bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biofilm and hyphal inhibitory synergistic effects of phytoactives piperine and cinnamaldehyde against Candida albicans
Authors: Priya Arumugam , Pandian Shunmugiah Karutha 
Published: June 2022

Abstract

Oral candidiasis, the most common mycotic infection of the human oral cavity is non-life-threatening yet, if untreated, may advance as systemic infections. The ability of Candida albicans to adapt sessile lifestyle imparts resistance to drugs and host immunity. Consequently, due to the limited effectiveness of conventional antifungal treatment, novel therapeutic strategies are required. In the present study, synergistic interaction of phytochemicals, piperine, and cinnamaldehyde against the biofilm and hyphal of C. albicans was evaluated. Minimum inhibitory concentration (MIC) and biofilm inhibitory concentration (BIC) of piperine and cinnamaldehyde against C. albicans were analyzed through microbroth dilution assay and crystal violet staining method, respectively. Combinatorial biofilm and hyphal inhibitory effect were investigated through checkerboard assay. In vitro results were validated through gene expression analysis. BIC of piperine and cinnamaldehyde was determined to be 32 μg/ml and 64 μg/ml, respectively. Interaction between these two phytocomponents was found to be synergistic and six different synergistic antibiofilm combinations were identified. Microscopic analysis of biofilm architecture also evidenced the biofilm and surface adherence inhibitory potential of piperine and cinnamaldehyde combinations. Phenotypic switching between yeast and hyphal morphological forms was influenced by synergistic combinations. qPCR analysis corroborated the results of in vitro activities. nrg1 and trp1, the negative transcriptional regulators of filamentous growth were upregulated whereas other genes that are involved in biofilm formation, filamentous growth, adhesion, etc. were found to be downregulated. These proficient phytochemical combinations provide a new therapeutic avenue for the treatment of biofilm-associated oral candidiasis and to combat the recurrent infections due to antibiotic resistance.

Lay abstract
VUV photopolymerization of ketene under interstellar conditions: from the dilute phase to the condensed phase
Authors: Ibrahim Mohamad , Guillemin Jean-Claude , Krim Lahouari 
Published: June 2022

ABSTRACT
The photodecomposition of ketene under interstellar conditions and how the resulting photofragments may recombine in the 3–300 K temperature range could play an important role in investigations related to astrochemistry and astrobiology. Using a combination of bulk ice and rare-gas matrix isolation studies coupled to Fourier transform infrared (FTIR) spectroscopy, this work aims to understand the vacuum ultraviolet (VUV) photochemistry of CH2CO in solid phase to mimic the photochemistry of organic species trapped in the icy interstellar grains. We show that the photolysis of CH2CO depends strongly on the environments where it is trapped. The VUV photolysis of CH2CO/Ne in dilute phase leads to kinetically stable and unstable species such as CO, C2H2, CH4, C2H4, C2H6, H2CO, CH3CHO, HCCO, C2O, C3O, and C4O. However, the same experiment carried out in condensed phase shows that the photolysis of CH2CO ice produces mainly an organic residue, which is directly observed at 10 K and remains stable in solid phase at 300 K. The infrared (IR) spectroscopy analysis suggests that the resulting organic residue could be a polyketone formed at 10 K through the VUV photopolymerization of ketene.
A case of aortoduodenal fistula caused by IgG4-related periaortitis
Authors: Oka Hideki , Sumitomo Shuji , Shimizu Hayato , Kanamori Maki , Yamashita Daisuke , Nishioka Hiroaki , Ohmura Koichiro 
Published: June 2022

ABSTRACT
An 86-year-old man who underwent endovascular aortic repair for impending rupture of an abdominal aortic aneurysm a year ago presented to our hospital because of fatigue and black stools. Multiple bacterial specimens were detected in blood cultures, and computed tomography following oral administration of gastrografin demonstrated gastrografin in the abdominal aorta. The diagnosis of aortic duodenal fistula was confirmed and emergency abdominal aortic replacement was performed. The pathological findings of the aorta included a large number of immunoglobulin G4 (IgG4)-positive plasma cells infiltrating all layers of the aortic wall, with particularly marked thickening of the adventitia. The serum IgG4 level was 241 mg/dl and IgG4-related periaortitis was diagnosed. Aortoduodenal fistula is a rare but fatal complication of IgG4-related periaortitis. Patients should be followed carefully after endovascular aortic repair for inflammatory abdominal aortic aneurysms.
Epigenetic dysregulation in meningiomas
Authors: Wedemeyer Michelle A , Muskens Ivo , Strickland Ben A , Aurelio Oscar , Martirosian Vahan , Wiemels Joseph L , Weisenberger Daniel J , Wang Kai , Mukerjee Debraj , Rhie Suhn K , Zada Gabriel 
Published: June 2022

Abstract

Background
Meningiomas are the most common primary brain tumor. Though typically benign with a low mutational burden, tumors with benign histology may behave aggressively and there are no proven chemotherapies. Although DNA methylation patterns distinguish subgroups of meningiomas and have higher predictive value for tumor behavior than histologic classification, little is known about differences in DNA methylation between meningiomas and surrounding normal dura tissue.

Methods
Whole-exome sequencing and methylation array profiling were performed on 12 dura/meningioma pairs (11 WHO grade I and 1 WHO grade II). Single-nucleotide polymorphism (SNP) genotyping and methylation array profiling were performed on an additional 19 meningiomas (9 WHO grade I, 5 WHO grade II, 4 WHO grade III).

Results
Using multimodal studies of meningioma/dura pairs, we identified 4 distinct DNA methylation patterns. Diffuse DNA hypomethylation of malignant meningiomas readily facilitated their identification from lower-grade tumors by unsupervised clustering. All clusters and 12/12 meningioma-dura pairs exhibited hypomethylation of the gene promoters of a module associated with the craniofacial patterning transcription factor FOXC1 and its upstream lncRNA FOXCUT. Furthermore, we identified an epigenetic continuum of increasing hypermethylation of polycomb repressive complex target promoters with increasing histopathologic grade.

Conclusion
These findings support future investigations of the role of epigenetic dysregulation of FOXC1 and cranial patterning genes in meningioma formation as well as studies of the utility of polycomb inhibitors for the treatment of malignant meningiomas.
Leptospirosis in Retirees Living in Rural Areas: A Poorly Recognized Emerging Problem in Mainland France?
Authors: Vibert Mathilde , Guimard Thomas , Brochard Julia , Takoudju Eve-Marie , Larrose Catherine , Boutoille David , Le Turnier Paul 
Published: June 2022

Abstract
Among 40 patients diagnosed with leptospirosis in 3 hospitals of western mainland France between 2014 and 2018, half were at least 60 years old and retired. Their exposure factors were mainly rural residential environment with limited remarkable risk factors. Better awareness and information on leptospirosis appear necessary in this population.
Leaf cell-specific and single-cell transcriptional profiling reveals a role for the palisade layer in UV light protection
Authors: Procko Carl , Lee Travis , Borsuk Aleca , Bargmann Bastiaan O R , Dabi Tsegaye , Nery Joseph R , Estelle Mark , Baird Lisa , O’Connor Carolyn , Brodersen Craig , Ecker Joseph R , Chory Joanne 
Published: June 2022

Abstract
Like other complex multicellular organisms, plants are composed of different cell types with specialized shapes and functions. For example, most laminar leaves consist of multiple photosynthetic cell types. These cell types include the palisade mesophyll, which typically forms one or more cell layers on the adaxial side of the leaf. Despite their importance for photosynthesis, we know little about how palisade cells differ at the molecular level from other photosynthetic cell types. To this end, we have used a combination of cell-specific profiling using fluorescence-activated cell sorting and single-cell RNA-sequencing methods to generate a transcriptional blueprint of the palisade mesophyll in Arabidopsis thaliana leaves. We find that despite their unique morphology, palisade cells are otherwise transcriptionally similar to other photosynthetic cell types. Nevertheless, we show that some genes in the phenylpropanoid biosynthesis pathway have both palisade-enriched expression and are light-regulated. Phenylpropanoid gene activity in the palisade was required for production of the ultraviolet (UV)-B protectant sinapoylmalate, which may protect the palisade and/or other leaf cells against damaging UV light. These findings improve our understanding of how different photosynthetic cell types in the leaf can function uniquely to optimize leaf performance, despite their transcriptional similarities.
Controllable synthesis of layered black bismuth oxidechloride nanosheets and their applications in internal tumor ablation
Authors: Fang Qianlan , Xu Yu , Luo Lijia , Liu Chuang , Li Zihou , Lin Jie , Chen Tianxiang , Wu Aiguo 
Published: June 2022

Abstract
The recently emerging bismuth oxyhalide (BiOX) nanomaterials are promising indirect band gap photosensitizer for ultraviolet (UV) light-triggered phototherapy due to their unique layered nanosheet structure. However, the low absorption and poor photothermal conversion efficiency have always impeded their further applications in cancer clinical therapy. Herein, BiOCl rich in oxygen vacancies has been reported to have full-spectrum absorption properties, making it possible to achieve photothermal property under near-infrared laser. Under 808 nm irradiation, the photothermal conversion efficiency of black BiOCl nanosheets (BBNs) is up to 40%. BBNs@PEG can effectively clear primary subcutaneous tumors and prevent recurrence, achieving good synergistic treatment effect. These results not only broke the limitation of UV on the BiOCl material and provided a good template for other semiconductor materials, but also represent a promising approach to fabricate BBN@PEG a novel, potent and multifunctional theranostic platform for precise photothermal therapy and prognostic evaluation.
Three-dimensional bioprinting sodium alginate/gelatin scaffold combined with neural stem cells and oligodendrocytes markedly promoting nerve regeneration after spinal cord injury
Authors: Liu Shuo , Yang Hui , Chen Dong , Xie Yuanyuan , Tai ChenXu , Wang Liudi , Wang Peng , Wang Bin 
Published: June 2022

Abstract
Accumulating research has indicated that the transplantation of combined stem cells and scaffolds is an effective method for spinal cord injury (SCI). The development of three-dimensional (3D) bioprinting technology can make the 3D scaffolds combined with cells more accurate and effective for SCI treatment. However, unmyelinated newborn nerve fibers have no nerve signaling conduction, hampering recovery of motor function. In this study, we designed and printed a type of sodium alginate/gelatin scaffold loaded with neural stem cells and oligodendrocytes, which were involved in the formation of the myelin sheaths of neural cell axons. In order to observe the effectiveness of this 3D bioprinting scaffold, we transplanted it into the completely transected rat spinal cord, and then immunofluorescence staining, hematoxylin–eosin staining and behavioral assessment were performed. The results showed that this 3D bioprinting scaffold markedly improved the hindlimb motor function and promoted nerve regeneration. These findings suggested that this novel 3D bioprinting scaffold was a good carrier for cells transplantation, thereby enhancing spinal cord repair following injury.
The Value of Differing Points of View: Evidence from Financial Analysts’ Geographic Diversity
Authors: Gerken William C , Painter Marcus O 
Published: June 2022

Abstract
Using satellite imagery of retail firms’ parking lots to measure time-varying local firm-specific performance, we document that analysts incorporate local information into their forecasts. Analysts rely more on local signals when less firm-wide information is available. This incorporation of noisy local firm information has firm-level implications. Examining across industries, we find causal evidence that geographic concentration of analysts increases consensus forecast errors and decreases firm liquidity. These effects are stronger for harder-to-value stocks. The market values geographic firm information, as the abnormal return around forecast revisions is higher for analysts who cover a firm from a unique location.
Gestational Age-Specific Nomogram of Transcutaneous Bilirubin in First 120 h of Life for Term and Late Preterm Indian Neonates
Authors: Shaw Subhash Chandra , Negi Vandana , Bhat Vivek , Kumar Ashutosh , Joshi Rajneesh K , Venkatnarayan Kannan , Gupta Rakesh , Kunwar Bishwo Raj Bdr , Gopalakrishnan Shridhar , Tewari Vishal Vishnu , Kanitkar Madhuri 
Published: June 2022

Abstract

Objective
To create a nomogram based on transcutaneous bilirubin values (TCB) in first week of life for term and late preterm (&gt;34 weeks) neonates.

Methods and design
Prospective longitudinal study.

Setting
Four tertiary-care teaching hospitals (one each in eastern and southern India, two in northern India) between February 2019 and March 2020.

Participants
A total of 2492 term and late preterm (&gt;34 weeks) neonates.

Intervention
Bilirubin was measured by transcutaneous bilirubinometer (Drager JM-105, Germany) in all neonates in pre-specified times of the day, 12 hourly every day since birth till discharge between 48 and 72 h, and data were recorded in epochs of 6 hourly intervals. Post-discharge, all neonates were called for review in next 48 h.

Outcome measures
Primary—TCB in first week of life. Secondary—factors having significant association with significant hyperbilirubinaemia requiring phototherapy.

Results
Total of 2492 neonates (males 1303 and female 1189), with a total of 14 162 TCB recordings were analysed and mean hourly bilirubin (TCB) at hourly intervals till 120 h and then daily bilirubin values on Days 6 and 7 were tabulated. We have constructed hour-specific bilirubin nomogram with percentiles as per gestational age in term and near-term Indian neonates till 120 h of life. Amongst the known risk factors, delayed cord clamping, primipara and breastfeeding jaundice had significant association for hyperbilirubinaemia needing phototherapy.

Conclusions
We have created gestation-specific nomogram of TCB levels in 6 hourly intervals for the first 120 postnatal hours, obtained from a large predominantly breast fed healthy, term and near-term Indian neonates.
Emerging approaches in the diagnosis and therapy in shellfish allergy
Authors: Wai Christine Y.Y. , Leung Patrick S.C. 
Published: June 2022

Purpose of review
Despite the high prevalence of shellfish allergy, the clinical management of seafood allergy has remained unchanged over decades. Here, we examined the current status in the diagnosis and clinical management of shellfish allergy and highlighted the imminent need for more specific diagnostic methods, as well as effective and safe therapeutic approaches for shellfish allergy.

Recent findings
With the advancement in the molecular identifications and definition of reactive epitopes of shellfish allergens, new diagnostic designs such as component-resolved diagnosis, basophil activation test (BAT) and the emerging IgE-crosslinking-induced luciferase expression are emerging. Furthermore, various allergen-specific immunotherapy strategies (such as shellfish extracts and allergens, hypoallergens, hypoallergen DNA vaccines, mimotopes and peptide-based therapies) are being explored at preclinical stages whereas limited nonallergen specific immunotherapy approaches are under clinical trials.

Summary
With an increasing understanding of the underlying immunological mechanisms and molecular features of shellfish allergy, the future for developing precise diagnostic and therapeutic strategies to better manage shellfish allergy is promising.
Identifying Mental Health Needs in the Faith Community
Authors: Gallo Veronica 
Published: June 2022

ABSTRACT:
Nearly one in five Americans suffers from a mental health disorder, and mental health issues have escalated during the COVID-19 pandemic. As trusted members of the community, faith community nurses are in a key position to assess mental health needs and take leadership roles in the faith community to address these needs. The aim of this project was to assess the mental health needs of one faith community. Qualitative data with quantitative data from secondary sources were gathered. Analysis revealed three problems for those suffering from mental health disorders: lack of resilience and coping strategies, fear and/or ignorance of mental health disorders, and unfamiliarity with community and health resources.
Cultivating Students' Practice of Prayer with Patients
Authors: Keesee Lea , Nease Sandy , Gallegos Belinda 
Published: June 2022

ABSTRACT:
Educating prelicensure nurses about the positive outcomes of prayer can improve the potential for nurses to develop their skill and readiness to pray with or for patients as is appropriate and ethical. When nursing students learn to pray with their patients and families, the trusting component of the nurse-patient relationship becomes strengthened. As faculty and preceptors role-model praying with patients, students can begin to develop competency in providing spiritual care.
Internet of Things for beyond-the-laboratory prosthetics research
Authors: Wu Hancong , Dyson Matthew , Nazarpour Kianoush 
Published: June 2022

Research on upper-limb prostheses is typically laboratory-based. Evidence indicates that research has not yet led to prostheses that meet user needs. Inefficient communication loops between users, clinicians and manufacturers limit the amount of quantitative and qualitative data that researchers can use in refining their innovations. This paper offers a first demonstration of an alternative paradigm by which remote, beyond-the-laboratory prosthesis research according to user needs is feasible. Specifically, the proposed Internet of Things setting allows remote data collection, real-time visualization and prosthesis reprogramming through Wi-Fi and a commercial cloud portal. Via a dashboard, the user can adjust the configuration of the device and append contextual information to the prosthetic data. We evaluated this demonstrator in real-time experiments with three able-bodied participants. Results promise the potential of contextual data collection and system update through the internet, which may provide real-life data for algorithm training and reduce the complexity of send-home trials.
This article is part of the theme issue ‘Advanced neurotechnologies: translating innovation for health and well-being’.
Cleanroom strategies for micro- and nano-fabricating flexible implantable neural electronics
Authors: Walton Finlay , Cerezo-Sanchez Maria , McGlynn Eve , Das Rupam , Heidari Hadi 
Published: June 2022

Implantable electronic neural interfaces typically take the form of probes and are made with rigid materials such as silicon and metals. These have advantages such as compatibility with integrated microchips, simple implantation and high-density nanofabrication but tend to be lacking in terms of biointegration, biocompatibility and durability due to their mechanical rigidity. This leads to damage to the device or, more importantly, the brain tissue surrounding the implant. Flexible polymer-based probes offer superior biocompatibility in terms of surface biochemistry and better matched mechanical properties. Research which aims to bring the fabrication of electronics on flexible polymer substrates to the nano-regime is remarkably sparse, despite the push for flexible consumer electronics in the last decade or so. Cleanroom-based nanofabrication techniques such as photolithography have been used as pattern transfer methods by the semiconductor industry to produce single nanometre scale devices and are now also used for making flexible circuit boards. There is still much scope for miniaturizing flexible electronics further using photolithography, bringing the possibility of nanoscale, non-invasive, high-density flexible neural interfacing. This work explores the fabrication challenges of using photolithography and complementary techniques in a cleanroom for producing flexible electronic neural probes with nanometre-scale features.
This article is part of the theme issue 'Advanced neurotechnologies: translating innovation for health and well-being'.
An affordable implantable vagus nerve stimulator system for use in animal research
Authors: Canal-Alonso Ángel , Casado-Vara Roberto , Castellano Orlando , Herrera-Santos Jorge , Gonçalves Jaime , Màrquez-Sànchez Sergio , Gonçalves Jesús María , Corchado Juan Manuel 
Published: June 2022

In this research, a vagus nerve stimulator has been developed and miniaturized for use in epilepsy research. The board contains all the components necessary for its operation during the standard duration of the experiments, being possible to control it once implanted and even being able to reuse it. The VNS system has been designed for rodents since the VNS devices available for human are not only too large for laboratory animals, but also too expensive. With this solution the expenditure on materials made by laboratories is greatly reduced and bioethical considerations were kept in mind. The system was validated in hamsters.
This article is part of the theme issue ‘Advanced neurotechnologies: translating innovation for health and well-being’.
Canadian Healthcare Providers’ Attitudes Towards Automated Insulin Delivery Systems
Authors: Morrison Amy E , Farnsworth Kate , Witteman Holly O , Lam Anna , Senior Peter A 
Published: June 2022

AbstractIntroductionWe aimed to assess the current experience and attitudes towards Commercial and Do-it-yourself (DIY) automated insulin delivery (AID) systems among healthcare providers (HCP) across Canada.MethodsA cross-sectional study was performed through electronic distribution of an anonymous survey to HCP licensed to practice in Canada looking after people with type 1 diabetes (T1D).ResultsResponses included 204 HCP across the multi-disciplinary team; dieticians (32.8%), nurses (31.9%), and endocrinologists (28.4%), looking after adults (51%) and children (23%) mainly in urban areas (85.7%). Respondents reported a median 100-500 patients with T1D per practice, with a median 6-24 current users/practice of Commercial compared to a median 1-5 current users/practice of DIY AID. The majority of HCP (72.7%) were comfortable supporting Commercial AID, whereas only 21.6% reported comfort supporting DIY AID use. A significant, although moderate correlation between HCP experience and comfort was seen; Commercial r=0.57(p<0.0001) and DIY r=0.45(p<0.0001). Respondents reported more barriers to DIY, relative to Commercial AID(p=0.001); unfamiliarity/lack of exposure and medico-legal risks were highlighted with DIY systems. Respondents suggested AID system education (both Commercial and DIY), for HCP and users, to improve HCP confidence.ConclusionsDespite documented beneficial outcomes, AID systems are not widely used in the management of T1D in Canada. The need for both user and HCP education to improve familiarity with the systems, in addition to clarity in medico-legal guidance, have been identified as gaps, which if addressed, might enable the benefits of AID to be more widely available to people with T1D in Canada.