bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibitory Effect of Baicalin on 2019-NCOV Invasion
Authors: Geng Xue , Zhou Meng , Sun Rong , Wei Xia , Zhu Qing-fen 
Published: January 2022

Abstract
Objective To study the the inhibitory effect of baicalin on novel Coronavirus (2019-NCOV) entry process in vitro. Methods The pseudovirus system of SARS-CoV-2 S protein constructed with luciferase reporter gene was used in the study. A luciferase kit was used to detect the changes of luciferase expression in Huh-7 cells, and then the virus inhibition curve was plotted. Results Baicalin can significantly inhibited the infection rate of pseudovirus. There was no significant difference in the virus inhibition curve between the baicalin&virus pre-incubation group and co-incubation at different concentrations, indicating that baicalin could not directly bind to virus, but inhibited the virus S protein mediated cell fusion process. We futher found that, the inhibition rate of baicalin to virus decreased significantly in the 4h group, but had no significant difference in the 0h and 2h groups at the concentration of 0.125mg/ mL, indicating that baicalin may have an inhibitory effect on virus invasion stage rather than adsorption stage, and the mediated inhibition stage occurred within 4h. Conclusion Baicalin may mediate the fusion of SARS-CoV-2 S protein with cell surface receptor and exert anti-novel coronavirus activity by playing a role in inhibitting virus invasion in non-adsorption stage.
Beyond 10 MHz Organic Electrochemical Transistors for Printed Multi-valued Logic Circuits
Authors: Lim Dong Un , Jo Sae Byeok , Cho Jeong Ho 
Published: January 2022

Abstract
Printable electronics requires solution-processable transistors that can perform high-speed operations with high gains, with which its applicability can embrace bio-interfacing, neuromorphic computing, and even high-functioning logical circuitry. Although organic electrochemical transistors (OECTs) with superior transconductance and biocompatibility have emerged as feasible candidates, the intrinsic coupling between ionic and electronic carriers leads to limited response times falling behind other transistor technologies. Here, we overcome such limitations by introducing a vertically stacked dual-channel architecture enabling an unprecedentedly high operation speed with multiple logic states. Specifically, a simple implementation of dual-ion penetration mechanisms allows facile manifestations of stable and equiprobable ternary logic states, to which state-of-the-art accessing frequency over 10 MHz is realized. Furthermore, printed arrays of ternary logic gates with full voltage swing within 1V is demonstrated.
Fire and water: global fire impacts on physicochemical properties of freshwater ecosystems
Authors: Morales Jenny J. , Paes Nayara D. S. , Silva Andressa C. M. , Teixido Alberto L 
Published: January 2022

Abstract
Freshwater ecosystems are intensely endangered, entailing declines in the biodiversity and ecosystem services. Fire may affect physicochemical water quality parameters of these environments by transference of ash and toxic substances into water bodies, altering nutrient and oxygen concentrations, temperature or turbidity. However, major knowledge gaps in the impacts of fire on freshwater habitats are prevalent. We conducted a global systematic review by searching articles in the Web of Science related to the impacts of fire on the physicochemical water properties of freshwater ecosystems. We estimated ecological and geographic biases, quantified the set of physicochemical parameters investigated, and provided an overview of fire-mediated alterations. We found 54 articles published between 1976 and 2019, with a significant increase during the last decade. Research was strongly biased towards a few western countries, mostly across North America, Europe and Australia. Lotic systems were considerably more studied than lentic systems. Overall, 57 chemical and 17 physical parameters were evaluated, comprising 304 and 77 cases, respectively. Fire altered 68% of the cases studied and this alteration was proportionally similar between chemical (66%) and physical (75%) properties. Nutrient concentration, metal ions, alkalinity, turbidity and temperature were recurrently increased parameters by fire, whereas oxygen mostly decreased. Our study demonstrates that fire commonly affects water quality parameters of freshwater ecosystems. Biases and resulting gaps may steer future sampling efforts and promote a broader understanding of the impacts of fire on these environments. Our results may ultimately guide applicable conservation policies for biodiversity and water management strategies of freshwater habitats.
Computational investigation of pyrrolidin derivatives as novel GPX4/MDM2–p53 inhibitors using 2D/3D-QSAR, Molecular docking, Molecular dynamics simulations and MM-GBSA free energy
Authors: TABTI Kamal , SBAI Abdelouahid , ELMCHICHI Larbi , MAGHAT Hamid , LAKHLIFI Tahar , BOUACHRINE Mohammed 
Published: January 2022

Abstract
The p53 is a tumor suppressor protein that adjusts cell cycle and growth arrest as well as genes that restore DNA damage and apoptosis. Murine double minute 2 (MDM2) is a main p53 antagonist. We created a novel QSAR model using a series of highly active spiro [pyrrolidin-3,2-oxindoles] that consisted of 29 compounds that were experimentally validated to inhibit the MDM2-p53 interaction. Three optimal models have been developed CoMFA/E+S, CoMSIA/S+H+A and HQSAR have revealed good statistical results, but the CoMSIA mode only which validates all the external validation tests applied successfully. Based on the CoMSIA/S+H+A model was carefully chosen to design four compounds with values ​​of inhibitory activity greater than the highly active compound in the data set. The Newly designed compounds were docked in the target receptor binding site (ID: 4LWU). The newly designed compound Pred 01 showed the highest affinity with a value of -9.4kcal / mol, while compound N°04 which represents the data set and control compound (Nutlin-3) showed binding energies of the order of -8.8 kcal / mol and -8.2kcal / mol, respectively. In addition, the roles of lipinski and veber were estimated, the results obtained demonstrate that the proposed molecules involve good oral bioavailability and an ability to diffuse through different biological barriers. For in-depth study, The Pred01 / receptor, N°04 / receptor and Nutlin-3 / receptor complexes were selected via dynamic simulation analyzes with a simulation time of 100 ns and, also their free binding energy was examined operating the MM-GBSA approach. The molecular docking results obtained accentuate the crucial residues responsible for the ligand / protein interaction, providing insight into the mode of interaction. The MD simulation analysis confirms the conformational stability of the selected complexes during the MD trajectory, and the fluctuations recorded are insignificant. The results of MM-GBSA reveal that the new compound Pred 01 exhibits the lowest free energy, which confirms the result of molecular docking.
Cytotoxicity and anticancer drug release behavior of Methionine - coated magnetite nanoparticles as carriers in vitro
Authors: Yeganeh Faten Eshrati , Yeganeh Amir Eshrati , Far Bahareh Farasati , Akbarzadeh Iman , Quazi Sameer , Safdar Muhammad 
Published: January 2022

Abstract
An innovative and customized drug delivery system for in vitro cancer treatment has been developed successfully by a simple one-step method. A CoFe2O4@Methionine core-shell nanoparticle was prepared by the reflux assay, in which amino acid on the surface makes the ferrite biocompatible, enhances the chemical stability of the compound, and increases the drug loading capacity. The synthesized nanoparticles were evaluated using SEM, TEM, FTIR, and VSM, while XRD and TGA analysis verified the presence of a coating amino acid on the surface of CoFe2O4. The appearance of a new peak for C≡N in the FTIR spectrum validates the synthesis of a letrozole-loaded carrier. Both uncoated CoFe2O4 and methionine-coated CoFe2O4 nanoparticles behave super-paramagnetically at room temperature, with saturation values of 46 emu/g and 16.8 emu/g, respectively. SEM and TEM were used to characterize the morphology and size of samples, revealing that the average particle size was around 28–29 nm. The loading of Letrozole and the effect of pH (5, 7.4) on the release behavior of the carrier were studied. The result of the drug release in pH (5) was about 88% higher than pH (7.4). Also, the preparation has been evaluated for determining its cytotoxicity using MCF-7, MDA-MB-231, and MCF10A cell lines as an in vitro model, and the results of in vitro experiments showed that CoFe2O4@Methionine could significantly reduce cancer in the cell model. These results demonstrate that core-shell nanoparticles were prepared that are biocompatible and have potential use as drug delivery.
The Long-Term Impact of Covid-19 On Presentations To A Specialist Child &amp; Adolescent Eating Disorder Program
Authors: Campbell Sally , Maunder Kristen , Lehmann Oonagh , McKeown Mark , McNicholas Fiona 
Published: January 2022

Abstract
Background: Concern has been expressed about an increase in mental illness and eating disorders in particular during the pandemic. Whether Covid-19 associated stress presents a specific risk for eating disorders, new or relapsing, in vulnerable groups remains to be established. Aim: This study explores any impact of Covid-19 on numbers and clinical profile of eating disorder presentations to a Child and Adolescent Mental Health Service (CAMHS) specialist eating disorder treatment program pre and during Covid-19. Methods: Following ethical approval, retrospective data extraction from electronic healthcare records from all referrals to a CAMHS specialist eating disorder treatment program in Dublin, Ireland was conducted. The study period covered 42months, from 1st January 2018 until 31st Aug 2021. Referral rates and clinical characteristics of pre- Covid-19 (January 2018-Feb 2020) and Covid-19 patients (March 2020-Aug 2021) were examined, using Welch’s t- and chi-square tests for continuous and categorical data respectively. Results: 128 youth were assessed over the 42-month study period, with significantly higher rates of referrals post-pandemic (3.78 per month compared to a pre- Covid-19 rate of 2.31/month). (p=.02). Youth referred during Covid-19 lost weight at a faster rate; 2.3kg/month compared to 1.9kg/month pre- Covid-19 (p=.39) and showed a higher rate of % Ideal bodyweight loss (IBW), 4.8% IBW/month compared to 2.6% IBW/month pre- Covid-19 (p=<.001). They also had a shorter duration of illness pre-referral (M=7.4 months, SD=3 pre-Covid-19; M=4.8 months, SD=4.5 post Covid-19, (p<.001), with fewer youth (19%) prescribed psychotropic medication than pre Covid-19 (43%, p=.011). Many youth (80%) self-declared Covid-19 as having had an adverse effect on their overall well-being and as a contributory factor to the development of their eating disordered pathology.Conclusions: This study supports the growing consensus of a Covid-19 specific impact for ED services with higher rates of referrals, youth presenting with a faster pace of IBW% loss and earlier referral to specialist services. Whether this reflects a true and specific increase in EDs or reflects an overall increase in CAMHS referrals, with increased transfer to ED services, needs further examination.
A multiomics signature highlights alterations underlying homologous recombination deficiency in triple-negative breast cancer
Authors: You Chao , Su Guan-Hua , Jiang Lin , Xiao Yi , Zheng Ren-Cheng , Wang He , Jiang Yi-zhou , Peng Wei-jun , Shao Zhi-Ming , Gu Ya-Jia 
Published: January 2022

Abstract
Background: Homologous recombination (HR) is a key pathway in DNA double-strand damage repair. HR deficiency (HRD) more commonly occurs in triple-negative breast cancers (TNBCs) than in other breast cancer subtypes. Several clinical trials have demonstrated the value of HRD in stratifying breast cancer patients into distinct response groups to poly ADP ribose polymerase (PARP) inhibitors and chemotherapy.Methods: We retrospectively collected TNBC samples to establish a multiomics cohort (n=343) to explore the biological and phenotypic mechanisms underlying the better prognosis of patients with high HRD scores. The HRD score was calculated using the sum of the telomeric allelic imbalance (TAI) score, loss of heterozygosity (LOH) score and large-scale state transition (LST) score. Gene set enrichment analysis (GSEA) were conducted to reveal underlying pathways of low HRD score patients. Radiomics model were established to predict HRD score in a noninvasive method.Results: Multivariable Cox analysis revealed the independent prognostic value of a low HRD score (hazard ratio, 2.20, 95% confidence interval, 1.05 to 4.59, p=0.04). Furthermore, amino acid and lipid metabolism pathways were highly enriched in tumors with low HRD scores, which was also demonstrated by differentially abundant metabolite analysis. A noninvasive radiomics method was developed to predict HRD status and performed well in the independent validation cohort (support vector machine model: area under the curve (AUC), 0.739, sensitivity, 0.571, and speciﬁcity, 0.824; logistic regression model: AUC, 0.695, sensitivity, 0.571, and speciﬁcity, 0.882).Conclusions: In conclusion, we revealed the prognostic value of the HRD score, predicted HRD status with noninvasive radiomics features and preliminarily explored druggable targets in TNBC patients with low HRD scores.
Tigecycline Induced Fibrinogen Decrease in Critically Ill Patients with Multidrug Resistant Bacterial Infections - an Observational Retrospective Study
Authors: Heng Junfeng , Zhang Xiuhong , Liang Fengming , Wang Qiuhui , Xu Hongyang , Hui Jiaojie 
Published: January 2022

Abstract
A decrease of plasma fibrinogen level has occasionally been observed during tigecycline treatment. The present observational retrospective study was performed to analyze the effect of tigecycline on coagulation function in critically ill patients with multidrug-resistant bacterial infections, especially on plasma fibrinogen level. 83 adult subjects treating with tigecycline were enrolled. Coagulation function spanning all three time periods (berore, during and after tigecycline administration) was determined to measure the effects of tigecycline. A striking decrease of fibrinogen was noted during tigecycline treatment (P=0.000), and this change started to reverse when tigecycline was stopped. In the higher-dose group, fibrinogen also dropped significantly during tigecycline treatment (P=0.009), and after the cessation of tigecycline, the fibrinogen level did not rebound, but kept on drop. The fibrinogen levels dropped significantly in the 4 patients developed new-onset bleeding during tigecycline treatment, showing almost simultaneously. There were increased prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), platelet count (PLT) and decreased platelet level during tigecycline treatment and a reversal when tigecycline was stopped, although only the difference of PT reached significance (P=0.043). Tigecycline induced fibrinogen decrease in critically ill patients with multidrug-resistant bacterial infections, and this change started to reverse when tigecycline was stopped.
Women’s Perceptions About Mobile Health Solutions for Selection and Use of Family Planning Methods in Karachi: A Feasibility Study
Authors: Abrejo Farina , Iqbal Romaina , Saleem Sarah 
Published: January 2022

Abstract
Background

The qualitative study explored the feasibility and acceptability of potential mHealth intervention from women living in low socio-economic areas to increase the uptake of family planning. The study also discussed the potential benefits of mHealth intervention by exploring providers perceptions as well.
Methods

The qualitative exploratory study recorded the perception of 23 women and seven in-depth interviews with the providers of family planning services. These interviews asses women’s attitude though; personal experience based on the usage of smartphones and family planning, acceptability for personal benefits, features of mobile application and the convenient language, and self-efficacy for identifying the potential impact of mHealth intervention to increase women’s empowerment for family planning usage.
Results

Three predetermined themes were used to record the perception of women.The personal experience of women identified that women in low-socio economic areas use mobile phone frequently, they use phone to get preventive information for healthcare. Few women have experience using mobile phones for getting information about sexual and reproductive health. Women considered; poor counselling and high transport costs to the facilities, as major barriers to getting family planning services. Perceived acceptability discussed the potential features of the mHealth app. Women considered including short videos instead of written material will help them to understand its usage, complete information about family planning method including; dosage, expected side effects and potential benefits suggested to include in the app. Women perceived that the mHealth intervention will save women’s cost of transport to the facility and will fill the information gap about family planning methods. While providers considered that it will save their time in counselling and motivating women for the method at the facility. Self-efficacy in the form of women empowerment in deciding family planning was one of the major factors discussed, where women discussed that the mHealth intervention will increase their self-confidence to discuss the method with their husbands.
Conclusion

There is a high potential for mHealth interventions for family planning in Pakistan. The usage of mobile phones can be utilized for increasing women’s acceptability and accessibility for family planning uptake in the country.
Psychometric Properties and Validation of the English Version Giessen Subjective Complaints List (GBB-8)
Authors: Petrowski Katja , Zenger Markus , Schmalbach Bjarne , Bastianon Christina Diane , Strauss Bernhard 
Published: January 2022

Abstract
Background
The present study investigated the psychometric properties of the newly developed English version of the Giessen Subjective Complaint List – 8 (GBB-8), a questionnaire assessing psychosomatic symptoms with regard to exhaustion, gastrointestinal, musculoskeletal and cardiovascular.
Methods
A U.S. sample of 638 participants (47.6% female) was recruited by MTurk to participate in this cross-sectional online survey. Validation instruments included the Patient Health Questionnaire-4, Perceived Stress Scale, short version of the Trier Inventory for Chronic Stress.
Results
Reliability was high with ω’s between .80 and .86 for all subscales. Confirmatory factor analyses yielded comparable good model fit for a four-dimensional model as well as a higher order model. Multi-group confirmatory factor analyses confirmed measurement invariance of the GBB-8 across sex and age. Regarding convergent validity, correlations with other instruments were highly significant and of large magnitude as expected.
Conclusion
The English version of the GBB-8 has shown excellent psychometric properties. Therefore, it can be recommended for the assessment of psychosomatic complaints in contexts where short screening instruments are necessary.
A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21
Authors: Dema Anusha , Tallapally Mounika , Ganji Vishweshwar Kumar , Buddala Bhagyalakshmi , Kodi Haritha , Ramidi Ashwini , Chenji Sai Spandana , P Jaya Laxmi , S Vamsi Krishna , Yella Narasimha Reddy , Putty Kalyani 
Published: January 2022

Abstract
Viral pathogens account for major aetiology of canine gastroenteritis in India. However, till date, there was no structured comprehensive study reported on the viral aetiology of canine gastroenteritis in India. To elucidate this, 475 rectal swabs collected over a period of three years (2018-2021) from clinical canine gastroenteritis cases were screened by real-time PCR for the presence of six majorly known enteric viruses (Canine parvovirus -2 (CPV-2), canine distemper virus (CDV), canine adenovirus-2 (CAdV-2), canine corona virus (CCoV), canine astrovirus (CaAstV), canine rotavirus (CRV)). 71.6% of the samples were found to harbour at least one of the five viruses tested (CPV-2/2a/2b/2c, CDV, CAdV-2, CCoV and CaAstV), whereas, there was no evidence of CRV. The overall incidence rate for each virus was found highest at 64.8% for CPV-2/2a/2b/2c (2a: 21.1%, 2b: 77.4%, 2c: 1.5%), followed by 8% for CDV, 7.2% for CaAstV, 5.9% for CCoV and 4.6% for CAdV-2 with 16.8% incidence for coinfections ranging from two to four viruses in different combinations. Further, for the first time we report the whole genome sequences of CDV, CCoV and CaAstV Indian isolates from canine gastroenteritis cases. Phylogenetic analysis of whole genome revealed that CDV clustered with Asia-1 lineage, CaAstV clustered with group III that consists of China and Brazil isolates, and CCoV clustered with group II that consists of China and Taipei isolates. This study adds to our knowledge on the prevalence of these pathogens in the Indian subcontinent and highlights the need for relevant and routine vaccine prophylactic measures. It is alarming to note that 70.3% of animals vaccinated with DHPPIL were found positive for at least one virus. Hence, regular molecular analysis of the prevalent viruses is crucial to address vaccination failures.
Utilization of Facility-based Delivery Services in the Wa Municipality, Ghana
Authors: Nuolabong Culbert , Akuffo Kwadwo Owusu , Kabiri Mukasa , Nortey Priscillia , Maya Ernest Tei , Junior Isaiah Osei Duah , Danso-Appiah Anthony 
Published: January 2022

Abstract
Background: Access to skilled delivery services are crucial in reducing maternal mortality, however, the prevalence of women with assisted deliveries in our health facilities remains low with reasonable disparities between rural and urban Ghana. This study examined the utilization of skilled delivery by pregnant women and its associated factors in the Wa Municipality of Ghana.Methods: The study employed a community-based cross-sectional study. A simple random sampling was used to recruit two rural and two urban sub-districts and subsequently four communities selected from each of the respective sub-districts. Thirty-three eligible women of reproductive age (15- 49 years) who had delivered within the last one-year prior to the commencement of the study were selected from each community. A structured questionnaire was used to collate information on respondents socio-demographic, geographic and cultural factors that affect the utilization of skilled delivery services. Data was analyzed using Stata Statistical Software (version 12), frequencies and percentages were used to summarize data and associations between variables investigated with multiple regression analyses at a significance of p<0.05.Results: Out of the 527 women interviewed, 481 (91.6%) had a skilled delivery. Mothers were aged between 15 and 45 years with mean (± SD) age of 26.4 ± 9.4 years. After statistical adjustment, rural residence (AOR=2.7, 95% CI 0.8 – 8.9, p=0.036 compared with urban residence), never attended antenatal care (AOR=101, 95% CI 10.2 – 1017, p<0.001), health facility delivery (AOR=0.1, 95% CI 0.1 – 0.6, p<0.001), time taken to nearest health facility (≥31 minutes AOR=11.7, 95% CI 3.6 – 38.6, p<0.001), and no Husband’s consent (AOR=4.6, 95% CI 1.8 – 11.6, p<0.001) were significantly associated with uptake of skilled delivery services.Conclusion: The findings of this study demonstrate adequate utilization of skilled delivery services among women in the Municipality. Efforts towards improvement of skilled delivery coverage should focus on health education, especially among rural women together with the expansion of healthcare services.