bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacillus sp. DU-106 ameliorates anti-diabetic effect by modulating gut microbiota in high-fat-fed and streptozotocin-induced type 2 diabetic mice
Authors: Yan Jing , Li Junjian , Xue Qiuyan , Xie Shiqing , Jiang Jinjin , Li Pan , Du Bing 
Published: April 2022

Abstract
Type 2 diabetes (T2D) is a chronic disease that manifests with endocrine and metabolic disorders, seriously threatening public health. This study aims to investigate the effects of Bacillus sp. DU-106 on anti-diabetic and gut microbiota in C57BL/6J mice on a high-fat diet (HFD) and streptozotocin (STZ)-induced T2D. Bacillus sp. DU-106 was administered to model mice for 8 consecutive weeks. Oral administration of Bacillus sp. DU-106 led to decreased food and water intake and alleviated body weight loss. And Bacillus sp. DU-106 imparted several health benefits to the mice, including balanced blood glucose, alleviation of insulin resistance in the T2D mice, and an improvement in lipid metabolism. Furthermore, Bacillus sp. DU-106 protected against liver and pancreas impairment to some extent. Additionally, Bacillus sp. DU-106 treatment reshaped the intestinal flora by enhancing gut microbial diversity and enriching the abundance of certain functional bacteria. Collectively, these findings suggested that Bacillus sp. DU-106 has the potential to ameliorate T2D by regulation of the gut microbiota. Therefore, a novel probiotic proposed here, Bacillus sp. DU-106 might be a promising therapeutic agent to prevent and alleviate T2D in mice.
Description and interpretation of the lived experience of hematopoietic stem cell transplantation, focusing on before, during, and after transplantation: A hermeneutic phenomenological approach
Authors: Rambod Masoume , Pasyar Nilofar , Zarshenas Ladan , Bagheri Shahpar , Ramzi Mani 
Published: April 2022

Abstract
Purpose: This study aimed at description and interpretation of the lived experience of Hematopoietic Stem Cell Transplantation (HSCT) as a novel therapeutic option in hematologic cancers.Methods: This hermeneutic phenomenological study was conducted on 13 individuals who had undergone HSCT. The participants were selected using purposeful sampling. In-depth interview was used and data analysis was conducted using van Manen’s methodological framework.Results: Three themes emerged in this study, including “bewilderment in the shadow of hope”, “emerging dream of budding”, and “entering the calm beach”. Bewilderment in the shadow of hope consisted of four subthemes, namely “strange and shocking news”, “searching for information accomplished with psychological distress”, “having some misconceptions”, and “finding a chance for survival”. In addition, five subthemes of “chemotherapy as a root of transplantation”, “immersing in the psychological burden”, “shining light of life”, “waiting for successful transplantation”, and “living with positive feelings” emerged from “emerging dream of budding”. Finally, “entering the calm beach” consisted of “resilience in spite of physical burden”, “constant attention to blood cells count”, “conducting careful self-care”, “having family support”, “strong relationship with God”, and “valuable rebirth and continuation of life”.Conclusion: Being informed about the need for HSCT was shocking and led to psychological distress and misconceptions before HSCT performance. However, entrance of stem cells into the body shined the light of life and the participants spent their hospitalization period with positive feelings. After HSCT, psychosocial and spiritual interventions are suggested to be provided to improve calm life among individuals.
Association of PRLR, IGF1 and LEP genes polymorphism with milk production and litter size in Egyptian Zaraibi goat
Authors: El-Shorbagy Haidan Mostafa , Abdel-Aal Ehab Salah , Mohamed Shaimaa Abdel-Azeem , El-Ghor Akmal Abdel-Reheim 
Published: April 2022

Abstract
This study aimed to detect specific nucleotide polymorphisms in Prolactin receptor (PRLR), Insulin like growth factor (IGF1) and Leptin (LEP) genes and their correlation with milk production (MP) and litter size (LS) traits in Zaraibi goat. PCR-SSCP products of different patterns of each gene were sequenced and aligned to reveal two single nucleotide polymorphisms (SNPs) (T>C) and (G>A) in 3’UTR of PRLR gene and registered with accession numbers OM418863 for TT and OM418864 for CT, while (G>A) SNP was registered as OM418861 for GG and OM418862 for AG in exon 10. TT, CT, AG and GG genotypes were distributed in the studied animals with frequencies 0.43, 0.57, 0.65 and 0.35, respectively. While alleles C, T, A and G frequencies were 0.28, 0.72, 0.32 and 0.68, respectively. CT and AG genotypes associated significantly (P<0.05) with higher MP and LS respectively. By studying the haplotypes of PRLR, C-A and T-A were associated with the highest and the lowest level of MP respectively. For LS, T-A and C-G showed significant correlation with the highest and the lowest rate respectively. Regarding IGF1 gene, two SNPs were detected; T74C at exon 4 which registered as (OM418860), and combined SNPs as ins. G470, A531G and T534C (PP genotype) at 5’ flanking region that registered as (OM418859). For LEP, only one SNP was found in intron 2 (G281A) which submitted to GenBank as (OM418855). All detected SNPs have shown to be involved in regulating the MP or LS as reproductive traits in goat.
The Impact of China's Environmental Regulation on Total Factor Productivity of Pharmaceutical Manufacturing Industry: An Analysis Based on the Spatial Spillover Effect
Authors: Wu Qijie , Li Yuexin , Wu Yufei , Li Fei , Zhong Shen 
Published: April 2022

Abstract
As an important embodiment of a country's economic strength and national health, pharmaceutical manufacturing industry has made rapid development in China in recent years. But at the same time, the pharmaceutical manufacturing industry is facing many environmental problems, such as large pollution emissions, complex pollution components, controlling difficulties and so on. This paper measures the total factor productivity of pharmaceutical manufacturing industry (HTFP) by using data envelopment analysis (DEA), and studies the effect of environmental regulation (ERI) on the total factor productivity of pharmaceutical manufacturing industry (HTFP) by establishing panel data regression model and spatial econometric model based on 30 provinces in China from 2004 to 2019. The conclusions are as follows: (1) Environmental regulation and total factor productivity of pharmaceutical manufacturing industry have significant spatial autocorrelation, showing "high-high" or "low-low" spatial aggregation characteristics; (2) Environmental regulation has a significant promoting effect on improving HTFP in local and surrounding areas, and there are differences in the impact of eastern, central and western regions; (3) Green technology, production technology and industrial structure play an important role in the impact of ERI on HTFP, which provides theoretical guidance and policy recommendations for improving the level of total factor productivity of pharmaceutical manufacturing industry in the environmental aspect.
A kinematic precision reliability evaluation method for rotor-bearing systems considering multi-source wear degradations and random errors
Authors: Wang Hongwei , Xiang Jiawei , Zhao Xufeng , Li Yulong 
Published: April 2022

Abstract
Rotor-bearing systems play a vital role in machine tools, aero engines, and wind turbines. Generally, worn-induced degradation quantities and manufacturing errors of components are the main error sources that influence the precision reliability of rotor-bearing systems. The current precision reliability evaluation models are focusing on several error sources in only a few key components without agreeable results. Therefore, a precision reliability evaluation model is proposed considering all time-variant error sources and random error sources. Firstly, time-variant wear models for commonly occurred degradation types in a rotor-bearing system are developed. Secondly, the constructed time-variant wear models are inserted into the precision model with all moving components in the rotor-bearing system using the Meta-Action structural decomposition method. Finally, the time-variant stochastic process discretization method is employed to establish the precision reliability evaluation model, and solve the precision reliability of the rotor-bearing systems. Case investigations are carried out to verify the performance of the present model, which provides a better precision reliability evaluation model to estimate the conditions of rotor-bearing systems during the service period.
Unraveling rationale behind tomato (Solanum Lycopersicum L.) shelf life: Role of fruit biochemical, morpho- physiological traits in extending shelf life
Authors: M.P. Pavan , S. Gangaprasad , Kumar Niranjana , Adivappar Nagarajappa 
Published: April 2022

Abstract
Prolonging the keeping quality of tomatoes is very essential for reduction of great losses in quality and quantity. Fruit shelf life is ripening-associated complex trait affected by many quantitative fruit quality traits. In this study, 30 tomato germplasm lines were characterized for very important less pursued shelf life and its contributing traits. Shelf life ranged from 10.50 to 34.43 days. Slower dehydration rate noticed in tender fruit tissues in turning stage. Slow pace in rate of change in physiological losses in fruit weight led to high shelf life. Fruit firmness ranged from 2.49 to 5.76 kg/cm2 and pericarp thickness from 1.33 to 6.70 mm. High shelf life germplasm lines contained 6.98–19.29 mg/100g and 8.64–13.38 mg/100g of fruit calcium and magnesium content respectively. The fruit epicuticular wax content varied from 34.64 to 96.77 µg/cm2. Calcium, magnesium, and epicuticular wax decrease respiration rate, maintain firmness, delay senescence, reduces incidence of physiological disorders and extend fruit storage life. Higher shelf life in germplasm lines P-4, L-00191, L-03686, AR-23, PKM-1, and Bony best are contributed by higher firmness and epicuticular wax content and high shelf life in Red ball is due to higher firmness, pericarp thickness, calcium, magnesium and epicuticular wax content.
Feeding behaviour of Foot and Mouth Disease (FMD) infected Holstein Friesian crossbred calves offered with therapeutic diet
Authors: Somagond Arun , Patel B. H. Manjunatha , Pattanaik A K , Verma M. R. , Dutt Triveni , Bhanuprakash V. 
Published: April 2022

Abstract
Foot and Mouth Disease (FMD) affects dry matter intake (DMI) and performance due to mucosal erosions developed on the tongue and gums. Therapeutic diets (TD) in a physical form compatible with the inflammatory condition of the mouth ensures optimum intake with changed behavioural pattern. The present study aimed to record the feeding behaviour of FMD infected calves by offering a therapeutic diet. A total of 22 Holstein Friesian crossbred male calves (10–12 months) were used in which 4 calves were of Control (CON) and 18 calves of treatment groups viz. Therapeutic Diet- Mash (TDM), Therapeutic Diet-Cooked (TDC), Therapeutic Diet-Customized Nutrient Supplement (TDCNS) were infected with FMD virus in the bio-containment facility (BSL-III). The therapeutic diet (TD) was fed to calves in the forenoon session and ad libitum hybrid Napier in afternoon session. The diet had high protein and energy with varied physical forms (mash or cooked). The infected group calves experienced difficulty in eating viz. 0.00 ± 0.00, 19.57 ± 3.19, 25.48 ± 4.13 & 27.30 ± 4.38 min in CON, TDM, TDC, TDCNS respectively. The time values were highly significant between the groups (p < 0.01). Licking behaviour was noticed only in the morning session. Feed tossing, ruminating behaviour was noticed in the afternoon session in all the animals irrespective of groups. The frequency of urination was more in the morning and the defecation was comparable between the groups. The lethargy score was high for 3 days post-infection in the infected groups and subsequently declined. Non-nutritive behaviour is not seen either in the forenoon or in the afternoon session. In conclusion, tailor made therapeutic diets with changed physical forms (mash or cooked) was compatible with the inflammatory condition of the mouth.
Implementation and Evaluation of a Discharge Planning for Patients Undergoing Umbilical Cord Blood Transplantation
Authors: Huang Lu , Zhu Yan , Wu Yun , Wang Ying-Ying , Song Gui-qi , Song Kai-di , Wu Yao-hua , Zhang Yong-Liang 
Published: April 2022

Abstract

Purpose
The purpose of this study was to implement a discharge planning for patients undergoing umbilical cord blood transplantation (UCBT) and evaluate the effects on discharge readiness, length of stay, hospital readmission, self-efficacy, and quality of life (QoL).
Methods
Participants (n = 66) were assigned to a control group (CG: received usual care) or an intervention group (IG: received discharge planning). Discharge readiness, length of stay, and the rate of hospital readmission were collected. Additionally, self-efficacy and quality of life survey (FACT-BMT) were assessed at hospital admission (T1), discharge (T2), and approximately Day 100 after UCBT (T3).
Results
The discharge planning group showed significantly better discharge readiness (p < 0.001), improvement in self-efficacy (increased by 11.04% at T3), the time effect (p = 0.035) and interaction effect (p = 0.027) were significant. Except for emotional and social well-being, all the other dimensions and three total scores of FACT-BMT in the IG were higher than controls, the main effect of group was significant (p < 0.05). However, discharge planning did not reduce patients’ length of stay or hospital readmissions.
Conclusion
This study provided the evidences on the implementation of discharge planning for patients undergoing UCBT, and suggested that nurse-led discharge planning should be incorporated into usual care for successful hospital-to-home transitions.
Subacute and chronic proteomic and phosphoproteomic analyses of a mouse model of traumatic brain injury at two timepoints and the comparison with chronic traumatic encephalopathy in human samples.
Authors: Morin Alexander , Roderick Davis , Teresa Darcey , Benoit Mouzon , Crawford Fiona 
Published: April 2022

Abstract
Repetitive mild traumatic brain injury (r-mTBI) is the most widespread type of brain traumas worldwide. The cumulative injury effect triggers long-lasting pathological and molecular changes that may increase a risk of chronic neurodegenerative diseases. R-mTBI is also characterized by changes in brain proteome, where the majority of molecules altered early post-TBI are different from those altered at more chronic phases. This differentiation may contribute to heterogeneity of available data on potential therapeutic targets and may present an obstacle in developing effective treatments. Here, we aimed to characterize a proteome profile of r-mTBI in a mouse model at two time points – 3 and 24 weeks post last TBI, as this may be a more relevant therapeutic window for individuals suffering negative consequences of r-mTBI. We identified a great number of proteins and phosphoproteins that remain continuously dysregulated from 3 to 24 weeks. These proteins may serve as effective therapeutic targets for sub-acute and chronic stages of post r-mTBI. We also compared canonical pathways activation associated with either total proteins or phosphoproteins and revealed that they both are upregulated at 24 weeks. However, at 3 weeks post-TBI, only pathways associated with total proteins are upregulated, while pathways driven by phosphoproteins are downregulated. Finally, to assess the translatability of our data, we compared proteomic changes in our mouse model with those reported in human samples of Chronic Traumatic Encephalopathy (CTE) compared to controls. We observed 39 common proteins that were upregulated in both species and 24 common pathways associated with these proteins. These findings support the translational relevance of our mouse model of r-mTBI for successful identification and translation of therapeutic targets.
Hepatitis B core antibody (HBcAb)-positive is significantly correlated with hilar cholangiocarcinoma and affects operative safety of major hepatectomy in Chinese patients
Authors: Huang Zhiyong , Wang Wenqiang , Xu Guangyuan , Zhang Erlei , Liang Binyong , Li Jiang , Li Jian , Lin Meilong , Chen Xiaoping 
Published: April 2022

Abstract
Background: Hepatitis B virus (HBV) may be a risk factor for cholangiocarcinoma, but it is not yet clear that the relationship between hepatitis B core antibody (HBcAb)-positive and hilar cholangiocarcinoma (hCCA). In addition, we evaluated the impact of HBcAb-positive on the safety of major hepatectomy for hCCA. Methods：A retrospective analysis was performed on the status of hepatitis B infection, liver fibrosis, surgical safety, and long-term postoperative survival of 111 patients who underwent major hepatectomy in Tongji Hospital from April 2012 to September 2019.Results: HBsAg-positive was identified in 10.6%, HBcAb-positive was found in 73.7% of the patients. A high-grade of liver fibrosis was detected in 67.9% of the patients with HBcAb-positive, which was markedly higher than that in the patients with HBcAb-negative (43.3%) (P=0.018). The incidence rates of postoperative complications and 30-day mortality were 38.7% (43/111) and 5.4% (6/111), respectively. Multivariate analysis showed that the independent risk factors for complications were HBcAb, vascular reconstruction, lymphadenectomy, HBcAb-positive correlated with a higher incidence of complications after major hepatectomy for hCCA (adjusted hazard ratio [HR] 3.984, 95%CI 1.354-11.73; P = 0.012). All the patients who died within 30-day after surgery were HBcAb-positive. However, there was no correlation between HBcAb and long-term prognosis of major hepatectomy for hCCA.Conclusions: HBcAb-positive is closely correlated with hCCA. HBcAb-positive markedly increased the incidence of postoperative complications and mortality of major hepatectomy for hCCA.
Clinicopathological Characterization of Small Cell Synovial Sarcoma: A Case Report
Authors: Qin Lingyan , Yang Hongli , Guo Lingchuan , Ma Wenxia , Gu Dongmei 
Published: April 2022

Abstract
Background
As an umbrella term of malignant tumor, synovial sarcoma (SS) is, characterized by bidirectional differentiation and harboring of SYT-SSX1 fusions, with a rare subtype, small cell synovial sarcoma (SCSS), highly malignant. In this paper, we reported a case of SCSS and reviewed related literature.
Case Presentation:
A 39-year-old women visited our hospital due to chest tightness for over two years along with serious cough. The outpatient chest CT scan revealed a mass in the upper right lung. Further examinations such as contrast-enhanced CT and Fiberoptic bronchoscopy at our hospital indicated a malignant lung tumor. Thereby the ultrasound-guided needle biopsy was performed on the patient for further diagnosis. Postoperative histological examination suggested that the tumor cells were oval or short fusiform with variable size. The tumor cells presented the form of nests, with less cytoplasm and weak eosinophilia. The nucleus was round or oval with densely stained chromatin, while naked nuclei identifiable in the background. All these findings indicated inclination to SCSS, which required further confirmed diagnosis by the results of immunohistochemical analysis and FISH tests. The patient underwent periodic chemotherapy after the confirmation of diagnosis, with no recurrence within 36 months of follow-up, which unfortunately, was available as of the 42nd month.
Conclusions
SCSS is a scarce tumor without prominent specificity in terms of clinical manifestations. Diagnosis and differential diagnosis require a combination of pathological characterization, immunophenotyping and molecular genetic testing. The clinical regimens mainly rely on the complete surgical resection of the mass whereas chemotherapy and radiotherapy reportedly improve the prognosis of patients.
Behaviors of Chemical Anchors Installed in Different Types of Concrete
Authors: ÇALIŞKAN Özlem , KARAKURT Cenk , ARAS Murat , KAYA Turgut 
Published: April 2022

Abstract
Chemical anchors are widely used in additional installations of construction irons, repair and reinforcement work. Due to the increasing diversity of materials and the improved awareness of waste products, it has become possible to produce different types of concrete with various characteristics. In this study, class B420C ribbed bars with 16 mm diameter were installed in four different concrete types by using four different chemical adhesives, and a tensile force was applied. The stiffness, displacement ductility ratio, energy-absorbing capability and tensile force values were determined and the failure modes were interpreted from the load-displacement curves obtained as a result of the experiments. It was observed that tensile force and energy-absorbing capacity had increased as a result of installing anchor and applying a tensile force to the concrete that was obtained by adding admixture materials to the reference concrete. In the analytical part of the study, the formulation provided in ACI 318 was used, capacity and design strengths were identified, and their safety levels were determined in comparison with the test results.
Three-year outcomes of ophthalmological examinations performed at 0–3 months of term/preterm infants
Authors: Süt Nurşah Yeniay , Çelik Gökhan , Kızılay Osman , Karatekin Güner , Büyükkayhan Derya 
Published: April 2022

Abstract
Purpose: To present the ocular pathologies detected in babies with gestational ages >34 weeks in the ophthalmology outpatient clinic, as well as determine the frequency of these pathologies and their relationship with various variables, using data covering a span of three years. Materials: The outcomes of the vision screening performed in 7,820 term/preterm infants aged 0–3 months who were examined at the ophthalmology outpatient clinic of Zeynep Kamil Maternity and Children’s Diseases Training and Research Hospital between 2015 and 2017 were analyzed in this retrospective, cross-sectional, observational, and descriptive study. Results: A total of 7,820 term and late-preterm infants were examined. An ocular pathology was identified in 20.1% (n=1,569) of the infants. Retinal hemorrhage was detected as the most common pathology in 9.5% (n=740) of the infants, and it was more common in infants who were ≤28 days than those who were >28 days (p<0.001). Immature vascularization (4.4%; n=346) and conjunctivitis (4.0%; n=313) were the other more common ocular pathologies. Conclusion: Abnormal ocular findings, including disorders that could be components of a systemic disease and could cause loss of vision, were identified in 20.1% of the infants. The first three months of life comprises the period of visual function evolution. Therefore, an ophthalmological examination should be performed on all newborns before discharge or as soon as possible after discharge for the early diagnosis and timely management of abnormal findings.