bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated molecular profiling of patient‐derived ovarian cancer models identifies clinically relevant signatures and tumor vulnerabilities
Authors: Lupia Michela , Melocchi Valentina , Bizzaro Francesca , Lo Riso Pietro , Dama Elisa , Baronio Micol , Ranghiero Alberto , Barberis Massimo , Bernard Loris , Bertalot Giovanni , Giavazzi Raffaella , Testa Giuseppe , Bianchi Fabrizio , Cavallaro Ugo 
Published: February 2022

AbstractHigh‐grade serous ovarian carcinoma (HGSOC) is a highly aggressive and intractable neoplasm, mainly because of its rapid dissemination into the abdominal cavity, a process that is favored by tumor‐associated peritoneal ascites. The precise molecular alterations involved in HGSOC onset and progression remain largely unknown due to the high biological and genetic heterogeneity of this tumor. We established a set of different tumor samples (termed the As11‐set) derived from a single HGSOC patient, consisting of peritoneal ascites, primary tumor cells, ovarian cancer stem cells (OCSC) and serially propagated tumor xenografts. The As11‐set was subjected to an integrated RNA‐seq and DNA‐seq analysis which unveiled molecular alterations that marked the different types of samples. Our profiling strategy yielded a panel of signatures relevant in HGSOC and in OCSC biology. When such signatures were used to interrogate the TCGA dataset from HGSOC patients, they exhibited prognostic and predictive power. The molecular alterations also identified potential vulnerabilities associated with OCSC, which were then tested functionally in stemness‐related assays. As a proof of concept, we defined PI3K signaling as a novel druggable target in OCSC.
Factors influencing the adoption of e‐government websites in Afghanistan from the citizens' perspective
Authors: Mohammadi Mustafa Kamel 
Published: February 2022

AbstractThe report by the United Nations in 2020 stated that Afghanistan was ranked 118th position in the e‐participation index which shows the low level of adoption among the Afghan citizens to online services offered by the e‐government websites in Afghanistan. As no previous research has been conducted to explore the influential factors on the adoption of e‐government websites in Afghanistan, this research fills the research gap by exploring the impact of website quality factors, trust factors, and technology adoption factors on intention to use e‐government websites from the citizens' perspective in Afghanistan. This research also investigates the impact of factors on the adoption of governmental websites at three levels (information, interaction, and transaction) of e‐government maturity. As part of the current research, the unified model of e‐government adoption (UMEGA) model was extended by integrating website quality factors and trust factors, which were extracted based on an extensive literature review. To validate the model, a survey of 294 Afghan citizens was conducted in four provinces of Afghanistan. The data were then analyzed using the partial least square‐structured equation modeling (PLS‐SEM) technique. The result revealed that websites quality, technology adoption, and trust factors have major impacts on the adoption of e‐government websites in Afghanistan, the impact of which varies depending on the level of e‐government maturity. Public administrators, officials, policymakers, website designers, and researchers have potential implications from the findings of this research regarding the adoption of governmental websites in Afghanistan.
Assessing feasibility of an adolescent relationship abuse prevention program for girls
Authors: Hill Ashley V. , Mistry Sejal , Paglisotti T.E. , Dwarakanath Namita , Lavage Daniel R. , Hill Amber L. , Iwuanyanwu Rosemary , Stokes Lynissa R. , Jones Kelley A. , Miller Elizabeth 
Published: February 2022

AbstractIntroductionSexual violence and relationship abuse are prevalent among adolescents and programs promoting gender equity, reproductive justice, and healthy relationships are key strategies for prevention. While such “gender transformative” approaches appear promising for boys, they have not been evaluated among girls. This study assessed the feasibility of this community‐based program, called Sisterhood 2.0, among girls in socially disadvantaged urban neighborhoods in Pittsburgh, Pennsylvania.MethodsThis quasi‐experimental trial examined feasibility of Sisterhood 2.0 (n = 246), delivered through 8 weekly sessions, assessed through attendance, retention and satisfaction. Participants completed surveys at baseline and end of program assessing other relevant measures. Generalized linear mixed models estimated changes from baseline to follow up comparing intervention to control participants.ResultsEleven neighborhoods were assigned to Sisterhood 2.0 (n = 5 neighborhoods) or job‐readiness training (n = 6 neighborhoods). Girls were between the ages of 13 and 19, 8–10th graders (59%), and self‐identified as Black (69%). Participants most often attended because they thought the program would be interesting (74%) and returned because of the women teaching the program (71%). Girls reported experiences with physical adolescent relationship abuse (ARA) (30% in both arms), emotional ARA (66% intervention; 56% control), or sexual ARA (11% intervention; 12% control). Physical ARA perpetration was high in both arms (intervention: 47%; control: 46%). Significant intervention effects were observed in recognition of abuse (β = 0.41, 95% confidence interval 0.03–0.78). No other significant intervention effects were observed.ConclusionsCommunity‐based gender‐transformative programming for girls is feasible and may be a promising approach for addressing interpersonal violence and promoting sexual health.
Therapeutic plasma exchange in a patient with acute motor axonal neuropathy subtype of Guillain‐Barre syndrome and systemic lupus erythematosus
Authors: Akgun Yamac , Langlie Jake , Huberman Melissa Ann , Wu Yanyun 
Published: February 2022

AbstractA young female in her early 20s with a history of systemic lupus erythematosus presented to the emergency department due to 4 days of progressive bilateral extremity weakness and numbness. The patient reported flu‐like symptoms that had spontaneously recovered 2 weeks prior to her presentation. She was 10 weeks pregnant at presentation. Lumbar puncture study and electrical muscle stimulation (EMS) were consistent with acute motor axonal neuropathy subtype of Guillain‐Barre syndrome (GBS). Patient also had increased proteinuria and renal biopsy performed that was consistent with lupus nephritis. Despite treatment with pulse dose corticosteroids and IVIG, the patient had minimal neurological improvement and with continued decline required intubation. Her pregnancy was terminated at this point and a course of therapeutic plasma exchange (TPE) was started. Patient was also treated with cyclophosphamide. The patient responded to the combination of therapy and had slow but gradual neurologic recovery as well as improvement of proteinuria. Here we describe a case of an acute motor axonal neuropathy (AMAN) subtype of GBS in a young woman with active SLE and current pregnancy at the time of the presentation. Concurrent GBS and active SLE in the setting of pregnancy may be more treatment resistant, and combination therapy including TPE, immunosuppression, and termination of pregnancy may be indicated.
Decomposable super‐simple BIBDs with block size 4 and index 4, 6
Authors: Chen Jingyuan , Yu Huangsheng , Abel R. Julian R. , Wu Dianhua 
Published: February 2022

AbstractDecomposable designs are important combinatorial designs, and have applications in constant weight codes, perfect threshold schemes and graph decompositions. Let be an even positive integer. A ‐decomposable super‐simple ‐balanced incomplete block design (BIBD) is a super‐simple ‐BIBD, , where , and each is a ‐BIBDs for . In this paper, for , it is proved that the necessary conditions for the existence of a ‐decomposable super‐simple ‐BIBD are also sufficient.
The Resistance Vignette Task: Validating a rapid measure of therapist skill at managing resistance
Authors: Di Bartolomeo Alyssa A. , Westra Henny A. , Javdan Salena , Olson David A. 
Published: February 2022

AbstractBackgroundTherapist appropriate responsivity to client ambivalence and resistance is considered an important interpersonal skill to avoid disengagement and ensure a continued collaborative, productive process. The present study examined the predictive validity of the newly developed Resistance Vignette Task (RVT), a 10‐item rapidly administered measure of therapist ability to appropriately respond to various presentations of client resistance.MethodsFollowing a resistance management workshop, the concurrent and prospective predictive capacity of RVT scores were examined through test interviews with ambivalent simulators and volunteers.ResultsProspectively, in test interviews with ambivalent interviewees, higher RVT scores immediately postworkshop were associated with significantly greater responsivity (appropriate responsivity and fewer responsivity errors) at 4‐month follow‐up. RVT scores at the 4‐month follow‐up point were also concurrently associated with significantly greater therapist responsivity and lower levels of interviewee resistance.ConclusionsThese findings provide further validation for the RVT as a measure of therapist responsivity in vivo, in actual interviews by predicting and being concurrently associated with therapist performance in response to client resistance. Thus, the RVT holds promise in advancing therapist training, as well as research on resistance as it represents an efficient measure of this key therapist skill.
Ego Impairment Index (EII‐2) as a predictor of outcome in short‐ and long‐term psychotherapy during a 5‐year follow‐up
Authors: Stenius Jaakko , Heinonen Erkki , Lindfors Olavi , Holma Juha , Knekt Paul 
Published: February 2022

AbstractObjectiveThis study examined the predictive ability of the Rorschach‐based Ego Impairment Index (EII‐2) on outcome of psychotherapy in different types and durations of therapy.MethodA total of 326 outpatients suffering from depressive or anxiety disorders were randomized into receiving solution‐focused (n = 97), short‐term psychodynamic (n = 101), or long‐term psychodynamic psychotherapy (n = 128). Psychotherapy outcome assessments during the 5‐year follow‐up period covered psychiatric symptoms, social functioning, and work ability.ResultsLower EII‐2 values, which indicate less problematic ego functioning, were found to predict faster improvement in both short‐term therapies as compared to long‐term psychotherapy.ConclusionThe results provide preliminary support for the utility of EII‐2 as a complementary measure to interview‐based methods for selecting between short‐ and long‐term therapies.
Synthesis of CuFe2O4‐Ti and CuFe2O4‐Ti‐GO nanocomposite photocatalysts using green‐synthesized CuFe2O4: determination of photocatalytic activity, bacteria inactivation and antibiotic degradation potentials under visible light
Authors: Özkal C.B. 
Published: February 2022

AbstractBACKGROUNDAdvanced oxidation processes (AOPs), specifically photocatalysis have gained an essential attraction in the field of micro pollutants degradation. Synthesis and use of proper photocatalysts for target pollutant and water matrix scenarios constitute the primary field of research.In this study, hydrothermal and sol‐gel synthesis methods were applied for the synthesis of nanocomposites in the form of spinel ferrite CuFe2O4 (CF) nanocomposites and CuFe2O4‐Ti (CFT) and CuFe2O4‐Ti‐GO (CFT‐GO) heterojunction nanocomposites with magnetic properties. And prior to photocatalytic activity based screening, bacteria inactivation and antibiotic degradation potentials of optimum NCs were determined under visible light conditions.PEG and Cydonia oblonga seed extract were used as chemical and bio‐chemical reducing‐stabilizing agents throughout the hydrothermal synthesis step. The as produced NCs were characterized by XRD, SEM, EDX, BET Surface, FTIR, RAMAN, Photoluminescence and Diffuse Reflectance Spectroscopy analysis.RESULTSAccording to the pre‐screening findings based on the methylene blue removal efficiencies of NCs under UV‐vis, CFT %1(w/w) have provided >70% removal at the end of 120 min. While CFT‐GO NCs yielded 30% removal rates (Pseudo first order kinetic constants of 0.012 and 0.0036 min−1 respectively). The reactive oxygen species formation by CFT‐GO NC photocatalysts was hindered as a result of conjugation mechanism between MB and aromatic regions of GO and due to high adsorption capacity of the NC.Based on photocatalytic bacteria inactivation findings of the pre‐screened NCs; >99% of bacteria were inactivated following 90 min of photocatalytic oxidation by CFT‐GO 3%. According to the Modified HOM model, k2 values were estimated to be 1.36 and 1.93 for CFT 1% and CFT‐GO 3% NCs, while no initial and post delay of bacteria inactivation was observed for the latter.Optimum photocatalyst dose was examined (to be 0.6 g L−1) based on photocatalytic degradation of sulfamethoxazole antibiotic at varying initial concentration ([C]0 SMX = 10 and 2.5 mg L−1). The CFT 1% photocatalysts have provided >89 % SMX degradation level (for [C]0 SMX = 2.5 g L−1). For the case of mixture of antibiotics [C]0 total = 2.5 g L−1, CFT 1% have provided >99.5%, >90%, >90% and 28% photocatalytic degradation levels at the end of 180 min for SMX AMP, TET and CLRT antibiotics respectively, while the total rate of degradation was 77%.CONCLUSIONBy using the green synthesis procedure, copper ferrite nanocomposites (CF) was successfully fabricated with comparable physical and chemical properties. This study not only reports on the green synthesis of common p‐type spinel CuFe2O4 but also offer new design of photocatalysts capable of providing efficient removal of antibiotics and bacteria inactivation under visible‐light irradiation. © 2022 Society of Chemical Industry (SCI).
COVID‐19 pathophysiology and ultrasound imaging: A multiorgan review
Authors: Ito Giovana N. W. , Rodrigues Vinícius A. C. , Hümmelgen Juliana , Meschino Gustavo S. P. G. , Abou‐Rejaile Gustavo M. , Brenny Isadora D. , de Castro Júnior Carlos R. , Artigas Rafaela C. , Munhoz João Pedro S. , Cardoso Gabriela C. , Picheth Guilherme F. 
Published: February 2022

AbstractCOVID‐19 is a dynamic disease and may affect different tissues and organs as it progresses. Therefore, the impact generated by the disease in all its stages and organs requires a functional and versatile imaging technique able to detect particularities or artifacts dynamically. Ultrasonography fulfills all these requirements and exhibit several advantages relative to other imaging modalities, including portability, lower cost and biosafety. Throughout the COVID‐19 pandemic, ultrasonography displayed a crucial role in the triage, monitoring, indicating organ damages and enabling individualized therapeutical decisions in COVID‐19 patients. This review is dedicated to highlight the main pathological effects correlated with ultrasound changes caused by COVID‐19 in the lungs, heart and liver.
ANKRD37 inhibits trophoblast migration and invasion by regulating the NF‐κB pathway in preeclampsia
Authors: Tan Wang , Fu Huijia , Zhou Xiaobo , Duan Yuhan , Yin Nanlin , Huang Jiayu , Liu Xiru 
Published: February 2022

AbstractBackgroundInadequate trophoblast invasion is associated with preeclampsia (PE). Ankyrin repeat domain protein 37 (ANKRD37) has been reported to be abnormally expressed in PE placentas. However, the role of ANKRD37 in trophoblasts has not been investigated. We aimed to determine the functions of ANKRD37 in PE and to explore the molecular mechanisms.MethodsHere, fluorescence in situ hybridization, immunohistochemistry, Western blotting and quantitative real‐time polymerase chain reaction were used to detect protein and mRNA expression levels. Cell counting kit‐8 assay, 5‐ethynyl‐2′‐deoxyuridine assay, flow cytometry, wound healing assay, transwell assay and RNA sequencing were performed to investigate the role of ANKRD37 and the underlying mechanism in HTR8/SVneo and JEG‐3 cells, and extravillous explant cultures were used to evaluate the migration and invasion abilities of extravillous cytotrophoblasts.ResultsWe found that ANKRD37 expression was upregulated in PE placentas compared to normal pregnancy placentas. ANKRD37 knockdown enhanced trophoblast migration and invasion, promoted extravillous explant outgrowth, and regulated the expression of key invasion proteins, whereas ANKRD37 overexpression exerted the opposite effects. RNA sequencing indicated that nuclear factor‐kappa B (NF‐κB) was the potential downstream pathway of ANKRD37, which was confirmed by the change in p‐p65 and p‐IκBα expression in JEG‐3 and HTR8/SVneo cells.ConclusionsOur findings suggest that high expression of ANKRD37 inhibits trophoblast cell migration and invasion possibly via the NF‐κB pathway, and may be related to the development of PE.
Unemployment in women with psychosocial disabilities during the COVID‐19 pandemic: Lessons from Tana River County, Kenya
Authors: Ebuenyi Ikenna D. , Gitonga Isaiah , Tele Albert , Syurina Elena V. 
Published: February 2022

AbstractIn low‐income settings, the informal economy is a practical alternative to work and employment for persons with disabilities. However, the COVID‐19 pandemic negatively affected the informal economy. This study aimed to explore the experiences of women with psychosocial disabilities in Kenya during the pandemic. We found that the pandemic worsened their experiences of work and employment, and they did not receive any social welfare or support from the government. Our findings suggest that pandemic management must adopt inclusive and context‐sensitive approaches that support persons with psychosocial disabilities. Social welfare and protection for persons with disabilities are relevant for socio‐economic empowerment and inclusion.
Kakonein restores hyperglycemia-induced macrophage digestion dysfunction through regulation of cathepsin B-dependent NLRP3 inflammasome activation
Authors: Lian Dawei , Zhu Li , Yu Yunhong , Zhang Xiaojuan , Lin Yike , Liu Jiaying , Han Ruifang , Guo Yitong , Cai Dongpeng , Xiao Wenjing , Chen Yulin , He Hong , Xu Danping , Zheng Chaoyang , Wang Xiao , Huang Yi , Chen Yang 
Published: February 2022

AbstractIn hyperglycemia-induced complications, macrophages play important roles in disease progression, and altered digestion is a key feature that dictates macrophage function. Recent evidence indicates that kakonein (Ka) possesses anti-inflammatory activities for hyperglycemia-induced complication. In this study, we established a mouse model of Nlrp3+/+ and Nlrp3−/− hyperglycemia and administering Ka, primary culture macrophages were tested by engulfing and digesting microbes. The role of macrophages in the cathepsin B–NLRP3 pathway involved in the mechanism of Ka in restoring macrophage digestion function was investigated using biochemical analyses, molecular biotechnology, and microbiology. Ka restored the function of macrophage digestion, which were same characterized by Nlrp3−/− mice. Meanwhile, kakonein could decrease NLRP3 inflammasome products expression and NLRP3/ASC or NLRP3/Casp1 colocalization in macrophage. Interestingly, Ka suppressed inflammasome response not by reducing NLRP3 and ASC expression but by reducing cathepsin B release and activation. And Ka restored macrophage digestion and inhibited NLRP3 inflammasome activation consistent with cathepsin B inhibitor. It is concluded that Ka reduced the release of lysosomal cathepsin B and consequently inhibited NLRP3 inflammasome activation to prevent macrophage digestion. Hence, Ka may contribute to new targets for treatment of hyperglycemia-associated dysfunction of macrophage digestion and development of innovative drugs.
Insights on the inhibition properties ofJatromollistatin(a cyclic heptapeptide) againstCrotalus adamanteusmetalloendopeptidase using molecular docking analysis
Authors: Jucá Thiago Lustosa , Ramos Márcio Viana , Cilli Eduardo Maffud , Neto Antônio Eufrásio Vieira , Mackessy Stephen P. , Monteiro‐Moreira Ana Cristina Oliveira 
Published: February 2022

AbstractJatropha mollissimais endemic to Brazil and is used for traditional medicinal purposes, including the treatment of snakebite. In this study, latex obtained from this plant was fractioned using reversed‐phase chromatography, and the fractions were then screened for peptides. A 755 g/mol peptide was obtained, and MS/MS analyses indicated it had a cyclic sequence (Pro‐Leu‐Gly‐Val‐Leu‐Leu‐Tyr). This peptide sequence was present in theJatrophagenome database, and an identity value of 90.71%, an E‐value of 0.0, and a score of 883 with NO‐associated protein 1/chloroplastic/mitochondria ofJatropha curcaswere obtained from the NCBI nonredundant protein sequence (nr) database. Molecular docking analyses performed with the peptide against a metalloendopeptidase belonging toCrotalus adamanteussnake venom suggested the cyclic peptide establishes favorable interactions with the catalytic site of the enzyme. Therefore, it could inhibit enzyme catalysis. This belief was corroborated by the formation of 6 hydrogen bonds with the linear form of the peptide. Tighter complexation of the cyclic form (41 kcal/mol more energetic) revealed better spatial blocking. The linear form outperformed the cyclic form in complexing the required energy, recruiting more catalytic residues (6/2), and in establishing more hydrogen bonds (6/3). However, cyclic folding provided a more significant spatial block within the catalytic site. The set of results suggests that the cycle peptide, here called Jatromollistatin, which was previously described as jatrophidin and pohlianin A in two other species of Jatropha, is a promising candidate to inhibit venom proteases. This belief is corroborated by the topical use of the latex for initial treatment of snakebites.
Analysis of influenza trend and impact of COVID‐19 in Kezhou, Xinjiang for 8 consecutive years
Authors: Zeng Lin , Zang Feng , Song Ninghong , Li Zhanjie 
Published: February 2022

AbstractTo study the trend of influenza and the impact of coronavirus disease 2019 (COVID‐19) in Kezhou, Xinjiang from 2013 to 2020. The data of influenza in Kezhou, Xinjiang from January 1, 2013, to December 31, 2020, were collected by the China Influenza Surveillance Information System to study the trend of ILI proportion, the distribution of influenza‐like cases in different age groups, the positive cases and positive rate of influenza, and the trend of different influenza subtypes, and to analyze the impact of COVID‐19 epidemic on influenza. The proportion of ILI in the Xinjiang Kezhou area was mainly children under 15 years old, and children under 5 years old accounted for the largest proportion. The proportion of ILI, the number of influenza‐positive cases, and the influenza‐positive proportion were mainly in winter and spring, especially in December and January each year. At the same time, this study found that the overall trend of H3N2 influenza in this region was on the rise, and the outbreaks in 2018 and 2019 were dominated by novel H1N1 and H3N2, respectively. The trend of influenza in Kezhou, Xinjiang is on the rise, and the prevention and control measures of COVID‐19 have significantly reduced the data of influenza. It is necessary to strengthen the vaccination work and maintain the basic prevention and control measures of respiratory infectious diseases to prevent and control influenza more effectively.