bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perfil de susceptibilidade das bactérias na microbacia do Rio Branco, Roraima, extremo Norte da Amazônia
Authors: Farias Leovergildo Rodrigues , Vital Marcos Jose Salgado , Correa Ana Paula Folmer , Riss Jordana Souza Paula , Tormente Fabiana Vieira 
Published: June 2022

A qualidade dos recursos hídricos se constituiu em uma categoria primordial de preocupação para os pesquisadores, pois os impactos ambientais ocasionados pelo crescimento acelerado e desordenado das cidades torna a degradação desses recursos intensa e rapidamente perceptível, inclusive no extremo norte da amazônia. Nesse contexto, o objetivo desse estudo foi avaliar o perfil de susceptibilidades das bactérias isoladas da microbacia do Rio Branco, Roraima, extremo norte da Amazônia. Realizou-se o perfil de susceptibilidade dos isolados bacterianos empregando-se oito discos de antibacterianos e na identificação dos isolados empregou-se o método de Matrix Assisted Laser Desorption/Ionization – Time ofFlight Mass Spectrocopy (MALDI-TOF/MS). Os resultados obtidos demonstaram diferença significativa entre os períodos seco e chuvoso em relação à qualidade físico-química e microbiológica da água nos diferentes pontos coletados na microbacia do Rio Branco,RR. Do total de isolados analisados, apenas 31 % das bactérias, apresentaram resistência aos antibacterianos amicacina, amoxicilina, azitromicina e ciprofloxacina, evidenciando que os antibacterianos clorafenicol, gentamicina, imipenem e meropenem apresentaram eficiência de 100 % sobre os isolados. Foram identificadas as espécieis Klebsiella pneumoniae, Chromobacterium violaceum, Escherichia coli, Enterobacter kobei, Bacillus pumilus, Salmonella typhi e do gênero Staphylococcus sp. em ambos os períodos (seco e chuvoso) de coletas avaliados, sendo constatado a presença de bactérias consideradas potencialmente patogênicas, como Klebsiella pneumoniae, Staphylococcus sp., Enterococcus faecalis, Escherichia coli, Salmonella typhi e Shigella sp., tornando necessário a continuidade de estudos que visem identificar e avaliar o perfil de susceptibilidade das bactérias presentes em outros rios e igarapés do estado de Roraima.
The LINC00261/MiR105-5p/SELL axis is involved in dysfunction of B cell and is associated with overall survival in hepatocellular carcinoma
Authors: Song Hao , Huang Xing-Feng , Hu Shu-yang , Lu Lei-Lei , Yang Xiao-Yu 
Published: June 2022

Background
Previous studies have been reported the immune dysfunction of various live tissues. However, the potential molecular mechanism of post-transcriptional regulation of immune related genes in hepatocellular carcinoma (HCC) is still not clear. We tried to identify crucial immune related biomarkers associated with HCC patients’ outcomes and to reveal the transcriptional regulation.

Method
The fractions of 22 immune cells in tumor and adjacent tissues were estimated by CIBERSORT. Kruskal-Wallis test and differentially expressed analyzes were used for comparative studies. Cox proportional hazard regression model, Kaplan-Meier estimates and Log-rank test were used for survival analyses.

Results
From The Cancer Genome Atlas (TCGA), the gene, lncRNA and miRNA expression profiles of 379 HCC samples with clinical information were used for comparative studies. Eleven adaptive and innate immune cell types were significantly altered in HCC samples, including B cell memory, regulatory T cells and follicular helper T cells. Differentially expressed competing endogenous RNA (ceRNA) network associated with patients’ overall survival was identified. Then, the novel pathway, including LINC00261, MiR105-5p and selectin L(SELL) was found and may be potential novel biomarkers for patients’ outcomes and immunotherapy. Furthermore, SELL was significantly positively correlated (correlation coefficients: 0.47–0.69) with 12 known gene signatures of immunotherapy except for programmed cell death 1 (PDCD1).

Conclusions
Our findings could provide insights into the selection of novel LINC00261/MiR105-5p/SELL pathway which is associated with overall survival and may impact on efficacy of immunotherapy in HCC.
DESIGUALDADE GLOBAL, CRISE MULTIDIMENSIONAL E AS FALÁCIAS DO DESENVOLVIMENTO
Authors: Costa Lima Marcos , Spellmann Samuel 
Published: June 2022

O capitalismo contemporâneo tem ampliado sua condição produtora de desigualdades. Este aprofundamento tem sido revelado por meio do caráter multidimensional tomado pela crise capitalista. A continuidade da crise capitalista também desafia as noções ligadas às possibilidades de correção do capitalismo e a superação das desigualdades inerentes ao modo de produção pela figura histórica da reforma. Este artigo objetiva evidenciar processos específicos, mas globais, assinalando suas “impossibilidades sistêmicas”, que apontam criticamente para as falácias do desenvolvimento, em projetos que aprofundam as disparidades sociais de toda ordem, ao serviço do grande capital e em detrimento dos trabalhadores. Isso é feito com a exposição de dois estudos de caso, Brasil e China, evidenciando o caráter multidimensional da crise capitalista e sua relação com a produção de desigualdades.
Trichodesmium
Authors: Webb Eric A , Foster Rachel A , Villareal Tracy , Waterbury John B , Zehr Jonathan P 
Published: June 2022

AbstractTri.cho.des'mi.um. Gr. fem. n.thrix,trichos, hair; Gr. masc. n.desmos, bond; N.L. neut. n.Trichodesmium, bonded filaments.Cyanobacteria / Subsection III / Oscillatoriales / Microcoleaceae / TrichodesmiumFilamentous, marine cyanobacteria that divide exclusively by binary fission in one plane. Trichomes of different species or strains range from 5 to 35 μm in diameter. Isolates have cells that range from barrel to disk shaped; constrictions at cross‐walls are shallow. Trichomes can arrange in parallel fascicles or form radiate macroscopic clustersin situ(i.e., colonies), while many strains and natural communities exist as free trichomes. Gliding motility has been observed.Trichodesmiumspecies are often important N2‐fixing constituents of the plankton in tropical to subtropical oceans. Additionally, they can form extensive, ephemeral surface blooms with high cellular abundance under calm sea conditions.Trichodesmiumspp. are aerobic, oxygenic phototrophs that fix carbon dioxide using the RuBisCO pathway. Photoheterotrophy has not been demonstrated. N2fixation is performed during the daylight hours without heterocysts, and neither hormogonia nor akinetes have been observed.Trichodesmiumisolates range from reddish/orange to greenish yellow in color, with absorption peaks for phycourobilin (PUB, 498 nm), phycoerythrobilin (PEB, 545 nm), and/or phycocyanin (PC, 618 nm). All known members contain abundant gas vacuoles, generally dispersed throughout the cells and exceptionally resistant to collapse, even at hydrostatic pressures of 12–27 bars.DNA G + C content (mol%) = 33–34.Genome of type species:Trichodesmium erythraeumIMS101 (Walworth et al., 2015).Type species:Trichodesmium erythraeumEhrenberg ex Gomont 1892.
New phenotype of RTN2‐related spectrum: Complicated form of spastic paraplegia‐12
Authors: Tian Wotu , Zheng Haoran , Zhu Zeyu , Zhang Chao , Luan Xinghua , Cao Li 
Published: June 2022

AbstractObjectiveSpastic paraplegia‐12 (SPG12) is a subtype of hereditary spastic paraplegia caused by Reticulon‐2 (RTN2) mutations. We described the clinical and genetic features of three SPG12 patients, functionally explored the potential pathogenic mechanism of RTN2 mutations, and reviewed RTN2‐related cases worldwide.MethodsThe three patients were 31, 36, and 50 years old, respectively, with chronic progressive lower limb spasticity and walking difficulty. Physical examination showed elevated muscle tone, hyperreflexia and Babinski signs in the lower limbs. Patients 1 and 3 additionally had visual, urinary, and/or coordination dysfunctions. Patient 2 also had epileptic seizures. RTN2 mutations were identified by whole‐exome sequencing, followed by Sanger sequencing, segregation analysis, and phenotypic reevaluation. Functional examination of identified mutations was further explored.ResultsThree variants in RTN2 were identified in Patient 1 (c.103C>T, p.R35X), Patient 2 (c.230G>A, p.G77D), and Patient 3 (c.337C>A, p.P113T) with SPG, respectively. Western blotting revealed the p.R35X with smaller molecular weight than WT and other two missense mutants. Immunostaining showed the wild type colocalized with endoplasmic reticulum (ER) in vitro. p.R35X mutant diffusely distributes in the cytoplasm, losing colocalization with ER. p.G77D and p.P113T co‐localized with ER, which was abnormally aggregated in clumps.InterpretationIn this study, we identified three cases with complicated SPG12 due to three novel RTN2 mutations, respectively, presenting various phenotypes: classic SPG symptoms with (1) visual abnormalities and sphincter disturbances or (2) seizures. The phenotypic heterogeneity might arise from the abnormal subcellular localization of mutant Reticulon‐2 and improper ER morphogenesis, revealing the RTN2‐related spectrum is still expanding.
Phage‐Derived Oncolytic Viruses with 3C from Seneca Valley Virus for Targeted Therapy of Cervical Cancer
Authors: Liu Tingting , Zhao Ningwei , Shi Mingze , Shen Yuanzhao , Mao Chuanbin , Zhou Xin 
Published: June 2022

AbstractCancer gene therapy based on various gene delivery vectors has some potential but also has obvious disadvantages. In this study, a new M13 phage‐based oncolytic virus is constructed that carried the RGD peptides to target tumor cells and the 3C gene of Seneca Valley virus (SVV) preceded by a eukaryotic initial transcriptional region (ITR) to transcribe an oncolytic protein to kill tumor cells. Recombinant virus particles of 1200 nm in length are obtained in large quantities by transfecting the recombinant M13 phage plasmid into the host BL2738 and are investigated in vitro in tumor cells and in vivo in tumor‐bearing mice to evaluate their antitumor effect. The experiments using Hela cells confirm that the engineered M13 phage can target and enter Hela cells, and express the SVV 3C protein, resulting in apoptosis of target cells by upregulating the expression of caspase 3. Furthermore, the results of experiments in vivo also show that the recombinant phage significantly inhibits the enhanced tumor volume in nude mice compared to the control groups. The M13 phage may be engineered to fuse with a variety of oncolytic proteins to inhibit the growth of tumor cells in the future, providing a promising phage‐based targeted oncolytic reagent.
Growth rate and life history shape plant resistance to herbivores
Authors: Jacobsen Deidra J. 
Published: June 2022

AbstractPremisePlant defenses are shaped by many factors, including herbivory, lifespan, and mating system. Predictions about plant defense and resistance are often based on resource allocation trade‐offs with plant growth and reproduction. Additionally, two types of plant resistance, constitutive and induced resistance, are predicted to be evolutionary alternatives or redundant strategies. Given the variety of plant trait combinations and non‐mutually exclusive predictions, examining resistance strategies in related species with different combinations of growth and reproductive traits is important to tease apart roles of plant traits and evolutionary history on plant resistance.MethodsPhylogenetic comparative methods were used to examine the potentially interacting influences of life history (annual/perennial), mating system (self‐compatible/self‐incompatible), and species growth rates on constitutive resistance and inducibility (additional resistance following damage) across Physalis species (Solanaceae).ResultsResistance was evolutionarily labile, and there was no correlation between constitutive resistance and inducibility. Annual species with fast growth rates displayed higher constitutive resistance, but growth rate did not affect constitutive resistance in perennials. In contrast, inducibility was negatively associated with species growth rate regardless of life history or mating system.ConclusionsThe different effects of plant life history and growth rate on constitutive resistance and inducibility indicate that defensive evolution is unconstrained by a trade‐off between resistance types. The interactions among plant life history, growth, and herbivore resistance show that plant defense is shaped not only by herbivore environment, but also by plant traits that reflect a plant's evolutionary history and local selective pressures.
Disparities and outcomes of patients living with Down Syndrome undergoing healthcare transitions from pediatric to adult care: A scoping review
Authors: Varshney Karan , Iriowen Rosemary , Morrell Kayla , Pillay Preshon , Fossi Alexander , Stephens Mary M. 
Published: June 2022

AbstractDown Syndrome (DS) is one of the most common chromosomal disorders worldwide, and people with DS experience more co‐morbidities and have poorer health outcomes compared to the general population. An area that is not well understood is how patients with DS transition from pediatric to adult care, as well as the details, barriers, and difficulties of these transitions for patients. Hence, we aimed to provide a scoping review of the literature in PubMed, Scopus, and CINAHL on the topic of healthcare transitions (HCTs) for patients with DS. Findings suggest patients with DS who continued receiving care as an adult from a pediatric care provider tended to experience co‐morbidities and other adverse health issues at higher rates than those who entirely switch to an adult‐care team. Patients with DS were unable to undergo transition due to multiple barriers, such as low income, limited/public insurance, gender, and race. We propose potential steps for transition, which focus on ensuring early planning, communicating better, coordinating services, assessing decision‐making capacity, and providing ongoing social and financial support. Future research must further identify and address barriers to HCTs for people with DS.
Broadening the phenotypic spectrum of TUBA1A tubulinopathy to syndromic arthrogryposis multiplex congenita
Authors: Weber Mathilde , Jaber Dana , Encha‐Razavi Ferechte , Julien Emmanuel , Grevoul‐Fesquet Julie , Steffann Julie , Melki Judith , Martinovic Jelena 
Published: June 2022

AbstractThe recent finding that some patients with fetal akinesia deformation sequence (FADS) carry variants in the TUBB2B gene has prompted us to add to the existing literature a first description of two fetal FADS cases carrying TUBA1A variants. Hitherto, only isolated cortical malformations have been described with TUBA1A mutation, including microlissencephaly, lissencephaly, central pachygyria and polymicrogyria‐like cortical dysplasia, generalized polymicrogyria cortical dysplasia, and/or the “simplified” gyral pattern. The neuropathology of our fetal cases shows several common features of tubulinopathies, in particular, the dysmorphism of the basal ganglia, as the most pathognomonic sign. The cortical ribbon anomalies were extremely severe and concordant with the complex cortical malformation. In conclusion, we broaden the phenotypic spectrum of TUBA1A variants, to include FADS.
Borylation Directed Borylation of Indoles Using Pyrazabole Electrophiles: A One‐Pot Route to C7‐Borylated‐Indolines
Authors: Pahl Jürgen , Noone Emily , Uzelac Marina , Yuan Kang , Ingleson Michael J. 
Published: June 2022

AbstractPyrazabole (1) is a readily accessible diboron compound that can be transformed into ditopic electrophiles. In 1 (and derivatives), the B⋅⋅⋅B separation is ca. 3 Å, appropriate for one boron centre bonding to N and one to the C7 of indoles and indolines. This suitable B⋅⋅⋅B separation enables double E−H (E=N/C) functionalisation of indoles and indolines. Specifically, the activation of 1 with HNTf2 generates an electrophile that transforms N−H indoles and indolines into N/C7‐diborylated indolines, with N−H borylation directing subsequent C7−H borylation. Indole reduction to indoline occurs before C−H borylation and our studies indicate this proceeds via hydroboration—C3‐protodeboronation to produce an intermediate that then undergoes C7 borylation. The borylated products can be converted in situ into C7‐BPin‐N‐H‐indolines. Overall, this represents a transient directed C−H borylation to form useful C7‐BPin‐indolines.
Ultra‐High Temperature Molten Oxide Electrochemistry
Authors: Wang Mingyong , Jiao Handong , Pu Zhenghao , Hong Biao , Ge Jianbang , Xiao Wei , Jiao Shuqiang 
Published: June 2022

AbstractRecently, the ultra‐high temperature electrochemistry (UTE, about >1000 °C) has emerged, which represents an exploration to extend the temperature limit of human technology in electrochemical engineering. UTE has far‐reaching impact on revolutionary low‐carbon metal extraction and the in situ production of oxygen for deep‐space exploration. It is hence of urgency to systematically summarize the development of UTE. In this Review, the basic concepts of UTE and the physicochemical properties of molten oxides are analyzed. The principles in the design of inert anodes for the oxygen evolution reaction in molten oxides are discussed, which forms a solid basis for the in situ production of oxygen from simulated lunar regolith by UTE. Furthermore, liquid metal cathodes for revolutionary titanium extraction and ironmaking/steelmaking are highlighted. With emphasis on the key challenges and perspectives, the Review can provide valuable inspiration for the rapid advancement of UTE.
Thermally Activated and Aggregation‐Regulated Excitonic Coupling Enable Emissive High‐Lying Triplet Excitons**
Authors: Wang Tao , De Joydip , Wu Sen , Gupta Abhishek Kumar , Zysman‐Colman Eli 
Published: June 2022

AbstractRoom‐temperature phosphorescence (RTP) originating from higher‐lying triplet excitons remains a rather rarely documented occurrence for purely organic molecular systems. Here, we report two naphthalene‐based RTP luminophores whose phosphorescence emission is enabled by radiative decay of high‐lying triplet excitons. In contrast, upon cooling the dominant phosphorescence originates from the lowest‐lying triplet excited state, which is manifested by a red‐shifted emission. Photophysical and theoretical studies reveal that the unusual RTP results from thermally activated excitonic coupling between different conformations of the compounds. Aggregation‐regulated excitonic coupling is observed when increasing the doping concentration of the emitters in poly(methylmethacrylate) (PMMA). Further, the RTP quantum efficiency improves more than 80‐fold in 1,3‐bis(N‐carbazolyl)benzene (mCP) compared to that in PMMA. This design principle offers important insight into triplet excited state dynamics and has been exploited in afterglow‐indicating temperature sensing.
Producing high value unsaturated fatty acid by whole‐cell catalysis using microalga: A case study with Tribonema minus
Authors: Zhou Wenjun , Ji Xiaotong , Zheng Li , Yang Guanpin , Liu Tianzhong 
Published: June 2022

AbstractHigh value unsaturated fatty acids can be produced by de novo synthesis in microalgal cells, especially via heterotrophic cultivation. Unfortunately, the lipid accumulation of heterotrophic microalgae cannot be improved efficiently in conventional ways. Here we reported heterotrophic Tribonema minus, a promising resource for the production of palmitoleic acid which has increasing demands in health service for patients with metabolic syndrome, as whole‐cell biocatalyst to develop a novel way of shifting low value exogenous saturated fatty acids to high value ones. Results showed that myristic acid is the best precursor for whole‐cell catalysis; it elevated the lipid content of T. minus to 42.2%, the highest among the tried precursors. The influences of cultivation condition on the utilization of extrinsic myristic acid and lipid accumulation were also determined. Under the optimized condition, the lipid content reached as high as 48.9%. In addition, our findings showed that ~13.0% of C16:1 in T. minus is derived from extrinsic myristic acid, and 30.1% of metabolized precursor is converted into heterologous fatty acids. Thus, a feasible approach for both increasing the value of low value saturated fatty acid by bioconversion and enhancing the lipid accumulation in microalgae is proposed by supplementing extrinsic myristic acid.
Toward reanimating the laughter‐involved large‐scale brain networks to alleviate affective symptoms
Authors: Zarei Shahab A. , Yahyavi Seyedeh‐Saeedeh , Salehi Iman , Kazemiha Milad , Kamali Ali‐Mohammad , Nami Mohammad 
Published: June 2022

AbstractIntroductionThe practicality of the idea whether the laughter‐involved large‐scale brain networks can be stimulated to remediate affective symptoms, namely depression, has remained elusive.MethodsIn this study, 25 healthy individuals were tested through 21‐channel quantitative electroencephalography (qEEG) setup upon resting state and while submitted to standardized funny video clips (corated by two behavioral neuroscientists and a verified expert comedian, into neutral and mildly to highly funny). We evaluated the individuals’ facial expressions against the valence and intensity of each stimulus through the Nuldos face analysis software. The study also employed an eye‐tracking setup to examine fixations, gaze, and saccadic movements upon each task. In addition, changes in polygraphic parameters were monitored upon resting state and exposure to clips using the 4‐channel Nexus polygraphy setup.ResultsThe happy facial expression analysis, as a function of rated funny clips, showed a significant difference against neutral videos (p < 0.001). In terms of the polygraphic changes, heart rate variability and the trapezius muscle surface electromyography measures were significantly higher upon exposure to funny vs. neutral videos (p < 0.5). The average pupil size and fixation drifts were significantly higher and lower, respectively, upon exposure to funny videos (p < 0.01). The qEEG data revealed the highest current source density (CSD) for the alpha frequency band localized in the left frontotemporal network (FTN) upon exposure to funny clips. Additionally, left FTN acquired the highest value for theta coherence z‐score, while the beta CSD predominantly fell upon the salience network (SN).ConclusionsThese preliminary data support the notion that left FTN may be targeted as a cortical hub for noninvasive neuromodulation as a single or adjunct therapy in remediating affective disorders in the clinical setting. Further studies are needed to test the hypotheses derived from the present report.
Significant Effect of the Flexibility of Bridging Alkyl Chains on the Proximity of Stacked Porphyrin and Phthalocyanine Conjugated with a Fourfold Rotaxane Linkage
Authors: Yamada Yasuyuki , Iida Hayato , Shibano Shinya , Mihara Nozomi , Kato Tatsuhisa , Tanaka Kentaro 
Published: June 2022

AbstractInvited for the cover of this issue are Kentaro Tanaka at Nagoya University and co‐workers. The image depicts the importance of the flexibility of bridging alkyl chains in a molecular conjugate to pack the components closely to generate efficient molecular communication. Read the full text of the article at 10.1002/chem.202200819.