bluuebunny/crossref_metadata_2025_split huggingface dataset:
From Waste to Waste: Iron Blast Furnace Slag for Heavy Metal Ions Removal from Aqueous System
Authors: Abdelbasir Sabah M. , Khalek Mohamed A. Abdel 
Published: January 2022

Abstract
Blast furnace slag (BFS) is considered a cheap sorbent for the get rid of Co2+ and Pb2+ ions from an aqueous medium. The slag is characterized using X-ray diffraction (XRD), X-ray fluorescence (XRF), N2 adsorption-desorption isotherms, energy dispersive X-ray analysis (EDX), scanning electron microscopy (SEM), and zeta potential. The removal of Co2+ and Pb2+ ions was carried out using batch adsorption experiments from an aqueous medium. The influence of several variables as pH, duration, sorbent quantity, temperature, and preliminary ions concentration was considered. The isotherm, kinetic, thermodynamic, and recyclability were also conducted. The maximum uptake capacity for Co2+ and Pb2+ was 43.8 and 30.2 mg g-1 achieved at pH 6 after 60 min. contact duration. The adsorption kinetics and isotherms of BFS for Co2+ and Pb2+ fitted well to Avrami and Freundlich models, respectively. The main sorption mechanism between BFS and the metal ions was ion exchange. The regeneration of the used slag was studied for reuse many cycles. In terms of economics and scalability, the treatment with the unmodified BFS has great potentials.
The Association Between Dietary Insulin Index and Load With Mental Health
Authors: Amirinejad Ali , Salehi-Abargouei Amin , Darand Mina , Davies Ian G. , Mirzaei Masoud , Mazidi Mohsen , Khayyatzadeh Sayyed Saeid 
Published: January 2022

Abstract
Background: Depression, anxiety, and stress are common mental problems in different populations. The aim of this cross-sectional study is to investigate the association between dietary insulin index (DII) and insulin load (DIL) and psychological disorders. Method: Participants (n = 10,000) aged 20-69 were randomly selected from 200 clusters in Yazd from the recruitment phase of Yazd Health Study. Dietary intake of study participants was collected by a reliable and validated food frequency questionnaire (FFQ) consisting of 178 food items. DII and DIL were calculated from the FFQ data using previously published reference values. To assess psychological disorders an Iranian validated short version of a self-reported questionnaire (Depression Anxiety Stress Scales 21 [DASS21]) was used.Results: No significant association was observed between DIL and DII with odds of depression or anxiety in crude and adjusted models. The individuals in the highest quartiles of DIL had the lowest odds of stress (OR: 0.69; 95% CI: 0.48–1.01, P-trend = 0.047). This association remained significant after adjustment for potential confounders in model II (OR: 0.38; 95% CI: 0.16–0.91, P-trend = 0.039) and the third and final model (OR: 0.39; 95% CI: 0.16–0.91, P-trend = 0.041).Conclusion: Overall, consumption of foods with higher II as well as IL was associated with lower feelings of stress; however, no significant relation was observed between DII or DIL and risk of depression and anxiety. Further studies with longitudinal design are needed to confirm these results.
Topamax® and Ginger Oil Potential Neurotoxicity And Their Interaction in Mice Thru Oxidative Stress and Inflammatory Markers, Neurotransmitters Expression and Immunohistochemical Measurements
Authors: Mabrouk Dalia M , makawy Aida El , Ahmed Kawkab A , Ibrahim Faten M 
Published: January 2022

Abstract
Background: Topamax® ® has multiple pharmacological mechanisms that are efficient to treat epilepsy and migraine. Ginger has been demonstrated to have gingerols and shogaols compounds that proven to cross the blood-brain barrier causing migraine relief, implying that it is useful in the treatment of migraines. Moreover, Topamax has many off-label uses. So it was necessary to explore the possible neurotoxicity of Topamax®, Ginger oil and their interaction in the mice brain. Methods and Results: Male mice were orally gavage with Topamax®, ginger oil (400mg/kg), and Topamax® plus ginger oil with the same pattern for one month. Oxidative stress markers, acetylcholinesterase (AchE) and gamma aminobutyric acid (GABA) and tumor necrosis factor-alpha (TNF- α), were analyzed in brain tissue. Histopathological examination by hematoxylin and eosin, immunohistochemical glial fibrillary acidic protein (GFAP), and Bax expression analysis were done. The mRNA levels of GABAAR subunits, Gabra1, Gabra3, and Gabra5 were evaluated by RT qPCR. The analysis of data revealed that Topamax® elevated the levels of oxidative stress markers, neurotransmitters, TNF-α, and diminished the level of glutathione reduced (GSH). Topamax® exhibited various neuropathological alterations, strong Bax expression, and GFAP immune-reactivity in the cerebral cortex. The interaction effect of Topamax® plus ginger oil attenuated the changes induced by Topamax® in the abovementioned parameters. Both Topamax® and ginger oil upregulated the mRNA expression of gabra1 and gabra3 while their interaction markedly downregulated them. Conclusion: We can conclude that the Topamax® overdose could possibly cause neurotoxicity, but the interaction with ginger oil can reduce Topamax® -induced neurotoxicity and should be taken in parallel.
Study on Motion Management of Lung Cancer Treated by CyberKnife: Based on Tumor Position
Authors: Jing Shenghua , Wang Zhen , Jiang Changchen , Qiu Xiangnan , Wu Taincong , Zhang Yan , Li Yikun , Wang Wei , Sun Xiangdong 
Published: January 2022

Abstract
Purpose: We investigated the movement characteristics of lung cancers and the clinical accuracy of tracking lung tumors with Synchrony Respiratory Tracking System (SRTs) during the CyberKnife treatment. We also explored the influencing factors of accuracy. These data provided the appropriate expansion margins of patients with different respiratory characteristics, which was helpful to realize the personalized design of treatment plans of CyberKnife. Methods and Materials: 73 patients with lung cancer treated with CyberKnife SRTs were selected retrospectively for this study. The patient's age, gender, respiratory characteristics and tumor datas (tumor size, anatomical position and geometric position) were recorded. During treatment, the deviation was checked every 45 s and compensated by the synchronous respiratory tracking system.Results: The total mean motion amplitudes and standard deviations of lung tumors in superior-inferior (SI), left-right (LR), and anterior-posterior (AP) directions were 4.15 ± 3.47 mm, 3.98 ± 3.21 mm and 3.79 ± 2.73 mm, respectively. The overall mean correlation errors and standard deviations were 0.86 ± 0.45 mm, 1.04 ± 0.76 mm and 0.70 ± 0.47 mm, respectively. The overall mean prediction errors and standard deviations were 0.18 ± 0.17 mm, 0.35 ± 0.39 mm and 0.35 ± 0.42 mm, respectively. The correlation errors of LR direction were less correlated with the geometric position of the tumor (r = 0.38), and not correlated with the anatomical position of the tumor (r < 0.3). The prediction errors were moderately correlated with the respiratory amplitude (r = 0.588), and less correlated with the baseline drift and the motion amplitude of the tumor (r = 0.407 and 0.365, respectively).Conclusions: The patient’s respiratory amplitude, the tumor motion amplitude, the tumor baseline drift and geometric position were the main factors affecting the tracking accuracy. Tumors at different geometric positions should be treated differently to ensure sufficient dose coverage of the lung tumor target.
Heterogeneity of Soil Bacterial and Bacteriophage Communities in Three Rice Agroecosystems and Potential Impacts On Nutrient Cycling
Authors: Wang Yajiao , Liu Yu , Wu Yuxing , Wu Nan , Liu Wenwen , Wang Xifeng 
Published: January 2022

Abstract
Background: As genetic entities infecting and replicating only in bacteria, bacteriophages can regulate the community structure and functions of their host bacteria, but they are often overlooked because of their relatively low abundance. The ecological roles of bacteriophages in aquatic and forest environments have been widely explored, but those in agroecosystems remains limited. Here, we used metagenomic sequencing to analyze the diversity and interactions of bacteriophages and their host bacteria in soils from three typical rice agroecosystems in China: double cropping in Guangzhou, southern China, rice–wheat rotation cropping in Nanjing, eastern China and early maturing single cropping in Jiamusi, northeastern China. Bacteriophages were isolated and their functions on soil nitrogen cycling and effect on soil bacterial community structure were verified in pot inoculation experiments and Illumina MiSeq sequencing.Results: Soil bacterial and viral diversity and functions varied among the three agroecosystems. Genes detected in communities from the three agroecosystems were associated with typical functions; soil bacteria in Jiamusi were significantly enriched in genes related to carbohydrate metabolism, in Nanjing with xenobiotics biodegradation and metabolism, and in Guangzhou with virulence factors and scarce in secondary metabolite biosynthesis, which might lead to a significant occurrence of rice bacterial diseases. In the three ecosystems, 368 species of virus were detected. Notably, over-represented auxiliary carbohydrate-active enzyme (CAZyme) genes were identified in the viruses, which might assist host bacteria in metabolizing carbon, and 67.43% of these genes were present in Jiamusi. In bacteriophage isolation and inoculation experiments, Enterobacter bacteriophage-NJ reduced the nitrogen fixation capacity of soil by lysing N-fixing host bacteria and changed the soil bacterial diversity and community structure.Conclusions: Our results showed that diversity and function of paddy soil bacteria and viruses varied in the three agroecosystems. Soil bacteriophages can affect nutrient cycling by expressing auxiliary metabolic genes (AMGs) and lysing the host bacteria that are involved in biogeochemical cycles. These findings form a basis for better understanding bacterial and bacteriophage diversity in different rice agroecosystems, laying a solid foundation for further studies of soil microbial communities that support ecofriendly production of healthy rice.
Fast Dispersive Liquid-Liquid Microextraction Based on Temperature Switching Deep Eutectic Solvent as A Green Extractant
Authors: Liu Qian , Li Zhiqiang , Wei Liulin , Chen Xiaomei , Xu Yidong , Zhao Jingchan 
Published: January 2022

Abstract
With the development of research, it was found that the dispersive liquid-liquid microextraction (DLLME) was limited by the tedious extraction process and toxic extractant. A thermo-switchable deep eutectic solvent (DES) was applied to the DLLME as a green extractant in this paper. The DES can be dispersed and aggregated by shaking and heating in the water during the extraction process due to the surfactant and thermo-switchable properties, which shortened the extraction time as low as 5 minutes. The feasibility of the developed method was verified using 4 organophosphorus pesticides and 5 pyrethroids as targets in simulated water samples, which showed good precision (RSD%, 0.8-9.7, n=3) and low detection limit (0.16-0.81 µg L−1, S/N = 3) under the optimal extraction conditions. This method was used to detect the pesticide content in four natural rivers in Xi'an, and the recoveries of these spiked samples at three concentrations levels were among 81–113%. This is the first time to combine the toxic-free and thermo-switchable properties of DES in DLLME to proposes a simple, fast, effective, and green pretreatment method.
RAB27A Promotes the Proliferation and Invasion of Colorectal Cancer Cells
Authors: Li Qingyan , Zhao Huixia , Dong Weiwei , Guan Na , Hu Yanyan , Zeng Zhiyan , Zhang He , Zhang Fengyun , Li Qiuwen , Yang Jingwen , Xiao Wenhua 
Published: January 2022

Abstract
Colorectal cancer (CRC) is the most commonly diagnosed form of cancer worldwide. Though significant advances in prevention and diagnosis, CRC is still one of the leading causes of cancer-related mortality globally. RAB27A, the member of RAB27 family of small GTPases, is the critical protein for intracellular secretion and was reported to promote tumor progression. However, it is controversial for the role of RAB27A in CRC progression, so we explored the exact function of RAB27A in CRC development in this study. Based on the stable colon cancer cell lines of RAB27A knockdown and ectopic expression, we found that RAB27A knockdown inhibited SW480 colon cancer cell proliferation and clone formation, whereas ectopic expression of RAB27A in RKO colon cancer cells facilitated cell proliferation and clone formation, indicating that RAB27A is critical for colon cancer cell growth. In addition, our data demonstrated that the migration and invasion of colon cancer cells were suppressed by RAB27A knockdown, but promoted by RAB27A ectopic expression. Therefore, RAB27A was identified as an onco-protein in mediating CRC development, which may be a valuable prognostic indicator and potential therapeutic target for CRC.
Spatial Pattern and Influencing Factors of Intangible Cultural Heritage of Music in Xiangxi, Central China
Authors: zhang xian yong , xiang hui , Liu Rui 
Published: January 2022

Abstract
Intangible cultural heritage of music (ICHM) is an important part of intangible cultural heritage (ICH), and research on ICHM has extremely important theoretical value and practical significance. In this study, we aimed to analyze the spatial distribution characteristics of ICHMs and explore the main factors affecting their distribution in Xiangxi, Central China. Methods such as the nearest neighbor index, kernel density estimation, and buffer analysis were used in this study. The main conclusions are as follows: 1) the numbers of traditional music, traditional dance, traditional drama and quyi are 33, 34, 30 and 14, respectively, indicating that quyi is relatively lacking in Xiangxi; 2) the distribution of ICHMs in Xiangxi have spatial heterogeneity, and the main trend is agglomeration. The national ICHMs, provincial ICHMs and municipal ICHMs are mainly concentrated in the south, the west, and the central and northern regions, respectively. The high-density areas of traditional music and traditional dance are located in the south, of traditional drama in the west, and of quyi in the north. 3) The distribution of ICHMs is affected by four factors: namely rivers, topography, GDP and transportation. The 0-3 km buffer zone of the rivers, with 100% of national ICHMs, 83.33% of provincial ICHMs and 84.52% of municipal ICHMs, is the centralized distribution area of ICHMs. 100% of the national ICHMs, 94.44% of the provincial ICHMs and 91.67% of the municipal ICHMs are distributed in hilly (200-500 m) and low mountain areas (500-1000 m). Highways have the greatest impact on the distribution of ICHMs, followed by railways and ordinary roads. The economy has a significant positive impact on ICHM programs; the more developed the economy, the more ICHM projects. The results of this study can provide useful references for the theoretical research and practice management of ICH.
SHP-2 and canonical PD-1: SHP-2 axis regulate myeloid cell differentiation, anti-tumor responses and innate immune memory
Authors: Boussiotis Vassiliki , Christofides Anthos , Katopodi Xanthi-Lida , Cao Carol , Aksoylar Halil-Ibrahim , Mahmoud Mohamed , Strauss Laura , Tijaro-Ovalle Natalia , Pal Rinku , Boon Louis , Asara John , Vlachos Ioannis , Patsoukis Nikolaos 
Published: January 2022

Abstract
PD-1 checkpoint inhibitor induces T cell inactivation by recruiting SHP-2. However, T cell-specific SHP-2-deficient mice do not have improved anti-tumor immunity. We generated mice with conditional targeting of the Ptpn11 gene (encoding for Shp-2) in T cells (Shp2f/fLckCre) or myeloid cells (Shp2f/fLysMCre), and found that Shp2f/fLysMCre mice had diminished tumor growth. As determined by RNA-seq, this was paralleled by the presence of inflammatory neutrophils and tumor-associated macrophages (TAMs) with molecular signatures of enhanced differentiation, phagocytosis and antigen-processing and presentation. SHP-2 deficient TAMs also had increased monocyte and dendritic cell (DC) specification transcription factors, chemokine and cytokine production, and expression of immunostimulatory molecules that promote T cell recruitment and activation. Monocytes from tumor-bearing Shp2f/fLysMCre mice suppressed tumor growth after transfer to naïve recipients indicating development of innate immune memory. In bone marrow myelocytes, GM-CSF, induced PD-1 expression, phosphorylation and interaction with SHP-2, the Src family kinase Lyn, and GM-CSF receptor beta chain, indicating that the PD-1:SHP-2 axis targets a key pathway of myelocyte differentiation. In contrast, SHP-2 deletion or antibody-mediated blockade of the PD-1:PD-L1 pathway enhanced phosphorylation of the transcription factors HOXA10 and IRF8 that regulate myeloid differentiation and monocytic/moDC lineage commitment, respectively. Thus, SHP-2 and the PD-1:SHP-2 axis pose a signaling restrain to myeloid differentiation and monocyte lineage commitment resulting in a myeloid landscape that suppresses anti-tumor immunity.
Investigating the Effect of an Online Self-Care Training Program on Perceived Stress in COVID-19 Patients
Authors: Marzban Arash , Farsi Zahra , Afaghi Effat , Rezaei Mehdi , Moradi Mohsen 
Published: January 2022

Abstract
Background: The coronavirus disease 2019 (COVID-19) caused many adverse effects, including increased stress in patients. The present study aimed to determine the effect of an online self-care training program on perceived stress in COVID-19 patients.Methods: This quasi-experimental study was fulfilled by recruiting 132 COVID-19 patients, referred to two hospitals in Isfahan, Iran, from February 20 to August 22, 2021. These patients were selected using the convenience sampling method, and were then randomized into experimental (n=63) and control (n=63) groups. The online self-care training program was subsequently presented to the patients in the experimental group, during six sessions of 30 minutes for two weeks. E-learning and educational content introduced through lectures, multimedia, and instructional videos using web platforms. Then, daily follow-up sessions continued until one month after the intervention. The data were collected administering an individual characteristic form and the Perceived Stress Scale (PSS) at three stages before, immediately, and one month after the intervention.Results: The mean scores of perceived stress before, immediately, and one month after the intervention in the experimental group were respectively 30.51±6.31, 24.59±4.66, and 26.57±3.82, and they were 29.78±4.81, 29.4±4.57, and 29.11±4.73 in that order in the control group. Moreover, no significant difference was observed between both groups at the pre-intervention stage (p=0.467), while the mean scores of perceived stress in the experimental group were lower compared with those in the control group immediately (p<0.001) and one month after the intervention (p=0.001). The mean scores of perceived stress in the experimental group before, immediately, and one month after the intervention had also changed in a significant manner (p<0.001), whereas there was no significant variation in the control group in this respect (p=0.298).Conclusions: The online self-care training program led to a reduction in perceived stress in COVID-19 patients. It is thus recommended to implement this cheap, safe, and efficient method to relieve perceived stress in such cases.
SELINA: Single-cell Assignment using Multiple-Adversarial Domain Adaptation Network with Large-scale References
Authors: Wang Chenfei , Ren Pengfei , Shi Xiaoying , Dong Xin , Yu Zhiguang , Ding Xuanxin , Wang Jin , Sun Liangdong , Yan Yilv , Hu Junjie , Zhang Peng , Chen Qianming , Li Taiwen 
Published: January 2022

Abstract
The rapid accumulation of single-cell RNA-seq data has provided rich resources to characterize various human cell types. Cell type annotation is the critical step in analyzing single-cell RNA-seq data. However, accurate cell type annotation based on public references is challenging due to the inconsistent annotations, batch effects, and poor characterization of rare cell types. Here, we introduce SELINA (single cELl identity NAvigator), an integrative annotation transferring framework for automatic cell type annotation. SELINA optimizes the annotation for minority cell types by synthetic minority over-sampling, removes batch effects among reference datasets using a multiple-adversarial domain adaptation network (MADA), and fits the query data with reference data using an autoencoder. Finally, SELINA affords a comprehensive and uniform reference atlas with 1.7 million cells covering 230 major human cell types. We demonstrated the robustness and superiority of SELINA in most human tissues compared to existing methods. SELINA provided a one-stop solution for human single- cell RNA-seq data annotation with the potential to extend for other species.
Pioneering Evaluation of GaN Transistors in Geostationary Satellites
Authors: Carvalho Nuno , Mostardinha Hugo , Matos Diogo , Sampaio Jorge , Pinto Marco , Gonçalves Patricia , Sousa Tiago , Kurpas Paul , Wuerfl Joachim , Barnes Andrew , Garat François , Poivey Christian 
Published: January 2022

Abstract
In this paper, we present the results of a 6-year experiment in space that studied the effects of radiation in GaN electronics in geostationary orbit. Four GaN transistors in a Colpitts oscillator configuration were flown in the Component Technology Test-Bed aboard the Alphasat telecommunication satellite. A heuristic analysis was performed by observing the variation in the power output of the oscillators with the total ionizing dose gathered during the mission. The total ionizing dose was measured with a RADFET placed close to the GaN devices. The experiment showed that GaN is a robust technology that can be used in the space radiation environment of a geostationary orbit. The work presented here starts with a brief introduction of the subject, the motivation and the main goal. This is followed by the description of the experimental setup, including the details of the oscillator design and simulations, as well as the implementation of the test bead and the Components Technology Test Bed. Finally, the results obtained during the 6 years of experience in space are discussed.