bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic processes in the body of cows when using organic iodine, microbial synthesis protein and <I>Spirulina platensis</I> in nutrition
Authors: Rykov R. A. 
Published: December 2022

Relevance. Of scientific interest are studies of the integrated use of feed factors aimed at enriching and increasing the biological potential of animal diets.Methods. The scientific and economic experiment was carried out on dairy cows of the Holstein breed at the "Mavrino" farm of the FSUE experimental farm "Klenovo-Chegodaevo" in two groups of 10 animals each. One group was the control group, the other was the experimental group. Cows of the experimental group two weeks before calving and within 100 days after calving received 150 and 300 g of microbial synthesized protein for the main diet, 25 and 50 g of biologically active mixture before and after calving, respectively. The mixture consisted of a granular mass of organic iodine containing 0.5 mg of iodine per 1 g, dry biomass of microalgae Spirulina platensis, dry beer sprouts as a filler in a proportion of 40, 6 and 54%. During the experiment, at the end of the first and third months of lactation following indicators were studied: biochemical indicators; hematological parameters; milk productivity and milk quality, reproduction indicators.Results. The inclusion of microbial synthesizet protein, microalgae Spirulina platensis and organic iodine in the diet of cows during the periparturient period had a positive effect on the interstitial metabolism of proteins, carbohydrates, lipids and minerals, optimized the activity of enzymes and hormones, resulting in the average daily milk yield in cows of the experimental group for three months after calving being 30.6, 33.3 and 27.8 kg, which was higher than that of the controls by 7.7, 7.1 and 14.1%. The cows of the experimental group also improved reproduction rates.
Changes in the concentration of chemical elements in the body tissues of broilers when fed with the probiotic <I>Bacillus cereus</I> and coumarin
Authors: Nurzhanov B. S. , Rakhmatullin Sh. G. , Duskaev G. K. , Levakhin G. I. 
Published: December 2022

Relevance. Due to the cancellation of feeding animals with low doses of antibiotics, alternative substances are being searched, phytobiotics and probiotics may become promising. The use of plant-based feed additives in the nutrition of farm animals and poultry contributes to improving health, and, consequently, improving productive qualities.The purpose of the study: to study the effect of probiotic (Bacillus cereus IP 5832) and coumarin both separately and together on the concentration of chemical elements in the body tissues of broiler chickens.Methods. The object of the study was 7-day-old broiler chickens of the Arbor Acres cross. In our research we used: probiotic based on Bacillus cereus strain and coumarin. Experimental scheme: control group (C) — the main diet (MD), experimental group I — MD + Bacillus cereus (dose of 12.6˟103 microbial bodies/kg of feed/ day), experimental group II — MD + coumarin (dose of 2 mg/kg of feed/day), experimental group III — MD + Bacillus cereus + coumarin. The experiment period is 35 days. Analysis of the elemental composition of tissues was determined by inductively coupled plasma mass spectrometry and inductively coupled plasma atomic emission spectrometry on a "Nexion 300D" quadrupole mass spectrometer and an "Optima 2000 DV" atomic emission spectrometer.Results. As a result of experiments conducted on feeding probiotics to broilers based on Bacillus cereus and coumarin strains, their positive effect on the accumulation of essential and conditionally essential trace elements in the liver and pectoral muscles and a decrease in the presence of a number of toxic elements was established.
Study of the nutritional and productive value of perennial legume-grass mixtures on reclaimed lands of the Upper Volga region
Authors: Kapsamun A. D. , Pavlyuchik E. N. , Ivanova N. N. 
Published: December 2022

Relevance. The article is devoted to the relevant problem of assessing the productive and nutritional value of complex grass mixtures of various families, as well as assessing the economic and agro-energy efficiency of cultivating legume-grass mixtures for fodder.Methods. Experiments were laid at the VNIIMZ experimental agro-reclamation site in 2019–2021. The objects of research were herbal mixtures: 1) oriental goat's rue + cereals (60:40%); 2) variable alfalfa + red clover + meadow timothy (30:35:35%); 3) variable alfalfa + red clover + meadow fescue (30:35:30%); 4) clover-timothy mixture (45:55%). Control — clover-timothy mixture (60:40%), traditional for the production of green mass. The area of the experiment is 6.1 ha, the placement of the options is randomized, in three tiers, the repetition is three times. The use of grass stands is two-cut, agricultural technology is generally accepted. The studies were carried out in accordance with the methodological recommendations adopted in fodder production and agriculture. Statistical processing was carried out by the method of dispersion analysis using computer programs.Results. High yields of grass mixtures based on goat's rue with cereals were obtained — green mass up to 49.9 t/ha, dry matter — up to 14.1 t/ha, while the timothy mixture used as a control — only 23.8 and 6 .78 t/ha, respectively. At the same time, it was found that the collection of fodder protein units, digestible protein and energy productivity were the highest in legume-grass mixed crops based on goat's rue with cereals (13.97 t/ ha, 1.50 t/ha and 167.7 GJ/ha) and grass mixtures based on clover, alfalfa and timothy grass (9.17 t/ha, 1.03 t/ha and 115.2 GJ/ha), while clover-timothy grass mixture provided only 5.49 t/ha, 0.57 t/ha and 58.3 GJ/ ha, respectively (significance of differences p < 0.05). Grass mixtures from goat's rue, alfalfa and red clover with intensive varieties of cereal grasses provide the maximum amount of nutrients per unit area, improve the quality of fodder.
Osmotic stress resistance of different sorghum species affected by NaСl
Authors: Gaze V. L. , Golubova V. A. , Yanovskaya N. V. , Kovtunov V. V. 
Published: December 2022

Introduction. One of the most urgent issue of plant production which attracts the attention of many researchers and farmers, is salt tolerance of plants. Salinity has a negative effect, caused by the disruption of an osmotic balance of a cell that negatively affects water regime of plants. At the same time, plants experience growth inhibition and reduce their yield.The purpose of the study was to estimate resistance of different sorghum species to chloride salinity and to identify the most resistant genotypes.Methods. The study was carried out in the laboratory for plant physiology in 2018–2021. For the current study there has been carries out a modelling of a salt stress by a pure salt solution (concentration of 13 atm.) according to a conventional methodology, the control was distilled water.Results. There has been established the salinity stress effect on seed germination of different sorghum species. The values of germination energy and laboratory germination of grain sorghum were 53.1% and 62.0%; of sweet sorghum — 39.1% and 43.1; the values of Sudan grass — 30.1 and 33.5%, respectively. In the first and second groups — of highly resistant and resistant species (seed germination affected by salinity ranged from 61 to 100%) — were are 61% of grain sorghum genotypes, 56% of sugar sorghum genotypes and 6% of Sudan grass genotypes. There has been established a resistance degree and identified some features of the development of varieties with different degrees of resistance to this stress (length of sprouts and primary roots). Grain sorghum was found less susceptible to salinity stress of all studied species. Among grain sorghum samples, 9 genotypes have demonstrated the highest salinity resistance with a germination rate from 87.5 (Uch. 22/20) to 95.9% (Uch. 29/20). The maximum values were shown by the samples of Uch. 29/20 (95.95%), Uch. 20/20 (93.9%), Uch. 21/20 (93.8%), Jetta x Uch. 45/20 (91.5%) and Uch. 7/20 (90.8%), which indicated the ability to tolerate severe salinity concentration.
Analisis Sentimen Akun Twitter Apex Legends Menggunakan VADER
Authors: Abimanyu Dicky , Budianita Elvia , Cynthia Eka Pandu , Yanto Febi , Yusra Yusra 
Published: December 2022

Abstrak - Pesatnya peningkatan jasa internet saat ini, ada banyak informasi yang dihasilkan dalam jumlah besar secara terus menerus dalam waktu yang singkat. Akhir-akhir ini, analisis sentimen dengan menggunakan ulasan dan pesan telah menjadi topik penelitian yang populer dibicarakan di bidang Natural Language Processing. Selama bertahun-tahun, permainan online telah menjadi suatu aktivitas yang tidak bisa dipisahkan dari sebagian besar orang. Apex Legends adalah salah satu contoh game yang sangat popular di seluruh dunia. Untuk mendapatkan informasi bagaimana pendapat para pemain tentang permainan ini diperlukan analisis sentimen. Pada penelitian ini dilakukan analisis sentimen menggunakan bantuan aplikasi Orange Data Mining dengan metode VADER pada akun twitter Apex Legends menggunakan data sebanyak 500 tweet. Pengujian data dilakukan dengan membandingkan hasil yang didapat menggunakan metode VADER dengan hasil pengujian pakar, yaitu native speaker dari Canada dan Amerika. VADER mengklasifikasikan data yang didapatkan melalui twitter berdasarkan nilai compound yang didapat. Penelitian ini menghasilkan kesimpulan yaitu perbandingan dari pengujian menggunakan VADER dan pengujian pakar tidak berbeda jauh, yang mana total persentase dari penggunaan metode VADER untuk menganalisis sentiment dari twitter ini adalah : Positif = 18%, Negatif = 4,6%, Netral = 73,6%. Sedangkan hasil pengujian pakar adalah : Positif = 27%, Negatif = 10,8%, Netral = 62,2%.Kata kunci: VADER, Apex Legends, Game, Twitter, Uji Pakar Abstract - With the rapid increase in internet services today, there is a lot of information produced in large quantities continuously in a short time. Recently, sentiment analysis using reviews and messages has become a popular research topic discussed in the Natural Language Processing field. Over the years, online gaming has become an activity that cannot be separated from most of the people. Apex Legends is one example of a game that is very popular around the world. To get information on how the players think about the game, sentiment analysis is needed. In this study, sentiment analysis was carried out using the Orange Data Mining application with the VADER method on the Apex Legends twitter account using 500 tweets (data). Data testing is done by comparing the results obtained using the VADER method with the results of expert testing, native speaker from Canada and America. VADER classifies the data obtained through twitter based on the compound value obtained. This study concludes that the comparison of testing using VADER and expert testing is not much different, where the total percentage of using the VADER method to analyze sentiment from Twitter is : Positive = 18%, Negative = 4,6%, Neutral = 73,6%. While the results of expert testing is : Positive = 27%, Negative = 10,8%, Neutral = 62,2%.Keywords : VADER, Apex Legends, Game, Twitter, Expert Test (Uji Pakar)
Beyond Recovery: Colonization, Health and Healing for Indigenous People in Canada
Authors: Lavallee Lynn , Poole Jennifer 
Published: December 2022

<p>How do we limit our focus to mental health when Indigenous teaching demands a much wider lens? How do we respond to mental health recovery when Indigenous experience speaks to a very different approach to healing, and how can we take up the health of Indigenous people in Canada without a discussion of identity and colonization? We cannot, for the mental health and recovery of Indigenous people in Canada have always been tied to history, identity, politics, language and dislocation. Thus, in this paper, our aim is to make clear that history, highlight the impacts of colonization and expound on Indigenous healing practices taking place in Toronto. Based on findings from a local research project, we argue these healing practices go beyond limited notions of recovery and practice, offering profound and practical ways to address the physical, emotional, spiritual and mental health of Indigenous peoples. </p>
Can the nucleic acid Ct value of discharged patients infected with SARS-CoV-2 Omicron variant be 35?——A retrospective study on fluctuation of nucleic acid Ct values in SNIEC mobile cabin hospital
Authors: Zhuang Xu , Zheng Yu , Wei Shun , Zhai Wei , Song Qixiang , Chen Min , Xu Qingrong , Fan Yiling , Zheng Junhua 
Published: December 2022

ObjectiveTo explore the meaning of cycle threshold (Ct) value fluctuation and the appropriateness of setting the discharge Ct value to 35, which is the current standard in Chinese guidelines.MethodA retrospective study was conducted on 95 patients with Ct value fluctuation (Ct value below 35 on day 3; group A) and 97 patients with a normal discharge process (control; group B). Their clinical characteristics and follow-up data were collected.Results(1) There was no significant difference between the groups in age, gender distribution, number of vaccinations, initial ORF-Ct value, and initial N-Ct value. The proportion of patients complicated with chronic internal disorders, respiratory symptoms, and abnormal chest radiology in group A was significantly higher than that in group B. (2) Between the two groups, there was no significant difference in the ORF-Ct or N-Ct value on day 1, but the ORF-Ct and N-Ct values of group B on days 2 to 4 were significantly higher than those of group A. (3) There was no significant difference between the groups in the ORF-Ct value at discharge, but there was a significant difference in the N-Ct value at discharge. Seven days after discharge, almost 100% of the patients had been cured. The mean negative conversion interval of nucleic acid of the patients in group A was 14.5 ± 4.6 days, which was longer than that of the patients in group B (11.8 ± 4 days). (4) Logistic regression analysis showed that the ORF-Ct value on day 2 was the key factor influencing the Ct value fluctuation.ConclusionThe fluctuation of Ct value is only a normal phenomenon in the recovery period of the disease, and there is no need for excessive intervention. It is reasonable to set the Ct value of the discharge standard to 35 and retest the nucleic acid on the 10th day after discharge for patients with underlying diseases or symptoms.
The diversity of vaginal microbiome in women infected with single HPV and multiple genotype HPV infections in China
Authors: Liu Shufa , Li Yuanyue , Song Yuzhu , Wu Xiaomei , Baloch Zulqarnain , Xia Xueshan 
Published: December 2022

IntroductionThe human papillomavirus (HPV) is the leading cause of cervical cancer globally. However, its microbial composition and association with the types of HPV infection remain elusive.MethodsThis study was designed to characterize the vaginal microbiota of 53 HPV-infected and 16 normal women (control group) by using high-throughput sequencing with the Illumina platform.ResultsIn this study, the five leading phyla were Firmicutes (73.9%), Actinobacteriota (12.8%), Proteobacteria (6.2%), Fusobacteria (3.5%), and Bacteroidota (3.1%). We found that single HPV genotype–positive women had higher α-microbial diversity compared with HPV-negative and multiple HPV–positive women. In women with a single HPV genotype infection, the HPV-16 infection had significantly higher α-diversity than other genotype infections. In multiple HPV genotype–positive women, the highest α-diversity was found in women positive for HR–HR HPV genotype infection, compared with other infections. Furthermore, in single- and multiple-genotype infections, the abundance of s_unclassified_g_Lactobacillus decreased whereas the abundance of s_Gardnerella_vaginalis increased compared with control. Additionally, s_unclassified_f_Rhizobiaceae and s_sneathia_sanguinegens were only found in HPV-infected women.ConclusionThis study showed that the type of HPV infection was associated with the composition of the vaginal microbiota. Further studies on HPV genotypes and vaginal microbiota are necessary to uncover more mysteries of their association and provide a promising therapeutic target as well as low-cost future therapeutic strategies.
The attitudes of Jordanian English language and literature undergraduate students toward open-book exams
Authors: Abu-Snoubar Tamador Khalaf , Aldowkat Issam , Al-Shboul Yasser , Atiyat Mona Ahmad , Al-Hyari Hadeel 
Published: December 2022

IntroductionThe paper aims to investigate the attitudes of English Language and Literature students toward implementing the open-book exam in literature courses.MethodsThe research applied a mixed-method approach. The researchers interviewed five undergraduate students enrolled in the American Literature course for the qualitative part. In contrast, the research includes the results of 62 questionnaires for the quantitative part. Moreover, a survey questionnaire was developed by the researchers to collect data concerning the participants’ attitudes toward OBEs.ResultsThe study found a positive attitude toward OBEs. Moreover, the study participants demonstrated that OBEs reduce stress levels that occur during exams in general. A statistical representation of the findings showed that the sample students have a positive attitude in relation to this unconventional assessment protocol.DiscussionOBEs should be allowed more space in academic institutions since today’s world does not depend on the amount of information a job applicant deposits in his memory. What matters is the person possesses enough high-order thinking abilities and skills like problem-solving.
Associations of endogenous androgens and sex hormone-binding globulin with kidney function and chronic kidney disease
Authors: Lau Lina Hui Ying , Nano Jana , Prehn Cornelia , Cecil Alexander , Rathmann Wolfgang , Zeller Tanja , Lechner Andreas , Adamski Jerzy , Peters Annette , Thorand Barbara 
Published: December 2022

IntroductionThe role of endogenous androgens in kidney function and disease has not been extensively explored in men and women.Research design and methodsWe analyzed data from the observational KORA F4 study and its follow-up examination KORA FF4 (median follow-up time 6.5 years) including 1293 men and 650 peri- and postmenopausal women, not using exogenous sex hormones. We examined the associations between endogenous androgens (testosterone [T], dihydrotestosterone [DHT], free T [fT], free DHT [fDHT], and T/DHT), with estimated glomerular filtration rate (eGFR) at baseline and follow-up, prevalent, and incident chronic kidney disease (CKD) adjusting for common CKD risk factors.ResultsAt baseline, 73 men (5.7%) and 54 women (8.4%) had prevalent CKD. Cross-sectionally, no significant associations between androgens and kidney function were observed among men. In women, elevated T (β=-1.305, [95% CI -2.290; -0.320]) and fT (β=-1.423, [95% CI -2.449; -0.397]) were associated with lower eGFR. Prospectively, 81 men (8.8%) and 60 women (15.2%) developed incident CKD. In women, a reverse J-shaped associations was observed between DHT and incident CKD (Pnon-linear=0.029), while higher fDHT was associated with lower incident CKD risk (odds ratio per 1 standard deviation=0.613, [95% CI 0.369; 0.971]. Among men, T/DHT (β=-0.819, [95% CI -1.413; -0.226]) and SHBG (Pnon-linear=0.011) were associated with eGFR at follow-up but not with incident CKD. Some associations appeared to be modified by type 2 diabetes (T2D).ConclusionSuggestive associations are observed of androgens and SHBG with kidney impairment among men and women. However, larger well-phenotyped prospective studies are required to further elucidate the potential of androgens, SHBG, and T2D as modifiable risk factors for kidney function and CKD.
Interplay of four types of RNA modification writers revealed distinct tumor microenvironment and biological characteristics in pancreatic cancer
Authors: Gao Wenzhe , Chen Dongjie , Liu Jixing , Zang Longjun , Xiao Tijun , Zhang Xianlin , Li Zheng , Zhu Hongwei , Yu Xiao 
Published: December 2022

BackgroundPancreatic cancer (PC) is one of the most lethal malignancies and carries a dismal mortality and morbidity. Four types of RNA modification (namely m6A, m1A, APA and A-to-I) could be catalyzed by distinct enzymatic compounds (“writers”), mediating numerous epigenetic events in carcinogenesis and immunomodulation. We aim to investigate the interplay mechanism of these writers in immunogenomic features and molecular biological characteristics in PC.MethodsWe first accessed the specific expression pattern and transcriptional variation of 26 RNA modification writers in The Cancer Genome Atlas (TCGA) dataset. Unsupervised consensus clustering was performed to divide patients into two RNA modification clusters. Then, based on the differentially expressed genes (DEGs) among two clusters, RNA modification score (WM_Score) model was established to determine RNA modification-based subtypes and was validated in International Cancer Genome Consortium (ICGC) dataset. What’s more, we manifested the unique status of WM_Score in transcriptional and post-transcriptional regulation, molecular biological characteristics, targeted therapies and immunogenomic patterns.ResultsWe documented the tight-knit correlations between transcriptional expression and variation of RNA modification writers. We classified patients into two distinct RNA modification patterns (WM_Score_high and _low), The WM_Score_high subgroup was correlated with worse prognosis, Th2/Th17 cell polarization and oncogenic pathways (e.g. EMT, TGF-β, and mTORC1 signaling pathways), whereas the WM_Score_low subgroup associated with favorable survival rate and Th1 cell trend. WM_Score model also proved robust predictive power in interpreting transcriptional and post-transcriptional events. Additionally, the potential targeted compounds with related pathways for the WM_Score model were further identified.ConclusionsOur research unfolds a novel horizon on the interplay network of four RNA modifications in PC. This WM_Score model demonstrated powerful predictive capacity in epigenetic, immunological and biological landscape, providing a theoretical basis for future clinical judgments of PC.