bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Work Requirements on Mental Health of Subsidized Housing Recipients: Evidence from the Housing Authority of Champaign County, Illinois
Authors: Lee Han Bum , McNamara Paul E. 
Published: March 2022

AbstractThis study examines the early effect of work requirements on mental health of subsidized housing recipients. The Housing Authority of Champaign County (HACC) enforced work requirements for working-aged and non-disabled recipients and supported their transition to work with case management services. In addition, non-compliance with work requirements might result in the loss of housing subsidy. We collected survey data 1 year prior to and 1 year following the implementation of HACC’s work requirements to compare changes in mental health measures between work-able recipients who were and were not subject to work requirements. We calculate the average treatment effects on the treated estimates obtained from difference-in-differences with propensity score matching to control potential bias stemming from unobserved time-invariant variables and support the model’s parallel trend assumption. Our main results show that, during the early phase of work requirements, impacted heads of households were predicted to increase depression scores and decrease hopefulness scores relative to changes in mental health measures of the matched control groups over the same period. Recipients’ mental health would play a critical role in the success of HACC’s self-sufficiency strategies, and therefore the housing authority may strengthen the transition of hard-to-employ recipients into the workforce by offering careful assessment and screening to identify recipients with serious mental health problems or other employment barriers and reinforcing their access to supportive health and social services.
LDHA Desuccinylase Sirtuin 5 as a Novel Cancer Metastatic Stimulator in Aggressive Prostate Cancer
Authors: Kwon Oh Kwang , Bang In Hyuk , Choi So Young , Jeon Ju Mi , Na Ann-Yae , Gao Yan , Cho Sam Seok , Ki Sung Hwan , Choe Youngshik , Lee Jun Nyung , Ha Yun-Sok , Bae Eun Ju , Kwon Tae Gyun , Park Byung-Hyun , Lee Sangkyu 
Published: March 2022

Abstract
Prostate cancer (PCa) is the most commonly diagnosed genital cancer in men worldwide. Around 80% of the patients who developed advanced PCa suffered from bone metastasis, with a sharp drop in the survival rate. Despite great efforts, the detailed mechanisms underlying castration-resistant PCa (CRPC) remain unclear. Sirtuin 5 (SIRT5), an NAD+-dependent desuccinylase, is hypothesized to be a key regulator of various cancers. However, compared to other SIRTs, the role of SIRT5 in cancer has not been extensively studied. Here, we revealed significantly decreased SIRT5 levels in aggressive PCa cells relative to the PCa stages. The correlation between the decrease in the SIRT5 level and the patient’s reduced survival rate was also confirmed. Using quantitative global succinylome analysis, we characterized a significant increase in the succinylation at lysine 118 (K118su) of lactate dehydrogenase A (LDHA), which plays a role in increasing LDH activity. As a substrate of SIRT5, LDHA-K118su significantly increased the migration and invasion of PCa cells and LDH activity in PCa patients. This study reveals the reduction of SIRT5 protein expression and LDHA-K118su as a novel mechanism involved in PCa progression, which could serve as a new target to prevent CPRC progression for PCa treatment.
Measuring the impact of malaria infection on indicators of iron and vitamin A status: a systematic literature review and meta-analysis
Authors: Sandalinas Fanny , Filteau Suzanne , Joy Edward J. M. , Segovia de la Revilla Lucia , MacDougall Amy , Hopkins Heidi 
Published: March 2022

AbstractInflammation and infections such as malaria affect estimates of micronutrient status. Medline, Embase, Web of Science, Scopus and the Cochrane library were searched to identify studies reporting mean concentrations of ferritin, hepcidin, retinol or retinol binding protein in individuals with asymptomatic or clinical malaria and healthy controls. Study quality was assessed using the US National Institute of Health tool. Random effects meta-analyses were used to generate summary mean differences. In total, forty-four studies were included. Mean ferritin concentrations were elevated by: 28·2 µg/l (95 % CI 15·6, 40·9) in children with asymptomatic malaria; 28·5 µg/l (95 % CI 8·1, 48·8) in adults with asymptomatic malaria; and 366 µg/l (95 % CI 162, 570) in children with clinical malaria compared with individuals without malaria infection. Mean hepcidin concentrations were elevated by 1·52 nmol/l (95 % CI 0·92, 2·11) in children with asymptomatic malaria. Mean retinol concentrations were reduced by: 0·11 µmol/l (95 % CI −0·22, −0·01) in children with asymptomatic malaria; 0·43 µmol/l (95 % CI −0·71, −0·16) in children with clinical malaria and 0·73 µmol/l (95 % CI −1·11, −0·36) in adults with clinical malaria. Most of these results were stable in sensitivity analyses. In children with clinical malaria and pregnant women, difference in ferritin concentrations were greater in areas with higher transmission intensity. We conclude that biomarkers of iron and vitamin A status should be statistically adjusted for malaria and the severity of infection. Several studies analysing asymptomatic infections reported elevated ferritin concentrations without noticeable elevation of inflammation markers, indicating a need to adjust for malaria status in addition to inflammation adjustments.
Sex and Gender Equity in Prehospital Electrocardiogram Acquisition
Authors: McDonald Neil , Little Nicola , Grierson Rob , Weldon Erin 
Published: March 2022

AbstractIntroduction:Research in cardiac care has identified significant gender-based differences across many outcomes. Women with heart disease are less likely both to be diagnosed and to receive standard care. Gender-based disparities in the prehospital setting are under-researched, but they were found to exist within rates of 12-lead electrocardiogram (ECG) acquisition within one urban Emergency Medical Services (EMS) agency.Study Objective:This study evaluates the quality improvement (QI) initiative that was implemented in that agency to raise overall rates of 12-lead ECG acquisition and reduce the gap in acquisition rates between men and women.Methods:This QI project included two interventions: revised indications for 12-lead acquisition, and training that highlighted sex- and gender-based differences relevant to patient care. To evaluate this project, a retrospective database review identified all patient contacts that potentially involved cardiac assessment over 18 months. The primary outcome was the rate of 12-lead acquisition among patients with qualifying complaints. This was assessed by mean rates of acquisition in before and after periods, as well as segmented regression in an interrupted time series. Secondary outcomes included differences in rates of 12-lead acquisition, both overall and in individual complaint categories, each compared between men/women and before/after the interventions.Results:Among patients with qualifying complaints, the mean rate of 12-lead acquisition in the lead-in period was 22.5% (95% CI, 21.8% - 23.2%) with no discernible trend. The protocol change and training were each associated with a significant absolute level increase in the acquisition rate: 2.09% (95% CI, 0.21% - 4.0%; P = .03) and 3.2% (95% CI, 1.18% - 5.22%; P = .003), respectively. When compared by gender and time period, women received fewer 12-leads than men overall, and more 12-leads were acquired after the interventions than before. There were also significant interactions between gender and period, both overall (2.8%; 95% CI, 1.9% - 3.6%; P < .0001) and in all complaint categories except falls and heart problems.Conclusion:This QI project resulted in an increase in 12-leads acquired. Pre-existing gaps in rates of acquisition between men and women were reduced but did not disappear. On-going research is examining the reasons behind these differences from the perspective of prehospital providers.
A Model for Positive Corona Inception From Charged Ellipsoidal Thundercloud Hydrometeors
Authors: Peeters S. A. , Mirpour S. , Köhn C. , Nijdam S. 
Published: March 2022

AbstractLightning is observed to incept in thundercloud electric fields below the threshold value Ek for discharge initiation. To explain this, the local enhancement of the electric field by hydrometeors is considered. The conditions for the onset of positive corona discharges are studied in air for ellipsoidal geometries. A hydrometeor is simulated as an individual charged conductor in zero ambient field; there is only a field generated by the charge on the hydrometeor surface. By doing so, the feasibility of corona inception from ellipsoidal hydrometeors can be formulated based on the self‐sustaining condition of electron avalanches. For hydrometeor dimensions of a few millimeters and typical thundercloud pressure, values above 2.4 Ek are found for the onset electric field at the tip of the ellipsoid. From simulations the required ambient electric field for corona onset from an uncharged hydrometeor can then be derived. This results in values between 0.07 Ek and 0.8 Ek for semiaxes aspect ratios between 0.01 and 1. The charge required on the hydrometeor surface for corona onset for typical pressures is minimum for semiaxes aspect ratios increasing from 0.12 to 0.22 for decreasing hydrometeor volume from 42 to 4.2 mm3. For representative simulated hydrometeors, onset charges are between 495 and 2,430 pC. For a hydrometeor volume of 4.2 mm3 results are comparable to measured precipitation charges for aspect ratios between 0.16 and 0.32. From the results it is concluded that corona onset from ellipsoidal hydrometeors of a realistic volume can be achieved in thundercloud conditions for certain aspect ratios.
Response of Flow and Saltating Particle Characteristics to Bed Roughness and Particle Spatial Density
Authors: Zhao Chenwei , Ouro Pablo , Stoesser Thorsten , Dey Subhasish , Fang Hongwei 
Published: March 2022

AbstractWith the goal to explore the effects of natural bed roughness on bedload transport, numerical simulations of flow and particle saltation are carried out with varying bed roughness and particle spatial density. A combination of Eulerian and Lagrangian point‐particle methods is applied to solve the equations of motion of the fluid and the particles within the large‐eddy simulation framework. Flows over smooth and rough beds with four particle densities are considered. As the bed roughness increases, there is a leftward shift of the double‐averaged streamwise velocity profiles against dimensionless vertical distance, which is scaled with the bed roughness height, an upward shift of the peak values of double‐averaged Reynolds stresses, and a fragmentation and disappearance of coherent structures in the form of high‐speed and low‐speed near‐bed streaks. These observations are consistent with those of previous studies. As the bed roughness increases, the mean resting time of saltating particles increases, however the particles' saltation length, velocity, and angular velocity decrease, while their saltation height remains almost unchanged. Saltation height, saltation length, particle angular velocity, and resting time exhibit linear, gamma, normal, and exponential distributions, respectively. Further, as the bed roughness increases, the kurtosis and skewness of some particle parameters vary, and the particle velocity shifts from a symmetrical normal to an asymmetrical gamma distribution.
Liquid biopsy and non-small cell lung cancer: are we looking at the tip of the iceberg?
Authors: Bonanno Laura , Dal Maso Alessandro , Pavan Alberto , Zulato Elisabetta , Calvetti Lorenzo , Pasello Giulia , Guarneri Valentina , Conte PierFranco , Indraccolo Stefano 
Published: March 2022

AbstractThe possibility to analyse the tumour genetic material shed in the blood is undoubtedly one of the main achievements of translational research in the latest years. In the modern clinical management of advanced non-small cell lung cancer, molecular characterisation plays an essential role. In parallel, immunotherapy is widely employed, but reliable predictive markers are not available yet. Liquid biopsy has the potential to face the two issues and to increase its role in advanced NSCLC in the next future. The aim of this review is to summarise the main clinical applications of liquid biopsy in advanced non-small cell lung cancer, underlining both its potential and limitations from a clinically driven perspective.
Bone marrow-independent adventitial macrophage progenitor cells contribute to angiogenesis
Authors: Kleefeldt Florian , Upcin Berin , Bömmel Heike , Schulz Christian , Eckner Georg , Allmanritter Jan , Bauer Jochen , Braunger Barbara , Rueckschloss Uwe , Ergün Süleyman 
Published: March 2022

AbstractPathological angiogenesis promotes tumor growth, metastasis, and atherosclerotic plaque rupture. Macrophages are key players in these processes. However, whether these macrophages differentiate from bone marrow-derived monocytes or from local vascular wall-resident stem and progenitor cells (VW-SCs) is an unresolved issue of angiogenesis. To answer this question, we analyzed vascular sprouting and alterations in aortic cell populations in mouse aortic ring assays (ARA). ARA culture leads to the generation of large numbers of macrophages, especially within the aortic adventitia. Using immunohistochemical fate-mapping and genetic in vivo-labeling approaches we show that 60% of these macrophages differentiate from bone marrow-independent Ly6c+/Sca-1+ adventitial progenitor cells. Analysis of the NCX−/− mouse model that genetically lacks embryonic circulation and yolk sac perfusion indicates that at least some of those progenitor cells arise yolk sac-independent. Macrophages represent the main source of VEGF in ARA that vice versa promotes the generation of additional macrophages thereby creating a pro-angiogenetic feedforward loop. Additionally, macrophage-derived VEGF activates CD34+ progenitor cells within the adventitial vasculogenic zone to differentiate into CD31+ endothelial cells. Consequently, depletion of macrophages and VEGFR2 antagonism drastically reduce vascular sprouting activity in ARA. In summary, we show that angiogenic activation induces differentiation of macrophages from bone marrow-derived as well as from bone marrow-independent VW-SCs. The latter ones are at least partially yolk sac-independent, too. Those VW-SC-derived macrophages critically contribute to angiogenesis, making them an attractive target to interfere with pathological angiogenesis in cancer and atherosclerosis as well as with regenerative angiogenesis in ischemic cardiovascular disorders.
Participant experiences of genome sequencing for rare diseases in the 100,000 Genomes Project: a mixed methods study
Authors: Peter Michelle , Hammond Jennifer , Sanderson Saskia C. , Gurasashvili Jana , Hunter Amy , Searle Beverly , Patch Christine , Chitty Lyn S. , Hill Melissa , Lewis Celine 
Published: March 2022

AbstractIn this mixed methods study, a survey and in-depth interviews were used to explore whether decision regret and the psychological impact of receiving genome sequencing (GS) results differed between parents and patients, and between those who received a genetic diagnosis and those who did not. Participants (n = 77) completed a survey that included the Decisional Regret Scale (DRS) and an adaptation of the Multidimensional Impact of Cancer Risk Assessment (MICRA) at least 12 months after consenting for GS for rare disease diagnosis in the 100,000 Genomes Project. Survey participants were invited to take part in an interview and 39 agreed; 12 with a diagnosis, 5 with variants of uncertain significance, and 19 with no pathogenic findings identified. Both survey and interview findings indicated that decision regret was low. DRS scores revealed no differences in levels of regret between parents and patients, or between those with a diagnosis and those without. Though MICRA scores indicated minimal evidence of negative psychological impacts of receiving GS results, subscale analysis revealed greater distress and uncertainty for parents compared to patients. Receiving a diagnosis was found not to influence MICRA scores, supporting interview findings of both positive and negative emotional and psychological impacts irrespective of a genetic diagnosis. Our findings have implications for policy and practice as GS is integrated into the UK and worldwide; notably, that expectation-setting is critical when offering GS, and that post-test counselling is important regardless of the GS result received, with parents perhaps needing additional emotional support.
Superlinear scaling of riverine biogeochemical function with watershed size
Authors: Wollheim Wilfred M. , Harms Tamara K. , Robison Andrew L. , Koenig Lauren E. , Helton Ashley M. , Song Chao , Bowden William B. , Finlay Jacques C. 
Published: March 2022

AbstractRiver networks regulate carbon and nutrient exchange between continents, atmosphere, and oceans. However, contributions of riverine processing are poorly constrained at continental scales. Scaling relationships of cumulative biogeochemical function with watershed size (allometric scaling) provide an approach for quantifying the contributions of fluvial networks in the Earth system. Here we show that allometric scaling of cumulative riverine function with watershed area ranges from linear to superlinear, with scaling exponents constrained by network shape, hydrological conditions, and biogeochemical process rates. Allometric scaling is superlinear for processes that are largely independent of substrate concentration (e.g., gross primary production) due to superlinear scaling of river network surface area with watershed area. Allometric scaling for typically substrate-limited processes (e.g., denitrification) is linear in river networks with high biogeochemical activity or low river discharge but becomes increasingly superlinear under lower biogeochemical activity or high discharge, conditions that are widely prevalent in river networks. The frequent occurrence of superlinear scaling indicates that biogeochemical activity in large rivers contributes disproportionately to the function of river networks in the Earth system.
Deep learning shows declining groundwater levels in Germany until 2100 due to climate change
Authors: Wunsch Andreas , Liesch Tanja , Broda Stefan 
Published: March 2022

AbstractIn this study we investigate how climate change will directly influence the groundwater resources in Germany during the 21st century. We apply a machine learning groundwater level prediction approach based on convolutional neural networks to 118 sites well distributed over Germany to assess the groundwater level development under different RCP scenarios (2.6, 4.5, 8.5). We consider only direct meteorological inputs, while highly uncertain anthropogenic factors such as groundwater extractions are excluded. While less pronounced and fewer significant trends can be found under RCP2.6 and RCP4.5, we detect significantly declining trends of groundwater levels for most of the sites under RCP8.5, revealing a spatial pattern of stronger decreases, especially in the northern and eastern part of Germany, emphasizing already existing decreasing trends in these regions. We can further show an increased variability and longer periods of low groundwater levels during the annual cycle towards the end of the century.
Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43
Authors: Garcia Morato Jorge , Hans Friederike , von Zweydorf Felix , Feederle Regina , Elsässer Simon J. , Skodras Angelos A. , Gloeckner Christian Johannes , Buratti Emanuele , Neumann Manuela , Kahle Philipp J. 
Published: March 2022

AbstractTrans-activation response DNA-binding protein of 43  kDa (TDP-43) regulates RNA processing and forms neuropathological aggregates in patients with amyotrophic lateral sclerosis and frontotemporal lobar degeneration. Investigating TDP-43 post-translational modifications, we discovered that K84 acetylation reduced nuclear import whereas K136 acetylation impaired RNA binding and splicing capabilities of TDP-43. Such failure of RNA interaction triggered TDP-43 phase separation mediated by the C-terminal low complexity domain, leading to the formation of insoluble aggregates with pathologically phosphorylated and ubiquitinated TDP-43. Introduction of acetyl-lysine at the identified sites via amber suppression confirmed the results from site-directed mutagenesis. K84-acetylated TDP-43 showed cytoplasmic mislocalization, and the aggregation propensity of K136-acetylated TDP-43 was confirmed. We generated antibodies selective for TDP-43 acetylated at these lysines, and found that sirtuin-1 can potently deacetylate K136-acetylated TDP-43 and reduce its aggregation propensity. Thus, distinct lysine acetylations modulate nuclear import, RNA binding and phase separation of TDP-43, suggesting regulatory mechanisms for TDP-43 pathogenesis.
Antiferroelectric negative capacitance from a structural phase transition in zirconia
Authors: Hoffmann Michael , Wang Zheng , Tasneem Nujhat , Zubair Ahmad , Ravindran Prasanna Venkatesan , Tian Mengkun , Gaskell Anthony Arthur , Triyoso Dina , Consiglio Steven , Tapily Kandabara , Clark Robert , Hur Jae , Pentapati Sai Surya Kiran , Lim Sung Kyu , Dopita Milan , Yu Shimeng , Chern Winston , Kacher Josh , Reyes-Lillo Sebastian E. , Antoniadis Dimitri , Ravichandran Jayakanth , Slesazeck Stefan , Mikolajick Thomas , Khan Asif Islam 
Published: March 2022

AbstractCrystalline materials with broken inversion symmetry can exhibit a spontaneous electric polarization, which originates from a microscopic electric dipole moment. Long-range polar or anti-polar order of such permanent dipoles gives rise to ferroelectricity or antiferroelectricity, respectively. However, the recently discovered antiferroelectrics of fluorite structure (HfO2 and ZrO2) are different: A non-polar phase transforms into a polar phase by spontaneous inversion symmetry breaking upon the application of an electric field. Here, we show that this structural transition in antiferroelectric ZrO2 gives rise to a negative capacitance, which is promising for overcoming the fundamental limits of energy efficiency in electronics. Our findings provide insight into the thermodynamically forbidden region of the antiferroelectric transition in ZrO2 and extend the concept of negative capacitance beyond ferroelectricity. This shows that negative capacitance is a more general phenomenon than previously thought and can be expected in a much broader range of materials exhibiting structural phase transitions.