bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo evaluation of a novel 18F-labeled PET radioligand for translocator protein 18kDa (TSPO) in monkey brain
Authors: Yan Xuefeng , Siméon Fabrice G. , Liow Jeih-San , Morse Cheryl L. , Santamaria Jose A. Montero , Jenkins Madeline , Manly Lester S. , Buskirk Maia Van , Zoghbi Sami S. , Pike Victor W. , Innis Robert B. , Zanotti-Fregonara Paolo 
Published: October 2022

Abstract
Purpose: 18F-SF51 was previously found to have high binding affinity and selectivity for 18kDa translocator protein (TSPO) in mouse brain. This study sought to further evaluate the suitability of 18F-SF51 for absolute quantification of TSPO in monkey brain.
Methods: Positron emission tomography (PET) imaging was performed in monkey brain (n=3) at baseline and after pre-blockade with the TSPO ligands PK11195 and PBR28. TSPO binding was calculated as total distribution volume corrected for free parent fraction in plasma (VT/fP) using a two-tissue compartment model. Receptor occupancy and nondisplaceable uptake were determined via Lassen plot. Binding potential (BPND) was calculated as the ratio of specific binding to nondisplaceable uptake. Time stability of VT was used as an indirect probe to detect radiometabolite accumulation in the brain. In vivo and ex vivo experiments were performed in mice to determine the distribution of the radioligand.
Results: After 18F-SF51 injection, the concentration of brain radioactivity peaked at 2.0 standardized uptake value (SUV) at ~10 minutes and declined to 30% of the peak at 180 minutes. VT/fP at baseline was generally high (203±15 mL· cm-3) and decreased by ~90% after blockade with PK11195. BPND of the whole brain was 7.6 ± 4.3. VT values reached levels similar to terminal 180-minute values by 70 minutes and remained relatively stable thereafter with excellent identifiability (standard errors < 5%), suggesting that no significant radiometabolites accumulated in the brain. Ex vivo experiments in mouse brain showed that 96% of radioactivity was parent. No significant uptake was observed in the skull, suggesting a lack of defluorination in vivo.
Conclusion: The results demonstrate that 18F-SF51 is an excellent radioligand with a good ratio of specific to nondisplaceable uptake as well as good time stability of total receptor binding. Collectively, the results suggest that 18F-SF51 warrants further evaluation in humans.
Robust optimization of consistency in filling of rib-grooves for titanium alloy multi-rib eigenstructure
Authors: Ding Tong , Wei Ke , Yang Chao , Tang HaiBing 
Published: October 2022

Abstract
Isothermal forging is an effective method for forming and manufacturing large-scale titanium alloy components with multi-rib. However, successive filling of the rib-grooves and reverse flow of the material are prone to occur during the forming process, which makes those rib-grooves are difficult to be filled then resulting in disturbed material flow and excessive die loading. The variability of billet sizes and fluctuation of uncertain parameters during the forging process have the great impacts on the forming results and stability. To this end, the eigenstructure with multi-rib from large titanium alloy rib-web components was extracted, and the combined method of finite element simulation and physical simulation experiment was used based on isothermal forging technique. Firstly, the finite element model for the eigenstructure under isothermal forging is established, then the behavior of the material flow and rib-grooves filling in the die cavity are analyzed. Secondly, the variation pattern and fluctuation range of rib-grooves filling are investigated by considering the deterministic factors of billet sizes, as well as the uncertainties of die draft angle, forming temperature, forming speed, billet manufacturing deviation and die manufacturing deviation. Subsequently, the significate deterministic factors and uncertainty factors are screened out and the correlation between the mean value as well as the variance of the filling consistency of the rib-grooves and the deterministic factors i.e. billet sizes are established by the dual response surface method. Then, a robust optimization model is constructed and solved. Finally, the reliability of the robust optimization solution is compared and verified, to obtain the ideal and stable fully-filling of the rib-grooves by adjusting and regulating the deterministic factors to weaken the interference of the uncertainties and achieve the simultaneous filling of the rib-grooves.
Insufficient HtrA2 causes meiotic defects by inducing cytoskeletal disorganization in aging germinal vesicle oocytes
Authors: Gao Min , Qiu Yanling , Cao Tianqi , Li Dungao , Wang Jingwen , Jiao Yiren , Chen Zhiyun , Huang Junjiu 
Published: October 2022

Abstract

Background High-temperature requirement protease A2 (HtrA2/Omi) is a mitochondrial chaperone highly conserved from bacteria to humans. It plays an important role in mitochondrial homeostasis and apoptosis. In this study, we investigated the role of HtrA2 in mouse oocyte maturation. Methods The role of HtrA2 in mouse oocyte maturation was investigated by employing knockdown (KD) or overexpression (OE) of HtrA2 in young or old GV oocytes. We employed immunoblotting, immunostaining, fluorescent intensity quantification to test the HtrA2 knockdown on the GV oocyte maturation progression, spindle assembly checkpoint, mitochondrial distribution, spindle assembly, chromosome alignment, actin polymerization, DNA damage and chromosome numbers, the level of acetylated tubulin. Results We observed a significant reduction in HtrA2 protein levels in aging germinal vesicle (GV) oocytes. Young oocytes with low levels of HtrA2 due to siRNA knockdown were unable to complete meiosis and were partially blocked at metaphase I (MI). They also displayed significantly more BubR1 on kinetochores, indicating that the spindle assembly checkpoint was triggered at MI. Extrusion of the first polar body (Pb1) was significantly less frequent and oocytes with large polar bodies were observed when HtrA2 was depleted. In addition, HtrA2 knockdown induced meiotic spindle/chromosome disorganization, leading to aneuploidy at metaphase II (MII), possibly due to the elevated level of acetylated tubulin. Importantly, overexpression of HtrA2 partially rescued spindle/chromosome disorganization and reduced the rate of aneuploidy in aging oocytes. Conclusions Collectively, our data suggest that HtrA2 is a key regulator of oocyte maturation, and its deficiency with age appears to contribute to reproduction failure in females.
Improved adsorption of fluorine on three typical saline-sodic soils by increasing functional groups with Al 2 (SO 4 ) 3 incorporation
Authors: Zang Jinyu , Wang Nan , Huang Ning , Wang Hongbin , Sui Biao , Zhao Chenyu , Zhao Xingmin , Liu Jinhua 
Published: October 2022

Abstract

Fluorine pollution in saline-sodic soils has become an important environmental problem, but little is known about the interaction between Al2(SO4)3 and fluorine when Al2(SO4)3 is used to improve saline-sodic soils. In this study, batch adsorption experiments were conducted to investigate the impacts of different saline-sodic soils with Al2(SO4)3 on fluorine adsorption, and the mechanisms of adsorption were characterized by SEM, BET, FTIR, and XPS analysis. The results showed that fluorine adsorption decreased with increasing soil salinity, and the addition of Al2(SO4)3 increased the adsorption of fluorine in soils. Among the three saline-sodic soils, Al2(SO4)3 contributed greatly to fluorine adsorption by mildly saline-sodic soils, with a range of 40.84%-69.70%. Fluorine adsorption isotherms could be properly described by the Langmuir and Freundlich models. At higher fluorine equilibrium concentrations, the adsorption amount of fluorine was greater for soils with Al→F. Both the soil porosity and the number of aggregates of saline-sodic soils were increased after adding Al2(SO4)3 for adsorption. Functional groups such as Si-O-Si, H-O-H, C-H, -OH, and Al-O-H were involved in the reaction process. Fluorine adsorption on saline-sodic soils refers to the ligand exchange and electrostatic adsorption, and F− replaces -OH on the soil surface. This work may provide a theoretical reference for the changes in fluorine chemical behavior when Al2(SO4)3 ameliorates saline-sodic soils.
Emotion recognition from gait using multi-scale directed adaptive spatio-temporal graph convolutional network
Authors: Zhang Jiabo , Gao Jie , Huang Zhongyu , Xu Guanghu 
Published: October 2022

Abstract
Graph convolutional network have been widely used for walking emotion recognition. However, the dependencies in the multi-scale,long-distance data and neighbor nodes can affect the efficiency of emotion feature extraction, and further impact the emotion identification accuracy. To this end, we propose i) a multi-scale spatio-temporal information directed aggregation method based on directed graphs, which considers not only the correlation between spatial and temporal features at the same time, but also the directionality of the temporal dimension; ii)a multi-scale directed adaptive spatio-temporal graph convolutional network (MSDAST-GCN), where the features of each spatio-temporal graph node are extracted according to the time span, aggregation scale and size. The MSDAST-GCN extracts motion data from a video, reasons about human emotions and classifies the emotions accurately into four categories: happy, sad, angry and normal. The MSDAST-GCN outperforms previous state-of-the-art methods on the latest dataset E-Gait, shows a 3.5% accuracy improvement. Specifically, the MSDAST-GCN achieves 1.4%, 0.4%, 2.9% and 7.5% accuracy enhancements on the happy, sad, angry and normal categories, respectively.
A Review of the Essential Visual Skills Required for Rugby: Beyond 20-20 Optometry
Authors: Millard Lourens , Breukelman Gerrit Jan , Burger Teriza , Nortje Joël , Schülz Jessica 
Published: October 2022

Abstract
Background: Vision is one of the most important qualities required of athletes. It provides players with an ability to perform sport-related drills, as well as applying decision-making skills. In order to accurately measure the visual ability of athletes, it is important to first identify the variety of visual skills involved in the sport, in this case rugby.
Objectives: The objectives of the review novelty are to identify the most important visual skills for rugby. To create a referencing point for future studies to be able to add additional visual skills essential to rugby players.
Methods: An electronic search was conducted to assess the scientific literature relating to the visual skills required for rugby. Only peer-reviewed articles written in English were included in the search. Data on the relationship with match performance, the problem definition or purpose of the study, and the inclusion of a theoretical definition of tactical behavior were extracted.
Discussion: The most important visual skills in rugby are: Visual acuity, Eye tracking, Eye focusing, Depth perception, Hand-eye coordination, Fixation, Fusion flexibility, Visual adjustability and Colour flexibility. Rugby players must pay attention to their teammates' positions, understand the opponent's actions and tactics, handle the ball, analyze the current situation, and predict what will happen.
Changes in HER3 expression profiles between primary and recurrent gynecological cancers
Authors: Kojima Yuki , Sudo Kazuki , Yoshida Hiroshi , Yazaki Shu , Tokura Momoko , Mizoguchi Chiharu , Okuma Hitomi S , Kita Shosuke , Yamamoto Kasumi , Nishikawa Tadaaki , Noguchi Emi , Shimoi Tatsunori , Tanase Yasuhito , Uno Masaya , Ishikawa Mitsuya , Kato Tomoyasu , Koyama Kumiko , Kobayashi Maki , Kakegawa Tomoya , Fujiwara Yasuhiro , Yonemori Kan 
Published: October 2022

Abstract
Background
Human epidermal growth factor receptor-3 (HER3) is a member of the epidermal growth factor receptor family of receptor tyrosine kinases, and its overexpression is associated with inferior prognosis in several cancers. However, it is unclear whether HER3 expression status changes in tumor tissue at recurrence. Therefore, this study aimed to evaluate the changes in HER3 expression between primary and recurrent status in gynecological cancers.
Methods
This retrospective study used matched-pair tissues of gynecological cancer patients at initial diagnosis and at recurrence. Immunohistochemical (IHC) scores of 3 + or 2 + were termed “HER3-high”, while IHC scores of 1 + or 0 were designated as “HER3-low/zero”.
Results
A total of 86 patients (40 with ovarian cancers, 32 with endometrial cancers, and 14 with cervical cancers) were included in this study. In ovarian cancer, 67.5% and 80.0% of the patients received a HER3-high at initial and recurrent diagnosis, respectively. The H-score was significantly increased at recurrence (p = 0.004). The proportion of HER3-high endometrial cancer patients increased from 46.9% at initial diagnosis to 68.8% at recurrence, and the H-score tended to increase at recurrence (p = 0.08). The fraction of HER3-high-rated cervical cancer patients remained unchanged at 85.7% both at initial and recurrent diagnosis. The discordance rate of HER3 expression detection in initial and recurrent diagnosis samples was 27.5%, 53.1%, and 14.3% for ovarian, endometrial, and cervical cancers, respectively. Ovarian and endometrial cancers with a HER3-high recurrent score tended to show shorter median survival time than those with a HER3-low/zero recurrent rating.
Conclusion
Our findings suggest that, in gynecological cancers, the proportion of patients having a HER3-high score increased from initial to recurrent diagnosis.
A Novel Nomogram for Predicting Malignant Cerebral Edema After Endovascular Thrombectomy in Acute Ischemic Stroke: A Retrospective Cohort Study
Authors: He Yuxuan , Zuo Meng , Huang Jialu , Jiang Ying , Zhou Linke , Li Guangjian , Chen Lin , Liu Qu , Liang Dingwen , Wang Yu , Wang Li , Zhou Zhenhua 
Published: October 2022

Abstract

Background
Malignant cerebral edema (MCE) is a common and feared complication after endovascular thrombectomy (EVT) in acute ischemic stroke (AIS). This study aimed to establish a nomogram to predict MCE in anterior circulation large vessel occlusion stroke (LVOS) patients receiving EVT.
Methods
In this retrospective cohort study, 381 patients with anterior circulation LVOS receiving EVT were screened from 636 hospitalized patients with LVOS in two stroke medical centers. Clinical baseline data and imaging data were collected within 2–5 days of admission to the hospital. The patients were divided into two groups based on whether MCE occurred after EVT. Multivariate logistic regression analysis was used to evaluate the independent risk factors for MCE and to establish a nomogram.
Results
Sixty-six patients out of 381 (17.32%) developed MCE. The independent risk factors for MCE included admission NIHSS ≥ 16 (OR, 1.851; 95% CI: 1.029–3.329; P = 0.038), ASPECT score (OR, 0.621; 95% CI: 0.519–0.744; P < 0.001), right hemisphere (OR, 1.636; 95% CI :0.941–2.843; P = 0.079), collateral circulation (OR, 0.155; 95% CI: 0.074–0.324; P < 0.001), recanalization (OR, 0.223; 95% CI: 0.109–0.457; P < 0.001), hematocrit (OR, 0.937; 95% CI: 0.892–0.985; P = 0.010) and glucose (OR, 1.118; 95% CI: 1.023–1.223; P = 0.036), which were adopted as parameters of the nomogram. The receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) of the nomogram in predicting MCE was 0.901(95%CI:0.848–0.940; P < 0.001). The Hosmer-Lemeshow test results were not significant (P = 0.685), demonstrating a good calibration of the nomogram.
Conclusion
The novel nomogram composed of admission NIHSS, ASPECT scores, right hemisphere, collateral circulation, recanalization, hematocrit and serum glucose provide a potential predictor for MCE in patients with AIS after EVT.
Registration:
URL: http://www.chictr.org.cn/; Unique Identifier: ChiCTR2200059412.
A novel mini-invasive step-up approach for the treatment of severe acute pancreatitis with extensive infected necrosis: a single center case series study
Authors: Zhang Beiyuan , Gao Tao , Wang Yan , Zhu Hao , Liu Song , Chen Ming , Yu Wenkui , Zhu Zhanghua 
Published: October 2022

Abstract
Background
Complete removal of necrosis is critical for treating patients with severe acute pancreatitis (SAP) presenting infection of pancreatic necrosis (IPN). Frequently used mini-invasive methods include the surgical step-up approach suitable for necrosis extending laterally, whereas the endoscopic step-up approach is suitable for medial necrosis. This retrospective study evaluated a combined mini-invasive step-up approach for treating extensive IPN.
Methods
Seven patients with SAP and extensive IPN were enrolled. All patients underwent a combined step-up approach comprising four steps: percutaneous catheter drainage (PCD), continuous negative pressure irrigation (CNPI), percutaneous endoscopic necrosectomy (PEN), and transgastric necrosectomy (TN). A lumen-apposing metal stent was used in the TN to shorten the operative time, thereby improving patient tolerance. The details of each step, including timing, frequency, and duration, as well as clinical outcomes of study patients were analyzed.
Results
The median interval from symptom onset to PCD and CNPI was 11 days (range, 6–14) and 18 days (range, 14–26), and the median CNPI duration was 84 days (range, 54–116). The median interval from the onset of symptoms to PEN and TN was 36 days (range, 23–42) and 41 days (range, 34–48), respectively, and the median number of procedures was 2 (range, 1–2) and 3 (range, 2–4). Only a minor case of abdominal bleeding and a pancreatic-cutaneous fistula were reported, both resolved after conservative treatment. The median length of stay in the intensive care unit was 111 days (range, 73–133); all patients survived.
Conclusions
This mini-invasive step-up approach shows promising clinical effects and is relatively safe in critically ill patients with extensive IPN and high-risk surgical intervention. Future clinical studies with larger samples are needed to verify this approach.
Trial registration:
The study protocol was approved by the Medical Ethics Committee of Nanjing Drum Tower Hospital (number:2021-147-01). This study was retrospectively registered in the Clinical Trial Registry (www.clinicaltrials.gov) on August 16, 2022, and the identifier was NCT05508828.
Dietary organic acids on growth, immune response, hepatopancreatic histopathology and disease resistance in Pacific white shrimp, Penaeus vannamei against Vibrio harveyi
Authors: Sivakumar Muniasamy , Amirtharaj K. S. Vijay , Chrisolite B. , Sivasankar P. , Subash Palaniappan 
Published: October 2022

Abstract
The current study was conducted for 75 days to evaluate the impact of Potassium diformate (KDF), Sodium formate (SF) and organic acid blend (KDF + SF) on the growth, immunity and disease resistance in Penaeus vannamei against Vibrio harveyi. Pacific white shrimps (2.11 ± 0.01g) were fed with organic acids and organic acid blend in two different inclusion levels, such as 1 & 2%. After 60 days of feeding trial, the higher body weight gain and survival rate was observed in shrimps fed KDF 2% diet compared to the control groups. Challenge study conducted for 15 days with V. harveyi (108) has shown significantly lower cumulative mortality rate in shrimps fed KDF 2%, KDF 1% and SF 2% treatment groups compared to the control groups. At the end of challenge study, total cultivable bacteria and presumptive vibrio spp. count in shrimp fed KDF2 and all treatments were significantly lower than control diet. The histopathology results were observed with less hepatopancreatic damage in shrimps fed with KDF at 2% level compared to the control. The immune parameters, Total haemocyte count (THC), Prophenoloxidase activity (proPO), Respiratory burst activity (RBA), Superoxide dismutase (SOD), and Catalase activity (CAT) were found to be significantly higher than the control.
Analysis of the Critical Stick-slip Velocity of CNC Machine Tool Combining Friction Parameters Identification and Kinetic Model
Authors: Yang Jianzhong , Zhou Hao , Li Shuo , Chen Jihong , Xiang Hua 
Published: October 2022

Abstract
Stick-slip is a negative phenomenon caused by friction in servo feed systems, which is particularly prominent in low-speed and heavy-load conditions. At present, most research on the critical Stick-slip velocity (CSSV) ignores higher-order terms of the equivalent damping ratio, the calculation accuracy is greatly reduced when the system has a large equivalent damping ratio. Firstly, an Improved Stribeck Model based on Least Squares Genetic algorithm (ISM-LSG) is proposed for friction identification, At the same time, the identification of shape parameters of the traditional Stribeck model is extended instead of empirical values. Then, an improved Critical Velocity solving method based on the kinetic model is constructed, and the influence of the higher-order term of the equivalent damping ratio on the critical velocity identification accuracy is analyzed. Finally, this method is verified by the CNC end-face cylindrical grinder with a hard guideway. The friction force identification error with ISM-LSG is reduced 10% compared with the traditional method, and the error of the critical Stick-slip velocity with retaining higher-order term (CSSV-RHT) is reduced 37% compared with the critical Stick-slip velocity by ignoring higher-order term (CSSV-iHT).
A Study on Multi-agent Reinforcement Learning for Autonomous Distribution Vehicles
Authors: Ergün Serap 
Published: October 2022

Abstract
A self-driving car, also called an autonomous vehicle, is capable of sensing the environment and driving without any human intervention, utilizing software algorithms and a fully automated driving system. This allows the vehicle to manage external conditions similar to a human. Safe and efficient delivery services require road controls and road restrictions to prevent accidents and reduce damage from unexpected technical failures. This study formulates the Autonomous Delivery Vehicles optimization problem and proposes a multi-agent reinforcement learning method using the analytically calculated shortest path information.
Risk Analysis in Design of a Multi-Section Flood Control Levee System: Assessing the Components Coordination and Probability Distribution
Authors: Behrouz Masoume , Alimohammadi Saeed 
Published: October 2022

Abstract
The purpose of this study is risk analysis in optimum design of a composite multi-section flood control levee system along a river reach considering several uncertainties, and assessing the coordination between the system and its individual components. A stochastic optimization model was developed in which Latin Hypercube sampling and Cholesky decomposition was used for quantifying uncertainties. The results indicated that the multi-section model is more cost-effective than a single-section in such a way that total cost of developing system was reduced by 38%. It was also found that with the increase of the complexity of the model (one and multi-section), and with the separation of uncertainties (into epistemic and aleatory), the coordination of the system decreases 130% and 30% respectively. Finally, it was found that lower and upper tails of a component histogram may be completely differ from a fitted distribution.