bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alterations of bone proteins in medication‐related osteonecrosis of the jaw
Authors: Schubert Andrea , Brockmeyer Phillipp , Kauffmann Philipp , Wiegel Jan , Lautenbacher Florian , Miosge Nicolai , Schminke Boris 
Published: February 2025

AbstractChanges in the protein expression pattern of osteoblastic lineage cells from the alveolar bone (OLAB) during medication‐related osteonecrosis of the jaw (MRONJ) have rarely been investigated. This lack of information is partly because of the limited availability of healthy samples and the lack of human alveolar bone cell lines for research. The aim of the present study was to investigate the bone proteins collagen 1, runt‐related transcription factor 2 (RUNX2), and tumor necrosis factor ligand superfamily member 11 (RANKL). Furthermore, we established a cell lineage of OLAB suitable for the analyses of protein expression. We used immunohistochemistry to determine protein expression patterns in vivo. OLAB were treated during culture with zoledronate or denosumab and analyzed by immunocytochemistry and western blotting. Collagen 1 was decreased in vivo in patients with MRONJ and in vitro by denosumab. Zoledronate reduced the level of RUNX2 in vitro. However, RANKL was not significantly affected by zoledronate or denosumab. The results of the present study will help us elucidate the cellular mechanisms of MRONJ. Although culture of OLAB with zoledronate and denosumab significantly altered the protein expression patterns, future research is needed to examine the effects of bone scaffolds, biofilms, and additional cell types mimicking in vivo conditions.
Microbial Cycling of Sulfur and Other Redox‐Sensitive Elements in Porewaters of San Clemente Basin, California, and Cocos Ridge, Costa Rica
Authors: Osorio‐Rodriguez Daniela , Pavia Frank J. , Utter Daniel R. , Quinan Matthew , Landry Kameko , Gomes Maya , Dalleska Nathan D. , Orphan Victoria J. , Berelson William M. , Adkins Jess F. 
Published: February 2025

ABSTRACTThe microbial recycling of organic matter in marine sediments depends upon electron acceptors that are utilized based on availability and energetic yield. Since sulfate is the most abundant oxidant once oxygen has been depleted, the sulfide produced after sulfate reduction becomes an important electron donor for autotrophic microbes. The ability of sulfide to be re‐oxidized through multiple metabolic pathways and intermediates with variable oxidation states prompts investigation into which species are preferentially utilized and what are the factors that determine the fate of reduced sulfur species. Quantifying these sulfur intermediates in porewaters is a critical first step towards achieving a more complete understanding of the oxidative sulfur cycle, yet this has been accomplished in very few studies, none of which include oligotrophic sedimentary environments in the open ocean. Here we present profiles of porewater sulfur intermediates from sediments underlying oligotrophic regions of the ocean, which encompass about 75% of the ocean's surface and are characterized by low nutrient levels and productivity. Aiming at addressing uncertainties about if and how sulfide produced by the degradation of scarce sedimentary organic matter plays a role in carbon fixation in the sediment, we determine depth profiles of redox‐sensitive metals and sulfate isotope compositions and integrate these datasets with 16S rRNA microbial community composition data and solid‐phase sulfur concentrations. We did not find significant correlations between sulfur species or trace metals and specific sulfur cycling taxa, which suggests that microorganisms in pelagic and oxic sediments may be generalists utilizing flexible metabolisms to oxidize organic matter through different electron acceptors.
A Novel Approach to Assess the Distance Between the Settlement Patterns of Two Populations
Authors: Mucciardi Massimo 
Published: February 2025

ABSTRACTUnderstanding settlement patterns and their spatial distribution is essential for various fields, including geography, demography, and sociology. This paper explores a novel approach to assess the distance between settlement patterns of two populations with a specific focus on territorial variation inequalities. Building upon the conceptual framework of the Lorenz curve, we introduce new indices and graphical representations that permit us to emphasize territorial differences in settlement between two populations. By emphasizing the distance between settlement patterns, this methodology captures variations and inequalities in territorial distribution. Rather than simply characterizing settlement patterns as concentrated or dispersed, this approach considers the extent to which populations are spatially separated or integrated. To test this new approach, three foreign reference communities were examined (Egyptians, Chinese, and Romanians) known in the literature for their markedly different settlement patterns in Italy. We identified three different patterns for these populations that highlight the importance of considering local variations and spatial interactions in the study of settlement patterns. The results obtained seem to agree with the theory of settlements of foreign populations in Italy, albeit with additional geographical information. Through this research, we aim to provide a new methodology for measuring the distance between the settlement patterns of two different populations, filling some gaps in traditional methods.
Lactic Acid Bacteria and Bacteriocins in Feed Preservation: Mechanisms and Antifungal Properties
Authors: Azevedo Pamela Oliveira de Souza de , Gierus Martin 
Published: February 2025

ABSTRACTEnsilage consists of the fermentation of sugars to lactic acid independent of the forage by epiphytic microorganisms, lactic acid bacteria (LAB), which vary in number and composition during fermentation. Silage is a means of preserving forage for extended periods of time when fresh forage is limited or seasonally unavailable, and its inadequate fermentation and poor feed management favour the proliferation of pathogens and contribute to silage spoilage. LAB is a group of microorganisms widely distributed in nature, with lactic acid as the main metabolite produced after sugar fermentation. Some LAB produce antimicrobial peptides with low toxicity and proteinaceous nature, known as bacteriocins, which have a broad spectrum of activity against Gram‐positive and Gram‐negative bacteria, protozoa, fungi, and viruses and can therefore be effective in silage preservation. Among those species, fungal growth leads to nutrient and dry matter losses, reduced palatability, reduced silage intake and consequently reduced animal performance. While laboratory studies provide compelling evidence of their antifungal properties, the practical application of LAB in silage systems must be evaluated with an understanding of the complexities of real‐world conditions. LAB inoculants primarily enhance silage quality by driving acidification and promoting a desirable fermentation profile. While bacteriocins can provide an additional layer of microbial control, their efficacy is best realised when combined with proper management practices. This integrated approach ensures that the conditions for optimal fermentation are maintained, allowing LAB to exert their beneficial effects effectively. However, the complexities of silage fermentation necessitate a broader perspective that includes environmental management and forage quality. LAB and bacteriocins are valuable tools, but their success depends on their integration into a comprehensive silage management strategy.
Should Asystasia gangetica No Longer Be Considered Just a Weed but Rather Also a Livestock Fodder?
Authors: Rahman Mohammad Mijanur , Sultana Maliha , Len Candyrine Su Chui 
Published: February 2025

ABSTRACTUnder oil palm, rubber, and coconut plantations, there are many plant species that are commonly considered weeds. Ruminants are often grazed on these palatable weeds as the primary source of feed on such integrated farms. Asystasia gangetica (L.) T. Anderson belongs to the family Acanthaceae, flowering plants predominantly found in tropical and subtropical regions. This plant is perennial and invasive and extensively grows on the ground surface of tree plantations. Redefining A. gangetica as a forage crop instead of a weed can support sustainable livestock feeding by utilising its nutritional value, adaptability, and cost‐saving benefits, contributing to climate‐smart animal production. It has been revealed that this plant has high nutritional value and biologically active compounds. In recent years, there has been growing interest in using A. gangetica as a low‐input management plant for animal feeding purposes. Due to its weedy characteristics and limited use as an animal feed, data on growth characteristics, nutritional value, and utilisation of the species in tropical regions is insufficient. In this paper, the distribution and adaptation of A. gangetica are determined, and the growth characteristics, nutritive value, and usefulness assessed. This species is relatively short and spreads rapidly forming a dense sward, which is important for the prevention of soil erosion thus having potential also as a cover crop.
Carbonylation of hair proteins: A robust biomarker of molecular and structural oxidative damage in hair fibres
Authors: Grosjacques Camille , Babiel Sabine , Hodes Jing , Bobier Anais , Cavagnino Andrea , Baraibar Martin 
Published: February 2025

AbstractObjectiveThe first objective of this study is to compare two molecular markers, cysteic acid and protein carbonylation, to track the level of chemical oxidation and photochemical (UV) oxidation of human hair. The second objective is to investigate how the protein carbonylation biomarker evolution is associated with physical characteristics of the fibre. The third objective is to understand the damage localization within the hair sub‐structure.Materials and MethodsFor the chemical oxidation study European natural hair 6/0 (medium brown) is treated with non‐pigmented commercial hair colour mixture and hair bleaching in increasing chemical oxidation conditions. For the photochemical study two European natural hair colours, 4/0 (dark brown) and 9/0 (extra light blond) are gradually photo irradiated. Molecular changes are investigated through cysteic acid measurement by NIR spectroscopy and protein carbonylation measurement; Biophysical changes by differential scanning calorimetry (DSC), and tensile strength measurements. Carbonyls are labelled with a specific fluorescent probe and measured in gel electrophoresis and in situ through image analysis and densitometric quantification after protein extraction respectively.ResultsThe two molecular markers, cysteic acid and protein carbonylation, increase similarly in both chemical and photochemical oxidation cases and show a good level of association across the oxidation levels. The fibre physical characteristics (DSC, Tensile Strength) decrease while the protein carbonylation and cysteic acid increase. The in situ visualization of the protein carbonylation shows a high impact on the hair cuticle and a gradual increase of photo‐oxidation through the cortex, phenomenon which is more prominent for the extra light blonde hair in the case of photochemical oxidation.ConclusionThe protein carbonylation biomarker is validated as another key molecular marker to monitor oxidative chemical changes in the hair chemical groups. It complements the cysteic acid, and appears more suitable in the case of photochemical oxidation, where it offers clear advantages over cysteic acid by being more sensitive and accurate, and by allowing in situ distinct damage visualization. Besides cysteic acid, amino acids such as proline, threonine, arginine, lysine, and peptide bonds are targets of oxidation. Under photochemical oxidation, the photoprotective effect of melanin is confirmed.
Cardiac Neuromodulation and Neurocardiology
Authors: Lemery Robert 
Published: February 2025

ABSTRACTNeurocardiology has mostly been a specialty of medicine led by anatomists and physiologists. The characterization of the cardiac autonomic nervous system has resulted in a new understanding and appreciation of neurocardiology, leading to potential novel neuromodulation therapies in clinical cardiology and cardiac electrophysiology. Sympathectomy or spinal cord stimulation for the treatment of angina pectoris, as well as cardiac sympathetic denervation for the treatment of long QT syndrome associated with malignant ventricular arrhythmias, have been available and performed for more than half a century. However, a new neuromodulation has emerged, based on contemporary research findings, assisted by state‐of‐the art imaging and ablation techniques. Patients with structural heart disease and malignant ventricular arrhythmias, as well as symptomatic ventricular ectopy, can potentially benefit from techniques to reduce autonomic tone, such as stellate ganglionic block, epidural anesthesia and cardiac sympathetic denervation. Renal sympathetic denervation not only has been shown to ameliorate the treatment of patients with hypertension, but may also reduce atrial and ventricular arrhythmias. Patients with heart failure may be improved clinically by potentiating parasympathetic tone. Cardiac mapping of ganglia and nerves can be performed to delineate regions of ablation that can suppress atrial fibrillation, and potentially treat symptomatic bradyarrhythmias and cardio‐inhibitory syncope.
Hyperuricemia Exacerbates Experimental Periodontitis via Uric Acid‐Induced Periodontal Inflammation and Oxidative Stress
Authors: Wu Zhicong , Zhao Li , Guo Yi , Lin Chuyin , Lu Peipei , He Qian , Zhou Yinghong , Wang Xinhong , Yu Ting 
Published: February 2025

ABSTRACTAimTo investigate the effects of hyperuricemia on periodontitis and the underlying mechanisms by establishing combined animal and cell models.MethodsA hyperuricemia mouse model was established by potassium oxonate injection, with sodium carboxymethylcellulose treatment serving as controls. Both models were treated with or without periodontitis induction (n = 10/group). RAW264.7 macrophages and THP‐1‐derived macrophages were stimulated with Porphyromonas gingivalis‐lipopolysaccharide in the presence of normal or excessive concentrations of uric acid. Allopurinol intervention was applied to both animal and cell models. Periodontal destruction was measured by micro‐computed tomography and histology. The immune response and oxidative stress in the periodontium and macrophages were assessed using various methods including immunohistochemistry, quantitative PCR, western blotting, flow cytometry and multiplex cytokine assays.ResultsPotassium oxonate successfully induced hyperuricemia without affecting serum glucose/lipid levels or xanthine oxidoreductase activity. In mice with periodontitis, hyperuricemia exacerbated alveolar bone loss and the presence of osteoclasts and M1 macrophages. Mechanistically, hyperuricemia promoted NLRP3 inflammasome activation, disrupted the inflammatory cytokine response and exacerbated oxidative stress both in the periodontium and in vitro. Allopurinol treatment reversed all relevant changes in both mice and macrophages.ConclusionHyperuricemia exacerbates periodontitis possibly via uric acid‐induced periodontal inflammation and oxidative stress.
Effects of Aging on the Severity of Liver Injury in Mice With Iron Overload
Authors: Park So‐Hyun , Song Soo‐Jin , Lee Jin‐A , Shin Jung‐A 
Published: February 2025

ABSTRACTAlthough iron is a vital component in the body, excessive iron leads to iron toxicity, which affects vital organs. In particular, the liver is considerably affected by iron toxicity because it stores the highest amount of iron in the body. Nonetheless, the relationship between iron overload and aging in the liver has not yet been clearly identified. This study aimed to observe the effects of aging on iron overload in the liver. Female C57BL/6J mice were randomly divided into vehicle control and iron overload groups (n = 7–22 per group). The iron overload group was injected with iron‐dextran (Fe‐dextran, ferric hydroxide dextran complex) (0.5 g/kg) for 4 weeks. After the experimental period, liver and blood samples were obtained from 2‐, 15‐, and 22‐month‐old mice. Liver weight, iron deposition, structural changes, cell death, extracellular matrix deposition, and fenestration of sinusoidal vessels were analyzed and compared between the groups. Additionally, biochemical analyses (aspartate aminotransferase, alanine aminotransferase, and serum total iron levels) were performed. The iron overload group exhibited significant differences compared with the control group with age. In the elderly iron overload model, iron deposition, inflammatory cell infiltration, and cell death were significantly increased (p < 0.0001). Moreover, deposition of the extracellular matrix and defenestration of sinusoidal fenestrae were observed among 22‐month‐old mice in the iron overload group. These results suggest that aging is a risk factor for iron‐induced liver injury. Therefore, caution should be exercised when performing iron‐related treatments in the elderly.
Effectiveness and Tolerability of an Emollient “Plus” Compared to Urea 10% in Patients With Mild‐to‐Moderate Atopic Dermatitis
Authors: Prakoeswa Cita Rosita Sigit , Huda Berliana Kurniawati Nur , Indrawati Ditya , Umborowati Menul Ayu , Anggraeni Sylvia , Damayanti  , Murtiastutik Dwi , Kerob Delphine 
Published: February 2025

ABSTRACTBackgroundAtopic dermatitis (AD) poses a challenge due to its chronic inflammatory nature. Recent research highlights microbiome dysbiosis as a key contributor. Emollients “plus” are modern moisturizers containing bacterial lysate, improving skin barrier function and reducing Staphylococcus aureus colonization, thus mitigating AD symptoms. Emollient “plus” containing Vitreoscilla filiformis biomass (Aqua Posae filiformis) is efficient in AD, as single adjunct for milder forms or adjunctive to systemic treatments in more severe forms. Standard recommended moisturizers for AD in Indonesia contain urea 10%.AimsThis trial compared an emollient “plus” (Group A) with urea 10% moisturizer (Group B) in the treatment of mild‐to‐moderate AD.Patients/MethodsSixty subjects with mild‐to‐moderate AD were randomized into Groups A and B (30 subjects/group). Test products were applied twice daily for 12 weeks. Clinical and instrumental endpoints assessed at Weeks 0, 4, 8, and 12 included Severity Scoring of AD (SCORAD), Pruritus Visual Analog Scale (PVAS), Eczema Area and Severity Index (EASI), Dermatology Life Quality Index (DLQI), Transepidermal Water Loss (TEWL), skin hydration, skin pH, as well as tolerance evaluation.ResultsSignificant differences in favor of the emollient “plus” versus urea 10% were observed on TEWL and skin pH values at Weeks 4, 8, and 12, on SCORAD and skin hydration values at Weeks 8 and 12. EASI, DLQI, and PVAS values differed significantly at Week 12 in favor of Group A. Both products were well tolerated.ConclusionsThis emollient “plus” has superior efficacy in improving AD symptoms and skin barrier function compared to urea 10% moisturizer.
Organizing and Strategizing in and for Extreme Contexts: Temporality, Emotions, and Embodiment
Authors: Hällgren Markus , Geiger Daniel , Rouleau Linda , Sutcliffe Kathleen M. , Vaara Eero 
Published: February 2025

AbstractThis special issue advances our understanding of organizing and strategizing in extreme contexts by focusing on temporality, emotions, and embodiment. Extreme contexts – marked by unpredictability, high stakes, and urgency – challenge organizational capacities and demand innovative responses. Drawing on the foundation of extreme context research, this introduction explores three perspectives: extreme as an event, a situational context, and a socially constructed practice. Together, these perspectives illuminate how organizations navigate, adapt to, and construct extremeness through temporal, emotional, and embodied processes. The contributions span diverse empirical settings and theoretical frameworks. By examining the contributions in the light of these dimensions, this introduction highlights the evolving and contested nature of extreme context research. The introduction concludes with a call for future studies to deepen engagement with materiality, relational dynamics, and methodological innovations, reinforcing the relevance of this field to broader management and organization studies.
The Impact of Usage Method and Coffee Variety on Consumer Preferences of Coffee Creamers
Authors: Rovai Dominic , Homwongpanich Kumpol , Watson Megan , Drake MaryAnne 
Published: February 2025

ABSTRACTConsumer evaluation of coffee creamers necessitates evaluation in a cup of coffee. Two phases of research were used to determine if method of serving creamer or coffee variety impacted the acceptance of four creamers. In Phase I, consumers evaluated creamers in medium roast coffee using one of three methods: (1) Fixed amount (n = 127), (2) free pour (n = 120), and (3) warm‐up (n = 122). In Phase II, the serving method was constant (fixed amount), but consumers (n = 134) evaluated creamers in two coffees (light and dark roast). Data were analyzed using univariate and multivariate statistics. No significant interactions (p > 0.05) were observed for overall liking (key metric) for either creamer × method (Phase I) or creamer × coffee (Phase II). Thus, the choice of serving method will not impact creamer preference. Further, the choice of coffee roast will not impact creamer preference, but creamers served in light roast coffee had higher liking scores than creamers served in dark roast coffee.
Analysis of sleep medication‐induced parasomnias using the Japanese Adverse Drug Event Report database
Authors: Kobayashi Shotaro , Shinozaki Koji , Nagano Hiroyuki , Miyamori Ayaka , Muramatsu Taichi , Kushiyama Akifumi 
Published: February 2025

SummaryThe association between sleep medication and parasomnia remains unclear. However, some sleep medications may also cause parasomnia. Therefore, we examined the association between sleep medication use and parasomnia. We performed an analysis using cases registered in the Japanese Adverse Drug Event Report Database from April 2004 to March 2022. We calculated the unadjusted reporting odds ratio (ROR) and adjusted it to consider confounding factors. The ROR for each sleep medication was significant: benzodiazepines (ROR 3.55, 95% confidence interval [CI] 2.39–5.27), non‐benzodiazepines (ROR 14.21, 95% CI 10.41–19.39), melatonin receptor agonists (ROR 6.79, 95% CI 2.79–16.51), orexin receptor antagonists (ROR 18. 40, 95% CI 10.66–31.75). The adjusted RORs for non‐benzodiazepines (9.75, 95% CI 6.71–14.2) and orexin receptor antagonists (8.65, 95% CI 4.56–16.4) were significant for sleep medications. These findings indicate that non‐benzodiazepines and orexin receptor antagonists may cause parasomnias. Significant signals were detected, even when confounding factors were considered.