bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hippocampal neuroinflammatory markers in intracerebroventricular streptozotocin injected rats are correlated with the memory impairments at different time points of post-injection.
Authors: Singh Nicky , Ghosh Rupsa , Bandopadhyay Debasish , Bandyopadhay Anupam , Ghosh Tusharkanti 
Published: December 2022

Abstract
Intracerebroventricular (ICV) injection of streptozotocin (STZ) induces memory impairments and neuroinflammation in rats but the relation between neuroinflammation and memory impairments in ICV-STZ injected rats is not well understood. In the present study the memory impairments [working memory errors (WME) and reference memory errors RME)] in ICV-STZ injected rats were assessed with concomitant changes in the neuroinflammatory markers (TNF α, IL-1β, COX2, PGE2) in hippocampus at five different time points after ICV-STZ injection (3rd hour to 21st day post injection). Results showed that the WME and RME were increased gradually with time, and the neuroinflammatory markers were also gradually increased concomitantly after ICV STZ injection. These higher levels of the inflammatory markers are correlated with the memory impairments (WME and RME) and probably indicate a link between memory impairments and neuroinflammation.
Initial outcomes of single-stage operation through modified posterior sagittal anoplasty approach for intermediate anorectal malformations in the neonate
Authors: Quynh Tran Anh , Liem Nguyen Thanh , Hien Pham Duy , Kien Hoang Huu , Du Le Quang , Long Le Hoang , Hung Tran , Lam Bui , Tran Nguyen Thi Ngoc , Duc Dinh Anh 
Published: December 2022

Abstract
Background:Three-stage posterior sagittal anorectoplasty (PSARP) is the standard technique for treated of high and intermediate anorectal malformations and is widely used worldwide. Single-stage modified PSARP approach for intermediate anorectal malformations has been reported. The study aims to describe the surgical technique and initial outcomes of single-stage operation through a modified PSARP approach preserving the external sphincter intact for intermediate anorectal malformations in neonates.
Patients and Method: A prospective cohort study was done on twenty-five patients with intermediate anorectal malformations operated on by single-stage operation through a modified PSARP approach preserving the external sphincter intact from May 2021 to June 2022. Variables included age of operation, associated anomalies, type of fistula, duration of hospital stay, complications, and initial functional outcomes.
Surgical technique: Skin incision behind the rectum, dissected and exposed the rectal pouch, preserved the sphincter complex intact and dissected the rectal pouch from the posterior wall of the urethra or vagina. Suture the urethral fistula (if any). Use muscle stimulation to define the sphincter center, make a tunnel through the sphincter center, and the rectum through the sphincter complex center for anorectoplasty.
Results: 25 patients including 22 boys and 3 girls, the mean age at operation were 25.3 ± 6.0 hours (range, 20 - 48 hours). The mean operative time was 49.4 ± 9.9 minutes (range 30–75 minutes), and the mean hospital stay was 6.7 ± 1.2 days (from 5 to 9 days). There were no early postoperative complications. Follow-up from 2 to 15 months, all patients have 1-6 bowel movements/day. There were 2 patients with rectal pouch syndrome and redo-operated colon pull-through by laparoscopy. Rectal mucosal prolapse occurred in 3 patients, No patient has anal stenosis or constipation.
Conclusion: Single-stage operation through a modified PSARP approach preserving the external sphincter intactfor intermediate anorectal malformations in the neonate is feasible, safe, and effective.
HA/PEEK Scaffolds with Modified Crystallinity via 3D-bioprinting for Multiple Applications in Hard Tissue Engineering
Authors: Chen Jiahao , Xiao Jingyi , Han Xue , Sima Xiutian , Guo Weihua 
Published: December 2022

Abstract
Background
Hard tissues, especially teeth and bones, are highly mineralized and the large-scale defect or total loss of them is irreversible. Scaffolds fabricated with polyether ether ketone (PEEK) and hydroxyapatite (HA) were considered as substitute materials with osteogenic properties but poor mechanical properties. There is a lack of a construction strategy of HA/PEEK scaffolds that can balance mechanical and biological properties and the in vitro and in vivo evaluation of them.
Methods
Based on fused deposition melting (FDM), we regulated the crystallinity and mixing ratio of HA of HA/PEEK scaffolds, and explored the material characterization including crystallinity, element content and mechanical properties and biological properties including cell proliferation, migration, osteogenic differentiation of different scaffolds. And we further mimicked the clinical application on the Beagles by implanting the scaffolds to reconstruct the defect of teeth, mandibles and ribs.
Results
We found that the 3D-20%HA/PEEK scaffolds with low crystallinity achieved the required strength and elasticity, and exhibited the characteristics of promoting the proliferation, migration and osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). The results of the implantation of Beagles’ teeth, mandible and rib showed that the 3D-20%HA/PEEK scaffold with low crystallinity could well withstand the local complex force in the defect area and combine well with natural bone tissue.
Conclusion
The HA/PEEK scaffolds constructed with this strategy had appropriate mechanical properties to withstand complex forces, and good bone regeneration effect, which made them practical and versatile on hard tissue engineering.
Application of Cloud Computing in Healthcare – A Comprehensive Study
Authors: Bose Anindya , Roy Sandip , Bose Rajesh 
Published: December 2022

Abstract
A new approach to successfully offering IT services is provided by cloud computing. The body of research implies that cloud technology can raise the quality of services across a range of sectors, especially healthcare. Before being widely used, cloud computing should indeed be thoroughly assessed, like any other technical advance. Many researchers and clinicians are interested in the availability of large, quickly available, and reconfigurable resources including virtual systems, platforms, and applications with minimal service costs. This study gives a thorough analysis of academic literature on cloud computing in the healthcare industry. To propose a categorization methodology with five different categories — Cloud-based framework development in healthcare, virtual machine placement in healthcare, cloud-based Green healthcare, security in cloud-based healthcare, and broker development in healthcare. The authors reviewed 227 articles before narrowing them down to 40. At the end some future research prospects have been highlighted.
Taxonomy, biological characterization and fungicide sensitivity assays of Hypomyces cornea sp. nov. causing cobweb disease on Auricularia cornea
Authors: Xie Jiangtao , Lu Sibei , Pan Yintao , Peng Keqin , Zeng Xiangyu , Tian Fenghua 
Published: December 2022

Abstract
Hypomyces spp. is an important group of Hypocreaceae that are widely distributed all over the world, one of the main pathogenic of mushroom cobweb disease. In July 2021, three strains were isolated from symptoms of cobweb disease on fruiting bodies of Auricularia cornea in Guizhou Province, China. Based on phylogenetic analysis and morphological studies, they were then introduced as a new taxon, Hypomyces cornea sp. nov. The pathogenicity test showed that the mushrooms infected with spore suspension had the same symptoms as those in the field, and the same pathogens could be re-isolated, which fulfill Koch’s postulates. The biological characterization of Hypomyces cornea was explored to identify conditions that may be conducive to pathogen habitat, including the effects of different temperature, pH, carbon source, nitrogen source and light conditions. The results showed that the optimal temperature for the pathogen is 25℃, pH = 6, and the best carbon and nitrogen source are soluble starch and peptone, respectively. Nine common fungicides were screened in vitro by hyphal growth inhibition method, and the most suitable fungicide is Prochloraz manganese chloride complex, with high inhibition rate to the pathoge and low inhibition rate to the mushrooms. The results will provide an important basis and material for effective prevention and control of Auricularia cornea cobweb disease.
Sensitivity of mid-ocean ridge hydrothermal system controlled by the detachment fault to the glacial cycle
Authors: Yang Xianhui , Tao Chunhui , liao Shili , Barriga Fernando , Deng Xianming , Liang Jin , Guo Zhikui , Wang Mingxu , Yang Weifang 
Published: December 2022

AbstractChanges in sea level caused by glacial cycles may influence the magmatism and hydrothermal activity of oceanic ridges. Recent studies showed that the response time of the hydrothermal activity in the intermediate-fast spreading ridges differs from that in the slow-spreading ridges to the glacial cycles, and a unified model is expected to explain it. Here, we report the 160 ka sediment record adjacent to the Yuhuang hydrothermal field on the Southwest Indian Ridge. Hydrothermal and detachment fault activities were found to enhance or weaken during glacial and interglacial periods, respectively. The magmatism of slow/ultraslow spreading ridges is more sensitive to sea level changes; with the synchronous effect of detachment faults, the hydrothermal activity responds faster to the glacial cycles. We established a model of Sea level change–Magmatism–Detachment fault activity–Hydrothermal activity to explain the different responses of the hydrothermal activity of the mid-ocean ridges to the glacial cycles.
Forecasting Pollutant Concentration for Water Resource Planning and Management, Using Water Quality Analyzer (WQA)
Authors: Jamil Nor Rohaizah , Bello Nura , Keng Yap Ng , Looi Ley Juen 
Published: December 2022

Abstract
An accurate estimation of present and future concentration of pollutant entering the water body provides data support and scientific basis for government decision-making and water resource management. This study conduct trend analysis of pollutant concentration using Water Quality Analyser (WQA), with a view to providing a scientific basis for decision making towards the implementation of the Malaysian Vision Valley (MVV) Development Plan, and other water resource development plans across the world, using river Linggi, Malaysia, as case study. The result indicated that the Water Quality class (WQ-class) of the river for respective pollutants would remain the same beyond the year 2030. Therefore, the proposed project is sustainable under present water management action. Hence, WQA is useful for water quality predictions for water resource planning and management.
High-throughput proteomics identifies THEMIS2 as independent biomarker of treatment-free survival in untreated CLL
Authors: Hengeveld Paul , Kolijn Pieter Martijn , Demmers Jeroen , Doff Wouter , Dubois Julie , Rijken Melissa , Assmann Jorn , Straten Lina van der , Boiten Henk Jan , Gussinklo Kirsten , Valk Peter , Faber Laura , Westerweel Peter , Kater Arnon , Levin Mark-David , Langerak Anton 
Published: December 2022

Abstract
It remains challenging in chronic lymphocytic leukemia (CLL) to distinguish between patients with favorable and unfavorable time to first treatment (TTFT). Additionally, the downstream protein correlates of well-known molecular features of CLL are not always clear. To address this, we selected 40 CLL patients with TTFT ≤ 24 months and compared their B cell intracellular protein expression with 40 age- and sex-matched CLL patients with TTFT > 24 months using mass spectrometry. In total, 3268 proteins were quantified in the cohort. IGHV mutational status and trisomy 12 were most impactful on the CLL proteome. Comparing cases to controls, five proteins were significantly upregulated, whereas three proteins where significantly downregulated. Of these, only THEMIS2, a signaling protein acting downstream of the B cell receptor, was significantly associated with TTFT, independently of IGHV and TP53 mutational status (HR 2.49, [95%CI 1.62–3.84], P < 0.001). This association was validated on the mRNA and protein level by qPCR and ELISA, respectively. Analysis of two independently generated RNA sequencing and mass spectrometry datasets confirmed the association between THEMIS2 expression and clinical outcome. In conclusion, we present a comprehensive characterization of the proteome of untreated CLL and identify THEMIS2 expression as a putative biomarker of TTFT.
Knowledge, perception, and desire for pain relief in labor among Iranian pregnant women
Authors: Moradi Fereshteh , Hashemian Morteza , Hosseini Fatemeh Sadat , Shafiei Shohreh , Alidousti Katayoun 
Published: December 2022

Abstract
Background: One way to promote vaginal delivery and reduce unnecessary cesarean section, is by paying attention to labor pain relief techniques. The aim was to investigate knowledge, perceptions, and desire for pain relief in labor among Iranian pregnant women
Methods: This descriptive study was conducted on 325 pregnant women who referred to prenatal clinics in 2020 and met the inclusion criteria. Data were collected using a researcher-made questionnaire on painless delivery. Data analysis was performed using SPSS v.21.
Results: Most of the respondents (74%) were between 20-29 years old. 19.69% of participants were nulliparous and 62.76% had diplomas.72% were housewives. 56.61% of the participants had not enough information about pain relief in labor. Only 14 people of whom had information, (9.92%) desired to experience pain relief in labor. Many of the respondents 90.76% were worried about the effects of pain relief on their unborn. 44.68% of participants, who had information about pain relief in labor; got their information regarding pain relief in labor through Internet sources.
Conclusions:Due to the low level of awareness of pregnant women to use painless delivery and labor pain relief techniques, it is recommended that the necessary measures be taken in order to increase women’s knowledge of the benefits and limitations of painless delivery.
BNT162b2 or CoronaVac as the Third Dose against Omicron: Neutralizing Antibody Responses Among Transplant Recipients Who Had Received Two Doses of CoronaVac
Authors: Erol Çiğdem , Kuloğlu Zeynep Ece , Kayaaslan Bircan , Esken Gülen , Altınsoy Adalet , Barlas Tayfun , Çınar Güle , Hasanoğlu İmran , Oruç Ebru , İncir Said , Azap Alpay , Korkmaz Gülten , Gökçe Dilara Turan , Kırımker Onur Elvan , Yenigün Ezgi Coşkun , Ölçücüoğlu Erkan , Soy Ebru Ayvazoğlu , Çetinkünar Süleyman , Azap Özlem Kurt , Can Füsun , Haberal Mehmet 
Published: December 2022

Abstract
Introduction: We evaluated neutralizing antibody and anti-spike antibody (anti-S) response against omicron variant in solid organ (SOT) or hematopoietic stem cell (HSTC) receivers after third dose of BNT162b2 (BNT) or CoronaVac (CV) following two doses of CV.
Methods: In total, 95 participants who underwent SOT (n=62; 44 liver, 18 kidney) or HSCT (n=27; 5 allogeneic, 22 autologous) were included from five centers in Turkey. The median time between third doses and serum sampling was 154 days. The vaccine-induced antibody responses of both neutralizing antibodies and Anti-Spike antibodies were assessed by plaque neutralizing assay and immunoassay.
Results: Neutralizing antibody and anti-spike IgG levels were significantly higher in transplant patients receiving BNT compared to those receiving CV (GMT:26.76 vs 10.89; p=0.03 and 2116 Au/ml vs 172.1 Au/ml; p<0.001). Solid organ transplantation recipients, particularly liver transplant recipients, showed lower antibody levels than HSCT recipients. Thus, among HSCT recipients, the GMT after BNT was 91.29 and it was 15.81 in the SOT group (p<0.001). In SOT, antibody levels after BNT in kidney transplantation recipients was significantly higher than that in liver transplantation recipients (GMT:48.32 vs 11.72) (p<0.001). Besides, the neutralizing antibody levels after CV were very low (GMT: 10.81) in kidney transplantation recipients and below the detection limit (<10) in liver transplant recipients. There was a weak correlation between the neutralizing and anti-Spike antibody responses (r=0.36).
Conclusion:
This study highlights the superiority of BNT responses against omicron as a third dose among transplant recipients after two doses of CV. Lack of neutralizing antibody against omicron after CV in liver transplant recipients should be taken into consideration particularly in countries where inactivated vaccines are available in addition to mRNA vaccines.
A case of nasal extranodal NK/T-cell lymphoma of lower limb muscle revealed by 18F-FDG PET/CT
Authors: Hu Xianwen , Li Wenxin , Li Dandan , Wang Pan , Cai Jiong 
Published: December 2022

Abstract
Background: Extranodal NK/T-cell lymphoma (ENKTCL) is a type of malignant non-Hodgkin’s lymphoma originating from mature T cells and NK cells, mainly involving the upper aerodigestive tract including the nasal cavity, nasopharynx, oropharynx, oral cavity, hypopharynx, larynx, and occasionally in the skin, salivary glands, testes, and gastrointestinal tract, but rarely in skeletal muscle.
Case presentation: An 82-year-old man presented with redness, swelling, and pain in his right lower limb for 3 months. He was initially diagnosed as cellulitis at another hospital, and was treated conservatively for two weeks without improvement. He underwent biopsy of the lesioned muscle and histopathology revealed nasal type ENKTCL. 18F-FDG PET/CT was recommended for the staging of the lymphoma, and the results showed that except for the muscles of the right lower extremity, no other organs and tissues were involved.
Conclusion: ENKTCL confined to the muscle of the lower extremity is rare and often initially misdiagnosed as myositis because of red, swollen, heat, and painful symptoms that resemble inflammation, of which higher radioactive uptake in 18F-FDG PET/CT helps to distinguish it from myositis.
Effect of the Blended Workshop Learning and Web-based Learning Sequence on the Learning Level: A new Experiment
Authors: Paknia Babak , Rafiei Mohammad , Khorsandi Mahboobeh , ramezani ghobad 
Published: December 2022

Abstract
Background and Objective: The blended electronic learning system, raised as a new approach in educational planning with great enthusiasm, seeks novel ways to properly combine the media; however, the sequence of in-person and distance educational methods is an issue neglected so far. This study aims to compare the effect of the sequence of blended workshop learning and web-based learning on the learning level of biostatistics in students who were members of the Student Research Committee at Arak Medical Sciences University.
Methodology: This is a quasi-experimental study with an alternative treatment design. The statistical population consisted of the students who were members of the Student Research Committee of the university, including 38 medical students and 15 health students, who were selected through census sampling due to their limited number. The data were selected with 2 questionnaires: demographic characteristics and biostatistics multiple-choice questions to assess learning of statistical concepts in three levels of literacy, reasoning, and thinking. The validity and reliability of the translated statistics questions were assessed through content validity and bisection, respectively. The data were analyzed in SPSS-16 through independent and paired t-tests and analysis of variance.
Findings: The results showed a statistical difference between the groups in both faculties in statistical literacy and reasoning in blended learning (p>0.05). However, regarding statistical thinking, the difference was significant in the health faculty (p=0.044) and insignificant in the medical school (p>0.05).
Discussion: Given the statistical difference observed in the health group, we suggest holding in-person courses at first and online courses then.
Ultra-thin 2D bimetallic MOF nanosheets for highly sensitive and stable detection of glucose in sweat for dancers
Authors: Mao Yufei , Chen Tangchun , Hu Yifan , Son Gack(Kwan)Jung 
Published: December 2022

Abstract
The measurement of glucose concentration in sweat is expected to replace the existing blood glucose detection, which realize the effective way of non-invasive monitoring of human glucose concentration during human sports. High precision glucose detection can be achieved by adjusting the electrode material of the sensor. Thus, in this work, the bimetallic organic frameworks (bi-MOFs) materials containing Mn and Ni ions (NiMn-MOF) with ultrathin nanosheets have been exquisitely designed. The ultrathin nanosheet and heterogeneous metal ions in the structure optimize the electronic structure, which improvs the electrical conductivity of MOFs. The success of the preparation strategy leads the good electrocatalytic performance of NiMn-MOF for glucose detection. Detailly, NiMn-MOF shows high sensitivity of 1576 μA mM-1 cm-2 in the linear rang from 0 to 0.205 mM and the wide linear region of 0.255-2.655 mM and 3.655-5.655 mM were also observe. In addition, the high repeatability, reproductivity, long-term stability and ultra-low limited of detection (LOD, 0.28 μM, S/N=3) provide foundation for the practical sensor application of this NiMn-MOF nanosheets. Remarkably, as designed NiMn-MOF sensor can accurately measure glucose concentration in sweat showing great potential in the field of wearable glucose monitoring during dancing.
Association of Blood Lipid Profile Components with White Matter Hyperintensity Burden in Cerebral Small Vessel Disease
Authors: Li Shanshan , Wang Yunchao , Yu Lulu , Gao Yuan , Yang Yinghao , Zhu Hanghang , An Lu , Yuan Wenxin , Wu Jinghao , Zong Ce , Xu Yuming , Li Yusheng 
Published: December 2022

Abstract
Background: Cerebral small vessel disease (CSVD) refers to a common cerebrovascular disease and white matter hyperintensities (WMHs) constitute a typical feature of CSVD. However, there has not been a large number of studies investigating the relationship between lipid profile components and WMHs.
Methods: Altogether, 1019 patients with CSVD were enrolled to the First Affiliated Hospital of Zhengzhou University between April 2016 to December 2021. Baseline data were collected for all patients, including demographic characteristics and clinical data. WMH volumes were evaluated by two experienced neurologists using the MRIcro software. Multivariate regression analysis was used to investigate the relationship among the severity of WMHs, blood lipids and common risk factors.
Results: Altogether, 1019 patients with CSVD were enrolled, including 255 in the severe WMH group and 764 in the mild WMH group. After including age, sex and blood lipids to construct a multivariate logistic regression equation, we observed that the severity of WMHs was independently predicted by low-density lipoprotein (LDL), the ratio of LDL to high-density lipoprotein (LDL/HDL), homocysteine level, and history of cerebral infarction.
Conclusion: We used WMH volume, a highly accurate measure, to assess its relationship with lipid profiles. The WMH volume increased with a decrease in LDL and LDL/HDL. This relationship was more significant especially among the subgroups of patients aged <70 years and men. Patients with cerebral infarction and higher homocysteine levels were more likely to have higher WMH volumes. Our study has provided a reference for clinical diagnosis and therapy, especially for discussing the role of blood lipid profiles in the pathophysiology of CSVD.