bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA-18a prevents senescence of mesenchymal stem
2 cells by targeting CTDSPL
Authors: Sun Bo 
Published: February 2022

Abstract
Stem cell therapy requires massive scale homogeneous stem cells under strictly qualification control. However, Prolonged ex vivo expansion impairs the biological functions and results in senescence of mesenchymal stem cells (MSCs). In this study, we revealed the up-regulations of CTDSPL in prolonged culture of MSCs derived from human umbilical cord (UCMSCs). Over-expression of CTDSPL resulted an enlarged morphology, up-regulation of p16 and accumulation of SA-β-gal of MSCs. The reduced phosphorylated RB suggested cell cycle arrest of MSCs. All these results implied CTDSPL induced premature senescence of MSCs. We further demonstrated miR-18a-5p was a putative regulator of CTDSPL by luciferase reporter assay. Inhibition of miR-18a-5p promoted the expression of CTDSPL and induced premature senescence of MSCs. Continuous overexpression of miR-18a-5p improved self-renewal of MSCs by reduced ROS level, increased expression of Oct4 and Nanog, promoted growth rate and differentiation capability. Hereby, we reported for the first time the dynamic interaction of miR-18a-5p and CTDSPL is crucial for stem cell senescence.
Potential Application of Dandelion (Taraxacum Mongolicum Hand.-Mazz.) on Broiler Chickens: Growth Performance, Expression of Tight Junction Protein and Mucin Genes, and Microbiota Composition in Ileum
Authors: Mao Jinju , Wang Yuan , Wang Wenwen , Duan Ting , Yin Na , Guo Tao , Guo Hui , Liu Na , An Xiaoping , Qi Jingwei 
Published: February 2022

Abstract
Background
Chinese herbs is becoming an exploitable alternative to the use of widely prohibited antibiotics in the poultry industry. We investigated the effects of dandelion on the growth performance and intestinal barrier function of broiler chickens. One-hundred and sixty 1-day-old Arbor Acres (AA) male broilers were randomly divided into four groups, with five replicates of eight birds each. The birds were fed a basal diet supplemented without (control group, [CON]) or with 500 (low dose [LD]) or 1000 (high dose [HD]) mg/kg dandelion or with 50 mg/kg chlortetracycline (CTC) for 42 days, including the starter phase (d 1 to 21) and the grower phase (d 22 to 42).
Results
During the starter phase, the average daily feed intake (ADFI) and feed/gain ratio (F/G) of LD was lower than CON, HD and CTC (P < 0.05). In the overall phase, the F/G of LD and HD both decreased (P < 0.05). At 21 days old, the gene expression of claudin, occludin-1, and mucin-1 of the LD and HD birds were significantly higher than that of CON and CTC birds (P < 0.05); the gene expression of claudin and occludin-1 was significantly higher in HD birds than in LD birds; and the gene expression of zonula occludens-1 (ZO-1) in HD birds was higher than that of CON and LD birds, but lower than that of CTC birds (all P < 0.05). At 42 days old, the gene expression of claudin, occludin-1, and mucin-1 of the LD birds was significantly higher than that of the other groups, whereas the gene expression of zo-1 was higher in the HD birds than in CON and LD birds (all P < 0.05). In the analysis of intestinal microbiota, decreased α-diversity was observed in 21-days-old HD birds, while LD and HD birds showed a significantly decreased relative abundance of Bacteroidata and Bacteroides at the level of phyla and genera (P < 0.05).
Conclusion
Overall, dietary dandelion supplementation can enhance growth performance by improving the intestinal barrier function of broilers. Therefore, dandelion represents a promising feed additive for broilers, although the optimal supplementation dose requires further study.
Evaluating the Performance of Small-Scale Irrigation Schemes in Sub Humid Ethiopian Highlands; a case study in Mugie and Fesas
Authors: Belay Agmasie M. , Assefa Tewodros , Belay Sisay A. , Yimam Abdu Y. 
Published: February 2022

Abstract
Background
This study was conducted to evaluate the performance of two small-scale irrigation (SSI) schemes in the sub-humid Ethiopian highlands. Water application at the farm level was monitored using partial flume and the average flow velocity along the main canal was measured using the current meter. The infiltration measurement was conducted at the head, middle, and tail of the scheme within the command area using a double-ring infiltrometer. Soil samples were collected using an auger up to 60cm depth. Climatic and agronomic data were collected from the nearby meteorological station and woreda agricultural office, respectively. CROPWAT was used to determine the water requirement of crops.
Results
The result showed that the average conveyance efficiency for the lined main canal was 90% and 92% for Mugie and Fesas SSI, respectively. The conveyance efficiency for the unlined main canal was in the order of 86% and 87% for Mugie and Fesas SSI. On the other hand, the average application efficiency and overall efficiency were found to be 56% and 51%, respectively, with storage efficiency of 50% for Mugie irrigation scheme. Similarly, the overall, application and storage efficiencies were found 50%, 54%, and 56%, respectively, for Fesas irrigation scheme. The relative irrigation and water supplies were 1.23 and 3.17, respectively, for Mugie SSI, while the supplies were 0.94 and 2.45, respectively, for Fesas SSI.
Conclusions
Conveyance efficiency for both Mugie and Fesas schemes was found below standard (95%) and overall efficacy was low as well (~50%). Failure of operating gates in the main canal, overtopping of water from the main canal due to canal siltation, and leakage through the main canals were the main causes for poor performance. Improvement measures are needed for both Mugie and Fesas irrigation schemes and institutional support is vital to improve and maintain the failure of infrastructures for better performance.
Necroptosis-related lncRNAs and Hepatocellular Carcinoma Undoubtedly Secret
Authors: Ning Jingyuan , Sun Keran , Fan Xiaoqing , Jia Keqi , Wang Xiaofeng , Wang Xiuli , Ma Cuiqing , Wei Lin 
Published: February 2022

Abstract
Background: The current study demonstrates that necroptosis is an important mechanism of carcinogenesis. However, the predictive value of necroptosis-associated long non-coding RNAs (LncRNAs) in hepatocellular carcinoma has not been demonstrated. The purpose of this study is to apply necroptosis-related lncRNAs to construct a predictive signature to predict the prognosis of hepatocellular carcinoma patients.Methods: The clinical and RNA-seq data were downloaded using The Cancer Genome Atlas (TCGA) database. Univariate and multivariate Cox analysis was used to screen out suitable necroptosis-related lncRNAs, and then predictive signature was constructed. The TCGA data were randomly divided into high- and low-risk groups, and the Kaplan-Meier method was used to analyze the overall survival (OS) of the two groups to verify the predictive signature. Finally, a prognostic correlation model for predicting disease-freesurvival （DFS）in hepatocellular carcinoma（HCC） was constructed and validated.Results: We had screened out 9 necroptosis-related lncRNAs (BACE1-AS, LINC01188, LUCAT1, PI3KCD-AS2, Z83851.1, AC009283.1, AC012360.2, AC015908.3, AC103760.1), which were used to construct a predictive signature, and draw the receiver operating characteristic (ROC) curve by the high- and low-risk group. It was found that the area under the curve (AUC) of risk score was 0.874, and the AUCs values of 1-,3-,and 5-years were 0.8, 0.759, 0.787. The TCGA data were randomly divided into two cohorts. In two cohorts, The OS of the high-risk groups were significantly lower than that of the low-risk groups, and the AUC of the ROC curves of the two cohorts were 0.851, 0.804, 0.802 and 0.735, 0.716, 0.76 at 1-, 3-, and 5-years. After quantifying immune cell subsets and related functions, it was found that the infiltration of active dendritic cells (aDCs), macrophages, mast cells, natural killer cells (NK), T regulatory cells (Tregs) were significantly different, and the expressions of immune checkpoints CD86, LAIR1, CTLA4, VTCN1, TNFRSF18, CD80, CD276, HHLA2, TNFSF4, TNFRSF8, TNFRSF4, TNFRSF9, LGALS9, HAVCR2 and TNFSF15 were also significantly different.Conclusion: This predictive signature can accurately predict the prognosis of hepatocellular carcinoma patients and provide guidance for the clinical treatment of hepatocellular carcinoma patients.
Cytotoxicity Evalution of Mannan Polysaccharide Purified from Mycobacterium Bovis for Use in Vaccine Formulation as an Adjuvant
Authors: Zahednia Sara , Madani Mahboobeh , Mohammadi-Sichani Maryam , Hassanzadeh Seyed Mehdi , Doroud Delaram 
Published: February 2022

Abstract
This study has been performed to determine cytotoxicity and immunogenicity of mannan containing vaccine formulations were assessed to evaluate the safety and efficacy. In this study, cytoxicity and immunogenicity were assessed to guarantee safety and efficacy. The cytoxicity of the extracted mannan on MDA-MB-231 cell lines and normal cell lines was evaluated employing MTT assay. To assess the in vivo toxicity, was performed conforming to Pharmacopoeia. The in vivo and in vitro cytoxicity assays, no significant difference was observed between the control and test groups in the terms of cell growth and animals survival. The cost-effectiveness of utilizing mannan purified from Mycobacterium bovis is an economically satisfactory method. The extracted mannan is also free of cytotoxicity.Revealed a safe/cost-effective while efficient tools as an adjuvant in vaccine studies.
A novel compound heterozygous BEST1 gene mutation in two siblings causing autosomal recessive bestrophinopathy
Authors: Haque Obaid Imtiyazul , Nabi Faisal , Ahmad Owais , Marques Joao Pedro 
Published: February 2022

Abstract
Purpose: To describe the clinical features, imaging characteristics, and genetic test results associated with a novel compound heterozygous mutation of BEST1 gene resulting in an autosomal recessive bestrophinopathy (ARB). Methods: Two siblings underwent a complete ophthalmic examination, including dilated fundus examination, fundus photography, fundus autofluorescence imaging, spectral-domain optical coherence tomography (OCT), fluorescein angiography (FA), electroretinography (ERG), and electrooculography (EOG). A clinical diagnosis of ARB was established based on multimodal retinal imaging. Subsequently, clinical exome sequencing, consisting of a panel of 6670 genes, was carried out to assess genetic alterations in the protein-coding region of the genome of the patients. Results: Two siblings (17-year-old female and 15-year-old male) presented with reduced visual acuity and bilateral symmetrical subretinal deposits of hyperautofluorescent materials in the posterior pole. Spectral-domain OCT demonstrated subretinal fluid accumulation. Clinical exome sequencing revealed two mutations in the protein-coding region of the BEST1 gene, including a novel mutation (R105S), creating aberrant BEST1 variants p.Glu35Lys (E35K) and p.Arg105Ser (R105S) expressing from the canonical BEST1 transcript. Conclusions: We have identified and described the phenotype of a novel BEST1 mutation (R105S) in a heterozygous state along with a previously reported mutation of BEST1 (E35K) causing autosomal recessive bestrophinopathy (ARB).
Coblation Adenotonsillar surgery: a game changer in decreasing postoperative complications while maintaining efficacy – an audit of 345 cases
Authors: Bitar Mohamad , Nazir Tanvir , Abd-Ul-Salam Hani 
Published: February 2022

Abstract
Purpose: To assess the safety and efficacy of coblation adenotonsillar surgery with emphasis on intra-capsular tonsillectomy (ICT) in children with both obstructive and infective indications.Methods: Children, 6 months to 18y of age, who underwent coblation adenotonsillar surgery by the senior author between Feb 2017 and Sep 2020, were included. We reviewed the demographic data, preoperative and postoperative symptoms and degree of obstruction, postoperative complications, the need for revision surgery.Results: We reviewed 345 patients; median age 4.5y (11 months – 16.3y, mean 5.2y). Most patients had snoring (94.2%), mouth breathing (92.8%), restless sleep (62.6%), and sleep disorder breathing (52.8%), 12.5% had recurrent tonsillitis. Median initial total symptoms score (TSS) was 4.5 (1-8; mean 4.1); 87.5% having 3 or more symptoms; this decreased postoperatively to a median of 0.0 (range 0 – 7; mean 0.2). Most patients underwent ICT (86.7%). Postoperative course was uneventful in most patients with median hospital stay of 1 day (0-3, mean1). Secondary bleeding was 1.7% [66.7% in extracapsular tonsillectomy (ECT)], none required admission or intervention. There was no tonsillar regrowth resulting in upper airway obstructive symptoms. None needed tonsillar revision surgery (only one patient needed revision adenoidectomy), at a median follow up of 27 months (range 9-47, mean 26.6). Conclusions: Coblation adenotonsillar surgery is a safe and effective procedure; ICT reduced the risk for postoperative bleeding to 0.7% and was self-limiting. The need for another tonsillar surgery was nil. We adopted ICT for both obstructive and infective indications based on our results and those of other published data.
Impact of Adjuvant Amniotic Membran Transplantation In Infectious Ulcerative Keratitis
Authors: Bulut Okyanus , Musayeva Gunel , selver ozlem barut 
Published: February 2022

Abstract
PURPOSE: To evaluate the impact of adjuvant human amniotic membrane transplantation(hAMT) in patients with infectious ulcerative keratitis on wound healing without waiting for the sterile phase of the infectious keratitis.METHODS: A retrospective cohort study based on medical records of patients who underwent hAMT procedure over a 2-year period (2020-2021) was conducted at Ege University Hospital, Turkey. Cryopreserved hAM was used. Best corrected visual acuity (BCVA) was measured with the Snellen chart. Detailed ophthalmological examination was performed at all visits. Treatment success was defined as complete healing including disappearance of corneal infiltrates, epithelial defect closure, reduction of anterior segment reaction.RESULTS: A total of 13 eyes of 12 patients who were diagnosed with ulcerative keratitis between 2020-2021. The mean age was 60.9 years and 66.6% of the patients were male. Average ulcer horizontal diameter was 9.7 (4.5–12) mm. A single layer human amniotic membrane (hAM) was transplanted 92.3% of eyes. Overlay technique was used in all patients for hAMT. The mean residence time of the amniotic membrane on the ocular surface was 13.3 (5-40) days. The mean time from the first presentation to the hAM transplantation was 9.8 (3-30) days. The average hospitalization time of all patients was 15 (3-46) days, pre- hAMT was 10.2 days and post hAMT was 4.8 days. The mean follow up time was 3 months. Recovery response was positive in 76.9%. Average healing time was 32.1 (6-57) days. CONCLUSIONS: hAMT was effective in the treatment of infectious ulcerative keratitis by accelerating wound healing and supporting the ocular surface biochemically and mechanically. Performing hAM transplantation at the early stages of the disease and prolonged residence of membrane on the ocular surface increase the success of the treatment and moreover abbreviates hospitalization time.
Medical Students Describe Their Wellness And How To Preserve It
Authors: Chatterjee Krishanu , Edmonds Victoria S. , Girardo Marlene E. , Vickers Kristin S , Hathaway Julie C , Stonnington Cynthia M. 
Published: February 2022

Abstract
Background: Despite widespread efforts to create wellness programming in medical schools, there is a paucity of literature examining students’ perception of wellness and perceptions of these programs. With the inaugural class at the Arizona campus of Mayo Clinic Alix School of Medicine (MCASOM-AZ), an opportunity arose to establish an empirically evaluated wellness curriculum that most inclusively and effectively enables medical students to flourish for years to come. The initial wellness offerings included mental health, academic success, and disability services, curriculum-embedded seminars, wellness committee driven programming, and student-proposed wellness activities. We aimed to improve the relevance and impact of medical school wellness curricula by soliciting in-depth and longitudinal perspectives of medical students themselves. As MCASOM-AZ opened in 2017, the student body at the time of study consisted of first- and second-year medical students. Methods: Employing a mixed methods analysis of qualitative and longitudinal quantitative data,first- and second-year students at a MCASOM-AZ were invited to respond to an anonymous, online year-long survey (baseline, six months and 12 months) during the 2018-2019 academic year and participate in a structured, in-depth and in-person, peer-to-peer interview about their conceptions of wellness and the MCASOM-AZ wellness curriculum and resources. Qualitative data was coded for themes using thematic analysis strategies by independent raters. Results: Nearly half of eligible students completed the baseline survey,1/3 completed all 3 time-points, and 1/5 participated in an in-depth interview. Participant age, gender, and year of school were representative of the larger student body. Although individual conceptions varied, Wellness was consistently highly valued. Family, Academic Performance, and Friends emerged as most important to well-being across time-points. Academic work arose as the largest barrier to wellness. Analysis of qualitative data revealed five themes. Despite individual differences in approaches to wellness, wellbeing was interrelated to the learning environment; mandatory wellness efforts that didn’t address the medical culture met with skepticism.Conclusions: Interview responses provided understanding and context by which to interpret questionnaire responses. Academics was critical to students’ identity and wellness, while also the largest barrier. Suggested curricular improvements include restructuring academic work, seamlessly integrating wellness within coursework, and offering optional individualized approaches.
Analysis of students’ positive emotion in green campus during COVID-19 pandemic in China
Authors: Liu Shaobo , Ji Yifeng , Li Jiang , Peng You , Li Zhitao , Lai Wenbo , Feng Tao 
Published: February 2022

Abstract
Green space in university campus is of paramount importance to students’ emotional and psychological restoration. Students’ positive emotion can be aroused when immersed in green space and naturalness. However, to what extent can perceived naturalness influence students’ positive emotion remains unclear, especially in the context of COVID-19 countermeasures. This study, therefore, attempts to investigate in-depth the nature and strength of the relationships between students’ positive emotion and their perceived naturalness, place attachment and landscape preference, which are potentially varying across universities in different social and environmental contexts and different restriction policies regarding the COVID-19 pandemic. A course of questionnaire-based surveys was administered in two university campuses in Heilongjiang and Hunan Provinces, China, resulting in 474 effective samples. Structural equation modeling was used to explore the hypothetical conceptual framework of latent variables and the indicators. The findings indicate that the higher students’ perceived naturalness results in the greater positive emotion. Students’ perceived naturalness in green spaces of campus has a positive effect on their place attachment and landscape preference. Moreover, the difference of mediate effects of place attachment and landscape preference were addressed which verifies the contextual influences.
Juvenile Heat Stress Tolerance in Triticum Durum - Aegilops Tauschii Derived Synthetics: a Way Forward for Wheat Improvement
Authors: Kaur Amandeep , Grewal Satvir Kaur , Kaur Sarabjit , Sharma Achla , Srivast Puja , Garg Monika , Wani Shabir Hussain , Chhuneja Parveen , Singh Kuldeep , Kaur Satinder 
Published: February 2022

Abstract
Background: Exponentially increasing population and everchanging climatic conditions are two major concerns for the global food security. Early sowing in second fortnight of October is an emerging trend with farmers in Indo Gangetic Plains to avoid the yield losses from terminal heat stress. This also benefits the use of residual soil moisture of rice crop, conserving about one irrigation. But most of the available wheat cultivars are not well adapted to early season sowing.Methods and Results: Two In-house developed SHWs, syn14128 and syn14170, were screened for juvenile heat stress. Seedling length, biochemical parameters and expression of amylase gene immediately after heat shock (HS) of 45°C for 12hr and 20hr and 24hr indicated a significantly lower MDA, H2O2 and higher free radical scavenging activities. Syn14170 reported higher total soluble sugar (TSS) under both HS periods, while syn14128 had a sustainable TSS content and amylase activity under HS as well as the recovery period. Conclusions: Both the SHWs had a lower oxidative damage along with high free radical scavenging under heat stress. The higher expression of amy4 along with sustainable TSS after heat stress in syn14128, indicated it as a potential source of juvenile heat stress tolerance. Variable response of SHWs to different biochemical parameters under heat stress opens the future perspectives to explore the enzymatic pathways underlying these responses.
Superoxide Dismutase Due To Occupational Metals Exposure To Nickel On Mutant P53 Dental Technicians
Authors: Berniyanti Titiek , Marhazlinda Marhazlinda , Palupi Retno , Dhinugrahini Bhetari Dwitya 
Published: February 2022

Abstract
Background: Nickel levels in dental technicians' blood serum are significantly linked to the generation of free radicals and catalyze the development of reactive oxygen species (ROS) (ROS). An increase in ROS causes oxidative stress and a p53 gene mutation. Superoxide dismutases (SODs) are important antioxidant enzymes that help the body fight oxidative stress. Purpose: The goal of this study is to see if dental technician occupational exposure to Ni contributes to the occurrence of p53 mutations and lower SOD activity in dental technicians. Methods: Blood samples were taken for 3 types of tests, namely Ni examination, with Atomic Absorbance Spectrophotometry (AAS), p53 mutant protein examination by ELISA method, and examination of SOD enzyme activity by Assay Method. Result: The concentration of nickel in dental technicians' blood samples is higher than in controls. Dental technician blood samples have lower levels of SOD than controls. Between dental technicians and controllers, there is a major difference. A negative connection exists between Ni and SOD, a positive correlation exists between Ni and p53mutant, and a negative correlation exists between SOD and p53mutant, according to the Spearman correlation test. Conclusion: A high nickel concentration in the blood serum of a dental worker raises the level of p53 mutants and lowers SOD activity, because p53 mutants have a negative dominant effect on wild p53 types, if wild-type p53 levels decline, p53 mutant levels may rise. Trial Registration: No: 149 / HRECC.FODM / VIII / 2017.