bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implication of glomerular endothelial expression of interferon-stimulated gene 20, an antiviral effector protein, in human kidney inflammation
Authors: Karasawa Takao , Sato Riko , Imaizumi Tadaatsu , Fujita Masashi , Aizawa Tomomi , Tsugawa Koji , Kawaguchi Shogo , Seya Kazuhiko , Terui Kiminori , Tanaka Hiroshi 
Published: June 2022

Abstract
Background: Besides regulating antiviral response, increased expression of Toll-like receptor 3 (TLR3) in resident renal cells plays a role in the development of some forms of glomerulonephritis. TLR3 activation leads to the production of type I interferon (IFN), which subsequently induces the expression of IFN-stimulated genes (ISGs). We previously reported that in glomerular resident cells, ISG20 expression is regulated via the TLR3/ IFN-β signaling, however, its detailed implications remain undetermined. Methods: Cultured normal human glomerular endothelial cells (GECs) were treated with polyinosinic-polycytidylic acid (poly IC), Escherichia coli lipopolysaccharide (LPS), R848, and CpG (TLR3, TLR4, TLR7, and TLR9 agonists). ISG20, CX3CL1/fractalkine and CXCL10/IP-10 mRNA levels were analyzed using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). ISG20 protein expression was evaluated by western blotting. RNA interference (siRNA) was used to knockdown IFN-β and ISG20. CX3CL1 protein levels were evaluated by enzyme-linked immunosorbent assay (ELISA).Results: In GECs, the expression of ISG20 mRNA and protein was increased by poly IC but not by LPS, R848, or CpG treatment. siRNA-mediated knockdown of IFN-β inhibited the poly IC-induced ISG20 expression. Moreover, ISG20 knockdown prevented the poly IC-induced expression of CX3CL1, a representative chemokine that acts as a strong macrophage chemoattractant, whereas it had no effect on CXCL10 expression. Conclusion: In GECs, ISG20 is regulated via TLR3 but not via TLR4, TLR7, or TLR9 signaling, and ISG20 is involved in regulating CX3CL1 production. Besides regulating the antiviral innate immunity, ISG20 may act as a mediator of CX3CL1 production, thereby inducing glomerular inflammation. Thus, modulating ISG20 expression might be a possible therapeutic strategy for glomerulonephritis.
Relationship between left ventricular mechanical synchrony and left ventricular systolic function: A CZT-SPECT analysis
Authors: Sun Qi ting , Huang Rui liang , Fu Song hai , Wu Chen , Guo Xu liang , Li Tian liang , Hou Yue hong , Wang Fei , Xi Rui , Li Sijin 
Published: June 2022

Abstract
Background: CZT-SPECT has good agreement in the evaluation of mechanical synchronization compared with conventional SPECT.Theaim of this study was to evaluate the correlation between left ventricular mechanical contraction synchrony and left ventricular systolic function by gated myocardial perfusion imaging (GMPI) using Cadmium-Zine-Telluride(CZT)single photon emission computed tomography (SPECT).Methods: This retrospective study involved 371 patients （239 malesand 132 females, mean age 61.06 ± 11.78 years old）who underwent GMPI at the Nuclear Medicine Department of Shanxi Cardiovascular Hospital from January 2020 to August 2020. Systolic synchrony parameters and left ventricular systolic function parameters were calculated via Emory Cardiac Toolbox，includingPP, PSD, PHB, HS, HK, EDV, ESV, and LVEF. Based on LVEF value, patients were divided into the severe reduction group (group 1,127cases, EF<35%), moderate reduction group (group 2, 47 cases, 35% ≤ EF < 45%), mild reduction group (group 3,50 cases, 45%≤EF < 50%) and normal group (group 4 ,147 cases, EF≥50%).Differencesin PP, PSD, PHB, HS and HK between the four groups were compared using one-way ANOVA. Differences between two groups were compared usingLSD-ttest. The correlation among functional and mechanical contraction synchronism factors were analyzed using Pearson test.Results: PP, PSD, PHB, HS and HK were significantly different betweenthe four groups (F=5.20, 188.72, 202.88, 171.05, 101.36, P<0.001). Pairwise comparison tests showed significant differences in PSD and PHB in each two groups, and HS and HK in each two groups except for group 2 and 3 (t=0.28 and 0.39, both P> 0.001). PP was significantly higher in group 1, relative to group 3 (t=2.43, P<0.001) and group 4 (t=3.67, P<0.001). Pearson correlation analysis revealed that LVEF negatively correlats with PP, PSD, PHB (r = 0.194, -0.790, -0.799, all P<0.001). HS and HK showed positive correlation for LVEF (r=0.778 and 0.795, P<0.001), PSD, PHB and ESV were had good positive correlation (r=0.778, 0.795, P<0.001), PSD, PHB and EDV had good positivecorrelation (r=0.722, 0.732, P<0.001). However, PP had poor correlation with EDV (r=0.095, P>0.001). HS and HK were negatively correlated with EDV and ESV (r=-0.700～ -0.594,P<0.001).Conclusion: CZT SPECT GMPI provided left ventricular mechanical contraction synchrony parameters that correlated well with left ventricular systolic function. Worse left ventricular mechanical contraction synchrony lead to decreased LVEF , making the systolic synchrony parameters valuable in the prediction of left ventricular systolic function．
Evaluation of Tuberculosis in Children Using Biological Agent Therapy
Authors: Kara Yalçın , Kızıl Mahmut Can , Nepesov Merve İşeri , Tufan Aslı Kavaz , Çetin Nuran , Aydemir Yusuf , Barış Zeren , Doğan Hilal Kaya , Kılıç Ömer , Dinleyici Ener Çağrı 
Published: June 2022

Abstract
Introduction: Treatment with biological agents, which are classified as monoclonal antibodies, cytokines and fusion proteins, increases patient risk for developing tuberculosis (TB) and non-tuberculous mycobacterial infections. In this study, we aimed to investigate the risk of tuberculosis development in pediatric patients using anti-TNF drugs.Method: One hundred and fifteen pediatric cases who were followed up in Eskişehir Osmangazi University Faculty of Medicine, Department of Pediatrics between January 2011 and December 2021 and received anti-TNF and biological agent treatment were included in the study. The clinical and epidemiological characteristics of the cases were analyzed retrospectively.Results: One hundred and fifteen cases using anti-TNF drugs were included in the study. The mean age of the cases was 13 (2-18) years. Of the cases, 66 (57%) were female and 49 (43%) were male. Of the cases, 76 (66%) had Juvenile Rheumatoid Arthritis, 11 (9.6%) Ulcerative Colitis, 7 (6%) Crohn's, 6 (5.2%) Ankylosing Spondylitis, 5 (%4,3) FMF and 4 of them were followed up due to Psoriasis (3.5%). Etanercept in 74 (64.3%) cases, infliximab in 17 (14.8%) cases, adalimumab in 17 (14.8%) cases, anakinra in 5 (4.3%) cases, and canakinumab was using in 2 (%1.7) cases. PPD was performed in all of the cases and PPD response was measured as <5mm in 89 (77.4%), 5-9 mm in 11 (8.7%), 10-14 mm in 8 (7.4%), >15 mm in 7 (5.6%) cases. Isoniazid (INH) prophylaxis was started for 9 months in 17 cases with the diagnosis of latent tuberculosis. Active tuberculosis was not detected in any of the cases.Conclusion: All patients who are planned to receive anti-TNF therapy should be definitely screened for tüberculosis. Although it is not detected at the beginning of the treatment, regular tuberculosis screening should be continued during the treatment with contact history, symptoms, physical examination, chest X-ray and TST/IGRA in the light of current guidelines
A method for automated drip irrigation and fertigation of crops
Authors: Muthusamy Suresh , Pandiyan Santhiya , Paramasivam Muneeshwari 
Published: June 2022

Abstract
In the agricultural field, continuously monitoring and protecting is an important task for the farmers because it enhances the crop growth. Continuous crop growth and output monitoring is an important feature of agricultural management during the developmental stage. This automated irrigation technique increases the agricultural productivity. In traditional farming method fertilizer for the crops is manually supplied. But, in the modern agriculture technique automating the irrigation system has become essential. There are various irrigation techniques available for the agricultural field. Among these irrigation techniques the most commonly used type of irrigation is the Drip irrigation Method. Nowadays, most of the farmers prefer to use drip irrigation because the quantity of water consumed through drip irrigation is quite low compared with the other irrigation techniques. To move ahead with the drip irrigation technique the process involved in drip irrigation is to be automated fully which does not involves any human intervention. The essential fertilizer to the crops will be supplied with the help of venturi pipes which is known as dripper lines. This method will decrease the man power for supplying fertilizers. Compared to the traditional irrigation system drip irrigation will consume less water. Drip irrigation can also provide water to the plant's roots. Using drip irrigation method the crop will be able to absorb more nutrients compared with the traditional irrigation methods. By automating the drip irrigation system the water consumption for the agricultural field can be further reduced. Various sensors, such as temperature sensors, rain sensors, pH sensors, and humidity sensors, can be used to accomplish this.
Responsiveness of Electromyographically Assessed Skeletal Muscle Inactivity – methodological exploration and implications for health benefits
Authors: Pesola Arto J , Gao Ying , Finni Taija 
Published: June 2022

Abstract
Prolonged sedentary behaviour is detrimental to health due to low contractile activity in large lower extremity muscle groups. This muscle inactivity can be measured with electromyography (EMG), but it is unknown how methodological factors affect responsiveness longitudinally. This study aimed to rank 16 different EMG inactivity thresholds based on their responsiveness (absolute and standardized effect size, responsiveness) using data from a randomized controlled trial targeted at reducing and breaking up sedentary time (InPact, ISRCTN28668090). EMG inactivity duration and usual EMG inactivity bout duration (weighted median of bout lengths) were measured from large lower extremity muscle groups (quadriceps, hamstring) with EMG-sensing shorts. The results showed that the EMG inactivity threshold above signal baseline (3 µV) provided overall the best responsiveness indices. At baseline, EMG inactivity duration of 66.8 ± 9.6% was accumulated through 73.9 ± 36.0s usual EMG inactivity bout duration, both of which were reduced following the intervention (-4.8 percentage points, -34.3 seconds). The proposed methodology can reduce variability in longitudinal designs and the detailed results can be used for sample size calculations. EMG inactivity duration and pattern of accumulation can be measured with appropriate responsiveness, and intervening these outcomes has a potential influence on muscle physiology and health.
Critical role for platelet Ral GTPases in regulating neutrophil extracellular traps and venous thrombosis in mice
Authors: Li Yong , Williams Christopher , Walsh Tony , Brill Alexander , Amulic Borko , Poole Alastair 
Published: June 2022

Abstract
Deep vein thrombosis (DVT) is a multifactorial disease whose incidence is a major global burden on morbidity and mortality rates. While the pathophysiology of the disease is still being uncovered, increasing evidence suggests a more prominent role for platelets than previously suspected. Genetic deletion of both Ral GTPases, RalA and RalB, conditionally in mouse platelets (RalAB-/-), results in a near complete defect in P-selectin externalisation upon activation, while other platelet activation responses and arterial thrombosis were preserved. Using a flow restriction model of DVT, we report that platelet-specific RalAB-/- mice have a near complete ablation of venous thrombosis. Immunohistochemistry analysis revealed that cross sections of thrombi from wildtype (WT) mice consist of an organised scaffolded structure of platelets (CD41 staining) surrounding leukocytes (CD45 staining)/neutrophils (Ly6G staining) producing neutrophil extracellular traps (NETs – CitH3 staining) to stabilize and propagate the thrombus. This organised structure was absent in conditional RalAB-/- thrombi. In vitro analysis of platelet-mediated NET formation was also significantly reduced when platelets lacked RalAB or when platelets were treated with the Ral inhibitor RBC8. In conclusion, we identify platelet Rals as novel critical regulators of venous thrombosis , through their ability to regulate NET formation.
A bloody interaction: Plasma proteomics reveals gilthead sea bream (Sparus aurata) impairment caused by Sparicotyle chrysophrii
Authors: Riera-Ferrer Enrique , Piazzon M. Carla , Pozo Raquel Del , Palenzuela Oswaldo , Estensoro Itziar , Sitjà-Bobadilla Ariadna 
Published: June 2022

Abstract
Background:Sparicotylosis is an endemic parasitic disease across the Mediterranean Sea caused by the polyopisthocotylean monogenean Sparicotyle chrysophrii, which affects the gills of gilthead sea bream (GSB; Sparus aurata). Current disease-management, mitigation and treatment strategies are scarce against sparicotylosis. In order to successfully develop more efficient therapeutic strategies against this disease, understanding which molecular mechanisms and metabolic pathways are altered in the host is critical. This study aims to elucidate how S. chrysophrii infection modulates GSB physiological status and to identify the main altered biological processes through plasma proteomics.Methods:Experimental infections were conducted in a recirculating aquaculture system (RAS), exposing naïve recipient GSB (R; 70 g; N= 50) to effluent water from S. chrysophrii-infected GSB. An additional tank with unexposed naïve fish (C; 70 g; N=50) was maintained in parallel, with open water flow disconnected from the RAS. Haematological and infection parameters from sampled C and R fish were registered for 10 weeks. Plasma samples from R fish were categorised into 3 different groups according to their infection intensity in the right-sided gill arches: low, medium and high (L: 1-25; M: 26-50; H: >50 worms, respectively). Five plasma samples of each category were selected, in addition to five C samples and underwent a SWATH-MS proteome analysis. Additional assays on haemoglobin, cholesterol and the lytic activity of the alternative complement pathway were performed to validate the proteome analysis findings.Results:The discriminant analysis of the plasma protein abundance revealed a clear separation into 3 groups (H, M/L and C). A pathway analysis was performed with the differentially quantified proteins, indicating that the parasitic infection mainly affected pathways related to haemostasis, the immune system and lipid metabolism and transport.Twenty-two proteins significantly correlated with the infection intensity, highlighting apolipoproteins, globins and complement c3. Validation assays in blood and plasma (haemoglobin, cholesterol and lytic activity of alternative complement pathway) confirmed these correlations.Conclusions:Sparicotylosis profoundly alters the haemostasis, the innate immune system and the lipid metabolism and transport in GSB. This study gives a crucial global overview of the pathogenesis of sparicotylosis and highlights new targets for further research.
Introduction of laparoscopic nephrectomy for autosomal dominant polycystic kidney disease as the standard procedure
Authors: Thomas Michael N. , Datta Rabi R. , Wahba Roger , Buchner Denise , Chiapponi Costanza , Kurschat Christine , Grundmann Franziska , Müller Roman , Henze Jörn , Meyer Franziska , Bruns Christiane J. , Stippel Dirk L. 
Published: June 2022

Abstract
Purpose: Autosomal dominant polycystic kidney disease (ADPKD) is a common hereditary disorder and accounts for 5-10% of all cases of kidney failure. 50% of ADPKD patients reach kidney failure by the age of 58 years requiring dialysis or transplantation. Nephrectomy is performed in up to 20% of patients due to compressive symptoms, renal-related complications or in preparation for kidney transplantation. However, due to the large kidney size in ADPKD nephrectomy can come with a considerable burden. Here we evaluate our institution’s experience of laparoscopic nephrectomy (LN) as an alternative to standard-procedure nephrectomy for ADPKD patients.Materials and Methods: We report the results of the first 12 consecutive laparoscopic cystnephrectomies from 08/2020 to 08/2021 in our institution. Intra- and postoperative parameters were collected and analyzed. Health related quality of life (HRQoL) was assessed using the SF36 questionnaire.Results: Median age of patients was 60 years (±8,1a). Mean preoperative kidney volume measured by volumetric analyzes in abdominal CT scan was 3795ml (range 1255-8253ml). Mean operative time was 158min (range 85-227min). Mean postoperative stay was 11 days (range 6-35 days). Only one postoperative complication Clavien-Dindo ³3 occurred (8,3%). SF36 HRQol questionnaire revealed excellent postoperative quality of life after LN.Conclusion: Laparoscopic nephrectomy in ADPKD patients is a safe and effective operative procedure independent of kidney size with excellent postoperative outcomes and benefits of minimally invasive surgery.
New Findings Regarding the Influence of Assistants on Intraoperative Inflatable Penile Prosthesis Complications
Authors: Gross Martin , Wu Shuo-Chieh , Swanton Amanda , Jones James 
Published: June 2022

Abstract
Inflatable penile prosthesis (IPP) implantation is the definitive treatment for refractory erectile dysfunction, yet exposure to this procedure during training of urology residents is often limited. To assess the effects of resident participation in IPP surgery, we compared surgical outcomes in a retrospective case series of 253 IPP surgeries by a single surgeon at the same institution between 2017 and 2020 with the assistance of either a registered nurse first assistant (RNFA) or a resident. Pertinent patient characteristics and surgical complications including device complications, surgical site infection, post-operative bleeding, iatrogenic injury, cardiovascular events, pulmonary events, and urinary retention were documented. Measured outcomes included operative time, Emergency Room (ER) visits, unplanned post-operative visits, pain medication refills, and IPP surgery-associated Clavien-Dindo complications. Compared to RFNAs, resident-assisted IPP surgery was associated with significant increase in mean operative time (71.4 min vs 87.9 min, p<0.01) and post-operative ER visits (3.0% vs 10.6%, p=0.03) but not IPP surgery-associated complications (19.7% vs 20.8%, OR 1.03, 95%CI [0.46 -2.30], multivariate regression) or other measured outcomes. Resident participation did not increase IPP-related complications compared to a dedicated RFNA, supporting the notion that resident assistance in these procedures is appropriate and an important part of training.
Differentiating Callosobruchus of South India with Special Reference to Callosobruchus maculatus - A Useful Guide for Entomologists and Non-Entomologists
Authors: Seram Devina , Natesan Senthil , Muthaiyan Pandiyan , Karthikeyan Adhimoolam , Samuel Kennedy John 
Published: June 2022

Abstract
Losses of seed produce in pulses due to seed beetles is a very serious problem faced by farmers and traders during storage. Resistance (or susceptibility) and the degree of damage in legume seeds differ with respect to different bruchid species. The seed beetles, Callosobruchus chinensis and C. maculatus, are the two most widespread bruchid species in Southern India, which is being potentially misidentified. With this perspective, fundamental studies on their morphology are of great importance for correct identification, species specific resistant variety improvement and management. For a reliable and effective resistance evaluation in breeding program, a known, identified species or biotype must be employed. In this study, dissection of striking external morphological characters of both the species was done for comparisons. Type of antennae and hind femur are two common characters used for distinguishing these allied species. However, the most reliable genus and species indicators are variation in the spines on the underside of hind femur. The possible distinguishing characters of these two widespread species are illustrated in light of previously published literatures including other rare Callosobruchus species found in India viz. C. nigritus and C. orientalis, reported from Manipur, India. Furthermore, Scanning Electron Microscope study of South Indian C. maculatus strain revealed the presence of different types of sensilla on mouthparts and antennal regions, which had different functions. It is expected that this paper will provide practical information to both entomologists and non-entomologists working on bruchids especially plant breeders involved in resistant varietal development in the tropics and sub-tropical countries.
Sound absorption structure and chambers in otoliths of Chinese Bahaba (Bahaba taipingensis): A “Giant Panda” fish species
Authors: Gu Yang-Guang , Huang Hong-Hui , Liang Yan , Fang Yan , Dai Ming , Ou You-Jun , Wang Liang-Gen , Wang Xu-Nuo 
Published: June 2022

Abstract
As a critically endangered (CR) fish species, Chinese Bahaba is a unique “Giant Panda” fish species in China and has been listed among the national first-class wildlife protection animals and China's top 10 genetic resources of aquatic products since 2021. This fish species is of high commercial value because of its swim bladder that is commonly used in traditional Chinese medicine. Its otoliths are the sensory organs immersed in the endolymph for maintaining its balance and hearing. However, rare information has been reported on the sound absorption structure and chambers of otoliths of such “Giant Panda” fish. The big “C” groove was found in the fish’s front sagittal otolith with the crystal cluster in the back sagittal otolith, the former of which is a 3D layered structure, that is constructed by elongated prismatic crystals. Besides, there are numerous small holes and adhesion material in this 3D layered structure, where many chambers were also found, indicating that some specific sounds may be captured by this structure and these chambers may then amplify such sounds at a certain wavelength. This finding could be of great importance for protecting and conserving this critically endangered species.