bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract B46: Immune characterization of C57BL/6J syngeneic breast cancer mouse models for application in immunotherapy development
Authors: Magna Melinda , Lippman Marc E , Hudson Barry I 
Published: December 2022

Abstract
Background: Breast cancer is the second leading cause of cancer-related mortality in women worldwide. While immunotherapy has shown success in various solid tumors, it has been largely ineffective in breast cancer. Critical to establishing effective immunotherapies in breast cancer is the development and better characterization of the tumor immunology of immunocompetent syngeneic models. Here, we describe the molecular and in-depth immunological characterization of three C57BL/6J syngeneic breast cancer mouse models: AT3, Py8119 and E0771 cells. Methods: AT3, Py8119, and E0771 cells were obtained from commercial sources. 5x105 cells were implanted orthotopically in 8 weeks old C57BL/6J female mice. Tumors were isolated from mice and breast cancer subtype classification was performed by immunohistochemical (IHC) staining of estrogen receptor alpha (ERα), progesterone receptor (PR) and Erb-B2 Receptor Tyrosine Kinase 2 (ERBB2/HER2). T cell and myeloid cell infiltration patterns of the tumors were determined by CD3 and CD11b IHC staining respectively. Immunophenotyping of splenic immune cell populations and tumor-infiltrating immune cells was performed with multicolor flow cytometry in all three models. Results: Based on the IHC analysis of the orthotopic murine tumors, E0771 and Py8119 tumors fit the histopathological criteria of triple-negative breast cancer models, while AT3 tumors appear to be a model of the HER2-enriched breast cancer subtype. We show significant differences between the AT3, Py8119 and E0771 models regarding the accumulation of myeloid cells (mMDSC, gMDSC, macrophages, dendritic cells) in the tumor and spleen. Furthermore, analysis of tumor-infiltrating CD4 and CD8 T lymphocytes showed high expression levels of PD-1 in all three models, and varying levels of expression of other immune checkpoint molecules (CTLA4, TIM3, LAG3 and PD-L1) that were model specific. Furthermore, flow cytometry analysis also revealed high levels of PD-L1 expression on all three tumor cell types. Conclusion: In summary, we determined the breast cancer molecular subtype, and described the splenic and intratumoral immune landscape of the AT3, Py8119 and E0771 orthotopic C57BL/6J syngeneic breast cancer mouse models. These data will aid the better design of future tumor immunological studies and provide essential knowledge about these preclinical breast cancer models for use in immunotherapy development.
Citation Format: Melinda Magna, Marc E Lippman, Barry I Hudson. Immune characterization of C57BL/6J syngeneic breast cancer mouse models for application in immunotherapy development [abstract]. In: Proceedings of the AACR Special Conference: Tumor Immunology and Immunotherapy; 2022 Oct 21-24; Boston, MA. Philadelphia (PA): AACR; Cancer Immunol Res 2022;10(12 Suppl):Abstract nr B46.
Abstract IA11: Immune selection and predictability of cancer evolution
Authors: Luksza Marta 
Published: December 2022

Abstract
Immune editing of neoantigens is crucial for the success of immunotherapies, but it is still unknown to what extent the immune system naturally edits evolving tumors and what is the fitness cost associated with the presence of neoantigens. Here we develop a biophysically grounded neoantigen quality model, which quantifies the immunogenicity of tumor neoantigens. We use the model to define the fitness of tumor clones as a combination of negative selection due to immune recognition and positive selection due to oncogenic mutations. We investigate how pancreatic cancers – a lowly mutated, poorly immunogenic cancer, largely presumed to not be subject to immunoediting – evolve over 10 years. Our patient cohort includes a set of long-term survivors, who are characterized by high levels of immune infiltration of their primary tumors. With the fitness model, we show that long-term survivors evolve new clones of markedly lower immune fitness cost, to indicate clones with high-quality neoantigens are negatively selected. Importantly, the fitness model predicts the clonal composition of recurrent tumors of the patients. Thus, we submit longitudinal evidence that the human immune system naturally edits neoantigens. Furthermore, we present a model that describes how tumor cell populations evolve under immune pressure over time, with implications for cancer biology and therapy.
Citation Format: Marta Luksza. Immune selection and predictability of cancer evolution [abstract]. In: Proceedings of the AACR Special Conference: Tumor Immunology and Immunotherapy; 2022 Oct 21-24; Boston, MA. Philadelphia (PA): AACR; Cancer Immunol Res 2022;10(12 Suppl):Abstract nr IA11.
Buddhist Homiletics on Gambling (*Saddharmaparikathā, Ch. 12)
Authors: Szántó Péter-Dániel 
Published: December 2022

Abstract
The paper focuses on the 12th chapter of the *Saddharmaparikathā, a Buddhist homileticians’ guidebook containing sample sermons, dealing with the topic of gambling (dyūta). I edit, translate, and discuss the chapter with an introduction that includes a short overview of gambling in Sanskrit literature at large. The anonymous author is dismissive of gambling in all its forms, whether it is practised for material gain, for mere pleasure, and even if studied as an art. In spite of its exiguity, his discussion of the topic is, as far as we are aware, the most comprehensive in classical Buddhist literature.
Interaction between marine protists and bacteria results in magnetotaxis and iron recycling
Authors: Mandal Fatik Baran 
Published: December 2022

Abstract
Marine protists are eukaryotic trophic linkers that play a crucial role in iron recycling. Some marine protists have the ability of magnetotaxis, which they gain by consuming their ectosymbiotic bacteria. They graze and internalize the magnetotactic bacteria along with their magnetosome chains. Through egestion, marine protists avoid iron toxicity. Colloidal iron digestion by protists produces bioavailable iron for other marine organisms, passing to phytoplankton and mesozooplankton through the mesotrophic system. Indeed, ectosymbiotic bacteria and their protistan host form a microbial holobiont acting as an ecological unit. Some of the genetic mechanisms influencing the biosynthesis of magnetite in both prokaryotes and eukaryotes appear to be common. The recorded history of the magnetoreception ability of some marine protists goes back to the study by F.F. Torres de Araujo in 1986. After research over 35 years or more, it is safe to record that magnetotaxis in marine protists is yet to be fully understood, and might be similar to that of free-living magnetotactic bacteria. However, the attainment of magnetotaxis by protistan grazers through bacterivory and its role in iron recycling in the marine ecosystem is very interesting. The present article aims to provide an account of such interesting facts.
Creatio ex Creatione a Natura Amoris
Authors: Orr Brian J. 
Published: December 2022

Abstract
Creatio ex nihilo is the dominant creation theory in the Christian tradition. Relational theologian Thomas Jay Oord proposes an alternative creation theory, in which God everlastingly creates from what he previously created. Key reasons why Oord argues creatio ex nihilo should be rejected are that it has Gnostic roots, is not explicitly taught in Scripture, and is illogical. This article critically assesses Oord’s arguments against creatio ex nihilo, contending that his conclusions are misguided due to a Biblicist tendency in his reading of the text and the inaccurate definition of creatio ex nihilo he argues against. With the biblical and historical data, a definition of creatio ex nihilo representative of the Christian tradition is articulated. This essay then demonstrates how creatio ex nihilo is superior to Oord’s alternative theory.
Monte Carlo Simulations for Evaluating the Accuracy of Geostationary Lightning Mapper Detection Efficiency and False Alarm Rate Retrievals
Authors: Virts Katrina S. , Koshak William J. 
Published: December 2022

Abstract
Performance assessments of the Geostationary Lightning Mapper (GLM) are conducted via comparisons with independent observations from both satellite-based sensors and ground-based lightning detection (reference) networks. A key limitation of this evaluation is that the performance of the reference networks is both imperfect and imperfectly known, such that the true performance of GLM can only be estimated. Key GLM performance metrics such as detection efficiency (DE) and false alarm rate (FAR) retrieved through comparison with reference networks are affected by those networks’ own DE, FAR, and spatiotemporal accuracy, as well as the flash matching criteria applied in the analysis. This study presents a Monte Carlo simulation–based inversion technique that is used to quantify how accurately the reference networks can assess GLM performance, as well as suggest the optimal matching criteria for estimating GLM performance. This is accomplished by running simulations that clarify the specific effect of reference network quality (i.e., DE, FAR, spatiotemporal accuracy, and the geographical patterns of these attributes) on the retrieved GLM performance metrics. Baseline reference network statistics are derived from the Earth Networks Global Lightning Network (ENGLN) and the Global Lightning Dataset (GLD360). Geographic simulations indicate that the retrieved GLM DE is underestimated, with absolute errors ranging from 11% to 32%, while the retrieved GLM FAR is overestimated, with absolute errors of approximately 16% to 44%. GLM performance is most severely underestimated in the South Pacific. These results help quantify and bound the actual performance of GLM and the attendant uncertainties when comparing GLM to imperfect reference networks.
Rituximab-induced severe acute thrombocytopenia in a patient with splenic marginal zone lymphoma
Authors: Kars Taha Ulutan , Yorgancı Zahit Furkan , Yaşkıran Osman , Tekinalp Atakan , Demircioğlu Sinan 
Published: December 2022

Introduction Rituximab, which is widely used in the treatment of B-cell lymphoma, is a chimeric monoclonal antibody directed against the CD20 antigen. Rituximab has many side effects, mainly allergic and neurological. Rituximab may cause thrombocytopenia in the long term after administration. Rare cases of rituximab-induced acute thrombocytopenia have been reported in the literature. Case Report A 51-year-old female patient who was newly diagnosed with splenic marginal zone lymphoma received rituximab as first-line therapy. Petechiae occurred in the lower extremities on the day following rituximab administration. The blood test showed a severe drop in the platelet count from 112,000/μL to 5000/μL. Blood peripheral smear evaluation confirmed severe thrombocytopenia. Management and outcome There was no change in hemoglobin or white blood cell levels. After the diagnosis of rituximab-induced acute thrombocytopenia, thrombocyte suspension was administered due to the risk of bleeding. Close clinical and laboratory observations were made. The platelet count began to rise gradually in the following period. Before the second week of rituximab administration, the platelet count was 122,000/μL. No complications developed after premedication and slow rituximab administration, and subsequent treatments were continued in the same way. Discussion Rituximab has widespread use, especially in malignancies and autoimmune diseases. Like many monoclonal antibodies, rituximab has several side effects. Thrombocytopenia is a long-term side effect associated with rituximab, and rituximab-induced severe acute thrombocytopenia has been rarely reported. Therefore, it should be kept in mind that severe acute thrombocytopenia may develop after rituximab administration.
How Do Presenteeism and Family Functioning Affect the Association Between Chinese Nurses’ Job Stress and Intention to Stay?
Authors: Ren Zheng , Sun Yan , Li Xiangrong , He Minfu , Shi Hong , Zhao Hanfang , Li Yuyu , Pu Yajiao , Liu Hongjian , Zhang Xiumin 
Published: December 2022

Background: Despite a great deal of existing research on nurses’ job stress and intention to stay, it is still unclear how the association between Chinese nurses’ job stress and intention to stay is affected by presenteeism and family functioning. Aims: This study aimed to investigate the correlations of Chinese nurses’ job stress, presenteeism, and family functioning with their intention to stay and clarify the mediating effect of presenteeism and the moderating effect of family functioning on the association between their job stress and intention to stay. Method: A cross-sectional study of 1,008 nurses was performed in a major general hospital in Qiqihar City, Heilongjiang Province, China. The participants were invited to engage in a questionnaire survey regarding job stress, presenteeism, family functioning, and intention to stay. A moderated mediation analysis was conducted through multiple linear regression analysis and the PROCESS macro. Results: Job stress and presenteeism were negatively correlated with intention to stay, whereas family functioning was positively correlated with intention to stay. The effect of job stress on intention to stay was partially mediated by presenteeism (Effect = −0.147, 95% CI = [−0.305, −0.003]). In addition, family functioning moderated the direct effect of job stress on intention to stay (Index = −0.134, 95% CI = [−0.274, −0.001]). Conclusions: Presenteeism and family functioning were found to play mediating and moderating roles, respectively, in the relationship between job stress and intention to stay. Relevant interventions or family-friendly programs should be developed to enhance nurses’ intention to stay.
Choroidal vascularity profile in diabetic eyes using wide field optical coherence tomography
Authors: Gujar Ramkailash , Cagini Carlo , Fruttini Daniela , Corbucci Roberta , Rasheed Mohammed Abdul , Vupparaboina Kiran Kumar , Mariotti Cesare , Chhablani Jay , Lupidi Marco 
Published: December 2022

Purpose To report the wide-field choroidal vascularity up to the mid-equator area in diabetic retinopathy (DR) subjects using wide-field optical coherence tomography (WF-OCT). Design Prospective, Cross-sectional study. Participants Forty-seven eyes of 25 DR subjects. Methods WF-OCT images (55 degrees) were obtained using Spectralis HRA + OCT (Heidelberg Engineering, Germany) in extremes of gazes in all quadrants and manual montages were created to obtain wide field images up to mid equator. A previously reported semi-automated algorithm was used to calculate choroidal vascularity profile (CVI). Regression analysis was performed to identify the factors influencing CVI. Results Forty-seven eyes from 25 patients were enrolled in the study. The mean age was 68.4 ± 10.6 years. The refractive error (spherical equivalent) ranged from −2.25 to +3.75 diopters. Most common DR grade among study subjects was moderate NPDR (29.41%) and 74.5% eyes had diabetic macular edema (DME). The mean CVI in the macular area (58.29 ± 3.63) was significantly lower than in any of the other fundus areas (all p ˂ 0.01). The maximum CVI was seen in the nasal region (66.60 ± 5.61), followed by temporal (65.69 ± 3.81), superior (65.01 ± 4.87), and inferior (63.80 ± 5.42). The vertical macular area had the least coefficient of variation (CV) of CVI (0.06) while the inferior quadrant had the highest CV (0.08). Conclusion The current study describes the CVI profile on WF-OCT in DR eyes up to mid-equator. The significant increase of the CVI compared to healthy subjects and its significant regional variations introduce this novel quantitative parameter as a reliable biomarker of the diabetes-induced choroidal microangiopathy.
Severe Tortuosity of the Distal Descending Thoracic Aorta Affects the Accuracy of Distal Deployment During a Thoracic Endovascular Aortic Repair
Authors: Sato Tomohiro , Banno Hiroshi , Ikeda Shuta , Kawai Yohei , Tsuruoka Takuya , Sugimoto Masayuki , Niimi Kiyoaki , Kodama Akio , Komori Kimihiro 
Published: December 2022

Purpose: An accurate distal deployment is essential for successful thoracic endovascular aortic repair (TEVAR) of a paradiaphragmatic aortic aneurysm. This study aimed to investigate the anatomical and intraoperative factors that affect the accuracy of distal deployment during TEVAR. Methods: We conducted a retrospective review of preoperative and postoperative computed tomography scans of 426 patients undergoing TEVAR at our institution between October 2008 and May 2021, of which the stent-graft was attempted to be deployed just above the celiac axis or the superior mesenteric artery in 56 patients. Based on the anatomical factors related to the malposition (deployed >10 mm away from the target vessel) and the greater curve to the straight-line ratio (G/S ratio), the patients were categorized as severe tortuosity (n=21) and mild tortuosity (n=35) groups to compare the operative and clinical outcomes. Result: Stent-graft malpositioning occurred in 21 cases. Among all anatomical variables, only the G/S ratio was significantly larger in the malpositioned cases (p=0.049). A cutoff G/S ratio value of 1.15 was determined using the receiver operating curve analysis. In the severe tortuosity group, the distal end of the stent-graft was significantly farther (median: 10.0 [interquartile range (IQR): 2.5–19.5] mm vs 3.0 [0–8.0] mm; p=0.015) from the target vessel, and the tilt angle of the stent-graft’s distal edge was larger (median: 21.4 [IQR: 15.8–24.5] vs 9.5 [5.5–12.5] degree; p<0.01) than that in the mild tortuosity group. Both groups were comparable for the incidence of a primary type Ib endoleak (p=0.454), a secondary type Ib endoleak (p=1.0), and the rate of distal reintervention (p=0.276). Conclusion: Severe tortuosity in the distal descending thoracic aorta is associated with a malpositioned and tilted distal end of the stent-graft. Clinical Impact Thoracic endovascular aortic repair (TEVAR) for paradiaphragmatic thoracic aortic aneurysms requires accurate distal landing. In this paper, a retrospective CT analysis revealed that the greater curve to the straight-line ratio (G/S ratio) was associated to affects the malposition of the stent graft, defined as being deployed more than 10 mm away from the target vessel. Further, a comparative analysis based on the G/S ratio demonstrated that severe aortic tortuosity was associated with a more distal and tilted deployment of the stent graft.
Effects of eptinezumab on self-reported work productivity in adults with migraine and prior preventive treatment failure in the randomized, double-blind, placebo-controlled DELIVER study
Authors: Barbanti Piero , Goadsby Peter J. , Lambru Giorgio , Ettrup Anders , Christoffersen Cecilie Laurberg , Josiassen Mette Krog , Phul Ravinder , Sperling Bjørn 
Published: December 2022

AbstractBackgroundThe multinational phase 3b DELIVER trial was designed to evaluate the efficacy and safety of eptinezumab for migraine prevention in patients with prior preventive treatment failures across 17 countries. In the placebo-controlled portion, eptinezumab relative to placebo demonstrated greater reductions in migraine and headache frequency, migraine and headache severity, and acute medication use. The objective of this report is to describe the effects of eptinezumab on self-reported work productivity in the placebo-controlled portion of DELIVER.MethodsAdults 18–75 years of age with migraine and documented evidence of 2 to 4 prior preventive treatment failures in the past 10 years were randomized to receive eptinezumab 100 mg, 300 mg, or placebo intravenously (IV) every 12 weeks. The Work Productivity and Activity Impairment questionnaire specific to migraine (WPAI:M), which comprises 6 items (4 of which are completed by currently employed patients only), was administered every 4 weeks. Changes from baseline in subscores (absenteeism, presenteeism, work productivity loss, and activity impairment) were calculated based on item responses. A mixed model for repeated measures was used to analyze changes from baseline in WPAI:M subscores.ResultsA total of 890 adults (mean age, 43.8 years) were included in the full analysis set (eptinezumab 100 mg,n = 299; eptinezumab 300 mg,n = 293; placebo,n = 298). Mean WPAI:M subscores at baseline indicated a negative impact of migraine attacks on work productivity and ability to complete normal daily activities. Eptinezumab improved WPAI:M subscores more than placebo at all assessment points throughout the study. Mean changes from baseline in self-reported work productivity loss were −19.5, −24.0, and −9.7 at Week 12; and −22.6, −20.2, and −7.2 at Week 24 (allP < 0.001 vs placebo) for eptinezumab 100 mg, eptinezumab 300 mg, and placebo, respectively. Mean changes from baseline in activity impairment were −21.3, −23.8, and −11.2 at Week 12; and −24.7, −22.6, and −10.1 at Week 24 (allP < 0.0001 vs placebo). Similarly, mean improvements in absenteeism and presenteeism were greater in the eptinezumab groups than in the groups receiving placebo at all timepoints (P < 0.05).ConclusionIn adults with migraine and prior preventive treatment failure, eptinezumab 100 mg and 300 mg IV every 12 weeks improved absenteeism, presenteeism, work productivity loss, and activity impairment more than placebo.Trial registrationClinicalTrials.gov(Identifier:NCT04418765); EudraCT (Identifier: 2019–004497-25) (https://www.clinicaltrialsregister.eu/ctr-search/trial/2019-004497-25/PL).Graphical AbstractEptinezumab improves self-reported work productivity in patients with migraine and prior preventive treatment failures.
Oral mycobiota and pancreatic ductal adenocarcinoma
Authors: Wei Ailin , Zhao Huiling , Cong Xue , Wang Linyao , Chen Yiyang , Gou Juxiang , Hu Ziyi , Hu Xiuying , Tian Yali , Li Ka , Deng Yufeng , Zuo Haojiang , Fu Mei Rosemary 
Published: December 2022

AbstractEarly detection of pancreatic ductal adenocarcinoma (PDAC) is essential for survival. Preliminary research demonstrated significant associations between structural alternation of mycobiota and PDAC. In this study, we investigated the associations between oral mycobiota and PDAC. We further explored mycobiota biomarkers for PDAC detection. We enrolled 34 PDAC patients and 35 matched healthy controls from West China hospital in Southwest China. Demographic data, clinical information, and salivary samples were collected. Mycobiota characteristics were defined using Internal Transcribed Spacer (ITS) ribosomal RNA sequencing. We found that the PDAC patients had significant increase in fungal abundance (P < 0.001) and significant decrease in fungal diversity (P < 0.001) in comparison to the healthy controls. A higher abundance of Basidiomycota and Unclassifed_p_Ascomycota was associated with an increased risk of PDAC. With each increase of abundance of g__unclassified_k__Fungi and g__unclassified_p__Ascomycota in PDAC patients, the risk of pancreatic cancer increased by 1.359 odds and 1.260 odds, respectively. Aspergillus (AUC = 0.983, 95% CI 0.951–1.000) and Cladosporium (AUC = 0.969, 95% CI 0.921–1.000) achieved high classification powers to distinguish PDAC patients from the healthy controls. The rapid, inexpensive tests of ITS1 sequencing of mycobiota and PCR detection of potential fungal biomarkers make it promising for the clinical practice to use oral microbes for PDAC early detection and prevention. Results of our study provide evidence that salivary mycobiota may provide insights into cancer risk, prevention, and detection.