bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prelimbic Cortex Activity during a Distress Tolerance Task Predicts Cocaine-Seeking Behavior in Male, But Not Female Rats
Authors: Moschak Travis M. , Sloand T. Joseph , Carelli Regina M. 
Published: December 2022

Distress tolerance (DT) is defined as the ability to persist in challenging goal-directed behavior in the face of stress, and individuals with low DT exhibit heightened drug-seeking behavior. However, no preclinical studies have examined the neurobiology underlying this phenomenon. To assess this,in vivoelectrophysiology was used in Long Evans male and female rats during a DT task to record neural activity in the prelimbic cortex (PrL), a brain region implicated in drug-seeking. Rats were first assessed for DT, defined as the amount of time elapsed before rats quit seeking reward in an increasingly difficult operant task. Subsequently, rats underwent 2 weeks of self-administration for either water/saline or cocaine for 6 h/day. Animals then began a 1 month period of experimenter-imposed abstinence to induce heightened drug-seeking behavior. On day 28 of abstinence, DT and neural activity were reassessed; and on day 30, cocaine-seeking behavior was examined under extinction. Males had significantly higher DT than females and exhibited significantly more phasic PrL activity during the DT task. Furthermore, in male rats with a history of cocaine, PrL activity shifted to track DT; and this change in activity significantly correlated with the change in DT. Additionally, male (but not female) rats with low DT after 28 d of abstinence had significantly heightened drug-seeking behavior. Finally, PrL activity during the DT task predicted cocaine-seeking behavior. Collectively, these data demonstrate an important role for the PrL in DT in males, and link this neural activity and behavior to drug-seeking, particularly in males.SIGNIFICANCE STATEMENTDistress tolerance (DT) is defined as the ability to persist in challenging goal-directed behavior in the face of stress, and individuals with low DT exhibit heightened drug-seeking. Here, we investigated the role of the prelimbic cortex (PrL) in DT and its relationship to cocaine-seeking in male and female rats. We found that males had significantly higher DT than females and exhibited significantly more PrL activity during the DT task. Furthermore, male (but not female) rats with low DT after 28 d of abstinence had significantly heightened drug-seeking behavior. Finally, PrL activity during the DT task predicted cocaine-seeking. These data demonstrate an important role for the PrL in DT and link this neural activity and behavior to drug-seeking in males.
Cryo‐EM structures of human ABCA7 provide insights into its phospholipid translocation mechanisms
Authors: Le Le Thi My , Thompson James Robert , Dehghani‐Ghahnaviyeh Sepehr , Pant Shashank , Dang Phuoc Xuan , French Jarrod Bradley , Kanikeyo Takahisa , Tajkhorshid Emad , Alam Amer 
Published: December 2022

AbstractPhospholipid extrusion by ABC subfamily A (ABCA) exporters is central to cellular physiology, although the specifics of the underlying substrate interactions and transport mechanisms remain poorly resolved at the molecular level. Here we report cryo‐EM structures of lipid‐embedded human ABCA7 in an open state and in a nucleotide‐bound, closed state at resolutions between 3.6 and 4.0 Å. The former reveals an ordered patch of bilayer lipids traversing the transmembrane domain (TMD), while the latter reveals a lipid‐free, closed TMD with a small extracellular opening. These structures offer a structural framework for both substrate entry and exit from the ABCA7 TMD and highlight conserved rigid‐body motions that underlie the associated conformational transitions. Combined with functional analysis and molecular dynamics (MD) simulations, our data also shed light on lipid partitioning into the ABCA7 TMD and localized membrane perturbations that underlie ABCA7 function and have broader implications for other ABCA family transporters.
Kinerja Sistem Zero Water Discharge dengan Penambahan Probiotik dan Sumber Karbon pada Tahap Pendederan Ikan Bandeng (Chanos chanos)
Authors: Rahim Rahim , Husnaeni Husnaeni , Asni Asni , Hasirun Hasirun 
Published: December 2022

Penelitian ini bertujuan untuk mengetahui kinerja sistem zero water discharge melalui penambahan probiotik dan sumber karbon dari tepung tapioka pada tahap pendederan ikan bandeng (Chanos chanos) . Penelitian ini menggunakan dengan rancangan acak lengkap yang dengan tiga perlakuan dan tiga pengulangan. Perlakuan A : pendederan bandeng tanpa sistem ZWD, perlakuan B : pendederan bandeng sistem ZWD dengan penambahan probiotik, perlakuan C : pendederan bandeng ZWD dengan penambahan probiotik dan tepung tapioka. Hasil penelitian ini menunjukkan adanya perbedaan yang signifikan untuk setiap perlakuan (P<0,05) terhadap pertumbuhan dan kelangsungan nener ikan bandeng. Pertumbuhan dan kelangsungan hidup tertinggi dijumpai pada perlakuan C (zwd + probiotik + tepung tapioka) sebesar 0,11 g dan 75,56 %. Untuk parameter fisika-kimia air meliputi DO, suhu, pH salnitas, TAN dan nitrit masih berada pada kisaran yang baik untuk pendederan ikan bandeng.
Perbandingan Hasil Tangkapan Ikan Kakap Merah (Lutjanus malabaricus) pada Umpan yang Berbeda
Authors: Tri Hidayani Mesalina , Rapi Nuraeni L. , Murwantoko  , Djumanto  
Published: December 2022

Ikan kakap merah Lutjanus malabaricus merupakan jenis ikan dari family Lutjanidae yang memiliki daerah penyebaran cukup luas termasuk perairan Pinrang dan dapat tertangkap dengan berbagai jenis alat tangkap seperti rawai dasar, pancing ulur, dan bubu. Penelitian ini bertujuan untuk menganalisis hasil tangkapan kakap merah pada umpan yang berbeda sehingga upaya penangkapan kakap merah dapat lebih efektif dan efisien. Total 852 ekor kakap merah yang dikumpulkan secara acak dari alat tangkap rawai dasar, pancing ulur dan bubu di perairan Pinrang. Parameter yang diamati meliputi komposisi hasil tangkapan dan sebaran frekuensi panjang dengan menggunakan empat jenis umpan yaitu ikan teri, layang, tembang dan udang. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa hasil tangkapan kakap merah terbanyak diperoleh dari umpan udang sebanyak 305 ekor (36%) dan paling sedikit dari umpan ikan layang yaitu sebanyak 143 ekor (16%). Begitupula dengan sebaran ukuran panjang kakap merah yang berkisar antara 17-67 cm dengan frekuensi tertinggi sebanyak 89 ekor pada kisaran ukuran panjang 57-67 cm dari umpan udang dan terendah 9 ekor berada pada kisaran ukuran panjang 17-26 cm dari umpan ikan layang. Penggunaan umpan udang dapat meningkatkan hasil tangkapan Ikan kakap merah Lutjanus malabaricus.
Pengaruh Perbedaan Letak Muatan Secara Longitudinal Terhadap Gerak Oleng Model Kapal Pancing Ulur
Authors: Tandipuang Paduartama , Asia Asia , Nurwahidin Nurwahidin 
Published: December 2022

Setiap nelayan memiliki kebiasaan dan pertimbangan yang berbeda dalam hal menempatkan muatan di kapal ikan. Penempatan muatan yang tidak tepat sangat berbahaya karena dapat mengurangi stabilitas kapal. Diperlukan penelitian menggunakan skala model kapal untuk mengetahui letak muatan yang baik dan yang dapat membahayakan. Perlakuan yang diberikan meliputi M0 (kontrol, tanpa muatan), M1 (muatan di buritan), M2 (muatan di midship), dan M3 (muatan di haluan). Penelitian dilakukan untuk membandingkan jumlah frekuensi oleng, periode oleng, dan waktu henti oleng model kapal pada seluruh perlakuan. Percobaan dilakukan eksperimental di kolam terkontrol. Data dianalisa menggunakan ANOVA one way. Hasil analisa menunjukkan bahwa perlakuan letak muatan yang berbeda secara membujur memberikan pengaruh yang signifikan terhadap gerak oleng model kapal. Urutan rata-rata frekuensi oleng paling sedikit hingga paling banyak yaitu perlakuan M1 dan M2 (7,67 x), M3 (8 x), dan M0 (10 x). Urutan rata-rata periode oleng yang tercepat hingga yang terlama yaitu paerlakuan M2 (0,952 s), M0 (0,956 s), M1 (1,034 s), M3 (1,675 s). Urutan rata-rata waktu henti oleng tercepat hingga terlama yaitu perlakuan M2 (7,193 s), M1 (8,713 s), M0 (9,928 s), M3 (14,486 s). Perlakuan M3 memiliki periode oleng dan waktu henti oleng paling lama, sedangkan perlakuan M2 memiliki periode oleng dan waktu henti oleng paling cepat.
Pengaruh Kombinasi Estrak Labu Kuning (Cucubrbita mochata D.) dan Estrak Bunga Marigold (Tagetes sp.) pada Pakan terhadap Kecerahan Ikan Komet (Carassius auratus)
Authors: Nurhayati  , Diniarti Nanda , Dwi Hari Setyono Bagus 
Published: December 2022

Ikan Komet (Carrasius auratus) merupakan ikan hias air tawar dengan ciri khas keindahahan perpaduan warna oranye, putih dan merah. Tujuan penelitian ini adalah untuk menganalisa pengaruh kombinasi ekstrak labu kuning (Cucurbita moscahata D.) dan ekstrak bunga marigold (Tagetes sp.) pada pakan serta menyimpulkan dosis estrak terbaik untuk meningkatkan kecerahan warna ikan komet (Carrasius auratus). Metode yang digunakan adalah metode eksperimental terdiri dari 4 perlakuan dan 3 ulangan. Perlakuan tersebut terdiri atas A 0% (Kontrol), perlakuan B 50 ppm, perlakuan C 100 ppm, dan perlakuan D, 150 ppm% kombinasi ekstrak bunga marigold dan labu kuning. Hasil penelitian menunjukkan nilai kecerahan ikan komet berkisar 4,28-9,09, kandungan karotenoid sisik ikan 6,50 µmol/g- 9,44 µmol/g, karotenoid pakan 0,97µmol/g-1,2µmol/g, pertumbuhan berat mutlak 5,26 g-6,07g, pertumbuhan panjang mutlak 2,43-2,85 cm, pertumbuhan spesifik 2,46-2,72 %/hari, feed convertion ratio (FCR) 1,55-1,73, survival rate 97,8-100. Kisaran kualitas, suhu 27,1-29,6, DO 5,0-6,7 mg/L dan pH 7,1-8,0. Pemberian pakan dengan kombinasi ekstrak labu kuning dan ekstrak bunga marigold mampu memberikan warna pada ikan komet namun, tidak berpengaruh terhadap pertumbuhan yang optimum.
Pendugaan Thermal Front Sebagai Indikator Daerah Potensial Penangkapan Ikan Cakalang (Katsuwonus pelamis) di Laut Banda
Authors: Prasetya Arif , Hasidu La Ode Abdul Fajar , Maharani Maharani , Kasim Muh. , Mustasim Mustasim 
Published: December 2022

Daerah front merupakan salah satu proses oseanografi yang juga mempengaruhi kelimpahan dan penyebaran ikan. Penelitian ini bertujuan untuk mengidentifikasi beberapa parameter yang mempengaruhi distribusi ikan cakalang di Laut Banda, yaitu suhu permukaan laut (SPL) dan fenomena thermal front. Pengambilan sampel dilakukan pada bulan Februari hingga April dengan fishing base pelabuhan perikanan samudera (PPS) Kendari yang berlokasi di Laut Banda bagian barat dan fishing base bagian timur berada di pelabuhan perikanan Sorong. Data citra satelit menggunakan satelit aqua MODIS level 3 bulanan, analisis thermal front menggunakan metode cornillon dan cayula yang disempurnakan dalam tools single image edge detection (SIED) di aplikasi ArcGIS, uji regresi menggunakan R program yaitu Generalized Additive Model (GAM). Hasil identifikasi parameter oseanografi terhadap distribusi ikan cakalang: Analisis kemunculan thermal front didapatkan berpengaruh signifikan terhadap produksi ikan cakalang, dimana jarak ideal penangkapan ikan cakalang berkisar antara 0 – 18 km dari lokasi fishing ground, suhu permukaan laut berpengaruh signifikan terhadap produksi penangkapan ikan cakalang. Ikan cakalang di Laut Banda cenderung menempati ruang pada kisaran suhu permukaan laut (SPL) 28° – 30°C.
Inventarisasi dan Identifikasi Ektoparasit yang Menginfeksi Ikan Nila Hitam di P4S Karya Agri, Nusa Tenggara Timur
Authors: Sianturi Ion Tarsardo , Juanda Shobikhuliatul Jannah , Kamlasi Yusuf , Panuntun M. Fajar 
Published: December 2022

Pusat Pelatihan Pertanian dan Perdesaan Swadaya (P4S) Karya Agri merupakan salah satu tempat budidaya komoditi ikan air tawar yaitu ikan nila hitam (Oreochromis niloticus). Ikan ini banyak dibudidayakan di Kota Kupang dikarenakan kandungan gizi yang sangat tinggi sehingga permintaan pasar meningkat. Upaya dalam meningkatkan produksi ialah dengan cara menekan tingkat kegagalan yang salah satunya disebabkan oleh serangan parasit. Parasit merupakan mikroorganisme yang dapat menyerang ikan dan membuat daya tahan tubuh ikan menurun. Tujuan penelitian ini ialah untuk inventarisasi dan identifikasi ektoparasit yang menginfeksi serta melihat prevalensi dan tingkat intensitasnya. Metode deskriptif merupakan metode yang dipilih pada penelitian ini. Sampel ikan nila yang diamati memiliki panjang 14-15 cm. Pemeriksaan ektoparasit dilakukan dua cara yaitu pengamatan sirip dan insang yang dilihat dan diidentifikasi di bawah mikroskop. Pembesaran lensa mikroskop yang digunakan ialah 40x. Hasil penelitian memperlihatkan ektoparasit yang menginfeksi ikan nila hitam adalah Trichodina sp. dan Gyrodactylus sp. dengan intensitas rendah sedangkan nilai prevalensi yang didapat sebesar 5%.
Penilaian Sertifikat Kelayakan Pengolahan (SKP) Pada Unit Pengolahan Abon Ikan Tuna (Thunnus sp.)
Authors: Sayuti Mohammad , Salampessy Randi Bokhy Syuliana , Putra Rahmattullah Tio Buana 
Published: December 2022

Ikan tuna secara nasional merupakan komoditas ekspor terbesar kedua setelah udang. Salah satu pengolahan ikan tuna yang banyak dilakukan oleh kelompok masyarakat, baik industri rumah tangga maupun UMKM adalah industri abon. Tujuan penelitian ini untuk mengetahui kelayakan dasar penerapan GMP dan SSOP unit pengolahan abon ikan tuna dan mutunya pada UMKM Mamabon Kota Bukittinggi. Pengamatan proses pengolahan abon ikan dilakukan secara langsung di unit pengolahan menggunakan metode observasi dan wawancara dengan narasumber. Pengujian sensori bahan baku daging tetelan ikan tuna beku mengacu SNI 8919:2020 sedangkan sensori abon tuna mengacu pada SNI 7690:2013 yang dilakukan oleh 6 panelis dengan 8 kali pengamatan dan 3 kali pengulangan. Pengujian proksimat dan mikrobiologi dilakukan pada UPTD. Pengujian dan Penerapan Mutu Hasil Perikanan (PPMHP) Dinas Kelautan dan Perikanan Provinsi Sumatera Barat dengan dilakukan tiga kali pengulangan yang mengacu pada SNI. Perhitungan rendemen dilakukan pada tahap penyiangan, pengukusan, pencabikan dan produk akhir yang dilakukan sebanyak 5 kali pengamatan dengan 3 kali pengulangan. Penilaian Sertifikat Kelayakan Pengolahan (SKP) mengacu PermenKP No. 17 tahun 2019. Hasil uji sensori bahan baku dan produk abon tuna sudah memenuhi standar SNI. Abon ikan tuna memiliki nilai kadar protein 17,02%, kadar lemak 28,68%, kadar abu 4,92% dan kadar air 7,63%. Hasil pengujian mikrobiologi produk abon tuna sudah memenuhi standar SNI. Rendemen akhir abon ikan tuna sebesar 67%. Hasil penilaian SKP UMKM Mamabon mendapat predikat C (cukup). UMKM Mamabon telah menerapkan GMP dan SSOP walaupun dengan kategori cukup.
Muslim women adhering to Minangkabau’s bajapuik tradition in Cirebon, West Java: compromizing a gendered culture in Islamic law
Authors: Nuroniyah Wardah , Maula Bani Syarif 
Published: December 2022

Bajapuik refers to a customary engagement (khiṭbah) of the Minangkabau Pariaman community, where the prospective bride’s family gives a certain amount of money (japuik) to the groom’s family prior to a marriage. Islamic law allows a person to give a certain amount of money or gifts in the process of engagement. However, when the engagement is revoked and the marriage fails, the status of the money given during the engagement has been much contested. This study aims to explore and understand the positive relationship between Islamic law in the bajapuik tradition and local traditions. This study focuses on the legal aspects of engagement in the bajapuik tradition viewed from Islamic law and gender analysis. A descriptive qualitative approach was adopted with the primary data obtained through interviews. Data analysis was carried out using a legal approach, a sociological approach, and a philosophical approach. This study concludes that the bajapuik tradition depicts women as having a higher position than men. For the Pariaman community in Cirebon, the annulment of an engagement (khiṭbah) is a disgrace to tradition. An engagement is considered as a legally binding contract. In other words, customary provisions have coercive power to bounded communities. If an engagement is revoked without a valid reason, the responsible person can be sued for breaking the contract with certain compensations. Morevover, when a dispute occurs over property or finances, Minangkabau customary law allows the aggrieved party to take action against the khiṭbah violators in the form of returning property or money.
Innovation ecosystem as factor of providing progressive structural changes in the economy
Authors: Bazhal Iurii 
Published: December 2022

The article reveals issues of building the innovative ecosystem institutions problem in context of the growing role of research Universities in ensuring innovative structural changes in the economy of Ukraine. International comparisons of the actual state of effectiveness and development potential of Ukraine’s innovation ecosystem have shown that it lags behind almost all indicators of the European Innovation Scoreboard. It is a confirmed necessity to form in Ukraine a state innovation policy based on criteria of the European Innovation Scoreboard with the goal of improving all its indicators, which comprehensively reflect the state of the national innovation ecosystem.It has been focused on the formation of the central link of the innovation ecosystem – the entrepreneurial innovation University as a progressive structural change’s generator in the economy. The justified urgency of increasing the state support for the priority development of research, design and development, and educational institutions that are part of national and regional innovation ecosystems. It is necessary to radically increase the volume and change structure of the state funding of institutions in the field of research and development, as well as innovation activity, reducing the share of the state funding of individual projects and individual grants, which should be financed mainly by business structures.It is shown that the main practical problem in determining the place and role of research Universities and other scientific and educational institutions in the structural innovation transformation of the economy of Ukraine is insufficient attention to formation and implementation of the practical function of basic innovations as the main factor of economic growth. It is suggested under formation of the Ukrainian state innovation policy, a separate priority area related to the creation of higher education institutions (Universities) of new generation as innovative entrepreneurial Universities should be identified, while it is important to develop Universities and scientific centres that demonstrate the presence of cumulative human capital, despite their temporary state of crisis.
JEL classіfіcatіon: I23, I25, L52, O38, P52
Response of Row Ratio in Mustard (Brassica juncea L.) + Faba Bean (Vicia faba L.) Intercropping on Growth, Yield of Crops, System Productivity and Economics
Authors: Singh R.S. , Pandey I.B. , Choudhary C.S. , Kumar Amalendu , Narayan Ashish , Kumar Manoj 
Published: December 2022

Background: Mustard (Brassica juncea L.) as oilseeds and faba bean (Vicia faba L.) as pulses are very popular and potential crops but there is lack of research work on mustard + faba bean intercropping system. Therefore, this experiment was conducted to study the effect of mustard (Brassica juncea L.) + Faba bean (Vicia faba L.) intercropping system on growth, yield of crops, economics and system productivity. Methods: The study was carried out for three consecutive years during rabi seasons of 2015-16 to 2017-18 at the Research Farm of Tirhut College of Agriculture, Dholi under Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar. Treatments comprised of Sole mustard, Sole faba bean, Mustard+Faba bean (1:1), Mustard+Faba bean (1:2), Mustard+Faba bean (1:3), Mustard+Faba bean (1:4), Mustard+Faba bean (1:5), Mustard+Faba bean (2:4), Mustard+Faba bean (2:5) and Mustard+Faba bean (2:6) under replacement series. Result: This study finds that significantly higher number of branches/plant (5.0), number of pods/plant (30.9), number of grains/pod (2.21), 100-grain weight (28.94 g) of faba bean were recorded in 2:5 row ratio than sole faba bean, sole mustard, intercropping systems of faba bean with mustard in 1:1, 1:2 and 1:3 row ratios while plant height (134.4), number of branches/plant (10.93), number of pods/plant (170.6), number of grains/pod (9.95), 100-grain weight (0.49 g) of mustard were also found better in all the row ratios of mustard + Faba bean intercropping system than sole mustard. Maximum faba bean equivalent yield (3802 kg /ha) and net return (Rs. 92470/ha) were recorded under faba bean intercropped with mustard in 2:5 row ratio although it was found at par with mustard+faba bean in (1:5), (2:4) and (2:6) row ratios. Therefore, mustard and faba bean intercropping system in row ratio of 2:5 may be recommended.p
Hybrid Procedure in Aortoiliac Bifurcation and Femoral Lesion
Authors: Oktaviono Yudi Her 
Published: December 2022

Highlights:

A patient had a long segment of total occlusion to the proximal right common iliac and anterior tibial artery.
The treatment is a combination of percutaneous transluminal angioplasty (PTA) using vascular stent and the Fogarty thrombectomy.
Hybrid procedure can treat multilevel artery occlusive diseases, with shorter hospitalization, less perioperative morbidity, and similar early- and long-term efficacy.

Abstract:
Critical limb ischemia is a condition that threatens the viability of lower extremities and must be treated promptly to avoid major amputation. Revascularization is the most effective treatment method for critical limb ischemia. Revascularization using a hybrid of endovascular and open surgery is a minimally invasive procedure that performs well as the treatment for medically high-risk patients. A hybrid procedure should be considered for patients with high surgical risk. However, there are factors that could compromise its long-term patency, such as critical limb ischemia and diabetes. This study reported a case of a 53-year-old Asian male with history of insulin-dependent diabetes mellitus and long-standing tobacco use, presented with pain in the right leg at rest approximately 30 days prior to admission. Physical examination revealed a low temperature and remarkable non-palpable pulses in the right femoral, posterior tibial, and dorsalis pedis segments. Arteriography with run-offs revealed a long segment of total occlusion from the proximal right common iliac and anterior tibial artery. There was non-significant stenosis in the right popliteal artery. The patient was treated using a combination of percutaneous transluminal angioplasty (PTA) of the right common iliac artery using vascular stent and the Fogarty thrombectomy of the common femoral artery, equipped with an X-ray system and a moveable radiolucent surgical table. The anticoagulant used on the patient during the procedure was heparin. There was no residual stenosis after the procedure on the occlusion along the right common iliac artery to the common femoral artery. In conclusion, multilevel artery occlusive diseases could be treated by hybrid procedure, with shorter hospitalization, less perioperative morbidity, and similar early- and long-term efficacy compared with open revascularization.