bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analyse micreconometrique the Determinants of socio-economic in the use of basic health services in Senegal
Authors: Kâ Mamadou Moustapha 
Published: May 2022

Abstract
Background: In spite of social and economic interventions, state health policies have not yet succeeded in providing households in poor and low-income areas with better chances of survival and better chances of using health services. Health services are part of a larger structure called the health system. The health system is made up of all the elements that determine the health status of a population. The starting point of the health system is the population and more specifically the identification of the health needs of that population.The objective of this paper is to assess the influence of the different factors determining the use of health services in Senegal.Health inequities undermine the lives and futures of all vulnerable groups by creating systems of social injustice.Methods: Since the outcome variable was a dichotomous variable, a discrete choice model was employed to show how the explanatory variables correlated with the outcome variable. Specifically, the binary logistic regression was employed given that this technique is more appropriate for dichotomous variables. A key assumption underlying the binary logistic regression model is that the dependent variable should be dichotomous in nature and the data should not have any outlier.Results: The results showed that the main finding of the study is that low educational attainment has significant negative effects on health service utilization. In fact, educational attainment is one of the key determinants of health.Household economic status, which was grouped into four representative modalities form the three explanatory variables, and each of the individuals classified according to these modalities through their income strongly influences the decision to go to the hospital.JEL classification : H51, H52, H53, H75
Impact of Resettlement programme on Land Use and Land Cover Dynamics: The Case of Selected Resettlement Villages in Abobo District, Gambella, Southwest Ethiopia
Authors: ochocho Abraham , wubela berhanu 
Published: May 2022

Abstract
Most of the studies solely focused on specific resettlement sites neglecting Abobo woreda which is one of the resettlement program areas in Ethiopia. No study has particularly investigated the impact of the program on land use and its cover dynamics in Abobo, where the significant numbers of households resettled. This study; therefore, aimed at to fill the research gap that was missed intending to analyse the impact of resettlement on land use cover dynamics in Abobo district, Western Ethiop. To conduct this study three periods of images of Landsat 4TM, 1982, Landsat 5TM1998 and Landsat 8ETM+ 2014 were used. Google Earth has been used for ground verification. Data was analysed by using Arcgis10.1, ENVI 4.7 and Microsoft excels 2007 software. The study detected conversion type of changes. The result of the study depicted that continuous expansion of farmland, settlement as well as grassland increase with the rate of + 21.58ha, + 20.46ha and + 19.36ha per year at the expanse of forest and woodland area. Further, both forest and woodland are diminished with a rate of -34.22ha and 27.20ha per year respectively. In addition, forestland and woodland are the two most shrinking land cover types which imply the loss of vegetation due to expansion of farmland and settlement. Therefore, resettlement should not be taken as the best alternative to minimize drought and famine or ensure food security, to guarantee effective food security in the long run. Other Strategies such as in addition to crop farming, the promotion of livestock production and off-farm employment; natural resource conservation and rehabilitation should be adopted in the district.
GsMYB7 Encoding a R2R3-type MYB Transcription Factor Enhances the Tolerance to Aluminum Stress in Soybean (Glycine Max.L)
Authors: hongjie wang , Yin Xiangli , Du Dan , Liang Zhongyi , Han Zhenzhen , Nian Hai , Ma Qibin 
Published: May 2022

Abstract
Background
TMYB transcription factor (TF) is one of the largest families of TFs in plants and play essential roles in plant growth and development, and is involved in responses to biological and abiotic stress. However, there are limited reports on GsMYB7 gene in soybean under aluminum acid stress, and its regulatory mechanism remains unclear.
Results
The GsMYB7 protein is localized in the nucleus and has transcriptional activation ability. Quantitative real-time PCR (qRT-PCR) results showed that GsMYB7 held a constitutive expression pattern rich in roots. When AlCl3 concentration was 25 µM, the total root surface area (SA) of GsMYB7 transgenic lines were 34.97% higher than that of wild-type. While the accumulation of Al3+ in root tip of transgenic plants after aluminum treatment was 17.39% lower than that of wild-type Huachun 6 (HC6). RNA-sequencing analysis indicated that over 1181 genes were regulated by GsMYB7 and aluminum stress. Among all the regulated genes, the expression levels of glutathione peroxidase, protein kinase, cytochrome and other genes in the transgenic lines were significantly higher than those in wild type by acidic aluminum stress. The bioinformatics and qRT-PCR results showed that 9 candidate genes were induced under the treatments of acidic aluminum stress which were indirectly and/or directly regulated by GsMYB7. After AlCl3 treatments, the transcripts of these genes in GsMYB7 transgenic seedlings were significantly higher than those of wide-type HC6.
Conclusions
The results suggested that GsMYB7 may enhance soybean tolerance to acidic aluminum stress by regulating the downstream genes.
Seasonal dynamics of the mats formed by conjugating algae of the genus Zygnema (Zygnematophyceae) in Austrian Alps
Authors: Trumhová Kateřina , Klimešová Vanda , Pichrtová Martina 
Published: May 2022

Abstract
Filamentous green algae of the genus Zygnema are an essential part of hydro-terrestrial ecosystems and stream waters. Despite several studies on their resistance to natural stresses, little is known about the composition of their assemblages and the changes they undergo over time. Two sites at altitudes above 2200 m a.s.l. in the Austrian Alps were selected for a two-year observation period and sampled five times. Molecular phylogenetic analysis of the 152 isolated strains was performed based on the rbcL and trnG sequences. Seven genotypes were found at these sites, but their abundance varied throughout the seasons. The site with a more stable water regime also had a more stable representation of genotypes, in contrast to the site with fluctuating water availability. The mats formed resistant pre-akinetes at the end of the season with reduced photosynthetic activity. Contrary to expectations, the mats were not exposed to extremely cold temperatures in winter due to snow cover. Some genotypes have been previously observed at this site, indicating that the population composition is stable. This work highlights the importance of resistant pre-akinetes in surviving winter conditions, the ability of algae to re-establish mats, and the need to address the hidden diversity of the genus Zygnema.
Construction of a multi-bioresponsive drug delivery system for a hyaluronic acid- geraniol polymeric prodrug and evaluation of its antitumor effect
Authors: Xia Yifan , Tian Xue , Cui Jie , Zhang Xin , Yang Qian , Zhao Tingkui , Lin Yuxia , Cen Juan , Duan Shaofeng 
Published: May 2022

Abstract
Geraniol (GER), widely found in nature, exerts effective anti-tumoral activity against various malignant tumors. However, its low water solubility and poor bioavailability have greatly hindered its translation to the clinic. To overcome these drawbacks, a simple redox-sensitive GER hyaluronic acid (HA) polymer prodrug was synthesized by conjugating GER to HA using a disulfide bond. The synthesized prodrug (HSSG) as a molecular structural motif could self-assembly into simple yet multifunctional nanoparticles (HSSG NPs) in aqueous solution. The HSSG NPs displayed an average diameter of ∼110 nm with a uniformly spherical shape and maintained stability in different physiological media. Moreover, these NPs showed accelerated drug release rates when they were exposed to buffers that mimicked the multi-hallmarks in the tumor microenvironment (pH/glutathione/hyaluronidase). Furthermore, the results of fluorescent microscope and flow cytometry verified that the nanosystems were selectively uptaken by human hepatocellular carcinoma cell lines HepG2 and Huh7 via CD44 receptor-mediated internalization through the targeting. In addition, in the H22 tumor-bearing mice as the model, HSSG NPs could accumulate within tumor sites for a longer period. Notably, in vitro and in vivo antitumor results demonstrated that HSSG NPs significantly promoted the death of cancer cells and enhanced the inhibition of tumor growth while reducing the toxicity as compared to GER and HCCG NPs. Therefore, the HSSG NPs as the simple and efficient platform have great potential in tumor-targeting drug delivery and therapy.
Study of microstructure and mechanical behaviour of Ti2AlC reinforced hypereutectic Al-16Si matrix composites fabricated by induction melting
Authors: Trinath K , Aepuru Radhamanohar , Biswas Ajay 
Published: May 2022

Abstract
The newly developed ternary-layered compound Ti2AlC reinforced Al-Si hyper-eutectic matrix composites successfully fabricated through a rapid heating rate of induction technique. The microstructure and, tensile performance, Micro Vickers hardness were systematically investigated. The Al-Ti2AlC system composites with varying Ti2AlC weight percentages undergone to investigate microstructure and mechanical strength. According to SEM data, the presence of the in-situ TiAl3 phase can significantly impact the interface bonding condition. After reinforcement with MAX phase particles, the ductility of the Al-Si hyper-eutectic alloy was increased by roughly 33%. According to morphological evaluation, casted samples exhibited intermetallic compounds, interfaces, and microcracks.
In vivo characterization and analysis of glioblastoma at different stages using multiscale photoacoustic molecular imaging
Authors: Zhang Jinde , Sun Xiang , Li Honghui , Ma Haosong , Duan Fei , Wu Zhiyou , Zhu Bowen , Chen Ronghe , Nie Liming 
Published: May 2022

Abstract
Simultaneous spatio-temporal description of tumor microvasculature, blood-brain barrier, and immune activity is pivotal to understanding the evolution mechanisms of highly aggressive glioblastoma, one of the most common primary brain tumors in adults. However, the existing intravital imaging modalities are still difficult to achieve it in one step. Here, we present a dual-scale multi-wavelength photoacoustic imaging approach cooperative with/without unique optical dyes to overcome this dilemma. Label-free photoacoustic imaging depicted the multiple heterogeneous features of neovascularization in tumor progression. In combination with classic Evans blue assay, the microelectromechanical system based photoacoustic microscopy enabled dynamic quantification of BBB dysfunction. Concurrently, using self-fabricated targeted protein probe (αCD11b-HSA@A1094) for tumor-associated myeloid cells, unparalleled imaging contrast of cells infiltration associated with tumor progression was visualized by differential photoacoustic imaging in the second near-infrared window at dual scale. Our photoacoustic imaging approach has great potential for tumor-microenvironment visualization to systematically reveal the tumor infiltration, heterogeneity, and metastasis in intracranial tumors.
Disposable Electrocatalytic Sensor for Whole Blood NADH Monitoring
Authors: Lee JuKyung , Suh Han NA , Ahn Saeyoung , Park Hyebin , Kim Hyung Jin , Kim SangHee 
Published: May 2022

Abstract
In the study, the preparation and electrochemical characterization of screen-printed electrodes (SPE) modified with 4’-mercapto-N-phenylquinone diamine (NPQD) and their behavior as electrocatalysts toward the oxidation of NADH with high stability are described. In particular, NPQD on the SPE substrate was deposited via electro-polymerization. Thus, the modified electrode exhibited a limit of detection (LOD) corresponding to 3.5 µM LOD with a sensitivity corresponding to 0.0076 ± 0.0006 µM/µA in the mouse serum. In all cases, NADH oxidation occurred at potentials approximately corresponding to 0.7 V vs Ag/AgCl electrode, and this indicates a decrease in the overpotential. We used the observations as a point of departure and developed an NADH biosensor based on the electrocatalytic oxidation of NADH. The applicability of the NADH sensor was demonstrated for the first time in 1) selectivity/sensitivity test via complex electrolyte as cell culture medium and 2) inflammatory and fibrotic responses by polyhexamethylene guanidine phosphate via NADH sensing in mouse serum.
Alpha-emitter radium-223 mediates STING-dependent pyroptosis to trigger robust tumor immunogenicity
Authors: Yang Mengdie , Cheng Chao , Zhang Jiajia , Zuo Changjing , Zhu Mengqin , Zhang Xiaoyi , Yin Yuzhen , Zhang Yu , Qin Shanshan , Zhang Han , Fan Xin , Yu Fei 
Published: May 2022

Abstract
Purpose Radium-223 (223Ra) is the first-in-class alpha-emitter to mediate tumor eradication, which radiation emitted from alpha-emitter are routinely regarded can reduce tumor burden by directly cleaving double-strand DNA and killing tumor cells. However, the immunogenic characteristics and various cell death modalities triggered by alpha-emitter are remain unclear.MethodsWe evaluated biological effects of 223Ra in cancer cell lines and mice models by biological endpoints. Concurrently, we explored immunogenicity and cell death modalities triggered by 223Ra and excavated its mechanisms of pyroptosis induction. Besides, synergistic effect with checkpoint blockade was also assessed.Results223Ra has efficient therapeutic antitumor effects, which examined by cell viability study, foci analysis for DNA damage, and tumor regression. We further demonstrated that irradiated cells released pro-inflammatory damage-associated molecular patterns (DAMPs), including surface-exposed calreticulin (CALR), released high mobility group protein B1 (HMGB1), and secreted heat shock protein 70 (HSP70), which actively shape tumor immunogenicity. Pyroptosis, an immunogenic cell death (ICD) accompanied by activation of inflammasome, was also found to play significant roles in the modulation of cancer progression under the therapeutic 223Ra. Mechanically, this effect relies on 223Ra-induced DNA damage, which activates the STING-mediated DNA sensing pathway, leading to NLRP3 inflammasome-dependent pyroptosis. When combined with anti-CD47 therapy, 223Ra can trigger robust antitumor effect. ConclusionThese findings highlight the tumor eradication effect of 223Ra, which exhibit both direct antitumor effects and indirect immune-stimulating capabilities. Importantly, STING/NLRP3 axis has a crucial role in pyroptosis under the therapeutic 223Ra, which may shed light on promising targets for therapeutic interventions.
WITHDRAWN: NS3TP1 promotes liver fibrosis by activating HSCs through TGFβ1/Smad3 and NF-κB/NLRP3 inflammasome signaling pathways
Authors: Shi Liu , Zhou Li , Han Ming , Zhang Yu , Zhang Yang , Yuan Xiaoxue , Lu Hongping , Wang Yun , Yang Xueliang , Liu Chen , Wang Jun , Liang Pu , Liu Shunai , Liu Xiaojing , Cheng Jun , Lin Shumei 
Published: May 2022

Abstract
The situation of liver fibrosis is still serious, but there is no effective treatment at present. Therefore, additional study on the mechanism of liver fibrosis is an urgent requirement. The present study aimed to elucidate the mechanism of NS3TP1 in liver fibrosis and the regulatory role of NS3TP1-SEP and calcitriol on NS3TP1. All experiments were performed in LX-2 cell lines and CCl4-induced C57BL/6 wild-type mice liver. NS3TP1 promoted differentiation, activation, and proliferation of hepatic stellate cells (HSCs) and upregulated liver fibrosis through TGFβ1/Smad3 and NF-κB signaling pathways. Both Smad3 and p65 bind to NS3TP1, and p65 upregulated the promoter of NS3TP1, while NS3TP1 upregulated the promoter of TGFβRI. ASDURF regulated the expression of NS3TP1. After overexpression of ASDURF, NS3TP1 and liver fibrosis-related genes were upregulated, whereas after interference, NS3TP1 and liver fibrosis-related genes were downregulated, besides, ASDURF and NS3TP1 had a binding effect and upregulated the promoter of NS3TP1. Calcitriol significantly inhibited CCl4-induced liver fibrosis in mice and regulated the differentiation, activation, and proliferation of HSCs. Furthermore, calcitriol regulated the activities of TGFβ1/Smad3 and NF-κB/NLRP3 inflammasome signaling pathways via downregulation of NS3TP1. These findings provided evidence for the role of NS3TP1 as a new promising therapeutic target in liver fibrosis.
Glucocorticoid Receptor Regulated KCNA5 Mediates Cell Proliferation in Human Fetal Membranes
Authors: Cunningham Sarah J , Barrera Alejandro , Sankaranarayanan Laavanya , Allen Terrence K , Reddy Timothy E 
Published: May 2022

Abstract
Background
Preterm birth is a major public health issue, affecting approximately 10% of pregnancies in the United States. A major identifiable cause of preterm birth is preterm premature rupture of membranes (PPROM). PPROM is a pregnancy complication in which the amnion and chorion weaken and rupture prior to 37 weeks of pregnancy and before contractions have begun. PPROM is responsible for 30–40% of preterm birth cases. Recently, PPROM has been closely linked to maternal stress, leading us to hypothesize that glucocorticoid signaling may contribute to PPROM.
Methods
We measured the gene expression effects of glucocorticoids in primary amnion cells using RNA-seq. KCNA5 emerged as a potential GR regulated gene. To measure the effects of KCNA5 on cell proliferation in primary amnion epithelial cells, we used siRNA to reduce KCNA5 expression and Cas9-based epigenome editing to increase KCNA5 expression in HEK293T cells; followed by cellular assays to measure effects of proliferation and apoptosis.
Results
KCNA5 knockdown significantly increased cell proliferation without appearing to impact apoptosis. CRISPR-mediated over expression of endogenous KCNA5 significantly decreased cell proliferation.
Conclusions
Glucocorticoid-mediated activation of KCNA5 contributes to decreased cell proliferation in cultured primary amnion epithelial cells. Decreases in amnion epithelial proliferation could impair the ability of these cells to repair microfractures in the membrane and lead to overall membrane weakening consistent with PPROM.
Epidemiology of Sexually Transmitted Infections: Trends among Patients Screened for Sexually Transmitted Infections in Rwandan Health Facilities 2014-2020
Authors: Makuza Jean Damascene , Soe Phyumar , Jeong Dahn , Nisingizwe Marie Paul , Dushimiyimana Donatha , Umutesi Justine , Nshimiyimana Ladislas , Maliza Clarisse , Serumondo Janvier , Remera Eric , Rwibasira Gallican Nshogoza , Tuyishime Albert , Riedel David J. 
Published: May 2022

Abstract
Background: Sexually Transmitted Infections (STIs) are of great global health concern. Currently, there are limited epidemiological data characterizing STIs in the general population in Rwanda. We assessed the epidemiology of STIs in different Rwandan regions from 2014-2020 among patients syndromically screened for STIs in all health facilities in Rwanda.Methods: This is a retrospective analysis of the trend of STIs epidemiology among screened patients at all health facilities in Rwanda using data from the Health Management Information System (HMIS) reporting. Adult patients (15 years and over) screened for STIs between July 2014 and June 2020 were included in the analysis. Outcomes of interest were the number of individuals screened for STIs and individuals diagnosed with at least one STI with a syndromic approach only or plus a test together.Results: Overall, the number of individuals screened for STIs over the study period was 5.3 million (M) in 2014-2015, 6.6 M in 2015-2016, 6.3 M in 2016-2017, 6.7M in 2017-2018, 6.2 M in 2018-2019, and 4.9 M in 2019-2020. There was a modest increase in the number of individuals diagnosed and treated for STIs from 139,357 in 2014-15 to 202,294 (45% increase) in 2019-2020. At the national level, the prevalence of STIs in Rwanda varied between 2.37% to 4.16% during the study period. Among the provinces, Kigali city had the highest prevalence for the whole 6 years ranging from 3.46% (95%CI: 3.41, 3.51) in 2014-2015 to 8.23% (95%CI: 8.15, 8.31). Conclusion: From 2014 to 2020, the number of patients screened for STIs in Rwanda did not vary much. However, the prevalence of STIs among screened patients increased considerably over time, which could be associated with public awareness and improved data recording. The highest prevalence of all STIs was observed in urban areas and near borders, and private clinics reported more cases, suggesting the need to improve awareness in these settings and increase confidentiality and trust in public health clinics.
A survival model for prognostic prediction based on ferropto-sis-associated genes and the association with immune infiltra-tion in Lung squamous cell carcinoma
Authors: Lu Yanyi , Cao Yunliang , Wang Yunan , Wu Mengjia , He Bo , Xu Junzhu , Su Zixuan , Hu Wei 
Published: May 2022

Abstract
Lung squamous cell carcinoma (LUSC) is the primary pathological type of lung cancer with a less favourable prognosis. This study attempts to construct a ferroptosis-associated signature associated with OS, which can predict the prognosis of LUSC and explore its relationship with immune in-filtration. A 5 ferroptosis-associated genes model was constructed by the LASSO-penalized regres-sion analysis to predict the prognosis of patients with LUSC in the TCGA database and validated in GEO and TCGA databases. Patients were stratified into high-risk and low-risk groups by median value of the risk scores, and the former prognosis was significantly worse (P<0.001). Additionally, we found a certain association between the two risk groups and immune infiltration through CIBERSORT. Meanwhile, the differentially expressed genes (DEGs) between normal and tumor tissue were used to perform functional analysis, which shows significant association with leukocyte transendothelial migration pathways in the TCGA cohort. Besides, immune cell infiltration analysis confirmed that M2 macrophages were significantly highly expressed in the high-risk group. Overall, the model successfully established by ferroptosis-associated genes suggests that ferroptosis may be related to immune infiltration in LUSC.