bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of Novel m6A Regulator-mediated Methylation Modification Patterns in Distinct Tumor Microenvironment Profiles to Identify and Predict Glioma Prognosis and Progression, T-cell Dysfunction, and Clinical Response to ICI Immunotherapy
Authors: Zhang Wenxiong , Zhao Binghao , Xiang Zhongtian , Wu Bo , Zhang Xiang , Feng Nan , Wei Yiping 
Published: December 2022

Background:
The specific functions of RNA N6-methyladenosine (m6A) modifications in the glioma
tumor microenvironment (TME) and glioma patient prognosis and treatment have not been determined to
date.

Objective:
The objective of the study was to determine the role of m6A modifications in glioma TME.

Methods:
Nonnegative matrix factorization (NMF) methods were used to determine m6A clusters and m6A
gene signatures based on 21 genes relating to m6A modifications. TME characteristics for each m6A cluster
and m6A gene signature were quantified by established m6A score. The utility of m6A score was validated in
immunotherapy and other antiangiogenic treatment cohorts.

Results:
Three m6A clusters were identified among 3,395 glioma samples, and they were linked to different biological
activities and clinical outcomes. The m6A clusters were highly consistent with immune profiles known
as immune-inflamed, immune-excluded, and immune-desert phenotypes. Clusters within individual tumors
could predict glioma inflammation, molecular subtypes, TME stromal activity, genetic variation, alternative
splicing, and prognosis. As for the m6A score and m6A gene signature, patients with low m6A scores exhibited
an increased tumor mutation burden, immune activity, neoantigen load, and prolonged survival. A low m6A
score indicated the potential for a low level of T-cell dysfunction, a considerably better treatment response, and
durable clinical benefits from immunotherapy, bevacizumab and regorafenib.

Conclusion:
Glioma m6A clusters and gene signatures have distinctive TME features. The m6A gene signature
may guide prognostic assessments and promote the use of effective strategies.
High-Intense Interval Training Prevents Cognitive Impairment and
Increases the Expression of Muscle Genes FNDC5 and PPARGC1A in a
Rat Model of Alzheimer's Disease
Authors: Nogueira Godinho Welton Daniel , Vasconcelos Filho Francisco Sérgio Lopes , Pinto Daniel Vieira , Alves Juliana Osório , de Souza Nascimento Tyciane , de Aguiar Isabele Dutra , Silva Almeida Guilherme Nizan , Ceccatto Vânia Marilande , Soares Paula Matias 
Published: December 2022

Background:
Alzheimer's disease is the most common neurodegenerative disease in the world, characterized by the progressive loss of neuronal structure and function, whose main histopathological landmark is the accumulation of β-amyloid in the brain.

Objective:
It is well known that exercise is a neuroprotective factor and that muscles produce and release myokines that exert endocrine effects in inflammation and metabolic dysfunction. Thus, this work intends to establish the relationship between the benefits of exercise through the chronic training of HIIT on cognitive damage induced by the Alzheimer's model by the injection of β amyloid 1-42.

Methods:
For this purpose, forty-eight male Wistar rats were divided into four groups: Sedentary Sham (SS), Trained Sham (ST), Sedentary Alzheimer’s (AS), and Trained Alzheimer’s (AT). Animals were submitted to stereotactic surgery and received a hippocampal injection of Aβ1-42 or a saline solution. Seven days after surgery, twelve days of treadmill adaptation followed by five maximal running tests (MRT) and fifty-five days of HIIT, rats underwent the Morris water maze test. The animals were then euthanized, and their gastrocnemius muscle tissue was extracted to analyze the Fibronectin type III domain containing 5 (FNDC5), PPARG Coactivator 1 Alpha (PPARGC1A), and Integrin subunit beta 5 (ITGB5-R) expression by qRT-PCR in addition to cross-sectional areas.

Results:
The HIIT prevents the cognitive deficit induced by the infusion of amyloid β 1-42 (p<0.0001), causes adaptation of muscle fibers (p<0.0001), modulates the gene expression of FNDC5 (p<0.01), ITGB5 (p<0.01) and PPARGC1A (p<0.01), and induces an increase in peripheral protein expression of FNDC5 (p<0.005).

Conclusion:
Thus, we conclude that HIIT can prevent cognitive damage induced by the infusion of Aβ1-42, constituting a non-pharmacological tool that modulates important genetic and protein pathways.
Identification of Potential Inhibitors of PDE5 based on Structure-based
Virtual Screening Approaches
Authors: Xu Lei , Sun Lilei , Su Peng , Ma Teng , Yu Yingcong , Liu Haibin , Huang Xianfeng 
Published: December 2022

Background:
Phosphodiesterase type 5 (PDE5), exclusively specific for cyclic guanidine
monophosphate (cGMP), a potential target for the therapy of various diseases, and PDE5 inhibitors
could be used as a treatment for erectile dysfunction (ED) or chronic pulmonary hypertension.

Objective:
In the present study, we carried out an integrated computer-aided virtual screening
technique against the natural products in the ZINC database to discover potential inhibitors of
PDE5.

Methods:
Pharmacophore, molecular docking and ADMET (Absorption, distribution, metabolism,
excretion and toxicity) properties filtration were used to select the PDE5 inhibitors with the best
binding affinities and drug-like properties. The binding modes of PDE5 inhibitors were investigated,
and these complexes' stabilities were explored by molecular dynamic simulations and
MM/GBSA free energy calculations.

Results:
Two natural compounds (Z171 and Z283) were identified and may be used as a critical
starting point for the development of novel PDE5 inhibitors. The MM/GBSA free energy decomposition
analysis quantitatively analyzed the importance of hydrophobic interaction in PDE5-
ligands binding.

Conclusion:
In this study, we identified two novel natural compounds from the ZINC database to
effectively inhibit PDE5 through virtual screening. The novel scaffolds of these compounds can be
used as the starting templates in the drug design of PDE5 inhibitors with good pharmacokinetic
profiles. These results may promote the de novo design of new compounds against PDE5.
An Overview of Radiolabeled RGD Peptides for Theranostic Applications
Authors: Badipa Fateme , Alirezapour Behrouz , Yousefnia Hassan 
Published: December 2022

Abstract:
Angiogenesis phenomenon, as a highly affecting factor on the growth and spread of
cancer cells, depends on specific molecular interactions between components of the extracellular
matrix and vascular cells. αv integrin acts as a cell adhesive molecule involved in tumor invasion
and angiogenesis. Among the various combinations of integrin subunits expressed on the surface
of cells, αvβ3 integrin has a particularly interesting expression pattern during angiogenesis. The
αvβ3 integrin is a vital receptor affecting tumor growth, tumor invasiveness, metastasis, and angiogenesis
overexpressed on various human tumors, leading to the development of different
theranostics probes and radiopharmaceuticals. The αvβ3 integrin can recognize several extracellular
matrix molecules in the base of the RGD adhesive sequence. This review provides an overview
of the status, trends and future of the most studied αvβ3 integrin-binding ligand, RGD tripeptides,
labeled with various radioisotopes. An overview of the pre-clinical models for radiolabeled RGD
peptides and clinical aspects of the RGD- based radiopharmaceuticals is provided with some new
considerations and ways forward.
Emergence of BA.4/BA.5 Omicron Sub-lineages and Increased SARSCoV-
2 Incidence in Senegal
Authors: Diagne Moussa Moise , Diallo Amadou , Sankhe Safietou , Top Ndeye Marieme , Kane Mouhamed , Mbanne Maimouna , Faye Martin , Barry Mamadou Aliou , Dia Ndongo , Sall Amadou Alpha , Loucoubar Cheikh , Faye Ousmane 
Published: December 2022

Background:
The Omicron variant B.1.1.529 has led to a new dynamic in the COVID-19 pandemic, with an increase in cases worldwide. Its rapid propagation favors the emergence of novel sub-lineages, including BA.4 and BA.5. The latter has shown increased transmissibility compared to other Omicron sub-lineages. In Senegal, the emergence of the Omicron variant in December 2021 characterized the triggering of a short and dense epidemiological wave that peaked at the end of February. This wave was followed by a period with a significant drop in the number of COVID-19 cases, but an upsurge in SARS-CoV-2 infection has been noted since mid-June.

Objective:
The purpose of this brief report is to give an update regarding the genomic situation of SARS-CoV-2 in Dakar during this phase of recrudescence of cases.

Methods:
We performed amplicon-based SARS-CoV-2 sequencing on nasopharyngeal swab samples from declared COVID-19 patients and outbound travelers that tested positive.

Results:
Ongoing genomic surveillance activities showed that more than half of recent COVID-19 cases were due to the BA.4 and BA.5 sub-lineages that share two critical mutations associated with increased transmissibility and immune response escape. The circulation of recombinants between Omicron sub-lineages was also noted.

Conclusion:
Despite the lack of proven severity of BA.4 and BA.5 sub-lineages, their increased transmissibility causes a rapid spread of the virus, hence a surge in the number of cases. This rapid spread constitutes a greater risk of exposure for vulnerable patients. To tackle this issue, any increase in the number of cases must be monitored to support public health stakeholders. Therefore, genomic surveillance is an ever-essential element in managing this pandemic.
IDENTIFIKASI Escherichia coli PADA AIR MINUM ISI ULANG
Authors: Sulistyani Nunung 
Published: December 2022

Air memiliki peran penting bagi setiap makhluk hidup untuk memenuhi kebutuhan esensial. Asupan cairan dalam hal ini air minum menjadi sangat penting bagi tubuh manusia, maka perlu diperhatikan kualitas air minum sesuai standar yang telah ditetapkan. Saat ini, banyak penyelenggara air minum isi ulang mendapatkan ijin operasi untuk memproduksi air minum bagi masyarakat. Untuk menjaga kualitas air minum yang dikonsumsi masyarakat, dilakukan pengawasan secara berkala baik secara internal maupun eksternal. Uji mikrobiologis dalam penelitian ini dilakukan pada air minum isi ulang “KHAIRA” Yogyakarta yang memberikan pelayanan air minum isi ulang dengan kualitas terjamin yang telah mendapatkan sertifikat laik higienis dari Dinas Kesehatan Kota Yogyakarta. Untuk itu perlu dilakukan pengawasan eksternal melalui penelitian ini, sehingga dapat dilakukan pengkajian kualitas air minum isi ulang Khaira melalui uji mikrobiologis. Penelitian ini dilakukan dengan melakukan identifikasi Escherichia coli pada air minum isi ulang. Data diperoleh melalui pemeriksaan air isi ulang menggunakan metode Most Probable Number (MPN). Hasil uji MPN menunjukkan tidak terdeteksi Escherichia coli pada semua sampel air. Kualitas air minum isi ulang yang diproduksi “Khaira” Yogyakarta berdasarkan uji identifikasi Escherichia coli memenuhi persyaratan batas cemaran mikroba
Evaluation of OPTIMISE (Online Programme to Tackle Individual’s Meat Intake Through Self-regulation): Cohort Study
Authors: Stewart Cristina , Piernas Carmen , Frie Kerstin , Cook Brian , Jebb Susan A 
Published: December 2022

Background
There is an urgent need to reduce society’s meat consumption to help mitigate climate change and reduce noncommunicable diseases.

Objective
This study aimed to investigate changes in meat intake after participation in an online, multicomponent, self-regulation intervention.

Methods
We conducted a pre-post observational study among adult meat eaters in the United Kingdom who signed up to a website offering support based on self-regulation theory to reduce meat consumption. The program lasted 9 weeks (including a 1-week baseline phase, a 4-week active intervention phase, and a 4-week maintenance phase), comprising self-monitoring, goal setting, action planning, and health and environmental feedback. Meat intake was estimated during weeks 1, 5, and 9 using a 7-day meat frequency questionnaire. We analyzed the change in mean daily meat intake from baseline to week 5 and week 9 among those reporting data using a hierarchical linear mixed model. We assessed changes in attitudes toward meat consumption by questionnaire and considered the acceptability and feasibility of the intervention.

Results
The baseline cohort consisted of 289 participants, of whom 77 were analyzed at week 5 (26.6% of the baseline sample) and 55 at week 9 (71.4% of the week 5 sample). We observed large reductions in meat intake at 5 and 9 weeks: –57 (95% CI –70 to –43) g/day (P<.001) and –49 (95% CI –64 to –34) g/day (P<.001), respectively. Participants’ meat-free self-efficacy increased, meat-eating identities moved toward reduced-meat and non–meat-eating identities, and perceptions of meat consumption as the social norm reduced. Participants who completed the study reported high engagement and satisfaction with the intervention.

Conclusions
Among people motivated to engage, this online self-regulation program may lead to large reductions in meat intake for more than 2 months, with promising signs of a change in meat-eating identity toward more plant-based diets. This digital behavior change intervention could be offered to complement population-level interventions to support reduction of meat consumption.
Formulation and Evaluation of Mucoadhesive Bilayered Buccal Tablet of Ziprasidone Hydrochloride
Authors: Omer Mohammed , Shahidulla S.M. 
Published: December 2022

The present project was carried out to formulate and evaluate the mucoadhesive bilayered buccal tablet of Ziprasidone Hcl. Ziprasidone HCl is an antipsychotic agent with half-life of about 2hrs and shows extensive metabolism i.e the drug concentration is < 5% after elimination in the body. The formulations (F1 to F6) were developed which comprises of polymers such as Hydroxypropyl methyl cellulose (HPMC K-15), Polyvinyl pyrollidine (PVP K-30) in various concentrations along with carbopol to achieve the desired characteristics. Mucoadhesive bilayered buccal tablets were fabricated with the aid of direct compression technique and the prepared formulation was evaluated for its physicochemical parameters along with evalauation test. The results from different evaluation test demonstrated that the formulation F1 containing HPMC (25 mg) and CP (10 mg) was selected as optimised formulation and result values of precompression parameters were within the limits and post compression results showed the mucoadhesive strength of F1 formulation was 25.27gm, the drug release at 8th hr was 85.7 % and the formulation was stable throughout the stability studies. Hence mucoadhesive bilayered buccal tablets of Ziprasidone HCl can be prepared. Based on the above results it can be stated that mucoadhesive bilayered buccal tablets can be successfully developed. Formulation described that the nature of tablet depends not only on the se lected polymer excipient but also on the concentration of polymers selected.
Keywords: Ziprasidone HCl, Bilayered Buccal Tablet, Mucoadhesion.
A Review on Ayurvedic Cosmeceuticals and their Mode of Actions
Authors: Hirudkar Vandana N. , Shivhare Vishal 
Published: December 2022

The uses of herbal ingredients in cosmetic industry increases day by day since these products improves natural beauty and also gives anti-aging effect. The presence of chemicals such as essential oils, alkaloids, proteins, flavonoids, vitamins and minerals, etc., contributed towards the cosmeceutical properties of herbal cosmetics. Ayurveda described uses of cosmetic for retaining health of skin, hair and tooth, etc. The Ayurveda cosmeceutical provides Vayasthapana, Varnya, Sandhaniya, Tvachya, Shothahara and Tvachagnivardhani effects thus helps to retain beauty and resist symptoms of early aging. Natural products such as Amla, Gotukola, Neem, Henna, Bael, Brahmi, Haritaki, Shikakai, Bhringaraj and Reetha, etc. mainly used for the beautification purpose. Some Ayurveda formulations or topical preparations and oils also recommended for improving health of skin and hair. Ayurveda cosmeceuticals offers advantages of availability, safety and free from unwanted chemical reactions. Present article summarizes the key aspects related to the natural cosmeceuticals and their mode of action.
Keywords: Ayurveda, Beauty, Cosmeceuticals, Vayasthapana, Tvachya
Availability of Drugs for Mothers and Children in Community Health Centers in Commune VI of the Bamako Health District
Authors: TRAORE Sylvestre , COULIBALY Issa , TRAORE Mohamed dit Sarmoye , MALLE Fatoumata S , BAH Sekou 
Published: December 2022

Introduction: Improving maternal and child health is a worldwide priority. An estimated 8.1 million children under five die each year. Approximately 1,000 women, most of whom live in developing countries, die every day from complications of pregnancy or childbirth. The objective of this work was to assess the availability of mother's and child's medicines at the level of the community health center VI of the Bamako district, Mali.
Materials and methods: This were a prospective cross-sectional study which was took place in Bamako in the community health center VI in 12 months over the period from November 2019 to October 2020. We carried out a survey in the 11 community health center VI. Data entry and analysis were performed using SPSS version 21.0 software.
Results: In this study, the sex ratio was 2.66. Half of the people surveyed were doctors (50%) who assumed the role of DTC (Technical Director of the Center). As for the profiles of managers, in our sample, accountants were the most represented with a rate of 31.82%. Almost all depot managers have received training in logistics management (86% of cases). 73.00% of Community health centers correctly followed the SDADME (drug supply and distribution master plan). The Community health centers were supplied by other suppliers in the event of a shortage at the PPM (Popular Pharmacy of Mali) with a rate of 86.40%. The total availability rate of medicines for mothers and children was 60%. Breaks were recorded for eight (08) products. The causes mentioned in relation to drug breaks were, among others: non-ordering of drugs, non-availability at the PPM and non-prescription of drugs by providers.
Conclusion: This study revealed a good availability of drugs for mother and child. However, we found that some of these drugs were out of stock. These shortages were due either to the non-availability of the PPM store, or to the non-ordering of these products by the Community health centers.
Keywords: management, medicine, CSComs, mothers and children, Bamako.
C-Reactive Protein (CRP) Medium and Severe Symptoms Levels of COVID-19
Authors: Manalu Erida , Winanda Adelia , Luhulima Danny Ernest Jonas 
Published: December 2022

Coronavirus Disease 2019 (COVID-19) is a disease caused by SARS-CoV-2 and has caused a global pandemic. Patients with COVID-19 can experience symptoms such as fever, dry cough, headache, and shortness of breath which can lead to pneumonia. Excessive inflammation is considered a leading cause of critical illness and death in COVID-19 patients. C-Reactive Protein is a sensitive indicator as an early marker of infection and inflammation. This study presents an overview of the difference in the average CRP levels between patients with moderate and severe confirmed COVID-19 symptoms at UKI Hospital for June-September 2021. The study design was cross-sectional with descriptive and analytical data presentation obtained from medical record documents at UKI Hospital. The average CRP level of COVID-19 patients with moderate symptoms is 63,705 mg/L, totaling 139 patients. The average CRP level of COVID-19 patients with severe symptoms is 132,050 mg/L, totaling 57 patients. The results obtained from the Mann-Whitney test between groups of patients with moderate and severe COVID-19 showed a significant difference between CRP levels with moderate and severe symptoms with p <0.05.
Keywords: COVID-19, CRP levels, SARS-CoV-2
Contribution to the ethnobotanical and phytochemical study of Zanthoxylum zanthoxyloides (Lam.) Waterman (Rutaceae) from Mali
Authors: Cissé Salimatou , Somboro Aimé Anin , Cissé Mody , Bouaré Sékou , Tangara Ousmane , Dembélé Niaboula , Samaké Drissa , Togola Issiaka 
Published: December 2022

Zanthoxylum zanthoxyloides (Lam.) Waterman (Rutaceae) is a tree from 5 to 15 m high that grows spontaneously in West Africa. It is frequently used in Malian traditional medicine for the treatment of various ailments. Thus, the present work is part of the development of this plant through an ethnobotanical survey and a phytochemical study of its leaves, stems and roots collected in Yanfolila and Ouélessébougou. The ethnobotanical survey showed that this species is used in the treatment of various pathologies such as tooth decay, toothache, throat, stomach and headaches, sickle cell anemia, sterility, cardiovascular diseases, edema, childbirth pains, and other diseases, sexual impotence, malaria, bilharzia, diabetes, hemorrhoids, dermatosis, constipation, fever, eye infections, dysentery. In most cases the preparation is done by decoction. The phytochemical screening showed the presence of coumarins, sterols and terpenes in the three investigated organs from the two sites. The alkaloids have been found in the roots and in the stems; the mucilages in all parts except in the roots of Yanfolila. The tannins are present everywhere except in the roots and in the stems of Yanfolila; flavonoids are present everywhere except in the stems of Yanfolila. The richness of the powder of these different organs in mineral elements such as calcium, iron, magnesium, sodium, potassium and copper was also highlighted.
Keywords: Zanthoxylum zanthoxyloides, ethnobotanical survey, phytochemistry, mineral elements
Fee for vehicle parking spaces: definition of the tax and legal concept
Authors: Nedostup К. , Iliushchenko Н. 
Published: December 2022

The article contains the overview of the results of the scientific study of the interpretation of the tax and legal concept of "fee for vehicle parking spaces".
The legal acts that regulate the tax legal relations in the field of ​​collection of local fees were developed. It was established that the mandatory tax payments that are belong exclusively to the field of parking services, have existed in the domestic tax system since May 20,1993 and until now. The transformation of their name was presented.The facts were revealed when: 1) the same mandatory tax payment at the same time has the different legalized name; 2) the different names of the local fee were used in different articles of the legal act.It was emphasized that the tax and legal concepts of "parking fee", "vehicle parking fee" and "fee for vehicle parking spaces" did not have the legal definition. It was analyzed the definitions of the concepts of "fee for vehicle parking spaces" which were formulated by modern scientists. With the help of semantic analysis of the name of the mentioned local fee, the existing tautology in it was revealed. It was proposed to exclude the words "vehicles" or "parking fee" from it. It was noted that it is possible not to emphasize the object of taxation in the name of the fee. Then, it will be such as "parking fee".
The definition of the concept of "fee for parking spaces" was formulated. It is the mandatory payment that is made to the local budget from the legal entities and natural persons - entrepreneurs who provide the parking services at the specially designated site and / or parking lot, communal property; the type of local fee. Such definition contains the most essential features specific to this local fee and characterizes it in relation to the tax system and the budget, highlights the composition of fee payers and the circumstances under which the latter have the tax obligation.