bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Formation Mechanism of Epigallocatechin - 5-Hydroxymethylfurfural Complexes by Molecular Simulation Combined with Spectroscopy Techniques
Authors: Man Ziyi , Chen Ruihuan , Feng Yi , Xiao Jibo , Yang Hailong , Wu Xiangting 
Published: July 2022

Abstract
The complexes of epigallocatechin (EGC) and 5-hydroxymethylfurfural (5-HMF) was constructed in water-soluble and ethanol-soluble systems to study the effect of EGC on the chemical structure of food harmful substance 5-HMF. The results showed that EGC and 5-HMF can be combined by non-covalent way in water-soluble and ethanol-soluble systems, and the main force was hydrogen bond. Scanning electron microscope, X-ray diffractometer and differential scanning calorimeter displayed that EGC combined with 5-HMF in the form of co-amorphous complexes. Fourier transform infrared spectroscopy, 1H-NMR spectroscopy and molecular dynamics simulation confirmed that EGC can form O-H...O hydrogen bonds with adjacent 5-HMF molecules. Thin-layer chromatography and in vitro digestive juice assay further proved that no free 5-HMF was observed after EGC and 5-HMF formed complexes. The results indicated that EGC and 5-HMF formed an EGC-5-HMF co-amorphous complexes through intermolecular hydrogen bonding, which could change the stability of free 5-HMF and EGC presents favorable potential for the control of 5-HMF in food.
Mixed artificial reasoning, closer to human?
Authors: Ghidalia Sarah , Narsis Ouassila Labbani , Bertaux Aurélie , Nicolle Christophe 
Published: July 2022

Abstract
AI is now used in many areas of our daily life, however we still have difficulties to make machines interact with our world as we perceive it. Humans mainly use two types of reasoning to grasp reality: inductive and deductive reasoning. In AI, inductive reasoning is possible thanks to the use of machine learning models, while deductive reasoning can be provided by the use of ontologies. Based on these statements, we have conducted a review of articles combining the use of machine learning and ontologies in order to identify the most recent techniques that combine induction and deduction within an AI. This systematic literature review allowed us, after reading 128 studies, to identify three main groups of hybridization between machine learning and ontologies: learning-enhanced ontologies, semantic data mining and learning and reasoning system. The combination of these two types of reasoning allows machines to be better informed about the specificity of our world.
New Targets of TetR-type regulator SLCG_2919 for Controlling Lincomycin Biosynthesis in Streptomyces lincolnensis
Authors: Xu Yurong , Yi Jing , Li Binglin , Li Xiaowei , Gao Yang , Cheng Shuhan , Zhou Qihua , Wu Hang , Zhang Buchang 
Published: July 2022

Abstract
The transcription factor (TF)-mediated regulatory network controlling lincomycin production in Streptomyces lincolnensis is yet to be fully elucidated despite several types of associated TFs having been reported. SLCG_2919, a tetracycline repressor (TetR)-type regulator, was the first TF to be characterized outside the lincomycin biosynthetic cluster to directly suppress the lincomycin biosynthesis in S. lincolnensis. In this study, improved genomic systematic evolution of ligands by exponential enrichment (gSELEX), an in-vitro technique, was adopted to capture additional SLCG_2919-targeted sequences harbouring the promoter regions of SLCG_6675, SLCG_4123-4124, SLCG_6579, and SLCG_0139-0140. These results were confirmed by electrophoretic mobility shift assays (EMSAs). RT-qPCR showed that the corresponding target genes SLCG_6675 (anthranilate synthase), SLCG_0139 (LysR family transcriptional regulator), SLCG_0140 (beta-lactamase), SLCG_6579 (cytochrome P450), SLCG_4123 (bifunctional DNA primase/polymerase), and SLCG_4124 (magnesium or magnesium-dependent protein phosphatase) in ΔSLCGL_2919 were differentially increased by 3.3-, 4.2-, 3.2-, 2.5-, 4.6-, and 2.2-fold relative to those in the parental strain S. lincolnensis LCGL. Furthermore, the individual inactivation of these target genes in LCGL reduced the lincomycin yield to varying degrees. This investigation expands on the known DNA targets of SLCG_2919 to control lincomycin production, and lays the foundation for improving industrial lincomycin yields via genetic engineering of this regulatory network.
Association Between Occupational Noise Exposure and Benign Vocal Fold Lesions in South Korea: A Nationwide Population‐Based Study
Authors: Kim Ji-Sun , Kim Sukil , Moon Tae-Hoon , Park Soomin , Kim Seung Hwa , Kim Subin , Lee Dong-Hyun , Kim Byung Guk , Chang Ki-Hong , Park Jun-Ook 
Published: July 2022

Abstract
Background: Voice abuse in noisy environments can result in voice disorders. However, there are insufficient studies to differentiate vocal cord lesions through laryngoscopic examination for workers in noisy environments. This study investigated the relationship between a history of noise exposure in the workplace and benign vocal fold lesions (BVFLs).Methods: We used Korean National Health and Nutrition Examination Survey data from 2010 to 2012. Chi-square testing was used to compare characteristics between two groups according to the presence or absence of BVFLs. To investigate the association between BVFLs and noise exposure in the workplace, we calculated adjusted odds ratios and 95% confidence intervals (CIs) using multiple logistic regression analyses.Results: 10,170 participants with available laryngoscopy results were enrolled. Smoking history, hypertension, diabetes, and exposure to noise for more than 3 months at the workplace were significantly higher in the group with BVFLs. After adjusting for age, sex, smoking, drinking, obesity, hypertension, diabetes, income, education, and occupation as confounders, we confirmed that BVFLs were 1.52 times more likely to occur in the group with occupational noise exposure (95% CI: 1.157–1.990).Conclusions: Working in a noisy environment could induce BVFLs in workers through voice abuse. Social recognition that a noisy environment is a risk factor for BVFLs needs to be improved, and prevention measures should be implemented.
Low expression of Isocitrate Dehydrogenase 1 (IDH1) R132H is associated with advanced pathological features in laryngeal squamous cell carcinoma
Authors: Shayanfar Nasrin , Zare-Mirzaie Ali , Mohammadpour Mahsa , Jafari Ensieh , Mehrtash Amirhosein , Emtiazi Nikoo , Tajik Fatemeh 
Published: July 2022

Abstract
Introduction:
Recent developments in genomic sequencing have led to the identification of somatic mutations in isocitrate dehydrogenase 1 (IDH1) in various malignancies. IDH1 R132H is the most common mutation of IDH1, which affects codon 132 and results in the conversion of amino acid residue arginine (R) to histidine (H). The expression and clinical significance of IDH1 R132H in laryngeal squamous cell carcinoma (LSCC) have not been explored.
Methods
The expression pattern and clinical significance of IDH1 R132H were investigated in tissue microarrays (TMAs) of 50 LSCC tumors as well as adjacent normal tissues using immunohistochemistry (IHC). Then the exon of the 12 tumor samples with negative/weak positive staining were sequenced by applying polymerase chain reaction (PCR).
Results
The results demonstrated that the cytoplasmic expression of IDH1 R132H was downregulated in tumor cells compared to adjacent normal tissues. A statistically significant association was found between a low level of cytoplasmic expression of IDH1 R132H protein and advanced histological grade (p = 0.000), perineural invasion (p = 0.019), and lymph node involvement (p = 0.000). The exon4 sequencing results showed that only one sample was positive for IDH1 R132H mutation. IDH1 R132H expression was observed in 39 (78.0%) LSCC samples.
Conclusion
These findings indicate that low cytoplasmic expression of IDH1 R132H has a clinical significance and is associated with more aggressive tumor behavior and progression of LSCC, which can help improve potential treatment in LSCC patients. Further investigations are required to understand the biological function of marker in LSCC.
The effect of filters and varying illumination on contrast sensitivity in eyes with moderate to severe visual impairment
Authors: Abraham Carl Halladay , Morny Enyam , Aboagye-MacCarthy Asantewaa , Ocansey Stephen , Ntodie Michael , Sakyi-Badu Godfred , Dadzie Albert Kofi , Addo Naa Adjeley , Holdbrook Selina , Abu Emmanuel Kwasi 
Published: July 2022

Abstract
Purpose: To investigate the effect of filters and illumination on contrast sensitivity in persons with cataract, pseudophakia, maculopathy and glaucoma to provide a guide for eye care providers in low vision rehabilitation.Materials and methods: A within-subjects experimental design with a counter-balanced presentation technique was employed in this study. The contrast sensitivity of eyes with cataract, pseudophakia, maculopathy and glaucoma were measured with filters (no filter, yellow, pink and orange) combined with increasing illumination levels (100lux, 300lux, 700lux and 1000lux) using the SpotChecksTM contrast sensitivity chart. The data was analyzed using descriptive statistics and two-way repeated measures ANOVA.Results: The yellow filter in combination with 100lux significantly (p<0.01) improved contrast sensitivity by approximately 6.5% among eyes with cataract. There was also a significant interaction between illumination and filters, with Huynh-Feldt correction, F (7.20, 230.23) = 2.06, p=0.04. Eyes with pseudophakia performed best at 1000lux with or without filters. In eyes with maculopathy, the yellow filter combined with 300lux gave the best improvement in CS from 1.33-1.45logCS (p=0.01). Illumination of 1000lux without a filter improved CS best in eyes with Glaucoma.Conclusion: The effect of filters and illumination on contrast sensitivity is dependent on the cause of contrast sensitivity loss. Eye care professionals must consider the underlying cause of visual impairment if patient specific care is to be rendered.
Autonomic stress response of physiotherapy student in the different scenarios of an objective structured clinical examination
Authors: Martínez-Pascual Beatriz , Ramírez-Adrados Ana , Fernández-Martínez Silvia , Gonzalez-de-Ramos Cristina , Fernández-Elías Valentín E. , Clemente-Suárez Vicente J 
Published: July 2022

Abstract
The aim of the present research was to analyse modifications in the autonomic stress response of Physiotherapy students undergoing a 12-scenario Objective Structured Clinical Evaluation (OSCE). A total of 86 last year students of the Physiotherapy bachelor’s degree ( 27.29 years (SD = 6.66).; 36 females and 50 males) randomly assigned were monitored during the complete OSCE to measure heart rate variability (HRV) in temporal, frequency, and non-linear domains. The HRV analysed showed a large anticipatory stress response of students maintained during the entire evaluation. The stress response varied regarding OSCE station complexity and demands and the highest sympathetic response was not found in higher emotional scenarios. The autonomic modulation monitoring allows teachers to design OSCE scenarios more adapted to the students, limiting the effect of the stress response to allow a better performance.
Multi-party Non-interactive Atomic Fair Data Exchange based on Blockchain
Authors: Zhan Jing , Li Yongzhen , Zhao Jiang , Gao Wei , Li Bo , Zhao Yong 
Published: July 2022

Abstract
Widely adopted blockchain-based fair data exchange protocol have the following problems in multi-party scenario: 1) in multi-buyer v.s. one seller scenario, the negotiation for data availability verification between the buyer and seller reduces transaction throughput greatly. Besides, malicious buyers can launch DoS attacks to prevent the seller from serving others by requiring lots of data availability proofs. 2) in multi-seller v.s. one buyer scenario where the buyer pays for the merged data of all sellers, current protocols treat this as multiple independent transactions, resulting in excessive on-chain costs. Moreover, current protocols neglect that data ownership establishment on-chain may be tampered since the registration info is in plaintext and submitted through the Internet.

This paper proposes multi-party non-interactive atomic fair data exchange protocol based on blockchain to solve the above problems, providing data confidentiality, transaction atomic fairness, data intellectual property right protection, and high efficiency. Specifically, we propose transparent zero knowledge proof-based data verification guaranteeing the data confidentiality and transaction fairness. With the proof computed once and used everywhere, transaction throughput is improved greatly and DoS attacks initiated by malicious buyers is prevented. Moreover, the agent representing multi-seller is introduced to reduce on-chain costs. Furthermore, two-stage on-chain ownership registration is proposed to prevent eavesdroppers from impersonating the owner. Finally, we implement a POC (Proof of Concept) of our protocol as the BADE (Blockchain-based multi-party non-interactive Atomic fair Data Exchange). Experiments show that, our throughput within 12 hours is 50 times that of existing solution\cite{ref10}. And in multi-seller scenario, the on-chain gas costs of our protocol is reduced by 19.9\%-30.5\% in different seller/buyer ratios. The fairness of data exchange is also ensured by extra gas costs of dishonest party.
Can they stomach it? Parent and practitioner acceptability of a trial comparing Gastric Residual Volume measurement versus no Gastric Residual Volume in UK NNU and PICU’s
Authors: Deja Elizabeth , Roper Louise , Tume Lyvonne , Dorling Jon , Gale Chris Gale , Arch Barbara , Latten Lynne ,   Nazima Pathan7 Pathan, Eccleson Helen Eccleson , Hickey Helen , Preston Jenny , Beissel Anne , Andrzejewska Izabela , Valla Frédéric Valla V , Woolfall Kerry 
Published: July 2022

Abstract

Background Routine measurement of gastric residual volume (GRV) to guide feeding in neonatal and paediatric intensive care is widespread. However, this practice is not evidence based and may cause harm. We explored parent and practitioner views on the acceptability of a trial comparing GRV measurement or no GRV measurement.Methods A mixed- methods study involving interviews and focus groups with practitioners and interviews with parents with experience of tube feeding in neonatal and/or paediatric intensive care. A voting system recorded closed question responses during practitioner data collection, enabling the collection of quantitative and qualitative data. Data were analysed using thematic analysis and descriptive statistics.Results We interviewed 31 parents and nine practitioners and ran five practitioner focus groups (n = 42). Participants described how the research question was logical, the intervention would not be invasive and potential benefits of not withholding the child’s feeds. However, both groups held concerns about the potential risk of not measuring GRV, including delayed diagnosis of infection and gut problems, increased risk of vomiting into lungs and causing discomfort or pain. Parent’s views on GRV measurement and consent decision making were influenced by their views on the importance of feeding in the ICU, their child’s prognosis and associated comorbidities or complications.Conclusions The majority of parents and practitioners viewed the proposed trial as acceptable. Potential concerns and preferences were identified that will need careful consideration to inform the development of the proposed trial protocol and staff training.
Belgian rare diseases plan in clinical pathology: identification of key biochemical diagnostic tests and establishment of reference laboratories and financing conditions
Authors: Vandevelde Nathalie Monique , Vermeersch Pieter , Devreese Katrien M.J. , Vincent Marie-Françoise , Gulbis Béatrice , Eyskens François , Boemer François , Gothot André , Hoof Viviane O. Van , Bonroy Carolien , Stepman Hedwig , Martens Geert A. , Bossuyt Xavier , Roosens Laurence , Smet Julie , Laeremans Hilde , Weets Ilse , Minon Jean-Marc , Vernelen Kris , Coucke Wim ,   Board of the Action 1 of the Belgian National Plan for Rare Diseases
Published: July 2022

Abstract

Background: One objective of the Belgian Rare Diseases plan is to improve patients’ management using phenotypic tests and, more specifically, the access to those tests by identifying the biochemical analyses used for rare diseases, developing new financing conditions and establishing reference laboratories.Methods: A feasibility study was performed from May 2015 until August 2016 in order to select the financeable biochemical analyses, and, among them, those that should be performed by reference laboratories. This selection was based on an inventory of analyses used for rare diseases and a survey addressed to the Belgian laboratories of clinical pathology (investigating the annual analytical costs, volumes, turnaround times and the tests unavailable in Belgium and outsourced abroad). A proposal of financeable analyses, financing modalities, reference laboratories’ scope and budget estimation was developed and submitted to the Belgian healthcare authorities. After its approval in December 2016, the implementation phase took place from January 2017 until December 2019.Results: In 2019, new reimbursement conditions have been published for 46 analyses and eighteen reference laboratories have been recognized. Collaborations have also been developed with 5 foreign laboratories in order to organize the outsourcing and financing of 9 analyses unavailable in Belgium. Conclusions: In the context of clinical pathology and rare diseases, this initiative enabled to identify unreimbursed analyses and to meet the most crucial financial needs. It also contributed to improve patients’ management by establishing Belgian reference laboratories and foreign referral laboratories for highly-specific analyses and a permanent surveillance, quality and financing framework for those tests.
Bayesian method for estimating Weibull parameters for wind resource assessment in the Equatorial region: a comparison between two-parameter and three-parameter Weibull distributions
Authors: Khan M. G. M. , Ahmed M. Rafiuddin 
Published: July 2022

Abstract
The two-parameter Weibull distribution has garnered much attention in the assessment of windenergy potential. The estimation of the shape and scale parameters of the distribution has broughtforth a successful tool for the wind energy industry. However, it may be inappropriate to use thetwo-parameter Weibull distribution to assess energy at every location, especially at sites wherelow wind speeds are frequent, such as the Equatorial region. In this work, a robust technique inwind resource assessment using a Bayesian approach for estimating Weibull parameters is firstproposed. Secondly, the wind resource assessment techniques using a two-parameter Weibulldistribution and a three-parameter Weibull distribution which is a generalized form of twoparameterWeibull distribution are compared. Simulation studies confirm that the Bayesianapproach seems a more robust technique for accurate estimation of Weibull parameters. Theresearch is conducted using data from seven sites in Equatorial region from 1o N of Equator to 19oSouth of Equator. Results reveal that a three-parameter Weibull distribution with non-zero shiftparameter is a better fit for wind data having a higher percentage of low wind speeds (0-1 m/s) andlow skewness. However, wind data with a smaller percentage of low wind speeds and highskewness showed better results with a two-parameter distribution that is a special case of threeparameterWeibull distribution with zero shift parameter. The results also demonstrate that theproposed Bayesian approach and application of a three-parameter Weibull distribution areextremely useful in accurate estimate of wind power and annual energy production.
Speech Processing as a Far-Transfer Gauge of Serious Games for Cognitive Training in Aging: Randomized Controlled Trial of Web-Based Effectivate Training
Authors: Nitsan Gal , Baharav Shai , Tal-Shir Dalith , Shakuf Vered , Ben-David Boaz M 
Published: July 2022

Background
The number of serious games for cognitive training in aging (SGCTAs) is proliferating in the market and attempting to combat one of the most feared aspects of aging—cognitive decline. However, the efficacy of many SGCTAs is still questionable. Even the measures used to validate SGCTAs are up for debate, with most studies using cognitive measures that gauge improvement in trained tasks, also known as near transfer. This study takes a different approach, testing the efficacy of the SGCTA—Effectivate—in generating tangible far-transfer improvements in a nontrained task—the Eye tracking of Word Identification in Noise Under Memory Increased Load (E-WINDMIL)—which tests speech processing in adverse conditions.

Objective
This study aimed to validate the use of a real-time measure of speech processing as a gauge of the far-transfer efficacy of an SGCTA designed to train executive functions.

Methods
In a randomized controlled trial that included 40 participants, we tested 20 (50%) older adults before and after self-administering the SGCTA Effectivate training and compared their performance with that of the control group of 20 (50%) older adults. The E-WINDMIL eye-tracking task was administered to all participants by blinded experimenters in 2 sessions separated by 2 to 8 weeks.

Results
Specifically, we tested the change between sessions in the efficiency of segregating the spoken target word from its sound-sharing alternative, as the word unfolds in time. We found that training with the SGCTA Effectivate improved both early and late speech processing in adverse conditions, with higher discrimination scores in the training group than in the control group (early processing: F1,38=7.371; P=.01; ηp2=0.162 and late processing: F1,38=9.003; P=.005; ηp2=0.192).

Conclusions
This study found the E-WINDMIL measure of speech processing to be a valid gauge for the far-transfer effects of executive function training. As the SGCTA Effectivate does not train any auditory task or language processing, our results provide preliminary support for the ability of Effectivate to create a generalized cognitive improvement. Given the crucial role of speech processing in healthy and successful aging, we encourage researchers and developers to use speech processing measures, the E-WINDMIL in particular, to gauge the efficacy of SGCTAs. We advocate for increased industry-wide adoption of far-transfer metrics to gauge SGCTAs.