bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and characterisation of Klebsiella pneumoniae and Pseudomonas aeruginosa clinical isolates with atypical β-lactam susceptibility profiles using Orbitrap liquid chromatography-tandem mass spectrometry
Authors: Takebayashi Yuiko , Dulyayangkul Punyawee , Satapoomin Naphat , Wan Nur Ismah Wan Ahmad Kamil , Williams O. Martin , Macgowan Alasdair P. , Heesom Kate J. , Williams Philip B. , Avison Matthew B. 
Published: February 2022

AbstractThere is significant interest in the possibility of predicting antibacterial drug susceptibility directly though the analysis of bacterial DNA or protein. We report the use of Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii transformants to define baseline predictive rules for the β- lactam susceptibility profiles of β-lactamase positive clinical isolates. We then deployed a robust and reproducible shotgun proteomics methodology to identify β-lactamase positivity and predict β-lactam susceptibility by reference to our baseline predictive rules both in cultured bacteria and in extracts of culture-positive blood. Proteomics and whole genome sequencing then allowed us to characterise K. pneumoniae and P. aeruginosa isolates that differed from the expected β-lactam susceptibility profile, iteratively expanding our predictive rules. Proteomics added considerable value over and above the information generated by whole genome sequencing, allowing for gene expression, not just gene presence to be considered. Specifically, in K. pneumoniae, we identified key differences between acrR and ramR regulatory mutations and compared the effects of OmpK36 Aspartate-Threonine or Glycine-Aspartate dipeptide porin insertions on susceptibility to cefepime and carbapenems. In P. aeruginosa, we identified differences in the gene expression effects of mexR versus nalC mutations and related these to differences in β-lactam MICs against isolates hyper-producing AmpC β-lactamase and or producing a metallo-β-lactamase.
Maturation of cortical endoplasmic reticulum clusters in the mouse oocyte: changes at fertilization
Authors: Wang Huizhen , Christenson Lane K. , Kinsey William H. 
Published: February 2022

AbstractOocytes from many invertebrate and vertebrate species exhibit unique endoplasmic reticulum specializations (cortical ER clusters) thought be essential for egg activation. In examination of cortical ER clusters, we observed they were tethered to previously unreported fenestrae within the cortical actin layer. Further, studies demonstrated sperm preferentially bind to plasma membrane overlying the fenestrae, establishing close proximity to underlying ER clusters. Moreover, following sperm-oocyte fusion, cortical ER clusters undergo a previously unrecognized global maturational change in volume, shape, and calreticulin content that persists through sperm incorporation, before dispersing at the pronuclear stage. These changes did not occur in oocytes from females mated with Izumo1 -/- males demonstrating that gamete fusion plays an important role in ER cluster maturation. In addition to these global changes seen at sites distant to the sperm, highly localized ER modifications were noted at the sperm binding site as cortical ER clusters surround the sperm head during incorporation, then form a diffuse cloud surrounding the decondensing sperm nucleus. This study provides the first evidence that cortical ER clusters interact with the fertilizing sperm, indirectly through a previous unknown lattice work of actin fenestrae, then directly during sperm incorporation. These observations raise the possibility that oocyte ER cluster-sperm interactions provide a competitive advantage to the oocyte, which may not occur during assisted reproductive technologies such as intracytoplasmic sperm injection.Summary StatementSperm-oocyte interactions stimulate global changes in cortical endoplasmic reticulum cluster structure as well as localized responses at the sperm binding site.
Crystal structure of an inorganic pyrophosphatase from Chlamydia trachomatis D/UW-3/Cx
Authors: Maddy Jasmine , Staker Bart L. , Subramanian Sandhya , Abendroth Jan , Edwards Thomas E. , Myler Peter J. , Hybiske Kevin , Asojo Oluwatoyin A. 
Published: February 2022

Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infections globally and is one of the most commonly reported infections in the United States. There is a need to develop new therapeutics due to drug resistance and the failure of current treatments to clear persistent infections. Structures of potential C. trachomatis rational drug-discovery targets, including C. trachomatis inorganic pyrophosphatase (CtPPase), have been determined by the Seattle Structural Genomics Center for Infectious Disease. Inorganic pyrophosphatase hydrolyzes inorganic pyrophosphate during metabolism. Furthermore, bacterial inorganic pyrophosphatases have shown promise for therapeutic discovery. Here, a 2.2 Å resolution X-ray structure of CtPPase is reported. The crystal structure of CtPPase reveals shared structural features that may facilitate the repurposing of inhibitors identified for bacterial inorganic pyrophosphatases as starting points for new therapeutics for C. trachomatis.
Motivating immersive BIM uptake through user attitude: analysis of initial solution using design science approach
Authors: Ramanayaka Chamil Dilhan Erik , Olatunji Oluwole Alfred , Weerasuriya Asiri U. 
Published: February 2022

PurposeBeyond a mandated use, rationales behind executive choice to accept or reject building information modelling (BIM) are ambiguous. BIM acceptance is complex for organisations, and existing decision-making models are impractical to make realistic choices. A practical solution must assist a holistic reflection of internal and external BIM success factors. Nevertheless, extant literature is largely focused on software use and awareness. Thus, this paper aims to suggest a novel framework for assessing firm readiness, aimed at facilitating BIM uptake.Design/methodology/approachExtant explanatory studies are inadequate in assessing the soft nature of BIM uptake. Thus, a design science research was employed as an alternative methodology. A means-end analysis was utilised for solution incubation, and cross-disciplinary reasoning, the strategy to establish valid solutions on firm readiness. Previous studies were reviewed on BIM, technology acceptance (generally) and complexity.Findings“Technology attitude” is presented as involving more holistic variables than a simplistic reliance on software use to mirror BIM acceptance. Technology acceptance model (TAM) is appropriate to explain BIM attitude attributes, but its current use is sub-optimal. Selective information processing and unconscious thought theory were integrated into TAM to explain attitude formation from multiple perspectives, resulting in a novel BIM attitude scale (BIMAS). Upon verification, the proposed framework will facilitate an objective authentication of biases that associate with BIM acceptance.Originality/valueWhilst BIM readiness is assessed largely with a primary focus on theory building, practical relevance must be at the forefront of BIM development. This study articulates that design science research can enhance the practical relevance of BIM adoption models. BIM acceptance attitude must be assessed through a verified scale contrary to the assessment of self-biases of executives in literature. BIMAS suggests a testable solution for this. Theory building research must be the future focus to enhance the relevance of this initial solution.
A novel multi-material topology optimization method for permanent magnet assisted synchronous reluctance motors
Authors: Hidaka Yuki 
Published: February 2022

Purpose
The purpose of this paper is to develop a multi-material topology optimization method for permanent magnet-assisted synchronous reluctance motors.

Design/methodology/approach
In the proposed method, the optimization procedure consists of two steps. In the first step, the entire rotor area was selected for the design region and the distribution of the core and air materials was optimized. In the second step, the design region was limited to the air region of the former solution and the distribution of magnets and cores or magnets and air was optimized.

Findings
Because of the two-step process of the proposed method, the design parameters can be reduced compared to the conventional method. As a result, this study can prevent the solution space from becoming more complex and superior solutions can be founded effectively.

Research limitations/implications
Since limited case study is denoted in this paper, much more case studies, for example, three-dimensional optimization problems, are needed to be discussed.

Practical implications
The optimal solutions obtained by the proposed method have a smaller magnet volume and higher average torque than that of the conventional method.

Originality/value
In the proposed methods, optimization methodology, which consists of two-steps process, is differed from the conventional method.
“It’s hard for them to even understand what we are saying”(.) Language and power in the multinational workplace
Authors: Kim Kyoungmi , Angouri Jo 
Published: February 2022

Purpose
The purpose of this paper is to discuss the role of language ideologies in negotiating organisational relationships in a Korean multinational company (MNC). By adopting an interactional sociolinguistics (IS) approach, this paper illustrates how language becomes part of a mechanism of negotiating group membership and of perpetuating or challenging power asymmetries through social and ideological processes.

Design/methodology/approach
This paper draws on interview data from an ethnographic case study of a Korean MNC to understand language ideologies in one working team. The interview data are analysed through an IS framework to connect the situated interaction to the broader social context.

Findings
This paper shows that participants’ discourse of linguistic differentiation becomes an interactional resource in challenging the organisational status quo. Linguistic superiority/inferiority is constructed through particular sequencing and the systematic production of a dichotomy between two groups – expatriate managers and local employees – at various levels of their company structure. Group membership is enacted temporarily in positioning the self and the others.

Originality/value
This paper offers a methodological contribution to international business language-sensitive research on language and power by conducting interactional analysis of interview talk. Through the lens of IS, it provides insights into how discourse becomes a primary site of negotiating power and status and a multi-level approach to the study of organisational power dynamics and the complex linguistic landscape of any workplace.
Quantifying the evolutionary mechanism of COVID-19 impact on international construction multi-projects: a risk driver perspective
Authors: Jin Feng , Xiang Wenwu , Ji Zheng , Zhang Bochen 
Published: February 2022

Purpose The purpose of this paper is to quantify the evolutionary mechanism of coronavirus disease 2019 (COVID-19) impact on international construction multi-projects.Design/methodology/approach From three concepts of complexity, stressor and detractor risks, a multi-project simulation model under COVID-19 outbreak is proposed to study the characteristics of contingency with three peaks of ordered and disordered states. Specially, COVID-19 brings forth mitigation fee, epidemic prevention fee, holdup fee and schedule delay fee of multi-projects. By integrating parametric model, Monte Carlo and chaos theory, a comparative analysis of its contingency with or without COVID-19 is conducted. Summarizing the simulated results, their total contingencies at certain risk tolerance are obtained at two status of static at one-time point and dynamic over time. Meanwhile, some major risks including detractors, complexities and stressors are screened out for mitigation, especially for epidemic prevention and control. Eventually, the real case is illustrated to demonstrate its validity.Findings It provides a quantitative analysis framework for the impact of epidemic, a once-in-a-century black swan event with a long tail, on construction multi-projects.Practical implications It conduct an effective model to quantify impacts of COVID-19 on international construction multi-projects for implementing effective counter-measures, which lay foundation for claims among different stakeholders.Originality/value The term of detractor risk is applied to describe COVID-19 and quantify its impact upon international construction multi-projects. Further, a hybrid model by integrating parametric model and Monte Carlo in type I/II model is proposed to simulate their contingencies at disordered states. Finally, the simulated outcomes of these models are used to guide effective risk control to meet the requirements by the client.
Returning to historic neighborhoods: exploring the role of architectural identity elements on the formation of physical identity
Authors: Farhad Sharareh , Maghsoodi Tilaki Mohammad Javad , Hedayati Marzbali Massoomeh 
Published: February 2022

Purpose
The study aims to evaluate the relationship among architectural identity, physical identity and neighborhood attachment in a residential neighborhood in the core of Sanandaj, Iran.

Design/methodology/approach
This quantitative study conducted a survey of 208 residents in a historic neighborhood. The study hypothesized the mediating role of physical identity on the relationship between architectural identity and neighborhood attachment. Structural equation modeling using analysis of a moment structures (AMOS) software was used to test the research hypotheses.

Findings
Research findings show the reliability and validity of the proposed model. The results indicate a significant relationship between architectural and physical identity and a significant direct relationship between physical identity and neighborhood attachment. However, no significant direct relationship is found between architectural identity and neighborhood attachment, and only a significant indirect effect is found through physical identity. Therefore, architectural identity elements contribute to increasing physical identity and subsequently attach to the neighborhood.

Originality/value
Given the necessity for revitalizing the neighborhoods in the historical core of the cities to prevent the migration from old neighborhoods and the destruction of the valuable fabrics, establishing emotional relationships between residents and the place and improving interactions among residents is required. The mental values of residents change over time. Although the identification and use of identity elements in residential buildings is a significant way to revitalize the historic residential fabrics, only a few studies have experimentally assessed the impact of architectural identity elements in the creation of physical identity on the residents’ attachment to the historic neighborhood.
Developing secondary schools as learning organisations: a systemic contribution
Authors: Barnard Peter Alexander 
Published: February 2022

PurposeThe purpose of this paper is threefold: first, to explain the link between traditional same-age school structure and the impact this has on a school’s capacity for individual and organisational learning; second, to explain why attempts to develop schools as learning organisations (LOs) invariably reify existing structures and practice, and finally, to provide an example of how and why schools that have adopted a multi-age form of organisation, a vertical tutoring (VT) system, have stumbled upon an embryonic form of LO.Design/methodology/approachThis conceptual paper draws on a critical review of the LO literature and its defining characteristics. The paper adopts a multi-disciplinary approach combining autopoiesis and complexity science to explore differences in learning capacity between traditional same-age schools (year or grade-based structure) and schools that have transitioned to multi-age organisation (vertical tutoring system).FindingsThe traditional form of same-age organisational “grammar” used in secondary schools is highly resistant to change, and any attempts at reform that fail to focus on organisation only reify existing systemic behaviour. VT schools change their form of organisation enabling them to create the capacity needed to absorb the unheard voices of participant actors (staff, students, and parents) and promote individual and organisational learning (constituent features of the LO).Originality/valueThis conceptual paper argues that for secondary schools to develop any semblance of an LO, they must abandon the restrictions on learning caused by their same-age form of organisation. The VT system provides the kind of organisational template needed.
The relationship between socioeconomic development, renewable energies and the innovative process
Authors: Sousa Saymon Ricardo de Oliveira , da Silva Wesley Vieira , Kaczam Fabíola , Cruz Nicholas Joseph Tavares da , Veiga Claudimar Pereira da , Zanini Roselaine Ruviaro 
Published: February 2022

Purpose
This paper aims to examine the relationships between socioeconomic development, renewable energy and the innovative process by providing: a descriptive analysis; a co-occurrence analysis of terms, thematic mapping and conceptual structure; and the typology of the textual corpus.

Design/methodology/approach
To analyze the relationship between “renewable energies, socioeconomic development and the innovative process,” it is necessary to build a theoretical foundation that contains the relevant scientific studies and reflects the current state of the art on the subject. For this, this study developed a systematic literature review (SLR) using the preferred reporting items for systematic reviews and meta-analyses research protocol to answer the relationship on the theme.

Findings
Research shows a global understanding of the need to invest in developing studies to reduce carbon dioxide emissions and improve economic growth. The main contributions lie in providing a typology of the state of the art, identifying the joint relationships between themes, insights into the key themes and indicating themes that must be developed. This study may also support future empirical studies as it provides a theoretical foundation for formulating hypotheses, which can be tested through qualitative and quantitative approaches.

Originality/value
The innovative character consists of addressing a shortage of SLRs on this theme. Thus, this paper fills this gap by providing a theoretical foundation for future scientific and academic knowledge generation. Furthermore, regarding the interdisciplinary aspects of this research as contributions, this paper presented different approaches and theoretical perspectives.
Construing drivers of firm competitiveness: coalescing pursuit of excellence with entrepreneurial competencies
Authors: Biswas Abhijeet 
Published: February 2022

PurposeThe burgeoning population has an adverse impact on the employability prospects in the emerging economies and has triggered entrepreneurial activities to steer this predicament. The purpose of this study is to explicate the major entrepreneurial competencies that drive firm competitiveness.Design/methodology/approachThree industrial estates were selected from the two major industrial districts of eastern Uttar Pradesh, India. The study used a cross-sectional design and responses from micro, small and medium enterprises (MSME) entrepreneurs were collected by employing a structured questionnaire. Structural equation modeling was applied to empirically validate the proposed hypotheses in the study.FindingsThe study discerns the enablers of firm competitiveness and pursuit of excellence. The results reveal that the need for achievement and locus of control competencies strikingly augment firm competitiveness while risk-taking capability and innovativeness competencies aggrandize pursuit of excellence competency. The study further adduces that pursuit of excellence partially mediates between the liaison of risk-taking capability and innovativeness on the one end and firm competitiveness on the other.Research limitations/implicationsThe study demonstrates the compelling determinants of firm competitiveness and pursuit of excellence that might embolden training institutes and banks to emphasize the identified competencies for enhancing firm competitiveness and advancing loans.Originality/valueThere is a paucity of literature on “pursuit of excellence” and “firm competitiveness” regarding the MSME sector in India. Therefore, the study contributes to the literature by synthesizing these constructs in an epigrammatic conceptual model. The study is distinctive because it is one of the rare studies to explore the mediating effects of the pursuit of excellence entrepreneurial competency.
From two-track to tessellation: a revised circulation of power model
Authors: Widdersheim Michael M. 
Published: February 2022

PurposeThe purpose of this project is to test and, if necessary, refine a model of the public sphere known as the circulation of power model. The model faces several criticisms and was applied in a case study only once. It has not yet been applied to an American context.Design/methodology/approachThis study uses the circulation of power model as a framework in a historical case study of a regional public library system in the United States. The temporal boundaries of the case are from 1924 to 2016.FindingsThis study resulted in a new and modified model called the tessellation model. New concepts in the tessellation model include circuits, tessellations, formal decisions and decision cycles. New distinctions in the model include narrowcast/broadcast and coalesced public/diffuse public.Research limitations/implicationsThe tessellation model and its associated concepts offer a new way to describe and analyze deliberative systems over time. The model requires further testing in other contexts.Originality/valueThe tessellation model is a new and validated way to describe the public sphere in an American political context.