bluuebunny/crossref_metadata_2025_split huggingface dataset:
Invitro regeneration and standardization of Agrobacterium mediated transformation of immature embryo callus from Sweet sorghum
Authors: Yadala Ramakrishna , Bandi Jyotsna , Palle Surender Reddy , Syed Asha , Naravula Jalaja 
Published: December 2022

Abstract
Sweet sorghum is the present target and cheap source for biofuel production. Tissue culture recalcitrance and low levels of transformation reproducibility of protocols are main constrains for transgenic sweet sorghum development. Production of a transient transgenic sweet sorghum for the development of disease resistant transgenics was the aim of our studies. In this study we have developed an efficient regeneration and transformation system for in vitro culture of sweet sorghum from the immature embryo callus. Histological studies of sweet sorghum embryogenic calli revealed that the development of embryogenic shoots. Murashige and Skoog nutrient agar medium with different concentrations of 2mg/l 2,4-D and combination of 0.2 mg/l NAA and 2 mg/l Kinetin gave higher frequency of callus induction and shoot regeneration. Embryogenic callus was competent to accept the DNA with GV2600 strain. These tissues are susceptible to Agrobacterium mediated transformation carrying pCAMBIA1301 with gus gene construct as well as for shoot multiplication. For effective transformation, GV2600 strain carrying pCAMBIA1301 at 0.6 O.D. was found to be compatible in giving sweet sorghum transgenics. Molecular confirmation was done by Polymerase Chain Reaction and Southern blotting for the putative plants. The regenerated plantlets survived during acclimatization were growing similar to the normal plants. All the major parameters that affecting transformation frequency were optimized for the development of perfect sweet sorghum transgenics.
State Revenue Dependency on Resource: New Panel Evidence for Government Role in Resource – Inequality Nexus
Authors: Nguyen Khanh Hoang Dien 
Published: December 2022

Abstract
Despite the intensive amount of research on the resource-inequality nexus and the role of governance quality in modulating this linkage, their findings have been mixed at best. To further investigate the institution’s influence in this resource-inequality connection, this paper uses panel evidence from data on 77 countries over the period of 1996–2020, with novel dependent and independent variables which are directly tied to the government: resource revenue as a share in government income instead of in GDP as the independent variable, and absolute and relative redistribution as dependent variables in addition to the conventional GINI coefficients. Results from fixed-effects models on the panel dataset, both without and with lags, indicate a negative linear relationship between resource revenue and income inequality, but also suggest a threshold at which bad governance would overturn this beneficial effect. Analysis of potential mechanisms and policy recommendations are also discussed.
RNA-sequencing comparison of pneumonectomy and bleomycin reveals anti-fibrotic mechanisms of lung fibroblasts
Authors: Wellmerling Jack H , Dresler Sara R , Meridew Jeffrey A , Choi Kyoung M , Haak Andrew J , Tschumperlin Daniel J , Tan Qi 
Published: December 2022

Abstract
Background
Idiopathic pulmonary fibrosis (IPF) is characterized by pathological accumulation of scar tissue in the lung parenchyma. Many of the processes that are implicated in fibrosis, such as matrix deposition also occur following pneumonectomy (PNX). However, fibrosis does not occur. Since fibroblasts are the major cell type responsible for extracellular matrix production, we hypothesized that comparing their responses to PNX and bleomycin (BLM) would unveil key differences in the role they play during regulated and fibrotic lung growth.
Methods
RNA-sequencing was performed on flow cytometry-sorted fibroblasts from mouse lungs 14 days after bleomycin challenge, PNX, or sham. Pathway and transcription factor binding motif enrichment analysis were performed to characterize RNA-sequencing data. Normal human lung fibroblasts (NHLFs) were used as a cell culture model to validate targets.
Results
RNA-sequencing analysis revealed similar biological processes to be involved in both responses, including signaling by transforming growth factor-β (TGF-β1) and tumor necrosis factor-α. Transcription factor binding motif enrichment predicted erythroid transformation specific (ETS) superfamily members to play a key role in the response to BLM, whereas nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) were predicted to orchestrate much of the response to PNX. Itpkc, encoding inositol triphosphate kinase C, was a gene uniquely up-regulated by PNX and a likely AP-1 target. ITPKC overexpression in NHLFs antagonized the fibrotic effect of TGF-β1. RNA-sequencing analysis of primary NHLFs overexpressing ITPKC further supported its role in repressing collagen production and predicted it as an activator of canonical NF-κB signaling. ITPKC overexpression showed considerable overlap with the innate immune signaling seen following PNX.
Conclusion
Taken together, our RNA-sequencing analysis suggests that during post-PNX lung growth, AP-1 activates ITPKC to promote canonical NF-κB signaling to prevent fibrogenesis. Future studies to understand this lack of ITPKC induction during fibrotic injury may identify attractive therapeutic targets.
WITHDRAWN: Growth, Structural, Spectral, Quantum Chemical Analysis, first and second order hyperpolarizabilities and molecular Docking Study of Ammonium P- Toluenesulfonate
Authors: BOUKABCHA Nourdine , GANDHI Saravana Kumar , BELHACHEMI Mohammed hadj Mortada , MURUGAN Jeyalaxmi , AZAYEZ Mansour 
Published: December 2022

Abstract
In this work, Ammonium p-Toluenesulfonate (AMPTS) were studied theoretically and compared to experimental data. The density functional theory (DFT) method was employed to study the optimized structure using the basis set B3LYP/6–311G (d,p). DFT studies have shown strong concordance between Fourier Transform Raman, Infrared and UV-visible spectroscopic data and experimental results. The calculated energy gap of HOMO-LUMO, DOS and MEP surface, has showed that the charge transfer take place in the AMPTS molecule. The calculated global chemical reactivity descriptors have revealed that our title molecule possesses an elevated electrophilic character, as proved by its value (2.713 eV). The rise in temperature leads to an increase in thermodynamic parameters. Moreover, analysis of first and second order polarization has shown considerable potential for use in NLO applications. Hirshfeld surface has revealed the percentage contribution of the most dominant interactions as H⋯O/O⋯H, H⋯H and C⋯H/H⋯C. Finally, Molecular docking of AMPTS ligand with Feruloyl Esterase for the treatment of plant diseases.
FMR in thin films with finite extension
Authors: Fuentes G. P. , Silva D. B. O. , Padrón-Hernández E. 
Published: December 2022

Abstract

Ferromagnetic resonance (FMR) by micromagnetic simulation was carried out in Py circular-shaped films with 2 µm area and thickness of 50 nm. Another geometry with stadium-like format (1 µm of minor axes and 4 µm of major axis) was studied. Ferromagnetic resonance by simulation with an in plane and perpendicular external magnetic field show differences in results if compared the two geometries. The results are relevant, as the dimensions of these objects present an aspect ratio of more than 20 times (side dimension/thickness). The results are quite different as the magnetization maps are different for the two geometries. Even working in the field enough to saturate the material. Analyzes were performed using Kittel's equations for FMR.
Evaluation of oxidant and antioxidant status in naturally infected Indian water buffalo (Bubalus bubalis) with liver cystic echinococcosis
Authors: Aslam Humera , Bi Sabiha , Irshadullah Malik 
Published: December 2022

Abstract

The present study aims to evaluate the changes in the antioxidants and oxidant status of buffalo, naturally infected with cystic echinococcosis. Infected and non-infected livers were used to determine the level of oxidative stress markers, antioxidant and pathological marker enzymes. A significantly higher level of glutathione-s-transferase (GST) and glutathione peroxidase (GPx), while a significantly low level of glutathione reductase (GR), thioredoxin reductase (TR) was observed in the parasitized than the healthy liver. Reduced glutathione (GSH), a key non-enzymatic antioxidant, was found less in the infected than in the non-infected liver. The hydatid cysts infection is accompanied by the enhanced production of ROS with subsequent elevation of lipid peroxidation and protein oxidation, as evident from increased malondialdehyde (MDA) and protein carbonyl (PC) respectively. Enhanced MDA disrupts the cell membrane leading to the release of marker enzymes (AST, ALT, ACP, and ALP) of liver damage due to mechanical pressure and the space-occupying affect of hydatid cysts. Alteration in the level of antioxidants and oxidative stress markers provide reliable evidence for the circulating oxidative stress in the infected buffalo
Tropheryma Whipple dystrophy and Nocardia infection associated with lung tumor: a case report and literature review
Authors: Zhou Yuli , Yang Nan , Ruan Senlin , Wu Shenghai , Yu Daojun , Jin Juan 
Published: December 2022

Abstract
Tropheryma whipplei (TW) and Nocardia are rare pathogenic bacteria found in the clinic. Under the action of some immune mechanisms, TW-induced Whipple dystrophy may develop to the Whipple disease. Increasing studies have demonstrated their etiological characteristics and the epidemiology, pathogenesis, diagnosis, and treatment of the diseases they cause. We present a case of a patient with a lung tumor associated with TW and Nocardia co-infections. The final diagnosis was confirmed by tissue biopsy, PCR, and gene sequencing. Clinical features of this case are discussed, including TW diagnosis process, morphological characteristics of infection, and its relationship with tumor, as well as a review of previous studies on the Whipple disease.
Numerical Experiments on Ion-Beam Features Emitted from Different-Energy DPF Devices for Deuterium Gas
Authors: Altarabulsi A. , Abou-Ali Y. , Salo S. Alsheikh , Akel M. , Lee S. , Marciniak L. 
Published: December 2022

Abstract
In this study, numerical experiments on ion beam features emitted from nine Dense Plasma Focus devices were carried out using the Lee model code (version RADPFV5.16FIB). A simulation (numerical experiment) and connected fitting procedure of a total discharge current waveform was presented on the example of the PF-24 device, and summary data concerning simulations and fitting procedures for each device were presented. The full details of the ion beam properties as a function of pressure for the PF24 device were presented. The properties of deuterons such as flux, fluence, flux energy, fluence energy, current density, ion current, damage factor, and energy of deuterons versus pressure were computed and investigated. A comparison between the properties of deuterons computed at fitted pressure and at a pressure where the flux is the highest was presented and discussed according to equations on which the Lee model is based.
Juvenile ossifying fibroma in adult patient: a rare case report
Authors: Sh Milad Etemadi , Teimoori Farshad , Razavi Seyed Mohammad , Tajmiri Golnaz , Noghabaee Alireza , Sehat Mohammad Soroush 
Published: December 2022

Abstract
Juvenile ossifying fibroma of the jaws is an aggressive variant of ossifying fibroma that tends to affect children and adolescents. Early detection and immediate treatment are essential for this condition due to its different clinical behavior, highly aggressive nature, and high tendency for recurrence. Our report describes a case of juvenile ossifying fibroma in a 27-year-old woman who presented with swelling on the left side of the face, with typical clinical, radiological, and histological features. The patient underwent surgery to remove the lesion, and after resection of the involved area, no further symptoms were reported in the follow-up sessions. CBCT scans and panoramic imaging of the patient 6 months after surgery revealed no radiographic sign of recurrence. Autologous iliac crest grafting was performed for reconstruction of the resected area.
Machine Learning vs. Rule-Based Methods for Document Classification of Electronic Health Records within Psychiatry - A Systematic Literature Review
Authors: Rijcken Emil , Zervanou Kalliopi , Mosteiro Pablo , Scheepers Floortje , Spruit Marco , Kaymak Uzay 
Published: December 2022

Abstract

Throughout the history of artificial intelligence, various algorithm branches have predominantly been used at different times. The last decade has been characterized by a shift from rule-based methods to self-learning methods. However, while the shift towards using ML methods is evident, there is no comparison of both methods for document classification. This systematic literature review focuses on the document classification in healthcare notes from electronic health records within psychiatry. We assess how these methods compare to each other in terms of classification performance and how they have developed throughout time, and we discuss potential directions of the field. We find that rule-based methods have had a higher performance for most of the last decade than machine-learning methods.Yet, the shift in representation techniques and algorithms used in recent years resulted in machine learning methods performing better.Dense document representation techniques, with mostly non-zero cells, outperform sparse representation techniques, with mostly zeros. Also, many neural networks outperform other self-learning- and rule-based methods. We find that state-of-the-art language models are barely employed in the psychiatric domain and expect an increase in the application of federated learning can increase the data availability for model training.
Rapid Point of Care Testing for Sexually Transmitted Infections Using a Portable Isothermal Loop Mediated Nucleic Acid Amplification Platform
Authors: Germaner Ece T. Esitgen , Wassill Lars , Dichtl Karl , Roider Julia , Seybold Ulrich 
Published: December 2022

Abstract
Purpose
To analyze sensitivity and specificity of the rapid point-of-care (POC) eazyplex STD complete testing platform for sexually transmitted infections (STI) among men who have sex with men (MSM).
Methods
272 anal, urethral, and pharyngeal swabs collected from 153 MSM were tested by both eayzplex STD complete and the university microbiology laboratory.
Results
Compared to the reference diagnostic method, the overall sensitivity/specificity of STD complete was 88%/98% for N. gonorrhoeae, 82%/100% for C. trachomatis, 70%/>99% for U. urealyticum, and 85%/98% for M. hominis, respectively. Sensitivity for N. gonorrhoeae and U. urealyticum in urethral samples was 100%.
Conclusion
With good to excellent sensitivity depending on sampling site and pathogen as well as excellent specificity overall eazyplex STD complete is a useful rapid diagnostic method for POC STI-testing especially for N. gonorrhoeae and C. trachomatis, allowing for almost immediate treatment initiation.
Effect of Pomegranate (Molasses, White Peel and Red Peel) extract on healthy liver and hepatotoxicity induced by Phenylhydrazine in male rats
Authors: Soliman Nabil Abbas , Mansour Sherif Wajih , Ammar Mohammed Ahmed , Ammar Mohamed Ahmed Mohamed 
Published: December 2022

Abstract
Pomegranates (Punica granatum L.) are said to provide various health advantages, according to accumulating research. The Liver tissue and antioxidant stress effect of pomegranate molasses, white and red peel extracts on male rats with phenylhydrazine (PHZ)-induced anaemia are highlighted in this article. Phenylhydrazine induced anemia resulted in: Decreasing of Direct Bilirubin, CAT, GPx, SOD, ALP, Accompanied by increasing of LDH, Total Bilirubin, Indirect Bilirubin, MDA, ALT, AST. Regarding the histopathology study of Liver: PHZ exposed group showed a loss of the normal architecture with dilated, congested central vein and necrotic areas, dilated and congested blood sinusoids with pycnotic Kuppfer cells and hepatocytes with karyolytic nuclei. PHZ exposed group showed a severely-vacuolated hepatic environment with hydrobic degeneration apoptotic and degenerated hepatocytes, focal necrosis, dilated blood sinusoids with atrophied Kuppfer cells and dilated central vein. Regading of Molasses Pomegranate + PHZ; White Peel Pomegranate + PHZ and Red Peel Pomegranate + PHZ (Improvement Group): There was an increase of Direct Bilirubin, CAT, GPx, SOD, ALP. Accompanied by decreasing of LDH, Total Bilirubin, Indirect Bilirubin, MDA, ALT, AST, ALP. Red peel pomegranate after PHZ (Red Peel + PHZ) exposed group showed a partial restoration of the normal histological pattern to a great extent with a central vein having a good profile and hepatocytes with healthy and intact nuclei. But some vacuolization and leucocytic infiltration is still evident. White peel pomegranate after PHZ (White Peel + PHZ) exposed group showed a regeneration in hepatocytes, a nearly normal blood sinusoids, but the central vein is still congested and surrounded with damaged cells. Molasses pomegranate after PHZ (Molasses + PHZ) exposed group showed most of hepatocytes and Kuppfer cells taking normal appearance but the hepatic environment is still vacuolated (V) and the central vein is congested. Conclusion: Molasses, White peel and Red peel Pomegranate have a strong antioxidant for protective liver tissue.
The effect of pelvic floor muscle training in women with functional bladder outlet obstruction
Authors: Tzelves Lazaros , Tsikopoulos Ioannis , Sakalis Vasileios , Papathanasiou Christina , Samarinas Michael 
Published: December 2022

Abstract
Introduction and Hypothesis
Female voiding dysfunction is often due to bladder outlet obstruction (BOO). We investigated pelvic floor muscle training (PFMT) effectiveness in women with functional BOO.
Methods
This is a prospective study recruiting 63 women functionally obstructed, over 18yo, maximum flow rate (Qmax) less than 12ml/sec, naïve of voiding treatment. Exclusion criteria were anatomical BOO, neurological condition, pelvic intervention, psychiatric or anticholinergic medication, diabetes mellitus and affected upper urinary tract. At baseline, women underwent uroflow, post void residual (PVR) measurement, cystoscopy, cystogram and urodynamic study (UDS) with pelvic electromyography (EMG). Blaivas-Groutz nomogram has been used to define obstruction. After diagnosis, patients underwent six-month PFMT. Re-evaluation was offered four weeks after end of treatment. Data were analyzed with SPSSv22.0.
Results
63 women were recruited and 48 finally included. At baseline, 20 reported 3 urinary tract infections (UTIs) during last year, and 12 had one episode of urine retention. Median Qmax was 7.5ml/sec and median PVR 110ml. 40 women were obstructed. 16 (40%) had mild, 16 (40%) moderate and 8 (20%) severe obstruction. All subjects had an overactive pelvic floor on EMG.
Obstructed women were re-evaluated. Median Qmax was 8.5ml/sec, close to baseline (p= 0.16). Median PVR was 65ml, reduced to baseline (p= 0.02). 33 (82.5%) remained obstructed, 22 (66.67%) with mild, 8 (24.24%) moderate and 3 (9.09%) severe obstruction. 7 (17.5%) were non-obstructed. 4 patients reported one UTI episode with no cases of retention.
Conclusions
A six-month PFMT reduced UTIs and PVR in women with functional BOO. Additionally, most patients had a de-escalation to milder obstruction.
Health Providers’ Perspectives on Quality of Adolescent Mental Health Services in Low- Resource Settings: a qualitative study in São Paulo City, Brazil
Authors: Quinlan-Davidson Meaghen , Devakumar Delan , Mannell Jenevieve , Peres Maria Fernanda , Kiss Ligia 
Published: December 2022

Abstract
Background: Quality in adolescent mental health services is instrumental to adolescents accessing and continuing care. There has been a growing interest and investment in adolescent mental health services, yet evidence on their quality remains inadequate, particularly in limited resource settings such as São Paulo city, Brazil. Health services in these settings experience a lack of financial and human resources. Adolescents within these settings are often exposed to poverty, community violence and limited access to resources and essential services; all of which contribute to the development of mental health conditions. The objective of the study was to explore health care providers definition of quality, as well as barriers and enablers to adolescent mental health services within resource-limited settings of São Paulo city, Brazil.
Methods: Health care providers were purposively sampled in five primary health care facilities and two specialised mental health facilities, predominantly in the southwest zone of São Paulo city. Semi-structured interviews among 45 health care providers were conducted. Thematic analysis was used to explore their narratives.
Results: No standardised definition of quality in adolescent mental health services emerged in the interviews. Definitions of quality revolved around a social determinants of mental health perspective; and aspects of service organisation including a trained, multidisciplinary workforce and person-centred care. These definitions were ideal and impossible to realise due to barriers identified by the health care providers, including underfunded and overburdened systems of care; the prioritisation of other age groups as set out by the primary care mandate; and the social and environmental context which adversely affected the provision of care. Ultimately, this resulted in the neglect of the adolescent by the health system.
Conclusions: This is the first study to investigate health care providers’ definition of quality in adolescent mental health services in Brazil and in low-resource and violent settings. Based on health care providers’ definitions, a social determinants of health approach, in combination with a trained workforce and person-centred care, is key to addressing quality in adolescent mental health services. The findings have implications for local conceptualisations of quality, barriers to adolescent mental health services and reducing the treatment gap.
Liner shipping hub location and empty container repositioning: Use of foldable containers and container leasing
Authors: Wang Qian , Zheng Jianfeng , Lu Bo 
Published: December 2022

Abstract
Considering the influence of economies of scale and ship capacity on empty container repositioning, using foldable containers and container leasing may be effective ways to save the operating costs of liner shipping companies. Considering the minimization of the transportation costs of empty and laden containers, this paper studies a liner shipping hub location problem with empty container repositioning (LSHLPECR) considering foldable containers. We propose a mixed integer linear program (MILP) for the formulation of the LSHLPECR. In terms of the optimal solution of our MILP, this paper further explores the container leasing pricing problem (CLPP), where our aim is to obtain perceived container leasing prices (PCLPs) utilizing the inverse optimization technique. Numerical experiments verify the effectiveness of the studied problems.