bluuebunny/crossref_metadata_2025_split huggingface dataset:
Studies on the Impact of Leaf Webber Diaphania pulverulentalis on Different Mulberry Varieties and Silkworm Bombyx mori L.
Authors: Bhagyamma C.T. , Kumari N. Vijaya 
Published: May 2022

Background: Leaf webber Diaphania pulverulentalis is identified as one of the major pest which infests mulberry gardens and cause damage to mulberry growth and development and reduced nutritive levels in leaves. As mulberry is the prime food source of silkworm Bombyx mori L. the leaf webber infested leaves fed to the silkworm shows greater impact on the yield of, cocoon both quantity and quality. Hence, the present investigation was carried out to understand the impact of leaf webber infestation on various popular mulberry varieties and on silkworm growth and cocoon yield. Methods: The study was conducted in selected ten mandals of Anantapuramu district, Andhra Pradesh during 2016-2019. The infested leaves were collected randomly form each mulberry garden and the same were analysed for various parameters like growth and development and biochemical parameters and silkworm bioassay. And analysed as per standard operating proceures. Result: Photosynthetic pigments of leaf webber infested mulberry leaves. i.e total chlorophylls in selected three mulberry varieties are decreased by an average of 40.17% and followed by carotenoids with a average decrease of 40.85%. Biomolecule components mainly proteins in selected varieties found to be decreased with the average percentage of 24.62 and carbohydrates and starch decreased with the average percentage of 47.09% 16.09% respectively. but in the phenols are increased with the average percentage of 57.39%, when compared with control (7.61%).
Propofol-diazepam or propofol-midazolam co-induction in healthy dogs: effects on propofol dosages, cardiovascular and respiratory events
Authors: Coward Joshua , Raillard Mathieu 
Published: May 2022

PICO question
In healthy dogs, does the use of diazepam or midazolam administered in co-induction with propofol result in a reduction in the dose of propofol required to induce anaesthesia and a decrease in adverse cardiovascular and respiratory events?

Clinical bottom line
Category of research question
Treatment
The number and type of study designs reviewed
Eight papers were critically reviewed. A total of six manuscripts were prospective, randomised, blinded, clinical studies. One trial was prospective, randomised, blinded, clinical with a Latin square, incomplete design. One study was retrospective, randomised, blinded, crossover, experimental
Strength of evidence
Moderate
Outcomes reported
Variables assessed in this Knowledge Summary included: propofol dose required to induce anaesthesia (considering successful orotracheal intubation as an end point), changes in cardiovascular variables (heart rate, systolic, mean and diastolic blood pressure) and changes in respiratory variables (development of apnoea, changes in respiratory rates)
Conclusion
In healthy dogs, using propofol-diazepam or propofol-midazolam co-induction resulted in a reduction in propofol dose required to induce anaesthesia in some trials only. Midazolam appeared more effective than diazepam in this context. The dosages, timing and sequence of drug administration seemed relevant. No evidence suggested that using propofol-diazepam or propofol-midazolam co-induction resulted in a reduction of adverse cardiovascular or respiratory events. In addition, although this was out of the scope of the PICO question addressed here, adverse events (e.g. excitement, poorer quality of induction) were reported in several studies when diazepam or midazolam were used in co-induction

How to apply this evidence in practice
The application of evidence into practice should take into account multiple factors, not limited to: individual clinical expertise, patient’s circumstances and owners’ values, country, location or clinic where you work, the individual case in front of you, the availability of therapies and resources.
Knowledge Summaries are a resource to help reinforce or inform decision making. They do not override the responsibility or judgement of the practitioner to do what is best for the animal in their care.
Yeast diversity in Cabernet-Sauvignon and Merlot grapes grown in the highlands of Southern Brazil
Authors: Mattos Rocha Ronaldo Kauê , Andrioli Joel , Scariot Fernando Joel , Schwarz Luisa Vivian , Longaray Delamare Ana Paula , Echeverrigaray Sergio 
Published: May 2022

Considering the influence of natural yeasts on wine production and the organoleptic properties of the final product, the objective of the present study was to evaluate the diversity of yeasts in Cabernet-Sauvignon and Merlot (Vitis vinifera L.) grown in the highlands of the Northeast region of Rio Grande do Sul State, “Serra Gaúcha”, Brazil. Grape samples were collected from commercial vineyards between February and March in the 2017, 2018 and 2019 vintages. One hundred sixty-six isolated yeasts were classified at the species level by D1/D2 domain of 26S rRNA sequencing. A total of 31 yeast species were identified. The most prevalent species were Hanseniaspora uvarum, Issatchenkia terricola, Saturnispora diversa and Starmerella bacillaris. These same yeasts were the most frequent regardless of the year evaluated. The results indicated that there is a great diversity of yeast species in grapes cultivated in the highlands of Southern Brazil. However, the yeast communities remain similar in Cabernet-Sauvignon and Merlot grapes the analysed parameters (grape variety/vintage) are not interfering in the yeast populations found in the region highlands of Rio Grande do Sul, “Serra Gaúcha”, Brazil. This study enabled the knowledge of the yeast populations present in the region and their variations during the harvesting of wine grapes, showing that there is a majority pattern of species independent of the harvest.
Occurrence, biogenesis and sensory impact of methyl salicylate in Lugana wines
Authors: Slaghenaufi Davide , Luzzini Giovanni , Avrini Giulia , Marconcini Stefano , Vela Eduardo , Ugliano Maurizio 
Published: May 2022

Lugana wines are produced in the winemaking regions of Veneto and Lombardia, employing Turbiana grapes grown in the proximity of the Garda Lake. Methyl salicylate (MeSA) has been reported as a potentially impactful compound in Lugana wines. The aim of this study was to evaluate the occurrence of MeSA in commercial Lugana wines, elucidate its formation during winemaking and aging, and assess its potential contribution to Lugana aroma. MeSA was quantified in a total of 93 samples including Lugana, Verdicchio (another Italian white wine produced in central Italy using Turbiana grapes locally referred to as Verdicchio), as well as other Italian white and red wines such as Corvina, Corvinone, Garganega, Sangiovese, Pinot nero, Pinot grigio. The results showed that Lugana showed an average concentration of MeSA of 50.6 µg/L, Verdicchio of 33.4 µg/L, while the rest of the wines showed concentration below 5 µg/L. These results indicate that MeSA can be considered a varietal marker of Lugana wines, as well as more in general of wines obtained from the Turbiana/Verdicchio varietal cluster. Concentration of free MeSA in Lugana grape must is however low, and experimental fermentations showed that MeSA was largely formed by yeast activity during alcoholic fermentation, which hydrolyzes the glycosidic precursors of MeSA present in the must to release the aglycone. Additional MeSA can be formed in wine during aging by acid hydrolysis of the glycosidic precursors. Finally, the olfactory threshold of MeSA in a commercial Lugana was estimated by BET method at 38 µg/L, suggesting that MeSA could play a role in Lugana aroma.
NOTECNOLOGIAS VERDES PARA TRATAMENTO DE CÂNCER - UMA REVISÃO
Authors: Sangalli Thiago Leonardo 
Published: May 2022

As nanopartículas (NPs) verdes são substratos naturais extraídos de recursos biológicos disponíveis na natureza. O objetivo do presente estudo foi revisar a atividade anticâncer de NPs sintetizadas a partir de extratos verdes contra células tumorais. Selecionou-se 21 artigos no banco de dados eletrônicas, PubMed e Cochrane, trabalhos publicados nos últimos 5 anos, em inglês, português e espanhol. Os resultados mostraram o potencial anticâncer das nanopartículas biogênicos contra diferentes células cancerígenas, cerca de 95% dos estudos mostram algum feito citotóxico no tumor. A linhagem das células tumorais mais estudada foi a de origem hepática, 80% dos artigos avaliaram o resultado das NPs no carcinoma hepatocelular (HepG2), e obtiveram resultados surpreendentes, a segunda linhagem de células mais estuda foi adenocarcinoma de mama (MCF-7). A citotoxicidade nas células tumorais foi avaliada através da concentração inibitória mínima (I50), e em nível molecular observou-se a presença de células apoptóticas, bem como o aumento de espécies reativas de oxigênio (ROS) intracelular e de expressões das proteínas caspases e a diminuição de enzimas anti apoptóticas. Pode-se concluir que as NPs verdes são uma alternativa eficaz no tratamento de câncer, no entanto estudos devem avaliar a atividade anticancerígena por meio de estudos in vivo em humanos.
Special Issue on Smart Factory
Authors: Hibino Hironori , Nishioka Yasuyuki 
Published: May 2022

A Fourth Industrial Revolution has been proposed, and various research and development activities geared toward the realization of smart factories have become extremely active. In smart factories developed individually within companies, the direction of the development activities is changing drastically. Regarding common technologies, research activities and feasible case study activities that are collaborations among industry, academia, and government and that show consideration and awareness of ecosystems are becoming active.
It has been five years since the IJAT last published a special issue on smart manufacturing. Smart factory technologies are becoming more and more important in the industrial world, not as a temporary boom, but as a steady development. Research on smart factory technologies is progressing, as they are considered to be important technologies in the industrial world, along with Digital Transformation (DX) technologies, among others.
This special issue addresses the latest in advanced research on smart factories. We have received many submissions from researchers at universities, public research institutes, and companies, and we are pleased to publish eight papers. The latest research being published mainly concerns the following five topics:
- Smart and digital twinning of machine tools
- Smart and digital twinning of production systems
- Optimization of production systems using scheduling algorithms
- Optimization of production systems including worker operations
- Optimization of energy consumption for production systems
The results of these studies will serve as a reference for further smart factory technologies in the future.
We deeply appreciate the careful work of all the authors, and we thank the reviewers for their incisive efforts. Without these contributions, this special issue could not have been created. We also hope that this special issue will trigger leading to further advances in smart factories.
Association Between Breakfast Consumption and Anthropometrically Determined Nutritional Status of Secondary-School Adolescents in Lagos, Southwest Nigeria
Authors: Olatona Foluke Adenike , Oloruntola Oyetola , Adeniyi Oluwafunmilayo , Amu Eyitope 
Published: May 2022

Background and Objectives: Breakfast skipping has been associated with obesity among adolescents in some studies but little is known about the relationship between breakfast consumption and obesity among secondary-school adolescents in Nigeria. This study contributes to the empirical literature by analyzing the relationship between breakfast consumption and anthropometrically determined nutritional status of secondary-school adolescents in Lagos, Nigeria.
Methods: This was a descriptive cross-sectional study in which multi-stage sampling was used to select 397 secondary-school adolescents (10 to 19 years, mean = 13.8 ± 1.7 years).They were classified into student groups from public or private schools in Lagos. Data were collected using a semi-structured self-administered questionnaire and analyzed using Epi-info version 7. The independent variables were the proportion of adolescents who had a high level of knowledge about breakfast consumption, while the outcome variable was the proportion of adolescents who were overweight or obese. World Health Organization (WHO) AnthroPlus software was used to determine the nutritional status of adolescents. Mean and standard deviations were computed for continuous variables, and frequency tables were generated for categorical variables. Significant associations between variables were obtained using Chi-square with the level of significance set at p < 0.05.
Results: Only 17% of the adolescents had good knowledge of breakfast consumption. More than half (57.4%) of the participants ate a daily breakfast. The percentage of adolescents who skipped breakfast was higher among older 16-19 years (52.2%) and middle 13-15 years (43%) than the younger adolescents (34.7%). Girls skipped breakfast more than boys. Most respondents were in the normal Body Mass Index (BMI) ranges for their genders. Prevalence of overweight and obesity were 7.1% and 3.3% among males and 7.1% and 2.8% among females respectively. The mean BMI of those who skipped breakfast (19.33 ± 3.27kg/m2) was significantly higher than the BMI of those who ate breakfast (18.56 ± 3.05 kg/m2) (p=0.019). Most of the adolescents who ate a daily breakfast had mothers who had completed only primary school education although the relationship was not statistically significant (χ2=5, p=0.172).
Conclusion and Global Health Implications: Breakfast knowledge was low, while breakfast consumption was average. Adolescents who skipped breakfast had a significantly higher BMI (19.33± 3.27kg/m2) than those who ate breakfast (18.56 ± 3.05 kg/m2) (p=0.019). Nutrition education that emphasizes the importance of breakfast consumption with the purpose of behavioral change should be intensified among adolescents.

Copyright © 2022 Olatona et al. Published by Global Health and Education Projects, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution License CC BY 4.0.
Diseño y validación de contenido de un instrumento de evaluación de la competencia acuática preventiva en jóvenes de 11 y 12 años
Authors: Ortiz Olivar Ana , Moreno Murcia Juan Antonio 
Published: May 2022

Antecedentes: El ahogamiento se ubica entre las primeras causas de muertes por lesiones no intencionales en niños de 10 a 14 años a nivel mundial. La Organización Mundial de la Salud (2014) plantea que esta lesión es totalmente prevenible desde intervenciones que promuevan múltiples capas de protección (OMS, 2014).
Objetivos: Diseñar y dar validez de contenido a un instrumento que permita evaluar las competencias acuáticas preventivas de flotación, propulsión, de orientación asociadas a la autoevaluación y la toma de decisiones en diferentes situaciones en jóvenes de 11 y 12 años.
Método: La validación de contenido se efectuó mediante el acuerdo y consenso de 9 expertos a través de la técnica Delphi. Tras la recogida de las aportaciones por medio del juicio de expertos, se ajustó la escala acorde a las indicaciones y se procedió a una segunda revisión para obtener un coeficiente de Kendall adecuado. La validez de comprensión fue comprobada tras la aplicación de la escala a una muestra independiente de jóvenes.
Resultados: Se presenta la Escala de Evaluación de la Competencia Acuática Preventiva de 11 a 12 años con una adecuada validez de contenido que evalúa el estado actual de la competencia motriz acuática preventiva, en relación a las competencias subacuáticas, competencias de flotación, competencias de nado y competencias de nado con orientación.
Conclusiones: El instrumento de Evaluación de la Competencia Acuática preventiva permite diferentes expresiones de las competencias acuáticas preventivas brindando un recurso importante para su aplicación en diversos contextos.
Palabras clave: educación acuática, ahogamiento, autoevaluación, prevención, natación, medición.
Localization of small peripheral pulmonary nodules for surgical resection: a new intraoperative technique in hybrid operating room
Authors: Yu Hanbo , Tian Wenxin , Sun Yaoguang , Wu Qingjun , Ma Chao , Jiao Peng , Huang Chuan , Li Donghang , Tong Hongfeng 
Published: May 2022

Abstract
Objective The purpose of this study was to introduce a new feasible and effective intraoperative localization technique for small peripheral pulmonary nodules in hybrid operating room.Methods Between June 2020 and June 2021, the intraoperative localization was performed in 27 patients for 35 small pulmonary nodules at our institution. The procedure was undergone under thoracoscopic observation. After making the VATS ports, a titanium clip was clipped at the visceral pleura as near the pulmonary nodule as possible to be a marker for the nodule. VATS resection was performed next.Results A total of 27 patients were included in this study, including 6 males and 21 females. The median age was 58 years (range 34-78 years). All surgeries were performed by two-port VATS. A total of 35 pulmonary nodules underwent intraoperative localization. The mean diameter of nodules was 10.6 ± 3.7 mm. The distance of nodules to visceral pleura was 8.3 ± 8.7 mm. The mean localization time was 23.3 ± 3.3 min. The median time of C-arm scanning was 3 (range, 2-4) times. All the nodules were localized successfully and resected precisely. No VATS were converted to thoracotomy. There were no complications related to localization procedures.Conclusions This new intraoperative localization technique was feasible, safe and effective. And also the intraoperative procedure could avoid extra suffering for patients.
Rapid anticalcification treatment for glutaraldehyde-fixed autologous tissue in cardiovascular surgery
Authors: Kaneko Shotaro , Isoda Susumu , Aoyama Toru , Goda Motohiko , Yasuda Shota , Shibuya Taisuke , Matsumura Mai , Mitsui Hideaki , Okudela Koji , Suzuki Shinichi , Machida Daisuke , Masuda Munetaka 
Published: May 2022

Abstract
Background
Glutaraldehyde (GA)-fixed autologous tissues, including the pericardium, are widely used as patches and valve substitutes in cardiovascular surgery. However, the treatment causes tissue calcification. No rapid anticalcification treatment method has been established for use during surgery. Here, we aimed to establish a rapid method for anticalcification treatment.
Methods
Thoracic aorta tissues were first fixed with GA for 3 min, and post-treated with ethanol for 0, 10, 20, and 30 min representing groups 2, 3, 4, and 5, respectively; untreated tissues served as the control (group 1). The treated tissues were subdermally implanted into 3-week-old male Wistar rats for 28 days. The calcification level in each explant was evaluated semiquantitatively by annotating and measuring the area using virtual slides; the data were statistically analyzed.
Results
Semiquantitative calcification analysis revealed that the calcification of implants from the untreated group (group 1; P = 0.0014) and groups 4 (P = 0.0014) and 5 (P = 0.0031) was significantly lower than that from group 2. Meanwhile, implants from group 3 showed a tendency towards decreased calcification, although this was not significant (P = 0.0503).
Conclusions
A rapid ethanol treatment might prevent the calcification of GA-fixed tissues in a rat model of subdermal implantation. This method may facilitate an effective and rapid anticalcification treatment of autologous tissues for use during cardiovascular surgery.
Association between Duddingtonia flagrans, Dimethylsulfoxide and Ivermectin for the control of Rhabditis spp. in cattle
Authors: Sobral Samilla Alves , Ferraz Carolina Magri , Souza Rômulo Inácio Lopes , Queiroz Luanderson Mendes , Reinó Natália , Junior Otavio Luiz Fidelis , Tobias Fernando Luiz , Correia José Antônio Lima , de Araújo Jackson Victor , Vilela Vinícius Longo Ribeiro , Soares Filippe Elias Freitas , Braga Fabio Ribeiro 
Published: May 2022

Abstract
Cattle parasitic otitis caused by the nematode Rhabditis spp. is a serious health problem in Brazil, since it does not have an effective control. In vitro studies associating biological control and chemical control as an alternative method showed promising results. The objective of the present study was to evaluate the combined use of the fungus Duddingtonia flagrans (AC001), 10% dimethylsulfoxide and 1.87% Ivermectin for the in vivo control of Rhabditis spp., in naturally infected Gyr cattle. For this purpose, 48 animals, whose infection in both ears was diagnosed, were randomly assigned to 6 groups: Group 1 (Ivermectin 1.87%); Group 2 (10% dimethylsulfoxide); Group 3 (AC001); Group 4 (Ivermectin 1.87% + dimethylsulfoxide 10%); Group 5 (1.87% Ivermectin + AC001); Group 6 (10% dimethylsulfoxide + AC001). The treatments were performed in a single dose, in the right ears, with the left ears remaining untreated, as a control group. There was a significant reduction (p&lt;0.01) in the recovery of nematodes in the treated groups in relation to the control, with the following best efficacies: Groups 1 and 2, 47% and 52.9%, respectively, seven days after treatment; Groups 3, 4 and 5, 47.8%, 48.6% and 36.7%, respectively, 14 days post-treatment; Group 6, 38.4%, 21 days post-treatment. It was concluded that the combination of chemical compounds and D. flagrans in a single application were effective for the in vivo control of Rhabditis spp. in naturally infected cattle.
Optimization of antimicrobial conditions and isolation of antimicrobial substances from Bacillus amyloliquefaciens X8
Authors: wang weixin , Liu Youhua , Xing Shaopeng , Zhao Mingguang , Wang Rui , Xu Weikang , Yang Qiaoqiao , Li Liantai 
Published: May 2022

Abstract
Purpose
This study sought to employ the response surface methodology (RSM) to investigate factors that affect Bacillus amyloliquefaciens X8 bacteriostasis.
Methods
Design Expert 8.0.6 was used to perform multiple regression fitting and create antimicrobial mathematical models to optimize the antimicrobial effect of Bacillus amyloliquefaciens X8. A Shodex Protein KW-2004 gel column and a C18 column coupled with a high-performance liquid chromatography (HPLC) system were used to separate and purify the antimicrobial substances extracted from Bacillus amyloliquefaciens X8 (X8-AS). An Agilent 6410 Triple Quad LC/MS system and Nicolet-is10 system were used to analyze X8-AS via liquid chromatography mass spectrometry (LC-MS) and infrared spectroscopy (IR).
Results
The results demonstrated that the optimum antimicrobial conditions for Bacillus amyloliquefaciens X8 (at which antimicrobial activity was increased by 22.3%) were pH 6, 30 ℃, and 36 h of incubation.
Conclusion
The results showed that the relative molecular weight of X8-AS was 1413.5 Da. After searching through the infrared spectrum material library, X8-AS was found to be highly similar to chondroitin sulfate.