magnetic resonance (MR) images were evaluated using a modified two-dimensional MR observation of cartilage repair tissue (MOCART) grading system, while macroscopic (gross) and microscopic histological characteristics were scored according to the International Cartilage Repair Society (ICRS) scale. Compared to the control sites, the implanted defects showed lower RV percentages, better total MOCART scores, higher average gross scores, and higher average histological scores.

Conclusions
Implantation of a scaffold-free 3D-construct of SM-MSCs into an osteochondral defect could regenerate the original structure of the cartilage and subchondral bone over 6 months post-surgery in horses, indicating the potential of this technique in treating equine subchondral bone cysts.
Narcissism and suicide risk
Authors: Gabbard Glen O. 
Published: January 2022

AbstractWe all have narcissism, but in some cases, the perception of narcissism becomes extreme and pathological. Systematic research has shown that there are three subtypes typical of narcissistic personality disorder: the grandiose/oblivious, the vulnerable/hypervigilant, and the high-functioning subtype. Both biological and psychological factors are at work, but the true cause of pathological narcissism has not been established. The psychotherapy of narcissistic personality disorder (NPD) is complicated and often frustrating because of the difficulty in engaging a person with narcissistic personality disorder in a psychotherapeutic process. Suicide risk is not rare in patients with narcissism, particularly in the context of severe narcissistic injury, where the patient feels shamed and/or vilified. In conclusion, narcissistic patients are difficult to treat, but the risk of suicide makes it imperative for clinicians to stay involved in the treatment and assist the patient in understanding their vulnerabilities.
“The medications are the decision-makers…” Making reproductive and medication use decisions among female patients with rheumatoid arthritis: a constructivist grounded theory
Authors: Rebić Nevena , Munro Sarah , Garg Ria , Hazlewood Glen , Amiri Neda , Baldwin Corisande , Ensworth Stephanie , Proulx Laurie , De Vera Mary A. 
Published: January 2022

AbstractObjectiveTo examine how female patients with RA form decisions about having children, pregnancy, and medication use.MethodsWe employed a constructivist grounded theory design and recruited female participants who are 18 years or older, have a rheumatologist-confirmed RA diagnosis, live in Canada, and are able to communicate in English or French. We collected data through semi-structured individual and focus group interviews using telephone or video conferencing technology. Data collection and analysis were iterative, employed theoretical sampling, reflexive journaling, and peer debriefing, and culminated in a theoretical model.ResultsWe recruited 21 participants with a mean age of 34 years and median 10 years since RA diagnosis. Overall, 33% had never been pregnant, 57% had previously been pregnant, and 10% were pregnant at the time of interview. Of those who had experienced pregnancy, 64% had at least one pregnancy while diagnosed with RA and of those, 56% used DMARD(s) during a pregnancy. We constructed a patient-centred framework depicting the dynamic relationships between 4 decision-making processes—(1) using medications, (2) having children, (3) planning pregnancy, and (4) parenting—and the substantial impact of healthcare providers on patients’ experiences making these decisions. These processes were further influenced by participants’ intersecting identities and contextual factors, particularly attitudes towards health and medications, disease onset and severity, familial support system, and experiences interacting with the healthcare system.ConclusionOur framework provides insight into how patients make reproductive decisions in the context of managing RA and the opportunities for providers to support them at each decision-making process. A patient-centred care approach is suggested to support female patients with RA in making reproductive and medication choices aligning with their individual desires, needs, and values.
Novel γ-sarcoglycan interactors in murine muscle membranes
Authors: Smith Tara C. , Vasilakos Georgios , Shaffer Scott A. , Puglise Jason M. , Chou Chih-Hsuan , Barton Elisabeth R. , Luna Elizabeth J. 
Published: January 2022

AbstractBackgroundThe sarcoglycan complex (SC) is part of a network that links the striated muscle cytoskeleton to the basal lamina across the sarcolemma. The SC coordinates changes in phosphorylation and Ca++-flux during mechanical deformation, and these processes are disrupted with loss-of-function mutations in gamma-sarcoglycan (Sgcg) that cause Limb girdle muscular dystrophy 2C/R5.MethodsTo gain insight into how the SC mediates mechano-signaling in muscle, we utilized LC-MS/MS proteomics of SC-associated proteins in immunoprecipitates from enriched sarcolemmal fractions. Criteria for inclusion were co-immunoprecipitation with anti-Sgcg from C57BL/6 control muscle and under-representation in parallel experiments with Sgcg-null muscle and with non-specific IgG. Validation of interaction was performed in co-expression experiments in human RH30 rhabdomyosarcoma cells.ResultsWe identified 19 candidates as direct or indirect interactors for Sgcg, including the other 3 SC proteins. Novel potential interactors included protein-phosphatase-1-catalytic-subunit-beta (Ppp1cb, PP1b) and Na+-K+-Cl−-co-transporter NKCC1 (SLC12A2). NKCC1 co-localized with Sgcg after co-expression in human RH30 rhabdomyosarcoma cells, and its cytosolic domains depleted Sgcg from cell lysates upon immunoprecipitation and co-localized with Sgcg after detergent permeabilization. NKCC1 localized in proximity to the dystrophin complex at costameres in vivo. Bumetanide inhibition of NKCC1 cotransporter activity in isolated muscles reduced SC-dependent, strain-induced increases in phosphorylation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). In silico analysis suggests that candidate SC interactors may cross-talk with survival signaling pathways, including p53, estrogen receptor, and TRIM25.ConclusionsResults support that NKCC1 is a new SC-associated signaling protein. Moreover, the identities of other candidate SC interactors suggest ways by which the SC and NKCC1, along with other Sgcg interactors such as the membrane-cytoskeleton linker archvillin, may regulate kinase- and Ca++-mediated survival signaling in skeletal muscle.
Antioxidant and antibacterial screening of honey of Hiterotrogona itama collected from differents meliponiculture areas in East Kalimantan, Indonesia
Authors: SAPUTRA SUROTO HADI , SARAGIH BERNATAL , KUSUMA IRAWAN WIJAYA , ARUNG ENOS TANGKE 
Published: January 2022

Abstract. Saputra SH, Saragih B, Kusuma IW, Arung ET. 2021. Antioxidant and antibacterial screening of honey of Hiterotrogona itama collected from differents meliponiculture areas in East Kalimantan, Indonesia. Nusantara Bioscience 13: 232-237. Heterotrigona itama (Cockerell, 1918) is a stingless bee that exits in several areas in East Kalimantan of Indonesia. This study aimed to analyze the phytochemicals, antioxidant activity of honey of this stingless bee from several regions in East Kalimantan. Phytochemical testing was carried out qualitatively, while antioxidant activity was performed using DPPH (1.1-diphenyl-2-picrylhydrazyl-radical) radical scavenging assay. The antimicrobial activity of Staphylococcus aureus and Escherichia coli was performed through in vitro test. The results showed that the honey of stingless H. itama bee collected from five cultivated areas in East Kalimantan contained flavonoid, coumarin, steroids, carotenoids, and some of which had alkaloid and tannin compounds. The antioxidant activity (IC50) was observed between 43,54 ppm-71,27 ppm, while the strong antimicrobial activity of this honey was found against S. aureus and E. coli.
Proyecto de vida en estudiantes de medicina de una universidad pública
Authors: Izaguirre Sotomayor Manuel 
Published: January 2022

Introducción. El proyecto de vida como instrumento pedagógico ayudará al estudiante de medicina a elaborar una visión de futuro que garantice su permanencia en la universidad, al asumir que la profesión no es el fin, sino el medio para hacer realidad su sueño de un futuro mejor para él, su familia y la sociedad, con resultados académicos satisfactorios, menores índices de desaprobación y deserción, al mejorar su capacidad de tomar decisiones. Objetivos. Conocer el proyecto de vida, sueños, visiones y metas de los estudiantes del sexto año de la Facultad de Medicina de la Universidad Nacional Mayor de San Marcos. Métodos. Estudio observacional, descriptivo transversal, de enfoque cuantitativo, utilizando como instrumento un cuestionario de preguntas abiertas. Resultados. El 94% manifestaron tener proyecto de vida, 98% tener sueños, 96% tener visión y 76% tener metas a corto y largo plazo. El 52% tuvieron como sueño culminar la carrera, la residencia y la especialidad con éxito, ejercer la profesión, tener familia y viajar. El 86% manifestaron tener por visión ser buen profesional, buen especialista, empresario, trabajar en el estado, ser docente, investigador, tener familia, viajar, tener casa propia y ser feliz. Conclusiones. En los estudiantes de medicina del sexto año de la Facultad de Medicina UNMSM, el 94% manifestaron tener proyecto de vida, 98% tener sueños, 96% visión, y 76% tener metas a corto y largo plazo.
Theoretical Biosensor Design for Gold- PVA Surface Plasmon Resonance Layers
Authors: Kadhum Farah Jawad , Saeed Asrar Abdulmunem , AL-kadhemy Mahasin F. Hadi , Al-Zuky Ali A. Dawood , Al- Saleh Anwar H. 
Published: January 2022

. Surface Plasmon Resonance (SPR) technology has been adapted to produce a sensitive optical sensor and Biosensor applications. Simulation analysis ( in Matlab) has been made for SPR for gold (Au) layer with thickness (40 nm) and Polyvinyl Alcohol (PVA) polymer with various thickness (10, 20, 30, 40, 50, 60, 70 and 80 nm) deposited on glass prism type D-ZLAF50_Dense lanthanum flint. The sensitive layer was air (n=1). The analysis was taken for different wavelengths from Ultra-Violet wavelength 100 nm to Near Infra- Red wavelength 1000 nm. The properties of Î¸SPR have been calculated from plotted reflectance against incident angle Î¸incid.. The SPR sensitivity (S) was calculated. The results give efficient detection in change of sensitive layer refractive index (0, 0.04, 0.08 and 0.12), and obtained the maximum sensitivity 207.5 for thickness of PVA 40 nm and 50 nm, âˆ†n= 0.08 at wavelength 800nm and 1000nm, respectively. The best sensitivity in visible region (700 nm) is 158.33 for thickness 20nm, âˆ†n= 0.12.