bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR167d-ARFs module regulates flower opening and stigma size in rice
Authors: Zhao Zhi-Xue , Yin Xiao-Xiao , Li Sha , Peng Yu-Ting , Yan Xiu-Lian , Chen Chen , Hassan Beenish , Zhou Shi-Xin , Pu Mei , Zhao Jing-Hao , Hu Xiao-Hong , Li Guo-Bang , Wang He , Zhang Ji-Wei , Huang Yan-Yan , Fan Jing , Li Yan , Wang Wen-Ming 
Published: May 2022

Abstract
Flower opening and stigma exertion are two critical traits for cross-pollination during seed production of hybrid rice (Oryza sativa L.). In this study, we demonstrate that the miR167d-ARFs module regulates stigma size and flower opening that is associated with the elongation of stamen filaments and the cell arrangement of lodicules, resulting in cleistogamy. The overexpression of miR167d (OX167d) resulted in failed elongation of stamen filaments, increased stigma size, and morphological alteration of lodicule. Blocking miR167 by target mimicry also led to a morphological alteration of the individual floral organs, including a reduction in stigma size and alteration of lodicule cell morphology, but did not show the cleistogamous phenotype. In addition, the four ARFs target genes of miR167d, namely ARF6, ARF12, ARF17, and ARF25, have overlapping functions in flower opening and stigma size. The loss-of-function of a single ARF gene did not influence the flower opening and stigma size, but arf12 single mutant showed a reduced plant height and aborted apical spikelets. However, mutation in ARF12 together with mutation in either ARF6, ARF17, or ARF25 led to the same defective phenotypes that were observed in OX167d, including the failed elongation of stamen filaments, increased stigma size, and morphological alteration of lodicule. These findings indicate that the appropriate expression of miR167d is crucial and the miR167d-ARFs module plays important roles in the regulation of flower opening and stigma size in rice.
Finite element modelling on Micro-machining of graphene reinforced aluminum matrix composites
Authors: Yu Hao , He Zhenpeng , Li Jinbo , Li Baichun , Xin Jia , Yao Lianzheng , Yan Fangchao 
Published: May 2022

Abstract
Graphene reinforced metal matrix composites have received a lot of attention from academia and industry due to their excellent mechanical properties. In this paper, the machinability of graphene reinforced aluminum metal matrix composites (Gr/Al MMCs) during micro-machining is investigated by finite element method. A three-dimensional modeling program based on the Python language is developed independently, this program can generate graphene with random distribution of orientation and position. A three-dimensional two-phase finite element model considering the cutting edge radius is established to simulate the micro-machining process of Gr/Al MMCs. Embedded element method is used in the model to more efficiently simulate the interaction behavior between graphene and aluminum matrix during micro-machining. The accuracy of the model is verified by indirect experiments. Stress distribution, tool-graphene interaction, chip formation, cutting force and specific cutting energy during micro-machining are investigated with different uncut chip thickness. The simulation results show that graphene inhibits stress propagation, resulting in the formation of a stress field with irregular contours within the matrix. Continuous serrated chips are generated during the cutting process. The addition of graphene increases the magnitude and fluctuation of cutting forces.
Clinical features and risk factors of plastic bronchitis caused by refractory Mycoplasma pneumoniae pneumonia in children: A nomogram prediction model
Authors: Zhang Han , Yang Jingjing , Zhao Wenqi , Zhou Jing , He Shuangyu , Shang Yunxiao , Cheng Qi 
Published: May 2022

Abstract
Background: Early assessment of refractory Mycoplasma pneumoniae pneumonia (RMPP) with plastic bronchitis (PB) allows timely removal of casts using fiberoptic bronchoscopic manipulation, which relieves airway obstruction and limit sequelae development. This study aimed to analyze clinical data for risk factors and develop a nomogram for early predictive evaluation of RMPP with PB.Methods: The clinical data of 1-14 year-old patients with RMPP were retrospectively analyzed. Patients were classified into a PB or non-PB group based on the presence or absence of PB as determined by large consolidation shadows on imaging and bronchoscopic findings. The general characteristics, clinical symptoms, laboratory test results, imaging findings, and microscopic changes of the two groups were compared. A statistical analysis of the risk factors for developing PB was performed, and a nomogram model of risk factors was constructed.Results: Of 120 patients with RMPP included, 68 and 52 were in the non-PB (control) and PB groups, respectively. Using multivariate logistic regression analysis, fever before bronchoscopy, extrapulmonary complications, pleural effusion, cough duration (days), and lactate dehydrogenase (LDH) levels were identified as risk factors. A nomogram was constructed based on the results of the multivariate analysis. The area under the receiver operating characteristic curve value of the nomogram was 0.944 (95% confidence interval: 0.779-0.962). The Hosmer-Lemeshow test displayed good calibration of the nomogram (p = 0.376, R2 = 0.723).Conclusions: The nomogram model constructed in this study based on five risk factors (persistent fever before bronchoscopy, extrapulmonary complications, pleural effusion, cough duration, and LDH levels) prior to bronchoscopy can be used for the early identification of RMPP-induced PB.
Deep Learning For Diabetic Retinopathy stage prediction
Authors: Garikapati Surua , Yarlagadda Venkata Saiteja 
Published: May 2022

Abstract
Diabetic Retinopathy (DR) is a prevalent issue among diabetic individuals, resulting in a constructive loss of vision in such people. If this problem is not diagnosed early on, there is no therapy available to restore vision. As a result, the only way out of this unreversible condition is to diagnose the sickness early on and cure it. The ophthalmologists use the "fundus images" of the patients' eyes, which are the retinal pictures of the patients, to maintain their eyesight. However, detecting an abnormality in a human eye with the naked eye of another person takes time, money, and can occasionally lead to misjudgment owing to subjective differences and concerns among ophthalmologists. As a result, we employ "Deep Learning" algorithms to diagnose diabetic retinopathy using fundus pictures in this article. As a result, a computer-aided diagnosis system is established, which leads to a reduction in misdiagnosis. Deep learning approaches have recently become the most popular ways for improving image recognition or feature detection systems' accuracy for both classification and regression. In this study, we employ Convolutional Neural Networks (CNN) for image identification, training the neural network model with retinal pictures, and achieving excellent accuracies. In this work, the difficulties of various methodologies as well as faults with existing methods were examined.
Identification of potential crucial genes in sarcopenia: a bioinformatic analysis
Authors: Su Youxiang , Li Nianhu , Xin Jian , Gao Shang , Song Yun , Qu Xintian , Liu Yan , Ma Xiusen 
Published: May 2022

Abstract
Background: Sarcopenia is a clinical syndrome characterized by continuous loss of skeletal muscle mass, muscle strength or function. Sarcopenia, which increases the risk of falls and fractures, is also closely associated with metabolic diseases and cognitive dysfunction, resulting in serious adverse effects on people’s quality of life and inflicting a heavy socio-economic burden. In the present study, we aim to identify potential crucial genes associated with sarcopenia through bioinformatic analyses of public datasets.Methods: The gene expression profiles (GSE1428) were downloaded from the Gene Expression Omnibus (GEO) database. The difference expression genes (DEGs) between sarcopenia samples and normal samples were screened. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the DEGs were performed. Protein-protein interaction (PPI) network was constructed using the STRING database. Cytoscape with CytoHubba were used to identify the hub genes.Results: Totally, 314 up-regulated genes and 289 own-regulated genes were identified. In addition, the most significantly enriched pathway was Cell cycle, Human T-cell leukemia virus 1 infection, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, Signaling pathways regulating pluripotency of stem cells. In combination with the results of the protein–protein interaction (PPI) network and CytoHubba, 10 hub genes including CD4、PLK1、CHEK2、LCP2、NANOG、PTPN11、RUNX2、ANXA5、BUB1B、CCR5 were selected. Conclusions: The 10 potential crucial genes may be associated with risk of sarcopenia. Our study provided new insights of sarcopenia into genetics, molecular pathogenesis and new therapeutic targets. It also will contribute to identification of potential biomarkers and novel strategies for sarcopenia.
Clinical Characteristics and Predictors of Adverse Outcomes at Discharge in Neonates with Bacterial Meningitis at a Tertiary Hospital in Suzhou, China
Authors: Wang Huawei , Gu Ying , Wu Zhixin , Zhu Xueping 
Published: May 2022

Abstract
Background: Neonatal bacterial meningitis is a severe infectious disease with a high rate of death and neurological complications. Early clinical signs and outcomes are different between preterm and full-term neonates, and little is known about their combined predictors. Therefore, this study aimed to explore the clinical characteristics and predictors of in-hospital outcomes in neonates with bacterial meningitis.Methods: We retrospectively analyzed hospitalized neonates with bacterial meningitis in the Department of Neonatology, Children’s Hospital of Soochow University from January 1,2013 to March 31, 2018. The clinical manifestations, laboratory data, treatment measures, neuroimaging and outcomes at discharge were recorded. Predictors of adverse outcomes were identified by using univariate analyses.Results: There were 145 neonates diagnosed with bacterial meningitis enrolling in this study, with 39 preterm and 106 full-term neonates. The incidence rate of neonatal bacterial meningitis was 0.56%. There were 28 neonates with birth weight < 2,500 g (19.31%), and 33 neonates presented with an onset of less than 7 days (22.76%). The predominant pathogens were Escherichia coli and Group B Streptococcus (accounting for 42.00% and 32.00%, respectively). The median peak fever during the acute illness in full-term neonates was higher than that in preterm neonates (p<0.05). The median platelet counts in peripheral blood and white blood cells in cerebrospinal fluid in full-term neonates were higher than those in preterm neonates (p<0.05). The positive rate of blood cultures in preterm neonates was higher than that in full-term neonates (71.79% vs. 49.06%, p=0.02). The incidence rates of adverse outcomes at discharge were 10/39 (25.64%) and 23/106 (21.69%) in preterm and full-term neonates, respectively. Preterm neonates with bacterial meningitis who had reduced glucose levels in cerebrospinal fluid were more likely to have adverse outcomes at discharge, while full-term neonates who had a bulging anterior fontanel, seizure/convulsions and reduced glucose levels in cerebrospinal fluid were more likely to have worse outcomes.Conclusions: The incidence rate of hospitalized neonates associated with bacterial meningitis remains high. Escherichia coli and Group B Streptococcus were the predominant pathogens in neonatal bacterial meningitis in our region. The risk factor for an adverse outcome at discharge in preterm neonates with bacterial meningitis was a lower cerebrospinal fluid glucose concentration, meanwhile, the risk factors in full-term neonates were a bulging anterior fontanel, seizure/convulsions, and a lower cerebrospinal fluid glucose concentration.
Expression and prognostic value of Melanoma-associated antigen D2 in gliomas
Authors: Yan Jun , Li Shenyu , Lenahan Cameron , Fu Kaijing , Mo Yong , Chen Hainan , Wen Jing , Huang Qianrong , Wei Zongdang , Jiang Qian , Guo Fangzhou , Deng Teng , Mo Ligen 
Published: May 2022

Abstract
Introduction The melanoma-associated antigen D4 (MAGED2) is one of the melanoma-associated antigen family members. It is commonly overexpressed in a variety of malignancies. However, the mechanism and function of MAGED2 in glioma remain unknown. Methods The TCGA and Oncomine databases were used to determine the expression level of MAGED2 and its relationship to glioma. MAGED2 protein expression in 98 tumor tissues from glioma patients was measured using RT-qPCR, Western blot and immunohistochemistry, and the associations between MAGED4 expression and clinicopathological factors were evaluated. qRT-PCR and Western blots were used to assess the levels of MAGED2 and CDKN1A expression in glioma U251-MG cells following transfection of MAGED2 CRISPR. CCK-8, colony formation, and EdU assays were used to assess the effect of MAGED2 on U251-MG cell proliferation, and flowcytometry was used to track changes in the cell cycle and cell apoptosis following MAGED2 CRISPR transfection. Results In the current investigation, MAGED2 was shown to be substantially expressed in human glioma tissues, and high MAGED2 expression predicted poor recurrence-free survival (RFS) and overall survival (OS) for glioma patients. The presence of high MAGED2 expression was related to WHO grade, Ki-67, IDH1/2, MGMT, and survival status. Furthermore, MAGED2 expression knockdown significantly inhibited the proliferation and colony formation potential of U251-MG cells by preventing cell cycle arrest at the G0/G1 phase and triggering apoptosis. Moreover, our findings indicated that the mRNA and protein expression levels of CDKN1A were considerably up-regulated in vitro following MAGED2 CRISPR transfection. MAGED2 downregulation inhibited the ability of cell proliferation in the U251-MG cells through restoring CDKN1A. Conclusions To conclude, the current study's findings imply that a high level of MAGED2 expression predicts a poor prognosis for glioma patients. MAGED2 can stimulate the proliferation of glioma U251-MG cells by targeting CDKN1A. MAGED2 may be a possible biomarker for glioma and an important prognostic factor for glioma patients.
Elevated eosinophils as predictor of immune-related adverse events after ipilimumab and nivolumab treatment of advanced and metastatic renal cell carcinoma： a multicenter cohort study Eosinophil could be biomarkers for irAE
Authors: Tasaki Yoshihiko , Hamamoto Shuzo , Sugiyama Yosuke , Tomiyama Nami , Naiki Taku , Etani Toshiki , Taguchi Kazumi , Iwatsuki Shoichiro , Matsuyama Nayuka , Sue Yasuhito , Mimura Yoshihisa , Odagiri Kunihiro , Kubota Hiroki , Noda Yusuke , Hashimoto Yoshihiro , Sakakura Tsuyoshi , Aoki Maria , Ikegami Yosuke , Kobayakawa Yuki , Moritoki Yosinobu , Nozaki Satoshi , Takeda Tomoki , Kurokawa Satoshi , Okada Atsushi , Kawai Noriyasu , Yasui Takahiro , Kimura Kazunori 
Published: May 2022

Abstract
Purpose: Ipilimumab and nivolumab treatment against advanced and metastatic renal cell carcinoma (RCC) causes severe and lethal immune-related adverse events (irAEs). Predicting irAEs might improve clinical outcomes, however no practical biomarkers exist. This study examined whether eosinophils could be effective biomarkers for irAEs in RCC.Methods: We retrospectively analyzed 75 patients with RCC treated with ipilimumab and nivolumab between August 2018 and March 2021 in a multicenter study. Eosinophils were examined before and two weeks after treatment, and immediately after irAE development. Median overall (mOS) and progression-free (mPFS) survival were examined by Kaplan–Meier method. The optimal cut-off value for irAE was determined by a receiver operating characteristic (ROC) curve. Univariate and multivariate analyses were undertaken to identify predictors of irAEs.Results: The mOS and mPFS of patients who experienced irAEs (irAE group) were longer than those of the non-irAE group. Grade ≧2 irAEs were associated with poor mPFS. The eosinophil level two weeks after treatment was significantly upregulated in the irAE compared to non-irAE group (mean, 3.0% vs. 5.7%; P < 0.05). The ROC curve revealed the optimal cut-off value for eosinophil levels against ≧grade 2 irAE two weeks after treatment was 3.0% (area under the curve=0.699). In multivariate analyses, an eosinophil level ≧3.0% was a risk factor for ≧grade 2 irAE (odds ratio 4.18, 95% confidence interval 1.16–15.1).Conclusion: An increased eosinophil level two weeks after treatment might be an effective biomarker for irAEs in patients with RCC treated with ipilimumab and nivolumab.
Intrascleral four-flanged intraocular lens fixation technique for lens dislocation with Marfan syndrome
Authors: zhou li , wang yong 
Published: May 2022

Abstract
Purpose
To observe the clinical efficacy of intrascleral four-flanged intraocular lens(IOL) fixation technique for lens dislocation with Marfan syndrome.
Methods
A retrospective analysis Observed twelve patients with Marfan syndrome(15 eyes) with lens dislocation who had underwent this operation(A 30g needle was used to puncture into the eye at 2, 4 o'clock or 8, 10 o'clock from the outside of the eye, respectively,.Both ends of the 7 − 0 polypropylene thread were guided through the two eyelets on the ipsilateral side of the Akreos AO IOL and out of the eyeball,and then the two ends of the suture were borned to form flanges. Finally two pairs (four) flanges are formed at 2,4 o'clock and 8,10 o'clock, which were riveted within the scleral tunnel, so as to fix the IOL). Corrected distance visual acuity(CDVA), intraocular pressure(IOP), tilt and decentration of the IOL and postoperative complications were evaluated.
Results
All operations were completed successfully. The CDVA prior to the fixation surgery was 3.36 ± 0.886 logMAR and on the first day aftet the surgery was 4.12 ± 0.928 logMAR. The CDVA was significantly improved following surgery (t = 0.660, p = 0.029). The CDVA on the last visit was 4.48 ± 0.736 logMAR, Compared with the first day after surgery, the difference is not statistically significant (t = 0.332, p = 0.249). No intraoperative complications were reported. Postoperatively, elevated IOP 4h after surgery was recorded in 6 eyes (40.0%), cystoid macular edema (CME) was seen in 2 eyes (13.33%), vitreous hemorrhage and flange exposure were seen in one eye each, corneal edema and anterior chamber inflammation were seen in 4 eyes each. The IOLs were centered without tilt and decentration.
Conclusions
The intrascleral four-flanged IOL for lens dislocation with Marfan syndrome experienced a low incidence of complication with excellent visual outcome. This technique is a simple, effective, and safe method.
Assessment of ecosystem health status using benthic diversity as tools in coastal sediment off the southwest coast, India
Authors: Arya DB , Godson PS , Selvaraj P , Krishnakumar S , Magesh NS , Vincent Salom Gnana Thanga 
Published: May 2022

Abstract
The diversity of macro and meiobenthos was documented in order to assess the ecosystem health of an anthropogenically impacted coastal ecosystem for the first time along the southwest coast of Tamil Nadu. Offshore sediment and water samples (n=63) were collected from 14 sampling stations using the grid sampling method. Water and sediment samples were analysed for environmental variables such as pH, temperature, electrical conductivity (EC), total dissolved solids (TDS), salinity, sulphate, dissolved oxygen (DO), carbohydrate (CHO), protein, lipid, labile organic matter (LOM), total organic carbon (Corg), total organic matter (TOM) and sediment texture using standard methods. Benthic macro and meiobenthos were isolated and identified using standard identification keys. A total of 5 phyla of macrobenthos with 29 species and 5 phyla of benthos with 60 species were identified in the study area. Relative abundance analysis showed that the largest numbers of species belonged to polychaeta for macrobenthos and foraminifera for meiobenthos. Redundancy analysis indicated that sediment carbohydrate content was the highest determining factor for explaining variation in the diversity of benthos. AMBI & M-AMBI results revealed that among the 14 stations, 2 were categorized as “poor” and 9 as “bad”. Multiple anthropogenic activities in the coastal environment leading to increased organic matter input, have resulted in a decline in the ecosystem health of this region which warrants for site-specific management to control organic pollution.
Covid 19 Make us Confident to Perform ESWL Sessions for Treatment of Ureteric Stone
Authors: Memon Waqar Ahmed , Arain Adeel , Jat Javed Altaf , Arain Ahsan , Abid Shariq , Jaffry Ali Raza , Mal Pooran 
Published: May 2022

Abstract
The clinicians prefer URS as a first-line line treatment of upper ureteric stones but with COVID-19 restrictions and considering the patient's benefit, ESWL sessions to remove ureteric stones were preferred as first-line treatment of upper ureteric stones. The aims of this study was to compare the level of stone clearance with EWSL sessions during COVID-19 pandemic as a first-line treatment. A longitudinal study was conducted at the Department of Urology, Liaquat University Hospital for the duration of 6 months. Total thirty patients of age more than 15 years who presented with an upper ureteric stone of 0. Patients were managed for upper ureteric stone with ESWL and complete stone clearance was considered a satisfactory outcome. The mean age of patients enrolled in the study was 31 10. 82 years with a preponderance of males that is 55%. The mean size of the stone observed was 1.10 0. Upon exposure to EWSL sessions, 68% of patients showed complete clearance of stones whereas 10% of patients had no clearance and 16% of patients showed partial clearance. The comparison of different variables among the level of stone clearance showed that complete removal of stone was carried out by a maximum of 4 EWSL sessions having a mean stone size of 1.09 0. 22 cm whereas maximum of 3 sessions were given to patients that resulted in incomplete stone clearance. In conclusion, the decision for carrying out EWSL sessions for ureteric stone clearance was successful in the majority of patients during a pandemic.