bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimating the Total Economic Costs of Nutrient Emission Reduction Policies to Halt Eutrophication in the Great Lakes
Authors: Garcia‐Hernandez Jorge A. , Brouwer Roy , Pinto Rute 
Published: April 2022

AbstractThe Great Lakes (GL) in North America are among the largest freshwater resources on the planet facing serious eutrophication problems as a result of excessive nutrient loadings due to population and economic growth. More than a third of Canada's GDP is generated in and around the GL. Hence, the economic interests affected by pollution and pollution control are high. New policies to reduce pollution are often insufficiently informed due to the lack of integrated models and methods that provide decision‐makers insight into the direct and indirect economic impacts of their policies. This study fills this knowledge gap and estimates the impacts of different total phosphorus (TP) restriction policy scenarios across the GL. A first of its kind multi‐regional hydro‐economic model is built for the Canadian GL, extended to include TP emissions from point and non‐point sources. This optimization model is furthermore extended with a pollution abatement cost function that allows sectors to also take technical measures to meet the imposed pollution reduction targets. The latter is a promising new avenue for extending existing hydro‐economic input‐output modeling frameworks. The results show decision‐makers the least cost‐way to achieve different TP emission reduction targets. The estimated cost to reduce TP emissions by 40% in all GL amounts to a total annual cost of 3 billion Canadian dollars or 0.15% of Canada's GDP. The cost structure changes substantially as policy targets become more stringent, increasing the share of indirect costs and affecting not only the economic activities around the GL, but the economy of Canada as a whole due to the tightly interwoven economic structure.
Nuclear Aurora kinase A switches m6A reader YTHDC1 to enhance an oncogenic RNA splicing of tumor suppressor RBM4
Authors: Li SiSi , Qi YangFan , Yu JiaChuan , Hao YuChao , He Bin , Zhang MengJuan , Dai ZhenWei , Jiang TongHui , Li SuYi , Huang Fang , Chen Ning , Wang Jing , Yang MengYing , Liang DaPeng , An Fan , Zhao JinYao , Fan WenJun , Pan YuJia , Deng ZiQian , Luo YuanYuan , Guo Tao , Peng Fei , Hou ZhiJie , Wang ChunLi , Zheng FeiMeng , Xu LingZhi , Xu Jie , Wen QingPing , Jin BiLian , Wang Yang , Liu Quentin 
Published: April 2022

AbstractAberrant RNA splicing produces alternative isoforms of genes to facilitate tumor progression, yet how this process is regulated by oncogenic signal remains largely unknown. Here, we unveil that non-canonical activation of nuclear AURKA promotes an oncogenic RNA splicing of tumor suppressor RBM4 directed by m6A reader YTHDC1 in lung cancer. Nuclear translocation of AURKA is a prerequisite for RNA aberrant splicing, specifically triggering RBM4 splicing from the full isoform (RBM4-FL) to the short isoform (RBM4-S) in a kinase-independent manner. RBM4-S functions as a tumor promoter by abolishing RBM4-FL-mediated inhibition of the activity of the SRSF1-mTORC1 signaling pathway. Mechanistically, AURKA disrupts the binding of SRSF3 to YTHDC1, resulting in the inhibition of RBM4-FL production induced by the m6A-YTHDC1-SRSF3 complex. In turn, AURKA recruits hnRNP K to YTHDC1, leading to an m6A-YTHDC1-hnRNP K-dependent exon skipping to produce RBM4-S. Importantly, the small molecules that block AURKA nuclear translocation, reverse the oncogenic splicing of RBM4 and significantly suppress lung tumor progression. Together, our study unveils a previously unappreciated role of nuclear AURKA in m6A reader YTHDC1-dependent oncogenic RNA splicing switch, providing a novel therapeutic route to target nuclear oncogenic events.
High-temperature phonon-mediated superconductivity in monolayer Mg2B4C2
Authors: Singh Sobhit , Romero Aldo H. , Mella José D. , Eremeev Vitalie , Muñoz Enrique , Alexandrova Anastassia N. , Rabe Karin M. , Vanderbilt David , Muñoz Francisco 
Published: April 2022

AbstractA two-dimensional material – Mg2B4C2, belonging to the family of the conventional superconductor MgB2, is theoretically predicted to exhibit superconductivity with critical temperature Tc estimated in the 47–48 K range (predicted using the McMillian-Allen-Dynes formula) without any tuning of external parameters such as doping, strain, or substrate-induced effects. The origin of such a high intrinsic Tc is ascribed to the presence of strong electron-phonon coupling and large density of states at the Fermi level. This system is obtained after replacing the chemically active boron-boron surface layers in a MgB2 slab by chemically inactive boron-carbon layers. Hence, the surfaces of this material are inert. Our calculations confirm the stability of 2D Mg2B4C2. We also find that the key features of this material remain essentially unchanged when its thickness is increased by modestly increasing the number of inner MgB2 layers.
Identification of gut microbial species linked with disease variability in a widely used mouse model of colitis
Authors: Forster Samuel C. , Clare Simon , Beresford-Jones Benjamin S. , Harcourt Katherine , Notley George , Stares Mark D. , Kumar Nitin , Soderholm Amelia T. , Adoum Anne , Wong Hannah , Morón Bélen , Brandt Cordelia , Dougan Gordon , Adams David J. , Maloy Kevin J. , Pedicord Virginia A. , Lawley Trevor D. 
Published: April 2022

AbstractExperimental mouse models are central to basic biomedical research; however, variability exists across genetically identical mice and mouse facilities making comparisons difficult. Whether specific indigenous gut bacteria drive immunophenotypic variability in mouse models of human disease remains poorly understood. We performed a large-scale experiment using 579 genetically identical laboratory mice from a single animal facility, designed to identify the causes of disease variability in the widely used dextran sulphate sodium mouse model of inflammatory bowel disease. Commonly used treatment endpoint measures—weight loss and intestinal pathology—showed limited correlation and varied across mouse lineages. Analysis of the gut microbiome, coupled with machine learning and targeted anaerobic culturing, identified and isolated two previously undescribed species, Duncaniella muricolitica and Alistipes okayasuensis, and demonstrated that they exert dominant effects in the dextran sulphate sodium model leading to variable treatment endpoint measures. We show that the identified gut microbial species are common, but not ubiquitous, in mouse facilities around the world, and suggest that researchers monitor for these species to provide experimental design opportunities for improved mouse models of human intestinal diseases.
MeadoWatch: a long-term community-science database of wildflower phenology in Mount Rainier National Park
Authors: Manzanedo Rubén D. , John Aji , Sethi Meera L. , Theobald Elli J. , Brosi Berry , Jenkins Joshua , Kloss-Schmidt Ava , Lia Emilia , Schiffer Annie , Sevigny Jordana , Wilson Anna , Yogev Yonit , Hille Ris Lambers Janneke 
Published: April 2022

AbstractWe present a long-term and high-resolution phenological dataset from 17 wildflower species collected in Mt. Rainier National Park, as part of the MeadoWatch (MW) community science project. Since 2013, 457 unique volunteers and scientists have gathered data on the timing of four key reproductive phenophases (budding, flowering, fruiting, and seeding) in 28 plots over two elevational gradients alongside popular park trails. Trained volunteers (87.2%) and University of  Washington scientists (12.8%) collected data 3–9 times/week during the growing season, using a standardized method. Taxonomic assessments were highly consistent between scientists and volunteers, with high accuracy and specificity across phenophases and species. Sensitivity, on the other hand, was lower than accuracy and specificity, suggesting that a few species might be challenging to reliably identify in community-science projects. Up to date, the MW database includes 42,000+ individual phenological observations from 17 species, between 2013 and 2019. However, MW is a living dataset that will be updated through continued contributions by volunteers, and made available for its use by the wider ecological community.
Association between glucokinase regulator gene polymorphisms and serum uric acid levels in Taiwanese adolescents
Authors: Ho Li-Ju , Lu Chieh-Hua , Su Ruei-Yu , Lin Fu-Huang , Su Sheng-Chiang , Kuo Feng-Chih , Chu Nain-Feng , Hung Yi-Jen , Liu Jhih-Syuan , Hsieh Chang-Hsun 
Published: April 2022

AbstractThe glucokinase regulator gene (GCKR) is located on chromosome 2p23. It plays a crucial role in maintaining plasma glucose homeostasis and metabolic traits. Recently, genome-wide association studies have revealed a positive association between hyperuricemia and GCKR variants in adults. This study investigated this genetic association in Taiwanese adolescents. Data were collected from our previous cross-sectional study (Taipei Children Heart Study). The frequencies of various genotypes (CC, CT, and TT) or alleles (C and T) of the GCKR intronic single-nucleotide polymorphism (SNP) rs780094 and the coding SNP rs1260326 (Pro446Leu, a common 1403C-T transition) were compared between a total of 968 Taiwanese adolescents (473 boys, 495 girls) with hyperuricemia or normal uric acid levels on the basis of gender differences. Logistic and linear regression analyses explored the role of GCKR in abnormal uric acid (UA) levels. Boys had higher UA levels than girls (6.68 ± 1.29 and 5.23 ± 0.95 mg/dl, respectively, p < 0.001). The analysis of both SNPs in girls revealed that the T allele was more likely to appear in patients with hyperuricemia than the C allele. After adjusting for confounders, the odds ratio (OR) for hyperuricemia incidence in the TT genotype was 1.75 (95% confidence interval [CI] 1.02–3.00), which was higher than that in the C allele carriers in rs1260326 in the girl population. Similarly, the TT genotypes had a higher risk of hyperuricemia, with an OR of 2.29 (95% CI 1.11–4.73) for rs1260326 and 2.28 (95% CI 1.09–4.75) for rs780094, than the CC genotype in girl adolescents. The T (Leu446) allele of GCKR rs1260326 polymorphism is associated with higher UA levels in Taiwanese adolescent girls.
Classification of pleural effusions using deep learning visual models: contrastive-loss
Authors: Lee Jang Ho , Choi Chang-Min , Park Namu , Park Hyung Jun 
Published: April 2022

AbstractBlood and fluid analysis is extensively used for classifying the etiology of pleural effusion. However, most studies focused on determining the presence of a disease. This study classified pleural effusion etiology employing deep learning models by applying contrastive-loss. Patients with pleural effusion who underwent thoracentesis between 2009 and 2019 at the Asan Medical Center were analyzed. Five different models for categorizing the etiology of pleural effusion were compared. The performance metrics were top-1 accuracy, top-2 accuracy, and micro-and weighted-AUROC. UMAP and t-SNE were used to visualize the contrastive-loss model’s embedding space. Although the 5 models displayed similar performance in the validation set, the contrastive-loss model showed the highest accuracy in the extra-validation set. Additionally, the accuracy and micro-AUROC of the contrastive-loss model were 81.7% and 0.942 in the validation set, and 66.2% and 0.867 in the extra-validation set. Furthermore, the embedding space visualization in the contrastive-loss model exhibited typical and atypical effusion results by comparing the true and false positives of the rule-based criteria. Therefore, classifying the etiology of pleural effusion was achievable using the contrastive-loss model. Conclusively, visualization of the contrastive-loss model will provide clinicians with valuable insights for etiology diagnosis by differentiating between typical and atypical disease types.
TOX4 facilitates promoter-proximal pausing and C-terminal domain dephosphorylation of RNA polymerase II in human cells
Authors: Liu Ziling , Wu Aiwei , Wu Zhen , Wang Talang , Pan Yixuan , Li Bing , Zhang Xumin , Yu Ming 
Published: April 2022

AbstractTOX4 is one of the regulatory factors of PP1 phosphatases with poorly understood functions. Here we show that chromatin occupancy pattern of TOX4 resembles that of RNA polymerase II (Pol II), and its loss increases cellular level of C-terminal domain (CTD) phosphorylated Pol II but mainly decreases Pol II occupancy on promoters. In addition, elongation rate analyses by 4sUDRB-seq suggest that TOX4 restricts pause release and early elongation but promotes late elongation. Moreover, TT-seq analyses indicate that TOX4 loss mainly decreases transcriptional output. Mechanistically, TOX4 may restrict pause release through facilitating CTD serine 2 and DSIF dephosphorylation, and promote Pol II recycling and reinitiation through facilitating CTD serines 2 and 5 dephosphorylation. Furthermore, among the PP1 phosphatases, TOX4 preferentially binds PP1α and is capable of facilitating Pol II CTD dephosphorylation in vitro. These results lay the foundation for a better understanding of the role of TOX4 in transcriptional regulation.
A benzodiazepine activator locks Kv7.1 channels open by electro-mechanical uncoupling
Authors: Schreiber Julian A. , Möller Melina , Zaydman Mark , Zhao Lu , Beller Zachary , Becker Sebastian , Ritter Nadine , Hou Panpan , Shi Jingyi , Silva Jon , Wrobel Eva , Strutz-Seebohm Nathalie , Decher Niels , Schmitt Nicole , Meuth Sven G. , Düfer Martina , Wünsch Bernhard , Cui Jianmin , Seebohm Guiscard 
Published: April 2022

AbstractLoss-of-function mutations in Kv7.1 often lead to long QT syndrome (LQTS), a cardiac repolarization disorder associated with arrhythmia and subsequent sudden cardiac death. The discovery of agonisticIKsmodulators may offer a new potential strategy in pharmacological treatment of this disorder. The benzodiazepine derivative (R)-L3 potently activates Kv7.1 channels and shortens action potential duration, thus may represent a starting point for drug development. However, the molecular mechanisms underlying modulation by (R)-L3 are still unknown. By combining alanine scanning mutagenesis, non-canonical amino acid incorporation, voltage-clamp electrophysiology and fluorometry, andin silicoprotein modelling, we show that (R)-L3 not only stimulates currents by allosteric modulation of the pore domain but also alters the kinetics independently from the pore domain effects. We identify novel (R)-L3-interacting key residues in the lower S4-segment of Kv7.1 and observed an uncoupling of the outer S4 segment with the inner S5, S6 and selectivity filter segments.
Efficient valley polarization of charged excitons and resident carriers in Molybdenum disulfide monolayers by optical pumping
Authors: Park Sangjun , Arscott Steve , Taniguchi Takashi , Watanabe Kenji , Sirotti Fausto , Cadiz Fabian 
Published: April 2022

AbstractThe roadmap of future innovative device developments foresees the reduction of material dimensions down to nanometer scale and the incorporation of novel degrees of freedom. For instance, electrons and holes in 2D semiconductors like MoS2 monolayers exhibit a unique coupling between the spin and the crystal momentum, also referred to as the valley. A crucial requirement for future applications is therefore the possibility to initialise the spin/valley degree of freedom in these materials. Here we investigate the optical initialisation of the valley degree of freedom in charge-tunable MoS2 monolayers encapsulated with hexagonal boron nitride at cryogenic temperatures. We report in photoluminescence a large steady state valley polarization of the different excitonic complexes following circularly-polarized laser excitation. We reveal efficient valley initialisation of positively-charged excitons, which have so far proved to be elusive in non-encapsulated monolayers due to defect and laser-induced large electron doping. We find that negatively-charged excitons present a polarization of 70% which is unusually large for non-resonant excitation. We attribute this large valley polarization to the particular band structure of MoS2. In addition, we demonstrate that circular excitation induces a dynamical polarization of resident electrons and holes––as recently shown in tungsten-based monolayers.
Black carbon emissions from traffic contribute substantially to air pollution in Nairobi, Kenya
Authors: Kirago Leonard , Gatari Michael J. , Gustafsson Örjan , Andersson August 
Published: April 2022

AbstractRapid urbanization and population growth drives increased air pollution across Sub-Saharan Africa with serious implications for human health, yet pollutant sources are poorly constrained. Here, we analyse fine particulate aerosol concentrations and radiocarbon composition of black carbon over a full annual cycle in Nairobi, Kenya. We find that particle concentrations exceed the World Health Organisation’s recommended safe limit throughout the year, with little seasonal variability in particle concentration or composition. Organics (49 ± 7%) and water-soluble inorganic ions, dominated by sulfates (13 ± 5%), constitute the largest contributors to the particle loadings. Unlike large cities on other continents, the fraction of black carbon in particles is high (15 ± 4%) suggesting black carbon is a prominent air pollutant in Nairobi. Radiocarbon-based source quantification indicates that fossil fuel combustion emissions are a dominant source of black carbon throughout the year (85 ± 3%). Taken together, this indicates that black carbon emissions from traffic are a key stressor for air quality in Nairobi.
Patterns and frequency of projected future tropical cyclone genesis are governed by dynamic effects
Authors: Murakami Hiroyuki , Wang Bin 
Published: April 2022

AbstractPotential future changes in the genesis frequency and distribution of tropical cyclones are important for society, yet uncertain. Confidence in the model projections largely relies on whether we can physically explain why the models projected such changes. Here we analyze multi-model climate simulations, and find that future changes in the patterns and frequency of tropical cyclone genesis are largely governed by dynamic effects—that is, by human-induced changes in the atmospheric circulation. These large-scale circulation changes include decreases in the mid-level upward motion and lower-to-mid level cyclonic vorticity, and increases in vertical wind shear. Conversely, the thermodynamic effect—a result of increased maximum potential intensity in a warmer climate—would yield tropical cyclone genesis patterns that are opposite to the model projections. We conclude that dynamic changes in response to anthropogenic greenhouse gas emissions are an important factor in determining the response of tropical cyclones to global warming.
Widespread subsonic turbulence in Ophiuchus North 1
Authors: Gong Yan , Liu Shu , Wang Junzhi , Zhu Weishan , Li Guang-Xing , Yang Wenjin , Sun Jixian 
Published: April 2022

Context. Supersonic motions are common in molecular clouds. (Sub)sonic turbulence is usually detected toward dense cores and filaments. However, it remains unknown whether (sub)sonic motions at larger scales (≳1 pc) may be present in various environments.
Aims. Located at a distance of about 110 pc, Ophiuchus North 1 (Oph N1) is one of the nearest molecular clouds that would allow for an in-depth investigation of its turbulence properties via large-scale mapping observations of single-dish telescopes.
Methods. We carried out the 12CO (J = 1−0) and C18O (J = 1−0) imaging observations toward Oph N1 with the Purple Mountain Observatory 13.7 m telescope. The observations have an angular resolution of ~55″ (i.e., 0.03 pc).
Results. Most of the whole C18O emitting regions have Mach numbers of ≲1, demonstrating the large-scale (sub)sonic turbulence across Oph N1. Based on the polarization measurements, we estimate the magnetic field strength of the plane-of-sky component to be ≳9 µG. We infer that Oph N1 is globally sub-Alfvénic, and is supported against gravity mainly by the magnetic field. The steep velocity structure function can be caused by the expansion of the Sh 2–27 HII region or the dissipative range of incompressible turbulence.
Conclusions. Our observations reveal a surprising case of clouds that are characterized by widespread subsonic turbulence and a steep relation between the size and the linewidth. This cloud is magnetized where ion-neutral friction is assumed to play an important role.
Outcomes of double level osteotomy for osteoarthritic knees with severe varus deformity. A systematic review
Authors: Elbardesy Hany , McLeod André , Ghaith Hazem S. , Hakeem Samir , Housden Philip 
Published: April 2022

Background: When correcting severe genu varus deformity, knee surgeons must choose between performing a single or double-level osteotomy. This systematic review aims to provide this equipoise with some clarity. Patients and methods: We conducted this study following the Preferred Reporting Items for Systematic Reviews and Meta-analyses Statement (PRISMA) and the Cochrane Handbook for systematic reviews and meta-analysis. Studies evaluating the effect of the double level osteotomy (DLO) or those comparing it to high tibial osteotomy (HTO) from all regions and written in any language were included. Results: Six studies were included in this systematic review. They were prepared and analysed using Review Manager V5.0 [Computer Program] (RevMan5). Performing DLO resulted in restoring patellar height, joint-line convergence angle (JLCA), and mMPTA to normal values. DLO was also more successful at avoiding joint line obliquity (JLO) in severe varus deformity when compared to HTO (P < 0.001). No significant difference was reported between the two cohorts regarding the mLPTA. DLO resulted in satisfactory short term KOOS and IKDC scores. The complication rate after DLO was 2.28%. Conclusions: DLO showed a low complication rate and satisfactory short term KOOS and IKDAC scores. Randomised control trials with long term follow-up comparing the DLO and HTO are recommended.
Ultrafast decay dynamics of 2-hydroxypyridine excited to S1 electronic state
Authors: Min Yan-jun , Yuan Wen-peng , Yang Dong-yuan , Dai Dong-xu , Yu Sheng-rui , Wu Guo-rong , Yang Xue-ming 
Published: April 2022

The S1 state decay dynamics of 2-hydroxypyridine following UV excitation at a wavelength range of 276.9−250.0 nm is investigated using femtosecond time-resolved photoelectron imaging technique. Based on pump wavelength dependence of the decay dynamics, a refined decay picture is proposed. At pump wavelength of 276.9 nm, the S1 state is depopulated through intersystem crossing to lower triplet state(s). At 264.0 nm, both intersystem crossing to lower triplet state(s) and internal conversion to the ground state are in operation. At 250.0 nm, internal conversion to the ground state becomes dominated.
Matrix Inversion and Subset Selection (MISS): A pipeline for mapping of diverse cell types across the murine brain
Authors: Mezias Christopher , Torok Justin , Maia Pedro D. , Markley Eric , Raj Ashish 
Published: April 2022

Significance
The current state-of-the-art mappings of cell types fall short regarding finely resolved subtypes of neural cells, especially γ-aminobutyric acidergic and glutamatergic subtypes. Most such maps compromise on either the number or specificity of unique cell types quantified in each study. Others only use qualitative validation for their maps and fail to address whether gene subset selection is necessary for optimal maps. The Matrix Inversion and Subset Selection pipeline uses publicly available in situ hybridization and single-cell RNA sequencing gene expression data to infer cell-type distributions to map diverse cell types across the murine brain. Most importantly, we demonstrate that data-driven feature selection is necessary to arrive at quantitatively optimal cell-type maps using inversion-, deconvolution-, and correlation-based mapping approaches.