bluuebunny/crossref_metadata_2025_split huggingface dataset:
CLONING AND EXPRESSION OF RECOMBINANT PROTEIN OF SUMO, FUSED WITH BIOTIN ACCEPTOR PEPTIDE
Authors: Kulyyassov A.T. , Ramankulov Ye.M. , Ogryzko V.V. 
Published: March 2022

Post-translation modifications by SUMO are involved in a plethora of biological processes, including cell proliferation. Since SUMOylation is an essential regulatory mechanism in signal transduction, violation of the processes associated with SUMOylation can lead to various diseases, including cancer.
We adopted the proximity utilizing biotinylation method, based on co-expression of recombinant proteins within a single cell: the protein of interest fused with the biotin ligase BirA and its partner with the biotin acceptor peptide (BAP). This co-expression allows for accurate quantitative assessment of the extent of the interaction of various molecules in vivo.
Using this approach, the aim of this work was to construct a new plasmid vector containing a SUMO gene fused with a BAP and to implement its expression in HEK293T cells.
Co-expression of the BAP–SUMO and BirA-HP1g (BirA-HP1a) recombinant proteins in HEK293T cells resulted in protein biotinylation, which are SUMOylated proximal proteins (or interaction partners) of the proteins of interest BirA-HP1g and BirA-HP1a.
Projection-Based Model Reduction for Coupled Conduction—Enclosure Radiation Systems
Authors: Brunini Victor , Parish Eric J. , Tencer John , Rizzi Francesco 
Published: March 2022

Abstract
A projection-based reduced order model (pROM) methodology has been developed for transient heat transfer problems involving coupled conduction and enclosure radiation. The approach was demonstrated on two test problems of varying complexity. The reduced order models demonstrated substantial speedups (up to 185×) relative to the full order model with good accuracy (less than 3% L∞ error). An attractive feature of pROMs is that there is a natural error indicator for the ROM solution: the final residual norm at each time-step of the converged ROM solution. Using example test cases, we discuss how to interpret this error indicator to assess the accuracy of the ROM solution. The approach shows promise for many-query applications, such as uncertainty quantification and optimization. The reduced computational cost of the ROM relative to the full-order model (FOM) can enable the analysis of larger and more complex systems as well as the exploration of larger parameter spaces.
Thermophysical Investigations of Mango Seed Oil as a Novel Cutting Fluid: A Sustainable Approach Toward Waste to Value Addition
Authors: Naik Dungavath Narayana Swamy , Sharma Varun 
Published: March 2022

Abstract
In the present work, a novel cutting fluid has been extracted from mango (Mangifera indica L.) seeds using the soxhlet technique. Cutting fluid from agricultural waste can reduce the shortage of edible oils and eliminate the need to dispose them. Therefore, nonedible oils are economically comparable with edible oils. In order to validate the effectiveness of extracted cutting fluid, thermophysical characterization and tribology tests were performed. The obtained results were compared with edible and nonedible oils, such as sunflower oil (SFO) and mahua seed oil (MaSO), respectively. Friction tests were conducted using a tribometer with pin-on-disk (WC-Co/Ti-3Al-2.5V) at different tribological conditions. The worn surface and wear mechanism of disks were examined using a scanning electron microscope (SEM) associated with energy-dispersive spectroscopy (EDS) for comparing the elemental composition quantitatively. These findings showed that mango seed oil (MSO) exhibited the lowest coefficient of friction (COF) and wear volume followed by MaSO, SFO, and dry, respectively. SEM analysis showed that no plowing was observed; however, small pits and cracks were found on wear track under MSO. Further, the abovementioned oils were used as a cutting fluid in the machining of Ti-3Al-2.5V (grade 9 alloy). In this regard, MSO has shown a similar effect in turning operation with minimum quantity lubrication (MQL) system.
Petri Net Modeling for Disassembly Process Planning
Authors: Xirouchakis Paul , Kiritsis Dimitris 
Published: March 2022

Abstract
A generic Petri-Net model is presented for the modelling of disassembly planning operations. This is of interest for developing decision support systems within the context of planning for recyclability. The proposed modelling methodology could allow the identification and assessment of cost-effective characteristics of disassembly. The proposed approach for the evaluation and the optimisation of the disassembly process for components, part and subassemblies recovery could be used for optimal disassembly sequence generation by considering the precedence constraints between assembly components. The proposed approach is based upon the Petri Net modelling technology already developed at the CAD/CAM laboratory (LICP) at the Swiss Federal Institute of Technology. In this work a generic dynamic model was developed based on Petri Net technology. The Petri Net model (i) represented manufacturing knowledge of the type of precedence constraints (ii) represented dynamically the process planning procedure itself and (iii) encapsulated all possible process planning solutions (process plans). Furthermore, process planning simulation was performed directly on the Petri Net and reachability analysis of the model gave the complete set of solutions (process plans).
Interaction of Process Mechanisms and Environmental Effects in Secondary Finishing
Authors: Bauer D. , Sheng P. 
Published: March 2022

Abstract
This paper presents a modeling framework to enable environmental evaluation of surface finishing processes. The formulation integrates sub-models analyzing energy use, cutting fluid vaporization, cutting fluid aerosol formation, wheel wear, and chip and workpiece fluid carry-off. The overall model can be used to compare and evaluate the energy use and waste stream mass flux for different design and process specifications. This waste stream output mass flux can then serve as input into models predicting the fate, transport, toxicity, and exposure-based risk of the waste streams from this process alone or in conjunction with the waste streams from other processes at the manufacturing facility. A test case is presented to illustrate use of the model.
Probabilistic Analysis of Accidents Involving Pyrophoric Particle Accumulation in a Closed System Containing High Explosives
Authors: Bott Terrence F. , Eisenhawer Stephen W. 
Published: March 2022

Abstract
This paper describes a probabilistic analysis of an unusual safety concern encountered during safety analysis of a closed system containing high explosive (HE). In this application, a pyrophoric material could be generated inside the system by corrosion of system components. This pyrophore may form extremely small particles that could be transported through a complex path to a high explosive component, where they could collect on exposed surfaces as a result of routine handling operations. A potentially serious accident could be initiated if the system is opened for maintenance and oxygen reaches the pyrophoric particles that are resting on the surface of the high explosive. The pyrophoric particles react vigorously in the presence of oxygen and will ignite the high explosive if there is a sufficient concentration of the particles in a localized area. This paper reports on models for estimating the probability of achieving hazardous concentrations of pyrophoric particles on the explosive surface. These probability estimates are based on occupancy likelihood and geometric models. The probability models provide a means for exploring the effects of material behavior, heat transfer, and material transport on the likelihood of achieving unwanted concentrations of pyrophoric particles on the explosive surface.
Explosive Noise and Radioactivity Risk: French Nuclear Tests
Authors: Margulies T. 
Published: March 2022

Abstract
A one-dimensional nonlinear elastic wave model was used to compute sound transmission through the tuff which was coupled to an ocean transmission calculation of pressure versus distance. Sound levels that are greater than those used for marine mammal protection harassment are received hundreds of kilometers away from the source near areas of U.S. interests. These geoacoustic estimates were also compared to poroelastic Biot model predictions. Furthermore, the ionizing radioactivity released to the lagoon has been assessed obtaining levels of concern for those that would swim in the water in the future if the testing is forgotten or ignored.
Fluid Flow-Induced Vibration of Suspended Nonlinear Cables
Authors: Chang W. K. , Pilipchuk V. , Ibrahim R. A. 
Published: March 2022

Abstract
The nonlinear interaction of the first two in-plane modes of a suspended cable with a moving fluid along the plane of the cable is studied. The governing equations of motion for two-mode interaction are derived based on a general continuum model. The interaction causes the modal differential equations of the cable to be non-self-adjoint. As the flow speed increases above a certain critical value, the cable experiences oscillatory motion similar to the flutter of aeroelastic structures. A coordinate transformation in terms of the transverse and stretching motions of the cable is introduced to reduce the two nonlinearly coupled differential equations into a linear ordinary differential equation governing the stretching motion, and a strongly nonlinear differential equation for the transverse motion. For small values of gravity-to-stiffness ratio the dynamics of the cable is examined using a two-time-scale approach. Numerical integration of the modal equations shows that the cable experiences stretching oscillations only when the flow speed exceeds a certain level. Above this level both stretching and transverse motions take place. The influences of system parameters such as gravity-to stiffness ratio, density ratio, and the fluid flow randomness on the response characteristics are also reported.
Identification of Linearised Parameters for Fluidelastic Instability
Authors: Meskell C. , Fitzpatrick J. A. 
Published: March 2022

Abstract
A method for estimating the parameters of a linearized model of the fluidelastic forces in an array of cylinders is described. The behaviour of a tube on a flexible mount in the third row of an otherwise rigid 5 row normal triangular array, with pitch-to-diamter ratio of 1.58, has been investigated. The fluid stiffness and damping over a range of flow velocities has been estimated from experimental data. The results are then used to predict a stability threshold which is in agreement with previously published data for critical velocities. The damping in the flexible mount has been varied and shown not to effect the estimated fluid parameters.
Computation of Vortex Shedding and Radiated Sound for a Circular Cylinder
Authors: Cox Jared S. , Rumsey Christopher L. , Brentner Kenneth S. , Younis Bassam A. 
Published: March 2022

Abstract
The Lighthill acoustic analogy approach combined with Reynolds-averaged Navier Stokes is used to predict the sound generated by unsteady viscous flow past a circular cylinder assuming a correlation length of ten cylinder diameters. The two-dimensional unsteady flow field is computed using two Navier-Stokes codes at a low Mach number over a range of Reynolds numbers from 100 to 5 million. Both laminar flow as well as turbulent flow with a variety of eddy viscosity turbulence models are employed. Mean drag and Strouhal number are examined, and trends similar to experiments are observed. Computing the noise within the Reynolds number regime where transition to turbulence occurs near the separation point is problematic: laminar flow exhibits chaotic behavior and turbulent flow exhibits strong dependence on the turbulence model employed. Comparisons of far-field noise with experiment at a Reynolds number of 90,000, therefore, vary significantly, depending on the turbulence model. At a high Reynolds number outside this regime, three different turbulence models yield self-consistent results.
Turbulence-Induced Tube Vibration in a Parallel Steam-Water Two-Phase Flow
Authors: Kawamura Koji , Yasuo Akira , Inada Fumio 
Published: March 2022

Abstract
This paper is concerned with the fluid force acting on a tube placed in a parallel steam-water two-phase flow. Based on experiments on the tube vibration induced by the turbulence of two-phase flow, the relationships between the hydrodynamic mass, damping ratio, excitation force, and the fluid flow conditions were evaluated. Mass flow rate, pressure, and void fraction were varied in the experiment to measure random vibration of the tube caused by turbulence in the stream. The hydrodynamic mass and damping ratio were derived by analyzing the vibration data. The excitation force was calculated using a transfer function to represent the relationship between the intensity and excitation force of the vibration given by random statistical theory. It was found that the hydrodynamic mass and damping ratio are little affected by the mass flow rate and pressure but are significantly affected by the void fraction and frequency. In contrast, the vibration excitation force is affected by the pressure as well as the effects of void fraction and frequency. The relationships between them were evaluated.
Margins of Uncertainty in the Prediction of Fluidelastic Instability in Heat Exchanger Tube Bundles
Authors: Goyder H. G. D. 
Published: March 2022

Abstract
During the design and operation of a heat exchanger it is necessary to check for the possibility of fluidelastic vibration. The usual approach is to allow for some pessimism so that there is a margin between the prediction and actual occurrence of damaging vibration. This paper deals with some of the factors that control this margin.
Vibration Damping in Multispan Heat Exchanger Tubes
Authors: Taylor C. E. , Pettigrew M. J. , Dickinson T. J. , Currie I. G. , Vidalou P. 
Published: March 2022

Abstract
Heat exchanger tubes can be damaged or fail if subjected to excessive flow-induced vibration, either from fatigue or fretting-wear. Good heat exchanger design requires that the designer understands and accounts for the vibration mechanisms that might occur, such as vortex shedding, turbulent excitation or fluidelastic instability. To incorporate these phenomena into a flow-induced vibration analysis of a heat exchanger requires information about damping.
Damping in multispan heat exchanger tubes largely consists of three components: viscous damping along the tube, and friction and squeeze-film damping at the supports. Unlike viscous damping, squeeze-film damping and friction damping are poorly understood and difficult to measure. In addition, the effect of temperature-dependent fluid viscosity on tube damping has not been verified.
To investigate these problems a single vertical heat exchanger tube with multiple spans was excited by random vibration. Tests were conducted in air and in water at three different temperatures (25, 60 and 90 °C). At room temperature, tests were carried out at five different preloads. Frequency response spectra and resonant peak-fitted damping ratios were calculated for all tests. Energy dissipation rates at the supports and the rate of excitation energy input were also measured.
Results indicate that damping does not change over the range of temperatures tested and friction damping is very dependent on preload.
Flow-Induced Acoustic Resonance With Various Finned Tube Banks
Authors: Nemoto A. , Takakuwa A. , Tsutsui M. 
Published: March 2022

Abstract
Experimental investigations were earned out on flow induced acoustic resonance with tube banks composed of various tubes such as bare, solid fin, serrated fin, and plate-fin tubes.
Tube banks contained in ducts were connected to a silent wind tunnel and installed in an anechoic room. Sound emitted from the tube bank was measured by microphones on the duct wall and at the duct outlet.
The results clarified the effects of the fins on tube banks. In a wide longitudinal pitch of tube bank geometry, there were no great differences in resonant effect, although fins help to emit flow noise. In a narrow geometry, it was revealed that the characteristics of the fin participate in resonance and damping effect. With a complicated geometry, such as serrated fin and plate-fin tubes, there is a greater damping effect and less resonance is generated. In any case, the baffle plates dividing duct width could suppress the resonance.
Identification of Non-Linear Rotor Dynamic Coefficients Using Multiple Circumferential Pressure Measurements
Authors: Spirig Michael , Staubli Thomas 
Published: March 2022

Abstract
A new approach to determine rotor dynamic coefficients based on multiple circumferential pressure measurements is proposed. With the newly introduced procedure, the identification of all, even non-linear, rotor dynamic coefficients can be achieved with one single test run.
The advantage of this procedure is the enormous reduction of test runs and a much simpler and faster identification procedure. A necessary condition for the proposed procedure — besides the simultaneous pressure measurements at multiple positions — is that there are mechanical means to achieve an orbit motion of the rotor (e.g. an eccentric ellipse, Fig. 7.) and that this orbit motion is measured with appropriate accuracy.
Activities in Aeroelasticity at NASA Langley Research Center
Authors: Perry Boyd , Noll Thomas E. 
Published: March 2022

Abstract
This paper presents the results of recently-completed research and presents status reports of current research being performed within the Aeroelasticity Branch of the NASA Langley Research Center. Within the paper this research is classified as experimental, analytical, and theoretical aeroelastic research. The paper also describes the Langley Transonic Dynamics Tunnel, its features, capabilities, a new open-architecture data acquisition system, ongoing facility modifications, and the subsequent calibration of the facility.
Fluid-Structure Coupling Requirements for Time-Accurate Aeroelastic Simulations
Authors: Bendiksen Oddvar O. 
Published: March 2022

Abstract
In this paper we study the requirements that must be satisfied by a fluid-structure coupling scheme, in order to obtain a dynamically consistent aeroelastic code. Both spatial compatibility and time synchronization requirements must be met, to assure that the time-marching simulations exhibit the physically correct stability behavior. Inconsistent or inaccurate implementation of the fluid-structure boundary conditions can cause the aeroelastic code to converge to an incorrect aeroelastic solution. It is shown that CFD codes based on linear interpolations for the velocities cannot be fully compatible with structural FE codes that use plate or shell elements to model the wing skin. Numerical examples are presented for three different nonlinear aeroelastic models, using Euler-based aerodynamics and two different fluid-structure coupling schemes. The results indicate that for Mach numbers in the upper transonic range, past the transonic dip, the aeroelastic solution appears very sensitive to the fluid-structure coupling scheme used. In the case of the NACA 0012 model, the two different schemes studied predicted entirely different stability behaviors.
Aeroservoelasticity in Compressible Flow and Aeroelastic Scaling Considerations
Authors: Presente Eyal H. , Friedmann Peretz P. 
Published: March 2022

Abstract
Active flutter suppression of a two dimensional wing section in subsonic flow is studied. The equations of motion of a typical section are presented in nondimensional form. A two degree of freedom system, with pitch and plunge dynamics, combined with a trailing-edge control surface is considered. Aerodynamic loads are expressed in time-domain using Roger’s approximation. Linear optimal control is used to design a full-state feedback regulator for flutter suppression. Constraints on actuator deflection and rate limit the flutter envelope expansion. Aeroservoelastic scaling is addressed and parameters required for maintaining similarity between a full-scale system and its model are identified. Results illustrate system behavior in compressible flow. Approximate relations comparing an actively controlled flap with a continuously deforming airfoil, using piezoelectric actuation, are obtained and used to compare the performance of these two systems.
Multidisciplinary Optimization of Composite Wings Using Refined Structural and Aeroelastic Analysis Methodologies
Authors: Chattopadhyay Aditi , Jha Ratneshwar 
Published: March 2022

Abstract
An integrated multidisciplinary procedure has been developed for structural and aeroelastic optimization of composite wings based on refined analysis technique. A refined higher-order theory is used to analyze composite box beam, which represents the load carrying member of the wing. Unsteady aerodynamic computations are performed using a panel code based on the Doublet Lattice Method. Flutter/divergence dynamic pressure is obtained by the Laplace domain method through rational function approximation of unsteady aerodynamic loads. The objective of the optimization procedure is to minimize wing structural weight with constraints on flutter/divergence speed and stresses at the root due to the static load. Composite ply orientations and laminate thicknesses are used as design variables. The Kreisselmeier-Steinhauser function approach is used to efficiently integrate the objective function and constraints into a single envelope function. The resulting unconstrained optimization problem is solved using the Davidon-Fletcher-Powell algorithm. Numerical results are presented showing significant improvements, after optimization, compared to a reference design.
Integrated Electromechanical Devices for Active Control of Vibration and Sound
Authors: Anderson Eric H. , Holcomb Mark D. , Leo Donald J. , Bogue Adam X. , Russo Farla R. 
Published: March 2022

Abstract
Advances in transducers and electronics have made possible integrated electromechanical devices for active vibration and noise control. This paper describes one such system which makes use of piezoelectric materials. An integrated device employing piezoceramic actuators and sensors, analog electronic signal conditioning, programmable control components, and a voltage amplifier is described. Issues driving design of each functional subsystem are addressed. The device is packaged using flex circuit technology and other electronics industry methods. The means of integrating transducers and other components are noted. Test results indicating the vibration suppression capability are presented, and the considerably greater possibilities for more sophisticated control designs using the same system are summarized. Potential applications in active vibration and sound control are described, and uses of the broader technology, beyond the specific device design, are summarized.