bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic and physiological analysis identifies a gene network module highly associated with brassinosteroid regulation in hybrid sweetgum tissues differing in the capability of somatic embryogenesis
Authors: Zhao Ruirui , Qi Shuaizheng , Cui Ying , Gao Ying , Jiang Shuaifei , Zhao Jian , Zhang Jinfeng , Kong Lisheng 
Published: January 2022

Abstract
Somatic embryogenesis is a preferred method for large-scale production of forest trees due to its high propagation efficiency. In this study, hybrid sweetgum leaves with phase changes from mature to embryogenic state were selected as experimental material to study somatic embryo initiation. Embryogenicity ranged from high to low, i.e. from 45%, 25%, and 12.5% to 0, with the samples of embryogenic callus (EC), whiten leaf edge (WLI), whiten leaf (WLII), and green leaf (GL) respectively. High correlations existed between embryogenicity and endogenous brassinosteroids (BRs) (r = 0.95, p &lt; 0.05). Similarly, concentrations of endogenous BRs of the sample set correlated positively (r = 0.93, 0.99, 0.87, 0.99, 0.96 respectively, P &lt; 0.05) to expression of somatic embryo (SE)-related genes, i.e. BBM, LEC2, ABI3, PLT2, and WOX2. Hierarchical cluster and weighted gene coexpression network analysis identified modules of coexpressed genes and network in 4820 differentially expressed genes (DEGs) from All-BR-Regulated Genes (ABRG). Moreover, exogenously-supplemented epiBR, together with 2,4-D and 6-BA, increased embryogenicity of GL-sourced callus, and expression of SE- and auxin-related genes, while brassinazole (BRZ), a BR biosynthesis inhibitor, reduced embryogenicity. Evidences obtained in this study revealed that BRs involved in phase change of leaf explants and may function in regulating gene expression and enhancing auxin effects. This study successfully established protocols for inducing somatic embryogenesis from leaf explants in hybrid sweetgum, which could facilitate the propagation process greatly, and provide theoretical basis for manipulating SE competence of explants in ornamental woody plants.
Between mobile corridors and immobilizing borders: race, fixity and friction in Palestine/Israel
Authors: Manchanda Nivi , Plonski Sharri 
Published: January 2022

Abstract
This article wrestles with the question of ‘national’ borders in racial capitalism. We do so through an examination of border and capitalist corridors. We focus particularly on the Israeli border, branded and then sold to the rest of the world by the epistemic community of border-makers and interlocutors. In tracking the Israeli border and showing the implication of the experts and their markets, we ask how the border reflects and is refracted through a global order organized by the twin dictates of racism and capitalism. We are especially interested in how racialized processes of bordering, ostensibly governed by national exigencies, are transplanted on to other contexts. Two points emerge from this: in the first instance, we ask who and what enables this movement of the border. And in the second, we interrogate which logics and practices are transplanted with the border, as it is reproduced and seemingly fixed in a new place. We examine the violent ontologies that give shape and reputation to Israel's high-tech border industry, which has become a model for the ever-growing global homeland security industry. We ask: has Israel's border become an exportable commodity and who are the actors who have enabled this ‘achievement’? Related to this, what sort of occlusions and structural violence does the fetishization of the Israeli border rely on?
Researching climate justice: a decolonial approach to global climate governance
Authors: Wilkens Jan , Datchoua-Tirvaudey Alvine R C 
Published: January 2022

Abstract
This article addresses the broader question of the special issue by reflecting on the coloniality of knowledge production in a context of global climate governance. Drawing on the rationale of the special issue, we highlight key dynamics in which knowledge shape climate policies and propose a decolonial approach at the nexus of academic knowledge production and policy formation by accounting for diverse ways of knowing climate justice. To this end, the article asks how to develop a decolonial approach to researching climate justice in order to identify the meaning-in-use of climate justice by affected people in what we describe as sensitive regions of the Arctic and the Mediterranean. To this end, the article develops a research design that accounts for diverse ways of knowing. The article proceeds as follows: first, we will discuss how diverse ways of knowing are related to global climate governance and climate justice; second, we outline our practice-based research framework that addresses research ethics, decolonial approaches and norm contestation; and third, we discuss how our approach can inform not only the co-production of research in climate governance, but also current debates on climate justice.
Women and the Afghan peace and reintegration process
Authors: Rivas Althea-Maria , Safi Mariam 
Published: January 2022

Abstract
In 2010, as the Afghan government announced its intention to begin a formal peace process, there were numerous calls for women to have a seat at the table. Both mainstream and critical discourse on women and the peace process in Afghanistan, however, relied on the production of essentialized subjectivities which failed to recognize the political and social complexity of women's lives, diminished their intellectual contributions and silenced their voices. This article challenges the simultaneous hyper-visualization and silencing of Afghan women by both Afghan and international actors. Drawing on feminist and decolonial theory, we examine the ways in which the colonial mechanisms of intervention, patriarchy and the global hierarchies of knowledge production worked in tandem to marginalize Afghan women while upholding them as agents of peace. The article brings together findings from three participatory research projects on women and peace, which took place from 2010 to 2014 across eight provinces in Afghanistan. A prismed view of the participants' intersectional realities is constructed, which highlights diverse positionalities and articulations of peace, the social and political cleavages, and differentiated obstacles to their involvement in the peace and reintegration process. The concluding remarks emphasize the relevance of these findings for Afghanistan at the time of publication.
Inflammatory Bowel Disease Risk Variants Are Associated with an Increased Risk of Skin Cancer
Authors: Cushing Kelly C , Du Xiaomeng , Chen Yanhua , Stetson L C , Kuppa Annapurna , Chen Vincent L , Kahlenberg J Michelle , Gudjonsson Johann E , Vanderwerff Brett , Higgins Peter D R , Speliotes Elizabeth K 
Published: January 2022

Abstract

Background
Inflammatory bowel disease is associated with an increased risk of skin cancer. The aims of this study were to determine whether IBD susceptibility variants are also associated with skin cancer susceptibility and if such risk is augmented by use of immune-suppressive therapy.

Methods
The discovery cohort included participants in the UK Biobank. The validation cohort included participants in the Michigan Genomics Initiative. The primary outcome of interest was skin cancer, subgrouped into nonmelanoma skin cancers (NMSC) and melanoma skin cancers (MSC). Multivariable logistic regression with matched controls (3 controls:1 case) was performed to identify genomic predictors of skin malignancy in the discovery cohort. Variants with P &lt; .05 were tested for replication in the validation cohort. Validated Single nucleotide polymorphisms were then evaluated for effect modification by immune-suppressive medications.

Results
The discovery cohort included 10,247 cases of NMSC and 1883 cases of MSC. The validation cohort included 7334 cases of NMSC and 3304 cases of MSC. Twenty-nine variants were associated with risk of NMSC in the discovery cohort, of which 5 replicated in the validation cohort (increased risk, rs7773324-A [DUSP22; IRF4], rs2476601-G [PTPN22], rs1847472-C [BACH2], rs72810983-A [CPEB4]; decreased risk, rs6088765-G [PROCR; MMP24]). Twelve variants were associated with risk of MSC in the discovery cohort, of which 4 were replicated in the validation cohort (increased risk, rs61839660-T [IL2RA]; decreased risk, rs17391694-C [GIPC2; MGC27382], rs6088765-G [PROCR; MMP24], and rs1728785-C [ZFP90]). No effect modification was observed.

Conclusions
The results of this study highlight shared genetic susceptibility across IBD and skin cancer, with increased risk of NMSC in those who carry risk variants in IRF4, PTPN22, CPEB4, and BACH2 and increased risk of MSC in those who carry a risk variant in IL2RA.
Caveolin-1 Alleviates Crohn’s Disease–induced Intestinal Fibrosis by Inhibiting Fibroblasts Autophagy Through Modulating Sequestosome 1
Authors: Yu Mengli , Zhu Wei , Wang Jinhai , Chen Xueyang , He Xinjue , Lin Bingru , Cen Li , Zhou Tianyu , Lu Chao , Yu Chaohui , Sun Jing 
Published: January 2022

Abstract

Background
Intestinal fibrosis is a common complication of Crohn’s disease (CD) and is characterized by the excessive accumulation of extracellular matrix produced by activated myofibroblasts. Caveolin-1 (CAV1) inhibits fibrosis. However, limited data show that CAV1 affects intestinal fibrosis.

Methods
Human CD tissue samples were gained from patients with CD who underwent surgical resection of the intestine and were defined as stenotic or nonstenotic areas. A dextran sodium sulfate–induced mouse model of intestinal fibrosis was established. For in vitro experiments, we purchased CCD-18Co intestinal fibrosis cells and isolated and cultured human primary colonic fibroblasts. These fibroblasts were activated by transforming growth factor β administration for 48 hours. In the functional experiments, a specific small interfering RNA or overexpression plasmid was transfected into fibroblasts. The messenger RNA levels of fibrosis markers, such as α-smooth muscle actin, fibronectin, connective tissue growth factor, and collagen I1α, were determined using quantitative polymerase chain reaction. Western blot analysis was applied to detect the expression of CAV1, SQSTM1/p62 (sequestosome 1), and other fibrosis markers.

Results
In human CD samples and the dextran sodium sulfate–induced mouse model of intestinal fibrosis, we observed a downregulation of CAV1 in fibrosis-activated areas. Mechanistically, CAV1 knockdown in both human primary colonic fibroblasts and CCD-18Co cells promoted fibroblast activation, while CAV1 overexpression inhibited fibroblast activation in vitro. We found that SQSTM1/p62 positively correlated with CAV1 expression levels in patients with CD and that it was indirectly modulated by CAV1 expression. Rescue experiments showed that CAV1 decreased primary human intestinal fibroblast activation by inhibiting fibroblast autophagy through the modulation of SQSTM1/p62.

Conclusions
Our data demonstrate that CAV1 deficiency induces fibroblast activation by indirectly regulating SQSTM1/p62 to promote fibroblast autophagy. CAV1 or SQSTM1/p62 may be potential therapeutic targets for intestinal fibrosis.
Preoperative monocyte count is a predictor of recurrence after Stage I lung adenocarcinoma resection
Authors: Yoshida Chihiro , Kadota Kyuichi , Ishikawa Ryo , Go Tetsuhiko , Haba Reiji , Yokomise Hiroyasu 
Published: January 2022

Abstract

OBJECTIVES
High-grade tumours are observed even in Stage I lung adenocarcinomas. Tumour spread through air spaces (STAS) is a risk factor for recurrence after resection. However, there is no ideal predictive biomarker for STAS in high-grade Stage I lung adenocarcinoma. This study assessed the prognostic impact of the preoperative peripheral monocyte count in lung adenocarcinoma.

METHODS
We retrospectively analysed the data of 444 patients with resected Stage I lung adenocarcinoma during 2006–2016. Univariable and multivariable Cox proportional analyses of recurrence-free probability (RFP) and overall survival (OS) were used to analyze preoperative complete peripheral blood cell count data. Since monocyte count was associated with poor prognosis, the relationship between preoperative peripheral monocyte count and clinicopathological factors, including STAS, was assessed. In addition, immunohistochemical CD68 staining was performed to evaluate tumour-associated macrophages (TAMs).

RESULTS
A higher preoperative peripheral monocyte count was a predictor of lower RFP (P = 0.004) and lower OS (P &lt; 0.001). In multivariable analysis, a higher peripheral monocyte count was an independent prognostic factor for RFP and OS (hazard ratio: 1.88, 95% confidence interval: 1.07–3.31, P = 0.029; hazard ratio: 2.13, 95% confidence interval: 1.22–3.75, P = 0.008, respectively). A higher peripheral monocyte count was associated with a higher frequency of STAS (P = 0.017) and higher number of CD68+ TAMs (P = 0.013).

CONCLUSIONS
A higher preoperative peripheral monocyte count was an independent marker for a poor prognosis in Stage I lung adenocarcinoma and was associated with a higher frequency of STAS.
Understanding the Potential Drivers for Respiratory Syncytial Virus Rebound During the Coronavirus Disease 2019 Pandemic
Authors: Li You , Wang Xin , Cong Bingbing , Deng Shuyu , Feikin Daniel R , Nair Harish 
Published: January 2022

Abstract
Nonpharmaceutical interventions (NPIs) were widely introduced to combat the coronavirus disease 2019 (COVID-19) pandemic. These interventions also likely led to substantially reduced activity of respiratory syncytial virus (RSV). From late 2020, some countries observed out-of-season RSV epidemics. Here, we analyzed the role of NPIs, population mobility, climate, and severe acute respiratory syndrome coronavirus 2 circulation in RSV rebound through a time-to-event analysis across 18 countries. Full (re)opening of schools was associated with an increased risk for RSV rebound (hazard ratio [HR], 23.29 [95% confidence interval {CI}, 1.09–495.84]); every 5°C increase in temperature was associated with a decreased risk (HR, 0.63 [95% CI, .40–.99]). There was an increasing trend in the risk for RSV rebound over time, highlighting the role of increased population susceptibility. No other factors were found to be statistically significant. Further analysis suggests that increasing population susceptibility and full (re)opening of schools could both override the countereffect of high temperatures, which explains the out-of-season RSV epidemics during the COVID-19 pandemic.
New Dimensions of Global Feminist Influence: Tracking Feminist Mobilization Worldwide, 1975–2015
Authors: Forester Summer , Kelly-Thompson Kaitlin , Lusvardi Amber , Weldon S Laurel 
Published: January 2022

AbstractFeminist mobilization, crucial for advancing women's human rights, has increased in all world regions since 1975. However, we do not know enough about the global impact of this mobilization because we lack adequate databases to explore the ways that feminist mobilization interacts with other factors that enhance and limit women's rights, such as democracy, intergovernmental processes, and transnational, regional organizing. Our ability to explore these questions is obstructed by a lack of data on the global south and measures that focus on formal organizations. This project remedies these gaps, developing an improved measure of feminist mobilization that encompasses autonomous, domestic feminist mobilization in 126 countries, 1975–2015, enabling us to track global and regional trends. Using regional comparisons and statistical analysis, we use this new measure to reveal new patterns and complexities in feminist mobilization. We discern distinct regional patterns in such organizing that defy facile predictions of global convergence and suggest a central role for UN processes advancing women's rights. Our analysis also points to the importance of transnational feminist networks and democratization as factors enabling and strengthening feminist mobilization. We conclude by suggesting some fruitful avenues for exploring relationships between feminist movements, international institutions, and democracy.
The impact of the COVID-19 pandemic on antimicrobial prescribing at a specialist paediatric hospital: an observational study
Authors: Vestesson Emma , Booth John , Hatcher James , McGarrity Orlagh , Sebire Neil J. , Steventon Adam , Suarez Alonso Carlos , Tomlin Stephen , Standing Joseph F. 
Published: January 2022

Abstract

Background
The COVID-19 pandemic has severely impacted healthcare delivery and there are growing concerns that the pandemic will accelerate antimicrobial resistance.

Objectives
To evaluate the impact of the COVID-19 pandemic on antibiotic prescribing in a tertiary paediatric hospital in London, UK.

Methods
Data on patient characteristics and antimicrobial administration for inpatients treated between 29 April 2019 and Sunday 28 March 2021 were extracted from the electronic health record (EHR). Interrupted time series analysis was used to evaluate antibiotic days of therapy (DOT) and the proportion of prescribed antibiotics from the WHO ‘Access’ class.

Results
A total of 23 292 inpatient admissions were included. Prior to the pandemic there were an average 262 admissions per week compared with 212 during the pandemic period. Patient demographics were similar in the two periods but there was a shift in the specialities that patients had been admitted to. During the pandemic, there was a crude increase in antibiotic DOTs, from 801 weekly DOT before the pandemic to 846. The proportion of Access antibiotics decreased from 44% to 42%. However, after controlling for changes in patient characteristics, there was no evidence for the pandemic having an impact on antibiotic prescribing.

Conclusions
The patient population in a specialist children’s hospital was affected by the COVID-19 pandemic, but after adjusting for these changes there was no evidence that antibiotic prescribing was significantly affected by the pandemic. This highlights both the value of routine, high-quality EHR data and importance of appropriate statistical methods that can adjust for underlying changes to populations when evaluating impacts of the pandemic on healthcare.
The Challenge of Measuring Sweet Taste in Food Ingredients and Products for Regulatory Compliance: A Scientific Opinion
Authors: Starkey Dustin E , Wang Zhuzhu , Brunt Kommer , Dreyfuss Lise , Haselberger Philip A , Holroyd Stephen E , Janakiraman Kaushik , Kasturi Prabhakar , Konings Erik J M , Labbe David , Latulippe Marie E , Lavigne Xavier , McCleary Barry V , Parisi Salvatore , Shao Tony , Sullivan Darryl , Torres Marina , Yadlapalli Sudhakar , Vrasidas Ioannis 
Published: January 2022

Abstract
The Codex Alimentarius Commission, a central part of the joint Food and Agricultural Organization/World Health Organizations Food Standards Program, adopts internationally recognized standards, guidelines, and code of practices that help ensure safety, quality, and fairness of food trade globally. Although Codex standards are not regulations per se, regulatory authorities around the world may benchmark against these standards or introduce them into regulations within their countries. Recently, the Codex Committee on Nutrition and Foods for Special Dietary Uses (CCNFSDU) initiated a draft revision to the Codex standard for follow-up formula (FUF), a drink/product (with added nutrients) for young children, to include requirements for limiting or measuring the amount of sweet taste contributed by carbohydrates in a product. Stakeholders from multiple food and beverage manufacturers expressed concern about the subjectivity of sweetness and challenges with objective measurement for verifying regulatory compliance. It is a requirement that Codex standards include a reference to a suitable method of analysis for verifying compliance with the standard. In response, AOAC INTERNATIONAL formed the Ad Hoc Expert Panel on Sweetness in November 2020 to review human perception of sweet taste, assess the landscape of internationally recognized analytical and sensory methods for measuring sweet taste in food ingredients and products, deliver recommendations to Codex regarding verification of sweet taste requirements for FUF, and develop a scientific opinion on measuring sweet taste in food and beverage products beyond FUF. Findings showed an abundance of official analytical methods for determining quantities of carbohydrates and other sweet-tasting molecules in food products and beverages, but no analytical methods capable of determining sweet taste. Furthermore, sweet taste can be determined by standard sensory analysis methods. However, it is impossible to define a sensory intensity reference value for sweetness, making them unfit to verify regulatory compliance for the purpose of international food trade. Based on these findings and recommendations, the Codex Committee on Methods of Analysis and Sampling agreed during its 41st session in May 2021 to inform CCNFSDU that there are no known validated methods to measure sweetness of carbohydrate sources; therefore, no way to determine compliance for such a requirement for FUF.
Qualitative Screening of Amphetamine- and Ketamine-Type Abuse Drugs in Urine Employing Dual Mode Extraction Column by Liquid Chromatography–Tandem Mass Spectrometry (LC–MS-MS)
Authors: Wong George Fai , Lee Wing-Man , Li Chi-Keung 
Published: January 2022

Abstract
This manuscript reported a fast and rapid qualitative screening method for abuse drugs in urine by liquid chromatography–tandem mass spectrometry (LC–MS-MS). The scope of the abuse drugs under investigation included methamphetamine (MA), amphetamine (AMP), methylenedioxymethamphetamine (MDMA), methylenedioxyamphetamine (MDA), paramethoxymethamphetamine (PMMA), ephedrine, pseudoephedrine, ketamine (KET), deschloroketamine (DCK), 2-fluorodeschloroketamine (2 F-DCK) and deschloro-N-ethylketamine (2-oxo-PCE). The method employed a dual mode extraction (DME) column as a novel clean-up method for the urine matrix. To an aliquot of 0.2 mL urine, internal standards (ISTDs) and 0.4 mL of acidified methanol were added. After vortex and centrifugation, the supernatant was passed through a DME column before LC–MS-MS analysis. Chromatographic separation was achieved with a C18 column by gradient elution. The limits of detection (LODs) for MA, AMP, MDMA, MDA and PMMA were 3 ng/mL, whereas those for ephedrine and pseudoephedrine were 10 ng/mL and those for KET, DCK, 2 F-DCK and 2-oxo-PCE were 1 ng/mL. The matrix effects ranged from −12% to 7% (%CV from 4% to 19%). This method is fit for the intended purpose for forensic toxicology, as well as for forensic analysis of drugs facilitating sexual assault and other criminal acts.