bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hereditary spastic paraplegia SPG13 mutation increases structural stability and ATPase activity of human mitochondrial chaperonin
Authors: Chen Lingling , Syed Aiza , Balaji Adhitya 
Published: October 2022

AbstractHuman mitochondrial chaperonin mHsp60 is broadly associated with various human health conditions and the V72I mutation in mHsp60 causes a form of hereditary spastic paraplegia, a neurodegenerative disease. The main function of mHsp60 is to assist folding of mitochondrial proteins in an ATP-dependent manner. In this study, we unexpectedly found that mutant mHsp60V72I was more stable structurally and more active in the ATPase activity than the wildtype. Analysis of our recently solved cryo-EM structure of mHsp60 revealed allosteric roles of V72I in structural stability and ATPase activity, which were supported by studies including those using the V72A mutation. Despite with the increases in structural stability and ATPase activity, mHsp60V72I was less efficient in folding malate dehydrogenase, a putative mHsp60 substrate protein in mitochondria and also commonly used in chaperonin studies. In addition, although mHsp60V72I along with its cochaperonin mHsp10 was able to substitute the E. coli chaperonin system in supporting cell growth under normal temperature of 37 °C, it was unable under heat shock temperature of 42 °C. Our results support the importance of structural dynamics and an optimal ATP turnover that mHsp60 has evolved for its function and physiology. We propose that unproductive energy utilization, or hyperactive ATPase activity and compromised folding function, not mutually exclusive, are responsible for the V72I pathology in neurodegenerative disease.
Demonstration of 100 Gbps coherent free-space optical communications at LEO tracking rates
Authors: Walsh Shane M. , Karpathakis Skevos F. E. , McCann Ayden S. , Dix-Matthews Benjamin P. , Frost Alex M. , Gozzard David R. , Gravestock Charles T. , Schediwy Sascha W. 
Published: October 2022

AbstractFree-space optical communications are poised to alleviate the data-flow bottleneck experienced by spacecraft as traditional radio frequencies reach their practical limit. While enabling orders-of-magnitude gains in data rates, optical signals impose much stricter pointing requirements and are strongly affected by atmospheric turbulence. Coherent detection methods, which capitalize fully on the available degrees of freedom to maximize data capacity, have the added complication of needing to couple the received signal into single-mode fiber. In this paper we present results from a coherent 1550 nm link across turbulent atmosphere between a deployable optical terminal and a drone-mounted retroreflector. Through 10 Hz machine vision optical tracking with nested 200 Hz tip/tilt adaptive optics stabilisation, we corrected for pointing errors and atmospheric turbulence to maintain robust single mode fiber coupling, resulting in an uninterrupted 100 Gbps optical data link while tracking at angular rates of up to 1.5 deg/s, equivalent to that of spacecraft in low earth orbit. With the greater data capacity of coherent communications and compatibility with extant fiber-based technologies being demonstrated across static links, ground-to-low earth orbit links of Terabits per second can ultimately be achieved with capable ground stations.
Efficacy and safety of dulaglutide compared with the first-line hypoglycemic drugs in Asian patients with type 2 diabetes: a systematic review and meta-analysis
Authors: Yu Bin , Lin Fei , Wang Maoru , Ning Hong , Ling Baodong , Rao Youyi 
Published: October 2022

AbstractTo assess the efficacy and safety of dulaglutide in the treatment of Asian type 2 diabetes mellitus (T2DM), along with first-line hypoglycemic drugs. Systematic review and meta-analysis. Cochrane Library, Pubmed, Embase, and www.clinicaltrials.gov databases were searched from inception to September 27, 2022. The studies evaluating adults (≥ 18 years) undergoing dulaglutide (0.75 mg and 1.5 mg) and first-line hypoglycemic drugs were considered. There were only English languages. We used Stata 12.0 software to detect the risk of bias. 4 randomized controlled trials (RCTs), and 1 observational study. Both dulaglutide 0.75 mg dose group and 1.5 mg dose group could significantly reduce HbA1c [Dulaglutide 0.75 mg: WMD = − 0.20, 95% CI (− 0.28, − 0.11), P < 0.0001; Dulaglutide 1.5 mg: WMD = − 0.49, 95% CI (− 0.67, − 0.30), P < 0.0001] in Asian T2DM patients. In reducing fasting blood glucose (FBG) level, there was no significant difference observed in 2 dose groups. The body weight of patients in both dulaglutide dose groups was significantly reduced. In safety, the incidence of adverse events in the dulaglutide 0.75 mg dose group was slightly higher than that in the first-line drug group, but there was no statistically significant difference in the incidence of adverse events between the 1.5 mg dose group and the first-line drug group. Furthermore, the incidences of hypoglycemic events in both groups were higher than that in the first-line drug group. Two doses of dulaglutide showed better efficacy for Asian T2DM patients, but patients should be vigilant about the occurrence of hypoglycemia and gastrointestinal discomfort. However, more number and better quality of RCTs are suggested to confirm long-term safety and efficacy.
Deoxygenation lowers the thermal threshold of coral bleaching
Authors: Alderdice Rachel , Perna Gabriela , Cárdenas Anny , Hume Benjamin C. C. , Wolf Martin , Kühl Michael , Pernice Mathieu , Suggett David J. , Voolstra Christian R. 
Published: October 2022

AbstractExposure to deoxygenation from climate warming and pollution is emerging as a contributing factor of coral bleaching and mortality. However, the combined effects of heating and deoxygenation on bleaching susceptibility remain unknown. Here, we employed short-term thermal stress assays to show that deoxygenated seawater can lower the thermal limit of an Acropora coral by as much as 1 °C or 0.4 °C based on bleaching index scores or dark-acclimated photosynthetic efficiencies, respectively. Using RNA-Seq, we show similar stress responses to heat with and without deoxygenated seawater, both activating putative key genes of the hypoxia-inducible factor response system indicative of cellular hypoxia. We also detect distinct deoxygenation responses, including a disruption of O2-dependent photo-reception/-protection, redox status, and activation of an immune response prior to the onset of bleaching. Thus, corals are even more vulnerable when faced with heat stress in deoxygenated waters. This highlights the need to integrate dissolved O2 measurements into global monitoring programs of coral reefs.
Innovative binary sorption of Cobalt(II) and methylene blue by Sargassum latifolium using Taguchi and hybrid artificial neural network paradigms
Authors: Moussa Zeiad , Ghoniem Abeer A. , Elsayed Ashraf , Alotaibi Amenah S. , Alenzi Asma Massad , Hamed Sahar E. , Elattar Khaled M. , Saber WesamEldin I. A. 
Published: October 2022

AbstractThe present investigation has been designed by Taguchi and hybrid artificial neural network (ANN) paradigms to improve and optimize the binary sorption of Cobalt(II) and methylene blue (MB) from an aqueous solution, depending on modifying physicochemical conditions to generate an appropriate constitution for a highly efficient biosorption by the alga; Sargassum latifolium. Concerning Taguchi's design, the predicted values of the two responses were comparable to actual ones. The biosorption of Cobalt(II) ions was more efficient than MB, the supreme biosorption of Cobalt(II) was verified in run L21 (93.28%), with the highest S/N ratio being 39.40. The highest biosorption of MB was reached in run L22 (74.04%), with a S/N ratio of 37.39. The R2 and adjusted R2 were in reasonable values, indicating the validity of the model. The hybrid ANN model has exclusively emerged herein to optimize the biosorption of both Cobalt(II) and MB simultaneously, therefore, the ANN model was better than the Taguchi design. The predicted values of Cobalt(II) and MB biosorption were more obedience to the ANN model. The SEM analysis of the surface of S. latifolium showed mosaic form with massive particles, as crosslinking of biomolecules of the algal surface in the presence of Cobalt(II) and MB. Viewing FTIR analysis showed active groups e.g., hydroxyl, α, β-unsaturated ester, α, β-unsaturated ketone, N–O, and aromatic amine. To the best of our knowledge, there are no reports deeming the binary sorption of Cobalt(II) and MB ions by S. latifolium during Taguchi orthogonal arrays and hybrid ANN.
Lithium deportment by size of a calcined spodumene ore
Authors: Nazir Muhammad Kashif , Dyer Laurence , Tadesse Bogale , Albijanic Boris , Kashif Nadia 
Published: October 2022

AbstractCalcination of spodumene is used to convert α-spodumene to more reactive β-spodumene, has been shown to greatly impact the physical characteristics of some of the components in the ore. This work investigates the energy efficiency of different grinding circuits used for upgrading the lithium content in the finer fraction of the calcined spodumene ore. The results showed that closed-circuit grinding resulted in 89% lithium recovery of the finest size fractions (− 0.6 mm) while open-circuit grinding led to 65% lithium recovery for the same grinding time. Closed-circuit grinding consumed lower energy than open-circuit grinding. The grade of the finest size fraction in the case of the open-circuit grinding was 1.7 times more than that in the case of the closed-circuit grinding. This work shows the potential of using different grinding modes to maximize energy efficiency and lithium deportment by size. However, it is suggested that open circuit grinding should be used for beneficiations of spodumene ores.
Opto-mechanically generated resonant field enhancement
Authors: Fresno-Hernández Alicia , Marqués Manuel I. 
Published: October 2022

AbstractA link between the resonant cumulative field enhancement experienced by a chain of plasmonic nanoparticles in a light field and the orientation of the chain with respect to the field is obtained. We calculate analytically the optical torque and the equilibrium configuration and we show how stable orientations are triggered by the geometric resonance conditions. Analytical predictions are checked using numerical calculations based on the coupled dipoles method (CDA) for the particular case of a chain of silver nanoparticles. The reported resonance driven optical torque allows for a tuning of the orientation of the chain depending on radiation’s wavelength.
Catalytic nanozyme Zn/Cl-doped carbon quantum dots as ratiometric fluorescent probe for sequential on-off-on detection of riboflavin, Cu2+ and thiamine
Authors: Dadkhah Sahar , Mehdinia Ali , Jabbari Ali , Manbohi Ahmad 
Published: October 2022

AbstractA novel metal-doped Zn/Cl carbon quantum dots (Zn/Cl-CQDs) was developed successfully as ratiometric fluorescent probes for the sequential on-off-on detection of riboflavin, Cu2+ ion and thiamine. The excellent catalytic performance of the Zn/Cl-CQDs nanozyme serves as an ideal platform for sensitive detection of thiamine. Due to the addition of riboflavin to the Zn/Cl-CQDs, the blue emission peak of Zn/Cl-CQDs at 440 nm remains unaffected and used as an internal reference approach, while the green emission peak of riboflavin at 520 nm appeared and increased remarkably. Following the presence of Cu2+, a quenching blue fluorescence signal of Zn/Cl-CQDs was observed which resulted in consequent fluorescent ‘turn-off’ response toward Cu2+ ion. Finally, upon the addition of thiamine to the above solution under alkaline condition, the blue emission of Zn/Cl-CQDs was gradually recovered. The prepared Zn/Cl-CQDs could act as a nanozyme catalyst for directly catalyzing the oxidation of non-fluorescent substrate of thiamine to produce highly fluorescent substrate of thiochrome. As a result, the blue fluorescence emission peak at 440 nm was recovered. Eventually, the sequential detection properties of ratiometric probes for riboflavin, Cu2+ ion and thiamine were successfully applied in VB2 tablets, drinking water and VB1 tablet with good recoveries of 96.21%, 98.25% and 98.44%, respectively.
A novel method to monitor COVID-19 fatality rate in real-time, a key metric to guide public health policy
Authors: Qu Yuanke , Lee Chun Yin , Lam K. F. 
Published: October 2022

AbstractAn accurate estimator of the real-time fatality rate is warranted to monitor the progress of ongoing epidemics, hence facilitating the policy-making process. However, most of the existing estimators fail to capture the time-varying nature of the fatality rate and are often biased in practice. A simple real-time fatality rate estimator with adjustment for reporting delays is proposed in this paper using the fused lasso technique. This approach is easy to use and can be broadly applied to public health practice as only basic epidemiological data are required. A large-scale simulation study suggests that the proposed estimator is a reliable benchmark for formulating public health policies during an epidemic with high accuracy and sensitivity in capturing the changes in the fatality rate over time, while the other two commonly-used case fatality rate estimators may convey delayed or even misleading signals of the true situation. The application to the COVID-19 data in Germany between January 2020 and January 2022 demonstrates the importance of the social restrictions in the early phase of the pandemic when vaccines were not available, and the beneficial effects of vaccination in suppressing the fatality rate to a low level since August 2021 irrespective of the rebound in infections driven by the more infectious Delta and Omicron variants during the fourth wave.
Grassland coverage change and its humanity effect factors quantitative assessment in Zhejiang province, China, 1980–2018
Authors: Lü Linyou , Zhao Yan , Chu Lei , Wang Yongcui , Zhou Quanlai 
Published: October 2022

AbstractThis study aims to make clear of grassland coverage change and quantitative assessment its effect factors. We collected the data from the National Bureau of Statistics (http://www.stats.gov.cn) and "China 20th Century Land Use/Cover Change (LUCC) Spatio-temporal Platform". Grassland coverage area showed an upward trend from 1980 to 1990, and the grassland coverage area is gradually decreasing from 1990 to 2000, and the grassland coverage area has not changed much from 2000 to 2018. The medium-coverage grassland area has the highest correlation with the total population, and the high-coverage grassland area has the lowest correlation with the total population. Land use types and the composite of gross agricultural output have influence on grassland coverage area. It is hoped that relevant policies should consider land use types and ecological benefits while balancing economic development and urban development.
On the future zonal contrasts of equatorial Pacific climate: Perspectives from Observations, Simulations, and Theories
Authors: Lee Sukyoung , L’Heureux Michelle , Wittenberg Andrew T. , Seager Richard , O’Gorman Paul A. , Johnson Nathaniel C. 
Published: October 2022

AbstractChanges in the zonal gradients of sea surface temperature (SST) across the equatorial Pacific have major consequences for global climate. Therefore, accurate future projections of these tropical Pacific gradients are of paramount importance for climate mitigation and adaptation. Yet there is evidence of a dichotomy between observed historical gradient trends and those simulated by climate models. Observational records appear to show a “La Niña-like” strengthening of the zonal SST gradient over the past century, whereas most climate model simulations project “El Niño-like” changes toward a weaker gradient. Here, studies of these equatorial Pacific climate trends are reviewed, focusing first on data analyses and climate model simulations, then on theories that favor either enhanced or weakened zonal SST gradients, and then on notable consequences of the SST gradient trends. We conclude that the present divergence between the historical model simulations and the observed trends likely either reflects an error in the model’s forced response, or an underestimate of the multi-decadal internal variability by the models. A better understanding of the fundamental mechanisms of both forced response and natural variability is needed to reduce the uncertainty. Finally, we offer recommendations for future research directions and decision-making for climate risk mitigation.
Rewiring glucose metabolism improves 5-FU efficacy in p53-deficient/KRASG12D glycolytic colorectal tumors
Authors: Ludikhuize Marlies C. , Gevers Sira , Nguyen Nguyen T. B. , Meerlo Maaike , Roudbari S. Khadijeh Shafiei , Gulersonmez M. Can , Stigter Edwin C. A. , Drost Jarno , Clevers Hans , Burgering Boudewijn M. T. , Rodríguez Colman Maria J. 
Published: October 2022

AbstractDespite the fact that 5-fluorouracil (5-FU) is the backbone for chemotherapy in colorectal cancer (CRC), the response rates in patients is limited to 50%. The mechanisms underlying 5-FU toxicity are debated, limiting the development of strategies to improve its efficacy. How fundamental aspects of cancer, such as driver mutations and phenotypic heterogeneity, relate to the 5-FU response remains obscure. This largely relies on the limited number of studies performed in pre-clinical models able to recapitulate the key features of CRC. Here, we analyzed the 5-FU response in patient-derived organoids that reproduce the different stages of CRC. We find that 5-FU induces pyrimidine imbalance, which leads to DNA damage and cell death in the actively proliferating cancer cells deficient in p53. Importantly, p53-deficiency leads to cell death due to impaired cell cycle arrest. Moreover, we find that targeting the Warburg effect in KRASG12D glycolytic tumor organoids enhances 5-FU toxicity by further altering the nucleotide pool and, importantly, without affecting non-transformed WT cells. Thus, p53 emerges as an important factor in determining the 5-FU response, and targeting cancer metabolism in combination with replication stress-inducing chemotherapies emerges as a promising strategy for CRC treatment.
Metformin alleviates HFD-induced oxidative stress in hepatocyte via activating SIRT6/PGC-1α/ENDOG signaling
Authors: Gao Shuya , Yang Qingchen , Liu Zekun , Kong Weixian , Chen Jiawen , Li Xie , Peng Yue , Bao Mengmeng , Bian Xiaohong , Zhang Yuexin , Jiang Qizhou , Li Zhe , Zhang Yubin , Yan Fangrong , Ye Junmei 
Published: October 2022

Abstract
Metformin is accepted as a first-line drug for the therapy of Type 2 diabetes (T2D), while its mechanism is still controversial. In the present study, by taking advantage of mouse model of high-fat-diet (HFD)-induced obesity and primary mouse hepatocytes (PMHCs) as well as human hepatocyte L02 cell line, we aimed to investigate the involvement of SIRTs during the application of metformin for the therapy of T2D. Our data evidenced that during HFD-induced obesity, there was elevation of nucleus protein acetylation. Analysis of liver tissue showed that among all SIRT members, SIRT6 expression was significantly down-regulated during HFD feeding, which was sustained to regular level with metformin administration. Our result also showed that SIRT6 suppressed intracellular oxidative stress upon FAs stimulation in PMHCs and L02 cells. Mechanistically, SIRT6, but not SIRT1 promoted PGC-1α expression. We further prove that ENDOG is downstream of PGC-1α. In addition, we evidenced that ENDOG protects hepatocytes from lipid-induced oxidative stress, and down-regulation of Endog blunted the protective role of metformin in defending against FAs-induced oxidative stress. Our study established a novel mechanism of metformin in counteracting lipid-induced hepatic injury via activating SIRT6/PGC-1α/ENDOG signaling, thus providing novel targets of metformin in the therapy of T2D.
Predicting network flows from speeds using open data and transfer learning
Authors: Mahajan Vishal , Cantelmo Guido , Rothfeld Raoul , Antoniou Constantinos 
Published: October 2022

AbstractTraffic flow/volume data are commonly used to calibrate and validate traffic simulation models. However, these data are generally obtained from stationary sensors (e.g. loop detectors), which are expensive to install and maintain and cover a small number of locations in the transport network. On the other hand, Floating Car Data (FCD) are readily available at the network level, usually from a sample of vehicles. We present an indirect traffic flow estimation approach using transfer learning to address the traffic flow data scarcity and model generalization across cities. Using two cities (Paris and Madrid) as study areas, we demonstrate the indirect estimation using only exogenous features for flow prediction, mirroring limited predictive features without past link flows. Subsequently, we use the model pre‐trained on data from Paris city and test on data from Madrid city, and investigate the scenarios for successful transfer learning. Overall, the training set must adequately capture the flow‐speed relationship for successful indirect flow estimation. Transfer learning is beneficial when the data for the target task is minimal, in which case transferred models outperform newly trained models from scratch. Using real‐world and publicly available data, our approach and models can help scale a smaller traffic flow dataset to a larger sample across cities.
The effect of shock wave properties on the release timings of solar energetic particles
Authors: Kouloumvakos A. , Vainio R. , Gieseler J. , Price D. J. 
Published: October 2022

Context. Fast and wide coronal mass ejections (CMEs) and CME-driven shock waves are capable of accelerating solar energetic particles (SEPs) and releasing them in very distant locations in the solar corona and near-Sun interplanetary space. SEP events have a variety of characteristics in their release times and particle anisotropies. In some events, specifics of the SEP release times are thought to be difficult to reconcile with the scenario that a propagating shock wave is responsible for the SEP release.
Aims. Despite the apparent difficulties posed by the shock scenario, many studies have not considered the properties of the propagating shock waves when making a connection with SEP release. This could probably resolve some of the issues and would help us to delve into and understand more important issues such as the effect of the shock acceleration efficiency on the observed characteristics of the SEP timings and the role of particle transport. This study aims to approach these issues from the shock wave perspective and elucidate some of these aspects.
Methods. We constructed a simple 2D geometrical model to describe the propagation and longitudinal extension of a disturbance. We used this model to examine the longitudinal extension of the wave front from the eruption site as a function of time, to calculate the connection times as a function of the longitudinal separation angle, and to determine the shock parameters at any connection point. We examined how the kinematic and geometric properties of the disturbance could affect the timings of the SEP releases at different heliolongitudes.
Results. We show that the extension of a wave close to the solar surface may not always indicate when a magnetic connection is established for the first time. The first connection times depend on both the kinematics and geometry of the propagating wave. A shock-related SEP release process can produce a large event-to-event variation in the relationship between the connection and release times and the separation angle to the eruption site. The evolution of the shock geometry and shock strength at the field lines connected to an observer are important parameters for the observed characteristic of the release times.