bluuebunny/crossref_metadata_2025_split huggingface dataset:
AlG3 Induces AURKA to Promote Laryngeal Squamous Cell Carcinoma Metastasis
Authors: YANG Li-Yun , Qiao Peipei , Hu An , huang shuixian 
Published: January 2022

Abstract
Objectives To investigate the relationship between ALG3 and AURKA, the expression and potential prognostic value of ALG3 in LSCC, and tothenexplorethe impact of ALG3 in tumorigenic effects.Methods Co-immunoprecipitation assay was detected the relationship between ALG3 and AURKA, Rt-PCR and Western blot was detected the expression of related mRNA and proteins.CCK8 assay, plate colony formation assay Cells, wound healing, migration and invasion assays were used to examine the abilityof proliferation, movement, migration and invasion of LSCC cells.Results ALG3 immediately induced AURKA to promote LSCC metastasis. Moreover, ALG3 highly expressed in LSCC tissues and cells and the expression of ALG3 was positively relatedto tumor size,lymphatic metastasis and poor clinical prognosis. Furthermore, knockdown ALG3 in LSCC cells remarkablyrestraincellular proliferation, migration and invasioninvitroand vivo.Conclusion AlG3 induced AURKA to promote LSCC metastasis and ALG3 maybe potential prognostic value for LSCC.Brief AbstractAlG3 induces AURKA to promote laryngeal squamous cell carcinoma metastasis
Machine Learning-Based Optimization of Pre-Symptomatic COVID-19 Detection Through Smartwatch
Authors: Cho Hyeong Rae , Kim Jin Hyun , Yoon Hye Rin , Han Yong Seop , Kang Tae Seen , Choi Hyunju , Lee Seunghwan 
Published: January 2022

Abstract
Patients with weak or no symptoms accelerate the spread of COVID-19 through various mutations and require more aggressive and active means of validating the COVID-19 infection. More than 30% of patients are reported as asymptomatic infection after the delta mutation spread in Korea. It means that there is a need for a means to more actively and accurately validate the infection of the epidemic via pre-symptomatic detection, besides confirming the infection via the symptoms. Mishara et al.[1] reported that physiological data collected from smartwatches could be an indicator to suspect COVID-19 infection. It shows that it is possible to identify an abnormal state suspected of COVID-19 by applying an anomaly detection method for the smartwatch’s physiological data and identifying the subject’s abnormal state to be observed. This paper proposes to apply the One Class-Support Vector Machine (OC-SVM) for pre-symptomatic COVID-19 detection. We show that OC-SVM can provide better performance than the Mahalanobis distance-based method used by Mishara et al.[1] in three aspects: earlier (23.5%-40% earlier) and more detection (13.2%-19.1% relative better) and fewer false positives. As a result, we could conclude that OC-SVM using RHR (Resting Heart Rate) with 350 and 300 moving average size is the most recommended technique for COVID-19 pre-symptomatic detection based on physiological data from the smartwatch.
Breastfeeding and Overweight/Obesity Among Children and Adolescents: A Cross-Sectional Study
Authors: Liu Fange , Lv Di , Han Wenchao , Wang Lumin , Feng Xiaoyu , Zhang Rongjun , Liu Wendong 
Published: January 2022

Abstract
Background Overweight and Obesity are a major public health crisis among children and adolescent and contributes to significant economic burden. We aim to aims to investigate the relationship between breastfeeding duration and overweight and obesity in children and adolescents aged 6 to 16 from Shibei District of Qingdao, China in 2017. Methods Data on breastfeeding and children and adolescent anthropometric measurements were collected in a cross-sectional study in Qingdao, China (n=11732). Differences between groups were compared using t-test and chi-square test. Multiple linear regression model and generalized linear model were used to analyze the relationship between duration of breastfeeding and BMI. Logistic regression analysis model was used to analyze the relationship between breastfeeding duration and the prevalence of overweight and obesity. Other factors potentially related to children and adolescents’ overweight and obesity were also considered. Results The prevalence of overweight and obesity was 35.21%, overweight and obesity being 15.45% and 19.76%, respectively. After adjusting covariates, there was a significant negative correlation between breastfeeding duration and BMI in children and adolescents (β=-0.025, 95%CI: -0.033, -0.005, P<0.01). Among boys, the BMI in childhood and in adolescence of those who have been breastfed for more than 12 months is significantly lower than others whose breastfeeding duration was less than 12 months (β=-0.440, 95%CI -0.655, -0.224, P<0.01). After classifying subjects into age groups, among boys aged 9 to 11, breastfeeding has a particularly positive effect on the prevalence of obesity (OR=0.978, 95%CI: 0.958,0.999, P<0.05), and the prevalence of obesity was lower among those whose breastfeeding duration was more than 12 months (OR=0.787, 95%CI: 0.653,0.975, P<0.05). Conclusion Breastfeeding can significantly reduce the prevalence of overweight and obesity among children and adolescents aged 6 to 16. And those who were breastfed for more than 12 months had a lower risk of developing overweight and obesity, especially in boys between the age of 9 to 11.
Integrated Scheduling Algorithm For Complex Products Based On The Dynamic Subtree Operation Set Inverse Coding
Authors: Wang Qian , Xie Zhiqiang , Gao Yilong , Yu Xu 
Published: January 2022

Abstract
For the previous integrated scheduling algorithms for complex products, the migration time of operations between machines is ignored or just included in the processing time of its adjacent operations, which leads to inaccurate scheduling results and is difficult to meet the needs of the actual production scheduling environment. In this paper, based on the framework of a genetic algorithm, an integrated scheduling algorithm for complex products based on the dynamic subtree operation set inverse coding. Firstly, an inverse coding method based on the dynamic subtree operation set is proposed, which can ensure the legitimacy of the initial individuals and enhance the quality of the initial population. Secondly, based on the crossover vector, a single-point crossover method and a multi-point crossover method are proposed, both of which can ensure that the priority constraints among the same machine operation in the generated individuals will not be destroyed. Then, a mutation method based on the mutant row vector and mutant column vector is proposed to ensure the feasibility and diversity of the offspring individuals. Finally, a pre-decoding method based on device idle events driven and a conversion strategy of positive sequence schemes based on the completion time flipping are shown. The performance of the proposed algorithm is verified by several groups of comparative experiments.
PM2.5 Exposure and Cervical Cancer Survival in Liaoning Province, Northeastern China
Authors: Liu Guangcong , Yang Zhuo , Wang Chenyu , Wang Danbo 
Published: January 2022

Abstract
Particulate matters with a diameter of 2.5 µm or less (PM2.5) were frequently reported to be associated with increased cancer incidences, but few studies have explored the associations between PM2.5 exposure and cancer survival. We retrospectively analyzed the association between PM2.5 exposure and the overall survival (OS) of cervical cancer patients who resided in 14 urban areas of the Liaoning Province, Northeastern China during January 2014-October 2021. Patients from urban areas who finished the recommended treatments with complete follow-up information were included. The PM2.5 monitoring data of each urban area of Liaoning Province was retrieved, and individual exposure to PM2.5 after diagnosis was calculated as the average daily concentration in the residence from the discharged day to death day or the last follow-up day. Log-rank tests and cox-regression were performed to examine the relationships between PM2.5 exposure and cervical cancer survival. A total of 1753 cervical cancer patients were finally included, among whom 804 (45.9%) were from Shenyang City, the capital of Liaoning Province. The median average daily concentration of PM2.5 that the patients were exposed to was 45.0 (interquartile range 38.2-50.0) µg/m3. Both log-rank tests (grouped by quartiles, p<0.001) and cox-regression (continuous, HR=1.06, 95%CI 1.04-1.08) indicated that PM2.5 was significantly associated with shorter OS. Sensitivity analysis also confirmed the robustness of our findings. For the subgroup analysis, only the OS of stage II and stage III patients were associated with PM exposure. Our findings gave an insight that PM2.5 exposure was associated with shorter OS of cervical cancer patients.
Ultra-Broadband Dispersion-Manipulated Dielectric Metalenses by Nonlinear Dispersive Phase Compensation
Authors: Hu Yueqiang , Jinag Yuting , Zhang Yi , Lai Jiajie , He Peng , Ou Xiangnian , Li Ling , Duan Huigao 
Published: January 2022

Abstract
Dispersion decomposes compound light into monochromatic components at different spatial locations, which needs to be eliminated in imaging but utilized in spectral detection. Metasurfaces provide a unique path to modulate the dispersion by adjusting the structural parameters without changing the material as required for refractive elements. However, the common linear phase compensation does not conform to the dispersion characteristics of the meta-unit, which limits the dispersion modulation in broader wavelength bands. Here, we propose a nonlinear dispersive phase compensation approach to design polarization-insensitive achromatic metalenses from 400 nm to 1000 nm constructed with single-layer high aspect ratio nanostructures. This band matches the response spectrum of a typical CMOS sensor for both visible and near-infrared imaging applications without additional lens replacement. Moreover, the capability of the approach in achieving arbitrary dispersion modulation is demonstrated for potential applications such as chromatography imaging and spectral detection.
Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat
Authors: Patel Sveta , Purohit Nikisha , Sapra Palak , Solanki Hitesh , Bishoyi Ashok Kumar 
Published: January 2022

Abstract
Genetic diversity analysis contributes to the conservation, protection and utilization of genetic resources toward efficient management of germplasm. In this investigation, Inter Simple Sequence Repeats (ISSR) markers were used to analyze the genetic diversity of 32 genotypes of Acmella paniculata collected from 12 different states of Gujarat, India. There were 113 loci amplified by 15 different ISSR markers with an average of 7.53 loci per primer. From the total 113 amplicons; 81 were polymorphic, 43 were monomorphic and 9 were found to be unique showing an average of 60.18% polymorphism. The primer (CT)8T produced a high value (0.65) of polymorphic information content (PIC); whereas, primer (TC)8RA produced a low value (0.21). The resolving power (RP) value of the primers ranged from 5.25 in primer (TC)8RA to 23.19 in primer (AC)8YT with an average of 12.17. The Jaccard’s similarity coefficients value ranged from 0.48 to 0.94. The highest percentage of similarity (94%) was found between accessions AP6 and AP16 which were collected from Panchmahal and Narmada respectively, as they are geographically closely located. Whereas, accessions AP2 from Banaskantha and AP7 from Panchmahal were most distantly related with the similarity value of 48%, as expected to their collection locations. The study revealed that there is no correlation between geographical distance and genetic diversity among the populations studied. The resultant data generated from ISSR molecular marker-based genetic diversity analysis of A. paniculata in Gujarat can provide a reference for the conservation and efficient management of the important Indian genetic resources.
Assessment of Dose Perturbations for Metal Stent in Photon and Proton Radiotherapy Plans for Hepatocellular Carcinoma
Authors: Lee Boram , Cho Sungkoo , Park Hee Chul , Kang Sang-Won , Kim Jae-Sung , Chung Jin-Beom 
Published: January 2022

Abstract
Background: The present study aimed to investigate the dosimetric impact of metal stent for photon and proton treatment plans in hepatocellular carcinoma.Methods: Dose perturbation caused by a metal stent included in the photon and proton treatment of hepatocellular carcinoma was evaluated with comparing Eclipse and RayStation treatment planning system (TPS) to Monte Carlo (MC). Photon and proton plans were created using anterior-posterior/posterior-anterior (AP/PA) fields using a 6 MV beam and AP/PA fields of a wobbling beam using 150 MeV and a 10 cm ridge filter. The difference in dose distributions and dosimetric parameters were compared depending on the stent's positions (the bile duct (GB) and the intestinal tract (GI)) and angles (0°, 45°, and 90°). In addition, the dose variation in the target volume including the stent was comparatively evaluated through dose volume histogram (DVH) analysis.Results: The percentage differences in the dosimetric parameters calculated by MC ranged from -7.0 to 3.9% for the photon beam and -33.7% to 4.3% for the proton beam, depending on the angle at which the GB and GI stents were placed. A maximum difference was observed at the minimum dose (Dmin), which was shown in both photon and proton plans in the GB and GI stents deployed at an incidence angle of 90°. The differences in these parameters were greater in the proton plan than in photon plan. The target volume showed various dose variations depending on positions and angles of stent for both beams. Compared with the no-stent, the doses within the target volume containing the GI and GB stents for the photon beam were overestimated in the high-dose area at 0°, nearly equal within 1% at 45°, and underestimated at 90°. These doses to the proton beam were underestimated at all angles, and the amount of underdose to the target volume increased with the increase of the stent’s angle. However, the difference was much greater with the proton plan than with the photon plan.Conclusions: Dose perturbations within the target volume due to the presence of the metal stent were not observed in the TPS calculations for photon and proton beams, but MC were used to confirm that there are the dose variation within the target volume. The MC results found that delivery of the treatment beam avoiding the stent was the best method to prevent underdose of the target volume.
Anti-VEGF Maintains The Function of Retinal Pigment Epithelium In Patients With Retinal Vein Occlusion
Authors: Yang Qian , Luo Dawei , Qiu Qinghua , Xu Guo-Tong , Zhang Jingfa 
Published: January 2022

Abstract
Background: To evaluate the drainage function of retinal pigment epithelium (RPE) in patients with macular edema secondary to retinal vein occlusion (RVO-ME) after anti-vascular endothelial growth factor (anti-VEGF) treatment by optical coherence tomography angiography (OCTA).Patients and Methods: Twenty-eight eyes with RVO-ME were imaged. Best-corrected visual acuity (BCVA), the area of the foveal avascular zone (FAZ), the nonperfusion areas (NPAs), the central macular thickness (CMT), the vascular density of the superficial/deep capillary plexus (SCP/DCP), the height of subretinal fluid (SRF), and the number of hyperreflective foci (HRF) were compared before and after treatments.Results: Compared with pre-treatment, the CMT, NPAs, number of HRF and the height of SRF were decreased significantly after anti-VEGF treatment. There was no difference for BCVA, vascular density and FAZ area.Conclusion: The absorption of SRF and the decrease of HRF indicated the restoration of RPE drainage function and anti-inflammation by anti-VEGF treatment.
From head to rootlet: comparative transcriptomic analysis of a rhizocephalan barnacle Peltogaster reticulata (Crustacea: Rhizocephala)
Authors: Nesterenko Maksim A. , Miroliubov Aleksei A. 
Published: January 2022

Abstract
Rhizocephalan barnacles stand out in the diverse world of metazoan parasites. The body of a rhizocephalan female is modified beyond revealing any recognizable morphological features, consisting of the interna, the system of rootlets, and the externa, a sac-like reproductive body. Moreover, rhizocephalans have an outstanding ability to control their hosts, literally turning them into “zombies”. Here we performed the first comparative transcriptomic analysis of rhizocephalan (Peltogaster reticulata) adult female body parts and established the spatial heterogeneity of gene expression in different regions. This resulted in an unexpected discovery of germ cells in the lumen of interna, which was additionally validated by histological methods. Our finding of germ cells in interna deprives externa from the exclusive role in propagation and renders it a simple brooding chamber. Furthermore, we confirmed that the neurons of the host are attracted to the rootlets of the interna, indicating a great intimacy of host-parasite relationship. Finally, based on the continual expression of development-associated genes, we suggest that rhizocephalans “got stuck in the metamorphosis”, even in their reproductive stage.
Microplastics in Water, Sediments and Macroinvertebrates in a Small River of NW Spain
Authors: Álvarez-Troncoso Romina , Gutiérrez David , Villar Iria , Ehlers Sonja , Soto Benedicto , Mato Salustiano , Garrido Josefina 
Published: January 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
The GAs Extraction and Analyses system (GAEA) for immediate extraction and measurements of volatiles in the Hayabusa2 sample container
Authors: Miura Yayoi N. , Okazaki Ryuji , Takano Yoshinori , Sakamoto Kanako , Tachibana Shogo , Yamada Keita , Sakai Saburo , Sawada Hirotaka 
Published: January 2022

Abstract
Hayabusa2 returned surface samples from the C-type near-Earth asteroid (162173) Ryugu to Woomera, South Australia, in December 2020. The samples returned from Ryugu are expected to contain volatile components reflecting its primitive nature and solar wind components due to exposure to space. Such volatiles may partly be released inside the sealed sample container enclosing Ryugu samples due to particle destruction or container heating in a contingency case. In order to collect and analyze volatiles released in the container prior to the container opening, we set up a gas extraction and analyses system (GAEA: GAs Extraction and Analyses system). The system requires ultra-high vacuum condition, small vacuum line volume to minimize dead volume and simple configuration as well as having an interface to connect the container. The system includes gas bottles for passive collection of volatiles at room temperature and bottles for active collection at liquid nitrogen temperature. A quadrupole mass spectrometer is installed to analyze gases in the vacuum line, and a non-evaporative getter pump is also used when noble gases are analyzed. The rehearsal operation of GAEA was made at ISAS/JAXA as well as transportation tests in Japan. In November 2020, it was transported safely to the Quick Look Facility (QLF) in Woomera. It was set up at the QLF and worked well as planned for collection and analysis of gas components from the returned sample container. Here we report the concept, design and calibration results for the GAEA and an outline of analytical protocols applied in Woomera.
Extracellular ATP Binding to P2Y1 Receptors Prevents Glutamate-Induced Excitotoxicity: Involvement of Erk1/2 Signaling Pathway to Suppress Autophagy
Authors: Xiong Yiping , Zhou Duanyang , Zheng Kai , Bi Wenchuan , Dong Yun 
Published: January 2022

Abstract
Glutamate-induced neuroexcitotoxicity causes neuronal death in neurodegenerative diseases including Alzheimer’s disease and Parkinson’s disease. Extracellular ATP exerts a wide variety of functions, such as attenuating Aβ-mediated toxicity, inhibiting NMDA receptor subunit combinations, and aggravating ischemic brain injury. However, the effect of extracellular ATP on glutamate-induced neuroexcitotoxicity remains largely unknown. Herein, we showed that extracellular ATP prevented the glutamate-induced excitotoxicity via binding to its P2Y1 receptors. We found that excessive glutamate triggered cellular ROS overproduction and mitochondrial membrane potential damage, which were significantly attenuated by extracellular ATP. Besides, glutamate activated autophagy, as illustrated by the increased protein level of autophagic marker LC3II and decreased level of p62, and glutamate-induced neuroexcitotoxicity could be completely abolished by autophagy inhibitor chloroquine. In addition, we revealed that extracellular ATP activated Erk1/2 signaling to suppress autophagy and to exert its neuroprotective effects, which was further reduced by autophagy agonist rapamycin and the selective Erk1/2 inhibitor PD0325901. Taken together, our findings suggest that extracellular ATP binding to P2Y1 receptors protected against glutamate-induced excitotoxicity via Erk1/2-mediated autophagy inhibition, implying the promising potential of ATP for the treatment of neurodegenerative disorders.
Towns and Trails Drive Carnivore Movement Behaviour, Resource Selection, and Connectivity
Authors: Whittington Jesse , Hebblewhite Mark , Baron Robin W. , Ford Adam T. , Paczkowski John 
Published: January 2022

Abstract
Background. Global increases in human activity threaten connectivity of animal habitat and populations. Protection and restoration of wildlife habitat and movement corridors require robust models to forecast the effects of human activity on movement behaviour, resource selection, and connectivity. Recent research suggests that animal resource selection and responses to human activity depend on their behavioural movement state, with increased tolerance for human activity in fast states of movement. Yet, few studies have incorporated state-dependent movement behaviour into analyses of Merriam connectivity, that is individual-based metrics of connectivity that incorporate landscape structure and movement behaviour. Methods. We assessed the cumulative effects of anthropogenic development on multiple movement processes including movement behaviour, resource selection, and Merriam connectivity. We simulated movement paths using hidden Markov movement models and step selection functions to estimate habitat use and connectivity for three landscape scenarios: reference conditions with no anthropogenic development, current conditions, and future conditions with a simulated expansion of towns and recreational trails. Our analysis used 20 years of grizzly bear (Ursus arctos) and gray wolf (Canis lupus) movement data collected in and around Banff National Park, Canada. Results. Carnivores increased their speed of travel near towns and areas of high trail and road density, presumably to avoid encounters with people. They exhibited stronger avoidance of anthropogenic development when foraging and resting compared to travelling and during the day compared to night. Wolves exhibited stronger avoidance of anthropogenic development than grizzly bears. Current development reduced the amount of high-quality habitat between two mountain towns by more than 35%. Habitat degradation constrained movement routes around towns and was most pronounced for foraging and resting behaviour. Anthropogenic development reduced connectivity by more than 80%. Habitat quality and connectivity further declined under a future development scenario. Conclusions. Our results highlight the cumulative effects of anthropogenic development on carnivore movement behaviour, habitat use, and connectivity. Our strong behaviour-specific responses to human activity suggest that conservation initiatives should consider how proposed developments and restoration actions would affect where animals travel and how they use the landscape.