bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical spectrum transition and prediction model of nonalcoholic fatty liver disease in children with obesity
Authors: Zhou Xuelian , Lin Xiufu , Chen Jingnan , Pu Jiaqi , Wu Wei , Wu Zhaoyuan , Lin Hu , Huang Ke , Zhang Li , Dai Yangli , Ni Yan , Dong Guanping , Fu Junfen 
Published: August 2022

ObjectiveThis study aims to outline the clinical characteristics of pediatric NAFLD, as well as establish and validate a prediction model for the disease.Materials and methodsThe retrospective study enrolled 3216 children with obesity from January 2003 to May 2021. They were divided into obese without NAFLD, nonalcoholic fatty liver (NAFL), and nonalcoholic steatohepatitis (NASH) groups. Clinical data were retrieved, and gender and chronologic characteristics were compared between groups. Data from the training set (3036) were assessed using univariate analyses and stepwise multivariate logistic regression, by which a nomogram was developed to estimate the probability of NAFLD. Another 180 cases received additional liver hydrogen proton magnetic resonance spectroscopy (1H-MRS) as a validation set.ResultsThe prevalence of NAFLD was higher in males than in females and has increased over the last 19 years. In total, 1915 cases were NAFLD, and the peak onset age was 10-12 years old. Hyperuricemia ranked first in childhood NAFLD comorbidities, followed by dyslipidemia, hypertension, metabolic syndrome (MetS), and dysglycemia. The AUROC of the eight-parameter nomogram, including waist-to-height ratio (WHtR), hip circumference (HC), triglyceride glucose-waist circumference (TyG-WC), alanine aminotransferase (ALT), high-density lipoprotein cholesterol (HDL-C), apolipoprotein A1(ApoA1), insulin sensitivity index [ISI (composite)], and gender, for predicting NAFLD was 0.913 (sensitivity 80.70%, specificity 90.10%). Calibration curves demonstrated a great calibration ability of the model.Conclusion and relevanceNAFLD is the most common complication in children with obesity. The nomogram based on anthropometric and laboratory indicators performed well in predicting NAFLD. This can be used as a quick screening tool to assess pediatric NAFLD in children with obesity.
Effect of drying dynamics on the functional structure of a fish assemblage from an intermittent river network
Authors: Gonçalves-Silva Milena , Manna Luisa Resende , Rodrigues-Filho Carlos Alberto Sousa , Teixeira Francisco Keilo , Rezende Carla Ferreira 
Published: August 2022

We are living in a fast-changing world promoted by anthropogenic actions and ecosystems’ functioning has been constantly changed. One example is the globally reduction of river flow that can lead to more than 50% of the rivers and streams around the world becoming intermittent. However, the relationship between environmental changes and biological communities has been limited to taxonomic approach, rather than the functional approach. Functional structure determines how individuals interact with the environment and evaluating the effects of hydrological changes in functional variation can elucidate the responses of aquatic biota under climate changes. Thus, we hypothesized that hydrological phases (dry, re-wetting and wet) would influence both environmental conditions and fish composition (i.e., taxonomic richness and functional structure) in one intermittent river. In addition, we expected that: 1) fish assemblages will have higher values of species richness and Functional Diversity indexes—Richness (FRic), Dispersion (FDis), Specialization (FSpe), and Originality (FOri) in drier phases (dry and re-wetting); and 2) higher values of Functional Diversity indexes related to evenness (FEve) and divergence (FDiv) in the wet phase, due to the predominance of species with high dispersal capacity when sites are connected. Sampling was conducted in the Cruxati river during the three hydrological phases along four sites (250 m each). Functional diversity analysis, involving traits of dispersion, life history and trophic ecology were used to apply Functional Diversity indexes. Indexes were compared from null models and all species were ordered in a multidimensional functional space using principal coordinate analysis (PCoA). In the dry phase, taxonomic richness and FRic between communities is higher, as well as FSpe and FOri, showing that each assemblage has unique characteristics with different strategies allowing the establishment in this hydrological phase. However, communities are less functionally dispersed. In the wet phase, when the river is connected, FDis between communities is higher and species abundances are more equitable with regular distribution in the functional space. Therefore, we conclude that local ecological processes (i.e., dynamics of hydrology) may promote the establishment of species according to their functional traits and thereby the functional structure of local assemblages.
Maternal Xp22.31 copy-number variations detected in non-invasive prenatal screening effectively guide the prenatal diagnosis of X-linked ichthyosis
Authors: Tang Xinxin , Wang Zhiwei , Yang Shuting , Chen Min , Zhang Yue , Zhang Fang , Tan Juan , Yin Ting , Wang Leilei 
Published: August 2022

Background and aims: X-linked ichthyosis (XLI) is a common recessive genetic disease caused by the deletion of steroid sulfatase (STS) in Xp22.31. Maternal copy-number deletions in Xp22.31 (covering STS) can be considered an incidental benefit of genome-wide cell-free DNA profiling. Here, we explored the accuracy and clinical value of maternal deletions in Xp22.31 during non-invasive prenatal screening (NIPS).Materials and methods: We evaluated 13,156 pregnant women who completed NIPS. The maternal deletions in Xp22.31 revealed by NIPS were confirmed with maternal white blood cells by chromosome microarray analysis (CMA) or copy-number variation sequencing (CNV-seq). Suspected positive women pregnant with male fetuses were informed and provided with prenatal genetic counseling.Results: Nineteen maternal deletions in Xp22.31 covering STS were detected by NIPS, which were all confirmed, ranging in size from 0.61 to 1.77 Mb. Among them, eleven women with deletions in male fetuses accepted prenatal diagnoses, and finally nine cases of XLI were diagnosed. The nine XLI males had differing degrees of skin abnormalities, and of them, some male members of ten families had symptoms associated with XLI.Conclusion: NIPS has the potential to detect clinically significant maternal X chromosomal CNVs causing XLI, which can guide the prenatal diagnosis of X-linked ichthyosis and reflect the family history, so as to enhance pregnancy management as well as children and family members’ health management.
Prenatal and postnatal diagnosis of Phelan–McDermid syndrome: A report of 21 cases from a medical center and review of the literature
Authors: Hao Ying , Liu Yang , Yang Jingxin , Li Xingping , Luo Fuwei , Geng Qian , Li Suli , Li Peining , Wu Weiqing , Xie Jiansheng 
Published: August 2022

Background: Phelan–McDermid syndrome (PMS), caused by deletions at 22q13.3 and pathogenic variants in the SHANK3 gene, is a rare developmental disorder characterized by hypotonia, developmental delay (DD), intellectual disability (ID), autism spectrum disorder (ASD), dysmorphic features, absence of or delayed language, and other features.Methods: Conventional karyotyping, chromosomal microarray analysis (CMA), and whole exome sequencing (WES) have been used to detect genetic defects causing PMS. We summarized the genetic and clinical findings from prenatal to postnatal stages of detected cases of PMS and mapped potential candidate haploinsufficient genes for deletions of 22q13. This study aimed to summarize the laboratory findings, genetic defects, and genotype–phenotype correlations for Chinese patients with PMS.Results: Seven prenatal cases and fourteen postnatal cases were diagnosed with PMS in our center. Thirteen cases had a deletion ranging in size from 69 to 9.06 Mb at 22q13.2-q13.33, and five cases had a pathogenic variant or an intragenic deletion in the SHANK3 gene. Three familial cases with a parental carrier of a balanced translocation were noted. A review of the literature noted another case series of 29 cases and a report of five cases of PMS in China. Genotype–phenotype correlations confirmed haploinsufficiency of the SHANK3 gene for PMS and suggested other candidate haploinsufficient genes TNFRSFI3C and NFAM1 genes for immunological features and TCF20, SULT4A1, PARVB, SCO2, and UPK3A genes for intellectual impairment and behavioral abnormality, neurological features, macrocephaly/hypotonia, oculopathy, and renal adysplasia, respectively.Conclusion: Indications for prenatal diagnosis of PMS are not specific, and approximately 85% prenatally diagnosed PMS elected termination of pregnancies after genetic counseling. For postnatal cases, 62.5% were caused by a deletion at 22q13 and 37.5% were caused by a pathogenic variant or an intragenic deletion in the SHANK3 gene. Approximately 6.7% of cases with a deletion were familial, and almost all pathogenic variants were de novo. Combined karyotype, CMA, and WES should be performed to increase the diagnostic yield. The identification of other candidate haploinsufficient genes in deletions of 22q13.2-q13.33 could relate to more severe dysmorphic features, neurologic defects, and immune deficiency. These results provided evidence for diagnostic interpretation, genetic counseling, and clinical management for the Chinese cases of PMS.
Inflammatory perturbations in early life long-lastingly shape the transcriptome and TCR repertoire of the first wave of regulatory T cells
Authors: Yang Juhao , Zou Mangge , Chu Xiaojing , Floess Stefan , Li Yang , Delacher Michael , Huehn Jochen 
Published: August 2022

The first wave of Foxp3+ regulatory T cells (Tregs) generated in neonates is critical for the life-long prevention of autoimmunity. Although it is widely accepted that neonates are highly susceptible to infections, the impact of neonatal infections on this first wave of Tregs is completely unknown. Here, we challenged newborn Treg fate-mapping mice (Foxp3eGFPCreERT2xROSA26STOP-eYFP) with the Toll-like receptor (TLR) agonists LPS and poly I:C to mimic inflammatory perturbations upon neonatal bacterial or viral infections, respectively, and subsequently administrated tamoxifen during the first 8 days of life to selectively label the first wave of Tregs. Neonatally-tagged Tregs preferentially accumulated in non-lymphoid tissues (NLTs) when compared to secondary lymphoid organs (SLOs) irrespective of the treatment. One week post challenge, no differences in the frequency and phenotypes of neonatally-tagged Tregs were observed between challenged mice and untreated controls. However, upon aging, a decreased frequency of neonatally-tagged Tregs in both NLTs and SLOs was detected in challenged mice when compared to untreated controls. This decrease became significant 12 weeks post challenge, with no signs of altered Foxp3 stability. Remarkably, this late decrease in the frequency of neonatally-tagged Tregs only occurred when newborns were challenged, as treating 8-days-old mice with TLR agonists did not result in long-lasting alterations of the first wave of Tregs. Combined single-cell T cell receptor (TCR)-seq and RNA-seq revealed that neonatal inflammatory perturbations drastically diminished TCR diversity and long-lastingly altered the transcriptome of neonatally-tagged Tregs, exemplified by lower expression of Tigit, Foxp3, and Il2ra. Together, our data demonstrate that a single, transient encounter with a pathogen in early life can have long-lasting consequences for the first wave of Tregs, which might affect immunological tolerance, prevention of autoimmunity, and other non-canonical functions of tissue-resident Tregs in adulthood.
A data fusion approach for estimating traffic distribution characteristics of expressway: A case study of guangdong province, china
Authors: Dan Han-Cheng , Ling Chongyu , Zhu Zhiheng , Gao Liansheng , Zeng Xiaojun 
Published: August 2022

Currently, people pay more and more attention to road maintenance, and the traffic characteristics of vehicles play an important role in road quality evolution and maintenance decision, which commonly depends on the collection and analysis of traffic data. Nevertheless, the rationality of traffic data analysis and the scientificity of maintenance decision are deficient. This study carries out a research on the data fusion of multisource traffic data including toll data and video surveillance data. First, the information of vehicle type and axle load is acquired from the toll data, and the lane, speed and temporal information are obtained from the video surveillance data. A Bayesian method is used to train toll data and video surveillance data to recover missing data. The vehicle type distribution probabilities of traffic volume during different periods and speeds in different lanes are investigated. Next, the number of equivalent standard axle load (ESAL) at different lanes, time periods, and speeds are estimated based on the axle load conversion relationship between different vehicle types. Then the axle load spectrum and distribution characteristics of traffic in different sections, lanes, speeds, and time periods are analyzed. Finally, the comparison of rutting depth from the multisource data fusion and specification is carried out, and it shows an apparent difference (e.g., beyond 20%) when the lateral distribution in lanes is taken into account. Although the difference is less than 10% by considering vehicle speed and time periods, the time to reach the same value of rutting depth maybe more than 1 year. Therefore, it greatly affects accurate determination of preventive maintenance timing. As a whole, this study provides beneficial information for accurately understanding the preventive maintenance opportunities and making reasonable maintenance decisions.
A comparative study of functional MRI in predicting response of regional nodes to induction chemotherapy in patients with nasopharyngeal carcinoma
Authors: Zhao Dawei , Fang Xuemei , Fan Wenjun , Meng Lingling , Luo Yanrong , Chen Nanxiang , Li Jinfeng , Zang Xiao , Li Meng , Guo Xingdong , Cao Biyang , Wu Chenchen , Tan Xin , Cai Boning , Ma Lin 
Published: August 2022

PurposeTo identify and compare the value of functional MRI (fMRI) in predicting the early response of metastatic cervical lymph nodes (LNs) to induction chemotherapy (IC) in nasopharyngeal carcinoma (NPC) patients.MethodsThis prospective study collected 94 metastatic LNs from 40 consecutive NPC patients treated with IC from January 2021 to May 2021. Conventional diffusion-weighted imaging, diffusion kurtosis imaging, intravoxel incoherent motion, and dynamic contrast-enhanced magnetic resonance imaging were performed before and after IC. The parameter maps apparent diffusion coefficient (ADC), mean diffusion coefficient (MD), mean kurtosis (MK), Dslow, Dfast, perfusion fraction (PF), Ktrans, Ve, and Kep) of the metastatic nodes were calculated by the Functool postprocessing software. All LNs were classified as the responding group (RG) and non-responding group (NRG) according to Response Evaluation Criteria in Solid Tumors 1.1. The fMRI parameters were compared before and after IC and between the RG and the NRG. The significant parameters are fitted by logistic regression analysis to produce new predictive factor (PRE)–predicted probabilities. Logistic regression analysis and receiver operating characteristic (ROC) curves were performed to further identify and compare the efficacy of the parameters.ResultsAfter IC, the mean values of ADC, MD, and Dslow significantly increased, while MK, Dfast, and Ktrans values decreased dramatically, while no significant difference was detected in Ve and Kep. Compared with NRG, PF-pre and Ktrans-pre values in the RG were higher statistically. The areas under the ROC for the pretreatment PF, Ktrans, and PRE were 0.736, 0.722, and 0.810, respectively, with the optimal cutoff value of 222 × 10-4, 934 × 10-3/min, and 0.6624, respectively.ConclusionsThe pretreatment fMRI parameters PF and Ktrans showed promising potential in predicting the response of the metastatic LNs to IC in NPC patients.Clinical Trial RegistrationThis study was approved by the ethics board of the Chinese PLA General Hospital, and registered on 30 January 2021, in the Chinese Clinical Trial Registry; http://www.chictr.org.cn/showproj.aspx?proj=121198, identifier (ChiCTR2100042863).
Determination of salivary concentrations of leptin and adiponectin, ability to reduce ferric ions and total antioxidant capacity of saliva in patients with severe early childhood caries
Authors: Petrović Bojan , Stilinović Nebojsa , Tomas Ana , Kojić Sanja , Stojanović Goran M. 
Published: August 2022

IntroductionOne of the most common oral diseases affecting children is early childhood caries (ECC). The link between oxidative stress and ECC has been proven in numerous clinical studies. Technical and biological variability were so high in most of the studies that none of the markers have yet been proven suitable for routine clinical use. This study aimed to evaluate the antioxidant status and the levels of leptin and adiponectin in saliva of children with severe early childhood caries (S-ECC).MethodsMorning unstimulated saliva samples were collected from children (n = 40, 0–6 years old) for the evaluation of oxidative stress which were measured by total antioxidant capacity (TAC), and by the ferric reducing antioxidant power (FRAP) assays, as well as to assess the salivary levels of leptin and adiponectin. FRAP, TAC, leptin and adiponectin concentrations were evaluated in S-ECC group (n = 31) and caries free group CF (n = 9). All results were analyzed based on age and sex.ResultsOverall median salivary leptin and adiponectin levels were 5.59 pg/mL and 24.86 ng/mL, respectively. Significantly lower leptin levels were observed in saliva of caries free children (4.66 pg/mL) than in the S-ECC group (6.64 pg/mL, p &lt; 0.01). No significant difference was observed for adiponectin levels (S-ECC and CF, 25.31 and 23.2 ng/mL, respectively, p = 0.961). TAC and FRAP values of saliva had similar values in children with S-ECC and caries free children. TAC and FRAP values also remained stable with the age of the children, without significant differences with respect to sex.ConclusionThe increased concentrations of leptin in saliva of children with S-ECC suggests that leptin may play a role in inflammatory and immune responses in the development of early childhood caries.
A study of the top-cited studies on drug therapy for HIV
Authors: Tang Jie , Yuan Yanwen , Sun Lei , Wu Bo , Yu Lin 
Published: August 2022

Background Research on drug therapy for HIV remained major hot-spots, but relevant data were not satisfactory. We aimed to assess the status and trends of the most cited studies on drug therapy for HIV by using bibliometric methods.Methods The Web of Science Core Collection database was searched for the drug therapy for HIV studies. The period for retrieval was from the beginning of the database to July 26, 2022. The 100 top cited studies were selected. These general information and bibliometric data were collected and analyzed. VOS viewer software was used for visualization analysis.Results The number of citations for the 100 top cited studies ranged from 451 to 5597 and were published from 1987 to 2017. These studies were published in 29 journals. The top 3 journals in terms of the number of studies were the New England Journal of Medicine (n = 22), Lancet (n = 15), and JAMA (n = 13). The most frequently nominated author was Matthias Eiger from the University of Bern, who has contributed 5 studies. United States, Switzerland, and England contributed most of the highly cited studies. Research hot spots reflected clinical trials, treatment adverse events, basic research, and clinical adherence.Conclusion The majority of 100 top-cited studies have been published in the United States, and primarily focused on treatment adverse events, basic research, and clinical adherence. They provide a basic list of the most important and influential academic contributions to literature of HIV drug treatment for researchers.
Functional and Traditional Resistance Training Are Equally Effective in Increasing Upper and Lower Limb Muscular Endurance and Performance Variables in Untrained Young Men
Authors: Zuo Chongwen , Bo Shumin , Wang Tao , Zhang Wei 
Published: August 2022

Background: Functional resistance training (FRT) has been proposed as a safe alternative to traditional resistance training (TRT) for developing neuromuscular adaptation capacity and improving muscular strength and competitive performance. This study sought to compare the effects of 6 weeks of FRT and TRT on upper and lower limb muscular endurance and performance variables in untrained young men.Methods: Twenty-nine untrained healthy young males aged 18–29 years were randomly given 6 weeks of FRT [40% of 1repetition maximum (RM), 4,5 sets of 20 repetitions, 3 times/week] or TRT (70% of 1RM, 4,5 sets of 12 repetitions, 3 times/week). All participants underwent numerous tests before and after the 6-week training, such as muscular endurance (reps of bench press and leg flexion) and physical performance tests (sprint performance, pull-ups, throwing ability, and jumping ability).Results: After the 6 weeks of training, the TRT and FRT groups showed an equally significant increase in muscular endurance (p &lt; 0.01), while the throwing and jumping abilities, 30-m sprint, and pull-ups performances in both the groups (p &lt; 0.01) also improved significantly. However, no differences were observed between the groups (p &gt; 0.05).Conclusion: These findings indicate that both functional resistance training and traditional resistance training are effective training methods for improving the upper and lower limb muscular endurance and performance in untrained young men.