bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence of Seasonal Uplift in the Argentière Glacier (Mont Blanc Area, France)
Authors: Vincent Christian , Gilbert Adrien , Walpersdorf Andrea , Gimbert Florent , Gagliardini Olivier , Jourdain Bruno , Roldan Blasco Juan Pedro , Laarman Olivier , Piard Luc , Six Delphine , Moreau Luc , Cusicanqui Diego , Thibert Emmanuel 
Published: July 2022

AbstractThe hydromechanical processes by which basal water controls sliding at the glacier bed are poorly known, despite glacier basal motion being responsible for a large part of ice flux in temperate alpine glaciers. Previous studies suggest that sliding strongly relates to the quantity of water being stored at the ice‐bedrock interface. However, this water storage is difficult to quantify accurately on the basis of surface‐motion observations, given that uplift can also be affected by changes in vertical‐strain rates and sliding velocity change. Here, we use a comprehensive data set of in situ measurements performed over 2 years on the Argentière Glacier in the French Alps to investigate the relationships between horizontal and vertical velocities, basal sliding, subglacial runoff and bed separation. We observe strikingly large uplifts varying spatially between 0.20 and 0.90 m over the winter/spring seasons between January and June and with a consistent spatial pattern from 1 year to another. We show, based on observations and three dimensional ice‐flow modeling, that these large uplifts cannot be explained solely by changes in strain rates or in sliding up an inclined bed. Our results reveal that more than 80% of the observed uplift is related to enhanced bed separation through cavitation, allowing us to estimate the volume occupied by water‐filled subglacial cavities. Our interpretation of uplift being mainly caused by increased cavitation is also consistent with an associated increase in the observed surface horizontal velocity. These findings provide important observational constraints for testing subglacial hydrological models.
Post‐Rifting Relaxation During 2015–2020 Following the Bárðarbunga‐Holuhraun Dike Intrusion and Eruption in Iceland
Authors: Li Siqi , Grapenthin Ronni , Sigmundsson Freysteinn , Drouin Vincent , Hreinsdóttir Sigrún , Ófeigsson Benedikt G. 
Published: July 2022

AbstractPost‐rifting ground deformation may be driven by viscoelastic relaxation of stresses generated by dike intrusions. The single‐dike intrusion of the 2014–2015 Bárðarbunga eruption in Iceland presents an opportunity for a detailed study of this process. We use continuous Global Navigation Satellite System (GNSS) and Interferometric Synthetic Aperture Radar (InSAR) velocity fields to analyze the 2015–2020 post‐rifting deformation, showing uplift on both sides of the dike and horizontal displacement away from the dike after correcting for background deformation. Two GNSS stations experience baseline lengthening at a rate of 19 mm/yr in the direction perpendicular to the strike of the dike. A two‐layer viscoelastic model with a 0.4 × 1019 Pa s viscoelastic half‐space overlain by an 18 km thick elastic layer best explains the observed horizontal and vertical InSAR and GNSS displacements. The model misfit space shows a second regime of good fit, likely driven by deformation near the dike that may result from cooling compaction of the emplaced dike.
Sex-specific changes in metabolism during the transition from chow to high-fat diet feeding are abolished in response to dieting in C57BL/6J mice
Authors: Oraha Jennifer , Enriquez Ronaldo F. , Herzog Herbert , Lee Nicola J. 
Published: July 2022

Abstract
Background/Objective
Female mice are often excluded from diet-induced obesity studies as they are more resistant to the obesifying effects of a high-fat diet (HFD). However, the underlying mechanisms behind this sex disparity may actually have important implications for the development and management of obesity in humans. Therefore, we systematically investigated the immediate sex-specific effects of transitioning to a HFD in C57BL/6J mice as well as monitored whether these effects are altered after sustained HFD feeding and whether sex affects the response to a return to chow, representative of dieting.

Methods
Dual X-ray absorptiometry (DXA) analysis of body composition, indirect calorimetry measurements, and qPCR analysis of hypothalamic and brainstem regions were performed on male and female C57BL/6J mice.

Results
HFD had immediate and dramatic effects in males, increasing fat mass by 58% in the first 3 days. The resistance to the obesifying effect of HFD in females was linked both to an ability to maintain activity levels as well as to an immediate and significantly enhanced reduction in respiratory quotient (RQ), suggesting a greater ability to utilise fat in the diet as a source of fuel. Mechanistically, this sex disparity may be at least partially due to inherent sex differences in the catabolic (POMC/CART) versus anabolic (NPY/AgRP) neurological signalling pathways. Interestingly, the reintroduction of chow following HFD had immediate and consistent responses between the sexes with body composition and most metabolic parameters normalised within 3 days. However, both sexes displayed elevated hypothalamic Npy levels reminiscent of starvation. The difference in RQ seen between the sexes on HFD was immediately abolished suggesting similar abilities to burn fat reserves for fuel.

Conclusions
C57BL/6J mice have markedly different sex-specific behavioural and metabolic responses to the introduction as well as the sustained intake of a HFD, but consistent responses to a dieting situation.
Signaling pathways involved in ischemic stroke: molecular mechanisms and therapeutic interventions
Authors: Qin Chuan , Yang Sheng , Chu Yun-Hui , Zhang Hang , Pang Xiao-Wei , Chen Lian , Zhou Luo-Qi , Chen Man , Tian Dai-Shi , Wang Wei 
Published: July 2022

AbstractIschemic stroke is caused primarily by an interruption in cerebral blood flow, which induces severe neural injuries, and is one of the leading causes of death and disability worldwide. Thus, it is of great necessity to further detailly elucidate the mechanisms of ischemic stroke and find out new therapies against the disease. In recent years, efforts have been made to understand the pathophysiology of ischemic stroke, including cellular excitotoxicity, oxidative stress, cell death processes, and neuroinflammation. In the meantime, a plethora of signaling pathways, either detrimental or neuroprotective, are also highly involved in the forementioned pathophysiology. These pathways are closely intertwined and form a complex signaling network. Also, these signaling pathways reveal therapeutic potential, as targeting these signaling pathways could possibly serve as therapeutic approaches against ischemic stroke. In this review, we describe the signaling pathways involved in ischemic stroke and categorize them based on the pathophysiological processes they participate in. Therapeutic approaches targeting these signaling pathways, which are associated with the pathophysiology mentioned above, are also discussed. Meanwhile, clinical trials regarding ischemic stroke, which potentially target the pathophysiology and the signaling pathways involved, are summarized in details. Conclusively, this review elucidated potential molecular mechanisms and related signaling pathways underlying ischemic stroke, and summarize the therapeutic approaches targeted various pathophysiology, with particular reference to clinical trials and future prospects for treating ischemic stroke.
Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia
Authors: Grüninger Patricia K. , Uhl Franziska , Herzog Heike , Gentile Gaia , Andrade-Martinez Marta , Schmidt Tobias , Han Kyuho , Morgens David W. , Bassik Michael C. , Cleary Michael L. , Gorka Oliver , Zeiser Robert , Groß Olaf , Duque-Afonso Jesús 
Published: July 2022

AbstractB-cell precursor acute lymphoblastic leukemias (B-ALL) are characterized by the activation of signaling pathways, which are involved in survival and proliferation of leukemia cells. Using an unbiased shRNA library screen enriched for targeting signaling pathways, we identified MTOR as the key gene on which human B-ALL E2A-PBX1+ RCH-ACV cells are dependent. Using genetic and pharmacologic approaches, we investigated whether B-ALL cells depend on MTOR upstream signaling pathways including PI3K/AKT and the complexes MTORC1 or MTORC2 for proliferation and survival in vitro and in vivo. Notably, the combined inhibition of MTOR and AKT shows a synergistic effect on decreased cell proliferation in B-ALL with different karyotypes. Hence, B-ALL cells were more dependent on MTORC2 rather than MTORC1 complex in genetic assays. Using cell metabolomics, we identified changes in mitochondrial fuel oxidation after shRNA-mediated knockdown or pharmacological inhibition of MTOR. Dependence of the cells on fatty acid metabolism for their energy production was increased upon inhibition of MTOR and associated upstream signaling pathways, disclosing a possible target for a combination therapy. In conclusion, B-ALL are dependent on the PI3K/AKT/MTOR signaling pathway and the combination of specific small molecules targeting this pathway appears to be promising for the treatment of B-ALL patients.
A novel mechanism for macrophage pyroptosis in rheumatoid arthritis induced by Pol β deficiency
Authors: Gu Lili , Sun Yuling , Wu Ting , Chen Ge , Tang Xiaojun , Zhao Lianfeng , He Lingfeng , Hu Zhigang , Sun Lingyun , Pan Feiyan , Yin Zhimin , Guo Zhigang 
Published: July 2022

AbstractRheumatoid arthritis (RA) is a chronic and inflammatory autoimmune disease. Macrophage pyroptosis, a proinflammatory form of cell death, is critically important in RA; however, the detailed mechanism underlying pyroptosis induction is not yet well understood. Here, we report that DNA polymerase β (Pol β), a key enzyme in base excision repair, plays a pivotal role in RA pathogenesis. Our data shows that Pol β expression is significantly decreased in peripheral blood mononuclear cells (PBMCs) from active RA patients and collagen-induced arthritis (CIA) mice, and Pol β deficiency increases the incidence of RA, macrophage infiltration, and bone destruction in CIA mouse models. In vitro, experiments showed that Pol β deficiency exacerbated macrophage pyroptosis induced by LPS plus ATP, while overexpression of Pol β inhibited macrophage pyroptosis. Further characterization revealed that Pol β knockout resulted in DNA damage accumulation and cytosolic dsDNA leakage, which activated the cGAS-STING-NF-κB signaling pathway and upregulated the expression of NLRP3, IL-1 β, and IL-18. In conclusion, our findings clarify the influence of Pol β on the development of RA and provide a detailed explanation for the STING-NF-κB pathway to induce macrophage pyroptosis.
The inequality labor loss risk from future urban warming and adaptation strategies
Authors: He Cheng , Zhang Yuqiang , Schneider Alexandra , Chen Renjie , Zhang Yan , Ma Weichun , Kinney Patrick L. , Kan Haidong 
Published: July 2022

AbstractHeat-induced labor loss is a major economic cost related to climate change. Here, we use hourly heat stress data modeled with a regional climate model to investigate the heat-induced labor loss in 231 Chinese cities. Results indicate that future urban heat stress is projected to cause an increase in labor losses exceeding 0.20% of the total account gross domestic product (GDP) per year by the 2050s relative to the 2010s. In this process, certain lower-paid sectors could be disproportionately impacted. The implementation of various urban adaptation strategies could offset 10% of the additional economic loss per year and help reduce the inequality-related impact on lower-paid sectors. So future urban warming can not only damage cities as a whole but can also contribute to income inequality. The implication of adaptation strategies should be considered in regard to not only cooling requirements but also environmental justice.
Clinical relevance of molecular characteristics in Burkitt lymphoma differs according to age
Authors: Burkhardt Birgit , Michgehl Ulf , Rohde Jonas , Erdmann Tabea , Berning Philipp , Reutter Katrin , Rohde Marius , Borkhardt Arndt , Burmeister Thomas , Dave Sandeep , Tzankov Alexandar , Dugas Martin , Sandmann Sarah , Fend Falko , Finger Jasmin , Mueller Stephanie , Gökbuget Nicola , Haferlach Torsten , Kern Wolfgang , Hartmann Wolfgang , Klapper Wolfram , Oschlies Ilske , Richter Julia , Kontny Udo , Lutz Mathias , Maecker-Kolhoff Britta , Ott German , Rosenwald Andreas , Siebert Reiner , von Stackelberg Arend , Strahm Brigitte , Woessmann Wilhelm , Zimmermann Martin , Zapukhlyak Myroslav , Grau Michael , Lenz Georg 
Published: July 2022

AbstractWhile survival has improved for Burkitt lymphoma patients, potential differences in outcome between pediatric and adult patients remain unclear. In both age groups, survival remains poor at relapse. Therefore, we conducted a comparative study in a large pediatric cohort, including 191 cases and 97 samples from adults. WhileTP53andCCND3mutation frequencies are not age related, samples from pediatric patients showed a higher frequency of mutations inID3,DDX3X, ARID1AandSMARCA4, while several genes such asBCL2andYY1AP1are almost exclusively mutated in adult patients. An unbiased analysis reveals a transition of the mutational profile between 25 and 40 years of age. Survival analysis in the pediatric cohort confirms thatTP53mutations are significantly associated with higher incidence of relapse (25 ± 4% versus 6 ± 2%, p-value 0.0002). This identifies a promising molecular marker for relapse incidence in pediatric BL which will be used in future clinical trials.
Programmable synthetic cell networks regulated by tuneable reaction rates
Authors: Zambrano Adrian , Fracasso Giorgio , Gao Mengfei , Ugrinic Martina , Wang Dishi , Appelhans Dietmar , deMello Andrew , Tang T-Y. Dora 
Published: July 2022

AbstractCoupled compartmentalised information processing and communication via molecular diffusion underpin network based population dynamics as observed in biological systems. Understanding how both compartmentalisation and communication can regulate information processes is key to rational design and control of compartmentalised reaction networks. Here, we integrate PEN DNA reactions into semi-permeable proteinosomes and characterise the effect of compartmentalisation on autocatalytic PEN DNA reactions. We observe unique behaviours in the compartmentalised systems which are not accessible under bulk conditions; for example, rates of reaction increase by an order of magnitude and reaction kinetics are more readily tuneable by enzyme concentrations in proteinosomes compared to buffer solution. We exploit these properties to regulate the reaction kinetics in two node compartmentalised reaction networks comprised of linear and autocatalytic reactions which we establish by bottom-up synthetic biology approaches.
Effect of spin-orbit coupling on the high harmonics from the topological Dirac semimetal Na3Bi
Authors: Tancogne-Dejean Nicolas , Eich Florian G. , Rubio Angel 
Published: July 2022

AbstractIn this work, we performed extensive first-principles simulations of high-harmonic generation in the topological Diract semimetal Na3Bi using a first-principles time-dependent density functional theory framework, focusing on the effect of spin-orbit coupling (SOC) on the harmonic response. We also derived an analytical model describing the microscopic mechanism of strong-field dynamics in presence of spin-orbit coupling, starting from a locally U(1) × SU(2) gauge-invariant Hamiltonian. Our results reveal that SOC: (i) affects the strong-field excitation of carriers to the conduction bands by modifying the bandstructure of Na3Bi, (ii) makes each spin channel reacts differently to the driven laser by modifying the electron velocity (iii) changes the emission timing of the emitted harmonics. Moreover, we show that the SOC affects the harmonic emission by directly coupling the charge current to the spin currents, paving the way to the high-harmonic spectroscopy of spin currents in solids.
The Timed Up and Go test predicts frailty in patients with COPD
Authors: Albarrati Ali M. , Gale Nichola S. , Munnery Margaret M. , Reid Natasha , Cockcroft John R. , Shale Dennis J. 
Published: July 2022

AbstractThe Timed Up and Go (TUG) is a global measure of mobility and has the ability to detect frail individuals. Frail patients with chronic obstructive pulmonary disease (COPD) are usually undiagnosed. We hypothesised that the TUG would identify frail patients with COPD. Frailty was assessed in 520 patients diagnosed with COPD and 150 controls using a Comprehensive Geriatric Assessment questionnaire and frailty index (FI) was derived. The TUG was used to assess physical mobility. All participants were assessed for lung function and body composition. A ROC curve was used to identify how well TUG discriminates between frail and non-frail patients with COPD. The patients with COPD and controls were similar in age, sex and BMI but the patients with COPD were more frail, mean ± SD FI 0.16 ± 0.08 than controls 0.05 ± 0.03, P < 0.001. Frail patients with COPD had a greater TUG time (11.55 ± 4.03 s) compared to non-frail patients (9.2 ± 1.6 sec), after controlling for age and lung function (F = 15.94, P < 0.001), and both were greater than the controls (8.3 ± 1.2 sec), P < 0.001. The TUG discriminated between frail and non-frail patients with COPD with an area under the curve of 72 (95% CI: 67–76), and a diagnostic odds ratio of 2.67 (95% CI:1.5–4.6), P < 0.001. The TUG showed the ability to discriminate between frail and non-frail patients with COPD, independent of age and severity of the airflow obstruction. The TUG is a simple, easy and quick measure that could be easily applied in restricted settings to screen for frailty in COPD.
Effective pathogen removal in sustainable natural fiber Moringa filters
Authors: Samineni Laxmicharan , De Respino Sophie , Tu Yu-Ming , Chowdhury Ratul , Mohanty Rashmi Prava , Oh Hyeonji , Geitner Michael , Alberg Claire Hartwig , Roman-White Abigail , McKinzie Sarine , Lemus Camila , Massey Joy , Ghosh Debadyuti , Truskett Thomas M. , Velegol Stephanie , Kumar Manish 
Published: July 2022

AbstractPathogen contamination of water has a massive impact on global human health. In particular, viruses pose unique challenges to water treatment techniques due to their small size and presence in water as both individual virions and when absorbed onto larger particles. Low-energy water treatment processes such as media filtration are not capable of completely removing viruses owing to their small size. Hence, less sustainable processes with high chemical or energy consumption such as chemical disinfection, ultraviolet irradiation, and membrane filtration are usually required. To overcome high energy and/or chemical requirements for virus treatment, designs for sustainable fiber filters fabricated from minimally processed natural materials for efficient virus (MS2) and bacteria (E. coli) removal are presented in this work. These filters were created by functionalizing readily accessible natural fibers including cotton, silk, and flax with a simple aqueous extract containing cationic proteins from Moringa oleifera seeds. The proposed filters offer a comprehensive low cost, low energy, and low environmental impact solution for pathogen removal from water with removals of >7log10 (99.99999%) for viruses and bacteria.
A male steroid controls female sexual behaviour in the malaria mosquito
Authors: Peng Duo , Kakani Evdoxia G. , Mameli Enzo , Vidoudez Charles , Mitchell Sara N. , Merrihew Gennifer E. , MacCoss Michael J. , Adams Kelsey , Rinvee Tasneem A. , Shaw W. Robert , Catteruccia Flaminia 
Published: July 2022

AbstractInsects, unlike vertebrates, are widely believed to lack male-biased sex steroid hormones1. In the malaria mosquito Anopheles gambiae, the ecdysteroid 20-hydroxyecdysone (20E) appears to have evolved to both control egg development when synthesized by females2 and to induce mating refractoriness when sexually transferred by males3. Because egg development and mating are essential reproductive traits, understanding how Anopheles females integrate these hormonal signals can spur the design of new malaria control programs. Here we reveal that these reproductive functions are regulated by distinct sex steroids through a sophisticated network of ecdysteroid-activating/inactivating enzymes. We identify a male-specific oxidized ecdysteroid, 3-dehydro-20E (3D20E), which safeguards paternity by turning off female sexual receptivity following its sexual transfer and activation by dephosphorylation. Notably, 3D20E transfer also induces expression of a reproductive gene that preserves egg development during Plasmodium infection, ensuring fitness of infected females. Female-derived 20E does not trigger sexual refractoriness but instead licenses oviposition in mated individuals once a 20E-inhibiting kinase is repressed. Identifying this male-specific insect steroid hormone and its roles in regulating female sexual receptivity, fertility and interactions with Plasmodium parasites suggests the possibility for reducing the reproductive success of malaria-transmitting mosquitoes.
A transcriptomic axis predicts state modulation of cortical interneurons
Authors: Bugeon Stéphane , Duffield Joshua , Dipoppa Mario , Ritoux Anne , Prankerd Isabelle , Nicoloutsopoulos Dimitris , Orme David , Shinn Maxwell , Peng Han , Forrest Hamish , Viduolyte Aiste , Reddy Charu Bai , Isogai Yoh , Carandini Matteo , Harris Kenneth D. 
Published: July 2022

AbstractTranscriptomics has revealed that cortical inhibitory neurons exhibit a great diversity of fine molecular subtypes1–6, but it is not known whether these subtypes have correspondingly diverse patterns of activity in the living brain. Here we show that inhibitory subtypes in primary visual cortex (V1) have diverse correlates with brain state, which are organized by a single factor: position along the main axis of transcriptomic variation. We combined in vivo two-photon calcium imaging of mouse V1 with a transcriptomic method to identify mRNA for 72 selected genes in ex vivo slices. We classified inhibitory neurons imaged in layers 1–3 into a three-level hierarchy of 5 subclasses, 11 types and 35 subtypes using previously defined transcriptomic clusters3. Responses to visual stimuli differed significantly only between subclasses, with cells in the Sncg subclass uniformly suppressed, and cells in the other subclasses predominantly excited. Modulation by brain state differed at all hierarchical levels but could be largely predicted from the first transcriptomic principal component, which also predicted correlations with simultaneously recorded cells. Inhibitory subtypes that fired more in resting, oscillatory brain states had a smaller fraction of their axonal projections in layer 1, narrower spikes, lower input resistance and weaker adaptation as determined in vitro7, and expressed more inhibitory cholinergic receptors. Subtypes that fired more during arousal had the opposite properties. Thus, a simple principle may largely explain how diverse inhibitory V1 subtypes shape state-dependent cortical processing.