bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting Human Thymidylate Synthase: Ensemble-based Virtual Screening for Drug Repositioning and the Role of Water
Authors: MTEREMKO DENIS , Chilongola Jaffu , Paluch Andrew S. , Chacha Musa 
Published: June 2022

Abstract
A drug repositioning computational approach was carried to search inhibitors for human thymidylate synthase. An ensemble-based virtual screening of FDA-approved drugs showed the drugs Imatinib, Lumacaftor and Naldemedine to be likely candidates for repurposing. The role of water in the drug-receptor interactions was revealed by the application of an extended AutoDock scoring function that included the water forcefield. The binding affinity scores when hydrated ligands were docked were improved in the drugs considered. Further binding free energy calculations based on the Molecular Mechanics Poisson-Boltzmann Surface Area method revealed that Imatinib, Lumacaftor and Naldemedine scored − 130.7 ± 28.1, − 210.6 ± 29.9 and − 238.0 ± 25.4 kJ/mol, respectively, showing good binding affinity for the candidates considered. Overall, the analysis of the molecular dynamics trajectory of the receptor-drug complexes revealed stable structures for Imatinib, Lumacaftor and Naldemedine, for the entire simulation time.
Assessment of farmers’ socioeconomic and conservation practice towards watershed management in the South West of Ethiopia
Authors: Woldeab Bizuneh , Ambelu Argaw , Beyene Abebe , Efrem Zewdu , Deribe Siyoum , Megersa Moa , Alemu Tibebu , Mereta Seid Tiku 
Published: June 2022

Abstract
Background
Watershed resource conservation is important for the wellbeing of the environment and communities. Understanding farmers’ socioeconomics and watershed conservation practices help in determining necessary interventions to address environmental threats. The study area is important at regional and national level due to the hydroelectric dam of the mega power project. This study deals with the farmers’ socio economic and conservation practices of Gilgel Gibe watershed. The study targeted 305 households in 4 districts of Jimma Zone surrounding the Gilgel Gibe Reservoir I. Semi-structured questionnaire was administered via interviews and focus group discussions led by trained survey interviewers.
Result
The findings of the study indicated that majority of farmers in the watershed have adequate knowledge, positive attitude and bad practice towards watershed conservation. To achieve better means of subsistence and improve their living condition farmers are forced to put stress on natural resource, degrading watershed and increase pollution loads that affect water quality of tributaries and reservoir.
Conclusion
Based on the present scenario, we forwarded stakeholders to establish team from the elders, officials at woreda and zonal level to initiate commitment, coordinate conservation activity and promote households’ income generating activities at homestead level like conservation agriculture.
Protein arginine methyltransferase 7 suppresses migration and invasion in prostate cancer cells in vitro
Authors: Hu Ping , He Yanyan , Sun Xinyue , Chen Ping 
Published: June 2022

Abstract
Protein methyltransferase arginine 7 (PRMT7) is the sole member among protein arginine methyltransferases (PRMTs) that participates in the monomethylarginine formation involved in tumor biology, DNA damage repair, and development. In this study, we report that PRMT7 is downregulated in prostate cancer cell lines in vitro and its overexpression suppresses migration and invasion in the prostate cancer cell lines LNCaP and PC3, as determined by wound healing, migration, and invasion assays. To identify the potential downstream mediator of PRMT7, we performed whole-genome transcriptome and proteome sequencing analysis, and found that the metastasis suppressor gene KISS1R was up-regulated both at the transcriptional and translational level by PRMT7. Knockdown of KISS1R rescued the tumor-suppressive phenotype. Collectively, we uncovered the anti-oncogenic role of PRMT7 in prostate cancer cells and further demonstrated that KISS1R is a downstream effector molecule of PRMT7 in modulating tumor cell migration and invasion and PRMT7 may be effective target for the clinical treatment of prostate cancer.
RETRACTED: Improved conditionally replicative adenovirus delivery system against hepatocellular carcinoma by armed with bispecific T-cell engager
Authors: Yuan Xiangfei , Lu Yang , Yang Yuanyuan , Tian Wencong , Fan Dongmei , Liu Ruoqi , Lei Xiaomin , Xia Yafei , Yang Lei , Yan Shu , Xiong Dongsheng 
Published: June 2022

Abstract

The authors have requested that this preprint be removed from Research Square.
Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus
Authors: Zhang yufei , Yang Li , Zhang Jiali , Huang Kun , Sun Xiaomei , Yang Ying , Wang Ting , Zhang Qiang , Zou Zhong , Jin Meilin 
Published: June 2022

Abstract
BackgroundSwine influenza A virus (swIAV) is a major concern for the swine industry owing to its highly contagious nature and acute viral disease. Currently, most commercial swIAV vaccines are traditional inactivated virus vaccines. The Lactobacillus plantarum-based vaccine platform is a promising approach for mucosal vaccine development. Oral and intranasal immunisations have the potential to induce a mucosal immune response, which confers protective immunity. The aim of this study was to evaluate the probiotic potential and adhesion ability of three L. plantarum strains. Furthermore, a recombinant L. plantarum strain expressing the head domain of swIAV antigen HA1 was constructed and evaluated for its ability to prevent swIAV infection. ResultsThe three L. plantarum strains isolated from healthy pig faecal samples maintained the highest survival rate when incubated at pH 3 and at bile salt concentration of 0.3 %. They also showed high adherence to intestinal cells. All three L. plantarum strains were monitored in live mice, and no major differences in transit time were observed. Recombinant L. plantarum expressed swIAV HA1 protein (pSIP401-HA1-ZN-3) and conferred effective mucosal and systemic immune responses in the intestine as well as in the upper respiratory airways of mice. In conclusion, the oral and intranasal administration of L. plantarum strain pSIP401-HA1-ZN-3 in mice induced mucosal immunity and most importantly, provided protection against lethal influenza virus challenge. ConclusionIn summary, these findings suggest that the engineered L. plantarum strain pSIP401-HA1-ZN-3 can be considered as an alternative approach for developing a novel vaccine during an influenza A pandemic.
Modified Omar sign for the clinical diagnosis of unilateral foraminal stenosis associated with disc prolapse.
Authors: ELDANASORY OMAR 
Published: June 2022

Abstract
Objectives: A retrospective study analyses the validity of Modified Omar's Sign for the diagnosis of unilateral lumbar foraminal stenosis with disc prolapse.Methods: This study analyses 250 patients with unilateral sciatic pain, between 2011 and 2020.All the data obtained from the data base collected from our institution and our private clinics. All patients had a standardized neurological clinical assessment by applying the modified Omar test during their clinical examination, then comparing the clinical findings with the (MRI) imaging findings and comparing the preoperative clinical findings with the postoperative clinical findings. Results: As the modified Omar test was applied during the physical examination, the test was positive for all patients with positive MRI with unilateral lumbar disc prolapse with foraminal stenosis on the same side. The clinical findings also confirmed by the absence of the test after surgical intervention and after nerve root block.Conclusions: The modified Omar test is a test applied during the physical examination for specific diagnosis of lumbar foraminal stenosis of patients having unilateral lumbar disc prolapse. The correlation between clinical and radiological findings confirms the test availability. The absence of the omar sign during the test after surgical intervention, and after nerve root block is considered a sure sign of high sensitivity and specificity of the test. So, the test is a more reliable diagnostic tool for the clinical diagnosis of foraminal stenosis that can be added to the clinical examination sheet. And, for the clinical identification of the efficacy of the surgical treatment and follow-up.
Band degeneration and evolution in nonlinear triatomic chain superlattices
Authors: Gong Chen , Fang Xin , Cheng Li 
Published: June 2022

Abstract
Nonlinear superlattices exhibit unique features allowing for wave manipulations. Despite the increasing attention received, the underlying physical mechanisms and the evolution process of the band structures and bandgaps in strongly nonlinear superlattices remain unclear. Here we establish and examine strongly nonlinear superlattice models (three triatomic models) to show the evolution process of typical nonlinear band structures based on analytical and numerical approaches. We find that the strongly nonlinear superlattices present particular band degeneration and bifurcation, accompanied with the vibration mode transfer in their unit cells. The evolution processes and the physical mechanisms of the band degeneration in different models are clarified with the consideration of the mode transfer. The observed degeneration may occur as the shifting, bifurcating, shortening, merging or disappearing of dispersion curves, all depending on the arrangement of the coupled nonlinear elements. Meanwhile, the dimension of the unit cell reduces, alongside changes in the frequency range and mechanisms (Bragg and local resonance) of the bandgaps. These findings answer some foundamental questions peritinent to the study of nonlinear periodic structures, nonlinear crystals and nonlinear metamaterials, which are of interest to the broad community of physics.
Receipt of subsequent immune checkpoint inhibitors at progression among patients with solid tumors enrolled in clinical trials of adjuvant immune checkpoint inhibitors
Authors: Smith Claire E.P. , Haslam Alyson , Prasad Vinay 
Published: June 2022

Abstract
Purpose: Immune checkpoint inhibitors can induce long-term responses in metastatic cancer, thus determining how many patients on placebo control arms of adjuvant immune checkpoint inhibitors receive these drugs as standard of care at progression is critical to assess if the benefit is truly from adjuvant administration or treatment at any point. Methods: This study included recent clinical trials of adjuvant immune checkpoint inhibitors for solid tumors that gained FDA approval. We determined the number of placebo control patients with progression events, the number who actually received subsequent immune checkpoint inhibitor, and the number who were eligible to receive it. Results: Data was available from 462 placebo control patients who experienced progression in trials of adjuvant immune checkpoint inhibitors. 377 of these control patients were eligible for first line immune checkpoint inhibitors upon progression. 34% (130/377) of eligible control patients received immune checkpoint inhibitors in the first line metastatic setting. In total, 28% (130/462) received immune checkpoint inhibitors, 54% (247/462) were eligible but did not receive it, and 18% (85/462) were ineligible according to currently accepted standard of care.Conclusions: Only 34% of eligible patients in placebo control arms of trials of adjuvant immune checkpoint inhibitors for solid tumors receive these medications upon progression. This is surprisingly low, and suggests that exposure to immune checkpoint inhibitors at all, instead of in the adjuvant setting, could explain the positive effect size of recurrence free survival observed in each of these trials.
The Alteration of Intrauterine Microbiota in Chronic Endometritis Patients Based on 16s rRNA sequencing Analysis
Authors: Chen Qing , Zhang Xiaowei , Hu Qicai , Zhang Wei , Xie Yi , Wei Weixia 
Published: June 2022

Abstract
Background
Chronic endometritis (CE) is a disease of continuous and subtle inflammation occurred in the endometrial stromal area, which is often asymptomatic or present with non-specific clinical symptoms.
Methods
This study investigated the composition and distribution of the intrauterine microbiota of 71 patients who underwent hysteroscopy during the routine clinical inspection of infertility. Among them, patients who diagnosed as chronic endometritis (CE) were allocated into CE group (n = 29) and others into non-CE group (n = 42). There was no significant difference in average age between the two groups. Uterine flushing fluid were collected by the self-developed cervical trocar uterine cavity sampler and performed 16S rRNA gene sequencing.
Results
The alpha diversity in CE group was significantly higher than that in non-CE group (P < 0.05). Firmicutes were the dominant phylum in the non-CE group (72.23%), while its abundance was much lower in CE group (49.92%), but there was no statistical difference between the two groups. The abundances of Actinobacteriota and Cyanobacteria in CE group were significantly higher than in non-CE group (P < 0.05). At genus level, the abundance of Lactobacillus dominated in all samples, which presented lower abundance in CE group (40.88%) than in non-CE group (64.22%). Correspondingly, the abundance of non-Lactobacillus was higher in CE group, among which Pseudomonas and Cutibacterium increased significantly (P < 0.01). Moreover, compared with the non-CE group, the pathways involved arginine and proline metabolism (P = 0.048) and retinol metabolism (P = 0.049) were significantly enriched in CE group, while the metabolism of lipid (P = 0.028) and prenyltransferases (P = 0.046) were significantly decreased in CE group.
Conclusions
In conclusion, certain microbial community was colonized in the uterine cavity, with Lactobacillus as the dominant genera. The structure and distribution of intrauterine microbiota in CE group were different from non-CE group by showing lower abundance of Lactobacillus, and significantly higher abundance of Pseudomonas and Cutibacterium. As a result, the microbial metabolism was also altered in CE groups. This study elaborated the alteration of intrauterine microbiota in the CE patients, which would improve the diagnosis rate of CE and guiding the selection of antibiotic treatment for CE patients.
The expression of the adenosine-pathway markers CD39 and CD73 in salivary gland carcinomas harbors the potential for novel immune-checkpoint inhibition
Authors: Bauer Arthur , Gebauer Niklas , Knief Juliana , Tharun Lars , Arnold Nele , Riecke Armin , Steinestel Konrad , Witte Hanno M. 
Published: June 2022

Abstract
Background
In salivary-gland-carcinomas (SGC), there is only a small fraction of entities that appears to profit from immune-checkpoint-inhibition (ICI). Recent findings connected the activation of adenosine-signaling with a tolerogenic microenvironment. Therefore, the inhibition of adenosine-pathway markers (CD39 and/or CD73) can augment ICI and/or display a novel immunotherapeutic strategy beyond ICI. Here, we assessed the immunohistochemical expression of CD39 and CD73 across a wide spectrum of SGCs.
Methods
In total, 114 patients with SGCs consecutively diagnosed between 2001 and 2021 were assessed for clinicopathological baseline-characteristics and underwent confirmatory histopathological review. Immunohistochemical expression-levels of CD39 and CD73 were assessed by applying the tumor-proportion-score (TPS) and the immune-proportional-score (IPS) comparable to PD-L1 expression analysis in routine clinical practice. Additionally, findings were correlated with PD-L1 expression-levels.
Results
The median age was 60.6 and 51.8% patients were female. The cohort covered a spectrum of eight distinct entities. Advanced-stage disease (UICC/AJCC III/IVA-IVC) at initial diagnosis was present in the majority of patients (64/114). Immunohistochemical-staining revealed positivity for CD39 and CD73 in 48.2% and 21.1% on tumor-cells (TPS ≥ 1%) as well as 46.4% and 42.9% within the immune-cell infiltrate (IPS ≥ 1%), respectively. Further comparative analyses revealed immune-cold entities such adenoidcystic-carcinoma (AdCC), immune-hot tumors such as adenocarcinoma, not otherwise specified (AC (NOS)) and entities with intermediate immunologic features such as acinic-cell-carcinoma (ACC).
Conclusion
Current results indicate entity-specific adenosine-signaling signatures. These findings suggest that the adenosine-pathway plays a decisive role in tumor immunity among the major spectrum of SGCs. Targeting the adenosine-pathway might pose a promising therapeutic option for selected entities.
Has the COVID-19 influenced the patient presentations and therapeutic options for urolithiasis?
Authors: LUO Yijia , REN Hongyan 
Published: June 2022

Abstract
Objective: To identify the influence of COVID-19 on the patient presentations and therapeutic options of urolithiasis resort to hospital during the first year of the pandemic. Methods: A systematic literature review of Pubmed, Embase, Web of Science, Ovid EMBR, and Cochrane library was performed from Jan 2020 to Dec 2021. The PRISMA criteria were followed for article selection. The pooled analysis such as age, gender, stone characters, time of symptom to a hospital visit, therapeutic methods, and length of hospital stay was calculated using the random-effects model. A subgroup analysis of fewer than 45 days since the epidemic was conducted. Results: Twelve retrospective studies were included, and the total number of patients was 4116 patients before versus 3409 during the COVID-19. The patient decreased by 17.2% during COVID. Even with significant heterogeneity in many aspects of comparison, the sensitivity analysis showed the robustness of the results. The patients' age, gender, comorbidity, stone size, and stone location stayed unchanged. But the delay in hospital visits was statistically significant and supported by the elevated white blood count and level of hydronephrosis. Regarding therapeutic options, the rate of medical expulsive therapy & observation, shock wave lithotripsy, and definitive therapy (ureteroscopy lithotripsy, percutaneous nephrolithiasis, and bladder lithotripsy) remained unchanged. There was no death report within the included studies before or during the COVID period. The subgroup analysis of < 45 days shows the likewise results.Conclusion: The impact of the COVID-19 pandemic on patients mainly was canceling or postponing the hospital visit. However, the highest level of emergency cases was similar. Meanwhile, the essential treatment of urolithiasis remained. These demonstrate the limited influence of COVID on urolithiasis during the first year of the pandemic.
Chalcogenide films optical modification by laser influence
Authors: Olkhova Anastasiia A. , Patrikeeva Alina A. , Chapalda Evgenia N. , Sergeev Maksim M. 
Published: June 2022

Abstract

The paper presents the results of studying the effect of continuous laser exposure on the change in the photosensitivity of PbSe films in a narrow spectral range. The result of laser action on PbSe films in the mode of scanning with continuous radiation at a wavelength of 405 nm has been studied. As a result of the photothermal effect on the film structure, reflection and transmission changed in the spectral range 0.3–1.0 µm. At a power density of 121.6 W/cm2, photobleaching of the material and its melting were observed, followed by its destruction, leading to the formation of microcracks. As the power density decreased to 76 W/cm2, photodarkening of the film was observed in the region of laser action. For the modified sections of the film, in this case, a sharp decrease in transmission and reflection was observed. In terms of its optical characteristics, the material approached an absolutely dark body. This modification of the film is especially promising for the photodetection of organic molecules in the form of a gas mixture or liquid.
An Efficient Real-Time Embedded Application Mapping For NoC Based Multiprocessor System on Chip
Authors: KUMAR ARURU SAI , Rao T.V.K Hanumantha , Beechu Naresh kumar reddy 
Published: June 2022

Abstract
The evolution of the components embedded on a single chip is growing faster day by day, resulting in a considerable impact on the performance metrics and communication between the cores in the NoC architecture. So, to overcome such issues, it is important to provide an efficient mapping between the cores, such that the communication between them increases. To improve the performance of a network, throughput and latency also play a significant role. In this research paper, an efficient mapping strategy implemented on the real-time embedded applications named ERTEAM. In this algorithm, based on the minimum Core Average Distance (CAD) the mapping region is finalized, ensuring the overall mapping area reduced. The PE’s mapped according to the minimum communication energy in the selected mapping region. This research evaluated a set of embedded applications, which reveals a reduction in latency at 12.3% against BBPCR and 8.4% against SBMAP. The simulation time reduces at an average of 19% against BBPCR and 9.6% against SBMAP. The throughput increases at an average of 14.5% against BBPCR and 7.8% against SBMAP and reduces the communication energy by 15.6% against BBPCR and 5.2% against SBMAP.
Reference role of middle turbinate axilla in lacrimal sac localization assisted by computed tomographic dacryocystography
Authors: Yang Lihong , Li Hongxun , Yin Zhi , Zhang Lei , Yang Zhenhai 
Published: June 2022

Abstract
Background: The middle turbinate axilla (MTA) has always been used as a stable anatomic landmark for endoscopic surgeons to locate the lacrimal sac on the lateral nasal wall. Yet, little is known about whether the lacrimal sac size will affect the positioning effect of MTA on lacrimal sac. The aim of this study was to investigate the regularity of lacrimal sac size and lacrimal sac localization through the reference position of the MTA on computed tomographic dacryocystography (CT-DCG) images. Methods: A series of 192 endoscopic dacryocystorhinostomy (DCR) surgeries were performed. All the patients had been diagnosed as unilateral nasolacrimal duct obstruction and received CT-DCG examinations. According to the maximum transverse diameter of the lacrimal sac on CT-DCG, the patients were classified into three groups. Measurements were taken on CT-DCG parasagittal images. Results:The average distance from the sac superior fundus (SSF) to the MTA was 7.52mm±3.23mm, and it increased with the increase of the maximum transverse diameter of the sac among groups (p<0.01). The average distance from the common canaliculus (CC) to the MTA was 3.95mm±2.49mm. No significant difference among the groups (p=0.11). The average distance from the CC to the SSF was 3.41mm±1.31mm, and it increased with the increase of the sac transverse diameter among groups (p<0.01).Conclusions: The lacrimal sac can be accurately located on the lateral nasal wall by the reference position of the MTA on CT-DCG images. The distance of the SSF to the MTA and the SSF to the CC is influenced by the lacrimal sac size. The relative position of the CC to the MTA is relatively stable on CT-DCG images, which make it possible to locate the lacrimal sac of different sizes and the corresponding nasal mucosa incision design in endoscopic DCR.