bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of MicroRNA-346 Exerts Positive Effects on Estrogen-Associated Molecular and Behavioral Traits in Follicular Thyroid Carcinomas
Authors: Nam Ji Sun , Nam Jung Eun , Ahn Chul Woo , Kim YuSik 
Published: November 2022

Abstract
Background
The gender disparity in the thyroid cancer incidence rate, which is 3-folds higher in women than in men, has been hypothesized to be related to estrogen and estrogens receptors (ERs). Recent evidence suggests that nuclear receptor-interacting protein 1 (NRIP1) is known as a coregulator of ERs and a direct target of microRNA-346 (miR-346), which was reported as a biomarker for follicular thyroid carcinoma (FTC). In this study, we investigated the roles of miR-346 on behavioral traits and estrogen-associated pathogenesis of FTCs.
Methods
Two Human follicular thyroid carcinomas (FTC-133 and RO82-W-1) were used. To examine the effects of miR-346 and estrogen on behavioral traits and estrogen-associated pathogenesis of FTCs, FTC-133 and RO82-W-1 were transfected with the inhibitor targeting human miR-346 or the non-specific miR (-Control), then were treated with 100nM or 200nM of estradiol-17 β (E2). Cell migration and invasion assays were performed; gene and protein levels of NRIP1, ERα, and ERβ were examined by qPCR and western blot, respectively. In addition, cell cycle-regulating genes, epithelial biomarkers, mesenchymal biomarkers were also examined.
Results
E2 decreased the number of invaded and migrated cells regardless of miR-346-downregulation in both FTCs. Downregulation of miR-346 itself also had protective effects on invasion and migration of both FTCs, and it augmented the inhibitory effects of E2. E2 decreased both gene and protein levels of NRIP1 in FTC-133 while miR-346 downregulation did in RO82-W-1. E2 and miR-346 downregulation decreased the gene and protein expressions of ERα while it increased ERβ, accordingly, decreasing the ratio of ERα to ERβ in both FTCs. E2 significantly decreased cellular proliferation in miR-346 downregulated FTCs. These support that miR-346 has significant roles in estrogen-associated pathogenesis of FTCs by regulating NRIP1 and the interaction of ERα and ERβ. Downregulation of miR-346 increased gene expressions of occluding and cloudin-1 (CLDN1) while decreasing vimentin in FTC-133, it significantly increased gene expressions of CLDN1 in RO82-W-1. These suggest miR-346 in involved in maintaining integrity of FTCs.
Conclusion
Inhibition of miR-346 in FTCs have protective effects on metastasis, thereby, miR-346 is suggested as a therapeutic target for FTC.
Integrative taxonomy for the traditional coccidians (Chromista: Miozoa: Eimeriidae) from island canaries (Aves: Passeriformes: Fringillidae): Worldwide distribution, morphological and molecular characterization, revaluations and establishment of junior synonyms
Authors: Berto Bruno Pereira , Machado Evelyn Lina , Hossotani Camila Michele de Souza , Beretta Bruna Matarucco Sampaio , Silva Débora Regina Romualdo da , Nakamura Alex Akira , Meireles Marcelo Vasconcelos 
Published: November 2022

Abstract
Island canaries Serinus canaria (Linnaeus) are finches native to the North Atlantic Islands, however, they have a worldwide distribution in captivity due to their relevance as a pet bird. Coccidians are the most reported parasites of passerines worldwide, both in the wild and in captivity, being frequently associated with disease in passerines kept in rehabilitation centers and commercial breeders. This study aimed to identify coccidians from island canaries kept in captivity in Brazil. Three hundred and fifteen genomic DNA extracted from fecal samples of island canaries from different breeders from Southern and Southeastern Brazil were used to perform a nested PCR assay to amplify a partial fragment of the 28S small subunit ribosomal RNA gene (28S) of Isospora spp. Microscopic screening and morphological identification of Isospora oocysts was performed in fecal samples corresponding to PCR positive DNA samples. Fecal samples have been formalin-stored for approximately four years. Positivity rate for both microscopy and PCR was 10.5% (33/315). Posteriorly, Isospora serini (Aragão, 1933) Box, 1975 and Isospora canaria Box, 1975 were morphologically identified from fresh fecal samples of island canaries maintained by a breeder in the State of São Paulo, Southeastern Brazil, providing a genotypic characterization via sequencing of the mitochondrial cytochrome c oxidase subunit 1 (COI) and 28S genes. The 28S and COI sequences referring to the morphological identification of I. canaria was, respectively, 100% and 99% similar to sequences deposited as Isospora serinuse Yang, Brice, Elliot & Ryan, 2015 from island canaries kept in a rehabilitation center in Australia. The COI sequence referring to the morphological identification of I. serini was 100% similar to a sequence of an extraintestinal Isospora, corroborating this identification/sequencing since I. serini is the first isosporan with an extra-intestinal cycle demonstrated. The comparison of morphological and molecular data from I. canaria and I. serini from this study with published data of Isospora spp. from canaries worldwide, allowed the specific identification from preliminary generic identifications, correction of misidentifications, as well as the establishment of junior synonyms. Finally, this study provides morphological and molecular data that ensure the correct identification of the two Isospora spp. from island canaries in future studies worldwide.
CORREL: Automated Onset Estimation for Controlled-Source Seismic Experiments
Authors: Reyes-Wagner Valentina , Comte Diana , Roecker Steven W. , Rietbrock Andreas 
Published: November 2022

Abstract
Estimates of the onset times of P phases from active source experiments can effectively be used in developing wavespeed models, and the large number of recordings typical of such experiments incentivizes the development of automated approaches to generate these estimates. The simplicity and repeatability of an airgun source such as that used in the 2016 Pisagua/Iquique Crustal Tomography to Understand the Region of the Earthquake Source (PICTURES) project in northern Chile suggested that a straightforward application of waveform cross-correlation would suffice for arrivals recorded by a network of inland seismic stations, but did not work well due to significant variations in waveform morphology. Application of an alternative algorithm typically used in passive source investigations, the Regressive ESTimator (REST) autopicking package, also proved unsatisfactory, largely because the limited spectral bandwidth of the airgun source and the frequent occurrence of local seismicity led to numerous false picks. This motivated the development of a new approach, named CORREL, that is a hybrid of REST and cross-correlation, with the addition of a constraint in the form of a polynomial function based on the REST picks that provides a reasonable prediction of an onset time. Compared to the results obtained originally from REST and simple waveform correlation, the application of CORREL to the PICTURES data both significantly increased the number of arrivals detected and greatly reduced the number of outliers. The predictive polynomial also provides CORREL a better means to discriminate true shots from the abundant natural seismicity.
NF-κB pathway related long non-coding RNAs in inflammatory bowel disease
Authors: Kazemifad Nesa , Kazemi Mohammad , Shahrokh Shabnam , Aghdaei Hamid Asadzadeh 
Published: November 2022

Abstract
Background: Inflammatory bowel disease (IBD) is a complex disease classified into two types, ulcerative colitis (UC) and Crohn’s disease (CD), and it results from the interaction between genetic and environmental factors influencing immune responses. However, the etiology of the IBD has not yet been fully understood. The nuclear factor kappa beta (NF-κB) pathway is one of the major regulators of inflammatory responses associated with the pathogenesis of IBD. The up-regulation in NF-κB expression in mucosal macrophages and monocytes induces increased production of pro-inflammatory cytokines. Several recent studies have indicated abnormal function and expression levels of non-coding RNAs, including lncRNAs, in the pathogenesis of IBD. Various lncRNAs, involved in immune responses were suggested to associate with IBD pathogenesis and maintenance of intestinal mucosa function via the interaction network between miRNAs, transcription factors, and mRNAs.
Method: In this study, we aimed to predict regulatory lncRNAs for the NF-κB pathway with bioinformatics studies and validate the expression of 4 predicted lncRNAs (NRAV, HULC, RAD51-AS1, and SBF2-AS1) in blood and tissue samples of UC patients.
Results: The qPCR results indicated that NRAV and HULC are upregulated in UC colonic samples, and RAD51-AS1 and SBF2-AS1 are upregulated in blood samples of UC patients compared to controls.
Conclusion: This study indicated that bioinformatics studies could successfully predict disease-related lncRNAs and studied lncRNAs such as HULC, NRAV, RAD51-AS1 and SBF2-AS1 may have and important role in controlling inflammation in IBD.
Comparison of transcatheter arterial chemoembolization versus systemic chemotherapy for elderly patients with advanced gastric cardiac cancer
Authors: Li Zhenfeng , Xu Ran , Yan Zaojun , Chang Xu , Zhang Jianxin , Liu Zihao , Wu Huiyong , Sun Peng 
Published: November 2022

Abstract
Purpose
To compare the efficacy, safety and effects on health-related quality of life (HRQOL), nutritional status and remission of dysphagia of transcatheter arterial chemoembolization (TACE) with that of systemic chemotherapy for elderly patients with advanced gastric cardiac cancer (AGCC).
Methods
117 elderly patients(age > 70 years old) with AGCC were retrospectively enrolled. 56 patients received TACE and 61 patients received systemic chemotherapy. Overall survival (OS) and frequency of adverse events (AEs) were observed. HRQOL scores, nutritional status based on scored patient-generated subjective global assessment (PG-SGA) and the degree of dysphagia at baseline,4 weeks and 8weeks were compared between groups.
Results
No significantly difference was found in baseline characteristics between groups, except the age (75 vs 73, p < 0.05). The OS time was not significantly different between groups (11.75 months vs 12.73 months, P = 0.916). The HRQOL scores at 4 weeks and 8 weeks after treatment in TACE group were significantly higher than that in systemic chemotherapy group (12.0 vs 9.0 ,18.0 vs 12.0, P < 0.001). After 4 weeks and 8 weeks of treatment, the nutritional status of patients in the TACE group was significantly better than that in the systemic chemotherapy group(P < 0.05). The average degree of dysphagia in the TACE group were significantly lower than that in the systemic chemotherapy group at 4 weeks and 8 weeks (1.5 vs 2.3,1.2 vs 1.6, P = 0.000). AEs were more frequent in the systemic chemotherapy group than in the TACE group(P < 0.05).
Conclusion
Compared with systemic chemotherapy, TACE can more effectively alleviate dysphagia and improve HRQOL and nutritional status in elderly patients with AGCC.
Lapatinib dysregulates HER2 signaling and impairs the viability of human uveal melanoma cells
Authors: Shu Wenying , Wang Janney Z , Zhu Xue , Wang Ke , Cherepanoff Svetlana , Conway R. Max , Madigan Michele C , Lim Li-Anne , Zhu Hong , Zhu Ling , Murray Michael , Zhou Fanfan 
Published: November 2022

Abstract
Although uveal melanoma (UM) is rare, it is the principal type of intraocular malignancy. Up to 50% of UM patients develop metastatic disease with low chances of survival beyond 18 months. At present there are no drugs that are effective in the treatment of primary or metastatic disease. We recently tested the hypothesis that the ErbB receptor family member HER2 may be a novel drug target in UM. We found that afatinib, which targets several ErbB receptors, including HER2, has potent anti-cancer and anti-metastatic actions in UM. The present study was undertaken to further evaluate the potential value of HER2 targeting in UM using the multi-kinase inhibitor lapatinib, that is currently approved for the treatment of HER2-positive cancers in patients.
The anti-UM actions of lapatinib were assessed using cell viability, cell death and cell cycle analysis, and its anti-metastatic actions were evaluated using would healing and colony formation assays. Immunoblotting was used to substantiate the actions of lapatinib on apoptotic and HER2 signaling. The anti-UM actions of lapatinib were further evaluated in vivo in a UM xenograft mouse model.
Lapatinib decreased the viability of four UM cell lines (IC50 values in MTT reduction assays were 3.67-6.53 µM). The antiproliferative activity of lapatinib was corroborated in UM cells isolated from patient tumors. In UM cell lines lapatinib promoted apoptosis and cell cycle arrest, and strongly inhibited cell migration and reproductive cell growth. Lapatinib dysregulated HER2-AKT/ERK/PI3K signalling in UM cell lines, as indicated by increased STAT1 expression, decreased expression of BCL-xL and cyclin D1, and decreased Bcl-xL:Bax ratio, which is consistent with enhanced apoptosis. Importantly, lapatinib and suppressed tumourigenesis in vivo in mice carrying UM cell xenografts.
Together the present findings are consistent with the assertion that HER2 is a viable therapeutic target in UM. Lapatinib was active in primary and metastatic UM and is a clinically approved HER2 inhibitor. The activity of lapatinib in UM patients could now be evaluated directly in clinical trials.
High-Throughput Quantitative Proteomic Analysis of Coxsackievirus A16-infected 16HBE Cells Based on tandem mass tag (TMT)-labeled Quantitative Proteomics Running title: Proteome profiling of Coxsackievirus A16 in 16HBE cells
Authors: Hu Yajie , Liu Chen , Zhong Mingmei , Qian Baojiang , Qiu Jingman , Chen Juan , Zhang Yunhui , Song Jie 
Published: November 2022

Abstract

Hand, foot and mouth disease (HFMD) caused by Coxsackievirus A16 (CV-A16) is a global health concern worldwide. There are no vaccines or antiviral compounds available to either prevent or treat CV-A16 infection which may trigger severe neurological complications, and even lead to death. Moreover, its pathogenic mechanisms and pathophysiology are still poorly elucidated. To increase our understanding of the interaction of CV-A16 with the host cell, we analyzed changes in the proteome of 16HBE cells in response to CV-A16 infection using tandem mass tag (TMT) in combination with LC-MS/MS. It was identified and quantified 6615 proteins and there were 172 proteins showed a significant alteration during CV-A16 infection. To validate the proteomics data, 3 randomly selected proteins exhibited consistent changes in protein expression with the TMT results using a Western blotting and immunofluorescence method. Then, functional enrichment analysis showed that these differentially expressed proteins mainly involved in various biological processes and signaling pathways, such as metabolic process, Cytokine-cytokine receptor interaction, B cell receptor signaling pathway, Neuroactive ligand-receptor interaction, etc. And further bioinformatics analysis revealed that these differentially expressed proteins contained distinct domains, localized in different subcellular components, and established a complex network. In conclusion, results from this study have helped elucidate the molecular pathogenesis of CV-A16 and may facilitate the development of new antiviral therapies as well as innovative diagnostic methods.
Molecular detection of ESBL- and carbapenemase-producing Klebsiella pneumoniae isolates in southwest Iran
Authors: Jomehzadeh Nabi , Rahimzadeh Mohammad , Ahmadi Bahare 
Published: November 2022

Abstract
Background: Carbapenem-resistant clinical isolates are extending rapidly, and in past recent years, carbapenem resistance has become an important health problem worldwide. This study aimed to investigate the carbapenem-resistance genes in extended-spectrum β-lactamase (ESBL) producing K. pneumoniae isolates.
Methods: Seventy-five non-duplicate clinical K. pneumoniae strains were isolated from urine, blood, sputum, and wound samples. Antimicrobial susceptibility tests for 12 different antibiotics were performed using the disk diffusion method, followed by the determination of minimum inhibitory concentrations (MIC) of imipenem and meropenem. Phenotypic detection of ESBL and carbapenemase enzymes was performed by disk Disc Diffusion Synergy test (DDST) and modified Hodge test (MHT), respectively. Resistant isolates were further investigated for ESBL and carbapenemase genes by the PCR assay.
Results: The highest and lowest resistance rates were observed against ampicillin (93.3%) and tigecycline (9.3%), respectively. Based on the results of phenotypic tests, 46.7% and 25.3% were positive for ESBL and carbapenemase enzymes, respectively. In addition, using the molecular method, the predominant ESBL-, and carbapenemase-associated genes were blaTEM (34.3%) and blaOXA-48 (57.8%), respectively.
Conclusion: Based on the study, it has been found that K. pneumoniae strains produce significant rates of beta-lactamase enzymes, which is extremely alarming. As carbapenem resistance is an alarming public health issue, early detection of the isolates and effective infection control measures are necessary to prevent their further spreading.
Changes in psychosocial distress and the number and types of problems reported by patients with cancer when routine screening is integrated within cancer services
Authors: Faris Mona M , Shepherd Heather L , Butow Phyllis N , Kelly Patrick , He Sharon , Grimison Peter , Kelly Brian , Group The ADAPT Program , Shaw Joanne 
Published: November 2022

Abstract
Purpose
The impact of measuring patient-reported outcomes on longitudinal clinical outcomes is not well understood. This study explored longitudinal changes in distress and problems reported by cancer patients screened and managed in accordance with a clinical pathway for anxiety and depression (ADAPT CP), implemented over 12 months.
Methods
Patients reported distress using the Distress Thermometer, and indicated reasons for distress using the 39-item Problem List across five domains: practical, social, emotional, spiritual/religious and physical. Repeat screening occurred on average 3-monthly.
Results
660 patients from 10 participating services completed 1,256 screening events over 12 months, reporting 8,645 problems. On average, more emotional (27–34%) and physical (19–22%) issues were reported across all quarters than practical (7–9%) and social (8–9%). Reporting of distress and emotional, physical, practical and social problems reduced from initial to follow-up screens. Younger age predicted increased distress and emotional, practical, and social problems. Longer time since diagnosis (> 6 months) predicted decreased distress and physical problems. Worry, fatigue, sleep difficulties, health of family members, and insurance/finances were more persistent problems.
Conclusion
Routine anxiety/depression screening is recommended within oncology settings to allow patients to indicate new or persistent problems and hospital staff to monitor and assess needs. Emotional concerns are high in oncology patients, suggesting prioritisation of psychosocial care. These problems can persist over time suggesting the challenge to address these in clinical care, or that access to, or implementation of, evidence-based interventions are not yet widespread.
Trial registration
This study was part of a larger program of work was registered prospectively with the ANZCTR on March 22, 2017. Trial ID ACTRN12617000411347
The Clinical Value of Lower Gastrointestinal Symptoms for Colorectal Polyps: A Retrospective Study of Colorectal Polyps and Symptoms
Authors: Kuai Da-Yu , Song Yu-Li , Li Ya-Qiang , Sun Wei , Hu Yan-Ping , Min Li , Xu Bao-Hong 
Published: November 2022

Abstract
Purpose: To analyse the relationship between lower gastrointestinal symptoms and clinicopathological features in colorectal polyp patients.
Methods: We retrospectively collected the clinicopathological information of outpatients undergoing colonoscopy atLuhe Hospital Affiliated with Capital Medical University.
We performed tendency score matching (PSM) between colorectal polyp patients and polyp-free patients by age and sexat a ratio of 1:1 andthen analysed the relationships between colorectal polyps and clinical symptoms by univariate and multivariate analyses.
Results: 1) This study included 2483 patients with colorectal polyps and 2483 polyp-free patients. 2) The proportion of abdominal distension and haematochezia was increased in patients with colorectal polyps (25.6% vs. 22.9%, P = 0.024; 21.5% vs. 16.8%, P = 3.4E-5), but the proportion of diarrhoea wasdecreased (35.1% vs. 37.9%, P = 0.036). 3) Patients with haematochezia usually hadlarger polyp sizes [univariate p= 0.014; multivariate 95% CI1.034 (1.001, 1.069), p = 0.043]. 4) Patients with diarrhoea typically had a smaller polyp size [univariate p = 0.078; multivariate 95% CI0.971(0.944, 0.999), p = 0.043]. 5) Constipation and other symptoms had little correlation with the clinicopathological features of colorectal polyps.
Conclusions: Haematochezia is positively correlated with the presence and size of colorectal polyps, and diarrhoea is negatively correlated with the presence and size of colorectal polyps.
On Convergence of the Uniform Norm and Approximation for Stochastic Processes from the Space Fψ(Ω)
Authors: Rozora Iryna , Mlavets Yurii , Vasylyk Olga , Polishchuk Volodymyr 
Published: November 2022

Abstract
In the paper, we consider random variables and stochastic processes from the space Fψ(Ω) and study approximation problems for such processes. The method of series decomposition of stochastic processes from Fψ(Ω) is used to find an approximating process called a model. The rate ofconvergence of the model to the process in the uniform norm is investigated. We develop an approach for estimating the cutting-offlevel of the model under the given accuracy and reliability of the simulation.
MSC Classification: 60G07, 60G15, 65C20, 68U20
Assessment of Depression and Anxiety in Young and Old with a Question Based Computational Language Approach
Authors: Sikström Sverker , Kelmend Bleona , Persson Ninni 
Published: November 2022

Abstract
Older adults experience depression and anxiety differently than younger adults. Age may affect circumstances, depending on accessibility of social connections, jobs, physical health, etc, as these factors influence the prevalence and symptomatology. Depression and anxiety are typically measured using rating scales, however, recent research suggests that such symptoms can be assessed by open-ended questions that are analysed by question-based computational language assessments (QCLA). Here, we study older and younger adults’ responses about their mental health using open-ended questions and rating scales about their mental health. We then analyse their responses with computational methods based on natural language processing (NLP). The results demonstrate that: (1) older adults describe their mental health differently compared to younger adults; (2) where, for example, older adults emphasise depression and loneliness whereas young adults list anxiety and money; (3) different semantic models are warranted for younger and older adults; (4) compared to young participants, the older participants described their mental health more accurately with words; (5) older adults have better mental health than younger adults as measured by semantic measures. In conclusion, NLP combined with machine learning methods may provide new opportunities to identify, model, and describe mental health in older and younger adults. These semantic measures may provide ecological validity and aid the assessment of mental health.