bluuebunny/crossref_metadata_2025_split huggingface dataset:
Applying Layer-by-Layer Photo-Crosslinking in Green Bioprinting: Shape Fidelity and Cell Viability of Printed Hydrogel Constructs Containing Algae Cells
Authors: Thakare Ketan , Jerpseth Laura , Pei Zhijian , Qin Hongmin 
Published: March 2022

Abstract
Three-dimensional printing of hydrogel constructs containing algae cells, also known as green bioprinting, has many potential applications. Its feasibility has been demonstrated for removing metal contaminants in water and tissue engineering. However, in reported studies on green bioprinting so far, shape fidelity was either not investigated or poor. This paper reports a study to apply layer-by-layer photo-crosslinking in extrusion-based 3D printing of constructs using gelatin methacryloyl (GelMA) bioink containing Chlamydomonas reinhardtii algae cells. Shape fidelity and cell viability are compared between constructs printed by applying layer-by-layer photo-crosslinking and those printed by applying once-post-printing photo-crosslinking. Comparisons of shape fidelity are also made with alginate-methylcellulose constructs printed by applying ionic-crosslinking. Results show that GelMA constructs printed by applying layer-by-layer photo-crosslinking have the highest shape fidelity among all printed constructs, and about three-quarters of the algae cells in all printed GelMA constructs are alive four days post-printing. There are no significant differences in cell viability between the GelMA constructs printed by applying layer-by-layer photo-crosslinking and the GelMA constructs printed by applying once-post-printing photo-crosslinking.
Prediction of Remaining Useful Life Using Fused Deep Learning Models: A Case Study of Turbofan Engines
Authors: Zheng Yu , Bao Xiangyu , Zhao Fei , Chen Chong , Liu Ying , Sun Bo , Wang Haotong 
Published: March 2022

Abstract
The study of intelligent operation and maintenance methods for turbofan engines is of great importance for improving the reliability of turbofan engines. Given the harsh operating conditions and complex structure of the turbofan engine, it is extremely difficult to establish an accurate physical model for remaining useful life (RUL) prediction. The traditional operation and maintenance method based on the physical model has several limitations in the application of turbofan engines, while the data-driven method offers a new solution. Compared with traditional machine learning models, deep learning models possess more powerful nonlinear expression capabilities and feature extraction capabilities. Therefore, this study focuses on studying the RUL prediction algorithm for turbofan engines based on the fused deep learning models. In this article, a multimodal deep learning approach based on a 1DCNN (1D convolutional neural network) + attention enhanced Bi-LSTM (bidirectional long short-term memory) network is proposed to predict the RUL by mining the temporal information of data. Furthermore, a DDResNet (dilated deep residual network) is also introduced to the 1DCNN submodel to leverage its hidden pattern mining capability due to its advance in preventing performance degradation across layers. Subsequently, the output of these two submodels is weighted fused to obtain the final RUL prediction. The merits of the proposed method are demonstrated by comparing it with existing methods for RUL prediction using the C-MAPSS (commercial modular aero-propulsion system simulation) dataset.
Temporomandibular Joint Disorder and Biomechanical Simulation of the Replacement: A Literature Review on Various Surgical and Nonsurgical Methods, and Development of the Finite Element Method Approach in the Treatment
Authors: Banerjee Anik , Roy Chowdhury Amit , Majumder Santanu 
Published: March 2022

Abstract
While this study is on the clinical development of temporomandibular joint disorders and understanding various biomechanical approaches through finite element analysis as a panacea, the primary aim is to perform a literature review. Usage of the biomedical prosthesis, over the years, including the initial development and later modernization with image processing and computer simulations, are briefly discussed from 1800 to 2021. Manual data handling was used to select 503 journal papers, of which 116 were further studied in this review. This review explains the complete biomechanical structure of the temporomandibular joint (TMJ), as it disclosed possible reasons for the disorders and shortcomings in the medical treatments. In the Boundary Condition and Discussion sections, the paper discussed the boundary conditions and the results of various studies from 2000 to 2021. It also discussed 36 papers with temporomandibular joint replacement (TMJR) devices, providing important information on the stress–strain distributions in the implant and the mandible-fossa component. Both custom and stock devices are discussed with a finite element method (FEM) approach, with a proper evaluation of multiple software for individual studies. Finally, TMJR devices are considered helpful for treating TMJ disorders. The FEM approach should be considered a precise and valuable biomechanical simulation tool for modifying and improving next-generation prosthetic devices.
Synthesis and Antimicrobial Activity of Novel Hydrazone and 1,2,4-Triazole-3-thione Derivatives
Authors: Yankin A. N. , Nosova N. V. , Novikova V. V. , Gein V. L. 
Published: March 2022

Abstract
Novel hydrazone and 1,2,4-triazole-3-thione derivatives were obtained via the reaction of N1,N3,2-triaryl-6-hydroxy-6-methyl-4-oxocyclohexane-1,3-dicarboxamides with acid hydrazides and thiosemicarbazide, respectively. Structure of the products was proved using IR and 1H NMR spectroscopy methods. Some of the synthesized compounds were tested for antimicrobial activity
Synthesis and Antitumor Activity of 5-Phenyl-1,3-thiazole-4-sulfonamide Derivatives
Authors: Severin A. O. , Pilyo S. G. , Potikha L. M. , Brovarets V. S. 
Published: March 2022

Abstract
A method for the synthesis of 5-phenyl-1,3-thiazole-4-sulfonyl chloride was developed based on the cyclization of ethyl 2-{[1-(benzylsulfanyl)-2-oxo-2-phenylethyl]amino}-2-oxoacetate obtained from available reagents under the action of the Lawesson’s reagent and oxidative chlorination of the intermediate benzyl 5-phenyl-1,3thiazol-4-ylsulfide. The resulting sulfonyl chloride was converted into a series of 5-phenyl-1,3-thiazole-4-sulfonamide derivatives for which in vitro antitumor activity screening studies were performed on 60 cancer cell lines.
PERSIAN Traffic Safety and Health Cohort: a study protocol on postcrash mental and physical health consequences
Authors: Sadeghi-Bazargani Homayoun , Shahedifar Nasrin , Somi Mohammad Hossein , Poustchi Hossein , Bazargan-Hejazi Shahrzad , Asghari Jafarabadi Mohammad , Sadeghi Vahideh , Golestani Mina , Pourasghar Faramarz , Mohebbi Iraj , Ahmadi Sajjad , Shafiee-Kandjani Ali Reza , Ala Alireza , Abdi Salman , Rezaei Mahdi , Farahbakhsh Mostafa 
Published: March 2022

BackgroundCohort studies play essential roles in assessing causality, appropriate interventions. The study, Post-crash Prospective Epidemiological Research Studies in IrAN Traffic Safety and Health Cohort, aims to investigate the common health consequences of road traffic injuries (RTIs) postcrash through multiple follow-ups.MethodsThis protocol study was designed to analyse human, vehicle and environmental factors as exposures relating to postcrash outcomes (injury, disability, death, property damage, quality of life, etc). Population sources include registered injured people and followed up healthy people in precrash cohort experienced RTIs. It includes four first-year follow-ups, 1 month (phone-based), 3 months (in-person, video/phone call), 6 and 12 months (phone-based) after crash. Then, 24-month and 36-month follow-ups will be conducted triennially. Various questionnaires such as Post-traumatic Stress Disorder Questionnaire, Patient Health Questionnaire, WHO Disability Assessment Schedules, Cost-related Information, etc are completed. Counselling with a psychiatrist and a medical visit by a practitioner are provided accompanied by extra tools (simulator-based driving assessment, and psychophysiological tests). Through preliminary recruitment plan, 5807, 2905, 2247 and 1051 subjects have been enrolled, respectively at the baseline, first, second and third follow-ups by now. At baseline, cars and motorcycles accounted for over 30% and 25% of RTIs. At first follow-up, 27% of participants were pedestrians engaged mostly in car crashes. Around a fourth of injuries were single injuries. Car occupants were injured in 40% of collisions.DiscussionThe study provides an opportunity to investigate physical-psychosocial outcomes of RTIs, predictors and patterns at follow-up phases postinjury through longitudinal assessments, to provide advocates for evidence-based safety national policy-making.
Prior arm-crank exercise has no effect on postprandial lipaemia in nondisabled adults
Authors: Farrow Matthew T. , Maher Jennifer , Oaten Jack R. , Kreutzfeldt Saskia , Thompson Dylan , Bilzon James L.J. 
Published: March 2022

A single bout of cycling or running performed in the evening can reduce postprandial lipaemia (PPL) the following morning, although this is currently unknown for upper-body exercise. The aim of this study was to determine if a bout of arm-crank exercise (high-intensity interval [HIIE] or moderate-intensity continuous [MICE]), can attenuate PPL in noninjured individuals. Eleven healthy and recreationally active participants (eight males, three females; age: 27 ± 7 years; body mass index: 23.5 ± 2.5 kg·m–2) volunteered to participate in three trials: HIIE (10 × 60 s at 80% peak power output), MICE (50% peak power output of isocaloric duration), and a no-exercise control condition. Each exercise bout was performed at 18:00, and participants consumed a standardised evening meal at 20:00. Following an overnight fast, a 5-h mixed-macronutrient tolerance test was performed at 08:00. There were no significant differences in triglyceride incremental area under the curve between HIIE (192 ± 94 mmol·L–1 per 300 min), MICE (184 ± 111 mmol·L–1 per 300 min), and the no-exercise condition (175 ± 90 mmol·L–1 per 300 min) ( P = 0.46). There were no significant differences in incremental area under the curve for glucose ( P = 0.91) or insulin ( P = 0.59) between conditions. Upper-body MICE and HIIE performed in the evening do not influence PPL the following morning, in normotriglyceridemic individuals. Clinical Trials Registration: NCT04277091. Novelty: Arm-crank exercise has no effect on PPL when performed the evening prior to a mixed-macronutrient meal test. Upper-body sprint interval exercise should be investigated as a potential solution to reduce PPL.
Effects of sex and wet-bulb globe temperature on heart rate variability during prolonged moderate-intensity exercise: a secondary analysis
Authors: De Barros Jordan A. , Macartney Michael J. , Peoples Gregory E. , Notley Sean R. , Herry Christophe L. , Kenny Glen P. 
Published: March 2022

Sex differences in heart rate (HR) and heart rate variability (HRV), a surrogate of cardiac autonomic modulation, are evident during rest and exercise in young healthy individuals. However, it remains unclear whether sex impacts HRV during prolonged exercise at differing levels of environmental heat stress. Therefore, we completed a secondary analysis upon the effects of sex and wet-bulb globe temperature (WBGT) on HR and HRV during prolonged exercise. To achieve this, HR and HRV were assessed in non-endurance-trained and non-heat-acclimatised healthy men ( n = 19) and women ( n = 15) aged 18–45 years during 180 min of treadmill walking at a moderate metabolic rate (200 W/m2: equivalent to ∼35% peak aerobic power) in 16, 24, 28, and 32 °C WBGT. In the final 5 min prior to exercise termination, HR was observed to be higher in women relative to men in all but the 32 °C WBGT. Although no sex differences were observed for the HRV metric of root-mean-square of successive differences, high-frequency power was higher in women relative to men across WBGT conditions. These findings indicate that, in healthy non-heat-acclimatised individuals, women respond to prolonged exercise-heat stress with a greater increase in HR despite cardiac vagal autonomic modulation remaining equal or increasing compared to men. Novelty: Prior to exercise termination, females respond with a greater increase in HR under all WBGTs except the hottest (32 °C). Sex influenced HRV metrics during all WBGTs, but results were mixed. Further characterisation of HRV sex differences remains an important area of research.
Isotopic and traits-based trophic diversity of Canadian Beaufort Sea benthic communities in relation to food supply
Authors: Ehrman Ashley D. , Swanson Heidi , MacPhee Shannon , Majewski Andrew , Archambault Philippe , Eert Jane , Reist James , Power Michael 
Published: March 2022

In the Arctic, climate-driven alterations to the quality and quantity of organic matter reaching the seafloor will likely affect benthic food web function. We used biomass-weighted diversity measures based on trophic traits (those related to feeding behaviours, e.g., mobility, size, food source), stable isotope ratios (δ15N and δ13C), and taxonomy to assess linkages between benthic food web structure and indicators of food supply in the shelf and slope ecosystems of the Canadian Beaufort Sea and Amundsen Gulf (down to 500 m). Benthic communities across both regions used a similar diversity of sedimentary organic matter sources according to stable isotopes, despite known differences between regions in organic matter input and benthic–pelagic coupling. Shelf-edge and upper-slope communities exhibited relatively high trophic trait diversity, which likely reflected the exploitation of pulsed food inputs associated with dynamic processes at the shelf break that affect the production and advection of organic matter to the benthos. Pairwise relationships between trophic traits and sedimentary proxies of benthic food supply were not significant at the regional scale. However, cluster analyses supported the notion that trophic trait composition was influenced by proximity to the shelf edge, where food supply is influenced by episodic events that may not be reflected by in situ sedimentary proxies. Our findings add to growing evidence that evaluating trophic traits composition may provide more information regarding functional responses to changes in benthic food supply than either isotopic or taxonomic diversity indices alone.
Modeling RSM of photocatalytic treatment of Acid Red 18 pollutant using ZnO–Cr nano-photocatalyst, kinetic studies, and energy management
Authors: Sharifi Abdolkarim , Rajabi Abhari Abbas , Imanzadeh Mehdi , Mahmoodi Zahra , Farrokhzadeh Samaneh 
Published: March 2022

The ZnO–Cr nano-photocatalyst was synthesized using a microwave-assisted solution combustion method and applied for the photodegradation of the organic pollutant Acid Red 18 (AR18). The synthesized nano-photocatalyst was characterized by XRD, FESEM, EDX, and FTIR methods. To reach the optimal condition of the treatment, the response surface methodology was used in the central composite design model. The amount of nano-photocatalyst, pH of the solution, and initial concentration of the pollutant were optimized. The polynomial 3-degree model was fitted to the photodegradation data, and the correlation coefficients of the model showed an interaction between the parameters. Optimization of the polynomial model for pollutant treatment was investigated under the same conditions, and the comparison of the observed and predicted treatment models showed a low difference in decolorization. The intermediates were identified by liquid chromatography/mass spectrometry. A kinetic study showed that the first-order kinetic constant for the degradation of pollutant concentrations from 10 to 30 mg L−1 changed from 0.0178 to 0.0058 min–1. Finally, economic evaluation and energy management of the process showed that the decolorization process was more economical at low pollutant concentrations.
Isotopic Age of the Xiong’er Group Volcanic Rocks and Its Geological Significance in Western Henan, China
Authors: Dong Wenchao , Pang Xucheng , Qu Weixun , Hou Guangshun 
Published: March 2022

The Xiong’er Group is mainly distributed in western Henan, southern Shanxi, and southeastern Shaanxi. As a set of low-grade metamorphic and low-deformation volcanic rocks formed in the transition period during the tectonic evolution of the North China Craton in the Precambrian, the tectonic and magmatic evolution information of this period are well recorded. The accurate isotopic dating of the Xiong’er Group has great significance to the study of the stratigraphic division and formation of volcanic rocks in the Xiong’er Group and the improved cognition of regional tectonic magmatism. In this study, through the SHRIMP (sensitive high-resolution ion microprobe) zircon uranium-lead (U-Pb) isotope dating of the volcanic rocks of the Xiong’er Group in the Xiong’er Mountain, the research results showed that the age data were separated into two intervals, the magmatic zircon ages from 1836 to 1711 Ma and the inherited zircon ages from

2415
±
19

to

2193
±
34

Ma

. The isotopic age of magmatic zircon was considered to represent the formation age of the Jidanping Formation in the Xiong’er Mountain, and the isotopic ages of the inherited zircons were consistent with the formation ages of the Taihua Group supracrustal rocks in the lower Xiong’er Group. Combined with previous studies on isotope and rock geochemistry of volcanic rocks in the Xiong’er Group, it was believed that the formation ages of the volcanic rocks of the Xiong’er Group are between 1874 and 1618 Ma, among the ages of the Xushan Formation, Jidanping Formation, and Majiahe Formation, which were concentrated between 1874 and 1800 Ma, 1836 and 1711 Ma, and 1780 and 1618 Ma, respectively. The age of inherited zircon in the volcanic rocks of the Xiong’er Group indicated that the formation of the volcanic rocks of the Xiong’er Group had the addition of stratigraphic materials from the Taihua Group.
A Novel Synthetic Lethal Approach to Target MYC-Driven Cancers
Authors: Chabanon Roman M. , Postel-Vinay Sophie 
Published: March 2022

The MYC proto-oncogene family encompasses three related transcription factors (MYC, MYCL, and MYCN), which are master regulators of cellular programs orchestrating multiple hallmarks of cancer, including proliferation, metabolism, invasiveness, and immune surveillance. MYC activation is one of the most frequent alterations in cancer, induced by genetic, epigenetic, or posttranslational alterations of MYC itself, or of MYC-related proteins or pathways. Sun and colleagues found a unique function of the rate-limiting nucleotide synthesis enzyme CTP synthase 1 (CTPS1) in the survival of MYC-driven cancer cells. They further identified a novel synthetic lethal strategy to combat MYC-driven cancers by combining CTPS1 inhibitors with ataxia telangiectasia and Rad3-related protein inhibitors, which exploits the inherent vulnerability of MYC-driven tumors to nucleotide shortage and DNA replication stress. These findings open novel therapeutic avenues for targeting the traditionally “undruggable” MYC-driven cancers, which represent one of the highest unmet clinical needs in cancer.
See related article by Sun et al. p. 1013
IDO Vaccine Ablates Immune-Suppressive Myeloid Populations and Enhances Antitumor Effects Independent of Tumor Cell IDO Status
Authors: Nandre Rahul , Verma Vivek , Gaur Pankaj , Patil Veerupaxagouda , Yang Xingdong , Ramlaoui Zainab , Shobaki Nour , Andersen Mads Hald , Pedersen Ayako Wakatsuki , Zocca Mai-Britt , Mkrtichyan Mikayel , Gupta Seema , Khleif Samir N. 
Published: March 2022

Abstract

The immunosuppressive tumor microenvironment (TME) does not allow generation and expansion of antitumor effector cells. One of the potent immunosuppressive factors present in the TME is the indoleamine-pyrrole 2,3-dioxygenase (IDO) enzyme, produced mainly by cancer cells and suppressive immune cells of myeloid origin. In fact, IDO+ myeloid-derived suppressor cells (MDSC) and dendritic cells (DC) tend to be more suppressive than their IDO− counterparts. Hence, therapeutic approaches that would target the IDO+ cells in the TME, while sparing the antigen-presenting functions of IDO− myeloid populations, are needed. Using an IDO-specific peptide vaccine (IDO vaccine), we explored the possibility of generating effector cells against IDO and non-IDO tumor-derived antigens. For this, IDO-secreting (B16F10 melanoma) and non–IDO-secreting (TC-1) mouse tumor models were employed. We showed that the IDO vaccine significantly reduced tumor growth and enhanced survival of mice in both the tumor models, which associated with a robust induction of IDO-specific effector cells in the TME. The IDO vaccine significantly enhanced the antitumor efficacy of non-IDO tumor antigen–specific vaccines, leading to an increase in the number of total and antigen-specific activated CD8+ T cells (IFNγ+ and granzyme B+). Treatment with the IDO vaccine significantly reduced the numbers of IDO+ MDSCs and DCs, and immunosuppressive regulatory T cells in both tumor models, resulting in enhanced therapeutic ratios. Together, we showed that vaccination against IDO is a promising therapeutic option for both IDO-producing and non–IDO-producing tumors. The IDO vaccine selectively ablates the IDO+ compartment in the TME, leading to a significant enhancement of the immune responses against other tumor antigen–specific vaccines.
Heritability of Coronary Artery Disease: Insights From a Classical Twin Study
Authors: Drobni Zsofia D. , Kolossvary Marton , Karady Julia , Jermendy Adam L. , Tarnoki Adam D. , Tarnoki David L. , Simon Judit , Szilveszter Balint , Littvay Levente , Voros Szilard , Jermendy Gyorgy , Merkely Bela , Maurovich-Horvat Pal 
Published: March 2022

Background:
Genetics have a strong influence on calcified atherosclerotic plaques; however, data regarding the heritability of noncalcified plaque volume are scarce. We aimed to evaluate genetic versus environmental influences on calcium (coronary artery calcification) score, noncalcified and calcified plaque volumes by coronary computed tomography angiography in adult twin pairs without known coronary artery disease.

Methods:
In the prospective BUDAPEST-GLOBAL (Burden of Atherosclerotic Plaques Study in Twins—Genetic Loci and the Burden of Atherosclerotic Lesions) classical twin study, we analyzed twin pairs without known coronary artery disease. All twins underwent coronary computed tomography angiography to assess coronary atherosclerotic plaque volumes. Structural equation models were used to quantify the contribution of additive genetic, common environmental, and unique environmental components to plaque volumes adjusted for age, gender, or atherosclerotic cardiovascular disease risk estimate and statin use.

Results:
We included 196 twins (mean age±SD, 56±9 years, 63.3% females), 120 monozygotic and 76 same-gender dizygotic pairs. Using structural equation models, noncalcified plaque volume was predominantly determined by environmental factors (common environment, 63% [95% CI, 56%–67%], unique environment, 37% [95% CI, 33%–44%]), while coronary artery calcification score and calcified plaque volumes had a relatively strong genetic heritability (additive genetic, 58% [95% CI, 50%–66%]; unique environmental, 42% [95% CI, 34%–50%] and additive genetic, 78% [95% CI, 73%–80%]; unique environmental, 22% [95% CI, 20%–27%]), respectively.

Conclusions:
Noncalcified plaque volume is mainly influenced by shared environmental factors, whereas coronary artery calcification score and calcified plaque volume are more determined by genetics. These findings emphasize the importance of early lifestyle interventions in preventing coronary plaque formation.

Registration:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT01738828.