bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ferulic Acid Induces Bone Marrow Mesenchymal Stem Cells to Alleviate the Activation of Hepatic Stellate Cells and Liver Fibrosis via Cytoskeletal Rearrangement Inhibition and Mir-19b-3p Transfer
Authors: Zhang Rui , Li Wenhang , Jiang Xiandan , Cui Xinyi , You Hongjie , Tang Zuoqing , Liu Wenlan , Wang Jiabo 
Published: January 2022

Abstract
Background: Bone marrow mesenchymal stem cells (BMSCs) are effective for treating fibrotic liver. BMSCs contain a variety of proteins and RNAs, which have functions similar to their derived cells, but the specific mechanism is unclear. In a recent study, ferulic acid (FA) was highly effective in treating liver fibrosis. Therefore, we combined BMSCs and FA to treat CCl4-induced fibrosis models. Methods: First, we used BMSCs and FA to treat CCl4-induced fibrosis models and observed their therapeutic effect, investigated the specific mechanism of this combination therapy in liver fibrosis. Second, we created a BMSC/hepatic stellate cell (HSC) co-culture system and used FA to treat activated HSCs. We next used cytochalasin D and angiotensin II to investigate whether BMSCs and FA inactivate HSCs through cytoskeletal rearrangement. MiR-19b-3p was enriched in BMSCs and targeted TGF-β receptor II (TGF-βR2). We transfected miR-19b-3p into HSCs and BMSCs separately and detected whether BMSCs transferred miR-19b-3p to HSCs or inactivated HSCs. Results: We used BMSCs and FA to treat CCl4-induced fibrosis models and found that the combination therapy had better effects than FA or BMSCs alone. The expression of the profibrotic markers α-SMA and COL1-A1 was significantly decreased in HSCs co-cultured with BMSCs and FA treatment. Cytoskeletal rearrangement in HSCs was inhibited, and RhoA/ROCK pathway gene expression was decreased. With angiotensin II treatment, COL1-A1 and a-SMA expression increased, while with cytochalasin D treatment, profibrotic gene expression decreased in HSCs. COL1-A1, α-SMA and RhoA/ROCK pathway genes were decreased in activated HSCs treated with a miR-19b-3p mimic, indicating that miR-19b-3p inactivated HSCs by suppressing RhoA/ROCK signalling. In contrast, profibrotic genes were significantly decreased in BMSCs treated with the miR-19b-3p mimic or a miR-19b-3p inhibitor and FA compared with BMSCs treated with the miR-19b-3p mimic alone.Conclusion: BMSCs attenuated HSC activation and liver fibrosis by inhibiting cytoskeletal rearrangement and delivering miR-19b-3p to activated HSCs, inactivating RhoA/ROCK signaling. FA-based combination therapy showed better inhibitory effects on HSC activation, suggesting that BMSCs and their miRNAs combined with FA are novel antifibrotic therapeutics for treating chronic liver disease.
Induction of Donor-Specific Immune Hypo-Responsiveness Across Major Histocompatibility Complex Barriers in Murine Models of Solid Organ Transplant: Repurposing Extracorporeal Photochemotherapy
Authors: Schneiderman Jennifer , Qiu Longhui , Yeap Xin Yi , Kang Xin , Zheng Feibo , Ye Junsheng , Xie Yan , Wang Jiao-Jing , Sambandam Yuvaraj , Mathew James , Li Lin , Leventhal Joseph , Edelson Richard , Zhang Zheng Jenny 
Published: January 2022

Abstract
Recipients of solid organ transplantation (SOT) rely on life-long immunosuppression (IS), which is associated with significant side effects. Extracorporeal photochemotherapy (ECP) is a safe, existing cellular therapy used to treat transplant rejection by modulating the recipient’s own blood cells. We sought to induce donor-specific hypo-responsiveness of SOT recipients by infusing ECP-treated donor leukocytes prior to transplant. To this end, we utilized major histocompatibility complex (MHC) mismatched rodent models of allogeneic cardiac, liver, and kidney transplantation to test this novel strategy. Leukocytes isolated from donor-matched spleens for ECP treatment (ECP-DL) were infused into transplant recipients seven days prior to SOT. Pre-transplant infusion of ECP-DL without additional IS was associated with prolonged graft survival in all models. This innovative approach promoted the production of tolerogenic dendritic cells and regulatory T-cells with subsequent inhibition of T-cell priming and differentiation, along with a significant reduction of donor-specific T-cells in the spleen and grafts of treated animals. This new application of donor-type ECP-treated leukocytes provides insight into the mechanisms behind ECP-induced immunoregulation and holds significant promise in the prevention of graft rejection and reduction in need of global immune suppressive therapy in patients following SOT.
XRE Family Transcriptional Regulator XtrSs Modulates Streptococcus Suis Fitness of Hydrogen Peroxide Stress
Authors: Zhang Yumin , Liang Song , Pan Zihao , Yu Yong , Yao Huochun , Liu Yongjie , Liu Guangjin 
Published: January 2022

Abstract
Streptococcus suis is an important emerging zoonosis that causes economic losses in the pig industry and severe threats to public health. Transcriptional regulators play essential roles in bacterial adaptation to host environments. In this study, we identified a novel XRE family transcriptional regulator in S. suis CZ130302, XtrSs, involved in the bacterial fitness to hydrogen peroxide stress. Based on electrophoretic mobility shift and β-galactosidase activity assays, we found that XtrSs autoregulated its own transcription and repressed the expression of its downstream gene psePs, a surface protein with unknown function in S. suis, by binding to a palindromic sequence from the promoter region. Furthermore, we proved that the deletion of the psePs gene attenuated bacterial antioxidant response. Phylogenetic analysis revealed that XtrSs and PsePs naturally co-existed as a combination in most S. suis genomes. Collectively, we demonstrated the binding characteristics of XtrSs in S. suis and provided a new insight that XtrSs played a critical role in modulating psePs to the hydrogen peroxide resistance of S. suis.
The Study of CFRP Variable Feed Drilling Method Based on Sinusoidal Curve
Authors: Wang Shuaipu , Liang Jie 
Published: January 2022

Abstract
In order to reduce the delamination damage of carbon fiber reinforced polymer (CFRP) drilling and improve the drilling quality. A variable feed drilling method based on sinusoidal curve was proposed, that is, when the drill at a distance of 1mm from the hole exit, the feed rate of the drill would be reduced according to the rule of sinusoidal curve. The method is compared with the traditional feed drilling by experiment, and the influence of the variable feed drilling on thrust force, delamination factor, surface quality, surface roughness of hole wall and exit hole wall morphology are analyzed. The results show that compared with constant-feed drilling, the variable-feed drilling based on sinusoidal curve reduces the thrust force near the hole exit, and greatly improves the delamination factor, surface quality and hole wall morphology at the exit. But the influence on the hole wall roughness is not particularly obvious.
Neural Substrates Associated With Fatigue Symptom In Fibromyalgia
Authors: Liu Hung-Yu , Lee Pei-Lin , Chou Kun-Hsien , Wang Yen-Feng , Chen Shih-Pin , Lai Kuan-Lin , Lin Ching-Po , Wang Shuu-Jiun , Chen Wei-Ta 
Published: January 2022

Abstract
Many patients with fibromyalgia (FM) experience fatigue, but the associated biological mechanisms have not been delineated. We aimed to investigate the neural signatures associated with fatigue severity in patients with FM using MRI. We consecutively recruited 138 patients with FM and collected their clinical profiles and brain-MRI data. We categorized the patients into 3 groups based on their fatigue severity. Using voxel-based morphometry analysis and trend analysis, we first identified neural structures showing volumetric changes associated with fatigue severity, and further explored their seed-to-voxel structural covariance networks (SCNs). Results showed decreased bilateral thalamic volumes were associated with higher severity of fatigue. There was a more widespread distribution of the thalamic SCNs to the frontal, parietal, subcortical, and limbic regions in patients with higher fatigue severity. In addition, increased right inferior temporal cortex volumes were associated with higher severity of fatigue. The right inferior temporal seed showed more SCNs distributions over the temporal cortex and a higher strength of SCNs to the bilateral occipital cortex in patients with higher fatigue severity. The thalamus and the right inferior temporal cortex as well as their altered interactions with cortical and subcortical regions comprise the neural signatures of fatigue in FM.
Structural and computational insights into the SARS-CoV-2 Omicron RBD-ACE2 interaction
Authors: Wang Xinquan , Lan Jun , He Xinheng , Ren Yifei , Wang Ziyi , Zhou Huan , Fan Shilong , Zhu Chenyou , Liu Dongsheng , Shao Bin , Liu Tie-Yan , Wang Qisheng , Zhang Linqi , Ge Jiwan , Wang Tong 
Published: January 2022

Abstract
Since SARS-CoV-2 Omicron variant (B.1.1.529) was reported in November 2021, it has quickly spread to many countries and outcompeted the globally dominant Delta variant in several countries. The Omicron variant contains the largest number of mutations to date, with 32 mutations located at spike (S) glycoprotein, which raised great concern for its enhanced viral fitness and immune escape[1-4]. In this study, we reported the crystal structure of the receptor binding domain (RBD) of Omicron variant S glycoprotein bound to human ACE2 at a resolution of 2.6 Å. Structural comparison, molecular dynamics simulation and binding free energy calculation collectively identified four key mutations (S477N, G496S, Q498R and N501Y) for the enhanced binding of ACE2 by the Omicron RBD compared to the WT RBD. Representative states of the WT and Omicron RBD-ACE2 systems were identified by Markov State Model, which provides a dynamic explanation for the enhanced binding of Omicron RBD. The effects of the mutations in the RBD for antibody recognition were analyzed, especially for the S371L/S373P/S375F substitutions significantly changing the local conformation of the residing loop to deactivate several class IV neutralizing antibodies.
Sportsman’s Mental State Evaluation and Early Warning Method Using Decision Tree
Authors: Kumar Nitin 
Published: January 2022

Abstract
Aiming at the intelligent needs of psychological state assessment of university students, the text information-based psychological problem identification approach is investigated in the paper. This approach uses the text of student forums within universities as the database and introduces the convolutional neural network (CNN) model in deep learning, which contains a convolutional layer, a pooling layer, and a fully connected layer. After the convolution is completed, the convolution result is de-linearized by the activation function, and then pooling is performed to improve the fitting ability of the network for nonlinearities. For data processing, behavioral features attribute features, content features, and social relationship features are extracted from text information as the input of the CNN by using the decision tree. The psychological lexicon of expertise (LIWC) is used to enhance the efficiency of text word frequency statistics when performing text content extraction. To evaluate the performance of the proposed method, simulations are performed in the open dataset of CLPsyh2017 ReachOut Forum, and the FastText method is used as a comparison. The results show that the CNN model achieves an accuracy of 0.71 in the full-sample domain, which is significantly higher than that of the FastText model at 0.64. In the early warning evaluation of mental states, the CNN performance is better than that of FastText.
Kendall Transformation: A Robust Representation of Continuous Data for Information Theory
Authors: Kursa Miron Bartosz 
Published: January 2022

Abstract
Kendall transformation is a conversion of an ordered feature into a vector of pairwise order relations between individual values. This way, it preserves ranking of observations and represents it in a categorical form.
Such transformation allows for generalisation of methods requiring strictly categorical input, especially in the limit of small number of observations, when discretisation becomes problematic.In particular, many approaches of information theory can be directly applied to Kendall-transformed continuous data without relying on differential entropy or any additional parameters. Moreover, by filtering information to this contained in ranking, Kendall transformation leads to a better robustness at a reasonable cost of dropping sophisticated interactions which are anyhow unlikely to be correctly estimated.
In bivariate analysis, Kendall transformation can be related to popular non-parametric methods, showing the soundness of the approach.The paper also demonstrates its efficiency in multivariate problems, as well as provides an example analysis of a real-world data.
Experimental Evidence for Manure-Borne Bacteria Invasion in Soil During a Coalescent Event: Influence of the Antibiotic Sulfamethazine
Authors: Billet Loren , Pesce Stéphane , Martin-Laurent Fabrice , Devers-Lamrani Marion 
Published: January 2022

Abstract
The fertilization of agricultural soil by organic amendment that may contain antibiotics, like manure, can transfer bacterial pathogens and antibiotic-resistant bacteria to soil communities. However, the invasion by manure-borne bacteria in amended soil remains poorly understood, being hardly observed. Here, we assessed the invasions of manure-borne bacteria during a coalescence event between manure and soil, in different soils and in the presence or absence of antibiotics. To this end, microcosms of four different soils were amended or not with manure at an agronomical dose and/or exposed or not to the antibiotic sulfamethazine (SMZ). After one month of incubation, the diversity, structure and composition of bacterial communities of the soils were assessed by 16S rDNA sequencing. The invasion of manure-borne bacteria was still perceptible one month after the soil amendment. The results obtained with the soil already amended in situ with manure six months prior to the experiment suggest that some of the bacterial invaders were established in the community over the long term. Even if differences were observed between soils, the invasion was mainly attributable to some of the most abundant OTUs of manure (mainly Firmicutes). SMZ exposure had a limited influence on soil microorganisms. It was significant in only one soil, where it enhanced the invasion potential of some manure-borne invaders.
Arsenic Induced Gallbladder Cancer Risk in Eastern Part of Indo-Gangetic Plains
Authors: Kumar Arun , Ali Mohammad , Raj Vivek , Kumari Arti , Rachhamala Mahesh , Niyogi Som , Kumar Dhruv , Sharma Ashok , Saxena Amit , Panjawani Ghanish , Jain Preeti , Vidyarthi Ajay , Kumar Mukesh , Niraj Pintoo Kumar , Rahman Md. Samiur , Bishwapriya Akhouri , Kumar Ranjit , Sakamoto Maiko , Kumar Santosh , Singh Manisha , Ghosh Ashok Kumar 
Published: January 2022

Abstract
BackgroundIn recent times Gallbladder cancer (GBC) incidences increased many folds in India. Majority of GBC cases are being reported from arsenic hotspots identified in Bihar. MethodsIn this prospective study volunteers were selected who underwent surgery in our cancer institute. There were 11 control benign gallbladder cases and 28 confirmed gallbladder cancer cases. Their biological samples such as blood, gallbladder tissue, gallbladder stone, bile and hair samples were collected for arsenic estimation. Moreover, n=512 gallbladder cancer patients blood samples were evaluated for the presence of arsenic to understand exposure level in the population. ResultsA significantly high arsenic concentration (p<0.05) was detected in the blood samples, gallbladder tissue, gallstones, bile and hair samples in comparison to the control group. Moreover, n=512 blood samples of GBC patients had significantly very high arsenic concentration. ConclusionsThe study shows very high arsenic concentration observed in the blood, gallbladder tissue, gallbladder stone, bile and hair samples in GBC cases in comparison to the benign control cases indicates the correlation between chronic arsenic exposure and gallbladder cancer incidences in eastern Indo-Gangetic plains region. The study also makes an attempt to establish the likely correlation/association between arsenic exposure and gallbladder cancer disease.
Visualising Iron Gall Ink Underdrawings In 16th Century Paintings In-Situ By Micro-XRF Scanning (MA-XRF) And LED-Excited IRR (LEDE-IRR)
Authors: Gerken Mareike , Sander Jochen , Krekel Christoph 
Published: January 2022

Abstract
Until today, iron gall ink is classified as an exceptional underdrawing material for paintings. A certain identification is always based on invasive analysis. This article presents a new non-destructive analysis approach using micro-X-ray fluorescence scanning (MA-XRF), LED-excited IRR (LEDE-IRR) using a narrow wavelength-range of infrared radiation (IR) and stereomicroscopy for visualising and identifying iron gall ink underdrawings. To assess possibilities and limits of this non-invasive approach, results were compared to invasive examinations on cross-sections using scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX). The approach is tested on panel paintings of Hans Holbein the Elder and Giovanni Battista Cima da Conegliano. The holistic setup could successfully visualise underdrawing lines made with iron gall inks, which formerly remained invisible by means of conventional IRR. For the first time, a direct access to a formerly invisible type of underdrawing is created, allowing to harness the whole iron gall ink underdrawing for interdisciplinary studies.
Associationj of TSHR Gene Single Neucleotide Intronic Polymorphism with the Risk of Hypothyroid and Hyperthyroid Disorders in Yazd Province.
Authors: Naghibi Fahime , Miresmaili Seyed Mohsen , Javid Amaneh 
Published: January 2022

Abstract
The present study was carried, for the first time, out to evaluate the association of rs2268458 polymorphism, biochemical and environmental factors on hypothyroid and hyperthyroid disorders in thyroid patients and healthy individuals in Yazd province, Iran. In this study, blood samples were collected from a total of 100 cases, including 60 hypothyroid, 20 hyperthyroidism individual cases and 20 normal individuals. DNA was extracted from blood samples and the rs2268458 single neucleotide intronic polymorphism was evaluated using RFLP-PCR. The results have shown that 59 cases were homozygote (TT), 40 cases heterozygote (TC) with one homozygote (CC) case, as follows; A total of 25 (TT) homozygote cases were observed to be hypothyroid females, 20 (TC) heterozygote cases of hypothyroid females, 7 (TT) homozygote male hypothyroid cases and 7 (TC) heterozygote male hypothyroid cases and 1 (CC) homozygote male hypothyroid patient. While, 7 (TT) homozygote hyperthyroid female cases, 8 (TC) heterozygote hyperthyroid female cases,were also observed. According to our study, heterozygote cases (TC) showed less severe symptoms, while homozygote cases (TT) showed no serious symptoms and the (CC) homozygote case (CC) showed severe thyroid abnormality symptoms. So, it can be concluded that the TSHR-related rs2268458 polymorphism is associated with hypothyroidism and hyperthyroidism in the male and female polulations of Yazd Province, Iran and C allele can be a risk factor for some physio-biochemical and hormonal imbalance in the thyroid disorder patients.
Design of a Fully Integrated VHF CP-PLL Frequency Synthesizer with an All-Digital Defect-Oriented Built-In Self-Test.
Authors: Kommey Benjamin , Addo Ernest , Yankey Jepthah , Agbemenu Andrew , Tchao Eric , Akowuah Bright 
Published: January 2022

Abstract
This paper presents the design of an on-chip charge pump phase-locked loop (CP-PLL) with a fully digital defect oriented built-in self-test (BIST) for very-high frequency (VHF) applications. The frequency synthesizer has a 40 to 100 MHz tuning range and uses a ring voltage-controlled oscillator for frequency synthesis. The PLL exhibits a phase noise of -132 dBc/Hz at 1 MHz and consumes 1.8 mW on a 3 V supply. The BIST implementation uses fewer external input or output, is capable of efficient fault diagnosis, and is compact, posing a low area overhead. The integrated circuit design was realized in the AMI 0.6µ complementary metal oxide-semiconductor process.
Modelling Outpatients Cost of Wound Dressing in Nigeria: A Prospective Study
Authors: Ogundeji Kolawole Damilare 
Published: January 2022

Abstract
Background: Estimating the direct cost of wound dressing poses a challenge to patients, nurse managers, hospital administrators, health maintenance organisations and other policy makers. This study therefore model the weekly cost of wound dressing in South West NigeriaMethods: A descriptive cross sectional research design was utilized to assess the cost of wound dressing among outpatients’ clinics attendees. An inventory of direct cost of wound dressing per week consisting of cost of materials, lotion and consumables were recorded. The data collection was for period of three months in three selected Teaching Hospitals South West Nigeria. Then modelling weekly cost of wound types was done by regression analysis. The effect of various independent variables such as age, occupation, family size, monthly income, aetiology, diagnosis, wound type, comorbidities, frequency of wound dressing and health insurance coverage on cost of wound dressing per week was considered. Ethical approval was obtained from each of the hospital and Covid-19 precautions were observed.1 USD equalled ₦570Results: The estimated cost of wound dressing per week with no contribution from other variables was found to be ₦36,922- Open wound, ₦6011-Leg ulcer, ₦3768- Cancer wound, ₦2785- Diabetic Foot Ulcer, ₦610-Surgical wound. Only frequency of wound dressing was found to contribute to weekly cost of leg ulcers (P value= 0.003)Conclusions: The estimated cost of various type of wound provides yardstick for determining the weekly cost of wound dressing in Nigeria. Also, frequency of wound dressing is a major determinant of the outpatient cost of dressing. Therefore, it is imperative for nurses to render high quality wound care to match up with the huge financial commitment from patients and families