bluuebunny/crossref_metadata_2025_split huggingface dataset:
DEVELOPMENT OF INTERACTIVE DIGITAL MODULES ON FLAT BUILDING SPACE MATERIALS FOR CLASS IV STUDENTS
Authors: F.T.H Dina Valentina , Juniarso Triman , Yustitia Via 
Published: October 2022

Interactive digital modules for broad flat wake materials for grade IV students are motivated by, the variety of use of teaching materials, especially in mathematics learning in Kepuh Kiriman Elementary School 1. Interactive digital modules can be used to learn independently because students get feedback (scores) directly. The purpose of this research is to describe the process of developing interactive digital modules on broad flat wake materials for grade IV students and know their feasibility. This research uses a type of development research or commonly known as Research and Development (R&D). Using the ADDIE model (Analysis, Design, Development, Implementation, Evaluation). However, it is limited to the Analysis, Design and Development stage.Â Data collection methods use questionnaires and documentation. Development research instruments used to assess the feasibility of interaktif digital modules are in the form of product feasibility questionnaires. Data analysis techniques use descriptive data analysis techniques for product processing and quantitative analysis to determine product feasibility. The results of this research are products of development results through three stages, namely Analysis (needs analysis, student analysis, content analysis / material), Design (the stage used to create an interactive digital module content framework) and Development (in the form of product development results.
Globalization Processes and Migration Mechanisms
Authors: Organiściak-Krzykowska Anna , Sieciński Kacper 
Published: October 2022

The aim of this article is to analyse and assess the impact of globalisation processes in the context of migration mechanisms. The analysis is based on statistical data from International Organization for Migration (IOM), United Nations Department of Economic and Social Affairs (UNDESA), United Nations High Commissioner for Refugees (UNHCR) and Eurostat. Essentially, globalization consists of ongoing processes involving the flow of goods, information, capital, human resources and the development of transport and communication on an international scale. With advancing globalization processes, procedures applicable to the movement of human capital are simplified, and, at the same time, international migration intensifies. With respect to migration mechanisms, international agreements play an important role, as they establish rules for the movement of migrants across the borders of the state parties to particular agreements. The continually unfolding globalization processes in the 21st century indicate an increasingly evident trend whereby people from poor countries migrate to more affluent countries. Due to the high correlation between migration and socio-economic development, the issue of human capital transfer has become a matter of interest for international economic institutions, particularly where developing countries are concerned. Consequently, greater attention is focused on migration processes and their impact on the social and economic circumstances in particular countries.
Is Fieldwork losing its grace? Encountering Western and Indian Experience
Authors: Midya Dipak Kumar 
Published: October 2022

Anthropology is primarily a field science as the field provides the basic platform for critical scrutiny of ideas and theories of the discipline. Fieldwork is also an extremely indispensable tool to understand the culture of the ‘observed’. Magnificent outcomes of fieldwork in the hands of Malinowski, Mead, Evans-Pritchard, Barth, Firth, Bohannan, Levi-Strauss, Powdermaker, and others have been instrumental in the development of various discourses in anthropology and allied disciplines. Contributions of Indian anthropologists in field studies are also no less significant as these reflect renewed interest in empiricism and the reflexive understanding of the culture of the ‘others’. But nowadays fieldwork is getting less importance in western as well as in Indian academics. In this article, I have tried to critically examine various issues in connection with the present days’ fieldwork enterprise which is getting a diminishing importance.
BREAKING WAVES HEIGHT FORECASTING AT THE SHORELINE OF PANCENG SUB-DISTRICT GRESIK DISTRICT
Authors: Loka Syah Putra Aira Loka , Iwan Wahjudijanto  , Minarni Nur Trilita  
Published: October 2022

Shoreline is a line where the edge of the land and the sea met. Various amount of shore construction was built around the shoreline. The safety of surrounding community near the shoreline should be considered before the government planning on near shore and shoreline facility. Therefore, breaking waves height become important because of the impact that it brought to the shoreline, such as the destructive energy that breaking waves generates which would wipe anything that existed alongshore.
Using the data collected from BMKG (Meteorology, Climatology, and Geophysics Council), maximum wind stress at the Panceng Sub-District was 17,2 m/s. Average effective fetch at Panceng Sub-District was 331,68 km with 5 m significant wave height and 11,2 s period. Breaking wave height at Panceng Sub-District is 5,5 m.
Stable reconstructed human gingiva–microbe interaction model: Differential response to commensals and pathogens
Authors: Zhang Yan , Shang Lin , Roffel Sanne , Krom Bastiaan P. , Gibbs Susan , Deng Dongmei 
Published: October 2022

BackgroundTo investigate human oral health and disease, models are required which represent the interactions between the oral mucosa and microbiome. Our aim was to develop an organotypic model which maintains viability of both host and microbes for an extended period of time.MethodsReconstructed Human Gingiva (RHG) were cultured air-lifted with or without penicillin-streptomycin (PS) and topically exposed to Streptococcus gordonii (commensal) or Aggregatibacter actinomycetemcomitans (pathogen) for 72 hours in agar. RHG histology, viability and cytokines (ELISA), and bacterial viability (colony forming units) and location (FISH) were assessed.ResultsThe low concentration of topically applied agar did not influence RHG viability. Topically applied bacteria in agar remained localized and viable for 72 hours and did not spill over to infect RHG culture medium. PS in RHG culture medium killed topically applied bacteria. Co-culture with living bacteria did not influence RHG viability (Ki67 expression, MTT assay) or histology (epithelium differentiation, Keratin10 expression). RHG exposed to S. gordonii (with or without PS) did not influence low level of IL-6, IL-8, CCL2, CCL5, CCL20 or CXCL1 secretion. However, all cytokines increased (except CCL2) when RHG were co-cultured with A. actinomycetemcomitans. The effect was significantly more in the presence of living, rather than dead, A. actinomycetemcomitans. Both bacteria resulted in increased expression of RHG antimicrobial peptides (AMPs) Elafin and HBD-2, with S. gordonii exposure resulting in the most Elafin secretion.ConclusionThis technical advance enables living human oral host–microbe interactions to be investigated during a 72-hour period and shows differences in innate immunology triggered by S. gordonii and A. actinomycetemcomitans.
Dose-dependent stimulation of human follicular steroidogenesis by a novel rhCG during ovarian stimulation with fixed rFSH dosing
Authors: Bøtkjær Jane Alrø , Kristensen Stine Gry , Olesen Hanna Ørnes , Larsson Per , Mannaerts Bernadette , Andersen Claus Yding 
Published: October 2022

BackgroundChoriogonadotropin (CG) beta (FE 999302), a novel recombinant human (h)CG produced by a human cell line, has a longer half-life and higher potency than CG alfa produced by a Chinese hamster ovary cell line. hCG augments steroid production, but the extent of which CG beta treatment during ovarian stimulation (OS) increases steroidogenesis is unknown.ObjectiveTo explore how increasing doses of CG beta during OS augment follicular steroidogenesis and change gene expression in cumulus cells.Study designThis study is part of a randomized, double-blind, placebo-controlled trial to investigate the efficacy and safety of CG beta plus recombinant follicle-stimulating hormone (rFSH) in women undergoing OS during a long gonadotrophin-releasing hormone agonist protocol. The study primary endpoint was intrafollicular steroid concentrations after CG beta administration. Secondary outcomes were gene expression of FSHR, LHR, CYP19a1, and androgen receptor (AR).Participants/methods619 women with anti-Müllerian hormone levels 5–35 pmol/L were randomized to receive placebo or 1, 2, 4, 8, or 12 µg/day CG beta from Day 1 of OS plus rFSH. Follicular fluid (FF) (n=558), granulosa (n=498) and cumulus cells (n=368) were collected at oocyte retrieval. Steroid FF hormones were measured using enzyme-linked immunosorbent assays, gene expression was analyzed in cumulus cells by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and single nucleotide polymorphism (SNP) analysis was performed in granulosa cells.Results17-OH-progesterone, androstenedione, testosterone, and estradiol concentrations significantly increased in a CG-beta dose-dependent manner during OS (p&lt;0.0001), reaching up to 10 times higher values in the highest dose group versus placebo. There was no difference between CG beta dose groups and placebo for progesterone. Expression levels of CYP19a1 increased significantly in the highest dose group of CG beta (p=0.0325) but levels of FSHR, LHR and AR were not affected by CG beta administration. There were no differences between the FSHR(307) or LHR(312) SNP genotypes for dose-dependent effects of CG beta in relation to number of oocytes, intrafollicular steroid hormone levels, or gene expression levels.ConclusionsThese results reflect the importance of the combined effect of FSH and hCG/LH during OS on granulosa cell activity, follicle health and potentially oocyte quality.Trial Registration number2017-003810-13 (EudraCT Number).Trial Registration date21 May 2018.Date of first patient’s enrolment13 June 2018. Presented at the 38th Annual Meeting of the European Society of Human Reproduction and Embryology, P-567, 2022.
Triglyceride−glucose index in the prediction of major adverse cardiovascular events in patients with type 2 diabetes mellitus after coronary artery bypass surgery: A retrospective cohort study
Authors: Zhang He , Chong Hoshun , Li Zeshi , Li Kai , Zhang Bomin , Xue Yunxing , Wang Dongjin 
Published: October 2022

BackgroundInsulin resistance (IR) is a significant risk factor for cardiometabolic diseases and a defining feature of type 2 diabetes mellitus (T2DM). This study aimed to examine the potential value of triglyceride-glucose (TyG) index as a predictor of prognosis in coronary heart disease (CHD) patients with T2DM after coronary artery bypass grafting (CABG) surgery and to facilitate the identification of those at high risk of major adverse cardiovascular events (MACEs) for closer monitoring or possible early intervention.MethodsThis study enrolled 386 T2DM patients who underwent CABG surgery at Nanjing Drum Tower Hospital. Patients were separated into two groups according to the median preoperative TyG Index. The Kaplan-Meier plot was used to compare the rate of MACEs-free survival in T2DM patients after CABG. The independent risk factors for the occurrence of MACEs were investigated using multivariate analysis. Nomogram was used to depict the predictive model.ResultsSignificantly more MACEs occurred in individuals with higher medians of the TyG index (65 (33.7%) vs. 39 (20.2%), p=0.003). TyG index [hazard ratio (HR) 12.926], LVEF [hazard ratio (HR) 0.916], and NYHA functional class III/IV [hazard ratio (HR) 4.331] were identified as independent predictors of MACEs incidence in post-CABG T2DM patients by multivariate analysis. The area under the curve (AUC) for predicting MACEs using the TyG index was 0.89 at five years. Combining the TyG index, LVEF, and NYHA functional class III/IV to build a novel risk assessment model for postoperative MACEs, the AUC climbed to 0.93 at five years. With AUCs, the nomogram comprised of the TyG index, LVEF, and NYHA functional class III/IV demonstrated strong specificity in the training and test sets.ConclusionsThe incidence of MACEs is high among post-CABG T2DM patients with a high TyG index. TyG index improves the diagnostic accuracy of MACEs, especially at long-term follow-up. A high TyG index may serve as an early warning signal for individuals to undertake lifestyle adjustments that can reduce the progression or incidence of MACEs.
Response to targeted radionuclide therapy with [131I]MIBG AND [177Lu]Lu-DOTA-TATE according to adrenal vs. extra-adrenal primary location in metastatic paragangliomas and pheochromocytomas: A systematic review
Authors: Prado-Wohlwend Stefan , del Olmo-García María Isabel , Bello-Arques Pilar , Merino-Torres Juan Francisco 
Published: October 2022

PurposeTargeted radionuclide therapy (TRT) with [131I]MIBG and [177Lu]Lu-DOTA-TATE is an alternative treatment to the classic schemes in slow progressive metastatic/inoperable paraganglioma (PGL) and pheochromocytoma (PHEO). There is no consensus on which treatment to administer and/or the best sequence in patients who are candidates for both therapies. To clarify these questions, this systematic review assesses the prognostic value of [131I]MIBG and [177Lu]Lu-DOTA-TATE (PRRT-Lu) treatments in terms of progression-free survival (PFS) both globally and considering the primary location.MethodsThis review was developed according to the PRISMA Statement with 27 final studies (608 patients). Patient characteristics, treatment procedure, and follow-up criteria were evaluated. In addition, a Bayesian linear regression model weighted according to its sample size and an alternative model, which also included an interaction between the treatment and the proportion of PHEOs, were carried out, adjusted by a Student’s t distribution.ResultsIn linear regression models, [131I]MIBG overall PFS was, on average, 10 months lower when compared with PRRT-Lu. When considering the interaction between treatment responses and the proportion of PHEOs, PRRT-Lu showed remarkably better results in adrenal location. The PFS of PRRT-Lu was longer when the ratio of PHEOs increased, with a decrease in [131I]MIBG PFS by 1.9 months for each 10% increase in the proportion of PHEOs in the sample.ConclusionMethodology, procedure, and PFS from the different studies are quite heterogeneous. PRRT-Lu showed better results globally and specifically in PHEOs. This fact opens the window to prospective trials comparing or sequencing [131I]MIBG and PRRT-Lu.
Implications of metabolic health status and obesity on the risk of kidney cancer: A nationwide population-based cohort study
Authors: Cho Yun Kyung , Kim Hwi Seung , Park Joong-Yeol , Lee Woo Je , Kim Ye-Jee , Jung Chang Hee 
Published: October 2022

PurposeThis study evaluated the association between metabolic health status and incident kidney cancer among obese participants.Materials and methodsA total of 514,866 individuals were included from the Korean National Health Insurance Service-National Health Screening Cohort. Changes in metabolic health status and obesity from the baseline examination in 2009–2010 to the next biannual examination in 2011–2012 were determined. Based on the status change, obese participants were divided into four groups: stable metabolically healthy obesity, metabolically healthy obesity to metabolically unhealthy obesity, metabolically unhealthy obesity to metabolically healthy obesity, and stable metabolically unhealthy obesity.ResultsThe stable metabolically healthy obesity phenotype did not confer an increased risk of incident kidney cancer, compared to the stable metabolically healthy non-obese group. In contrast, the metabolically healthy obesity to metabolically unhealthy obesity group had a significantly higher risk of incident kidney cancer than the stable metabolically healthy non-obese group. Among patients with metabolically unhealthy obesity at baseline, those who transitioned to the metabolically healthy obese group had no increased risk of kidney cancer, whereas those who remained in metabolically unhealthy obesity status had a higher risk of incident kidney cancer than the stable metabolically healthy non-obese group. The transition or maintenance of metabolic health was a decisive factor for kidney cancer in obese patients.ConclusionsMaintaining or restoring metabolic health should be stressed upon in obese patients to reduce the risk of kidney cancer.
Hsa_circ_0040809 and hsa_circ_0000467 promote colorectal cancer cells progression and construction of a circRNA-miRNA-mRNA network
Authors: Liu Jingfu , Chen Shan , Li Zhen , Teng Wenhao , Ye Xianren 
Published: October 2022

Objective: Circular RNAs (circRNAs) have been demonstrated to be closely involved in colorectal cancer (CRC) pathogenesis and metastasis. More potential biomarkers are needed to be searched for colorectal cancer (CRC) diagnosis and treatment. The objective of this study is to seek differentially expressed circRNAs (DEcircRNAs), test their roles in CRC and construct a potential competing endogenous RNA (ceRNA) network.Methods: CircRNA microarrays were obtained from Gene Expression Omnibus, and differential expression was analyzed by R software. The relative expressions of DEcircRNAs were confirmed in CRC tissues and cell lines by qRT-PCR. MTs and Transwell experiments were performed for detecting the roles of circRNAs on CRC cell proliferation and migration, respectively. Targeted miRNAs of circRNAs and targeted mRNAs of miRNAs were predicted and screened by bioinformatics methods. A ceRNA network of DEcircRNAs was constructed by Cytoscape. To further verify the potential ceRNA network, the expressions of miRNAs and mRNAs in knockdown of DEcircRNAs CRC cells were detected by qRT-PCR.Results: Two DEcircRNAs (hsa_circ_0040809 and hsa_circ_0000467) were identified and validated in CRC tissues and cell lines. The results of MTs and Transwell experiments showed that hsa_circ_0040809 and hsa_circ_0000467 promoted CRC proliferation and migration. Bioinformatics analysis screened 3 miRNAs (miR-326, miR-330-5p, and miR-330-3p) and 2 mRNAs (FADS1 and RUNX1), and a ceRNA network was constructed. In knockdown of hsa_circ_0040809 HCT-116 cells, the expression of miR-330-3p was significantly upregulated, while RUNX1 was significantly downregulated. In knockdown of hsa_circ_0000467 HCT-116 cells, the expressions of miR-326 and miR-330-3p were upregulated, while FADS1was downregulated.Conclusion: We found that hsa_circ_0040809 and hsa_circ_0000467 were upregulated in CRC tissues and cell lines, and promoted CRC cell progression. A circRNA-miRNA-mRNA network based on hsa_circ_0040809 and hsa_circ_0000467 was constructed.
Immune-associated pivotal biomarkers identification and competing endogenous RNA network construction in post-operative atrial fibrillation by comprehensive bioinformatics and machine learning strategies
Authors: Zhou Yufei , Wu Qianyun , Ni Gehui , Hong Yulu , Xiao Shengjue , Liu Chunjiang , Yu Zongliang 
Published: October 2022

BackgroundAtrial fibrillation (AF) is the most common arrhythmia. Previous studies mainly focused on identifying potential diagnostic biomarkers and treatment strategies for AF, while few studies concentrated on post-operative AF (POAF), particularly using bioinformatics analysis and machine learning algorithms. Therefore, our study aimed to identify immune-associated genes and provide the competing endogenous RNA (ceRNA) network for POAF.MethodsThree GSE datasets were downloaded from the GEO database, and we used a variety of bioinformatics strategies and machine learning algorithms to discover candidate hub genes. These techniques included identifying differentially expressed genes (DEGs) and circRNAs (DECs), building protein-protein interaction networks, selecting common genes, and filtering candidate hub genes via three machine learning algorithms. To assess the diagnostic value, we then created the nomogram and receiver operating curve (ROC). MiRNAs targeting DEGs and DECs were predicted using five tools and the competing endogenous RNA (ceRNA) network was built. Moreover, we performed the immune cell infiltration analysis to better elucidate the regulation of immune cells in POAF.ResultsWe identified 234 DEGs (82 up-regulated and 152 down-regulated) of POAF via Limma, 75 node genes were visualized via PPI network, which were mainly enriched in immune regulation. 15 common genes were selected using three CytoHubba algorithms. Following machine learning selection, the nomogram was created based on the four candidate hub genes. The area under curve (AUC) of the nomogram and individual gene were all over 0.75, showing the ideal diagnostic value. The dysregulation of macrophages may be critical in POAF pathogenesis. A novel circ_0007738 was discovered in POAF and the ceRNA network was eventually built.ConclusionWe identified four immune-associated candidate hub genes (C1QA, C1R, MET, and SDC4) for POAF diagnosis through the creation of a nomogram and evaluation of its diagnostic value. The modulation of macrophages and the ceRNA network may represent further therapy methods.
Impaired intratumoral natural killer cell function in head and neck carcinoma
Authors: Mele Dalila , Pessino Greta , Trisolini Giuseppe , Luchena Alberto , Benazzo Marco , Morbini Patrizia , Mantovani Stefania , Oliviero Barbara , Mondelli Mario U. , Varchetta Stefania 
Published: October 2022

Natural killer (NK) cells are emerging as unique players in the immune response against cancer; however, only limited data are available on tumor infiltrating NK cells in head and neck squamous cell carcinoma (HNSCC), one of the most common cancer. Occurrence of HNSCC is closely related to the immune microenvironment, and immunotherapy is increasingly being applied to this setting. However, the limited success of this type of treatment in this tumor calls for further investigation in the field.Surgical HNSSC specimens of 32 consecutive patients were mechanically and enzymatically dissociated. Tumor cells were separated from infiltrating cells by short centrifugation and infiltrating NK cells were phenotypically and functionally characterized by multiple antibody staining and flow cytometry. Tumor infiltrating NK cells in HNSCC showed a peculiar phenotype predominantly characterized by increased NKG2A and reduced Siglec-7, NKG2D, NKp30 and CD16 expression. This phenotype was associated with a decreased ability to perform antibody-dependent cellular cytotoxicity (ADCC). However, NK, CD4 and CD8 shared an increment of glucocorticoid-induced tumor necrosis factor-related (GITR) costimulatory receptor which could be exploited for immunotherapy with agonistic anti-GITR antibodies combined with checkpoint inhibitors.