bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of two-qubit quantum nonlocality in a Heisenberg chain model with the intrinsic decoherence
Authors: Mohamed A.-B. , Aldosari F. M. , Eleuch Hichem 
Published: October 2022

Abstract
This paper investigates the dynamics of two-spin nonlocality generation in a Heisenberg XXX chain with Dzyaloshinskii-Moriya (DM) and Kaplan-Shekhtman-Entin-Wohlman-Aharony (KSEA) interactions. We analyze the two-spin nonlocality dynamics by using uncertainty-induced nonlocal-ity, maximal Bell inequality, and log-negativity. We demonstrate that a separable two-spin Heisen-berg XXX chain state, induced by two-spin antiferromagnetic interaction as well as x-component of DM and KSEA interactions, could evolve to maximal two-spin nonlocality state. The ability of preserving the maximal uncertainty-induced nonlocality can be enhanced by increasing the coupling strength of the spin-spin interaction coupling. The hierarchy principle is maintained for the two-spin Bell nonlocality and log-negativity entanglement. The two-spin log-negativity dynamics exhibits the phenomenon of sudden death and birth as a result of the intrinsic decoherence, which also causes a reduction in the two-spin nonlocalities. The two-spin uncertainty-induced nonlocality is more robust , against the intrinsic decoherence, than the other types of the nonlocality. The results indicate that by boosting the two-spin antiferromagnetic interaction, the produced nonlocalities (resulting from the DM and KSEA x-component interactions) can be shielded from the intrinsic decoherence effect.
Identifying Factors Affecting Depressive Symptoms and Incidence of Mental Health Diagnosis within 1 Year among 2SLGBTQ+ Youth During COVID-19 Using Machine Learning Methods
Authors: Dharma Christoffer , Grace Daniel , Logie Carmen , Abramovich Alex , Mitsitakis Nicholas , Baskersville Bruce , Chaiton Michael 
Published: October 2022

Abstract
Purpose: There is a lack of longitudinal studies that examine changes in mental health among 2SLGBTQ+ youth during the COVID-19 pandemic. Hence, using a Canadian cohort of young 2SLGBTQ+ (16-29 years old), weidentified the factors that predicted increases in self-reported depressive symptoms scores (n = 882) and those that predicted incidence in diagnosis of mental health problems within 1 year among a subset of the cohort who were never diagnosed with mental health problems at baseline (n = 344) using machine learning techniques.
Methods: Random Forest regression and classifier were used to identify factors associated with the outcomes. Data were split into training and test sets; Root Mean Square Error (RMSE) and area under the curve (AUC) were used as the criteria to evaluate model performance on the test set.
Results: The top ten predictors of each outcome were identified, nine of them were the same for both outcomes. These shared nine variables were: self-rated mental health (SRMH), adverse childhood experiences (ACE), depressive symptoms, stressful life, internalized homophobia, outness, community connectedness, enacted stigma, and perceived sexual stigma at baseline. Some predictors had a unique non-linear relationship with the outcomes.
Conclusion: These analyses suggest that 2SLGBTQ+ specific factors (such as outness) and one’s psychological well-being were the most important factors in predicting one’s future mental health. Social identities such as gender or sexuality appeared to be less significant in affecting one’s mental health. More studies with larger samples are needed to better understand some of the complex non-linear associations.
Leveraging propionate-induced growth inhibition in Corynebacterium glutamicum to evolve improved methylmalonyl-CoA-dependent polyketide production
Authors: Keasling Jay , zhan chunjun , Lee Namil , Lan Guangxu , Dan Qingyun , Cowan Aidan , Wang Zilong , Baidoo Edward , Kakumanu Ramu , Luckie Bridget , Kuo Rita , McCauley Joshua , Haushalter Robert 
Published: October 2022

Abstract
Corynebacterium glutamicum is a promising host for production of valuable polyketides. Propionate addition, a strategy known to increase polyketide production by increasing intracellular methylmalonyl-CoA availability, causes growth inhibition in C. glutamicum. The mechanism of this inhibition was unclear prior to our work. Here we provide evidence that accumulation of propionyl- and methylmalonyl-CoA induces growth inhibition in C. glutamicum. We then show that growth inhibition can be relieved by introducing methylmalonyl-CoA-dependent polyketide synthases. With germicidin as an example, we used adaptive laboratory evolution (ALE) to leverage the fitness advantage of polyketide production in the presence of propionate to evolve improved germicidin production. Whole genome sequencing revealed mutations in germicidin synthase (Gcs), which improved germicidin titer, as well as mutations in citrate synthase, which effectively evolved the native glyoxylate pathway to a new methylcitrate pathway. Together, our results show that C. glutamicum is a capable host for polyketide production, and we can take advantage of propionate growth inhibition to drive titers higher by evolution.
Laser Printed Microelectronics
Authors: Yang Liang , Hu Hongrong , Scholz Alexander , Feist Florian , Marques Gabriel , Kraus Steven , Bojanowski N. Maximilian , Blasco Eva , Barner-Kowollik Christopher , Aghassi-Hagmann Jasmin , Wegener Martin 
Published: October 2022

Abstract
Printed organic and inorganic electronics continue to be of large interest for sensors, bioelectronics, and security applications. Many printing techniques have been investigated, albeit often with typical minimum feature sizes in the tens of micrometer range and requiring post-processing procedures at elevated temperatures to enhance the performance of functional materials. Herein, we introduce laser printing of the semiconductor ZnO and the metals Pt and Ag by laser-induced hydrothermal synthesis and multi-photon reduction, respectively, as a facile process for fabricating printed functional electronic devices with minimum feature sizes below 1 µm. Importantly, no sintering of any sort needs to be performed after laser printing; the focused laser light itself induces the necessary local sintering during the printing process. To demonstrate the versatility of our approach, we show functional diodes, memristors, and a physically unclonable function based on a 6×6 memristor crossbar architecture. In addition, we realize functional transistors by combining laser printing and inkjet printing.
Extracellular vesicles from adult Fasciola gigantica provide clues for new vaccine targets against fascioliasis
Authors: Sheng Zhao-An , Wu Cui-Lan , Wang Dong-Ying , Zhong Shu-Hong , Yang Xi , Rao Guo-Shun , Peng Hao , Feng Shi-Wen , Li Jun , Huang Wei-Yi , Luo Hong-Lin 
Published: October 2022

Abstract
Background: Extracellular vesicles (EVs) released by helminths are important in parasite-host communication. However, little is known about the character and contents of Fasciola gigantica EVs. A better understanding of EVs released by F. gigantica will help reveal the mechanism of F. gigantica-host interaction and aid in the search for new vaccine candidates for fascioliasis control.
Methods: We used ultracentrifugation to purify two different populations of EVs (15K EVs and 100K EVs) from adult F. gigantica culture media. The morphology and size of the purified EVs were determined by transmission electron microscopy (TEM) and ZETASIZER NANO ZSP. With the aim of identifying diagnostic markers or potential vaccine candidates, proteins within the isolated 100K EVs were analyzed using mass spectrometry-based proteomics (LC-MS/MS). Then, mice were vaccinated with ESPs, 15K EVs, 100K EVs, and rFg-HSP70 combined with alum adjuvant followed by challenge infection with F. gigantica metacercariae. Fluke recovery and antibody levels were used to evaluate vaccine protection.
Results: TEM analysis and Nanoparticle tracking analysis indicated successful isolation of two subpopulations of EVs (15K EVs and 100K EVs) from adult F. gigantica culture supernatants using differential centrifugation. A total of 755 proteins were identified in the 100K EVs. Exosome biogenesis or vesicle trafficking proteins, ESCRT pathways proteins,as well as exosome markers, heat shock proteins and 14-3-3, were identified in the 100K EVs. These results indicate that the isolated 100K EVs were exosome-like vesicles. The functions of the identified proteins may be associated with immune regulation, immune evasion, and virulence. Mice immunized with F. gigantica ESP, 15K EVs, 100K EVs, and rFg-HSP70 exhibited a fluke burden reduction of 67.90%, 60.38%, 37.73%, and 56.6%, respectively, compared with the adjuvant control group. The vaccination of mice with F. gigantica 100K EVs, 15K EVs, ESP, and rFg-HSP70 induced significant production of specific IgG, IgG1, and IgG2a in sera.
Conclusion: This study suggests that proteins within the exosome-like vesicles of F. gigantica have immunomodulatory, immune evasion, and virulence functions. This knowledge may lead to new strategies for immunotherapy, vaccination, and the diagnosis of fascioliasis.
A novel self-assembled gel protects against endoscopic resection- induced colonic ulcers: a randomized clinical trial A novel gel that could promote ulcer healing
Authors: Zhou Tianyu , Xu Lei , Mao Xinli , Jin Haifeng , Cen Li , Wang Chunren , Yang Minyi , Gu Binbin , Zhang Yu , Dong Caijuan , Xin Linying , Lv Bin , Yu Chaohui , Shen Zhe 
Published: October 2022

Abstract
Background: Although associated with high en bloc and complete resection rates, endoscopic resection, which is widely performed for gastrointestinal mucosal and submucosal lesions, may cause bleeding and perforation. However, novel agents are being introduced to minimize these complications.
Methods: In this multicenter, randomized, single blind, controlled trial, participants who underwent colonic endoscopic resection across four hospitals in China were randomly assigned to the control or experimental group(1:1 ratio); the gel was used after colonic endoscopic resection in the latter. Participants were instructed to report adverse events on the first day, 14th day, and one-month post-intervention. Repeat endoscopy was performed at two-week follow-up to confirm resection site healing.
Results: Of the 92 screened participants, 73 completed the study. Each ulcer bed was successfully coated with the gel over an 87.4±13.4% area within approximately three minutes in the experimental group. The calculated healing rates were significantly higher in the experimental than in the control group (84.7±10.8% vs. 72.6±16.0%, P=0.0004). No severe relative adverse event occurred during follow-up.
Conclusions: This novel gel is a safe, effective, and convenient agent that promotes wound healing after colonic endoscopic resection. And we recommend application of this gel to protect the ulcer bed in daily clinical practice.
Trial registration This trial has been registered in Chinese Clinical Trial Registry (ChiCTR2100053692).
Comparative proteomic analysis of glomerular proteins in IgA nephropathy and IgA vasculitis with nephritis
Authors: Kaga Hajime , Matsumura Hirotoshi , Saito Ayano , Saito Masaya , Abe Fumito , Suzuki Takehiro , Dohmae Naoshi , Odaka Masafumi , Komatsuda Atsushi , Wakui Hideki , Takahashi Naoto 
Published: October 2022

Abstract
Background: IgA nephropathy (IgAN) and IgA vasculitis with nephritis (IgAVN) are related glomerular diseases characterized by marked similarities in immunological and histological findings. We herein performed a comparative proteomic analysis of glomerular proteins in IgAN and IgAVN.
Methods: We used renal biopsy specimens from 6 IgAN patients without nephrotic syndrome (NS) (IgAN-I subgroup), 6 IgAN patients with NS (IgAN-II subgroup), 6 IgAVN patients with <10% of glomeruli with crescent formation (IgAVN-I subgroup), 6 IgAVN patients with ≥20% of glomeruli with crescent formation (IgAVN-II subgroup), and 5 control cases. Proteins were extracted from laser microdissected glomeruli and analyzed using mass spectrometry. The relative abundance of proteins was then compared between groups. An immunohistochemical validation study was also performed.
Results: More than 850 proteins with high confidence were identified. A principal component analysis revealed a clear separation between IgAN and IgAVN patients and control cases. In further analyses, 546 proteins that were matched with ≥2 peptides were selected. In comparisons with the control group, higher levels (>2.6 fold) of immunoglobulins (IgA, IgG, and IgM), complements (C3, C4A, C5, and C9), complement factor H-related proteins (CFHR) 1 and 5, vitronectin, fibrinogen chains, and transforming growth factor-β inducible gene-h3 were observed in the IgAN and IgAVN subgroups, whereas lower levels (<0.3 fold) of hornerin were noted in the IgAN and IgAVN subgroups. Furthermore, the abundance of C9 and CFHR1 was significantly higher in the IgAN group than in the IgAVN group. The abundance of some podocyte-associated proteins was significantly lower in the IgAN group than in the IgAVN group. The abundance of some podocyte-associated proteins and glomerular basement membrane (GBM) proteins was also significantly lower in the IgAN-II subgroup than in the IgAN-I subgroup. Among the IgAN and IgAVN subgroups, talin 1 was not detected in the IgAN-II subgroup. This result was supported by immunohistochemical findings.
Conclusions: The present results suggest shared molecular mechanisms for glomerular injury in IgAN and IgAVN, except for enhanced glomerular complement activation and podocyte injury in IgAN. Differences in the protein abundance of podocyte-associated and GBM proteins between the IgAN subgroups may be associated with the severity of proteinuria.
Impact of prior immune checkpoint inhibitor and its tumor response on ramucirumab and docetaxel for advanced non-small cell lung cancer: a multicenter retrospective cohort study
Authors: Tanizaki Satoshi , Matsumoto Kinnosuke , Tamiya Akihiro , Taniguchi Yoshihiko , Matsuda Yoshinobu , Uchida Junji , Ueno Kiyonobu , Kawachi Hayato , Tamiya Motohiro , Yanase Takafumi , Suzuki Hidekazu , Okishio Kyoichi 
Published: October 2022

Abstract
Purpose
Ramucirumab (RAM) and docetaxel (DOC) are commonly used after first-line therapy including immune checkpoint inhibitor (ICI) for advanced non-small cell lung cancer (NSCLC). Therefore, it is important to evaluate sequential strategies of RAM and DOC following various type of treatments; however, those remain unknown. We aimed to elucidate the impact of front-line treatments including ICI, cytotoxic agent (CTx), bevacizumab (BEV), and tyrosine kinase inhibitor (TKI) on RAM and DOC efficacy.
Methods
We recruited patients with NSCLC who received RAM and DOC and compared the groups with and without prior ICI, CTx, BEV, and TKI, respectively. By tumor response to such treatments, the patients were further classified into “complete response (CR) + partial response (PR),” “stable disease.” and “progressive disease”groups, respectively. We compared RAM and DOC efficacy among these groups.
Results
237 patients were registered. In the group with prior ICI, the objective response rate and disease control rate were significantly higher than those without prior ICI (p = 0.012 and 0.028, respectively), and the median progression-free survival (PFS) was also significantly longer (p = 0.027). There were no significant differences in PFS between the groups with and without CTx, BEV, and TKI. Multivariate analysis revealed that prior ICI was an independent factor associated with better PFS. Futheremore, the prior ICI group showing CR + PR significantly prolonged PFS compared to the group without prior ICI (p = 0.013).
Conclusion
RAM and DOC efficacy may be enhanced when ICIs are administered in the prior line and especially show good tumor response.
Women's Labor Force Exits during COVID-19: Differences by Motherhood, Race, and Ethnicity
Authors: Lim Katherine , Zabek Mike 
Published: October 2022

Abstract
Women had larger increases in labor force exits than men during the COVID-19 pandemic, and increases were particularly large among women living with children. After controlling for detailed job and demographic characteristics, we find that the pandemic led to significant excess labor force exits among women living with children under age six. We also find evidence of larger increases in exits among lower-earning women living with school-aged children. The presence of children also predicted larger increases in exits during the pandemic among Latina and Black women relative to White women. Overall, we find evidence that pandemic induced disruptions to childcare, including informal care from family and friends, led to additional labor force exits by lower earning women and women of color.
JEL Numbers: J16, J70, H31, I14, I18
The bioinvasion of lionfish Pterois volitans (Linnaeus, 1758) in Brazilian waters: An urgent necessity to create strategies to contain the expansion of the species in Brazil
Authors: Hidenburgo Israel , Martins Déborah Elena Galvão , Klautau Alex Garcia Cavalleiro , Marceniuk Alexandre Pires , Santos Wagner Cesar Rosa dos , Silva Kátia Cristina de Araújo , Barbosa José Milton , Alves-Júnior Flavio de Almeida 
Published: October 2022

Abstract

The lionfish Pterois volitans (Linnaeus, 1758) is considered the most well-established fish species in non-native regions around the world, causing severe environmental damage and risks for the food chain. In Brazil, the lionfish was reported for the first time in 2014 in the State of Rio de Janeiro, and currently, the P. volitans was observed in more seven States, being its high level of occurrence in the Northern region, which contains an immense biodiversity that has not been totally cataloged yet. Based on that, this study aims to ratify the occupation and permanent residence of the lionfish in the Great Amazon Reef System (GARS), which is an alert for the urgent need for developing control strategies for this invasive species in Brazil. The new specimens were collected in the State of Amapá, between 2021 and 2022, during commercial lobster fishing, using the method of “Caçoeira”, covering between 70 and 100 meters of depth, while for the snapper was collected with the manzuá, between 78 and 102 meters of depth. In the laboratory, the individuals were measured in total length (TL) and total weight (TW). We analyzed 21 specimens of P. volitans from the GARS, the TL ranged from 21,5 to 35,6 cm with an average of 29,43 cm, while the TW ranged from 150,0 to 733,0 g, with an average of 413,8 g. These adult specimens reinforce the stabilization of the lionfish, which have probably completed reproductive cycles in the region, requiring an urgent action by environmental agencies to control the invasion of the lionfish in Brazil, becoming a future environmental impact on native biodiversity.
Effects of potassium monopersulfate on nitrification activity and bacterial community structure of sponge biocarrier biofilm in Litopenaeus vannamei aquaculture system
Authors: Luan Yazhi , Wang Yang , liu Chao , Lv Libin , Xu Ailing , Song Zhiwen 
Published: October 2022

Abstract

Effects of potassium monopersulfate (KMPS) on nitrification activity, growth performance of Litopenaeus vannamei and bacterial community structure of sponge biocarrier with pre-cultured biofilm (SBBF) were analyzed through shaking flask experiments and L. vannamei aquaculture experiment. The change of ammonia oxidation rate (AOR) and nitrite oxidation rate (NOR) of SBBF under six KMPS concentration treatments (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L, 4 mg/L and 5 mg/L) was studied, the results showed that the AOR and NOR of SBBF treated with high concentrations of KMPS (3 mg/L, 4 mg/L and 5 mg/L) were significantly lower than those of the control group (CK) (p < 0.05). However, compared with the first dosing of NH4Cl and NaNO2, the inhibition of KMPS on AOR and NOR was weakened after the second and third dosing times. The L. vannamei aquaculture experiment was set to four concentrations of KMPS (0 mg/L, 2 mg/L, 4 mg/L, 8 mg/L), the results showed that with the increase of KMPS dosage, the average and peak concentrations of NH4+-N and NO2−-N in each system significantly increased (P < 0.05). The final body weight of shrimps significantly decreased (P < 0.05), high dose (8.0 mg/L) of KMPS reduced the survival rate by 9.33% than CK. High-throughput sequencing analysis of the biofilm structure showed that the relative abundance of Nitrospirota, Nitrosomonas and Nitrococcus, which is related to nitrogen cycling, and beneficial bacteria including Firmicutes and Bacilli decreased with the addition of KMPS (p < 0.05).
Human lung carcinomas synthesize immunoregulatory glucocorticoids
Authors: Brunner Thomas , Merk Verena , Grob Leonie , Fleischmann Achim 
Published: October 2022

Abstract
The need for new options in lung cancer treatment inevitably leads back to basic research. The tumor itself and the tumor environment especially the interaction with the immune system need to be better understood to develop targeted therapies. In the context of lung cancer glucocorticoids (GC) are mainly known as a combination drug to attenuate side effects of chemotherapies. However, endogenous extra-adrenal GC have been shown to substantially regulate local immune responses within various tissues, including the lung. In this study we investigated whether primary lung tumors have maintained the capacity to synthesize GC and may thereby regulate anti-tumor immune responses. We show that several non-small cell lung carcinoma (NSCLC) and small cell lung carcinoma (SCLC) cell lines express key steroidogenic enzymes and synthesize bioactive GC under steady state conditions. We also demonstrated that tumor-derived GC can inhibit splenic T cell activation, thus demonstrating their immunoregulatory potential. Moreover, steroidogenic enzymes were detected by quantitative RT-PCR and immunohistochemistry in tissue sections of different human lung tumors, further strengthening the idea that human lung carcinomas regulate their microenvironment by releasing immunoregulatory GC.
Investigation of miR-133a, miR-637 and miR-944 genes expression and their relationship with PI3K/AKT signaling in women with breast cancer
Authors: Barartabar Zeinab , Moini Nazi , Abbasalipourkabir Roghayeh , Mesbah-Namin Seyed Alireza , Ziamajidi Nasrin 
Published: October 2022

Abstract
Background: MicroRNA (miRNA) is a regulatory molecule capable of positively or negatively regulating signaling pathways and furthermore assumes a part tumorigenesis and various aspects of cancer. The purpose of this study is to investigate the expression level of miR-133a, miR-637 and miR-944 genes in serum and tumor tissue and their relationship with the expression level of phosphatidylinositol-3-kinase (PI3K (and protein kinase-B (AKT) genes and proteins and its clinical significance in breast cancer.
Methods: The expression of miR-133a, miR-637, miR-944, PI3K and AKT genes in tumor tissues and tumor margins tissues of 40 patients with breast cancer, as well as the serum levels of miR-133a, miR-637 and miR-944 in these patients and 40 healthy groups were examined by quantitative real-time PCR (qRT-PCR). PI3K and AKT proteins expression in tumor tissue and tumor margins tissues were detected by immunohistochemistry (IHC).
Results: The expression levels of miR-133a and miR-637 in the tumor tissue and serum of patients were lower than the tumor margin tissue and serum of the healthy group, respectively. Also, the expression level of miR-944 in the tumor tissue was lower than in the tumor margin tissue, but its expression increased in the serum of cancer patients compared to the healthy group. The expression of miR-637 was correlated with tumor location, tumor size, and Her2 receptors, as well as the expression of miR-944 with tumor location and family history. PI3K and AKT mRNA and protein levels were higher in tumor tissues compared to tumor margin tissue (p<0.05).
Conclusion: The results of our study show that miR-637 has a better diagnostic value in breast cancer than miR-133a and miR-944.