bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accurate categorisation of menopausal status for research studies: a step-by-step guide and detailed algorithm considering age, self-reported menopause and factors potentially masking the occurrence of menopause
Authors: Yap Sarsha , Vassallo Amy , Goldsbury David E. , Salagame Usha , Velentzis Louiza , Banks Emily , O’Connell Dianne L. , Canfell Karen , Steinberg Julia 
Published: March 2022

Abstract
Objective
Menopausal status impacts risk for many health outcomes. However, factors including hysterectomy without oophorectomy and Menopausal Hormone Therapy (MHT) can mask menopause, affecting reliability of self-reported menopausal status in surveys. We describe a step-by-step algorithm for classifying menopausal status using: directly self-reported menopausal status; MHT use; hysterectomy; oophorectomy; intervention timing; and attained age. We illustrate this approach using the Australian 45 and Up Study cohort (142,973 women aged ≥ 45 years).

Results
We derived a detailed seven-category menopausal status, able to be further consolidated into four categories (“pre-menopause”/“peri-menopause”/“post-menopause”/“unknown”) accounting for participants’ ages. 48.3% of women had potentially menopause-masking interventions. Overall, 93,107 (65.1%), 9076 (6.4%), 17,930 (12.5%) and 22,860 (16.0%) women had a directly self-reported “post-menopause”, “peri-menopause”, “pre-menopause” and “not sure”/missing status, respectively. 61,464 women with directly self-reported “post-menopause” status were assigned a “natural menopause” detailed derived status (menopause without MHT use/hysterectomy/oophorectomy). By accounting for participants’ ages, 105,817 (74.0%) women were assigned a “post-menopause” consolidated derived status, including 15,009 of 22,860 women with “not sure”/missing directly self-reported status. Conversely, 3178 of women with directly self-reported “post-menopause” status were assigned “unknown” consolidated derived status. This algorithm is likely to improve the accuracy and reliability of studies examining outcomes impacted by menopausal status.
Roles for the methyltransferase SETD8 in DNA damage repair
Authors: Xu Libo , Zhang Ling , Sun Jicheng , Hu Xindan , Kalvakolanu Dhan V. , Ren Hui , Guo Baofeng 
Published: March 2022

AbstractEpigenetic posttranslational modifications are critical for fine-tuning gene expression in various biological processes. SETD8 is so far the only known lysyl methyltransferase in mammalian cells to produce mono-methylation of histone H4 at lysine 20 (H4K20me1), a prerequisite for di- and tri-methylation. Importantly, SETD8 is related to a number of cellular activities, impinging upon tissue development, senescence and tumorigenesis. The double-strand breaks (DSBs) are cytotoxic DNA damages with deleterious consequences, such as genomic instability and cancer origin, if unrepaired. The homology-directed repair and canonical nonhomologous end-joining are two most prominent DSB repair pathways evolved to eliminate such aberrations. Emerging evidence implies that SETD8 and its corresponding H4K20 methylation are relevant to establishment of DSB repair pathway choice. Understanding how SETD8 functions in DSB repair pathway choice will shed light on the molecular basis of SETD8-deficiency related disorders and will be valuable for the development of new treatments. In this review, we discuss the progress made to date in roles for the lysine mono-methyltransferase SETD8 in DNA damage repair and its therapeutic relevance, in particular illuminating its involvement in establishment of DSB repair pathway choice, which is crucial for the timely elimination of DSBs.
DWI-based radiomic signature: potential role for individualized adjuvant chemotherapy in intrahepatic cholangiocarcinoma after partial hepatectomy
Authors: Yang Yang , Zou Xianlun , Zhou Wei , Yuan Guanjie , Hu Daoyu , Shen Yaqi , Xie Qingguo , Zhang Qingpeng , Kuang Dong , Hu Xuemei , Li Zhen 
Published: March 2022

Abstract
Objectives
To develop a diffusion-weighted imaging (DWI) based radiomic signature for predicting early recurrence (ER) (i.e., recurrence within 1 year after surgery), and to explore the potential value for individualized adjuvant chemotherapy.

Methods
A total of 124 patients with intrahepatic cholangiocarcinoma (ICC) were randomly divided into the training (n = 87) and the validation set (n = 37). Radiomic signature was built using radiomic features extracted from DWI with random forest. An integrated radiomic nomogram was constructed with multivariate logistic regression analysis to demonstrate the incremental value of the radiomic signature beyond clinicopathological-radiographic factors. A clinicopathological-radiographic (CPR) model was constructed as a reference.

Results
The radiomic signature showed a comparable discrimination performance for predicting ER to CPR model in the validation set (AUC, 0.753 vs. 0.621, p = 0.274). Integrating the radiomic signature with clinicopathological-radiographic factors further improved prediction performance compared with CPR model, with an AUC of 0.821 (95%CI 0.684–0.959) in the validation set (p = 0.01). The radiomic signature succeeded to stratify patients into distinct survival outcomes according to their risk index of ER, and remained an independent prognostic factor in multivariable analysis (disease-free survival (DFS), p < 0.0001; overall survival (OS), p = 0.029). Furthermore, adjuvant chemotherapy improved prognosis in high-risk patients defined by the radiomic signature (DFS, p = 0.029; OS, p = 0.088) and defined by the nomogram (DFS, p = 0.031; OS, p = 0.023), whereas poor chemotherapy efficacy was detected in low-risk patients.

Conclusions
The preoperative DWI-based radiomic signature could improve prognostic prediction and help to identify ICC patients who may benefit from postoperative adjuvant chemotherapy.
A novel missense mutation in complement factor I predisposes patients to atypical hemolytic uremic syndrome: a case report
Authors: Wei Xin , Li Juan , Zhan Xiaojiang , Tu Luxia , Huang Haowen , Wang Ying 
Published: March 2022

Abstract
Background
Atypical hemolytic uremic syndrome, also called the nondiarrheal form of hemolytic uremic syndrome, is a rare disease characterized by the triad of thrombocytopenia, Coomb’s test-negative microangiopathic hemolytic anemia, and acute renal failure. Approximately 60% of cases of atypical hemolytic uremic syndrome are associated with deficiencies of the complement regulatory protein, including mutations in complement factor H, complement factor I, or the membrane co-factor protein.

Case presentation
We report the case of a 26-year-old Asian man who presented with pulmonary infection, elevated blood pressure, microangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure. Renal biopsy revealed diffuse capillary fibrin deposition, endothelial swelling, and arteriole narrowing like “onion skinning” consistent with thrombotic microangiopathy. Bidirectional sequencing of CFH, CFHR5, CFHR1, CFI, DGKE, CFB, and MCP confirmed that the patient was heterozygous for a novel missense mutation, p.Cys67Phe, in CFI. This patient had rapid evolution to end-stage renal disease and needed renal replacement therapy. Plasma exchange seemed inefficacious in this patient.

Conclusions
This report confirms the importance of screening patients with atypical hemolytic uremic syndrome for mutations in genes involved in complement system to clarify the diagnosis and demonstrates the challenges in the management of these patients.
Scedosporium apiospermium keratitis: a case report
Authors: Karaca Umut 
Published: March 2022

Abstract
Background
Scedosporium apiospermum, an opportunistic and filamentous fungus, is a rarely seen ocular entity that is difficult to identify and heal. We report a challenging case of S. apiospermium keratitis and discuss the treatment modalities in light of previous studies.

Case presentation
A 30-year-old Turkish farmer with a history of contact lens misuse presented to our clinic with a painful corneal abscess and severe vision loss in his left eye. S. apiospermum was identified by spectrophotometric analysis. The patient was successfully treated with therapeutic penetrating keratoplasty, but was resistant to fluconazole and amphotericin B and susceptible but unresponsive to voriconazole.

Conclusion
S. apiospermum keratitis should be considered in the differential diagnosis of immunocompromised and immunocompetent patients with history of ocular trauma and contact lens use, especially those who do not respond to treatment.
Intensive care unit capacity and mortality in older adults: a three nations retrospective observational cohort study
Authors: Abuhasira Ran , Anstey Matthew , Novack Victor , Bose Somnath , Talmor Daniel , Fuchs Lior 
Published: March 2022

Abstract
Background
Intensive care unit (ICU) admissions among older adults are expected to increase, while the benefit remains uncertain. The availability of ICU beds varies between hospitals and between countries and is an important factor in the decision to admit older adults in the ICU. We aimed to assess if a non-restrictive approach to ICU older adults admission is associated with a corresponding change in survival.

Methods
Retrospective cohort study that included patients ≥ 80 years who were admitted to each of the three participating hospitals in Australia, Israel, and the United States (USA), between the years 2006–2015, each with distinct ICU capacities and admission criteria. The primary outcomes were in-hospital mortality and all-cause mortality at 6, 12, 18, and 24 months following index hospitalization.

Results
The cohort included 62,866 patients with a mean age of 85.9 ± 4.6 years and 58.8% were women. The ICU admission rates were 22.5%, 2.6% and 2.3% in USA, Australia, and Israel, respectively. We constructed a model for ICU admissions based on the USA cohort (highest availability of ICU beds) and then calculated the expected probabilities for the Israeli and Australian cohorts. For the patients in the highest quintile of the admission model, actual ICU admission rates were 67.6% in USA, 22.1% in Australia and 6.0% in Israel. Of these, in-hospital death rates were 52.3% in Israel, 29.8% in Australia, and 22.1% in USA. Two years after hospital discharge, the survival rates in the USA and Australia were 53%, while in Israel 48%.

Conclusion
ICU admission of adults ≥ 80 years is associated with increased in-hospital survival compared to ward admission, but survival rates 2 years later are similar.
Intracranial otogenic complications in adults: New factors that influenced its onset, frequency and nature
Authors: Wierzbicka Małgorzata , Staśkiewicz Michalina , Rosiak Oskar , Karmelita-Katulska Katarzyna , Szyfter Witold , Gawęcki Wojciech 
Published: March 2022

Background To compare the clinical features of two time cohorts of patients: “pre-COVID-19” and “COVID-19”—admitted as emergency with intracranial otogenic complications, with special regard to sigmoid sinus thrombosis (CVST). Methods Retrospective analysis of patients documentation concerning urgent procedures of intracranial otogenic complications at tertiary-referral otolaryngology department. Analysed database—pre-COVID-19 cohort (January–February 2019/2020): 1434 otological outpatient visits, 509 planned otosurgeries and 17 urgent otological procedures; COVID-19 cohort (March–April 2020/2021): 1150, 566 and 20 respectively. Overall intracranial complications: 5 and 9 respectively. Analysed outcome measures: incidence proportion of otogenic intracranial complications in relation to planned and urgent otosurgical procedures; incidence proportion of intracranial complications in relation to the total number of emergency and planned outpatient consultations and the total number of planned surgical procedures. Results There were 14 intracranial complications, 5 in the pre-COVID and 9 in the COVID cohort, including 1 and 5 sigmoid sinus thrombosis, respectively. Out of them, 3 and 5 patients reported a prior history of chronic otitis media, respectively. In COVID period, CVST was more prevalent, with 2 cases (22.2%) presenting solitary CVST, and 3 cases (33.3%) CVST and a simultaneous brain abscess or meningitis. CVST was much more frequent in the COVID period (p < 0.01). Conclusions Despite the published data which suggest that CVST is a rare event associated with COVID-19 infection, based on our experience, CVST can be expected as a frequent component of intracranial otogenic complications during COVID-19 pandemic time. Trial registration This research study was conducted retrospectively from data obtained for clinical purposes. We consulted extensively with the Bioethics Committee at Poznan University of Medical Sciences who determined that our study did not need ethical approval. An official waiver of ethical approval was granted from the Bioethics Committee at Poznan University of Medical Sciences. Graphical Abstract
Rapid prototyping for series of tasks in atypical environment: robotic system with reliable program-based and flexible learning-based approaches
Authors: Ito Hiroshi , Nakamura Satoshi 
Published: March 2022

AbstractWe propose a novel robotic system that combines both a reliable programming-based approach and a highly generalizable learning-based approach. How to design and implement a series of tasks in an atypical environment is a challenging issue. If all tasks are implemented using a programming-based approach, the development costs will be huge. However, if a learning-based approach is used, reliability is an issue. In this paper, we propose novel design guidelines that focus on the respective advantages of programming-based and learning-based approaches and select them so that they complement each other. We use a program-based approach for motions that is rough behavior and a learning-based approach for motion that is required complex interaction between robot and object of robot tasks and are difficult to achieve with a program. Our learning approach can easily and rapidly accomplish a series of tasks consisting of various motions because it does not require a computational model of an object to be designed in advance. We demonstrate a series of tasks in which randomly arranged parts are assembled using an actual robot.
Internal Training Load Perceived by Athletes and Planned by Coaches: A Systematic Review and Meta-Analysis
Authors: Inoue Allan , dos Santos Bunn Priscila , do Carmo Everton Crivoi , Lattari Eduardo , da Silva Elirez Bezerra 
Published: March 2022

Abstract
Background
Rating of perceived exertion (RPE) and session RPE (sRPE) has been widely used to verify the internal load in athletes. Understanding the agreement between the training load prescribed by coaches and that perceived by athletes is a topic of great interest in sport science.

Objective
This systematic review and meta-analysis aimed to investigate differences between the training/competition load perceived by athletes and prescribed/intended/observed by coaches.

Methods
A literature search (September 2020 and updated in November 2021) was conducted using PubMed, Web of Science, Embase, and SPORTDiscus databases. The protocol was registered in the Open Science Framework (osf.io/wna4x). Studies should include athletes and coaches of any sex, age, or level of experience. The studies should present outcomes related to the RPE or sRPE for any scale considering overall training/competition sessions (physical, strength, tactical, technical, games) and/or classified into three effort categories: easy, moderate, and hard.

Results
Twenty-seven studies were included in the meta-analysis. No difference was found between coaches and athletes for overall RPE (SMD = 0.19, P = 0.10) and overall sRPE (SMD = 0.05, P = 0.75). There was a difference for easy RPE (SMD = − 0.44, small effect size, P = 0.04) and easy sRPE (SMD = − 0.54, moderate effect size, P = 0.04). No differences were found for moderate RPE (SMD = 0.05, P = 0.74) and hard RPE (SMD = 0.41, P = 0.18). No difference was found for moderate (SMD = -0.15, P = 0.56) and hard (SMD = 0.20, P = 0.43) sRPE.

Conclusion
There is an agreement between coaches and athletes about overall RPE and sRPE, and RPE and sRPE into two effort categories (moderate and hard). However, there were disagreements in RPE and sRPE for easy effort category. Thus, despite a small disagreement, the use of these tools seems to be adequate for training monitoring.
Rapid detection of single nucleotide polymorphisms using the MinION nanopore sequencer: a feasibility study for perioperative precision medicine
Authors: Tabata Yoshiteru , Matsuo Yoshiyuki , Fujii Yosuke , Ohta Atsufumi , Hirota Kiichi 
Published: March 2022

Abstract
Introduction
Precision medicine is a phrase used to describe personalized medical care tailored to specific patients based on their clinical presentation and genetic makeup. However, despite the fact that several single nucleotide polymorphisms (SNPs) have been reported to be associated with increased susceptibility to particular anesthetic agents and the occurrence of perioperative complications, genomic profiling and thus precision medicine has not been widely applied in perioperative management.

Methods
We validated six SNP loci known to affect perioperative outcomes in Japanese patients using genomic DNA from saliva specimens and nanopore sequencing of each SNP loci to facilitate allele frequency calculations and then compared the nanopore results to those produced using the conventional dideoxy sequencing method.

Results
Nanopore sequencing reads clustered into the expected genotypes in both homozygous and heterozygous cases. In addition, the nanopore sequencing results were consistent with those obtained using conventional dideoxy sequencing and the workflow provided reliable allele frequency estimation, with a total analysis time of less than 4 h.

Conclusion
Thus, our results suggest that nanopore sequencing is a promising and versatile tool for SNP genotyping, allowing for rapid and feasible risk prediction of perioperative outcomes.
LPAR2 correlated with different prognosis and immune cell infiltration in head and neck squamous cell carcinoma and kidney renal clear cell carcinoma
Authors: Sun Kai , Chen Ri-xin , Li Jing-zhang , Luo Zhan-xiong 
Published: March 2022

Abstract
Background
Lysophosphatidic acid (LPA) and its receptors play a key role in regulating cancer progression. Upregulation of LPA receptor 2 (LPAR2) plays a role in carcinogenesis; however, the exact role of LPAR2 in tumors remains elusive. This study aims to explore the correlation between LPAR2 expression with tumor prognosis and immune infiltration in pan-cancers.

Materials and methods
The expression of LPAR2 in pan-cancers was analyzed using the Online Cancer Microarray Database (Oncomine), Tumor Immune Estimation Resource (TIMER), and UALCAN databases. The effects of LPAR2 on the clinical prognosis in pan-cancer were examined using the Kaplan–Meier plotter (KM plotter) as well as Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN, and Human Protein Atlas (HPA) databases. Moreover, the R software program was applied for validation of expression and prognostic value of LPAR2 in tumor patients in the Cancer Genome Atlas (TCGA) dataset and the Gene Expression Omnibus (GEO) database. The relationship between the expression level of LPAR2 and the clinical and molecular criteria of head and neck squamous cell carcinoma (HNSC) and kidney renal clear cell carcinoma (KIRC) was analyzed using UALCAN, whereas the relationship between LPAR2 expression and prognosis in patients with HNSC and KIRC with different clinical characteristics was examined using the KM plotter. Furthermore, the correlation between LPAR2 expression and tumor immune infiltration was examined using TIMER. The correlation between LPAR2 expression and gene markers of tumor immune infiltrates was analyzed using TIMER and GEPIA. In addition, the cBioPortal for Cancer Genomics was used to calculate the mutations, methylations, and altered neighbor genes of LPAR2.

Results
The expression of LPAR2 was significantly correlated with the outcome of multiple types of cancer, especially HNSC and KIRC. Furthermore, high expression of LPAR2 was significantly associated with various immune markers in the immune cell subsets of HNSC and KIRC.

Conclusions
High expression of LPAR2 plays significantly different prognostic roles in HNSC and KIRC possibly owing to its association with different immune markers. LPAR2 is correlated with tumor immune cell infiltration and is a valuable prognostic biomarker for HNSC and KIRC. However, further experiments are required to validate these findings.
Interaction of prior category knowledge and novel statistical patterns during visual search for real-world objects
Authors: Moon Austin , Zhao Jiaying , Peters Megan A. K. , Wu Rachel 
Published: March 2022

AbstractTwo aspects of real-world visual search are typically studied in parallel: category knowledge (e.g., searching for food) and visual patterns (e.g., predicting an upcoming street sign from prior street signs). Previous visual search studies have shown that prior category knowledge hinders search when targets and distractors are from the same category. Other studies have shown that task-irrelevant patterns of non-target objects can enhance search when targets appear in locations that previously contained these irrelevant patterns. Combining EEG (N2pc ERP component, a neural marker of target selection) and behavioral measures, the present study investigated how search efficiency is simultaneously affected by prior knowledge of real-world objects (food and toys) and irrelevant visual patterns (sequences of runic symbols) within the same paradigm. We did not observe behavioral differences between locating items in patterned versus random locations. However, the N2pc components emerged sooner when search items appeared in the patterned location, compared to the random location, with a stronger effect when search items were targets, as opposed to non-targets categorically related to the target. A multivariate pattern analysis revealed that neural responses during search trials in the same time window reflected where the visual patterns appeared. Our finding contributes to our understanding of how knowledge acquired prior to the search task (e.g., category knowledge) interacts with new content within the search task.