bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dietary fibre in relation to asthma, allergic rhinitis and sensitization from childhood up to adulthood
Authors: Sdona Emmanouela , Ekström Sandra , Andersson Niklas , Håkansson Niclas , Wolk Alicja , Westman Marit , van Hage Marianne , Kull Inger , Melén Erik , Bergström Anna 
Published: August 2022

AbstractBackgroundDietary fibre may reduce the risk of allergy. Our aim was to investigate the association between fibre intake in childhood, asthma, allergic rhinitis and IgE sensitization up to adulthood.MethodsThe individual fibre intake of 2285 participants from the Swedish population‐based birth cohort BAMSE was estimated between 98‐ and 107‐item food frequency questionnaires at ages 8 and 16 years, respectively. At 8, 16 and 24 years, asthma and allergic rhinitis symptoms were assessed by questionnaires, and sensitization to common allergens by serum IgE. Longitudinal associations were analysed by generalized estimating equations, adjusting for potential confounders.ResultsAn inverse overall association was indicated between fibre intake at 8 years and allergic rhinitis symptoms up to 24 years (OR per 5 g/d 0.86; 95% CI 0.77–0.96), particularly in combination with airborne (0.74; 0.62–0.89) and food (0.69; 0.54–0.88) allergen sensitization. Higher fibre intake was also associated with specific allergen sensitization, for example, birch (0.77; 0.67–0.88) and soy (0.68; 0.53–0.87). No association was observed with asthma. Regarding sources, fruit (0.79; 0.67–0.94) and other (potatoes, chips/popcorn, legumes, and nuts, 0.71; 0.50–0.99), but not cereal or vegetable fibre were associated with allergic rhinitis. In additional analyses, including long‐term fibre intake at 8 and 16 years, excluding participants with food‐related allergic symptoms to examine reverse causation, as well as adjusting for antioxidant intake, associations were attenuated and became non‐significant.ConclusionHigher fibre intake in mid‐childhood may be inversely associated with allergic rhinitis and sensitization to specific allergens up to adulthood. However, avoidance of food triggers of allergic symptoms in allergic rhinitis patients may contribute to the protective associations.
Utility of p16/Ki67 double immunocytochemistry for detection of cervical adenocarcinoma
Authors: Ryu Ayumi , Honma Keiichiro , Shingetsu Azusa , Tanada Satoshi , Yamamoto Takashi , Nagata Shigenori , Kamiura Shoji , Yamasaki Tomoyuki , Ohue Masayuki , Matsuura Nariaki 
Published: August 2022

BackgroundAlthough the incidence of cervical adenocarcinoma has consistently increased, especially among young women, there is no established best means for screening. This study evaluated the screening efficacy of CINtec PLUS (CINtec; p16/Ki67 double immunocytochemistry) expression in cervical glandular cells.MethodsCervical cytology was examined using abnormal glandular cells. The CINtec status of 100 samples with corresponding surgically resected specimens and 11 samples that exhibited negative results for intraepithelial lesion or malignancy at follow‐up was analyzed. Additionally, 31 negative samples containing benign glandular cells were included.ResultsOf the 142 samples, CINtec status was diffusely positive in 74, focally positive in 24, and negative in 44. The 74 diffusely positive samples included 70 adenocarcinomas (62 cervical, seven uterine, and one ovarian) and four cases of high‐grade cervical intraepithelial neoplasia. The 24 focally positive samples included 15 adenocarcinomas (seven cervical, seven uterine, and one fallopian tube) and nine without malignancy. The 44 negative samples included nine adenocarcinomas (five uterine and four cervical) and 35 without malignancy. The sensitivity, specificity, positive predictive value, and negative predictive value of the CINtec diffusely or focally positive cases for cervical adenocarcinomas were 94.5%, 58.0%, 70.4%, and 90.9%, respectively. In CINtec diffusely positive cases, the respective values were 84.9%, 82.6%, 83.8%, and 83.8%, and in women aged ≤39 years the values were 90.6%, 89.5%, 93.5%, and 85.0%, respectively.ConclusionsCINtec may support efficient detection of cervical adenocarcinomas.
Small sensory spinal lesions that affect hand function in monkeys greatly alter primary afferent and motor neuron connections in the cord
Authors: Fisher Karen M. , Garner Joseph P. , Darian‐Smith Corinna 
Published: August 2022

AbstractSmall sensory spinal injuries induce plasticity across the neuraxis, but little is understood about their effect on segmental connections or motor neuron (MN) function. Here, we begin to address this at two levels. First, we compared afferent input distributions from the skin and muscles of the digits with corresponding MN pools to determine their spatial relationship, in both the normal state and 4–6 months after a unilateral dorsal root/dorsal column lesion (DRL/DCL), affecting digits 1–3. Second, we looked at specific changes to MN inputs and membrane properties that likely impact functional recovery. Monkeys received a targeted unilateral DRL/DCL, and 4–6 months later, cholera toxin subunit B (CT‐B) was injected bilaterally into either the distal pads of digits 1–3, or related intrinsic hand muscles, to label inputs to the cord, and corresponding MNs. In controls (unlesioned side), cutaneous and proprioceptive afferents from digits 1–3 showed different distribution patterns but similar rostrocaudal spread within the dorsal horn from C1 to T2. In contrast, MNs were distributed across just two segments (C7–8). Following the lesion, sensory inputs were significantly diminished across all 10 segments, though this did not alter MN distributions. Afferent and monoamine inputs, as well as KCC2 cotransporters, were also significantly altered on the cell membrane of CT‐B labeled MNs postlesion. In contrast, inhibitory neurotransmission and perineuronal net integrity were not altered at this prechronic timepoint. Our findings indicate that even a small sensory injury can significantly impact sensory and motor spinal neurons and provide new insight into the complex process of recovery.
Does corporate environmental responsibility contribute to financial performance? A dual path analysis through operational efficiency and the cost of debt
Authors: Gao Lan , Wan Liang 
Published: August 2022

AbstractGiven the growing environmental awareness among stakeholders, firms are increasingly been asked to act in an environmentally responsible manner. However, the relationship between corporate environmental responsibility (CER) and corporate financial performance is quite mixed. This study aims to examine the impact of CER on financial performance and explores the mediating effects of operational efficiency and the cost of debt. A firm‐level panel data of 2173 Chinese listed firms are analyzed. The results show that efforts and investments in CER positively and directly affect financial performance. Operational efficiency and cost of debt mediate the relationship between CER and financial performance. This study contributes to the stakeholder theory and highlights that CER can promote corporate financial performance by improving operational efficiency and reducing the cost of debt. This study also has practical implications and highlights the importance of enhancing operational management and information communication in the course of CER practices.
Palmatine attenuates hepatocyte injury by promoting autophagy via the AMPK/mTOR pathway after alcoholic liver disease
Authors: Lin Guo‐Shuai , Zhao Mao‐Mao , Fu Qi‐Chao , Zhao Shu‐Yi , Ba Tao‐Tao , Yu Hong‐Xia 
Published: August 2022

AbstractAlcoholic liver disease is one of the diseases with the highest fatality rate worldwide. The cellular process of autophagy which recycles damaged organelles to maintain protein and organelle homeostasis is found to positively influence survival during hepatic insufficiency, although the mechanism is poorly understood. Palmatine (PLT) has a variety of biological functions, such as broad‐spectrum antibacterial action, neuroprotective, antioxidant stress, and antiviral and anti‐inflammatory activities. However, it is not known whether PLT has a protective effect against alcoholic liver injury. Here, we investigated the protective effect of PLT in a cellular model of alcohol‐induced acute liver injury and further explored its mechanism of action. In this study, we show for the first time that PLT attenuates alcohol‐induced hepatocyte injury by promoting autophagy to play an essential protective role. As PLT treatment induced a brief increase in LC3‐II conversion and p62 degradation, it also upregulated the expression of ATG5 and ATG7. The expression levels of the proapoptotic proteins Bax, Caspase 3, and Caspase 9 significantly decreased, while the antiapoptotic protein levels of Bcl‐2 upregulated after treatment with PLT. However, in presence of the autophagy inhibitor, 3‐methyladenine, the effect of PLT in inhibiting ethanol‐induced hepatocyte injury reversed significantly. Mechanistically, the protective effects of PLT may be mediated by promoting the activation of the AMP‐activated protein kinase/mammalian target of rapamycin signaling pathway. Therefore, we believe that the development of alcoholic liver injuries may be controlled by PLT by inhibiting hepatocyte apoptosis through the autophagy pathway. The study lays a solid theoretical and practical basis for future animal models and clinical studies of PLT.
Synthesis and characterization of d5‐barbarin for use in barbarin‐related research
Authors: Kudrimoti Sucheta , Machin Jacob , Arojojoye Adedamola S. , Awuah Samuel G. , Eisenberg Rodney , Fenger Clara , Maylin George , Lehner Andreas F. , Tobin Thomas 
Published: August 2022

AbstractBased on structural similarities and equine administration experiments, Barbarin, 5‐phenyl‐2‐oxazolidinethione from Brassicaceae plants, is a possible source of equine urinary identifications of aminorex, (R,S)‐5‐phenyl‐4,5‐dihydro‐1,3‐oxazol‐2‐amine, an amphetamine‐related US Drug Enforcement Administration (DEA) controlled substance considered illegal in sport horses. We now report the synthesis and certification of d5‐barbarin to facilitate research on the relationship between plant barbarin and such aminorex identifications. D5‐barbarin synthesis commenced with production of d5‐2‐oxo‐2‐phenylacetaldehyde oxime (d5‐oxime) from d5‐acetophenone via butylnitrite in an ethoxide/ethanol solution. This d5‐oxime was then reduced with lithium aluminum hydride (LiAlH4) to produce the corresponding d5‐2‐amino‐1‐phenylethan‐1‐ol (d5‐phenylethanolamine). Final ring closure of the d5‐phenylethanolamine was performed by the addition of carbon disulfide (CS2) with pyridine. The reaction product was purified by recrystallization and presented as a stable white crystalline powder. Proton NMR spectroscopy revealed a triplet at 5.88 ppm for one proton, a double doublet at 3.71 ppm for one proton, and double doublet at 4.11 ppm for one proton, confirming d5‐barbarin as the product. Further characterization by high resolution mass spectrometry supports the successful synthesis of d5‐barbarin. Purity of the recrystallized product was ascertained by High Performance Liquid Chromatography (HPLC) to be greater than 98%. Together, we have developed the synthesis and full characterization of d5‐barbarin for use as an internal standard in barbarin‐related and equine forensic research.
IL‐33/ST2 pathway as upper‐hand of inflammation in allergic asthma contributes as predictive biomarker in heart failure
Authors: Mehrabi Nasab Entezar , Hassanzadeh Makoei Reza , Aghajani Hassan , Athari Seyyed Shamsadin 
Published: August 2022

AbstractAllergic asthma is an inflammatory disorder of the bronchi, and as a major health problem, more than 350 million people suffer from asthma in the world. Many cardiovascular disorders resulted in the impairment of the heart's power to pump blood that leads to the HF. More than 25 million people worldwide live with HF. Accordingly, identifying the biomarkers to predict the onset of future asthma and HF is necessary. IL‐33 is an inflammatory cytokine that has the main role in pathophysiology of asthma and HF. Also, in IL‐33 receptor, the ST2 is involved in cardiac fibrosis and remodelling in HF and pathogenesis of allergic asthma. Increased sST2 in allergic asthma helps to control inflammation during asthma, but increased sST2 in HF is a predictable biomarker to present risk factor of HF during the time of the patients.
Humanized mice for investigating SARS‐CoV‐2 lung infection and associated human immune responses
Authors: Sun Renren , Zhao Zongzheng , Fu Cong , Wang Yixin , Guo Zhendong , Zhang Chunmao , Liu Lina , Zhang Cheng , Shu Chang , He Jin , Yang Yong‐Guang , Hua Shucheng , Gao Yuwei , Hu Zheng 
Published: August 2022

AbstractThere is an urgent need for animal models of coronavirus disease 2019 to study immunopathogenesis and test therapeutic intervenes. In this study, we showed that NOD/SCID IL2rg–/– (NSG) mice engrafted with human lung (HL) tissue (NSG‐L mice) could be infected efficiently by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), and that live virus capable of infecting Vero cells was found in the HL grafts and multiple organs from infected NSG‐L mice. RNA‐Sequencing identified a series of differentially expressed genes, which are enriched in viral defense responses, chemotaxis, IFN stimulation and pulmonary fibrosis, between HL grafts from infected and control NSG‐L mice. Furthermore, when infected with SARS‐CoV‐2, humanized mice with both human immune system (HIS) and autologous HL grafts (HISL mice) had bodyweight loss and hemorrhage and immune cell infiltration in HL grafts, which were not observed in immunodeficient NSG‐L mice, indicating the development of anti‐viral immune responses in these mice. In support of this possibility, the infected HISL mice showed bodyweight recovery and lack of detectable live virus at the later time. These results demonstrate that NSG‐L and HISL mice are susceptible to SARS‐CoV‐2 infection, offering a useful in vivo model for studying SARS‐CoV‐2 infection and the associated immune response and immunopathology, and testing anti‐SARS‐CoV‐2 therapies.
Crystalline Assembly and Solvent‐induced Solid‐state Transformation of 1D Zigzag Chains Based on Sulfonylcalix[4]arene Trinuclear Co(II) and Zn(II) Clusters
Authors: Kniazeva Mariia V. , Ovsyannikov Alexander S. , Islamov Daut R. , Samigullina Aida I. , Gubaidullin Aidar T. , Dorovatovskii Pavel V. , Solovieva Svetlana E. , Antipin Igor S. , Ferlay Sylvie 
Published: August 2022

AbstractThe reproducible formation, in mild conditions, of three new 1D zigzag coordination polymers using sulfonylcalix[4]arene (TCASO2), metal M2+ cations (M=Co or Zn) and rigid planar N‐shaped linkers (stilbene‐4,4′‐dicarboxylic acid (1‐2H) or azobenzene‐4,4′‐dicarboxylic acid (2‐2H)) is reported. The obtained coordination compounds (1‐TCASO2Zn3, 1‐TCASO2Co3 and 2‐TCASO2Zn3) are characterized in the crystalline phase using single crystal and powder X‐ray diffraction (XRD and XRPD), TGA and IR‐spectroscopy. The three compounds present the same coordination pattern and 1‐TCASO2Zn3 and 2‐TCASO2Zn3 reveal to be isostructural. Due to a high porosity and amount of accommodated solvent molecules hosted in the network, the generated rigid 1D chains are capable to undergo a reversible dynamic solid‐state transformation in terms of crystal packing, accompanied with release/uptake of DMSO molecules leading to compression/stretching of the unit cell along one of the crystallographic axis which was attested by Rietveld refinement on XRPD data.
The association between serum estradiol levels and sperm DNA integrity
Authors: Lu Viktor , Svensjö Oscar , Axelsson Jonatan 
Published: August 2022

AbstractIn men from the general population, BMI has been associated with a lower sperm DNA fragmentation index (DFI). We wondered whether this could be due to estradiol, which is associated with BMI and reported important for sperm function. Our objective was to investigate the association between estradiol and DFI. In 2008–2010, we recruited 284 young men from the general population to deliver samples of semen and blood and answer questionnaires. Serum concentrations of reproductive hormones and DFI were analyzed, the latter using the Sperm Chromatin Structure Assay. Associations were studied using general linear models. The first model utilized metric values of estradiol, whereas the second model compared men with high and low levels, dichotomized by the median value. A possible interaction between estradiol and testosterone was also examined. When investigating metric estradiol levels and DFI, an inverse association was seen without adjustments (p = .02), but the statistical significance was lost at adjustments for potential confounders (p = .08). Men with lower estradiol levels (<88 pmol/L, mean 71 pmol/L) had a statistically significantly higher DFI than men with higher levels of estradiol (≥88 pmol/L, mean 110 pmol/L). Mean ratio difference was 1.21 (p = .002) without adjustments and 1.18 (p = .01) with adjustments. A statistically significant difference in DFI was observed in men with testosterone levels below median when comparing high and low estradiol (p < .001). This study supports the idea that serum estradiol levels are protective for sperm DNA integrity, at least at lower testosterone levels.
Responses of Wild Finescale Dace (Phoxinus neogaeus) to Experimental Spills of Cold Lake Blend Diluted Bitumen at the International Institute for Sustainable Development-Experimental Lakes Area, Northwestern Ontario
Authors: Timlick Lauren , Dearnley Jamie , Blais Jules M. , Rodríguez-Gil José L. , Hanson Mark , Hollebone Bruce P. , Orihel Diane M. , Peters Lisa E. , Stoyanovich Sawyer S. , Palace Vince P. 
Published: August 2022

Abstract
Pipelines carrying diluted bitumen (dilbit) traverse North America and may result in dilbit release into sensitive freshwater ecosystems. To better understand the potential effects of a freshwater oil release, the Boreal-lake Oil Release Experiment by Additions to Limnocorrals project at the International Institute for Sustainable Development-Experimental Lakes Area (Ontario, Canada) modeled seven dilbit spills contained within a 10-m diameter of littoral limnocorrals in a boreal lake. Wild finescale dace (Phoxinus neogaeus) were released in the limnocorrals 21 days after oil addition and remained there for 70 days. Dilbit volumes covered a large range representing a regression of real spill sizes and total polycyclic aromatic compounds (TPAC) between 167 ng L−1 day−1 and 1989 ng L−1 day−1. We report the effects of chronic exposure on reproductive potential as well as physiological responses in the gallbladder and liver. In exposures &gt;1000 ng L−1 day−1, there was a significant decrease in fish retrieval, culminating in zero recapture from the three highest treatments. Among the fish from the limnocorrals with lower levels of TPAC (&lt;500 ng L−1 day−1), effects were inconsistent. Gallbladder bile fluorescence for a naphthalene metabolite was significantly different in fish from the oil-exposed limnocorrals when compared to the lake and reference corral, indicating that fish in these lower exposures were interacting with dilbit-derived polycyclic aromatic compounds. There were no significant differences in condition factor, somatic indices, or hepatocyte volume indices. There were also no significant changes in the development of testes or ovaries of exposed dace. The results from the present study may serve to orient policymakers and emergency responders to the range of TPAC exposures that may not significantly affect wild fish. Environ Toxicol Chem 2022;41:2745–2757. © 2022 SETAC
Expansion of the Conceptual Model for the Accumulation of Selenium in Lentic Food Chains to Include Redox-Controlled Generation and Diffusion of Selenite and Dissolved Organo-Selenium Compounds
Authors: Martin Alan J. , Kuang Cheng , Wallschläger Dirk 
Published: August 2022

Abstract
The controls governing the availability of reduced selenium (Se) species, namely selenite (Se[IV]) and dissolved organo-Se (DOSe), to primary producers at the sediment–water interface in depositional environments (i.e., lentic systems) were assessed through consideration of theoretical principles and field data. Selenite is generated in suboxic sediment porewater via the microbially mediated reduction of selenate (Se[IV]) and/or reductive dissolution of Se-bearing iron oxides. Field data for lentic environments demonstrate that the production of DOSe in sediment porewaters can also be redox- and depth-dependent. In this manner, the remobilization depths of Se(IV) and DOSe in depositional environments are dependent on the vertical redox gradient (dEh/dz), where deeper depths of remobilization are observed in less reducing sedimentary environments (lower dEh/dz). In turn, remobilization depth has a direct bearing on the concentration of dissolved Se(IV) and DOSe that may be realized at the sediment–water interface because the depth of reaction governs the diffusive path length, concentration gradient, and rate of diffusional transport toward the sediment–water interface. The principles that link sediment redox gradients, depth of remobilization, diffusive transport processes, and concentration of reduced Se species at the sediment–water interface have a direct bearing on the potential for Se uptake by primary producers in lentic food chains (e.g., phytoplankton, biofilms, bacteria). Overall, these processes complement the current conceptual “benthic detrital food chain” model that describes the accumulation of Se in lentic systems. Environ Toxicol Chem 2022;41:2859–2869. © 2022 SETAC
The effects of biogenic amines in Chinese Huangjiu on the behavior of mice and hangover headache‐related indices
Authors: Zhao Wenmei , Fu Zukang , Wang Xin , Mao Qingzhong , Luan Chunguang , Chen Shanbin , Zhang Fengjie , Yu Jiajun , Yao Yiping , Li Yishu , Hao Feike , Wang Deliang , Li Nan , Huangfu Jie , Wang Chengtao 
Published: August 2022

AbstractHuangjiu (Chinese rice wine) is a popular and traditional alcoholic beverage in China; however, the consumption of Huangjiu readily results in hangover symptoms. The aim of this study was to identify the main components associated with behavioral inhibition, headache, and the relevant mechanisms by using a mice hangover model. The results of an open‐field experiment revealed that the key biogenic amine associated with mice behavior was histamine, which inhibited the behavior activity of mice in a dose‐dependent manner. Moreover, histamine treatment decreased the levels of serotonin (5‐HT) and 5‐hydroxyindole acetic acid. In addition, the levels of dopamine and nitric oxide, which are associated with migraine, increased in the brain tissue of mice. In addition, the expression of receptor genes of 5‐HT, including Htr1a, Htr1f, and Htr2c, is essential in regulating various behaviors and mental activities. In conclusion, the present study demonstrated that histamine is a key component in Huangjiu, and it is related to hangover symptoms by affecting the level of 5‐HT and its receptors.