bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on the Intervention Program of Dance Therapy on the Joint Attention of Children with Autism
Authors: Liu Shanshan 
Published: December 2022

In recent years, with the development of the concept of Autism Spectrum Disorder (ASD) and the exploration of related research, it has been found that the common attention of autistic children is regarded as the core defect. As a kind of psychotherapy, dance therapy can mainly promote the integration of emotion, cognition and the sociality of autistic children through the media of "body/movement". This paper is made with the main aims in improving the common attention of autistic children to study the intervention program of dancing therapy.
PENEGAKAN HUKUM SANKSI ADMINISTRASI TERHADAP PELANGGARAN PERIZINAN
Authors: Yonnawati Yonnawati 
Published: December 2022

Sanksi merupakan bagian penutup yang penting di dalam hukum.Salah satu sanksi yang dapat diterapkan terhadap suatu pelanggaran atas peraturan perundang-undangan adalah sanksiadministrasi. Sanksiini merupakan suatu bentuk pemaksaan dari administrasi negara(pemerintah) terhadapwarga negara dalamhaladanya perintah-perintah,kewajiban kewajiban, atau larangan-larangan yang diatur dalam peraturan perundang-undangan yang dikeluarkan oleh administrasi negara (pemerintah)termasuk didalamnya peraturanperundang-undangan bidang perizinan. Sanksi administrasi yang dapat dikenakan terhadap pelanggaran perizinan dapat berupa paksaan Pemerintahan (bestuurdwang), penarikan kembali keputusanyang menguntungkan, pengenaan uang paksa oleh Pemerintah (dwangsom), pengenaan denda administratif (administratif boete).Penetapan sanksi administrasi terhadap pelanggaran di bidang perizinan bentuknya bermacam-macam yang pada umumnya sudah secara definitif tercantum dalam peraturan perundang- undangan yang menjadi dasarnya.
Efeitos da plataforma vibratória na densidade mineral óssea em mulheres pós-menopausa: uma revisão sistemática
Authors: Santos Naiala de Jesus Silva , Barbosa Ramon Martins , Santos Everton Carvalho dos , Gomes Vinícius Afonso 
Published: December 2022

Introdução: Revisar estudos que analisaram os efeitos da plataforma vibratória sobre a densidade mineral óssea em mulheres na pós-menopausa. Métodos: Revisão sistemática, PROSPERO (CRD42020173020), de artigos publicados nas bases Pubmed, PEDro e Portal da BVS. Descritores: “Vibration”, “Bone Density”, “Women”, “Osteoporosis”, “Postmenopausal” e “Clinical Trial”. Incluídos: 1) Ensaios clínicos randomizados; 2) que analisaram os efeitos da plataforma vibratória na densidade mineral óssea; 3) em mulheres pós-menopausa; 4) disponíveis na íntegra. Excluídos: 1) ausência dos parâmetros frequência, tempo de exposição e posição corporal e, 2) teses e dissertações de mestrado/doutorado. A qualidade metodológica (risco de viés) foi avaliada com a escala PEDro e ferramenta de risco de viés da Cochrane. Resultados: As buscas identificaram 1.108 estudos, contudo, 7 foram incluídos. Eram ensaios clínicos randomizados, publicados entre 2006 e 2020. A amostra totalizou 509 mulheres pós-menopáusicas. Dessas, 292 utilizaram a plataforma vibratória, e 217 do grupo controle e/ou outras intervenções. O tempo desde a menopausa variou entre 1 e 12 anos. O protocolo de intervenção, variou entre 12,5 e 90 Hz, com tempo de exposição entre 5 e 60 minutos, com duração de 4 a 12 meses. Os resultados sugerem que a plataforma vibratória promoveu melhoras e/ou manutenção na densidade mineral óssea do fêmur, coluna lombar e cervical em mulheres pós-menopausa. Na análise metodológica, a maioria dos estudos possuem moderado risco de viés. Conclusão: A plataforma vibratória promove aumento/manutenção na densidade mineral óssea em mulheres pós-menopáusicas, podendo acarretar em redução das quedas e diminuição do risco de hospitalização.
Pengenalan Potensi Diri Remaja Peserta Katekumen GMIT Jemaat PNIEL Manutapen Klasis Kota Kupang sebagai Upaya Mempersiapkan Karir
Authors: Keo Glorius Deonatus , Lete Mira , Adoe Chintamy , Natonis Etrin , Lian Mona , Punga Aston , Maleng Getroida , Nakmofa Maria , Naif Ardi 
Published: December 2022

Suatu cara mengenali potensi diri remaja yang mengikuti kelas katekisasi adalah melalui kegiatan pelatihan potensi diri remaja. Manfaat yang didapatkan dari kegiatan pengabdian kepada masyarakat ini antara lain Remaja dapat mengenal bakat dan minatnya yang berguna untuk penentuan karir dan masa depannya. Selain itu, remaja juga akan bertumbuh menjadi pribadi dewasa yang dapat melakukan aktualisasi kemampuannya dalam karir, dalam keluarga dan dalam kehidupan sosialnya. Kegiatan ini dilaksanakan di GMIT Jemaat PNIEL Manutapen - Klasis Kota Kupang. Kegiatan Pelatihan ini dihadiri oleh peserta katekisasi yang adalah berjumlah 71 orang remaja. Terdapat tiga tahapan dalam kegiatan ini antara lain: 1) persiapan, 2) pelaksanaan dan 3) Evaluasi. Hasil dari pelatihan ini membuat peserta memahami dan mengenal potensi diri mereka, peserta juga memahami kecerdasan dan kepribadian mereka serta peserta mampu menentukan karir kedepan.
Application of polymerized porcine hemoglobin in the ex vivo normothermic machine perfusion of rat livers
Authors: Li Bin , Zhang Jie , Shen Chuanyan , Zong Tingting , Zhao Cong , Zhao Yumin , Lu Yunhua , Sun Siyue , Zhu Hongli 
Published: December 2022

Background: In contrast to traditional static cold preservation of donor livers, normothermic machine perfusion (NMP) may reduce preservation injury, improve graft viability and potentially allows ex vivo assessment of graft viability before transplantation. The polymerized porcine hemoglobin is a kind of hemoglobin oxygen carrier prepared by crosslinking porcine hemoglobin by glutaraldehyde to form a polymer. The pPolyHb has been proved to have the ability of transporting oxygen which could repair the organ ischemia-reperfusion injury in rats.Objective: In order to evaluate the effectiveness of rat liver perfusion in vitro based on pPolyHb, we established the NMP system, optimized the perfusate basic formula and explored the optimal proportion of pPolyHb and basal perfusate.Methods: The liver was removed and perfused for 6 h at 37°C. We compared the efficacy of liver perfusion with different ratios of pPolyHb. Subsequently, compared the perfusion effect using Krebs Henseleit solution and pPolyHb perfusate of the optimal proportion, and compared with the liver preserved with UW solution. At 0 h, 1 h, 3 h and 6 h after perfusion, appropriate samples were collected for blood gas analysis and liver injury indexes detection. Some tissue samples were collected for H&amp;E staining and TUNEL staining to observe the morphology and detect the apoptosis rate of liver cells. And we used Western Blot test to detect the expression of Bcl-2 and Bax in the tissues.Results: According to the final results, the optimal addition ratio of pPolyHb was 24%. By comparing the values of Bcl-2/Bax, the apoptosis rate of pPolyHb group was significantly reduced. Under this ratio, the results of H&amp;E staining and TUNEL staining showed that the liver morphology was well preserved without additional signs of hepatocyte ischemia, biliary tract injury, or hepatic sinusoid injury, and hepatocyte apoptosis was relatively mild.Conclusion: Through the above-mentioned study we show that within 6 h of perfusion based on pPolyHb, liver physiological and biochemical activities may essentially be maintained in vitro. This study demonstrates that a pPolyHb-based perfusate is feasible for NMP of rat livers. This opens up a prospect for further research on NMP.
The oral microbiome in young women at different stages of periodontitis: Prevotella dominant in stage III periodontitis
Authors: Zhao Yaqiong , Feng Yunzhi , Ye Qin , Hu Jing , Feng Yao , Ouyang Zeyue , Zhao Jie , Chen Yun , Tan Li , Chen Ningxin , Dusenge Marie Aimee , Su Xiaolin , Guo Yue 
Published: December 2022

ObjectivePeriodontitis progression is related to the dynamic dysbiosis of oral microbiome. We identified the dominant bacteria and the potential pathway in young women with stage-III periodontitis.Materials and methodsSamples of subgingival plaque were collected from 26 young women with periodontitis (20 with stage-I and 6 with stage-III). Using 16S rRNA-sequencing, we determined the variation in oral bacterial communities of the two groups, and identified the dominant bacteria of each group. We used the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to evaluate the signaling pathways related to the difference in oral bacterial composition. The role of the dominant bacteria of stage-III periodontitis was investigated in vivo and in vitro using an endoplasmic reticulum stress inhibitor.ResultsYoung women with stage-I periodontitis had higher values for the Chao1 Index, Observed Species and Phylogenetic Diversity Whole Tree Index than those for women with stage-III periodontitis. β-diversity analyses revealed that samples could be divided into different groups according to the periodontitis stage. The most representative biomarkers of stage-III periodontitis in young women were bacteria of the phylum Bacteroidetes, its order, family and genera Bacteroidales, Prevotellaceae and Prevotella. The KEGG database revealed that the change in oral bacterial composition of young women with stage-III periodontitis may be related to protein processing in an endoplasmic reticulum pathway. Salubrinal (an endoplasmic reticulum stress regulator) controlled expression of Runx2, Col1a1, Ocn in mouse bone-marrow mesenchymal cells. Salubrinal administration showed that moderate endoplasmic reticulum stress inhibited alveolar bone loss in periodontitis induced by Prevotella intermedia lipopolysaccharide.ConclusionDifferences between periodontitis stages were noted and bacteria of Prevotella species were abundant in young women with stage-III periodontitis. This phenomenon was related to protein processing in an endoplasmic reticulum pathway.
Safety, efficacy, and clinical outcomes of transcatheter tricuspid valve replacement: One-year follow-up
Authors: Mao Yu , Li Lanlan , Liu Yang , Zhai Mengen , Ma Yanyan , Xu Chennian , Jin Ping , Yang Jian 
Published: December 2022

ObjectiveThe aim was to evaluate the safety and efficacy of TTVR in patients with severe TR at the 1-year follow-up.Materials and methodsThis project was a single-center, observational study. From September 2020 to May 2021, 15 patients with severe or extremely severe TR at high risk of traditional surgery were enrolled. All patients had preoperative imaging assessments to evaluate the tricuspid valve and the anatomy of the right heart. All patients were planned to treated with the LuX-Valve (Ningbo Jenscare Biotechnology, Ningbo, China). The LuX-Valve was implanted under the intraoperative guidance of TEE and X-ray fluoroscopy. Data were collected at baseline, before discharge, and at 30 days, 6 months, and 1 year postoperatively.ResultsThe LuX-Valves were successfully implanted in all 15 patients. TR was significantly reduced to ≤ 2 +. One patient died on postoperative day 12 of a pulmonary infection that was considered unrelated to the procedures or the devices. The remaining 14 patients (100.0%) reached the primary end point. One patient (7.1%) was rehospitalized during 1-year follow-up because of device thrombosis. The number of patients who survived at 1 year with New York Heart Association (NYHA) functional class II was higher than that before TTVR (11/14 vs. 0/15, P = 9.11 × 10–4). Patients with peripheral edema and ascites decreased from 100.0 to 46.7% at baseline to 28.6% and 14.3% at 1 year (P = 1.57 × 10–3 and 2.53 × 10–2).ConclusionTTVR is associated with RV remodeling, increased cardiac output, and improvement in NYHA functional class. Using the LuX-Valve for TTVR to treat patients with severe TR is a feasible and relatively safe method with reliable clinical results. Further studies are needed to determine long-term outcomes.
Characteristics and outcomes of percutaneous coronary interventions in patients with spontaneous coronary artery dissection. A study from the administrative minimum data set of the Spanish National Health System
Authors: Alfonso Fernando , Fernández-Pérez Cristina , del Prado Náyade , García-Guimaraes Marcos , Bernal José Luis , Bastante Teresa , del Val David , García-Márquez María , Elola Javier 
Published: December 2022

BackgroundCoronary revascularization in patients with spontaneous coronary artery dissection (SCAD) is challenging. Indications and results of percutaneous coronary interventions (PCI) in SCAD patients are not well established.AimTo assess indications and results of PCI in SCAD.MethodsThe minimum basic data set of the Spanish National Health System (years 2016−2019) was used to identify 804 episodes of acute myocardial infarction (AMI) and SCAD, with a crude in-hospital mortality rate of 3%. Of these, 368 (46.8%) patients were revascularized with PCI during admission whereas 436 (54.2%) were managed conservatively.ResultsRevascularization and in-hospital mortality rates both declined over the study period (p for trend both &lt; 0.05). SCAD patients treated with PCI were older, more frequently male, and had higher frequency of diabetes, ST-segment elevation AMI and cardiogenic shock, compared to patients managed conservatively. The crude in-hospital mortality rate was higher in patients treated with PCI (4.9% vs. 1.4%; p = 0.004). However, after adjusting by propensity score (223 pairs) the in-hospital mortality rate was similar in the two groups (Adj OR: 1.21; 95%CI: 0.30−1.57; p = 0.76). Readmissions at 30-days were higher in patients managed conservatively (7.1 vs. 1.6%, p &lt; 0.001) and this difference was maintained after propensity score adjustment (Adj average treatment effect: 2% vs. 12.2%; OR: 0.15; 95%CI: 0.04−0.45; p &lt; 0.001).ConclusionRevascularization is frequently used in unselected patients with AMI and SCAD but its use is declining. Patients with SCAD treated with PCI have a higher in-hospital mortality but this appears to be explained by their adverse baseline clinical characteristics.
Rapid response in relapsed follicular lymphoma with massive chylous ascites to anti-CD19 CAR T therapy using Piggy Bac: A case report
Authors: Zhang Yan , Lin Zhicai , Zhang Faliang , Chen Xiuxiu , Yang Yaping , Fu Xin , Li Zhong , Sun Yan , Qian Qijun 
Published: December 2022

CD19-directed chimeric antigen receptor (CAR) T cell therapy has been shown to achieve a considerably durable response in patients with refractory or relapsed B cell non-Hodgkin lymphomas, as seen from the results of Zuma-1, Zuma-5, and other clinical trials. Most of these CARs were generated by lentivirus or reverse adenovirus. It is rare to see CARs using non-viral vectors, such as Piggy Bac (pb), in treating lymphoma patients with active diseases. Generally, patients with a high tumor burden tend to have a higher rate of severe cytokine release syndrome (CRS) or neurological events as reported in the literature. Patients with symptomatic pleural effusions are excluded from the Zuma-1 trial because of the risk of severe CRS. We report here that a patient with relapsed follicular lymphoma with bulky disease and massive chylous ascites failed several lines of chemotherapy. After infusion of the CD19-directed pbCAR-T cells at 6 × 106 cells/kg, the patient had a rapid response and achieved a nearly complete metabolic remission on day 28. There was only grade 1 CRS, and no neurotoxicity occurred. The CAR-T cells reached a peak level on day 14 and spread into the ascites and expanded for 3 months. This might be the first case reported for pbCAR-T cells to treat relapsed follicular lymphoma directly. The long-term efficacy will be observed, and more patients be tested in the future.Clinical Trial Registrationhttps://ClinicalTrials.gov, identifier NCT05472610.
STXBP3 and GOT2 predict immunological activity in acute allograft rejection
Authors: Yao Qinfan , Wang Cuili , Wang Yucheng , Xiang Wenyu , Chen Yin , Zhou Qin , Chen Jianghua , Jiang Hong , Chen Dajin 
Published: December 2022

BackgroundAcute allograft rejection (AR) following renal transplantation contributes to chronic rejection and allograft dysfunction. The current diagnosis of AR remains dependent on renal allograft biopsy which cannot immediately detect renal allograft injury in the presence of AR. In this study, sensitive biomarkers for AR diagnosis were investigated and developed to protect renal function.MethodsWe analyzed pre- and postoperative data from five databases combined with our own data to identify the key differently expressed genes (DEGs). Furthermore, we performed a bioinformatics analysis to determine the immune characteristics of DEGs. The expression of key DEGs was further confirmed using the real-time quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), and immunohistochemical (IHC) staining in patients with AR. ROC curves analysis was used to estimate the performance of key DEGs in the early diagnosis of AR.ResultsWe identified glutamic-oxaloacetic transaminase 2 (GOT2) and syntaxin binding protein 3 (STXBP3) as key DEGs. The higher expression of STXBP3 and GOT2 in patients with AR was confirmed using RT-qPCR, ELISA, and IHC staining. ROC curve analysis also showed favorable values of STXBP3 and GOT2 for the diagnosis of early stage AR.ConclusionsSTXBP3 and GOT2 could reflect the immunological status of patients with AR and have strong potential for the diagnosis of early-stage AR.
Global research landscape and trends of lung cancer immunotherapy: A bibliometric analysis
Authors: Liu Yanhao , Cheng Xu , Han Xiaona , Cheng Xi , Jiang Shu , Lin Yaru , Zhang Zhen , Lu Linlin , Qu Baozhen , Chen Yuxian , Zhang Xiaotao 
Published: December 2022

​BackgroundImmunotherapy for lung cancer has been a hot research area for years. This bibliometric analysis aims to present the research trends on lung cancer immunotherapy.MethodOn 1 July, 2022, the authors identified 2,941 papers on lung cancer immunotherapy by the Web of Science and extracted their general information and the total number of citations. A bibliometric analysis was carried out to present the research landscape, demonstrate the research trends, and determine the most cited papers (top papers) as well as major journals on lung cancer immunotherapy. After that, recent research hotspots were analyzed based on the latest publications in major journals.ResultsThese 2,941 papers were cited a total of 122,467 times. “Nivolumab vs. docetaxel in advanced non–squamous non–small–cell lung cancer” published in 2015 by Borghaei H et al. was the most cited paper (5,854 citations). Among the journals, New England Journal of Medicine was most influential. Corresponding authors represented China took part in most articles (904) and papers with corresponding authors from the USA were most cited (139.46 citations per paper). Since 2015, anti–PD–(L)1 has become the hottest research area.ConclusionsThis bibliometric analysis comprehensively and quantitatively presents the research trends and hotspots based on thousands of publications, and further suggests future research directions. Moreover, the results can benefit researchers to select journals and find potential collaborators. This study can help researchers get a comprehensive impression of the research landscape, historical development, and recent hotspots in lung cancer immunotherapy and provide inspiration for further research.
Multiple sclerosis-disease modifying therapies affect humoral and T-cell response to mRNA COVID-19 vaccine
Authors: Dominelli Federica , Zingaropoli Maria Antonella , Tartaglia Matteo , Tortellini Eeva , Guardiani Mariasilvia , Perri Valentina , Pasculli Patrizia , Ciccone Federica , Malimpensa Leonardo , Baione Viola , Napoli Anna , Gaeta Aurelia , Lichtner Miriam , Conte Antonella , Mastroianni Claudio Maria , Ciardi Maria Rosa 
Published: December 2022

BackgroundThe mRNA vaccines help protect from COVID-19 severity, however multiple sclerosis (MS) disease modifying therapies (DMTs) might affect the development of humoral and T-cell specific response to vaccination.MethodsThe aim of the study was to evaluate humoral and specific T-cell response, as well as B-cell activation and survival factors, in people with MS (pwMS) under DMTs before (T0) and after two months (T1) from the third dose of vaccine, comparing the obtained findings to healthy donors (HD). All possible combinations of intracellular IFNγ, IL2 and TNFα T-cell production were evaluated, and T-cells were labelled “responding T-cells”, those cells that produced at least one of the three cytokines of interest, and “triple positive T-cells”, those cells that produced simultaneously all the three cytokines.ResultsThe cross-sectional evaluation showed no significant differences in anti-S antibody titers between pwMS and HD at both time-points. In pwMS, lower percentages of responding T-cells at T0 (CD4: p=0.0165; CD8: p=0.0022) and triple positive T-cells at both time-points compared to HD were observed (at T0, CD4: p=0.0007 and CD8: p=0.0703; at T1, CD4: p=0.0422 and CD8: p=0.0535). At T0, pwMS showed higher plasma levels of APRIL, BAFF and CD40L compared to HD (p&lt;0.0001, p&lt;0.0001 and p&lt;0.0001, respectively) and at T1, plasma levels of BAFF were still higher in pwMS compared to HD (p=0.0022).According to DMTs, at both T0 and T1, lower anti-S antibody titers in the depleting/sequestering-out compared to the enriching-in pwMS subgroup were found (p=0.0410 and p=0.0047, respectively) as well as lower percentages of responding CD4+ T-cells (CD4: p=0.0394 and p=0.0004, respectively). Moreover, the depleting/sequestering-out subgroup showed higher percentages of IFNγ-IL2-TNFα+ T-cells at both time-points, compared to the enriching-in subgroup in which a more heterogeneous cytokine profile was observed (at T0 CD4: p=0.0187; at T0 and T1 CD8: p =0.0007 and p =0.0077, respectively).ConclusionIn pwMS, humoral and T-cell response to vaccination seems to be influenced by the different DMTs. pwMS under depleting/sequestering-out treatment can mount cellular responses even in the presence of a low positive humoral response, although the cellular response seems qualitatively inferior compared to HD. An understanding of T-cell quality dynamic is needed to determine the best vaccination strategy and in general the capability of immune response in pwMS under different DMT.