bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Homogeneous Time-Resolved Fluorescence (HTRF™) to Monitor Poly-ubiquitination of Wild-type p53
Authors: Yabuki Nami , Watanabe Shin-ichi , Kudoh Tsutomu , Nihira Shin-ichi , Miyamoto Chikara 
Published: February 2022

Abstract:
Rapid degradation of wild-type p53 in the human uterine cervix is induced by the infection of high-risk human papilloma virus (HPV) types 16 and 18. HPV-E6 protein plays a critical role in the poly-ubiquitination of wild-type p53 by mediating the association of p53 with E6-associated protein (E6AP). As a result, the poly-ubiquitinated p53 is rapidly and selectively degraded by the 26S proteasome. We have established a high throughput assay system to monitor poly-ubiquitination of wild-type p53 using a new fluorescence homogeneous technology known as Homogeneous Time-Resolved Fluorescence (HTRF™). The Europium Cryptate (Eu(K)]-Iabeled ubiquitins are incorporated into poly-ubiquitin chains conjugated with the biotinylated p53. In the HTRF assay, Europium cryptate-labeled ubiquitin and streptavidin­ labeled allophycocyanin (XL665) are used as the fluorescence donor and acceptor, respectively. The biotinylated p53 is ubiquitinated by ubiquitination enzymes, then by the addition of streptavidin-labeled XL665, the donor and acceptor molecules are brought in close proximity, thereby generating fluorescent signals. This time-resolved fluorescence assay system shows a sufficient signal for its application in synthetic compound screening and having almost
the same level of sensitivity as that monitored by the scintillation proximity assay (SPA) using 1251-
labeled ubiquitin. The detection of poly-ubiquitination of wild-type p53 by using the HTRF™ or SPA systems described here is much easier and quicker than by using conventional methods. Therefore, these new systems would be appropriate for high throughput screening of compounds for the discovery of new inhibitors of poly-ubiquitination of wild-type p53.
Inhibitors and Activators of the p38 Mitogen-Activated MAP Kinase
(MAPK) Family as Drugs to Treat Cancer and Inflammation
Authors: Denny William A. 
Published: February 2022

Abstract:
The p38 MAP kinases are a sub-family of the broad group of mitogen-activated serinethreonine
protein kinases. The best-characterised, most widely expressed, and most targeted by drugs
is p38α MAP kinase. This review briefly summarises the place of p38α MAP kinase in cellular signalling
and discusses the structures and activity profiles of representative examples of the major classes of
inhibitors and activators (both synthetic compounds and natural products) of this enzyme. Primary
screening was direct in vitro inhibition of isolated p38α enzyme.
The Interactions among Hypertension, Cancer, and COVID-19: Perspective with Regard to Ca2+/cAMP Signalling
Authors: Bergantin Leandro Bueno 
Published: February 2022

Background:
The hypothesis that hypertension is clinically associated with an enhanced
risk of developing cancer has been highlighted. However, the working principles involved in this link
are still under intensive discussion. A correlation among inflammation, hypertension, and cancer could
accurately describe the clinical link between these diseases. In addition, dyshomeostasis of Ca2+ has
been considered to be involved in both cancer and hypertension, and inflammation. There is a strong
link between Ca2+ signalling, e.g. enhanced Ca2+ signals, and inflammatory outcomes. cAMP also
modulates pro- and anti-inflammatory outcomes; pharmaceuticals, which increase intracellular cAMP
levels, can decrease the production of proinflammatory mediators and enhance the production of antiinflammatory
outcomes.

Objective:
This article highlights the participation of Ca2+/cAMP signalling in the clinical association
among inflammation, hypertension, and an enhanced risk for the development of cancer. In addition,
considering that research on coronavirus disease 2019 (COVID-19) is a rapidly evolving field, this
article also reviews recent reports related to the role of Ca2+ channel blockers in restoring Ca2+ signalling
disruption due to COVID-19, including the relationship among COVID-19, cancer, and hypertension.

Conclusion:
An understanding of the association among these diseases could expand current pharmacotherapy,
involving Ca2+ channel blockers and pharmaceuticals that facilitate a rise in cAMP levels.
Consequences of Antipsychotic Medications on Mental Health
Authors: Bukhari Syed Nasir Abbas 
Published: February 2022

Abstract:
Individuals suffering from mental illnesses, unfortunately, have a shorter lifespan. An
increase in mortality rates is primarily due to physical illness, unhealthy lifestyle, and associated
comorbidities. Antipsychotic medications, previously known as tranquilizers, antipsychotics, or
neuroleptics, can alleviate or attenuate symptoms related to psychosis, delusion, and/or hallucinations
and are used in the treatment of psychosis, schizophrenia, bipolar disorder, depression, or
Alzheimer's disease. Within hours to days, these medications alleviate symptoms and reduce confusion
in individuals with psychosis; however, the full effect may take longer than usual in some
cases. Importantly, these drugs do not cure the disease, but only treat the symptoms of the disease.
The treatment is adjusted to reduce any psychotic symptoms while keeping the adverse effects to a
minimum level. Antipsychotics may lead to increased risk of diseases, including but not limited to,
diabetes, obesity, metabolic disorders, cardiovascular, renal, or respiratory disorders. Improved
dosages, polypharmacy, and age-specific treatment play an important role in limiting the comorbidities,
as well as the side effects. Further research and clinical attention are required to understand
the functioning of these medications. The review focuses on the use of antipsychotic medications
in different diseases and their effect on mental health.
Digital and Mobile Health Technology in Collaborative Behavioral Health Care: Scoping Review
Authors: Moon Khatiya , Sobolev Michael , Kane John M 
Published: February 2022

Background
The collaborative care model (CoCM) is a well-established system of behavioral health care in primary care settings. There is potential for digital and mobile technology to augment the CoCM to improve access, scalability, efficiency, and clinical outcomes.

Objective
This study aims to conduct a scoping review to synthesize the evidence available on digital and mobile health technology in collaborative care settings.

Methods
This review included cohort and experimental studies of digital and mobile technologies used to augment the CoCM. Studies examining primary care without collaborative care were excluded. A literature search was conducted using 4 electronic databases (MEDLINE, Embase, Web of Science, and Google Scholar). The search results were screened in 2 stages (title and abstract screening, followed by full-text review) by 2 reviewers.

Results
A total of 3982 nonduplicate reports were identified, of which 20 (0.5%) were included in the analysis. Most studies used a combination of novel technologies. The range of digital and mobile health technologies used included mobile apps, websites, web-based platforms, telephone-based interactive voice recordings, and mobile sensor data. None of the identified studies used social media or wearable devices. Studies that measured patient and provider satisfaction reported positive results, although some types of interventions increased provider workload, and engagement was variable. In studies where clinical outcomes were measured (7/20, 35%), there were no differences between groups, or the differences were modest.

Conclusions
The use of digital and mobile health technologies in CoCM is still limited. This study found that technology was most successful when it was integrated into the existing workflow without relying on patient or provider initiative. However, the effect of digital and mobile health on clinical outcomes in CoCM remains unclear and requires additional clinical trials.
The Content, Quality, and Behavior Change Techniques in Nutrition-Themed Mobile Apps for Children in Canada: App Review and Evaluation Study
Authors: Brown Jacqueline Marie , Franco-Arellano Beatriz , Froome Hannah , Siddiqi Amina , Mahmood Amina , Arcand JoAnne 
Published: February 2022

Background
Children increasingly use mobile apps. Strategies to increase child engagement with apps include the use of gamification and images that incite fun and interaction, such as food. However, the foods and beverages that children are exposed to while using apps are unknown and may vary by app type.

Objective
The aim of this study is to identify the app content (ie, types of foods and beverages) included in nutrition-themed apps intended for children, to assess the use of game-like features, and to examine app characteristics such as overall quality and behavior change techniques (BCTs).

Methods
This analysis used a cross-sectional database of nutrition-themed apps intended for children (≤12 years), collected between May 2018 and June 2019 from the Apple App Store and Google Play Store (n=259). Apps were classified into four types: food games or nongames that included didactic nutrition guides, habit trackers, and other. Food and beverages were identified in apps and classified into 16 food categories, as recommended (8/16, 50%) and as not recommended (8/16, 50%) by dietary guidelines, and quantified by app type. Binomial logistic regression assessed whether game apps were associated with foods and beverages not recommended by guidelines. App quality, overall and by subscales, was determined using the Mobile App Rating Scale. The BCT Taxonomy was used to classify the different behavioral techniques that were identified in a subsample of apps (124/259, 47.9%).

Results
A total of 259 apps displayed a median of 6 (IQR 3) foods and beverages. Moreover, 62.5% (162/259) of apps were classified as food games, 27.4% (71/259) as didactic nutrition guides, 6.6% (17/259) as habit trackers, and 3.5% (9/259) as other. Most apps (198/259, 76.4%) displayed at least one food or beverage that was not recommended by the dietary guidelines. Food game apps were almost 3 times more likely to display food and beverages not recommended by the guidelines compared with nongame apps (β=2.8; P<.001). The overall app quality was moderate, with a median Mobile App Rating Scale score of 3.6 (IQR 0.7). Functionality was the subscale with the highest score (median 4, IQR 0.3). Nutrition guides were more likely to be educational and contain informative content on healthy eating (score 3.7), compared with the other app types, although they also scored significantly lower in engagement (score 2.3). Most apps (105/124, 84.7%) displayed at least one BCT, with the most common BCT being information about health consequences.

Conclusions
Findings suggest nutrition-themed apps intended for children displayed food and beverage content not recommended by dietary guidelines, with gaming apps more likely to display not recommended foods than their nongame counterparts. Many apps have a moderate app quality, and the use of consequences (instead of rewards) was the most common BCT.
eHealth in Norway Before and After the COVID-19 Pandemic
Authors: Schopf Thomas 
Published: February 2022

Background
Regular teledermatology services were implemented in Norway in the early 1990s. Based on the available technology at the time, live interactive video consultation systems were implemented to facilitate remote consultations between dermatologists and general practitioners. With the introduction of digital cameras some years later, store-and-forward systems were introduced, but the live video systems remained popular. In the 2000s and early 2010s, there were few changes in the volume of Norwegian teledermatology services. During the 2010s, private teledermatology companies emerged, which provided both store-and-forward and live interactive video consultations. While previous services involved specialists and general practitioners, the new services now offered to patients enable them to interact with dermatologists directly.

Objective
This lecture aimed to provide a brief overview of the development of telemedicine in Norway before and during the COVID-19 pandemic with special focus on teledermatology.

Methods
This lecture provides a brief history of telemedicine in Norway with special attention to the impact of the ongoing COVID-19 pandemic. The content is based on personal experiences and literature references.

Results
The COVID-19 pandemic has had a profound impact on all parts of society. In Norway, it has also affected the way telemedicine is practiced. When the number of new infections increased substantially in early 2020, Norway was under lockdown. This had major consequences on the health care system. In response, the Norwegian government and health authorities strongly encouraged the use of telemedicine and implemented measures to support its use. Since then, there has been a large increase in the number of live video consultations both in specialist and community health care.

Conclusions
When the necessary technical infrastructure is in place, the remaining barriers to telemedicine use, such as reimbursement and integration of health care systems, can easily be overcome, which would result in high adoption rates of telemedicine.

Conflicts of Interest
TS is a partner of the Norwegian teledermatology provider “Askin.”
Unbuilt Environments and the Place of Settler Colonial Communications: A Case Study of Nineteenth-Century Medicine Hat
Authors: Griffith Jane 
Published: February 2022

Background: Though initial construction began on a colonial boarding school for Indigenous students, known as an Indian boarding school, in Medicine Hat in 1890, the school never ended up opening due to underfunding by the federal government.Analysis: Informed by Indigenous studies scholarship on place and media, this article uncovers archival traces of this unbuilt environment to reveal how white settlers used newspapers as well as visual media to will the school into being, particularly with techniques of re-placement on stolen Indigenous land.Conclusion and implications: This article connects the history of the school that never opened with the present day, insisting on the ongoingness of settler colonial tactics in media.
O tempo e o espaço do “eu” menino: a infância como território de criação na obra de Lobo Antunes
Authors: Brito Campos Maria Elvira 
Published: February 2022

O presente ensaio trata de questões inerentes à construção do tempo e da memória nas crônicas do escritor português contemporâneo António Lobo Antunes. É sabido que, epistemologicamente, esse gênero literário é constituído a partir da gênese do seu nome (crônica – chronos/kairós) e, desse viés temático, este trabalho busca mostrar como a memória e o tempo permeiam o vivido e o imaginado, como o encontro imagético entre avô e neto, num futuro construído por memórias.
Enhancing coding skills with CloudStor SWAN
Authors: Morgan Steven 
Published: February 2022

CloudStor SWAN (AARNet, 2022) is a research-focused web service for running analyses that is available to staff and students at many research institutes and Universities across Australia and New Zealand. In 2021, we used SWAN as a teaching tool in the master-level subject, Computational Genomics (COMP90016) at The University of Melbourne. This subject aims to teach students how to analyse large genomic datasets using best practices software tools, pipelines and student-written, custom code.

Although CloudStor SWAN was not conceived as a teaching tool, we worked with their technical staff to tailor the service to our use case. This innovative use of existing research infrastructure allowed us to effectively transition the subject to remote learning. Students and staff could log in to the service using their existing University credentials, from anywhere in the world, without the use of a VPN. The ability to access the platform from a web browser allowed for a consistent computing environment for all students regardless of operating system, and without having to worry about software installations on local machines. This presented a significantly improved experience from the custom servers that had been used in the past.

We used SWAN for weekly workshops during semester and for assessment in the form of assignments and an exam. It allowed us to format subject material in Jupyter notebooks where we could seamlessly integrate text, graphics and code. Additionally, assessed code questions can incorporate automatic marking and written submissions can be checked for plagiarism. SWAN also allowed us to introduce students to the UNIX command line, an important skillset that was not previously taught in the University of Melbourne Master of Science (Bioinformatics) program.

From a student perspective, SWAN allowed for a practical skillset to be developed alongside theoretical knowledge from other aspects of the course. The platform was simple to learn and allowed students to focus on the subject content and the tasks asked of them, rather than on the interface. From a teacher’s perspective, having a unified platform allowed for a single set of clear instructions, improved troubleshooting and clearer management of tool versions and software dependencies. The use of Jupyter notebooks simplified lesson plans and assessments by integrating multiple elements into single documents. This element also made the lessons more easily sharable between colleagues and collaborators.

Our integration of this technology into our tertiary teaching has served as a model for a similar use at a different Australian university. We hope to share the lessons learned from this subject, the advantages of using CloudStor SWAN in a teaching environment for both staff and students and provide some advice for others who may want to adapt it to fit their own teaching needs.
Presentation link: https://youtu.be/8tutCO1hd9c
References

AARNet. (2022). CloudStor: Access, store, share and work with your data in one place. https://www.aarnet.edu.au/network-and-services/cloud-services/cloudstor
“No sé a dónde se fue el tiempo”: testimonios de estudiantes durante la cuarentena
Authors: Cane Julieta 
Published: February 2022

El presente ensayo explora las formas de concebir el tiempo desde diferentes condiciones sociohistóricas. En particular se centra en el contexto de la pandemia por COVID-19 a partir de la lectura de los testimonios de las y los estudiantes de comunicación de universidades argentinas sobre las formas de experimentar el tiempo durante la cuarentena. Este análisis es producto de la colaboración de la Facultad de Periodismo y Comunicación Social (UNLP) en la investigación interuniversitaria “Cómo se informan y cómo estudian los estudiantes de periodismo de las universidades argentinas a través de dispositivos digitales” (Investigar en Red)[1].

[1] www.investigarenred.ar
Prestigio y tecnología
Authors: Oliveros Fortiche Diana Esperanza 
Published: February 2022

Este artículo resultado de una investigación doctoral se apoyó en la teoría del consumo estatutario para describir el impacto social de la apropiación de los dispositivos móviles en las prácticas de prestigio y diferenciación de grupos pertenecientes a clases sociales. Respondió al uso de una metodología de corte mixto, valiéndose de técnicas cualitativas de entrevista biográfica a seis personas y cuantitativas y de encuesta realizada a 122 personas. Entre sus principales resultados se evidenció como las prácticas de emulación alrededor del uso, compra y gasto excesivo en modelos y marcas, así como la necesidad cotidiana de ostentación de estos dispositivos contribuyen a reproducir y naturalizar los órdenes de desigualdad social en contextos de profunda inequidad como la afrontada en Colombia
Álbumes de vida como portales de memorias
Authors: Bracaccini Acevedo Maria 
Published: February 2022

En el presente trabajo presento algunas de las preguntas y reflexiones que se desprenden de mi actual plan de doctorado en Antropología en la Universidad Nacional de Córdoba (Arg.). En dicho trabajo busco indagar sobre la construcción y transmisión de memorias en el Sitio Memoria ex D2 ubicado en la ciudad de Córdoba; analizo específicamente las formas de recordar y representar a las personas desaparecidas. En cuanto a la metodología, propongo un acercamiento etnográfico a los significados que evocan uno de los dispositivos museográficos allí presentes para tal fin: los Álbumes de Vida. A través de estos Álbumes, que se exponen en una de las salas del Sitio, podemos acceder a las historias de vida de personas que desaparecieron en la última dictadura militar del país (1976-1983).
A lo largo del artículo ofrezco una reflexión general sobre esos Álbumes de Vida. Mi interés radica en analizar los sentidos que estas materialidades compuestas por historias y objetos de la vida cotidiana, evocan desde el lugar que hoy asumen en un espacio estatal a la consulta de las y los visitantes.
Sostenibilidad social y prácticas periodísticas
Authors: Silva Cantoni Mariana Inés 
Published: February 2022

Este artículo propone dar cuenta de la relación existente entre las miradas sobre la sostenibilidad de medios comunitarios, asociadas a paradigmas sociocríticos y la sociología de la producción noticiosa, vinculada originalmente a los estudios funcionalistas de la comunicación. Partimos de la hipótesis de que es posible revisar y problematizar los estudios de rutinas desde miradas alusivas al ejercicio del periodismo de contrainformación.
Desde un enfoque sociocrítico el texto tiene por objetivo describir de qué manera la inserción social y territorial de radio Comunitaria La Lechuza de San Juan, Argentina, sus formas de articulación con la comunidad, con las organizaciones, con otras emisoras o redes del sector y con el Estado influyen sobre las prácticas periodísticas que desarrollan quienes integran la emisora.
La investigación, de carácter cualitativo y de alcance descriptivo-relacional, aborda el objeto desde un enfoque etnográfico, empleando diversas técnicas de registro de la información.
Gran parte del trabajo de campo lo desarrollamos durante el gobierno de la Alianza Cambiemos que representó un importante retroceso en materia de derechos humanos y comunicacionales, en particular, que afectó de manera significativa a los medios comunitarios del país.
Application of Lane-Emden differential equation numerical method in fair value analysis of financial accounting
Authors: Xu Linying , Aouad Marwan 
Published: February 2022

Abstract
In order to study the fair value analysis of financial accounting, the Euler wavelet method is proposed to solve the numerical solutions of a class of Lane-Emden type differential equations with Dirichlet, Neumann and Neumann-Robin boundary conditions. The results show that the fractional integral formula of Euler wavelet function under the Riemann-Liouville fractional order definition and the L∞ and L2 errors of Haar wavelet are derived by the analytic form of Euler polynomial. By fixing M=4 and increasing the resolution scale k of Euler wavelet, a stable convergence solution can be obtained. The Lane-Emden equation with boundary conditions is transformed into algebraic equations by Euler wavelet collocation method, and the numerical results are compared with the results and exact solutions of other methods. The application advantages of fair value can be exerted through financial accounting to promote the transformation and upgrading of enterprises and realise the stable economic growth.