bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influenza infection recruits distinct waves of regulatory T cells to the lung that limit lung resident IgA+ B cells
Authors: Sjaastad Louisa E. , Owen David L. , Joo Sookyong , Knutson Todd P. , O’Connor Christine H. , McCluskey Braedan , LaRue Rebecca S. , Langlois Ryan A. , Farrar Michael A. 
Published: September 2022

AbstractThe role of regulatory T cells (Tregs) in limiting responses to pathogens in tissues remains poorly described. We used scRNA-Seq and a newly generatedFoxp3-lineage reporter line (Foxp3-iDTRmice) to track Tregs in the lungs and peripheral blood following infection with influenza virus. Few Tregs of any type were found in the lung at steady-state. Following influenza infection Tregs expressing a strong interferon-stimulated gene signature (ISG-Tregs) appeared by day 3, peaked by day 7, and largely disappeared by day 21 post-infection. A second diverse wave of tissue-repair-like Tregs (TR-Tregs) appeared by day 10 and were maintained through day 21 post-infection. These two distinct Treg subsets had different gene expression patterns and distinct TCR repertoires. To establish the role of Tregs during influenza infection, we acutely ablated Tregs at day 6 post-infection; this resulted in a significant increase in IgA+ B cells in the lung. To determine whether distinct Tregs subsets could also be observed in response to respiratory viral infections in humans we analyzed scRNA-Seq datasets of patients with COVID-19. Peripheral blood from healthy human volunteers had multiple Treg subsets defined by unique gene expression patterns, but few ISG-Tregs. In contrast, two distinct Tregs subsets were expanded in COVID-19 patients - ISG-Tregs and IL32 expressing Tregs (16-fold and 2-fold increased, respectively). ISG-Tregs were present at significantly higher levels in patients with mild versus severe COVID-19, while IL32 expressing Tregs showed the opposite pattern. Thus, the Treg response to respiratory viruses in humans is also diverse and correlates with disease outcome.
Chaperones Hsc70 and Hsp70 Play Distinct Roles in Bocaparvovirus Minute Virus of Canines Infection
Authors: Guo Jianhui , Sun Jinhan , Yan Yan , Ji Kai , Hei Zhiping , Zeng Liang , Xu Huanzhou , Ren Xiang , Sun Yuning 
Published: September 2022

ABSTRACTMinute virus of canines (MVC) belongs to the genusBocaparvovirusand reproduces rapidly in its permissive cells Walter Reed/3873D (WRD). The crosstalk between MVC and WRD is poorly characterized in terms of molecular requirements and mechanisms. Here, we identified two novel cellular proteins Hsc70 and Hsp70 that interact with both NS1 and VP2 via mass spectrometry (MS), co-immunoprecipitation (Co-IP) and confocal immunofluorescence assays (IFA). Hsp70 was upregulated upon MVC infection. Respective silencing of Hsc70 and Hsp70 led to contrasting results at nearly every stage of MVC life cycle, including virus entry, transcription, translation, replication and production. Strikingly, transfection with low and high dose of pFlag-Hsp70 contributed to opposing impacts on viral protein levels and virus production possibly through a ubiquitin-dependent manner, indicating that MVC is quite sensitive to the levels of Hsp70. Treatment with quercetin and VER155008, two Hsp70 family inhibitors, both significantly decreased viral replication and particle levels. Together, these results illustrated that both Hsc70 and Hsp70 are involved in MVC life cycle, and targeting to Hsp70 family may represent a novel anti-MVC mechanism.
Comparative transcriptomics reveals human-specific cortical features
Authors: Jorstad Nikolas L. , Song Janet H.T. , Exposito-Alonso David , Suresh Hamsini , Castro Nathan , Krienen Fenna M. , Yanny Anna Marie , Close Jennie , Gelfand Emily , Travaglini Kyle J. , Basu Soumyadeep , Beaudin Marc , Bertagnolli Darren , Crow Megan , Ding Song-Lin , Eggermont Jeroen , Glandon Alexandra , Goldy Jeff , Kroes Thomas , Long Brian , McMillen Delissa , Pham Trangthanh , Rimorin Christine , Siletti Kimberly , Somasundaram Saroja , Tieu Michael , Torkelson Amy , Ward Katelyn , Feng Guoping , Hopkins William D. , Höllt Thomas , Keene C. Dirk , Linnarsson Sten , McCarroll Steven A. , Lelieveldt Boudewijn P. , Sherwood Chet C. , Smith Kimberly , Walsh Christopher A. , Dobin Alexander , Gillis Jesse , Lein Ed S. , Hodge Rebecca D. , Bakken Trygve E. 
Published: September 2022

AbstractHumans have unique cognitive abilities among primates, including language, but their molecular, cellular, and circuit substrates are poorly understood. We used comparative single nucleus transcriptomics in adult humans, chimpanzees, gorillas, rhesus macaques, and common marmosets from the middle temporal gyrus (MTG) to understand human-specific features of cellular and molecular organization. Human, chimpanzee, and gorilla MTG showed highly similar cell type composition and laminar organization, and a large shift in proportions of deep layer intratelencephalic-projecting neurons compared to macaque and marmoset. Species differences in gene expression generally mirrored evolutionary distance and were seen in all cell types, although chimpanzees were more similar to gorillas than humans, consistent with faster divergence along the human lineage. Microglia, astrocytes, and oligodendrocytes showed accelerated gene expression changes compared to neurons or oligodendrocyte precursor cells, indicating either relaxed evolutionary constraints or positive selection in these cell types. Only a few hundred genes showed human-specific patterning in all or specific cell types, and were significantly enriched near human accelerated regions (HARs) and conserved deletions (hCONDELS) and in cell adhesion and intercellular signaling pathways. These results suggest that relatively few cellular and molecular changes uniquely define adult human cortical structure, particularly by affecting circuit connectivity and glial cell function.
Reduced insulin signalling in neurons induces sex-specific health benefits
Authors: Baghdadi Maarouf , Nespital Tobias , Mesaros Andrea , Buschbaum Sandra , Withers Dominic J. , Grönke Sebastian , Partridge Linda 
Published: September 2022

AbstractReduced activity of the insulin/IGF signalling (IIS) network extends healthspan and lifespan in mammals and possibly humans. Loss of the Irs1 gene increases survival in mice and causes tissue-specific changes in gene expression. However, the tissues underlying IIS mediated longevity are currently unknown. Here we measured survival and healthspan in male and female animals lacking Irs1 activity specifically in the liver, muscle, fat and brain. Tissue-specific loss of IRS1 did not increase survival, suggesting that lack of Irs1 in more than one tissue is required for lifespan extension. Furthermore, loss of Irs1 in liver, muscle and fat did not improve health at old age. In contrast, loss of neuronal Irs1 increased energy expenditure, locomotion and insulin sensitivity, specifically in old males. Neuronal loss of IRS1 also caused male-specific mitochondrial dysfunction, activation of Atf4 and metabolic adaptations consistent with an activated integrated stress response at old age. Thus, we identified a male-specific brain signature of ageing in response to reduced IIS associated with improved health outcomes at old age.
The genomic basis of hybrid male sterility in Ficedula flycatchers
Authors: Segami J. Carolina , Mugal Carina F , Cunha Catarina , Bergin Claudia , Schmitz Monika , Semon Marie , Qvarnström Anna 
Published: September 2022

AbstractIdentifying genes involved in genetic incompatibilities causing hybrid sterility or inviability is a long-standing challenge in speciation research, especially in studies based on natural hybrid zones. Here we present the first high-probability candidate genes for hybrid male sterility in birds by using a combination of whole genome sequence data, histology sections of testis and single cell transcriptomics of testis samples from male pied-, collared-, and hybrid flycatchers. We reveal failure of meiosis in hybrid males and propose candidate genes involved in genetic incompatibilities causing this failure. Based on identification of genes with non-synonymous fixed differences between the two species and revealing miss-expression patterns of these genes across the various stages of hybrid male spermatogenesis we conclude aberrant chromosome segregation and/or faulty chromatin packing. A lower proportion of spermatids produced by hybrid males implies that a proportion of the aberrant spermatids undergo apoptosis. Finally, we report an overrepresentation of Z-linkage of the revealed candidate incompatibility genes. Our results challenge the assumption that speciation processes are driven by fast evolving genes by showing that a few changes in genes with highly conserved and central functions may quickly ensure reproductive isolation through post-zygotic isolation.
In-vivoantidiarrheal activity: From the crude extract and solvent fractions ofRhamnus prinoides(Rhamnaceae) leaves
Authors: Mengie Ayele Teklie , Abebe Endeshaw Chekol , Muche Zelalem Tilahun , Agidew Melaku Mekonnen , Yimer Yohannes Shumet , Addis Getu Tesfaw , Baye Nega Dagnaw , Kassie Achenef Bogale , Alemu Muluken Adela , Yiblet Tesfagegn Gobezie , Tiruneh Gebrehiwot Ayalew , Dagnew Samuel Berihun , Moges Tilaye Arega , Tadesse Tesfaye Yimer , Zelalem Amein Ewnetei 
Published: September 2022

AbstractObjectivesThe inherent toxicities of the drugs urge the search for alternative drugs that are safe and effective. Therefore, the objective of the study is to evaluate thein-vivoanti-diarrheal activity of crude extract and solvent fractions ofRhamnus prinoidesleaves.MethodsThe Leaves ofRhamnus prinoideswere macerated using absolute methanol and then fractionated. Forin-vivoantidiarrheal activity evaluation of the crude extract and solvent fraction, castor oil-induced diarrhea, castor oil-induced anti-enteropolling, and intestinal transit models were used. One-way analysis of variance was used to analyze the data, followed by a Tukey post-test. The standard and negative control groups were treated with loperamide and 2% tween 80 respectively.ResultsA significant reduction in the frequency of wet stools and watery content of diarrhea, intestinal motility, intestinal fluid accumulation, and delaying the onset of diarrhea as compared with controls were observed in mice treated with 200 mg/kg and 400 mg/kg ME. However the effect increased dose-dependently, and the 400 mg/kg ME produced a comparable effect with the standard drug in all models. Amongst the solvent fractions, n-BF significantly delayed the time of diarrheal onset and reduced the frequency of defecation, and intestinal motility at doses of 200mg/kg and 400mg/kg. Furthermore, the maximum percentage inhibition of intestinal fluid accumulation was observed in mice treated with 400 mg/kg n-BF (p<0.01; 61.05%).ConclusionThe results of this study showed that crude leaves extract and solvent fractions ofRhamnus prinoideshad significant anti-diarrheal activity, providing scientific support for its traditional use as a diarrhea treatment.
Effects of nitrous oxide and ketamine on the prefrontal cortex in mice: a comparative study
Authors: Rozov Stanislav , Saarreharju Roosa , Khirug Stanislav , Storvik Markus , Rivera Claudio , Rantamäki Tomi 
Published: September 2022

AbstractNitrous oxide (N2O; laughing gas) has recently been reported as a putative rapid-acting antidepressant, but little is known about the underlying mechanisms. We performed transcriptomics,in situhybridization, and electrophysiological studies to examine the potential shared signatures induced by 1 h inhalation of 50% N2O and a single subanesthetic dose of ketamine in the medial prefrontal cortex (mPFC) in adult mice. Both treatments similarly affected the transcription of several negative regulators of mitogen-activated protein kinases (MAPKs), namely, dual specificity phosphatases. The effects were primarily located in the pyramidal cells. Notably, the overall effects of N2O on mRNA expression were much more prominent and widespread compared to ketamine. Ketamine caused an elevation of the spiking frequency of putative pyramidal neurons and increased gamma activity (30–100 Hz) of cortical local field potentials. However, N2O produced no such effects. Spiking amplitudes and spike-to-local field potential phase locking of putative pyramidal neurons and interneurons in this brain area showed no uniform changes across treatments. Thus, this study characterized the electrophysiological and transcriptome-wide changes in mPFC triggered by exposure to N2O and compared them with those caused by the rapid-acting antidepressant ketamine in terms of both the direction of their regulation and localization.
Methodological Paper What counts as “good” qualitative accounting research? Researchers' perspectives on assessing and proving research quality
Authors: Steccolini Ileana 
Published: September 2022

PurposeThis study explores the everyday experiences of researchers in assessing their own and others' research, highlighting what “good” qualitative accounting research is from their perspectives.Design/methodology/approachThe analysis is based on interviews with accounting scholars from the UK, Germany, Italy, Spain and Australia, with diverse ethnic background and methodological preferences.FindingsInterviewees pointed to a plurality of practical, and to some extent tacit, ways in which they demonstrate and assess the quality of research, concerning “contribution”, “consistency” and “confidence”, with generalizability being seen as more controversial and difficult to attain. In general, interviewees highlighted the underlying ambiguity on what constitutes good research in the qualitative accounting community, contrasting it to the perceived stronger clarity to be found in the quantitative accounting community. This was seen as potentially strengthening the positions of “gatekeepers” in the accounting communities, and encouraging conformance and “signaling” behaviors, at the risk of hampering innovation.Originality/valueThe main critical issues affecting qualitative research quality highlighted by interviewees concern the engagement with the world of practice, and with theory and literature, the importance of accounting for the analysis of qualitative data and for the messiness of the underlying process, and the implicit search for compliance with editors' and community's expectations and conventions. These findings suggest the need to continue debating how to assess the quality of qualitative research in everyday activities, and reflect on how to promote acceptance and openness to pluralism, in scientific communities, as well as in data collection, analysis, in the theorizing, and in connecting epistemology and methodology.
Dividend regulation and cost stickiness: evidence from a quasi-natural experiment
Authors: Chen Liangyin , Huang Jun , Hu Danqi , Chen Xinyuan 
Published: September 2022

PurposeThis paper aims to examine the effect of dividend regulation on cost stickiness (i.e. the asymmetric change in firm expense between sales increase and sales decrease) and explore the underlying mechanism.Design/methodology/approachBased on the quasi-natural experiment of the Guideline for Dividend Policy of Listed Companies issued by the Shanghai Stock Exchange (SSE) in 2013, the authors employ a difference-in-difference model to investigate the impact of dividend regulation on cost stickiness.FindingsThe authors find that the cost stickiness of treatment group firms has decreased significantly when compared with control group firms after the dividend regulation. Moreover, this effect is more pronounced among firms in lower marketization regions, in lower competition industries and those with less analyst coverage and lower cash flow levels. Further analyses show that dividend regulation reduces the cost stickiness of firms by mitigating agency problems. Finally, the conclusion holds after several robust tests, including controlling for firm fixed effect, propensity score matching (PSM), placebo test and reconstruction of expense variable.Originality/valueThis paper confirms that dividend regulation serves an important role in corporate governance, which reduces firms' agency costs and thereby decreases cost stickiness. The conclusions shed light on the dividend policies of listed companies and capital market regulation in the future.
On general uncertainty data and general uncertainty variable for reliability growth analysis of major aerospace equipment
Authors: Liu Sifeng , Tang Wei 
Published: September 2022

PurposeThe purpose of this paper is to explore new ways and lay a solid foundation to solve the problem of reliability growth analysis of major aerospace equipment with various uncertainty data through propose new concepts of general uncertainty data (GUD) and general uncertainty variable (GUV) and build the operation system of GUVs.Design/methodology/approachThe characteristics of reliability growth data of major aerospace equipment and the limitations of current reliability growth models have been analyzed at first. The most commonly used uncertainty system analysis methods of probability statistics, fuzzy mathematics, grey system theory and rough set theory have been introduced. The concepts of GUD and GUV for reliability growth data analysis of major aerospace equipment are proposed. The simplified form of GUV based on the “kernel” and the degree of uncertainty of GUV is defined. Then an operation system of GUVs is built.Findings(1) The concept of GUD; (2) the concept of GUV; (3) The novel operation rules of GUVs with simplified form.Practical implicationsThe method exposed in this paper can be used to integrate complex reliability growth data of major aerospace equipment. The reliability growth models based on GUV can be built for reliability growth evaluation and forecasting of major aerospace equipment in practice. The reliability evaluation example of a solid rocket motor shows that the concept and idea proposed in this paper are feasible. The research of this paper opens up a new way for the analysis of complex uncertainty data of reliability growth of major aerospace equipment. Moreover, the operation of GUVs could be extended to the case of algebraic equation, differential equation and matrix which including GUVs.Originality/valueThe new concepts of GUD and GUV are given for the first time. The novel operation rules of GUVs with simplified form were constructed.
Enhancing online-to-offline delivery efficiency facilitated by Industry 4.0: a personnel configuration perspective
Authors: Dai Hongyan , Wen Yan , Zhou Weihua , Tong Tingting , Xu Xun 
Published: September 2022

PurposeThe overuse and scarcity of resources emphasize the importance of the circular economy. The technology facilitated by Industry 4.0 stimulates the implementation of the circular economy that aims to reduce resource use and enhance operational efficiency. This study focuses on enhancing delivery efficiency in an online-to-offline (O2O) context from an Industry 4.0 technology-facilitated personal configuration perspective, that is, comparing in-house and crowdsourced delivery efficiency in China's O2O on-demand food delivery context.Design/methodology/approachThe authors collect 128,152 orders from 38 restaurants of an online restaurant chain in China. The authors adopt multiple regression analysis to examine the delivery efficiency gap between in-house and crowdsourced deliverymen and the determinants of this efficiency gap.FindingsThe findings of this study reveal that crowdsourced deliverymen exhibit higher delivery efficiency, in terms of a shorter delivery time, than in-house deliverymen. In addition, the authors find that platforms providing monetary incentives or implementing late delivery penalties enlarge this efficiency gap. Furthermore, the authors show that external factors, such as working on weekends and bad weather conditions, contribute to the narrowing of this performance efficiency.Practical implicationsThe study's findings suggest that platforms should use advanced technologies facilitated by Industry 4.0 to optimize their personnel configuration to enhance their delivery efficiency and reduce carbon emissions. The effective approaches include using financial incentives and improving working schedules.Originality/valueThe authors' findings contribute to the online fulfillment literature by focusing on delivery efficiency in the O2O context from the Industry 4.0 technology-facilitated personnel configuration perspective. The authors examine how internal and external factors moderate the performance efficiency between these two types of deliverymen.
“Should I adopt Islamic banking services?” Factors affecting non-Muslim customers’ behavioral intention in the Malaysian context
Authors: Mustapha Norhudayati , Mohammad Jihad , Quoquab Farzana , Salam Zarina Abdul 
Published: September 2022

PurposeThe acceptance of Islamic banking services by non-Muslim customers is not promising in the Malaysian context. Thus, this study aims to examine the effect factors that may motivate this customer group to adopt the Islamic banking services in the Malaysian context.Design/methodology/approachA self-administered questionnaire was used to collect data through google docs from 188 respondents. Structural equation modelling partial least squares (PLS-SEM) was used to analyze the hypothetical model of this study.FindingsThe result of this study found that knowledge about Islamic banking exerts a positive effect on non-Muslim beliefs (attitudinal, control and norm). Moreover, this study found a significant relationship between attitude, subjective norm, perceived behavioral control with non-Muslim Intention towards Islamic banking services. Furthermore, the mediating effect of attitude, subjective norm and perceived behavioral control was supported.Originality/valueThis is a pioneer study that contributes to the consumer behavior literature by extending the theory of planned behavior by incorporating knowledge in the model. In addition, this study develops comparatively new relationships, practically the mediation role of beliefs (attitudinal, control and norm) between knowledge and intention.
A Seventh-day Adventist farm community in Tanzania and vegetarianism as a social practice
Authors: Lestar Tamas 
Published: September 2022

PurposeThis paper is the outcome of an empirical research on a Seventh-day Adventist farm in Tanzania. The author investigated the role of Christian spirituality in switching to and maintaining vegetarian practices. Dietary change is proposed in the sustainability literature as a crucial trajectory to mitigate the impacts of climate change. The purpose of this paper is to explore the links between spirituality and climate-friendly dining in a localised Christian context and discuss their significance further for wider society.Design/methodology/approachSocial practice theory (SPT) provided the tools to explore, empirically, the dynamic development of dietary practice within the farm community and its relation to the outside world; according to SPT, following the main building blocks of practices, namely materials, competences and meanings (cognitive or emotional), helps to understand the evolution of practices in society.FindingsFindings show that the spiritual element of the community's dietary practice is key in maintaining commitment to vegetarianism, despite the rationale focussing exclusively on human health.Social implicationsExpanding the rationale to animal compassion and environmental concerns could enhance the stabilisation of the practice within and beyond the community's realms.Originality/valueThe research showcases, probably for the first time, how a localised vegetarian practice may be linked to broader societal developments and policymaking through the application of SPT.