bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of peracetic acid by spray or immersion in chicken carcasses to reduce cross‐contamination in the slaughter process
Authors: Laranja Daniela Comparsi , Cacciatore Fabíola Ayres , Malheiros Patrícia da Silva , Tondo Eduardo Cesar 
Published: October 2022

AbstractThe poultry industry uses several strategies during poultry processing to increase the safety and shelf life of fresh products. One possible intervention is the application of peracetic acid (PAA) on carcasses by spraying or immersion. This study aimed to evaluate the antimicrobial action of PAA and its capacity to prevent cross‐contamination when applied on chicken carcasses. First, the method of PAA application that achieved the greatest reduction against a Salmonella cocktail artificially inoculated into chicken carcasses and against naturally present microorganisms was determined. We used the maximum concentration allowed in the United States (0.02% PAA) determining that immersion method was more effective than spray. Next, the reduction of the same microorganisms was evaluated by immersing carcasses in 0.07% PAA. This concentration was defined in a previous study as the most effective post‐chiller without causing changes in quality. Finally, the reduction of cross‐contamination between 4 carcasses consecutively immersed in 0.07% PAA was investigated. The immersion in 0.07% PAA was able to reduce ~2.0 log CFU/ml of aerobic bacteria and Salmonella, and ~1.5 log CFU/ml of Enterobacteriaceae and Escherichia coli. The results of the cross‐contamination test suggest that rinse water containing 0.07% PAA could reduce contamination between carcasses and in the post‐chiller tank water. Therefore, 0.07% PAA showed potential to contribute to the safety and increased shelf life of chicken carcasses.
A prolific and robust whole‐genome genotyping method using PCR amplification via primer‐template mismatched annealing
Authors: Zhao Sheng , Zhang Cuicui , Wang Liqun , Luo Minxuan , Zhang Peng , Wang Yue , Malik Waqar Afzal , Wang Yue , Chen Peng , Qiu Xianjin , Wang Chongrong , Lu Hong , Xiang Yong , Liu Yuwen , Ruan Jue , Qian Qian , Zhi Haijian , Chang Yuxiao 
Published: October 2022

ABSTRACTWhole‐genome genotyping methods are important for breeding. However, it has been challenging to develop a robust method for simultaneous foreground and background genotyping that can easily be adapted to different genes and species. In our study, we accidently discovered that in adapter ligation‐mediated PCR, the amplification by primer‐template mismatched annealing (PTMA) along the genome could generate thousands of stable PCR products. Based on this observation, we consequently developed a novel method for simultaneous foreground and background integrated genotyping by sequencing (FBI‐seq) using one specific primer, in which foreground genotyping is performed by primer‐template perfect annealing (PTPA), while background genotyping employs PTMA. Unlike DNA arrays, multiple PCR, or genome target enrichments, FBI‐seq requires little preliminary work for primer design and synthesis, and it is easily adaptable to different foreground genes and species. FBI‐seq therefore provides a prolific, robust, and accurate method for simultaneous foreground and background genotyping to facilitate breeding in the post‐genomics era.
Midwifery Care of Pregnant Individuals Experiencing Opioid use Disorder: Changing Regulations, Complexities, and Call to Action
Authors: Bukowski Hannah Bartee , Combellick Joan L 
Published: October 2022

AbstractOpioid use disorder (OUD), overdose, and death have exploded in the United States in the past 2 decades. The number of pregnant and birthing people reporting opioid use and misuse is also rising. Co‐occurring mental illness, multisubstance use, and associated medical comorbidities often complicate care for pregnant individuals with OUD. Neonates who are exposed to opioids in utero are at risk for neonatal opioid withdrawal syndrome and other short‐ and long‐term sequelae. Recent changes to the Department of Health and Human Services Practice Guidelines for the Administration of Buprenorphine for Treating Opioid Use Disorder have now provided a pathway for midwives to prescribe buprenorphine for opioid use disorder (OUD) for up to 30 individuals at one time without further training or certification of ancillary services. Midwives have a key role to play in expanding the availability and quality of interprofessional care provided to individuals with OUD. The Substance Abuse and Mental Health Services Administration and American Society of Addiction Medicine, along with other professional organizations, provide toolkits and guidelines for the provision of MOUD for pregnant people. Midwives who care for individuals with OUD should be familiar with the unique needs of this population and resources to guide their care. This case study highlights midwives’ essential role in treating OUD and co‐occurring mental disorders.
Continued P2X7 activation leads to mitochondrial fission and compromising microglial phagocytosis after subarachnoid haemorrhage
Authors: Tao Tao , Chen Xiangxin , Zhou Yan , Zheng Qiang , Gao Sen , Wang Jinwei , Ding Pengfei , Li Xiaojian , Peng Zheng , Lu Yue , Gao Yongyue , Zhuang Zong , Hang Chun‐hua , Li Wei 
Published: October 2022

AbstractSubarachnoid haemorrhage (SAH) has a high rate of disability and mortality. Extremely damaging molecules, including adenosine triphosphate (ATP), are released from extravasated red blood cells and nerve cells, which activate microglia and induce sterile tissue injury and organ dysfunction. P2X purinoceptor 7 (P2X7) is one of the most important purine receptors on the microglial surface and is involved in the proinflammatory activation of microglia. While P2X7 can also affect microglial phagocytosis, the mechanism is not clear. Here, we demonstrated that microglial phagocytosis is progressively impaired under continued BzATP exposure and P2X7 activation. Furthermore, we found that P2X7 activation leads to increased intracellular Ca2+ levels and activates Calcineurin, which dephosphorylates dynamin‐related protein 1 (DRP1) S637. The dephosphorylation of DRP1 at S637 leads to increased mitochondrial fission and decreased mitochondrial function, which may be responsible for the decreased microglial phagocytosis. Finally, we pharmacologically inhibited P2X7 activation in mice, which resulted in rescue of mitochondrial function and decreased microglial proliferation, but improved phagocytosis after SAH. Our study confirmed that P2X7 activation after SAH leads to the impairment of microglial phagocytosis through mitochondrial fission and verified that P2X7 inhibition restores microglial phagocytosis both in vitro and in vivo.image
The effect of sacral massage on meconium‐stained amniotic fluid and the duration of fetal descent in labor: A randomized controlled trial
Authors: Ulubasoglu Hasan , Bedir Fındık Rahime , Keskin Hüseyin Levent , Özcan Damla , Tekin Ozlem Moraloglu , Karakaya Jale 
Published: October 2022

AbstractObjectiveThe aim of this study was to investigate the effect of sacral massage on the presence of meconium‐stained amniotic fluid and the duration of fetal descent during labor in pregnant women.MethodsA total of 220 nulliparous women with singleton low‐risk pregnancies in the vertex position at term were recruited. Eligible women were randomly assigned to either massage group or a control group. The massages were performed with friction and tapotement protocol. The massage was applied for 10 min each time by a midwife who is also an expert in the field, at the onset of contractions during the intrapartum period, when cervical dilatation reached 10 cm. The Wong–Baker faces pain rating scale was evaluated.ResultsNo significant difference was found between the length of the first stage of labor and the total length of delivery (p = 0.097 and 0.434), respectively. There was a significant difference between the two groups in terms of perineal injuries. Perineal injury was lower in the massaged group (p = 0.005). There was a low percentage of meconium‐stained amniotic fluid in the massaged group. The difference between the groups was statistically significant. The duration of fetal descent was shorter in the massaged group (p < 0.001). A significant difference was found in Wong–Baker FACES pain rating scale scores. Lower scores were detected in the massaged group (p < 0.001).ConclusionSacral massage has beneficial effects on mothers and babies in obstetric practice. In addition, applying massage during labor plays a significant role in reducing the presence of meconium‐stained amniotic fluid and the duration of fetal descent.
Validation of eligibility criteria for the Japan Obstetric Compensation System for Cerebral Palsy in preterm infants: A case–control cohort study
Authors: Kusuda Satoshi , Nakanishi Hidehiko , Suzuki Hideaki ,   the Neonatal Research Network of Japan
Published: October 2022

AbstractAimTo verify validity of the criteria used for the Japan Obstetric Compensation System for Cerebral Palsy (JOCS‐CP) in preterm infants, the association between the criteria and the development of CP was studied using a neonatal database. Our hypothesis was that the criteria would not be sufficient due to the recent advances made in perinatal care.MethodsPreterm infants born between 2003 and 2019 and registered in the Neonatal Research Network of Japan database with a birth weight of 1500 g or less or a gestational age of less than 32 weeks were analyzed. The database included the clinical information of registered infants during their stay in NICUs and outcomes at 3 years of age.ResultsThe database included 73 615 infants. After excluding those with an unknown outcome at discharge, 73 464 infants were analyzed for short‐term outcomes, including mortality and morbidities. The incidence of CP at 3 years of age was analyzed in 36 151 infants. Furthermore, 16 467 infants born between 28 and 31 weeks of gestation were examined in terms of the validity of the current eligibility criteria. The mortality and incidences of severe intraventricular hemorrhage and periventricular leukomalacia significantly decreased during the study period (Cochrane–Armitage test, p < 0.01). Furthermore, the eligibility criteria were not sufficiently nor strongly associated with indicators for detecting perinatal hypoxia‐ischemia resulting in CP.ConclusionThe existing eligibility criteria of the JOCS‐CP used for preterm infants born between 28 and 31 weeks were no longer suitable because of the advances in perinatal care in Japan.
Associations between myelin water imaging and measures of fall risk and functional mobility in multiple sclerosis
Authors: Edwards Erin M. , Stanley Jeffrey A. , Daugherty Ana M. , Lynn Jonathan , Borich Michael R. , Fritz Nora E. 
Published: October 2022

AbstractBackground and PurposeMyelin water fraction (MWF) deficits as measured by myelin water imaging (MWI) have been related to worse motor function in persons with multiple sclerosis (PwMS). However, it is unknown if measures from MWI metrics in motor areas relate to fall risk measures in PwMS. The objective of this study was to examine the relationship between MWI measures in motor areas to performance on clinical measures of fall risk and disability in PwMS.MethodsSixteen individuals with relapsing‐remitting MS participated (1 male, 15 female; age 47.1 years [12.3]; Expanded Disability Status Scale 4.0 [range 0‐6.5]) and completed measures of walking and fall risk (Timed 25 Foot Walk [T25FW] and Timed Up and Go). MWF and the geometric mean of the intra‐/extracellular water T2 (geomT2IEW) values reflecting myelin content and contribution of large‐diameter axons/density, respectively, were assessed in three motor‐related regions.ResultsThe geomT2IEW of the corticospinal tract (r = –.599; p = .018) and superior cerebellar peduncles (r = –.613; p = .015) demonstrated significant inverse relationships with T25FW, suggesting that decreased geomT2IEW was related to slower walking. Though not significant, MWF in the corticospinal tract and superior cerebellar peduncles also demonstrated fair relationships with the T25FW, suggesting that worse performance on the T25FW was associated with lower MWF values.ConclusionsMWI of key motor regions was associated with walking performance in PwMS. Further MWI studies are needed to identify relationships between pathology and clinical function in PwMS to guide targeted rehabilitation therapies aimed at preventing falls.
Natural variation of ZmLNG1 alters organ shapes in maize
Authors: Wang Qin , Fan Jiongjiong , Cong Jia , Chen Mengjiao , Qiu Jie , Liu Jie , Zhao Xiaoyan , Huang Ruipeng , Liu Hongjun , Huang Xuehui 
Published: October 2022

Summary
The development of a series of elite maize hybrids has greatly increased crop yield in the past decades. Parental lines of these hybrids usually come from different heterotic groups and contain many genetic differences. Identifications of important quantitative trait genes in the elite hybrids can extend our understanding of heterosis and also help to guide genetic improvement.
Here, we mapped a major quantitative trait locus using a linkage population from an elite maize hybrid Zhengdan958 and identified ZmLNG1 as the causative gene controlling multiple morphologic traits in maize. A 6‐kb deletion in one parental line of the hybrid leads to the fusion of ZmLNG1 with its nearby gene. The fusion event prevents the C‐terminal of ZmLNG1 from interacting with ZmTON1, which resulted in the change of plant architecture.
Further experiments demonstrated that ZmLNG1 could act as a mediator to connect ZmTON1 and ZmOFPs, which belong to another type of plant morphological regulatory proteins, thereby affecting the phosphorylation level of ZmOFPs.
These results demonstrate the importance of ZmLNG1 in forming the TON1‐TRM‐PP2A complex and provide a model for the regulation of plant organ morphology by TON1‐recruiting motifs (TRMs) and Ovate family proteins (OFPs).
UltraSound Axillary Vein Access (USAA): Learning curve and randomized comparison to traditional venous access for cardiac device implantation
Authors: Courtney Alex M. , Chandler Jonathan K. , Anderson John , Shrestha Amit , Noheria Amit , Pimentel Rhea , Dendi Raghuveer , Ramirez Rigoberto , Reddy Y. Madhu , Sheldon Seth H. 
Published: October 2022

AbstractBackgroundMany techniques exist for venous access (VA) during cardiac implantable electronic device (CIED) implantation.ObjectiveWe sought to evaluate the learning curve with ultrasound (US) guided axillary vein access (USAA).MethodsSingle‐center prospective randomized controlled trial of patients undergoing CIED implantation. Patients were randomized in a 2:1 fashion to USAA versus conventional VA techniques. The primary outcomes were the success rates, VA times and 30‐day complication rates.ResultsThe study included 100 patients (age 68 ± 14 years, BMI 27 ± 4 kg/m2). USAA was successful in 66/70 implants (94%). Initial attempts at conventional VA included 47% axillary (n = 14), 30% (n = 9) cephalic, and 23% (n = 7) subclavian. The median access time was longer for USAA than conventional access (8.3 IQR 4.2–15.3 min vs. 5.2 IQR 3.4–8.6 min, p = .009). Among the five inexperienced USAA implanters, there was a significant improvement in median access time from first to last tertile of USAA implants (17.0 IQR 7.0–21.0 min to 8.6 IQR 4.5–10.8 min, p = .038). The experienced USAA implanter had similar access times with USAA compared with conventional access (4.0 IQR 3.3–4.7 min vs. 5.2 IQR 3.4–8.6 min, p = .15). Venograms were less common with USAA than conventional access (2% vs. 33%, p < .0001). The 30‐day complication rate was similar with USAA (n = 4/70, 6%) versus conventional (n = 3/30, 10%, p = .44).ConclusionAlthough the success rate with USAA was high, there was a significant learning curve. Once experienced with the USAA technique, there is the potential for reduced complications without adding to the procedure duration.
The genome‐wide DNA methylation profiles among neurosurgery patients with and without post‐operative delirium
Authors: Yamanashi Takehiko , Crutchley Kaitlyn J. , Wahba Nadia E. , Nagao Takaaki , Marra Pedro S. , Akers Cade C. , Sullivan Eleanor J. , Iwata Masaaki , Howard Mathew A , Cho Hyunkeun R. , Kawasaki Hiroto , Hughes Christopher G. , Pandharipande Pratik P. , Hefti Marco M. , Shinozaki Gen 
Published: October 2022

AimsThere is no previous study demonstrating the differences of genome‐wide DNA methylation (DNAm) profiles between patients with and without postoperative delirium (POD). We aimed to discover epigenetic (DNAm) markers that are associated with POD in blood obtained from patients before and after neurosurgery.MethodsPre‐ and post‐surgical blood DNA samples from 37 patients, including 10 POD cases, were analyzed using the Illumina EPIC array genome‐wide platform. We examined DNAm differences in blood from patients with and without POD. Enrichment analysis with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes terms were also conducted.ResultsWhen POD cases were tested for DNAm change before and after surgery, enrichment analyses showed many relevant signals with statistical significance in immune response related‐pathways and inflammatory cytokine related‐pathways such as “cellular response to cytokine stimulus”, “regulation of immune system process”, “regulation of cell activation”, and “regulation of cytokine production”. Furthermore, after excluding the potential effect of common factors related to surgery and anesthesia between POD cases and non‐POD controls, the enrichment analyses showed significant signals such as “immune response” and “T cell activation”, which are same pathways previously identified from an independent non‐surgical inpatient cohort.ConclusionsOur first genome‐wide DNAm investigation of POD showed promising signals related to immune response, inflammatory response and other relevant signals considered to be associated with delirium pathophysiology. Our data supports the hypothesis that epigenetics play an important role in the pathophysiological mechanism of delirium and suggest the potential usefulness of an epigenetics‐based biomarker of POD.
Atopic dermatitis‐like graft‐versus‐host disease treated with dupilumab
Authors: Larijani Mary , Zarowin Diana , Wohlschlaeger Anne , Perman Marissa J. , Treat James R. 
Published: October 2022

AbstractThe mainstay of treatment for atopic dermatitis (AD)‐like graft‐versus‐host disease (GVHD) in both pediatric and adult patients includes oral corticosteroids with or without other systemic immunosuppressive therapies. To our knowledge, we report the first case series of dupilumab in the treatment of AD‐like GVHD in a pediatric cohort of four patients, where we observed clinical improvement of GVHD as well as a reduction in itch in 3/4 (75%) patients. Our findings suggest that dupilumab is not only effective in treating AD‐like GVHD, but also reduces systemic immunosuppression in the pediatric transplant population. The ability to reduce the length and amount of immunosuppression as well as improve quality of life suggest that dupilumab may serve as a safe and effective therapeutic option in our transplant population with GVHD.
To centralize or decentralize: Mergers under price and quality competition
Authors: Guo Xiaomeng , Kuang Yunjuan , Ng Chi To 
Published: October 2022

This paper examines firms' post‐merger integration (PMI) strategy under price and service‐quality competition when facing either deterministic or stochastic demand. Although horizontal mergers are prevalent in practice and have been analyzed extensively in the literature, little attention has been paid to the choice of PMI level. We develop a game‐theoretic model, in which multiple firms compete on price and service quality with either deterministic or stochastic demand, and two of them decide whether and how to merge. The post‐merger firm can enjoy higher cost efficiency due to cost synergy and needs to choose between centralized and decentralized mergers (i.e., PMI level). A centralized merger allows centralized decision‐making and inventory pooling for the two participant firms, while a decentralized merger allows decentralized decision‐making and inventory transshipment between the participant firms. We highlight some interesting findings. First, under either deterministic or stochastic demand, we find that a centralized merger is not always beneficial since its collusion effect may induce the nonparticipant firm to adopt more aggressive strategies, and thus a decentralized merger may be more profitable and preferred by the post‐merger firm as it allows competition between the participants and leads to a more balanced market. Second, in a decentralized merger, stronger fixed cost synergy may backfire and hurt each participant as it could intensify service‐quality competition between the two participants, and risk pooling (via inventory transshipment) under stochastic demand may even reduce the participant firms' profits as it may intensify both service‐quality and price competition. Third, a participant firm's service quality, price, and in‐stock probability may be either improved or reduced after merging, depending on the PMI level, cost synergy, demand sensitivity, and demand uncertainty.
Comparison of the malocclusion and orthodontic treatment needs of Down syndrome and non‐syndromic subjects by using the dental aesthetics index
Authors: Syed Mohamed Alizae Marny Fadzlin , Wei Tan Zhen , Sean Cheh Jing , Rosli Tanti Irawati 
Published: October 2022

AbstractObjectiveTo evaluate the prevalence and significance of the malocclusion traits and orthodontic treatment needs of Down syndrome (DS) subjects with matched non‐syndromic (NS) controls.MethodologyThis cross‐sectional study involved 222 subjects (50% DS, 50% NS) who were matched by gender and age. The casts were digitized into three‐dimensional images. These images were used alongside the ten occlusal characteristics of the Dental Aesthetic Index (DAI) to determine malocclusion severity and the need for orthodontic treatment.ResultsThere were 58 (52.3%) females and 53 (47.7%) males with a mean age of 18.4 ± 8.4 years in both groups. The most common and significant (p < .05) malocclusion traits among the DS subjects were missing teeth (56.2%), incisal spacing segments (73.0%), anterior maxillary irregularity (95.5%), mandibular overjet (36.0%), and full cusp deviation of the molar relationship (61.3%). On the other hand, increased maxillary overjet (69.3%) is a more significant (p < .05) trait amongst NS subjects. There were 85 (76.6%) DS subjects who presented severe malocclusion, which implies a highly orthodontic treatment need (DAI > 30). The DS showed some impact of malocclusion traits components of the DAI.ConclusionThe occurrence of malocclusion was higher in DS, implying a higher need for orthodontic treatments than for NS subjects.