bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changing in gamma aminobutyric acid and phytic acid during the development of yogurt- like product from germinated hyacinth bean
Authors: Nguyen Duc Doan , Vu Huong Quynh 
Published: May 2022

Abstract
Hyacinth bean was used for developing a functional yogurt-like product with a high level of gamma aminobutyric acid (GABA). Germinated hyacinth bean extract was fermented with three different yogurt cultures to pH 4.5 and then stored at 4–6°C for 21 days. The variation in GABA and phytic acid levels during cold storage was evaluated. The type of culture and storage time significantly influenced GABA and phytic acid levels. Lactococcus lactis ssp. cremoris, Lc. lactis ssp. lactis and Streptococcus thermophilus produced a yogurt-like product with the highest level of GABA. In addition, the level of GABA and phytic acid significantly decreased during cold storage.
Novel Technique for Intractable Hiccups with Ultrasound-Guided Percutaneous Laser Cervical Discectomy: Case Report
Authors: Ma Jiahui , Jin Yangqing , Ni Yuncheng , Hu Rong , Han Rui , Huang Dong , Zhou Haocheng 
Published: May 2022

Abstract
Study design: Case report and technical note.Objective: The aim of this study is to report our novel ultrasound-guided technique of percutaneous laser cervical discectomy in one successful case of intractable hiccups.Summary of background data: Intractable hiccups serve no physiological purpose, but significantly reduce the quality of life. There are a variety of medications suggested for the treatment of persistent or intractable hiccups. However, it remains a challenge for the management of intractable hiccups.Methods: A 41-year-old male, who suffered intractable hiccups over 11 years, came to our pain department in December 2020. Neither oral medication nor phrenic nerve block achieved satisfactory relief of hiccups. Magnetic resonance imaging and computed tomography scan revealed cervical disc herniation of C4/5 and C5/6. Following selective cervical nerve root block, complete but short-term control of symptoms lasted less than 48 hours. Percutaneous laser cervical discectomy was then performed under ultrasound guidance. Results: The pathological hiccup reflex was terminated immediately after the minimally-invasive procedure, and the enduring therapeutic effect was reported up to the 14-month follow-up. We did not observe any obvious complications in this case.Conclusions: Ultrasound-guided percutaneous laser cervical discectomy may be an alternative and safe option for those with intractable hiccups caused by cervical discogenic etiology.
Disrupted effective connectivity of the periaqueductal gray in migraine without aura patients
Authors: Liu Xiaozheng , Cheng Jinming , Cao Yungang , Ke Yang , Li Yan , Zhang Xi , Wang Zhihong , Liu Kun , Dong Lipeng 
Published: May 2022

Abstract
Background: The periaqueductal gray (PAG) is a key region in the descending pain modulatory system. We applied a Granger causality analysis (GCA) based approach to examine resting-state effective connectivity (RSEC) of the bilateral PAG regions in migraine patients without aura (MwoA).Methods: Resting-state fMRI data were obtained from 28 MwoA patients and 17 well-matched healthy controls (HCs). The RSEC of the bilateral PAG was characterized using a voxel-wised GCA method. The resulting RSEC measurements were assessed for correlations with other clinical features.Results: Compared with the HCs, MwoA patients showed increased EC from left PAG to left anterior cingulate gyrus and right postcentral gyrus. Meanwhile, MwoA patients also showed increased EC from right PAG to left precentral gyrus and increased RSEC from left caudate and right middle occipital gyrus to right PAG.Conclusions: Disturbances of RSEC between PAG and limbic system, primary sensorimotor cortex, and visual cortex may play a key role in neuropathological features, perception, and affection of MwoA. The current study provides further insights into the complex scenario of MwoA mechanisms.
The role of postoperative radiotherapy in patients with vulvar squamous carcinoma: findings based on the SEER database
Authors: Wang Li , Wang Tao , Sun Jing 
Published: May 2022

Abstract
Introduction: The role of postoperative radiotherapy in treating squamous cell carcinoma of the vulva remains controversial. This study evaluated the effect of radiotherapy on the survival of patients with postoperative squamous cell carcinoma of the vulva.Methods： Clinical and prognostic information on patients diagnosed with vulvar squamous cell carcinoma from 2010 to 2015 was collected from the Surveillance, Epidemiology, and Prognosis (SEER) database. A propensity score matching (PSM) approach was used to balance the differences in clinicopathological characteristics between groups. The impact of postoperative radiotherapy on overall survival (OS) and disease-specific survival (DSS) was assessed.Results： The study included 3629 patients with squamous cell carcinoma of the vulva, of whom 767 (21.1%) underwent postoperative radiotherapy. After propensity score matching, multivariate analysis showed that Age, M stage, tumor size, and lymph node surgery were independent prognostic factors affecting patient survival. Postoperative radiotherapy improved patients' overall survival and disease-specific survival. Further subgroup survival analysis showed that overall survival was significantly improved among patients who received radiotherapy in patients with Grade III, IV, AJCC stage III, N1, lymph node metastasis, large tumor diameter, and those who received chemotherapy.Conclusion: Postoperative radiotherapy may provide a survival benefit for patients with squamous cell carcinoma of the vulva, especially for AJCC stage III, lymph node metastases, large tumor diameter, and those receiving postoperative chemotherapy.
A Novel approach towards Neuro Acoustic Loops and their intervention in various Neuro Psychiatric Disorders
Authors: Aasim Shahzad , Ahmad Suhail , Butt Muheet , Wani Hilal , Fatima Rifat , Ganie Abdul , Dar Nizam Ud Din 
Published: May 2022

Abstract
Acoustic signals are not transmitted to the brain wholly by contrivances of the ear but influences us through the mediums of skin, bones, and viscera. The ear is awfully subtle to vibrations in the air and conveys the tessellations of these vibrations in a form that the brain distinguishes as sound and speech. The medical devices are being developed that consume high-intensity focused acoustic signals as a non-invasive method for diagnostics and treatment of various psychiatric ailments. The major benefit of these sound or music & acoustic signals that offers the technique so readily to use in non-invasive therapy is its ability to breach deep into the human body and supply to a specific site thermal or mechanical energy with sub-millimetre accuracy. In this paper an attempt is made to study the intervention of these sound frequencies in general and their effects on neuro-psychiatric mechanisms and allied phenomenon. The quantification of specific frequencies (Acoustic loops) which will ameliorate the various disorders after their identification can also be correlated with various biochemical parameters that will be analysed with regards to the changes effected by these interventions. The proposed research work might provide a novel approach of treatment for neuro psychiatric disorders using the above stated measures using acoustic loops intervention that uses specific spectral frequencies to trigger effective and discernible changes in the subjects/patients suffering from neuropsychiatric ailments etc. The work intends to provide the effect of the above mentioned on 50 subjects which are taken in isolation wherein the results are quite demanding.
The hsa_circ_0024183 may affects glioblastoma progression by interacting with the protooncogene SALL4
Authors: Lei Zhang , Qi Wang , Junle Zhu , Jingliang Ye , Fenglin Zhang , Feng Chen , Jun Qian , Chun Luo , Huairui Chen , Qing Zhang 
Published: May 2022

Abstract
Purpose: The pathogenesis of glioma is not clear, SALL4 is a protooncogene and has been shown to promote the growth of glioma cells. CircRNAs have been shown to play a role in the development of tumors, but the relationship between SALL4 and circRNAs has not been well characterized. The aim of this study is to establish the association between SALL4 and circRNAs.Methods: The circRNAs and miRNAs related to SALL4 screened before were detected in glioma and normal brain tissue samples, then hsa_circ_0024183 was selected to determine its effect on glioma progression in vivo and vitro. The interaction between hsa_circ_0024183, miR-485-5p and SALL4 was clarified by double luciferase assay and RNA immunoprecipitation analysis.Results: The expression of hsa_circ_0024183 was significantly higher in glioma patients and human glioblastoma cell lines than in normal brain(P<0.05). GBM patients with higher hsa_circ_0024183 expression had worse outcome. The reduction of hsa_circ_0024183 by siRNA was found to decrease the proliferation, migration, and invasion abilities of GBM cells and block their cell cycles at the S phase. Furthermore, a decrease of hsa_circ_0024183 expression in GBM cells was found to inhibit their tumorigenicity in nude mice. Mechanistic studies revealed that hsa_circ_0024183 can regulate SALL4 expression by sponging miR-485-5p. And hsa_circ_0024183 RNA could directly bind to SALL4 protein.Conclusion: Interference with hsa_circ_0024183 can reduce the expression of SALL4 and the binding of hsa_circ_0024183 to SALL4 protein. It may affect the progression of glioma through the PTEN/PI3K/Akt pathway. This discovery provides an opportunity to develop potential targeted therapy against glioma.
The economic cost of bovine trypanosomosis in pastoral and ago pastoral communities in Buliisa district, Uganda
Authors: Kizza Daniel , Ocaido Michael , Mugisha Anthony , Azuba Rose , Nalule Sarah , Onyuth Howard , Musinguzi Simon Peter , Nalubwama Sylvia , Waiswa Charles 
Published: May 2022

Abstract
Background - Animal diseases that are endemic like tsetse transmitted trypanosomosis cause the continuous expenditure of financial resources of livestock farmers and loss of productivity of livestock. Estimating the cost of controlling animal trypanosomosis can provide evidence for priority setting and targeting cost-effective control strategies.Methodology A cross-sectional survey to estimate the economic cost of bovine trypanosomosis was conducted in cattle-keeping communities living around Murchision falls National Park, in Buliisa district Uganda. Data was collected on herd structure, the cost of treatment and control, prevalence of morbidity and mortality rates due to trypanosomosis, and salvage sales losses in cattle herds in the last year.Results- In this study, 55.4% (n= 87) of the households reported their cattle had been affected by trypanosomosis during the previous last year. There was a high economic cost of trypanosomosis (USD 653) per household in cattle-keeping communities in Buliisa district of which 83% and 9% were due to mortality and milk loss respectively/ High mortality loss was due to low investment in treatment. The study showed that prophylactic treatment 3 times a year of the whole herd of cattle using Samorin ® (Isometamidium chloride) at a cost of USD 110 could drastically reduce cattle mortality loss due to trypanosomosis due to trypanosomosis with a return on investment of USD 540 annually per herd. This could be coupled with strategic restricted insecticide spraying of cattle with deltamethrin products.Conclusions. The results show a high economic cost of trypanosomosis in cattle-keeping communities in Buliisa district, with cattle mortality contributing the largest proportion of the economic cost. The high mortality loss was due to low investment in treatment of sick cattle
Amine Bendahmane1* and Redouane Tlemsani1
Authors: Bendahmane Amine , Tlemsani Redouane 
Published: May 2022

Abstract
Butterfly Optimization Algorithm (BOA) is a recent metaheuristic that has been used in several optimization problems. In this paper, we adapt this metaheuristic to robotics for solving the Unknown Area Exploration problem with energy constraints in both single and multi-robot scenarios. We conducted several experiments to validate the approach and compare its performance to well-known metaheuristics used in the literature using 5 different comparison criteria. We also proposed a new version of the algorithm (xBOA) based on the crossover operator. We compared its results to the original BOA and 3 other variants recently introduced in the literature. Although BOA and xBOA are not optimal in all evaluation criteria, we found that BOA can be a good alternative to many metaheuristics in terms of the exploration time, while xBOA is more robust to local optima; has better fitness convergence; and achieves better exploration rates than the original BOA and its other variants.
Scanning Electron Microscopy and Metabolite Measurement Revealed the Stress Mechanism of PS-COOH Microplastics on Rhodotorula mucilaginosa AN5
Authors: Ma Jiahao , Meng Xiangfei , Li Zixin , Li Lexian , Xu Jiwen , Kan Guangfeng 
Published: May 2022

Abstract
Microplastics in the marine environment have been paid more and more attention by researchers, and the impact of these substances on marine microorganisms can not be ignored. This study aims to pay attention to the effect and mechanism of microplastics (80 nm PS-COOH) on Antarctic marine yeast, Rhodotorula mucilaginosa AN5. In our work, Scanning Electron Microscopy (SEM) was used to observe the morphology of yeast cells under microplastic stress, and metabolite analysis was used to explore the possible mechanism of yeast cell damage. The results showed that: (1) a certain concentration of PS-COOH could inhibit 40% growth of yeast cells and destroy the cell morphology. (2) Physiological and biochemical changes showed that under PS-COOH stress, the level of reactive oxygen species (ROS), malondialdehyde (MDA) content and the activities of antioxidant enzymes such as catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) of R. mucilaginosa AN5 increased from 34–66%. It can be guessed that yeast can eliminate excess ROS in cells by the activity of oxidative kinases increased and maintain the balance of reactive oxygen species in cells.
“It Gives Me, as her Caregiver, a Sense of Security.” Young People with Intellectual Disability and their Experiences with Sexuality, Menstruation, Gynecological Treatment and Contraception: A Follow-up Analysis of Parents’ and Caregivers’ Perspectives
Authors: Retznik Laura , Wienholz Sabine , Höltermann Annelen , Conrad Ines , Riedel-Heller Steffi Gerlinde 
Published: May 2022

Abstract
Background
Research shows that caregivers support but also impede people with intellectual disabilities from developing intimate relationships. People with intellectual disability experience coital sex later and less often compared to adolescents without disabilities. Caregivers often request hormonal contraception from gynecologists on their behalf. Their main motives are pregnancy avoidance (either as a result of consensual sex or sexual abuse) and menstrual suppression.
Method
The sample included 42 primary caregivers of young people with intellectual disability aged 14 to 25. Interviews were evaluated with qualitative content analysis.
Results
Nine of the main caregivers assumed that sexual intercourse had taken place. Half of the carers evaluated the importance of sexuality for the young people with intellectual disability as high. The main reason for seeing a gynecologist was the prescription of hormonal contraceptives; the outcome of which was arbitrary and dependent on the individual gynecologist. The majority of young women took general hormonal contraceptives as a preventive measure or due to heavy bleeding, regardless of sexual activity. One-fifth took hormone depots as a form of long-term contraception.
Discussion
A trusting relationship with the gynecologist favored the compliance and well-being of the young women. The administration of hormonal contraceptives seems to be controlled by third parties. Caregivers used hormonal contraceptives to simplify the regulation of menstruation and felt relieved by it. Carers view the sexual activities of the young women more critically because they fear a pregnancy and the responsibility for a potential grandchild. The use of hormonal contraceptives made carers clearly more tolerant of sexual activity.
Modeling of the combined dynamics of leptospirosis transmission and seroconversion in herds
Authors: Chadsuthi Sudarat , Chalvet-Monfray Karine , Kodjo Angeli , Wiratsudakul Anuwat , Bicout Dominique J. 
Published: May 2022

Abstract
Leptospirosis is a zoonotic disease-causing illness in both humans and animals resulting in related economic impacts due to production loss as well as prevention and control efforts. Several mathematical models have been proposed to study the dynamics of infection but none of them has so far taken into account the dynamics of seroconversion. In this study, we have developed a general framework, based on the kinetic model for animal leptospirosis, that combines both the infection and the antibody dynamics to simultaneously follows both infection and seroconversion status of leptospirosis in a herd population. It is a stochastic compartmental model (for transition rates) with time delay (for seroconversion) which describes the progression of infection by a SEIR (susceptible, exposed, infected and removed) approach and seroconversion by four-state antibody kinetics (antibody negative and three antibody positive states of different antibody levels). The model shows that it is possible to assess and follow both infection and seroconversion status through the prism of diagnostic testing. Such an approach could prove useful to assist the competent authorities in their analyzes of epidemic situations and in the implementation of strategies for controlling and managing the associated risks.
Native T1 and T2 Reference Values for Maltese Healthy Cohort
Authors: Yamagata Kentaro , Yamagata Lara Marie , Abela Mark , Mifsud Claude Portanier , Micallef Lee Ann , Reichmuth Luise , Borg Alexander 
Published: May 2022

Abstract
BackgroundCardiac Magnetic Resonance (CMR) is increasingly being used for diagnosing various cardiac conditions. Parametric mapping enables quantitative myocardial characterization by directly measuring myocardial T1 and T2 values. However, reference values of parametric mapping are not standardized across different vendors and scanners, causing drawbacks for clinical implementation of this technique across different sites. We assessed the reference ranges of native T1 and T2 values in a healthy Maltese cohort to establish a local parametric mapping service. Methods and ResultsHealthy subjects (n=51; mean age 36.0 [range 19-59] years) with normal cardiac function on CMR were recruited. Subjects underwent uniform parametric mapping pulse sequences (MOLLI 5(3)3 for native T1 mapping, and gradient echo single shot FLASH readout for T2 mapping) on a 3T Siemens MAGNETOM Vida scanner. Native T1 and T2 values were measured by placing a region of interest within the interventricular septum at midventricular level. Intra- and inter-observer variability were assessed using Bland-Altman plots. Normal distribution of data was assessed using Kolmogorov-Smirnov test (p-value of 0.773 for native T1, p-value of 0.125 for T2). Mean ±1.96 SD was used as a reference range. Mean native T1 and T2 values were 1200.1 ±30.7 ms and 39.5 ±1.8 ms, respectively. All parameters had excellent day-to-day intra-observer reproducibility and inter-observer reproducibility. ConclusionFor the first time in Malta, we established the native T1 and T2 parametric mapping reference values for healthy Caucasian Maltese individuals. This will assist cardiologists to establish diagnosis, disease progression, and response to treatment of various myocardial diseases locally.
Factors secreted by monosodium urate crystal-stimulated macrophages promote a proinflammatory state in osteoblasts: a potential indirect mechanism of bone erosion in gout
Authors: Naot Dorit , Pool Bregina , Chhana Ashika , Gao Ryan , Munro Jacob T , Cornish Jillian , Dalbeth Nicola 
Published: May 2022

Abstract
Background: Tophi are lesions commonly present at sites of bone erosion in gout-affected joints. The tophus comprises a core of monosodium urate (MSU) crystals surrounded by soft tissue that contains macrophages and other immune cells. Previous studies found that MSU crystals directly reduce osteoblast viability and function. The aim of the current study was to determine the indirect, macrophage-mediated effects of MSU crystals on osteoblasts. Methods: Conditioned medium from the RAW264.7 mouse macrophage cell line cultured with MSU crystals was added to the MC3T3-E1 mouse osteoblastic cell line. Conditioned medium from the THP-1 human monocytic cell line cultured with MSU crystals was added to primary human osteoblasts (HOBs). Matrix mineralization was assessed by von Kossa staining. Gene expression was determined by real-time PCR, and concentrations of secreted factors were determined by enzyme-linked immunosorbent assay. Results: In MC3T3-E1 cells cultured for 13 days in osteogenic medium, the expression of the osteoblast marker genes Col1a1, Runx2, Sp7, Bglap, Ibsp, and Dmp1 was inhibited by conditioned medium from MSU crystal-stimulated RAW264.7 macrophages. Mineral staining of MC3T3-E1 cultures on day 21 confirmed the inhibition of osteoblast differentiation. In HOB cultures, the effect of 20 h incubation with conditioned medium from MSU crystal-stimulated THP-1 monocytes on osteoblast gene expression was less consistent. Expression of the genes encoding cyclooxygenase-2 and IL-6 and secretion of the proinflammatory mediators PGE2 and IL-6 were induced in MC3T3-E1 and HOBs incubated with conditioned medium from MSU crystal-stimulated macrophages/ monocytes. However, inhibition of cyclooxygenase-2 activity and PGE2 secretion from HOBs indicated that this pathway does not play a major role in mediating the indirect effects of MSU crystals in HOBs. Conclusions: Factors secreted from macrophages stimulated by MSU crystals attenuate osteoblast differentiation and induce the expression and secretion of proinflammatory mediators from osteoblasts. We suggest that bone erosion in joints affected by gout results from a combination of direct and indirect effects of MSU crystals.
Regularity of solutions to nonlinear elliptic equations in generalized Morrey spaces
Authors: Guliyev V.S. , Gadjiev T.S. , Serbetci A. , Rustamov Y.I. 
Published: May 2022

Abstract
In this paper, we prove the generalized Morrey estimates for the gradient of weak solutions to a class of nonlinear elliptic equations in a very general irregular domain. The nonlinearity ${\bf a}(x,\xi)$ is assumed to be measurable in $x$ for almost every $\xi$ and belongs to the small $BMO$ class.