bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utilization of Yam Bean Juice as Nutrient Source for Bacterial Cellulose
Production by Komagataeibacter nataicola TISTR 975
Authors: Chaiyachet Orn Anong , Thongmoon Sucheera , Udomchai Tharintorn 
Published: September 2022

Background:
Bacterial cellulose has attracted much interest over the years because of
its diverse applications stemming from its unique properties. Alternative sources of raw materials
for culture medium have become essential to reduce the cost of raw materials and scale up bacterial
cellulose production.

Objectives:
The present study aims to investigate the cellulose-producing ability and characteristics
of bacterial cellulose produced by Komagataeibacter nataicola TISTR 975 using yam bean
juice as a nutrient source and optimizing the culture medium conditions to produce bacterial cellulose.

Methods:
Bacterial cellulose was produced by K. nataicola TISTR 975 using yam bean juice as
the nutrient source in the culture medium. Fermentation was performed in static culture using
10% inoculum, with varying levels of initial total soluble solids content (8, 10, and 12 °Brix),
supplemented with ammonium sulfate (0.1, 0.3, and 0.5 (% w/v)), pH 5.0, 1.4% (v/v) ethanol,
and incubated at 30 °C for 10 days. The cellulose membrane was measured for cellulose yield.
Physicochemical and sensory characteristics of bacterial cellulose were examined.

Results:
Initial total soluble solids content at 10 °Brix (equal to the sugar content of approximately
100 g/L) and supplemented with 0.1% (w/v) of ammonium sulfate improved bacterial
cellulose yield. Moisture content and water holding capacity (WHC) of bacterial cellulose were
high, and L*, a*, and b* values and textural properties were related to perceived sensory characteristics.
Sensory evaluation showed the highest score for color and overall acceptability.

Conclusion:
Local edible yam bean tubers could be used as an alternative raw material for bacterial
cellulose production by K. nataicola TISTR 975 using yam bean juice, and bacterial cellulose
produced using yam bean juice as a nutrient source has the sensory attributes consistent with
the desirable characteristics of raw material for food and processed food products.
Identification of Virulence Markers and Phylogenetic Groups’ Association,
and Antimicrobial Susceptibility of Uropathogenic Escherichia coli
Isolates
Authors: Meziani Dahbia Yasmina , Barnich Nicolas , Boucheham Anouar , Rezgoune Mohamed larbi , Benlabed Kaddour , Rodrigues Michael , Satta Dalila 
Published: September 2022

Background:
Urinary tract infections represent a world public health problem, which is
caused mainly by Uropathogenic Escherichia coli. Although they are originally found in the intestinal
microbiota in the majority of the cases, urinary tract infections can also be caused by intra-intestinal
pathogenic E. coli.

Objective:
The main objective of our research is to identify the virulence factors generally associated
with different pathotypes across phylogenetic groups.

Methods:
E. coli were isolated from patients with urinary tract infections. Antimicrobial susceptibility
tests, virulence genes and phylogroups were prospected. The data analysis were performed using the
chi-square and Fisher exact test.

Results:
In total, 72.2% of isolates showed multidrug resistant. We have also depicted an important
association between E. coli from inpatients with UTIs and pap and hlyA genes (p-0.041 and p-0.019
respectively). The predominant phylogenetic group in our isolates is B2 (45.4%) followed by D
(12.4%). Our results showed that 9.3% of isolates have an unknown phylogroup which shows a significant
association with astA gene (p-0.008). We have as well found a significant association between B2
and three virulence genes namely pap, hlyA and invE (p-0.002, p-0.001, p-0.025 respectively); B1 and
pap, hlyA genes (p-0.049 and p-0.021 respectively); E and afa gene (p-0.024).

Conclusion:
Certain virulence factors have been shown to be potential targets for drug design and
therapeutic pathways in order to deal with the antimicrobial resistance problem enhanced by antibiotic
therapy.
The Edible Seaweed Gelidium amansii Promotes Structural Plasticity of
Hippocampal Neurons and Improves Scopolamine-induced Learning and
Memory Impairment in Mice
Authors: Mohibbullah Md. , Pringgo Wicaksono Michael Yordan , Hannan Md. Abdul , Dash Raju , Nur Meinita Maria Dyah , Choi Jae-Suk , Hong Yong-Ki , Moon Il Soo 
Published: September 2022

Background:
Gelidium amansii has been gaining profound interest in East Asian countries
due to its enormous commercial value for agar production and its extensive pharmacological properties.
Previous studies have shown that the ethanol extract of Gelidium amansii (GAE) has promising
neurotrophic effects in in vitro conditions.

Objectives:
The present study aimed to investigate the protective effects of GAE against scopolamineinduced
cognitive deficits and its modulatory effects on hippocampal plasticity in mice.

Methods:
For memory-related behavioral studies, the passive avoidance test and radial arm maze paradigm
were conducted. The brain slices of the hippocampus CA1 neurons of experimental mice were
then prepared to perform Golgi staining for analyzing spine density and its characteristic shape and
immunohistochemistry for assessing the expression of different pre- and postsynaptic proteins.

Results:
Following oral administration of GAE (0.5 mg/g body weight), mice with memory deficits
exhibited a significant increase in the latency time on the passive avoidance test and a decrease in the
number of working and reference memory errors and latency time on the radial arm maze test. Microscopic
observations of Golgi-impregnated tissue sections and immunohistochemistry of hippocampal
slices showed that neurons from GAE-treated mice displayed higher spine density and spine dynamics,
increased synaptic contact, and the recruitment of memory-associated proteins, such as N-methyl-Daspartate
receptors (NR2A and NR2B) and postsynaptic density-95 (PSD-95) when compared with the
control group.

Conclusion:
With these memory-protective functions and a modulatory role in underlying memoryrelated
events, GAE could be a potential functional food and a promising source of pharmacological
agents for the prevention and treatment of memory-related brain disorders.
Antihyperglycemic Effect of Aqueous Extract of Tetraclinis articulata in
Streptozotocin-Induced Diabetic Rats and Acute Toxicity Analysis
Authors: Eddouks Mohamed , Bouadid Ismail , Akdad Mourad 
Published: September 2022

Aims:
The study aimed to evaluate the glucose-lowering effect of Tetraclinis articulata.

Background:
Tetraclinis articulata is commonly used for the treatment of diabetes characterized by chronic hyperglycemia.

Objective:
This work aimed to evaluate the effect of Tetraclinis articulata (T. articulata) aqueous extract (TAAE) on glycaemia and lipid profile in normal and streptozotocin(STZ)-induced diabetic rats. Additionally, its acute toxicity, phytochemical composition, and antioxidant capacity were assessed.

Methods:
To highlight the effect of TAAE on plasma glucose levels and lipid metabolism, blood glucose levels were measured at 1, 2, 4, and 6 hours of treatment for the acute test and on days 2, 4 and 7 over the daily oral administration for the subchronic test at two selected doses (10 mg/kg and 20 mg/kg). Furthermore, Triglycerides (TGs), total cholesterol (TC), and High-density lipoprotein cholesterol (HDL-c) were measured after the treatment. The rats' liver, extensor digitorum longus (EDL), and soleus muscle were isolated from diabetic rats treated with TAAE at a dose of 20 mg/kg at the end of the experiment to measure glycogen content using a standard method. The acute toxicity of TAAE was examined according to the OECD guideline. In addition, body weight, signs of toxicity, and/or mortality were observed for 14 days. Besides, a preliminary phytochemical screening, quantification of phenolic, flavonoid, and tannin contents as well as the antioxidant activity were evaluated.

Results:
The results showed that TAAE at the doses of 10 and 20 mg/kg possesses a potent antihyperglycemic effect in STZ-treated diabetic rats and an acute hypoglycemic effect in normal rats, as well as, the extract provoked a decrease of blood glucose levels after glucose loading in the glucose tolerance test in a dose-dependent manner. TAAE at a dose of 20 mg/kg revealed a significant improvement of the lipid profile. However, treatment with TAAE at a dose of 20 mg/kg did not significantly modify the glycogen content. In the same way, the acute toxicity analysis revealed no death or signs of toxicity in rats, and the LD50 value was more than 2 g/kg. In addition, preliminary phytochemical screening revealed that TAAE revealed the presence of polyphenols, flavonoids, tannins, carbohydrates, saponins, quinones, sterols and terpenoids. Furthermore, TAAE exhibited a potent antioxidant activity which may be due to the richness in polyphenol content (756.21±6.72 mg GAE/1 g of extract).

Conclusion:
The current study demonstrates for the first time that aqueous Tetraclinis articulata extract has a potent the glucose-lowering effect.
Tert-butylhydroquinone Mitigates Renal Dysfunction in Pregnant Diabetic
Rats via Attenuation of Oxidative Stress and Modulation of the iNOs/
NFkB/TNF Alpha Signalling Pathway
Authors: Li Jia , Liu Haiying , Shang Li 
Published: September 2022

Aim:
To determine the effect of tert-butylhydroquinone (tBHQ) supplementation on some biochemical parameters in pregnant diabetic rats and the foetus.

Background:
The global incidence of gestational diabetes mellitus (GDM) has been on the increase despite current interventional therapies, underscoring the need for alternative or complementary therapeutic approaches.

Objective:
To determine the effect of tBHQ on blood glucose, insulin resistance, body weights, relative liver and kidney weights, serum lipid profile (total cholesterol, triacylglycerol, high density lipoprotein cholesterol and very low density lipoprotein cholesterol), liver function (albumin, aspartate and alanine amino transaminases) and kidney function markers (urea, creatinine and uric acid) in the sera, maternal and foetal renal levels of oxidative stress and inflammatory markers, foetal weights and histology of the kidney of streptozotocin (STZ) induced GDM in rats was studied.

Methods:
Twenty female pregnant rats were used and they were divided into four groups of five rats each made up of control (normal pregnancy), disease (diabetic untreated), metformin (received 200 mg/kg metformin dissolved in distilled water) and tBHQ groups (received 25 mg/kg tBHQ in 1% corn oil) respectively. GDM was induced in the disease and treated groups by intraperitoneal injection of STZ (45 mg/kg in sodium citrate buffer, pH 4.5).

Results:
STZ induction in the disease group, significantly increased their blood glucose levels (P<0.05), altered their body and foetal weights, relative liver and kidney weights, serum lipid profile, liver and kidney function markers in the sera (relative to the control), inducing oxidative stress and inflammation to the maternal and foetal kidneys and altered the maternal kidney histology which was improved following supplementation with tBHQ in a manner akin to or ever better than metformin.

Conclusion:
tBHQ was found beneficial in protecting the foetal kidneys against oxidative stress and the foetus against mortality arising from maternal hyperglycaemia. Finally, the study showed the potentials of tBHQ in mitigating histological changes in the maternal kidney arising from STZ induced hyperglycemia in rats.
Solvatochromic, Photophysical and DFT Studies of Newer 1-(2-
oxo-2-(2-oxo-2H-chromen-3-yl)ethyl)pyridin-1-ium Bromide and
1-methyl-3-(2-oxo-2-(2-oxo-2H-chromen-3-yl)ethyl)-1H-imidazol-
3-ium Bromide Synthesized under Microwave Irradiation
Authors: Mahulikar Pramod P. , Patil Atul S. , Patil Raosaheb S. , Patil Vikas S. 
Published: September 2022

Aim:
The aim of the study was to design and develop a greener, efficient protocol towards
newer pyridinium and imidazolium compounds, and investigate optical, solvatochromic,
thermal, and theoretical properties.

Background:
Pyridinium and imidazolium based compounds show diverse application compounds with these basic skeleton designs in respect to get improved optical and thermal behavior.

Objectives:
The purpose is to study the optical properties of pyridinium and imidazolium
compounds, for which we illustrate the solvent polarity effect on the absorption and emission
behavior as a function of orientation polarizability and ET(30) solvent parameters. The
study focuses on thermal stability and computes the molecular orbital orientation and HOMO-
LUMO energies using theoretical simulation by the DFT approach.

Methods:
The structures were confirmed by FT-IR, Mass, 1H NMR and 13C NMR, and optical
properties were investigated using a UV-Visible spectrophotometer and fluorometer.
The thermal behavior was investigated using thermal gravimetric analysis, and molecular
orbital orientation and energies were determined using GAUSSIAN 16 software.

Results:
The newer compounds with good thermal stability and optical behavior have been
synthesized and characterized. The study interprets the intermolecular electron transfer
amongst the molecules and the effect of solvents on their excitation and emission properties.
The experimental and theoretical study illustrates the optical, thermal, and electronic
properties of both compounds.

Conclusion:
The present work describes the solvatochromic optical behavior of pyridinium
bromide and imidazolium bromide synthesized by a microwave-assisted, greener and efficient
strategy. The solvatochromic study interprets the presence of non-specific solutesolvent
interactions. The photophysical, thermal, and DFT study revealed that both pyridinium
and imidazolium compounds are used for optoelectronic applications. Moreover, the
work could be helpful to researchers for developing new skeletons for optoelectronic applications.
Novel and existing flexible survival methods for network meta-analyses
Authors: Heeg Bart , Garcia Andrea , Beekhuizen Sophie van , Verhoek Andre , Oostrum Ilse van , Roychoudhury Satrajit , Cappelleri Joseph Christopher , Postma Maarten Jacobus , Nicolaas Martinus Ouwens Mario Johannes 
Published: September 2022

Aim:Technical Support Document 21 discusses trial-based, flexible relative survival models. The authors generalized flexible relative survival models to the network meta-analysis (NMA) setting while accounting for different treatment-effect specifications.Methods:The authors compared the standard parametric model with mixture, mixture cure and nonmixture cure, piecewise, splines and fractional polynomial models. The optimal treatment-effect parametrization was defined in two steps. First, all models were run with treatment effects on all parameters and subsequently the optimal model was defined by removing uncertain treatment effects, for which the parameter was smaller than its standard deviation. The authors used a network in previously treated advanced non-small-cell lung cancer.Results:Flexible model-based NMAs impact fit and incremental mean survival and they increase corresponding uncertainty. Treatment-effect specification impacts incremental survival, reduces uncertainty and improves the fit statistic.Conclusion:Extrapolation techniques already available for individual trials can now be used for NMAs to ensure that the most plausible extrapolations are being used for health technology assessment submissions.
Endotrophin as a Marker of Complications in a Type 2 Diabetes Cohort
Authors: Tougaard Ninna Hahn , Møller Alexandra Louise , Rønn Pernille Falberg , Hansen Tine Willum , Genovese Federica , Karsdal Morten Asser , Rasmussen Daniel Guldager Kring , Rossing Peter 
Published: September 2022

OBJECTIVE
We investigated endotrophin, a profibrotic signaling molecule reflecting collagen VI formation, in serum and urine as risk marker for complications to type 2 diabetes.

RESEARCH DESIGN AND METHODS
Endotrophin was measured in 774 individuals with type 2 diabetes. Outcomes included a composite kidney end point, first major adverse cardiovascular event (MACE), mortality, progression of albuminuria, incident heart failure, and sight-threatening eye disease. Adjusted Cox proportional hazards models were applied.

RESULTS
Doubling of serum endotrophin was associated with the kidney end point (n = 49; hazard ratio 1.80 [95% CI 1.13–2.87]), first MACE (n = 66; 1.54 [1.04–2.28]), mortality (n = 156; 1.69 (1.31–2.19]), and incident heart failure (n = 42; 1.63 [1.02–2.60]). A doubling of urine endotrophin was associated with progression of albuminuria (n = 85; 1.20 [1.04–1.39]).

CONCLUSIONS
Serum endotrophin was a risk marker for mortality and kidney and cardiovascular complications in type 2 diabetes. Urine endotrophin was a marker for albuminuria progression.
Exposure to Gestational Diabetes Mellitus Prior to 26 Weeks is Related to The Presence of Mediobasal Hypothalamic Gliosis in Children
Authors: Chandrasekaran Suchitra , Melhorn Susan , Olerich Kelsey LW , Angelo Brendan , Chow Ting , Xiang Anny , Schur Ellen A. , Page Kathleen A. 
Published: September 2022

<p>Intrauterine exposure to metabolic dysfunction leads to offspring metabolic dysfunction in human and rodent models, but underlying mechanisms are unclear. The mediobasal hypothalamus (MBH) is involved in energy homeostasis and weight regulation, and MBH gliosis associates with obesity and insulin resistance. We tested the hypothesis that offspring exposed to gestational diabetes mellitus (GDM) <em>in utero</em> <em>vs</em> those unexposed would demonstrate evidence of MBH gliosis. Participants in the BrainChild Study (age 7-11 years, with confirmed GDM exposure or no GDM exposure) underwent brain magnetic resonance imaging to acquire T2 weighted images. By using the amygdala (AMY) and white matter (WM) as reference regions, MBH:AMY and MBH:WM T2 signal ratios were calculated as a radiologic measure of MBH gliosis. Linear regressions examined associations between GDM exposure (GDM overall, and by timing of GDM exposure (<u>£</u>26 weeks or >26 weeks) and MBH gliosis. Secondary analyses examined associations between pre-pregnancy BMI and child MBH gliosis. There were no differences in T2 signal ratios in children exposed vs unexposed to GDM overall, but children exposed to early GDM (<u><</u>26 weeks gestation) had higher MBH:WM ratios compared to unexposed (β=0.147, SE 0.06; p=0.03), adjusting for child’s age, sex, BMI z-score and maternal pre-pregnancy BMI; whereas no associations were seen for the control ratio. Pre-pregnancy BMI was not associated with evidence of MBH gliosis. Early exposure to GDM was associated with radiologic evidence of MBH gliosis in children. These data provide mechanistic insight into brain pathways by which exposure to GDM may increase risk for metabolic dysfunction. </p>
The Prognostic Value of the Geriatric Index of Comorbidity for Predicting an Outcome in Patients Over 60 Years of Age With Polytrauma. Retrospective Cohort Study
Authors: Valieva R. I. , Zadneprovskiy N. N. , Ivanov P. A. 
Published: September 2022

Background Nowadays, since the older people are keeping and increasing their professional and social activity, the risk of injury in these patients is escalating. However, the results of polytrauma treatment among patients over 60 years old are worse than in the group of younger patients. This may be associated with the presence of concomitant somatic pathology, which worsens the prognosis of for survival among elderly and senile patients. In the modern literature, there is no description of effective tools for assessing the comorbid status in elderly and senile patients with polytrauma. Nevertheless, there are scales and indices of comorbidity developed for non-surgical patients. To increase the effectiveness of treatment of patients over 60 years of age with polytrauma and a several concomitant diseases, it is necessary to develop new or adapt one of the already created tools for assessing comorbid status.Purpose of the study To assess the dependence of the mortality rate in a group of patients with polytrauma over 60 years of age on the presence of concomitant somatic pathology and its severity, calculated by the geriatric index of comorbidity.Materia l and methods During the period from 2005 to 2020, 116 patients with polytrauma and chronic somatic pathology were treated at the Sklifosovsky Institute. The inclusion criteria for the study were the following: patients aged over 60 years old; или patients’ age over 60 years old; the Injury Severity Score over 17, the presence of concomitant somatic pathology in patients. The exclusion criteria were the lack of complete necessary information in the medical history, patients’ age being less than 60 years old. The Geriatric Index of Comorbidity was calculated for each patient. A retrospective analysis was conducted.Results A Geriatric Index of Comorbidity of 3 or more in the elderly patients with polytrauma was found prognostically unfavorable for survival (p=0.005). When implementing the Geriatric Index of Comorbidity rating system, the presence of concomitant somatic pathology was found to have the greatest impact on elderly and senile patients with polytrauma and Injury Severity Score 18–24 (p=0.001).Conclusion The system of calculating the Geriatric Index of Comorbidity is advisable to use for assessing the risk of mortality from concomitant somatic pathology and its severity in patients with polytrauma over 60 years of age.