bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diffuse sclerosing variant of papillary thyroid carcinoma: outcomes of 33 cases
Authors: Cavaco Daniela , Martins Ana Filipa , Cabrera Rafael , Vilar Helena , Leite Valeriano 
Published: January 2022

Introduction
Diffuse sclerosing variant of PTC (DSV-PTC) is an uncommon subtype of thyroid cancer. Although an aggressive behavior is often recognized, prognostic significance is still under debate.

Objectives
To describe the clinicopathological features and outcomes of a series of DSV-PTC patients.

Methods
Retrospective data collection involving 33 patients diagnosed with DSV-PTC followed at the Endocrine Department of the Portuguese Institute of Oncology in Lisbon between 1981 and 2020.

Results
Twenty-six patients (78.8%) were females with a mean age at presentation of 29.4 ± 11.7 years old. Mean time of follow-up was 19.5 ± 10.6 years (range 0.5–39). Histologically, bilateral tumors were present in 72.7% patients (n  = 24), thyroid capsular invasion was documented in 57.6% (n  = 19), 45.4% (n  = 15) had extrathyroidal extension, and 42.4% (n  = 14) had lymphovascular invasion. Most patients were staged pT3 (42.4%, n  = 14) and pN1 (81.8%, n  = 27). Median lymph nodes resected were 16. None of the patients showed distant metastases at presentation. All patients were treated at least once with 131I. During follow-up, four patients (14.8%), with persistent neck disease, were diagnosed with distant metastases, all of them in the lung. Two patients (1.8%) presented recurrent disease in the neck after being considered with no evidence of disease. At the last appointment, 18 patients (54.5%) were in remission, 4 (12.1%) had biochemical evidence of disease, 6 had structural disease, and for 5 patients disease status was considered as undetermined. There was no disease related mortality.

Discussion/conclusion
Our study confirms that DSV-PTC is diagnosed more often in young patients and exhibits a local extensive disease at presentation. On the other hand, even in the presence of distant metastases, no patient died during follow-up.
Iodine supplementation: compliance and association with adverse obstetric and neonatal outcomes
Authors: Lopes-Pereira Maria , Quialheiro Anna , Costa Patrício , Roque Susana , Correia Santos Nadine , Correia-Neves Margarida , Goios Ana , Carvalho Ivone , Korevaar Tim I M , Vilarinho Laura , Palha Joana Almeida 
Published: January 2022

Objectives
Over 1.9 billion people worldwide are living in areas estimated to be iodine insufficient. Strategies for iodine supplementation include campaigns targeting vulnerable groups, such as women in pre-conception, pregnancy and lactation. Portuguese women of childbearing age and pregnant women were shown to be mildly-to-moderately iodine deficient. As a response, in 2013, the National Health Authority (NHA) issued a recommendation that all women considering pregnancy, pregnant or breastfeeding, take a daily supplement of 150–200 μg iodine. This study explored how the iodine supplementation recommendation has been fulfilled among pregnant and lactating women in Portugal, and whether the reported iodine supplements intake impacted on adverse obstetric and neonatal outcomes.

Design and methods
Observational retrospective study on pregnant women who delivered or had a fetal loss in the Braga Hospital and had their pregnancies followed in Family Health Units.

Results
The use of iodine supplements increased from 25% before the recommendation to 81% after the recommendation. This was mostly due to an increase in the use of supplements containing iodine only. Iodine supplementation was protective for the number of adverse obstetric outcomes (odds ratio (OR) = 0.791, P = 0.018) and for neonatal morbidities (OR = 0.528, P = 0.024) after controlling for relevant confounding variables.

Conclusion
The recommendation seems to have succeeded in implementing iodine supplementation during pregnancy. National prospective studies are now needed to evaluate the impact of iodine supplementation on maternal thyroid homeostasis and offspring psychomotor development and on whether the time of the beginning of iodine supplementation (how early during preconception or pregnancy) is relevant to consider.
Impact of body mass index on short-term outcomes after differentiated thyroid cancer surgery: a nationwide inpatient database study in Japan
Authors: Konishi Takaaki , Fujiogi Michimasa , Michihata Nobuaki , Niwa Takayoshi , Morita Kojiro , Matsui Hiroki , Fushimi Kiyohide , Tanabe Masahiko , Seto Yasuyuki , Yasunaga Hideo 
Published: January 2022

Introduction
Recent studies have shown worse post-operative outcomes following several surgeries in underweight or obese patients. However, the association between body mass index (BMI) and short-term outcomes following thyroid cancer surgery remains unclear because of the small number of patients, deficits in background data known as risk factors (e.g. cancer stage, operative procedure, intraoperative device use and hospital volume) and categorisation of BMI.

Methods
We identified patients who underwent thyroidectomy for differentiated thyroid cancer from July 2010 to March 2017 using a Japanese nationwide inpatient database. We used restricted cubic spline (RCS) analyses to investigate potential non-linear associations between BMI (without categorisation) and outcomes: post-operative complications (local and general), duration of anaesthesia, post-operative length of hospital stay and hospitalisation costs. The analyses were adjusted for demographic and clinical backgrounds including the above-stated factors. We also performed multivariable regression analyses for the outcomes with categorisation of BMI.

Results
Among 59,671 eligible patients, the median BMI was 22.9 kg/m2 (interquartile range (IQR), 20.7–25.6 kg/m2). In total, 3860 patients (6.5%) had local complications and 787 patients (1.3%) had general complications. Although there were no significant associations with local complications, such as bleeding, recurrent laryngeal nerve paralysis and surgical site infection, the occurrence of general complications was significantly associated with higher BMI. BMI showed a linear association with the duration of anaesthesia and U-shaped associations with post-operative length of stay and hospitalisation costs. The lowest points of the U-shaped curves occurred at a BMI of approximately 24 kg/m2. The multivariate regression analyses showed consistent results with the RCS analyses.

Discussion/conclusion
Whereas RCS analyses revealed no significant associations between BMI and post-operative local complications, obesity was significantly associated with the occurrence of general complications. The linear association between BMI and duration of anaesthesia corresponds to previous studies. Although post-operative length of stay and total hospitalisation costs demonstrated U-shaped associations, the slight differences would not be clinically important. Even if surgeons must pay attention to general complications in obese patients undergoing thyroid cancer surgery as well as other surgeries, underweight and overweight patients can undergo thyroidectomy as safely as patients with normal BMI.
ФІНАНСОВИЙ МЕХАНІЗМ КАПІТАЛІЗАЦІЇ ЗЕМЕЛЬНИХ РЕСУРСІВ СІЛЬСЬКОГОСПОДАРСЬКИХ ПІДПРИЄМСТВ РЕГІОНУ У ВЗАЄМОВІДНОСИНАХ ІЗ ТЕРИТОРІАЛЬНИМИ ГРОМАДАМИ
Authors: Levandivskyi O.T. , Balaniuk I.F. , Shelenko D.I. , Sas L.S. 
Published: January 2022

У статті розглянуто фінансовий механізм капіталізації земельних ресурсів сільськогосподарських підприємств у взаємовідносинах з територіальними громадами, етапи його формування. Акцентовано увагу на необхідності ринкової капіталізації земель сільськогосподарського призначення у взаємовідносинах з територіальними громадами. Розглядається й аналізуються методичні підходи у грошовій оцінці земельних ресурсів. Відображено нормативну грошову оцінку земель сільськогосподарського призначення в Україні за сільськогосподарськими угіддями. Проведено аналіз орендної плати щодо використання земель сільськогосподарського призначення за регіонами.
Досліджено основні види оцінки земельних ресурсів залежно від мети та методів її проведення. Здійснено аналіз нормативно-правових актів, які регламентують порядок проведення оцінки земельних ресурсів. З’ясовано, що вартість, за якою земельні ресурси відображаються в обліку залежить від способу набуття їх у власність та/або користування, а також від мети їх використання. Встановлено, що в бухгалтерському обліку для оцінки земельних ресурсів використовуються такі види вартостей, як: первісна (історична (фактична) собівартість або справедлива вартість) та переоцінена.
У процесі дослідження використано такі основні методи: системно-структурний та абстрактнологічний (для теоретичних узагальнень результатів аналітичних розрахунків і формулювання висновків); аналізу і синтезу (для деталізації об’єкта дослідження через його поділ на окремі складові та зведення результатів їх вивчення в одне ціле); метод порівняльного аналізу (для обґрунтування застосування окремих інструментів регулювання виробничих і управлінських відносин); економіко-статистичний – групування, кореляційно-регресійний аналіз (для виявлення впливу чинників на окремі його критерії); розрахунковоконструктивний (в опрацюванні стратегії розвитку).
Доведено, що з формуванням ринку земель питання з нормативної грошової оцінки земель сільськогосподарського призначення набувають нового змісту та потребують подальшого дослідження. Звернута увага на взаємовідносини територіальних громад і сільськогосподарських підприємств щодо наповнення місцевого бюджету платою за використання земельних ресурсів. Визначено капіталізацію земельних ресурсів з поступовою трансформацією їх у капітал задля підвищення ринкової ціни.
Виявлено значну диференціацію вартості сільськогосподарських земель у регіонах України за нормативно грошовою оцінкою. Зосереджено увагу на значній недооцінці земельних ресурсів та довгому терміні їх капіталізації. Із метою трансформації земельних ресурсів у фінансові активи обґрунтовано пропозиції щодо удосконалення фінансового механізму капіталізації земельних ресурсів в аграрному секторі. Зокрема, наголошується на системі експертної та нормативно грошової оцінки земель, формування та розвитку інфраструктури ринку сільськогосподарських земель.
Non-symmetric approximations of functional classes by splines on the real line
Authors: Parfinovych N.V. 
Published: January 2022

Let $S_{h,m}$, $h>0$, $m\in {\mathbb N}$, be the spaces of polynomial splines of order $m$ of deficiency 1 with nodes at the points $kh$, $k\in {\mathbb Z}$.
We obtain exact values of the best $(\alpha, \beta)$-approximations by spaces $S_{h,m}\cap L_1({\mathbb R})$ in the space $L_1({\mathbb R})$ for the classes $W^r_{1,1}({\mathbb R})$, $r\in {\mathbb N}$, of functions, defined on the whole real line, integrable on ${\mathbb R}$ and such that their $r$th derivatives belong to the unit ball of $L_1({\mathbb R})$.
These results generalize the well-known G.G. Magaril-Ilyaev's and V.M. Tikhomirov's results on the exact values of the best approximations of classes $W^r_{1,1}({\mathbb R})$ by splines from $S_{h,m}\cap L_1({\mathbb R})$ (case $\alpha=\beta=1$), as well as are non-periodic analogs of the V.F. Babenko's result on the best non-symmetric approximations of classes $W^r_1({\mathbb T})$ of $2\pi$-periodic functions with $r$th derivative belonging to the unit ball of $L_1({\mathbb T})$ by periodic polynomial splines of minimal deficiency.
As a corollary of the main result, we obtain exact values of the best one-sided approximations of classes $W^r_1$ by polynomial splines from $S_{h,m}({\mathbb T})$. This result is a periodic analogue of the results of A.A. Ligun and V.G. Doronin on the best one-sided approximations of classes $W^r_1$ by spaces $S_{h,m}({\mathbb T})$.
Pengaruh Penambahan Gliserol dan Kitosan Kulit Udang Terhadap Biodegradasi dan Ketahanan Air Plastik Biodegradable
Authors: Solekah Susi , Sasria Nia , Dewanto Hizkia Alpha 
Published: January 2022

Pada plastik biodegradable, aktivitas mikroorganisme menyebabkan plastik jenis ini dapat terdegradasi sehingga tidak berbahaya bagi lingkungan. Pada penelitian ini plastik dibuat dari bahan dasar pati kulit singkong. Untuk meningkatkan karakteristik dari plastik berupa fleksibilitas dibutuhkan penambahan gliserol guna menghasilkan plastik yang fleksibel, dengan memvariasikan gliserol dengan komposisi 2 mL, 4 mL dan 6 mL. Untuk meningkatkan sifat mekanik dari plastik dibutuhkan penambahan kitosan, dengan memvariasikan kitosan dengan komposisi 0,5 g, 1 g dan 1,5 g. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh dari gliserol dan kitosan terhadap kemampuan biodegradasi dan ketahanan air dari plastik biodegradable serta kemampuan biodegradasi dari plastik biodegradable dalam waktu 7 hari. Pada penelitian ini menggunakan metode pengujian berupa uji FTIR untuk mengetahui gugus fungsi pada sampel, uji ketahanan air (uji swelling) untuk mengetahui ketahanan air pada sampel, uji biodegradasi mengetahui kemampuan degradasi dari sampel dan uji mikroskop optik untuk mengidentifikasi struktur mikro dari sampel. Pada penelitian ini untuk hasil biodegradasi yang paling tinggi yaitu pada sampel dengan variasi 0,5 g kitosan dan 2 mL gliserol sebesar 35.43% dan untuk hasil biodegradasi yang paling rendah yaitu pada sampel dengan variasi 2 g kitosan dan 6 mL gliserol yaitu sebesar 12.20% sedangkan untuk hasil uji ketahanan air (uji swelling) yang paling tinggi yaitu pada sampel dengan variasi kitosan 0,5 g kitosan dan 2 mL gliserol sebesar 50.59% dan hasil yang paling rendah yaitu pada sampel dengan variasi 1 g kitosan dan 2 mL gliserol yaitu sebesar 28.90%.
Analisis Bioinformatika dan Ekspresi Protein Rekombinan Hemagglutinin Domain Globular dari Virus H5N1 Indonesia pada Eschericia coli BL21 (DE3) sebagai Komponen Vaksin Subunit Influenza
Authors: Yusuf Muhammad , Handiyani Rima , Kusumawardani Shinta , Idar Idar , Baroroh Umi , Subroto Toto 
Published: January 2022

Flu burung merupakan salah satu penyakit zoonosis yang patut diwaspadai di Indonesia, khususnya galur High Pathogenic Avian Influenza (HPAI) karena dapat mematikan jika menular kepada manusia. Penggunaan vaksin influenza pada unggas, merupakan langkah preventif terhadap evolusi virus yang berbahaya dan juga penyebarannya. Selama ini, Indonesia masih menggunakan seed vaksin impor yang berasal dari luar Indonesia. Namun, karena Indonesia merupakan negara yang berada di garis khatulistiwa, karakteristik virus bisa berbeda dengan virus dari nothern-hemispere maupun southern-hemispere. Mengingat hal tersebut, Indonesia harus mengembangkan vaksin influenza menggunakan galur virus lokal. Berbeda dengan vaksin whole virus, vaksin rekombinan memiliki keunggulan dari sisi kemurnian, kecepatan produksi, dan kesesuaian galur terhadap virus yang beredar saat diperlukan. Penelitian ini bertujuan untuk menganalisis sekuen hemagglutinin (HA) Indonesia dengan strain lainnya serta mengekspresikkan protein HA1 rekombinan pada Escherichia coli BL21 (DE3). Galur yang digunakan pada studi ini berasal dari virus H5N1 (A/Indonesia/05/05), khususnya bagian domain globular dari HA1. Sekuen HA1 bervariasi antara strain Indonesia dengan nothern-hemispere maupun southern-hemispere dan merupakan protein yang terpapar ke luar virus. Gen HA1 disisipkan pada vektor pET-28a, kemudian plasmid diisolasi menggunakan meoe manniatis, setelah itu diekspresikan dengan induksi 1 mM IPTG selama 4 jam. Protein HA1 telah berhasil diekspresikan secara intraseluler dan telah dikonfirmasi pada berat molekul 40 kDa menggunakan SDS-PAGE. Penelitian ini dapat digunakan untuk mengembangkan vaksin subunit yang lebih spesifik terhadap virus yang beredar di lapangan.
Prospects for the transition of the textile industry in the Russian to a closed-loop economy
Authors: Dolgushin A. B. , Tsukanov A. A. , Petrov A. D. 
Published: January 2022

The life of a modern person today cannot be imagined free from an urgent and urgent problem – the problem of waste recycling. At the same time, the environmental situation in large cities, including industrial enterprises, is mainly determined by the state of the waste treatment system. These include solid municipal waste, as experts call the garbage that is formed daily in everyday life and makes a long way from the garbage chute to the yard container and further to the city dump.At the moment, the infrastructure for collecting and processing secondary raw materials (glass, plastic, paper, batteries, etc.) is developing on the territory of many CIS countries, including Russia, while there are no reception points for textile waste of production and consumption in sufficient quantities, unlike in European countries. There are retail outlets that accept unnecessary textiles from the population, and in return provide discounts on their products. However, taking into account the volume of textile waste generation (about 4.2–4.8 million tons per year), the existing textile waste collection system, which collects only about 0.4% of textile waste, is clearly insufficient. Based on the identified problems, the authors of this article give a socio-ecological and economic assessment of the problem of handling textile waste of consumption in Russia. The analysis of the relationship of the problems of textile waste management to the UN Sustainable Development Goals is carried out. The international experience of applying the principles of the closed-cycle economy in relation to textile waste is considered and recommendations for the transition of the Russian textile industry to a closed-cycle economy are formed.
Neuromuscular disease - Gene transfer for children
Authors: Martin Matthew , Ream Margie , Kuntz Nancy , Mathews Katherine , Connolly Anne 
Published: January 2022

Successful gene transfer therapy (GTT) provides a functional copy of a gene to appropriate tissues for affected patients. While technically difficult, GTT holds great promise for treating and even curing previously fatal diseases.
GTT for Spinal Muscular Atrophy is available commercially and ongoing studies continue to show it is safe and effective. Subclinical liver dysfunction is more common in older, heavier children receiving higher vial loads. Human trials support preclinical studies showing early timing of therapy is important.
GTT for Duchene Muscular Dystrophy has required strategic approaches to create mini- and micro-dystrophin genes that will fit into available viral vectors. There are multiple ongoing studies that overall demonstrate good safety and efficacy.
GTT for X-Linked Myotubular Myopathy is being studied in an ongoing trial that has shown improvement in respiratory function (including ventilator independence), neuromuscular function, and histopathological evaluation. Three patients with severe cholestatic liver dysfunction have died. Evaluation is ongoing to better understand these events.
While GTT for neuromuscular disorders holds significant promise, it is not without risks and requires in-depth knowledge of the disease, abundant pre-clinical work, careful patient education, and ongoing patient care. There are a number of key questions that must be considered regarding the feasibility of expanding GTT to new disorders
These examples illustrate how advances in GTT benefit children on a population level and may themselves benefit from early detection by NBS. By becoming involved in advocacy at state and federal levels, families and physicians can impact newborn screening policy and implementation regarding these disorders.
OPTIMIZING THE PRODUCTION OF A FUNCTIONAL TYPE A RECOMBINANT ENDOCHITINASE FROM Trichoderma asperellum IN Escherichia coli
Authors: Luong Nguyen Ngoc , Tien Nguyen Quang Duc , Hoa Phung Thi Bich , Tue Nguyen Hoang , Hien Mai Thi Thu , Loc Nguyen Hoang , Huy Nguyen Xuan 
Published: January 2022

Chitinases from the genus Trichoderma fungi are mainly responsible for their anti-fungal activities, which allow them to become the most widely used fungal biocontrol. Therefore, several Trichoderma chitinases have been cloned and expressed to facilitate their production and applications. A previous study of the same authors has characterized an endochitinase from a relatively novel Trichoderma spp., Trichoderma asperellum. To produce this enzyme more economically and efficiently, we reported the synthesis and expression of its synthetic encoding gene in the Escherichia coli M15 strain and established the optimal conditions for preparative scale production of the enzyme in its functional form. By lowering the induction temperatures, we observed substantial improvement in the expression levels of the active enzyme. At 30 oC and 0.5 mM IPTG induction, 1 L of cells yielded approximately 80 - 100 mg of soluble protein, accounting for about 9-11 % of total soluble protein. This figure may be an underestimation of the actual yield, as deduced from the SDS-PAGE data. The recombinant enzyme can be retrieved by simple repeated freezing and thawing cycles and purified to near homogeneity using Ni-NTA chromatography. The purified enzyme showed in vitro colloidal chitin hydrolysis activity. These results could be scaled up to produce soluble 42 kDa chitinase in E. coli. The study demonstrated an economical method to produce chitinases for various agricultural and environmental applications.
Bermain aplikasi gadget berhubungan dengan keterlambatan perkembangan bicara dan bahasa pada balita
Authors: Suryawan Kadek Bayu , Merijanti Lie Tanu 
Published: January 2022

LATAR BELAKANGGadget merupakan salah satu media informasi dan teknologi yang berkembang pesat dan telah membuat penggunanya menjadi ketergantungan, bahkan balita. Penggunaan gadget secara berlebihan dan tidak tepat dapat menimbulkan dampak berupa keterlambatan bicara atau bahasa. Di Indonesia jumlah keterlambatan bicara dan bahasa pada balita mencapai 10%. Tujuan penelitian ini untuk mengetahui hubungan intensitas penggunaan gadget dengan keterlambatan perkembangan aspek bicara dan bahasa pada balita.METODEPenelitian ini menggunakan metode analitik observasional dengan pendekatan cross-sectional terhadap 100 responden yang memenuhi kriteria inkulsi maupun eksklusi. Pengumpulan data dilakukan melalui wawancara menggunakan kuesioner intensitas penggunaan gadget dan kuesioner pra skrining perkembangan pada bulan Oktober–Desember 2019 di TK Kelurahan Tomang, Jakarta Barat. Analisis data dilakukan dengan uji Chi-square dengan tingkat kemaknaan yang digunakan p<0.05.HASILDengan total 100 responden, didapatkan 18 orang (18%) intensitas penggunaan gadget rendah, 29 orang (29%) intensitas penggunaan gadget sedang dan 53 orang (53%) dengan intensitas penggunaan gadget tinggi. Responden dengan keterlambatan perkembangan aspek bicara dan bahasa sebanyak 39 orang (39%) dan 61 orang (61%) lainnya tidak mengalami keterlambatan perkembangan aspek bicara dan bahasa. Hasil analisis Chi-square menunjukkan hubungan intensitas penggunaan gadget dengan keterlambatan perkembangan aspek bicara dan bahasa pada balita memiliki nilai signifikansi (p=0.002).KESIMPULANIntensitas penggunaan gadget berhubungan dengan keterlambatan perkembangan aspek bicara dan bahasa pada balita.