bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complex transcatheter left atrial appendage closure using a tailored trans-jugular approach simulated by 3D printing: a case report
Authors: Ciobotaru Vlad , Tadros Victor-Xavier , Martin Claire A , Hascoet Sebastien 
Published: July 2022

AbstractBackgroundTranscatheter left atrial appendage (LAA) closure (LAAc) is less feasible in patients with unusual LAA anatomy.Case summaryA 65-year-old woman with persistent atrial fibrillation was referred for LAAc. Transesophageal echocardiography (TEE) revealed spontaneous contrast in the LAA without formation of a thrombus; the LAA shape was tortuous and difficult to assess. A first LAAc procedure was unsuccessful given the unsuitable sheath position. Therefore, a soft three-dimensional (3D) model printing was performed by laser sintering and revealed excessive sheath kinking with an inferior approach, but successful deployment would be feasible using a superior approach. Successful trans-jugular implantation of a Watchman FLX 31 device in stable position without residual leakage was achieved during the subsequent procedure. At 3-month follow-up, and after cessation of oral anticoagulation, the patient’s symptoms improved. Imaging demonstrated complete LAA occlusion and correct placement of the device along the LAA superior axis.DiscussionThis is the first-reported clinical case of a complex transcatheter LAAc through a trans-jugular approach. Simulating the patient’s anatomy with a laser sintering 3D-printed model showed why the transfemoral approach failed, validated the trans-jugular procedure, enabled selection of the simple curve access sheath that had the most direct trajectory towards the LAA, confirmed that transseptal puncture was possible, allowed determination of the angle of puncture, enabled selection of the most appropriate LAA device and had a very low cost compared with planning software or other printing methods.
Endomyocardial fibrosis of the right ventricle in a patient with schistosomiasis: a case report
Authors: Espinoza Romero Cristhian , Lima Ivna Cunha Vieira , Hotta Viviane Tiemi , Bocchi Edimar Alcides , Salemi Vera Maria Cury 
Published: July 2022

Abstract

Background
Endomyocardial fibrosis (EMF) is a rare and underdiagnosed cause of restrictive cardiomyopathy. Its aetiology is not yet defined and could be caused by the influence of different clinical factors that seem to combine with genetic aspects of individuals susceptible to an inflammatory process that leads to formation of fibrosis.

Case summary
We describe a case of a 50-year-old man from the northeastern region of Brazil, where there is high prevalence of schistosomiasis. He presented to our centre with symptoms of right heart failure. The echocardiogram showed normal left ventricular ejection fraction. Right ventricular had normal systolic function but in the apical region was filled with a homogeneous and hypoechoic image causing obliteration and restriction of the apex. The late gadolinium enhancement with cardiac magnetic resonance showed diffuse and heterogeneous subendocardial fibrosis in the right ventricle apex consistent with EMF, but declined endocardiectomy.

Discussion
This report presents an interesting case of EMF and schistosomiasis simultaneously. The hypothesis of parasitosis as a probable cause of EMF was raised by helminth-induced hypereosinophilia. Complementary imaging tests such as magnetic resonance imaging and echocardiography, in addition to clinical and epidemiological suspicion, are essential for its diagnosis. Early surgical resolution becomes crucial for long-term survival.
Left-to-right rotated single lung transplant
Authors: Chida Masayuki , Inoue Takashi , Araki Osamu , Maeda Sumiko 
Published: July 2022

Abstract
We successfully performed left-to-right rotated single lung transplants in 2 patients. For this procedure, the left pulmonary artery of the donor undergoes a U-shaped turn with A3 at the bottom to anastomose with the recipient’s right pulmonary artery in front of the bronchus. It is extremely important to avoid kinking the pulmonary artery at the site of the U-shaped turn.
Regional variation in biomechanical properties of ascending thoracic aortic aneurysms
Authors: Salmasi M Yousuf , Sasidharan Sumesh , Frattolin Jennifer , Edgar Lowell , Stock Ulrich , Athanasiou Thanos , Moore Jr James 
Published: July 2022

Abstract

OBJECTIVES
This study aims to characterize the material properties of ascending thoracic aortic aneurysmal tissue, using regional biomechanical assessment of both tensile and dissection propagation peel strength.

METHODS
Thirty-four aneurysm specimens (proximal thoracic aorta) were harvested en-bloc from patients undergoing surgery for aneurysm replacement. Specimens were processed into regional samples of similar shapes covering the whole aneurysm isosurface, according to a structured protocol, in both orientations (longitudinal and circumferential). Thickness mapping, uniaxial tensile and peel tests were conducted, enabling calculation of the following parameters: true stress/strain, tangential modulus, tensile strength, peeling force and dissection energy. Two constitutive material models were used (hyperelastic models of Delfino and Ogden) to fit the data. A circumferential strip of tissue was also obtained for computational histology [regional quantification of (i) elastin, (ii) collagen and (iii) smooth muscle cells].

RESULTS
The aortic wall was thinner on the outer curve (2.21, standard deviation (SD) 0.4 mm vs inner curve 2.50, SD 0.12 mm). Advanced patient age and higher pulse wave velocity (externally measured) were predictors of increased aortic wall thickness. Tensile strength was higher in the circumferential versus longitudinal direction when analysed according to anatomical regions. Both peel force (35.5, 22 N/m) and dissection energy (88.5, 69 J/m2) were on average lowest at the outer curve of the aneurysm in the longitudinal orientation. Delfino and Ogden model constants varied throughout anatomical regions, with the outer curve being associated a higher ɑ constant (Delfino) and lower µ1 constant (Ogden) (P &lt; 0.05) indicating increased stiffness. Histologically, collagen abundance was significantly related to circumferential and longitudinal strength (P= 0.010), whilst smooth muscle cell count had no relation with any mechanical property (P &gt; 0.05).

CONCLUSIONS
Our results suggest that the outer aortic curve is more prone to dissection propagation and perhaps less prone to rupture than the inner aortic curve. This strengthens the notion of disease heterogeneity in ascending thoracic aortic aneurysms and has implications for the pathogenesis of aortic dissection.
Obesity and outcomes after left ventricular assist device implantation: insights from the EUROMACS Registry
Authors: Angleitner Philipp , Kaider Alexandra , De By Theo M M H , Dimitrov Kamen , Schlöglhofer Thomas , Tops Laurens F , Fiane Arnt E , Rábago Gregorio , Laufer Günther , Zimpfer Daniel 
Published: July 2022

Abstract

OBJECTIVES
The objective was to analyse associations between obesity and outcomes after left ventricular assist device (LVAD) implantation.

METHODS
A retrospective analysis of the EUROMACS Registry was performed. Adult patients undergoing primary implantation of a continuous-flow LVAD between 2006 and 2019 were included (Medtronic HeartWare® HVAD®, Abbott HeartMate II®, Abbott HeartMate 3™). Patients were classified into 4 different groups according to body mass index at the time of surgery (body mass index &lt;20 kg/m2: n = 254; 20–24.9 kg/m2: n = 1281; 25–29.9 kg/m2: n = 1238; ≥ 30 kg/m2: n = 691).

RESULTS
The study cohort was comprised of 3464 patients. Multivariable Cox proportional cause-specific hazards regression analysis demonstrated that obesity (body mass index ≥30 kg/m2) was independently associated with significantly increased risk of mortality (body mass index ≥30 vs 20–24.9 kg/m2: hazard ratio 1.36, 95% confidence interval 1.18–1.57, overall P &lt; 0.001). Moreover, obesity was associated with significantly increased risk of infection and driveline infection. The probability to undergo heart transplantation was significantly decreased in obese patients (body mass index ≥30 vs 20–24.9 kg/m2: hazard ratio 0.59, 95% confidence interval 0.48–0.74, overall P &lt; 0.001).

CONCLUSIONS
Obesity at the time of LVAD implantation is associated with significantly higher mortality and increased risk of infection as well as driveline infection. The probability to undergo heart transplantation is significantly decreased. These aspects should be considered when devising a treatment strategy before surgery.
Hypertension in children and adolescents
Authors: de Simone Giovanni , Mancusi Costantino , Hanssen Henner , Genovesi Simonetta , Lurbe Empar , Parati Gianfranco , Sendzikaite Skaiste , Valerio Giuliana , Di Bonito Procolo , Di Salvo Giovanni , Ferrini Marc , Leeson Paul , Moons Philip , Weismann Constance G , Williams Bryan 
Published: July 2022

Abstract
Definition and management of arterial hypertension in children and adolescents are uncertain, due to different positions of current guidelines. The European Society of Cardiology task-force, constituted by Associations and Councils with interest in arterial hypertension, has reviewed current literature and evidence, to produce a Consensus Document focused on aspects of hypertension in the age range of 6–16 years, including definition, methods of measurement of blood pressure, clinical evaluation, assessment of hypertension-mediated target organ damage, evaluation of possible vascular, renal and hormonal causes, assessment and management of concomitant risk factors with specific attention for obesity, and anti-hypertensive strategies, especially focused on life-style modifications. The Consensus Panel also suggests aspects that should be studied with high priority, including generation of multi-ethnic sex, age and height specific European normative tables, implementation of randomized clinical trials on different diagnostic and therapeutic aspects, and long-term cohort studies to link with adult cardiovascular risk. Finally, suggestions for the successful implementation of the contents of the present Consensus document are also given.
Geochemical and microbiological profiles in hydrothermal extreme acidic environments (Pisciarelli Spring, Campi Flegrei, Italy)
Authors: Crognale Simona , Venturi Stefania , Tassi Franco , Rossetti Simona , Cabassi Jacopo , Capecchiacci Francesco , Bicocchi Gabriele , Vaselli Orlando , Morrison Hilary G , Sogin Mitchell L , Fazi Stefano 
Published: July 2022

AbstractAlthough terrestrial hydrothermal systems are considered among the most fascinating environments, how their unique and extreme conditions can affect microorganisms selection and the role in biogeochemical cycles has not yet been well elucidated. A combined geochemical and microbiological exploration in waters and sediments from 10 sampling points along a sharp temperature gradient (15–90°C) within an extremely acidic hydrothermal system (Pisciarelli Spring, Campi Flegrei area, southern Italy) displayed how hydrothermal fluids influence the microbial dynamics. This area was characterized by high levels of reduced gaseous species (e.g. H2S, H2, CH4, CO) and very low pH values (&lt;2.3). Thermodynamic calculations revealed a high microbial catabolic potential in oxidation/reduction reactions of N-, S- and Fe-bearing species. Overall, an increase of the archaeal/bacterial abundance ratio was observed by decreasing temperature and pH values. In particular, Archaea and Bacteria were present in almost equal cell abundance (up to 1.1 × 109 and 9.3 × 108 cell/g, respectively) in the &lt;70°C sampling points (average pH = 2.09); on the contrary, the highest temperature waters (85–90°C; average pH = 2.26) were characterized by a low abundance of archaeal cells. The high-throughput sequencing of the 16S rRNA genes indicated strong differences in archaeal and bacterial communities composition along the temperature gradient. However, the microbiome in this extreme environment was mainly constituted by chemoautotrophic microorganisms that were likely involved in N-, S- and Fe-bearing species transformations (e.g. Acidianus infernus, Ferroplasma acidarmanus, Acidithiobacillus,Sulfobacillus,Thaumarchaeota), in agreement with thermodynamic calculations.
Zinc-binding metallophores protectPseudomonas aeruginosafrom calprotectin-mediated metal starvation
Authors: Ammendola Serena , Secli Valerio , Pacello Francesca , Mastropasqua Maria Chiara , Romão Mariana A , Gomes Cláudio M , Battistoni Andrea 
Published: July 2022

AbstractPseudomonas aeruginosa is known to exhibit considerable resistance to the antimicrobial activity of the metal-sequestering protein calprotectin (CP). In this study, we demonstrate that although CP induces zinc deficiency in P. aeruginosa, a strain unable to import zinc through the two most important metal acquisition systems, namely ZnuABC and ZrmABCD, maintains significant growth capacity in the presence of high concentrations of CP. Furthermore, we have shown that nicotianamine, a molecule structurally similar to the metallophore pseudopaline, can favor the acquisition of the metal even in the presence of CP. To gain insights into the mechanisms through which metallophores can promote zinc acquisition, we analyzed the effect of nicotianamine on the activity of the metallo-β-lactamase VIM-1. Our data suggest that metallophores released by bacteria in response to zinc deficiency can extract the protein-bound metal. The ability to interfere with the binding of metals to proteins, as well as favoring the acquisition of zinc, may contribute to increasing the resistance of P. aeruginosa to the antimicrobial action of CP.
Barriers to genetic manipulation of Enterococci: Current Approaches and Future Directions
Authors: Krause Alexandra L , Stinear Timothy P , Monk Ian R 
Published: July 2022

Abstract
Enterococcus faecalis and Enterococcus faecium are Gram-positive commensal gut bacteria that can also cause fatal infections. To study clinically relevant multi-drug resistant E. faecalis and E. faecium strains, methods are needed to overcome physical (thick cell wall) and enzymatic barriers that limit the transfer of foreign DNA and thus prevent facile genetic manipulation. Enzymatic barriers to DNA uptake identified in E. faecalis and E. faecium include type I, II and IV restriction modification systems and CRISPR-Cas. This review examines E. faecalis and E. faecium DNA defence systems and the methods with potential to overcome these barriers. DNA defence system bypass will allow the application of innovative genetic techniques to expedite molecular-level understanding of these important, but somewhat neglected, pathogens.
A Chromosome-Length Reference Genome for the Endangered Pacific Pocket Mouse Reveals Recent Inbreeding in a Historically Large Population
Authors: Wilder Aryn P , Dudchenko Olga , Curry Caitlin , Korody Marisa , Turbek Sheela P , Daly Mark , Misuraca Ann , Wang Gaojianyong , Khan Ruqayya , Weisz David , Fronczek Julie , Aiden Erez Lieberman , Houck Marlys L , Shier Debra M , Ryder Oliver A , Steiner Cynthia C 
Published: July 2022

Abstract
High-quality reference genomes are fundamental tools for understanding population history, and can provide estimates of genetic and demographic parameters relevant to the conservation of biodiversity. The federally endangered Pacific pocket mouse (PPM), which persists in three small, isolated populations in southern California, is a promising model for studying how demographic history shapes genetic diversity, and how diversity in turn may influence extinction risk. To facilitate these studies in PPM, we combined PacBio HiFi long reads with Omni-C and Hi-C data to generate a de novo genome assembly, and annotated the genome using RNAseq. The assembly comprised 28 chromosome-length scaffolds (N50 = 72.6 MB) and the complete mitochondrial genome, and included a long heterochromatic region on chromosome 18 not represented in the previously available short-read assembly. Heterozygosity was highly variable across the genome of the reference individual, with 18% of windows falling in runs of homozygosity (ROH) &gt;1 MB, and nearly 9% in tracts spanning &gt;5 MB. Yet outside of ROH, heterozygosity was relatively high (0.0027), and historical Ne estimates were large. These patterns of genetic variation suggest recent inbreeding in a formerly large population. Currently the most contiguous assembly for a heteromyid rodent, this reference genome provides insight into the past and recent demographic history of the population, and will be a critical tool for management and future studies of outbreeding depression, inbreeding depression, and genetic load.
Transmembrane helices 5 and 12 control transport dynamics, substrate affinity, and specificity in the elevator-type UapA transporter
Authors: Dimakis Dimitris , Pyrris Yiannis , Diallinas George 
Published: July 2022

Abstract
An increasing number of solute transporters have been shown to function with the so-called sliding-elevator mechanism. Despite structural and functional differences, all elevator-type transporters use a common mechanism of substrate translocation via reversible movements of a mobile core domain (the elevator) hosting the substrate binding site along a rigid scaffold domain stably anchored in the plasma membrane via homodimerization. One of the best-studied elevator transporters is the UapA uric acid-xanthine/H+ symporter of the filamentous fungus Aspergillus nidulans. Here, we present a genetic analysis for deciphering the role of transmembrane segments (TMS) 5 and 12 in UapA transport function. We show that specific residues in both TMS5 and TMS12 control, negatively or positively, the dynamics of transport, but also substrate binding affinity and specificity. More specifically, mutations in TMS5 can lead not only to increased rate of transport but also to an inactive transporter due to high-affinity substrate-trapping, whereas mutations in TMS12 lead to apparently uncontrolled sliding and broadened specificity, leading in specific cases to UapA-mediated purine toxicity. Our findings shed new light on how elevator transporters function and how this knowledge can be applied to genetically modify their transport characteristics.
Aging and Emotion Regulation Tactics Across the Historical Events of 2020
Authors: Wolfe Hannah E , Isaacowitz Derek M 
Published: July 2022

Abstract

Background and Objectives
Despite well-documented cognitive and physical declines with age, older adults tend to report higher emotional well-being than younger adults, even during the coronavirus disease 2019 (COVID-19) pandemic. To understand this paradox, as well as investigate the effects of specific historical contexts, the current study examined age differences in emotion regulation related to the events of 2020 in the United States. We predicted that, due to older adults’ theorized greater prioritization of hedonic goals and avoidance of arousal, older adults would report more positivity-upregulation and acceptance tactics than younger adults.

Research Design and Methods
Eighty-one younger adults (aged 18–25) and 85 older adults (age 55+) completed a retrospective survey on their emotion regulation tactic usage for 3 specific events: the COVID-19 pandemic outbreak, the killing of George Floyd, and the presidential election.

Results
Older adults tended to rely most on acceptance-focused tactics, while younger adults tended to rely on a more even variety of tactics. However, age differences in tactic preferences varied by event, possibly due to younger adults’ greater emotion regulation flexibility.

Discussion and Implications
Older adults’ higher emotional well-being may not be primarily a result of age differences in positivity-related emotion regulation tactics but more about differences in acceptance use.
A new 3-D finite-fault model of the Southwest Iceland bookshelf transform zone
Authors: Bayat Farnaz , Kowsari Milad , Halldorsson Benedikt 
Published: July 2022

SUMMARYThe largest earthquakes in Iceland occur in the South Iceland seismic zone (SISZ) and the Tjörnes fracture zone in the northeast. With the latter being primarily offshore, the seismic risk in Iceland is highest in the relatively densely populated SISZ. Past probabilistic seismic hazard assessment (PSHA) efforts in Iceland have, however, been based on statistical analyses of various historical earthquake catalogues and limited ground motion models (GMMs), all subject to varying types and degrees of uncertainties. Moreover, they relied on simplistic source descriptions and largely ignored that the unique ‘bookshelf’ strike-slip fault system of the SISZ extends along the plate margins towards the west and over the entire Reykjanes Peninsula Oblique Rift (RPOR) zone. Namely, the bookshelf fault system in Southwest Iceland is twice as long as previously thought and it dominates the strain release of transcurrent plate motion in Southwest Iceland, having potentially important implications for PSHA. In this study, therefore, we propose a new 3-D finite-fault model of the Southwest Iceland bookshelf transform zone. The model has been calibrated on the basis of first principles to the rate of transcurrent plate motions across the transform zone and constrained by the salient features of the fault system geometry as reported in the literature. We model the systematic spatial variability of the seismogenic potential along the zone by its provisional subdivision into six distinct zones. The fault system model allows both deterministic and random fault locations, with each realization completely specified in terms of the maximum expected magnitude of each fault, its maximum dimensions and its long-term slip rate. The variability of the model has been estimated through sensitivity analyses of its key parameters. The total seismic moment rates produced by the fault system model are completely consistent with those reported in the literature. The new model allows the derivation of simple but self-consistent zone-specific Gutenberg–Richter (GR) relationships, and the total long-term seismic activity predicted by the new 3-D fault system model effectively explains the historical earthquake catalogue of the SISZ–RPOR transform zone in Southwest Iceland. We are therefore confident that the model can serve as the foundation for future time-independent physics-based PSHA for Southwest Iceland. Moreover, the consistency and versatility of the model allows its application in conventional approaches to PSHA, which has the potential of bridging the gap between physics-based and conventional approaches to PSHA in Southwest Iceland. Such efforts will improve our understanding of the key elements that affect the hazard, thus improving the reliability of hazard estimates, with important practical implications for the optimized assessment of seismic risk.