bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reference‐dependent age weighting of quality‐adjusted life years
Authors: Attema Arthur E. , Brouwer Werner B. F. , Pinto‐Prades Jose Luis 
Published: September 2022

ABSTRACTPeople do not only care about maximizing health gains but also about their distribution. For example, they give more weight to younger patients than older patients. This pilot study aims to investigate if age weighting is reinforced by loss aversion if young people are falling behind one's perceived ‘normal’ quality of life (QoL), while older people do not. We apply a person trade‐off method in a large representative sample (n = 990) to estimate age weighting factors. We also measure QoL levels that individuals regard as ‘normal’ for different ages, serving as reference points. We observe a considerable amount of age weighting, with 20‐year‐old patients on average receiving 1.7 times as much weight as 80‐year‐old patients. Perceived ‘normal’ QoL rapidly decreases with age of a patient. Older people are more optimistic about what constitutes ‘normal QoL’ than younger people, but they express a faster decline in normal QoL due to aging. Respondents who view all improvements to be gain enlarging show the least age weighting, but loss aversion cannot explain the results. Still, one's age‐related reference level is an important predictor of age weights. Given the explorative nature of this study, further studies are called for to generate more robust evidence.
Well‐being right before and after a permanent nursing home admission
Authors: Bom Judith , Bakx Pieter , Rellstab Sara 
Published: September 2022

AbstractPermanent nursing home (NH) admissions are a frequent and major life event aimed at maintaining quality of life in old age. Yet, insights into the impact of a NH admission on well‐being are scarce and inconclusive. We evaluate the effect of a NH admission on domains of well‐being among those who are admitted using event study methodology for cross‐sections combined with inverse probability weighting. We apply this doubly robust approach to Dutch survey data on well‐being linked to extensive administrative data on NH admissions, health, and socio‐economic status. We find that a NH admission leads to a temporary increase in loneliness, the risk of anxiety and depression, and a loss of control over one's life. However, these scores revert to pre‐admission levels after 6 months. These findings may contribute to better‐informed individual‐level and policy decisions about potential NH entry and aging in place policies.
Family CEO successor and firm performance: The moderating role of sustainable HRM practices and corporate philanthropy
Authors: Su Yiyi , Xia Jun , Zahra Shaker A. , Ding Jiayan 
Published: September 2022

AbstractWe develop and test a multi‐stakeholder perspective of intrafamily CEO succession by exploring how family CEO successors affect post‐succession firm performance under conditions of sustainable human resource management (sustainable HRM) practices toward employees and top managers, as well as corporate philanthropic activities in the broader community. Using a sample of 414 CEO successions in family firms listed on China's stock exchanges during 2008–2016, we find an insurance‐like effect of both sustainable HRM and corporate philanthropy in enhancing firm performance of family CEO successors. Our results also show that firms with family CEO successors will outperform those with nonfamily counterparts under conditions of high employee compensation, low top management team (TMT) compensation, and long TMT tenure. Our findings suggest that sustainable HRM and corporate philanthropy complement rather than substitute in strengthening family leadership during CEO succession.
Comparison between enzyme‐linked immunospot assay and intracellular cytokine flow cytometry assays for the evaluation of T cell response to SARS‐CoV‐2 after symptomatic COVID‐19
Authors: Villemonteix Juliette , Cohen Laure , Guihot Amélie , Guérin Valérie , Moulin Clémentine , Caseris Marion , Carol Agnès , Bonacorsi Stéphane , Carcelain Guislaine 
Published: September 2022

AbstractIntroductionEvaluation of different cell‐based assays for the study of adaptive immune responses against SARS‐CoV‐2 is crucial for studying long‐term and vaccine‐induced immunity.MethodsEnzyme‐linked immunospot assay (ELISpot) and intracellular cytokine staining (ICS) using peptide pools spanning the spike protein and nucleoprotein of SARS‐CoV‐2 were performed in 25 patients who recovered from paucisymptomatic (n = 19) or severe COVID‐19 (n = 6).ResultsThe proportion of paucisymptomatic patients with detectable SARS‐CoV‐2 T cells was low, as only 44% exhibit a positive T cell response with the ICS and 67% with the ELISpot. The magnitude of SARS‐CoV‐2 T cell responses was low, both with ICS (median at 0.12% among total T cells) and ELISpot (median at 61 SFCs/million peripheral blood mononuclear cells [PBMC]) assays. Moreover, T cell responses in paucisymptomatic patients seemed lower than among patients with severe disease. In the paucisymptomatic patients, the two assays were well correlated with 76% of concordant responses and a Cohen's kappa of 55. Furthermore, in four patients SARS‐CoV‐2 T cells were detected by ELISpot but not with ICS. Short‐term culture could improve the detection of specific T cells.ConclusionsIn patients who recovered from paucisymptomatic COVID‐19, the proportion of detectable anti‐SARS‐CoV‐2 responses and their magnitude seemed lower than in patients with more severe symptoms. The ELISpot appeared to be more sensitive than the ICS assay. Short‐term culture revealed that paucisymptomatic patients had nonetheless few SARS‐CoV‐2 T cells at a very low rate in peripheral blood. These data indicate that various ex‐vivo assays may lead to different conclusions about the presence or absence of SARS‐CoV‐2 T cell immunity.
BMS‐202, a PD‐1/PD‐L1 inhibitor, decelerates the pro‑fibrotic effects of fibroblasts derived from scar tissues via ERK and TGFβ1/Smad signaling pathways
Authors: Cai Yuanyuan , Xiao Min , Li Xinqing , Zhou Shanyu , Sun Yangyang , Yu Wenyuan , Zhao Tianlan 
Published: September 2022

AbstractIntroductionHypertrophic scar (HS), a fibroproliferative disorder of the skin with some tumor‐like properties, is closely related to dysregulated inflammation. PD‐1/PD‐L1 inhibitor is a promising medication for cancer therapy as its potent functions on adaptive immune response; whether it could be a candidate for HS therapy has aroused our interest. This study aimed to explore the effect and the mechanism of BMS‐202, a PD‐1/PD‐L1 inhibitor, in HS.MethodsTen HS and adjacent normal skin tissues collected from HS patients were used to detect α‐SMA, collagen I, and PD‐L1 expression by Quantitative reverse transcription‐polymerase chain reaction and western blot (WB) analysis. Fibroblasts derived from HS tissues (HFBs) were exposed to diverse concentrations of BMS‐202, of which proliferation, migration, apoptosis, and collagen synthesis were evaluated by Cell Counting Kit‐8, wound healing, terminal deoxynucleotidyl transferase (TdT) dUTP Nick‐End labeling, and [3H]‑proline incorporation assays, respectively. The effect of BMS‐202 on α‐SMA and collagen I expression, and transforming growth factor beta 1 (TGFβ1)/Smad signaling in HFBs was also determined by WB and enzyme‐linked immunosorbent assay.ResultsThe expression level of PD‐L1 was significantly elevated in both HS tissues and HFBs, which was positively correlated with α‐SMA and collagen I expressions. BMS‐202 exerted a significant suppression effect on the cell proliferation, migration, collagen synthesis, and α‐SMA and collagen I expression of HFBs in a concentration‐dependent way; but did not affect apoptosis. Finally, BMS‐202 could reduce the phosphorylation of ERK1/2, Smad2, and Smad3, and the TGFβ1 expression once its concentration reached 2.5 nM.ConclusionBMS‐202 effectively suppressed proliferation, migration, and extracellular matrix deposition of HFBs, potentially through the regulation of the ERK and TGFβ1/Smad signaling pathways.
Microscopic Raman illustrating antitumor enhancement effects by the combination drugs of γ‐secretase inhibitor and cisplatin on osteosarcoma cells
Authors: Li Jie , Li Jing , Wang Haifeng , Chen Yishen , Qin Jie , Zeng Haishan , Wang Kaige , Wang Shuang 
Published: September 2022

AbstractBy using Raman microspectroscopy, it aims to elucidate the cellular variations caused by the combination drug of γ‐secretase inhibitor (DAPT) and cisplatin in osteosarcoma (OS) cells. Illustrated by the obtained results of spectral analysis, the intracellular composition significantly changed after combined drug actions compared to the solo DAPT treatment, indicating the synergistic effect of DAPT combined with cisplatin on OS cells. Meanwhile, multivariate curve resolution‐alternating least squares (MCR‐ALS) algorithm was utilized to address the biochemical constitution changes in all investigated groups including the untreated (UT), DAPT (40D) and combined drug (40D + 20C) treated cells. K‐means cluster and univariate imaging were both utilized to visualize the changes in subcellular morphology and biochemical distribution. The presented study provides a unique understanding on the cellular responses to DAPT combined with cisplatin from the natural biochemical perspectives, and laids an experimental foundation for exploring the therapeutic strategies of other combined anticancer drugs in cancer cell model.
Impact of negative interest rate policy on the swap market in Japan: Comparative analysis before and after yield curve control
Authors: Ito Takayasu 
Published: September 2022

AbstractNo co‐movement is found among swap rates with a maturity of 10, 15, 20, and 30 years before the introduction of yield curve control (YCC) under Negative Interest Rate Policy (NIRP). On the other hand, they co‐move after the introduction, driven by a single common trend. No single pair of swap rates move together before the introduction, but every pair move together after the introduction. The function of the swap market was lost after the introduction of NIRP. This caused uncertainties regarding the formation of the yield curve among the market participants in the super long zone of the swap market. After the Bank of Japan (BOJ) introduced YCC to move the yield curve upward, particularly in moving the 10‐year Japanese Government Bond (JGB) yield to around zero percent, structural changes took place not only in JGB but also in swap markets.
Evaluation of Hemoglobin Response to Treatment With Enarodustat Using Pharmacometric Approach in Japanese Anemic Patients With Chronic Kidney Disease
Authors: Naruhashi Shinya , Fujii Takashi , Yamada Hiroyuki , Pai Sudhakar M. , Ninomiya Noriko 
Published: September 2022

AbstractEnarodustat (JTZ‐951) is a hypoxia‐inducible factor prolyl hydroxylase inhibitor that has been approved and marketed in Japan for patients with anemia with chronic kidney disease (CKD). The pharmacometric approach was applied to assess the relationship between plasma concentrations of enarodustat and hemoglobin (Hb) levels, and to provide information regarding the optimal use of enarodustat in clinical practice by simulations based on the pharmacokinetic and pharmacodynamic (PK/PD) model that was developed. The PK/PD data of enarodusat obtained from phase 2 and phase 3 studies in Japanese patients with CKD were well described by the models: a 1‐compartment model with first‐order absorption and elimination for PK, and a semimechanistic model based on transit compartment model for PD. Although several factors were identified as statistically significant covariates on the PK/PD of enarodustat, model‐based simulations showed that none of them had clinically relevant impacts on the treatment effect (ie, Hb levels) of enarodustat. Hence, enarodustat treatment provides the stable Hb control with the initial dose (hemodialysis‐dependent CKD: 4 mg/day, non–dialysis‐dependent CKD: 2 mg/day) and maintenance dose (1–8 mg/day) to the patients with varied demographic characteristics.
Ammonium load determines the performance of a continuous nitritation process and the related microbial communities even under excesses of O2 supply
Authors: Aguilar‐Pichardo Monserrat , Valenzuela Edgardo I. , Quijano Guillermo 
Published: September 2022

AbstractBackgroundPartial oxidation of ammonium to nitrite, process known as nitritation, has important advantages over complete nitrification, including energy savings due to a reduced aeration, a lower organic carbon requirement for denitrification, a reduced production of sludge, and the potential use of nitrite in anammox and anoxic biogas purification technologies. Dissolved O2 (DO) concentration commonly has been regarded as the most important parameter to be controlled for achieving a successful nitritation process.ResultsThe present study showed that setting average DO values below 1 g O2 m−3 was insufficient to achieve a high and stable nitritation at ammonium loading rates between 2.5 to 5.1 g N‐NH4+ m−3 h−1. Under these conditions, the predominance of nitrite oxidizing bacteria (NOB) resulted in ammonium oxidation selectivity to nitrite from 5.2 ± 3.6% to 42.8 ± 21%. Increasing the ammonium loading to 10.1 gN‐NH4+ m−3 h−1 together with an average DO value of 0.9 ± 0.6 gO2 m−3 resulted in an ammonium oxidation efficiency of 94 ± 1.1% and ammonium oxidation selectivity to nitrite >90%.ConclusionContinuous nitritation was achieved in a single reactor provided with an easy‐to‐implement aeration strategy. The ammonium loading rate was the most important parameter to be controlled, which inhibited NOB above a threshold value. NOB inhibition was mediated mainly by free nitrous acid, and by free ammonia to a lesser extent. Increasing the ammonium loading rate led to an enrichment of ammonium oxidizing bacteria of the Nitrosomonas genus. © 2022 Society of Chemical Industry (SCI).
Preparation of porous macromolecule oxazine poly‐ionic liquid solid acid catalyst and its application in an esterification reaction
Authors: Wang Yajuan , Liu Wenqiang , Li Jiangxin , Zhang Juan 
Published: September 2022

AbstractBackgroundChinese research on citrate is typically into plasticizer alternatives to the carcinogenic phthalate (PAEs) plasticizers. PAEs plasticizers can induce obesity and metabolic disorders, whereas citric acid vinegar plasticizers have the advantages of low biological toxicity and environmental friendliness.ResultsNovel porous macromolecule oxazine poly‐ionic liquid solid acid catalysts were prepared and characterized by scanning electron microscopy (SEM), Brunner−Emmet−Teller(BET) measurements, Fourier transform infrared spectroscopy (FTIR), energy dispersive spectrometer (EDS) and thermogravimetric analysis (TGA). Characterization results showed that the synthesized catalyst was a porous polymer with good heat stability. The catalytic performance of the catalyst was evaluated by synthesis of tributyl citrate from citric acid and n‐butanol.ConclusionThe optimal reaction conditions were determined as 9% (wt%) citrate mass. The experimental results showed that the optimal reaction conditions were determined as amount of catalyst 9% (wt% citrate mass), 150 °C reaction temperature, 3.5 h reaction time and 1:3 ratio of citric acid and n‐butanol. Under optimal conditions, the conversion rate of citrate acid was ≤91.74%. The catalytic activity did not reduce obviously after being reused for ten times. Then, the reaction conditions were fited by the surface optimization method. The fitted optimum conditions were as follows: 8.881% amount of catalyst (mass of citric acid), 148.259 °C reaction temperature, 3.331 h reaction time, 1:2.958 citric acid:n‐butanol ratio; this gave a conversion rate of citric acid of ≤92.998%. The simulation results are in good agreement with the experimental results. © 2022 Society of Chemical Industry (SCI).
Separation of microalgae cells in a microfluidic chip based on AC Dielectrophoresis
Authors: Wang Yanjuan , Zhao Kai , Tong Ning , Wang Junsheng 
Published: September 2022

AbstractBACKGROUNDMicroalgae is an important natural resource with extensive applications in various fields, such as health products, animal feed, biopharmaceuticals, and clean energy. However, before the research and utilization of microalgae resources, the microalgae cells of interest must be separated from hybrid strains and bacteria. The traditional method of microalgae separation requires manual labor under the microscope that is time‐consuming and labor‐intensive.RESULTSHerein, a novel microalgae cell separation method based on the dielectrophoresis (DEP) technique was proposed. A unique DEP separation chip with a 3D electrode was designed. Chlorella and Closterium were used as the experimental samples to verify the effectiveness of the proposed method because they have similar volumes and very different electrical properties. Within a certain frequency range, Chlorella suffers from negative dielectrophoresis (nDEP) force and Closterium suffers from positive dielectrophoresis (pDEP) force, so their separation can be achieved. The separation efficiency exceeded 90%. In addition, the DEP response of the Chlorella and Closterium were studied, and the DEP spectra of the two microalgae cells were obtained. Moreover, the effects of DEP force on cell viability under different voltages and frequencies were experimentally analyzed.CONCLUSIONThese findings will contribute to DEP technology, to the portable and harmless separation of microalgae, and to research on the dielectric properties of microalgae cells. © 2022 Society of Chemical Industry (SCI).
An investigation into the electro‐tolerance of Escherichia coli treated by radio frequency electric fields
Authors: Rezaeimotlagh Adel , Serasinghe Pulasthi , Manefield Michael , Trujillo Francisco J. 
Published: September 2022

AbstractBACKGROUNDRadio frequency electric field (RFEF) is a novel technology for the microbial disinfection of liquids such as water and fruit juices. Here we investigated the development of microbial tolerance against RFEF to assess the long‐term suitability of this technology. We applied a constant electric field, outlet temperature and treatment time of 14 kV cm−1, 40 °C and 1.275 ms, respectively, to Escherichia coli inoculated in saline water to observe whether the descendants of the surviving bacteria could develop tolerance against RFEF. Escherichia coli that survived the RFEF treatment were recultivated and treated under similar processing conditions for 15 generations.RESULTSThe results showed that although inactivation levels due to RFEF treatments fluctuated during consecutive generations, no significant difference between inactivation at cycles 1 and 15 was observed. Furthermore, inactivation levels during consecutive generations hovered around an average value of 2.18 CFU mL−1. For short‐term effect, which compared untreated cells to treated ones at cycle 1, proteomics showed mixed trends of up‐ and down‐regulated differentially expressed proteins (DEPs). However, in the long term, which compared treated cells at cycles 1 and 15, DEPs involved in stress response, cell wall structure and antioxidant activities were all up‐regulated.CONCLUSIONThe results indicate that although cell wall is the main target for the effect of electric field, they suggest that, in the long term, oxidative stress may also be induced. © 2022 Society of Chemical Industry (SCI).
Self‐supported single‐wall carbon nanotube buckypaper membranes applied to air and water filtration
Authors: Berned‐Samatán Víctor , Jiménez Santiago , Rubio César , Téllez Carlos , Coronas Joaquín 
Published: September 2022

AbstractBackgroundThis work addresses the use of self‐supported single‐wall carbon nanotube (SWCNT) buckypapers as filters or membranes to treat air or water streams to reduce the concentration of pollutants.ResultsThe fabrication of the buckypapers was carried out by a facile filtration method. The performance of the buckypaper as an air filter was tested with a NaCl aerosol obtaining a permeance of 241 m3 m−2 h−1 bar−1 and a filtration efficiency of 99.9991%. The performance of the buckypaper as a water ultrafiltration (UF) membrane was studied separating ZIF‐8 nanoparticles of 30, 50 and 120 nm in diameter, achieving a permeance of 124 L·m−2 h−1 bar−1 and a rejection of 99.99%.ConclusionsA simple technique is described for the rapid fabrication of self‐supported SWCNT buckypaper membranes with a filtration method resulting in an excellent performance in both air and water filtration. In addition, because of the acquired control of ZIF‐8 crystallization, particles of different sizes of this material were synthesized ad hoc to serve as a reference solute of UF separation. © 2022 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
Preparation of Ce/ferroferric oxide/food waste‐derived biochar for aqueous Cr(VI) adsorption
Authors: Tie Jingxi , Zhang Meng , Shen Chenliang , Liu Haiyuan , Du Chunbao 
Published: September 2022

AbstractBACKGROUNDCr(VI) in aqueous solution is a serious concern that can harm human health by accumulating in the food chain. Meanwhile, the most common form of food waste (FW) disposal by landfill takes up a lot of area and pollutes the environment. As a consequence, FW was employed as a raw material in the production of food waste‐derived biochar (FWB), which was then modified to produce a Ce/Fe3O4/FWB composite for high‐efficiency Cr(VI) removalRESULTSThe structure, morphology, and properties of the Ce/Fe3O4/FWB composite were thoroughly investigated, as well as its Cr(VI) adsorption ability. The composite exhibited the highest Cr(VI) adsorption of 20.4 mg/g at pH 2. Furthermore, the adsorption fitted the pseudo‐second‐order kinetic model and the Langmuir model, with intra‐particle diffusion acting as the rate‐controlling step. The thermodynamic investigation demonstrated that Cr (VI) adsorption by Ce/Fe3O4/FWB was a spontaneous endothermic process. With increasing concentrations of Cl−, SO42−and PO43−, the Cr(VI) adsorption was weakened in the sequence of Cl−, SO42−and PO43−.CONCLUSIONAdsorbent preparation utilizing FW as raw material is a sustainable and environmentally beneficial method for FW reclaimation and Cr(VI)‐bearing wastewater treatment. © 2022 Society of Chemical Industry (SCI).
Concurrent light chain amyloidosis and proximal tubulopathy: Insights into different aggregation behavior—A case report
Authors: Feurstein Simone , Zoller Julian , Schwab Constantin , Schreiner Sarah , Mundt Heiko , Breitkreutz Iris , Schneider Brigitte , Beimler Jörg , Zeier Martin , Waldherr Rüdiger , Gröschel Stefan , Müller‐Tidow Carsten , Schönland Stefan O. , Hegenbart Ute 
Published: September 2022

AbstractDue to differences in the protein folding mechanisms, it is exceedingly rare for amyloid light chain (AL) amyloidosis and monoclonal gammopathy of renal significance (MGRS) to coexist. We herein report the first case of concurrent AL amyloidosis and a subclass of MGRS, light chain proximal tubulopathy (LCPT). The 53‐year‐old female was diagnosed with smoldering myeloma immunoglobulin G kappa and AL amyloidosis with deposits in fat and gastrointestinal tissue. The kidney biopsy did not show amyloid deposits but electron microscopy revealed the presence of LCPT with crystal formation in proximal tubular epithelial cells. This case illustrates the complex pathophysiology of protein deposition in monoclonal gammopathies.