bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes of bile acids and resting energy expenditure after laparoscopic cholecystectomy in type 2 diabetes patients: a prospective study
Authors: Yin Haixin , Chen Weijie , He Xiaodong , Zeng Jianping 
Published: July 2022

Abstract
Background
We aimed to investigate changes of bile acids and resting energy expenditure (REE) in patients with type 2 diabetes mellitus (T2DM) after laparoscopic cholecystectomy (LC) and the role in metabolic homeostasis.

Methods
From December 2019 to December 2021, a total of 77 T2DM patients with gallbladder polyps were included in our study. Among them, 40 patients who underwent LC were enrolled into the cholecystectomy group, and 37 patients who did not undergo LC were enrolled into the control group. Preoperative and 6-months postoperative demographic data, body weight, food intake, effects on diabetes control, and biomedical variables were recorded and compared.

Results
The mean level of total bile acids (TBA) was higher than that in the control group (P = 0.033) and increased significantly after LC compared to baseline (P = 0.029). The REE level in the cholecystectomy group was higher than that in the control group (P = 0.032) and increased compared to the baseline (P = 0.011). The utilization of carbohydrates increased significantly after LC (P < 0.001) while the utilization of fat decreased (P < 0.001). The mean level of fasting plasma glucose (P = 0.004), hemoglobin A1C (P < 0.001), and homeostasis model assessment-insulin resistance (P = 0.045) decreased after LC. The mean level of total cholesterol (P = 0.003) and low-density lipoprotein cholesterol significantly decreased (P = 0.021), whereas the level of high-density lipoprotein cholesterol increased (P < 0.001).

Conclusions
The level of REE and TBA increased after LC in patients with T2DM, and the glucose and lipid metabolism improved.
Trial registration This study was registered in the Chinese Clinical Trial Registry on November 30, 2018, registered number: ChiCTR1900027823.
Whey protein improves glycemia during an oral glucose tolerance test compared to vigorous-intensity aerobic exercise in young adult men
Authors: Gordon Ryan A. , Zumbro Emily L. , Castleberry Todd J. , Sokoloski Matthew L. , Brisebois Matthew F. , Irvine Christopher J. , Duplanty Anthony A. , Ben-Ezra Vic 
Published: July 2022

Abstract
Background
Both aerobic exercise and whey protein can improve glucose regulation. The purpose of this study was to investigate how a single bout of vigorous-intensity aerobic exercise and whey protein, independently, as well as when combined, influence glycemia during an oral glucose tolerance test in sedentary, young men.

Methods
Healthy males (n = 11) completed four randomized trials: no exercise/no whey protein (R); exercise (EX; walking at 70% VO2max for 60 min); 50 g of whey protein (W); and exercise combined with 50 g of whey protein (EXW). Each trial included a 75 g oral glucose tolerance test (OGTT) that was completed after an overnight fast. Blood samples were collected over a two-hour period during the OGTT. For EX and EXW, the exercise was performed the evening before the OGTT and the 50 g of whey protein was dissolved in 250 mL of water and was consumed as a preload 30 min prior to the OGTT. For R and EX, participants consumed 250 mL of water prior to the OGTT. Plasma samples were analyzed for glucose, insulin, C-peptide, glucagon, gastric inhibitory peptide (GIP) and glucagon like peptide 1 (GLP-1), and postprandial incremental area under the curve (iAUC) was calculated for each.

Results
Glucose iAUC was reduced during W (− 32.9 ± 22.3 mmol/L) compared to R (122.7 ± 29.8 mmol/L; p < 0.01) and EX (154.3 ± 29.2 mmol/L; p < 0.01). Similarly, glucose iAUC was reduced for EXW (17.4 ± 28.9 mmol/L) compared to R and EX (p < 0.01 for both). There were no differences in iAUC for insulin, C-peptide, GIP, GLP-1, and glucagon between the four trials. Insulin, C-peptide, glucagon, GIP, and GLP-1 were elevated during the whey protein preload period for W and EXW compared to EX and R (p < 0.01). There were no differences for insulin, C-peptide, glucagon, GIP, or GLP-1 between trials for the remaining duration of the OGTT.

Conclusions
Glucose responses during an oral glucose tolerance test were improved for W compared to EX. There were no additional improvements in glucose responses when vigorous-intensity aerobic exercise was combined with whey protein (EXW).
On new sixth and seventh order iterative methods for solving non-linear equations using homotopy perturbation technique
Authors: Thota Srinivasarao , Shanmugasundaram P. 
Published: July 2022

Abstract
Objectives
This paper proposes three iterative methods of order three, six and seven respectively for solving non-linear equations using the modified homotopy perturbation technique coupled with system of equations. This paper also discusses the analysis of convergence of the proposed iterative methods.

Results
Several numerical examples are presented to illustrate and validation of the proposed methods. Implementation of the proposed methods in Maple is discussed with sample computations.
Impact of acute mental stress on ankle blood pressure in young healthy men: a pilot study
Authors: Kume Daisuke , Nishiwaki Masato , Hotta Norio , Endoh Hiroshi 
Published: July 2022

Abstract
Objective
Acute mental stress (MS) increases arm blood pressure (BP); however, it remains unclear whether a stress-induced pressor response is also observed in other vessels. This study aimed to examine the impact of acute MS on ankle BP. Fifty-six young, healthy men aged 19–24 years were divided into the MS (n = 29) and control (CON) (n = 27) groups; each group performed 5-min MS (mental arithmetic) or CON tasks. Systolic and diastolic BPs (SBP and DBP, respectively) of both the brachial and posterior tibial arteries were simultaneously measured at the baseline and 5 and 30 min after the task.

Results
In the MS group, brachial BP measures significantly increased (P < 0.05) until 30 min after the task; ankle BP measures were also significantly (P < 0.05) elevated during this time. In the CON group, no significant changes were found in brachial BP measures or ankle SBP, whereas a significant increase (P < 0.05) in ankle DBP was observed 30 min after the task. Our findings indicate that both brachial and ankle BP exhibit a sustained elevation after acute MS, suggesting a systemic pressor response by stress exposure. The measurement of ankle BP in addition to arm BP may be important to assess the stress response.

Trial Registration
UMIN Clinical Trials Registry UMIN000047796 Registered on: 20th May 2022.
Optimizing artificial meniscus by mechanical stimulation of the chondrocyte-laden acellular meniscus using ad hoc bioreactor
Authors: Shadi Mehri , Talaei-Khozani Tahereh , Sani Mahsa , Hosseinie Radmarz , Parsaei Hossein , Vojdani Zahra 
Published: July 2022

Abstract
Background
Tissue engineering focuses on reconstructing the damaged meniscus by mimicking the native meniscus. The application of mechanical loading on chondrocyte-laden decellularized whole meniscus is providing the natural microenvironment. The goal of this study was to evaluate the effects of dynamic compression and shear load on chondrocyte-laden decellularized meniscus.

Material and methods
The fresh samples of rabbit menisci were decellularized, and the DNA removal was confirmed by histological assessments and DNA quantification. The biocompatibility, degradation and hydration rate of decellularized menisci were evaluated. The decellularized meniscus was injected at a density of 1 × 105 chondrocyte per scaffold and was subjected to 3 cycles of dynamic compression and shear stimuli (1 h of 5% strain, ± 25°shear at 1 Hz followed by 1 h rest) every other day for 2 weeks using an ad hoc bioreactor. Cytotoxicity, GAG content, ultrastructure, gene expression and mechanical properties were examined in dynamic and static condition and compared to decellularized and intact menisci.

Results
Mechanical stimulation supported cell viability and increased glycosaminoglycan (GAG) accumulation. The expression of collagen-I (COL-I, 10.7-folds), COL-II (6.4-folds), aggrecan (AGG, 3.2-folds), and matrix metalloproteinase (MMP3, 2.3-folds) was upregulated compared to the static conditions. Furthermore, more aligned fibers and enhanced tensile strength were observed in the meniscus treated in dynamic condition with no sign of mineralization.

Conclusion
Compress and shear stimulation mimics the loads on the joint during walking and be able to improve cell function and ultrastructure of engineered tissue to recreate a functional artificial meniscus.
Tendinopathy: sex bias starts from the preclinical development of tendon treatments. A systematic review
Authors: Mondini Trissino da Lodi Camilla , Salerno Manuela , Merli Giulia , Brama Pieter , Jenner Florien , Filardo Giuseppe 
Published: July 2022

AbstractTendinopathies are common overuse disorders that arise both in athletes and the general population. Available tendon treatments are used both for women and men without distinction. However, the existence of a sex-based difference in tendon biology is widely demonstrated. Since basic research represents the foundation for treatment development, an equal female–male representation should be pursued in preclinical studies. This systematic review quantified the current evidence by analyzing 150 studies on 8231 animals. Preclinical studies largely neglected the importance of sex, none analyzed sex-based differences, and only 4% of the studies reported disaggregated data suitable for the analysis of treatment results in males and females. There is an alarming female under-representation, in particular in the field of injective therapies. Despite the growing awareness on the importance of investigating treatments in both males and females, the investigated field proved resistant from properly designing studies including both sexes, and the lack of sex-representation remains critical.
Characterization of symbiotic and nitrogen fixing bacteria
Authors: Kawaka Fanuel 
Published: July 2022

AbstractSymbiotic nitrogen fixing bacteria comprise of diverse species associated with the root nodules of leguminous plants. Using an appropriate taxonomic method to confirm the identity of superior and elite strains to fix nitrogen in legume crops can improve sustainable global food and nutrition security. The current review describes taxonomic methods preferred and commonly used to characterize symbiotic bacteria in the rhizosphere. Peer reviewed, published and unpublished articles on techniques used for detection, classification and identification of symbiotic bacteria were evaluated by exploring their advantages and limitations. The findings showed that phenotypic and cultural techniques are still affordable and remain the primary basis of species classification despite their challenges. Development of new, robust and informative taxonomic techniques has really improved characterization and identification of symbiotic bacteria and discovery of novel and new species that are effective in biological nitrogen fixation (BNF) in diverse conditions and environments.
Oxysterol derivatives Oxy186 and Oxy210 inhibit WNT signaling in non-small cell lung cancer
Authors: Tang Liu-Ya , Spezia Marie , Chen Ting , Shin Jee-Hye , Wang Feng , Stappenbeck Frank , Lebensohn Andres M. , Parhami Farhad , Zhang Ying E. 
Published: July 2022

Abstract
Background
Developmental signaling pathways such as those of Hedgehog (HH) and WNT play critical roles in cancer stem cell self-renewal, migration, and differentiation. They are often constitutively activated in many human malignancies, including non-small cell lung cancer (NSCLC). Previously, we reported that two oxysterol derivatives, Oxy186 and Oxy210, are potent inhibitors of HH/GLI signaling and NSCLC cancer cell growth. In addition, we also showed that Oxy210 is a potent inhibitor of TGF-β/SMAD signaling. In this follow-up study, we further explore the mechanism of action by which these oxysterols control NSCLC cell proliferation and tumor growth.

Results
Using a GLI-responsive luciferase reporter assay, we show here that HH ligand could not mount a signaling response in the NSCLC cell line A549, even though Oxy186 and Oxy210 still inhibited non-canonical GLI activity and suppressed the proliferation of A549 cells. Further, we uncover an unexpected activity of these two oxysterols in inhibiting the WNT/β-catenin signaling at the level of LRP5/6 membrane receptors. We also show that in a subcutaneous xenograft tumor model generated from A549 cells, Oxy186, but not Oxy210, exhibits strong inhibition of tumor growth. Subsequent RNA-seq analysis of the xenograft tumor tissue reveal that the WNT/β-catenin pathway is the target of Oxy186 in vivo.

Conclusion
The oxysterols Oxy186 and Oxy210 both possess inhibitory activity towards WNT/β-catenin signaling, and Oxy186 is also a potent inhibitor of NSCLC tumor growth.
Determinants of adoption of climate-smart agricultural technologies and practices in the coffee-based farming system of Ethiopia
Authors: Diro Samuel , Tesfaye Agajie , Erko Beza 
Published: July 2022

Abstract
Objectives
This study explored the adoption status of different Climate Smart Agricultural (CSA) practices and factors that influence their adoption for sustainable soil resource utilization in the changing climate.

Methodology
We used quantitative and qualitative primary data collected from smallholder farmers and other stakeholders from major coffee-growing regions of Ethiopia: Oromia, and Southern Nations, Nationalities, and Peoples (SNNP). We used the multivariate probit (MVP) model to study factors that influence the adoption of climate-smart agricultural technologies, namely, manure application, minimum tillage, intercropping, use of improved forage, and physical soil and water management practices.

Results
The study result shows that 35% of farmers apply manure on their farm plots. Minimum tillage is also applied to 36% of farms. Intercropping improved forages and physical soil and water management structures are adopted by 45, 19, and 47% of farmers, respectively. The finding of the study indicates the positive and significant effect of education, extension (access to extension services and participation on field days), and ownership of communication devices specifically radio on the adoption of climate-smart agricultural practices.

Recommendations
Concerning bodies must pay due attention to problems affecting effective farmers-extension linkage. The positive effect of radio ownership on technology adoption also suggests the need for increased accessibility of FM radio channels to farmers to be aware of climate change and innovative agricultural technologies, practices, and information that mitigate the problem.
Host-microbiota interaction-mediated resistance to inflammatory bowel disease in pigs
Authors: Zhao Xuan , Jiang Lin , Fang Xiuyu , Guo Zhiqiang , Wang Xiaoxu , Shi Baoming , Meng Qingwei 
Published: July 2022

Abstract
Background
Disease resistance phenotypes are associated with immune regulatory functions and immune tolerance and have implications for both the livestock industry and human health. Microbiota plays an essential role in regulating immunity and autoimmunity in the host organism, but the influence of host-microbiota interactions on disease resistance phenotypes remains unclear. Here, multiomics analysis was performed to identify potential regulatory mechanisms of disease resistance at both the microbiome and host levels in two pig breeds.

Results
Acute colitis models were established in Min pigs and Yorkshire pigs, and control and diseased individuals were compared. Compared with Yorkshire pigs under the same nutritional and management conditions, Min pigs exhibited strong disease resistance, as indicated by a low disease activity index (DAI) and a low histological activity index (HAI). Microbiota sequencing analysis showed that potentially harmful microbes Desulfovibrio, Bacteroides and Streptococcus were enriched in diseased individuals of the two breeds. Notably, potentially beneficial microbes, such as Lactobacillus, Clostridia and Eubacterium, and several genera belonging to Ruminococcaceae and Christensenellaceae were enriched in diseased Min pigs and were found to be positively associated with the microbial metabolites related to intestinal barrier function. Specifically, the concentrations of indole derivatives and short-chain fatty acids were increased in diseased Min pigs, suggesting beneficial action in protecting intestinal barrier. In addition, lower concentrations of bile acid metabolites and short-chain fatty acids were observed in diseased Yorkshire pigs, which were associated with increased potentially harmful microbes, such as Bilophila and Alistipes. Concerning enrichment of the immune response, the increase in CD4+ T cells in the lamina propria improved supervision of the host immunity response in diseased Min pigs, contributing to the maintenance of Th2-type immune superiority and immune tolerance patterns and control of excessive inflammation with the help of potentially beneficial microbes. In diseased Yorkshire pigs, more terms belonging to biological processes of immunity were enriched, including Toll-like receptors signalling, NF-κB signalling and Th1 and Th17-type immune responses, along with the increases of potentially harmful microbes and damaged intestinal barrier.

Conclusions
Cumulatively, the results for the two pig breeds highlight that host-microbiota crosstalk promotes a disease resistance phenotype in three ways: by maintaining partial PRR nonactivation, maintaining Th2-type immune superiority and immunological tolerance patterns and recovering gut barrier function to protect against colonic diseases.
A Lagrangian–Eulerian procedure for the coupled solution of the Navier–Stokes and shallow water equations for landslide-generated waves
Authors: Masó Miguel , Franci Alessandro , de-Pouplana Ignasi , Cornejo Alejandro , Oñate Eugenio 
Published: July 2022

AbstractThis work presents a partitioned method for landslide-generated wave events. The proposed strategy combines a Lagrangian Navier Stokes multi-fluid solver with an Eulerian method based on the Boussinesq shallow water equations. The Lagrangian solver uses the Particle Finite Element Method to model the landslide runout, its impact against the water body and the consequent wave generation. The results of this fully-resolved analysis are stored at selected interfaces and then used as input for the shallow water solver to model the far-field wave propagation. This one-way coupling scheme reduces drastically the computational cost of the analyses while maintaining high accuracy in reproducing the key phenomena of the cascading natural hazard. Several numerical examples are presented to show the accuracy and robustness of the proposed coupling strategy and its applicability to large-scale landslide-generated wave events. The validation of the partitioned method is performed versus available results of other numerical methods, analytical solutions and experimental measures.
Barriers to medications for opioid use disorder in the court system: provider availability, provider “trustworthiness,” and cost
Authors: Ahmed Fatema Z. , Andraka-Christou Barbara , Clark M.H. , Totaram Rachel , Atkins Danielle N. , del Pozo Brandon 
Published: July 2022

Abstract
Background
Medications for opioid use disorder (MOUD) significantly decrease mortality but courts rarely refer participants with opioid use disorder to MOUD providers. Previous qualitative work suggests routine court referrals to MOUD providers are more likely if court team members perceive providers as “trustworthy.” Court team members may also be less likely to refer participants to MOUD if they consider MOUD unaffordable, particularly in Florida, which has not expanded Medicaid. Our aims were to explore court team members’ 1) perceptions of availability of local trustworthy MOUD providers, 2) characteristics associated with perceptions of availability of local trustworthy MOUD providers, including beliefs about MOUD efficacy, and 3) perceptions of MOUD affordability.

Methods
An online survey was distributed to all criminal problem-solving court and dependency court team members in Florida in 2019 and 2020. Likert scale questions assessed respondent agreement with statements about the availability of any MOUD providers, the availability of trustworthy MOUD providers, and the affordability of MOUD for court participants. An open-ended question explored MOUD barriers. Spearman’s rho, Friedman, Kruskal Wallis, and Mann-Whitney U tests were used for analyzing quantitative data and iterative categorization for qualitative data.

Results
One hundred fifty-one respondents completed quantitative questions (26% response rate), and 42 completed the qualitative question. Respondents were more likely to agree that local MOUD providers are more available than trustworthy MOUD providers. Perceptions of trustworthy provider availability differed significantly by MOUD type and were associated with MOUD efficacy beliefs. Qualitative results suggest that MOUD providers offering counseling and individualized treatment are more trustworthy.

Conclusions
Court team MOUD beliefs may influence their perceptions of providers, or negative experiences with providers may influence court team MOUD beliefs. Improving court team perceptions of local MOUD providers may be critical for facilitating court participant treatment access.
End of the road? The career intentions of under-represented STEM students in higher education
Authors: Wong Billy , Chiu Yuan-Li Tiffany , Murray Órla Meadhbh , Horsburgh Jo 
Published: July 2022

Abstract
Background
The analogy of the leaky pipeline has been used to describe STEM education, with lower student diversity from compulsory to post-compulsory education and beyond. Although extensive research has explored the views and experiences of school-aged children about STEM, fewer studies have examined the career intentions of STEM students at university, especially those from under-represented backgrounds (e.g., racial/ethnic minority, women and working class students). This paper draws on a large qualitative study that interviewed 110 under-represented STEM undergraduates in the UK. We focus on students’ STEM career intentions and the likely directions of their post-degree trajectories, drawing on the lenses of science identity and Social Cognitive Career Theory.

Results
Three pathways were identified. The first group plans to pursue a career in or from STEM. While social inequalities may persist, the potential impact of these challenges may be neutralised by the personal drive and passion of STEM career-oriented students, who seem committed to drive into an STEM future. The second group stated intentions for non-STEM-related careers, leaving the STEM pipeline. The reasons students gave for their imminent departure from STEM are the better financial reward on offer in some non-STEM sectors, especially in finance and business, as well as wider social inequalities and stereotypes. The third group was undecided, those who are uncertain or unclear about their futures. Students described a general lack of direction or clear career pathway, from a complete lack of career ideas to an overload of options.

Conclusions
We conclude with a reminder that the STEM pipeline is far from secured or equitable, despite apparent progress in participation and representation. We reiterate the importance of fostering a diverse, inclusive and supportive learning environment that maximises the participation, strengths and potential of all students, especially those from under-represented backgrounds. While it is not uncommon for STEM students to pursue careers outside of STEM, we need to be wary that those who exit the STEM pipeline are not forced off the road by social inequalities and exclusions.