bluuebunny/crossref_metadata_2025_split huggingface dataset:
TOP2A and PPARGC1A differentiate chromatin regulator-related subtypes for prostate cancer patients undergoing radical prostatectomy
Authors: Shi Xu , Zhu Weizhen , Feng Dechao , Li Dengxiong , Han Ping , Wei Wuran 
Published: November 2022

Abstract
BACKGROUND
To find molecular subtypes and risk score that can be used to accurately predict the biochemical recurrence (BCR) after radical prostatectomy (RAP) in prostate cancer (PCa) patients from the perspective of chromatin regulator (CR).
METHODS
We obtain the differentially expressed genes (DEGs) between tumor and normal samples from the TCGA and GEO databases, and intersected it with CR-related and prognostic genes in the TCGA database. Subsequently, consensus clustering, risk score, functional analysis, tumor immune microenvironment, m6A analysis, and tumor heterogeneity were analyzed through R software 3.6.3 and its suitable packages.
RESULTS
After taking the intersection, TOP2A and PPARGC1A were identified to construct molecular subtypes and risk score in the TCGA database, which was verified externally using another GEO dataset. Cluster 2 had shorter BCR-free survival than cluster 1 in TCGA (HR: 2.21, 95%CI: 1.32–3.73, p = 0.003), GEO (HR: 2.05, 95%CI: 1.05–4.02, p = 0.01) and MSKCC2010 (HR: 5.93, 95% CI: 1.96–17.87, p < 0.001) databases. Similar results were observed in the high- and low-risk group divided by our risk score on the basis of median value. We have witnessed more significant tumor heterogeneity and higher expression of various m6A genes in the cluster 2. Gene set variation analysis indicated that cell cycle-related pathways were mainly downregulated in the cluster 2. In addition, despite no overall differences in tumor immune microenvironment scores, various tumor-infiltrating immune cells were suppressed in the cluster 2.
CONCLUSIONS
In our study, TOP2A and PPARGC1A could differentiate prostate cancer subtypes for patients undergoing RAP and help make early plans for subsequent treatment.
Diversity of fungal communities and changes in antibacterial activity of artificially culturable fungal secondary metabolites from diseased and healthy fruits of Cinnamomum burmannii
Authors: Wang Wei , Cai Teng , Yang Yuting , Guo Hui , Shang Zhuo , Shahid Hamza , Zhang Yirong , Qiu Sirun , Zeng Xinnian , Xu Xiaoli , Liu Yi , Fang Ping , Ding Ping , Mao Ziling , Shan Tijiang 
Published: November 2022

Abstract
Background
Studies on the function of endophytic fungal communities in healthy plant tissues are not new, while little is known about the functional changes of fungal communities in pathogenic plant tissues. Healthy fruits of Cinnamomum burmannii are an important medicinal resource, infection by phytopathogenic fungi causes changes in volatile components, with the corresponding, the functional characteristics and potential value of fungal communities in diseased fruits have not been reported. Consequently, characterization in the composition structure and antibacterial activity of fungal communities from healthy and diseased fruits of C. burmannii was investigated, the secondary metabolites in the fungus Medicopsis romeroi were reported for the first time.
Results
The fungal community in diseased fruits differed from that of healthy fruits at the Phylum, Class, Order or Genus levels, with important changes in the species and relative abundance of the dominant flora. Forty-one different strains (11 from healthy fruits and 30 from diseased fruits) were successfully identified by morphological and molecular biological methods which were classified into 8 groups and 23 genera using phylogenetic tree analysis, with Pleosporales, Glomerellales and Hypocreales were the dominant group at the phylum level and Colletotrichum was the dominant group at the genus level. The secondary metabolites of all strains had different degrees of antibacterial activity, while the secondary metabolites of diseased fruit symbiotic fungi were generally stronger than those of healthy fruits, with the active secondary metabolites dominated by small and medium polar compounds. Both the up-regulated and down-regulated flora in diseased fruit had strong antibacterial activity. Two new compounds, 5, 6-Dimethoxy-[1',1:4,1''-terphenyl]-2-ol (1), 5-(methoxycarbonyl)-2-methylbenzo[d][1, 3]dioxole-2-carboxylic acid (2) and three known compounds (3, 4, 5) were isolated and identified for the first time from the symbiotic fungus Medicopsis romeroi.
Conclusion
Although the diversity of fungal communities decreases after plant fruit disease, the antibacterial activity capacity of the fungi among them is generally enhanced, and the development of secondary metabolites of active strains from diseased fruits holds great promise. This study is significant for understanding the functional variation of bioactivity in fungal communities and developing a broader range of bioactive resources.
The Device Simulation of MXene-added Hole-Transport Free Perovskite Solar Cells
Authors: Azadi Saeid Khesali , Asgharizadeh Saeid 
Published: November 2022

Abstract

Perovskite solar cells (PSCs) without hole transport layer (HTL) based on organic and inorganic metal halide perovskite have received vast consideration in recent years. For predigestion of device structure and construction process, the exclusion of the HTL is a marvelous way. By detaching the HTL part of the devices, we could reduce the cost and complexity of the structures. Currently, a novel 2D material named Ti3C2 MXene with high electron mobility, excellent metallic conductivity, and functionalized surface groups applied for tuning the energy offsets has been reported to be added in the perovskite absorber layer, leading to a remarkable power conversion efficiency (PCE) improvement. In this work, the SCAPS-1D simulator is employed to optimize the response of the MXene-assisted HTL-free photovoltaic device to obtain the highest possible efficiency and investigate the physics behind such simulation. We present a modeling procedure that optimizes the length of the involved layers and specifies the optimum level of the doping concentration. We also show that by optimizing the work function of the back contact, the device performance witnesses a significant improvement, proving the considerable role of the back contact in these cells. The simulated HTL-free devices illustrate attainably PCEs of about 20.01% and 21.04% for the cells without and with MXene, under AM 1.5G illumination and absorption up to 760 (nm).
Exposure to oxLDL impairs TGF-β activity in human tendon cells
Authors: Mousavizadeh Rouhollah , Waugh Charlie M. , DeBruin Erin , McCormack Robert G. , Duronio Vincent , Scott Alex 
Published: November 2022

Abstract
Background: Hypercholesterolemia is a known risk factor for the development of acute or chronic tendon injuries, but the mechanisms which link cholesterol and tendon pathology are not completely known. Previous studies have shown that patients with hypercholesterolemia experience elevated levels of oxidized LDL (oxLDL), a molecule which triggers inflammation and collagenase activity. In this study we discovered a potential mechanism that may contribute to tendon damage by oxLDL.
Methods: Isolated human tendon cells were incubated in the presence or absence of oxLDL. The influence of oxLDL on the expression level of key mRNA and proteins was examined using real time quantitative PCR, ELISA and Western blots. The activities of enzymes relevant to collagen synthesis and breakdown (lysyl oxidase and matrix metalloproteinases) were quantified using fluorometry. Finally, the isolated human tendon cells in a 3D construct were exposed to combinations of oxLDL and TGF-β to examine their interacting effects on collagen matrix remodeling.
Results: The expression of key mRNAs including TGFB, COL1A1, DCN, and LOX were reduced in human tendon cells by oxLDL while MMPs were increased. The oxLDL reduced the activity of LOX, whereas conversely MMP activities were induced. COL1A1 synthesis and TGF-β secretion were also inhibited. Adding recombinant TGF-β reversed the effects of oxLDL on the expression of collagens and LOX. OxLDL also impaired collagen matrix remodeling, and adding TGF-β restored the native phenotype.
Conclusions: Exposure oxLDL in patients with hypercholesterolemia may adversely affect the mechanical and structural properties of tendon tissue through a direct action of oxLDL on tendon cells, including impairment of TGF-β expression. This impairment leads to disturbed matrix remodeling and synthesis, thereby potentially leading to increased risk of acute or chronic tendon injury.
Assessment of Outdoor Thermal Comfort using Landsat 8 Imageries with Machine learning tools over a Metropolitan City of India
Authors: Prasad P. S. Hari , Satyanarayana A. N. V. 
Published: November 2022

Abstract
Rapid urbanization and an enormous increase in the urban landscape have resulted in a drastic change in land surface temperature (LST) and outdoor thermal comfort in metropolitan cities and severely impact the health and well-being of residents. The thermal comfort of any region depends on various parameters such as atmospheric temperature, relative humidity, land use, and land cover classes (vegetation, water, built-up and barren). In the present study, an attempt has been made to understand the spatial variation of outdoor thermal comfort in a metropolitan city, Hyderabad (17° 23’ 13” N, 78° 29’ 30” E), India. High-resolution satellite imageries of Landsat 8 and available in situ meteorological observations are used for this purpose. Various environmental indices such as NDVI (Normalized difference vegetation index), NDWI (normalized difference water index), NBI (new built-up index), LST, brightness, greenness, and wetness are estimated using remote sensing techniques. The machine learning tool (SVM regression) was implemented to model the outdoor thermal comfort at a finer resolution. The spatial variation of outdoor thermal comfort was studied for the summer and winter seasons of 2018, 2019, and 2020 and analyzed to delineate the comfort and discomfort zones over the city. The results suggest that urban built-up and barren lands cause maximum discomfort to pedestrians, and vegetated areas and water bodies of urban spaces substantially decrease the thermal loads. Significant spatial variation of outdoor thermal conditions is noticed over different regions of the city, portraying the influence of the urban landscape.
Green extraction of phenolics and flavonoids from black mulberry fruit using natural deep eutectic solvents: optimization, kinetics and surface morphology
Authors: Vo Tan Phat , Pham Thuy Vy , Weina Kasia , Tran Thi Ngoc Huyen , Vo Le Thao Vy , Nguyen Phuc Thanh , Bui Tran Linh Ha , Phan Thuy Han , Nguyen Dinh Quan 
Published: November 2022

Abstract
This study deployed ultrasonic-assisted extraction (UAE), combined with natural deep eutectic solvents (NADES), to extract phenolics and flavonoids from the black mulberry fruit, and the antioxidant activity was examined. The extraction yields of NADES-based UAE were assessed based on total phenolic and flavonoid contents (TPC and TFC, respectively). NADES produced from choline chloride-lactic acid at a 1:2 molar ratio had the highest extraction yield. One-factor experiments evaluated the effect of UAE conditions (liquid-to-solid ratio, water content in NADES, temperature, and time) on TPC, TFC, and antioxidant activity. Response surface methodology with the Box-Behnken design model optimized the NADES-based UAE process based on response (TPC, TFC, ABTS, OH, and DPPH). The optimal conditions for the NADES-based UAE process were 70 ml/g of LSR, 38.9% water content in NADES, 67.9°C, and 24.2 min of retention time. The predicted values of the Box-Behnken design were compatible with the experimental results. Moreover, scanning electron microscopy was used to survey the surface of black mulberry fruit with and without sonication. This study proposes an efficient and green method for extracting bioactive compounds from the black mulberry fruit.
Blockade of CCR5 and CXCR3 attenuates murine acute graft-versus-host disease through modulating donor-derived T cell distribution and function
Authors: Tang Bo , Qin Chenchen , Liu Huihui , Miao Shengchao , Wang Zhenhua , Zhang Yang , Dong Yujun , Liu Wei , Ren Hanyun 
Published: November 2022

Abstract

Background
Lymphocyte trafficking via chemokine receptors such as CCR5 and CXCR3 plays a critical role in the pathogenesis of aGVHD. Our previous studies showed that addition of CCR5 or CXCR3 antagonist could only slightly alleviate the development of aGVHD. Given the specificity of T lymphocytes bearing CXCR3 and CCR5, we investigated whether combined CCR5 and CXCR3 blockade could further attenuate murine aGVHD.
Methods
A mouse model of aGVHD was established to assess the efficacy of CCR5 or/and CXCR3 blockade on the development of aGVHD. The distribution of lymphocytes was calculated by quantification of immunostaining cells. The immunomodulatory effect on T cells were assessed by evaluating T- cell proliferation, viability, and differentiation.
Results
Using murine allo-HSCT model, we demonstrated that blockade of both CCR5 and CXCR3 could efficiently alleviate the development of aGVHD. Further investigation on the immune mechanisms for this prophylactic effect showed that more T cells were detained into secondary lymphoid organs (SLOs), which may lead to reduced infiltration of T cells into GVHD target organs. Our study also showed that T cells detained into SLOs dampened the activation, suppressed the polarization toward Th1 and Tc1, and induced the production of Treg cells.
Conclusion
These data suggest that concurrent blockade of CCR5 and CXCR3 attenuates murine aGVHD through modulating donor-derived T cell distribution and function, and this might be applicable for aGVHD prophylaxis in clinical settings.
Heartworm, Dirofilaria immitis, in carnivores kept in zoos located in Texas, USA: Risk perception, practices, and prevalence
Authors: Upton Kaitlyn E. , Budke Christine M. , Verocai Guilherme G. 
Published: November 2022

AbstractBackgroundDirofilaria immitis, the causative agent of heartworm disease has long been established to affect both wild and domestic canids, felids, and mustelids. Recent literature demonstrates that additional families in the order Carnivora are also susceptible. The objectives of this study were to 1) better understand current practices surrounding heartworm prevention and diagnostics in zoological facilities located in the state of Texas, USA and 2) assess the seroprevalence ofD. immitisinfection among terrestrial carnivores kept in these facilities. Methods A questionnaire was completed by veterinarians or veterinary technicians representing 10 zoological facilities across Texas. This questionnaire was designed at the taxonomic family level, encompassing the 12 terrestrial carnivore families: Ailuridae, Canidae, Eupleridae, Felidae, Herpestidae, Hyaenidae, Mephitidae, Mustelidae, Prionodontidae, Procyonidae, Ursidae, and Viverridae. The second objective was achieved with the use of archival serum samples made available by six zoo facilities. Results Risk perception varied across facilities for every family, including among species belonging to Canidae. All facilities used monthly heartworm prevention in canids and felids, with more variation existing in the other families. The use of diagnostic testing and type and route of administration of preventive varied by facility, with oral ivermectin the most commonly used preventive. A total of 217 archival serum samples, belonging to 211 individual animals encompassing 11 families and 39 species, were tested with a commercial heartworm antigen ELISA test, pre- and post-immune-complex dissociation. A subset of samples was also assessed for presence of feline anti-heartworm antibodies using a commercial ELISA test. Two animals had antigen detected (0.95%), both Asian small-clawed otters from the same facility. Conclusions This study demonstrates that while the zoo veterinary community is aware of the risk and health impact of heartworm disease in canids and felids, there is still a great deal of uncertainty regarding the risks and ideal strategies for prevention in other carnivore families. The low prevalence found in zoo carnivores in Texas may serve as a baseline for future prevalence studies across the southern United States amid the emerging concern of macrocyclic lactone resistance.
Elevated creatinine kinase levels amongst Dutch adolescents with acute alcohol intoxication
Authors: Pigeaud Louise , de Veld Loes , Lely Nico van der 
Published: November 2022

Abstract
Purpose: This study aims to explore the prevalence of creatinine kinase elevation amongst a sample of Dutch adolescents admitted for acute alcohol intoxication.
Methods: The data on all admitted adolescents <18 years old with acute alcohol intoxication between 2008 and 2021 were collected from a Dutch major district general hospital, Reinier de Graaf Gasthuis, in Delft. Overall, 495 adolescents who were treated for symptoms of acute alcohol intoxication during this period were included in the study.
Results: When evaluating the blood samples of the included patients, elevated creatinine kinase levels were found in 60% of the cases, with a mean of 253.83 U/I. A confirmed diagnosis of rhabdomyolysis (increase in CK > 5-fold the upper limit of normal) was present in 4.4% of cases. Moreover, using a linear regression this study found that a higher blood alcohol concentration was associated with higher creatinine kinase levels, when adjusted for positive drug screenings amongst the adolescents with acute alcohol intoxication (p = 0.027; β = 66.88; 95% CI 7.68-126.08).
Conclusions: This is the first study focusing on how acute alcohol intoxication affects adolescents’ muscle tissue. The results could potentially help to prevent alcohol use within the sports world. It could also aid understanding of how acute alcohol intoxication influences the breakdown of adolescents’ muscle tissue.
Dietary inclusion of Calopogonium mucunoides, Ocimum gratissimum, and Tridax procumbens improves growth performance, haemato-biochemical profile and disease resistance in Nile tilapia, Oreochromis niloticus
Authors: Kusi Isaac Osei , Obirikorang Kwasi Adu , Adjei-Boateng Daniel 
Published: November 2022

Abstract
The study evaluated the effect of Calopogonium mucunoides (CM), Ocimum gratissimum (OG) and Tridax procumbens (TP) supplemented diets on growth, blood health and disease resistance in Nile tilapia, Oreochromis niloticus. Fish (40.1 ± 0.7g initial weight; n = 15 per tank) in triplicate treatments were fed the supplemented diets at 5% body weight daily for 70 days. Growth performance, feed utilization, haemato-biochemical parameters and resistance against Streptococcus agalactiae were determined after the feeding trial. The results showed that weight gain (g) and specific growth rate (% day–1) of TP (62.6 ± 2.2; 1.4 ± 0.04), CM (61.3 ± 1.7; 1.4 ± 0.04) and OG (59.6 ± 5.1; 1.3 ± 0.1) were significantly higher (p < 0.05) than the control (45.4 ± 0.5; 1.1 ± 001). Similarly, the feed conversion ratio of CM (1.23 ± 0.03), TP and OG (1.30 ± 0.05) were better (p < 0.05) than the control (1.54 ± 0.05). Fish survival, feed intake and blood indices; WBC, PLT, MCV, MCH and MCHC values were not statistically different (p > 0.05) among all the treatments. Nevertheless, RBC, HGB, HCT and LYM (%) values were significantly higher in OG and TP while in CM, HGB and LYM (%) values were significantly higher (p < 0.05) compared to the control. Fish survival after S. agalactiae challenge was significantly higher (p < 0.05) in OG (70%), TP (63.3%) and CM (53.3%) compared to the control (26.3%). Dietary supplementation of CM, OG and TP significantly (p < 0.05) improved serum protein profiles, glucose, kidney and liver functions. Thus, supplementation of Nile tilapia diet with these phytobiotics is recommended to improve growth, immunity and resistance against S. agalactiae.
Immediate assessment of colorectal anastomosis with intraoperative colonoscopy: its role in reducing anastomotic complications
Authors: Yoo Ri Na , Mun Ji Yeon , Kim Jieun , Cho Hyeon-Min , Kye Bong-Hyeon , Kim Hyung Jin 
Published: November 2022

Abstract
Purpose
The use of intraoperative colonoscopy (IOC) to evaluate the integrity of newly created anastomosis has been advocated by some surgeons. However, it is still unclear whether direct visualization of fresh anastomosis can help reduce anastomotic problems. This study investigates the impact of immediate endoscopic assessment of colorectal anastomosis on anastomotic problems.
Methods
This is a retrospective cohort study conducted at a single center. Of 1210 patients undergoing radical resection for colorectal cancer from 2017 to 2021, 649 patients who underwent stapled anastomosis for left-sided colorectal cancer were included in the study. The anastomotic complications were compared between patients who underwent IOC immediately after creating the colorectal anastomosis and those who did not. Additionally, patients with subsequent intervention after the IOC assessment were compared to those without the intervention.
Results
Of 541 patients with IOC assessment, twenty-seven patients (5.0%) developed anastomotic leakage, and six (1.1%) experienced anastomotic bleeding postoperatively. The anastomotic leakage rate was similar to that in patients who did not undergo IOC (4.6%). Of the patients with IOC, 70 patients (12.9%) received reinforcement sutures to secure anastomotic stability. Of 70 patients, thirty-nine (55.7%) showed abnormal findings of mucosal edema, stapler disruption, bleeding, and air leakage in IOC. Thirty-seven patients (94.9%) who underwent reinforcement sutures did not develop postoperative anastomotic problems; however, two patients developed postoperative anastomotic leakage. Those two patients underwent reoperation due to proximal limb ischemia.
Conclusion
This study demonstrates that IOC assessment with reinforcement sutures does not imminently reduce the rate of anastomotic complications in stapled left-sided colorectal anastomosis. However, its routine use may play a role in detecting early technical failure and preventing postoperative anastomotic complications in some patients. Regarding its limitation in detecting progressive ischemia, an investigation into the indocyanine green (ICG) test combined with the endoscopic assessment would help elucidate the optimal anastomosis condition.