bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic Implications of Birth via Cesarean Section in Prairie Vole (Microtus ochrogaster)
Authors: Starr Alexandra , Ahmed Sabreen , Partie Miranda , Kenkel William 
Published: May 2022

ABSTRACTThroughout the United States the rates of performed cesarean section (CS) have increased. The scientific community has observed an association between birth by cesarean and the offspring’s increased weight at maturity (Masukume, 2019). Studies are being conducted to better understand the relationship between cesarean delivery and offspring metabolism (Kozhimannil, 2013; Kenkel, 2020). To test this potential connection, a diet intervention study has been used to test vaginal delivery (VD) vs CS birth subject’s weight gain using a prairie vole model. Vole diets were either supplemented with a high-fat alternative mixed chow (MC) or fed standard vole chow (VC) to induce weight gain. Through this study, we collected sucrose preference, home cage, food consumption data from both birth mode groups and diet conditions. At sacrifice, we collected measures of weight, length, and adipose tissue to analyze for post-mortem body composition in adulthood of each group. CS voles gained more weight than VD voles, despite having lower food consumption and greater locomotive activity. Body composition analysis found that CS animals were longer and heavier than their VD counterparts. Additionally, CS animals were found to have a larger percent brown adipose tissue relative to body weight compared to VD counterparts. Future studies will target the variables contributing to this weight gain among CS offspring by examining factors like muscle mass, and total adiposity through advanced imaging data. Future studies will incorporate exogenous oxytocin administration to examine the impact of birth mode on body weight, metabolism, adiposity, and later life development to determine the possible mechanisms impacting the metabolic outcomes seen in this study.
Post-injury hydraulic fracturing drives fissure formation in the zebrafish basal epidermal cell layer
Authors: Kennard Andrew S. , Sathe Mugdha , Labuz Ellen C. , Prinz Christopher K. , Theriot Julie A. 
Published: May 2022

SummaryThe skin epithelium acts as the barrier between an organism’s internal and external environments. In zebrafish and other freshwater organisms, this barrier function requires withstanding a large osmotic pressure differential. Wounds breach this epithelium, causing a large disruption to the tissue microenvironment due to the mixing of isotonic interstitial fluid with the external hypotonic fresh water. Here we show that, following acute injury, the larval zebrafish epidermis undergoes a dramatic fissuring process that resembles hydraulic fracturing, driven by the influx of external fluid. The fissuring starts in the basal epidermal layer nearest to the wound, and then propagates at a constant rate through the tissue spanning over one hundred micrometers; during this process the outermost superficial epidermal layer remains intact. Fissuring is completely inhibited when larvae are wounded in an isotonic external media, suggesting that osmotic pressure gradients drive fissure. Additionally, fissuring partially depends on myosin II activity, as its inhibition reduces fissure propagation away from the wound. During and after fissuring, the basal layer forms large macropinosomes (with cross-sectional areas ranging from 1-10 µm2), presumably to clear the excess fluid. We conclude that excess external fluid entry through the wound and subsequent closure of the wound through actomyosin purse string contraction in the superficial cell layer causes fluid pressure buildup in the extracellular space of the zebrafish epidermis. This excess fluid pressure causes tissue to fissure, and eventually the fluid is cleared through macropinocytosis.
A therapeutically targetable NOTCH1-SIRT1-KAT7 axis in T-cell Leukemia
Authors: Lancho Olga , Singh Amartya , da Silva-Diz Victoria , Tottone Luca , Nunes Patricia Renck , Aleksandrova Maya , Khatun Jesminara , Luo Shirley , Zhao Caifeng , Zheng Haiyan , Chiles Eric , Zuo Zhenyu , Rocha Pedro P. , Su Xiaoyang , Khiabanian Hossein , Herranz Daniel 
Published: May 2022

AbstractT-cell Acute Lymphoblastic Leukemia (T-ALL) is a NOTCH1-driven disease in need of novel therapies. Here, we identify a NOTCH1-SIRT1-KAT7 link as a therapeutic vulnerability in T-ALL, in which SIRT1 is overexpressed downstream of a novel NOTCH1-bound enhancer. SIRT1 loss impairs leukemia generation, while SIRT1 overexpression accelerates leukemia and confers resistance to NOTCH1 inhibition in a deacetylase-dependent manner. Moreover, secondary SIRT1 loss extends survival and synergizes with NOTCH1 inhibition. Global acetyl-proteomics upon SIRT1 loss uncovered hyperacetylation of KAT7 and BRD1, subunits of a histone acetyltransferase complex targeting H4K12. Metabolic and gene expression profiling revealed a metabolic crisis together with a transcriptional signature resembling KAT7 deletion. Consistently, SIRT1 loss resulted in reduced H4K12ac, and overexpression of a non-acetylatable KAT7 mutant partly rescued SIRT1 loss-induced proliferation defects. The newly unveiled NOTCH1-SIRT1-KAT7 axis uncovers novel therapeutic targets in T-ALL and reveals a circular feedback mechanism balancing deacetylase/acetyltransferase activation with potentially broad relevance in cancer.Statement of significanceWe identified a novel axis in T-ALL whereby NOTCH1 activates SIRT1 through an enhancer region, and SIRT1 deacetylates and activates KAT7. Targeting SIRT1 shows antileukemic effects, partly mediated by KAT7 inactivation. Our results identify novel therapeutic targets and uncover a rheostat mechanism between deacetylase/acetyltransferase activities with potentially broader cancer relevance.
A global atlas of substrate specificities for the human serine/threonine kinome
Authors: Johnson Jared L. , Yaron Tomer M. , Huntsman Emily M. , Kerelsky Alexander , Song Junho , Regev Amit , Lin Ting-Yu , Liberatore Katarina , Cizin Daniel M. , Cohen Benjamin M. , Vasan Neil , Ma Yilun , Krismer Konstantin , Robles Jaylissa Torres , van de Kooij Bert , van Vlimmeren Anne E. , Andrée-Busch Nicole , Käufer Norbert , Dorovkov Maxim V. , Ryazanov Alexey G. , Takagi Yuichiro , Kastenhuber Edward R. , Goncalves Marcus D. , Elemento Olivier , Taatjes Dylan J. , Maucuer Alexandre , Yamashita Akio , Degterev Alexei , Linding Rune , Blenis John , Hornbeck Peter V. , Turk Benjamin E. , Yaffe Michael B. , Cantley Lewis C. 
Published: May 2022

ABSTRACTProtein phosphorylation is one of the most widespread post-translational modifications in biology. With the advent of mass spectrometry-based phosphoproteomics, more than 200,000 sites of serine and threonine phosphorylation have been reported, of which several thousand have been associated with human diseases and biological processes. For the vast majority of phosphorylation events, it is not yet known which of the more than 300 protein Ser/Thr kinases encoded in the human genome is responsible. Here, we utilize synthetic peptide libraries to profile the substrate sequence specificity of nearly every functional human Ser/Thr kinase. Viewed in its entirety, the substrate specificity of the kinome was substantially more diverse than expected and was driven extensively by negative selectivity. Our kinome-wide dataset was used to computationally annotate and identify the most likely protein kinases for every reported phosphorylation site in the human Ser/Thr phosphoproteome. For the small minority of phosphosites where the protein kinases involved have been previously identified, our predictions were in excellent agreement. When this approach was applied to examine the signaling response of tissues and cell lines to hormones, growth factors, targeted inhibitors, and environmental or genetic perturbations, it revealed unexpected insights into pathway complexity and compensation. Overall, these studies reveal the full extent of substrate specificity of the human Ser/Thr kinome, illuminate cellular signaling responses, and provide a rich resource to link unannotated phosphorylation events to biological pathways.
Immunoregulatory subtype of dermal lymphatic endothelial cells at capillary terminals drives lymphatic malformations
Authors: Petkova Milena , Kraft Marle , Stritt Simon , Martinez-Corral Ines , Ortsäter Henrik , Sun Ying , Vanlandewijck Michael , Jakic Bojana , Baselga Eulàlia , Castillo Sandra D. , Graupera Mariona , Betsholtz Christer , Mäkinen Taija 
Published: May 2022

AbstractVascular malformations are congenital, chronically debilitating diseases. Somatic oncogenic mutations in PIK3CA, encoding p110α-PI3K, specifically cause venous and lymphatic malformations (LM), yet the basis of vessel type-restricted disease manifestation is unknown. Here we report endothelial subtype-specific responses to the common causative Pik3caH1047R mutation, and reveal a new immunoregulatory subtype of dermal lymphatic capillary endothelial cells (iLECs) as a driver of LM pathology. Mouse model of Pik3caH1047R-driven vascular malformations showed that cell proliferation was a common early response of venous and lymphatic ECs to oncogenic Pik3ca, but sustained selectively in LECs of advanced lesions. Lymphatic overgrowth was associated with increased pro-inflammatory cytokine levels and pro-lymphangiogenic myeloid cell infiltrate. Single-cell transcriptomics revealed a new LEC subtype at capillary terminals, characterized by the expression of immunoregulatory genes. Selective expansion and activation of iLECs in the Pik3caH1047R mice was evidenced by proliferation and upregulation of pro-inflammatory genes. Importantly, macrophage depletion or anti-inflammatory COX-2 inhibition limited Pik3caH1047R-driven lymphangiogenesis. This provides a therapeutic target for LM and suggests a paracrine crosstalk in which LEC-autonomous oncogenic Pik3ca signaling induces immune activation that in turn sustains pathological lymphangiogenesis. Identification of iLECs indicates that peripheral lymphatic vessels not only respond to inflammation but also actively orchestrate the immune response.
Managerial coaching behavior and subordinates’ learning effects in cross-cultural context: a moderated mediation study
Authors: Niu Xiongying , Zhang Baofang , Simasiku Mulele , Zhang Rui 
Published: May 2022

Purpose
This study aims to explore the effect of expatriate supervisors’ managerial coaching behavior on local subordinates’ learning effects through the mediating role of subordinates’ thriving at work under the boundary condition of expatriate supervisors’ cultural intelligence.

Design/methodology/approach
This study collected the data form 230 Zambian subordinates and their immediate expatriate supervisors working in the Chinese company in Zambia. Regression analyses and bootstrapping analyses were used to test the authors’ hypothesis.

Findings
The results indicated that expatriate supervisors’ managerial coaching behavior was positively related to local subordinates’ learning effects. In addition, the study also found that local subordinates’ thriving at work mediated the linkage between managerial coaching behavior and learning effects. And expatriate supervisors’ cultural intelligence moderated the indirect relationship between managerial coaching behavior and learning effects via thriving at work, such that the indirect effect was stronger for expatriate supervisors with high rather than low cultural intelligence.

Originality/value
This study contributes to a better understanding of how expatriate supervisors’ managerial coaching behavior influences local subordinates’ learning effects by investigating the mediating effect of thriving at work on the managerial coaching behavior–learning effects link. In addition, the study deepens the understanding of the boundary condition of the associations between managerial coaching behavior and subordinates’ learning effects in a cross-cultural context by investigating the moderating effect of expatriate supervisors’ cultural intelligence.
Zooming into paternalistic leadership: evidence from high power distance culture
Authors: Islam Talat , Sharif Saleha , Ali Hafiz Fawad , Jamil Saqib 
Published: May 2022

PurposeNurses' turnover intention has become a major issue in developing countries with high power distance cultures. Therefore, the authors attempt to investigate how turnover intention among nurses' can be reduced through paternalistic leadership (PL). The authors further investigate the mediating role of job satisfaction between the associations of benevolent, moral and authoritarian dimensions of PL with turnover intention. Finally, the authors examined perceived organizational support (POS) as a conditional variable between job satisfaction and turnover intention.Design/methodology/approachThe authors collected data from 374 nurses working in public and private hospitals of high power distance culture using a questionnaire-based survey on convenience basis.FindingsStructural equation modeling confirms that benevolent and moral dimensions of PL positively affect nurses' job satisfaction which helps them reduce their turnover intention. While the authoritarian dimension of PL negatively affects job satisfaction to further enhance their turnover intention. In addition, the authors noted POS as a conditional variable to trigger the negative effect of job satisfaction on turnover intention.Research limitations/implicationsThe authors used a cross-sectional design to collect responses and ensured the absence of common method variance through Harman's Single factor test.Originality/valueThis study identified the mechanism (job satisfaction and POS) through which benevolent, moral and authoritative dimensions of PL predict turnover intention among nurses working in high power distance culture.
Lessons learned from historical development and modern practice of marketplaces – focus on the Serbian capital city
Authors: Petrović Marko D. , Ledesma Edna , Štetić Snežana , Trišić Igor , Radovanović Milan M. 
Published: May 2022

PurposeThe starting premise of the case study is to describe the ongoing interventions and experiences within the observed public marketplaces' organization. The objective of the research is to examine specified aspects of social and economic perspectives and the role of marketplaces in changing the local surroundings and economy of the Serbian capital and its largest city – Belgrade.Design/methodology/approachMultiple methods of analysis, such as archival investigation, participants' observation and semi-structured interviews were conducted. To inspect the research questions, the case implemented qualitative research that sampled 21 employees in the examined public company which manages all the analyzed urban marketplaces.FindingsThis case study will describe the city-sponsored organization, the Public Utility Company (PUC), that sustains marketplaces and the implications of that city sponsorship. Additionally, the outcomes describe the social and economic impact of marketplaces in placemaking around the region.Practical implicationsThe most imperative implications of the manuscript are twofold: (1) the research results have shown that the potential of the city marketplaces can be increased through the support of the PUC and the city government; (2) as one of the first empirical projects about the social organization of the marketplaces in this part of Europe, the findings provide an overview of the contemporary market processes, and market outcomes. This study can affect other future research to explore similar aspects of the markets' organization.Social implicationsThis research can encourage comparable future examinations to explore other components of the market, varying in the regional diversities on one hand, and the manifold prospects for the community development with fewer benefits, on the other.Originality/valueThe study analyzes all the local markets in the selected urban area. This is the first empirical research on the social perspective and the role of marketplaces in the process of changing the modern society and economy in Belgrade. Moreover, it may contribute to future analysis in the field of social perspective and economic directions in future strategies of city planning.
Transformation of low‐molecular‐weight organic acids by microbial endoliths in subsurface mafic and ultramafic igneous rock
Authors: Fones Elizabeth M. , Templeton Alexis S. , Mogk David W. , Boyd Eric S. 
Published: May 2022

SummaryA growing body of work indicates that continental subsurface rocks host a substantial portion of the Earth's biosphere. However, the activities of microbial cells inhabiting pore spaces and microfractures in subsurface rocks remain underexplored. Here, we develop and optimize microcosm assays to detect organic acid transformation activities of cells residing in mafic to ultramafic igneous rocks. Application of this assay to gabbro core from the Stillwater Mine, Montana, USA, revealed maximal methane production from acetate at temperatures approximating that of the mine. Controls show that these activities are not due to contamination introduced during drilling, exhumation, or laboratory processing of the core. The assay was then applied to rocks cored from the Samail Ophiolite, Oman, which is undergoing low‐temperature serpentinization. Production of (i) carbon dioxide from acetate and formate and (ii) methane from formate were detected in a dunite/harzburgite rock core interfacing pH 9.6 waters, and estimates of microbial activities were up to three orders of magnitude higher in the rock core pore space than in corresponding waters. The detection of endolithic microbial activities in igneous rocks has implications for life detection on other planetary bodies where similar rock types prevail, such as Mars, Europa and Enceladus.
Adaptation or inflexibility? Niche party responsiveness to policy competition, with evidence from regionalist parties
Authors: MEGUID BONNIE M. 
Published: May 2022

AbstractDespite a growing literature on niche parties, little is known about whether and how these parties are responsive to policy competition. Drawing upon data on regionalist parties’ programmatic strategies across Western Europe from 1971 to 2009, I find that these niche parties are more ideologically flexible than previously posited when facing governmental policy appeasement. While they do not shift to a more extreme issue position following increases in a region's decentralization level, regionalist parties do broaden their issue agenda. Issue diversification, however, is limited to those parties whose goals are being met; dissatisfied secessionist parties do not expand their issue emphases after decentralization reforms short of independence. Supported by emerging evidence of similar agenda diversification among appeased green and radical right parties, these analyses further our understanding of niche parties as strategic actors and offer new explanations for variation in the effectiveness of mainstream party tactics towards them.
Mister Chips goes to Brussels: On the Pros and Cons of a Semiconductor Policy in theEU
Authors: Hancké Bob , Garcia Calvo Angela 
Published: May 2022

AbstractIs making semiconductors in Europe a good idea? With the introduction of the European Chips Act in early 2022, this is no longer an academic question. In this article we evaluate these plans in light of a wider—but remarkably one‐sided—debate on industrial policy in advanced capitalist economies. Instead of focusing on policymakers, as most students of industrial policy have done, we draw attention to two first‐order conditions that determine the chances of policy success: the existence of (proto‐)competitive incumbents, and the alignment of the policy objectives with the prevailing institutional framework. We rely on that framework to evaluate the successes and failures in European aerospace, German biotech, and French computers, before using these insights to evaluate the European Chips Act. Investing in manufacturing of mature chips is, in that light, not a good idea. We suggest, instead, that the EU should concentrate on market segments that leverage its high‐skill workplaces and world‐class research system, and in which Europe has either already developed a comparative advantage or will not find itself at an initial disadvantage.
The relationship between smokeless tobacco (snus) and anxiety and depression among adults and elderly people. A comparison to smoking in the Tromsø Study
Authors: Bondø Pia , Høye Anne , Løchen Maja‐Lisa , Bramness Jørgen G. 
Published: May 2022

AbstractAimsTo (i) define the characteristics of snus users compared with non‐users and smokers and (ii) define the relationship between snus use and self‐reported anxiety and depression and compare it with the relation between smoking and anxiety and depression.Design, setting and participantsA cross‐sectional study based on data from the Norwegian population‐based survey, the Tromsø Study (2015–16). A total of 32 591 people aged 40 years and older in the municipality of Tromsø were invited to attend. There were 21 083 respondents, giving a 65% attendance rate.MeasurementsTobacco use was assessed by current and previous use of snus or cigarettes. Symptoms of anxiety and depression were measured using the Hospital Anxiety and Depression Scale (HADS).FindingsSnus users were more often male, were younger and had higher income and higher alcohol consumption than smokers and non‐users of snus. In a linear regression analysis, we found no significant association between current snus use and anxiety and depression after adjusting for background variables. However, there was a positive association between previous snus use and anxiety among males [adjusted beta = 0.258; 99% confidence interval (CI) = 0.023–0.492]. Regarding anxiety, the adjusted beta‐coefficients for current smoking were 0.425 (99% CI = 0.184–0.666) for females and 0.303 (99% CI = 0.084–0.522) for males. Concerning depression, the adjusted beta coefficients for current smoking were 0.569 (99% CI = 0.358–0.780) for females and 0.281 (99% CI = 0.060–0.503) for males.ConclusionsIn Norway, current snus users differ from current smokers by having a higher socio‐economic status and no detectable association with anxiety and depression. This suggests that the relationship between tobacco use and anxiety and depression is associated with the administration method.
On export duration puzzles
Authors: Zongo Wendkouni Jean‐Baptiste , Larue Bruno , Gaigné Carl 
Published: May 2022

AbstractWe investigate two puzzles in the export duration literature. The first puzzle has to do with the frequent entries and exits of firms in export markets, which are at odds with the large fixed export costs in such markets. We introduce convex production technologies in a trade model to show how variable marginal costs create direct linkages between export markets. As fixed export costs vary across destinations, more productive firms need not necessarily export to more destinations. Cost convexity implies that the probability of supplying a given export market is adversely affected by positive export shocks in other markets. This is supported by our empirical analysis of bilateral flows for over 200 agri‐food products to 176 destinations originating from six large exporting countries. The second puzzle has to do with the paradoxical effect of tariffs reported in empirical export duration studies. When endogeneity is addressed, tariffs increase the probability of an export failure.