bluuebunny/crossref_metadata_2025_split huggingface dataset:
A randomized controlled trial of the web-based drinking diary program for problem drinking in multi workplace settings
Authors: Sunami Takashi , So Ryuhei , Ishii Hironobu , Sadashima Eiji , Ueno Takefumi , Yuzuriha Takefumi , Monji Akira 
Published: January 2022

Abstract

Objectives
To assess the effectiveness of a web-based brief intervention (BI) program to record daily drinking among people with problem drinking in workplace settings.

Methods
A two-armed, parallel-group, randomized controlled trial were conducted at six workplaces in Japan. After obtaining written consent to participate in the study, workers with an Alcohol Use Disorders Identification Test (AUDIT) score of 8 or higher were randomly assigned into two groups. The participants allocated to the intervention group recorded their daily alcohol consumption for 4 weeks using the program, while those allocated to the control group received no intervention. Outcome measures included the amount of alcohol consumption in past 7 days using the Timeline Follow-Back method in the program at baseline, 8th week, and 12th week and written AUDIT score at baseline and 12th week.

Results
Hundred participants were assigned to either the intervention group (n = 50) or control group (n = 50). The results of two-way repeated measures ANOVA showed a statistically significant interaction between the group and the week factors in the two primary outcomes (number of alcohol-free days, total drinks) and secondary outcomes (AUDIT score) (p = .04, .02, and .03, respectively). The between-group effect sizes (Hedges' g; 95% CI) of the outcomes at 12th week were 0.53; 0.13–0.93 (total drinks), 0.44; 0.04–0.84 (AUDIT score), 0.43; 0.03–0.83 (number of alcohol-free days).

Conclusions
The web-based BI program for problem drinking was considered to be effective in reducing alcohol consumption and the AUDIT score in workplace settings.
Sounds of Synthesis: Acoustic Real‐Time Analysis of Laser‐Induced Graphene
Authors: Li Victor D. , Li John T. , Beckham Jacob L. , Chen Weiyin , Deng Bing , Luong Duy X. , Kittrell Carter , Tour James M. 
Published: January 2022

AbstractLaser‐induced graphene (LIG) can be patterned on a variety of substrates using a laser scriber/cutter. The quality and morphology of LIG is currently analyzed using Raman spectroscopy and electron microscopy. Here a method is presented to enable analysis of LIG in real‐time during the synthesis using a microphone attached to the laser writing head, followed by a simple acoustic signal processing scheme. It is demonstrated that it is possible to use the same energy input to simultaneously drive the conversion process and probe the formation of LIG in situ by applying Fourier and integral analyses, thus allowing rapid, high‐throughput determination of the local quality and morphology of the produced LIG.
Controlled Activation of TRPV1 Channels on Microglia to Boost Their Autophagy for Clearance of Alpha‐Synuclein and Enhance Therapy of Parkinson's Disease
Authors: Yuan Jiaxin , Liu Hanghang , Zhang Hao , Wang Tingting , Zheng Qing , Li Zhen 
Published: January 2022

AbstractParkinson's disease (PD) is characterized with accumulation of Lewy bodies with a major component of fibrillar alpha‐synuclein (α‐syn). Herein, boosting PD therapeutic efficacy by enhancing the autophagy of microglia to phagocytose and degrade α‐syn via controlled opening of their surface TRPV1 channels with rationally designed photothermal nanoagent is reported. The Cu2−xSe‐anti‐TRPV1 nanoparticles (CS‐AT NPs) are fabricated to target the microglia and open their surface TRPV1 channels under the second near infrared (NIR‐II) laser irradiation to cause influx of Ca2+ to activate ATG5 and Ca2+/CaMKK2/AMPK/mTOR signaling pathway, which promote phagocytosis and degradation of α‐syn. The CS‐AT NPs are efficiently delivered by focused ultrasound into striatum of PD mice with high expression of TRPV1 receptors. The athletic ability of PD mice treated by CS‐AT NPs and NIR‐II irradiation is significantly improved due to the phagocytotic clearance of α‐syn by microglia with enhanced autophagy. The enzyme tyrosine hydroxylase, ionized calcium binding adapter protein 1, glial fibrillary acidic protein, and pSer129‐α‐syn (p‐α‐syn) of treated PD mice are almost recovered to the normal levels of healthy mice. This study provides insights into the activation of microglial autophagy by targeting surface ion channels to improve the treatment of PD and other neurodegenerative diseases.
Highly Efficient and Flexible Scintillation Screen Based on Manganese (II) Activated 2D Perovskite for Planar and Nonplanar High‐Resolution X‐Ray Imaging
Authors: Shao Wenyi , Wang Xiang , Zhang Zhenzhong , Huang Jie , Han Zhongyuan , Pi Shuai , Xu Qiang , Zhang Xiaodong , Xia Xiaochuan , Liang Hongwei 
Published: January 2022

AbstractX‐ray imaging technology covers a wide range of applications in the medical, industrial, and scientific research fields. Highly sensitive, flexible, and cost‐effective scintillation screens are of great importance for X‐ray imaging applications. In this paper, manganese (II) activated 2D butylammonium lead bromide perovskite, namely BA2PbBr4:Mn (II), is demonstrated as a highly efficient and low‐cost X‐ray scintillator. The appropriate amount of Mn (II) dopant can act as an activator for achieving the optimization of luminescence performance through efficient energy transfer. This produces a large Stokes shift thus almost eliminates the effect of self‐absorption. As a result, the optimal BA2PbBr4:10% Mn (II) perovskite scintillator demonstrates an ultrahigh sensitivity for X‐ray, with a high X‐ray scintillation light yield of up to 85 ± 5 photons keV−1 and low detection limits of 16 nGyair s−1 at the signal‐to‐noise ratio (SNR) of 3. Well‐resolved X‐ray images of several planar and nonplanar objects are obtained using a flexible large‐dimensional scintillation screen fabricated by blade‐coating strategy, featuring high spatial resolution of 10.7 lp mm−1 at modulation transfer function = 0.2. These results indicate that Mn (II) activated BA2PbBr4 perovskite with remarkable scintillation performances can serve as a highly promising scintillator for commercial high‐resolution X‐ray imaging applications.
UNC5C Receptor Proteolytic Cleavage by Active AEP Promotes Dopaminergic Neuronal Degeneration in Parkinson's Disease
Authors: Chen Guiqin , Ahn Eun Hee , Kang Seong Su , Xia Yiyuan , Liu Xia , Zhang Zhaohui , Ye Keqiang 
Published: January 2022

AbstractNetrin‐1 is a chemotropic cue mediating axon growth and neural migration in neuronal development, and its receptors deletion in colorectal cancer and UNC5s act as dependence receptors regulating neuronal apoptosis. Asparagine endopeptidase (AEP) is an age‐dependent protease that cuts human alpha‐synuclein (α‐Syn) at N103 and triggers its aggregation and neurotoxicity. In the current study, it is reported that UNC5C receptor is cleaved by AEP in Parkinson's disease (PD) and facilitates dopaminergic neuronal loss. UNC5C is truncated by active AEP in human α‐SNCA transgenic mice in an age‐dependent manner or induced by neurotoxin rotenone. Moreover, UNC5C is fragmented by AEP in PD brains, inversely correlated with reduced netrin‐1 levels. Netrin‐1 deprivation in primary cultures induces AEP and caspase‐3 activation, triggering UNC5C proteolytic fragmentation and enhancing neuronal loss. Noticeably, blocking UNC5C cleavage by AEP attenuates netrin‐1 deprivation‐elicited neuronal death and motor disorders in netrin flox/flox mice. Overexpression of AEP‐truncated UNC5C intracellular fragment strongly elicits α‐Syn aggregation and dopaminergic loss, locomotor deficits in α‐SNCA transgenic mice. Hence, the findings demonstrate that netrin‐1 reduction and UNC5C truncation by AEP contribute to PD pathogenesis.
Synthesis of Substituted Pyrano[3,4‐b]Quinolines by Silver‐Catalyzed Regioselective Intramolecular Cyclization of 3‐Alkynylquinoline Aldehydes
Authors: Vani Damera , Chahal Kapil , Preethi Pagilla , Balasubramanian Sridhar , Rajender Reddy Kallu 
Published: January 2022

AbstractA novel approach for the synthesis of regioselective pyrano[3,4‐b]quinolines from 3‐alkynylquinoline aldehydes with alcohols as nucleophiles has been elucidated. This protocol has shown high generality of functional group and provides the pyrano‐annulated products good to excellent yields. This methodology was adopted a new synthetic strategy to develop the 3‐alkynylquinolinealdehydes by using methanol as a C1 source.
Synthesis and in vitro biological evaluation of novel water‐soluble porphyrin complexes for cancer photodynamic therapy
Authors: Hou Bingjie , Zhang Wenyuan , Li Cuili , Sun Xueqin , Feng Xiaoxia , Liu Jiacheng 
Published: January 2022

AbstractIn this study, we report the synthesis and biological evaluation of four novel cationic porphyrins (named as P1, P2, ZnP2, and CuP2) with potential as a photodynamic therapeutic agent. MTT experiments proved that P1, P2, and ZnP2 have high light toxicity and low dark toxicity for A549, H‐1975, and HepG2 cell lines. In addition, due to the specific targeting of porphyrin, there was only a weak toxicity to Hs 578Bst normal breast cancer cells. The study further confirmed the intracellular generation of reactive oxygen species (ROS) under light irradiation and ZnP2 could be effectively internalized by cells and also indicated that the photodynamic therapy effect achieved by ZnP2 and light irradiation can cause significant cell apoptosis. High relative singlet oxygen quantum yield and excellent phototoxicity ensure the optimal photosensitizer ZnP2 becomes potential candidates for photodynamic therapy drugs. Our results disclosed that the cationic porphyrin complexes used photodynamic therapy for the development of new and useful metal anticancer photosensitizer provide a strong platform.
Specializations of somatosensory innervation in the skin of humpback whales (Megaptera novaeangliae)
Authors: Eldridge Sherri A. , Mortazavi Farzad , Rice Frank L. , Ketten Darlene R. , Wiley David N. , Lyman Ed , Reidenberg Joy S. , Hanke Frederike D. , DeVreese Steffen , Strobel Sarah McKay , Rosene Douglas L. 
Published: January 2022

AbstractCetacean behavior and life history imply a role for somatosensory detection of critical signals unique to their marine environment. As the sensory anatomy of cetacean glabrous skin has not been fully explored, skin biopsy samples of the flank skin of humpback whales were prepared for general histological and immunohistochemical (IHC) analyses of innervation in this study. Histology revealed an exceptionally thick epidermis interdigitated by numerous, closely spaced long, thin diameter penicillate dermal papillae (PDP). The dermis had a stratified organization including a deep neural plexus (DNP) stratum intermingled with small arteries that was the source of intermingled nerves and arterioles forming a more superficial subepidermal neural plexus (SNP) stratum. The patterns of nerves branching through the DNP and SNP that distribute extensive innervation to arteries and arterioles and to the upper dermis and PDP provide a dense innervation associated through the whole epidermis. Some NF‐H+ fibers terminated at the base of the epidermis and as encapsulated endings in dermal papillae similar to Merkel innervation and encapsulated endings seen in terrestrial mammals. However, unlike in all mammalian species assessed to date, an unusual acellular gap was present between the perineural sheaths and the central core of axons in all the cutaneous nerves perhaps as mechanism to prevent high hydrostatic pressure from compressing and interfering with axonal conductance. Altogether the whale skin has an exceptionally dense low‐threshold mechanosensory system innervation most likely adapted for sensing hydrodynamic stimuli, as well as nerves that can likely withstand high pressure experienced during deep dives.
Improving the effectiveness of voice search systems through partial query modification
Authors: Sa Ning , Yuan Xiaojun (Jenny) 
Published: January 2022

AbstractThis paper addresses the importance of improving the effectiveness of voice search systems through partial query modification. A user‐centered experiment was designed to compare the effectiveness of an experimental system using partial query modification feature to a baseline system in which users could issue complete queries only, with 32 participants each searching on eight different tasks. The results indicate that the participants spent significantly more time preparing the modification but significantly less time speaking the modification by using the experimental system than by using the baseline system. The participants found that the experimental system (a) was more effective, (b) gave them more control, (c) was easier for the search tasks, and (d) saved them time than the baseline system. The results contribute to improving future voice search system design and benefiting the research community in general. System implications and future work were discussed.
Combining recombinase polymerase amplification and DNA‐templated reaction for SARS‐CoV‐2 sensing with dual fluorescence and lateral flow assay output
Authors: Farrera‐Soler Lluc , Gonse Arthur , Kim Ki Tae , Barluenga Sofia , Winssinger Nicolas 
Published: January 2022

AbstractThe early phase of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) pandemic was exacerbated by a diagnostic challenge of unprecedented magnitude. In the absence of effective therapeutics or vaccines, breaking the chain of transmission through early disease detection and patient isolation was the only means to control the growing pandemic. While polymerase chain reaction (PCR)‐based methods and rapid‐antigen tests rose to the occasion, the analytical challenge of rapid and sequence‐specific nucleic acid‐sensing at a point‐of‐care or home setting stimulated intense developments. Herein we report a method that combines recombinase polymerase amplification and a DNA‐templated reaction to achieve a dual readout with either fluorescence (microtiter plate) or naked eye (lateral flow assay: LFA) detection. The nucleic acid templated reaction is based on an SNAr that simultaneously transfers biotin from one Peptide Nucleic Acid (PNA) strand to another PNA strand, enabling LFA detection while uncaging a coumarin for fluorescence readout. This methodology has been applied to the detection of a DNA or RNA sequence uniquely attributed to the SARS‐CoV‐2.
A Capstone Laboratory theme investigating properties of non‐steroidal anti‐inflammatory drugs and their solubilization by cationic surfactant micelles
Authors: Roach Jimmy D. , Nauman Areej , Mahmud Shamin , Chaari Ali , Bondaruk Mandy 
Published: January 2022

AbstractResearch experience is becoming an increasingly crucial element of today's undergraduate science curriculum. In recent years, the establishment of the Course‐Based Undergraduate Research Experience Network (CUREnet) has provided a framework for the development of courses aiming to cultivate students' proficiency in undergraduate research techniques while fostering scientific curiosity in a laboratory setting. Capstone Laboratory is one such course that was designed to provide second‐year pre‐medical students at Weill Cornell Medicine‐Qatar with exposure to laboratory techniques to develop their repertoire of research skills. For the past 3 years, Capstone Laboratory has incorporated a project‐based learning component involving non‐steroidal anti‐inflammatory drugs (NSAIDs). The research element of the NSAID‐surfactant theme investigated the partitioning of various drugs into cationic surfactant micelles. Structural elements and enzyme inhibition characteristics of NSAIDs were also investigated, while integrating principles of biochemistry, general chemistry, and experimental organic chemistry. By studying drug properties in these contexts, students integrate their learning and develop skills needed for the study and practice of medicine.
Characterization of m6A regulator‐mediated methylation modification patterns and tumor microenvironment infiltration in acute myeloid leukemia
Authors: Han Shiyu , Qi Jiaqian , Fang Kun , Wang Hong , Tang Yaqiong , Wu Depei , Han Yue 
Published: January 2022

AbstractBackgroundPrevious studies have confirmed the existence of epigenetic regulation of immune responses in acute myeloid leukemia. However, the potential role of RNA N6‐methyladenosine (m6A) remodeling in tumor microenvironment (TME) infiltration remains unclear.Methods and Materialsm6A patterns of 469 AML patients (420 of which provided survival data) based on 18 m6A regulators were systematically evaluated. Based on the expression of 18 m6A regulators, unsupervised agglomerative cluster analysis was applied to recognize the various m6A modification types and to classify patients. We linked these patterns to TME infiltration characteristics and identified three distinct populations of m6A modifications.ResultsThese three TME cell infiltration patterns are characterized by a high degree of concordance with the three tumor immunophenotypes, which include immunoinflammatory, immunorejection, and immune inert patterns. We showed that assessment of m6A modification patterns within individually neoplasms can forecast the stage of neoplasmic inflammation, TME basal activity, subtype, hereditary mutation, and clinical patient prognosis. Limited low m6Ascore, featuring increased mutational load and immune activation, indicates an inflammatory phenotype of TME with a 5‐year survival rate at 14.4% compared to the high‐m6Ascore group (40.9%).ConclusionsData from two different cohorts demonstrated that a higher m6Ascore showed a marked therapeutic superiority as well as clinical advantage. Assessing m6A modification patterns in AML patients could improve our knowledge of the TME infiltrative profile as well as directing effective immunotherapeutic approaches.
Myelin and lymphocyte protein serves as a prognostic biomarker and is closely associated with the tumor microenvironment in the nephroblastoma
Authors: Su Cheng , Huang Rongzhi , Yu Zhenyuan , Zheng Jie , Liu Fengling , Liang Haiqi , Mo Zengnan 
Published: January 2022

AbstractNephroblastoma, also known as Wilms' tumor (WT), is the most common renal tumor that occurs in children. Although the efficacy of treatment has been significantly improved by a series of comprehensive treatments, some patients still have poor prognosis. Myelin and lymphocyte (MAL) protein, a highly hydrophobic integrated membrane‐bound protein, has been implicated in many tumors and is also closely linked to kidney development. However, the relationship between MAL and WT has not yet been elucidated. Therefore, we attempted to evaluate the feasibility of MAL as a promising prognosis factor for WT. The differential expression of MAL was investigated using TARGET database and was verified using the Gene Expression Omnibus database and real‐time quantitative PCR. The prognostic ability of MAL was determined using Kaplan–Meier and Cox regression analyses. Pearson correlation analysis was applied to explore the relationship between MAL expression and methylation sites. The ESTIMATE and CIBERSORT algorithms showed that MAL expression was associated with the WT tumor microenvironment. Gene Set Enrichment Analysis (GSEA) indicated that multiple signaling pathways closely associated with tumorigenesis were differentially enriched between the high‐ and low‐MAL groups. In conclusion, our study comprehensively explored the potential of MAL as a prognosis factor for WT. Meanwhile, we also demonstrated that MAL, as a prognostic factor for WT, may be closely related to the tumor microenvironment.
Protective Effects of Chrysin against Cyclophosphamide‐Induced Cardiotoxicity in Rats: A Biochemical and Histopathological Approach
Authors: Ye Bin , Ling Wenchao , Wang Yinhua , Jaisi Amit , Olatunji Opeyemi Joshua 
Published: January 2022

AbstractMounting evidences have indicated that cyclophosphamide (CyC)a potent anticancer and cytotoxic agent is associated with various organ and systemic toxicities and the cytotoxic effects observed after administration of CyC still challenges its clinical use. Chrysin (Chy) is a dietary flavonoid with prevailing antioxidant and anti‐inflammatory properties. This study evaluated the protective properties of Chy against CyC‐induced cardiotoxicity in rats. The animals were orally treated with Chy (25 and 50 mg/kg/day) for 35 days and exposed to CyC (i. p., 100 mg/kg) once a week for four weeks. The results indicated that CyC caused significant cardiotoxicity as manifested by notable increases in heart weight, cardiac function biomarkers such as lactate dehydrogenase (LDH), creatine kinase‐MB (CK‐MB), troponin T and aspartate transaminase (AST). In addition, cardiac malondialdehyde (MDA), tumor necrosis factor alpha (TNF‐α), interleukin 1β (IL1 β) and interleukin 6 (IL‐6) levels were considerably increased. Meanwhile, cardiac antioxidant enzymes activities such as superoxide dismutase (SOD) and catalase (CAT), as well as glutathione (GSH) level were suppressed, while H&E stained histopathological assessment showed marked alterations in cardiac tissues. CyC also significantly lowered red blood cells (RBC) and white blood cells (WBC) parameters, whereas treatment with Chy significantly restored the altered biochemical and histopathological features. Conclusively, aforementioned results inferred that Chy offered cardioprotective potentials against CyC‐induced cardiotoxicity which may be due to its antioxidant, and anti‐inflammatory properties.
Androgen downregulates desmocollin‐2 in association with induction of mesenchymal transition of breast MDA‐MB‐453 cancer cells
Authors: Ahram Mamoun , Abdullah Mohammad S. , Mustafa Shahed A. , Alsafadi Dana B. , Battah Abdelkader H. 
Published: January 2022

AbstractDesmosomes are cellular structures that are critical in cell–cell adhesion and in maintaining tissue architecture. Changes in the expression of desmocollin‐2 (DSC2) have been noted during tumor progression into an invasive phenotype and as cells undergo epithelial‐mesenchymal transition. We have previously reported that breast MDA‐MB‐453 cancer cells, a luminal androgen receptor (AR) model of triple‐negative breast cancer, acquire mesenchymal features when treated with the AR agonist, dihydrotestosterone (DHT). We have therefore investigated androgen regulation of the expression and cellular localization of DSC2 in MDA‐MB‐453 cells. Treatment of the cells with DHT resulted in a dose‐dependent reduction in DSC2 protein levels and dispersion of its membrane localization concomitant with AR‐ and β‐catenin‐mediated mesenchymal transition of cells. A significant correlation was revealed between decreased expression of AR and increased expression of DSC2 in patient samples. In addition, whereas lower expression of AR was associated with a reduced overall and recurrence‐free survival of breast cancer patients, higher expression of DSC2 was found in invasive breast tumors than in normal breast cells and was correlated with lower patient survival. Upon knocking down DSC2, the cells became elongated, mesenchymal‐like, and slightly, but insignificantly, more migratory. The addition of DHT further stimulated cell elongation and migration. DSC2 siRNA‐transfected cells reverted to a normal epithelial morphology upon inhibition of β‐catenin. These results highlight the role of DSC2 in maintaining the epithelial morphology of MDA‐MB‐453 cells and the negative regulation of the desmosomal protein by DHT during stimulation of the androgen‐induced, β‐catenin‐mediated mesenchymal transition of the cells.