bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pan-cancer analyses reveal molecular and clinical characteristics of cuproptosis regulators in cancer
Authors: Wu Changwu , Tan Jun , Wang Xiangyu , Qin Chaoying , Long Wenyong , Pan Yimin , Li Yuzhe , Liu Qing 
Published: May 2022

Abstract
Imbalance in copper homeostasis can be lethal to the body. A recent study found that excess copper induces cell death in a way that has never been characterized before, which is dependent on mitochondrial stress and referred to as “cuproptosis.” The role of cuproptosis in tumors has not yet been elucidated. In this study, we revealed the complex and important roles of cuproptosis regulators and cuproptosis activity in tumors via a comprehensive analysis of multi-omics data from more than 10 000 samples of 33 tumor types. We found that the cyclin-dependent kinase inhibitor 2A is the most frequently altered cuproptosis regulator, and the cuproptosis regulator expression is dysregulated in various tumors. Additionally, we developed a cuproptosis activity score to reflect the overall cuproptosis level. Based on the expression levels of cuproptosis regulators, tumors can be divided into two clusters with different cuproptosis activities and survival outcomes. Importantly, cuproptosis activity was found to be associated with the prognosis of multiple tumors and multiple tumor-related pathways, including fatty acid metabolism and remodeling of the tumor microenvironment. Furthermore, cuproptosis extensively increased the sensitivity to multiple drugs and exhibited potential to predict the outcome of immunotherapy. We also comprehensively identified cuproptosis-related microRNAs, long non-coding RNAs, and transcription factors. In summary, this study reveals important molecular and clinical characteristics of cuproptosis regulators and cuproptosis activity in tumors, and suggests the use of cuproptosis as a promising tumor therapeutic approach. This study provides an important reference point for future cuproptosis-related research.
‹Ins Off zu Lehren ist wie liebevoll-aufwändiges Kochen für Freunde, ohne das Verspeisen zu erleben›
Authors: Carolus Astrid , Münch Catharina 
Published: May 2022

Die COVID-19-Krise offenbarte Mängel in der Digitalisierung der Schulen, die seither intensiv diskutiert wurden. Diese Studie argumentiert für eine Perspektiverweiterung, die über die Ebene der technischen Ausstattung hinausgeht: In der Analyse des Verhaltens, der Kognitionen und Emotionen von Lehrkräften wird ein forschungsheuristisch fruchtbarer Ansatz für die Untersuchung von Faktoren erkannt, die der mangelnden Digitalisierung potenziell zugrunde liegen. In einer Kombination aus einer qualitativen und quantitativen Onlineerhebung fragt die Studie N = 50 Lehrkräfte nach ihrem Umgang mit dem Fernunterricht während der COVID-19-Pandemie sowie nach ihrer Perspektive auf digitale Medien und Technik. Die Ergebnisse geben einen Einblick in die medienbezogene Unterrichtsgestaltung in der COVID-19-Krise, die Bewertung des Fernunterrichts, weiter in die Nutzung und Bewertung von Medien. Zudem wird gezeigt, dass zwei psychologische Faktoren – «Persönliche Entfaltung» und «Änderungsbereitschaft» – signifikant mit der Technikbereitschaft der Lehrkräfte zusammenhängen. Die Studie leistet insgesamt einen ersten Beitrag zur Analyse der Situation der Lehrkräfte in der gegenwärtigen Pandemie und präsentiert Implikationen sowohl für zukünftige Forschung als auch für die Praxis der Fortbildung der Lehrkräfte.
Facing global transitions in water management: Advances in knowledge and capacity development and towards adaptive approaches
Authors: Alaerts G. J. , Kaspersma J. M. 
Published: May 2022

AbstractThe significance, approaches, and instruments of knowledge and capacity development (KCD) in water management are reviewed, and priorities for the future are proposed. These concepts have become more mainstream, critical in helping water organisations decide on and implement policies, and generating economic returns. Their application still tends to be often limited to education or ‘training’. KCD requires an understanding of the physical world, how institutions must be strengthened to manage it, and how pedagogical and knowledge-management tools, in turn, strengthen the institutions. The private sector first applied knowledge management. The international development theory highlighted the deficiency of governments in implementation capacity. The health and environmental communities are developing an ‘implementation science’ to enhance the capacity to operationalise know-how faster. Advances in KCD include the following: (1) knowledge and capacity converging in nested levels (individual, organisational, institutional, and societal) to cause effective action; (2) six arenas/contexts of KCD application; and (3) pedagogy and knowledge-management through which learning occurs and knowledge is imparted. KCD is a ‘sticky’, slow process. Policy analyses tend to overlook the role of KCD. The water sector is facing acute challenges: a new one of building resilient water-and-land systems and adapting to climate change, and the outstanding one of achieving Sustainable Development Goals (SDGs). Thus, the current KCD must be scaled up, and also structured with a longer-term perspective to support change and reform at policy and organisational levels building on iterative adaption. Policies should become pro-active shaped by modelled forecasts; and organisations more able to change and adapt to future scenarios that are complex, uncertain, and evolving rapidly. Enhancing the capacity to implement policies, establish ‘learning organisations’, and design iterative adaptive pathways requires sustained political commitment. While adopting a long-term programme, supportive KCD activities should stay realistic and manageable.
Prediction of soil water characteristic curves based on low suction section and inflection point
Authors: Zhao Wenju , Ma Feng , Hu Jiazhen , Shi Fuyuan , Wang Yali 
Published: May 2022

Abstract
Given the cumbersome determination method of the Soil Water Characteristic Curve (SWCC), the collapsible loess (silty clay loam) in Lanzhou was taken as the research object to explore a symmetrical prediction method for SWCC in a low suction section on the inflection point, and to determine the optimal suction section and the inflection point. The results showed that in the range of 0–7,000 cm suction, the spatial variation coefficient (CV) of soil saturation of each bulk density increased with the increase of suction. Soil saturation showed weak spatial variability when suction &lt;800 cm, and moderate spatial variability when suction ≥800 cm. Using a bulk density of 1.58 g/cm3 as an example, the SWCC determined by the symmetry of the bending point was compared with the measured data of 0–300, 0–500, 0–800 and 0–1,000 cm suction sections. It was found that the measured soil saturation of SWCC determined by the data for the 0–800 cm suction section was the most consistent with the predicted value. The measured and predicted saturation points of the SWCC were most consistent with suction segments of 0–800 cm. SWCC data of different textures and bulk density were used to verify the prediction method at low suction section and an inflection point of 0–800 cm. It was found that the average absolute error and root mean square error of statistical indicators were close to 0, and the correlation coefficient was greater than 0.9915. The actual and predicted values of each soil parameter were linearly correlated. This method of predicting SWCCs with low suction and inflection points ensures both a high degree of curve fitting and the accuracy of characteristic soil parameters, providing a simple method for the prediction of SWCCs and guidance for managing soil water in loessial areas.
Water criteria evaluation for drinking and irrigation purposes: a case study in one of the largest rivers of Sundarbans World Heritage region
Authors: Hasan Md. Mahabub , Shuvho Md. Bengir Ahmed , Chowdhury Mohammad Asaduzzaman , Alam AKM Mashud , Hassan Mahamudul , Hossain Nayem 
Published: May 2022

Abstract
Pasur river is one of the largest rivers in the World Heritage Sundarbans mangrove forest region of the southwestern part of Bangladesh. Due to lack of alternative sources, more than 1 million inhabitants living in the Pasur river basin area rely heavily on the river water for domestic, irrigation, and industrial purposes without proper and reliable information on the water qualities and contamination types. The study aimed at evaluating the suitability and sustainability for irrigation and consumption practices, and suitable hydrogeochemical techniques and quality of Pasur river water of Sundarbon region of Bangladesh were investigated. Water samples were collected from six locations during pre-monsoon and post-monsoon seasons and assessed for suitability for drinking and irrigation application. The water quality index (WQI) was calculated to evaluate the suitability for drinking. WQI indicates that the river water samples during both the seasons are safe for drinking in the good category. Sodium percentage (Na%), sodium adsorption ratio (SAR), magnesium hazard (MH), residual sodium carbonate (RSC) were investigated to assess the feasibility for agricultural applications. Most of the indices, such as SAR, Na%, and RSC results recommend that the river water is safe for irrigation. A suggestion is made that MH in river water should be controlled for the use of water in irrigation. United States Salinity Laboratory (USSL) diagram and Wilcox diagram analysis also identified that river water as a usable category for irrigation purposes is feasible during both seasons.
Application of Heterocycles as an Alternative for the Discovery of New Anti-ulcer
Compounds: A Mini-Review
Authors: de Lócio Lucas Linhares , do Nascimento Agnis Pâmela Simões , Santos Mirelly Barbosa , Gomes Joilly Nilce Santana , de Medeiros e Silva Yvnni Maria Sales , Albino Sonaly Lima , dos Santos Vanda Lúcia , de Moura Ricardo Olímpio 
Published: May 2022

ABSTRACT:
A peptic ulcer is a lesion located in the esophagus, stomach, and upper intestine, caused by an imbalance
between acid secretion and the release of protective mucus. This pathology is prevalent in approximately
14% of the world population and is commonly treated with proton pump inhibitors and type 2 histaminergic receptor
antagonists, however, these drugs present concerning side effects that may lead to gastric cancer. In this
sense, this research aimed to present the main heterocyclics studied in recent years. The screening method for
the choice of articles was based on the selection of publications between 2000 and 2021 present in the Science
Direct, Web of Science, Capes, and Scielo databases, by using the descriptors ''new derivatives'', “heterocyclics”
“antiulcerogenic”, “gastroprotective” and “antisecretor”. This research showed that the most used rings in
the development of anti-ulcer drugs were benzimidazole, quinazoline, thiazole, and thiadiazole. The results also
portray several types of modern in silico, in vitro and in vivo assays, as well as the investigation of different
mechanisms of action, with emphasis on proton pump inhibition, type 2 histaminergic receptor blockers, potassium
competitive acid blockers, type E prostaglandin agonism, anti-secretory activity and anti-oxidant action.
Additionally, the review evidenced the presence of the nitrogen atom in the heterocyclic ring as a determinant
of the potential of the compound. This research suggests new alternatives for the treatment of gastric lesions,
which may be more potent and cause fewer side effects than the currently used, and tend to evolve into more
advanced studies in the coming years.
Poly(rC) Binding Protein 1 Represses the Translation of STAT3 through 5'
UTR
Authors: Jia Rong , Li Ziwei , Wang Xiaole 
Published: May 2022

Background:
Signal transducer and activator of transcription 3 (STAT3) is an oncogene and
frequently overexpressed in cancers. However, the regulatory mechanisms of STAT3 expression are
not fully understood. Poly(rC)-binding protein1 (PCBP1) is an RNA-binding protein that regulates
mRNA stability, splicing, and translation. PCBP1 is a tumor suppressor and can inhibit the translation
of several oncogenic genes.

Objective:
We aimed to understand the regulatory mechanisms of STAT3 expression.

Methods:
The 5' UTR or 3’ UTR regions of the human STAT3 gene were inserted upstream or downstream
of the green fluorescent gene (GFP), respectively, which were used as reporter systems to analyze
the inhibitory effects of PCBP1 on the STAT3 gene expression. The deletion and point mutation
in 5' UTR were used to search the essential regulatory sequences of the translation inhibition. The mutations
of PCBP1 protein were analyzed in the cBioPortal online service. The effects of mutated
PCBP1 proteins on STAT3 expression, cancer cell proliferation, and colony formation were analyzed
in oral squamous cell carcinoma (OSCC) cell lines.

Results:
PCBP1 inhibits mRNA translation through a motif in the 5' UTR of STAT3. Moreover, we
found two leucine residues (Leu100 and Leu102) of PCBP1 protein frequently mutated in cancers.
These mutations abolished the inhibition function of PCBP1 on STAT3 translation. Surprisingly, in
contrast to wild-type PCBP1 protein, these mutations can promote the growth and colony formation of
cancer cells.

Conclusion:
Overall, we demonstrate that PCBP1 can inhibit the expression of STAT3 through its 5'
UTR, and two leucine residues of PCBP1 protein are essential for its functions.
Inhibitory Effect of Standardized Extract and Fractions of Nigella sativa
L. on Nystatin Susceptible and Clinically Nystatin Resistant Candida albicans
Authors: Ayatollahi Mousavi Seyyed Amin , Sharififar Fariba , Jahantiq Abolfazl Saravani , Mohamadi Neda 
Published: May 2022

Introduction:
Candidiasis infection is caused by different species of Candida, which are
characterized by host immunologic weakness. Black cumin seeds (Nigella sativa) have shown an inhibitory
effect against Candida albicans. In this work, the inhibitory effect of standardized extract and
different fractions of Nigella sativa seeds has been evaluated on both nystatin-susceptible and resistant
strain of C. albicans.

Materials and Method:
Canadida albicans (NSCA) with ATCC 76645 and nystatin-resistant Candida
albicans (NRCA) were prepared from oral samples of HIV individuals. Total extract and different
fractions of N. sativa were prepared using maceration and sonication methods. Thymoquinone
(TQ) content of the plant was determined by spectrophotometric method. Total extract (TTE) and the
fractions along with TQ were evaluated on NSCA and NRCA by the microdilution method.

Results:
TQ content of the plant was 0.92 ± 0.37g/100g dried extract. The least MIC and MFC (62.5
and 125 μg/ml, respectively) were due to petroleum ether fraction (PEF) against both NSCA and
NRCA, followed by chloroform fraction (CHF) with MIC and MFC of 125 and 250 μg/ml, respectively.
TQ exhibited MIC of 0.78 and 3.12 μg/ml against NSCA and NRCA, stronger than nystatin (MIC
of 2 and 16 μg/ml, respectively). Thymoquinone was detected in the PEF and CHF.

Conclusion:
Considering more inhibitory effects of PEF and CHF than TTE, we can conclude that active
components of the plant belong to non-polar compounds. PEF showed identical inhibitory effects on
NRCA and NSCA, which is a valuable result for finding novel medicaments against NRCA infections.
Compositional Analysis of Essential Oils from Two Mentha Species and in
silico Study on their Major Volatile Constituents against Polycystic Ovary
Syndrome
Authors: Nickavar Bahman , Nickavar Azar 
Published: May 2022

Background:
Polycystic ovarian syndrome (PCOS) is defined by excessive production and/or
secretion of androgenic hormones in women. This disease has a complicated nature, so its control is difficult
and challenging. Therefore, many women use complementary therapies to support medical treatment,
one of which is the consumption of mint plants.

Objective:
This study aimed to characterize the chemical composition of peppermint (Mentha piperita
L.) and spearmint (Mentha spicata L.) oils, to assess the binding of constituents of the oils to the androgen
receptor as well as their pharmacokinetic features.

Methods:
The essential oils were isolated by water distillation and then analyzed using GC-MS and GCFID.
Thereafter, in silico binding studies were performed between the main volatile constituents and human
androgen receptors using Autodock Vina. Besides, the pharmacokinetic properties of the selected
compounds were evaluated using SwissADME.

Results:
GC analyses showed the presence of 19 and 23 constituents out of the total components (accounting
for 94.7% and 97.6%, respectively), with carvone (73.0%), and menthone (33.1%) and menthol
(29.3%) as the major compounds in spearmint and peppermint oils, respectively. Moreover, molecular
docking studies revealed that carvone has the lowest binding energy to the androgen receptor. On the
other hand, all tested compounds finally exhibited favorable pharmacokinetic parameters.

Conclusion:
The present study virtually indicated that the main volatile constituent in the spearmint oil,
i.e., carvone, could probably cause a beneficial effect on PCOS.
Discovery of 1H-indazole-6-amine Derivatives as Anticancer Agents: Simple
But Effective
Authors: Tran Phuong-Thao , Hoang Ngo Xuan , Hoang Van-Hai , Ngo Thien , Y.Vu Thien 
Published: May 2022

Background:
Indazole is a promising structure present in various biological activity compounds;
in particular, many 6-aminoindazole-containing compounds demonstrated anticancer activity. In
our previous research, we discovered some of the 6-aminoindazole derivatives with excellent cytotoxicity
in the human colorectal cancer cell line (HCT116).

Objective:
In this study, a series of 6-substituted amino-1H-indazole derivatives were designed and synthesized
through simple and well-known chemical reactions, which were evaluated for anti-proliferative
activity in four human cancer cell lines.

Method:
The title compounds were designed based on the structures of potential anticancer candidates in
our previous report. The synthesis of 6-aminoindazole derivatives through acetylation and reductive amination
with 6-amininoindazole as the starting material. Sulforhodamin B (SRB) assay was used for in
vitro biological evaluation of synthesized compounds. Various physicochemical properties of them were
predicted by online site Molinspiration.

Results:
Seven out of eight synthesized compounds showed growth inhibitory activity with IC50 values
from 2.9 to 59.0 μM range in all four tested cancer cell lines. Of them, the compound N-(4-fluorobenzyl)-
1H-indazol-6-amine (9f) exhibited a potent anti-proliferative activity, with an IC50 value of 14.3±4.4 μM
in the human colorectal cancer cell (HCT116) and non-cytotoxicity in the normal cell (lung fibroblast
cells, MRC5, IC50 >100 μM).

Conclusion:
The bioactivity result and conformance of the physicochemical properties of the synthesized
compounds to the "rule of three" for hit-like compounds suggested that 9f was effective and could be used
as a hit for the development of novel anticancer agents.
A Mini Review on the Chemical and Bio-Medicinal Aspects along with
Energy Storage Applications of Anthraquinone and its Analogues
Authors: Shafiq Nusrat , Zareen Gul , Arshad Uzma , Imtiaz Fazeelat , Rashid Maryam , Parveen Shagufta , Yasmin Farah , Ali Basharat 
Published: May 2022

Abstract:
Quinones have established enthusiasm of the researchers because of their enormous applicable
properties. Researchers are now concerned about the synthesized and natural anthraquinone
based derivatives due to their distinctive properties. Intensive research has been made with special
focus on laxative, antiulcerogenic, inhibition of enzymes, cytotoxicity, antimicrobial, antiinflammatory,
antiarthritic, antirheumatic, antidepressant, fungicidal, insecticidal, antioxidant and antitumor
activity of anthraquinone based derivatives. The awareness of environmental protection, safety
and health, free of pollution, disease and particularly to maintain green, are the derivatives being
considered and acknowledged in medicinal research as well as in pharmaceutical world. Anthraquinone
linked with amino derivatives has wide-spectrum therapeutic applications and a center of focus
as an important biological scaffold in different fields like medicines, electronic industry, and cosmetics.
Owing to the remarkable activities of anthraquinone, we outline the structural data, their sources,
synthetic routes and therapeutic potentials.
In-stent Thrombosis and COVID-19 Infection: Current Insights on the
Mechanistic Relationship
Authors: Felekos Ioannis , El-Medany Ahmed , Kandoole Vanessa , Lonsdale Nicholas , Doolub Gemina 
Published: May 2022

:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been
demonstrated as a major risk factor in inducing coronary stent thrombosis due to its propensity to
create a pro-thrombotic state. This review explores the mechanisms that may contribute to the increased
thrombosis risk seen in COVID-19. Furthermore, we discuss the patient and haematological
factors that predispose to an increased risk of stent thrombosis, as well as the role of certain antiplatelet
and anticoagulation therapies, including ticagrelor and enoxaparin, that may reduce the
likelihood and severity of in-stent thrombosis, in SARS-CoV-2 infection. To counter the proinflammatory
and pro-thrombotic state shown in COVID-19, anti-thrombotic therapy in the future
may be optimised using point-of-care platelet inhibition testing and inflammation-modifying therapies.
Large-scale randomised trials with long-term follow-up are increasingly necessary to assess
the intersection of COVID-19 and stent optimisation as well as the reduction of stent thrombosis
after drug-eluting stent (DES) implantation.
Exploring the Anticancer Potentials of Polyphenols: A Comprehensive
Review of Patents in the Last Five Years
Authors: Gupta Nitin , Singh Shaloo , Chauhan Divya , Srivastava Ruchi , Singh Vipendra Kumar 
Published: May 2022

Background:
Polyphenols found abundantly in plants exhibit various anti-carcinogenic effects on tumor cells, including angiogenesis, metastasis, anti-proliferating agents, inflammation, and apoptosis. In recent years, many novel polyphenolic compounds with anticancer activity have been identified worldwide, and few of them are promising anticancer drugs to cure or inhibit cancer growth by interfering with cancer initiation, promotion, and progression.

Objective:
This mini-review aims to provide a comprehensive survey of the information about poly-phenolic anticancer drugs disclosed in worldwide patents and discuss their possibility of developing as drugs used as anticancer drugs in clinical settings.

Methods:
In the present mini-review, we have revealed the anticancer properties of polyphenols pre-sented according to their mechanisms of action. PubMed, Google Patents, and SciDirect databases were used to compile the present study.

Results:
In the last five years, various anticancer polyphenols were revealed in worldwide patents in the last decades, and their mode of action pointed out cytoskeletal damage, arresting cell cycle, in-hibiting kinase, and tumor suppressor protein expression.

Conclusion:
Many newly found polyphenols display a promising anticancer potential both in vitro and in vivo, and a few anticancer polyphenols act to inhibit the growth of various human cancer cells. Also, we have given an overview of patents filed in the last five years related to the anticancer poten-tials of polyphenols.