bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protective and risk factors of recurrent severe hypoglycemia in adults with type 1 diabetes: A cross-sectional analysis of baseline data from the PR-IAH study
Authors: Sakane Naoki , Kato Ken , Hata Sonyun , Nishimura Erika , Araki Rika , Kunichi kouyama , Hatao Masako , Matoba Yuka , Matsushita Yuichi , Domichi Masayuki , Suganuma Akiko , Sakane Seiko , Murata Takashi , Wu Fei Ling 
Published: September 2022

Abstract
Background
Severe hypoglycemia (SH) remains a central problem in the management of type 1 diabetes (T1D); however, little is known about the protective and risk factors associated with recurrent SH (RSH). This study aimed to identify the protective and risk factors for RSH in adults with T1D.
Methods
This cross-sectional study enrolled 287 adults with T1D (mean age: 50.3 ± 14.5 years, male: 36.2%, diabetes duration: 17.6 ± 11.2 years, mean HbA1c: 7.7 ± 0.9%) and divided them into non-SH (n = 262), solitary SH (n = 17), and RSH (n = 8) groups. Impaired awareness of hypoglycemia (IAH) assessed using the Gold’s method, diabetic complications, fear of hypoglycemia, diabetes distress, hypoglycemia problem solving abilities, and treatment data were collected.
Results
The overall SH rate was 16.7/100 person-years. The odds ratios of diabetic peripheral neuropathy and IAH were associated with an increased risk of RSH (odds ratio [OR]: 5.53 ,95% confidence interval [CI]: 1.18–25.98 and OR: 4.82 [95% CI:1.09–21.23], respectively); hypoglycemia problem-solving perception score was associated with a decreased risk of RSH (OR: 0.35 [95% CI: 0.18–0.69]). The rate of rtCGM use decreased as the SH category increased, but no difference was found in the rate of isCGM use.
Conclusion
We identified the protective factors, such as hypoglycemia problem-solving perception, in addition to the risk factors for RSH in adults with T1D.
Trial registration:
University hospital Medical Information Network (UMIN) Center: UMIN000039475)、Approval date 13 February 2020
LncRNA NEAT-2 Regulate the Function of Endothelial Progenitor Cells in Experimental Sepsis Model
Authors: Yin Xiao-Yi , Liu Yu , Lu Zheng-Mao , Pang Tao , Cui Hang-Tian , Xue Xu-Chao , Fang Guo-En , Luo Tian-Hang 
Published: September 2022

Abstract
Background
Sepsis is a life-threatening disease with a limited effectiveness and the potential mechanism remains unclear. LncRNA NEAT-2 plays an important role in tumor proliferation, invasion and metastasis, and also involved in the regulation of cardiovascular disease. This study aimed to investigate the function of NEAT-2 in sepsis.
Methods
We built sepsis animal model with Male Balb/C mice induced by cecal ligation and puncture (CLP). A total of 54 mice were randomly assigned into eight groups: sham operation group (n = 18), CLP group (n = 18), CLP plus si-control group (n = 3), CLP plus si-NEAT2 group (n = 3), CLP plus mimic control group (n = 3), CLP plus miR-320 group (n = 3), CLP plus normal saline group (n = 3), and normal control group (n = 3). The number of peripheral endothelial progenitor cells (EPCs), the expression level of NEAT-2 and miR-320 were detected during progression of sepsis, as well as the number of peripheral EPCs and level of TNF-α, IL-6, VEGF, ALT, AST and Cr. In addition, the function of EPCs was evaluated after NEAT-2 knockdown and miR-320 overexpression in vitro.
Results
The number of circulating EPCs increased significantly in sepsis. NEAT-2 expression was significantly increased in the progress of sepsis, accompanied with miR-320 downregulated. NEAT-2 knockdown and miR-320 overexpression attenuated hepatorenal function and increased cytokines in sepsis. Moreover, NEAT-2 knockdown and miR-320 overexpression decreased the proliferation, migration and angiogenesis of endothelial progenitor cells in vitro.
Conclusions
LncRNA-NEAT2 regulated the number and function of endothelial progenitor cells via miR-320 in sepsis.
BCRecommender System for Breast Cancer Diagnosis using Machine Learning Approaches
Authors: Bhargava Harshita , Makeri Yakubu Ajiji , Gyamenah Pius , Gupta Snehal , Vyas Geetika , Sharma Amita , Chatterjee Sreemoyee 
Published: September 2022

Abstract
Background: Early detection of breast cancer is challenging and necessitates more thorough observation. Researchers and medical professionals are always looking for ways to detect breast cancer early, systematically, and affordably. Other studies on cancer have developed a range of recommendation systems, such as those for food and medicine, disease prediction based on text and image data, and prognosis. The only drawback to such systems is that they are highly specialized and only accept specific types of data. To be more precise in diagnosis, we need systems that consider all aspects of the disease and provide continuous support at all the stages of breast cancer.
Methods: A recommendation model called "BCRecommender" has been put forth to diagnose and comprehend breast cancer using clinical and histopathological slides while taking feature-based classification into consideration. It has a layered architecture that accepts different reports (structured and unstructured data), and it produces results differently with respect to each layer. It is effective for cancer in all stages, from early to advanced.Existing breast cancer repositories are used to design a system.
Results & Conclusions:The model performance varies at different layers.In layer1 the bagging classifier achieves the highest accuracy of 61.06% while in layer2 bagging has the highest accuracy of 97.52%. Layer 3 has an accuracy of 97.39% after augmentation. During the test phase, confidence ranges from 60%-100%.In Layer 4 confidence varies from 50%-100%.
Topological superconductivity in a topological insulator
Authors: Chen Xiao-Jia , Yu Hao , Yuan Noah , Li Wei-Jian , Chen Liu-Cheng , Cao Zi-Yu , Li Di , Qin Xiaoying , Gong Chang-De 
Published: September 2022

Abstract
Topological superconductivity as an exotic quantum phenomenon with coupled nontrivial topological order and superconductivity together in a single substance has drawn extensive attention because of its novelty as well as the potential for quantum computation1-6. A direct idea for producing topological superconductors is to create superconductivity based on the well recognized topological insulators7-14. The topological insulating states in highly efficient thermoelectric materials Bi2Te3 and Bi2Se3 and their alloy B2Te3-xSex have been established from angle-resolved photoemission15-17 and transport18 experiments. Superconductivity was also observed based on these popular topological insulators by the application of pressure19-21, chemical dopant22, and heterostructures23,24. However, the experiments mainly focusing on Bi$_{2}$Se$_3$ doped by metals such as Cu25-35, Sr36-39, and Nb40-46 have not provided the consistent evidence to support the topological superconductivity. Here we carry out a systematic high-pressure study on a topological insulator Bi2Te2.7Se0.3 to provide the convincing evidence for the expected topological superconductivity. Four phases with different structures are found upon compression by the X-ray diffraction, Raman scattering, and electrical transport measurements. The Hall resistivity and electronic structure calculations help to identify the topological surface state in the entire initial phase, while superconductivity is found to coexist with such a state of the compressed material after its passing the electronic topological transition, followed by three other superconducting phases without topological character. For these superconducting phases, we observe that the upper critical field follows with the temperature in the critical exponent 2/3 for the first one with the topological surface state and 1 for the left. These observations support the realization of the topological superconductivity in the initial phase according to the theoretically proposed critical field measure. Therefore, this work points out a big pool and new direction for finding topological superconductors from topological thermoelectric materials. The results also demonstrate that the critical field measure is an easy and powerful method for the identification of topological superconductivity in the system where topological surface states and superconductivity coexist.
Long loop technique with bifemoral access as salvage technique for repositioning of dislodged port catheters
Authors: Bosch Vincent Van den , De Beukelaer Frédéric , Isfort Peter , Keil Sebastian , Kuhl Christiane K. , Bruners Philipp , Pedersoli Federico 
Published: September 2022

Abstract
Background
Repositioning of dislocated port systems’ catheters is usually performed with a pigtail catheter and/or a goose snare. In case of an inaccessible port catheter tip due to thrombosis, this classic approach may be not successful. For these cases, we describe a long loop bailout technique with bifemoral access.
Technique
Via a right transfemoral access, a first attempt to reposition the dislodged port catheter using pigtail catheter and goose snare was performed. After an unsuccessful attempt and delineation of thrombosis of the catheter tip, the contralateral femoral vein was subsequently punctured and a sheath was placed. Through both vascular sheaths, pigtail catheter and goose wire were advanced distally to the catheter. The guidewire in the pigtail catheter was snared, thus creating a “Long loop” configuration. Pulling down both catheters simultaneously with improved stability allowed to detach the catheter tip from the vessel wall and replacement into the superior vena cava was possible. Refinement of catheter tip position was done using the goose snare . This technique was applied on 5 patients with dislodged port catheters in the jugular vein (2/5), the innominate vein (1/5), the subclavian vein (1/5) and the azygos vein (1/5) with a technical success of 100%. No complications were observed.
Conclusion
The Long loop technique can be used as salvage approach to reposition a dislodged catheter in case of failure with pigtail catheter and goose snare.
Prevalence of Intestinal and Soil Transmitted Helminths Among Primary School Children of Badessa District, West Hararghe Zone, Oromia Region, Eastern Ethiopia
Authors: Wolde Amsalu Mekonnen 
Published: September 2022

Abstract
Background: Helminthes are known as parasitic worms (1). Helminthes of medically important are belonging to two phyla: the Platy helminths as flat worms and Nematy helminths or round worms. Platy helminths are further subdivided into Cestodes and Trematodes (2). According to some literatures, parasitic worms are categorized into three groups: Cestodes, nematodes, and Trematodes (1). In this study, the prevalence of intestinal and soil transmitted helminths among primary school children were assessed by two stool examination methods namely: wet mount and Kato-Katz thick smear techniques. for the diagnosis of intestinal helminths were compared to detect human intestinal helminths.
Objective: To assess the prevalence of intestinal and soil transmitted helminths among primary school children in Badessa woreda, west Hararghe zone, Oromia region, eastern Ethiopia.
Methodology: A cross-sectional study design was conducted for the study then four primary schools were selected by lottery method from 11 primary schools. Finally, 200 primary school children had given a stool specimen for wet mount and Kato-Katz methods so that two types of stool specimens were collected from school children aged 6 to 16 from four primary schools randomly selected in Badessa woreda, West Hararghe Zone, Eastern Ethiopia. Each specimen was smeared on one slide for every technique with normal saline for wet mount and a cellophane cover for Kato-Katz technique. The overall prevalence of the parasites in each school of the woreda was determined.
Result: The prevalence of Ascaris lumbricoides (19.5%), Eterobius vermicularis (13%) and Hookworm (49%) was high in schools 1, 2 and 4. The prevalence of Schistosoma mansoni (38%) high in school 3 (Table 3). Finally, Kato-Katz technique had played more role and was more effective and sensitive than the stool wet mount technique in order to find the result.
Conclusion: Intestinal and soil transmitted helminths were significantly detected especially from carriers and non-symptomatic children. Kato-Katz technique was more selective and sensitive than wet mount method.
Paradoxical effect of the antipsychotic drug sulpiride in the ventral pallidum: impairs learning and induces place preference.
Authors: Dusa Daniella , Ollmann Tamas , Kallai Veronika , Lenard Laszlo , Kertes Erika , Berta Beata , Szabo Adam , Laszlo Kristof , Galosi Rita , Zagoracz Olga , Karadi Zoltan , Peczely Laszlo 
Published: September 2022

AbstractSulpiride, as a D2-like dopamine (DA) receptor (D2R) antagonist, is an important antipsychotic drug in the treatment of schizophrenia. Recently, we have shown that the activation of D2Rs in the ventral pallidum (VP) modulates the activity of the ventral tegmental area (VTA) DAergic neurons. According to our hypothesis, intra-VP sulpiride can influence the motivational and learning processes, pervasively modifying the behavior of examined animals. In the present study, sulpiride was microinjected into the VP of male Wistar rats in three different doses. Morris water maze (MWM) test was applied to investigate the effects of sulpiride on spatial learning, while conditioned place preference (CPP) test was used to examine the potential rewarding effect of the drug. In order to show, whether the animals can associate the rewarding effect with an area which can be recognized only on its spatial location, we introduced a modified version of the CPP paradigm, the spatial CPP test. Our results show that the intra-VP sulpiride dose-dependently impairs learning processes. However, the largest dose of sulpiride induces place preference. Results of the spatial CPP paradigm demonstrate that the animals cannot associate the rewarding effect of the drug with the conditioning area based on its spatial location. In the CPP paradigm, likely faster habituation with the conditioning environment could be observed in the sulpiride-treated rats. In summary, we can conclude that intra-VP sulpiride has a dual effect: it diminishes the hippocampus-dependent spatial learning processes, in addition, it has a dose-dependent rewarding effect.
Good-Bootstrap: Simultaneous Confidence Intervals for Large Alphabet Distributions
Authors: Marton Daniel , Painsky Amichai 
Published: September 2022

Abstract
Simultaneous confidence intervals (SCI) for multinomial proportions are a corner stone in count data analysis and a key component in many applications. A variety of schemes were introduced over the years, mostly focusing on an asymptotic regime (where the sample is large), or a small sample regime, where the alphabet size is relatively small. In this work we introduce a new SCI framework which considers a large alphabet setup. Our proposed framework utilizes bootstrap sampling with the Good-Turing probability estimator as a plug-in distribution. We demonstrate the favorable performance of our proposed method in synthetic and real-world experiments. Importantly, we provide an exact analytical expression for the bootstraped statistic, which replaces the computationally costly sampling routine. Our proposed framework is publicly available at the first author's webpage.
ABCB1 regulates myeloid-derived suppressor cells-related immune factors in breast cancer
Authors: Chen Han-Kun , Chen Yi-Ling , Wang Chih-Yang , Chung Wei-Pang , Fang Jung-Hua , Lai Ming-Derg , Hsu Hui-Ping 
Published: September 2022

Abstract
Purpose Resistance to standard chemotherapy is a critical problem for breast cancer patients. The ATP-binding cassette (ABC) superfamily transporters actively pump out drugs and play an important role in chemoresistance. ABCB1 (ABC subfamily B, member 1, also named as multidrug resistance protein 1, MDR1) and suppressive myeloid-derived suppressor cells (MDSCs) potentially involve in chemoresistance of breast cancer. The relationship between ABCB1 and MDSC is less studied. Methods Microarray or RNA sequencing data was obtained from The Cancer Genome Atlas Breast Invasive Carcinoma in Genomic Data Commons Data Portal (GDC TCGA-BRCA) and GEO database. Expression of ABCB1 and MDSC-related genes was compared. Patient-derived xenograft (PDX) from HER2-enriced breast cancer was established to investigate the association of ABCB1 and MDSC-related genes in breast cancer. Results Expression of ABCB1 was increased in doxorubicin-selected MCF-7/ADR cells. High expression of ABCB1 mRNA was correlated with lymph node metastasis and worse overall survival of breast cancer patients. ABCB1 was positively correlated with IL6, CSF1, CSF3, or PTGS2 and negatively correlated with VEGF. PDX model from HER2-enriched stage IIA breast cancer was established. Treatment with doxorubicin or paclitaxel suppressed growth of P2 tumors and expression of ABCB1. Expression of IL6, CSF1, CSF3, PTGS2 was suppressed by paclitaxel, but not by doxorubicin. Intrasplenic MDSCs, including CD11b+Ly6G+ and CD11b+Ly6C+ cells, were higher than intratumor MDSCs in PDX-carrying nude mice. Clinically, the patient developed cancer recurrence after adjuvant chemotherapy with doxorubicin-based regimen and was well-controlled after paclitaxel-trastuzumab combined therapy.Conclusions ABCB1 is a poor predictor of breast cancer patients. Regulation of MDSC-related immune factors by ABCB1 and immune response to chemotherapeutic agents also contributes to cancer recurrence and treatment effect. PDX model is suitable to test expression of targeting genes and potential interaction with immune cells.
Histopathological Image Analysis Using Deep Learning Framework
Authors: RANI SUDHA , M JOGENDRA KUMAR 
Published: September 2022

AbstractBreast cancer has the highest morbidity and fatality rates of all cancers. Early identification of this is critical step in histopathological image analysis (HIA). Manual methods are will take much time and also having of lot errors in pathologists' competence. Current HIA, ignoring on histopathology image segmentation of breast cancer (BC) because to its complicated features and unavailability of previous data with detailed annotations. Our approach uses only graph-based segmentation to classify breast cancer histopathology images. Graph based segmentation images extract efficient features. Then, using recursive feature elimination (RFE), images of breast cancer are categorized. The solution provided here tackles large-scale image processing in breast cancer histopathology images. The suggested technique accurately classifies breast histopathology pictures as abnormal or normal, supporting early breast cancer diagnosis.
Clinical features of metabolism-related fatty liver disease in the non-lean population
Authors: Li Minran , Li Jin-Zhong , Wang Cun-chuan , Yuan Rui-kun , Ye Li-hong , Liu Yun-yan , Liang Xu-jing , Zhang Hai-cong , Liu Zhi-quan , Zeng Dong-yu , Zhang Xue-dong , Wang De-hua , Li Jun-qing , Li Tao-yuan , Yang Liu , Li Jie-ying , Cao Yang , Pan Yun , Lin Xun-ge , Pan Calvin Q. , Dai Er-hei , Dong Zhi-yong 
Published: September 2022

Abstract
Objective
To assess the clinical and histological features of metabolic associated fatty liver disease (MAFLD) in non-lean population.
Methods
Current study enrolled consecutive non-lean (Body Mass Index (BMI) > 23 kg/m2) patients with MAFLD and available liver biopsy results. Patients were stratified by BMI into two groups for the comparison of their clinical and histological variables, which included the overweight (BMI 23 ~ < 28 kg/m2) and the obese (BMI ≥ 28 kg/m2). Risk factors for moderate to severe fibrosis (stage > 1) were also analysed through the logistic regression model.
Results
Among 184 non-lean patients with MALFD enrolled, 65 and 119 were overweight and obese, respectively. Patients in the obesity group had a significantly lower level of gamma-Glutamyl transpeptidase (GGT), higher levels of platelet (PLT), Glucose (Glu), prothrombin time (PT), and more common of moderate to severe inflammatory activity when compared to those in the overweight group. However, a significant low frequency of moderate to severe fibrosis was found in the obesity group vs the overweight group (19.33% vs 40.00%, P = 0.002). Multivariate logistic regression analysis of fibrosis found that aspartate transaminase (AST), BMI, alanine transaminase (ALT) and cholesterol (CHOL) were independent predictors for moderate to severe fibrosis in non-lean patients with MAFLD. Compared with the traditional FIB-4 (AUC = 0.77) and APRI (AUC = 0.79) indexes, the combined index based on AST, BMI, ALT and CHOL was more accurated in predicting moderate to severe fibrosis in non-lean patients with MAFLD (AUC = 0.87).
Conclusions
Clinical and histological features differed between obesity and overweight patients with MAFLD. When compared to the traditional serum markers, the combination index including AST, BMI, ALT and CHOL provides a better model to predictor moderate to severe fibrosis in non-lean patients with MAFLD.
Clinical and pathological analysis of intestinal metaplasia and intraepithelial neoplasia of gastric mucosa: intestinal metaplasia inhibits the progression of intraepithelial neoplasia
Authors: Gao Rui , Zhang Xi , Chen Xiaoyan , Chen Xin , Pan Danling , Jin Long , Lin Ying , Zheng Huawei , Yu Xunbin , Chen Xin(F) 
Published: September 2022

Abstract
Background
At present, whether intestinal metaplasia of the gastric mucosa is related to gastric cancer is still controversial. Many research results showed that intestinal metaplasia was a risk factor for gastric cancer, while some scholars believe that intestinal metaplasia is not related to precancerous lesions. Through clinicopathological analysis, we found that intestinal metaplasia of the gastric mucosa inhibits the progression of intraepithelial neoplasia.
Methods
We collected the clinicopathological data of patients with gastric mucosa biopsy, and analyzed the relationship between intestinal metaplasia of gastric mucosa and neutrophil or intraepithelial neoplasia by histology, immunohistochemistry and immunohistochemistry.
Results
In patients with intestinal metaplasia of the gastric mucosa, the proportion of patients with intraepithelial neoplasia was 9.8%, while in patients with intraepithelial neoplasia, the proportion of patients with intestinal metaplasia was as high as 73.8%. The number of glands with intestinal metaplasia infiltrated by neutrophils was significantly less than that in non intestinal metaplasia glands infiltrated by neutrophils. In the glands with intestinal metaplasia and intraepithelial neoplasia, the expression index of Ki67 was significantly lower than that in intraepithelial neoplasia without intestinal metaplasia; and in the glands with intraepithelial neoplasia and intestinal metaplasia, the Ki67 expression index decreased with the increasing proportion of goblet cells. The expression of p53 in glands with intraepithelial neoplasia was high or lost, but was normal in glands with intestinal metaplasia.
Conclusions
Cells with intraepithelial neoplasia may originate from non goblet cells of intestinal metaplasia. Intestinal metaplasia plays a protective role to glands and inhibits intraepithelial neoplasia.