bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cancer stem-like cells of nasopharyngeal carcinoma express CXCR4 and display highly invasive activity
Authors: Zhu Zhenwei , Li Jingyu , Liu Jingxian , He Yuan , Xue Yan , Hong Xiangchan , Jiang Zeying , Luo Dangu , Guo Pei , Yao Kaitai , Chen Lin 
Published: August 2022

Abstract
Background: Nasopharyngeal carcinoma (NPC) patients with distant metastasis have a poor response to conventional first-line chemoradiotherapy and a poor prognosis. Growing evidence suggests that a subpopulation of cancer stem cells (CSCs) with certain markers is essential for cancer metastasis. However, the unique subset of CSCs that drives NPC metastasis is still unclear. Methods: Immunohistochemistry was used to analyze the expression characteristics, distribution and relationship of CXCR4 with clinical parameters in nasopharyngeal carcinoma. The expression characteristics of CD133 and CXCR4 in nasopharyngeal carcinoma tumorspheres were analyzed by flow cytometry. Through a magnetic activated cell sorting system, CXCR4-positive and CXCR4-negative tumorsphere cells were obtained, and then, the differences in invasion (in vitro and in vivo), metastatic tumor formation, stemness and EMT characteristics of the two cell types were compared. SDF-1 and its inhibitor were used to explore the roles of stromal-cell derived factor-1 (SDF-1) in the metastatic process. Results: In 71 cases of human nasopharyngeal carcinoma, CXCR4 expression was correlated with T stage and N stage. The CXCR4-positive rate of the tumor cells in the T3-4 group was higher than that in the T1-2 group (60.6% vs 28.9%, P =0.009), and that in the N2-3 group was higher than that in the N0-1 group (76.5% vs 13.5%, P=0.000). The CXCR4 expression level was higher in cells with high metastasis or low differentiation. The culture of tumor spheres enriched CD133-positive and CXCR4-positive cells, and most CD133-positive cells also expressed CXCR4 in NPC. The secondary tumorsphere-forming rate of the CXCR4-positive tumorsphere cells was higher than that of the CXCR4-negative tumorsphere cells, and most of the CXCR4-positive tumorsphere cells had a spindle morphology. The CXCR4-positive tumorsphere cells had stronger migration and invasion ability in vitro and metastatic tumor formation ability in mice, which depends on the SDF-1/CXCR4 axis, than the CXCR4-negative tumorsphere cells.Compared with those of the CXCR4-negative tumorsphere cells, the mRNA expression levels of Oct4, Nanog, Sox2, Snail, Twist, Vimentin and N-cadherin in the CXCR4-positive tumor tumorsphere cells were increased, while E-cadherin decreased, and the protein expression levels of Vimentin and N-cadherin increased, while that of E-cadherin decreased. Conclusion: Therefore, CXCR4 may be a marker of nasopharyngeal metastatic tumor stem cells, and CXCR4 may be involved in tumor stem cell migration and invasion by regulating the EMT process. These results suggest that targeting the SDF1/CXCR4 axis may interfere with nasopharyngeal carcinoma tumor stem cell migration and invasion and provide new treatment strategies.
Do Executive Functions Predict Physical Activity Behavior? – A Meta-Analysis
Authors: Gürdere Ceren , Strobach Tilo , Pastore Massimiliano , Pfeffer Ines 
Published: August 2022

Abstract
Background: Executive functions (EFs) are important determinants of health behaviors. In the present study, a meta-analysis was conducted to investigate the relationship between EFs and physical activity (PA) behavior. Methods: Systematic searches were carried out and prospective studies which reported the relationship between baseline EFs and later PA behavior were selected. Eight studies were found eligible. Results: Results of the multilevel meta-analysis revealed a significant total effect size for EFs on PA behavior. High heterogeneity was observed among studies. When potential moderators were tested, residual heterogeneity remained significant and the effects of the moderators were not significant. The effect size dropped when accounted for publication bias. Conclusions: Despite limitations, the study provided evidence for EFs’ determinant role on PA behavior. More research is however encouraged to inform PA promotion programs that are well-prepared for individual differences in EFs.
Monosodium glutamate (MSG) perturbs trophoblast differentiation and invasion program through a ROS-mediated pathway
Authors: Mukherjee Indrani , Singh Sunil , Biswas Subhrajit , Talukdar Joyeeta , Alqahtani Mohammed S. , Abbas Mohamed , Nag Tapas Chandra , Mridha Asit , Gupta Surabhi , Sharma Jai Bhagwan , Kumari Supriya , Dhar Ruby , Karmakar Subhradip 
Published: August 2022

Abstract
Monosodium glutamate (MSG) is a flavor-enhancing food additive widely used in Asian cuisine. It is one of the common ingredients used in curry and other tinned food that is believed to bring out the "umami" meat flavor and enhance the savory taste of the food. It is the sodium salt of the naturally occurring amino acid Glutamate. Due to its massive consumption as part of fast-food culture along with concerns about its possible effect on health in general and pregnancy in particular, we undertook this study to address its impact on placental trophoblast cells. We investigated the effect of MSG on placental trophoblast cells. Our studies confirmed that short and long-term exposure to MSG has a profound influence on trophoblast invasion and differentiation, two of the most critical and dispensable functions during placentation. Our results showed an acute short-term MSG-treatment enhanced trophoblast invasion and cell motility along with an increased expression in trophoblast cell differentiation markers. However, long-term chronic MSG stimulation in these cell types showed the opposite effect with both reduced invasion and differentiation. Further, MSG-treated placental explants displayed signs of elevated oxidative stress. These effect of MSG on trophoblast cells along with an increased ROS production indicates that long-term usage of MSG as part of food additive might have adverse health consequences on the developing embryo by compromising the trophoblast and placental function.
Association of preterm outcome with maternal systemic lupus erythematosus: a retrospective cohort study
Authors: Chen Xiafang , Di Wen , Ye Lili , Hu Yabin , Jiang Meng , Wu Jiayue , Bu Jun , Sun Jianhua , Bei Fei 
Published: August 2022

Abstract
Background
Maternal systemic lupus erythematosus (SLE) is at greater risk of pregnancy complications and is associated with increased risk of preterm delivery. However hardly any study has looked at the influence of SLE on the outcomes of preterm infants. This study aimed to explore the influence of SLE on the outcomes of preterm infants.
Methods
In this retrospective cohort study, preterm infants born to mothers with SLE from Shanghai Children's Medical Center during 2012 to 2021 were enrolled. Infants were excluded if they were died during hospitalization or has major congenital anomalies and neonatal lupus. Exposure was defined as mother diagnosed SLE before or during pregnancy. Maternal SLE group was matched with Non-SLE group by gestational age, birth weight and gender. Clinical data has been extracted from patients’ records and registered. Major morbidities of premature and biochemical parameters in the two groups were compared using multiple logistic regression༎
Results
One hundred preterm infants born to 95 mothers with SLE were finally enrolled. The mean (standard deviation) of gestational age and birth weight were 33.09 (7.28) weeks and 1768.50 (423.56) g respectively. There was no significant difference in major morbidities between SLE group and non-SLE group. Compared with non-SLE group, SLE off-spring had significantly lower leukocytes, neutrophiles after birth, neutrophils and platlet in one week (mean difference: -2.825, -2.001, -0.842, -45.469, respectively). Among SLE group, lower birth weight and smaller gestational age were observed in SLE mothers with disease active during pregnancy, kidney involved, blood system involved and not taking Aspirin during pregnancy. Multivariable logistic regression analysis indicated that taking aspirin during pregnancy was associated with very preterm birth and survive without major morbidities.
Conclusion
Born to mothers with SLE may not increase the risk of major premature morbidities, but the hematologic profile of SLE preterm infants may be different from preterm infants born to women without SLE. The outcome of SLE preterm infants is associated with maternal SLE status and may benefit from maternal aspirin administration.
EHTT2022-Prediction of grinding force by an electroplated grinding wheel with orderly-micro-grooves
Authors: Mao Cong , Wang Jiali , Zhang Mingjun , Wang Xincheng , Luo Yuanqiang , Tang Weidong , Tang Kun , Bi Z.M. , Hu Yongle , Lin Zhenheng 
Published: August 2022

Abstract
The ability of predicting a grinding force is important to control, monitor, and optimize grinding process. Few theoretical models were developed to predict grinding forces when a structured wheel was used in a grinding process. This paper aimed to establish a single grit grinding force model to predict the ploughing, friction and cutting forces in a grinding process. It took into the consideration of actual topography of the grinding wheel, a theoretical grinding force model of the grinding wheel with orderly-micro-grooves was proposed. The model was innovative in sense that it represented the random thickness of undeformed chips by a probabilistic expression, and it reflected the microstructure characteristics of the structured wheel explicitly. Note that the microstructure depended on the randomness of the protruding heights and distribution density of the grits over wheel. The proposed force prediction model was validated by surface grinding experiments, and the results showed (1) a good agreement of the predicted and measured forces and (2) a good agreement of the changes of the grinding forces along with the changes of grinding parameters in the prediction model and experiments. The proposed model was proven its accuracy, reliability and effectiveness in predicting grinding forces by an orderly-micro-grooved grinding wheel.
Regional variation of coda Q in Northwest Caucasus
Authors: Zvereva Anastasia S. , Havskov Jens , Gabsatarova Irina P. 
Published: August 2022

Abstract
Coda Q has been studied in the North-West Caucasus region using 267 earthquakes and 17 stations from the network of the Geophysical Survey, Russian Academy of Sciences. This is the first Qc study for the region using such a large dataset. The average frequency-dependent coda Q relationship is Qc=90±21∙f1.02±0.11. This value compares closely to other similar tectonic areas in the world where the same processing parameter had been used. Three specific zones, two in the Greater Caucasus tectonic area and one east of the Ciscaucasian trough area, were studied separately. There was a clear difference in Qc between the zones, particularly for the Ciscaucasian trough area which shows a higher Qc than the 2 other zones. However, the difference between the zones became smaller when using long lapse times tending to give a constant Qc as a function of lapse time. For longer lapse time it is assumed that a large part of the coda waves passes the mantle. The 2 zones in Greater Caucasus now had a similar Qc while the East zone in the Ciscaucasian trough still gave the highest values. We contribute this difference to differences in attenuation in the mantle under the two tectonic areas. In our area, there is then a clear difference in Qc for the 3 study areas in the crust but in the mantle the difference is mainly between the two tectonic zones.
Fabrication of Silver Nanoparticles-deposited Fabrics as A Potential Candidate for The Development of Reusable Facemask and Evaluation of Their Performance
Authors: Abazari Morteza , Badeleh Safa Momeni , Khaleghi Fatemeh , Saeedi Majid , Haghi Fakhri 
Published: August 2022

Abstract
Recently, wearing facemasks in public has been raised due to the coronavirus disease 2019 epidemic worldwide. However, the performance and effectiveness of many existing products have raised significant concerns among people and professionals. In this respect, doping or impregnating facemask fabrics with metallic substances or nanoparticles like silver nanoparticles has been proposed. Therefore, in the present study, we aimed to sonochemically deposit silver nanoparticles on the non-woven Spunbond substrates at different sonication times and concentrations to develop reusable antibacterial and antiviral facemask for protection against coronavirus. The coated substrates were characterized in terms of morphological, mechanical, and functional characteristics. The results confirmed high and uniform deposition of silver nanoparticles on Spunbond fabrics with desirable stability and mechanical properties. Evaluation of functional characteristics of the resultant fabrics showed suitable particle filtration efficiency, pressure drop, and electrical conductivity, which are essential elements for a desired facemask with enhanced protection and support of normal breath. The prepared fabrics also showed high antibacterial activity against E. coli and S. aureus bacteria without any significant cytotoxicity effect toward HEK 293 cells and brine shrimp.
Prognostic impact of tumor-associated macrophage-related markers in patients with adenocarcinoma of the lung
Authors: Shikanai Shunsuke , Yamada Noriyuki , Yanagawa Naoki , Sugai Mayu , Osakabe Mitsumasa , Saito Hajime , Maemondo Makoto , Sugai Tamotsu 
Published: August 2022

Abstract
Macrophage polarization is an important pathogenetic factor in neoplastic diseases and is regulated by transcription factors, i.e., phosphorylated signal transducer and activator of transcription 1 (phospho-STAT1) for the M1 phenotype and c-Maf for the M2 phenotype. However, the role of macrophage phenotype in lung adenocarcinoma (LAD) remains unclear. Here, we examined whether the density of M1 and M2 macrophages was associated with prognosis in patients with LAD using double-labeling immunohistochemistry for the detection of macrophage markers. Additionally, programmed death ligand 1 (PD-L1) expression was investigated. Immune cells co-expressing CD68 and phospho-STAT1 were considered M1 macrophages, whereas those co-expressing CD68 and c-Maf were recognized as M2 macrophages. Three hundred seven patients with LAD were divided into two cohorts (N = 100 and 207 cases, respectively) to evaluate the associations of M1 and M2 phenotypes with prognosis in patients with LAD. We determined the cut-off values of CD68/phospho-STAT1-positive cells (5 or less) and CD68/c-Maf-positive cells (more than 11) to assess correlations with survival using receiver operating characteristic curve analysis in the first cohort. According to the cut-off values, high expression of CD68/c-Maf was identified as an independent prognostic marker, whereas CD68/Phospho-STAT1 expression was inversely correlated with patient outcomes. Moreover, the M1/M2 ratio (0.19 or less) was a poor prognostic factor for LAD. However, PD-L1 expression was not correlated with patient outcomes. Overall, these findings suggested that double staining of markers identifying M1 and M2 macrophages, including phospho-STAT1 and c-Maf, can be used as prognostic indicators for patients with LAD.
Application of computed tomography-guided hook-wire localization technique in thoracoscopic surgery for small pulmonary nodules (≤10 mm)
Authors: Yang Yuan , Qin Chenhui , Ma Yue , Lu Zhongting , Zhang Yun , Li Tao 
Published: August 2022

Abstract
Objective: This study aimed to investigate the safety and efficacy of the computed tomography (CT)-guided hook-wire localization technique in thoracoscopic surgery for small pulmonary nodules (≤10 mm) and to identify the risk factors for localization-related complications.Methods: The medical records of 150 patients with small pulmonary nodules treated from January 2018 to June 2021 were retrospectively analyzed. According to preoperative hook-wire positioning status, they were divided into the localization group (50 cases) or the control group (100 cases). The operation time, intraoperative blood loss, hospital stay, and conversion rate to thoracotomy were recorded and compared between groups. Uni- and multivariate binary logistic regression analysis was used to identify the risk factors for localization-related complications.Results: A total of 58 nodules were localized in 50 patients in the localization group, and the localization success rate was 98.3% (57/58). In one case, the positioning pin fell off before wedge resection was performed. The mean nodule diameter was 7.05 mm (range, 2.8–10.0 mm), while the mean depth from the pleura was 22.40 mm (range, 5.47–79.47 mm). There were 8 cases (16%) of asymptomatic pneumothorax, 2 (4%) of intrapulmonary hemorrhage, and 1 (2%) of pleural reaction. The mean operation time of the localization group (103.88 ± 41.74 min) was significantly shorter than that of the control group (133.30 ± 45.42 min) (P < 0.05). The mean intraoperative blood loss of the localization group (44.20 ± 34.17 mL) was significantly lower than that of the control group (112.30 ± 219.90 mL) (P < 0.05). The mean hospital stay of the localization group (7.96 ± 2.34 days) was significantly shorter than that of the control group (9.21 ± 3.25 days).Multivariate binary logistic analysis showed that the localization number of small pulmonary nodules in the localization group was an independent risk factor for localization-related pneumothorax.Conclusions: Our results suggest that the CT-guided hook-wire localization technique is beneficial for localizing small pulmonary nodules. Specifically, it is helpful for the diagnosis and treatment of early lung cancer because it can accurately remove lesions, decrease intraoperative blood loss, shorten operation time and hospitalization stay, and reduce thoracotomy conversion rate. Simultaneous positioning of multiple nodules can easily lead to positioning-related pneumothorax.
Stable soil moisture altered the rhizosphere microbial community structure via affecting their host plant
Authors: Liu Dichuan , Wang Zhuan , Zhu Guolong , Zhang Renlian , Bryant Ray , Drohan Patrick J. , Long Huaiyu , Xu Aiguo 
Published: August 2022

Abstract
Temporal variation of soil moisture is one of the influencing factors affecting crop water use efficiency (WUE). Compared with fluctuating soil moisture (FSM), stable soil moisture (SSM) with weaker temporal variance has the potential to improve the WUE of crops. However, response of crop rhizosphere microbiome to soil moisture temporal variation remains unclear. In this study, we performed pot experiments on romaine lettuce (Lactuca sativa L. var. longifolia) to compare the effects of different soil moisture temporal variation on plant growth, yield, water use efficiency (WUE), and rhizosphere bacterial and fungal community structures, via manual irrigation and negative pressure irrigation to create FSM and SSM conditions, respectively. The results indicate that SSM improved the growth and WUE of romaine lettuce. Moreover, the rhizosphere microbial community composition of romaine lettuce differed under SSM and FSM conditions. Under SSM, bacterial Bacillus, fungal Aspergillus and Chaetomium were enriched in the romaine lettuce rhizosphere, whereas some taxa such as bacterial Devosia, Lysobacter, Blastococus and Bacillus, fungal Alternaria were reduced; these taxa could therefore be biomarkers in future research. Partial least squares path model (PLS-PM) analysis revealed that rhizosphere microbial communities were indirectly affected by the soil moisture temporal variation, as evidenced by the improvement in plant growth. Our results suggest that the rhizosphere microbial communities of romaine lettuce primarily respond to changes in the soil moisture temporal variation through the plant-microbiome interaction but are not directly affected by soil moisture.
Total phosphorus removal in multi-soil-layering nature-based technology: Evaluation of influencing factors and prediction using data-driven methods
Authors: Sbahi Sofyan , Mandi Laila , Ouazzani Naaila , Hejjaj Abdessamed , Lahrouni Abderrahman 
Published: August 2022

Abstract
Excess phosphorus (P) in wastewater can produce eutrophication, posing a serious risk to the safety of water resources and ecosystems. Therefore, effective pollutant removal including P from wastewater is the key strategy to save the environment and public health. Multi-soil-layering (MSL) is a promising nature-based technology that mainly relies on a soil mixture containing iron to remove P-pollution from wastewater. In the MSL influent, fourteen water quality indicators were measured, including pH, dissolved oxygen, total suspended solids, electrical conductivity, organic matter, nutrients, and coliform bacteria, to determine which ones have the strongest relationship with total phosphorus (TP) removal. The influence of hydraulic loading rate (HLR) and climatic variables (air temperature, rainfall, and evaporation) on the removal of TP was investigated. Four data-driven methods including multiple linear regression (MLR), k-nearest neighbors (KNN), random forest (RF), and neural network (NN) were conducted to predict TP removal at the MSL system outlet. In contrast to climatic variables, the results reveal that the HLR has a significant impact (p < 0.05) on TP removal (47% − 90%) in the MSL system. Furthermore, using a feature selection technique, the HLR, pH, PO43− and TP were suggested as the relevant input variables affecting TP removal in the MSL system, while an examination of accuracy shows that the RF model achieves good prediction accuracy (R2 = 0.93) and can help to understand MSL behavior for pollutants.