bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical significance of tumor-infiltrating lymphocytes investigated using routine H&E slides in small cell lung cancer
Authors: Zhou Guangrun , Zheng Jifang , Chen Zhiwei , Hu Dan , Li Suyu , Zhuang Wu , He Zhiyong , Lin Gen , Wu Biao , Zhang Wei , Fang Weimin , Zheng Fei , Wang Jiezhong , Chen Gang , Chen Mingqiu 
Published: July 2022

Abstract
Background
Tumor-infiltrating lymphocytes (TILs), investigated using routine hematoxylin and eosin (H&E)-stained section slides (H&E-sTILs), provide a robust prognostic biomarker in various types of solid cancer. The purpose of the present study was to investigate the prognostic significance of H&E-sTILs in patients with small cell lung cancer (SCLC).

Methods
The clinical data of patients with SCLC who had been treated in our cancer center between January 2013 and October 2019 were collected and retrospectively reviewed. The H&E-sTILs were re-assessed by two experienced pathologists independently. H&E-sTILs that affected the overall survival (OS), progression free survival (PFS) and brain-metastasis free survival (BMFS) rates were explored using the Kaplan–Meier method, and the log-rank test was used to assess the differences. Multivariate analysis was subsequently performed using the Cox proportion hazards model.

Results
A total of 159 patients with SCLC who fulfilled the inclusion criteria were enrolled in the current study. The OS rates at 1, 2 and 3 years were 59.8, 28.6 and 19.8%, respectively, for the whole group. The 3-year OS, PFS and BMFS rates for the H&E-sTILs(+) and H&E-sTILs(−) groups were 25.1% cf. 5.1% (P = 0.030), 14.0% cf. 4.0% (P = 0.013), and 66.0% cf. 11.4% (P = 0.023), respectively. Multivariate analyses subsequently revealed that H&E-sTILs, clinical M stage, the cycles of chemotherapy and short-term response to thoracic radiotherapy were independent factors affecting OS, whereas H&E-sTILs, clinical N stage, clinical M stage and short-term response to chemotherapy were factors affecting PFS. The H&E-sTILs affected OS, PFS and BMFS simultaneously.

Conclusions
The results of this retrospective study have shown that H&E-sTILs may be considered as a prognostic biomarker affecting the short-term response to treatment, and they are the one and only risk factor for BMFS. However, due to the limitations of the nature of the retrospective design and shortcomings in visually assessing the TILs based on the H&E-stained slides, further prospective studies are required to confirm these conclusions.
Donepezil ameliorates Aβ pathology but not tau pathology in 5xFAD mice
Authors: Choi Hee-Jeong , Park Jin-Hee , Jeong Yoo Joo , Hwang Jeong-Woo , Lee Soojung , Lee Heeyong , Seol Eunyoung , Kim Ik-whi , Cha Byung-Yoon , Seo Jinsoo , Moon Minho , Hoe Hyang-Sook 
Published: July 2022

AbstractThe cholinesterase inhibitor donepezil is used to improve Aβ pathology and cognitive function in patients with Alzheimer’s disease (AD). However, the impact of donepezil on tau pathology is unclear. Thus, we examined the effects of donepezil on Aβ and tau pathology in 5xFAD mice (a model of AD) in this study. We found that intraperitoneal injection of donepezil (1 mg/kg, i.p.) exhibited significant reductions in Aβ plaque number in the cortex and hippocampal DG region. In addition, donepezil treatment (1 mg/kg, i.p.) reduced Aβ-mediated microglial and, to a lesser extent, astrocytic activation in 5xFAD mice. However, neither intraperitoneal/oral injection of donepezil nor oral injection of rivastigmine altered tau phosphorylation at Thr212/Ser214 (AT100), Thr396, and Thr231 in 5xFAD mice. Surprisingly, we observed that intraperitoneal/oral injection of donepezil treatment significantly increased tau phosphorylation at Thr212 in 5xFAD mice. Taken together, these data suggest that intraperitoneal injection of donepezil suppresses Aβ pathology but not tau pathology in 5xFAD mice.
The effect of gestational age, low birth weight and parity on birth asphyxia among neonates in sub-Saharan Africa: systematic review and meta-analysis: 2021
Authors: Techane Masresha Asmare , Alemu Tewodros Getaneh , Wubneh Chalachew Adugna , Belay Getaneh Mulualem , Tamir Tadesse Tarik , Muhye Addis Bilal , Kassie Destaye Guadie , Wondim Amare , Terefe Bewuketu , Tarekegn Bethelihem Tigabu , Ali Mohammed Seid , Fentie Beletech , Gonete Almaz Tefera , Tekeba Berhan , Kassa Selam Fisiha , Desta Bogale Kassahun , Ayele Amare Demsie , Dessie Melkamu Tilahun , Atalell Kendalem Asmare , Assimamaw Nega Tezera 
Published: July 2022

Abstract
Background
Despite simple and proven cost-effective measures were available to prevent birth asphyxia; studies suggested that there has been limited progress in preventing birth asphyxia even in healthy full-term neonates. In Sub-Saharan Africa, Inconsistency of magnitude of birth asphyxia and its association gestational age, Low birth Weight and Parity among different studies has been observed through time.

Objective
This study aimed to estimate the Pooled magnitude of birth asphyxia and its association with gestational age, Low birth Weight and Parity among Neonates in Sub-Saharan Africa.

Method
PubMed, Cochrane library and Google scholar databases were searched for relevant literatures. In addition, reference lists of included studies were retrieved to obtain birth asphyxia related articles. Appropriate search term was established and used to retrieve studies from databases. Searching was limited to cohort, cross-sectional, and case-control studies conducted in Sub-Saharan africa and published in English language. Joanna Briggs Institute Meta-Analysis of Statistics Assessment and Review Instrument (JBI-MAStARI) was used for critical appraisal of studies. Heterogeneity across the included studies was evaluated by using the inconsistency index (I2) test. Funnel plot and the Egger’s regression test were used to test publication bias. A weighted inverse variance random effects- model was used to estimate the pooled prevalence of birth asphyxia among neonates in Sub-Saharan Africa. STATA™ version 11softwarewasused to conduct the meta-analysis.

Result
A total of 40 studies with 176,334 study participants were included in this systematic review and meta-analysis. The overall pooled magnitude of birth asphyxia in Sub-Saharan Africa was 17.28% (95% CI; (15.5, 19.04). low birth weight (AOR = 2.58(95% CI: 1.36, 4.88)), primigravida (AOR = 1.15 (95% CI: 0.84, 1.46) andMeconium-stained amniotic fluid (AOR = 6(95% CI: 3.69, 9.74)) werevariables significantly associated with the pooled prevalence of birth asphyxia.

Conclusion
The pooled magnitude of birth asphyxia was found to be high in Sub-Saharan Africa. Low birthweight and Meconium-stained amniotic fluid were variables significantly associated with birth asphyxia in Sub-Saharan Africa. Hence, it is better to develop early detection and management strategies for the affected neonates with low birth weight and born from mothers intrapartum meconium stained amniotic fluid.
Systematic review of Plasmodium knowlesi in Indonesia: a risk of emergence in the context of capital relocation to Borneo?
Authors: Bin Said Ibrahim , Kouakou Yobouet Ines , Omorou Roukayatou , Bienvenu Anne-Lise , Ahmed Kamruddin , Culleton Richard , Picot Stephane 
Published: July 2022

Abstract
Background
The Indonesian Republic plans to relocate its capital from Jakarta to East Kalimantan, Borneo Island, in the next few years. This relocation may be associated with deforestation, decreased biodiversity, and an increased risk of emerging zoonotic infections, including Plasmodium knowlesi malaria. The Malaysian part of Borneo Island is one of the main hotspots of P. knowlesi malaria.

Methods
Considering this risk, we evaluated the transmission dynamics of P. knowlesi in the Indonesian Archipelago based on a literature search and extensive review of data from the Indonesian Ministry of Health.

Results
We report that 545 P. knowlesi cases were documented in Indonesia, mainly in the Aceh and North Sumatra provinces, with 95% of these occurring in the last 4 years.

Conclusions
The main P. knowlesi vectors are present in the area of the future capital, requiring strengthened surveillance to reduce the risk of emerging cases in a rapidly growing population.
Maternal SMCHD1 controls both imprinted Xist expression and imprinted X chromosome inactivation
Authors: Wanigasuriya Iromi , Kinkel Sarah A. , Beck Tamara , Roper Ellise A. , Breslin Kelsey , Lee Heather J. , Keniry Andrew , Ritchie Matthew E. , Blewitt Marnie E. , Gouil Quentin 
Published: July 2022

AbstractEmbryonic development is dependent on the maternal supply of proteins through the oocyte, including factors setting up the adequate epigenetic patterning of the zygotic genome. We previously reported that one such factor is the epigenetic repressor SMCHD1, whose maternal supply controls autosomal imprinted expression in mouse preimplantation embryos and mid-gestation placenta. In mouse preimplantation embryos, X chromosome inactivation is also an imprinted process. Combining genomics and imaging, we show that maternal SMCHD1 is required not only for the imprinted expression ofXistin preimplantation embryos, but also for the efficient silencing of the inactive X in both the preimplantation embryo and mid-gestation placenta. These results expand the role of SMCHD1 in enforcing the silencing of Polycomb targets. The inability of zygotic SMCHD1 to fully restore imprinted X inactivation further points to maternal SMCHD1’s role in setting up the appropriate chromatin environment during preimplantation development, a critical window of epigenetic remodelling.
Systemic lupus erythematosus with trisomy X: a case report and review of the literature
Authors: Luo Fang , Ye Qiao , Shen Jie 
Published: July 2022

AbstractBackgroundThe cause of systemic lupus erythematosus is not completely clear so far, but the prevalence of systemic lupus erythematosus is significantly increased in people with additional X chromosomes.Case presentationWe report a 17-year-old Chinese female patient with systemic lupus erythematosus complicated with trisomy X, accompanied by lupus nephritis, pancytopenia, hemolytic anemia, and multiserous effusion. The patient recovered well after treatment and returned regularly. We review the previously reported cases to summarize the clinical characteristics of these patients.ConclusionThe additional X chromosome is related to the development of systemic lupus erythematosus. Whether it is a subtype of systemic lupus erythematosus remains to be further confirmed.
Extracellular matrix derived from Wharton’s Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGF
Authors: Ma Beilei , Wang Tengkai , Li Juan , Wang Qian 
Published: July 2022

AbstractBackgroundAngiogenesis is required in many physiological conditions, including bone regeneration, wound healing, and tissue regeneration. Mesenchymal stem cells-derived extracellular matrix (MSCs-ECM) could guide intricate cellular and tissue processes such as homeostasis, healing and regeneration.MethodsThe purpose of this study is to explore the effect and mechanism of ECM derived from decellularized Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) on endothelial cell viability and angiogenesis. The human umbilical vein endothelial cells (HUVECs) were pretreated with WJ-MSCs ECM for 2d/7d/14d, respectively. After pretreatment, the angiogenesis ability of HUVECs was detected.ResultsIn this study, we found for the first time that WJ-MSCs ECM could improve the angiogenesis ability of HUVECs with a time-dependent manner in vitro. Mechanically, WJ-MSCs ECM activated the focal adhesion kinase (FAK)/P38 signaling pathway via integrin αVβ3, which further promoted the expression of the cellular (c)-Myc. Further, c-Myc increased histone acetylation levels of the vascular endothelial growth factor (VEGF) promoter by recruiting P300, which ultimately promoting VEGF expression.ConclusionsECM derived from Wharton’s Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGF.This study is expected to provide a new approach to promote angiogenesis in bone and tissue regeneration.
Oxidative stress-induced endothelial cells-derived exosomes accelerate skin flap survival through Lnc NEAT1-mediated promotion of endothelial progenitor cell function
Authors: Guo Linlin , Chen Yuxuan , Feng Xiaoling , Sun Di , Sun Jiaming , Mou Shan , Zhao Kangcheng , An Ran 
Published: July 2022

AbstractBackgroundFlap transplantation is commonly used in reconstructive surgery. A prerequisite for skin flap survival is sufficient blood supply. However, such approaches remain unclear. This study aimed to explore the underlying mechanisms of exosomes derived from human umbilical vascular endothelial cells (HUVECs) exposed to oxidative stress on endothelial progenitor cells (EPCs) and their subsequent influence on the survival of skin flaps.MethodsHUVECs were treated with various concentrations of H2O2to establish an oxidative stress model. To investigate the effects of H2O2-HUVEC-Exos and HUVEC-Exos, Cell Counting Kit-8, tube formation, invasion assays, and quantitative real-time polymerase chain reaction (qRT-PCR) were performed in EPCs. Microarray analysis was used to reveal the differentially expressed long non-coding RNAs (lncRNAs) in the H2O2-HUVEC-Exos and HUVEC-Exos. In addition, gene silencing and western blotting were employed to determine the mechanism behind lncRNA nuclear enrichment enriched transcript 1 (Lnc NEAT1) in EPCs. Further, a rat skin flap model was used to determine the role of the exosomes in skin flap survival in vivo.ResultsHUVECs were stimulated with 100 μmol/L H2O2for 12 h to establish an oxidative stress model. H2O2-HUVEC-Exos promoted the proliferation, tube formation, and invasion of EPCs and remarkably increased skin flap survival compared to the HUVEC-Exos and control groups. Sequencing of exosome RNAs revealed that the Lnc NEAT1 level was dramatically increased in the H2O2-HUVEC-Exos, leading to activation of the Wnt/β-catenin signaling pathway. Comparatively, knockdown of Lnc NEAT1 in HUVEC-Exos and H2O2-HUVEC-Exos significantly inhibits the angiogenic capacity of EPCs, reduced the survival area of skin flap and downregulated the expression levels of Wnt/β-catenin signaling pathway proteins, whereas Wnt agonist partly reversed the negative effect of NEAT1 downregulation on EPCs through the Wnt/β-catenin signaling pathway.ConclusionsExosomes derived from HUVECs stimulated by oxidative stress significantly promoted the pro-angiogenic ability of EPCs through the Wnt/β-catenin signaling pathway mediated by Lnc NEAT1 and hence enhanced random flap survival in vivo. Therefore, the application of H2O2-HUVEC-Exos may serve as an alternative therapy for improving random skin flap survival.
Integrating criticality concepts into road network disruption assessments for volcanic eruptions
Authors: Hayes Josh L. , Biass Sébastien , Jenkins Susanna F. , Meredith Elinor S. , Williams George T. 
Published: July 2022

AbstractRoad networks in volcanically active regions can be exposed to various volcanic hazards from multiple volcanoes. Exposure assessments are often used in these environments to prioritise risk management and mitigation efforts towards volcanoes or hazards that present the greatest threat. Typically, road exposure has been assessed by quantifying the amount of road network affected by different hazards and/or hazard intensity. Whilst this approach is computationally efficient, it largely fails to consider the relative importance of road segments within the network (i.e., road criticality). However, road criticality is an important indicator of the disruption that may be caused by an eruption. In this work, we aim to integrate road criticality concepts to enhance typical volcanic eruption road exposure assessments into road disruption assessments. We use three key components to quantify disruption: a) road criticality, b) impact severity, and c) affected road quantity. Two case study eruptions: Merapi 2010 and Kelud 2014, both in Java, Indonesia, are used to demonstrate the usefulness of integrating road criticality into road disruption assessments from volcanic eruptions. We found that disruption of the road network from the Kelud 2014 case study was an order of magnitude greater than the Merapi 2010 case study. This is primarily driven by the more widely dispersed tephra fall from the Kelud 2014 event, which affected nearly 28% of Java’s road network length, compared to Merapi 2010, which affected 1.5%. We also identified potential disruption hotspots that were affected by both of these case study eruptions. At Merapi, roads that carry traffic directly away from the summit, those that cross major valleys, and the major Yogyakarta-Magelang highway were key disruption hotspots, which has implications for moving large volumes of traffic efficiently, such as in an evacuation. The Kelud case study highlighted the potential impacts of widespread tephra falls on socio-economic activity and connectivity of large urban centres. Our approach has been designed such that it can be applied entirely using open-sourced datasets. Therefore, the approach to integrating road criticality in this paper can be used, applied, and adapted to assess road network disruption at any volcano in the world.
PICF-LDA: a topic enhanced LDA with probability incremental correction factor for Web API service clustering
Authors: Shen Jiaji , Huang Wen , Hu Qiang 
Published: July 2022

AbstractWeb API is a popular way to organize network services in cloud computing environment. However, it is a challenge to find an appropriate service for the requestor from massive Web API services. Service clustering can improve the efficiency of service discovery for its ability of reducing search space. Latent Dirichlet Allocation (LDA) is the most frequently used topic model in service clustering. To further improve the topic representation ability of LDA, we propose a new variant model of LDA with probability incremental correction factor (PICF-LDA) to generate the high-quality service representation vectors (SRVs) for Web API services. We first compute the words’ topic contribution degree (TCD) in the service description text by its context weight and part-of-speech (POS) weight. Then the probability incremental correction factor (PICF) for a word is designed based on TCD and the word’s maximum topic probability value. PICF is used to correct the probability distributions in SRVs. Experiments show that PICF-LDA has a better performance than LDA, the variant LDA models and other state-of-the-art topic models in service clustering.
Outcomes of laparoscopic versus open total gastrectomy with D2 lymphadenectomy for gastric cancer: a systematic review and meta-analysis
Authors: Yang Yongpu , Chen Yuyan , Hu Yilin , Feng Ying , Mao Qinsheng , Xue Wanjiang 
Published: July 2022

AbstractBackgroundThe effectiveness of laparoscopic total gastrectomy with D2 lymphadenectomy (LTGD2) remains controversial. This meta-analysis compares surgical and survival outcomes of LTGD2 and open total gastrectomy with D2 lymphadenectomy (OTGD2) for gastric cancer.MethodsControlled studies comparing LTGD2 and OTGD2 published before November 2021 were retrieved via database searches. We compared intraoperative outcomes, pathological data, postoperative outcomes, 5-year disease-free survival (DFS), and overall survival (OS).Results17 studies were included, containing 4742 patients. Compared with OTGD2, the LTGD2 group had less blood loss (mean difference [MD] = − 122.48; 95% CI: − 187.60, − 57.37; P = 0.0002), fewer analgesic medication (MD = -2.48; 95% CI: − 2.69, − 2.27; P < 0.00001), earlier first flatus (MD = − 1.03; 95% CI: − 1.80, − 0.26; P = 0.009), earlier initial food intake (MD = − 0.89; 95% CI: − 1.09, − 0.68; P < 0.00001) and shorter hospital stay (MD = − 3.24; 95% CI: − 3.75, − 2.73; P < 0.00001). The LTGD2 group had lower postoperative total complication ratio (OR = 0.76; 95% CI: 0.62, 0.92; P = 0.006), incision (OR = 0.50; 95% CI:0.31, 0.79; P = 0.003) and pulmonary (OR = 0.57; 95% CI: 0.34, 0.96; P = 0.03) complication rates, but similar rates of other complications and mortality. Total number of dissected lymph nodes were similar, but the number of No. 10 dissected nodes was less with LTGD2 (MD = − 0.31; 95% CI: − 0.46, − 0.16; P < 0.0001). There was no difference in 5-year OS (P = 0.19) and DFS (P = 0.34) between LTGD2 and OTGD2 groups.ConclusionsLTGD2 produces small trauma, fast postoperative recovery and small length of hospital stays than OTGD2, and had similar long-term clinical efficacy as OTGD2. However, these results still need further high-quality prospective randomized controlled trials confirmation.
Ketogenic diets slow melanoma growth in vivo regardless of tumor genetics and metabolic plasticity
Authors: Weber Daniela D. , Aminzadeh-Gohari Sepideh , Thapa Maheshwor , Redtenbacher Anna-Sophia , Catalano Luca , Capelôa Tânia , Vazeille Thibaut , Emberger Michael , Felder Thomas K. , Feichtinger René G. , Koelblinger Peter , Dallmann Guido , Sonveaux Pierre , Lang Roland , Kofler Barbara 
Published: July 2022

Abstract
Background
Growing evidence supports the use of low-carbohydrate/high-fat ketogenic diets as an adjunctive cancer therapy. However, it is unclear which genetic, metabolic, or immunological factors contribute to the beneficial effect of ketogenic diets. Therefore, we investigated the effect of ketogenic diets on the progression and metabolism of genetically and metabolically heterogeneous melanoma xenografts, as well as on the development of melanoma metastases in mice with a functional immune system.

Methods
Mice bearing BRAF mutant, NRAS mutant, and wild-type melanoma xenografts as well as mice bearing highly metastatic melanoma allografts were fed with a control diet or ketogenic diets, differing in their triglyceride composition, to evaluate the effect of ketogenic diets on tumor growth and metastasis. We performed an in-depth targeted metabolomics analysis in plasma and xenografts to elucidate potential antitumor mechanisms in vivo.

Results
We show that ketogenic diets effectively reduced tumor growth in immunocompromised mice bearing genetically and metabolically heterogeneous human melanoma xenografts. Furthermore, the ketogenic diets exerted a metastasis-reducing effect in the immunocompetent syngeneic melanoma mouse model. Targeted analysis of plasma and tumor metabolomes revealed that ketogenic diets induced distinct changes in amino acid metabolism. Interestingly, ketogenic diets reduced the levels of alpha-amino adipic acid, a biomarker of cancer, in circulation to levels observed in tumor-free mice. Additionally, alpha-amino adipic acid was reduced in xenografts by ketogenic diets. Moreover, the ketogenic diets increased sphingomyelin levels in plasma and the hydroxylation of sphingomyelins and acylcarnitines in tumors.

Conclusions
Ketogenic diets induced antitumor effects toward melanoma regardless of the tumors´ genetic background, its metabolic signature, and the host immune status. Moreover, ketogenic diets simultaneously affected multiple metabolic pathways to create an unfavorable environment for melanoma cell proliferation, supporting their potential as a complementary nutritional approach to melanoma therapy.