bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceptions of gender equity and markers of achievement in a National Institute for Health Research (NIHR) Biomedical Research Centre (BRC): A qualitative study.
Authors: Henderson Lorna R , Dam Rinita , Shah Syed Ghulam Sarwar , Ovseiko Pavel V , Kiparoglou Vasiliki 
Published: May 2022

Abstract
Background The need to improve gender equity (GE) in academic medicine is well documented. Biomedical Research Centres (BRCs), partnerships between leading National Health Service (NHS) organisations and universities in England, conduct world-class translational research funded by the National Institute for Health Research (NIHR). In 2011, eligibility for BRC funding was restricted to universities demonstrating sustained GEsuccess recognised by the Athena SWAN Charter for Women in Science Silver awards. Despite this structural change, GE research in BRC settings is underdeveloped, yet critical to the acceleration of women’s advancement and leadership.Objectives To explore both women’s and men’s perceptions of GE and current markers of achievement in a BRC setting.Methods Thematic analysis of data from two discrete research projects: 53 GE survey respondents’ free text comments (34 women, 16 men), and 16 semi structured interviews with women affiliated to the NIHR Oxford BRC.Results: Four major themes emerged from the analysis: perceptions of the Athena Swan Charter for Women in Science (GE policy); views on monitoring GE in BRCs; views on current markers of achievement in academia and GE; and recommendations for actions to improve GE in BRC settings. Monitoring of GE in BRCs was deemed to be important, but complex. Participants felt current markers of achievement were not equitable to women as theydid not take contextual factors into account such as maternity leave and caring responsibilities. BRC specific organisational policies and metrics are required to monitor and catalyseGE.Conclusions Markers of achievement for monitoring GE in BRCs should take intoaccount contextual factors specific to BRCs and women’s career progression and professional advancement. GE markers of achievement should be complimented with broader aspects of equality, diversity and inclusion.
Examination of the Psychometric Properties of Pediatric-Modified Total Neuropathy Score in Turkish Children with Cancer
Authors: Özdemir Bilge , Gerçeker Gülçin Özalp , Özdemir Emine Zahide , Yıldırım Büşra Güliz , Ören Hale , Yiş Uluç , Günay Çağatay , Thomas Gülten Öztürk 
Published: May 2022

Abstract
Background and Aims: Evaluation of chemotherapy-induced peripheral neuropathy has gained importance in symptom management of pediatric patients with cancer. The aim of this study is to perform the Turkish validity and reliability study of the Pediatric-Modified Total Neuropathy Score (Ped-mTNS).Methods: A methodological, descriptive, and cross-sectional design was used in the study. Forty children who were aged between 5 and 18 and were treated for cancer and 40 age- and gender-matched healthy children (control group) were included in the study. The mean scores of the items on the Ped-mTNS were compared, and item-total score correlations were evaluated. Cronbach’s alpha coefficient of the Ped-mTNS was calculated for internal consistency.Results: Cronbach’s alpha value of the scale was found as .709 in the study. The item-total correlations of the scale items ranged from 0.260 to 0.658. The mean score of cancer patients on the Ped-mTNS was found as 4.4±3.8. The Ped-mTNS scores of children with cancer indicated more deficits than those of the control group. In the evaluation of children in the patient and control groups, a difference was found in terms of light touch sensation, which is one of the sensory symptoms in the items of the Ped-mTNS, and pin sensibility and strength, which are among clinical symptoms. The Ped-mTNS was determined to be a valid and reliable measurement tool for children with cancer who are aged between 5 and 18 in the Turkish population.
High Percentage of Cancer Stem Cells in Metastatic Locations: Upregulation of circBIRC6 in Highly Metastatic Breast Cancer Subline
Authors: Kamalabadi-Farahani Mohammad , Karimi Roqaye , Atashi Amir 
Published: May 2022

Abstract
Metastasis is a devastating complication in breast cancer. Cancer relapse and metastasis are associated with cancer stem cells. CircBIRC6 is a circular RNA that proposed to be involved in stemness of stem cells. In breast cancer, metastatic tumor cells have higher stem cell properties. In the present study we evaluate the expression of circBIRC6 in these cells. After development of syngenic animal model of TNBC, primary breast cancer cells named 4T1T were isolated from tumor mass. Highly metastatic tumor cells named 4T1B and 4T1L were isolated and expanded from brain metastasis lesions and lung of cancerous mice respectively. Sphere formation ability in metastatic and primary tumor cells was evaluated separately. Quantitative real-time polymerase chain reaction was performed to analyze the expression of circBIRC6 in primary and metastatic tumor cells. Our data revealed that, sphere formation ability among metastatic tumor cells was significantly higher. Surprisingly expression of circBIRC6 was significantly upregulated in these metastatic tumor cells. In comparison with 4T1T, circBIRC6 was upregulated 5.7 and 3.5 time in 4T1B and 4T1L respectively. These findings provided important insights regarding the molecular properties of metastatic tumor cells and can be used to design targeted therapeutic strategies to combat these cells.
The underlying mechanism of Guillain-Barré syndrome in a young patient suffered from Japanese encephalitis virus infection: a case report
Authors: Liu Sheng , Wang Jinyong , Yang Jun , Wen Ying 
Published: May 2022

Abstract
Background: The presentation of Guillain-Barré syndrome (GBS) suffered from Japanese encephalitis virus (JEV) is uncommonthoughthe clusters of GBS cases were observed in Chinain 2018. The underlying mechanism was not clarified especially in individuals with JE vaccination in childhood. Case presentation: Here we reported a patient suffered from acute flaccid paralysis (AFP) involvement with four extremities and respiratory muscles, while showed normal images by brain and spine magnetic resonanceimaging (MRI). Electrophysiology examination displayed slowed conduction speed of motor nerves and declined amplitude of evoked velocity. GBS was finally considered related to JEV verified by both positive anti-JEV immunoglobulin M antibody and positive immunoglobulin G antibody in serum. Unfortunately, he died after declining mechanical ventilator and intravenous immunoglobulin at 36th day of illness onset. We also made a review of related cases reported previously and discussed the underlying mechanism.Conclusion: JEV infection-associated GBS was an unusual clinical appearance. We should pay attention to the atypical manifestations of JEV infection and explore possible pathogenesis behind particular individuals.
Hexokinase 2 Promoted Cell Motility and Proliferation by Activating Akt1/p- Akt1 in Human Ovarian Cancer Cells
Authors: Tian Xueye , Liu Dan , Zuo Xiaohang , Sun Xiaoli , Wu Mengmin , Li Xu , Teng Yue 
Published: May 2022

Abstract
Background: Recently, increasing evidence has indicated that elevation of Hexokinase 2 (HK2) plays an important role in several cancers on regulating cell motility and growth. However, its role on regulating cell EMT in human ovarian cancer still less to known.Methods: The transwell and wound-healing assay were used to detect the effective of HK2 on regulating motility of ovarian cancer cells. Real Time PCR and Western Blotting were used to explore the changing of EMT-related proteins in HK2-modified cells. The clonogenic formation, cell growth curves and MTT assays were used to evaluate the effective of HK2 on regulating cell proliferation in HK2-modified cells. The flow cytometry was used to detect the differences in the distribution of cells in the cell cycle between the HK2-modified cells and their control cells. The correlation of HK2 and Akt1/p-Akt1 was explored by using Western Blotting, Akt1 inhibitor (MK2206) and transient transfection of an Akt1 recombinant plasmid. The potential correlation between HK2 and EMT-related proteins in human ovarian cancer tissues and OV (ovarian serous cystadenocarcinoma) was confirmed by using Pearson correlation analysis and TIMER 2.0.Results: In ovarian cancer cells, overexpressing of HK2 enhanced cell motility by inducing of EMT-related proteins, such as CDH2, fibronectin, MMP9, ZEB1, ZEB2 and vimentin. Moreover, overexpressing of HK2 promoted cell growth by reducing p21 and p27 expression in ovarian cancer cells. Further studies demonstrated that this promotion of cell motility and growth by HK2 was probably a result of it activating of Akt1 (p-Akt1) in ovarian cancer cells. Additionally, the positive correlation between HK2 and p-Akt1, fibronectin, MMP9 expression in human ovarian cancer samples was verified by using Pearson correlation analysis. The positive correlation between HK2 and CDH2, fibronectin, MMP9, ZEB1, ZEB2 and vimentin in OV (ovarian serous cystadenocarcinoma) was confirmed by using TIMER 2.0.Conclusion: This study demonstrated that HK2 could induce EMT-related proteins and reduce cell cycle inhibitor by activating Akt1 in human ovarian cancer cells, subsequently enhancing cell motility and growth, suggesting that HK2 participate in the malignant process of ovarian cancer by interacting with Akt1.
Sjögren’s Syndrome with Bipolar Disorder, A Case Report
Authors: Khalayli Naram , Kudsi Maysoun 
Published: May 2022

Abstract
Sjögren’s syndrome is an autoimmune disease that affects multiple organs manifesting as different symptoms and signs. Neuropsychiatric manifestations are one of the symptoms and are common at the onset of the disease and during its course. The most frequent symptoms are depression and anxiety (5). However, the association with bipolar disorder seems rare and not frequently documented. Our case report presents a Sjögren’s syndrome patient with bipolar disorder, suggesting that this could lead to delayed diagnosis and treatment. If it is suspected, a better analysis should be done by psychiatrists to help early diagnosis and adapting prescription. Corticosteroid treatment used in Sjögren’s syndrome should be avoided and, if prescribed, used with caution in bipolar disorder.
Integrative Analysis of Transcriptome-Wide Association Study and Gene-Based Association Analysis Identifies Candidate Genes Associated With Juvenile Idiopathic Arthritis
Authors: Liu Shuai , Gong Weiming , Liu Lu , Yan Ran , Wang Shukang , Yuan Zhongshang 
Published: May 2022

Abstract
BackgroundThe current genome-wide association study (GWAS) of Juvenile idiopathic arthritis (JIA) suffers from the low power due to limited sample size, as well as suffers from the interpretation issue due to most GWAS signals located in non-coding regions. Gene-level analysis is able to aggregate many SNPs with small effects to improve the association power, and further benefit the better understanding of genetic determinants underlying JIA. MethodsUsing the largest GWAS summary statistics of JIA to date (3305 cases and 9196 controls), we performed transcriptome-wide association studies (TWAS) using FUSION, parallelized with gene-based association analysis using the eMAGMA method, to identify the potential tissue-specific genes related with JIA. We then explore the novel JIA-associated genes through overlapping the genes significantly detected from these two typical gene-level analyses to avoid the risk of false discoveries from using single method, followed by enrichment analysis to identify the significant gene ontology terms as well as the pathways. ResultsA total of 33 unique genes had been significantly identified from both TWAS analysis and eMAGMA gene-based association analysis, of which 11 were previously reported, including TYK2 (PFUSION = 5.12 × 10-6, PeMAGMA = 1.94 × 10-7 for Whole Blood), IL(Interleukin)-6R (PFUSION = 8.63 × 10-7, PeMAGMA = 2.74 × 10-6 for Cells EBV-transformed lymphocytes) and Fas (PFUSION = 5.21 × 10-5, PeMAGMA = 1.08 × 10-6 for Muscle Skeletal). There are totally 22 newly reported genes indicating the power advantage of gene-level association analysis, of which some are more likely JIA-associated genes, including IL-27 (PFUSION = 2.10 × 10-7, PeMAGMA = 3.93 × 10-8 for Liver), LAT (PFUSION = 1.53 × 10-4, PeMAGMA = 4.62 × 10-7 for Artery Aorta) and MAGI3 (PFUSION = 1.30 × 10-5, PeMAGMA = 1.73 × 10-7 for Muscle Skeletal). Enrichment analysis of 33 common genes further implicated the significant roles of 10 GO terms as well as 4 KEGG pathways including Th17 cell differentiation (P=5.83×10-6) and Rap1 signaling pathway (P=1.55×10-3). ConclusionsOur findings provide novel insights into the genetic determinants of JIA, which could benefit the understanding of pathogenic mechanisms as well as potential therapeutic targets of JIA.
The Effect of Blood Flow Restriction Training Combined with Electrical Muscle Stimulation on Neuromuscular Adaptation: A Randomized Controlled Trial
Authors: Li Na , Yang Jingfeng , Jiang Ning , Liao Yuanpeng 
Published: May 2022

Abstract
Background: For postoperative rehabilitation patients, athletes with skeletal muscle injury, the elderly and other populations with special needs, high-load and high-intensity resistance training is not practical. Low-intensity resistance training (≤25% 1RM) combined with blood flow restriction training (BFRT) is beneficial to increasing muscle mass and muscle strength, but it cannot produce increased muscle activation and neuromuscular adaptation, as traditional high-intensity strength training does. Therefore, the purpose of this study is to investigate the effects of independently applying BFRT and electrical muscle stimulation (EMS), as well as combining the two methods, on muscle function. Methods: Forty healthy participants with irregular exercise experiences were randomly assigned to four groups: BFRT-alone group (BFRT, n=10), EMS-alone group (EMS, n=10), BFRT combined with EMS group (CMB, n=10), and the control group (CTR, n=10). All participants received low-intensity squat training at a load of 25% 1RM 5 times/week for 6 weeks. Cross-sectional area (CSA) and electromyographic root mean square (RMS) in the rectus femoris, as well as peak torque (PT) of the knee extensor were measured prior to and following a 6-week intervention. Results: Following the 6-week intervention, the increases in muscle activation in the CMB group were statistically higher than those in the BFRT group (p < 0.001), but not different from those in the EMS group (p = 0.986). The combined stimulation technique can induce muscle activation levels similar to that of the EMS-alone group, thus compensating for the lack of muscle activation when BFRT is used alone. Conclusion: The combination of BFRT and EMS for low-intensity squat training improved the muscle strength of the lower limbs by promoting muscle hypertrophy and improving the muscle activation, likely because such a combination compensates for the limitations and deficiencies of the two intervention methods when applied alone.
Changes in the Bacillus pumilus proteome during 2,4,6-trinitrotoluene degradation
Authors: Yakovleva Galina , Kurdy William , Gorbunova Anna , Khilyas Irina , Lochnit Guenter , Ilinskaya Olga 
Published: May 2022

Abstract
2,4,6-Trinitrotoluene (TNT) is the most widely used nitroaromatic compound and is highly resistant to degradation. Most aerobic microorganisms reduce TNT to amino derivatives via formation of nitroso- and hydroxylamine intermediates. Although pathways of TNT degradation are well studied, proteomic analysis of TNT-degrading bacteria was done only for some individual Gram-negative strains. Here, we isolated a Gram-positive strain from TNT-contaminated soil, identified it as Bacillus pumilus using 16S rRNA sequencing, analyzed its growth, the level of TNT transformation, ROS production, and revealed for the first time the bacillary proteome changes at toxic concentration of TNT. The transformation of TNT at all studied concentrations (20–200 mg/L) followed the path of nitro groups reduction with the formation of 4-amino-2,6-dinitrotoluene. Hydrogen peroxide production was detected during TNT transformation. Comparative proteomic analysis of B. pumilus showed that TNT (200 mg/L) inhibited expression of 46 and induced expression of 24 proteins. Among TNT upregulated proteins are those which are responsible for the reductive pathway of xenobiotic transformation, removal of oxidative stress, DNA repair, degradation of RNA and cellular proteins. The production of ribosomal proteins, some important metabolic proteins and proteins involved in cell division are downregulated by this xenobiotic.
Cooperativity of IGF2BP1 and ETV6-RUNX1 in the pathogenesis of ETV6- RUNX1 positive B-Cell Acute Lymphoblastic Leukemia
Authors: Sharma Gunjan , Tran Tiffany M , Bassi Jaspal , Tan Yuande , Jaiswal Amit Kumar , Tso Christine , Jain Ayushi , Singh Jay , Chopra Anita , Singh Archna , Bakhshi Sameer , Chattopadhyay Parthaprasad , Casero David , Rao Dinesh Subba , Palanichamy Jayanth Kumar 
Published: May 2022

Abstract
Background
ETV6-RUNX1 is the most prevalent translocation in pediatric B-ALL (B-cell Acute Lymphoblastic Leukemia). However, the exact pathogenesis of this translocation leading to leukemogenesis remains unclear. Insulin like Growth Factor 2 Binding Protein 1 (IGF2BP1), an oncofetal RNA binding protein (RBP), is overexpressed in ETV6-RUNX1 positive B-ALL. We intended to investigate the role of IGF2BP1 in leukemogenesis in this study.
Methods
qRT-PCR was used to analyze IGF2BP1 overexpression in an Indian cohort of pediatric B-ALL (n = 167). IGF2BP1 and ETV6-RUNX1 were knocked out in Reh, an ETV6-RUNX1 positive B-ALL cell line, using CRISPR/Cas9 technology. Cell proliferation assays were used to study cell survival after IGF2BP1/ETV6-RUNX1 KO and prednisolone response. RNA-Sequencing after IGF2BP1 knockout and RNA Immunoprecipitation sequencing (RIP-Seq) after IGF2BP1 pulldown were performed to find the putative targets of IGF2BP1. Luciferase reporter assays were used to study downstream mRNA target stability as well as pathways regulated by IGF2BP1. Finally, to study the in-vivo synergistic effect between ETV6-RUNX1 fusion protein and IGF2BP1, a murine bone marrow transplant model was utilized followed by flowcytometric analysis of hematopoietic tissues and histopathological examination.
Results
IGF2BP1 expression was found to be essential for tumor cell survival in Reh cell line. Knockout of IGF2BP1 or ETV6-RUNX1 led to reduced proliferation and increased sensitivity to prednisolone hinting at synergism between both the genes. Integrated analysis of transcriptome sequencing after IGF2BP1 knockout and RIP-Seq revealed that IGF2BP1 targets encompass multiple pro-oncogenic signalling pathways including TNFα/NFκB and PI3K-Akt pathways. Dysregulation of these pathways was also observed in patient samples. Mechanistically, we demonstrate that IGF2BP1 binds and stabilizes ETV6-RUNX1 fusion transcript and the presence of a positive feedback loop between them which maintains a constitutive dysregulation of several oncogenic pathways.
Study on safe usage of agricultural land in typical karst areas based on Cd in soil and maize: A case study of northwestern Guizhou, China
Authors: Yang Liyu , Wu Pan , Yang Wentao , Xu Mengqi , Chen Yonglin , Jing Haonan , Wang Ping 
Published: May 2022

Abstract
Cadmium (Cd) is vital dietary toxins that are harmful to human health. The Cd translocation in soil-crops system varies greatly depending on different soil matrices; therefore, a valuable method that could accurately evaluate soil Cd thresholds needs to be proposed immediately. In the southwestern part of China, a typical karst mountainous area of east Asia, the results of our survey of 492 soil-maize samples in the region showed high Cd accumulation in soil, with concentrations ranging from 0.07 to 31.95 mg kg − 1. The Cd concentrations in maize kernels planted in those fields, however, were quite low, and only 4 samples exceeded the national standard. A comparative study with non-karst areas revealed a low bioaccumulation factor for soil Cd. This may be interpreted as the weathering soil-forming process of mineral-bearing rock systems, leading to high accumulation as well as low bioavailability of Cd in karst soils. A total of 172 soil-maize samples were evaluated inaccurately by the national standard evaluation procedure, accounting for 34.96% of the total. Therefore, we proposed the species sensitivity distribution model to address this inaccurate assessment. The results show that the hazardous concentrations of 95% and 5% in maize fields were 2.2 and 85.1 mg/kg for soil pH ≤ 5.5, 2.5 and 108.5 mg/kg for 5.5 < pH ≤ 6.5, 3.0 and 161.8 mg/kg for 6.5 < pH ≤ 7.5, respectively. The total number of unsuitable samples according to the evaluation results decreased from 172 to 2 after modification. Therefore, this result could be considered as a more accurate assessment threshold.
Prerequisites for coexistence: human pressure and refuge habitat availability shape continental-scale habitat use patterns of a large carnivore
Authors: Oeser Julian , Heurich Marco , Kramer-Schadt Stephanie , Andrén Henrik , Bagrade Guna , Belotti Elisa , Bufka Luděk , Breitenmoser-Würsten Christine , Černe Rok , Duľa Martin , Fuxjäger Christian , Gomerčić Tomislav , Jędrzejewski Włodzimierz , Kont Raido , Koubek Petr , Kowalczyk Rafał , Krofel Miha , Krojerová-Prokešová Jarmila , Kubala Jakub , Kusak Josip , Kutal Miroslav , Linnell John D.C. , Mattisson Jenny , Molinari-Jobin Anja , Männil Peep , Odden John , Okarma Henryk , Oliveira Teresa , Pagon Nives , Persson Jens , Remm Jaanus , Schmidt Krzysztof , Signer Sven , Tám Branislav , Vogt Kristina , Zimmermann Fridolin , Kuemmerle Tobias 
Published: May 2022

Abstract
ContextBehavioral adjustments by large carnivores can be a key factor facilitating their coexistence with people in shared landscapes. Landscape composition might be a key factor determining how large carnivores can adapt to occurring alongside humans, yet broad-scale analyses investigating adjustments of habitat use across large gradients of human pressure and landscape composition are lacking.ObjectivesHere, we investigate adjustments in habitat use by Eurasian lynx (Lynx lynx) in response to varying availability of refuge habitats (i.e., forests and rugged terrain) and human landscape modification.MethodsWe used a large tracking dataset including 434 individuals from seven populations to analyze variation in lynx’ use of refuge habitats and landscape modification at a continental scale.ResultsWe found that lynx use refuge habitats more intensively with increasing landscape modification, selecting forests most strongly in otherwise open landscapes and rugged terrain in mountainous regions. Higher forest availability enabled lynx to place their home ranges in more human-modified landscapes. Human pressure and refuge habitat availability also shaped temporal patterns of lynx habitat use, affecting daytime-nighttime differences in refuge habitat use, as well as females’ association with human-modified habitats during the first months after kittens are born.ConclusionsOur findings suggest a remarkable adaptive capacity of lynx towards human pressure and underline the importance of refuge habitats for enabling coexistence between large carnivores and people. More broadly, we highlight that the composition of landscapes determines how large carnivores can adapt to human pressure, and that both factors interact in shaping large carnivore habitat use and distributions at broad scales.