bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Late Surfactant Treatment Delivered After a Recruitment Maneuver on Respiratory Outcomes in Extremely Low Gestational Age Newborns: A Randomized Controlled Pilot Trial – LATE-REC-SURF Trial.
Authors: Lio Alessandra , Tirone Chiara , Tana Milena , Aurilia Claudia , Blandino Rita , De Tomaso Davide , Fusco Francesca , Paladini Angela , Nobile Stefano , Perri Alessandro , Cota Francesco , Pastorino Roberta , Vento Giovanni 
Published: January 2022

Abstract
Background
Mechanical ventilation is still needed in most preterm newborns, even in the non-invasive ventilation era. Ventilator-induced lung injury is one of the known pathogenetic factors of bronchopulmonary dysplasia (BPD) in preterm newborns. Lung injury has several patterns including surfactant dysfunction. Some recent trials have showed that a late surfactant administration can improve respiratory outcome in preterm babies still on invasive ventilation after the first week of life. Unfortunately, these results are still not conclusive. Moreover, giving surfactant after a recruitment manoeuvre in High Frequency Oscillatory Ventilation (HFOV) was shown to be safe and to reduce mortality in extremely preterm infants in a recent RCT. Our aim is to test the hypothesis that endotracheal administration of poractant-alfa preceded by a recruitment manoeuvre in HFOV in preterm infants still requiring mechanical ventilation at 7-10 days of life could facilitate extubation.
Methods/Design:
This will be an unblinded monocentric pilot trial that will be conducted in a III level Neonatal Intensive Care Unit at Fondazione Policlinico Agostino Gemelli IRCCS in Rome - Italy. Preterm newborns with a gestational age < 28 weeks still requiring invasive mechanical ventilation at 7-10 days of life with a fraction of inspired oxygen (FiO2) of more than 0.30 and/or an oxygenation index of 8 or more for at least 6 hours will be eligible for the study. Patients will be randomly assigned to intervention or to standard care. Intervention group infants will receive up to 4 doses of Poractant-alfa every 12 hours, each dose preceded by a recruitment manoeuvre in HFOV, until extubation. Primary endpoint will be the first successful extubation.
Discussion
Surfactant therapy is nowadays recommended in case of RDS in the first days of life but little is known about its effects in ventilator-dependant preterm newborns. Late administration of surfactant could help healing the lung of preterm babies in which RDS is evolving in a chronic pulmonary insufficiency of prematurity. The findings of this pilot trial will permit evaluation of the study design for a full-scale RCT.
Trial registration:
Clinicaltrials.gov – ID NCT04825197. Registered 12 April 2021, https://clinicaltrials.gov/ct2/home
A Simplified Formula for the Determination of the Fundamental Period of Mixed Structures with Vertical Combination of Different Seismic Resisting Systems
Authors: Saadatkhah Amir , Chenaghlou Mohammad Reza , Poursha Mehdi 
Published: January 2022

Abstract
Vertical combination of different seismic resisting systems, permitted in the seismic design codes such as ASCE/SEI 7-16, is an effective choice for the structural design of mid- and high-rise buildings. In the seismic analysis of structures, according to the seismic codes, an estimate of the fundamental period of vibration is needed. On other hand, there is no simplified equation to compute the fundamental period of vibration of mixed buildings with vertical combination of different seismic resisting systems. In this study, by using the Rayleigh’s method, a simplified equation for determining the fundamental period of this type of structures is proposed. This equation is verified by the eigenvalue analysis of the mathematical model of the 11 building frames with different seismic characteristics along their height. Furthermore, two different mixed structures composed of steel and reinforced concrete moment resisting frame (MRF) structures as well as of steel (concentrically braced frame) CBF and reinforced concrete MRF structures are examined in this paper. The results indicate that the proposed formula results in the fundamental period of mixed structures with satisfactory accuracy. The largest and average errors in the fundamental period of the buildings under consideration, obtained by the proposed formula, are less than 10% and 6%, respectively, compared to the eigenvalue analysis.
A More Important Role of Diabetes Mellitus in Mortality in Elderly Critical COVID-19 Patients: a Single-center Retrospective Study
Authors: Li Yuming , Li Xiantao , Zhou Yu , Yang Yi , Yao Yake , Zhou Hua , Hu Dakang 
Published: January 2022

Abstract
BackgroundCOVID-19 has been circulating worldwide since December 2019. However, its independent risk factors of mortality need further insights in elderly critical COVID-19 patients.MethodsTotally 48 elderly and critically ill COVID-19 patients with clear end point were enrolled when the data were collected, with 16 discharged and 32 died. Kaplan-Meier analysis and Cox regression were performed to identify the risk factors of mortality in elderly critical COVID-19 patients. Survival curve was conducted to present the impact of Diabetes Mellitus on mortality. Mann-Whitney U and t tests were used to 39 clinical variates between the survivor and non-survivor groups.ResultsAs Kaplan-Meier analysis confirmed, only three variates Diabetes Mellitus, chronic obstructive pulmonary disease and family aggregation showed significant difference between the survivor and non-survivor groups. However, only variate Diabetes Mellitus presented significance in Cox regression. Higher C-reaction protein, interleukin-2 (IL-2), IL-8 and creatinine were detected in survivor group than non-survivor group, which was reverse to estimated glomerular filtration rate. The other laboratory finding showed no significant difference between survivor and non-survivor groups.ConclusionsDiabetes Mellitus, chronic obstructive pulmonary disease and family aggregation can contribute to mortality by COVID-19 while Diabetes Mellitus also reduces the survival time of elderly and critically ill COVID-19 patients, highlighting the more important role of Diabetes Mellitus. Laboratory findings could not serve as good predictors of death for elderly and critically ill COVID-19 patients.
Shenmai Injection Exerts Neuroprotective Functions by Down-regulating MicroRNA-19a in H 2 O 2 -induced PC12 Cells
Authors: Wu Jing , li zhonghao , dong xiaoke , yuan siyuan , liu jinmin , wang le 
Published: January 2022

Abstract
Background: Acute ischemic stroke (AIS) and following reperfusion therapy-induced cerebral ischemia reperfusion (I/R) injury have been recognized as an important subject of cerebrovascular disease with high mortality. Oxidative stress is an important pathological process of cerebral I/R injury. microRNA-19a (miR-19a) is involved in I/R. As the organ protectant agent, Shenmai Injection (SMI) is widely used in the clinical treatment of cerebral infarction. Purpose: This study aims to explore whether SMI can reduce oxidative stress by regulating miR-19a, thereby treating I/R injury. Methods: The oxidative stress state of PC12 cells was induced by H2O2, and then the cells were cultured with SMI. The therapeutic effect of SMI was evaluated by detecting cellular superoxide dismutase (SOD), malondialdehyde (MDA) and other oxidative markers with the kit. Western blot, PCR, immunofluorescence and other techniques were used to elucidate the potential mechanism of SMI. Results: Cell viability assay results showed that SMI could improve the viability of PC12 cells stimulated by H2O2. Compared with the H2O2 group, after SMI treatment, the contents of MDA and reactive oxygen species (ROS) were significantly reduced, while the activity of SOD was significantly increased, and SMI could reduce apoptosis by increasing the content of adenosine 5'-triphosphate (ATP) in cells and enhancing the mitochondrial membrane potential (∆Ψm). Western blot and qRT-PCR results showed that these effects were partially achieved through the AMPK/Sirt1/PGC-1α pathway. The level of miR-19a was significantly increased in H2O2 group, and SMI could protect the cells by reducing miR-19a. Further investigated the target of miR-19a, and transfected cells with miR-19a mimic and inhibitor respectively. We found that AdipoR2 was a direct target of miR-19a, and miR-19a could inhibit AdipoR2/PI3K/Akt/mTOR pathway. Conclusion:SMI can activate AMPK/Sirt1/PGC-1α and AdipoR2/PI3K/Akt/mTOR pathways by reducing miR-19a levels, and protect PC12 cells stimulated by H2O2.
High Glucose-Induced Vascular Smooth Muscle Cell Proliferation and Migration are Regulated by the miR-34a-Notch1 Pathway
Authors: Zhang Liping , Pan Rongrong , Zhang Qing , Gu Linling , Shi Gongde , Wu Yutao 
Published: January 2022

Abstract
High glucose(HG)-induced excessive proliferation and migration of the media vascular smooth muscle cell(VSMC) are the main pathological characteristics in diabetes related vascular injuries. Previous studies have shown that microRNA-34a (miR-34a) is involved in cancer metastasis, proliferation and invasion and plays an essential role in cardiovascular disease. However, little is known about the regulating role miR-34a in HG-induced proliferation and migration of VSMC. Here we demonstrated that miR-34a was downregulated at different timepoints under HG stimulation. Then, HG induced proliferation and migration was found to be impaired by miR-34a overexpression using transwell, CCK8 and RT-qPCR assays. Furthermore, the HG-induced depression of “contractile” VSMC-specific markers were reversed by the overexpression of miR-34a. Moreover, we confirmed that miR-34a regulated HG-induced VSMC proliferation and migration through its target gene, Notch1, which has been shown to be associated with cell proliferation and migration in previous studies. Taken together, we propose that the miR34a-Notch1 axis plays an important role in regulating HG-induced VSMC proliferation and migration.
Preparation of Blueberry Anthocyanin Nanoemulsion And Its Stability and Skin Safety Evaluation
Authors: YIN Mingyue , SONG Jieyu , ZHOU Xiaoling , ZHANG Wei , WU Ao 
Published: January 2022

Abstract
BACKGROUND: Blueberry anthocyanins have strong antioxidant activity, but their instability and low bioavailability limit their use. Nanoemulsion is a new type of food and drug carrier with stable thermodynamic properties. Therefore, anthocyanins will be encapsulated with nanoemulsion to improve its stability and application value.RESULTS: In this study, the best surfactants, oil phases and cosurfactants for the preparation of blueberry anthocyanin nanoemulsion were screened by pseudo-ternary phase diagram method and the solubility of blueberry anthocyanin nanoemulsion was taken into consideration. Deep purplish red, clear and transparent anthocyanin nanoemulsion was prepared by simple and cheap low-energy emulsification method according to the formula. Normal and high temperature test and high speed centrifugal test proved that the blueberry anthocyanin nanoemulsion had good stability. Guinea pig skin irritation test and sensitization test showed that there was no irritation and sensitization to guinea pig hair removal skin. CONCLUSION: The blueberry anthocyanin nanoemulsion prepared in this study has good stability at room temperature and is safe for guinea pig hair removal skin. It provides a basis for improving the stability and bioavailability of blueberry anthocyanin, and provides a reference for the application of blueberry anthocyanin nanoemulsion in skin beauty and transdermal drug delivery.
Effect of Different Preparation Conditions On The Properties of Bamboo Fiber-Based Bioactive Composite Membrane
Authors: Liuyun Jiang , Ma Bingli , Jiang Zhihong , Ma Yingjun , Wen Yue , Zhang Na , Zhang Yan , Su Shengpei , Tang Xionggui , Hu Xiang 
Published: January 2022

Abstract
A novel nano-hydroxyapatite/bamboo fiber (n-HA/BF) bioactive composite membrane was obtained by a simple casting technique. The membrane forming mechanism and the effects of different forming membrane methods, drying methods and n-HA amounts on the properties of n-HA/BF membrane were investigated by Fourier Transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), contact angle, electromechanical universal tester, in vitro soaking in simulated body fluid (SBF) and in vitro cell cultureexperiment. The results demonstrated that the n-HA dispersity in BF matix was not affected by the prepartion condition, however, the morphologies of membrane was determined by the different preparation conditions owing to different hydrogen bond shrinkage. Moreover, the hydrophilicity of the composite membrane was improved under the condition of the membrane formation in oven, freeze drying and high addition content of n-HA, and the mechanical properties of composite membrane depended on n-HA content. In vitro soaking behavior indicated that the degradability and bone-like apatite deposition could be controled by differentpreparation conditions. And the cell proliferation experiment showed that the n-HA/BF composite membranes obtained under different preparation conditions were all non-toxic. The above results indicated that the n-HA/BF composite membrane could be obtained by a simple casting technique, and the properties could be controlled by adopting different preparation conditions, which would have a great promising as guide bone tissue regeneration (GBR) membrane, and the study would provide a new application for BF in biomedical field.
Comparative Proteomics Reveals Differential Mechanism of Root Cold-resistance between Vitis. Riparia × V. Labrusca and Cabernet Sauvignon
Authors: Chen Si-jin , Su Hong-yan , Xing Hua , Yang De-long , Mao Juan , Sun Ping , Li Meng-fei 
Published: January 2022

Abstract
Grapevines, containing large amounts of bioactive metabolites that offer health benefits, are widely cultivated around the world. The cold damage of growing outside with extreme low temperature during overwintering stage limits the expansion of production. Although the levels of morphological, biochemical and molecular in different Vitis species exposure to different temperatures have been investigated, differential expression of proteins in roots is still limited. Here, the roots of cold-resistant (Vitis. riparia × V. labrusca, T1) and cold-sensitive varieties (Cabernet Sauvignon, T3) at −4°C as well as of the former at −15°C (T2) were measured by iTRAQ-based proteomic analysis, expression levels of genes encoding candidate proteins were validated by qRT-PCR. The results showed that the root activity of cold-resistant variety was stronger than that of cold-sensitive variety, and it declined with the decrease of temperature. A total of 25 proteins were differentially co-expressed at T2 versus (vs) T1 and T1 vs T3, and these proteins were involved in stress response (e.g. DHN1, SHSPCP and USPCP), bio-signaling (e.g. PKCP, S/TPP and nsS/TP), metabolism (e.g. GluP, GluBE and PE), energy (e.g. AAC, AAACP and NADCP), and translation (e.g. rpL14, rpS21 and PPI). The relative expression levels of the candidate 13 genes were consistent with their fold-change values of proteins. The signature translation pattern for the roots at spatio-temporal treatments of varieties and temperatures provides insight into the differential mechanism of cold resistance of grapevines.
Gray Matter Atrophy And Corresponding Impairments In Connectivity In Patients With Anti-N-Methyl-D-Aspartate Receptor Encephalitis
Authors: Guo Yuanyuan , Lv Xinyi , Zhang Juanjuan , Li Chenglong , Wei Ling , Zhou Nong , Xu Jinping , Tian Yanghua , Wang Kai 
Published: January 2022

Abstract
Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is a severe autoimmune disease that is commonly accompanied by cognitive impairment and various neurological and psychiatric symptoms, advanced image analyses help explore the pathogenesis of this disease. Therefore, this study aimed to explore specific structural and functional alterations and their relationship with the clinical symptoms of anti-NMDAR encephalitis. In this study, twenty-two patients with anti-NMDAR encephalitis after the acute stage and 29 controls received cognitive assessments and magnetic resonance imaging. Grey matter atrophy was measured using voxel-based morphometry, and functional alterations in abnormal regions were subsequently investigated using resting state functional connectivity (RSFC). Finally, correlation analyses were performed to explore the associations between imaging alterations and cognitive assessments. The patients demonstrated significant gray matter atrophy in the bilateral triangle part of the inferior frontal gyrus (triIFG.L and triIFG.R) and right precuneus, decreased RSFC between triIFG.L and bilateral Heschl gyrus (HES), decreased RSFC between triIFG.R and HES.R, decreased RSFC between right precuneus and left cerebellum, and increased RSFC between triIFG.R and left superior frontal gyrus. Further correlation analyses showed that the gray matter volume in triIFG.R and decreased RSFC between triIFG.L and HES.R were associated with decreased memory scores, whereas decreased RSFC between triIFG.R and HES.R was marginally correlated with the disease course in patients. In conclusion, this study suggests that cognitive impairments in patients with anti-NMDAR encephalitis may be mainly associated with gray matter atrophy and abnormal RSFC in the triIFG. These findings provide new insights into anti-NMDAR encephalitis pathogenesis and help explore potential treatments.
Prognostic Value of Tumor-stroma Ratio in Oral Squamous Cell Carcinoma: Contribution of Cancer Associated Fibroblasts
Authors: Qiu Jingjian , Jiang Erhui , Shang Zhengjun 
Published: January 2022

Abstract
Background: The aim of this study is to confirm the prognostic value of the tumor–stroma ratio (TSR) in a large cohort of oral squamous cell carcinoma (OSCC) and further demonstrated the cancer associated fibroblasts (CAFs)-stroma ratio (CSR) served as a critical biomarkers contributed to the prognostic value of TSR Results: The threshold level of TSR value is 50%, which divides patients into high (>50%) and low (<50%) stroma. We examined the TSR on hematoxylin and eosin-stained tissue samples from 581 patients with oral squamous cell carcinoma and 298 cases were included in the high-stroma group. In multivariate analysis, the TSR was identified as an independent prognostic factor for disease-free survival (DFS) (hazard ratio (HR), 2.11; 95% confidence interval (CI), 1.56–2.86; p < 0.001) and oral cancer-specific survival (OCSS) (HR, 2.56, 95% CI, 1.78–3.67; p < 0.001). The interaction term reached statistical significance for histological grade. Multivariate analysis confirmed the discriminative value of the TSR in well differentiated tumors for DFS and OCSS separately (P=0.001, P=0.003). The prognostic value of TSR was not varied by other clinically subgroups. Furthermore, the high-stroma group had a higher Fibroblast Activation Protein (FAP+) CSR and α-Smooth Muscle Actin (α-SMA+) CSR than the low-stroma group (p < 0.001).Conclusion: High-stroma levels indicated a negative consequence and a higher CAFs–stroma ratio than low-stroma levels in OSCC. The TSR is not altered by other clinically elements rendering it a credible histological parameter and informing the rational design of individual cancer management.
Silent Death by Sound: C60 Fullerene Sonodynamic Treatment of Cancer Cells
Authors: Radivoievych Aleksandar , Kolp Benjamin , Grebinyk Sergii , Prylutska Svitlana , Ritter Uwe , Zolk Oliver , Glökler Jörn , Frohme Marcus , Grebinyk Anna 
Published: January 2022

Abstract
The acoustic pressure waves of ultrasound (US) penetrate biological tissues deeper than light. Another important feature of US its potential to generate light emission within the excited medium termed sonoluminescence. This promoted the idea of its use as an alternative energy source for photosensitizer excitation. Pristine C60 fullerene (C60), an excellent photosensitizer, was explored in the frame of cancer sonodynamic therapy (SDT). For that purpose, we analyzed C60 effects on human cervix carcinoma HeLa cells in combination with a low intensity US treatment. The time-dependent accumulation of C60 in HeLa cells reached its maximum at 24 h (800 ± 66 ng / 106 cells). Half of extranuclear C60 localized within mitochondria. The efficiency of C60 nanostructure’s sonoexcitation with 1 MHz US was tested with cell viability assay. A significant proapoptotic sonotoxic effect was found for HeLa cells. C60’s ability to induce apoptosis of carcinoma cells after sonoexcitation with US provides a promising novel approach for cancer treatment.
Extracellular Vesicles from Carcinoma-Associated Fibroblasts Promote Epithelial-Mesenchymal Transition of Salivary Adenoid Cystic Carcinoma via Interleukin-6
Authors: Kong Jing , Ran Yan , Zhu Lei , Li Jiao , Liu Xue , Yang Xuesong , Qi Dongyuan , Liu Tingjiao 
Published: January 2022

Abstract
Carcinoma-associated fibroblasts (CAF) play a pivotal role in cancer progression. Salivary adenoid cystic carcinoma (SACC) has a high tendency to invade and metastasize. Understanding how CAF interact with SACC cells is essential to develop new targeting therapies for SACC. Extracellular vesicles (EVs) play important roles in intercellular communication. However, the role of CAF-derived EVs in SACC invasion remains poorly understood. In this study, we show that CAF EVs promote the migration and invasion abilities of SACC cells. The expression of biomarkers of epithelial-mesenchymal transition (EMT) was higher in SACC cells treated with CAF EVs treated with CAF EVs than in the negative controls, and high levels of IL-6 were detected in CAF and their EVs. Knockdown of IL-6 in CAF decreased invasive abilities and EMT biomarker expression in SACC cells induced by CAF EVs. CAF EV-associated IL6 promoted SACC EMT by activating JAK2/STAT3 signaling pathway. These findings suggest that targeting CAF-derived EVs may be an effective strategy for inhibiting SACC invasion.
A New Chemical-Mechanical Polishing Method Based On Colloids Silica and MgO Was Developed For Polishing Y3Al5O12 Material
Authors: Duc Le Anh , Hieu Pham Minh , Quang Nguyen Minh 
Published: January 2022

Abstract
The material yttrium aluminum oxide (Y3Al5O12) is one of the materials commonly used in laser devices. For application in optical devices, it is necessary to produce ultra-precise surface quality, however, Y3Al5O12 material belongs to the group of difficult-to-machine materials with high brittleness and hardness. Therefore, it is very difficult to ensure that the main criterion when finishing this material to produce a quality surface in the nanometer form with the ability to remove the material is very difficult. To solve this problem, this work provided a new chemical - mechanical polishing mixture. The proposed polishing mixture of ZrO2, Na2SiO3–5H2O, and MgO abrasives has a weight ratio of 8%, 5% and 1% respectively, with the remainder being deionized water. The surface result after polishing is obtained with a material removed rate of 38 (nm/min) along with an ultra-smooth surface produced with Ra = 0.41 nm. With the help of X-ray photoelectron spectroscopy (XPS) method before and after polishing by CMS, the reaction mechanisms were elucidated. Analytical results show that Y3Al5O12 material produces YOOH and AlOOH in Na2SiO3 solution, then combines with –Si–OH to form (Y-Si) and (Al-Si) with significantly reduced hardness compared to other Y3Al5O12 materials, these products combine with MgO to form montmorillonites (3MgO–Al2O3–3SiO2–3Y2O3–5Al2O3). With this formation, the surface layer of Y3Al5O12 material becomes soft and is easily removed by ZrO2 abrasive particles under the influence of mechanical polishing, resulting in superfine surfaces are generated from the proposed CMS model.