bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recent-onset and persistent tinnitus: Uncovering the differences in brain activities using resting-state functional magnetic resonance imaging technologies
Authors: Du Haoliang , Feng Xu , Qian Xiaoyun , Zhang Jian , Liu Bin , Li Ao , Huang Zhichun , Gao Xia 
Published: October 2022

ObjectiveThis study aimed to investigate the differences in intra-regional brain activity and inter-regional functional connectivity between patients with recent-onset tinnitus (ROT) and persistent tinnitus (PT) using resting-state functional magnetic resonance imaging (rs-fMRI), including the amplitude of low-frequency fluctuations (ALFF), regional homogeneity (ReHo), and voxel-wise functional connectivity (FC).MethodWe acquired rs-fMRI scans from 82 patients (25 without recent-onset tinnitus, 28 with persistent tinnitus, and 29 healthy controls). Age, sex, and years of education were matched across the three groups. We performed ALFF, ReHo, and voxel-wise FC analyses for all patients.ResultsCompared with the control group, participants with ROT and PT manifested significantly reduced ALFF and ReHo activity within the left and right dorsolateral superior frontal gyrus (SFG) and gyrus rectus (GR). Additional voxel-wise FC revealed decreased connectivity between the dorsolateral SFG (left and right) and the right superior parietal gyrus (SPG), right middle frontal gyrus (MFG), and left medial superior frontal gyrus (mSFG) within these two groups. Significant differences were observed between the ROT and PT groups, with the ROT group demonstrating reduced FC.ConclusionOur data suggest that patients with PT have more difficulty monitoring external stimuli and reorienting attention than patients with ROT. In addition, patients who perceive higher levels of disruption from tinnitus are more likely to develop persistent and debilitating tinnitus once the tinnitus lasts longer than six months. Therefore, we strongly recommend that clinicians implement effective tinnitus management strategies in patients with ROT as soon as possible.
Development of genomic instability-associated long non-coding RNA signature: A prognostic risk model of clear cell renal cell carcinoma
Authors: Jiang Dongfang , Wu Tiange , Shi Naipeng , Shan Yong , Wang Jinfeng , Jiang Hua , Wu Yuqing , Wang Mengxue , Li Jian , Liu Hui , Chen Ming 
Published: October 2022

PurposeRenal clear cell carcinoma (ccRCC) is the most lethal of all pathological subtypes of renal cell carcinoma (RCC). Genomic instability was recently reported to be related to the occurrence and development of kidney cancer. The biological roles of long non-coding RNAs (lncRNAs) in tumorigenesis have been increasingly valued, and various lncRNAs were found to be oncogenes or cancer suppressors. Herein, we identified a novel genomic instability-associated lncRNA (GILncs) model for ccRCC patients to predict the overall survival (OS).MethodsThe Cancer Genome Atlas (TCGA) database was utilized to obtain full transcriptome data, somatic mutation profiles, and clinical characteristics. The differentially expressed lncRNAs between the genome-unstable-like group (GU) and the genome-stable-like group (GS) were defined as GILncs, with |logFC| &gt; 1 and an adjusted p-value&lt; 0.05 for a false discovery rate. All samples were allocated into GU-like or GS-like types based on the expression of GILncs observed using hierarchical cluster analyses. A genomic instability-associated lncRNA signature (GILncSig) was constructed using parameters of the included lncRNAs. Quantitative real-time PCR analysis was used to detect the in vitro expression of the included lncRNAs. Validation of the risk model was performed by the log-rank test, time-dependent receiver operating characteristic (ROC) curves analysis, and multivariate Cox regression analysis.ResultsForty-six lncRNAs were identified as GILncs. LINC00460, AL139351.1, and AC156455.1 were employed for GILncSig calculation based on the results of Cox analysis. GILncSig was confirmed as an independent predictor for OS of ccRCC patients. Additionally, it presented a higher efficiency and accuracy than other RCC prognostic models reported before.ConclusionGILncSig score was qualified as a critical indicator, independent of other clinical factors, for prognostic prediction of ccRCC patients.
The effect of a traditional Chinese quadri-combination therapy and its component quercetin on recurrent spontaneous abortion: A clinical trial, network pharmacology and experiments-based study
Authors: Zhou Jing , Li Lisha , Pan Xinyao , Wang Jing , Qi Qing , Sun Hongmei , Li Chuyu , Wang Ling 
Published: October 2022

Objective: To explore the effect and mechanisms of a traditional Chinese quadri-combination therapy [Bushen, Yiqi, Lixue and Yangtai (BYLY)] in treating recurrent spontaneous abortion (RSA).Methods: A clinical trial was conducted to study the effect of BYLY on RSA. Pharmacological network analysis and UPLC-Q/TOF-mass spectrometry (MS) were applied to investigate the key active component in BYLY and potential targets. Cellular experiments based on former results were performed to examine the mechanism of BYLY in the treatment of RSA.Results: Four hundred and eighty participants enrolled in the clinical trial. The results showed that, compared with the use of BYLY or duphaston alone, a combination of duphaston and BYLY could decrease the early abortion rate in RSA (p &lt; 0.001). Network pharmacological analysis indicated that BYLY contained 132 active components and 146 core targets, and the quercetin maybe the key effective component. In vitro experiments found that pretreatment of quercetin at the correct concentration (2 μM) prevented hypoxia-induced viability and proliferation reduction, and apoptosis and mitochondrial dysfunction. Furthermore, quercetin could modulate mitochondrial fission/fusion balance in trophoblasts, and specifically decrease the expression of Drp1 by regulating miR-34a-5p.Conclusion: BYLY could improve pregnancy outcomes of RSA, based on multi-components and multi-targets. The protective effect of quercetin on trophoblasts, through decreasing Drp1 expression via regulating miR-34a-5p, might be one possible effective mechanism.
Selection and validation of reference genes for RT-qPCR-based analyses of Anastatus japonicus Ashmead (Hymenoptera: Helicopteridae)
Authors: Liu Zixin , Xiao Junjiang , Xia Yue , Wu Qifeng , Zhao Can , Li Dunsong 
Published: October 2022

RT-qPCR remains a vital approach for molecular biology studies aimed at quantifying gene expression in a range of physiological or pathological settings. However, the use of appropriate reference genes is essential to attain meaningful RT-qPCR results. Anastatus japonicus Ashmead (Hymenoptera: Helicopteridae) is an important egg parasitoid wasp and natural enemy of fruit bugs and forest caterpillars. While recent transcriptomic studies have analyzed gene expression profiles in A. japonicus specimens, offering a robust foundation for functional research focused on this parasitoid, no validated A. japonicus reference genes have yet been established, hampering further research efforts. Accordingly, this study sought to address this issue by screening for the most stable internal reference genes in A. japonicus samples to permit reliable RT-qPCR analyses. The utility of eight candidate reference genes (ACTIN, TATA, GAPDH, TUB, RPL13, RPS6, EF1α, RPS3a) was assessed under four different conditions by comparing developmental stages (larvae, pupae, adults), tissues (abdomen, chest, head), sex (male or female adults), or diapause states (diapause induction for 25, 35, 45, or 55 days, or diapause termination). RefFinder was used to calculate gene stability based on the integration of four algorithms (BestKeeper, Normfinder, geNorm, and ΔCt method) to determine the optimal RT-qPCR reference gene. Based on this approach, RPS6 and RPL13 were found to be the most reliable reference genes when assessing different stages of development, while ACTIN and EF1α were optimal when comparing adults of different sexes, RPL13 and EF1α were optimal when analyzing different tissues, and TATA and ACTIN were optimal for different diapause states. These results provide a valuable foundation for future RT-qPCR analyses of A. japonicus gene expression and function under a range of experimental conditions.
Exercise therapy in the treatment of anorexia nervosa: Its effects depending on the type of physical exercise—A systematic review
Authors: Toutain Marc , Gauthier Antoine , Leconte Pascale 
Published: October 2022

Background and purposeClinical research focusing on the effectiveness of exercise therapy (ET) in patients with anorexia nervosa (AN) shows increasing interest in the last decade. The aim of this systematic review was to provide an overview of quantitative studies that have examined the impact of ET in AN patients and to examine its specific effects on physical and mental health according to the type of physical exercise (PE) practiced.MethodsThe review was carried out based on the PRISMA 2020. Electronic databases PubMed, Web of Science, Embase, and Wiley were searched from inception to December 2021. Quantitative studies assessing the effects of ET interventions on AN patients were included and study quality was assessed using the PEDro scale.ResultsA total of 27 studies were selected, including 13 randomized controlled trials. Regarding outcomes measured, results showed that aerobic and resistance exercise improved muscle strength, that mind-body PE decreased main symptoms of AN and mental health, and that combined PE reduced dysfunctional exercise and improved weight gain.ConclusionThe findings suggest that ET intervention can induce benefits and has no deleterious effects on patients. In addition, specific effects on anorexia symptoms and physical and mental health have been observed according to the type of PE. However, this review reported several methodological weaknesses, including a lack of control group or randomization and statistical misconduct. Finally, ET intervention parameters were heterogeneous, and ET intervention generally lacked details, making reproducibility and comparability difficult. All these limitations underscore the need for a more rigorous methodology for further research.
Association between sedentary behavior, physical activity, and cardiovascular disease-related outcomes in adults—A meta-analysis and systematic review
Authors: Liang Zhi-de , Zhang Meng , Wang Chuan-zhi , Yuan Yang , Liang Jing-hong 
Published: October 2022

BackgroundSedentary behavior (SB) and physical activity (PA) are modifiable risk factors for cardiovascular disease (CVD); however, previous research on the effects of PA and SB on CVD has been relatively homogeneous. Our study investigated the association between PA, SB, and CVD-related outcomes.MethodsA comprehensive search strategy was conducted in the MEDLINE, Embase, Cochrane Library, and Web of Science databases from their inception to September 2022. We identified eligible studies according to PICOS: the populations comprised healthy adults, the interventions or exposures were PA or SB, the outcomes were CVD-related outcomes, and the study designs were randomized controlled trials (RCTs) and longitudinal studies (LS). Outcomes were pooled using fixed or random effects models, and the quality of individual studies was assessed by the Cochrane Risk of Bias Instrument and the Newcastle Ottawa Scale.ResultsA total of 148 RCTs and 36 LS were included, comprising a total of 75,075 participants. The study quality was rated as low to moderate. We found an increased hazard ratio (HR) for CVD in the population with SB (HR = 1.34; 95% confidence interval [CI]: 1.26 to 1.43; I2 = 52.3%; Pheterogeneity &lt; 0.001, random model) and a decreased HR for CVD in those who performed long-term PA (HR = 0.71; 95% CI: 0.66 to 0.77; I2 = 78.0%, Pheterogeneity &lt; 0.001, random model). Long-term PA improved the lipid profiles in healthy adults; participants in this group exhibited increased high-density lipoprotein (weighted mean difference [WMD] = 2.38; 95% CI: 1.00 to 3.76; I2 = 84.7%; Pheterogeneity &lt; 0.001, random model), decreased triglycerides (WMD = −7.27; 95% CI: −9.68 to −4.87; I2 = 0%, Pheterogeneity = 0.670, fixed model), and lower total-cholesterol (WMD = −6.84; 95% CI: −9.15 to −4.52; I2 = 38.4%, Pheterogeneity &lt; 0.001, random model).ConclusionsLong-term SB increases the risk of CVD in healthy adults, whereas PA reduces the risk of CVD and improves indicators associated with CVD. However, the ability of PA to improve blood lipids appeared to be limited. The detailed association of SB and PA on CVD needs to be further investigated in the future.
Development and internal validation of a risk model for hyperuricemia in diabetic kidney disease patients
Authors: Huang Guoqing , Li Mingcai , Mao Yushan , Li Yan 
Published: October 2022

PurposeThis research aimed to identify independent risk factors for hyperuricemia (HUA) in diabetic kidney disease (DKD) patients and develop an HUA risk model based on a retrospective study in Ningbo, China.Patients and methodsSix hundred and ten DKD patients attending the two hospitals between January 2019 and December 2020 were enrolled in this research and randomized to the training and validation cohorts based on the corresponding ratio (7:3). Independent risk factors associated with HUA were identified by multivariable logistic regression analysis. The characteristic variables of the HUA risk prediction model were screened out by the least absolute shrinkage and selection operator (LASSO) combined with 10-fold cross-validation, and the model was presented by nomogram. The C-index and receiver operating characteristic (ROC) curve, calibration curve and Hosmer–Lemeshow test, and decision curve analysis (DCA) were performed to evaluate the discriminatory power, degree of fitting, and clinical applicability of the risk model.ResultsBody mass index (BMI), HbA1c, estimated glomerular filtration rate (eGFR), and hyperlipidemia were identified as independent risk factors for HUA in the DKD population. The characteristic variables (gender, family history of T2DM, drinking history, BMI, and hyperlipidemia) were screened out by LASSO combined with 10-fold cross-validation and included as predictors in the HUA risk prediction model. In the training cohort, the HUA risk model showed good discriminatory power with a C-index of 0.761 (95% CI: 0.712–0.810) and excellent degree of fit (Hosmer–Lemeshow test, P &gt; 0.05), and the results of the DCA showed that the prediction model could be beneficial for patients when the threshold probability was 9–79%. Meanwhile, the risk model was also well validated in the validation cohort, where the C-index was 0.843 (95% CI: 0.780–0.906), the degree of fit was good, and the DCA risk threshold probability was 7–100%.ConclusionThe development of risk models contributes to the early identification and prevention of HUA in the DKD population, which is vital for preventing and reducing adverse prognostic events in DKD.
Sinfonia: Cross-tier orchestration for edge-native applications
Authors: Satyanarayanan Mahadev , Harkes Jan , Blakley Jim , Meunier Marc , Mohandoss Govindarajan , Friedt Kiel , Thulasi Arun , Saxena Pranav , Barritt Brian 
Published: October 2022

The convergence of 5G wireless networks and edge computing enables new edge-native applications that are simultaneously bandwidth-hungry, latency-sensitive, and compute-intensive. Examples include deeply immersive augmented reality, wearable cognitive assistance, privacy-preserving video analytics, edge-triggered serendipity, and autonomous swarms of featherweight drones. Such edge-native applications require network-aware and load-aware orchestration of resources across the cloud (Tier-1), cloudlets (Tier-2), and device (Tier-3). This paper describes the architecture of Sinfonia, an open-source system for such cross-tier orchestration. Key attributes of Sinfonia include:support for multiple vendor-specific Tier-1 roots of orchestration, providing end-to-end runtime control that spans technical and non-technical criteria;use of third-party Kubernetes clusters as cloudlets, with unified treatment of telco-managed, hyperconverged, and just-in-time variants of cloudlets;masking of orchestration complexity from applications, thus lowering the barrier to creation of new edge-native applications.We describe an initial release of Sinfonia (https://github.com/cmusatyalab/sinfonia), and share our thoughts on evolving it in the future.
Do prognosis and clinicopathological features differ in young early-stage breast cancer?
Authors: Arikan Akif Enes , Kara Halil , Dülgeroğlu Onur , Erdoğan Esin Nur , Capkinoglu Emir , Uras Cihan 
Published: October 2022

BackgroundBreast cancer is the most frequently detected cancer and the leading cause of cancer-related death in women. Although it is mostly seen in older patients, breast cancer affects women aged 24 to &gt;70 years, with poorer prognosis in young patients. Young age remains a controversial topic in the literature. This study aimed to identify subtype differences and the effect of age on early-stage breast cancer outcomes.MethodsA total of 300 consecutive patients underwent surgery between 2011 and 2015 for early-stage breast cancer. Of these, 248 were eligible for this study and were divided into three groups: group Y (aged ≤35 years), group M (aged &gt;35 and ≤45 years), and group E (aged &gt;45 years). The clinical and pathological features and data related to recurrence, metastasis, and death were recorded.ResultsNo statistical differences were found between groups regarding histopathological features except for higher histological grade and Ki-67 levels in group M. Additionally, group Y recorded no progression (recurrence or metastasis) or death. Disease-free survival was 117.8 months (95% CI 111.8–123.8) for group M, which was significantly shorter than that for group E (p &lt; 0.001). Additionally, the hazard ratio (HR) for progression from group M to group E was 10.21 with significant difference (p = 0.003, 95% CI 2.26–46.08). However, the HR of group Y to group E was 0.04, without significance (p = 0.788, 95% CI 0.18–345 × 106). The overall 5-year survival was 100% in group Y, 98.8% in group M, and 99.3% in group E, without significance.ConclusionA very young age cannot be considered an independent risk factor for poor prognosis. Rather than age, histological grade and Ki-67 index are more important factors in early-stage breast cancer.
Percepções sobre a utilização das teorias da aprendizagem em um curso de formação de professores para séries iniciais
Authors: Nich Rozemara , Chaves Taniamara Vizzotto , Bolzan Divane Bacin , Brittes Letícia Ramalho 
Published: October 2022

Este trabalho tem como propósito apresentar uma reflexão teórica acerca da observação de uma reunião de orientação de estágio em uma escola estadual no estado do Rio Grande do Sul, tomando por base as contribuições acadêmicas e as principais orientações recebidas na disciplina de Teorias e Práticas de Ensino e Aprendizagem do Curso de Mestrado em Educação Profissional e Tecnológica - Campus Jaguari. O propósito central deste trabalho foi perceber se, e como as teorias da aprendizagem em educação surgem em uma atividade prática de orientação de estágio, tais questões são apresentadas e discutidas à luz de referenciais teóricos que pretendem não só entender a importância das diversas filosofias de educação como também pensar as práticas pedagógicas hoje desenvolvidas nas salas de aula. Ainda que iniciais, estas reflexões apontam para a necessidade de muito comprometimento para com a atividade de formação de docentes, atentando para a aprendizagem inicial e sua complexa condição.
Toxicidade ocular induzida pelo tamoxifeno: uma revisão de literatura
Authors: Silva Thalita Paula de Oliveira , Jayme Brenda Cavalieri , Barreto Lara Marques , De Almeida Samir de Castilhos , Tomaz Paloma Nicolau , Vieira Raquel Morales , Lemos Luanna Arruda , Fraga Murillo Sidney Garcia , Dos Santos Juliene César , Ribeiro Gabriella Reis de Barros , Couto Liana Aguiar , Coury Heloá de Paula Telles , Damasceno Lucas Barbosa , Cunha Maria Luiza Hajjar , Pedrosa Ricardo Lima 
Published: October 2022

O tamoxifeno é um agente antiestrogênico que atua diretamente nas mamas, se ligando com receptores dos estrógenos e impedindo assim a sua ligação com o hormônio em questão, e é usado amplamente no tratamento do câncer de mama estrogênio positivo. Apesar de sua eficácia e baixos índices de efeitos colaterais, essa droga possui a capacidade de induzir toxicidade ocular em pacientes que fazem o seu uso diário, mesmo que em baixas doses. O objetivo desse estudo foi analisar quais são os principais efeitos colaterais oculares induzidos pelo tamoxifeno, bem como sua incidência e manejo. Para a confecção desse estudo, foram realizadas buscas em bases de dados e selecionados inicialmente 29 artigos, dentre os quais 17 se enquadraram nos critérios pré-estabelecidos. Como justificativa para a realização da pesquisa, destaca-se a escassez de estudos acerca desse tema na literatura mundial – sendo este estudo um dos poucos realizados por pesquisadores brasileiros – e a relevância clínica do problema em questão. O uso do tamoxifeno está relacionado diretamente com a toxicidade ocular, sendo a principal manifestação a retinopatia, seguida por alterações na córnea e neurite óptica. Apesar de rara, a potencial toxicidade ocular do tamoxifeno pode levar a eventos graves como diminuição irreversível da acuidade visual e até perda da visão.
Desenvolvimento de dispositivo elétrico capaz de atrair e capturar mosquitos transmissores de arboviroses por meio de luzes ultravioleta no município de Foz do Iguaçu
Authors: Oliveira João Victor de Castro , Santos Beatriz da Graça dos , Castro Miriã Rodrigues , Goulart Gabriel Feliphe Bertuchi , Bandeira Ester , Pereira Silviane Galvan 
Published: October 2022

Introdução: As arboviroses, que são doenças infecciosas propagadas por mosquitos, estão se tornando um problema de saúde pública que necessita cada vez mais atenção de órgãos públicos e similarmente da própria população, tendo em vista a ascendência dessas enfermidades. Objetivo: Destarte, o artigo objetivou-se na relação das arboviroses no município de Foz de Iguaçu, e no desenvolvimento de um dispositivo para a captura de mosquitos, para auxiliar na redução das arboviroses no campo. Metodologia: Foi desenvolvida a armadilha que atuou na atração e captura dos vetores, por meio do uso de diodos emissores de luz ultravioleta, a qual possui eficácia comprovada na atração foi desenvolvida, estudada e monitorada com intuito de verificar a existência e a quantidade de espécies transmissoras de arboviroses. Resultados: No decorrer da identificação dos mosquitos capturados, foram contabilizados 161 hematófagos. Conclusões: Por fim, além do uso do dispositivo elétrico, será necessário incentivar a educação e a prevenção dessas arboviroses, voltadas à população do município de Foz, de modo que a prevalência destas doenças possa ser devidamente reduzida.