bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evoked responses to single pulse electrical stimulation demonstrate impaired striatal excitability in a rodent model of Parkinson’s disease
Authors: Gronlier Eloïse , Volle Julien , Coizet Véronique , Antoine Paccard , Habermacher Chloé , Roche Yann , Roucard Corinne , Duveau Venceslas , David Olivier 
Published: November 2022

Abstract
Background: Sensorimotor beta oscillations are increased in Parkinson’s disease (PD) due to the alteration of dopaminergic transmission. This electrophysiological read-out is reported both in patients and in animal models such as the 6-OHDA rat model obtained with unilateral nigral injection of 6-hydroxydopamine (6-OHDA). Current treatments, based on dopaminergic replacement, transiently normalize this pathological beta activity and improve patients’ quality of life.
Objectives: We wanted to assess in vivo whether the abnormal beta oscillations can be directly linked to the striato-cortical excitability of the sensorimotor system and whether it is modulated by the pharmacological manipulation of the dopaminergic system.
Methods: In the unilateral 6-OHDA rat model and control animals, we used intra-striatal and intra-cortical single-pulse electrical stimulation (SPES) and concurrent local field potentials (LFP) recordings. In the two groups, we quantified basal cortico-striatal excitability from time-resolved spectral analyses of LFP evoked responses induced remotely by intracerebral stimulations. The temporal dependance of cortico-striatal excitability to dopaminergic transmission was further tested using electrophysiological recordings combined with levodopa injection.
Results: LFP evoked responses after striatal stimulation showed a transient reduction of power in a large time-frequency domain in the 6-OHDA group compared to the sham group. This observation is specific to the striatum, as no significant difference was observed in cortical LFP evoked responses between the two groups, and corresponds to an impaired striatal excitability in the 6-OHDA group compared to the controls. This PD-specific effect was observed in the striatum at least during the first 3 months after the lesion. In addition, the striatum responses to SPES during a levodopa challenge showed a transient potentiation of the decrease of responsiveness in frequencies below 40 Hz.
Conclusion: The spectral properties of striatal responses to SPES show high sensitivity to dopaminergic transmission in the unilateral 6-OHDA rat model. We thus propose that this approach could be used in preclinical models as a time-resolved biomarker of impaired dopaminergic transmission capable of monitoring progressive neurodegeneration and/or challenges to drug intake.
Arbuscular mycorrhiza spore abundance and species richness in main agroecosystems in five regions in southeast Mexico
Authors: Ricárdez-Pérez Juan David , Wal Hans van der 
Published: November 2022

Abstract
Geographical setting and agroecosystem management have been found to influence spore abundance and species composition of Arbuscular Mycorrhizal Fungi (AMF) communities. In this study we determine how abundance and composition vary between five regions in Southeast México and between the main agroecosystems within them. Two-way analysis of variance and post-hoc Tukey-tests of spore abundance data of home gardens, mixed maize crops and pasturelands showed higher abundances in Los Altos and Canón Usumacinta than in Los Ríos, a significant region–agroecosystem interaction and no effect of agroecosystem. Analysis by region showed significant differences between agroecosystems in the Chontalpa and the Cañón Usumacinta (Kruskal-Wallis and Mann-Whitney tests), but not in Los Altos, Los Ríos and Southern Quintana Roo. We observed a total of 34 morphospecies from a total of 90 sampling sites and calculated an estimated total richness of 42.7 morphospecies. Estimated richness was higher in Los Rios (31.2.) and Chontalpa (19.1) than in southern Quintana Roo (9.6) and was intermediate in Los Altos (10.4) and Cañón Usumacinta (13.5). Species composition did not vary strongly between regions, as most species occurred in several regions. High spore abundance was associated with the moderate use of agrochemicals and of mechanized cropping practices.
Gut microbial profile of treatment-naive patients with primary biliary cholangitis
Authors: Zhou Yi-jun , Ying Gao-xiang , Dong Shi-lei , Xiang Bo , Jin Qiao-fei 
Published: November 2022

Abstract
Background and aims:
The pathogenesis of primary biliary cholangitis (PBC) is associated with alterations of gut microbiota. We compared the gut microbiota of PBC patients and healthy controls from Zhejiang province, and assessed the use of gut microbiota composition as a tool for diagnosis of PBC.
Methods
First, 16S rRNA gene sequencing was used to characterize the gut microbiota of patients with treatment-naive PBC (n = 25) and matched healthy controls (n = 25). Then, the use of gut microbiota composition for the diagnosis of PBC and as an indicator of PBC severity was determined.
Results
The gut microbiota of PBC patients had lower in three metric of alpha-diversity (ace, Chao1, and observed features) and fewer overall number of genera (all p < 0.01). PBC patients had significantly enrichment of 4 gut genera and significant depletion of 8 gut genera. We identified 6 amplicon sequence variants (Serratia, Oscillospirales, Ruminococcaceae, Faecalibacterium, Sutterellaceae, and Coprococcus) as optimal biomarkers to distinguish PBC patients from controls, based on receiver operating characteristic analysis which indicated the area under the curve was 0.824. PBC patients who were anti-gp210-positive had lower levels of Oscillospiraceae than those who were anti-gp210-negative. KEGG functional annotation suggested the major changes in the gut microbiota of PBC patients were related to lipid metabolism and biosynthesis of secondary metabolites.
Conclusion
We characterized the gut microbiota of treatment-naive PBC patients from Zhejiang province. These patients had significant alterations in their gut microbiota, suggesting a potential use of gut microbiota as a non-invasive tool for the diagnosis of PBC.
Subsoiling tillage enhances wheat productivity, soil carbon and available nutrients status in dryland fields
Authors: Yan Qiuyan , Wu Linjia , Dong Fei , Yan Shuangdui , Zhang Qiang , Li Xun , Li Junhui , Lu Jinxiu , Yang Feng , Jia Yaqin , Shen Yanting 
Published: November 2022

Abstract

Amis Additional tillage practices such as subsoiling (SS) and deep tillage (DT) during fallow period are beneficial to water storage and yield increases in dryland wheat fields compared with no tillage (NT). However, soil chemical characteristics responses to tillage practices that change within growth seasons are less understood. Methods This study evaluated the effects of three tillage practices (NT, SS and DT) on soil organic carbon (SOC), total nitrogen (TN), available nutrient and its relations with yield. Soil samples from five experimental years were analyzed to determine the contents of SOC, TN, and available nutrients. Soil samples from the fifth experimental year were analyzed to determine the soil aggregate and aggregate-associated C and N fractions. Results The SS and DP treatments favored grain yield, straw yield and straw carbon return compared to NT. In the whole profile (0–40 cm), the mean annual SOC stock and sequestration rate were 62.3, 59.4 and 38.5 t ha− 1and + 0.73, + 0.59 and − 8.27 t ha− 1yr− 1under NT, SS and DT. The mean annual TN stock and sequestration rate were 5.45, 6.18 and 5.95 t ha− 1and + 0.28, + 0.53 and + 0.67 t ha− 1yr− 1under NT, SS and DT. Higher SOC levels under SS and NT were associated with greater aggregate-associated C fractions. Similarly, soil available nutrients improved under NT and SS at 0–20 cm soil layer. Conclusions This result indicated that SS was an excellent practice for increasing wheat productivity, SOC, TN and nutrient availability synergistically in dryland wheat fields in North China.
Targeting the inward rectifier potassium channel 5.1 in thyroid cancer: artificial intelligence-facilitated molecular docking for drug discovery
Authors: YANG XUE , Du Yaying , Wu Yonglin , Xu Shaojie , Li Hanning , Peng Chengcheng , Cui Xiaoqing , Dhoomun Deenraj Kush , Wang Ge , Xu Tao , Dong Menglu , Li Xingrui 
Published: November 2022

Abstract
Purpose: Recurrent and metastatic thyroid cancer is more invasive and can transform to dedifferentiated thyroid cancer, thus leading to a severe decline in the 10-year survival. The thyroid-stimulating hormone receptor (TSHR) plays an important role in differentiation process. We aim to find a therapeutic target in redifferentiation strategies for thyroid cancer.
Methods: Our study integrated the differentially expressed genes acquired from the Gene Expression Omnibus database by comparing TSHR expression levels in the Cancer Genome Atlas database. We conducted functional enrichment analysis and verified the expression of these genes by RT-PCR in 68 pairs of thyroid tumor and paratumor tissues. Artificial intelligence-enabled virtual screening was combined with the VirtualFlow platform for deep docking.
Results: We identified five genes (KCNJ16, SLC26A4, T.G., TPO, and SYT1) as potential cancer treatment targets. TSHR and KCNJ16 were downregulated in the thyroid tumor tissues, compared with paired normal tissues. In addition, KCNJ16 was lower in the vascular/capsular invasion group. Enrichment analyses revealed that KCNJ16 may play a significant role in cell growth and differentiation. The inward rectifier potassium channel 5.1 (Kir5.1, encoded by KCNJ16) emerged as an interesting target in thyroid cancer. Artificial intelligence-facilitated molecular docking identified Z2087256678_2, Z2211139111_1, Z2211139111_2, and PV-000592319198_1 (-7.3 kcal/mol) as the most potent commercially available molecular targeting Kir5.1.
Conclusion: This study may provide greater insights into the differentiation features associated with TSHR expression in thyroid cancer, and Kir5.1 may be a potential therapeutic target in the redifferentiation strategies for recurrent and metastatic thyroid cancer.
The monocyte to high-density lipoprotein cholesterol ratio is positively associated with the severity of coronary artery disease in patients with nonalcoholic fatty liver disease
Authors: Zhang Yao , Wang Leigang , Zhao Jianqi , Pang Lin , Fan Hongxuan , Zhang Wenjing , Liang Bin 
Published: November 2022

Abstract
Background
Non-alcoholic fatty liver disease (NAFLD) is an independent risk factor for cardiovascular disease. Inflammation and dyslipidemia have been demonstrated to show excellent influence in atherosclerosis in patients with NAFLD. Monocyte (MON) and high-density lipoprotein cholesterol (HDL-C) ratio (MHR), one of the inflammation markers, is associated with the occurrence and severity of NAFLD. However, evidence of MHR in the risk of coronary artery disease (CAD) among NAFLD patients is limited.
Methods
We conducted a retrospective study of 612 patients with ultrasonogram-confirmed NAFLD who underwent percutaneous coronary intervention (PCI). All patients were divided into NAFLD + CAD group and NAFLD + non-CAD group. For univariate analysis between the two groups, continuous variables were compared using independent samples t-test or Mann-Whitney test, and categorical variables were compared using chi-square test or Fisher Exact tests. In addition, we used binary logistic regression analysis to identify risk and protective factors associated with CAD, constructed receiver operating characteristic (ROC) curves to assess the predictive value of MHR for CAD, and methodological studies of chi-square test for trend analysis the distribution of Gensini scores in MHR at different levels, and the risk degree of Gensini scores at different MHR levels by ordinal logistic regression.
Results
The study included a total of 336 CAD patients and 277 non-CAD patients. Univariate analysis showed that CAD patients had higher MHR than non-CAD patients (P < 0.001). Meanwhile, compared with pure NAFLD patients, multivariate logistic regression analysis showed that MHR remained to be an independent risk factor for CAD after adjusting for confounding risk factors (OR = 15.57, 95% CI 4.68–51.85, P < 0.001). At the same time, it was superior to traditional lipid parameters. NHR, NLR, and TG were also independent risk factors for CAD. HDL-C is a protective factor for CAD among NAFLD patients. The ROC curve showed that MHR or other inflammatory markers combined with traditional risk factors can improve the ability to predict CAD. In further study, trend chi-square showed that higher levels of MHR predicted more severe coronary lesions (P < 0.05). Multivariate ordinal logistic regression analysis showed that MHR was independently associated with changes in Gensini score (OR = 2.95, 95% CI 1.78–4.89, P < 0.001).
Conclusion
The MHR was positively associated with CAD risk among NAFLD patients and reflected coronary atherosclerosis severity.
Screening and identification of key chromatin regulator biomarkers for ankylosing spondylitis and drug prediction: evidence from bioinformatics analysis
Authors: Wanga Han , Jin Hongbo , Liu Zhiyang , Tan Chengju , Wei Lin , Fu Mingfen , Huang Yizhuan 
Published: November 2022

Abstract
Ankylosing spondylitis(AS) is one of the most common immune arthritic diseases in the world. Considerable efforts have been made to elucidate its pathogenesis, but the molecular mechanisms of ankylosing spondylitis are still not fully understood. To identify candidate genes in AS progression, the microarray dataset GSE25101 was downloaded from the Gene Expression Omnibus (GEO) database, differentially expressed genes (DEGs) were identified and functionally enriched for analysis, and a protein-protein interaction network (PPI) was constructed and performed using STRING and cytoHubba Modular analysis was performed, and immune cell and immune function analysis, functional analysis and drug prediction were performed. The results showed that DEGs were mainly associated with histone modifications, chromatin organization, transcriptional coregulator activity, transcriptional co-activator activity, histone acetyltransferase complexes, and protein acetyltransferase complexes. Differences in expression between the CONTROL and TREAT groups in terms of immunity were analyzed to determine the effect on TNF-α secretion. Two therapeutic agents, AY 11-7082 and Myricetin were predicted by obtaining hub genes. In conclusion, the DEGs, hub genes and predicted drugs identified in this study contribute to our understanding of the molecular mechanisms underlying the onset and progression of AS and provide candidate targets for the diagnosis and treatment of AS.
Unusual surface coagulation activation patterns of crystalline and amorphous silicate-based biominerals
Authors: Liu Chunyu , Cui Xu , Du Yunbo , Wang Xue , Kim Jua , Li Shuaijie , Zhang Liyan , Zhao Xiaoli , Zhao Limin , Tian Pengfei , Zhang Hao , Su Kun , Li Xian , Pan Haobo 
Published: November 2022

Abstract
Activation of coagulation cascades, especially FX and prothrombin, prevents blood loss and reduces mortality from hemorrhagic shock. Inorganic salts are effective but cannot stop bleeding completely in large hemorrhagic events, and rebleeding carries a significant mortality risk. The coagulation mechanism of inorganic salts has been oversimplified in the past two decades, limiting the creation of novel hemostats. Here, on the interface the activation of the coagulation, fibrinolysis, and cell activities were monitored at the protein level. The link between the hydrophilic-hydrophobic interface, hydration layer, microenvironmental structure of the crystal and amorphous salt, protease activity, and adsorption was also uncovered. It reveals that strong water binding and fibrinogen adsorption on kaolin's surface causes rebleeding after hemostasis, resulting in a weak thrombus. The kaolin surface inhibited the FIXa and FVIIIa composite assembly, reducing its positive feedback on the extrinsic pathway. Inspiringly, amorphous bioactive glass (BG) with transient-dynamic ions microenvironment interface are designed to bypass the barrier of the crystal structure hydration shell, hence enhancing the continuous activation of the biomaterial surface on coagulation system. Under comparative exploration, the unique coagulation pattern of BG was obtained: upon contact with the hydrophilic BGs, intrinsic and extrinsic coagulation pathways continuously initiated under the dynamic ionic microenvironment, and prothrombin complexes successfully hydrolyzed to thrombin without platelet membrane involvement, speeding the production of high-strength clots. Further evidence proves that BG more than doubled the survival rate of SD rats than kaolin in the lethal femoral artery, vein, and nerve disconnection hemorrhage model. This study investigates how the surface of inorganic salts assists in coagulation cascades that may help elucidate the clinical application of kaolin-gauze and pave the way to new materials for managing hemorrhage.
Impact of feed, light and access to fibres on tail biting in non-tail-docked pigs
Authors: Wallgren Per , Johansson Magnus , Wallgren Torun , Susic Zeljko , Sigfridson Kerstin , Johansson Sven-Erik 
Published: November 2022

Abstract
Background
Tail biting in pigs is a global welfare issue that has resulted in docking tails in many countries. However, tail docking by itself does not reduce the incitements for tail biting. Therefore, this study aimed to reduce tail biting in a high performing non-tail-docking pig herd with increasing incidences of tail biting.
Methods
The feed to all pigs was supplemented with amino acids, trace elements, vitamins and fibres. All pigs were also offered fibres in terms of hay silage. Thereafter four fattening stables were allotted into experimental groups as follows; I illuminated with standard fluorescent tubes with an invisible flickering of 30-40% for 14 hours per day; II illuminated with non-flickering led light for 14 hours per day; III illuminated with fluorescent tubes for 2 hours per day (this corresponded to the previous standard illumination and III served as a control group); IV similar illumination as III, but with increased access to hay silage.
Tail injuries were registered at slaughter during a period of four years prior to the trial, which was compared with the incidence of tail injuries during an adaptation period of six months and during the trial that lasted for a period of one year.
Results
The incidence of tail injuries during the four years that preceded the adaption period was 9.2%, and the incidence of tail injuries differed significantly between the buildings as well as with season.
During the adaption period, the incidence of tail injuries decreased significantly in all buildings and corresponded to 5.4%. The largest decrease was obtained in IV with extra access to hay silage where the incidence decreased from 11.4 to 4.3 %.
During the trial, the mean incidence of tail injuries continued to decrease to 3.0%. There were no significant differences between the stables.
Conclusions
The incidence of tail biting in fast growing non-tail-docked pigs was successfully reduced by supplementation of the feed with amino acids, trace elements, vitamins and fibres. Additional manipulative material in terms of hay silage accelerated that process and non-flickering illumination may have an impact in preventing tail biting.
The results obtained argue for the un-necessity of tail docking pigs, provided that the needs of the pigs in terms of feed ingredients, stocking density and access to manipulative materials are fulfilled.
Does Food Neophobia Affect Mediterranean Diet Adherence and Eating Disorders in University Students?
Authors: OZKAN Nilufer , GUL Fatma Hazan 
Published: November 2022

Abstract
Purpose
Food neophobia can describe the fear of the unfamiliar foods. It has many causes such as environmental, cultural, and genetic factors. This situation reduces the consumption of new or unfamiliar foods as well as other foods. The aim of this study is to investigate does food neophobia affect mediterranean diet adherence and eating disorders in university students.
Methods
In this cross-sectional study, 1277 university students were included between the ages of 18–24. The data were collected an online questionnaire. The questionnaire consisted of sociodemographic characteristics, Food Neophobia Scale (FNS), Mediterranean Type Diet Quality Index (KIDMED) and, Eating Disorder Examination Questionnaire (EDE-Q).
Results
Men's FNS scores (38.5 ∓ 10.97) were significantly lower than women (40.8 ∓ 9.21). Likewise, men's EDE-Q score (3.5 ∓ 3.32) was found to be significantly lower than females (4.4 ∓ 4.05). 69.5% of the individuals did not experience food neophobia, underweight (11.0%) were more neophobic than obese (4.4%) and there was no significant difference according to BMI classification (p > 0.05). A positive correlation was found between age and KIDMED, and a negative correlation was found between BMI and EDE-Q for the food neophobia.
Coclusion
There is a positive significant correlation between the EDE-Q score and subscales and BMI, and a negative significant relationship between KIDMED. A positive correlation was found between age and KIDMED, and a negative correlation was found between BMI and EDE-Q for the food neophobia. Food neophobia may affect mediterranean diet adherence and eating disorders in university students. Further research is recommended to obtain more certain data.
Level of evidence:
Level V, cross-sectional descriptive study.
The composition and natural variation of the skin microbiota in healthy Australian cattle
Authors: Gamage Hasinika K.A.H. , Vuong Daniel , Minns Scott A. , Chen Rachel , Piggott Andrew M. , Lacey Ernest , Paulsen Ian T. 
Published: November 2022

Abstract

Background Skin diseases in cattle pose significant animal welfare issues and financial burdens. Microbial communities inhabiting the skin have essential roles in maintaining host health. Microbiota manipulation can be an efficient strategy for improving the productivity and sustainability of skin disease management. However, a lack of information on the skin microbiota of healthy cattle and how environmental and host factors drive its normal variation has limited using skin microbes for diagnosing or treating skin disease and pest infestation in cattle. Here, we profiled the skin microbiota of 1,734 healthy cattle from 25 different herds located in a 187,000 km2area in the northeast to east Australia using 16S rRNA gene amplicon sequencing. The impact of host and climatic conditions on the skin microbial populations was examined. Results Our results demonstrate a highly diverse microbiota on the skin of healthy cattle. While the structure and diversity of microbial communities varied between herds, several bacteria were present consistently despite the differences in environmental conditions. For example, bacterial familiesMoraxellaceae,Sphingomonadaceae,BacillaceaeandBurkholderiaceaewere observed in most cattle, however, their relative abundance varied significantly between herds. Solar exposure and rainfall were key factors contributing to the observed variations in skin microbiota between herds, while temperature and cattle breed did not show any statistically significant impact on the composition of cattle skin microbiota. Conclusions This is the first report of the composition of the commensal skin microbiota of healthy cattle, specifically herds located in the northeast to east Australia and the impact of environmental and host variables on these microbial communities. Our study provides valuable insight into normal variation in cattle skin microbiota, an essential step for developing skin microbiota-based therapies for skin disease present in cattle.