bluuebunny/crossref_metadata_2025_split huggingface dataset:
Restoration of hamstring function following sciatic nerve resection at the greater sciatic foramen with reconstruction involving acellular nerve allograft and vascularized sural nerve autograft: A case report
Authors: Sarcon Aida K. , Li Neill Y. , Houdek Matthew T. , Moran Steven L. 
Published: September 2022

AbstractSegmental loss of the sciatic nerve secondary to oncologic resection or trauma is detrimental to hamstring and leg function. The diameter of this nerve and the length of its axons spanning the lower extremity create significant challenges in reconstruction and optimizing return of sensory or motor function. The purpose of this report is to describe outcomes of a free vascularized sural nerve graft to preserve hamstring function in a large proximal sciatic nerve defect beginning at the greater sciatic foramen. A 44‐year‐old female underwent neoadjuvant chemotherapy and radiation for treatment of a left sciatic nerve synovial cell sarcoma. The patient underwent R0 resection of the proximal left sciatic nerve resulting in a 15 cm defect. An ipsilateral vascularized sural nerve graft was used to reconstruct the medial aspect of the sciatic nerve, prioritizing the tibial division, in an effort to restore hamstring function and plantar sensation. A 5 cm allograft nerve was added to the cutaneous branches of the sural nerve graft to better span the large defect and reconstruct the lateral aspect of the nerve. The patient's postoperative course was uneventful. At 1‐year follow‐up, the patient showed MRC grade 4/5 strength with knee flexion and steady gait pattern with a left ankle‐foot orthosis. Outcomes support the use of a single vascularized nerve graft alongside acellular nerve allograft to restore motor function in large diameter and large defect mixed nerve injuries.
Effect of a fall prevention strategy for the older patients: A quasi‐experimental study
Authors: Guo Xiaobei , Wang Ying , Wang Lei , Yang Xueke , Yang Weimei , Lu Zhihui , He Mei 
Published: September 2022

AbstractAimTo explore the effect of a fall prevention strategy on older patients based on the Patient Engagement Framework.DesignA longitudinal quasi‐experimental quantitative design.MethodsOlder patients who met the inclusion criteria were recruited from geriatric, oncology, neurology and cardiology departments of a teaching general hospital in China. Development of a fall prevention intervention strategy for older patients was based on the Patient Engagement Framework. Patients in the intervention group were given this fall prevention strategy (N = 58), and those in the control group were given conventional measures (N = 58). The following indicators were compared between the two groups after intervention:(a) number of falls; (b) Knowledge‐Attitude‐Practice (KAP) score;(c) Modified Fall Efficacy Scale score.ResultsAfter the implementation of an intervention strategy in older patients, the number of falls decreased from 3 to 0; the score of KAP and Modified Fall Efficacy Scale was promoted (p < .05).
Paradoxical perception of humanistic care in the intensive care unit: A qualitative study
Authors: Hasandoost Fateme , Mohammadi Eesa , Khademi Mojgan , Seddighi Mahyar 
Published: September 2022

AbstractAimThe aim of this study was to explore the perceptions of nurses, patients and attendants of humanistic care in the intensive care unit.DesignThe present study was a qualitative conventional content analysis conducted in the intensive care unit in a hospital in Iran, in 2019.MethodsData were collected using semi‐structured interviews and field notes through purposive sampling with 17 nurses who worked in an adult ICU in a teaching hospital, 4 attendants and 4 alert patients, and then analysed using the Elo‐Kyngäs method in 2008.ResultsAnalysis of the data led to the extraction of Four main themes as follows: (1) Insufficient understanding of nurses and patients' families of each other's roles, needs and expectations; (2) The use of personal and situational reasoning rather than ethical principles; (3) Caring stagnation; and (4) Satisfaction with care.
A comprehensive review of prognostic scoring systems to predict survival after transjugular intrahepatic portosystemic shunt placement
Authors: Yang Chongtu , Xiong Bin 
Published: September 2022

AbstractPatient prognosis after transjugular intrahepatic portosystemic shunt (TIPS) placement is relatively poor and highly heterogeneous; therefore, a prognostic scoring system is essential for survival prediction and risk stratification. Conventional scores include the Child–Turcotte–Pugh (CTP) and model for end‐stage liver disease (MELD) scores. The CTP score was created empirically and displayed a high correlation with post‐TIPS survival. However, the inclusion of subjective parameters and the use of discrete cut‐offs limit its utility. The advantages of the MELD score include its statistical validation and objective and readily available predictors that contribute to its broad application in clinical practice to predict post‐TIPS outcomes. In addition, multiple modifications of the MELD score, by incorporating additional predictors (e.g., MELD‐Sodium and MELD‐Sarcopenia scores), adjusting coefficients (recalibrated MELD score), or combined (MELD 3.0), have been proposed to improve the prognostic ability of the standard MELD score. Despite several updates to conventional scores, a prognostic score has been proposed (based on contemporary data) specifically for outcome prediction after TIPS placement. However, this novel score (the Freiburg index of post‐TIPS survival, FIPS) exhibited inconsistent discrimination in external validation studies, and its superiority over conventional scores remains undetermined. Additionally, several tools display potential for application in specific TIPS indications (e.g., bilirubin‐platelet grade for refractory ascites), and biomarkers of systemic inflammation, nutritional status, liver disease progression, and cardiac decompensation may provide additional value, but require further validation. Future studies should consider the effect of TIPS placement when exploring predictors, as TIPS is a pathophysiological approach that substantially alters systemic hemodynamics and ameliorates bacterial translocation and malnutrition.
Anisotropic Strange Star Model Beyond Standard Maximum Mass Limit by Gravitational Decoupling in f(Q)$f(Q)$ Gravity
Authors: Maurya S. K. , Singh Ksh. Newton , Lohakare Santosh V. , Mishra B. 
Published: September 2022

AbstractThe current theoretical development identified as the gravitational decoupling via Complete Geometric Deformation (CGD) method that has been introduced to explore the nonmetricity Q effects in relativistic astrophysics. In the present work, we have investigated the gravitationally decoupled anisotropic solutions for the strange stars in the framework of gravity by utilizing the CGD technique. To do this, we started with Tolman metric ansatz along with the MIT Bag model equation of state related to the hadronic matter. The solutions of the governing equations of motions are obtained by using two approaches, namely the mimicking of the θ sector to the seed radial pressure and energy density of the fluid model. The obtained models describe the self‐gravitating static, compact objects whose exterior solution can be given by the vacuum Schwarzschild Anti‐de Sitter spacetime. In particular, we modeled five stellar candidates, viz., LMC X‐4, PSR J1614‐2230, PSR J0740+6620, GW190814, and GW 170817 by using observational data. The rigorous viability tests of the solutions have been performed through regularity and stability conditions. We observed that the nonmetricity parameter and decoupling constant show a significant effect on stabilizing to ensure the physically realizable stellar models. The innovative feature of this work is to present the stable compact objects with masses beyond the without engaging of exotic matter. Therefore, the present study shows a new perception and physical significance about the exploration of ultra‐compact astrophysical objects.
RNAi‐mediated silencing of an egg‐specific gene Nllet1 results in hatch failure in the brown planthopper
Authors: Lu Jia‐Bao , Wang Sai‐Nan , Ren Peng‐Peng , He Fang , Li Qiao , Chen Jian‐Ping , Li Jun‐Min , Zhang Chuan‐Xi 
Published: September 2022

AbstractBackgroundThe brown planthopper (BPH) is one of the most destructive agricultural pests in Asia. RNA interference (RNAi)‐mediated pest management has been under development for years, and the selection of appropriate target genes is important for pest‐targeted RNAi. C‐type lectins (CTLs) are a class of genes that perform a variety of functions, such as the regulation of growth and development.ResultsA CTL‐S protein named Nllet1, containing a single calcium ion (Ca2+)‐dependent carbohydrate‐binding domain (CRD) with a conserved triplet motif QPD was identified and functionally characterized in BPH. Expression profiles at both the transcriptional and translational levels show that Nllet1 accumulates during the serosal cuticle (SC) formation period. Immunofluorescence and immunogold labeling further demonstrated that Nllet1 is located in the serosal endocuticle (en‐SC). Maternal RNAi‐mediated silencing of Nllet1 disrupted the SC structure, accompanied by a loss of the outward barrier and 100% embryo mortality. Injection of 10 ng dsNllet1 or dsNllet1' per female adult BPH resulted in a total failure of egg hatching.ConclusionNllet1 is essential for SC formation and embryonic development in BPH, which helps us understand the important roles of CTL‐Ss. Additionally, BPH eggs show high sensitivity to the depletion of Nllet1. This study indicates that Nllet1 is a promising candidate gene that can be used to develop RNAi‐based control strategies at the BPH egg stage, and it can also be used as a target for developing novel ovicides. © 2022 Society of Chemical Industry.
Sclareol attenuates angiotensin II‐induced cardiac remodeling and inflammation via inhibiting MAPK signaling
Authors: Yang Na , Zou Chunpeng , Luo Wu , Xu Diyun , Wang Mengyang , Wang Yi , Wu Gaojun , Shan Peiren , Liang Guang 
Published: September 2022

AbstractChronic inflammation plays an important role in hypertensive heart failure. Suppressing angiotensin II (Ang II)‐induced cardiac inflammation may contribute to the treatment of hypertension‐associated heart failure. Sclareol, a natural product initially isolated from the leaves and flowers of Salvia sclarea, possesses antiinflammatory and immune‐regulation activity in various systems. However, its effect on Ang II‐induced cardiac remodeling remains unknown. In this study, we have explored the potential effects of sclareol on Ang II‐induced heart failure. In vivo experiments were conducted in mice with Ang II‐pump infusion for 28 days. Sclareol administration at 5 mg·kg−1·d−1 significantly reduced the expression of myocardial injury markers. Sclareol also exerts protective effects against Ang II‐induced cardiac dysfunction in mice which is associated with alleviated cardiac inflammation and fibrosis. Transcriptome analysis revealed that inhibition of the Ang II‐activated mitogen‐activated protein kinase (MAPK) pathway contributed to the protective effect of sclareol. Sclareol inhibits Ang II‐activated MAPKs pathway to reduce inflammatory response in mouse hearts and cultured cardiomyocytes. Blockage of MAPKs in cardiomyocytes abolished the antiinflammatory effects of sclareol. In conclusion, we show that sclareol protects hearts against Ang II‐induced injuries through inhibiting MAPK‐mediated inflammation, indicating the potential use of sclareol in the prevention of hypertensive heart failure.
Hyperbaric oxygen rapidly improves tissue-specific insulin sensitivity and mitochondrial capacity in humans with type 2 diabetes: a randomised placebo-controlled crossover trial
Authors: Sarabhai Theresia , Mastrototaro Lucia , Kahl Sabine , Bönhof Gidon J. , Jonuscheit Marc , Bobrov Pavel , Katsuyama Hisayuki , Guthoff Rainer , Wolkersdorfer Martin , Herder Christian , Meuth Sven G. , Dreyer Sven , Roden Michael 
Published: September 2022

Abstract
Aims/hypothesis
Hyperbaric oxygen (HBO) therapy may improve hyperglycaemia in humans with type 2 diabetes, but underlying mechanisms are unclear. Our objective was to examine the glucometabolic effects of HBO on whole-body glucose disposal in humans with type 2 diabetes.

Methods
In a randomised placebo-controlled crossover trial located at the German Diabetes Center, 12 male individuals with type 2 diabetes (age 18–75 years, BMI <35 kg/m2, HbA1c 42–75 mmol/mol [6–9%]), randomly allocated by one person, underwent 2-h HBO, once with 100% (240 kPa; HBO) and once with 21% oxygen (240 kPa; control, CON). Insulin sensitivity was assessed by hyperinsulinaemic–euglycaemic clamps with d-[6,6-2H2]glucose, hepatic and skeletal muscle energy metabolism were assessed by 1H/31P-magnetic resonance spectroscopy, while high-resolution respirometry measured skeletal muscle and white adipose tissue (WAT) mitochondrial capacity. All participants and people assessing the outcomes were blinded.

Results
HBO decreased fasting blood glucose by 19% and increased whole-body, hepatic and WAT insulin sensitivity about one-third (p<0.05 vs CON). Upon HBO, hepatic γ-ATP concentrations doubled, mitochondrial respiratory control doubled in skeletal muscle and tripled in WAT (p<0.05 vs CON). HBO increased myocellular insulin-stimulated serine-473/threonine-308 phosphorylation of Akt but decreased basal inhibitory serine-1101 phosphorylation of IRS-1 and endoplasmic reticulum stress (p<0.05 vs CON).

Conclusions/interpretation
HBO-mediated improvement of insulin sensitivity likely results from decreased endoplasmic reticulum stress and increased mitochondrial capacity, possibly leading to low-dose reactive oxygen species-mediated mitohormesis in humans with type 2 diabetes.

Trial registration
ClinicalTrials.gov NCT04219215

Funding
German Federal Ministry of Health, German Federal Ministry of Education and Research, North-Rhine Westfalia Ministry of Culture and Science, European-Regional-Development-Fund, German-Research-Foundation (DFG), Schmutzler Stiftung

Graphical abstract
Epistasis-Driven Evolution of the SARS-CoV-2 Secondary Structure
Authors: Alemrajabi Mahsa , Macias Calix Ksenia , Assis Raquel 
Published: September 2022

AbstractEpistasis is an evolutionary phenomenon whereby the fitness effect of a mutation depends on the genetic background in which it arises. A key source of epistasis in an RNA molecule is its secondary structure, which contains functionally important topological motifs held together by hydrogen bonds between Watson–Crick (WC) base pairs. Here we study epistasis in the secondary structure of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by examining properties of derived alleles arising from substitution mutations at ancestral WC base-paired and unpaired (UP) sites in 15 conserved topological motifs across the genome. We uncover fewer derived alleles and lower derived allele frequencies at WC than at UP sites, supporting the hypothesis that modifications to the secondary structure are often deleterious. At WC sites, we also find lower derived allele frequencies for mutations that abolish base pairing than for those that yield G·U “wobbles,” illustrating that weak base pairing can partially preserve the integrity of the secondary structure. Last, we show that WC sites under the strongest epistatic constraint reside in a three-stemmed pseudoknot motif that plays an essential role in programmed ribosomal frameshifting, whereas those under the weakest epistatic constraint are located in 3’ UTR motifs that regulate viral replication and pathogenicity. Our findings demonstrate the importance of epistasis in the evolution of the SARS-CoV-2 secondary structure, as well as highlight putative structural and functional targets of different forms of natural selection.
Novel PCR detection of CRISPR/Cas systems in Pseudomonas aeruginosa and its correlation with antibiotic resistance
Authors: Soliman Mai , Said Heba Shehta , El-Mowafy Mohammed , Barwa Rasha 
Published: September 2022

Abstract

CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated proteins) systems are considered as acquired immune mechanisms in Gram-positive and Gram-negative bacteria and also in archaea. They provide resistance/immunity to attacking bacteriophages or mobile genetic elements as integrative conjugative elements (ICE) as well as plasmid transformation. As an opportunistic pathogen, Pseudomonas aeruginosa has been held responsible for serious infections especially in hospitalized and immunocompromised patients. Three subtypes of type I CRISPR system (I-C, I-E, & I-F1) have been detected in P. aeruginosa genomes. In this work, P. aeruginosa isolates were collected from different clinical sources, and the three CRISPR/Cas subtypes (I-C, I-E, & I-F1) were detected via singleplex and multiplex PCR techniques using novel universal primers that were designed specifically in this study. CRISPR subtypes I-C, I-E, and I-F1 were detected in 10, 9, and 13 isolates, respectively. Furthermore, antimicrobial susceptibility of CRISPR/Cas-positive and negative isolates to different antibiotics and the capacity of biofilm formation were detected using disc diffusion method and tissue culture plate method, respectively. There was a significant correlation between the presence/absence of CRISPR/Cas system and both antimicrobial susceptibility to some antibiotics and biofilm-forming capacity among P. aeruginosa clinical isolates.

Key points
• A novel multiplex–PCR for detection of CRISPR/Cas-positive strains of P. aeruginosa.
• Understand the correlation between CRISPR/Cas systems and other characters of P. aeruginosa.
• Correlation between antimicrobial susceptibility and CRISPR systems in P. aeruginosa.
Structural brain changes in patients with persistent headache after COVID-19 resolution
Authors: Planchuelo-Gómez Álvaro , García-Azorín David , Guerrero Ángel L. , Rodríguez Margarita , Aja-Fernández Santiago , de Luis-García Rodrigo 
Published: September 2022

AbstractHeadache is among the most frequently reported symptoms after resolution of COVID-19. We assessed structural brain changes using T1- and diffusion-weighted MRI processed data from 167 subjects: 40 patients who recovered from COVID-19 but suffered from persistent headache without prior history of headache (COV), 41 healthy controls, 43 patients with episodic migraine and 43 patients with chronic migraine. To evaluate gray matter and white matter changes, morphometry parameters and diffusion tensor imaging-based measures were employed, respectively. COV patients showed significant lower cortical gray matter volume and cortical thickness than healthy subjects (p < 0.05, false discovery rate corrected) in the inferior frontal and the fusiform cortex. Lower fractional anisotropy and higher radial diffusivity (p < 0.05, family-wise error corrected) were observed in COV patients compared to controls, mainly in the corpus callosum and left hemisphere. COV patients showed higher cortical volume and thickness than migraine patients in the cingulate and frontal gyri, paracentral lobule and superior temporal sulcus, lower volume in subcortical regions and lower curvature in the precuneus and cuneus. Lower diffusion metric values in COV patients compared to migraine were identified prominently in the right hemisphere. COV patients present diverse changes in the white matter and gray matter structure. White matter changes seem to be associated with impairment of fiber bundles. Besides, the gray matter changes and other white matter modifications such as axonal integrity loss seemed subtle and less pronounced than those detected in migraine, showing that persistent headache after COVID-19 resolution could be an intermediate state between normality and migraine.
A longitudinal analysis of nosocomial bloodstream infections among preterm neonates
Authors: Jansen Sophie J. , van der Hoeven Alieke , van den Akker Thomas , Veenhof Marieke , von Asmuth Erik G. J. , Veldkamp Karin Ellen , Rijken Monique , van der Beek Martha , Bekker Vincent , Lopriore Enrico 
Published: September 2022

Abstract
Nosocomial bloodstream infections (NBSIs), commonly due to central-line associated bloodstream infections (CLABSI), contribute substantially to neonatal morbidity and mortality. We aimed to identify longitudinal changes in incidence of NBSI, microbiological-spectrum, and antibiotic exposure in a large cohort of preterm neonates admitted to the neonatal intensive care unit. We retrospectively assessed differences in annual rates of NBSI (per 1000 patient-days), CLABSI (per 1000 central-line days), and antibiotic consumption (per 1000 patient-days) among preterm neonates (< 32 weeks’ gestation) hospitalized between January 2012 and December 2020. Multi-state Markov models were created to model states of progression of NBSI and infection risk given a central-line on days 0, 3, 7, and 10 of admission. Of 1547 preterm infants, 292 (19%) neonates acquired 310 NBSI episodes, 99 (32%) of which were attributed to a central-line. Over the years, a significant reduction in central-line use was observed (p < 0.001), although median dwell-time increased (p = 0.002). CLABSI incidence varied from 8.83 to 25.3 per 1000 central-line days, with no significant difference between years (p = 0.27). Coagulase-negative staphylococci accounted for 66% of infections. A significant decrease was found in antibiotic consumption (p < 0.001). Probability of NBSI decreased from 16% on day 3 to 6% on day 10. NBSI remains a common problem in preterm neonates. Overall antibiotic consumption decreased over time despite the absence of a significant reduction in infection rates. Further research aimed at reducing NBSI, in particular CLABSI, is warranted, particularly with regard to limiting central-line dwell-time and fine-tuning insertion and maintenance practices.
Nomogram based on MRI can preoperatively predict brain invasion in meningioma
Authors: Zhang Jing , Cao Yuntai , Zhang Guojin , Zhao Zhiyong , Sun Jianqing , Li Wenyi , Ren Jialiang , Han Tao , Zhou Junlin , Chen Kuntao 
Published: September 2022

AbstractPredicting brain invasion preoperatively should help to guide surgical decision-making and aid the prediction of meningioma grading and prognosis. However, only a few imaging features have been identified to aid prediction. This study aimed to develop and validate an MRI-based nomogram to predict brain invasion by meningioma. In this retrospective study, 658 patients were examined via routine MRI before undergoing surgery and were diagnosed with meningioma by histopathology. Least absolute shrinkage and selection operator (LASSO) regularization was used to determine the optimal combination of clinical characteristics and MRI features for predicting brain invasion by meningiomas. Logistic regression and receiver operating characteristic (ROC) curve analyses were used to determine the discriminatory ability. Furthermore, a nomogram was constructed using the optimal MRI features, and decision curve analysis was used to validate the clinical usefulness of the nomogram. Eighty-one patients with brain invasion and 577 patients without invasion were enrolled. According to LASSO regularization, tumour shape, tumour boundary, peritumoral oedema, and maximum diameter were independent predictors of brain invasion. The model showed good discriminatory ability for predicting brain invasion in meningiomas, with an AUC of 0.905 (95% CI, 0.871–0.940) vs 0.898 (95% CI, 0.849–0.947) and sensitivity of 93.0% vs 92.6% in the training vs validation cohorts. Our predictive model based on MRI features showed good performance and high sensitivity for predicting the risk of brain invasion in meningiomas and can be applied in the clinical setting.