bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bilateral Osteotomy of Pedicles for En Bloc Resection of a Malignant Tumor of the Posterior Thoracic Spine
Authors: Aibara Noriyuki , Watanabe Kota , Asano Naofumi , Suzuki Satoshi , Takahashi Yohei , Nori Satoshi , Tsuji Osahiko , Nagoshi Narihito , Yagi Mitsuru , Nakamura Masaya , Matsumoto Morio 
Published: September 2022

Case:
A 45-year-old woman had a grade II chondrosarcoma (T2N0M0G2) located at the spinous processes and laminas of T3-6 with the tumor extension into the spinal canal at T3-4. To perform en bloc tumor resection, we released or disarticulated bilateral costovertebral ligaments from T3-6 and cut the bilateral pedicles at T3-5 all from posteriorly. Then, we completed en bloc resection without violating the tumor capsule.

Conclusion:
Our novel procedure, bilateral osteotomy of pedicles for en bloc resection successfully allowed for en bloc tumor resection involving the posterior elements with wide surgical margins.
TFP5, a Peptide Derived from Cdk5 Activator p35, Protects Pancreatic β-cells from Glucose Toxicity
Authors: Liu Shunyao , Cao Shilu , Luo Hongyan , Zhang Xia , Bao Li , E Jing , Li Bo , Lan Xiaomei , Zhang Guoqing , Bao Xi , Pant Harish , Zheng Yali 
Published: September 2022

Abstract
Type 2 diabetes mellitus (T2DM) is worldwide epidemic, which challenges the health of the public. The pathological hyperactivity of cyclin-dependent kinase 5 (Cdk5) contributes to the pathogenesis of T2DM. P5, a peptide derived from the Cdk5 activator p35, shows excellent performance as a Cdk5 activity inhibitor. However, its inhibitory effect and functional regulation on Cdk5 activity needs to be confirmed. In this study, we conjugated P5 with a fluorescein isothiocyanate (FITC) tag at the N-terminus and a TAT protein transduction domain, an 11-amino acid peptide, at the C-terminus to synthesize TFP5, which was used to inhibits Cdk5 activity. We then evaluated the efficiency of TFP5 in treating T2DM. We demonstrated that TFP5 effectively penetrated pancreatic β-cells, inhibited the pathological hyperactivity of Cdk5, enhanced insulin secretion, and protected penetrated pancreatic β-cells (MIN6 cells) from apoptosis in pancreatic tissues of db/db mice (type II diabetes mice). Furthermore, we found that TFP5 reduced inflammation in pancreatic islets by reducing the expression of inflammatory cytokines, including TGF-β1, TNF-α, and IL-1β. These data indicates that the TFP5 peptide is a promising candidate for T2DM treatment.
A Stochastic Analysis of a Siqr Epidemic Model With Short and Long-term Prophylaxis
Authors: Sekkak Idriss , NASRI BOUCHRA R. , RÉMILLARD BRUNO N. , KONG JUDE DZEVELA , FATINI MOHAMED EL 
Published: September 2022

Abstract
This paper aims to incorporate a high order stochastic perturbation into a SIQR epidemic model with transient prophylaxis and lasting prophylaxis. The existence and uniqueness of the global positive solution is proven and a stochastic condition in order to study the extinction of an infectious disease is established. The existence of a stationary distribution for the stochastic epidemic model is investigated as well. Numerical simulations are conducted to support our theoretical results and an example of application with COVID-19 data from Canada is used to estimate the transmission rate and basic reproduction number while constructing a model fitting the data.
Changes in Great Vedaranyam Swamp, South-east Coast of India over last four decades: study on a crucial site for Migratory Birds of Central and East Asian flyways
Authors: Sathiyaselvam Ponnusamy , Selvaraj Ramesh Kumar , Davande Sham , Sivakumar Swaminathan , Joshi Omkar 
Published: September 2022

Abstract
Great Vedaranyam Swamp (GVS) including Point Calimere Wildlife Sanctuary, a Ramsar site is one of the largest swamps and a very crucial wintering ground for migratory birds that visit India or passing through India for their wintering sites in southern parts of Asia following Central Asian Flyway (CAF) and East Asian Australasian Flyway (EAAF). Both natural and human induced local land-use pattern changes have altered the habitats in Great Vedharanyam Swamp. The present study attempted to understand the temporal changes in the land use and land cover patterns of GVS during last three decades using Geographic Information Systems (GIS). Totally, 16 major landcover classes derived within the area of GVS for five periods of time series satellite images stretched over period of 30 years from 1988, 1999, 2002, 2013 and 2017. The Change detection for major classes of landcover such as Mangroves, Mudflats, Lagoons, Vegetation, Saltpans, Prawn farms etc. was analysed and found overall 30% of the area has changed in the GVS over 30 years`. Two major lagoons namely Seruthalaikkadu and Muthupet lagoons have shrunken in its size over the period.. Over 8,276 ha of natural mudflat areas have changed, i.e. 56% of mudflats area existed in 1988 have been disturbed. The decline in mudflats may cause a huge impact on the waterbird population that winters in GVS. Restoring and maintaining the habitats of the GVS are crucial to reduce the decline in bird populations in GVS.
Role of miRNA-4804-3p in liver injury of chronic hepatitis B through inhibit chemerin/chemokine-like receptor 1 mediated NF-κB signal pathway
Authors: Xing Tongjing , Chen Qiaoling , Li Ali , Wang Qiupeng , Zhang Qian , Xian Jianchun , Wang Xuequan 
Published: September 2022

Abstract
Objective: This study aimed to investigate the role of microRNA (miRNA)-4804-3p in liver injury during chronic hepatitis B virus(HBV) infection through inhibit chemerin/chemokine-like receptor 1(CMKLR1) mediated NF-κB signal pathway.
Methods: miRNA-4804-3p, CMKLR1, extracellular signal regulated kinase (ERK), and nuclear factor kappa B (NF-κB) mRNA expression were detected by qPCR, western blot or immunohistochemistry (IHC). A mouse model of liver injury induced by concanavalin A was established and used for experiments in vivo.
Results: Pearson correlation analysis showed a significantly negative correlation between the expression level of miRNA-4804-3p and CMKLR1 in PBMCs of patients with CHB. The miRNA-4804-3p was significantly negatively correlated with the level of alanine transaminase in patients with CHB. Previous our study have showed that the cytokine secretion of human monocyte lines could be inhibited by miRNA-4804-3p. The level of CMKLR1 gene, phosphorylated ERK (p-ERK) and NF-κB protein in cells transfected with miRNA-4804-3p mimics was significantly decreased compared with that of the negative control group. The levels of IL-2, IL-10, TNF-α and IFN-γ in cell culture supernatant were no significant difference between miRNA-4804-3p mimics+CMKLR1 knocdown group and negative control group. The level of AST after injection with agomiRNA-4804-3p significantly decreased compared with that of pre-injection. The amount of inflammation in liver tissue of mice injected with agomiRNA-4804-3p was significantly lower compared with that of the negative control group, but the amount of fibrosis had no significant alteration.
Conclusions: miRNA-4804-3p may inhibit chemerin/CMKLR1–mediated NF-κB signal pathway and be involved in regulating liver inflammatory injury in CHB. This study might not only provide a new explanation for the pathogenesis of CHB, but also provide new potential molecular markers for the prevention and treatment of CHB.
Tumor microenvironment landscape of epithelial ovarian carcinoma highlights EGR1-driven mechanisms of tumor invasion at single-cell resolution
Authors: Guo Qiuyan , Zhang Yuanfu , Sun Shu , Qi Yue , Dai Yifan , Hao Yangyang , Xin Mengyu , Xu Rongji , Chen Hongyan , Wu Xiaoting , Liu Qian , Kong Congcong , Zhang Guangmei , Wang Peng 
Published: September 2022

Abstract
Identifying underlying molecular mechanisms and biomarkers of epithelial ovarian carcinoma (EOC) proliferation and metastasis remains challenging. Patients of EOC are usually diagnosed at an advanced stage and the availability of invasion-related targets is limited. Herein, we explored the single-cell RNA sequencing (scRNA-seq) dataset of EOC and defined tumor physiological reprograming compared to bulk RNA-seq. The energy metabolism and anti-apoptotic pathway was found as critical contributors to intratumor heterogeneity. Moreover, hypoxia, oxidative phosphorylation (OXPHOS) and glycolysis were positively correlated, which have biologically activity trajectories during epithelial mesenchymal transition (EMT). The HMGH1, EGR1 and RUNX1 were found to be critical inducers of the EMT process in EOC. Experimental validation revealed that suppressed EGR1 decreased the expression of FAS and HSPG2 and associating with EMT progression in EOC. In tumor microenvironment (TME), CAFs were found have significant contribution to tumor immune infiltration and metastasis and accumulation of CAFs was associated with poorer patient survival. In conclusion, physiological features and molecular mechanisms in the TME of EOC were revealed and provided effective targets for the suppression of tumor metastasis.
Prognostic value of morning blood pressure surge in chronic kidney disease
Authors: Ma Zhilan , Wang Yan , Guo Xiaoyan , Ma Lan , Liu Yonghua , Zhou Xiaoling 
Published: September 2022

Abstract
Objective: To investigate the prognostic value of morning blood pressure surge (MBPS) in chronic kidney disease (CKD).
Methods: We investigated the occurrence of a composite endpoints (coronary events, stroke, and heart failure requiring hospitalization, end-stage renal disease, death) in 153 patients (aged ³18 years) with a diagnosis of in CKD. Based on MBPS defined as ≥35 mmHg, patients were divided into a two groups: with MBPS (n = 50) and without MBPS (n = 103). The follow-up period was ³10 months. Baseline demographic, laboratory and follow-up data were collected. The clinical characteristics of the two groups were compared. The relationships between MBPS and endpoint events were analyzed using the Kaplan–Meier method and Cox regression model.
Results: In total, 153 patients ( mean age 41.8 years; 56.86% males) were included in this study. During the follow-up period (mean 4.3 years), 34 endpoint events occurred. After adjustment for the covariates, the risk of Cardiovascular and cerebrovascular events, end-stage renal disease and death remained significantly higher in patients with MBPS [hazard ratio (HR) and 95% confidence interval (CI) 3.124 (1.096–9.130)]. Among the other variables, 24-h systolic blood pressure, and night-time and daytime pulse pressures remained significantly associated with outcome in patients of CKD [1.789 (1.205–2.654), 1.710 (1.200–2.437), and 1.318 (1.096–1.586), respectively].
Conclusion: MBPS was identified as an independent prognostic factor for composite endpoint events (coronary events, stroke, and heart failure requiring hospitalization, end-stage kidney disease) patients with chronic kidney disease patients.
Combined Endoscopic and Microscopic Surgery for Complex Skull Base Tumors: A Single-Center Case Series Study in China
Authors: Ding Yu , Lu XiaoCheng , Pan Pengjie , Sun Liang , Zhu Shixin , Yu ZhengQuan , Wu Jiang , Chen Gang 
Published: September 2022

Abstract
Objective
Surgical treatment of skull base tumors is considered very difficult, especially when the lesion located at the cavernous sinus, petroclival region, and the foramen magnum. The evolution of endoscopic techniques provides effective therapeutic strategies for skull base tumors. In this study, the authors describe a combined microscopic and endoscopic technique to minimize blind spots during microsurgery and investigate its value in resecting complex skull base tumors.
Methods
A retrospective analysis was performed on 51 patients who underwent the combined microscopic-endoscopic surgery to remove skull base tumors from January 2017 to February 2021. The extent of resection and neurological outcome were analyzed to evaluate the feasibility and applicability of the combined microscopic-endoscopic technique in skull base surgery.
Results
Gross total resection was achieved in 45 patients (82%) and near-total resection in 6 (11.8%). Total resection was determined under the microscope in 19 patients, but the residual tumor was detected after endoscopic exploration. New postoperative cranial nerve deficits were reported in 9 patients (17.6%). Of the patients with new-onset cranial nerve deficits, four patients (44.4%) had partial recovery, one (11.1%) patient had no change, and four (44.4%) completely recovered.
Conclusions
The combined microscopic-endoscopic technique effectively integrates microscopes and endoscopes to avoid damaging the surrounding neurovascular structures during surgery. This technique allows for a minimally invasive maximal resection, resulting in a better prognosis for skull base tumors.
Synergistic gel formation in geopolymers of superior mechanical strength synthesized with volcanic ash and slag
Authors: Yanjin Zheng , Rao Feng , Tian Xiang , Lin Shenjian 
Published: September 2022

Abstract
The present work studies gel evolution and microstructure of geopolymers synthesized with volcanic ash (VA) and blast furnace slag (BFS). The synthesis parameters such as BFS proportions on geopolymers formation were investigated. Gel evolution and microstructure of the geopolymers were studied by FTIR, X-ray diffraction (XRD), 29Si NMR spectroscopy, and scanning electron microscopy measurements. Silicate gels (N−S−H) were mainly formed in VA based geopolymers of low compressive strength (14.07 MPa). While with VA and BFS each account for 50%, VA-BFS based geopolymers possessed a compressive strength of 55.6 MPa, as well as the homogeneous C−(A)−S−H and N−A−S−H gels were formed. The C−(A)−S−H and N−A−S−H gels show synergistic effects on mechanical property of the geopolymers. This work provides a clue for the synthesis of geopolymers with superior mechanical properties in areas of architecture.
An Investigation of Prescription Indicators in Outpatient Services among Social Security Organization Facilities in Iran
Authors: Aeenparast Afsoon , Mehrizi Ali Asghar Haeri , Farzadi Faranak 
Published: September 2022

Abstract
Background: Drug is a very important component of health care system and rational use of it is very influential factor in health. The aim of this study was to estimate drug prescription indicators in outpatient services in Social Security Organization’s (SSO) healthcare facilities and also compare them with World Health Organization (WHO) standards.
Methods: This was a cross-sectional study. The data of all prescribed drugs for outpatient visits in 2017 - 2018 were extracted from SSO database. The data was categorized in 4 main sub groups: patient characters, provider characters, service characters and healthcare facility characters.. After data acquisition, data cleaning phase was done and irrelevant data were detected and deleted before final analysis. SPSS and IBM modeler soft wares were used for data analysis. Logistic regression models also was used for detecting inappropriate drug prescription risk factors.
Results: Rapid analysis of extracted data indicated that in 2017 about 150, 981,752 drugs were prescribed for all of outpatients in SSO healthcare facilities in Iran. The aggregation of data indicated that these amount of medicines were prescribed in 45,312,540 physicians’ prescriptions.
The average number of drug items per outpatient prescriptions was estimated 3.33. Rate of prescriptions with injection was 17.5 percent and rate of prescriptions with antibiotic was 37.5 percent.
Logistic Regression Analysis indicated that, patient sex and age, providers’ specialty, type of facility and time of outpatient visit were some indicators that was associated with the risk of inappropriate prescription. (p<0.001).
Conclusions:Rational drug prescription has an important impact on population health and the drug industry economy. In this study all drug prescription criteria were higher than WHO recommended limits. It seems that these pattern will increase the risk of drug interactions and also the affect the cost-effective of drugs. These type of researches provide good evidences for analyzing the present situation and strategies for improving it. It is estimated that providing the monthly and annual report to providers alongside considering some prescription policies for physicians will be helpful in improving the current prescription pattern through the country specially in SSO.
Ethics code: IR.ACECR.IBCRC.REC.1396.2
Study on ambient air quality in Shenyang city during the 13th Five-Year Plan period
Authors: Huang Yu , Chen Xi , Li Hui , Fan Guangjian , Xu Xinyang 
Published: September 2022

Abstract
During the 13th Five-Year Plan period (2016–2020), China has comprehensively strengthened the prevention and control of air pollution. The comprehensive treatment of air pollution has been fully implemented since 2016 and the ambient air quality in China has been improved. In order to study on changes and influencing factors of ambient air quality in the central economic zone of inland provinces in the Northeast China during the 13th Five-Year Plan period, ambient air quality in Heping District of Shenyang from 2016 to 2020 was monitored. The ambient air quality was monitored for seven air pollutants PM10, PM2.5, SO2, NO2, CO, O3 and dust fall at five different locations. The study presents the ambient air quality has significantly improved over the past five years. And the results of grey relational analysis show that secondary industry and green area are the main affecting factors of the ambient air quality in addition to population factors. Appropriately reducing the proportion of secondary industry and increasing the green area are effective measures to improve ambient air quality in the future. These findings would provide a basis for city management to improve the ambient air quality in the Northeast China in the future.
Resting State Functional Connectivity as a Marker of Internalizing Disorder Onset in High-Risk Youth
Authors: Pawlak McKinley , Bray Signe , Kopala-Sibley Daniel C. 
Published: September 2022

Abstract
While research has linked alterations in functional connectivity of resting state brain networks including the default mode (DMN), cognitive control (CCN), and salience networks (SN) to depression and anxiety, little research has examined whether these alterations may be premorbid vulnerabilities. This study examined resting state functional connectivity (RSFC) of the CCN, DMN, and SN as neural markers of risk for developing a first lifetime onset of a depressive or anxiety disorder in adolescents at high familial risk for these disorders. At baseline, 135 participants aged 11–17 completed resting-state functional magnetic resonance imaging (rs-fMRI), measures of internalizing symptoms, and diagnostic interviews to assess history of depressive and anxiety disorders. Diagnostic assessments were completed again at 9- or 18-month follow-up for 112 participants. At baseline, increased CCN connectivity to areas of the visual network, and decreased connectivity between the SN and the precentral gyrus, predicted an increased likelihood of an internalizing disorder onset at follow-up. Altered connectivity between these regions may represent a pre-morbid risk factor for developing a clinically significant onset of an internalizing disorder. Results may have implications for understanding the neural bases of internalizing disorder episodes and for early identification and prevention efforts.
Multiple waves of COVID-19: A pathway model approach
Authors: Vasconcelos Giovani , Pessoa Nathan L. , Silva Natan B. , Macêdo Antônio M. S. , Brum Arthur A. , Ospina Raydonal , Tirnakli Ugur 
Published: September 2022

Abstract
A generalized pathway model, with time dependent parameters, is applied to describe the mortality curves of the COVID-19 disease for several countries that exhibit multiple waves of infections. The pathway approach adopted here is formulated explicitly in time, in the sense that the model’s growth rate for the number of deaths or infections is written as an explicit function of time, rather than in terms of the cumulative quantity itself. This allows for a direct fit of the model to daily data (new deaths or new cases) without the need of any integration. The model is applied to COVID-19 mortality curves for ten selected countries and found to be in very good agreement with the data for all cases considered. From the fitted theoretical curves for a given location, relevant epidemiological information can be extracted, such as the starting and peak dates for each successive wave. It is argued that obtaining reliable estimates for such characteristic points is important for studying the effectiveness of interventions and the possible negative impact of their relaxation, as it allows for a direct comparison of the time of adoption/relaxation of control measures with the peaks and troughs of the epidemic curve.
Simplified procedure for rapidly estimating inelastic responses of numerous high-rise buildings with reinforced concrete shear walls
Authors: Suwansaya Phichaya , Warnitchai Pennung 
Published: September 2022

Abstract
Nonlinear response history analysis (NLRHA) is considered the most accurate procedure for evaluating the seismic performance of high-rise buildings. However, it requires considerable expertise and a significant amount of analysis time, making it inappropriate for evaluating numerous high-rise buildings; for example, estimating the seismic losses of a city for an earthquake scenario. To overcome this limitation, a simplified procedure developed based on the uncoupled modal response history analysis (UMRHA) and coupled shear-flexural cantilever beam model (CSFCBM) is proposed. The underlying assumption is that the UMRHA procedure can compute the nonlinear seismic responses mode-by-mode, where each vibration mode is assumed to behave as a single-degree-of-freedom system. The nonlinear seismic responses are approximately represented by the sum of the modal responses of a few vibration modes. However, UMRHA requires knowledge of the modal properties and modal hysteretic behaviors. Therefore, the CSFCBM is introduced here to estimate the required modal properties and modal hysteretic behaviors. The inelastic seismic demands of the building can be determined using the UMRHA procedure with the computed modal properties obtained by CSFCBM. The accuracy of this proposed procedure is verified considering four high-rise buildings of 19, 30, 34, and 45 stories with reinforced concrete shear walls. The inelastic demands computed by the NLRHA procedure are used as a benchmark and compared with those of the proposed procedure. The results indicate that the proposed procedure provides reasonably accurate demand estimations for all case study buildings with significantly less analysis time than that required by NLRHA.