bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation, Identification and Quantitative Determination of Anthracene Derivatives by HPLC-UV Method in the Raw Materials of Some Representatives of the Genus <i>Rumex</i> of Three Vegetation Times
Authors: Poluyanov A. M. , Sokolova A. Yu. , Malashenko E. A. , Sergunova E. V. , Bobkova N. V. 
Published: November 2022

Introduction. The creation of new highly effective drugs requires a thorough study of the metabolome of plant raw materials and a comparative phytochemical study of the underground organs of closely related species of Rumex, such as: R. crispus, R. obtusifolius and R. aquaticus, ubiquitous in Russia. It was noted that they have a metabolome like the official R. confertus, which in turn confirms the potential for studying these species. Of scientific and practical interest is the study of the dynamics of accumulation of the leading group of biologically active substances – anthracene derivatives, depending on the phenological phases of plant development.Aim. Identify and quantify anthracene derivatives in the underground organs of R. confertus, R. crispus, R. obtusifolius and R. aquaticus harvested in three different phases of vegetation.Materials and methods. Extracts from the underground organs of the studied plants obtained according to the method from the pharmacopoeial article on R. confertus were used as the analyzed solutions. The solutions were analyzed on a Nexera-i LC-2040 chromatograph (Shimadzu Corporation, Japan) equipped with a column and sample thermostat, a degasser, and an autosampler using an individually selected mobile phase elution gradient (0.1 % phosphoric acid/acetonitrile solution). Primary data were processed using LabSolutions Single LC software (Shimadzu Corporation, Japan). Compounds from the group of anthracene derivatives were identified by retention times. Detection was carried out using a UV detector with a dynamic change in the absorption wavelength during analysis from 365 ± 2 nm to 254 ± 2 nm.Results and discussion. Alcohol-water extracts were obtained from the underground organs of Rumex. An elution gradient was selected for the simultaneous determination of 5 anthracene derivatives with a single analysis time of 40 minutes. These chromatographic conditions made it possible to identify and quantify the content of emodin, 8-O-β-D-glucoside of emodin, and chrysophanol in the underground organs of R. confertus, R. crispus, R. obtusifolius and R. aquaticus in three different vegetations. Glycosides of anthracene derivatives: glucofrangulin A and frangulin A were not found in the studied objects.Conclusion. Anthracene derivatives were isolated from the underground organs of different vegetations, a method for the quantitative determination of anthracene derivatives in alcohol-water extracts was developed, emodin, 8-O-β-D-glucoside of emodin and chrysophanol were found and quantified.
An Investigation of the Relationship Between Gender Roles Attitudes and Self-Esteem of Married
Authors: ERCAN Merve , KOÇAK Orhan , ERCAN Fatümatü Zehra 
Published: November 2022

This study aimed to investigate the relationship between the gender roles attitudes (GRA) and self-esteem of married women. The study has a quantitative design. GRAS, Self-Esteem Scale, and the Demographic Questionnaire were used to collect data. Correlation, regression, and ANOVA analyses were performed to analyze the data. The correlation analysis performed to understand the relationship between GRA and self-esteem has revealed a positive and weak relationship between the sub-dimensions of GRA and income and education status; while a weak negative relationship has found between the sub-dimensions of GRA and years of marriage and the number of children. The hierarchical regression analysis carried out to understand the predictive power of GRAS sub-dimensions and demographic characteristics on self-esteem showed that two dimensions of GRAS, namely TGR and egalitarian gender roles, significantly and moderately predicted self-esteem of married women in marriage, while the variables of years of marriage, number of children, education status and income were ound to predict self-esteem significantly at a low level.
Compact automated culture machine for human induced pluripotent stem cell maintenance and differentiation
Authors: Bando Kazunori , Yamashita Hiromi , Tsumori Motomu , Minoura Hayase , Okumura Koji , Hattori Fumiyuki 
Published: November 2022

The technologies used to generate human induced pluripotent stem cell (iPSC) from somatic cells potentially enable the wide application of iPSC-derived differentiated cells in industrial research fields as a replacement for animals. However, as highly trained individuals are required to obtain reproducible results, this approach has limited social implementation. In the research field of iPSC, it is believed that documentable information is not enough for reproducing the quality of the differentiated cells. Therefore, automated culture machines for cell processing should make the starting of iPSC-using researches easier. We developed a programmable all-in-one automated culture machine, with dense and compact constitution that fits within a normal biosafety cabinet (200 mm wide, 233 mm height, and 110 mm depth). This instrument was fabricated using novel x-y-z-axes-rail-system, such as an overhead traveling crane, in a factory, which served as the main handling machinery. This machine enabled stable and efficient expansion of human iPSC under the feeder-free condition, without karyotype alterations, and simultaneously differentiated the cells into various cell types, including cardiomyocytes, hepatocytes, neural progenitors, and keratinocytes. Overall, this machine would facilitate the social implementation of human pluripotent stem cells and contribute to the accumulation of sharable knowledge for the standardization of the entire handling processes of iPSC in pharmaceutical, food, and cosmetic research.
Early administration of ivabradine in patients admitted for acute decompensated heart failure
Authors: Yang Teng-Yao , Tsai Meng-shu , Jan Jeng-Yu , Chang Jung-Jung , Chung Chang-Ming , Lin Ming-Shyan , Chen Hui-Ming , Lin Yu-Sheng 
Published: November 2022

BackgroundHeart rate (HR) control is important in heart failure (HF) patients with reduced ejection fraction, and ivabradine is indicated for patients with chronic HF and sinus rhythm. However, ivabradine is limited in initiation of ivabradine at acute stage of HF.Materials and methodsThis multi-institutional retrospective study enrolled 30,639 patients who were admitted for HF from January 01, 2013 to December 31, 2018 at Chang Gung Memorial Hospitals. After applying selection criteria, the eligible patients were divided into ivabradine and non-ivabradine groups according to the initiation of ivabradine at the index hospitalization. HR, clinical outcomes including HF hospitalization, all-cause hospitalization, mortality, the composite of cardiovascular (CV) death or HF hospitalization and newly developed atrial fibrillation, and left ventricular ejection fraction (LVEF) and left atrium size were compared between the ivabradine and non-ivabradine groups after inverse probability of treatment weighting (IPTW) analysis after 12 months.ResultsThe HR at admission in the ivabradine group (n = 433) was 99.04 ± 20.69/min, compared to 86.99 ± 20.34/min in the non-ivabradine group (n = 9,601). After IPTW, HR was lower in the ivabradine group than that in the non-ivabradine group after 12 months (74.14 ± 8.53 vs. 81.23 ± 16.79 bpm, p = 0.079). However, there were no significant differences in HF hospitalization (HR = 1.02; 95% CI, 0.38–2.79), all-cause hospitalization (HR = 0.95; 95% CI, 0.54–1.68), mortality (HR = 0.87; 95% CI, 0.69–1.08), the composite of CV death or HF hospitalization (HR = 0.87; 95% CI, 0.69–1.08) and newly developed AF between the two groups. In addition, LVEF increased with time in both groups, but there were no significant differences during the observation period.ConclusionIvabradine was beneficial in controlling HR when initiated in patients with acute stage of HF, but it did not seem to provide any benefits in reducing HF hospitalization, all-cause hospitalization, and mortality in 1 year after discharge.
Tibio-pedal arterial pressure assessment during endovascular intervention to improve quality-of-life in patients with intermittent claudication
Authors: Kwan Tak W. , Lee Samuel , Lin Patricia , Liou Michael , Siu Henry , Patel Apurva , Ruzsa Zoltan 
Published: November 2022

ObjectiveThe aim of this study is to compare the quality-of-life (QOL) outcomes and the tibio-pedal arterial pressure post-endovascular intervention.BackgroundPhysiological assessment of peripheral arterial lesions is infrequently performed during endovascular interventions.Materials and methodsWe retrospectively reviewed all 343 patients with intermittent claudication who underwent an endovascular intervention via tibio-pedal artery access from October 2018 to May 2021. The baseline and post-intervention tibio-pedal arterial pressures from the pedal sheaths were measured. QOL was assessed using a pre-validated Walking Impairment Questionnaire (WIQ) score before and at 30-day after intervention. We compared the baseline tibio-pedal arterial pressure, post-intervention tibio-pedal arterial pressure, delta pressure (post-intervention minus baseline), baseline WIQ scores, 30-day WIQ scores, and delta score (30-day minus baseline).ResultsAll 343 patients had successful tibio-pedal accesses. The average tibio-pedal arterial pressure at baseline was 87.0 ± 1.8 mmHg vs. 135.5 ± 1.7 mmHg post-intervention (p &lt; 0.001). Average baseline and 30-day WIQ scores were summation (99.8 ± 3.3 vs. 115.0 ± 3.1, p &lt; 0.001), walking distance (35.7 ± 1.3 vs. 42.5 ± 1.3, p &lt; 0.001), walking speed (21.1 ± 0.9 vs. 23.6 ± 0.8, p = 0.036), stair climbing (4.7 ± 1.4 vs. 24.2 ± 1.4, p = 0.019), and symptoms (18.8 ± 0.2 vs. 20.1 ± 0.2, p &lt; 0.001), respectively. When comparing the increased post-intervention tibio-pedal arterial pressure &lt;60 mmHg vs. ≥60 mmHg, the average delta WIQ scores were all significantly improved with summation (10.0 ± 3.9 to 25.8 ± 5.5, p = 0.01), walking distance (4.1 ± 1.7 to 9.8 ± 2.5, p = 0.02), walking speed (1.5 ± 1.1 to 4.3 ± 1.5, p = 0.02), stair climbing (2.3 ± 1.8 to 9.4 ± 2.5, p = 0.02), and symptoms (1.0 ± 0.3 to 1.8 ± 0.4, p = 0.04), respectively.ConclusionIncreasing the post-intervention tibio-pedal arterial pressure by 60 mmHg can enhance QOL as suggested by improvement of WIQ scores.
Augmented detection of septal defects using advanced optical coherence tomography network-processed phonocardiogram
Authors: Huang Po-Kai , Yang Ming-Chun , Wang Zi-Xuan , Huang Yu-Jung , Lin Wei-Chen , Pan Chung-Long , Guo Mei-Hui 
Published: November 2022

BackgroundCardiac auscultation is a traditional method that is most frequently used for identifying congenital heart disease (CHD). Failure to diagnose CHD may occur in patients with faint murmurs or obesity. We aimed to develop an intelligent diagnostic method of detecting heart murmurs in patients with ventricular septal defects (VSDs) and atrial septal defects (ASDs).Materials and methodsDigital recordings of heart sounds and phonocardiograms of 184 participants were obtained. All participants underwent echocardiography by pediatric cardiologists to determine the type of CHD. The phonocardiogram data were classified as normal, ASD, or VSD. Then, the phonocardiogram signal was used to extract features to construct diagnostic models for disease classification using an advanced optical coherence tomography network (AOCT-NET). Cardiologists were asked to distinguish normal heart sounds from ASD/VSD murmurs after listening to the electronic sound recordings. Comparisons of the cardiologists’ assessment and AOCT-NET performance were performed.ResultsEchocardiography results revealed 88 healthy participants, 50 with ASDs, and 46 with VSDs. The AOCT-NET had no advantage in detecting VSD compared with cardiologist assessment. However, AOCT-NET performance was better than that of cardiologists in detecting ASD (sensitivity, 76.4 vs. 27.8%, respectively; specificity, 90 vs. 98.5%, respectively).ConclusionThe proposed method has the potential to improve the ASD detection rate and could be an important screening tool for patients without symptoms.
Environmental and social determinants of thyroid cancer: A spatial analysis based on the Geographical Detector
Authors: Huo Shirui , Liu Ying , Sun Anyi , Zhang Bo 
Published: November 2022

IntroductionThyroid cancer has increased sharply in China in recent years. This change may be attributable to multiple factors. The current study aimed to explore the environmental and social determinants of thyroid cancer.MethodsIncidence data from 487 cancer registries in 2016 were collected. Eight factors were considered, namely, air pollution, green space, ambient temperature, ultraviolet radiation, altitude, economic status, healthcare, and education level. A geographical detector (measured by q statistic) was used to evaluate the independent and interactive impact of the eight factors on thyroid cancer.ResultsSocial factors, especially economic status and healthcare level (q &gt; 0.2), were most influential on thyroid cancer.Ultraviolet radiation, air pollution, and temperature had more impact on women, while green space and altitude had more influence on men. Enhanced effects were observed when two factors interacted. Spatially, economic status, healthcare, and air pollution were positively associated with thyroid cancer, while education level, green space, and altitude were negatively related to thyroid cancer.ConclusionThe socio-environmental determinants and spatial heterogeneity of thyroid cancer were observed in this study. These findings may improve our understanding of thyroid cancer epidemiology and help guide public health interventions.
Analysis of the key ligand receptor CADM1_CADM1 in the regulation of thyroid cancer based on scRNA-seq and bulk RNA-seq data
Authors: He Hui , Cong Shan , Wang Yu , Ji Qinghai , Liu Weiyan , Qu Ning 
Published: November 2022

IntroductionAdvanced papillary thyroid cancer (PTC) has a poor prognosis, 60~70% of which become radio iodine refractory (RAI-R), but the molecular markers that assess PTC progress to advanced PTC remain unclear. Meanwhile, current targeted therapies are badly effective due to drug resistance and adverse side effects. Ligand-receptor pairs (L/R pairs) play an important role in the interactions between tumor cells and other cells in the tumor microenvironment (TME). Nowadays, therapies targeting ligand-receptor pairs in the TME are advancing rapidly in the treatment of advanced cancers. However, therapies targeting L/R pairs applied to advanced PTC remains challenging because of limited knowledge about L/R pairs in PTC.MethodsWe screened the critical L/R pair: CADM1-CADM1 using 65311 single-cell RNA sequencing (scRNA-seq) samples from 7 patients in different stage of PTC and bulk RNA-seq datasets containing data from 487 tumor samples and 58 para-carcinoma samples. Moreover, the expression levels of CADM1-CADM1 was assessed by quantitative real time polymerase chain reaction (qRT-PCR) and the function was analyzed using Transwell immigration assay.ResultsWe found that CADM1_CADM1 could be regarded as a biomarker representing a good prognosis of PTC. In addition, the high expression of CADM1_CADM1 can strongly increase the sensitivity of many targeted drugs, which can alleviate drug resistance. And the results of qRT-PCR showed us that the expression of CADM1_CADM1 in PTC was down-regulated and overexpression of CADM1 could suppresses tumor cell invasion migration.ConclusionOur study identified that CADM1_CADM1 played an essential role in the progression of PTC for the first time and our findings provide a new potential prognostic and therapeutic ligand-receptor pair for advanced PTC.
Intranasal inoculation of IFN-λ resolves SARS-CoV-2 lung infection via the rapid reduction of viral burden and improvement of tissue damage
Authors: Shin Haeun , Kim Sujin , Jo Ara , Won Jina , Gil Chan Hee , Yoon So Yeon , Cha Hyunkyung , Kim Hyun Jik 
Published: November 2022

IntroductionThe innate immune responses of upper airway could further our understanding toward antiviral strategies against SARS-CoV-2. We characterize the potential of interferon (IFN)-λ as an innate immune inducer for the rapid clearance of SARS-CoV-2 in the lung and the therapeutic efficacy of intranasal inoculation of IFN-λ to resolve acute lung infection.MethodsSyrian golden hamsters were infected with SARS-CoV-2 and the dynamics of SARS-CoV-2 infection depending on IFN-λ inoculation were tested.ResultsSARS-CoV-2-infected Syrian golden hamsters exhibited a significant decrease in body weight and high viral mRNA level at 3 days post-infection (dpi). Although viral replication was reduced completely from 7 dpi, the pathologic findings remained prominent until 14 dpi in the lung of hamsters. The transcription of IFN-λ was significantly induced in response to SARS-CoV-2 infection with the increase of IFN-stimulated genes. Intranasal inoculation of IFN-λ restricted SARS-CoV-2 replication in the lungs of infected completely from 3 dpi with markedly reduction of inflammatory cytokines. The transcriptional phenotypes were altered to the direction of damage repair and tissue remodeling in the lungs of SARS-CoV-2-infected hamsters following intranasal inoculation of IFN-λ, which improved SARS-CoV-2-caused lung damage.ConclusionCollectively, our findings suggest that IFN-λ might be a potent innate immune inducer in the lung and intranasal inoculation of IFN-λ resolves SARS-CoV-2 infection with rapid viral clearance and improvement of lung damage.
Perioperative prothrombin complex concentrate and fibrinogen administration are associated with thrombotic complications after liver transplant
Authors: Dehne Sarah , Riede Carlo , Klotz Rosa , Sander Anja , Feisst Manuel , Merle Uta , Mieth Markus , Golriz Mohammad , Mehrabi Arianeb , Büchler Markus W. , Weigand Markus A. , Larmann Jan 
Published: November 2022

BackgroundUse of intraoperative prothrombin complex concentrates (PCC) and fibrinogen concentrate administration has been linked to thrombotic events. However, it is unknown if its use is associated with thrombotic events after liver transplant.Methods and analysisWe conducted a post hoc analysis of a prospectively conducted registry database study on patients who underwent liver transplant between 2004 and 2017 at Heidelberg University Hospital, Heidelberg, Germany. Univariate and multivariate analyses were used to determine the association between PCC and fibrinogen concentrate administration and thrombotic complications.ResultsData from 939 transplantations were included in the analysis. Perioperative PCC or fibrinogen administration was independently associated with the primary composite endpoint Hepatic artery thrombosis (HAT), Portal vein thrombosis (PVT), and inferior vena cava thrombosis [adjusted HR: 2.018 (1.174; 3.468), p = 0.011]. PCC or fibrinogen administration was associated with the secondary endpoints 30-day mortality (OR 4.225, p &lt; 0.001), graft failure (OR 3.093, p &lt; 0.001), intraoperative blood loss, red blood cell concentrate, fresh frozen plasma and platelet transfusion, longer hospitalization, and longer length of stay in intensive care units (ICUs) (all p &lt; 0.001). PCC or fibrinogen administration were not associated with pulmonary embolism, myocardial infarction, stroke, or deep vein thrombosis within 30 days after surgery.ConclusionA critical review of established strategies in coagulation management during liver transplantation is warranted. Perioperative caregivers should exercise caution when administering coagulation factor concentrate during liver transplant surgery. Prospective randomized controlled trials are needed to establish causality for the relationship between coagulation factors and thrombotic events in liver transplantation. Further studies should be tailored to identify patient subgroups that will likely benefit from PCC or fibrinogen administration.
Machine learning-based identification of the novel circRNAs circERBB2 and circCHST12 as potential biomarkers of intracerebral hemorrhage
Authors: Bai Congxia , Hao Xiaoyan , Zhou Lei , Sun Yingying , Song Li , Wang Fengjuan , Yang Liu , Liu Jiayun , Chen Jingzhou 
Published: November 2022

BackgroundThe roles and potential diagnostic value of circRNAs in intracerebral hemorrhage (ICH) remain elusive.MethodsThis study aims to investigate the expression profiles of circRNAs by RNA sequencing and RT–PCR in a discovery cohort and an independent validation cohort. Bioinformatics analysis was performed to identify the potential functions of circRNA host genes. Machine learning classification models were used to assess circRNAs as potential biomarkers of ICH.ResultsA total of 125 and 284 differentially expressed circRNAs (fold change &gt; 1.5 and FDR &lt; 0.05) were found between ICH patients and healthy controls in the discovery and validation cohorts, respectively. Nine circRNAs were consistently altered in ICH patients compared to healthy controls. The combination of the novel circERBB2 and circCHST12 in ICH patients and healthy controls showed an area under the curve of 0.917 (95% CI: 0.869–0.965), with a sensitivity of 87.5% and a specificity of 82%. In combination with ICH risk factors, circRNAs improved the performance in discriminating ICH patients from healthy controls. Together with hsa_circ_0005505, two novel circRNAs for differentiating between patients with ICH and healthy controls showed an AUC of 0.946 (95% CI: 0.910–0.982), with a sensitivity of 89.1% and a specificity of 86%.ConclusionWe provided a transcriptome-wide overview of aberrantly expressed circRNAs in ICH patients and identified hsa_circ_0005505 and novel circERBB2 and circCHST12 as potential biomarkers for diagnosing ICH.