bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of renewable energy and storage technologies in sustainable development: simulation in the construction industry
Authors: Horzela-Miś Anna , Semrau Jakub 
Published: February 2025

IntroductionThe increasing demand for sustainable energy solutions has become crucial in addressing global environmental challenges such as climate change and resource depletion. As a significant energy consumer, the construction industry must explore renewable energy integration to enhance sustainability. This study investigates the role of photovoltaic (PV) systems and energy storage technologies in promoting sustainable energy use within a Polish construction manufacturing company.MethodsA 26-year simulation was conducted to analyze the implementation of a PV system in an industrial setting. The study assessed energy savings, cost efficiency, and environmental benefits by modeling energy consumption, production, and storage dynamics. The financial feasibility of the investment was evaluated using key performance indicators such as return on investment, payback period, and levelized cost of electricity.ResultsThe findings demonstrate that PV systems significantly reduce electricity expenses and reliance on grid power, achieving a payback period of approximately 9.4 years. The company has the potential to significantly reduce CO2 emissions, contributing to environmental sustainability. The simulation further revealed that PV adoption enhanced energy independence, covering 53.3% of the company’s energy needs while minimizing operational costs.DiscussionThe study highlights PV technology’s economic and environmental benefits in industrial applications. Energy storage integration optimizes energy utilization, increasing efficiency and financial viability. These findings provide valuable insights for construction companies considering renewable energy investments. The broader societal advantages, including CO2 reduction, improved public health, and regulatory compliance, underscore the importance of transitioning toward sustainable energy solutions.
The protective role of vitamin D in BNT162b2 vaccine-related acute myocarditis
Authors: Tsang Hing Wai , Chua Gilbert T. , Tung Keith Tsz Suen , Wong Rosa Sze Man , Tsao Sabrina Siu Ling , Wong Joshua Sung Chih , Tung Joanna Yuet Ling , Kwok Janette Siu Yin , Yam Jason Cheuk Sing , Chan Godfrey Chi Fung , To Kelvin Kai Wang , Wong Ian Chi Kei , Leung Wing Hang , Kwan Mike Yat Wah , Ip Patrick 
Published: February 2025

IntroductionVaccine-related myocarditis is recognized as a rare but important complication, especially after mass-scale mRNA COVID-19 vaccination. Knowledge regarding how to minimize the risk is limited. As NK cells can mediate acute myocarditis after mRNA COVID-19 vaccination and vitamin D may inhibit NK cells via cytokine modulation, we hypothesize that the myocarditis side effect is related to a hypovitaminosis D – mRNA vaccine – hypercytokinemia – NK cell axis, which is amendable to clinical intervention.MethodsBiochemical, immunophenotypic and genotyping assays were performed to examine vitamin D status and immune profiles in 60 patients who had BNT162b2 vaccine-related acute myocarditis.ResultsA high incidence of hypovitaminosis D (73.3%) was observed in these individuals with vaccine-related myocarditis, particularly in those presented with chest pain or intensive care unit (ICU) admission. Moreover, vitamin D level was negatively associated with peak serum cardiac troponin T level during vaccine-related myocarditis. Genotypically, the GC (vitamin D binding protein) rs4588T allele which encoded the GC2 isoform of vitamin D binding protein was a risk allele, whereas the GC1S isoform was protective. Mechanistically, hypovitaminosis D was associated with higher levels of cytokines pivotal for natural killer (NK) cells (particularly interleukin-1β (IL-1β), IL-12, Interferon-γ (IFN-γ), and IL-8) and higher percentage of CD69+ NK cells in blood, which in turn correlated with chest pain presentation.ConclusionThese data support the hypothesis that vitamin D plays a crucial role in mitigating mRNA vaccine-related myocarditis by modulating proinflammatory cytokine milieu and subsequent unfavorable NK cell activation, laying a groundwork for preventive and treatment strategies.
Unveiling macrophage dynamics and efferocytosis-related targets in diabetic kidney disease: insights from single-cell and bulk RNA-sequencing
Authors: Zhang Binshan , Wu Yunqi , Wang Zhongli , Gao Suhua , Liu Hongyan , Lin Yao , Yu Pei 
Published: February 2025

BackgroundChronic inflammation and immune imbalance mediated by macrophages are considered pivotal in diabetic kidney disease (DKD). The study aims to clarify the macrophage heterogeneity and phenotype dynamics, and pinpoint critical targets within efferocytosis in DKD.MethodsUtilizing early human DKD sequencing data, we computed the potential communication between leukocytes and renal intrinsic cells. Subsequently, we scrutinized the single-cell RNA sequencing (scRNA-seq) data from CD45-enriched immune cells, concentrating on the macrophage subsets in DKD. Pseudotime trajectory analysis was conducted to explore cell development. Differential expression genes (DEGs) from macrophage subgroups and bulk RNA-sequencing were used to identify shared hub genes. The NephroseqV5 platform was employed to evaluate the clinical significance, and the expression of key molecules was validated in DKD tissues.ResultsMacrophage infiltration rose in DKD, causing inflammation through the release of chemokines. As time progressed, the number of resident macrophages substantially dropped, with diminishing M1-like and increasing M2-like phenotypes relative to early stages. Further analysis pointed to the most enrichment of macrophage function is the phagosome. We overlapped the DEGs with efferocytosis-related genes and identified key genes, including CD36, ITGAM, and CX3CR1, which exhibited significant correlations with macrophages and T cells. The Nephroseq database revealed that they are associated with proteinuria and renal function. Consistent with the validation set, in vivo experiments verified elevated expression levels of key molecules.ConclusionsIn essence, our research elucidated the dynamics in macrophage subtype transitions. It emphasized three pivotal genes as critical modulators of macrophage efferocytosis in DKD, indicating their potential as innovative biomarkers and therapeutic targets.
Spatiotemporal distribution of ichthyoplankton and their relationship with environmental factors in the waters around the Yellow River estuary, China
Authors: Niu Mingxiang , Lu Feng , Zuo Tao , Wang Jun , Li Yongtao , Cheng Zhaolong , Sun Jianqiang 
Published: February 2025

IntroductionThe Yellow River estuary serves as a crucial convergence point between the Yellow River and the Bohai Sea and is a vital spawning, nursery, and feeding ground for a variety of fish species. Ichthyoplankton are essential for the replenishment and sustainable use of fishery resources. To gain insights into the distribution of fish in these early life stages and examine in depth the impacts of environmental factors on their presence and abundance in the waters around the Yellow River estuary, in this study, we analyzed the temporal and spatial distribution of ichthyoplankton and their correlation with environmental variables.MethodsData were gathered from horizontal-trawl surveys conducted between April and July of 2020, 2021, and 2023. In instances where no eggs or larvae were caught, a two-stage Generalized Additive Model (GAM) was employed to evaluate the influence of environmental factors on the presence/absence (P/A model) and abundance, given presence (Density model).ResultsThe results revealed that there were significant fluctuations in species richness both monthly and annually. Moreover, notable spatial disparities were identified in the distribution of abundance encompassing estuarine, settled, and migratory fish populations. The final model accounted for 38.69% and 39.80% of the deviance in the presence and abundance of ichthyoplankton, respectively. The two-stage GAM results highlighted that sea surface temperature (SST), chlorophyll a concentration, sea bottom salinity, and water depth were the dominant factors influencing the presence of ichthyoplankton. Moreover, a model incorporating SST, sea surface salinity, and distance to shore best described the abundance of ichthyoplankton when present.DiscussionOur findings contribute to understand the spatiotemporal dynamics of ichthyoplankton and their influencing factors in the waters around the Yellow River estuary, which have important implications for fisheries management.
Preliminary study on the participation of TLR9 on erythrocyte surface combined with mtDNA in the monitoring of infectious diseases
Authors: Xiao Ling , Xu Haixia , Liu Siwei , Cheng Zhanrui , Kong Yujie , Tian Li 
Published: February 2025

BackgroundTLR9 is typically found within cells and plays a crucial role in identifying pathogenic and self-DNA in chronic inflammation and immune complexes. Recent discoveries indicate its presence on the surface of human red blood cells, where it engages in immune regulation by binding to free mtDNA. The purpose of this study is to explore the role of TLR9 as a pattern recognition receptor combined with mtDNA in the monitoring of infectious diseases.MethodsTLR9 presence on the surface of red blood cells was assessed using flow cytometry in both healthy individuals and patients with bacterial infections. Subsequently, DNA bound to the red blood cell surface was extracted separately from both groups. The absolute quantification of mtDNA copy numbers within the extracted DNA was conducted using qPCR technology, followed by statistical analysis. Additionally, the correlation between mtDNA copy numbers bound to red blood cell surfaces in bacterial infection patients with varying CRP concentrations was examined using univariate linear regression.ResultIn healthy individuals, TLR9 expression on red blood cell surfaces averaged 8.81%. However, the average expression of TLR9 on red blood cell surfaces in patients with bacterial infection was 5.45%, which was lower than that in healthy people (p &lt; 0.001). Notably, both healthy individuals and infected patients exhibited mtDNA binding to red blood cell surfaces, with patients demonstrating a higher mtDNA copy number compared to healthy controls (p &lt; 0.001). Moreover, within the infected group, the copy numbers of mtDNA bound by red blood cells positively correlated with patient CRP concentrations (R2 = 0.715, p &lt; 0.001), indicative of an association between mtDNA copy numbers bound to red blood cell surfaces and infection severity.ConclusionThe elevation of erythrocyte-bound mtDNA during infection, coupled with its correlation with infection severity, suggests that monitoring the copy numbers of mtDNA bound to red blood cells via TLR9 could serve as a novel indicator for infection surveillance.
Isolated from Populus euphratica rhizosphere soil, and mining their metabolites
Authors: Zhang Jia-xin , Chen Yi-huang , Luo Xinrong , Zhang Li-li , Luo Xiao-xia 
Published: February 2025

IntroductionThe microbial community in desert ecosystems is a vital and highly active component. Streptomyces, one of the dominant genera within this community, exhibits significant capabilities in metabolic degradation and the synthesis of secondary metabolites.MethodsTo investigate the metabolic potential of Streptomyces desertis, two strains of Streptomyces were isolated from rhizosphere soil samples of Populus euphratica in the Taklimakan Desert during the initial phase of this study, TRM 70351T and TRM 70361T.ResultsThe identification of these strains as belonging to the genus Streptomyces was confirmed through 16S rRNA sequencing. All calculated Average Nucleotide Identity (ANI) values were below the 95% cut-off recommended for distinguishing distinct species, and the estimated digital DNA-DNA hybridization (dDDH) values were all below the suggested threshold of 70% for species delineation. Results from phylogenetic, genomic, phenotypic, and chemotaxonomic analyses confirmed that TRM 70351T and TRM 70361T represent two new species within the genus Streptomyces, designated as Streptomyces solitudinis sp. nov. and Streptomyces rubellus sp. nov. The type strain for Streptomyces solitudinis sp. nov. is TRM 70351T ( = CCTCC AA 2020049T = LMG 32404T), while the type strain for Streptomyces rubellus sp. nov. is TRM 70361T ( = CCTCC AA 2020043T = JCM 35793T). Notably, Among the identified gene clusters of TRM 70351T, cluster 12.1 was predicted to be the biosynthetic gene cluster responsible for producing the aminoglycoside compound streptomycin, exhibiting a similarity of 55%. In this study, HSQC-TOCSY was employed to detect the presence of aminoglycosides in fermentation medium No. 1, while LC-MS/MS was utilized to analyze the molecular fragments of neomycin and streptomycin in the alkaline aqueous phase sample of the fermentation product. The mixture was eluted using methanol and ammonia water in a 3:1 ratio, leading to the further separation of the compounds daidzein and Tridec-1-ene.DiscussionThis study has enhanced the species resources of Streptomyces deserticum and the diversity of aminoglycoside compound-producing bacteria. TRM 70351T exhibited unique metabolic potential, indicating that further studies could be conducted in the future.
Bibliometric analysis of global research on the clinical applications of aminoglycoside antibiotics: improving efficacy and decreasing risk
Authors: Zhao Tengxiang , Chen Nan , Zhang Mingyue , Lin Likai , Lin Bin , Fang Yuan , Hua Zhihui , Liang Chenyu 
Published: February 2025

BackgroundInfections by drug-resistant bacteria are a significant threat to human health worldwide although many drug-resistant bacteria are sensitive to aminoglycosides (AGs), an older class of antibiotics. AGs have played a significant role in clinical practice in recent years.MethodsPublications from 1 January 2013 to 31 December 2023 that described clinical research of AGs were identified by searching the Web of Science Core Collection Database. Visual presentations of different bibliometric networks were prepared using VOSviewer and CiteSpace.ResultsThere were 915 eligible publications and the annual number of publications increased over time. The United States had the most publications and was at the core of the cooperative network. Italy and Belgium had the highest quality publications, and many of the institutions with high yield and high research quality were in Australia. JA Roberts (University of Queensland, Australia) was the most productive author and was the author of many high-quality studies in cooperation with various other researchers. The majority of publications were in journals that focused on antibacterials, chemotherapy, and pharmacokinetics. Analysis of the most highly cited publications, references, and keywords, indicated that this research mainly focused on infections by drug-resistant bacteria, drug administration in vulnerable populations, safety, pharmacokinetics, combination therapy, and new methods of administration.ConclusionAGs have an increasingly important role in the treatment of infections by multidrug-resistant bacteria. Therapeutic drug monitoring should be performed in vulnerable populations, such as the elderly, children, and infants, to improve efficacy and reduce toxicity. Avoiding prolonged dosing cycles and refraining from using AGs in patients with the m.1555 A &gt; G gene variant can significantly mitigate the risk of ototoxicity. Future studies should examine the pharmacokinetic and pharmacodynamic targets of AGs and assess the efficacy and safety of administration by inhalation to improve efficacy and decrease risk.
Research hotspots and development trends of Brucellar spondylitis in the past 30 years: a bibliometric analysis
Authors: Wu Jinyu , Yuan Hongxia , Chen Hongchao 
Published: February 2025

ObjectiveWith the growing emphasis on global health issues, Brucellar spondylitis, a serious complication of brucellosis, has received increasing attention from researchers. This study employed bibliometric analysis to visually illustrate the scientific advancements and research trends in the field of Brucellar spondylitis research, providing a scientific basis for public health prevention and control strategies.MethodThe data were sourced from the Web of Science Core Collection database, covering the period from January 1, 1984, to December 31, 2023. Network analyses of countries, institutions, authors, and keyword collaborations, as well as co-citation analyses of journals and references, were conducted using CiteSpace software. VOSviewer software was used to analyze the co-occurrence and hotspots of keywords.ResultA total of 246 relevant documents were retrieved, comprising 221 articles and 25 review articles. From 1984 to 2023, the number of research publications exhibited a generally fluctuating upward trend. Turkey and China emerged as the primary contributing countries in this field, with Xinjiang Medical University identified as the most productive research institution. Additionally, Juan D. Colmenero is recognized as the author with the highest number of published articles and citations. “Clinical Infectious Diseases” is regarded as the most influential journal in this domain. Among the 404 keywords analyzed by CiteSpace, the top 10 high-frequency keywords included spondylitis, complication, melitensis, osteoarticular complication, diagnosis, infection, clinical characteristics, epidural abscess, arthritis, and feature. A total of 14 clusters were formed. In the burst analysis of the top 15 keywords, “instrumentation” exhibited the highest burst intensity, while “arthritis” demonstrated the longest burst duration. Emerging keywords include “experience,” “ankylosing spondylitis,” “China,” and “instrumentation.”ConclusionThis study is the first bibliometric analysis in the field of Brucellar spondylitis, which revealed that the research hotspots in this field included the clinical characteristics of the disease, the management of complications, and treatment strategies. The development trend may involve enhancements in early diagnostic methods and advancements in surgical instruments. This study serves as a valuable reference for future research directions.
Alterations in brain function in patients with post-stroke cognitive impairment: a resting-state functional magnetic resonance imaging study
Authors: Han Kaiyue , Dong Linghui , Liao Xingxing , Long Junzi , Chen Jiarou , Lu Haitao , Zhang Hao 
Published: February 2025

BackgroundCognitive impairment is a common dysfunction following stroke, significantly affecting patients’ quality of life. Studies suggest that post-stroke cognitive impairment (PSCI) may be related to neural activity in specific brain regions. However, the neural mechanisms remain to be further explored. This study aimed to investigate the alterations in brain function in patients with PSCI.MethodsThis was a case–control study. Thirty patients with PSCI, thirty with non-PSCI (NPSCI), and thirty age- and gender-matched healthy controls (HCs) were selected in a 1:1:1 ratio. Resting-state functional magnetic resonance imaging (rs-fMRI) were acquired from all participants to study the potential neural mechanisms of PSCI patients by comparing the differences in fractional amplitude of low-frequency fluctuation (fALFF), Kendall’s coefficient of concordance-regional homogeneity (KCC-ReHo), and seed-based functional connectivity (FC). Additionally, the Montreal Cognitive Assessment (MoCA) scores of PSCI patients were collected, and Pearson correlation was used to analyze the correlation between functional indicators and cognitive performance in PSCI patients.ResultsfALFF analysis revealed that the PSCI group had decreased zfALFF values in the left caudate, right inferior temporal gyrus (ITG), anterior cingulate cortex (ACC), left putamen, and left superior temporal gyrus. In contrast, increased zfALFF values were observed in the right Cerebellum_6. KCC-ReHo analysis indicated that the PSCI group had decreased SzKCC-ReHo values in the right middle frontal gyrus (MFG) and left postcentral lobe, while increased SzKCC-ReHo values in the left cerebellum_ crus 1, and left cerebellum_4–5. Furthermore, seed-based FC analysis revealed decreased zFC values between brain regions in the PSCI group, especially between the angular gyrus and precuneus. Additionally, correlation analysis showed that the zfALFF value of ACC was positively correlated with MoCA scores in the PSCI group.ConclusionThis study demonstrated significant changes in the spontaneous neural activity intensity, regional homogeneity, and FC of multiple cognition-related brain regions in PSCI patients, shedding light on the underlying neural mechanisms of brain function in PSCI.
Relationship between Body Roundness Index and cognitive impairment in middle-aged and older adults: a population-based cross-sectional study
Authors: Guo Dandan , Li Tao , Yang Qingchao , Yang Chenlu , Yang Yang , Liu Fuchen , Ma Jun , Tu Jun , Ning Xianjia , Wang Jinghua , Song Chengyuan , Liu Yiming 
Published: February 2025

BackgroundCognitive impairment is a growing public health concern, particularly in aging populations. Obesity, as measured by various indices, has been linked to cognitive decline, but the relationship between Body Roundness Index (BRI) and cognitive impairment remains unclear. This study aims to evaluate the association between BRI and cognitive impairment in a rural, low-income, low-education population in China and to determine if BRI can be used as an independent predictor of cognitive decline.MethodsThis cross-sectional study included the participants aged 35–95 years from rural Tianjin, China. The mean age of the study population was 64.35 ± 7.58 years. Data were collected through face-to-face interviews, physical examinations, and laboratory tests. Cognitive function was assessed using the Mini-Mental State Examination (MMSE), and BRI was calculated and grouped into quartiles. Univariate and multivariate logistic regression analyses were performed to examine the relationship between BRI and cognitive impairment. Subgroup analyses were conducted to explore interactions between BRI, age, gender, and hypertension. The dose–response relationship was analyzed using restricted cubic spline models.ResultsOf the participants, 36.5% had cognitive impairment. Multivariate analysis showed that women, individuals aged 65 and over, and those with hypertension had a higher risk of cognitive impairment. Participants in the second quartile of BRI had a 31% lower risk of cognitive impairment compared to the first quartile (OR: 0.69, 95% CI: 0.51–0.94, p = 0.017). Subgroup analysis revealed that BRI was significantly associated with cognitive impairment in individuals under 65, but not in older participants. The dose–response relationship between BRI and MMSE score showed an inverted U-shaped curve, with the weakest association observed around a BRI of 4.49.ConclusionBody Roundness Index, in conjunction with age, gender, and hypertension, can serve as a useful predictor of cognitive impairment, particularly in younger populations. Early identification of individuals at risk through BRI may facilitate timely interventions, reducing the burden of cognitive decline on patients and healthcare systems.