bluuebunny/crossref_metadata_2025_split huggingface dataset:
Direct comparison of different therapeutic cell types susceptibility to inflammatory cytokines associated with COVID-19 acute lung injury
Authors: Vaka Ramana , Khan Saad , Ye Bin , Risha Yousef , Parent Sandrine , Courtman David , Stewart Duncan J. , Davis Darryl R. 
Published: January 2022

Abstract
Background
Although 90% of infections with the novel coronavirus 2 (COVID-19) are mild, many patients progress to acute respiratory distress syndrome (ARDS) which carries a high risk of mortality. Given that this dysregulated immune response plays a key role in the pathology of COVID-19, several clinical trials are underway to evaluate the effect of immunomodulatory cell therapy on disease progression. However, little is known about the effect of ARDS associated pro-inflammatory mediators on transplanted stem cell function and survival, and any deleterious effects could undermine therapeutic efficacy. As such, we assessed the impact of inflammatory cytokines on the viability, and paracrine profile (extracellular vesicles) of bone marrow-derived mesenchymal stromal cells, heart-derived cells, and umbilical cord-derived mesenchymal stromal cells.

Methods
All cell products were manufactured and characterized to established clinical release standards by an accredited clinical cell manufacturing facility. Cytokines and Extracellular vesicles in the cell conditioned media were profiled using proteomic array and nanoparticle tracking analysis. Using a survey of the clinical literature, 6 cytotoxic cytokines implicated in the progression of COVID-19 ARDS. Flow cytometry was employed to determine receptor expression of these 6 cytokines in three cell products. Based on clinical survey and flow cytometry data, a cytokine cocktail that mimics cytokine storm seen in COVID-19 ARDS patients was designed and the impact on cytokine cocktail on viability and paracrine secretory ability of cell products were assessed using cell viability and nanoparticle tracking analysis.

Results
Flow cytometry revealed the presence of receptors for all cytokines but IL-6, which was subsequently excluded from further experimentation. Despite this widespread expression, exposure of each cell type to individual cytokines at doses tenfold greater than observed clinically or in combination at doses associated with severe ARDS did not alter cell viability or extracellular vesicle character/production in any of the 3 cell products.

Conclusions
The paracrine production and viability of the three leading cell products under clinical evaluation for the treatment of severe COVID-19 ARDS are not altered by inflammatory mediators implicated in disease progression.
Automatic discrimination between front and back ensemble locations in HRTF-convolved binaural recordings of music
Authors: Zieliński Sławomir K. , Antoniuk Paweł , Lee Hyunkook , Johnson Dale 
Published: January 2022

AbstractOne of the greatest challenges in the development of binaural machine audition systems is the disambiguation between front and back audio sources, particularly in complex spatial audio scenes. The goal of this work was to develop a method for discriminating between front and back located ensembles in binaural recordings of music. To this end, 22, 496 binaural excerpts, representing either front or back located ensembles, were synthesized by convolving multi-track music recordings with 74 sets of head-related transfer functions (HRTF). The discrimination method was developed based on the traditional approach, involving hand-engineering of features, as well as using a deep learning technique incorporating the convolutional neural network (CNN). According to the results obtained under HRTF-dependent test conditions, CNN showed a very high discrimination accuracy (99.4%), slightly outperforming the traditional method. However, under the HRTF-independent test scenario, CNN performed worse than the traditional algorithm, highlighting the importance of testing the algorithms under HRTF-independent conditions and indicating that the traditional method might be more generalizable than CNN. A minimum of 20 HRTFs are required to achieve a satisfactory generalization performance for the traditional algorithm and 30 HRTFs for CNN. The minimum duration of audio excerpts required by both the traditional and CNN-based methods was assessed as 3 s. Feature importance analysis, based on a gradient attribution mapping technique, revealed that for both the traditional and the deep learning methods, a frequency band between 5 and 6 kHz is particularly important in terms of the discrimination between front and back ensemble locations. Linear-frequency cepstral coefficients, interaural level differences, and audio bandwidth were identified as the key descriptors facilitating the discrimination process using the traditional approach.
LOGO: an efficient local and global data collection mechanism for remote underwater monitoring
Authors: Baalbaki Hussein , Harb Hassan , Rashid Ameer Sardar Kwekha , Jaber Ali , Jaoude Chady Abou , Zaki Chamseddine , Tout Kifah 
Published: January 2022

AbstractThe oceans play an important role in our daily life and they form the lungs of our planet. Subsequently, the world ocean provides so many benefits for humans and the planet including oxygen production, climate regulation, transportation, recreation, food, medicine, economic, etc. However, the oceans suffer nowadays from several challenges ranging from pollution to climate change and destruction of underwater habitat. Hence, the use of remote sensing technologies, like sensor networks and IoT, is becoming essential in order to continuously monitor the wide underwater areas and oceans. Unfortunately, the limited battery power constitutes one of the major challenges and limitations of such technologies. In this paper, we propose an efficient LOcal and GlObal data collection mechanism, called LOGO, that aims to conserve the energy in remote sensing applications. LOGO is based on the cluster scheme and works on two network stages: local and global. The local stage is at the sensor node and aims to reduce its data transmission by eliminating on-period and in-period data redundancies. The global stage is at the autonomous underwater vehicle (AUV) level and aims to minimize the data redundancy among neighboring nodes based on a spatial-temporal node correlation and Kempe’s graph techniques. The simulation results on real underwater data confirm that LOGO mechanism is less energy consumption with high data accuracy than the existing techniques.
Prevalence and characterization of antimicrobial resistance among gram-negative bacteria isolated from febrile hospitalized patients in central Ethiopia
Authors: Tufa Tafese Beyene , Mackenzie Colin R. , Orth Hans Martin , Wienemann Tobias , Nordmann Tamara , Abdissa Sileshi , Hurissa Zewdu , Schönfeld Andreas , Bosselmann Matthias , Häussinger Dieter , Pfeffer Klaus , Luedde Tom , Fuchs Andre , Feldt Torsten 
Published: January 2022

Abstract
Background
Infectious diseases are among the leading causes of death in many low-income countries, such as Ethiopia. Without reliable local data concerning causative pathogens and antimicrobial resistance, empiric treatment is suboptimal. The objective of this study was to characterize gram-negative bacteria (GNB) as pathogens and their resistance pattern in hospitalized patients with infections in central Ethiopia.

Methods
Patients ≥ 1 year of age with fever admitted to the Asella Referral and Teaching Hospital from April 2016 to June 2018 were included. Blood and other appropriate clinical specimens were collected and cultured on appropriate media. Antibiotic susceptibility testing (AST) was performed using the Kirby–Bauer method and VITEK® 2. Species identification and detection of resistance genes were conducted using MALDI-ToF MS (VITEK® MS) and PCR, respectively.

Results
Among the 684 study participants, 54.2% were male, and the median age was 22.0 (IQR: 14–35) years. Blood cultures were positive in 5.4% (n = 37) of cases. Among other clinical samples, 60.6% (20/33), 20.8% (5/24), and 37.5% (3/8) of swabs/pus, urine and other body fluid cultures, respectively, were positive. Among 66 pathogenic isolates, 57.6% (n = 38) were GNB, 39.4% (n = 26) were gram-positive, and 3.0% (n = 2) were Candida species. Among the isolated GNB, 42.1% (16/38) were Escherichia coli, 23.7% (9/38) Klebsiella pneumoniae and 10.5% (4/38) Pseudomonas aeruginosa.
In total, 27/38 gram-negative isolates were available for further analysis. Resistance rates were as follows: ampicillin/sulbactam, 92.6% (n = 25); cefotaxime, 88.9% (n = 24); ceftazidime, 74.1% (n = 20); cefepime, 74.1% (n = 20); gentamicin, 55.6% (n = 15); piperacillin/tazobactam, 48.1% (n = 13); meropenem, 7.4% (n = 2); and amikacin, 3.7% (n = 1). The blaNDM-1 gene was detected in one K. pneumoniae and one Acinetobacter baumannii isolate, which carried an additional blaOXA-51 gene. The ESBL enzymes were detected in 81.5% (n = 22) of isolates as follows: TEM, 77.2% (n = 17); CTX-M-1 group, 68.2% (n = 15); SHV group, 27.3% (n = 6); and CTX-M-9 group, 9.1% (n = 2). Based on the in vitro antimicrobial susceptibility results, empiric treatment initiated in 13 of 18 (72.2%) patients was likely ineffective.

Conclusion
We report a high prevalence of ESBL-producing bacteria (81.5%) and carbapenem resistance (7.4%), with more than half of GNB carrying two or more ESBL enzymes resulting in suboptimal empiric antibiotic therapy. These findings indicate a need for local and national antimicrobial resistance surveillance and the strengthening of antimicrobial stewardship programs.
GPS-based fine-scale mapping surveys for schistosomiasis assessment: a practical introduction and documentation of field implementation
Authors: Trippler Lydia , Ali Mohammed Nassor , Ame Shaali Makame , Ali Said Mohammed , Kabole Fatma , Hattendorf Jan , Knopp Stefanie 
Published: January 2022

Abstract
Background
Fine-scale mapping of schistosomiasis to guide micro-targeting of interventions will gain importance in elimination settings, where the heterogeneity of transmission is often pronounced. Novel mobile applications offer new opportunities for disease mapping. We provide a practical introduction and documentation of the strengths and shortcomings of GPS-based household identification and participant recruitment using tablet-based applications for fine-scale schistosomiasis mapping at sub-district level in a remote area in Pemba, Tanzania.

Methods
A community-based household survey for urogenital schistosomiasis assessment was conducted from November 2020 until February 2021 in 20 small administrative areas in Pemba. For the survey, 1400 housing structures were prospectively and randomly selected from shapefile data. To identify pre-selected structures and collect survey-related data, field enumerators searched for the houses’ geolocation using the mobile applications Open Data Kit (ODK) and MAPS.ME. The number of inhabited and uninhabited structures, the median distance between the pre-selected and recorded locations, and the dropout rates due to non-participation or non-submission of urine samples of sufficient volume for schistosomiasis testing was assessed.

Results
Among the 1400 randomly selected housing structures, 1396 (99.7%) were identified by the enumerators. The median distance between the pre-selected and recorded structures was 5.4 m. A total of 1098 (78.7%) were residential houses. Among them, 99 (9.0%) were dropped due to continuous absence of residents and 40 (3.6%) households refused to participate. In 797 (83.1%) among the 959 participating households, all eligible household members or all but one provided a urine sample of sufficient volume.

Conclusions
The fine-scale mapping approach using a combination of ODK and an offline navigation application installed on tablet computers allows a very precise identification of housing structures. Dropouts due to non-residential housing structures, absence, non-participation and lack of urine need to be considered in survey designs. Our findings can guide the planning and implementation of future household-based mapping or longitudinal surveys and thus support micro-targeting and follow-up of interventions for schistosomiasis control and elimination in remote areas.
Trial registration ISRCTN, ISCRCTN91431493. Registered 11 February 2020, https://www.isrctn.com/ISRCTN91431493
Impact of eating disorders on paid or unpaid work participation and performance: a systematic review and meta-analysis protocol
Authors: Safi Fatima , Aniserowicz Anna M. , Colquhoun Heather , Stier Jill , Nowrouzi-Kia Behdin 
Published: January 2022

Abstract
Background
Eating disorders (ED) can reduce quality of life by limiting participation and performance in social and occupational roles, including paid or unpaid work. The association between ED pathologies and work participation and performance must be well understood to strengthen vocational rehabilitation programmes and prevent occupational disruptions in the ED population. The aims of this study are: (1) to examine the degree of association between ED pathologies and work participation and performance in 15-year-olds and older; (2) to highlight the specific ED symptoms that are most correlated with changes in work performance and participation; (3) to compile the most common metrics and assessments used to measure work participation and performance with ED.

Methods
Medline, Embase, CINAHL, Web of Science, PsycINFO, and Cochrane Library will be searched for observational and experimental studies that meet the following criteria: (1) a clinical sample of typical or atypical ED; (2) paid or unpaid employment or training; (3) an association between ED pathologies and work participation or performance. Unpublished data will also be examined. Title and abstract, and full-text screening will be conducted in duplicate. Risk of bias and quality of evidence assessments will be completed. A random-effect meta-analysis will be performed.

Discussion
This synthesis can clarify knowledge and gaps around the impact of ED on work functioning, thereby allowing better evaluation, improvements and development of current workplace assessments, interventions, and policies.

Trial registration

The registration number for this systematic review on PROSPERO is CRD42021255055.
Attachment and grief in young adults after the loss of a close friend: a qualitative study
Authors: Johnsen Iren , Tømmeraas Ane Martine 
Published: January 2022

Abstract
Background
Although many lose a close friend each year, they are seldom the focus of grief research. However, these losses often cause severe and long-lasting reactions. Deaths among adolescents and young adults are also often caused by traumatic events, e.g. from accidents, suicides, and homicides, placing them at significant risk for complicated grief reactions. The focus of this paper is bereaved friends after the shootings at Utøya, Norway in 2011, which is among a few studies that focus on bereaved friends, exploring how attachment affects the grief process after the loss of a close friend.

Methods
This paper explores qualitative data from in-depth interviews with thirteen bereaved friends, conducted about 28 months after the loss. The interview sample consisted of eight females and five males, aged 18–31 years. The interviews were semi-structured, with a theme guide of 14 questions, and the method used for analyses was systematic text condensation.

Results
Two main themes were identified from the analyses of the interviews: Friendship and Grief, with the subordinate themes: The importance of the friendship, Longing and remembrance, How the loss has affected other relationships, How the loss has affected the friend’s daily lives, Processing of the grief and Not being family. For most of the bereaved friends the loss and the grief had a profound effect on them and their overall lives, from daily functioning in school or at work, to changes in attitudes, and the way they were met as bereaved.

Conclusions
The support, intimacy, and feelings of togetherness we share with our friends are of great importance and value for all people, but maybe especially for young people. When adolescents and young adults experience losses, their reactions are often intense and long-lasting, and especially complicated grief reactions can affect school performance and concentration, health, result in emotional problems; and disrupt development (e.g. identity formation and social skills). We don’t know much about the grief of bereaved friends and how their reactions can be explained. Thus, we hope that these findings could shed light on their grief reactions, and provide new knowledge on the short- and long-term psychological impact of losses of friends.
Sex-dependent effects of long-term clozapine or haloperidol medication on red blood cells and liver iron metabolism in Sprague Dawley rats as a model of metabolic syndrome
Authors: Bouvier Marie-Luise , Fehsel Karin , Schmitt Andrea , Meisenzahl-Lechner Eva , Gaebel Wolfgang , von Wilmsdorff Martina 
Published: January 2022

Abstract
Background
Patients with liver diseases often have some form of anemia. Hematological dyscrasias are known side effects of antipsychotic drug medication and the occurrence of agranulocytosis under clozapine is well described. However, the sex-dependent impact of clozapine and haloperidol on erythrocytes and symptoms like anemia, and its association with hepatic iron metabolism has not yet been completely clarified. Therefore, in the present study, we investigated the effect of both antipsychotic drugs on blood parameters and iron metabolism in the liver of male and female Sprague Dawley rats.

Methods
After puberty, rats were treated orally with haloperidol or clozapine for 12 weeks. Blood count parameters, serum ferritin, and liver transferrin bound iron were determined by automated counter. Hemosiderin (Fe3+) was detected in liver sections by Perl’s Prussian blue staining. Liver hemoxygenase (HO-1), 5’aminolevulinate synthase (ALAS1), hepcidin, heme-regulated inhibitor (HRI), cytochrome P4501A1 (CYP1A1) and 1A2 (CYP1A2) were determined by Western blotting.

Results
We found anemia with decreased erythrocyte counts, associated with lower hemoglobin and hematocrit, in females with haloperidol treatment. Males with clozapine medication showed reduced hemoglobin and increased red cell distribution width (RDW) without changes in erythrocyte numbers. High levels of hepatic hemosiderin were found in the female clozapine and haloperidol medicated groups. Liver HRI was significantly elevated in male clozapine medicated rats. CYP1A2 was significantly reduced in clozapine medicated females.

Conclusions
The characteristics of anemia under haloperidol and clozapine medication depend on the administered antipsychotic drug and on sex. We suggest that anemia in rats under antipsychotic drug medication is a sign of an underlying liver injury induced by the drugs. Changing hepatic iron metabolism under clozapine and haloperidol may help to reduce these effects of liver diseases.
Identification and mapping of ancient pigments in a Roman Egyptian funerary portrait by application of reflectance and luminescence imaging spectroscopy
Authors: Radpour Roxanne , Gates Glenn A. , Kakoulli Ioanna , Delaney John K. 
Published: January 2022

Abstract
Imaging spectroscopy (IS) is an important tool in the comprehensive technical analysis required of archaeological paintings. The complexity of pigment mixtures, diverse artistic practices and painting technologies, and the often-fragile and weathered nature of these objects render macroscale, non-invasive chemical mapping an essential component of the analytical protocol. Furthermore, the use of pigments such as Egyptian blue and madder lake, featuring diagnostic photoluminescence emission, provides motivation to perform photoluminescence mapping on the macroscale. This work demonstrates and advances new applications of dual-mode imaging spectroscopy and data analysis approaches for ancient painting. Both reflectance (RIS) and luminescence (LIS) modes were utilized for the study of a Roman Egyptian funerary portrait from second century CE Egypt. The first derivative of the RIS image cube was analyzed and found to significantly improve materials separation, identification, and the extent of mapping. Egyptian blue and madder lake were mapped across a decorated surface using their luminescence spectral signatures in the region of 540–1000 nm as endmembers in LIS analyses. Linear unmixing of the LIS endmembers and subsequent derivative analyses resulted in an improved separation and mapping of the luminescence pigments. RIS and LIS studies, combined with complementary, single-spot collection elemental and molecular spectroscopy, were able to successfully characterize the portrait’s painting materials and binding media used by the ancient artist, providing key insight into their material use, stylistic practices, and technological choices.
Extraction of underwater fragile artifacts: research status and prospect
Authors: Chen Xue-Qiang , Xia Ke , Hu Wenjing , Cao Ming , Deng Kai , Fang Shiqiang 
Published: January 2022

Abstract
There are still many difficulties in the recovery and long-term preservation of underwater archaeological artifacts, in situ preservation should be the first choice before further procedures are considered. However, the materials, preservation status, and preservation environment of underwater artifacts are diverse, resulting in many fragile artifacts facing difficult situations. In order to prevent serious damage, it is a safe protective strategy to preserve them in a controlled environment for a long time after excavation. Extraction and transfer of fragile cultural relics are vital parts of this strategy. Due to the complexity of the underwater environment and the vulnerability of fragile artifacts, safety in extraction and transfer still faces enormous challenges. Researchers have developed new materials and technologies to tackle this problem. This paper focuses on introducing and developing prospects to different preservation techniques for fragile artifacts from underwater sites.