bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of recurrence of HCC after TACE using enhanced CT heterogeneity assessment
Authors: Wang Ru , Kou Qiqi , Dou Lina 
Published: November 2022

Abstract
Purpose: To evaluate the value of enhanced computed tomography（CT）heterogeneity in predicting early recurrence of patients with hepatocellular carcinoma (HCC) after transarterial chemoembolization (TACE).
Methods: Forty-seven HCC patients (40M/7F) were included in the study. Tumor histogram and texture analysis were performed on contrast-enhanced CT imaging prior to TACE. The clinical diagnosis of HCC at recurrent stages was based on the criteria of the American Association for the Study of Liver Diseases (AASLD) or biopsy. Two parameters of HCC heterogeneity (histogram and texture) were compared in the Early Recurrence (ER) and the Non-Early Recurrence (Non-ER) groups. Analysis was applied to both single-slice ROI and whole-tumor volumetric VOI. Receiver operating characteristic (ROC）was calculated to determine the ability of the parameters to differentiate between ER and non-ER groups.
Results: 27 patients with ER and 20 patients with NER. The largest diameters of tumors in ER group were significantly larger than NER group( P＜0.001). Texture results for ROI and VOI analyses were similar. In the histogram analysis, the 50th percentile of pixel intensity predicted early recurrence with a sensitivity (Se) of 92.6%. In texture analysis, entropy, mean, and inhomogeneity were significantly associated with early recurrence( P＜0.05) but not skewness and kurtosis (P＞0.05). Inhomogeneity had the highest diagnostic specificity (95%).
Conclusions: This study shows that CT histogram and texture features are significantly different in ER group from Non-ER Group.
Molecular Finger Printing of Thirteen Pakistani Mid-season Mango (Mangifera Indica L.) Varieties by Using Ssr Markers
Authors: Rauf Sheeba , Sharif Hamza , Babar Muhammad , Gul Almass , Shaukat Faiza , Bukhari Birra , Mehvish Ambreen , Raza Samad , Kiran Sidra , Qureshi Muhammad Ahsan , Sharif Naseem , Bukhari Syed Inam Ullah Shah 
Published: November 2022

AbstractPresent study was performed to identify the genetic make-up of thirteen mid-season mango verities i.e.Zardalu, Banganpali, Taimuria, Saleh Bhai, Badia Muna Syed, Intikhab, Anmole, Bombay Alphanso, Ghulab Khas, Pan, Lahotia, Kachnaliwala, and Jo villagey by applying seventy SSR markers, data collected on genetic diversity estimation bifurcated the varieties into three clusters. cluster 1, enclosed three varieties namely Bombay Alphonso, Intikhab, and Anmole together in genetic resemblance. Similarly, the cluster 2 assembled seven varieties i.e. Jo Village, Taimuria, Zardalu, Bagan Palli, Saleh Bhai, Badia and Munna Syed, making it the larger cluster. Lastly, the genetic similarity of the remaining four varieties such as Pan, Ghulab Khas, Lahotia, and Kachnalwala was found in close proximity. The current study singled out three unique fingerprints amongst the seventy SSRs applied. Baganpali cultivar contained unique fingerprints against primer MiKVR_a010; Gulab khas cultivar showcased unique characters against primer MiKVR_a052 and another distinguished fingerprint was observed in mango cv Baganpali against primerMiKVR_a109. It was an indication that these varieties possessed specific genotypic behavior against applied markers. It is concluded that these varieties can play a significant role in improving the genetic makeup of mango varieties and can divulge useful features for varietal selection in a breeding program.
Sequencing by Avidity Enables High Accuracy With Low Reagent Consumption
Authors: Arslan Sinan , Kellinger Matthew , Kruglyak Semyon , LeVieux Jake , Mah Adeline , Wang Haosen , Zhao Junhua , Zhou Chunhong , Bailey John , Byrne Matthew , Chang Chiting , Chen Steve , Dennler Claudia , Dennler Samantha , Dien Vivian , Fuller Derek , Garcia Francisco , Guo Minghao , Kelley Ryan , Khandan Omid , Klein Michael , Kim Michael , Lin Bill , Liu Yu , Lopez Tyler , Mains Peter , Price Andrew , Taylor Hermes , Tippana Ramreddy , Tomaney Austin , Zhang Richard , Abtahi Minna , Ambroso Mark , Bajari Rosi , Bellizi Ava , Benitez Chris , Berard Daniel , Berti Lorenzo , Blease Kelly , Blum Angela , Boddicker Andrew , Bondar Leo , Bui Chris , Cappa Kevin , Chan Joshua , Chang Victor , Charov Katia , Chen Xiyi , Constandse Rodger , Damron Weston , Dawood Mariam , Debruno Nicole , Dmalanta John , Edoli Laure , Elango Keerthi , Faustino Nikka , Feng Chao , Ferrari Matthew , Frankie Kieth , Fries Adam , Galloway Anne , Gavrila Vlad , Gemmen Gregory , Ghadiali James , Goddard Logan , Roginski Adriana , Hendricks Garren , Hentschel Jendrick , Hseih Doris , Hwang-fu Yu-Hsein , Im Scott , Jin Chaoyi , Kincade Daniel , Lajooie Bryan , Levy Shawn , Li Yu , Liang Vincent , Light William , Lipsher Jonathan , Liu Tsungli , Long Guixia , Ma Rui , Mailloux Jack , Mandla Kyle , Martinez Anyssa , Mass Max , Meron Michael , Moh Celyne , Moore Rachel , Moreno Juan , Neysmith Jordan , Niman Cassandra , Nunez Jesus , Ojeda Micah , Owens Jenna , Ortiz Sara Espinosa , Piland Geoffrey , Chen Steve , Proctor Dan , Purba Josua , Ray Michael , Rong Daisong , Saade Virginia , Saha Sanchari , Sirajudeen Luqmanal , Stengel Gudrun , Stinson Ryan , Stone Michael , Sundseth Keoni , Thai Eileen , Thompson Connor , Tomas Gustav Santo , Trejo Christy , Trieger Greg , Truong Diane , Tse Ben , Voiles Benjamin , Vuong Henry , Wong Jennifer , Wu Chiung-Ting , Yu Hua , Yu Ming , Zhang Cindy , Zhao Da , Zheng Frank , He Molly , Previte Michael 
Published: November 2022

Abstract
We present a novel sequencing chemistry implemented as part of the AVITI system. Relying on the proximal DNA binding sites created through DNA amplification on a solid support, avidity sequencing uses multivalent nucleotide ligands on dye-labeled cores to simultaneously form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets. These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides by 100x and yield a negligible dissociation rate. We demonstrate the use of avidites within a novel sequencing technology that surpasses Q40 accuracy and enables a diversity of applications that include single cell RNA-seq and whole human genome sequencing.
A Review of the Effectiveness of the Integrated Solid Waste Management System in Institutional Solid Waste Management in Zimbabwe
Authors: SHABANI TAKUNDA , JERIE STEVEN 
Published: November 2022

Abstract
Abstract
The review focuses on the effectiveness of the integrated institutional solid waste management system in Zimbabwe, utilising already published literature. Zimbabwean institutional solid waste is produced from schools, tertiary learning institutions, barracks, prisons, police camps and medical facilities. Institutional solid waste consist of plastics, papers, metals, textiles, rubber, food waste, glass and wood. Construction and demolition waste such as broken bricks, concrete, sand, stones are also produced from institutions. Medical institutions are sources of sharps, pathological, infectious, pharmaceutical, radioactive and cytotoxic solid waste. Institutions also discard electric and electronic waste such as computers and cell phones. Institutional solid waste management approaches in Zimbabwe are characterised by illegal dumping, co-storage of waste, inefficiency collection and transportation as well as co-disposal in poorly constructed landfills, open pits and incinerators. However, open combustion and burying of solid waste is done at onsite to minimise environmental eyesore. These strategies cause water, land and air pollution while flora and fauna are not spared. People are affected by gastrointestinal diseases, work related risks and psychological problems. These problems are fuelled by management approaches and legal framework which neglect integrated institutional solid waste management. However, integrated approach have potential to reduce environmental health problems, accelerate economic development and support social life of Zimbabweans. Therefore, integrated model which consist of various stages and processes of institutional solid waste management, numerous aspects and all stakeholders was developed.
WITHDRAWN: Healthcare Resource Utilization and Costs in Patients With a Newly Confirmed Diagnosis of Lupus Nephritis in the United States Over a 5-year Follow-up Period
Authors: Bell Christopher F , Wu Benjamin , Huang Shirley P , Rubin Bernard , Averell Carlyne M , Chastek Benjamin , Hulbert Erin M 
Published: November 2022

Abstract
Background: Evaluate healthcare resource utilization (HCRU) and healthcare costs in patients with a newly confirmed diagnosis of lupus nephritis (LN) in the United States, over a 5-year follow-up period.
Methods: This retrospective, longitudinal cohort study (GSK Study 214102) utilized administrative claims data to identify individuals with a newly confirmed diagnosis of LN between August 1, 2011, and July 31, 2018, based on LN-specific International Classification of Diseases diagnosis codes. Index was the date of first claim with an LN-related diagnosis code during this period. HCRU, healthcare costs, and incidence of systemic lupus erythematosus (SLE) flares were reported for each post-index year of follow-up for eligible patients with at least 5 years continuous enrollment post-index.
Results: Of 2,159 patients with a newly confirmed diagnosis of LN meeting inclusion and exclusion criteria, 335 had at least 5 years continuous enrollment post-index.
HCRU was greatest in the first year post-LN diagnosis across all categories (inpatient admission, emergency room [ER] visits, ambulatory visits, and pharmacy use), and trended lower, though remained substantial, in the 5-year follow-up period. Among patients with HCRU, the mean (standard deviation [SD]) number of ER visits and inpatient admissions were 3.7 (4.6) and 1.8 (1.5), respectively, in Year 1, which generally remained stable in Years 2–5; the mean (SD) number of ambulatory visits and pharmacy fills were 35.8 (25.1) and 62.9 (43.8), respectively, in Year 1, and remained similar for Years 2–5.
Most patients (≥91.6%) had ≥1 SLE flare in each of the 5 years of follow-up. The proportion of patients who experienced a severe SLE flare was higher in Year 1 (31.6%) than subsequent years (14.3%–18.5%).
Total costs (medical and pharmacy; mean [SD]) were higher in Year 1 ($44,205 [71,532]) than subsequent years ($29,444 [52,310]–$32,222 [58,216]), driven mainly by inpatient admissions (Year 1: $21,181 [58,886]; subsequent years: $7,406 [23,331]–$9,389 [29,283]).
Conclusion: Patients with a newly confirmed diagnosis of LN have substantial HCRU and healthcare costs, particularly in the year post-diagnosis, and largely driven by inpatient costs. This highlights the need for interventions to prevent renal damage and improve outcomes in patients with renal involvement.
Bioprospecting of Indigenous Microalgae to Evaluate Their Potential for Bioenergy and Wastewater Treatment
Authors: Shafique Tahira , Safir Waqas , Shahid Mariam , uddin Nasir , Safir Anis , Khan Fahim Ullah 
Published: November 2022

Abstract
In this study we investigated the phycoremediation ability of indigenous microalgal strains using synthetic wastewater along with the production of high-value biomass. Two algal strains were collected, cultured, purified, and later named as named as BERC3 and BERC4 after morphologically and genetically identification. Nutrient concentrations, pH, and light intensity were optimized for maximum growth and metabolite content. Harvested biomass was characterized to check the impact of wastewater on the biomass productivity and metabolite content (lipids, carbohydrates, proteins, pigments) of selected strains. Results shows that both strains produced maximum biomass in low-light (150µmol m− 2 s− 1) at neutral to slightly alkaline pH. Both the strains performed very well in MGB11 (synthetic wastewater), indicated that these strains are suitable for growth in wastewater as those conditions favor biomass and metabolite production.
TLR4 inhibited autophagy by modulating PI3K/AKT/mTOR signaling pathway in GC cell lines
Authors: Zhang Qian , Dan Jun , Meng Shuang , Li Yingjie , Li Jing 
Published: November 2022

Abstract
Toll-like receptors (TLRs) are pattern recognition receptors that are found on both immune cells and cancerous cells. Gastric cancer (GC) cells/tissues have been shown to exhibit elevated levels of TLR4. Here, we examined the role of TLR4 on autophagy and growth in GC cells. Real-time quantitative PCR (RT-qPCR) and western blot (WB) were used to determine TLR4 levels at different stages of GC cells/tissues as well as the levels of autophagy-related proteins (ARPs) and determine the underlying signaling mechanism. Cellular growth was assessed via the CCK-8 assay. The protein and mRNA levels of ARPs were elucidated, followed by the estimation of the involved signaling pathways. Our results demonstrated that the modulation of the PI3K/AKT/mTOR pathway resulted from autophagy inhibition/induction, which was in turn induced by the overexpression and knockdown of TLR4. Thus, TLR4 played a vital role in GC progression.
Influence of Titania–graphene nanocomposite and coronatine on taxanes production and expression patterns of the key genes involved in taxol biosynthetic pathway in cell suspension culture of Taxus baccata
Authors: gougeh rahimeh - hemmati , Fatahi Reza - , shokrpour majid - , naghavi Mohammad Reza , Varshochi Alidad - , Mirjalili Mohammad Hossein , Nasiri Jaber - 
Published: November 2022

Abstract
In this study, the potential effects of two elicitors of coronatine and TiO2/graphene nanocomposite were evaluated on the expression of txs, dbat, bapt and dbtnbt genes, as well as taxanes production in cell suspension cultures of Taxus baccata. For this purpose, we studied the influence of 10 µMl− 1 of coronatine and 30 µgl− 1 of TiO2/graphene nanocomposite along with phenylalanine and benzoeic acid at the concentrations of 0.003 mM and 1.0 mM, respectively. Our results indicated that the amounts of taxanes induced by TiO2/graphene nanocomposite were upper than coronatine and control (42.47, 22.187, and 13.36 µgl− 1 DW). The maximum amounts of baccatin III and taxol were detected at four days after treatment with TiO2/graphene nanocomposite. The highest quantities of 10-deacetylbaccatin III and 10-deacetyltaxol were detected in day 16 after treatment with TiO2/graphene nanocomposite and the control, respectively. The amount of cephalomannine get raised gradually with TiO2/graphene nanocomposite. Meanwhile, TiO2/graphene nanocomposite increased the relative gene expressions TXS and DBTNBT than Coronatine. The uppermost amount of TXS expression was observed 4 days after application of two elicitors. The DBAT gene exhibited the lowest gene expression among the four genes studied. The minimum and the maximum level of BAPT expression was detected after treatment with coronatine and TiO2/graphene nanocomposite at day 4, respectively. The highest expression of DBTNBT occurred after treatment with TiO2/graphene nanocomposite at day 2, whilst treatment with coronatine had no effect. In conclusion, TiO2/graphene nanocomposite was more effective elicitors to induce production of taxanes and gene expressions levels during experiments as compared with coronatine and control.
Evaluation of a Novel Precision Care Model for Management of Chronic Disease in Primary Care: An Open Label Retrospective Analysis
Authors: Mallin Michael P. , Hall Jane , Herlihy Maria , Gelman Eduard J. , Stone Michael B. 
Published: November 2022

Abstract
Precision medicine is a relatively new field, based on the hypothesis that genetic predispositions influence an individual’s response to specific interventions such as diet, exercise, supplements and prescription medications. Despite the increase in commercially available genomic testing, few studies have investigated the effect of a physician-directed program to optimize health using genomics-based precision medicine.
We performed an observational cohort study to evaluate the effect of the Wild Health program, a physician and health coach service offering genomics-based lifestyle interventions and supplement recommendations, on biomarkers indicative of chronic disease.
Patients underwent genomic testing, biomarker testing, and a full evaluation by physicians and health coaches, with ongoing health coaching after the initial medical consultation. Improvements in several clinically relevant out-of-range biomarkers at baseline were identified in patients treated through lifestyle intervention without the use of prescription medication. Notably, normalization of several out-of-range biomarkers associated with chronic disease occurred in 47.5% (hemoglobin A1c [HbA1c]), 33.3% (low density lipoprotein particle number [LDL-P] , and 33.2% (C-reactive protein [CRP]).
Evolutionary ethnobiology and knowledge about medicinal resources used to treat COVID-19 symptoms in Salvaterra, Marajó, Pará, Brazil
Authors: Andrade Marcia Tatiana Vilhena Segtowich , Monteiro Samuele Mougo , Costa Vitoria Menezes da , de Assis Davison Marcio Silva , Tavares-Martins Ana Claudia Caldeira 
Published: November 2022

Abstract

The emergence of the COVID-19 pandemic has exerted selective pressure on local medical systems, encouraging human groups to seek alternative treatments, including traditional medicine. Natural treatments are critical alternatives for Amazonian populations due to the barriers they face to access health care services. Thus, this research aimed to carry out an ethnobiological study with evolutionary and ethnopharmacological approach on the selection processes and routes of knowledge transmission about treatments for COVID-19 to understand how local medical systems in the municipality de Salvaterra are adapting to the presence of COVID-19. Data collection was carried out through semi-structured interviews applied to residents of Salvaterra who tested positive for COVID-19. The interviews were conducted between July and October 2021. Sixty-two people, 31 living in urban and 31 in rural areas, were interviewed. Most respondents (74%) used natural treatments to treat the symptoms of COVID-19. Thirty-four plant species belonging to 21 botanical families were cited. Lemon [Citrus × limon(L.) Osbeck], garlic (Allium sativumL.), and jambu [Acmella oleracea(L.) R.K. Jansen] were the most cited. The preference for using leaves and plants from managed environments in preparing herbal remedies reinforces theories that the environment can influence how people use natural resources in each biome. The prevalence of horizontal (37%) instead of vertical (30%) transmission of knowledge reinforces that more diffuse ways, such as the horizontal and diagonal, are favored in unstable environments such as those created when a new disease emerges. According to the data, age and familiarity were the most substantial biases influencing the learning of biocultural traits in the studied local medical system.
Genetic testing and diagnostic strategies of fetal skeletal dysplasia: a preliminary study in Wu Han, China ​
Authors: Liu Wanlu , Cao Jing , Shi Xinwei , Li Yuqi , Qiao Fuyuan , Wu Yuanyuan 
Published: November 2022

Abstract
Background
Fetal skeletal dysplasia (FSD) is a diverse group of degenerative diseases of bone and cartilage disorders that can lead to movement disorder and even death. This study aims to deliver prenatal diagnosis through sonographic examination and genetic testing.
Methods
From September 2015 to April 2021, the study investigated 24 cases with suspected short-limb fetuses, which were obtained from Tongji Hospital affiliated to Tongji Medical College of Huazhong University of Science and Technology. To identify the causative gene, multiple approaches (including karyotype analysis, copy number variations and whole exome sequencing) were performed on these fetuses. And further segregation analysis of the candidate variant was performed in parents by using Sanger sequencing.
Results
① Out of 24 cases, likely pathogenic gene variants in FGFR3, FBN2, COL1A2, CUL7 and DYNC2H1 were detected for 6 cases; genetic variants in FGFR3, IMPAD1 and GORAB as possibly lethal mutations were identified in other 6 cases; and gene variants in WNT1, FBN1, OBSL1, COL1A1, DYNC2H1 and NEK1, known as Variant of Undetermined Significance (VUS), were found in 4 cases. The rest 8 cases showed undetectable mutation in the whole exome sequencing (WES) analysis. ②A genetic diagnosis determined 12 different skeletal dysplasia genotypes in 14/24 (58.3%) cases. The other 10 cases with wild type gene (41.7%) were normal and well developed in one-year follow-up survey after study.
Conclusion
Genetic testing combining with ultrasound scanning enhances the accurate diagnosis of fatal skeletal dysplasia in utero, and then provides appropriate genetic counseling.
Are banking &amp; financial institutions ready for the transformation? An analysis of FinTech adoption challenges using DEMATEL
Authors: Mathur Pooja , Gupta Neha , Agarwal Mohini 
Published: November 2022

Abstract
This study aims to analyse the various challenges faced by the financial institutions in the adoption of financial technologies. Forty challenges are identified through extensive literature review and expert’s opinions which are further classified into four main categories using EFA. Finally, the identified and validated challenges are evaluated for causal relationship using DEMATEL technique. The results of the study showed that challenges like perceived value of customers, handling of big data, banking regulations, and money laundering risk are the most influential challenges. The managers and policymakers need to focus on these challenges which will ultimately leads to other challenges as well.
Dietary of astaxanthin derived from a metabolic-engineered yeast to improve red-color performance, immune response and resistance to Vibrio parahaemolyticus on white shrimp Penaeus vannamei.
Authors: Lin Yu-Ju , Chang Jui-Jen , Huang Huai-Ting , Lee Chih-Ping , Hu Yeh-Fang , Wu Mao-Lun , Nan Fan-Hua , Huang Chih-Yang 
Published: November 2022

Abstract
Astaxanthin (AST), a super antioxidant with coloring and medical properties, renders it a beneficial feed additive for shrimp. This study conducted a white shrimp feeding trial of 3S, 3’S isoform AST, which was derived from metabolic-engineered Kluyveromyces marxianus fermented broth (TB) and its extract (TE) compared to sources from two chemically synthetic ASTs (Carophyll Pink [CP] and Lucantin Pink [LP]), which contain 3S, 3’S, 3R, 3’S (3S, 3’R) and 3R, 3’R isoforms ratio of 1:2:1. The effects on red coloration, immune parameters and resistance to Vibrio infection were evaluated. Four AST sources were incorporated into the diets at concentrations of 0 (control), 100 mg kg− 1 (TB100, TE100, CP100, and LP100), and 200 mg kg− 1 (TB200, TE200, CP200, and LP200). Results revealed that in week 4, shrimps that received AST-supplemented feeds, especially TB100, TB200, and TE200, significantly increased redness (a*) values. Immune responses including phagocytosis activity, superoxide-anion production, phenoloxidase activity, and immune-related genes were examined on days 0, 1, 2, 4, 7, 14, 21, and 28. Generally, shrimps that received AST-supplemented feeds exhibited higher immune responses on days 7 and 14 than the control feed. Gene expression levels of superoxide dismutase and glutathione peroxidase were significantly upregulated on days 7 and 14 in shrimps that received AST-supplemented feeds, while genes of penaeidins, antilipopolysaccharide factor, and lysozyme were upregulated on days 4, 7, and 14, especially received TB200 and TE200. Furthermore, shrimps that received TB100, TE100, CP100, and LP100 7 days were then challenged with Vibrio parahaemolyticus and the result demonstrated higher survival rates especially TB100 at 168 h than the control feed. In conclusion, incorporating AST into the diets enhanced shrimp red coloration, immune parameters, and resistance against V. parahaemolyticus infection. The K. marxianus-derived AST exhibited higher performance than did chemical AST to be a potential feed additive in shrimp aquaculture.