bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microorganisms harbor keys to a circular bioeconomy making them useful tools in fighting plastic pollution and rising CO2 levels
Authors: Antranikian Garabed , Streit Wolfgang R. 
Published: February 2022

AbstractThe major global and man-made challenges of our time are the fossil fuel-driven climate change a global plastic pollution and rapidly emerging plant, human and animal infections. To meet the necessary global changes, a dramatic transformation must take place in science and society. This transformation will involve very intense and forward oriented industrial and basic research strongly focusing on (bio)technology and industrial bioprocesses developments towards engineering a zero-carbon sustainable bioeconomy. Within this transition microorganisms—and especially extremophiles—will play a significant and global role as technology drivers. They harbor the keys and blueprints to a sustainable biotechnology in their genomes. Within this article, we outline urgent and important areas of microbial research and technology advancements and that will ultimately make major contributions during the transition from a linear towards a circular bioeconomy.
Renal Protection with SGLT2 Inhibitors: Effects in Acute and Chronic Kidney Disease
Authors: Bailey Clifford J. , Day Caroline , Bellary Srikanth 
Published: February 2022

AbstractPurpose of ReviewThis review offers a critical narrative evaluation of emerging evidence that sodium-glucose co-transporter-2 (SGLT2) inhibitors exert nephroprotective effects in people with type 2 diabetes.Recent FindingsThe SGLT2 inhibitor class of glucose-lowering agents has recently shown beneficial effects to reduce the onset and progression of renal complications in people with and without diabetes. Randomised clinical trials and ‘real world’ observational studies, mostly involving type 2 diabetes patients, have noted that use of an SGLT2 inhibitor can slow the decline in glomerular filtration rate (GFR), reduce the onset of microalbuminuria and slow or reverse the progression of proteinuria.SummaryThe nephroprotective effects of SGLT2 inhibitors are class effects observed with each of the approved agents in people with a normal or impaired GFR. These effects are also observed in non-diabetic, lean and normotensive individuals suggesting that the mechanisms extend beyond the glucose-lowering, weight-lowering and blood pressure-lowering effects that accompany their glucosuric action in diabetes patients. A key mechanism is tubuloglomerular feedback in which SGLT2 inhibitors cause more sodium to pass along the nephron: the sodium is sensed by macula cells which act via adenosine to constrict afferent glomerular arterioles, thereby protecting glomeruli by reducing intraglomerular pressure. Other effects of SGLT2 inhibitors improve tubular oxygenation and metabolism and reduce renal inflammation and fibrosis. SGLT2 inhibitors have not increased the risk of urinary tract infections or the risk of acute kidney injury. However, introduction of an SGLT2 inhibitor in patients with a very low GFR is not encouraged due to an initial dip in GFR, and it is prudent to discontinue therapy if there is an acute renal event, hypovolaemia or hypotension.
Phenotypical screening on metastatic PRCC-TFE3 fusion translocation renal cell carcinoma organoids reveals potential therapeutic agents
Authors: Cao Chuanzhen , Lan Xiaomei , Shang Bingqing , Jiang Weixing , Guo Lei , Zheng Shan , Bi Xingang , Zhou Aiping , Sun Zhijian , Shou Jianzhong 
Published: February 2022

Abstract
Purpose
Translocation renal cell carcinoma (tRCC) is a subtype that occurs predominantly in children and young individuals. Metastatic tRCC occurring in young patients is more aggressive than that occurring in older patients, and there are still no effective therapies. Organoids can mimic original tissues and be assessed by high-throughput screening (HTS). We aimed to utilize patient-derived organoids and HTS to screen drugs that can be repurposed for metastatic tRCC with PRCC-TFE3 fusion.

Methods
Tumor tissues were obtained from treatment-naïve metastatic tRCC patients who underwent surgery. Histopathology and fluorescence in situ hybridization (FISH) confirmed the tRCC. Organoids derived from the dissected tissues were cultured and verified by FISH and RNA-seq. HTS was performed to seek promising drugs, and potential mechanisms were explored by RNA-seq and cell-based studies.

Results
We successfully established a metastatic tRCC organoid with PRCC-TFE3 fusion, a common fusion subtype, and its characteristics were verified by histopathology, FISH, and RNA-seq. An HTS assay was developed, and the robustness was confirmed. A compound library of 1816 drugs was screened. Eventually, axitinib, crizotinib, and JQ-1 were selected for further validation and were found to induce cell cycle arrest and apoptosis. RNA-seq analyses of posttreatment organoids indicated that crizotinib induced significant changes in autophagy-related genes, consistent with the potential pathogenesis of tRCC.

Conclusions
We established and validated organoids derived from tissues dissected from a patient with metastatic tRCC with PRCC-TFE3 fusion and achieved the HTS process for the first time. Crizotinib might be a targeted therapy worthy of exploration in the clinic for metastatic tRCC with PRCC-TFE3 fusion. Such organoid and HTS assays may represent a promising model system in translational research assisting in the development of clinical strategies.
Experienced Quality of Life and Cultural Activities in Elderly Care
Authors: Koponen Tuulia , Löyttyniemi Eliisa , Arve Seija , Honkasalo Marja-Liisa , Rautava Päivi 
Published: February 2022

AbstractWe investigated if cultural activities are associated with improved quality of life experiences of older people. In 2012, older people in five care units were invited to participate more cultural activities (study group) than usual. Each person in the study group had a tailored cultural plan integrated into the care plan. Older people in traditional care units (control group) did not have such cultural plans.One hundred sixty-one persons from care units in two cities in Finland participated in 2012 and 161 persons in 2014 in a cross-sectional study. Their quality of life was assessed with the World Health Organization’s Quality of Life WHOQOL-BREF (Field Tríal Version) enquiry. The quality of life variable contained four domains: physical, psychosocial, social and environment. The values of these domains underwent multivariate analysis of variance of the following explanatory variables: intervention group, age (</= to 80 compared to >80 years old), education background, marital status, gender and comorbidities. The domains of the participants’ self-rated experience were also assessed.The quality of life experience was similar at baseline in 2012 in both study groups. In 2014 the study group rated the quality of life (p<0.0001 respectively) and satisfaction with health (p=0.001 respectively) higher than the control group.Older people in care units need cultural activities as a necessary part of their care. The care provided in the care units does not put enough emphasis on this need. With individually tailored cultural activities set down in a cultural plan, care providers can ensure a better quality of life for older people.
A penalized method of alternating projections for weighted low-rank hankel matrix optimization
Authors: Shen Jian , Chen Jein-Shan , Qi Hou-Duo , Xiu Naihua 
Published: February 2022

AbstractWeighted low-rank Hankel matrix optimization has long been used to reconstruct contaminated signal or forecast missing values for time series of a wide class. The Method of Alternating Projections (MAP) (i.e., alternatively projecting to a low-rank matrix manifold and the Hankel matrix subspace) is a leading method. Despite its wide use, MAP has long been criticized of lacking convergence and of ignoring the weights used to reflect importance of the observed data. The most of known results are in a local sense. In particular, the latest research shows that MAP may converge at a linear rate provided that the initial point is close enough to a true solution and a transversality condition is satisfied. In this paper, we propose a globalized variant of MAP through a penalty approach. The proposed method inherits the favourable local properties of MAP and has the same computational complexity. Moreover, it is capable of handling a general weight matrix, is globally convergent, and enjoys local linear convergence rate provided that the cutting off singular values are significantly smaller than the kept ones. Furthermore, the new method also applies to complex data. Extensive numerical experiments demonstrate the efficiency of the proposed method against several popular variants of MAP.
Handling Qualities in Rotorcraft Conceptual Design
Authors: Zanoni Andrea , Gerosa Giacomo , Di Lallo Luigi , Masarati Pierangelo 
Published: February 2022

AbstractThis paper presents the development of a rotorcraft conceptual design tool able to incorporate handling qualities assessment at an early design stage. After a first conventional sizing, performed utilizing NASA’s NDARC software, a linearized model of the rotorcraft flight mechanics is built. The linear model is augmented by a simplified control system, designed according to structured $$H_{\infty }$$

H
∞

techniques, to determine augmentation requirements, rather than design the actual flight control system. ADS-33 Bandwidth and Phase-Delay standards are exploited to objectively assess the handling qualities of the current design and to drive an iterative redesign process aimed at enhancing the handling qualities ratings. The rotorcraft parameters resulting from the augmented sizing are subsequently used to automatically generate a real-time capable multibody model, which can be used for the subjective evaluation of its handling qualities via piloted flight simulation. The tool capabilities are demonstrated by designing a conventional lightweight helicopter of the class of the Airbus Helicopters BO105.
Critical review on microextraction techniques used in determination of histamine in food samples
Authors: Jayasinghe G. D. T. M. , Jinadasa B. K. K. K. , Pohl Pawel , Abdelkarim Aydi 
Published: February 2022

AbstractBiogenic amines are basic nitrogen compounds, which are formed due to the decarboxylation of amino acids or/and the amination and the transformation of aldehydes and ketones. Histamine is a heterocyclic biogenic amine present in a wide range of food products, including fish and fishery products, meat and their products, and other dairy products and beverages. The present review summarizes the innovative sample preparation methods for the extraction of histamine from various sample matrices. Both solid phase and liquid phase based microextraction techniques are used for extracting histamine, but the latter ones are the most popular. Currently, all the sample preparation techniques for the histamine extraction fulfil the demand of green chemistry standards. These sample preparation procedures are presented in the present review along with their special features, advantages and disadvantages.
Graphical Abstract
Single-Cell Transcriptomic Analysis Reveals the Cellular Heterogeneity of Mesenchymal Stem Cells
Authors: Zhang Chen , Han Xueshuai , Liu Jingkun , Chen Lei , Lei Ying , Chen Kunying , Si Jia , Wang Tian-yi , Zhou Hui , Zhao Xiaoyun , Zhang Xiaohui , An Yihua , Li Yueying , Wang Qian-Fei 
Published: February 2022

Abstract
Ex vivo-expanded mesenchymal stem cells (MSCs) have been demonstrated to be a heterogeneous mixture of cells exhibiting varying proliferative, multipotential, and immunomodulatory capacities. However, the exact characteristics of MSCs remain largely unknown. By single-cell RNA sequencing of 61,296 MSCs derived from bone marrow and Wharton’s jelly, we revealed five distinct subpopulations. The developmental trajectory of these five MSC subpopulations was mapped, revealing a differentiation path from stem-like active proliferative cells (APCs) to multipotent progenitor cells, followed by branching into two paths: 1) unipotent preadipocytes or 2) bipotent prechondro-osteoblasts that were subsequently differentiated into unipotent prechondrocytes. The stem-like APCs, expressing the perivascular mesodermal progenitor markers CSPG4/MCAM/NES, uniquely exhibited strong proliferation and stemness signatures. Remarkably, the prechondrocyte subpopulation specifically expressed immunomodulatory genes and was able to suppress activated CD3+ T cell proliferation in vitro, supporting the role of this population in immunoregulation. In summary, our analysis mapped the heterogeneous subpopulations of MSCs and identified two subpopulations with potential functions in self-renewal and immunoregulation. Our findings advance the definition of MSCs by identifying the specific functions of their heterogeneous cellular composition, allowing for more specific and effective MSC application through the purification of their functional subpopulations.
A permeameter for temperate ice: first results on permeability sensitivity to grain size
Authors: Fowler Jacob R. , Iverson Neal R. 
Published: February 2022

AbstractResults of ice-stream models that treat temperate ice deformation as a two-phase flow are sensitive to the ice permeability. We have constructed and begun using a custom, falling-head permeameter for measuring the permeability of temperate, polycrystalline ice. Chilled water is passed through an ice disk that is kept at the pressure-melting temperature while the rate of head decrease indicates the permeability. Fluorescein dye in the water allows water-vein geometry to be studied using fluorescence microscopy. Water flow over durations of seconds to hours is Darcian, and for grain diameter d increasing from 1.7 to 8.9 mm, average permeability decreases from 2 × 10−12 to 4 × 10−15 m2. In tests with dye on fine (d = 2 mm) and coarse (d = 7 mm) ice, average area-weighted vein radii are nearly equal, 41 and 34 μm, respectively. These average radii, if included in a theory slightly modified from Nye and Frank (1973), yield permeability values within a factor of 2.0 of best-fit values based on regression of the data. Permeability values depend on d−3.4, rather than d−2 as predicted by models if vein radii are considered independent of d. In future experiments, the dependence of permeability on liquid water content will be measured.
Animal-source foods as a suitable complementary food for improved physical growth in 6 to 24-month-old children in low- and middle-income countries: a systematic review and meta-analysis of randomised controlled trials
Authors: Asare Hannah , Rosi Alice , Faber Mieke , Smuts Cornelius M. , Ricci Cristian 
Published: February 2022

AbstractAlthough animal-source foods are suitable complementary food for child growth in low- and middle-income countries (LMICs), their efficacy is still under discussion. This systematic review and meta-analysis was done to investigate the suitability of animal-source foods intake on child physical growth in LMICs. A systematic literature search was done using electronic databases and scanning the reference list of included studies, previous meta-analysis and systematic reviews. Paper selection was based on the PICO (ST) criteria. Papers were selected if based on 6 to 24-month-old children, if they were randomised controlled trials evaluating the effect of complementary animal-based food supplementation of any natural origin, if reporting at least a measure of body size and published after 2000. The PRISMA guidelines for reporting systematic review was followed in the paper selection. Fourteen papers were included in the systematic review and eight were considered for the meta-analysis. Animal-based food supplementation resulted in a higher length-for-age LAZ and weight-for-age (WAZ) Z-scores compared with the control group with random effect size of 0·15 (95 % CI 0·02, 0·27) and 0·20 (95 % CI 0·03, 0·36), respectively. Results were confirmed after influence analyses, and publication bias resulted as negligible. An increased effect on LAZ and WAZ was observed when the food supplementation was based on egg with effect size of 0·31 (95 % CI = −0·03, 0·64) and 0·36 (95 % CI = −0·03, 0·75), respectively. Animal-source foods are a suitable complementary food to improve growth in 6 to 24-month-old children in LMICs.
Celebration or Critique? Performing Peer Gynt in the Heart of Norway
Authors: Harald Maagerø Lars 
Published: February 2022

Since 1988, Henrik Ibsen’s Peer Gynt has been performed outdoors in the summer at Gålå in Gudbrandsdalen in Norway. Peer Gynt is particularly connected to Norwegian national culture and identity, and this connection becomes particularly poignant at Gålå. The event focuses on giving the audience a celebratory national-romantic experience of the typical Norwegian landscape, traditional Norwegian culture, and Peer Gynt, the most Norwegian of all plays. The question is whether a production here necessarily has to be subjected to the celebratory atmosphere of the event or whether it is possible for a production in these circumstances to question the hegemonic, national-romantic ideology that pervades the event. This article uses Chantal Mouffe’s concept of ‘critical art’ and Louis Althusser’s reflections on the relationship between theatre and ideology to discuss this question in respect of how the productions at Gålå are marketed. The article argues that, whatever critical potential there is in performing Peer Gynt at Gålå, it can be found by directly staging and questioning the expectations that the event creates and that audience members bring with them. Sigrid Strøm Reibo employed such a strategy in her 2017 production, which is the focus of attention here. Lars Harald Maagerø is a scholar and director whose research is concerned with the political and cultural impact of contemporary theatre. His productions include A Midsummer Night’s Dream and Twelfth Night with Thesbiteatret in Norway, and Mozart’s Don Giovanni at Opera Østfold.
Behavioural responses of two penguin species to human presence at Barrientos Island, a popular tourist site in the Antarctic Peninsula region
Authors: Cajiao Daniela , Leung Yu-Fai , Tejedo Pablo , Barbosa Andrés , Reck Gunter , Benayas Javier 
Published: February 2022

AbstractVisitor Site Guidelines are the principal instruments guiding tourist activities and behaviour at intensively visited sites. These instruments attempt to minimize tourist impacts on Antarctic wildlife, including penguins. However, some recommendations still need to be reinforced by empirical research. Although penguins have enjoyed considerable research attention, a knowledge gap still exists regarding penguins' behavioural responses to realistic tourist activities, including talking sound, viewing distance and movement speed. To fill this gap, we conducted a series of experiments to simulate these activities on two penguin species breeding at an intensively visited site during the 2019–2020 season. We performed 106 replicates of passive and active human presence treatments. Responses varied between species, but active human presence consistently triggered significantly higher responses of strong vigilance behaviour. Our results reinforce Visitor Site Guidelines' recommendations of keeping quiet, moving slowly and increasing viewing distance if changes in behaviour are observed. We also recommend adopting a more conservative viewing distance in the early breeding season. Additional management-orientated empirical studies are needed, including on different species, sites and stages of the breeding season, as such results are valuable for strengthening tourism guidelines and assessing the efficacy of management measures under a post-COVID-19 scenario of increasing Antarctic tourism.
Childhood adversities and bipolar disorder: a neuroimaging focus
Authors: Zovetti Niccolò , Perlini Cinzia , Brambilla Paolo , Bellani Marcella 
Published: February 2022

Abstract
Early-life adverse events or childhood adversities (CAs) are stressors and harmful experiences severely impacting on a child's wellbeing and development. Examples of CAs include parental neglect, emotional and physical abuse and bullying. Even though the prevalence of CAs and their psychological effects in both healthy and psychiatric populations is established, only a paucity of studies have investigated the neurobiological firms associated with CAs in bipolar disorder (BD). In particular, the exact neural mechanisms and trajectories of biopsychosocial models integrating both environmental and genetic effects are still debated. Considering the potential impact of CAs on BD, including its clinical manifestations, we reviewed existing literature discussing the association between CAs and brain alterations in BD patients. Results showed that CAs are associated with volume alterations of several grey matter regions including the hippocampus, thalamus, amygdala and frontal cortex. A handful of studies suggest the presence of alterations in the corpus callosum and the pre-fronto-limbic connectivity at rest. Alterations in these regions of the brain of patients with BD are possibly due to the effect of stress produced by CAs, being hippocampus part of the hypothalamus–pituitary–adrenal axis and thalamus together with amygdala filtering sensory information and regulating emotional responses. However, results are mixed possibly due to the heterogeneity of methods and study design. Future neuroimaging studies disentangling between different types of CAs or differentiating between BD sub-types are needed in order to understand the link between CAs and BD.
Direct Observations of the Role of Lateral Advection of Sea Ice Meltwater in the Onset of Autumn Freeze Up
Authors: Crews Laura , Lee Craig M. , Rainville Luc , Thomson Jim 
Published: February 2022

AbstractIn seasonally ice‐free parts of the Arctic Ocean, autumn is characterized by heat loss from the upper ocean to the atmosphere and the onset of freeze up, in which first year sea ice begins to grow in open water areas. The timing of freeze up can be highly spatially variable, complicating efforts to provide accurate sea ice forecasting for marine operations. While melt season anomalies can be used to predict freeze up anomalies in some parts of the Arctic, this one‐dimensional view merits further examination in light of recent work demonstrating the importance of three‐dimensional flows in setting mixed layer properties in marginal ice zones. In this study, we show that horizontal advection of sea ice meltwater hastens freeze up in areas distant from the ice edge. We use nearly 800 temperature and salinity profiles along with satellite imagery collected in the central Beaufort Sea in autumn 2018 to document the roughly 100 km advection of a cold and fresh surface meltwater layer over several weeks. After the meltwater arrived, the mixed layer was cooler and shallower than the mixed layer in adjacent areas unaffected by the meltwater. The cooler and shallower meltwater‐influenced mixed layer promoted earlier ice formation. Within the meltwater‐affected area, advection was nearly as important as heat loss to the atmosphere for seasonally integrated mixed layer heat loss.