bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential maternal health service disruptions in Ethiopia during COVID 19 pandemic: a systematic review
Authors: Zewdie Amare , Mose Ayenew , Yimer Ali , Melis Tamirat , Muhamed Ahmed Nuru , Jemal Abdurezak Kemal 
Published: December 2022

Abstract
Introduction
COVID 19 pandemic has challenged the resilience of the most effective health systems in the world. The Ethiopian Ministry of health tried to ensure the continuation of essential maternal health services during the pandemic. Despite several individual studies conducted on the impact of COVID 19 on maternal health services, no evidence can summarize the extent of impact as a nation and which essential maternal health service is most affected.

Method
A systematic review was conducted to summarize the extent of disruption of essential maternal health services and identify the most affected service in the era of the COVID pandemic in Ethiopia. Preferred Reporting Items for Systematic Review and Meta-analysis guidelines were followed. Comprehensive literature was searched using international databases PubMed, Google scholar, and African Online Journal to retrieve related articles. Descriptive analysis was made to answer the review objective.

Result
Overall, 498 articles were retrieved using our search strategy and finally 8 articles were included in the review. We found, ANC (26.35%), skilled birth attendance (23.46%), PNC (30%), family planning (14%), and abortion care (23.7%) maximum disruption of service utilization due to the pandemic. PNC service was the most significantly affected service unit followed by the ANC unit.

Conclusion
Essential maternal health services have been significantly disrupted due to COVID 19 pandemic in Ethiopia. It is expected from all stakeholders to prioritize safe and accessible maternity care during the pandemic and the aftermath and take lesson to reduce maternal and infant morbidity and mortality.
Pharmacokinetics and pharmacodynamics of bioactive compounds in Penyanqing preparation in THP-1 inflammatory cells induced by Lipopolysaccharide
Authors: Gong Linna , Miao Zhishuo , Zhang Li , Shi Birui , Xiao Zuoqi , Qiu Panzi , Liu Menghua , Zou Wei 
Published: December 2022

Abstract
Background
Penyanqing (PYQ), a traditional Chinese medicine (TCM), has a good clinical efficacy for the treatment of pelvic inflammatory disease (PID). Previously, researches on its anti-inflammatory effect and mechanism in vitro, in silico, and in vivo have been reported by our team. However, the interrelationship between the anti-inflammatory activity and the active compounds in PYQ are not clear. Here, the pharmacokinetics-pharmacodynamics (PK-PD) study was carried out for more proper clinical use.

Methods
The plasma concentrations of salvianolic acid B (SAB), protocatechualdehyde (PRO), paeoniflorin (PE), astilbin (AST), ferulic acid (FE), and chlorogenic acid (CH) in SD rats after PYQ administration were determined by a selective and rapid HPLC–MS/MS method. In addition, the PK-PD on cell model was used to explore the relationship between the plasma concentration and inflammatory biomarkers (TNF-α, IL-1β).

Results
The results of this study showed that the six components could reach the peak blood concentration within 0.29 h, indicating the rapid absorption of it. The eliminations of AST, CH, FE, PE, and PRO were relatively fast due to their mean residence times (MRTs) within 3 h, while the elimination of SAB was slower (MRT 5.67 ± 0.66 h). Combined with a THP-1 cell model, there was a significant correlation between inflammatory factors and component plasma concentrations with correlation coefficients in the range of -0.9—-0.746. Correspondingly, the drug-containing plasma obtained at 0.25 h point exhibited the best inhibition effect on production of IL-1β and TNF-α in LPS-induced THP-1 cells.

Conclusion
The six main components in PYQ could be quickly absorbed, and there was a potential good correlation between their pharmacokinetics and the pharmacodynamics of PYQ.
Ideational factors associated with consistent use of insecticide-treated nets: a multi-country, multilevel analysis
Authors: Babalola Stella , Kumoji Kuor , Awantang Grace N. , Oyenubi Olamide A. , Toso Michael , Tsang Samantha , Bleu Therese , Achu Dorothy , Hedge Judith , Schnabel David C. , Cash Shelby , Van Lith Lynn M. , McCartney-Melstad Anna C. , Nkomou Yannick , Dosso Abdul , Lahai Wani , Hunter Gabrielle C. 
Published: December 2022

AbstractBackgroundMalaria remains a major cause of morbidity and mortality in sub-Saharan Africa. Using insecticide-treated nets (ITNs) every night, year-round is critical to maximize protection against malaria. This study describes sociodemographic, psychosocial, and household factors associated with consistent ITN use in Cameroon, Côte d’Ivoire and Sierra Leone.MethodsCross-sectional household surveys employed similar sampling procedures, data collection tools, and methods in three countries. The survey sample was nationally representative in Côte d’Ivoire, representative of the North and Far North regions in Cameroon, and representative of Bo and Port Loko districts in Sierra Leone. Analysis used multilevel logistic regression and sociodemographic, ideational, and household independent variables among households with at least one ITN to identify correlates of consistent ITN use, defined as sleeping under an ITN every night the preceding week.FindingsConsistent ITN use in Côte d’Ivoire was 65.4%, 72.6% in Cameroon, and 77.1% in Sierra Leone. While several sociodemographic and ideational variables were correlated with consistent ITN use, these varied across countries. Multilevel logistic regression results showed perceived self-efficacy to use ITNs and positive attitudes towards ITN use were variables associated with consistent use in all three countries. The perception of ITN use as a community norm was positively linked with consistent use in Cameroon and Côte d’Ivoire but was not significant in Sierra Leone. Perceived vulnerability to malaria was positively linked with consistent use in Cameroon and Sierra Leone but negatively correlated with the outcome in Côte d’Ivoire. Household net sufficiency was strongly and positively associated with consistent use in all three countries. Finally, the findings revealed strong clustering at the household and enumeration area (EA) levels, suggesting similarities in net use among respondents of the same EA and in the same household.ConclusionsThere are similarities and differences in the variables associated with consistent ITN use across the three countries and several ideational variables are significant. The findings suggest that a social and behaviour change strategy based on the ideation model is relevant for increasing consistent ITN use and can inform specific strategies for each context. Finally, ensuring household net sufficiency is essential.
TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein
Authors: Yang Xu , Zhang Yan , Xue Zhiwei , Hu Yaotian , Zhou Wenjing , Xue Zhiyi , Liu Xuemeng , Liu Guowei , Li Wenjie , Liu Xiaofei , Li Xingang , Han Mingzhi , Wang Jian 
Published: December 2022

Abstract
Background
The tripartite motif (TRIM) family of proteins plays a key role in the developmental growth and therapeutic resistance of many tumors. However, the regulatory mechanisms and biological functions of TRIM proteins in human glioblastoma (GBM) are not yet fully understood. In this study, we focused on TRIM56, which emerged as the most differentially expressed TRIM family member with increased expression in GBM.

Methods
Western blot, real-time quantitative PCR (qRT-PCR), immunofluorescence (IF) and immunohistochemistry (IHC) were used to study the expression levels of TRIM56 and cIAP1 in GBM cell lines. Co-immunoprecipitation (co-IP) was used to explore the specific binding between target proteins and TRIM56. A xenograft animal model was used to verify the tumor promoting effect of TRIM56 on glioma in vivo.

Results
We observed elevated expression of TRIM56 in malignant gliomas and revealed that TRIM56 promoted glioma progression in vitro and in a GBM xenograft model in nude mice. Analysis of the Human Ubiquitin Array and co-IPs showed that cIAP1 is a protein downstream of TRIM56. TRIM56 deubiquitinated cIAP1, mainly through the zinc finger domain (amino acids 21–205) of TRIM56, thereby reducing the degradation of cIAP1 and thus increasing its expression. TRIM56 also showed prognostic significance in overall survival of glioma patients.

Conclusions
TRIM56-regulated post-translational modifications may contribute to glioma development through stabilization of cIAP1. Furthermore, TRIM56 may serve as a novel prognostic indicator and therapeutic molecular target for GBM.
Extended-release of doxorubicin through green surface modification of gold nanoparticles: in vitro and in ovo assessment
Authors: Asariha Maryam , Kiaie Seyed Hossein , Izadi Sepideh , H. Pirhayati Faezeh , Fouladi Mehdi , Gholamhosseinpour Maryam 
Published: December 2022

AbstractIn the present study, a green surface modification of gold nanoparticles (GNPs) using chondroitin sulfate (CHS) and chitosan (CS) to deliver an extended-release of doxorubicin (DOX) was proposed. Following synthesis of each step of unconjugated counterpart, including CHS-GNPs, DOX–CHS–GNP, and conjugated construct DOX–CHS–GNP-CS, physicochemical properties of the nanoparticles (NPs) were characterized by FT-IR, DLS, and TEM analyses, and the release of DOX was determined by using UV–Vis spectrometry. Then, NPs were effectively taken up by MDA-MB-468, βTC-3, and human fibroblast (HFb) cell lines with high release percent and without significant cytotoxicity. The DOX–CHS–GNPs and DOX–CHS–GNP-CS NPs showed a mean size of 175.8 ± 1.94 and 208.9 ± 2.08 nm; furthermore, a zeta potential of − 34 ± 5.6 and − 25.7 ± 5.9 mV, respectively. The highest release of DOX was 73.37% after 45 h, while in the absence of CS, the release of DOX was 76.05% for 24 h. Compared to CHS-GNPs, the presence of CS decreased the rate of sustained release of DOX and improved the drug release efficiency. The results demonstrated an excellent release and negligible cytotoxicity at high concentrations of CHS-GNP-CS. Consequently, in ovo assessment corroborated the efficacy of the green fabricated NPs proposed effective targeted delivery of DOX for anti-tumor therapy in vitro.
Graphical Abstract
Prospects of thermotolerant Kluyveromyces marxianus for high solids ethanol fermentation of lignocellulosic biomass
Authors: Sengupta Priya , Mohan Ramya , Wheeldon Ian , Kisailus David , Wyman Charles E. , Cai Charles M. 
Published: December 2022

AbstractSimultaneous saccharification and fermentation (SSF) is effective for minimizing sugar inhibition during high solids fermentation of biomass solids to ethanol. However, fungal enzymes used during SSF are optimal between 50 and 60 °C, whereas most fermentative yeast, such as Saccharomyces cerevisiae, do not tolerate temperatures above 37 °C. Kluyveromyces marxianus variant CBS 6556 is a thermotolerant eukaryote that thrives at 43 °C, thus potentially serving as a promising new host for SSF operation in biorefineries. Here, we attempt to leverage the thermotolerance of the strain to demonstrate the application of CBS 6556 in a high solids (up to 20 wt% insoluble solid loading) SSF configuration to understand its capabilities and limitations as compared to a proven SSF strain, S. cerevisiae D5A. For this study, we first pretreated hardwood poplar chips using Co-Solvent Enhanced Lignocellulosic Fractionation (CELF) to remove lignin and hemicellulose and to produce cellulose-enriched pretreated solids for SSF. Our results demonstrate that although CBS 6556 could not directly outperform D5A, it demonstrated similar tolerance to high gravity sugar solutions, superior growth rates at higher temperatures and higher early stage ethanol productivity. We discovered that CBS 6556’s membrane was particularly sensitive to higher ethanol concentrations causing it to suffer earlier fermentation arrest than D5A. Cross-examination of metabolite data between CBS 6556 and D5A and cell surface imaging suggests that the combined stresses of high ethanol concentrations and temperature to CBS 6556’s cell membrane was a primary factor limiting its ethanol productivity. Hence, we believe K. marxianus to be an excellent host for future genetic engineering efforts to improve membrane robustness especially at high temperatures in order to achieve higher ethanol productivity and titers, serving as a viable alternative to D5A.
Supporting dialysis policy for end stage renal disease (ESRD) in Indonesia: an updated cost-effectiveness model
Authors: Putri Septiara , Nugraha Ryan R. , Pujiyanti Eka , Thabrany Hasbullah , Hasnur Hanifah , Istanti Novita D. , Evasari Diah , Afiatin  
Published: December 2022

Abstract
Objective
Continuous ambulatory peritoneal dialysis (CAPD) and hemodialysis (HD) are main modalities for end stage renal disease (ESRD) patients, and those have been covered by National Health Insurance (NHI) scheme since 2014 in Indonesia. This study aims to update the cost-effectiveness model of CAPD versus HD in Indonesia setting.

Results
Compared to HD, CAPD provides good value for money among ESRD patients in Indonesia. Using societal perspective, the total costs were IDR 1,348,612,118 (USD 95,504) and IDR 1,368,447,750 (USD 96,908), for CAPD and HD, respectively. The QALY was slightly different between two modalities, 4.79 for CAPD versus 4.22 for HD. The incremental cost-effectiveness ratio (ICER) yields savings of IDR 34,723,527/QALY (USD 2460).
Improving the diagnosis of acute ischemic stroke on non-contrast CT using deep learning: a multicenter study
Authors: Chen Weidao , Wu Jiangfen , Wei Ren , Wu Shuang , Xia Chen , Wang Dawei , Liu Daliang , Zheng Longmei , Zou Tianyu , Li Ruijiang , Qi Xianrong , Zhang Xiaotong 
Published: December 2022

Abstract
Objective
This study aimed to develop a deep learning (DL) model to improve the diagnostic performance of EIC and ASPECTS in acute ischemic stroke (AIS).

Methods
Acute ischemic stroke patients were retrospectively enrolled from 5 hospitals. We proposed a deep learning model to simultaneously segment the infarct and estimate ASPECTS automatically using baseline CT. The model performance of segmentation and ASPECTS scoring was evaluated using dice similarity coefficient (DSC) and ROC, respectively. Four raters participated in the multi-reader and multicenter (MRMC) experiment to fulfill the region-based ASPECTS reading under the assistance of the model or not. At last, sensitivity, specificity, interpretation time and interrater agreement were used to evaluate the raters’ reading performance.

Results
In total, 1391 patients were enrolled for model development and 85 patients for external validation with onset to CT scanning time of 176.4 ± 93.6 min and NIHSS of 5 (IQR 2–10). The model achieved a DSC of 0.600 and 0.762 and an AUC of 0.876 (CI 0.846–0.907) and 0.729 (CI 0.679–0.779), in the internal and external validation set, respectively. The assistance of the DL model improved the raters’ average sensitivities and specificities from 0.254 (CI 0.22–0.26) and 0.896 (CI 0.884–0.907), to 0.333 (CI 0.301–0.345) and 0.915 (CI 0.904–0.926), respectively. The average interpretation time of the raters was reduced from 219.0 to 175.7 s (p = 0.035). Meanwhile, the interrater agreement increased from 0.741 to 0.980.

Conclusions
With the assistance of our proposed DL model, radiologists got better performance in the detection of AIS lesions on NCCT.
What evidence exists of crop plants response to exposure to static magnetic and electromagnetic fields? A systematic map protocol
Authors: Pawełek Agnieszka , Owusu Samuel Acheaw , Cecchetti Daniele , Zielińska Adrianna , Wyszkowska Joanna 
Published: December 2022

AbstractBackgroundIncreasing demand for food and concerns over the environmental impact of agriculture has prompted the search for alternatives to many conventional farming practices. Reports on exposing seeds and plants at various developmental stages to static magnetic field (SMF) and non-ionizing electromagnetic fields (EMF) as a form of priming indicate some positive effects on seed germinability, growth rate, resistance to stress conditions, and improved yield. However, there exist some inconsistent reported treatment protocols and contradictory study outcomes that make it difficult to draw objective conclusions on the potential use of SMF and EMF as sustainable alternatives to improving crop growth and yield. It is equally essential to understand any adverse effects of exposing plants to SMF and EMF considering the abundance of their sources in the environment. In order to provide a more coherent overview of how plants respond to exposure to SMF and EMF not only in their observed effects of agronomic importance but also in the mechanisms of action of SMF and EMF in plant cells, we prepare a systematic map.MethodsLiterature will be identified by searching six bibliographic databases and three web-based search engines using terms obtained from the population, exposure, and outcome parameters of the research question. Primary research published in peer-reviewed journals and grey literature will be the source for the evidence map. Studies eligible for inclusion may involve: food crops and related research model plants exposed to SMF or non-ionizing EMF; treatment at all plant developmental stages excluding post-harvest improvement of food crops; and the presence of control groups. Eligible literature will be screened at the title, abstract, and full text levels. The validity of studies will not be critically appraised for the evidence map. A process of double extraction and coding of relevant information from eligible literature will be conducted. Within the evidence map, relevant data will be presented in the forms of text, graphs, tables, and figures. This will illustrate research trends, bring clarity to the evidence base concerning clusters of sufficient findings and areas of significant gaps, and inform stakeholders in decisions concerning research planning and policy formulation.
Infection prevention and control policies in hospitals and prevalence of highly resistant microorganisms: an international comparative study
Authors: van Dijk Manon D. , Voor in ’t holt Anne F. , Alp Emine , Hell Markus , Petrosillo Nicola , Presterl Elisabeth , Tsakris Athanasios , Severin Juliëtte A. , Vos Margreet C. ,   on behalf of the ESCMID Study Group for Nosocomial Infections (ESGNI)
Published: December 2022

Abstract
Background
There are differences in infection prevention and control (IPC) policies to prevent transmission of highly resistant microorganisms (HRMO). The aim of this study is to give an overview of the IPC policy of six European hospitals and their HRMO prevalence, to compare the IPC policies of these hospitals with international guidelines, and to investigate the hospitals’ adherence to their own IPC policy.

Methods
The participating hospitals were located in Salzburg (Austria), Vienna (Austria), Kayseri (Turkey), Piraeus (Greece), Rome (Italy) and Rotterdam (The Netherlands). Data were collected via an online survey. Questions were aimed at prevalence rates in the years 2014, 2015, 2016 of carbapenemase-producing Klebsiella pneumoniae (CPK), carbapenemase-producing Pseudomonas aeruginosa (CPPA), vancomycin-resistant Enterococcus faecium (VRE) and hospitals’ IPC policies of 2017. Implemented IPC measures (i.e. with a self-reported adherence of > 90%) were counted (26 points maximal).

Results
The self-reported prevalence of CPK per year was low in the Austrian and Dutch hospitals and high in the Turkish and Greek hospitals. CPPA was highly prevalent in the Turkish hospital only, while the prevalence of VRE in four hospitals, except the Austrian hospitals which reported lower prevalence numbers, was more evenly distributed. The Dutch hospital had implemented the most IPC measures (n = 21), the Turkish and Greek hospitals the least (n = 14 and 7, respectively).

Conclusion
Hospitals with the highest self-reported prevalence of CPK and CPPA reported the least implemented IPC measures. Also, hospitals with a higher prevalence often reported a lower adherence to own IPC policy.
Transport equipment network analysis: the value-added contribution
Authors: Hernández García Luis Gerardo 
Published: December 2022

AbstractEmerging in the twenty-first century, Network Science provides practical measures to interpret a system’s interactions between the components and their links. Literature has focused on countries’ interconnections on the final goods, but its application on the value-added from a network perspective in trade is still imitated. This paper applies network science properties and a multi-regional input–output analysis by using the UNCTAD-Eora Global Value Chain Database on the Transport Equipment value added on 2017 to unwrap the specific structural characteristics of the industry. Results show that the industry is highly centralized. The center of the network is dominated by developed countries, mainly from Europe, the United States, and Japan. Emerging countries such as China, Mexico, Thailand, and Poland also have an important position. In addition, the structure reveals two sub-hubs located in East Europe and North America. By extending to community detection, the network consists of three different communities led by Germany, the United States, and the United Kingdom, associated with more significant value-added flows. The study concludes that flows are not always consistent with the economy’s geographical location as usually final goods analysis suggests, and highlight the need to continue using the complex network to reveal the world trade structure.
Tadalafil increases the antitumor activity of 5-FU through inhibiting PRMT5-mediated glycolysis and cell proliferation in colorectal cancer
Authors: Shen Yao , Zhao Pan , Dong Kewei , Wang Jiajia , Li Huichen , Li Mengyang , Li Ruikai , Chen Suning , Shen Yuxia , Liu Zhiyu , Xie Mianjiao , Shen Peng , Zhang Jian 
Published: December 2022

Abstract
Background
Protein arginine methyltransferase 5 (PRMT5) is upregulated in multiple tumors and plays a pivotal role in cancer cell proliferation. However, the role of PRMT5 in colorectal cancer remains poorly understood.

Methods
We detected the expression level of PRMT5 and glycolytic enzymes using online databases and colorectal cancer cell lines by immunohistochemical staining, quantitative real-time polymerase chain reaction (qRT-PCR), and western blotting. And MTT and colony formation assays were conducted to investigate cell proliferation. Then, we evaluated ECAR and OCR levels using a biological energy analyzer to investigate the energy status of colorectal cancer, and the transcriptional regulation was detected by dual luciferase reporter assay and ChIP assay. Finally, the efficacy of combined treatment of tadalafil and 5-FU was verified.

Results
PRMT5 was highly expressed in colorectal cancer tissues compared with their normal counterparts and correlated with poor prognosis in CRC patients. Then, we demonstrated that PRMT5 knockdown or loss of function attenuated the viability of CRC cells, while overexpression of PRMT5 promoted cell proliferation. Mechanistically, PRMT5 enhanced glycolysis through transcriptionally activating LDHA expression. In addition, the PRMT5 inhibitor, tadalafil, rendered CRC cells sensitive to antitumor agent 5-FU in vitro and in vivo.

Conclusions
Our data indicates that PRMT5 promoted colorectal cancer proliferation partially through activating glycolysis and may be a potential target for colorectal cancer therapy.