bluuebunny/crossref_metadata_2025_split huggingface dataset:
Speed breeding to accelerate crop improvement
Authors: Temesgen Begna 
Published: June 2022

Global food security has become a major issue as the human population grows and the environment changes, with the current rate of improvement of several important crops inadequate to meet future demand. Crop plants have extended generation times, which contributes to the slow rate of progress. However, speed breeding has revolutionized the entire world by reducing generation time and speeding up breeding and research programs to improve crop varieties. In the absence of an integrated pre-breeding program, breeding new and high-performing cultivars with market-preferred traits can take more than ten years. After the first cross with parental genotypes, a large amount of time, space, and resources are committed to the selection and genetic advancement stages during the early stages of breeding. Speed breeding has the ability to shorten the time it takes to develop, market, and commercialize cultivars. Crop improvement in the face of a fast-changing environment and an ever-increasing human population is a major concern for scientists around the world. Current crop enhancement projects are progressing at a rate that is insufficient to meet food demand. Crop redesign is urgently needed for climate resilience, as well as long-term yield and nutrition. Crop progress is slowed significantly by the long generation time required by crop plants during the breeding process. Speed breeding is now being used on a large scale to shorten generation time and support multiple crop generations per year as a solution in this approach. Researchers are now using an integrated approach to improve breeding efficiency, combining speed breeding with current plant breeding and genetic engineering methods. Speed breeding is a promising approach for achieving nutritional security and sustainable agriculture by shortening breeding cycles for food and industrial crop enhancement. Speed breeding is a methodology that allows plant breeders to improve crop production by adjusting temperature, light duration, and intensity to boost plant development. It uses an artificial source of light, which is kept on continuously, to activate the photosynthetic process, which leads to growth and reproduction much earlier than normal. This will assist in meeting the demands of the future’s rising population. This can be accomplished using a variety of technologies, including genotyping, marker-assisted selection, high throughput phenotyping; gene editing, genomic selection, and re-domestication, all of which can be combined with speed breeding to allow plant breeders to keep up with a changing climate and growing human population.
Levels of apoptosis factors in mitochondria of brain cortex cells in female С57ВL/6 mice in dynamics of B16/F10 melanoma growth combined with comorbidity
Authors: Frantsiyants E. M. , Neskubina I. V. , Cheryarina N. D. , Surikova E. I. , Shikhlyarova A. I. , Bandovkina V. A. , Nemashkalova L. A. , Kaplieva I. V. , Trepitaki L. K. , Kachesova P. S. 
Published: June 2022

Purpose of the study. To analyze the apoptosis indicators in mitochondria of brain cortex cells in female С57ВL/6 mice in the dynamics of B16/F10 melanoma growth alone and in combination with comorbidity, i.e. chronic neurogenic pain.Materials and methods. Female С57ВL/6 mice (n = 168) were used in the experiment. Groups accounted: intact group (n = 21); control group (n = 21) with a model of chronic neurogenic pain (CNP); comparison group (n = 63) with B16/F10 melanoma transplanted subcutaneously; main group (CNP + B16/F10) (n = 63). Levels of cytochrome C (ng/mg protein), caspase 9 (ng/mg protein), Bcl‑2 (ng/mg protein), AIF (ng/mg protein), calcium (Ca 2+) (mMol/g protein) were measured by ELISA in mitochondrial samples. Statistical analysis was performed using the Statistica 10.0 program.Results. In a week of the tumor growth in presence of comorbidity, i.e. CNP, levels of calcium in murine brain cortex mitochondria were 1.4 times higher (p < 0.05) than in the comparison group at the same time; in 2 weeks the levels declined by 80.1 times and after 3 weeks declined by 37.7 times. Compared to the values in the comparison group AIF levels in animals with CNP+B16/F10 were lower by 25 and 1.8 times (p < 0.05) at weeks 1 and 3, respectively. Higher levels of Вcl‑2 in the group with CNP + B16/F10 were registered at weeks 2 and 3 by 2 and 1.4 times (p < 0.05), respectively. Levels of cytochrome C were decreased in animals with CNP+B16/F10 at weeks 1–3 by 3.2, 1.5 (p < 0.05) and 2.8 times, respectively. Caspase 9 in CNP+B16/F10 after 3 weeks exceeded the values in the comparison group by 2.6 times.Conclusions. Combination of CNP and melanoma at an early stage in the animal body promotes the accumulation of calcium and suppression of AIF and cytochrome C in mitochondria of the brain cortex. By the terminal stage of tumor growth in presence of comorbidity (CNP), suppression of most units of the respiratory chain of mitochondria of brain cortex cells is formed.
Analysis of regulatory documents regulating the fight against COVID-19 and other healthcare tools used to manage the COVID-19 pandemic in Kazakhstan
Authors: Benberin V. V. , Kaikenov B. T. , Samsonov Yu. V. 
Published: June 2022

Purpose of the study. Explore organizational tools for managing health system resources to fight the COVID‑19 pandemic in Kazakhstan.Materials and methods. The analysis of regulatory documents (5 decrees of the chief sanitary doctors and 2 orders of the Ministry of Health of the Republic of Kazakhstan) regulating measures for the COVID‑19 pandemic was carried out. These legal acts were approved in the first months after the declaration of the state of emergency in the country after the detection of the first cases of coronavirus infection in Kazakhstan. These laws and regulations governed the provisions and measures to combat COVID‑19. And also the analysis of measures for the introduction of quarantine or restrictions based on the monitoring of the R number of the virus was carried out.Results. The article discusses the issues of resource provision to contain the spread of COVID‑19 in the country. The analysis of the regulatory legal acts regarding the types of assistance provided to the population to prevent the spread of infection, as well as the treatment strategy for patients suffering coronavirus infection. These laws regulate the introduction, weakening/strengthening or cancellation of restrictive measures in the regions, mainly related to the activities of organizations and public places where there is a high concentration of the population, as well as the movement of masses of people.Conclusion. The study showed what types of assistance are provided to the population to halt the COVID‑19 pandemic, ranging from tactics at the outpatient level to the deployment of care at the inpatient level. In addition, the monitoring of the R value of the virus can be a working tool for the authorities in the introduction of quarantine measures.
Impact of Water Supplementation With Turmeric and Tamarind Extracts on Broiler Liver Performance
Authors: Sudatri N.W. , Suartini N.M. , Dewi G.A.M. Kristina , Mahardika I G. , Bidura I G.N. Gde 
Published: June 2022

Turmeric and tamarind are medicinal plants that have a variety of health benefits, including laxative, antibacterial, anticancer, and hepatoprotective properties. The focus of this research was to look at the histology of the liver and the levels of SGPT-SGOT in broilers fed turmeric-tamarind extracts water at a rate of 2%. This study used a completely randomized design, with treatment options including a control, a mixture of turmeric rhizome and tamarind fruit (2%), and concentrations of 2% turmeric rhizome and 2% tamarind. The turmeric rhizome and tamarind extracts treatment was given in drinking water ad libitum. Quantitative data from the study were analyzed statistically, and if there was a significant effect, Duncan’s test was used; qualitative data were presented with descriptions and images. The blood levels of SGPT in the control broilers were higher than in the broilers exposed to any of the other treatments. Also, in the control broiler livers, hydrofic degeneration, nucleus pyknosis, and congestion were higher than in the treatment group broilers, but these were still within normal limits. Water supplementation with a mixture of turmeric rhizome and tamarind fruit extracts did not have a negative effect on the performance of the broiler livers.
Keywords: turmeric, tamarind, liver performance, broiler
Effectivity of karamunting Melastoma malabathricum leaves in inhibiting ovarian development of Nile tilapia Oreochromis niloticus
Authors: Yogo Tri saloko  , Muhammad Agus Suprayudi  , Muhammad Zairin Jr.  
Published: June 2022

ABSTRACT

Tilapia gonads mature quickly before reaching market size, caused by a diverting of feed energy from growth to reproduction. As a result, somatic growth is disrupted to achieve market size, the operational costs are high, and the rearing period is longer. This study aims to evaluate the ability of karamunting leaf extracts to inhibit the development of tilapia gonads. This study used a complete randomized design with four treatments and three replications, namely 0 mg/kg, 25 mg/kg, 50 mg/kg, and 100 mg/kg of karamunting leaf extract. Tilapia fish weighed 13–14 g were kept in an aquarium measuring 100×60×50 cm with a stocking density of 20 fish/ aquarium. Fish were fed twice a day at 8 a.m and 5 p.m in at satiation. Sampling was carried out at the beginning of the study, on day 30th and day 60th. On day 30th the result showed that the best dose in inhibiting the development of fish gonad was 100 mg/kg of karamunting leaf extract that was 1.15 ± 0.19% and the daily growth rate was increased at 2.22 ± 0.06%. On day 60th, the best dose in inhibiting gonad development was 25 mg/kg of karamunting leaf extract, which was 2.49 ± 1.24% and the daily growth rate was increased amount 3.26 ± 0.06%.
Keywords: extract, karamunting leaves, gonad development, tilapia

ABSTRAK

Gonad ikan nila cepat berkembang sebelum mencapai ukuran pasar, menyebabkan pengalihan energi pakan dari pertumbuhan ke reproduksi. Akibatnya, pertumbuhan somatik terganggu sehingga untuk mencapai ukuran pasar biaya operasional tinggi dan masa pemeliharaan lebih lama. Penelitian ini bertujuan untuk mengevaluasi ekstrak daun karamunting dalam menghambat perkembangan gonad ikan nila. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan dan tiga ulangan; yaitu 0 mg/kg, 25 mg/kg, 50 mg/kg, dan 100 mg/kg ekstrak daun karamunting. Ikan nila berukuran 13–14 g dipelihara di akuarium berukuran 100×60×50 cm dengan padat tebar 20 ikan/akuarium. Ikan diberi makan dua kali sehari pada jam 8 pagi dan 5 sore dengan at satiation. Pengambilan sampel dilakukan pada awal penelitian, hari ke-30 dan hari ke-60. Hasil pada hari ke-30 menunjukan bahwa dosis terbaik dalam menghambat perkembangan gonad pada ikan uji adalah 100 mg/kg ekstrak daun karamunting yaitu 1.15 ± 0.19% dan meningkatkan pertumbuhan harian 2.22 ± 0.06%. Pada hari ke-60 dosis terbaik dalam menghambat perkembangan gonad adalah 25 mg/kg ekstrak daun karamunting yaitu 2.49 ± 1.24% dan meningkatkan pertumbuhan harian 3.26 ± 0.06%.
Kata kunci: daun karamunting, ekstrak, ikan nila, pertumbuhan gonad
TINGKAT KESUKAAN DAN NILAI GIZI COOKIES DENGAN PENAMBAHAN TEPUNG DAUN KATUK DAN TEPUNG DAUN KELOR UNTUK IBU MENYUSUI
Authors: Nu'man Thobagus Muhammad , Bahar Asrul 
Published: June 2022

Katuk leaves [Sauropus androgynus (L.) Merr] and moringa leaves (Moringa oleifera Lam.) are plants that can be used as galactagogues. Galactagogues compounds in both can stimulate the hormones prolactin and oxytocin, thereby increasing milk production. Katuk leaves and moringa leaves have a natural taste that is not favoured so that processing and formulation of other ingredients are needed to make the two ingredients more preferable. With an average growth from 2016-2020 of 4.250%, cookies can be a medium to help meet human needs. This study purposed to determine the level of preference and nutritional value of cookies with the addition of katuk leaves and moringa leaves as a snack for nursing mothers. This study used a factorial research design with 2 factors, namely 3 levels of katuk leaf flour (5 g, 10 g, 15 g) and 2 levels of moringa leaf flour (5 g, 10 g). The hedonic test data were processed using the Friedman test and further test with the Wilcoxon signed-rank test. Calculations used to test the effectiveness to determine the selected formula showed that the F4 treatment (5 g katuk leaf flour: 10 g moringa leaf flour) had the highest effectiveness value of 0.95. The results of the chemical test on the F4 treatment had a water content of 1.02%, carbohydrates 76.88%, protein 12.05%, fat 7.85%, and energy contributions of 423.50 kcal per 100 g, and 105.87 kcal per dose serving (30 g).
Keywords: breastfeeding mothers, cookies, katuk leaves, moringa leaves
OSTEOPOROSIS SCREENING TECHNOLOGY APPLICATION TO IMPROVE HEALTHY LIFESTYLES OF SCHOOL RESIDENTS IN PORONG, SIDOARJO
Authors: Muqmiroh Lailatul , Kartika Sari Amillia , Devianti Putri Berliana , Muhaimin Muhaimin 
Published: June 2022

Background : Osteoporosis is one of degenerative disease that can affect elderly and children as well. Knowledge about Osteoporosis has not been fully understood by school residents and their community. Objectives : This activity aims to (1) increase the school residents knowledge about Osteoporosis, (2) provide digital health communication media that can be used widely and easily by school residents, (3) increase the ability of school residents to use osteoporosis screening technology application and disseminate to the community. Method : Training and mentoring using ATOs-A application media and pocket books. Monitoring and evaluation is carried out to see and assist teachers in using the Osteoporosis screening application. Knowledge improvement is measured by pre-post test using questionnaire as a tool. Result : There is improvement of school residents knowledge about Osteoporosis and how to use screening technology application. So that, they have more encouragement to improve their knowledge and propagate it to community. Conclusion : Community service has a positive response from school residents and benefits to improve the healthy lifestyle in that area.
THE RELATIONSHIP BETWEEN NURSES’ KNOWLEDGE AND COMPLIANCE IN PERFORMING FALL RISK RE-ASSESSMENT
Authors: Sulistyowati Arlina Dhian , Rusminingsih Esri , Sari Devi Permata , Kayati Fitriana Noor , A Yuli Widhi 
Published: June 2022

Introduction: The fall incidence will have a detrimental impact on the hospital, staffs and patients as the service recipients. This can be prevented by conducting fall risk reassessment. It is an assessment of patient’s fall risk carried out by nurses. This study aims to determine the level of knowledge and compliance of nurses in carrying out a patient fall risk reassessment in the inpatient ward. Methods: This study used an observational analytical design with a quantitative method and a cross-sectional approach. A total sampling of 48 respondents were used as samples. Kendall Tau-b test was employed as the analysis technique. Results: The respondents were mostly female or as many as 33 people (68.8%). The educational level of the respondents was mostly D-III or as many as 32 people (66.7%). The majority of respondents’ working period was 3-5 years or as many as 26 people (54.2 %). The average age of the respondents was 27.5 years, the respondents' knowledge was mostly good or as many as 24 people (50%), and the respondents’ compliance in carrying out the fall risk reassessment was mostly compliant or as many as 41 people (85.4%). This study utilized the Kendall Tau-b test and obtained p value = 0.006 and Correlation Coefficient of 0.387. Conclusions: There is a significant relationship between nurses' knowledge of fall risk and their compliance in carrying out a fall risk reassessment in the inpatient ward of the PDHI Yogyakarta Islamic Hospital. The higher the knowledge, the higher the level of nurse compliance.
Effective Management of Non-Surgical Periodontal Treatment in a Patient with Severe Gingival Enlargement and Periodontal Bone Loss: A Case Report
Authors: Dalipi Zana S , Dragidella Fatmir , Dragidella Donika K 
Published: June 2022

The immune-inflammatory response of periodontal tissue can be influenced by various systemic variables such as hormone disorders, hematologic diseases, nutrition, and medications. Inflammatory cytokines released by infected periodontal tissue are implicated in suppressing erythrocyte growth, differentiation, and erythropoiesis inhibition.	We present a case of non-surgical periodontal treatment in a 31-year-old female patient with severe gingival enlargement associated with aggressive bone destruction combined with iron deficiency anemia (IDA). The periodontal treatment plan was achieved in collaboration with the hematologist, starting with oral hygiene instruction, scaling and root planning (SRP) by sextants with systemic antibiotic therapy combined with a chemical agent and low-level-laser-therapy (LLLT), and extraction of hopeless teeth.	Effective non-surgical periodontal treatment with SRP and LLLT reduces inflammation and gingival enlargements and controls the recolonization of subgingival sites by periodontal pathogens as a crucial factor to proceed with periodontal surgery. Function, aesthetics, and the patient’s social communication are all improved.
Oxidative Stress Intensity in Children and Adolescents with a New Coronavirus Infection
Authors: Rychkova Lyubov V , Darenskaya Marina A , Semenova Natalya V , Kolesnikov Sergey I , Petrova Alla G , Nikitina Olga A , Brichagina Anastasia S , Kolesnikova Lyubov I 
Published: June 2022

The aim of our research was to assess the intensity of oxidative stress (OS) in children and adolescents with COVID-19 using the oxidative stress index (OSI). Methods and Results: The study was conducted between May 2020 and March 2021. The main group included 17 children and adolescents [8(47.1%) boys and 9(52.9%) girls; mean age of 12.35±4.01 years] with diagnosed COVID-19 infection (mild to moderate course) selected as a result of the primary diagnostic examination from among those admitted to hospitalization at the Irkutsk Regional Infectious Diseases Clinical Hospital. The control group included 17 healthy children and adolescents (average age of 12.35±4.01 years) matched by copy-pair type.	The obtained data indicated statistically significant differences in a number of parameters between children and adolescents with COVID-19 and the control groups. We found statistically significant higher levels of LPO products (CDs, P<0.0001; KD and CT, P=0.006; and TBARs, P=0.013) in the study group than in the control group. Among AOD system parameters, the levels of retinol (P=0.015) and reduced glutathione (P=0.048) and SOD activity (P<0.0001) were statistically lower in the study group than in the control group. The OSI level was significantly greater (by 8.5 times, P=0.028) in the study group than in the control group, which confirms the development of antioxidant deficiency in COVID-19. Conclusion: The results of the assessment of OSI in children and adolescents with COVID-19 indicate insufficient activity of some critical components of AOD and a shift of the redox balance toward pro-oxidant factors, which can have extremely negative consequences in the development of the disease. In this regard, we recommend carrying out corrective measures to stabilize LPO/AOD parameters by including drugs with antioxidant properties in the treatment complex.
Endophytic bacterium Bacillus aryabhattai induces novel transcriptomic changes to stimulate plant growth.
Authors: Xu Hongli , Gao Jingyao , Portieles Roxana , Du Lihua , Gao Xiangyou , Borrás-Hidalgo Orlando 
Published: June 2022

Abstract
In nature, plants interact with a wide range of microorganisms, and most of these microorganisms have the ability to promote plant growth through the induction of important molecular pathways. The current study evaluated whether the endophytic bacterium Bacillus aryabhattai encourages plant growth and the transcriptional changes that might be implicated in this effect. The endophytic bacterium promotes the growth of Arabidopsis and tobacco plants. The transcriptional changes in Arabidopsis plants treated with the bacterium were also identified, and the results showed that various genes, such as cinnamyl alcohol dehydrogenase, apyrase, thioredoxin H8, benzaldehyde dehydrogenase, indoleacetaldoxime dehydratase, berberine bridge enzyme-like and gibberellin-regulated protein, were highly expressed. Also, endophytic bacterial genes, such as arginine decarboxylase, D-hydantoinase, ATP synthase gamma chain and 2-hydroxyhexa-2,4-dienoate hydratase, were activated during the interaction. These findings demonstrate that the expression of novel plant growth-related genes is induced by interaction with the endophytic bacterium B. aryabhattai and that these changes may promote plant growth in sustainable agriculture.
In-vitro Cytotoxicity and anticancer drug release behavior of Methionine-coated magnetite nanoparticles as carriers.
Authors: Yeganeh Faten Eshrati , Yeganeh Amir Eshrati , Fatemizadeh Mahdi , Far Bahareh Farasati Farasati , Quazi Sameer , Safdar Muhammad 
Published: June 2022

Abstract
A novel and specific Drug delivery for in vitro cancer-targeted are developed successfully by a simple one-step method. A CoFe2O4@Methionine core-shell nanoparticle was prepared by the reflux assay which amino acid in the surface makes ferrite biocompatible, enhances its chemical stability, and improves the drug loading capacity. The synthesized nanoparticles were characterized using FTIR, TGA, XRD, SEM, TEM, and VSM which coating amino acid on the surface of CoFe2O4 was confirmed by XRD and TGA. The appearance of a new peak for C≡N confirms the formation of Letrozole loaded carrier in the FTIR. The vibrating sample magnetometer of both bare CoFe2O4 and Methionine-coated CoFe2O4 nanoparticles exhibited room-temperature superparamagnetic behavior with a saturation value of 46emu/g and 16.8emu/g, respectively. The morphology and size of samples were characterized by SEM and TEM that the average size of the particle was around 28–29 nm. The loading of Letrozole and the effect of pH (5, 7.4) on the release behavior of the carrier was studied. The result of the drug release in pH equals 5 was about 88% which higher than pH equals 7.4. Also, the preparation had been evaluated for determining its cytotoxicity using MCF-7, MDA-MB-231, and MCF10A cells as an in vitro model, and the result vitro experiments showed that CoFe2O4@Methionine could significantly reduce cancer in cells model. These results demonstrate that core-shell nanoparticle was prepared is biocompatible and have potential use as drug delivery.