bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of a serum multi-omics based diagnostic model for systemic lupus erythematosus
Authors: Wang Wei , Wang Yingzhuo , Guo Feng , Yu Qian , He Qiongzi , Wei Peng , Wang Xiaoxiao , Liu Shijia 
Published: August 2022

Abstract
Objective.
The study aimed to identify potential biomarkers (metabonomics and proteomics) of systemic lupus erythematosus (SLE), and develop serum multi-omics based diagnostic models for SLE.
Methods.
Serum samples were collected from 111 SLE patients and 38 healthy controls (HCs). Gas chromatography-mass spectrometry (GC-MS) and Tandem Mass Tag (TMT) quantitative detection technology were respectively used to evaluate the subjects' serum metabolic spectrum and protein. Least absolute shrinkage and selection operator (LASSO) regression and stepwise logistic regression were used to select clinical characteristics, metabonomics and proteomics parameters associated with SLE and the severity of SLE. Three diagnosis models were developed and validated to diagnosis SLE and the severity of SLE. The receiver operating characteristic curve (ROC), sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were utilized to evaluated three diagnostic models. Three nomogram were plotted to visualize the three models.
Results.
12 variables of all variables (2 demographic characteristics, 9 laboratory indexes, 8 proteomic indexes and 339 metabolomic indexes) were selected, which can be used for the diagnosis of patients with different severity of SLE. Area under ROC (AUC) of three models were 0.932, 0.840 and 0.867, respectively. Meanwhile, the sensitivity and specificity of three models were high (Mode l: 0.956 and0.857, Model 2: 0.700 and 0.900, Model 3: 1.000 and 0.667).
Conclusion.
The changes of serum metabolites and proteins in patients with SLE were studied by multi-omics analysis, and three multi-omics based diagnosis models and nomograms for were developed and validated to diagnosis SLE and the severity of SLE.
Negative Effects of Cx43 during Denervated Skeletal Muscle Atrophy can be Attenuated by Blueberry Extracts via Regulating Akt Pathway
Authors: Huang Xinying , Zhang Junpeng , You Zongqi , Xiang Yaoxian , Dai Junxi , Xu Lei , Jiang Junjian , Liu Jibo , Xu Jianguang 
Published: August 2022

Abstract
Background: Lack of effective treatment for alleviating the irreversible skeletal muscle atrophy caused by peripheral nerve disfunction has long been a clinical problem. For seeking therapeutic targets, several signaling pathways in muscle cells that activated by denervation have been explored. Important roles of Cx43 in other types of pathologic processes such as have been reported. Methods: Denervation mice model was created. Hematoxylin–Eosin (HE) and immunofluorescence staining were applied and analyzed. Western blot and quantitative real-time PCR (qPCR) were used. All statistical testing was performed by t test.Results: Our study investigated the negative role of Cx43 during muscle cells degeneration after denervation. Blueberry extracts was applied orally and its protective effect for denervated skeletal muscle atrophy was found in our study, and we demonstrated that Cx43/Akt pathway in denervated muscle cells was modulated by blueberry extracts. Conclusion: Negative effects of Cx43 during denervated skeletal muscle atrophy can be attenuated by blueberry extracts via regulating Akt pathway.
Are public good ecosystem services generated by on farm conservation of crop diversity valued by the general public?
Authors: Drucker Adam G. , Pradel Willy Willy , Scott Craig , Elmes Sarah , Valero Kleny G. Arpazi , Zander Kerstin K. 
Published: August 2022

Abstract
Agrobiodiversity is associated with a range of important but poorly quantified public good ecosystem services, the conservation of which requires public support. With a view to determining the general public’s willingness to pay (WTP) for such conservation, 491 adult Peruvian residents were interviewed using a stated preference choice experiment (CE) to elicit the value they place on crop genetic resources conservation, using quinoa as a case study. Strong support for the conservation of quinoa diversity was revealed, in particular when conservation was framed in terms of conserving the national cultural identity or food security. Respondents were willing to donate most for quinoa conservation to minimise production losses and to maintain the crop’s existence. Findings demonstrate the significant and frequently ignored social welfare benefits associated with non-market agrobiodiversity-related public good ecosystem services, in this case equivalent to a third of market production values. Given the relatively modest costs of securing such ecosystem services, we calculated a conservative benefit-cost ratio of 1.7.
Capital Accumulation in the Informal Sector: A Bibliometric Analysis
Authors: Rajput Dinesh , Sreera Binilkumar Amarayil 
Published: August 2022

Abstract
The role of capital accumulation in economic growth is widely discussed. The role of the informal sector in the economic growth of many emerging and least developed economies is also widely discussed. This paper explores the role of capital accumulation in the informal sector. The informal sector enterprises face problems in accessing capital and funding their day-to-day operations. Hence it becomes important to understand how these firms expand their capacity. This paper evaluates the existing literature on capital accumulation in the informal sector with the help of bibliometric analysis. A bibliometric analysis is a quantitative analysis of bibliometric data that helps in understanding the trends in any research topic. The bibliometric tools have helped us analyse the theoretical framework, research collaboration, country-wise contribution, thematic evolution of research themes, and emerging areas for research in capital accumulation in the informal sector. In the conclusion, we reconfirm the growing popularity of capital accumulation and the informal sector and the role of capital accumulation in the growth of informal enterprises. The study suggests future research areas such as the role of social capital, intellectual capital, and human capital in capital accumulation and its impact on the growth of the informal sector. Plain English Summary Entrepreneurs and small informal firms crave social, intellectual, and human capital. This is a bibliometric study of research papers published in capital accumulation in the informal sector domain in the last 30 years. The study focuses on the theoretical evolution of capital accumulation and informal sector concepts. It has used bibliometric data of well scrutinized 284 documents for statistical analysis to find out the most relevant publications, authors, sources, and annual productivity in the said domain. The study has also looked at the thematic evolution of research topics in this area over the last three decades. The findings of the study suggest that though capital accumulation has been an important element in the growth of firms its role in the informal sector is yet to be established. However, social capital, knowledge, and human capital are emerging topics that need to be explored further. The study has explored the probable research topics and emerging research areas in capital accumulation in the informal sector domain.
Assessment of air quality before and during the COVID-19 and its potential health impacts in an arid oasis city: Urumqi, China
Authors: Sun Yaxin , Aishan Tayierjiang , Halik Ümüt , Betz Florian , Rezhake Remila 
Published: August 2022

Abstract
Air pollution has become one of the major environmental concerns to academia and urban residents. As a key megacity city of the "Silk Road Economic Belt", Urumqi has been listed as one of the ten most polluted cities in the world, posing a serious threat to the urban environment and residents' health. Therefore, the assessment of air pollution and its health impact on urban residents is urgently required for promoting the sustainable development of the city. This study analyzed the temporal and spatial changes in the concentration of major air pollutants in Urumqi and its response to epidemic prevention and control measures based on the data of PM2.5, PM10, SO2, NO2, CO, and O3_8h from 10 air quality monitoring stations in Urumqi from January 1, 2017, to December 31, 2021, mainly focusing on the temporal and spatial variation characteristics of PM2.5 and O3_8h concentrations, changes in pollution levels and their potential risks on human health. The results showed that the concentrations of air pollutants PM2.5, PM10, SO2, NO2, CO, and O3_8h at various air quality monitoring sites from 2017 to 2021 showed a cyclical trend, and the implementation of the government's strict COVID-19 prevention and control measures had a significant effect on the reduction of air pollutant concentrations. The mean value of ρ(PM2.5) decreased from 2017 to 2021, while ρ(O3_8h) showed a waveform change trend (increased in 2017–2018, decreased in 2018–2020, and increased after 2020 ). In terms of the distribution area, the variation of ρ(PM2.5) and ρ(O3_8h) were greater in industrial areas than in commercial and residential areas. Continuous wavelet coherence exists between AQI and PM2.5 and O3_8h at each site during the period from January 2018 to December 2020, and AQI and PM2.5 were positively correlated. The high pollution levels (grades IV and V) of air pollutants PM2.5 and O3_8h in Urumqi decreased year by year, indicating that the number of days with potential threats to residents' health is gradually decreasing. Therefore, more attention should be paid to the control and governance of air pollution in Urumqi.
Use of trans-complementation method to determine the effects of various ftsI mutations on β-lactamase negative ampicillin resistant (BLNAR) Haemophilus influenzae strains
Authors: YALÇIN Melis , TRISTRAM Stephen , BOZDOĞAN Bülent 
Published: August 2022

Abstract
Haemophilus influenzae is causative agent of serious infections especially among children. β-lactam antibiotics are commonly used for treatment of these infections. Among H. influenzae isolates, β-lactam resistance can be due to the presence of either a β-lactamase, or in β-lactamase negative ampicillin resistant strains (BLNAR), to mutations in the ftsI gene that generate altered PBP3 with reduced affinity for β-lactams. The role of specific amino acid substitutions in PBP3 in producing a BLNAR phenotype has been thus far demonstrated by ftsI sequencing and deduced PBP3 amino acid substitutions of large numbers of strains with a BLNAR phenotype, by transforming susceptible recipient strains with ftsI genes from BLNAR strains, and by using site directed mutagenesis of the ftsI gene to generate desired substitutions. However, reversal of resistance in BLNAR strains by trans-complementation with wild-type ftsI has never been demonstrated and might be a useful tool in the investigation of β-lactam resistance in H. influenzae.The aim of this study was to determine the effect of trans-complementation with wild-type ftsI on the β-lactam susceptibility of H. influenzae with various β-lactam susceptibility phenotypes and genotypes. For this purpose, wild type ftsI gene encoding for PBP3 was amplified in whole from β-lactam susceptible H. influenzae Rd and cloned in pLS88 plasmid to obtain pADUTAS17, which was then used to transform known BLNAR strains, susceptible strains, and a strain (CF55) with wild type ftsI but unexplained reduced β-lactam susceptibility. Ampicillin and cefotaxime MICs were determined after transformation with pLS88 and pADUTAS17 plasmids. The results showed that antibiotic susceptibilities were not affected by trans-complementation for isolates carrying wild type ftsI gene, including strain CF55. However, trans-complementation for all BLNAR strains showed decreases between -0.957 and 0.5-fold for ampicillin and cefotaxime, confirming the role of the PBP3 substitutions in the BLNAR phenotype of these isolates. These results show that trans-complementation might be a useful tool in the investigation of decreased β-lactam susceptibility in H. influenzae.
Ligand free, copper catalyzed coupling reaction of aryl aluminum reagents with aryl thiosuccinimides
Authors: Xiao Hongliu , Zhang Zhihao , Jia Xiaoying , Pu Jiaxia , Wu Chuan , Li Qinghan 
Published: August 2022

Abstract
Highly efficient and simple cross-coupling reactions of aryl aluminum reagents with aryl thiosuccinimides for the synthesis of diarylsulide derivatives using a CuCl (20 mol%) as catalyst are reported. The method avoids the use of expensive transition metals like Pd, Ir or Rh, phosphorus ligand. 33–68% isolated yields of diarylsulides were obtained for a wide range of substrates at 60oC for 1.5h in THF.
ARF6-AMAP1 pathway induces pancreatic cancer cells to express immunosuppressive chemokines
Authors: Hashimoto Ari , Handa Haruka , Hata Soichiro , Okuzaki Daisuke , Fumoto Yoshizuki , Kuroda Kanon , Tsutaho Akio , Nishikawa Yoshihiro , Kodama Yuzo , Hirano Satoshi , Hashimoto Shigeru , Sabe Hisataka 
Published: August 2022

Abstract
Aim: The tumor microenvironment (TME) of pancreatic ductal adenocarcinoma (PDAC) is highly immune suppressive with modest T-cell infiltration, and only rarely responds to immune checkpoint inhibition (ICI) therapy. Our aim was to clarify the molecular basis underlying the immune suppressive properties of PDAC.Background: Mutations in both KRAS and TP53 genes are characteristic of PDAC. A major target of KRAS/TP53 double mutations is the ARF6-AMAP1 pathway, which promotes the fibrosis, acidosis, invasion, and immune evasion of cancer cells. KPC cells are a genetically well-defined mouse cell model of human PDAC bearing Kras/Trp53 mutations, and express Arf6 and Amap1 at high levels.Findings: Intact KPC cells predominantly expressed immune suppressive chemokines, such as Cxcl12 and Cxcl5, but when Amap1 was silenced, immune surveillance chemokines, such as Cxcl10 and Ccl5 became predominant. A similar, albeit fragmented, chemokine switching was observed in human PDAC cells, and this mechanism appeared to be used in the normal pancreas of humans and mice. The silencing of Amap1 in KPC cells significantly changed the types and numbers of tumor infiltrating lymphocytes to be less favorable for immune evasion.Conclusions: Many PDAC cells appear to have a hitherto unidentified mechanism of malignancy, by which they can change the types of chemokines that they produce to be favorable for immune evasion. The ARF6-AMAP1 pathway is integral to this chemokine switching. Hence, this malignancy appears to be the result of KRAS/TP53 double mutations, which are not common in other types of cancer unless they are in the advanced stages. Our results are consistent with our previous findings that blockade of the ARF6- AMAP1 pathway significantly improves the efficacy of ICI therapy.
“It’s too hard” – the management of latent TB in under-served populations in the UK: a qualitative study
Authors: Gray Adam Thorburn , Surey Julian , Esmail Hanif , Story Alistair , Harris Magdalena 
Published: August 2022

Abstract
Backgr﻿oundUK national guidance recommends systematic screening for latent tuberculosis infection (LTBI) in under-served populations, including people experiencing homelessness and people who use drugs. This is not routinely implemented in the UK, and the reasons for this policy-practice mismatch remain underexplored.MethodsSemi-structured qualitative interviews were conducted with 19 healthcare professionals from across the UK. Participants were recruited using purposive sampling and snowballing, identifying individuals with excellent knowledge of their regions practice and policy of LTBI management. The interviews were conducted online, and were audio recorded, with transcripts thematically analysed using a two-stage inductive coding process to explore perceived barriers and enablers to LTBI screening.ResultsMost participants had previous experience managing LTBI in under-served populations, but none were conducting systematic screening as per national guidance. We identified service provision challenges and low prioritisation of LTBI as the key explanatory themes driving this policy-practice mismatch. Lack of resource, and the complexity of clinical decision making were two key service level barriers. System and service inertia, and lack of cost effectiveness evidence led to LTBI being deprioritised. Service integration and promotion of WHO targets for TB elimination were highlighted as potential solutions.ConclusionIntegrating LTBI testing and treatment with existing health services for under-served populations could improve feasibility and efficacy. Promotion of UK TB elimination goals and generation of regional evidence to support commissioning for LTBI care is vital. Without such a multi-pronged approach inertia is likely to persist and the zeitgeist will remain: “it’s too hard”.
Effects of Tourism Concessions in Protected Areas around the world
Authors: Gomes Carolina Ribeiro , Junior Wanderley Jorge da Silveira , de Souza Cleber Rodrigo , Salvio Geraldo Majela Moraes , de Moura Aloysio Souza , Silva André Luiz Ferreira da , Rodrigues Carolina Costa , Machado Felipe Santana , Lana Gabriela Furbino Brêttas , Costa Lauana Silva da , Alves Lívia Laiane Barbosa , Fontes Marco Aurélio Leite 
Published: August 2022

Abstract
This study aimed to investigate tourist concessions around the world and their main effects. To do so, we explored the descriptive information according to specific variables and the existing balance between positive and negative effects. We found that the concessions fulfill their economic role through the development of tourist activity, but they have deficiencies in social, environmental and procedural parameters. The most significant positive effect especially concerns the economy and tourism. On the other hand, the negative social effect showed a higher percentage and is related to the lack of partnership with the community. Most concession types are strictly linked to tourism by large companies. The local population is not fully included in the processes or considered partners, and they only participate in the provision of services. We also found an imbalance between positive and negative effects, with evidence of inconsistency in the processes. The processes considered inconsistent compromise local sustainability, the management objectives of the areas, and generate unequal opportunities. This scenario reveals that tourism concessions are still fragile and can compromise sustainable tourism development and nature conservation in PA. Furthermore, processes based on economic policies when implemented in conservation efforts only guarantee the maximization of economic benefits, disfavoring social and environmental objectives. We defend the control and continuous monitoring of concessions in order to maintain more consistent and effective processes.
Hydrogen-oxygen therapy improves postoperative pulmonary functions and accelerates recovery through attenuating inflammatory reactions and oxidative stress in patients undergoing lung surgery
Authors: Yang Wan-chao , Zhang Yu-Rong , Yu Jia-Xing , Yang Yi , Liu Xiang-Nan , Zhang Xin , Zhai Jia-Yuan , Lai Pei-Chen , Wang Qiu-Shi 
Published: August 2022

Abstract
Purpose: To investigate whether hydrogen-oxygen (67%–33%) therapy could improve the postoperative pulmonary function, recovery, complications, inflammatory reactions, and oxidative stress in patients undergoing lung surgery.Methods: We performed a prospective, randomized, single-blind, controlled clinical trial between December 2020 and December 2021. Eligible patients received either a lobectomy or sublobar resection. Postoperatively, they had 2-hour hydrogen-oxygen therapy (group H) or oxygen therapy (group C) daily, for three consecutive days. The pulmonary functions, recovery, pain intensity, oxygen saturation, inflammatory cytokines, and anti-oxidative markers were documented.Results: A total of 66 patients were analyzed, with 33 patients in each group. Both groups had decreased postoperative pulmonary functions, but group H had significantly better postoperative pulmonary functions than group C (forced vital capacities were 1.4±0.5 and 1.0±0.4 L and forced expiratory volumes in the first second were 1.1±0.3 and 0.9±0.3 L in group H and C, respectively (both P<0.01)). Compared to group C, group H had significantly decreased drainage volume, reduced catheter indwelling duration, shortened length of hospital stay, and lower pain intensity. Group H had statistically significantly decreased postoperative serum cytokine TNF-α and IL-6 levels and increased anti-oxidative markers compared to group C. The incidences of postoperative complications were comparable between the two groups. Conclusion: Hydrogen-oxygen therapy could improve postoperative pulmonary functions, decrease pain intensity, accelerate recovery, and shorten the length of the hospital stay in patients who have undergone lung resection, which may be related to its anti-inflammatory and anti-oxidative effects. Trial registration: The Chinese Clinical Trial Registry (registration number ChiCTR2100042101)，the date of registration was January,13,2021.
Long-term bifurcation and stochastic optimal control of a triple-delayed Ebola illness model with vaccination and quarantine strategies
Authors: Din Anwarud , Sabbar Yassine 
Published: August 2022

Abstract
Discovered in 1976, Ebola illness (EI) has long been neglected despite its high mortality rate, which can be as high as 90%. For more than 40 years, this illness has been responsible for epidemics throughout Central Africa. But only recently, during the 2014-2015 epidemic in West Africa, which is the deadliest to date, has the entire world considered this disease as one of the major public health issues. This paper aims to explore the effect of external fluctuations on the prevalence of EI. We begin by proposing a sophisticated biological system that takes into account vaccination and quarantine strategies as well as the effect of time lags. Due to some external perturbations, we extend our model to the probabilistic formulation with white noises. The perturbed model takes the form of a system of stochastic differential equations. Based on some non-standard analytical techniques, we demonstrate two main approach properties: intensity and elimination of EI. In order to better understand the impact of applied strategies, we deal with the stochastic control optimization approach by using some advanced theories. All this theoretical arsenal has been numerically confirmed by employing some real statistical data of EI. Finally, we mention that this work could be a rich basis for further investigations aimed at understanding the complexity of EI propagation at a pathophysiological and mathematics levels.