bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychological attachment orientation and long-term post-traumatic stress symptoms among family members of intensive care unit patients
Authors: Zhang Qiang , Knies Andrea K. , Pach Jolanta , Kimbrough Tara , Martinez Aida , Juthani Prerak , Tu Stephanie , Monin Joan K. , Vranceanu Ana-Maria , Hwang David Y 
Published: April 2022

Abstract
Background: Rates of PTSD symptoms are high amongst families of ICU patients following hospital discharge. Adult attachment theory describes how people respond when separated from loved ones, those who have an “anxious” attachment orientation are preoccupied with being psychologically close with their partners. There is growing evidence that a person’s attachment orientation correlates with development of PTSD in multiple populations, however, this has not yet been investigated among ICU families. The purpose of this paper is to determine whether anxious psychological attachment orientation among family members of ICU patients predicts long-term PTSD symptoms among families following ICU discharge. Methods: We enrolled a consecutive sample of family members of patients admitted to a single academic neuroscience ICU from November 2017 to September 2020. Near time of ICU discharge, in addition to collecting demographics, we identified each participant’s attachment orientation via a standardized Relationship Questionnaire. Six months after discharge or death, each participant completed the Impact of Events Scale-Revised (IES-R) to measure PTSD symptoms, with a score of > 24 indicative of clinically significant symptoms. We conducted a univariate analysis and an adjusted multivariate analysis to determine if anxious attachment orientation predicts 6-month PTSD.Results: Among 162 total participants, 10/27 (37.0%) participants with an anxious attachment orientation reported 6-month PTSD symptoms, compared to 24/135 (17.8%) of non-anxious participants (RR 2.08, 95% CI 1.13-3.84, p=0.02, RD 19.2%). In a subsequent univariate analysis of participant and patient covariates, anxious attachment orientation, participant Hispanic ethnicity, prior experience as a care partner of a patient with a disability, and participation in 3 or more formal ICU family meetings were all associated with 6-month PTSD symptoms. In a multiple logistic regression, anxious attachment remained an independent predictor of 6-month PTSD symptoms (OR 3.64, 95% CI 1.35-9.77, p=0.01), as did Hispanic ethnicity and participation in 3 or more ICU family meetings. Conclusions: An anxious psychological attachment orientation is associated with double the risk of long-term PTSD symptoms among family members of ICU patients. Future interventions designed to decrease rates of adverse psychological outcomes among ICU families may be higher yield if attention is paid to this high-risk subgroup.
Nanocell COVID-19 vaccine elicits iNKT-licensed dendritic cells to produce high affinity antibodies neutralizing Variants of Concern
Authors: Gao Steven , Amaro-Mugridge Nancy , Madrid-Weiss Jocelyn , Petkovic Nikolina , Vanegas Natasha , Visvanathan Kumar , Ganju Vinod , Marx Gavin , Williams Bryan , MacDiarmid Jennifer , Brahmbhatt Himanshu 
Published: April 2022

Abstract
For COVID-19 vaccines, high-affinity antigen-specific antibody, CD8+ T cell and memory B cell responses are essential to maximize protection against Variants of Concern (VOC). We report results in vitro, in mice and human volunteers immunized with bacterially-derived, non-living nanocells (EDVTM) packaged with bacterial plasmid expressing spike protein of SARS-CoV-2 and IFNγ stimulating adjuvant α-galactosylceramide (EDV-COVID-αGC). EDV-COVID-αGC is shown to elicit iNKT-licensed dendritic cell activation/maturation, follicular helper T cell cognate help to B cells to undergo germinal center based somatic hypermutation and production of high affinity antibodies able to neutralize Alpha, Beta, Gamma, Delta, and Omicron VOC including a memory B cell response. Type I and Type II interferon stimulation and S-specific CD8+ T cells was also achieved. EDV-COVID-αGC are lyophilized, stored and transported at room temperature.
Interleukin 20 receptor subunit beta（IL20RB） predicts poor prognosis and regulates immune cell infiltration in clear cell renal cell carcinoma
Authors: Zhang Haoxun , Liu Yiewen , Wang Bowen , Wang Chunyang 
Published: April 2022

Abstract
Background and objectiveEmerging evidence has proven the robust role of tumor mutation burden (TMB) and immune cell infiltration (ICI) in cancer immunotherapy. However, the precise effect of TMB and ICI on clear cell renal cell carcinoma (ccRCC) remains elusive and merits further investigation. Therefore, we aimed to identify the TMB-related genes in predicting prognosis and to explore the potential mechanisms of the identified Interleukin 20 receptor subunit beta (IL20RB) in ICI in ccRCC.MethodThe relative information of patients with ccRCC was obtained from The Cancer Genome Atlas database (TCGA). Immune-related genes were downloaded from the Immunology Database and Analysis Portal database. Cox regression analysis was used to identify prognosis-related immune genes for ccRCC. The relationship of IL20RB expression levels with clinicopathological parameters was analyzed using the “limma” and “survival” packages. Gene Expression Omnibus (GEO) and International Cancer Genome Consortium (ICGC) databases were used as external validation. Quantitative Real-time PCR (qRT-PCR) and western blots were used to validate the expression levels of IL20RB in tumor cells. Cell counting kit-8 (CCK-8) assay was used to examine the effect of IL20RB on the viability of ccRCC cells. Gene set enrichment analysis (GSEA) was introduced for the analysis of IL20RB-related signaling pathways. Tumor Immune Estimation Resource (TIMER) and Tumor and Immune System Interaction Database (TISIDB) were utilized to determine the correlation of IL20RB expression levels with tumor-infiltrating immune cells (TIICs). ResultsIL20RB was significantly overexpressed in different ccRCC tissues and cells. High IL20RB expression in ccRCC patients was associated with short overall survival, high tumor grade, and advanced TNM stage. After knockdown of IL20RB with small interfering RNA (siRNA) technology, ccRCC cells’ proliferation was significantly attenuated. Moreover, overexpression of IL20RB could increase the infiltration level of several immune cells, especially T follicular helper cells (Tfh), and overexpressed Tfh cells were correlated with poor prognosis in ccRCC.ConclusionsIL20RB may function as an immune-associated therapeutic target for it determines cancer progression and regulates immune cell infiltration in ccRCC.
Live Birth Outcomes for PCOS Patients Under Depot Agonist Protocol or Antagonist Protocol - a retrospective Chinese cohort
Authors: Hao Guimin , Zhai Jiajia , Zhang Junyan , He Jingfang , Lv Minzhi , Zhang Bozheng , Du Yuanjie 
Published: April 2022

Abstract
Research question：When polycystic ovary syndrome (PCOS) patients undergo in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) and embryo transfer (ET), popular protocols include gonadotropin-releasing hormone agonist (GnRH-a) long protocol, GnRH antagonist protocol, and depot GnRH agonist protocol. There is no consensus regarding which protocol is the most optimal for PCOS patients, so we sought to evaluate depot GnRH agonist protocol versus GnRH antagonist protocol in terms of IVF outcome for PCOS patients in a single fertility center.Design：In this retrospective cohort, we included 533 PCOS patients who were undergoing their first IVF cycle. Among them, 470 were in the depot agonist group and 63 in the antagonist group. Fresh live birth rate (LBR) was the primary outcome. The secondary outcome was cumulative live birth rate (CLBR).Results：Women in the depot GnRH agonist group had a higher LBR (49.79%) than those in the antagonist group (34.92%), p=0.027. Multi-variable logistic regression showed that women in the depot GnRH agonist group had a higher LBR than those in the antagonist group (OR=1.83, p=0.032, 95% CI 1.05~3.18). After propensity score matching (PSM), the LBR in the depot GnRH agonist group was higher (50.32%) than that of the antagonist group (35.48%), p=0.033. Multi-variable logistic regression showed that women in the depot GnRH agonist group had a higher CLBR than those in the antagonist group (OR=1.77, p=0.038, 95% CI 1.03, 3.04). After PSM, CLBR in the depot GnRH agonist group was higher (60.00%) than that of the antagonist group (46.77%), p=0.054. Six patients (9.52%) in the antagonist group and 35 patients (7.45%) in the depot GnRH agonist group had ovarian hyper-stimulation syndrome (OHSS).Conclusions：In our experience, depot GnRH agonist can provide higher LBR and CLBR than the antagonist protocol can. Meanwhile, OHSS can be controlled at a satisfactory level for safe participation in both protocols.
Correction to: The Melnikov Method for detecting chaotic dynamics in a Planar Hybrid Piecewise-Smooth System with a Switching Manifold
Authors: Li Shuangbao , Gong Xiaojun , Zhang Wei , Hao Yuxin 
Published: April 2022

Abstract
Correction to: Nonlinear Dyn(2017)89: 937-953 DOI 10.1007/s11071-017-3493-2.
Effects of inspiratory muscle training in patients with chronic obstructive pulmonary disease after stratification by the degree of static hyperinflation
Authors: Sillen Maurice J.H. , Vaes Anouk W. , Groenen Miriam T.J. , Franssen Frits M.E. , Spruit Martijn A. 
Published: April 2022

Abstract
Background: Inspiratory muscle training (IMT) is a training method in patients with chronic obstructive pulmonary disease (COPD) to improve inspiratory muscle strength, exercise capacity and health status. It is unclear whether patients with different baseline degrees of static hyperinflation respond differentially to IMT as part of a pulmonary rehabilitation (PR) program. Therefore, the aim was to study the effects of IMT as an add-on on PR after stratification for baseline degrees of static hyperinflation.
Methods: In this single center retrospective study data were extracted between June 2013 and October 2020 of COPD patients. IMT was performed twice daily as part of a PR program. The primary outcome measure was maximal inspiratory mouth pressure (MIP). Secondary outcomes were endurance cycling and functional exercise capacity and disease-specific health status.
Results: After screening for eligibility and exclusion because of multiple reasons, 426 COPD patients were categorized into RV categories 50%-130%, 131%-165%, 166%-197%, 198%-234% and 235%-349%. In the whole sample, MIP, endurance exercise capacity and health status improved significantly. The change in 6MWD was higher in the lowest baseline degree of static hyperinflation (+39 (9-92) m) compared with the baseline highest degree of static hyperinflation (+11 (-18-54) m) (p<0.05).
Conclusions: IMT as part of a PR program in patients with COPD with different baseline degrees improved MIP irrespective of the degree of static lung hyperinflation. Improvement in functional exercise capacity was significantly higher in the group with the lowest degree of static hyperinflation compared with the patients with the highest degree of static hyperinflation.
Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells
Authors: Nishimura Kazuhiko , Kiriyama Naotake , Ogawa Kazuya , Inoue Reo , Haque Md. Anamul , Nakagawa Hiroshi 
Published: April 2022

Abstract
Arsenic is abundant in the environment and takes the form of trivalent and pentavalent arsenic compounds. Arsenite has been reported to both promote and suppress erythropoietin (EPO) production and autophagy induction. EPO production is involved in hematopoiesis and autophagy induction is involved in cytoprotection, both of which are thought to be cellular responses to arsenic stress. While there are reports that show the effects of EPO on autophagy induction, the relationship between EPO production and autophagy induction is unclear. Therefore, this study analyzed the effect of the pentavalent inorganic arsenic compound arsenate on EPO production and EPO-induced autophagy in HepG2 cells. Exposure of HepG2 cells to low-concentration arsenate was observed to increase EPO production and induced autophagy. Moreover, arsenate-induced ROS production was required to induce elevated EPO mRNA levels and autophagy. Both EPO production and autophagy induction contributed to protection from arsenate-induced cytotoxic stress. HepG2 cells expressed the EPO receptor and production of EPO by HepG2 cells acted in an autoregulatory manner to suppress autophagy induction. These results suggested that low-concentration arsenate promotes EPO production and autophagy induction in HepG2 cells, and the resultant EPO production contributes to cytoprotection of cultured cells via EPO receptor activation.
Feasibility of recruiting mother-infant dyads with mild-moderate depression to an art therapy painting group.
Authors: Moriarty Yvonne , O'Neill Claire , Robling Michael , Arroyo Carl , Owen Orion 
Published: April 2022

Abstract
Background: Depression in postnatal period is estimated to affect 10-20% of mothers and risks the long-term wellbeing of their infants. This study aimed to assess the feasibility of a novel dyadic mother-infant painting group for mothers with mild-moderate depression.Methods: Mothers (+infants) suffering with mild-moderate depression (measured using the Beck Depression Inventory-II) were recruited from two socioeconomically deprived urban areas in a UK city to join one of two 12-week painting groups in Autumn/Winter 2014. Groups were co-delivered by an art therapist, a specially trained health visitor and a nursery nurse. Data collection included: pre- and post-intervention interviews with mothers, session observations and focus groups with intervention staff and health visitor managers.Results: 11 mothers were approached and screened, six were referred for recruitment of which four consented and completed the pre-intervention interview. Two mothers attended almost every session and completed the post-intervention interview. Two mothers attended no sessions. One painting group was cancelled due to poor attendance. Health visitors found it difficult to determine participant eligibility and did not fully understand wider study aims, limiting recruitment. However, they identified as the appropriate professionals to approach mothers and suggested that more briefing and training sessions would be helpful. Delivering the groups in the intended non-directional approach challenged the interventionist health visitors’ normal nurturing approach. While initially apprehensive, attending mothers reported positive benefits including a sense of achievement. Relationship building with interventionists and other mothers was a key element to successful delivery. Some questions about the venue’s suitability were raised regarding the group size and babies’ age range should the group run at maximum capacity. Conclusions: Despite poor recruitment mothers benefited from session attendance and the intervention model showed some promise. However, further work is required to assess new recruitment methods for this population to enable further evaluation of the intervention model.Trial registration: N/A
Magnitude and factors associated with utilization of labor analgesia among obstetric caregivers at public hospitals in Sidama region, Southern Ethiopia
Authors: Alelgn Yitateku , kassa Andargachew , Abeje Abebaw , Tsegaye Brhan Tsegaye , Andargachew Andargachew Kassa , Belay Belay Amare , Abebaw Abebaw Abeje , Siyoum Melese Siyoum 
Published: April 2022

Abstract
Objective: The aim of the study was to assess utilization of labor analgesia and associated factors among obstetric caregivers working at public hospitals of Sidama region.Result: Among 346 respondents 143 (41.3%) 95%CI (36.1-46.2) were utilize labor analgesic, out of them 82(23.7%) were provide non-pharmacological labor analgesia, 60 (17.3%) were provide both pharmacological & non-pharmacological methods. Statically significant factors for utilization of labor analgesia were adequate knowledge [AOR =2.42, 95% CI (1.4-4.5)], higher qualification [AOR=3.9, 95%, CI (1.8-8.5)], availability of drugs [AOR=3.8, 95%CI (1.5-9.9)] and training on labor analgesia [AOR=3.3, 95%CI (1.8-6)]. The overall practice of labor analgesia was low. Adequate knowledge, high qualification, availability of drugs, and training on labor pain managements were factors significantly associated with the current utilization of labor analgesia in the study area.
Evidence for Psychometric Assessment of Resilience Scale (CD-RISC) and its role in Predicting Aggression among Iranian Athletic Adolescent Girls
Authors: Nooripour Roghieh , Hoseinian Simin , Vakili Yaghoob , Ghanbari Nikzad , Matacotta Joshua J. , Mozaffari Nazir , Ilanloo Hossein , Lavie Carl 
Published: April 2022

Abstract
The sport presents an opportunity for young people to experience the joys of success and cope with setbacks to develop resilient behaviors. However, there is a lack of clarity about how sport can cultivate resilience, particularly among adolescent girls. This study investigated the validation of the Resilience Scale (CD-RISC) and its role in predicting aggression in Iranian athletic adolescent girls. The method of the present study was cross-sectional. The population of this study was Iranian athletic adolescent girls, and 475 Iranian athletic adolescent girls were selected through the convenience sampling method. The participants completed the Resilience Scale (CD-RISC), Quality of Mindfulness, General Self-efficacy (GSE), Alexithymia, and Aggression Scale. The CD-RISC's psychometric properties were analyzed using confirmatory factor analysis (CFA), while reliability was tested using Cronbach’s alpha. Discriminant validity was measured by examining the relationship with alexithymia, and convergent validity was assessed with the quality of mindfulness and GSE. In addition, multiple regression analysis was conducted on the prediction of aggression by the CD-RISC subscales. The five-factor structure provided a good fit for the data. CD-RISC had significant negative associations with alexithymia, and there was a significant positive correlation between CD-RISC and Quality of Mindfulness, GSE. The results indicate that CD-RISC significantly predicts aggression in athletic adolescent girls. The CD-RISC has good validity for athletic adolescent girls in Iran and can be used in psychological evaluations in the Iranian context.
Design, Synthesis and Biological Evaluation of Amide derivatives of 1,3,4-oxadiazole-isoxazol-pyridine-benzimidazole as Anticancer Agents.
Authors: Devi Eeduri Ramya , Sreenivas Reddymasu , Malladi Sireesha , Nadh Ratnakaram Venkata , Rao Mandava Venkata Basaveswara , Rao Koya Prabhakara 
Published: April 2022

Abstract
We have designed and synthesized a series of novel amide derivatives of 1,3,4-oxadiazole-isoxazol-pyridine-benzimidazole (10a-j), and their structures are characterized by 1HNMR, 13CNMR and mass spectral data. The preliminary anticancer applications of these compounds are screened towards four types of human cancer cell lines including PC3 (prostate), A549 (lung), MCF-7 (breast) and DU-145 (prostate). The assay results revealed that many of the target compounds displayed remarkable anticancer activity. Among them, the compounds 10f, 10g, 10h and 10j are found to be more potent than rest of the compounds. In particularly, one compound 10f displayed most promising anticancer activity.
Experiences of Eating Difficulties in Siblings of People with Anorexia Nervosa: A Reflexive Thematic Analysis
Authors: Scutt Eleanor , Langdon-Daly Jasmin , Smithson Janet 
Published: April 2022

Abstract
BackgroundSiblings of people with Anorexia Nervosa (AN) have been found to experience strong emotions, changing family roles and poorer wellbeing as a consequence of witnessing the illness. These factors, combined with genetic influences, may put siblings at an increased risk of developing eating disorder psychopathology. This research aims to explore the experiences of siblings of people with AN who have had eating difficulties themselves and investigate issues that may be important to the development and prevention of eating difficulties in this population.MethodsThis qualitative study used a reflexive thematic analysis approach. Ten adults who had witnessed a sibling with AN and experienced eating difficulties themselves participated in semi-structured interviews. AnalysisParticipants’ own eating difficulties were affected by the specific experience of witnessing a sibling with AN through mealtimes becoming emotionally charged, an increased focus on body size and diet, and comparisons with their sibling. Difficult experiences, such as marital discord amongst parents were common, as was a difficulty in managing emotions. Changing family roles following their sibling’s illness could have influenced the development of eating difficulties, and limited participants’ ability to access support for their own difficulties. Systemic beliefs regarding the value of thinness were prevalent and influential. Possible protective factors, such as not wanting to become as unwell as a sibling with AN and an understanding of the negative consequences of AN, aided recovery.ConclusionsEating difficulties in siblings of people with AN may be influenced by competition for slimness, increased focus on diet and body size, and a need to manage difficult emotions. The disruption to social connections and a difficulty finding emotional support that may be experienced by people when a sibling develops AN may further influence susceptibility to eating difficulties. Further research is needed into the best ways to support siblings of people with AN.
Bubble velocimetry using the conventional and CNN-based optical flow algorithms
Authors: Park Hyungmin , Choi Daehyun , Kim Hyunseok 
Published: April 2022

Abstract
In the present study, we introduce new bubble velocimetry methods based on the optical flow, which were validated (compared) with the conventional particle tracking velocimetry (PTV) for various gas-liquid two-phase flows. For the optical flow algorithms, the convolutional neural network (CNN)-based models as well as the original schemes like the Lucas-Kanade and Farnebäck methods are considered. In particular, the CNN-based method was retrained (fine-tuned) using the synthetic bubble images produced by varying the density, diameter, and velocity distribution. While all models accurately measured the unsteady velocities of a single bubble rising with a lateral oscillation, the pre-trained CNN-based method showed the discrepancy in the averaged velocities in both directions for the dilute bubble plume. In terms of the fluctuating velocity components, the fine-tuned CNN-based model produced the closest results to that from PTV, while the conventional optical flow methods under-or overestimated them owing to the intensity assumption. When the void fraction increases much higher (e.g., over 10%) in the bubble plume, the PTV failed to evaluate the bubble velocities because of the overlapped bubble images and significant bubble deformation, which is clearly overcome by the optical flow bubble velocimetry. This is quite encouraging in experimentally investigating the gas-liquid two-phase flows of a high void fraction. Furthermore, the fine-tuned CNN-based model captures the individual motion of overlapped bubbles most faithfully while saving the computing time, compared to the Farnebäck method.