bluuebunny/crossref_metadata_2025_split huggingface dataset:
The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation
Authors: Lunde Hanne Elisabeth , Hjelmtvedt Agnete Nyborg , Amundsen Erik Koldberg 
Published: June 2022

AbstractIntroductionThe aim of this study was to investigate the diagnostic accuracy of the flag PLT‐Clumps from the WNR/WDF and the PLT‐F channels from Sysmex XN and to study how different cut‐offs for investigation for pseudothrombocytopenia (PTCP) and the definition of platelet aggregation affected the diagnostic accuracy.MethodsA smear review was performed for samples with platelet count <150 × 109/L and samples flagged for platelet aggregation by Sysmex XN‐20. The samples were investigated for platelet aggregation in 30 fields using 40× objective. Findings were classified by size and quantity using two definitions of aggregation: the Norwegian quality improvement of laboratory investigations (Noklus) and the Groupe Francophone de'Hematologie Cellulaire (GFHC). The Q‐values for the PLT‐Clumps flag from the WNR/WFD channel and the PLT‐F channel were compared with smear findings.ResultsROC analysis showed that the diagnostic accuracy of the PLT‐clumps flags increased with increasing stringency of the definition of platelet aggregation and when only samples with thrombocytopenia were investigated. With the most stringent PTCP definitions, the diagnostic accuracy of the PLT‐Clumps flag from PLT‐F was very high (AUC 0.97–0.98) and markedly better than for WNR/WDF.ConclusionThe diagnostic accuracy of PLT‐F from Sysmex XN‐20 for identification of PTCP was very good and superior to the WNDR/WDF channel in samples with platelet count <150 × 109/L and a moderate to high number of aggregates in the smear. There is a need for a more precise definition of platelet aggregation.
Association between maternal antibiotic exposure during pregnancy and childhood obesity in the Japan Environment and Children's Study
Authors: Sakurai Kenichi , Yamamoto Midori , Eguchi Akifumi , Takatani Rieko , Watanabe Masahiro , Mori Chisato ,   the Japan Environment and Children's Study Group
Published: June 2022

SummaryBackgroundThe association between maternal antibiotic exposure during pregnancy and childhood obesity is still unclear.ObjectivesThe study aimed to evaluate the association between prenatal exposure to antibiotics and obesity at age 3 years using data from a large Japanese birth cohort.MethodsThe Japan Environment and Children's Study is a nationwide birth cohort study. In this study, singleton vaginal full‐term births were included. Obesity was defined as body mass index ≥95th percentile according to child growth standards. Prenatal antibiotic exposure was defined as antimicrobial agent use during pregnancy and was collected from maternal interviews and medical record transcripts. Logistic regression analysis was performed to evaluate the association of prenatal antibiotic exposure with child obesity at 3 years.ResultsIn the crude and adjusted models with all children, maternal antibiotic exposure during pregnancy showed a marginal relationship with child obesity at 3 years. In the analyses according to exposure period and sex, exposure to antibiotics during the second/third trimester was significantly associated with obesity at the age of 3 years in female infants, but not in male infants, although the exposure during the first trimester was not in both sexes.ConclusionMaternal antibiotic exposure during mid/late pregnancy may result in child obesity.
Analysis of a situational judgment test for teamwork as a preselection tool for an assessment center: A construct‐based approach
Authors: Goerke Panja , Maier Julia 
Published: June 2022

AbstractSituational judgment tests (SJTs) constitute an efficient and well‐established method for predicting professional success. However, more information is needed regarding their relationship to applicants' behavior as observed in an assessment center (AC) at the construct level. In the present study, an SJT for teamwork (SJT‐TW) was assessed in terms of its suitability as a preselection tool for ACs in the context of a multistage selection. Data were collected from 276 applicants for ab initio pilot training during their selection process. Results from the regression analyses showed that the SJT‐TW test performance is a significant predictor for the AC result and it contributed more than knowledge, cognitive performance tests, and personality scales thereto. The SJT‐TW also caused significant increments for the prediction of single AC dimensions. Therefore, it was concluded that construct‐based SJTs are useful for the assessment of behavioral constructs and can complement selection processes as a preselection tool.
A protocol for the cultivation and monitoring of ileal gut microbiota surrogates
Authors: Akritidou Theodora , Smet Cindy , Akkermans Simen , Tonti Maria , Williams Jennifer , Van de Wiele Tom , Van Impe Jan F. M. 
Published: June 2022

Abstract

Aims
This research aimed to develop and validate a cultivation and monitoring protocol that is suitable for a surrogate microbial community that accounts for the gut microbiota of the ileum of the small intestine.

Methods and Results
Five bacterial species have been selected as representatives of the ileal gut microbiota and a general anaerobic medium (MS-BHI, as minimally supplemented brain heart infusion) has been constructed and validated against BCCM/LGM recommended and commercial media. Moreover, appropriate selective/differential media have been investigated for monitoring each ileal gut microbiota surrogate. Results showed that MS-BHI was highly efficient in displaying individual and collective behaviour of the ileal gut microbiota species, when compared with other types of media. Likewise, the selective/differential media managed to identify and describe the behaviour of their targeted species.

Conclusions
MS-BHI renders a highly efficient, inexpensive and easy-to-prepare cultivation and enumeration alternative for the surrogate ileal microbiota species. Additionally, the selective/differential media can identify and quantify the bacteria of the surrogate ileal microbial community.

Significance and Impact of Study
The selected gut microbiota species can represent an in vitro ileal community, forming the basis for future studies on small intestinal microbiota. MS-BHI and the proposed monitoring protocol can be used as a standard for gut microbiota studies that utilize conventional microbiological techniques.
Potential probiotic strains with heavy metals and mycotoxins bioremoval capacity for application in foodstuffs
Authors: Massoud Ramona , Zoghi Alaleh 
Published: June 2022

Abstract
Heavy metals and mycotoxins in foodstuffs are one of the major concerns of our world nowadays. Food decontamination with the help of microbial biomass is a cheap, easy, efficient and green method known as bioremoval. Probiotics are able to reduce the availability of heavy metals and toxins in food products. The purpose of this review is to summarize the probiotics and potential probiotics' interesting role in food bio-decontamination. After a brief glance at the definition of potential probiotic strains with bioremoval ability, LABs (lactic acid bacteria) are described as they are the most important groups of probiotics. After that, the role of the main probiotic and potential probiotic strains (Bacillus, Lactobacillus, Lactococcus, Enterococcus, Bifidobacterium, Pediococcus, Propionibacterium, Streptococcus and Saccharomyces cerevisiae) for heavy metals and mycotoxins bioremoval are described. Additionally, the bioremoval mechanism and the effect of some factors in bioremoval efficiency are explained. Finally, the investigations about probiotic and contaminant stability are mentioned. It is worth mentioning that this review article can be exerted in different food and beverage industries to eliminate the heavy metals and mycotoxins in foodstuffs.
Ontogeny of swimming performance of hatchery‐reared post‐larvae and juvenile fish: a case of two threatened Mediterranean species
Authors: Ducos Salomé , Pugliese Sally , Demolliens Mikaël , Beraud Louisa , Boussard Alizée , Delmas Alban , Agostini Sylvia , Garcia Jessica , Aiello Antoine , Durieux Eric D. H. 
Published: June 2022

AbstractSwimming performance is a well‐established key physiological parameter of fish that is highly linked to their fitness in the wild. In the context of fish restocking purposes, it therefore appears crucial to study this specific behaviour. Here, the authors investigated intra and interspecies differences in the swimming performance of hatchery‐reared post‐larvae and juveniles belonging to two Mediterranean candidate threatened species, the common dentex, Dentex dentex (Sparidae), and the brown meagre, Sciaena umbra (Sciaenidae), with body sizes ranging from 8 to 37 mm total length (TL, from 24 to 58 days post‐hatch). The swimming abilities were estimated through the calculation of their critical swimming speed (Ucrit), their relative Ucrit and their Reynolds number (Re). Two different patterns were observed between D. dentex and S. umbra, showing a different effect of ontogeny on the performance of both species. The relative Ucrit of S. umbra decreased linearly through ontogeny, whereas the relative Ucrit and Ucrit of D. dentex increased linearly through the range of sizes tested. The ontogenetic change in Ucrit of S. umbra occurred in two stages: a first stage of sharp improvement and a second stage of a slow decrease in performance. Both stages were separated by a breakpoint that coincided with the appearance of a refusal to swim behaviour that occurred shortly after the end of metamorphosis and can potentially be associated with the establishment of this species sedentary behaviour. The swimming performance of both species showed ontogenetic differences. Sciaena umbra had the highest relative performance when its body sizes were the smallest, whereas D. dentex showed the highest relative performance when its body sizes were the largest. These results will be linked to future research on both of these species concerning their escape, exploratory and predatory behaviours, and for restocking purposes to draw a more realistic overview of hatchery‐reared juvenile performance. Knowledge of both species’ behavioural and swimming performance through ontogeny is important to consider when using hatchery‐reared fish juveniles for restocking, as size‐at‐release can have a large impact on fish survival and thus on restocking success.
Comparability of skeletal fibulae surfaces generated by different source scanning (dual‐energy CT scan vs. high resolution laser scanning) and 3D geometric morphometric validation
Authors: Pietrobelli Annalisa , Sorrentino Rita , Notariale Veronica , Durante Stefano , Benazzi Stefano , Marchi Damiano , Belcastro Maria Giovanna 
Published: June 2022

AbstractThis work aims to test accuracy and comparability of 3D models of human skeletal fibulae generated by clinical CT and laser scanner virtual acquisitions. Mesh topology, segmentation and smoothing protocols were tested to assess variation among meshes generated with different scanning methods and procedures, and to evaluate meshes‐interchangeability in 3D geometric morphometric analysis. A sample of 13 left human fibulae were scanned separately with Revolution Discovery CT dual energy (0.625 mm resolution) and ARTEC Space Spider 3D structured light laser scanner (0.1 mm resolution). Different segmentation methods, including half‐maximum height (HMH) and MIA‐clustering protocols, were compared to their high‐resolution standard generated with laser‐scanner by calculating topological surface deviations. Different smoothing algorithms were also evaluated, such as Laplacian and Taubin smoothing. A total of 142 semilandmarks were used to capture the shape of both proximal and distal fibular epiphyses. After Generalized Procrustes superimposition, the Procrustes coordinates of the proximal and distal fibular epiphyses were used separately to assess variation due to scanning methods and the operator error. Smoothing algorithms at low iteration do not provide significant variation among reconstructions, but segmentation protocol may influence final mesh quality (0.09–0.24 mm). Mean deviation among CT‐generated meshes that were segmented with MIA‐clustering protocol, and laser scanner‐generated ones, is optimal (0.42 mm, ranging 0.35–0.56 mm). Principal component analysis reveals that homologous samples scanned with the two methods cluster together for both the proximal and distal fibular epiphyses. Similarly, Procrustes ANOVA reveals no shape differences between scanning methods and replicates, and only 1.38–1.43% of shape variation is due to scanning device. Topological similarities support the comparability of CT‐ and laser scanner‐generated meshes and validate its simultaneous use in shape analysis with potential clinical relevance. We precautionarily suggest that dedicated trials should be performed in each study when merging different data sources prior to analyses.
A clinical prediction model to estimate the risk for coarctation of the aorta: From fetal to newborn life
Authors: Wang Hui‐Hui , Wang Xi‐Ming , Zhu Mei , Liang Hao , Feng Juan , Zhang Nan , Wang Yue‐Mei , Yu Yong‐Hui , Wang An‐Biao 
Published: June 2022

AbstractAimA prenatal diagnosis of coarctation of the aorta (CoA) is challenging. This study aimed to develop a coarctation probability model incorporating prenatal cardiac sonographic markers to estimate the probability of an antenatal diagnosis of CoA.MethodsWe reviewed 89 fetuses as an investigation cohort with prenatal suspicion for CoA and categorized them into three subgroups: severe CoA: symptomatic CoA and surgery within the first 3 months; mild CoA: surgery within 4 months to 1 year (29); and false‐positive CoA: not requiring surgery (45). Logistic regression was used to create a multiparametric model, and a validation cohort of 86 fetuses with suspected CoA was used to validate the model.ResultsThe prediction model had an optimal criterion >0.25 (sensitivity of 97.7%; specificity of 59.1%), and the area under the receiver operator curve was 0.85. The parameters and their cut‐off values were as follows: left common carotid artery to left subclavian artery distance/distal transverse arch (LCCA‐LSCA)/DT Index >1.77 (sensitivity 62%, specificity 88%, 95% confidence interval [CI]: 0.6–0.8), and z‐score of AAo peak Doppler > −1.7 (sensitivity 77%, specificity 56%, 95% CI: 0.6–0.8). The risk assessment demonstrated that fetuses with a model probability >60% should have inpatient observation for a high risk of CoA, whereas fetuses with a model probability <15% should not undergo clinical follow‐up.ConclusionThe probability model performs well in predicting CoA outcomes postnatally and can also improve the accuracy of risk assessment. The objectivity of its parameters may allow its implementation in multicenter studies of fetal cardiology.
The intergenerational transmission of gender: Paternal influences on children's gender attitudes
Authors: Cano Tomás , Hofmeister Heather 
Published: June 2022

AbstractObjectiveThis study provides the first systematic longitudinal analysis of the influence of paternal involvement in family life—across childhood and adolescence—on the gender‐role attitudes of children by the age of 14 or 15.BackgroundRecent research suggests that, in post‐industrial societies, paternal involvement in family life is increasing. Although previous studies of paternal involvement have considered paternal influences on children's cognitive or socio‐emotional development, such studies have not yet addressed paternal influences on children's attitudes toward gender. Relatedly, previous studies on the intergenerational transmission of gender attitudes have analyzed maternal influences, but have neglected the significance of paternal influences. This study engages both strands of the research by analyzing the effects of paternal behaviors on children's attitudes toward gender roles.MethodMultivariate linear regressions models were estimated on data from the Longitudinal Study of Australian Children (LSAC); a survey with biannual observations over 10 years for 2796 children born between 1999 and 2000.ResultsFathers' time spent on childcare during childhood was associated with gender‐egalitarian attitudes in children by the age of 14 or 15. The most powerful predictor of children's gender‐role attitudes, however, was the amount of time fathers spent on housework during children's adolescence, both absolute and relative to the amount of time mothers spent on housework. Fathers' unpaid labor at home was as relevant for children's gender‐role attitudes as mothers' paid labor in the workforce. These results held after controlling for maternal domestic behaviors and for the gender‐role attitudes of both parents.ConclusionFather involvement in childcare and housework during childhood and adolescence play an important role in shaping children's gender‐egalitarian attitudes.
Heritage, the power of the past, and the politics of (mis)recognition
Authors: Smith Laurajane 
Published: June 2022

AbstractHeritage sites and places are often mobilized to represent a group's identity and sense of place and belonging. This paper will illustrate how heritage and museum visiting, as a leisure activity, facilitates or impedes recognition and redistribution in direct and indirect ways. Drawing on extensive qualitative interviews with visitors to 45 heritage sites and museums in the USA, Australia, and England, the paper demonstrates the importance of emotions in mundane struggles over recognition and misrecognition. How emotions uphold or challenge investments in heritage narratives are examined. The paper argues that heritage and heritage‐making is a valuable focus of analysis that reveals the nuances of how people sustain or impede claims for recognition and redistribution.
Mobility Imaginaries of Humanitarian Intervention: Gender, Migration, and Violence along Mexico's Southern Border
Authors: Wurtz Heather M. 
Published: June 2022

AbstractThousands of Central American women have been displaced from their countries of origin by violence. While the violence committed against them is often portrayed as isolated acts of aggression, women's suffering is also produced and perpetuated by humanitarian interventions that immobilize women in dangerous transit zones. Interventions are then justified by institutional logics that juxtapose women's vulnerability against the threat of their own mobility. This article draws on 14 months of ethnographic fieldwork along the southern Mexico border among migrant women who sought out humanitarian assistance following violent encounters. Central to my argument is the concept of mobility imaginaries, or widely shared social assumptions about how mobility should and can be accessed, by whom, and under what circumstances. Through this framework, I show how gendered mobility biases that underlie institutional logics compound other forms of institutional inequality, which often serves to reproduce, rather than mitigate, root causes of gender‐based vulnerability.
Natural variation in root suberization is associated with local environment in Arabidopsis thaliana
Authors: Feng Tao , Wu Pan , Gao Huani , Kosma Dylan K. , Jenks Matthew A. , Lü Shiyou 
Published: June 2022

Summary

Genetic signature of climate adaptation has been widely recognized across the genome of many organisms; however, the eco‐physiological basis for linking genomic polymorphisms with local adaptations remains largely unexplored.

Using a panel of 218 world‐wide Arabidopsis accessions, we characterized the natural variation in root suberization by quantifying 16 suberin monomers. We explored the associations between suberization traits and 126 climate variables. We conducted genome‐wide association analysis and integrated previous genotype–environment association (GEA) to identify the genetic bases underlying suberization variation and their involvements in climate adaptation.

Root suberin content displays extensive variation across Arabidopsis populations and significantly correlates with local moisture gradients and soil characteristics. Specifically, enhanced suberization is associated with drier environments, higher soil cation‐exchange capacity, and lower soil pH; higher proportional levels of very‐long‐chain suberin is negatively correlated with moisture availability, lower soil gravel content, and higher soil silt fraction. We identified 94 putative causal loci and experimentally proved that GPAT6 is involved in C16 suberin biosynthesis. Highly significant associations between the putative genes and environmental variables were observed.

Roots appear highly responsive to environmental heterogeneity via regulation of suberization, especially the suberin composition. The patterns of suberization–environment correlation and the suberin‐related GEA fit the expectations of local adaptation for the polygenic suberization trait.
GmNAC181 promotes symbiotic nodulation and salt tolerance of nodulation by directly regulating GmNINa expression in soybean
Authors: Wang Xiaodi , Chen Kuan , Zhou Miaomiao , Gao Yongkang , Huang Huimei , Liu Chao , Fan Yuanyuan , Fan Zihui , Wang Youning , Li Xia 
Published: June 2022

Summary

Soybean (Glycine max) is one of the most important crops world‐wide. Under low nitrogen (N) condition, soybean can form a symbiotic relationship with rhizobia to acquire sufficient N for their growth and production.

Nodulation signaling controls soybean symbiosis with rhizobia. The soybean Nodule Inception (GmNINa) gene is a central regulator of soybean nodulation. However, the transcriptional regulation of GmNINa remains largely unknown. Nodulation is sensitive to salt stress, but the underlying mechanisms are unclear.

Here, we identified an NAC transcription factor designated GmNAC181 (also known as GmNAC11) as the interacting protein of GmNSP1a. GmNAC181 overexpression or knockdown in soybean resulted in increased or decreased numbers of nodules, respectively. Accordingly, the expression of GmNINa was greatly up‑ and downregulated, respectively. Furthermore, we showed that GmNAC181 can directly bind to the GmNINa promoter to activate its gene expression. Intriguingly, GmNAC181 was highly induced by salt stress during nodulation and promoted symbiotic nodulation under salt stress.

We identified a new transcriptional activator of GmNINa in the nodulation pathway and revealed a mechanism by which GmNAC181 acts as a network node orchestrating the expression of GmNINa and symbiotic nodulation under salt stress conditions.
Bias towards the future
Authors: Greene Preston , Latham Andrew J. , Miller Kristie , Norton James , Tarsney Christian , Tierney Hannah 
Published: June 2022

AbstractAll else being equal, most of us typically prefer to have positive experiences in the future rather than the past and negative experiences in the past rather than the future. Recent empirical evidence tends not only to support the idea that people have these preferences, but further, that people tend to prefer more painful experiences in their past rather than fewer in their future (and mutatis mutandis for pleasant experiences). Are such preferences rationally permissible, or are they, as time‐neutralists contend, rationally impermissible? And what is it that grounds their having the normative status that they do have? We consider two sorts of arguments regarding the normative status of future‐biased preferences. The first appeals to the supposed arbitrariness of these preferences, and the second appeals to their upshot. We evaluate these arguments in light of the recent empirical research on future‐bias.