bluuebunny/crossref_metadata_2025_split huggingface dataset:
CVD risk from prolonged occupational sitting attenuated by additional physical activity – Evidence from a prospective cohort of 481,688 Asian adults
Authors: Gao Wayne , Sanna Mattia , Chen Yea-Hung , Tsai Min-Kuang , Lu Po-Jung , Wen Chi-Pang 
Published: January 2022

Abstract
BackgroundFor the first time, the 2020 WHO guidelines on physical activity recommend reducing sedentary behaviors due to their health consequences. Less is known on the effect of prolonged occupational sitting, especially in the context of low physical activity engagement.This study aims at quantifying cardiovascular risk associated with prolonged occupational sitting and determining the additional amount of physical activity that may be needed to attenuate it.MethodsA cohort comprising 481,688 participants in a health surveillance program in Taiwan was followed between 1996 and 2017, collecting data on occupational sitting time, leisure-time physical activity (LTPA) habits, lifestyle, and metabolic parameters. The all-cause and expanded cardiovascular disease (CVD + diabetes mellitus + kidney disease) mortality associated with three occupational sitting volumes (mostly sitting, alternating sitting and non-sitting, mostly non-sitting) was analyzed applying multivariate Cox regression models to calculate the hazard ratios (HRs) for all participants and by subgroups, including five levels of LTPA. Deaths in the first two years of follow-up were excluded to avoid reverse causality.ResultsThe study recorded 26,257 deaths during a mean follow-up period of 12.85 years. Individuals mostly sitting at work had a higher mortality risk than those mostly non-sitting, both from all causes (HR: 1.16, 95% CI: 1.11-1.20) and from expanded CVD (HR:1.46, 95% CI:1.35-1.58), after adjusting for gender, age, education, smoking, drinking, and body mass index. Individuals alternating sitting and non-sitting at work did not experience increased risk for all-cause mortality, compared to individuals mostly non-sitting at work (HR: 1.01, 95% CI: 0.97-1.05), but did experience higher risk of deaths due to expanded CVD (HR: 1.13, 95% CI: 1.04-1.23). Individuals engaged in low (15-29 min/day) or no (<15 min/day) LTPA, who mostly sit at work, would need to increase their LTPA by 15 and 30 minutes respectively to reduce their risk of mortality to that of similarly inactive individuals who mostly do not sit at work.ConclusionsAs part of modern lifestyles, prolonged occupational sitting is considered normal and has not received due attention, even though its deleterious effect has been largely proved. Alternating sitting and non-sitting at work, as well as an extra 15 to 30 min/day of LTPA, can attenuate the harms of prolonged occupational sitting. Thus, emphasizing the associated harms and suggesting workplace system changes could help the society to de-normalize this common behavior, similarly to the process of de-normalizing smoking.
Identification of Leptospira in Patients With Renal Failure Using Serological, Molecular and Pathological Based Techniques, in Shiraz, Iran
Authors: Sayyadi Mostafa , Hosseinzadeh Saeid , Abdollahpour Gholamreza , Shekarforoush Seyed Shahram , Samiei Azadeh , Hosseinzadeh Masoud 
Published: January 2022

Abstract
Leptospirosis is a relatively rare bacterial infection that affects people and animals caused by pathogenic species of Leptospira. The present study was conducted using nested polymerase chain reaction (PCR), microscopic agglutination test (MAT) and hematological and biochemical tests on 200 blood samples of renal disorders patients in Shiraz, Iran. Also nested-PCR assay and Warthin-Starry (WS) silver staining method was performed on 30 nephrectomised kidney sample. The frequency of pathogenic species of Leptospira infection in patients with renal disorders was 20 % and this infection was significantly correlated with BUN, anemia, RDW, MCV, MCH and hemoglobin levels (P < 0.01). MAT analysis showed that serum samples had positive titers against L. Grippotyphosa (13 samples), L. Ballum (6 sample), L. Pomona (3 samples), L. Canicola (2 samples), L. Icterohaemorrhagiae (1 sample) and L. Hardjo (1 sample) serovars. Twenty-three percent of the kidney samples from the patients with pyelonephritis were infected with the pathogenic species of Leptospira. This study showed that pathogenic Leptospira serovars are present in this area and in patients with renal disorders more attention should be paid to this zoonotic disease.
Direct and Indirect Relationships Between Impaired Sleep, Obesity Risk Factors and Overweight and Obesity
Authors: Eid Shireen Walid , Brown Rhonda Francis , Birmingham Carl Laird , Maloney Shane k. 
Published: January 2022

Abstract
PurposeThe relationship between impaired sleep and overweight/obesity may be explained by sleep-disrupting behaviour that are practised by overweight people (e.g. night-eating, insufficient physical activity [PA], electronic device use) and stress/affective distress. Thus, we evaluated whether sleep parameters predicted overweight/obesity after taking into account the behaviour and affective state.MethodsOnline questionnaires asked about sleep quality, night-eating, PA, electronic device use and stress/affective distress at T1 (baseline) and T2 (3-months later). Height, weight and waist and hip circumference were measured. PA and sleep were assessed over 24-hours on two occasions using actigraphy in 161 participants at T1 and T2.ResultsAt T1, high body mass index (BMI)/waist-to-hip ratio (WHR) and obesity category were together related to more sleep disturbances (subjective) and longer awake time (objective), after controlling covariates (e.g. watching TV) and demographics (e.g. older age, male gender). At T2, high WHR was predicted by older age and male gender after controlling T1 WHR, demographics and covariates. Mediational analyses showed that sleep disturbances mediated nocturnal indigestion (NI) to BMI, poor subjective sleep quality mediated NI to WHR and high daytime dysfunction mediated NI to obesity category relationships.ConclusionMore time spent awake during the night (experienced as more sleep disturbances) was related to overweight/obesity indices even after taking into account other obesity risk factors (e.g. night-eating, insufficient PA, affect) and demographics. Mediational results suggest that NI parsimoniously explained the impaired sleep - overweight/obesity relationship.Level of Evidence: Level III, evidence obtained from well-designed cohort.
Essential Oil of Piper Purusanum C.DC (Piperaceae) and Its Main Sesquiterpenes: Biodefensives Against Malaria and Dengue Vectors, Without Lethal Effect on Non-target Aquatic Fauna
Authors: de Oliveira André C. , Simões Rejane C. , Lima Carlos A. P. , Silva Felipe M. A. da , Nunamura Sergio M. , Roque Rosemary A. , Tadei Wanderli P. , Nunomura Rita C. S. 
Published: January 2022

Abstract
The mosquitoes vectors of the genus Aedes and Anopheles present resistance to several commercial insecticides, which are also toxic to non-predators targets. On the other hand, essential oils are a promising source of insecticides. Thus, in this work, the essential oil from the leaves of Piper purusanum was characterized by gas chromatography based approaches and evaluated as biodefensive against malaria and dengue vectors. The main compounds of P. purusanum essential oil were β-caryophyllene (57.05%), α-humulene (14.50%) and germacrene D (8.20%). The essential oil inhibited egg hatching (7.6 ± 1.5 to 95.6 ± 4.5%) caused larval death (LC50 from 49.84 to 51.60 ppm) and inhibited the action of acetylcholinesterase (IC50 of 2.29 µg/mL), which can be related to the mechanisms of action. On the other hand, the biological activity of β-caryophyllene, α-humulene and germacrene D were higher than the essential oil. In addition, these sesquiterpenes and essential oil did not show a lethal effect on Toxorhynchites splendens, Anisops bouvieri, Gambusia affinis and Diplonychus indicus (LC50 from 2098.80 to 7707.13 ppm), although D. indicus is more sensible (SI/PSF from 48.56 to 252.02 ppm) to essential oil, representing a natural alternative against these relevant vectors.
A Fungal Modified Material With High Uranium (VI) Absorption Capacity And Strong Anti-Interference Ability
Authors: Tan Ni , Ye Qiaorong , Liu Yaqing , Yang Yincheng , Ding Zui , Liu Lijie , Wang Duoduo , Zeng Chensi 
Published: January 2022

Abstract
With polydioxyethylene ether as the bridge chain, a new fungal modified material with diamidoxime groups was prepared by a series of uncomplex synthesis reaction. The orthogonal experiment obtained its optimized adsorption conditions as follows: the initial pH value 6.5, the initial uranyl concentration 40 mg L-1, the contact time 130 min, and the solid-liquid ratio 25 mg L-1. The maximum adsorption capacity of target material was 446.20 mg g-1, and it was much greater than that of the similar monoamidoxime material (295.48 mg g−1). The linear Langmuir (R2 = 0.9856) isotherm models and the linear pseudo-second-order kinetic model (R2 = 0.9931) fit the experimental data of uranium (VI) adsorption better, indicating the adsorption mechanism should mainly be the monolayer adsorption and chemical process. In addition, the relevant experiments exhibited the prepared material was of the good reuse and the excellent anti-interference performance, which suggested the new acquisition should also have well-applied prospect in the future.
Geographical Variability of Bacterial Communities of Cryoconite Holes of Andean Glaciers
Authors: Pittino Francesca , Ambrosini Roberto , Seeger Michael , Azzoni Roberto Sergio , Diolaiuti Guglielmina , Gazitúa Pablo Alviz , Franzetti Andrea 
Published: January 2022

Abstract
Cryoconite holes, ponds full of melting water with a sediment on the bottom, are hotspots of biodiversity on glacier surface and host dynamic micro-ecosystems on these extreme environments. They have been extensively investigated in different areas of the world (e.g., Arctic, Antarctic, Alps, and Himalaya), but no study so far has described the bacterial communities of the glaciers in the Andes, the world longest mountain range. In this study, we start filling this gap of knowledge and describe the bacterial communities of Southern Andes in three small (< 2 km2) high elevation (< 4200 m a.s.l.) glaciers of Central Andes (Iver, East Iver and Morado glaciers) and two large (> 85 km2) glaciers in Patagonian Andes (Exploradores and Perito Moreno glaciers) whose ablation tongues reach low altitude (< 300 m a.s.l). Results show that the bacterial communities were generally similar to those observed in the cryoconite holes of other continents. Indeed, the most abundant orders were Burkholderiales, Cytophagales, Sphingobacteriales, Actinomycetales, Pseudomonadales, Rhodospiarillales, Rhizobiales, Sphingomonadales and Bacteroidales. However, the bacterial communities differed between glaciers and both water pH and O2 concentration influenced the bacterial community composition.
Meta-analysis of the Efficacy of Hemoperfusion Combined With Hemodialysis in Treating Acute Severe Organophosphorus Pesticide Poisoning
Authors: Zeng Siyao , Ma Lei , Hu Xiaodong , Wang Cheng , Gou Yi , Li Yi 
Published: January 2022

Abstract
BackgroundAcute severe organophosphorus pesticide poisoning (ASOPP) is a common severe emergency in developing countries. At present, the incidence of complications and mortality are still very high, and there are some difficulties in treatment. More and more studies have found that hemoperfusion combined with hemodialysis can improve the prognosis of patients based on conventional emergency treatment, but there is a lack of evidence-based medical evidence. Our meta-analysis is mainly to clarify the efficacy of hemoperfusion combined with hemodialysis on ASOPP.MethodsWe searched several databases. After formulating relevant inclusion and exclusion criteria, qualified studies were included and data were extracted. The outcome indicators were cure rate, efficacy, incidence of complications, time for cholinesterase level to return to normal, coma time, time of hospitalization and atropine dosage. Results were analyzed using risk ratios (RR), odds ratios (OR), weighted mean difference (WMD), standard mean difference (SMD), and 95% confidential interval (95% CI). The cochrane collaboration tool was used for the assessment of the risk of bias of every included studies. In terms of statistical methods, we used RevMan software (version 5.3) and Stata (version 14 and 16) for data analysis.ResultsWe included 35 RCTs, including 2650 participants. Compared with the emergency conventional treatment group, the use of hemoperfusion combined with hemodialysis to treat ASOPP patients based on conventional emergency treatment significantly increased the cure rate [RR=1.20, 95% CI (1.16, 1.25), p<0.00001], improved the efficacy [OR=2.76, 95% CI (2.07, 3.67), p=0.000], and reduced the incidence of complications [RR=0.26, 95% CI (0.20, 0.34), p<0.00001] and also reduced the patient's time for cholinesterase level to return to normal [WMD=-4.05, 95% CI (-4.40, -3.70), p<0.00001], coma time [SMD=-2.62, 95% CI (-3.08, -2.15), p<0.00001], time of hospitalization [WMD=-5.80, 95% CI (-6.57, -5.02), p<0.00001], and atropine dosage [WMD=-194.81, 95% CI (-223.55, -166.08), p<0.00001].ConclusionHemoperfusion combined with hemodialysis can apparently improve the prognosis of patients and has a good effect in the treatment of acute severe organophosphorus pesticide poisoning. In the future, more scientifically designed large-scale, high-quality, multi-center randomized controlled trials are needed to further illustrate the significance of this study.
Spatial assessment of tap water safety in China and associated health impacts
Authors: Liu Mengjie , Graham Nigel , Wang Wenyu , Zhao Renzun , Lu Yonglong , Elimelech Menachem , Yu Wenzheng 
Published: January 2022

Abstract
The quality of municipal water supplies is of fundamental importance to public health and national security. Here, we assess the tap water quality in 31 provinces across China and examine the effects of natural and anthropogenic conditions on water quality and associated health risks. Precipitation is a crucial factor influencing the organic matter content and ionic conductivity in tap water, especially for arid and semi-arid regions. Although the concentration of disinfection byproducts (DBPs) is closely related to the organic matter content, the occurrence of highly toxic DBPs was more significantly affected by anthropogenic factors such as economic development and pollution emission. We confirmed nanofiltration as an effective point-of-use treatment to reduce the adverse effects of DBPs in public water supplies. Since DBPs in tap water is a long-term global problem, our results highlight the potential health hazards of drinking water brought about by social development and conclude that countries and regions with rapid development might face high DBP toxicity.
Development and Operationalization of a Data Framework to Assess Quality of Integrated Diabetes Care in the Fragmented Data Landscape of Belgium
Authors: Buffel Veerle , Danhieux Katrien , Bos Philippe , Remmen Roy , Olmen Josefien Van , Wouters Edwin 
Published: January 2022

Abstract
Background. To assess the quality of integrated diabetes care, we should be able to follow the patient throughout the care path, monitor his/her care process and link them to his/her health outcomes, while simultaneously link this information to the primary care system and its performance on the structure and organization related quality indicators. However the development process of such a data framework is challenging, even in period of increasing and improving health data storage and management. This study aims to develop an integrated multi-level data framework for quality of diabetes care and to operationalize this framework in the fragmented Belgium health care and data landscape.Methods. Based on document reviews and iterative expert consultations, theoretical approaches and quality indicators were identified and assessed. After mapping and assessing the validity of existing health information systems and available data sources through expert consultations, the theoretical framework was translated in a data framework with measurable quality indicators. The construction of the data base included sampling procedures, data-collection, and several technical and privacy-related aspects of linking and accessing Belgian datasets.Results. To address three dimensions of quality of care, we integrated the chronic care model and cascade of care approach, addressing respectively the structure related quality indicators and the process and outcome related indicators. The corresponding data framework is based on self-collected data at the primary care practice level (using the Assessment of quality of integrated care tool), and linked health insurance data with lab data at the patient level. Conclusion. In this study, we have described the transition of a theoretical quality of care framework to a unique multilevel database, which allows assessing the quality of diabetes care, by considering the complete care continuum (process and outcomes) as well as organizational characteristics of primary care practices.
Sir2-domain associated short prokaryotic Argonautes provide defence against invading mobile genetic elements through NAD+ depletion
Authors: Zaremba Mindaugas , Dakineviciene Donata , Golovinas Edvardas , Stankunas Edvinas , Lopatina Anna , Sorek Rotem , Manakova Elena , Ruksenaite Audra , Silanskas Arunas , Ašmontas Simonas , Grigaitis Rokas , Timinskas Kęstutis , Venclovas Česlovas , Siksnys Virginijus 
Published: January 2022

Abstract
Argonaute (Ago) proteins are found in all three domains of life. The so-called long Agos are composed of four major domains (N, PAZ, MID, and PIWI) and contribute to RNA silencing in eukaryotes (eAgos) or defence against invading mobile genetic elements in prokaryotes (pAgos). Intriguingly, the majority (~60%) of prokaryotic Agos (pAgos) identified bioinformatically are shorter (comprised of only MID and PIWI domains) and are typically associated with Sir2, Mrr or TIR domain-containing proteins. The cellular function and mechanism of short pAgos remain enigmatic. Here, we show that short pAgos from Geobacter sulfurreducens, Caballeronia cordobensis and Paraburkholderia graminis, together with the NAD+-bound Sir2-proteins form a stable heterodimeric Sir2/Ago complex that recognizes invading plasmid or phage DNA through the pAgos subunit and activates Sir2 subunit triggering the endogenous NAD+ depletion and cell death thus preventing the propagation of invading DNA. This is the first demonstration that short Sir2-associated pAgos provide defence against phages and plasmids and underscores the diversity of mechanisms of prokaryotic Agos.
The Anti-Ischemic Effects of PIK3IP1 in Reducing the Likelihood of Cardiac Cell Death are Mediated Through its Interactions with the ETA-PI3Kγ-AKT Axis
Authors: Park Jei Hyoung , Nho Kyoung Jin , Lee Ji Young , Yoo Yung Joon , Park Woo Jin , Cho Chunghee , Kim Do Han 
Published: January 2022

Abstract
Oxidative stress, caused by the accumulation of reactive oxygen species (ROS) during acute myocardial infarction (AMI), is one of the main factors leading to myocardial cell damage and programmed cell death. Phosphatidylinositol-3-kinase -AKT (PI3K-AKT) signaling is essential for regulating cell proliferation, differentiation, and apoptosis. Phosphoinositide-3-kinase (PI3K)-interacting protein 1 (PIK3IP1) is an intrinsic inhibitor of PI3K in various tissues, but its functional role during AMI remains unknown. In this study, the anti-ischemic role of PIK3IP1 in an in vitro AMI setting was evaluated using H9c2 cells. The MTT assay demonstrated that cell viability decreased significantly with treatment of H2O2 (200 -500 µM). The TUNEL assay results revealed substantial cellular apoptosis following treatment with 200 µM H2O2. Under the same conditions, the expression levels of hypoxia-inducible factor (HIF-1α), endothelin-1 (ET-1), bcl-2-like protein 4 (BAX), and cleaved caspase–3, were elevated, whereas those of PIK3IP1 and Bcl-2 decreased significantly. PIK3IP1 overexpression inhibited H2O2-induced, and PI3K-mediated, apoptosis; however, PIK3IP1 knockdown reversed this effect, suggesting that PIK3IP1 functions as an anti-apoptotic molecule. To identify both the upstream and downstream molecules associated with PIK3IP1, ET-1 receptor type-specific antagonists (BQ-123 and BQ-788) and PI3K subtype-specific antagonists (LY294002 and IPI-549) were used to determine the participating isoforms. Co-immunoprecipitation was performed to identify the binding partners of PIK3IP1. Our results demonstrated that ROS-induced cardiac cell death may occur through the ETA-PI3Kγ-AKT axis, and that PIK3IP1 inhibits binding with both ETA and PI3Kγ. Taken together, these findings reveal that PIK3IP1 plays an anti-ischemic role by reducing the likelihood of programmed cell death via interacting with the ETA-PI3Kr-AKT axis.
Low Serum Iron Status Is Associated With Low Incidence of Coronary Artery Disease In Women
Authors: Guo Shuren , Mao Xiaohuan , Li Xiaohua , Ouyang Huan 
Published: January 2022

Abstract
Background: Disorders of iron metabolism has been implicated in cardiovascular disease. However, the association of serum ferritin and coronary artery disease (CAD) remains inconsistent. Here, we investigated the associations of serum iron metabolism with the incidence of CAD, the severity of coronary artery stenosis, metabolic biomarkers, and 1-year restenosis after coronary artery revascularization. Methods: A total of 643 CAD patients and 643 healthy controls were enrolled to assess the associations of serum iron status with the presence of CAD, the severity of CAD, and 1-year rehospitalization after revasculation. Serum iron metabolism and other metabolic markers were measured in all subjects. All statistical analyses were analyzed using SPSS22.0 software and STATA statistical package.Results: Serum level of iron metabolism markers, including serum iron, ferritin, unsaturated transferrin iron binding capacity (UIBC), Total iron binding capacity (TIBC) levels, in CAD groups was significantly higher than the control group (P<0.001). UIBC and TIBC were negatively correlated with ferritin in both sexes. Serum level of iron (OR=0.806, 95% CI (0.687-0.944), UIBC (OR=0.919, 95% CI (0.852-0.992), and TIBC (OR=0.864, 95% CI (0.787-0.95) were found to have a protective role for CAD in women (P<0.05, Table 3). The OR for ferritin was significant in the both sexes (OR=1.029, 95% CI (1.002-1.058) in men, OR=1.02, 95% CI (1.005-1.034) in women, P<0.05). Conclusion: Low Serum level of iron, UIBC, TIBC and ferritin levels were found to have a protective role for CAD in women, but not in men. Elevated serum ferritin is independently and positively associated with CAD in men and women.