bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality by Design Approach for Development and Validation of a RP-HPLC Method for Simultaneous Determination of Xipamide and Valsartan in Human Plasma
Authors: sebaiy mahmoud M. , El-Adl Sobhy M. , Baraka Mohamed M. , Hassan Amira A. , Elsayed Heba M. 
Published: February 2022

Abstract
A new rapid, simple and sensitive RP-HPLC method was developed through employing Quality by Design (QbD) approach for determination xipamide and valsartan in Human plasma. Fractional factorial design (FFD) was applied for screening of the four independent factors: pH, flow rate, detection wavelength, and % of MeOH. Analysis of variance (ANOVA) confirmed that flow rate and % of MeOH were only significant. The optimization of the chromatographic conditions was carried out through using central composite design (CCD). Method Analysis was achieved using BDS Hypersil C8 (250 × 4.6 mm, 5 µm) column and an isocratic mobile phase of MeOH and 0.05M KH2PO4 buffer pH 3 (64.5:35.5, v/v) at 1.2 mL/min flow rate with UV detection at 240nm and 10 µL injection volume. According to FDA guidelines, the method was then validated for the determination of the two drugs clinically in human plasma in respect of future pharmacokinetic and bioequivalence simulation studies. The standard curve was linear in the concentration range of 5-100 µg/mL for both drugs, with a correlation coefficients (R2) of 0.999. The average recoveries ranged from 99.89 to 100.03%. The proposed method showed good predictability and robustness.
Evaluation of Pulmonary Functions in Patients of Type 2 Diabetes Mellitus: a Cross-sectional Study
Authors: Mittal Swati , Jindal Manisha , Srivast Saurabh , Sinha Smriti 
Published: February 2022

Abstract
Background – Diabetes mellitus (DM) has been broadly recognized as syndrome of hyperglycemia leading to various macro and microvascular complications. The different physiological systems that have been identified as a target of these injurious effects of hyperglycemia are excretory system, ocular system, central nervous system and cardiovascular system. Till date not much focus has been given to respiratory system as a possible target for deleterious effect of hyperglycemia.Objective – To assess the pulmonary functions in subjects with type 2 diabetes mellitus (T2DM) and compare them with age and sex matched healthy controls. Methods – This study was conducted on one hundred and twenty-five patients of type 2 diabetes Mellitus and a comparative number of age and sex matched non-diabetic individuals (control group) who met the inclusion and exclusion criteria. RMS Helios 401 computerized spirometer was used to assess pulmonary functions.Result – Results showed significantly lower values of FVC, FEV1, FEF25-75% and MVV among diabetic subjects as compared to controls (<0.05).Conclusion – We found that pulmonary function parameters in diabetic subjects were consistently lower than in healthy controls.
MFOC-CliqueNet: A CliqueNet Based Optimal Combination of Multidimensional Features Classification Method for Large-Scale Laser Point Clouds
Authors: Wang Lei , Li Xiaonan , Zhang Zhiyong , He Yueshun 
Published: February 2022

Abstract
As large-scale laser 3D point clouds data contains massive and complex data, it faces great challenges in the automatic intelligent processing and classification of large-scale 3D point clouds. Aiming at the problem that 3D point clouds in complex scenes are self-occluded or occluded, which could affect the object classification accuracy, we propose a multi-dimensional feature optimization combination classification method named MFOC-CliqueNet based on CliqueNet for large-scale laser point clouds. The optimal combination matrix of multi-dimensional features is constructed by extracting the three-dimensional features and multi-directional two-dimensional features of three-dimensional point cloud. This is the first time that multi-dimensional optimal combination features are introduced into cyclic convolutional networks CliqueNet. It is important for large-scale three-dimensional point cloud classification. The experimental results show that the MFOC-CliqueNet framework can realize the latest level with fewer parameters. The classification accuracy of our method is 98.9% on the Large-Scale Scene Point Cloud Oakland dataset, which is better than other classification algorithms mentioned in this paper.
The Effects of Intermittent Fasting from Dawn to Sunset for 4 Consecutive Weeks on Spinal Cord Injury: A Randomized Controlled Trial
Authors: Xiang Wu , Wang Junyu , Ji Bingjin 
Published: February 2022

Abstract
Spinal cord injury (SCI) leads to the permanent loss or reduction of the function of movement and sensation, and no effective clinical treatment has been reported. Animal studies have shown that intermittent fasting (IF) has a neuroprotective effect on SCI, but it has not been used in clinical treatment of SCI. Intermittent fasting from dawn to sunset is a form of fasting during human activity, which may improve the dysfunction of patients with SCI. Therefore, this study is the first to explore the safety of intermittent fasting from dawn to sunset in the treatment of SCI. Twenty-six SCI participants were randomly divided into a control group or an IF group. The IF group was fasted for more than 14 hours a day, and the control group had normal diet and water. Changes in blood routine, liver function, renal function, electrolytes, plasma glucose, weight, body mass index (BMI), American Spinal Injury Association (ASIA) score and occurrence of adverse events were monitored before enrollment and at the end of IF intervention. The IF group developed hypoglycemia, hunger, weight loss, and intestinal dysfunction (nausea, abdominal discomfort, diarrhea). There were no significant differences in blood routine, liver function, renal function, electrolytes and ASIA scores before and after IF intervention, or compared with the control group. Compared with the control group, there was no difference in body weight and BMI before IF intervention, and weight loss after IF intervention, but there was no significant change in BMI. Moreover, there were significant differences in body weight and BMI before and after IF intervention. The plasma glucose of the IF group began to decrease from the 14th day, and there were significant differences in plasma glucose before and after IF intervention. These findings suggested that IF was safe and feasible for SCI participants.
Four novel genes associated with longevity found in Cane Corso purebred dogs
Authors: Korec Evžen , Ungrová Lenka , Hejnar Jiří , Grieblová Adéla 
Published: February 2022

Abstract
Background: Longevity-related genes have been found in several animal species as well as in humans. The goal of this study was to perform genetic analysis of long-lived Cane Corso dogs with the aim to find genes that are associated with longevity. Results: SNPs with particular nucleotides were significantly overrepresented in long-lived dogs in four genes, TDRP, MC2R, FBXO25 and FBXL21. In FBXL21, the longevity-associated SNP localises to the exon. In the FBXL21 protein, tryptophan in long-lived dogs replaced arginine present in reference dogs.Conclusions: Four SNPs associated with longevity in dogs were identified using GWAS. We conclude that genes TDRP, MC2R, FBXO25 and FBXL21 are associated with longevity in Cane Corso dogs.
A persistent neutrophil-associated immune signature characterises post-COVID19 pulmonary sequelae
Authors: George Peter , Reed Anna , Desai Sujal , Devaraj Anand , Faiez Tasnim Shahridan , Laverty Sarah , Liu Michael , Kamal Faisal , Man William , Kaul Sundeep , Singh Suveer , Lamb Georgia , Burgoyne Thomas , Pinto Andreia , Farne Hugo , Priya Alessia Dalla , Aswani Andrew , Patella Francesca , Borek Weronika , Dokal Arran , Xu Xiao-Ning , Kelleher Peter , Shah Anand , Singanayagam Aran 
Published: February 2022

Abstract
Persistent interstitial lung changes with associated symptoms occur in a proportion of individuals that have recovered from severe acute respiratory syndrome coronavirus-2 (SARS-COV-2) infection through unknown mechanisms. We studied individuals with severe COVID-19 longitudinally following recovery from acute illness. Subjects with interstitial lung changes at 3-6 months post-recovery had an upregulated neutrophil-associated immune signature including increased chemokines, proteases and markers of neutrophil extracellular traps detectable systemically. Similar pathways were enriched in the upper airway with a concomitant augmentation of antiviral type-I interferon signalling. Interaction analysis of the peripheral phosphoproteome identified enriched kinases critical for neutrophil inflammatory pathways. Repeat sampling indicated that full normalisation of radiological and functional changes has not yet been reached in many individuals by 12 months post-recovery. These data provide functional insight into mechanisms driving pulmonary sequelae of COVID-19 and provide a rational basis for development of future targeted approaches to prevent long-term complications.
Changes in the characteristics of dissolved organic matter released from sediment according to precipitation in the Namhan River, South Korea.
Authors: Oh Haeseong , Choi Jung Hyun 
Published: February 2022

Abstract
In this study, changes in the properties of dissolved organic matter (DOM) released from sediments into water layers were investigated. To analyze the spatial and temporal variation in dissolved organic carbon (DOC), sediment and bottom water samples were collected upstream of the Gangcheon, Yeoju and Ipo weirs of the Namhan River during the rainy and non-rainy seasons. Vials containing the sediment and bottom water samples were incubated for seven days at 20 ℃ in the dark. Thereafter, the DOC concentration of the supernatant was analyzed at 0, 3 and 7 days of incubation. The results show that the change of bottom water DOC concentration resulted from the DOC released from the sediments. The initial DOC was significantly correlated with precipitation (p = 0.0042) and residence time (p = 0.0473). Furthermore, fluorescence analysis revealed that the DOM contained a significant quantity of hydrophilic and low-molecular-weight (LMW) organic matter in non-rainy season, and higher levels of hydrophobic and high-molecular-weight (HMW) organic matter were observed during the rainy season.
Effects of Gravitational Field of a Topological Defect on Statistical Properties of Heavy Quarkonia.
Authors: LIKENE André Aimé ATANGANA , ZARMA ALI , Ongodo Dieudonné Nga , Ema'a Jean Marie Ema'a , Abiama Patrice Ele , Bolie Germain Hubert Ben 
Published: February 2022

Abstract
The aim of this paper is to determine the behavior and statistical properties of non-relativistic heavy quarkonia interacting with the extended Cornel potential within a space-time generated by a cosmic-string. We extend the cornel potential by adding the inverse square potential plus the quadratic potential. We have calculated the energy eigenvalues and the corresponding eigenstates using the extended Nikiforov-Uvarov (NU) method. Then the thermodynamic properties like partition function, entropy, free energy, mean energy and specific heat capacity are calculated within the space-time of a cosmic-string. In the next step, we investigate the influence of the cosmic-string parameter on quantum states of heavy quarkonia and their statistical properties.
Nightmares, Mindfulness And Lucid Dreaming
Authors: Tzioridou Sofia , Dresler Martin , Sandberg Kristian , Mueller Erik M. 
Published: February 2022

Abstract
A theoretical and empirical association between lucid dreaming and mindfulness, as well as lucid dreaming and nightmares has previously been observed; however, the relationship between nightmares and mindfulness has received surprisingly little attention. Here, we present the findings of two studies exploring the relation of nightmare frequency and distress with two components of mindfulness, termed presence and acceptance, as well as lucid dreaming. Study 1 (N=338) consisted of a low percentage of frequent lucid dreamers whereas Study 2 (N=187) consisted primarily of frequent lucid dreamers that used lucid dream induction training techniques and meditation. Mindful acceptance showed a more robust association with nightmare-related variables in comparison to mindful presence. Meditation expertise inversely related to nightmare frequency and the practice of lucid dreaming induction techniques inversely related to nightmare frequency and distress. Finally, in Study 2 a positive correlation between lucid dreaming frequency and mindfulness was apparent. The present findings support the notion that wakeful mindfulness is associated with the quality of dreams and extend previous research by suggesting a disentangled role of the two facets of mindfulness in dream variation. This association remains open for experimental manipulation, the result of which could have clinical implications.
Enzyme Linked Immunosorbent Assays Based on Recombinant S1 and Its Truncated Proteins to Measure Porcine Serum IgA Antibody Against PEDV Could Reflect Neutralization Activity
Authors: Shan Ying , Gao Qin , Mao Junyong , Zheng Jingyou , Xu Xiaohan , Zhang Chuni , Huang Xiaojun , Xu Jidong , Shi Fushan , Yue Min , He Fang , Fang Weihuan , Li Xiaoliang 
Published: February 2022

Abstract
Porcine epidemic diarrhea virus (PEDV) can infect pigs of all ages, especially piglets. PEDV has spread across Asia since the 1980s. The highly virulent variant PEDV broke out on a large scale and caused huge economic losses to the pig industry in late 2010 in China. Rapid methods with high specificity are urgently needed for the diagnosis and control of the disease. In a previous study, we successfully established an ELISA method to detect porcine serum IgG antibody against PEDV based on full-length PEDV S1 protein as a coating antigen. In this study, the correlation between serum neutralizing activity and the value of IgA ELISA in porcine serum was further explored. First, we divided the PEDV S1 gene into three segments and constructed the recombinant plasmids pFastBac1-S1T1 (aa 21–279), pFastBac1-S1T2 (aa 280–539) and pFastBac1-S1T3 (aa 540–788), which carry the different antigenic regions of the S1 gene. Truncated S1 proteins PEDV-S1T1/S1T2/S1T3 were obtained by a Bac-to-Bac expression system, with protein sizes of 36 kDa, 38 kDa and 38 kDa, respectively. Recombinant proteins presented high reactivity with the monoclonal antibody against PEDV and positive pig serum. A total of 213 clinical serum samples were tested by the above indirect ELISA methods, and IFA was used as the gold standard. ROC curves revealed a significant correlation between S1-ELISA and S1T2-ELISA with a 0.9134 correlation coefficient and favourable sensitivity and specificity of S1-ELISA (93.24%, 95.68%) and S1T2-ELISA (89.33%, 94.16%). Our results also indicated that serum with higher neutralizing activity had a higher IgA titer based on S1-ELISA, S1T1-ELISA and S1T2-ELISA. In conclusion, both S1-ELISA and S1T2-ELISA can be used as candidate systems for detecting anti-PEDV IgA antibody titers in serum, which can reflect the level of neutralizing activity in pigs after natural infection or vaccination. The above research results provide a basis for the prevention and control of PEDV and can be used in the detection of host anti-infective immunity and evaluation of vaccine immune effects.
Modeling COVID-19 Effects on Sustainable Development Goals in Egypt Using System Dynamics
Authors: Marzouk Mohamed , Azab Shimaa , Elshaboury Nehal , Megahed Alaa , Metawie Mahmoud , Hawary Mostafa El , Ghaith Doaa , Bayoumi AbdElMoniem 
Published: February 2022

Abstract
The coronavirus disease 2019 (COVID-19) poses a significant threat to achieving the Sustainable Development Goals (SDGs). To address this challenge, a thorough examination of the pandemic’s influence on four SDGs in Egypt is presented in a system dynamic model. The addressed goals are related to no poverty (SDG 1), zero hunger (SDG 2), good health and well-being (SDG 3), decent work and economic growth (SDG 8), and climate action (SDG 13). The model is simulated over 35 years extending from 2015 to 2050. Furthermore, a web-based interactive learning environment is developed to analyze the interdependencies among public health activities and study the impacts of possible intervention countermeasures or prevention policies. This approach revitalizes debates about the achievement of SDGs amid the crisis and acts as a powerful tool that aids decision-makers in identifying leverage points to avoid the long-term negative repercussions of the crisis on the economy, people, and environment.
Type 2 Immune Response Plays A Critical Role in Trained Immunity
Authors: Cheng Shih-Chin , Dang Buyun , Zhang Jia , Gao Qingxiang , Zhong Qiumei , Zhang Lishan , Zhu Yanhui , Liu Junqiao , Niu Yujia , Xiao Nengming , Liu Wen-Hsien , Mao Kairui , Lin Shu-hai , Huang Jialiang , Huang Stanley , Ho Ping-Chih 
Published: February 2022

Abstract
Trained immunity, or innate immune memory, is vital in inducing heterologous protection against infection. Type 2 cytokines such as IL-4 and IL-13 induce M2 macrophage differentiation, characterized by anti-inflammatory phenotype permitting inflammation resolution and wound healing. Here we report that peritoneal macrophages isolated from OVA-allergen sensitized mice possessed both M2 signatures and a pro-inflammatory phenotype. Moreover, mice were more resistant to bacterial infection in the OVA-induced allergy model. Mechanistically, we found that IL-4 induced trained immunity in a STAT6-independent and IRS-dependent manner. Furthermore, IL-4 induces epigenetic memory in the pro-inflammatory gene enhancer regions, ensuring a higher transcription activity upon stimulation. In addition, IL-4 trained macrophages are metabolically more glycolytic upon LPS stimulation. To conclude, this unrecognized role of IL-4 in trained immunity broadens our understanding of IL-4 mediated physiological changes and provides immediate impacts on how type 2 immune response could re-direct disease progression in response to pathogen infection.
Why Batch Sensitization is Important for Missing Value Imputation
Authors: Sun Priscila Yun Qian , Goh Wilson Wen Bin 
Published: February 2022

Abstract
Data analysis is complex due to a myriad of technical problems. Amongst these, missing values and batch effects are particularly endemic. Although many methods have been developed for missing value imputation (MVI) and batch correction respectively, no study has directly considered the confounding impact of MVI on downstream batch correction. This is surprising as missing values are imputed during early pre-processing while batch effects are mitigated during late pre-processing, prior to functional analysis. The problem is that unless actively managed, MVI approaches generally ignore the batch covariate, with unknown consequences. We examine this problem by modelling 3 imputation strategies: global (M1), self-batch (M2) and cross-batch (M3) using simple matrix simulations, proteomics and genomics data. Considering batch covariates (M2) is important, resulting in enhanced batch correction and lower statistical errors. However, M1 and M3 are insidious: global and cross-batch averaging results in batch-effect dilution, with concomitant and irreversible increase in intra-sample noise. This noise is unremovable via batch correction algorithms and produces false positives and negatives. Hence, careless imputation in the presence of non-negligible covariates such as batch effects is costly.
Stiffness-Matched Extracellular Matrices Comprising Collagen or Fibrin Differentially Affect Cell-Mediated Remodeling and Peri-Cellular Stiffness
Authors: Jagiełło Alicja , Castillo Ulysses , Botvinick Elliot 
Published: February 2022

Abstract
Cells are known to continuously remodel their local extracellular matrix (ECM) and in a reciprocal way, they can also respond to mechanical and biochemical properties of their fibrous environment. In this study, we measured how stiffness around dermal fibroblasts (DFs) and human fibrosarcoma HT1080 cells differs with concentration of rat tail type 1 collagen (T1C) and type of ECM. Peri-cellular stiffness was probed in four directions using multi-axes optical tweezers active microrheology (AMR). First, we found that neither cell type significantly altered local stiffness landscape at different concentrations of T1C. Next, rat tail T1C, bovine skin T1C and fibrin cell-free hydrogels were polymerized at concentrations formulated to match median stiffness value. Each of these hydrogels exhibited distinct fiber architecture. Stiffness landscape and fibronectin secretion, but not nuclear/cytoplasmic YAP ratio differed with ECM type. Further, cell response to Y27632 or BB94 treatments, inhibiting cell contractility and activity of matrix metalloproteinases, respectively, was also dependent on ECM type. Given differential effect of tested ECMs on peri-cellular stiffness landscape, treatment effect and cell properties, this study underscores the need for peri-cellular and not bulk stiffness measurements in studies on cellular mechanotransduction.
Safe and low resistance solid-state high-voltage lithium metal batteries enabled by polyimide plastic crystal electrolytes
Authors: Liao Zhu , Hu Anyi , Huang Chenxi , Zhao Enyou , Saito Nagahiro , Zhang Zhengxi , Yang Li , Hirano Shin-ichi 
Published: February 2022

Abstract
Solid-state electrolytes (SSEs) have been attached widespread attention as next-generation materials as far as safety and energy density concerned, but they are severely plagued by the limited oxidative potential, poor physical contact with electrodes and lithium dendrite growth. Organic ionic plastic crystal (OIPC), solid analogues of ionic liquids (ILs), are intrinsic non-flammable, non-volatile and compatible with lithium anode. In the present work, an electrochemical durable polyimide plastic crystal electrolyte (PPCE, up to 5.5 V vs Li/Li+) can be obtained as a result of the good interaction between OIPC and Li salt. Also, the soft, deformable property of OIPC and proper melting point of OIPC-Li salt endure good physical contact with electrodes. Consequently, solid-state dendrite-free Li/PPCE/Li cells show an ultralong cycle life over 1000h under 1.0 mA cm− 2, and room temperature high-voltage Li/PPCE/ LiNi0.6Co0.2Mn0.2O2 cion cells achieve a capacity retention of 80% after 633 cycles under 1.0 C and ~ 5.0V class Li/PPCE/LiNi1.5Mn0.5O4 coins cells keep 91% discharge capacity after 100 cycles under 1.0 C at 25 ℃. The ionic and non-volatile nature of OIPC ensure the safety of PPCE so that it can past the harsh penetration test under practical conditions. Furthermore, the PPCE- based pouch cell shows good flexibility, acceptable rate performance and a high energy density of 223.1Wh kg− 1(based on merely electrolyte and electrodes). It is anticipated that our work provides an avenue for next-generation solid-state batteries.