bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of Molecular Interactions Between Fraser’s Photinia (Photinia X Fraseri) and Its Endophytic Bacterium PGB_Invit
Authors: TÜRKÖLMEZ Nil , KARAKAYA Merve , Ekinci Hamit , LUCAS Stuart J. , AKKAYA Özlem , Şeker Mine Gül , KAYIHAN Ceyhun , Ozden Yelda 
Published: February 2022

Abstract
An endophytic beneficial bacterium, was isolated and characterized from in vitro grown microshoots of Fraser’s Photinia in our previous studies. This bacterium, nominated as PGB_invit (Pseudorhodoplanes sp.), has ability to fix nitrogen and produce plant growth promoting regulators such as indole acetic acid and gibberellic acid. Probably because of all of these features, this specific bacterium promotes in vitro proliferation of Fraser’s Photinia microshoots and enables microshoots to be conserved at 25 ºC for up to 16 months without requiring periodic subculture. Due to its beneficial influence on growth of Fraser’s Photinia, it is aimed to identify physiological, biochemical and molecular influences of this bacterium together with determination of its the localization in plant tissues. Our results showed that inoculation with PGB_invit bacteria (106 cfu/ml) has increased chlorophyll content without causing any severe oxidative stress. Moreover, the genes [gene bank ID XP_008352422.1 (predicted “MID1-Complementing Activity 1”), PWZ54828.1 (Ubiquitin conjugating enzyme E2), XP_028948963.1 (predicted “E3 ubiquitin-protein ligase RHA1B-like”), XP_008339965.2 (uncharacterized protein), XP_028948163.1 (4-coumarate--CoA ligase-like 9), and XP_028953023 (methionine aminopeptidase 2B-like)] which are upregulated or downregulated after inoculation of this bacterium according to RNA-seq analysis, were also confirmed with qRT-PCR in order to understand its molecular interaction with its host.
“Struggle at Night – He Doesn’t Let Me Sleep Sometimes”: A Qualitative Analysis of Sleeping Habits and Routines of Hispanic Toddlers at Risk for Obesity
Authors: Gray Megan J. , Vazquez Christian , Agnihotri Ojasvie 
Published: February 2022

Abstract
Background: Hispanic children make up a growing percentage of all children in the U.S. and face disproportionately higher risks for early life obesity and resultant comorbidities such as Type 2 diabetes and fatty liver disease. Feeding and sleep habits are modifiable behaviors that impact early childhood obesity; Hispanic infants have been shown to have less nighttime sleep compared to their white counterparts. Pediatricians can coach families on parents’ nighttime responsive feeding and longer child sleep duration as protective factors against early life obesity, but must understand the family context and potential barriers. This study aimed to discover Hispanic parents’ motivations, goals, and ambivalence around nighttime feeding and sleep practices in infants and toddlers already at elevated risk for childhood obesity. By understanding family and social context, future recommendations can be tailored to the family’s needs to best implement healthy nighttime feeding and sleep behaviors.Methods: This qualitative study used a phenomenological approach. 14 Hispanic parents were recruited from a Federally Qualified Health Center in Central Texas for qualitative interviews regarding their experience raising a small child. Hispanic parents of children aged 6 to 18 months with child weight-for-length ratio ≥ 85% for age were recruited and consented during regular well child visits. Interviews occurred over several months during 2018-2019; Nvivo was used for analysis of qualitative themes. Multiple reviewers coded and used constant comparative methods to identify common themes. Results: Parents diverged from AAP recommended guidelines for infant and toddler feeding and sleep habits. Parents shared their intentions and the real-life barriers to implementing recommended habits. Co-sleeping, feeding to sleep, middle of the night feeding, and lack of structured sleep habits were common interview themes and potentially modifiable factors.Conclusions: Pediatricians need to be sensitive to culture and to the real-world needs of low-income families to determine if best practices are “practical.” Themes from these parent interviews can inform tailored interventions for children at high risk of obesity. Interventions should promote responsive nighttime feeding and structured sleep, working with individual family logistics, to coach families towards optimal healthy environments and healthy child weight.
pvSPS4 is Crucial for the Root Sugar Balance and Proper Tolerance Response in Salt-Tolerant Common Bean Genotype Under Salt Stress
Authors: Niron Harun , Türet Müge 
Published: February 2022

Abstract
Salinity is a stress factor that decreases global agricultural yield. Crops such as rice, tomato, potato, and legumes are susceptible to salt, thus this problem requires urgent attention. Stress-related carbohydrate metabolism is a dynamic network of genetic and metabolic pathways. Disruption in the regulation of soluble sugar content can have negative effects on source-sink partitioning. As source-sink partitioning is connected to the supply, and distribution of energy in the plant, it is critical for stress tolerance. Sucrose phosphate synthase (SPS) enzymes operate in a key regulatory step in sucrose synthesis. Therefore, they have a significant role in sugar metabolism including the regulation of source-sink sucrose transportation. This study focused on the function of the SPS homolog -pvSPS4- in the roots of legume crop common bean under salinity stress. We previously showed that pvSPS4 expression is root-specific and is upregulated under salt stress in a salt tolerant common bean genotype. This upregulation was accompanied by an accumulation of sugars in the roots. In the current study, using the same genotype, we generated composite common bean plants with wild-type shoot and pvSPS4 knock-down roots. Composite plants exhibited increased sensitivity to salt stress compared to control and mock plants. pvSPS4 knock-down disturbed the root glucose/sucrose ratio and ionic balance which resulted in a reduction in photosynthetic pigments together with osmoregulation and antioxidant capability. Our results imply that pvSPS4 is an important gene for carbohydrate balance regulation as a salt-stress response in the common bean root tissues.
Dynamics of Neutralizing Antibodies and Binding Antibodies to Domains of SARS-CoV-2 Spike Protein in COVID-19 Survivors
Authors: Phakaratsakul Supinya , Manopwisedjaroen Suwimon , Boonarkart Chompunuch , Kupatawintu Pawinee , Chaiwanichsiri Dootchai , Roytrakul Thaneeya , Auewarakul Prasert , Thitithanyanont Arunee 
Published: February 2022

Abstract
Neutralizing antibody level is used to predict immune protection against SARS-CoV-2 infection. Spike protein of SARS-CoV-2 is a major target for virus-neutralizing antibody. A number of neutralizing epitopes were mapped on receptor binding domain (RBD) and N-terminal domain (NTD) of S1 subunit of the spike. Anti-SARS-CoV-2 antibody usually decreases over time after recovery. Level of neutralizing antibody and binding antibody to several domains from COVID-19 recovered patients were observed longitudinally in this study. Sequentially collected serum samples from 35 patients demonstrated both similar and different trends of neutralizing antibodies versus binding antibodies to each domain. Twenty-three individuals showed similarly decreasing pattern of neutralizing titer, binding antibodies to RBD, NTD, fusion protein (S2) and nucleocapsid (NP). Interestingly, eight individuals had stably high neutralizing titer (≥320) for 3-12 months, while their binding antibodies to RBD, NTD and NP rapidly decreased. Moreover, their binding antibodies to S2 were stable over time similar to the persistence of neutralizing antibody levels. The longer-lasting antibody to S2 suggested an anamnestic response to cross-reactive epitopes from previous infections with other related coronaviruses. These data indicate a difference in kinetics and longevity of antibodies to various domains and epitopes of the SARS-CoV 2 proteins. A better understanding in this difference may help improve vaccine design to induce long-lasting immunity to COVID-19.
Combined Immunodeficiency Caused by a Novel De Novo Gain-of-Function RAC2 Mutation
Authors: Zhao Xiaodong , Zhang Liang , Chen Zhi , Li Wenyan , Liu Qiao , Wang Yanping , Chen Xuemei , Tian Zhirui , Yang Qiuyun , An Yunfei , Zhang Zhiyong , Mao Huawei , Tang Xuemei , Lv Ge 
Published: February 2022

Abstract
Ras-related C3 botulinum toxin substrate 2 (RAC2) is a GTPase exclusively expressed in hematopoietic cells, that acts as a pivotal regulator of several aspects of cell behavior via various cellular processes. RAC2 undergoes a tightly-regulated GTP-binding/GTP-hydrolysis cycle, enabling it to function as a molecular switch. Mutations in RAC2 have been identified in 18 patients with different forms of primary immunodeficiency, ranging from phagocyte defects caused by dominant negative mutations to common variable immunodeficiency resulting from autosomal recessive loss-of-function mutations, or severe combined immunodeficiency due to dominant activating gain-of-function mutations. Here, we describe an 11-year-old girl with combined immunodeficiency presenting with recurrent respiratory infections and bronchiectasis. Immunological investigations revealed low T-cell receptor extension circle/K-deleting recombination excision circles numbers, lymphopenia, and low serum immunoglobulin G. Targeted next generation sequencing identified a novel heterozygous mutation in RAC2, c.86C>G (p.P29R), located in the highly-conserved Switch I domain. The mutation resulted in enhanced reactive oxygen species production, elevated F-actin content, and increased RAC2 protein expression in neutrophils, as well as increased cytokine production and a dysregulated phenotype in T lymphocytes. Further, the dominant activating RAC2 mutation led to accelerated apoptosis with augmented intracellular active caspase 3, impaired actin polarization in lymphocytes and neutrophils, and diminished RAC2 polarization in neutrophils. We present a novel RAC2 gain-of-function mutation with implications for immunodeficiency and linked to functional dysregulation, including abnormal apoptosis and cell polarization arising from altered RAC2 expression. Thus, our findings broaden the spectrum of known RAC2 mutations and their underlying mechanisms.
User Fairness and QoS Aware based Effective Resource Allocation for Downlink NOMA Cellular Systems
Authors: GUPTA PRIYA , Ghosh D. 
Published: February 2022

Abstract
Non orthogonal multiple access (NOMA) is expected to be the most significant and viable solution for fifth generation (5G) communication system due to its incredible advantage of spectral efficiency. This paper proposes joint channel allocation and power optimization for downlink NOMA system, so as to maximize the system throughput while preserving the minimum data rate of every user equipment necessary to maintain quality of service and successive interference cancellation constraint. The optimization problem so formulated is a joint mixed integer nonlinear programming problem. Hence, two stage solution is proposed that uses mosek solver for channel assignment followed by power allocation across assigned channels. We use the difference between two convex functions (DC) programming technique for modifying the nonconvex optimization problem into a convex subproblem. Thus, we achieve efficient and fair power allocation coefficients across channels by solving the convex subproblem iteratively. Simulation results demonstrate better efficiency of our proposed scheme for resource allocation compared to some of the existing baseline schemes.
Practical Applications of Vitreous Imaging for the Treatment of Vitreous Opacities With YAG Vitreolysis
Authors: Jiang Shangjun , Golding John , Choudhry Netan 
Published: February 2022

Abstract
PurposeTo demonstrate the methodology and efficacy of using scanning laser ophthalmoscopy (SLO) and dynamic optical coherence tomography (OCT) to identify and treat symptomatic vitreous floaters using yttrium-aluminum garnet laser vitreolysis (YLV).MethodsThis is a case series highlighted from a cross-sectional retrospective study conducted at the Vitreous Retina Macula Specialists of Toronto.40 eyes from 35 patients were treated with YLV between November 2018 – December 2020 for symptomatic floaters and imaged with SLO and dynamic OCT. Patients were re-treated with YLV if they reported ongoing significant vision symptoms during follow-up which correlated to visible opacities on exam and or imaging.Three cases will be highlighted to present the practical applications of SLO and dynamic OCT imaging for YLV treatment. Results 40 treated eyes were enrolled in this study, with 26 eyes (65%) requiring at least one repeat YLV treatment following the first treatment due to ongoing symptomatic floaters. Case 1 demonstrates a dense, solitary vitreous opacity that has been localized with dynamic OCT imaging to track its movements and retinal shadowing with the patient’s eye movements. Case 2 shows the utility of adjusting the fixation target to monitor the movement of vitreous opacities in real-time. Case 3 exhibits an association between decreased symptom burden and vitreous opacity density after YLV.ConclusionImage-guided YLV facilitates the localization and confirmation of vitreous opacities. SLO and dynamic OCT of the vitreous can provide a real-time evaluation of floater size, movement, and morphology, to help clinicians target treatment and monitoring of symptomatic floaters.
Machine Learning Approach for Predicting Production Delays: A Quarry Company case study
Authors: kannan Rathimala , Halim Haq'ul Aqif bin Abdul , Ramakrishnan Kannan , Ismail Shahrinaz , Wijaya Dedy Rahman 
Published: February 2022

Abstract
Predictive maintenance employing machine learning techniques and big data analytics is a benefit to the industrial business in the Industry 4.0 era. Companies, on the other hand, have difficulties as they move from reactive to predictive manufacturing processes. The purpose of this paper is to demonstrate how data analytics and machine learning approaches may be utilized to predict production delays in a quarry firm as a case study. The dataset contains production records for six months, with a total of 20 columns for each production record for two machines. Cross Industry Standard Process for Data Mining approach is followed to build the machine learning models. Four predictive models were created using machine learning algorithms such as Decision Tree, Neural Network, Random Forest, and Nave Bayes. The results show that Multilayer Perceptron Neural Network outperforms other techniques and accurately predicts production delays with a sensitivity score of 0.979. The quarry company's improved decision-making reducing potential production line delays demonstrates the value of this study.
Modulatory Effects of Cilostazol; an Nrf2/HO-1 activator against NAFLD in Rats Confirmed by Molecular Docking and FTIR Studies
Authors: A.Sedik Ahmed , A.Amer Asmaa 
Published: February 2022

Abstract
Nonalcoholic fatty liver disease (NAFLD) is a complex hepato-metabolic syndrome with multi-etiological pathways. No effective drugs have been settled for the effective therapy of NAFLD. The purpose of this study was to investigate the modulatory effects of cilostazol (CILO, 50 and 100 mg/kg.p.o.) against NAFLD induced by high fat diet rich in cholesterol (HFD- CH) for 10 weeks. Thirty male Sprague daily rats, were divided into 4 groups (8 rat / group). Normal control group supplied with normal chow diet. Control positive group received high fat diet rich in cholesterol for 10 weeks. In addition, two CILO groups received (CILO, 50 and 100 mg/kg.p.o.). Oral administration of (CILO; 100 mg/kg) showed promising results in reducing fasting glucose and insulin levels. Moreover, CILO could reduce the elevated hepatic lipids, oxidative stress biomarkers and inflammatory cytokines. In addition, CILO succeeded to restore the total protein levels and activate nuclear factor erythroid-related factor 2/ heme oxygenase-1 (Nrf2/HO-1) activity. Furthermore, administration of CILO for NAFLD rats succeeded to show corrected and normalized FTIR spectra. We also investigated the plausible binding interactions of CILO with various biological targets using a molecular docking approach, and the results showed that CILO had an excellent docking energy score and significant binding interactions with the core amino acids involved in the active pocket for the enzymes studied. This study depicts that CILO exerted new intervention for NAFLD due to its complementary anti-hyperlipdemic, anti-inflammatory effects and antioxidant potential, via Nrf2/HO-1 activation.
Anatomy of Omicron neutralizing antibodies in COVID-19 mRNA vaccinees
Authors: Rappuoli Rino , Andreano Emanuele , Paciello Ida , Marchese Silvia , Donnici Lorena , Pierleoni Giulio , Piccini Giulia , Manganaro Noemi , Pantano Elisa , Abbiento Valentina , Pileri Piero , Benincasa Linda , Giglioli Ginevra , Leonardi Margherita , Maes Piet , De Santi Concetta , Sala Claudia , Montomoli Emanuele , De Francesco Raffaele 
Published: February 2022

Abstract
SARS-CoV-2 vaccines, administered to billions of people worldwide, are mitigating the effects of the COVID-19 pandemic, however little is known about the molecular basis of antibody cross-protection to emerging variants, such as Omicron (B.1.1.529), and other coronaviruses. To answer this question, 276 neutralizing monoclonal antibodies (nAbs), previously isolated from seronegative and seropositive donors vaccinated with BNT162b2 mRNA vaccine1, were tested for neutralization against the Omicron variant and SARS-CoV-1 virus. Cross-neutralizing antibodies were isolated from 100% of seropositive and 20% of seronegative vaccinees. Only 14.2% and 4.0% of tested antibodies neutralized the Omicron variant and SARS-CoV-1 respectively. These nAbs recognized mainly the SARS-CoV-2 receptor binding domain (RBD) and targeted class 3 and class 4 epitope regions on the SARS-CoV-2 spike protein. Antibodies targeting class 1/2 epitope regions only rarely showed cross-neutralization activity. Cross-protective antibodies derived from a variety of germlines, the most frequents of which were the IGHV1-58;IGHJ3-1 and IGHV1-69;IGHV4-1. Only 15.6% and 7.8% of predominant gene-derived nAbs elicited against the original Wuhan virus cross-neutralized Omicron and SARS-CoV-1 respectively. Our data provide evidence of the presence of cross-neutralizing antibodies induced by vaccination and map conserved epitopes on the S protein that can inform vaccine design.
Comparison of the Effect of Sexual Counseling Based on BETTER and PLISSIT Models on Sexual Assertiveness of Women with Breast Cancer after Mastectomy
Authors: shalamzari Kowsar Hakami , Asgharipour Negar , Jamali Jamshid , kermani Ali Taghizadeh , Babazadeh Raheleh 
Published: February 2022

Abstract
Introduction: Sexual changes in breast cancer occur after diagnosis and treatment include mastectomy. Sexual assertiveness is an effective factor in sexual satisfaction that means the ability to convey sexual feelings, beliefs, and thoughts and logically defend sexual rights. The present study aimed to compare the effectiveness of individual sexual counseling using the BETTER model with individual sexual counseling using the PLISSIT model in terms of increasing sexual assertiveness.Materials and Methods: The present quasi-experimental intervention was conducted in Mashhad in 2021, and 78 mastectomized women with breast cancer were randomly assigned to two groups: BETTER and PLISSIT. Both groups received four individual counseling sessions of 60-90 minutes one week apart. The research tools included demographic information form, female sexual function index (FSFI), and the Hulbert index of sexual assertiveness (HISA). Changes in the mean scores of sexual assertiveness between the two groups were evaluated before and 4 weeks after the intervention, and the mean changes were compared between the groups. Data analysis was conducted using the Kolmogorov-Smirnov test, independent t-test, paired t-test, and Chi-square tests using SPSS 25 (P<0.05).Results: The mean scores of sexual assertiveness were not significantly different between BETTER (46.7) and PLISSIT (43.07) groups before the intervention (P=0.253); however, the mean scores of sexual assertiveness in the BETTER group (54.8) and the PLISSIT group (48.6) were significantly different 4 weeks after the intervention. (P<0.001)Conclusion: The results indicated that sexual counseling based on the BETTER model was more effective in increasing the sexual assertiveness of mastectomized women with breast cancer than counseling by the PLISSIT method.
Earlier CRRT is Associated with Reduced Mortality in Rhabdomyolysis Patients
Authors: Li Xiayin , Bai Ming , Yu Yan , Ma Feng , Zhao Lijuan , Li Yajuan , Wu Hao , Sun Shiren 
Published: February 2022

Abstract
Background: Continue renal replacement therapy (CRRT) is commonly employed for rhabdomyolysis (RM) patients. However, the optimal initiation timing of CRRT and prognostic factors were not well evaluated for patients with RM. We aimed to investigate the efficacy of CRRT timing on mortality and the risk factors of death in RM patients who accepted CRRT.Methods: RM patients who received CRRT between May 2010 and May 2021 in our center were retrospectively included. The primarily endpoint was 90-day mortality. Univariate and multivariate logistic analyses were performed to identify the risk factors of 90-day mortality.Results: A total of 134 patients were included in our present study. The 90-day mortality rate was 38.06%. Most of the patients (88.81%) reached peak creatine kinase (CK) within 72 hours after admission, and 58 (43.28%) patients received CRRT before the peak CK occurrence (earlier CRRT) and 76 (56.72%) of patients had CRRT after the peak CK occurrence (later CRRT). Multivariate logistic regression analysis showed that CRRT initiation after the peak CK occurrence (OR = 3.74, 95%CI 1.17-11.95, p = 0.026), the elevated serum cTnI (OR = 1.23, 95%CI 1.02-1.49, p = 0.032), and the need of mechanical ventilation support (OR = 7.18, 95%CI 1.67-30.83, p = 0.008) were independent risk factors of 90-day mortality. Similar results were obtained in a subgroup analysis of patients with acute kidney injury (AKI).Conclusions: Earlier CRRT initiation before the peak CK occurrence was associated with lower 90-day patient mortality.
Modulation instability gain and Modulated wave shape incited by the acoustic longitudinal vibrations in Molecular Chain Model
Authors: Houwe Alphonse , Djorwe Philippe , Saliou Youssoufa , Abbagari Souleymanou , Doka Serge Yamigno 
Published: February 2022

Abstract
The propagation of the modulated wave pattern and its instability in nonlinear medium is an important study from where experimental results are obtained. In this work we use the Nonlinear Schr\"{o}dinger Equation (NLSE) with saturation nonlinearity which describes the solitonic waves in molecular chain model. We set our study on the effectiveness of the acoustic longitudinal velocity which is related to the acoustic longitudinal vibration to investigate the behavior of the modulated wave patterns as well as the modulation instability (MI) gain. Its results that the acoustic longitudinal velocity creates instability zones, increases MI bands and shortens the transition regime of the modulated wave pattern. For long time of simulation and great enough value of the acoustic longitudinal velocity the modulated waves feature chaos-like motion. Alongside of these results the acoustic longitudinal velocity obvious itself as tool control of modulated wave as well as an energy source for the propagation of diverse modulated wave shaped in molecular chain.