bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of surface CO2 sources and wind transport on atmospheric CO2 variability over the Bay of Bengal during Southwest Monsoon - using mixing ratio, carbon isotope ratio and model simulated wind field
Authors: Guha Tania , Ghosh Subhomoy , Raghava Yakkala Yagnesh , Bhat Ganapati Shankar , Ghosh Prosenjit , Tiwari Yogesh , Kumar Kunchala Ravi 
Published: August 2022

Abstract
The present study examines the role of surface-CO2 sources and wind transport on atmospheric CO2 observations over the Bay of Bengal. The ship-based observations of mixing ratio and stable carbon isotope ratio (δ13C) of air CO2 was carried out at coastal and open ocean location during 17 July 2009 until 18 August 2009, covering the peak months of the Southwest Monsoon season. The average mixing ratio was higher for the coastal sites (407 ± 17 µmol mol− 1) than the open ocean sites (394 ± 9 µmol mol− 1), whereas the average δ13C value was lower (more depleted in 13C) (-8.75 ± 0.53‰) for the coastal sites than the open ocean sites (-8.48 ± 0.04‰). The observations were further used to identify the isotopic ratio of the source CO2 using the Keeling and the Miller-Tans plot. The presence of two different sources were confirmed from the observations. The coastal observations were affected by continental sources of CO2 (-19.95 ± 1.7‰), whereas for open ocean sites, oceanic sources (-10.31 ± 0.15‰) played a major role. Although there was a transport of continental air to open ocean, as observed from the WRF model simulated wind, the land-ocean contrast was maintained. It was evident from the Carbon Tracker simulation during the study period. Despite continental air transport, the open ocean observation was influenced by oceanic sources of CO2. To explore the reason, the predominant wind pattern for the sampling sites were determined from the WRF model simulated winds. A k-means clustering technique was used on the WRF model simulated hourly wind to understand the different atmospheric transport patterns during different days of sampling in July and August. Most of the sampling sites were resided within the clusters whose mean wind direction indicates south-westerly/southerly wind. Thus, irrespective of continental transport, the sampling sites were influenced by oceanic wind during July and August. The influence of oceanic wind allowed the identification of oceanic source CO2 over the open ocean sites despite the transport from the continent.
CCDC85A is regulated by miR-224-3p and augments cancer cell resistance to endoplasmic reticulum stress
Authors: Tanaka Masamitsu , Takagane Kurara , Itoh Go , Kuriyama Sei , Umakoshi Michinobu , Goto Akiteru , Iijima Katsunori , Takahashi So , Yanagihara Kazuyoshi , Yashiro Masakazu 
Published: August 2022

Abstract
MicroRNAs (miRNAs) play pivotal roles in the tumor microenvironment. Here, we analyzed miRNAs in tumor stromal fibroblasts. Expression of miR-224-3p in cancer-associated fibroblasts (CAF) from scirrhous gastric cancer patients was lower than in normal fibroblasts (NF). Introduction of a miR-224-3p mimic attenuated migration and invasion of CAF. By contrast, a miR-224-3p inhibitor augmented migration of NF. Coiled-coil domain containing 85A (CCDC85A), whose function in tumors is not understood, was the target gene of miR-224-3p. Immunohistological analysis revealed that CCDC85A is expressed to varying degrees by cancer cells and CAFs in gastric and pancreatic carcinomas. Down-regulation of CCDC85A in cancer cells revealed that these cells are vulnerable to the ER-stress induced by thapsigargin or tunicamycin. Expression of CCDC85A in cancer cells fell upon exposure to NF-derived exosomes containing miR-224-3p, resulting in ER stress-mediated apoptosis and increased tumor shrinkage after cisplatin treatment. All these events were ameliorated after addback of CCDC85A. Mechanistically, CCDC85A associated with the molecular chaperone GRP78 and GRP94, thereby inhibiting association of these negative regulators of unfolded protein response (UPR) with PERK, leading to sustained activation of PERK upon ER-stress. Consistent with this, events downstream of PERK activation including phosphorylation of eIF2α and increased expression of ATF4 were suppressed in CCDC85A knockout cells. These data suggest a novel function of CCDC85A regulated by miR-224-3p in tumors: protection from ER stress and cisplatin resistance.
Development and initial psychometric testing of a scale for ICU nurses' knowledge, belief, and behavior in critically ill patients with post-ICU syndrome
Authors: cheng zhiqiang , Zhang Baozhen , xia jiaoyun , wang xiaoxing 
Published: August 2022

Abstract
Background: Post-ICU syndrome has not been paid enough attention in Chinese medical institutions. Due to the large population base in my country, the number of people with post-ICU syndrome is also large, which has a great impact on the quality of life of patients in the later period. Objectives To develop and verify ICU nurses' knowledge, attitude and practice scale on post-ICU syndrome in critically ill patients, to provide a basis for the investigation of post-ICU syndrome status. Methods Using the principle of Knowledge, Attitude and Practice (KAP), a scale item database was created through literature review and semi-structured interviews. The scale was modified by Delphi expert correspondence method and pre-investigation. 451 ICU nurses were selected for formal investigation, and the reliability of the scale was evaluated degree test. Results A scale of knowledge, attitude, and practice of ICU nurses on post-ICU syndrome in critically ill patients was formed, In which the number of items in the three dimensions of knowledge, attitude, and practice were 11, 7, and 13, respectively. The Pearson correlation coefficient between dimensions and the total score of the scale ranged from 0.526 to 0.860, and the correlation coefficients between items and dimensions ranged from 0.517 to 0.921 (P＜0.001). Item content validity (I-CVI) ranged from 0.867 to 1, and scale content validity (S-CVI) is 0.989. Four common factors were extracted by exploratory factor analysis, and the cumulative variance explanation rate was 76.214%. Confirmatory factor analysis: Chi-square ratio of degrees of freedom (χ2/DF) =2.789, RMSEA=0.077, RMR=0.048, GFI=0.947, CFI=0.922, NFI=0.943, IFI=0.922, TLI=0.915. The Cronbach's alpha coefficients of the scale and dimension were 0.948, 0.976, 0.966, 0.910, split-half reliability = 0.951, and re-test reliability=0.927. Conclusion The reliability and validity of the scale meets the requirements of scale preparation, and can reflect the knowledge, belief, and behavior of ICU nurses in post-ICU syndrome.Relevance to clinical practice In this study, we developed a post-ICU syndrome knowledge, belief, and behavior survey to understand the current status of post-ICU syndrome knowledge, belief, and behavior among ICU nurses in my country.
Dynamic changes of DNA modifications: 5-hydroxymethyluracil and N6-methyladenine in the Drosophila melanogaster life cycle and in response to environmental factors
Authors: Starczak Marta , Gawronski Maciej , Wasilow Aleksandra , Mijewski Pawel , Olinski Ryszard , Gackowski Daniel 
Published: August 2022

Abstract
In this study, the level of DNA modifications was investigated in three development stages of Drosophila melanogaster (larvae, pupae, imago) and in an in vitro model (Schneider 2 cells). The analysis was carried out using the two-dimensional ultra-performance liquid chromatography with tandem mass spectrometry. Our method made it possible for the first time to analyze a broad spectrum of DNA modifications in the three stages of the Drosophila. Each stage was characterized by a specific modification pattern and the level of these compounds fluctuated throughout the D. melanogaster life cycle. The level of the DNA modification was also compared between insects bred at 25°C (optimal temperature) and at 18°C, the conclusion being that both groups differed significantly. The profound changes of N6-methyladenine and 5-hydroxymethyluracil levels during the Drosophila life cycle and as a result of bred temperature changes indicate that these DNA modifications can play important regulatory roles in response to environmental changes and/or biological conditions. Moreover, the supplementation of Schneider 2 cells with 1mM L-ascorbic acid caused a time-dependent increase in the level of 5-(hydroxymethyl)-2’-deoxyuridine. These data suggest that a certain pool of this compound may arise from the enzymatic activity of dTET protein.
Mechanism of competitive binding of miR-106b-5p by circRNA-ITCH to target and regulate SETD2 to inhibit prostate cancer progression
Authors: Chen Guo , Qu Lijun , Mo Rujun , Ling Muan , Yin Wenjun , Chen Zheng , Pan Bin 
Published: August 2022

Abstract
Bacnkground Approximately 15% of patients with prostate cancer (PCa) are diagnosed with high-risk disease and will face sequelae of diagnosis and treatment. Recently, aberrant expression of circular RNA-ITCH (circ-ITCH) may be associated with the risk of lymph node metastasis and poorer prognosis in tumor patients. The mechanism underlying the role of circ-ITCH in the progression and development of PCa is still under investigation.Objective To elucidate the functional role of the circ-ITCH gene in PCa, assess its possible predictive/prognostic molecular biomarkers of PCa, and possible molecular mechanisms of action.Methods In this study, we collected six sets of paired PCa and normal tissue specimens to explore the relationship between circ-ITCH expression and PCa development; Cell growth was assessed by the Cell Counting Kit-8 assay and cell clone formation assay; Transwell assays were performed to evaluate cell migration and invasion; Apoptotic cells were detected by Annexin-V-isothiocyanate fluorescein and propidium iodide staining; Target gene prediction and screening, luciferase reporter gene assays were utilized to confirm the relationship between circ-ITCH and microRNA.Results In the present study, we found that circ-ITCH, is down-regulated in PCa tissues and cell lines. Moreover, enforced-expression of circ-ITCH inhibited cell proliferation, migration, invasion and metastasis in vitro . Mechanistically, we demonstrate that circ-ITCH upregulates the expression of the target gene SETD2 by 'sponging' miR-106b-5p, which suppressed the aggressive biological behaviors of PCa.Conclusions Circ-ITCH inhibits PCa cell proliferation and metastasis by negatively regulating the miR-106b-5p/SETD2 axis.
Intrinsic dense twinning via release of native strain
Authors: Song Xianqi , Liu Chang , Li Quan , Ma Yanming , Chen Changfeng 
Published: August 2022

Abstract

Icosahedral boron-rich solids exhibit outstanding mechanical properties, but the crystal structure of this prominent class of materials has long remained enigmatic. Here, we report on a surprising discovery of an unprecedented twinning induced structural stabilization that creates highly twinned crystal structures that are stabler than the prevailing single crystal structure comprising complex multi-atom structural units. This phenomenon is showcased via a symmetry guided optimization process that produces a series of increasingly twinned B4C structures with progressively lower energy below that of the single crystal, and this behavior also occurs in multiple other boron-rich solids. These findings unveil a distinct paradigm of defect (twinning) induced structural stabilizing mechanism that reduces energy via release of native strains built in the complex structural units of the single crystal, creating an exceptional category of materials that comprise multiple domains of intrinsic dense twinning in the crystal structures.
Synthesis, Characterization and Antimicrobial Activity of Some New Benzimidazole Derivatives Bearing the Salicyl Moiety
Authors: Gürbüz Demet , Tavman Aydin , Çınarlı Adem , Tan A. Seher Birteksöz 
Published: August 2022

Abstract
Sixteen new benzimidazole compounds bearing the salicyl moiety have been synthesized by cyclization of 4,5-dichloro/dimethyl-1,2-phenylenediamine with various salicylaldehydes. All the compounds were characterizated by spectral methods (FT-IR, 1H NMR, ESI-MS and fluorescence spectroscopy) and elemental analysis. It is seen that the dichloro derivatives and 5-substitution of the phenolic ring shift the fluorescence radiation (emission) to the higher wavelength (red shift). Antimicrobial activities of the compounds were screened against six bacteria and a fungus. The dimethyl derivatives (9–16) are generally more active towards the microorganisms and show higher antifungal activity than the dichloro derivatives. The compound 13, which is effective against all microorganisms, shows the broadest activity.
Lisinopril Associated with Non-Pemphigus Bullae Lesions
Authors: Callahan Meghan , Sehmi Kirandeep , Hostoffer Robert W. 
Published: August 2022

Abstract
Background: Angiotensin-converting enzyme inhibitors are first-line drugs for patients suffering from hypertension. These drugs have common adverse effects including bronchospasm, cough, and angioedema. Drug-induced bullous eruptions have been reported previously with numerous drugs including lisinopril, an angiotensin-converting enzyme inhibitor. Three patients of note have had a form of biopsy-proven pemphigus induced by the introduction of lisinopril or an increase in dosage. We report a case of lisinopril-induced bullae that is not pemphigus foliaceus or bullous pemphigoid verified by biopsy.Case Presentation: A 65-year-old male presented with a blistering rash over his left flank, belly button and with a cluster on his ankle. His medical history is significant for hypertension, which was controlled by 20mg of lisinopril, which had been increased to 40mg in order to provide better control. A skin biopsy was done on one of the rashes with a histological report of a bullous reaction with a low possibility of bullous pemphigoid and probably caused by arthropod bite or drug hypersensitivity. Within a month of dosage change the patient started developing bullae across his body, with no relief from oral steroid administration. The patient was taken off lisinopril and within days the bullae started to heal and there were no new eruptions. Conclusion: Pemphigus is an autoimmune condition that causes blistering of the skin and mucous membranes and can be triggered by the environment, genetics or vaccination. Three patients have been reported to have a biopsy-proven pemphigus reaction caused by the introduction of lisinopril or an increase in dosage. These blisters were classified as pemphigus after biopsy and pathological analysis. These cases were resolved with removal of the medication from their regime along with topical creams to aid in healing. There is no known study published of a lisinopril-induced non-pemphigus bullae which our patient presented with after the doubling of his lisinopril dose and a pathology report stating a low likelihood of bullous pemphigoid.
Vitamin D level is associated with increased clinical pregnancy rate and live birth rate in Chinese women undergoing IVF/ICSI-ET: a retrospective cohort study
Authors: Yu Zhengchen , Sun Yiqun , Wang Peng , Hu Yinkai , Zhou Yuxi , Xie Jinrui , Lu Zhenzhen , Xu Luxuan , Zhao Junzhao , Chi Haihong 
Published: August 2022

Abstract
Research questionTo explore the relationship between serum 25(OH)D level and Clinical Pregnancy Rate (CPR) as well as Live Birth Rate (LBR) in Chinese women receiving IVF/ICSI-ET.DesignThe data of 612 patients who underwent IVF/ICSI-ET in the Reproduction Center of the Second Affiliated Hospital of Wenzhou Medical University were retrospectively analyzed. ResultsNone of the baseline characteristics of participants were significantly different between the two cohorts. The trend of 25(OH)D concentration was positively correlated with CPR and LBR. Moreover, our results indicated that the younger (Age: p<0.001 both in CPR and LBR) women with primary infertility (Infertility type: p=0.004 in LBR) were more likely to get a better pregnancy outcome under the same 25(OH)D concentration stages. As shown on heatmap plots, CPR and LBR were significantly increased for 25(OH)D concentrations above 30.00ng/ml and women younger than 30 years old. The adjusted binary logistic regression and RCS showed that there exists a nonlinear positive correlation between 25(OH)D concentration and pregnancy outcome (CPR and LBR) (nonlinear < 0.001, respectively). The women with a sufficient 25(OH)D concentration (30ng/ml) had 1.07 (clinical pregnancy) and 1.05 (live birth) times higher successful birth outcomes compared to women with an insufficient 25(OH)D concentration (25ng/ml). (OR25ng/ml,ref=30ng/ml [95%CI] = 0.935[0.932-0.938] and 0.947[0.945-0.950],P<0.001, respectively)ConclusionIn Chinese women receiving IVF/ICSI-ET, the serum level of 25(OH)D demonstrated a non-linear positive correlation with pregnancy outcome（CPR&LBR, with stronger correlations above 25 ng/ml and worse yields below 30 ng/ml. However, it could not yet be considered different in distinct ages.
Tissue sheet engineered using human umbilical cord-derived mesenchymal stem cells improves diabetic wound healing
Authors: Zhang Jingbo , Qu Xiang , Li Junjun , Harada Akima , Hua Ying , Yoshida Noriko , Ishida Masako , Sawa Yoshiki , Liu Li , Miyagawa Shigeru 
Published: August 2022

Abstract
Background: Diabetic foot ulceration is a common chronic diabetic complication. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have been widely in regenerative medicine used owing to their multipotency and wide range of sources. In this study, we developed an hUC-MSC tissue sheet by combining hUC-MSCs and a poly (lactic-co-glycolic acid) (PLGA)-based scaffold. Methods: hUC-MSC tissue sheets were formed and optimized by culturing hUC-MSCs on PLGA-based scaffolds. Cell apoptosis, collagen composition, and tissue sheet characterization were evaluated by immunostaining in vitro . Diabetic mice were subjected to a full-thickness skin biopsy experiment to generate diabetic wounds and treated with either the hUC-MSC tissue sheet, hUC-MSC injection, fiber only, or left untreated (control). Fourteen days after transplantation, wound area tissue was collected for histological and immunohistochemical analyses. Results: The in vitro data showed that hUC-MSC tissue sheets formed thick and solid tissue sheets with abundant extracellular matrix (ECM). Additionally, hUC-MSC tissue sheet transplantation promoted diabetic wound healing in vivo , with improved re-epithelialization and collagen deposition. Remarkable blood vessel formation and maturation were also observed in the hUC-MSC tissue sheet group compared to the other groups. Furthermore, hUC-MSC transplantation alleviated inflammation in vivo . Conclusion: Our findings suggest that hUC-MSCs cultured on PLGA scaffolds improve diabetic wound healing, collagen deposition, and angiogenesis . These data provide a novel and effective method for cell transplantation, and a new strategy for the treatment of diabetic skin wounds.
T-2 toxin inhibits osteoblastic differentiation and mineralization involving mutual regulation between Wnt signaling pathway and autophagy
Authors: Zhang Jian , Wu Xia , Song Miao , Zhang Xuliang , Huo Siming , Du Jiayu , Li Bo , Cao Zheng , Li Yanfei F. 
Published: August 2022

Abstract
Mycotoxins are most frequent contaminants in environment and agricultural production globally. The T-2 toxin of Fusarium species is the most toxic type of A trichothecene mycotoxins. T-2 toxin can accumulate in bone and cause bone development disorders. Osteoblast is the functional cell responsible for bone formation. Whereas, the mechanism of T-2 toxin toxicity on osteoblast remains unknown. In present study, MC3T3-E1 cells were used to investigate the effect of T-2 toxin on differentiation and mineralization and potential underlying mechanism. The results showed that T-2 toxin inhibited both osteoblastic differentiation and mineralization. T-2 toxin repressed Wnt signaling pathway both during osteoblastic differentiation and mineralization. T-2 toxin similarly promoted autophagy during osteoblastic differentiation, while inhibited it during osteoblastic mineralization. In addition, the activation of Wnt signaling pathway mitigated T-2 toxin-induced osteoblast impairment, while the inhibition of autophagy exacerbated it. Our results also indicated that there was a positive feedback loop between the Wnt signaling pathway and autophagy. This study can provide a better understanding for relieving T-2-related bone diseases.
Anxiety, Attitudes, and Education about Fertility Among Medical Students in the United States
Authors: Smith D. Grace , Ross Abigail , HogenEsch Elena , Okine Rachel , Bonus Marissa L. , Feinberg Eve C. , Bernardi Lia A. 
Published: August 2022

Abstract
Background: As delayed family building is common among physicians, the goal of this study was to evaluate childbearing plans, anxiety related to future fertility, and interest in fertility education among medical students.Methods: An electronic REDCap survey was distributed using social media and group messaging applications to medical students enrolled in medical schools across the United States. Answers were collected, and analysis of the descriptive statistics was performed.Results: The survey was completed by 175 participants, 72% of which were female (assigned at birth). The mean (± SD) age of participants was 24.9 ± 1.9 years. Of all participants, 78.3% desire to have children and 65.1% of these individuals plan to delay childbearing. On average, the planned age of first pregnancy is 31.0 ± 2.3 years. “Lack of time” was the greatest influence on decision regarding timing of childbearing. Of all respondents, 58.9% reported at least some anxiety related to future fertility. When females and males were compared, significantly more females (73.8%) versus males (20.4%) reported worrying about future fertility (p<0.001). Participants reported that greater knowledge about infertility and potential treatments would help ease fertility related anxiety, and 66.9% of respondents showed interest in learning about how things such as age and lifestyle can impact fertility, preferably through medical curricula, videos, and podcasts. Conclusion: A majority of the medical students in this cohort intend to have children and most plan to delay childbearing. A large percentage of female medical students reported anxiety related to future fertility, but many students showed interest in receiving fertility education. This study highlights an opportunity for medical school educators to incorporate targeted fertility education into their curriculum with the intention of decreasing anxiety and improving future reproductive success.