bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conformation and energy investigation of microtubule longitudinal dynamic instability induced by natural products
Authors: Chu Yanyan , Tian Zhenhua , Yang Mengke , Li Wenbao 
Published: December 2022

AbstractThe natural products plinabulin, docetaxel, and vinblastine are microtubule targeting agents (MTAs). They have been used alone or in combination in cancer treatment. However, the exact nature of their effects on microtubule (MT) polymerization dynamics is poorly understood. To elucidate the longitudinal conformational and energetic changes during MT dynamics, a total of 140 ns molecular dynamic simulations combined with binding free energy calculations were performed on seven tubulin models. The results indicated that the drugs disrupted MT polymerization by altering both MT conformation and binding free energy of the neighboring tubulin subunits. The combination of plinabulin and docetaxel destabilized MT polymerization due to bending MT and weakening the polarity of tubulin polymerization. The new combination of docetaxel and vinblastine synergistically enhanced MT depolymerization and bending, while plinabulin and vinblastine had no synergistic inhibitory effects. The results were verified by the tubulin assembly assay. Our study obtained a comprehensive understanding of the action mechanisms of three natural drugs and their combinations on MT dynamic, provided theoretical guidance for new MTA combinations, and would promote the optimal use of MTA and contribute to developing new MTAs as anticancer agents.
Subtypes of mathematical learning disability and their antecedents: A cognitive diagnostic approach
Authors: Ouyang Xiangzi , Zhang Xiao , Räsänen Pekka , Koponen Tuire , Lerkkanen Marja‐Kristiina 
Published: December 2022

AbstractUsing cognitive diagnostic modeling (CDM), this study identified subtypes of mathematics learning disability (MLD) based on children's numerical skills and examined the language and spatial precursors of these subtypes. Participants were 99 MLD children and 420 low achievers identified from 1839 Finnish children (966 boys) who were followed from preschool (age 6) to fourth grade (2007–2011). Five subtypes were identified: the arithmetic fluency deficit only subtype, the counting deficit subtype, the pervasive deficit subtype, the symbolic deficit subtype, and the counting and concept deficit subtype. Different subtypes depended on different constellations of language and spatial deficits. Findings highlight the effectiveness of CDM in identifying MLD subtypes and underscore the importance of understanding the specific deficits and antecedents of the subtypes.
Clinical, methodology, and patient/carer expert advice in pediatric drug development by conect4children
Authors: Cheng Katharine , Mahler Fenna , Lutsar Irja , Nafria Escalera Begonya , Breitenstein Stefanie , Vassal Gilles , Claverol Joana , Noel Palacio Nuria , Portman Ron , Pope Gavin , Bakker Martijn , van der Geest Tessa , Turner Mark A. , de Wildt Saskia N. ,   c4c Expert Group Leads
Published: December 2022

AbstractMany medicines are used “off‐label” in children outside the terms of the license. Feasible pediatric clinical trials are a challenge to design. Conect4children (c4c) is an Innovative Medicines Initiative project to set up a pan‐European pediatric clinical trial network aiming to facilitate the development of new medicines for children. To optimize pediatric trial development by promoting innovative trial design, c4c set up a European multidisciplinary advice service, including the voice of young patients and families, tailored to industry and academia. A network of experts was established to provide multidisciplinary advice to trial sponsors. Experts were selected to join clinical and innovative methodology expert groups. A patient and public involvement (PPI) database, to include the expert opinion of patients and parents/carers was formed. A stepwise process was developed: (1) sponsors contact c4c, (2) scoping interview takes place, (3) ad hoc advice group formed, (5) advice meeting held, and (6) advice report provided. Feedback on the process was collected. Twenty‐four clinical and innovative methodology expert groups (>400 experts) and a PPI database of 135 registrants were established. As of September 30, 2022, 36 advice requests were received, with 25 requests completed. Clinical and methodology experts and PPI representatives participated in several advice requests. Sponsors appreciated the advice quality and the multidisciplinary experts from different countries, including experts not known before. Experts and PPI participants were generally satisfied with the process. The c4c project has shown successful proof of concept for a service that presents a new framework to plan innovative and feasible pediatric trials.
The vicious circle of UHRF1 down‐regulation and KEAP1/NRF2/HO‐1 pathway impairment promotes oxidative stress‐induced endothelial cell apoptosis in diabetes
Authors: Guo Zi , Wan Xinxing , Luo Yufang , Liang Fang , Jiang Siwei , Yuan Xiuhong , Mo Zhaohui 
Published: December 2022

AbstractBackgroundOxidative stress is recognized as a key factor in the induction of endothelial dysfunction in diabetes. However, the specific mechanisms have not been fully elucidated. We herein hypothesized that ubiquitin‐like containing PHD and RING finger domains 1 (UHRF1) might have a role in oxidative stress‐induced endothelial cell (EC) apoptosis in diabetes.MethodsWestern blot, qPCR, wound healing assay, apoptosis assay, reactive oxygen species (ROS) detection, dual‐luciferase reporter assay, methylation‐specific PCR, bisulfite sequencing PCR and chromatin immunoprecipitation assay were performed.ResultsUHRF1 expression levels were significantly decreased in endothelial colony‐forming cells derived from peripheral blood of participants with type 2 diabetes compared with individuals without diabetes. ECs treated with high glucose, palmitate or hydrogen peroxide in vitro also exhibited decreased UHRF1 protein levels. Silencing of UHRF1 led to decreased migration ability and increased apoptosis and ROS production in ECs, which might be related to impaired Kelch‐like ECH‐associated protein 1 (KEAP1)/nuclear factor erythroid 2‐related factor 2 (NRF2)/haeme oxygenase‐1 pathway. Mechanistically, UHRF1 is closely implicated in epigenetic regulation of chromatin modification status at KEAP1 genomic locus via histone acetylation. NRF2 down‐regulation in turn inhibits UHRF1 protein level, which might be due to increased ROS generation.ConclusionDiabetes‐induced oxidative stress can mediate down‐regulation of UHRF1, which enhances ROS production by regulating KEAP1/p‐NRF2 pathway through histone acetylation and might also form a self‐perpetuating feedback loop with KEAP1/p‐NRF2 to further promote oxidative stress‐induced apoptosis of ECs in diabetes.
Global burden of gout in 1990–2019: A systematic analysis of the Global Burden of Disease study 2019
Authors: Jeong Yeon Jae , Park Seoyeon , Yon Dong Keon , Lee Seung Won , Tizaoui Kalthoum , Koyanagi Ai , Jacob Louis , Kostev Karel , Dragioti Elena , Radua Joaquim , Stickley Andrew , Oh Hans , Shin Jae Il , Smith Lee 
Published: December 2022

AbstractBackground and AimsAlthough gout is one of the most common rheumatic diseases, world data are lacking because most studies have focused on industrialized countries. Therefore, we aimed to investigate the global burden of gout and its associations with the year of diagnosis, age, geographical region, sociodemographic status and various further risk factors.MethodsRetrospective data from the Global Burden of Disease (GBD) were used, initially collected between 1990 and 2019. Raw numbers and age‐standardized rates (per 100,000 persons) of prevalence, incidence and years lived with disability (YLDs) of gout were extracted from GBD 2019 for 204 countries and territories and stratified by sex, age, year, sociodemographic index and geographic region. Correlations between gout and other chronic diseases were identified, and the burden attributable to high body mass index (BMI) and kidney dysfunction was described.ResultsThe total number of patients and gout age‐standardized prevalence rate increased between 1990 and 2019. Gout was most prevalent in Australasia and high‐income North America, and a higher sociodemographic index (SDI) was associated with higher age‐standardized prevalence, incidence and YLDs. High BMI and kidney dysfunction were risk factors for gout, while gout was correlated with other kidney diseases.ConclusionsThe global prevalence of gout, as well as incidence, and YLDs increased worldwide from 1990 to 2019 and had a significant association with sex, age, geographic region, SDI and risk factors. Understanding the complex interplay of environmental, sociodemographic and geographic risk factors is essential in mitigating the ever‐rising disease burden of gout.
Genome‐wide assessment reveals a significant association between ACSS3 and physical activity
Authors: Jo Jinyeon , Song Youngkyu , Yoon Dankyu , Lee Chung Gun , Won Sungho 
Published: December 2022

AbstractRecent genetic studies have identified physical activity (PA)‐susceptible loci in European ancestry subjects; however, due to considerable genetic differences, these findings are not likely extendable to East Asian populations. Therefore, the present study aimed to identify significantly associated PA‐susceptible loci using genome‐wide association studies (GWASs) with East Asian (EAS) subjects and to generalize the findings to European (EUR) ancestries. The mRNA levels of genes located near the genome‐wide significantly associated single‐nucleotide polymorphisms (SNP) were compared under PA and control conditions. Rs74937256, located in ACSS3 (chromosome 12), which primarily functions in skeletal muscle tissues, was identified as a genome‐wide significant variant (P = 6.06 × 10−9) in EAS. Additionally, the rs2525840, also in ACSS3 satisfied the Bonferroni corrected significance (P = 3.77 × 10−5) in EUR. We found that rs74937256 is an expressed trait locus of ACSS3 (P = 10−4), and ACSS3 mRNA expression significantly differs after PA, based on PrediXcan (P = 7 × 10−8) and the gene expression omnibus database (P = 0.043).
HomeRange: A global database of mammalian home ranges
Authors: Broekman Maarten Jaap Erik , Hoeks Selwyn , Freriks Rosa , Langendoen Merel M. , Runge Katharina M. , Savenco Ecaterina , ter Harmsel Ruben , Huijbregts Mark A. J. , Tucker Marlee A. 
Published: December 2022

AbstractMotivationHome range is a common measure of use of space by animals because it provides ecological information that is useful for conservation applications. In macroecological studies, values are typically aggregated to species means to examine general patterns of use of space by animals. However, this ignores the environmental context in which the home range was estimated and does not account for intraspecific variation in home range size. In addition, the focus of macroecological studies on home ranges has historically been biased towards terrestrial mammals. The use of aggregated numbers and the terrestrial focus limit our ability to examine home‐range patterns across different environments, their variation in time and variation between different levels of organization. Here, we introduce HomeRange, a global database with 75,611 home‐range values across 960 different species of mammals, including terrestrial, aquatic and aerial species.Main types of variables containedThe dataset contains estimates of home ranges of mammals, species names, methodological information on data collection, method of home‐range estimation, period of data collection, study coordinates and name of location, in addition to species traits derived from the studies, such as body mass, life stage, reproductive status and locomotor habit.Spatial location and grainThe collected data are distributed globally. Across studies, the spatial accuracy varies, with the coarsest resolution being 1°.Time period and grainThe data represent information published between 1939 and 2022. Across studies, the temporal accuracy varies; some studies report start and end dates specific to the day, whereas for other studies only the month or year is reported.Major taxa and level of measurementMammalian species from 24 of the 27 different taxonomic orders. Home‐range estimates range from individual‐level values to population‐level averages.Software formatData are supplied as a comma‐delimited text file (.csv) and can be loaded directly into R using the “HomeRange” R package (https://github.com/SHoeks/HomeRange).
A limited defence of public health exit restrictions
Authors: Sullivan Michael 
Published: December 2022

AbstractExit restrictions are viewed with suspicion in the literature related to migration, citizenship, and human rights. The international human rights project is devoted to defending the minimal proposition that a person should be able to leave their own country, with few exceptions. Public health emergencies constitute one of the few limits on this mobility right in Article 12 of the International Covenant on Civil and Political Rights. Here, I offer a limited legal and normative defence of pandemic travel restrictions, with a focus on Australia's COVID‐19 exit control measures. I argue that public health emergency exit controls do not constitute a violation of a traveller's mobility rights, so long as they are temporary, narrowly tailored, and proportionate to the health risk posed by the traveller in question. They should be lifted when alternative measures become widely available, such as vaccination and testing requirements.
Technological changes in the era of digitalization: What do collective agreements tell us?
Authors: Montreuil Véra‐Line , Foucher Roland 
Published: December 2022

AbstractUnionized organizations are implementing more than ever technological changes to cope with an increasingly changing and highly digital environment. Despite the extensive literature on union responses to changes, there is not much evidence on how unions and employers draft provisions pertaining to technological changes in collective agreements. Therefore, this paper aims to conduct an in‐depth analysis of these provisions in over 500 collective agreements signed between 2000 and 2020. Specifically, this study focuses on office workers in two of the most important Canadian industries, namely, the healthcare and manufacturing sectors. The findings indicate that within the examined provisions, the regulation of technological change varies along a continuum that extends from no obligations to stringent obligations on the part of the employer. Moreover, the results show that these provisions have remained stable over the past two decades.
bHLH57 confers chilling tolerance and grain yield improvement in rice
Authors: Zhang Lin , Xiang Zhipan , Li Junfeng , Wang Siyao , Chen Yi , Liu Yan , Mao Dandan , Luan Sheng , Chen Liangbi 
Published: December 2022

AbstractChilling stress has become a major limiting factor that reduces crop productivity worldwide. In this study, we identified a new gene bHLH57, whose product enhances chilling tolerance in rice at diverse developmental stages. bHLH57 was mainly expressed in leaves and anthers, and its protein was targeted to the nucleus. Overexpression of bHLH57 enhanced chilling tolerance by increasing trehalose synthesis, whereas its mutants by CRISPR/Cas9‐mediated mutagenesis were more sensitive to chilling and had reduced trehalose. Meanwhile, bHLH57 may regulate ROS metabolism and CBFs/DREBs‐ dependent pathways in response to chilling stress. In addition, the overexpression of bHLH57 resulted in increased grain yield under normal and chilling conditions, however, the disruption of bHLH57 displayed decreased grain size and seed setting rate, thus reduced grain yield. Phylogenetic and nucleotide diversity analyses suggested that bHLH57 is relatively conserved in monocotyledons, and may be selected during indica populations adaptation. Taken together, we have identified a new bHLH regulator involved rice chilling tolerance and grain yield, and provide a potential target gene for improving chilling tolerance and grain yield of rice.
Covid 19: A community based nursing disaster response
Authors: Sickora Cindy , Sickora Kathryn Rose , Smith Jane Kelly , Cerecero Jennifer , Farner Cheryl , Abebe Betelihem 
Published: December 2022

AbstractNursing has been criticized for inconsistent and episodic attention to disaster response training in academic settings. The work described herein demonstrates that nursing was not only prepared for the COVID‐19 pandemic but was able to mobilize and lead a large‐scale response that benefited a university community and the larger surrounding communities and neighborhoods paying particular attention to marginalized populations. For healthcare providers outside of hospitals, it was clear that disaster response methods would need to be implemented. The authors demonstrate that nursing established an on‐the‐ground response in collaboration with other University officials and departments. Initially established for the University community, the response was moved into surrounding neighborhoods vaccinating the city's most vulnerable. The nurse led effort answered more than 25,000 Hotline telephone calls, collected more than 30,000 COVID‐19 molecular tests, and administered more than 150,000 COVID‐19 vaccines in an operation that served up to 2500 people a day for 5 months. Nurses saved thousands of lives at the height of the COVID‐19 pandemic in hospitals and in community‐based settings. The University of Texas Health Science Center San Antonio School of Nursing demonstrated the nimble nature of academic nursing and outlines a large‐scale community response to an international pandemic in the seventh‐largest United States city. The authors establish guidelines for nurses and others to follow for future events.
Melatonin improves post‐thaw sperm quality after mild testicular heat stress in rams
Authors: Shahat Abdallah M. , Thundathil Jacob C. , Kastelic John P. 
Published: December 2022

AbstractThe objective was to determine effects of slow‐release melatonin on post‐thaw sperm quality in rams exposed to mild testicular heat stress (HS; scrotal neck insulation). Twelve yearling Dorset rams were randomly and equally allocated to receive either 36 mg melatonin in 1 ml corn oil or 1 ml corn oil injected subcutaneously (SQ); 15 day later, all rams had HS for 96 h (start of HS = start of Week 0). Semen was collected before HS and once weekly from Weeks 1 to 7, extended in Steridyl CSS One Step, held at 5°C for ~3 h, loaded into 0.5 ml straws, held 5 cm above liquid nitrogen for 10 min and then plunged. Computer assisted semen analysis (CASA) was conducted on frozen–thawed sperm. There were group and week effects for total and progressive motility (p < .001), plus group and week effects and group*week interactions (p < .001) for post‐thaw total abnormalities, acrosome integrity, post‐thaw sperm DNA fragmentation index (DFI) and high mitochondrial membrane potential (HMMP). Post‐thaw sperm total and progressive motility, acrosome integrity and HMMP were higher (p < .05) in melatonin versus control groups from Weeks 1 to 7, and the melatonin group reached baseline level (pre‐heat stress) at Week 7 (75.79 ± 0.96, 65.48 ± 1.51, 75.00 ± 0.89 and 67.00 ± 1.06, respectively; mean ± SEM). Conversely, post‐thaw sperm total abnormalities and DFI were lower (p < .05) in melatonin versus control, and both reached baseline at Week 7 in the melatonin group (26.00 ± 0.57 and 5.66 ± 0.17, respectively). Coiled tails, distal midpiece reflexes, distal cytoplasmic droplets, ruffled acrosomes, bowed midpieces, pyriform heads and knobbed acrosomes were the most common abnormalities in both groups, with lower percentages in melatonin‐treated rams. Results supported our hypothesis that HS reduces post‐thaw sperm quality, and that melatonin lessens those reductions, manifested by significantly better total and progressive motility, acrosome integrity and HMMP, and fewer sperm total abnormalities and DFI.
The Role of Banks in Shaping Income Inequality: A Within‐Country Study
Authors: Coccorese Paolo , Dell'Anno Roberto 
Published: December 2022

AbstractThis paper investigates the role of banks in shaping income inequality. We extend the current literature on the banking–inequality nexus by examining a sample of 103 Italian provinces for the period 2000–2018. We find that a higher banking development decreases income inequality. From a methodological viewpoint, we aim to contribute to the current literature by both adjusting the index of income inequality for the presence of tax evasion in income data and applying different estimation approaches to control for endogeneity. Some policy implications emerge from the findings of this study.
Nuevas perspectivas del uso de la tecnología en el ámbito del contrato de seguro
Authors: Ariza Vesga Rafael Alberto 
Published: December 2022

Las nuevas tecnologías determinan diversos impactos y retos para el contrato de seguro a nivel mundial. La blockchain, smart property y smart contracts, entre otros fenómenos, traen consigo una visión novedosa de la celebración, comercialización, reclamación e indemnización en los contratos de seguro. Los aseguradores tradicionales deben, igualmente, adaptarse a nuevas exigencias de servicio, eficiencia y celeridad, así como al surgimiento de nuevos actores que pretenden llenar las necesidades de los consumidores de seguros con mayor agilidad y menores costos. Es necesario que el legislador expida la regulación que permita el funcionamiento adecuado de estas tecnologías al interior de la actividad aseguradora en Colombia y en los demás contextos nacionales que así lo requieren.
Investigations of In-Plane Fluidelastic Instability in a Multispan U-Bend Tube Array—Part I: Tests in Air Flow
Authors: Feenstra Paul , Sawadogo Teguewinde , Smith Bruce , Janzen Victor , Cothron Helen 
Published: December 2022

Abstract
The tubes in the U-bend region of a recirculating type of nuclear steam generator are subjected to cross-flow of a two-phase mixture of steam and water. There is a concern that these tubes may experience flow-induced vibration, including the damaging effects of fluidelastic instability. This two-part series of papers presents the results of flow-induced vibration experiments performed by Canadian Nuclear Laboratories for the Electric Power Research Institute (EPRI) using the Multi-Span U-Bend test rig. The tube bundle is made of 22 U-bend tubes of 12.7 mm (0.5 in.) diameter, arranged in a rotated triangular configuration with a pitch-to-diameter ratio of 1.5. The test rig was equipped with variable clearance flat bar supports at two different locations to investigate a variety of tube and flat-bar support configurations. The primary purpose of the overall project was to study the occurrence of in-plane (or streamwise) fluidelastic instability in a U-tube bundle with flat-bar tube supports with clearances or preloads. Initially, the test rig was configured for tests in air-flow using an industrial air blower. Then tests with two-phase refrigerant (R-134a) were performed. Part I of this two-part series describes the test rig, experimental setup and some of the challenges encountered, and the results of experiments with air flows. Part II will present results of tests using refrigerant two-phase flows.
Encontro na Avenida Professor Lúcio Martins Rodrigues, 443 – Auditório da Escola de Comunicação e Artes, Universidade de São Paulo, Cidade de São Paulo, Brasil – Latitude: 23°33’ Sul, Longitude: 46°43’ Oeste – Dia 26 de julho de 2016, 10h25 (horário de Brasília), Lua 59,5% iluminada
Authors: Fabião Eleonora 
Published: December 2022

Encontro na Avenida Professor Lúcio Martins Rodrigues, 443Auditório da Escola de Comunicação e Artes Universidade de São Paulo, Cidade de São Paulo, BrasilLatitude: 23°33’ Sul, Longitude: 46°43’ OesteDia 26 de julho de 2016, 10h25 (horário de Brasília)Lua 59,5% iluminadaLink: https://www.dropbox.com/s/2k5b6xjjv1srui7/Eleonora%20Fabi%C3%A3o%20226-07-2016%20-%20VI%20SPA.wav?dl=0