bluuebunny/crossref_metadata_2025_split huggingface dataset:
Melatonin decreases plasma TNF‐α and improves nonenzymatic antioxidant defence and insulin sensitivity in rats with apical periodontitis fed a high‐fat diet
Authors: dos Santos Rodrigo Martins , Tsosura Thais Verônica Saori , Belardi Bianca Elvira , Chaves‐Neto Antonio Hernandes , Chiba Fernando Yamamoto , Mattera Maria Sara de Lima Coutinho , Tessarin Gestter Willian Lattari , Bravo Lara Teschi , Cintra Luciano Tavares Angelo , Matsushita Dóris Hissako 
Published: October 2022

AbstractAimTo analyse the effects of melatonin (ME) treatment on oxidative stress and insulin resistance (IR) in rats with apical periodontitis (AP) fed a high‐fat diet (HFD).MethodologyEighty 60‐day‐old rats were divided into eight groups: control (CN), AP, HFD with AP (HFDAP), control with ME (CNME), AP with ME (APME), HFD with ME (HFDME) and HFD with AP+ME (HFDAPME). The animals from the HFD groups were fed a HFD throughout the experimental period. On day 7, the animals from the AP groups were subjected to experimental AP, and after 70 days, the ME groups were treated for 30 days. Glycaemia, insulinaemia, homeostatic model assessment for IR index, tumour necrosis factor‐α (TNF‐α), and interleukin‐6 were analysed in plasma using biochemical tests and enzyme‐linked immunosorbent assay. Thiobarbituric acid‐reactive substances (TBARS), carbonyl protein (CP), superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione (GSH) and total antioxidant capacity (ferric reducing antioxidant power [FRAP]) were analysed in the gastrocnemius muscle.Results(1) Association of AP and HDF exacerbated IR, and ME treatment improved this alteration; (2) AP and HFD and their association showed increased TNF‐α, and ME reversed it; (3) TBARS increased in the AP and HFDAP groups, and ME reversed only in the group with the association of disease and diet; (4) CP increased in all HFD groups and improved in the ME groups; (5) GSH activity decreased in all experimental groups, and ME increased this parameter only in the CN and AP groups; (6) FRAP did not change between the groups, but ME treatment increased its activity in the AP and HFD groups; (7) ME increased SOD in the CN and AP groups.ConclusionApical periodontitis and HFD promoted IR, and the association of AP with diet promoted IR exacerbation; this resistance might have been caused by an increase in TNF‐α. AP promoted more intense changes in lipid oxidative damage than in protein oxidative damage. In non‐enzymatic antioxidant defence, it was observed that both AP and HFD and their association promoted a decrease in GSH levels. Overall, ME treatment reversed changes such as oxidative stress and IR.
METTL14 promotes migration and invasion of choroidal melanoma by targeting RUNX2 mRNA via m6A modification
Authors: Zhang Xi , Zhang Xiaonan , Liu Tengyue , Zhang Zhe , Piao Chiyuan , Ning Hong 
Published: October 2022

AbstractThe modification of N6‐methyladenosine is involved in the progression of various cancers. This study aimed to clarify its regulatory mechanism in the pathogenesis of choroidal melanoma. Expression of methyltransferase‐like 14 in choroidal melanoma or normal choroidal tissues was determined by Western blot and immunohistochemistry. The impacts of methyltransferase‐like 14 on invasion and migration of choroidal melanoma cells were determined using functional and animal experiments. The interaction between methyltransferase‐like 14 and its downstream target was identified by methylated RNA immunoprecipitation and a dual‐luciferase reporter assay. Additionally, Wnt/β‐catenin signalling pathway was evaluated by Western blot. Methyltransferase‐like 14 was upregulated in choroidal melanoma compared to the normal choroidal tissues. Overexpression or knockdown of methyltransferase‐like 14 enhanced or inhibited the invasion and migration of choroidal melanoma cells, respectively, both in vivo and in vitro. Methyltransferase‐like 14 directly targeted downstream runt‐related transcription factor 2 mRNA, depending on N6‐methyladenosine. Additionally, the Wnt/β‐catenin signalling pathway was activated by methyltransferase‐like 14 in choroidal melanoma cells. Our study identified a novel RNA regulatory mechanism in which runt‐related transcription factor 2 was upregulated by enhanced expression of methyltransferase‐like 14 via N6‐methyladenosine modification, thus facilitating migration and invasion of choroidal melanoma cells.
A new isolate of Streptomyces lateritius (Z1‐26) with antibacterial activity against fish pathogens and immune enhancement effects on crucian carp (Carassius auratus)
Authors: Yang Yahui , Jin Duo , Long Wensu , Lai Ximiao , Sun Yunjun , Zhai Feng , Wang Pan , Zhou Xixun , Hu Yibo , Xia Liqiu , Yi Ganfeng 
Published: October 2022

AbstractThe Streptomyces lateritius Z1‐26 was isolated from soil samples which showed broad‐spectrum antibacterial activity against a broad range of fish pathogens. The In Vivo Imaging System (IVIS) monitored that strain Z1‐26 could survive and colonize in the gills and abdomen of crucian carp. The effects of dietary supplementation with strain Z1‐26 were evaluated with respect to the growth performance, antioxidant capacity, and immune response of crucian carp. The results showed that the Z1‐26‐fed fish had a significantly higher growth rate than the fish fed the control diet. The immune and antioxidant parameters revealed that the non‐specific immune indicators (AKP, SOD, and LZM) of the serum, the expression of immune‐related genes (IgM, C3, and LZM), and antioxidant‐related genes (Nrf2 and Keap1) of the immune organs were significantly increased, whereas the expression of pro‐inflammatory factors (IL‐1β, IL‐8, and TNF‐α) of the immune organs was significantly down‐regulated in crucian carp fed strain Z1‐26 compared with fish fed a control diet. Moreover, fish fed with Z1‐26 supplemented diets showed a significantly improved survival rate after Aeromonas hydrophila infection. In addition, the whole genome analysis showed that strain Z1‐26 possesses 28 gene clusters, including 6 polyketide synthetase (PKS), 4 non‐ribosomal peptide‐synthetase (NRPS), 1 bacteriocin, and 1 lantipeptide. In summary, these results indicated that strain Z1‐26 could improve the growth performance and disease resistance in crucian carp, and has the potential to be developed as a candidate probiotics for the control of bacterial diseases in aquaculture.
Ultrasound‐assisted extraction of mango seed kernel butter and assessment of its physicochemical, thermal, and structural properties
Authors: Kaur Brahmeet , Panesar Parmjit Singh , Anal Anil Kumar 
Published: October 2022

AbstractMango is an important tropical fruit with worldwide consumption and acceptance; however, the production of a large volume of waste in the form of mango seed kernels after the processing of mango has been a menace to food processing industries. Extraction of mango seed kernel butter from mango seed kernel can be an effective solution for the valorization of these by‐products. Among all the green techniques tested in the present studies, ultrasound‐assisted extraction gave the highest yield of mango seed kernel butter (11%) at 25°C, 1:20 solid–liquid ratio, 30% amplitude after a treatment time of 10 min. Physicochemical, structural, and thermal profiles of the extracted mango seed kernel butter revealed a comparatively superior quality of fat obtained using the ultrasonication technique as compared to the conventional method. Peroxide value, acid value, and antioxidant activity of mango seed kernel butter obtained using ultrasonication were found to be 2.8 meq/kg, 3.81 mg KOH/g, and 30.7%, respectively. Mango seed kernel butter, being a natural antioxidant, can serve as a promising substitute to edible oils and as a functional food ingredient in bakery and confectionery.Practical applicationsValorization of mango seed kernels for the extraction of mango seed kernel butter offers a sustainable and economical solution for the effective management of by‐products generated after mango processing. The application of ultrasonication can prove to be an effective green technique for the extraction of mango seed kernel butter with a higher yield obtained with lesser time and solvent consumption than conventional techniques. The ultrasonically extracted mango seed kernel butter exhibited better physicochemical and structural attributes than the conventional method without causing any negative impact on the nutritional profile of the obtained fat. The extracted mango seed kernel butter can be utilized as a functional food ingredient in applications of bakery and confectionary industries.
IQ67 DOMAIN protein 21 is critical for indentation formation in pavement cell morphogenesis
Authors: Feng Xinhua , Pan Shujuan , Tu Haifu , Huang Junjie , Xiao Chuanlei , Shen Xin , You Lei , Zhao Xinyan , Chen Yongqiang , Xu Danyun , Qu Xiaolu , Hu Honghong 
Published: October 2022

AbstractIn plants, cortical microtubules anchor to the plasma membrane in arrays and play important roles in cell shape. However, the molecular mechanism of microtubule binding proteins, which connect the plasma membrane and cortical microtubules in cell morphology remains largely unknown. Here, we report that a plasma membrane and microtubule dual‐localized IQ67 domain protein, IQD21, is critical for cotyledon pavement cell (PC) morphogenesis in Arabidopsis. iqd21 mutation caused increased indentation width, decreased lobe length, and similar lobe number of PCs, whereas IQD21 overexpression had a different effect on cotyledon PC shape. Weak overexpression led to increased lobe number, decreased indentation width, and similar lobe length, while moderate or great overexpression resulted in decreased lobe number, indentation width, and lobe length of PCs. Live‐cell observations revealed that IQD21 accumulation at indentation regions correlates with lobe initiation and outgrowth during PC development. Cell biological and genetic approaches revealed that IQD21 promotes transfacial microtubules anchoring to the plasma membrane via its polybasic sites and bundling at the indentation regions in both periclinal and anticlinal walls. IQD21 controls cortical microtubule organization mainly through promoting Katanin 1‐mediated microtubule severing during PC interdigitation. These findings provide the genetic evidence that transfacial microtubule arrays play a determinant role in lobe formation, and the insight into the molecular mechanism of IQD21 in transfacial microtubule organization at indentations and puzzle‐shaped PC development.
Role of estrogen in women's Alzheimer's disease risk as modified by APOE
Authors: Valencia‐Olvera Ana C. , Maldonado Weng Juan , Christensen Amy , LaDu Mary Jo , Pike Christian J. 
Published: October 2022

AbstractAlzheimer's disease (AD) is characterized by numerous sexual dimorphisms that impact the development, progression, and probably the strategies to prevent and treat the most common form of dementia. In this review, we consider this topic from a female perspective with a specific focus on how women's vulnerability to the disease is affected by the individual and interactive effects of estrogens and apolipoprotein E (APOE) genotype. Importantly, APOE appears to modulate systemic and neural outcomes of both menopause and estrogen‐based hormone therapy. In the brain, dementia risk is greater in APOE4 carriers, and the impacts of hormone therapy on cognitive decline and dementia risk vary according to both outcome measure and APOE genotype. Beyond the CNS, estrogen and APOE genotype affect vulnerability to menopause‐associated bone loss, dyslipidemia and cardiovascular disease risk. An emerging concept that may link these relationships is the possibility that the effects of APOE in women interact with estrogen status by mechanisms that may include modulation of estrogen responsiveness. This review highlights the need to consider the key AD risk factors of advancing age in a sex‐specific manner to optimize development of therapeutic approaches for AD, a view aligned with the principle of personalized medicine.
The development of the Standardised Tool for the Assessment of Bruxism (STAB): An international road map
Authors: Manfredini Daniele , Ahlberg Jari , Aarab Ghizlane , Bracci Alessandro , Durham Justin , Emodi‐Perlman Alona , Ettlin Dominik , Gallo Luigi M. , Häggman‐Henrikson Birgitta , Koutris Michail , Peroz Ingrid , Svensson Peter , Wetselaar Peter , Lobbezoo Frank 
Published: October 2022

AbstractThis paper summarises the background reasoning and work that led to the selection of the items included in the Standardised Tool for the Assessment of Bruxism (STAB), also introducing the list of items. The instrument is currently being tested for face validity and on‐field comprehension. The underlying premise is that the different motor activities included in the bruxism spectrum (e.g. clenching vs. grinding, with or without teeth contact) potentially need to be discriminated from each other, based on their purportedly different aetiology, comorbidities and potential consequences. Focus should be on a valid impression of the activities' frequency, intensity and duration. The methods that can be used for the above purposes can be grouped into strategies that collect information from the patient's history (subject‐based), from the clinical assessment performed by an examiner (clinically based) or from the use of instruments to measure certain outcomes (instrumentally based). The three strategies can apply to all aspects of bruxism (i.e. status, comorbid conditions, aetiology and consequences). The STAB will help gathering information on many aspects, factors and conditions that are currently poorly investigated in the field of bruxism. To this purpose, it is divided into two axes. Axis A includes the self‐reported information on bruxism status and potential consequences (subject‐based report) together with the clinical (examiner report) and instrumental assessment (technology report). Axis B includes the self‐reported information (subject‐based report) on factors and conditions that may have an etiological or comorbid role for bruxism. This comprehensive multidimensional assessment system will allow building predictive model for clinical and research purposes.
Dental floss ties for rubber dam isolation: A proposed classification and a new technique
Authors: Alkhatib Osama A. , Bissasu Sami , Daud Alaa 
Published: October 2022

AbstractRubber dam application has become an essential part of restorative dentistry. In late 2019, dentistry faced a new challenge with the COVID‐19 pandemic and therefore, the implementation of extra isolation methods became crucial. This article introduces a classification of dental floss ties used with a rubber dam, including the “simple” ties that are subdivided into traditional ties, single‐loop self‐ligating ties and double‐loop self‐ligating ties, and a new state‐of‐the‐art design. The “compound tie” design incorporates a combination of one or more subdivisions of the simple ties. This new design may provide better isolation and more consistent gingival tissue retraction. Furthermore, due to the advanced technique applied, the tie will offer improved stability and prevent the sheet from sliding over the dental floss tie from all surfaces.
Collaborative Strategies to Improve Nutrition Security and Education: Lessons Learned During a Pandemic
Authors: Rosales Ashley , Young Shannan , Mendez Tracy , Shelden Kristal , Holdaway Megan 
Published: October 2022

ABSTRACTBACKGROUNDThe COVID‐19 pandemic intensified disparities for underserved populations as accessing resources became more difficult. Dairy Council of California launched the Let's Eat Healthy initiative to address nutrition security through collaborative solutions in the school environment.IMPLICATIONS FOR SCHOOL HEALTH POLICY, PRACTICE, AND EQUITYTo ensure nutrition security for children and families, nutritious food and nutrition education must go hand‐in‐hand. Improving access to high quality food can help address the health disparities that exist for people who are at increased risk for food insecurity. Nutrition education supports students' holistic learning and social and emotional learning skills. Nutrition education models must be increasingly flexible in the face of ongoing challenges. Collaborative efforts to connect food access hubs, such as schools, with support and resources to provide evidence‐based nutrition education and agricultural literacy can equip individuals and communities with the knowledge, skills, and ability to make nutrient‐rich food choices.CONCLUSIONSInvestments and strategies in nutrition security that utilize the Individual plus Policy, System, and Environmental (I + PSE) model, such as the Let's Eat Healthy initiative, will effectively influence positive behavior change and improve community health. Navigating challenges in a rapidly changing environment requires people and organizations to work together, across disciplines, to leverage knowledge, experience, resources, expertise, and creative thinking. Improving access to healthy food and nutrition education will be most effective when done through collaboration.
The purity of impure public goods
Authors: Brumme Anja , Buchholz Wolfgang , Rübbelke Dirk 
Published: October 2022

AbstractIn this paper, we provide a new perspective on the links between the analysis of the voluntary provision of pure and impure public goods. In particular, it is shown that the impure public good model can be transformed into a pure public good one. This innovative method not only leads to new comparative statics results, but also provides new insights on the impure public good model, for example, on causes of the nonneutrality of income transfers with regard to Nash equilibria in the impure public good case.
Organophosphate pesticide exposure and biomarkers of liver injury/liver function
Authors: Li Wei , Xiao Haitao , Wu Hong , Xu Xuewen , Zhang Yange 
Published: October 2022

AbstractBackground and AimsThere is little epidemiological evidence linking the exposure of organophosphate pesticides (OPs) to liver function or liver injury in the general population. We used data from the National Health and Nutrition Examination Survey 1999–2012 to investigate the relationship of urinary OPs with biomarkers of liver function/liver injury.MethodsThe exposures were the concentrations of urinary OP metabolites (dimethyl phosphate [DMP], dimethyl thiophosphate [DMTP], diethyl phosphate [DEP] and diethyl thiophosphate [DETP]). The health outcomes were biomarkers of liver function/liver injury. The multivariable linear regression model, restricted cubic splines (RCSs) analysis and weighted quantile sum (WQS) regression were used to evaluate the relationship between individual or overall exposure of OPs and outcomes.ResultsRegressions of RCSs suggested linear and positive associations of OP metabolites with aspartate aminotransferase/alanine aminotransferase (AST/ALT) ratio (DMP and DEP) and fibrosis‐4 (FIB‐4) index (DMP, DEP and DMTP) (all p–non‐linear values >.05). However, L‐shaped relationships were found between OP metabolites (DMTP and DETP) and blood albumin and total protein (TP) concentrations (both p and non‐linear values <.05). The positive associations of urinary DMP, DEP and DMTP with AST/ALT ratio, and with FIB‐4 score were more pronounced among non‐smokers than smokers, among alcohol drinkers than non‐drinkers and among those with a body mass index (BMI) of ≥25 than participants with a BMI of <25. However, most of the interaction p values were more than .05, indicating no significant interactions between covariates and OPs on outcomes mainly including AST/ALT, FIB‐4, ALB and TP levels. Finally, the WQS indices were positively associated with AST/ALT ratio (p = .014) and FIB‐4 score (p = .002).ConclusionsOur study added novel evidence that exposures to OPs might be adversely associated with the biomarkers of liver function/liver injury. These findings indicated the potential toxic effect of OP exposures on the human liver.
How do children with severe underweight and wasting respond to treatment? A pooled secondary data analysis to inform future intervention studies
Authors: Odei Obeng‐Amoako Gloria A. , Stobaugh Heather , Wrottesley Stephanie V. , Khara Tanya , Binns Paul , Trehan Indi , Black Robert E. , Webb Patrick , Mwangome Martha , Bailey Jeanette , Bahwere Paluku , Dolan Carmel , Boyd Erin , Briend André , Myatt Mark A. , Lelijveld Natasha 
Published: October 2022

AbstractChildren with weight‐for‐age z‐score (WAZ) <−3 have a high risk of death, yet this indicator is not widely used in nutrition treatment programming. This pooled secondary data analysis of children aged 6–59 months aimed to examine the prevalence, treatment outcomes, and growth trajectories of children with WAZ <−3 versus children with WAZ ≥−3 receiving outpatient treatment for wasting and/or nutritional oedema, to inform future protocols. Binary treatment outcomes between WAZ <−3 and WAZ ≥−3 admissions were compared using logistic regression. Recovery was defined as attaining mid‐upper‐arm circumference ≥12.5 cm and weight‐for‐height z‐score ≥−2, without oedema, within a period of 17 weeks of admission. Data from 24,829 children from 9 countries drawn from 13 datasets were included. 55% of wasted children had WAZ <−3. Children admitted with WAZ <−3 compared to those with WAZ ≥−3 had lower recovery rates (28.3% vs. 48.7%), higher risk of death (1.8% vs. 0.7%), and higher risk of transfer to inpatient care (6.2% vs. 3.8%). Growth trajectories showed that children with WAZ <−3 had markedly lower anthropometry at the start and end of care, however, their patterns of anthropometric gains were very similar to those with WAZ ≥−3. If moderately wasted children with WAZ <−3 were treated in therapeutic programmes alongside severely wasted children, we estimate caseloads would increase by 32%. Our findings suggest that wasted children with WAZ <−3 are an especially vulnerable group and those with moderate wasting and WAZ <−3 likely require a higher intensity of nutritional support than is currently recommended. Longer or improved treatment may be necessary, and the timeline and definition of recovery likely need review.
Demographic and environmental factors associated with the distribution of Aedes albopictus in Cameroon
Authors: Canelas Tiago , Thomsen Edward , Kamgang Basile , Kelly‐Hope Louise A. 
Published: October 2022

AbstractAedes‐transmitted arboviruses have spread globally due to the spread of Aedes aegypti and Aedes albopictus. Its distribution is associated with human and physical geography. However, these factors have not been quantified in Cameroon. Therefore, the aim was to develop an Ae. albopictus geo‐referenced database to examine the risk factors associated with the vector distribution in Cameroon. Data on the Ae. albopictus presence and absence were collated and mapped from studies in published scientific literature between 2000 and 2020. Publicly available earth observation data were used to assess human geography, land use and climate risk factors related to the vector distribution. A logistic binomial regression was conducted to identify the significant risk factors associated with Ae. albopictus distribution. In total, 111 data points were collated (presence = 87; absence = 24). Different data collection methods and sites hindered the spatiotemporal analysis. An increase of one wet month in a year increased the odds of Ae. albopictus presence by 5.6 times. One unit of peri‐urban area increased the odds by 1.3 times. Using publicly available demographic and environmental data to better understand the key determinants of mosquito distributions may facilitate appropriately targeted public health messages and vector control strategies.