bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efek Ekstrak Daun Binahong (Anredera cordifolia (Ten) Steenis) terhadap Profil Darah Merah pada Marmut (Cavia cobaya)
Authors: Wijayanti Dwi , Setiatin Enny Tantini , Kurnianto Edy 
Published: November 2022

The purpose of this study was to determine the effect of binahong leaves extract (BLE) on Cavia cobaya through the number of erythrocytes, hemoglobin, pack cell volume (PCV) and erythrocyte indices. Complete Random Design (CRD) was used in this study with 4 treatment and 4 replicate, those were T0 (control), T1 (10 mg / head / body weight), T2 (50 mg / head / body weight) and T3 (90 mg / head / weight). The administration of BLE was orally for 10 days pre partum and 10 days postpartum. Blood sampling was done three times, which wasbefore giving BLE , 1 day postpartum and 1 day after the giving of BLE. Data was analyzed using ANOVA and if there was effect of treatment, then continued with Duncan multiple range test. There was not significantly different among treatments for PCV before giving BLE, while the postpartum and post giving BLE showed significantly different (P<0.05). There was not significantl different in the percentage of Hb among treatments for postpartum while in before giving BLE and post giving BLE were showed significantly different (P<0.05). Total erythrocytes was not significantly difference among treatments for before giving BLE while in the postpartum and post giving BLE significantly different (P <0.05). MCHC value was not significantly different between treatments which was before giving BLE and post giving BLE while in the postpartum was showedsignificantly different (P<0.05). MCV and MCH values were not significantly different between treatments before giving BLE while in the postpartum and post giving BLE significantly different (P <0.05). In conclusion, giving BLE at a dose of 50 mg/head/body weight can improve immunity and endurance Cavia cobaya on the treatment group before giving BLE, postpartum and post giving BLE of PCV, Hb, total erythrocytes, MCHC, MCV and MCH.
Karakterisasi Faktor-faktor Virulensi Staphylococcus aureus Asal Susu Kambing Peranakan Ettawa secara Fenotip dan Genotip
Authors: Khusnan Khusnan , Prihtiyantoro Wahyu , Hartatik Hartatik , Slipranata Mitra 
Published: November 2022

Staphylococcus aureus is a major cause of mastitis in large or small ruminants, and often manifested by subclinical mastitis in Peranakan Ettawa (PE) goats. Staphylococcus aureus in human can cause food borne disease. The research aimed to characterize the virulence factors of Staphylococcus aureus isolated from milk PE goats, phenotypic- and genotypically. Phenotypically characterization were determined through the pigmen assay as well as hydrophobicity, haemolysin, and hemaglutinin reaction. Polymerase chain reaction (PCR) analysis was used to detect 4 virulen genes including coa, clf, fnbA, and fnbB genes. The results of research showed that Staphylococcus aureus abled to produce white pigmen (35,7%), yellow pigmen g (57,1%), andorange pigmen (7,2%). Staphylococcus aureus showed α-hemolysis zone (35,7%), β-hemolysis (35,7%), dan γ-hemolysis (28,9%). Hydrophobicytic test revealed 14,3% Staphylococcus aureus isolates were hydrophobe and85,7% hydrophil. Staphylococcus aureus (85,7%) isolates abled to aglutinated sheep blood cells. Based on genotypic analysis of Staphylococcus aureus could be detected coa gene (92,8%), clf gene (64,3%), fnbA gene (78,6%), and fnbB gene (64,3%). Based on the phenotypic and genotypic characters, it can be concluded that Staphylococcus aureus are virulent strains. This information can be used as the basis for control mastitis in PE goats
Examination of Viral Nervous Necrosis Virus in The Water Sample of The Tiger Grouper (Epinephelus fuscoguttatus) with Immunocytochemistry of Streptavidin Biotin
Authors: Sudaryatma Putu Eka , Lestari Artanti Tri , Trisnasari Yenny , Lidayana D Lia , Nurlita Wahyu 
Published: November 2022

Abstract One of potencies of marine waters that is developed, and starts to have the international market is grouper fish. Tiger grouper culture can not be separated from disease factors that may attack and destroy the cultivation. One of the important diseases that can cause mass mortality in fish, especially in grouper larvae and juveniles stadium is viral nervous necrosis (VNN). Examination by using immunocytochemical method of streptavidin biotin (SB) undertaken to develop early detection of VNN in order to be applied in preventing spread of the VNN disease in seeds of tiger groupers that are already distributed worldwide in Indonesia. And also, this SB methodology can also be applied to the tiger groupers without any injuring or even killing of the fish for the purpose of sampling. In the present study, the preliminary test was done to determine the infectivity level of the inoculum (VNN virus) owned by the Office of Fish Quarantine, Quality Control, and Fishery Safety Class I Denpasar, Bali. Whereas, the main test was done by collecting water sample from tiger grouper fish farming that was infected with VNN. The water sample was examined by using immunocytochemical method of SB after being confirmed by RT-PCR-positive test that was previously purified. Based on both SB and PCR tests having VNN positive results, it is concluded that the immunocytochemical method of SB could be applied as early, rapid and accurate detection of VNN in only a 24 hours post infection by using water as the sample. Keywords: VNN, water sample, streptavidin biotin, RT-PCR, tiger grouper
Efektivitas Larutan Desinfektan Dalam Menginaktivasi Virus Avian Influenza pada Bulu Unggas
Authors: Luwito Bagus Nanang , Wibawan I Wayan Teguh , Soejoedono Retno Damayanti 
Published: November 2022

Avian Influenza (AI) virus is pathogenic agent that can spread from one area to another area through the transportation of infected animals or their products such as feathers. This research was aimed to inactivated AI virus with 775 ppm sodium hypochlorite and 0.1% chloroxylenol to examine the difference of treatment by time (day) for inactivation AI virus on the feathers. AI virus isolate A/chicken/sidrap/ 07160336‐2/2016 used in this research was obtained from Balai Besar Veteriner Maros. The treatment of disinfectants were performed on the first day (the day of disinfectan solutions were prepared), the third day and the seventh day by soaked the feathers in disinfectant solution for 10 minutes. The effectiveness of disinfectans were evaluated by inactivation index. The result show that the average of inactivation index of 775 ppm of sodium hypochlorit was 4.17 for the first day, 5.17 for the third day, and 4.20 for the seventh day, while the average of inactivation index of 0.1% chloroxylenol was 5.50 for the first day, 6.43 for the third day, and 5.77 for the seventh day. Our result indicated that the sodium hypochlorit and chloroxylenol were effective for inactivation of AI virus. The 0.1% of chloroxylenol was more effective for inactivation AI virus than 775 ppm of sodium hypochlorit, whilst the most effective duration for the treatment is the three days.
Identifikasi Penambahan Air pada Daging Sapi dengan Metode Filter Paper Press dan Konduktivitas Listrik
Authors: Adinata Nararya , Lukman Denny Widaya , Sudarnika Etih 
Published: November 2022

Beef meat has a complete nutritional content, the high prices in the market causing some unscrupulous traders to cheat by adding water to meat to get more weight. This beef meat has a bad quality, so needs the right method to identify the quality of beef meat in the field. This study was designed to identify the addition of water to beef meat by measuring the water holding capacity (filter paper press method) and electrical conductivity (EC meter). The experimental used one hundred bovine longisimus dorsi and the samples were divided into five groups. One group as a control and four other groups were treatment group. The treatment groups were injected by water with a volume of 1%, 2%, 3%, and 4% of the sample weight and all groups were repeated for twenty times. The results were analyzed by ANOVA and Duncan Multiple Range test. The data showed that the addition of water by injection in beef meat of treatment group 2%, 3%, and 4% had a significant influence on water holding capacity and electrical conductivity (p<0.05). Pearson correlation results showed a strong to very strong correlation with value in control group (r=0.704; p<0.01), treatment group 1% (r=0.628; p<0.01), 2% (r=0.700; p<0.01), 3% (r=0.642; p<0.01) and 4% (r=0.760; p<0.01). Based on the result of the study, it is known that a water increase in beef at least 2% of the weight significantly affected water holding capacity and it can be identified by using electrical conductivity meter.
Study of The Effects of Carboxymethyl Chitosan on The Non-specific Defense System in The Carp (Cyprinus Carpio)
Authors: Hernawati Ristyana Dewi , . Triyanto , . Murwantoko 
Published: November 2022

Abstract Carp (Cyprinus carpio) is a freshwater fish with a high economic value, but very susceptible to diseases. One of effort to increase the productivity is by enhancing non-specific defense system. The purpose of this study is to determine the effect of carboxymethyl chitosan on enhancement non-specific defense system of carp. Carboxymethyl chitosan was obtained by alkylation process in which monochloroacetic acid in alkaline conditions was added. Carboxymethyl chitosan was given to carps at dosages of 30 μg/g, 75 μg/g and 105 μg/g, by intra muscular injection respectively. Seven and 14 days after administration of carboxymethyl chitosan, measurements of non-specific immune system parameters were done. The results showed that, administration of carboxymethyl chitosan on carps affected the phagocytic activity and lymphocytes counts. However, carboxymethyl chitosan did not give any effect to NBT activity, hematocrit, number of erythtocytes and leukocytes, monocytes and neutrophil counts in blood as well.
Identification of Campylobacter jejuni Using Polymerase Chain Reaction Method
Authors: Angeliya Liza , Kurdiwa Ruri Rumpaka 
Published: November 2022

Abstract Campylobacter jejuni is a curved-rod shaped bacteria, non-spore, microaerophilic Gram-negative and motile. It is commonly found in the feces of animals. They grow at a temperature of 37-42° C and are zoonotic causing a disease called campylobacteriosis. Campylobacter jejuni naturally presents in the chicken digestive tract and does not cause any diseases. However, chicken carcasses contaminated with this bacteria are closely related to the the incidence of campylobacteriosis in humans. Cases of human campylobacteriosis primarily caused by Campylobacter jejuni contamination on chicken carcasses. During the cutting process, Campylobacter jejuni will be able to spread to the chicken carcasses. Alternative approach for detecting Campylobacter jejuni contamination of chicken products is by using polymerase chain reaction (PCR) method. Therefore, to determine diagnosis of animal diseases in relation to the veterinary public health due to Campylobacter jejuni is by applying PCR with forward primer: 5'-TGACGCTAGTGTTGTAGGAG-3 and reverse primer: 5'-CCATCATCGCTAAGTGCAAC-3'. The PCR primers can amplify DNA Campylobacter jejuni as long as 402 bp. The PCR method is a molecular laboratory approach which is sensitive, specific, rapid and accurate for determining diagnosis of campylobacteriosis.
The Detection of Encoding Gene of Toxic Shock Syndrome Toxin-1 S. aureus Isolate from the Milk of Cows and Goats by Polymerase Chain Reaction
Authors: Kunda Rony M , Wahyuni A.E.T.H. , Widayanti Rini 
Published: November 2022

Abstract Staphylococcus aureus is the main bacterium found in cow's and goat's milk. The bacteria can produce toxin called toxic shock syndrome toxin-1 (TSST-1) that can infect humans and animals causing several serious diseases. The objective of this study was to detect the existence of encoding gene of TSST-1 S. aureus isolate from cow's and goat's milk. The research is initiated by re-identification stage of S. aureus conventionally continued by identification based on the molecular method of polymerase chain reaction (PCR). A number of 10 S. aureus isolates from cow's and goat's milk cultured in aerobics continued by Gram stain, catalase, coagulase, MSA, VJA and VP tests. The identification of S. aureus based on the molecular approach conducted by 16S rRNA gene amplification continued with amplification of TSST-1 encoding gene as the target gene. The PCR product of TSST-1 encoding gene then sequenced to ensure whether the DNA fragment amplified is the TSST-1 encoding gene or not. The result of the research indicates that re-identification of S. aureus conventionally generating positive reaction of S. aureus species. Molecular identification of 16S rRNA gene amplification gives a good result by producing DNA fragment of 745 bp size and meets the target gene. The detection result of encoding gene TSST-1 gives negative result marked by DNA fragment which the size does not match the target gene. The allignment result of sequence isolate SA.1 indicates that the sequence is not tst gene but the gene which coded glutamate sinthetase belongs to S. aureus, whereas sequence of isolate KI.8 is 50S rRNA gene belongs toS. saprophyticus.
The Effects of Turmeric Extract (Curcuma domestica Val) on Cigarette Smoke Cardiotoxicity in The Wistar Rats
Authors: Millah Nida Ul , Kusumawardani Agapietta , Rahmad Laelatul , Herliyani Novi , Sarwendah Kurniati , Sutrisno Bambang , Wuryastuty Hastari , Wasito R 
Published: November 2022

Abstract The objective of this research is to determine the effects of turmeric (Curcuma domestica Val) extract administration toward heart histopathologic lesions of male albino Wistar rats + 3 months old exposed by cigarette smoke. Twenty male Wistar rats were divided randomly into four groups of five each: Group I was given neither cigarette smoke exposure nor turmeric extract administration (control) (KI), Group II was given cigarette smoke exposure of 9 cigarettes/ day (KII), Group III was given 1 ml turmeric extract 0.25% (KIII) and Group IV was given cigarette smoke exposure of 9 cigarettes/ day and 1 ml turmeric extract 0.25% (KIV). After 2 months of treatment, necropsy was conducted to all rats and the heart samples were collected to be processed histopatologically and stained routinely with hematoxylin-eosin. The results of the present study indicated that the cigarette smoke caused cardiomyopathy in KII marked by the histopathologic lesions such as vacuolation, homogeneous eosinophilic cytoplasms, edema and necrosis of myocardia, also congestion and mild hemorrhages (KII). Whereas, rats in Groups KI, KIII and KIV had normal histologic structures of myocardia. It is concluded, that turmeric extract could be a preventive herbs toward cardiotoxic effects of cigarette smoke. Further research has to be conducted to determine the anti cardiomyopathy mechanism of turmeric rhizome due to cigarette smoke exposure.
The Effect of Decis Insecticide on the Mortality and Gill Histological Structure of the Red Indigo "Cangkringan local strain"
Authors: Wulandari Wahyuni , . Sukiya , . Suhandoyo 
Published: November 2022

Abstract This study was aimed to determine the effects of Decis insecticide on the mortality, its safe level concentration against mortality, and the cytotoxic effects in the gills of the red tilapia strains of "localCangkringan" collected from Fish Seed Center, Cangkringan, Sleman, Yogyakarta. Eight hundreds fishweighing 0.5-1.0 g and 2-12 weeks old each were collected randomly and divided into 5 groups with Decis insecticide concentration of 0.27 ppm, 0.81 ppm, 1.00 ppm, 1.35 ppm, 2.4 ppm, respectively and a control group based on preliminary testing. Each treatment comprised three replicates, 10 replicates of each test fish. The observations of fish mortality to the test were done every hour - 24, for 96 hours of treatment, whereas mortality at safe levels test done every 4 weeks for 2 months of treatment. Data were analyzed using probit analysis to determine LC50 and LC50-96 hours-48 hours. The assay of 10% safe levels of LC50-48 hours is 0.13 ppm. Twofactorial analysis of variance was to determine the effect of concentration and duration of exposure to the Decis insecticide test fish mortality. Independent samples of T test analysis was applied to determine the effect of safe levels. The descriptive and qualitative analysis was done to determine the pathologic lesions on fish gills due to the cytotoxicity of Decis insecticide. Results of the present study showed that the variation of concentrations of Decis insecticide has significant effect on fish mortality (p <0,01). Decis insecticide concentration used for the LD50(96 hours) was 1.21 ppm that was the highly toxic class to Nila. It caused severe cytotoxic efects to the gills indicated by the presence of gills necrosis, hypertrophy, hyperplasia dan diffuse hemorrhages. The safety levelsof Decis concentration still caused mild histopathological lesions, such as focal to multifocal hemorrhages and congestion in the gills
Validation of the Spectrophotometry method on the concetration assessment of Enrofloxacine as the Animal Drug
Authors: . Ambarwati , Ariyani Novida , Palupi Maria Fatima 
Published: November 2022

Abstract Validation for analysis of enrofloxacin injection method using spectrophotometry has been conducted. Enrofloksasin in a solution of NaOH (sodium hydroxide) 0.1 N, measured at the maximum wavelength (ë) 271 nm.The parameters of validation that were carried out are precision, accuracy, linearity, limit of detection and limit of quantitation. Precision showed good results with coefficien variation (CV) results smaller than that of CV Horwitz. Accuracy parameters calculated by the method of analysis to determine levels of analyte in a known active ingredient purity and yielded 0.449%. Linearity parameters showed good results with r = 0.9996. Limit of detection value was obtained for 0.757 μg / mL and limit of quantification of 2.522 μg / mL. The results of validation test of enrofloxacin injection analysis using spectrophotometric method can be used as a good method to provide a valid test of quality assurance veterinary drugs.
Kombinasi One-step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) dan Nucleic Acid Lateral Flow (NALF) sebagai Metode Deteksi Gen env-tm Virus Jembrana Strain Tabanan 1987
Authors: Kusumawati Asmarani , Fatimah Fatimah 
Published: November 2022

Jembrana disease is an acute viral disease in Bali cattle that have short incubation period, high mortality and morbidity rate. Early diagnostic methods are needed to prevent the further spreading of this disease. In this study, we combined One-step RT-PCR and NALF methods to detect env-tm gene of Jembrana virus Tabanan 1987 strain. Viral RNA isolated from a spleen of the infected cattle was used as the template. One-step RT-PCR procedure was performed using oligoprobes labeled digoxigenin and right primer sequence that were designed using the primer3plus program based on the conserves region of env-tm gene from NCBI database. The product of one-step RT-PCR was tested using NALF method instead of electrophoresis. Positive result was shown by the appearance of dark lines on the test line of digoxigenin in NALF dipstick device.
Pengaruh fasciolosis pada sapi bali berdasarkan pemeriksaan darah, serum glutamic pyruvic transaminase (SGPT), dan alkaline phosphatase (ALP)
Authors: Amirullah Amirullah , AS Dradjat , M Sriasih , Maskur Maskur , SN Depamede , D Kisworo , Kurniasih Kurniasih 
Published: November 2022

Investation of Fasciola sp. On several species of cattle in Indonesia has occurred since a long time ago. Fasciolosis caused a high economical loss such as decreasing of carcass and organs, sometimes caused death of calves. The objectives of the research were to evaluate the effect of fasciolosis on Serum Glutamic Pyruvic Transaminase (SGPT), and the obstruction of bile duct by Alkaline Phosphatase (ALP) in Bali cattle suffered from fasciolosis. Faeces of Bali cattle raised in endemic areas of fasciolosis were examinated by sedimentation test to identify eggs per gram of faeces (EPG) of Fasciola sp. The blood samples of cattle which positive to fasciolosis were use to study the hematology by autoanalyser, SGPT and ALP. The results of research showed that from 89 cattle, 28 were infected by Fasciola sp. whithin of 7-115 EPG (min-max). From 20 fasciolosis cattle were indicated that MCHC of 90%, RBC of 30%, HB of 20%, and eosinophils of 2 % of cattle were lower than that of normal standard, while lymphocytes of 60%, MCV of 40%, ALP of 10% and SGPT of 5% of cattle were higher than that of normal standard. hematological appearances of cattle suffered from fasciolosis had macrocytichypochromic anemia, eosinopenia, and lymphocytosis. The increase of SGPT may be caused byhepatic cells destruction by young liver flukes, and the increase of ALP was caused by the obstruction of bile duct by mature liver flukes.
Ovulatory Follicular Dynamics After Estrus Synchronization using Prostaglandin F2a in Dairy Cows
Authors: Putro Prabowo Purwono , Kusumawati Asmarani 
Published: November 2022

The study aimed to follow development of ovulatory follicular dynamics as well as plasma progesterone profile after estrus synchronization using PGF2 and GnRH. A total of 15 non-pregnant dairy cows, 4-5 years of age, healthy and reproductively sound were used in the present study. Treatment 1, given intramuscular injection of PGF2 25 mg (PGF2), treatment 2 PGF2 25 mg and GnRH 250 g 2 days later (PGF2-GnRH), and treatment 3 with GnRH 250 g (7 days prior to injection of PGF2), PGF2 25 mg and GnRH 250 g (2 days after injection of PGF2) (GnRH-PGF2a-GnRH) (the Ovsynch method). Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz tranducer was performed everyday for 12 days to follow ovulatory follicular and luteal dynamics. Blood plasma was taken every day for progesterone determination using EIA technique. Data of follicular, luteal development and progesterone levels were tested using analysis of variance and correlation analysis. The animals showed estrus within 70.70 + 01.90 hours following PGF2 injection. Prostaglandin F2 induced corpus luteum regression, decreased in progesterone plasma levels, followed by ovulatory follicular development and eventually underwent ovulation. Administration of first GnRH increased corpus luteum size, enhanced its regression and decreased plasma progesterone levels, while the second administration induce better ovulatory follicular development. Rate of the corpus luteum regression, progesterone decrease and ovulatory follicular development following PGF2 injection for respective treatments 1, 2 and 3 were 2.53 + 0.24a, 2.73 + 0.36a and 3.53 + 0.28b mm/day; 1.39 + 0.14a, 1.35 + 0.18a dan 1.57 + 0.12b ng/ml/day; and 1.33 + 0.15a, 1.63 + 0.19b and 1.67 + 0.23b mm/day, respectively (P < 0.05). It can be concluded that PGF2 induced corpus luteum regression, decreased in progesterone plasma levels and ovulatory follicular development. Addition of GnRH increased corpus luteum size and plasma progesterone levels, after PGF2 injection corpus luteum regression and progesterone decrease became more prominent, while ovulatory folliculkar development occurred much better. .