bluuebunny/crossref_metadata_2025_split huggingface dataset:
SnO2/rGO nanocomposite for the detection of biomarkers of lung cancer
Authors: Shanmugasundaram Arunkumar , Lee Dong-Weon 
Published: September 2022

AbstractMetal oxide-based sensors have been widely used to detection biomarkers in exhaled breath for identification of various diseases such as asthma, diabetes, halitosis, and lung cancer. Herein, we proposed one step hydrothermal method for the preparation of SnO2 nanospheres and reduced graphene oxide incorporated SnO2 nanospheres for the detection of two important biomarkers such as decane and heptane from the exhaled breath of lung cancer patients. The as prepared materials are investigated in detail through various analytical techniques and the findings are consistent with each other. The sensing response of the proposed sensors were systematically investigated to enhance their sensing performance as a function of operating temperatures and gas concentration, and different analyte gases. The sensors showed maximum sensing response toward heptane and decane compared to other interfering gases such as hydrogen, carbon monoxide, acetone, ethanol, and methanol at 125 °C. The proposed sensors exhibit excellent detection range as low as 1 ppm with appreciably fast response and recovery time. Lung cancer patients may be easily screened using the proposed sensor, by detecting decane and heptane in their exhaled breath.
Toward a New Paradigm in Resistance Training by Means of Velocity Monitoring: A Critical and Challenging Narrative
Authors: González-Badillo Juan José , Sánchez-Medina Luis , Ribas-Serna Juan , Rodríguez-Rosell David 
Published: September 2022

AbstractFor more than a century, many concepts and several theories and principles pertaining to the goals, organization, methodology and evaluation of the effects of resistance training (RT) have been developed and discussed between coaches and scientists. This cumulative body of knowledge and practices has contributed substantially to the evolution of RT methodology. However, a detailed and rigorous examination of the existing literature reveals many inconsistencies that, unless resolved, could seriously hinder further progress in our field. The purpose of this review is to constructively expose, analyze and discuss a set of anomalies present in the current RT methodology, including: (a) the often inappropriate and misleading terminology used, (b) the need to clarify the aims of RT, (c) the very concept of maximal strength, (d) the control and monitoring of the resistance exercise dose, (e) the existing programming models and (f) the evaluation of training effects. A thorough and unbiased examination of these deficiencies could well lead to the adoption of a revised paradigm for RT. This new paradigm must guarantee a precise knowledge of the loads being applied, the effort they involve and their effects. To the best of our knowledge, currently this can only be achieved by monitoring repetition velocity during training. The main contribution of a velocity-based RT approach is that it provides the necessary information to know the actual training loads that induce a specific effect in each athlete. The correct adoption of this revised paradigm will provide coaches and strength and conditioning professionals with accurate and objective information concerning the applied load (relative load, level of effort and training effect). This knowledge is essential to make rational and informed decisions and to improve the training methodology itself.
Is Cumulative Load Associated with Injuries in Youth Team Sport? A Systematic Review
Authors: Sniffen Katie , Noel-London Kemba , Schaeffer Melody , Owoeye Oluwatoyosi 
Published: September 2022

AbstractBackgroundHigh cumulative external and internal load may predispose athletes to increased risk for injury across a variety of sports, competition levels, and age groups. However, evidence of an association between cumulative load and injury in youth sport remains inconclusive. The objective of this study was to determine the current evidence for cumulative load and injury risk relationships in youth team sport through a systematic review of the existing literature.MethodsA systematic review of the literature was performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Literature searches were conducted in PubMed, Web of Science, SCOPUS, and CINAHL for relevant articles published between January 2010 and April 2021. The authors conducted independent review and quality assessment of the eligible studies. Eleven articles evaluating youth (less than 18 years old) team sport were included for qualitative synthesis.ResultsFifty-nine percent (n = 39/66) of the relationships assessed revealed an association between cumulative load and injury across the team sports studied, including the presence of load–injury associations in 84% (n = 16/19) of assessments in youth soccer. Of those relationships where an association was present, 79% (n = 31/39) were positive associations between cumulative load and injury. Risk of bias assessment scores ranged from three to six out of seven possible (median = 5) for cohort studies and from four to seven out of 10 possible (median = 5.5) for cross-sectional studies.ConclusionsThere is some evidence for a positive association between load and injury in youth team sport. Youth soccer was the most studied team sport, and a substantial number of positive load–injury associations were reported. Current evidence lacks consistency in the measures and metrics used in defining load–injury relationships.Trial RegistrationPRISMA ID - CRD42020203622.
Developing and evaluating a SAFER model to screen for diabetes complications among people experiencing homelessness: a pilot study protocol
Authors: Scott Sara , Grewal Eshleen K. , Tariq Hamna , Campbell David J. T. 
Published: September 2022

Abstract
Background
Diabetes management combined with housing instability intersects, forcing individuals to triage competing needs and critical stressors, such as safety and shelter, with fundamental diabetes self-management tasks like attending healthcare appointments to screen for the complications of diabetes, leaving individuals overwhelmed and overburdened. We aim to address this disjuncture found within our current healthcare delivery system by providing point-of-care screening opportunities in a more patient-centered approach.

Method
We describe a pilot study of a novel clinical intervention which provides timely, comprehensive, and accessible screening for diabetes complications to people experiencing homelessness. We will assess the reach, effectiveness, adoption, implementation, and maintenance, as per the RE-AIM framework, of a SAFER model of care (i.e., screening for A1C, feet, eyes, and renal function). A trained nurse will provide this screening within a homeless shelter. During these encounters, eligible participants will be screened for microvascular complications (neuropathy, nephropathy, retinopathy) and have their A1C measured, all at the point of care, using bedside tools and novel technology. Effectiveness, our primary objective, will be evaluated using a pre-post design, by comparing the rate of completion of full microvascular screening during the study period with individuals’ own historical screening in the 2-year period prior to enrollment. The other domains of the RE-AIM framework will be assessed using process data, chart reviews, patient surveys, and qualitative semi-structured interviews with service providers and participants. This study will be conducted in a large inner-city homeless shelter within a major urban Canadian city (Calgary, Canada).

Discussion
Currently, screening for diabetes complications is often inaccessible for individuals experiencing homelessness, which places heavy burdens on individuals and, ultimately, on already strained emergency and acute care services when complications go undetected at earlier stages. The SAFER intervention will modify the current standard of care for this population in a way that is less fragmented, more person-focused, and timely, with the goal of ultimately improving the rate of screening in an acceptable fashion to identify those requiring specialist referral at earlier stages.
Digital participatory workshops with patients and health professionals to develop an intervention for the management of polypharmacy: results from a mixed-methods evaluation and methodological conclusions
Authors: Engler Jennifer , Brosse Franziska , Dinh Truc Sophia , Klein Astrid-Alexandra , Brueckle Maria-Sophie , Petermann Jenny , Muth Christiane , Mergenthal Karola , van den Akker Marjan , Voigt Karen 
Published: September 2022

Abstract
Background
In the COVID-19 pandemic, numerous researchers postponed their patient and public involvement (PPI) activities. This was mainly due to assumptions on patients’ willingness and skills to participate digitally. In fact, digital PPI workshops differ from in-person meetings as some forms of non-verbal cues and body language may be missing and technical barriers may exist. Within our project HYPERION-TransCare we adapted our PPI workshop series for intervention development to a digital format and assessed whether these digital workshops were feasible for patients, health care professionals and researchers.

Methods
We used a digital meeting tool that included communication via audio, video and chat. Discussions were documented simultaneously on a digital white board. Technical support was provided via phone and chat during the workshops and with a technical introduction workshop in advance. The workshop evaluation encompassed observation protocols, participants’ feedback via chat after each workshop on their chance to speak and the usability of the digital tools, and telephone interviews on patients’ and health professionals’ experiences after the end of the workshop series.

Results
Observation protocols showed an active role of moderators in verbally encouraging every participant to get involved. Technical challenges occurred, but were in most cases immediately addressed and solved. Participants median rating of their chance to speak and the usability of the digital tool was “very good”. In the evaluation interviews participants reported a change of perspective and mutual understanding as a main benefit from the PPI workshops and described the atmosphere as inclusive and on equal footing. Benefits of the digital format such as overcoming geographical distance, saving time and combining workshop participation with professional or childcare obligations were reported. Technical support was stressed as a pre-condition for getting actively involved in digital PPI.

Conclusions
Digital formats using different didactic and documentation techniques, accompanied by technical support, can foster active patient and public involvement. The advantages of digital PPI formats such as geographical flexibility and saving time for participants as well as the opportunity to prepare and hold workshops in geographically stretched research teams persists beyond the pandemic and may in some cases outweigh the advantages of in-person communication.
Characterization of influenza infection in a high-income urban setting in Nairobi, Kenya
Authors: Miring’u Gabriel , Muriithi Betty , Shoji Hisashi , Symekher Samwel M. L. , Wandera Ernest Apondi , Majisu Claire , Takei Mitsuo , Mwiraria Koome , Saito Yukie , Kaneko Satoshi , Tokimatsu Issei 
Published: September 2022

Abstract
Background
Influenza viruses are an important cause of respiratory infections across all age groups. Information on occurrence and magnitude of influenza virus infections in different populations in Kenya however remains scanty, compromising estimation of influenza disease burden. This study examined influenza infection in an urban high-income setting in Nairobi to establish its prevalence and activity of influenza viruses, and evaluated diagnostic performance of a rapid influenza diagnostic test.

Methodology
A cross-sectional hospital-based study was conducted in six private health facilities located within high-income residential areas in Nairobi from January 2019 to July 2020. Patients of all ages presenting with influenza-like illness (ILI) were recruited into the study. Detection of influenza virus was conducted using rapid diagnosis and reverse transcription–polymerase chain reaction (RT–PCR). Data were summarized using descriptive statistics and tests of association. Sensitivity, specificity and area under receiver operating characteristics curve was calculated to establish diagnostic accuracy of the rapid diagnosis test.

Results
The study recruited 125 participants with signs and symptoms of ILI, of whom 21 (16.8%) were positive for influenza viruses. Of all the influenza-positive cases, 17 (81.0%) were influenza type A of which 70.6% were pandemic H1N1 (A/H1N1 2009). Highest detection was observed among children aged 5–10 years. Influenza virus mostly circulated during the second half of the year, and fever, general fatigue and muscular and joint pain were significantly observed among participants with influenza virus. Sensitivity and specificity of the diagnostic test was 95% (95% confidence interval 75.1–99.9) and 100% (95% confidence interval 96.5–100.0), respectively.

Conclusions
Findings of this study shows continuous but variable activity of influenza virus throughout the year in this population, with substantial disease burden. The findings highlight the need for continuous epidemiologic surveillance including genetic surveillance to monitor activity and generate data to inform vaccine introduction or development, and other interventions.
Enhancer RNA commits osteogenesis via microRNA-3129 expression in human bone marrow-derived mesenchymal stem cells
Authors: Nguyen Anh Phuong , Yamagata Kaoru , Iwata Shigeru , Trimova Gulzhan , Zhang Tong , Shan Yu , Nguyen Mai-Phuong , Sonomoto Koshiro , Nakayamada Shingo , Kato Shigeaki , Tanaka Yoshiya 
Published: September 2022

Abstract
Background
Highly regulated gene expression program underlies osteogenesis of mesenchymal stem cells (MSCs), but the regulators in the program are not entirely identified. As enhancer RNAs (eRNAs) have recently emerged as a key regulator in gene expression, we assume a commitment of an eRNA in osteogenesis.

Methods
We performed in silico analysis to identify potential osteogenic microRNA (miRNA) gene predicted to be regulated by super-enhancers (SEs). SE inhibitor treatment and eRNA knocking-down were used to confirm the regulational mechanism of eRNA. miRNA function in osteogenesis was elucidated by miR mimic and inhibitor transfection experiments.

Results
miR-3129 was found to be located adjacent in a SE (osteoblast-specific SE_46171) specifically activated in osteoblasts by in silico analysis. A RT-quantitative PCR analysis of human bone marrow-derived MSC (hBMSC) cells showed that eRNA_2S was transcribed from the SE with the expression of miR-3129. Knockdown of eRNA_2S by locked nucleic acid as well as treatment of SE inhibitors JQ1 or THZ1 resulted in low miR-3129 levels. Overexpression of miR-3129 promoted hBMSC osteogenesis, while knockdown of miR-3129 inhibited hBMSC osteogenesis. Solute carrier family 7 member 11 (SLC7A11), encoding a bone formation suppressor, was upregulated following miR-3129-5p inhibition and identified as a target gene for miR-3129 during differentiation of hBMSCs into osteoblasts.

Conclusions
miR-3129 expression is regulated by SEs via eRNA_2S and this miRNA promotes hBMSC differentiation into osteoblasts through downregulating the target gene SLC7A11. Thus, the present study uncovers a commitment of an eRNA via a miR-3129/SLC7A11 regulatory pathway during osteogenesis of hBMSCs.
The propensity of fomite spread of SARS-CoV-2 virus through produce supply chain
Authors: Adedeji Akinbode A. , Vijayakumar Paul Priyesh 
Published: September 2022

Abstract
Background
The global community has battled the spread of SAR-CoV-2 for almost 2 years, and the projection is that the virus may be recurrent like the seasonal flu. The SARS-CoV-2 pandemic disrupted activities within the food supply chain that cost billions of dollars globally. This has heightened concerns about fomite spread of the virus through surfaces. There is an urgent need to understand the risk portends by this virus along the produce supply chain with conditions (low temperature and high relative humidity) conducive to extended survival of the virus.

Main body
Pre-dating SARS-CoV-2 are other types of coronaviruses that had lower infection and mortality rates. There are some similarities between the former and the new coronavirus, especially with regards to transmission modes and their survivability on surfaces. There is evidence of other coronaviruses’ survival on surfaces for weeks. Currently, there are limited evidence-based studies to enlighten us on how the virus is transmitted within the produce supply chain. A few studies claim that the virus could spread through the cold supply chains. However, these are not sufficient to make a conclusive inference about the deadly SARS-CoV-2.

Conclusions
This paper provides a succinct review of the literature on current understanding of the transmission, survivability, and risk SARS-CoV-2 portend to humans within the produce supply chain and calls for more evidence-based research to allay or alert us of the potential risk of fomite transmission of SARS-CoV-2. The paper also highlights examples of conventional and novel non-thermal inactivation and sanitation methods applicable to this type of virus.
GmPAO-mediated polyamine catabolism enhances soybean Phytophthora resistance without growth penalty
Authors: Yang Kun , Yan Qiang , Wang Yi , Peng Hao , Jing Maofeng , Dou Daolong 
Published: September 2022

AbstractPlant immunity is activated upon perception of pathogens and often affects growth when it is constitutively active. It is still a challenge to balance plant immunity and growth in disease resistance breeding. Here, we demonstrated that soybean (Glycine max) polyamine oxidase (GmPAO) confers resistance to multiple Phytophthora pathogens, but has no obvious adverse impact on agronomic traits. GmPAO produces H2O2 by oxidizing spermidine and spermine. Phytophthora sojae induces an increase in these two substrates, and thus promotes GmPAO-mediated polyamine catabolism specifically during infection. Interestingly, we found that the two substrates showed higher accumulation in transgenic soybean lines overexpressing GmPAO than in WT and CK after inoculation with P. sojae to ensure H2O2 production during infection, rather than directly inhibit P. sojae. In these transgenic soybean plants, the significantly enhanced resistance to different P. sojae isolates was achieved; PAMP-induced H2O2 accumulation was enhanced by GmPAO overexpression. Moreover, transient expression of GmPAO also significantly improved Nicotiana benthamiana resistance to Phytophthora capsici and Phytophthora parasitica in agroinfiltration assays. Our results provide a novel approach to allow rapid defense responses in plants upon pathogen infection while minimizing growth penalties under normal conditions, with a clear mechanism in which plant promotes H2O2 production via pathogen-activated substrates.
Efficient isolation of keratin from protein-rich waste biomass: a practical approach to minimize environmental impact and valorize waste biomass
Authors: Maity Tapan Kumar , Singh Nripat , Vaghela Pradipkumar , Ghosh Arup , Singh Sanju , Shinde Pramod B. , Sequeira Rosy Alphons , Prasad Kamalesh 
Published: September 2022

AbstractAmong various protein-containing biomass wastes, waste animal wool, poultry feather, and human hair are considered one of the most important renewable sources of keratin. Animal wool and human hair are utilized for the production of several products. However, the substantial quantity of short fibers that are inappropriate for spinning and being unusable is thrown away as waste resulting in significant environmental issues in terms of their accumulation in water bodies resulting in obstruction of waterways and other related problems. Similarly, poultry wastes, especially waste chicken feathers (WCF) are dumped or burnt or used as low-value fertilizer in certain applications. The purpose of this research is to develop an efficient method that can extract the recoverable keratin from various wastes and effectively utilize the spent solvent in the extraction process. Herein suitability of an aqueous solution of quaternary ammonium hydroxide known as tetramethyl ammonium hydroxide (TMAOH, 25% w/w in water) to solubilize these protein wastes and extract keratin from them was investigated. The solvent could solubilize ca. 39–44% w/w of waste animal wool (WAW), 19–25% of waste human hair (WHH), and 55–60% of WCF. Crude keratin with ca. 19–20%, 35–37%, and 69–74% were isolated from WAW, WHH, and WCF, respectively. The chemical and structural stability of keratin thus isolated was established. The recovered TMAOH, insoluble WAW, and WCF were found to be nontoxic to soil microbes. The recovered TMAOH thus generated after isolation of keratin was used for green gram (Vigna radiata) seed treatment, and a substantial increase in the height (4–12%) and weight (9–58%) of the plants was observed. Treating biomass waste as a source of high-value compounds may minimize environmental impact by reducing the waste load.
Job attributes affect the relationship between perceived overqualification and retention
Authors: Piotrowska Maria 
Published: September 2022

AbstractThe paper explores the possibility of reducing the effect of perceived overqualification on employee retention (i.e., turnover intentions and job search behaviors) through non-salary (nonwage benefits, elasticity of work hours, and procedural justice) and salary (pay satisfaction) attributes of work. The problem of overqualification arises when the skills and experience or the knowledge and education of an employee are higher than those required for the job that the employee performs. This situation may induce an employee to leave the organization. This research uses the concept of perceived overqualification and addresses three unresolved issues regarding salary and non-salary job attributes which can modify the effects of perceived overqualification. These issues include the mechanism through which the aforementioned effects are transferred onto retention, interpersonal justice as a moderator, and the importance of negative affectivity, which may be responsible for the relationship between perceived overqualification and employee retention. The study uses conditional analysis of the process developed by Hayes and data from a survey conducted among 100 overqualified employees, who were identified among 826 randomly selected people in Poland. The findings show that non-salary job attributes can be a better instrument than salary in increasing the organizational commitment perceived by overqualified employees. A stronger organizational commitment prevents the overqualified from leaving the organization. As regards the relationship between perceived overqualification and turnover intention, the moderation impact of interpersonal justice (respect from supervisors) is stronger than that of pay satisfaction. Negative affectivity does not create the common tendency in perceived overqualification and retention. Based on the findings, I propose several practical recommendations.