bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk Factors and Outcome of Obstructed Labour: A Study in Rajshahi Medical College Hospital, Rajshahi, Bangladesh
Authors: Shahreen Homaira , Khatun Rokeya 
Published: March 2022

Introduction: Obstructed labour is one of the major causes of maternal mortality (8%) in Bangladesh. It is also responsible for high rate of maternal and fetal morbidity, if we can identify causes of obstructed labour, determine the outcome & complications in our country it may be helpful to find out the way to prevent this disease. Objective: The purpose of the present study was to determine the risk factors as well as to assess the outcome of obstructed labour.
Methods: This study is a prospective observational study has been done in Department of Obstetrics and Gynaecology in Rajshahi Medical College Hospital, Rajshahi, Bangladesh during July 2016 to December 2018. Hundred patients who were admitted with obstructed labour during study period were included in this study. A detailed history included socio-demographic feature, obstetric history, features of obstruction, intrapartum events were recorded to detect risk factors. Condition of patients, mode of delivery, preoperative and postoperative complications, maternal and fetal outcomes were recorded.
Results: This study was one hundred (100) cases with features of obstructed labour were selected. The highest frequency was found among the unbooked, primigravid patients that were illiterate or only having primary education level. 84% in primi-gravidae and 10% in multi gravidae had come from medium socio-economic group and only 2% in case of primigravidae came from good economic classes. Revealed that 56% in primigravidae and 68% in multi gravidae of study population did not cross primary education level. the causative factors of obstructed labour, 46% in primigravidae and 42% multi gravidae cases were due to cephalopelvic disproportion with varying degrees of contracted pelvis, 52% in primigravidae and 58% multi gravidae cases caused by malpositions and malpresentations, 2% in primigravidae was due to cervical fibroid. The medical causes of obstructed labour in the study population were cephalopelvic disproportion 42-46%. One patient died due to post-partum hemorrhage among 10(10.0%) cases.
Conclusion: In this study the incidence of obstructed labour was very high. The commonest cause was cephalopelvic disproportion followed by fetal malpostion and malpresentation.
TAJ 2021; 34: No-2: 80-89
Prosedur Clearance In Dan Clearance Out Kapal Milik PT. Salam Pacific Indonesia Lines
Authors: Bellin Fattah Bellin Fattah , Iswanto Iswanto , Astriawati Ningrum , Widyanto Handoyo 
Published: March 2022

Tujuan dari penelitian ini adalah untuk mengetahui prosedur clearance in dan clearance out kapal milik PT. Salam Pacific Indonesia Lines di Pelabuhan Tanjung Priok. Penelitian ini menggunakan metode pendekatan deskriptif kualitatif. Metode pengumpulan data terbagi menjadi 4 bagian yaitu: metode observasi secara langsung di lapangan,) metode interview atau wawancara dengan kepala cabang beserta staf atau karyawan PT. Salam Pacific Indonesia Lines (SPIL) Cabang Jakarta, metode documenter terkait dengan dokumen yang diperlukan pada saat proses clearance in dan clearance out kapal, metode studi pustaka yang berhubungan dengan prosedur clearance in dan clearance out kapal. Dari hasil penelitian disimpulkan bahwa di dalam kegiatan clearance in dan clearance out kapal pihak dinas luar PT. Salam Pacific Indonesia Lines diharuskan melakukan kegiatan tepat dan cepat. Prosedur sudah dilakukan dengan baik. Semua kegiatan sudah terorganisir untuk mengurangi kesalahan saat melakukan kegiatan clearance in dan clearance out kapal tersebut.
Profiling Temporal Changes of the Pineal Transcriptomes at Single Cell Level Upon Neonatal HIBD
Authors: Ding Xin , Pan Tao , Tian Qiuyan , Huang Wenxi , Hayashi Lauren S , Liu Qin , Li Fuyong , Xu Li-Xiao , Miao Po , Yang Xiaofeng , Sun Bin , Feng Chen-Xi , Feng Xing , Li Mei , Huang Jian 
Published: March 2022

Neonatal hypoxic-ischemic brain damage (HIBD) often results in various neurological deficits. Among them, a common, yet often neglected, symptom is circadian rhythm disorders. Previous studies revealed that the occurrence of cysts in the pineal gland, an organ known to regulate circadian rhythm, is associated with circadian problems in children with HIBD. However, the underlying mechanisms of pineal dependent dysfunctions post HIBD remain largely elusive. Here, by performing 10x single cell RNA sequencing, we firstly molecularly identified distinct pineal cell types and explored their transcriptome changes at single cell level at 24 and 72 h post neonatal HIBD. Bioinformatic analysis of cell prioritization showed that both subtypes of pinealocytes, the predominant component of the pineal gland, were mostly affected. We then went further to investigate how distinct pineal cell types responded to neonatal HIBD. Within pinealocytes, we revealed a molecularly defined β to α subtype conversion induced by neonatal HIBD. Within astrocytes, we discovered that all three subtypes responded to neonatal HIBD, with differential expression of reactive astrocytes markers. Two subtypes of microglia cells were both activated by HIBD, marked by up-regulation ofCcl3. Notably, microglia cells showed substantial reduction at 72 h post HIBD. Further investigation revealed that pyroptosis preferentially occurred in pineal microglia through NLRP3-Caspase-1-GSDMD signaling pathway. Taken together, our results delineated temporal changes of molecular and cellular events occurring in the pineal gland following neonatal HIBD. By revealing pyroptosis in the pineal gland, our study also provided potential therapeutic targets for preventing extravasation of pineal pathology and thus improving circadian rhythm dysfunction in neonates with HIBD.
Exosome-Derived From Sepsis Patients' Blood Promoted Pyroptosis of Cardiomyocytes by Regulating miR-885-5p/HMBOX1
Authors: Tu Guo-wei , Ma Jie-fei , Li Jia-kun , Su Ying , Luo Jing-chao , Hao Guang-wei , Luo Ming-hao , Cao Yi-rui , Zhang Yi , Luo Zhe 
Published: March 2022

BackgroundSeptic myocardial depression has been associated with increased morbidity and mortality. miR-885-5p has been shown to regulate cell growth, senescence, and/or apoptosis. Published studies demonstrated that Homeobox-containing protein 1 (HMBOX1) inhibits inflammatory response, regulates cell autophagy, and apoptosis. However, the role of miR-885-5p/HMBOX1 in sepsis and septic myocardial depression and the underlying mechanism is not fully understood.Materials and MethodsExosomes (exos) derived from sepsis patients (sepsis-exos) were isolated using ultracentrifugation. Rats were subjected to cecal ligation and puncture surgery and treated with sepsis-exos. HMBOX1 was knocked down or overexpressed in AC16 cells using lentiviral plasmids carrying short interfering RNAs targeting human HMBOX1 or carrying HMBOX1 cDNA. Cell pyroptosis was measured by flow cytometry. The secretion of IL-1β and IL-18 was examined by ELISA kits. Quantitative polymerase chain reaction (PCR) or western blot was used for gene expression.ResultsSepsis-exos increased the level of miR-885-5p, decreased HMBOX1, elevated IL-1β and IL-18, and promoted pyroptosis in AC16 cells. Septic rats treated with sepsis-exos increased the serum inflammatory cytokines is associated with increased pyroptosis-related proteins of hearts. MiR-885-5p bound to the three prime untranslated regions of HMBOX1 to negatively regulate its expression. Overexpressing HMBOX1 reversed miR-885-5p-induced elevation of inflammatory cytokines and upregulation of NLRP3, caspase-1, and GSDMD-N in AC16 cells. The mechanistic study indicated that the effect of HMBOX1 was NF-κB dependent.ConclusionSepsis-exos promoted the pyroptosis of AC16 cells through miR-885-5p via HMBOX1. The results show the significance of the miR-885-5p/HMBOX1 axis in myocardial cell pyroptosis and provide new directions for the treatment of septic myocardial depression.
Dynamics and Functional Interplay of Nonhistone Lysine Crotonylome and Ubiquitylome in Vascular Smooth Muscle Cell Phenotypic Remodeling
Authors: Cao Shan-Hu , Chen Zhi-Huan , Ma Ru-Yuan , Yue Lin , Jiang Han-Mei , Dong Li-Hua 
Published: March 2022

BackgroundThe crotonylation of histones is discovered of late as one of the post-translational modifications (PTMs) that can regulate gene expression. However, the function of crotonylation on nonhistone proteins in vascular smooth muscle cells (VSMCs) is unclear. Here, we aim to find the cellular characteristics of crotonylated nonhistone proteins and the cross talk with ubiquitinated proteins in VSMC phenotypic remodeling using the modified omics and proteomic analysis.MethodsWe performed the modified omics and proteomic analysis of VSMCs before and after the stimulation with platelet-derived growth factor-BB (PDGF-BB). The crotonylated and ubiquitinated pan-antibody was used to enrich proteins and then subjected to a high-throughput mass spectrometry analysis. The enrichment analysis was performed within differentially modified proteins in regard to GO terms, KEGG, and protein domains.ResultsAs a result, there were 2,138 crotonylation sites in 534 proteins and 1,359 ubiquitination sites corresponding to 657 proteins. These crotonylated proteins detected after PDGF-BB stimulation might be involved in various vital cellular pathways and carry out important functions in VSMCs. Some of them closely took part in significant physiological processes of VSMC phenotypic remodeling, including glycolysis/gluconeogenesis, vascular smooth muscle contraction, and the PI3K-Akt signaling pathway. Furthermore, the KEGG pathway enrichment analysis showed the involvement of ubiquitinated proteins in the physiological processes of VSMC phenotypic remodeling, including glycolysis/gluconeogenesis, vascular smooth muscle contraction, RAS signaling pathway, or the PI3K-Akt signaling pathway. A cross talk analysis showed that there were 199 sites within the 177 proteins modified by crotonylation and ubiquitination simultaneously. Protein–protein interaction (PPI) network analysis indicated that crotonylated and ubiquitinated proteins play an important role in cellular bioprocess commonly and possibly have a synergistic effect.ConclusionIn summary, our bioinformatics analysis shows that the crotonylation and ubiquitination of nonhistone proteins play an essential role in VSMC phenotypic transformation induced by PDGF-BB stimulation. The cross talk between crotonylation and ubiquitination in glycolysis is possibly a novel mechanism during VSMC phenotypic remodeling.
The Efficacy and Safety of Phase I Cardiac Rehabilitation in Patients Hospitalized in Cardiac Intensive Care Unit With Acute Decompensated Heart Failure: A Study Protocol for a Randomized, Controlled, Clinical Trial
Authors: Wu Linjing , Li Jiahua , Chen Linjian , Xue Mengmeng , Zheng Yamin , Meng Fanqi , Jiang Hongfei , Shi Zaixing , Zhang Peng , Dai Cuilian 
Published: March 2022

BackgroundCardiac rehabilitation (CR) improves outcomes in patients with heart failure. However, data on CR efficacy in patients with acute decompensated heart failure is limited. This study is designed to assess the efficacy and safety of CR in patients hospitalized in cardiac intensive care unit (CICU) with acute decompensated heart failure (ADHF).MethodsThis is a single-center, randomized controlled, single-blind clinical trial. A total of 120 participants hospitalized in CICU with ADHF will be randomly allocated in the ratio of 1:1 to two groups: CR group and control group. Participants will receive tailored and progressive CR intervention or attention control. The CR intervention include personalized breathing training, small muscle group resistance training, and aerobic endurance training based on the physical fitness assessment results. The subjects will receive the CR training for 5 days and will be followed up for 6 months. The primary endpoints are the score of the short physical performance battery (SPPB) and 6-month all-cause rehospitalization. The secondary endpoints include cardio-pulmonary function, activities of daily living (ADL), in-hospital mortality rate and 6-month all-cause mortality rate.DiscussionThis randomized, controlled, clinical trial will assess whether CR improves physical function and reduces rehospitalization in patients hospitalized in CICU with ADHF. The results will provide further research-based evidence for the clinical application of CR in patients with ADHF.Trial RegistrationChinese Clinical Trial Registry ChiCTR2100050151. Registered on 19 August 2021.
Association Between Non-alcoholic Fatty Liver Disease and Risk of Stroke: A Systematic Review and Meta-Analysis
Authors: Wang Meng , Zhou Ben-Gang , Zhang Yi , Ren Xi-Fang , Li Ling , Li Bo , Ai Yao-Wei 
Published: March 2022

Background/ObjectivesRecent observational studies have explored the association between non-alcoholic fatty liver disease (NAFLD) and stroke with controversial results. We therefore performed a meta-analysis to investigate this possible association.MethodsPubMed, EMBASE and Web of Science database were searched from inception until December 2019, and updated on May 2021. Random-effects meta-analyses were performed by generic inverse variance method. Subgroup and sensitivity analyses were also conducted. The PROSPERO registered number of this study is CRD42020167330.ResultsTwenty observational (15 cohort, 4 cross-sectional, and 1 case-control) studies with 17,060,388 participants were included in the meta-analysis. Meta-analysis of data from 18 studies with 17,031,672 participants has shown that NAFLD was associated with mildly increased risk of stroke (OR = 1.18, 95% CI: 1.08–1.30, P = 0.0005). Similar results were observed in most of the subgroup analyses we performed. Sensitivity analyses did not alter these findings. Meta-analysis of data from 3 studies with 29,614 participants has shown that insufficient evidence to support the proposed association between NAFLD-fibrosis and an increased risk of stroke.ConclusionsWe found that NAFLD was associated with increased risk of stroke. However, there was insufficient evidence to support the proposed association between NAFLD-fibrosis and an increased risk of stroke. To better understand any association, future well-designed prospective studies that take fully account of specific population, type of stroke, and confounding factors are warranted.Systematic Review RegistrationUnique Identifier: CRD42020167330.
Trends in Transcatheter Edge-to-Edge Mitral Valve Repair Over a Decade: Data From the MiTra ULM Registry
Authors: Nita Nicoleta , Schneider Leonhard , Dahme Tilman , Markovic Sinisa , Keßler Mirjam , Rottbauer Wolfang , Tadic Marijana 
Published: March 2022

ObjectiveThis study sought to determine the potential change in trends in the baseline characteristics of patients with symptomatic severe mitral regurgitation who underwent transcatheter edge-to-edge mitral valve repair (M-TEER) over the last decade in a high-volume center.MethodologyThe investigation included 942 symptomatic patients with moderate-to-severe and severe mitral regurgitation who underwent transcatheter edge-to-edge repair (TEER) at our institution between January 2010 and March 2021. Patients were divided into quintiles and compared separately.ResultsPatients treated in the last quintile had significantly lower surgical risk (Euro Score 7.2 ± 6.8% in the last quintile vs. 10.9 ± 9.4% in the first quintile, p &lt; 0.001), better New York Heart Association (NYHA) status (NYHA IV 14% in the last quintile vs. 40% in the first quintile, p &lt; 0.001), lower NT-pro-BNP, and smaller left ventricle diameter than patients who were treated in the first quintile. There was no difference in age between quintiles. However, an invasive hemodynamic assessment did not show significant changes over the last decade (sPAP 51.35 ± 16.2 mmHg in the first quintile vs. 51.02 ± 14.5 mmHg in the last quintile, p = 0.90, pulmonary capillary wedge V wave 30.7 ± 14.8 mmHg in the first quintile vs. 27.4 ± 10.3 mmHg in the last quintile, p = 0.40). There is a significant trend of a gradually increasing proportion of patients with degenerative mitral regurgitation (MR) over the last 10 years (p &lt; 0.001). The experience gained in the M-TEER procedure brought a significant reduction in fluoroscopy time and hospitalization duration. Medical therapy significantly changed over the last decade in terms of higher use of angiotensin receptor blockers (ARBs), lower use of angiotensin-converting enzyme inhibitors (ACEIs), and the introduction of angiotensin receptor-neprilysin inhibitors (ARNIs).ConclusionPatients undergoing the M-TEER procedure nowadays have lower surgical risk and are treated before they develop a significant left ventricular (LV) remodeling than before. The increasing expertise on the procedure over the last decade led to a rising number of patients with complex degenerative pathology being treated.
Echocardiographic Parameters to Predict Atrial Fibrillation in Clinical Routine—The EAHsy-AF Risk Score
Authors: Sieweke Jan-Thorben , Hagemus Jan , Biber Saskia , Berliner Dominik , Grosse Gerrit M. , Schallhorn Sven , Pfeffer Tobias Jonathan , Derda Anselm A. , Neuser Jonas , Bauersachs Johann , Bavendiek Udo 
Published: March 2022

BackgroundEchocardiographic parameters representing impaired left atrial (LA) function and remodeling are of high value to predict atrial fibrillation (AF). This study aimed to develop a prediction model for AF easily to apply in clinical routine containing echocardiographic parameters associated with LA remodeling and—function.Methods and ResultsThis monocentric, semi-blinded, controlled analysis included 235 patients to derive a prediction model. This prediction model was tested in a validation cohort encompassing 290 cardiovascular inpatients. The derivation and validation cohort included 54 (23%) and 66 (23%) patients with AF, respectively. Transthoracic echocardiography, comprising parameters indicating left atrial remodeling [septal/lateral total atrial conduction time (s/l PA-TDI)] and left atrial volume indexed to a' (LAVI/a') was performed in each patient. Based on multivariable regressions analysis, four variables were enclosed into the EAHsy (Echocardiography, Age, Hypertension)-AF risk score for AF prediction: Hypertension, Age, LAVI/a‘ and septal PA-TDI. In the validation cohort discrimination was strong (C-statistic 0.987, 95%CI 0.974–0.991) with an adequately performed calibration. The EAHsy-AF risk score was associated with a more precise prediction of AF in comparison to commonly used AF-scores (CHADS2-, ATLAS-, ARIC-, CHARGE-AF score).ConclusionThe EAHsy-AF-Score containing age, hypertension and echocardiographic parameters of atrial dysfunction and remodeling precisely predicts the incidence of AF in a general population of patients with cardiovascular disease. The EAHsy-AF risk score may enable more selective rhythm monitoring in specific patients at high risk for AF.
All-Solid-State High-Voltage Supercapacitors Using an Ionic Plastic Crystal-Based Electrolyte
Authors: Yoshizawa-Fujita Masahiro , Kubota Satoshi , Ishimoto Shuichi 
Published: March 2022

Recently, ionic plastic crystals (IPCs) have been actively investigated to develop all-solid-state rechargeable batteries, such as lithium-ion batteries. Herein, we report supercapacitors assembled with mesoporous carbon electrodes and an IPC electrolyte, N-ethyl-N-methylpyrrolidinium bis(fluorosulfonyl)amide ([C2mpyr][FSA]). [C2mpyr][FSA] with a 10 mol% lithium bis(fluorosulfonyl)amide (LiFSA) dopant was used as the solid electrolyte in the supercapacitors. The charge–discharge tests of the supercapacitors were performed at various C-rates in the voltage range of 0–2.5 V at 25°C. The capacitance of the cells was 12.3 Fg−1 at a lower C rate (1 C = 8.9 mA g−1). The capacitance retention of the supercapacitors was maintained at approximately 100% up to 20 C, which was comparable to that of the cells containing organic electrolyte solutions. The advantage of using solid electrolytes was the fabrication of bipolar cells using two pairs of mesoporous carbon electrodes and a [C2mpyr][FSA]/LiFSA composite. The charge–discharge tests of the bipolar cells were also performed in the voltage range of 0–5.0 V at 25°C. The capacitance of the bipolar cells was 6.4 Fg−1 at a lower C rate. The bipolar cells exhibited a typical charge–discharge profile for 1,000 cycles, confirming their stable cyclic performance. Thus, IPC electrolytes are interesting materials for developing all-solid-state high-voltage supercapacitors.
Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease
Authors: Zhang Yuepeng , Xia Rongyao , Lv Meiyu , Li Zhiheng , Jin Lingling , Chen Xueda , Han Yaqian , Shi Chunpeng , Jiang Yanan , Jin Shoude 
Published: March 2022

ObjectiveThis study aims to identify clinically relevant diagnostic biomarkers in chronic obstructive pulmonary disease (COPD) while exploring how immune cell infiltration contributes towards COPD pathogenesis.MethodsThe GEO database provided two human COPD gene expression datasets (GSE38974 and GSE76925; n=134) along with the relevant controls (n=49) for differentially expressed gene (DEG) analyses. Candidate biomarkers were identified using the support vector machine recursive feature elimination (SVM-RFE) analysis and the LASSO regression model. The discriminatory ability was determined using the area under the receiver operating characteristic curve (AUC) values. These candidate biomarkers were characterized in the GSE106986 dataset (14 COPD patients and 5 controls) in terms of their respective diagnostic values and expression levels. The CIBERSORT program was used to estimate patterns of tissue infiltration of 22 types of immune cells. Furthermore, the in vivo and in vitro model of COPD was established using cigarette smoke extract (CSE) to validated the bioinformatics results.Results80 genes were identified via DEG analysis that were primarily involved in cellular amino acid and metabolic processes, regulation of telomerase activity and phagocytosis, antigen processing and MHC class I-mediated peptide antigen presentation, and other biological processes. LASSO and SVM-RFE were used to further characterize the candidate diagnostic markers for COPD, SLC27A3, and STAU1. SLC27A3 and STAU1 were found to be diagnostic markers of COPD in the metadata cohort (AUC=0.734, AUC=0.745). Their relevance in COPD were validated in the GSE106986 dataset (AUC=0.900 AUC=0.971). Subsequent analysis of immune cell infiltration discovered an association between SLC27A3 and STAU1 with resting NK cells, plasma cells, eosinophils, activated mast cells, memory B cells, CD8+, CD4+, and helper follicular T-cells. The expressions of SLC27A3 and STAU1 were upregulated in COPD models both in vivo and in vitro. Immune infiltration activation was observed in COPD models, accompanied by the enhanced expression of SLC27A3 and STAU1. Whereas, the knockdown of SLC27A3 or STAU1 attenuated the effect of CSE on BEAS-2B cells.ConclusionSTUA1 and SLC27A3 are valuable diagnostic biomarkers of COPD. COPD pathogenesis is heavily influenced by patterns of immune cell infiltration. This study provides a molecular biology insight into COPD occurrence and in exploring new therapeutic means useful in COPD.