bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developmental endothelial locus-1 attenuates palmitate-induced apoptosis in tenocytes through the AMPK/autophagy-mediated suppression of inflammation and endoplasmic reticulum stress
Authors: Park Tae Jun , Park Seung Yeon , Cho Wonjun , Oh Heeseung , Lee Hyun Jung , Abd El-Aty A. M. , Bayram Cemil , Jeong Ji Hoon , Jung Tae Woo 
Published: December 2022

Aims Myokine developmental endothelial locus-1 (DEL-1) has been documented to alleviate inflammation and endoplasmic reticulum (ER) stress in various cell types. However, the effects of DEL-1 on inflammation, ER stress, and apoptosis in tenocytes remain unclear. Methods Human primary tenocytes were cultured in palmitate (400 μM) and palmitate plus DEL-1 (0 to 2 μg/ml) conditions for 24 hours. The expression levels of ER stress markers and cleaved caspase 3, as well as phosphorylated 5' adenosine monophosphate-activated protein kinase (AMPK) and autophagy markers, were assessed by Western blotting. Autophagosome formation was measured by staining with monodansylcadaverine, and apoptosis was determined by cell viability assay and caspase 3 activity assay. Results We found that treatment with DEL-1 suppressed palmitate-induced inflammation, ER stress, and apoptosis in human primary tenocytes. DEL-1 treatment augmented LC3 conversion and p62 degradation as well as AMPK phosphorylation. Moreover, small interfering RNA for AMPK or 3-methyladenine (3-MA), an autophagy inhibitor, abolished the suppressive effects of DEL-1 on inflammation, ER stress, and apoptosis in tenocytes. Similar to DEL-1, 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), an activator of AMPK, also attenuated palmitate-induced inflammation, ER stress, and apoptosis in tenocytes, which 3-MA reversed. Conclusion These results revealed that DEL-1 suppresses inflammation and ER stress, thereby attenuating tenocyte apoptosis through AMPK/autophagy-mediated signalling. Thus, regular exercise or administration of DEL-1 may directly contribute to improving tendinitis exacerbated by obesity and insulin resistance. Cite this article: Bone Joint Res 2022;11(12):854–861.
Molecular crosstalk between articular cartilage, meniscus, synovium, and subchondral bone in osteoarthritis
Authors: Wang Maochun , Tan Guihua , Jiang Huiming , Liu Anlong , Wu Rui , Li Jiawei , Sun Ziying , Lv Zhongyang , Sun Wei , Shi Dongquan 
Published: December 2022

Aims Osteoarthritis (OA) is a common degenerative joint disease worldwide, which is characterized by articular cartilage lesions. With more understanding of the disease, OA is considered to be a disorder of the whole joint. However, molecular communication within and between tissues during the disease process is still unclear. In this study, we used transcriptome data to reveal crosstalk between different tissues in OA. Methods We used four groups of transcription profiles acquired from the Gene Expression Omnibus database, including articular cartilage, meniscus, synovium, and subchondral bone, to screen differentially expressed genes during OA. Potential crosstalk between tissues was depicted by ligand-receptor pairs. Results During OA, there were 626, 97, 1,060, and 2,330 differentially expressed genes in articular cartilage, meniscus, synovium, and subchondral bone, respectively. Gene Ontology enrichment revealed that these genes were enriched in extracellular matrix and structure organization, ossification, neutrophil degranulation, and activation at different degrees. Through ligand-receptor pairing and proteome of OA synovial fluid, we predicted ligand-receptor interactions and constructed a crosstalk atlas of the whole joint. Several interactions were reproduced by transwell experiment in chondrocytes and synovial cells, including TNC- NT5E, TNC- SDC4, FN1- ITGA5, and FN1- NT5E. After lipopolysaccharide (LPS) or interleukin (IL)-1β stimulation, the ligand expression of chondrocytes and synovial cells was upregulated, and corresponding receptors of co-culture cells were also upregulated. Conclusion Each tissue displayed a different expression pattern in transcriptome, demonstrating their specific roles in OA. We highlighted tissue molecular crosstalk through ligand-receptor pairs in OA pathophysiology, and generated a crosstalk atlas. Strategies to interfere with these candidate ligands and receptors may help to discover molecular targets for future OA therapy. Cite this article: Bone Joint Res 2022;11(12):862–872.
The prevalence and treatment of osteoporosis in patients undergoing total hip arthroplasty and the levels of biochemical markers of bone turnover
Authors: Watanabe Naoto , Miyatake Kazumasa , Takada Ryohei , Ogawa Takahisa , Amano Yusuke , Jinno Tetsuya , Koga Hideyuki , Yoshii Toshitaka , Okawa Atsushi 
Published: December 2022

Aims Osteoporosis is common in total hip arthroplasty (THA) patients. It plays a substantial factor in the surgery’s outcome, and previous studies have revealed that pharmacological treatment for osteoporosis influences implant survival rate. The purpose of this study was to examine the prevalence of and treatment rates for osteoporosis prior to THA, and to explore differences in osteoporosis-related biomarkers between patients treated and untreated for osteoporosis. Methods This single-centre retrospective study included 398 hip joints of patients who underwent THA. Using medical records, we examined preoperative bone mineral density measures of the hip and lumbar spine using dual energy X-ray absorptiometry (DXA) scans and the medications used to treat osteoporosis at the time of admission. We also assessed the following osteoporosis-related biomarkers: tartrate-resistant acid phosphatase 5b (TRACP-5b); total procollagen type 1 amino-terminal propeptide (total P1NP); intact parathyroid hormone; and homocysteine. Results The prevalence of DXA-proven hip osteoporosis (T-score ≤ -2.5) among THA patients was 8.8% (35 of 398). The spinal osteoporosis prevalence rate was 4.5% (18 of 398), and 244 patients (61.3%; 244 of 398) had osteopenia (-2.5 < T-score ≤ -1) or osteoporosis of either the hip or spine. The rate of pharmacological osteoporosis treatment was 22.1% (88 of 398). TRACP-5b was significantly lower in the osteoporosis-treated group than in the untreated group (p < 0.001). Conclusion Osteoporosis is common in patients undergoing THA, but the diagnosis and treatment for osteoporosis were insufficient. The lower TRACP-5b levels in the osteoporosis-treated group — that is, osteoclast suppression — may contribute to the reduction of the postoperative revision rate after THA. Cite this article: Bone Joint Res 2022;11(12):873–880.
Preoperative health-related quality of life is independently associated with postoperative mortality risk following total hip or knee arthroplasty
Authors: Clement Nick D. , Patton Robyn F. L. , MacDonald Deborah J. , Duckworth Andrew D. 
Published: December 2022

Aims The primary aim was to assess whether preoperative health-related quality of life (HRQoL) was associated with postoperative mortality following total hip arthroplasty (THA) and knee arthroplasty (KA). Secondary aims were to assess whether patient demographics/comorbidities and/or joint-specific function were associated with postoperative mortality. Methods Patients undergoing THA (n = 717) and KA (n = 742) during a one-year period were identified retrospectively from an arthroplasty register. Patient demographics, comorbidities, Oxford score, and EuroQol five-dimension (EQ-5D) were recorded preoperatively. Patients were followed up for a minimum of seven years and their mortality status was obtained. Cox regression analysis was used to adjust for confounding. Results During the study period, 111 patients (15.5%) undergoing THA and 135 patients (18.2%) undergoing KA had died at a mean follow-up of 7.5 years (7 to 8). When adjusting for confounding, the preoperative EQ-5D was associated with postoperative mortality, and for each 0.1 difference in the utility there was an associated change in mortality risk of 6.7% (p = 0.048) after THA, and 6.8% (p = 0.047) after KA. Comorbidities of connective tissue disease (p ≤ 0.026) and diabetes (p ≤ 0.028) were associated with mortality after THA, whereas MI (p ≤ 0.041), diabetes (p ≤ 0.009), and pain in other joints (p ≤ 0.050) were associated with mortality following KA. The preoperative Oxford score was associated with mortality, and for each one-point change in the score there was an associated change in mortality risk of 2.7% (p = 0.025) after THA and 4.3% (p = 0.003) after KA. Conclusion Worse preoperative HRQoL and joint specific function were associated with an increased risk of postoperative mortality. Both HRQoL and joint-specific function decline with longer waiting times to surgery for THA and KA and therefore may result in an increased postoperative mortality risk than would have been expected if surgery had been undertaken earlier. Cite this article: Bone Jt Open 2022;3(12):933–940.
Effect of absorption oscillation of resonant radiation observed in a compact installation for hyper-polarization of 129Xe
Authors: Radnatarov Daba , Kobtsev Sergey 
Published: December 2022

This work presents the discovery and study of a new, to the best of our knowledge, effect of oscillation of resonant radiation absorption in optical cells with rubidium vapor in the presence of xenon and nitrogen inside an installation for xenon polarization by spin-exchange optical pumping. This effect was observed at temperatures of 100°C–130°C, partial xenon pressure exceeding 100 kPa, total gas mix pressure of 400 kPa, and power of broad-band (

Δ
λ
=

∼
2

n
m

) laser radiation over 2 W. The highest registered magnitude of power oscillations of the transmitted radiation exceeded 20%, the oscillation period depending on the temperature and lying in the range of 4.8–7 s. The conducted measurements of the parameters of the transmitted radiation lead to the conclusion that the discovered effect is related to concentration oscillations of the atomic rubidium vapor, which probably arises due to cyclic dimerization of rubidium or an oscillating continuous chemical reaction of rubidium and the optical cell contents.
EXTERNAL DEBT AND ECONOMIC GROWTH IN GHANA: A CO-INTEGRATION AND VECTOR ERROR CORRECTION ANALYSIS
Authors: ASAFO Shuffield Seyram , MATUKA Adelajda 
Published: December 2022

This paper employed a co-integration analysis and an error correction methodology to examine the impact of external debt on economic growth in Ghana using annual time series for the period 1970-2017. We found that external debt inflows spur growth in Ghana both in the long-run and short-run. Secondly, our study also confirmed the crowding out effect and the non-linear effect of external debt in the long run and short-run. However, Debt overhang was only confirmed in the short-run.
We advocate for a judicious allocation of the debt resources so that the cost of servicing the debt will not outweigh the benefit of the borrowed funds.
Enhancement isolation antenna to multi-port for wireless communication
Authors: Rahi Kareem Dhahir , Taklef Leana Jabble , Wali Musa H. 
Published: December 2022

Abstract
Currently, there is a rapid increase in demand for the transmission of large amount of data with extreme accuracy, which is usually performed by the antenna system. Therefore, this work aims to design an antenna system that has the potential of improving the performance of such system in terms of reducing the antenna size, and expanding the bandwidth. First, a single-port antenna was designed after calculating the basic requirements and then a comprehensive analysis of the antenna performance was carried out to reach optimum performance. Then, the antenna was connected to two, three, and four ports. Such connection was implemented while maintaining efficiency and magnification in each case within the range for wireless applications. The antenna with four input ports was designed with a 45° angle on a circular circumference around the antenna to prevent interference between the ports. The modified antenna was designed to accommodate wireless communication applications (such as the 5G mobile communications network as well as the future 6G mobile communications network). The experimental results were compared with those obtained from the simulation studio Computer Simulation Technology, which is more compatible with the practical results.
Gesamtvermögensgeschäfte bei Kommanditgesellschaft und GmbH
Authors: Witt Carl-Heinz 
Published: December 2022

Abstract

Kürzlich hatte der Bundesgerichtshof zum zweiten Mal nach 2019 Gelegenheit, über die analoge Anwendung des § 179 a AktG auf andere Gesellschaften als AG und KGaA zu entscheiden, und hat eine solche Analogie neuerlich abgelehnt. Während der Senat mit der Thematik bezogen auf das GmbH-Recht vor vier Jahren erstmals befasst war, bedeutet der aktuelle Ausspruch zum KG-Recht die Aufgabe seiner gegenteiligen Rechtsprechung. Die Entscheidungen überzeugen nicht vollends, da sie im Zusammenhang mit Gesamtvermögensgeschäften den Schutz der (Minderheits-)Gesellschafter gegenüber dem Schutz des Rechtsverkehrs zu schwach gewichten. Die Verneinung der analogen Anwendung des § 179 a AktG außerhalb des Aktienrechts hat zudem neue Fragen aufgeworfen, die derzeit noch ungeklärt sind.
Increase in Tau Pathology in P290SMaptKnock-In Mice Crossed withAppNL-G-FMice
Authors: Huang Melissa , Macdonald Jennifer , Lavenir Isabelle , Chen Renren , Craxton Molly , Slavik-Smith Elizabeth , Davies Stephen W. , Goedert Michel 
Published: December 2022

AbstractAlzheimer’s Disease (AD) is characterized by the pathologic assembly of amyloid β (Aβ) peptide, which deposits into extracellular plaques, and tau, which accumulates in intraneuronal inclusions. To investigate the link between Aβ and tau pathologies, experimental models featuring both pathologies are needed. We developed a mouse model featuring both tau and Aβ pathologies by knocking the P290S mutation into murineMaptand crossing theseMaptP290Sknock-in (KI) mice with theAppNL-G-FKI line.MaptP290SKI mice developed a small number of tau inclusions, which increased with age. The amount of tau pathology was significantly larger inAppNL-G-FxMaptP290SKI mice from 18 months of age onward. Tau pathology was higher in limbic areas, including hippocampus, amygdala, and piriform/entorhinal cortex. We also observed AT100-positive and Gallyas-Braak-silver-positive dystrophic neurites containing assembled filamentous tau, as visualized byin situelectron microscopy. Using a cell-based tau seeding assay, we showed that Sarkosyl-insoluble brain extracts from both 18-month-oldMaptP290SKI andAppNL-G-FxMaptP290SKI mice were seed competent, with brain extracts from double-KI mice seeding significantly more than those from theMaptP290SKI mice. Finally, we showed thatAppNL-G-FxMaptP290SKI mice had neurodegeneration in the piriform cortex from 18 months of age. We suggest thatAppNL-G-FxMaptP290SKI mice provide a good model for studying the interactions of aggregation-prone tau, Aβ, neuritic plaques, neurodegeneration, and aging.
Localization of TRP Channels in Healthy Oral Mucosa from Human Donors
Authors: Moayedi Yalda , Michlig Stephanie , Park Mark , Koch Alia , Lumpkin Ellen A. 
Published: December 2022

AbstractThe oral cavity is exposed to a remarkable range of noxious and innocuous conditions, including temperature fluctuations, mechanical forces, inflammation, and environmental and endogenous chemicals. How such changes in the oral environment are sensed is not completely understood. Transient receptor potential (TRP) ion channels are a diverse family of molecular receptors that are activated by chemicals, temperature changes, and tissue damage. In non-neuronal cells, TRP channels play roles in inflammation, tissue development, and maintenance. In somatosensory neurons, TRP channels mediate nociception, thermosensation, and chemosensation. To assess whether TRP channels might be involved in environmental sensing in the human oral cavity, we investigated their distribution in human tongue and hard palate biopsies. TRPV3 and TRPV4 were expressed in epithelial cells with inverse expression patterns where they likely contribute to epithelial development and integrity. TRPA1 immunoreactivity was present in fibroblasts, immune cells, and neuronal afferents, consistent with known roles of TRPA1 in sensory transduction and response to damage and inflammation. TRPM8 immunoreactivity was found in lamina propria and neuronal subpopulations including within the end bulbs of Krause, consistent with a role in thermal sensation. TRPV1 immunoreactivity was identified in intraepithelial nerve fibers and end bulbs of Krause, consistent with roles in nociception and thermosensation. TRPM8 and TRPV1 immunoreactivity in end bulbs of Krause suggest that these structures contain a variety of neuronal afferents, including those that mediate nociception, thermosensation, and mechanotransduction. Collectively, these studies support the role of TRP channels in oral environmental surveillance and response.
The impact of EU identity on constitutional identities •
Authors: Popravka Lencka 
Published: December 2022

AbstractSince the Maastricht Treaty, two questions remain: what is the EU, and is there such as thing as an EU identity? Because of its specific nature, and in view of the political accession criteria, it seems there is an EU political identity. Consequently, the purpose of this article is to evaluate whether this EU political identity has had an impact on Member States' constitutional identity: because they were the latest countries to accede to the EU, but also because they were under a specific monitoring process, Bulgaria and Romania are the best examples of an EU influence, but only of a limited one.
DO PARADIGMA ASSISTENCIAL AO PARADIGMA DO DESENVOLVIMENTO INTEGRAL DA CRIANÇA: A PERCEPÇAO DE PROFESSORAS DA EDUCAÇÃO INFANTIL
Authors: Arruda Marina Patricio , Feijó de Andrade Izabel Cristina , Machado Shana Siqueira Bragaglia 
Published: December 2022

Este artigo deriva de uma dissertação de mestrado que dentre outros objetivos buscou conhecer a percepção de professoras sobre um período histórico de transição entre paradigmas educacionais. Pesquisa de caráter qualitativo foi realizada num município de médio porte de RS; tendo como instrumento de pesquisa a história oral e análise de conteúdo para tratamento dos dados colhidos em contexto de afirmação do compromisso com o desenvolvimento integral das crianças. Compreendemos, ao final do estudo que a presença das professoras no interior das creches impulsionou fortemente a transição entre o paradigma assistencial, um modelo construído historicamente nas instituições brasileiras e o paradigma de desenvolvimento integral das crianças. As professoras sentiram-se protagonistas de um tempo de mudanças. Além de cuidar, perceberam que outras dimensões acentuaram a complexidade da prática pedagógica na educação infantil como educar, brincar, interagir e associar.
Security by Design Decisions für Automatisierungssysteme
Authors: Fluchs Sarah , Tastan Emre , Mertens Martin , Ritter Jochen , Horch Alexander , Drath Rainer , Fay Alexander 
Published: December 2022

Um das Design von Automatisierungssystemen noch beeinflussen zu können, müssen Security-Entscheidungen früh während des Automation-Engineering-Workflows getroffen werden („Security by Design“). Doch die beteiligten Ingenieurinnen und Ingenieure wissen oft gar nicht, welche Security-Entscheidungen sie wann treffen müssten – oder mit ihren Design-Festlegungen implizit sowieso treffen. Das vorgestellte „Automation Security by Design Decisions“-Konzept baut auf Security- Entscheidungen als zentralem Element auf und bevorzugt Security-Maßnahmen, die durch die Nutzung systemimmanenter Eigenschaften anstatt zusätzlicher Security-Lösungen umsetzbar sind. Diese systematische Entscheidungsfindung befähigt Ingenieurinnen und Ingenieure der am Automation-Engineering-Workflow beteiligten Gewerke, „ihre“ Security-Entscheidungen auch ohne dediziertes Security-Knowhow selbst zu treffen.
An Intelligent Hybrid Model Using Artificial Neural Networks and Particle Swarm Optimization Technique For Financial Crisis Prediction
Authors: Maryam Maryam , Anggoro Dimas Aryo , Tika Muhibah Fata , Kusumawati Fitri Cahya 
Published: December 2022

Financial crisis prediction is a critical issue in the economic phenomenon. Correct predictions can provide the knowledge for stakeholders to make policies to preserve and increase economic stability. Several approaches for predicting the financial crisis have been developed. However, the classification model's performance and prediction accuracy, as well as legal data, are insufficient for usage in real applications. So that, an efficient prediction model is required for higher performance results. This paper adopts a novel two-hybrid intelligent prediction model using an Artificial Neural Network (ANN) for prediction and Particle Swarm Optimization (PSO) for optimization. At first, a PSO technique produces the hyperparameter value for ANN to fit the best architecture. They are weights and thresholds. Then, they are used to predict the performance of the given dataset. In the end, ANN-PSO generates predictions value of crisis conditions. The proposed ANN-PSO model is implemented on time series data of economic conditions in Indonesia. Dataset was obtained from International Monetary Fund and the Indonesian Economic and Financial Statistics. Independent variable data using 13 potential indicators, namely imports, exports, trade exchange rates, foreign exchange reserves, the composite stock price index, real exchange rates, real deposit rates, bank deposits, loan and deposit interest rates, the difference between the real BI rate and the real FED rate, the M1, M2 multiplier, and the ratio of M2 to foreign exchange reserves. Meanwhile, the dependent variable uses the perfect signal value based on the Financial Pressure Index. A detailed statistical analysis of the dataset is also given by threshold value to convey crisis conditions. Experimental analysis shows that the proposed model is reliable based on the different evaluation criteria. The case studies show that the result for predictive data is basically consistent with the actual situation, which has greatly helped the prediction of a financial crisis.
Effect of Robusta coffee extract gel on fibroblast and collagen during proliferative phase of IIB degree-burn on Long Evans rats
Authors: Elfiah Ulfa , Naufal Muhammad Fahmi , Hidayat Mochammad Amrun 
Published: December 2022

IIB Degree-burn takes longer to heal because only a small epithelium component remains. It requires a topical agent that can accelerate the wound healing process. One of the wound healing signs is the increased collagen formation as an extracellular matrix produced by fibroblast in the proliferative phase. This study aimed to prove the effect of Robusta coffee extract gel as a topical agent on increasing the number of fibroblasts and collagen density in IIB degree-burn treatment on Long Evans rats. This study used 24 Long Evans rats which were divided into three groups, namely positive control (silver sulfadiazine), negative control (gel base), and treatment group (2.5% of Robusta coffee extract gel). The results showed a higher number of fibroblast (p<0.05) and a denser collagen density (p<0.05) of the Robusta coffee extract gel compared to the control group on day 8 and day 14. These results indicated that the number of fibroblasts and collagen increases that can scavenge free radicals and stimulate the release of cytokines that play a role in increasing fibroblast proliferation. Robusta coffee affects expanding the number of fibroblasts and collagen density to be an alternative topical agent of second-degree burns treatment.