bluuebunny/crossref_metadata_2025_split huggingface dataset:
A single application of cold atmospheric plasma (CAP) improves blood flow parameters in chronic wounds
Authors: Schleusser Sophie , Schulz Lysann , Song Jungin , Deichmann Henriette , Griesmann Anna‐Catharina , Stang Felix H. , Mailaender Peter , Kraemer Robert , Kleemann Markus , Kisch Tobias 
Published: February 2022

AbstractObjectiveTo find out whether application of cold atmospheric plasma (CAP) affects microcirculation in chronic wounds.MethodsWe treated 20 patients with chronic wounds on the lower extremity with CAP. Blood flow parameters of wounds were assessed with combined Laser‐Doppler‐Flowmetry and spectrophotometry in tissue depth of 2 and 6–8 mm. Parameters were assessed under standardized conditions before and over the course of 30 min after application of CAP.ResultsDeep capillary blood flow increased significantly by up to 24.33% (percentage change) after treatment with CAP and remained significantly elevated until the end of measuring period at 30 min. Superficial oxygen tissue saturation was significantly elevated by 14.05% for the first 5 min after treatment. Postcapillary venous filling pressure was significantly elevated by 10.23% 19 min after CAP and stayed significantly elevated starting from minute 24 until the end of measuring.ConclusionCold atmospheric plasma increases microcirculation parameters in chronic wounds significantly. As CAP is known for its benefits in wound healing, the effects observed may explain the improved healing of chronic wounds after its use. Whether CAP‐application can increase blood flow in chronic wounds for longer periods of time or boosts blood flow when applied more than once should be subject to further research.
Origen’s Johannine Trinitarian Theology of Love
Authors: Ip Pui Him 
Published: February 2022

AbstractOrigen is the first Christian who proposed a systematically Trinitarian theology of love. This has largely escaped the attention of theologians and remains underexplored. One notable consequence is that this has severely limited our appreciation of Origen as a significant interlocutor for contemporary theology since the Trinity as love is arguably the most significant theme that shaped the development of modern Trinitarian theology in the twentieth century. This essay addresses this lacuna by offering a reconstruction of Origen’s Trinitarian theology of love. What will emerge is a grammar of love that sets out the meaning of the term with reference to all three divine hypostaseis, Father, Son, and Holy Spirit. Furthermore, Origen’s Trinitarian understanding of love is bound up with his vision of human deification understood as incorporation into the Triune fellowship of love. At the heart of Origen’s approach is a crucial distinction from 1 John: the Father as he agapē (1 John 4:8) and the Son as he agapē ek tou theou (1 John 4:7). This distinction captures Origen’s understanding of the Father‐Son relation and emerges from close attention to the Johannine language of sonship as ek tou theou. This central observation urges a reassessment of subordinationism as a characterisation of Origen’s Trinitarian thought. I argue that it is more appropriate instead to speak of Origen’s Johannine theology of sonship.
High‐throughput measurement of plant fitness traits with an object detection method using Faster R‐CNN
Authors: Wang Peipei , Meng Fanrui , Donaldson Paityn , Horan Sarah , Panchy Nicholas L. , Vischulis Elyse , Winship Eamon , Conner Jeffrey K. , Krysan Patrick J. , Shiu Shin‐Han , Lehti‐Shiu Melissa D. 
Published: February 2022

Summary

Revealing the contributions of genes to plant phenotype is frequently challenging because loss‐of‐function effects may be subtle or masked by varying degrees of genetic redundancy. Such effects can potentially be detected by measuring plant fitness, which reflects the cumulative effects of genetic changes over the lifetime of a plant. However, fitness is challenging to measure accurately, particularly in species with high fecundity and relatively small propagule sizes such as Arabidopsis thaliana.

An image segmentation‐based method using the software ImageJ and an object detection‐based method using the Faster Region‐based Convolutional Neural Network (R‐CNN) algorithm were used for measuring two Arabidopsis fitness traits: seed and fruit counts.

The segmentation‐based method was error‐prone (correlation between true and predicted seed counts, r2 = 0.849) because seeds touching each other were undercounted. By contrast, the object detection‐based algorithm yielded near perfect seed counts (r2 = 0.9996) and highly accurate fruit counts (r2 = 0.980). Comparing seed counts for wild‐type and 12 mutant lines revealed fitness effects for three genes; fruit counts revealed the same effects for two genes.

Our study provides analysis pipelines and models to facilitate the investigation of Arabidopsis fitness traits and demonstrates the importance of examining fitness traits when studying gene functions.
‘Sono solo parole’: Facing challenges entailed in developing and applying terminologies to document nursing care
Authors: Malabusini Cecilia 
Published: February 2022

AbstractNurses’ need to document activities is urgent. The panorama of available terminologies is heterogeneous. It seems necessary to understand the premises of available tools and their limits and benefits to make conscious choices and shape future development. Taxonomies (e.g., North American Nursing Diagnosis Association) and ‘pure terminologies’ (e.g., International Classification for Nursing Practice), or nursing languages, are available tools to document nurses’ activities and to produce theoretical models or reference systems. These tools respond first to a practical problem: ‘translating’ nursing facts into linguistic descriptions. Taxonomies make reality describable through exhaustive definitions designed inductively. Thus, I argue that their philosophical basis is naïve semantics. Their framework is a closed hierarchy, that is, a system that provides defined levels of concepts, structured through dichotomous rules. This causes the problem of determining boundaries between concepts, and thus between phenomena, which I call the problem of ‘closing the concept enclosure’. Rigid metaphysical structures are unable to cope with the complexity of phenomena that nurses deal with, so they seem to be unable to describe them. I follow Wittgenstein's argument, from Philosophical Investigations, which is useful to understand this challenge: the definition of terms is not a problem of lack of suitable words or an agreed decision, but is achieved by training in practice. Pure terminologies give us another way to address the question. They are usually composed of a glossary of terms, paired with rules to combine them to manage meaning. They are more like natural language and this implies adapting more to phenomena. Then, I argue that their philosophical background is a pragmatic approach to language, and I discuss some consequences and objections. Finally, I find that the development of pure terminologies rather than taxonomies in documenting nursing activities allows us to achieve key objectives, such as improving nursing visibility and measurability in health records, without jeopardising person‐centred care.
Role of lncRNAs in cis‐ and trans‐regulatory responses to salt in Populus trichocarpa
Authors: Ye Xiaoxue , Wang Shuo , Zhao Xijuan , Gao Ni , Wang Yao , Yang Yanmei , Wu Ernest , Jiang Cheng , Cheng Yuxiang , Wu Wenwu , Liu Shenkui 
Published: February 2022

SUMMARYLong non‐coding RNAs (lncRNAs) are emerging as versatile regulators in diverse biological processes. However, little is known about their cis‐ and trans‐regulatory contributions in gene expression under salt stress. Using 27 RNA‐seq data sets from Populus trichocarpa leaves, stems and roots, we identified 2988 high‐confidence lncRNAs, including 1183 salt‐induced differentially expressed lncRNAs. Among them, 301 lncRNAs have potential for positively affecting their neighboring genes, predominantly in a cis‐regulatory manner rather than by co‐transcription. Additionally, a co‐expression network identified six striking salt‐associated modules with a total of 5639 genes, including 426 lncRNAs, and in these lncRNA sequences, the DNA/RNA binding motifs are enriched. This suggests that lncRNAs might contribute to distant gene expression of the salt‐associated modules in a trans‐regulatory manner. Moreover, we found 30 lncRNAs that have potential to simultaneously cis‐ and trans‐regulate salt‐responsive homologous genes, and Ptlinc‐NAC72, significantly induced under long‐term salt stress, was selected for validating its regulation of the expression and functional roles of the homologs PtNAC72.A and PtNAC72.B (PtNAC72.A/B). The transient transformation of Ptlinc‐NAC72 and a dual‐luciferase assay of Ptlinc‐NAC72 and PtNAC72.A/B promoters confirmed that Ptlinc‐NAC72 can directly upregulate PtNAC72.A/B expression, and a presence/absence assay was further conducted to show that the regulation is probably mediated by Ptlinc‐NAC72 recognizing the tandem elements (GAAAAA) in the PtNAC72.A/B 5′ untranslated region (5′‐UTR). Finally, the overexpression of Ptlinc‐NAC72 produces a hypersensitive phenotype under salt stress. Altogether, our results shed light on the cis‐ and trans‐regulation of gene expression by lncRNAs in Populus and provides an example of long‐term salt‐induced Ptlinc‐NAC72 that could be used to mitigate growth costs by conferring plant resilience to salt stress.
The adaptive evolution of Euryale ferox to the aquatic environment through paleo‐hexaploidization
Authors: Wu Peng , Zhang Lingkui , Zhang Kang , Yin Yulai , Liu Ailian , Zhu Yue , Fu Yu , Sun Fangfang , Zhao Shuping , Feng Kai , Xu Xuewen , Chen Xuehao , Cheng Feng , Li Liangjun 
Published: February 2022

SUMMARYOccupation of living space is one of the main driving forces of adaptive evolution, especially for aquatic plants whose leaves float on the water surface and thus have limited living space. Euryale ferox, from the angiosperm basal family Nymphaeaceae, develops large, rapidly expanding leaves to compete for space on the water surface. Microscopic observation found that the cell proliferation of leaves is almost completed underwater, while the cell expansion occurs rapidly after they grow above water. To explore the mechanism underlying the specific development of leaves, we performed sequences assembly and analyzed the genome and transcriptome dynamics of E. ferox. Through reconstruction of the three sub‐genomes generated from the paleo‐hexaploidization event in E. ferox, we revealed that one sub‐genome was phylogenetically closer to Victoria cruziana, which also exhibits gigantic floating leaves. Further analysis revealed that while all three sub‐genomes promoted the evolution of the specific leaf development in E. ferox, the genes from the sub‐genome closer to V. cruziana contributed more to this adaptive evolution. Moreover, we found that genes involved in cell proliferation and expansion, photosynthesis, and energy transportation were over‐retained and showed strong expression association with the leaf development stages, such as the expression divergence of SWEET orthologs as energy uploaders and unloaders in the sink and source leaf organs of E. ferox. These findings provide novel insights into the genome evolution through polyploidization, as well as the adaptive evolution regarding the leaf development accomplished through biased gene retention and expression sub‐functionalization of multi‐copy genes in E. ferox.
Ryanodine receptor activity and store‐operated Ca2+ entry: Critical regulators of Ca2+ content and function in skeletal muscle
Authors: Pearce Luke , Meizoso‐Huesca Aldo , Seng Crystal , Lamboley Cedric R. , Singh Daniel P. , Launikonis Bradley S. 
Published: February 2022

AbstractStore‐operated Ca2+ entry (SOCE) is critical to cell function. In skeletal muscle, SOCE has evolved alongside excitation–contraction coupling (EC coupling); as a result, it displays unique properties compared to SOCE in other cells. The plasma membrane of skeletal muscle is mostly internalized as the tubular system, with the tubules meeting the sarcoplasmic reticulum (SR) terminal cisternae, forming junctions where the proteins that regulate EC coupling and SOCE are positioned. In this review, we describe the properties and roles of SOCE based on direct measurements of Ca2+ influx during SR Ca2+ release and leak. SOCE is activated immediately and locally as the [Ca2+] of the junctional SR terminal cisternae ([Ca2+]jSR) depletes. [Ca2+]jSR changes rapidly and steeply with increasing activity of the SR ryanodine receptor isoform 1 (RyR1). The high fidelity of [Ca2+]jSR with RyR1 activity probably depends on the SR Ca2+‐buffer calsequestrin that is located immediately behind RyR1 inside the SR. This arrangement provides in‐phase activation and deactivation of SOCE with a large dynamic range, allowing precise grading of SOCE flux. The in‐phase activation of SOCE as the SR partially depletes traps Ca2+ in the cytoplasm, preventing net Ca2+ loss. Mild presentation of RyR1 leak can occur under physiological conditions, providing fibre Ca2+ redistribution without changing fibre Ca2+ content. This condition preserves normal contractile function at the same time as increasing basal metabolic rate. However, higher RyR1 leak drives excess cytoplasmic and mitochondrial Ca2+ load, setting a deleterious intracellular environment that compromises the function of the skeletal muscle.
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Muscle hypertrophic effect of inhaled beta2‐agonist is associated with augmented insulin‐stimulated whole‐body glucose disposal in young men
Authors: Jessen Søren , Baasch‐Skytte Thomas , Onslev Johan , Eibye Kasper , Backer Vibeke , Bangsbo Jens , Hostrup Morten 
Published: February 2022

AbstractRodent studies highlight enhancement of glucose tolerance and insulin sensitivity as potential clinically relevant effects of chronic beta2‐agonist treatment. However, the doses administered to rodents are not comparable with the therapeutic doses used for humans. Thus, we investigated the physiological effects of prolonged beta2‐agonist treatment at inhaled doses resembling those used in respiratory diseases on insulin‐stimulated whole‐body glucose disposal and putative mechanisms in skeletal muscle and adipose tissue of healthy men. Utilizing a randomized placebo‐controlled parallel‐group design, we assigned 21 healthy men to 4 weeks daily inhalation of terbutaline (TER; 4 mg × day−1, n = 13) or placebo (PLA, n = 8). Before and after treatments, we assessed subjects’ whole‐body insulin‐stimulated glucose disposal and body composition, and collected vastus lateralis muscle and abdominal adipose tissue biopsies. Glucose infusion rate increased by 27% (95% CI: 80 to 238 mg × min−1, P = 0.001) in TER, whereas no significant changes occurred in PLA (95% CI: −37 to 195 mg × min−1, P = 0.154). GLUT4 content in muscle or adipose tissue did not change, nor did hexokinase II content or markers of mitochondrial volume in muscle. Change in lean mass was associated with change in glucose infusion rate in TER (r = 0.59, P = 0.03). Beta2‐agonist treatment in close‐to‐therapeutic doses may augment whole‐body insulin‐stimulated glucose disposal in healthy young men and part of the change is likely to be explained by muscle hypertrophy. These findings highlight the therapeutic potential of beta2‐agonists for improving insulin sensitivity.Key points
While studies in rodents have highlighted beta2‐agonists as a means to augment insulin sensitivity, these studies utilized beta2‐agonists at doses inapplicable to humans.
Herein we show that a 4‐week treatment period with daily therapeutic inhalation of beta2‐agonist increases insulin‐stimulated whole‐body glucose disposal in young healthy lean men.
This effect was associated with an increase of lean mass but not with changes in GLUT4 and hexokinase II or basal glycogen content in skeletal muscle nor GLUT4 content in abdominal adipose tissue.
These findings suggest that the enhanced insulin‐stimulated whole‐body glucose disposal induced by a period of beta2‐agonist treatment in humans, at least in part, is attributed to muscle hypertrophy.
Our observations extend findings in rodents and highlight the therapeutic potential of beta2‐agonists to enhance the capacity for glucose disposal and whole‐body insulin sensitivity, providing important knowledge with potential application in insulin resistance.
Phase‐specific pooling of sparse assembly activity by respiration‐related brain oscillations
Authors: Folschweiller Shani , Sauer Jonas‐Frederic 
Published: February 2022

AbstractNasal breathing affects cognitive functions, but it has remained largely unclear how respiration‐driven inputs shape information processing in neuronal circuits. Current theories emphasize the role of neuronal assemblies, coalitions of transiently active pyramidal cells, as the core unit of cortical network computations. Here, we show that the phase of respiration‐related oscillations (RROs) influences the likelihood of activation of a subset of neuronal assemblies in the medial prefrontal cortex of awake mice. RROs bias the activation of neuronal assemblies more efficiently than that of individual neurons by entraining the coactivity of assembly neurons. Moreover, the activation of assemblies is moderately biased towards the descending phase of RROs. Despite the enriched activation of assemblies during descending RRO, the overlap between individual assemblies remains constant across RRO phases. Putative GABAergic interneurons are shown to coactivate with assemblies and receive enhanced excitatory drive from assembly neurons during descending RRO, suggesting that the phase‐specific recruitment of putative interneurons might help to keep the activation of different assemblies separated from each other during times of preferred assembly activation. Our results thus identify respiration‐synchronized brain rhythms as drivers of neuronal assemblies and point to a role of RROs in defining time windows of enhanced yet segregated assembly activity.Key points
Activation of neuronal assemblies is phase‐coupled to ongoing respiration‐related oscillations (RROs) in the medial prefrontal cortex of mice.
The phase coupling strength of assemblies exceeds that of individual neurons.
Assemblies preferentially activate during the descending phase of RRO.
Despite higher assembly frequency during descending RRO, overlap between active assemblies remains constant across RRO phase.
Putative GABAergic interneurons are preferentially recruited by assembly neurons during descending RRO, suggesting that interneurons might contribute to the segregation of active assemblies during the descending phase of RRO.
Spanish Adaptation of the Groningen Sleep Quality Scale (GSQS-8)
Authors: Serrano-Fernández María-José , Boada-Grau Joan , Robert-Sentís Lluís , Boada-Cuerva Maria , Vigil-Colet Andreu , Assens-Serra Jordi 
Published: February 2022

Professional drivers are a group susceptible to sleep problems or incorrect rest patterns resulting from work stress that causes alterations in biological stress markers, such as cortisol, or in cardiovascular parameters that show a state of physiological hyper-activation. The current research objective was to adapt and validate the Groningen Sleep Scale (Meijman et al., 1990) in a Spanish population. We analysed its internal structure, reliability and evidence of validity. The participants in this study were 372 drivers (93.4% men, 6.6% women), with a mean age of 40.9 (SD = 10.54), obtained through non-probabilistic sampling. The SPSS 23.0 and AMOS (5.0) programs were used. With the confirmatory factor analysis (CFA) of the AMOS program (5.0), the indicators NFI = 0.902; TLI = 0.844; CFI = 0.913; RMSEA = 0.129 were obtained and showed an acceptable adjustment of the unifactorial model with 8 items. Adequate reliability (0.90) and appropriate evidence of validity with TDS-38, MBI-GS, Irritation, MP-9, DII, and Trans-18. We can conclude that the Groningen Sleep Scale (GSQS-8) is a reliable and valid instrument, suitable in the Spanish language for evaluating the sleep quality of professional drivers.
Barreras y factores de éxito percibidos por el profesorado universitario. Diferencias por género y categorías académicas
Authors: Gallego Morón Nazareth , Matus-López Mauricio , Gálvez Muñoz Lina 
Published: February 2022

Este artículo explora en qué medida los factores personales, organizacionales y sociales determinan las trayectorias del profesorado universitario, controlando variables sociodemográficas como sexo y rango académico. Para ello, se desarrolló un estudio de tipo correlacional, en una muestra de profesorado de la Universidad Federico II de Nápoles, en Italia (N = 228, 55.3 % hombres y 44.7 % mujeres), de entre 21 y 67 años (M = 51.7, DE = 9.2), con el empleo de estadística descriptiva y análisis de correspondencia múltiple. Los hallazgos evidencian dos tendencias diferenciadas: (1) la del profesorado masculino y de mayor nivel y (2) la de las mujeres y el profesorado de categorías medias e inferiores. Los primeros perciben menos dificultades y valoran más los factores de éxito personales (Modelo 1, 53.9 % de la varianza) y familiares (Modelo 2, 50.3 % de la varianza). Barreras como la influencia de redes informales de poder (Modelo 3, 42.7 % de la varianza) y cuestiones familiares, como la dificultad de conciliación familiar, personal y laboral (Modelos 4 y 5, 50.2 % y 59.9 % de la varianza), son percibidos con mayor intensidad por el segundo grupo. A raíz de estos datos, se destaca la importancia de medidas en pro de la igualdad de oportunidades en la academia.
Evaluación del sentido de comunidad y su variación según las características del contexto geográfico costarricense
Authors: Von Breymann Helga 
Published: February 2022

El constructo sentido de comunidad ha adquirido gran relevancia en los estudios urbanos por su asociación con una mejora en la calidad de vida, eficacia colectiva y satisfacción residencial. Este estudio buscó evaluar la estabilidad del constructo de sentido de comunidad en un contexto latinoamericano a partir de la escala de MTSOCS (Prezza et al., 2009), al igual que identificar asociaciones potenciales entre las variables que mide la escala y la distribución geográfica. Se realizó un estudio de carácter exploratorio transversal a una muestra de 394 residentes (M Edad = 43.6, DE Edad = 16.1) del cantón Flores, en Costa Rica. La recolección de datos se realizó aplicando un cuestionario en un procedimiento puerta a puerta. Los resultados del Análisis Factorial Confirmatorio aportan evidencias de validez de la escala MTSOCS. Adicionalmente, los resultados de los mapas con la distribución geográfica de los datos muestran la presencia de patrones asociados a las características físico-espaciales del territorio. Lo anterior confirma la asociación de los atributos urbanos con el sentido de comunidad de la población y la importancia de combinar la escala con sistemas de información geográfica para mejorar la comprensión del constructo y su aplicación en la planeación urbana.