bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Compact, Ultra-thin UWB Microstrip Antenna for Microwave Imaging Applications
Authors: Samadianfard Rasoul , Nourinia Javad , Ghobadi Changiz , Shokri Majid , samadianfard Roghayeh 
Published: October 2022

Abstract
This article deals with the study, design, fabrication, and measurement of a ultra-thin ultra-wideband (UWB) microstrip antenna. As the proposed antenna design is compact, ultra-thin, and optimized in size, it can be used for microwave imaging and wearable applications. Innovation has been used to design the UWB microstrip antenna, which has increased the efficiency and performance of the antenna. This innovation has considered the feed structure, geometry, modifications in the slots, and the use of an ultra-thin dielectric substrate with a thickness of 0.25 mm. The proposed technique is a unique tuning mechanism located under the feed line of the proposed UWB microstrip antenna. The antenna's compact and ultra-thin design is desirable for reducing the complexity of the physical array structure and achieving a degree of body fit. Parametric studies are performed to compare this performance after changes in patch and ground plane length. Finally, the simulated performance parameters were analyzed and compared with the measured results. Due to its small size, new shape, and impressive functions, the proposed antenna is well suited to multifunctional healthcare applications and sports performance monitoring.
Fluorescein diacetate hydrolytic activity as a sensitive tool to quantify nitrogen/sulfur gene content in urban river sediments in China
Authors: Zhang Chao , Li Meng , Sun Jingmei , Huang Jianjun , Chang Suyun 
Published: October 2022

Abstract
The relative abundance of functional genes used to quantify the abundance of functional genes in communities is controversial. Quantitative PCR (qPCR) technology offers a powerful tool for quantifying functional gene abundance. However, humic substances can inhibit qPCR in sediment/soil samples. Therefore, finding a convenient and effective quantitative analysis method for sediment/soil samples is necessary. The functional genes and physicochemical properties in sediments with different-level pollutions were analyzed in this study. Correlations between physicochemical properties and the relative abundance of functional genes were used to test whether relative abundance in gene prediction quantifies the abundance of functional genes. The abundance of functional genes could be corrected by multiplying the fluorescein diacetate (FDA) hydrolytic rates by the relative abundance of functional genes since the FDA assay has been widely used as a rapid and sensitive method for quantifying microbial activity in sediments. Redundancy analysis showed significant interrelations between the functional genes and the physicochemical properties of sediments. The relative abundance of functional genes is unreliable for quantifying the abundance of functional genes because of the weak correlation (R < 0.5, P < 0.05) between different pollutants and the relative abundance of functional genes. However, a significant positive correlation between concentrations of different pollutants and the activities of associated enzymes was obtained (R > 0.933, P < 0.05), which revealed that the abundance of functional genes could be reliably quantified by the relative abundance and FDA hydrolytic rate. This study proposed an alternative method besides qPCR to quantify the absolute abundance of functional genes, which overcomes the problem of humic interference in the quantitative analysis of sediment/soil samples.
The Prognostic Potential of Cuproptosis and Cuproptosis-Related Alteration of Clear Cell Renal Cell Carcinoma
Authors: Xing Zhaoyu , Cui Li , Feng Yuehua , Yang Yang , He Xiaozhou 
Published: October 2022

Abstract
Aim
To study whether the novel copper ionophores induced cell death would impact the prognosis of clear cell renal cell carcinoma (ccRCC) and subsequently influence the tumor microenvironment (TME).
Methods
The differential expression of 10 cuproptosis and 40 TME-pathway-related genes were measured between 531 tumor samples and 71 adjacent kidney samples in The Cancer Genome Atlas (TCGA) database. The risk score model was constructed with LASSO-cox to predict the prognosis of ccRCC patients in the training cohort (331 patients). Afterward, the risk model was reinforced in the validation cohort and the entire cohort. To study the biological function of the hub genes in-depth, nomogram and function enrichment were employed. Subsequently, the ESTIMATE analysis, TMEscore analysis and CIBERSORT algorithm were employed to To further observe the impact of these hub-genes on the TME.
Results
Differentially expressed genes (DEGs) were identified between tumor and normal samples. Twenty-one DEGs were used for the construction of LASSO-cox regression and a risk model with 10 hub genes were screened out to predict the prognosis of ccRCC patients. Patients from the High-Score group had a worse overall survival (OS). Besides, though many immunosuppressive cytokines encoding genes contributed to the High-Score group, this group showed a better immune response when compared to the Low-Score group. In addition, the High-Score group exhibited a significantly higher proportion of several anti-tumor lymphocytes and a lower proportion of immunosuppressive M2-like macrophages. However, the T follicular helper cells and regulatory T cells also showed higher proportion in the High-Score group.
Conclusion
The risk score model showed promising and predictive accuracy for the prognosis of ccRCC patients. Besides that, the cuproptosis based therapy is worth further studying regarding the ccRCC tumor and TME.
A unified approach to access β-branched organoboron through coupling of boron-stabilized organozinc with alkyl halides
Authors: Guo Panchi , Hao Jin , Han Jinhui , Xu Liang , Li Pengfei , Zhan Miao 
Published: October 2022

AbstractA general and efficient strategy for the preparation ofβ-branched organoboron compounds through Ni-catalyzed coupling of BpinCH2ZnI with alkyl halides had been developed. In comparison with known methods such as Matteson homologation and the functionalization of 1,1-bisborylmethane, this reaction is more step-economical and has broader functional-group compatibility. Primary, secondary and the challenging tertiary alkyl halides are appropriate substrates to accomplish this transformation. The synthetic practicability of the preparedβ-quaternary organoboronates was demonstrated by their conversion to other useful compounds.
Frozen section in amniotic infection, an underutilized diagnostic tool?
Authors: Ladino Karen , Caicedo-Marmolejo Beatriz E , Vargas Yaris Anzully , Jaramillo Luis F , Olaya-C Mercedes 
Published: October 2022

Abstract
Introduction: Chorioamnionitis and funisitis produce high impact maternal, fetal and neonatal repercussions; intra-amniotic infections are present in 40-70% of preterm deliveries; additionally, intrauterine infections trigger systemic inflammatory conditions in 50% of exposed fetuses. On the contrary, maternal symptoms register low sensitivity for diagnosis of intrauterine infections. Herein, we discuss the need for a new diagnostic tool that will allow for early capture of subclinical cases.
Methods: Between 2020 and 2022, we included 30 patients born with (Group 1:15) or without (Group 2:15) suspected intra-amniotic infection . We compared frozen sections to final paraffin sections, we correlated conventional process to neonate clinical outcome, taking into account changes in treatment based on early diagnosis of the infection.
Results: Group 1 presented the largest number of cases for preterm delivery, low birth weight, complications and total hospital stay. Furthermore, in Group 1, we found 33.3% early sepsis diagnosis, as opposed to 6.67% in Group 2; following positive pathology report, both duration of antibiotic management and length of clinical observation: it was extended over a greater length of time than expected as standard.
Discussion: Research has validated the premise on which we based our study, a clear relation between chorioamnionitis and fetal death and preterm delivery, the outcome of which also results in an increase in comorbidities. Early diagnosis of infection, especially of funisitis, is clinically useful for subsequent intervention.
Conclusions: The use of frozen biopsy in ascending subclinical infection can aid in reaching early chorioamniontis/funisitis diagnosis, thereby providing guidance in medical interventions. This technique has proven its performance in predicting definitive infection outcomes. However, there is still a need for further studies on how to profile patients with subclinical infections who would benefit from frozen biopsy analysis.
Chitosan-modified magnetic carbon nanomaterials with high efficiency, controlled motility and reusability - for removal of chromium ions from real wastewater
Authors: Li zhishuncheng , Yang Can , Qu GuangFei , Cui Qingyuan , Yang Yixin , Ren Yuanchuan , Yang Yuyi , Wang Fang 
Published: October 2022

Abstract
Hexavalent chromium Cr(Ⅵ) is one of the most hazardous oxygen-containing anions to human health and the environment. Adsorption is considered to be an effective method for the removal of Cr(Ⅵ) from aqueous solutions. Based on an environmental perspective, we used renewable biomass cellulose as carbon source and chitosan as functional material to synthesize chitosan coated magnetic carbon（ MC@CS ）Materials. The synthesized chitosan magnetic carbons are uniform in diameter (~20 nm) and contain a large number of abundant hydroxyl and amino functional groups on the surface, meanwhile owning excellent magnetic separation properties. The MC@CS exhibited high adsorption capacity (83.40 mg/g) at pH 3 and excellent cycling regeneration ability when applied to Cr(VI) removal in water, removal rate of Cr(VI) (10mg/L) was still over 70% after 10 cycles. FT-IR and XPS spectra shows that electrostatic interaction and reduction with Cr (VI) are the main mechanisms of Cr (VI) removal by MC@CS nanomaterial. This work provide an environment-friendly adsorption material that could be reused for the removal of Cr(VI) in multiple cycles.
Integrating knowledge from Case Report: a medical-ontology based multimodal information system with structured summary
Authors: Bai Tian , Huang Lan , Guo Shuyu , Liu Yichen , Wu Minfei , Gu Guishan , Luo Xiao 
Published: October 2022

Abstract
Published Case Report literature, as an important medical information carrier, records invaluable new discoveries of rare diseases, diagnoses and novel therapies as well as unique clinical experiences. Millions of Case Reports following formatted content have been collected in PubMed sharing medical knowledge across worldwide healthcare community. However, the ability of searching and viewing a vast number of Case Reports, and grasping most relevant and essential content in a time-constrained environment has been severely limited with most existing keyword-based retrieval tools due to their inherent limitation in handling the scale, unstructuredness and heterogeneity of Case Reports. To alleviate such limitations, we have developed CRFinder, a comprehensive multimodal information system with powerful interfaces for searching, visualizing and analyzing Case Report. In this paper, we will discuss the extraction pipeline of clinical knowledge in Case Report and core functional components of proposed system. The efficacy of our pipeline and system is demonstrated using 52949 open access case reports published from 2000 to 2021 in PubMed Central, from which CRFinder builds structured case summary by automatically extracting clinical and biological entities, medical images of different modalities. All the different types of essential information can be conveniently and effectively searched, displayed and analyzed in a well-structured and correlated manner. The access of CRFinder system is now available online (http://49.235.104.183:8080/).
Isolation, purification, and identification of antifungal protein produced by Bacillus subtilis SL-44 and anti-fungal resistance in apple
Authors: Chen Wumei , Wu Zhansheng , He Yanhui 
Published: October 2022

Abstract
Apple anthracnose is a fruit fungal disease that is currently recognized as one of the most serious threats to apples worldwide. In this study, antifungal protein from B. subtilis SL-44 was isolated, purified, identified, and applied for apple anthracnose control. The antagonistic experiment showed that SL-44 had an excellent broad spectrum against plant pathogenic fungi. Initial pH, inoculum volume, and rotation speed were evaluated by single-factor experiments and Box-Behnken Design and which were optimized to be 7, 2%, and 180 rpm, respectively. Under these conditions, the yield of antifungal protein increased by 45.83% compared to that before optimization. The crude protein from SL-44 was isolated and purified by (NH4)2SO4 precipitation, DEAE-Sepharose fast flow column, and Sephadex G-100 column. TEM revealed that antifungal protein could severely disrupt the mycelial of C. gloeosporioides, resulting in the loss of its intracellular contents. LC-MS analyzed that antifungal protein was likely to be an uncharacterized protein YefB with a molecular weight of 35 kDa. Additionally, antifungal protein significantly alleviated the size of the lesion to more than 70% in the apple infection protection test. In conclusion, B. subtilis SL-44 and its main antimicrobial components have remarkable potential in the development of fungicides for biological control of apple anthracnose.
Bile Acids Are Substrates for Amine N-Acyl Transferase Activity by Bile Salt Hydrolase
Authors: Patterson Andrew , Rimal Bipin , Collins Stephanie , Granda Megan , Koo Imhoi , Solanka Sumeet , Hoque Nushrat , Gentry Emily , Yan Tingting , Bisanz Jordan , Krausz Kristopher , Desai Dhimant , Amin Shantu , Rocha Edson , Coleman James , Shah Yatrik , Gonzalez Frank , Heuvel John Vanden , Dorrestein Pieter , Weinert Emily 
Published: October 2022

Abstract
Bacteria in the gastrointestinal tract (GI) produce a variety of amino acid bile acid amidates that impact host-mediated metabolic processes; however, the bacterial gene(s) responsible for their production remain unknown. Herein, we report that bile salt hydrolase (BSH) possesses dual functions, including a new role in bile acid metabolism by functioning as an amine N-acyl transferase that conjugates amines to form bacterial bile acid amidates (BBAAs). To characterize this new amine N-acyl transferase role for BSH, we used pharmacological inhibition of BSH, heterologous expression of bsh in Escherichia coli, and generated a bsh knockout and knockin in Bacteroides fragilis to demonstrate that BSH is necessary and sufficient for BBAA production. Lastly, we report that BBAAs activate host ligand-activated transcription factors including the farnesoid X receptor, pregnane X receptor, constitutive androstane receptor, and the aryl hydrocarbon receptor. These new findings expand our understanding and appreciation for the important roles that bacteria play in shaping the bile acid metabolic network.
Bile salt hydrolase/aminoacyltransferase shapes the microbiome
Authors: Guzior Douglas , Okros Maxwell , Hernandez Christian Martin , Shivel Madison , Armwald Bruin , Hausinger Robert , Quinn Robert 
Published: October 2022

Abstract
Bile acids (BAs) are steroid detergents in bile that contribute to the absorption of fats and fat-soluble vitamins while shaping the gut microbiome due to their antimicrobial properties. Recently, a novel mechanism of BA metabolism by the gut microbiota was identified involving conjugation with various amino acids. Here, we show this unique biochemistry is mediated by bile salt hydrolase acting as an aminoacyltransferase or transpeptidase (BSH/T). Clostridium perfringens BSH/T rapidly performed acyl-transfer when provided various amino acids and taurocholate, glycocholate or cholate, with an optimum at approximately pH 5.3. Amino acids selected for incorporation by CpBSH/T were pH-dependent and broad in range, though proline and aspartic acid conjugates were not observed. BA conjugation was widespread among gut bacteria but showed unique amino acid preferences depending on the organism. Members of the family Lachnospiraceae had closely related BSH/T amino acid sequences and exhibited similar amino acid conjugation profiles, while horizontal gene transfer of bsh in some species increased conjugation diversity. Tertiary structure mapping of BSH/T from different gut bacteria implicates active site structure in amino acid selectivity. The products of BSH/T aminoacyltransferase activity were potent antimicrobials but this depended on the amino acid conjugated, with phenylalanine and leucine-conjugates acting as the best inhibitors of other gut commensals and known pathogens. This inhibitory property translated in vivo, as oral gavage of mice with different microbially conjugated bile acids altered the cecal and fecal microbiomes. This study shows that transpeptidase activity of BSH/T is a widespread and common property of the enzyme that greatly diversifies the bile acid pool, with the potential to shape the human microbiome.
Factors associated with subclinical inflammation of wrist joints in rheumatoid arthritis patients with low or no disease activity- A RA ultrasound registry study
Authors: Wang Yu-Wei , Chen Jia-Feng , Ko Chi-Hua , Cheng Tien-Tsai , Chiu Wen-Chan , Yu Shan-Fu , Hsu Chung-Yuan , Chen Ying-Chou 
Published: October 2022

Abstract
Background
Evaluate factors to predict subclinical inflammation of wrist joints in patients with RA who are in clinical remission or low disease activity
Methods
Gray scale and power Doppler ultrasound were performed on the dorsal radio-lunate of both wrists. The presence of synovitis, comorbidities, and use of disease modifying anti-rheumatic drugs were recorded. A Multivariable logistical regression model was used to identify factors associated with subclinical inflammation.
Results
There were 1248 patients (1010 females, 238 males; mean age: 60.0 ± 10.5 years ). 57.4% of patients in complete remission and low disease activity had sonographic inflammation. Multivariable logistic regression analysis indicated that male sex, smoking, and liver disease are positively associated with inflammation and that age, alcohol consumption, hypertension, gastrointestinal disease, and use of methotrexate, glucocorticoid, or a biological therapy are negatively associated with inflammation. Use of biological agents decreased the risk of inflammation by 40.9%.
Conclusions
There was evidence of subclinical inflammation in most patients who were in low or no disease activity, those with biological therapy had lower risk of subclinical inflammation.
WITHDRAWN: Molecular diagnosis of autosomal dominant and autosomal recessive congenital cataract families from North India
Authors: Goyal Shiwali , Singh Ravijit , Singh Jai Rup , Vanita Vanita 
Published: October 2022

Abstract
Background
To investigate the underlying genetic defects in two autosomal dominant (ADCC) and an autosomal recessive (ARCC) congenital cataract families from North India.
Methods
Detailed family history was collected, and pedigrees drawn followed by slit-lamp examination and lens photography. Mutation screening performed using Sanger sequencing in the known candidate genes for crystallins, connexins, and membrane proteins. Various bioinformatics tools were used to assess the pathogenicity of the identified variants.
Results
In two ADCC families with posterior lenticonus cataract, a novel change (c.263C > T; p.P88L) in GJA3 (CC-281 family) and a previously reported substitution (c.388C > T; p.R130C) in LIM2 in second ADCC family (CC-3015) was observed. In an ARCC family (CC-3005) having bilateral central pulverulent cataract, a novel frameshift deletion c.764delT; p.L255R46fs) in GJA3 was detected. The observed variants segregated completely with the phenotypes in the affected members and were neither present in unaffected family members nor in the ethnically matched 150 controls, hence excluding these as polymorphisms.
Conclusions
Present study identified two novel mutations i.e., c.263C > T (p.P88L) and c.764delT (p.L255R46fs) in GJA3 in an ADCC and an ARCC family having posterior lenticonus and central pulverulent cataract, respectively. In second ADCC family with posterior lenticonus cataract, a previously reported mutation c.388C > T (p.R130C) in LIM2 was observed. This indicates R130 a mutation hotspot as previously two ADCC families one each of European and Chinese descent also harbored the same substitution, however, with different phenotypes i.e., pulverulent and nuclear cataracts. Findings in the present study thus expand the mutation spectrum and phenotypic heterogeneity linked with GJA3 and LIM2.
Do Operational Facilitating Conditions and Subjective Norms Improve the Intention to use Social Distancing Nudge during a Pandemic: A Case of SARS-CoV-2(COVID-19) Outbreak in India
Authors: Kumar Anup 
Published: October 2022

Abstract
The COVID-19 outbreak reached a critical stage where it became imperative for public health systems to act decisively and design potential behavioral operational strategies that aim at containment of the pandemic. Isolation through social distancing plays a key role in achieving this objective. This research study is conducted to examine the factors affecting the intention of individuals towards social distancing in India. Correlation survey study is conducted on the samples of Pan Indian (N = 499) residents. Online questionnaire was floated consisting of Health Belief Model, and Theory of Planned Behavior Model, with respect to social distancing behavior at an initial occasion. Finally, Structural equation modeling is used to test the hypotheses. The results show that Perceived susceptibility, Facilitating Conditions and Subjective Norms are the major predictors of Attitude towards social distancing with the effect size of 0.277, 0.132, and 0.551 respectively. The result also confirms that the Attitude towards social distancing, perceived Usefulness of social distancing, and Subjective Norms significantly predicted the Intention to use the social distancing with the effect size of 0.355, 0.197, and 0.385 respectively. The non-significant association of Perceived Susceptibility(PS) with Social Distancing Intention (IN) (H1b) is rendering the fact that Attitude (AT) mediates the relationship between PS and IN similarly, the non-significant association of Facilitating Conditions (FC) with IN (H5) is rendering the fact that AT mediates the relationship between FC and IN. Results of the study is helpful to the policy makers to handle operations management of nudges like social distancing. The research is one of its kind that explores the behavioral aspects of handling social nudges.