bluuebunny/crossref_metadata_2025_split huggingface dataset:
IL-1R/C3aR signaling regulates synaptic pruning in the prefrontal cortex of depression
Authors: Zhang Man-Man , Guo Min-Xia , Zhang Qiu-Ping , Chen Xue-Qin , Li Na-Zhi , Liu Qing , Cheng Jie , Wang Shi-Le , Xu Guang-Hui , Li Cheng-Fu , Zhu Ji-Xiao , Yi Li-Tao 
Published: June 2022

Abstract
Background
Major depressive disorder is characterized by not only monoamine neurotransmitters deficiencies but also persistent neuroinflammation. The complement system is an attractive therapeutic target for various inflammation-related diseases due to its early activation in inflammatory processes.

Results
In the present study, the dynamic alteration of complement C3 and its receptor C3aR during the occurrence of depression and the mechanism of astrocyte-microglia IL-1R/C3/C3aR on synaptic pruning were investigated. The proteomic analysis firstly showed that chronic stress caused an elevation of C3. GO analysis indicated that complement system-mediated synaptic pruning signaling was involved in depression. The dynamic observation indicated that C3/C3aR was activated in the early onset and throughout the course of depression induced by lipopolysaccharide (LPS) and chronic stress. In contrast, C3aR blockade inhibited the hyperactivation of microglial APT2/DHHC7 palmitoylation cycle, which mediated the translocation of STAT3 and the expression of proinflammatory cytokines. Meanwhile, C3aR blockade also attenuated the synaptic pruning and enhanced the synaptogenesis in the prefrontal cortex of mice. Moreover, the blockade of IL-1R/NF-κB signaling pathway reduced the release of C3 from astrocyte.

Conclusions
The current study demonstrates that astrocyte-microglia IL-1R/C3/C3aR activation causes the abnormal synaptic pruning in depression, and suggests that the activation of complement C3/C3aR may be particularly helpful in predicting the onset stage of depression.
Post-traumatic stress symptoms experienced by healthcare workers in Lebanon four months following Beirut’s ammonium nitrate explosion: a survey-based study
Authors: Bou Sanayeh Elie , El Chamieh Carolla , Saade Marie Christelle , Maalouf Rami George , Bizri Maya 
Published: June 2022

Abstract
Background
On August 4, 2020, Lebanon faced one of the deadliest mass casualty explosions the world has witnessed during the twenty-first century. The human and emotional tolls were heavy on attending physicians, clinical fellows, residents, interns, medical students, and registered nurses, who were working in dramatic conditions, triaging, and treating thousands of blast-related casualties. We evaluated the risk of developing post-traumatic stress disorder symptoms (PTSS), among these healthcare workers (HCWs) from different Lebanese hospitals.

Methods
This is a multicentered, cross-sectional study that was conducted in December 2020, using an online questionnaire that evaluated the risk of developing PTSS based on the validated self-reported PTSD-Checklist for DSM-V (PCL-5). We also explored possible correlates with the participants’ socio-demographic characteristics, job profile, mental health, and blast-related events.

Results
Out of 519 participants, 44% were at high risk of developing PTSS following Beirut-blast. Nurses, attending physicians, fellows, and participants who are older in age, married, or working at specific hospitals, were at a higher risk. Those identified at higher risk of PTSS were surgeons, anesthesiologists, emergency medicine doctors, or radiologists; and they were more likely to be willing to migrate; having a prior history of psychiatric medication intake for PTSD treatment, a prior history of PTSD, or a personal history of seeking mental health service. At last, the latter two parameters as well as the number of examined injuries, severe home damage, and testing positive for the COVID-19 virus during the two weeks’ period that followed the blast were found to be predictors for the development of PTSS.

Conclusion
Lebanese in-hospital HCWs were found to be at a high risk of developing PTSS following the Beirut-Blast, thus we recommend public health authorities to provide adequate resources to avoid the emergence of mental illnesses among these rescuers.
Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation
Authors: Ma Hao , Wang Lei , Yu Haojie , Wang Weiwei , Wu Guofang , Qin Guangyong , Tan Zhongfang , Wang Yanping , Pang Huili 
Published: June 2022

Abstract
Background
As an adequate plant protein raw material, soybean meal has attracted extensive attention because of its high protein content, abundant amino acids essential for animals, and many functional nutrients. Feed fermentation is a new way to improve nutrient absorption of animals by inoculating microorganisms, which can also play a probiotic role. In order to screen the lactic acid bacteria with remarkable protease-producing performance and excellent growth and fermentation characteristics, 1000 strains isolated from different materials were evaluated in this study. After primary and re-screening, 22 strains both with protein clear zone diameters larger than 15.00 mm (including the diameter of a hole puncher 10.00 mm) and protease activities greater than 20.00 U/mL were screened out for physiological and biochemical tests, as well as antimicrobial performance tests.

Results
Strains P (Pig) 15 and ZZUPF (Zhengzhou University Pig Fecal) 95, which have excellent physiological and biochemical characteristics, as well as good inhibition abilities for Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Micrococcus luteus, were selected and used as fermenting agents for 3- and 30-day (d) fermentation of soybean meal. The analysis of microorganisms, fermentation quality, and chemical composition during fermentation revealed that all lactic acid bacteria addition groups had lower harmful bacteria and pH value, as well as higher lactic acid bacteria content and lactic acid level compared with CK and protease-treated groups. These effects were even better especially after 30 days of fermentation.

Conclusions
This study indicated that the selected strains Lactiplantibacillus plantarum subsp. plantarum P15 and Enterococcus faecalis ZZUPF95 could be considered as potential probiotics for fermenting soybean meal for further research.

Graphical abstract
Post-PRRT scans: which scans to make and what to look for
Authors: Aalbersberg Else A. , de Vries–Huizing Daphne M. V. , Tesselaar Margot E. T. , Stokkel Marcel P. M. , Versleijen Michelle W. J. 
Published: June 2022

Abstract
Aim
The aim of this study was to evaluate the clinical utility of SPECT/CT (imaging of uptake in tumor lesions and additional findings) and the additional value of planar imaging in order to simplify clinical imaging protocols and decrease patients burden.

Materials and methods
One hundred consecutive patients with metastatic neuroendocrine tumor (NET) treated with PRRT were included. Post-therapy imaging was performed 24 h after each PRRT cycle by both whole-body planar imaging and abdominal- and thoracic SPECT/CT. All images were evaluated for (1) the presence of new lesions, (2) discordant lesions between the two acquisitions (planar or SPECT), (3) location of lesions on SPECT (abdominal, thoracic, or both), and (4) additional findings on non-contrast enhanced CT imaging.

Results
In total 368 PRRT cycles including post-therapy imaging were performed in 100 patients. 45 patients had abdominal disease only, whilst in 55 patients the disease was observed on both abdominal and thoracic SPECT. 16 patients had known bone lesions that were visible only on planar imaging as these were out of range of the SPECT/CT. During PRRT, one patient developed multiple new bone metastases after the second cycle of PRRT, which were visible on both planar and SPECT/CT images. In 11 patients additional findings were found on CT images, the most common and relevant being bowel obstruction, pleural effusion, and ascites. Patients who developed ascites during PRRT appeared to do extremely poor; a post-hoc analysis showed that overall survival was 13.2 months in patients that showed ascites during PRRT at any moment and 37.9 months in patients without ascites (p < 0.001).

Conclusion
From a clinical point of view, thoracoabdominal SPECT/CT imaging is the preferred method for post-PRRT imaging; planar imaging had no added value over SPECT/CT in this cohort. In patients with abdominal disease only on baseline imaging, SPECT/CT of the abdomen only might be sufficient for imaging during the PRRT course. All accompanying CT images should be reviewed for additional findings, especially ascites, which is suggested to be a poor prognostic factor in patients receiving PRRT.
A case of strongly suspected Lynch syndrome with colorectal neuroendocrine carcinoma
Authors: Kobayashi Naoya , Yoshida Hiroshi , Kawaguchi Shinya , Shiraso Satoru , Nemoto Noriko , Fujikawa Nanako , Haji Yoichi , Kono Emiko , Kokubo Shoji , Tsukuda Kazuhiko , Asano Shigeyuki , Shinya Fumiaki 
Published: June 2022

Abstract
Background
Neuroendocrine cell carcinomas (NEC) of the colon and rectum are uncommon, representing ~ 0.1% of all colorectal carcinomas. They are associated with a much worse prognosis compared to adenocarcinoma of the colon and rectum, as death occurs in approximately half of all patients within 1 year. Lynch syndrome (LS) is the most common cause of inherited colorectal cancer, accounting for 2–4% of newly diagnosed colorectal cancer cases. This case is extremely rare which was strongly suspected LS as the background, and NEC as the histological type of colorectal cancer.

Case presentation
The patient was a 44-year-old man presenting with vomiting as the main complaint. He had undergone ileocecal resection for cecal cancer at age 29. The diagnosis was obstructive descending colorectal cancer, and colonoscopy revealed tumors in the rectum and sigmoid colon in addition. Due to multiple occurrences of colorectal cancer and its prevalence in the patient’s family, LS was suspected. The operation which was a subtotal proctocolectomy was performed. Pathological analysis revealed complete curative resection and the descending colon cancer of the obstructed portion was at the most advanced pathological Stage IIIC in UICC TNM classification, and the tissue type was a NEC. The Ki-67 index was 70%. The results of the microsatellite instability (MSI) test showed high-frequency MSI. The BRAF V600E variant was negative. The immunoexpression of MLH1 was positive, MSH2 was negative, PMS2 was positive, and MSH6 was negative.

Conclusions
Extended surgery is recommended for incipient colorectal cancer in LS cases in order to reliably reduce the risk of developing metachronous colorectal cancer. The survival outcome of surgery alone on digestive tract NECs, even locoregional lesions that are completely resection, is extremely poor. It is currently unclear if digestive tract NECs develop more readily in patients with LS. The accumulation of additional cases is necessary.
Development and evaluation of a remote training strategy for the implementation of mental health evidence-based practices in rural schools: pilot study protocol
Authors: Eiraldi Ricardo , McCurdy Barry L. , Khanna Muniya S. , Goldstein Jessica , Comly Rachel , Francisco Jennifer , Rutherford Laura E. , Wilson Tara , Henson Kathryn , Farmer Thomas , Jawad Abbas F. 
Published: June 2022

Abstract
Background
An increasing number of schools in rural settings are implementing multi-tier positive behavioral interventions and supports (PBIS) to address school-climate problems. PBIS can be used to provide the framework for the implementation of evidence-based practices (EBPs) to address children’s mental health concerns. Given the large service disparities for children in rural areas, offering EBPs through PBIS can improve access and lead to better long-term outcomes. A key challenge is that school personnel need technical assistance in order to implement EBPs with fidelity and clinical effectiveness. Providing ongoing on-site support is not feasible or sustainable in the majority of rural schools, due to their remote physical location. For this reason, remote training technology has been recommended for providing technical assistance to behavioral health staff (BHS) in under-served rural communities.

Objectives
The purpose of this study is to use the user-centered design, guided by an iterative process (rapid prototyping), to develop and evaluate the appropriateness, feasibility, acceptability, usability, and preliminary student outcomes of two online training strategies for the implementation of EBPs at PBIS Tier 2.

Methods
The study will employ a pragmatic design comprised of a mixed-methods approach for the development of the training platform, and a hybrid type 2, pilot randomized controlled trial to examine the implementation and student outcomes of two training strategies: Remote Video vs. Remote Video plus Coaching.

Discussion
There is a clear need for well-designed remote training studies focused on training in non-traditional settings. Given the lack of well-trained mental health professionals in rural settings and the stark disparities in access to services, the development and pilot-testing of a remote training strategy for BHS in under-served rural schools could have a significant public health impact.

Ethics and dissemination
The project was reviewed and approved by the institutional review board. Results will be submitted to ClinicalTrials.gov and disseminated to community partners and participants, peer-reviewed journals, and academic conferences.

Trial registration
ClinicialTrials.gov, NCT05034198 and NCT05039164
Effect of retinol and α-tocopherol supplementation on photoreceptor and retinal ganglion cell apoptosis in diabetic rats model
Authors: Ichsan Andi Muhammad , Bukhari Agussalim , Lallo Subehan , Miskad Upik Anderiani , Dzuhry Andi Afdal , Islam Itzar Chaidir , Muhiddin Habibah Setyawati 
Published: June 2022

Abstract
Background
Diabetic retinopathy (DR) is the most common microvascular complication of diabetes. Retinol and α-tocopherol of diabetic models prevent the damage of photoreceptor and retinal ganglion cells (RGC) caused by hyperglycemia.

Objective
This study aims to examine the effect of retinol and α-tocopherol on photoreceptor and RGC densities and the expression of caspase-3 and -7 on the retinal layers of the diabetic rat model.

Methods
Alloxan 150 mg/kg body weight single dose was used to develop animal models, which were separated into eight groups. These consist of one group without intervention (group 1), one positive control with only induced alloxan (group 2), and others receiving retinol (group 3 and 6), α-tocopherol (group 4 and 7), or their combination (group 5 and 8). Furthermore, histopathological examination was performed using Hematoxylin–Eosin staining to evaluate the photoreceptor and RGC densities, while immunohistochemistry staining evaluated the caspase-3 and -7 expressions.

Results
In the treatment group, the highest and lowest densities were identified in diabetic rats given α-tocopherol (group 7) and retinol (group 3) respectively. The caspase-3 and -7 expression showed that the group given α-tocopherol (group 7) had the lowest value.

Conclusion
In diabetic rats, retinol and α-tocopherol compounds maintained densities and prevented photoreceptor and RGC death. However, α-tocopherol was more promising than retinol or combinations in the prevention of retinal cells apoptosis.
Habit-like attentional bias is unlike goal-driven attentional bias against spatial updating
Authors: Hong Injae , Kim Min-Shik 
Published: June 2022

AbstractStatistical knowledge of a target’s location may benefit visual search, and rapidly understanding the changes in regularity would increase the adaptability in visual search situations where fast and accurate performance is required. The current study tested the sources of statistical knowledge—explicitly-given instruction or experience-driven learning—and whether they affect the speed and location spatial attention is guided. Participants performed a visual search task with a statistical regularity to bias one quadrant (“old-rich” condition) in the training phase, followed by another quadrant (“new-rich” condition) in the switching phase. The “instruction” group was explicitly instructed on the regularity, whereas the “no-instruction” group was not. It was expected that the instruction group would rely on goal-driven attention (using regularities with explicit top-down knowledge), and the no-instruction group would rely on habit-like attention (learning regularities through repetitive experiences) in visual search. Compared with the no-instruction group, the instruction group readjusted spatial attention following the regularity switch more rapidly. The instruction group showed greater attentional bias toward the new-rich quadrant than the old-rich quadrant; however, the no-instruction group showed a similar extent of attentional bias to two rich quadrants. The current study suggests that the source of statistical knowledge can affect attentional allocation. Moreover, habit-like attention, a different type of attentional source than goal-driven attention, is relatively implicit and inflexible.
On the surplus accuracy of data-driven energy quantification methods in the residential sector
Authors: Wederhake Lars , Wenninger Simon , Wiethe Christian , Fridgen Gilbert 
Published: June 2022

AbstractIncreasing trust in energy performance certificates (EPCs) and drawing meaningful conclusions requires a robust and accurate determination of building energy performance (BEP). However, existing and by law prescribed engineering methods, relying on physical principles, are under debate for being error-prone in practice and ultimately inaccurate. Research has heralded data-driven methods, mostly machine learning algorithms, to be promising alternatives: various studies compare engineering and data-driven methods with a clear advantage for data-driven methods in terms of prediction accuracy for BEP. While previous studies only investigated the prediction accuracy for BEP, it yet remains unclear which reasons and cause–effect relationships lead to the surplus prediction accuracy of data-driven methods. In this study, we develop and discuss a theory on how data collection, the type of auditor, the energy quantification method, and its accuracy relate to one another. First, we introduce cause–effect relationships for quantifying BEP method-agnostically and investigate the influence of several design parameters, such as the expertise of the auditor issuing the EPC, to develop our theory. Second, we evaluate and discuss our theory with literature. We find that data-driven methods positively influence cause–effect relationships, compensating for deficits due to auditors’ lack of expertise, leading to high prediction accuracy. We provide recommendations for future research and practice to enable the informed use of data-driven methods.
Comparative performance of hybrid generations reveals the potential application of F2 hybrids in upland cotton
Authors: CHEN Liangliang , TANG Huini , ZHANG Xuexian , QI Tingxiang , GUO Liping , SHAHZAD Kashif , WANG Hailin , QIAO Xiuqin , ZANG Rong , ZHANG Meng , WU Jianyong , XING Chaozhu 
Published: June 2022

Abstract
Background
The utilization of heterosis has greatly improved the productivity of cotton worldwide. However, a major constraint for the large-scale promotion of F1 hybrid cotton is artificial emasculation and pollination. This study proposed the potential utilization of F2 hybrids to improve upland cotton production through a comparative evaluation of hybrid generations.

Results
Eight upland cotton varieties were analyzed and crosses were made according to NCII incomplete diallel cross-breeding design in two cotton belts of China. Variance analysis revealed significant differences in agronomic, yield, and fiber quality in both generations and environments. The broad-sense heritability of agronomic and yield traits was relatively higher than quality traits. Furthermore, the narrow-sense heritability of some traits was higher in F2 than in the F1 generation in both cotton belts. Overall, parental lines Zhong901, ZB, L28, and Z98 were observed with maximum combining ability while combinations with strong special combining ability were ZB × DT, L28 × Z98, and ZB × 851. The yield traits heterosis was predominant in both generations. However, the level of heterosis was altered with trait, hybrid combination, generation, and environment. Interestingly, L28 × Z98 performed outstandingly in Anyang. Its lint yield (LY) was 24.2% higher in F1 and 11.6% in F2 than that of the control Ruiza 816. The performance of SJ48 × Z98 was excellent in Aral which showed 36.5% higher LY in F1 and 10.9% in F2 than control CCRI 49. Further results revealed most hybrid combinations had shown a low level of heterosis for agronomic and fiber quality traits in both generations. Comparatively, ZB × DT and L28 × Z98 showed hybrid vigor for multiple traits in both generations and cotton belts. It is feasible to screen strong heterosis hybrid combinations with fine fiber in early generations. In the two environments, the correlation of some traits showed the same trend, and the correlation degree of Anyang site was higher than that of Aral site, and the correlation of some traits showed the opposite trend. According to the performance of strong heterosis hybrid combinations in different environments, the plant type, yield and fiber traits associated with them can be improved according to the correlation.

Conclusions
Through comparative analysis of variance, combining ability, and heterosis in F1 and F2 hybrids in different cotton belts, this study proposed the potential utilization of F2 hybrids to improve upland cotton productivity in China.
A positive feedback regulatory loop, SA-AtNAP-SAG202/SARD1-ICS1-SA, in SA biosynthesis involved in leaf senescence but not defense response
Authors: Wang Yaxin , Liu Bin , Hu Youzhen , Gan Su-Sheng 
Published: June 2022

AbstractSalicylic acid (SA) is an important plant hormone that regulates defense responses and leaf senescence. It is imperative to understand upstream factors that regulate genes of SA biosynthesis. SAG202/SARD1 is a key regulator for isochorismate synthase 1 (ICS1) induction and SA biosynthesis in defense responses. The regulatory mechanism of SA biosynthesis during leaf senescence is not well understood. Here we show that AtNAP, a senescence-specific NAC family transcription factor, directly regulates a senescence-associated gene named SAG202 as revealed in yeast one-hybrid and in planta assays. Inducible overexpreesion of AtNAP and SAG202 lead to high levels of SA and precocious senescence in leaves. Individual knockout mutants of sag202 and ics1 have markedly reduced SA levels and display a significantly delayed leaf senescence phenotype. Furthermore, SA positively feedback regulates AtNAP and SAG202. Our research has uncovered a unique positive feedback regulatory loop, SA-AtNAP-SAG202-ICS1-SA, that operates to control SA biosynthesis associated with leaf senescence but not defense response.