bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alternate response of Bacillus anthracis atxA and acpA to serum, HCO3- and CO2
Authors: Glinert Itai , Bar-David Elad , Ben-Shmuel Amir , Sittner Assa , Puni Reut , Laredo Shira , Kobiler David , Weiss Shay , Levy Haim 
Published: February 2022

AbstractBacillus anthracis overcomes host immune responses by producing capsule and secreting toxins. Production of these virulence factors in response to entering the host environment was shown to be regulated by atxA, the major virulence regulator, known to be activated by HCO3- and CO2. While toxin production is regulated directly by atxA, capsule production is independently mediated by two regulators; acpA and acpB. In addition, it was demonstrated that acpA has at least two promotors, one of them shared with atxA. We used a genetic approach to study capsule and toxin production under different conditions. Unlike previous works utilizing NBY-HCO3- medium under CO2 enriched conditions, we used a sDMEM-based medium. Thus, toxin and capsule production can be induced in ambient or CO2 enriched atmosphere. Using this system, we could differentiate between induction by 10% NRS, 10% CO2 or 0.75% HCO3-. In response to high CO2, capsule production is induced by acpA in an atxA-independent manner, with little to no toxin (protective antigen PA) production. atxA is activated in response to serum independently of CO2, inducing toxin and capsule production in an acpA or acpB dependent manner. HCO3- was also found to activate atxA, but in non-physiological concentrations. Our findings may help explain the first stages of inhalational infection, in which spores germinating in dendritic cells require protection (by encapsulation) without affecting cell migration to the draining lymph-node by toxin secretion.
Aberrant cortical development is driven by impaired cell cycle and translational control in aDDX3Xsyndrome model
Authors: Hoye Mariah L. , Calviello Lorenzo , Poff Abigail J. , Ejimogu Nna-Emeka , Newman Carly R. , Ou Jianhong , Floor Stephen N. , Silver Debra L. 
Published: February 2022

AbstractMutations in the RNA helicase,DDX3X, are a leading cause of Intellectual Disability and present asDDX3Xsyndrome, a neurodevelopmental disorder associated with cortical malformations and autism. Yet the cellular and molecular mechanisms by whichDDX3Xcontrols cortical development are largely unknown. Here, using a mouse model ofDdx3xloss-of-function we demonstrate that DDX3X directs translational and cell cycle control of neural progenitors, which underlies precise corticogenesis. First, we show brain development is highly sensitive toDdx3xdosage; CompleteDdx3xloss from neural progenitors causes microcephaly in females, whereas hemizygous males and heterozygous females show reduced neurogenesis without marked microcephaly. In addition,Ddx3xloss is sexually dimorphic, as its paralog,Ddx3y,compensates forDdx3xin the developing male neocortex. Using live imaging of progenitors, we show that DDX3X promotes neuronal generation by regulating both cell cycle duration and neurogenic divisions. Finally, we use ribosome profilingin vivoto discover the repertoire of translated transcripts in neural progenitors, including those which are DDX3X-dependent and essential for neurogenesis. Our study reveals invaluable new insights into the etiology ofDDX3Xsyndrome, implicating dysregulated progenitor cell cycle dynamics and translation as pathogenic mechanisms.
Phosphatidic acid inhibits inositol synthesis by inducing nuclear translocation of IP6K1 and repression of myo-inositol-3-P synthase
Authors: Lazcano Pablo , Schmidtke Michael W. , Onu Chisom , Greenberg Miriam L. 
Published: February 2022

ABSTRACTInositol is an essential metabolite that serves as a precursor for structural and signaling molecules. Although perturbation of inositol homeostasis has been implicated in numerous human disorders, surprisingly little is known about how inositol levels are regulated in mammalian cells. A recent study in mouse embryonic fibroblasts (MEFs) demonstrated that nuclear translocation of inositol hexakisphosphate kinase 1 (IP6K1) mediates repression of myo-3-P synthase (MIPS), the rate-limiting inositol biosynthetic enzyme. Binding of IP6K1 to phosphatidic acid (PA) is required for this repression. The current study was carried out to elucidate the role of PA in IP6K1 repression. The results indicate that increasing PA levels through pharmacological stimulation of phospholipase D (PLD) or direct supplementation of 18:1 PA induces nuclear translocation of IP6K1 and represses expression of MIPS protein. This effect was specific to PA synthesized in the plasma membrane, as ER-derived PA did not induce IP6K1 translocation. PLD-mediated PA synthesis can be stimulated by the master metabolic regulator 5’ AMP-activated protein kinase (AMPK). Activation of AMPK by glucose deprivation or by treatment with the mood stabilizing drugs valproate (VPA) or lithium recapitulated IP6K1 nuclear translocation and decreased MIPS expression. This study demonstrates for the first time that modulation of PA levels through the AMPK-PLD pathway regulates IP6K1-mediated repression of MIPS.
The Zpr-3 antibody recognizes the 320-354 region of Rho and labels both rods and green cones in zebrafish
Authors: Gao Pan , Qin Yayun , Qu Zhen , Huang Yuwen , Liu Xiliang , Li Jingzhen , Liu Fei , Liu Mugen 
Published: February 2022

AbstractRetinal markers with high quality and specificity are important for the observation of pathologic changes of retinal cells during retinal development, degeneration, and regeneration. Zpr-3 is a mouse monoclonal antibody with uncharacterized antigen usually used to label rod photoreceptors. In this study, we provided evidence to demonstrate that the antigen gene of zpr-3 is rho, which encodes the rod opsin, and the exact epitope of zpr-3 is the 320-354 region of Rho protein. More importantly, our immunofluorescence assays indicated that zpr-3 labels both the outer segments of rods and green-cones on zebrafish retinal sections, probably due to the cross-reaction with the green-cone opsin. Our work is valuable for the scientific community to interpret the experimental data involving the zpr-3 antibody.
Katnip is required to maintain microtubule function and lysosomal delivery to autophagosomes and phagosomes
Authors: Starling Georgina P. , Philips Ben A , Ganesh Sahana , King Jason S. 
Published: February 2022

AbstractThe efficient delivery of lysosomes is essential for many cell functions, such as the degradation of unwanted intracellular components by autophagy and the killing and digestion of extracellular microbes within phagosomes. Using the amoeba Dictyostelium discoideum we find that cells lacking Katnip (Katanin interacting protein) have a general defect in lysosome delivery and whilst able to make autophagosomes and phagosomes correctly are then unable to digest them.Katnip is largely unstudied yet highly conserved across evolution. Previously studies found Katnip mutations in animals cause defects in cilia structure. Here we show that Katnip plays a general role in maintaining microtubule function. We find that loss of Katnip has no overall effect on microtubule dynamics or organisation, but is important for the transport and degradation of endocytic cargos. Strikingly, Katnip mutants become highly sensitive to GFP-tubulin expression, which leads to microtubule tangles, defective anaphase extension and slow growth. Our findings establish a conserved role for Katnip in the function of all microtubules, not just cilia as previously reported. We speculate this is via a key function in microtubule repair, required to maintain endosomal trafficking and lysosomal degradation.
Improvement of aircraft maintenance manual (AMM) for Cessna 172
Authors: Gunes Tarik , Turhan Ugur , Yörük Açıkel Birsen 
Published: February 2022

Purpose
This study aims to bring a new approach to the existing structure of Cessna 172 type Aircraft Maintenance Manuals (AMM), operational safety, suitability for maintenance operations and human factors. The purpose of this study is to maintain maintenance operations more safely and effectively.

Design/methodology/approach
This study tried to develop an approach by using focus group interview and individual interview techniques. At all stages of the study, interviews were made and support was received from the maintenance technicians, technical instructors and students from the aviation industry stakeholders.

Findings
According to the answers given by the participants, the new approach could have a positive effect on operational safety (98.71%), could have a positive effect on human performance (95.72%) it is understandable (93.16%), it is proper for maintenance operations (88.74%) with high potential to convert into practice (85.92%) and a high potential for future applications (97.06%).

Practical implications
It can be used in aviation organizations that operate AMM Cessna 172 type aircraft, created with the new approach, in maintenance aircraft maintenance enterprises and maintenance training institutions. Thanks to this approach, aircraft maintenance technicians will be able to perform safely and more effective maintenance activities.

Originality/value
Contrary to the technologies used by organizations that host wide-body aircraft in their fleets, it requires less cost and less workload to create, use and update. Low-cost airline organizations and maintenance training institutions will also be able to achieve this approach.
Who loves to forgive? The mediator mechanism of service recovery between brand love, brand trust and purchase intention in the context of food-delivery apps
Authors: Aureliano-Silva Leonardo , Spers Eduardo Eugênio , Lodhi Rab Nawaz , Pattanayak Monalisa 
Published: February 2022

PurposeThis study investigates the mediating role of service recovery between brand love (BL), brand trust (BT) and purchase intention in the context of food-delivery apps.Design/methodology/approachThis study follows a quantitative approach. The authors conducted an online survey and collected 275 responses from users of food-delivery apps in South America and Asia. The authors analyzed the conceptual model proposed using structural equation modeling (SEM) in Smart PLS 3.0.FindingsThe results showed a direct and significant relationship between brand love, BT and purchase intention. Additionally, the authors identified the mediating role of service recovery between brand love, BT and purchase intention.Research limitations/implicationsFirst, this study focused on the service recovery construct in general. Future research can address different types of service recovery, for example, core, interpersonal and procedure failures (Kim and Jang, 2016). Second, the authors restricted the study to the relationship between brand love, BT and purchase intention. Future studies can include other constructs, such as e-word of mouth, loyalty and information risk, as intervening variables. A larger sample can also be considered to support the generalization of the findings.Practical implicationsThis study recommends that companies enchant customers with immediate actions after a service failure has occurred. In doing so, companies must monitor those customers who have experienced a service failure, measuring the level of trust in the branded app and checking the frequency of purchases after a service recovery. Interacting with customers through messages is also an important action to manage their purchase intention following the problem's solution. Furthermore, companies must segment customers who have experienced a failure and direct them to specific benefits to reinforce their trust in the app. Then, after correcting the problem, they should pay them special attention by offering benefits, like discounts, coupons and free delivery, as a strategy to promote future purchases.Originality/valueThis is the first paper to investigate the impact of service recovery on brand love, BT and purchase intention in the context of food-delivery services. The authors extend the knowledge about consumers' responses in the case of a failure caused by consumers' loved brands and show how service recovery actions can establish BT and influence future purchases.
Circular economy-based reverse logistics: dynamic interplay between sustainable resource commitment and financial performance
Authors: Fernando Yudi , Shaharudin Muhammad Shabir , Abideen Ahmed Zainul 
Published: February 2022

PurposeThe study aims to propose a circular economy-based reverse logistics (CERL) that emphasises the mediation effect of reverse logistics (RL) on sustainable resource commitment and financial performance.Design/methodology/approachThe structural equation modelling (SEM) approach has been applied to analyse the data acquired through the survey method that included 113 vendors of automotive supplies of the 1st and 2nd levels.FindingsThe results confirm that CERL acts as an essential intervening entity between resources and financial performance. The findings of the study have provided research and development (R&D) opportunities for the industries to find alternative revenue streams and generate profit from resource investment whilst upholding environmental standards through reverse logistic practices.Practical implicationsReverse logistic practices are the key components of a circular business model and a sustainable supply chain. The manufacturing companies need to explore critical enablers that can contribute to business productivity and financial growth.Originality/valueThe study has validated a CERL model that portrays the circular economy's resilient relationship with RL practices.
Intellectual capital and innovation performance: the moderating role of big data analytics: evidence from the banking sector in Jordan
Authors: AL-Khatib Ayman Wael 
Published: February 2022

PurposeThe purpose of this study is to identify the impact of intellectual capital on the innovation performance of the Jordanian banking sector and identify the moderating role of big data analytics.Design/methodology/approachFor this study's purposes, 333 questionnaires were analysed. Convergent validity, discriminant validity and reliability tests were performed through structural equation modelling (SEM) in the Smart-PLS program. A bootstrapping technique was used to analyse the data.FindingsEmpirical results showed that each of the components of intellectual capital and big data analytics explains 63.5% of the variance in innovation performance and that all components of intellectual capital have a statistically significant impact on innovation performance. The results also revealed that the relationship between structural capital and innovation performance is moderated through big data analytics.Research limitations/implicationsThis cross-sectional study provides a snapshot at a given moment in time, a methodological limitation that affects the generalisation of its results, and the results are limited to one country.Practical implicationsThis study promotes the idea of focusing on components of intellectual capital to enhance innovation performance in the Jordanian banking sector and knowing the effect of big data analytics in this relationship.Social implicationsThis study makes recommendations for financial policymakers to improve the effectiveness of intellectual capital practices and innovation performance in the context of big data analytics.Originality/valueThis study has important implications for leaders in the Jordanian banking sector, in general, as the study highlights the importance of intellectual capital to enhance the innovation performance, especially in light of the big data analytics in this sector, and thus increase the innovative capabilities of this banks, which leads to an increase in the level of innovation.
Multilayer grey situation group decision-making model based on cooperative game
Authors: Zhang Na , Yan Shuli 
Published: February 2022

PurposeIn the process of group decision-making, there may be multilayer subjects. In other words, members of the decision-making group may come from different layers and there is interest game among decision experts. Therefore, it is an extremely important topic to aggregate the information of decision experts who are involved in hierarchical relations and gaming relations so as to effectively address game conflicts and reach game cooperation.Design/methodology/approachFirst, a programming model is established to minimize the difference of expert opinions in hierarchical decision-making, and the method to solve the optimal solution is given. Second, the cooperative game model and its properties are discussed by using cooperative game and Shapley value, and the method to determine the weight vector between layers is also proposed.FindingsThis model can quickly aggregate information and achieve game equilibrium among decision-makers with hierarchical relationships. It can be widely used in decision evaluation with hierarchy structure and has certain practical value.Originality/valueIn order to solve the problem that experts at different levels may have conflicts of interest in multilayer grey situation group decision-making process, cooperative game and Shapley value theory are introduced into the study, and a multilayer grey situation group decision-making model based on cooperative game is constructed. The validity and practicability of the model are illustrated by an example.
Islamic community-based business cooperation and sustainable development goals: a case of pesantren community in Indonesia
Authors: Zaki Irham , Zusak M. Bastomi Fahri , Mi'raj Denizar Abdurrahman , Hasib Fatin Fadhilah 
Published: February 2022

Purpose
This study aims to discuss the model of community-based cooperation between pesantren businesses, using case study approach toward an Indonesian pesantren community.

Design/methodology/approach
The data in this research are gathered through through in-depth interviews with Hebitren managements and forum group discussion with the stakeholders of the Hebitren, which includes Hebitren managements themselves, Bank Indonesia and several related ministries.

Findings
The results show that pesantren business cooperations in Indonesia is related in Sustainable Development Goals (SDGs). This cooperation model is expected to be able to contribute in the effort to accomplish SDGs, which include: no poverty (SDGs 1), quality education (SDGs 4) and partnership for the goals (SDGs 17).

Research limitations/implications
The models of pesantren business cooperation are not comprehensive because of the various characteristics of pesantren and its model business. Moreover, Hebitren is one of national forum for business pesantren. So, that the results are possibly applicable.

Practical implications
This research highlights the importance of pesantren’s business unit cooperation management by improving the quality of human resources and business’s infrastructures. This study is a new research model that comprehensively discusses the pesantren community as a form of economic and business development.

Originality/value
To the best of the authors’ knowledge, this paper is the first model of inter-pesantren business unit network in a pesantren business community comprehensively.
Women’s experiences of prison-based mental healthcare: a systematic review of qualitative literature
Authors: Bright Ann-Marie , Higgins Agnes , Grealish Annmarie 
Published: February 2022

Purpose
The rate of female committals to prison has grown rapidly in recent years. Women in prison are likely to have trauma histories and difficulties with their mental health. This paper aims to synthesise the findings of qualitative literature to gain a deeper understanding of the experiences of women in the context of prison-based mental health care.

Design/methodology/approach
A systematic search of five academic databases, Cumulative Index to Nursing and Allied Health Literature, Applied Social Sciences Index and Abstracts, Psychological Information Database (PsycINFO), Excerpta Medica DataBASE (EMBASE) and Medline, was completed in December 2020. This study’s search strategy identified 4,615 citations, and seven studies were included for review. Thomas and Harden’s (2008) framework for thematic synthesis was used to analyse data. Quality appraisal was conducted using the Joanna Briggs Institute Checklist for Qualitative Research (Lockwood et al., 2015).

Findings
Four analytic themes were identified that detail women’s experiences of prison-based mental health care: the type of services accessed and challenges encountered; a reduction in capacity to self-manage mental well-being; the erosion of privacy and dignity; and strained relationships with prison staff. There is a paucity of research conducted with women in the context of prison-based mental health care. The findings suggest there is a need for greater mental health support, including the need to enhance relationships between women and prison staff to promote positive mental health.

Originality/value
To the best of the authors’ knowledge, this is the first systematic review conducted on the experiences of women in the context of prison-based mental health care.
University air travel and greenhouse gas mitigation: an analysis of higher education climate policies
Authors: Schmidt Anthony 
Published: February 2022

Purpose
Numerous higher education institutions have created policies that aim to reduce their carbon footprints. Most policies focus on reducing greenhouse gas emissions related to energy production and consumption. One area that has received less attention has been greenhouse gas emissions from university air travel. The purpose of this paper is to understand how US higher education institutions address university air travel emissions.

Design/methodology/approach
The present research used qualitative document analysis to examine the climate policies of 44 doctoral institutions. The analysis sought to establish themes across a range of climate policy documents from the sampled institutions. Intercoder consensus, peer review and member checking were used to increase the reliability and validity of the analysis.

Findings
Five major themes emerged from the documents: no consideration of air travel, lack of quality data for accurate consideration, recommendations to offset air travel emissions, support for videoconferencing and other suggestions for mitigation. These themes are discussed in detail, as are practical suggestions and implications stemming from this and related research.

Research limitations/implications
The research is based on a sample of US doctoral institutions and their public documents. It is therefore limited in its generalizability.

Practical implications
Institutions need to create a culture in which individual behavior changes toward lower travel are supported. Though problematic, institutions in the USA need to strive to implement suggested offset programs. Given the ubiquity of virtual presence, institutions need to further support videoconferencing.

Originality/value
To the best of the author’s knowledge, this paper is among the first to examine how higher education institutions in the USA address air travel emissions.