bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health-related quality of life within agriculture in England and Wales: results from a EQ-5D-3L self-report questionnaire
Authors: Wheeler Rebecca , Lobley Matt 
Published: July 2022

Abstract
Background
Mental and physical health problems among the farming community are well documented but there is limited evidence regarding the overall health status of this population. This paper offers a unique insight into this issue through presenting the findings from a survey instrument, the EQ-5D-3L, which provides a standardised measure of health-related quality of life (HRQOL).

Methods
We conducted the largest ever survey (n = 15,296) of people living and working in agriculture in England and Wales to gather baseline data on health and wellbeing within this community. The survey included an assessment of HRQOL through the use of the EQ-5D-3L self-report questionnaire. A variety of statistical approaches were used to test for significant associations between HRQOL and sub-group characteristics, including the Chi-square test for independence, the Kruskal-Wallis test, and Mann-Whitney U-test. Binary logistic regression models were also created to assess the influence of a set of respondent characteristics on the likelihood of respondents reporting health problems in the EQ-5D-3L.

Results
24% of respondents reported problems with mobility; 4% reported problems with self-care; 21% reported problems with performing their usual activities; 52% reported problems with pain/discomfort; and 31% reported problems with anxiety/depression. The mean EQ-5D index value was 0.811 (median (Md) 0.796, interquartile range (IQR) 0.275). The mean self-rated health score (EQ-VAS) was 77.6 (SD 16.1) (Md 80.0, IQR 20). In general, holding other respondent characteristics equal, women reported fewer problems with mobility, self-care, performing usual activities and pain/discomfort than men, but more problems with anxiety/depression. HRQOL in the working-aged appears to be poorer among the survey population than the wider UK population.

Conclusions
Results reveal concerning levels of physical and mental health problems, especially pain/discomfort and anxiety/depression, which appear to be more prevalent within our sample than within the wider UK population. There were important gender and age-related differences in both mental and physical health. Combatting these problems through targeted support is essential for the wellbeing of the farming community and the future sustainability of UK food production.
Modeling the theory of planned behavior to predict adults’ intentions to improve oral health behaviors
Authors: Rajeh Mona Talal 
Published: July 2022

Abstract
Background
The present study aimed to apply the theory of planned behavior (TPB) to identify predictors of adults’ intentions to improve oral health behaviors.

Methods
This cross-sectional study was conducted with 1,328 adults living in the Jeddah city, Saudi Arabia. A 64-item questionnaire that evaluated behavioral intention, oral health knowledge (OHK) and TPB constructs (attitudes, perceived behavioral control, and subjective norms) was distributed. Descriptive statistics and structural equation modeling (SEM) were used to describe the data and examine the associations among the variables. A p-value of < 0.05 was considered significant.

Results
The analysis revealed that the TPB model explained 72% of the variance in oral health behavioral intentions (OHBI), indicating a good model fit. The TPB constructs of attitudes (β = 0.299), subjective norms (β = 0.035), and perceived behavioral control (β = 0.144) were significant predictors of OHBI, whereas OHK was not. Attitude was the strongest predictor of intentions to improve oral health behaviors.

Conclusions
The findings suggest that this model could be a helpful framework for designing oral health promotion and intervention programs. Such programs should focus on changing adults’ attitudes, positive influences from close relationships, and improving self-efficacy of OHB to improve their oral health behavior.
Metformin and Gegen Qinlian Decoction boost islet α-cell proliferation of the STZ induced diabetic rats
Authors: Xu Li , Jois Shreyas , Cui Hongliang 
Published: July 2022

AbstractBackgroundThe traditional Chinese medicine Gegen Qinlian Decoction (GQD), as well as metformin, had been reported with anti-diabetic effects in clinical practice.ObjectiveTo verify whether these two medicines effectively ameliorate hyperglycemia caused by deficiency of islet β-cell mass which occurs in both type 1 and type 2 diabetes.MethodsSD rats were injected with a single dose of STZ (55 mg/kg) to induce β-cell destruction. The rats were then divided into control, diabetes, GQD and metformin group. GQD and metformin groups were administered with GQD extract or metformin for 6 weeks. The islet α-cell or β-cell mass changes were tested by immunohistochemical and immunofluorescent staining. The potential targets and mechanisms of GQD and metformin on cell proliferation were tested using in silico network pharmacology. Real-time PCR was performed to test the expression of islet cells related genes and targets related genes.ResultsBoth GQD and metformin did not significantly reduce the FBG level caused by β-cell mass reduction, but alleviated liver and pancreas histopathology. Both GQD and metformin did not change the insulin positive cell mass but increased α-cell proliferation of the diabetic rats. Gene expression analysis showed that GQD and metformin significantly increased the targets gene cyclin-dependent kinase 4 (Cdk4) and insulin receptor substrate (Irs1) level.ConclusionThis research indicates that GQD and metformin significantly increased the α-cell proliferation of β-cell deficiency induced diabetic rats by restoringCdk4 and Irs1 gene expression.
Patient outcomes following AKI and AKD: a population-based cohort study
Authors: Wang Huan , Lambourg Emilie , Guthrie Bruce , Morales Daniel R. , Donnan Peter T. , Bell Samira 
Published: July 2022

Abstract
Background
Acute kidney injury (AKI) is common and associated with adverse outcomes as well as important healthcare costs. However, evidence examining the epidemiology of acute kidney disease (AKD)—recently defined as AKI persisting between 7 and 90 days—remains limited. The aims of this study were to establish the rates of early AKI recovery, progression to AKD and non-recovery; examine risk factors associated with non-recovery and investigate the association between recovery timing and adverse outcomes, in a population-based cohort.

Methods
All adult residents of Tayside & Fife, Scotland, UK, with at least one episode of community or hospital-managed AKI using KDIGO creatinine-based definition during the period 1 January 2010 to 31 December 2018 were identified. Logistic regression was used to examine factors associated with non-recovery, and Cox modelling was used to establish associations between AKI recovery timing and risks of mortality and development of de novo CKD.

Results
Over 9 years, 56,906 patients with at least one AKI episode were identified with 18,773 (33%) of these progressing to AKD. Of those progressing to AKD, 5059 (27%) had still not recovered at day 90 post AKI diagnosis. Risk factors for AKD included: increasing AKI severity, pre-existing cancer or chronic heart failure and recent use of loop diuretics. Compared with early AKI recovery, progression to AKD was associated with increased hazard of 1-year mortality and de novo CKD (HR = 1.20, 95% CI 1.13 to 1.26 and HR = 2.21, 95% CI 1.91 to 2.57 respectively).

Conclusions
These findings highlight the importance of early AKI recognition and management to avoid progression to AKD and long-term adverse outcomes.
Identification of biomarkers related to neutrophils and two molecular subtypes of systemic lupus erythematosus
Authors: Li Huiyan , Yang Pingting 
Published: July 2022

Abstract
Background
Systemic lupus erythematosus (SLE), an autoimmune disease with complex pathogenesis, poses a considerable threat to women’s health. Increasing evidence indicates that neutrophils play an important role in the development and progression of lupus.

Methods
Weighted correlation network analysis and single-sample gene set enrichment analysis (GSEA) were used to analyse SLE expression data from a comprehensive gene expression database and identify modules associated with neutrophils. Thereafter, the biomarkers most closely related to neutrophils were identified. We reclassified SLE into two molecular subtypes based on the aforementioned biomarkers and evaluated cell infiltration, molecular mechanisms, and signature pathways in each subtype.

Results
The results showed significant differences in immunological characteristics between the two molecular subtypes of SLE. Hub genes were significantly upregulated in the NEUT-H subtype, and they may be associated with lupus activity. The GSEA revealed associations between our biomarkers and key metabolic pathways.

Conclusions
Our study provides not only a classification for patients with SLE but also new cell and gene targets for immunotherapy, as well as a new experimental paradigm to explore immunotherapy for other autoimmune diseases.
Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model
Authors: Hernández Ángela-Patricia , Micaelo Ania , Piñol Rafael , García-Vaquero Marina L. , Aramayona José J. , Criado Julio J. , Rodriguez Emilio , Sánchez-Gallego José Ignacio , Landeira-Viñuela Alicia , Juanes-Velasco Pablo , Díez Paula , Góngora Rafael , Jara-Acevedo Ricardo , Orfao Alberto , Miana-Mena Javier , Muñoz María Jesús , Villanueva Sergio , Millán Ángel , Fuentes Manuel 
Published: July 2022

Abstract
Background
Nowadays, nanoparticles (NPs) have evolved as multifunctional systems combining different custom anchorages which opens a wide range of applications in biomedical research. Thus, their pharmacological involvements require more comprehensive analysis and novel nanodrugs should be characterized by both chemically and biological point of view. Within the wide variety of biocompatible nanosystems, iron oxide nanoparticles (IONPs) present mostly of the required features which make them suitable for multifunctional NPs with many biopharmaceutical applications.

Results
Cisplatin-IONPs and different functionalization stages have been broadly evaluated. The potential application of these nanodrugs in onco-therapies has been assessed by studying in vitro biocompatibility (interactions with environment) by proteomics characterization the determination of protein corona in different proximal fluids (human plasma, rabbit plasma and fetal bovine serum),. Moreover, protein labeling and LC–MS/MS analysis provided more than 4000 proteins de novo synthetized as consequence of the nanodrugs presence defending cell signaling in different tumor cell types (data available via ProteomeXchanges with identified PXD026615). Further in vivo studies have provided a more integrative view of the biopharmaceutical perspectives of IONPs.

Conclusions
Pharmacological proteomic profile different behavior between species and different affinity of protein coating layers (soft and hard corona). Also, intracellular signaling exposed differences between tumor cell lines studied. First approaches in animal model reveal the potential of theses NPs as drug delivery vehicles and confirm cisplatin compounds as strengthened antitumoral agents.
Curcumin- and resveratrol-co-loaded nanoparticles in synergistic treatment of hepatocellular carcinoma
Authors: Zheng Yongshun , Jia Ran , Li Jun , Tian Xiaohe , Qian Yeben 
Published: July 2022

AbstractBackgroundCurrently, systemic therapies for patients with advanced-stage hepatocellular carcinoma (HCC) rely mainly on systemic drugs. However, traditional systemic drugs have a high rate of serious adverse events, and the curative effects of some potential anticancer drugs, such as curcumin (CUR) and resveratrol (RSV), are less apparent due to their poor bioavailability. Therefore, it is urgent to develop a highly effective therapy to improve patient prognosis. Herein, an injectable HCC-targeted nanoparticle (NP) was designed to deliver CUR and RSV to hepatoma cells.ResultsThe molecular self-assembled NPs showed higher tumour retention through the enhanced permeability and retention (EPR) effect of the NPs and surface modification with the HCC-specific peptide moiety SP94 to effectively treat HCC. These HCC-targeted NPs led to a significant reduction in the drug dosage, delayed the rate of drug release and improved the bioavailability of the encapsulated drugs. The drug concentrations in the vicinity of the tumour increased, and a good therapeutic effect was observed without obvious side effects.ConclusionsThese SP94-mediated NPs allowed large amounts of antitumor drugs to accumulate in tumours, providing a novel strategy for innovative HCC therapy. This nanoplatform also offers an idea for exploring other potential chemotherapeutics.Graphical Abstract
Prognostic factors in colorectal liver metastases patients with various tumor numbers treated by liver resection: a single-center, retrospective study
Authors: Chen Feng-Lin , Wang Yan-Yan , Liu Wei , Xing Bao-Cai 
Published: July 2022

Abstract
Background
Multiple liver metastases is considered a risk factor for overall survival of colorectal liver metastases patients (CRLM) after curative resection. However, whether the prognostic factors were constant in patients with various liver metastases (LM) numbers has not been adequately investigated. This retrospective study aimed to evaluate the changing of prognostic factors on overall survival (OS) in CRLM patients with various LM after curative resection.

Methods
Patients who underwent liver resection for CRLM between January 2000 and November 2020 were retrospectively studied. They were divided into three subgroups according to LM numbers by X-tile analysis. Multivariable analysis identified prognostic factors in each subgroup. Nomograms were built using different prognostic factors in three subgroups, respectively. Performance of the nomograms was assessed according to the concordance index (C-index) and calibration plots. The abilities of different scoring systems predicting OS were compared by calculating the area under the time-dependent receiver operating characteristic (ROC) curve (AUC).

Results
A total of 1095 patients were included. Multivariable analysis showed tumor number increasing was an independent risk factor. Patients were subsequently divided into 3 subgroups according to the number of LM by X-tile analysis, namely solitary (n = 375), 2–4 (n = 424), and ≥ 5 (n = 296). The 3-year and 5-year OS rates were 64.1% and 54.0% in solitary LM group, 58.1% and 41.7% in 2–4 LM group, and 50.9% and 32.0% in ≥ 5 LM group, respectively (p < 0.001). In multivariable analysis, RAS mutation was the only constant independent risk factor in all subgroups. The nomograms were built to predict survival based on independent factors in three subgroups. The C-index for OS prediction was 0.707 (95% CI 0.686–0.728) in the solitary LM group, 0.695 (95% CI 0.675–0.715) in the 2–4 LM group, and 0.687 (95% CI 0.664–0.710) in the ≥ 5 LM group. The time-dependent AUC values of nomograms developed using different risk factors after stratifying patients by tumor number were higher than the traditional scoring systems without patient stratification.

Conclusions
The prognostic factors varied among CRLM patients with different LM numbers. RAS mutation was the only constant risk factor. Building prediction models based on different prognostic factors improve patient stratification.
High exposure to phthalates is associated with HbA1c worsening in type 2 diabetes subjects with and without edentulism: a prospective pilot study
Authors: Mengozzi Alessandro , Carli Fabrizia , Pezzica Samantha , Biancalana Edoardo , Gastaldelli Amalia , Solini Anna 
Published: July 2022

Abstract
Background
Phthalates exposure and complete edentulism are related to both low socioeconomic status. No study by far has verified if and to what extent these two conditions are related. We aimed to explore their potential association and interplay in the metabolic control and cardiovascular risk profile.

Methods
In our small (n = 48) prospective pilot study twenty-four patients with type 2 diabetes (DnE) and twenty-four patients with type 2 diabetes and edentulism (DE) followed for 19 ± 2 months were treated according to best clinical standards. Phthalates’ exposure was evaluated by urinary concentration of di-2-ethylhexylphthalate (DEHP), metabolites, i.e. mono 2-ethylhexyl phthalate (MEHP), mono-2-ethyl-5-oxohexyl phthalate (MEOHP) and mono 2-ethyl-5-hydroxyhexyl phthalate (MEHHP).

Results
No association between phthalates and edentulism was found, nor did edentulism affect glucose control. Higher phthalates exposure was associated with a glycated haemoglobin worsening. This association was found for all the measured phthalates metabolites, both as a whole (DEHP; r = 0.33, p = 0.0209) and individually: MEHP (r = 0.41, p = 0.0033), MEHHP (r = 0.32, p = 0.028), MEOHP (r = 0.28, p = 0.0386).

Conclusions
Phthalates are not associated with edentulism but predict the worsening of glucose control in subjects with type 2 diabetes. These findings might prove relevant in identifying novel biomarkers of cardiometabolic risk. Further studies are needed to validate our results and estimate the true potential of phthalates in terms of risk assessment.
Mesenchymal stem cell-derived exosomes protect against liver fibrosis via delivering miR-148a to target KLF6/STAT3 pathway in macrophages
Authors: Tian Siyuan , Zhou Xia , Zhang Miao , Cui Lina , Li Bo , Liu Yansheng , Su Rui , Sun Keshuai , Hu Yinan , Yang Fangfang , Xuan Guoyun , Ma Shuoyi , Zheng Xiaohong , Zhou Xinmin , Guo Changcun , Shang Yulong , Wang Jingbo , Han Ying 
Published: July 2022

Abstract
Background
Despite emerging evidence on the therapeutic potential of mesenchymal stem cells (MSCs) for liver fibrosis, the underlying mechanisms remain unclear. At present, MSC-derived exosomes (MSC-EXOs) are widely accepted as crucial messengers for intercellular communication. This study aimed to explore the therapeutic effects of MSC-EXOs on liver fibrosis and identify the mechanisms underlying the action of MSC-EXOs.

Methods
Carbon tetrachloride was used to induce a liver fibrosis model, which was intravenously administered with MSCs or MSC-EXOs to assess treatment efficacy. The resulting histopathology, fibrosis degree, inflammation and macrophage polarization were analyzed. RAW264.7 and BMDM cells were used to explore the regulatory effects of MSC-EXOs on macrophage polarization. Then, the critical miRNA mediating the therapeutic effects of MSC-EXOs was screened via RNA sequencing and validated experimentally. Furthermore, the target mRNA and downstream signaling pathways were elucidated by luciferase reporter assay, bioinformatics analysis and western blot.

Results
MSCs alleviated liver fibrosis largely depended on their secreted exosomes, which were visualized to circulate into liver after transplantation. In addition, MSC-EXOs were found to modulate macrophage phenotype to regulate inflammatory microenvironment in liver and repair the injury. Mechanically, RNA-sequencing illustrates that miR-148a, enriched in the MSC-EXOs, targets Kruppel-like factor 6 (KLF6) to suppress pro-inflammatory macrophages and promote anti-inflammatory macrophages by inhibiting the STAT3 pathway. Administration of miR-148a-enriched MSC-EXOs or miR-148a agomir shows potent ameliorative effects on liver fibrosis.

Conclusions
These findings suggest that MSC-EXOs protect against liver fibrosis via delivering miR-148a that regulates intrahepatic macrophage functions through KLF6/STAT3 signaling and provide a potential therapeutic target for liver fibrosis.
Deep eutectic solvents: green solvents for the removal of degraded gelatin on cellulose nitrate cinematographic films
Authors: Lozano Marco Valente Chavez , Sciutto Giorgia , Prati Silvia , Mazzeo Rocco 
Published: July 2022

Abstract
Cellulose nitrate (CN) has been used in the past as support for photographic negatives and cinematographic films. This material is particularly unstable and can undergoes severe degradation due to thermal, photocatalytic and hydrolytic loss of nitro groups from the lateral chain. Thus, to prevent the disappearance of the movies, their scanning and digitalization become a priority.
However, CN bases degradation may prevent the scanning of the films. The decrease in pH, for instance, lowers the viscosity of gelatin, which becomes softer. This causes the formation of gelatin residues which stick on the back of the superimposed frames inside the reels creating a deposit.
Traditional approaches to clean gelatin residues from the surface of CN bases include the mechanical removal with scalpels and the use of organic solvents (such as isopropyl alcohol). However, these methods are either slow and ineffective or could potentially damage the degraded CN supports.
To overcome these drawbacks, we have evaluated the performance of three choline chloride and betaine-based Deep Eutectic Solvent (DES) formulations as alternative for the removal of gelatine residues from CN supports. These solvents are inexpensive (when compared to traditional solvents), easy to prepare, green (non volatile, safe towards the operators and the environment, and potentially recyclable), non flammable and have been previously proposed for the extraction of proteinaceous materials, but their use for the restoration of photographic negatives or cinematographic films has not been reported yet.
Selected areas over the frames of a real deteriorated CN cinematographic film were cleaned comparing the DES performances with the ones obtained using isopropyl alcohol as an example of a traditional method.
In particular, the tested DES formulations showed superior cleaning power compared to isopropyl alcohol and, at the selected application times, resulted capable to remove the gelatin residues without affecting the CN film supports.

Graphical abstract
Influence of geomagnetic storms on the quality of magnetotelluric impedance
Authors: Chen Hao , Mizunaga Hideki , Tanaka Toshiaki 
Published: July 2022

AbstractMagnetotelluric (MT) field data contain natural electromagnetic signals and artificial noise sources (instrumental, anthropogenic, etc.). Not all available time-series data contain usable information on the electrical conductivity distribution at depth with a low signal-to-noise ratio. If variations in the natural electromagnetic signal increase dramatically in a geomagnetic storm, the signal-to-noise ratio increases. A more reliable impedance may be obtained using storm data in a noisy environment. The field datasets observed at mid-latitudes were used to investigate the effect of geomagnetic storms on MT impedance quality. We combined the coherence between the electric and magnetic fields and the result of the MT sounding curve to evaluate the MT impedance quality across all periods and combined the phase difference among the electric and magnetic fields, the polarization direction, and the hat matrix to discuss the data quality for a specific period simultaneously. The case studies showed that the utilization of the data observed during the geomagnetic storm could overcome the local noise and bring a more reliable impedance.
Graphical Abstract