bluuebunny/crossref_metadata_2025_split huggingface dataset:
The CDK7 inhibitor CT7001 (Samuraciclib) targets proliferation pathways to inhibit advanced prostate cancer
Authors: Constantin Theodora A. , Varela-Carver Anabel , Greenland Kyle K. , de Almeida Gilberto Serrano , Penfold Lucy , Ang Simon , Ormrod Alice , Ainscow Edward K. , Bahl Ash K. , Carling David , Fuchter Matthew J. , Ali Simak , Bevan Charlotte L. 
Published: June 2022

ABSTRACTBackgroundCurrent strategies to inhibit the androgen receptor (AR) are circumvented in castration-resistant prostate cancer (CRPC). Cyclin-dependent kinase 7 (CDK7) promotes AR signalling, in addition to established roles in cell cycle and global transcription regulation, together, providing a rationale for its therapeutic targeting in CRPC.MethodsThe antitumour activity of CT7001, an orally bioavailable CDK7 inhibitor, was investigated across CRPC models in vitro and in xenograft models in vivo. Cell-based assays and transcriptomic analyses of treated xenografts were employed to investigate the mechanism driving activity of CT7001, alone and in combination with the antiandrogen enzalutamide.ResultsCT7001 selectively engages with CDK7 in prostate cancer cells, causing inhibition of proliferation and cell cycle arrest. Activation of p53, induction of apoptosis, and suppression of transcription mediated by full-length and constitutively active AR splice variants contribute to antitumour efficacy in vitro. Oral administration of CT7001 represses growth of CRPC xenografts and significantly augments growth inhibition achieved by enzalutamide. Transcriptome analyses of treated xenografts indicate cell cycle and AR inhibition as the mode of action of CT7001 in vivo.ConclusionsThis study supports CDK7 inhibition as a strategy to target deregulated cell proliferation and demonstrates CT7001 is a promising CRPC therapeutic, alone or in combination with AR-targeting compounds.
Insights into the microevolution of SARS-ACE2 Interactions: In-silico analysis of glycosylation and SNP pattern
Authors: Madasu Pavan K , Maity Arpita , Ghosh Surya K. , Chandran Thyageshwar 
Published: June 2022

AbstractThe prefatory protein-glycan interaction and stabilizing protein-protein interaction of severe acute respiratory syndrome viruses with angiotensin-converting enzyme 2 play a significant role in complex formation thereby promoting endocytosis. The microevolution of SARS-CoV-2 over a period of time has a significant role in increasing the affinity of receptor-binding domain against angiotensin converting-enzyme 2. In the current study, we have corroborated the vitality of acquired SNPs over a period of time with increased affinity by using docking studies. The results indicate that the virus modulates the undesirable glycosylation sites by a series of substitution and deletion mutations. It uses bulky residues such as Tyr/Phe for dynamic arrest for quick stabilization of the complex, and Lys residues for stabilizing via hydrogen bond formation besides increasing the binding affinity to ease the cell entry.
Identifying and quantifying isoforms from accurate full-length transcriptome sequencing reads with Mandalorion
Authors: Volden Roger , Schimke Kayla , Byrne Ashley , Dubocanin Danilo , Adams Matthew , Vollmers Christopher 
Published: June 2022

AbstractThe Mandalorion tool, which we have continuously developed over the last 5 years, identifies and quantifies high-confidence isoforms from accurate full-length transcriptome sequencing reads produced by methods like PacBio Iso-Seq and ONT-based R2C2. In this manuscript, we introduce and benchmark Mandalorion v4 which further improves upon the already strong performance of Mandalorion v3.6 used in the LRGASP consortium challenge. By processing real and simulated accurate full-length transcriptome sequencing data sets, we show three main features of Mandalorion: First, Mandalorion-based isoform identification has very high Precision and maintains high Recall even when used in the absence of any genome annotation. Second, isoform read counts as quantified by Mandalorion show high correlation with simulated read counts. Third, isoforms identified by Mandalorion closely reflect the full-length transcriptome sequencing data sets they are based on.
Chloroplast activity provides in vitro regeneration capability in contrasting cultivars
Authors: Sirohi Parul , Chaudhary Chanderkant , Baliyan Suchi , Vishnoi Ritika , Mishra Sumit Kumar , Chaudhary Reeku , Waghmode Bhairavnath , Poonia Anuj Kumar , Germain Hugo , Sircar Debabrata , Chauhan Harsh 
Published: June 2022

AbstractExistence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants. We know little about why only some cultivars in crop plants are tissue culture friendly. In this study, tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency. Multiomics studies involving transcriptomics, proteomics, metabolomics, and biochemical analysis were performed using GP and D91 callus to unravel the regulatory mechanisms. Transcriptomics analysis revealed 1487 differentially expressed genes (DEGs), in which 795 DEGs were upregulated and 692 DEGs were downregulated in the GP-D91 transcriptome. Genes encoding proteins localized in chloroplast and involved in ROS generation were upregulated in the embryogenic calli of GP. Moreover, proteome analysis by LC-MSMS revealed 3062 protein groups and 16989 peptide groups, out of these 1586 protein groups were differentially expressed proteins (DEPs). Eventually, GC-MS based metabolomics analysis also revealed the higher activity of plastids and alterations in key metabolic processes such as sugar metabolism, fatty acid biosynthesis, and secondary metabolism. Higher accumulation of sugars, amino acids and metabolites corresponding to lignin biosynthesis were observed in GP as compared to D91.Highlights:Multi omics analysis revealed chloroplast play crucial role in providing in vitro regeneration capability in contrasting genotypes
Exploring key factors sustaining micro and small food, wine and hospitality firms through the COVID-19 crisis
Authors: Bressan Alessandro , Duarte Alonso Abel , Vu Oanh Thi Kim , O'Brien Seamus 
Published: June 2022

PurposeThe purpose of this study is to contribute to the understanding of how micro and small firm owners/managers cope with an extreme event, as this has implications on how firms make decisions. The study considers self-efficacy and stakeholder theory as tools to gain more in-depth knowledge.Design/methodology/approachThe perspectives of owners/managers of 308 micro and small firms operating in the food, wine and hospitality industries in Italy, one of the most affected nations, were drawn through an online questionnaire.FindingsThe importance of determination, passion, family support and a sense of responsibility towards internal and external stakeholders emerged as fundamental factors helping firms confront the crisis. Five theoretical dimensions that help explain how firm owners/managers make decisions to safeguard their firms during the COVID-19 crisis are identified. Three of these, “motivational”, “stepping up” and “firm-based”, are directly associated with tenets of self-efficacy theory, and two, “human-moral” and “entity-based”, with stakeholder theory. Further complementing this second contribution, a theoretical framework underlining conceptual and practical implications is proposed.Originality/valueThe study delves into the challenges and survival of a key group of firms facing an extreme crisis. The identified dimensions provide useful conceptual depth and practical insights that, together, form part of a proposed framework. For instance, the “human-moral” dimension reflects upon aspects that have wider implications, notably, for firms' employees and the wider society.
How do foodservice companies organize for inbound open innovation? Empirical evidence from a Dutch organization
Authors: Annosi Maria Carmela , Casprini Elena , Parra Hector 
Published: June 2022

PurposeThe aim of the paper is to analyze how actors in foodservice companies organize for inbound open innovation (OI).Design/methodology/approachThis paper conducted a case analysis of a large and successful foodservice company operating in the Dutch market. Furthermore, drawing on 18 interviews and archive data, we identified the main organizational practices involved in the implementation of inbound innovation activities and the ways they are embraced are defined.FindingsThe results provide a holistic view of the main organizational practices a foodservice company implemented at different organizational levels, to exploit external knowledge coming from third parties and to promote the sharing and recombination of knowledge resources within the organization. The identified organizational practices reveal the main interaction patterns between relevant internal actors and other external parties in the company network, as well as between actors on different hierarchical organizational levels which allows processing relevant innovation information and make relevant decisions about it.Research limitations/implicationsImplications are provided in terms of both theory and practice. This paper helps foodservice companies to create an internal organizational environment that supports the exploitation of customer knowledge.Originality/valueThere are few studies on how companies organize themselves for OI in general, and especially in the foodservice sector.
How A/E/C professionals accept BIM technologies in China: a technology acceptance model perspective
Authors: Zhao Yiwei , Sun Yindong , Zhou Qianqian , Cui Caiyun , Liu Yong 
Published: June 2022

PurposeThe aim of this paper is to research the acceptance mechanism of building information modeling (BIM) technology and to explore the differences among Architecture/Engineering/Construction (A/E/C) professionals with different individual characteristics. The proposed acceptance mechanism of BIM technology is intended to be used by industry stakeholders to propose decisions and measures, and improve the degree of BIM adoption.Design/methodology/approachTraditional hypothesis testing is adopted by the current study to empirically research the specific mechanism of A/E/C professionals accepting BIM technologies. In the one phase, a conceptual model based on technology acceptance model (TAM) and technology organization environment (TOE) theory was established and a large-scale questionnaire survey was conducted. In the other phase, structural equation modeling (SEM) was used to analyze acquired sample data, so as to empirically test the validity of the proposed linkage.FindingsThe results show first that perceived ease of use has no significant influence on perceived usefulness, and perceived usefulness has no significant effect on behavior intention as well. Second, BIM technical features and government BIM policies have positive effects on perceived usefulness, BIM technical features and organization supports have positive effects on perceived ease of use. Third, the BIM acceptance mechanism of A/E/C professionals is diverse from respondents with different characteristics, e.g. regions and working time.Originality/valueThe authors highlight the large sample size, as well as the nationwide context, of the questionnaire survey. Meanwhile, acceptance differences among A/E/C professionals with different demographic characteristics have been clarified using profound data and t-test. The findings of this study enrich the research on the acceptance mechanism of BIM technology, and contribute to relevant stakeholders taking targeted measures to promote the effective application of BIM technology nationwide.
Legal regulation of the state financial guarantees of medical services for the population: domestic and international experience
Authors: Lytvyn Nataliia A. , Berlach Anatolii I. , Kovalko Nataliia M. , Melnyk Alla A. , Berlach Hanna V. 
Published: June 2022

PurposeThe research topicality is stipulated by the need to study the legal regulation problems of the state financial guarantees of medical services for the population in Ukraine and to consider positive experience of the developed countries in this area. The aim of the article is to define the main trends in the legal regulation of healthcare in Ukraine; analyze the state financial guarantees of medical services for the population, and positive and negative aspects of the healthcare reform; to explore the international experience in medical services for its implementation to national law enforcement in the sphere of healthcare.Design/methodology/approachDuring the research, systematization and generalization of the positive international experience in the legal regulation of the state financial guarantees of medical services for the population were realized.FindingsThe analysis of the population's perception of healthcare innovations was carried out. The level of medical service was assessed. The results showed that in order to improve Ukrainian legislation on healthcare, it is necessary to remove absolute conflicts of normative legal acts; to establish public confidence in innovations and changes; to take into account international experience adapting it to specific features of Ukrainian legislation.Originality/valueThe defined theoretical and applied provisions constitute the practical significance for scholars and practicians, who deal with the legal regulation problems of the state financial guarantees of medical services for the population, university professors training highly qualified personnel, and all interested people concerned with healthcare.
Rebuilding disrupted supply chains: How can a self-organised social group facilitate supply chain resilience?
Authors: Lusiantoro Luluk , Pradiptyo Rimawan 
Published: June 2022

PurposeThis paper seeks to explore how a self-organised social group (SOSG) can facilitate supply chain resilience (SCRES) during an emergency condition.Design/methodology/approachA netnographic research was conducted on SONJO, an online SOSG emerging in response to problems in personal protective equipment (PPE) and food small businesses' supply chains (SCs) during the state of COVID-19 emergency in Yogyakarta, Indonesia. Qualitative data of 237,010 words were extracted from the group chats among 223 SONJO WhatsApp Group (WAG) members and were analysed using template analysis.FindingsThis paper reveals five communicative acts through which the SOSG facilitates SCRES, namely supply chain (SC) knowledge sharing, networking, bridging, mapping, and mindfulness. The enactment of these communicative acts could foster SC collaboration and help rebuild and sustain the SC operations during the critical period of the pandemic. The SOSG also facilitates the SC actors to be heedful of their responsive actions and risky operations.Practical implicationsThis paper emphasises the need for organisations to build and maintain relationships with social communities and to extend their social capital beyond their existing SC linkages as an alternative way to survive unexpected disruptions.Originality/valueThis paper offers a novel perspective to understand SCRES from an external force. It proposes that, in the face of a devastating disruption, SCRES is not a self-induced process and that the SOSG could play a pivotal role in rebuilding the disrupted SCs. It also shows how a humanitarian effort could help rebuild commercial SCs.
Is supplier involvement always beneficial for financial performance? The roles of ambidextrous innovations and product smartness
Authors: Wang Yu , Jia Tao , Chen Jinliang 
Published: June 2022

PurposeThe purpose of this paper is to investigate the performance impact of supplier involvement, based on the knowing processes and contingencies of knowledge-based view. Ambidextrous innovations (i.e. exploitative innovation and exploratory innovation) are taken as intermediary processes. Furthermore, product smartness is considered to clarify boundary conditions.Design/methodology/approachThe ordinary least squares regression was conducted, based on the two-source data collected from 125 high-tech firms in China.FindingsAmbidextrous innovations positively mediate the relationship between supplier involvement and financial performance. Product smartness weakens the indirect impact via exploratory innovation but not exploitative innovation.Originality/valueThis study reveals the knowledge application and recombination mechanisms of ambidextrous innovations to mediate between supplier involvement and financial performance. It also highlights digital encapsulation function of product smartness as a contingent factor.
Community structure determines the predictability of population collapse
Authors: Baruah Gaurav , Ozgul Arpat , Clements Christopher F. 
Published: June 2022

Abstract

Early warning signals (EWS) are phenomenological tools that have been proposed as predictors of the collapse of biological systems. Although a growing body of work has shown the utility of EWS based on either statistics derived from abundance data or shifts in phenotypic traits such as body size, so far this work has largely focused on single species populations.

However, to predict reliably the future state of ecological systems, which inherently could consist of multiple species, understanding how reliable such signals are in a community context is critical.

Here, reconciling quantitative trait evolution and Lotka–Volterra equations, which allow us to track both abundance and mean traits, we simulate the collapse of populations embedded in mutualistic and multi‐trophic predator–prey communities. Using these simulations and warning signals derived from both population‐ and community‐level data, we showed the utility of abundance‐based EWS, as well as metrics derived from stability‐landscape theory (e.g. width and depth of the basin of attraction), were fundamentally linked. Thus, the depth and width of such stability‐landscape curves could be used to identify which species should exhibit the strongest EWS of collapse.

The probability a species displays both trait and abundance‐based EWS was dependent on its position in a community, with some species able to act as indicator species. In addition, our results also demonstrated that in general trait‐based EWS were less reliable in comparison with abundance‐based EWS in forecasting species collapses in our simulated communities. Furthermore, community‐level abundance‐based EWS were fairly reliable in comparison with their species‐level counterparts in forecasting species‐level collapses.

Our study suggests a holistic framework that combines abundance‐based EWS and metrics derived from stability‐landscape theory that may help in forecasting species loss in a community context.
Assessment of screen‐recalled abnormalities for digital breast tomosynthesis versus digital mammography screening in the BreastScreen Maroondah trial
Authors: Li Tong , Lockie Darren , Clemson Michelle , Houssami Nehmat 
Published: June 2022

AbstractIntroductionAustralia's first population‐based pilot trial comparing digital breast tomosynthesis (DBT) and digital mammography (DM) screening reported detection measures in 2019. This study describes the trial's secondary outcomes pertaining to the assessment process in women screened with DBT or DM, including the type of recalled abnormalities and the procedures performed.MethodsWomen with suspected abnormalities at screening were recalled for further investigation. Outcome measures were number of lesions assessed, types of imaging findings recalled to assessment, and data on testing and assessment outcomes; these were reported using descriptive analyses of lesion‐specific data.ResultsA total of 274 lesions and 203 lesions were reported in the DBT‐screened and DM‐screened groups, respectively. There were a higher proportion of lesions depicted as calcifications (32.4% vs 21.3%), and a lower proportion of lesions depicted as asymmetrical densities (3.2% vs 15.7%) for DBT recalls than DM recalls. A lower proportion of DBT‐recalled lesions was assessed with additional mammography than DM‐recalled lesions (49.3% vs 93.1%). Higher proportions of DBT‐recalled lesions than DM‐recalled lesions were investigated with clinical breast examination (50.4% vs 39.9%), core needle biopsy (45.6% vs 28.6%) and open biopsy (4.0% vs 1.0%). Similar proportions of DBT‐ and DM‐recalled lesions were assessed using ultrasound (76.3% vs 71.4%).ConclusionAssessment of screen‐recalled lesions showed that, compared with DM, DBT found more benign and more malignant lesions, and generally required more procedures except for less additional mammography workup. These findings show that a transition to DBT screening changes the assessment workload.
Gamma knife radiosurgery for patients with brain metastases from non–small cell lung cancer: Comparison of survival between <5 and ≥5 metastases
Authors: Zhao Xu , Ding Shouluan , Zhang Ming , Wang Chengwei 
Published: June 2022

AbstractBackgroundCurrent evidence‐based guidelines support stereotactic radiosurgery (SRS) for patients with up to four brain metastases (BMs). However, debate continues about how many tumors may be treated by SRS alone.MethodsThis retrospective study included non–small cell lung cancer (NSCLC) patients with BMs treated with gamma knife as the initial treatment for cerebral lesions. The patients were followed up to obtain their survival information. The outcomes were statistically analyzed to compare the differences in survival between the <5 BMs and ≥5 BMs groups and to identify prognostic factors.ResultsA total of 77 patients were divided into two groups (54 patients with <5 BMs and 23 patients with ≥5 BMs). The median overall survival (OS) was 18.3 months in the <5 BMs group and 17.7 months in the ≥5 BMs group. The median intracranial progression‐free survival (IPFS) was 9.0 months in the <5 BMs group and 9.9 months in the ≥5 BMs group. There was no significant difference in OS and IPFS between the two groups. The multivariate analysis demonstrated that adenocarcinoma, controlled primary cancer, higher Karnofsky Performance Scale (KPS), and salvage treatment were independent prognostic factors favoring longer OS.ConclusionSRS alone as the initial treatment for NSCLC patients with more than four BMs was non‐inferior to SRS for those with one to four BMs in terms of OS and IPFS.