bluuebunny/crossref_metadata_2025_split huggingface dataset:
Which one? A comparative study of traditional and sports uniforms on academic achievement, cognitive performance, playtime, bullying, and discrimination in adolescents. The Cogni-Action Project
Authors: Cristi-Montero Carlos , Solis-Urra Patricio , Sanchez-Martinez Javier , Olivares-Arancibia Jorge , Hernández-Jaña Sam , Gajardo-Araya Guillermo , Palma-Leal Ximena , Sadarangani Kabir P. , Estinto Matias Portela , Encina Yonatan , Alvarez Cristian , Delgado-Floody Pedro , Aguilar-Farias Nicolas , Ferrari Gerson , Mahecha-Matsudo Sandra , Zavala-Crichton Juan Pablo , Ibarra-Mora Jessica , Parra-Saldías Maribel , Nanjarí-Miranda Rodrigo , Rodríguez-Rodríguez Fernando 
Published: April 2022

Abstract

Objective
The aim of the study was to compare academic achievement, cognitive performance, playtime, bullying, and discrimination in adolescents according to traditional (TU) and sports uniforms (SU) worn at school, while simultaneously exploring the influence of the school vulnerability index.
Methods
Nine hundred eighty-eight Chilean adolescents (52.6% boys) aged 10–14 years participated in this cross-sectional study. Academic achievement was evaluated by the average grade in maths, language, and science grades, while the cognitive performance was assessed through eight cognitive tasks. TU affecting physical activity, playtime, bullying, and discrimination were queried. Mixed model analyses were performed.
Results
No differences were observed in academic achievement (TU: 5.4 ± 0.1 vs. SU: 5.5 ± 0.2, p-value = 0.785) or in cognitive performance (TU: 99.6 ± 0.8 vs. SU: 98.9 ± 1.8, p-value = 0.754) according to school uniform type. Moreover, 64.1% of participants declared that wearing TU affects their physical activity, and those who believed so spent more time playing than those who answered negatively (14.5 min, p-value = 0.012). Finally, adolescents wearing SU displayed a lower feeling of bullying and discrimination; this finding depended mainly on the school’s vulnerability.
Conclusion
It is concluded that wearing a TU does not show an educational advantage at an academic and cognitive level that justifies its obligation. In addition, it could be suggested that schools consider adolescents’ opinions in adopting a more comfortable uniform, such as the SU. This feasible and low-cost measure would help to increase adolescents' physical activity during the school day and, contrary to belief, it would not be related to increased feelings of bullying and discrimination.
The Role of N6-Methyladenosine-associated lncRNAs in Immune Microenvironment and Prognosis of Colorectal Cancer
Authors: Yuan Congfei , Liu Caidong , Zhao Shuli , Zhang Xishan , Jia Haifeng , Chen Baiyu , Zhang Maojin , Zheng Yuan , Zhou Jin , Bo Yanzhi 
Published: April 2022

Abstract
Background
The role of N6-methyladenosine long non-coding RNAs (lncRNAs) in colorectal cancer (CRC) is elusive.
Materials and Methods
We identified m6A-associated lncRNAs by using the data gathered from The Cancer Genome Atlas (TCGA) and stratified CRC patients into different subgroups. Cox-regression analysis were performed to construct a m6A-associated lncRNA signature. The role of this signature in immune microenvironment and prognosis was dissected subsequently. Finally, a gene set enrichment analysis (GSEA) was conducted to predict the possible mechanisms based on the signature.
Results
Three m6A-associated clusters were constructed from 866 differentially expressed lncRNAs. Cluster 2 had poor prognosis and low immune cell infiltration. A m6A-associated lncRNA signature consisting of 14 lncRNAs was constructed, and recognized as an independent prognostic indicator of CRC by using survival analysis and receiver operating characteristic (ROC) curves. The clinical features and immune cell infiltration status were significantly different in patients stratified by risk score. Furthermore, GSEA showed that P53 pathway and Natural killer cell mediated cytotoxicity were more enriched in the low-risk group.
Conclusion
Our data revealed that m6A-associated lncRNAs could be potential prognostic indicator of immunogenicity in CRC.
Effects of high-protein feed supplements on lamb productivity
Authors: Marynich Aleksandr , Abilov Batyrkhan , Semenov Vladimir , Dzhafarov Novruz , Kulintsev Valery , Serdyukov Igor 
Published: April 2022

Introduction. Today’s feed market offers a variety of new products of plant and animal origin that increases the productivity of young sheep. Using feed supplements can help farmers to fully realize the genetic potential of wool-and-meat genotype sheep.
Study objects and methods. We studied the effect of a whole milk replacer (skimmed powdered milk) and an ORGANIC high-protein feed supplement on the growth of young sheep and the quality of their meat. In particular, we determined the effect of starter feeds on the biochemical and morphological parameters of sheep blood at the Vtoraya Pyatiletka Breeding Farm, Stavropol Krai.
Results and discussion. Substituting starter feeds with a whole milk replacer and an ORGANIC supplement for the standard feed in the diet of sheep aged 0–4 months increased metabolic energy (by 12.5%), crude protein (by 22.4 and 25.5%, respectively), lysine (by 24.8 and 21.4%, respectively), and methionine + cystine (by 31.0%). The starter feeds also led to higher live weight (by 29.6 and 33.7% (P ≤ 0.001)), absolute and average daily gain (by 24.6 and 29.1% (P ≤ 0.001)), slaughter weight (by 36.5 and 42.1% (P ≤ 0.001)), slaughter yield (by 2.50 and 2.96 abs.% (P ≤ 0.05)), and meat marbling (by 3.6 and 11.7%). The number of muscle fibers increased by 2.1 and 3.3%, respectively. Additional profits rose from 1761.5 to 2091.5 rubles per head and the product profitability reached 50.5–57.9%.
Conclusion. The starter feeds containing a milk replacer and an ORGANIC feed supplement proved effective for sheep aged of 0–4 months in the suckling period, ensuring live weight of 39–40 kg and improving meat quality and productivity.
Pharmacology and Ethnomedicinal Potential of Selected Plants Species from Apiaceae (Umbelliferae)
Authors: Patra J.K. , Das G. , Das S. , Talukdar A.D. , Venil C.K. , Bose S. , Banerjee S. , Shin H.-S. , Gutiérrez-Grijalva E.P. , Heredia J.B. 
Published: April 2022

Background:The Apiaceae or Umbelliferae is one of the largest families in terms of species representation in the plant kingdom. It is also a prominent family in the field of phytochemicals and pharmacology. The family is also quite prominent in the production of spices and condiments and food supplements in nutrition, aside from the potential of species in the family to induce apoptotic, antimicrobial, antitumor, and hepatoprotective activities.Objective:This work presents a detailed structural elucidation and functional aspects of phytochemicals from the Apiaceae or Umbelliferae family.Method:Furthermore, the application of members of this family in traditional and modern pharmacology is emphasized. This review also highlights the linkage of phytochemicals used in the conventional system of medication for the development of novel therapeutics through a chain of pre-clinical and clinical trials.Conclusion:This study may represent a valuable step ahead in the clinical development of natural drugs for curing several ailments, including respiratory and virus-related diseases.
ADAM19 and TUBB1 Correlate with Tumor Infiltrating Immune Cells
and Predicts Prognosis in Osteosarcoma
Authors: Xing Deguo , Wang Jun , Gong Mingzhi , Xiong Zhenggang , Zhao Yangyang 
Published: April 2022

Background:
Osteosarcoma is the most common type of primary malignant bone tumor.

Introduction:
This study aimed to explore potential key prognostic genes and their roles in osteosarcoma.

Methods:
Three microarray datasets for osteosarcoma were downloaded from the GEO database.
Differentially expressed genes (DEGs) were screened by the Limma package. Functional enrichment
analysis was performed based on DAVID, GeneMANIA, and Metascape databases. Prognostic
value of DEGs was elevated by survival analysis. CIBERSORT was used to assess the infiltrating
abundance of 22 immune cells, followed by the Pearson correlation analysis between immune
cells and prognosis-related genes. Gene set enrichment analysis and drug-gene interactions prediction
were performed for prognosis-related genes.

Results:
A total of 8 common up-regulated DEGs and 13 common down-regulated DEGs were
screened in the GSE36001 and GSE56001 datasets. Enrichment analysis showed these DEGs were
implicated in platelet activation, SMAD protein phosphorylation, lymphocyte/leukocyte/T cells activation,
and cell migration. Survival analysis indicated that elevated expression of ADAM19 and
TUBB1 were associated with a favorable prognosis. CIBERSORT algorithm revealed the higher
infiltrating level of CD8 T cells, macrophages M0, and M2 in osteosarcoma. ADAM19 expression
positively correlated with naïve B cells and negatively correlated with activated dendritic cells infiltrating
abundance. TUBB1 expression positively correlated with gamma delta T cells while negatively
correlated with helper follicular T cells infiltrating abundance. A total of 56 drugs were
found to target TUBB1.

Conclusion:
ADAM19 and TUBB1 could be prognostic biomarkers in osteosarcoma. Both their
expression correlates with tumor infiltrating immune cells. TUBB1 was a multi-drug target that
might be a therapeutic target in osteosarcoma.
Research progress of human AP endonuclease 1: structure, catalytic mechanism and inhibitors
Authors: Zhang Zelan , Wu Zhixiang , Shi Xiaodong , Guo Du , Cheng Yan , Gao Jiaxing , Liu Ling , Liu Wei , Liang Li , Peng Lianxin , Hu Jianping 
Published: April 2022

Abstract:
The high stability of phosphodiester bonds is considered to be one of the important reasons for the genetic role of nucleic acids, and their cleavage is also the core of many key biochemical processes, including DNA replication / repair, and RNA processing / degradation. As an important part of the base excision repair (BER) pathway, human apurinic / apyrimidinic endonuclease 1 (APE1) is indispensable for the repair of a basic sites and other DNA damage including ionizing radiation, DNA covalently bonding induced by cytotoxic antitumor drugs, etc. To tumor cells, the DNA repair activity of APE1 may lead to the occurrence of radiotherapy and chemotherapy resistance. Obviously, the overexpression of APE1 often poses a serious threat to the effectiveness of tumor treatment, indicating longer time, much larger dose, less effective chemotherapy and poor prognosis. It is of great urgency to design novel APE1 inhibitors. Rational design and modification of inhibitor molecules are closely related to the research progress of both structural biology and catalytic mechanism. In this review, the structure, catalytic mechanism, inhibitors and other important biochemical information of APE1 all are summarized, which will help for the design and modification of drug molecules targeting APE1.
Inhibitor of Growth 4 (ING4) Plays a Tumor-repressing Role in Oral
Squamous Cell Carcinoma via Nuclear Factor Kappa-B (NF-kB)/DNA Methyltransferase
1 (DNMT1) Axis-mediated Regulation of Aldehyde Dehydrogenase
1A2 (ALDH1A2)
Authors: Cui Zhi , Sun Shiqun , Li Jia , Li Jianing , Sha Tong , He Jie , Zuo Linjing 
Published: April 2022

Background:
Inhibitor of growth 4 (ING4) level was reported to be decreased in head and
neck squamous cell carcinoma (HNSC) tissue, however, it is unknown whether and how ING4 participates
in regulating the development of oral squamous cell carcinoma (OSCC).

Objective:
This study aimed to investigate the role and mechanism of ING4 in OSCC.

Methods:
ING4 was forced to up- or down-regulated in two OSCC cell lines, and its effects on the malignant
behavior of OSCC cells were investigated in vitro. The ubiquitination level of NF-kB p65 in
ING4 upregulated cells was measured by co-immunoprecipitation. Moreover, the effects of ING4 on
the methylation level of ALDH1A2 were evaluated by methylation-specific polymerase chain reaction
(MSP) assay. The role of ING4 in OSCC growth in vivo was observed in nude mice.

Results:
Our results showed that the expression of ING4 in OSCC cell lines was lower than that in
normal oral keratinocyte cells. In vitro, ING4 overexpression inhibited the proliferation, migration, and
invasion of OSCC cell lines and ING4 silencing exhibited opposite results. We also demonstrated that
ING4 overexpression promoted the ubiquitination and degradation of P65 and reduced DNA methyltransferase
1 (DNMT1) expression and Aldehyde dehydrogenase 1A2 (ALDH1A2) methylation. Moreover,
overexpression of p65 rescued the suppression of malignant behavior, induced by ING4 overexpression.
In addition, ING4 negatively regulated the growth of OSCC xenograft tumors in vivo.

Conclusion:
Our data evidenced that ING4 played a tumor-repressing role in OSCC in vivo and in vitro
via NF-κB/DNMT1/ALDH1A2 axis.
Rethinking Catatonia: New Insights from the Autism Spectrum
Authors: Amatori Giulia , Dell’Osso Liliana , Toschi Daniela , Gesi Camilla 
Published: April 2022

Abstract:
Initially conceptualized as a subtype of schizophrenia, catatonia has progressively been recognized
to occur in a wide variety of conditions as a kind of final common pathway for many severe
mental disorders. There is a significant overlap between the fundamental symptoms of autism spectrum
disorder (ASD) and catatonia, and ASD can frequently complicate catatonic states. While
provocative, the hypothesis that neuroatypicality may represent the submerged structural piece underlying
catatonic states is intriguing. Yet, the multifaced relationship between catatonia and ASD may
provide new insights into the etiology and treatment of both disorders.
Notopterygium incisum Root Extract (NRE) Alleviates Epileptiform Symptoms in PTZ-Induced Acute Seizure Mice
Authors: Jiang Xiaowen , Zhao Qingchun , Zhou Lijun , Gao Yaping , Lu Hongyuan , Liu Wenwu , Xu Xiangbo , Xing Bo , Liang Xu , Wang Nan 
Published: April 2022

Background:Epilepsy is a common neurological disorder affecting more than 70 million people worldwide. Despite numerous efforts on new antiepileptic drugs, approximately one-third of epilepsy patients suffer from uncontrolled seizures. It leads to serious psychosocial consequences, cognitive problems, and decreased quality of life.Objective:Our previous studies have shown that N. incisum root extract (NRE) can improve cognitive dysfunction in Alzheimer's disease (AD) mice. In addition, our research shows that AD and epilepsy have pathological mechanisms overlapping. Therefore, we tried to investigate whether NRE can ameliorate the seizures of epileptic mice in this study.Methods:NRE-treated mice group was given an oral administration with 1 g/kg/d for 7 days. On the 8th day, mice were exposed to PTZ (i.p. injection) to induce epilepsy. Then the cognitive tests of mice in the water maze were carried out, and the biochemical indexes and pathological tests were carried out after the mice were sacrificed.Results:SOD level in the NRE group was significantly higher than that in the PTZ group, while MDA, TNF-α, and IL-1β levels were decreased. The cognitive ability of NRE-treated mice was significantly improved compared with the PTZ group. Immunohistochemistry (IHC) results showed that the activation of microglia and astrocytes in the hippocampus and cortex of NRE mice were inhibited.Conclutions:This study suggests that NRE can alleviate epilepsy and improve cognitive function in mice with epilepsy, and its mechanism may be through reducing inflammation and enhancing antioxidant defense.
The Screening Program for Autism Spectrum Disorders in the West of Iran
Authors: Bashirian Saeid , Jenabi Ensiyeh , Khazaei Salman , Zareian Sepideh , Hamzehei Ronak , Razjouyan Katayoon , Zarafshan Hadi , Seyedi Mahdieh , Nouri Shahla , Rezaei Mohammad , Pourghavam Zahra Moradi , Babakhani Narges 
Published: April 2022

Background:
There are few epidemiological data regarding the autism spectrum
disorders (ASD) screening program in Iran, and as yet, screening for children at the age of
18 months has not been performed.

Aim:
The present study aimed to perform a screening program for children with ASD in
Iran.

Method:
This cross-sectional study was conducted for screening children with ASD. The
age and stages Questionnaire-18 (ASQ-18) month questionnaire was sent to all parents of
children via a mobile-based application.The Modified Checklist for Autism in Toddlers (MCHAT)
questionnaire was mailed if the child's score was below the cutoff. For those with
positive M-CHATs, the M-CHAT/F Follow-up interview was performed. Finally, children
with ASD, according to The Autism Diagnostic Interview-Revised (ADI-R) and examination,
were diagnosed.

Result:
Of these children, 1221 (48%) were girls and 1341 (52%) were boys. Of the parent
respondents, 232 (9%) children had a positive ASQ-18 questionnaire. For this purpose, the MCHAT
was sent to the parents of these children. Of the 232 children with ASQ positive, 179
(77.1%) parents completed M-CHAT. On the M-CHAT, 43 (24.0%) children screened positive
and 136 (76.0%) children passed on M-CHAT-R screening. In this stage of the 43 screenpositive
children, health care providers asked parents to refer to their children for performing
the M-CHAT at follow up. For a total of 33 children, the M-CHAT follow-up was performed.
Of these samples, 7 children had positive M-CHAT follow-up. Therefore, ADI-R was conducted
for these children to diagnose ASD. In the end, based on ADI-R and diagnostic evaluation
performed by the child and adolescent psychologists, 7 children had ASD.

Conclusion:
The findings of the present study show that a mobile-based application can be
utilized for screening children at risk of ASD. Therefore, we suggest that the application be
used for screening children with ASD.
Programs Using Stimulation-Regulating Technologies to Promote Physical Activity in People With Intellectual and Multiple Disabilities: Scoping Review
Authors: Lancioni Giulio E , Singh Nirbhay N , O’Reilly Mark , Sigafoos Jeff , Alberti Gloria , Desideri Lorenzo 
Published: April 2022

Background
People with intellectual and multiple disabilities tend to engage in very low levels of physical activity.

Objective
This review paper aims to provide a comprehensive picture of intervention programs using stimulation-regulating technologies to promote forms of physical activity in people with intellectual and multiple disabilities.

Methods
Following the PRISMA-ScR (Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews) checklist, a scoping review was conducted to identify and provide a synthesis of eligible studies published in English between 2010 and 2021. Studies were identified by searching PubMed, Web of Science, PsycINFO, ERIC, and CINAHL as well as by using Google Scholar and manual searches. Studies were included if they involved individuals with intellectual or multiple disabilities, used stimulation-regulating technology systems to help participants engage in physical activity, and reported data on the impact of the intervention.

Results
A total of 42 studies met the inclusion criteria. These studies were divided into 2 groups based on whether they pursued the increase in physical activity through technology-aided delivery of brief periods of preferred stimulation contingent on specific responses or the use of video games (exergames) and related auditory and visual stimulation. Subsequently, a narrative synthesis of the studies was provided.

Conclusions
The evidence reported by the 2 groups of studies is encouraging. However, further research is needed to compare the overall applicability and impact of the intervention strategies proposed by these groups of studies.
The Effectiveness of Serious Games on Cognitive Processing Speed Among Older Adults With Cognitive Impairment: Systematic Review and Meta-analysis
Authors: Abd-alrazaq Alaa , Ahmed Arfan , Alali Haitham , Aldardour Ahmad Mohammad , Househ Mowafa 
Published: April 2022

Background
Human cognitive processing speed is known to decline with age. Human cognitive processing speed refers to the time that an individual takes from receiving a stimulus to reacting to it. Serious games, which are video games used for training and educational purposes, have the potential to improve processing speed. Numerous systematic reviews have summarized the evidence regarding the effectiveness of serious games in improving processing speed, but they are undermined by some limitations.

Objective
This study aimed to examine the effectiveness of serious games on the cognitive processing speed of an older adult population living with cognitive impairment.

Methods
A systematic review of randomized controlled trials (RCTs) was conducted. Two search sources were used in this review: 8 electronic databases and backward and forward reference list checking. A total of 2 reviewers independently checked the eligibility of the studies, extracted data from the included studies, and appraised the risk of bias and quality of the evidence. Evidence from the included studies was synthesized using a narrative and statistical approach (ie, meta-analysis), as appropriate.

Results
Of the 548 publications identified, 16 (2.9%) RCTs eventually met all eligibility criteria. Very-low-quality evidence from 50% (8/16) and 38% (6/16) of the RCTs showed no statistically significant effect of serious games on processing speed compared with no or passive intervention groups (P=.77) and conventional exercises (P=.58), respectively. A subgroup analysis showed that both types of serious games (cognitive training games: P=.26; exergames: P=.88) were as effective as conventional exercises in improving processing speed.

Conclusions
There is no superiority of serious games over no or passive interventions and conventional exercises in improving processing speed among older adults with cognitive impairment. However, our findings remain inconclusive because of the low quality of the evidence, the small sample size in most of the included studies, and the paucity of studies included in the meta-analyses. Therefore, until more robust evidence is published, serious games should be offered or used as an adjunct to existing interventions. Further trials should be undertaken to investigate the effect of serious games that specifically target processing speed rather than cognitive abilities in general.

Trial Registration
PROSPERO CRD42022301667; https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=301667