bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular analysis of NUT‐positive poromas and porocarcinomas identifies novel break points of YAP1::NUTM1 fusions
Authors: Snow Justin T. , Georgantzoglou Natalia , Green Donald C. , Parra Ourania , LeBlanc Robert E. , Yan Shaofeng , Sriharan Aravindhan , Momtahen Shabnam , Winnick Kimberley N. , Dimonitsas Emmanouil , Stavrianos Spiros , Lakiotaki Eleftheria , Korkolopoulou Penelope , Revelos Kyriakos , Guo Ruifeng , Linos Konstantinos 
Published: June 2022

AbstractBackgroundPoromas, and their malignant counterparts, porocarcinomas, harbor recurrent translocations involving YAP1‐MAML2, YAP1‐NUTM1, and infrequently WWTR1‐NUTM1; YAP1‐NUTM1 being the most common in porocarcinomas. NUT immunohistochemistry (IHC) can be used to identify NUTM1‐translocated tumors. This study sought to investigate potential novel NUTM1‐fusion partners among NUT IHC‐positive poromas and porocarcinomas.MethodsThirteen NUT IHC‐positive poroid tumors (four poromas and nine porocarcinomas) were identified within a multi‐institutional international cohort. Next‐generation sequencing (NGS) assessed for NUTM1 fusion partners.ResultsNGS detected a NUTM1 fusion in 12 of 13 cases: YAP1‐NUTM1 (11/12 cases) and WWTR1‐NUTM1 (1/12 cases). Two of the cases (2/12) with NUTM1 fusion were not called by the NGS algorithm but had at least one read‐spanning YAP1‐NUTM1 break point upon manual review. A NUTM1 fusion was not identified in one case; however, the sample had low RNA quality. The following fusion events were identified: YAP1 exon 4::NUTM1 exon 3 in six cases, YAP1 exon 6::NUTM1 exon 2 in one case, YAP1 exon 3::NUTM1 exon 3 in three cases, WWTR1 exon 3::NUTM1 exon 3 in one case, and YAP1 exon 8::NUTM1 exon 3 fusion in one case.ConclusionWhile no novel NUTM1 fusion partners were identified within our cohort, 12 of 13 cases had discoverable NUTM1 fusions; YAP1‐NUTM1 fusion was detected in 11 cases (92%) and WWTR1‐NUTM1 in 1 case (8%). These data corroborate findings from other recent investigations and further substantiate the utility of NUT IHC in diagnosing a subset of poroid neoplasms. In addition, two of our cases harbored fusions of YAP1 exon 6 to NUTM1 exon 3 and YAP1 exon 8 to NUTM1 exon 2, which have not been reported before in poroid neoplasms and indicate novel break points of YAP1.
Impact of the hepatoselective glucokinase activator TTP399 on ketoacidosis during insulin withdrawal in people with type 1 diabetes
Authors: Klein Klara R. , Boeder Schafer C. , Freeman Jennifer L. R. , Dunn Imogene , Dvergsten Chris , Madduri Supradeep , Giovannetti Erin R. , Valcarce Carmen , Buse John B. , Pettus Jeremy H. 
Published: June 2022

AbstractAimsTo determine the effect of TTP399, a hepatoselective glucokinase activator, on the risk of ketoacidosis during insulin withdrawal in individuals with type 1 diabetes (T1D).Materials and methodsTwenty‐three participants with T1D using insulin pump therapy were randomized to 800 mg TTP399 (n = 12) or placebo (n = 11) for 7 to 10 days. After the treatment period, an insulin withdrawal test (IWT) was performed, during which insulin pumps were removed to induce ketogenesis. The IWT was stopped after 10 hours or if blood glucose reached >399 mg/dL [22.1 mmol/L], if beta‐hydroxybutyrate (BHB) was >3.0 mmol/L, or for patient discomfort. The primary endpoint was the proportion of participants who reached BHB concentrations of 1 mmol/L or greater.ResultsDuring the 7‐ to 10‐day treatment period, mean fasting plasma glucose was significantly reduced ( −27.6 vs. −4.4 mg/dL [−1.5 vs. −0.2 mmol/L]; P = 0.03) and there were fewer adverse events, including hypoglycaemia, in the TTP399‐treated arm. During the IWT, no differences were observed between TTP399 and placebo in mean serum BHB concentration, mean duration of IWT, or BHB at termination of IWT. However, serum bicarbonate was numerically higher and urine acetoacetate was quantitatively lower in the TTP399‐treated participants. As a result of higher bicarbonate values, none of the TTP399‐treated participants met the prespecified criteria for diabetic ketoacidosis (DKA), defined as BHB >3 mmol/L and serum bicarbonate <18 mEq/L, compared to 42% of placebo‐treated participants.ConclusionsWhen used as an adjunctive therapy to insulin, TTP399 improves glycaemia without increasing hypoglycaemia in individuals with T1D. During acute insulin withdrawal, TTP399 did not increase BHB concentrations and decreased the incidence of DKA.
RBP‐J deficiency promoted the proliferation and differentiation of CD133‐positive cells in both in vitro and in vivo studies
Authors: Ye Xin , Li Mengyi , Bian Wei , Wu Anting , Zhang Ting , Li Junwei , Zhou Peng , Cui Huairui , Ding Yu‐Qiang , Liao Min , Sun Chenyou 
Published: June 2022

AbstractAlthough Notch signalling pathway could control the proliferation and differentiation of neural stem cells (NSCs), it is largely unknown about the effect of Notch signalling pathway on the neurogenesis of CD133‐positive cells. By using the primary cultured ependymal cells and the transgenic mouse, we found that CD133 immunoreactivity was exclusively localized in the ependymal layer of ventricles; moreover, most CD133‐positive cells were co‐labelled with Nestin. In addition, recombination signal binding protein J (RBP‐J), a key nuclear effector of Notch signalling pathway, was highly active in CD133‐positive cells. CD133‐positive cells can differentiate into the immature and mature neurons; in particular, the number of CD133‐positive cells differentiating into the immature and mature neurons was significantly increased following the deficiency or interference of RBP‐J in vivo or in vitro. By using real‐time qPCR and Western blot, we found that RBP‐J and Hes1 were downregulated, whereas Notch1 was upregulated in the expression levels of mRNAs and proteins following the deficiency or interference of RBP‐J. These results demonstrated RBP‐J deficiency promoted the proliferation and differentiation of CD133‐positive cells. Therefore, we speculated that RBP‐J could maintain CD133‐positive cells in the characteristics of NSCs possibly by regulating Notch1/RBP‐J/Hes1 pathway. It will provide a novel molecular insight into the function of RBP‐J as well as facilitate a future investigation of CD133‐positive cells with respect to their potential application in neurodegenerative disorder.
Evidence for origin of lavender foal syndrome among Egyptian Arabian horses in Egypt
Authors: AbouEl Ela Nahla Hussien , El Araby Iman E. , Saleh Ayman A. , Abd El‐fattah Amir H. , Hagag Naglaa M. , Brooks Samantha A. , Radwan Mohamed A. , Kalbfleisch Ted 
Published: June 2022

AbstractBackgroundLavender foal syndrome (LFS) is a fatal hereditary condition that is inherited in an autosomal recessive pattern. This detrimental mutation is more common in Arabian foals of Egyptian origin than foals from other bloodlines. Heterozygous horses are carriers of the LFS trait and appear normal, while recessive homozygous foals died shortly after birth due to serious complications. In Egypt, in 2014, an Egyptian foal died after manifestations of neurological signs and abnormal coat colour as LFS signs. Therefore, it is important to identify LFS carriers in the population of Arabian horses in Egypt and to encourage improvement of the Arabian horse industry in Egypt by constructing a breeding system based on genetic background in order to avoid mating between carriers and reduce financial losses from deaths of affected foals.ObjectivesTo establish a PCR‐based test for detecting the MYO5A gene mutation causing LFS in the registered Arabian horse population in Egypt prior to breeding.Study designCross sectional survey (n = 170) plus targeted sampling (n = 30).MethodsA total of 200 samples were collected from an Arabian farm in Egypt and some of them were traced for LFS based on the farm records. The LFS genotypes were identified using the PCR‐RFLP technique, fragment analysis followed by sequence analysis.ResultsThe overall mutated allele and genotype frequencies (N/L) were 0.08 and 16%, respectively.ConclusionThe observed frequency of heterozygotes suggests foals affected with LFS will be produced among Arabian horses in Egypt. Therefore, screening of the entire population for this mutation should be undertaken in the breeding program.
Comparison of Malassezia spp. colonization between human skin exposed to high‐ and low‐ambient air pollution
Authors: Chueachavalit Chutinan , Meephansan Jitlada , Payungporn Sunchai , Sawaswong Vorthon , Chanchaem Prangwalai , Wongpiyabovorn Jongkonnee , Thio Hok Bing 
Published: June 2022

AbstractThe skin microbiota is essential for human health; altered skin microbiome colonization and homeostasis may be associated with several inflammatory skin conditions and other inflammatory diseases. Malassezia spp. are commensal fungi commonly found on the human skin, and they also play a pathogenic role in various skin diseases. It is hypothesized that the exposure of human skin to air pollution might be associated with Malassezia spp. colonization. The aim of this study was to compare Malassezia spp. colonization on healthy human skin between people living in two major cities in Thailand with different air qualities: one city with highly polluted ambient air and the other with less polluted air. Skin microbiome samples from 66 participants were collected using swabbing and scraping techniques. The skin fungal composition was analysed using high‐throughput sequencing based on internal transcribed spacer 2 (ITS2) rDNA. A significant difference was found in alpha and beta diversities and the relative abundance of fungal profiles between the groups. The relative abundance of Malassezia spp. was found to be significantly higher in the highly polluted area than in the less polluted area. This study demonstrates that high‐ambient air pollution may alter Malassezia spp. colonization on healthy human skin, which could lead to dysbiosis of the cutaneous ecosystem and eventually result in some skin disorders.
Microalgal food sources greatly improve macroinvertebrate growth in detritus‐based headwater streams: Evidence from an instream experiment
Authors: Labed‐Veydert Tiphaine , Danger Michael , Felten Vincent , Bec Alexandre , Laviale Martin , Cellamare Maria , Desvilettes Christian 
Published: June 2022

Abstract

In forested headwater streams, inconspicuous food resources such as epilithic microalgae can play a major role owing to their content of long chain polyunsaturated fatty acids (PUFAs) that are essential for macroinvertebrate development. Yet, the use of these resources and their consequences for consumers and life‐history traits remain scarcely studied, especially for non‐herbivorous taxa.

Using instream mesocosms, we aimed to understand how two macroinvertebrate species, a shredder detritivore (Gammarus pulex) and a scraper (Rhithrogena semicolorata), use available organic resources under light versus shaded conditions (high and low amounts of phototrophic biofilm, respectively). We specifically focused on the origin of carbon (C) and essential lipids (PUFAs, sterols) assimilated by the two species and the effects of these compounds on their survival and growth.

When autotrophic biofilms were available (mesocosms exposed to light), both species experienced significantly higher growth than in mesocosms placed in the dark. In Rhithrogena nymphs, the survival and imago emergence rates were positively affected by access to autotrophic biofilms.

Using stable isotope analysis (δ13C and δ15N), we demonstrated that under dark conditions both macroinvertebrates assimilated C of detrital origin. Under light conditions, most of the C assimilated by R. semicolorata was derived from autotrophic biofilms and around 11% from fine detrital particles (FPOM). Gammarus pulex derived part of its C from detrital sources (mainly FPOM) and from autotrophic biofilms.

The results of isotopic analyses (δ13C) on fatty acids (FAs) and sterols showed that the scraper Rhithrogena was entirely dependent on autotrophic biofilms to meet its dietary FAs and sterol requirements. In contrast, detrital sources were quantitatively important for G. pulex both in terms of C and sterol supply, irrespective of the conditions tested. For these consumers, microalgae seemed to be a complementary food source, but yet essential to cover a large part of their requirements in long‐chain PUFAs.

This study clearly confirmed the ecological importance of autotrophic biofilms for two functional feeding groups of macroinvertebrates in headwater streams providing new insights on the trophic origin of sterols and long‐chain PUFAs in their diet.
Non‐native plant invasion can accelerate global climate change by increasing wetland methane and terrestrial nitrous oxide emissions
Authors: Bezabih Beyene Bahilu , Li Junjie , Yuan Junji , Dong Yanhong , Liu Deyan , Chen Zengming , Kim Jinhyun , Kang Hojeong , Freeman Chris , Ding Weixin 
Published: June 2022

AbstractApproximately 17% of the land worldwide is considered highly vulnerable to non‐native plant invasion, which can dramatically alter nutrient cycles and influence greenhouse gas (GHG) emissions in terrestrial and wetland ecosystems. However, a systematic investigation of the impact of non‐native plant invasion on GHG dynamics at a global scale has not yet been conducted, making it impossible to predict the exact biological feedback of non‐native plant invasion to global climate change. Here, we compiled 273 paired observational cases from 94 peer‐reviewed articles to evaluate the effects of plant invasion on GHG emissions and to identify the associated key drivers. Non‐native plant invasion significantly increased methane (CH4) emissions from 129 kg CH4 ha−1 year−1 in natural wetlands to 217 kg CH4 ha−1 year−1 in invaded wetlands. Plant invasion showed a significant tendency to increase CH4 uptakes from 2.95 to 3.64 kg CH4 ha−1 year−1 in terrestrial ecosystems. Invasive plant species also significantly increased nitrous oxide (N2O) emissions in grasslands from an average of 0.76 kg N2O ha−1 year−1 in native sites to 1.35 kg N2O ha−1 year−1 but did not affect N2O emissions in forests or wetlands. Soil organic carbon, mean annual air temperature (MAT), and nitrogenous deposition (N_DEP) were the key factors responsible for the changes in wetland CH4 emissions due to plant invasion. The responses of terrestrial CH4 uptake rates to plant invasion were mainly driven by MAT, soil NH4+, and soil moisture. Soil NO3−, mean annual precipitation, and N_DEP affected terrestrial N2O emissions in response to plant invasion. Our meta‐analysis not only sheds light on the stimulatory effects of plant invasion on GHG emissions from wetland and terrestrial ecosystems but also improves our current understanding of the mechanisms underlying the responses of GHG emissions to plant invasion.
More than a feeling? What does compassion in healthcare ‘look like’ to patients?
Authors: Baguley Sofie I. , Pavlova Alina , Consedine Nathan S. 
Published: June 2022

AbstractObjectiveCompassion is important to patients and their families, predicts positive patient and practitioner outcomes, and is a professional requirement of physicians around the globe. Yet, despite the value placed on compassion, the empirical study of compassion remains in its infancy and little is known regarding what compassion ‘looks like’ to patients. The current study addresses limitations in prior work by asking patients what physicians do that helps them feel cared for.MethodsTopic modelling analysis was employed to identify empirical commonalities in the text responses of 767 patients describing physician behaviours that led to their feeling cared for.ResultsDescriptively, seven meaningful groupings of physician actions experienced as compassion emerged: listening and paying attention (71% of responses), following‐up and running tests (11%), continuity and holistic care (8%), respecting preferences (4%), genuine understanding (2%), body language and empathy (2%) and counselling and advocacy (1%).ConclusionThese findings supplement prior work by identifying concrete actions that are experienced as caring by patients. These early data may provide clinicians with useful information to enhance their ability to customize care, strengthen patient–physician relationships and, ultimately, practice medicine in a way that is experienced as compassionate by patients.Public ContributionThis study involves the analysis of data provided by a diverse sample of patients from the general community population of New Zealand.
Analysis of protein denaturation, and chemical visualisation, of frozen grass carp surimi containing soluble soybean polysaccharides
Authors: Gao Wenhong , Wu Xinru , Ye Ruisen , Zeng Xin‐an , Brennan Margaret A. , Brennan Charles S. , Ma Ji 
Published: June 2022

SummaryThe mechanism of protein denaturation of frozen surimi enriched with soluble soybean polysaccharides (SSPS) was investigated. Near‐infrared (NIR) and hyperspectral imaging (HSI) technology were used to predict protein denaturation. The fresh grass carp surimi was divided into four groups with SSPS additions of 0%, 1%, 3% and 5%, respectively, which were frozen and stored at −18 °C. Samples were examined after 0, 1, 2, 4 and 8 weeks, for salt‐soluble protein content, total SH, NIR hyperspectral image and Raman spectrum features. The results showed that addition of SSPS decelerated protein degradation. After 8 weeks of storage, the 5% SSPS addition maintained the highest salt‐soluble protein content, while for total SH, 3% and 5% SSPS addition had similar effect. Raman spectra illustrated that SSPS had the ability of maintaining an α‐helix content, as well as decreasing the exposure of polar groups, and reducing the oxidation of SH group into disulphide bonds. NIR spectra showed that the overall reflectance of frozen surimi increased with the increase in SSPS. Two partial least squares regression (PLSR) models were established after pretreatment of HSI spectral statistics and selection of characteristic wavelengths. The distribution maps were finally generated based on the simplified PLSR models.
Glycaemic response of pseudocereal‐based gluten‐free food products: a review
Authors: Punia Bangar Sneh , Sharma Nitya , Singh Arashdeep , Phimolsiripol Yuthana , Brennan Charles S. 
Published: June 2022

SummaryRapid digestion and absorption of carbohydrates have become a health issue (high glycaemic index, GI), which can be a matter of greater concern when consumed in large quantities. Depending upon the influence of carbohydrates on the blood sugar levels, GI classifies carbohydrates (on a scale of 100) as low (<55), medium (55–70) and high (>70) GI foods. Among the pseudocereals, chia seed possesses relatively lower GI (28.53), as compared to buckwheat (52.35), amaranth (47.65) and quinoa (61.50). Consumers now prefer foods with a high GI over the ones with low GI to prevent various metabolic alterations. Celiac disease is a lifelong disorder prevalent worldwide and can only be controlled by following a strict lifelong gluten‐free diet. Therefore, pseudocereals could be a potential alternate for low GI food and developing gluten‐free food products, including bread, cookies, noodles and pasta. This review synthesises the recently published literatures on pseudocereals as a lowering GI and healthy food option. This review also gives insights into developing pseudocereals as a potential and novel ingredient for gluten‐free food applications and the latest research conducted worldwide.
Critical research and entrepreneurship: A cross‐disciplinary conceptual typology
Authors: Dey Pascal , Fletcher Denise , Verduijn Karen 
Published: June 2022

AbstractCritical perspectives of entrepreneurship have gained increasing traction over the last two decades. The transformative potential of critical research resides in challenging some of entrepreneurship research's epistemological, ontological and theoretical assumptions, with a view to offering a range of alternatives. Critical research in entrepreneurship has remained fragmented, however, due to its heterogeneous theoretical lineages and compartmentalized and niche interests. Addressing this situation, our objective is to intensify the space of critique in entrepreneurship research by offering a theoretically informed typology that delineates different manifestations of ‘criticalness’. Our overarching contribution is to advance a typology distinguishing four ideal types of critical entrepreneurship research based on evaluative emphases (referred to as ‘valence’) and the meta‐theoretical assumptions informing its critical operation (called ‘paradigmatic orientation’). By demonstrating the variegated political, ethical and ideological interests and preoccupations that critical studies serve within different management sub‐disciplines, the typology provides a conceptual vocabulary for making sense of and synthesizing critical perspectives across scholarly boundaries. Also, it helps to reposition understandings of critique as gestures of negativity by stimulating a greater appreciation of the generative potential of critique and the theoretical and philosophical possibilities that this can bring to our scholarly community.
A survey of stakeholders' perceived importance of health indicators and subgroup analyses to inform the Canadian clinical practice guideline for managing paediatric obesity
Authors: Gehring Nicole D. , Johnston Bradley C. , Birken Catherine , Buchholz Annick , Cooper Jenny , Erdstein Julius , Fitzpatrick‐Lewis Donna , Hadjiyannakis Stasia , Hamilton Jill , Hatanaka Dawn , Henderson Mélanie , Lebel Tracy , Moore Sarah A. , Morrison Katherine M. , Page Shawn , Pearce Nicole , Sebastianski Megan , Sherifali Diana , St‐Pierre Julie , Zenlea Ian , Ball Geoff D. C. 
Published: June 2022

SummaryObjectiveTo assess stakeholder ratings of health indicators and subgroup analyses in systematic reviews used to update the Canadian Clinical Practice Guideline for Managing Paediatric Obesity.MethodsStakeholders (caregivers of children with obesity and Clinical Practice Guideline Steering Committee members) completed an online survey between April 2020 and March 2021. Participants rated importance of health indicators and subgroup analyses for behavioural and psychological, pharmacotherapeutic, and surgical interventions for managing paediatric obesity from not important to critically important using Grading, Recommendations, Assessment, Development and Evaluation criteria.ResultsNo health indicators or subgroup analyses were rated not important by the 30 caregivers and 17 Steering Committee members. Across intervention types, stakeholders rated anxiety, depression, health‐related quality of life, serious adverse events, plus age and weight status subgroups as critically important.ConclusionStakeholder ratings will inform data reporting and interpretation to update Canada's Clinical Practice Guideline for Managing Paediatric Obesity.