bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Investigation Into the Conjunctival and Nasal Flora of Patients Receiving Cyclosporine Eye Drop Treatment for Dry Eye
Authors: elibol Emine Savran , habip Zafer , Elbay Ahmet , Cırık Ahmet adnan , Oğuz Halit 
Published: March 2022

Abstract
OBJECTIVEThe aim of this study is to analyze the long-term use of cyclosporine drops of anti-inflammatory effect on conjunctival and nasal flora, which are commonly used in dry eye treatment, , to examine antibiotic resistance and to suggest some precautions.METHODSThe 38 people using cyclosporine drops were classified as Group 1, and the 34 people using preservative-free artificial tear preparations were classified as Group 2. Swabs were taken from the conjunctiva and nasal cavities of the volunteers participating in the study and cultured. Groups 1 and 2 were compared in terms of growing microorganisms and antibiotic susceptibility.RESULTSThere was no statistically significant difference between Groups 1 and 2 in terms of bacteria growing in the eye and nasal cavities. When it comes to the antibiotic susceptibility statistics for S. epidermidis, low sensitivity to penicillin was observed in particular in both groups, but there was no significant difference between the two groups (p=0.036)CONCLUSIONThe use of cyclosporine or preservative-free artificial tears did not make any difference to the flora or to the antibiotic susceptibility of the flora. It is thought that the use of cyclosporine drops does not predispose to ocular infection.
Aptasensor Based on Screen-Printed Carbon Electrodes Modified with Aunps/CS for Sensitive Detection of Okadaic Acid in Shellfish
Authors: Dong Yiyang , Zeng Ni , Yang Yan , Yin Yingai , Zhao Lianhui , Wang Xu 
Published: March 2022

Abstract
Objective
Based on the specific binding of target with aptamer, a portable aptasensor combined with highly sensitive electrochemical methods was designed to complete the rapid detection of biological toxins Okadaic acid (OA) in shellfish.
Results
A simple aptasensor based on screen-printed carbon electrode (SPCE) with modification of chitosan (CS) and gold nanoparticles (AuNPs) was designed for electrochemical determination of OA, and the electrode surface was modified with AuNPs by potential-sweeping electrodeposition, which greatly improved the electrochemical response. The entire detection and characterization process were carried out by cyclic voltammetry (CV) with a linear correlation in the range from 0.01 ng/mL to 100 ng/mL and a LOD of 6.7 pg/mL. Furthermore, a recovery rate of 92.30-115.98% was obtained demonstrating excellent accuracy through the recovery trial of mussel and scallop samples.
Conclusion
The current method based the change of spatial structure before and after aptamer binding target showed great potential in rapid on-site and low-cost detection of OA for its simplicity.
Posterior Tibial Slope and Medial Tibial Depth Measured by MRI in Patients with Posterior Cruciate Ligament Injury: A Case-Control Study
Authors: Zhou Aiguo , Yin Baoshan , Zhao Pei , Chen Jiaxing , Yan Wenlong , Zhang Hua , Zhang Jian 
Published: March 2022

Abstract
Objectives To 1) figure out the difference in tibial morphologies between patients with posterior cruciate ligament (PCL) injury and those without, 2) identify any tibial anatomical variations contributing to PCL rupture.Design Retrospective case-control study, level of evidence III.Methods 45 patients with PCL rupture from 2015 to 2020 in our hospital, and 45 patients matched by age and sex with intact PCL were enrolled in our study. The intraclass correlation coefficient (ICC) was used to assess the reliability of each parameter. The independent t-test was conducted to identify the differences in tibial morphometric characteristics between the PCL-injured and PCL-intact individuals, including the posterior tibial slope (PTS), meniscal slope (MS), medial tibial depth (MTD). A binary logistic regression model was established to evaluate the roles of those anatomic parameters of interest play in PCL injuries. Results The interobserver reliability of each parameter showed excellent agreement. Significant differences in the medial (P= .023) and lateral (P= .009) PTS were found between the PCL-injured group (3.68 ± 2.70 and 4.55 ± 3.19, respectively) and the controls (5.00 ± 2.73 and 6.39 ± 3.29, respectively). And the MTD was 1.98 ± 0.64 mm in the PCL-injured group and 2.37 ± 0.55 mm in the control group (P=0.007). Binary logistic regression analysis showed that smaller lateral PTS and MTD were directly associated with PCL injury, with an OR of 1.17 and OR of 3.14, respectively. The medial PTS was independent to PCL injures.Conclusion In patients with PCL injury, lateral PTS, medial PTS, and MTD were found smaller compared to PCL intact controls. Decreased lateral PTS and MTD were underlying anatomic risk factors for PCL injury, with each unit reduction, the risks of PCL injury were 1.2 and 3.1-fold higher, respectively.
Elongated soy protein isolate-poly(D, L-lactide-co-glycolic acid) microcapsules prepared using syringe filters and their effect on self-healing efficiency of soy protein-based green resin
Authors: Shi Shanshan , Netravali Anil 
Published: March 2022

Abstract
A self-healing soy-based resin filled with soy protein isolate (SPI) containing poly(D, L-lactide-co-glycolic acid) (PLGA) microcapsules (MCs) was prepared. MCs were prepared using a syringe filter via a membrane emulsification technique. Using this method, spherical (diameter of about 1.30 μm) and elongated MCs (aspect ratios up to 20) could be produced. Tensile properties and self-healing efficiencies of resins were characterized for both MC morphologies in terms of strength recovery and toughness recovery. While both MCs could successfully heal cracks, spherical MCs were seen to compromise the tensile properties of the resin, whereas elongated MCs demonstrated some mechanical enhancement. Overall results showed that elongated MCs having a higher aspect ratio resulted in slightly better self-healing performance as well as a mechanical improvement. The results also show that higher MC loading results in higher self-healing efficiency.
The flax genome reveals orbitide diversity
Authors: Song Ziliang , Burbridge Connor , Schneider David , Sharbel Timothy , Reaney Martin 
Published: March 2022

Abstract
Background: Ribosomally-synthesized cyclic peptides are widely found in plants and show useful bioactivities for humans. The identification of cyclic peptide sequences and their precursor proteins is facilitated by the growing number of sequenced genomes. While previous research largely focused on the chemical diversity of these peptides across various species, there is little attention to a broader range of potential peptides that are not chemically identified. Results: A pioneering study was initiated to explore the genetic diversity of linusorbs, a group of cyclic peptides uniquely occurring in cultivated flax (Linum usitatissimum). Phylogenetic analysis categorized the 5 known linusorb precursor proteins into two clades and one singleton. Preliminary tBLASTn search of the published flax genome using the whole protein sequence as query could only retrieve its homologues within the same clade. This limitation was overcome using a profile-based mining strategy. After genome reannotation, a hidden Markov Model (HMM)-based approach identified 58 repeats homologous to the linusorb-embedded repeats in 8 novel proteins, meaning that they likely share common ancestry with the linusorb-embedded repeats. Subsequently, we developed a customized profile composed of a random linusorb-like domain (LLD) flanked by 5 conserved sites and used it for string search of the proteome, which extracted 281 LLD-containing repeats (LLDRs) in 25 proteins. Comparative analysis of different repeat categories suggested that the 5 conserved flanking sites among the non-homologous repeats have undergone convergent evolution driven by functional selection. Conclusions: The profile-based mining approach is suitable for analyzing repetitive sequences. The 25 LLDR proteins identified herein represent the potential diversity of cyclic peptides within the flax genome and lay a foundation for further studies on the functions and evolution of these protein tandem repeats.
Five-Year Stability Of Posterior Corneal Surface After Small Incision Lenticule Extraction For High Myopia
Authors: Zhao Yu , Lin Xue , Chen Zhuoyi , Zhou Xingtao 
Published: March 2022

Abstract
Background: To study the 5-year changes in the posterior corneal surface after small incision lenticule extraction (SMILE) for high myopia.Methods: Eighty eyes received SMILE was included in this prospective study. They were allocated into two groups based on the spherical equivalent: high myopia (40eyes, -7.49±0.70D) and moderate myopia (40eyes, -4.43±0.87D). Certain points of posterior corneal elevation (the central point (PCE), thinnest point (PTE), maximal point (PME), and in various corneal areas) were evaluated using a Scheimpflug camera (Pentacam; Oculus GmbH, Germany) preoperatively and at 6 months and 5 years after surgery. Results: All surgeries were completed uneventfully and no ectasia was developed throng the observation. The safety index and efficacy index were 1.14 and 1.03 in the high myopia group, and 1.16 and 1.06 in the moderate myopia group, respectively. Most of the calculated values in the high myopia group showed a slight increase at 6 months but decreased at 5 years. At 5 years postoperatively, the value of the PTE was significantly lower than at baseline in both groups (P≤0.047); a statistical difference was also revealed in the PME in the moderate group with slight changes (10.15±3.01μm vs. 11.60±4.33μm, P=0.002); no statistical significance was observed in other calculated values (P≥0.067). Similarly, no significant linear correlation was noted between changes in all values and the residual bed thickness either (P≥0.057).Conclusions: SMILE causes no protrusion in posterior corneal surface for correction of high myopia at the follow-up visit of 5 years.
Prevalence and associated factors for pterygium in a Chinese rural population with type 2 diabetes in a cross-sectional study: Jiangsu Diabetic Eye Disease Study (JDEDS)
Authors: Zhang Junfang , Qin Bai , Liu Bihong , Sun Dajun , Liang Congkai , Wang Shiyi , Yang Mei , Zhu Rongrong 
Published: March 2022

Abstract
Purpose: To investigate the pterygium prevalence and evaluate risk factors of pterygium in rural community-based type 2 diabetic (D2M) patients aged 50 years and above in Funing Country, Jiangsu Province, China. Methods A cross-sectional ophthalmic survey was conducted in type 2 diabetes mellitus (D2M) patients aged ≥50 years in Funing county, Jiangsu Province, China, which was named Jiangsu Diabetic Eye Disease Study (JDEDS). All participants underwent a comprehensive questionnaire and ocular examination. Pterygium was diagnosed by slit lamp examination. The risk factors associated with pterygium were evaluated with logistic regression models.Results The prevalence of pterygium was 22.37% (n= 427) and 95% confidence interval (CI): (20.50-24.24%) in D2M patients aged 50 years and above in JDEDS. The prevalence of pterygium was 18.32% (95% CI: 15.33~21.32%) in men and 24.43% (95% CI: 22.06~26.80%) in women. Women had a higher prevalence than men (p= 0.001). Multivariate analysis showed, for male participants with D2M, pterygium was independently associated with increasing age [ 70-79yrs: OR and 95% CI: 2.49(1.20~5.18), p=0.014; ≥80yrs: 4.84(2.04~11.47), p<0.001], while cigarette smoking was the protective factors, especially in current smoker [OR and 95% CI: 0.79(0.67~0.92); p=0.003]. For female participants with D2M, age [ 60-69yrs OR and 95% CI: 1.68(1.07~2.62), p=0.023; 70-79yrs: 2.62(1.69~4.06), p<0.001; ≥80yrs:3.24(1.70~5.90), p<0.001], hypertension [OR and 95%CI: 1.40(1.05~1.87), p=0.024], BMI 24-27.9 [OR and 95%CI: 1.20(1.00~1.44), p=0.047], higher HbA1c [ (5.6-7.9) % OR and 95%CI: 1.42(1.10~1.82), p=0.006; (8.0-9.9) %: 1.32(1.10~1.58), p=0.003] were risk factors. Conclusions D2M patients aged over 50 years has a high prevalence of pterygium in JDEDS. The pterygium prevalence is higher in female D2M participants. Diabetes and related factors may be risk factors of pterygium in female D2M patients. Further studies are needed to explore the gender difference in the pathogenesis of pterygium in D2M subjects.
LncRNA NDRG1 aggravates progression and EMT of osteosarcoma by sponging miR-96-5p through the PI3K/AKT pathway
Authors: Wang Zhen , Zhu Jie , Han Qixiu , Yu Lingfeng , Liu Ziying , Huang Jianhao , Zhu Yan , Zhu Hao , Fan Gentao , Tang Qikai , Qian Ji , Zhou Guangxin 
Published: March 2022

Abstract
Background
Osteosarcoma (OS) is the most common primary malignant bone tumors in adolescents. Large numbers of studies have focused on the long non-coding RNA (lncRNA) that plays essential roles in the progression of osteosarcoma. Nevertheless, the functions and underlying mechanisms of LncRNA NDRG1 in osteosarcoma remain unknown.
Methods
By ways of RNA sequencing, lncRNAs which expressed differentially between osteosarcoma and adjacent tissues were identified. Through in vitro and in vivo functional experiments, the effect of LncRNA NDRG1 in osteosarcoma growth and metastasis were investigated. LncRNA NDRG1 specific binding with miR-96-5p was verified through bioinformatic analysis and luciferase reporter assay. Regulation relationship between Lnc NDRG1 and miR-96-5p was further evaluated by the rescue experiments. Additionally, the changes in the expression of epithelial-mesenchymal transition (EMT) and the PI3K/AKT pathway molecular markers were verified by Western blot.
Results
LncRNA NDRG1 was up-regulated in osteosarcoma cell lines and tissues and the expression of LncRNA NDRG1 was correlated with the overall survival of osteosarcoma patients. LncRNA NDRG1 aggravated the progression of osteosarcoma both in vitro and in vivo. Mechanistically, LncRNA NDRG1 was found to promote progression and epithelial-to-mesenchymal transition (EMT) of osteosarcoma by sponging miR-96-5p through the PI3K/AKT pathway.
Conclusions
LncRNA NDRG1 aggravates the proliferation, metastasis and EMT of osteosarcoma by sponging miR-96-5p through the PI3K/AKT pathway. Therefore, LncRNA NDRG1 could act as a prognostic marker and a therapeutic target for osteosarcoma in the future.
Global assessment of three Rumex species reveals inconsistent climatic niche shifts across introduced ranges
Authors: Carlin Thomas , Bufford Jennifer L , Hulme Philip E , Godsoe William K 
Published: March 2022

Abstract
Climatic niche shifts occur when species occupy different climates in the introduced range than in their native range. Climatic niche shifts are known to occur across a range of taxa, however we do not currently understand whether climatic niche shifts can consistently be predicted across multiple introduced ranges. Using three congeneric weed species, we investigate whether climatic niche shifts in one introduced range are consistent in other ranges where the species has been introduced. We compared the climatic conditions occupied by Rumex conglomeratus , R. crispus , and R. obtusifolius between their native range (Eurasia) and three different introduced ranges (North America, Australia, New Zealand). We considered metrics of niche overlap, expansion, unfilling, pioneering, and similarity to determine whether climatic niche shifts were consistent across ranges and congeners. We found that the presence and direction of climatic niche shifts was inconsistent between introduced ranges for each species. Within an introduced range, however, niche shifts were qualitatively similar among species. North America and New Zealand experienced diverging niche expansion into drier and wetter climates respectively, whilst the niche was conserved in Australia. This work highlights how unique characteristics of an introduced range and local introduction history can drive different niche shifts, and that comparisons between only the native and one introduced range may misrepresent a species’ capacity for niche shifts. However, predictions of climatic niche shifts could be improved by comparing related species in the introduced range rather than relying on the occupied environments of the native range.
Mitochondrial transfer restores the proliferation of injured endometrial stromal cell
Authors: Chen Liang , Zhu Jue , Chen Yicheng , Zhu Miaohua , Wang Qiming , Zhang Jing 
Published: March 2022

Abstract
Background: The uterine endometrium is a tissue that needs sufficient energy to support its active physiological function, the damage of which is responsible for intrauterine adhesion. In this study, we investigated the evidence that mitochondrial transfer is involved in endometrial repair and explored the effects and possible mechanism of mitochondrial transfer.Methods: The study was approved by the regional ethics committee of Ningbo Women and Children’s Hospital (Date: 12/3/2021/No: EC2021-M004). Endometrial stromal cells were pretreated with hypoxia and cocultured with human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) in vitro. Confocal imaging was used to assess mitochondrial transfer between the cells. The proliferation and ATP production of endometrial stromal cells were detected to evaluate the physiological function of the cells.Results: We found that coculture of hUCB-MSCs and endometrial stromal cells partially restored the proliferation and ATP production of hypoxic cells. Mitochondrial transfer was observed from hUCB-MSCs to hypoxic endometrial stromal cells. This process can be blocked by hUCB-MSCs pretreated with carbenoxolone disodium (CBX), which can block TNT connection formation.Conclusions: The mitochondria of stem cells could be transferred completely into hypoxic endometrial stromal cells, partially restoring the proliferation of injured cells in vitro. This process is proactive.Trial registration: The study was approved by the regional ethics committee of the Ningbo women and Children’s Hospital (Date: 12/3/2021/No: EC2021-M004).
Enrolling People of Color to Evaluate a Practice Intervention: Lessons from the Shared Decision-Making for Atrial Fibrillation (SDM4AFib) Trial
Authors: Sivly Angela , Gorr Haeshik S. , Gravholt Derek , Branda Megan E. , Linzer Mark , Noseworthy Peter , Hargraves Ian , Kunneman Marleen , Doubeni Chyke A. , Suzuki Takeki , Brito Juan P. , Jackson Elizabeth A. , Burnett Bruce , Wambua Mike , Montori Victor M. 
Published: March 2022

Abstract
Background: Trial recruitment of Black, indigenous, and people of color (BIPOC) is key for interventions that interact with socioeconomic factors and cultural norms, preferences, and values. We report on our experience enrolling BIPOC participants into a multicenter trial of a shared decision-making intervention about anticoagulation to prevent strokes, in patients with atrial fibrillation (AF). Methods: We enrolled patients with AF and their clinicians in 5 healthcare systems (three academic medical centers, an urban/suburban community medical center, and a safety-net inner-city medical center) located in three states (Minnesota, Alabama, and Mississippi) in the United States. Clinical encounters were randomized to usual care with or without a shared decision-making tool about anticoagulation. Analysis: We analyzed BIPOC patient enrollment by site, categorized reasons for non-enrollment, and examined how enrollment of BIPOC patients was promoted across sites.Results: Of 2247 patients assessed, 922 were enrolled of which 147 (16%) were BIPOC patients. In comparison to trial-eligible White participants, eligible Black participants more frequently did not enroll (102 (11%) vs. 185 (15%); p <.001). The rate of BIPOC patients enrollment varied substantially by site and was generally reflective of the population served at each participating site. Enrollment of BIPOC patients benefited most from including and prioritizing practices most likely to care for BIPOC patients, with lower yield from specific efforts to reach BIPOC clinic attendees and enrolling BIPOC patients first when possible. Conclusions: Enrollment from practices that have implemented policies that favor the care of BIPOC patients can contribute to generate trustworthy estimates of the effect of these interventions in clinical practice that are applicable to BIPOC populations. Best practices to optimize the enrollment of BIPOC participants into trials that examined complex and culturally sensitive interventions remain to be developed.Clinical trial registration: ClinicalTrials.gov (NCT02905032)
Calcium Peroxide Alleviates the Waterlogging Stress of Rapeseed by Improving the Rhizosphere Oxygen Environment in a Rice-rape Rotation Field
Authors: Wang Zhiyuan , Han Yongliang , Luo Shang , Rong Xiangmin , Song Haixing , Jiang Na , Yang Lan 
Published: March 2022

Abstract
Purpose
Waterlogging stress has a negative influence on agricultural production, particularly for rapeseed yield in a rice-rape rotation field. Calcium peroxide reacts with water to release oxygen, which can serve as an excellent supply.
Methods
In this field experiment, the conventional rape (Brassica napus L.) variety fengyou958 (FY958) and the early maturing rape variety xiangyou420 (XY420) were used to evaluate the effects of calcium peroxide on waterlogging resistance in a rice-rape rotation field. Waterlogging was imposed from the seedling to flowering stages, and three concentrations of calcium peroxide (0, C1 for the 0.594 kg plot− 1 and C2 for the 0.864 kg plot− 1) were added as basal fertilizer.
Results
Waterlogging decreased the yield of XY420 and FY958 rapeseed by 74.8% and 87.4%, respectively, while the leaf SPAD values decreased significantly by 17.2% and 21.5%. Treatment with calcium peroxide resulted in an increase of 151%-293% in the yield of rapeseed compared with the waterlogged plants. Moreover, calcium peroxide alleviated the peroxidation of leaves and decreased the activities of catalase and superoxide dismutase by 14.2%-44.4% and 10.9%-19.5%. Compared with the waterlogged plants, the contents of abscisic acid and ethylene in the C2 leaves decreased by 37.3% and 33.4%, while the content of gibberellin increased significantly by 44.9%. The belowground parts of the plants were also significantly affected. The treatments with calcium peroxide reduced the activities of fermentation enzymes in the roots, which significantly reduced the accumulation of fermentation products by 25.0%-57.1% and 16.5%-29.4% for lactate and ethanol, respectively. This reduction was owing to the improved levels of oxygen in the root environment that were released by calcium peroxide.
Conclusions
The application of a suitable concentration of calcium peroxide enhanced the resistance of rapeseed to waterlogging stress, and this study provides a valid method to prevent damage from flooding in rice-rape rotation fields.
The biodegradation behavior of an eco-characteristic Bio-formulated polymer nanosheets and eco-efficacy assessment for promoting growth of Eggplant (Solanum melongena L.)
Authors: Mary T. Ruby Nirmala , Ramasamy Jayavel 
Published: March 2022

Abstract
The introduction of Cashew Nut Shell Liquid (CNSL), iron oxide nanoparticles, Plant extracts from Mentha spicata L., Vitex negundo L., and Eucalyptus globulus M., in chitosan biopolymer matrix has created a significant contribution for the development of Bio-formulated polymer nanosheets. The migration test performed for the formulated Bio-nanosheets have satisfactory results with very less amount of residue get migrated that revealed a suitable material for packaging application. The developed bio-formulated polymer nanosheets achieved an excellent biodegradation activity of about 80-93% through the acceleration of soil microbial flora. The SEM and EDAX analysis confirmed the surface degradability variation observed for 5 weeks. The present study is focused on the application of biodegraded soil for promoting the Eggplant growth (Solanum melongena L.) and its eco-efficacy evaluated by enhancing the development of an eco-characteristic active bio-nanomaterials which resulted a positive impact in packaging and agricultural sectors.