bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of the COVID-19 pandemic on harm reduction services in Catalonia: the experience of people who use drugs and harm reduction professionals
Authors: Bosch-Arís Mar , Gasulla Laia , de Gispert Teresa , Segura Lidia , Colom Joan 
Published: October 2022

Abstract
Background
Harm reduction services and professionals have had to reorganise and adapt to COVID-19 prevention measures while still ensuring health and social services for people who use drugs (PUD).

Objective
To assess the impact of the COVID-19 pandemic on PUD and on the professionals who provide harm reduction services.

Methods
A qualitative, exploratory, multicentre design was used. Two focus groups were held with harm reduction professionals, and 40 individual semi-structured interviews were undertaken with PUD in various harm reduction services in Catalonia. Interviews and focus group discussions were transcribed and analysed using thematic content analysis.

Results
Harm reduction services adapted to the pandemic situation by employing methods such as reducing opening hours and closing drop in areas, along with health protection measures such as access control, which in turn led to stress among both professionals and service users. Despite the changes implemented, PUD continued to have access to sterile drug use equipment and methadone treatment. In addition, those who were not in treatment were able to access it rapidly. Regarding their emotional state, the PUD reported that it was worse during the pandemic than before the lockdown, with women affected to a greater extent than men. The harm reduction professionals reported difficulties in managing service users’ compliance with the security measures at the beginning of the lockdown and having had to focus primarily on providing food and shelter for the PUD.

Conclusions
It is important to keep PUD in mind and maintain a harm reduction perspective when implementing confinement measures in situations such as those experienced during the COVID pandemic. Guaranteeing that PUD have their basic needs such as food, hygiene and shelter covered is key.
Concanavalin A as a promising lectin-based anti-cancer agent: the molecular mechanisms and therapeutic potential
Authors: Huldani Huldani , Rashid Ahmed Ibraheem , Turaev Khikmatulla Negmatovich , Opulencia Maria Jade Catalan , Abdelbasset Walid Kamal , Bokov Dmitry Olegovich , Mustafa Yasser Fakri , Al-Gazally Moaed E. , Hammid Ali Thaeer , Kadhim Mustafa M. , Ahmadi Seyed Hossein 
Published: October 2022

AbstractConcanavalin A (ConA), the most studied plant lectin, has been known as a potent anti-neoplastic agent for a long time. Since initial reports on its capacity to kill cancer cells, much attention has been devoted to unveiling the lectin's exact molecular mechanism. It has been revealed that ConA can bind to several receptors on cancerous and normal cells and modulate the related signaling cascades. The most studied host receptor for ConA is MT1-MMP, responsible for most of the lectin's modulations, ranging from activating immune cells to killing tumor cells. In this study, in addition to studying the effect of ConA on signaling and immune cell function, we will focus on the most up-to-date advancements that unraveled the molecular mechanisms by which ConA can induce autophagy and apoptosis in various cancer cell types, where it has been found that P73 and JAK/STAT3 are the leading players. Moreover, we further discuss the main signaling molecules causing liver injury as the most significant side effect of the lectin injection. Altogether, these findings may shed light on the complex signaling pathways controlling the diverse responses created via ConA treatment, thereby modulating these complex networks to create more potent lectin-based cancer therapy.
Single-cell RNA sequencing reveals a landscape and targeted treatment of ferroptosis in retinal ischemia/reperfusion injury
Authors: Li Yangyang , Wen Yuwen , Liu Xiuxing , Li Zhuang , Lin Bingying , Deng Caibin , Yu Ziyu , Zhu Yingting , Zhao Ling , Su Wenru , Zhuo Yehong 
Published: October 2022

Abstract
Background
The aim of this study was to establish a complete retinal cell atlas of ischemia–reperfusion injury by single-cell RNA sequencing, and to explore the underlying mechanism of retinal ischemia–reperfusion injury in mice.

Methods
Single-cell RNA sequencing was used to evaluate changes in the mouse retinal ischemia reperfusion model. In vivo and in vitro experiments were performed to verify the protective effect of inhibiting ferroptosis in retinal ischemia–reperfusion injury.

Results
After ischemia–reperfusion injury, retinal cells were significantly reduced, accompanied by the activation of myeloid and a large amount of blood-derived immune cell infiltration. The IFNG, MAPK and NFKB signaling pathways in retinal neuronal cells, together with the TNF signaling pathway in myeloid give rise to a strong inflammatory response in the I/R state. Besides, the expression of genes implicating iron metabolism, oxidative stress and multiple programed cell death pathways have changed in cell subtypes described above. Especially the ferroptosis-related genes and blocking this process could apparently alleviate the inflammatory immune responses and enhance retinal ganglion cells survival.

Conclusions
We established a comprehensive landscape of mouse retinal ischemia–reperfusion injury at the single-cell level, revealing the important role of ferroptosis during this injury, and targeted inhibition of ferroptosis can effectively protect retinal structure and function.
Targeting ERRα promotes cytotoxic effects against acute myeloid leukemia through suppressing mitochondrial oxidative phosphorylation
Authors: Seo Wonhyoung , Yoo Seungyeul , Zhong Yi , Lee Sang-Hee , Woo Soo-Yeon , Choi Hee-Seon , Won Minho , Roh Taylor , Jeon Sang Min , Kim Kyeong Tae , Silwal Prashanta , Lee Min Joung , Heo Jun Young , Lawlor Nathan , Kim Sup , Lee Dongjun , Kim Jin-Man , Song Ik-Chan , Zhu Jun , Jo Eun-Kyeong 
Published: October 2022

AbstractAcute myeloid leukemia (AML) is an aggressive blood cancer with poor clinical outcomes. Emerging data suggest that mitochondrial oxidative phosphorylation (mtOXPHOS) plays a significant role in AML tumorigenesis, progression, and resistance to chemotherapies. However, how the mtOXPHOS is regulated in AML cells is not well understood. In this study, we investigated the oncogenic functions of ERRα in AML by combining in silico, in vitro, and in vivo analyses and showed ERRα is a key regulator of mtOXPHOS in AML cells. The increased ERRα level was associated with worse clinical outcomes of AML patients. Single cell RNA-Seq analysis of human primary AML cells indicated that ERRα-expressing cancer cells had significantly higher mtOXPHOS enrichment scores. Blockade of ERRα by pharmacologic inhibitor (XCT-790) or gene silencing suppressed mtOXPHOS and increased anti-leukemic effects in vitro and in xenograft mouse models.
Acute perchloroethylene intoxication in an elderly woman: a case report
Authors: Tarditi Alessandro , Montalbano Leda , Spina Stefano , Marrazzo Francesco , Casella Giampaolo , Schenardi Paolo , Conti Tommaso , Angeli Ilaria , Minoli Mauro , Fumagalli Roberto , Langer Thomas 
Published: October 2022

Abstract
Background
Perchloroethylene is a colorless, strong-smelling substance commonly used for dry cleaning. Liver and kidney toxicities and carcinogenicity are well-known occupational hazards caused by chronic perchloroethylene exposure. Acute intoxication by ingestion of nondiluted perchloroethylene is rare in the adult population owing to its strong smell and taste. Very few data are available to physicians managing patients in this situation.

Case presentation
An 89-year-old Caucasian woman accidentally drank perchloroethylene while visiting her laundry, leading to a coma within a few minutes. The poison control center provided little information about perchloroethylene toxicity after ingestion, including an estimated long biological half-life (144 hour) and detrimental effects to liver and kidneys. A long intensive care unit stay was thus expected, potentially leading to several complications. After intubation, transitory hypoxemia appeared and rapidly resolved, while mild hemodynamic instability was managed with fluid resuscitation and anti-arrhythmic drugs. Twelve hours after perchloroethylene ingestion, the patient suddenly woke up and self-extubated. Less than 24 hours after ingestion, she was discharged from the intensive care unit, and 4 days later she was discharged home.

Conclusion
The patient drank perchloroethylene from a bottle, which prevented her from smelling it, and owing to its taste, only a small sip was likely drunk. However, a much larger intake was presumed, given her rapid and profound central nervous system depression. This case was challenging owing to the paucity of information available regarding acute perchloroethylene ingestion and the duration and magnitude of its effect. The present report will hopefully be of support for clinicians managing patients with this rare acute intoxication.
Screening for prostate cancer: protocol for updating multiple systematic reviews to inform a Canadian Task Force on Preventive Health Care guideline update
Authors: Bennett Alexandria , Beck Andrew , Shaver Nicole , Grad Roland , LeBlanc Allana , Limburg Heather , Gray Casey , Abou-Setta Ahmed , Klarenbach Scott , Persaud Navindra , Thériault Guylène , Thombs Brett D. , Todd Keith J. , Bell Neil , Dahm Philipp , Loblaw Andrew , Del Giudice Lisa , Yao Xiaomei , Skidmore Becky , Rolland-Harris Elizabeth , Brouwers Melissa , Little Julian , Moher David 
Published: October 2022

Abstract
Purpose
To inform updated recommendations by the Canadian Task Force on Preventive Health Care on screening for prostate cancer in adults aged 18 years and older in primary care. This protocol outlines the planned scope and methods for a series of systematic reviews.

Methods
Updates of two systematic reviews and a de novo review will be conducted to synthesize the evidence on the benefits and harms of screening for prostate cancer with a prostate-specific antigen (PSA) and/or digital rectal examination (DRE) (with or without additional information) and patient values and preferences. Outcomes for the benefits of screening include reduced prostate cancer mortality, all-cause mortality, and incidence of metastatic prostate cancer. Outcomes for the harms of screening include false-positive screening tests, overdiagnosis, complications due to biopsy, and complications of treatment including incontinence (urinary or bowel), and erectile dysfunction. The quality of life or functioning (overall and disease-specific) and psychological effects outcomes are considered as a possible benefit or harm. Outcomes for the values and preferences review include quantitative or qualitative information regarding the choice to screen or intention to undergo screening. For the reviews on benefits or harms, we will search for randomized controlled trials, quasi-randomized, and controlled studies in MEDLINE, Embase, and the Cochrane Central Register of Controlled Trials. For the review on values and preferences, we will search for experimental or observational studies in MEDLINE, Embase, and PsycInfo. For all reviews, we will also search websites of relevant organizations, gray literature, and reference lists of included studies. Title and abstract screening, full-text review, data extraction, and risk of bias assessments will be completed independently by pairs of reviewers with any disagreements resolved by consensus or by consulting with a third reviewer. The GRADE (Grading of Recommendations Assessment, Development and Evaluation) approach will be used to assess the certainty of the evidence for each outcome.

Discussion
The series of systematic reviews will be used by the Canadian Task Force on Preventive Health Care to update their 2014 guideline on screening for prostate cancer in adults aged 18 years and older.
Systematic review registration
This review has been registered with PROSPERO (CRD42022314407) and is available on the Open Science Framework (osf.io/dm32k).
DUSP5 and PHLDA1 mutations in mature cystic teratomas of the ovary identified on whole-exome sequencing may explain teratoma characteristics
Authors: Wang Wen-Chung , Lai Yen-Chein 
Published: October 2022

Abstract
Background
Mature cystic teratomas of the ovary are the most common type of germ cell tumor, comprising 33% of ovarian tumors. Studying these tumors may result in a better understanding of their stepwise developmental processes and molecular bases and provide useful information for the development of tissue-engineering technologies.

Methods
In the present study, 9 mature cystic teratomas of the ovary were analyzed by whole-exome sequencing and the results were compared with the Catalogue of Somatic Mutations in Cancer and dbSNP databases.

Results
Mutations were validated in 15 genes with alterations in all 9 (100%) samples and changes in protein coding. The top 10 mutated genes were FLG, MUC17, MUC5B, RP1L1, NBPF1, GOLGA6L2, SLC29A3, SGK223, PTGFRN, and FAM186A. Moreover, 7 variants in exons with changes in protein coding are likely of importance in the development of mature cystic teratomas of the ovary, namely PTGFRN, DUSP5, MPP2, PHLDA1, PRR21, GOLGA6L2, and KRTAP4-2.

Conclusions
These genetic alterations may play an important etiological role in teratoma formation. Moreover, novel mutations in DUSP5 and PHLDA1 genes found on whole-exome sequencing may help to explain the characteristics of teratomas.
Non-clinical safety evaluation of salvianolic acid A: acute, 4-week intravenous toxicities and genotoxicity evaluations
Authors: Yang Ming-Yan , Song Ze-Yu , Gan Hai-Lin , Zheng Mei-Hua , Liu Qian , Meng Xiang-Ting , Pan Tao , Li Zhen-Yuan , Peng Ruo-Xuan , Liu Ke , Fan Hua-Ying 
Published: October 2022

Abstract
Background
Toxicological problem associated with herbal medicine is a significant public health problem. Hence, it is necessary to elaborate on the safety of herbal medicine. Salvianolic acid A (SAA) is a major active compound isolated from Danshen, a popular herbal drug and medicinal food plant in China. The aim of the present study was to explore the toxicological profile of SAA.

Methods
The acute toxicity studies were performed in mice and Beagle dogs with single administration with SAA. A 4-week subchronic toxicity was test in dogs. SAA was intravenously administered at doses of 20, 80 and 300 mg/kg. Clinical observation, laboratory testing and necropsy and histopathological examination were performed. The genotoxic potential of SAA was evaluated by 2 types of genotoxicity tests: a reverse mutation test in bacteria and bone marrow micronucleus test in mice.

Results
In acute toxicities, the LD50 of SAA is 1161.2 mg/kg in mice. The minimum lethal dose (MLD) and maximal non-lethal dose (MNLD) of SAA were 682 mg/kg and 455 mg/kg in dogs, respectively. The approximate lethal dose range was 455–682 mg/kg. In the study of 4-week repeated-dose toxicity in dogs, focal necrosis in liver and renal tubular epithelial cell, the decrease in relative thymus weight, as well as abnormal changes in biochemical parameters, were observed in SAA 80 or 300 mg/kg group. The no observed adverse effect level (NOAEL) of SAA was 20 mg/kg. Thymus, liver and kidneys were the toxic targets. These toxic effects were transient and reversible. These results indicated that it should note examination of liver and kidney function during the administration of SAA in clinic. Furthermore, SAA had no mutagenic effect at any tested doses.

Conclusion
These results provide new toxicological information of SAA for its clinical application and functional food consumption.
Fractures of limbs specific to children: the experience of a Senegalese tertiary hospital
Authors: Zeng Florent Tshibwid A , Fall Mbaye , Mbaye Papa Alassane , Gueye Doudou , Bisseyou Audrey Kongo , Seck Ndeye Fatou , Camara Souleymane , Wellé Ibrahima Bocar , Diedhiou Youssouph , Ndoye Ndeye Aby , Sagna Aloïse , Ndour Oumar , Ngom Gabriel 
Published: October 2022

Abstract
Introduction
Fractures specific to the pediatric age group represent a particular injury due to their pattern, diagnosis, management, and outcomes. In sub-Saharan Africa, studies on this particular injury are scarce. This study reports sociodemographic, diagnostic, and therapeutic aspects and outcomes of these fractures.

Methods
We conducted a descriptive cross sectional study at the pediatric surgery department of Aristide Le Dantec University Teaching Hospital in Dakar, Senegal, from January 2012 to December 2015.

Results
A fracture specific to children was diagnosed in 180, of whom 47.7% were school-aged, with 59.4% of males. The mean time from injury to the attendance of our department was 48.2 h. Domestic accidents occurred in 51.1%. A total of 243 fractures occurred, mainly on the upper limbs (75.3%), as the most affected bones were the radius (47.3%), ulna (22.6%), and tibia (13.6%). Greenstick fractures represented 46.9%, buckle fractures, 42.8%, plastic deformations 9%, and subperiosteal fractures 0.7%. In all patients, management was orthopedic, with associated analgesic treatment. No sequel nor other complication was registered.

Conclusion
Fractures proper to the child are a particular entity in children’s trauma, frequently happening in boys, with greenstick and buckle fractures being the most common. Their treatment is exclusively orthopedic, with excellent outcomes.
Fault detection using a convolutional neural network trained with point-spread function-convolution-based samples
Authors: Jing Jiankun , Yan Zhe , Zhang Zheng , Gu Hanming , Han Bingkai 
Published: October 2022

Automatic fault detection in seismic images using deep learning-based methods has attracted great interest in recent years. Detecting faults by deep learning methods is generally considered a supervised learning task, which requires numerous diverse training samples and corresponding accurate labels. Because field data with faults labeled by experienced interpreters are difficult to acquire, training samples are usually generated synthetically, in which the 1D seismic wavelets are convolved with reflectivity models. However, the 1D-convolution-based synthetic seismic images still differ from the real seismic images. Therefore, the network trained by such samples may fail to detect some faults in the field data. Full-wavefield approaches are the optimal seismic modeling methods, but they cannot be widely used at present due to the high cost. In this paper, we present an efficient approach to generate realistic synthetic seismic images by using a point-spread function (PSF)-based convolution. Because the size of a seismic sample used for deep learning training is a small range relative to the field seismic data, assuming the background velocity is close to homogeneous in such a small range, we can efficiently construct the PSF using the analytical Green’s function. With an Intel Core i9-10900K CPU, the PSF approach takes approximately 15 min to generate a seismic image of size 128 × 128 × 128, whereas it takes approximately 20 h to generate a seismic image of the same size using reverse time migration. The examples of one synthetic image and two field seismic images demonstrate that the network trained with the PSF convolution samples can predict more accurate and continuous faults than that trained with the 1D convolution samples.
Revisiting the significance of keratin expression in complex epithelia
Authors: Cohen Erez , Johnson Craig , Redmond Catherine J. , Nair Raji R. , Coulombe Pierre A. 
Published: October 2022

ABSTRACT
A large group of keratin genes (n=54 in the human genome) code for intermediate filament (IF)-forming proteins and show differential regulation in epithelial cells and tissues. Keratin expression can be highly informative about the type of epithelial tissue, differentiation status of constituent cells and biological context (e.g. normal versus diseased settings). The foundational principles underlying the use of keratin expression to gain insight about epithelial cells and tissues primarily originated in pioneering studies conducted in the 1980s. The recent emergence of single cell transcriptomics provides an opportunity to revisit these principles and gain new insight into epithelial biology. Re-analysis of single-cell RNAseq data collected from human and mouse skin has confirmed long-held views regarding the quantitative importance and pairwise regulation of specific keratin genes in keratinocytes of surface epithelia. Furthermore, such analyses confirm and extend the notion that changes in keratin gene expression occur gradually as progenitor keratinocytes commit to and undergo differentiation, and challenge the prevailing assumption that specific keratin combinations reflect a mitotic versus a post-mitotic differentiating state. Our findings provide a blueprint for similar analyses in other tissues, and warrant a more nuanced approach in the use of keratin genes as biomarkers in epithelia.
The Psychosocial Value of Employment: Evidence from a Refugee Camp
Authors: Hussam Reshmaan , Kelley Erin M. , Lane Gregory , Zahra Fatima 
Published: October 2022

Employment may be important to well-being for reasons beyond its role as an income source. This paper presents a causal estimate of the psychosocial value of employment in refugee camps in Bangladesh. We involve 745 individuals in a field experiment with three arms: a control arm, a weekly cash arm, and an employment arm of equal value. Employment raises psychosocial well-being substantially more than cash alone, and 66 percent of the employed are willing to forgo cash payments to continue working temporarily for free. Despite material poverty, those in our context both experience and recognize a nonmonetary, psychosocial value to employment. (JEL C93, D91, I31, J15, J22, O15)
Relacja między filozofią a retoryką w koncepcji Giovanniego Pico della Mirandoli
Authors: Krupecka Iwona 
Published: October 2022

Artykuł stanowi próbę analizy listu Pico della Mirandoli O języku filozofów, skierowanego do Hermolausa Barbara. W pierwszej części tekstu zaprezentowano kontekst sporu, czyli znamienne dla epoki napięcie między filozofią a retoryką, oraz krytykę filozofii scholastycznej z perspektywy humanistów. Uwyraźnienie stanowiska Barbara pozwala uchwycić sedno sporu z Pikiem, mianowicie:jakiej prawdy – prawdy o czym – powinniśmy poszukiwać? Do analizy stanowiska Pika posłużyły wypreparowane z jego tekstu opozycje: wnętrze/zewnętrze, prawda/pozór, rozum/zmysły i popędy, filozofia/retoryka-sofistyka, człowiek rozumny/człowiek pospolity. Pozwala to uchwycić myśl Pika jako z założenia paradoksalną: okazuje się, że retoryka zarazem zakrywa istotę rzeczy i ją odkrywa. Jest jednocześnie przeszkodą i nieodzownym narzędziem poznania i wyrażania myśli. Dzieje się tak ze względu na nieuchronną historyczność ludzkiej myśli.