bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does the transition to renewable energy increase inflation in European countries?
Authors: ARSLAN Ünal , Yıldız Tuba 
Published: March 2022

Abstract
In European countries, because of the expensiveness of the technology required for the use of renewable energy sources, it can cause inflation by constantly increasing prices due to cost increases in the short term. However, in the long term, costs can be reduced by providing the necessary technological infrastructure, saving energy thanks to this technological development, eliminating harmful effects on the environment. In this way, renewable energy sources have a long-term effect on inflation. This study aims to examine the long-term relationship between renewable energy and inflation in European Union countries. It is thought that this study will contribute to the literature, since there are no studies that examine the relationship between renewable energy and inflation, among the national and international studies, which deal with the European Union countries and examine the PMG and CCE co-integration analyses. According to the results of the PMG cointegration analysis, while there is no significant relationship between inflation and renewable energy in the short term, renewable energy affects inflation negatively in the long term. According to the results of the CCE cointegration analysis, in the long run, renewable energy affects inflation negatively in Sweden, the United States, and Luxembourg.
Cadmium Affect Root Growth in Japonica Rice (Oryza sativa L.) Negatively Interacting with JA at Bud Stage
Authors: Wu Bohan , Zeng Zifan , Wu Xiangyang , Li Yingying , Wang Fangquan , Yang Jie , Li Xia 
Published: March 2022

Abstract
To explore the role of jasmonic acid in seed germination of rice under Cd stress, the growth indices of JJ818 and NJ9108 under the bud stage were measured for identifying study the molecular mechanism of variety difference in cadmium tolerance. The tolerant variety JJ818 and the sensitive variety NJ9108 were treated under 150 µmol·L− 1 Cd concentration, methyl jasmonate (MeJA), and Ibuprofen (IBU) alone or in combination. The germination rate, root length, bud length, soluble protein content, soluble sugar content, and sugar components, α-amylase activity, and its related gene expression were measured. The results showed that the root length of the two rice materials were significantly inhibited during germination under the Cd treatment, however, the germination rate and shoot length had little affected. MeJA decreased the germination rate, root length and shoot length, and the contents of soluble sugar and sugar components and α-amylase activity as well. However, IBU treatment showed the opposite trend. Cd treatment also decreased the expression of the αAmy and RL1, and the accompanying changes of up-regulated the gene expression of JIOsPR10, OsLOX1, and OsLOX4 related to synthesizing jasmonic acid(JA). The results showed that JA negatively regulates the cadmium of rice and the genes related to the synthesis and response of JA were related to the variety difference in cadmium tolerance at the bud stage. Compared with the NJ9108, JJ818 was insensitive to jasmonic acid.
Microenvironment-related gene IGLL5 has potential to predict the prognosis of clear cell renal cell carcinoma
Authors: Bu Ning , Dan Weichao , Li Wenfei , Kong Zhidong , Gao Wei , Miao Yi 
Published: March 2022

Abstract
Background: Clear cell renal cell carcinoma (ccRCC) is a common malignancy of the urinary system. With the increasing use of immune checkpoint inhibitors, the key roles of the tumour microenvironment (TME) in tumogenesis, tumour development and treatment resistance are gradually recognised. However, the understanding of ccRCC-TME and progression remains unclear.Methods: Transcriptome RNA-seq data and their corresponding clinical data were obtained from The Cancer Genome Atlas. Differentially expressed genes (DEGs) were identified by estimating stromal and immune scores. Common DEGs were identified by protein–protein interaction (PPI) network and univariate Cox regression analyses. Furthermore, Western blot was used to verify the expression level of candidate biomarkers in ccRCC cells. In addition, gene set enrichment analysis and immune infiltration analysis were performed to explore the mechanism of ccRCC and related immune cells. Finally, the correlation between the core genes and clinicopathological characteristics was assessed for potential clinical application.Results: IGLL5 was the only core gene found through PPI network intersection analysis and Cox regression analysis. IGLL5 was negatively correlated with the prognosis of patients with RCC. The IGLL5 protein expression was elevated in RCC. Clinically, 453 (86.45%) and 71 (13.55%) patients with RCC were stratified into the IGLL5≤16.33 and IGLL5>16.33 groups, respectively.Compared with IGLL5≤16.33, the IGLL5>16.33 group conferred a higher risk of developing high stage (odds ratio [OR], 2.759; 95% confidence interval [CI], 1.651–4.609) and high T stage (OR, 2.686; 95% CI, 1.615–4.467; all P<0.001).Conclusion: IGLL5 gene is a potential predictor of the high malignancy of RCC. It may serve as a potential immunotherapeutic target for RCC.
Clinicopathological features and incidence rate of oxyntic gland neoplasm: A single center retrospective study
Authors: Takao Toshitatsu , Asahara Hikari , Asahara Yumiko , Asahara Masakyo , Sakaguchi Hiroya , Yoshizaki Tetsuya , Ikezawa Nobuaki , Takao Madoka , Morita Yoshinori , Toyonaga Takashi , Kushima Ryoji , Kodama Yuzo 
Published: March 2022

Abstract
In this retrospective study, we aimed to examine oxyntic gland neoplasms (OGNs) identified in patients visiting an internal medicine clinic over a 40-month follow-up period. Additionally, we wished to clarify the clinicopathological characteristics and frequency of occurrence of these lesions. From December 1, 2017 to March 31, 2021, we performed 13,240 upper gastrointestinal endoscopies on 7488 patients. Of these, we identified 27 patients with 30 histopathologically confirmed OGNs, yielding a disease frequency of 0.36% (27/7488). Further, in three of 27 patients (11%), multiple simultaneous lesions had occurred. One of the 30 lesions (3.3%) was present in the antrum. Nine of the 9 patients (33%) had no history of Helicobacter pylori infection, and remaining 18 (67%) were either currently or previously infected. However, 27/30 lesions (90%) occurred in non-atrophied regions. After endoscopic treatment, submucosal infiltration was observed in eight (47%) of the 17 lesions that underwent histopathological evaluations, which showed no submucosal desmoplasia in all eight cases. Additional resection was not performed while patient progress has been monitored. No cases of recurrence have been reported. In conclusion, we found that the frequency of oxyntic gland neoplasms was much higher than previously reported.
Genetic characterization of a novel HIV-1 CRF01_AE/ CRF07_BC recombinant form among men who have sex with men in Baoding City, Hebei Province, China
Authors: Yang Xuegang , Zhu Huiying , An Weina , Zhao Jing , Lu Xinli , Sun Wenlong , Li Yongqin 
Published: March 2022

Abstract
Nowadays, a large number of unique recombinant forms have been found among sexual transmission populations in China. This study reported a novel HIV-1 second-generation unique recombinant form (URF), which was isolated from an HIV-positive man named BD201AJ who was infected through men who have sex with men(MSM) in Baoding City, Hebei province, China. This is different from a CRF01_AE/CRF07_BC recombinant strain from Hebei province, in China. The analysis result of the near full length genomic characterization showed that the genome comprises at least twelve interlaced segments, including six CRF07_BC and six CRF01_AE segments, with CRF07_BC as the main framework. No similar breakpoints between our strain and the other strains in the Los Alamos HIV database were observed. This highlights the importance of monitoring HIV-1 molecular epidemiological characteristics and the ongoing generation of recombinant viruses may provide an important insight for future analysis the recombination mechanism of HIV-1.
An In Vivo Definition of Brain Histamine Dynamics Reveals Critical Neuromodulatory Roles for This Elusive Messenger
Authors: Berger Shane , Baumberger Beatrice , Samaranayake S , Hersey M , Mena Sergio , Bain Ian , Duncan W , Reed M C , Nijhout F , Best J , Hashemi Parastoo 
Published: March 2022

Abstract
Histamine is well known for mediating peripheral inflammation, but histamine is also found in high concentrations in the brain where amongst other roles, this amine is thought to be neuromodulator. Neuromodulation is critical for brain function, yet histamine dynamics are very difficult to measure and thus several fundamental aspects of the mechanisms that control the extracellular and modulatory behavior of this messenger remain undefined. In this work we undertake the first in-depth characterization of in vivo histamine dynamics in real time using fast-scan cyclic voltammetry at carbon fiber microelectrodes. We measure electrically evoked histamine in the mouse hypothalamus and find that histamine release is sensitive to pharmacological manipulation at the level of synthesis, packaging, autoreceptor control of release, and metabolism. We find two breakthrough aspects of histamine modulation. First, there are differences in H3 receptor regulation of histamine between sexes showing that histamine release in female mice is more much tightly regulated than in male mice under H3 or inflammatory drug challenge. We hypothesize that this finding may contribute to hormone-mediated neuroprotection mechanisms in female mice. Second, we find that a high dose of a commonly available antihistamine, the H1 receptor inverse agonist diphenhydramine, rapidly decreases serotonin levels. This high dose is considered overdose; however, this finding highlights the sheer significance of better consideration of the modulatory nuances of histamine on serotonin.We, thus, present the first in depth in vivo characterization of fast histamine dynamics and highlight two breakthrough regulatory aspects of this elusive modulator. The implications of our study are new avenues to better understand and treat histamine related disorders of the brain (such as neuroinflammation), emphasizing that sex and modulation (of serotonin) are critical factors to consider when studying/designing new histamine targeting therapeutics.
An unusual location of an epidermoid cyst, the middle phalanx of the middle finger: A case report
Authors: (MS MRCS) *Dr. Dheeraj Makkar , (MS) Prof. Ravi Sahuta 
Published: March 2022

Abstract
Introduction:Epidermoid cysts are cutaneous cysts frequently observed among farmers, factory workers, carpenters, and tailors due to their occupations' predilection for repetitive minor hand trauma.Case Presentation:A 66-year-old male laborer presented to our hospital with a swelling on the palmar aspect of his right middle finger. The swelling was accompanied by pain and an inability to work over the previous four months. A 2.5 cm × 2.5 cm smooth, tender, immobile, non-compressible hard swelling was detected just above the middle phalanx during the clinical examination on the volar aspect of the right middle finger. The diagnosis was confirmed by histopathology after excision. The patient's range of motion improved significantly following surgery, and his pain subsided.Conclusion:We present a novel location for an epidermal inclusion cyst and the importance of histopathologic evaluation in this case. Additionally, we emphasize the significance of extensively checking the cyst's epithelium to guarantee complete capsule removal and rule out any additional pathology.
A Novel Model of Inducing Apoptosis of Mouse Cardiac Myocytes by Thermal Stimulation
Authors: Yang Jia , Sun Qihui , Ma Qingyun , Yu Qinhui , Liu Yanliang , Han Yuxiu , Xing Shuyan , Rong Rong , Yang Yong 
Published: March 2022

Abstract
Background: Thermal stimulation is a potent inducer of apoptosis in many cell lines. At present, water bath heating is often used to thermal stimulation-induced apoptosis, but this method has poor reproducibility and low efficiency. The aim of this study was to build a simple and reproducible apoptosis model caused by thermal stimulation. Methods and Results: The apoptosis and necrosis rate were detected under different thermal stimulation conditions by flow cytometer. We also analyzed the difference of growth curves between the thermal stimulation model group and the normal group. According to the cell growth curve, the cell re-culture time after thermal stimulation was preliminarily detected, and the reasonability of the time was verified by flow cytometer. The conditions for establishing the model are: PCR calculated temperature control system with a gradual heating pattern, 37°C, 2 min; 38°C, 2 min; 39°C, 2 min; 40°C, 2 min; 41°C, 2 min; 42°C, 2 min; 43°C, 60 min; 42°C, 2 min; 41°C, 2 min; 40°C, 2 min; 39°C, 2 min; 38°C, 2 min; 37°C, 2 min. In this way, the proportion of cell apoptosis is higher than that of cell necrosis with satisfied reproducibility. Conclusions: The present work provide a new method to induce cell apoptosis by thermal stimulation.
Molecular Viewpoint for the Stability Mechanism of SiO2/SDS Dispersion
Authors: Liu Nannan , Chen Yan , Chen Xinglong , Du Hui , Xu Hui , Zhang Yingnan , Shao Minglu , Zhao Huijun , He Yanfeng , Ju Binshan , Sun Zheng 
Published: March 2022

Abstract
The dispersion of silica dioxide (SiO2)/sodium lauryl sulfate (SDS) have been widely used in the oilfield development and the remaining oil displacement, but its instability is still a problem in the practical engineering application. In this paper, the mechanism of foam stability was investigated by combining the measurement of interfacial energy of nanoparticles at the gas-liquid interface with the dynamics simulation of molecular diffusion. The results showed that the addition of SiO2 nanoparticles improved the interfacial energy and interfacial activity at the gas-liquid interface, meanwhile limited the movement of SDS molecules and water molecules, which was beneficial for the foam stability. Notably, the addition of modified SiO2 nanoparticles further enhanced the interfacial energy at the gas-liquid interface and strengthened the restriction of water/SDS molecular movement, thereby slowing down the drainage and decay of the foam dispersions. The mechanism investigation was of benefit for foam flooding engineering application.
Diabetes exacerbated sepsis-induced intestinal injury by promoting M1 Macrophage Polarization via miR-3061/Snail1 signaling
Authors: Tan Fang , Cao Yuling , Zheng Lei , Wang Tao , Zhao Shuhua , Chen Jiong , Pang Changji , Xia Zhengyuan , Li Ningning , Chi Xinjin 
Published: March 2022

Abstract
Background
Macrophages play the important roles in the diabetes and sepsis-related intestinal injury. Accumulating evidence suggests that transcription factors act as the fundamental link between macrophage polarization and tissue injury. However, the underlying mechanisms of transcription factors regulating macrophage polarization-related intestinal injury remain unclear under diabetes and sepsis conditions.
Methods
The cecal ligation and puncture (CLP)-induced sepsis models were established in both wild type (WT) and diabetic male mice. Clodronate liposome (Cls) was used to deplete macrophage. HE staining, inflammatory cytokines (TNF-α, IL-1β and IL-6) and intestinal mucosal barrier function markers (occludin, ZO-1, LPS and iFABP) were used to elevated intestinal damage. MicroRNA-array, RNA-seq and bioinformatic analysis were performed to detect the microRNA and mRNA expression and the potential regulation mechanism. In vitro, high glucose and LPS, miR-3061 mimics and Snail siRNA stimulation of RAW264.7 cells were cultured for further mechanism studies. Luciferase reporter assay was used to confirm the interplay between microRNA and its target genes.
Results
Compared to WT CLP mice, the diabetic CLP mice showed severe intestinal damage characterized by significantly increased Chui’s scores, expression of inflammatory cytokines (TNF-α, IL-1β and IL-6), serum LPS and iFABP concentration and reduced tight junction protein occluding and ZO-1 levels. Macrophage deplettion exhibited reverse the intestinal damage caused by CLP. The bioinformatic analysis identified that miR-3061/Snail1 might be a potential regulation axis of macrophage polarization. Furthermore, high glucose and LPS stimulation increased M1 macrophage and reduced levels of miR-3061, which was negatively associated with Snail1 in RAW264.7 cells. Mechanistic researches demonstrated that miR-3061 regulated macrophage polarization by targeting Snail1 mRNA 3′-untranslated region (3’‐UTR). Moreover, miR-3061 overexpression suppressed Snail1 expression, inhibited M1 macrophage and inflammatory cytokines.
Conclusion
This study elucidated that diabetes exacerbated sepsis-induced intestinal injury by promoting M1 macrophage polarization, and further demonstrated that the miR-3061/Sani1 axis may be the potential target of macrophage polarization.
The Effect of Variation in Dietary Cation-Anion Difference During the Transition Period of Brown Swiss Dairy Cows on Calcium Status, Blood Metabolites and Rumen Activity
Authors: Hassanien H. E. M. , Abdel-Raouf E. M. , Mahmoud A. M. M. 
Published: March 2022

Abstract
The objective of this study was to determine the effects of varying pre- and postpartum DCAD diets on serum total calcium, ionized calcium, blood and ruminal fluid metabolites, and milk production in prepartum and postpartum Holstein cows. Fifty-four multiparous dry Brown Swiss cows n = 54, were enrolled in a completely randomized block experimental design at 29 days prior to expected parturition through 86 days in milk. A 3 x 2 factorial arrangement of treatments was utilized. Three DCAD levels were fed precalving (0, -120 and − 200 mEq/kg DM, n = 18 cows per treatment) and two DCAD levels were fed post calving (+ 200 and + 400 mEq/kg DM, n = 27 cows per treatment). Prepartum urine pH was lower for cows fed − 200 DCAD compared with those fed − 120 or 0 DCAD. Postpartum urine pH was higher for cows fed + 400 mEq/kg compared to cows fed + 200 mEq/kg DCAD. Prepartum serum total calcium, ionized calcium, and hydroxyproline was highest for cows fed − 200 DCAD compared to those fed − 120 and 0 DCAD. Parathyroid hormone was highest for cows fed 0 DCAD compared to those fed − 120 and − 200 DCAD. Prepartum dry matter intake (DMI) was lower for − 200 and − 120 DCAD compared with 0 DCAD. Postpartum DMI was not different among treatments. Pre- and postpartum DCAD treatments did not affect total milk yield or milk fat, but percentage of milk protein increased in cows fed the negative prepartum DCAD treatments.
Effects of trade openness on environmental quality: Evidence from developing countries
Authors: Thuy Diem Pham Thi , Nguyen Hoai Trong 
Published: March 2022

Abstract
This paper uses the Bayesian model averaging approach to investigate the effects of trade openness on environmental quality for a sample of 64 developing countries during the period 2003–2017. Differing from Antweiler et al. (2001), Cole and Elliott (2003), and Managi et al. (2009), we bridge the gap by the inclusion of financial openness and renewable energy consumption as the conditioning factors that might be crucial for environmental quality. The empirical results reveal that trade openness in developing countries does not cause environmental degradation. Besides trade openness, our findings provide strong evidence in favor of financial openness and renewable energy consumption as two of the most important determinants of environmental quality by reducing carbon dioxide emissions. Meanwhile, inward FDI stock to domestic capital stock and income have harmful effects on environmental quality in the context of developing countries.
Functional Analysis of BrUFO in Chinese Cabbage in Response to Plasmodiophora Brassicae
Authors: Zhang Bo , Feng Hui , Ge Wenjie , Wang Xinlei , Zhang Jing , Ji Ruiqin 
Published: March 2022

Abstract
Chinese cabbage is one of the most important vegetable crops in China. However, the yield is seriously affected by clubroot disease, caused by infection with Plasmodiophora brassicae . In our previous study, the Bra039878 protein was found to be significantly upregulated in diseased roots of Chinese cabbage after inoculation with P . brassicae . Herein, to explore its function, a full-length cDNA of BrUFO was cloned and analysed. The results revealed high homology with Arabidopsis UNUSUAL FLORAL ORGANS ( UFO ), hence we named it BrUFO . Quantitative real-time polymerase chain reactions (qRT-PCR) and in situ hybridisation analysis showed that expression of BrUFO was lower in the resistant plants than in susceptible plants, suggesting that BrUFO is related to resistance to clubroot disease. Subcellular localisation analysis showed that BrUFO was expressed in the nucleus. Virus-induced gene silencing (VIGS) analysis showed that silencing of BrUFO reduced the incidence of clubroot disease. Six proteins interacting with BrUFO were screened by Y2H assay, and two ( Bra038955 and Bra021273 ) were confirmed to strongly interact with BrUFO by Y2H point-to-point verification. Bra038955 is a GDSL-motif esterase/acyltransferase/lipase, but the function of Bra021273 is unknown. Thus, BrUFO may play a key role in regulating disease resistance to clubroot by interacting with GDSL .
Chronic Pre-Treatment of Kombucha Tea Protects Brain Injury induced by Ischemia/Reperfusion in Global Brain Ischemia
Authors: Mesgari-Abbasi Mehran , Vaezi Fatemeh Eskandari , Hossienzadeh Fezzeh 
Published: March 2022

Abstract
Cerebral ischemia is an important factor in developing neurological damage without appropriate medical treatment. Kombucha tea(KT) is a fermented beverage that produces many compounds with antioxidant power with potential health effects. This study was performed to evaluate the effect of pre-treatment of kombucha tea on brain edema, neurobehavioral, and oxidative stress in the global brain ischemia model. Adult male Wistar rats were divided into four groups include; the sham, the ischemic, and the KT-treated-ischemic groups. KT was administrated two weeks before inducing transient global cerebral ischemia at two different dosages (one and two ml/kg/day, gavage). The global brain ischemia was induced by blocking the common carotid arteries for one hour, followed by 24 hours of reperfusion. At the end of reperfusion, neurobehavioral studies were done. Brain edema was determined by dry-wet methods. Malondialdehyde (MDA), total antioxidant capacity (TAC), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) levels were measured in the serum and the brain tissue. Our results showed that pre-treatment of KT significantly reduced brain edema and MDA levels in the blood and the brain. It significantly increased the total TAC and GPx levels in the blood and the brain in ischemic rats treated with KT. The CAT activity significantly increased in the blood, but it didn’t significantly change the brain. KT of pre-treatment significantly ameliorated neurobehavioral defects in the ischemic rats. Our data demonstrated that pre-treatment of KT maybe protect the brain against ischemic-reperfusion injury through a decrease in the production of lipid peroxidation and an increase in the capacity of antioxidants defense.