bluuebunny/crossref_metadata_2025_split huggingface dataset:
In-silico analysis unravels the structural and functional consequences of non-synonymous SNPs in the human IL-10 gene
Authors: Das Shuvo Chandra , Rahman Md. Anisur , Das Gupta Shipan 
Published: January 2022

Abstract
Background
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that affects different immune cells. It is also associated with the stimulation of the T and B cells for the production of antibodies. Several genetic polymorphisms in the IL-10 gene have been reported to cause or aggravate certain diseases like inflammatory bowel disease, rheumatoid arthritis, systemic sclerosis, asthma, etc. However, the disease susceptibility and abnormal function of the mutated IL-10 variants remain obscure.

Results
In this study, we used seven bioinformatics tools (SIFT, PROVEAN, PMut, PANTHER, PolyPhen-2, PHD-SNP, and SNPs&GO) to predict the disease susceptible non-synonymous SNPs (nsSNPs) of IL-10. Nine nsSNPs of IL-10 were predicted to be potentially deleterious: R42G, R45Q, F48L, E72G, M95T, A98D, R125S, Y155C, and I168T. Except two, all of the putative deleterious mutations are found in the highly conserved region of IL-10 protein structure, thus affecting the protein's stability. The 3-D structure of mutant proteins was modeled by project HOPE, and the protein–protein interactions were assessed with STRING. The predicted nsSNPs: R42Q, R45Q, F48L, E72G, and I168T are situated in the binding site region of the IL-10R1 receptor. Disruption of binding affinity with its receptor leads to deregulation of the JAK-STAT pathway and results in enhanced inflammation that imbalance in cellular signaling. Finally, Kaplan–Meier Plotter analysis displayed that deregulation of IL-10 expression affects gastric and ovarian cancer patients' survival rate. Thus, IL-10 could be useful as a potential prognostic marker gene for some cancers.

Conclusion
This study has determined the deleterious nsSNPs of IL-10 that might contribute to the malfunction of IL-10 protein and ultimately lead to the IL-10 associated diseases.
Comparison of radiation dose and its correlates between coronary computed tomography angiography and invasive coronary angiography in Northeastern Thailand
Authors: Aupongkaroon Phatraporn , Makarawate Pattarapong , Chaosuwannakit Narumol 
Published: January 2022

Abstract
Background
The number of coronary computed tomography angiography (CCTA) exams is steadily growing. A novel computed tomography (CT) system has been developed to increase image quality while lowering patient radiation. The radiation dose attributed to CCTA has received considerable attention, whereas the dose associated with invasive catheter angiography (ICA) has received less. This study aims to investigate the radiation exposure of CCTA in patients and compare it to ICA.

Results
The mean effective dose of CCTA was 2.88 ± 0.85 mSv which was significantly lower than the mean effective dose of ICA (5.61 ± 0.55 mSv), p < 0.0001. The effective dose of CCTA correlated with the weight, height, and BMI, while the effective dose of ICA was associated with patient weight and BMI. The radiation exposure from CCTA has been considerably reduced over the last ten years by almost 2.5 folds. The mean radiation dose from the newer generation CT used in 2019 was significantly lower than that of the single-source CT in 2010 (2.88 ± 0.85 mSv vs. 7.15 ± 3.4 mSv, p < 0.001).

Conclusions
CCTA allows evaluation of CAD with a significantly less effective radiation dose to patients than diagnostic ICA. There was a significant decrease in radiation dose from CCTA over time. Regular measurement of patient doses is an essential step to optimize exposure. It makes operators aware of their performance and allows comparisons with generally accepted practices.
Topiroxostat versus allopurinol in patients with chronic heart failure complicated by hyperuricemia: A prospective, randomized, open-label, blinded-end-point clinical trial
Authors: Sakuma Masashi , Toyoda Shigeru , Arikawa Takuo , Koyabu Yota , Kato Toru , Adachi Taichi , Suwa Hideaki , Narita Jun-ichi , Anraku Koetsu , Ishimura Kimihiko , Yamauchi Fumitake , Sato Yasunori , Inoue Teruo 
Published: January 2022

Background
The benefits of xanthine oxidase inhibitors to chronic heart failure (CHF) patients is controversial. We investigated the beneficial effects of a novel xanthine oxidoreductase inhibitor, topiroxostat, in patients with CHF and hyperuricemia (HU), in comparison to allopurinol.

Methods and results
The prospective, randomized open-label, blinded-end-point study was performed in 141 patients with CHF and HU at 4 centers. Patients were randomly assigned to either topiroxostat or allopurinol group to achieve target uric acid level ≤6.0 mg/dL. According to the protocol, 140 patients were followed up for 24 weeks. Percent change in ln (N-terminal-proB-type natriuretic peptide) at week 24 (primary endpoint) was comparable between topiroxostat and allopurinol groups (1.6±8.2 versus -0.4±8.0%; P = 0.17). In the limited number of patients with heart failure with reduced ejection fraction (HFrEF) (left ventricle ejection fraction <45%), ratio of peak early diastolic flow velocity at mitral valve leaflet to early diastolic mitral annular motion velocity (E/e’) decreased in topiroxostat group, but not in allopurinol group. Urinary 8-hydroxy-2’-deoxyguanosine and L-type fatty acid-binding protein levels increased and osmolality decreased significantly in allopurinol group, while these changes were less or absent in topiroxostat group. In allopurinol group HFrEF patients, additional to the increases in these urinary marker levels, urinary creatinine levels decreased, with no change in clearance, but not in topiroxostat group.

Conclusions
Compared with allopurinol, topiroxostat did not show great benefits in patients with CHF and HU. However, topiroxostat might have potential advantages of reducing left ventricular end-diastolic pressure, not worsening oxidative stress in proximal renal tubule, and renoprotection over allopurinol in HFrEF patients.
Development and psychometric properties of Iranian midwives job satisfaction instrument (MJSI): A sequential exploratory study
Authors: Direkvand-Moghadam Ashraf , Rashan Nasrin , Bahmani Mona , Taheri Safoura 
Published: January 2022

Background
Job satisfaction refers to a person’s attitude toward his/her job and its various aspects. Job satisfaction improves the quality of service and employees’ physical and mental health. The present study aimed to design a valid and reliable instrument to assess Iranian midwives job satisfaction instrument (MJSI).

Methods
This is a sequential exploratory study for tool design. This study in two phases; (qualitative and tool’s psychometric evaluation) was conducted in Ilam, Iran, 2019 years. In the first phase, a qualitative content analysis was carried out by in-depth and semi-structured individual interviews with 10 experts. Then, the pool of items extracted from the qualitative phase was completed by reviewing the existing texts and tools. The second phase of the study involved reducing the overlapping items and validating the tool. In order to investigate the construct validity, a cross sectional study was conducted with the participation of 121 midwives with census sampling. Data analysis was performed by SPSS-19 software using exploratory factor analysis and reliability tests (Cronbach’s alpha).

Results
In the qualitative phase and after reviewing the existing texts and tools by the research team, a 58-item questionnaire was developed and then entered into the psychometric phase. Then, the tool was finalized with five factors, including: 1) communication features, 2) professional features, 3) responsibility aspects, 4) physical-mental aspects and 5) social aspects, respectively. After the psychometric process, by removing the items in different stages, a specific questionnaire was developed to measure the midwives’ job satisfaction with 25 items which explained a total of 49.95% of the total variance. Reliability of the tool was approved by Cronbach’s alpha = 0.71 and test-retest with 2-weeks intervals, indicating an appropriate stability for the scale (ICC = 0.898).

Conclusion
The 25-item self-reporting midwives job satisfaction tool had acceptable validity and reliability. We recommend the use of this tool for evaluating the job satisfaction of midwives, as well as management and research purposes.
Improvements following multimodal pelvic floor physical therapy in gynecological cancer survivors suffering from pain during sexual intercourse: Results from a one-year follow-up mixed-method study
Authors: Cyr Marie-Pierre , Dostie Rosalie , Camden Chantal , Dumoulin Chantale , Bessette Paul , Pina Annick , Gotlieb Walter Henry , Lapointe-Milot Korine , Mayrand Marie-Hélène , Morin Mélanie 
Published: January 2022

Background
A large proportion of gynecological cancer survivors suffer from pain during sexual intercourse, also known as dyspareunia. Following a multimodal pelvic floor physical therapy (PFPT) treatment, a reduction in pain and improvement in psychosexual outcomes were found in the short term, but no study thus far has examined whether these changes are sustained over time.

Purpose
To examine the improvements in pain, sexual functioning, sexual distress, body image concerns, pain anxiety, pain catastrophizing, painful intercourse self-efficacy, depressive symptoms and pelvic floor disorder symptoms in gynecological cancer survivors with dyspareunia after PFPT, and to explore women’s perceptions of treatment effects at one-year follow-up.

Methods
This mixed-method study included 31 gynecological cancer survivors affected by dyspareunia. The women completed a 12-week PFPT treatment comprising education, manual therapy and pelvic floor muscle exercises. Quantitative data were collected using validated questionnaires at baseline, post-treatment and one-year follow-up. As for qualitative data, semi-structured interviews were conducted at one-year follow-up to better understand women’s perception and experience of treatment effects.

Results
Significant improvements were found from baseline to one-year follow-up on all quantitative outcomes (P ≤ 0.028). Moreover, no changes were found from post-treatment to one-year follow-up, supporting that the improvements were sustained at follow-up. Qualitative data highlighted that reduction in pain, improvement in sexual functioning and reduction in urinary symptoms were the most meaningful effects perceived by participants. Women expressed that these effects resulted from positive biological, psychological and social changes attributable to multimodal PFPT. Adherence was also perceived to influence treatment outcomes.

Conclusions
Findings suggest that the short-term improvements following multimodal PFPT are sustained and meaningful for gynecological cancer survivors with dyspareunia one year after treatment.
Long-term glycemic variability and the risk of mortality in diabetic patients receiving peritoneal dialysis
Authors: Afghahi Hanri , Nasic Salmir , Peters Björn , Rydell Helena , Hadimeri Henrik , Svensson Johan 
Published: January 2022

Background
The large amount of glucose in the dialysate used in peritoneal dialysis (PD) likely affects the glycemic control. The aim of this study was to investigate the association between HbA1c variability, as a measure of long-term glycemic variability, and the risk of all-cause mortality in diabetic patients with PD.

Methods
325 patients with diabetes and ESRD were followed (2008–2018) in the Swedish Renal Registry. Patients were separated in seven groups according to level of HbA1c variability. The group with the lowest variability was denoted the reference. The ratio of the standard deviation (SD) to the mean of HbA1c, HbA1c (SD)/HbA1c (mean), i.e. the coefficient of variation (CV), was defined as HbA1c variability. Hazard ratios (HR) and 95% confidence intervals (CI) were examined using Cox regression analyses.

Results
During follow-up, 170 (52%) deaths occurred. The highest mortality was among patients with the second highest HbA1c variability, CV≥2.83 [n = 44 of which 68% patients died]. In the multivariate analyses where lowest HbA1c variability (CV≤0.51) was used as the reference group, HbA1c CV 2.83–4.60 (HR 3.15, 95% CI 1.78–5.55; p<0.001) and CV> 4.6 (HR 2.48, 95% CI 1.21–5.11; p = 0.014) were associated with increased risk of death.

Conclusion
The high risk of all-cause mortality in patients with diabetes and PD increased significantly with elevated HbA1c variability, as measure of long-term glycemic control. This indicates that stable glycemia is associated with an improvement of survival; whereas more severe glycemic fluctuations, possibly caused by radical changes in dialysis regimes or peritonitis, are associated with a higher risk of mortality in diabetic patients with PD.
Distribution of introduced American mink in the Northern Apennine area (Central Italy)
Authors: Mori Emiliano , Bini Alessandro , Viviano Andrea , Bartolommei Paola , Mazza Giuseppe 
Published: January 2022

Abstract
The American mink Neovison vison is a widespread alien carnivore in Europe due to escapes from fur farms through this continent. Impacts by naturalized mink include competition with native species and predation of crayfish and small vertebrates. Therefore, an updated monitoring of mink distribution is required to design management plans. The American mink has been reported with an established population in North-Eastern Italy, in Sardinia, and two small nuclei respectively in the Central and in the Northern Apennine ridge. Available information for the latter dates back to early 1990s. In this note, we described the range expansion of the American mink population in the Northern Apennine ridge and we estimate the current distribution range and the extent of occurrence by using data collected from 2006 to 2021 through the Minimum Convex Polygon (MCP). We compared the current distribution range with that of 1990s. The American mink showed a remarkable range increase since its first escape in the Northern Apennines, thus requiring rapid actions to limit impacts on native biodiversity.
Alone With Our Thoughts: Investigation of Autonomy Supportive Framing as a Driver of Enjoyment During Quiet Time in Solitude
Authors: Nguyen Thuy-vy , Weinstein Netta , Deci Edward 
Published: January 2022

Sitting alone with one’s thoughts could foster a sense of rest and relaxation, yet many find this activity difficult. In two preregistered experiments (Study 1: n = 266, Study 2: n = 369), we focused on autonomy-supportive and controlling framings of solitude as drivers of motivation for solitude, positive experiences such as enjoyment and relaxation, negative experiences such as frustration and boredom, and negative thoughts like worries and rumination. In Study 1, we found support for one hypothesis that autonomy-supportive instructions to sit alone with thoughts led to greater autonomous motivation for solitude compared to controlling instructions that pressured participants to sit alone. However, the effect of instructions on autonomous motivation was trivial, with a smaller effect observed in Study 2. More importantly, we did not find evidence that our autonomy-supportive instructions meaningfully influenced self-reported measures of participants’ experiences with sitting alone with thoughts, nor both self-reported and behavioral measures intention to be in solitude again. Examination of null effects suggested that most differences between autonomy-supportive and controlling-instruction conditions were likely too small to be practically meaningful. However, some null findings in relation to excitement, relaxation, or frustration during sitting alone with thoughts were equivocal and required larger sample sizes to determine whether there was indeed an absence of effect. Consistent with findings reported by Nguyen et al. (2018), participants displayed drops in high-arousal types of affect and increases in low-arousal types of affect. Future research is needed to explore other factors that influence motivation for solitude and lead people to benefit from the regulatory effects of time spent alone.
Evaluación temporal y espacial en la calidad microbiológica del agua superficial: caso en un sistema de abastecimiento de agua para consumo humano en Costa Rica
Authors: Morales-Mora Eric , Reyes-Lizano Liliana , Barrantes-Jiménez Kenia , Chacón-Jiménez Luz 
Published: January 2022

[Introducción]: El agua para consumo es un determinante de la salud humana; particularmente cuando se obtiene de fuentes superficiales puede ser afectada por contaminantes microbiológicos causantes de enfermedades, los cuales a su vez son influenciados por las condiciones ambientales; sin embargo, estos efectos han sido poco estudiados en Costa Rica. [Objetivo]: Determinar la influencia de variables ambientales en las concentraciones de microorganismos patógenos e indicadores fecales en un sistema de abastecimiento de agua para consumo humano. [Metodología]: Se realizó un muestreo dos veces al mes durante un año. Se midió la concentración de Cryptosporidium sp., Giardia sp. colifagos somáticos y Escherichia coli. Se analizaron las condiciones espaciales mediante el uso de sistemas de información geográfica, y las condiciones climáticas de manera descriptivas. Para el análisis de datos se utilizaron técnicas para datos censurados y un modelo de regresión de Poisson para evaluar la influencia de la intensidad de precipitación sobre las concentraciones de microorganismos. [Resultados]: Se encuentran diferencias estacionales para colifagos somáticos y E. coli. Cryptosporidium sp. y Giardia sp. son más frecuentes cuando la intensidad de precipitación es débil, mientras que colifagos somáticos y E. coli presentan mayores concentraciones conforme aumenta la intensidad de precipitación. [Conclusiones]: Las cuatro microcuencas presentan vulnerabilidad por contaminación con respecto a sus condiciones espaciales y se determina que tanto la estacionalidad climática y la intensidad de la precipitación 24 h antes afectan de manera diferente a cada uno de los microorganismos en estudio, por lo cual, se recomienda su evaluación periódica de forma independientemente.
Application of gas chromatography-ion mobility spectrometry in the analysis of food volatile components
Authors: Yang Xuelian , Zhang Tianxin , Yang Dongdong , Xie Jianchun 
Published: January 2022

AbstractGas chromatography-ion mobility spectrometry (GC-IMS) is an emerging analytical technique that has the advantages of fast response, high sensitivity, simple operation, and low cost. The combination of the fast speed and resolution of GC with the high sensitivity of IMS makes GC-IMS play an important role in the detection of food volatile substances. This paper focuses on the basic principles and future development trend, and the comparative analysis of the functions, similarities and differences of GC-IMS, GC-MS and electronic nose in the detection of common volatile compounds. A comprehensive introduction to the main application of GC-IMS in food volatile components: fingerprint identification of sample differences and detection of characteristic compounds. On the basis of perfecting the spectral library, GC-IMS will have broad development prospects in food authentication, origin identification, process optimization and product classification, especially in the analysis and identification of trace volatile food flavor substances.
Counseling Services in Health Care for Covid-19 Patients
Authors: Hidayanti Ema , Mintarsih Widayat , Ma’arif Syamsul , Nurrochmah Fiyan Tika 
Published: January 2022

<span>COVID-19 patients face physical and psychological problems. Physical complaints are usually fever, cough, fatigue, and shortness of breath, while psychological problems are anxiety, fear, and stress during isolation. Referring to the problem, the health care for patients with COVID-19 should not only for handling physical problems but also psycho-social-religious problems. One way to deal with the psychological problem is to provide counseling services conducted in referral hospitals for COVID-19 patients in Adiyathma general hospital and Wongosonegoro general hospital of Semarang. The health care of COVID-19 patients in the hospital pays attention to the psychological problems through counseling services conducted by psychologists. This study was a qualitative study with a phenomenological approach to explore counseling services in health care for COVID-19 patients. The subjects of this study were COVID-19 patients who were Muslim and underwent isolation in hospitals. The results showed that counseling services provide based on doctors' recommendations or the patient's request due to psychological problems such as anxiety, fear, and adaptation disorders. Counseling practices were conducted using common methods such as relaxation, psychoeducation, client center, and CBT (cognitive behavior therapy), combined with religious methods (Islam) by inviting patients to get closer to Allah through prayer and dhikr. </span>
PENERAPAN METODE SERVQUAL DAN AHP SEBAGAI ANALISIS KUALITAS PELAYANAN TERHADAP KEPUASAN KONSUMEN BENGKEL AKENA MALANG
Authors: Efendi Mukhlisin , Harianto Wahyudi , Nugraha Danang Aditya 
Published: January 2022

Penelitian ini bertujuan untuk mengukur kualitas pelayanan dimana untuk mengetahui kesenjangan dan faktor yang mempengaruhi kepuasan konsumen terhadap kualitas pelayanan yang diperoleh sesuai dengan harapan konsumen. Untuk itu perlu adanya analisis dengan metode Servqual dibantu dengan software SPSS 22 dan dilakukan perhitungan bobot dari setiap dimensi dengan metode Analytical Hierarchy Process (AHP) dibantu dengan Microsoft Excel. Dari hasil penelitian ini diperoleh Servqual terbobot dimana pada dimensi tangible dan emphaty bernilai negatif sedangkan pada dimensi reability, responsiveness dan assurance bernilai positif. Dan dengan pembobotan AHP diperoleh 5 atribut kualitas pelayanan yang perlu ditingkatkan yaitu pada atribut A6 (-0,18), atribut A9 (-0,17), atribut A8 (-0,16), atribut A4 (-0,13), dan Atribut E4 (-0,06).