bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of physician-patient relationship perceived risk measurement of physician: questionnaire development and reliability testing
Authors: Gao Yufei , Liu Tong , Li Hui , Zhang Liping , Sun Jiangjie 
Published: September 2022

Abstract
Purpose: This study investigates the structure of physicians' perceived risk of physician-patient relationship from physicians' perspective, compiles the physician-patient relationship perceived risk questionnaire, provides a reliable measurement tool for quantitative research on physician-patient relationship in China, and can lay the theoretical foundation for quantitative control of physician-patient relationship risk.Methods: The initial questionnaire was formed by using literature research, expert correspondence method and applying relevant statistical analysis methods. 553 physicians were conducted to questionnaire survey, and the stability and internal consistency of the questionnaire were tested by item analysis, exploratory factor analysis and other statistical analysis.Results: Through exploratory factor analysis, we used principal component analysis and the maximum variation method in the orthogonal axis method. The final scale consisted of 4 dimensions and 16 items. The highest loadings of each factor were >0.40. The overall Cronbach's α coefficient was 0.794. All indicators of confirmatory factor analysis and the fitting test of structural model were within the standard range, indicating that the questionnaire has good construct validity. The correlation coefficients of all four factors are less than the square root value of AVE, and absolute values are all less than 0.5, indicating that the factors were correlated and differentiated from each other, which means that the scale data had good discriminant validity.Conclusion: The physician perceived risk of physician-patient relationship questionnaire developed in this study has good reliability and validity. It can be used as assessment tool to measure the perceived risk of physician in physician-patient relationship.
mRNA-LNP expressing PfCSP and Pfs25, two leading vaccine candidates targeting infection and transmission of Plasmodium falciparum
Authors: Kumar Nirbhay , Hayashi Clifford , Cao Yi , Clark Leor , Tripathi Abhai , Zavala Fidel , Dwivedi Garima , Knox James , Alameh Mohamad-Gabriel , Lin Paulo , Tam Ying , Weissman Drew 
Published: September 2022

Abstract
Malaria is a deadly disease responsible for between 550,000 and 627,000 deaths annually. There is a pressing need to develop vaccines focused on malaria elimination. The complex lifecycle of Plasmodium falciparum provides opportunities not only to target the infectious sporozoite stage, introduced by anopheline mosquitoes, but also the sexual stages, which are ingested by mosquitoes during blood feeding, leading to parasite transmission. It is widely recognized that a vaccine targeting multiple stages would induce efficacious transmission reducing immunity. Technological advancements offer new vaccine platforms, such as mRNA-LNPs, which can be used to develop highly effective malarial vaccines. We evaluated the immunogenicity of two leading P. falciparum vaccine candidates, Pfs25 and PfCSP, delivered as mRNA-LNP vaccines. Both vaccines induced extremely potent immune responses when administered alone or in combination. Purified IgGs from Pfs25 mRNA-LNPs immunized mice were highly potent in reducing malaria transmission to mosquitoes. Additionally, mice after three and four immunizations with PfCSP mRNA-LNP provided evidence for varying degrees of protection against sporozoite challenge. The comparison of immune responses and stage-specific functional activity induced by each mRNA-LNP vaccine, administered alone or in combination, also supports the development of an effective combination vaccine without any risk of immune interference for targeting malaria parasites at various life cycle stages. A combination of vaccines targeting both the infection stage and sexual/midgut stages is expected to interrupt malaria transmission, which is critical for achieving elimination goals.
Evaluating the effects of frequency and duration of tobacco use on oral mucosa in patients visiting Hamdard University Dental Hospital
Authors: Khan Obaidullah , Shaikh Abdul Hafeez , Ahmed Shaheen , Naz Shikow , Memon Raheel , Baig Qaiser Ali 
Published: September 2022

Abstract
Background
Use of tobacco in any form (Smoke or Smokeless) is one of the most popular habits around the globe that over one billion people routinely consume tobacco products like cigrates, chalia, pan, niswar (1). Almost 23.9 million Pakistani adults currently use tobacco in any form like cigarettes smoking, betel nut, betel quid, pan chalia, are all well recognized habits in developing oral lesions including precancerous and cancerous lesions like Smokers melanosis, Submucous fibrosis, Lichen Planus, Leukoplakia, Oral Squamous cell Carcinoma (OSCC) etc(2). Location and type of oral mucosal lesions varies with the way tobacco is consumed along with its frequency and duration.
Objectives
Objective of the current study is to assess the effects of frequency and duration of tobacco (Smoke and Smokeless) use on oral mucosa among patients reported at Hamdard University Dental Hospital.
Methods
The study designed as case-control and data has been collected from 280 individuals who were tobacco users categorized in groups of smokers, chewers and mixed according to their habits. Half of the subjects were assigned as cases (diagnosed with oral mucosal lesion) and the remaining half assigned as controls (who were lesion free). The study protocol includes questionnaire-based interview along with clinical examination of Oral cavity. Statistical assessment has been performed with SPSS 16.0 software guided with Chi square test and association of the variables with the lesions would be assessed through multiple logistic regression analysis.
Results
Eight different types of mucosal lesions noticed in the oral cavity among patients who have habits of smoking (cigrates), chewing (betel nuts) and mixed habits (Smoking and chewing). Out of which Leukoplakia found to be the commonest lesion within oral cavity following OSF (Oral submucous fibrosis) and smoker’s melanosis.
Conclusion
Outcomes of this study shows valuable data on association of oral lesions in patients who were involved in the habits of smoking, chewing and both (mixed). Few precancerous and cancerous conditions like oral squamous cell carcinoma were also encountered along with other mucosal lesions. Males were more prevalent in these habits than females therefore the majority of the lesions found in men. Moreover, increase in the frequency and duration of habits significantly increases the risk of developing lesions in case population.
Modelling and optimisation of ultrasound-assisted extraction of roselle phenolic compounds using the surface response method.
Authors: Sanou Abdoudramane , Konaté Kiessoun , kabakdé Kaboré , Dakuyo Roger , Bazié David , Hemayoro Sama , Dicko Mamoudou Hama 
Published: September 2022

Abstract
Extracts from Hibiscus sabdariffa L. (roselle) have been used traditionally as a food, in herbal medicine, in hot and cold beverages, as flavouring or coloring agent in the food industry. In vitro and in vivo studies and trials provide evidence, but roselle is poorly characterised phytochemically due to the extraction processes. The optimization of the extraction of phenolic compounds and their antioxidant activities is still a hot topic. In this study, the effect of solute/solvent ratio (33, 40 and 50 mg/mL), extraction temperature (40, 50 and 60°C) and extraction time (30, 60 and 90 min) was evaluated through the content of phenolic compounds and antioxidant activity. A response surface methodology through a Box-Behnken design was applied and model fit, regression equations, analysis of variance and 3D response curve were developed. The results showed that TPC, TFC, DPPH and FRAP were significantly influenced by temperature, extraction time and solvent/solute ratio. The optimal experimental condition (41.81 mg/mL; 52.35°C and 57.77 min) showed a significant positive effect compared to conventional methods. The experimental values at this extraction condition show that this optimization model is technologically, financially and energetically viable as it requires a reasonable concentration, time and temperature.
Sustainability Analysis of Sandstone Using Smart Material by EMI Approach
Authors: Kharwar Kushalendra Lal , Rawat Anupam , Srivast Rahul 
Published: September 2022

Abstract
In India, sandstone was broadly used to construct structures like Agra fort, Red Fort Delhi and Allahabad fort, etc. Around the world, many historical structures were collapsed due to the adverse effect of damages. Structural health monitoring (SHM) is very useful to take appropriate action against the failure of structure. The Electro-mechanical impedance (EMI) technique is used to continuously monitor the damage. This technique is helped to analyse the hairline crack, location, and severity of damage to structural elements. A 10cm length and 5cm diameter sandstone cylinder was used in experimental work. A cutter was used to create the artificial damages of 2mm, 3mm, 4mm, and 5mm respectively along the length, at the same place in specimens. The signature was measured for each depth of damage between 30 kHz to 400 kHz frequency range. The comparative result of healthy and damages state with different depth was concluded base on EMI signature form the sample. RMSD Statistical methods like root mean square deviation (RMSD) is used for quantification of damage. This paper motivates the application of the EMI technique to the historical building made of sand stone as key material.
Pak2 is essential for meiotic progression and meiotic apparatus assembly in mouse oocytes
Authors: Zeng Juan , Wang Shiwei , Gao Min , Lu Dian , Liu Lu , Chen Diyu , Fan Weimin , Xu Zhiliang , Sun Xiaofang 
Published: September 2022

Abstract

As a highly conserved and ubiquitously expressed serine/threonine kinase, p21-activated kinase 2 (Pak2) participates in diverse biologic events. However, its roles in mouse oocyte meiotic maturation remain unclear. The present study revealed that mouse oocytes depleted of Pak2 were unable to completely progress through meiosis and that a majority were arrested at metaphase I. Pak2 depletion thus prompted MI arrest and induced meiotic apparatus assembly defects in mouse oocytes, in part due to a reduction in polo-like kinase (Plk1). We demonstrated that Pak2’s interaction with Plk1 protected it from degradation by APC/CCdh1, and that it promoted meiotic progression and bipolar spindle formation. Our data collectively display critical functions for Pak2 in meiotic progression and meiotic apparatus assembly in mouse oocytes and may mediate the effects of maternal aging on female reproduction.
Exploring extreme thermodynamics in nanoliter volumes through stimulated Brillouin-Mandelstam scattering
Authors: Geilen Andreas , Popp Alexandra , Das Debayan , Junaid Saher , Poulton Christopher , Chemnitz Mario , Marquardt Christoph , Schmidt Markus , Stiller Birgit 
Published: September 2022

Abstract
Examining the physical properties of materials - particularly of toxic liquids - under a wide range of thermodynamic states is a challenging problem due to the extreme conditions the material has to be exposed to. Such temperature and pressure regimes, which result in a change of refractive index and sound velocity can be accessed by optoacoustic interactions such as Brillouin-Mandelstam scattering. Here we experimentally demonstrate Brillouin-Mandelstam measurements of nanoliter volumes of liquids in extreme thermodynamic regimes. We use a fully-sealed liquid-core optical fiber containing carbon disulfide; within this waveguide, which exhibits tight optoacoustic confinement and a high Brillouin gain of 32.2 ± 0.8 1/(Wm), we are able to conduct spatially resolved measurements of the Brillouin frequency shift. Knowledge of the local Brillouin response enables us to control the temperature and pressure independently over a wide range. We observe and measure the material properties of the liquid core at very large positive pressures (above 1000 bar), substantial negative pressures (below -300 bar) and we explore the isobaric and isochoric regimes.
The extensive thermodynamic control allows the tunability of the Brillouin frequency shift of more than 40% using only minute volumes of liquid. This work opens the way for future studies of liquids under a variety of conventionally hard-to-reach conditions.
Online Education Trajectory during the COVID-19 Pandemic Among the Bangladeshi Adolescent Children
Authors: Sakib Farhan 
Published: September 2022

Abstract

Bangladesh adopted both cutting-edge technology and the pandemic almost simultaneously. Because of this, the government and other groups were able to respond quickly to the evolving situation. Although many sectors, including education, went through a difficult adjustment, the process of adapting was relatively swift. There was an attempt to implement the "New Normal". Teachers and educational institutions modified their methods of instruction. But because of a shortage of resources, it wasn't always advantageous for the students. It may be possible to reduce the vulnerabilities of the online learning that started during the pandemic through strategic ways, including introducing a new policy for a defined amount of time and involving community members. The cooperation of multiple stakeholders will make it easier to identify any potential flaws and difficulties in Bangladesh's online education system. Community members can make sure to be present in every aspect of the online and offline classes, including serving as a channel for parent-teacher communication, keeping up with local administration, and organizing volunteer and social awareness campaigns.
Preference of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae) for weed and cultivated species
Authors: Sacilotto Matheus Gerage , Souza Felipe Savieto Furquim , Baldin Edson Luiz Lopes , Carbonari Caio Antonio , Lourenção André Luiz , Faria Rodrigo Donizeti 
Published: September 2022

Abstract

Being capable of infesting a wide variety of plant species, the whitefly Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae) is responsible for severe losses in numerous agricultural crops. In order to increase knowledge regarding interactions involving B. tabaci MEAM1 and plants associated with agricultural landscapes, the present study sought to identify preferential hosts by comparing 15 different common weed species and five cultivated plants (tomato, bell pepper, soybean, maize and cotton) through free and no-choice tests. Additionally, a possible correlation between physical-morphological plant aspects and insect’s colonization behavior was assessed. Positive correlations were verified between the oviposition index and trichome density, and between the number of adults and b* (yellow intensity) index. Negative correlations were observed between the number of adults and L* and a* (luminosity and green intensity, respectively) indexes. In the free choice test, the species Solanum lycopersicum, Senna obtusifolia, Glycine max, Emilia sonchifolia and Euphorbia heterophylla were the most infested during the mean of the evaluation periods, differing from Spermacoce latifolia, Amaranthus viridis and Richardia brasiliensis, which presented the lowest means of insect infestation. In this same test, S. lycopersicum and E. sonchifolia had the greatest oviposition, differing from most of the species. In the no-choice test, E. heterophylla, Galinsoga parviflora and S. latifolia had the highest means of eggs and nymphs per cm². Our results show evidence of the expressive potential of weed species frequently found in Brazilian agricultural fields, such as E. sonchifolia, S. obtusifolia, and E. heterophylla, as alternative hosts of B. tabaci MEAM1.
Effect of replacing soybean meal (Glycine max) with sesame meal (Sesamum indicum) in fattening lamb’s diets
Authors: Trejo Eleazar Pérez , Montemayor Héctor M. Andrade , Jimenez Lizbeth E Robles , Humarán María Concepción Méndez Gómez , Estrada Elba Orozco , Hernandez Jesús Hernández , VARGAS-BELLO-PEREZ EINAR , Ronquillo Manuel González 
Published: September 2022

Abstract

The objective of this study was to determine the effect of replacing soybean meal (Gly-cine max) with sesame meal (Sesamum indicum) in fattening lamb’s diets. For this, 44 Katahdin lambs were divided into three treatments in duplicate: basal diet + soybean meal (100S), basal diet + sesame meal / soybean meal (50 / 50SA) and basal diet + sesame meal (100A). Dry matter intake, daily weight gain (DWG), total kg gained (KgT), feed conversion (FC) and feed efficiency (FE) were evaluated, upon reaching the weight for sale, the animals were slaughtered, and hot carcass weight (HCW) was evaluated. The results were analyzed with a completely randomized design with repeated measures. Regarding time, no differences were found between treatments, for DWG (0.171 ± 0.006 kg/d), FC (6.7 ± 0.55), EA (0.175 ± 0.02), KgT (2.86 ± 0.13), RCC (50.97 ± 0.79), as well as for chest depth (26.96 ± 0.33), leg width (20.63 ± 0.028), leg diameter (60.7 ± 0.44), and ribs width (24.05 ± 0.14). Gross profitability margin (MUB) was 16.50%, 18.63%, 19.97% for 100S, 50 / 50SA and 100A respectively. Overall, in fatting lamb diets, replacing soybean meal with sesame meal by either 50% or 100% substitution could be a feasible feeding strategy as in both cases, gross profit was increased, and no negative effects were found for productive traits and carcass quality.
Carnitine palmitoyltransferase 1A promotes mitochondrial fission and regulates autophagy by enhancing MFF succinylation in ovarian cancer
Authors: Shao Huanjie , Zhu Yaqin , Wang Yue , Li Ying , Li Zhongqi , Kong Wenhui , Zhao Xiaoxuan , Chen Shuting , Yan Liting , Wang Lenan , Tong Yunli 
Published: September 2022

Abstract
Mitochondria are dynamic organelles and important for cell growth and proliferation. Dysregulated mitochondrial dynamics are highly associated with the initiation and progression of various cancers, including ovarian cancer. However, the regulatory mechanism underlying mitochondrial dynamics is still unclear and needs to be further studied. Previously, our study showed that Carnitine palmitoyltransferase 1A (CPT1A) is highly expressed in ovarian cancer cells and promotes the development of ovarian cancer. Here, we found that CPT1A regulates mitochondrial dynamics and promotes mitochondrial fission in ovarian cancer cells. In addition, autophagy induced by CPT1A knockdown was also related to mitochondrial dynamics. Further study showed that CPT1A regulates mitochondrial fission and function through MFF to promote the growth and proliferation of ovarian cancer cells. Mechanistically, CPT1A promotes succinylation of MFF at lysine 302 (K302), which protects against Parkin-mediated ubiquitin-proteasomal degradation of MFF. Finally, the study showed that MFF was highly expressed in ovarian cancer cells and that high MFF expression is associated with poor prognosis in patients with ovarian cancer. MFF inhibition significantly inhibited the progression of ovarian cancer in vivo. Together, CPT1A regulates mitochondrial dynamics through MFF succinylation to promote the progression of ovarian cancer. And MFF is a potential therapeutic target for ovarian cancer.
Inhibition of CDK4/6 and BET suppresses pancreatic tumor growth and epithelial-to-mesenchymal transition independently of PI3K-AKT by regulating phosphorylation of GSK3β induced Wnt/β-catenin pathway
Authors: Gu Jiangning , Chen Xiang , Chen Dan , Duan Qingzhu , Yin Menghong , Qi Wenjing , Peng Chenghong , Chen Hao , Li Ying , Zhu Youwei , Wei Gang , Wang Chenqi , Xu Shiqi , Tan Guang , Wang Xiuli , Luo Haifeng 
Published: September 2022

Abstract

The cyclin-dependent kinases, CDK4 and CDK6 are upregulated in various cancers, including pancreatic ductal adenocarcinoma. However, monotherapy with CDK4/6 inhibitors has been unsatisfactory and several oncogenic pathways might be activated. We demonstrated that PD-0332991, an FDA-approved CDK4/6 inhibitor, moderately suppressed tumor growth and induced the epithelial-to-mesenchymal transition (EMT). However, the performance of this inhibitor was significantly improved and the EMT partly reversed when bromodomain and extra-terminal motif (BET) was inhibited by JQ1. Mechanistically, CDK4/6 inhibition activated the canonical Wnt/β-catenin pathway through GSK3β Ser9 phosphorylation. Crosstalk between the TGFβ/Smad and Wnt/β-catenin pathways based on GSK3β was disrupted by JQ1 and levels of active β-catenin were suppressed by regulating the phosphorylation status of GSK3β. The simultaneous inhibition of CDK4/6 and BET synergistically suppressed tumor growthin vitroandin vivo. Moreover, CDK4 and CDK6 negatively correlated with overall survival, and the predictive accuracy of a CDK4/6-based nomogram was higher than that of tumor size, nodes, and metastasis (TNM) classification alone. Our findings provide theoretical evidence for the construction of predictive models and new targets with which to treat pancreatic cancer.
Feasibility of home initiation of subcutaneous apomorphine infusion for patients with Parkinson’s Disease
Authors: Zagnoli Fabien , Leblanc Amelie , Viakhireva-Dovganyuk Irina , Delabrousse-Mayoux Jean-Philippe , Pouyet Alain , Ziegler Marc , Sogni Laura , Patat Marie , Bouillot Regis , Verin Marc 
Published: September 2022

Abstract
Background: Continuous subcutaneous apomorphine infusion (CSAI) is increasingly being used to treat patients with Parkinson’s disease who are experiencing motor fluctuations. However, the need to initiate this treatment during a hospital stay, more often than not in a specialist center, may restrict patients’ access to it. Objective: To assess the feasibility and benefits of initiating CSAI in the patient’s own home. Method: A prospective multicenter longitudinal observational study (APOKADO Study) among patients with Parkinson’s disease who required subcutaneous apomorphine, comparing in-hospital versus home initiation. We assessed patients’ quality of life with the 8-item Parkinson’s Disease Questionnaire, rated the improvement in their clinical status on the 7-point Clinical Global Impression – Improvement scale, recorded adverse events, and ran a cost benefit analysis. Results: Participants were145 patients with motor fluctuations (mean age: 70 years; mean disease duration: 11.1 years). Of these, 106 (74%) were initiated onto CSAI at home, and 38 (26%) in hospital. At inclusion, the two groups were comparable on all demographic and clinical characteristics. Quality of life improved more quickly in the home group than in the hospital one, and the percentages of patients whose clinical status was much or very much improved were also higher. Adverse events and early dropout rates were similar and similarly rare across the two groups. Patients in the home group became more autonomous in managing the device than those in the hospital group, and their care cost less. Conclusion: This study shows that home (versus in-hospital) initiation of CSAI is feasible, improves patients’ quality of life more and more quickly, with a greater clinical benefit and exactly the same level of tolerance. It is also less expensive. This finding should make it easier for patients to access this treatment in the future.