bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of COVID-19 pandemic on hospital admissions for nine diseases in Iran: insight from an interrupted time series analysis
Authors: Ahmadi Sina , Kazemi-Karyani Ali , Badiee Nasim , Byford Sarah , Mohammadi Ali , Piroozi Bakhtiar , Rezaei Satar 
Published: November 2022

Abstract
Background
Associations between the COVID-19 pandemic and hospitalizations have not been studied Iran. This study aimed to examine the impact of the COVID-19 pandemic on hospital admissions for nine categories of disease in seven public hospitals in Kermsnahah city, the capital of Kermsnahah province, in the west of Iran.

Methods
Data on monthly hospitalization rates (number of hospitalizations per 100,000 population) were collected for nine categories of disease for a period of 40 months (23 months before and 17 months after the COVID-19 outbreak in Iran) from the health information systems of all seven public hospitals in Kermanshah city. Categories of disease included those related to pregnancy, childbirth and the puerperium period, neoplasms, diseases of the digestive, respiratory, circulatory, genitourinary and nervous systems, mental and behavioural disorders, and infectious and parasitic diseases. Population data were extracted from the Statistics Centre of Iran. An interrupted time series analysis with segmented regression was used to examine the impact of COVID-19 on hospital admissions.

Findings
Average monthly hospitalization rates fell for all nine categories of disease included in the study after the onset of the pandemic, with overall rates of 85.5 per 100,000 population in the period before the COVID-19 outbreak and 50.4 per 100,000 population after the outbreak began. The relative reduction in hospitalizations for the nine diseases was 56.4%. Regression analysis of monthly data indicated a sharp decrease in hospitalisations during the first month after the COVID-19 outbreak, which was statistically significant for all diseases (p < 0.001). After the initial reduction following onset of the pandemic, significant increases were observed for some diseases, including neoplasms (increase of 3.17 per 100,000 population; p < 0.001), diseases of the digestive system (increase of 1.17 per 100,000 population; p < 0.001) and diseases related to pregnancy, childbirth and the puerperium period (increase of 1.73 per 100,000 population). For other categories of disease, rates significantly declined, including infectious and parasitic diseases (decrease of 2.46 per 100,000 population; p < 0.001). Hospitalization rates did not increase to pre-pandemic levels for any disease, with the exception of those related to pregnancy, childbirth and the puerperium period.

Conclusions
Our study indicated that the COVID-19 pandemic had a significantly negative effect on hospitalizations in Iran. Although use of hospital care has gradually increased post-outbreak, it has yet to return to normal levels.
Diagnostic utility of CSF1 immunohistochemistry in tenosynovial giant cell tumor for differentiating from giant cell-rich tumors and tumor-like lesions of bone and soft tissue
Authors: Sugita Shintaro , Takenami Tomoko , Kido Tomomi , Aoyama Tomoyuki , Hosaka Michiko , Segawa Keiko , Sugawara Taro , Fujita Hiromi , Shimizu Junya , Murahashi Yasutaka , Emori Makoto , Hasegawa Tadashi 
Published: November 2022

Abstract
Background
Tenosynovial giant cell tumor (TSGCT) is a benign fibrohistiocytic tumor that affects the synovium of joints, bursa, and tendon sheaths and is categorized into localized TSGCT (LTSGCT) and diffuse TSGCT (DTSGCT). LTSGCT and DTSGCT are characterized by recurrent fusions involving the colony-stimulating factor 1 (CSF1) gene and its translocation partner collagen type VI alpha 3 chain. The fusion gene induces intratumoral overexpression of CSF1 mRNA and CSF1 protein. CSF1 expression is a characteristic finding of TSGCT and detection of CSF1 mRNA and CSF1 protein may be useful for the pathological diagnosis. Although there have been no effective anti-CSF1 antibodies to date, in situ hybridization (ISH) for CSF1 mRNA has been performed to detect CSF1 expression in TSGCT. We performed CSF1 immunohistochemistry (IHC) using anti-CSF1 antibody (clone 2D10) in cases of TSGCT, giant cell-rich tumor (GCRT), and GCRT-like lesion and verified its utility for the pathological diagnosis of TSGCT.

Methods
We performed CSF1 IHC in 110 cases including 44 LTSGCTs, 20 DTSGCTs, 1 malignant TSGCT (MTSGCT), 10 giant cell tumors of bone, 2 giant cell reparative granulomas, 3 aneurysmal bone cysts, 10 undifferentiated pleomorphic sarcomas, 10 leiomyosarcomas, and 10 myxofibrosarcomas. We performed fluorescence ISH (FISH) for CSF1 rearrangement to confirm CSF1 expression on IHC in TSGCTs. We considered the specimens to have CSF1 rearrangement if a split signal was observed in greater than 2% of the tumor cells.

Results
Overall, 50 of 65 TSGCT cases, including 35 of the 44 LTSGCTs and 15 of the 20 DTSGCTs, showed distinct scattered expression of CSF1 in the majority of mononuclear tumor cells. MTSGCT showed no CSF1 expression. Non-TSGCT cases were negative for CSF1. FISH revealed CSF1 rearrangement in 6 of 7 CSF1-positive cases on IHC. On the other hand, FISH detected no CSF1 rearrangement in all CSF1-negative cases on IHC. Thus, the results of IHC corresponded to those of FISH.

Conclusion
We revealed characteristic CSF1 expression on IHC in cases of TSGCT, whereas the cases of non-TSGCT exhibited no CSF1 expression. CSF1 IHC may be useful for differentiating TSGCTs from histologically mimicking GCRTs and GCRT-like lesions.
Label-free detection of leukemic myeloblasts in hyaluronic acid
Authors: Park Suhyun , Kim Hyueyun , Woo Minna , Kim Minsuk 
Published: November 2022

AbstractChronic myeloid leukemia is generally required bone marrow biopsy for diagnosis. Although examining peripheral blood is less invasive, it has not been fully validated as a routine diagnostic test due to suboptimal sensitivity. To overcome this limitation, a number of methodologies based on microfluidics have been developed for sorting circulating tumor cells from peripheral blood of patients with leukemia.In order to develop a more convenient method, we designed an analysis protocol using motion microscopy that amplifies cellular micro motions in a captured video by re-rendering pixels to generate extreme magnified visuals. Intriguingly, no fluctuations around leukemic myeloblasts were observed with a motion microscope at any wavelength of 0–10 Hz. However, use of 0.05% hyaluronic acid, one type of non-newtonian fluid, demonstrated fluctuations around leukemic myeloblasts under conditions of 25 μm/s and 0.5–1.5 Hz with a motion microscope.Thus, the non-invasive detection of leukemic myeloblasts can offer a valuable supplementary diagnostic tool for assessment of drug efficacy for monitoring patients with chronic myeloid leukemia.
A sterile insect technique pilot trial on Captiva Island: defining mosquito population parameters for sterile male releases using mark–release–recapture
Authors: Carvalho Danilo O. , Morreale Rachel , Stenhouse Steven , Hahn Daniel A. , Gomez Maylen , Lloyd Aaron , Hoel David 
Published: November 2022

Abstract
Background
The sterile insect technique (SIT), which involves area-wide inundative releases of sterile insects to suppress the reproduction of a target species, has proven to be an effective pest control method. The technique demands the continuous release of sterilized insects in quantities that ensure a high sterile male:wild male ratio for the suppression of the wild population over succeeding generations.

Methods
For these releases, it is important to determine several ecological and biological population parameters, including the longevity of the released males in the field, the dispersal of the released males and the wild pest population size. The Lee County Mosquito Control District initiated a study in a 47-ha portion of Captiva Island (Florida, USA), an island with a total area of 230 ha, to define biological SIT parameters for Aedes aegypti (L.), an invasive disease-vectoring mosquito known to be difficult to control due to a combination of daytime biting activity, use of cryptic breeding habitats that are difficult to target with conventional night-time ultra-low volume methods, and emerging resistance to commonly used insecticides. Another goal was to assess patterns of dispersal and survival for laboratory-reared sterile Ae. aegypti males released over time in the pilot site. These parameters will be used to evaluate the efficacy of a SIT suppression program for Ae. aegypti on Captiva Island.

Results
Over the course of seven mark-release-recapture studies using single- and multiple-point releases, 190,504 sterile marked males were released, for which the recapture rate was 1.5% over a mean period of 12 days. The mean distance traveled by sterile males of the local strain of Ae. aegypti that has colonized Captiva Island was 201.7 m from the release point, with an observed maximum traveled distance of 404.5 m. The released sterile mosquitoes had a probability of daily survival of 0.67 and an average life expectancy of ~ 2.46 days.

Conclusions
These data together with the population size estimate and sterile:wild ratio provide a solid basis for planning the SIT operational phase which is aimed at mosquito population suppression.

Graphical abstract
Melanoma differentiation-associated gene 5 amyopathic dermatomyositis following an acute Mycoplasma pneumoniae infection: a case report
Authors: Hoey Jessica , Solomon Jenny Lue , Kim Brandon , Carsons Steven , Nusbaum Julie 
Published: November 2022

Abstract
Background
A previously healthy young male of Southeast Asian descent presented with 6 weeks of fevers, cough, mucocutaneous ulcers, arthritis, and myalgias. Initial workup revealed positive Mycoplasma pneumoniae immunoglobulin M, and the patient was treated with antibiotics without relief of symptoms. Rheumatologic workup revealed highly positive melanoma differentiation-associated gene 5 antibody. Viral infections are thought to potentially trigger loss of self tolerance, and prompt the autoimmunity cascade that can result in conditions such as dermatomyositis. To our knowledge, this is the first case report demonstrating a non-viral infection, specifically Mycoplasma pneumoniae, as the inciting infectious trigger for the anti-melanoma differentiation-associated gene 5 dermatomyositis subtype.

Case presentation
A 20-year-old southeast Asian–American male with no significant past medical history presented with symptoms of intermittent fevers, nonproductive cough, dry eyes, oral ulcers, rash, arthritis, and myalgias. The patient was noted to have erythematous papules across the bilateral hands along the lateral digits and palms, as well as synovitis involving the bilateral hands and feet. Immunoglobulin M antibodies were positive for Mycoplasma pneumoniae. The patient was diagnosed with mycoplasma pneumonia. The patient did not respond to a course of antibiotics, leading to rheumatological testing that found highly positive melanoma differentiation-associated gene 5 autoantibody. Muscle enzyme and electromyography testing were normal, indicating clinically amyopathic disease. Methylprednisolone was initiated, with resolution of fevers and improvement of arthritis and myalgias. The cutaneous lesions on the digits and palms improved.

Conclusions
This patient presented with symptoms of fever, cough, oral ulcers, rashes, and arthritis, and blood work demonstrated the presence of immunoglobulin M antibodies to Mycoplasma pneumoniae. Despite antibiotic treatment for the presumed diagnosis of Mycoplasma pneumoniae infection, the patient did not improve, prompting rheumatological workup and revealing melanoma differentiation-associated gene 5 autoantibodies. This case suggests that infections, other than viral, can trigger the autoinflammatory cascade, leading to the development of amyopathic melanoma differentiation-associated gene 5 dermatomyositis.
Decision Analysis for the Influence of Incorporating Waste Materials on Green Concrete Properties
Authors: Bakhoum Emad S. , Mater Yasser M. 
Published: November 2022

AbstractConcrete industry is challenged by sustainability and technical concerns. Sustainability includes minimization of raw material usage, energy consumption, and emission of greenhouse gases, while technical concerns comprise the enhancement of mechanical properties and durability such as compressive strength, resistance to chloride, acids, and elevated temperatures. Therefore, recycling of industrial waste in manufacturing of green concrete has become a robust viable alternative to disposal, due to the limited natural resources and raw materials which contribute to sustainable construction. Consequently, this research aims to develop an approach using a multicriteria decision-making algorithm based on Analytical Hierarchy Process (AHP), to select the most suitable industrial waste to achieve the desired green concrete properties. The research starts by determining the alternatives including 18 industrial wastes, and the criteria including 14 properties of concrete. After that, an experimental database for the influence of the alternatives on the criteria is established based on the literature. Then, an algorithm is developed using a python script to analyze the influence of incorporating each of the industrial waste alternative on both the mechanical and sustainable properties of concrete. Subsequently, the efficiency of the proposed algorithm is validated using three case studies that present different circumstances of concrete specifications. Based on the proposed approach, the decision-maker can assign the appropriate residual waste to be incorporated into the concrete mix according to its application in a user-friendly manner. Such approach can support both sustainable use of waste materials and enhancement of concrete properties.
A glimpse into the monetary supply network of the Tang empire in the seventh century CE: archaeometallurgical study of Kaiyuan Tongbao coins from Lafu Queke cemetery, Xinjiang, Northwest China
Authors: Ma Ding , Wang Yongqiang , Yang Jun , Bi Yanqi , Luo Wugan 
Published: November 2022

Abstract
The supply of coins and their metal materials is important for state governance. Many details about the monetary supply network in ancient China still need to be verified. In this paper, 15 early Tang dynasty Kaiyuan Tongbao 開元通寶 coins excavated from the Lafu Queke 拉甫却克 cemetery, Hami 哈密 city, were studied by combining scientific analyses (PXRF and MC-ICP-MS etc.) with the archaeological context and historical texts. The results show that all of these coins are made of Cu-Sn–Pb ternary alloy. The lead isotopes match with the isotopic signatures of some southwest China lead mines consistent with historical records, 14 coins are located in the range of Southern China geochemical province, and the lead of 5 coins could be considered as highly radiogenic lead (HRL). Combining these results with the archaeological context and historical texts, it can be inferred that the coins minted in southwest China were made from locally exploited raw metal, and supplied to the northwest borderland of the Tang dynasty in the seventh century CE.
Metallothionein synthesis increased by Ninjin-yoei-to, a Kampo medicine protects neuronal death and memory loss after exposure to amyloid β1-42
Authors: Tamano Haruna , Tokoro Haruna , Murakami Daichi , Tsujimoto Rin , Nishijima Yuka , Tsuda Erina , Watanabe Satoshi , Suzuki Miki , Takeda Atsushi 
Published: November 2022

Abstract
Background
It is possible that increased synthesis of metallothioneins (MTs), Zn2+-binding proteins is linked with the protective effect of Ninjin-yoei-to (NYT) on Zn2+ toxicity ferried by amyloid β1-42 (Aβ1-42).

Methods
Judging from the biological half-life (18-20 h) of MTs,
the effective period of newly synthesized MT on capturing Zn2+ is
estimated to be approximately 2 days. In the present paper, a diet containing
3% NYT was administered to mice for 2 days and then Aβ1-42 was injected
into the lateral ventricle of mice.

Results
MT level in the dentate granule cell layer was elevated 2 days after administration of NYT diet, while the administration reduced intracellular Zn2+ level increased 1 h after Aβ1-42 injection, resulting in rescuing neuronal death in the dentate granule cell layer, which was observed 14 days after Aβ1-42 injection. Furthermore, Pre-administration of NYT diet rescued object recognition memory loss via affected perforant pathway long-term potentiation after local injection of Aβ1-42 into the dentate granule cell layer of rats.

Conclusion
The present study indicates that pre-administration of NYT diet for 2 days increases synthesis of MTs, which reduces intracellular Zn2+ toxicity ferried by extracellular Aβ1-42, resulting in protecting neuronal death in the dentate gyrus and memory loss after exposure to Aβ1-42.
The Break-Fast study protocol: a single arm pre-post study to measure the effect of a protein-rich breakfast on autophagic flux in fasting healthy individuals
Authors: Bensalem Julien , Heilbronn Leonie K. , Gore Jemima R. , Hutchison Amy T. , Sargeant Timothy J. , Fourrier Célia 
Published: November 2022

Abstract
Background
Autophagy is a cellular process that cleanses cells and is particularly important during ageing. Autophagy has been extensively studied in vitro and in animal models and is known to be sensitive to nutrition. However, human data are limited because autophagic flux (autophagic degradative activity) has been challenging to measure in humans. This protocol paper describes the Break-Fast study, in which autophagic flux will be measured using a recently developed blood test, before and after ingestion of whey protein. This aims to determine whether an acute nutritional intervention can change autophagy in humans.

Methods
A minimum of forty healthy participants (both male and female) aged 20–50 years, BMI 18.5–29.9 kg/m2 will be recruited into this single arm pre-post study. Participants will visit the clinic after an overnight fast for a first blood collection after which they will consume a whey protein-rich drink. A second blood collection will be performed 60 minutes after consumption of the drink. The primary outcome is the change in autophagic flux at 60 minutes post drink. Secondary outcomes include changes in blood glucose, autophagy-related proteins and mRNA, plasma hormones (e.g. insulin, C-peptide, adiponectin, GLP-1, GIP, ghrelin), cytokines, amino acids and lipids, protein synthesis, and correlation between molecular cell damage and autophagic flux.

Discussion
This study will provide information about whether autophagy responds to nutrients in humans, and if nutritional strategies could be used to treat or prevent autophagy-related diseases such as Alzheimer’s disease or cancer.

Trial registration
Australian New Zealand Clinical Trials Registry (ANZCTR), anzctr.org.au ACTRN12621001029886. Registered on 5 August 2021.
Acrostichium aureum Linn: traditional use, phytochemistry and biological activity
Authors: Akinwumi Kazeem A. , Abam Esther O. , Oloyede Sofiat T. , Adeduro Mary N. , Adeogun Yusuf A. , Uwagboe Juanian E. 
Published: November 2022

Abstract
Background
Acrostichum aureum L is an edible mangrove plant fern that grows mainly in tropical and subtropical regions of the world. This review was conducted to provide in-depth information regarding the traditional uses, phytochemistry and biological activity of A. aureum.

Methods
Scientific literatures were systematically searched using databases including Google scholar, PubMed, Science Direct and ResearchGate for ethnobotany, phytochemistry and pharmacology of the plant. Its potential pharmaceutical and nutritional applications as well as knowledge gap in A. aureum research were also documented.

Results
The outcome revealed that A. aureum is used traditionally across the world to treat several ailments including, non-healing ulcers, boil, wounds, snakebite, bleeding, worm infection, asthma, sore throat, constipation and elephantiasis. Secondary metabolites including, sterols, glycosides, saponins, alkaloids, tannins, flavonoids, phthalates, and terpenoids have been identified in A. aureum. Beneficial phytochemicals including kaempferol, di-(2-methylheptyl) phthalate, β-sitosterol, (2S,3S)-sulfated pterosin C, (+)-pinoresinol-4-O-sulfate, lupeol, α-amyrin and phytol have been detected and/or isolated in the plant. In vitro and in vivo studies also proved that various extracts and phytochemicals in A. aureum have powerful antioxidant, anti-inflammatory, antiulcer, tyrosinase inhibiting, anthelmintic, anti-diarrheal, analgesic, anti-tumor, anti-fertility, anticancer, antibacterial, anti-viral and wound healing properties.

Conclusion
The A.aureum could be harnessed for novel bioactive compounds that can be useful in the treatment of various diseases. Consequently, metabolomic and chemoinformatic analyses could be deployed to fast-track drug discovery and development from the plant. Moreover, safety and activity guided bioassays as well as clinical trials are needed before it could be recommended for clinical use.
LBODP005 Gper Modulates Multiple Functions In Adipose Tissue To Promote An Anti-obese Phenotype
Authors: Sharma Geetanjali , Woods Christine D , Rajkarnikar Resha , Hathaway Helen J , Prossnitz Eric R 
Published: November 2022

Abstract
Obesity and metabolic syndrome are major health issues of modern society and their incidence is increasing at alarming rates worldwide. Obesity and associated chronic diseases exhibit sexual disparity with pre-menopausal females displaying better protection compared to age-matched males. These beneficial effects on metabolism in females are predominantly attributed to the female hormone estrogen, specifically 17β-estradiol (E2). E2 exerts its actions via multiple receptors, nuclear and extra-nuclear estrogen receptor (ER) α and ERβ, and the G protein-coupled estrogen receptor (GPER). GPER is present in diverse cells types and tissues and has been implicated in numerous physiological processes via multiple cellular signaling pathways. We have combined in vivo studies in an ovariectomized (OVX) mouse model along with in vitro assays in 3T3-L1 cells to investigate the role of GPER in modulating adipocyte function. Our studies reveal that loss of GPER increased adiposity in female mice on high fat diet and activation of GPER by its selective agonist G-1 attenuates adiposity in OVX mice. G-1 activated AMPK, increased cellular respiration and expression of mitochondrial and antioxidant genes and reduced lipid deposition in adipocytes. Furthermore, G-1 treatment promoted an anti-inflammatory phenotype in mice. Thus, our findings establish a role of GPER agonist G-1 in exerting anti-lipogenic and anti-inflammatory effects in adipocytes, which may have potential therapeutic implications in treatment of obesity.
Presentation: No date and time listed