bluuebunny/crossref_metadata_2025_split huggingface dataset:
Climate drivers and temporal variation of Ixode ovatus abundance on a giant panda living in the wild
Authors: Fan Xueyang , Ma Rui , Yue Changjuan , Liu Jiabin , Yue Bisong , Yang Zhisong , Yang Wanjing , Li Yunli , Gu Jiang , Yan Xia , Qi Dunwu , Su Xiaoyan , Li Lin , Zhang Dongsheng , Zhang Hongwen , Bunker Daniel E. , Hou Rong , Liu Songrui 
Published: October 2022

Abstract
Background
Ticks and tick-borne diseases have negative impacts on the health of wild animals including endangered and vulnerable species. The giant panda (Ailuropoda melanoleuca), one of the iconic flagship species, is threatened by tick infestation as well. Ticks can not only cause anemia and immunosuppression, but also bacterial and viral disease of giant pandas. However, many previous studies about ticks on giant pandas were only limited in scope as case reports of ill or dead animals.
Methods
In this study, an investigation about ticks on a reintroduced giant panda at Daxiangling Reintroduction Base in Sichuan of China was conducted. Ticks were collected daily and identified from the ears of the giant panda from March to September in 2021. A linear model was used to test the correlation between daily tick abundance and climate factors.
Result
All ticks were identified as Ixodes ovatus. The daily average of tick population was 6.78 (95% CI: 6.17–7.39) including 1.79 daily males (95% CI: 1.61–1.98) and 4.99 for females (95% CI: 4.53–5.44). Tick abundance was significantly different among months. Tick abundance increased from March and reached the highest point in June and July, then it decreased until September. Results from linear model showed that the temperature positively correlated to tick abundance while air pressure had a negative correlation with tick abundance.
Conclusions
This study investigated tick species and abundance on a giant panda living in the natural environment for the first time, which provided important information for the conservation of giant pandas and other species sharing the same environment.
WITHDRAWN: External cavity quantum cascade laser based gas sensor for sulfur hexafluoride detection
Authors: Pan Xingyu , Zhang Yifan , Zeng Jiayu , He Tianbo , Li Jingsong 
Published: October 2022

Abstract
External cavity quantum cascade laser (ECQCL) is a ideal mid-infrared (MIR) spectral light source, which provides ultra wide wavelength tuning range for multiple trace gases sensing simultaneously. In this paper, a gas sensor system was developed by using a broadband tunable ECQCL and a direct absorption spectroscopy detection scheme with a short path absorption cell of 29.6 cm. For spectral signal detection, a cheap and miniaturized quartz crystal tuning fork (QCTF) based light detector was used for laser signal detection. The characteristics of the QCTF detector was theoretically simulated and experimentally observed. To demonstrate this sensing technique, sulfur hexafluoride (SF6) was selected as the analyte, which can be used as an effective indicator to identify fault types of gas insulated electrical equipments. Preliminary result indicated that a good agreement was obtained between experimentally observed data and reference spectra by according to NIST database and previous publication, and the gas sensor system shows a good linear response to SF6 gas concentration. Finally, Allan deviation analysis indicates that detection limits of 1.889 ppm for SF6 was obtained with a 1 s integration time, which can be further improved to ~ 0.384 ppm by averaging up to 131 s, respectively.
Explicit approximation of the invariant measure for SDDEs with the nonlinear diffusion term
Authors: Li Xiaoyue , Mao Xuerong , Song Guoting 
Published: October 2022

Abstract
To our knowledge, the existing measure approximation theory requires the diffusion term of the stochastic delay differential equations (SDDEs) to be globally Lipschitz continuous. Our work is to develop a new explicit numerical method for SDDEs with the nonlinear diffusion term and establish the measure approximation theory. Precisely, we construct a function-valued explicit truncated Euler-Maruyama segment process (TEMSP) and prove that it admits a unique ergodic numerical invariant measure. We also prove that the numerical invariant measure converges to the underlying one of SDDE in the Fortet-Mourier distance. Finally, we give an example and numerical simulations to support our theory.
The 100 most cited papers on thymoma: a bibliometric analysis
Authors: Liu Lei , Zhang Jiaqi , Wang Guige , Zhao Ke , Guo Chao , Huang Cheng , Li Shanqing , Chen Yeye 
Published: October 2022

Abstract
Objectives:This study aims to identify the 100 most cited research articles on thymoma.
Methods: The 100 most cited articles on thymoma were identified by searching the Web of Science database. The following important information of selected articles was extracted: author, journal, impact factor, type of article, year of publication, country, organization, and keyword.
Results: To our knowledge, this is the first bibliometric study on thymoma. The publication year of the top 100 most cited articles ranged from 1981 to 2018, and the number of citations ranged from 97 to 1182. Most of the included articles are original (75/100) and are mainly retrospective studies (52/75). The United States has the most published articles and citations, and the Annals of Thoracic Surgery is the most sourced journal (n=16). Through VOSviewer analysis, high-density keywords mainly come from thymic carcinoma/invasive thymoma management, immune-related diseases, and laboratory research.
Conclusions: Our study summarizes the top 100 most cited articles in the field of thymoma and conducts further analysis. The previous research in this field and possible future research directions are presented.
Automated Detection and Statistical Study of Solar Radio Spikes
Authors: Lv P. R. , Hou Y. C. , Feng S. W. , Du · Q.F. , Tan C. M. 
Published: October 2022

Abstract
The most typical observational features of solar radio spikes are their short duration and narrow bandwidth. For these characteristics, we have improved the YOLOv5s network model, by adding inclined bounding frames, and attention and feature fusion mechanism modules. The decimeter- and meterwavelength spikes observed by the Solar Broad-band Radio Spectrometer in Huairou and the spectrograph in the Chashan Solar Radio Observatory are used to carry out experiments, respectively. The results demonstrate that the AP value obtained by the improved network is 74%, which is almost 14% higher than the original network. The improved network detects 9709 (1379) decimeter- (meter-) wavelength spikes in two events with the durations, bandwidths, relative bandwidths, and frequency-drift rates. The spikes at decimeter and meter wavelengths are categorized again based on their frequency-drift rates, such as, positive, negative, and no measurable frequency-drift rates, respectively. We have carried out a statistical study on these categorized spikes. An interesting finding is that the distribution of relative bandwidths at decimeter wavelengths is changed after the classification. The distribution is asymmetric without the classification. However, after the classification the positive, negative, and no measurable frequency-drift spikes show a symmetrical distribution. These statistical results and findings provide constraints on the formation of solar radio spikes.
CORALIS, an R package for ncRNA-target enrichment analysis based on experimentally validated interactions
Authors: Valle-Millares Daniel , Briz Verónica , Fernández-Rodríguez Amanda 
Published: October 2022

Abstract
Non-coding RNAs are key regulatory molecules that govern a myriad of biological process by interacting and modulating the expression of their target protein-coding genes. The combination of RNA large-scale sequencing and computational analyses has revealed the existence of a vast diversity of RNA species with essential roles in biological functions. However, dealing with the huge network of interactions has become a challenge and the urgent need for new computational tools has arisen. We present CORALIS, an R package for the statistical analysis and visualization of experimentally validated interactions between non-coding RNAs and their target genes (ncRNA-mRNA). So far, CORALIS supports miRNA, lncRNA, snRNA and snoRNA target enrichment analysis for several species such as Homo sapiens, and other human-model species such as Rattus norvegicus, Caenorhabditis elegans and Drosophila melanogaster, among others. Statistical tests (hypergeometric distribution test) are performed to estimate enriched target genes for non-coding RNAs defined by the user, and visualization tools allows to easily analyze the results. In conclusion, CORALIS allows identifying enriched target genes given a list of non-coding RNAs. CORALIS is freely accessible at https://github.com/Daniel-VM/CORALIS.
Magnetocaloric Effect (MCE) of a Quantum pseudo-dot
Authors: Donfack B. , Nguepnang J. V. , Nguemassong S. C.N. , Temdie L. , Manfouo F. , Tchida V. D. , Magouwo L. D. , Kamdem E. F. , Kenfack C. S. , Fotue A . J. 
Published: October 2022

Abstract
We investigate the Magnetocaloric Effect (MCE) of a quantum pseudodt taking into account the effect of spin orbit interaction (SOI). The entropy change and internal energy change been derived using the Tsallis formalism. we observed that the SOI effect and external parameters strongly affect the entropy change and internal energy change of a quantum speudodot. Knowin the magneto caloric effect being strongly related to the magnetic dipol, the effect of external magnetic field and the SOI considerably affect the entropy change as welle analytically than numerically. Also, it is found that the magneto caloric effect is sensitive to the extensivity parameter q.
Crack growth in viscoelastic media with large strains: further results and validation of nonlinear theory for rubber
Authors: Schapery R. A. 
Published: October 2022

Abstract
This paper is a continuation of recent publications on crack growth in viscoelastic media. It provides further theoretical results that enable prediction of crack opening displacement for comparison with experimental data in the region of the singularity . Additionally, the analysis shows that local material rotation in the singularity, where strains are very large, has no significant effect on the predicted stresses and deformation in the singularity. This is true despite the theory’s frame- dependence.
Atmospheric turbulent effects on the propagation properties of a General Model vortex Higher-order cosh-Gaussian beam
Authors: Ebrahim Ahmed Abdulrab Ali , Swillam Mohamed A , Belafhal Abdelmajid 
Published: October 2022

Abstract
The propagation properties for a General Model vortex Higher-order cosh-Gaussian beam (GMvHchGB) propagating in a turbulent atmosphere studied in detail. Based on the Huygens-Fresnel diffraction principle, the analytical formula of the intensity evolution for the considered beam traveling in turbulent atmosphere is derived at various propagation distances. The derived equation provides a general convenient procedure to describe the propagation characteristics to some particular beams travelling through free space/atmospheric turbulence such as a fundamental Gaussian, Cosh-Gaussian, vortex Cosh-Gaussian and higher-order Cosh-Gaussian beams. The impact of the incident parameters as Gaussian waist, Cosh parameter, wavelength, hollowness and order of the beam are numerically verified. Our study proven that the beams maintain their intensity focused over long propagation distances. Therefore, they will be useful in long-distance free-space optical communication applications.
A non-Gaussian Regularization of Graviton: Antipodal Duality between the Higgs and 3-Gluons
Authors: Maalouf Hani W. 
Published: October 2022

Abstract
A renormalization for the Graviton, with regularizing factors, based on the Anti-podal Symmetry is
seen to provide the link between the Higgs and 3-gluons.
Physically, such resolves, their so far abstract connection, via the MHV vertices.
The resolution involved a small variation of the Lagrangian cosmological term constant, under a
scalable Gauss-Bonnet Gravity.
Research on the release relationship between dissolved oxygen (DO) and total dissolved gas (TDG) in standing water
Authors: Yao Yuanbo , Yang Huixia , Wang Yunyun 
Published: October 2022

Abstract
Supersaturation of dissolved oxygen (DO) and total dissolved gas (TDG) is generated by high dam discharge, excess oxygen production in photosynthesis and increasing temperature in water, which may directly lead to fish suffering from ‘gas bubble disease’ or death. In this paper, under a series of experimental aeration conditions in standing water, it was concluded that aeration had a positively promoting effect on releases of supersaturated DO and TDG, while aeration aperture and aeration depth had inhibitory effects on them. For single factor analysis, aeration had the greatest effect on the release of DO and TDG, the second effect on DO was that of aeration depth and the smallest effect was that of aeration aperture, but the second effect on TDG was that of aeration aperture and the smallest effect was that of aeration depth. Most importantly, the release coefficient of DO was greater than that of TDG, and a quantitative relationship between the release coefficient of DO and TDG and aeration conditions, respectively, was established. An exponential function relationship of the release coefficients of DO and TDG was also established. The results of the research have important guiding significance and theoretical value for reducing the harm caused by supersaturated DO and TDG.
Upregulation of Connexins in the Rat Hippocampal and Cortical Neurons
Following Blockade of NMDA Receptors During Postnatal Development
Authors: Kourosh-Arami Masoumeh , Soleimani Mansoureh , Joghataei Mohammad Taghi , Mosleh Masoumeh , Hayat Parisa , Komaki Alireza 
Published: October 2022

Background:
Interneural gap junctional coupling represents neural development that
decreases during the postnatal period. The decrease of gap junction function coincides with the main
period of chemical synapse creation and increment of synaptic activity during postnatal weeks 1 to 3.

Methods:
Here, we have assessed the role of chemical synapses on connexin (Cx) expression in
neurons and glial cells of hippocampal and cortical neurons. We characterized the impact of NMDA
receptors blockade on the expression of Cx36 and Cx43 proteins by western blot analysis in postnatal
day (PND)14 and PND28. MK801 was injected subcutaneously from the first day of birth until 14 or 28
days, depending on the experimental groups. Saline was injected in the same volumes in the control
group.

Results:
Early postnatal blockade of the NMDA subtype of glutamate receptors by the non-competitive
antagonist dizocilpine maleate (MK801) arrested the developmental reduction in gap junctions during
the initial postnatal weeks. Expression of Cx43 declined in PND28 compared to PND14 in visual cortex
(VC) neurons. Also, we found that the expression of Cx36 and Cx43 augmented in the rats' VC in
PND28 following the blockade of NMDA receptors. Expression of Cx36 declined in PND28 compared
to PND14 in hippocampal neurons. Also, we found that the expression of Cx36 augmented in the rats'
hippocampal neurons in PND14 and PND28 following a blockade of NMDA receptors.

Conclusion:
These results suggest that the postnatal enhancement in glutamatergic synaptic activity is
associated with the loss of gap junctional connections and downregulation of Cx36 and Cx43 between
developing neurons and glial cells.
Associations Between microRNA-related Genetic Polymorphisms and Clinical
Response to Methotrexate in Chinese Rheumatoid Arthritis Patients
Authors: Wen Qin-Wen , Chen Chong-Jing , Zhang Han-Qing , Yu Hang , Zeng Zhen , Jin Ting , Wang Ting-Hui , Qin Wen , Huang Hua , Wu Xiu-Di , Cen Han 
Published: October 2022

Background:
Emerging evidence indicates that microRNA (miRNA)-related genetic polymorphisms
are strongly involved in the post-transcriptional regulation of the expression of pharmacokinetics and pharmacodynamics-related genes, therefore contributing to the genetic variability of drug response.

Objective:
To investigate the associations of miRNA-related genetic polymorphisms, including miRNA-5189
rs562929801, miRNA-595 rs4909237, SLCO1A2 rs4149009 and MTHFR rs3737966, and clinical response to
methotrexate in Chinese rheumatoid arthritis patients.

Methods:
One hundred patients treated with MTX for approximately 3 months were prospectively followed up
to evaluate the clinical response according to European League Against Rheumatism (EULAR) good and moderate response, disease activity score in 28 joint counts - erythrocyte sedimentation rate (DAS28-ESR) low disease activity (LDA) and remission (REM), change in DAS28-ESR (ΔDAS28-ESR) and ΔDAS28-ESR > 0.6.
Genetic polymorphisms were genotyped utilizing the HI-SNP technology.

Results:
Of the 100 patients with a mean age of 52.23 ± 12.71 years, 81 patients were female (81.00%). After
adjusting potential confounders, the major allele of miRNA-5189 rs562929801 was found to be significantly associated with EULAR response (A/A + A/G versus G/G, RR = 0.81, 95% CI = 0.67-0.99, P = 0.04) and
ΔDAS28-ESR > 0.6 under dominant model (A/A + A/G versus G/G, RR = 0.83, 95% CI = 0.71-0.98, P =
0.03). However, nonsignificant evidence was detected for the remaining three miRNA-related genetic polymorphisms in neither univariable analysis nor multivariable analysis.

Conclusion:
Our results indicated that miRNA-5189 rs562929801 was significantly associated with clinical response to MTX, and this association warrants further replication studies with larger sample sizes.
Integrating Network Pharmacology and Experimental Verification to
Investigate the Mechanisms of Shuangshi Tonglin Capsule to Treat Chronic
Prostatitis
Authors: Mao Qian , Zhang Xinyue , Wang Chuan , Liu Jiping , Wang Ziqiang , Wang Bin , Mao Peng , Wei Hao , Wang Baoan 
Published: October 2022

Background and Objective:
Chronic prostatitis (CP) is one of the most common
diseases in young and middle-aged men but lacks effective treatment. Shuangshi Tonglin Capsule
(SSTLC) is a clinical drug for the treatment of chronic prostatitis. However, the underlying molecular
mechanisms of SSTLC in treating CP are still unclear. In this study, we researched the underlying
mechanisms of SSTLC in treating chronic prostatitis.

Methods:
The ingredients of SSTLC were received from the TCMSP and BATMAN databases,
and the CP targets were collected based on GeneCards and OMIM. Then, the PPI network and the
“drug-ingredient-target” network map were constructed. GO and KEGG enrichment analyses by
using DAVID. Molecular docking was performed by using AutoDock 4.2 and PyMol. And using
animal experiments to verify the potential effect of SSTLC in CP.

Results:
SSTLC contained 10 herbs, 158 chemical ingredients and 277 targets, 2002, diseaserelated
targets were obtained. Network analysis outcomes indicated that VEGFA, TNF, MAPK1,
EGFR, and MAPK8 are the key targets of SSTLC in treating chronic prostatitis. Furthermore, molecular
docking revealed that quercetin, luteolin, and kaempferol exhibited a strong binding effect.
Animal experimental indicated that SSTLC can reduce the pathological damage to prostate tissue.
And, we found that high-dose SSTLC significantly reduced the level of TNF-α and downregulated
the expression of EGFR, p-p38 and p-ERK1/2 (P<0.05).

Conclusions:
This study determined the pharmacological effects of SSTLC and the potential mechanism
of action on SSTLC to treat CP, it provides a new idea for traditional Chinese medicine to
treat chronic prostatitis.
Chronic Cerebral Hypoperfusion-induced Dysregulations of Hyperpolarization-
activated Cyclic Nucleotide-gated, KCNQ and G Protein-coupled
Inwardly Rectifying Potassium Channels Correlated with Susceptibility
and Unsusceptibility to Anxiety Behaviors
Authors: Luo Pan , Liu Wei , Zhou Mei , Li Youwei , Lin Kuan 
Published: October 2022

Background:
Cerebrovascular lesions could induce affective disorders; however, the
depression- and anxiety-related symptoms caused by chronic cerebral hypoperfusion (CCH) and the
roles of different hyperpolarization-activated cyclic nucleotide-gated (HCN), KCNQ and G proteincoupled
inwardly rectifying potassium (GirK) channel subunits in these pathological processes have
been poorly elucidated so far.

Objective:
To investigate the behavioral change and the alteration of HCN, KCNQ, and GirK subunits
in amygdale rats suffering from CCH.

Methods:
Permanent bilateral occlusion of the common carotid arteries was used to induce CCH.
Anxiety and depression levels were assessed by the elevated plus maze test, sucrose preference test
and forced swimming test to classify rats as highly anxious or depressive ‘susceptibility’ vs. ‘unsusceptibility’.
The expression of brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor B
(TrKB), HCN1/2, KCNQ2/3, and GirK1/2/3 were quantified by Western blotting.

Results:
The main emotional change caused by 4 weeks of CCH is likely to be anxiety-like behavior
(50%), accompanied by a down-regulation of BDNF and TrKB expression in amygdale. The increase
of HCN1 and decrease of KCNQ3 expression in amygdale may be factors to blame for anxiety-
like symptom caused by CCH, and the increase of KCNQ2 and Girk1 expression in amygdale
may play a role in resilience to the anxiety induced by CCH.

Conclusion:
The different subunits of HCN, KCNQ and GirK channels in amygdale may contribute
to distinct response to aversive stimuli or stress induced by CCH that evokes divergent influences on
anxiety-like behavior in rats.
Apoptin Inhibits Glycolysis and Regulates Autophagy by Targeting
Pyruvate Kinase M2 (PKM2) in Lung Cancer A549 Cells
Authors: Song Gaojie , Shang Chao , Zhu Yilong , Xiu Zhiru , Li Yaru , Yang Xia , Ge Chenchen , Han Jicheng , Jin Ningyi , Li Yiquan , Li Xiao , Fang Jinbo 
Published: October 2022

Background:
Pyruvate kinase M2 (PKM2) is a key enzyme in aerobic glycolysis and plays
an important role in tumor energy metabolism and tumor growth. Ad-apoptin, a recombinant oncolytic
adenovirus, can stably express apoptin in tumor cells and selectively causes cell death in tumor cells.

Objective:
The relationship between the anti-tumor function of apoptin, including apoptosis and autophagy
activation, and the energy metabolism of tumor cells has not been clarified

Methods:
In this study, we used the A549 lung cancer cell line to analyze the mechanism of PKM2
involvement in apoptin-mediated cell death in tumor cells. PKM2 expression in lung cancer cells was
detected by Western blot and qRT-PCR. In the PKM2 knockdown and over-expression experiments,
A549 lung cancer cells were treated with Ad-apoptin, and cell viability was determined by the CCK-8
assay and crystal violet staining. Glycolysis was investigated using glucose consumption and lactate
production experiments. Moreover, the effects of Ad-apoptin on autophagy and apoptosis were analyzed
by immunofluorescence using the Annexin v-mCherry staining and by western blot for c-PARP,
p62, and LC3-II proteins. Immunoprecipitation analysis was used to investigate the interaction between
apoptin and PKM2. In addition, following PKM2 knockdown and overexpression, the expression
levels of p-AMPK, p-mTOR, p-ULK1, and p-4E-BP1 proteins in Ad-apoptin treated tumor cells
were analyzed by western blot to investigate the mechanism of apoptin effect on the energy metabolism
of tumor cells. The in vivo antitumor mechanism of apoptin was analyzed by xenograft tumor
inhibition experiment in nude mice and immunohistochemistry of tumors’ tissue.

Results:
As a result, apoptin could target PKM2, inhibit glycolysis and cell proliferation in A549 cells,
and promote autophagy and apoptosis in A549 cells by regulating the PKM2/AMPK/mTOR pathway.

Conclusion:
This study confirmed the necessary role of Ad-apoptin in the energy metabolism of A549
cells.