bluuebunny/crossref_metadata_2025_split huggingface dataset:
Large excess capacity of glycolytic enzymes in Saccharomyces cerevisiae under glucose-limited conditions
Authors: Grigaitis Pranas , Teusink Bas 
Published: July 2022

AbstractIn Nature, microbes live in very nutrient-dynamic environments. Rapid scavenging and consumption of newly introduced nutrients therefore offer a way to outcompete competitors. This may explain the observation that many microorganisms, including the budding yeast Saccharomyces cerevisiae, appear to keep “excess” glycolytic proteins at low growth rates, i.e. the maximal capacity of glycolytic enzymes (largely) exceeds the actual flux through the enzymes. However, such a strategy requires investment into preparatory protein expression that may come at the cost of current fitness. Moreover, at low nutrient levels, enzymes cannot operate at high saturation, and overcapacity is poorly defined without taking enzyme kinetics into account.Here we use computational modeling to suggest that in yeast the overcapacity of the glycolytic enzymes at low specific growth rates is a genuine excess, rather than the optimal enzyme demand dictated by enzyme kinetics. We found that the observed expression of the glycolytic enzymes did match the predicted optimal expression when S. cerevisiae exhibits mixed respiro-fermentative growth, while the expression of tricarboxylic acid cycle enzymes always follows the demand. Moreover, we compared the predicted metabolite concentrations with the experimental measurements and found the best agreement in glucose-excess conditions. We argue that the excess capacity of glycolytic proteins in glucose-scarce conditions is an adaptation of S. cerevisiae to fluctuations of nutrient availability in the environment.
Mobile stamens enhance pollen dispersal by scaring floral visitors away
Authors: Li Deng-Fei , Han Wen-Long , Renner Susanne S. , Huang Shuang-Quan 
Published: July 2022

SummaryAnimal-pollinated plants have to get pollen to a conspecific stigma while protecting it from getting eaten. We provide experimental evidence that touch-sensitive stamens function in (i) enhancing pollen export and (ii) reducing pollen loss to thieves. Stamens of Berberis and Mahonia are inserted between paired nectar glands and when touched by an insect’s tongue rapidly snap forward so that their valvate anthers press pollen on the insect’s tongue or face. We immobilized the stamens in otherwise unmodified flowers and studied pollen transfer in the field and under enclosed conditions. On flowers with immobilized stamens, the commonest bee visitor stayed up to 3.6x longer, yet removed 1.3x fewer pollen grains and deposited 2.1x fewer grains on stigmas per visit. Self-pollen from a single stamen hitting the stigma amounted to 6% of the grains received from single bee visits. Bees discarded pollen passively placed on their bodies, likely because of its berberine content; nectar has no berberine. Syrphid flies fed on both nectar and pollen, taking more when stamens were immobilized. Pollen-tracking experiments in two species showed that mobile-stamen-flowers donate pollen to many more recipients. These results demonstrate another mechanism by which plants simultaneously meter out their pollen and reduce pollen theft.HighlightsStamens that snap forward when triggered by a flower visitor may serve to meter out pollen, scare away pollen thieves, or place pollen more accurately.We tested these hypotheses by experimentally immobilizing all six stamens in numerous flowers of Berberis and Mahonia species in the field and under enclosed conditions.In flowers with immobilized stamens, the commonest bee species stayed up to 3.6x longer, yet removed 1.3x fewer pollen grains and deposited 2.1x fewer grains on stigmas per visit. Mobile stamens exported their pollen to significantly more neighboring flowers.Graphic abstractBehaviour and pollen transfer after flower visitors received a beating on the tongue or in the face by the forward-snapping stamens of Berberis. Stamens only snap forward if their filament basis is touched by an insect tongue.
Solanum lycopersicum CLASS-II KNOXgenes regulate fruit anatomy via gibberellin-dependent and independent pathways
Authors: Shtern Amit , Keren-Keiserman Alexandra , Mauxion Jean-Philippe , Furumizu Chihiro , Alvarez John Paul , Amsellem Ziva , Gil Naama , Motenko Etel , Alkalai-Tuvia Sharon , Fallik Elazar , Gonzalez Nathalie , Goldshmidt Alexander 
Published: July 2022

AbstractThe pericarp is the predominant tissue determining the structural characteristics of most fruits. However, the molecular and genetic mechanisms controlling pericarp development remain only partially understood. Previous studies have identified that CLASS-II KNOX genes regulate fruit size, shape, and maturation inArabidopsis thalianaandSolanum lycopersicum. Here we characterized the roles of theSolanum lycopersicumCLASS-II KNOX (TKN-II) genes in pericarp development via a detailed histological, anatomical, and karyotype analysis of theTKN-IIknockdown (35S:amiR-TKN-II) fruits. We identify that35S:amiR-TKN-IIpericarps contain more cells around their equatorial perimeter and fewer cell layers than the control. In addition, the cell sizes but not the ploidy levels of these pericarps were dramatically reduced.Further, we demonstrate that fruit shape and pericarp layer number phenotypes of the35S:amiR-TKN-IIfruits can be overridden by theproceramutant, known to induce a constitutive response to the plant hormone gibberellin. However, neither theproceramutation nor exogenous gibberellin application can fully rescue the reduced pericarp width and cell size phenotype of35S:amiR-TKN-IIpericarps. Our findings establish that TKN-II genes regulate tomato fruit anatomy, acting via gibberellin to control fruit shape but utilizing a gibberellin-independent pathway to control the size of pericarp cells.HighlightTomatoCLASS-II KNOXgenes regulate fruit and pericarp anatomy via GA-dependent and independent pathways.
Trans-species microRNA loci in the parasitic plant Cuscuta campestris have a U6-like snRNA promoter
Authors: Hudzik Collin , Maguire Sean , Guan Shengxi , Held Jeremy , Axtell Michael J. 
Published: July 2022

AbstractSmall regulatory RNAs can move between organisms during pathogenic interactions and regulate gene expression in the recipient. If and how such “trans-species” small RNAs are distinguished from normal small RNAs is not known. The parasitic plant Cuscuta campestris produces a number of microRNAs that specifically accumulate at the interface between parasite and host, several of which have been demonstrated to have trans-species activity. We find that induction of C. campestris interface-induced microRNAs was similar regardless of host species, and can be replicated in haustoria stimulated to develop in the complete absence of a host. We also find that the loci encoding C. campestris interface-induced microRNAs are distinguished by a common 10 base-pair cis-regulatory element. This element is identical to a previously described upstream sequence element used by all plant small nuclear RNA loci. The sequence context of this element strongly suggests U6-like transcription by RNA polymerase III. The element promotes accumulation of interface-induced miRNAs in a heterologous system. This common promoter element distinguishes C. campestris interface-induced microRNA loci from other plant small RNAs; other plant small RNA loci are transcribed by polymerases II or IV, and lack any common promoter motifs. Our data suggest that C. campestris interface-induced miRNAs are produced in a manner distinct from canonical miRNAs. All confirmed C. campestris microRNAs with confirmed trans-species activity are interface-induced and possess these features. We speculate that this distinct production may allow these miRNAs to be exported to hosts.
Molecular basis for the role of disulfide-linked αCTs in the activation of insulin-like growth factor 1 receptor and insulin receptor
Authors: Li Jie , Wu Jiayi , Bai Xiao-chen , Choi Eunhee 
Published: July 2022

AbstractThe insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1R) control metabolic homeostasis and cell growth and proliferation. The IR and IGF1R form similar disulfide bonds linked homodimers in the apo-state; however, their ligand binding properties and the structures in the active state differ substantially. It has been proposed that the disulfide-linked C-terminal segment of α-chain (αCTs) of the IR and IGF1R control the cooperativity of ligand binding and regulate the receptor activation. Nevertheless, the molecular basis for the roles of disulfide-linked αCTs in IR and IGF1R activation are still unclear. Here, we report the cryo-EM structures of full-length mouse IGF1R/IGF1 and IR/insulin complexes with modified αCTs that have increased flexibility. Unlike the Γ-shaped asymmetric IGF1R dimer with a single IGF1 bound, the IGF1R with the enhanced flexibility of αCTs can form a T-shaped symmetric dimer with two IGF1s bound. Meanwhile, the IR with non-covalently linked αCTs predominantly adopts an asymmetric conformation with four insulins bound, which is distinct from the T-shaped symmetric IR. Using cell-based experiments, we further showed that both IGF1R and IR with the modified αCTs cannot activate the downstream signaling potently. Collectively, our studies demonstrate that the certain structural rigidity of disulfide-linked αCTs is critical for optimal IR and IGF1R signaling activation.
The 5’-3’ exoribonuclease XRN4 modulates the plant circadian network in Arabidopsis
Authors: Careno Daniel A. , Santangelo Soledad Perez , Macknight Richard C. , Yanovsky Marcelo J. 
Published: July 2022

AbstractCircadian rhythms enable organisms to anticipate and adjust their physiology to periodic environmental changes. These rhythms are controlled by biological clocks that consist of a set of clock genes that regulate each other expression. Circadian oscillations in mRNA levels require regulation of mRNA production and degradation. While transcription factors controlling clock function have been well characterized from cyanobacteria to humans, the role of factors controlling mRNA decay is largely unknown. Here, we show that mutations in XRN4, the central component of the 5’-3’ mRNA decay pathway, alter clock function in Arabidopsis. We found that xrn4 mutants display long period phenotypes for clock gene expression and for the rhythm of leaf movement. These circadian defects were associated with changes in the circadian phases, but not overall mRNA levels, of several core clock genes. We then used non-invasive transcriptome-wide mRNA stability analysis to identify genes and pathways regulated by XRN4. Among genes affected in the xrn4 mutant at the transcriptional and post-transcriptional level, we found an enrichment in genes involved in auxin, ethylene, ABA signaling, and also circadian rhythmicity, although no significant effects were observed for canonical core-clock genes. Strikingly, the mRNAs of several clock regulated BBX genes were stabilized in xrn4 mutants. Some of these BBX genes are auxiliary factors controlling the pace of the clock and are candidates to mediate XRN4 effects on circadian period. Our results establish that, in Arabidopsis, the control of 5’-3’ mRNA decay by XRN4 constitutes a novel post-transcriptional regulatory layer of the circadian gene network.
IBD-based estimation of X chromosome effective population size with application to sex-specific demographic history
Authors: Cai Ruoyi , Browning Brian L. , Browning Sharon R. 
Published: July 2022

AbstractThe effective size of a population (Ne) in the recent past can be estimated through analysis of identity-by-descent (IBD) segments. Several methods have been developed for estimatingNefrom autosomal IBD segments, but no such effort has been made with X chromosome IBD segments. In this work, we propose a method to estimate the X chromosome effective population size from X chromosome IBD segments. We show how to use the estimated autosomeNeand X chromosomeNeto estimate female and male effective population sizes. We demonstrate the accuracy of our autosome and X chromosomeNeestimation with simulated data. We find that estimated female and male effective population sizes generally reflect the simulated sex-specific effective population sizes across the past 100 generations, but that short-term differences between the estimated sex-specificNeacross tens of generations may not reliably indicate true sex-specific differences. We analyzed the effective size of populations represented by samples of sequenced UK White British and UK Indian individuals from the UK Biobank.
The effect of brain functional network following electroconvulsive therapy in major depressive disorder
Authors: Tian Shuxiang , Xu Guizhi , Yang Huilan , Fitzgerald Paul B. 
Published: July 2022

Purpose
The purpose of this paper is to examine the changes of brain functional network after electroconvulsive therapy (ECT) treatment in major depressive disorder (MDD).

Design/methodology/approach
In this study, resting electroencephalography (EEG) is used to explore the changes in spectral power density, functional connectivity and network topology elicited by an acute open-label course of ECT in a group of 19 MDD subjects. The brain functional network based on Pearson correlation is constructed in a continuous threshold space (0.38–0.59). Complex network theory is used to analyze the network characteristic such as the length of the characteristic path, clustering coefficient, degree, betweenness centrality, global efficiency and small-world architecture.

Findings
The results show that ECT increased the spectral power density of Delta, Theta and Alpha1 bands and the full frequency. ECT increases the functional connectivity in Delta and full frequency and reduces the functional connectivity in Alpha2 band. In the selected threshold space, the clustering coefficient, global efficiency and small-world attributes of the network are changed significantly after ECT.

Originality/value
The findings indicate that resting EEG could effectively characterize the changes of brain functional networks following ECT in MDD. The results provide a theoretical basis to explore the neurophysiological mechanism of ECT in the field of MDD treatment.
Dramatic social change (COVID-19) moderating complexity leadership and organisational adaptability in Zimbabwean SMEs
Authors: Chingwena Tongesai , Scheepers Caren Brenda 
Published: July 2022

PurposeMajor social changes, such as those induced by the COVID-19 pandemic, intensify the need for organisations in Africa to accelerate adaptation. Leadership plays an important role in their organisations’ adaptation. This study focuses on how leaders can build adaptive organisations through appropriate complexity leadership practices by establishing which of these most predict organisational adaptation. The study aims to contribute to dramatic social change (DSC) theory and to empirically confirm conceptual relationships between complexity leadership theory and perceptions of organisational adaptability (OA).Design/methodology/approachThe convenience non-probability sample include 126 senior management respondents from 24 small and medium enterprises in Zimbabwe. The study focuses on these individual senior managers’ perceptions of their organisations’ adaptation, leadership practices and the social changes during COVID-19. The questionnaire used a five-point Likert scale, based on some items from existing scales on entrepreneurial, operational and enabling leadership of complexity leadership and items on OA and DSC. The study applied structural equation modelling using SmartPLS and SPSS software.FindingsThe study formulates recommendations for the boundary conditions under which each or a combination of the complexity leadership practices will bring about the appropriate level of adaptability. The enabling and entrepreneurial leadership practices required, include brokering, decentralisation and establishing multilevel collaboration.Originality/valueThe study contributes insight for leaders to differentiate between the levels of adaptation their organisations require at particular times in particular contexts. Different adaptations will require a different combination of complexity leadership practices. When the adaptation sought is internal, operational leadership is more appropriate, whereas if the motive is market adaptation, entrepreneurial leadership is more appropriate.
Education for sustainable development through research-based learning in an online environment
Authors: Ahel Oliver , Schirmer Moritz 
Published: July 2022

PurposeThe society faces growing global challenges in terms of sustainable development. A key factor in preparing society for these challenges is education for sustainable development (ESD). This study aims to discuss how the combination of research-based learning (RBL) and digitalization can be used for successful ESD in higher education. The paper introduces the conceptual development of technical and didactical implications for an online learning format, which combines the opportunities of RBL and digitalization to facilitate the distribution of ESD. Furthermore, corresponding evaluation results are presented.Design/methodology/approachA framework, based on key dimensions described by the relevant literature, was applied for the empirical approach to evaluate students’ learning experiences. On this basis, the differences in the students’ learning experiences between the RBL-format and conventional digital learning format (self-directed studies, learning videos and electronic examination) were measured.FindingsThe performed two-samplet-test indicates statistical significant differences in all dimensions. On average, students (N = 226) using the RBL-based format report higher ratings for each examined dimension than students who experienced a conventional format. In summary, this indicates an overall corresponding tendency in students’ learning experience between the examined learning formats.Originality/valueThe presented examination offers empirically based insights on how a digital format can combine RBL and ESD to enhance students’ learning experiences. Moreover, theoretical contributions are provided regarding the future development of digital learning formats to further integrate ESD in higher education.
Achieving organizational resilience through complex adaptive systems approach: a conceptual framework
Authors: Akpinar Hatice , Özer-Çaylan Didem 
Published: July 2022

Purpose
The global business environment causes different and difficult kinds of problems that threaten organizational survival. As an important solution, the organizational resilience concept arises and offers holistic philosophy. The resilience concept offers multidisciplinary and valuable eclectic literature to organizational studies that help to produce a wide variety of solutions but causes a lack of consensus to measure and apply resilience at an organizational level. To fill this gap, this paper aims to offer the complex adaptive systems (CAS) approach as a lens to organizations. This paper aims to offer that CAS provides a suitable toolkit to address the organizational resilience concept as it has the potential to offer more generalizable guidance.

Design/methodology/approach
To reach that goal, this research follows a two-phase systematic literature review. In the first phase, the purpose is to search past five years’ studies (2015–2020) to figure out current trends in organizational resilience concepts. In the second phase, organizational resilience studies, which include the CAS approach, are checked to analyze the alignment procedures of two concepts.

Findings
Literature shows that the organizational resilience concept has rarely worked from the CAS lens.CAS are more resilient via adaptation and learning as they depend on local interactions that shape and co-evolve together with their dynamic environment, which helps the emergence of self-organization in an unpredictable future. To achieve organizational resilience, the CAS lens proposes a generalizable and applicable framework to organizational studies.

Research limitations/implications
Future research should also explore the impact of the interrelationship between two concepts; quantitative and qualitative methods should be applied to be able to get empirical results.

Practical implications
CAS lens could be used as a contemporary design idea. The CAS approach enables the learning and adaptation requirement of the organizational resilience concept, which helps to create dynamic capabilities to handle turbulent changes and gain long-lasting competitiveness. Designing organizations’ agents from CAS viewpoint helps to understand and be ready for new trends, such as digitalization or Industry 4.0.

Social implications
Evaluating organizations from the CAS lens as living systems that co-evolve with their environments reveals different ways to achieve organizational resilience management philosophy. Designing organizations as CAS promotes organizational adaptation and learning, which improves organizational decision-making, increasing organizational resilience in organizations.

Originality/value
The originality of the study lies in proposing to achieve organizational resilience via CAS lenses and offering a conceptual framework to achieve organizational resilience.
Survivability versus rate of recovery for skeletal elements in forensic anthropology
Authors: Scott Shelby , Jantz Richard L. 
Published: July 2022

AbstractSurvivability, the ability of a skeletal element to withstand taphonomic processes, is often equated to recoverability, the probability that an element will be recovered in a forensic context, and further misused to infer the likelihood that a forensic anthropologist will recover a particular element at a scene. Consequently, researchers have utilized notions of survivability to infer that a skeletal element may be recovered when justifying the necessity of various research endeavors. This is problematic because the factors impacting survivability are not always applicable in a forensic context; the ability of a bone to survive taphonomic processes may not align with the likelihood of recovery. Empirical recovery rates are presented from two distinct contexts, with data derived from the Forensic Anthropology Data Bank based on cases performed by the late J. Lawrence Angel (1914–1986) and cases done by the University of Tennessee Knoxville (UTK). Recovery rates may be influenced by factors beyond survivability, though we do not investigate the many considerations that might explain recovery rate variation between datasets. Rather, these data exemplify the conceptual differences between notions of survivability and rates of recovery in actual casework scenarios. Thus, it is proposed that researchers consider documented rates of recovery when providing rationale for forensic anthropology research endeavors, in addition to citing a rationale that is based on inferences of survivability. This ensures that the theoretical framework of future forensic anthropology research stems, primarily, from the premise of practical application.