bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kinetics of microbial and photochemical degradation of aflatoxin B1 in a sandy loam and clay soil
Authors: Albert Julius , Muñoz Katherine 
Published: October 2022

AbstractIn a 28-days experiment, we investigated the dissipation of aflatoxin B1 (AFB1) (0.5–500 $${\upmu }\text {g}\,\text {kg}^{-1}$$

μ
g

kg

-
1

) by microbial (MD) and photodegradation (PD) in two contrasting soils (sandy loam and clay). Sterile incubation in darkness served as control (C). AFB1 was degraded in all scenarios according to simple first-order kinetics with 50% dissipation times of 20–32 (PD), 19–48 (MD), and 56–65 days (C), respectively. Dissipation rates were significantly lower ($$\text {p}<0.001$$

p
<
0.001

) in the clay soil than in the sandy loam soil, likely due to photoquenching and strong binding of AFB1 by clay minerals and humic substances. In the sandy loam, dissipation rate of MD decreased in function of initial AFB1 concentration, probably due to toxic effects on degrading microbes. In contrast, in the clay soil the dissipation rate increased with increasing concentration up to 250 $$\upmu \text {g}\,\text {kg}^{-1}$$

μ
g

kg

-
1

, followed by a sharp decrease at 500 $$\upmu \text {g}\,\text {kg}^{-1}$$

μ
g

kg

-
1

, indicating an effect of soil texture on the bioavailability of AFB1 to soil microbes. AFB2a was identified as a transformation product in all scenarios. These results confirm the function of soil for AFB1 degradation, which is modulated by abiotic and biotic processes, soil characteristics and initial AFB1 concentration.
Unsupervised recognition of components from the interaction of BSA with Fe cluster in different conditions utilizing 2D fluorescence spectroscopy
Authors: Kompany-Zareh Mohsen , Akbarian Somayyeh , Najafpour Mohammad Mahdi 
Published: October 2022

AbstractThe excitation-emission fluorescence spectroscopy combined with three-way analysis was applied for discriminating the pure BSA and BSA/Fe3O(OAc)6ClO4 (Fe) using unsupervised classification methods. Herein, the interaction of bovine serum albumin (BSA) and Fe clusters as an artificial enzyme is studied by extracting the intrinsic excitation-emission (EEM) fluorescence of BSA. The conformation of BSA changes with pH, temperature, and Fe concentration. Three-way fluorescence data were recorded for BSA and BSA/Fe during different days. The obtained results showed that the Fe clusters cause changes in the structure of BSA conformation as a function of pH, temperature, and Fe concentration. Also, the denaturation pathway of the BSA molecule is significantly different in the presence of Fe clusters. Both techniques of PARAFAC and PCA were used in the excitation-emission fluorescence matrices (EEM) of solutions at three different pH (5.0, 7.0, and 9.0) and temperatures (15.0, 25.0, and 35.0 °C) values. Also, we reported the results of the change in concentrations of Fe (4.0, 6.0, and 8.0 mg) using these methods. These three amino acids (tyrosine, tryptophan, and phenylalanine) indicate all datasets and their similarities and differences. The spectral differences were more remarkable in different pH values compared to different temperatures. Also, we could distinguish between the groups of protein samples properly in different concentrations of Fe using low-cost EEM spectral images and PARAFAC.
Spontaneous activity patterns in human motor cortex replay evoked activity patterns for hand movements
Authors: Livne Tomer , Kim DoHyun , Metcalf Nicholas V. , Zhang Lu , Pini Lorenzo , Shulman Gordon L. , Corbetta Maurizio 
Published: October 2022

AbstractSpontaneous brain activity, measured with resting state fMRI (R-fMRI), is correlated among regions that are co-activated by behavioral tasks. It is unclear, however, whether spatial patterns of spontaneous activity within a cortical region correspond to spatial patterns of activity evoked by specific stimuli, actions, or mental states. The current study investigated the hypothesis that spontaneous activity in motor cortex represents motor patterns commonly occurring in daily life. To test this hypothesis 15 healthy participants were scanned while performing four different hand movements. Three movements (Grip, Extend, Pinch) were ecological involving grip and grasp hand movements; one control movement involving the rotation of the wrist was not ecological and infrequent (Shake). They were also scanned at rest before and after the execution of the motor tasks (resting-state scans). Using the task data, we identified movement-specific patterns in the primary motor cortex. These task-defined patterns were compared to resting-state patterns in the same motor region. We also performed a control analysis within the primary visual cortex. We found that spontaneous activity patterns in the primary motor cortex were more like task patterns for ecological than control movements. In contrast, there was no difference between ecological and control hand movements in the primary visual area. These findings provide evidence that spontaneous activity in human motor cortex forms fine-scale, patterned representations associated with behaviors that frequently occur in daily life.
Flood impacts on urban road connectivity in southern China
Authors: Zhou Ruitao , Zheng Hang , Liu Yueyi , Xie Guanti , Wan Wenhua 
Published: October 2022

AbstractEffective measures to improve road accessibility during storms are required as traffic congestion caused by storm floods increasingly constrains the efficiency of urban commuting. However, flood impacts on urban road connectivity are not yet well assessed due to inaccurate simulation of flood processes in urban areas where high-resolution data for drainage networks and gauged hydrological data are insufficient. Thus, this study assesses flood impacts on road network connectivity in an urban area of southern China through joint modeling of 1-D hydrodynamic processes in drainage networks and 2-D flood inundation processes on roads using MIKE Urban and MIKE 21. High-resolution DEM images of 5 m and a drainage network of 5635 pipelines were used for urban hydrological simulation. Flood depths were gauged for model calibration and validation by recruited volunteers in the context of citizen science. The results show that road network connectivity decreases as rainfall increases. More than 40% of road connectivity is lost in the study area when a 1-in-100-year return period rainfall occurs. The study results can help to inform more adaptive strategies for local flood control. The study methods are also applicable to improving urban hydrological modeling in broader regions.
Applying genomic approaches to delineate conservation strategies using the freshwater mussel Margaritifera margaritifera in the Iberian Peninsula as a model
Authors: Perea S. , Mendes S. L. , Sousa-Santos C. , Ondina P. , Amaro R. , Castro J. , San-Miguel E. , Lima C. S. , Garcia M. , Velasquez V. , Garcia-Roves P. , Fernández D. , Araujo R. , Sousa V. C. , Reis J. 
Published: October 2022

AbstractEffective conservation actions to counteract the current decline of populations and species require a deep knowledge on their genetic structure. We used Single Nucleotide Polymorphisms (SNPs) to infer the population structure of the highly threatened freshwater pearl mussel Margaritifera margaritifera in the Iberian Peninsula. A total of 130 individuals were collected from 26 locations belonging to 16 basins. We obtained 31,692 SNPs through Genotyping by Sequencing (GBS) and used this dataset to infer population structure. Genetic diversity given as observed heterozygosity was low. Pairwise FST comparisons revealed low levels of genetic differentiation among geographically close populations. Up to 3 major genetic lineages were determined: Atlantic, Cantabrian and Douro. This structure suggests a close co-evolutionary process with brown trout (Salmo trutta), the primordial fish host of this mussel in the studied area. Some sub-basins showed some genetic structuring, whereas in others no intrapopulation differentiation was found. Our results confirm that genetic conservation units do not match individual basins, and that knowledge about the genetic structure is necessary before planning recovery plans that may involve relocation or restocking. The same reasoning should be applied to strictly freshwater species that are sessile or have restricted dispersal abilities and are currently imperiled worldwide.
Calcium dobesilate reduces SARS-CoV-2 entry into endothelial cells by inhibiting virus binding to heparan sulfate
Authors: Kiyan Yulia , Schultalbers Anna , Chernobrivaia Ekaterina , Tkachuk Sergey , Rong Song , Shushakova Nelli , Haller Hermann 
Published: October 2022

AbstractRecent reports demonstrate that SARS-CoV-2 utilizes cell surface heparan sulfate as an attachment factor to facilitate the initial interaction with host cells. Heparan sulfate interacts with the receptor binding domain of SARS-CoV-2 spike glycoprotein, and blocking this interaction can decrease cell infection. We and others reported recently that the family of compounds of 2,5-dihydroxyphenylic acid interferes with the binding of the positively charged groove in growth factor molecules to negatively charged cell surface heparan sulfate. We hypothesized that Calcium Dobesilate (CaD)—calcium salt of 2,5-dihydroxyphenylic acid—may also interfere with the binding of SARS-CoV-2 spike protein to heparan sulfate. Using lentiviral SARS-CoV-2 spike protein pseudotyped particles we show that CaD could significantly reduce pseudovirus uptake into endothelial cells. On the contrary, CaD did not affect cell infection with VSVG-expressing lentivirus. CaD could also prevent retention of SARS-CoV-2 spike protein in ex vivo perfused mouse kidney. Using microfluidic culture of endothelial cells under flow, we show that CaD prevents spike protein interaction with heparan sulfate glycocalyx. Since CaD has no adverse side effects and is approved in humans for other medical indications, our findings can rapidly translate into clinical studies.
A real-time rural domestic garbage detection algorithm with an improved YOLOv5s network model
Authors: Jiang Xiangkui , Hu Haochang , Qin Yuemei , Hu Yihui , Ding Rui 
Published: October 2022

AbstractAn increasing number of researchers are using deep learning technology to classify and process garbage in rural areas, and have achieved certain results. However, the existing garbage detection models still have problems such as high complexity, missed detection of small targets, low detection accuracy and poor real-time performance. To address these issues, we train a model and apply it to garbage classification and detection in rural areas. In general, we propose an attention combination mechanism based on the YOLOv5 algorithm to build a better backbone network structure, add a new small object detection layer in the head network to enhance the model's ability to detect small objects, adopt the CIoU loss function to optimize the output prediction bounding box, and choose the Adam optimization algorithm to train the model. Our proposed YOLOv5s-CSS model detects a single garbage image in 0.021 s with a detection accuracy of 96.4%. Compared with the YOLOv5 algorithm and the classic detection algorithm, the improved algorithm has better detection speed and detection accuracy. At the same time, the complexity of the network model is reduced to a certain extent, which can meet the requirements of real-time detection of rural domestic garbage.
Activity of newest generation β-lactam/β-lactamase inhibitor combination therapies against multidrug resistant Pseudomonas aeruginosa
Authors: Haines Robbie R. , Putsathit Papanin , Hammer Katherine A. , Tai Anna S. 
Published: October 2022

AbstractMultidrug resistant (MDR) P. aeruginosa accounts for 35% of all P. aeruginosa isolated from respiratory samples of patients with cystic fibrosis (CF). The usefulness of β-lactam antibiotics for treating CF, such as carbapenems and later generation cephalosporins, is limited by the development of antibacterial resistance. A proven treatment approach is the combination of a β-lactam antibiotic with a β-lactamase inhibitor. New β-lactam/β-lactamase inhibitor combinations are available, but data are lacking regarding the susceptibility of MDR CF-associated P. aeruginosa (CFPA) to these new combination therapies. In this study we determined MIC values for three new combinations; imipenem-relebactam (I-R), ceftazidime-avibactam (CZA), and ceftolozane-tazobactam (C/T) against MDR CFPA (n = 20). The MIC90 of I-R, CZA, and C/T was 64/4, 32/4, and 16/8 (all µg/mL), respectively. The susceptibility of isolates to imipenem was not significantly improved with the addition of relebactam (p = 0.68). However, susceptibility to ceftazidime was significantly improved with the addition of avibactam (p < 0.01), and the susceptibility to C/T was improved compared to piperacillin/tazobactam (p < 0.05) These data provide in vitro evidence that I-R may not be any more effective than imipenem monotherapy against MDR CFPA. The pattern of susceptibility observed for CZA and C/T in the current study was similar to data previously reported for non-CF-associated MDR P. aeruginosa.
The effect of short-term refrigeration on platelet responsiveness
Authors: Kobsar Anna , Koehnlechner Karina , Klingler Philipp , Niklaus Marius , Zeller-Hahn Julia , Koessler Angela , Weber Katja , Boeck Markus , Koessler Juergen 
Published: October 2022

AbstractStorage of platelet concentrates (PC) at cold temperature (CT) is discussed as an alternative to the current standard of storage at room temperature (RT). Recently, we could show that cold-induced attenuation of inhibitory signaling is an important mechanism promoting platelet reactivity. For developing strategies in blood banking, it is required to elucidate the time-dependent onset of facilitated platelet activation. Thus, freshly prepared platelet-rich-plasma (PRP) was stored for 1 and 2 h at CT (2–6 °C) or at RT (20–24 °C), followed by subsequent comparative analysis. Compared to RT, basal and induced vasodilator-stimulated phosphoprotein (VASP) phosphorylation levels were decreased under CT within 1 h by approximately 20%, determined by Western blot analysis and flow cytometry. Concomitantly, ADP- and collagen-induced threshold aggregation values were enhanced by up to 30–40%. Furthermore, platelet-covered areas on collagen-coated slides and aggregate formation under flow conditions were increased after storage at CT, in addition to induced activation markers. In conclusion, a time period of 1–2 h for refrigeration is sufficient to induce an attenuation of inhibitory signaling, accompanied with an enhancement of platelet responsiveness. Short-term refrigeration may be considered as a rational approach to obtain PC with higher functional reactivity for the treatment of hemorrhage.
Nicotinamide adenine dinucleotide reduced (NADH) is a natural UV filter of certain bird lens
Authors: Osik Nataliya A. , Zelentsova Ekaterina A. , Sharshov Kirill A. , Tsentalovich Yuri P. 
Published: October 2022

AbstractIn this work, we for the first time report the identification of UV filters in the bird eye lens. We found that lenses of some raptors (black kite, common buzzard) and waterfowl (birds from Podicipedidae family) contain unusually high levels of reduced nicotinamide adenine dinucleotide (NADH)—a compound with high absorption in the UV-A range with a maximum at 340 nm. The lens metabolome of these birds also features an extremely low [NAD +]/[NADH] ratio. Chemometric analysis demonstrates that the differences between the metabolomic compositions of lenses with low and high NADH abundances should be attributed to the taxonomic features of bird species rather to the influence of the low [NAD +]/[NADH] ratio. We attributed this observation to the low metabolic activity in lens fiber cells, which make up the bulk of the lens tissue. Photochemical measurements show that properties of NADH as a UV filter are as good as that of UV filters in the human lens, including strong absorption in the UV-A spectral region, high photostability under both aerobic and anaerobic conditions, low yields of triplet state, fluorescence, and radicals under irradiation. Lenticular UV filters protect the retina and the lens from photo-induced damages and improve the visual acuity by reducing chromatic aberrations; therefore, the results obtained contribute to our understanding of the extremely high acuity of the raptor vision.
Molecular dynamics on quantum annealers
Authors: Gaidai Igor , Babikov Dmitri , Teplukhin Alexander , Kendrick Brian K. , Mniszewski Susan M. , Zhang Yu , Tretiak Sergei , Dub Pavel A. 
Published: October 2022

AbstractIn this work we demonstrate a practical prospect of using quantum annealers for simulation of molecular dynamics. A methodology developed for this goal, dubbed Quantum Differential Equations (QDE), is applied to propagate classical trajectories for the vibration of the hydrogen molecule in several regimes: nearly harmonic, highly anharmonic, and dissociative motion. The results obtained using the D-Wave 2000Q quantum annealer are all consistent and quickly converge to the analytical reference solution. Several alternative strategies for such calculations are explored and it was found that the most accurate results and the best efficiency are obtained by combining the quantum annealer with classical post-processing (greedy algorithm). Importantly, the QDE framework developed here is entirely general and can be applied to solve any system of first-order ordinary nonlinear differential equations using a quantum annealer.
Impact of anticoagulants on the clinical outcomes of colonic diverticular bleeding comparing warfarin and direct oral anticoagulants
Authors: Chang Li-sa , Nishida Tsutomu , Hosokawa Kana , Fujii Yoshifumi , Osugi Naoto , Sugimoto Aya , Mukai Kaori , Nakamatsu Dai , Matsumoto Kengo , Hayashi Shiro , Yamamoto Masashi , Inada Masami 
Published: October 2022

AbstractRecently, direct oral anticoagulants (DOACs) have been widely used as antithrombotic agents to replace warfarin, but their clinical impact in patients with gastrointestinal bleeding is unclear. We compared the effects of warfarin and DOACs on the outcomes of patients with colonic diverticular bleeding. The patients were divided into warfarin and DOAC groups. We compared the clinical outcomes and the effect of the DOAC dosing and examined any readmissions due to colonic diverticular bleeding within 1 year. A total of 95 events (warfarin group: n = 43 and DOAC group: n = 52) were included. Compared with the warfarin group, the DOAC group was significantly older, had a lower rate of concomitant antiplatelet agents, and a shorter hospital stay, but no significant differences were found in the other clinical outcomes. Thirty-seven patients (71.2%) in the DOAC group had appropriate dosing, whereas 15 patients (28.9%) had an inappropriate dose. The patients with overdose or contraindications had significantly lower minimum hemoglobin levels. In the univariate analysis, prior hospitalization for colonic diverticular bleeding was a significant predictor of readmission. Compared with warfarin, patients with colonic diverticular bleeding treated with DOACs were older and had shorter hospital stays, and the inappropriate use of DOACs may worsen outcomes.
Application of the 2-deoxyglucose scaffold as a new chiral probe for elucidation of the absolute configuration of secondary alcohols
Authors: Trocka Alicja , Szwarc-Karabyka Katarzyna , Makowiec Sławomir , Laskowski Tomasz 
Published: October 2022

AbstractHerein, we present the application of 2-deoxy-d-glucose derivatives as chiral probes for elucidation of the absolute configuration of chiral secondary alcohols. The probes are attached to the studied molecules via glycosylation reaction and the resulting products are examined by a set of standard 2D NMR experiments. The absolute configuration of an oxymethine carbon atom binding the probe is established on a basis of a set of diagnostic dipolar couplings (NOEs/ROEs). These correlations may be considered diagnostic due to a pronounced lack of conformational freedom of the formed glycosidic linkage. While the chance for an observation of the diagnostic signals is the highest when the resulting glycoside in an α-anomer. 2-deoxy-d-glucose was selected as a probe of choice since is it known to strongly prefer the formation of α-glycosides
Lumbar spine intervertebral disc gene delivery of BMPs induces anterior spine fusion in lewis rats
Authors: Cunningham Matthew E. , Kelly Natalie H. , Rawlins Bernard A. , Boachie-Adjei Oheneba , van der Meulen Marjolein C. H. , Hidaka Chisa 
Published: October 2022

AbstractMinimally invasive techniques and biological autograft alternatives such as the bone morphogenetic proteins (BMPs) can reduce morbidity associated with spinal fusions. This study was a proof-of-concept for gene-therapy-mediated anterior spine fusion that could be adapted to percutaneous technique for clinical use. Isogeneic bone marrow stromal cells genetically programmed to express b-galactosidase (LACZ, a marker gene), BMP2, BMP7, a mixture of BMP2 and BMP7 infected cells (homodimers, HM), or BMP2/7 heterodimers (HT) were implanted into the discs between lumbar vertebrae 4 and 5 (L4/5) and L5/6 of male Lewis rats. Spine stiffening was monitored at 4, 8 and 12 weeks using noninvasive-induced angular displacement (NIAD) testing. At 12 weeks isolated spines were assessed for fusion and bone formation by palpation, biomechanical testing [four-point bending stiffness, moment to failure in extension, and in vitro angular displacement (IVAD)], faxitron x-rays, microCT, and histology. Progressive loss of NIAD occurred in only the HT group (p < 0.001), and biomechanical tests correlated with the NIAD results. Significant fusion occurred only in the HT group (94% of animals with one or both levels) as assessed by palpation (p < 0.001), which predicted HT bone production assessed by faxitron (p ≤ 0.001) or microCT (p < 0.023). Intervertebral bridging bone was consistently observed only in HT-treated specimens. Induced bone was located anterior and lateral to the disc space, with no bone formation noted within the disc. Percutaneous anterior spine fusions may be possible clinically, but induction of bone inside the disc space remains a challenge.
Luminous, relativistic, directional electron bunches from an intense laser driven grating plasma
Authors: Lad Amit D. , Mishima Y. , Singh Prashant Kumar , Li Boyuan , Adak Amitava , Chatterjee Gourab , Brijesh P. , Dalui Malay , Inoue M. , Jha J. , Tata Sheroy , Trivikram M. , Krishnamurthy M. , Chen Min , Sheng Z. M. , Tanaka K. A. , Kumar G. Ravindra , Habara H. 
Published: October 2022

AbstractBright, energetic, and directional electron bunches are generated through efficient energy transfer of relativistic intense (~ 1019 W/cm2), 30 femtosecond, 800 nm high contrast laser pulses to grating targets (500 lines/mm and 1000 lines/mm), under surface plasmon resonance (SPR) conditions. Bi-directional relativistic electron bunches (at 40° and 150°) are observed exiting from the 500 lines/mm grating target at the SPR conditions. The surface plasmon excited grating target enhances the electron flux and temperature by factor of 6.0 and 3.6, respectively, compared to that of the plane substrate. Particle-in-Cell simulations indicate that fast electrons are emitted in different directions at different stages of the laser interaction, which are related to the resultant surface magnetic field evolution. This study suggests that the SPR mechanism can be used to generate multiple, bright, ultrafast relativistic electron bunches for a variety of applications.