bluuebunny/crossref_metadata_2025_split huggingface dataset:
The reciprocal relationship between episodic memory and future thinking: How the outcome of predictions is subsequently remembered
Authors: Patel Sneh P. , McCurdy Matthew P. , Frankenstein Andrea N. , Sklenar Allison M. , Urban Levy Pauline , Szpunar Karl K. , Leshikar Eric D. 
Published: August 2022

AbstractEvidence suggests that memory is involved in making simulations and predictions about the future (i.e., future thinking), but less work has examined how the outcome of those predictions (whether events play out as predicted or expected) subsequently affects episodic memory. In this investigation, we examine whether memory is better for outcomes that are consistent with predictions, or whether memory is enhanced for outcomes that are inconsistent with predictions, after the predicted event occurs. In this experiment, participants learned a core trait associated with social targets (e.g., high in extroversion), before making predictions about behaviors targets would perform. Participants then were shown behaviors the social targets actually performed (i.e., prediction outcome), which was either consistent or inconsistent with predictions. After that, participants completed a memory test (recognition; recall) for the prediction outcomes. For recognition, the results revealed better memory for outcomes that were consistent with traits associated with targets (i.e., trait‐consistent outcomes), compared to outcomes that were inconsistent (i.e., trait‐inconsistent outcomes). Finding a memory advantage for trait‐consistent outcomes suggests that outcomes that are in line with the contents of memory (e.g., what one knows; schemas) are more readily remembered than those that are inconsistent with memory, which may reflect an adaptive memory process. For recall, memory did not differ between trait‐consistent and trait‐inconsistent outcomes. Altogether, the results of this experiment advance understanding of the reciprocal relationship between episodic memory and future thinking and show that outcome of predictions has an influence on subsequent episodic memory, at least as measured by recognition.
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis
Authors: Rawat Jyoti , Bhambri Aksheev , Pandey Ujjiti , Banerjee Sanchita , Pillai Beena , Gadgil Mugdha 
Published: August 2022

AbstractAmino acid compositions of cell culture media are empirically designed to enhance cell growth and productivity and vary both across media formulations and over the course of culture due to imbalance in supply and consumption. The interconnected nature of the amino acid transporters and metabolism suggests that changes in amino acid composition can affect cell physiology. In this study, we explore the effect of a step change in amino acid composition from a DMEM: F12‐based medium to a formulation varying in relative abundances of all amino acids, evaluated at two amino acid concentrations (lean LAA vs. rich HAA). Cell growth was inhibited in LAA but not HAA. In addition to the expected effects on expression of the cell cycle, amino acid response and mTOR pathway genes in LAA, we observed an unanticipated effect on zinc uptake and efflux genes. This was accompanied by a lower tolerance to zinc supplementation in LAA but not in the other formulations. Histidine was sufficient but not necessary to prevent such zinc toxicity. Additionally, an unanticipated downregulation of genes in the cholesterol synthesis pathway was observed in HAA, accompanied by an increase in cellular cholesterol content, which may depend on the relative abundances of glutamine and other amino acids. This study shows that changes in the amino acid composition without any evident effect on growth may have profound effects on metabolism. Such analyses can help rationalize the designing of medium and feed formulations for bioprocess applications beyond replenishment of consumed components.
Willingness to pay for packaging cancer screening of Chinese rural residents
Authors: Zhang Qianqian , Ren Deyu , Chang Xuan , Sun Chen , Liu Ruyue , Wang Jialin , Zhang Nan 
Published: August 2022

AbstractBackgroundThis study examined the acceptance and willingness to pay (WTP) of rural residents toward packaging cancer screening (PCS) to provide a reference basis for promoting the screening sustainable development.MethodsA face‐to‐face questionnaire survey was conducted among rural residents aged 40–69. The combination of double‐bounded dichotomous choices and open‐ended questions in the Contingent Valuation Method was used to guide participants' WTP. Logistic regression was used to explore the influencing factors of participants' screening acceptance, and Tobit model was used to analyze the associated factors of WTP.ResultsOf the 959 respondents, 89.36% were willing to accept PCS, but 10.64% stated unwillingness for the dominant reason that they did not attend clinics until symptom onset. Willingness to accept screening was significantly associated with region (Dongchangfu, OR = 0.251, 95%CI: 0.113~0.557; Linqu, OR = 0.150, 95%CI: 0.069~0.325), age with 60–69 (OR = 0.321, 95%CI: 0.126~0.816), annual income with 10,000–30,000 (OR = 1.632, 95%CI: 1.003~2.656) and having cancer‐screening experience (OR = 0.581, 95%CI: 0.371~0.909). And 57.66% of participants were willing to pay part of the screening cost among those willing to accept PCS. The residents' average WTP was ¥622, accounting for 20.73% of the total cost (¥3000). Willingness to pay for PCS was positively correlated with male gender, self‐employed occupation, residence in Feicheng (than Linqu), higher income, and having cancer‐screening experience.ConclusionsMost rural residents were willing to accept free PCS, more than half of them were willing to pay part of the ¥3000 total cost, but their WTP‐values were low. It is necessary to carry out PCS publicity activities to improve public awareness and participation in precancerous screening. Additionally, for expanding the coverage and sustainability of screening, the appropriate proportion of rural residents to pay for screening costs should be controlled at about 20%, and governments, insurance and other sources are encouraged to actively participate to cover the remaining costs.
An in Vitro Cytomimetic of In‐Cell RNA Folding
Authors: Yoo Hyejin , Davis Caitlin M. 
Published: August 2022

AbstractTo discover the cytomimetic that accounts for cytoplasmic crowding and sticking on RNA stability, we conducted a two‐dimensional scan of mixtures of artificial crowding and sticking agents, PEG10k and M‐PERTM. As our model RNA, we investigate the fourU RNA thermometer motif of Salmonella, a hairpin‐structured RNA that regulates translation by unfolding and exposing its ribosome binding site (RBS) in response to temperature perturbations. We found that the addition of artificial crowding and sticking agents leads to a stabilization and destabilization of RNA folding, respectively, through the excluded volume effect and surface interactions. FRET‐labels were added to the fourU RNA and Fast Relaxation Imaging (FReI), fluorescence microscopy coupled to temperature‐jump spectroscopy, probed differences between folding stability of RNA inside single living cells and in vitro. Our results suggest that the cytoplasmic environment affecting RNA folding is comparable to a combination of 20 % v/v M‐PERTM and 150 g/L PEG10k.
Bacterial Biofilm Probed by Scanning Electrochemical Microscopy: A Review
Authors: Zhou Yan , Zhang Junjie , Zhao Yuxiang , Liu Yulin , Li Fei 
Published: August 2022

AbstractBacterial biofilm, a highly structured community with developmental processes from cell adherence to highly dense biofilms, is of critical concern in many industries. Investigations of parameters mediating biofilm formation, interactions between bacterial species, and parameters governing biofilm stability are quite important for understanding mechanism of biofilm formation. Scanning electrochemical microscopy (SECM), a local electrochemical technique using a microelectrode as its probe, can monitor microbe interactions in situ via detection of small molecules or biochemical cues with spatial and temporal resolution. In this review, we summarize the recent advances in biofilm research using SECM. First, we introduce the applications and potential biochemical cues of biofilms. Then, we classify the applications based on the bacterial species of interest, focusing on SECM to study key biochemical cues and highlighting underlying metabolic processes involved in biofilm formation. Finally, we discuss the challenges and limitations given the complexity and diversity of biofilms for future SECM studies.
Terpyridine Diphosphine Ruthenium Complexes as Efficient Photocatalysts for the Transfer Hydrogenation of Carbonyl Compounds
Authors: Ballico Maurizio , Alessi Dario , Jandl Christian , Lovison Denise , Baratta Walter 
Published: August 2022

AbstractThe cationic achiral and chiral terpyridine diphosphine ruthenium complexes [RuCl(PP)(tpy)]Cl (PP=dppp (1), (R,R)‐Skewphos (2) and (S,S)‐Skewphos (3)) are easily obtained in 85–88 % yield through a one‐pot synthesis from [RuCl2(PPh3)3], the diphosphine and 2,2′:6′,2′′‐terpyridine (tpy) in 1‐butanol. Treatment of 1–3 with NaPF6 in methanol at RT affords quantitatively the corresponding derivatives [RuCl(PP)(tpy)]PF6 (PP=dppp (1 a), (R,R)‐Skewphos (2 a) and (S,S)‐Skewphos (3 a)). Reaction of [RuCl2(PPh3)3] with (S,R)‐Josiphos or (R)‐BINAP in toluene, followed by treatment with tpy in 1‐butanol and finally with NaPF6 in MeOH gives [RuCl(PP)(tpy)]PF6 (PP=(S,R)‐Josiphos (4 a), (R)‐BINAP (5 a)) isolated in 78 % and 86 % yield, respectively. The chiral derivatives have been isolated as single stereoisomers and 3 a, 4 a have been characterized by single crystal X‐ray diffraction studies. The tpy complexes with NaOiPr display high photocatalytic activity in the transfer hydrogenation (TH) of carbonyl compounds using 2‐propanol as the only hydrogen donor and visible light at 30 °C, at remarkably high S/C (up to 5000) and TOF values up to 264 h−1. The chiral enantiomers 2, 2 a and 3, 3 a induce the asymmetric photocatalytic TH of acetophenone, affording (S)‐ and (R)‐1‐phenylethanol with 51 and 52 % ee, respectively, in a MeOH/2‐propanol mixture.
Design of a Photocatalytic [2+2] Cycloaddition Reaction Using Redox‐Tag Strategy
Authors: Hashimoto Yasuhiro , Horiguchi Genki , Kamiya Hidehiro , Okada Yohei 
Published: August 2022

AbstractThe design of photocatalytic processes is important for a sustainable society. Key to these photocatalytic reactions is electron transfer. This article is focused on titanium dioxide photocatalyzed organic synthesis and the design of a new [2+2] cycloaddition reaction based on the electron transfer process. Electron transfer ‐ not only between the substrate and the photocatalyst but also inter‐ and intramolecularly – is crucial for the reaction design. Radical cations were generated by the photocatalyst and trapped by alkenes. The resultant cyclobutyl radical cations were immediately reduced by the aryl rings via intramolecular electron transfer to obtain cyclobutane rings. The outcome of the reaction was controlled by substitution of the aryl ring and the linker connecting the aryl ring to the enol ether. The carefully designed substrates were highly effective for photocatalytic cycloaddition.
Engineering Multipedal DNA Walker on Paper for Sensitive Electrochemical Detection of Plant MicroRNA
Authors: Liu Xiaojuan , Xiang Junzhu , Cheng Hao , Wang Yuying , Li Feng 
Published: August 2022

Comprehensive SummaryMicroRNAs (miRNAs) are vital regulators in both plants and animals. Therefore, it is highly desirable to develop portable and user‐friendly biosensors for convenient and sensitive detection of miRNAs. Herein, a novel paper‐based electrochemical biosensor was intelligently engineered for the detection of plant miRNA based on a smart DNA walking machine and λ‐exonuclease (λ‐Exo)‐assisted target recycling amplification. Using TaMIR5086 as a target plant miRNA, the presence of TaMIR5086 could initiate the target recycling process and activate the DNA walker to move along the track on the paper. Then, numerous electroactive molecules‐labeled single‐strand DNA (ssDNA) would be released and adsorbed onto the surface of screen‐printed electrode, generating a remarkably increased electrochemical signal. Benefitting from the dual amplification, the developed biosensor exhibits excellent analytical performance toward TaMIR5086 with a detection limit down to 0.37 pmol/L. Furthermore, the paper‐based biosensor could be applied for the analysis of target miRNA in complex biological samples, which found great potential in the fields of miRNA analysis and plant biology research.
Many‐objective evolutionary algorithm assisted by a novel angle‐based fitness strategy
Authors: Zhu Zhuanghua 
Published: August 2022

SummaryMeasuring the convergence of solutions is greatly significant for many‐objective optimization problems. Although various methods have been proposed regarding this issue, they still suffer from the performance consistency on different problems. For this issue, this article proposes a novel angle‐based fitness strategy. To be specific, the convergence of solutions is defined with adaptive key solutions, which are determined by the angles between solutions. Based on the novel angle‐based fitness strategy, this article designs a many‐objective evolutionary algorithm assisted by a novel angle‐based fitness strategy. Experiments are conduced to verify the performance of the proposed method in comparison with other state‐of‐the‐art methods. The experimental analyzes illustrate the outstanding performance of the proposed method for many‐objective optimization problems.
Designing a Propellane‐based Nonlinear Optically Active System Absorbing in Three Different Wavelength Regions
Authors: Rajput Swati Singh , Alam Mehboob 
Published: August 2022

AbstractThe aim of this work is to demonstrate the possibility of using propellane in designing a molecule that can absorb in three different wavelength regions and their nonlinear optical (NLO) activity can be fine‐tuned by varying the three wings. We considered 22 tailor‐made propellane derivatives consisting of phenyl, naphthyl, and biphenyl wings for this purpose. Using the state‐of‐the‐art linear and quadratic response methods within TD‐DFT and RI‐CC2 theories and a suitable generalized few‐state model that quantifies the effect of orientation of different transition moments on NLO properties, we discussed how and why the linear and nonlinear optical activity of propellane vary when the three wings are assembled successively to construct a full‐propellane.
Walking the talk, but above all, talking the walk: Looking green for market stakeholder engagement
Authors: Amores‐Salvadó Javier , Martin‐de Castro Gregorio , Albertini Elisabeth 
Published: August 2022

AbstractIn recent years, corporate environmental commitment is showing different postures going from greenwashing to undue modesty and from environmental excellence to environmental inaction. In this paper, we go a step beyond the most recent research work on the dichotomy greenwashing–brownwashing and develop a more comprehensive model that reflect more subtly four main corporate environmental strategic positions based on both firm's environmental performance and disclosure achievements. Jointly with their characterization, and adopting a question‐driven approach we add to the literature on environmental disclosure‐firm performance proposing an explorative research question that consider cluster membership on market firm performance under the general assumption that, at least theoretically, each of the four main corporate environmental strategic positions under analysis can result in market performance improvements. Our empirical results from a panel data of international industrial companies show very interesting and novel insights, highlighting the fact that being green is good, but above all, it is good to look green.
Write‐enhanced and radiation‐hardened SRAM for multi‐node upset tolerance in space‐radiation environments
Authors: Zhao Qiang , Dong Hanwen , Peng Chunyu , Lu Wenjuan , Lin Zhiting , Chen Junning , Wu Xiulong 
Published: August 2022

SummaryAs transistor feature size is scaling down, the probability of charge sharing in a space‐radiation environment increases because of the reduced distance between adjacent transistors. The single‐event multiple‐node upset (SEMNU) caused by charge sharing is a major source of data errors in high‐density static random‐access memory (SRAM). In this paper, a radiation‐hardened SRAM using polarity hardening is proposed. Compared to other cells (RHPD‐12T, RSP14T, SEA14T, We‐Quatro, QUCCE12T, SARP12T, SIS10T, and 12T), the proposed RHC‐14T cell saves 8.47%, 91.34%, 162.71%, ‐20.63%, −20.50%, 113.18%, 63.27%, and 20.60% of the read‐delay time and 7.96%, 66.17%, 68.16%, 57.71%, 22.39%, 12.44%, 1,010.45%, and 13.43% of the write‐delay time, respectively. Moreover, this excellent performance entails only minimal power consumption. The proposed cell can work well in the radiation‐intensive space environment.
Molecular characterization of the immediate wound response of the solitary ascidian Polycarpa mytiligera
Authors: Hendin Noam , Gordon Tal , Shenkar Noa , Wurtzel Omri 
Published: August 2022

AbstractBackgroundInjury response is key to successful regeneration. Yet, transcriptome analyses of injury response were performed only on a handful of regenerative organisms. Here, we studied the injury response of the solitary ascidian Polycarpa mytiligera, an emerging model system, capable of regenerating any body part. We used the siphon as a model for studying transcriptional changes following injury, and identified genes that were activated in the initial 24 hours post amputation (hpa).ResultsHighly conserved genes, such as bone morphogenetic protein‐1 (BMP1), growth hormone secretagogue receptor (GHSR) and IL‐17, were upregulated by 12 hpa, yet their expression was sustained only in non‐regenerating tissue fragments. We optimized fluorescent in situ hybridization, and found that the majority of BMP1+ cells were localized to the rigid tunic that covers the animal. This highlights the importance of this tissue, particularly during injury response. BMP1 was overexpressed following injuries to other body regions, suggesting that it was a part of a common injury‐induced program.ConclusionOur study suggests that, initially, specific injury‐induced genes were upregulated in P. mytiligera organs, yet, later, a unique transcriptional profile was observed only in regenerating tissues. These findings highlight the importance of studying diverse regenerating and non‐regenerating organisms for complete understanding of regeneration.
What state of the world are we in? Targeted monitoring to detect transitions in vegetation restoration projects
Authors: Jones Christopher Stuart , Thomas Freya Mary , Michael Damian Richard , Fraser Hannah , Gould Elliot , Begley Jim , Wilson Jenny , Vesk Peter Anton , Rumpff Libby 
Published: August 2022

AbstractMonitoring vegetation restoration is challenging because monitoring is costly, requires long‐term funding, and involves monitoring multiple vegetation variables that are often not linked back to learning about progress toward objectives. There is a clear need for the development of targeted monitoring programs that focus on a reduced set of variables that are tied to specific restoration objectives. In this paper, we present a method to progress the development of a targeted monitoring program, using a pre‐existing state‐and‐transition model. We (1) use field data to validate an expert‐derived classification of woodland vegetation states; (2) use these data to identify which variable(s) help differentiate woodland states; and (3) identify the target threshold (for the variable) that signifies if the desired transition has been achieved. The measured vegetation variables from each site in this study were good predictors of the different states. We show that by measuring only a few of these variables, it is possible to assign the vegetation state for a collection of sites, and monitor if and when a transition to another state has occurred. For this ecosystem and state‐and‐transition models, out of nine vegetation variables considered, the density of immature trees and percentage of exotic understory vegetation cover were the variables most frequently specified as effective to define a threshold or transition. We synthesize findings by presenting a decision tree that provides practical guidance for the development of targeted monitoring strategies for woodland vegetation.
Winter locations of red‐throated divers from geolocation and feather isotope signatures
Authors: Duckworth James , O'Brien Susan , Petersen Ib K. , Petersen Aevar , Benediktsson Guðmundur , Johnson Logan , Lehikoinen Petteri , Okill David , Väisänen Roni , Williams Jim , Williams Stuart , Daunt Francis , Green Jonathan A. 
Published: August 2022

AbstractMigratory species have geographically separate distributions during their annual cycle, and these areas can vary between populations and individuals. This can lead to differential stress levels being experienced across a species range. Gathering information on the areas used during the annual cycle of red‐throated divers (RTDs; Gavia stellata) has become an increasingly pressing issue, as they are a species of concern when considering the effects of disturbance from offshore wind farms and the associated ship traffic. Here, we use light‐based geolocator tags, deployed during the summer breeding season, to determine the non‐breeding winter location of RTDs from breeding locations in Scotland, Finland, and Iceland. We also use δ15N and δ13C isotope signatures, from feather samples, to link population‐level differences in areas used in the molt period to population‐level differences in isotope signatures. We found from geolocator data that RTDs from the three different breeding locations did not overlap in their winter distributions. Differences in isotope signatures suggested this spatial separation was also evident in the molting period, when geolocation data were unavailable. We also found that of the three populations, RTDs breeding in Iceland moved the shortest distance from their breeding grounds to their wintering grounds. In contrast, RTDs breeding in Finland moved the furthest, with a westward migration from the Baltic into the southern North Sea. Overall, these results suggest that RTDs breeding in Finland are likely to encounter anthropogenic activity during the winter period, where they currently overlap with areas of future planned developments. Icelandic and Scottish birds are less likely to be affected, due to less ship activity and few or no offshore wind farms in their wintering distributions. We also demonstrate that separating the three populations isotopically is possible and suggest further work to allocate breeding individuals to wintering areas based solely on feather samples.