bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mre11 liberates cGAS from nucleosome sequestration during tumorigenesis
Authors: Cho Min-Guk , Kumar Rashmi J. , Lin Chien-Chu , Boyer Joshua A. , Shahir Jamshaid A. , Fagan-Solis Katerina , Simpson Dennis A. , Fan Cheng , Foster Christine E. , Goddard Anna M. , Wang Qinhong , Wang Ying , Ho Alice Y. , Liu Pengda , Perou Charles M. , Zhang Qi , McGinty Robert K. , Purvis Jeremy E. , Gupta Gaorav P. 
Published: December 2022

AbstractOncogene-induced replication stress generates endogenous DNA damage that activates cGAS/STING-mediated innate immune signaling and tumor suppression1-3. However, the mechanism for cGAS activation by endogenous DNA damage remains enigmatic, particularly given the constitutive inhibition of cGAS by high-affinity histone acidic patch (AP) binding4-10. Here we report anin vivoCRISPR screen that identified the DNA double strand break sensor Mre11 as a suppressor of mammary tumorigenesis induced by Myc overexpression and p53 deficiency. Mre11 antagonizes Myc-induced proliferation through cGAS/STING activation. Direct binding of the Mre11-Rad50-Nbn (MRN) complex to nucleosomes displaces cGAS from AP sequestration, which is required for DNA damage-induced cGAS mobilization and activation by cytosolic DNA. Mre11 is thereby essential for cGAS activation in response to oncogenic stress, cytosolic DNA transfection, and ionizing radiation. Furthermore, we show Mre11-dependent cGAS activation suppresses Myc-induced proliferation through ZBP1/RIPK3/MLKL-mediated necroptosis. In human triple-negative breast cancer, ZBP1 downregulation correlates with increased genome instability, decreased immune infiltration, and poor patient prognosis. These findings establish Mre11 as a critical link between DNA damage and cGAS activation that regulates tumorigenesis through ZBP1-dependent necroptosis.One-sentence summaryMre11 is required for cGAS activation during oncogenic stress and promotes ZBP1-dependent necroptosis.
Tryptophan-like side chain holding aptamers inhibit respiratory syncytial virus infection of lung epithelial cells
Authors: Percze Krisztina , Tolnai Zoltán János , Eleveld Marc , Ou Li , Du Haijuan , Olia Adam S. , Kwong Peter D. , de Jonge Marien I. , Mészáros Tamás 
Published: December 2022

ABSTRACTRespiratory syncytial virus (RSV) is a leading cause of serious and even fatal acute lower respiratory tract infections in infants and in the elderly. Potent RSV neutralization has been achieved by antibodies that selectively bind the prefusion form of the viral fusion (F) protein. We hypothesised that similar potent neutralization could be achieved using F protein targeting aptamers. Aptamers have yet to reach their translational potential for therapeutics or diagnostics due to their short half-life and limited range of target-aptamer interactions; these shortcomings can, however, be ameliorated by application of amino acid-like side chain holding nucleotides. In this study, a stabilized version of the prefusion RSV F protein was targeted by aptamer selection using an oligonucleotide library holding a tryptophan-like side chain. This process resulted in aptamers that bound the F protein with high affinity and differentiated between pre- and postfusion conformation. Identified aptamers inhibited viral infection of lung epithelial cells. Moreover, introduction of modified nucleotides extended aptamer half-lives. Our results suggest that targeting aptamers to the surface of viruses could yield effective drug candidates, which could keep up with the pace of the continuously evolving pathogens.
p38γ and p38δ modulate innate immune response by regulating MEF2D activation
Authors: Escós Alejandra , Díaz-Mora Ester , Pattison Michael , Fajardo Pilar , González-Romero Diego , Risco Ana , Martín-Gómez José , Bonneil Éric , Sonenberg Nahum , Jafarnejad Seyed Mehdi , Sanz-Ezquerro Juan José , Ley Steven C. , Cuenda Ana 
Published: December 2022

AbstractEvidence implicating p38γ and p38δ (p38γ/p38δ) in inflammation are mainly based on experiments using p38γ/p38δ deficient (p38γ/δ-/-) mice, which show low levels of TPL2, the kinase upstream of MKK1-ERK1/2 in myeloid cells. This could obscure p38γ/p38δ roles, since TPL2 is essential for regulating inflammation. Here we generated a p38γD171A/D171A/p38δ-/-(p38γ/δKIKO) mouse, expressing kinase-inactive p38γ and lacking p38δ. This mouse exhibited normal TPL2 levels, making it an excellent tool to elucidate specific p38γ/p38δ functions. p38γ/δKIKO mice showed a reduced inflammatory response and less susceptibility to LPS-induced septic shock andCandida albicansinfection than wild-type mice. Gene expression analyses in LPS-activated WT and p38γ/δKIKO macrophages revealed that p38γ/p38δ regulated numerous genes implicated in innate immune response. Additionally, phospho-proteomic analyses andin vitrokinase assays showed that the transcription factor myocyte enhancer factor-2D (MEF2D) was phosphorylated at Ser444 via p38γ/p38δ. Mutation of MEF2D Ser444 to the non-phosphorylatable residue Ala increased its transcriptional activity and the expression ofiNOSandIL-1βmRNA. These results suggest that p38γ/p38δ govern innate immune responses by regulating MEF2D phosphorylation and transcriptional activity.
Antimicrobial susceptibility testing for diverseMalasseziaspecies
Authors: Rathie Brooke , Theelen Bart , Laurence Martin , Shapiro Rebecca S. 
Published: December 2022

AbstractThe genusMalasseziais an opportunistic lipid-dependent yeast that is associated with common skin diseases and has recently been associated with Crohn’s disease and certain cancers. Understanding the susceptibility ofMalasseziato diverse antimicrobial agents is crucial for identifying effective antifungal therapies. Here, we tested the efficacy of isavuconazole, itraconazole, terbinafine and artemisinin against threeMalasseziaspecies:M. restricta, M. slooffiae, andM. sympodialis, using microbroth dilution techniques. We found antifungal properties for the two previously-unstudied antimicrobials: isavuconazole and artemisinin. Overall allMalasseziaspecies were particularly susceptible to itraconazole, with a MIC range from 0.0015 to 0.1562 μM.ImportanceTheMalasseziagenus is known to be a cause of skin conditions and has recently been associated with diseases such as Crohn’s disease, pancreatic ductal carcinoma and breast cancer. This work was completed to assess the susceptibility to a variety of antimicrobial drugs on diverseMalasseziaspecies, in particularMalassezia restricta.
Frequent first-trimester pregnancy loss in rhesus macaques infected with African-lineage Zika virus
Authors: Rosinski Jenna R. , Raasch Lauren E. , Tiburcio Patrick Barros , Breitbach Meghan E. , Shepherd Phoenix M. , Yamamoto Keisuke , Razo Elaina , Krabbe Nicholas P. , Bliss Mason I. , Richardson Alexander D. , Einwalter Morgan A. , Weiler Andrea M. , Sneed Emily L. , Fuchs Kerri B. , Zeng Xiankun , Noguchi Kevin K. , Morgan Terry K. , Alberts Alexandra J. , Antony Kathleen M. , Kabakov Sabrina , Ausderau Karla K. , Bohm Ellie K. , Pritchard Julia C. , Spanton Rachel V. , Ver Hoove James N. , Kim Charlene B. Y. , Nork T. Michael , Katz Alex W. , Rasmussen Carol A. , Hartman Amy , Mejia Andres , Basu Puja , Simmons Heather A. , Eickhoff Jens C. , Friedrich Thomas C. , Aliota Matthew T. , Mohr Emma L. , Dudley Dawn M. , O’Connor David H. , Newman Christina M. 
Published: December 2022

AbstractIn the 2016 Zika virus (ZIKV) pandemic, a previously unrecognized risk of birth defects surfaced in babies whose mothers were infected with Asian-lineage ZIKV during pregnancy. Less is known about the impacts of gestational African-lineage ZIKV infections. Given high human immunodeficiency virus (HIV) burdens in regions where African-lineage ZIKV circulates, we evaluated whether pregnant rhesus macaques infected with simian immunodeficiency virus (SIV) have a higher risk of African-lineage ZIKV-associated birth defects. Remarkably, in both SIV+ and SIV-animals, ZIKV infection early in the first trimester caused a high incidence (78%) of spontaneous pregnancy loss within 20 days. These findings suggest a significant risk for early pregnancy loss associated with African-lineage ZIKV infection and provide the first consistent ZIKV-associated phenotype in macaques for testing medical countermeasures.
Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in theDrosophilapupal wing
Authors: Piscitello-Gómez Romina , Gruber Franz S , Krishna Abhijeet , Duclut Charlie , Modes Carl D , Popović Marko , Jülicher Frank , Dye Natalie A , Eaton Suzanne 
Published: December 2022

AbstractHow morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing ofDrosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. We showed previously (Etournay et al., 2015) that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned. The core planar cell polarity (PCP) pathway influences morphogenetic cell movements in many other contexts, which suggests that it could globally pattern active cellular behaviors during pupal wing morphogenesis. We show here, however, that this is not the case: there is no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation, they do not affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes duringDrosophilapupal wing morphogenesis are independent of one potential chemical guiding cue, core PCP.
Primary Cilia are WNT Transducing Organelles whose Biogenesis is Regulated by a WNT┫ PP1 axis
Authors: Zhang Kaiqing , Da Silva Fabio , Seidl Carina , Wilsch-Bräuninger Michaela , Herbst Jessica , Huttner Wieland B. , Niehrs Christof 
Published: December 2022

SUMMARYWNT signalling is of paramount importance in development, stem cell maintenance, and disease. WNT ligands typically signal via receptor activation at the plasma membrane to induce β-catenin-dependent gene activation. Here we show that in primary cilia, WNT receptors relay a WNT/GSK3 signal that β-catenin-independently promotes ciliogenesis. Innovations supporting this conclusion are monitoring acute WNT co-receptor activation (phospho-LRP6) and identifying and mutating the LRP6 ciliary targeting sequence. Ciliary WNT signalling inhibits protein phosphatase 1 (PP1) activity, a negative regulator of ciliogenesis, by decommissioning GSK3-mediated phosphorylation of the PP1 regulatory inhibitor subunit PPP1R2. Accordingly, deficiency of WNT/GSK3 signalling by depletion of cyclin Y and cyclin-Y-like protein 1 induces widespread primary cilia defects in mouse embryonic neuronal precursors, kidney proximal tubules, and adult mice preadipocytes. We conclude that primary cilia are WNT ┫ PP1 signalling organelles.Graphical AbstractA Localized WNT ┫ PP1 Signalling Axis Promotes CiliogenesisThe WNT co-receptor LRP6 localizes to the ciliary membrane, where it is phospho-primed via a CCNY/L1-dependent CDK (not shown). WNT signalling inhibits GSK3 (not shown) and leads to inhibition of Protein phosphatase 1, a negative regulator of ciliogenesis. Right, CCNY/L1 deficiency disrupts the WNT ┫ PP1 signalling axis, leading to ciliary defects.
Exploring consumers' motives for electric vehicle adoption: bridging the attitude–behavior gap
Authors: Chaturvedi Pallavi , Kulshreshtha Kushagra , Tripathi Vikas , Agnihotri Durgesh 
Published: December 2022

PurposeThe current study aims to investigate the various consumption motives (hedonic, gain and normative) responsible for strengthening consumers' intentions toward purchase behavior for electric vehicle (EV).Design/methodology/approachA total of 411 valid survey responses were collected using a structured questionnaire. Data were analyzed using confirmatory factor analysis and structural equation modeling to investigate the empirical fit of the hypothesized framework.FindingsThe results of structural equation modeling revealed that all three motives were positively correlated with purchase intentions for EV. Hedonic motives were found to have the strongest influence on purchase intentions. In addition, gain and normative motives were also found to be significant predictors of EV buying behavior. Further analysis revealed a positive correlation between gain, normative and hedonic motives. Moreover, personal moral standards seem to have a significant and positive impact on the positive emotions associated with buying EV.Practical implicationsThe results of current research can be useful for marketers while designing promotional strategies for all the high-involvement green products. Marketing professionals and policymakers can use these results to build effective marketing strategies for EVs and reduce greenhouse gas emissions resulting from personal vehicle use.Originality/valueTo the best of the authors' knowledge, this is the first study in the South Asian region that explores consumers' motives for EV purchase behavior. Further, this is among a few studies, which have attempted to investigate the impact of hedonic, gain and normative motives on green purchase behavior in the context of high involvement green products.
Pay transparency, job turnover intentions and the mediating role of perceived organizational support and organizational justice
Authors: Stofberg Rosanna , Bussin Mark , Mabaso Calvin M. 
Published: December 2022

PurposeDespite widespread media attention and growing interest from researchers, pay transparency remains an under-studied field of research and its impact on organizational outcomes like job turnover is not well understood. This study explores the impact of pay transparency on job turnover intentions through the mediating effect of perceived organizational support (POS) and organizational justice.Design/methodology/approachData from quantitative surveys conducted with 299 employees at four South African organizations with different pay transparency practices were used to test the conceptual model of pay transparency impacting job turnover intentions through the mediators of POS and organizational justice.FindingsThe authors found a weak negative relationship between pay transparency and job turnover intentions and the role of the mediating variables was confirmed. Unexpectedly, the role of the organization emerged as a key variable. Controlling for organization type showed that the direct effect of pay transparency on turnover intentions became insignificant, indicating a stronger effect from organizational factors, of which pay transparency practices are just one.Originality/valueIdentifying a contextual (organizational) dimension to pay transparency practices extends the understanding of this concept and has implications for practice. The study also makes a methodological contribution by demonstrating the value of linking respondent data to a particular organization when researching pay transparency.
Local heat flux estimation inside tubes through conjugate gradient method with adjoint operator: application to the pulsating heat pipes case
Authors: Colaço Marcelo , Bozzoli Fabio , Cattani Luca , Pagliarini Luca 
Published: December 2022

Purpose
The purpose of this paper is to apply the conjugate gradient (CG) method, together with the adjoint operator (AO) to the pulsating heat pipe problem, including some quite interesting experimental results. The CG method, together with the AO, was able to estimate the unknown functions more efficiently than the other techniques presented in this paper. The estimation of local heat transfer coefficients, rather than the global ones, in pulsating heat pipes is a relatively new subject and presenting a robust, efficient and self-regularized inverse tool to estimate it, supported also by some experimental results, is the main purpose of this paper. To also increase the visibility and the general use of the paper to the heat transfer community, the authors include, as supplemental material, all numerical and experimental data used in this paper.

Design/methodology/approach
The approach was established on the solution of the inverse heat conduction problem in the wall by using as starting data the temperature measurements on the outer surface. The procedure is based on the CG method with AO. The here proposed approach was first verified adopting synthetic data and then it was validated with real cases regarding pulsating heat pipes.

Findings
An original fast methodology to estimate local convective heat flux is proposed. The procedure has been validated both numerically and experimentally. The procedure has been compared to other classical methods presenting some peculiar benefits.

Practical implications
The approach is suitable for pulsating heat pipes performance evaluation because these devices present a local heat flux distribution characterized by an important variation both in time and in space as a result of the complex flow patterns that are generated in this type of devices.

Originality/value
The procedure here proposed shows these benefits: it affords a general model of the heat conduction problem that is effortlessly customized for the particular case, it can be applied also to large datasets and it presents reduced computational expense.
Exploring mobile banking adoption and service quality features through user-generated content: the application of a topic modeling approach to Google Play Store reviews
Authors: Çallı Levent 
Published: December 2022

PurposeThe primary purpose of this research is to analyze the online user reviews, where real customer experiences can be observed, with text mining and machine learning approaches, which are seen as a gap in the related literature. This study aims to compare the latent themes uncovered by the topic modeling approach with studies focused on both mobile banking (m-banking) adaptation and service quality features, suggest new aspects and examine the effect of latent topics on customer satisfaction.Design/methodology/approachThis study analyzed 21,526 reviews posted by customers of private and state banks operating in Türkiye. An unsupervised machine learning method, Latent Dirichlet algorithm (LDA), was conducted to reveal topics, and the distribution of all reviews was visualized with the t-SNE algorithm. Random Forest, logistic regression, k-nearest neighbors (kNN) and Naive Bayes algorithms were utilized to predict user satisfaction through the given score.FindingsIn total, 11 topics were revealed by considering user reviews based on their experience. Among these topics, perceived usefulness and convenience and time-saving are much more important in the scoring given to m-banking apps. Furthermore, in more detail, seven topics have been identified related to technical and security problems related to m-banking apps.Originality/valueThis paper is a pioneer study regarding the method used and sample size reached in the m-banking literature. The findings also provide fresh insight into the post-Covid-19 era, both academically and practically, by providing new features for mobile bank adoption.
Competition or collaboration for human–robot relationship: a critical reflection on future cobotics in hospitality
Authors: Khoa Do The , Gip Huy Quoc , Guchait Priyanko , Wang Chen-Ya 
Published: December 2022

Purpose
The hospitality industry has recently witnessed explosive growth in robotization with the replacement of robots in many areas. Yet, a key consideration in this robotics wave is whether competition (i.e. robots take over all human tasks) or collaboration (i.e. humans collaborate closely with robots to perform work better) will define the future of the hospitality workspace. The purpose of this paper is to shed light on this controversial issue by taking a collaborative perspective to address the future human–robot relationship in hospitality workplace (i.e. cobotic team).

Design/methodology/approach
Drawing upon relevant theories and extant robotics literature, this paper will develop a critical reflection on the management of future cobotic team as a new phenomenon in hospitality industry.

Findings
The successful management of cobotics in hospitality lies in three interrelated key domains: feeling intelligence training for frontline employees, ethics governance for cobotics and trust building toward robot partners.

Practical implications
How to manage this cobotic team efficiently will be a focus for hospitality managers in the coming years. This paper offers several managerial insights for hospitality managers and practitioners regarding effectively managing the future collaboration between humans and robots within a dynamic work environment.

Originality/value
This study addresses cobotics as a critical yet unaddressed shift in the contemporary hospitality sector and proposes a framework highlighting three key domains for managing this cobotic team effectively. This framework also sets the direction to encourage more future empirical research exploring cobotic workforce in hospitality.
Public investment efficiency in WAEMU zone: do financing sources matter?
Authors: Bamba Moulaye , Somé Juste 
Published: December 2022

Purpose
This paper aims to assess the efficiency of public investment in West African Economic and Monetary Union (WAEMU) countries at both the global and sectoral level over the 2005–2015 period.

Design/methodology/approach
This paper estimates efficiency scores using stochastic frontier analysis (SFA) models. Efficiency is divided into managerial efficiency (related to inputs management) and technological efficiency (related to production technology). A Tobit model is then used to investigate the determinants of public investment efficiency.

Findings
The findings suggest that, at the global level, WAEMU countries are less efficient than sub-Saharan African and Asian reference countries. However, the breakdown of global efficiency into managerial and technological reveals that WAEMU countries are more efficient than sub-Saharan African countries in terms of technological efficiency. Moreover, these findings are robust to nonparametric estimation. The assessment of financing sources indicates that external debt has a more positive and significant effect on public investment efficiency than internal debt does.

Originality/value
This paper is unique in that it disentangles managerial efficiency from the technological efficiency of public investment in WEAMU countries and highlights how financing sources of investment affect its efficiency. In terms of policy implications, the underlying message of the results is that the rules and conditions of domestic or regional debt in the WAEMU countries must be strengthened to ensure better monitoring and then better efficiency of these resources.