bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipopolysaccharide-Induced Bone Loss in Rodent Models: A Systematic Review and Meta-Analysis
Authors: Bott Kirsten N. , Feldman Evelyn , de Souza Russell J. , Comelli Elena M. , Klentrou Panagiota , Peters Sandra J. , Ward Wendy E. 
Published: November 2022

ABSTRACT
Osteoporosis has traditionally been characterized by underlying endocrine mechanisms, though evidence indicates a role of inflammation in its pathophysiology. Lipopolysaccharide (LPS), a component of gram-negative bacteria that reside in the intestines, can be released into circulation and stimulate the immune system, upregulating bone resorption. Exogenous LPS is used in rodent models to study the effect of systemic inflammation on bone, and to date a variety of different doses, routes, and durations of LPS administration have been used. The study objective was to determine whether systemic administration of LPS induced inflammatory bone loss in rodent models. A systematic search of Medline and four other databases resulted in a total of 110 studies that met the inclusion criteria. Pooled standardized mean differences (SMDs) and corresponding 95% confidence intervals (CI) with a random-effects meta-analyses were used for bone volume fraction (BV/TV) and volumetric bone mineral density (vBMD). Heterogeneity was quantified using the I2 statistic. Shorter-term (&lt;2 weeks) and longer-term (&gt;2 weeks) LPS interventions were analyzed separately because of intractable study design differences. BV/TV was significantly reduced in both shorter-term (SMD = −3.79%, 95% CI [−4.20, −3.38], I2 62%; p &lt; 0.01) and longer-term (SMD = −1.50%, 95% CI [−2.00, −1.00], I2 78%; p &lt; 0.01) studies. vBMD was also reduced in both shorter-term (SMD = −3.11%, 95% CI [−3.78, −2.44]; I2 72%; p &lt; 0.01) and longer-term (SMD = −3.49%, 95% CI [−4.94, −2.04], I2 82%; p &lt; 0.01) studies. In both groups, regardless of duration, LPS negatively impacted trabecular bone structure but not cortical bone structure, and an upregulation in bone resorption demonstrated by bone cell staining and serum biomarkers was reported. This suggests systemically delivered exogenous LPS in rodents is a viable model for studying inflammatory bone loss, particularly in trabecular bone. © 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
Association of polymorphisms in the IL‐10 promoter region with Crohn's disease
Authors: Sun Anna , Li Wei , Shang Shiqiang 
Published: November 2022

AbstractBackgroundIL‐10 is thought to play an important role in preventing inflammatory bowel disease (IBD), although its efficacy is limited in IBD inflammation treatment. The purpose of this study is to investigate the association between SNP polymorphism in the promoter region of the IL‐10 gene and Crohn's disease (CD).MethodsIn 86 children with CD disease and 142 healthy controls, polymorphisms of three SNPs (rs3790622, rs1800872, and rs1800896) in the IL‐10 promoter region were successfully identified. The risk alleles, genotypes, and haplotypes were also analyzed in the CD patient group and the control group. 2 × 2 chi‐square test was used to identify whether there is a statistically significant association between CD risk and SNP polymorphisms.ResultsAccording to the chi‐square test results, only the polymorphism of rs1800872 was associated with pediatric CD. T allele in rs1800872 showed a high risk for pediatric CD (Pearson χ2 p = 0.030). TT genotype of rs1800872 was associated with a higher risk of CD in the pediatric population (OR 1.986, 95% CI 1.146–3.442, p = 0.020, TT vs. TG + GG). Finally, a risk haplotype GTT (rs3790622‐rs1800872‐rs1800896) in IL‐10 was found (OR 1.570, 95% CI 1.054–2.341, p = 0.028).ConclusionsOur data suggested that T allele, TT genotype, and haplotype GTT in rs1800872 were associated with the susceptibility to pediatric CD in China.
Intercellular communication among liver cells in the perisinusoidal space of the injured liver: Pathophysiology and therapeutic directions
Authors: Ezhilarasan Devaraj , Najimi Mustapha 
Published: November 2022

AbstractHepatic stellate cells (HSCs) in the perisinusoidal space are surrounded by hepatocytes, liver sinusoidal endothelial cells, Kupffer cells, and other resident immune cells. In the normal liver, HSCs communicate with these cells to maintain normal liver functions. However, after chronic liver injury, injured hepatocytes release several proinflammatory mediators, reactive oxygen species, and damage‐associated molecular patterns into the perisinusoidal space. Consequently, such alteration activates quiescent HSCs to acquire a myofibroblast‐like phenotype and express high amounts of transforming growth factor‐β1, angiopoietins, vascular endothelial growth factors, interleukins 6 and 8, fibril forming collagens, laminin, and E‐cadherin. These phenotypic and functional transdifferentiation lead to hepatic fibrosis with a typical abnormal extracellular matrix synthesis and disorganization of the perisinusoidal space of the injured liver. Those changes provide a favorable environment that regulates tumor cell proliferation, migration, adhesion, and survival in the perisinusoidal space. Such tumor cells by releasing transforming growth factor‐β1 and other cytokines, will, in turn, activate and deeply interact with HSCs via a bidirectional loop. Furthermore, hepatocellular carcinoma‐derived mediators convert HSCs and macrophages into protumorigenic cell populations. Thus, the perisinusoidal space serves as a critical hub for activating HSCs and their interactions with other cell types, which cause a variety of liver diseases such as hepatic inflammation, fibrosis, cirrhosis, and their complications, such as portal hypertension and hepatocellular carcinoma. Therefore, targeting the crosstalk between activated HSCs and tumor cells/immune cells in the tumor microenvironment may also support a promising therapeutic strategy.
Capsaicin alleviates cisplatin‐induced muscle loss and atrophy in vitro and in vivo
Authors: Huang Ko‐Chieh , Chiang Yi‐Fen , Huang Tsui‐Chin , Chen Hsin‐Yuan , Lin Po‐Han , Ali Mohamed , Hsia Shih‐Min 
Published: November 2022

AbstractBackgroundCisplatin (CP) is a widely used chemotherapeutic drug with subsequent adverse effects on different organs and tissues including skeletal muscle loss and atrophy as the most common clinical symptoms. The molecular mechanism of cisplatin‐induced muscle atrophy is not clearly understood. However, recent significant advances indicate that it is related to an imbalance in both the protein status and apoptosis. Capsaicin (CAP) is one of the major ingredients in chilli peppers. It is a valuable pharmacological agent with several therapeutic applications in controlling pain and inflammation with particular therapeutic potential in muscle atrophy. However, the mechanisms underlying its protective effects against cisplatin‐induced muscle loss and atrophy remain largely unknown. This study aims to investigate capsaicin's beneficial effects on cisplatin‐induced muscle loss and atrophy in vitro and in vivo.MethodsThe anti‐muscle‐atrophic effect of capsaicin on cisplatin‐induced muscle loss was investigated using in vivo and in vitro studies. By using the pretreatment model, pretreated capsaicin for 24 h and treated with cisplatin for 48 h, we utilized a C2C12 myotube formation model where cell viability analysis, immunofluorescence, and protein expression were measured to investigate the effect of capsaicin in hampering cisplatin‐induced muscle atrophy. C57BL/6 mice were administered capsaicin (10, 40 mg/kg BW) as a pretreatment for 5 weeks and cisplatin (3 mg/kg BW) for seven consecutively days to assess muscle atrophy in an animal model for protein and oxidative stress examination, and the grip strength was tested to evaluate the muscle strength.ResultsOur study results indicated that cisplatin caused lower cell viability and showed a subset of hallmark signs typically recognized during atrophy, including severe reduction in the myotube diameter, repression of Akt, and mTOR protein expression. However, pretreatment with capsaicin could ameliorate cisplatin‐induced muscle atrophy by up‐regulating the protein synthesis in skeletal muscle as well as down‐regulating the markers of protein degradation. Additionally, capsaicin was able to downregulate the protein expression of apoptosis‐related markers, activated TRPV1 and autophagy progress modulation and the recovery of lysosome function. In vivo, capsaicin could relieve oxidative stress and cytokine secretion while modulating autophagy‐related lysosome fusion, improving grip strength, and alleviating cisplatin‐induced body weight loss and gastrocnemius atrophy.ConclusionsThese findings suggest that capsaicin can restore cisplatin‐induced imbalance between protein synthesis and protein degradation pathways and it may have protective effects against cisplatin‐induced muscle atrophy.
GABAergic neurons maturation is regulated by a delicate network
Authors: Gao Mingxing , Wang Kaizhong , Zhao Hui 
Published: November 2022

AbstractGamma‐aminobutyric acid‐expressing (GABAergic) neurons are implicated in a variety of neuropsychiatric disorders, such as epilepsy, anxiety, autism, and other pathological processes, including cerebral ischemia injury and drug addiction. Therefore, GABAergic neuronal processes warrant further research. The development of GABAergic neurons is a tightly controlled process involving the activity of multiple transcription and growth factors. Here, we focus on the gene expression pathways and the molecular modulatory networks that are engaged during the development of GABAergic neurons with the goal of exploring regulatory mechanisms that influence GABAergic neuron fate (i.e., maturation). Overall, we hope to provide a basis for clarifying the pathogenesis of neurodegenerative disorders.
Impact of tumor size on the difficulty of laparoscopic left lateral sectionectomies
Authors: Aizza Giada , Russolillo Nadia , Ferrero Alessandro , Syn Nicholas L. , Cipriani Federica , Aghayan Davit , Marino Marco V. , Memeo Riccardo , Mazzaferro Vincenzo , Chiow Adrian K. H. , Sucandy Iswanto , Ivanecz Arpad , Vivarelli Marco , Di Benedetto Fabrizio , Choi Sung‐Hoon , Lee Jae Hoon , Park James O. , Gastaca Mikel , Fondevila Constantino , Efanov Mikhail , Rotellar Fernando , Choi Gi‐Hong , Robles‐Campos Ricardo , Wang Xiaoying , Sutcliffe Robert P. , Pratschke Johann , Tang Chung Ngai , Chong Charing C. , D'Hondt Mathieu , Yong Chee Chien , Ruzzenente Andrea , Herman Paolo , Kingham T. Peter , Scatton Olivier , Liu Rong , Levi Sandri Giovanni Battista , Soubrane Olivier , Mejia Alejandro , Lopez‐Ben Santiago , Monden Kazateru , Wakabayashi Go , Cherqui Daniel , Troisi Roberto I. , Yin Mengqiu , Giuliante Felice , Geller David , Sugioka Atsushi , Edwin Bjorn , Cheung Tan‐To , Long Tran Cong Duy , Hilal Mohammad Abu , Fuks David , Chen Kuo‐Hsin , Aldrighetti Luca , Han Ho‐Seong , Goh Brian K. P. ,   International Robotic and Laparoscopic Liver Resection study group investigators
Published: November 2022

AbstractBackgroundTumor size (TS) represents a critical parameter in the risk assessment of laparoscopic liver resections (LLR). Moreover, TS has been rarely related to the extent of liver resection. The aim of this study was to study the relationship between tumor size and difficulty of laparoscopic left lateral sectionectomy (L‐LLS).MethodsThe impact of TS cutoffs was investigated by stratifying tumor size at each 10 mm‐interval. The optimal cutoffs were chosen taking into consideration the number of endpoints which show a statistically significant split around the cut‐points of interest and the magnitude of relative risk after correction for multiple risk factors.ResultsA total of 1910 L‐LLS were included. Overall, open conversion and intraoperative blood transfusion were 3.1 and 3.3%, respectively. The major morbidity rate was 2.7% and 90‐days mortality 0.6%. Three optimal TS cutoffs were identified: 40‐, 70‐, and 100‐mm. All the selected cutoffs showed a significant discriminative power for the prediction of open conversion, operative time, blood transfusion and need of Pringle maneuver. Moreover, 70‐ and 100‐mm cutoffs were both discriminative for estimated blood loss and major complications. A stepwise increase in rates of open conversion rate (Z = 3.90, P < .001), operative time (Z = 3.84, P < .001), blood loss (Z = 6.50, P < .001), intraoperative blood transfusion rate (Z = 5.15, P < .001), Pringle maneuver use (Z = 6.48, P < .001), major morbidity(Z = 2.17, P = .030) and 30‐days readmission (Z = 1.99, P = .047) was registered as the size increased.ConclusionL‐LLS for tumors of increasing size was associated with poorer intraoperative and early postoperative outcomes suggesting increasing difficulty of the procedure. We determined three optimal TS cutoffs (40‐, 70‐ and 100‐mm) to accurately stratify surgical difficulty after L‐LLS.
A vignette study of novices' interviewing skills of asylum seekers
Authors: Kask Kristjan , Groznov Nikita , van Veldhuizen Tanja 
Published: November 2022

AbstractThe aim of the current research was to replicate Van Veldhuizen, Horselenberg, Landstrom, et al.’s (2017) vignette study among novice Estonian police cadets to map their interviewing skills. Sixty‐one police cadets from the Estonian Academy of Security Sciences were asked to respond to one of four vignettes that contained fictitious asylum narratives. Two of the vignettes contained no evidence for the origin of the asylum seeker, and the other two contained no evidence for the claim of persecution. The cadets were asked to formulate five questions that would help them to assess the credibility of the applicant's claim. We coded the style, type, and content of the questions. Our analyses showed that, in line with best practice, the cadets mainly formulated open questions in an information‐gathering style. A thematic analysis revealed that when a claim about origin was assessed, cadets typically formulated questions about life in the country of origin, identity documents, and the flight to Europe. When assessing a persecution claim, in contrast, they mostly formulated case‐specific questions.
Infection phase‐dependent dynamics of the viral and host N6‐methyladenosine epitranscriptome in the lifecycle of an oncogenic virus in vivo
Authors: Zhuang Guoqing , Zhao Xuyang , Jin Jiaxin , Zhu Xiaojing , Wang Rui , Zhai Yunyun , Lu Wenlong , Liao Yifei , Teng Man , Yao Yongxiu , Nair Venugopal , Yao Wen , Sun Aijun , Luo Jun , Zhang Gaiping 
Published: November 2022

AbstractDynamic alteration of the epitranscriptome exerts regulatory effects on the lifecycle of oncogenic viruses in vitro. However, little is known about these effects in vivo because of the general lack of suitable animal infection models of these viruses. Using a model of rapid‐onset Marek's disease lymphoma in chickens, we investigated changes in viral and host messenger RNA (mRNA) N6‐methyladenosine (m6A) modification during Marek's disease virus (MDV) infection in vivo. We found that the expression of major epitranscriptomic proteins varies among viral infection phases, reprogramming both the viral and the host epitranscriptomes. Specifically, the methyltransferase‐like 3 (METTL3)/14 complex was suppressed during the lytic and reactivation phases of the MDV lifecycle, whereas its expression was increased during the latent phase and in MDV‐induced tumors. METTL3/14 overexpression inhibits, whereas METTL3/14 knockdown enhances, MDV gene expression and replication. These findings reveal the dynamic features of the mRNA m6A modification program during viral replication in vivo, especially in relation to key pathways involved in tumorigenesis.
The effect of SARS‐CoV‐2 infection on cardiac function in post‐COVID‐19 survivors: A systematic review and meta‐analysis
Authors: Rahmati Masoud , Koyanagi Ai , Banitalebi Ebrahim , Yon Dong Keon , Lee Seung Won , Il Shin Jae , Smith Lee 
Published: November 2022

AbstractThe longitudinal trajectories of cardiac structure and function following SARS‐CoV‐2 infection are unclear. Therefore, this meta‐analysis aims to elucidate the effect of SARS‐CoV‐2 infection on cardiac function in coronavirus disease 2019 (COVID‐19) survivors after recovery.PubMed/MEDLINE, CENTRAL, and EMBASE were systematically searched for articles published up to 1st August 2022. A systematic review and meta‐analysis were performed to calculate the pooled effects size and 95% confidence interval of each outcome.A total of 21 studies including 2394 individuals (1436 post‐COVID‐19 cases and 958 controls) were included in the present meta‐analysis. The pooled analyses compared with control groups showed a significant association between post‐COVID‐19 and reduced left ventricular ejection fraction (LV EF), LV end‐diastolic volume (LV EDV), LV stroke volume (LV SV), mitral annular plane systolic excursion (MAPSE), global longitudinal strain, right ventricular EF (RV EF), RV EDV, RV ESV, RV SV, tricuspid annular plane systolic excursion, and increased LV mass. Subgroup analysis based on the severity of COVID‐19 in the acute phase and subsequent chronic outcomes revealed that LV EF, MAPSE, RV EF, and RV ESV only decreased in studies including patients with a history of intensive care unit admission.Cardiac impairment after SARS‐CoV‐2 infection persisted in recovered COVID‐19 patients even after 1 year. Future studies are warranted to determine the biological mechanisms underlying the long‐term cardiovascular consequences of COVID‐19.
Raman spectroscopy for the quantitative analysis of Lornoxicam in solid dosage forms
Authors: Aymen Sammia , Nawaz Haq , Majeed Muhammad Irfan , Rashid Nosheen , Ehsan Usama , Shahzad Rida , Ali Muhammad Zeeshan , Rimsha Gull , Fatima Rida , Meraj Lubna , Sadaf Nimra , Ali Zain 
Published: November 2022

AbstractIn this study, Raman spectroscopy is used to characterize different formulations of active pharmaceutical ingredient (API) of Lornoxicam, a nonsteroidal anti‐inflammatory drug for qualitative and quantitative analyses. The Raman spectral characteristic peaks associated with API and excipients are identified, which increase or decrease as their concentrations change in different formulations. The spectral response was analyzed qualitatively and quantitatively by using chemometrics tools such as principal component analysis (PCA) model and partial least squares regression (PLSR) analysis model, respectively. The characteristic Raman spectral features associated with the different formulations of API of Lornoxicam were used to differentiate different groups of Raman spectral data using PCA. By using PLSR model, root mean square error of cross validation (RMSECV) was found to be 0.76 mg during the development of prediction model and according to the (R2) value, the goodness of the model was found to be 0.99. Furthermore, the concentration of API in the blind sample was measured. In comparison to the 15/35 mg (w/w), this concentration was projected to be 14.13/35 mg (w/w). Raman spectroscopy along with PCA and PLSR analyses is a useful tool for determining the concentration of Lornoxicam in pharmaceutical samples, both qualitatively and quantitatively.
Eczema herpeticum and impetigo herpetiformis, is there a link?
Authors: Cassalia Fortunato , Tartaglia Jacopo , Zardo Davide , Caroppo Francesca , Belloni Fortina Anna 
Published: November 2022

AbstractImpetigo herpetiformis (IH) is a rare variant of pustular psoriasis with typical onset during the third trimester of pregnancy; it is characterized by erythematous plaques lined with sterile pustules and sometimes there may be systemic involvement. On the other hand, eczema herpeticum (EH) is defined as a disseminated skin infection by herpes simplex virus type 1, which mainly affects patients characterized by an impaired integrity of the skin barrier, mostly atopic dermatitis. We report the case of a 30‐year‐old woman, primigravida at 28 + 3 weeks of gestation, who after asymptomatic severe acute respiratory syndrome coronavirus 2 infection for about 7 days presented to the emergency room with a burning and itching rash on the trunk and limbs. She reported a long‐standing history of atopic dermatitis and recurrent herpetic eruptions. The diagnosis of EH was made and systemic therapy was set with excellent results. A few days later, the patient developed a pustular rash in the areas previously affected by EH, with histologic features of IH. A relation between EH and IH is thus suggested.
Influencers' “organic” persuasion through electronic word of mouth: A case of sincerity over brains and beauty
Authors: Filieri Raffaele , Acikgoz Fulya , Li Chunyu , Alguezaui Salma 
Published: November 2022

AbstractDespite the growing importance of influencers' word‐of‐mouth through audiovisual content, little is known about its effect on consumers' brand evaluation, purchase intentions, and decisions. Drawing on Ohanian's source credibility framework, we conducted two studies across different influencers, product categories, and respondents' gender and tested the hypotheses using covariance‐based and partial least square structural equation modeling. Study 1 focuses on a mega‐influencer of cosmetic and beauty brands and predominantly involves female respondents. The findings show that the influencer's attractiveness affects perceptions about source expertise and source trustworthiness but not brand attitude, while source expertise predicts source trustworthiness, and both mediate the effect of source attractiveness on brand attitude. In contrast, brand attitude predicts purchase intention and mediates the impact of source credibility dimensions. Study 2 focuses on various influencers of hedonic products (lifestyle, fashion, and beauty). The results confirm the influence of source attractiveness and expertise on source trustworthiness, which ultimately predicts consumer purchase decisions. This study reveals the interdependencies between different source constructs, contributing to source credibility theory. Furthermore, we show that the effect of source dimensions that are relevant in the celebrity endorsement literature, such as source attractiveness, do not directly influence consumers' intentions and decisions in the context of influencers' electronic word of mouth. Finally, the two studies confirm that only influencers perceived as honest and sincere can influence consumers' purchase decisions.
Specificity of the decision‐making mechanism in public corporations
Authors: Yang Xiaoke , Wu Lidong 
Published: November 2022

AbstractThe purpose of the study is to determine the system of differences in the decision‐making algorithms between public corporations and private ones for similar tasks. It was determined that situational and behavioral factors influence the decision‐making process in corporations. Based on the interpretation of the results of the survey of heads of private and public corporations, models for structuring decisions in public and private corporations have been built. The analysis of the constructed models made it possible to identify and formulate a system of differences in the decision‐making mechanism and factors influencing decision making in public corporations.