bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contacts with general practitioners, dentists, and medical specialists among nursing home residents: a cross-sectional study in 44 German nursing homes
Authors: Czwikla Jonas , Schmidt Annika , Schulz Maike , Gerhardus Ansgar , Schmiemann Guido , Wolf-Ostermann Karin , Gand Daniel , Friedrich Anna-Carina , Hoffmann Falk , Rothgang Heinz 
Published: January 2022

Abstract
Background
Nursing home residents have high medical care needs. Their medical care utilization is, however, lower compared to community-dwelling elderly and varies widely among nursing homes. This study quantified the utilization of general practitioners (GPs), dentists, and medical specialists among nursing homes and residents, and investigated whether dentist utilization is associated with individual and nursing home characteristics.

Methods
Forty-four nursing homes invited 2124 residents to participate in a cross-sectional study. For 10 medical specialties, data on contacts in nursing homes, practices, and by telephone in the last 12 months were assessed at individual and nursing home level. The proportion of nursing homes and residents with any form of contact, and the median number and interquartile range (IQR) of contacts among individuals with contact were determined. Using multilevel logistic regression, associations between the probability of individual dental care utilization and sex, age, LTC grade, years of residence, sponsorship, number of nursing home beds, and transport and medical escort services for consultations at a practice were investigated.

Results
The proportion of nursing homes with any form of contact with physicians ranged from 100% for GPs, dentists, and urologists to 76.7% for gynecologists and orthopedists. Among the nursing homes, 442 residents participated (20.8% response). The proportion of residents with any contact varied from 97.8% for GPs, 38.5% for neurologists/psychiatrists, and 32.3% for dentists to 3.0% for gynecologists. Only for GPs, neurologists/psychiatrists, dentists, otorhinolaryngologists, urologists, and dermatologists, the proportion was higher for nursing home contacts than for practice and telephone contacts. Among residents with any contact, the median number of contacts was highest for GPs (11.0 [IQR 7.0-16.0]), urologists (4.0 [IQR 2.0-7.0]), and neurologists/psychiatrists (3.0 [IQR 2.0-5.0]). Dentist utilization varied widely among nursing homes (median odds ratio 2.5) and was associated with higher age.

Conclusions
Almost all residents had regular contact to GPs, but only one third had contact with dentists. Lower proportions with contact were found for medical specialists, except for neurologists/psychiatrists. Reasons for the large variations in dental care utilization among nursing homes should be identified.

Trial registration
DRKS00012383 [2017/12/06].
Facilitating drug delivery in the central nervous system by opening the blood-cerebrospinal fluid barrier with a single low energy shockwave pulse
Authors: Kung Yi , Chen Kuan-Yu , Liao Wei-Hao , Hsu Yi-Hua , Wu Chueh-Hung , Hsiao Ming-Yen , Huang Abel P.-H. , Chen Wen-Shiang 
Published: January 2022

Abstract
Background
The blood-cerebrospinal fluid (CSF) barrier (BCSFB) is critically important to the pathophysiology of the central nervous system (CNS). However, this barrier prevents the safe transmission of beneficial drugs from the blood to the CSF and thus the spinal cord and brain, limiting their effectiveness in treating a variety of CNS diseases.

Methods
This study demonstrates a method on SD rats for reversible and site-specific opening of the BCSFB via a noninvasive, low-energy focused shockwave (FSW) pulse (energy flux density 0.03 mJ/mm2) with SonoVue microbubbles (2 × 106 MBs/kg), posing a low risk of injury.

Results
By opening the BCSFB, the concentrations of certain CNS-impermeable indicators (70 kDa Evans blue and 500 kDa FITC-dextran) and drugs (penicillin G, doxorubicin, and bevacizumab) could be significantly elevated in the CSF around both the brain and the spinal cord. Moreover, glioblastoma model rats treated by doxorubicin with this FSW-induced BCSFB (FSW-BCSFB) opening technique also survived significantly longer than untreated controls.

Conclusion
This is the first study to demonstrate and validate a method for noninvasively and selectively opening the BCSFB to enhance drug delivery into CSF circulation. Potential applications may include treatments for neurodegenerative diseases, CNS infections, brain tumors, and leptomeningeal carcinomatosis.
LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4
Authors: Qi Change , Liu Jianwei , Guo Pengnv , Xu Yali , Hu Jing , Han Xiaomei 
Published: January 2022

Abstract
Background
Long non-coding RNAs (lncRNAs) have been reported to be vital factors to affect the expression of genes and proteins. Also, it has been proved that the abnormal expression or mutation of lncRNAs stands as a signal of metastasis and proliferation of cancer. Nevertheless, the majority of lncRNAs still need to be explored in abundant cancers especially in oral squamous cell carcinoma (OSCC).

Methods
RT-qPCR assays were applied to test the expression of RNAs. Mechanism assays were performed to verify the combination among NORAD, TPM4 and miR-577. Also, functional assays were conducted to verify the function of RNAs on OSCC cells.

Results
LncRNA NORAD was highly expressed in OSCC tissues and cells. NORAD silencing repressed the biological behaviors of OSCC cells. MiR-577 was found in OSCC with low expression, and RIP assays illustrated that NORAD, miR-577 and TPM4 coexisted in RNA-induced silencing complexes. Rescue assays proved that the overexpression of TPM4 could recover the effect of NORAD silencing on OSCC progression.

Conclusions
It was revealed that NORAD functioned as a tumor promoter to sponge miR-577 thus elevating TPM4 in OSCC, which indicated that NORAD was worthy to be studied as a target for the treatment of OSCC.
Association analysis of SOCS3, JAK2 and STAT3 gene polymorphisms and genetic susceptibility to type 2 diabetes mellitus in Chinese population
Authors: Zhang Yang , Lin Chunwen , Chen Rong , Luo Ling , Huang Jialu , Liu Hao , Chen Weiying , Xu Jian , Yu Haibing , Ding Yuanlin 
Published: January 2022

Abstract
Aim
The association of polymorphisms in the three genes of SOCS3, JAK2 and STAT3 with genetic susceptibility to type 2 diabetes mellitus (T2DM) was explored, and its interaction with environmental factors such as hypertension and triglycerides was analyzed.

Methods
The Hardy–Weinberg balance test was used to analyze the random balance of genes in the population. The analysis of the association of SNPs with T2DM was performed using Pearson’s chi-square test. Haplotype frequency distribution, SNPs-SNPs interaction and environmental factors were analyzed by chi-square test and logistic regression.

Results
The genotype distribution of SNPs rs2280148 of the SOCS3 gene was statistically significant. The allele frequency distribution of SNPs (rs4969168/rs2280148) was statistically different. After covariate correction, the SOCS3 gene locus (rs4969168) showed an association with T2DM in additive model, while the rs2280148 locus showed an association with T2DM in all three models. The locus (rs10974914/rs10815157) allele and genotype frequency distribution of JAK2 were statistically significant. After covariate correction, two SNPs in the gene showed association with T2DM in both additive and recessive models. The distribution of genotype frequencies of SNPs rs1053005 locus in gene STAT3 was statistically significant between the two groups. In recessive genetic models, rs1053005 locus polymorphisms was associated with T2DM. Haplotype S3 (G G)/S 4 (G T) of the SOCS3 gene as well as haplotype J2 (A G)/J 3 (G C) of the JAK2 gene were closely associated with T2DM. There was an interaction between SNPs rs4969168 and SNPs rs2280148 in the SOCS3 gene. There was an interaction between the SOCS3, JAK2 and STAT3 genes and hypertension/triglycerides.

Conclusion
The SOCS3 and JAK2 genes may be associated with T2DM in the Chinese population, in which SNPs carrying the A allele (rs4969168)/G allele (rs2280148)/C allele (rs10815157) have a reduced risk of T2DM. Haplotype S3 (G G)/S 4 (G T) of the SOCS3 gene and haplotype J2 (A G)/J 3 (G C) of the JAK2 gene may be influencing factor for T2DM. The interaction between SNPs rs4969168 and SNPs rs2280148 increases the risk of T2DM. Hypertension and triglycerides may interact with SNPs of T2DM susceptibility genes.
Myxopyronin B inhibits growth of a Fidaxomicin-resistant Clostridioides difficile isolate and interferes with toxin synthesis
Authors: Brauer Madita , Herrmann Jennifer , Zühlke Daniela , Müller Rolf , Riedel Katharina , Sievers Susanne 
Published: January 2022

AbstractThe anaerobic, gastrointestinal pathogenClostridioides difficilecan cause severe forms of enterocolitis which is mainly mediated by the toxins it produces. The RNA polymerase inhibitor Fidaxomicin is the current gold standard for the therapy ofC. difficile infections due to several beneficial features including its ability to suppress toxin synthesis inC. difficile. In contrast to the Rifamycins, Fidaxomicin binds to the RNA polymerase switch region, which is also the binding site for Myxopyronin B. Here, serial broth dilution assays were performed to test the susceptibility ofC. difficileand other anaerobes to Myxopyronin B, proving that the natural product is considerably active againstC. difficileand that there is no cross-resistance between Fidaxomicin and Myxopyronin B in a Fidaxomicin-resistantC. difficilestrain. Moreover, mass spectrometry analysis indicated that Myxopyronin B is able to suppress early phase toxin synthesis inC. difficileto the same degree as Fidaxomicin. Conclusively, Myxopyronin B is proposed as a new lead structure for the design of novel antibiotics for the therapy ofC. difficileinfections.
The effect of exercise on cytokines: implications for musculoskeletal health: a narrative review
Authors: Docherty Sophie , Harley Rachael , McAuley Joseph J. , Crowe Lindsay A. N. , Pedret Carles , Kirwan Paul D. , Siebert Stefan , Millar Neal L. 
Published: January 2022

AbstractThe physiological effects of physical exercise are ubiquitously reported as beneficial to the cardiovascular and musculoskeletal systems. Exercise is widely promoted by medical professionals to aid both physical and emotional wellbeing; however, mechanisms through which this is achieved are less well understood. Despite numerous beneficial attributes, certain types of exercise can inflict significant significant physiological stress. Several studies document a key relationship between exercise and immune activation. Activation of the innate immune system occurs in response to exercise and it is proposed this is largely mediated by cytokine signalling. Cytokines are typically classified according to their inflammatory properties and evidence has shown that cytokines expressed in response to exercise are diverse and may act to propagate, modulate or mitigate inflammation in musculoskeletal health. The review summarizes the existing literature on the relationship between exercise and the immune system with emphasis on how exercise-induced cytokine expression modulates inflammation and the immune response.
Quality of life and cost consequence of delays in endovascular treatment for acute ischemic stroke in China
Authors: Ni Weiyi , Kunz Wolfgang G. , Goyal Mayank , Chen Lijin , Jiang Yawen 
Published: January 2022

Abstract
Background
Although endovascular therapy (EVT) improves clinical outcomes in patients with acute ischemic stroke, the time of EVT initiation significantly influences clinical outcomes and healthcare costs. This study evaluated the impact of EVT treatment delay on cost-effectiveness in China.

Methods
A model combining a short-term decision tree and long-term Markov health state transition matrix was constructed. For each time window of symptom onset to EVT, the probability of receiving EVT or non-EVT treatment was varied, thereby varying clinical outcomes and healthcare costs. Clinical outcomes and cost data were derived from clinical trials and literature. Incremental cost-effectiveness ratio and incremental net monetary benefits were simulated. Deterministic and probabilistic sensitivity analyses were performed to assess the robustness of the model. The willingness-to-pay threshold per quality-adjusted life-year (QALY) was set to ¥71,000 ($10,281).

Results
EVT performed between 61 and 120 min after the stroke onset was most cost-effective comparing to other time windows to perform EVT among AIS patients in China, with an ICER of ¥16,409/QALY ($2376) for performing EVT at 61–120 min versus the time window of 301–360 min. Each hour delay in EVT resulted in an average loss of 0.45 QALYs and 165.02 healthy days, with an average net monetary loss of ¥15,105 ($2187).

Conclusions
Earlier treatment of acute ischemic stroke patients with EVT in China increases lifetime QALYs and the economic value of care without any net increase in lifetime costs. Thus, healthcare policies should aim to improve efficiency of pre-hospital and in-hospital workflow processes to reduce the onset-to-puncture duration in China.
φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3
Authors: Filik Karolina , Szermer-Olearnik Bożena , Niedziółka-Jönson Joanna , Roźniecka Ewa , Ciekot Jarosław , Pyra Anna , Matyjaszczyk Irwin , Skurnik Mikael , Brzozowska Ewa 
Published: January 2022

AbstractYersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestion of contaminated food. Yersiniosis is considered a rare disease, but recent studies indicate that it is overlooked in the diagnostic process therefore the infections with this bacterium are not often identified. Reliable diagnosis of Yersiniosis by culturing is difficult due to the slow growth of the bacteria easily overgrown by other more rapidly growing microbes unless selective growth media is used. Phage adhesins recognizing bacteria in a specific manner can be an excellent diagnostic tool, especially in the diagnosis of pathogens difficult for culturing. In this study, it was shown that Gp17, the tail fiber protein (TFP) of phage φYeO3-12, specifically recognizes only the pathogenic Yersinia enterocolitica serotype O:3 (YeO:3) bacteria. The ELISA test used in this work confirmed the specific interaction of this protein with YeO:3 and demonstrated a promising tool for developing the pathogen recognition method based on phage adhesins.
Reducing infantile anemia: insight on patterns of process and outcome indicators by ethnicity and socioeconomic class during a 10-year intervention program and 5 years after
Authors: Meyerovitch Joseph , Carmi Doron , Aviner Shraga , Sherf Michael , Comaneshter Doron , Laks Yoseph , Key Calanit , Gabbay Uri , Cohen Arnon D. 
Published: January 2022

Abstract
Background
In 2005, Clalit Health Services (CHS), the largest health maintenance organization in Israel, initiated an intervention program aimed at reducing the prevalence rate of infantile anemia (IA). This study evaluated the progress made during the intervention (2005–2014) and its yield 5 years after it ended (2019).

Methods
The CHS database was retrospectively reviewed twice yearly from 2005 to 2014 for repetitive samples of children aged 9 to 18 months regarding the previous half-year interval, and a single sample in 2019. Data were collected on gender, ethnicity (Jewish/non-Jewish), socioeconomic class (SEC; low/intermediate/high), hemoglobin testing (yes/no), and hemoglobin level (if tested). Excluded were infants with documented or suspected hemoglobinopathy.

Results
At study initiation, the rate of performance of hemoglobin testing was 54.7%, and the IA prevalence rate was 7.8%. The performance rate was lower in the Jewish than the non-Jewish subpopulation. The low-SEC subpopulation had a similar hemoglobin testing rate to the high-SEC subpopulation but double the IA prevalence rate. Overall, by the end of the intervention (2014), the performance rate increased to 87.5%, and the AI prevalence rate decreased to 3.4%. In 2019, there was little change in the performance rate from the end of the intervention (88%) and the IA prevalence was further reduced to 2.7%. The non-Jewish and low-SEC subpopulations showed the most improvement which was maintained and even bettered 5 years after the intervention ended.

Conclusions
The 10-year IA intervention program introduced by CHS in 2005 led to a reduction in IA prevalence rate to about 3.5% in all sub-populations evaluated. By program end, the results in the weaker subpopulations, which had the highest prevalence of IA at baseline, were not inferior to those in the stronger subpopulations. We recommended to the Israel Ministry of Health to adopt the intervention countrywide, and we challenge other countries to consider similar interventions.
Hepatocyte generation in liver homeostasis, repair, and regeneration
Authors: Pu Wenjuan , Zhou Bin 
Published: January 2022

AbstractThe liver has remarkable capability to regenerate, employing mechanism to ensure the stable liver-to-bodyweight ratio for body homeostasis. The source of this regenerative capacity has received great attention over the past decade yet still remained controversial currently. Deciphering the sources for hepatocytes provides the basis for understanding tissue regeneration and repair, and also illustrates new potential therapeutic targets for treating liver diseases. In this review, we describe recent advances in genetic lineage tracing studies over liver stem cells, hepatocyte proliferation, and cell lineage conversions or cellular reprogramming. This review will also evaluate the technical strengths and limitations of methods used for studies on hepatocyte generation and cell fate plasticity in liver homeostasis, repair and regeneration.
Personal identification with artificial intelligence under COVID-19 crisis: a scoping review
Authors: Matsuda Shinpei , Yoshimura Hitoshi 
Published: January 2022

Abstract
Background
Artificial intelligence is useful for building objective and rapid personal identification systems. It is important to research and develop personal identification methods as social and institutional infrastructure. A critical consideration during the coronavirus disease 2019 pandemic is that there is no contact between the subjects and personal identification systems. The aim of this study was to organize the recent 5-year development of contactless personal identification methods that use artificial intelligence.

Methods
This study used a scoping review approach to map the progression of contactless personal identification systems using artificial intelligence over the past 5 years. An electronic systematic literature search was conducted using the PubMed, Web of Science, Cochrane Library, CINAHL, and IEEE Xplore databases. Studies published between January 2016 and December 2020 were included in the study.

Results
By performing an electronic literature search, 83 articles were extracted. Based on the PRISMA flow diagram, 8 eligible articles were included in this study. These eligible articles were divided based on the analysis targets as follows: (1) face and/or body, (2) eye, and (3) forearm and/or hand. Artificial intelligence, including convolutional neural networks, contributed to the progress of research on contactless personal identification methods.

Conclusions
This study clarified that contactless personal identification methods using artificial intelligence have progressed and that they have used information obtained from the face and/or body, eyes, and forearm and/or hand.
Indicators for the optimization of sustainable urban energy systems based on energy system modeling
Authors: Klemm Christian , Wiese Frauke 
Published: January 2022

AbstractBackgroundUrban energy systems are responsible for 75% of the world’s energy consumption and for 70% of the worldwide greenhouse gas emissions. Energy system models are used to optimize, benchmark and compare such energy systems with the help of energy sustainability indicators. We discuss several indicators for their basic suitability and their response to changing boundary conditions, system structures and reference values. The most suitable parameters are applied to four different supply scenarios of a real-world urban energy system.ResultsThere is a number of energy sustainability indicators, but not all of them are suitable for the use in urban energy system optimization models. Shortcomings originate from the omission of upstream energy supply chains (secondary energy efficiency), from limited capabilities to compare small energy systems (energy productivity), from excessive accounting expense (regeneration rate), from unsuitable accounting methods (primary energy efficiency), from a questionable impact of some indicators on the overall system sustainability (self-sufficiency), from the lack of detailed information content (share of renewables), and more. On the other hand, indicators of absolute greenhouse gas emissions, energy costs, and final energy demand are well suitable for the use in optimization models. However, each of these indicators only represents partial aspects of energy sustainability; the use of only one indicator in the optimization process increases the risk that other important aspects will deteriorate significantly, eventually leading to suboptimal or even unrealistic scenarios in practice. Therefore, multi-criteria approaches should be used to enable a more holistic optimization and planning of sustainable urban energy systems.ConclusionWe recommend multi-criteria optimization approaches using the indicators of absolute greenhouse gas emissions, absolute energy costs, and absolute energy demand. For benchmarking and comparison purposes, specific indicators should be used and therefore related to the final energy demand, respectively, the number of inhabitants. Our example scenarios demonstrate modeling strategies to optimize sustainability of urban energy systems.