bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose
Authors: Wang Ling-Ru , Zhang Zi-Xu , Nong Fang-Tong , Li Jin , Huang Peng-Wei , Ma Wang , Zhao Quan-Yu , Sun Xiao-Man 
Published: October 2022

Abstract
Background
Schizochytrium sp. is a heterotrophic, oil-producing microorganism that can efficiently produce lipids. However, the industrial production of bulk chemicals using Schizochytrium sp. is still not economically viable due to high-cost culture medium. Replacing glucose with cheap and renewable lignocellulose is a highly promising approach to reduce production costs, but Schizochytrium sp. cannot efficiently metabolize xylose, a major pentose in lignocellulosic biomass.

Results
In order to improve the utilization of lignocellulose by Schizochytrium sp., we cloned and functionally characterized the genes encoding enzymes involved in the xylose metabolism. The results showed that the endogenous xylose reductase and xylulose kinase genes possess corresponding functional activities. Additionally, attempts were made to construct a strain of Schizochytrium sp. that can effectively use xylose by using genetic engineering techniques to introduce exogenous xylitol dehydrogenase/xylose isomerase; however, the introduction of heterologous xylitol dehydrogenase did not produce a xylose-utilizing engineered strain, whereas the introduction of xylose isomerase did. The results showed that the engineered strain 308-XI with an exogenous xylose isomerase could consume 8.2 g/L xylose over 60 h of cultivation. Xylose consumption was further elevated to 11.1 g/L when heterologous xylose isomerase and xylulose kinase were overexpressed simultaneously. Furthermore, cultivation of 308-XI-XK(S) using lignocellulosic hydrolysates, which contained glucose and xylose, yielded a 22.4 g/L of dry cell weight and 5.3 g/L of total lipid titer, respectively, representing 42.7 and 30.4% increases compared to the wild type.

Conclusion
This study shows that engineering of Schizochytrium sp. to efficiently utilize xylose is conducive to improve its utilization of lignocellulose, which can reduce the costs of industrial lipid production.
Phase Ib Study of Telisotuzumab Vedotin in Combination With Erlotinib in Patients With c-Met Protein–Expressing Non–Small-Cell Lung Cancer
Authors: Camidge D. Ross , Barlesi Fabrice , Goldman Jonathan W. , Morgensztern Daniel , Heist Rebecca , Vokes Everett , Spira Alex , Angevin Eric , Su Wu-Chou , Hong David S. , Strickler John H. , Motwani Monica , Dunbar Martin , Parikh Apurvasena , Noon Elysa , Blot Vincent , Wu Jun , Kelly Karen 
Published: October 2022

PURPOSE Overexpression of c-Met protein and epidermal growth factor receptor ( EGFR) mutations can co-occur in non–small-cell lung cancer (NSCLC), providing strong rationale for dual targeting. Telisotuzumab vedotin (Teliso-V), a first-in-class antibody-drug conjugate targeting c-Met, has shown a tolerable safety profile and antitumor activity as monotherapy. Herein, we report the results of a phase Ib study (ClinicalTrials.gov identifier: NCT02099058 ) evaluating Teliso-V plus erlotinib, an EGFR tyrosine kinase inhibitor (TKI), in patients with c-Met–positive (+) NSCLC. PATIENTS AND METHODS This study evaluated Teliso-V (2.7 mg/kg once every 21 days) plus erlotinib (150 mg once daily) in adult patients (age ≥ 18 years) with c-Met+ NSCLC. Later enrollment required presence of an EGFR-activating mutation ( EGFR-M +) and progression on a prior EGFR TKI. End points included safety, pharmacokinetics, objective response rate (ORR), and progression-free survival (PFS). The efficacy-evaluable population consisted of c-Met+ patients (confirmed histology [H]-score ≥ 150) who had at least one postbaseline scan; c-Met+ patients with H-scores ≥ 225 were classified as c-Met high. RESULTS As of January 2020, 42 patients were enrolled (N = 36 efficacy-evaluable). Neuropathies were the most common any-grade adverse events reported, with 24 of 42 patients (57%) experiencing at least one event. The pharmacokinetic profile of Teliso-V plus erlotinib was similar to Teliso-V monotherapy. Median PFS for all efficacy-evaluable patients was 5.9 months (95% CI, 2.8 to not reached). ORR for EGFR-M + patients (n = 28) was 32.1%. Of EGFR-M + patients, those who were c-Met high (n = 15) had an ORR of 52.6%. Median PFS was 6.8 months for non-T790M+ and for those whose T790M status was unknown, versus 3.7 months for T790M+. CONCLUSION Teliso-V plus erlotinib showed encouraging antitumor activity and acceptable toxicity in EGFR TKI-pretreated patients with EGFR-M+, c-Met+ NSCLC.
The Finnish genetic heritage in 2022 – from diagnosis to translational research
Authors: Uusimaa Johanna , Kettunen Johannes , Varilo Teppo , Järvelä Irma , Kallijärvi Jukka , Kääriäinen Helena , Laine Minna , Lapatto Risto , Myllynen Päivi , Niinikoski Harri , Rahikkala Elisa , Suomalainen Anu , Tikkanen Ritva , Tyynismaa Henna , Vieira Päivi , Zarybnicky Tomas , Sipilä Petra , Kuure Satu , Hinttala Reetta 
Published: October 2022

ABSTRACT
Isolated populations have been valuable for the discovery of rare monogenic diseases and their causative genetic variants. Finnish disease heritage (FDH) is an example of a group of hereditary monogenic disorders caused by single major, usually autosomal-recessive, variants enriched in the population due to several past genetic drift events. Interestingly, distinct subpopulations have remained in Finland and have maintained their unique genetic repertoire. Thus, FDH diseases have persisted, facilitating vigorous research on the underlying molecular mechanisms and development of treatment options. This Review summarizes the current status of FDH, including the most recently discovered FDH disorders, and introduces a set of other recently identified diseases that share common features with the traditional FDH diseases. The Review also discusses a new era for population-based studies, which combine various forms of big data to identify novel genotype–phenotype associations behind more complex conditions, as exemplified here by the FinnGen project. In addition to the pathogenic variants with an unequivocal causative role in the disease phenotype, several risk alleles that correlate with certain phenotypic features have been identified among the Finns, further emphasizing the broad value of studying genetically isolated populations.
Is the Interpolated-Twitch Technique-Derived Voluntary Activation Just Neural? Novel Perspectives from Mechanomyographic Data
Authors: CORATELLA GIUSEPPE , CÈ EMILIANO , DORIA CHRISTIAN , BORRELLI MARTA , TONINELLI NICHOLAS , RAMPICHINI SUSANNA , LIMONTA ELOISA , LONGO STEFANO , ESPOSITO FABIO 
Published: October 2022

ABSTRACT

Purpose
Voluntary activation (VA) determined by interpolation-twitch technique could be affected by the characteristics of the in-series elastic components. To overcome this possible bias, a novel approach based on the mechanomyographic (MMG) signal to detect voluntary activation (VAMMG) has been proposed. We examined the changes in VA and VAMMG after passive stretching to check the influence of neural and mechanical factors in the force output.

Methods
Twenty-six healthy men underwent VA assessment using the interpolated-twitch technique before and after unilateral passive stretching of the plantarflexors (five 45-s on + 15-s off). In addition to the force signal, the MMG signal was detected on gastrocnemius medialis, gastrocnemius lateralis, and soleus. From the force and MMG signal analysis, VA and VAMMG were calculated in the stretched and contralateral nonstretched limbs. Joint passive stiffness was also defined.

Results
In the stretched limb, passive stretching increased dorsiflexion range (mean ± SD = +18% ± 10%, P < 0.001, ES = 1.54) but reduced joint passive stiffness (−22% ± 8%, P < 0.001, ES = −1.75), maximum voluntary contraction (−15% ± 7%, P < 0.001, ES = −0.87), VA (−7% ± 3%, P < 0.001, ES = −2.32), and VAMMG (~−5% ± 2%, P < 0.001, ES = −1.26/−1.14). In the contralateral nonstretched limb, passive stretching increased dorsiflexion range (+10% ± 6%, P < 0.001, ES = 0.80) but reduced joint passive stiffness (−3% ± 2%, P = 0.041, ES = −0.27), maximum voluntary contraction (−4% ± 3%, P = 0.035, ES = −0.24), VA (−4% ± 2%, P < 0.001, ES = −1.77), and VAMMG (~− 2% ± 1%, P < 0.05, ES = −0.54/−0.46). The stretch-induced changes in VA correlated with VAMMG (R ranging from 0.447 to 0.583 considering all muscles) and with joint passive stiffness (stretched limb: R = 0.503; contralateral nonstretched limb: R = 0.530).

Conclusions
VA output is overall influenced by both neural and mechanical factors, not distinguishable using the interpolated-twitch technique. VAMMG is a complementary index to assess the changes in VA not influenced by mechanical factors and to examine synergistic muscles.
The state of individual components of humoral immunity of guinea pig blood in the dynamics of the formation of experimental periodontitis and immobilization stress and correction of their disorders by thiocetam
Authors: Regeda M. , Olekshij P. 
Published: October 2022

The research found a rapid increase in the level of immunoglobulin M, starting from the 3 rd day of the experiment in comparison with the control values, which clearly indicates its activation in particular, and stimulation of humoral immunity in general. In the later stages (on the 5 th and 15 th days) of the formation of experimental periodontitis (EP) and immobilization stress (IS), an increase of this indicator by 93.5% and 94.2% was found, respectively, against group I guinea pigs (p≤0, 05).Regarding the content of immunoglobulin G in the blood, the highest growth rates were recorded in the fourth group of guinea pigs with EP and IS (on the 15 th day), which indicates a direct dependence of the duration of damaging factors on the level of these indicators and the body's ability to the protective response. The results of the treatment showed the effect and the reduction of the activity of the studied classes of immunoglobulins. Thus, the use of thiocetam led to a significant decrease in the content of Ig M and G in the blood, respectively, by 33.8% (p1≤0.05) and 41.7% (p1≤0.05) in EP and IS compared with the group of animals were not exposed to this drug, which indicates the immunocorrective effect of this drug on the studied tests.
Od metafizycznej neutralności do metafizyki problemów. Status metafizyki w filozofii Husserla
Authors: Łaciak Piotr 
Published: October 2022

Od metafizycznej neutralności do metafizyki problemów.
Status metafizyki w filozofii Husserla

Streszczenie

Artykuł prezentuje przejście filozofii Husserla od psychologii fenomenologicznej do fenomenologii transcendentalnej jako zwrot metafizyczny. Husserlowska fenomenologia przedtranscendentalna jako deskryptywna analiza fenomenów mentalnych wyklucza wszelkie metafizyczne pytania dotyczące istnienia świata zewnętrznego, podczas gdy fenomenologia transcendentalna nie jest metafizycznie neutralna, ponieważ ma za zadanie odsłonięcie ostatecznego sensu świata w jego faktycznym istnieniu. Z jednej strony, zgodnie z pierwszeństwem ejdetycznych możliwości przed rzeczywistością, fenomenologia transcendentalna jako nauka ejdetyczna poprzedza metafizykę jako naukę o rzeczywistości i umożliwia ją. Z tego punktu widzenia fenomenologia transcendentalna może być rozumiana w znaczeniu ostatecznego ugruntowania metafizyki jako uniwersalnej i absolutnej nauki o faktach, która jest zwieńczeniem naturalnych nauk o bycie. Z drugiej strony, zasada pierwszeństwa istoty przed faktem nie znajduje zastosowania do transcendentalnego faktu konstytucji świata i ten irracjonalny fakt prowadzi do metafizyki jako filozofii dotyczącej „najwyższych i ostatecznych zagadnień”, takich jak teleologiczne, teologiczne i etyczne zagadnienia. Zagadnienia te okazują się irracjonalną resztą (metafizycznym residuum) fenomenologii transcendentalnej i jej zwieńczeniem.

Słowa kluczowe: ejdetyczne możliwości, fakt absolutny, fenomenologia transcendentalna, metafizyka krytyczna, metafizyka problemów, nauka o faktach
Dwa pokolenia szkoły lwowsko-warszawskiej: Tadeusz Czeżowski (1889-1981) i Zbigniew Jordan (1911-1977)
Authors: Konstańczak Stefan 
Published: October 2022

Artykuł w całości nawiązuje do dotąd niepublikowanej korespondencji Tadeusza Czeżowskiego z emigracyjnym filozofem Zbigniewem Jordanem zachowanej w rękopisach w zasobach archiwalnych Uniwersytetu Mikołaja Kopernika w Toruniu. Ich korespondencja trwała blisko 40 lat i jest dowodem, że nawet przymusowa powojenna emigracja nie zrywała więzów łączących filozofów związanych ze szkołą lwowsko-warszawską. Choć Czeżowski i Jordan należeli do dwóch różnych pokoleń tej szkoły, to wspólne zainteresowania naukowe i podobne zapatrywania polityczne spowodowały, że ich więź, choć tylko korespondencyjna, była w pewnym sensie wyjątkowa, bo nawet ingerencje komunistycznej cenzury nie były w stanie wpłynąć na jej kształt. Dzięki niej Jordan wykazywał się doskonałą orientacją w dorobku samego Czeżowskiego jak i pozostałych przedstawicieli szkoły lwowsko-warszawskiej. To właśnie głównie dzięki tej korespondencji mógł pełnić rolę „ambasadora” polskiej filozofii w świecie i zarazem pierwszego historyka szkoły lwowsko-warszawskiej. Przypomnieniu roli obu filozofów w dziejach polskiej filozofii sprzyja zwłaszcza przypadająca w tym roku 40-ta rocznica śmierci Czeżowskiego oraz 110 rocznica urodzin Jordana.
Wierność prawdzie warunkiem bycia podmiotem moralnym.Porównanie koncepcji Tadeusza Stycznia i Alaina Badiou
Authors: Rejewska Julia 
Published: October 2022

Współczesna etyka coraz rzadziej odwołuje się do kategorii prawdy – z tego powodu zainteresowanie budzić może każda propozycja etyczna, której twórca umieszcza kategorię prawdy w samym jej centrum. Na gruncie polskim taką etyką prawdy jest teoria Tadeusza Stycznia (1931-2010), w której prawda ma charakter normatywny. Do normatywnej mocy prawdy w etyce, choć rozumie on prawdę w zupełnie inny sposób, odwołuje się także francuski filozof Alain Badiou (ur. 1937). Zarówno w myśli Stycznia, jak i Badiou zagadnienie prawdy jest ściśle związane z pojęciem powinności. Nie da się ukryć, że filozofie tych myślicieli są od siebie różne, ale można dostrzec między nimi pewne podobieństwa. Celem tego artykułu jest ukazanie zbieżnych punktów w filozofiach Tadeusza Stycznia i Alaina Badiou. Obaj myśliciele zdają się twierdzić, że człowiek, który dochowuje wierności odkrytej prawdzie czy wydarzeniu, jako podmiot moralny pozostaje wolny, unika zła oraz jest osobą lub Nieśmiertelny.
Commentary on “Frontiers: Spilling the Beans on Political Consumerism: Do Social Media Boycotts and Buycotts Translate to Real Sales Impact?”
Authors: Lelkes Yphtach 
Published: October 2022

Partisan buycotts and boycotts signal a tear in the American social fabric. History: Olivier Toubia served as the senior editor for this article. Conflict of Interest Statement: The author certifies that he has no affiliations with or involvement in any organization or entity with any financial interest or nonfinancial interest in the subject matter or materials discussed in thismanuscript. The author has no funding to report.
Joint Assortment Optimization and Customization Under a Mixture of Multinomial Logit Models: On the Value of Personalized Assortments
Authors: El Housni Omar , Topaloglu Huseyin 
Published: October 2022

Assortment Personalization in E-commerce In the age of big data, online businesses have access to a tremendous amount of browsing and purchasing data from their customers. Consequently, there has been great effort in exploiting this data to offer personalized product assortments to each customer based on what is known about her or his preferences. A personalized assortment can potentially allow enhancing the customer experience as well as improving the revenue of the retailer. In “Joint Assortment Optimization And Customization Under A Mixture Of Multinomial Logit Models: On The Value Of Personalized Assortments,” Omar El Housni and Huseyin Topaloglu consider an optimization model that jointly optimizes the assortment of products carried by an online retailer as well as the personalized assortments offered to each customer. They study the value of personalization and develop algorithms with provable theoretical guarantees to solve the optimization problem.
Chemical and physical restraint use during acute care hospitalization of older adults: A retrospective cohort study and time series analysis
Authors: Jones Aaron , Goodarzi Zahra , Lee Justin , Norman Richard , Wong Eric , Dasgupta Monidipa , Liu Barbara , Watt Jennifer 
Published: October 2022

Background
Chemical and physical restraints are associated with harm in older adults, but our understanding of their use during acute care hospitalizations is limited.

Objectives
To (1) describe restraint use during acute care hospitalizations of older adults at the onset of the COVID-19 pandemic compared to pre-pandemic levels and (2) describe between-hospital variability in restraint use.

Design
Retrospective cohort study with a time series analysis.

Participants
Acute care hospital inpatients, aged 65 years or older, who were discharged from one of four Alberta hospitals or six Ontario hospitals in Canada, between November 1, 2019, and June 30, 2020.

Main measures
We used autoregressive linear models with restricted cubic splines to compare proportions of chemical restraint (that is, psychotropic medications, namely antipsychotics, benzodiazepines, and trazodone) and physical restraint (e.g., mittens) use immediately after the onset of the COVID-19 pandemic with pre-pandemic levels. We describe between-hospital variability in restraint use using intraclass correlation coefficients (ICC) and median odds ratios (OR).

Key results
We included 71,004 hospitalizations. Adjusted for the prevalence of dementia and psychotic disorders, chemical restraint use increased in Ontario hospitals from a pre-pandemic average of 27.1% to 30.8% (p<0.001) before returning to pre-pandemic levels within eight weeks. Physical restraint orders in Ontario increased from 5.9% to 8.3% (p = 0.012) and remained elevated at eight weeks. No significant changes in restraint use were observed in Alberta. There was moderate between-hospital variability in chemical restraint use (ICC 0.041 and median OR 1.43). Variability in physical restraint use was higher (ICC 0.11 and median OR 1.83).

Conclusions
The COVID-19 pandemic impacted in-hospital use of chemical and physical restraints among older adults in Ontario but not Alberta. Substantial differences in chemical and physical restraint use by region and hospital suggests there are opportunities to improve best practices in geriatric care. Future research must support implementation of evidence-informed interventions that standardize appropriate restraint use.
Diagnostic accuracy of contrast-enhanced CT for neck abscesses: A systematic review and meta-analysis of positive predictive value
Authors: Hagelberg Jon , Pape Bernd , Heikkinen Jaakko , Nurminen Janne , Mattila Kimmo , Hirvonen Jussi 
Published: October 2022

Objectives
To review the diagnostic accuracy of contrast-enhanced computed tomography (CT) in differentiating abscesses from cellulitis in patients with neck infections, using surgical findings as the reference standard.

Materials and methods
Previous studies in the last 32 years were searched from PubMed and Embase. Because of partial verification bias (only positive abscess findings are usually verified surgically), sensitivity and specificity estimates are unreliable, and we focused on positive predictive value (PPV). For all studies, PPV was calculated as the proportion of true positives out of all positives on imaging. To estimate pooled PPV, we used both the median with an interquartile range and a model-based estimate. For narrative purposes, we reviewed the utility of common morphological CT criteria for abscesses, such as central hypodensity, the size of the collection, bulging, rim enhancement, and presence of air, as well as sensitivity and specificity values reported by the original reports.

Results
23 studies were found reporting 1453 patients, 14 studies in children (771 patients), two in adults (137 patients), and seven including all ages (545 patients). PPV ranged from 0.67 to 0.97 in individual studies, had a median of 0.84 (0.79–0.87), and a model-based pooled estimate of 0.83 (95% confidence interval 0.80–0.85). Most morphological CT criteria had considerable overlap between abscesses and cellulitis.

Conclusions
The pooled estimate of PPV is 0.83 for diagnosing neck abscesses with CT. False positives may be due to limited soft tissue contrast resolution. Overall, none of the morphological criteria seem to be highly accurate for differentiation between abscess and cellulitis.
Diagnostic efficiency of RPA/RAA integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysis
Authors: Zhang Xiaoyu , Ge Xiao , Shen Fangyuan , Qiao Jinjuan , Zhang Yubo , Li Heng 
Published: October 2022

Objective
To evaluate the diagnostic value of recombinase polymerase/ aided amplification (RPA/RAA) integrated clustered regularly interspaced short palindromic repeats (CRISPR) in the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Methods
We searched relevant literature on CRISPR technology for COVID-19 diagnosis using "novel coronavirus", "clustered regularly interspaced short palindromic repeats" and "RPA/RAA" as subject terms in PubMed, Cochrane, Web of Science, and Embase databases. Further, we performed a meta-analysis after screening the literature, quality assessment, and data extraction.

Results
The pooled sensitivity, specificity and a rea under the summary receiver operator characteristic curve (AUC) were 0.98 [95% confidence interval (CI):0.97–0.99], 0.99 (95% CI: 0.97–1.00) and 1.00 (95% CI: 0.98–1.00), respectively. For CRISPR-associated (Cas) proteins-12, the sensitivity, specificity was 0.98 (95% CI: 0.96–1.00), 1.00 (95% CI: 0.99–1.00), respectively. For Cas13, the sensitivity and specificity were 0.99 (95% CI: 0.97–1.00) and 0.95 (95% CI: 0.91–1.00). The positive likelihood ratio (PLR) was 183.2 (95% CI: 28.8, 1166.8); the negative likelihood ratio (NLR) was 0.02 (95% CI: 0.01, 0.03).

Conclusion
RPA/RAA integrated with CRISPR technology is used to diagnose coronavirus disease-19 (COVID-19) with high accuracy and can be used for large-scale population screening.
Herstories on Screen: Feminist Subversions of Frontier Myths, Kathleen Cummins (2020)
Authors: Chand Ashlynn 
Published: October 2022

Review of: Herstories on Screen: Feminist Subversions of Frontier Myths, Kathleen Cummins (2020)
New York: Columbia University Press, 336 pp., ISBN: 9780231189514 (pbk), $30, ISBN: 9780231189507 (hbk), $95