bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transapical beating heart mitral valve repair versus conventional surgery: a propensity-matched study
Authors: D’Onofrio A , Mastro F , Nadali M , Fiocco A , Pittarello D , Aruta P , Evangelista G , Lorenzoni G , Gregori D , Gerosa G 
Published: February 2022

Abstract

OBJECTIVES
Transapical Neochordae implantation (NC) allows beating heart mitral valve repair in patients with degenerative mitral regurgitation. The aim of this single-centre, retrospective study was to compare outcomes of NC versus conventional surgical (CS) mitral valve repair.

METHODS
Data of patients who underwent isolated mitral valve repair with NC or CS from January 2010 to December 2018 were collected. A propensity score matching analysis was performed to reduce confounding due to baseline differences between groups. The primary end point was overall all-cause mortality; secondary end points were freedom from reoperation, freedom from moderate (2+) and from severe (3+) mitral regurgitation (MR) and New York Heart Association functional class in the overall population and in patients with isolated P2 prolapse (type A anatomy).

RESULTS
Propensity analysis selected 88 matched pairs. There was no 30-day mortality in the 2 groups. Kaplan–Meier analysis showed similar 5-year survival in the 2 groups. Patients undergoing NC showed worse freedom from moderate MR (≥2+) (57.6% vs 84.6%; P &lt; 0.001) and from severe MR (3+) at 5-year follow-up: 78.1% vs 89.7% (P = 0.032). In patients with type A anatomy, freedom from moderate MR and from severe MR was similar between groups (moderate: 63.9% vs 74.6%; P = 0.21; severe: 79.3% vs 79%; P = 0.77 in NC and FS, respectively). Freedom from reoperation was lower in the NC group: 78.9% vs 92% (P = 0.022) but, in type A patients, it was similar: 79.7% and 85% (P = 0.75) in the NC and CS group, respectively. More than 90% of patients of both groups were in New York Heart Association class I and II at follow-up.

CONCLUSIONS
Transapical beating-heart mitral chordae implantation can be considered as an alternative treatment to CS, especially in patients with isolated P2 prolapse
Cervicotomy using a hemi-clamshell approach for a rare enlarged substernal goitre
Authors: Machboua Alia , Thumerel Mathieu , Hustache-Castaing Romain , Jougon Jacques 
Published: February 2022

Abstract
The substernal goitre is defined as a goitre for which &gt;50% of the mass is located below the superior orifice of the thorax, surgical resection remains the reference treatment, the approach used is the cervicotomy, which often allows to extract the mediastinal portion of the plunging goitre, and we report a rare case of a huge cancerous plunging goitre whose complete resection required the enlargement of the cervicotomy in right hemi-clamshell, for the carcinological, vascular and recurrent control.
Indigenous Lexicography: A Review of Recent Dictionaries and Works Relating to Lexicography
Authors: Turin Mark , Oliveira Ferreira Natália 
Published: February 2022

AbstractIn this review essay, we compare five recent publications relating to dictionary work with Indigenous languages. The review covers three dictionaries, one monograph about lexicography in service of Indigenous language revitalization and the second volume of a two-volume dictionary-cum-encyclopedia. The structure of this review essay is as follows: following a brief introduction to each of the languages covered in these five publications, we include sections comparing orthographic choices and representations, internal structure and entry design, an examination of each dictionary’s approach to the incorporation of neologisms and the software choices made by compilers. In addition, we offer an analysis of each dictionary’s intended audience and access requirements, some structured reflections on authorship and ownership, an exploration of each project’s commitment to community engagement, strategies for the representation of dialectal variation and finally, relevant information about how each dictionary project was funded and resourced.
Novel Spectrophotometric Approaches for the Simultaneous Quantification of Ternary Common Cold Mixture Containing Paracetamol with a Challenging Formulation Ratio: Greenness Profile Evaluation
Authors: Soliman Rabab M , Mostafa Nadia M , Fayez Yasmin M , Monir Hany H , Rostom Yasmin 
Published: February 2022

Abstract

Background
Common cold and cough preparations represent a huge segment of the global pharmaceutical market. Recently, cold/cough formulations containing paracetamol (PAR) have attracted significant attention as PAR has been implemented into the supportive treatment of mild cases of COVID-19 as the first-line antipyretic. From a literature review, no method has been reported yet for simultaneous estimation of PAR, pseudoephedrine hydrochloride (PSE) and carbinoxamine maleate (CRX) in any matrix. Thus, there is an urgent need for smart and green methods that would enable quantification of the cited components in their challenging ratio.

Objectives
The aim of this work is to develop and validate the first UV spectrophotometric methods for simultaneous determination of the selected drugs taking into consideration the list of challenges including the highly overlapping features and spectral interferences in the cited mixture.

Methods
Namely, the proposed methods are: direct spectrophotometry, dual wavelength, first derivative, derivative ratio, ratio difference, constant center coupled with spectrum subtraction, and the constant multiplication method paired with spectrum subtraction.

Results
These methods were linear over the concentration range of 2.5–35, 1.5–20, and 4.5–35 μg/mL for PAR, PSE and CRX, respectively. These methods fulfill the validity parameters according to International Conference on Harmonization (ICH) guidelines. The results obtained were statistically benchmarked to the official ones where no significant difference was noticed.

Conclusion
The developed methods are successfully applied for concurrent quantification of the studied components in the marketed dosage form without interference from matrix excipients. The impact on the environment was assessed by five green metrics, namely a recent Analytical greenness (AGREE) metric algorithm based on the green analytical chemistry framework, Green Analytical Procedure Index (GAPI), Eco-Scale, Assessment of Green Profile (AGP), and National Environmental Methods Index (NEMI).

Highlights
Eco-friendly and successive spectrophotometric methods were firstly developed in this work, for the simultaneous quantification of PAR, PSE and CRX. These approaches incorporate a simple enrichment-aided technique to augment their spectrophotometric signals, facilitating the accurate quantitation of the minor component in the cited mixture.
Debauchery and Original Sin: The Currency Composition of Sovereign Debt
Authors: Engel Charles , Park JungJae 
Published: February 2022

AbstractWe present a model that accounts for the “mystery of original sin” and the surge in local-currency borrowing by emerging economies in the recent decade. We quantitatively investigate the currency composition of sovereign debt in the presence of two types of limited enforcement frictions arising from a government's monetary and debt policy: strategic currency debasement and default on sovereign debt. Local-currency debt obligations act as a better consumption hedge against income shocks than foreign-currency debt because their real value can be affected by monetary policy. However, this provides a government with more temptation to deviate from disciplined monetary policy, thus restricting borrowing in local currency more than in foreign currency. Our model predicts that a country with a less credible monetary policy borrows mainly in foreign currency as a substitute for monetary credibility. An important extension demonstrates that in the presence of an expectational Phillips curve, local-currency debt improves the ability of monetary policymakers to commit.
Clonal integration affects growth and sediment properties of the first ramet generation, but not later ramet generations under severe light stress
Authors: Ma Xiaowen , Li Yang , Yu Weicheng , Wang Junnan , Liu Chunhua 
Published: February 2022

Abstract
Clonal integration benefits clonal plants by buffering environmental stress and increasing resource extraction efficiency. However, the number of connected ramet generations that benefit from clonal integration in a clonal system has received relatively little attention. A pot experiment was conducted to evaluate the extent of physiological integration within the clonal system of Vallisneria natans consisting of a mother ramet and three sequentially connected offspring ramets. Mother ramets were grown in full sunlight, and offspring ramets were heavily shaded with limited light availability. Stolons between mother ramets and offspring ramets were severed or connected, but connection among the three offspring ramets remained. The photosynthetic ability of unshaded mother ramets of V. natans was significantly enhanced, but their biomass accumulation was greatly reduced when connected to shaded offspring ramets. Clonal integration significantly increased biomass accumulation, C and N availabilities, extracellular enzyme activities and microbial biomass of the first ramet generation (adjacent ramet), but not later ramet generations. Our results indicate that support from the mother ramet of V. natans may be limited to the adjacent offspring ramet in a clonal system under severe light stress, implying an effect of ramet generation. Our results contribute to a better understanding of the hierarchy and segmentation of clonal plants. These findings suggest that the extent of clonal integration plays a vital role in ecological interactions of the ramet population.
Radix Ranunculi ternati: review of its chemical constituents, pharmacology, quality control and clinical applications
Authors: Wang Changlin , Wang Jieqiong , Gao Mingzhou , Gao Peng , Gao Dongmei , Zhang Hao , Mu Xiangyu , Qiao Mingqi 
Published: February 2022

Abstract

Objectives
Radix ranunculi ternati (RRT) is widely used as traditional and folk medicine distributed in China, as well as in Kyushu and Shikoku in Japan. Although RRT has a short history of use as medicine, it has a high medicinal value. This study summarizes the research progress on the chemical constituents, pharmacological activity, quality control measures and clinical applications to provide a valuable and exhaustive reference for the development and application of RRT.

Key findings
Phytochemical studies showed that this plant mainly contains glycosides, organic acids, esters, sterols, flavonoids, alkaloids and volatile oils. The pharmacological activity of RRT includes immune regulation, anti-tumour, anti-tuberculosis, antioxidant, antibacterial, hepatoprotective and anti-inflammatory effects. This plant is especially effective in the treatment of cancer, tuberculosis, thyroid and nasopharynges disorders.
Tick-borne encephalitis vaccine—a wave of news
Authors: Steffen Robert , Schmitt Heinz-Josef , Zavadska Dace 
Published: February 2022

Tick-borne encephalitis vaccine efficacy approximates 90% after the initial two doses, but the duration of protection is unknown. Emerging evidence indicates that vaccine effectiveness does not depend on the seropersistence of antibodies but on the boostability. Thus, conventional recommendations with booster doses every 3 or 5 years need to be reconsidered.
Implementing the Reichsbürgergesetz: Registration, Statistics, and the Deportations of German Jews
Authors: Boberg Stefan 
Published: February 2022

Abstract
This article reconsiders the process by which Jews were excluded from the National Socialist state in the 1940s by analysing its administrative preconditions. It focuses on the identification of those individuals in the ‘Altreich’—pre-war German territory—who were defined as Jewish or of ‘mixed blood’ by the 1935 Reichsbürgergesetz (Reich Citizenship Law). Due to the prior absence of criteria, the number and identities of this group were unknown to the authorities. After summarizing historiographical discussions about the involvement of statisticians in the identification and registration of German Jews, this article argues that the hitherto neglected Volkskartei (People’s Card Index) was as important for the localization of Germany’s Jews prior to the deportations as the 1939 census was for their identification. Only the coordinated interaction of both devices made it possible to aggregate and provide the required information to facilitate National Socialist deportations.
Pilot-WINGS: An extended MUSE view of the structure of Abell 370
Authors: Lagattuta David J , Richard Johan , Bauer Franz Erik , Cerny Catherine , Claeyssens Adélaïde , Guaita Lucia , Jauzac Mathilde , Jeanneau Alexandre , Koekemoer Anton M , Mahler Guillaume , Prieto Lyon Gonzalo , Acebron Ana , Meneghetti Massimo , Niemiec Anna , Zitrin Adi , Bianconi Matteo , Connor Thomas , Cen Renyue , Edge Alastair , Faisst Andreas L , Limousin Marceau , Massey Richard , Sereno Mauro , Sharon Keren , Weaver John R 
Published: February 2022

ABSTRACT
We investigate the strong-lensing cluster Abell 370 (A370) using a wide Integral Field Unit (IFU) spectroscopic mosaic from the Multi-Unit Spectroscopic Explorer (MUSE). IFU spectroscopy provides significant insight into the structure and mass content of galaxy clusters, yet IFU-based cluster studies focus almost exclusively on the central Einstein-radius region. Covering over 14 arcmin2, the new MUSE mosaic extends significantly beyond the A370 Einstein radius, providing, for the first time, a detailed look at the cluster outskirts. Combining these data with wide-field, multi-band Hubble Space Telescope (HST) imaging from the BUFFALO project, we analyse the distribution of objects within the cluster and along the line of sight. Identifying 416 cluster galaxies, we use kinematics to trace the radial mass profile of the halo, providing a mass estimate independent from the lens model. We also measure radially averaged properties of the cluster members, tracking their evolution as a function of infall. Thanks to the high spatial resolution of our data, we identify six cluster members acting as galaxy–galaxy lenses, which constrain localized mass distributions beyond the Einstein radius. Finally, taking advantage of MUSE’s 3D capabilities, we detect and analyse multiple spatially extended overdensities outside of the cluster that influence lensing-derived halo mass estimates. We stress that much of this work is only possible thanks to the robust, extended IFU coverage, highlighting its importance even in less optically dense cluster regions. Overall, this work showcases the power of combining HST + MUSE, and serves as the initial step towards a larger and wider program targeting several clusters.
The dynamics and outcome of star formation with jets, radiation, winds, and supernovae in concert
Authors: Grudić Michael Y , Guszejnov Dávid , Offner Stella S R , Rosen Anna L , Raju Aman N , Faucher-Giguère Claude-André , Hopkins Philip F 
Published: February 2022

ABSTRACTWe analyse the first giant molecular cloud (GMC) simulation to follow the formation of individual stars and their feedback from jets, radiation, winds, and supernovae, using the STARFORGE framework in the GIZMO code. We evolve the GMC for $\sim 9 \rm Myr$, from initial turbulent collapse to dispersal by feedback. Protostellar jets dominate feedback momentum initially, but radiation and winds cause cloud disruption at $\sim 8{{\ \rm per\ cent}}$ star formation efficiency (SFE), and the first supernova at $8.3\, \rm Myr$ comes too late to influence star formation significantly. The per-free-fall SFE is dynamic, accelerating from 0 per cent to $\sim 18{{\ \rm per\ cent}}$ before dropping quickly to &lt;1 per cent, but the estimate from YSO counts compresses it to a narrower range. The primary cluster forms hierarchically and condenses to a brief ($\sim 1\, \mathrm{Myr}$) compact ($\sim 1\, \rm pc$) phase, but does not virialize before the cloud disperses, and the stars end as an unbound expanding association. The initial mass function resembles the Chabrier (2005) form with a high-mass slope α = −2 and a maximum mass of 55 M⊙. Stellar accretion takes $\sim 400\, \rm kyr$ on average, but $\gtrsim 1\,\rm Myr$ for &gt;10 M⊙ stars, so massive stars finish growing latest. The fraction of stars in multiples increase as a function of primary mass, as observed. Overall, the simulation much more closely resembles reality, compared to previous versions that neglected different feedback physics entirely. But more detailed comparison with synthetic observations will be needed to constrain the theoretical uncertainties.
GroEL/S Overexpression Helps to Purge Deleterious Mutations and Reduce Genetic Diversity during Adaptive Protein Evolution
Authors: Iyengar Bharat Ravi , Wagner Andreas 
Published: February 2022

Abstract
Chaperones are proteins that help other proteins fold. They also affect the adaptive evolution of their client proteins by buffering the effect of deleterious mutations and increasing the genetic diversity of evolving proteins. We study how the bacterial chaperone GroE (GroEL+GroES) affects the evolution of green fluorescent protein (GFP). To this end, we subjected GFP to multiple rounds of mutation and selection for its color phenotype in four replicate Escherichia coli populations, and studied its evolutionary dynamics through high-throughput sequencing and mutant engineering. We evolved GFP both under stabilizing selection for its ancestral (green) phenotype, and to directional selection for a new (cyan) phenotype. We did so both under low and high expression of the chaperone GroE. In contrast to previous work, we observe that GroE does not just buffer but also helps purge deleterious (fluorescence reducing) mutations from evolving populations. In doing so, GroE helps reduce the genetic diversity of evolving populations. In addition, it causes phenotypic heterogeneity in mutants with the same genotype, helping to enhance their fluorescence in some cells, and reducing it in others. Our observations show that chaperones can affect adaptive evolution in more than one way.
i-Motif formation and spontaneous deletions in human cells
Authors: Martella Marianna , Pichiorri Flavia , Chikhale Rupesh V , Abdelhamid Mahmoud A S , Waller Zoë A E , Smith Steven S 
Published: February 2022

Abstract
Concatemers of d(TCCC) that were first detected through their association with deletions at the RACK7 locus, are widespread throughout the human genome. Circular dichroism spectra show that d(GGGA)n sequences form G-quadruplexes when n &gt; 3, while i-motif structures form at d(TCCC)n sequences at neutral pH when n ≥ 7 in vitro. In the PC3 cell line, deletions are observed only when the d(TCCC)n variant is long enough to form significant levels of unresolved i-motif structure at neutral pH. The presence of an unresolved i-motif at a representative d(TCCC)n element at RACK7 was suggested by experiments showing that that the region containing the d(TCCC)9 element was susceptible to bisulfite attack in native DNA and that d(TCCC)9 oligo formed an i-motif structure at neutral pH. This in turn suggested that that the i-motif present at this site in native DNA must be susceptible to bisulfite mediated deamination even though it is a closed structure. Bisulfite deamination of the i-motif structure in the model oligodeoxynucleotide was confirmed using mass spectrometry analysis. We conclude that while G-quadruplex formation may contribute to spontaneous mutation at these sites, deletions actually require the potential for i-motif to form and remain unresolved at neutral pH.
COP1 positively regulates ABA signaling during Arabidopsis seedling growth in darkness by mediating ABA-induced ABI5 accumulation
Authors: Peng Jing , Wang Meijiao , Wang Xiaoji , Qi Lijuan , Guo Can , Li Hong , Li Cong , Yan Yan , Zhou Yun , Terzaghi William , Li Zhen , Song Chun-Peng , Qin Feng , Gong Zhizhong , Li Jigang 
Published: February 2022

AbstractCONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1), a well-characterized E3 ubiquitin ligase, is a central repressor of seedling photomorphogenic development in darkness. However, whether COP1 is involved in modulating abscisic acid (ABA) signaling in darkness remains largely obscure. Here, we report that COP1 is a positive regulator of ABA signaling during Arabidopsis seedling growth in the dark. COP1 mediates ABA-induced accumulation of ABI5, a transcription factor playing a key role in ABA signaling, through transcriptional and post-translational regulatory mechanisms. We further show that COP1 physically interacts with ABA-hypersensitive DCAF1 (ABD1), a substrate receptor of the CUL4-DDB1 E3 ligase targeting ABI5 for degradation. Accordingly, COP1 directly ubiquitinates ABD1 in vitro, and negatively regulates ABD1 protein abundance in vivo in the dark but not in the light. Therefore, COP1 promotes ABI5 protein stability post-translationally in darkness by destabilizing ABD1 in response to ABA. Interestingly, we reveal that ABA induces the nuclear accumulation of COP1 in darkness, thus enhancing its activity in propagating the ABA signal. Together, our study uncovers that COP1 modulates ABA signaling during seedling growth in darkness by mediating ABA-induced ABI5 accumulation, demonstrating that plants adjust their ABA signaling mechanisms according to their light environment.
The evolutionary history of small RNAs in Solanaceae
Authors: Baldrich Patricia , Bélanger Sébastien , Kong Shuyao , Pokhrel Suresh , Tamim Saleh , Teng Chong , Schiebout Courtney , Gurazada Sai Guna Ranjan , Gupta Pallavi , Patel Parth , Razifard Hamid , Nakano Mayumi , Dusia Ayush , Meyers Blake C , Frank Margaret H 
Published: February 2022

AbstractThe Solanaceae or “nightshade” family is an economically important group with remarkable diversity. To gain a better understanding of how the unique biology of the Solanaceae relates to the family’s small RNA (sRNA) genomic landscape, we downloaded over 255 publicly available sRNA data sets that comprise over 2.6 billion reads of sequence data. We applied a suite of computational tools to predict and annotate two major sRNA classes: (1) microRNAs (miRNAs), typically 20- to 22-nucleotide (nt) RNAs generated from a hairpin precursor and functioning in gene silencing and (2) short interfering RNAs (siRNAs), including 24-nt heterochromatic siRNAs typically functioning to repress repetitive regions of the genome via RNA-directed DNA methylation, as well as secondary phased siRNAs and trans-acting siRNAs generated via miRNA-directed cleavage of a polymerase II-derived RNA precursor. Our analyses described thousands of sRNA loci, including poorly understood clusters of 22-nt siRNAs that accumulate during viral infection. The birth, death, expansion, and contraction of these sRNA loci are dynamic evolutionary processes that characterize the Solanaceae family. These analyses indicate that individuals within the same genus share similar sRNA landscapes, whereas comparisons between distinct genera within the Solanaceae reveal relatively few commonalities.