bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing in vivo bioluminescence imaging and the Multi-Cruzi immunoassay platform to develop improved Chagas disease diagnostic procedures and biomarkers for monitoring parasitological cure
Authors: Francisco Amanda Fortes , Saade Ursula , Jayawardhana Shiromani , Pottel Hans , Scandale Ivan , Chatelain Eric , Liehl Peter , Kelly John M. , Zrein Maan 
Published: October 2022

Background
Chagas disease is caused by the protozoan parasite Trypanosoma cruzi and is a serious public health problem throughout Latin America. With 6 million people infected, there is a major international effort to develop new drugs. In the chronic phase of the disease, the parasite burden is extremely low, infections are highly focal at a tissue/organ level, and bloodstream parasites are only intermittently detectable. As a result, clinical trials are constrained by difficulties associated with determining parasitological cure. Even highly sensitive PCR methodologies can be unreliable, with a tendency to produce “false-cure” readouts. Improved diagnostic techniques and biomarkers for cure are therefore an important medical need.

Methodology/Principal findings
Using an experimental mouse model, we have combined a multiplex assay system and highly sensitive bioluminescence imaging to evaluate serological procedures for diagnosis of T. cruzi infections and confirmation of parasitological cure. We identified a set of three antigens that in the context of the multiplex serology system, provide a rapid, reactive and highly accurate read-out of both acute and chronic T. cruzi infection. In addition, we describe specific antibody responses where down-regulation can be correlated with benznidazole-mediated parasite reduction and others where upregulation is associated with persistent infection. One specific antibody (IBAG39) highly correlated with the bioluminescence flux and represents a promising therapy monitoring biomarker in mice.

Conclusions/Significance
Robust, high-throughput methodologies for monitoring the efficacy of anti-T. cruzi drug treatment are urgently required. Using our experimental systems, we have identified markers of infection or parasite reduction that merit assessing in a clinical setting for the longitudinal monitoring of drug-treated patients.
Every day mitral valve reconstruction: What has changed over the last 15 years?
Authors: Motazedian Farnoosh , Ostovar Roya , Hartrumpf Martin , Schröter Filip , Albes Johannes M. 
Published: October 2022

Objective
Mitral valve reconstruction (MVR) is one of the cardiosurgical procedures which cannot be substituted by any intervention owing to the quality of the quasi-anatomical, physiological repair. However, technique and strategies have changed over the years. We looked at procedural characteristics and outcome in an all-comer, non-selected cohort of patients.

Methods
738 out of 1.977 patients were retrospectively analyzed receiving MVR with and without concomitant procedures. The cohort was divided into three periods. P1: 2004–2009 (134 pts.); P2: 2010–2014 (294 pts.), and P3: 2015–2019 (310 pts.).

Results
Early mortality increased from P1 to P2 and decreased from P2 to P3 (9% P1, 13% P2, 10% P3). All patients received an annuloplasty-ring. In P1 resection measures dominated. In P3 artificial chordae were dominant. Age, BMI, and risk scores correlated with early mortality. Survival rates were 66% (5-years), 55% (10-years), 44% (15-years) in P1, 63% (5-years), 50% (10-years) in P2, and 80% (5-years) in P3. Odds ratio for reduced long-term survival were concomitant venous only bypass surgery (10-years 2,701, p = 0.026). 10-year survival was positively influenced by isolated MVR (0.246, p = 0.001), concomitant isolated arterial bypass (IMA) (0.153, p = 0.051), posterior leaflet measure (0.178, p<0.001), and use of artificial chordae (5-years 0.235, p<0.001).

Conclusion
Indication for ring implantation remained mandatory while preference changed alongside improved designs. Procedural characteristics changed from mainly resection maneuvers to predominant use of artificial chordae. Long-term results were negatively influenced by co-morbidities and positively influenced by posterior leaflet repair and artificial chordae. MVR underwent a qualitative evolution and remains a valuable cardiosurgical procedure.
Simulation models of sugary drink policies: A scoping review
Authors: Smith Natalie Riva , Grummon Anna H. , Ng Shu Wen , Wright Sarah Towner , Frerichs Leah 
Published: October 2022

Introduction
Simulation modeling methods are an increasingly common tool for projecting the potential health effects of policies to decrease sugar-sweetened beverage (SSB) intake. However, it remains unknown which SSB policies are understudied and how simulation modeling methods could be improved. To inform next steps, we conducted a scoping review to characterize the (1) policies considered and (2) major characteristics of SSB simulation models.

Methods
We systematically searched 7 electronic databases in 2020, updated in 2021. Two investigators independently screened articles to identify peer-reviewed research using simulation modeling to project the impact of SSB policies on health outcomes. One investigator extracted information about policies considered and key characteristics of models from the full text of included articles. Data were analyzed in 2021–22.

Results
Sixty-one articles were included. Of these, 50 simulated at least one tax policy, most often an ad valorem tax (e.g., 20% tax, n = 25) or volumetric tax (e.g., 1 cent-per-fluid-ounce tax, n = 23). Non-tax policies examined included bans on SSB purchases (n = 5), mandatory reformulation (n = 3), warning labels (n = 2), and portion size policies (n = 2). Policies were typically modeled in populations accounting for age and gender or sex attributes. Most studies focused on weight-related outcomes (n = 54), used cohort, lifetable, or microsimulation modeling methods (n = 34), conducted sensitivity or uncertainty analyses (n = 56), and included supplementary materials (n = 54). Few studies included stakeholders at any point in their process (n = 9) or provided replication code/data (n = 8).

Discussion
Most simulation modeling of SSB policies has focused on tax policies and has been limited in its exploration of heterogenous impacts across population groups. Future research would benefit from refined policy and implementation scenario specifications, thorough assessments of the equity impacts of policies using established methods, and standardized reporting to improve transparency and consistency.
Risk perception, community myth, and practices towards COVID-19 pandemic in Southeast Ethiopia: Community based crossectional study
Authors: Aliyi Ahmednur Adem , Ketaro Musa Kumbi , Engida Zinash Teferu , Argaw Ayele Mamo , Muhammed Abduljewad Hussen , Hassen Mesud Mohammed , Abduletif Abdushekur Mohammed , Shiferaw Damtow Solomon , Wodera Abate Lette , Ayene Sintayehu Hailu , Esmael Jeylan Kassim , Etu Edao Sinba 
Published: October 2022

Objective
The objective of this study was to assess risk perception, community myths, and preventive practice towards COVID-19 among community in Southeast Ethiopia, 2020.

Methods
Community-based cross-sectional study was conducted among 854 participants selected using a multistage sampling technique. Data were collected using a structured questionnaire adapted from previous literature. Descriptive statistics were done to summarize the variables. A generalized linear model with binary logistic specification was used to identify factors associated with risk perception and practice. Accordingly adjusted odds ratios with 95% confidence intervals were calculated and those with p-value < 0.05 were considered as significant factors associated with risk perception and practice. Cluster analysis using a linear mixed model was performed to identify factors associated with community myth and those with p-value <0.05 were reported as significant factors associated with community myth.

Results
All 854 respondents gave their answer yielding 100% response rate. Of these 547 (64.1%) were male, 611 (71.5%) were rural residents, 534 (62.5%) got information about COVID-19 from TV/radio, 591 (69.2%) of them live near health facility, 265 (30.8%) have a history of substance use and 100 (11.7%) have a history of chronic illness, and 415 (48.6%) of them have a high-risk perception, 428 (50.1%) have a wrong myth about COVID-19 and 366 (42.9%) have poor practice respectively. Residence, distances from health facility and myths were significantly associated with risk perception. Occupation, knowledge, and practice were significantly associated with community myths. Also level of education, living near health facilities, having good knowledge and wrong myth were significantly associated with the practice of utilizing COVID-19 preventive respectively.

Conclusion
The study found high-risk perception, high wrong community myth, and relatively low utilization of available practices towards COVID-19 and factors associated with them.
Do alexithymia and negative affect predict poor sleep quality? The moderating role of interoceptive sensibility
Authors: Huang Yun-Hsin , Yang Chien-Ming , Huang Ya-Chuan , Huang Yu-Ting , Yen Nai-Shing 
Published: October 2022

Objectives
Emotion-related hyperarousal is an important core pathology of poor sleep. Studies investigating the interplay of alexithymia and affective experiences in determining sleep quality have yielded mixed results. To disentangle the inconsistency, this study examined the concurrent predictive power of alexithymia, and negative and positive affect, while incorporating interoceptive sensibility (IS) as a possible moderator.

Methods
A sample of 224 (70.10% were female) participants completed the Toronto Alexithymia Scale, Positive and Negative Affect Schedule, Pittsburgh Sleep Quality Index, Multidimensional Assessment of Interoceptive Awareness (MAIA), and Marlowe‐Crowne Social Desirability Scale (for controlling response bias) using paper and pencil. A two-stage cluster analysis of the MAIA was used to capture IS characteristics. Stepwise regression was conducted separately for each IS cluster.

Results
A three-group structure for IS characteristics was found. Higher alexithymia was predictive of poor sleep quality in the low IS group, while higher negative affect predicted poor sleep quality in the moderate and high IS groups. Additionally, alexithymia and positive affect were significantly different in the three IS groups, while negative affect and sleep quality were not.

Conclusions
Emotion and cognitive arousal may impact sleep quality differently in individuals with different levels of internal focusing ability, depending on physiological versus emotional self-conceptualization. The implications on pathological research, clinical intervention, study limitations and future directions are discussed.
Trial protocol of an open label pilot study of lisdexamfetamine for the treatment of acute methamphetamine withdrawal
Authors: Acheson Liam S. , Ezard Nadine , Lintzeris Nicholas , Dunlop Adrian , Brett Jonathan , Rodgers Craig , Gill Anthony , Christmass Michael , McKetin Rebecca , Farrell Michael , Shoptaw Steve , Siefried Krista J. 
Published: October 2022

Introduction
Methamphetamine (MA) use disorder is an important public health concern. MA withdrawal is often the first step in ceasing or reducing use. There are no evidence-based withdrawal treatments, and no medication is approved for the treatment of MA withdrawal. Lisdexamfetamine (LDX) dimesilate, used in the treatment of attention deficit hyperactivity disorder and binge eating disorder has the potential as an agonist therapy to ameliorate withdrawal symptoms, and improve outcomes for patients.

Methods
A single arm, open-label pilot study to test the safety and feasibility of LDX for the treatment of MA withdrawal. Participants will be inpatients in a drug and alcohol withdrawal unit, and will receive a tapering dose of LDX over five days: 250mg LDX on Day 1, reducing by 50mg per day to 50mg on Day 5. Optional inpatient Days 6 and 7 will allow for participants to transition to ongoing treatment. Participants will be followed-up on Days 14, 21 and 28. All participants will also receive standard inpatient withdrawal care. The primary outcomes are safety (measured by adverse events, changes in vital signs, changes in suicidality and psychosis) and feasibility (the time taken to enrol the sample, proportion of screen / pre-screen failures). Secondary outcomes are acceptability (treatment satisfaction questionnaire, medication adherence, concomitant medications, qualitative interviews), retention to protocol (proportion retained to primary and secondary endpoints), changes in withdrawal symptoms (Amphetamine Withdrawal Questionnaire) and craving for MA (visual analogue scale), and sleep outcomes (continuous actigraphy and daily sleep diary).

Discussion
This is the first study to assess lisdexamfetamine for the treatment of acute MA withdrawal. If safe and feasible results will go to informing the development of multi-centre randomised controlled trials to determine the efficacy of the intervention.
Subjective and psychophysical olfactory and gustatory dysfunction among COVID-19 outpatients; short- and long-term results
Authors: Jensen Mads Mose , Larsen Kasper Daugaard , Homøe Anne-Sophie , Simonsen Anders Lykkemark , Arndal Elisabeth , Koch Anders , Samuelsen Grethe Badsberg , Nielsen Xiaohui Chen , Todsen Tobias , Homøe Preben 
Published: October 2022

Background
Olfactory and gustatory dysfunctions are early symptoms of SARS-CoV-2 infection. Between 20–80% of infected individuals report subjective altered sense of smell and/or taste during infection. Up to 2/3 of previously infected experience persistent olfactory and/or gustatory dysfunction after 6 months. The aim of this study was to examine subjective and psychophysical olfactory and gustatory function in non-hospitalized individuals with acute COVID-19 up to 6 months after infection.

Methods
Individuals aged 18-80-years with a positive SARS-CoV-2 PCR test no older than 10 days, were eligible. Only individuals able to visit the outpatient examination facilities were included. Gustatory function was tested with the Burgharts Taste Strips and olfactory function was examined with the Brief Smell Identifications test (Danish version). Subjective symptoms were examined through an online questionnaire at inclusion, day 30, 90 and 180 after inclusion.

Results
Fifty-eight SARS-CoV-2 positive and 56 negative controls were included. 58.6% (34/58) of SARS-CoV-2 positive individuals vs. 8.9% (5/56) of negative controls reported subjective olfactory dysfunction at inclusion. For gustatory dysfunction, 46.5% (27/58) of positive individuals reported impairment compared to 8.9% (5/56) of negative controls. In psychophysical tests, 75.9% (46/58) had olfactory dysfunction and 43.1% (25/58) had gustatory dysfunction among the SARS-CoV-2 positive individuals at inclusion. Compared to negative controls, SARS-CoV-2 infected had significantly reduced olfaction and gustation. Previously infected individuals continued to report lower subjective sense of smell 30 days after inclusion, whereafter the difference between the groups diminished. However, after 180 days, 20.7% (12/58) positive individuals still reported reduced sense of smell and taste.

Conclusion
Olfactory and gustatory dysfunctions are prevalent symptoms of SARS-CoV-2 infection, but there is inconsistency between subjective reporting and psychophysical test assessment of especially olfaction. Most individuals regain normal function after 30 days, but approximately 20% report persistent olfactory and gustatory dysfunction 6 months after infection.
Regularity of degenerate k-Hessian equations on closed Hermitian manifolds
Authors: Zhang Dekai 
Published: October 2022

Abstract
In this article, we are concerned with the existence of weak

C

1
,
1

{C}^{1,1}

solution of the

k

k

-Hessian equation on a closed Hermitian manifold under the optimal assumption of the function in the right-hand side of the equation. The key points are to show the weak

C

1
,
1

{C}^{1,1}

estimates. We prove a Cherrier-type inequality to obtain the

C

0

{C}^{0}

estimate, and the complex Hessian estimate is proved by using an auxiliary function, which was motivated by Hou et al. and Tosatti and Weinkove. Our result generalizes the Kähler case proved by Dinew et al.
Optimized linear regression control of DC motor under various disturbances
Authors: Gökçe Celal Onur 
Published: October 2022

AbstractIn this study, an optimized linear regression controller is proposed for velocity control of DC motor. System is tested under disturbances of different types. Step, sinusoidal and trapezoidal functions are used as reference input. A linear single layer network of weights is used to calculate the necessary armature voltage by giving past measured velocity, past reference and certain amount of future reference as inputs. Training data are generated using Proportional Integral (PI) controller parameters which are optimized using particle swarm optimization (PSO). In this first phase of training, pseudo-inverse solution is used to find the coarse parameters of the network. These parameters give suboptimal results with low performance even lower than that of PSO-optimized PI controller. In the second phase of training, parameters of the network are fine-tuned using PSO algorithm again, this time for optimization of network parameters. Quite encouraging results including up to more than 1,500% increase in performance are obtained and reported.
Late Cretaceous adakitic intrusive rocks in the Laimailang area, Gangdese batholith: Implications for the Neo-Tethyan Ocean subduction
Authors: Li Deliang , Mai Yuanjun , Yang Wenguang , Zhu Lidong 
Published: October 2022

Abstract
The Gangdese batholith, emplaced from the Cretaceous to the Eocene in the southern Lhasa terrane of Tibet, provides critical constraints on the tectonic-magmatic evolution of the Neo-Tethyan Ocean and the India-Asia continental collision. In this article, we report new data for the Laimailang monzogranite in the southern Gangdese, including major and trace element analyses, zircon U–Pb dating, and in situ Hf isotope analyses. In situ zircon U–Pb dating of sample yielded Late Cretaceous ages (ca. 81 Ma). The monzogranite is characterized by relatively high-silica (≥56 wt% SiO2), Na-rich, and high-Al granitoids that are characterized by high Sr, low Y and HREE contents and strongly fractionated REE patterns, with no significant Eu anomaly, indicating that they are consistent with the definition of adakite. These monzogranite have high K2O (2.92–6.5%) and negative ε
Hf(t) (−2.1 to −5.4), suggesting that these rocks were likely derived from the partial melting of the lower continental crust. We conclude that the Laimailang rocks of adakitic affinity were derived due to the melting of the thickened lower continental crust in the late Cretaceous.
COVID-19: a viewpoint from hepatic perspective
Authors: Brizawasi Abhijeet , Ahirwar Ashok Kumar , Prabhat  , Kaim Kirti , Ahirwar Pradeep , Kumawat Rajani , Prasad Jitender 
Published: October 2022

Abstract
Coronaviruses as such are known since last century. The name is derived from their shape which has crown (corona) like radiating spikes. The recent one however is a different one from the Coronavirus involved in SARS (2002–2004) and MERS (2012) in being highly infectious. Initially COVID 19 had a high case fatality rate which has now decreased to a significant extent. Many cases of COVID 19 are asymptomatic with a significant number of positive cases developing a triad of fever, breathlessness and GI symptoms. Recent travel increases the probability of infection. The pathogenesis involves ACE 2 receptors. So, it has been found that there are more cases and mortality among hypertensive individuals. Even higher among the people who use ACE inhibitor in comparison to those who use other anti-hypertensive drugs. Treatment is usually symptomatic. Antiviral drugs and vaccines against COVID-19 are being used. Deranged liver enzymes are common in COVID-19, however, serious liver injury is not much documented. Liver injury is either due to disease itself or due to antiviral drugs. Extra care like strict social distancing, avoiding unnecessary contact is needed for those with autoimmune hepatitis, liver cancer and those who are in immunosuppression because of a scheduled or already liver transplant. Further research is definitely needed in this field. The upcoming researches should also focus on liver injuries associated with disease course and derangements arising as side effects of treatment of COVID-19.
The smallest dislocated microduplication of Xq27.1 harboring SOX3 gene associated with XX male phenotype
Authors: Oroz Maja , Vičić Ana , Požgaj Šepec Marija , Karnaš Helena , Stipančić Gordana , Stipoljev Feodora 
Published: October 2022

Abstract

Objectives
Approximately 90% of “XX males” are positive for SRY. However, there are isolated cases of sex reversal associated to other genes in male-determining pathway.

Case presentation
We describe a 1.3-old patient with 46,XX karyotype, male phenotypic gender and cryptorchidism. Microarray analysis revealed a de novo 273 kb duplication in the Xq27.1 region that contains SOX3. FISH with probe specific to SOX3 confirmed a unique genomic location of this duplication, dislocated proximal to the centromere of the X chromosome.

Conclusions
This rare genetic condition was described in few other isolated cases that have associated SOX3 genetic rearrangements and DSD. Microarray and genome-wide-sequencing presents important part in routine diagnostics, and in delineation of other sex-determination-pathway genes in sex reversal disorders.
Severe consumptive hypothyroidism in hepatic hemangioendothelioma
Authors: Walther Antonia , Häberle Beate , Küppers Julia , Lurz Eberhard , Schmid Irene , Schmidt Heinrich , Dubinski Ilja 
Published: October 2022

Abstract

Objectives
Consumptive hypothyroidism may occur in hepatic hemangioendothelioma. The altered expression of deiodinases inactivates peripheral thyroid hormones. As a result, serum levels of free triiodothyronine and free thyroxine are reduced to varying degrees. There are no established recommendations for the dosage of sirolimus for this particular indication. We describe for the first time the course of treatment with low-dose sirolimus.

Case presentation
We present a 5-week-old infant with hepatic hemangioendothelioma and severe consumptive hypothyroidism. Due to hepatic infiltration he showed signs of right heart strain. Therapy of hemangioendothelioma was initiated with propranolol and, in the absence of response, methylprednisolone was added. Treatment was continued with low-dose sirolimus (due to side effects) and propranolol. Hypothyroidism was managed with levothyroxine and liothyronine.

Conclusions
Consumptive hypothyroidism due to cutaneous hemangioma and hepatic hemangioendothelioma can be managed with propranolol and low-dose sirolimus. Treatment of severe hypothyroidism may require a combinational therapy by substitution of both T3 and T4.
United States Policy in the Eastern Mediterranean
Authors: Yegin Mehmet 
Published: October 2022

AbstractThis article examines the United States’ approach to the Eastern Mediterranean. Despite reducing its military commitment and deciding to pursue a policy less inclined to use military force, the US has neither withdrawn its military presence completely nor abandoned its allies in the region. Instead, it has recalibrated its involvement, limiting its engagement and related costs to reallocate resources to the Asia Pacific. However, the US still seeks to prevent Russia and China from co-opting its partners in the Eastern Mediterranean, just as it strives to combat terrorism there. So, Washington seeks to maintain the status quo provided by the current balance of its regional allies and to prevent Russia and China from establishing strategic relations with one or more of those regional actors. However, the US track record in achieving those objectives is mixed, especially when comparing the performances of different administrations.