44 Cocultured Schwann Cells Rescue Irradiated Pelvic Neuron Outgrowth and Increase Survival
Authors: Randolph J , Pak E , McMains J , Koontz B , Hannan J 
Published: April 2022

ABSTRACT

Introduction
Prostatic radiation therapy (RT) leads to erectile dysfunction (ED) by damaging adjacent pro-erectile nerves of pelvic ganglion. Preclinical cell culture studies have shown that RT induces pelvic neuron death and impairs neurite outgrowth. However, ex vivo RT and culture of whole pelvic ganglia leads to enhanced neurite outgrowth. Schwann cells (SC), which are present in intact ganglia, facilitate neuron repair after mechanical injury. The role of SCs in supporting neuron growth and survival in the context of RT has not been investigated.

Objective
Determine if SCs cocultured with primary pelvic neurons improve neuronal survival and growth after ex vivo RT.

Methods
Major pelvic ganglia (MPG) were collected from male Sprague-Dawley rats (n=12) and digested in collagenase/dispase to isolate SCs. SCs were plated on poly-L lysine-coated coverslips and grown in SC-selective medium to confluence for 24 hours. Confluent SCs received RT (0 or 8 Gy) before the addition of neurons. Additional MPGs (n=18) were irradiated (0 or 8 Gy) and digested to isolate pelvic neurons. Dissociated neurons were plated separately or atop SC-coated coverslips to create 6 experimental groups (n=3/grp): 1) CON MPG, 2) RT MPG, 3) CON SC+CON MPG, 4) CONSC+RT MPG, 5) RT SC+CON MPG, and 6) RT SC+RT MPG. After 72 hours, coverslips were fixed and stained for beta-tubulin (neuron marker), S100 protein (myelinating SC marker), neuronal nitric oxide synthase (nNOS; nitrergic marker), tyrosine hydroxylase (TH; sympathetic marker), and TUNEL to assess neurite length, branching, specific neuron populations and apoptosis.

Results
Consistent with previous findings, ex vivo RT decreased MPG neuron length, increased apoptosis and decreased nitrergic neurons in monoculture. In the current study, CON SC+RT MPG cocultures demonstrated increased neurite outgrowth compared to all other groups (p&lt;0.001). Neurite branching was decreased in the RT MPG+RT SC coculture, but unchanged in other cocultures. Groups containing RT MPG neurons exhibited increased proportions of apoptosis, but coculture with CON SC reduced the degree of RT-induced apoptosis (p&lt;0.01). The proportion of adrenergic TH positive neurons was unchanged while nitrergic neurons were significantly lower in RT neurons and coculture with CON SCs was unable to prevent nitrergic loss.

Conclusions
RT induces MPG neuronal apoptosis, inhibits post-injury neuritogenesis, and diminishes erectogenic nitrergic neuron populations. The presence of CON SCs in coculture augments MPG neuron repair mechanisms such as neurite outgrowth and branching. CON SCs partially mitigated overall RT MPG apoptosis, but did not affect the loss of nitrergic neuron populations after RT. These findings suggest that SCs promote MPG neuron survival and facilitate intrinsic repair functions acutely after RT. Further in vivo studies are needed to confirm the potential for healthy SCs to promote pelvic neuron survival and repair following prostatic RT.

Disclosure
No