bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations
Authors: Yenkin Alex L. , Bramley John C. , Kremitzki Colin L. , Waligorski Jason E. , Liebeskind Mariel J. , Xu Xinyuan E. , Chandrasekaran Vinay D. , Vakaki Maria A. , Bachman Graham W. , Mitra Robi D. , Milbrandt Jeffrey D. , Buchser William J. 
Published: October 2022

AbstractMost human genetic variation is classified as variants of uncertain significance. While advances in genome editing have allowed innovation in pooled screening platforms, many screens deal with relatively simple readouts (viability, fluorescence) and cannot identify the complex cellular phenotypes that underlie most human diseases. In this paper, we present a generalizable functional genomics platform that combines high-content imaging, machine learning, and microraft isolation in a method termed “Raft-Seq”. We highlight the efficacy of our platform by showing its ability to distinguish pathogenic point mutations of the mitochondrial regulator Mitofusin 2, even when the cellular phenotype is subtle. We also show that our platform achieves its efficacy using multiple cellular features, which can be configured on-the-fly. Raft-Seq enables a way to perform pooled screening on sets of mutations in biologically relevant cells, with the ability to physically capture any cell with a perturbed phenotype and expand it clonally, directly from the primary screen.
Cftr deletion in mouse epithelial and immune cells differentially influence the intestinal microbiota
Authors: Scull Callie E. , Luo Meng , Jennings Scott , Taylor Christopher M. , Wang Guoshun 
Published: October 2022

AbstractCystic fibrosis (CF) is a life-threatening genetic disorder, caused by mutations in the CF transmembrane-conductance regulator gene (cftr) that encodes CFTR, a cAMP-activated chloride and bicarbonate channel. Clinically, CF lung disease dominates the adult patient population. However, its gastrointestinal illness claims the early morbidity and mortality, manifesting as intestinal dysbiosis, inflammation and obstruction. As CF is widely accepted as a disease of epithelial dysfunction, it is unknown whether CFTR loss-of-function in immune cells contributes to these clinical outcomes. Using cftr genetic knockout and bone marrow transplantation mouse models, we performed 16S rRNA gene sequencing of the intestinal microbes. Here we show that cftr deletion in both epithelial and immune cells collectively influence the intestinal microbiota. However, the immune defect is a major factor determining the dysbiosis in the small intestine, while the epithelial defect largely influences that in the large intestine. This finding revises the current concept by suggesting that CF epithelial defect and immune defect play differential roles in CF intestinal disease.
Atomic-scale tuning of ultrathin memristors
Authors: Goul Ryan , Marshall Angelo , Seacat Sierra , Peelaers Hartwin , Robles Hernandez Francisco C. , Wu Judy Z. 
Published: October 2022

AbstractContinuous device downsizing and circuit complexity have motivated atomic-scale tuning of memristors. Herein, we report atomically tunable Pd/M1/M2/Al ultrathin (<2.5 nm M1/M2 bilayer oxide thickness) memristors using in vacuo atomic layer deposition by controlled insertion of MgO atomic layers into pristine Al2O3 atomic layer stacks guided by theory predicted Fermi energy lowering leading to a higher high state resistance (HRS) and a reduction of oxygen vacancy formation energy. Excitingly, memristors with HRS and on/off ratio increasing exponentially with M1/M2 thickness in the range 1.2–2.4 nm have been obtained, illustrating tunneling mechanism and tunable on/off ratio in the range of 10–104. Further dynamic tunability of on/off ratio by electric field is possible by designing of the atomic M2 layer and M1/M2 interface. This result probes ways in the design of memristors with atomically tunable performance parameters.
Acidic Stigma maydis polysaccharides protect against podocyte injury in membranous nephropathy by maintenance of glomerular filtration barrier integrity and gut-kidney axis
Authors: Wang Xizhu , Dong Yifei , Bao Zhijie , Lin Songyi 
Published: October 2022

MN mice models were induced by C-BSA, and we found that acidic stigma maydis polysaccharides maintained the integrity of the glomerular filtration barrier by promoting slit diaphragm proteins expression and PI3K/AKT signaling.
Toward highly efficient bifunctional electrocatalysts for zinc–air batteries: from theoretical prediction to a ternary FeCoNi design
Authors: Tang Wenhao , He Jialin , Teng Kewei , Gao Lei , Qi Ruiyu , Deng Yirui , Liu Ruiping , Li Ang , Fu Huadong , Wang Chang-an 
Published: October 2022

A novel electrocatalyst of hollow nitrogen-doped carbon tubes with coexisting M–N–C single atoms and alloy nanoparticles (denoted FexCoyNiz@hNCTs) shows excellent bifunctional electrocatalytic performances.
A failure assessment method of island distribution lines based on model‐data hybrid drive
Authors: Li Mingjie , Zheng Yupeng , Tang Bo , Yu Guangzheng , Wang Zhen , Muyeen S. M. 
Published: October 2022

AbstractOpen circuit faults of island distribution lines account for more than 70% of the total number of faults in the distribution network. Failure assessment of lines plays a crucial role in the operational safety of the island distribution network. Aiming at the problems of multiple operational failure factors and difficulty in failure assessment, a failure assessment method of island distribution lines based on model‐data hybrid drive is proposed in this paper. Specifically, in order to accurately describe the actual operating status and failure mechanism of distribution lines in the typical island environment, model‐driven method is used to formulate a comprehensive operational failure index of distribution lines, which combines with the impact of strong wind and corrosion operation scenarios and the operating characteristics of lines. On this basis, to improve the accuracy of failure assessment under the influence of multiple factors in the island environment, an improved light gradient boosting machine model is constructed in a data‐driven manner. Finally, based on actual data from distribution network in Zhoushan, China, the case study reveals that the proposed method could effectively assess the operational failure of island distribution lines.
High‐gain wideband circularly polarised mm‐wave antenna with highly symmetrical radiation patterns
Authors: Xie Xianting , Zhang Long , Mao Chunxu , He Yejun , Gao Steven 
Published: October 2022

AbstractA circularly polarised (CP) antenna with highly symmetrical radiation patterns and wide operating bandwidth is presented for millimetre‐wave (mm‐wave) applications. By loading four parasitic inverted‐F components around a SIW‐fed S‐dipole antenna, better axial ratio (AR) performance and higher gain performance are realised. Furthermore, the proposed antenna can achieve highly symmetrical radiation patterns in different elevation planes within a wide operating band. This unique feature makes it advantageous to more uniform signal coverage. To verify the effectiveness of the antenna, a prototype is fabricated and measured. The experimental results validate that the antenna covers the impedance bandwidth of 49.1% (21.2–35 GHz) and the AR bandwidth of 51.3% (20–33.8 GHz). Besides, the antenna peak gain can reach 10.42 dBic at 28.5 GHz. More importantly, the antenna can radiate extremely symmetrical and uniform waves in different elevation planes within a wide operating band (21–29 GHz). Attributed to these merits, the presented antenna shows great potential in various mm‐wave systems.
Nitrogen and phosphorus‐doped green carbon dots with ‘turn‐off’ fluorometric sensor for Fe3+ ions detection
Authors: Zhang You , Li Mingyang , Ding Yanan , Dai Le , Wu Yue , Zheng Yibo , Li Xiaoguang , Guan Li , Li Xu 
Published: October 2022

AbstractCarbon dots (CDs) were paid more and more attention for detecting ferric ions (Fe3+) containing in the organism or environment. Thus, nitrogen and phosphorus co‐doping green CDs were synthesized using p‐Phenylenediamine as the carbon source and phosphoric acid as phosphorus source by the solvo‐thermal method. As‐prepared CDs were characterized by morphology, optical properties, and surface functional groups. The CDs can emit better green light peak at 511 nm excited by 380 nm UV light. Excitation independence property of N, P‐CDs was determined by measuring the photoluminescence (PL) spectra with different excitation wavelengths. The ions detection ability was investigated by analyzing the emission intensity of CDs in eight different kinds of ions solutions and different concentrations of Fe3+ ions solutions. The N, P‐CDs show high selectivity towards Fe3+ ions with a limit of detection (LOD) of 0.11 μM in the concentration region of 300 to 450 μM. The results illuminated that this kind of CDs can be a candidate material for Fe3+ ions detection.
A comparative investigation of flexible arc suppression devices in distribution network
Authors: Fan Bishuang , Zheng Nan , Zeng Xiangjun , Ma Haihang , Wang Wen , Yu Kun , Zhuo Chao 
Published: October 2022

AbstractWith the rapid development of social economy, power supply reliability of the distribution network has become particularly important. The distribution network has long been faced with problems such as overvoltage, single‐phase grounding failure and grounding protection. Passive, active, and hybrid arc suppression devices (ASDs) are proposed to solve these problems. Passive ASDs have the advantages of high reliability, low control complexity and good economic benefits, and have been widely used in distribution networks. With the wide application of power electronic components in distribution network, the active part and harmonic part of ground current, which cannot be effectively eliminated by passive ASDs, increase sharply. Therefore, an active arc suppression method is proposed. However, due to the high cost and low reliability of the active equipment, the hybrid ASD has better arc suppression effect. In this paper, the existing ASDs are reviewed, and specific simulation models are established to verify the principle and performance of three different ASDs. The advantages and disadvantages of the three ASDs are compared in detail, and the possible problems and research directions in the future research of arc suppression technology are prospected.
Aerodynamic and aeroacoustic properties of axial fan blades with slitted leading edges
Authors: Ocker Christof , Czwielong Felix , Chaitanya Paruchuri , Pannert Wolfram , Becker Stefan 
Published: October 2022

A detailed experimental analysis of the aerodynamic and aeroacoustic properties of flat-plate axial fans with slitted leading edges is performed. The sound emissions of five slitted leading edge designs are measured at a constant rotational speed and at a constant total-to-static pressure rise of the fans. For both cases, the fan blades with slitted leading edges reduce the turbulence interaction noise and lead to a reduction of the overall sound pressure level for volume flow rates above 0.6 m3 s−1 compared to an axial fan with solid leading edges. The far-field noise analysis shows that the slits result in a noise reduction for frequencies below 2 kHz and a noise increase above 2 kHz. In addition, sound source localization is conducted with a microphone array and rotating beamforming methods are applied. The identified sound source distributions prove that slitted leading edges reduce turbulence interaction noise, but generate broadband noise in the fan blades’ trailing edge regions. The maximum sound reduction due to the slits could be detected at a dimensionless frequency of $ fh/\bar{w}\approx 0.5$, where f is the frequency, h is the height of the slit and $ \bar{w}$ is the mean relative inflow velocity. The noise reduction mechanism on axial fan blades corresponds well to previous investigations on flat-plate airfoils with slits.
Acoustic analysis of professional singing masks
Authors: Tur Bogac , Echternach Matthias , Turowski Stefan , Müller Meinard , Köberlein Marie , Döllinger Michael , Kniesburges Stefan 
Published: October 2022

Wearing face coverings became one essential tool in order to prohibit virus transmission during the COVID-19 pandemic. In comparison to speaking and breathing, singing emits a much higher amount of aerosol particles. Therefore, there are situations in which singers can perform or rehearse only if they are using protective masks. However, such masks have a more or less adverse effect not only on the singer’s comfort and tightness of the mask but also on the radiated sound. For this reason, the spectral filtering and directivity of masks designed specifically for professional singing was measured. The tests were performed with a head phantom. Over most of the spectrum, attenuation is observed, although amplification happens at some low frequency bands for different mask types and directions. Especially singing masks with a plastic face shield showed partial amplification of up to +10 dB below a frequency of 2 kHz, while only slight significant attenuation and no amplification (minimal acoustic loss) were seen for woven fabric masks. Above 2.5 kHz, the transparent masks showed the greatest sound attenuation up to −30 dB, while woven fabric masks produced an overall lower sound attenuation of up to −5 dB. In addition at low frequencies, the sound was amplified or attenuated equally in all directions for masks with a stiff plastic face shield. At higher frequencies, the attenuation is higher to the frontal than to the backward direction.
Enhancing Resilience in Family Caregivers Using an mHealth App
Authors: Smeallie Eleanor , Rosenthal Lindsay , Johnson Amanda , Roslin Chloe , Hassett Afton L. , Choi Sung Won 
Published: October 2022

Abstract
Background We previously developed a mobile health (mHealth) app (Roadmap) to promote the resilience of family caregivers during the acute phases of care in patients undergoing hematopoietic cell transplantation (HCT).
Objective This study explored users' perspectives on the uptake of Roadmap's multicomponent features and the app's utility in promoting resilience.
Methods Fifteen participants were randomized to the full version of the app that included resilience-building activities and the other 15 were randomized to the control version that included a limited view of the app (i.e., without any resilience-building activities). They were instructed to use the app for 120 days. Semistructured qualitative interviews were then conducted with users as part of an ongoing, larger Roadmap study (NCT04094844). During the interview, caregiver participants were asked about their overall experiences with the app, frequency of use, features used, facilitators of and barriers to use, and their perspectives on its utility in promoting resilience. Data were professionally transcribed, coded, and categorized through content analysis.
Results Interviews were conducted with 30 participants, which included 23 females and 7 males. The median age of the population was 58 years (range, 23–82). The four main themes that emerged included app use, ease of use, user experiences, and ability to foster resilience. The subthemes identified related to facilitators (convenience and not harmful), barriers (caregiver burden and being too overwhelmed during the acute phases of HCT care), resilience (optimism/positivity and self-care), and app design improvements (personalization and notifications/reminders).
Conclusion The qualitative evaluation provided insights into which components were utilized and how one, or a combination of the multicomponent features, may be enhancing users' experiences. Lessons learned suggest that the Roadmap app contributed to promoting resilience during the acute phases of HCT care. Nonetheless, features that provided enhanced personalization may further improve longer-term engagement.
Distal Hand Migration of Polyacrylamide Gel after Breast Augmentation: A Case Report and Review of the Literature
Authors: Gierej Piotr , Radziszewski Marcin , Miłoński Piotr , Noszczyk Bartłomiej 
Published: October 2022

AbstractBreast augmentation is one of the most popular cosmetic surgery procedures in the world and it requires a comprehensive study of the methods performed. As less-invasive techniques are sought, tissue fillers have found its purpose in these procedures. However, it has been revealed that some of them may be associated with serious complications. One of them is the Aquafilling/Los Deline gel. A case report of a female patient who developed unprecedented sequelae after Aquafilling injection—distant migration of the gel in the hand—was presented in this study. The patient underwent total gel removal from the left forearm, arm, and both breasts as well as wound debridement and irrigation. We discovered a canal connecting the left breast to the left forearm, created by a polyacrylamide hydrogel dislocation. It was thoroughly revised using an endoscope. Despite the advantages of tissue fillers such as simplicity of use and less invasiveness, certain complications can occur after injection. Although a few of them have been banned due to these sequelae, new ones continue to appear. Every new product should be examined very carefully before it is introduced to the market.
A Three-Dimensional Scale for the Qualitative and Quantitative Assessments of Secondary Alveolar Bone Grafting (SABG) in Unilateral Cleft Lip and Palate Patients Using Cone-Beam Computed Tomography (CBCT)
Authors: Kumar Ashish , Batra Puneet , Sharma Karan , Raghavan Sreevatsan , Talwar Aditya , Srivastava Amit , Sood Sundereshwer Chander 
Published: October 2022

Abstract
Background The objective of our study was to derive an objective assessment scale for three-dimensional (3D) qualitative and quantitative evaluation of secondary alveolar bone grafting (SABG) using cone-bone computed tomography (CBCT) in patients with unilateral cleft lip and palate (UCLP).
Methods CBCT scans for pre- and 3-month post-SABG were reviewed for bone volume, height, width, and density of the bony bridge formed in the cleft defect in 20 patients with UCLP. Basic descriptive and principal component analysis was used to extract the various sub-components of the scale. Spearman's correlation was used to check the validity of the scale, and intra-class coefficient (ICC) and Cronbach's α were calculated to establish the reliability and retest applicability of the scale.
Results Each CBCT scan was assessed in five areas: cementoenamel junction (CEJ), root apex, root midpoint, 3 and 6 mm below CEJ, and tabulated in percentiles of 20, 25, 40, 50, 60, and 75 for all the parameters (bone volume, density, and width). These scores were validated when correlated to the scale given by Kamperos et al. Cronbach's α for the domains demonstrated acceptable to excellent internal consistency. The ICC showed good test–retest reliability having a range of scores from 0.89 to 0.94.
Conclusion The proposed scale for the 3D assessment of SABG in patients with UCLP provides gradation for the objective assessment of the bony bridge. This gradation enables the qualitative and quantitative assessments of the bony bridge, thus allowing each clinician to judge SABG more conclusively.
Double Adjacent Ventral Slots in Cervical Disc Extrusion with Epidural Haemorrhage in Four French Bulldogs
Authors: Olender Magdalena , Couturier Jérôme , Couturier Laurent , Gatel Laure , Brissot Hervé 
Published: October 2022

AbstractFour French bulldogs were presented with a history of cervical pain and/or signs of myelopathy and were diagnosed with an acute cervical intervertebral disc extrusion (IVDE), associated with epidural haemorrhage (EH). The lesion was classified as IVDE with EH by a board-certified radiologist based on contrast-enhanced computed tomography images. All dogs were treated with two adjacent ventral slots that confirmed diagnosis. The decompression was assessed by measurement of the amount of residual material on computed tomography studies, and the outcome was evaluated by clinical recheck. All dogs recovered uneventfully after the surgery. This case series describes the first detailed report of extensive cervical IVDE with EH where double adjacent ventral slots was used in a treatment of such lesion.
A Cross-sectional Analysis of Treatment in PUPs in 2021 in Germany – First Data from the GEPHARD Study Group
Authors:   The GEPHARD Investigators,   Contributors:,   Contributors to the cross-sectional analysis:,   Study commission
Published: October 2022

Abstract
Objectives Initial treatment in patients with haemophilia remains challenging. The choice of therapy, timing, dose and frequency have been and are still under intense debate. New treatment options like novel factor concentrates and non-factor therapies broaden the discussion.
Design The German Paediatric Haemophilia Research Database (GEPHARD) is a multicentre prospective observational study including children and adolescents with haemophilia A or B (FVIII or FIX levels <25 IU/dL) in a German treatment centre after January 1st, 2017. A cross-sectional analysis was performed in June 2021.
Results 249 children and adolescents from 22 participating centres in Germany were analysed in this cross-sectional analysis. 203 patients suffered from haemophilia A (PwHA) and 46 from haemophilia B (PwHB). The median age at diagnosis for Pw severe HA or HB was 6 or 2 months, the median age at analysis was 33 or 35 months for Pw severe HA or B, respectively. 117 Pw severe HA received treatment, including plasma derived concentrates (n = 43), standard recombinant concentrates (n = 23), extended half live concentrates (n = 33) and non-replacement therapies (n = 18). For Pw severe HB, plasma derived concentrates (n = 3), standard recombinant concentrates (n = 8) and extended half live concentrates (n = 14) were used. Current inhibitors were reported in 16 PwHA and 1 PwHB.
Conclusions GEPHARD was successfully established as a national cohort for newly diagnosed PwH in Germany. Epidemiological and treatment data were presented. Longitudinal analyses of this growing cohort will allow to value treatment strategies and their outcome in the evolving treatment landscape.
Tuning current plateau regions in parallelized single-electron pumps
Authors: Kim Bum-Kyu , Yu Byeong-Sung , Park Suk-In , Song Jindong , Kim Nam , Bae Myung-Ho 
Published: October 2022

The parallelization of single-electron pumps is a promising method to increase the quantized current level produced from a semiconductor-based single-electron system. In the parallelization of multiple pumps with common gate electrodes, the key process is to fabricate them with high reproducibility, resulting in an overlap of the most accurate regions in current plateaus at the same gate voltages. However, because of the lack of gating reproducibility, we here adopted a separate gate-tuning scheme to realize the overlap of the current plateaus instead of using a common gate scheme. To minimize the number of gates, we used entrance gates in common but an exit gate in separate with an additional in-common gate located outside the quantum dot but near the exit gates. The combination of the additional gate and separate exit gates led to an optimal current plateau overlap with a pair of pumps among six pumps in parallel. Under the optimal plateau-tuned condition, we achieved a relative type-A uncertainty of 1.4 × 10−6 at a 100 pA level with f = 160 MHz in the parallelized mode with the second current plateaus for both pumps.