bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Stirring Speed on Mechanical Properties of the Inorganic-Powder- Reinforced Epoxy Composite
Authors: Mao Lizhou , Yan Xuefeng , Yao Lu , Yu Hang , Cao Haijian , Shen Yue , Ma Yan 
Published: July 2022

Abstract
Inorganic powders (IPs), including SiO2, Al2O3, and Ca3Si3O9, were used as reinforcements and thermosetting epoxy resin was utilized as a matrix to manufacture IP/epoxy preform. The effects of stirring speed on the physical and mechanical properties of IP/epoxy composites were analyzed. Results showed that the tensile, flexural, and impact mechanical properties of IP/epoxy composites generally increased first and then decreased with the rise in stirring speed. They improved by 27.03%, 13.75%, and 8.78% when the stirring speed was 1000 r·min−1. When the stirring speed was less than 1000 r·min−1, the IP was unevenly distributed and prone to agglomeration and had weak junctions, which resulted in poor mechanical properties. When the stirring speed exceeded 1000 r·min−1, the particles with larger sizes were centrifuged, the powder was dispersed unevenly again, and the strength decreased.
Cardiotoxicity’s Differentially Expressed Genes of Tyrosine Kinase Inhibitor---Sorafenib Based on GEO Database
Authors: Yan Jingru , Li Yilan , Zhang Yao 
Published: July 2022

Abstract
Background.
Sorafenib exhibits cardiotoxicity while the mechanisms are not elucidated. It is of great necessity to further explore the molecular mechanism of cardiotoxicity of sorafenib.
Methods.
All original dataset and results of user are downloadable in GEO (GSE119122) database with the high throughput sequencing results of sorafenib cardiotoxicity’s the differentially expressed genes (DEGs) by Sprague Dawley rats in RNA levels. The raw data was used to find the DEGs by Gene Ontology (GO) consortium, Protein-Protein Interaction (PPI) network, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses and Gene Set Enrichment analyses (GSEA). Representative DEGs of mannose receptor C-type 1 (Mrc1) and Fc gamma receptor I a (Fcgr1a) was verified by the real-time PCR (RT-qPCR). The levels of ROS were measured using Reactive Oxygen Species Assay Kit and dihydroethidium (DHE). The Cell Counting Kit-8 (CCK-8) experiment revealed H9C2 cell viability. We can observe intracellular microstructure by transmission electron microscopy (TEM).
Results.
Following the advent of R language, 31 DEGs between sorafenib damage and control group were analyzed. The KEGG analyses showed that the differential genes were expressed in phagosome signaling pathway. The genes of Mrc1 and Fcgr1a were markedly enriched in the phagosome signaling pathway. The outcome of RT-qPCR demonstrated that the sorafenib damage leads to profound decline of genes of Mrc1 and Fcgr1a level. Results demonstrate that sorafenib significantly reduces cell viability tested by CCK-8. Sorafenib could cause an increase of ROS levels and the TEM show that mitochondria-endoplasmic reticulum contacts and so forth.
Conclusions.
The results confirm our hypothesis that the molecular mechanism of sorafenib cardiotoxicity is associated with phagosome. It might be a potential diagnosis and therapeutic target for sorafenib exhibited cardiotoxicity.
Neonatal Mortality in Edward Francis Small Teaching Hospitals (EFSTH) Banjul, The Gambia, 2021: a need for neonatal mortality surveillance?
Authors: Jallow Bubacarr , Adewuyi Adebayo Peter , Umeokonkwo Chukwuma David , Jallow Amadou Woury , Jatta Joseph W , Jallow Ebrima K , Bittaye Mustapha 
Published: July 2022

Abstract
Background: Neonatal mortality surveillance has not been prioritized in The Gambia, despite the global target of the SDGs to reduce Neonatal Mortality Rate to <12/1000 livebirth by 2030. We conducted this study to describe the epidemiology of neonatal mortality in Edward Francis Small Teaching Hospital in the Gambia January to December 2021.Methods: We conducted a cross sectional study in (EFSTH) among neonates who died within the period under study. We extracted data from various sources in the hospital. We analyzed demographic variables, listed sources of data, and determined risk factors for death at 95% confidence interval. Results: Of the 306 neonatal deaths in 2021, 161 (52.6%) were female, the median age is 2.4 days at a range of 0.04-26 days. Majority 242 (79.1%) of the neonates that died in EFSTH were delivered via vagina spontaneously. There were more 174 (56.9) preterm and 95 (35.3%) very low birth weight babies. The highest cause of death was pneumonia among 82 (26.8%), while highest non-infectious cause of death was asphyxia accounting for 29 (9.5%) deaths. Male neonates and neonates born in Western regions (Western1 & 2) are more likely to die of infectious causes compared to those from other 5 regions [POR:1.36 (CI= 0.8513-2.1679)] and [POR 0.57 (CI= 0.2650-1.2120)] respectively. These possible risk factors are not statistically significant. Sources of data varies for each hospital: case folders, admission registers, hospital database and death certificates. Conclusion: We found that the neonatal mortality rate for the hospital is higher than the national one. More female neonates die and majority of the mortalities occur within the first day of life. More deaths occur in the Western Regions who are less likely to die of infectious causes as compared to other regions. The leading causes of deaths among neonates were found to be pneumonia, sepsis, low birth weights among others.
Correlation between thyroid function, sensitivity to thyroid hormones and metabolic dysfunction-associated fatty liver disease in euthyroid subjects with newly diagnosed type 2 diabetes
Authors: Zhang Xiaodan , Chen Yimei , Ye Huiyu , Luo Zirui , Li Jiahong , Chen Zhishan , Zhang Enting , Li Wangen 
Published: July 2022

Abstract
Purpose
To estimate the prevalence of metabolic dysfunction-associated fatty liver disease (MAFLD) and to evaluate the associations between thyroid parameters, MAFLD and liver fibrosis in euthyroid patients with newly diagnosed type 2 diabetes mellitus (T2DM).
Methods
Overall, 689 patients with newly diagnosed T2DM and 110 subjects without diabetes were included. All the participants were euthyroid, and were categorized as non-MAFLD and MAFLD. Demographic information, biochemical parameters, and serum thyroid hormones were collected. The thyroid hormone sensitivity indices were calculated. MAFLD was defined according to abdominal ultrasound and clinical manifestations. Noninvasive fibrosis indices were calculated to identify advanced liver fibrosis.
Results
The prevalence of MAFLD was significantly higher in patients with T2DM than in subjects without diabetes. Levels of free triiodothyronine (FT3) and FT3 to free thyroxine (FT4) ratio were significantly higher in subjects with MAFLD. In patients with T2DM, levels of thyroid stimulating hormone (TSH), Thyroid feedback quantile-based index (TFQIFT3) calculated using FT3 and TSH, thyrotroph T3 resistance index (TT3RI) and thyrotroph T4 resistance index (TT4RI) were significantly higher in subjects with MAFLD. The prevalence of MAFLD increased with the rise of FT3, FT3/FT4, TSH, and sensitivity to thyroid hormone indices (TFQIFT3, TT3RI, and TT4RI). Positive correlations were significant between FT3, TFQIFT3 and MAFLD. But after further adjusted for BMI and HOMA-IR, the correlations were not significant. The incidence of advanced fibrosis tended to increase as the rise of TSH and sensitivity to thyroid hormone indices (TFQIFT3, TT3RI, and TT4RI).
Conclusion
MAFLD was prevalent in euthyroid patients with newly diagnosed T2DM. Higher normal FT3, TSH and impaired sensitivity to thyroid hormones are associated with increased risk of MAFLD.
Adaptive coexistence of synchronization and anti-synchronization for a class of switched chaotic systems
Authors: Ren Ling 
Published: July 2022

Abstract
This paper addresses the problem of coexistence of synchronization and anti-synchronization (CSAS) for a class of switched chaotic systems by adaptive control method. The unified chaotic system as the subsystems of the switched system is investigated. Firstly, two theorems describe the necessary and sufficient condition for the CSAS of the chaotic systems and proofed rigorously. Secondly, according to the given two theorems, two algorithms are given to search the synchronization variables and anti-synchronization variables in the chaotic systems. Thirdly, the CSAS of the switched chaotic system can be achieved by the designed adaptive global controller with only one input channel under the arbitrary switching signal. Finally, the numerical simulation results verify the validity and effectiveness of the method we obtained.
Improved multi-trait prediction of wheat end-product quality traits by integrating NIR predictions
Authors: Azizinia Shiva , Mullan Daniel , Rattey Allan , Godoy Jayfred , Robinson Hannah , Moody David , Forrest Kerrie , Keeble-Gagnere Gabriel , Hayden Matthew J. , Tibbits Josquin FG , Daetwyler Hans D 
Published: July 2022

Abstract
Historically, end-product quality testing has been costly and required large flour samples and therefore, was generally implemented in late phases of variety development imposing huge cost on the breeding effort and effectiveness. High genetic correlations of end-product quality traits with higher-throughput and non-destructive testing technologies, such as near infra-red (NIR), could enable early-stage testing and effective selection on these highly valuable traits in a multi-trait genomic prediction model. We studied the impact on end-product quality trait accuracy of adding NIR phenotypes as a second trait in genomic best linear unbiased prediction (GBLUP) to increase training population size of six quality traits (crumb yellowness, water absorption, texture hardness, flour yield, grain protein, flour swelling volume). 1400-1900 bread wheat lines were measured during 2012-2019 in laboratory assays along with NIR records of approximately 27,000 lines. All lines were genotyped with the Infinium™ Wheat Barley 40K BeadChip and imputed using exome sequence data. End-product and NIR phenotypes were genetically correlated (0.5–0.83, except for Flour Swelling Volume 0.19). Prediction accuracies of end-product traits ranged between 0.28–0.64 and increased by 30% through inclusion of NIR predicted phenotypes compared to single trait analysis. There was a high correlation between the magnitude of multi-trait prediction accuracy and genetic correlations between end-product and NIR phenotypes (0.69–0.77). Our forward prediction validation revealed a gradual increase in prediction accuracy when adding more years to the multi-trait model. Overall, we achieved genomic prediction accuracies at a level that enables selection for end-product quality traits early in the breeding cycle.
Base editing derived models of human WDR34 and WDR60 disease alleles replicate retrograde IFT and hedgehog signaling defects and suggest disturbed Golgi protein transport
Authors: Antony Dinu , Güleç Elif Yýlmaz , Bakey Zeineb , Schüle Isabel , Kim Gwang-Jin , Skatulla Ilona , Cathomen Toni , Brunner Han , Arnold Sebastian , Schmidts Miriam 
Published: July 2022

Abstract
Cytoplasmic Dynein-2 / IFT-dynein is the only known retrograde motor for intraflagellar transport. Dysfunction of WDR34 and WDR60, the two intermediate chains of this complex, causes Short Rib Thoracic Dystrophy (SRTD), human skeletal chondrodysplasias with high lethality. Complete loss of function of WDR34 or WDR60 is lethal in vertebrates and individuals with SRTD carry at least one putative hypomorphic missense allele. Gene knockout is therefore not suitable to study the effect of these human missense disease alleles. Using CRISPR single base editors, we recreated three different patient missense alleles.Consistent with previous findings in the dynein-2 full loss of function models and patient fibroblasts, mutant cell lines showed hedgehog signaling defects as well as disturbed retrograde IFT. Transcriptomics analyses revealed differentially regulated expression of genes associated with various biological processes, including regulation of the actin cytoskeleton. Further, we observed differential regulation of genes associated with Golgi intracellular transport. In addition to providing cellular model systems enabling investigations of the effect of human SRTD disease alleles, our findings indicate non-ciliary functions for WDR34 and WDR60 in addition to the established roles as components of the retrograde IFT motor complex in cilia.
CT-maximal Diameters and mean CT value of Synchronous Multiple Lung Tumors Adenocarcinoma Are Smaller Than Solitary Lung Tumors In Pure Ground-glass Opacities
Authors: Wang Longfei , Zhao Lei , Zhao Weijun , Li Xinjian , Xu Qingtao , Liang Zhigang 
Published: July 2022

Abstract
Background: The features of multiple GGOs, especially for synchronous multiple pure GGOs (SMpGGOs), remains unknown. This retrospective study aimed to investigate the clinical and radiological features of SMpGGOs. Methods: Patients with solitary pure GGOs (SpGGOs) or SMpGGOs at Ningbo First Hospital from August 2018 to June 2020 were included. The clinical records and radiological image features between SpGGOs and SMpGGOs were compared. Data were analyzed using SPSS 21.0. Results: 138 patients (58 patients with 58 SpGGOs, 80 patients with 187 SMpGGOs) were evaluated. According to the multivariate analyses, maximal diameter (p＜0.001) and mean CT value (p=0.003) were independent risk factors. Receiver operating curves revealed that the optimal threshold of maximal diameters was 12.5 mm for SpGGOs (sensitivity 54.5%, specificity 100%, AUC 0.851) and 5.5 mm for SMpGGOs (sensitivity 86.4%, specificity 55.6%, AUC 0.777). The optimal threshold of mean CT value was -531.9 Hu for SpGGOs (sensitivity 43.2%, specificity 100%, AUC 0.724) and -615.0 Hu for SMpGGOs (sensitivity 61.4%, specificity 66.7%, AUC 0.651). Conclusion: The maximal diameters and mean CT value were significant independent predictors for adenocarcinoma of pGGOs. In SMpGGOs, the optimal threshold of maximal diameters and mean CT value of adenocarcinoma were smaller than those of SpGGOs.
Association between fetal sex and metabolic syndrome in women 40 and older: the REACTION study
Authors: Xie Qian , Li Ruoqing , Wan Qin , Tong Nanwei 
Published: July 2022

Abstract
Aims
The purpose of this study was to determine if fetal sex has an effect on risk of metabolic syndrome (MetS) in women in China.
Methods
A cohort of 3758 singleton pregnant Chinese women, aged 40 years or older were included. For each of these women, registry data on all pregnancies and miscarriages were obtained. This was a retrospective cohort study that aimed to determine whether fetal sex was associated with metabolic syndrome. We defined MetS as meeting five criteria, namely ineffective insulin metabolism, reduced glucose tolerance, high blood pressure, dyslipidemia and large waist size.
Results
Among 3758 singleton pregnant women 40 and older in Luzhou city, there were 1018 (27.1%) participants with incident MetS. Mothers carrying a male fetus(male group) had higher triglyceride(TG) and total cholesterol(TC) and showed greater diabetes background than the women carrying a female fetus (female group) (P < 0.05). MetS occurred more often with a male than female fetus but the difference was not statistically significant(P > 0.05). MetS frequency was statistically more in the male group than female group in postmenopausal women (P < 0.05). MetS occurred significantly less frequently in premenopausal compared to postmenopausal women in both the male and female group (P < 0.05). MetS occurred more often in the male group in postmenopausal women, showing statistical significance (P < 0.05).
Conclusions
Our study found that fetal sex was not associated with MetS in Chinese women 40 and older. But in postmenopausal women, MetS was seen statistically more often in the male group. We should pay more attention to postmenopausal women who have a male fetus and take early measures to prevent related chronic diseases.
Investigating potential gender-based differential item functioning for items in the Kansas City Cardiomyopathy Questionnaire (KCCQ) Physical Limitations domain
Authors: Coles Theresa M. , Lin Li , Weinfurt Kevin , Reeve Bryce B. , Spertus John A. , Mentz Robert J. , Piña Ileana L. , Bocell Fraser D. , Tarver Michelle E. , Saha Anindita , Caldwell Brittany 
Published: July 2022

Abstract
Background: Women with heart failure report worse health-related quality of life, on average, than men. Whether this is due to true differences in care or that women and men interpret and respond to survey questions differently is unknown. In this study, we investigate potential gender-based differential item functioning (DIF) on the Kansas City Cardiomyopathy Questionnaire (KCCQ) Physical Limitations (PL) domain.Methods: Using data from Heart Failure: A Controlled Trial Investigating Outcomes of Exercise Training (HF-ACTION), a multicenter, randomized controlled trial of exercise training in patients with chronic heart failure with reduced ejection fraction (1670 men, 661 women), we assessed gender DIF using item response theory (IRT)–based Wald test and ordinal logistic regression. Both methods evaluated how men and women responded to each KCCQ item after adjusting for their physical limitation status.Results: A 5-item version of the KCCQ PL domain was evaluated to satisfy statistical assumptions of DIF methodology. No KCCQ items exhibited statistically significant DIF using the IRT-based Wald method, and two items exhibited DIF using the ordinal logistic regression method (KCCQ1e: Climbing a flight of stairs without stopping; KCCQ1f: Hurrying or jogging) (p<0.01), but the magnitude of DIF was negligible. Conclusions: To accurately measure patient-reported outcomes, it is important to evaluate potential biases that may influence the ability to compare patient subgroups. The magnitude of DIF on the 5-item KCCQ PL domain was negligible.
Skilled aerobic exercise reduces endoplasmic reticulum stress and dopamine neuron apoptosis by activating Wnt/β-catenin pathway and improves motor function in PD model rats
Authors: Ruisi TIAN , Zhengquan ZHU , Hua GAO , LI Peishan , YANG Xinling , LI Yan-xia 
Published: July 2022

Abstract
Objective
To observe whether skilled aerobic exercise (SAE) could reduce endoplasmic reticulum stress(ERS) and apoptosis of dopamine neuron by activating Wnt/β-catenin pathway and improves motor function in Parkinson's disease(PD) model rats.
Methods
The PD models were established by injection into the right SNC(substantia nigra,compact part) and VTA(ventral tegmental area) on the rat stereolocator with 6-hydroxydopamine(6-OHDA). A cylindrical test was used to was used to test motor function. The expression of tyrosine hydroxylase(TH) in midbrain was detected by immunohistochemistry, the expression of genes and proteins related to the Wnt/β-catenin pathway was detected by Western blot. The level of Irisin in serum was identified through an enzyme-linked immunosorbent assay (ELISA).
Results
It was determined that 28 days of SAE improved motor function from Parkinson disease. In model group, the expression of TH in midbrain and FNDC5 in skeletal muscle was significantly down-regulated, the content of Irisin in serum decreased, the expression level of Bip/Grp78 and CHOP was up-regulated, the expression level of Wnt, β-catenin and P-GSK3β (Ser9) were down-regulated, while P-GSK3β (Tyr216) was up-regulated, the movement ability of forelimbs decreased (P < 0.05), and the rotation behavior induced by Apo significantly increased in model group (P < 0.05).
Conclusions
Mid-long term SAE could up-regulate the expression of FNDC5 in skeletal muscle, increase the content of Irisin in serum, reduce the level of ERS in midbrain, increase the activity of Wnt/β-catenin pathway, down-regulate the activity of Gsk3β, reduce the apoptosis of DA neurons in midbrain substantia nigra, and improve the motor function of PD model rats.
A Computer Tomography Radiomics-based model to Predict Survival of Patients with EGFR‑Mutated Non‑small‑cell Lung Cancer
Authors: Huang Chen , Cao Yubin , Zhu Lei , Xiong Qunli , Zhang Qin 
Published: July 2022

Abstract
Background
To evaluate the prognosis of EGFR-mutated non-small-cell lung cancer(NSCLC) patients and accurately target the patient subgroups with best potential outcome greatest, a simpler and more effective model based on readily available laboratory parameters is needed in clinical practice.
Methods
Totally, computed tomographic (CT) images from 162 EGFR-mutated NSCLC patients at the time of first diagnosis in West China Hospital of Sichuan University were retrospectively collected and analysed to describe the target area of the lesion. All patients were randomly divided into a training cohort and a validation cohort. Radiomic features from images were extracted. The least absolute shrinkage selection operator (LASSO) regression method was used to screen valuable radiomic features. The logistic regression method was used to establish a radiomic model, and the nomogram was used to evaluate the discrimination ability. Receiver characteristic curve (ROC) and decision curve analysis (DCA) were used to evaluate the performance of the model.
Results
We established a nomogram that combined 6 important clinical factors and imaging risk scores to predict the three-year and five-year survival rates of EGFR-mutated NSCLC patients. The AUC-ROC of three-year OS was 0.715, and the five-year OS was 0.705, which indicated a favourable discrimination capability of our nomogram and its great potential in targeting clinical services and predicting patient outcomes.
Conclusion
Based on chest CT imaging and clinicopathological features, including age, sex, Stage_T, Stage_N, Stage_M, clinical stage, and imaging risk score, we constructed and validated a nomogram for predicting an individualized prediction of survival for patients with EGFR‑mutated NSCLC.
The ceRNA network regulates epithelial–mesenchymal transition in colorectal cancer
Authors: Li Ruina , Gao Xiaoling 
Published: July 2022

Abstract

Epithelial–mesenchymal transition (EMT), a biological process transformed by a specific program, is involved in the progression and metastasis of colorectal cancer (CRC). Recently, emerging evidence showed that dysregulation of non-coding RNA (ncRNA) was related to EMT. The ncRNAs, including long non-coding RNA (lncRNA), regulate the transcription of downstream target genes (messenger RNA [mRNA]) through interaction with microRNAs (miRNAs); these are termed competitive endogenous RNA (ceRNA) networks. ceRNA dysregulation-induced EMT, which is closely related to the progression and prognosis of CRC, has attracted the attention of researchers. However, the understanding of ceRNA network regulation in CRC remains limited.We discussed the molecular functions of lncRNA, the ceRNA networks related to miRNAs and mRNAs in EMT, and EMT transcription factors zinc finger E-box binding homeobox 1/2 (ZEB1/2), SNAIL, SLUG, and TWIST1/2 regulated by the ceRNA network axis. In addition, the miRNAs and lncRNAs which directly target genes, thereby initiating different signaling pathways to promote EMT in CRC, were summarized. Clarification of the role of these molecules in the EMT is key for the diagnosis of CRC.