bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between influenza vaccination and SARS-CoV-2 infection and its outcomes: systematic review and meta-analysis
Authors: Jiang Binshan , Huang Qiangru , Jia Mengmeng , Xue Xinai , Wang Qing , Yang Weizhong , Feng Luzhao 
Published: November 2022

AbstractBackground:World Health Organization recommends that influenza vaccines should benefit as much of the population as possible, especially where resources are limited. Corona virus disease 2019 (COVID-19) has become one of the greatest threats to health systems worldwide. The present study aimed to extend the evidence of the association between influenza vaccination and COVID-19 to promote the former.Methods:In this systematic review, four electronic databases, including the Cochrane Library, PubMed, Embase, and Web of Science, were searched for related studies published up to May 2022. All odds ratios (ORs) with 95% confidence intervals (CIs) were pooled by meta-analysis.Results:A total of 36 studies, encompassing 55,996,841 subjects, were included in this study. The meta-analysis for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection provided an OR of 0.80 (95% CI: 0.73–0.87). The statistically significant estimates for clinical outcomes were 0.83 (95% CI: 0.72–0.96) for intensive care unit admission, 0.69 (95% CI: 0.57–0.84) for ventilator support, and 0.69 (95% CI: 0.52–0.93) for fatal infection, while no effect seen in hospitalization with an OR of 0.87 (95% CI: 0.68–1.10).Conclusion:Influenza vaccination helps limit SARS-CoV-2 infection and severe outcomes, but further studies are needed.Registration:PROSPERO, CRD 42022333747.
Multi-time point imaging in 99mTc-pertechnetate scintigraphy of ectopic gastric mucosa in duplication cysts: value-added services
Authors: Khurana Aditi , Garg Sumit , Ravindra Shubha G. , Kumar Yadav Devendra , Jain Vishesh , Bajpai Minu , Agarwala Sandeep , Bal Chandrashekhar , Kumar Rakesh 
Published: November 2022

Objectives
The aim of our study was to describe the scintigraphic patterns of 99mTc-pertechnetate uptake in patients who were referred to the department of nuclear medicine for evaluating and diagnosing ectopic gastric mucosa in foregut and midgut duplication cysts.

Materials and methods
This hospital-based, retrospective cum prospective research spans a period of 8 years from April 2014 to January 2022. Previous hospital medical records were analyzed and subsequently, a database was prepared which included the age, sex, clinical indication of a 99mTc-pertechnetate scan, and the planar and SPECT-computed tomography (CT) imaging findings. Postoperative histopathological reports were available for 21 patients. Dynamic and planar static imaging was performed. We included SPECT-CT in suspected duplication cysts to increase the sensitivity and specificity which is a tradeoff for a small amount of additional radiation exposure. A total of 69 patients were subjected to a 99mTc-pertechnetate scan for suspected foregut or midgut duplication cysts. All were subjected to dynamic planar and delayed static images up to 24 h or until focal uptake of radiotracer was noted which corroborated the anatomical findings, whichever was earlier. SPECT-CT was performed along with the planar study in 31 patients which confirmed the findings. Previously performed CT scans were used for anatomical correlation in the remaining ones

Results
Duplication cysts were localized in a total of 28 patients (19 foregut duplication cysts and 12 small bowel duplications – 3 patients had dual duplication cysts, both foregut, and midgut). Forty-one patients had no scintigraphic evidence of ectopic gastric mucosa. Of these 69 patients, histopathological diagnosis was available for 21 patients (22 lesions). The report was concordant with the scan findings in 15 patients (16 lesions) and 6 patients showed discordance in histopathological diagnosis and scan findings.

Conclusion
In conclusion, multi-time point imaging is the key to diagnosing ectopic gastric mucosa of various sizes and in various locations. An abnormal radiotracer uptake in dynamic sequences, even before the appearance of the stomach in the region of the small bowel is indicative of intestinal duplication, and delayed radiotracer visualization in the region of the thorax is characteristic of intrathoracic foregut duplication cyst.
An Unexpected Death Due to a Subclinical Pheochromocytoma After an Induced Abortion
Authors: Lupariello Francesco , Mirri Federica , Davico Laura , Di Vella Giancarlo 
Published: November 2022

Abstract
Deaths due to hypertensive crisis can be unexplained because of the impossibility to identify—before the decease—the specific cause of the crisis. In these cases, autopsy and microscopic examinations are needed. It can happen that the request to conduct these examinations can be submitted to forensic pathologists by prosecutors to ascertain medical malpractice claims. Indeed, we report the case of a 36-year-old woman who died after a minimally invasive medical procedure because of the occurrence of a severe hypertensive crisis. After death, the woman's relatives raised formal complaint for medical malpractice. For this reason, the prosecutor ordered to perform an autopsy. Examining the abdominal cavity, the forensic pathologists identified the left adrenal gland as expanded; in correspondence with organ's medulla, there was a gray and noncapsulated mass. Immunohistochemistry was performed: the microscopic samples were diffusely positive for chromogranin A, suggesting the diagnosis of a subclinical pheochromocytoma. In light of the above, medical malpractice was excluded. This case highlights that forensic pathologists should be aware that, in case of unexpected deaths due to hypertensive crisis, adrenal glands should be always accurately evaluated, performing specific immunohistochemistry study to exclude the presence of a pheochromocytoma.
MONITORING OF PULMONARY EDEMA USING ULTRASOUND RADIOFREQUENCY SIGNAL
Authors: Kwon Hyuksool , Jo You Hwan , Lee Jae Hyuk , Hwang Ji Eun , Park Inwon , Kim Seonghye , Jang Dong-Hyun , Kim Doyun , Chang Hyunglan 
Published: November 2022

ABSTRACT

Objectives: Excessive accumulation of extravascular lung water impairs respiratory gas exchange and results in respiratory distress. Real-time radiofrequency signals of ultrasound can continuously and quantitatively monitor excessive lung water. This study aims to evaluate the availability of continuous real-time quantitative pulmonary edema monitoring using ultrasound radiofrequency signals and compare it with Pao
2 (partial pressure of arterial oxygen)/Fio
2 (fraction of inspired oxygen) (PF) ratio, conventional lung ultrasound, and the Hounsfield unit of chest computed tomography. Methods: Male Yorkshire pigs (40.5 ± 0.5 kg) were anesthetized and mechanically ventilated. A balanced crystalloid was administered to induce hydrostatic pulmonary edema. Three different infusion rates of 2, 4, and 6 mL/kg per minute were tested to determine the infusion rate for the appropriate swine model. The chest computed tomography and ultrasonography with radiofrequency signals were taken every 5 min during the full inspiration. The ultrasonography scans with radiofrequency signals were measured at the intercostal space where the line crossing the two armpits and the right anterior axillary line intersected. Results: The infusion rate of fluid for the pulmonary edema model was determined to be 6 mL/kg per minute, and a total of four pigs were tested at an injection rate of 6 mL/kg. The adjusted R
2 values of regression analysis between the radiofrequency signal and computer tomography Hounsfield score were 0.990, 0.993, 0.988, and 0.993 (all P values <0.05). All radiofrequency signal changes preceded changes in PF ratio or lung ultrasound changes. The area under the receiver operating characteristic curve of the radiofrequency signal for predicting PF ratio <300 was 0.88 (95% confidence interval, 0.82–0.93). Conclusion: We evaluated ultrasound radiofrequency signals to assess pulmonary edema in a swine model that can worsen gradually and showed that quantitative ultrasound radiofrequency signal analysis could assess pulmonary edema and its progression before PF ratio or lung ultrasound changes.
Evaluating the Impact of Assessment Metrics for Simulated Central Venous Catheterization Training
Authors: Brown Dailen C. , Gonzalez-Vargas Jessica M. , Tzamaras Haroula M. , Sinz Elizabeth H. , Ng Philip K. , Yang Michael X. , Adhikary Sanjib D. , Miller Scarlett R. , Moore Jason Z. 
Published: November 2022

Introduction
Performance assessment and feedback are critical factors in successful medical simulation-based training. The Dynamic Haptic Robotic Trainer (DHRT) allows residents to practice ultrasound-guided needle insertions during simulated central venous catheterization (CVC) procedures while providing detailed feedback and assessment. A study was performed to examine the effectiveness of the DHRT in training the important skills of needle tip tracking and aspiration and how these skills impact procedural complications in simulated CVC.

Methods
The DHRT data were collected for 163 residents at 2 hospitals for 6 simulated needle insertions. Users were given automated feedback on 5 performance metrics, which measure aspiration rate, arterial punctures, punctures through and through the vein, loss of access to the vein, and successful access to the vein. Aspiration rates and tip tracking rates were analyzed to determine their significance in preventing CVC complications and improving performance.

Results
Tip tracking rates higher than 40% were 2.3 times more likely to result in successful venous access than rates less than 10%. Similarly, aspiration rates higher than 80% were 2.6 times more likely to result in successful venous access than rates less than 10%. Proper tip tracking and aspiration both reduced mechanical complications. Resident performance improved for all metrics except tip tracking.

Conclusions
Proper tip tracking and aspiration both reduced complications and increased the likelihood of success. However, the skill of tip tracking was not effectively learned through practice without feedback. Therefore, ultrasound-guided needle-based procedures, including CVC, can be improved by providing specific feedback to users on their ultrasound usage to track needle insertions.
The genetic and phenotypic correlates of neonatal Complement Component 3 and 4 protein concentrations with a focus on psychiatric and autoimmune disorders
Authors: Borbye-Lorenzen Nis , Zhu Zhihong , Agerbo Esben , Albiñana Clara , Benros Michael E. , Bian Beilei , Børglum Anders D , Bulik Cynthia M. , Goldtsche Debost Jean-Christophe Philippe , Grove Jakob , Hougaard David M. , McRae Allan F , Mors Ole , Mortensen Preben Bo , Musliner Katherine L. , Nordentoft Merete , Petersen Liselotte V. , Privé Florian , Sidorenko Julia , Skogstrand Kristin , Werge Thomas , Wray Naomi R , Vilhjálmsson Bjarni J. , McGrath John J. 
Published: November 2022

AbstractThe complement system, including complement components 3 and 4 (C3, C4), traditionally has been linked to innate immunity. More recently, complement components have also been implicated in brain development and the risk of schizophrenia. Based on a large, population-based case-cohort study, we measured the blood concentrations of C3 and C4 in 68,768 neonates. We found a strong correlation between the concentrations of C3 and C4 (phenotypic correlation = 0.65,P-value < 1.0×10−100, genetic correlation = 0.38,P-value = 1.9×10−35). A genome-wide association study (GWAS) for C4 protein concentration identified 36 independent loci, 30 of which were in or near the major histocompatibility complex on chromosome 6 (which includes theC4gene), while six loci were found on six other chromosomes. A GWAS for C3 identified 15 independent loci, seven of which were located in theC3gene on chromosome 19, and eight loci on five other chromosomes. We found no association between (a) measured neonatal C3 and C4 concentrations, imputed C4 haplotypes, or predictedC4gene expression, with (b) schizophrenia (SCZ), bipolar disorder (BIP), depression (DEP), autism spectrum disorder, attention deficit hyperactivity disorder or anorexia nervosa diagnosed in later life. Mendelian randomisation (MR) suggested a small positive association between higher C4 protein concentration and an increased risk of SCZ, BIP, and DEP, but these findings did not persist in more stringent analyses. Evidence from MR supported causal relationships between C4 concentration and several autoimmune disorders: systemic lupus erythematosus (SLE, OR and 95% confidence interval, 0.37, 0.34 – 0.42); type-1 diabetes (T1D, 0.54, 0.50 - 0.58); multiple sclerosis (MS, 0.68, 0.63 - 0.74); rheumatoid arthritis (0.85, 0.80 - 0.91); and Crohn’s disease (1.26, 1.19 - 1.34). A phenome-wide association study (PheWAS) in UK Biobank confirmed that the genetic correlates of C4 concentration were associated a range of autoimmune disorders including coeliac disease, thyrotoxicosis, hypothyroidism, T1D, sarcoidosis, psoriasis, SLE and ankylosing spondylitis. We found no evidence of associations between C3 versus mental or autoimmune disorders based on either MR or PheWAS. In general, our results do not support the hypothesis that C4 is causally associated with the risk of SCZ (nor several other mental disorders). We provide new evidence to support the hypothesis that higher C4 concentration is associated with lower risks of autoimmune disorders.
Risk of SARS-CoV-2 reinfection is time- and variant-dependant, France, January 2021 to August 2022
Authors: Auvigne Vincent , Schaeffer Justine , Boudon Thibault , Tamandjou Cynthia , Figoni Julie , du Châtelet Isabelle Parent , Bernard-Stoecklin Sibylle 
Published: November 2022

AbstractSince the emergence of Omicron, reinfections with SARS-CoV-2 have been rising. We estimated the risk of SARS-CoV-2 reinfection in the widely vaccinated French population, from January to August 2022. At nine weeks post-infection, the relative risk of reinfection, primary infection with pre-Delta variants being the reference group, was estimated at 0.43 [95%CI 0.40-0.47] if the primary infection was attributed to Delta, 0.21 [95%CI 0.19-0.24] with BA.1 and 0.17 [95% CI 0.15-0.18] with BA.2, and rapidly waned overtime. After a BA.1 primary infection the protection was similar against BA.2 or BA.4/5 reinfection.
Diversity and Distribution of Arrow Worms, Zooplankton Along the Mangaluru and Udupi Coast, Karnataka
Authors: Daggula Narshivudu , Ramachandra Naik A.T. , Lakshmipathi M. T , Somashekara S. R , Manjappa N , Suresh T 
Published: November 2022

ABSTRACTThe present study was done to understand the abundance and species diversity of arrow worms, zooplankton in relation with hydrographical parameters and the spatial and temporal variations for a period of 16 months from October 2020 to March 2022. Monthly sampling was carried out at selected stations of coastal waters with average depth of 8m. The qualitative analyses revealed the presence of seventeen different groups of zooplankton. copepods, cladocerans are dominant group in zooplankton followed by chaetognaths, during the study period seven species of chaetognaths recorded viz,Sagitta enflata, Sagitta robusta, Sagitta bedotii, Sagitta bipunctata, Sagitta ferox, Sagitta planktonis, andSagitta elegans. Among them,Sagitta enflatawas the dominant species followed byS.bedotiiandS. robusta. The richness, evenness and diversity of chaetognaths varied in the different stations and the maximum and minimum values also varied between stations, but in the most of the stations the minimum values were observed in the post-monsoon season and the highest values ranged between January to March. On comparison of the present abundance of chaetognaths with previous study, it was observed that the density of chaetognaths decreased compared to previous studies.
Efficacy and safety of therapeutic HPV vaccines to treat CIN 2/CIN 3 lesions: a systematic review and meta-analysis of phase II/III clinical trials
Authors: Khalil Ahmadaye Ibrahim , Zhang Li , Muwonge Richard , Sauvaget Catherine , Basu Partha 
Published: November 2022

AbstractObjectivesWe aims to assess the efficacy and safety of therapeutic HPV vaccines to treat cervical intraepithelial neoplasia of grade 2 or 3 (CIN2/3).DesignThis study is a systematic review and meta-regression that follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses recommendations.Data sourcesPubMed, Embase, Web of Science, Global Index Medicus and CENTRAL Cochrane were searched up January 31, 2022.Eligibility criteriaPhase II/III studies reporting the efficacy of therapeutic vaccines to achieve regression of CIN2/3 lesions were included.Data extraction and synthesisTwo independent reviewers extracted data, evaluated study quality. A random-effect model was used to pool the proportions of regression and/or HPV clearance.Results12 trials met the inclusion criteria. Out of the total 734 women receiving therapeutic HPV vaccine for CIN 2/3, 414 regressed to normal/CIN1 with the overall proportion of regression of 0.54 (95%CI: 0.39, 0.69) for vaccinated group. Correspondingly, 166 women receiving placebo only achieving the pooled normal/CIN1 regression of 0.27 (95%CI: 0.20, 0.34). When only including two-arm studies, the regression proportion of the vaccine group was higher than that of control group (relative risk (RR): 1.52, 95%CI: 1.14, 2.04). Six studies reported the efficacy of the therapeutic vaccines to clear high-risk human papillomavirus (hrHPV) with the pooled proportion of hrHPV clearance of 0.42 (95%CI: 0.32, 0.52) for the vaccine group and 0.17 (95%CI: 0.11, 0.26) for the control group and the RR of 2.03 (95%CI: 1.30, 3.16). Similar results were found regarding HPV16/18 clearance. No significant unsolicited adverse events have been consistently reported.ConclusionsThe efficacy of the therapeutic vaccines in the treatment of CIN2/3 was modest. Besides, the implementation issues like feasibility, acceptability, adoption, and cost-effectiveness need to be further studied.PROSPERO registration numberCRD42020189617Strengths and limitations-This systematic review and meta-analysist on the clinical efficacy and safety of therapeutic human papillomavirus (HPV) vaccines to treat cervical intraepithelial neoplasia of grade 2 or 3 lesions based on phase II/III trials.-Notre recherche documentaire a impliqué une recherche approfondie d’essais cliniques, en utilisant un large éventail de termes de recherche et sans limitation de langue, de pays ou de date.-We had to combine studies that were variable in case selection (e.g., some included CIN 2/3 lesions that were positive for HPV 16/18 only while others included lesions associated with any high-risk HPV types).-Some of the studies did not have a control arm, which made it difficult to ascertain whether the responses observed were due to natural regression alone.
An RFX transcription factor regulated ciliogenesis in the progenitors of choanoflagellates and animals
Authors: Coyle Maxwell C. , Tajima Adia M. , Leon Fredrick , Choksi Semil P. , Yang Ally , Espinoza Sarah , Hughes Timothy R. , Reiter Jeremy F. , Booth David S. , King Nicole 
Published: November 2022

AbstractLittle is known about the origins of the transcriptional modules that coordinate cell-type specific functions in animals. The controlled expression of one cellular feature – the cilium – was likely critical during early animal evolution. Two key transcription factors, RFX and FoxJ1, coordinate ciliogenesis in animals but are absent from the genomes of most other ciliated eukaryotes, raising the question of how the transcriptional regulation of ciliogenesis has evolved. To reconstruct the evolution of the RFX/FoxJ1 transcriptional module and its role in the regulation of ciliogenesis, we investigated RFX and FoxJ1 function in one of the closest living relatives of animals, the choanoflagellateSalpingoeca rosetta. Targeted disruption of theS. rosettaRFX homologcRFXaresulted in delayed cell proliferation and aberrant ciliogenesis, marked by the collapse and resorption of nascent cilia. Ciliogenesis genes andfoxJ1were significantly down-regulated incRFXamutants, consistent with a pre-animal ancestry for this transcriptional module. We also found that cRFXa protein preferentially binds to a sequence motif that is enriched in the promoters ofS. rosettaciliary genes and matches the sequence motif bound by animal RFX proteins. These findings suggest that RFX coordinated ciliogenesis before the divergence of animals and choanoflagellates, and that the deployment of this module may have provided a mechanism to differentiate ciliated and non-ciliated cell types in early animal evolution.
Bispecific antibodies combine breadth, potency, and avidity of parental antibodies to neutralize sarbecoviruses
Authors: Radić Laura , Sliepen Kwinten , Yin Victor , Brinkkemper Mitch , Capella-Pujol Joan , Schriek Angela I. , Torres Jonathan L. , Bangaru Sandhya , Burger Judith A. , Poniman Meliawati , Bontjer Ilja , Bouhuijs Joey H. , Gideonse David , Eggink Dirk , Ward Andrew B. , Heck Albert J. R. , Van Gils Marit J. , Sanders Rogier W. , Schinkel Janke 
Published: November 2022

AbstractSARS-CoV-2 mutational variants evade humoral immune responses elicited by vaccines and current monoclonal antibody (mAb) therapies. Novel antibody-based treatments will thus need to exhibit broad neutralization against different variants. Bispecific antibodies (bsAbs) combine the specificities of two distinct antibodies into one antibody taking advantage of the avidity, synergy and cooperativity provided by targeting two different epitopes. Here we used controlled Fab-arm exchange (cFAE), a versatile and straightforward method, to produce bsAbs that neutralize SARS-CoV and SARS-CoV-2 variants, including Omicron and its subvariants, by combining potent SARS-CoV-2-specific neutralizing antibodies with broader but less potent antibodies that also neutralize SARS-CoV. We demonstrate that the parental IgG’s rely on avidity for their neutralizing activity by comparing their potency to bsAbs containing one irrelevant “dead” Fab arm. We used single particle mass photometry to measure formation of antibody:spike complexes, and determined that bsAbs increase binding stoichiometry compared to corresponding cocktails, without a loss of binding affinity. The heterogeneous binding pattern of bsAbs to spike (S), observed by negative-stain electron microscopy and mass photometry provided evidence for both intra- and inter-spike crosslinking. This study highlights the utility of cross-neutralizing antibodies for designing bivalent or multivalent agents to provide a robust activity against circulating variants, as well as future SARS-like coronaviruses.
Effect of Human microRNA on Dengue Virus Genome: a cross-specie approach
Authors: Deb Anwesha 
Published: November 2022

AbstractDengue virus infection being a menace worldwide particularly in West Bengal demanded investigation of a new therapeutic approach. In our work we have performed in silico analysis of target specificity of miRNA to dengue virus genome. Several human miRNAs like hsa-miR-4758-3p_MIMAT0019904, hsa-miR-6760-3p_MIMAT0027421, hsa-miR-6839-3p_MIMAT0027581, hsa-miR-6842-3p_MIMAT0027587 are found to be directly interacting with dengue virus proteins like Envelope, NS1, NS3 and 3’UTR region. The target proteins of respective dengue proteins were analysed using stringDB and Cytoscape. Analysis of the target protein interaction network revealed ACTB, AKT1, TLR4 as few of the hub genes that can be indirectly regulated by miRNA and dengue genome interaction. Thus our work focuses on finding novel miRNA that are not known to regulate dengue infection till date and proposes a novel therapeutic strategy.