bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pankreaszysten: nicht gesucht, aber gefunden – was nun?
Authors: Heinrich Henriette , Kahl Stefan 
Published: September 2022

ZusammenfassungZystische Pankreasläsionen und zystische Pankreasneoplasien (PCN) haben mit bis zu 49 % eine hohe Prävalenz in der Allgemeinbevölkerung. Bei einer Pankreaskarzinominzidenz von 7,6/100.000 in der Allgemeinbevölkerung in Europa wird deutlich, dass nur ein Bruchteil der Patienten mit PCN langfristig ein Karzinom entwickeln wird. Die Zystengrösse, Verbindung zum Hauptgang sowie die Dilatation des Pankreasgangs sind zusammen mit der Analyse der Zystenflüssigkeit wichtige, jedoch nicht ausreichend sensitive und spezifische Marker zur Bestimmung der Dignität von PCN. Aktuell fehlen weiterhin verlässliche Biomarker zur sicheren Risikostratifizierung zystischer Pankreasläsionen, was die Durchführung einer Surveillance bei Patienten mit PCN, solange eine Operabilität besteht, unerlässlich macht.
NEW LOSS RESERVE MODELS WITH PERSISTENCE EFFECTS TO FORECAST TRAPEZOIDAL LOSSES IN RUN-OFF TRIANGLES
Authors: Usman Farha , Chan Jennifer S.K. 
Published: September 2022

AbstractModelling loss reserve data in run-off triangles is challenging due to the complex but unknown dynamics in the claim/loss process. Popular loss reserve models describe the mean process through development year, accident year, and calendar year effects using the analysis of variance and covariance (ANCOVA) models. We propose to include in the mean function the persistence terms in the conditional autoregressive range model for modelling the persistence of claim across development years. In the ANCOVA model, we adopt linear trends for the accident and calendar year effects and a quadratic trend for the development year effect. We investigate linear or log-transformed mean functions and four distributions, namely generalised beta type 2, generalised gamma, Weibull, and exponential extension, with positive support to enhance the model flexibility. The proposed models are implemented using the Bayesian user-friendly package Stan running in the R environment. Results show that the models with log-transformed mean function and persistence terms provide better model fits. Lastly, the best model is applied to forecast partial loss reserve and calendar year reserve for three years.
Assessing the relative influence of party unity on vote choice: evidence from a conjoint experiment
Authors: Lehrer Roni , Stöckle Pirmin , Juhl Sebastian 
Published: September 2022

AbstractObservational studies and anecdotal evidence suggest that party unity improves a party's electoral performance. Yet, due to a lack of experimental evidence, the causal standing of these findings remains unclear. Moreover, party unity manifests in various ways and we do not know how much different types of party unity affect the vote. Relying on a conjoint experiment implemented in a probability-based survey of the German population, our study unveils the distinct causal effect of different forms of party unity on vote choice. We further establish that appearing united can compensate for substantive policy distances between parties and voters. These findings have important implications for our understanding of how citizens vote and how intra-party politics affects the political representation of citizens in democracies.
Triacylglycerol fingerprint of sow milks during different lactation stages and from different breeds
Authors: Ren Cuirong , Jin Jun , Zhang Yanbing , Jin Qingzhe , Wang Xingguo 
Published: September 2022

AbstractSow milk fats not only provide energy but also essential nutrients for piglets. Thus, feeding strategies must be aligned with fat composition, especially triacylglycerols (TAGs) and their isomers. The triacylglycerol (TAG) profiles of sow milk fats from five typical breeds (Landrace × Large White, Landrace, Large White, Duroc, Pietrain) and two lactation stages (colostrum and milk) were systematically studied. A total of 45 major TAG species were identified using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The most abundant TAG was oleic acid-palmitic acid-linoleic acid (O-P-L) (13.92% and 12.03% in colostrum and milk, respectively), which was not significantly different in colostrum among all breeds. TAG composition of sow milk was affected mainly by the lactation stage rather than sow breed. Furthermore, TAG compositions of sow milk fats were similar to those of human milk fats, but significant differences were observed between commercial piglet formulas and sow milk. Therefore, the results will contribute to the optimization of piglet formulas to improve the growth and wellness of piglets, as well as potentially providing a basis for food usage as a new source of nutrients for human infants in future.
A Novel Cryogenic Adhesive Retaining Fluidity at Dry-Ice Temperature for Low-Temperature Scanning Electron Microscopy
Authors: Okada Satoshi , Chen Chong 
Published: September 2022

Abstract
Scanning electron microscopy operated at cryogenic temperature (cryo-SEM) is a powerful tool for investigating surface and cross-sectional nanostructures of water-containing samples. Typically, cryo-SEM samples are frozen just before observation in specific metal carriers. However, pre-frozen samples are also of interest, such as frozen food and freeze-stored animal samples. In such cases, sample mounting with a defined orientation is required, but there has been a lack of ideal conductive adhesives that can be used without increasing the sample temperature. Here, we developed a mixture of graphite oxide and 1,3-butanediol as an adhesive, capable of gluing samples at dry-ice temperature and is frozen below that temperature. Dispersion of graphite oxide increased the conductivity and reduced the charge-up contrast. Acquisition of energy-dispersive X-ray spectrum, cross-sectional ion milling, and high-resolution imaging were successfully achieved using the adhesive. We tested and confirmed the usefulness of this new adhesive by applying it to cryo-SEM surface imaging of diatomite, freeze-fractured cross-sectional imaging of chicken liver, and ion milling cross-sectional imaging of a deep-sea snail. The new adhesive is not only useful for food science and field-preserved biological samples but also potentially applicable to wider fields such as archaeological and biological samples preserved under permafrost.
LPS- and LTA-Induced Expression of TLR4, MyD88, and TNF-α in Lymph Nodes of the Akkaraman and Romanov Lambs
Authors: Alan Aydın , Alan Emel , Arslan Korhan , Daldaban Fadime , Aksel Esma Gamze , Çınar Mehmet Ulaş , Akyüz Bilal 
Published: September 2022

Abstract
Toll-like receptor (TLR)-mediated inflammatory processes play a critical role in the innate immune response during the initial interaction between the infecting microorganism and immune cells. This study aimed to investigate the possible microanatomical and histological differences in mandibular and bronchial lymph nodes in Akkaraman and Romanov lambs induced by lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and study the gene, protein, and immunoexpression levels of TLR4, myeloid differentiation factor 88 (MyD88), and tumor necrosis factor-α (TNF-α) that are involved in the immune system. Microanatomical examinations demonstrated more intense lymphocyte infiltration in the bronchial lymph nodes of Akkaraman lambs in the LPS and LTA groups compared to Romanov lambs. TLR4, MyD88, and TNF-α immunoreactivities were more intense in the experimental groups of both breeds. Expression levels of MyD88 and TNF-α genes in the bronchial lymph node of Akkaraman lambs were found to increase statistically significantly in the LTA group. TLR4 gene expression level in the mandibular lymph node was found to be statistically significantly higher in the LTA + LPS group. In conclusion, dynamic changes in the immune cell populations involved in response to antigens such as LTA and LPS in the lymph nodes of both breeds can be associated with the difference in the expression level of the TLR4/MyD88/TNF-α genes.
Neurobiological Changes in Posttraumatic Stress Disorder and Their Reversibility by Psychotherapy
Authors: Fischer Susanne , Schumacher Sarah , Daniels Judith 
Published: September 2022

Abstract. Background: Posttraumatic stress disorder (PTSD) is a debilitating illness associated with distressing symptoms and a high societal burden. Objective: To investigate the neurobiological underpinnings of PTSD to improve our understanding of this disorder and its treatment. Methods: This article reviews currently researched mechanisms that can explain the development of PTSD symptoms. It presents key findings on neural (i. e., brain functioning and brain structure), neuroendocrine (i. e., noradrenergic and hypothalamic-pituitary-adrenal axis activity), and related (epi)‌genetic changes in individuals with PTSD. Furthermore, it presents preliminary research examining the reversibility of these alterations during psychotherapeutic treatment. Results: PTSD is characterized by specific neurobiological alterations, with preliminary findings indicating that at least some of these may normalize during psychotherapy. Discussion: A multidimensional perspective on the development, maintenance, and treatment of PTSD has the potential to improve our understanding of the causal processes underlying the disorder and may ultimately inform the conception of novel treatments.
Stabilität und Prädiktion von Prüfungsangst bei Studierenden
Authors: Sommer Klara , Odenwald Michael , Fehm Lydia 
Published: September 2022

Zusammenfassung. Theoretischer Hintergrund: Obwohl Prüfungsangst ein häufiges Anliegen in psychologischen Beratungsstellen ist, wissen wir wenig über ihren zeitlichen Verlauf sowie über Risikofaktoren für hohe Prüfungsangst kurz vor den Prüfungen. Fragestellung: Diese Studie untersucht, ob sich die Intensität von Prüfungsangst während eines Semesters verändert und wie Personen mit hoher Belastung kurz vor der Prüfung früh identifiziert werden können. Methodik: Zu Beginn und kurz vor den Prüfungen des Wintersemesters 2014/15 wurden Prüfungsangst, Depressivität und Prokrastination bei 427 Studierenden (88.3 % Erstsemester; 68.4 % weiblich; Altersdurchschnitt 20.0 Jahre) erfasst. Ergebnisse: Die Analyse auf Einzelfallebene zeigte, dass sich die Prüfungsangst bei den meisten Studierenden nicht signifikant veränderte. Bei der Vorhersage der Prüfungsangst zum Semesterende stellten Prüfungsangst und Depressivität zu Semesterbeginn signifikante Prädiktoren dar. Diese wurden anhand von 80 % der Gesamtstichprobe ermittelt und an den anderen 20 % validiert. Schlussfolgerungen: Erhöhte Prüfungsangst und Depressivität zu Semesterbeginn können die frühe Identifikation von Studierenden mit bedeutsamer Prüfungsangst kurz vor Prüfungen ermöglichen.
Analyzing the Spatiotemporal Patterns of Forests Carbon Sink and Sources Between 2000 and 2019
Authors: Alaniz Alberto J. , Carvajal Mario A. , Marquet Pablo A. , Vergara Pablo M. , Meneses Luis , Moreira‐Arce Darío 
Published: September 2022

AbstractHere we present a global time‐series of global forest above ground biomass from 2000 to 2019, analyzing spatiotemporal patterns of carbon balance, accounting for losses and gains. We generated a global Above‐Ground Biomass (AGB) map for the year 2000 and assessed its correlation with different satellite products. We generated a multi‐year analysis of AGB changes at the pixel level was generated, estimating carbon (C) loss and gain. Finally, we estimated the C losses due to forest clearing and wildfires analyzing their trends across biomes and countries. Our results show that the global mean annual loss was 2.88 ± 0.33 PgC yr−1, while global mean C gain was 2.95 ± 0.43 PgC yr−1, resulting in a neutral to sink behavior of −0.06 ± 0.58 PgC yr−1. The mean annual C loss by forest clearing was 1.04 ± 0.03 PgC yr−1, with an increasing trend of +0.03 ± 0.01 PgC yr−1. Eight biomes and 54 countries showed a significant increasing trend of C loss by forest clearing. Wildfires C losses reached 0.351 ± 0.02 Pg C yr−1, representing the 33.71% of forest clearing C losses. Boreal forest presented the highest C losses from wildfires, while significant increasing trends were evidenced in five biomes. We also find increasing trends of wildfire C loss in 20 countries while decreasing trends were identified in 10 countries. Our findings highlight the importance of designing strong policies to halt deforestation as agreed in the recent COP26 and provide information to identify priority areas to start implementing these policies in the short term.
Radar Backscatter and Emissivity Models of Proposed Pyroclastic Density Current Deposits on Venus
Authors: Ganesh Indujaa , Carter Lynn M. , Henz Triana N. 
Published: September 2022

AbstractMagellan synthetic aperture radar observations of Venus revealed a small number of deposits in the highland regions that were suggested to have formed from pyroclastic density currents. Studying these deposits is useful for understanding the nature of pyroclastic activity and eruptive history on Venus. The proposed pyroclastic deposits occupy the uppermost unit in local stratigraphy and are found near exceptionally high reflectivity (∼0.6) units in the highlands. Their radar properties include high copolarized backscatter (∼−8 to −15 dB) and moderate emissivity values (∼0.70–0.88) in the 12.6 cm wavelength Magellan data acquired at incidence angles between ∼15° and 45°. We aim to characterize the structure of these deposits by modeling the observed backscatter and emissivity as a function of different physical and dielectric properties and shallow subsurface stratigraphy. Three different physical scenarios focusing on three different scattering mechanisms—surface scattering, subsurface scattering from buried dielectric horizons, and volume scattering from buried, distributed scatterers—are considered. By comparing the model results to Magellan observations, we narrow down likely pyroclastic deposit structures. We show that the deposits are likely analogous to dense, welded ignimbrites with high surface roughness. We also investigate other possible but less likely scenarios of a thin, low‐density, low‐loss mantling pyroclastic deposit on top of high reflectivity units and a thick, low‐density, low‐loss deposit with ∼5–10 volume % of scatterers of sub‐wavelength size. Future multiwavelength, multipolarization radar observations from VERITAS and EnVision may enable unambiguous characterization of these deposits.
Intact Polar brGDGTs in Arctic Lake Catchments: Implications for Lipid Sources and Paleoclimate Applications
Authors: Raberg Jonathan H. , Flores Edgart , Crump Sarah E. , de Wet Greg , Dildar Nadia , Miller Gifford H. , Geirsdóttir Áslaug , Sepúlveda Julio 
Published: September 2022

AbstractPaleotemperature histories derived from lake sediment archives provide valuable context for modern and future climate changes. Branched glycerol dialkyl glycerol tetraether (brGDGT) lipids are a valuable tool in such pursuits due to their empirical correlation with temperature and near ubiquity in nature. However, the relative contributions of terrestrial and lacustrine sources of brGDGTs to lake sediments is site‐dependent and difficult to constrain. Here, we explored the potential for intact brGDGTs—the complete lipids with polar head groups (HGs) still attached—to provide insight into the sources of brGDGTs on the landscape and their contributions to the sedimentary record in a set of Arctic lakes. We measured core and intact brGDGTs in soils, surface and downcore sediments, water filtrates, and sediment traps across five lake catchments in the Eastern Canadian Arctic, with an emphasis on Lake Qaupat (QPT), Baffin Island. Soils were dominated by brGDGTs with a monoglycosyl (1G) HG, while lacustrine samples contained more phosphohexose (PH) brGDGTs, providing evidence for in situ brGDGT production in both settings. Core‐ and PH‐brGDGT‐IIIa were more abundant in sediments than in the soils or water column, implying an additional post‐depositional source of brGDGTs. A hierarchical clustering analysis indicated that core brGDGTs in Lake QPT sediments were largely lacustrine in origin, while 1G‐brGDGTs were primarily soil‐derived. Additionally, we found evidence for preservation of intact brGDGTs—especially 1G‐brGDGTs—downcore on thousand‐year timespans, though in situ production deeper in the sediment column cannot be ruled out. Finally, we explored the possibility of reconstructing 1G‐brGDGT‐derived soil temperatures and core‐brGDGT‐derived lake temperatures in tandem from sedimentary archives.
Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling
Authors: Liu Chunlei , Li Peng , Ao Xiang , Lian Zhengnan , Liu Jie , Li Chenglong , Huang Minjun , Wang Liang , Zhang Zhongmin 
Published: September 2022

AbstractLigamentum flavum hypertrophy (LFH) is a major cause of lumbar spinal canal stenosis (LSCS). The pathomechanisms for LFH have not been fully elucidated. Isobaric tags for relative and absolute quantitation (iTRAQ) technology, proteomics assessments of human ligamentum flavum (LF), and successive assays were performed to explore the effect of clusterin (CLU) upregulation on LFH pathogenesis. LFH samples exhibited higher cell positive rates of the CLU, TGF-β1, α-SMA, ALK5 and p-SMAD3 proteins than non-LFH samples. Mechanical stress and TGF-β1 initiated CLU expression in LF cells. Notably, CLU inhibited the expression of mechanical stress-stimulated and TGF-β1-stimulated COL1A2 and α-SMA. Mechanistic studies showed that CLU inhibited mechanical stress-stimulated and TGF-β1-induced SMAD3 activities through suppression of the phosphorylation of SMAD3 and by inhibiting its nuclear translocation by competitively binding to ALK5. PRKD3 stabilized CLU protein by inhibiting lysosomal distribution and degradation of CLU. CLU attenuated mechanical stress-induced LFH in vivo. In summary, the findings showed that CLU attenuates mechanical stress-induced LFH by modulating the TGF-β1 pathways in vitro and in vivo. These findings imply that CLU is induced by mechanical stress and TGF-β1 and inhibits LF fibrotic responses via negative feedback regulation of the TGF-β1 pathway. These findings indicate that CLU is a potential treatment target for LFH.
Compartment-specific mutational landscape of clonal hematopoiesis
Authors: Hartmann Luise , Hecker Judith S. , Rothenberg-Thurley Maja , Rivière Jennifer , Jentzsch Madlen , Ksienzyk Bianka , Buck Michèle C. , van der Garde Mark , Fischer Luise , Winter Susann , Rauner Martina , Tsourdi Elena , Weidner Heike , Sockel Katja , Schneider Marie , Kubasch Anne S. , Nolde Martin , Hausmann Dominikus , Lützner Jörg , Goralski Szymon , Bassermann Florian , Spiekermann Karsten , Hofbauer Lorenz C. , Schwind Sebastian , Platzbecker Uwe , Götze Katharina S. , Metzeler Klaus H. 
Published: September 2022

AbstractClonal hematopoiesis (CH) is characterized by somatic mutations in blood cells of individuals without hematologic disease. While the mutational landscape of CH in peripheral blood (PB) has been well characterized, detailed analyses addressing its spatial and cellular distribution in the bone marrow (BM) compartment are sparse. We studied CH driver mutations in healthy individuals (n = 261) across different anatomical and cellular compartments. Variant allele frequencies were higher in BM than PB and positively correlated with the number of driver variants, yet remained stable during a median of 12 months of follow-up. In CH carriers undergoing simultaneous bilateral hip replacement, we detected ASXL1-mutant clones in one anatomical location but not the contralateral side, indicating intra-patient spatial heterogeneity. Analyses of lineage involvement in ASXL1-mutated CH showed enriched clonality in BM stem and myeloid progenitor cells, while lymphocytes were particularly involved in individuals carrying the c.1934dupG variant, indicating different ASXL1 mutations may have distinct lineage distribution patterns. Patients with overt myeloid malignancies showed higher mutation numbers and allele frequencies and a shifting mutation landscape, notably characterized by increasing prevalence of DNMT3A codon R882 variants. Collectively, our data provide novel insights into the genetics, evolution, and spatial and lineage-specific BM involvement of CH.
An epigenetic mechanism for over-consolidation of fear memories
Authors: Barchiesi Riccardo , Chanthongdee Kanat , Petrella Michele , Xu Li , Söderholm Simon , Domi Esi , Augier Gaelle , Coppola Andrea , Wiskerke Joost , Szczot Ilona , Domi Ana , Adermark Louise , Augier Eric , Cantù Claudio , Heilig Markus , Barbier Estelle 
Published: September 2022

AbstractExcessive fear is a hallmark of anxiety disorders, a major cause of disease burden worldwide. Substantial evidence supports a role of prefrontal cortex-amygdala circuits in the regulation of fear and anxiety, but the molecular mechanisms that regulate their activity remain poorly understood. Here, we show that downregulation of the histone methyltransferase PRDM2 in the dorsomedial prefrontal cortex enhances fear expression by modulating fear memory consolidation. We further show that Prdm2 knock-down (KD) in neurons that project from the dorsomedial prefrontal cortex to the basolateral amygdala (dmPFC-BLA) promotes increased fear expression. Prdm2 KD in the dmPFC-BLA circuit also resulted in increased expression of genes involved in synaptogenesis, suggesting that Prdm2 KD modulates consolidation of conditioned fear by modifying synaptic strength at dmPFC-BLA projection targets. Consistent with an enhanced synaptic efficacy, we found that dmPFC Prdm2 KD increased glutamatergic release probability in the BLA and increased the activity of BLA neurons in response to fear-associated cues. Together, our findings provide a new molecular mechanism for excessive fear responses, wherein PRDM2 modulates the dmPFC -BLA circuit through specific transcriptomic changes.
VAL1 acts as an assembly platform co-ordinating co-transcriptional repression and chromatin regulation at Arabidopsis FLC
Authors: Mikulski Pawel , Wolff Philip , Lu Tiancong , Nielsen Mathias , Echevarria Elsa Franco , Zhu Danling , Questa Julia I. , Saalbach Gerhard , Martins Carlo , Dean Caroline 
Published: September 2022

AbstractPolycomb (PcG) silencing is crucial for development, but how targets are specified remains incompletely understood. The cold-induced Polycomb Repressive Complex 2 (PRC2) silencing of Arabidopsis thaliana FLOWERING LOCUS C (FLC) provides an excellent system to elucidate PcG regulation. Association of the DNA binding protein VAL1 to FLC PcG nucleation regionis an important step. VAL1 co-immunoprecipitates APOPTOSIS AND SPLICING ASSOCIATED PROTEIN (ASAP) complex and PRC1. Here, we show that ASAP and PRC1 are necessary for co-transcriptional repression and chromatin regulation at FLC. ASAP mutants affect FLC transcription in warm conditions, but the rate of FLC silencing in the cold is unaffected. PRC1-mediated H2Aub accumulation increases at the FLC nucleation region during cold, but unlike the PRC2-delivered H3K27me3, does not spread across the locus. H2Aub thus involved in the transition to epigenetic silencing at FLC, facilitating H3K27me3 accumulation and long-term epigenetic memory. Overall, our work highlights the importance of VAL1 as an assembly platform co-ordinating activities necessary for epigenetic silencing at FLC.