bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of online social evaluation on mood and cognition in young people
Authors: Grunewald Karina , Deng Jessica , Wertz Jasmin , Schweizer Susanne 
Published: December 2022

AbstractAdolescence is characterised by increased peer interactions and heightened sensitivity to evaluation by peers. Increasingly, social interactions and evaluation happen in online contexts. Yet, little is known about the impact of online social interactions and evaluation on adolescent emotional and cognitive functioning. The present study examined the impact of online social evaluative threat on young people’s mood and learning and whether this varied as a function of known offline social risk and protective factors. 255 participants completed a perceptual learning task under online social evaluative threat and a perceptually-matched control condition. Participants were aged 11–30 years, to allow for the exploration of age differences in the impact of online social evaluative threat from adolescence to early adulthood. Participants reported a greater increase in negative mood (self-reported levels of stress, anxiety, and anhedonia), following social evaluative threat compared to the control condition. Heightened social rejection sensitivity (measured using the Online and Offline Social Sensitivity Scale) and lower perceived social support (measured using the Schuster Social Support Scale) were associated with elevated negative mood across the study. Social evaluative threat adversely impacted overall accuracy on the perceptual matching task, but not learning. These findings provide preliminary evidence that online social evaluative threat impacts adolescent mood and cognitive functioning.
The biomass of bloom-forming colonial Microcystis affects its response to aeration disturbance
Authors: Wang Xiaodong , Liu Xingguo , Qin Boqiang , Tang Xiangming , Che Xuan , Ding Yanqing , Gu Zhaojun 
Published: December 2022

AbstractThe algal succession in Microcystis blooms of varying biomass under continuous aeration was studied in a greenhouse. There were four treatments (control, Low, Medium, and High) with initial chlorophyll a (Chl-a) of 32.5, 346.8, 1413.7, and 14,250.0 μg L−1, respectively. During the experiment, Cyanophyta biomass was the lowest in the Medium treatment (P < 0.05), while its Chlorophyta biomass was the highest (P < 0.05). Both Chlorophyta and Bacillariophyta biomass were the lowest in the High treatment (P < 0.05). Bacillariophyta biomass, particularly the diatom Nitzschia palea was the highest in the Low treatment (P < 0.05), and Nitzschia palea cells were attached to the Microcystis colonies. Thus, the algal shift in Microcystis blooms under aeration disturbance depends on its initial biomass, and it shift to green algae or/and diatom dominance in the control, Low, Medium treatments. Diatom cells, particularly N. palea, grew in an attached form on Microcystis colonies in treatment Low, in which the colonies provided media for the adherence. The mechanism of the algal shift with different biomass must be related to the nutrient level, low light and aerobic conditions under aeration disturbance as well as the aeration itself, which destroyed the Microcystis colonies’ advantage of floating on the water.
Metabolic enhancement of the one carbon metabolism (OCM) in bovine oocytes IVM increases the blastocyst rate: evidences for a OCM checkpoint
Authors: Golestanfar Arefeh , Niasari-Naslaji Amir , Jafarpour Farnoosh , Rouhollahi Shiva , Rezaei Naeimeh , Menezo Yves , Dattilo Maurizio , Nasr-Esfahani Mohammad Hossein 
Published: December 2022

AbstractThe one carbon metabolism (OCM) has a primary role in the process of oocyte maturation. In this study bovine oocytes were cultured for 24 h, up to MII stage, with standard medium supplemented or not with 8 metabolic enhancers of the OCM and the MII and blastocyst rate were compared. Additional analyses were performed on matured oocytes, cumulus cells, zygotes and blastocysts. The OCM supplementation increased the blastocyst rate derived from in vitro fertilization. The mitochondrial mass and DNMT3a protein expression were increased whereas DNA fragmentation decreased in matured oocytes. DNA methylation in female pronucleus of zygotes was increased. The supplementation did not directly affect the redox balance as ROS and GSH in matured oocytes and homocysteine in the spent medium were unchanged. The supplementation of the oocytes with metabolic enhancers of the OCM may increase the yield from the culture, likely due to improved DNA methylation and epigenetic programming. The lack of effects on MII rate with huge differences appearing at the blastocyst stage suggest the existence of a OCM metabolic check point that hampers oocytes progression to blastocyst post-fertilization, if they were not properly primed at the time of maturation.
Linking single nucleotide polymorphisms to signaling blueprints in abdominal aortic aneurysms
Authors: Lim Chrysania , Pratama Muhammad Yogi , Rivera Cristobal , Silvestro Michele , Tsao Philip S. , Maegdefessel Lars , Gallagher Katherine A. , Maldonado Thomas , Ramkhelawon Bhama 
Published: December 2022

AbstractAbdominal aortic aneurysms (AAA) is a multifactorial complex disease with life-threatening consequences. While Genome-wide association studies (GWAS) have revealed several single nucleotide polymorphisms (SNPs) located in the genome of individuals with AAA, the link between SNPs with the associated pathological signals, the influence of risk factors on their distribution and their combined analysis is not fully understood. We integrated 86 AAA SNPs from GWAS and clinical cohorts from the literature to determine their phenotypical vulnerabilities and association with AAA risk factors. The SNPs were annotated using snpXplorer AnnotateMe tool to identify their chromosomal position, minor allele frequency, CADD (Combined Annotation Dependent Depletion), annotation-based pathogenicity score, variant consequence, and their associated gene. Gene enrichment analysis was performed using Gene Ontology and clustered using REVIGO. The plug-in GeneMANIA in Cytoscape was applied to identify network integration with associated genes and functions. 15 SNPs affecting 20 genes with a CADD score above ten were identified. AAA SNPs were predominantly located on chromosome 3 and 9. Stop-gained rs5516 SNP obtained high frequency in AAA and associated with proinflammatory and vascular remodeling phenotypes. SNPs presence positively correlated with hypertension, dyslipidemia and smoking history. GO showed that AAA SNPs and their associated genes could regulate lipid metabolism, extracellular matrix organization, smooth muscle cell proliferation, and oxidative stress, suggesting that part of these AAA traits could stem from genetic abnormalities. We show a library of inborn SNPs and associated genes that manifest in AAA. We uncover their pathological signaling trajectories that likely fuel AAA development.
Reliable determination of pulse-shape instability in trains of ultrashort laser pulses using frequency-resolved optical gating
Authors: Jafari Rana , Khosravi Soroush D. , Trebino Rick 
Published: December 2022

AbstractWe describe a reliable approach for determining the presence of pulse-shape instability in a train of ultrashort laser pulses. While frequency-resolved optical gating (FROG) has been shown to successfully perform this task by displaying a discrepancy between the measured and retrieved traces for unstable trains, it fails if its pulse-retrieval algorithm stagnates because algorithm stagnation and pulse-shape instability can be indistinguishable. So, a non-stagnating algorithm—even in the presence of instability—is required. The recently introduced Retrieved-Amplitude N-grid Algorithmic (RANA) approach has achieved extremely reliable (100%) pulse-retrieval in FROG for trains of stable pulse shapes, even in the presence of noise, and so is a promising candidate for an algorithm that can definitively distinguish stable and unstable pulse-shape trains. But it has not yet been considered for trains of pulses with pulse-shape instability. So, here, we investigate its performance for unstable trains of pulses with random pulse shapes. We consider trains of complex pulses measured by second-harmonic-generation FROG using the RANA approach and compare its performance to the well-known generalized-projections (GP) algorithm without the RANA enhancements. We show that the standard GP algorithm frequently fails to converge for such unstable pulse trains, yielding highly variable trace discrepancies. As a result, it is an unreliable indicator of instability. Using the RANA approach, on the other hand, we find zero stagnations, even for highly unstable pulse trains, and we conclude that FROG, coupled with the RANA approach, provides a highly reliable indicator of pulse-shape instability. It also provides a typical pulse length, spectral width, and time-bandwidth product, even in cases of instability.
Loss-of-rescue of Ryr1I4895T-related pathology by the genetic inhibition of the ER stress response mediator CHOP
Authors: Germani Serena , Marchetti Alessia Celeste , Guidarelli Andrea , Cantoni Orazio , Sorrentino Vincenzo , Zito Ester 
Published: December 2022

AbstractRYR1 is the gene encoding the ryanodine receptor 1, a calcium release channel of the endo/sarcoplasmic reticulum. I4898T in RYR1 is one of the most common mutations that give rise to central core disease (CCD), with a variable phenotype ranging from mild to severe myopathy to lethal early-onset core-rod myopathy. Mice with the corresponding I4895T mutation in Ryr1 present mild myopathy when the mutation is heterozygous while I4895T homozygous is perinatal-lethal. Here we show that skeletal muscles of I4895T homozygous mice at birth present signs of stress of the endoplasmic reticulum (ER stress) and of the related unfolded protein response (UPR) with increased levels of the maladaptive mediators CHOP and ERO1. To gain information on the role of CHOP in the pathogenesis of RYR1I4895T-related myopathy, we generated compound Ryr1I4895T, Chop knock-out (-/-) mice. However, the genetic deletion of Chop, although it attenuates ER stress in the skeletal muscle of the newborns, does not rescue any phenotypic or functional features of Ryr1I4895T in mice: neither the perinatal-lethal phenotype nor the inability of Ryr1I4895T to respond to its agonist caffeine, but protects from ER stress-induced apoptosis. These findings suggest that genetic deletion of the ER stress response mediator CHOP is not sufficient to counteract the pathological Ryr1I4895T phenotype.
The role of pmrCAB genes in colistin-resistant Acinetobacter baumannii
Authors: Seleim Shaimaa Mohamed , Mostafa Marwa Salah , Ouda Nadia Hafez , Shash Rania Yahia 
Published: December 2022

AbstractThe progressively increasing antimicrobial-resistant Acinetobacter baumannii infections have enforced the use of colistin as the last option for therapy, resulting in the colistin resistance evolution. This work aimed to study the pmrCAB expression in A. baumannii isolates as well as the presence of the mcr-1 gene. Colistin MICs of 100 A. baumannii isolates were measured using the broth microdilution assay. In four colistin-susceptible and four colistin-resistant isolates, the relative expression of the pmrA, pmrB, and pmrC genes was determined using reverse transcription PCR, and then selected isolates were sequenced using the Sanger technique. Finally, the mcr-1 gene was detected using conventional PCR. The colistin resistance rate among the studied isolates was 49%. The expression levels of pmrA and pmrB were statistically significantly higher in colistin-resistant isolates than in colistin-susceptible ones, while the pmrC expression had no statistically significant change. There was a weak positive correlation between colistin MICs and the expression levels of each of the pmrA and pmrB genes. By sequencing, two colistin-resistant strains with low pmrCAB expression showed insertion mutations 3277188_3277189T in pmrB and 1185149_1185150T in pmrC. Only one isolate (1%) was positive for the presence of mcr-1. We concluded that pmrCAB increased expression and/or mutations may cause colistin resistance in A. baumannii. However, increased pmrC expression may not necessarily result in colistin resistance. In Egypt, this is the first study to reveal the existence of mcr-1 in A. baumanni. This should attract attention in clinical settings due to the ultimate tendency of spreading colistin resistance.
An iterative and interdisciplinary categorisation process towards FAIRer digital resources for sensitive life-sciences data
Authors: David Romain , Ohmann Christian , Boiten Jan-Willem , Abadía Mónica Cano , Bietrix Florence , Canham Steve , Chiusano Maria Luisa , Dastrù Walter , Laroquette Arnaud , Longo Dario , Mayrhofer Michaela Th. , Panagiotopoulou Maria , Richard Audrey S. , Goryanin Sergey , Verde Pablo Emilio 
Published: December 2022

AbstractFor life science infrastructures, sensitive data generate an additional layer of complexity. Cross-domain categorisation and discovery of digital resources related to sensitive data presents major interoperability challenges. To support this FAIRification process, a toolbox demonstrator aiming at support for discovery of digital objects related to sensitive data (e.g., regulations, guidelines, best practice, tools) has been developed. The toolbox is based upon a categorisation system developed and harmonised across a cluster of 6 life science research infrastructures. Three different versions were built, tested by subsequent pilot studies, finally leading to a system with 7 main categories (sensitive data type, resource type, research field, data type, stage in data sharing life cycle, geographical scope, specific topics). 109 resources attached with the tags in pilot study 3 were used as the initial content for the toolbox demonstrator, a software tool allowing searching of digital objects linked to sensitive data with filtering based upon the categorisation system. Important next steps are a broad evaluation of the usability and user-friendliness of the toolbox, extension to more resources, broader adoption by different life-science communities, and a long-term vision for maintenance and sustainability.
Algal sensitivity to nickel toxicity in response to phosphorus starvation
Authors: El-Agawany Nagwa I. , Kaamoush Mona I. A. 
Published: December 2022

AbstractIn all living cells, phosphorus plays an important function in the transport of metabolic energy and as a structural component of nucleotide and phospholipid molecules. Ni2+ is found in industrial water effluent and has the potential to harm aquatic ecosystems. The study was designed to assess the effect of phosphorous-limiting conditions in combination with the absence or presence of different concentrations of nickel on growth, pigment content, photosynthesis, and respiration activities of the studied alga Dunaliella tertiolecta. The EC50 for dissolved nickel was around 15 mg/L. Results obtained showed that, growth and chlorophyll content under phosphorus limiting conditions increased under low concentrations of dissolved nickel. The amount of O2-evolution with phosphorus limiting conditions was lower than those of untreated normal cultures. Lower dissolved nickel concentrations resulted in higher photosynthesis rates in the treated phosphorus-starved cultures than higher concentrations. The degree of response to metal toxicity in phosphorus-starved medium is depending mainly on the concentration of the element and the length of the culturing period and it was less than those in normal control culture containing phosphorus.
Use of Symptom Checklist 90 for exploring psychological factors among the parents of children hospitalized for burn injuries in Shanghai: a cross-sectional study
Authors: Chen Shijie , Jiang Qiuping , Zhang Yin , Zeng Changjuan 
Published: December 2022

AbstractTo better improve the conditions for the recovery of children with burn injuries, timely understanding of the psychological status of parents is important. A cross-sectional survey on it using convenience sampling was conducted at two hospitals. Besides basic information, the Symptom Checklist 90, Eysenck Personality Questionnaire, Social Support Rate Scale, and Simplified Coping Style Questionnaire were used, and the key factors were identified via multivariate linear regression analysis and path analysis. A total of 196 guardians were recruited, 180 valid and completed questionnaires were obtained, including 58 men (32.2%) and 122 women (67.8%), and their average age was 30.3 years (standard deviation = 7.6). Of these, 151 participants (83.9%) were parents. Multivariate analysis revealed that children’s age, parent gender, P score, negative coping style, and religion were the main factors that affected parents’ psychology. Moreover, path analysis showed that P score, children’s age, and negative coping style had the greatest impact on the total average score. These results suggest that during hospitalization, the following three factors should be focused on: older children, higher parental psychoticism, and increased negative coping style.
Machine-learning-based risk stratification for probability of dying in patients with basal ganglia hemorrhage
Authors: Guo Lili , Lei Nuoyangfan , Gao Mou , Qiu Wenqiao , He Yunsen , Zhao Qijun , Xu Ruxiang 
Published: December 2022

AbstractTo confirm whether machine learning algorithms (MLA) can achieve an effective risk stratification of dying within 7 days after basal ganglia hemorrhage (BGH). We collected patients with BGH admitted to Sichuan Provincial People’s Hospital between August 2005 and August 2021. We developed standard ML-supervised models and fusion models to assess the prognostic risk of patients with BGH and compared them with the classical logistic regression model. We also use the SHAP algorithm to provide clinical interpretability. 1383 patients with BGH were included and divided into the conservative treatment group (CTG) and surgical treatment group (STG). In CTG, the Stack model has the highest sensitivity (78.5%). In STG, Weight-Stack model achieves 58.6% sensitivity and 85.1% specificity, and XGBoost achieves 61.4% sensitivity and 82.4% specificity. The SHAP algorithm shows that the predicted preferred characteristics of the CTG are consciousness, hemorrhage volume, prehospital time, break into ventricles, brain herniation, intraoperative blood loss, and hsCRP were also added to the STG. XGBoost, Stack, and Weight-Stack models combined with easily available clinical data enable risk stratification of BGH patients with high performance. These ML classifiers could assist clinicians and families to identify risk states timely when emergency admission and offer medical care and nursing information.
Observation of prior light emission before arcing development in a low-temperature plasma with multiple snapshot analysis
Authors: Kim Si-jun , Lee Young-seok , Cho Chul-hee , Choi Min-su , Seong In-ho , Lee Jang-jae , Kim Dae-woong , You Shin-jae 
Published: December 2022

AbstractArcing is a ubiquitous phenomenon and a crucial issue in high-voltage applied systems, especially low-temperature plasma (LTP) engineering. Although arcing in LTPs has attracted interest due to the severe damage it can cause, its underlying mechanism has yet to be fully understood. To elucidate the arcing mechanism, this study investigated various signals conventionally used to analyze arcing such as light emission, arcing current and voltage, and background plasma potential. As a result, we found that light emission occurs as early as 0.56 μs before arcing current initiation, which is a significant indicator of the explosive development of arcing as well as other signals. We introduce an arcing inducing probe (AIP) designed to localize arcing on the tip edge along with multiple snapshot analysis since arcing occurs randomly in space and time. Analysis reveals that the prior light emission consists of sheath and tip glows from the whole AIP sheath and the AIP tip edge, respectively. Formation mechanisms of these emissions based on multiple snapshot image analysis are discussed. This light emission before arcing current initiation provides a significant clue to understanding the arcing formation mechanism and represents a new indicator for forecasting arcing in LTPs.
Several machine learning techniques comparison for the prediction of the uniaxial compressive strength of carbonate rocks
Authors: Hassan Mohamed Yusuf , Arman Hasan 
Published: December 2022

AbstractIn engineering practices, it is critical and necessary to either measure or estimate the uniaxial compressive strength (UCS) of the rock. Measuring the UCS of rocks requires comprehensive studies in the field and in the laboratory for the rock block sampling, coring, and testing. These studies are time-consuming, expensive and go through difficult processes. Alternatively, the UCS can either be estimated by empirical relationships or predictive models with various measured mechanical and physical parameters of the rocks. Previous studies used different methods to predict UCS, including least squares regression techniques (MLR), adaptive neuro-fuzzy inference system (ANFIS), Sequential artificial neuron networks (SANN), etc. This study is intended to estimate the UCS of the carbonate rock by using a simple, measured Schmidt Hammer (SHVC) test on core sample and a unit weight (γn) of carbonate rock. Principal components regression (PCR), MLR, SANN, and ANFIS are employed to predict the UCS. We are not aware of any study compared the performances of these methods for the prediction of the UCS values. Based on the root mean square error, mean absolute error and R2, the Sequential artificial neural network has a slight advantage against the other three models.
Behavioral roles of biogenic amines in bumble bee males
Authors: Watanabe Tomohiro , Sasaki Ken 
Published: December 2022

AbstractTo compare the behavioral roles of biogenic amines in the males of primitive and advanced eusocial bees, we determined the levels of dopamine- and octopamine-related substances in the brain, and the behavioral effects of these monoamines by drug injection in the primitive eusocial bumble bee, Bombus ignitus. The levels of dopamine and its precursors in the brain peaked at the late pupal stage, but the dopamine peak extended to adult emergence. The tyramine and octopamine levels increased from the mid-pupal to adult stages. The locomotor and flight activities, and light preference increased with age. Injection of octopamine and its receptor antagonist had significant effects on the locomotor and flight activities, whereas dopamine injection did not, indicating that these activities can be regulated by the octopaminergic system. We also determined the dynamics of dopamine-related substances in honey bee (Apis mellifera) drones. The changes in the dopamine level in the brains of honey bee drones exhibited two peaks from the pupal to adult stages, whereas the bumble bee males had only one peak. These are consistent with the behavioral functions of dopamine in honey bee drones and ineffectiveness of dopamine injection at the adult stage in bumble bee males.