bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quarantine and antibiotic susceptibility of Enterobacteriaceae strains and other gram negative bacteria in dairy sweetmeat milk (doodh) peda
Authors: Amreen Fouzia , Puttananjaiah Mohan kumari Honganoor , Viswanath Prema 
Published: August 2022

Abstract
The aim of the present study was to assess the prevalence and isolation of Enterobacteriaceae strains and other gram negative bacteria of milk (doodh) peda-a traditional milk product of India. Bacteria of the family Enterobacteriaceae are the indicator organisms used to provide evidence of poor hygiene, inadequate processing or post-process contamination of foods. A total of 25 peda samples were collected from different places of Mysuru district to evaluate the prevalence of Enterobacteriaceae. Seventeen Gram negative fermentative bacteria of clinical significance were detected, isolated and subsequently identified using API 20E strips method and other associated biochemical tests. Our outcomes indicate that milk peda were contaminated with the Enterobacteriaceae like Enterobacter cloacae, Yersinia bercovieri, Yersinia rohdei, Raoutlella terigena, Acinetobacter lwoffii, Pantoea agglomerans PA2, Klebsiella pneumoniae subsp pneumoniae, Leclericia adecarboxylata, Photorhabdus nematophilus, Enterobacter gergoviae, Acinetobacter baumannii/calcoacetius, Cronobacter sakazaki (Enterobacter sakazaki). Further, the antimicrobial resistant of these Enterobacteriaceae group were also investigated against 20 antibiotics by the disc diffusion assay method. All isolates revealed susceptibility to the fluoroquinones and aminoglycosides, but resistance to the nitrofurantoin (70.59%), augmentin (52.94%), and cefpodoxime (47.06%) antibiotics. Our finding was the first report of prevalence and detection of E. gergoviae in a food sample (milk peda) and examined for antibiotic resistances.
Psychometric properties of an instrument to measure perception of aphrodisiac use among undergraduates in a Southwestern Nigerian University
Authors: Fatade Olawunmi Cecilia , Makinde Gabriel Ifeoluwa , Adebayo Ayodeji Matthew 
Published: August 2022

Abstract

A tool to measure perception of aphrodisiac use by university institution undergraduates was developed and validated. The study was hinged on several theories that could explain potential to use aphrodisiac among the youths. A cross-sectionally designed study that evolved from qualitative and quantitative processes deployed a pretested instrument adapted from literature reviews and expert judgement on 919 students of University of Ibadan sampled through multi-stage method. Exploratory factor analysis was deployed on Polychoric correlation matrix of the items using R-statistical packages. Further model fit analysis was conducted using Confirmatory factor analysis on the items and factors derived from EFA. Mean Z-scores of factors were computed against socio-demographics and symptoms of aphrodisiac use among respondents that have ever used it. Two succession of factor analyses and a structural equation modeling suggested that a first-order model is good fit for experimental data (TLI = 0.931; CFI = 0.948; SRMR = 0.047; RMSEA = 0.083). The four-factor solution to the model included: prolonged sexual performance, use without erectile dysfunction or medical advice, treatment of erectile dysfunction and recreational purposes with an internal consistency that ranged from 0.62–0.92. The validation with socio-demographics and symptoms of aphrodisiac use indicated that: Male respondents, those older than 20years, the married, those from poorly educated parent and sufferers of all identified symptoms had statistically significant differences with poor perception of aphrodisiac use’ domains. This validated instrument is good for assessment of perception of aphrodisiac use among students in tertiary institution albeit with caution. A version of the scale that is broadened with highly refined items and tested for high internal validity is suggested for future studies.
Indigenous Knowledge of Enset (Ensete ventricosum (Welw.) Cheesman) Disease Protection by Gurage People, Southern Ethiopia
Authors: tesfaye henok 
Published: August 2022

Abstract

In Ethiopia, the Enset -based farming system is a major agricultural system that serves at least one-fifth of the country’s population. Farmers locally have received knowledge from generations of experience and experimentation that match neighborhood conditions. Yet, the scientific strategies were less used farmers’ Enset (Ensete ventricosum (Welw.) production and protection. This study aimed at assessing farmer’s indigenous knowledge of protecting their Enset crops form disease. The study adopted multistage sampling methods and a total of 135 respondents were selected. The study identifies, “chegino”, “Janfa”, and “quaqusa” as the major and the most important diseases that counteract the Enset production in the study area. Furthermore, the study indicates the traditional disease prevention methods of the community to control the habitually acknowledged Enset disease. Most farmers in the surveyed area use sanitation (37.8 %) or uprooting of infected Enset plants and Dis-budding (31 %) then burn or bury the residue for reducing the spread of the disease. However, crop rotation and rotation of planting holes were applied by few farmers. Besides, this study shows that the death of experienced older folks, climate variability, farmers moving to other crop production, and lack of help to apply local knowledge is the primary imperatives of the farmers to apply native knowledge on the ranchers in the study area. Thus, farmers' indigenous knowledge can be combined with scientific knowledge to improve livelihood of the farmer.
A novel auxin-inducible degron system for rapid, cell cycle-specific targeted proteolysis
Authors: Capece Marina , Tessari Anna , Mills Joseph , Vinciguerra Gian Luca Rampioni , Louke Darian , Lin Chenyu , McElwain Bryan , Miles Wayne , Coppola Vincenzo , Davies Alexander , Palmieri Dario , Croce Carlo 
Published: August 2022

Abstract
The OsTIR1/auxin-inducible degron (AID) system allows selective protein degradation upon exposure to the phytohormone auxin. However, this technology does not allow to study the effect of acute protein depletion selectively in one phase of the cell cycle. Here, we report a new AID system to Regulate OsTIR1 Levels based on the Cell Cycle Status (ROLECCS) for phase-specific target proteolysis. Finally, we applied the ROLECCS technology to show that the tumor suppressor TP53 plays a S/G2-specific role in suppression of micronuclei accumulation. This new tool allows the analysis of different protein functions during cell cycle progression with unprecedented temporal resolution.
Allocations and legal trends in the 21st century
Authors: Nelson Rebecca L. 
Published: August 2022

Abstract
Around the world, allocating water is a major task of national and sub-national water laws. Although each jurisdiction develops its laws uniquely in response to local conditions, common trends emerge across multiple jurisdictions. The 21st century has seen a geographic broadening of administrative planning and permitting or licensing arrangements for allocating traditional water sources. Allocation regimes also increasingly cover non-traditional water sources that previously fell outside their bounds, including brackish groundwater, rainwater and recycled water. Recognizing interactions between sources is of increasing concern to allocation regimes. Legal developments also provide for an increasing array of water users and other participants in allocation processes. Traditionally, allocation arrangements have centered on significant agricultural, municipal, industrial and other commercial uses. More recently, water law increasingly contemplates granting allocations to, or controlling, uses for environmental purposes and cultural purposes, and broadening access beyond current water rights holders under water market rules. Both constitutional and water laws increasingly recognize a human right to water, and increasingly inclusive processes apply to formulating water plans that guide or influence allocation regimes. These developments are not uniformly present, or present to the same degree, in all jurisdictions. However, encouragingly, they demonstrate increased recognition of the need to respond to water scarcity and give greater attention to equity and inclusion among water users and participants in allocation regimes.
Transitioning away from open access: lessons learnt from a comparative analysis of water allocation regimes worldwide
Authors: Rouillard Josselin , Babbitt Christina , Challies Edward , Rinaudo Jean-Daniel 
Published: August 2022

Abstract
The transition from open to regulated access to water resources is a challenging task for water managers who have to address complex environmental, social and economic trade-offs. Water allocation is a powerful tool, yet its implementation is deeply conflictual. This chapter compares the process of transitioning to regulated access in 13 case studies worldwide. It shows the wide diversity of institutional settings and design choices, while exploring why differences occur and considering the advantages and disadvantages of the different approaches deployed in various contexts. It concludes with key takeaways and reflections on the need for ongoing work assessing the environmental, social and economic performance of allocation regimes.
Economic performance index assessment of an industrial wastewater treatment plant in terms of the European Green Deal: effect of greenhouse gas emissions
Authors: Yapıcıoğlu Pelin , Irfan Yeşilnacar Mehmet 
Published: August 2022

Abstract
This study aims to determine the effect of greenhouse gas (GHG) emissions on economic performance in terms of energy costs for an industrial wastewater treatment plant. Also, the mitigation of GHG emissions aimed at using process modification to obtain possible reductions in energy costs. Optimum energy consumptions were reported for the minimum GHG emission using the Data Envelopment Analysis (DEA) and Monte Carlo simulation model. In this paper, a new empirical approach has been developed depending on the GHG emissions for estimating the economic performance of the wastewater treatment plants. The results revealed that nitrous oxide (N2O) emissions led to the highest energy costs among direct emissions. In the second stage of the study, the effects of design conditions on GHG emissions and energy costs were investigated. If the aeration tank is operated at 24 h of hydraulic retention time (HRT) and 22 days of solid retention time (SRT), then, on average, 27, 27.9, and 30.7% of reduction in energy costs in terms of direct carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) emissions, respectively, is observed in the plant. These reductions corresponded to approximately 17.33 €/kWh of cost-saving in this plant.
A review of the removal of heavy metal ions in wastewater by modified montmorillonite
Authors: Shi Yan , Zhong Songtao , Wang Xin , Feng Changping 
Published: August 2022

Abstract
Heavy metal pollution in water environment has always been a hot issue in environmental pollution research. Among various removal methods of heavy metal pollution, adsorption has attracted attention because of its low cost and low secondary pollution. Montmorillonite is one of an ideal adsorption materials because of its natural pore structure and a high specific surface area. In this review, the modification methods of montmorillonite are introduced. The adsorption effect of different heavy metal ions and the main factors affecting the adsorption are discussed in detail. Meanwhile, the adsorption mechanism is intensively reviewed. The removal of Cr(VI), Cd(II), Pb(II), Cu(II), Cs(I), Co(II), Hg(II), Zn(II), Ni(II), and other nine heavy metal ions by modified montmorillonite was summarized and discussed, and the optimal pH values for different adsorbents were also different, but they were all less than 7, and the removal rates of heavy metal ions by modified montmorillonite under optimal conditions were all greater than 90%. It is concluded that the composite montmorillonite material has a great adsorption potential. Finally, it is proposed that the combination of advanced oxidation and composite montmorillonite material adsorption to remove heavy metal ions is an important direction of adsorption and removal of heavy metal ions in the water environment in the future.
In silico Identification of Novel SARS-CoV-2 Main Protease and Nonstructural
Protein 13 (nsp13) Inhibitors through Consensus Docking and
Free Binding Energy Calculations
Authors: Mateev Emilio , Georgieva Maya , Zlatkov Alexander 
Published: August 2022

Background:
A new strain of a novel disease caused by severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) has been recently declared a pandemic by the World Health
Organization (WHO). The virus results in significant mortality and morbidity across the planet;
therefore, novel treatments are urgently required. Recently deposited crystallographic structures of
SARS-CoV-2 proteins have ignited the interest in virtual screenings of large databases.

Objective:
In the current study, we evaluated the inhibitory capacity of the IMPPAT phytochemical
database (8500 compounds) and the SuperDRUG2 dataset (4000 compounds) in SARS-CoV-2
main protease and helicase Nsp13 through consensus-based docking simulations.

Methods:
Glide and GOLD 5.3 were implemented in the in silico process. Further MM/GBSA calculations
of the top 10 inhibitors in each protein were carried out to investigate the binding free energy
of the complexes. An analysis of the major ligand-protein interactions was also conducted.

Results:
After the docking simulations, we acquired 10 prominent phytochemicals and 10 FDAapproved
drugs capable of inhibiting Nsp5 and Nsp13. Delphinidin 3,5,3'-triglucoside and hirsutidin
3-O-(6-O-p-coumaroyl)glucoside demonstrated the most favorable binding free energies
against Nsp5 and Nsp13, respectively.

Conclusion:
In conclusion, the analysis of the results identified that the phytochemicals demonstrated
enhanced binding capacities compared to the FDA-approved database.
Ethnopharmacology, Phytochemistry, and Pharmacology of Highland
Barley Monascus purpureus Went: A Comprehensive Review
Authors: Zhu Mei-Ning , Wu Guang , Chen Jie , Qiu Yong-Wei , Zhu Cai-Qing , Wu Yong-Zhong , Ma Qin-Ge , Zhong Guo-Yue , Wei Rong-Rui 
Published: August 2022

Background:
Highland barley Monascus purpureus Went, a traditional Tibetan medicine
with food functions, which is fermented by Monascus purpureus with highland barley as substrate.
It possesses various medical functions of promoting blood circulation and removing blood stasis,
invigorating spleen and promoting digestion in folk of the Qinghai-Tibet Plateau in China. This review
provides a comprehensive overview of ethnopharmacology, phytochemistry, and pharmacology
of highland barley Monascus purpureus Went.

Methods:
The references of highland barley Monascus purpureus Went were retrieved from the
online database, such as Web of Science, Google Scholar, SciFinder, PubMed, SpringLink, Elsevier,
Willy, CNKI, and so on.

Results:
Phytochemical research revealed that highland barley Monascus purpureus Went contained
multiple chemical components, including Monascus pigments, monacolins, lactones, and other
compounds. The reported pharmacological activities of highland barley Monascus purpureus Went
included hypolipidemic, anti-nonalcoholic fatty liver disease, and hepatoprotective activities.

Conclusions:
In a word, botany, ethnopharmacology, phytochemistry and pharmacology of highland
barley Monascus purpureus Went were reviewed comprehensively in this paper. In the future, highland
barley Monascus purpureus Went needs further study, such as paying more attention to quality
control and utilization of medicine. Therefore, this review may provide a theoretical basis and valuable
data for future studies and exploitations on highland barley Monascus purpureus Went.
Impact of Physiotherapy on Functional Status and Length of Stay of
Patients Admitted to Intensive Care Unit
Authors: Mahadevan Renukadevi , Mahesh Amrutha Hosakote , Krishnarao Chaya Sindaghatta 
Published: August 2022

Background:
Early mobilization of patients with critical illness reduces the consequences of extended periods of bed rest and improves self-care functions and quality of life. Early mobilization for mechanically ventilated patients in any ICU prevents patients from a spiral of progressive
complications, leading to either nursing home placement or persistent critical illness. Early mobilization of ICU patients has been associated with improved muscle strength and functional independence, a shorter duration of delirium, mechanical ventilation, and ICU length of stay.

Objective:
This study was undertaken to assess the impact of physiotherapy management and early
mobilization on Functional Status and Length of Stay in patients admitted to the Intensive Care
Unit.

Methods:
An observational study was conducted at a tertiary care university teaching hospital in
Mysore, South India, among patients admitted to medical and surgical intensive care units. The patients were assessed, and the patient’s baseline characteristics were recorded. The study subjects
were divided into two groups, the intervention and the control groups. There were 71 patients in the
intervention group and 67 patients control group. Subjects in the intervention group underwent
physiotherapy management and early mobilization. Subjects in the control group did not undergo
physiotherapy management and early mobilization. Physiotherapy intervention was given 2-3
times/day, and the outcome measures were the length of stay in ICU and assessment of physical
morbidity using the Chelsea Critical Care Physical Assessment Tool (CPAx).

Results:
The results showed that subjects in the intervention group were weaned from the ventilator
support (3.86±3.4 and 5.59±4.3, p = 0.005) and oxygen support (5.23±0.99 and 7.48±2.0, p = 0.000)
much earlier than the subjects in the control group. The length of ICU stay was significantly less in
the intervention group than in the control group (7.71±3.70 days and 11.64±4.8 days with a p-value,
p = 0.000). respectively

Conclusion:
This study demonstrated that implementation of physiotherapy intervention and early
mobilization in critically ill patients undergoing treatment in intensive care unit resulted in early
weaning from ventilator support and supplemental oxygen therapy, with improvement in functional
status leading to reduced length of ICU stays compared to the patients who were not given physiotherapy intervention and early mobilization.
Takotsubo Cardiomyopathy and COVID-19: A Case Report and Literature
Review
Authors: Carrizales-Sepúlveda Edgar Francisco , Flores-Ramírez Ramiro , José Solís Gabriel , Vera-Pineda Raymundo , Morales-Rendón Eliu Jefte , de Jesús Ortiz-Corona José 
Published: August 2022

Bckground:
Takotsubo cardiomyopathy is characterized by transient regional ventricular abnormalities in the absence of coronary artery disease and is reported as a complication of COVID-19.

Case Representation:
It can have a diverse clinical presentation, occasionally resembling an acute coronary syndrome, and progress to acute heart failure and cardiogenic shock with an adverse effect on patients' prognosis. A high index of suspicion and a thorough diagnostic approach supported by ancillary studies like echocardiography and coronary angiography is key for an accurate diagnosis and correct medical treatment. Herein, we report a patient with severe COVID-19 who developed Takotsubo cardiomyopathy.

Conclusion:
We also present a detailed review of the available literature regarding the relationship between COVID-19 and Takotsubo cardiomyopathy.
Current Trends, Advances, and Challenges of Tissue Engineering-Based
Approaches of Tooth Regeneration: A Review of the Literature
Authors: Hazrati Parham , Mirtaleb Mohammad Hassan , Boroojeni Helia Sadat Haeri , Koma Amir Ali Yousefi , Nokhbatolfoghahaei Hanieh 
Published: August 2022

Introduction:
Tooth loss is a significant health issue. Currently, this situation is often treated
with the use of synthetic materials such as implants and prostheses. However, these treatment modalities
do not fully meet patients' biological and mechanical needs and have limited longevity. Regenerative
medicine focuses on the restoration of patients' natural tissues via tissue engineering techniques instead of
rehabilitating with artificial appliances. Therefore, a tissue-engineered tooth regeneration strategy seems
like a promising option to treat tooth loss.

Objective:
This review aims to demonstrate recent advances in tooth regeneration strategies and discoveries
about underlying mechanisms and pathways of tooth formation

Results and Discussion:
Whole tooth regeneration, tooth root formation, and dentin-pulp organoid generation
have been achieved by using different seed cells and various materials for scaffold production. Bioactive
agents are critical elements for the induction of cells into odontoblast or ameloblast lineage. Some
substantial pathways enrolled in tooth development have been figured out, helping researchers design
their experiments more effectively and aligned with the natural process of tooth formation.

Conclusion:
According to current knowledge, tooth regeneration is possible in case of proper selection of
stem cells, appropriate design and manufacturing of a biocompatible scaffold, and meticulous application
of bioactive agents for odontogenic induction. Understanding innate odontogenesis pathways play a crucial
role in accurately planning regenerative therapeutic interventions in order to reproduce teeth.
Protective Effect of Crocin on Cadmium Induced-Overexpression of Matrix
Metalloproteinases (MMPs) Gene Family in Rat Liver
Authors: Heiat Mohammad , Varnamkhasti Farzaneh Amourizi , Heydari Seyede Fatemeh , Safari Newsha , Cheraghi Nilufar , Sohrabi Ehsan , Torabi Raheleh 
Published: August 2022

Background:
Metal pollutants such as Cadmium are known as carcinogenic agents and correlated with metastatic potential and tumor aggressiveness. Matrix metalloproteinases (MMPs) are taken into account as the focal points of metastasis. Crocin, a carotenoid chemical compound in Saffron, has been proved to have anti-cancerous properties. However, there is no reliable evidence yet to prove its anti-metastatic activity.

Objective:
The evaluation of the protective effect of Crocin against Cadmium through monitoring the expression of MMP genes.

Materials and Methods:
The in-silico analysis based on the GEO database was done for purposefully choosing MMPs. At the wet lab step, after 8 weeks of treatment of the animal (40 female Wistar rats divided into four groups of 10) with Cadmium, Crocin, complex (Cadmium+Crocin) and placebo (control), RNA extraction followed by cDNA synthesizing was done from rats’ liver tissues. Using primers for MMP genes (MMP-1, 3, 7, 12 and 13), the relative fold change of gene expression was studied. Ultimately, the statistical analysis determined the difference in gene expression between groups.

Results:
Cadmium significantly upregulated the selected MMP genes in comparison with controls. In contrast, Crocin significantly downregulated the expression of all studied MMP genes. Complex administration of Cadmium and Crocin displayed a controlled increase of expression in all MMPs except MMP-1, which was lower than the Cadmium group.

Conclusion:
These results indicated that the elevation of MMPs expression level by Cadmium was significantly suppressed by Crocin. This could promisingly demonstrate that Crocin possesses a modulating role in inhibiting overexpression of MMPs that might be considered as the anti-metastatic agent.