bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional and Transcriptome Analysis of Streptococcus pyogenes Virulence on Loss of Its Secreted Esterase
Authors: Zhang Xiaolan , Wang Yue , Zhu Hui , Zhong Zhaohua 
Published: July 2022

Esterases are broadly expressed in bacteria, but much remains unknown about their pathogenic effect. In previous studies, we focused on an esterase secreted by Streptococcus pyogenes (group A Streptococcus, GAS). Streptococcal secreted esterase (Sse) can hydrolyze the sn−2 ester bonds of platelet−activating factor (PAF), converting it to an inactive form that inhibits neutrophil chemotaxis to the infection sites. However, as a virulent protein, Sse probably participates in GAS pathogenesis far beyond chemotaxis inhibition. In this study, we generated the sse gene knockout strain (Δsse) from the parent strain MGAS5005 (hypervirulent M1T1 serotype) and compared the difference in phenotypes. Absence of Sse was related to weakened skin invasion in a murine infection model, and significantly reduced GAS epithelial adherence, invasion, and intracellular survival. Reduced virulence of the Δsse mutant strain was explored through transcriptome analysis, revealing a striking reduction in the abundance of invasive virulence factors including M protein, SIC, ScpA, and SclA. Besides the influence on the virulence, Sse also affected carbohydrate, amino acid, pyrimidine, and purine metabolism pathways. By elucidating Sse−mediated pathogenic process, the study will contribute to the development of new therapeutic agents that target bacterial esterases to control clinical GAS infections.
The West Kunlun Glacier Anomaly and Its Response to Climate Forcing during 2002–2020
Authors: Luo Jianwei , Ke Chang-Qing , Seehaus Thorsten 
Published: July 2022

Research into glacial mass change in West Kunlun (WK) has been sufficient, but most of the existing studies were based on geodetic methods, which are not suitable for specific health state analyses of each glacier. In this paper, we utilize Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) imagery, applying the continuity equation to obtain altitudinal specific mass balance (SMB) for 615 glaciers (>2 km2) during 2002–2011, 2011–2020, and 2002–2020 to research glacial health and its response to climatic forcing. The results show dissimilar glacier SMB patterns between 2002–2011 (0.10 ± 0.14 m w.e. a−1), 2011–2020 (–0.12 ± 0.14 m w.e. a−1) and 2002–2020 (−0.01 ± 0.07 m w.e. a−1). Additionally, the glacier equilibrium line altitude (ELA) in WK was 5788 m, 5744 m, and 5786 m, respectively, and the corresponding accumulation area ratios (AARs) were 0.59, 0.62, and 0.58, during 2002–2011, 2011–2020, and 2002–2020, respectively. Regarding glacier response, compared with the ordinary-least-square (OLS) model, the artificial neural network (ANN) model revealed a respectively less and more sensitive glacier SMB response to extreme negative and positive summer skin temperatures. In addition, the ANN model indicated that the glacier ELA was less sensitive when the integrated water vapor transport (IVT) change exceeded 0.7 kg m−1s−1. Moreover, compared with IVT (−121.57 m/kg m−1s−1), glacier ELA shifts were chiefly dominated by summer skin temperature (+154.66 m/℃) in the last two decades. From 2002–2011 and 2011–2020, glacier SMB was more susceptible to summer skin temperature (−0.38 m w.e./℃ and −0.16 m w.e./℃, respectively), while during 2002–2020, it was more influenced by IVT (0.45 m w.e./kg m−1s−1). In contrast with eastern WK, glaciers in western WK were healthier, although mitigation measures are still needed to safeguard glacier health and prevent possible natural hazards in this region. Finally, we believe that the inconsistent change between glacier SMB and ELAs from 2002–2020 was connected with ice rheology and that the combined effects of skin temperature and IVT can explain the WK glacier anomaly.
O Uso da impressora 3D no processo de ensino e aprendizagem.
Authors: Freitas Neto Antonio , Loubet Sara , Martinez Albuquerque Leonardo 
Published: July 2022

Sabe-se que no contexto atual da sociedade moderna, a tecnologia obteve consideráveis avanços na sociedade. Porém, é notório a existência de problemáticas persistentes nas práticas educacionais, as quais padecem com a falta da inclusão de tecnologias eficientes na educação, que por sua vez, tornariam as aulas mais lúdicas, capazes de capturar atenção dos discentes e consequentemente um maior aproveitamento e fixação do conteúdo ensinado em sala. Neste trabalho, apresentamos uma intervenção educacional a fim de amenizar as barreiras identificadas, utilizando para isto, a impressão 3D como forma de auxiliar no processo de ensino-aprendizagem, fabricando materiais didáticos, com o objetivo de despertar e aprimorar o interesse dos estudantes para as áreas de Ciências Exatas e da Terra, buscando assim realizar aulas mais práticas e experimentais. Os materiais didáticos produzidos neste trabalho foram aplicados em sala de aula, juntamente com questionários, os quais comprovam a eficiência da atividade, de forma que, para cerca de 90% dos discentes que participaram da atividade, houve uma melhor compreensão dos conteúdos trabalhados, tornando o processo de ensino-aprendizagem mais envolvente e significativo.
EDUCAÇÃO FÍSICA E GEOMETRIA: UMA EXPERIÊNCIA COM ALUNOS DO II PERÍODO DA EDUCAÇÃO INFANTIL
Authors: Dalvi Silvana , Tomaz Ana Elisa , Côco Lozorio Andressa 
Published: July 2022

O presente trabalho
tem por objetivo discutir as práticas pedagógicas na educação infantil através do circuito das formas geométricas. A atividade associou habilidades relacionadas a educação física e conceitos geométricos atendendo aos princípios da Base Nacional Comum Curricular, BNCC (2017) que consiste em desenvolver integralmente a criança por meio dos Campos de Experiências. A pesquisa é de abordagem qualitativa cujos dados foram extraídos na realidade dos alunos usando o diário de bordo, gravações e registro fotográfico. Os resultados revelam a intencionalidade da prática educativa proposta no circuito propiciando desenvolver nas crianças aspectos sociais, motores, afetivos e cognitivos. Ele favoreceu a integração entre o conhecimento e a formação humana privilegiando a ludicidade. Contudo, outras práticas podem ser articuladas a partir das interações e brincadeiras contribuindo para a qualidade e o pleno desenvolvimento das crianças na educação infantil.
The Prevalence of Low Vitamin D3 Levels in Patients with Lumbar Disc Herniation and Its Relationship with Different Patient Parameters
Authors: Qazi Rabail Akbar , Rehman Lal , Javeed Farrukh , Akbar Qazi Sana , Ahmed Tanweer , Abbas Asad 
Published: July 2022

Objective: To determine the prevalence of low vitamin D3 levels in patients having proven lumbar disc herniation and its relationship with different patient parameters.
Materials and Methods: This is a prospective study carried out on 100 patients at the Neurosurgery department, Jinnah postgraduate medical center from Feb 2018 to April 2019. Serum Vitamin D levels and other characteristics were assessed in patients with prolapsed lumbar intervertebral discs. Low levels of Vitamin D were defined as insufficiency (10 ng/ml – 30 ng/ml) and deficiency (< 10 ng/ml).
Results: Out of 100 patients in the study, only 21% had optimal serum vitamin D3 levels, and 79% had hypovitaminosis (57% insufficient and 22% deficiency). Low vitamin D levels were prevalent in all age groups, with deficiency more prevalent in the older age group (p-value = 0.004). The BMI (body mass index) of the patients showed a linear correlation with vitamin D levels.
Conclusion: Decreased vitamin D levels are prevalent in patients having herniated lumbar discs. The study showed that older-aged patients and high BMI exhibited severe vitamin D deficiency. As a result, recommendations are that preventive initiative for this specific hypovitaminosis target a broader population to intercept the occurrence of low vitamin D levels and the associated repercussions.
Clinical outcome and complication of anterior cranial Fossa meningiomas in tertiary care Hospital Peshawar
Authors: Hussain Arif , Khan Sajid , Hussain Ramzan , Ihsan Aleeha , Ali Mumtaz 
Published: July 2022

Objectives: We examined the surgical outcome and complications of anterior cranial Fossa meningiomas.
Materials and Methods: A prospective study was conducted at the Department of Neurosurgery Prime Teaching Hospital, Peshawar, KPK, Pakistan from June 2019 to June 2021. A total of 31 patients were selected through non-probability consecutive sampling. After clinical examination and radiological findings, the patients were diagnosed with anterior cranial Fossa meningiomas. Microsurgical Trans cranial approaches did. Clinical outcomes and complications were documented. Tumor anterior cranial fossa meningiomas. A low Karnofsky performance scale index was used to analyze the functional impairment of the patients.
Results: 9 (29.03%) were male and 22 (70.96%) patients were female. The mean age of presentation was 42 ± 5 years. Headache 25 (80.6%) and Visual loss were the main presenting symptoms 24 (77.5%), and Tuberculum sellae meningioma patients 08 (25.08%). The most common approach was Bifrontal craniotomy for OGM in 9 patients and two small OGM were operated on utilizing frontolateral craniotomy. The rest of the cases were operated through Pterional craniotomy. Tuberculamsalle meningiomas surgery results in a visual improvement in 6 patients (75%), Complete tumor resection was achieved in 22 (70.9%) patients and subtotal resection is achieved in 6 (19.3%) patients. In a few patients 03 (9.67%) only safe debulking and biopsy were taken. Postoperative hemorrhage was in 4 (12.9%) patients.
Conclusion: Transcranial approach for microsurgical resection of anterior cranial fossa meningiomas is an effective and feasible approach in terms of visual outcome and extent of resection.
Keywords: Anterior cranial Fossa Meningioma, Bifrontal Craniotomy, Front-lateral craniotomy.
Extrusion of the Peritoneal Catheter of Ventriculoperitoneal Shunt Through the Rectum
Authors: Utmanzai Sajid khan , Tariq Jamal  , Khalil Aafaq Ahmad Qarnain , Iqra muhammad ali  , Mumtaz ali  
Published: July 2022

A ventriculoperitoneal shunt is a common procedure for hydrocephalus. It is a life-saving procedure but is not risk-free. Some of the most common complications are shunt blockage and infection but they can also present with uncommon presentations. We report A child who presented with extrusion of a shunt catheter through the rectum. It was treated as an infected shunt. Externalization of the shunt was done through the abdominal site and the exposed shunt was removed through the rectum by gentle traction. Once CSF was clear a new shunt was placed on the opposite side.
Keywords: VP shunt, shunt infection, anal extrusion.
Key Message: Exposure to a shunt catheter through the rectum is an uncommon presentation. It should be treated as an infected shunt. Most of these cases do not cause peritonitis or meningitis. The exposed shunt catheter should be removed through the rectum by gentle traction.
Abbreviations: VP-Ventriculoperitoneal, CSF: Cerebrospinal fluid. ETV -Endoscopic Third Ventriculostomy.
Pediatric Posterior Fossa Brain Tumor Surgical Outcome
Authors: Mian Awais  , Akhtar Muner  , Laeeq-ur-Rehman  , Syed Misbah  , Rimla Ayesha  
Published: July 2022

Objective: The study analyzed the complications and satisfactory surgical outcomes of posterior fossa brain tumor surgery in Lahore Medical City Lahore.
Materials and Methods: A prospective study was conducted and included 40 children who were diagnosed with posterior fossa brain tumors by history, physical examination, and later radiologically were admitted and operated on at the Pediatric Neurosurgery Department in Lahore medical City Lahore between the period of March 2021 and March 2022.
Results: There were 26 (65%) male and 14 (35% females) individuals among the 40 patients. The average age was 12.5 years. This study found that great surgical outcomes were observed in 10 instances (25 percent), good outcomes in 20 cases (50%), and bad outcomes in 10 cases (25%). The most frequent clinical manifestations were headache (38%), vomiting (30%), ataxia (10%), blurred vision (10%), and cranial nerve palsy (12.5%). The best prognosis is shown in children with Pilocytic astrocytoma, followed by ependymoma, while the poorest outcome is seen in children with medulloblastoma.
Conclusion: Pediatric neurosurgeons continue to face particular difficulty in the surgical treatment of posterior fossa brain tumors. Our study compares the outcomes, complications, and surgical outcomes to prior clinical investigations.
Efficacy of Posterior and Posterolateral Approach for Decompression and Fusion for Thoracolumbar Tuberculosis
Authors: Muhammad Adeel Razzaque  , Waqas Noor Chughtai  , Sumera Nighat  , Muhammad Tahir  , Shakeel Ahmad  , Moazma Nawaz  , Aaiza Rehman  
Published: July 2022

Objective: To study the efficacy of the posterior and posterolateral approach in thoracolumbar tuberculosis.
Material and Methods: 60 patients with thoracolumbar TB spine were enrolled in the study from 2015 to 2021. These patients had single-level disease with low back pain and neurological compromise in lower limbs. The diagnosis was made on an MRI of the spine and elevated ESR levels. All patients were started with antituberculous treatment. The pain was measured on the VAS score, and neurological status was assessed on the ASIA score. Kyphosis angle was calculated on a lateral x-ray of the spine. All patients were operated on by posterior and posterolateral approaches with decompression and fusion. At follow-up, fusion was assessed on every visit by x-ray along with neurological status and pain score.
Results: The mean age of patients was 45.8 years (25 to 66 years). 59.5% patients achieved radiological fusion on follow-up x-rays. There was a significant improvement in VAS score preoperatively mean and SD6.38 ± 1.24to postoperatively 4.45 ± 1.09. The mean and SD of kyphosis in patients preoperatively was 22.3 ± 3.06 to post-operative 22.3 ± 3.06 with a p-value < 0.05 which shows significant improvement.
Conclusion: Posterior and posterolateral decompression and fusion of thoracolumbar tuberculosis is a good surgical approach in respect of neurological outcome, correction of kyphosis, and pain improvement.
<p>Pembentukan Citra Positif Melalui Aspek Gramatika dalam Wacana Alat Peraga Kampanye (<em>Forming of Positive Image through Grammatical Aspects in the Discourse of Campaign Props</em>)</p>
Authors: Nugroho Endro , Sudono Agus 
Published: July 2022

Dalam setiap pemilihan umum, calon legislatif menggunakan pelbagai cara untuk mengambil hati rakyat. Hal tersebut tampak pada masa kampanye. Calon wakil rakyat memberikan janji-janji manis pada masyarakat pemilih. Ungkapan yang bombastis sering dijumpai di berbagai alat peraga kampanye, seperti baliho, kain rentang, atau poster-poster di jalan. Penelitian ini mengungkap keterkaitan aspek gramatika dengan aspek-aspek di luar kebahasaan, seperti aspek sosial dan politik yang terdapat di dalam wacana alat peraga kampanye Pemilu 2019. Metode yang digunakan dalam penelitian ini adalah analisis wacana kritis. Analisis wacana kritis tidak hanya mengkaji bahasa dari aspek bahasa saja, tetapi juga mengaitkannya dengan konteks sosial dan budaya. Kajian terhadap aspek gramatika meliputi kajian terhadap ketransitifan, pilihan kalimat aktif-pasif, pilihan kalimat positif-negatif, dan modalitas relasional. Aspek-aspek tersebut dimanfaatkan untuk membentuk citra positif (personal branding) caleg yang pada akhirnya bertujuan untuk meningkatkan elektabilitas mereka. In every general election, legislative candidates use various ways to win the hearts of the people. This can be seen during the campaign. Candidates for people's representatives make sweet promises to the voting public. Bombastic expressions are often found, one of which is found in campaign props, such as billboards or posters on the street. This study reveals the relationship between grammatical aspects and aspects outside of language, such as social and political aspects contained in the discourse of props for the 2019 election campaign. The method used in this study is a critical discourse analysis. The critical discourse analysis does not only study language from the language aspect, but also relates it to social and cultural contexts. The study of grammatical aspects includes a study of transitivity, active-passive choice of sentences, choice of positive-negative sentences, and relational modalities. These aspects are used to form a positive image (personal branding) of candidates which ultimately aims to increase their electability.
HEALTH EDUCATION ON THE IMPORTANCE OF WASHING HANDS WITH SOAP (CTPS) AT SDN 10 DUNGALIYO
Authors: Prasetya Ekawaty , Jusuf Herlina , Ahmad Zulfikar 
Published: July 2022

Perilaku Hidup Bersih dan Sehat (PHBS) merupakan salah satu cara masyarakat untuk dapat menjaga kualitas kesehatannya. Program PHBS terdapat beberapa indikator yang dijadikan acuan dalam pelaksanaan PHBS. salah satunya adalah cuci tangan pakai sabun (CTPS). Perilaku CTPS yang baik dapat mencegah kejadian diare dan ISPA yang rentan dialami oleh anak-anak khususnyaanak usia sekolah. Sehingga penting untuk mengajarkan anak sejak dini mengenai CTPS yang baik dan benar. Namun pembelajaran mengenai kesehatan di sekolah sering menggunakan metode dan media yang tidak tepat untuk anak-anak khususnya anak usia sekolah. Kebaruan penelitian ini adalah untuk memberikan edukasi terkait pentingnya menerapkan cuci tangan pakai sabun. Tujuan kegiatan adalah untuk memberikan pemahaman pentingnya pentingnya health edukasi perilaku cuci tangan pakai sabun (CPTS) di SD Negeri 10 Dungaliyo. Metode kegiatan melalui penyuluhan dan pendidikan kesehatan tentang CTPS baik dan benar di sekolah. Hasil: berdasarkan hasil dari kegiatan ini dapat dilihat nanti pada saat PBL 3 setelah 6 bulan dari sekarang, apakah mengalami peningkatan setelah diberikan penyuluhan dan edukasi tentang CTPS yang baik dan benar. Kesimpulan terjadi peningkatan pengetahuan siswa tentang pentingnya CPTS setelah pendidikan kesehatan. Kata kunci: Health Edukasi; Cuci Tangan Pakai Sabun (CPTS) AbstractClean and Healthy Living Behavior (PHBS) is one way for the community to be able to maintain the quality of their health. The PHBS program has several indicators that are used as a reference in the implementation of PHBS. One of them is washing hands with soap (CTPS). Good CTPS behavior can prevent the incidence of diarrhea and ARI which are vulnerable to being experienced by children, especially school-age children. So it is important to teach children from an early age about good and correct CTPS. However, learning about health in schools often uses inappropriate methods and media for children, especially school-age children. The novelty of this research is to provide education regarding the importance of applying hand washing with soap. The purpose of the activity is to provide an understanding of the importance of health education on handwashing with soap (CPTS) at SD Negeri 10 Dungaliyo. The method of activity is through counseling and health education about good and correct CTPS in schools. Results: based on the results of this activity, it can be seen later during PBL 3 after 6 months from now, whether it has increased after being given counseling and education about good and correct CTPS. The conclusion is that there is an increase in students' knowledge about the importance of CPTS after health education.Keywords: Health Education; Hand Washing With Soap (CPTS).
Covid-19 and Domestic Violence in Kashmir: Gender Voices.
Authors:   Research Scholar, Department of Political Science, University of Kashmir, Srinagar, Mugloo Anayat Ul Lah ,   Jammu and Kashmir., Khan Imran Ahmad ,   Research Scholar, Department of Political Science, University of Kashmir, Srinagar; Jammu and Kashmir., Mir Tawseef Ahmad ,   Research Scholar, Centre of Central Asian Studies, University of Kashmir, Srinagar; Jammu and Kashmir.
Published: July 2022

Domestic violence has become a global pandemic long before the COVID-19 outbreak. It is claimed that due to covid induced lockdown a continual rise is spotted in the numbers of sick people; growing unemployment, increased anxiety and financial stress, scarcity of community resources etc. Resultantly, these factors are believed to have set the stage for an exacerbated hype in domestic violence cases. Experts have characterized the “invisible pandemic” of domestic violence as a “ticking time bomb” or a “perfect storm”. The connection between physical insecurity and economic insecurity is claimed to be tangible for people who might be less attuned to domestic violence. There is now a unique opportunity to shed light on the economic dimensions of domestic and gender-based violence. Indeed, a thorough examination and deep introspection of the impact of the novel diseases need to be mapped out. However, it needs to be noted down that the present study is limited to the region of Kashmir. At the same time rising domestic violence against women in Kashmir Valley during the pandemic lockdown of COVID-19 is matter of concern and need to be highlighted and simultaneously efforts should be made (on part of Government, NGOs or others) to contain such violence. A detailed study of such pandemic accelerated gender-based violence seems a must. A study which could possibly map out the role of different factors ranging from age, education, job, etc. to others on the acceleration or maximization of domestic violence in Kashmir amidst of pandemic. After taking all those pros and cons into consideration, the present study set itself to examine the relationship between covid-19 and domestic violence in Kashmir. All the ten districts of the Kashmir region were included in study, and among each district a maximum of 21 participants were selected. To reach the desired end and draw the results, the present paper has undertaken an online questionnaire.
Dynamical Star-forming History of Per OB2
Authors: Kounkel Marina , Deng TingYan , Stassun Keivan G. 
Published: July 2022

Abstract
We analyze the internal dynamics of young stars toward Perseus using Gaia EDR3 data, including Per OB2 and California Cloud. Interpreting the current dynamics, we speculate that Per OB2 may have formed from two separate clouds that have begun forming stars in close proximity to each other. IC 348 is caught in the middle between the two of them, inheriting kinematics of both, and it stands out as a possible site of cloud–cloud interaction. We also consider the possibility of a past supernova in Per OB2—while one has likely occurred, it does not appear to have caused any obvious triggered star formation, but it has created a shock that has swept the molecular gas away from IC 348. Finally, we examine a recently proposed shell between Taurus and Perseus. While its origin is unknown, we find no support for an expanding bubble in stellar kinematics, nor can we identify a likely progenitor for a supernova that may have caused it, disfavoring this scenario in the formation of this apparent shell.