bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recapitulation of pro-inflammatory signature of monocytes with ACVR1 mutation using FOP patient-derived iPSCs.
Authors: Maekawa Hirotsugu , Jin Yonghui , Nishio Megumi , Kawai Shunsuke , Nagata Sanae , Kamakura Takeshi , Yoshitomi Horoyuki , Niwa Akira , Saito Megumi , Matsuda Shuichi , Toguchida Junya 
Published: May 2022

Abstract
Background Fibrodysplasia ossificans progressiva (FOP) is a rare genetic disease characterized by progressive heterotopic ossification (HO) in soft tissues due to a heterozygous mutation of the ACVR1A/ALK2 gene (FOP-ACVR1A), which erroneously transduces the BMP signal by Activin-A. Although inflammation is known to trigger HO in FOP, the role of FOP-ACVR1A on inflammatory cells remains to be elucidated. Results We generated immortalized monocytic cell lines from FOP-iPSCs (FOP-ML) and mutation rescued iPSCs (resFOP-ML). Cell morphology was evaluated during the monocyte induction and after immortalization. Fluorescence-activated cell sorting (FACS) was performed to evaluate the cell surface markers, CD14 and CD16, on MLs. MLs were stimulated by lipopolysaccharide (LPS) or Activin-A and the gene expression was evaluated by quantitative PCR (qPCR) and microarray analysis. Histological analysis was performed for HO tissue obtained from wild type mice and FOP-ACVR1 mice which conditionally express human mutant ACVR1 gene by doxycycline (Dox) administration.Without any stimulation, FOP-ML showed the pro-inflammatory signature of CD16+ monocytes with upregulation of INHBA gene, and treatment of resFOP-ML with Activin-A induced the expression profile mimicking those of FOP-ML at baseline. Treatment of FOP-ML with Activin-A further induced the inflammatory profile with up-regulation of inflammation-associated genes, some, but not all, of which were suppressed by corticosteroid. Experiments using an inhibitor for TGFβ or BMP signal demonstrated that Activin-A-induced genes such as CD16 and CCL7 were regulated by both signals, indicating Activin-A transduced dual signals in FOP-ML. Comparison with resFOP-ML identified several down-regulated genes in FOP-ML including LYVE-1 , which is known to suppress matrix-formation in vivo. Down-regulation of LYVE-1 in HO tissues was confirmed in FOP model mice, verifying the significance of in vitro experiments. Conclusion These results indicate that FOP-ML faithfully recapitulated the phenotype of primary monocytes of FOP and the combination with resFOP-ML is a useful tool to investigate molecular events at the initial inflammation stage of HO in FOP.
Antifouling Activity of Isonitrosoacetanilides Against Micro and Macrofouling
Authors: Agostini Vanessa Ochi , Martinez Sabrina Teixeira , Muxagata Erik , Macedo Alexandre José , Pinho Grasiela Lopes Leães 
Published: May 2022

Abstract
Biofouling is responsible by structural and economic damage on man-made surfaces. Antifouling paints with biocides had been applied in these structures to avoid organisms adhesion, however they show high toxicity and they are not able to avoid all biofouling process, necessitating the mechanical removal of organisms periodically and paint reapplication. Thus, there is an urgent demand for novel, effective, and environmentally friendly antifouling alternative. As isonitrosoacetanilide is the precursor for many compounds with antibacterial activity, we believe that it could show an antifouling activity potential against microfouling and per consequence also against macrofouling. The aim of this work was to investigate the antifouling potential of six isonitrosoacetanilides compounds and their toxicity. The compounds were employed in different concentrations (0.625–1.25–2.5–5–10 µg mL-1) in this study. The biofilm and planktonic bacteria inhibition and biofilm eradication potential were evaluated through by crystal violet assay, while Amphibalus amphitrite barnacle was evaluated by cyprid settlement assay. Toxicity evaluation (LC50 and EC50) was performed with A. amphitrite nauplii II and cyprid larvae. 4-Br-INA, 4-CH3-INA, and 2-Br-INA compounds, at least one of the tested concentrations, showed non-toxic antifouling activity against microfouling (antibiofilm) and macrofouling (anti-settlement). However, only 4-CH3-INA and 2-Br-INA showed also biofilm eradication potential. These compounds with antibiofilm activity and non-toxic effects could be combined with acrylic base paint resin or added directly into commercial paints in place of toxicant biocides to cover artificial structures as friendly antifouling.
Role of hypoxia-related genes in breast cancer based on a comprehensive analysis of scRNA-seq and bulk RNA-seq
Authors: Sun Rui , Yin Wenming , Zha Yilin , Xi Dan , Shao Yingjie , Gu Wendong , Jiang Jingting 
Published: May 2022

Abstract
Background: The hypoxic state in tumor microenvironment of breast cancer favors the proliferation and metastasis of tumor cells thus affecting patient survival. In this study, we aimed to combine single-cell sequencing data and bulk sequencing data to construct hypoxia-related prognostic signature of breast cancer patients.Methods:Single-cell RNA transcriptome data of MCF7 cells subjected to hypoxia microenvironment, the bulk tumour transcriptome data and clinical data were loaded from Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database. Screening for differentially expressed genes (DEGs) in MCF7 cells under hypoxic microenvironment. Functional enrichment analysis of these DGEs was performed via Gene ontology annotation (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Variation Analysis (GSVA). Univariate, the least absolute shrinkage and selection operator (LASSO) regression algorithm and multivariate Cox regression were applied to determine the prognostic gene signature. Risk score and clinical features were used to construct a nomogram model. 22 immune cell infiltrates were calculated using the CIBERSORT algorithm and correlations between risk scores and tumor microenvironment were analyzed. Results:Total 329 DEGs between the hypoxia and normoxia state were identified from scRNA-seq data. GO, KEGG and GSVA analysis revealed that the most gene sets were related to hypoxia. Combined with information of 1099 breast cancer samples sourced from the TCGA database, we identified four genes (ERRFI1, HSPB8, PGK1, STC2) to be independent prognostic genes, and risk scores based on their gene expression were calculated for each patient. Kaplan-Meier survival analysis showed a negative correlation between risk score and patient survival time. The validation using GSE20685 dataset data also yielded similar results. Risk scores and clinical data were considered as independent prognostic factors for breast cancer patients for the construction of a nomogram model, and the model showed good prognostic power. CIBERSORT algorithms was used to calculate 22 immune cell infiltrates in BRCA patients, and the analysis demonstrated that risk scores were positively associated with immunosuppression.Conclusions:In summary, we used single-cell sequencing data from MCF-7 cells under hypoxic conditions to identify prognosis-related genes, and the constructed prognostic model displayed well predictive properties.
Risk of hepatitis B virus reactivation in patients with resolved infection on therapy with corticosteroids and conventional synthesis immunosuppressants for kidney disease — a single-centre analysis of 258 patients
Authors: Han Pingyang , Wang Ziqiu , Wang Zhaohui , Shen Pingyan , Zhang Wen , Ren Hong 
Published: May 2022

Abstract
Background
The risk of hepatitis B virus (HBV) reactivation in patients with a previously resolved HBV (prHBV) infection on therapy with corticosteroids and conventional synthesis immunosuppressants (csISs) for kidney disease has not been well described.
Methods
We performed a retrospective study on the risk of HBV reactivation (HBVr) in patients with a prHBV infection on therapy with corticosteroids and csISs for kidney disease between January 2012 and December 2021 in the Department of Nephrology at Ruijin Hospital.
Results
A total of 258 patients with a prHBV infection (all treated with high-dose corticosteroids, of whom 192 were receiving a corticosteroid combined with csISs therapy, including cyclophosphamide (155), cyclosporine A (14), mycophenolate mofetil (14), and tacrolimus (9)) were enrolled. During a mean follow-up time of 21.66 months (range: 9–70 months), HBVr were not observed in these patients.
Conclusions
Among patients with a prHBV infection on therapy with corticosteroids and csISs for kidney disease, HBVr was not common and severe, suggesting that universal prophylaxis may not be justified or cost-effective in this clinical setting.
Approximation-free control based on the bioinspired reference model for suspension systems with uncertainty and unknown nonlinearity
Authors: Hu Xiaoyan , Wen Guilin , Yin Shan , Tan Zhao , Pan Zebang 
Published: May 2022

Abstract
Uncertainty and unknown nonlinearity are often inevitable in the suspension systems, which were often solved using fuzzy logic system (FLS) or neural networks (NNs). However, these methods are restricted by the structural complexity of the controller and the huge computing cost. Meanwhile, the estimation error of such approximators is affected by adopted adaptive laws and learning gains. Thus, in view of the above problem, this paper proposes the approximation-free control based on the bioinspired reference model for a class of uncertain suspension systems with unknown nonlinearity. The proposed method integrates the superior vibration suppression of the bioinspired reference model and the structural advantage of the prescribed performance function (PPF) in approximation-free control. Then, the vibration suppression performance is improved, the calculation burden is relieved, and the transient performance is improved, which is analyzed theoretically in this paper. Finally, the simulation results validate the approach, and the comparisons show the advantages of the proposed control method in terms of good vibration suppression, fast convergence, and less calculation burden.
Superdense state of the monolayer hydrogen on adsorbent under liquefied temperature
Authors: Gi Hiroyuki , Kashiwara Yuki , Itoh Yuki , Sharma Khushbu , Ogita Norio , Miyaoka Hiroki , Ogawa Tomofumi , Simanullang Marolop , Prost Laurent , Ichikawa Takayuki 
Published: May 2022

Abstract
A systematic examination of the cryogenic H2 adsorption properties below critical point of H2 (Tc = 33 K) on a various kind of adsorbents was carried out, then the density of adsorbed H2 on each adsorbent and its temperature dependence were experimentally compared with the liquid H2. Usually, H2 adsorption on porous materials was investigated at liquid nitrogen temperature (77 K) to realize its practical application for H2 storage and most of reports have focused on the development of new porous materials. On the other hand, this work was focused for the first time on the cryogenic H2 adsorption properties of metal organic frame works, superactivated carbon, and graphene nanoplatelets. We found superdense H2 adsorption as the monolayer state, having much higher density than liquid H2, which is contrary to the general understanding that the upper limit of adsorbed H2 density is believed to the density of liquid H2.
The mechanism of magnesium isoglycyrrhizinate in regulating the inflammatory microenvironment of gastric cancer
Authors: Miao Yuqing , Zhou Wenli , Wei Kunchen , Dong Fangyuan , Tang Yuan , Jiang Chang , Wang Jiejun 
Published: May 2022

Abstract
Background:
To determine the mechanism of magnesium isoglycyrrhizinate in regulating the inflammatory microenvironment to antagonize gastric cancer.
Methods:
We established MFC xenograft mouse models to investigate the effects of magnesium isoglycyrrhizinate on gastric cancer. A PCR Array chip was deployed to detect the expression level of 96 genes associated with inflammation and immune response. We used immunohistochemistry to stain the key immune cells to observe the density changes in gastric cancer inflammatory microenvironment. The possible mechanism was further investigated in Raw264.7 cells fed with conditioned media from MFC, the expression of M1 and M2 markers were tested by Western blot and ELISA.
Results:
Magnesium isoglycyrrhizinate reduced the tumor weight, volume and improved survival rate of gastric cancer xenograft mice. From the results of chip screening, changes in inflammation and immune response regulation factors included chemokines and receptors (CXCL1, CXCL2, CXCR1), interleukins and receptors (IL-1β, IL10, IL23A, IL2, IL12, IL1R1), TNF-α and key molecules in NF-κB pathway. Magnesium isoglycyrrhizinate induced the aggregation of CD8 + T cells, reduced Treg infiltration and increased the density of macrophages. M2 macrophage marker Arg1 and cytokines such as IL6, IL10, VEGF-A were down-regulated, on the contrary, the expression of M1 markers iNOS and cytokines IL -12 were up-regulated.
Conclusions:
Magnesium isoglycyrrhizinate can regulate the inflammatory microenvironment to enhance anti-tumor immunity and suppress immune escape in gastric cancer. Most importantly, it can induce macrophage differentiation into M1 probably through the NF-κB pathway in the cancer inflammatory microenvironment.
Does the ultrasonic activation of calcium silicate-based sealers affect their physicochemical properties?
Authors: Silva Isadora Ames , Só Gabriel Barcelos , Weissheimer Theodoro , Mendes Aline , Hashizume Lina Naomi , Só Marcus Vinícius Reis , Rosa Ricardo Abreu da 
Published: May 2022

Abstract
Objective
Evaluate the influence of ultrasonic activation (UA) on the physicochemical properties of calcium silicate sealers.
Methods
Nine experimental conditions were created based on the calcium silicate sealers (Bio-C Sealer, Sealer Plus BC, and Bio Root RCS) and the ultrasonic activation (no activation, 10 seconds, and 20 seconds). Activation was performed with an ultrasonic insert 20/.01. The mold for the physicochemical analysis was filled and evaluated according to the ANSI/ADA specification nº. 57: initial and final setting time, flow, radiopacity and solubility. Tests were also performed to evaluate pH and calcium ion release with experimental periods of 1, 24, 72, and 168 hours. Data were analyzed by one-way analysis of variance and post-hoc Tukey tests. The significance level was set at 5%.
Results
The time of UA progressively delayed the initial setting time for all calcium silicate sealers (P < .05). Twenty seconds of UA increased the mean flow values of Sealer Plus BC and Bio-C Sealer compared to NA (P < .05). UA did not influence the radiopacity and solubility of the tested sealers (P > .05). UA for 20 seconds enhanced the pH levels and the calcium ion release of Sealer Plus BC and Bio-C Sealer at 168h (P < .05).
Conclusion
UA for twenty seconds interferes with some physicochemical properties of calcium silicate sealers.
Clinical Relevance:
Twenty seconds of ultrasonic activation can improve the flowability, pH, and calcium ion release of Sealer Plus BC and Bio-C Sealer. Setting time was progressively delayed the longer the ultrasonic activation.
A real-time rural domestic garbage detection algorithm with an improved YOLOv5s network model
Authors: Jiang Xiangkui , Hu Haochang , Qin Yuemei , Hu Yihui , Ma Shuaiyin , Ding Rui 
Published: May 2022

Abstract
With the continuous promotion of green environmental protection and the circular economy, rural garbage classification and treatment have become a hot issue to be solved in China. A growing number of researchers use deep learning technology to classify and treat garbage in rural areas, and have achieved certain effect. Nevertheless, there are still the following issues that require to be optimized: (1) the cost of model training is high; (2) missing detection behavior when detecting small and multiple targets; (3) the precision and real-time of the model cannot be satisfied simultaneously. For the sake of solving these problems, we trained a garbage classification and detection model applied to rural areas based on the YOLOv5 algorithm. In general, there are four contributions in our work: (1) an attention combination mechanism is introduced to build a better backbone network structure; (2) a new small target detection layer is added to the head network in order to enhance the model's ability to detect small targets; (3) the CIoU loss function is used to optimize the output prediction bounding box; (4) the Adam optimization algorithm is selected to enhance the convergence speed of the model during the training, while reducing the degree of fluctuation of the loss value. Compared with the original YOLOv5s network model, the detection speed is improved by 10ms and the detection accuracy is improved by 4.6%. Compared with the mainstream detection algorithms Fast R-CNN, SSD and YOLOv3, the improved algorithm has higher FPS value and mAP@0.5 value, that is 47.6 frames/s and 96.4% respectively. Our proposed YOLOv5s-CSS model detects a single garbage image with the speed of 0.021s and the detection accuracy of 96.4%. Meanwhile it reduces the complexity of the network model to a certain extent, which can meet the requirements of real-time detection of rural domestic garbage.
Antioxidant and anti-inflammatory effects of DhHP-6 as a synthetic oxidase-mimickingmimicase enzyme in an experimental rat model of periodontitis
Authors: Yan Jiaqing , Zheng Lan , Zhu Ying , Chen Qiuyan , Yang Yimeng , Yu Huimei , Li Zhengqiang 
Published: May 2022

Abstract
Deuterohemin-ala-his-thr-val-glu-lys (DhHP-6) is a new type of synthetic oxidase-mimicking enzyme, has antioxidant effects and the ability to scavenge free radicals. It also has the potential to act as an antioxidant enzyme. In this study, an oxidative stress model of human gingival fibroblasts (HGFs) in vitro and a rat periodontitis model in vivo were established to study the antioxidant effect of DhHP-6 and its mechanism, the inflammatory response of periodontitis, and the protective effect of DhHP-6 on alveolar bone resorption induced by periodontitis. DhHP-6 reduced oxidative stress and the expression of inflammatory factors in HGFs. DhHP-6 had a dose-dependent protective effect on alveolar bone absorption in rats with periodontitis, enhanced rat antioxidant capacity and reduced inflammation. Micro-CT scanning showed that DhHP-6 reduced alveolar bone loss and improved the bone structure of the left maxillary first molar of rats. HE staining results showed that, compared with the control, there were no obvious morphological and histological changes in the rat organs. These results suggest that DhHP-6 can be used to treat experimental periodontitis in rats by increasing the expression levels of antioxidant enzymes and antioxidants in systemic and local tissues, which reduces the content of oxidation products and cyto-inflammatory factors.
Socio-economic and Socio-cultural Influences on Newly Qualified Physicians’ Preparedness to Practice Palliative Care in Nigeria - A Qualitative Study
Authors: Oji Nwabata , Onyeka Tonia , Soyannwo Olaitan , Paal Piret , Elsner Frank 
Published: May 2022

Abstract
Background
Dealing with life-limiting illnesses, death, dying and grief, is uncharted territory for the fresh medical graduate. It is a field that is heavily influenced by cultural, religio-spiritual and social factors. These add a complexity to palliative and end-of-life-care that challenges newly qualified physicians and requires the formation of appropriate knowledge, skills and attitudes in the junior doctor. This study aimed at obtaining insight into the perspectives and preparedness of newly qualified physicians in Nigeria, regarding the practice of palliative care.
Methods
The study was a cross-sectional, multicentre survey of newly qualified Nigerian physicians, using semi-structured qualitative interviews. The data was analysed, applying content-structuring qualitative content analysis.
Results
Forty semi-structured interviews were held with medical House Officers at two Nigerian tertiary institutions. The qualitative content analysis resulted in a comprehensive code system. Palliative care practice as experienced by young physicians in Nigeria was highlighted by four codes: Breaking Bad News; Pronouncing a Prognosis and Diagnosing Dying; Involving the Family and Pain and Symptom Management. Other codes highlighted possible influences on the newly qualified physicians’ palliative care practice. Main themes that were the major influencers here were: socio-economic considerations of a resource-limited setting and cultural-religious aspects characteristic to Nigeria.
Conclusions
This study offers valuable insight into potential socio-cultural and socio-economic variables on palliative care practice in Nigeria. Evidence on social, cultural and religio-spiritual dimensions of palliative care is indispensable for culturally sensitive care. The results could aid in the development of appropriate knowledge, skills and attitudes in newly qualified physicians through culturally and contextually appropriate palliative care training measures. The results may be applicable to other Sub-Saharan African settings and to African communities in the diaspora and may be used to improve future palliative care practice with a socio-cultural perspective.
Live-attenuated Japanese encephalitis virus inhibits glioblastoma growth and elicits potent antitumor immunity
Authors: Qi Zhongbing , Zhao Jing , Li Yuhua , Zhang Bin , Hu Shichuan , Chen Yanwei , Ma Jinhu , Shu Yongheng , Wang Yunmeng , Cheng Ping 
Published: May 2022

Abstract
Glioblastoma (GBM) is a highly aggressive form of brain tumor. GBM has become resistant to currently available conventional therapies, including surgery, radiation, and systemic chemotherapy. Here, we investigated the oncolytic properties of a live attenuated Japanese encephalitis vaccine strain (JEV-LAV) virus for the treatment of GBM. JEV-LAV demonstrated oncolytic activity against GBM tumor cells in vitro and inhibited its growth in vivo. Besides, it effectively prolonged the survival of tumor-bearing mice. Mechanistically, JEV-LAV increased the infiltration of CD8+ T cells into the tumor tissues and remodeled the immunosuppressive GBM microenvironment that is not conducive to immunotherapy. Consequently, when combined with an immune checkpoint inhibitor, JEV-LAV therapy improved the response of aPD-L1 blockade therapy against GBM. The safety of intracerebral injection of JEV-LAV in the treated animals further provided support for the clinical application of JEV-LAV as a GBM therapy.