bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gender differences in the impact-factors of adolescent dissociative symptoms: a structural equation model approach
Authors: Cheng Qinglin , Deng Yuanyuan , Zhao Gang , Chen Junfang , Xie Li , Wang Le 
Published: August 2022

Abstract
Background
Few studies have investigated the gender-specific impact of adolescent dissociative symptoms (ADSs) in China. The purpose of this study was to examine gender differences in the impact factors of ADSs in Hangzhou City, China, as well as to investigate further the direct and interactive effects between ADSs and impact factors by constructing a structural equation model (SEM).
Methods
A school-based, two-center cross-sectional study was conducted in Hangzhou City, China. The sample comprised 2,160 adolescents aged 13–18 years who were randomly selected using a multi-phase, stratified, cluster sampling technique. We used a SEM to explore the direct and interactive effects between ADSs and influencing factors by gender stratification.
Results
A total of 1,916 participants were included in the two-center cross-sectional study. The male SEM model showed that the significant standardized paths (P < 0.05) were between psychological factors and environment factors, both positively related to ADSs (psychological factors and environment factors). In addition, a significant correlation was also observed between teacher-student conflict relationship and ADSs. The female SEM model indicated that there were the significant standardized paths between peer interpersonal interaction relationship and psychological factors, in which peer interpersonal interaction relationship was negatively correlated to ADSs, and psychological factors tended to correlate positively with ADSs. We also found that there was a correlation between peer interpersonal interaction relationship and hostility. Our findings indicated that the strongest effect was psychological factors for male (total standardized β = 0.365) or female ADSs (total standardized β = 0.674). The SEM model fit indices indicated that the proposed models fit the data for both male and female ADSs.
Conclusions
Our findings suggested that health resources and strategies could be allocated and performed rationally to enhance their utilization efficiency during ADSs screening and other public health actions. To reduce the risks of adolescent dissociative disorders and other mental disorders, we urgently need to develop gender-specific intervention strategies for ADSs.
Citizen science to determine sea turtles’ spatio-temporal parameters on a subtropical foraging ground
Authors: Neves-Ferreira Isabella , Mello-Fonseca Juliana , Ferreira Carlos Eduardo Leite 
Published: August 2022

Abstract
Sea turtles spend most of their life cycle in foraging grounds. Nevertheless, given the logistical ease, bulk of studies are carried out in nesting grounds. One strategy to gather information in foraging grounds is the use of cost-effective and non-invasive techniques that allow public participation. The present study aimed to use photographic-identification to investigate the spatio-temporal distribution of Chelonia mydas and Eretmochelys imbricata. Furthermore, we describe the occurrence of fibropapillomatosis. This work was carried out at subtropical rocky reefs of the Brazilian coast in Arraial do Cabo (22°57’S, 42°01’W), within a sustainable conservation unit. The images were obtained through social media screening, citizen science and intentional capture. A total of 641 photos (between 2006 and 2021) and 19 diving forms (between November 2019 and March 2020) were obtained. All diving forms presented at least one turtle. The photo-id identified 174 individuals of C. mydas, with 45 resighted individuals. E. imbricata had 32 individuals identified, and 7 individuals resighted. The median interval between the first and last individual sighting was 1.7 years for C. mydas and 2.4 years for E. imbricata. Fibropapillomatosis was only observed in C. mydas, with a prevalence of 13.99% and regression in 2 individuals (10.00%). The results indicated Arraial do Cabo as an important development area for sea turtles with resident individuals showing fidelity for at least 6 years. As the region accumulates anthropogenic impacts, it is critical to propose significant management measures to protect sea turtles from continuous and synergistic impacts, mainly on resident individuals.
Integrating Single-Cell RNA-seq to construct a Neutrophil prognostic model for predicting immune responses in non-small cell lung cancer
Authors: Pang Jianyu , Yu Qian , Chen Yongzhi , Yuan Hongjun , Sheng Miaomiao , Tang Wenru 
Published: August 2022

Abstract
Non-small cell lung cancer (NSCLC) is the most widely distributed tumor in the world, and its immunotherapy is not practical. Neutrophil is one of a tumor's most abundant immune cell groups. This research aimed to investigate the complex communication network in the immune microenvironment (TIME) of NSCLC tumors to clarify the interaction between immune cells and tumors and establish a prognostic risk model that can predict immune response and prognosis of patients by analyzing the characteristics of Neutrophil differentiation. Integrated single-cell RNA sequencing (scRNA-seq) data from NSCLC samples and Bulk RNA-seq were used for analysis. Twenty-eight main cell clusters were identified, and their interactions were clarified. Subsequently, four subsets of Neutrophils with different differentiation states were found, closely related to immune regulation and metabolic pathways. Based on the ratio of four housekeeping genes (ACTB, GAPDH, TFRC, TUBB), six Neutrophil differentiation-related genes (NDRGs) prognostic risk models, including MS4A7, CXCR2, CSRNP1, RETN, CD177, and LUCAT1, were constructed by Elastic Net and Multivariate Cox regression, and patients' total survival time and immunotherapy response were successfully predicted and validated in three large cohorts. Finally, the causes of the worse prognosis of NSCLC caused by six prognostic genes were explored, and the small molecular compounds targeted at the anti-tumor effect of prognostic genes were screened. This study clarifies the TIME regulation network in NSCLC and emphasizes the critical role of NDRGs in predicting the prognosis of patients with NSCLC and their potential response to immunotherapy, thus providing a promising therapeutic target for NSCLC.
Covered Transjugular Intrahepatic Portosystemic Shunt improves survival for variceal Bleeding in cirrhotic patients with hepatic venous pressure gradient≥16 mmHg: A a comparison study
Authors: Wang Xixuan , Yin Xiaochun , Gu Lihong , Guo Huiwen , Cheng Yang , Liu Yan , Xiao Jiangqiang , Wang Yi , Zhang Wei , Zou Xiaoping , Wang Lei , Zhang Ming , Zhuge Yuzheng , Zhang Feng 
Published: August 2022

AbstractObjectives:TIPS can decrease rebleeding in cirrhotic patients with variceal bleeding (VB), but it is controversial whether TIPS can improve survival. We aimed to assess whether TIPS improves survival in selected patients based on HVPG-related risk stratification in a long-term follow-up.Methods:Consecutive VB patients treated with endoscopic therapy+NSBBs or covered TIPS were retrospectively enrolled between January 2013 and December 2019. All patients had HVPG measurements by experienced personnel before therapy. Outpatient follow-up was performed regularly. The primary outcome was transplant-free survival. The secondary endpoints were rebleeding and OHE. Statistical analysis were performed.Results:According to the inclusion and exclusion criteria, 184 patients were analyzed (Mean age 55.27years ± 13.86, 107 males; 102 endoscopic therapy+NSBBs, 82 covered TIPS). Based on the HVPG-guided risk stratification, 70 patients had HVPGs lower than 16 mmHg, and 114 patients had HVPGs higher than 16 mmHg. The median follow-up time was 49.5 months. In the total cohort, the difference between the 2 therapies in terms of transplant-free survival was not statistically significant in the TIPS group (hazard ratio 0.6092, 95% CI 0.3546-1.046; p=0.0725). In the high-HVPG tier, transplant-free survival was higher in the TIPS group (hazard ratio 0.4385, 95% CI 0.2255-0.8525; p=0.0037). In the low-HVPG tier, transplant-free survival between the 2 treatments was similar without statistical significance (hazard ratio 0.8583, 95% CI 0.3301-0.232; p=0.7399). The difference in OHE between the two treatment groups was not statistically significant (P=0.0850; P=0.4791).Conclusions:TIPS can effectively improve transplant-free survival when HVPG is greater than 16 mmHg.
Achieve structural white inspired from quasi-ordered microstructures in Morpho theseus
Authors: Zhang Wang , Zhao Xinkun , Xiong Yuqin , Wang Wanlin , Zhang Di 
Published: August 2022

Abstract
As one of the most fascinating phenomena, structural whiteness in natural organisms serves as important functions in thermoregulation and mating. However, the architectures that cause the visible broadband reflection are often in quasi-disordered distribution, which hinders the systematic research of the color formation mechanism. Here, through numerical analysis, architectures in Morpho theseus scales are distributed in various tubular morphologies between tubular and gyroid structure. Then the mechanism of the structural white is discussed using the numerical model built with the combination of periodic numerical framework and random elements. Thermodynamic experiments indicate that the white scales can efficiently help reducing the temperature of butterfly wings under direct light beam. Our work provides a concise method to analyze the quasi-ordered structures. The methodology developed by this numerical model can facilitate deep understanding of performance improvement brought by these structural characteristics. Corresponding solutions can guide the design of nano-optical materials to achieve the efficient cooling, camouflage, and photothermal conversion system.
Identification of Coronavirus in Bats and Rodents From Northern and Central Argentina
Authors: Arteaga Franco Lucero , Miragaya Marcelo , Molina Nicolas , Mondino Mariela , Bracamonte Cesar , Capitelli Gabriel , Mundo Silvia , Bratanich Ana Cristina 
Published: August 2022

Abstract
Due to the present pandemic situation and the many animal species epidemiologically involved, a renewed interest has surged to investigate the coronavirus population circulating in wildlife, specially bats and rodents as potential reservoirs of new human pathogens. In Argentina, information about the viruses present in these mammals is very reduced. To investigate the presence of coronaviruses in this country, we obtained 457 samples from hematophagous, insectivorous, and frugivorous bats and rodents from two regions of Argentina. We report here the detection of alphacoronaviruses sequences in the three groups of bats as well as in rodents. Phylogenetic analysis showed the closest relationships to alphacoronaviruses from Brazil.
Molecular docking analysis and anti-apoptotic activity of Hypericum triquetrifolium Turra (St. John's-wort) in Cyclophosphamide-induced lung injury
Authors: Yıldız Songül Çetik , Keskin Cumali , Şahintürk Varol , Gür Bahri , Oğul Yasemin , CENGİZ Mustafa , Ayhanci Adnan 
Published: August 2022

AbstractThe present study aims to investigate the lung-protective and antiapoptotic effects ofHypericum triquetrifolium Turra(HTT) against cyclophosphamide (CP)-induced lung injury in rats. Thirty-five Sprague Dawley rats were categorized into 5 groups, each consisting of seven members. Phenolic acid and flavonoid contents of this plant were determined. The lung tissue samples cultivated from the rats were examined both histopathological and immunohistochemically for the apoptosis markers of Caspase-3, Bax, and Bcl-2. Histopathological results indicated that structural defects, bleeding areas, and edema had occurred in the lungs of the CP-Alone Group. Besides, Caspase-3 and Bax positivity of the lung cells had also increased while Bcl-2 positivity had decreased. On the other hand, in the HTT + CP Group, HTT was shown to have reversed the aforementioned changes positively. In addition, ourin-vivoresults were confirmed by thein-silicostudies. The changes that occurred in the binding of CP to the active-site amino acid residues of Caspase-3, Bax, and Bcl-2 upon the addition of Hyperoside besides the changes that occurred in their tendency to form hydrogen bonds were accounted for byin silicostudies. Based onin vivoandin silicoresults, HTT could be a strong protective candidate for CP-induced lung injury and apoptosis.
On the selection of a proper connection in describing the dynamics of constrained mechanical systems
Authors: Natsiavas Sotirios , Passas P. , Tzaferis K. 
Published: August 2022

Abstract
This study is focused on a critical issue related to the consistent application of Newton’s law of motion to a special but large class of mechanical systems, involving equality motion constraints. For these systems, it is advantageous to employ the general Analytical Dynamics framework, where their motion is represented by a curve on a non-flat configuration manifold. The geometric properties of this manifold, providing the information needed for setting up the equations of motion of the system examined, are fully determined by two mathematical entities. The first of them is the metric tensor, whose components at each point of the manifold are obtained by considering the kinetic energy of the system. The second geometric entity is known as the connection of the manifold. In dynamics, the components of the connection are established by using the set of the motion constraints imposed on the original system and provide the torsion and curvature properties of the manifold. Despite its critical role in the dynamics of constrained systems, the significance of the connection has not been investigated yet at a sufficient level in the current engineering literature. The main objective of the present work is to first provide a systematic way for selecting this geometric entity and then illustrate its role and importance in describing the dynamics of constrained systems.
Serum response factor reduces gene expression noise and confers cell state stability
Authors: Zhang Jian , Hu Xiao , Wu Qiao , Guo Shangqin 
Published: August 2022

Abstract
The role of serum response factor (Srf), a central mediator of actin dynamics and mechanical signaling, in cell identity regulation is debated to be either a cell identity stabilizer or destabilizer. We thus investigated the role of Srf in cell fate stability using mouse pluripotent stem cells, one of the very few cell types that can tolerate null Srf. Despite the fact that serum-containing cultures yield heterogeneous gene expression, deletion of Srf in mouse pluripotent stem cells leads to further exacerbated cell state heterogeneity. The exaggerated heterogeneity is not only detectible as increased lineage priming, but also as the 2C-like cell state. Thus, pluripotent cells explore more variety of cellular states in both directions of development surrounding naïve pluripotency, a behavior that is constrained by Srf. These results support that Srf functions as a cell state stabilizer, providing rationale for its functional perturbation in cell fate engineering and pathological intervention.
Antecedents of organizational commitment and its relationship with turnover intention: A study of business employees in pharmaceutical multi-national companies.
Authors: Can Hoang Nguyen Khanh , Nguyen Xuan Nhi 
Published: August 2022

AbstractBackground The high turnover rate in pharmaceuticals multi-national companies in Vietnam market in recent years has been received attention from not only human resources departments but also other commercial managers. The purpose of this research is to investigate the antecedents affecting organizational commitments and its relationship with turnover intention. Methods This research used a sample of sales and marketing employees (N = 357), both staff and manager roles, in twenty pharmaceuticals multi-national companies in Vietnam market. Mixed methods of qualitative and quantitative are utilized. Results Three of fifteen hypotheses are not supported which results in the fact that the employee voice does not impact on organizational commitment in general. Only two normative commitment and continuance commitment have the negative impacts to turnover intention. The career development and organizational justice have the biggest impact to normative commitment which also has the biggest impact to turnover intention. Conclusions The research results suggest the practical actions increasing the level of career development and organizational justice, in order to increase the level of normative commitment and to reduce the turnover intention. The present research is among the first ones to discover the antecedents affecting organizational commitment and its relationship with turnover intention, especially in the environment of pharmaceuticals multi-national companies in Vietnam.
Enhanced Neomycin antibiotic properties through proteolytic enzyme in Escherichia coli induced infection in broiler chicks
Authors: Uzair Muhammad Shahkar , Mushtaq Muhammad , Shah Muqader , Ullah Haq Aman , Sadique Umer , Khan Hamayun , Anwer Faisal , Ahmad Ijaz , Nawaz Noor-Ul-Ain 
Published: August 2022

Abstract

The study aimed to explore the beneficial aspect of Serratiopeptidase (proteolytic enzyme) combined with low profile antibiotic (Neomycin) in chickens against the E. coli infection at the finisher stage. Both in-vitro and in-vivo studies were carried out to evaluate the antimicrobial activities and potency of this combination. A total of one hundred and eighty (180) day-old chicks were randomly allotted to 6 groups i.e. G1 (negative control), G2 (positive control), G3 (standard antibiotic only), SN-1 (Serratiopeptidase @40g/L+ Neomycin@10mg/L), SN-2 (Serratiopeptidase @50mg/L+ Neomycin @20mg/L), and SN-3 (Serratiopeptidase @ 60mg/L+ Neomycin @30mg/L). Each group consisted of thirty birds with 5 replicate (6 birds/ replicate). On day 21, fresh inocula of E. coli (1x109cfu) were orally administered to all the groups except negative control. The results revealed that birds in SN-3 group showed significantly larger zone of inhibition, increased feed intake, weight gain and improved feed conversion ratio compared to standard and control groups. Moreover, reduced (p<0.05) mortality and morbidity index was found in SN-3 group among all the treated groups. Histopathological study revealed that SN-3 group had improved (p<0.05) tissue health and enhanced regenerative changes were observed. It was concluded, that use of serratiopeptidase combination with Neomycin has better impact on broiler chickens to enhance the antimicrobial effect of Neomycin against E. coli infection and to enhance production parameters.
Involvement of ACACA (acetyl-CoA carboxylase α) in the lung pre-metastatic niche formation in breast cancer by senescence phenotypic conversion in fibroblasts
Authors: Huang Yung-Chi , Hou Ming-Feng , Tsai Ying-Ming , Pan Yi-Chung , Tsai Pei-Hung , Lin Yi-Shiuan , Chang Chao-Yuan , Tsai Eing-Mei , Hsu Ya-Ling 
Published: August 2022

Abstract
BackgroundImpairment of metabolism is strongly associated with the development of cancer. However, the role of metabolic reprogramming in the remodeling of pre-metastatic niche (PMN), a key step in metastasis, is still unknown. We aimed to investigate the metabolic alternation during lung PMN formation.MethodsWe assessed the transcriptomes and lipidomics of lung of MMTV-PyVT mice by microarray and liquid chromatography-tandem mass spectrometry before lung metastasis. The validation of gene or protein expressions was performed by real-time quantitative polymerase chain reaction, immunoblot and immunohistochemistry. The lung fibroblasts were isolated from mice and then co-cultured with breast cancer to identify the influence of cancer on the change of lung fibroblasts in PMN.ResultsWe demonstrated changes in the lipid profile and several lipid metabolism genes in the lungs of breast cancer-bearing MMTV-PyVT mice before cancer spread. The expression of ACACA (acetyl-CoA carboxylase α) was downregulated in the lung fibroblasts, which contributed to changes in acetylation of protein’s lysine residues and the synthesis of fatty acid. The downregulation of ACACA in lung fibroblasts triggered a senescent and inflammatory phenotypic shift of lung fibroblasts in both in vivo and in vitro models. The senescence-associated secretory phenotype of lung fibroblasts enabled the recruitment of immunosuppressive granulocytic myeloid-derived suppressor cells into the lungs through the production of CXCL1 in the lungs. Knock-in of ACACA prevented lung metastasis in the MMTV-PyVT mouse model, further supporting that ACACA was involved in the remodeling of the lung PMN.ConclusionsTaken together, these data revealed a mechanism by which ACACA downregulation directs the formation of an immunosuppressive lung PMN in breast cancer.
Comparison of corrosion, tribocorrosion and antibacterial properties of silver coatings on Ti15Mo by magnetron sputtering
Authors: Meletlioglu Emrah , Sadeler Recep 
Published: August 2022

Abstract
The aim of this in vitro study was to evaluate the influence of + Ag coated conditions on the corrosion, tribocorrosion, antibacterial properties of Ti15Mo specimens. The mean wear volume loss of all test specimens after tribocorrosion test procedures were determined to use a noncontact 3D profilometer. The specimens hardness, roughness and microstructures were measured using the micro-hardness tester, surface profilometer, scanning electron microscopy (SEM) and Xray diffraction (XRD) analysis. The mean wear volume loss of 30 min + Ag coated Ti15Mo specimen was lower than the other specimens. In this study, correlations between the hardness, surface roughness and wear volume loss were found to be significant. The PVD coating process enhanced the antibacterial activity of Ti15Mo specimens owing mainly to the formation of the silver film on substrates