bluuebunny/crossref_metadata_2025_split huggingface dataset:
Balancing Act: Precision Medicine and National Security
Authors: DiEuliis Diane , Giordano James 
Published: December 2021

ABSTRACT
Developments in genetics, pharmacology, biomarker identification, imaging, and interventional biotechnology are enabling medicine to become increasingly more precise in “personalized” approaches to assessing and treating individual patients. Here we describe current scientific and technological developments in precision medicine and elucidate the dual-use risks of employing these tools and capabilities to exert disruptive influence upon human health, economics, social structure, military capabilities, and global dimensions of power. We advocate continued enterprise toward more completely addressing nuances in the ethical systems and approaches that can—and should—be implemented (and communicated) to more effectively inform policy to guide and govern the biosecurity and use of current and emerging bioscience and technology on the rapidly shifting global stage.
A Review of Genome-Based Precision Medicine Efforts Within the Department of Defense
Authors: Poon Lucas , Por Elaine D , Cho Hyun Joon , Oliver Thomas G 
Published: December 2021

ABSTRACT

Introduction
Providing patient-specific clinical care is an expanding focus for medical professionals and researchers, more commonly referred to as personalized or precision medicine. The goal of using a patient-centric approach is to provide safer care while also increasing the probability of therapeutic success through careful consideration of the influence of certain extrinsic and intrinsic human factors in developing the patient care plan. Of increasing influence on patient care is the phenotype and genotype information gathered from employing various next-generation sequencing methods. Guided by and partnered with our civilian colleagues, clinical components within the DoD are embracing and advancing genomic medicine in many facets—from the bench to the bedside—and in many therapeutic areas, from Psychiatry to Oncology. In this PubMed-based review, we describe published clinical research and interventions within the DoD using genome-informed data and emphasize precision medicine efforts in earlier stages of development with the potential to revolutionize the approach to therapeutics.

Materials and Methods
The new PubMed database was searched for articles published between 2015 and 2020 with the following key search terms: precision medicine, genomic, pharmacogenetic, pharmacogenomic, US military, and Department of Defense.

Results
Eighty-one articles were retrieved in our initial search. After screening the abstracts for studies that only involved direct testing of (or clinical interaction with) active duty, Reserve, National Guard, or civilian personnel working within the DoD and excluding any epidemiological or microbial isolation studies, seven were included in this review.

Conclusion
There are several programs and studies within the DoD, which investigate or use gene-based biomarkers or gene variants to deliver more precise clinical assessment and treatment. These genome-based precision medicine efforts aim to optimize the clinical care of DoD beneficiaries, particularly service members in the operational environment.
Operating Room Waste Generated Across Otolaryngology Cases
Authors: Ryan Mathew T , Malmrose Jonathan , Riley Charles A , Tolisano Anthony M 
Published: December 2021

ABSTRACT

Background
Hospital waste adds to the ecological footprint of the healthcare system, whereas inattention to recyclables may increase costs. The primary objective of this study was to assess the amount of recyclable and nonrecyclable intraoperative waste produced across representative otolaryngology surgical procedures.

Methods
Representative surgical cases across four otolaryngology subspecialties at a tertiary care military medical institution were prospectively identified. Waste was collected, divided, and weighed across two categories: recyclable and nonrecyclable. This study was performed in conjunction with a hospital-approved quality improvement project.

Results
The study included 22 otolaryngology surgeries performed across four otolaryngology subspecialties: facial plastics, pediatrics, otology, and head and neck oncology. Overall, 197.4 kg of waste was collected of which 40.2 kg (20%) was recyclable and 157.2 kg (80%) was nonrecyclable. An average of 1.8 kg of recyclable materials and 7.1 kg of nonrecyclable materials were collected per case.

Conclusion
This study supports that otolaryngology surgical procedures generate a significant amount of waste, a large component of which is recyclable. It highlights the need for proper disposal of this waste and the implementation of a recycling program at our institution with the potential for both ecologic and economic benefits.
Horizontally Acquired Cellulases Assist the Expansion of Dietary Range inPristionchusNematodes
Authors: Han Ziduan , Sieriebriennikov Bogdan , Susoy Vladislav , Lo Wen-Sui , Igreja Catia , Dong Chuanfu , Berasategui Aileen , Witte Hanh , Sommer Ralf J 
Published: December 2021

AbstractHorizontal gene transfer (HGT) enables the acquisition of novel traits via non-Mendelian inheritance of genetic material. HGT plays a prominent role in the evolution of prokaryotes, whereas in animals, HGT is rare and its functional significance is often uncertain. Here, we investigate horizontally acquired cellulase genes in the free-living nematode model organism Pristionchus pacificus. We show that these cellulase genes 1) are likely of eukaryotic origin, 2) are expressed, 3) have protein products that are secreted and functional, and 4) result in endo-cellulase activity. Using CRISPR/Cas9, we generated an octuple cellulase mutant, which lacks all eight cellulase genes and cellulase activity altogether. Nonetheless, this cellulase-null mutant is viable and therefore allows a detailed analysis of a gene family that was horizontally acquired. We show that the octuple cellulase mutant has associated fitness costs with reduced fecundity and slower developmental speed. Furthermore, by using various Escherichia coli K-12 strains as a model for cellulosic biofilms, we demonstrate that cellulases facilitate the procurement of nutrients from bacterial biofilms. Together, our analysis of cellulases in Pristionchus provides comprehensive evidence from biochemistry, genetics, and phylogeny, which supports the integration of horizontally acquired genes into the complex life history strategy of this soil nematode.
SAPHO syndrome and pustulotic arthro-osteitis
Authors: Kishimoto Mitsumasa , Taniguchi Yoshinori , Tsuji Shigeyoshi , Ishihara Yoko , Deshpande Gautam A , Maeda Keizo , Okada Masato , Komagata Yoshinori , Kobayashi Satomi , Okubo Yukari , Tomita Tetsuya , Kaname Shinya 
Published: December 2021

ABSTRACTSynovitis-Acne-Pustulosis-Hyperostosis-Osteitis (SAPHO) syndrome is a rare inflammatory osteoarticular disorder, which encompassed many diseases, including pustulotic arthro-osteitis (PAO). Musculoskeletal manifestations, including osteitis, synovitis, and hyperostosis, are the hallmarks of the SAPHO syndrome and affect a variety of regions of the body. Recent survey indicated that more than 80% of cases of SAPHO syndrome in Japan were PAO, originally proposed by Sonozaki et al. in 1981, whereas severe acne was the most commonly reported skin ailment amongst participants with SAPHO syndrome in Israel. Prevalence of SAPHO syndrome remains unavailable, whereas the prevalence of palmoplantar pustulosis (PPP) was reported to be 0.12% in Japan, and 10–30% of patients with PPP had PAO. SAPHO syndrome and PAO are predominantly found in patients in the third through fifth decades of life, and a female predominance is seen in both groups. The diagnosis is typically made by a rheumatologist or dermatologist. Identification of a variety of the clinical, radiological, and laboratory features outlined, as well as diagnostic criteria, are used to make the diagnosis. Goals of treatment seek to maximize health-related quality of life, preventing structural changes and destruction, and normalizing physical function and social participation. Finally, we review the non-pharmacological and pharmacological managements.
A Small but Significantly Greater Incidence of Inflammatory Heart Disease Identified After Vaccination for Severe Acute Respiratory Syndrome Coronavirus 2
Authors: Knowlton Kirk U , Knight Stacey , Muhlestein Joseph B , Le Viet T , Horne Benjamin D , May Heidi T , Stenehjem Edward A , Anderson Jeffrey L 
Published: December 2021

Abstract

Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are being administered on an unprecedented scale. Assessing the risks of side effects is needed to aid clinicians in early detection and treatment. This study examined the risk of inflammatory heart disease, including pericarditis and myocarditis, after SARS-CoV-2 vaccination.

Methods
Intermountain Healthcare patients with inflammatory heart disease from December 15, 2020 to June 15, 2021, and with or without preceding SARS-CoV-2 vaccinations, were studied. Relative rates of inflammatory heart disease were examined for vaccinated patients compared to unvaccinated patients.

Results
Of 67 patients identified with inflammatory heart disease, 21 (31.3%) had a SARS-Cov-2 vaccination within the previous 60 days. Overall, 914 611 Intermountain Healthcare patients received a SARS-CoV-2 vaccine, resulting in an inflammatory heart disease rate of 2.30 per 100 000 vaccinated patients. The relative risk of inflammatory heart disease for the vaccinated patients compared to the unvaccinated patients was 2.05 times higher rate within the 30-day window (P = .01) and had a trend toward increase in the 60-day window (relative rate = 1.63; P = .07). All vaccinated patients with inflammatory heart disease were treated successfully with 1 death related to a pre-existing condition.

Conclusions
Although rare, the rate of inflammatory heart disease was greater in a SARS-CoV-2-vaccinated population than the unvaccinated population. This risk is eclipsed by the risk of contracting coronavirus disease 2019 and its associated, commonly severe outcomes. Nevertheless, clinicians and patients should be informed of this risk to facilitate earlier recognition and treatment.
Postural Maintenance Is Associated With Walking Ability in People Receiving Acute Rehabilitation After a Stroke
Authors: Wang Ching-Yi , Chen Yueh-Chi , Wang Chun-Hou 
Published: December 2021

Abstract

Objectives
The Postural Assessment Scale for Stroke Patients (PASS) assesses the ability of people post stroke to maintain or change a given posture from lying to standing, and the items on which people with different walking status perform differently may suggest potential interventions. The purpose of this study was to (1) examine the association of PASS scores at admission for acute rehabilitation with walking status at admission and 3 months post stroke (3 M), and (2) identify PASS items that discriminate walking status.

Methods
In this prospective observational study, 93 people post stroke were assessed with the PASS and a 2.44-m gait speed test at admission, with walking status assessed by telephone interview at 3 M. Those who could walk over a 2.44-m distance without the assistance of a walking aid or another person were considered to be independent in walking; others were considered to be dependent. Those who were dependent at admission were divided into the “regained independence” and “remained dependent” groups based on their status at 3 M. The association of the PASS at admission with 3 levels of walking status (independent at admission, regained independence, and remained dependent) was examined using the Kruskal-Wallis test. For those dependent at admission, the association of PASS score at admission with walking status at 3 M was examined using logistic regression and receiver operating curve analysis.

Results
PASS scores at admission differed significantly across the 3 walking status groups and were significantly associated with walking status at 3 M (odds ratio = 0.864; 95% CI = 0.798–0.935) over and above length of stay. People post stroke who were dependent at admission and had PASS scores ≥22 were more likely to regain independence at 3 M. Nine PASS items differed among the 3 groups.

Conclusions
PASS score is significantly associated with walking status at admission and at 3 M. The identified 9 items suggest possible interventions for acute rehabilitation.

Impact
This study identified 9 PASS items that could guide clinicians in selecting interventions for acute rehabilitation.
GAMMA SPECTROMETRY AND NEUTRON ACTIVATION ANALYSIS OF TOOT OILFIELD WELLS OF DATTA FORMATION, UPPER INDUS BASIN, DISTRICT ATTOCK, PUNJAB, PAKISTAN
Authors: Shah Zawwar Hussain , Siddique Naila , Wazir Zafar , Batool Nafeesa , Nusrat Muhammad , Somaily Hamoud H 
Published: December 2021

Abstract
Present study was aimed to investigate trace elemental composition and possible elemental correlation with depth of some Pakistani wells. Exploratory oil wells of Toot oilfield from Potwar region of upper Indus basin were selected for this purpose. Samples of well cuttings and soil sections were collected with the assistance of Oil and Gas Development Company Limited. Elemental analyses were performed using instrumental neutron activation analysis. This study is the first of its kind to report detailed elemental profile of Toot oilfield, Attock, Punjab, Pakistan, via instrumental neutron activation analysis (INAA). Source formation of Toot oilfield is characterized by Jurassic Datta formation. Around 19–26 elements were identified by INAA. Major, minor and trace elemental concentrations varied with depths along the sampling lines. Data analysis was performed by the application of principal component analysis (PCA). PCA was useful in differentiating between surface and depth samples. Elemental volumes of different wells were established through bi-plots. In extension, naturally occurring radioactive materials and technologically enhanced naturally occurring radioactive materials were also determined for the wells of Toot oilfield. Annual dose rates and activity levels were calculated in oil well formations. Measurements of annual dose rates fall within safe limits, indicating anodyne atmosphere. Contamination indices, such as enrichment factor with respect to Al, geo accumulation index (Igeo), pollution index and integrated pollution index, were also determined. Cr and Zn were found to cross the safe limits, which may be due to the local industrial and anthropogenic activities.
‘A Healthy Sex Life’: Love, Marriage and Sexual Knowledge in Franco’s Spain (1960–1975)
Authors: García-Fernández Mónica 
Published: December 2021

Summary
This article aims to explore changes in the marital sex advice that was available in Spain during the last decade of the Franco Regime (1939–75). It pays particular attention to the representation of female sexuality, which was shaped by fluctuating ideas on the relationship between sexual pleasure, love and contraception. The study looks at a variety of sources and ponders the relationships between different types of discourses: male- and female-defined; religious and medical; popular and intellectual; expert and lay. These include marriage manuals, intellectual essays and women’s magazines. These sources suggest that paying attention to the means by which sexual knowledge is conveyed, to the context in which it is disseminated, and to the subtle changes in the agents with the authority to communicate it gives us insight into how sexual knowledge is distributed, received and redefined.
Hypocretin in locus coeruleus and dorsal raphe nucleus mediates inescapable footshock stimulation (IFS)-induced REM sleep alteration
Authors: Lo Yun , Yi Pei-Lu , Hsiao Yi-Tse , Chang Fang-Chia 
Published: December 2021

Abstract
Hypocretin (hcrt) is a stress-reacting neuropeptide mediating arousal and energy homeostasis. An inescapable footshock stimulation (IFS) could initiate the hcrt release from the lateral hypothalamus (LHA) and suppresses rapid eye movement (REM) sleep in rodents. However, the effects of the IFS-induced hcrts on REM-off nuclei, the locus coeruleus (LC) and dorsal raphe nucleus (DRN), remained unclear. We hypothesized that the hcrt projections from the LHA to LC or DRN mediate IFS-induced sleep disruption. Our results demonstrated that the IFS increased hcrt expression and the neuronal activities in the LHA, hypothalamus, brainstem, thalamus, and amygdala. Suppressions of REM sleep and slow wave activity during non-REM (NREM) sleep caused by the high expression of hcrts were blocked when a nonspecific and dual hcrt receptor antagonist was administered into the LC or DRN. Furthermore, the IFS also caused an elevated innate anxiety, but was limitedly influenced by the hcrt antagonist. This result suggests that the increased hcrt concentrations in the LC and DRN mediate stress-induced sleep disruptions and might partially involve IFS-induced anxiety.
A National Survey of Collaboration Between Nurse Faculty Members and Staff Nurses in the Clinical Learning Environment
Authors: Hooven Katie 
Published: December 2021

Abstract

AIM
The purpose of this study was to measure collaboration in the clinical learning environment.

BACKGROUND
Clinical learning, which is essential for nursing education, allows students to integrate what they have learned in the classroom in a complex social context with an interactive network of forces. Stakeholders are nursing students, nursing faculty, and staff nurses who must collaborate in providing an effective learning environment for students.

METHOD
Quantitative data were collected via the Collaboration in the Clinical Learning Environment tool, an author-developed 24-item Likert scale designed to measure the amount of collaboration between staff nurses and nursing faculty members in the clinical learning environment.

RESULTS
Three groups (n = 882) were surveyed: staff nurses, nurse faculty, and nurses who worked concurrently in academia and practice.

CONCLUSION
This study adds to the literature on collaboration between nursing faculty members and staff nurses. It also highlights areas for improvement for both parties.
Bilinguals Show Proportionally Greater Benefit From Visual Speech Cues and Sentence Context in Their Second Compared to Their First Language
Authors: Chauvin Alexandre , Phillips Natalie A. 
Published: December 2021

Objectives:
Speech perception in noise is challenging, but evidence suggests that it may be facilitated by visual speech cues (e.g., lip movements) and supportive sentence context in native speakers. Comparatively few studies have investigated speech perception in noise in bilinguals, and little is known about the impact of visual speech cues and supportive sentence context in a first language compared to a second language within the same individual. The current study addresses this gap by directly investigating the extent to which bilinguals benefit from visual speech cues and supportive sentence context under similarly noisy conditions in their first and second language.

Design:
Thirty young adult English–French/French–English bilinguals were recruited from the undergraduate psychology program at Concordia University and from the Montreal community. They completed a speech perception in noise task during which they were presented with video-recorded sentences and instructed to repeat the last word of each sentence out loud. Sentences were presented in three different modalities: visual-only, auditory-only, and audiovisual. Additionally, sentences had one of two levels of context: moderate (e.g., “In the woods, the hiker saw a bear.”) and low (e.g., “I had not thought about that bear.”). Each participant completed this task in both their first and second language; crucially, the level of background noise was calibrated individually for each participant and was the same throughout the first language and second language (L2) portions of the experimental task.

Results:
Overall, speech perception in noise was more accurate in bilinguals’ first language compared to the second. However, participants benefited from visual speech cues and supportive sentence context to a proportionally greater extent in their second language compared to their first. At the individual level, performance during the speech perception in noise task was related to aspects of bilinguals’ experience in their second language (i.e., age of acquisition, relative balance between the first and the second language).

Conclusions:
Bilinguals benefit from visual speech cues and sentence context in their second language during speech in noise and do so to a greater extent than in their first language given the same level of background noise. Together, this indicates that L2 speech perception can be conceptualized within an inverse effectiveness hypothesis framework with a complex interplay of sensory factors (i.e., the quality of the auditory speech signal and visual speech cues) and linguistic factors (i.e., presence or absence of supportive context and L2 experience of the listener).
Outpatient Bryant’s Overhead Traction Does Not Affect the Rate of Open Reduction or Avascular Necrosis in Developmental Dislocation of the Hip
Authors: Elerson Emily E. , Martin Benjamin D. , Muchow Ryan D. , Pierce William A. , Jo Chan-Hee , Hinds Sean A. , Birch John G. 
Published: December 2021

Background:
The efficacy of preliminary traction to increase the likelihood of closed reduction and/or decrease the incidence of avascular necrosis in the management of developmental dysplasia of the hip (DDH) is controversial. We sought to document compliance with and effectiveness of Bryant’s outpatient traction in patients with idiopathic DDH.

Methods:
Patients presenting between 6 and 24 months of age with idiopathic irreducible DDH were prospectively enrolled in the study. Prereduction outpatient traction was prescribed at participating surgeons’ preference and parents’ expressed willingness to comply with a traction protocol of at least 14 hours/day for 4 weeks. Traction hours were documented using a validated monitor; parents also reported average daily usage. Rate of successful closed reduction and evidence of capital epiphyseal growth disturbance 1 year’ and 2 years’ postreduction were documented.

Results:
Ninety-six patients with 115 affected hips were enrolled. Reliable recorded traction hours were obtained in 31 patients with 36 affected hips. Defining compliance as 14 hours/day average use, 14 of 31 patients (45.2%) were compliant, 2 (6.5%) admitted noncompliance, while 15 (48.2%) claimed to be compliant, but were not. Overall, 68/115 hips (59.0%) were closed reduced. Age at treatment was the only demographic characteristic associated with an increased incidence of closed reduction (11.7 vs. 14.6 mo, P<0.01). Successful closed reduction was achieved in 10/16 hips (62.5%) of compliant patients, 12/20 (60.0%) of noncompliant patients, and 43/72 (59.7%) of no-traction patients. Irregular ossific nucleus development was noted 1-year postindex reduction in 5/16 (31.3%) of complaint-patient hips and 25/92 (27.2%) of noncomplaint and no-traction hips. Distorted proximal femoral epiphysis was noted at 2 years postreduction in 2/15 hips (13.3%) of compliant patients and 15/52 hips (28.8%) in noncompliant and no-traction patients. None of these differences was statistically significant.

Conclusions:
Parent-reported use of outpatient traction is unreliable. Four weeks of outpatient overhead Bryant’s traction did not affect the rate of closed reduction or avascular necrosis in late-presenting DDH in this cohort.

Level of Evidence:
Level II—prospective cohort.