bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE RELATIONSHIP BETWEEN KNOWLEDGE AND DISASTER PREPAREDNESS IN NURSING STUDENTS STIKES YATSI TANGERANG IN 2021
Authors: Octaviana Fanisha , Jaenudin  
Published: November 2022

Introduction: The occurrence of disasters in 2017-2021 reached 12,199 disasters consisting of floods, tornadoes, landslides, forest fires, earthquakes, abrasion, and volcanic eruptions. Many disasters have resulted in 4,851 deaths and 39,633 people missing and injured (BNPB, 2021). Methods: This research is a quantitative correlation study with a cross-sectional approach. The sampling technique used in this study was total sampling, which consisted of 335 respondents from nursing students at STIKes Yatsi, Tangerang. Data collection was done by distributing questionnaires. Data analysis using chi-square test. Results: The results showed that most female students had a good level of knowledge (55,2%) and preparedness (58,5%). The bivariate analysis results showed a significant relationship between knowledge and disaster preparedness r = 0.016 (r < 0.05). The study concluded that the better the student's knowledge, the better the disaster preparedness. Conclusions: The study found a relationship between knowledge and disaster preparedness in students.
NURSING CARE FOR MOTHERS WITH INCOMPLETE ABORTIONS: CASE STUDY
Authors: Fadliyah Lailatul , Puspitaningtyas Danty Indra , Sulpat Emuliana , Ma’rifah Umi 
Published: November 2022

Introduction: Incomplete abortion is bleeding in pregnancy before 20 weeks, where some of the products of conception have come out of the uterine cavity through the cervical canal left in the decidua or placenta. Methods: This research uses a case study design. Data collection from assessment to nursing evaluation was carried out in the jasmine room of Dr Soegiri Lamongan Hospital in February 2019. Participant care at the hospital for three days—data collection techniques using interviews, observation, and documentation. Data analysis uses narrative analysis based on the analysis of relevant facts and theories. Results: The assessment of the two participants was the first and second pregnancies, with gestational ages of 12 and 16 weeks, experiencing vaginal bleeding and abdominal pain. There is abdominal tenderness, a pain scale of 5, and uterine contractions on examination. The nursing diagnosis was acute pain associated with uterine contractions and fluid volume deficit related to bleeding (abortion). The evaluation was carried out for 72 hours of reduced pain. The discussion is expected that nurses provide health education about pain and incomplete abortion. Conclusions: The pain and anxiety were reduced after implementing nursing for 72 hours. The reference from this study is for nurses to provide health education related to pain in incomplete abortion.
Determination of HIV Tropism in Patients with Antiretroviral Therapy Failure in Arkhangelsk Region
Authors: Ostankova Yu. V. , Davydenko V. S. , Shchemelev A. N. , Zueva E. B. , Virolainen P. A. , Totolyan Areg A. 
Published: November 2022

The aim of the study was to determine the tropism of the human immunodeficiency virus in patients with virological failure of antiretroviral therapy (ART) from the Arkhangelsk Region based on the analysis of the env gene V3 loop nucleotide sequence.Materials and methods. We used blood plasma samples obtained from 76 HIV-infected persons from the Arkhangelsk Region with virological failure of antiretroviral therapy. The nucleotide sequences of the HIV env gene C2-V3-C3 region were studied by PCR followed by sequencing. The genotype of the studied strains was determined based on the analysis of their phylogenetic relations with reference sequences from the international GenBank database, as well as using specialized programs. To predict viral tropism, the Garrido rule and the online bioinformatic tool Geno2Pheno[coreceptor] were used. The Geno2Pheno[coreceptor] algorithm, determines the false positive rate (FPR) based on the analysis of the env gene V3 loop nucleotide sequence. Results and discussion. Significantly lower representation of R5X4/X4-tropic HIV variants in long-term infected persons with subsubtype A6 virus compared to subtype B virus has been shown. For all FPR cut-off algorithms, a significant correlation between subtype and HIV tropism was observed (p=0.0014 and p=0.013 for FPR 10 % and FPR 20 %, respectively). While among subtype B strains, at least 57 % were identified as R5X4/X4-tropic variants (for an FPR of 10 %), including two strains classified as X4-tropic; among HIV subsubtype A6 even at an FPR of 20 %, the frequency of R5X4/X4-tropic samples only slightly exceeded 22 %. It can be assumed that the dynamics of changes in HIV tropism depends on the virus subtype. Significant differences in the distribution of amino acid residues of the V3 region sequences in the examined group between R5-tropic and R5X4/X4-tropic strains of subsubtype A6 for positions 18 (χ2=7.616, p=0.0058), 21 (χ2=7.281, p=0.007), 24 (χ2=5.587, p=0.0181), and 34 (χ2=5.144, p=0.0233) have been demonstrated. Among the R5X4/X4-tropic strains of the A6 subsubtype, amino acid substitutions were registered at positions 6, 19, 21, 26, 29, 30, which were not found in the R5-tropic A6 strains. The high occurrence frequency of a number of mutations previously described as presumably associated with resistance to maraviroc and similar drugs may indicate a natural polymorphism characteristic of the A6 subsubtype, which does not correlate with resistance to CCR5 co-receptor antagonists.
Improvement of Approaches to the Verification of the Vaccine Strain <i>Francisella tularensis</i> 15 NIIEG during Long-Term Storage
Authors: Sayapina L. V. , Osina N. A. , Naryshkina E. A. , Fedorov A. V. , Krasnov Ya. M. , Davydov D. S. , Bondarev V. P. 
Published: November 2022

The aim of the study was to improve the methods for verifying the vaccine strain Francisella tularensis 15 NIIEG during long-term storage under current conditions.Materials and methods. The paper summarizes the results of studying the phenotypic and genetic properties of lyophilized cultures of the vaccine strain F. tularensis 15 NIIEG (1953, 1966, 1969, 1987, 1990, 2003, 2012 and 2013) stored at SCEMAP for a period of one to 60 years.Results and discussion. Previous studies have revealed that freeze-dried cultures of F. tularensis 15 NIIEG generally had the characteristics of the vaccine strain, with the exception of deviations from the regulatory requirements for residual virulence and specific safety. The stability of preservation of deletions in the pilA and pilE genes (the region of differentiation RD19) and the genes encoding lpp lipoprotein (RD18) in the vaccine strain, which was stored for various periods of time in a lyophilized state, has been confirmed. The vaccine-strain-specific mutation C178404T (by the genome of F. tularensis LVS strain, GenBank NCBI no. CP009694) has been identified, and an approach to determine it has been developed. The data obtained are promising as regards using the above deletions in the RD18/RD19 regions in combination with the C178404T mutation to assess the authenticity of the vaccine strain using molecular genetic methods. Thus, the conducted retrospective analysis of the data on the cultures of tularemia microbe vaccine strain from the 1940s to 2013 and the gathered experimental data, made it possible to supplement the uniform requirements for the manufacture, study, maintenance, storage and movement of F. tularensis 15 NIIEG vaccine strain with new evidence. Based on the results obtained, the authors have drawn a draft methodological recommendations of the federal level “Vaccinal strain Francisella tularensis 15 NIIEG: order of handling”.
The Effectiveness of MALDI ToF Mass Spectrometry in Identification of <i>Francisella tularensis</i> Strains
Authors: Syngeeva A. K. , Ostyak A. S. , Kulikalova E. S. , Mazepa A. V. , Naumova K. V. , Balakhonov S. V. 
Published: November 2022

The aim of the study was to evaluate the effectiveness of MALDI‑ToF mass spectrometry in the identification of collection and newly isolated strains of tularemia pathogen using the database “Protein profiles of mass spectra of microorganisms belonging to I–II pathogenicity groups for the MALDI Biotyper software”.Materials and methods. We investigated 142 strains of Francisella tularensis, including 59 collection strains and 83 newly isolated ones. Bacteriological, molecular-genetic and proteomic research methods were used to identify them. The acquisition of mass spectra, analysis, generation and expansion of reference libraries were performed on a mass analyzer “Microflex LT” using FlexControl v. 3.3, FlexAnalysis v. 3.3, and MALDI Biotyper 3.0 software packages. The cluster analysis was performed using the BioNumerics 7.6 software.Results and discussion. The possibility of identifying tularemia pathogen has been assessed using the extended database for MALDI Biotyper 3.0 “Protein profiles of mass spectra of microorganisms belonging to I–II pathogenicity groups for the MALDI Biotyper software”. During identification to the species level, the significance of mass spectrometry results for collection strains and newly isolated ones was 91.5 % and 97.6 %, respectively. In determining the genus appurtenance, the reliability of identification was 100 %. Thus, the MALDI‑ToF mass spectrometry method allows for accurate species and genus identification of F. tularensis strains. Based on the cluster analysis of 66 F. tularensis strains in BioNumerics 7.6 software using «Pearson correlation» and the UPGMA algorithm, the possibility of subspecies differentiation has been evaluated. Due to the similarity of protein profiles of F. tularensis strains, a clear differentiation into subspecies could not be achieved. It is necessary to use other options for sample preparation, new generation devices with higher resolution, as well as apply additional approaches and analysis tools for successful subspecific differentiation.
Antibiotic Resistance of Uropathogenic <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections at the Urological Inpatient Facility of the Saratov Clinical Hospital
Authors: Kazantsev A. V. , Proskuryakova M. V. , Kazakova E. S. , Osina N. A. , Shvidenko I. G. , Mikerov A. N. 
Published: November 2022

The aim of the work was to study the profile of antibiotic resistance of uropathogenic Escherichia coli strains isolated from patients with urinary tract infections in the urological inpatient facility of the clinical hospital in the Saratov city, depending on appurtenance to phylogenetic groups and subgroups, as well as O-serogroups.Materials and methods. We assessed sensitivity/resistance to 25 different antibacterial drugs in 102 strains of uropathogenic E. coli. The studies were carried out using the disk diffusion method. The production of extended spectrum beta-lactamases was evaluated by the double disk method. Carbapenemase output was determined using the CIM test. The PCR method was applied to determine appurtenance to phylogenetic groups and subgroups, O-serogroups, as well as the frequency of occurrence of the mcr‑1, mcr‑2, mcr‑3, mcr‑4, mcr‑5 genes encoding the proteins that mediate the development of resistance to colistin.Results and discussion. It has been established that all strains of uropathogenic E. coli are more or less resistant to antibacterial drugs. All studied 102 strains showed resistance to 23 antibacterial drugs from 8 functional groups. The resistance of uropathogenic E. coli had certain differences depending on belonging to phylogenetic groups and subgroups, O-serogroups. Strains of uropathogenic E. coli with high resistance (up to 100 %) belonged to the B23 phylogenetic group, the main representatives of which are cultures of the most common O-25 serogroup. The production of extended-spectrum beta-lactamases has been phenotypically confirmed for 69 (67.6 %) strains. No carbapenemaseproducing cultures were found in the study. The mcr‑1 and mcr‑2 genes encoding resistance to colistin have been identified in 3 uropathogenic E. coli strains (2.9 %).
Designing Novel Hard-Coating Free Erosion-Corrosion Resistant Nano-Composite(s) for Oilfield Applications: From Pump Liners to Washpipe Sleeves
Authors: Roy Ting , Ben Naceur Kamel , Koyanagi Yoshihiko , Furusho Chihiro , Meissner Siggi , Ardic Hifzi , Roy Indranil 
Published: November 2022

Abstract
Rapid frequency and mean time between failures (MTBF), affecting the overall performance of components such as pump liners, entire fluid ends and washpipe sleeves deployed in aggressive & abrasive environments, presents innovation opportunities to mitigate and improve failure intervals. In-depth review of the needs of collaborating end-users, warranted design of Ultra-Hard-Coating free (CVD, PVD, DLC, HVOF etc.), erosion-corrosion resistant nano-composite(s) (CRn-C) for oilfield-applications.
Given our vast experience with Ultra-Hard-Coatings on a softer metal-matrix, wherein dynamic surface force due to impinging hard-particulates like grains of sand enveloped in a fluid (slick-water) creates hydrodynamic-forces acting on the particulates resulting in fracture of Hard- Coatings at a microscopic length-scale, commonly characterized as erosion. Breach of the coating and subsequent entry of corrosive-fluids between the coating and softer metal-substrate causes crevice-corrosion leading to catastrophic failures.
To help bridge this challenging "Technology-gap", here we present the design of nano- composites wherein the base-material is a corrosion resistant alloy (CRA) having multi- nano- phase inclusions, with loadings up-to 90%.
Invisible gold in the Archean detrital sulphides of the Witwatersrand tailings dumps: A large and under-exploited gold resource
Authors: Chingwaru Steve , Heyden Bjorn Von der , Tadie Margreth 
Published: November 2022

Abstract

The tailings dumps originating from gold mining in South Africa’s Witwatersrand still contain notable gold endowments. Most tailings reprocessing operations target a native gold fraction using re-milling and carbon-in-leach extraction; however, up to 50-70% of the remaining gold is still not recoverable and instead reports to a discard stream along with abundant sulphides. The mineralogical deportment of this unrecoverable gold requires detailed investigation. Using in situ laser ablation mineral chemistry measurements, we show that this gold that is ‘invisible’ to conventional recovery is hosted preferentially in pyrite and arsenian pyrite. Importantly, complementary optical and electron microscopies reveal that the rounded detrital forms of these minerals contain the highest gold concentrations (0,01–2730 ppm), showing some similarity to values reported for sulphides from primary orogenic gold deposits found in surrounding Archean-aged granite-greenstone belt remnants. We suggest that detrital auriferous sulphides have been overlooked by historical primary and secondary beneficiation, and thus represent a large (up to 420 tons) and under-exploited Au resource currently residing in easily-mined (surficial) Witwatersrand tailings dumps. We further suggest that targeted re-mining of sulphide mineral fraction has the potential to improve gold recovery, recuperate ‘sweetener’ by-product metals (e.g., Cu, Co, Ni) and directly eliminate heavy metal pollution and acid mine drainage issues associated with surficial tailings dumps.
Preoperative Blood Urea Nitrogen-to-Serum Albumin Ratio for the Prediction of In-hospital Mortality in Patients who Underwent Emergency Surgery for Acute Type A Aortic Dissection
Authors: Wu Qingsong , Lin Jianling , Xie Linfeng , Qiu Zhihuang , Tang Mirong , Chen Liangwan 
Published: November 2022

Abstract

Background
Blood urea nitrogen (BUN) and serum albumin (SA) are related to mortality in patients with cardiovascular diseases, such as acute aortic dissection. However, there are no data on the relationship between BUN-to-albumin ratio (BA-R) and mortality in patients with acute type A aortic dissection (ATAAD). Thus, the aim of this study was to evaluate the prognostic value of BA-R for the prediction of in-hospital mortality in patients who underwent emergency surgery for ATAAD.
Methods
Patients who were diagnosed with ATAAD and underwent emergency surgery within 48 h of onset at our hospital between January 2015 and December 2021 were included in this study. The primary endpoint of this study was postoperative in-hospital mortality (POIM). The data of the survivors and non-survivors were retrospectively compared. Univariate and multivariable logistic regression analyses were performed to investigate the association between BA-R and POIM.
Results
A total of 557 patients with ATAAD were enrolled in this study. Of these, 505 survived and 52 did not. The preoperative BA-R of the non-survivor group was significantly higher than that of the survivor group (0.27 [0.18, 0.46] vs. 0.12 [0.10, 0.16] mmol/g; P < 0.001). Univariate regression analysis showed that preoperative BA-R ≥ 0.155 mmol/g, serum creatinine level ≥ 96.3 µmol/mL, SA level < 37.05 g/L, D-dimer level ≥ 8.21 µg/mL, age ≥ 56 years, myocardial ischemia, cerebral ischemia, and aortic clamp time ≥ 49.6 min were risk factors for POIM. In addition, multivariable regression analysis showed that preoperative BA-R ≥ 0.155 mmol/g was a risk factor for POIM (odds ratio, 6.815 [3.582–12.964]; P < 0.001). Receiver operating characteristic curve indicated that the cut-off point for preoperative BA-R was ≥ 0.155 mmol/g (area under the curve = 0.874). The sensitivity and specificity of preoperative BA-R in predicting the POIM of patients who underwent emergency surgery for ATAAD were 84.6% and 71.3%, respectively (95% confidence interval, 0.829–0.919; P < 0.001).
Conclusion
Preoperative BA-R is a simple, rapid, and potentially useful prognostic indicator of POIM in patients with ATAAD.
Biological properties and antifungal activity of Acalypha wilkesiana, Mitracarpus hirtus, Senna alata on Candida strains isolated from hospital stool samples in Southern Benin
Authors: KLOTOE Jean Robert , FAH Lauris , FANOU Brice Armand , DEGUENON Esther , AGBODJENTO Eric , ASSOGBA Phénix , TOHIO Freed , DOUGNON Victorien , LOKO Frédéric 
Published: November 2022

Abstract
Candidiasis is one of the opportunistic diseases that occur following HIV/AIDS infection. Currently, the management of candidiasis is problematic due to the emergence of resistance to classical antifungal drugs. The search for alternative solutions for the discovery of new bioactive molecules is necessary. This study aims to explore the antifungal properties of Mitracarptus hirtus, Acalypha wikesian and Senna alata on Candida strains isolated from stools of people living with the acquired immunodeficiency virus and collected in some hospitals in Benin and then confirmed by molecular biology techniques at the Research Unit in Microbiology and Pharmacology of natural substances. The strains were tested for different resistance factors. The main phytochemical groups of the plants were searched by staining methods from the dry matter of the studied plants. The determination of total polyphenols and the evaluation of the antioxidant activity of the extracts by the DPPH radical scavenging test were carried out by spectrophotometric methods. The in vitro antifungal activity of the aqueous and hydroethanol extracts of these plants was then evaluated on the strains. The antifungal test data revealed that of the three plants tested, only Mitracarptus hirtus showed inhibitory activity on the growth of the reference strain of Candida albicans ATCC 90028. The hydroethanol extract of this plant was active on all clinical strains with a better effect at the concentration of 100 mg/ml. The data obtained also highlighted the richness in total polyphenols and the anti-free radical activity of the plant extracts studied. This study proved particularly the anti-candidus properties of the hydro-ethanolic extract of the leafy stem of Mitracarptus hirtus. Further studies will generate data on the in vivo efficacy and mechanism of action of the anti-candidosis effect of Mitracarptus hirtus.
Risk Factors of Breast Cancer Determination: a Comparative Study on Different Feature Selection Techniques
Authors: Maalej Zainab , Rejab Fahmi Ben , Nouira Kaouther 
Published: November 2022

Abstract
Purpose: Choosing the relevant features is important to provide a better understanding of the data and improve the prediction performance. Thus, the main aim of this paper is to identify the risk factors of breast cancer.
Methods: focusing on two different datasets: Breast Cancer Surveillance Consortium (BCSC) and Breast Cancer Coimbra (BCC), we perform a comparative study of various feature selection methods: Filter Methods, Wrapper Methods and Embedded Methods. In addition, this work investigates the stability of these techniques when perturbation on datasets is added. Artficial Neural Network, Random Forest, SVM, Logistic Regression and Decision Tree are used for classification. Results: The results are compared when using all the features and when using only the top ranked. The classification performances are comparable in either cases. Furthermore, we found that invasive, glucose, resistin, insulin, leptin, age, adiponectin, BMI and HOMA are the most relevant features that promote breast cancer.
Conclusion: Our findings demonstrate that the identified feature selection methods can efficiently determine the risk factors of breast cancer.
Survey of Plant-Parasitic Nematodes in Jerash Province of Jordan
Authors: Al-ameiri Nofal Suleiman Mohammad 
Published: November 2022

Abstract
Nematodes are devastating of crop plants in agricultural production and certainly contributing significantly to net reduction in crop yield. A general survey to the whole country of Jordan was done and only one comprehensive survey in one province (Karak Province) was previously done. Therefore, this study aimed at conducting a comprehensive survey for plant-parasitic nematodes in Jerash Province in the Northern part of Jordan. Results revealed widespread distribution of different nematode species throughout Jerash province, and only 5.4% of soil samples were not infested with the plant-parasitic nematodes. All percentages of symptoms of cereal hosts were lower than the other hosts. Root galling in vegetables was 68% compared to cereals with only 9%. Several genera of nematodes were identified in this study in which Meloidogyne and Pratylechus were detected almost in all samples collected with 92% for Meloidogyne spp. and 81% for Pratylenchus followed by Helicotylenchus, Xiphinema, and Longidorus. The outcomes of this investigation are relevant by enhancing the basic knowledge of plant parasitic nematodes in Jerash.