bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel case of cutaneous myxoid spindle cell neoplasm with FMR1‐ALK gene fusion and CD34/S100 co‐expression
Authors: Kasago Israel , Aypar Umut , Sukhadia Purvil , Vanderbilt Chad , Ladanyi Marc , Hurd Tierra , Pulitzer Melissa 
Published: November 2022

AbstractA novel class of superficial CD34+ and S100+ cutaneous spindle cell neoplasm harboring ALK rearrangements has recently been described. Morphologically, these neoplasms have been characterized by bland spindled cells organized in whorls and cords against myxoid stroma, eventuating in the designation “superficial ALK‐rearranged myxoid spindle cell neoplasm.” Here, we report a 78‐year‐old male with a 3‐mm pink papule on the chest, clinically concerning for cutaneous carcinoma. Biopsy of the specimen showed a biphasic tumor with hypercellular and hypocellular zones consisting of epithelioid cells and monomorphic, bland spindled cells. The spindled cells were arranged in perineurial‐like concentric whorls and cords embedded in a myxo‐collagenous stroma. Neoplastic cells were diffusely positive for CD34, S100, and D5F3‐ALK, without SOX10 expression. Negative markers included GLUT1, EMA, factor XIIIa, desmin, actin, and SMA. ALK‐rearrangement was identified on fluorescence in situ hybridization break‐apart assay. A corresponding novel FMR1‐ALK fusion was found by next‐generation sequencing (NGS) based RNA sequencing. Identification of this new FMR1‐ALK fusion signature adds to the spectrum of diagnostic genomic alterations in this newly described class of tumors.
Oleic acid‐bound FABP7 drives glioma cell proliferation through regulation of nuclear lipid droplet formation
Authors: Umaru Banlanjo Abdulaziz , Kagawa Yoshiteru , Ohsaki Yuki , Pan Yijun , Chen Chuck T. , Chen Daniel K. , Abe Toshiaki , Shil Subrata Kumar , Miyazaki Hirofumi , Kobayashi Shuhei , Maekawa Motoko , Yamamoto Yui , Wannakul Tunyanat , Yang Shuhan , Bazinet Richard P. , Owada Yuji 
Published: November 2022

Fatty acid‐binding protein 7 (FABP7), one of the fatty acid (FA) chaperones involved in the modulation of intracellular FA metabolism, is highly expressed in glioblastoma, and its expression is associated with decreased patients' prognosis. Previously, we demonstrated that FABP7 requires its binding partner to exert its function and that a mutation in the FA‐binding site of FABP7 affects tumour biology. Here, we explored the role of FA ligand binding for FABP7 function in tumour proliferation and examined the mechanism of FABP7 and ligand interaction in tumour biology. We discovered that among several FA treatment, oleic acid (OA) boosted cell proliferation of FABP7‐expressing cells. In turn, OA increased FABP7 nuclear localization, and the accumulation of FABP7–OA complex in the nucleus induced the formation of nuclear lipid droplet (nLD), as well as an increase in colocalization of nLD with promyelocytic leukaemia (PML) nuclear bodies. Furthermore, OA increased mRNA levels of proliferation‐related genes in FABP7‐expressing cells through histone acetylation. Interestingly, these OA‐boosted functions were abrogated in FABP7‐knockout cells and mutant FABP7‐overexpressing cells. Thus, our findings suggest that FABP7–OA intracellular interaction may modulate nLD formation and the epigenetic status thereby enhancing transcription of proliferation‐regulating genes, ultimately driving tumour cell proliferation.
An integrated scheme for the management of drifting fish aggregating devices in tuna purse seine fisheries
Authors: Song Liming , Shen Huihui 
Published: November 2022

AbstractDrifting fish aggregating devices (dFADs) are extensively used in modern tuna purse seine fisheries. The use of dFADs has greatly improved fishing efficiency and generated substantial economic returns in purse seine fisheries, but adverse impacts on tuna juveniles, associated species and marine ecosystems are still of concern. Based on an analysis of current challenges in dFAD management measures in tuna regional fishery management organisations (t‐RFMOs), we explored opportunities to strengthen dFAD management in tuna fisheries. An integrated dFAD management scheme with multiple tools that account for different usage stages of a dFAD needs to be organised to regulate, track, monitor and recover in tuna purse seine fisheries. We recommend that (1) dFAD‐associated definitions should be standardised to provide operational guidance for dFAD activities; (2) all deployed dFADs should be counted towards dFAD limits on active dFADs, except those reported as lost and abandoned; (3) a regional dFAD tracking and monitoring program should track dFAD paths based on daily position reported to RFMOs directly, in addition to mandatory dFAD marking requirements; and (4) a framework of compensation mechanisms should provide financial support for joint dFAD recovery and eco‐compensation.
Cryptic diversity of Oxythyrea flower chafers and its implication for conservation of non‐forest biotopes in the Balkans
Authors: Vondráček Dominik , Král David , Šípek Petr 
Published: November 2022

Abstract
Flower chafers (Coleoptera: Scarabaeidae: Cetoniinae) are usually associated with saproxylophagous life strategies, and several of these taxa are considered as flagship species of insect conservation in Europe.
Here, we discuss the significance of soil‐dwelling flower chafers for the conservation of natural habitats by reporting the rediscovery of two relict, habitat specialist flower chafers of the genus Oxythyrea in Europe.
We compare the observed population structure of both species with their widespread congeners and discuss the detected deep genetic divergences among populations of both species, O. dulcis and O. albopicta.
Surprisingly, deep genetic structure has been observed among the psammophilous O. dulcis, which is quite counterintuitive as psammophilous habitats are rather ephemeral and these species tend to be very vagile.
Populations of soil‐dwelling, habitat specialist flower beetles may represent relicts of the past paleogeographic events, and their conservation value has to be thoroughly assessed.
Ataxia telangiectasia mutated kinase inhibition promotes irradiation‐induced PD‐L1 expression in tumour‐associated macrophages through IFN‐I/JAK signalling pathway
Authors: Gao Yuke , Li Yangyi , Lin Zaihuan , Zeng Yuxin , Huang Zhengrong , Han Linzhi , Zhong Yahua , Gong Yan , Wu Qiuji , Xie Conghua 
Published: November 2022

AbstractTumour‐associated macrophages (TAMs) are one of the primary sources of PD‐L1 expression in the tumour microenvironment (TME). Ionizing radiation (IR) promotes PD‐L1 expression in tumour cells. However, the effect of IR on macrophage PD‐L1 expression and the underlying mechanisms remain unclear. ATM kinase, as the key kinase for initiating DNA damage repair (DDR) process, is associated with innate immune STING axis activation. Here, we explored the molecular mechanism implicated in macrophage PD‐L1 expression regulated by IR as well as the role of ATM kinase in this process. IR‐regulated PD‐L1 expression in macrophages and associated signalling pathways were explored by in vitro studies using murine and human macrophage cell lines. A colorectal xenograft murine model was employed to demonstrate the impact of targeting ATM and PD‐L1 expression in TAMs following IR on growth of tumour in vivo. IR up‐regulated PD‐L1 expression in macrophages, which was further augmented by ATM kinase inhibition. ATM inhibition increased IR‐induced DNA damage, which activated STING/interferon regulatory factor 3 (IRF3) signalling pathway and up‐regulated type I interferon (IFN‐I) expression in macrophages. IFN‐I bound to the IFN α receptor 1 on macrophages, activated the downstream JAK1 and STAT1/3 signalling and eventually led to PD‐L1 up‐expression. ATM inhibition augmented IR‐induced PD‐L1 expression in macrophages and CD8+ T cell infiltration, and promoted anti‐tumour efficacy in vivo. These results suggested that ATM inhibition promoted IR‐induced PD‐L1 expression through the activation of innate immunity in TAMs, which provided a novel approach to enhance the anti‐tumour efficacy of RT.
Early outcomes of complete excision followed by immediate postoperative single fractional 10 Gy for anterior chest keloids: A preliminary results
Authors: Ha Boram , Kim Sung Jae , Lee Yu Jun , Im Seongcheol , Park Tae Hwan 
Published: November 2022

AbstractTreatment of chest keloids is challenging. The aim of this study is to present our experience using surgical excision followed by postoperative single fractional 10 Gy radiotherapy for recurrent anterior chest keloids on the same day. 16 patients with recurrent anterior chest keloids were treated with complete excision followed by single‐fractional 10 Gy radiotherapy within eight hours postoperatively. The mean follow‐up period was 12 months. (10–14 months) The outcome was reported with a recurrence‐free rate. We also reported side effects. The overall recurrence‐free rate was 81.25%. The side effects were minimal, with two reported hyperpigmentation which was subsided after six months, and two cases of acute wound problems. Second malignancy or serious complications were not reported. Our preliminary results show positive outcomes of complete excision followed by postoperative single fractional 10 Gy radiotherapy for recurrent anterior chest keloids on the same day. Our current study needs further long‐term validation with more diverse patients.
Effect of La2O3 content on the oxygen barrier ability of the HfB2‐SiC coating at 1973 K
Authors: Qian Deyu , Chen Yuexing , Ren Xuanru , Wang Leyu , Chen Jiaping , Zhao Yan , Shi Dalin , Li Yuan , Du Haobo , Feng Peizhong 
Published: November 2022

AbstractTo inhibit the destructive evolution of the HfB2‐SiC coating during oxidation, La2O3 was added to modify the oxygen‐blocking capability of the HfB2‐SiC coating. The effect of La2O3 content on the oxygen barrier capacity of HfB2‐SiC was investigated. The addition of 5 vol.% La2O3 lowered the oxidation activity and strengthened the inert oxygen barrier capacity of the HfB2‐SiC coating, which made the oxygen permeability and maximum weight change rate of the coating decrease by 30.32% and 73.97%, respectively. Due to the solid solution reaction of the dispersed La2O3, HfO2, and SiO2 nanoparticles, a stable Hf‐B‐La‐Si‐O multiphase glass was formed on the surface of the coating. Besides, the high‐melting‐point particles, such as HfO2, La2Hf2O7, and HfSiO4, dispersed in the glass layer as hard particles and formed a mosaic structure based on the Hf‐B‐La‐Si‐O compound, which increased the cumulative protection efficiency of the HfB2‐SiC coating to 98.5% and reduced the inert factor value by 84.57%. However, the excessive consumption of SiO2 glass by La2O3 results in dendritization of the generated glass when the La2O3 content is above 10 vol.%, which reduced the self‐healing capability of the generated glass and instead weakened the oxygen barrier properties of the HfB2‐SiC‐La2O3 coatings.
Survey tools for measuring research or evidence‐based practice constructs in dietetics: A narrative review
Authors: Hand Rosa K. 
Published: November 2022

AbstractBackgroundExact quantification of research conducted by dietitians and tracking the effectiveness of interventions or training programs to increase either evidence‐based practice (EBP) or research behaviours have been hampered by the variety of tools used to measure these constructs.MethodsIn this narrative review, we identified and classified the various tools previously used to measure constructs related to research and/or EBP in the dietetics profession, and to summarise estimates of the constructs measured.ResultsWe identified and classified 15 scored tools that had been used at least once in the dietetics profession and extracted tool parameter estimates from 22 resulting papers. We also identified six unscored tools and three qualitative studies. The most frequent constructs measured were attitudes and behaviours. Very few tools measured objective knowledge (skills). No objective measures of research outputs were identified. Several tools were closely related to one another.ConclusionsEven when tools were used across multiple papers, reporting method varied making comparisons difficult. This review should encourage future researchers to utilise existing tools when possible, and encourage the development or adaptation and testing of tools that fill identified gaps. The constructs measured by the tools identified may also provide a starting point for the development of educational interventions aiming to increase research or EBP skills among dietitians. Only by using consistent tools will the dietetics profession be able to track the progress in increasing research conduct and EBP implementation over time.
Mutant SOD1 aggregates formed in vitro and in cultured cells are polymorphic and differ from those arising in the CNS
Authors: Nordström Ulrika , Lang Lisa , Ekhtiari Bidhendi Elaheh , Zetterström Per , Oliveberg Mikael , Danielsson Jens , Andersen Peter M. , Marklund Stefan L. 
Published: November 2022

AbstractMutations in the human Superoxide dismutase 1 (hSOD1) gene are well‐established cause of the motor neuron disease ALS. Patients and transgenic (Tg) ALS model mice carrying mutant variants develop hSOD1 aggregates in the CNS. We have identified two hSOD1 aggregate strains, which both transmit spreading template‐directed aggregation and premature fatal paralysis when inoculated into adult transgenic mice. This prion‐like spread of aggregation could be a primary disease mechanism in SOD1‐induced ALS. Human SOD1 aggregation has been studied extensively both in cultured cells and under various conditions in vitro. To determine how the structure of aggregates formed in these model systems related to disease‐associated aggregates in the CNS, we used a binary epitope‐mapping assay to examine aggregates of hSOD1 variants G93A, G85R, A4V, D90A, and G127X formed in vitro, in four different cell lines and in the CNS of Tg mice. We found considerable variability between replicate sets of in vitro‐generated aggregates. In contrast, there was a high similarity between replicates of a given hSOD1 mutant in a given cell line, but pronounced variations between different hSOD1 mutants and different cell lines in both structures and amounts of aggregates formed. The aggregates formed in vitro or in cultured cells did not replicate the aggregate strains that arise in the CNS. Our findings suggest that the distinct aggregate morphologies in the CNS could result from a micro‐environment with stringent quality control combined with second‐order selection by spreading ability. Explorations of pathogenesis and development of therapeutics should be conducted in models that replicate aggregate structures forming in the CNS.image
Veterinary healthcare needs to talk more about error: For the wellbeing of our patients and medical teams
Authors: Low Rochelle , Wu Albert W. 
Published: November 2022

AbstractOver the past 2 decades, patient safety has become an established priority in human healthcare. There is a large body of research in human medicine on harm caused by healthcare, its impact, and interventions to prevent it. There are also numerous guidelines, policies, and regulations to improve safety. An important realization has been that the same errors that harm patients can also harm members of the healthcare team. Empathetic handling of safety incidents can have positive effects on both the wellbeing of providers and their care of patients. An essential element in patient safety is the creation of a “culture of safety” within the health care team. A strong culture of safety describes a work environment where risk is acknowledged, individuals can report errors without fear of punishment, and the organization has a commitment to collaboratively implementing system changes to prevent future errors. A key element of safety culture is ensuring that healthcare team members are supported and asked to help create solutions for safer care. The principles of safety science and practices to improve safety have not yet been widely adopted in veterinary medicine. We describe a case of a serious medication error and how it was handled to illustrate key components of a culture of safety and a system‐based approach to improvement. This case is timely as a recent review of patient safety events in 3 veterinary hospitals found medication‐related errors to be the most frequently reported events. Open conversations about safety events and errors that can harm not only our patients but also our healthcare teams will help veterinary professionals learn from their mistakes, support members of the team, and prevent future harm.
Climate change adaptation in Central America: A review of the national policy efforts
Authors: Segura Luis D. , van Zeijl‐Rozema Annemarie , Martens Pim 
Published: November 2022

AbstractThe latest comparative studies of climate policies reinforce the fundamental importance of employing adaptation planning alongside national development strategies. While these studies are necessary to document and share the knowledge of the practices and experiences undertaken at the national level, documents are still scarce on regions of interest such as Central America. This article scrutinizes the state of adaptation planning through an exhaustive investigation of adaptation policy documents, consisting of National Adaptation Strategies, National Climate Legislation, and National Adaptation Action Plans, which are common in all six Central American countries. The findings reveal a region with an intricate yet progressive trend toward adaptation policies, with each country showing different speeds and qualities of adoption. An increasing learning curve has allowed for the emergence of two generations of adaptation policies with several improved features, including horizontal and vertical coordination, monitoring systems, and the inclusion of heterogeneous instruments to implement these systems. Yet, institutional challenges outside the adaptation realm could hinder the progress attained, due to factors such as political and economic crisis and institutional fragility. Further research into the broader political and governance landscape is needed, focusing on if and how climate policies should address political instability and institutional fragility as vulnerability stressors.
Visibly invisible: The study of middle class African American English
Authors: Weldon Tracey L. 
Published: November 2022

AbstractMiddle class African American English (AAE) has remained largely invisible to the sociolinguistic lens despite the fact that over 50 years of research has made it one of the most examined varieties of American English. This gap in the sociolinguistic literature is largely reflective of a strategic effort on the part of linguists to dismantle the stigma associated with working class vernacular varieties and improve outcomes for working class speakers who face linguistic discrimination in schools. An unfortunate by‐product of this laudable effort, however, has been the erasure of middle‐class speakers from our conceptualisations of the AAE speech community and a virtual obsession with the vernacular end of the AAE continuum. By interrogating the concept of the linguistic lame and giving greater attention to patterns of code‐switching and the ways in which talking Black or sounding Black get defined at the more standard end of the continuum, sociolinguists have an opportunity to broaden our understanding of AAE and its community of speakers and, in so doing, possibly extend our reach to a more diverse and inclusive audience of budding linguists.
Gut microbiome composition better reflects host phylogeny than diet diversity in breeding wood‐warblers
Authors: Baiz Marcella D. , Benavides C. Andrea , Miller Eliot T. , Wood Andrew W. , Toews David P. L. 
Published: November 2022

AbstractUnderstanding the factors that shape microbiomes can provide insight into the importance of host–symbiont interactions and on co‐evolutionary dynamics. Unlike for mammals, previous studies have found little or no support for an influence of host evolutionary history on avian gut microbiome diversity and instead have suggested a greater influence of the environment or diet due to fast gut turnover. Because effects of different factors may be conflated by captivity and sampling design, examining natural variation using large sample sizes is important. Our goal was to overcome these limitations by sampling wild birds to compare environmental, dietary and evolutionary influences on gut microbiome structure. We performed faecal metabarcoding to characterize both the gut microbiome and diet of 15 wood‐warbler species across a 4‐year period and from two geographical localities. We find host taxonomy generally explained ~10% of the variation between individuals, which is ~6‐fold more variation of any other factor considered, including diet diversity. Further, gut microbiome similarity was more congruent with the host phylogeny than with host diet similarity and we found little association between diet diversity and microbiome diversity. Together, our results suggest evolutionary history is the strongest predictor of gut microbiome differentiation among wood‐warblers. Although the phylogenetic signal of the warbler gut microbiome is not very strong, our data suggest that a stronger influence of diet (as measured by diet diversity) does not account for this pattern. The mechanism underlying this phylogenetic signal is not clear, but we argue host traits may filter colonization and maintenance of microbes.
Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation
Authors: KleinJan Hetty , Frioux Clémence , Califano Gianmaria , Aite Méziane , Fremy Enora , Karimi Elham , Corre Erwan , Wichard Thomas , Siegel Anne , Boyen Catherine , Dittami Simon M. 
Published: November 2022

AbstractMicrobes can modify their hosts' stress tolerance, thus potentially enhancing their ecological range. An example of such interactions is Ectocarpus subulatus, one of the few freshwater‐tolerant brown algae. This tolerance is partially due to its (un)cultivated microbiome. We investigated this phenomenon by modifying the microbiome of laboratory‐grown E. subulatus using mild antibiotic treatments, which affected its ability to grow in low salinity. Low salinity acclimation of these algal‐bacterial associations was then compared. Salinity significantly impacted bacterial and viral gene expression, albeit in different ways across algal‐bacterial communities. In contrast, gene expression of the host and metabolite profiles were affected almost exclusively in the freshwater‐intolerant algal‐bacterial communities. We found no evidence of bacterial protein production that would directly improve algal stress tolerance. However, vitamin K synthesis is one possible bacterial service missing specifically in freshwater‐intolerant cultures in low salinity. In this condition, we also observed a relative increase in bacterial transcriptomic activity and the induction of microbial genes involved in the biosynthesis of the autoinducer AI‐1, a quorum‐sensing regulator. This could have resulted in dysbiosis by causing a shift in bacterial behaviour in the intolerant algal‐bacterial community. Together, these results provide two promising hypotheses to be examined by future targeted experiments. Although they apply only to the specific study system, they offer an example of how bacteria may impact their host's stress response.