bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pensamiento complejo, ética planetaria y transformación social en la formación del profesorados
Authors: López-Calva Juan Martín 
Published: February 2022

Introducción: La crisis que vive el mundo actual demanda la atención a la formación ética de las nuevas generaciones. Un elemento indispensable para atenderla es la formación ética de los docentes. Objetivo: Presentar los resultados de una investigación exploratoria que analizó la opinión de profesores y expertos en programas de capacitación docente sobre la formación ética del profesorado desde el bucle: pensamiento complejo-ética planetaria-transformación social. Método: Se encuestó a un grupo de 22 expertos en formación del profesorado y a 222 docentes de distintos niveles educativos, se les aplicó un cuestionario exploratorio sobre la formación ética del profesorado, construido con una escala Likert de siete opciones. Se presentan descriptiva y comparativamente los resultados de las preguntas relativas al bucle descrito en el objetivo, que es parte de la propuesta teórica construida. Resultados: muestran que no hay suficiente atención a la formación ética del profesorado desde las dimensiones: pensamiento complejo-ética planetaria- transformación social. Discusión: la opinión de los profesores es ligeramente más positiva que la de los expertos, aunque ambos grupos muestran la insuficiencia de la formación de los docentes en estos rubros. Al ser un estudio exploratorio, aporta una primera mirada comparativa. Será necesario complementarlo con un estudio cualitativo.
АНАЛИЗ РАСПРОСТРАНЕННОСТИ И СТРУКТУРЫ БЕССИМПТОМНОГО НОСИТЕЛЬСТВА SARS-COV2 У ПОДРОСТКОВ И ВЗРОСЛЫХ В ПЕРИОД ЭПИДЕМИЧЕСКОГО ПОДЪЕМА ЗАБОЛЕВАЕМОСТИ COVID-19 В ЯНВАРЕ 2022 г.
Authors: Башкетова Наталия , Фридман Роман , Катаева Ирина , Шапарь Александр , Костина Марина , Журавлев Андрей , Пшеничная Наталья 
Published: February 2022

РЕЗЮМЕ
Цель: провести анализ распространенности и структуры бессимптомного носительства SARS-CoV2 у студентов и преподавателей колледжей г.Санкт-Петербурга в период подъема заболеваемости COVID-19 в январе 2022.
Материалы и методы. В период с 25.01.2022 по 27.01.2022 проведен выборочный отбор проб из носоглотки и ротоглотки у 3 886 из 8 350 студентов и преподавателей 4-х колледжей, расположенных в разных районах г. Санкт-Петербурга. Обследуемый контингент не имел признаков ОРВИ и других симптомов, не исключающих COVID-19. Пробы исследовались ме-тодом ПЦР на РНК SARS-CoV2. Положительные пробы тестировали на мутации к вариантам «Омикрон» и «Дельта» с использованием лабораторной ПЦР-методики, разработанной ФБУН «НИИ эпидемиологии и микробиологии имени Пастера» Роспотребнадзора. Лабораторные исследования проводились на базе ФБУЗ «Центр гигиены и эпидемиологии в городе Санкт-Петербурге».
Результаты. Продемонстрирована высокая частота выявления бессимптомных позитивных по SARS-CoV2 лиц среди студентов и преподавателей образовательных учреждений г. Санкт-Петербурга в период подъема заболеваемости COVID-19, вызванного преобладающим вариантом «Омикрон». В среднем, у подростков в возрасте 14-17 лет частота как положительных проб (20,6%), так и удельный вес варианта «Омикрон» в позитивных пробах (51,7%) были значимо выше, чем у лиц в возрасте 18 лет и старше ( 10,1% и 33,1%, соответственно). Указанная закономерность прослеживалась во всех анализируемых образовательных учреждениях. Подростки в возрасте 14-17 лет статистически значимо, в 2,3 раза чаще, являлись бессимптомными носителями вируса, чем взрослые. Кроме того, подростки 14-17 лет значимо чаще (в 3,3 раза) были инфицированы вариантом “Омикрон”, по сравнению с когортой исследуемых лиц в возрасте 18 лет и старше. Отмечена значимая прямая корреляционная связь между увеличением числа тестируемых и вероятностью выявления бессимптомных носителей, в том числе, инфицированных вариантом «Омикрон».
Выводы: в период подъема заболеваемости COVID-19 в январе 2022 года отмечается высокая распространенность бессимптомного носительства вируса SARS-CoV2 среди студен-тов и преподавателей образовательных учреждений, расположенных в разных районах города. Подростки более чем в 2 раза чаще являются носителями SARS-CoV2 и более чем в 3 раза чаще носителями варианта «Омикрон», по сравнению со взрослыми. Это демонстрирует нецелесообразность обследования и изоляции контактных лиц, из числа учащихся и преподавателей образовательных учреждений, не имеющих клинических проявлений инфекции. Cохраняет актуальность обследование и изоляция лиц с симптомами заболевания, перевод лиц из групп риска на дистанционный режим работы или обучения. а также усиленный контроль за соблюдением мер неспецифической профилактики во всех общественных учреждениях.
REBOA and its effect on hemodynamics and cerebral blood flow as measured by CTP in a large animal model of raised intracranial pressure and hemorrhagic shock.
Authors: Edwards Joseph , Elansary Noha , Stonko David P , Abdou Hossam , Lang Eric , Treffalls Rebecca N , Savidge Samuel G , Morrison Jonathan J 
Published: February 2022

Abstract
Hemorrhage is among the leading causes of preventable deaths in trauma. Further traumatic brain injury (TBI) often present in a polytrauma patient, and is known to worsen outcomes when present with non compressible torso hemorrhage (NCTH). Resuscitative endovascular balloon occlusion of the aorta (REBOA) has been developed as a means of hemorrhage control and afterload increase during a state of hemorrhagic shock, but its use in patients with both hemorrhagic shock and concomitant TBI is controversial, as the potential for ischemia reperfusion injury and acute hypertension may cause intracranial lesion progression. To date, there is limited data on how REBOA alters hemodynamics in a model of hemorrhagic shock and brain injury resulting in raised intracranial pressure. The goal of the current study is to define how full REBOA and partial REBOA alter systemic and cerebral hemodynamics in a porcine model of TBI with raised ICP with concomitant hemorrhagic shock. This will be examined throughout the intervention and for 3 hours of resuscitation. It will be evaluated with hemodynamic, laboratory, and radiographic assessments.
Localization of cortical TMS effects and accurate motor threshold determination
Authors: Weise Konstantin , Numssen Ole , Kalloch Benjamin , Zier Anna Leah , Thielscher Axel , Hartwigsen Gesa , Knösche Thomas R. 
Published: February 2022

Abstract
We describe a routine to precisely localize cortical muscle representations within the primary motor cortex with transcranial magnetic stimulation (TMS). This allows for an accurate and experimenter-independent determination of motor thresholds and enables TMS dosing based on individual field thresholds. Motor evoked potentials (MEPs) for different coil positions/orientations are combined with electric field modelling. Compared to previous approaches this routine exploits the causal nature of neuronal activation functions to pinpoint MEPs to their cortical origin.After constructing an individual head model, MEPs are recorded, and the induced electric fields computed. Subsequently, the cortical muscle representations are automatically determined by relating the induced fields to MEPs. Finally, the coil position to optimally assess the motor threshold is identified.The protocol requires one day of preparation, one TMS session (2h), 12h post-processing, and a second TMS session (0.5h). A basic level of expertise and standard TMS neuronavigation equipment suffices.
Analyzing the Breakthrough of COVID-19 infection Incidence Rate, Severity and Mortality among the COVID-19 Vaccinated Population of West Bengal, India
Authors: Mukherjee Dattatreya , Dhara Bikram , Basu Riddhi , Rozario Prapti Victoria , Dudhraj Vibhor , Mukhopadhyay Debraj , Roy Sayan , Roy Ishita , Mitra Arup Kumar 
Published: February 2022

Abstract
World is facing the pandemicity of COVID 19. There are various types of mutations occurring in the genomics of SARS-Cov 2. COVID 19 vaccination has been already started. Common Vaccines are Pfizer, Moderna, Jassen, Sinovac, Covidshield, Covaxin, Sputnik V. In India, Covidshield and Covaxin have been majorly administered. Some people have taken Sputnik V also. We have proposed a Protocol of Analyzing the Breakthrough of COVID 19 infection Incidence Rate, Severity and Mortality among the COVID 19 Vaccinated Population of West Bengal, India and a State of the Art Systematic Review on using mask as a preventive measure against COVID 19 pandemic. This study will help to analyze the vaccine effectiveness and duration of effectiveness among the population of West Bengal. The systematic analysis of using mask will give a broader picture towards protective measures against COVID 19
The Expression of miR-153-3p Regulates the Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells by Targeting RUNX2
Authors: Ma Rong , Miao Junyi , Fang Shuwen , Ji Mufan , yi Xin , Zhang Xinhua 
Published: February 2022

Abstract
Background
Runt-related transcription factor 2 (RUNX2) plays a key role in the osteogenic differentiation. The study aimed to explore the effect of miR-153-3p on the osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hBMSCs) and confirm its targeting effect on RUNX2.
Results
During the osteogenic differentiation of hBMSCs, the expression of miR-153-3p decreased at 1 d and reached a low peak at 3 d. After miR-153-3p mimic or inhibitor transfection, miRNA-153-3p expression level of hBMSCs dramatically increased or decreased, but the cell viability and proliferation ability were not notably changed. After miR-153-3p inhibitor transfection, the osteogenic differentiation was notably promoted which was confirmed with Alizarin red staining, alkaline phosphatase (ALP) activity and Western blot for RUNX2 and Collagen I, while miR-153-3p mimic transfection, the osteogenic differentiation was notably suppressed. The result of luciferase reporter gene experiment verified that RUNX2 was the target gene of miR-153-3p.
Conclusion
The results of this study indicate that the expression of miR-153-3p regulates the osteogenic differentiation of hBMSCs by targeting RUNX2.
Changes In R-wave Amplitude At Implantation Are Associated With Gender and Postures: Observations From The Confirm Rx™ Body Posture and Physical Activity Study
Authors: Swale Matthew , Paul Vincent , Delacroix Sinny , Young Glenn , McSpadden Luke , Ryu Kyungmoo , Fiore David Di , Santos Maria , Tan Isabel , Conradie Andre , Duong MyNgan , Schwarz Nisha , Worthley Stephen , Pavia Stephen 
Published: February 2022

Abstract
Background: Insertable cardiac monitors (ICMs) are small subcutaneously implanted devices that detect changes in R-wave amplitudes (RWAs), effective in arrhythmia-monitoring. Although ICMs have proven to be immensely successful, there is a growing need to sustain and increase efficacy in detection rates irrespective of changes in body postures and activities. Methods: RWAs were measured in 15 separate postures, including supine, lying on the right-side (RS) or left-side (LS) and sitting, immediately after implantation of Confirm Rx™ ICM in 99 patients. Results: The patients (53 females and 46 males, mean ages 66.62±14.7 and 66.40±12.25 years, respectively) had attenuated RWAs in RS, LS and sitting by ~26.4%, ~27.8% and ~21.2% respectively, compared to supine. Gender-based analysis indicated RWAs in RS (0.32mV (0.09-1.03mV), p<0.0001) and LS (0.37mV (0.11-1.03mV), p=0.004) to be significantly attenuated compared to supine (0.52mV(0.20-1.03mV) for female participants. Similar attenuation was not evident for male participants. Further, parasternally oriented ICMs (n=44), attenuated RWAs in RS (0.37mV(0.09-1.03mV), p=0.05) and LS (0.34mV (0.11-1.03mV), p=0.02) compared to supine (0.48mV (0.09-1.03mV). Similar differences were not observed in participants with ICMs in the 45°-relative-to-sternum (n=46) orientation. When assessing the combined effect of gender and ICM orientation, female participants demonstrated plausible attenuation in RWAs for RS and LS postures compared to supine, an effect not observed in male participants.Conclusion: This is the first known study to evaluate effects of body postures and activities on RWAs immediately post-implantation with an ICM. Future work assessing the cause of gender-based differences in RWAs may be critical.Trial registration: Clinical Trials, NCT03803969. Registered 15 January 2019 – Retrospectively registered, https://clinicaltrials.gov/NCT03803969
Consensus on a Standardised Treatment Pathway Algorithm for Lumbar Spinal Stenosis: an International Delphi Study
Authors: Comer Christine , DC Carlo Ammendolia , Battié Michele C. , Bussières André , Fairbank Jeremy , Haig Andrew , Melloh Markus , Redmond Anthony , Schneider Michael J. , Standaert Christopher J. , Tomkins-Lane Christy , Williamson Esther , Wong Arnold YL. 
Published: February 2022

Abstract
Background Lumbar spinal stenosis (LSS) is a common degenerative spinal condition in older adults associated with disability, diminished quality of life, and substantial healthcare costs. Individual symptoms and needs vary. With sparse and sometimes inconsistent evidence to guide clinical decision-making, variable clinical care may lead to unsatisfactory patient outcomes and inefficient use of healthcare resources.Methods A three-phase modified Delphi study comprising four consensus rounds was conducted on behalf of the International Taskforce for the Diagnosis and Management of LSS to develop a treatment algorithm based on multi-professional international expert consensus. Participants with expertise in the assessment and management of people with LSS were invited using an international distribution process used for two previous Delphi studies led by the Taskforce. Separate treatment pathways for patients with different symptom types and severity were developed and incorporated into a proposed treatment algorithm through consensus rounds 1 to 3. Agreement with the proposed algorithm was evaluated in the final consensus round.Results The final algorithm combines stratified and stepped approaches. When indicated, immediate investigation and surgery is advocated. Otherwise, a stepped approach is suggested when self-directed care is unsatisfactory. This starts with tailored rehabilitation, then more complex multidisciplinary care, investigations and surgery options if needed. Treatment options in each step depend on clinical phenotype and symptom severity. Treatment response guides pathway entrance and exit points. Of 397 study participants, 86% rated their agreement >4 for the proposed algorithm on a 0-6 scale, of which 22% completely agreed. Only 7% disagreed. Over 70% of participants felt that the algorithm would be useful for clinicians in public healthcare (both primary care and specialist settings) and in private healthcare settings, and that a simplified version would help patients in shared decision-making.Conclusions International and multi-professional agreement was achieved for a proposed LSS treatment algorithm developed through expert consensus. The algorithm advocates different pathway options depending on clinical indications. It is not intended as a treatment protocol and will require evaluation against current care for clinical and cost-effectiveness. It may, however, serve as a clinical guide until evidence is sufficient to inform a fully stratified care model.
Quantitatively determine the dominant driving factors of the spatial-temporal changes of vegetation-impacts of global change and human activity
Authors: wang shijun , chang ping , wang ning , wen jin , zhang ke , yuan kun , yang jun 
Published: February 2022

Abstract
The differences of spatial-temporal distribution patterns and dominant driving factors of vegetation evolution in different regions and different historical periods were not clearly. Therefore, this study introduced the gravity center model and geodetector to analyze the spatial-temporal change characteristics and dominant driving factors of vegetation NDVI in China during 1981-2019 from the perspective of geographic divisions. Results showed that: (1) During 1981-2019, the average vegetation coverage in China, as a whole, showed an increasing trend and zones with obviously increasing vegetation NDVI were mainly distributed in the middle reaches of the Yellow River basin and the upper reaches of the Yangtze River. (2) During 1981-2019, the gravity centers of vegetation NDVI, nationally, were mainly concentrated in Yan'an City and Tongchuan City, showing a southward migration trend as a whole, which indicates that the increment and growth rates of the southern were greater than those of the northern part. (3) The growth of vegetation in southern China was primarily affected by temperature, while that of northern China was largely influenced by precipitation. (4) Specific factors, including land use, precipitation and soil types exerted significant influences on the vegetation NDVI, and their interaction could enhance the influence of single factor.
Rapidly Identifying New Coronavirus Mutations of Potential Concern in the Omicron Variant Using an Unsupervised Learning Strategy
Authors: Zhao Lue Ping , Lybrand Terry , Gilbert Peter , Payne Thomas H. , Pyo Chul-Woo , Geraghty Daniel , Jerome Keith 
Published: February 2022

Abstract
Extensive mutations in the Omicron spike protein appear to accelerate the transmission of SARS-CoV-2, and rapid infections increase the odds that additional mutants will emerge. To build an investigative framework, we have applied an unsupervised machine learning approach to 4296 Omicron viral genomes collected and deposited to GISAID as of December 14, 2021, and have identified a core haplotype of 28 polymutants (A67V, T95I, G339D, R346K, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, K796Y, N856K, Q954H, N69K, L981F) in the spike protein and a separate core haplotype of 17 polymutants in non-spike genes: (K38, A1892) in nsp3, T492 in nsp4, (P132, V247, T280, S284) in 3C-like proteinase, I189 in nsp6, P323 in RNA-dependent RNA polymerase, I42 in Exonuclease, T9 in envelope protein, (D3, Q19, A63) in membrane glycoprotein, and (P13, R203, G204) in nucleocapsid phosphoprotein. Using these core haplotypes as reference, we have identified four newly emerging polymutants (R346, A701, I1081, N1192) in the spike protein (p-value=9.37*10-4, 1.0*10-15, 4.76*10-7 and 1.56*10-4, respectively), and five additional polymutants in non-spike genes (D343G in nucleocapsid phosphoprotein, V1069I in nsp3, V94A in nsp4, F694Y in the RNA-dependent RNA polymerase and L106L/F of ORF3a) that exhibit significant increasing trajectories (all p-values < 1.0*10-15). In the absence of relevant clinical data for these newly emerging mutations, it is important to monitor them closely. Two emerging mutations may be of particular concern: the N1192S mutation in spike protein locates in an extremely highly conserved region of all human coronaviruses that is integral to the viral fusion process, and the F694Y mutation in the RNA polymerase may induce conformational changes that could impact Remdesivir binding.
A Meta-Analysis of Accuracy and Sensitivity of CRISPR and Chest CT in COVID‑19 Diagnosis
Authors: gao chun , Jiang Jing-Jing , Feng Fu-Juan , Wang Cai-Hong , Wang Rong , Yao Xiao-Wen , Zhou Yu-Xia , Shao Hui-Juan , Ma Xue-Feng , Huang Jun , Zhang Jiu-Cong , Yu Xiao-Hui 
Published: February 2022

Abstract
Objective To clarify the accuracy of clusters of regularly spaced short palindrome repeats (CRISPR) technology and chest CT in the diagnosis of Corona Virus Disease2019(COVID-19). Methods The term "Corona Virus Disease " "clustered regularly spaced short palindromic repeats" "CRISPR", "chest CT", "sensitivity and specificity" as the subject words or keywords were searched in databases such as Pubmed, Embase, Cochrane Library, Wiley and Scopus and Chinese academic databases (such as CNKI, Wanfang and Chongqing VIP data) for relevant literature on the use of CRISPR technology and chest CT for the diagnosis of COVID-19. Meta-analysis was performed after literature screening, quality assessment and data extraction .Results A total of 418 articles were retrieved, and 17 articles were finally included. The results showed that the combined sensitivity of CRISPR technology for diagnosing new coronary pneumonia infection was 0.96 [95% CI (0.93, 0.98)], and the combined specificity was 1.00 [95% CI (0.92, 1.00)], the combined positive likelihood ratio is 458.69 [95%CI (11.51, 18280.8)], the combined negative likelihood ratio is 0.04 [95% CI (0.02, 0.07)], the area under the SROC curve is 0.99 [95%CI(0.97,0.99)]. The combined sensitivity of chest CT in diagnosing new coronary pneumonia infection was 0.94 [95%CI (0.83, 0.98)], combined specificity was 0.55 [95% CI (0.22, 0.83)], combined diagnostic odds ratio was 19.90 [95% CI (7.88, 50.25)], the combined positive likelihood ratio is 2.08 [95%CI (1.00, 4.32)], the combined negative likelihood ratio is 0.10 [95% CI (0.05, 0.23)], the area under the SROC curve is 0.91 [95% CI (0.88, 0.93)]. The Deek funnel chart indicates that there is no potential publication bias among the included studies (PCRISPR = 0.03, P chest CT = 0.55). Conclusion CRISPR technology has a better ability to detect infections in patients with COVID-19, and is better than chest CT in disease diagnosis. CRISPR technology, especially non-SHERLOCK type and multi-target gene detection, can be used to diagnose COVID-19 with higher accuracy ,and can be used for large-scale population screening.
Topical Antibiotics Reduce CD11c+ Cell Numbers in the Healthy Murine Cornea and Modulate their Response to Contact Lens Wear
Authors: Datta Ananya , Lee Justin , Truong Tiffany , Evans David J. , Fleiszig Suzanne M. J. 
Published: February 2022

Abstract
Previously we reported contact lens-induced CD11c + cell responses in healthy mouse corneas, a phenomenon that also occurs in humans. To test involvement of ocular-associated bacteria, we examined the impact of topical antibiotics on corneal CD11c + cell populations during 24 h of lens wear. Corneas were treated with gentamicin and ofloxacin (0.3%) or gentamicin alone, some also treated prior to lens wear (24 h). Contralateral PBS-treated eyes served as controls. CD11c-YFP mice allowed CD11c + cell visualization. Viable ocular- or lens-associated bacteria were labeled using FISH (16S rRNA-targeted probe) or click-chemistry (alkDala). Antibiotic treatment reduced baseline CD11c + cell numbers without lens wear and suppressed CD11c + cell responses to lens wear if corneas were both pretreated and treated during wear. Few bacteria colonized corneas or lenses under any circumstances. Conjunctival commensals were significantly reduced by antibiotics with or without lens wear, but minimally impacted by lens wear alone. Deliberate inoculation with conjunctival commensals triggered CD11c + cell responses irrespective of antibiotic pretreatment. These results suggest that while lens wear does not necessarily increase quantifiable numbers of conjunctival commensals, those neutralized by antibiotics play a role in lens-associated CD11c + cell responses and maintaining baseline CD11c + cell populations.