bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retinoblastoma-binding protein 5 regulates H3K4 methylation modification to inhibit the proliferation of melanoma cells by inactivating the Wnt/β-catenin and epithelial-mesenchymal transition pathways
Authors: Yang Zhiqin , Jia Yue , Wang Shaojia , Zhang Yongjun , Fan Wen , Wang Xin , He Liang , Shen Xiaoyu , Yang Xiangqun , Zhang Yi , Yang Hongying 
Published: October 2022

Abstract
Background
Histone 3 lysine 4 methylation (H3K4me), especially histone 3 lysine 4 trimethylation (H3K4me3), is one of the most extensively studied patterns of histone modification and plays crucial roles in many biological processes. However, as a part of H3K4 methyltransferase that participates in H3K4 methylation and transcriptional regulation, retinoblastoma-binding protein 5 (RBBP5) has not been well studied in melanoma cancer. The present study sought to explore RBBP5-mediated H3K4 histone modification and the potential mechanisms in melanoma.
Methods
RBBP5 expression in melanoma and nevi specimens was detected by immunohistochemistry. Western blotting was performed for three pairs of melanoma cancer tissues and nevi tissues. In vitro and in vivo assays were used to investigate the function of RBBP5. The molecular mechanism was determined using RT-qPCR, western blotting, ChIP assays, and co-IP assays.
Results
Our study showed that RBBP5 was significantly downregulated in melanoma tissue and cells compared with nevi tissues and normal epithelia cells (P < 0.05). Reducing RBBP5 in human melanoma cells leads to H3K4me3 downregulation and promotes cell proliferation, migration, and invasion. On the one hand, we verified that WD repeat and SOCS box containing protein 2(WSB2) was an upstream gene of RBBP5-mediated H3K4 modification, which could directly bind to RBBP5 and negatively regulate its expression. On the other hand, we also confirmed that p16 (a cancer suppressor gene), was a downstream target of H3K4me3, the promoter of which can directly bind to H3K4me3. Mechanistically, our data revealed that RBBP5 inactivated the Wnt/β-catenin and epithelial-mesenchymal transition (EMT) pathways (P < 0.05), leading to melanoma suppression.
Conclusion
Histone methylation is rising as an important factor affecting tumorigenicity and tumour progression. Our findings verified the significance of RBBP5-mediated H3K4 modification in melanoma and the potential regulatory mechanisms of melanoma proliferation and growth, suggesting that RBBP5 is a potential therapeutic target for the treatment of melanoma.
CRISPR-cas9 Screening Identified Lethal Genes Enriched in Hippo Kinase Pathway and of Prognosis Significance in primary Low-grade Glioma
Authors: Mijiti Maimaitili , Maimaiti Aierpati , Chen Xiaoqing , Tuersun Maidina , Zhu Guohua , Wu Hao , Li Yandong , Turhon Mirzat , Abulaiti Aimitaji , Maimaitiaili Nuerailijiang , Yiming Nadire , Kasimu Maimaitijiang , Wang Yongxin 
Published: October 2022

Abstract
Background
Lethal genes in low-grade gliomas (LGGs) may have an essential prognostic significance and therefore need to be systematically analyzed. The purpose of this study is to analyze essential genes correlated with cell survival by evaluating CRISPR-cas9 screening data, leading to the identification of novel treatment targets for patients.
Methods
In this study, genes related to cell viability from the Depmap CRISPR-cas9 screen were intersected to differentially expressed genes (DEGs) between tumor and GTEx normal tissues from TCGA. The LASSO regression method was used to construct a signature that used to anticipate overall survival in patients with LGG. An evaluation of the signature was carried out using both multivariate and univariate Cox regression. Then, we determined which key pathways were modulated by this signature by comparing DEGs between low- and high-risk patients. WGCNA was conducted to identify modules associated with high-risk. In this study, we also performed enrichment analysis to identify pathways mediated by DEGs, overlapping genes, and genes shared in the WGCNA. Finally, we used to western blot, qRT-PCR and IHC to detect the expression of hub genes.
Results
Using the TCGA database, 145 oncogenes were identified as overexpressed. These genes were intersected with lethal genes identified in the Depmap database, which are enriched in Hippo pathways. A total of 19 genes were used to construct the gene signature by means of LASSO regression. The median risk score (0.752324) was employed to distinguish between low- and high-risk patients. The patients with high-risk characteristics showed a shorter OS duration in the internal training, the internal validation, and the external validation datasets. Ultimately, the Hippo signaling pathway was the predominantly enriched pathway in 145 genes, DEGs, and 3 modular genes in WGCNA. Finally, we found differences of hub genes expression in different clinical samples. Importantly, protein and mRNA expression of REP65 was significantly up-regulated in tumor cells both in the public cohort and our cohort.
Conclusion
The hippo signaling pathway detected based on CRISPR-cas9 screening is a critical regulator of viability and tumor proliferation and therefore is an innovative new target for treating cancerous brain tumors, including LGG.
Learning heterogeneous delays in a layer of spiking neurons for fast motion detection
Authors: Grimaldi Antoine , Perrinet Laurent 
Published: October 2022

Abstract
The response of a biological neuron depends on the precise timing of afferent spikes. This temporal aspect of the neuronal code is essential in understanding information processing in neurobiology and applies particularly well to the output of neuromorphic hardware such as event-based cameras. However, most artificial neuronal models do not take advantage of this minute temporal dimension. Inspired by this neuroscientific observation, we develop a model for the efficient detection of temporal spiking motifs based on a layer of neurons with heterogeneous delays which we apply to the computer vision task of motion detection. Indeed, the variety of synaptic delays on the dendritic tree allows to synchronize synaptic inputs as they reach the basal dendritic tree. We show this can be formalized as a time-invariant logistic regression which can be trained using labeled data. We apply this model to solve the specific computer vision problem of motion detection, and demonstrate its application to synthetic naturalistic videos transformed into event streams similar to the output of event-based cameras. In particular, we quantify how the accuracy of the model can vary with the total computational load. This end-to-end event-driven computational brick could help improve the performance of future Spiking Neural Network (SNN) algorithms and their prospective use in neuromorphic chips.
2p25.3 microduplications involving MYT1L: further phenotypic characterization through an assessment of 15 new cases and a literature review
Authors: Bouassida Malek , Egloff Matthieu , Levy Jonathan , Chatron Nicolas , Bernardini Laura , Guyader Gwenael Le , Tabet Anne-Claude , Schluth-Bolard Caroline , Brancati Francesco , Giuffrida Maria , Dard Rodolphe , Clorennec Juliette , Coursimault Juliette , Vialard François , Herve Bérénice 
Published: October 2022

Abstract
Microduplications involving the MYT1L gene have mostly been described in series of patients with isolated schizophrenia. However, few reports have been published, and the phenotype has still not been well characterized. We sought to further characterize the phenotypic spectrum of this condition by describing the clinical features of patients with a pure 2p25.3 microduplication that included all or part of MYT1L. Through a French national collaboration and a literature review, we assessed a large cohort of patients (n = 43) with pure 2p25.3 microduplications identified by chromosomal microarray analysis. For each case, we recorded clinical data, the microduplication size, and the inheritance pattern. The clinical features were variable and included developmental and speech delays (33%), autism spectrum disorder (23%), mild-to-moderate intellectual disability (21%), schizophrenia (21%), or behavioral disorders (16%). Eleven patients did not have an obvious neuropsychiatric disorder. The microduplications ranged from 62.4 kb to 3.8 Mb in size and led to either duplication of all or part of MYT1L. There were seven cases of intragenic duplication. The inheritance pattern was available for 18 patients: the microduplication was inherited in 13 cases, and all but one of the parents had a normal phenotype. Our comprehensive review and expansion of the phenotypic spectrum associated with 2p25.3 microduplications involving MYT1L (previously linked to schizophrenia) should help clinicians to better assess, counsel and manage affected individuals. MYT1L microduplications are characterized by a spectrum of neuropsychiatric phenotypes with incomplete penetrance and variable expressivity, which are probably due to as-yet unknown genetic and nongenetic modifiers.
Corner milling force prediction and improvement method of aviation thin-walled structural parts
Authors: Chen Zhitao , Yue Caixu , Liu Xianli , Liang Steven Y. 
Published: October 2022

Abstract
Corner is one of the typical characteristics of aerospace monolithic components. In order to achieve a stable processing environment and reduce the mutation of milling force in the process of machining, the manufacturer has been searching a method to control the milling force of corner machining. Based on the cutting contact relationship and the instantaneous chip thickness, the milling force prediction model of corner machining is established. By analyzing different types of corner contact relationship, the influence of cutting contact angle and cutting arc length on milling force is established. The corner feed rate iterative calculation method is used to improve the corner milling force mutation. The corner milling experiment proves that the proposed method can not only effectively control the corner milling force, but also improve the surface quality of the bottom surface of the workpiece. The proposed method is suitable for automatic programming of multi-corner pocket machining and provides theoretical support for stable machining of frame part.
In-Situ Quality Assessment and Control in Additive Manufacturing Processes Using Laser Surface Profilometer and Deep Learning Techniques
Authors: Akhavan Javid , Lyu Jiaqi , Manoochehri Souran 
Published: October 2022

Abstract
Additive Manufacturing (AM) has become one of the most popular manufacturing techniques in various fields. Their layer-by-layer printing process allows an easier fabrication of complex geometries. However, the quality and accuracy of fabricated artifacts in these techniques have low repeatability. In the era of Industry 4.0 by using the emerging sensory and data processing capabilities such as Laser Surface Profilometer (LSP) and Deep Learning (DL), it is possible to improve the repeatability and quality of AM processes. This work presents an in-situ quality assessment and improvement using LSP for data acquisition and DL for data processing and decision making. The utilized LSP module generates a point cloud dataset containing information about the top surface geometry and quality. Once the point cloud data is preprocessed, an improved deep Hybrid Convolutional Auto-Encoder decoder (HCAE) model is used to perform the artifact's quality measurement and statistical representation. The HCAE model's statistical representation is comprised of 9*9 segments, each including four channels with the segment's probability to contain one of four labels, 1) Under-printed region, 2) Normally printed region, 3) Over-printed region, 4) Empty region. This data structure plays a significant role in determining the commands needed to optimize the fabrication process. The implemented HCAE model's accuracy and repeatability were measured by a multi-label multi-output metric developed in this study. The assessments made by HCAE are then used to perform an in-situ process adjustment by manipulating the future layer's fabrication through the G-code modification. By adjusting the machine's print speed and feedrate, the control algorithm exploits the next layer deposition, segment by segment. The algorithm is then tested with two manually defected settings, one part with severe under extrusion and one with over extrusion parameters. Both test artifacts' quality advanced significantly and converged to an acceptable state by four iterations.
Son's contribution to mother's fitness can overcome the twofold cost of sex
Authors: Yasui Yukio , Hasegawa Eisuke 
Published: October 2022

Abstract
Sexual reproduction requires mating partners and transmits only half of their genomes to an offspring1,2. However, because the sex-controlling allele is fixed at the origin of gametic sex, where each gamete provided half amount of necessary resource (isogamy), the genome dilution cost disappears3. In the traditional view1,2, derivative anisogamy (bipolarization into egg and sperm) must have half the population growth rate of asexual thelytoky because males laying no eggs are not included in population size1,2. The present dominance of anisogamy despite this "twofold cost of male production" is the greatest enigma in evolutionary biology1,2,4-6. Sex has various advantages6-27, but none overcome this cost22. Why can anisogamy become dominant? Here, we show that a son's mating with multiple females contributes to his mother's fitness. Although only females contribute egg resources in anisogamy, at the lineage level, males from a particular lineage parasitize the egg resources of the other lineages, and their own lineage's eggs are fertilized by sperm from the other lineages. Considering it reciprocal altruism between lineages, anisogamy can offset male-production costs by yielding more grandchildren than thelytoky. Our simulations prove that anisogamy is evolutionarily stable against isogamy and thelytoky. The significance of males, a question since Aristotle's time, is clearly answered.
Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells
Authors: Choa John Benson Dy , Sasaki Tadahiro , Kajiura Hiroyuki , Ikuta Kazuyoshi , Fujiyama Kazuhito , Misaki Ryo 
Published: October 2022

Abstract
Immunoglobulin A (IgA) has been showing potential as a new therapeutic antibody. However, recombinant IgA suffers from low yield. Supplementation of the medium is an effective approach to improving the production and quality of recombinant proteins. In this study, we adapted IgA1-producing CHO-K1 suspension cells to a high concentration (150 mM) of different disaccharides, namely sucrose, maltose, lactose, and trehalose, to improve the production and quality of recombinant IgA1. The disaccharide-adapted cell lines had slower cell growth rates, but their cell viability was extended compared to the nonadapted IgA1-producing cell line. Glucose consumption was exhausted in all cell lines except for the maltose-adapted one, which still contained glucose even after the 9th day of culturing. Lactate production was higher among the disaccharide-adapted cell lines. The specific productivity of the maltose-adapted IgA1-producing line was 4-fold that of the nonadapted line. In addition, this specific productivity was higher than in previous productions of recombinant IgA1 with a lambda chain. Lastly, secreted IgA1 aggregated in all cell lines, which may have been caused by self-aggregation. These results suggest that a high concentration of disaccharide-supplemented induced hyperosmolarity in the IgA1-producing CHO-K1 cell lines. In addition, the maltose-adapted CHO-K1 cell line benefited from having an additional source of carbohydrate.
Estrogen-related genes identified as novel prognostic indicators in papillary thyroid cancer
Authors: Zeng Yu , Ma Weike , Li Lijuan , Zhuang Gaojian , Luo Guoqing , Zhou Hong , Hao Weijing , Liu Yu , Guo Fengli , Tian Mengran , Ruan Xianhui , Gao Ming , Zheng Xiangqian 
Published: October 2022

Abstract
Papillary thyroid cancer (PTC) is one of the most common malignant tumors in female, and estrogen can affect its progression. However, the targets and mechanisms of estrogen action in PTC remain unclear. Therefore, this study focuses on the relationship between estrogen-related genes (ERGs) expression and prognosis in PTC, particularly neuropeptide U (NMU), and its important role in the development of PTC. We first downloaded expression data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases for PTC and normal tissue and identified differentially expressed genes (DEGs), which were predominantly enriched for ERGs. Then, we identified ERGs that contributed most to PTC prognosis based on univariate Cox regression and Lasso Cox analysis. We filtered out Transducer of ERBB2 1 (TOB1), trefoil factor 1 (TFF1), phospholipase A and acyltransferase 3 (PLAAT3), NMU, kinesin family member 20A (KIF20A), DNA topoisomerase II alpha (TOP2A), tetraspanin 13 (TSPAN13), and carboxypeptidase E (CPE). In addition, we explored the effect of NMU on the proliferation of PTC cells by in vitro experiments, confirmed high NMU expression in PTC and showed that the proliferative capacity of PTC cells was significantly reduced with NMU knockdown. Moreover, the phosphorylation levels of the Kirsten rat sarcoma virus (KRAS) signaling pathway were significantly lower with NMU knockdown. These results suggest that ERGs, especially NMU, may be novel prognostic indicators in PTC.
Time-course of heart rate variability after total hip arthroplasty
Authors: Frandsen Mikkel Nicklas , Varnum Claus , Foss Nicolai Bang , Mehlsen Jesper , Kehlet Henrik 
Published: October 2022

Abstract
Purpose
Heart rate variability (HRV) is a measure of the autonomic nervous system function and possibly related to postoperative outcome. Despite several HRV studies in different surgical settings, optimal indices and timepoints for measuring have not been adequately determined. Consequently, there is a need for detailed descriptive procedure-specific studies on the time-course of perioperative HRV within a modern fast-track surgical setting.
Method
We measured HRV continuously in 24 patients from 4 days before until 9 days after total hip arthroplasty (THA). Patients completed the Orthostatic Discriminant and Severity Scale five times during the study, to describe the patient burden potentially related to autonomic nervous system dysfunction.
Results
Standard deviation between normal-to-normal beats and the total power of HRV were reduced for at least nine days following THA, with a trend towards increased HRV leading up to the day of surgery. The balance between low- and high-frequency power of HRV was reduced in the postoperative evenings. There was increased orthostatic intolerance symptom burden on the first day after surgery, with symptoms of pain, fatigue, and weakness reduced in the postsurgical period. Median hospital stay was 1 day.
Conclusion
We provide the first detailed description of perioperative time-course of HRV and orthostatic symptoms in fast-track THA, showing reduced HRV after surgery for at least a week, and that HRV changes are sensitive to time of day and timing before and after surgery. These results are helpful in designing future HRV studies in perioperative risk assessment and outcome.
Reported reasons for non-use of insecticide-treated nets in large national household surveys, 2009-2020
Authors: Koenker Hannah , Kumoji E. Kuor , Erskine Marcy , Opoku Robert , Sternberg Eleanore , Taylor Cameron 
Published: October 2022

Abstract
Insecticide-treated nets (ITN) are the cornerstone of modern malaria vector control, with nearly 3 billion ITNs delivered to households in endemic areas since 2000. ITN access, i.e. availability within the household, based on the number of ITNs and number of household members, is a pre-requisite for ITN use. Factors determining ITN use are frequently examined in published literature, but to date, large household survey data on reasons given for non-use of nets have not been explored. A total of 156 DHS, MIS, and MICS surveys since 2003 were reviewed for questions on reasons why nets were not used the previous night, identifying twenty-four surveys. The percent of nets that were reported used the previous night was calculated for 156 surveys, and frequencies and proportions of reasons for non-use were calculated within the twenty-four surveys. Results were stratified by household supply of ITNs in three categories (not enough”, “enough”, and “more than enough”). The percent of nets used the previous night averaged 70.4% across the 156 surveys conducted since 2003. Reported reasons for non-use of ITNs were primarily nets being extra or being saved for later, followed by low perceived risk of malaria (no mosquitoes/no malaria). The least frequent categories cited as reasons for nets not being used were “net attributes” (size, shape, color, etc) and “fears”. In Senegal’s continuous DHS, the proportions of nets used peaked during high transmission season, while “no/few mosquitoes” responses peaked during the dry season. The proportion of nets used the previous night averaged over 70% from 2003–2020, with no discernible change over this period. Reported reasons for why a net goes unused fell largely into three categories - nets that are extra/being saved for future use; the perception that there is little risk of malaria (particularly in dry season); and “other” responses. Net attributes such as color, size, shape, and texture, and fears related to chemicals were the least frequent reasons given. Classifying reasons for non-use into broader categories facilitates the design of appropriate social and behaviour change interventions to address the major underlying reasons for non-use, where this is feasible.
Highly crystalline metal oxide semiconductor electron transport bilayers to improve the interface between the electron transport and active layer in planar perovskite solar cells
Authors: Lachore Wegene Lema , Andoshe Dinsefa Mensur , Mekonnen Mulualem Abebe , Tegegne Newayemedhin A. , Hone Fekadu Gashaw 
Published: October 2022

Abstract
In this study, we investigate the influence of a bilayer electron transport included of ZnO/SnO2, ZnO/WO3, and SnO2/WO3 on the interface between electron transport layers (ETLs) and perovskite layers to reduce energy loss in planar perovskite solar cells (P-PSCs). The structural, optical, and electrical properties, as well as the surface morphology, of perovskite thin film on different ETLs were investigated systematically by various characterization techniques. Impedance analysis of charge dynamics with PSC and without on metal oxide (MOs) were also studies. The X-ray diffraction (XRD) study confirmed that the prepared sample had a tetragonal rutile crystal structure for perovskite thin film on different ETLs. UV-visible spectra analysis revealed that perovskite thin films on various ETLs almost identical absorption onset at 752 nm were observed. The photoluminescence (PL) spectra study revealed that perovskite thin film on various ETLs an emissive band peaked at around 781 nm. The emission of the perovskite was quenched by 35.8%, for ZnO/MAPbI3 to 72.8% for ZnO/WO3/MAPbI3 to respectively. I–V studies confirmed that the prepared bilayer and singly layer had a good ohmic contact behavior and the resistivity decreased significantly from 128.87 Ω for WO3 to 112.49 Ω for ZnO/WO3 thin film respectively. These results revealed bilayer electron transport is more charge transfer property and charge collection, also there by suppressing trap-assisted recombination at the P-PSC interface. The overall results suggest that the bilayer of electron transport layers provides an efficient approach for improving the interface and fabricating efficient planar perovskite solar cells.