bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Allosterically Modulated Free Fatty Acid Receptor 2 is Transactivated by an Increase in the Cytosolic Concentration of Calcium Ions
Authors: Lind Simon , Wu Yanling , Sundqvist Martina , Forsman Huamei , Dahlgren Claes 
Published: October 2022

AbstractAllosterically modulated free fatty acid receptor 2 (FFA2R/GPR43) can be activated without the involvement of any orthosteric FFA2R agonist, by signals generated for example by P2Y2R, the G protein coupled receptor for ATP. An FFA2R specific positive allosteric modulator (PAM; Cmp58) was used to disclose the molecular mechanism by which signals generated by ATP/P2Y2R transactivates FFA2R. The P2Y2R induced signal that transactivates the allosterically modulated FFA2R was generated downstream of the Gαqcontaining G protein that couple to P2Y2R. A receptor induced rise in the cytosolic concentration of ionized calcium ([Ca2+]i) was hypothesized to be the receptor transactivation signal. The Gαqdependent transient rise in [Ca2+]iinduced by the ATP activated P2Y2Rs was not affected by Cmp58. The hypothesis gained, however, support from the finding that the modulator transferred FFA2R to a Ca2+sensitive state. The rise in [Ca2+]iinduced by the Ca2+specific ionophore ionomycin, activated the allosterically modulated FFA2R. The response induced by ionomycin was rapidly terminated and the FFA2Rs could then no longer be activated by the orthosteric FFA2R agonist propionate or be transactivated by the signal generated by the activated ATP receptor. The desensitized/non-responding state of FFA2R was, however, revoked by an earlier described cross-sensitizing/activating allosteric FFA2R modulator. The receptor transactivation of the allosterically modulated FFA2Rs, represent a unique regulatory receptor cross-talk mechanism by which the activity of a G protein coupled receptor is controlled by a signaling system operating from the cytosolic side of the plasma membrane.One-sentence summaryA P2Y2receptor signal generated downstream of a Gαq containing G protein transactivates the allosterically modulated FFA2 receptor
SLICER: Seamless Loss of Integrated Cassettes Using Endonuclease Cleavage and Recombination inDeinococcus radiodurans
Authors: Brumwell Stephanie L. , Van Belois Katherine D. , Nucifora Daniel P. , Karas Bogumil J. 
Published: October 2022

ABSTRACTMethods for creating seamless genome modifications are an essential part of the microbial genetic toolkit that allows for strain engineering through the recycling of selectable markers. Here, we report the development of a method, termed SLICER, which can be used to create seamless genome modifications inD. radiodurans. We used SLICER to sequentially target four putative restriction-modification (R-M) system genes, recycling the same selective and screening markers for each subsequent deletion. A fifth R-M gene was replaced by a selectable marker to create a finalD. radioduransstrain with 5 of the 6 putative R-M systems deleted. While we observed no significant increase in transformation efficiency, SLICER is a promising method to obtain a fully restriction-minus strain and expand the synthetic biology applications ofD. radioduransincluding as anin vivoDNA assembly platform.GRAPHICAL ABSTRACT
The impact of social media technologies on organization cultural intelligence and new product development in international markets
Authors: Hazzam Joe , Wilkins Stephen , Strong Carolyn 
Published: October 2022

PurposeThe study examines the role of social media technologies (SMTs) as a driver of organization cultural intelligence (OCI) and new product development (NPD) capabilities, and how the complementary effects of these capabilities contribute to multinational corporations (MNCs)’ performance. Further, the study investigates the capability–performance relationship under conditions of high and low market and technological turbulence.Design/methodology/approachA quantitative survey method was implemented, with the data provided by senior marketing managers employed in MNC regional offices. The proposed model was tested using structural equation modeling and multi-group moderation analysis, and fuzzy-set qualitative comparative analysis (fsQCA).FindingsThe results indicate that SMTs support the development of OCI and NPD capabilities, which in turn contribute to MNC regional performance. A high level of technological turbulence only weakens the relationship between OCI and performance.Research limitations/implicationsThe results suggest that OCI contributes to MNCs’ performance, by deploying social media information and complementing the organization’s NPD capability under a specific environmental context.Practical implicationsThe paper offers practical recommendations to MNCs on social media use when developing and launching new products in different regional markets. MNCs need to recruit culturally intelligent managers, who consider the level of market and technological turbulence when combining several types of capabilities.Originality/valueWithin the dynamic marketing capabilities literature, this is the first study to incorporate and reliably measure cultural intelligence capability. The research offers empirical evidence that OCI and NPD capabilities are necessary to achieve superior MNC performance and depend on the level of market and technological turbulence.
Dynamic brand positioning: a firm-customer synergistic strategy of brand meaning cocreation in a hyperconnected world
Authors: He Jiaxun , Zhang Fan 
Published: October 2022

Purpose
This study aims to explore how cocreated brand meaning builds and affects dynamic brand positioning in a hyperconnected world.

Design/methodology/approach
The authors conducted a qualitative study of Casarte, a high-end appliance brand, as an instrumental case for conceptualizing and theorizing. This study constructs a matrix of dynamic brand positioning as the key analysis framework using in-depth interview data, firm materials and user-generated content from online brand communities.

Findings
The matrix of dynamic brand positioning has two dimensions: brand core and peripheral meaning, and firm- and customer-led orientation. The interaction between the firm and its customers strengthens the understanding of a brand’s core meaning and consistency perception, expands the scope of brand peripheral meaning and improves the perception of brand meaning diversity. The mutual transformation of the ambidexterity of core and peripheral meanings facilitates the dynamic positioning of brands.

Research limitations/implications
This study is a qualitative case study; the relevant conclusions have not been tested empirically. If longitudinal data of actual tracking support the effect of dynamic brand positioning, the theory’s reliability can be more rigorously tested.

Practical implications
It provides managerial logic and a tool for firms to practice dynamic brand positioning in a hyperconnected world, which contributes to the implementation of the emerging firm-customer synergistic strategy.

Originality/value
This study proposes a construct of dynamic brand positioning supported by qualitative evidence. It disputes the traditional view that brand positioning is determined by the perception of core meaning consistency and creatively puts forward the view that brand positioning evolves dynamically with the mutual transformation of the ambidexterity of brand core meaning and peripheral meaning.
Launching the dynamic employee engagement framework: towards a better understanding of the phenomenon
Authors: Davis Amanda S. , Van der Heijden Beatrice I.J.M. 
Published: October 2022

Purpose The dynamic employee engagement framework visually delineates employee engagement from its antecedents and responds to the debates regarding how employee engagement is positioned within the academic literature.Design/methodology/approach A narrative literature review was conducted.FindingsThe new framework visually demonstrates the impact of contextual changes on employee engagement. Additionally, it positions employee engagement as a psychological state (psychological presence) and as a behaviour.Research limitations/implicationsThe new framework promotes the study of behavioural engagement when the incorporation of context and/or multiple voices is required. Accordingly, studying behavioural engagement may help scholars understand more about the dynamics of employee engagement within and across different settings, reduce the reliance on self-reported studies and help bridge the employee engagement academic and practitioner discourses.Practical implications Human resource management/development practitioners and line managers may use the new structured framework in several ways to help them foster employee engagement.Originality/value The new framework extends five integrated employee engagement models by incorporating the macro and micro external context, and repositioning trust and feedback. In doing so, it more accurately conveys diagrammatically the dynamic nature of employee engagement following significant contextual changes. It also visually separates out the antecedents to employee engagement thus respecting construct boundaries and positions employee engagement as a multi-dimensional construct comprised of psychological state (psychological presence) and behavioural engagement. Building on this, we also argue that double-loop learning is an example of behavioural engagement.
Effect of the geometric parameter difference of multilayer structural transfer film on the wear of PTFE-based composites
Authors: Zhang Longxiao , Xie Ting 
Published: October 2022

Purpose
The purpose of this paper is to explore the geometric parameter difference of the terrace-like structural transfer film under different working parameters [pressure and velocity (PV) values] and filled particle types (three fillers: SiO2, TiO2 and ZnO), and find the geometric parameter related to the wear of polytetrafluoroethylene (PTFE)-based composites.

Design/methodology/approach
PTFE composites were filled with SiO2, TiO2 and ZnO particles, and the morphology parameter of the PTFE composite transfer film under different PV values obtained from the rotary reciprocating pin-on-disk frictional tester was quantified by using a three-dimensional laser scanning microscope.

Findings
The results showed that the effective layer coverage rate and effective thickness of the transfer film had a good relationship with the wear of the three PTFE composites. On the whole, increasing the speed or load was helpful to increase the effective thickness of the three PTFE composite transfer films, but reduced the effective layer coverage rate. The greater the effective layer coverage rate and effective thickness of the transfer film, the better the wear resistance of the PTFE composites in the entire speed and load range.

Originality/value
This work will promote further understanding of the transfer film and lay a foundation for realizing its morphology regulation and improving the wear of the PTFE composites.
Spatial pattern of burglary in residential neighbourhoods: a case study of Abuja, Nigeria
Authors: Iliyasu Idris Isah , Abdullah Aldrin , Hedayati Marzbali Massoomeh 
Published: October 2022

Purpose
Abuja, the capital city of Nigeria, is one of the fastest growing capital cities in sub-Saharan Africa. Recently, the city is experiencing an alarming rate of burglary and violent crimes, while the city planning management frameworks lacks adequate and effective crime mapping, monitoring and management techniques necessary for achieving liveable and safe environment for habitation despite its grandiose spatial planning and aesthetically appealing architectural design as a modern city. Based on police crime records (2007–2018) and geospatial analysis, this paper aims to provide adequate understanding on the interplay of land use configuration and burglary crime formation in residential neighbourhoods of Abuja, Nigeria.

Design/methodology/approach
The methods used for the purpose of data collection includes; field survey, Block Environmental Inventory, while inferential statistics and Geographic Information System tools was used for data analysis. The analysis established that Lagos, Nsukka and Enugu Streets are hotspots; while Chief Palace street, Ladoke Akintola and Oka-Akoko streets were found to be cold spots.

Findings
This study, however, established the applicability of crime pattern, opportunity theory and routine activity theory in understanding the rising burglary crime events in the study areas and the link between physical characteristics of street block typology and burglary crime pattern. The results of the analysis has in a way affirmed the positions of the theories, while disagreed with them in cases where the results indicated contrary outcome.

Originality/value
This paper concluded with inference drawn from the results that supported mixed-use development but with built-in crime prevention through environmental design strategies as effective burglary crime prevention mechanisms that contribute to crime rate reduction.
Open strategizing and accelerated internationalization process in different contexts
Authors: Bellucci Christiane , Lavarda Rosalia Aldraci Barbosa , Floriani Dinorá Eliete 
Published: October 2022

PurposeDue to the changes in organizational, social, cultural and technological factors, companies from different contexts are shifting towards open forms of strategy-making with more widened inclusion of internal and external actors and greater transparency regarding their strategic issues, including their internationalization processes. The purpose of this paper is to understand how Open Strategizing occurs in the accelerated process of internationalization considering different contexts.Design/methodology/approachThe authors conducted a qualitative comparative case study in Brazilian and English technology-based small and medium-sized enterprises (SMEs) which rapidly internationalized. Furthermore, data was collected through semi-structured interviews, observations and documental analysis.FindingsThe authors suggest that openness contributes to the accelerated process of internationalization. Additionally, the authors show that the home-country and the national cultural contexts affect openness. The authors also disclose openness as crucial and inherent to the accelerated process of internationalization, while context is relevant but not determinant in the Open Strategizing.Originality/valueThe authors contribute to Open Strategy (OS) literature by presenting how Open Strategizing unfolds daily during the internationalization process and by evidencing the role of home-country and national cultural contexts in the configuration and dynamics of Open Strategizing. The authors also contribute to the international entrepreneurship (IE) literature by advancing the understanding of the strategies and drivers adopted by technology-based SMEs internationalizing in an accelerated way.
Impact of workplace bullying on knowledge hiding: The mediating role of emotional exhaustion and moderating role of workplace friendship
Authors: Islam Talat , Chaudhary Arooba 
Published: October 2022

PurposeWorkplace bullying has become a major challenge across the globe as it is associated with negative outcomes. Therefore, this study was designed to assess the impact of workplace bullying on knowledge hiding through emotional exhaustion. The study further investigated the moderating role of workplace friendship to reduce the detrimental effect of workplace bullying on knowledge hiding.Design/methodology/approachThe study collected data from 358 nurses working in three large cities (Karachi, Islamabad and Lahore) of Pakistan in two lags to tackle the issue of common method bias.FindingsThe study applied structural equation modeling with maximum likelihood method using AMOS to test the hypotheses. The study noted that workplace bullying elevates emotional exhaustion and knowledge hiding among nurses; whereas, workplace friendship was noted as an essential factor to control adverse effects of workplace bullying on knowledge hiding.Research limitations/implicationsThe study used a cross-section design that restricts causality. However, the findings of this study add to the conservation of resources theory by providing insights into the role of workplace friendship in reducing the relationship between workplace stressors (bullying) and negative behaviors (knowledge hiding). The study also suggests healthcare administration foster workplace friendship to cope with the negative outcomes of workplace bullying.Originality/valueDrawing upon conservation of resources, this study explored the moderating role of workplace friendship between workplace bullying and knowledge hiding.
Novel approaches to energize microbial biocatalysts
Authors: Durante‐Rodríguez Gonzalo , Carmona Manuel , Díaz Eduardo 
Published: October 2022

AbstractAn efficient and cheap energization of microbial biocatalysts is essential in current biotechnological processes. A promising alternative to the use of common organic or inorganic electron donors is the semiconductor nanoparticles (SNs) that absorb light and transfer electrons (photoelectrons) behaving as artificial photosynthetic systems (biohybrid systems). Excited photoelectrons generated by illuminated SNs are highly reductive and readily accepted by membrane‐bound proteins and electron shuttles to drive specific cell reduction processes and energy generation in microbes. However, the operational mechanisms of these hybrid systems are still poorly understood, especially at the material–microbe interface, and therefore the design and production of efficient biohybrids are challenging. Some major limitations/challenges and future prospects of SNs as microbial energization systems are discussed.
Case report: Successful treatment with tofacitinib and colchicine in a patient with Schnitzler syndrome
Authors: Li Man , Chen Yu‐Wen , Shen Amy , Xie Yong‐Xin , Wu Yonglan , Zeng Xianlin , Leong Pui‐Ying 
Published: October 2022

AbstractWe report a case of late‐onset Schnitzler syndrome successfully treated with Janus‐activated kinase (JAK) inhibitors and colchicine. Schnitzler syndrome should be considered for recurrent chronic urticaria when accompanied by fever, fatigue, rapid weight loss, and poor response to antihistamine treatment. Skin biopsy, bone marrow biopsy, and electrophoresis help confirm the diagnosis. Early diagnosis and treatment can lead to complete resolution of symptoms. Besides interleukin (IL)‐1 and IL‐6 inhibitors, JAK inhibitors and colchicine may be considered as other choices of treatment.
Cultural identity affiliation and alcohol use and related consequences among American Indian and White adolescents: A latent profile analysis
Authors: Schick Melissa R. , Egan Alana , Crawford Michael , Nalven Tessa , Goldstein Silvi C. , Spillane Nichea S. 
Published: October 2022

AbstractBackgroundAdolescent alcohol use is a significant public health concern and rates of alcohol use are higher among American Indian (AI) adolescents than national samples of non‐AI youth. A potential factor in understanding AI alcohol use is cultural identity, which can vary widely based on experiences of historical trauma. We used latent class analysis to examine cultural identity in AI and White adolescents and their alcohol use outcomes in relation to the latent class solutions.MethodsThe samples included 3189 AI adolescents (Mage = 14.76, 48.9% female) and 1579 White adolescents (Mage = 15.56, 48.7% female) living on or near a reservation. Participants completed self‐report measures of AI and White cultural identity affiliation, alcohol use, and alcohol‐related problems. We examined (1) the best‐fitting latent class solution with respect to American Indian (AI) and White cultural identity; (2) equivalence of the latent class solution; and (3) alcohol use outcomes across the optimal latent class solution.ResultsLatent profile analyses indicated an optimal 3‐class solution in both the AI and White samples, which differed by level of affiliation with AI and White cultural identity. While the optimal number of classes were similar across racial groups (configural profile similarity), the nature of the classes differed (structural profile dissimilarity). The three classes represented low overall scores on AI and White cultural identity (Marginalized), a mixture of high and low scores on AI and White cultural identity (Third Culture), and overall high scores on AI and White cultural identity (Bicultural). Alcohol‐related problems predicted membership in the Third Culture class compared with the Marginalized class and the Bicultural class. Specifically, youth in the Third Culture class reported significantly fewer alcohol‐related problems than youth in the Marginalized and Bicultural classes. Alcohol use did not predict latent class membership.ConclusionsThe future‐oriented nature of the Third Culture class may provide protection against adverse alcohol‐related outcomes. Research is needed to test interventions that target greater future orientation and future plans to integrate culture into adolescents' lives.
Depressive symptoms and mortality‐findings from Helsinki birth cohort study
Authors: Eriksson Mia D. , Eriksson Johan G. , Korhonen Päivi , Koponen Hannu , Salonen Minna K. , Mikkola Tuija M. , Kajantie Eero , Wasenius Niko S. , von Bonsdorff Mikaela , Kautiainen Hannu , Laine Merja K. 
Published: October 2022

AbstractBackgroundIndividuals with depression and depressive symptoms have a higher mortality rate than non‐depressed individuals. The increased comorbidity and mortality associated with depression has remained largely unexplained. The underlying pathophysiological differences between depressive subtypes, melancholic and non‐melancholic, may provide some explanation to this phenomenon.MethodsOne thousand nine hundred and ninety five participants (mean age 61 years) from the Helsinki Birth Cohort Study were recruited for this prospective study and followed up for a mean of 14.1 years. Information regarding medical history, lifestyle, and biochemical parameters were obtained. Depressive symptoms were assessed using the Beck Depression Inventory. Standardized mortality ratios were calculated.ResultsParticipants were followed up for a total of 28,044 person‐years. The melancholic depressive group had an increased adjusted risk of mortality [HR 1.49 (95% CI: 1.02–2.20)] when compared to the non‐depressive group. Comparing mortality to the whole population of Finland using standardized mortality ratios (SMR) both the non‐melancholic [1.11 (95% CI: 0.85–1.44)] and melancholic depressive [1.26 (95% CI: 0.87–1.81)] groups had higher mortality than the non‐depressive group [0.82 (95% CI: 0.73–0.93)].ConclusionsMelancholic depressive symptoms are most strongly related to a higher mortality risk.