bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-agent Approach for Vehicle-to-Infrastructure Routing in VANETs: A Machine Learning Framework
Authors: Sataraddi Mamata J. , Kakkasageri Mahabaleshwar S. , Bandi Ajay , Pujar Rajani S. 
Published: June 2022

Abstract
The vehicular network plays a significant role in understanding the detailed study of vehicle communications. Multiple vehicles in the local communication range need to exchange the safety and infotainment information via common roadside infrastructure in Vehicular Ad hoc Networks (VANETs). The vehicle-to-Infrastructure (V2I) communication model helps to improve the efficiency of the intelligent transport system by providing safety warnings and reducing vehicle collisions. Machine learning is an artificial intelligence component that allows the machine to learn without being expressly trained to improve from experience automatically. Since VANET is imprecise and uncertain, Machine Learning (ML) and Software Agents (SAs) combining approaches resolve the issues of V2I communication challenges in VANETs. This paper proposes ML-based V2I Communication in VANETs using a software agent approach. The proposed agent-based model is made up of both static and mobile agents. The proposed model executes the decision tree algorithm to identify the event as non-critical or critical. The Q-Learning algorithm identifies the destination vehicle with improved bandwidth utilization, packet delivery ratio, end-to-end delay, V2I communication delay, and throughput and control overheads.
COVID-19 risk perception framework of the public: An infodemic tool for future pandemics and epidemics
Authors: Lohiniva Anna-Leena , Pensola Annika , Hyökki Suvi , Sivelä Jonas , Tammi Tuukka 
Published: June 2022

Abstract
Understanding risk perceptions of the public is central for risk communications and infodemic management during emergency and preparedness planning as people’s behavior depends on how they perceive the related risks. This qualitative study aimed to identify and describe factors related to COVID-19 risk perceptions of the public in Finland and to make this information readily available for those who communicate with the public during crises. The study is part of a larger project exploring crisis narratives through a mixed-methods approach. The study was based on a dataset of over 10,000 comments on the Facebook and Twitter posts of the Finnish Institute of Health and Welfare (THL) between March -May 2021. The data was analyzed qualitatively using thematic analysis. The study identified concepts linked with the pandemic risk perception that included knowledge, perceptions, personal experiences, trust, attitudes, and cultural values. The findings resulted in a framework of risk perceptions that can be used as taxonomy and a set of keywords to be used in social listening to monitor risk perception during future epidemics and pandemics.
Epidermal stem cell-derived exosomes alleviate autophagy induced endothelial cell apoptosis through miR200b-3p/ERK pathway in diabetic wounds
Authors: Xu hailin , Wang Zhiyong , Yang Hao , Chen Chufen , Dong Yunxian , Xu Zhongye , Lv Dongming , Wang Peng , Yuan Mingzhou , Wang Xiaoyan , Chen Miao , Tang Bing , Hu Zhicheng , Deng Wuguo , Zhu Jiayuan 
Published: June 2022

Abstract
Backgrounds: Diabetic wounds have the characteristics of abnormal inflammatory response, decreased neovascularization, and disturbed microenvironment. Clinical use of epidermal basal cell suspensions promotes chronic wound healing. Further study found that rat Epidermal stem cells (EpiSCs) could promote the angiogenesis of full-thickness wounds in SD rats. The mechanism of the effect of EpiSCs on wound healing warrants further study.Methods: In vivo, EpiSCs were labeled with enhanced green fluorescent protein and then sprayed on dorsal wound of db/db mice. The quality of wound healing was estimated by the residual wound area rate and Hematoxylin and Eosin (H&E) staining. Expression of neovascular markers and collagen formation were elucidated by immunohistochemistry (IHC), immunofluorescence (IF), and MASSOM.In vitro, the supernatant of EpiSCs were ultra-centrifugated to collected exosomes (EpiSC-EXOs). Human umbilical vein endothelial cells (HUVECs) were treated with high glucose and EpiSC-EXOs. miR 200b-3p is identified abundant in EpiSC-EXOs using RNA sequencing. HUVECs were analyzed with apoptosis rate, ROS level, proliferation and mitochondrial membrane potential.Results: In vivo, Epidermal stem cells (EpiSCs) could colonize in wounds, promoting angiogenesis thus accelerating wound healing in diabetic mouse model (db/db mouse). In vitro, EpiSC-EXOs had the ability to reduce apoptosis by downregulating excessive autophagy induced by high-glucose conditions. Furthermore, we found that miR200b-3p rich in EpiSC-EXOs affected the phosphorylation of ERK by targeting SYDE1 to regulate intracellular autophagy levels, thereby reducing vascular endothelial cell apoptosis caused by a high-glucose environment.Conclusions: Collectively, our results verified the effect of EpiSC-EXOs on apoptosis caused by a high-glucose environment in vascular endothelial cells and revealed that its underlying mechanism occurs through the miR200b-3p/SYDE1/RAS/ERK pathway. Our study explains the role of EpiSCs in promoting wound healing in diabetic patients.
Predicting IVF outcome in poor ovarian responders
Authors: Lebovitz Oshrit , Haas Jigal , Mor Nitzan , Zilberberg Eran , Aizer Adva , Kirshenbaum Michal , Orvieto Raoul , Nahum Ravit 
Published: June 2022

Abstract
Background
Poor responders to ovarian stimulation are one of the most challenging populations to treat. As a failed cycle can cause a considerable emotional and economical loss, adequate fertility counseling addressing patients’ expectations are highly important when facing patients with poor ovarian response. The study aimed to evaluate reproductive outcomes and to identify factors associated with live birth (LB) after fresh autologous IVF/ intracytoplasmic sperm injection (ICSI) cycles of patients fulfilling the Bologna criteria for poor ovarian response (POR).
Methods
A retrospective study included 751 IVF/ICSI treatment cycles which yielded up to three retrieved oocytes, at a tertiary referral hospital between January 2016 to February 2020. A logistic regression analysis was used to adjust for confounders.
Results
Clinical pregnancy and LB rate per cycle were significantly higher among women younger versus older than 40 years (9.8% and 6.8% versus 4.5% and 2.1%, p < 0.01, respectively). Patients who achieved LB were significantly younger, had higher number of oocytes retrieved, fertilization rate and top-quality embryos (P < 0.05). Multivariable regression analysis identified patient’s age (OR 0.90; 95% CI 0.845 to 0.97; p = 0.005) and mean number retrieved oocytes (OR 1.95; 95% CI 1.20 to 3.16; p = 0.007) as factors significantly associated with the probability of a LB.
Conclusions
The woman’s age and the number of retrieved oocytes are both independent predicting factors of live birth in poor ovarian responders. Considering the risks, the high financial investment and poor reproductive outcomes involved in IVF treatments, raises questions regarding the adequacy of providing treatments in these patients' population. POR younger than 40 years may represent a possible exception due to acceptable probability for a LB.
Influence of two supervised physical exercise programs on quality of life and depressive symptomatology: comparative analysis in three companies with sedentary occupations
Authors: Remón Ángel Luis Clemente , Díaz-Benito Víctor Jiménez , Díaz Sara Zafra , Jiménez-Beatty José Emilio , Moro María Isabel Barriopedro , Mateos María Espada , Lozano José Antonio Santacruz 
Published: June 2022

Abstract
The purpose of this paper is to assess the effect of two supervised physical exercise interventions in the workplace on quality of life and depressive symptomatology. A pre-experimental study was conducted on workers with sedentary occupations from three different companies and sectors: (1) Renewable energy engineering; (2) Insurance and (3) Infrastructure and services. The participants from the first two were administered a supervised physical exercise program in three Spanish corporate headquarters: Madrid, Navarre, the Basque Country and Madrid, respectively (PRODET→; n = 23; mean age 44.38 ± 4.91 years). The third company carried out another physical exercise intervention in a single corporate headquarters in Madrid (HASAVI; n = 27; mean age 39.25 ± 9.83). A pre- and post-test intragroup analysis of quality of life and depressive symptomatology was performed. The PRODETⓇ program found a reduction in body pain in workers at the Madrid headquarters of the renewable engineering company (p = 0.024; η2 = 0.18). In this same company, improvements in global physical health scores were found in the Navarre headquarters (p = 0.009; η2 = 0.40), as well as in the insurance company (p = 0.048; η2 = 0.10). An increase in general health was found in the Basque Country headquarters (p = 0.034; η2 = 0.33). The HASAVI program achieved a significant improvement in vitality (p = 0.041; η2 = 0.09). Both programs found a significant reduction in depressive symptoms in workers in the insurance and infrastructure and services company (p = 0.044; η2 = 0.12; p = 0.045; η2 = 0.10, respectively). The PRODETⓇ supervised physical exercise program could be effective in improving the quality of life of office employees in the sectors studied. Both programs could influence the decrease in depressive symptomatology.
Two circPPFIA1s negatively regulate liver metastasis of colon cancer via miR-155- 5p/CDX1 and HuR/RAB36
Authors: Ji Haein , Kim Tae Won , Lee Woo Joo , Jeong Seong Dong , Cho Yong Beom , Kim Hyeon Ho 
Published: June 2022

Abstract
Background
Circular RNAs (circRNAs) play a critical role in colorectal cancer (CRC) progression, including metastasis. However, the detailed molecular mechanism is not fully understood.
Methods
Differentially expressed circRNAs between primary KM12C and liver metastatic KM12L4 colon cancer cells were identified by microarray. The expression of circRNAs was measured by semi-quantitative (semi-qPCR) and real time-quantitative PCR (RT-qPCR). Metastatic potential including invasive and migratory abilities, and liver metastasis were examined by transwell assays and intrasplenic injection, respectively. CircPPFIA1-associated microRNA (miRNA) and RNA-binding protein (RBP) were screened by an antisense oligonucleotide (ASO) pulldown experiment. The effects of circPPFIA1 on target gene expression were evaluated by RT-qPCR and western blot analyses.
Results
By analyzing circRNA microarray data, we identified two anti-metastatic circRNAs generated from PPFIA1 with different length, which named circPPFIA1-L (long) and -S (short). They were significantly downregulated in liver metastatic KM12L4 cells compared to primary KM12C cells. The knockdown of circPPFIA1s in KM12C enhanced metastatic potential and increased liver metastasis. Conversely, overexpression of circPPFIA1s weakened metastatic potential and inhibited liver metastasis. circPPFIA1s were found to function as sponges of oncogenic miR-155-5p and Hu antigen R (HuR) by an ASO pulldown experiment. circPPFIA1s upregulated tumor-suppressing CDX1 expression and conversely downregulated oncogenic RAB36 by decoying miR-155-5p and by sequestering HuR, respectively.
Conclusions
Our findings demonstrate that circPPFIA1s inhibit the liver metastasis of CRC via the miR-155-5p/CDX1 and HuR/RAB36 pathways.
Quality of Life in patients diagnosed with Filum Disease and Neuro-cranio-vertebral Syndrome with Arnold-Chiari Syndrome Type I, Idiopathic Syringomyelia and Idiopathic Scoliosis
Authors: Royo-Salvador Miguel B. , Luè Gioia 
Published: June 2022

Abstract
BackgroundFilum Disease and Neuro-cranio-vertebral syndrome are two recently described pathological concepts that comprise various diseases including Arnold-Chiari syndrome type I, idiopathic syringomyelia and idiopathic scoliosis. The objectives of this article are to describe the neuro-psychological alterations in Filum disease and Neuro-cranio-vertebral syndrome, and to carry out a descriptive analysis of how these diagnoses can influence Quality of Life and/or perception of it.MethodsWe conducted a retrospective study of the clinical anamneses of patients diagnosed with Filum Disease from a sample of 372 randomly selected patients (Sample 1) in relation to their Quality of Life. We also analysed the self-reported psychological-emotional health in 34 other patients (Sample 2). Only descriptive statistics were carried out for both samples.ResultsDespite the high frequency of pain and neurological symptoms, we detected that mood alterations and cognitive and sexual dysfunction may also be affected in Filum Disease and Neuro-cranio-vertebral syndrome. Filum Disease deteriorates Quality of Life and likely also affects patients´ perceived Quality of Life. Many patients report how the gradual and degenerative worsening of symptoms in turn progressively affects their self-assessment and self-perception of their own physical and psychological health, consequently influencing their expectations in various areas of life.ConclusionWe have shown that the Quality of Life in patients with Filum disease can be affected by the limited physical condition, mainly due to headaches or symptoms of spinal or limb pain, instability, lack of sensitivity or strength, or paresis. The consequences that Filum Disease can have on environmental and social context correspond to a further decrease in Quality of Life. For the diagnosis, prognosis, surgical treatment, and post-operative evaluation, it is essential to consider everything reported in the initial anamnesis. Otherwise, the results and effectiveness could be affected.
A new in vitro device reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage
Authors: Barrit Thibault , Campion Claire , Aligon Sophie , Bourbeillon Julie , Rousseau David , Planchet Elisabeth , Teulat Béatrice 
Published: June 2022

Abstract
Background: Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in plant-pathogen interactions, its role has never been studied during this early phase for the interaction between Arabidopsis thaliana and Alternaria brassicicola, a seed-transmitted necrotrophic fungus. The aim of the present work was to develop an in vitro device allowing to study the impact of the fungus on A. thaliana seedling growth, while modulating N nutrition.Results: The developed device consists of square plates placed vertically and filled with nutrient agar medium allowing modulation of N conditions. Seeds are inoculated after sowing by depositing a droplet of conidial suspension. A specific semi-automated image analysis pipeline based on the Ilastik software was developed to quantify the impact of the fungus on seedling aerial development, calculating an index accounting for every aspect of fungal impact, namely seedling death, necrosis and developmental delay. The device also permits to monitor root elongation. The interest of the device was then confirmed by characterising how N media composition (0.1 and 5 mM of nitrate (NO3-), 5 mM of ammonium (NH4+)) affects the impact of the fungus on three A. thaliana ecotypes. Seedling development was strongly and negatively affected by the fungus. However, seedlings grown with 5 mM NO3- were less susceptible than those grown with NH4+ or 0.1 mM NO3-, which differed from what was observed with adult plants (rosette stage).Conclusions: The developed device allows accurate determination of seedling growth characteristics (both on aerial and root parts) and symptoms. Altogether, the device could be used to study the impact of plant nutrition on susceptibility of various genotypes to fungi at the seedling stage.
Determination of EDTA-2Na in pickles and canned mix congee by a fluorescent Zirconium-Quercetin complex
Authors: Jin Di , Wang Can , Yue Xiaoqing , Wang Jun 
Published: June 2022

Abstract
In this study, we developed and validated a three-dimensional fluorescence method for the determination of Ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) in pickles and canned mix congee by forming a ternary fluorescent complex of zirconium-quercetin-EDTA system. The conditions of complexation reaction and fluorescence scanning parameters were optimized. During the fluorescent complex reaction, NH3·H2O and anhydrous ethanol were used to enhance the fluorescence intensity. The analysis of EDTA-2Na by fluorescence scanning could be accomplished within 3 minutes. Under the condition, satisfactory linearities were obtained (R2≥0.99) with the limits of quantitation (LOQs) of 24.6 µg/g. The recoveries were 79.8-113.2%, with a relative standard deviation (RSD) below 9.7%. Compared with the HPLC-DAD method, the relative error of the two methods was less than 10%, which indicated the results of the two methods were consistent. A convenient and accurate method was established to analyze EDTA-2Na in pickles and canned mix congee, which provided a new option to rapidly monitor EDTA-2Na in foods.
CD44 as a prognostic index of brain cancers and therapeutic target of cold atmospheric plasma
Authors: Cai Linhan , Chen Yiming , Dai Xiaofeng 
Published: June 2022

Abstract
IntroductionBrain malignancies, including both primary and metastatic brain cancers, constitutes as one significant factor to cancer-associated mortality and poor quality-of-life of patients that remains as a global challenge in cancer research and clinical management. This study aims to identify potential biomarkers of brain cancers through pan-cancer analysis and explore potential novel therapeutic approaches. Material and methodsWe downloaded and analyzed 10 GEO datasets that satisfy our selection criterion through differentially expressed gene identification, Pearson correlation analysis, protein-protein interaction network construction, KEGG pathway and Gene Ontology term enrichment, and survival analysis. We identified independent prognostic markers on brain cancer overall survival and constructed the unified index with improved prediction power on brain cancer clinical outcome. We experimentally investigated the functionalities of the identified key marker on brain cancer cell proliferation, migration and cancer stemness, and explored the feasibility of cold atmospheric plasma in arresting brain cancer cells via targeting this identified marker.ResultsWe identified the critical role of CD44 in characterizing brain cancer malignancy, constructed a unified index involving CD44 for brain cancer prognosis, and demonstrated the efficacy of cold atmospheric plasma in arresting brain cancer cells via targeting CD44.ConclusionThe results contribute in the prognosis of brain cancer patients towards early diagnosis, intervention and precise medication, and the proposal of cold atmospheric plasma as a novel onco-therapeutic against brain cancers.
Photosynthetic usable energy explains vertical patterns of biodiversity in zooxanthellate corals
Authors: López-Londoño Tomás , Gómez-Campo Kelly , Hernández-Pech Xavier , Enríquez Susana , Iglesias-Prieto Roberto 
Published: June 2022

Abstract
The biodiversity in coral reef ecosystems is distributed heterogeneously across spatial and temporal scales, being commonly influenced by biogeographic factors, habitat area and disturbance frequency. In contrast to terrestrial environments, a potential association between gradients of usable energy and biodiversity has received little empirical support for explaining such patterns. Here, we analyzed the productivity and biodiversity variation over depth gradients in symbiotic coral communities, whose members rely on the energy translocated by photosynthetic algal symbionts (zooxanthellae). A mechanistic model was used to simulate the depth-dependent variation in photosynthetic usable energy to corals and explore its relationship with gradients of species diversity, comparing reefs with contrasting water clarity and biodiversity patterns along global hotspots of marine biodiversity. The productivity-biodiversity model explained between 64% and 95% of the depth-related variation in coral species richness, indicating that much of the variation in coral biodiversity with depth is driven by changes in the fractional contribution of photosynthetically fixed energy by the zooxanthellae. These results suggest a fundamental role of solar energy availability and photosynthetic productivity on global-scale patterns of coral biodiversity and community structure across depths. Accordingly, the maintenance of water optical quality is fundamental to protect coral biodiversity and prevent reef degradation, in addition to climate change mitigation policies.
Evaluation of new antimicrobial products based on potassium monopersulfate for disinfection of poultry farms
Authors: Moraes Miguel Augusto , Oliveira-Silva Mariana , Goulart Rafael Silva , Gabarra Manoel Henrique Cintra , Miranda Carlos Eduardo Saraiva , Almeida Paulo Garcia , Pitondo-Silva André 
Published: June 2022

Abstract
Bird drinkers used on poultry farms are highly susceptible to the formation of bacterial biofilms which can cause several diseases for animals and humans. This study evaluated the antimicrobial activity of new potassium monopersulfate-based products against bacterial biofilms. Four monopersulfate-based products named as P.A, P.B, P.C, and P.D. developed by the Hidrodomi do Brasil industry were evaluated. The products were tested against avian pathogenic Escherichia coli, Salmonella enteritidis, and Listeria monocytogenes mature biofilms. To verify the antimicrobial activity of the products, tests were initially performed against planktonic bacterial cells to determine their minimum inhibitory and bactericidal concentration. In the next step, the antimicrobial activity of the products was further evaluated in biofilms grown on acrylic specimens by counting colony-forming units (CFU) and also by employing both scanning electron microscopy (SEM) and confocal fluorescence microscopy. All products showed effectiveness against the bacterial species tested. It has been observed that P.A with concentrations lower than 0.13% presented the most effective action against planktonic cells and biofilms showing efficiency in inhibiting bacterial growth. P.A was able to completely eliminate both S. enteritidis and L. monocytogenes mature biofilms. The evaluated products, notably P. A., showed potential to be used as sanitizers and disinfectants on poultry farms.