bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative Imaging of Costal Cartilage to Aid Reconstructive Head and Neck Surgery
Authors: Faderani Ryan , Arumugam Vinodh , Tarassoli Sam , Jovic Thomas H , Whitaker Iain S 
Published: November 2022

Objective
Autologous costal cartilage is used extensively in reconstructive surgery because of its stability, durability, and biocompatibility. The current preoperative evaluation of costal cartilage often only consists of a physical examination. Several studies have highlighted the benefits of preoperative imaging as a tool to ensure optimal graft harvest. This systematic review aims to synthesize the current evidence and establish the efficacy of the various imaging modalities for the assessment of costal cartilage.

Review Methods
The data sources were explored using a search strategy based on the terms (“costal cartilage” OR “ribs” AND “imaging*”) combined with Boolean operators. The primary outcome measures were the ability to measure the dimensions of costal cartilages and to detect the presence of calcifications.

Results
A total of 28 publications were included in the final review, with 12 case series, 7 case control studies, and 9 cohort studies. Twenty-two studies used computed tomography (CT); 4 studies used x-ray and 2 studies used ultrasonography, whereas no studies used magnetic resonance imaging. Meta-analysis of the data from these studies was not deemed possible.

Conclusions
Our findings suggest that CT is the modality with the strongest evidence base that provides the greatest degree of information. The major benefits of CT are its ability to provide 3-dimensional image reconstruction for surgical planning, ability to detect synchondroses, and assess cartilage quality. Where radiation exposure is less preferable, x-ray and ultrasound (US) may play an important role. X-ray appears to be particularly useful when the main concern is the presence of calcification. The limited studies available indicate that US can provide useful and accurate information on cartilage quality and morphology. Further studies are warranted in exploring the use of US in preoperative planning, particularly in the pediatric population.
Structure of the peroxisomal Pex1/Pex6 ATPase complex bound to a substrate
Authors: Rüttermann Maximilian , Koci Michelle , Lill Pascal , Klink Björn Udo , Erdmann Ralf , Gatsogiannis Christos 
Published: November 2022

AbstractThe double-ring AAA+ ATPase Pex1/Pex6 is required for peroxisomal receptor recycling and is essential for peroxisome formation. Pex1/Pex6 mutations cause severe peroxisome associated developmental disorders. Despite its pathophysiological importance, mechanistic details of the heterohexamer are not yet available. Here, we report cryoEM structures of Pex1/Pex6 fromSaccharomyces cerevisiae, with an endogenous protein substrate trapped in the central pore of the second ring (D2). Pairs of Pex1/Pex6(D2) subdomains engage the substrate via a staircase of pore-1 loops with distinct properties. The first ring (D1) is catalytically inactive but undergoes significant conformational changes resulting in alternate widening and narrowing of its pore. These events are fueled by ATP hydrolysis in the D2 ring and disengagement of a “twin-seam” Pex1/Pex6(D2) heterodimer from the staircase. Mechanical forces are propagated in a unique manner along Pex1/Pex6 interfaces that are not available in homo-oligomeric AAA-ATPases. Our structural analysis reveals the mechanisms of how Pex1 and Pex6 coordinate to achieve substrate translocation.
Chronic broadband noise increases the fitness of a freshwater zooplankton
Authors: Prosnier Loïc , Rojas Emilie , Valéro Olivier , Médoc Vincent 
Published: November 2022

AbstractAlthough there is an increasing interest in the effects of anthropogenic noise on underwater wildlife, most studies focus on marine mammals and fishes while many others taxa of substantial ecological importance are still overlooked. This is the case for zooplankton species which ensure the coupling between primary producers and fishes in pelagic food webs. Here, we measured lifespan, reproduction and mobility of laboratory-raised water fleasDaphnia magna, a widespread freshwater zooplankton species, in response to continuous broadband noise. Surprisingly, we found a significant increase in survival and fecundity, leading to a higher fitness when considering total offspring production and a slight increase in population growth rate, according to the Euler-Lotka equation. Exposed water fleas were found slower than control individuals and we hypothesised that the energy saved from reduced mobility was reallocated to survival and fecundity. Our results can have implications in aquaculture where the acoustic environment receives little attention. Chronic broadband noise can be associated with certain human activities but the consequences on naturalDaphniapopulations might differ as reduced velocity could have negative outcomes when considering competition and predation. Our result highlights for the first time that, despite the absence of a known hearing system, a zooplanktonic crustacean is affected by chronic noise.
One-day preoperative systemic treatment regimen outcompetes five-day regimen in potentially resectable esophageal squamous cancer
Authors: Wang Sichao , Huang Shujie , Gao Zhen , Tang Yong , Zhou Zihao , Qiao Guibin 
Published: November 2022

AbstractObjectiveThe current study aimed to compare the effectiveness of the one-day immunochemotherapy regimen, the one-day chemotherapy regimen and the five-day chemotherapy regimen in locally advanced esophageal squamous cell carcinoma patients treated with preoperative systemic treatment.MethodsWe retrospectively analyzed locally advanced ESCC patients who had received POST from January 2012 to September 2021 at the Department of Thoracic Surgery, Guangdong Provincial Peoples’ Hospital. The clinical and follow-up data were collected and analyzed according to 3 regimens. Categorical and continuous variables were analyzed by Chi-square and One-way ANOVA respectively, and overall survival (OS) was assessed using Kaplan-Meier analysis.ResultsA total of 395 POST-treated ESCC patients were enrolled, including 72 in the 5FU-based group, 168 in the pla/pac group, and 155 in the pla/pac/ICI group, and the mean follow-up time were 32.2, 44.2 and 14.3 months, respectively. the pla/pac/ICI group had the greatest benefit, with an ORR of 63.2% (P < 0.05) and a surgery conversion rate of 85.2% (P < 0.05). Furthermore, pla/pac/ICI group acquired a better short-term OS than the other groups (one-year OS: pla/pac/ICI 93.6% vs. pla/pac 87.4% vs. 5FU-based 70.5%).ConclusionIn the context of the COVID-19 pandemic, one-day immunochemotherapy should be considered because it may yield higher response rates, bring better overall survival as well as greatly reduce the risk of treatment interruption. If immunotherapy is not available, the 1-day pla/pac regimen is also an effective and timely alternative.
Antibody binding reports spatial heterogeneities in cell membrane organization
Authors: Arnold Daniel P. , Xu Yaxin , Takatori Sho C. 
Published: November 2022

AbstractThe spatial organization of cell membrane glycoproteins and glycolipids is critical for mediating the binding of ligands, receptors, and macromolecules on the plasma membrane. However, we currently do not have the methods to quantify the spatial heterogeneities of macromolecular crowding on live cell surfaces. In this work, we combine experiment and simulation to report crowding heterogeneities on reconstituted membranes and live cell membranes with nanometer spatial resolution. By quantifying the effective binding affinity of IgG monoclonal antibodies to engineered antigen sensors, we discovered sharp gradients in crowding within a few nanometers of the crowded membrane surface. Our measurements on human cancer cells support the hypothesis that raft-like membrane domains exclude bulky membrane proteins and glycoproteins. Our facile and high-throughput method to quantify spatial crowding heterogeneities on live cell membranes may facilitate monoclonal antibody design and provide a mechanistic understanding of plasma membrane biophysical organization.
Unraveling Spatial-Spectral Dynamics of Speech Categorization Speed using Convolutional Neural Network
Authors: Moinuddin Kazi Ashraf , Havugimana Felix , Al-Fahad Rakib , Bidelman Gavin M , Yeasin Mohammed 
Published: November 2022

1AbstractThe process of categorizing sounds into distinct phonetic categories is known as categorical perception (CP). Response times (RTs) provide a measure of perceptual difficulty during labeling decisions (i.e., categorization). The RT is quasi-stochastic in nature due to individuality and variations in perceptual tasks. To identify the source of RT variation in CP, we have built models to decode the brain regions and frequency bands driving fast, medium and slow response decision speeds. In particular, we implemented parameter optimized convolutional neural network (CNN) to classify listeners behavioral RTs from their neural EEG data. We adopted visual interpretation of model response using Guided-GradCAM to identify spatial-spectral correlates of RT. Our framework includes (but is not limited to): (i) a data augmentation technique designed to reduce noise and control the overall variance of EEG dataset; (ii) bandpower topomaps to learn the spatial-spectral representation using CNN; (iii) large-scale Bayesian hyper-parameter optimization to find best performing CNN model; (iv) ANOVA and post-hoc analysis on Guided-GradCAM activation values to measure the effect of neural regions and frequency bands on behavioral responses. Using this framework, we observe thatα–β(10-20 Hz) activity over left frontal, right prefrontal/frontal, and right cerebellar regions are correlated with RT variation. Our results indicate that attention, template matching, temporal prediction of acoustics, motor control and decision uncertainty are the most probable factors in RT variation.
Effect of Blood Type on Mortality Among Patients with Brain Metastases
Authors: Zheng Yilong , Zhao Jiashen , Teo Kejia , Weng Nga Vincent Diong , Yeo Tseng Tsai , Rui Lim Mervyn Jun 
Published: November 2022

AbstractObjectiveABO blood type has been associated with mortality among patients with cancer, but this association has thus far not been investigated among patients with brain metastases. Hence, we aimed to investigate the association between ABO blood type and mortality among patients who underwent surgical resection of brain metastases.MethodsA single-center retrospective study of patients who underwent surgical resection of brain metastases between 2011 and 2019 was conducted. Cox proportional hazards models were constructed, adjusting for potential confounders, to evaluate whether blood type was independently associated with overall mortality.ResultsA total of 158 patients were included in the analysis. The mean (SD) age of the cohort was 59.3 (12.0) years, and 67.7% of patients were female. The median overall survival of patients with AB was 11.2 months, while the median overall survival of patients with blood types O, B, and A were 11.7, 13.5, and 14.4 months respectively. On multivariate analysis adjusting for potential confounders, there was a statistically significant association between blood type AB and a higher risk of overall mortality (HR=2.42, 95% CI=1.20, 4.86, p=0.013).ConclusionsBlood type AB was independently associated with a higher risk of overall mortality among patients who underwent surgical resection of brain metastases. We hypothesize that antibodies against blood type antigens A and/or B have anti-tumor activity in patients with brain metastases. Further studies are needed to validate our conclusions and hypothesis.
Stress-associated purinergic receptors code for fatal suicidality in the hippocampal-hypothalamic-prefrontal circuit
Authors: Zhang Lin , Verwer Ronald W.H. , van Heerikhuize Joop , Balesar Rawien , Correa-da-Silva Felipe , Slabe Zala , Lucassen Paul J. , Swaab Dick F. 
Published: November 2022

AbstractImbalanced purine metabolism is a key neurological basis for suicide and mood disorders (MD), wherein purinergic receptors in stress-sensitive cerebral regions are thought to be differentially activated. A hippocampal network that links the hypothalamus and prefrontal cortex implements an affective sensation of stress. We discovered that the hippocampus encoded fatal suicidal ideations in the dentate gyrus (DG) by a considerable amount of the granule cell nuclei with P2X purinoceptor 7 (P2RX7) expression, irrespective of the underlying MD. Compared to controls, patients with MD showed microglial dyshomeostasis throughout the hippocampal formation. Strikingly, P2Y purinoceptor 12 (P2RY12)-expressing microglia with segmented processes were remarkably present in the superficial layers of the medial entorhinal cortex (mEnt) in individuals with fatal suicidality. In the hypothalamic stress-sensitive nuclei, P2RY12+microglia were more expressed in the supraoptic nucleus in MD and even higher when fatal suicidality was present. In the prefrontal cortex, P2RX7 transcripts sharply dropped in suicidal individuals, possibly removing the prefrontal inhibition of the hippocampus and hypothalamus. Confounder analysis showed that the suicide-specific molecular features faded when the postmortem delay was prolonged. Our findings imply that fatal suicidality presents with unique neuropathological alterations. The DG and mEnt are two crucial areas for deciphering the suicidal consequences. By including brain samples from legal euthanasia donors, suicide-specific biosignatures can be maximally retained. Decoding the bioactive framework through key genes, brain regions and neurological processes involved in suicide neuropathology may provide novel therapeutic strategies for suicidal individuals who are beyond the reach of mental health care.
Mitochondrial defects leading to arrested spermatogenesis and ferroptosis in a mouse model of Leigh Syndrome
Authors: Radaelli Enrico , Assenmacher Charles-Antoine , Banerjee Esha , Manero Florence , Khiati Salim , Girona Anais , Lopez-Lluch Guillermo , Navas Placido , Spinazzi Marco 
Published: November 2022

ABSTRACTImpaired spermatogenesis and male infertility are common manifestations of mitochondrial diseases, but the underlying mechanisms are unclear. Here we show that mice deficient for PARL, the mitochondrial rhomboid protease, a recently reported model of Leigh syndrome, develop postpubertal testicular atrophy caused by arrested spermatogenesis and germ cell death independently of neurodegeneration. Genetic modifications of PINK1, PGAM5, and TTC19, three major substrates of PARL with important roles in mitochondrial homeostasis, do not reproduce or modify this phenotype. PARL deficiency in testis mitochondria leads to severe mitochondrial electron transfer chain defects, alterations in Coenzyme Q biosynthesis and redox status, and abrogates GPX4 expression specifically in spermatocytes leading to massive ferroptosis, an iron-dependent regulated cell death modality characterized by uncontrolled lipid peroxidation. Thus, mitochondrial defects can initiate ferroptosisin vivoin specific cell types by simultaneous effects on GPX4 and Coenzyme Q. These results highlight the importance of ferroptosis and cell-type specific downstream responses to mitochondrial deficits with respect to specific manifestations of mitochondrial diseases.
Heard or understood? Neural tracking of language features in a comprehensible story, an incomprehensible story and a word list
Authors: Gillis Marlies , Vanthornhout Jonas , Francart Tom 
Published: November 2022

AbstractSpeech comprehension is a complex neural process on which relies on activation and integration of multiple brain regions. In the current study, we evaluated whether speech comprehension can be investigated by neural tracking. Neural tracking is the phenomenon in which the brain responses time-lock to the rhythm of specific features in continuous speech. These features can be acoustic, i.e., acoustic tracking, or derived from the content of the speech using language properties, i.e., language tracking. We evaluated whether neural tracking of speech differs between a comprehensible story, an incomprehensible story, and a word list. We evaluated the neural responses to speech of 19 participants (6 men). No significant difference regarding acoustic tracking was found. However, significant language tracking was only found for the comprehensible story. The most prominent effect was visible to word surprisal, a language feature at the word level. The neural response to word surprisal showed a prominent negativity between 300 and 400 ms, similar to the N400 in evoked response paradigms. This N400 was significantly more negative when the story was comprehended, i.e., when words could be integrated in the context of previous words. These results show that language tracking can capture the effect of speech comprehension.Significance statementMost neural tracking studies focus on how the brain tracks acoustic speech features. However, whether acoustic tracking reflects speech comprehension is unclear. Therefore, in the pursuit of finding a neural marker for speech comprehension, language tracking might be a more suitable candidate. The results of this study showed that, indeed, language tracking can capture the effect of speech comprehension. This indicates that language tracking is a potential neural marker of speech comprehension. Such a neural marker would allow testing speech comprehension in populations that are currently difficult to test with behavioral tests, such as young children and persons with cognitive impairment.
High-fat diet feeding triggers a regenerative response in the adult zebrafish brain
Authors: Azbazdar Yagmur , Poyraz Yusuf Kaan , Ozalp Ozgun , Nazli Dilek , Ipekgil Dogac , Cucun Gokhan , Ozhan Gunes 
Published: November 2022

AbstractNon-alcoholic fatty liver disease (NAFLD) includes a range of liver conditions ranging from excess fat accumulation to liver failure. NAFLD is strongly associated with high-fat diet (HFD) consumption that constitutes a metabolic risk factor. While HFD has been elucidated concerning its several systemic effects, there is little information about its influence on the brain at the molecular level. Here, by using a high-fat diet (HFD)-feeding of adult zebrafish, we first reveal that excess fat uptake results in weight gain and fatty liver. Prolonged exposure to HFD induces a significant increase in the expression of pro-inflammation, apoptosis, and proliferation markers in the liver and brain tissues. Immunofluorescence analyses of the brain tissues disclose stimulation of apoptosis and widespread activation of glial cell response. Moreover, glial activation is accompanied by an initial decrease in the number of neurons and their subsequent replacement in the olfactory bulb and the telencephalon. Long-term consumption of HFD causes activation of Wnt/β-catenin signaling in the brain tissues. Finally, fish fed an HFD induces anxiety, and aggressiveness and increases locomotor activity. Thus, HFD feeding leads to a non-traumatic brain injury and stimulates a regenerative response. The activation mechanisms of a regeneration response in the brain can be exploited to fight obesity and recover from non-traumatic injuries.
MAP4K4 regulates biomechanical forces at adherens junctions and focal adhesions to promote collective cell migration
Authors: Alberici Delsin Lara Elis , Plutoni Cédric , Clouvel Anna , Keil Sarah , Marpeaux Léa , Elouassouli Lina , Khavari Adele , Ehrlicher Allen , Emery Gregory 
Published: November 2022

ABSTRACTCollective cell migration is important for normal development and tissue homeostasis, but can also promote cancer metastasis. To migrate collectively, cells need to coordinate their protrusion formation, rear retraction, adhesion sites dynamics, as well as forces generation and transmission. Nevertheless, the regulatory mechanisms coordinating these processes remain elusive. Using the A431 carcinoma cell line, we identify the kinase MAP4K4 as a central regulator of collective migration. We show that MAP4K4 inactivation blocks the migration of clusters while its overexpression decreases cluster cohesion. MAP4K4 regulates protrusion and retraction dynamics, remodels the actomyosin cytoskeleton, and controls the stability of both cell-cell and cell substrate adhesion. MAP4K4 promotes focal adhesion disassembly through the phosphorylation of Moesin, an actin and plasma membrane cross-linker, but disassembles adherens junctions through a Moesin-independent mechanism. By analyzing traction and intercellular forces, we found that the stabilization of adhesion sites in MAP4K4 loss of function leads to a tensional disequilibrium throughout the cell cluster, increasing the traction forces exerted onto the substrate and the tension loading at the cell-cell adhesions. Together, our results indicates that MAP4K4 activity is a key regulator of biomechanical forces at adhesion sites, promoting collective migration.
Strategies to streamline supplier relationship management during crises: lessons learned from COVID-19 and future pathways
Authors: Butt Atif Saleem , Alghababsheh Mohammad , Arshi Tahseen Anwer , Shah Syed Hamad Hassan 
Published: November 2022

PurposeThis paper explores the strategies adopted by purchasing firms to streamline relationships with suppliers amid the COVID-19 outbreak.Design/methodology/approachThis study employed a multiple case study method and conducted 42 semi-structured interviews with procurement managers from six firms in the United Arab Emirates.FindingsThis study reveals six helpful strategies that purchasing firms can undertake to streamline supplier relationship management (SRM) in the wake of COVID-19. Precisely, purchasing firms are revising supply chain costs, planning orders in advance, sharing critical information with suppliers, planning major contingencies, developing a robust relationship with suppliers (e.g. partnership) and finally, improving the supplier's visibility.Research limitations/implicationsThis study comes with certain limitations. First, the results are based on a limited number of 42 interviewees. Hence, the study’s results cannot be generalized to a broader population. Second, the data were collected based on the cause and effect relationship.Practical implicationsThe findings of this study can help purchasing firms learn and use new appropriate strategies to manage the relationship with their suppliers in the midst of the COVID-19 outbreak.Originality/valueThis study contributes to SRM literature by unveiling six distinct strategies (such as revising supply chain costs, planning orders in advance, sharing critical information, etc.) that purchasing firms have employed to develop a robust and healthy working relationship with the suppliers in the midst of the COVID-19 outbreak.