bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reliability of Shear Wave Elastography for the Assessment of Gastrocnemius Fascia Elasticity in Healthy Individual
Authors: Zhou Jiping , Lin Yuyi , Zhang Jiehong , Si’tu Xingxian , Wang Ji , Pan Weiyi , Wang Yulong 
Published: January 2022

Abstract
The mechanical properties of deep fascia (i.e. an index of stiffness) strongly affect the development of muscle pathologies, and muscular actions, such as compartment syndromes. Actually, a clear understanding of the mechanical characterization of muscle deep fascia still lacks. The present study focuses on examining the reliability of ultrasonic shear wave elastography device (USWE) in quantifying the shear modulus of gastrocnemius fascia in healthy individual and the device’s abilities to examine the shear modulus of gastrocnemius deep fascia during ankle dorsiflexion. Twenty-one healthy males participated in the study (age: 21.48±1.17 years). The shear modulus of the medial gastrocnemius fascia (MGF) and lateral gastrocnemius fascia (LGF) were quantified at different angles using USWE during passive lengthening. The operators took turns to measure each subject’s MGF and LGF over 1-hour period and by operator B with a 2-hour interval. In the intra-operator test, the same subjects participated at the same time 5 days later. The intra-rater [ Intra-class correlation coefficient (ICC) = 0.846-0.965)] and inter-rater (ICC = 0.877-0.961) reliabilities for measuring the shear modulus of the MGF and LGF were rated as both excellent, and the standard error in measurement (SEM) was 3.49 kPa, the minimal detectable change (MDC) was 9.68 kPa. Regardless of the ankle angle, the shear modulus of the LGF were significant greater than that of the MGF (p < 0.001). The significant increase in the shear modulus both of the MGF and LGF were observed at neutral position compared to the relaxed position. This results indicate that the USWE is a technique to assess the shear modulus of gastrocnemius fascia and detect its dynamic changes during ankle dorsiflexion. USWE can be used for biomechanical study and intervention experiments of deep fascia.
Apigenin Induces Browning in White Adipocytes Mediated by VEGF-PRDM16 Signaling.
Authors: Sreeku Sreelekshmi , Vijayan Vinu , Singh Fathe , Sudhakar Manu , S Kiran M 
Published: January 2022

Abstract
The white adipose tissues are metabolically inert which results in deranged biological signalling disorders resulting in obesity. Lack of vascularisation in these tissues is mainly responsible to make them metabolically inert. Not much work has been done in this direction to understand the role of angiogenesis in white adipocytes metabolism. In the present study, we evaluated the effect of angiogenic modulator in modulating the metabolism in white adipocyte. Nutraceuticals apigenin (Apg) was employed as angiogenic modulator. The results indicated that promoting angiogenesis by Apg enhanced the de novo differentiation and trans-differentiation of white adipocyte into brown like phenotype by triggering vascular endothelial growth factor A. Cross talk between endothelial and adipocytes were observed in co-culture studies. The metabolic shift in white adipocytes was observed to be due to the upregulation of PRDM16 cascade. The study provides new insights for inducing metabolic shift in white adipocytes by modulation of angiogenesis in white adipocyte to trigger browning for the treatment of obesity. Further the study opens scopes for development of medical devices for obese subjects, an area that needs to be addressed specifically with reference to soft tissue engineering as commercial soft tissue engineering scaffolds does not suit the obese patients.
The Diagnostic and Prognostic Value of BEN-Domain (BEND) Family Genes in Gastric Cancer
Authors: Fan Jiaxin , Yang Min , Liang Chaojie , Liang Chaowei , Guo Jiansheng 
Published: January 2022

Abstract
BEND(BEN domain-containing protein)is a domain protein-coding gene, whose abnormal expression is related to the occurrence of malignant tumors. But studies on gastric cancer are rare. We attempted to investigate the role of BEND family genes in evaluating the prognosis of gastric cancer and guiding clinical treatment. We analyzed the BEND family genes expression, prognostic value, and drug sensitivity in pan-cancer, and the correlation between their expression and tumor microenvironment of gastric cancer, stemness index, immune subtypes, and clinicopathological characteristics were analyzed. We constructed a model using BEND3P1 and BEND6 to evaluate the prognosis of gastric cancer patients. Multivariate Cox proportional risk model analysis showed that risk score is an independent risk factor for gastric cancer patients. To assess the value of risk score for prognosis, patients were divided into high-risk and low-risk groups based on median risk scores, and survival analyses were performed. The results showed that the OS of patients with high-risk scores is significantly lower. We also constructed a nomogram to predict individual survival probability using the BEND risk score and clinical case characteristics. In conclusion, the BEND family genes can predict the prognosis and guide the treatment of gastric cancer patients.
Bmi1 Promotes Cholangiocarcinoma Progression And Correlates With Antitumor Immunity In An Exosome-Dependent Manner
Authors: Liu Zengli , Hu Chunxiao , Zheng Lijie , Liu Jialiang , Li Kangshuai , Li Xingyong , Wang Yue , Mu Wentao , Chen Tianli , Shi Anda , Qiu Bo , Zhang Xin , Zhang Zongli 
Published: January 2022

Abstract
Background: Cholangiocarcinoma (CCA) is a class of malignant tumors originating from bile duct epithelial cells. Due to difficult early diagnosis and limited treatment, the prognosis of CCA is extremely poor. Bmi1 is dysregulated in many human malignancies. However, the prognostic significance and oncogenic role of Bmi1 in cholangiocarcinoma (CCA) are not well elucidated. Methods: In the present study, we investigated its clinical importance and the potential mechanisms in the progression of CCA. We detected Bmi1 expression in a large CCA cohort. We demonstrated Bmi1 was substantially upregulated in CCA tissues and was identified as an independent prognostic biomarker of CCA. Moreover, overexpression of Bmi1 promoted CCA proliferation, migration, and invasion. And, Bmi1 knockdown could inhibit proliferation and metastases of CCA in vitro and in vitro/vivo validation. Interestingly, we found that CCA-derived exosomes contain Bmi1 proteins, which can transfer Bmi1 between CCA cells. The unique Bmi1-containing exosomes promote CCA proliferation and metastasis through autocrine/paracrine mechanisms. In addition, we demonstrated that Bmi1 inhibits CD8+T cell-recruiting chemokines by promoting repressive H2A ubiquitination in CCA cells. Conclusions: Bmi1 is an unfavorable prognostic biomarker of CCA. Our data demonstrate a novel function of Bmi1 in CCA tumorigenesis and metastasis mediated by exosomes. Besides, Bmi1 inhibition may augment immune checkpoint blockade to inhibit tumor progression by activating cell-intrinsic immunity of CCA
Psychometric Properties of the Emotional Processing Scale in Individuals with Psychiatric Symptoms and the Development of a Brief 15-Item Version
Authors: Maroti Daniel , Axelsson Erland , Ljótsson Brjánn , Andersson Gerhard , Lumley Mark , Johansson Robert 
Published: January 2022

Abstract
Objective

The 25-item Emotional Processing Scale (EPS) is a measure of emotional processing. There is a scarcity of research about its factor structure, test-retest reliability, and validity in individuals with psychiatric symptoms.
Method

We administered the EPS-25 to a sample (N=512) of people with elevated psychiatric symptoms, using confirmatory factor analysis to evaluate three a priori models from previous research. We then evaluated discriminant and convergent validity against measures of alexithymia (Toronto Alexithymia Scale-20; TAS-20), depressive symptoms (Patient Health Questionaire-9; PHQ-9) and anxiety symptoms (Generalized Anxiety Disorder-7; GAD-7).
Results

None of the a priori models achieved acceptable fit, and subsequent exploratory factor analysis did not lead to a convincing factor solution for the 25 items. A 5-factor model did, however, achieve acceptable fit if we retained only 15 items (EPS-15) and this solution could be replicated in a holdout sample. Convergent and discriminant validity for EPS-15 was r=-.19-.46 vs. TAS-20, r=.07-.25 vs. PHQ-9, r=.29-.57 vs. GAD-7. Test-retest reliability was ICC=.73.
Conclusions

This study strengthens the case for the reliability and validity of the five factor Emotional Processing Scale but suggest that only 15 items should be retained. Future studies should further examine the reliability and validity of the EPS-15.
Industry-Oriented Method for Dynamic Force Identification in Peripheral Milling Based on FSC-LSQR Using Acceleration Signals
Authors: Hou Maxiao , Cao Hongrui , Li Qi , Shi Jianghai 
Published: January 2022

Abstract
Online measurement of milling force play a vital role in enabling machining process monitoring and control. In practice, the milling force is difficult to be measured directly with the dynamometer. This paper develops a novel method for milling force identification called least square QR-factorization with fast stopping criterion (FSC-LSQR) method, and the queue buffer structure (QBS) is employed for the online identification of milling force using acceleration signals. The convolution integral of milling force and acceleration signals is discretized, which turns the problem of milling force identification into a linear discrete ill-posed problem. The FSC-LSQR algorithm is adopted for milling force identification because of its high efficiency and accuracy, which handles the linear discrete ill-posed problem effectively. The online identification of milling force can be realized using the acceleration signal enqueue and the milling force dequeue operations of the QBS. Finally, the effectiveness of the method is verified by experiments. The experimental results show that the FSC-LSQR algorithm running time is within \((0.05s)\) and the calculation error is less than \((10\%)\). The proposed method can make the sampling frequency of the milling force reach 10240Hz by employing QBS, which satisfy the industry requirements of milling force measurement.
Dexmedetomidine Versus Propofol Sedation in Flexible Bronchoscopy: A Randomized Controlled Trial and a Systematic Review and Meta-Analysis
Authors: Pertzov Barak , Krasulya Boris , Azem Karam , Shostak Yael , Izhakian Shimon , Rosengarten Dror , Khaershenko Svetlana , Kramer Mordechai Reuven 
Published: January 2022

Abstract
Background:Dexmedetomidine (DEX), is a highly selective alpha2 adrenoceptor (α2-AR) agonist, successfully used in various procedures including flexible bronchoscopy. Randomized controlled trials (RCTs) evaluating DEX sedation during bronchoscopy report equivocal results regarding respiratory and hemodynamic outcomes. Methods We conducted an RCT to evaluate the efficacy of dexmedetomidine compared to propofol for sedation during bronchoscopy. The primary outcome was desaturation events, secondary outcomes were transcutaneous Pco2 level, hemodynamic adverse events and physician and patient satisfaction. We have also conducted A systematic review and meta-analysis of all RCTs evaluating DEX sedation during flexible bronchoscopy, included current study results.ResultsOverall, 63 patients were included, 30 and 33 in the DEX and propofol groups, respectively. The number of desaturation events was similar between groups, median (IQR) 1 (0-1) and 1 (0-2) in the DEX and control groups, respectively (P=0.29). Median desaturation time was 1 (0-2) and 1 (0-3) minutes in the DEX and control groups, respectively (P=0.48). Adverse events included hypotension, 33% vs 21.1% in intervention and control groups, respectively (P=0.04), bradycardia, cough, and delayed recovery from sedation. Total adverse events were 22 and 7 in DEX and propofol groups, respectively (P=0.009). The pooled meta-analysis included 13 trials (1604 participants) showed a significantly lower rate of desaturation events in the DEX group (RR 0.67, 95% CI 0.57 to 0.79) with a significantly higher rate of hypotension and bradycardia events (RR 1.55, 95% CI 1.16 to 2.06 and RR 1.91, 95% CI 1.04 to 3.5, respectively)ConclusionDexmedetomidine sedation resulted in a significantly reduced rate of desaturation events in comparison to propofol, midazolam and fentanyl. However, it was also associated with a higher rate of hypotension and bradycardia.Trial registration : NCT04211298, registration date: 26/12/2019
Farm Parcel Extraction in High Resolution Remote Sensing Image Based on Hierarchical Spectrum and Shape Features
Authors: Li Bangyu 
Published: January 2022

Abstract
Background: Land-use classification schemes typically address both land use and land cover. Vectorized data extracted from farm parcel segmentation provides important cadastral data for the formulation and management of climate change policies. It also provides important basic data for research on pest control in large areas, crop yield forecasts, and crop varieties classification. It can also be used for the assessment of compensation for damages related to extreme weather events by the agricultural insurance department. Firstly, we investigate the effectiveness of an automated image segmentation method based on TransUNet architecture to enable that automate the task of farm parcel delineation that originally relied on high labor costs. Then, post-processing by vectoring binary segmentation image, which the area and regularity parameter to adjust the accuracy of segmentation, can get a more optimized image segmentation result.Results: The results on the existing data show that the automatic segmentation system we proposed is a method that can effectively divide various types of agricultural land. The system was trained and evaluated using 94780 images. The performance parameters obtained showed that the accuracy rate reached 83.31%, the recall rate reached 82.13%, the F1-S rate was 80.37%, the total accuracy rate was 82.23%, and Iou was 80.39%. At the same times, without losing too much accuracy, we train and test the model with 3m resolution image, which has the advantage of processing speed than 0.8m resolution. Therefore, our proposed method can be effectively applied to the task of extraction of agricultural land, which is better and more efficient than most manual annotations.Conclusions: We have demonstrated the effectiveness of strategy using a TransUNet architecture and postprocessing by vectoring binary segmentation for farm parcel extraction in high remote sensing images. The success of our approach is also a demonstration of feasibility of the deep learning to participate in and improve agricultural production activities, which is important for achieving scientific management of agricultural production.
Phenylalanine Suppresses Cell Death Caused by Loss of Fumarylacetoacetate Hydrolase in Arabidopsis
Authors: Jiang Yihe , Zhu Qi , Yang Hua , Zhi Tiantian , Ren Chunmei 
Published: January 2022

Abstract
Fumarylacetoacetate hydrolase (FAH) catalyzes the final step of Tyrosine (Tyr) degradation pathway essential to animals and the deficiency of FAH causes an inborn lethal disease. In plants, a role of this pathway was unknown until we found that mutation of Short-day Sensitive Cell Death1 (SSCD1), encoding Arabidopsis FAH, results in cell death under short day. Phenylalanine (Phe) could be converted to Tyr and then degraded in both animals and plants. Phe ingestion in animals worsens the disease caused by FAH defect. However, in this study we found that Phe represses cell death caused by FAH defect in plants. Phe treatment promoted chlorophyll biosynthesis and suppressed the up‑regulation of reactive oxygen species marker genes in the sscd1 mutant. Furthermore, the repression of sscd1 cell death by Phe could be reduced by α-aminooxi-β-phenylpropionic acid but increased by methyl jasmonate, which inhibits or activates Phe ammonia-lyase catalyzing the first step of phenylpropanoid pathway, respectively. In addition, we found that jasmonate signaling up‑regulates Phe ammonia-lyase 1 and mediates the methyl jasmonate enhanced repression of sscd1 cell death by Phe. These results uncovered the relation between chlorophyll biosynthesis, phenylpropanoid pathway and jasmonate signaling in regulating the cell death resulting from loss of FAH in plants.
Gene expression and DNA methylation altering lead to the high oil content in wild allotetraploid peanut (A. monticola)
Authors: Liu Nian , Pandey Manish , Wu Bei , Huang Li , Luo Huaiyong , Chen Yuning , Zhou Xiaojing , Chen Weigang , Huai Dongxin , Lei Yong , Liao Boshou , Varshney Rajeev , Jiang Huifang 
Published: January 2022

Abstract
The wild allotetraploid peanut Arachis monticola contains higher oil content than cultivated allotetraploid Arachis hypogaea. To investigate its molecular mechanism controlling oil accumulation, we performed comparative transcriptomics from developing seeds between three Arachis monticola and five Arachis hypogaea varieties. The analysis not only showed species-specific grouping based on transcriptional profiles but also detected two gene clusters with divergent expression patterns enriched in lipid metabolism. Further, the differential expression gene analysis also indicated expression alteration in wild peanut leading to enhanced activity of oil biogenesis and limiting the rate of lipid degradation. We also constructed a regulatory network of lipid metabolic DEGs with co-expressed transcription factors. In addition, bisulfite sequencing was conducted to characterize the variation of DNA methylation between wild allotetraploid (245, WH 10025) and cultivated allotetraploid (Z16, Zhh 7720) genotypes. Genome-wide DNA methylation was found antagonistically correlated with gene expression during seed development. The results indicated that CG and CHG contexts methylation may negatively regulate specific lipid metabolic genes and transcription factors to subtly affect the difference of oil accumulation. Our work provided the first glimpse on the regulatory mechanism of gene expression altering for oil accumulation in wild peanut and gene resources for future breeding applications.
A Novel Approach of Tracing in Vivo Bioluminescence Imaging Expression of Vitrified Immature Testicular Tissue Grafts Until Adulthood: A Translational Transgenic Mouse Model
Authors: Lu Buo-Jia , Liu Yung-Liang , Lin Bou-Zenn , Chen Chi-Huang 
Published: January 2022

Abstract
Background: The optimal method for cryopreserving immature testicular tissue (ITT) remains unknown and there is no standardized protocol. Controlled slow freezing remains the mainstream method of choice in human prepubertal male fertility preservation. Currently, the outcomes for ITT vitrification are conflicting, and most data are limited to in vitro animal studies.Methods: A total of 12 pairs of donor and recipient mice were included in our experiments. The donors were immature transgenic mice, and the recipients were wild-type male mice. In the vitrification group, ITT was vitrified and thawed before transplantation. In the control group, ITT was transplanted to the recipients immediately. After thawing, we measured the expression of apoptosis-related mRNA caspase-3. More importantly, we monitored to adulthood all the transplanted grafts in vivo using noninvasive bioluminescence imaging (BLI) technology. On day 31, we removed the grafts for evaluation via hematoxylin and eosin staining and immunohistochemistry (IHC).Results: We traced the survival of the grafts by in vivo BLI on days 1, 2, 5, 7, and 31 after transplantation. In both the vitrification and the control groups, bioluminescence decreased between days 2 and 5. Subsequently, the bioluminescence showed an upward trend until day 31. Compared with day 1, the bioluminescence was significantly stronger on day 31 after transplantation (P = 0.009). The differences between the two groups were constantly insignificant after analysis. These results indicate that both fresh and frozen–thawed testicular tissues can survive for at least 31 days after transplantation. Moreover, the vitrification group showed BLI signals comparable with those of fresh tissues. Compared with the control group, expression of the caspase-3 gene was significantly increased after vitrification (P = 0.04). Histology and IHC showed that both tissue structure and protein expression were intact in both groups.Conclusions: Transplanted vitrified ITT grafts could survive till adulthood with BLI intensity comparable to that of the fresh control. Intact cells and structures for spermatogenesis in vitrified ITT grafts were as well-preserved as those in the control group. This translational model of self-repairing vitrified ITT grafts in vivo, lends weight to the role of vitrification in prepubertal male fertility preservation.
Optimal Solution of Neutrosophic Linear Fractional Programming Problems With Mixed Constraints
Authors: DAS SAPAN , Edalatpanah S A 
Published: January 2022

Abstract
In this paper, Linear Fractional Programming (LFP) problems have been extended to neutrosophic sets (NSs) and the operations and functionality of these laws are studied. Moreover, the new algorithm is based on aggregation ranking function and arithmetic operations of triangular neutrosophic sets (TNSs). Furthermore, for the first time, in this paper, we take up a problem where the constraints are both equality and inequality neutrosophic triangular fuzzy number. Lead from genuine issue, a few numerical models are considered to survey the legitimacy, profitability and materialness of our technique. At last, some numerical trials alongside one contextual analysis are given to show the novel techniques are better than the current strategies.