bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term vaccine efficacy of a 2-dose varicella vaccine in China from 2011 to 2021: A retrospective observational study
Authors: Shu Mingming , Zhang Dandan , Ma Rui , Yang Tianchi , Pan Xingqiang 
Published: November 2022

ObjectiveA 2-dose varicella vaccine immunization strategy has been implemented in many cities in China, but there is few evidence on a long-term evaluation of the efficacy of the 2-dose varicella vaccine from China. This study aims to assess the long-term vaccine efficacy of the two doses varicella vaccine and analysis of its influencing factors.MethodsA retrospective study was carried out in 837,144 children born between 2011 and 2017 in Ningbo, Easten China. The logistic regression was performed to estimate varicella vaccine effectiveness (VE).ResultsThe overall VE of 2 doses of varicella vaccine compared without the vaccine was 90.31% (89.24–91.26%), and the overall incremental VE of 2 doses of varicella vaccine compared to the 1-dose was 64.71% (59.92–68.93%). Moreover, the varicella vaccination age of the second dose and the interval between 2 doses were both associated with VE. The VE compared to that without the vaccine in children vaccinated at &lt;4 years old was 91.22% (95%CI: 90.16–92.17%) which was higher than in children vaccinated at ≥4 years old (VE: 86.79%; 95%CI: 84.52–88.73). And the effectiveness of the vaccine was 93.60% (95%CI: 92.19–94.75%) in children with the interval of the 2 doses ≤ 24 months significantly higher than in children with the interval of ≥36 months (VE: 85.62%, 95%CI: 82.89–87.91%).ConclusionsThis study provides evidence for long-term VE of the 2-dose varicella vaccine and the better age for 2-dose vaccination and the interval between 2 doses of the vaccine in China.
Real-time mortality statistics during the COVID-19 pandemic: A proposal based on Spanish data, January–March, 2021
Authors: Equiza-Goñi Juan 
Published: November 2022

ObjectivesDuring the COVID-19 pandemic, surveillance systems worldwide underestimated mortality in real time due to longer death reporting lags. In Spain, the mortality monitor “MoMo” published downward biased excess mortality estimates daily. I study the correction of such bias using polynomial regressions in data from January to March 2021 for Spain and the Comunitat Valenciana, the region with the highest excess mortality.MethodsThis adjustment for real-time statistics consisted of (1) estimating forthcoming revisions with polynomial regressions of past revisions, and (2) multiplying the daily-published excess mortality by these estimated revisions. The accuracy of the corrected estimates compared to the original was measured by contrasting their mean absolute errors (MAE) and root mean square errors (RMSE).ResultsApplying quadratic and cubic regressions improved the first communication of cumulative mortality in Spain by 2–3%, on average, and the flow in registered deaths by 20%. However, for the Comunitat Valenciana, those corrections improved the first publications of the cumulative mortality by 36–45%, on average; their second publication, by 23–30%; and the third, by 15–21%. The flow of deaths registered each day improved by 62–63% on their first publication, by 19–36% on the second, and by 12–17% on the third.ConclusionIt is recommended that MoMo's estimates for excess mortality be corrected from the effect of death reporting lags by using polynomial regressions. This holds for the flows in each date and their cumulative sum, as well as national and regional data. These adjustments can be applied by surveillance systems in other countries.
On the Kinetic Mechanisms of the Reduction and Oxidation Reactions of Iron Oxide/Iron Pellets for a Hydrogen Storage Process
Authors: Gamisch Bernd , Huber Lea , Gaderer Matthias , Dawoud Belal 
Published: November 2022

This work aims at investigating the kinetic mechanisms of the reduction/oxidation (redox) reactions of iron oxide/iron pellets under different operating conditions. The reaction principle is the basis of a thermochemical hydrogen storage system. To simulate the charging phase, a single pellet consisting of iron oxide (90% Fe2O3, 10% stabilising cement) is reduced with different hydrogen (H2) concentrations at temperatures between 600 and 800 °C. The discharge phase is initiated by the oxidation of the previously reduced pellet by water vapour (H2O) at different concentrations in the same temperature range. In both reactions, nitrogen (N2) is used as a carrier gas. The redox reactions have been experimentally measured in a thermogravimetric analyser (TGA) at a flow rate of 250 mL/min. An extensive literature review has been conducted on the existing reactions’ kinetic mechanisms along with their applicability to describe the obtained results. It turned out that the measured kinetic results can be excellently described with the so-called shrinking core model. Using the geometrical contracting sphere reaction mechanism model, the concentration- and temperature-dependent reduction and oxidation rates can be reproduced with a maximum deviation of less than 5%. In contrast to the reduction process, the temperature has a smaller effect on the oxidation reaction kinetics, which is attributed to 71% less activation energy (Ea,Re=56.9 kJ/mol versus Ea,Ox=16.0 kJ/mol). The concentration of the reacting gas showed, however, an opposite trend: namely, to have an almost twofold impact on the oxidation reaction rate constant compared to the reduction rate constant.
Insect Gut Bacteria Promoting the Growth of Tomato Plants (Solanum lycopersicum L.)
Authors: Krawczyk Krzysztof , Szabelska-Beręsewicz Alicja , Przemieniecki Sebastian Wojciech , Szymańczyk Mateusz , Obrępalska-Stęplowska Aleksandra 
Published: November 2022

We investigated gut bacteria from three insect species for the presence of plant growth properties (PGP). Out of 146 bacterial strains obtained from 20 adult specimens of Scolytidae sp., 50 specimens of Oulema melanopus, and 150 specimens of Diabrotica virgifera, we selected 11 strains displaying the following: PGP, phosphate solubility, production of cellulase, siderophore, lipase, protease, and hydrogen cyanide. The strains were tested for growth promotion ability on tomato (Lycopersicon esculentum) plants. Each strain was tested individually, and all strains were tested together as a bacterial consortium. Tomato fruit yield was compared with the negative control. The plants treated with bacterial consortium showed a significant increase in fruit yield, in both number of fruits (+41%) and weight of fruits (+44%). The second highest yield was obtained for treatment with Serratia liquefaciens Dv032 strain, where the number and weight of yielded fruits increased by 35% and 30%, respectively. All selected 11 strains were obtained from Western Corn Rootworm (WCR), Diabrotica virgifera. The consortium comprised: Ewingella americana, Lactococcus garvieae, L. lactis, Pseudomonas putida, Serratia liquefaciens, and S. plymuthica. To our knowledge, this is the first successful application of D. virgifera gut bacteria for tomato plant growth stimulation that has been described.
Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm Varestrongylus eleguneniensis (Strongylida: Protostrongylidae)
Authors: Xie Yue , Chen Yijun , Wang Lidan , Wang Zhao , Zhu Pengchen , Hu Zun , Gu Xiaobin , He Ran , Xu Jing , Jing Bo , Peng Xuerong , Yang Guangyou , Zhou Xuan 
Published: November 2022

The takin lungworm Varestrongylus eleguneniensis (Strongylida: Protostrongylidae) causes lethal bronchopneumonia and represents severe threats to captive and wild populations. However, until now there has been very limited information available concerning the molecular epidemiology and evolutionary biology of V. eleguneniensis. Mitochondrial genomes (mtDNAs) can provide resources for investigations in these areas and, therefore, can assist with the surveillance and control of this lungworm. Herein, the complete mtDNA of V. eleguneniensis was sequenced and characterized with Illumina pipeline analyses. This circular genome (13,625 bp) encoded twelve protein-coding genes (PCGs), two rRNAs, and twenty-two tRNAs, with notable levels of AT and GC skews. Comparative genomics revealed a purifying selection among PCGs, with cox1 and nad6 having the lowest and the highest evolutionary rate, respectively. Genome-wide phylogenies showed a close relationship between V. eleguneniensis and Protostrongylus rufescens in Strongylida. Single gene (PCGs or rRNAs)-based phylogenies indicated that cox1 and nad5 genes shared the same family-level topology with that inferred from genomic datasets, suggesting that both genes could be suitable genetic markers for evolutionary and phylogenetic studies of Strongylida species. This was the first mtDNA of any member of the genus Varestrongylus, and its comprehensive molecular characterization represents a new resource for systematic, population genetic and evolutionary biological studies of Varestrongylus lungworms in wildlife.
De Novo Transcriptome Assembly and Analysis of Longevity Genes Using Subterranean Termite (Reticulitermes chinensis) Castes
Authors: Haroon  , Li Yu-Xin , Ye Chen-Xu , Su Jian , Nabi Ghulam , Su Xiao-Hong , Xing Lian-Xi 
Published: November 2022

The longevity phenomenon is entirely controlled by the insulin signaling pathway (IIS-pathway). Both vertebrates and invertebrates have IIS-pathways that are comparable to one another, though no one has previously described de novo transcriptome assembly of IIS-pathway-associated genes in termites. In this research, we analyzed the transcriptomes of both reproductive (primary kings “PK” and queens “PQ”, secondary worker reproductive kings “SWRK” and queens “SWRQ”) and non-reproductive (male “WM” and female “WF” workers) castes of the subterranean termite Reticulitermes chinensis. The goal was to identify the genes responsible for longevity in the reproductive and non-reproductive castes. Through transcriptome analysis, we annotated 103,589,264 sequence reads and 184,436 (7G) unigenes were assembled, GC performance was measured at 43.02%, and 64,046 sequences were reported as CDs sequences. Of which 35 IIS-pathway-associated genes were identified, among 35 genes, we focused on the phosphoinositide-dependent kinase-1 (Pdk1), protein kinase B2 (akt2-a), tuberous sclerosis-2 (Tsc2), mammalian target of rapamycin (mTOR), eukaryotic translation initiation factor 4E (EIF4E) and ribosomal protein S6 (RPS6) genes. Previously these genes (Pdk1, akt2-a, mTOR, EIF4E, and RPS6) were investigated in various organisms, that regulate physiological effects, growth factors, protein translation, cell survival, proliferation, protein synthesis, cell metabolism and survival, autophagy, fecundity rate, egg size, and follicle number, although the critical reason for longevity is still unclear in the termite castes. However, based on transcriptome profiling, the IIS-pathway-associated genes could prolong the reproductive caste lifespan and health span. Therefore, the transcriptomic shreds of evidence related to IIS-pathway genes provide new insights into the maintenance and relationships between biomolecular homeostasis and remarkable longevity. Finally, we propose a strategy for future research to decrypt the hidden costs associated with termite aging in reproductive and non-reproductive castes.
Synthesis of an Anti-CD7 Recombinant Immunotoxin Based on PE24 in CHO and E. coli Cell-Free Systems
Authors: Krebs Simon K. , Stech Marlitt , Jorde Felix , Rakotoarinoro Nathanaël , Ramm Franziska , Marinoff Sophie , Bahrke Sven , Danielczyk Antje , Wüstenhagen Doreen A. , Kubick Stefan 
Published: November 2022

Recombinant immunotoxins (RITs) are an effective class of agents for targeted therapy in cancer treatment. In this article, we demonstrate the straight-forward production and testing of an anti-CD7 RIT based on PE24 in a prokaryotic and a eukaryotic cell-free system. The prokaryotic cell-free system was derived from Escherichia coli BL21 StarTM (DE3) cells transformed with a plasmid encoding the chaperones groEL/groES. The eukaryotic cell-free system was prepared from Chinese hamster ovary (CHO) cells that leave intact endoplasmic reticulum-derived microsomes in the cell-free reaction mix from which the RIT was extracted. The investigated RIT was built by fusing an anti-CD7 single-chain variable fragment (scFv) with the toxin domain PE24, a shortened variant of Pseudomonas Exotoxin A. The RIT was produced in both cell-free systems and tested for antigen binding against CD7 and cell killing on CD7-positive Jurkat, HSB-2, and ALL-SIL cells. CD7-positive cells were effectively killed by the anti-CD7 scFv-PE24 RIT with an IC50 value of 15 pM to 40 pM for CHO and 42 pM to 156 pM for E. coli cell-free-produced RIT. CD7-negative Raji cells were unaffected by the RIT. Toxin and antibody domain alone did not show cytotoxic effects on either CD7-positive or CD7-negative cells. To our knowledge, this report describes the production of an active RIT in E. coli and CHO cell-free systems for the first time. We provide the proof-of-concept that cell-free protein synthesis allows for on-demand testing of antibody–toxin conjugate activity in a time-efficient workflow without cell lysis or purification required.
Comparison of Growth and Chemical Profile of Diatom Skeletonema grevillei in Bioreactor and Incubation-Shaking Cabinet in Two Growth Phases
Authors: Frleta Roberta , Popović Marijana , Smital Tvrtko , Šimat Vida 
Published: November 2022

Marine microalgae, diatoms, are considered a source of a wide range of high-value compounds, and numerous studies indicate their biotechnological potential in the food and feed industry, cosmetic industry, nanotechnology, pharmaceutical industry, biodiesel production, fertilizers, and wastewater treatment. The aim of this study was to compare the growth, chemical profiles, and antioxidant activity of the diatom Skeletonema grevillei cultivated in a bioreactor and an incubation-shaking cabinet at different growth phases (after 192 and 312 h). Growth was monitored by evaluating cell density with the Sedgewick Rafter chamber, and the collected biomass was extracted with 70% ethanol assisted by ultrasound. Extracts were evaporated to dryness and compounds were identified in derivatized form by gas chromatography and mass spectrometry (GC-MS) analysis, while antioxidant capacity was evaluated by DPPH and ORAC. Significantly faster growth was observed in the bioreactor than in the incubation-shaking cabinet. Oleamide, palmitelaidic acid, glycerol monostearate, myristic acid, cholesterol, eicosapentaenoic acid, 1-monopalmitin, and 24-methylene cholesterol were identified as the major compounds in both systems. Among them, oleamide was the dominant compound in both systems. It is also shown that prolonging the cultivation period had a direct effect on increasing the extract yield. The highest DPPH inhibition (11.4 ± 1%) and ORAC values (93.3 ± 8.4 mM TE) were obtained for the S. grevillei extract recovered from the bioreactor after 312 h. The obtained results contribute to the possibility of using S. grevillei for various biotechnological applications in the future.
Effect of Dimpled Rib with Arc Pattern on Hydrothermal Characteristics of Al2O3-H2O Nanofluid Flow in a Square Duct
Authors: Kumar Anil , Maithani Rajesh , Sharma Sachin , Kumar Sunil , Sharifpur Mohsen , Alam Tabish , Gupta Naveen Kumar , Eldin Sayed M. 
Published: November 2022

The present work is concerned with the experimental analysis of the thermal and hydraulic performance of Al2O3−H2O nanofluid flow in dimpled rib with arc pattern in a square duct. The Alumina nanofluid consists of nanoparticles having a size of 30 nm. Reynolds number Renum studied in the square duct range from 5000 to 26,000. The nanoparticle volume fraction (ϕnp) ranges from 1.5% to 4.5%, the ratio of dimpled-arc-rib-height to print-diameter HAD/Pd ranges from 0.533 to 1.133, the ratio of the dimpled-rib-pitch to rib height PAD/HAD range from 3.71 to 6.71 and dimpled arc angle (αAD) range from 35° to 65°. The Al2O3−H2O-based nanofluid flow values of Nusselt number Nurs and friction factor frs are higher in comparison to pure water. The dimpled ribs in the arc pattern significantly improved the thermal-hydraulic performance of the investigated test section. The nanoparticle concentration of 4.5%, the ratio of dimpled arc rib height to print diameter of 0.933, the relative dimpled arc rib height of 4.64 and the dimpled arc angle of 55° deliver the maximum magnitude of the heat transfer rate. The maximum value of the thermal-hydraulic performance parameters was found to be 1.23 for Al2O3−H2O-based nanofluid flow in a dimpled rib with arc pattern square duct for the range of parameters investigated. Correlations of Nurs, frs and ηrs have been developed for the selected range of operating and geometric parameters.
Analyse des domaines lexicaux et sémantico-conceptuels structurant le discours des cosmétiques antiâge en anglais
Authors: Bordet Lucile , Jamet Denis 
Published: November 2022

Se fondant sur un corpus de 60 publicités en anglais de cosmétiques antiâge destinés à un public féminin, cet article propose dans un premier temps une analyse textométrique du corpus, en termes de fréquences lexicales et de concordances privilégiées. Il ressort de cette analyse que les lexèmes les plus fréquents ne renvoient pas tant, de façon assez logique, aux notions mêmes de vieillesse et vieillissement qu’à une partie du corps (skin) et aux effets (wrinkles ; lines ; spots) que le temps ((anti-)aging ; age ; younger ; week) a sur elle. L’article propose ensuite une analyse des domaines sémantico-conceptuels majoritairement représentés dans le corpus, par le truchement des métaphores conceptuelles qui structurent ces représentations, puis termine sur une analyse de la conceptualisation de l’âge et du temps qui passe tels qu’ils sont représentés dans les publicités pour cosmétiques antiâge vendus aux femmes, confirmant l’analyse textométrique menée précédemment. L’axiologie du temps est finalement abordée par le biais de la prosodie sémantique.
Communication territoriale à l’anglaise : analyse d’une représentation sociale
Authors: Cassel-Piccot Muriel 
Published: November 2022

Cette étude se situe au carrefour des champs de la civilisation britannique et de la communication de masse et à la croisée des analyses de contenu et du discours. Elle se propose d’examiner les campagnes de promotion du Royaume-Uni menées par l’organisme VisitBritain entre 2012 et 2019. Après une présentation du contexte politique, économique, social et culturel de la période considérée et une exposition de l’identité comme représentation collective, l’analyse qui porte sur un corpus de 305 publicités fixes décrypte l’image du pays promu, les valeurs retenues pour le caractériser et l’idéologie véhiculée. Les résultats font apparaître une nation unie, non fragmentée, prospère, riche de diversités, un territoire qui importe et exporte l’excellence, une puissance mondiale de premier rang, un microcosme national du macrocosme mondial, en somme des visions qui ne sont que partiellement en adéquation avec la réalité mais qui répondent à des enjeux politiques. À travers ces publicités, la britannicité se retrouve tout à la fois édulcorée et redynamisée : les valeurs chères à la plus vieille démocratie du monde sont bien illustrées mais ses spécificités socioculturelles se diluent dans une vision mondialisante et uniformisante du pays.
(In)achèvement de la poésie – Sur Ivar Ch’Vavar
Authors: Auclerc Benoît 
Published: November 2022

Ivar Ch’Vavar (né en 1951) déclare à maintes reprises l’histoire de la poésie « achevée » depuis la fin du xixe siècle, avec Rimbaud, Lautréamont et Mallarmé. Continuer à écrire de la poésie relève dès lors de l’impossible, et constitue une entreprise menacée par le ressassement mélancolique, l’imposture, ou l’inachèvement. Pour autant, qu’il s’agisse d’inventer une poésie picarde, celle de la « Grande Picardie mentale », de compiler la « poésie des fous et des crétins dans le nord de la France » (Cadavre grand m’a raconté), quitte pour cela à recourir à de multiples hétéronymes, ou de se confronter à l’épopée (Titre), la poésie d’Ivar Ch’Vavar ne se détourne pas de sa tâche historique, mais elle le fait en multipliant les légendes, les mystifications et les dérapages burlesques. Constatant le caractère inaccessible de l’idée de poésie, Ch’Vavar produit une poésie historique malgré elle, où ce qui signe l’échec de la poésie est aussi ce qui en permet la relance.
Poésie, photographie et contre-histoires : identités en tension
Authors: Nachtergael Magali 
Published: November 2022

Autour du travail poétique, photographique et performé de Lisette Lombé (1978, Belgique), il s’agit d’analyser la politique du document photographique dans des œuvres personnelles, militantes et à dimension historique. Après un rappel sur des œuvres emblématiques et un état des lieux de la création contemporaine, l’article se concentre sur Black Words (L’Arbre à Paroles, 2018), recueil phototextuel en français de la poétesse belgo-congolaise dérivé d’une série de performances au cœur desquelles l’histoire coloniale et la prise de parole sont centrales. L’ouvrage est mis en perspective avec des productions phototextuelles anglophones, témoignant de l’Archival Turn dans l’art contemporain et d’une poétique visuelle engagée liée aux identités noires, qu’elles soient liées à la situation post-ségrégationniste états-unienne ou postcoloniale européenne.
Illustrer L’Année terrible
Authors: Page Camille 
Published: November 2022

Dans L’Année terrible, Victor Hugo narre avec émotion les déboires d’une société profondément marquée par la débâcle de la guerre franco-prussienne ainsi que par l’épisode traumatique de la Commune. Peu de temps après la parution du recueil, les éditeurs s’emparent du verbe poétique pour lui adjoindre une iconographie riche de sens. À travers l’étude comparative de trois éditions illustrées qui se succèdent chronologiquement – celle de Michel Lévy en 1874, celle d’Eugène Hugues en 1879 et celle de l’Édition Nationale en 1888 –, l’article se propose d’étudier la manière dont s’articule en cette fin de xixe siècle le fragile équilibre entre poésie, histoire et illustration. En mettant en lumière les partis-pris iconographiques qui prévalent au fil des publications, il pose ainsi la question de la réception du texte hugolien et de ses variations relatives au contexte de publication. Entre souvenir bruyant de la défaite humiliante contre la Prusse et silence autour de la révolution parisienne et du sort des communards, l’image reflète en un sens les brèches d’une société profondément meurtrie par l’événement historique.
Captures, traces, empreintes poétiques de l’expérience historique : Paris, xixe siècle ; ghetto de Varsovie, 1941-1943
Authors: Lyon-Caen Judith 
Published: November 2022

Cet article propose un parcours entre deux massifs poétiques éloignés : la poésie baudelairienne et des poèmes écrits dans le ghetto de Varsovie entre 1941 et 1943. En réfléchissant sur la question des « circonstances » en poésie et en utilisant les réflexions d’Yves Bonnefoy sur l’« événement poétique », on se demande ici ce que documentent ces poèmes en tant que poèmes, en les observant comme autant d’actes d’écriture effectués dans des formes conçues, identifiées et transmises comme relevant de « la poésie » en leur temps comme dans le nôtre. Il s’agit ainsi de dessiner quelques pistes pour une lecture « historienne » de la poésie, une lecture sans réduction documentaire, attentive à la création poétique comme pratique sociale et au geste poétique comme geste dans l’histoire.