bluuebunny/crossref_metadata_2025_split huggingface dataset:
Importância da produção de mudas para a arborização urbana: viveiros públicos de Teresina, Piauí, Brazil
Authors: Moraes Lorran André , Passos Tainara Lima de Sousa , Santos Leilson Alves dos , Lima Adriana de Sousa , Batista Waldiléia Ferreira de Melo , Santos Kelly Polyana Pereira , Machado Roselis Ribeiro Barbosa 
Published: February 2022

Em face da urbanização, a sociedade tem sido afetada no que diz respeito à qualidade de vida e ao bem-estar. Preservar e integrar espécies vegetais no ambiente urbano tem sido sinônimo de sobrevivência. Dessa forma, os viveiros de mudas se apresentam como elemento essencial para a arborização urbana. Neste sentido, o presente trabalho apresenta informações de dois viveiros de mudas da cidade de Teresina, capital do Piauí, apresentando um diagnóstico acerca de suas dinâmicas, compreendendo como são realizadas cada etapa e fases do processo de coleta, armazenamento e beneficiamento das sementes, produção, manejo, controle, identificação e distribuição das mudas. Apresenta-se, ainda, dados sobre a rotina de produção de mudas e os processos que envolvem desde a coleta das sementes a sua distribuição à população. A abordagem técnica da pesquisa é qualitativa, embasada na pesquisa bibliográfica, de campo e a documental. Nessa pesquisa, observou-se que são produzidas nos viveiros mudas de aproximadamente 80 espécies botânicas. Os resultados demonstram a importância desses locais possuem para a população, a Secretaria de Meio Ambiente e para a comunidade cientifica, sendo ainda, de grande relevância para o planejamento futuro. Portanto, arborizar vai além de plantar mudas em vários lugares, requer planejamento bem gerenciado, assim o conhecimento da dinâmica em viveiros de mudas bem como o repasse dessas informações gera condições de segurança na qualidade e no bem-estar das futuras gerações.
Antibacterial activity of Kombucha against Escherichia coli and Staphylococcus aureus and Minas Frescal cheese production with Scoby
Authors: Coelho Eduardo Bergamaski , Saraiva Bruna Barnei , Rodrigues Bruna Moura , Silva Júnior Ranulfo Combuca da , Campanholi Katieli da Silva Souza , Maricato Dayse Maria Bernardo , Pintro Paula Matumoto , Pozza Magali Soares dos Santos 
Published: January 2022

Functional ingredients have become common in food products. The fermented Kombucha drink stands out for its probiotic and antimicrobial properties that beneficially contribute to food use. The present study aimed at assessing the antimicrobial activity of Kombucha in experimentally contaminated cheeses. Therefore, Minas Frescal cheeses were prepared, inoculated with Escherichia coli and divided into three treatments: 1) lactic acid bacteria (BioRich®); 2) lactic bacteria (BioRich ®) and 10 g of Scoby (symbiotic culture of bacteria and yeasts); 3) 10 g of Scoby. The gelatinous film (Scoby) was used to produce the Kombucha extract. The experimental design was a 3 x 4 factorial arrangement with three treatments and four evaluation times. Data was analyzed with ANOVA, and treatments means were compared with the Tukey test (p £ 0.05). The parameters of minimum inhibitory concentration, color, texture, pH, acidity, water activity, counting of yeasts, lactobacilli, and total coliforms were evaluated on days 0, 3, 6, and 9 of storage. Antibacterial action of Kombucha was compared against Escherichia coli and Staphylococcus aureus bacteria for minimum inhibitory concentration. Acidity, bacterial counting, yeasts, coliforms, lactobacilli, hardness, and gumminess showed significant changes with the addition of Kombucha to cheeses (p £ 0.05). However, However, the addition of Kombucha to the cheeses did not reduce the coliform content and negatively altered the texture parameters.
Avaliação do nível de conhecimento da prescrição de AINEs para equinos no nordeste brasileiro
Authors: Santos Júnior Dinamérico de Alencar , Carvalho Caio Victor Damasceno , Bomfim Felipe Pires de Souza , Pereira Matheus Teixeira Borges , Carvalho Saulo de Almeida , Miranda Neto Eldinê Gomes de , Escodro Pierre Barnabé 
Published: February 2022

No presente estudo foram aplicados 100 questionários (50 por grupo) com o objetivo de identificar quais os principais AINEs recomendados por médicos veterinários (G1) e vendedores/balconistas de lojas agropecuárias (G2) na região Nordeste do Brasil para o tratamento das afecções dos sistemas locomotor e digestório da espécie equina. Para as afecções do sistema locomotor: como primeira opção, a fenilbutazona foi descrita como principal AINE nos dois grupos (G1: 58% e G2: 28%). Como segunda opção, o flunixin meglumine foi o mais recomendado (G1: 32% e G2: 24%). Como terceira opção, o meloxicam foi o mais recomendado também por ambos (G1:26% e G2: 16%). Para as afecções do sistema digestório: como primeira opção, o flunixin meglumine foi o fármaco mais recomendado (G1: 54% e G2: 40%). Como segunda opção, para o G1, flunixin meglumine (36%) e para o G2, a dipirona (30%). Como terceira opção, para o G1, o meloxicam e o DMSO foram os mais citados (18% cada) e para o G2, a fenilbutazona (18%). Os AINEs não seletivos são os mais recomendados por veterinários e vendedores de lojas agropecuárias do Nordeste do Brasil para tratamento das afecções locomotoras e digestivas em equinos, enquanto que os inibidores seletivos para COX-2 ainda são pouco recomendados. O desconhecimento das dosagens técnicas foi constatado em ambos os grupos, com destaque para o G2. Medidas de controle na venda de AINEs e orientação sobre seu uso correto aos profissionais do cavalo fazem-se necessárias.
Imunocards: aprendendo as defesas do organismo jogando
Authors: Vale Filho Maurício de Almeida , Santos Pablo de Castro , Maia Allyssandra Maria Lima Rodrigues 
Published: February 2022

O estudo da Imunologia revela a importância de se conhecer a atuação do Sistema Imunológico nas defesas do organismo e sua habilidade de resposta à ação de substâncias imuno estimulantes e/ou invasão de microrganismos do ambiente. Esse conteúdo deve ser ministrado na disciplina de Biologia, sobretudo por entender-se, que na educação básica, a formação conceitual e o estabelecimento de relações entre estes conceitos são primordiais para a formação do indivíduo. Nessa perspectiva, o trabalho teve como objetivo desenvolver um jogo de cartas do tipo baralho, de caráter lúdico, educativo e investigativo e inseri-lo no contexto do ensino da Imunologia para alunos do ensino médio, de uma escola estadual do município de Russas-CE. Metodologias diversificadas como o uso de jogos tem elevada aceitação dentre os estudantes, e estes, mediante a atividade, demonstram melhor compreensão global dos temas tratados na disciplina. A pesquisa foi realizada com base no método interpretativo em consonância com a abordagem quali-quantitativa. A análise interpretativa dos dados qualitativos revelaram uma boa percepção dos estudantes acerca do tema proposto, logo, os mesmos apresentaram conhecimentos prévios importantes para uma boa compreensão do conteúdo nas aulas de Biologia. Os resultados quantitativos por sua vez evidenciaram que o jogo IMUNOCARDS auxiliou na compreensão do estudo da Imunologia, bem como, facilitou a aquisição de informações e a produção de novos conhecimentos. Os dados permitiram ainda, perceber que o jogo se mostrou uma ferramenta criativa, agradável, atraente, lúdica e inovadora no ensino de Biologia.
Maternal Low-Protein Diet Deregulates DNA Repair and DNA Replication Pathways in Female Offspring Mammary Gland Leading to Increased Chemically Induced Rat Carcinogenesis in Adulthood
Authors: Zapaterini Joyce R. , Fonseca Antonio R. B. , Bidinotto Lucas T. , Colombelli Ketlin T. , Rossi André L. D. , Kass Laura , Justulin Luis A. , Barbisan Luis F. 
Published: February 2022

Studies have shown that maternal malnutrition, especially a low-protein diet (LPD), plays a key role in the developmental mechanisms underlying mammary cancer programming in female offspring. However, the molecular pathways associated with this higher susceptibility are still poorly understood. Thus, this study investigated the adverse effects of gestational and lactational low protein intake on gene expression of key pathways involved in mammary tumor initiation after a single dose of N-methyl-N-nitrosourea (MNU) in female offspring rats. Pregnant Sprague–Dawley rats were fed a normal-protein diet (NPD) (17% protein) or LPD (6% protein) from gestational day 1 to postnatal day (PND) 21. After weaning (PND 21), female offspring (n = 5, each diet) were euthanized for histological analysis or received NPD (n = 56 each diet). At PND 28 or 35, female offspring received a single dose of MNU (25 mg/kg body weight) (n = 28 each diet/timepoint). After 24 h, some females (n = 10 each diet/timepoint) were euthanized for histological, immunohistochemical, and molecular analyses at PDN 29 or 36. The remaining animals (n = 18 each diet/timepoint) were euthanized when tumors reached ≥2 cm or at PND 250. Besides the mammary gland development delay observed in LPD 21 and 28 groups, the gene expression profile demonstrated that maternal LPD deregulated 21 genes related to DNA repair and DNA replication pathways in the mammary gland of LPD 35 group after MNU. We further confirmed an increased γ-H2AX (DNA damage biomarker) and in ER-α immunoreactivity in mammary epithelial cells in the LPD group at PND 36. Furthermore, these early postnatal events were followed by significantly higher mammary carcinogenesis susceptibility in offspring at adulthood. Thus, the results indicate that maternal LPD influenced the programming of chemically induced mammary carcinogenesis in female offspring through increase in DNA damage and deregulation of DNA repair and DNA replication pathways. Also, Cidea upregulation gene in the LPD 35 group may suggest that maternal LPD could deregulate genes possibly leading to increased risk of mammary cancer development and/or poor prognosis. These findings increase the body of evidence of early-transcriptional mammary gland changes influenced by maternal LPD, resulting in differential response to breast tumor initiation and susceptibility and may raise discussions about lifelong prevention of breast cancer risk.
Visual Function is Gradually Restored During Retina Regeneration in Adult Zebrafish
Authors: Hammer Juliane , Röppenack Paul , Yousuf Sarah , Schnabel Christian , Weber Anke , Zöller Daniela , Koch Edmund , Hans Stefan , Brand Michael 
Published: February 2022

In comparison to mammals, zebrafish are able to regenerate many organs and tissues, including the central nervous system (CNS). Within the CNS-derived neural retina, light lesions result in a loss of photoreceptors and the subsequent activation of Müller glia, the retinal stem cells. Müller glia-derived progenitors differentiate and eventually restore the anatomical tissue architecture within 4 weeks. However, little is known about how light lesions impair vision functionally, as well as how and to what extent visual function is restored during the course of regeneration, in particular in adult animals. Here, we applied quantitative behavioral assays to assess restoration of visual function during homeostasis and regeneration in adult zebrafish. We developed a novel vision-dependent social preference test, and show that vision is massively impaired early after lesion, but is restored to pre-lesion levels within 7 days after lesion. Furthermore, we employed a quantitative optokinetic response assay with different degrees of difficulty, similar to vision tests in humans. We found that vision for easy conditions with high contrast and low level of detail, as well as color vision, was restored around 7–10 days post lesion. Vision under more demanding conditions, with low contrast and high level of detail, was regained only later from 14 days post lesion onwards. Taken together, we conclude that vision based on contrast sensitivity, spatial resolution and the perception of colors is restored after light lesion in adult zebrafish in a gradual manner.
Assessing Autosomal InDel Loci With Multiple Insertions or Deletions of Random DNA Sequences in Human Genome
Authors: Yao Yining , Sun Kuan , Yang Qinrui , Zhou Zhihan , Shao Chengchen , Qian Xiaoqin , Tang Qiqun , Xie Jianhui 
Published: February 2022

Multiple mutational events of insertion/deletion occurring at or around InDel sites could form multi-allelic InDels and multi-InDels (abbreviated as MM-InDels), while InDels with random DNA sequences could imply a unique mutation event at these loci. In this study, preliminary investigation of MM-InDels with random sequences was conducted using high-throughput phased data from the 1000 Genomes Project. A total of 3,599 multi-allelic InDels and 6,375 multi-InDels were filtered with multiple alleles. A vast majority of the obtained MM-InDels (85.59%) presented 3 alleles, which implies that only one secondary insertion or deletion mutation event occurred at these loci. The more frequent presence of two adjacent InDel loci was observed within 20 bp. MM-InDels with random sequences presented an uneven distribution across the genome and showed a correlation with InDels, SNPs, recombination rate, and GC content. The average allelic frequencies and prevalence of multi-allelic InDels and multi-InDels presented similar distribution patterns in different populations. Altogether, MM-InDels with random sequences can provide useful information for population resolution.
Topographical Relationship Between Acute Macular Neuroretinopathy and Choroidal Watershed Zone or Patchy Choroidal Filling
Authors: Duan Jialiang , An Jianbin , Li Minhao , Zhang Zhengwei , Zhou Liang , Yin Pengfei , Ma Jingxue , Shang Qingli 
Published: February 2022

PurposeTo study the topographical relationship between acute macular neuroretinopathy (AMN) lesions and the choroidal watershed zone (CWZ) or patchy choroidal filling (PCF) using multimodal imaging.MethodsLesions in patients diagnosed with AMN were clinically examined using multimodal imaging, including fundus photography, near-infrared reflectance imaging, spectral-domain optical coherence tomography (OCT), fluorescein angiography, indocyanine green angiography, OCT angiography, and microperimetry. The topographical relationship between AMN and the CWZ or PCF was evaluated.ResultsSeven eyes of six patients were included in the study. The mean age of the patients was 35.8 ± 11.7 years. The AMN lesions were collocated with the CWZ in five eyes and the PCF in one eye. Among these eyes, three had complete patterns, and three had partial patterns. Only one eye showed no topographical relationship between AMN and the CWZ or PCF.ConclusionThe colocation of AMN and CWZ/PCF suggests that the AMN lesions were within an area with a dual-watershed zone: the watershed zone between the retinal deep capillary plexus and choriocapillaris, and the choroidal watershed zone or patchy choroidal filling. This retinal area was highly vulnerable to hypoperfusion. Our results suggest a novel pathophysiological mechanism for AMN.
Association Between Frailty and Inpatient Services Utilization Among Older Adults in Rural China: The Mediating Role of Multimorbidity
Authors: Yuan Yemin , Li Jie , Fu Peipei , Zhou Chengchao , Li Shixue 
Published: February 2022

IntroductionDeveloped and developing countries have different health systems and disease patterns. There is little evidence that frailty is related to inpatient services utilization in developing countries. In addition, the underlying mechanism of this relationship also remains unclear. This study aimed to examine the association between frailty and inpatient services utilization, and further explore whether multimorbidity play a mediating role in this association.MethodsA total of 3,242 rural older adults aged 60 and older were included in the analysis. Frailty was measured by the physical frailty phenotype (PFP). Multimorbidity and inpatient services utilization was measured based on participants' self-report and validated by village doctors. Ordered logistic regression analyses were performed to examine the association between frailty, multimorbidity and inpatient services utilization. Bootstrap analysis was further to explore the mediation effect of multimorbidity on frailty and inpatient services utilization.ResultsThe utilization of inpatient services was 20.1% (one: 15.8%, two or more: 4.3%). The prevalence of prefrailty and frailty was 64.7 and 18.1%, respectively. Frail older adults experienced a higher risk of multimorbidity and inpatient services utilization. Multimorbidity partially mediated the association between frailty and inpatient services utilization [95% confidence interval (CI): 0.005-0.016, p &lt; 0.001]. The mediating effect of multimorbidity accounted for 19.0% of the total effect.ConclusionsAmong Chinese rural older adults, frailty is associated with higher inpatient services utilization, and multimorbidity mediates this association. Recommendations are to increase frailty risk screening, chronic disease monitoring, and to do timely interventions.
Immigrant IBD Patients in Spain Are Younger, Have More Extraintestinal Manifestations and Use More Biologics Than Native Patients
Authors: Gutiérrez Ana , Zapater Pedro , Ricart Elena , González-Vivó María , Gordillo Jordi , Olivares David , Vera Isabel , Mañosa Míriam , Gisbert Javier P. , Aguas Mariam , Sánchez-Rodríguez Eugenia , Bosca-Watts Maia , Laredo Viviana , Camps Blau , Marín-Jiménez Ignacio , Zabana Yamile , Martín-Arranz María Dolores , Muñoz Roser , Navarro Mercè , Sierra Eva , Madero Lucía , Vela Milagros , Pérez-Calle José Lázaro , Sainz Empar , Calvet Xavier , Arias Lara , Morales Victor , Bermejo Fernando , Fernández-Salazar Luis , Van Domselaar Manuel , De Castro Luisa , Rodríguez Cristina , Muñoz-Villafranca Carmen , Lorente Rufo , Rivero Montserrat , Iglesias Eva , Herreros Belén , Busquets David , Riera Joan , Martínez-Montiel María Pilar , Roldón Marta , Roncero Oscar , Hinojosa Esther , Sierra Mónica , Barrio Jesús , De Francisco Ruth , Huguet José , Merino Olga , Carpio Daniel , Ginard Daniel , Muñoz Fernando , Piqueras Marta , Almela Pedro , Argüelles-Arias Federico , Alcaín Guillermo , Bujanda Luis , Manceñido Noemí , Lucendo Alfredo J. , Varela Pilar , Rodríguez-Lago Iago , Ramos Laura , Sempere Laura , Sesé Eva , Barreiro-de Acosta Manuel , Domènech Eugeni , Francés Rubén 
Published: February 2022

BackgroundPrevious studies comparing immigrant ethnic groups and native patients with IBD have yielded clinical and phenotypic differences. To date, no study has focused on the immigrant IBD population in Spain.MethodsProspective, observational, multicenter study comparing cohorts of IBD patients from ENEIDA-registry who were born outside Spain with a cohort of native patients.ResultsWe included 13,524 patients (1,864 immigrant and 11,660 native). The immigrants were younger (45 ± 12 vs. 54 ± 16 years, p &lt; 0.001), had been diagnosed younger (31 ± 12 vs. 36 ± 15 years, p &lt; 0.001), and had a shorter disease duration (14 ± 7 vs. 18 ± 8 years, p &lt; 0.001) than native patients. Family history of IBD (9 vs. 14%, p &lt; 0.001) and smoking (30 vs. 40%, p &lt; 0.001) were more frequent among native patients. The most prevalent ethnic groups among immigrants were Caucasian (41.5%), followed by Latin American (30.8%), Arab (18.3%), and Asian (6.7%). Extraintestinal manifestations, mainly musculoskeletal affections, were more frequent in immigrants (19 vs. 11%, p &lt; 0.001). Use of biologics, mainly anti-TNF, was greater in immigrants (36 vs. 29%, p &lt; 0.001). The risk of having extraintestinal manifestations [OR: 2.23 (1.92–2.58, p &lt; 0.001)] and using biologics [OR: 1.13 (1.0–1.26, p = 0.042)] was independently associated with immigrant status in the multivariate analyses.ConclusionsCompared with native-born patients, first-generation-immigrant IBD patients in Spain were younger at disease onset and showed an increased risk of having extraintestinal manifestations and using biologics. Our study suggests a featured phenotype of immigrant IBD patients in Spain, and constitutes a new landmark in the epidemiological characterization of immigrant IBD populations in Southern Europe.
Associations of the Dietary Iron, Copper, and Selenium Level With Metabolic Syndrome: A Meta-Analysis of Observational Studies
Authors: Ding Jun , Liu Qi , Liu Ze , Guo Hongbin , Liang Jieyu , Zhang Yi 
Published: February 2022

BackgroundEpidemiological studies have investigated the associations of dietary iron, copper, and selenium level with metabolic syndrome (MetS). However, their results are conflicting. This meta-analysis of observational study was, therefore, employed to investigate the associations above.MethodsA comprehensive literature search was employed using PubMed, Web of Science, Embase, and Scopus database up to October 2021 (no restriction was set for the initiate time). The pooled relative risk (RR) of MetS for the highest vs. lowest dietary iron, copper, and selenium level was estimated, respectively.ResultsA total of 14 observational studies (55,131 participants) were identified as meeting the inclusion criteria. Specifically, 7 studies were related to the dietary iron level. The overall multivariable adjusted RR demonstrated that the dietary iron level was positively associated with MetS (RR = 1.27, 95% CI: 1.12–1.44; p &lt; 0.001). With regard to the dietary copper level, 7 studies were included for meta-analysis. The overall multivariable adjusted RR showed that the dietary copper level was inversely associated with MetS (RR = 0.85, 95% CI: 0.78–0.93; p &lt; 0.001). In addition, 4 studies were specified for the dietary selenium level. The overall multivariable adjusted RR indicated that the dietary selenium level was inversely associated with MetS (RR = 0.77, 95% CI: 0.63–0.95; p = 0.01) as well.ConclusionOur results suggest that the dietary iron level is positively associated with MetS, whereas a negative association between the dietary copper and selenium level and MetS is obtained. Further large well-designed prospective cohort studies are warranted to elaborate on the issues examined in this study.
Levels and Health Risk of Pesticide Residues in Chinese Herbal Medicines
Authors: Wang Ying , Gou Yan , Zhang Lei , Li Chun , Wang Zhao , Liu Yuanxi , Geng Zhao , Shen Mingrui , Sun Lei , Wei Feng , Zhou Juan , Gu Lihong , Jin Hongyu , Ma Shuangcheng 
Published: February 2022

In the present study, 168 pesticides in 1,017 samples of 10 Chinese herbal medicines (CHMs) were simultaneously determined by high-performance liquid (HPLC-MS/MS) and gas (GC-MS/MS) chromatography–tandem mass spectrometry. A total of 89.2% of the samples encompassed one or multiple pesticide residues, and the residue concentrations in 60.5% of samples were less than 0.02 mg kg−1, revealing the relatively low residue levels. The hazard quotient and hazard index methods were used to estimate the health risk for consumers. For a more accurate risk assessment, the exposure frequency and exposure duration of CHMs were involved into the exposure assessment, which was obtained from a questionnaire data of 20,917 volunteers. The results of chronic, acute, and cumulative risk assessment indicated that consumption of CHMs is unlikely to pose a health risk to consumers. Ranking the risk of detected pesticides revealed that phorate, BHC, triazophos, methidathion, terbufos, and omethoate posed the highest risk. Our results also showed that pollution of the aboveground medicinal part was more serious. Although exposure to pesticides in tested CHMs was below dangerous levels, more strict controlled management should be carried out for banned pesticides due to the high detection rate and illegal use in the actual planting practice.