bluuebunny/crossref_metadata_2025_split huggingface dataset:
Annexin A6 and NPC1 regulate LDL-inducible cell migration and distribution of focal adhesions
Authors: Jose Jaimy , Hoque Monira , Engel Johanna , Beevi Syed S. , Wahba Mohamed , Georgieva Mariya Ilieva , Murphy Kendelle J. , Hughes William E. , Cochran Blake J. , Lu Albert , Tebar Francesc , Hoy Andrew J. , Timpson Paul , Rye Kerry-Anne , Enrich Carlos , Rentero Carles , Grewal Thomas 
Published: January 2022

AbstractCholesterol is considered indispensable for cell motility, but how physiological cholesterol pools enable cells to move forward remains to be clarified. The majority of cells obtain cholesterol from the uptake of Low-Density lipoproteins (LDL) and here we demonstrate that LDL stimulates A431 squamous epithelial carcinoma and Chinese hamster ovary (CHO) cell migration and invasion. LDL also potentiated epidermal growth factor (EGF) -stimulated A431 cell migration as well as A431 invasion in 3-dimensional environments, using organotypic assays. Blocking cholesterol export from late endosomes (LE), using Niemann Pick Type C1 (NPC1) mutant cells, pharmacological NPC1 inhibition or overexpression of the annexin A6 (AnxA6) scaffold protein, compromised LDL-inducible migration and invasion. Nevertheless, NPC1 mutant cells established focal adhesions (FA) that contain activated focal adhesion kinase (pY397FAK, pY861FAK), vinculin and paxillin. Compared to controls, NPC1 mutants display increased FA numbers throughout the cell body, but lack LDL-inducible FA formation at cell edges. Strikingly, AnxA6 depletion in NPC1 mutant cells, which restores late endosomal cholesterol export in these cells, increases their cell motility and association of the cholesterol biosensor D4H with active FAK at cell edges, indicating that AnxA6-regulated transport routes contribute to cholesterol delivery to FA structures, thereby improving NPC1 mutant cell migratory behaviour.
Machine learning algorithms as new screening approach for patients with endometriosis
Authors: Bendifallah Sofiane , Puchar Anne , Suisse Stéphane , Delbos Léa , Poilblanc Mathieu , Descamps Philippe , Golfier Francois , Touboul Cyril , Dabi Yohann , Daraï Emile 
Published: January 2022

AbstractEndometriosis—a systemic and chronic condition occurring in women of childbearing age—is a highly enigmatic disease with unresolved questions. While multiple biomarkers, genomic analysis, questionnaires, and imaging techniques have been advocated as screening and triage tests for endometriosis to replace diagnostic laparoscopy, none have been implemented routinely in clinical practice. We investigated the use of machine learning algorithms (MLA) in the diagnosis and screening of endometriosis based on 16 key clinical and patient-based symptom features. The sensitivity, specificity, F1-score and AUCs of the MLA to diagnose endometriosis in the training and validation sets varied from 0.82 to 1, 0–0.8, 0–0.88, 0.5–0.89, and from 0.91 to 0.95, 0.66–0.92, 0.77–0.92, respectively. Our data suggest that MLA could be a promising screening test for general practitioners, gynecologists, and other front-line health care providers. Introducing MLA in this setting represents a paradigm change in clinical practice as it could replace diagnostic laparoscopy. Furthermore, this patient-based screening tool empowers patients with endometriosis to self-identify potential symptoms and initiate dialogue with physicians about diagnosis and treatment, and hence contribute to shared decision making.
Intraoperative hyperspectral imaging (HSI) as a new diagnostic tool for the detection of cartilage degeneration
Authors: Kistler Max , Köhler Hannes , Theopold Jan , Gockel Ines , Roth Andreas , Hepp Pierre , Osterhoff Georg 
Published: January 2022

AbstractTo investigate, whether hyperspectral imaging (HSI) is able to reliably differentiate between healthy and damaged cartilage tissue. A prospective diagnostic study was performed including 21 patients undergoing open knee surgery. HSI data were acquired during surgery, and the joint surface’s cartilage was assessed according to the ICRS cartilage injury score. The HSI system records light spectra from 500 to 1000 nm and generates several parameters including tissue water index (TWI) and the absorbance at 960 nm and 540 nm. Receiver operating characteristic curves were calculated to assess test parameters for threshold values of HSI. Areas with a cartilage defect ICRS grade ≥ 3 showed a significantly lower TWI (p = 0.026) and higher values for 540 nm (p < 0.001). No difference was seen for 960 nm (p = 0.244). For a threshold of 540 nm > 0.74, a cartilage defect ICRS grade ≥ 3 could be detected with a sensitivity of 0.81 and a specificity of 0.81. TWI was not suitable for cartilage defect detection. HSI can provide reliable parameters to differentiate healthy and damaged cartilage. Our data clearly suggest that the difference in absorbance at 540 nm would be the best parameter to achieve accurate identification of damaged cartilage.
Combining higher accumulation of amylopectin, lysine and tryptophan in maize hybrids through genomics-assisted stacking of waxy1 and opaque2 genes
Authors: Talukder Zahirul A. , Muthusamy Vignesh , Chhabra Rashmi , Gain Nisrita , Reddappa Shashidhar B. , Mishra Subhra J. , Kasana Ravindra , Bhatt Vinay , Chand Gulab , Katral Ashvinkumar , Mehta Brijesh K. , Guleria Satish K. , Zunjare Rajkumar U. , Hossain Firoz 
Published: January 2022

AbstractWaxy maize rich in amylopectin has emerged as a preferred food. However, waxy maize is poor in lysine and tryptophan, deficiency of which cause severe health problems. So far, no waxy hybrid with high lysine and tryptophan has been developed and commercialized. Here, we combined recessive waxy1 (wx1) and opaque2 (o2) genes in the parental lines of four popular hybrids (HQPM1, HQPM4, HQPM5, and HQPM7) using genomics-assisted breeding. The gene-based markers, wx-2507F/RG and phi057 specific for wx1 and o2, respectively were successfully used to genotype BC1F1, BC2F1 and BC2F2 populations. Background selection with > 100 SSRs resulted in recovering > 94% of the recurrent parent genome. The reconstituted hybrids showed 1.4-fold increase in amylopectin (mean: 98.84%) compared to the original hybrids (mean: 72.45%). The reconstituted hybrids also showed 14.3% and 14.6% increase in lysine (mean: 0.384%) and tryptophan (mean: 0.102%), respectively over the original hybrids (lysine: 0.336%, tryptophan: 0.089%). Reconstituted hybrids also possessed similar grain yield (mean: 6248 kg/ha) with their original versions (mean: 6111 kg/ha). The waxy hybrids with high lysine and tryptophan assume great significance in alleviating malnutrition through sustainable and cost-effective means. This is the first report of development of lysine and tryptophan rich waxy hybrids using genomics-assisted selection.
High glycemic albumin representing prestroke glycemic variability is associated with hemorrhagic transformation in patients receiving intravenous thrombolysis
Authors: Lee Sang-Hwa , Jang Min Uk , Kim Yerim , Park So Young , Kim Chulho , Kim Yeo Jin , Sohn Jong-Hee 
Published: January 2022

AbstractWe evaluated the impact of prestroke glycemic variability estimated by glycated albumin (GA) on symptomatic hemorrhagic transformation (SHT) in patients with intravenous thrombolysis (IVT). Using a multicenter database, we consecutively enrolled acute ischemic stroke patients receiving IVT. A total of 378 patients were included in this study. Higher GA was defined as GA ≥ 16.0%. The primary outcome measure was SHT. Multivariate regression analysis and a receiver operating characteristic curve were used to assess risks and predictive ability for SHT. Among the 378 patients who were enrolled in this study, 27 patients (7.1%) had SHT as defined by the Safe Implementation of Thrombolysis in Stroke-Monitoring Study (SHTSITS). The rate of SHTSITS was higher in the higher GA group than in the lower GA group (18.0% vs. 1.6%, p < 0.001). A higher GA level (GA ≥ 16.0%) significantly increased the risk of SHTSITS (adjusted odds ratio [OR], [95% confidence interval, CI], 12.57 [3.08–41.54]) in the logistic regression analysis. The predictive ability of the GA level for SHTSITS was good (AUC [95% CI]: 0.83 [0.77–0.90], p < 0.001), and the cutoff value of GA in SHT was 16.3%. GA was a reliable predictor of SHT after IVT in acute ischemic stroke in this study.
Increased occurrence of high impact compound events under climate change
Authors: Ridder N. N. , Ukkola A. M. , Pitman A. J. , Perkins-Kirkpatrick S. E. 
Published: January 2022

AbstractWhile compound weather and climate events (CEs) can lead to significant socioeconomic consequences, their response to climate change is mostly unexplored. We report the first multi-model assessment of future changes in return periods for the co-occurrence of heatwaves and drought, and extreme winds and precipitation based on the Coupled Model Intercomparison Project (CMIP6) and three emission scenarios. Extreme winds and precipitation CEs occur more frequently in many regions, particularly under higher emissions. Heatwaves and drought occur more frequently everywhere under all emission scenarios examined. For each CMIP6 model, we derive a skill score for simulating CEs. Models with higher skill in simulating historical CEs project smaller increases in the number of heatwaves and drought in Eurasia, but larger numbers of strong winds and heavy precipitation CEs everywhere for all emission scenarios. This result is partly masked if the whole CMIP6 ensemble is used, pointing to the considerable value in further improvements in climate models.
Zero-field skyrmionic states and in-field edge-skyrmions induced by boundary tuning
Authors: Spethmann Jonas , Vedmedenko Elena Y. , Wiesendanger Roland , Kubetzka André , von Bergmann Kirsten 
Published: January 2022

AbstractWhen magnetic skyrmions are moved via currents, they do not strictly travel along the path of the current, instead their motion also gains a transverse component. This so-called skyrmion Hall effect can be detrimental in potential skyrmion devices because it drives skyrmions towards the edge of their hosting material where they face potential annihilation. Here we experimentally modify a skyrmion model system—an atomic Pd/Fe bilayer on Ir(111)—by decorating the film edge with ferromagnetic Co/Fe patches. Employing spin-polarized scanning tunneling microscopy, we demonstrate that this ferromagnetic rim prevents skyrmion annihilation at the film edge and stabilizes skyrmions and target states in zero field. Furthermore, in an external magnetic field the Co/Fe rim can give rise to skyrmions pinned to the film edge. Spin dynamics simulations reveal how a combination of different attractive and repulsive skyrmion-edge interactions can induce such an edge-pinning effect for skyrmions.
Tracking the time evolution of soft matter systems via topological structural heterogeneity
Authors: Membrillo Solis Ingrid , Orlova Tetiana , Bednarska Karolina , Lesiak Piotr , Woliński Tomasz R. , D’Alessandro Giampaolo , Brodzki Jacek , Kaczmarek Malgosia 
Published: January 2022

AbstractPersistent homology is an effective topological data analysis tool to quantify the structural and morphological features of soft materials, but so far it has not been used to characterise the dynamical behaviour of complex soft matter systems. Here, we introduce structural heterogeneity, a topological characteristic for semi-ordered materials that captures their degree of organisation at a mesoscopic level and tracks their time-evolution, ultimately detecting the order-disorder transition at the microscopic scale. We show that structural heterogeneity tracks structural changes in a liquid crystal nanocomposite, reveals the effect of confined geometry on the nematic-isotropic and isotropic-nematic phase transitions, and uncovers physical differences between these two processes. The system used in this work is representative of a class of composite nanomaterials, partially ordered and with complex structural and physical behaviour, where their precise characterisation poses significant challenges. Our developed analytic framework can provide both a qualitative and quantitative characterisation of the dynamical behaviour of a wide range of semi-ordered soft matter systems.
Clinical validation of engineered CRISPR/Cas12a for rapid SARS-CoV-2 detection
Authors: Nguyen Long T. , Rananaware Santosh R. , Pizzano Brianna L. M. , Stone Brandon T. , Jain Piyush K. 
Published: January 2022

Abstract
Background
The coronavirus disease (COVID-19) caused by SARS-CoV-2 has swept through the globe at an unprecedented rate. CRISPR-based detection technologies have emerged as a rapid and affordable platform that can shape the future of diagnostics.

Methods
We developed ENHANCEv2 that is composed of a chimeric guide RNA, a modified LbCas12a enzyme, and a dual reporter construct to improve the previously reported ENHANCE system. We validated both ENHANCE and ENHANCEv2 using 62 nasopharyngeal swabs and compared the results to RT-qPCR. We created a lyophilized version of ENHANCEv2 and characterized its detection capability and stability.

Results
Here we demonstrate that when coupled with an RT-LAMP step, ENHANCE detects COVID-19 samples down to a few copies with 95% accuracy while maintaining a high specificity towards various isolates of SARS-CoV-2 against 31 highly similar and common respiratory pathogens. ENHANCE works robustly in a wide range of magnesium concentrations (3 mM-13 mM), allowing for further assay optimization. Our clinical validation results for both ENHANCE and ENHANCEv2 show 60/62 (96.7%) sample agreement with RT-qPCR results while only using 5 µL of sample and 20 minutes of CRISPR reaction. We show that the lateral flow assay using paper-based strips displays 100% agreement with the fluorescence-based reporter assay during clinical validation. Finally, we demonstrate that a lyophilized version of ENHANCEv2 shows high sensitivity and specificity for SARS-CoV-2 detection while reducing the CRISPR reaction time to as low as 3 minutes while maintaining its detection capability for several weeks upon storage at room temperature.

Conclusions
CRISPR-based diagnostic platforms offer many advantages as compared to conventional qPCR-based detection methods. Our work here provides clinical validation of ENHANCE and its improved form ENHANCEv2 for the detection of COVID-19.
Cross-Language Transfer and Attentional Control in Early Bilingual Speech
Authors: Verbeek Lisa , Vissers Constance , Blumenthal Mirjam , Verhoeven Ludo 
Published: January 2022

Purpose:This study investigated the roles of cross-language transfer of first language (L1) and attentional control in second-language (L2) speech perception and production of sequential bilinguals, taking phonological overlap into account.Method:Twenty-five monolingual Dutch-speaking and 25 sequential bilingual Turkish–Dutch-speaking 3- and 4-year-olds were tested using picture identification tasks for speech perception in L1 Turkish and L2 Dutch, single-word tasks for speech production in L1 and L2, and a visual search task for attentional control. Phonological overlap was manipulated by dividing the speech tasks into subsets of phonemes that were either shared or unshared between languages.Results:In Dutch speech perception and production, monolingual children obtained higher accuracies than bilingual peers. Bilinguals showed equal performance in L1 and L2 perception but scored higher on L1 than on L2 production. For speech perception of shared phonemes, linear regression analyses revealed no direct effects of attention and L1 on L2. For speech production of shared phonemes, attention and L1 directly affected L2. When exploring unshared phonemes, direct effects of attentional control on L2 were demonstrated not only for speech production but also for speech perception.Conclusions:The roles of attentional control and cross-language transfer on L2 speech are different for shared and unshared phonemes. Whereas L2 speech production of shared phonemes is also supported by cross-language transfer of L1, L2 speech perception and production of unshared phonemes benefit from attentional control only. This underscores the clinical importance of considering phonological overlap and supporting attentional control when assisting young sequential bilinguals' L2 development.
Tele-Ethics in Communication Disorders: Asking the Right Questions, Finding the Right Answers
Authors: Hall Kellyn Dailey 
Published: January 2022

Purpose:
A hypothetical case is used to illustrate legal and ethical issues involving the decision to replace the traditional in-person service delivery model with telepractice in schools beyond the context of the initial COVID-19 health emergency. In this clinical focus article, the reader follows Maria, the lead speech-language pathologist (SLP) in the district, as she determines the feasibility of continuing telepractice in her district now that students and clinicians are returning to schools. First, she considers the support needed to implement this service delivery model within the school setting given the anticipated changes to the rules and regulations governing lawful and ethical provision of telepractice after the health emergency ends. Next, she decides if telepractice is warranted in the district by considering the rationales behind the requests. Faced with balancing school, student, and clinician needs, Maria uses an ethical decision-making model to determine if requests for telepractice, tied to health safety concerns and potentially influenced by implicit bias, reflect legal, ethical, and/or moral issues driven by fear or unconscious discriminatory motives.

Conclusions:
The health emergency gave SLPs working in schools the unique opportunity to experience the benefits and utility of telepractice. Following the return to schools, continuation of telepractice services will require support and training of SLPs. Many factors must be considered including equivalency of services, technology, and protection of privacy as they relate to the changes to the laws and regulations governing telepractice after the health emergency allowances end. Of primary importance is the selection of telepractice to address student needs, not to avoid specific schools because of their characteristics or location. An ethical decision-making model can be used as a framework to guide service delivery model decisions that balance the needs of the student, the clinician, and the district.
MIMO antenna for IOT devices with reconfigurable radiation pattern
Authors: Shin Hyunwoong , Kim Hyeongdong 
Published: January 2022

AbstractHere, how to control isolation and correlation by adjusting the position of capacitors in a resonance structure in a MIMO ground radiation antenna is studied. The MIMO system consisting of two 7 mm × 7 mm printed ground radiation antennas operating at WLAN on a 40 mm × 40 mm ground plane was installed. If the positions of the capacitors of the two antennas are equally on the top of the ground plane, S12 is −5.2 dB and envelope correlation coefficient is 0.47. When the capacitors are orthogonal to each other, S12 is improved to −16.5 dB and envelope correlation coefficient is improved to 0.12. The orthogonality of the characteristic mode of the ground plane by the antennas is confirmed by the radiation patterns when the position of the capacitor is orthogonal.
Heuristic search for multi‐distance measuring equipment station selection
Authors: Liang Xiao , Milner Carl , Macabiau Christophe , Estival Philippe 
Published: January 2022

AbstractTo support alternative positioning, navigation and timing (A‐PNT), multi‐DME with better geometry than distance measuring equipment (DME)/DME can be used to improve the performance. Different from the existing station selection methods that have been proposed for DME/DME, a station selection method for multi‐DME should be considered in order to prevent unnecessary emission of navigation signals because DME receivers have limited number of channels and constrained real‐time processing power, and also to limit DME to GNSS L5/E5a interference. A heuristic algorithm is constructed to determine the quasi‐optimal selection of DME stations based on geometrical and computational cost restrictions, which is different from a brute‐force method checking all possible subsets and selecting the best one. A station selection method for multi‐DME is proposed based on the heuristic search. Meanwhile, the degradation of GNSS L5/E5a signal processing that is caused by DME signals is discussed. An advanced station selection method where the DME to GNSS signal interference is taken into account is also proposed. The experimental results show that the methods based on heuristic search can effectively select the quasi‐optimal subset of DME stations with less computational expense.
Lichttherapie zur Delirprävention bei Intensivpatienten: Was sagt die Evidenz?
Authors: Schmidt Sebastian , Hancke Laura , Spies Claudia , Piazena Helmut , Luetz Alawi 
Published: January 2022

ZusammenfassungDie zirkadiane Dysrhythmie betrifft die Mehrzahl der Intensivpatienten und hat weitreichende Auswirkungen auf Organfunktionen. Auf der Ebene des zentralen Nervensystems werden kognitive Exekutivfunktionen beeinträchtigt und die Entwicklung eines Delirs gefördert. Aus diesem Grund sollten mehrdimensionale, patientenindividualisierte chronotherapeutische Konzepte für die Intensivmedizin entwickelt werden. Die zirkadiane Lichttherapie ist in diesem Kontext ein vielversprechender Ansatz.
Konzepte zur Reduktion der Lärmbelastung auf der Intensivstation
Authors: Theuerkauf Nils-Ulrich , Putensen Christian , Schewe Jens-Christian 
Published: January 2022

ZusammenfassungDie Lärmbelastung auf der Intensivstation ist ein allgegenwärtiges, erhebliches Problem für Patienten und Personal. Viele maßgebliche Lärmquellen sind mitverantwortlich für die häufigen Schlafstörungen der Patienten, die das Auftreten eines Delirs begünstigen. Der Beitrag fasst die aktuelle Evidenz zur Entstehung lärmassoziierter Auswirkungen zusammen und zeigt Optionen zur Lärmreduktion und Verbesserung der Therapie von Intensivpatienten auf.
Sulpirid induzierte Dystonie bei einem Patienten mit Tourette-Syndrom
- eine Schlaganfall ähnliche Präsentation
Authors: Abdelnaby Ramy , Abosrea Mona Ali , Schönfeldt-Lecuona Carlos , Rudek Markus A. , Elsayed Mohamed 
Published: January 2022

ZusammenfassungWir berichten über den Fall einer Sulpirid-induzierten Dystonie bei einem
Patienten mit einem bekannten Tourette Syndrom. Aufgrund des plötzlichen
Auftretens einer schlaganfallähnlichen Symptomatik während einer
körperlichen Belastung war die eigentliche Dystoniediagnose erschwert.
Nach Ausschluss einer vaskulären Ursache (Blutung,
Gefäßdissektion, etc.) durch geeigneter Bildgebung wurde
Biperiden infundiert, welche zum sofortigen Verschwinden der Dystonie
führte.