bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hydrogeochemical vertical zonation and evolution model of the Kongjiagou coalmine in Sichuan, China
Authors: Heng Yin , Yong Wu , Lili Jiang , Meng Chen , Nisong Pei , Yong Luo , Li Lyu 
Published: May 2022

Abstract
With mine drainage and activities, the hydrogeochemical environments has greatly changed in Kongjiagou coalmine, Sichuan Province, southwest China. This study aimed to summarize an ideal model of coalmine hydrogeochemical zonation and evolution. In total, 24 groundwater samples from different depth were collected to analyze their components, with the combination of methods such as ion sources, normalization index, ions ratios, cation exchange and inverse modeling. The hydrogeochemical formation main factor was water–rock reactions and more weathering by sulfuric acid. Dissolution and pyrite oxidation were the main sources of , whereas carbonate weathering was the dominant source of . The vertical extent could be distinguished to three zones by mean and standard deviation of burial depth. The normalization index, ions ratios, cation exchange and water types exhibited clear vertical zonation. In shallow groundwater, dissolution led to a Ca- type water and overall stable concentrations of ions. Redox reactions in the middle region have caused significant increases in the total dissolved solids (TDS) and and a considerable reduction in pH; hence, Ca + Mg-SO4 + HCO3 became the dominant type. Re-dissolution and cation exchange played important roles at depth and caused the pH and TDS to increase slowly. The , Ca2+, and Mg2+ concentrations increased rapidly, whereas the concentration remained basically unchanged, and the water type was either Ca + Mg-HCO3 + SO4 or Na-HCO3 + SO4. This study will be of great significance to coalmine safety production and water environments protection.
Review on some metal oxide nanoparticles as effective adsorbent in wastewater treatment
Authors: Ghosh Nikita , Das Susmita , Biswas Goutam , Haldar Prabir Kumar 
Published: May 2022

Abstract
Water contamination has turned into one of the most serious issues in the world. Nanomaterials are proficient to carry away heavy metals, organic and inorganic dyes, pesticides, and small molecules from polluted water. In this regard, nanoparticles have gained much attention due to their extraordinary properties compared to bulk materials. Metal oxide nanoparticles and nanocomposites have several advantages such as elevated surface area, low concentration, easily separable after treatment and so on. Among many feasible techniques, the adsorption process is one of the most useful techniques for removing heavy ions and dyes from wastewater and has gained much attention from researchers. Several studies on metal oxide nanoparticles and their use in wastewater treatment have been published in the literature. This chapter gives an outline about five metal oxide based nanomaterials and nanocomposites as well as their applications in water pollution removal where the efficiency, limits and favourable circumstances are compared and explored. This article surely helps to gather information about some metal oxide nanoparticles and nanocomposites in wastewater treatment by the adsorption technique. In this review article, we primarily focused on five metal oxide nanoparticles and some of their recent applications published in the last two years.
Waste eggshell-supported CuO used as heterogeneous catalyst for reactive blue 19 degradation through peroxymonosulfate activation (CuO/eggshell catalysts activate PMS to degrade reactive blue 19)
Authors: Xu Jinghua , Hu Sisi , Min Lingli , Wang Shuhua 
Published: May 2022

Abstract
Advanced oxidation processes play an important role in the removal of organic pollutants from wastewater, in which it is essential to develop an eco-friendly, effective, stable, and inexpensive catalyst. Herein, waste eggshell-supported copper oxide (CuO/eggshell) was synthesized via a facile method and employed as peroxymonosulfate (PMS) activator for the elimination of reactive blue 19 (RB19). CuO/eggshell exhibited high degradation efficiency of RB19 (approximately 100%) by activation of PMS under the optimum conditions of 20 mg/L RB19, 0.2 g/L CuO/eggshell, 0.36 mM PMS, and initial pH 7.12 within 20 min. In addition, the effects of catalyst dosage, PMS concentration, initial pH, inorganic ions, and humic acid on RB19 degradation were investigated. Scavenging experiments and electron paramagnetic resonance revealed that multiple reactive oxygen species, including sulfate radicals (SO4·−), hydroxyl radicals (·OH), superoxide radicals (O2·−), and singlet oxygen (1O2), contributed to RB19 degradation, and 1O2 played a dominant role. Finally, a possible PMS activation mechanism was proposed. This study suggests that loading catalytically active components onto waste eggshell is eco-friendly and effective for enhancing the degradation of dyes from wastewater.
Growth and photosynthesis responses of microcystin (MC)- and non-MC-producing Microcystis strains during co-culture with the submerged macrophyte Myriophyllum spicatum
Authors: Gao Yunni , Yang Hui , Dong Jing , Zhang Man , Gao Xiaofei , Zhang Jingxiao , Li Mei , Li Xuejun 
Published: May 2022

Abstract
The growth and photosynthetic responses of microcystin (MC)- and non-MC-producing Microcystis to the submerged macrophyte Myriophyllum spicatum were investigated under plant-Microcystis co-culture conditions (PMC + , PMC−), in comparison with their corresponding mono-culture controls (SMC + , SMC-). The OJIP chlorophyll a fluorescence transient was recorded and analyzed using JIP-test parameters. In comparison with the corresponding mono-culture controls, the quantum efficiencies of the electron transport chain expressed as parameters Ψo and φEo decreased by more than 25% in the PMC− group, much higher than those in the PMC+ group (p &lt; 0.05). The decreasing extent of the electron transport flux ratio ETo/RC was significantly larger in the PMC− group than in the PMC+ group (p &lt; 0.05). The performance index (PIABS) decreased by 26.98% in the PMC− group and by 16.25% in the PMC+ group. These results indicated that the non-MC-producing Microcystis was more sensitive than the MC-producing strain when co-cultured with M. spicatum, and the efficiency and energy flux of electron transport might be the main targets of Photosystem II. Future research should focus on the intraspecific composition and toxigenic levels of Microcystis populations interacting with submerged macrophytes to guide the use of submerged vegetation restoration.
The Role of Heat Shock Protein 40 in Carcinogenesis and Biology of Colorectal
Cancer
Authors: Asgharzadeh Fereshteh , Moradi-Marjaneh Reyhaneh , Marjaneh Mahdi Moradi 
Published: May 2022

Abstract:
Colorectal cancer (CRC) is the third most common cancer worldwide. Despite the enormous amount
of effort in the diagnosis and treatment of CRC, the overall survival rate of patients remains low. The precise
molecular and cellular basis underlying CRC has not been completely understood yet. Over time, new genes
and molecular pathways involved in the pathogenesis of the disease are being identified. The accurate discovery
of these genes and signaling pathways are important and urgent missions for the next generation of anticancer
therapy research. Chaperone DnaJ, also known as Hsp40 (heat shock protein 40), has been of particular interest
in CRC pathogenesis, as it is involved in the fundamental cell activities for maintaining cellular homeostasis.
Evidence shows that protein family members of DnaJ/Hsp40 play both roles, enhancing and reducing the
growth of CRC cells. In the present review, we focus on the current knowledge of the molecular mechanisms
responsible for DnaJ/Hsp40 in CRC carcinogenesis and biology.
Syringic Acid Suppressed Proliferation, Invasion, and Migration via
Inhibition of Matrix Metalloproteinases Expression on Glioblastoma Cells
by Promoting Apoptosis
Authors: Peng Biao , Li Dan , Luo Dongdong , Hu Su , Zhao Hailin 
Published: May 2022

Background:
Human brain tumor glioblastoma (GBM) is the most hostile malignancy,
currently lacking a successful cure and good prognosis.

Objective:
To examine the anticancer effects of syringic acid (SA) on human cancer GBM cells.

Methodology:
The different doses of SA were added to GBM cells to study its effect on viability,
invasion, relocation, apoptosis, and mRNA and protein levels. Hence, we explored the antiproliferative,
anti-invasive, and apoptotic activity of SA on GBM human U-251 cells.

Results:
MTT assay and live/dead assay revealed the anti-proliferative activity of SA on U-251
glioma cells. Apoptotic activity of SA was shown by DAPI staining, caspase-3, Bax, and Bcl-2
mRNA expressions. The cell cycle regulation was also confirmed by reducing the mRNA expression
of cyclinD1, CDK4, and CDK6. Treatment of SA with U-251 cells suppressed MMPs expressions
and enhanced TIMPs protein levels.

Conclusion:
Our findings put forward that SA could prevent GBM cells’ invasion and relocation.
SA is an ideal neuroprotective agent for controlling brain malignancy.
Recent Studies on the Pharmacological Activities and Structural Modifications
of Compound-K
Authors: Zhang Mingming , Li Yingxia , Wang Xianyang 
Published: May 2022

:
Ginsenosides, the essential active ingredients extracted from ginseng, have been well
studied in the past several decades because of their numerous pharmacological properties including
anti-tumor, anti-inflammatory, and anti-diabetic activities, as well as hepatoprotection, skin
protection, and memory improvement, etc. Compound-K (CK) is the major metabolite derived
from the deglycosylation of ginsenosides by intestinal bacteria and has been proved to be the actual
active entity absorbed into the systemic circulation. In this review, we comprehensively elucidate
the pharmacological activities of CK from the molecular mechanism, as well as its structurally
modified derivatives. We hope this review would be helpful to get a systematic summary and
provide constructive insights for the further research of CK.
Verapamil Regulates the Macrophage Immunity to Mycobacterium
tuberculosis through NF-κB Signaling
Authors: Gong Wenping , Cui Ruina , Song Lele , Yang Yourong , Zhang Junxian , Liang Yan , Bai Xuejuan , Wang Jie , Wang Lan , Wu Xueqiong , Zhao Weiguo 
Published: May 2022

Background:
Verapamil enhances the sensitivity of Mycobacterium
tuberculosis to anti-tuberculosis (TB) drugs, promotes the macrophage anti-TB ability,
and reduces drug resistance, but its mechanism is unclear. Herein, we have investigated
the effect of verapamil on cytokine expression in mouse peritoneal macrophages.

Methods:
Macrophages from mice infected with M. tuberculosis or S. aureus were
cultured with verapamil, the cytokines were detected by enzyme-linked immunosorbent
assay, and the RNA was measured with quantitative real-time polymerase chain
reaction and agarose gel electrophoresis. The intracellular calcium signaling was
measured by confocal microscopy.

Results:
Significantly higher levels of NF-κB, IL-12, TNF-α, and IL-1β were observed
after TB infection. The levels of NF-κB and IL-12 increased when verapamil
concentration was less than 50 μg/ml, but decreased when verapamil concentration was
greater than 50μg/ml. With the increase in verapamil concentration, TNF-α and IL-1β
expressed by macrophages decreased. The L-type calcium channel transcription
significantly increased in M. tuberculosis rather than S. aureus-infected macrophages.
Furthermore, during bacillus Calmette-Guerin (BCG) infection, verapamil stimulated a
sharp peak in calcium concentration in macrophages, while calcium concentration
increased mildly and decreased smoothly over time in the absence of verapamil.

Conclusions:
Verapamil enhanced macrophage immunity via the NF-κB pathway, and its
effects on cytokine expression may be achieved by its regulation of intracellular calcium
signaling.
Natural Flavonoid Quercetin Enhances the Anti-inflammatory
Effects of Aspirin in a Preeclampsia-like Rat Model Induced by
Lipopolysaccharide
Authors: Chen Dan , Yang Shuangyan , Ding Jie , Liu Ailing 
Published: May 2022

Background:
Preeclampsia is a disorder of hypertension and proteinuria
accompanied by abnormal inflammatory responses. Both aspirin and quercetin possess
anti-inflammatory and anti-hypertensive properties. A low dose of aspirin is
recommended for the prevention of preeclampsia in patients with preeclampsia history.
Whether quercetin can enhance the effect of aspirin on preeclampsia remains elusive.

Methods:
Female Sprague-Dawley pregnant rats were treated with daily administration
of aspirin, quercetin, or a combination of aspirin and quercetin and subsequently
received lipopolysaccharides (LPS) injection to induce preeclampsia-like symptoms. The
systolic blood pressure and proteinuria from all groups of rats were assessed.

Results:
Our results demonstrated that the combination of quercetin and aspirin exerted
significantly stronger effects than aspirin alone on decreasing systolic blood pressure
and proteinuria, reducing pro-inflammatory cytokine production, and inhibiting M1-type
decidual macrophages polarization in an LPS-induced rat model of preeclampsia.

Conclusion:
This study suggested that quercetin may serve as an excellent supplement
to aspirin in preventing or treating patients with preeclampsia.
A Review of the Use of Metallic Nanoparticles as a Novel Approach for
Overcoming the Stability Challenges of Blood Products: A Narrative
Review from 2011-2021
Authors: Mehrizi Tahereh Zadeh , Ardestani Mehdi Shafiee , Kafiabad Sedigheh Amini 
Published: May 2022

Purpose:
To obtain safe and qualified blood products (e.g., platelets, plasma, and red blood
cells), various limitations such as limited shelf life (especially for platelets) and stability must be addressed.
In this review study, the most commonly used metal nanomaterials (e.g., gold, silver, iron, and
magnetic) reported in the literature from 2011 to 2021 were discussed owing to their unique properties,
which provide exciting approaches to overcome these limitations and improve the stability, safety, and
quality of blood products.

Novelty:
This study reviews for the first time the results of studies (from 2011 to 2021) that consider the
effects of various metallic nanoparticles on the different blood products.

Results:
The results of this review study showed that some metallic nanoparticles are effective in improving
the stability of plasma proteins. For this purpose, modified Fe3O4 magnetic nanoparticles and
citrate-AuNPs protect albumin products against stressful situations. Also, SiO2 microspheres and silicacoated
magnetite nanoparticles are highly capable of improving IgG stability. ZnO nanoparticles also
reduced thrombin production, and protein-coated GMNP nanoparticles prevented unwanted leakage of
factor VIII through blood vessels. Furthermore, the stability and longevity of erythrocytes can be improved
by AuNP nanoparticles and Zr-based organic nanoparticles. In addition, platelet storage time can
be improved using PEGylated Au and functionalized iron oxide nanoparticles.

Suggestion:
According to the results of this study, it is suggested that further research should be conducted
on metal nanoparticles as the most promising candidates to prepare metal nanoparticles with
improved properties to increase the stability of various blood products.
Netupitant-palonosetron (NEPA) for Preventing Chemotherapy-induced
Nausea and Vomiting: From Clinical Trials to Daily Practice
Authors: Aapro Matti , Jordan Karin , Scotté Florian , Celio Luigi , Karthaus Meinolf , Roeland Eric 
Published: May 2022

abstract:
Chemotherapy-induced nausea and vomiting (CINV) is a common adverse event associated
with many anticancer therapies and can negatively impact patients' quality of life and potentially
limit the effectiveness of chemotherapy. Currently, CINV can be prevented in most patients with
guideline-recommended antiemetic regimens. However, clinicians do not always follow guidelines,
and patients often face difficulties adhering to their prescribed treatments. Therefore, approaches to
increase guideline adherence need to be implemented. NEPA is the first and only fixed combination
antiemetic, composed of netupitant (oral)/fosnetupitant (intravenous) and palonosetron, which, together
with dexamethasone, constitute a triple antiemetic combination recommended for the prevention
of CINV for patients receiving highly emetogenic chemotherapy and for certain patients receiving
moderately emetogenic chemotherapy. Thus, NEPA offers a convenient and straightforward antiemetic
treatment that could improve adherence to guidelines. This review provides an overview of
CINV, evaluates the accumulated evidence of NEPA's antiemetic activity and safety from clinical trials
and real-world practice, and examines the preliminary evidence of antiemetic control with NEPA
in daily clinical settings beyond those described in pivotal trials. Moreover, we review the utility of
NEPA in controlling nausea and preserving patients’ quality of life during chemotherapy, two major
concerns in managing patients with cancer.
COVID-19 and Kidney Disease: A Clinical Perspective
Authors: Theofilis Panagiotis , Vordoni Aikaterini , Kalaitzidis Rigas G. 
Published: May 2022

:
Coronavirus disease-19 (COVID-19), caused by severe acute respiratory syndrome Coronavirus-
2 (SARS-CoV-2), has caused a global pandemic with high morbidity and mortality. The presence
of several comorbidities has been associated with a worse prognosis, with chronic kidney disease being
a critical risk factor. Regarding COVID-19 complications, other than classical pneumonia and thromboembolism,
acute kidney injury (AKI) is highly prevalent and represents a poor prognostic indicator
linked to increased disease severity and mortality. Its pathophysiology is multifactorial, revolving
around inflammation, endothelial dysfunction, and activation of coagulation, while the direct viral insult
of the kidney remains a matter of controversy. Indirectly, COVID-19 AKI may stem from sepsis, volume
depletion, and administration of nephrotoxic agents, among others. Several markers have been
proposed for the early detection of COVID-19 AKI, including blood and urinary inflammatory and kidney
injury biomarkers, while urinary SARS-CoV-2 load may also be an early prognostic sign. Concerning
renal replacement therapy (RRT), general principles apply to COVID-19 AKI, but sudden RRT
surges may mandate adjustments in resources. Following an episode of COVID-19 AKI, there is a gradual
recovery of kidney function, with pre-existing renal impairment and high serum creatinine at discharge
being associated with kidney disease progression and long-term dialysis dependence. Finally,
kidney transplant recipients represent a special patient category with increased susceptibility to COVID-
19 and subsequent high risk of severe disease progression. Rates of mortality, AKI, and graft rejection
are significantly elevated in the presence of COVID-19, highlighting the need for prevention and careful
management of the disease in this subgroup.
Dolutegravir Plus Lamivudine as Initial Therapy for HIV-1 Infected and
ARV-naïve Patients in West China, 24-Weeks Results of a Preliminary
Real-world Study
Authors: Sun Wei , Hui Xia , Gan Xinrong , Li Qian 
Published: May 2022

Introduction:
This preliminary real-world study (RWS) was designed to evaluate the antiviral
efficacy, safety, and feasibility of the 2-drug regimen (2DR), dolutegravir plus lamivudine as the initial
antiretroviral therapy (ART) among antiretroviral (ARV)-naïve adults with HIV-1 in West China.

Methods:
This RWS included the treatment of treatment-naïve adults applying 2DR of dolutegravir
50 mg once daily (QD) plus lamivudine 300mg QD with negative HBsAg from one single center of
People’s Hospital of Chongqing Banan District in West China. Viral load (VL), CD4+ T-cell count,
and laboratory indicators were collected at baseline; weeks 4, 12, and 24, and thereafter every 24
weeks up to 144 weeks. The primary endpoint was the proportion of patients with HIV-1 RNA ＜50
copies/mL at week 24.

Results:
A total of 54 ART-naïve patients were treated with the 2-drug regimen of DTG plus 3TC
and were enrolled in this study since April 1st, 2020. Twenty-one patients received 24-week VL
tests at screening as required by inclusion criteria. Median HIV-1 RNA at entry was 95,700 copies/
mL (interquartile range (IQR): 28,300-310,000) and the median baseline CD4+ cell count was
249 per cubic millimetre(IQR: 118-310). At week 24, 15 (71.4%) of 21 participants achieved virological
success, defined as HIV-1 RNA ＜ 50 copies/mL, while 10 (90.9%) of 11 participants with a
baseline HIV-1 RNA ＜ 100,000 copies/mL achieved virological success compared with 5 (50%) of
10 participants with a baseline HIV-1 RNA ≥100,000 copies/mL [Relative Risk (RR) 1.818; 95%
CI 1.018-1.927]. In participants with CD4+ cell counts ≥ 200 cells/mm3, 9 (75%) of 12 participants
achieved virological success compared with 6 (66.7%) of 9 participants with baseline CD4+ cell
count ＜ 200 cells/mm3 achieved it (RR 1.124; 95% CI 0.641-1.970). No major tolerability/toxicity
issues were observed.

Conclusions:
This real-world study suggested that the 2-drug regimen of DTG plus 3TC could be
considered as an alternative for ART-naïve patients in West China, especially with HIV-1 RNA less
than 100,000 copies/mL at baseline, regarding the limits of viral load test frequency and the absence
of HIV genotypic testing of viral resistance.
Methanol and Sorbitol Affect the Molecular Dynamics of Arginine Deiminase:
Insights for Improving its Stability
Authors: Negahdaripour Manica , Zarei Mahboubeh , Sabetian Soudabeh , Rahbar Mohammad Reza 
Published: May 2022

Background:
Arginine deiminase enzyme of Mycoplasma arginini (MaADI) is a potential anti-cancer agent for treating arginine-auxotrophic cancers. Investigating the protein stability in the presence of osmolytes can help to increase protein stability under various stressed conditions.

Methods:
In this study, the stability and dynamics of MaADI were investigated in pure water and solutions of 1 M sorbitol, 10% (v/v) methanol, and 50% (v/v) methanol using molecular dynamics simulation.

Results:
Sorbitol was found to stabilize the protein, whereas high-concentrated methanol destabilized it. Sorbitol molecules interacted with the protein through hydrogen bonding and reduced the protein fluctuations as well. At 50% methanol, the flexibility of regions 4-8, 195-201, 314-324, and 332-337 in the MaADI was increased; whereas residues 195-201 showed the highest variations.

Conclusion:
Thus, these regions of MaADI, especially 195-201, are the most sensitive regions in the presence of denaturing agents and can be subjected to protein engineering toward improving the stability of MaADI.