bluuebunny/crossref_metadata_2025_split huggingface dataset:
The spatio-temporal landscape of the transcriptome and metabolome of cotton fiber cells during their initiation and late development
Authors: Sun Xuwu , Qin Aizhi , Wang Xingxing , Ge Xiaoyang , Liu Zhixin , Guo Chenxi , Yu Xiaole , Zhang Xianliang , Lu Yao , Yang Jincheng , He Jiuming , Zhou Yaping , Hu Mengke , Liu Hao , Zhao Zihao , Hu Guanjing , Li Wei , Zang Xinshan , Dai Shuai , Sun Susu , Tong-Villalobos Lenin , Herrera-Estrella Luis , Tran Lam-Son Phan , Ma Xiongfeng 
Published: September 2022

Abstract

Cotton fibers develop from epidermal cells in the outer integument of ovules. The regulatory process underlying fiber cell development has been extensively studied, little is known about the spatio-temporal profiles of transcriptomes and metabolomes during its development. Here we characterized the dynamics of transcriptome and metabolome during the early developmental stages of cotton fiber cells using a combination of spatial transcriptomic, single-cell transcriptomic, and spatial metabolomic analyses. We identified the key genetic regulators and metabolites that initiate and determine the fate of fiber cells. Both knockdown and gain-of-function analyses of one new identified marker gene BEE3/Gh_A09G062900revealed its important role in the initiation of cotton fiber. We also designed a website for the public visualization of spatial gene expression in cotton that provides important reference datasets of spatio-temporal gene expression and can be used to further decipher the genetic and metabolic regulation of cotton fiber development (http://CottonOvule.cricaas.com.cn/).
Binge Eating Disorder: an Online Multidisciplinary Approach in Its Treatment
Authors: Valdez-Aguilar Mariana , Vázquez-Arévalo Rosalia , López-Aguilar Xóchitl , Martínez Ana Olivia Ruíz , Rosinska Magda , Mancilla-Díaz Juan Manuel 
Published: September 2022

Abstract
Background: Binge eating disorder (BED) is the most frequent loss of eating behavior among the population and the one that has received the least study in its treatment. Many studies on interventions and BED treatments have been carried out in the United States and Europe, few have been reported in Latin American populations. People with this disorder not only have physical consequences but also social and psychological ones, so a multidisciplinary treatment is a good alternative to treat this condition.
Objective: To evaluate the effectiveness of a multidisciplinary online treatment (psychological, nutritional, and physical activity) in patients with Binge Eating Disorder (BED).
Method: 15 Fifteen people diagnosed with BED (2 men and 13 women) with a mean age of 34.93 years (SD=11.91) and a mean initial BMI of 42 participated in this study. The participants were divided into two groups according to the severity of the disorder. The treatment was carried out over 28 sessions, each being two hours per week consisting of 1 hour of group therapy and 1 hour of individual therapy. There were four evaluations: pre, post and two follow-ups.
Results: Five patients did not complete the treatment (30%). The comparisons were made through the non-parametric Friedman test, finding a statistically significant decrease in binge eating symptoms (x2=15.57; p=.001), anxiety symptoms (x2=15.96; p=.001) and depression (x2=15.03; p=.002). There was an improvement in clarity (x2=11.60; p=.010) and emotional regulation (x2=7.75; p=.050), only in women. The patients reduced their body weight, andd improved their eating and exercise habits by introducing fruits and vegetables and including 20-30 minutes of physical activity into their daily routine. Regarding the Objective Clinical Change Index (OCC) From the objective clinical change, a positive change was observed in all the variables addressed.
Conclusions: The data presented allowed us to conclude that the online multidisciplinary intervention was effective in the treatment of BED.
Trial registration:Retrospectively registered
Comparison of safety and efficacy between Janus kinase inhibitors and abatacept for rheumatoid arthritis-associated interstitial lung disease
Authors: Tsujii Atsuko , Isoda Kentaro , Yoshimura Maiko , Nakabayashi Akihiko , Kim Dong-seop , Tamada Tatsuya , Yamamoto Kurumi , Ohshima Shiro 
Published: September 2022

AbstractBackground: Interstitial lung disease (ILD) related to Rheumatoid Arthritis (RA) is among the leading causes of death and an essential prognostic factor. There is only limited evidence for the safety of anti-rheumatic drugs for patients with RA-ILD. The aim of this study is to investigate the safety and efficacy of Janus kinase inhibitors (JAKis) by comparing it with abatacept (ABT) in patients with RA-ILD.Methods: This single center, retrospective nested case–control study enrolled patients with RA-ILD treated with JAKi or ABT. To determine the safety of the two drugs for existing ILD, we compared their drug persistency and incidence rates of pulmonary complications. For their efficacy as RA treatment, disease activity scores and prednisolone (PSL)-sparing effect were compared. We performed propensity score matching to match the groups’ patient characteristics.Results: We studied 71 patients with RA-ILD (ABT, n = 45; JAKi, n = 26). At baseline, the JAKi group had longer disease duration, longer duration of past bDMARD or JAKi use and higher usual interstitial pneumonia rate. After propensity score matching, no significant differences in patient characteristics were found between the two groups. No significant difference in the drug persistency rate for the first 2 years (ABT, 61.9%; JAKi, 42.8%;P =0.256) were observed between the two matched groups. The incidence rate of pulmonary complications did not differ significantly between the two groups (P =0.683). Regarding the efficacy for RA, a significant decrease in disease activity scores after the 1-year treatment was observed in both groups, and PSL dose was successfully tapered, although no significant differences were observed between the two drugs.Conclusions: JAKi is as safe and effective as ABT for patients with RA-ILD. JAKi can be a good treatment option for such patients.
Double-slot Ultra-compact Polarization Beam Splitter Based on Asymmetric Hybrid Plasmonic Structure
Authors: Wang Fang , Liu Hua , Ma TAO , Ma Shou Dao , Liu Yu Fang 
Published: September 2022

Abstract
To improve the extinction ratio of a polarization beam splitter, we propose a dual-slot ultra-compact polarization splitter (PBS) consisting of a hybrid plasma horizontal slot waveguide (HSW) and silicon nitride hybrid vertical slot waveguide (VSW). At the same time, the coating material is silicon dioxide, which can not only prevent the oxidation of the mixed plasma but also facilitate the integration with other devices. The mode characteristics of the HSW and VSW are simulated by using the finite element method (FEM). At suitable HSW and VSW widths, the TE polarization modes in HSW and VSW are phase-matched, while the TM polarization modes are phase mismatched. Therefore, the TE mode in HSW waveguide is strongly coupled with VSW waveguide by adopting a dual-slot, while the TM mode directly passes through the HSW waveguide. The results show that PBS achieves an extinction ratio (ER) of 35.1 dB and insertion loss (IL) of 0.34 dB for TE mode at 1.55 µm, For TM mode, PBS reached 40.9 dB for ER and 2.65 dB for IL. The proposed PBS is designed with 100 nm bandwidth, high ER, and low IL, which can be suitable for photonic integrated circuits (PICs).
Transaction Prices of German Closed-End Real Estate Funds in the Secondary Market and Their Influencing Factors
Authors: Aryoubi Abdullah , Bossauer Eduard 
Published: September 2022

Abstract

This study is the first to assess and contribute to a better understanding of the factors affecting the prices of closed-end real estate funds (CEREFs) in Germany’s secondary market. The dataset is based on 16,718 observations of realized transaction prices from 412 different German CEREFs; the factors comprise macroeconomic, fund, property, and liquidity variables. We regress macroeconomic factors on benchmark variables, such as listed real estate assets and the stock market, to observe whether German CEREFs react differently from the overall economic state from a portfolio diversification perspective. The benchmark variables are homogeneously driven by stock market excess return, DAX volatility index (VDAX) growth, and, to a certain extent, the bond rate, while CEREFs are mainly affected by the unemployment rate and economic activity. Fund and property age have a significant impact on CEREFs. Additionally, they have optimal levels at which their respective impacts start to reverse. Initial fund leverage and rising liquidity in the secondary market also have a significant impact. However, the observed effects vary across the types of real estate held by CEREFs, and investors must consider this in their pricing policies. Finally, this study presents a comprehensive overview of the German secondary market environment for CEREFs and examines their development.
Positron emission tomography imaging of the sodium iodide symporter senses real-time energy stress in vivo
Authors: Dzien Piotr , Mackintosh Agata , Malviya Gaurav , Johnson Emma , Soloviev Dmitry , Brown Gavin , Uribe Alejandro Huerta , Nixon Colin , Lyons Scott K. , Maddocks Oliver , Blyth Karen , Lewis David Y. 
Published: September 2022

Abstract
Background: Tissue environment is critical in determining tumour metabolic vulnerability. However, in vivo drug testing is slow and waiting for tumour growth delay may not be the most appropriate endpoint for metabolic treatments. An in vivo method for measuring energy stress would rapidly determine tumour targeting in a physiologically relevant environment. The sodium-iodide symporter (NIS) is an imaging reporter gene whose protein product co-transports sodium and iodide, and positron emission tomography (PET) radiolabelled anions into the cell. Here, we show that PET imaging of NIS-mediated radiotracer uptake can rapidly visualise tumour energy stress within minutes following in vivo treatment.
Methods: We modified HEK293T human embryonic kidney cells and A549 lung cancer cells to express transgenic. We treated cells and implanted tumours with drugs that caused metabolic stress to determine the effects in NIS activity and energy charge. We used [18F]tetrafluoroborate positron emission tomography (PET) imaging to non-invasively image NIS activity in vivo.
Results: NIS activity was ablated by treating HEK293T cells in vitro, with the Na+/K+ ATPase inhibitor digoxin, confirming that radiotracer uptake was dependent on the sodium-potassium concentration gradient. NIS-mediated radiotracer uptake was significantly reduced (-58.2%) following disruptions to ATP re-synthesis by combined glycolysis and oxidative phosphorylation inhibition in HEK293T cells and by oxidative phosphorylation inhibition (16.6%) in A549 cells in vitro. PET signal was significantly decreased (-56.5%) within 90 minutes from the onset of treatment with IACS-010759, an oxidative phosphorylation inhibitor, in subcutaneous transgenic A549 tumours in vivo, showing that NIS could rapidly and sensitively detect energy stress non-invasively, before more widespread changes to phosphorylated AMP-activated protein kinase, phosphorylated pyruvate dehydrogenase and GLUT1 were detectable.
Conclusions: NIS acts as a rapid metabolic sensor for drugs that lead to ATP depletion. PET imaging of NIS could facilitate in vivo testing of treatments targeting energetic pathways, determine drug potency and expedite metabolic drug development.
Spatially-explicit water quality responses to freshwater discharge in a tropical estuary, Hilo Bay, Hawai‘i
Authors: Lucas Sydney N. , Fouad Geoffrey , Adolf Jason E. 
Published: September 2022

Abstract
Hilo Bay, Hawai’i is an estuary of great importance to its neighboring coastal community, but is threatened by impaired water quality indicated by excessive turbidity and chlorophyll a associated with river discharges of sediments and nutrients. The Wailuku River in the western half of the bay is the primary source of freshwater discharge, hypothesized here to form a surface-water dominant half of the bay with different water quality traits than the groundwater dominant, eastern half of the bay where the spring-fed Wailoa River discharges. The water quality of both halves of the bay over different flow conditions of the Wailuku River is examined in this study using spatially explicit water quality sampling which collects hundreds of samples in either half of the bay at about every 40 meters. The dense sample shows significant differences between the two halves of the bay, with greater salinity dilution and turbidity in the surface-water dominant area. Both salinity and turbidity have a predictable relation to discharge, with salinity decreasing and turbidity increasing in higher flow conditions. Chlorophyll a, however, has a more complex relation to discharge, as chlorophyll a concentrations are greatest in high-flow conditions, but this may be confounded by the different seasons of the water quality samples. Furthermore, significantly greater chlorophyll a concentrations in the groundwater dominant half of the bay in low-flow conditions shows that discharge may be spuriously correlated to chlorophyll a, and further studies of the effects of surface-water discharge on chlorophyll a concentrations are warranted.
Kearifan Lokal dalam Mitigasi Bencana Gunung Api di Padukuhan Pangukrejo Pasca Erupsi 2010
Authors: Puspita Dewi  , Winarna  
Published: September 2022

Title: Local Wisdom in Volcanic Disaster Mitigation in Pangukrejo Village Post 2010 Eruption

Merapi, as one of the active volcanoes in Indonesia, become a threat to the surrounding settlements. Especially large eruption that happened in the 2010 which created crater openings that increased the potential for eruptions leading to Pangukrejo Village, as one of it's area. On the other hand, Pangukrejo Village included in the Disaster-Prone Area III (KRB III) but refused to be relocated. After the 2010 eruption, people realized that they need not only local wisdom but also mitigation efforts by government. Until now, both local wisdom and disaster mitigation have worked together to increasing the preparedness of the people in this village to face future eruptions of Merapi. This study aims to identify local wisdom that still being carried out and worked together with disaster mitigation in Pangukrejo Village, which reviewed from the physical aspects, such as zone planning, early warning systems, and building design. The research use descriptive qualitative method with data analysis using content analysis technique and choosing respondents using purposive sampling technique. Based on the results of the research, it was finally found that there are the same meanings and interrelated key patterns that make local wisdom still being carried out and worked together with disaster mitigation.
FAM83G-based Peptide Induces Apoptosis on Cultured Liver Cancer Cell
Authors: Okada Junichi , Yamada Eijiro , Saito Tsugumichi , Okada Kazuya , Yamada Masanobu , Ohshima Kihachi , Okada Shuichi 
Published: September 2022

Background:
Previously, AF-956, which contains S356 of FAM83G and an N-terminal
antenna peptide for entry into colon cancer cells, is markedly antiproliferative compared to a control
peptide (AF-859), which lacks the N-terminal antenna peptide, by inducing apoptosis via the
inhibition of HSP27 phosphorylation at residues S15 and S82.

Objective:
Because FAM83G-derived peptides are promising lead compounds for colon cancer
treatment, we reanalyzed the effect of AG-066, which contains S356 of FAM83G and an N-terminal
antenna peptide for entry into the liver cancer cells.

Methods:
HepG2 liver cancer cells were incubated with either AF-859 or AG-066 at a concentration
of 54 μM at 37 °C for 24, 48, and 72 h. The effects of AF-859 and AG-066 on the cultured HepG2
cells were estimated using an inverted light microscope. Furthermore, the DNA ladder method and the
dead cell assay were performed by applying Live/Dead Cell Staining Kit II. Erk phosphorylation was
estimated by western blotting.

Results:
Treatment with AG-066 markedly reduced HepG2 viable cell counts compared to the AF-
859-treated HepG2 cells, as evident from the significantly increased number of dead cells in the
culture medium. Additionally, AG-066 treatment increased cellular DNA laddering. We found no
difference in Erk phosphorylation status between the AG-066- and AF-859-treated groups.

Conclusion:
This study illustrated that the peptide with a structure based on FAM83G functions as a
spontaneous apoptosis inducer for liver cancer cells. Hence, it is a promising lead compound for the
treatment of liver cancer.
Antiviral Activity of Bee Products
Authors: Kontogiannis Theodoros , Dimitriou Tilemachos G. , Didaras Nikos Asoutis , Mossialos Dimitris 
Published: September 2022

Abstract:
Honey bees provide many products exerting a wide range of benefits to humans. Honey, propolis,
royal jelly, beeswax, bee venom, bee pollen and bee bread have been used as natural medicines since ancient
times because of their therapeutic effects. These products have demonstrated healing properties against wounds,
diabetes, gastrointestinal diseases, cancer, asthma, neurological diseases, bacterial and viral infections. The antibacterial
and antibiofilm activity of honey bee products is widely studied, and a huge body of evidence supports
it. On the other hand, their antiviral effect has not been extensively studied. However, recent research has
demonstrated their potential against various viral infections including SARS-CoV-2. Hence, honey bee products
could be alternatives to treat viral diseases, especially when there is no effective treatment available. This
narrative review aims to present up-to-date data (including ongoing clinical trials) regarding the antiviral activity
of honey bee products, aiming to elucidate how honey bee product supplementation contributes to antiviral
treatment.
The Medicinal Plant Agrimonia pilosa Ledeb.: Botanical Description,
Traditional use, Phytochemistry and Pharmacology
Authors: Hop Nguyen Quang , Son Ninh The 
Published: September 2022

Background:
Hairy agrimony (Agrimonia pilosa Ledeb.) is a traditional medicinal plant
widely used in Eastern Europe and Eastern Asia. The plant is harvested as it comes into flower and
could be dried for later usage. Hairy agrimony has been traditionally introduced to treat sore throat,
abdominal pain, headache, mucoid dysentery, bloody and white discharge, parasites, and eczema.

Objective:
Since the 1950s, various experimental reports relating to phytochemical and pharmacological
aspects have been observed, but an overview is now not available. The current paper emphasizes
on in-depth information about the botanical description, traditional use, phytochemistry, and
pharmacology.

Methods:
The collection of previous research is basically dependent on the reliable resources Sci-
Finder, Google Scholar, ScienceDirect, reputation publishers, and thesis books.

Results:
A. pilosa was found to contain a variety of chemical classes. To date, more than 160 secondary
metabolites have been separated, and the derivatives type flavonoids, phloroglucinols, tannins,
isocoumarins, and triterpenoids are the main components. A. pilosa crude extracts and their
isolates set a broad panel of pharmacological values, including anti-cancer, anti-microbial, antivirus,
anti-oxidant, anti-inflammation, anti-diabetes, anti-osteosarcoma, anti-aging, anti-nociception,
anti-adipogenesis, anti-leishmaniasis, estrogenic-like activity, neuroprotective and hepatoprotective
activities, and vascular relaxation.

Conclusion:
In vitro and in vivo results also successfully explained the pharmacological mechanisms
of A. pilosa constituents. More bioassay-guided phytochemical and clinical studies are necessary.
An Update on Non-invasive Approaches for Genetic Testing of the Preimplantation Embryo
Authors: Kakourou Georgia , Mamas Thalia , Vrettou Christina , Traeger-Synodinos Joanne 
Published: September 2022

Abstract:
Preimplantation Genetic Testing (PGT) aims to reduce the chance of an affected pregnancy
or improve success in an assisted reproduction cycle. Since the first established pregnancies in 1990,
methodological approaches have greatly evolved, combined with significant advances in the embryological
laboratory. The application of preimplantation testing has expanded, while the accuracy and
reliability of monogenic and chromosomal analysis have improved. The procedure traditionally employs
an invasive approach to assess the nucleic acid content of embryos. All biopsy procedures require
high technical skill, and costly equipment, and may impact both the accuracy of genetic testing
and embryo viability. To overcome these limitations, many researchers have focused on the analysis
of cell-free DNA (cfDNA) at the preimplantation stage, sampled either from the blastocoel or embryo
culture media, to determine the genetic status of the embryo non-invasively. Studies have assessed the
origin of cfDNA and its application in non-invasive testing for monogenic disease and chromosomal
aneuploidies. Herein, we discuss the state-of-the-art for modern non-invasive embryonic genetic material
assessment in the context of PGT. The results are difficult to integrate due to numerous methodological
differences between the studies, while further work is required to assess the suitability of
cfDNA analysis for clinical application.
Assessment of Mineralized Nitrogen During Maize Growth Succeeding Different Winter Cover Crops in the Mediterranean Environment
Authors: Perdigão Adelaide , Pereira José L. S. , Moreira Nuno , Trindade Henrique , Coutinho João 
Published: September 2022

Background:
Understanding soil nitrogen (N) dynamics is essential to find alternative N sources and improve N use efficiency in agriculture.

Objective:
The aim of this study was to assess N mineralization rates from residues of winter cover crops, during maize crop season, under Mediterranean conditions.

Methods:
A field experiment was carried out from May to September in central Portugal, with four replications, two sowing dates of cover crops (15/10/2011 and 29/11/2011) and three cover crops residues (balansa clover, ryegrass and yellow lupine) that were incorporated in the soil. Plots were cropped with local maize and net N mineralization was measured during the crop cycle, using soil cylinders placed inside micro-perforated polyethylene bags.

Results and Discussion:
Early sowing of the cover crops residues increased the NH4+ and NO3- contents in the soil. Yellow lupine residue had the highest rate of daily N mineralization (0.71 mg N kg-1 day-1). For all treatments, the highest mineralization rate was found in the last incubation period, ranging between 0.78 mg N kg-1 day-1 and 1.84 mg N kg-1 day-1, both for balansa clover, from the second and the first sowing date, respectively.

Conclusion:
The present study suggests that, under Mediterranean field conditions, cover crops residue of Italian ryegrass, balansa clover and yellow lupin can be used as a nitrogen source namely for sustainable maize crops.