bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-Throughput Absolute Quantification Sequencing Reveals that a Combination of Leguminous Shrubs Is Effective in Driving Soil Bacterial Diversity During the Process of Desertification Reversal
Authors: Liu Wangsuo , Qiu Kaiyang , Xie Yingzhong , Huang Yeyun , Wang Ruixia , Li Haichao , Meng Wenfen , He Yi , Li Yayuan , Li Haiquan , Zhao Pengbo , Yang Yi 
Published: December 2022

AbstractDesertification leads to the extreme fragility of ecosystems and seriously threatens ecosystem functioning in desert areas. The planting of xerophytes, especially leguminous shrubs, is an effective and common means to reverse desertification. Soil microorganisms play a crucial role in nutrient cycling and energy flow in ecosystems. However, the effects of introducing leguminous shrubs on soil microbial diversity and the relevant mechanisms are not clear. Here, we employed the high-throughput absolute quantification 16S rRNA sequencing method to analyze the diversity of soil bacteria in sand-fixing areas of mixed shrublands with three combinations of shrubs, i.e., C. korshinskii × Corethrodendron scoparium (CaKCoS), C. korshinskii × Calligonum mongolicum (CaKCaM), and C. scoparium × C. mongolicum (CoSCaM), in the south of the Mu Us Sandy Land, China. This area suffered from moving dunes 20 years ago, but after introducing these shrubs to fix the dunes, the ecosystem was restored. Additionally, the effects of soil physicochemical properties on soil bacterial composition and diversity were analyzed with redundancy analysis (RDA) and structural equation modeling (SEM). It was found that the Shannon index of soil bacteria in CaKCoS was significantly higher than that in CaKCaM and CoSCaM, and the abundance of the dominant phyla, including Actinobacteria, Proteobacteria, Acidobacteria, Chloroflexi, Planctomycetes, Thaumarchaeota, Armatimonadetes, candidate_division_WPS-1, and Nitrospirae, increased significantly in CaKCoS and CaKCaM compared to that in CoSCaM. RDA showed that the majority of soil properties, such as total nitrogen (TN), available potassium (AK), N:P ratio, soil moisture (SM), and available phosphorus (AP), were important soil environmental factors affecting the abundance of the dominant phyla, and RDA1 and RDA2 accounted for 56.66% and 2.35% of the total variation, respectively. SEM showed that the soil bacterial α-diversity was positively affected by the soil organic carbon (SOC), N:P ratio, and total phosphorus (TP). Moreover, CaKCoS had higher SM, total carbon (TC), total potassium (TK), and AP than CaKCaM and CoSCaM. Collectively, these results highlight a conceptual framework in which the combination of leguminous shrubs can effectively drive soil bacterial diversity by improving soil physicochemical properties and maintaining ecosystem functioning during desertification reversal.

Graphical Abstract
Response of Freezing/Thawing Indexes to the Wetting Trend under Warming Climate Conditions over the Qinghai -Tibetan Plateau during 1961–2010: A Numerical Simulation
Authors: Fang Xuewei , Li Zhi , Cheng Chen , Fraedrich Klaus , Wang Anqi , Chen Yihui , Xu Yige , Lyu Shihua 
Published: December 2022

AbstractSince the 1990s, the Qinghai-Tibetan Plateau (QTP) has experienced a strikingly warming and wetter climate that alters the thermal and hydrological properties of frozen ground. A positive correlation between the warming and thermal degradation in permafrost or seasonally frozen ground (SFG) has long been recognized. Still, a predictive relationship between historical wetting under warming climate conditions and frozen ground has not yet been well demonstrated, despite the expectation that it will become even more important because precipitation over the QTP has been projected to increase continuously in the near future. This study investigates the response of the thermal regime to historical wetting in both permafrost and SFG areas and examines their relationships separately using the Community Land Surface Model version 4.5. Results show that wetting before the 1990s across the QTP mainly cooled the permafrost body in the arid and semiarid zones, with significant correlation coefficients of 0.60 and 0.48, respectively. Precipitation increased continually at the rate of 6.16 mm decade−1 in the arid zone after the 1990s but had a contrasting warming effect on permafrost through a significant shortening of the thawing duration within the active layer. However, diminished rainfall in the humid zone after the 1990s also significantly extended the thawing duration of SFG. The relationship between the ground thawing index and precipitation was significantly negatively correlated (−0.75). The dual effects of wetting on the thermal dynamics of the QTP are becoming critical because of the projected increases in future precipitation.
Dietary fat intake is associated with insulin resistance and an adverse vascular profile in patients with T1D: a pooled analysis
Authors: Kietsiriroje Noppadol , Shah Hanya , Zare Marios , O’Mahoney Lauren L. , West Daniel J. , Pearson Sam M. , Ajjan Ramzi A. , Campbell Matthew D. 
Published: December 2022

Abstract
Background
Insulin resistance (IR) increases vascular risk in individuals with Type 1 Diabetes (T1D). We aimed to investigate the relationship between dietary intake and IR, as well as vascular biomarkers in T1D.

Methods
Baseline data from three randomised controlled trials were pooled. Estimated glucose disposal rate (eGDR) was used as an IR marker. Employing multivariate nutrient density substitution models, we examined the association between macronutrient composition and IR/vascular biomarkers (tumour necrosis factor-α, fibrinogen, tissue factor activity, and plasminogen activator inhibitor-1).

Results
Of the 107 patients, 50.5% were male with mean age of 29 ± 6 years. Those with lower eGDR were older with a longer diabetes duration, higher insulin requirements, and an adverse vascular profile (p < 0.05). Patients with higher degrees of IR had higher total energy intake (3192 ± 566 vs. 2772 ± 268 vs. 2626 ± 395 kcal/d for eGDR < 5.1 vs. 5.1–8.6 vs. ≥ 8.7 mg/kg/min, p < 0.001) and consumed a higher absolute and proportional amount of fat (47.6 ± 18.6 vs. 30.4 ± 8.1 vs. 25.8 ± 10.4%, p < 0.001). After adjusting for total energy intake, age, sex, and diabetes duration, increased carbohydrate intake offset by an isoenergetic decrease in fat was associated with higher eGDR (β = 0.103, 95% CI 0.044–0.163). In contrast, increased dietary fat at the expense of dietary protein intake was associated with lower eGDR (β = − 0.119, 95% CI − 0.199 to − 0.040). Replacing fat with 5% isoenergetic amount of carbohydrate resulted in decreased vascular biomarkers (p < 0.05).

Conclusion
Higher fat, but not carbohydrate, intake is associated with increased IR and an adverse vascular profile in patients with T1D.
Fatigue-induced changes in hamstrings’ active muscle stiffness: effect of contraction type and implications for strain injuries
Authors: Evangelidis Pavlos E. , Shan Xiyao , Otsuka Shun , Yang Chi , Yamagishi Takaki , Kawakami Yasuo 
Published: December 2022

Abstract
Purpose
Hamstring strain injuries may occur due to differential fatigue and compromised mechanical properties among the hamstring muscles. We examined (1) the effect of fatigue on hamstrings active muscle stiffness, and (2) whether contraction type affects active muscle stiffness changes during a submaximal fatiguing task.

Methods
Nine healthy males completed 99 submaximal knee flexions in isometric (ISO), concentric (CON), and eccentric (ECC) conditions. We measured the knee flexor maximal voluntary torque (MVT) (pre/post), shear wave velocity (SWV) during contraction and transverse relaxation times (T2) (pre/post) in biceps femoris long head (BFlh), semitendinosus (ST), and semimembranosus (SM) muscles.

Results
MVT decreased substantially after all conditions (− 18.4 to − 33.6%). The average relative torque sustained during the task was lower in CON than ISO and ECC, but absolute torque was similar. SWV interindividual responses were highly variable across muscles and contraction types. On average, BFlh SWV tended to increase in ISO (0.4 m/s, 4.5%, p = 0.064) but decreased in ECC condition (− 0.8 m/s, − 7.7%, p < 0.01). ST SWV decreased in CON (− 1.1 m/s, − 9.0%, p < 0.01), while it remained unchanged in ISO and ECC. SM SWV decreased in CON (− 0.8 m/s, − 8.1%, p < 0.01), but it was unaffected in ISO and variable in ECC.

Conclusion
Fatigue has a differential effect on the mechanical properties of the constituent hamstring muscles, as measured with shear wave elastography, depending upon contraction type. We found preliminary evidence that BFlh is more fatigued than ST or SM during eccentric contractions, which may explain its susceptibility to strain injuries.
Impact of patient nationality on the severity of early side effects after radiotherapy
Authors: Köksal Mümtaz , Streppel Romy , Hauser Stefan , Abramian Alina , Kaiser Christina , Gonzalez-Carmona Maria , Feldmann Georg , Schäfer Niklas , Koob Sebastian , Banat Mohammed , Hamed Motaz , Giordano Frank A. , Schmeel Leonard C. 
Published: December 2022

Abstract
Background
Major demographical changes in Germany commenced in the 1960s. Ongoing humanitarian crises in the Ukraine with subsequent immigration will have also long-range effects on national provision of cancer treatment. Ensuring the best possible outcomes for each cancer patient undergoing radiotherapy requires the prediction and prevention of unfavorable side effects. Given that recent research has primarily focused on clinical outcome indicators solely, less is known regarding sociodemographic predictors of therapeutic outcomes, such as patient nationality. Here, we investigated whether the severity of early side effects after radiotherapy are associated with patient nationality and other sociodemographic and clinical characteristics.

Methods
Out of 9187 patients treated at a German university medical center between 2017 and 2021, 178 German and 178 non-German patients were selected for matched-pair analysis based on diagnostic and demographic criteria. For all 356 patients, data on side effects from follow-up care after radiotherapy were collected.

Results
Non-German patients were more likely to have severe side effects than German patients. Side effect severity was also associated with tumor entity, concomitant therapy, body mass index, and age.

Conclusion
Foreign cancer patients are at higher risk of experiencing severe side effects of radiotherapy, suggesting a need to develop and implement targeted preventive measures for these patients. Further research investigating factors predicting the occurrence of radiotherapy side effects, including other sociodemographic characteristics, is needed to better personalize therapy regimens for cancer.
Gp130 is expressed in pancreatic cancer and can be targeted by the small inhibitor molecule SC144
Authors: Pozios Ioannis , Hering Nina A. , Guenzler Emily , Arndt Marco , Elezkurtaj Sefer , Knösel Thomas , Bruns Christiane J. , Margonis Georgios A. , Beyer Katharina , Seeliger Hendrik 
Published: December 2022

Abstract
Purpose
Interleukin 6 (IL-6), Oncostatin M (OSM), and downstream effector STAT3 are pro-tumorigenic agents in pancreatic ductal adenocarcinoma (PDAC). Glycoprotein 130 (gp130) is a compound of the IL-6 and OSM receptor complex that triggers STAT3 signaling. SC144 is a small molecule gp130 inhibitor with anticancer activity. This study examines the gp130 expression in human PDAC specimens and the in vitro effects of SC144 in PDAC cell lines.

Methods
Tissue micro-arrays were constructed from 175 resected human PDAC. The gp130 expression in tumor epithelium and stroma was determined by immunohistochemistry, and survival analysis was performed. Growth inhibition by SC144 was assessed in vitro using BrdU and MTT assays. Western blotting was performed to evaluate the SC144 effect on IL-6 and OSM signaling.

Results
Gp130 was expressed in the epithelium of 78.8% and the stroma of 9.4% of the tumor samples. The median overall survival for patients with or without epithelial gp130 expression was 16.7 months and 15.9 months, respectively (p = 0.830). Patients with no stromal gp130 expression showed poorer survival than patients with stromal gp130 expression (median 16.2 and 22.9 months, respectively), but this difference did not reach significance (p = 0.144). SC144 inhibited cell proliferation and viability and suppressed IL-6- and OSM-stimulated STAT3Y705 phosphorylation in PDAC cells.

Conclusion
Gp130 is expressed in the epithelium of most human PDAC, but stromal expression is rare. The small molecule gp130 inhibitor SC144 potently inhibits PDAC progression in vitro and may abrogate IL-6 or OSM/gp130/STAT3 signaling. These results suggest gp130 as a novel drug target for pancreatic cancer therapy.
Synthesis and conformational analysis of a potentially super-armed glucuronidation donor
Authors: Steinacher Michael , Svatunek Dennis , Weil Matthias , Mohammadi Bagher , Gärtner Peter 
Published: December 2022

AbstractThe concise synthesis of a potentially “super-armed” glucuronidation donor is reported. The α-anomer was crystallized and analyzed by single crystal X-ray diffraction. The pyranose ring was found to be in a twist-boat conformation in the solid state. To confirm the relevance of this finding for the solution state, and explain the failure of analysis by NMR, DFT calculations were performed. They revealed the twist-boat to be the dominant among a group of several possible conformers at ambient temperature.
Graphical abstract
A systematic review exploring youth peer support for young people with mental health problems
Authors: de Beer C. R. M. , Nooteboom L. A. , van Domburgh L. , de Vreugd M. , Schoones J. W. , Vermeiren R. R. J. M. 
Published: December 2022

AbstractYouth peer support workers (YPSWs) are young adults with lived experience of mental illness during childhood or adolescence who support young people receiving treatment in mental health services. The contributions made by YPSWs are a promising development to facilitate consumer-centered and recovery-oriented care. Although the youth peer support workforce is expanding rapidly, structurally embedding YPSWs in practice is challenging. To overcome these challenges and thereby improve care for young people, insight into YPSW roles, barriers and facilitators for implementing and pursuing youth peer support (YPS) is a necessity. This systematic review examined the published literature to identify existing knowledge on YPSW roles in treatment settings, and the barriers and facilitators for implementing and pursuing YPS in practice. A total of 24 studies from a variety of youth serving contexts were included in this review. Thematic synthesis resulted in six YPSW roles and five themes with barriers and facilitators. The roles included the: engagement role, emotional support role, navigating and planning role, advocacy role, research role and the educational role. The themes explored the needs of YPSWs, experiences of YPSWs, relationships between service users and YPSWs, the collaboration process between YPSWs and non-peer staff, and organizational readiness. This review underlines that YPSWs likely are a valuable addition to numerous youth treatment contexts. Overall, the implementation of YPSWs is a multifaceted operation that requires careful planning. We recommend services to set clear and realistic expectations for YPSWs, to consider potential power imbalances between YPSWs and non-peer staff, to provide adequate resources to pursue YPS, and to approach the implementation of YPSWs with a growth mindset.
Local and Global Canonical Forms for Differential-Algebraic Equations with Symmetries
Authors: Kunkel Peter , Mehrmann Volker 
Published: December 2022

AbstractLinear time-varying differential-algebraic equations with symmetries are studied. The structures that we address are self-adjoint and skew-adjoint systems. Local and global canonical forms under congruence are presented and used to classify the geometric properties of the flow associated with the differential equation as symplectic or generalized orthogonal flow. As applications, the results are applied to the analysis of dissipative Hamiltonian systems arising from circuit simulation and incompressible flow.
The dynamic functions of IRF4 in B cell malignancies
Authors: Maffei Rossana , Fiorcari Stefania , Atene Claudio Giacinto , Martinelli Silvia , Mesini Nicolò , Pilato Flora , Lagreca Ivana , Barozzi Patrizia , Riva Giovanni , Nasillo Vincenzo , Paolini Ambra , Forghieri Fabio , Potenza Leonardo , Trenti Tommaso , Tagliafico Enrico , Luppi Mario , Marasca Roberto 
Published: December 2022

AbstractThe trajectory of B cell development goes through subsequent steps governed by complex genetic programs, strictly regulated by multiple transcription factors. Interferon regulatory factor 4 (IRF4) regulates key points from pre-B cell development and receptor editing to germinal center formation, class-switch recombination and plasma cell differentiation. The pleiotropic ability of IRF4 is mediated by its “kinetic control”, allowing different IRF4 expression levels to activate distinct genetic programs due to modulation of IRF4 DNA-binding affinity. IRF4 is implicated in B cell malignancies, acting both as tumor suppressor and as tumor oncogene in different types of precursors and mature B cell neoplasia. Here, we summarize the complexity of IRF4 functions related to different DNA-binding affinity, multiple IRF4-specific target DNA motif, and interactions with transcriptional partners. Moreover, we describe the unique role of IRF4 in acute leukemias and B cell mature neoplasia, focusing on pathogenetic implications and possible therapeutic strategies in multiple myeloma and chronic lymphocytic leukemia.
Kinematics of the Cervical Spine Under Healthy and Degenerative Conditions: A Systematic Review
Authors: Lindenmann Sara , Tsagkaris Christos , Farshad Mazda , Widmer Jonas 
Published: December 2022

AbstractKnowledge of spinal kinematics is essential for the diagnosis and management of spinal diseases. Distinguishing between physiological and pathological motion patterns can help diagnose these diseases, plan surgical interventions and improve relevant tools and software. During the last decades, numerous studies based on diverse methodologies attempted to elucidate spinal mobility in different planes of motion. The authors aimed to summarize and compare the evidence about cervical spine kinematics under healthy and degenerative conditions. This includes an illustrated description of the spectrum of physiological cervical spine kinematics, followed by a comparable presentation of kinematics of the degenerative cervical spine. Data was obtained through a systematic MEDLINE search including studies on angular/translational segmental motion contribution, range of motion, coupling and center of rotation. As far as the degenerative conditions are concerned, kinematic data regarding disc degeneration and spondylolisthesis were available. Although the majority of the studies identified repeating motion patterns for most motion planes, discrepancies associated with limited sample sizes and different imaging techniques and/or spine configurations, were noted. Among healthy/asymptomatic individuals, flexion extension (FE) and lateral bending (LB) are mainly facilitated by the subaxial cervical spine. C4–C5 and C5–C6 were the major FE contributors in the reported studies, exceeding the motion contribution of sub-adjacent segments. Axial rotation (AR) greatly depends on C1–C2. FE range of motion (ROM) is distributed between the atlantoaxial and subaxial segments, while AR ROM stems mainly from the former and LB ROM from the latter. In coupled motion rotation is quantitatively predominant over translation. Motion migrates caudally from C1–C2 and the center of rotation (COR) translocates anteriorly and superiorly for each successive subaxial segment. In degenerative settings, concurrent or subsequent lesions render the association between diseases and mobility alterations challenging. The affected segments seem to maintain translational and angular motion in early and moderate degeneration. However, the progression of degeneration restrains mobility, which seems to be maintained or compensated by adjacent non-affected segments. While the kinematics of the healthy cervical spine have been addressed by multiple studies, the entire nosological and kinematic spectrum of cervical spine degeneration is partially addressed. Large—scale in vivo studies can complement the existing evidence, cover the gaps and pave the way to technological and clinical breakthroughs.
Rapid extraction of skin physiological parameters from hyperspectral images using machine learning
Authors: Manojlović Teo , Tomanič Tadej , Štajduhar Ivan , Milanič Matija 
Published: December 2022

AbstractNoninvasive assessment of skin structure using hyperspectral images has been intensively studied in recent years. Due to the high computational cost of the classical methods, such as the inverse Monte Carlo (IMC), much research has been done with the aim of using machine learning (ML) methods to reduce the time required for estimating parameters. This study aims to evaluate the accuracy and the estimation speed of the ML methods for this purpose and compare them to the traditionally used inverse adding-doubling (IAD) algorithm. We trained three models – an artificial neural network (ANN), a 1D convolutional neural network (CNN), and a random forests (RF) model – to predict seven skin parameters. The models were trained on simulated data computed using the adding-doubling algorithm. To improve predictive performance, we introduced a stacked dynamic weighting (SDW) model combining the predictions of all three individually trained models. SDW model was trained by using only a handful of real-world spectra on top of the ANN, CNN and RF models that were trained using simulated data. Models were evaluated based on the estimated parameters’ mean absolute error (MAE), considering the surface inclination angle and comparing skin spectra with spectra fitted by the IAD algorithm. On simulated data, the lowest MAE was achieved by the RF model (0.0030), while the SDW model achieved the lowest MAE on in vivo measured spectra (0.0113). The shortest time to estimate parameters for a single spectrum was 93.05 μs. Results suggest that ML algorithms can produce accurate estimates of human skin optical parameters in near real-time.