bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of polycyclic aromatic hydrocarbons (PAHs) bioremediation by hydrocarbonoclastic degrading bacteria (Gordonia terrae)
Authors: Amin A. A. , Wahyuni A. R. T. , Ekawati A.W , Kurniawan A. 
Published: July 2022

Abstract
One of the main focuses in developing bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) is to optimize the hydrocarbonoclastic degrading bacteria such as Gordonia terrae. However, the analysis of the bacteria's capability to degrade PAHs in different concentrations is sparsely explored. This study aims to evaluate the remediation of PAHs by Gordonia terrae by analyzing bacterial activity, PAH degradation, pH, BOD, and COD. The initial PAH concentrations used were 15 ppm, 30 ppm, and 45 ppm for 14 days of incubation. The results show bacterial activity gradually increases in each concentration up to 10-day incubation and decreases in up to 14-day incubation. Moreover, the total PAHs were gradually decreased to 54%, 69%, and 77% in the 15 ppm, 30 ppm, and 45 ppm of initial concentrations, respectively. The final pH values were 6 for all concentrations. At the same time, the BOD and COD values of each concentration gradually decreased until the end of the experiments. This study shows that Gordonia terrae can degrade PAHs, which was achieved optimally after 10 days of incubation. Furthermore, this study indicates that PAH degradation is influenced by bacterial activity, pH, BOD, and COD.
Utilization of mangrove fruit (Rhizophora stylosa) as an alternative to low caffeinated drinks
Authors: Marctyas S A , Pujiastuti D Y , Saputra E 
Published: July 2022

Abstract
A drink that is synonymous with high caffeine content, namely coffee. One way to produce low-caffeine drinks is to make drinks from mangroves (Rhizophora stylosa). The content of compounds in mangrove fruit (Rhizophora stylosa) can be used as processed materials that can be consumed and provide benefits to human health. The purpose of this study was to determine the effect of roasting time on caffeine content. This research was experimental using a Completely Randomized Design (CRD). The treatments used were variations of roasting time, namely P0 (0 minutes), P1 (45 minutes), P2 (50 minutes) and P3 (55 minutes). The results of roasting time has a significant effect on each parameter tested. The treatment at the roasting time of 55 minutes (P3) had the best value on the parameters of colour, flavour, texture, moisture content and caffeine content. In this study, it was found that all treatments of roasting time from P0, P1, P2 and P3 had caffeine content values far below 2%, according to SNI 01-3542-2004 with a maximum of 2% and a minimum of 0,9%. The best content value was at P3 (treatment with roasting time 55 minutes) of 0,814%, which is the best result of low-caffeine mangrove drinks.
Sublethal Toxicity Insecticide Organochlorine Endosulfan on The Value of Blood Sugar Levels and The Level of Life in Tilapia (Oreochromis niloticus) Seeds
Authors: Nurhadi S , Mashitah E D , Sari dan L A 
Published: July 2022

Abstrak
The use of pesticides in agriculture continues to increase. One of the classifications of pesticides is organochlorine endosulfan insecticide that can cause river pollution. One type of fish that is often cultivated in ponds is tilapia. River pollution can trigger stress and even fish death. The stress level of the fish can be seen from the value of its blood sugar levels. This research was conducted on March 20 - April 28, 2021, in Microbiology Laboratory and Anatomy-Aquaculture Laboratory, Faculty of Fisheries and Marine, Universitas Airlangga, to determine the toxicity effect of sublethal endosulfan organochlorine insecticide on blood sugar level value and tilapia seed life rate. This study used experimental methods with CRD through exposure to different organochlorine concentrations, namely P0 (0%), P1 (10%), P2 (30%), P3 (50%), P4 (70%) with four replays. Analysis of the data was processed using ANOVA and DMRT, which showed that sublethal toxicity of endosulfan organochlorine insecticides had an effect (p<0.05) on blood sugar levels and the level of tilapia seed resistance. Using only a concentration of 10% has increased blood sugar levels significantly on the 7th day and continues to increase until the 28th day (114.50 mg/dL). Also, the level of fish life insecurity indicates a value below the Indonesian National Standard (72.50 %).
Chitosan from snapper fish scale waste (Lutjanus spp.) for edible coating
Authors: Florencia A , Putra S E D , Mukti Y P 
Published: July 2022

Abstract
Indonesia is known for its rich fisheries sector. The portion of fish that can be consumed is only 40-50% of the total, the rest is disposed of as waste. Chitosan can be found in fish scales. This study aims to determine the effect of chitosan edible coating from scale waste in extending the shelf life of grapes and the level of consumer acceptance of chitosan edible coating. Chitosan was extracted through deproteination, demineralization, and deacetylation process. Chitosan solutions were made with various concentrations. Based on the results, the chitosan obtained had a yield of 36,32%, water content 3,86%, and ash content 89,54%. In the antibacterial activity test, the inhibition zone formed by chitosan was smaller than 0% chitosan. In the calculation of the De Garmo test obtained the concentration of 0,5% was the best concentration of chitosan. Based on the results of the shelf life estimation test, chitosan coating treatment still cannot replace paraffin wax in extending the shelf life of grapes. In the organoleptic results between samples with coating compared to samples without coating, the results show that the provision of chitosan coating can be accepted by consumers without changing the organoleptic characteristics of the grapes.
Characterization of Tilapia collagen-loaded chitosan nanofibers synthesized by electrospinning method for wound dressing
Authors: Rani C A M , Safira A , Suryadiningrat M , Fikri F , Wardhana D K , Purnama M T E 
Published: July 2022

Abstract
Burns are traumatic injuries that require intensive therapy. Tilapia skin is known to contain high collagen properties and was developed for wound dressing. This study aimed to investigate the characterization of Tilapia collagen-loaded chitosan nanofibers synthesized by electrospinning for wound dressing. This study evaluated three group materials, i.e. (G1) chitosan, (G2) Tilapia collagen, and (G3) Tilapia collagen-loaded chitosan. The electrospun method was performed by alloy the chitosan nanofibers containing 8% w/v Tilapia collagen. The mix solution was characterized using scanning electron microscopy (SEM), Fourier- transform infrared (FTIR) spectroscopy, pH level, swelling test, water capacity test, and cytotoxicity test. As result, Tilapia collagen-loaded chitosan showed the better swelling test, water capacity test, cytotoxicity test, and FTIR evaluation compared to the collagen or chitosan group. However, all group materials showed similarities in pH level and SEM evaluation. In conclusion, Tilapia collagen-loaded chitosan nanofibers could be considered as a wound dressing based on positive results during characterization.
The Effect of Laserpuncture on Accelerate Gonadal Maturity of Female Striped Catfish (Pangasianodon hypophthalmus)
Authors: Patmadevi M , Mukti A T , Mubarak A S , Astuti S D 
Published: July 2022

Abstract
This study aimed to determine the effect of different laserpuncture doses on the gonadal maturity of female striped catfish. The fish 800 to 1000 g with I gonad maturity were used. This study was used a red diode soft-laser with doses of 0.2, 0.4, 0.6, and 0.8 Joule. The positive control was injected chorionic gonadotropin hormone (CGH) and ovaprim™ intramuscular and the negative control was not used any treatment. Red diode soft-laser was shooted at the reproductive point every week for three weeks, while the CGH and ovaprim™ were injected in the final week. Gonad maturity level and gonadosomatic index (GSI) were measured in the last week. The result showed that laserpuncture had significant effect (P<0.05) on the gonadal maturity and GSI of female striped catfish. A dose of 0.4 Joule laserpuncture have the best gonadal maturity level in stage IV. Meanwhile, highest GSI were treated at dose 0.4 Joule laserpuncture, which was 3.4%. Induction of laserpuncture at the reproductive point once a week for three weeks accelerate gonadal maturity of female striped catfish.
The Intensive Program of Hatchery Aquaculture Performance of Gourami (Osphronemus gouramy) in IPB Pandaan
Authors: Pribawastuti V B , Samara S H 
Published: July 2022

Abstract
Gourami is an important economic fish in Indonesia. The increasing demand for gouramy must be balanced with increased seed production by aquaculture activity. One of the important activities in the gouramy aquaculture is hatchery. This study aims to determine the performance of gouramy hatchery at IPB Pandaan. The working method used in this study was descriptive method by utilizing primary and secondary data. The hatchery gouramy in IPB showed good performance with fertilization rate with value of 72.36% and hatching rate of 85.55%. The water quality parameters were also suitable for hatchery gouramy activity. The temperature ranged from 28-29°C, pH levels ranged from 7.1-7.9, DO level ranged from 4.32-5.79 mg/L.
The effect of different maceration solutions towards characteristic gelatin from bone of common carp (Cyprinus carpio)
Authors: Ristyanti E , Triastuti J , Saputra E 
Published: July 2022

AbstractGelatin is a derivative of collagen which is found in connective tissue, skin and bones. Gelatin can be obtained from cow, pig or fish. Teleost fish bones have collagen protein content ranging from 15-17%. Common carp belong to the teleost fish group and research on gelatin from carp bones using different maceration solutions has never been done before. The purpose of this researchment was to determine the effect of different maceration solutions on the characteristics of gelatin from carp bones and to determine their conformity with the standard. This research is a laboratory experimental with Completely Randomized (CRD) consisting of 3 different designs of maceration solutions (NaOH; C6H8O7and a mixture of NaOH and C6H8O7). The results showed that there was an effect in different maceration solutions on the characteristics of carp bone gelatin which was tested with the best treatment using a mixture of NaOH and C6H8O7solutions. Some of the characteristics of gelatin in this researchment are accordance with standards including water content, ash content, viscosity, gel strength, as well as color and pH in several treatments.
The Effect of Rumen’s Cattle Waste as Alternative Nutrient Sources for Thalasiossira sp Aquaculture
Authors: Santanumurti M B , Ogara M I , Santoso L , Hudaidah S 
Published: July 2022

AbstractThe liquid from rumen’s cattle is expected to be used as nutrients in microalgae culture. This study aimed to examine the use of waste of rumen’s cattle as a source of nutrients with different doses in the culture ofThalasiossirasp. The research design used was a completely randomized design (CRD) with 5 treatments with 3 replications of (A) 1 ml conwey/l of seawater, (B) 1 ml of waste/l of sea water, (C) 10 ml of waste/l of seawater, (D) 1 ml of waste + 0.4 gr NaN03/l of seawater, and (E) 10 ml of waste + 0.4 gr NaN03/l of seawater. The calculated parameter were the population density ofThalasiossirasp., diatom diameter, protein content and water quality. The results showed that the waste of rumen’s cattle could be used as an alternative source of nutrients in the culture ofThalasiossirasp. and the peak of the population was reached on day 3. Measurement of protein content ranged from 9.9 to 11.8% and the diameter of the diatoms ranged from 5-10 m. The measurement of the water quality of the media was in the range of temperature 24-26oC, pH value 7 and salinity 32-34 ppt.
Inhibitory Rate of Chitosan Against Vibrio harveyi use in vitro method
Authors: Haryagita  , Kusdarwati R , Suprapto H 
Published: July 2022

Abstract
The intensification of the penaeid shrimp culture industry have been accompanied over the last two decades by an increased incidence of infectious pathogens. Vibrio sp. has become a major source of concern for shrimp culture because of their close association with low survival rates in hatcheries or growout ponds. The antibioctic use to aim this disease but it harmful to use continuously. Resistance of pathogen and retention are such side effect left after antibiotic used. Chitosan is a biocompatible and biodegradable natural polymer with established antimicrobial properties against specific microorganisms. This research demonstrates its antibacterial activity to inhibit growth of Vibrio harveyi comparing with oxoninic acid. Inhibition growth of Vibrio harveyi was tested using diffusion method on TSA added 2% NaCl by additional disc paper which has been soaked in chitosan suspension consentrations 0,8; 1,0; 1,2; 1,4; 1,6% (w/v), asetic acid 1%, and oxolonic acid disc (2μg), each treatment was repeated three times. In this study chitosan showed an antibacterial activity. The lowest antibacterial activity showed by the lowest concentration 0,8% (w/v) and the higest antibacterial activity showed by the highest concentration 1,6% (w/v).
Physical characteristic and antioxidant activities of liquid bath soap with substitution of β-carotene crude extract from Gracilaria sp.
Authors: Ircham M M , Mubarak A S , Saputra E 
Published: July 2022

Abstract
Synthetic antioxidant BHT is widely used in bath soap products as an antioxidant and prevents rancidity in bath soaps, but BHT is harmful to the skin if the addition is excessive. So we need natural antioxidants that can replace the role of BHT as an antioxidant. The purpose of this study was to determine the effect of substitution of BHT with β-carotene Gracilaria sp. on the physical properties of liquid bath soap and determine the best concentration of BHT substitution with β-carotene Gracilaria sp. added to liquid bath soap formulations. This study used a completely randomized design (CRD) consisting of 3 substitution treatments for BHT with β-carotene, namely (1g BHT : 0g β-carotene), (0,5g BHT : 0,5g β-carotene), and (0g BHT : 1g β-carotene) in 100 ml of liquid soap, with 6 repetitions each. Parameters observed were pH, foam stability, viscosity, hedonic, physical stability, and antioxidant activity. The results of this study the substitution of β-carotene Gracilaria sp. the liquid bath soap had a significant effect (p<0.05) on the pH parameter and no significant effect (p<0.05) on the foam stability test and viscosity test parameters. Based on the hedonic test value, soap with the addition of 0,5 g BHT: 0,5g β-carotene has the highest test value to produce the best quality soap.
Cultivation of Phytase-Producing Bacteria as Probiotic Candidate on Molasses and Tempe-Processing Waste
Authors: Gifari Zaid Al , Agistna Ihza , Anwar Khairil , Rosyidi Anwar , Ali Muhamad , Amin Muhamad 
Published: July 2022

Abstract
Microbes that produce phytase enzymes are needed for livestock and fisheries. Animal feed treated with phytase will increase the availability of minerals, amino acids, and energy. For this reason, it is essential to growing phytase-producing bacteria on alternative media that are cheap and easy to obtain. This study aims to determine the ability of some bacteria that produce phytase to grow on alternative media such as molasses and tempeh waste. The indicators used were the growth rate of several phytase-producing bacteria within 3, 4, 5, and 6 hours of culture. The results showed that some bacteria were able to grow on alternative media of tempeh and molasses waste, although slower than growth on commercial media (LB). For this reason, tempeh and molasses waste can be used as alternative media to grow phytase-producing bacteria to produce cheap probiotics for livestock and fisheries.
Reproductive ability of endemic species of Lake Ajamaru Melanotaenia ajamaruensis Allen & Cross, 1980 on spawning at various sex ratio
Authors: Said D S , Mayasari N , Kadarini dan T 
Published: July 2022

Abstract

Melanotaenia ajamaruensis or Ajamaru Rainbowfish is endemic species of Lake Ajamaru, Papua, Indonesia. It belongs to Rainbowfish group, with data deficient status. They have attractive color, especially on male and has potential as an ornamental fish, but the percentage of male is low. A study was conducted to find out their reproductive ability at various sex ratios (male:female = 1:1; 1:2; or 1:3) with two replications for 40 days (January-February 2021) at Research Center for Limnology, LIPI. Observations were also conducted on sexual maturity age, egg diameter, size at a certain age. The ratio of 1:3 have the highest value on number of spawning (NoS): 25 times, number of eggs (NoE)/female: 54 eggs, number of larvae (NoL)/female: 49 larvae, fertilization rate/FR (%): 97.16; hatching rate/HR (%): 97.93; larvae survival at seven days /SR7 (%): 90.34, and eggs incubation time/EIT (days): 5.24. The sexual maturity on 8 months old. Egg diameter (mm): 0.98±0.043, larvae size (mm) at (0 day), and at 1, 2, 4, 8, 12 weeks old were: 3.2±0.68; and 6±0.74; 11.70±1.77; 19.48±1.86; 21.65±2.64; 25.0±2.94 respectively. Daily growth rate 0.26 mm/day That is indicates that M ajamaruensi could adapted well to ex-situ habitat and the reproduction could takes place with a small number of males (1:3).
Length-weight relationships and condition factors of fifteen fish species from Pabean Bay, West Java, Indonesia
Authors: Syafei L S , Sudinno D , Parawangsa I N Y 
Published: July 2022

Abstract
Length-weight relationships (LWR) and condition factors (CF) were calculated for thirteen fish species using 621 specimens from Pabean Bay, West Java, Indonesia. This study is the first reference on length–parameters and condition factors for fifteen species, namely: Ambassis vaschelli, Arius sp., Asterhombus intermedius, Butis amboinensis, Eleuthronema tetradactylum, Gerres abbreviatus, Johnius coitor, Glosogobius sirkumspelitus, Karalla dussumieri, Lutjanus ruselli, Pomadasys kaakan, Pseudorhombus arsius, Sardinella gibbosa, Strongylura strongylura, and Terapon jarbua. Fish caught montly from January to December 2015, using gillnet and set net. The results of LWR and CF from fifteen fish species indicated that the growth pattern was: 3 species were allometric positive (b>3), 1 species was isometric (b=3), and 11 species were allometric negative (b<3).
Study on the potential of sea urchin Tripneustes gratilla (Linnaeus, 1758) as a bioindicator dangerous plastic pollution in environment of gunungkidul beach Yogyakarta
Authors: Pratita S D , Gavintri M B , Rizkyta A N , Khasanah L U , Ponkiyawati F A , Retnoaji B 
Published: July 2022

Abstract
Yogyakarta has a lot of beaches, especially in the Gunungkidul district. Sepanjang and Nglolang are tourist beaches in Gunungkidul, while Porok is a research station for the Faculty of Biology UGM. One common types of waste in the ocean is microplastic. The presence of microplastics in aquatic ecosystems in high numbers can threaten the population of benthic organisms and the stability of the ecosystem. Tripneustes gratilla, one of the sea urchins, can be used as an indicator of environmental pollution because it eats algae and detritus at the bottom of the water. This study aims to determine the microplastic content in seawater, beach substrates, and Tripneustes gratilla organs. Sampling by purposive random sampling, Analysis of microplastics in water, substrate, intestinal and gonads organs. The results obtained that there were four types of microplastics found: fragments, films, fiber, and monofilaments. The most common type of microplastic found is film. The abundance of microplastics on the substrate is higher than that of water. The abundance of microplastics in the intestinal organs of T. gratilla was higher than its gonads. Based on these results, T. gratilla has the potential as a bioindicator of microplastic pollution in the coastal environment of Gunungkidul, Yogyakarta.
Opportunities and its problem for the development of Tilapia aquaculture business in Banjar Regency, South Kalimantan
Authors: Nurlaili N , Hikmah H 
Published: July 2022

Abstract
This paper aims to provide an overview of the potential opportunities for developing tilapia aquaculture and its development problems. The research was conducted in Karang Intan District, Banjar Regency, South Kalimantan in 2021. The method used was a sensus method to 150 respondents and conducting in-depth interviews with representatives of tilapia hatchery business actors in four villages, namely Karang Intan, Padang Panjang, Pandak Daun, and Jingah Habang Ilir. The results of the study indicate that the potential opportunities for developing hatchery aquaculture businesses in Karang Intan District include opportunities for developing aquaculture in Banjar Regency are human resources related to higher education, productive age, mastery of assets and own capital, as well as the availability of adequate land area, potential for a large enough land area owned by business actors, the enthusiasm for work from business actors and the opening of the market. The problems faced include the availability of superior broodstock, the threat of drought, a pandemic disaster due to restrictions, and a flood that only occurred in January this year.
The dominance and proportions of plankton in Pacific white shrimp (Litopenaeus vannamei) ponds cultivated with the intensive system in Bulukumba Regency, South Sulawesi, Indonesia
Authors: Sani M D , Wiradana P A , Maharani A Y , Mawli R E , Mukti A T 
Published: July 2022

Abstract
This study aimed to evaluate plankton in Pacific white shrimp (Litopenaeus vannamei) cultivation in Bulukumba Regency, South Sulawesi. The study was conducted in six intensive ponds for 84 days from November 2019 to February 2020. Plankton samples were collected every 10 days for 8 weeks based on the day of culture (DOC) of Pacific white shrimp since the first rearing in the pond using plankton net (mesh size of 25 μm). Then, planktons were preserved to 5% formalin buffer in 250 mL of sterile plastic. Next, the plankton densities and compositions were analyzed quantitatively and qualitatively. The results showed that plankton dominance in Chlorophyta species and the presence was evenly distributed across all shrimp ponds in the field. The number was relatively stable in all shrimp DOCs and was the highest proportion as well; Chlorophyta (73 to 83%), Diatom group (7.75 to 15.63%), and blue-green algae (BGA) group (7.13 to 13.50%). Plankton can be used as a biomonitor of pollution and shrimp health in dominance and the percentage proportion of each species. Regular monitoring is highly recommended to minimize plankton growth, especially the BGA type that can harm shrimp health in the intensive system.