bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the implementation and delivery of primary care services for transgender individuals in Ontario: case study protocol
Authors: Ziegler Erin , Valaitis Ruta , Carter Nancy , Risdon Cathy , Yost Jennifer 
Published: December 2022

<p>Background: Historically transgender adults have experienced barriers in accessing primary care services. In Ontario, Canada, health care for transgender adults is accessed through primary care; however, a limited number of practitioners provide care, and patients are often waiting and/or traveling great distances to receive care. The purpose of this protocol is to understand how primary care is implemented and delivered for transgender adults. The paper presents how the case study method can be applied to explore implementation of health services delivery for the transgender population in primary care. Methods: Case study methodology will be used to explore this phenomenon in different primary care contexts. Normalization Process Theory is used as a guide. Three cases known to provide transgender primary care and represent different Ontario primary care models have been identified. Comparing transgender care implementation and delivery across different models is vital to understanding how care provision to this population can be supported. Qualitative interviews will be conducted. Participants will also complete the NoMAD (NOrmalization MeAsure Development) survey, a tool measuring implementation processes. The tool will be modified to explore the implementation of primary care services for transgender individuals. Documentary evidence will be collected. Cross-case synthesis will be completed to compare the cases. Discussion: Findings will provide an Ontario perspective on the implementation and delivery of primary care for transgender adults in different primary care models. Results may be applicable to other primary care settings in Canada and other nations with similar systems. Barriers and facilitators in delivery and implementation will be identified. Providing an understanding and increasing awareness of the implementation and delivery of primary care may help to reduce the invisibility and disparities transgender individuals experience when accessing primary care services. Understanding delivery of care could allow care providers to implement primary care services for transgender individuals, improving access to health care for this vulnerable population.</p>
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Object Classification and Tracking Using Scaled P8 YOLOv4 Lite Model
Authors: Shaikh Shakil , Chopade Jayant , Kharate Gajanan 
Published: December 2022

One of the most difficult tasks in the area of computer vision is object detection, which combines object categorization and object location within a scene. In terms of object detection, Deep Neural Networks have been recently demonstrated to outperform alterna-tive approaches. The issues related deep learning neural network is its complexity and huge computation, so it is not possible to detect and track the objects in image of high resolution in real time. We proposed scaled YOLOv4 lite model as Single Stage Detector Neural Network for object detection, tracking and it is trained using COCO 2017 dataset. To create the YOLOv4-CSP- P5- P6- P7- P8 networks, the Scaled YOLOv4 applied efficient network scaling strategies. The additional layer in YOLOv4 lite model is added as P8 layer which improves accuracy. Cross-stage-partial (CSP) connections and Mish activation are used in improved network design, such as backbone optimization and Neck (PAN). In the case of YOLOv4, however, it can only be trained once for all resolutions. Width and Height activations have been changed, allowing for faster network training. With YOLOv4 lite model, we used CSPDarkNet-53 model as a backbone. The experimental result show our YOLOv4 lite model can detect and track object up to 28 fps when model run with the video input and has an accuracy of 86.09% when tested on real-time video with resolutions 1920 × 1080 (full HD). AP = 50.81%, AP @50 = 63.6%, and AP @75 = 52.5% for CSPDarkNet-53 model backbone.
Rancang Bangun Aplikasi Kasir Penjualan pada Usaha Mikro Kecil Mengengah 3Manstore Berbasis Web
Authors: Mulyani Asri , Setiawan Ridwan , Rusmana Ruli Ahmad 
Published: December 2022

Aplikasi kasir penjualan pada usaha mikro kecil menengah merupakan jawaban terhadap permasalahan yang ada pada salah satu usaha mikro kecil menengah pengrajin tas yang berada di daerah Leles Kabupaten Garut. Hasil dari observasi dan wawancara dengan pemilik usaha mikro kecil menengah permasalahan yang ada adalah proses transaksi masih manual menggunakan nota tulis sehingga sangat riskan untuk nota hilang atau rusak, proses perhitungan laba yang kadang tidak balance dikarenakan human error saat proses rekapitulasi serta tidak adanya catatan hutang sehingga saat proses pembayaran hutang reseller kadang tidak sinkron. Tujuan dibuatnya aplikasi ini adalah untuk membantu proses kegiatan penjualan pada usaha mikro kecil menengah, membantu mengelola hutang piutang, dan membantu proses rekapitulasi laporan laba rugi. Rational Unified Process (RUP) yang di mana tahapan pelaksanaannya hanya meliputi inception, elaboration, dan construction menjadi bagian dari metodologi yang digunakan. Kemudian mengguinakani pemodnelan Uinifbieid Miodeilling Lainguaige dan penigujian alpha dengan menggunaiikan metoidie Blacik Box Tiesiting dilakukan pada tahapan construction, sedaingkan uintuk bahiasaipemiograman ialah PHP dan MiySql sebiagai basis datanya. Hasil dari peneliatian ini berupa Aplikasi Kasir Penjualan Untuk Usaha Mikro Kecil Menengah Berbasis Web yang memilih hak akses multi-user serta sudah dilengkapi dengan fitur transaksi, manajemen hutang dan laporan laba rugi.
EFFECT OF SERVICE QUALITY ON CUSTOMER SATISFACTION AT METSKY DINE & LOUNGE BEKASI
Authors: Ishak Riani P. , Utami Nisa Rahmaniyah 
Published: December 2022

The purpose of this study was to determine and analyze the effect of service quality on customer satisfaction at Metsky Dine & Lounge Bekasi. This research uses descriptive research with a quantitative approach and simple linear regression analysis method. The sampling technique uses accidental sampling. The population is all customers who come to Metsky Dine & Lounge Bekasi, while the total sample is 100 respondents based on the Slovin formula. The results showed that the service quality variable has an effect on customer satisfaction at the Metsky Dine & Lounge Bekasi restaurant. It is hoped that Metsky Dine & Lounge Bekasi will continue to maintain and improve their service quality so customers continue to feel satisfied.
Scaling-up digital follow-up care services: collaborative development and implementation of Remote Patient Monitoring pilot initiatives to increase access to follow-up care
Authors: Azevedo Salomé , Guede-Fernández Federico , von Hafe Francisco , Dias Pedro , Lopes Inês , Cardoso Nuno , Coelho Pedro , Santos Jorge , Fragata José , Vital Clara , Semedo Helena , Gualdino Ana , Londral Ana 
Published: December 2022

BackgroundCOVID-19 increased the demand for Remote Patient Monitoring (RPM) services as a rapid solution for safe patient follow-up in a lockdown context. Time and resource constraints resulted in unplanned scaled-up RPM pilot initiatives posing risks to the access and quality of care. Scalability and rapid implementation of RPM services require social change and active collaboration between stakeholders. Therefore, a participatory action research (PAR) approach is needed to support the collaborative development of the technological component while simultaneously implementing and evaluating the RPM service through critical action-reflection cycles.ObjectiveThis study aims to demonstrate how PAR can be used to guide the scalability design of RPM pilot initiatives and the implementation of RPM-based follow-up services.MethodsUsing a case study strategy, we described the PAR team’s (nurses, physicians, developers, and researchers) activities within and across the four phases of the research process (problem definition, planning, action, and reflection). Team meetings were analyzed through content analysis and descriptive statistics. The PAR team selected ex-ante pilot initiatives to reflect upon features feedback and participatory level assessment. Pilot initiatives were investigated using semi-structured interviews transcribed and coded into themes following the principles of grounded theory and pilot meetings minutes and reports through content analysis. The PAR team used the MoSCoW prioritization method to define the set of features and descriptive statistics to reflect on the performance of the PAR approach.ResultsThe approach involved two action-reflection cycles. From the 15 features identified, the team classified 11 as must-haves in the scaled-up version. The participation was similar among researchers (52.9%), developers (47.5%), and physicians (46.7%), who focused on suggesting and planning actions. Nurses with the lowest participation (5.8%) focused on knowledge sharing and generation. The top three meeting outcomes were: improved research and development system (35.0%), socio-technical-economic constraints characterization (25.2%), and understanding of end-user technology utilization (22.0%).ConclusionThe scalability and implementation of RPM services must consider contextual factors, such as individuals’ and organizations’ interests and needs. The PAR approach supports simultaneously designing, developing, testing, and evaluating the RPM technological features, in a real-world context, with the participation of healthcare professionals, developers, and researchers.
Estimating causal effects of genetically predicted type 2 diabetes on COVID-19 in the East Asian population
Authors: Yoshikawa Masahiro , Asaba Kensuke , Nakayama Tomohiro 
Published: December 2022

BackgroundObservational studies suggested that type 2 diabetes mellitus (T2DM) was associated with an increased risk of coronavirus disease 2019 (COVID-19). However, Mendelian randomization (MR) studies in the European population failed to find causal associations, partly because T2DM was pleiotropically associated with body mass index (BMI). We aimed to estimate the causal effects of T2DM on COVID-19 outcomes in the East Asian (EAS) population using a two-sample MR approach.MethodsWe obtained summary statistics from a genome-wide association study (GWAS) that included 433,540 EAS participants as the exposure dataset for T2DM risk and from COVID-19 Host Genetics Initiative GWAS meta-analyses (round 7) of EAS ancestry as the outcome dataset for COVID-19 susceptibility (4,459 cases and 36,121 controls), hospitalization (2,882 cases and 31,200 controls), and severity (794 cases and 4,862 controls). As the main MR analysis, we performed the inverse variance weighted (IVW) method. Moreover, we conducted a series of sensitivity analyses, including IVW multivariable MR using summary statistics for BMI from a GWAS with 158,284 Japanese individuals as a covariate.ResultsThe IVW method showed that the risk of T2DM significantly increased the risk of COVID-19 susceptibility (odds ratio [OR] per log (OR) increase in T2DM, 1.11; 95% confidence interval [CI], 1.02–1.20; P = 0.014) and hospitalization (OR, 1.15; 95% CI, 1.04–1.26; P = 0.005), although the risk of severity was only suggestive. Moreover, IVW multivariable MR analysis indicated that the causal effects of T2DM on COVID-19 outcomes were independent of the effect of BMI.ConclusionsOur MR study indicated for the first time that genetically predicted T2DM is a risk factor for SARS-CoV-2 infection and hospitalized COVID-19 independent of obesity in the EAS population.
SIRT1 haplo-insufficiency results in reduced cortical bone thickness, increased porosity and decreased estrogen receptor alpha in bone in adult 129/Sv female mice
Authors: Artsi Hanna , Cohen-Kfir Einav , Shahar Ron , Kalish-Achrai Noga , Lishinsky Natan , Dresner-Pollak Rivka 
Published: December 2022

IntroductionSirtuin 1 (SIRT1) is a key player in aging and metabolism and regulates bone mass and architecture. Sexual dimorphism in skeletal effects of SIRT1 has been reported, with an unfavorable phenotype primarily in female mice.MethodsTo investigate the mechanisms of gender differences in SIRT1 skeletal effect, we investigated femoral and vertebral cortical and cancellous bone in global Sirt1 haplo-insufficient 129/Sv mice aged 2,7,12 months lacking Sirt1 exons 5,6,7 (Sirt1+/Δ) and their wild type (WT) counterparts.ResultsIn females, femoral bone mineral content, peak cortical thickness, and trabecular bone volume (BV/TV%), number and thickness were significantly lower in Sirt1+/Δ compared to WT mice. Increased femoral cortical porosity was observed in 7-month-old Sirt1+/Δ compared to WT female mice, accompanied by reduced biomechanical strength. No difference in vertebral indices was detected between Sirt1+/Δ and WT female mice. SIRT1 decreased with aging in WT female mice and was lower in vertebrae and femur in 18- and 30- versus 3-month-old 129/Sv and C57BL/6J female mice, respectively. Decreased bone estrogen receptor alpha (ERα) was observed in Sirt1+/Δ compared to WT female mice and was significantly higher in Sirt1 over-expressing C3HT101/2 murine mesenchymal stem cells. In males no difference in femoral indices was detected in Sirt1+/Δ versus WT mice, however vertebral BV/TV%, trabecular number and thickness were higher in Sirt1+/Δ vs. WT mice. No difference in androgen receptor (AR) was detected in bone in Sirt1+/Δ vs. WT male mice. Bone SIRT1 was significantly lower in male compared to female WT mice, suggesting that SIRT1 maybe more significant in female than male skeleton.DiscussionThese findings demonstrate that 50% reduction in SIRT1 is sufficient to induce the hallmarks of skeletal aging namely, decreased cortical thickness and increased porosity in female mice, highlighting the role of SIRT1 as a regulator of cortical bone quantity and quality. The effects of SIRT1 in cortical bone are likely mediated in part by its regulation of ERα. The age-associated decline in bone SIRT1 positions SIRT1 as a potential therapeutic target to ameliorate age-related cortical bone deterioration in females. The crosstalk between ERα, AR and SIRT1 in the bone microenvironment remains to be further investigated.
Bioinformatics analysis identifies immune-related gene signatures and subtypes in diabetic nephropathy
Authors: Lu Kunna , Wang Li , Fu Yan , Li Guanghong , Zhang Xinhuan , Cao Mingfeng 
Published: December 2022

BackgroundSystemic inflammation and immune response are involved in the pathogenesis of diabetic nephropathy (DN). However, the specific immune-associated signature during DN development is unclear. Our study aimed to reveal the roles of immune-related genes during DN progression.MethodsThe GSE30529 and GSE30528 datasets were acquired from the Gene Expression Omnibus (GEO) database. Then, the intersection between differentially expressed genes (DEGs) and immune score-related genes (ISRGs) was screened. Subsequently, functional enrichment analyses were performed. The different immune phenotype-related subgroups were finally divided using unsupervised clustering. The core genes were identified by WGCNA and the protein-protein interaction (PPI) network. xCell algorithm was applied to assess the proportion of immune cell infiltration.Results92 immune score-related DEGs (ISRDEGs) were identified, and these genes were enriched in inflammation- and immune-associated pathways. Furthermore, two distinct immune-associated subgroups (C1 and C2) were identified, and the C1 subgroup exhibited activated immune pathways and a higher percentage of immune cells compared to the C2 subgroup. Two core genes (LCK and HCK) were identified and all up-regulated in DN, and the expressions were verified using GSE30122, GSE142025, and GSE104954 datasets. GSEA indicated the core genes were mainly enriched in immune-related pathways. Correlation analysis indicated LCK and HCK expressions were positively correlated with aDC, CD4+ Tem, CD8+T cells, CD8+ Tem, and mast cells.ConclusionsWe identified two immune-related genes and two immune-associated subgroups, which might help to design more precise tailored immunotherapy for DN patients.
Techno-economic assessment of jet fuel production using the Fischer-Tropsch process from steel mill gas
Authors: Collis Jason , Duch Karsten , Schomäcker Reinhard 
Published: December 2022

In order to reduce human-made global warming, the aviation industry is under pressure to reduce greenhouse gas (GHG) emissions. Production of sustainable aviation fuel (SAF) from steel mill gases could help reduce the emissions intensity of jet fuel. This study presents a simulation, techno-economic assessment, and GHG emissions assessment of a Fischer-Tropsch (FT) process using two steel mill gases (coke oven gas and blast furnace gas) as feedstock. The process was analysed both with and without carbon capture and storage (CCS) to reduce process emissions. The minimum viable selling price (MVSP) was determined to be 1,046 €/tonne for the standard scenario and 1,150 €/tonne for the CCS scenario, which is higher than the fossil-fuel-based benchmark (325–1,087 €/tonne since 2020), although similar to the lowest costs found for other SAF benchmarks. The GHG emissions intensity was found to be 49 gCO2-eq./MJ for the standard scenario and 21 gCO2-eq./MJ with CCS, far lower than the 88 gCO2-eq./MJ average for the conventional benchmark and in the mid-lower range of found emissions intensities for other SAF benchmarks. When a CO2 tax of 130 €/tonne is considered, the MVSP for the standard scenario increases to 1,320 €/tonne while the CCS scenario increases to 1,269 €/tonne, making them cost-competitive with the fossil-fuel benchmark (797–1,604 €/tonne). The studied process offers economically viable small-to-medium scale SAF plants (up to 50 kt/y SAF) at a CO2 tax of 190 €/tonne or higher for the CCS scenario and 290 €/tonne or higher for the standard scenario.
Characteristics of piRNAs and their comparative profiling in testes of sheep with different fertility
Authors: Di Ran , Zhang Rensen , Mwacharo Joram Mwashigadi , Wang Xiangyu , He Xiaoyun , Liu Yufang , Zhang Jinlong , Gong Yiming , Zhang Xiaosheng , Chu Mingxing 
Published: December 2022

As a novel class of small RNAs, piRNAs are highly expressed in the animal gonads and their main known role is to inhibit transposon activity for ensuring the correctness and integrity of genome. In order to explore the characteristics of piRNAs in sheep testis and their possible regulatory roles on male reproduction, deep sequencing technology was used to sequence small RNAs and identify piRNAs in testes of sheep. The length of piRNAs in sheep testes showed a unimodal distribution between 26 and 31 nt, with a peak at 29 nt. These piRNAs exhibited obvious ping-pong signature and strand specificity. In the genome, they were mainly aligned to CDS, intron, repetitive sequence regions and unannotated regions. Furthermore, in transposon analysis, piRNAs were aligned predominantly to LINE, SINE, and LTR types of retrotransposon in sheep testes, and the piRNAs derived from each type showed obvious ping-pong signature. The piRNA clusters identified in sheep testes were mainly distributed on chromosomes 3, 7, 15, 17, 18 and 20. The results combining semen determination with pathway enrichment analysis implied that differentially expressed piRNAs between the testes of rams with different fertility might participate in spermatogenesis by regulating multiple pathways closely related to stabilization of blood-testis barrier and renewal and differentiation of spermatogonial stem cell. Taken together, the study provided new insights into the characteristics, origin and expression patterns of piRNAs in sheep testes tissue, which would help us better understand the role of piRNAs in sheep reproduction.
Different local, innate and adaptive immune responses are induced by two commercial Mycoplasma hyopneumoniae bacterins and an adjuvant alone
Authors: Beuckelaere Lisa , Haspeslagh Maarten , Biebaut Evelien , Boyen Filip , Haesebrouck Freddy , Krejci Roman , Meyer Evelyne , Gleerup David , De Spiegelaere Ward , Devriendt Bert , Maes Dominiek 
Published: December 2022

IntroductionEnzootic pneumonia still causes major economic losses to the intensive pig production. Vaccination against its primary pathogen, Mycoplasma hyopneumoniae, is carried out worldwide to control the disease and minimize clinical signs and performance losses. Nonetheless, the effects of both infection with, and vaccination against Mycoplasma hyopneumoniae on the innate and adaptive immune responses remain largely unknown. Therefore, we conducted a study in which piglets were injected once with a commercial bacterin V1 or V2, or the adjuvant of V1 (A) to investigate their effect on local, innate and adaptive immune responses.MethodsThree weeks after vaccination, piglets were challenge infected with M. hyopneumoniae and euthanized four weeks later to assess vaccine efficacy via macroscopic and microscopic evaluation of lung lesions. Blood and broncho-alveolar lavage fluid (BAL) samples were collected to measure antibody responses, cellular immunity, BAL cytokine levels and BAL M. hyopneumoniae DNA load as well as cytokine secretion by monocytes.ResultsAfter vaccination, proliferation of antigen-specific CD3+ T cells and a higher percentage of TNF-α+ CD8+, and TNF-α+ and TNF-α+IFN-γ+ CD4+CD8+ T cells was seen in V1, while proliferation of or a significant increase in cytokine production by different T cell subsets could not be observed for animals from V2. Interestingly, LPS-stimulated blood monocytes from V1 and A secreted less IL-10 on D7. After challenge, higher levels of IgA, more IL-10 and less IL-1β was detected in BAL from V1, which was not observed in V2. Animals from A had significantly more IL-17A in BAL. The macroscopic lung lesion score and the M. hyopneumoniae DNA load at euthanasia was lower in V1, but the microscopic lung lesion score was lower in both vaccinated groups.DiscussionIn conclusion, these results indicate that the two commercial bacterins induced different local and adaptive immune responses, that the adjuvant alone can reduce anti-inflammatory innate immune responses, and that both vaccines had a different efficacy to reduce Mycoplasma-like lung lesions and M. hyopneumoniae DNA load in the lung.
Cooperation between cGAS and RIG-I sensing pathways enables improved innate recognition of HIV-1 by myeloid dendritic cells in elite controllers
Authors: Martin-Gayo Enrique , Gao Ce , Calvet-Mirabent Marta , Ouyang Zhengyu , Lichterfeld Mathias , Yu Xu G. 
Published: December 2022

IntroductionSpontaneous control of HIV-1 replication in the absence of anti-retroviral therapy (ART) naturally occurs in a small proportion of HIV-1-infected individuals known as elite controllers (EC), likely as a result of improved innate and adaptive immune mechanisms. Previous studies suggest that enhanced cytosolic immune recognition of HIV-1 reverse transcripts in conventional dendritic cells (mDC) from EC enables effective induction of antiviral effector T cell responses. However, the specific molecular circuits responsible for such improved innate recognition of HIV-1 in mDC from these individuals remain unknown.Results and methodsHere, we identified a subpopulation of EC whose mDC displayed higher baseline abilities to respond to intracellular HIV-1 dsDNA stimulation. A computational analysis of transcriptional signatures from such high responder EC, combined with functional studies, suggested cytosolic recognition of HIV-1 dsDNA by cGAS, combined with sensing of viral mRNA by RIG-I after polymerase III-mediated HIV-1 DNA transcription.DiscussionTogether, our work identifies collaborative networks of innate sensing pathways that enhance cell-intrinsic abilities of mDC to induce antiviral innate responses against HIV-1; these observations might be useful for the therapeutic induction of effective antiviral immune responses.