bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence for translocation of oral Parvimonas micra from the subgingival sulcus of the human oral cavity to the colorectal adenocarcinoma
Authors: Conde-Pérez Kelly , Buetas Elena , Aja-Macaya Pablo , Arribas Elsa Martín-De , Iglesias-Corrás Iago , Trigo-Tasende Noelia , Nasser-Ali Mohammed , Estévez Lara S. , Rumbo-Feal Soraya , Otero-Alén Begoña , Noguera José F. , Concha Ángel , Pardiñas-López Simón , Carda-Diéguez Miguel , Gómez-Randulfe Igor , Martínez-Lago Nieves , Ladra Susana , Aparicio Luis M. A. , Bou Germán , Mira Álex , Vallejo Juan A. , Poza Margarita 
Published: October 2022

Abstract
Background: The carcinogenesis of colorectal cancer (CRC) is a multifactorial process involving both environmental and host factors, such as human genetics or the gut microbiome, which in CRC patients appears to be enriched in oral microorganisms. The aim of this work was to investigate the presence and activity of Parvimonas micrain CRC patients. To do that, samples collected from subgingival sulcus and neoplastic lesions were used for culturomics. Then, samples from different body locations (saliva, gingival crevicular fluid, feces, non-neoplastic colon mucosa, transition colon mucosa, adenocarcinoma, adenomas, metastatic and non-neoplastic liver samples) were used for 16S rRNA metabarcoding and metatranscriptomics. Whole genome sequencing was conducted for all P. micrastrains obtained.
Results: Several P. micraisolates from the oral cavity and adenocarcinoma tissue from CRC patients were obtained. The comparison of oral and tumoral P. micra genomes identified that a pair of clones (PM89KC) were 99.2% identical between locations in one CRC patient, suggesting that the same clone migrated from oral cavity to the gut. The 16S rRNA metabarcoding analysis of samples from this patient revealed that P. micra cohabits with other periodontal pathogens such as Fusobacterium, Prevotella or Dialister, both in the intestine, liver and the subgingival space, which suggests that bacterial translocation from the subgingival environment to the colon or liver could be more efficient if these microorganisms travel together forming a synergistic consortium. In this way, bacteria might be able to perform tasks that are impossible for single cells. In fact, RNA-seq of the adenocarcinoma tissue confirmed the activity of these bacteria in the neoplastic tissue samples and revealed that different oral species, including P. micra, were significantly more active in the tumor compared to non-neoplastic tissue from the same individuals.
Conclusion: P. micra appears to be able to translocate from the subgingival sulcus to the gut, where oral bacteria adapt to the new niche and could have a relevant role in carcinogenesis. According to our findings, periodontal disease, which increases the levels of these pathogens and facilitates their dissemination, could represent a risk factor for CRC development and P. micra could be used as a non-invasive CRC biomarker.
Increased distal femoral torsion is a predictor of non-contact anterior cruciate ligament injury: a case control study
Authors: Wang Dehua , Chen Jie , Fan Hengkai , Seeley Rebecca A. , Liang Xi , Huang Wei , Gan Xinling , Li Ke 
Published: October 2022

Abstract
Background
The Anterior cruciate ligament (ACL) is one of the most easily injured ligaments in the knee. Abnormal femur and knee rotation may lead to ACL injury, while an increased proportion of the lateral femoral condyle is also a potential factor in ACL injury. Therefore, distal femoral torsion (DFT) may be closely related to ACL injury. Thus, this study aims to investigate the associations of DFT with the risk of ACL injury and the severity of the injury.
Methods
582 patients who received ACL reconstruction surgery between 2014 and 2019 were examined retrospectively, and 114 patients were selected for analysis. Among them, 32 experienced ACL and anterolateral ligament (ALL) injury (ACL + ALL-injury group), and 32 experienced only ACL injury (only ACL injury group). In addition, 50 patients with similar age and BMI, but without ACL or ALL injury, were selected as the control group. DFT was assessed via the surgical posterior condylar angle (sPCA) on MRI by a blinded researcher. Inter-group differences were assessed via ANOVA. An ROC (Receiver Operating Characteristic) curve was generated to elucidate the ACL injury risk, and to establish the appropriate threshold value.
Result
The average sPCA was 3.68 ± 1.26° among the ACL + ALL-injury and ACL-injury only patients. Notably, the 3.77 ± 1.15° sPCA among the ACL + ALL-injury patients, and the 3.58 ± 1.37° sPCA among the ACL-injury patients were markedly enhanced, compared to the 2.41 ± 1.48° value in control patients. The ACL + ALL patients displayed an augmented sPCA trend over ACL-alone patients, however, it did not reach significance. Moreover, based on the ROC curve, sPCA > 2.37° was indicative of an enhanced ACL injury risk among the ACL-intact individuals.
Conclusion
Based on our analysis, augmented sPCA values were intricately linked to enhanced ACL injury risk in non-contact ACL ruptured patients. Nevertheless, its usage as a predictor of ACL injury severity is scarce. The conclusions of this study will likely assist orthopedic surgeons in identifying patients who may benefit from targeted ACL injury prevention.
Ability to track hemodynamic changes in parturients undergoing cesarean section using central venous pressure, stroke volume, and stroke volume variation: a prospective observational study
Authors: Li Chenchen , Zhang Xiaojun , Liu Chao , Li Liang 
Published: October 2022

Abstract
Fluid management in parturients with massive bleeding is often difficult to gauge because there are no reliable indicators of the fluid status. Thirty-two eligible parturients with pernicious placenta previa at the Qilu Hospital of Shandong University between January 2019 and August 2021 were included in this prospective observational study. All subjects were monitored continuously to measure the central venous pressure (CVP), stroke volume (SV), and stroke volume variation (SVV) along with standard monitoring. The primary outcome is the sensitivity and specificity of HR, CVP, SV, and SVV to diagnose hemodynamic instability induced by massive blood loss, which was evaluated by receiver operating characteristic (ROC) curve analysis. The areas under the ROC curve for HR, CVP, SVV, and SV to diagnose hemodynamic instability were 0.83 (sensitivity: 0.62, specificity: 1), 0.79 (sensitivity: 0.46, specificity: 1), 0.72 (sensitivity: 0.62, specificity: 0.95), and 0.64 (sensitivity: 0.69, specificity: 0.68), respectively. Massive blood loss, general anaesthesia induction, and balloon deflation caused significant changes in corresponding hemodynamic indicators. The CVP, SV, and SVV can effectively track the hemodynamic changes in parturients undergoing cesarean section. The sensitivity is low and the specificity is high for HR, CVP, and SVV to diagnose massive blood loss-induced hemodynamic instability.
Dissecting the tumorigenesis trajectory by single-cell RNA-seq reveals critical roles of RNA splicing
Authors: Wu Baohong , Chen Xuelan , Pan Xiangyu , Deng Xintong , Li Shujun , Wang Zhongwang , Wang Jian , Liao Dan , Xu Jing , Chen Mei , Zhao Chengjian , Xue Zhihong , Wang Yuan , Niu Ting , Lin Jingwen , Chen Lu , Chen Chong , Liu Yu 
Published: October 2022

Abstract
Background
Leukemogenesis is proposed to be a multistep process by which normal hematopoietic stem and progenitor cells are transformed into full-blown leukemic cells, the details of which are not fully understood.
Methods
We performed serial single-cell transcriptome analyses(10X genomics) of preleukemic and leukemic cells (PLCs) and constructed the cellular and molecular transformation trajectory in a Myc-driven AML model in mice, which represented the transformation course in patients. Another single-cell approach, Smart Seq2, was used to analyze alternative splicing events in leukemic cell.
Results
We found that the Myc targets were gradually upregulated along the trajectory. Among them were splicing factors, which showed stage-specific prognosis for AML patients. Furthermore, we dissected the detailed gene network of a tipping point for HSPCs to generate initiating PLCs, which was characterized by dramatically increased splicing factors and unusual RNA velocity. In the late stage, PLCs acquired explosive heterogeneity through RNA alternative splicing. Among them, the Hsp90aahi subpopulation was conserved in both human and mouse AML and associated with poor prognosis. Exon 6 skipping of Tmem134 was identified in these cells. While the exon skipping product Tmem134β promoted the cell cycle, full-length Tmem134α delayed tumorigenesis.
Conclusion
Our study emphasized the critical roles of RNA splicing in the full process of leukemogenesis and identified the role of a novel tumor specific alternative splicing event, Exon 6 skipping of Tmem134, during the occurrence and maintenance of leukemia.
NewRelics: an interactive installation development method for enhancing museum education
Authors: Qi Wenqian , Zhang Yebingqing , Zou Ning , Chen Chao , Sun Shouqian 
Published: October 2022

Abstract
This paper describes an innovative development method and how it supports museum education through providing better user experience of interactive installation. There have been many studies on museum interactive installations in recent years. We consider the weaknesses of existing installations, finding their interactions are static and lack tactile interaction simultaneously, without which visitors do not resonate with a cultural relic and will forget the experience quickly. To enhance further the user's experience of learning cultural knowledge when visiting the museum, we propose an interactive installation development method, NewRelics. We chose three characteristic cultural relics to demonstrate the whole process of NewRelics, and produce the installations. By operating them, visitors will make an impression on the cultural relics and their history, thus improving visitors' overall experience of the museum in the sense of visual, hearing, and touch, highlighting the significance of cultural relics. In the future, this method can be optimized in combination with smart devices using AR and VR to add value to person-object interaction, cultural experiences, and communication in a museum setting.
Contribution to the study of water resources in the coastal region of Essaouira, using vertical electrical soundings (VES), electrical resistivity tomography (ERT) and GIS (Essaouira, Morocco)
Authors: Ouzerbane Zakaria , Essahlaoui Ali , Hmaidi Abdellah El , Najine Abdessamad , Ouali Anas El 
Published: October 2022

Abstract
The geophysical study using the VES and the ERT, carried out in the coastal area of Essaouira (Morocco), for the purpose of mapping the aquifers by combining the two geo-electrical methods, the results allowed us to determine the lateral and vertical distribution of the geological formations in the study region. Thereafter the exploitation of these results by the decision makers in the choice of the sites likely to be hydrogeologically fertile to answer the need for the inhabitants in resource in water. The synthesis on the evolution of the geological layers, the variations of thickness and to highlight the anomalies which could affect them, the GIS tool makes it possible to draw up maps (VES) and electrical imagery (ERT ) at a depth chosen by data interpolated by Kriging. The analysis of the results shows that the Qsob zone is of paramount hydrogeological interest, it is represented by a very important layer given the great extension of the resistant formations of Plio-Plioctecene and Cretaceous age with significant thicknesses. The area is crossed by the Qsob River which is the main source of supply for these two aquifers. With the communication of the two aquifers by the very abundant and dense electric discontinuities of general directions E-W, NE-SW and NNE-SSW.
Evaluation and Spatial Distribution of Livelihood Capital at the District Level in the Koshi River Basin
Authors: Zhang Chenjia 
Published: October 2022

Abstract
Research of farmers’ livelihood capital contributes significantly to understanding the mechanism of regional poverty, advancing strategies to enhance the sustainability of poor farmers’ livelihoods, and formulating policies for regional targeted development. Taking the Nepalese and Chinese parts of the Koshi River Basin (KRB) as an example, this study investigates the current situation of shortages of livelihood capitals between the upper and lower reaches in China and Nepal separately, and explores the potential causes of differences in livelihood capital from the diverse perspectives. Results reveal that the comprehensive livelihood level in the basin is poor, and there are diverse levels of shortage and differentiation of livelihood capital. The total spatial difference in livelihood capital is minor and distributed at random or independently, and it was discovered that financial capital and social capital are the scarcest capital in the entire basin. These results also illustrate the spatial patterns of financial capital prevail in the Nepalese part of the basin. The livelihood capital at the district level is significantly related to adult literacy rates, levels of regional employment, the number of livestock at the end of the year, regional livestock product production, labor productivity, per capita of arable land area, crop production areas, and economically active populations. The findings produce a theoretical basis for the formulation of appropriate livelihood adaptation strategies and the establishment and enhancement of poverty reduction systems and policies in the KRB.
Regional Feature Learning using Structural Analysis of Attributes in a Bipartite Attention Framework for Vehicle Re-Identification
Authors: B Cynthia Sherin , Jayavel Kayalvizhi 
Published: October 2022

Abstract
Vehicle re-identification aspires to discover the target vehicle from the images obtained from the multiple non-overlapping real-world surveillance cameras. The challenges of illuminations, pose variations, resolution and various other attributes adds to the difficulty in re-identification. To overcome these difficulties, fine-grained visual differences in the vehicles are recognized for accurate predictions. Along with the coarse-grained features such as vehicle colour, model the customized features like annual service signs, logo, hangings are additionally considered. In this research work, we have designed a new dataset named Attributes27 with 27 classes of hierarchically labelled attributes to work on our proposed bipartite attention framework. The bipartite framework comprises of three blocks: A double branched convolution neural network layer extracts the global and local features in each vehicle image. Secondly, the self-attention block attached to each branch to detect the Region of Interests (ROIs). And finally the partition-alignment block is deployed to obtain the regional features from the gathered ROIs. Attributes27 and VeRi-776 datasets are the two datasets on which our proposed system is evaluated and the results show significant improvement in accuracy and also concentrate on the specific regional attributes present in each vehicle. The proposed method exhibits a performance of 98.5% and 84.3% respectively on Attributes27 and VeRi-776 datasets which is compared to be higher than the existing methods which exhibited 78.6% of accuracy.
Characterization and modeling of wood species sorption isotherms for use in civil engineering structures monitoring
Authors: Varnier Maximin , Ulmet Laurent , DUBOIS Frédéric , Sauvat Nicolas 
Published: October 2022

Abstract
This paper deals with a model allowing to calculate and describe, in a universal way, the sorption isotherms in wood material used in the moisture content measurement and the simulation of mass transfers. The originality of the presented tool lies in a thermodynamic approach explained in a few parameters model , grouped by species families, via the notion of normalized isotherms. It is also adapted to non-isothermal environments thanks to the application of Le Chatelier's principle. Finally, the model integrates partial isotherms, which makes it possible to use it not only to characterize the material sorption properties, but also to integrate it in heat transfer algorithms or in the implementation of moisture monitoring of timber structures under uncontrolled environments.
A multi-modal AI-driven cohort selection tool based on response to loading-phase aflibercept for neovascular age-related macular degeneration: PRECISE study
Authors: Chorev Michal , Haderlein Jonas , Chandra Shruti , Menon Geeta , Burton Benjamin , Pearce Ian , McKibbin Martin , Thottarath Sridevi , Karatsai Eleni , Chandak Swati , Kotagiri Ajay , Talks S , Grabowska Anna , Ghanchi Faruque , Gale Richard , Hamilton Robin , Antony Bhavna , Garnavi Rahil , Mareels Iven , Giani Andrea , Chong Victor , Sivaprasad Sobha 
Published: October 2022

Abstract

Patients diagnosed with neovascular age-related macular degeneration are commonly treated with anti-vascular endothelial growth factor (anti-VEGF) agents. However, response to treatment is heterogeneous, without a clinical explanation. Predicting suboptimal response at baseline will enable more efficient clinical trial designs for novel, future interventions and facilitate individualised therapies. In this multicentre study, we trained a multi-modal artificial intelligence (AI) system to identify suboptimal responders to the loading-phase of the anti-VEGF agent, aflibercept from baseline characteristics. We collected clinical features and optical coherence tomography scans from 1720 eyes of 1612 patients between 2019 and 2021. We evaluated our AI system as a patient selection method by emulating hypothetical clinical trials of different sizes based on our test set. Our method detected up to 57.6% more suboptimal responders than random selection, and up to 24.2% more than any alternative selection criteria tested. Applying this method to the entry process of candidates into randomised controlled trials may contribute to the success of such trials and further inform personalised care.
The Efficacy and Safety of Varenicline Nasal Spray for the Management of Dry Eye Signs: A Systematic Review and Meta-analysis
Authors: Bashrahil Bader , Taher Nada , Alzahrani Ziyad , Alnabihi Ahmed , Aldahlawi Abdulaziz , Alkhathlan Mohammad , Alghamdi Saeed 
Published: October 2022

Abstract

Background Dry eye disease (DED) is a disease caused by a reduced volume or deficient quality of tears and is treated mainly with tear supplementation. There is emerging evidence that nicotinic nasal sprays are effective for DED. Our systematic review and meta-analysis assessed the efficacy and safety of varenicline nasal spray (OC-01) for DED treatment utilizing the Grading of Recommendations Assessment, Development and Evaluation (GRADE) criteria. Methods Medline, Embase, and Cochrane Central Register of Controlled Trials (CENTRAL) databases were searched. Only randomized controlled trials (RCTs) that evaluated the efficacy of OC-01 versus placebo were included. The mean change in the Schirmer test score from baseline on day 28 was the efficacy endpoint. Serious adverse events (SAEs), nonserious adverse events (NSAEs), and the rate of mortality were the safety endpoints. The standardized mean difference (SMD) was used for continuous outcomes, while the risk ratio (RR) was used to demonstrate dichotomous variables. Data pooling was performed using the inverse variance weighting technique. The certainty of evidence was rated using the GRADE approach. The risk of bias assessment was conducted using the Revised Cochrane risk of bias tool for randomized trials. Results Three RCTs (n = 1063) met the eligibility criteria. All RCTs had a low risk of bias. Meta-analysis found a statistically significant increase in the mean STS change from baseline on day 28 (SMD = 5.70, 95% CI 3.40 − 7.99 P < 0.00001, I2 = 99%). The pooled analysis found no significant difference between OC-01 and placebo in the frequency of SAEs (RR = 0.99, 95% CI 0.49–2.00,P = 0.98, I2 = 8%) or mortality (RR = 1.05, 95% CI 0.16–7.16,P = 0.96, I2 = 0%). However, OC-01 had a significant effect on developing NSAEs (RR = 1.88, 95% CI 1.51–2.34,P < 0.00001, I2 = 75%). Conclusion OC-01 had a highly significant increase in the efficacy endpoints but caused an increased frequency of NSAEs. OC-01 did not correlate with either SAEs or mortality. The RCTs included in our study had a low risk of bias assessment. Nevertheless, they were downgraded in multiple domains upon GRADE assessment.
Venovenous extracorporeal membrane oxygenation for severe COVID-19 pneumonitis: case series from a Hungarian tertiary centre
Authors: Zöllei Éva , Rudas László , Hankovszky Péter , Korsós Anita , Pálfi Alexandra , Varga Zoltán , Tomozi László , Hegedüs Zoltán , Bari Gábor , Szivós Brigitta Lobozárné , Kiszel Attila , Babik Barna 
Published: October 2022

Abstract
Background: Venovenous extracorporeal membrane oxygenation (V-V ECMO) is recommended for the support of patients with severe COVID-19 pneumonitis. In this study we report the characteristics and outcome of COVID-19 patients treated with V-V ECMO in a Hungarian centre.

Methods: We retrospectively collected data on all patients admitted with proven SARS CoV-2 infection who received V-V ECMO support between March 2021 and May 2022.

Results: Eighteen patients were included in the analysis (5 women, age 44±10 years, APACHE II score 12 (IQR 10-14.5)). The patients had their first positive SARS-CoV-2 PCR test 9 (7-15) days before admission and were already hospitalised for 6 (4-11) days. Fifteen patients received non-invasive ventilation for 4 (2-8) days, two patients high flow nasal oxygen therapy each for one day. They were intubated 2.5 (1-6) days prior to ECMO initiation. Prone position was applied in 15 cases. The Lung Injury Score was 3.25 (3-3.26), the PaO2/FiO2 ratio was 71±19 mmHg. The duration of V-V ECMO support was 26±20 days, and the longest run lasted 70 days. Patients were mechanically ventilated for 34±23 days. Intensive care unit and hospital length of stay were 40±28 days and 45±31 days, respectively. 11 patients were successfully weaned from ECMO. ICU survival rate was 56%, in-hospital survival was 50%. All patients who were discharged from hospital reported a good health-related quality of life at the 5-16 month follow-up.

Conclusions: We achieved comparable survival rate to that reported by the European Chapter of the Extracorporeal Life Support Organisation COVID-19 Survey.