bluuebunny/crossref_metadata_2025_split huggingface dataset:
Data fusion of electronic noses and electronic tongues aids in botanical origin identification on imbalanced Codonopsis Radix samples
Authors: Wang Shuying , Lin Zhaozhou , Zhang Bei , Du Jing , Li Wen , Wang Zhibin 
Published: November 2022

AbstractCodonopsis Radix (CR) is an edible food and traditional Chinese herb medicine in China. Various varieties of Codonopsis Radix have different tastes. To make the flavor of processed food stable, two kinds of electronic sensory devices, electronic nose and electronic tongue, were used to establish a discrimination model to identify the botanical origin of each sample. The optimal model built on the 88 batches of samples was selected from the models trained with all combination of two pretreatment methods and three classification methods. A comparison were performed on the models trained on the data collected by electronic nose and electronic tongue. The results showed that the model trained on the fused dataset outperformed the models trained separately on the electronic nose data and electronic tongue data. The two preprocessing approaches could improve the prediction performance of all classification methods. Classification and Regression Tree approach performed better than Partial Least Square Discriminant Analysis and Linear Discriminant Analysis in terms of accuracy. But Classification and Regression Tree tends to assign the samples of minority class to the majority class. Meanwhile, Partial Least Square Discriminant Analysis keeps a good balance between the identification requirements of all the two groups of samples. Taking all the results above, the model built using the Partial Least Square Discriminant Analysis method on the fused data after z-score was used to identify the botanical origin of Codonopsis Radix.
Machine learning for morbid glomerular hypertrophy
Authors: Ushio Yusuke , Kataoka Hiroshi , Iwadoh Kazuhiro , Ohara Mamiko , Suzuki Tomo , Hirata Maiko , Manabe Shun , Kawachi Keiko , Akihisa Taro , Makabe Shiho , Sato Masayo , Iwasa Naomi , Yoshida Rie , Hoshino Junichi , Mochizuki Toshio , Tsuchiya Ken , Nitta Kosaku 
Published: November 2022

AbstractA practical research method integrating data-driven machine learning with conventional model-driven statistics is sought after in medicine. Although glomerular hypertrophy (or a large renal corpuscle) on renal biopsy has pathophysiological implications, it is often misdiagnosed as adaptive/compensatory hypertrophy. Using a generative machine learning method, we aimed to explore the factors associated with a maximal glomerular diameter of ≥ 242.3 μm. Using the frequency-of-usage variable ranking in generative models, we defined the machine learning scores with symbolic regression via genetic programming (SR via GP). We compared important variables selected by SR with those selected by a point-biserial correlation coefficient using multivariable logistic and linear regressions to validate discriminatory ability, goodness-of-fit, and collinearity. Body mass index, complement component C3, serum total protein, arteriolosclerosis, C-reactive protein, and the Oxford E1 score were ranked among the top 10 variables with high machine learning scores using SR via GP, while the estimated glomerular filtration rate was ranked 46 among the 60 variables. In multivariable analyses, the R2 value was higher (0.61 vs. 0.45), and the corrected Akaike Information Criterion value was lower (402.7 vs. 417.2) with variables selected with SR than those selected with point-biserial r. There were two variables with variance inflation factors higher than 5 in those using point-biserial r and none in SR. Data-driven machine learning models may be useful in identifying significant and insignificant correlated factors. Our method may be generalized to other medical research due to the procedural simplicity of using top-ranked variables selected by machine learning.
A bead-based GPCR phosphorylation immunoassay for high-throughput ligand profiling and GRK inhibitor screening
Authors: Kaufmann Johanna , Blum Nina Kathleen , Nagel Falko , Schuler Anna , Drube Julia , Degenhart Carsten , Engel Julian , Eickhoff Jan Eicke , Dasgupta Pooja , Fritzwanker Sebastian , Guastadisegni Maria , Schulte Clemens , Miess-Tanneberg Elke , Maric Hans Michael , Spetea Mariana , Kliewer Andrea , Baumann Matthias , Klebl Bert , Reinscheid Rainer K. , Hoffmann Carsten , Schulz Stefan 
Published: November 2022

AbstractAnalysis of agonist-driven phosphorylation of G protein-coupled receptors (GPCRs) can provide valuable insights into the receptor activation state and ligand pharmacology. However, to date, assessment of GPCR phosphorylation using high-throughput applications has been challenging. We have developed and validated a bead-based immunoassay for the quantitative assessment of agonist-induced GPCR phosphorylation that can be performed entirely in multiwell cell culture plates. The assay involves immunoprecipitation of affinity-tagged receptors using magnetic beads followed by protein detection using phosphorylation state-specific and phosphorylation state-independent anti-GPCR antibodies. As proof of concept, five prototypical GPCRs (MOP, C5a1, D1, SST2, CB2) were treated with different agonizts and antagonists, and concentration-response curves were generated. We then extended our approach to establish selective cellular GPCR kinase (GRK) inhibitor assays, which led to the rapid identification of a selective GRK5/6 inhibitor (LDC8988) and a highly potent pan-GRK inhibitor (LDC9728). In conclusion, this versatile GPCR phosphorylation assay can be used extensively for ligand profiling and inhibitor screening.
Self‐inferring incomplete multi‐view clustering
Authors: Fan Junjun , Ma ZeQi , Wen Jiajun , Lai Zhihui , Xie Weicheng , Wong Wai Keung 
Published: November 2022

AbstractWith the advantage of exploiting complementary and consensus information across multiple views, techniques for Multi‐view Clustering have attracted increasing attention in recent years. However, it is common that data on some views is not completed in real‐world applications, which brings the challenge of partial mapping between the views. To explore the information hidden in the local geometric structure and recover missing instances through mining the information hidden in existing instances, a self‐inferring incomplete multi‐view clustering algorithm is proposed. Firstly, the incomplete multi‐view data is replenished directly and exploited as variables for inferring the missing instances. And then, a feature graph constraint is united in consensus learning. Besides, a similarity graph learning method is imposed to preserve the local manifold structure. At last, the inferred instances are filled in the missing instances for learning better consensus representation in the iterative process. Extensive experiment results show that this method can improve the clustering performance compared with the state‐of‐the‐art methods.
Disentangling the protoplanetary disk gas mass and carbon depletion through combined atomic and molecular tracers
Authors: Sturm J. A. , Booth A. S. , McClure M. K. , Leemker M. , van Dishoeck E. F. 
Published: November 2022

Context. The total disk gas mass and elemental C, N, and O composition of protoplanetary disks are crucial ingredients for improving our understanding of planetary formation. Measuring the gas mass is complicated, since H2 cannot be detected in the cold bulk of the disk and the elemental abundances with respect to hydrogen are degenerate with gas mass in all disk models.
Aims. We aim to determine the gas mass and elemental abundances ratios C/H and O/H in the transition disk around LkCa 15, one of the few disks for which HD data are available, in combination with as many chemical tracers as possible.
Methods. We present new NOrthern Extended Millimeter Array observations of CO, 13CO, C18O, and optically thin C17O J = 2−1 lines, along with high angular-resolution Atacama Large Millimeter Array millimeter continuum and CO data to construct a representative model of LkCa 15. Using a grid of 60 azimuthally symmetric thermo-chemical DALI disk models, we translated the observed fluxes to elemental abundances and constrained the best-fitting parameter space of the disk gas mass.
Results. The transitions that constrain the gas mass and carbon abundance the most are C17O J = 2−1, N2H+ J = 3−2 and HD J = 1−0. Using these three molecules, we find that the gas mass in the LkCa 15 disk is Mg = 0.01−0.004+0.01 M⊙, which is a factor of 6 lower than previous estimations. This value is consistent with cosmic ray ionization rates between 10−16−10−18 s−1, where 10−18 s−1 is a lower limit based on the HD upper limit. The carbon abundance is C/H = (3 ± 1.5) × 10−5, implying a moderate depletion of elemental carbon by a factor of 3–9. All other analyzed transitions also agree with these numbers, within a modeling uncertainty of a factor of 2. Using the resolved C2H image we find a C/O ratio of ~1, which is consistent with literature values of H2O depletion in this disk. The absence of severe carbon depletion in the LkCa 15 disk is consistent with the young age of the disk, but stands in contrast to the higher levels of depletion seen in older cold transition disks.
Conclusions. Combining optically thin CO isotopologue lines with N2H+ is promising with regard to breaking the degeneracy between gas mass and CO abundance. The moderate level of depletion for this source with a cold, but young disk, suggests that long carbon transformation timescales contribute to the evolutionary trend seen in the level of carbon depletion among disk populations, rather than evolving temperature effects and presence of dust traps alone. HD observations remain important for determining the disk’s gas mass.
New strong lensing modelling of SDSS J2222+2745 enhanced with VLT/MUSE spectroscopy
Authors: Acebron A. , Grillo C. , Bergamini P. , Caminha G. B. , Tozzi P. , Mercurio A. , Rosati P. , Brammer G. , Meneghetti M. , Nonino M. , Vanzella E. 
Published: November 2022

Context.SDSS J2222+2745, atz = 0.489, is one of the few currently known lens clusters with multiple images (six) of a background (z = 2.801) quasar with measured time delays between two image pairs (with a sub-percent relative error for the longer time delay). Systems of this kind can be exploited as alternative cosmological probes through high-precision and accurate strong lensing models.Aims.We present recent observations from the Multi Unit Spectroscopic Explorer (MUSE) on the Very Large Telescope (VLT) and new total mass models of the core of the galaxy cluster SDSS J2222+2745.Methods.We combine archival multi-band, high-resolution imaging from theHubbleSpace Telescope (HST) with our VLT/MUSE spectroscopic data to securely identify 34 cluster members and 12 multiple images from 3 background sources. We also measure the stellar velocity dispersions of 13 cluster galaxies, down to HSTF160W= 21 mag, enabling an independent estimate of the contribution of the sub-halo mass component to the lens total mass. By leveraging the new spectroscopic dataset, we build improved strong lensing models.Results.The projected total mass distribution of the lens cluster is best modelled with a single large-scale mass component, a galaxy-scale component, anchored by the VLT/MUSE kinematic information, and an external shear component. The best-fit strong lensing model yields a root mean square separation between the model-predicted and observed positions of the multiple images of 0″​​.29. When analysing the impact of systematic uncertainties, stemming from modelling assumptions and used observables, we find that the resulting projected total mass profile, the relative weight of the sub-halo mass component, and the critical lines are consistent, within the statistical uncertainties. The predicted magnification and time-delay values are, instead, more sensitive to the local details of the lens total mass distribution, and vary significantly among lens models that are similarly good at reproducing the observed multiple image positions. In particular, the model-predicted time delays can differ by a factor of up to ∼1.5.Conclusions.SDSS J2222+2745 is a promising lens cluster for cosmological applications. However, due to its complex morphology, the relatively low number of secure ‘point-like’ multiple images, and current model degeneracies, it becomes clear that additional information (from the observed surface brightness distribution of lensed sources and the measured time delays) needs to be included in the modelling for accurate and precise cosmological measurements. The full VLT/MUSE secure spectroscopic catalogue presented in this work is made publicly available.
On K−-nuclear interaction, K−-nuclear quasibound states and K− atoms
Authors: Óbertová Jaroslava , Friedman Eliahu , Mareš Jiří , Ramos Àngels 
Published: November 2022

The K−N potentials derived from SU(3) chiral coupled-channel meson-baryon interaction models fail to describe kaonic atom data. It was shown that K− single-nucleon potentials have to be supplemented by a phenomenological K− multi-nucleon optical potential in order to achieve good description of the data. Moreover, only the Prague, Kyoto-Munich and Barcelona models are able to account for the experimental data on K− atoms and K− singlenucleon absorption ratios from bubble chamber experiments. A considerable imaginary part coming from the multi-nucleon absorption ruled out existence of narrow K−-nuclear quasibound states in nuclei with A ≥ 6.
We have developed a microscopic model for the K−NN absorption in nuclear matter. The absorption was described as a meson-exchange process and the primary K−N interaction strength was derived from the state-of-the-art chiral models. The medium modifications of the K−N scattering amplitudes due to the Pauli exclusion principle were taken into account. The model was applied in calculations of kaonic atoms for the first time. The description of the data improved significantly when the two nucleon absorption was considered.
Identification and expression profile of odorant-binding proteins in the parasitic wasp Microplitis pallidipes using PacBio long-read sequencing
Authors: Zhang Hao , Wang Jin-Yan , Wan Nian-Feng , Chen Yi-Juan , Ji Xiang-Yun , Jiang Jie-Xian 
Published: November 2022

Microplitis pallidipes Szépligeti (Hymenoptera: Braconidae) is an important parasitic wasp of second and third-instar noctuid larvae such as the insect pests Spodoptera exigua, Spodoptera litura, and Spodoptera frugiperda. As in other insects, M. pallidipes has a chemosensory recognition system that is critical to foraging, mating, oviposition, and other behaviors. Odorant-binding proteins (OBPs) are important to the system, but those of M. pallidipes have not been determined. This study used PacBio long-read sequencing to identify 170,980 M. pallidipes unigenes and predicted 129,381 proteins. Following retrieval of possible OBP sequences, we removed those that were redundant or non-full-length and eventually cloned five OBP sequences: MpOBP2, MpOBP3, MpOBP8, MpOBP10, and MpPBP 429, 429, 459, 420, and 429 bp in size, respectively. Each M. pallidipes OBP had six conserved cysteine residues. Phylogenetic analysis revealed that the five OBPs were located at different branches of the phylogenetic tree. Additionally, tissue expression profiles indicated that MpOBP2 and MpPBP were mainly expressed in the antennae of male wasps, while MpOBP3, MpOBP8, and MpOBP10 were mainly expressed in the antennae of female wasps. MpOBP3 was also highly expressed in the legs of female wasps. Temporal profiles revealed that the expression of each M. pallidipes OBP peaked at different days after emergence to adulthood. In conclusion, we identified five novel odorant-binding proteins of M. pallidipes and demonstrated biologically relevant differences in expression patterns.
Risk Factors of Preterm Birth in Women After Local Treatment of Cervical Intraepithelial Neoplasia – a Retrospective Cohort Study
Authors: Stubert Johannes , Stratmann Elisa , Gerber Bernd , Mann Ellen 
Published: November 2022

Abstract
Purpose A previous cervical intraepithelial neoplasia is associated with an increased obstetrical risk. It was the aim of the study to identify risk factors of preterm birth in
patients with cervical intraepithelial neoplasia in dependence of the treatment modality (excisional vs. ablative).
Methods Women with treated cervical intraepithelial neoplasia and subsequent pregnancy (n = 155) were included in this retrospective study. Methods of treatment were either
conization by large loop excision of the transformation zone (LLETZ) or ablative laser vaporization.
Results Of the total population 60.6% (n = 94) had a conization and 39.4% (n = 61) a laser vaporization alone. The frequency of preterm birth < 37 weeks was 9.7% (n = 15) without
differences between conization and laser (11.7 vs. 6.7%, p = 0.407) with an odds ratio (OR) of 1.9 (95% confidence interval [CI] 0.6–6.2). Preterm birth < 34 weeks was found in 2.6%
(n = 4), of which all had a conization (4.3 vs. 0%, p = 0.157). Risk factors for preterm birth were repeated cervical intervention (OR 4.7 [95% CI 1.5–14.3]), especially a combination of
conization and laser ablation (OR 14.9 [95% CI 4.0–55.6]), age at intervention < 30 years (OR 6.0 [95% CI 1.3–27.4]), a history of preterm birth (OR 4.7 [95% CI 1.3–17.6]) and age at
delivery < 28 years (OR 4.7 [95% CI 1.5–14.3]).
Conclusion The large loop excision of the transformation zone as a modern, less invasive ablative treatment did not obviously increase the risk of preterm birth compared to laser
vaporization. The most important risk factor for preterm delivery was the need of a repeated intervention, especially at younger age. We assume that the persistence or recurrence of the
cervical intraepithelial neoplasia following a high-risk human papillomavirus infection is mainly responsible for the observed effect.
Entwicklung von Praxisempfehlungen für die Soziale Arbeit in
der medizinischen Rehabilitation
Authors: Lukasczik Matthias , Zerban Nina Luisa , Wolf Hans Dieter , Neuderth Silke , Vogel Heiner 
Published: November 2022

Zusammenfassung
Ziele Die Soziale Arbeit ist ein wesentlicher Bestandteil des
Behandlungsspektrums in der medizinischen Rehabilitation. Neue Anforderungen an
ihre Leistungen und Variationen in der praktischen Umsetzung von Interventionen
führen zu einem Bedarf an Versorgungsstandards guter klinischer Praxis
für dieses Feld. Daher wurden Praxisempfehlungen (PE) für die
Soziale Arbeit in medizinischen Rehabilitationseinrichtungen entwickelt. Diese
sollen Entscheidungsprozesse unterstützen, konkrete
Handlungsempfehlungen liefern und anderen Reha-Professionen Einblick in das
Aufgabenspektrum der Sozialen Arbeit geben. Die PE wurden in einem mehrstufigen
Verfahren unter Einbezug von Expert*innen erarbeitet.
Methodik Eine nationale und internationale Literaturrecherche wurde zum
aktuellen Evidenzstand zu Leistungen der Sozialen Arbeit in der medizinischen
Rehabilitation durchgeführt. Darauf folgte eine Befragung der
Sozialdienste in stationären und ambulanten medizinischen
Rehabilitationseinrichtungen (somatische und psychosomatische Indikationen ohne
Abhängigkeitserkrankungen, Kinder-/Jugendlichenrehabilitation;
Nmax=311) zu Vorgehensweisen der Sozialen Arbeit und
Erwartungen an die PE. Auf dieser Basis wurde eine erste Version im Rahmen eines
Expert*innenworkshops weiterentwickelt und in einer zweiten Befragung
durch Sozialdienste in Rehabilitationseinrichtungen evaluiert
(Nmax=184). Die Ergebnisse wurden auf einem zweiten Workshop
diskutiert, auf dieser Grundlage wurde eine endgültige Fassung der PE
erstellt.
Ergebnisse Die PE umfassen Informationen zu Rahmenbedingungen der
Tätigkeit Sozialer Arbeit in der Rehabilitation und zur Sozialen
Diagnostik. Im Hauptteil werden häufige Bedarfslagen von
Rehabilitand*innen dargestellt: berufliche Bedarfslagen (u. a.
längere Abwesenheit vom Arbeitsplatz), soziale und wirtschaftliche
Bedarfslagen (u. a. Teilhabe von Menschen mit Behinderung); Bedarf an
nachgehenden Leistungen (u. a. Nachsorge) und Bedarfslagen im Bereich
der Kommunikation. Aufgeführt sind jeweils Informationen zur
Feststellung der Bedarfslage, zu Inhalt und Umfang von Leistungen und Verweise
auf weiterführende Materialien.
Schlussfolgerungen Die PE sollen als Praxishilfe, die sowohl auf den
praktischen Erfahrungen aus Rehabilitationseinrichtungen als auch auf
vorhandener wissenschaftlicher Evidenz beruht, Mitarbeiter*innen der
Sozialen Arbeit und anderen Berufsgruppen in der Rehabilitation Informationen zu
Leistungen der Sozialen Arbeit und deren Umsetzung bieten. Die inhaltliche
Schwerpunktsetzung spiegelt die geäußerten Erwartungen der
Sozialdienste an die PE wider.
Besonderheiten bei der Therapie von Klavikulafrakturen
Authors: Müller Christof A. 
Published: November 2022

ZusammenfassungDie Bedeutung der operativen im Gegensatz zur konservativen Behandlung der Klavikulafraktur hat in den letzten Jahren deutlich zugenommen. Minimalinvasive Verfahren, wie die Osteosynthese
mittels TEN und neue Plattenformen in Kombination mit der Winkelstabilität, gewährleisten sichere Stabilisierungen mit dem Vorteil der Frühmobilisation. Dennoch hat die konservative Therapie
ihren Stellenwert bei wenig dislozierten Frakturen und älteren, multimorbiden Patienten. Der Patient ist somit individuell in die Entscheidung mit einzubeziehen. Absolute Indikationen für
die operative Therapie sind selten. Nur bei offenen Frakturen oder drohender Perforation sowie neurovaskulären Begleitverletzungen besteht eine absolute OP-Indikation. Einfache Frakturformen
können gut minimalinvasiv mittels TEN versorgt werden. Bei mehrfragmentären Frakturformen ist die Plattenosteosynthese Mittel der Wahl, um eine sichere Stabilisierung ohne Implantatwanderung
zu gewährleisten.
Arthroskopisch assistierte Frakturversorgung, was ist möglich und sinnvoll?
Authors: Culemann Ulf , Tatour Anton 
Published: November 2022

ZusammenfassungDie Arthroskopie ist ein probates Mittel zur Diagnostik und Therapie intraartikulärer Verletzungen. Was ursprünglich den großen Gelenken wie Knie und Schulter vorbehalten war, hat
inzwischen in vielen weiteren, auch kleineren Gelenken Einzug gehalten. Zudem ist die Arthroskopie auch ein wichtiges Instrument zur intraartikulären Kontrolle bei der Reposition
von Gelenkfrakturen. So sind arthroskopisch assistierte Frakturversorgungen vom Azetabulum bis hin zum oberen Sprunggelenk bereits beschrieben. Insbesondere bei der
Frakturversorgung der Schulterregion haben in den vergangenen Jahren zunehmend minimalinvasive, rein arthroskopische und Hybridverfahren (teils offen – teils arthroskopisch) Einzug
gehalten. Die arthroskopische Unterstützung geht sogar bis zur kompletten Implantatentfernung von eingebrachten Plattenosteosynthesen nach verheilter proximaler Oberarmfraktur.
Ziel der vorliegenden Arbeit soll es vornehmlich sein, den allgemeinen Stand der arthroskopischen Behandlungsmöglichkeiten im Bereich der Schulter beispielhaft aufzuzeigen und ein
in unserer Klinik häufig angewendetes Verfahren anhand eines Fallbeispiels darzustellen.