bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety risk sources of autonomous mobile machines
Authors: Malm Timo , Tiusanen Risto , Heikkilä Eetu , Sarsama Janne 
Published: December 2022

Abstract
Autonomous mobile machines are applied increasingly often in outdoor applications, but only in places, where logistic problems and safety issues are resolvable. Solving safety issues require good hazard identification and risk estimation. The aim of this study is to show which new risks emerge in autonomous mobile machines compared to manual machines. There are new risks, which are valid only in autonomous mobile machines (e.g., related to sensors for situational awareness), and risks, which exist in both machine types, but human presence can make the difference in resolving the situation. Humans can take sophisticated protective measures during an unexpected hazardous situation. Another aim of this work is to give information to designers and assessors to consider risks of their own design. Autonomous mobile machines are complex, and their performance is not always easily predictable. A checklist of risks would help an assessor, but currently, it may be difficult to find a checklist for a specific autonomous mobile machine. The main result of this study is a checklist of new risk sources or hazards of autonomous mobile machines.
Quantitative Fluorescence Analysis Reveals Dendrite-Specific Thalamocortical Plasticity in L5 Pyramidal Neurons during Learning
Authors: Ray Ajit , Christian Joseph A. , Mosso Matthew B. , Park Eunsol , Wegner Waja , Willig Katrin I. , Barth Alison L. 
Published: December 2022

High-throughput anatomic data can stimulate and constrain new hypotheses about how neural circuits change in response to experience. Here, we use fluorescence-based reagents for presynaptic and postsynaptic labeling to monitor changes in thalamocortical synapses onto different compartments of layer 5 (L5) pyramidal (Pyr) neurons in somatosensory (barrel) cortex from mixed-sex mice during whisker-dependent learning (Audette et al., 2019). Using axonal fills and molecular-genetic tags for synapse identification in fixed tissue from Rbp4-Cre transgenic mice, we found that thalamocortical synapses from the higher-order posterior medial thalamic nucleus showed rapid morphologic changes in both presynaptic and postsynaptic structures at the earliest stages of sensory association training. Detected increases in thalamocortical synaptic size were compartment specific, occurring selectively in the proximal dendrites onto L5 Pyr and not at inputs onto their apical tufts in L1. Both axonal and dendritic changes were transient, normalizing back to baseline as animals became expert in the task. Anatomical measurements were corroborated by electrophysiological recordings at different stages of training. Thus, fluorescence-based analysis of input- and target-specific synapses can reveal compartment-specific changes in synapse properties during learning.SIGNIFICANCE STATEMENTSynaptic changes underlie the cellular basis of learning, experience, and neurologic diseases. Neuroanatomical methods to assess synaptic plasticity can provide critical spatial information necessary for building models of neuronal computations during learning and experience but are technically and fiscally intensive. Here, we describe a confocal fluorescence microscopy–based analytical method to assess input, cell type, and dendritic location-specific synaptic plasticity in a sensory learning assay. Our method not only confirms prior electrophysiological measurements but allows us to predict functional strength of synapses in a pathway-specific manner. Our findings also indicate that changes in primary sensory cortices are transient, occurring during early learning. Fluorescence-based synapse identification can be an efficient and easily adopted approach to study synaptic changes in a variety of experimental paradigms.
ST3GAL5‐catalyzed gangliosides inhibit TGF‐β‐induced epithelial‐mesenchymal transition via TβRI degradation
Authors: Zhang Jing , van der Zon Gerard , Ma Jin , Mei Hailiang , Cabukusta Birol , Agaser Cedrick C , Madunić Katarina , Wuhrer Manfred , Zhang Tao , ten Dijke Peter 
Published: December 2022

AbstractEpithelial‐mesenchymal transition (EMT) is pivotal in the initiation and development of cancer cell metastasis. We observed that the abundance of glycosphingolipids (GSLs), especially ganglioside subtypes, decreased significantly during TGF‐β‐induced EMT in NMuMG mouse mammary epithelial cells and A549 human lung adenocarcinoma cells. Transcriptional profiling showed that TGF‐β/SMAD response genes and EMT signatures were strongly enriched in NMuMG cells, along with depletion of UDP‐glucose ceramide glucosyltransferase (UGCG), the enzyme that catalyzes the initial step in GSL biosynthesis. Consistent with this finding, genetic or pharmacological inhibition of UGCG promoted TGF‐β signaling and TGF‐β‐induced EMT. UGCG inhibition promoted A549 cell migration, extravasation in the zebrafish xenograft model, and metastasis in mice. Mechanistically, GSLs inhibited TGF‐β signaling by promoting lipid raft localization of the TGF‐β type I receptor (TβRI) and by increasing TβRI ubiquitination and degradation. Importantly, we identified ST3GAL5‐synthesized a‐series gangliosides as the main GSL subtype involved in inhibition of TGF‐β signaling and TGF‐β‐induced EMT in A549 cells. Notably, ST3GAL5 is weakly expressed in lung cancer tissues compared to adjacent nonmalignant tissues, and its expression correlates with good prognosis.
p38 MAPK‐dependent phosphorylation of TFEB promotes monocyte‐to‐macrophage differentiation
Authors: Martina José A , Jeong Eutteum , Puertollano Rosa 
Published: December 2022

AbstractThe transcription factor EB (TFEB) regulates energy homeostasis and cellular response to a wide variety of stress conditions, including nutrient deprivation, oxidative stress, organelle damage, and pathogens. Here we identify S401 as a novel phosphorylation site within the TFEB proline‐rich domain. Phosphorylation of S401 increases significantly in response to oxidative stress, UVC light, growth factors, and LPS, whereas this increase is prevented by p38 MAPK inhibition or depletion, revealing a new role for p38 MAPK in TFEB regulation. Mutation of S401 in THP1 cells demonstrates that the p38 MAPK/TFEB pathway plays a particularly relevant role during monocyte differentiation into macrophages. TFEB‐S401A monocytes fail to upregulate the expression of multiple immune genes in response to PMA‐induced differentiation, including critical cytokines, chemokines, and growth factors. Polarization of M0 macrophages into M1 inflammatory macrophages is also aberrant in TFEB‐S401A cells. These results indicate that TFEB‐S401 phosphorylation links differentiation signals to the transcriptional control of monocyte differentiation.
Growth hormone deficiency in adult survivors of childhood brain tumors treated with radiation
Authors: Marie Baunsgaard Mette , Sophie Lind Helligsoe Anne , Tram Henriksen Louise , Stamm Mikkelsen Torben , Callesen Michael , Weber Britta , Hasle Henrik , Birkebæk Niels 
Published: December 2022

Objective
Growth hormone deficiency (GHD) is the most common endocrine late effect in irradiated survivors of childhood brain tumors. This study aimed to determine the prevalence of GHD in adults treated with proton or photon irradiation for a brain tumor in childhood and to detect undiagnosed GHD.

Design
This study is a cross-sectional study.

Methods
We investigated GHD in 5-year survivors from two health regions in Denmark treated for childhood brain tumors with cranial or craniospinal irradiation in the period 1997–2015. Medical charts were reviewed for endocrinological and other health data. Survivors without a growth hormone (GH) test at final height were invited to a GH stimulation test.

Results
Totally 41 (22 females) survivors with a median age of 21.7 years (range: 15.1–33.8 years) at follow-up and 14.8 years (range: 5.1–23.4 years) since diagnosis were included; 11 were treated with proton and 30 with photon irradiation; 18 of 21 survivors were previously found to have GHD; 16 of 20 survivors with no GH test at final height were tested, 8 (50 %) had GHD. In total, 26 of 41 patients (63%) had GHD. Insulin-like growth factor-1 (IGF-1) is associated poorly with the insulin tolerance test (ITT).

Conclusion
This study identified a high prevalence of undiagnosed GHD in survivors with no GH test at final height. The results stress the importance of screening for GHD at final height in survivors of childhood brain tumors with prior exposure to cranial irradiation, irrespective of radiation modality and IGF-1.

Significance statement
This cross-sectional study reports a prevalence of 63% of GHD in irradiated childhood brain tumor survivors. Furthermore, the study identified a considerable number of long-term survivors without a GH test at final height, of whom, 50% subsequently were shown to have undiagnosed GHD. Additionally, this study confirmed that a normal serum IGF-1 measurement cannot exclude the diagnosis of GHD in irradiated survivors. This illustrates the need for improvements in the diagnostic approach to GHD after reaching final height in childhood brain tumor survivors at risk of GHD. In summary, our study stresses the need for GHD testing in all adult survivors treated with cranial irradiation for a brain tumor in childhood irrespective of radiation modality.
Characterization of high-strength concrete under monotonic and fatigue mode II loading with actively controlled level of lateral compression
Authors: Becks Henrik , Aguilar Mario , Chudoba Rostislav , Classen Martin 
Published: December 2022

AbstractThe fatigue behavior of plain concrete has been studied for decades, usually under compressive or tensile loading. Shear loading (mode II) has been almost completely neglected in the past. In contrast to cylindrical compression tests, this type of loading offers the advantage of precise load determination and a small, well-defined fracture surface. This paper presents a comprehensive experimental campaign of 66 shear tests, which was conducted to systematically investigate the monotonic, cyclic, and fatigue response of high-strength concrete under mode II loading. Since the material behavior under shear stress is strongly dependent on the concurrent lateral compressive stress, a new test setup was developed which allows simultaneous control of compressive and shear loading. One potential utilization for these shear fatigue tests is the validation of a promising hypothesis that suggests that the development of fatigue damage in concrete at subcritical load levels is governed by a cumulative measure of shear sliding. The qualitative influence of the lateral compressive loading on the displacement and damage development, fracture behavior, and fatigue life is analyzed and discussed. The test results indicate that there is no influence of the lateral compressive load level on the shear fatigue life, as long as the increase in shear strength is considered. Furthermore, concrete under mode II loading seems to have a longer fatigue life than concrete in standard cylindrical specimens under compressive loading.
Studying the Inter-Peak Latencies of Auditory Brainstem Response in Menopause Women
Authors: Emami Seyede Faranak 
Published: December 2022

Background: Changes in auditory function have been noted in post-menopausal women attributed in part to the lower levels of ovarian hormones. Decreased levels of ovarian hormones may alter auditory neurotransmission time, as evaluated by Auditory Brainstem Responses (ABR). Thus, objective of this study was comparison of mean inter-peak ABR latencies in post-menopausal women compared to non-menopausal women.
Methods: In this cross-sectional study, research sample consisted of 60 women as case group in the age range of 45-55 years, who were post-menopausal and had normal hearing. The control group with similar characteristics were non-menopausal. Two groups were estimated by ABR and then the means of the variables that had a normal distribution were compared with each other by independent t-test.
Results: All differences between two groups were not significant, as follows; Mean I-III inter-peak ABR latencies (p-value=0.714), mean III-V inter-peak ABR latencies (p-value=0.691) and mean I-V inter-peak ABR latencies (p-value=0.483).
Conclusion: Menopause does not cause abnormal results in auditory brainstem responses.
Evaluation of the Prevalence of Irritable Bowel Syndrome in Patients with Rheumatoid Arthritis
Authors: Daraei Morteza , Afshari Ali , Alesaeidi Samira , Naserghandi Alvand , Allameh Seyed Farshad 
Published: December 2022

Background: Changes in auditory function have been noted in post-menopausal women attributed in part to the lower levels of ovarian hormones. Decreased levels of ovarian hormones may alter auditory neurotransmission time, as evaluated by Auditory Brainstem Responses (ABR). Thus, the objective of this study was to compare the mean inter-peak ABR latencies and post-menopausal women compared to non-menopausal women.
Methods: In this cross-sectional study, research sample consisted of 60 women as case group in the age range of 45-55 years, who were post-menopausal and had normal hearing. The control group with similar characteristics were non-menopausal. Two groups were estimated by ABR and then the means of the variables that had a normal distribution were compared with each other by independent t-test.
Results: All differences between two groups were not significant, as follows; Mean I-III inter-peak ABR latencies (p-value=0.714), mean III-V inter-peak ABR latencies (p-value=0.691) and mean I-V inter-peak ABR latencies (p-value=0.483).
Conclusion: Menopause does not cause abnormal results in auditory brainstem responses.
Association of Surgeon Age and Experience with Pilonidal Sinus Operation Technique
Authors: GÜNDOĞDU Ramazan , TOPRAK Uğur 
Published: December 2022

Objective: Most surgeons continue to use classical methods of surgical treatment despite the introduction of minimally invasive approaches. This study aimed to evaluate the association of surgeon age and experience on Pilonidal sinus (PS) treatment method.
Methodology: Clinical data of patients who presented with PS and underwent surgical treatment between January 2011 and November 2021 in our hospital were retrospectively analyzed. Operation method was classified as classical or minimally invasive. Surgeons were divided into three age groups: 28–35 years, 36–45 years, and ≥46 years. Drain use, anesthesia type, and operation method were compared between age groups. Correlation of surgeon age and these variables was also evaluated.
Results: In total, 253 patients were included for analysis (203 men and 50 women). Median age was 25 years (range, 16–64). The number of patients in the classical and minimally invasive groups was 155 and 98 patients, respectively. Use of minimally invasive surgery increased (r = 0.153) and drain use decreased (r = −0.232) as surgeon age and experience increased. Spinal anesthesia was the most frequently used anesthesia method; only 7.1% of patients underwent surgery using local anesthesia. There was no significant correlation between surgeon age and anesthesia type.
Conclusion: Classical surgical treatment of PS remains more common than minimally invasive approaches in our hospital. Use of minimally invasive approaches increased in conjunction with surgeon age and experience.
Observation on the effect of Intraoperative risk Management merged with comfort Nursing in Gynecological Laparoscopic surgery
Authors: Li Meixuan , Yao Xuan , Li Chao 
Published: December 2022

Abstract
Background
This research was devoted to estimating the outcome of intraoperative risk management merged with comfort nursing in gynecological laparoscopic surgery.
Methods
128 cases of laparoscopic surgery subjects (from February 2021 to February 2022) were opted form our hospital and divided into comfort care group and risk management group (n = 64/group). Comfort nursing intervention was taken in comfort care group, and risk management intervention was given in risk management group during operation. The coagulation indexes, immune inflammation indexes, the incidence of lower limb deep vein thrombosis (DVT) and the nursing satisfaction were compared.
Results
After intervention, versus those in the comfort care group, in the risk management group, APTT, PT and TT were longer, the counts of monocytes and neutrophils and the incidence of DVT were notablely restrained (P < 0.05). The nursing satisfaction was notablely higher (P < 0.05).
Conclusion
Intraoperative risk management merged with comfort nursing intervention can improve the level of coagulation indicators and immune inflammation indicators after gynecological laparoscopic surgery, reduce the incidence of DVT, and improve subject nursing satisfaction.
A hidden burden of disease in a specific group: Evaluation of COVID-19 seroconversion rates in pediatric patients with leukemia
Authors: Çelebi Miray Yılmaz , Şahinkaya Şahika , Cem Ela , Kara Aybüke Akaslan , Özdağ Eda , Ayhan Fahri Yüce , Karakaya Nurgül , Al Işık Odaman , Gözmen Salih , Karapınar Tuba Hilkay , Oymak Yeşim , Bayram Nuri , Devrim İlker 
Published: December 2022

Abstract
Background
SARS-CoV-2, a respiratory viral disease, is thought to have a more severe course in patients with malignancy and low immune systems.
Methods
This prospective single-center study was conducted at the University of Health Sciences Dr. Behçet Uz Children's Hospital from September 22 and December 31, 2021. Asymptomatic COVID-19 transmission rates were assessed using SARS-CoV-2 serology in patients with leukemia who had no history of COVID-19 infection.
Results
Among the 54 patients, 19 (35.2%) were females and 35 (64.8%) were males. The median age was 5.5 years (min 6 months, max 17 years). Forty-nine (90.5%) of the leukemia patients had acute lymphoblastic leukemia (ALL), while 5 (9.5%) had acute myeloid leukemia (AML). Five of the 54 patients had a history of COVID-19 or contact with a positive person. SARS-CoV-2 IgG positivity was detected in 18 (36.7%) of 49 patients with no history of COVID-19 infection.
Discussion
Leukemia patients have a high seroconversion for SARS-CoV-2 without showing any symptoms supporting the asymptomatic course of COVID-19 infection in this risk group.
Mental health challenges, treatment experiences, and care needs of post-secondary students: a cross-sectional mixed-methods study
Authors: Moghimi Elnaz , Stephenson Callum , Gutierrez Gilmar , Jagayat Jasleen , Layzell Gina , Patel Charmy , McCart Amber , Gibney Cynthia , Langstaff Caryn , Ayonrinde Oyedeji , Khalid-Khan Sarosh , Milev Roumen , Snelgrove-Clarke Erna , Soares Claudio , Omrani Mohsen , Alavi Nazanin 
Published: December 2022

Abstract
Background: Post-secondary students are at a greater risk of developing mental health problems than the general population. However, they present meagre rates of treatment-seeking behaviours. This elevated prevalence of mental health problems, particularly after the COVID-19 pandemic, can lead to distress, poor academic performance, and lower job prospects following the completion of their education. To address the needs of this population, it is important to understand students' perceptions of mental health and the barriers preventing or limiting their access to care.
Methods: A broad-scoping online survey was publicly distributed to post-secondary students, collecting demographic, sociocultural, economic, and educational information while assessing various components of mental health.
Results: In total, 448 students across post-secondary institutions in Ontario, Canada, responded to the survey. Over a third (n = 170; 38.6%) of respondents reported a formal mental health diagnosis. Depression and generalized anxiety disorder were the most commonly reported diagnoses. Most respondents felt that post-secondary students have poor mental health (n = 253; 60.5%) and inadequate coping strategies (n = 261; 62.4%). The most frequently reported barriers to care were financial (n = 214; 50.5%), long wait times (n = 202; 47.6%), insufficient resources (n = 165; 38.9%), time constraints (n = 148; 34.9%), stigma (n = 133; 31.4%), cultural barriers (n = 108; 25.5%), and past negative experiences with mental health care (n = 86; 20.3%). Most students felt their post-secondary institution needed to increase mental health resources (n = 306; 73.2%) and awareness (n = 231; 56.5%). Most students who had or were receiving care viewed in-person therapy as more helpful than online care. However, there was uncertainty about the helpfulness and accessibility of different forms of treatment, including online interventions.
Conclusions: Lack of resources, barriers to care, and uncertainty surrounding interventions contribute to the low treatment-seeking behaviours observed in post-secondary students. The survey findings indicate that multiple upstream approaches, including formal mental health education, may address the varying needs of this critical population. Online mental health interventions may be a promising solution to accessibility issues.
The effect of education based on the sexual self-concept model on sexual self-efficacy and sexual self-esteem of women after childbirth
Authors: Musavi Mana , Rahimzadeh Mitra , EsmaelzadehSaeieh Sara 
Published: December 2022

Abstract
Background
pregnancy, childbirth and postpartum period cause significant physical and psychological changes in mothers, these changes may have a destructive effect on women’s sexual self-concept and may decrease mother’s sexual self-efficacy and sexual self-esteem. Therefore, this study was designed with the aim of determining the effect of education based on the sexual self-concept model on sexual self-efficacy and sexual self-esteem of women after childbirth.
Methods
This parallel interventional study was conducted on 115 women who passed six weeks of their delivery and were referred to east health centers of Alborz university of medical sciences. Participants were randomly divided into two groups of intervention and control. Data gathering tool included Buzwell and Rosenthal’s Sexual Self-Efficacy Questionnaire (SSEQ), Buzwell and Rosenthal’s sexual Self-Esteem Questionnaire (SSEQ) and demographic characteristics form. The intervention group went through six online training sessions (each session lasting 45 minutes) within one month. While the participants in the control group only received the routine postpartum care. The sexual self-efficacy, sexual self-esteem and demographic characteristics questionnaires were completed by all participants before, after and one month after the intervention.
Results
the findings of this study showed that, there was no significant difference in demographic characteristics, sexual self-efficacy score and sexual self-esteem score in two groups before the intervention. The result of the ANOVA with repeated measures showed that the score of sexual self-efficacy and sexual self-esteem increased significantly over time in the intervention group immediately after participating in the training sessions and one month later.
Conclusion
Proper implementation of education based on the sexual self-concept model improved sexual self-efficacy and sexual self-esteem of women in the postpartum period.