bluuebunny/crossref_metadata_2025_split huggingface dataset:
The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato
Authors: Xue Luyao , Wei Zihao , Zhai Hong , Xing Shihan , Wang Yuxin , He Shaozhen , Gao Shaopei , Zhao Ning , Zhang Huan , Liu Qingchang 
Published: September 2022

Summary

Drought limits crop development and yields. bHLH (basic helix–loop–helix) transcription factors play critical roles in regulating the drought response in many plants, but their roles in this process in sweet potato are unknown.

Here, we report that two bHLH proteins, IbbHLH118 and IbbHLH66, play opposite roles in the ABA‐mediated drought response in sweet potato. ABA treatment repressed IbbHLH118 expression but induced IbbHLH66 expression in the drought‐tolerant sweet potato line Xushu55‐2. Overexpressing IbbHLH118 reduced drought tolerance, whereas overexpressing IbbHLH66 enhanced drought tolerance, in sweet potato.

IbbHLH118 directly binds to the E‐boxes in the promoters of ABA‐insensitive 5 (IbABI5), ABA‐responsive element binding factor 2 (IbABF2) and tonoplast intrinsic protein 1 (IbTIP1) to suppress their transcription. IbbHLH118 forms homodimers with itself or heterodimers with IbbHLH66. Both of the IbbHLHs interact with the ABA receptor IbPYL8. ABA accumulates under drought stress, promoting the formation of the IbPYL8–IbbHLH66–IbbHLH118 complex. This complex interferes with IbbHLH118's repression of ABA‐responsive genes, thereby activating ABA responses and enhancing drought tolerance.

These findings shed light on the role of the IbPYL8–IbbHLH66–IbbHLH118 complex in the ABA‐dependent drought response of sweet potato and identify candidate genes for developing elite crop varieties with enhanced drought tolerance.
The role of volunteer experience on performance on online volunteering platforms
Authors: Urrea Gloria , Yoo Eunae 
Published: September 2022

Online volunteering platforms allow humanitarian organizations (HOs) to recruit volunteers to work remotely on projects of varying urgency. While removing time and space constraints enables HOs to scale up their volunteer force, HOs must manage greater heterogeneity in volunteers' experience. We investigate empirically how volunteers' experience impacts two performance metrics on online volunteering platforms: project completion rate and volunteer retention. In addition, we examine the conditions under which experience becomes more relevant to project completion rate and retention. We collected a novel panel data set from the Humanitarian OpenStreetMap Team Tasking Manager. Our data set includes 5,162 online volunteering projects with 2,169,683 contributions by 96,450 volunteers. Results from a panel regression model show that a project's completion rate improves in diminishing increments with the experience of the contributing volunteers. We further find that the effect of experience on project completion rate is contingent on the urgency of the project. Regarding retention, results from a parametric hazard model indicate that volunteers are incentivized to return to an online volunteering platform more quickly when they are closer to attaining a new experience‐based rank. However, we find that this effect weakens as volunteers gain experience. Overall, our study sheds light on volunteer management in an online context and offers operational insights on the recruitment and allocation of volunteers for HOs as well as online volunteering platforms.
The version effect of apps and operating systems in mobile commerce
Authors: Zhang Xingyue (Luna) , Cui Ruomeng , Yao Oliver 
Published: September 2022

Different versions of mobile operating systems or shopping apps enable different functionalities and information flows, thus creating various mobile shopping environments. In general, up‐to‐date versions provide better information flows and richer functionalities. However, mobile operating systems and shopping apps affect consumer behavior through different mechanisms. An up‐to‐date mobile operating system reduces the system response time, while an up‐to‐date shopping app improves algorithms for better search accuracy. The former encourages consumer explore and search, whereas the latter improves consumer search efficiency and reduces chances for consumers to discover more products. Using a unique large‐scale clickstream data set from a mobile commerce retailer, we examine the effect of mobile operating system and app versions on consumer search and impulse purchase behaviors in mobile commerce. Our results show that consumers with an up‐to‐date mobile operating system or a previous version of a shopping app conduct more searches in terms of increased product page views and time spent on product pages, which results in a higher probability of consumer impulse purchase. However, consumers' search for individual products is not affected by versions. Surprisingly, though more page views or time spent may boost purchases, we find that consumer search affects impulse purchases nonlinearly in that page views and time spent have a decreasing rate of impact. Our computations show that using an up‐to‐date operating system increases consumer search activities by 60.45 product pages or 521.30 seconds spent browsing compared to using a previous version of an operation system. Using an up‐to‐date app decreases consumer search activities by 129.34 product pages or 1446.48 seconds spent browsing compared to using a previous version of a mobile app.
Experimental Investigation of the Oil Supply Layer in a Model Rolling Bearing
Authors: Liang He , Fan Zhihan , Wang Wenzhong , Zhao Ziqiang 
Published: September 2022

Abstract
Most of the rolling bearings work under starvation conditions. The quantitative measurement of the oil layer may provide a new perspective on the rolling bearing lubrication. In this research, the laser-induced fluorescence technique was utilized to measure the oil layer thickness out of the contact on a modified angular contact ball-bearing testing apparatus. The forms of oil supply for the ball-outer-ring contact at various speeds were studied, as well as the influences of centrifugal forces.
A Proof-of-Concept Study on an Internal-Resonance-Based Piezoelectric Energy Harvester With Coupled Three-Dimensional Bending-Torsion Motions
Authors: Fan Yimin , Ghayesh Mergen H. , Lu Tien-Fu 
Published: September 2022

Abstract
By exchanging the internal energy between coupled vibration modes, internal-resonance-based energy harvesters may provide an effective solution to broadening and enhancing bandwidth and power performance in dealing with natural vibration sources. With the development of piezoelectric-based transducers, thickness and face shear coefficients in proper piezoelectric elements can also generate power output from shear deformation on the core vibrating elements. However, in most cantilever-based energy harvesters that focused on bending modes, the shear responses were neglected. In this paper, we present an internal-resonance-based piezoelectric energy harvester with three-dimensional coupled bending and torsional modes, for the first time. The fine-tuned system leverages a two-to-one internal resonance between its first torsion and second bending modes to enhance the power output with piezoelectric effects. The dynamic behavior implies the coexistence of in-plane and out-of-plane motions under a single excitation frequency, and the corresponding strain changes in the bending and shear directions are captured by bonded piezoelectric transducers. Dependence between excitation levels and the internal-resonance phenomenon is justified as a critical system parameter study; the results also indicate that an intriguing non-periodic region exists near the center frequency. The outcomes of this study feature a multi-directional and multi-modal energy harvester that displays rich dynamic behaviors. The operational bandwidth is promising for broadband energy harvesting, and the output voltage is enhanced by capturing both in-plane and out-of-plane motions at the same time.
Broadband Whistler Waves and Differential Electron Fluxes in the Equatorial Region of the Magnetosphere behind the Plasmapause during Substorm Injections
Authors: Titova E. E. , Shklyar D. R. , Manninen J. 
Published: September 2022

Abstract
This paper considers the whistler waves in the frequency band from 3 to 30 kHz observed on the Van Allen Probe-B satellite on March 17, 2019, when the satellite was on L-shells from 2.8 to 5.4. The upper frequency in the emission spectrum followed the course of the electron gyrofrequency $${{f}_{{ce}}}$$ and was lower than it by 1–5 kHz. The emission spectrum often had two spectral maxima (above and below fce/2); the maximum at frequencies above fce/2 could be either more or less intense. High-frequency whistler waves at frequencies > fce/2 were observed simultaneously with an increase in low-energy electron fluxes with energies more than 102 eV, which had transverse anisotropy. To explain the observed spectrum, we used simultaneous satellite measurements of the cold plasma density and differential fluxes of energetic electrons in the energy range from 0.015 to 250 keV in a wide range of pitch angles to determine the electron distribution function and calculate local linear growth rate as a function of frequency f and wave normal angle $$\theta $$. The calculations were performed for three cyclotron resonances (n = 1, 0, –1) that make the largest contributions to the wave growth rate. The calculations showed the presence of a pronounced maximum at frequencies (0.8–0.9) fce. The energy range and pitch angles of electrons with a maximum contribution to wave excitation at these frequencies were estimated.
Learning health systems in low-income and middle-income countries: exploring evidence and expert insights
Authors: Witter Sophie , Sheikh Kabir , Schleiff Meike 
Published: September 2022

IntroductionLearning health systems (LHS) is a multifaceted subject. This paper reviewed current concepts as well as real-world experiences of LHS, drawing on published and unpublished knowledge in order to identify and describe important principles and practices that characterise LHS in low/middle-income country (LMIC) settings.MethodsWe adopted an exploratory approach to the literature review, recognising there are limited studies that focus specifically on system-wide learning in LMICs, but a vast set of connected bodies of literature. 116 studies were included, drawn from an electronic literature search of published and grey literature. In addition, 17 interviews were conducted with health policy and research experts to gain experiential knowledge.ResultsThe findings were structured by eight domains on learning enablers. All of these interact with one another and influence actors from community to international levels.We found that learning comes from the connection between information, deliberation, and action. Moreover, these processes occur at different levels. It is therefore important to consider experiential knowledge from multiple levels and experiences. Creating spaces and providing resources for communities, staff and managers to deliberate on their challenges and find solutions has political implications, however, and is challenging, particularly when resources are constrained, funding and accountability are fragmented and the focus is short-term and narrow. Nevertheless, we can learn from countries that have managed to develop institutional mechanisms and human capacities which help health systems respond to changing environments with ‘best fit’ solutions.ConclusionHealth systems are knowledge producers, but learning is not automatic. It needs to be valued and facilitated. Everyday governance of health systems can create spaces for reflective practice and learning within routine processes at different levels. This article highlights important enablers, but there remains much work to be done on developing this field of knowledge.
Driving an innovation contest into crisis
Authors: Romanov Aleksandr 
Published: September 2022

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Aleksandr Romanov considers how a lack of permanent leadership is impacting the InnovateFPGA contest.
Neoadjuvant Chemotherapy Is Associated with Altered Immune Cell Infiltration and an Anti-Tumorigenic Microenvironment in Resected Pancreatic Cancer
Authors: Dias Costa Andressa , Väyrynen Sara A. , Chawla Akhil , Zhang Jinming , Väyrynen Juha P. , Lau Mai Chan , Williams Hannah L. , Yuan Chen , Morales-Oyarvide Vicente , Elganainy Dalia , Singh Harshabad , Cleary James M. , Perez Kimberly , Ng Kimmie , Freed-Pastor William , Mancias Joseph D. , Dougan Stephanie K. , Wang Jiping , Rubinson Douglas A. , Dunne Richard F. , Kozak Margaret M. , Brais Lauren , Reilly Emma , Clancy Thomas , Linehan David C. , Chang Daniel T. , Hezel Aram F. , Koong Albert C. , Aguirre Andrew J. , Wolpin Brian M. , Nowak Jonathan A. 
Published: September 2022

Abstract

Purpose:
Neoadjuvant chemotherapy is increasingly administered to patients with resectable or borderline resectable pancreatic ductal adenocarcinoma (PDAC), yet its impact on the tumor immune microenvironment is incompletely understood.

Experimental

Design:
We employed quantitative, spatially resolved multiplex immunofluorescence and digital image analysis to identify T-cell subpopulations, macrophage polarization states, and myeloid cell subpopulations in a multi-institution cohort of up-front resected primary tumors (n = 299) and in a comparative set of resected tumors after FOLFIRINOX-based neoadjuvant therapy (n = 36) or up-front surgery (n = 30). Multivariable-adjusted Cox proportional hazards models were used to evaluate associations between the immune microenvironment and patient outcomes.

Results:
In the multi-institutional resection cohort, immune cells exhibited substantial heterogeneity across patient tumors and were located predominantly in stromal regions. Unsupervised clustering using immune cell densities identified four main patterns of immune cell infiltration. One pattern, seen in 20% of tumors and characterized by abundant T cells (T cell–rich) and a paucity of immunosuppressive granulocytes and macrophages, was associated with improved patient survival. Neoadjuvant chemotherapy was associated with a higher CD8:CD4 ratio, greater M1:M2–polarized macrophage ratio, and reduced CD15+ARG1+ immunosuppressive granulocyte density. Within neoadjuvant-treated tumors, 72% showed a T cell–rich pattern with low immunosuppressive granulocytes and macrophages. M1-polarized macrophages were located closer to tumor cells after neoadjuvant chemotherapy, and colocalization of M1-polarized macrophages and tumor cells was associated with greater tumor pathologic response and improved patient survival.

Conclusions:
Neoadjuvant chemotherapy with FOLFIRINOX shifts the PDAC immune microenvironment toward an anti-tumorigenic state associated with improved patient survival.
Preclinical Evaluation of 225Ac-Labeled Single-Domain Antibody for the Treatment of HER2pos Cancer
Authors: Rodak Magdalena , Dekempeneer Yana , Wojewódzka Maria , Caveliers Vicky , Covens Peter , Miller Brian W. , Sevenois Matthijs B. , Bruchertseifer Frank , Morgenstern Alfred , Lahoutte Tony , D'Huyvetter Matthias , Pruszyński Marek 
Published: September 2022

Abstract
Human epidermal growth factor receptor type 2 (HER2) is overexpressed in various cancers; thus, HER2-targeting single-domain antibodies (sdAb) could offer a useful platform for radioimmunotherapy. In this study, we optimized the labeling of an anti-HER2-sdAb with the α-particle-emitter 225Ac through a DOTA-derivative. The formed radioconjugate was tested for binding affinity, specificity and internalization properties, whereas cytotoxicity was evaluated by clonogenic and DNA double-strand-breaks assays. Biodistribution studies were performed in mice bearing subcutaneous HER2pos tumors to estimate absorbed doses delivered to organs and tissues. Therapeutic efficacy and potential toxicity were assessed in HER2pos intraperitoneal ovarian cancer model and in healthy C57Bl/6 mice. [225Ac]Ac-DOTA-2Rs15d exhibited specific cell uptake and cell-killing capacity in HER2pos cells (EC50 = 3.9 ± 1.1 kBq/mL). Uptake in HER2pos lesions peaked at 3 hours (9.64 ± 1.69% IA/g), with very low accumulation in other organs (&lt;1% IA/g) except for kidneys (11.69 ± 1.10% IA/g). α-camera imaging presented homogeneous uptake of radioactivity in tumors, although heterogeneous in kidneys, with a higher signal density in cortex versus medulla. In mice with HER2pos disseminated tumors, repeated administration of [225Ac]Ac-DOTA-2Rs15d significantly prolonged survival (143 days) compared to control groups (56 and 61 days) and to the group treated with HER2-targeting mAb trastuzumab (100 days). Histopathologic evaluation revealed signs of kidney toxicity after repeated administration of [225Ac]Ac-DOTA-2Rs15d. [225Ac]Ac-DOTA-2Rs15d efficiently targeted HER2pos cells and was effective in treatment of intraperitoneal disseminated tumors, both alone and as an add-on combination with trastuzumab, albeit with substantial signs of inflammation in kidneys. This study warrants further development of [225Ac]Ac-DOTA-2Rs15d.
Tumor-Infiltrating Myeloid Cells Confer De Novo Resistance to PD-L1 Blockade through EMT–Stromal and Tgfβ-Dependent Mechanisms
Authors: Yu Haocheng , Sfakianos John P. , Wang Li , Hu Yang , Daza Jorge , Galsky Matthew D. , Sandhu Harkirat S. , Elemento Olivier , Faltas Bishoy M. , Farkas Adam M. , Bhardwaj Nina , Zhu Jun , Mulholland David J. 
Published: September 2022

Abstract
Most bladder cancers are poorly responsive to immune checkpoint blockade (ICB). With the need to define mechanisms of de novo resistance, including contributions from the tumor microenvironment (TME), we used single-cell transcriptional profiling to map tumor-infiltrating lymphocytic and myeloid cells in 10 human bladder tumors obtained from patients with a history of smoking either with or without previous ICB. Human datasets were qualitatively compared with single cell datasets from the BBN carcinogen-induced mouse model of bladder cancer, which was poorly responsive to PD-L1 blockade. We applied an established signature of acquired ICB resistance to these human and murine datasets to reveal conservation in EMT and TGFβ ICB resistance signatures between human–mouse stromal and myeloid cells. Using TCGA transcriptional datasets and deconvolution analysis, we showed that patients with a history of smoking and bladder tumors high in M2 macrophage tumor content had a significantly worse survival outcome compared with nonsmokers who were M2 high. Similarly, BBN-induced tumors were high in M2 macrophage content and contained exhausted T–NK cells, thereby modeling the identified TCGA patient subpopulation. The combined targeting of TGFβ + PD-L1 reverted immune cell exclusion and resulted in increased survival and delayed BBN-induced tumor progression. Together, these data support a coordinated role for stromal and myeloid cell populations in promoting de novo resistance to PD-L1 blockade, particularly in patients with a history of smoking.

Significance:
Most patients with bladder cancer do not respond to ICB targeting of the PD-L1 signaling axis. Our modeling applied a de novo resistance signature to show that tumor-infiltrating myeloid cells promote poor treatment response in a TGFβ-dependent mechanism.
Hue dual-chromatic calcium chlorosilicate phosphor for light-emitting diode having yellow and white illumination
Authors: Phuc Dang Huu , Van Hien Bui , Le Thai Nguyen 
Published: September 2022

<p><span>High-thermal solid phase reactions produced a series of bright green-yellow phosphors called Ca<sub>3</sub>SiO<sub>4</sub>Cl<sub>2</sub>,Eu<sup>2+</sup>,Mn<sup>2+ </sup>(CaM). For example, powder dispersed reflection, photoluminescence excitation (PE), and along with emission spectra, were used to determine their luminous characteristics at between 10 K and 450 K of temperature. While the CaM exhibits wide absorption bands in the 250-450 nm range, which matches the near-uv of the InGaN-based chips, they also display two dominant absorptions, attributed to the 5d → 4f Eu<sup>2+</sup> transformation, as well as the <sup>4</sup>T<sub>1g</sub>(<sup>4</sup>G) → <sup>6</sup>A<sub>1g</sub>(<sup>6</sup>S) transfer of the two ions Eu<sup>2+</sup> and Mn<sup>2+</sup>, respectively. Increasing the concentration of Mn<sup>2+</sup> ion reduces the lifespan of the Eu<sup>2+</sup> ion. This encourages an efficient energy exchange between these two ions. The UV light leakage issue in yellow LEDs was resolved by intergrating with n-UV InGaN chips and CaM. Powerful white LEDs were then created by mixing blue chlorophosphate with CaM. Its color coordinate was (0.3281,0.3071), and the associated color temperature (TC) was 6065 K, while the general hue rendering (Ra) and illuminating effectiveness (11 lm/W) were respectively 84.5 and 11 lm/W.</span></p>
БОЛЬОВИЙ СИНДРОМ ТА ФУНКЦІОНАЛЬНІ РОЗЛАДИ ПЛЕЧОВОГО СУГЛОБА В ПРАКТИЦІ СІМЕЙНОГО ЛІКАРА
Authors: Грубар Ю. О. , Грубар М. Ю. 
Published: September 2022

РЕЗЮМЕ. Біль у плечовому суглобі є третьою із найпоширеніших причин звертань пацієнтів з патологією опорно-рухового апарату до первинної ланки медичної допомоги. Різноманітність форм патології викликає труднощі з клінічною діагностикою, показаннями до інструментальних методів обстеження, оцінкою отриманих результатів та вибором лікувальної тактики, особливо на первинній ланці медичної допомоги.
Мета – оцінити результати клінічного обстеження і діагностичних заходів, спрямованих на визначення патології періартикулярних тканин ділянки плечового суглоба, та розробити алгоритм послідовності дій лікаря загальної практики – сімейної медицини в діагностиці патології періартикулярних тканин плечового суглоба.
Матеріал і методи. В ортопедично-травматологічному відділення КНП «Тернопільська обласна клінічна лікарня» ТОР за період з 2013 до 2021 р. проведено обстеження та лікування 362 пацієнтів із патологією періартикулярних тканин плечового суглоба.
Результати. Патологію ротаторної манжети плеча виявлено в 138 (38,12 %) пацієнтів, адгезивний капсуліт у 92 (25,42 %) хворих; у 74 (20,44 %) пацієнтів діагностовано кальцифікуючий тендиніт плеча, тендопатія сухожилка двоголового м’яза плеча була в 23 (6,36 %) пацієнтів. Інша патологія плечового суглоба виявлена у 35 (9,67 %) пацієнтів.
Висновки. Обстеження пацієнта з патологією періартикулярних тканин плечового суглоба повинно будуватися за наступним алгортмом: ретельний збір анамнезу; детальне вивчення скарг пацієнта; аналіз причин больового синдрому; аналіз причин рухових розладів. Огляд таких пацієнтів необхідно проводити ретельно, він повинен бути порівняльним, з застосуванням специфічних провокаційних тестів.