bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium
Authors: Vickovic Sanja , Schapiro Denis , Carlberg Konstantin , Lötstedt Britta , Larsson Ludvig , Hildebrandt Franziska , Korotkova Marina , Hensvold Aase H. , Catrina Anca I. , Sorger Peter K. , Malmström Vivianne , Regev Aviv , Ståhl Patrik L. 
Published: February 2022

AbstractThe inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profiling after tissue homogenization. Here, we use Spatial Transcriptomics, where tissue-resident RNA is spatially labeled in situ with barcodes in a transcriptome-wide fashion, to study local tissue interactions at the site of chronic synovial inflammation. We report comprehensive spatial RNA-Seq data coupled to cell type-specific localization patterns at and around organized structures of infiltrating leukocyte cells in the synovium. Combining morphological features and high-throughput spatially resolved transcriptomics may be able to provide higher statistical power and more insights into monitoring disease severity and treatment-specific responses in seropositive and seronegative rheumatoid arthritis.
In vitro reconstitution of an efficient nucleotide excision repair system using mesophilic enzymes from Deinococcus radiodurans
Authors: Seck Anna , De Bonis Salvatore , Saint-Pierre Christine , Gasparutto Didier , Ravanat Jean-Luc , Timmins Joanna 
Published: February 2022

AbstractNucleotide excision repair (NER) is a universal and versatile DNA repair pathway, capable of removing a very wide range of lesions, including UV-induced pyrimidine dimers and bulky adducts. In bacteria, NER involves the sequential action of the UvrA, UvrB and UvrC proteins to release a short 12- or 13-nucleotide DNA fragment containing the damaged site. Although bacterial NER has been the focus of numerous studies over the past 40 years, a number of key questions remain unanswered regarding the mechanisms underlying DNA damage recognition by UvrA, the handoff to UvrB and the site-specific incision by UvrC. In the present study, we have successfully reconstituted in vitro a robust NER system using the UvrABC proteins from the radiation resistant bacterium, Deinococcus radiodurans. We have investigated the influence of various parameters, including temperature, salt, protein and ATP concentrations, protein purity and metal cations, on the dual incision by UvrABC, so as to find the optimal conditions for the efficient release of the short lesion-containing oligonucleotide. This newly developed assay relying on the use of an original, doubly-labelled DNA substrate has allowed us to probe the kinetics of repair on different DNA substrates and to determine the order and precise sites of incisions on the 5′ and 3′ sides of the lesion. This new assay thus constitutes a valuable tool to further decipher the NER pathway in bacteria.
Professional Guidelines and Reported Practice of Audiologists Performing Fall Risk Assessment With Older Adults: A Systematic Review
Authors: van Rie Kayla J. , Kanji Amisha , Naudé Alida 
Published: February 2022

Purpose:
This systematic review aimed to explore the recommended fall risk assessment practices in audiology, identify audiologists' reported practices in fall risk assessment, and recognize the barriers and facilitators affecting fall risk assessment in clinical practice.

Method:
This systematic review was conducted using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses.

Results:
CINAHL, PubMed, and gray literature yielded 262 articles. A total of 27 full-text articles were included in this review article. An additional 16 scope of practice and guideline documents were also reviewed. Pertinent data and findings from the review were tabulated and analyzed using a qualitative, inductive approach.

Conclusions:
Results revealed that despite fall risk assessment measures and protocols being mentioned, discussed, and reportedly implemented clinically in audiology literature, many audiologists are not conducting fall risk assessments clinically. The main challenges presented appear to be due to limited guidance within audiology documentation and inadequate training and knowledge of audiologists on fall risk factors and measures. This review article highlighted that all audiologists have an important role to play in reducing the global crisis of falls in older adults. However, without further research to aid in the development of standardization of documentation and training programs, we may continue to see a lack of awareness and education on fall risk and on the audiologist's role in the screening and early detection hereof.
Water content trends in K2-138 and other low-mass multi-planetary systems
Authors: Acuña L. , Lopez T. A. , Morel T. , Deleuil M. , Mousis O. , Aguichine A. , Marcq E. , Santerne A. 
Published: February 2022

Context. Both rocky super-Earths and volatile-rich sub-Neptunes have been found simultaneously in multi-planetary systems, suggesting that these systems are appropriate to study different composition and formation pathways within the same environment.
Aims. We perform a homogeneous interior structure analysis of five multi-planetary systems to explore compositional trends and their relation with planet formation. For one of these systems, K2-138, we present revised masses and stellar host chemical abundances to improve the constraints on the interior composition of its planets.
Methods. We conducted a line-by-line differential spectroscopic analysis on the stellar spectra of K2-138 to obtain its chemical abundances and the planetary parameters. We selected multi-planetary systems with five or more low-mass planets (M < 20 M⊕) that have both mass and radius data available. We carried out a homogeneous interior structure analysis on the planetary systems K2-138, TOI-178, Kepler-11, Kepler-102, and Kepler-80. We estimated the volatile mass fraction of the planets in these systems assuming a volatile layer constituted of water in steam and supercritical phases. Our interior-atmosphere model took the effects of irradiation on the surface conditions into account.
Results. K2-138 inner planets present an increasing volatile mass fraction with distance from their host star, while the outer planets present an approximately constant water content. This is similar to the trend observed in TRAPPIST-1 in a previous analysis with the same interior-atmosphere model. The Kepler-102 system could potentially present this trend. In all multi-planetary systems, the low volatile mass fraction of the inner planets could be due to atmospheric escape, while the higher volatile mass fraction of the outer planets can be the result of accretion of ice-rich material in the vicinity of the ice line with later inward migration. Kepler-102 and Kepler-80 present inner planets with high core mass fractions which could be due to mantle evaporation, impacts, or formation in the vicinity of rocklines.
L’histoire de l’expansion de l’Univers dévoilée
Authors: Burtin Étienne 
Published: February 2022

Depuis que les êtres humains regardent la voute céleste, ils essaient de trouver une explication aux motifs qu’ils y observent. Les différentes civilisations ont regroupé les étoiles visibles en constellations auxquelles elles ont donné des noms et ont attribué des vertus. Puis, elles ont compris que la Voie lactée qui barre le ciel rassemble une centaine de milliards d’étoiles en une galaxie dont notre étoile, le Soleil, et la Terre font partie.
Avec l’avènement des premiers télescopes, il a été établi que d’autres galaxies existaient au-delà de la Voie lactée, que leur nombre n’avait de limite que celle des instruments d’observation et qu’elles s’éloignent de nous d’autant plus vite qu’elles sont lointaines. Ainsi sont nés les « relevés de galaxies » dont les objectifs sont de dresser une carte de l’Univers pour améliorer la compréhension que l’on a de son évolution, et de déterminer la nature de ses constituants.
Update Breast Cancer 2021 Part 4 – Prevention and Early Stages
Authors: Thomssen Christoph , Fehm Tanja N. , Stickeler Elmar , Fasching Peter A. , Janni Wolfgang , Kolberg-Liedtke Cornelia , Kolberg Hans-Christian , Lüftner Diana , Müller Volkmar , Schütz Florian , Belleville Erik , Bader Simon , Untch Michael , Welslau Manfred , Thill Marc , Hartkopf Andreas D. , Tesch Hans , Ditsch Nina , Lux Michael P. , Wöckel Achim , Aktas Bahriye , Schneeweiss Andreas , Würstlein Rachel 
Published: February 2022

AbstractThis past year has seen new and effective options for further improving treatment outcome in many patients with early-stage breast cancer. Patients with hormone receptor-positive disease benefited significantly from the addition of the CDK4/6 inhibitor abemaciclib to endocrine adjuvant therapy. In triple-negative disease, data were presented for two treatment regimens. Patients with advanced disease (stage 2 and 3) benefit from neoadjuvant treatment with the immune checkpoint inhibitor pembrolizumab in combination with standard chemotherapy, regardless of PD-L1 expression. When neoadjuvant therapy has failed to achieve the desired remission in BRCA1 and BRCA2 mutations, the administration of the PARP inhibitor olaparib has demonstrated an impressive response. Other data address translational issues in HER2-positive breast cancer and neoadjuvant therapy approaches with the oral SERD giredestrant and the PARP inhibitor talazoparib. This review presents and analyses the findings of this yearʼ s most important study outcomes.
Effect of prophylactic endoscopic clipping for prevention of delayed bleeding after endoscopic papillectomy for ampullary neoplasm: a multicenter randomized trial
Authors: Park Se Woo , Song Tae Jun , Park Jin Seok , Jun Jae Hyuk , Park Tae Young , Oh Dong Wook , Lee Sang Soo , Kim Myung-Hwan 
Published: February 2022

Background Endoscopic clip placement is technically challenging using a duodenoscope, limiting their application for treatment of bleeding after endoscopic papillectomy. This study evaluated the efficacy of newly designed clips to prevent bleeding after endoscopic papillectomy.
Methods Patients (n = 80) with suspected benign adenomas on the major papilla who were scheduled for endoscopic papillectomy with or without clipping were randomized. A new duodenoscope-compatible clip capable of being rotated, reopened, and repeatedly repositioned was used. The primary end point was incidence of delayed bleeding.
Results The clipping procedure was successful in all patients. The incidence of delayed bleeding was nonsignificantly higher in the no-clipping group than in the clipping group (31.6 % [95 % confidence interval (CI) 19.1–47.5] vs. 15.0 % [95 %CI 7.1–29.1]). The incidence of post-endoscopic retrograde cholangiopancreatography pancreatitis did not differ significantly between the groups (clipping vs. no-clipping: 17.5 % [95 %CI 8.7–31.9] vs. 5.3 % [95 %CI 1.5–17.3]), and all cases were mild.
Conclusions Placement of the newly designed rotatable clip was technically feasible and tended to have a protective effect by preventing delayed bleeding after endoscopic papillectomy, although statistical significance was not reached.
Effect of Optic Canal Opening on Postoperative Visual Acuity in Patients with Tuberculum Sellae Meningiomas
Authors: Chen Lihua , Zhang Hongtian , Gao Mou , Chen Wenjin , Sun Kai , Xu Ruxiang 
Published: February 2022

Abstract
Background Tuberculum sellae meningiomas (TSMs) account for 5 to 10% of all intracranial meningiomas. They typically invade the optic canal and displace the optic nerve upward and laterally. The transcranial approach has been the standard surgical approach, while the transsphenoidal approach has been proposed for its minimally invasive nature; however, some reservations concerning this approach remain.
Methods From January 2000 to December 2018, a total of 97 patients who were diagnosed with TSM with invasion of the optic canal were enrolled and underwent microsurgery for tumor removal with optic canal opening. A retrospective analysis was performed on the effect of optic canal opening on postoperative visual acuity improvement. The median follow-up was 17.4 months (range: 3–86 months).
Results Among the 97 patients with TSM involving the optic canal, optic canal invasion was seen on preoperative imaging in 73 patients and during intraoperative exploration in all patients. In total, 87/97 patients (89.7%) underwent optic canal opening to remove tumors involving the optic canal, and the rate of total macroscopic resection of tumors invading the optic canal was 100%. Among the 10 patients who did not undergo optic canal opening, the rate of total resection of tumors involving the optic canal was 80% (8/10, p < 0.001). There were no deaths or serious complications. The postoperative visual acuity improvement rate was 64.4%, 23.7% maintained the preoperative level, and the visual acuity deteriorated 11.9%.
Conclusion Intraoperative optic canal opening is the key to total resection of TSMs involving the optic canal and improving postoperative visual acuity.
Comparison of the removal ability of basket catheters for small bile duct stones impacted in the corner pocket of the lower bile duct
Authors: Inoue Tadahisa , Ibusuki Mayu , Kitano Rena , Sakamoto Kazumasa , Kimoto Satoshi , Kobayashi Yuji , Sumida Yoshio , Nakade Yukiomi , Ito Kiyoaki , Yoneda Masashi 
Published: February 2022

Abstract
Background Endoscopic stone extraction is often challenging in patients with a significant lower bile duct corner pocket, where small stones are likely to escape and become impacted. This study aimed to determine the utility of different types of baskets for retrieving corner pocket stones.
Methods Seven types of retrieval baskets were evaluated on silicone models. The study outcomes included the characteristics of each basket and technical success rates associated with the retrieval of stones impacted in the corner pocket.
Results The overall success rate was only 27.5 %, with significant differences between the baskets (P < 0.001). Only three baskets yielded successful removal. The success rates were 10 %, 25 %, and 70 % with the 8-wire Nitinol Basket, RASEN, and VorticCatch V, respectively, all of which are helical eight-wire baskets. RASEN and VorticCatch V share similar characteristics, including a strong spiral wire and smaller interwire spaces at their distal ends. The addition of rotation significantly increased the success rate to 80 % with the 8-wire Nitinol Basket (P < 0.001) and 90 % with RASEN (P < 0.001).
Conclusions Robust helical eight-wire baskets with smaller interwire spaces at the tip may be effective for extracting corner pocket stones, while the addition of rotational motion may improve the efficacy of retrieval.
Polyamines effectively mitigate senescence in persistent leaves of
Authors: Altaf Foziya , Parveen Shazia , Farooq Sumira , ul Haq Aehsan , Lone Mohammad Lateef , Tahir Inayatullah , Kaushik Prashant , El-Serehy Hamed A. 
Published: February 2022

Plant leaves provide a unique insight into the changes that occur in organs, tissues and cells as they approach senescence. As part of the parental outlay, plants instigate leaf senescence to reallocate resources from older tissues to new organs towards the termination of the growing season. The aim of crop breeding initiatives is to optimize senescence for specific species. Considering hormonal regulation and their crosstalk during leaf senescence through integration of developmental signals, this work examines the efficacy of polyamines (PAs) in modulating several biochemical and physiological aspects with an ultimate aim to delay leaf senescence in leaf discs of Berginia ciliata (Haw.) sternb. Leaf discs were treated with putrescine (Put), spermidine (Spd) and spermine (Spm) at 20 μM, 20 μM and 15 μM concentration, respectively. A set of leaf discs kept in distilled water served as the control. Leaf discs treated with PAs were green and fresh by about 4 days compared to the control, thus exhibited delayed senescence. This delayed leaf senescence corroborated with the maintenance of high activity of reactive oxygen species (ROS) scavenging antioxidant enzymes viz, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and higher content of chlorophylls. A marked increase was also observed in membrane stability and soluble proteins in leaf discs treated with PAs. Exogenous PAs reduced oxidative stress in the leaf discs, as revealed by lower malondialdehyde (MDA) level, which is manifested as reduced lipid peroxidation (LPO). Improved membrane stability was proportional to lower LPO, as measured by the membrane stability index (MSI).
Reversible lysine fatty acylation of an anchoring protein mediates adipocyte adrenergic signaling
Authors: Bagchi Rushita A. , Robinson Emma L. , Hu Tianjing , Cao Ji , Hong Jun Young , Tharp Charles A. , Qasim Hanan , Gavin Kathleen M. , Pires da Silva Julie , Major Jennifer L. , McConnell Bradley K. , Seto Edward , Lin Hening , McKinsey Timothy A. 
Published: February 2022

Significance
Recently, histone deacetylase 11 (HDAC11) was shown to function as an enzyme that removes lipids such as myristoyl groups from lysines in proteins, yet only one substrate of HDAC11 has been reported. Here, we define gravin-α/A kinase–anchoring protein 12 as a second HDAC11 substrate. By demyristoylating gravin-α in adipocytes, HDAC11 prevents β-adrenergic receptors (β-ARs), which are G protein–coupled receptors (GPCRs), from translocating to membrane microdomains that are required for downstream protective signaling by protein kinase A (PKA). These findings demonstrate a role for reversible lysine myristoylation in the control of GPCR signaling and lay the foundation for developing therapeutics for obesity based on enhancing β-AR signaling in adipose tissue by manipulating the HDAC11:gravin-α axis.
‘If you see [blank], say [blank]’
Authors: Barrera-Vilert Maria 
Published: February 2022

Abstract
The September 11 terrorist attacks emerged as a turning point on security standards, contouring many aspects of
public life ever after. Two decades later, the see something, say something campaign stands as one of the New
York City trademarks. Its ubiquity across subway platforms intends to raise public awareness by transferring security
responsibilities to the general public.
Language is neither innocent nor merely instrumental, any more than is not neutral. This paper disentangles the
construction of something in the current context, where the elusive definition of terrorism has enabled distorted
perceptions of risk and certainty. The paper adopts a multimodal critical discourse analysis, focusing on the campaign’s use of
ambiguity and its lexical and semiotic choices. Ultimately, it intends to crystallize how language resonates with a broader
preemptive and never-ending War on Terror rhetoric while paving the way to further analyze the activation of the target of this
campaign: you.
The discursive construction of gay people in news reports of selected Nigerian newspapers
Authors: Adegbola Olubunmi Funmi 
Published: February 2022

AbstractSame-sex relationships have, over time, stirred serious debates worldwide. Studies on same-sex sexualities in the Nigerian context have focused on its representation in Nollywood movies and other arguments centred on ethics, culture and religion, with little attention paid to how queer people are framed by the Nigerian media. This study, therefore, explores agency and processes in the representation of gay people in news reports of selected Nigerian newspapers, in order to unearth how this social group is discursively constructed in the Nigerian context. Drawing on insights from Fairclough’s approach to critical discourse analysis and Halliday’s Systemic Functional Linguistics, this study considers three popular Nigerian newspapers (Vanguard, Nigerian TribuneandThe Punch) within three years (2013–2015, being the period of intense debate on the legalisation of the anti-gay bill in Nigeria). Results reveal that gay people are negatively evaluated as actors of negative material processes such as ‘murder’ and other violent actions, and goals of the actions of ‘arrests’ and ‘remands’, ideologically portraying them as criminals and dangerous. The study provides insight into the biased posturing of the Nigerian media on important social/national issues such as same-sex relationships.
Il congiuntivo alla danese
Authors: Andersen Anders , Strudsholm Erling 
Published: February 2022

Abstract
This article assesses the theory of ‘the subjunctive as a subordination marker’ proposed by Schmitt Jensen (1970a) in light of recent advances in the study of complementation. It argues that the
theory is consistent with the functional framework of Givón (2001) where event
integration is said to be mirrored in syntactic integration, but that it has to be revised on two points. Firstly, instead of
marking syntactic subordination, we propose that the subjunctive is used to prompt a non-referring construal of the subordinate
clause in certain constructions. Secondly, we suggest that the subjunctive prompts a conceptually secondary construal of the
subordinate clause in other constructions. By proposing a twofold function of the subjunctive, we can keep the main findings of
Schmitt Jensen while avoiding potential circularity and shortcomings that the syntactic theory of the subjunctive faces when
applied to, e.g., mood alternation.
A triboelectric nanosensor based on ultra-thin MXene composite paper for heavy metal ion detection
Authors: Wang Engui , Cao Yu , Bai Yuan , Gai Yansong , Shan Yizhu , Li Qi , Jiang Tao , Feng Hongqing , Li Zhou 
Published: February 2022

Abstract
Heavy metal pollution has become increasingly serious in recent decades with the progress of industrialization, posing a significant threat to human health. This raises the demand for portable and ease of use heavy metal ion detection devices. In this study, we develop ultra-thin (5 µm) and highly flexible composite paper of MXene/bacterial cellulose (M/BC
x
, with x denoting the BC content) and apply it in a self-powered triboelectric nanosensor (TENS) to do heavy metal ion detection. The M/BC
x
composite paper is fabricated using a simple vacuum filtration method, and combines the advantages of the high electrical conductivity of MXene with the excellent mechanical properties of BC. The TENS employs the M/BC
x
composite paper and polytetrafluoroethylene as the friction layers, and the influences of different ratios of M/BC
x
on the electrical signals is investigated. The TENS shows high sensitivity in the detection of Cu2+, Cr3+, and Zn2+, as the detection limit is as low as 1 µM without the need of ligand molecules. A linear range of 10–300 µM is obtained. The TENS also shows excellent stability after more than 10 000 continuous operations. This simple-structured, cost-effective and durable TENS device provides new insights into the methodology of heavy metal ion detection and can be further developed for the detection of the corresponding ions in serum.