bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the Farm Animals Immune to Pathogens of Zoonotic Infectious Diseases in the Republic of Guinea
Authors: Naidenova E. V. , Kartashov M. Yu. , Shevtsova A. P. , Shipovalov A. V. , Kabanov A. S. , Boldyrev N. D. , Krivosheina E. I. , Diallo M. G. , Nassour A. A. , Bah M. B. , Nourdine I. , Keyra M. , Kaba L. , Camara S. , Diallo M. T. , Boumbaly S. , Sidime Y. , Kutyrev V. V. 
Published: July 2022

The most common anthropozoonoses on the African continent are coxiellosis and Rift Valley fever. It is known that detection of specific IgG antibodies in the blood sera of farm animals is one of the indicators of the pathogen circulation in a certain territory. The aim of the work was to identify specific IgG antibodies in the blood sera of farm animals collected on the territory of the Republic of Guinea to pathogens of zoonotic infectious diseases: coxiellosis, brucellosis, glanders, CCHF, West Nile and Rift Valley fevers, using enzyme immunoassay (ELISA). Materials and methods. A panel of 970 samples of blood sera from farm animals inhabiting all landscape-geographical zones of Guinea was compiled for the work. Identification of specific antibodies was carried out using enzyme immunoassay with preparations recommended for veterinary studies. Results and discussion. Specific antibodies to zoonoses were detected in 700 out of 1074 samples (65.2 % of the total), including: to Coxiella burnetii – in 172 (16.0 %); to Brucella spp. – in 212 (19.7 %); viruses of Rift Valley fever – 85 (7.9 %); CCHF – in 139 (12.9 %) and West Nile fever – in 92 (8.6 %). Antibodies to Burkholderia mallei were not found in the tested material. Positive samples were registered in all landscape-geographical zones. Thus, an urgent task is to continue studying the circulation of pathogens of zoonoses and anthropozoonoses in the territory of the Republic of Guinea and to organize regular monitoring over the spread of zoonotic infectious diseases in collaboration with veterinary services, which will allow timely forecasting and coordinating prophylactic (anti-epidemic) measures to prevent cases of diseases.
Infectious Diseases in the Americas Region that Are Relevant to the Global Surveillance and Control
Authors: Udovichenko S. K. , Nikitin D. N. , Boroday N. V. , Ivanova A. V. , Putintseva E. V. , Viktorov D. V. , Toporkov A. V. , Kostyleva A. A. 
Published: July 2022

The aim of the work was information-analytical assessment of the epidemiological situation on infectious diseases that are potentially or truly dangerous in terms of occurrence of emergencies of sanitary-epidemiological nature in the Region of Americas.Materials and methods. The study was based on the official reports of the WHO, the Pan American Health Organization, the Centers for Disease Control and Prevention, the national Ministries of Health, data from the ProMED information portal, the Global Network for the Epidemiology of Infectious Diseases, and published scientific papers.Results and discussion. By the model of the Americas, regional epidemiological features have been established, including the endemicity (enzooticity) of territories according to the most relevant nosological forms and the intensity of the epidemic process manifestations. It is shown that the main epidemiological risks in the countries of Central, South America and the Caribbean are associated with dengue, Zika, Chikungunya fevers characterized by a wide territorial dissemination and the ability to cause large-scale epidemic outbreaks, in the countries of North America – West Nile fever. Other infections of international concern include: cholera, that twice caused epidemics of imported origin during the seventh pandemic, which changed the structure of world morbidity; plague, manifested in an annual incidence, including with a complication by the pneumonic form, which determines an increased potential danger of anthropogenic spread; malaria, demonstrating an upward trend in morbidity and the number of intra-continental imported cases; yellow fever, characterized by the activation of natural foci and the expansion of the territories of potential pathogen transmission. The data obtained can serve as a basis for assessing the risks of infectious disease introduction from the American Region into safe territories, improving epidemiological forecasting and validity in making managerial decisions when conducting sanitary and anti-epidemic (preventive) measures.
Genetic Markers for Detecting the DNA of Pathogenic Borrelia
Authors: Khammadov N. I. , Khamidullina A. I. 
Published: July 2022

The aim of the study was to analyze the genetic markers of Lyme disease pathogens, which can be used to specifically indicate maximum number of their strains and isolates. Materials and methods. The nucleotide sequences of various genes of Borrelia garinii, B. afzelii, B. burgdorferi were downloaded from the NCBI database (National Center for Biological Informatization). The occurrence of the analyzed nucleotide sequences in the genetic code of various organisms was determined in the nBLAST software utility. For the design of primers and probes, the Vector NTI 9.1.0 program (“Invitrogen Corporation”, Carlsbad, USA) was used. DNA was isolated using the MAGNO-sorb kit, version 100-200 (“AmpliSens”, Moscow, Russia), according to the manufacturer’s instructions. Primers and probes were synthesized at “Evrogen” company (Moscow, Russia). For PCR, reagents manufactured by “Synthol” company (Moscow, Russia) were applied.Results and discussion. In order to perform the reliable indication of pathogenic Borrelia, specific loci (genes) of B. garinii, B. afzelii, B. burgdorferi, which were significantly different from the genetic code of other representatives of the genus Borrelia and from the DNA of other organisms, have been determined by molecular-genetic methods. As a result of a preliminary determination of the analytical significance of the studied loci, the following genes and loci were selected for further work: pepX, clpA, ospA, p83/100, ospC and flaB, of which the flaB and ospA genes were selected for practical indication of pathogenic Borrelia DNA. The genetic markers of B. burgdorferi and B. afzelii are displayed during amplification of the flaB gene, while B. garinii and B. afzelii occur when the ospA gene is used as a genetic marker.
Efficiency of Using the Hydrogen Peroxide Vapor Generator “Fhileas 75” for Disinfection of the Air Ducts of separately Ventilated system for Infected Animal Housing
Authors: Germanchuk V. G. , Kislitsina E. V. , Lobovikova О. A. , Mironova N. P. , Shavina N. Yu. , Gordeeva M. V. 
Published: July 2022

The aim of the work was to evaluate the efficiency of using the “Fhileas 75” hydrogen peroxide vapor generator for decontaminating the air ducts of the individually ventilated system, “Bio A.S.”, for housing of infected animals.Materials and methods. The hydrogen peroxide vapor generator “Fhileas 75” (France), a disinfectant manufactured by “FHILEASAFE” (7 % hydrogen peroxide solution and 0.15 % peracetic acid solution), separately ventilated system “Bio A.S.” (Germany) for the infected animal housing were applied in the work. Serratia marcescens 9 was used as test-culture.Results and discussion. The efficiency of using the hydrogen peroxide vapor generator “Fhileas 75” for decontamination of air ducts and internal surfaces of the rack of the individually ventilated system “Bio A.S.” on the test-culture S. marcescens 9 at 1·106 mc/ml concentration has been established (operation parameters of the individually ventilated system unit are as follows: air exchange rate – 60 changes per hour, air flow volume – 28 m3/hour, number of disinfection cycles – 5, disinfectant spraying time – 97 min, exposure time – 24 hours).
Viability of sARs-CoV-2 Coronavirus strains on Different types of test surfaces, in Drinking Water and Their Resistance to Disinfectants
Authors: Zolin V. V. , Os’kina O. P. , Solodky V. V. , Ovchinnikova A. S. , Agafonov A. P. , Maksyutov R. A. 
Published: July 2022

The purpose of the research was to study the dynamics of residual infectious activity of SARS-CoV-2 virus strains belonging to different genovariants, on different types of surfaces, in samples of drinking dechlorinated water at 24–28 °C, as well as their resistance to disinfectants.Materials and methods. The studies were carried out using SARS-CoV-2 coronavirus strains obtained from the State Collection of Causative Agents of Viral Infectious Diseases and Rickettsiosis, which operates at the premises of the SSC VB “Vector”. The evaluation of the residual infectivity of the SARS-CoV-2 coronavirus was carried out through titration of samples in cell culture.Results and discussion. The conducted studies have confirmed the ability of all investigated strains of the SARS-CoV-2 coronavirus to maintain their infectious activity at 24–28 °C on most of the examined types of test surfaces for at least 48 hours, while the virus is best preserved on stainless steel and plastic. All studied strains of the SARS-CoV-2 coronavirus are viable in drinking dechlorinated water for at least 48 hours. In addition, it has been found that all of them are sensitive to disinfectants of different groups, widely used for disinfection when working with pathogenic biological agents or for treating hands and surfaces contaminated with viruses. Chlorine-containing disinfectants are the most active. Skin antiseptics based on ethyl and isopropyl alcohols are suitable for disinfecting hands and objects contaminated with the SARS-CoV-2 virus.
Quality of life in aging process: the role of work-related psychosocial conditions and social support
Authors: Gaspar Tania 
Published: July 2022

Aging has been a growing source of increased opportunities raised by longevity, but also a growing source of concern,particularly with regard to the mental health and the well-being of elderly people. As such, it is highly relevant to have a better understanding of the relationship between workplace related psychosocial factors and quality of life, not only among working population, but also among retired individuals.
The main objective of this study is to better understand and characterize the relation between quality of life (QoL), work-related psychosocial conditions, and social support for both working and retired adults. Participants were 1.330, 62.2% of which female, with ages ranging between 55 and 75 years old. 47% of the respondents were working and 46 % were retired. Three structural equation models were tested, using the total sample and by labor situation (working and retired). The results showed that the stress management (with a greater effect in retired participants) and the social support (with a greater effect in working participants) have a positive influence on the QoL factor. Better work-related psychosocial conditions and higher social support were predictors of higher/better QoL. Our study allowed us to conclude that QoL is influenced by working situation, social support and work-related psychosocial conditions. This is a powerful message to policy makers in view of the growth of older populations.
Vidangadi Lauha’ for Obese Type 2 Diabetes mellitus; Randomised controlled clinical study
Authors: Khobarkar Punam , Gulhane Jayant , Nakanekar Amit 
Published: July 2022

Abstract
Background Type 2 diabetes mellitus in obese persons is becoming alarming due to the increasing prevalence of its microvascular and macro vascular complications. Multitargeted treatment can be considered better than single targeted treatment in view of multiple pathways involved in the pathogenesis of diabetes and its complications.Methodology-This open-label randomised controlled clinical study aimed to evaluate the clinical efficacy of ‘Vidangadi Lauha’ (an Ayurveda formulation) in comparison with metformin for obese type II diabetes mellitus. Participants were divided into two groups. The trial group received Vidangadi Lauha 5 gm BID, and the control group received tablet metformin 500 mg BID for a duration of 3 months.Results- Among 550 screened participants, 120 participants were eligible; out of them, 100 participants were enrolled and randomised by a computer-generated method, and of them, 80 patients (40 in each group) completed the trial. Vidangadi Lauha showed a reduction in HbA1c from 8.048 (0.95) to 7.14 (0.73), while metformin showed a reduction in HbA1C from 8.3 (0.99) to 7.18 (0.67).Conclusion-Both treatments were equally effective in reducing blood sugar fasting (F), postmeal (PM) glycated haemoglobin (HbA1C) and body mass index (BMI). Vidangadi Lauha is more effective in reducing Ayurvedic symptoms, waist hip ratio and cholesterol than metformin. High-density lipoproteins (HDLs) were improved by minor clinical differences in both groups. Neither treatment had a statistically significant effect on reducing low-density lipoproteins (LDL).
Analysis of the differential expression and prognostic relationship of DEGs in AML based on TCGA database
Authors: Gao Yue , Jia Yinnong , Yu Zhengmin , Ji Xinyu , Liu Xiaowen , Han Lei , Zhang Hengdong , Zhu Baoli , Xu Ming 
Published: July 2022

Abstract
Background: Acute myeloid leukemia (AML) is a common and lethal haematological malignant hyperplastic disease originating from hematopoietic stem cells. The purpose of this study is to obtain the key differentially expressed gene (DEG) related to the survival of AML by The Cancer Genome Atlas (TCGA) database and to verify these genes by a clinical follow-up investigation, in order to identify valuable predictive and prognostic biomarkers for early diagnosis of AML and predict the survival rates.Methods: The RNA sequencing (RNA-Seq) data and clinical information of TCGA-LAML were downloaded from the TCGA database. After that we 1) screened the survival related DEGs by Cox regression analysis, 2) selected the cytogenetics risk related DEGs by DESeq2 R package, 3) and filtrated the genes in the top10 pathways of up-regulated and down-regulated of Normalization Enrichment Score (NES) by Gene Set Enrichment Analysis (GSEA). Finally, we focused the intersectional genes of above three parts as the key gene of our present study. The following Multivariate Cox regression analyses were performed to analyze these intersectional genes as independent factors. Proportional hazards assumption was evaluated to test Cox regression model by Schoenfeld residuals. The Kaplan–Meier survival curve and Nomogram were plotted to predict and compare the 1-year, 3-year, and 5-year survival rates of AML patients. The concordance index (C-index) and calibration curve were used to evaluate the prediction performance of nomogram.Results: A total of 151 RNA-Seq samples for 60488 genes and 200 clinical samples were selected from the TCGA database. 1005 survival related DEGs were obtained by Cox regression analyses (P<0.05, exp (coef)>1), 2291 cytogenetics risk category related DEGs were identified using DESeq2 (log2 Fold Change>1 and P<0.05), and 790 meaningful pathways of cytogenetics risk category were selected from GSEA (abs(NES)≥1, NOM p-val≤0.05 and FDR q-val≤0.25). Screened a key gene IGHM that is related to patient survival rate. The results showed that the IGHM expression and age displayed statistical associations with the survival time of patients (P<0.05). The risk of death in the patients with high expression group of IGHM was 2.07 times higher than those in low expression group (P<0.05). For survival, the 1, 3, and 5-year survival of patients in the high expression group are predicted to be 68%, 43%, and 30%. GSEA analysis revealed that IGHM were mainly enriched in the immune response (BP).Conclusion: High expression of IHGM gene is an independent risk factor for the prognosis of patients with AML and can be an important molecular marker for predicting the prognosis of patients with AML.
Targeting the Post-Synaptic Proteome in Alzheimer Disease with Psychosis
Authors: Krivinko Josh M , DeChellis-Marks Michael , Zeng Lang , Fan Peihao , Lopez Oscar L. , Ding Ying , Wang Lirong , Kofler Julia , MacDonald Matthew L. , Sweet Robert A. 
Published: July 2022

Abstract
INTRODUCTION: Individuals with Alzheimer Disease who develop psychotic symptoms (AD+P) experience more rapid cognitive and functional decline, and have reduced indices of synaptic integrity, relative to those without psychosis (AD-P). We hypothesized that the postsynaptic density (PSD) proteome is altered in AD+P relative to AD-P, and that this proteomic signature could be used to nominate novel pharmacotherapies. METHODS: Liquid-Chromatography/Mass Spectrometry analysis of PSDs from dorsolateral prefrontal cortex of AD+P, AD-P, and cognitively normal elderly subjects. RESULTS: The PSD proteome signature of AD+P was characterized by lower levels of a network of kinases, proteins regulating Rho GTPases, and other regulators of the actin cytoskeleton. Potential novel therapies identified included the C-C Motif Chemokine Receptor 5 inhibitor, maraviroc. DISCUSSION: AD+P is characterized by broad changes in the PSD proteome. Further testing of potential therapies for their ability to reverse these changes and reduce psychotic-like behaviors in model systems is warranted.
Neural-network-based reduced order modeling for nonlinear vertical sloshing with experimental validation
Authors: Pizzoli Marco , Saltari Francesco , Coppotelli Giuliano , Mastroddi Franco 
Published: July 2022

Abstract
In this paper, a nonlinear reduced order model based on neural networks is introduced in order to model vertical sloshing in presence of Rayleigh-Taylor instability of the free surface for use in fluid-structure interaction simulations. A box partially filled with water, representative of a wing tank, is first set on vertical harmonic motion via a controlled electrodynamic shaker. Accelerometers and load cells at the interface between the tank and an electrodynamic shaker are employed to train a neural-network-based reduced order model for vertical sloshing. The model is then investigated for its capacity to consistently simulate the amount of dissipation associated with vertical sloshing under different fluid dynamics regimes. The identified tank is then experimentally attached at the free end of a cantilever beam to test the effectiveness of the neural network in predicting the sloshing forces when coupled with the overall structure. The experimental free response and random seismic excitation responses are then compared with that obtained by simulating an equivalent virtual model in which the identified nonlinear reduced-order model is integrated to account for the effects of violent vertical sloshing.
Pneumococcal Vaccine Schedules (PVS) study: a cluster-randomised, non-inferiority trial of an alternative versus standard schedule for pneumococcal conjugate vaccination: Statistical Analysis Plan
Authors: Mackenzie Grant Austin , Palmu Arto A , Jokinen Jukka , Osei Isaac , Flasche Stefan , Greenwood Brian , Mulholland Kim , Nguyen Cattram 
Published: July 2022

Abstract
RationaleThe effectiveness of universal immunisation with pneumococcal conjugate vaccine (PCV) has been evident in many countries. However, the global impact of PCV is limited by its cost, which has prevented its introduction in several countries. Reducing the cost of PCV programmes may facilitate vaccine introduction in some countries and improve the sustainability of PCV in EPIs in low-income countries when they transition away from subsidised vaccine supply.Methods and designPVS is a real-world field trial of an alternative schedule of one dose of PCV scheduled at age 6 weeks with a booster dose at age 9 months (i.e. the alternative ‘1+1’ schedule) compared to the standard schedule of three primary doses scheduled at 6, 10, and 14 weeks weeks of age (i.e. the stardard ‘3+0’ schedule). Delivery of the interventions began in late 2019 in 68 geographic clusters and will continue for 4 years. The primary endpoint is prevalence of nasopharyngeal vaccine-type pneumococcal carriage in children aged 2-260 weeks with clinical pneumonia in Year 4. Secondary endpoints are prevalence of vaccine-type pneumococcal carriage among all ages in Year 4 and incidence of radiological pneumonia in children enrolled to receive the interventions. Additional disease and carriage endpoints are included.PurposeThis statistical analysis plan (SAP) describes the cohorts and populations, and follow-up criteria, to be used in different analyses. The SAP defines the endpoints and describes how adherence to the interventions will be presented. We describe how analyses will account for the effect of clustering and stratified randomisation. The SAP defines the approach to non-inferiority and other analyses. Defining the SAP early in the trial will avoid bias in analyses that may arise from prior knowledge of trial findings.
Success Factors of Adaptation of Newly Graduated Nurses: A Scoping Review
Authors: Baharum Hafidza , Ismail Aniza , McKenna Lisa , Mohamed Zainah , Ibrahim Roszita , Hassan Nor Haty 
Published: July 2022

Abstract
Background: Difficulties in adapting to the workplace can affect the transition of new nurses. They must adapt quickly, as this can impact their future career prospects. Therefore, this review aimed to identify success factors that promote effective transition and new nurses’ adaptation. Methods: The study adopted Joanna Briggs Institute methodology for scoping reviews. Data were collected from MEDLINE, Scopus, EBSCOhost, and Web of Science publications between 2011 and 2020. A total of 23 articles were included. The study included qualitative or quantitative primary research studies focusing on contributing factors to helping new nurses adapt to their work environment during the transition period. Thematic analysis was used to identify key emerging themes.Results: Three main themes were identified: (1) Organisational contribution (comprising social development, organisational culture, work characteristics, work readiness, work commitment, and professional role), (2) Personality traits (encompassing self-embodiment, masking personality, being proactive and confident), and (3) Academic institutions (comprising pre-entry knowledge and role of nursing faculty). The process of adaptation for new nurses needs to start during nursing education, supported by the workplace organisation and also driven by the nurse’s own personality. The review identified that the role of nursing education in helping to provide students with the knowledge and actual clinical experiences has a profound effect on developing nurses’ levels of self-confidence in delivering nursing care effectively. In addition, a warm environment supports nurses emotionally and physically. Conclusions: Though organisations and educational institutions have taken numerous efforts to ensure that new nurses have adequate support, personality and values are also equally important to ease adaptation during the transition process. This knowledge should be applied and emphasised in academic and workplace programs designed for newly graduated nurses to develop and strengthen their personalities and values, especially in increasing confidence levels and promoting proactive values that facilitate new nurses to adapt to their new employment quickly and effectively.