bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of 2 Patient-Reported Outcome Measures in Brachial Plexus Birth Injury
Authors: Vuillermin Carley , Eisenberg Katherine , Williams David , Tartarilla Ashley B. , Bauer Andrea , Waters Peter M. 
Published: February 2022

Background:
Patient-reported outcome (PRO) measures are integral to clinical practice. While new instruments bring updated methodology and delivery methods, they must be compared with existing measures to ensure that they are comparable with currently used instruments and allow ongoing comparison. In this study, we evaluated 2 PRO measures for patients with brachial plexus birth injury (BPBI): the legacy Pediatric Outcomes Data Collection Instrument (PODCI) and the newer Patient-Reported Outcomes Measurement Information System (PROMIS). We hypothesized that the PROMIS and PODCI would be correlated, with high convergent validity, and that PROMIS assessment would have higher discriminant validity and take less time to complete than the PODCI.

Methods:
A cross-sectional prospective study was undertaken at a tertiary referral center. Subjects (n = 50) completed the PODCI and PROMIS, both as short form (SF) and computer-adaptive testing (CAT) measures, in randomized order. Patient and parent questionnaires were completed according to subject age. Physicians completed upper-extremity (UE) physical function tests. Correlations between PODCI and PROMIS scores were analyzed to determine convergent validity, UE subscores were analyzed relative to physical function scores to assess discriminant validity, and completion times were calculated to determine survey time to completion.

Results:
High convergent validity was found between the parent-reported PODCI, PROMIS SF, and PROMIS CAT measures. A ceiling effect was found for the PODCI but not for the PROMIS. The PROMIS CAT had better discriminant validity than either the PROMIS SF or PODCI. Patient and parent outcome scores did not highly correlate. Survey time to completion varied by PRO measure and method of delivery.

Conclusions:
There was high convergent validity between the UE domains of the PROMIS and PODCI, with a lower time to completion for the PROMIS SF. Although results show that the PROMIS CAT may take longer to complete, there are notable benefits to its adoption, including high convergent validity with the PODCI and better discriminant validity than the PODCI and PROMIS SF measures.
Proliferative effect of aqueous extract of sea cucumber (Holothuria parva) body wall on human umbilical cord mesenchymal stromal/stem cells
Authors: Rasekh Poorya , Kameli Ali , Khoradmehr Arezoo , Baghban Neda , Mohebbi Gholamhossein , Barmak Alireza , Nabipour Iraj , Azari Hossein , Heidari Yaser , Daneshi Adel , Bargahi Afshar , Khodabandeh Zahra , Zare Shahrokh , Shirazi Reza , Almasi-turk Sahar , Tamadon Amin 
Published: February 2022

Abstract
Background: The sea cucumber potentials for stem cell proliferation induction and their mechanisms of bioactive compounds in its extract have been studied. Human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to aqueous extract of Holothuria parva body wall. Methods: Using GC-MS analysis on aqueous extract of H. parva, proliferative molecules were detected. The extract concentrations of 5, 10, 20, 40, and 80 µg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were used. MTT proliferation, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, effects of aqueous extract of H. parva and EGF on cell proliferation markers were detected. Computational modeling done to detect effective proliferative compounds in aqueous extract of H. parva. Results: MTT assay showed that the 10, 20, and 40 µg/mL aqueous extract of H. parva had proliferative effects on hUC-MSCs. Count of the cells treated with the 20 µg/mL of the extract was increased faster and higher than the control group (P<0.05). This concentration of extract did not have significant effects on hUC-MSCs viability. The cell cycle assay of hUC-MSCs showed that percent of cells in the G2 stage of the extract was biologically higher than the control group (P>0.05). Expression of the cyclin D1, cyclin D3, cyclin E, HIF-1α, and TERT were increased comparing with the control group. Moreover, expression of the p21 and PCNA decreased after treating hUC-MSCs with the extract. However, the CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of the cdk-4 and cdk-6 was decreased after treatment with the extract. Between the detected compounds, 1-methyl-4-(1-methylethenyl)-benzene showed better affinity to cdk-4 and p21 than tetradecanoic acid. Conclusions: The H. parva aqueous extract showed proliferative potential on the hUC-MSCs.
Upregulation of CCT3 Predicts Poor Prognosis and Promotes Cell Proliferation via Inhibition of Ferroptosis and Activation of EGFR Signaling in Lung Cancer
Authors: Wang Kun , He Jian , Tu Changling , Xu Hui , Zhang Xugang , Lv Yongchang , Song Chao 
Published: February 2022

Abstract
Background
Chaperonin containing TCP1 subunit 3 (CCT3) acts as an oncogene in cancers, whereas its role in lung cancer is poorly understood. This study investigated the clinical relevance and function of CCT3 in lung cancer.
Methods
Clinical relevance of CCT3 in lung cancer was analyzed based on TCGA database. qRT-PCR and Western blot assay were used to detect mRNA and protein expression, respectively. CCK8 and colony formation were performed to measure cell viability. Migration was detected by transwell. PI and PI/Annexin V-FITC assay kit were used to determine cell cycle and apoptosis, respectively. Luciferase activity was performed to check the whether CCT3 regulated slc7a11’s transcription activity. Ferroptosis was determined by incubating the cells with ferroptosis inducer, inhibitor and apoptosis inhibitor, followed by cell viability examination.
Results
We found that CCT3 was overexpressed in lung cancer tissues. Overexpression of CCT3 predicted poor prognosis in the patients. Loss-of-function and gain-of-function experiments demonstrated that CCT3 promoted the proliferation and colony formation of lung cancer cells. In addition, CCT3 knockdown suppressed cell cycle progression, cell migration and induced slc7a11-mediated cell ferroptosis, but not apoptosis. We also found that CCT3 activated EGFR and AKT. Gefitinib significantly reduced the viability of CCT3 overexpressed lung cancer cells, while had minimal effect on the proliferation of control cells, suggesting that CCT3 dictates the sensitivity of lung cancer cells to EGFR inhibition.
Conclusion
Our study demonstrates that CCT3 contributes to the proliferation and growth of lung cancer cells through inhibition of ferroptosis and activation of EGFR and AKT.
Gene Expression and DNA Methylation in Human Papillomavirus Positive and Negative Head and Neck Squamous Cell Carcinomas.
Authors: Stanković Snežana , Rich April , Anayannis Nicole , Cabarcas-Petroski Stephanie , Schramm Laura , Meneses Patricio I. 
Published: February 2022

Abstract
High-risk human papillomaviruses (HPV) are an important agent that cause head and neck squamous cell carcinomas (HNSCC). Besides viral induced cancers, tobacco use and heavy alcohol consumption are believed to cause DNA damage, promoting tumorigenesis. How gene expression and DNA methylation differ between HNSCC based on HPV status is yet unknown. We used publicly available gene expression and DNA methylation data from the Cancer Genome Atlas and compared HPV positive and HPV negative HNSCC. We used differential gene expression analysis, differential methylation analysis and a combination of these two analyses to identify differences. Differential expression analysis identified 1854 differentially expressed genes including PCNA, TNFRSF14, TRAF1, TRAF2, BCL2, and BIRC3. SYCP2 was identified as one of the top deregulated genes in the differential methylation analysis and in the combined differential expression and methylation analysis. Additionally, pathway and ontology analysis identified the extracellular matrix and receptor interaction pathway to be the most altered between HPV negative and HPV positive HNSCC groups. Combining gene expression and DNA methylation can help elucidate genes involved in HPV positive HNSCC tumorigenesis such as SYCP2 and TAF7L.
Observational study: Application of Therapy with Equine Serum for Patients Diagnosed with COVID-19 in Tucumán, Argentina
Authors: Chahla Rossana Elena , Ruiz Luis Medina , Contreras Miguel Enrique Ferré , Raya Mario , MsC Natalia Herrera , Ortega Eugenia Silvana , Goroso Daniel Gustavo , de Bruno María de los Ángeles Peral 
Published: February 2022

Abstract
Purpose: The objective of this work is to make an observational study of the usage in moderate or severe state COVID-19 patients of a new therapeutic commercial product obtained after immunization of horses: CoviFab® ELEA F(ab')2 fragmented equine immunoglobulins anti SARS-CoV2.Methods: Participant Centres depend on the Public Health System of Tucumán, Argentina were recruitment. Subjects were assigned to the Moderate Patient Group (MPG) and the Severe Patient Group (SPG), classified according to WHO criteria.In total, n = 84 were enrolled for this study. The subjects were divided into MPG and SPG. All participants were evaluated by physical examination and COVID-19 infection was diagnosed with positive RT-PCR. Each subject received two doses of 0.16 ml / kg, according to the subject's body weight. A generalized linear model with binomial distribution was adjusted for the number of symptoms. Data was analysed using proportion, bivariate and logistic regression. P-value was considered significant at the p< 0.05 threshold.Results: Both groups were similar in age, sex, and comorbidities. A higher proportion of patient with medical discharge was observed in MPG (91.4%) vs. SPG (55.3%) (p= 0.004). MPG showed 9 times more chance of receiving medical discharge than SPG (9.33 CI= [1.65, 52.81]; p= 0.012). Then, the chance to get medical discharge was independent of variables sex, age, and comorbidities. Conclusions: Treatment with Equine Serum in patients with moderate and severe disease of COVID-19 managed to slightly reduce hospitalization time. This treatment improved the clinical state to obtain medical discharge. The bivariate analysis showed 8 times more chance in MGP versus SGP to receive of medical discharge and this chance was independent of the pre-existent comorbidities.
A visual knowledge map analysis of mine fire research based on Citespace
Authors: Wang Feiran , Tan Bo , Chen Yue , Fang Xiyang , Jia Guowei , Wang Haiyan , Cheng Gang , Shao Zhuangzhuang 
Published: February 2022

Abstract
Mine fire has always been a serious disaster in coal industry, many academic achievements have poured out in the past two decades for solving this problem. In this study, visual analysis was conducted to grasp the hotspots and development trend of mine fire research. Papers that published in 1999-2020 were retrieved as the data basis from Web of Science, and Citespace was used to carry out knowledge map analysis. The results shown that number of papers has increased steadily since 2005 and achieved explosive growth since 2014. DENG J is the first published author among many scholars. China, the US, Australia are active areas in mine fire research and China University of Mining and Technology ranks first in this field. The highest co-occurrence frequency key word is “spontaneous combustion”. "INTERNATIONAL JOURNAL OF COAL GEOLOGY" and "FUEL" provide guidance for mine fire research. Fire prevention technology, low carbon, ecology and sustainable development are the hot research in recent years.
Characterization Of The Complete Mitochondrial Genome Of Sea Duck Mergus Serrator And Comparison With Other Anseriformes Species
Authors: Peng CHEN , Jiaqi LI , LI Hongbo , LIU Wei , Jianliang ZHANG 
Published: February 2022

Abstract
Background: As a key group in the Anatidae, the Mergini tribe (sea ducks) is strongly structured, with clear genetic assignments and phylogenetic relationships. The tribes also differ in terms of breeding strategy, longevity, and bionomic strategy. The Mergini tribe as an excellent tribe to investigate molecular adaptions to elevate metabolic burden associated with other life-history characteristics. Mitochondrial genome (mitogenome) plays a critical role of molecular evolution and phylogenetic. Phylogenetic analyses can help investigate the relationships between mitochondrial DNA (mtDNA) molecular evolution and metabolic performance.Results: The mitogenome of Mergus serrator was 16,627 bp in length, and its structure was circular. Annotation of the mitogenome presented 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and 1 control region. To determine the intraspecific evolutionary relationships among Anseriformes, the mitogenomes of 53 other Anseriformes species were examined concurrently. Among Anseriformes species, the shortest mitogenome belongs to Oxyura jamaicensis (15,914 bp) and the longest is that of Anseranas semipalmata (16,868 bp). The mean base composition of the mitogenome of Anseriformes was T (22.31 ± 0.51%), C (32.63 ± 0.64%), A (29.36 ± 0.64%), and G (15.71 ± 0.52%), indicating a slight specific bias towards A and C. AT content ranged of the mitogenome was from 50.27% to 55.31%, with an average value of 51.67 ± 1.10%, higher than the GC content (Table 1) and similar to that of birds in general (50.5% to 57.7%).Conclusions: The structure and gene arrangement of the genome were basically the same as those of Anseriformes species. The analyses indicated that the Anseriformes include the families Anseranatidae, Anhimidae, and Anatidae. Furthermore, Anatinae is composed of Aythyini, Anatini, Somaterini, and Mergini. M serrator was sister to M. merganser and M. squamatus, and this group belongs to Mergini. The results of selection analysis demonstrated different evolutionary rates between Mergini tribes and other tribes, and most protein-coding genes evolved under purifying selection except for the ND6 gene.
Lens Iris Diaphragm Retropulsion Syndrome: incidence, risk factors and management. A prospective study.
Authors: Hallali Gabriel , AUBERT Tristan , Souied Eric , GLACET-BERNARD Agnès 
Published: February 2022

Abstract
Purpose: The lens-iris diaphragm retropulsion syndrome (LIDRS) corresponds to a brutal deepening of the anterior chamber during phacoemulsification. LIDRS is painful for the patient and sometimes causes intraoperative complications. This study was designed to assess the preoperative risk factors of LIDRS. Setting: Monocentric study in the university department of ophthalmology, Intercommunal Hospital Center, Creteil, France. Design: Prospective open-label observational study. Methods: Preoperative parameters of consecutive patients who underwent cataract surgery were recorded. LIDRS occurrence was evaluated during surgery and classified into 3 stages. Except for the first patient, a simple and rapid maneuver to separate the iris from the capsule (capsular touch) was performed in all patients with LIDRS. Results: LIDRS occurred in 101 out of 205 included eyes: mild in 73.2% (74/101) , marked in 16.8% (17/101) and severe in 9.9% (10/101). LIDRS eyes had a longer axial length (p<0.01) and a thinner lens than the control group (p<0.01). Univariate logistic regression showed that the main predictive factors of LIDRS were long axial length, decrease lens thickness. LIDRS occurred in 83% (5/6) of eyes with prior vitrectomy. Complications occurred (zonular dehiscence), especially in vitrectomized eyes. The capsular touch allowed in almost all eyes to restore normal chamber depth and to prevent complications. Conclusions: Myopia and prior vitrectomy were the main risk factors of LIDRS. The capsular touch allowed to resolve LIDRS in almost all eyes. Analysis of preoperative factors can help the surgeon to identify patients at risk and to prevent patient pain and intraoperative complications associated with LIDRS.
What Factors Contribute to Moral Problems in Patient Care? A Scoping Review and Case Series of Ethics Consultations at a Somatic and a Psychiatric University Hospital in Switzerland
Authors: Schürmann Jan , Reiter-Theil Stella 
Published: February 2022

Abstract
Background
Health care professionals (HCPs) are regularly exposed to moral problems arising in patient care that may compromise quality of care. An understanding of risk factors and early indicators may help practitioners to address these problems early on. This study aims to provide an overview of moral risk factors in patient care. Risk factors known from the literature and those that have led to moral problems discussed in clinical ethics consultations (CECs) are examined. Characteristics, ethical issues and outcomes of CEC are also presented.
Methods
Scoping review on moral risk factors in patient care; CEC case series with semi-structured analysis of documentation (consultation reports, feedback forms, electronic patient records) from 204 CECs held between 2012 and 2020 at a somatic (USB) and a psychiatric (UPK) university hospital in Basel, Switzerland.
Results
Ninety-nine moral risk factors were identified from the literature referring to four risk areas: patient (41), family (12), health care professional (29) and system (17). Eighty-seven of these were documented at least once in CEC. The most prevalent factors were patient vulnerability (100%), absent or unclear hospital policies (97.1%), shift work (83.3%), inadequate understanding between patient and HCPs (73.5%), inadequate communication (66.2%), disagreement between patient and HCPs about care (58.8%), and multiple care teams (53.4%). The prevalence differs significantly depending on the medical specialty. CECs are requested as often in psychiatry as in somatic medicine. In the USB, ethical questions about life-sustaining treatment (27.8%), maternal-foetal conflicts (18.8%) and assisted reproductive technology (10.5%) are the most prevalent; in the UPK, questions about coercive measures (41.8%), risk and opportunity assessment (14.9%) and child welfare endangerment (13.4%) predominate. Overall, a consensus was almost always reached among the participants (98.5%) and the results implemented (93.9%). CECs were rated as helpful (95.0%) or partly helpful (5.0%).
Conclusions
CEC is useful in both somatic medicine and psychiatry to find consensual and helpful solutions to moral conflicts. There are highly prevalent and potent moral risk factors in all dimensions relevant in clinical care that can be used to prevent moral problems. Further empirical research is needed to create different risk profiles specific to the medical specialities.
Observations of a magnetic dip in a quiescent prominence foot
Authors: Chen Huadong , Ma Suli , Su Yingna , Zhou Guiping , Priest Eric , Shen Yuandeng , Xia Chun , Tu Weining , Fletcher Lyndsay , Zhang Jun 
Published: February 2022

Abstract
Solar prominences (or filaments) are cooler and denser plasma suspended in the much hotter and rarefied solar corona. When viewed on the solar disc filament barbs or feet protrude laterally from filament spine. When viewed at solar limb, they extend down to the chromosphere. For a long time, the magnetic field orientation of barbs has remained a mystery due to the paradox that the barbs possess vertical fine structures and flows but are likely to be supported in a horizontal magnetic field. Here we present highly suggestive observations of a magnetic dip in a quiescent prominence foot with an upward-curved field. That is indicated by the horizontal bidirectional outflows probably produced by magnetic reconnection between the fields of a tiny erupting filament and those in a prominence foot. The observations shed light on the field structure of prominences which is crucial for the instability that accounts for the eruption of prominences and coronal mass ejections.
Artificial Intelligence Based Maintenance Framework for Industrial Machinery
Authors: Al-Fahad Imad Obaid , Hmood Haider S. 
Published: February 2022

Abstract
With the constant evolution of e-manufacturing technologies, there is a clear trend for e-maintenance that involves the integrating of ICT (Information and communication technologies) within the maintenance strategy. This leads to highly sophisticated and complex machinery, which increases the demand for expertise. Unfortunately, a company could always lose the expertise due to experts’ retirement, change of occupation or death. This motivates us in this work to develop an e-maintenance model that enables organizations to exploit expert’s knowledge in the process of machine fault diagnosis. This paper focuses on the building of a Knowledge-Based System (KBS) in order to capture the experts’ knowledge to be permanently kept and cannot be disparaged due to lack of practice. An optimal AI-based tool is proposed that aims at accurate values to retrieve information from KBS, which describes the alarms to diagnose the failure of the machine. An accurate analysis is carried out that yields insight into the impact of KBS on the ability of fault diagnosis. The results illustrate the high-performance of the proposed approach in handling the KBS's data associated.
Motivators of impulsivity to smoke waterpipe tobacco among Nigerian youths: the moderating role of social media normalisation of waterpipe tobacco
Authors: Adu Agatha Oluwafunmilayo , Ismail Nurzali , N Shuhaida Md 
Published: February 2022

Abstract
Background: Impulsivity is a formidable cause of waterpipe tobacco smoking among the youths, however, it is less well studied among African youths. Using PRIME behavioural theory, this study aimed to develop a model that examines the motivators of impulsivity to smoke waterpipe tobacco in linkage to the moderating role of social media normalisation of waterpipe tobacco among youths in Nigeria. Methods: Data were drawn from 695 youths who smoke waterpipe tobacco across six Nigerian universities in the south-west zone using the chain-referral sampling procedure. Descriptive analyses of the obtained data were carried out using the Statistical Package for Social Sciences (SPSS) version 25. The constructs in the developed model were validated through Partial Least Squares Structural Equation Modelling (PLS-SEM) in SmartPLS version 3.Results: Among the Nigerian youths who smoke waterpipe tobacco, intention (β=0.442, P<0.001) is the strongest motivator of impulsivity to smoke waterpipe tobacco as compared to positive evaluations (β=0.302, P<0.001). Additionally, social media normalisation of waterpipe tobacco acts as a moderator that strengthens the relationship between intention and impulsivity (β=0.287, P<0.01) as well as between positive evaluations and impulsivity (β=0.186, P< 0.01) among the youths.Conclusion: Willingness greatly instigates the Nigerian youths’ impulsivity to smoke waterpipe tobacco, and social media normalisation of waterpipe tobacco also considerably increases their impulsivity to smoke waterpipe tobacco. Youth-focused educative waterpipe tobacco cessation-oriented programmes that will utilise diverse constructive-based learning approaches like illustrative learning and counselling, are needed to enlighten and encourage the Nigerian youths on the importance of shunning the desirability to smoke waterpipe tobacco.