bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERBEDAAN DARAH SEGERA DIPERIKSA, DILAKUKAN PENYIMPANAN PADA SUHU 20ºC-25ºC DAN 4ºC-8ºC SELAMA 6 JAM TERHADAP JUMLAH ERITROSIT
Authors: Afriansyah Ferdi , Bastian Bastian , Sari Indah , Juraijin Denny 
Published: January 2022

Kesalahan yang paling besar dalam pemeriksaan yang sering terjadi pada tahap pra analitik dengan kesalahan 50%-75%. Pemeriksaan sampel darah untuk menjaga kondisi supaya tidak rusak maka biasanya sampel disimpan pada lemari pendingin selama beberapa jam sampai hari, proses penyimpanan menyebabkan perubahan hasil uji karena sifat darah yang cepat rusak apabila dibiarkan dikondisi yang tidak ideal atau suhu ruangan adapun persyaratan udara ruangan yang baik memiliki suhu berkisar 18oC-28oC suhu ruangan dapat ditentukan oleh keadaan lingkungan tempat dimana proses pemeriksaan tersebut.Penundaan waktu dapat mempengaruhi jumlah eritrosit semakin lama penundaan maka jumlah sel-sel terhitung semakin berkurang karena sel mengalami hemolisis, biokimia, biomekanis dan reaksi imonologis yang akan merusak struktural atau morfologi dan juga kosentrasi antikoagulan yang tidak tepat juga dapat menyebabkan gangguan tonisitas pembengkakan sel dan hemolisis. Spesimen darah yang di simpan pada suhu kamarlebih baik pemeriksaannya segera dilakukan setelah pengambilan sampel dalam satu hari dan secepat mungkin setelah pengumpulan. Peneliti bertujuan untuk mengetahui perbedaan darah segera diperiksa, darah simpan suhu 20oC–25oC dan 4oC–8oC selama 6 jam terhadap jumlah eritrosit. Jenis penelitian ini adalah eksperimen yang dianalisa secara diskriptif kuantitatif. Penelitian ini dilakukan pada mahasiswi DIV Teknologi Laboratorium Medis yang berjumlah 30 responden.Hasil penelitian jumlah eritrositpada darah segera dengan nilai rata–rata 4,57, pada suhu ruang memiliki nilai rata–rata 4,65, pada suhu kulkas memiliki nilai rata–rata 4,50 danuji friedmandidapatkan nilai p= 0,172 Kesimpulan dari penelitian menunjukkan bahwa tidak ada perbedaan darah segera diperiksa, darah simpan pada suhu 20oC-25oC dan 4oC-8oC selama 6 jam terhadap eritrosit.
Pendidikan Islam Menurut Syaikh Muḥammad bin Ṣāliḥ al-Uthaimīn
Authors: Wardhana Windro Saputro Oscar 
Published: January 2022

Abstrak
Salah satu ulama Islam yang memberikan dampak besar didalam kemajuan pendidikan Islam adalah syaikh Muḥammad bin Sālih al-Uthaimīn yang merupakan salah satu ulama kerajaan Saudi Arabia yang memberikan sepanjang hidupnya untuk menyebarakan dakwah islam tidak hanya di kerajaan Arab Saudi saja akan tetapi juga mempengaruhi di berbagai penjuru dunia, diantaranya Indonesia. Syaikh Muḥammad Sālih al-Uthaimīn seorang guru dan pendidik yang berpengalaman yang telah bergelut dibidang pendidikan selama lima puluh tahun samapai beliau wafat -semoga Allah merahmatinya-. Dan karya-karyanya telah tersebar disluruh dunia hingga ke Indonesia, dan diantara karya-karyanya yang tersebar dan dipelajari di Indonesia bahkan sampai diterjemahkan ke bahasa Indonesia adalah Qaul al Mufid, Syarḥ Riyād as-Shāliḥīn, Syarḥ al-Arbaīn an-Nawawi dll. Penelitian ini terfokus pada pendapat syaikh Muḥammad bin Shālih al-Uthaimīn dalam asas-asas dan metode-metode pendidikan Islam. Penelitian ini termasuk penelitian pustaka yang termasuk dalam penelitian kualitatif dengan menggunakan angka-angka. Data primer dalam penelitian ini karya-karya syaikh al-Uthaimīn, khutbah-khutbah dan kajian-kajiannya. adapun data sekunder dari buku-buku dan dokumen yang berhubungan dengannya. Hasil dari penelitian ini adalah asas-asas pendidikan Islam menurut Syaikh adalah: pendidikan yang menyeluruh, pendidikan bertahap, pendidikan yang terus menerus, pendidikan yang terperbarukan dan sesuai dengan dalil. dan metode-metode islam yang disebutka oleh Syaikh metode suri tauladan, metode kajian, metode permisalan, metode praktek, metode tanya, metode memberi pembahasan dan jalan keluar, metode pembiasaan, metode hafalan
هذا البحث مركز على رأي الشيخ العثيمين في أسس وأساليب التربية الإسلامية. هذا البحث من البحث المكتبي وهو من ضمن البحث الكيفي بدراسة الأرقام. البيانات الأولية في هذا البحث هي مؤلفات الشيخ العثيمين وخطبه ومحاضراته. أما البيانات الثانوية تكون من خلال الكتب، والوثيقة المتعلقة به.
بعد إنهاء البحث وجدت النتيجة : أن أسس التربية الإسلامية التيى رآها الشيخ هي : التربية الشاملة، التربية المتدرجة، التربية المستمرة، التربية المحافظة والمجددة، التربية وفق دليل. وأساليب التربية التي ذكرها الشيخ العثيمين هي : أسلوب القدوة، أسلوب المحاضرة، أسلوب ضرب الأمثال، أسلوب التطبيق، أسلوب السؤال، أسلوب طرح المسألة والحل، أسلوب التعويد، وأسلوب تلقين الحفظ.
كلمات البحث : العثيمين، التربية الإسلامية، الأساس، الأسلوب
Black Women, Citizenship, and the Making of Modern Cuba
Authors: Brunson Takkara K. 
Published: January 2022

In Black Women, Citizenship, and the Making of Modern Cuba, Takkara Brunson traces how women of African descent battled exclusion on multiple fronts and played an important role in forging a modern democracy. Brunson takes a much-needed intersectional approach to the political history of the era, examining how Black women’s engagement with questions of Cuban citizenship intersected with racial prejudice, gender norms, and sexual politics, incorporating Afro-diasporic and Latin American feminist perspectives.
Brunson demonstrates that between the 1886 abolition of slavery in Cuba and the 1959 Revolution, Black women—without formal political power—navigated political movements in their efforts to create a more just society. She examines how women helped build a Black public sphere as they claimed moral respectability and sought racial integration. She reveals how Black women entered into national women’s organizations, labor unions, and political parties to bring about legal reforms. Brunson shows how women of African descent achieved individual victories as part of a collective struggle for social justice; in doing so, she highlights how racism and sexism persisted even as legal definitions of Cuban citizenship evolved.
Galapagos land iguanas as ecosystem engineers
Authors: Tapia Washington , Gibbs James P. 
Published: January 2022

BackgroundDeclines of large-bodied herbivorous reptiles are well documented, but the consequences for ecosystem function are not. Understanding how large-bodied herbivorous reptiles engineer ecosystems is relevant given the current interest in restoration of tropical islands where extinction rates are disproportionately high and reptiles are prominent as herbivores.MethodsIn this study, we measured the ecosystem-level outcomes of long-term quasi-experiment represented by two adjacent islands within the Galapagos Archipelago, one with and the other without Galapagos land iguanas (Conolophus subcristatus), large-bodied herbivores known to feed on many plant species. We characterized plant communities on each island by developing high-resolution (<1 cm2) aerial imagery and delineating extent of plant associations and counting individual plants on each.ResultsIn the presence of iguanas there was dramatically less woody plant cover, more area with seasonal grasses, and many fewer cacti. Cacti had a more clumped distribution where iguanas were absent than where iguanas were present.DiscussionThis study provided strong evidence that Galapagos land iguanas can substantially engineer the structure of terrestrial plant communities; therefore, restoration of large-bodied reptilian herbivores, such as land iguanas and giant tortoises, should be regarded as an important component of overall ecosystem restoration, especially for tropical islands from which they have been extirpated.
Downregulation of m6A writer complex member METTL14 in bladder urothelial carcinoma suppresses tumor aggressiveness
Authors: Guimarães‐Teixeira Catarina , Lobo João , Miranda‐Gonçalves Vera , Barros‐Silva Daniela , Martins‐Lima Cláudia , Monteiro‐Reis Sara , Sequeira José Pedro , Carneiro Isa , Correia Margareta P. , Henrique Rui , Jerónimo Carmen 
Published: January 2022

N6‐methyladenosine (m6A) and its regulatory proteins have been associated with tumorigenesis in several cancer types. However, knowledge on the mechanistic network related to m6A in bladder cancer (BlCa) is rather limited, requiring further investigation of its functional role. We aimed to uncover the biological role of m6A and related proteins in BlCa and understand how this influences tumor aggressiveness. N6‐adenosine‐methyltransferase catalytic subunit (METTL3), N6‐adenosine‐methyltransferase noncatalytic subunit (METTL14), protein virilizer homolog (VIRMA), and RNA demethylase ALKBH5 (ALKBH5) had significantly lower expression levels in BlCa compared to that in normal urothelium. METTL14 knockdown led to disruption of the remaining methyltransferase complex and a decrease in m6A abundance, as well as overall reduced tumor aggressiveness (decreased cell invasion and migration capacity and increased apoptosis). Furthermore, in vivo, METTL14 knockdown caused tumor size reduction. Collectively, we propose methyltransferase METTL14 as a key component for m6A RNA deposit and that it is closely related to BlCa progression, playing an important role in tumor aggressiveness. These data contribute to a better understanding of the m6A writer complex, which might constitute an appealing therapeutic target.
Evaluation of the quality of fit of flexible bolus material created using 3D printing technology
Authors: Malone Ciaran , Gill Elaine , Lott Tanith , Rogerson Catherine , Keogh Sinead , Mousli Majed , Carroll Denise , Kelly Caitriona , Gaffney John , McClean Brendan 
Published: January 2022

AbstractAimsTo retrospectively evaluate the quality of fit of 3D printed bolus over four different treatment sites to determine whether certain sites favor a 3D printed approach and if the quality of fit changes over the course of treatment.Materials and methodsA retrospective analysis of the first 60 cases treated using 3D printed bolus in our radiotherapy center was undertaken. All boluses were printed using flexible thermoplastic polyurethane (TPU) material. We developed a system of rating the quality of fit using four quality categories. The analysis of 60 patients consisted of a review of a total 627 treatment fractions for head and neck (H&N), scalp, pelvis, and extremity treatment sites.ResultsOut of 627 fractions evaluated, 75.1% were rated either “good” or “excellent”, 20.6% were rated as “acceptable” and 4.3% were rated “poor”. H&N, scalp, and extremity treatment regions were found to favor a 3D printed approach. However, pelvis cases had a higher proportion of “acceptable” and “poor” ratings. Trend analysis showed no notable change in the quality of 3D printed bolus fit over the course of treatment, except for pelvis cases which tended to change categories more than other treatment sites.ConclusionThis evaluation demonstrates that 3D printed bolus, created using semi‐flexible materials such as TPU, is an effective and practical bolus choice for radiotherapy. In particular, using a 3D printed approach for H&N, scalp, and extremities was found to have a highly conformal fit.
β‐Carotene—production methods, biosynthesis from Phaffia rhodozyma, factors affecting its production during fermentation, pharmacological properties: A review
Authors: Gupta Isha , Adin Syeda Nashvia , Panda Bibhu Prasad , Mujeeb Mohd. 
Published: January 2022

Abstractβ‐Carotene is the most treasured provitamin A carotenoid molecule exhibiting antioxidant and coloring properties and significant applications in the food, pharmaceutical, and nutraceutical industries. β‐Carotene has many biological functions within the human body; however, it is not synthesized within the human body, so its requirements are fulfilled through food and pharmaceuticals. Its manufacturing via chemical synthesis or extraction from plants offers low yields with excessive manufacturing expenses, which attracted the researchers toward microbial production of β‐carotene. This alternative provides higher yield and low expenses and thus is more economical. Phaffia rhodozyma is a basidiomycetous yeast that is utilized to prevent cardiovascular diseases and cancer and to enhance immunity and antiaging in people. This paper reviews the methods of production of β‐carotene, biosynthesis of β‐carotene fromP. rhodozyma, factors affecting β‐carotene production during fermentation, and pharmacological properties of β‐carotene.
Aerodynamische Belastungen auf bahntechnische Einbauten in schnell befahrenen Eisenbahntunneln
Authors: Reiterer Michael , Schellander Janez 
Published: January 2022

AbstractDie bei Zugfahrten in Eisenbahntunneln auftretenden druckwellen‐ und strömungsinduzierten aerodynamischen Belastungen werden dargelegt und in Bezug auf die Beanspruchungswirkungen der in modernen Bahntunneln ausgeführten unterschiedlichen Einbauten diskutiert. Es wird gezeigt, dass die in den derzeitigen Richtlinien und Normen angegebenen Belastungsgrößen tw. unvollständig sind und insbesondere die Effekte einer Wirbelablösung am Heck des fahrenden Zugs mit der daraus sich einstellenden hochgradig dynamischen Wirbelschleppe nicht abdecken. Im Zuge von mehreren Messkampagnen und begleitenden numerischen Simulationsberechnungen wurden in Österreich die in der Realität bei Zugfahrten in Eisenbahntunneln tatsächlich auftretenden aerodynamischen Belastungen abgeleitet und darauf aufbauend für unterschiedliche Einbauten die bei der Bemessung anzusetzenden Belastungswerte formuliert. Bei den Simulationsberechnungen wurde insbesondere auch die bei langen Tunneln maßgebende Fahrt von mehreren Zügen dicht hintereinander untersucht. Die Durchführung dieser numerischen Simulationsberechnungen und Messkampagnen unter realen Betriebsbedingungen in schnell befahrenen eingleisigen Eisenbahntunneln sowie die erzielten Ergebnisse werden dargelegt.
circKCNN2 suppresses the recurrence of hepatocellular carcinoma at least partially via regulating miR‐520c‐3p/methyl‐DNA‐binding domain protein 2 axis
Authors: Liu Donghong , Liu Wenbin , Chen Xi , Yin Jianhua , Ma Longteng , Liu Mei , Zhou Xinyu , Xian Linfeng , Li Peng , Tan Xiaojie , Zhao Jun , Liao Yong , Cao Guangwen 
Published: January 2022

AbstractBackgroundRecurrence is the major cause of hepatocellular carcinoma (HCC) death. We aimed to identify circular RNA (circRNA) with predictive and therapeutic value for recurrent HCC.MethodsTissue samples from recurrent and non‐recurrent HCC patients were subjected to circRNA sequencing and transcriptome sequencing. circKCNN2 was identified through multi‐omics analyses. The effects of circKCNN2 on HCC were evaluated in cells, animals, database of The Cancer Genome Atlas, and a cohort with 130 HCC patients. circRNA precipitation, chromatin immunoprecipitation assay, RNA pull‐down, luciferase assay, and cell experiments were applied to evaluate the interaction of circKCNN2 with miRNAs and proteins. The association between circKCNN2 and the therapeutic effect of lenvatinib was investigated in HCC cell lines and HCC tissue‐derived organoids.ResultsThe expression of circKCNN2 was downregulated in HCC tissues and predicted a favorable overall survival and recurrence‐free survival. The expression of circKCNN2 was positively correlated with the parental gene, potassium calcium‐activated channel subfamily N member (KCNN2). Nuclear transcription factor Y subunit alpha (NFYA) was proven to inhibit the promoter activity of KCNN2, downregulate the expression of KCNN2 and circKCNN2, and predict an unfavorable recurrence‐free survival. Ectopic expression of circKCNN2 inhibited HCC cell proliferation, colony formation, migration, and tumor formation in a mouse model. miR‐520c‐3p sponged by circKCNN2 could reverse the inhibitory effect of circKCNN2 on HCC cells and down‐regulate the expression of methyl‐DNA‐binding domain protein 2 (MBD2). The intratumoral expression of MBD2 predicted a favorable recurrence‐free survival. circKCNN2 down‐regulated the expression of fibroblast growth factor receptor 4 (FGFR4), which can be reversed by miR‐520c‐3p and knockdown of MBD2. Lenvatinib inhibited the expression of FGFR4 and upregulated the expression of circKCNN2 and MBD2. Ectopic expression of circKCNN2 in HCC cells enhanced the therapeutic effect of lenvatinib. However, the high inherent level of circKCNN2 in HCC cells was associated with lenvatinib resistance.ConclusionscircKCNN2, transcriptionally repressed by NFYA, suppresses HCC recurrence via the miR‐520c‐3p/MBD2 axis. Inherent level of circKCNN2 in HCC cells predisposes anti‐tumor effect of lenvatinib possibly because both circKCNN2 and lenvatinib repress the expression of FGFR4. circKCNN2 may be a promising predictive biomarker and therapeutic agent for HCC recurrence.
A transboundary political ecology of air pollution: Slow violence on Thailand's margins
Authors: Marks Danny , Miller Michelle Ann 
Published: January 2022

AbstractThis study develops a transboundary political ecology of air pollution to show how its spatially and socially unequal distribution constitutes a form of slow violence among already marginal sections of society. Recent research on transboundary air pollution in Southeast Asia and globally has mainly focused on the supranational or regional scale of environmental governance without taking into proper account the socially differentiated impacts of these cross‐border flows of environmental harm at lower organisational scales. Air pollution in Thailand, which ranks amongst the worst in the world, generates spill‐over effects across sub‐national borders that disproportionately impact the urban and rural poor. We examine the drivers of the three major sources of air pollution in Thailand: vehicular emissions, agricultural emissions and industrial emissions to direct attention toward the barriers and opportunities for collaborative governance in urban, peri‐urban and rural settings. The article argues that administrative fragmentation and the protection of vested economic interests by Thai business and political elites have compromised transboundary governance of the air while adding to socio‐spatial inequalities and environmental injustices. We recommend legislative reforms centred on cross‐sectoral and cross‐jurisdictional cooperation to provide redress for the slow violence perpetrated against marginal citizens in the governance of air pollution.
Curriculum mapping of health promotion competencies in dental and oral health training programmes in Australia
Authors: Bracksley‐O’Grady Stacey , Anderson Karen , Dickson‐Swift Virginia , Masood Mohd 
Published: January 2022

AbstractIssue addressedDental diseases are chronic conditions that place a significant burden on the population's health; however, they are mostly preventable using a range of health promotion strategies Health promotion is a core competency for all dental and oral health graduates, but little is known about what health promotion content is taught in undergraduate degrees. The aim of this study was to explore the dental and oral health content in Australian undergraduate dental and oral health degrees and map against the last two versions of the Australian Dental Council (ADC) health promotion competencies.MethodsAll ADC‐accredited dental and oral health courses delivered at Australian universities in 2019 were eligible to be included. Key words were used to locate subjects within the courses that contained health promotion content. This was analysed and ranked against the last two versions (2011 and 2016) of the ADC health promotion competencies. The competencies were then ranked using Blooms updated “six levels of thinking.”ResultsSeven oral health and eight dental courses were mapped. The number of health promotion subjects in courses varied substantially; the percentage of subjects that contained health promotion in oral health courses ranged from 30% to 75% and 16% to 60% for dental courses. All oral health courses were explicitly meeting the current ADC health promotion competencies, however, only half of the dental courses met the competency standards.ConclusionCurriculum mapping provided a snapshot of the health promotion content within dental and oral health degrees in Australia. Evaluations of the extent to which these courses meet the ADC competencies provide useful information for students, clinicians and policy makers.So What?This study provides evidence that health promotion training is occurring at varying levels. However, in dentistry, not all the ADC health promotion competencies are being met. A change is needed in the ADC health promotion competencies and ethos of academics involved in the development of curriculum to include and give appropriate attention to health promotion theory, especially advocacy. This will enable future dental professionals to advocate for a range of oral health promotion activities such as water fluoridation, universal dental care and sugar‐sweetened beverage taxes.
microRNA‐1321 and microRNA‐7515 contribute to the progression of non‐small cell lung cancer by targeting CDC20
Authors: Hu Hao , Tou Fang‐Fang , Mao Wei‐Min , Xu Yan‐Liang , Jin Hui , Kuang Yu‐Kang , Han Chun‐Bin , Guo Chang‐Ying 
Published: January 2022

AbstractCell division cycle 20 (CDC20) and microRNAs (miRNAs) are differentially expressed in non‐small cell lung cancer (NSCLC). The current study aimed to investigate the role of miR‐1321 and miR‐7515 regulation in CDC20 during NSCLC development. CDC20 expression in paracancerous and tumor tissues was assessed using quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). The relationship between CDC20 expression and prognosis of patients was analyzed using the TCGA database. The expression profile of CDC20 in healthy lung cells and NSCLC cells was detected using qRT‐PCR and western blotting. After the knockdown of CDC20 in NSCLC cells, the cell proliferation, apoptosis, migration, invasion, and cell cycle changes were investigated by CCK8, EdU, flow cytometry, wound healing, and Transwell assays. The miRNAs targeting CDC20 were predicted using two bioinformatics websites and validated using dual‐luciferase assays. CDC20 was enhanced in NSCLC tissues and cells, thus predicting the poor prognosis in NSCLC patients. After CDC20 inhibition, the malignant phenotype of NSCLC cells was reverted. miR‐1321 and miR‐7515 targeted CDC20 and exhibited the same anti‐tumor effects as CDC20 silencing. Functional rescue experiments showed that CDC20 overexpression averted the anti‐tumor effects of miR‐1321 and miR‐7515 on NSCLC cells. miR‐1321 and miR‐7515 inhibited NSCLC development by targeting CDC20. Thus, the current study has implications in NSCLC treatment and provides novel insights into NSCLC management.