bluuebunny/crossref_metadata_2025_split huggingface dataset:
An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation
Authors: Duthoo Evi , Vral Anne , Baeyens Ans 
Published: May 2022

AbstractEven though a detailed understanding of the proliferative characteristics of T lymphocytes is imperative in many research fields, prior studies have never reached a consensus on these characteristics, and on the corresponding cell cycle kinetics specifically. In this study, the general proliferative response of human T lymphocytes to phytohaemagglutinin (PHA) stimulation was characterized using a carboxyfluorescein succinimidyl ester-based flow cytometric assay. We were able to determine when PHA-stimulated T lymphocytes complete their first division, the proportion of cells that initiate proliferation, the subsequent division rate of the cells, and the impact of irradiation on these proliferative properties. Next, we accurately visualized the cell cycle progression of dividing T lymphocytes cultured in whole blood using an adapted 5-ethynyl-2’-deoxyuridine pulse-chase method. Furthermore, through multiple downstream analysis methods, we were able to make an estimation of the corresponding cell cycle kinetics. We also visualized the impact of X-rays on the progression of the cells through the cell cycle. Our results showed dose-dependent G2 arrest after exposure to irradiation, and a corresponding delay in G1 phase-entry of the cells. In conclusion, utilizing various flow cytometric assays, we provided valuable information on T lymphocyte proliferation characteristics starting from first division to fully dividing cells.
Development of a standardized in vitro model to reproduce hydrophilic acrylic intraocular lens calcification
Authors: Britz Leoni , Schickhardt Sonja Katrin , Yildirim Timur Mert , Auffarth Gerd Uwe , Lieberwirth Ingo , Khoramnia Ramin 
Published: May 2022

AbstractOpacification through calcification of hydrophilic acrylic intraocular lenses (IOL) is a severe complication after cataract surgery. Causing symptoms that range from glare through to severe vision loss, the only effective therapy is explantation of the opacified IOL so far. Although IOL calcification is a well-described phenomenon, its pathogenesis is not fully understood yet. The purpose of the current study was to develop a laboratory model to replicate IOL calcification. Calcification could be reproduced using a horizontal electrophoresis and aqueous solutions of calcium chloride and disodium hydrogen phosphate. The analysis of the in vitro calcified IOLs was performed using light microscopy, Alizarin Red and Von Kossa staining, scanning electron microscopy, energy dispersive x-ray spectroscopy and electron crystallography using transmission electron microscopy and electron diffraction. The presented laboratory model could be used to identify hydrophilic IOLs that are at risk to develop calcification and to assess the influence of associated risk factors. In addition, it can serve as a research tool to further understand this pathology.
Incidence and risk factors of progressive nasal inner nuclear layer thickening after surgical peeling of epiretinal membrane
Authors: Park Hyun Young , Yang Jong Yun , Park Hyo Song , Kim Hyesun 
Published: May 2022

AbstractTo assess incidence and risk factors of postoperative progressive nasal inner nuclear layer (INL) thickening after epiretinal membrane (ERM) surgery. Progressive nasal INL thickening was defined as 1.5-fold increase in thickness of nasal INL after ERM surgery compared to preoperative examination. Kaplan–Meier survival analysis was done to compare the cumulative risk ratio between groups stratified by presence of progressive nasal INL thickening. Logistic regression was performed to identify possible risk factors. Progressive nasal INL thickening occurred in 13.0% of ERM removal patients. Patients without progressive nasal INL thickening showed better visual acuity recovery compared to patients with nasal INL thickening (p = 0.029). Presence of cystoid space in inner retinal layer before surgery (odds ratio [OR] = 0.143, 95% confidence interval [CI] 0.028–0.736; p = 0.020), older age (OR = 0.896, 95% CI 0.817–0.982, p = 0.020), and thicker preoperative central macular thickness (OR = 0.994, 95% CI 0.988–1.000, p = 0.039) were correlated inversely with thickening of nasal INL. Correlation between nasal INL thickness and postoperative visual outcome was significant. Absence of cystoid space before ERM surgery, younger age, and thinner central macular thickness were risk factors for progressive postoperative nasal INL thickening. Progressive nasal INL thickening may serve as a new biomarker for worsened visual symptom after ERM surgery.
Gaze direction and face orientation modulate perceptual sensitivity to faces under interocular suppression
Authors: Lanfranco Renzo C. , Stein Timo , Rabagliati Hugh , Carmel David 
Published: May 2022

AbstractFaces convey information essential for social interaction. Their importance has prompted suggestions that some facial features may be processed unconsciously. Although some studies have provided empirical support for this idea, it remains unclear whether these findings were due to perceptual processing or to post-perceptual decisional factors. Evidence for unconscious processing of facial features has predominantly come from the Breaking Continuous Flash Suppression (b-CFS) paradigm, which measures the time it takes different stimuli to overcome interocular suppression. For example, previous studies have found that upright faces are reported faster than inverted faces, and direct-gaze faces are reported faster than averted-gaze faces. However, this procedure suffers from important problems: observers can decide how much information they receive before committing to a report, so their detection responses may be influenced by differences in decision criteria and by stimulus identification. Here, we developed a new procedure that uses predefined exposure durations, enabling independent measurement of perceptual sensitivity and decision criteria. We found higher detection sensitivity to both upright and direct-gaze (compared to inverted and averted-gaze) faces, with no effects on decisional factors. For identification, we found both greater sensitivity and more liberal criteria for upright faces. Our findings demonstrate that face orientation and gaze direction influence perceptual sensitivity, indicating that these facial features may be processed unconsciously.
Quasi-exact solutions for guided modes in two-dimensional materials with tilted Dirac cones
Authors: Ng R. A. , Wild A. , Portnoi M. E. , Hartmann R. R. 
Published: May 2022

AbstractWe show that if the solutions to the (2+1)-dimensional massless Dirac equation for a given one-dimensional (1D) potential are known, then they can be used to obtain the eigenvalues and eigenfunctions for the same potential, orientated at an arbitrary angle, in a 2D Dirac material possessing tilted, anisotropic Dirac cones. This simple set of transformations enables all the exact and quasi-exact solutions associated with 1D quantum wells in graphene to be applied to the confinement problem in tilted Dirac materials such as 8-Pmmn borophene. We also show that smooth electron waveguides in tilted Dirac materials can be used to manipulate the degree of valley polarization of quasiparticles travelling along a particular direction of the channel. We examine the particular case of the hyperbolic secant potential to model realistic top-gated structures for valleytronic applications.
Relationship of isotopic variations with spring density in the structurally controlled springs and related geosystem services in Alaknanda Valley, Garhwal Himalaya, India
Authors: Rawat Aakash Mohan , Bagri Dhirendra Singh , Kumar Sudhir , Badola Ruchi , Hussain Syed Ainul 
Published: May 2022

AbstractAs a traditional water source, springs are vital for Himalayan communities and it is essential to consciously focus on springs conservation. We report oxygen isotopes (δ18O) of spring water before, within, and after the tectonically active zones of the Alaknanda Valley, Uttarakhand. Higher variation of δ18O in the spring waters is found in highly tectonically disturbed zone i.e., Zone-2 with δ18O range − 4.9‰ to − 9.0‰ compared to tectonically less disturbed zones: Zone-1 and Zone-3 with δ18O value range − 7.9‰ to − 9.9‰ and − 7.4 to − 10.2‰ respectively. We hypothesize that the highly active thrust zones (Zone-2) with increased permeability compared to other Zones, manifested as greater spring density, results in higher water recharge in Zone-2. Very high to high spring density stretches are dominant in Zone-2 compared to Zone-1 and Zone-3. Stretches in Zone-2 with high spring density formed due to its highly tectonically active nature leads to the higher isotopic variation in Zone-2. The study also identifies the geosystem services provided by thrust zones as water resources to the local people and need of conservation modalities to manage the spring water resources in the thrust zones.
Virus particle propagation and infectivity along the respiratory tract and a case study for SARS-CoV-2
Authors: Vimalajeewa Dixon , Balasubramaniam Sasitharan , Berry Donagh P. , Barry Gerald 
Published: May 2022

AbstractRespiratory viruses including Respiratory Syncytial Virus, influenza virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cause serious and sometimes fatal disease in thousands of people annually. Understanding virus propagation dynamics within the respiratory system is critical because new insights will increase our understanding of virus pathogenesis and enable infection patterns to be more predictable in vivo, which will enhance our ability to target vaccine and drug delivery. This study presents a computational model of virus propagation within the respiratory tract network. The model includes the generation network branch structure of the respiratory tract, biophysical and infectivity properties of the virus, as well as air flow models that aid the circulation of the virus particles. As a proof of principle, the model was applied to SARS-CoV-2 by integrating data about its replication-cycle, as well as the density of Angiotensin Converting Enzyme expressing cells along the respiratory tract network. Using real-world physiological data associated with factors such as the respiratory rate, the immune response and virus load that is inhaled, the model can improve our understanding of the concentration and spatiotemporal dynamics of the virus. We collected experimental data from a number of studies and integrated them with the model in order to show in silico how the virus load propagates along the respiratory network branches.
High performance visible-SWIR flexible photodetector based on large-area InGaAs/InP PIN structure
Authors: Li Xuanzhang , Zhang Junyang , Yue Chen , Tang Xiansheng , Gao Zhendong , Jiang Yang , Du Chunhua , Deng Zhen , Jia Haiqiang , Wang Wenxin , Chen Hong 
Published: May 2022

AbstractMechanically flexible optoelectronic devices and systems can enable a much broader range of applications than what their rigid counterparts can do, especially for novel bio-integrated optoelectronic systems, flexible consumer electronics and wearable sensors. Inorganic semiconductor could be a good candidate for the flexible PD when it can keep its high performance under the bending condition. Here, we demonstrate a III–V material-based flexible photodetector operating wavelength from 640 to 1700 nm with the high detectivity of 5.18 × 1011 cm‧Hz1/2/W and fast response speed @1550 nm by using a simply top-to-down fabrication process. The optoelectrical performances are stable as the PDs are exposed to bending cycles with a radius of 15 mm up to 1000 times. Furthermore, the mechanical failure mode of the PD is also investigated, which suggests that the cracking and delamination failure mode are dominant in bending up and bending down direction, respectively. Such a flexible III–V material-based PD and design with stable and high performance could be a promising strategy for the application of the flexible broad spectrum detection.
Nuclear soluble cGAS senses double-stranded DNA virus infection
Authors: Wu Yakun , Song Kun , Hao Wenzhuo , Li Jack , Wang Lingyan , Li Shitao 
Published: May 2022

AbstractThe DNA sensor cGAS detects cytosolic DNA and instigates type I interferon (IFN) expression. Recent studies find that cGAS also localizes in the nucleus and binds the chromatin. Despite the mechanism controlling nuclear cGAS activation is well elucidated, whether nuclear cGAS participates in DNA sensing is unclear. Here, we report that herpes simplex virus 1 (HSV-1) infection caused the release of cGAS from the chromatin into the nuclear soluble fraction. Like its cytosolic counterpart, the leaked nuclear soluble cGAS also sensed viral DNA, produced cGAMP, and induced mRNA expression of type I IFN and interferon-stimulated genes. Consistently, the nuclear soluble cGAS limited HSV-1 infection. Furthermore, enzyme-deficient mutation (D307A) or cGAS inhibitor RU.251 abolished nuclear cGAS-mediated innate immune responses, suggesting that enzymatic activity is also required for nuclear soluble cGAS. Taken all together, our study demonstrates that nuclear soluble cGAS acts as a nuclear DNA sensor detecting nuclear-replicating DNA viruses.
Clinical perspectives on vagus nerve stimulation: present and future
Authors: Goggins Eibhlin , Mitani Shuhei , Tanaka Shinji 
Published: May 2022

Abstract
The vagus nerve, the great wanderer, is involved in numerous processes throughout the body and vagus nerve stimulation (VNS) has the potential to modulate many of these functions. This wide-reaching capability has generated much interest across a range of disciplines resulting in several clinical trials and studies into the mechanistic basis of VNS. This review discusses current preclinical and clinical evidence supporting the efficacy of VNS in different diseases and highlights recent advancements. Studies that provide insights into the mechanism of VNS are considered.
Influence of protostellar jets and HII regions on the formation and evolution of stellar clusters
Authors: Verliat Antoine , Hennebelle Patrick , González Marta , Lee Yueh-Ning , Geen Sam 
Published: May 2022

Context.Understanding the conditions in which stars and stellar clusters form is of great importance. In particular, the role that stellar feedback may have is still hampered by large uncertainties.Aims.We aim to investigate the role played by ionising radiation and protostellar outflows during the formation and evolution of a stellar cluster. To self-consistently take into account gas accretion, we start with clumps of tens of parsecs in size.Methods.Using an adaptive mesh refinement code, we ran magneto-hydrodynamical numerical simulations aimed at describing the collapse of massive clumps with either no stellar feedback or taking into account ionising radiation and/or protostellar jets.Results.Stellar feedback substantially modifies the protostellar cluster properties in several ways. We confirm that protostellar outflows reduce the star formation rate by a factor of a few, although the outflows do not stop accretion and, likely enough, do not modify the final cluster mass. On the other hand, once sufficiently massive stars have formed, ionising radiation efficiently expels the remaining gas and reduces the final cluster mass by a factor of several. We found that while HII radiation and jets barely change the distribution of high density gas, the latter increases the dense gas velocity dispersion again by a factor of several in a few places. As we are starting from a relatively large scale, we found that the clusters whose mass and size are, respectively, of the order of a few 1000M⊙and a fraction of parsec, present a significant level of rotation. Moreover, we found that the sink particles that mimic the stars themselves tend to have rotation axes aligned with the cluster’s large-scale rotation. Finally, computing the classicalQparameter used to quantify stellar cluster structure, we infer that when jets are included in the calculation, theQvalues are typical of observations, while when protostellar jets are not included, theQvalues tend to be significantly lower. This is due to the presence of sub-clustering that is considerably reduced by the jets.Conclusions.Both large-scale gas accretion and stellar feedback, namely HII regions and protostellar jets, appear to significantly influence the formation and evolution of stellar clusters.
Polarization power spectra and dust cloud morphology
Authors: Konstantinou A. , Pelgrims V. , Fuchs F. , Tassis K. 
Published: May 2022

Context In the framework of studying cosmic microwave background polarization and characterizing its Galactic foregrounds, the angular power spectrum analysis of the thermal dust polarization map has led to intriguing evidence of an E/B asymmetry and a positive TE correlation. The interpretation of these observations is the subject of theoretical and simulation-driven studies in which the correlation between the density structure of the interstellar medium (ISM) and the magnetic field appears to be a key aspect. In this context, and when the magnetized ISM structures are modeled in three dimensions, dust clouds are generally considered to be filamentary structures only, but both filamentary and sheet-like shapes are supported by observational and theoretical evidence.
Aims. We aim to study the influence of the cloud shape and its connection to the local magnetic field, as well as the influence from the viewing angle, on the angular power spectra measured on thermal dust polarization maps; we specifically focus on the dependence of the E/B power asymmetry and TE correlation.
Methods. To this end, we simulated realistic interstellar clouds with both filament-like and sheet-like shapes using the software Asterion, which also allowed us to generate synthetic maps of thermal dust polarized emission with an area of 400 square degrees. Then, we computed their polarization power spectra in the multipole range ℓ ϵ [100, 500] and focused on the E/B power asymmetry, quantified through the ℛEB ratio, and the correlation coefficient rTE between Τ and Ε modes. We quantified the dependence of ℛEB and rTE values on the offset angle (between the longest cloud axis and local magnetic field lines) and inclination angle (between the line of sight and the magnetic field) for both types of cloud shapes, either embedded in a regular magnetic field or coupled to a nonregular field to mimic turbulence.
Results. We find that both types of cloud shapes cover the same regions of the (ℛEB, rTE) parameter space. The dependence on the inclination and offset angles is similar for both shapes, although sheet-like structures generally show larger scatter than filamentary structures. In addition to the known dependence on the offset angle, we find a strong dependence of ℛEB and rTE on the inclination angle.
Conclusions. The very fact that filament-like and sheet-like structures may lead to polarization power spectra with similar (ℛEB,rTE) values complicates their interpretation. We argue that interpreting them solely in terms of filament characteristics is risky, and in future analyses, this degeneracy should be accounted for, as should the connection to the magnetic field geometry. Our results based on maps of 400 square degrees clarify that the overall geometrical arrangement of the magnetized ISM surrounding the observer leaves its marks on polarization power spectra.
The eccentric millisecond pulsar, PSR J0955−6150
Authors: Serylak M. , Venkatraman Krishnan V. , Freire P. C. C. , Tauris T. M. , Kramer M. , Geyer M. , Parthasarathy A. , Bailes M. , Bernadich M. C. i , Buchner S. , Burgay M. , Camilo F. , Karastergiou A. , Lower M. E. , Possenti A. , Reardon D. J. , Shannon R. M. , Spiewak R. , Stairs I. H. , van Straten W. 
Published: May 2022

Context.PSR J0955−6150 is a member of an enigmatic class of eccentric millisecond pulsar (MSP) and helium white dwarf (He WD) systems (eMSPs), whose binary evolution is poorly understood and believed to be strikingly different to that of traditional MSP+He WD systems in circular orbits.Aims.Measuring the masses of the stars in this system is important for testing the different hypotheses for the formation of eMSPs.Methods.We carried out timing observations of this pulsar with the Parkes radio telescope using the 20 cm multibeam and ultra-wide bandwidth low-frequency (UWL) receivers, and theL-band receiver of the MeerKAT radio telescope. The pulse profiles were flux and polarisation calibrated, and a rotating-vector model (RVM) was fitted to the position angle of the linear polarisation of the combined MeerKAT data. Pulse times of arrival (ToAs) were obtained from these using standard pulsar analysis techniques and analysed using theTEMPO2 timing software.Results.Our observations reveal a strong frequency evolution of this MSP’s intensity, with a flux density spectral index (α) of −3.13(2). The improved sensitivity of MeerKAT resulted in a greater than tenfold improvement in the timing precision obtained compared to our older Parkes observations. This, combined with the eight-year timing baseline, has allowed precise measurements of a very small proper motion and three orbital post-Keplerian parameters, namely the rate of advance of periastron,ω̇ = 0.00152(1) deg yr−1, and the orthometric Shapiro delay parameters,h3 = 0.89(7) μs and ς = 0.88(2). Assuming general relativity, we obtainMp = 1.71(2) M⊙for the mass of the pulsar andMc = 0.254(2) M⊙for the mass of the companion; the orbital inclination is 83.2(4) degrees. Crucially, assuming that the position angle of the linear polarisation follows the RVM, we find that the spin axis has a misalignment relative to the orbital angular momentum of > 4.8deg at 99% confidence level.Conclusions.While the value ofMpfalls well within the wide range observed in eMSPs,Mcis significantly smaller than expected from several formation hypotheses proposed, which are therefore unlikely to be correct and can be ruled out;Mcis also significantly different from the expected value for an ideal low mass X-ray binary evolution scenario. If the misalignment between the spin axis of the pulsar and the orbital angular momentum is to be believed, it suggests that the unknown process that created the orbital eccentricity of the binary was also capable of changing its orbital orientation, an important evidence for understanding the origin of eMSPs.