bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of age inequalities in cause of death in the slow pace of epidemiological transition in India
Authors: Yadav Suryakant , Perianayagam Arokiasamy , Patel Shivani Anil , Cunningham Solveig Argeseanu 
Published: November 2022

AbstractIn developed countries, low disparity in lifespan contributed by the reduction in the burden of noncommunicable diseases (NCDs) is the key to advances in epidemiological transition. Contrarily, India passing through a phase of the dual burden of CDs and NCDs shows a heavy burden of NCDs responsible for the high disparity in lifespan. The Gini coefficient was decomposed for examining the contribution of 22 causes of death and their repercussions for inequality in age at death for 30 years between 1990–1994 and 2015–2019, using Global Burden of Disease data. The outcomes of the study reveal that India’s epidemiological transition has been just modest on account of high inequality in mortality by NCDs emplaced in the middle through old age despite a consistent mortality decline at infant through old age for communicable diseases (CDs). The structural changes in causes of death structure is shaped by CDs rather than NCDs, but overall bolstered by the adult mortality decline, especially in women. However, the process is restrained by the small contribution of the middle age group and a benign contribution of old mortality decline owing to the low threshold age. India needs to target health interventions in seeking significant mortality decline in the middle age group of 50–69 years that is warranted for epidemiological transition apace as evident in the developed nations.
Joint analysis of structured and semi-structured community science data improves precision of relative abundance but not trends in birds
Authors: Schindler Alexander R. , Cunningham Stephanie A. , Schafer Toryn L. J. , Sinnott Emily A. , Clements Sarah J. , DiDonato Frances M. , Mosloff Alisha R. , Walters Clay M. , Shipley Amy A. , Weegman Mitch D. , Zhao Qing 
Published: November 2022

AbstractEstimating absolute and relative abundance of wildlife populations is critical to addressing ecological questions and conservation needs, yet obtaining reliable estimates can be challenging because surveys are often limited spatially or temporally. Community science (i.e., citizen science) provides opportunities for semi-structured data collected by the public (e.g., eBird) to improve capacity of relative abundance estimation by complementing structured survey data collected by trained observers (e.g., North American breeding bird survey [BBS]). We developed two state-space models to estimate relative abundance and population trends: one using BBS data and the other jointly analyzing BBS and eBird data. We applied these models to seven bird species with diverse life history characteristics. Joint analysis of eBird and BBS data improved precision of mean and year-specific relative abundance estimates for all species, but the BBS-only model produced more precise trend estimates compared to the joint model for most species. The relative abundance estimates of the joint model were particularly more precise than the BBS-only estimates in areas where species detectability was low resulting from either low BBS survey effort or low abundance. These results suggest that community science data can be a valuable resource for cost-effective improvement in wildlife abundance estimation.
A single mutation in the E2 glycoprotein of hepatitis C virus broadens the claudin specificity for its infection
Authors: Shirasago Yoshitaka , Fukazawa Hidesuke , Nagase Shotaro , Shimizu Yoshimi , Mizukami Tomoharu , Wakita Takaji , Suzuki Tetsuro , Tani Hideki , Kondoh Masuo , Kuroda Takuya , Yasuda Satoshi , Sato Yoji , Hanada Kentaro , Fukasawa Masayoshi 
Published: November 2022

AbstractEntry of the hepatitis C virus (HCV) into host cells is a multistep process mediated by several host factors, including a tight junction protein claudin-1 (CLDN1). We repeatedly passaged HCV-JFH1-tau, an HCV substrain with higher infectivity, on Huh7.5.1-8 cells. A multi-passaged HCV-JFH1-tau lot was infectious to CLDN1-defective S7-A cells, non-permissive to original HCV-JFH1-tau infection. We identified a single mutation, M706L, in the E2 glycoprotein of the HCV-JFH1-tau lot as an essential mutation for infectivity to S7-A cells. The pseudovirus JFH1/M706L mutant could not infect human embryonic kidney 293 T (HEK293T) cells lacking CLDN family but infected HEK293T cells expressing CLDN1, CLDN6, or CLDN9. Thus, this mutant virus could utilize CLDN1, and other CLDN6 and CLDN9, making HCV possible to infect cells other than hepatocytes. iPS cells, one of the stem cells, do not express CLDN1 but express CLDN6 and other host factors required for HCV infection. We confirmed that the HCV-JFH1-tau-derived mutant with an M706L mutation infected iPS cells in a CLDN6-dependent manner. These results demonstrated that a missense mutation in E2 could broaden the CLDN member specificity for HCV infection. HCV may change its receptor requirement through a single amino acid mutation and infect non-hepatic cells.
Distributed time-varying out formation-containment tracking of multi-UAV systems based on finite-time event-triggered control
Authors: Cai Xin , Zhu Xiaozhou , Yao Wen 
Published: November 2022

AbstractConsidering the limited communication resources and slow convergence speed of multi-unmanned aerial vehicle (UAV) systems, this paper presents a finite-time even-triggered control framework for multi-UAV systems to achieve formation-containment tracking control. First, a virtual leader with time-varying output is introduced so that the trajectory of the whole system can be manipulated in real time. Second, the finite-time control enables that the systematic error converge to a small neighborhood of origin in finite time. Third, in order to save communication resources, an event-triggering function is developed to generate the control event sequences, which avoids continuous update of the controller. Rigorous proof shows the finite-time stability of the proposed control algorithm, and Zeno behavior is strictly excluded for each UAV. Finally, some numerical simulations are given to verify the effectiveness of the proposed controllers.
Exploring the effects of stationary camera spots on inferences drawn from real-time crash severity models
Authors: Abdi Amirhossein , Seyedabrishami Seyedehsan , Llorca Carlos , Moreno Ana Tsui 
Published: November 2022

AbstractThis study combined crash reports, land use, real-time traffic, and weather data to form an integrated database to analyze the severity of crashes taking place on rural highways. As the traffic cameras are placed at fixed locations, there is a wide range of measured distances between crashes and the selected nearest camera for extracting traffic variables. This may change the significance of traffic variables. For the first time, spacing was introduced as the distance around the detectors in which traffic characteristics are inferred to crashes. Classification and Regression Tree (CART) was employed as an interpretable tool to explore how spacing affects model performance and the significance of traffic variables. Twelve spacing scenarios from 250 to 3000 m were evaluated. Except for short spacings suffering from the low sample size issue, each model has a good predictive performance based on overall accuracy and F2 score in the 1000–3000 m spacings. In this range, three dominant rules emerged: (1) high deviations of speed on the roads surrounded by wastelands are associated with severe crashes; (2) faded markings in residential zones increase the likelihood of severe outcomes; (3) installation of barriers decrease the probability of severe crashes. Comparing the Variable Importance Measure (VIM) reveals that the total importance of traffic variables reduces as the spacing increases. Also, results indicate that average speed is significant until 1750 m; but speed deviation, traffic flow, and percent of heavy vehicles are more stable variables for further spacings. In conclusion, for the first time, spacing scenarios were evaluated systematically and proved that they have a remarkable impact on the significance of variables. This novel research provides guidance not only on the spacing but also on which real-time traffic variables have a greater impact on crash severity, along with design, land use, and environmental variables.
A medication-wide association study (MWAS) on repurposed drugs for COVID-19 with Pre-pandemic prescription medication exposure and pregnancy outcomes
Authors: Davidson Lena , Canelón Silvia P. , Boland Mary Regina 
Published: November 2022

AbstractInformation on effects of medication therapies during pregnancy is lacking as pregnant patients are often excluded from clinical trials. This retrospective study explores the potential of using electronic health record (EHR) data to inform safety profiles of repurposed COVID medication therapies on pregnancy outcomes using pre-COVID data. We conducted a medication-wide association study (MWAS) on prescription medication exposures during pregnancy and the risk of cesarean section, preterm birth, and stillbirth, using EHR data between 2010–2017 on deliveries at PennMedicine. Repurposed drugs studied for treatment of COVID-19 were extracted from ClinicalTrials.gov (n = 138). We adjusted for known comorbidities diagnosed within 2 years prior to birth. Using previously developed medication mapping and delivery-identification algorithms, we identified medication exposure in 2,830 of a total 63,334 deliveries; from 138 trials, we found 31 medications prescribed and included in our cohort. We found 21 (68%) of the 31 medications were not positively associated with increased risk of the outcomes examined. With caution, these medications warrant potential for inclusion of pregnant individuals in future studies, while drugs found to be associated with pregnancy outcomes require further investigation. MWAS facilitates hypothesis-driven evaluation of drug safety across all prescription medications, revealing potential drug candidates for further research.
Performance of the intracerebroventricularly injected streptozotocin Alzheimer’s disease model in a translationally relevant, aged and experienced rat population
Authors: Gáspár Attila , Hutka Barbara , Ernyey Aliz Judit , Tajti Brigitta Tekla , Varga Bence Tamás , Zádori Zoltán Sándor , Gyertyán István 
Published: November 2022

AbstractThe intracerebroventricularly (icv) injected streptozotocin (STZ) induced brain state is a widely used model of sporadic Alzheimer-disease (AD). However, data have been generated in young, naive albino rats. We postulate that the translationally most relevant animal population of an AD model should be that of aged rats with substantial learning history. The objective of the study was thus to probe the model in old rats with knowledge in various cognitive domains. Long-Evans rats of 23 and 10 months age with acquired knowledge in five-choice serial reaction time task (5-CSRTT), a cooperation task, Morris water-maze (MWM) and “pot-jumping” exercise were treated with 3 × 1.5 mg/kg icv. STZ and their performance were followed for 3 months in the above and additional behavioral assays. Both STZ-treated age groups showed significant impairment in the MWM (spatial learning) and novel object recognition test (recognition memory) but not in passive avoidance and fear conditioning paradigms (fear memory). In young STZ treated rats, significant differences were also found in the 5CSRTT (attention) and pot jumping test (procedural learning) while in old rats a significant increase in hippocampal phospho-tau/tau protein ratio was observed. No significant difference was found in the cooperation (social cognition) and pairwise discrimination (visual memory) assays and hippocampal β-amyloid levels. STZ treated old animals showed impulsivity-like behavior in several tests. Our results partly coincide with partly deviate from those published on young, albino, unexperienced rats. Beside the age, strain and experience level of the animals differences can also be attributed to the increased dose of STZ, and the applied food restriction regime. The observed cognitive and non-cognitive activity pattern of icv. STZ in aged experienced rats call for more extensive studies with the STZ model to further strengthen and specify its translational validity.
Modulation of intestinal epithelial cell proliferation and apoptosis by Lactobacillus gasseri SF1183
Authors: Di Luccia Blanda , Acampora Vittoria , Saggese Anella , Calabrò Viola , Vivo Maria , Angrisano Tiziana , Baccigalupi Loredana , Ricca Ezio , Pollice Alessandra 
Published: November 2022

AbstractThe gut microbiota exerts a variety of positive effects on the intestinal homeostasis, including the production of beneficial molecules, control of the epithelial barrier integrity and the regulation of the balance between host’s cell death and proliferation. The interactions between commensal bacteria and intestinal cells are still under-investigated and is then of paramount importance to address such interactions at the molecular and cellular levels. We report an in vitro analysis of the effects of molecules secreted by Lactobacillus gasseri SF1183 on HCT116 cells, selected as a model of intestinal epithelial cells. SF1183 is a L. gasseri strain isolated from an ileal biopsy of a human healthy volunteer, able to prevent colitis symptoms in vivo. Expanding previous findings, we show that bioactive molecules secreted by SF1183 reduce the proliferation of HCT116 cells in a reversible manner determining a variation in cell cycle markers (p21WAF, p53, cyclin D1) and resulting in the protection of HCT116 cells from TNF-alfa induced apoptosis, an effect potentially relevant for the protection of the epithelial barrier integrity and reconstitution of tissue homeostasis. Consistently, SF1183 secreted molecules increase the recruitment of occludin, a major component of TJ, at the cell–cell contacts, suggesting a reinforcement of the barrier function.
Interpretable brain disease classification and relevance-guided deep learning
Authors: Tinauer Christian , Heber Stefan , Pirpamer Lukas , Damulina Anna , Schmidt Reinhold , Stollberger Rudolf , Ropele Stefan , Langkammer Christian 
Published: November 2022

AbstractDeep neural networks are increasingly used for neurological disease classification by MRI, but the networks’ decisions are not easily interpretable by humans. Heat mapping by deep Taylor decomposition revealed that (potentially misleading) image features even outside of the brain tissue are crucial for the classifier’s decision. We propose a regularization technique to train convolutional neural network (CNN) classifiers utilizing relevance-guided heat maps calculated online during training. The method was applied using T1-weighted MR images from 128 subjects with Alzheimer’s disease (mean age = 71.9 ± 8.5 years) and 290 control subjects (mean age = 71.3 ± 6.4 years). The developed relevance-guided framework achieves higher classification accuracies than conventional CNNs but more importantly, it relies on less but more relevant and physiological plausible voxels within brain tissue. Additionally, preprocessing effects from skull stripping and registration are mitigated. With the interpretability of the decision mechanisms underlying CNNs, these results challenge the notion that unprocessed T1-weighted brain MR images in standard CNNs yield higher classification accuracy in Alzheimer’s disease than solely atrophy.
Determining epigenetic memory in kidney proximal tubule cell derived induced pluripotent stem cells using a quadruple transgenic reprogrammable mouse
Authors: Khelifi Gabriel , Chow Theresa , Whiteley Jennifer , Fort Victoire , Humphreys Benjamin D. , Hussein Samer M.I. , Rogers Ian M. 
Published: November 2022

AbstractThe majority of nucleated somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs). The process of reprogramming involves epigenetic remodelling to turn on pluripotency-associated genes and turn off lineage-specific genes. Some evidence shows that iPSCs retain epigenetic marks of their cell of origin and this “epigenetic memory” influences their differentiation potential, with a preference towards their cell of origin. Here, we reprogrammed proximal tubule cells (PTC) and tail tip fibroblasts (TTF), from a reprogrammable mouse to iPSCs and differentiated the iPSCs to renal progenitors to understand if epigenetic memory plays a role in renal differentiation. This model allowed us to eliminate experimental variability due to donor genetic differences and transfection of the reprogramming factors such as copy number and integration site. In this study we demonstrated that early passage PTC iPSCs and TTF iPSCs expressed low levels of renal progenitor genes and high levels of pluripotency-associated genes, and the transcriptional levels of these genes were not significantly different between PTC iPSCs and TTF iPSCs. We used ChIP-seq of H3K4me3, H3K27me3, H3K36me3 and global DNA methylation profiles of PTC iPSCs and TTF iPSCs to demonstrate that global epigenetic marks were not different between the cells from the two different sets of tissue samples. There were also no epigenetic differences observed when kidney developmental genes and pluripotency-associated genes were closely examined. We did observe that during differentiation to renal progenitor cells the PTC iPSC-derived renal cells expressed higher levels of three renal progenitor genes compared to progenitors derived from TTF iPSCs but the underlying DNA methylation and histone methylation patterns did not suggest an epigenetic memory basis for this.
Feasibility of a new bariatric fully endoscopic duodenal-jejunal bypass: a pilot study in adult obese pigs
Authors: Gonzalez Jean-Michel , Ouazzani Sohaib , Berdah Stephane , Cauche Nicolas , Delattre Cecilia , Peetermans Joyce A. , Santoro-Schulte Agostina , Gjata Ornela , Barthet Marc 
Published: November 2022

AbstractThis study assessed a new natural orifice transluminal endoscopic surgery (NOTES) bariatric endoscopic procedure in obese adult pigs. This 14-week prospective study compared 6 adult male Yucatan test pigs with baseline mean age 1.5 years to 2 control pigs. The test pigs received a fully endoscopic NOTES-based duodenal-jejunal bypass including measurement of the bypassed limb and creation of a gastrojejunal anastomosis (GJA) using a gastrojejunal lumen-apposing metal stent (GJ-LAMS) at Week 0, placement of a duodenal exclusion device (DED) at Week 2, and endoscopic examinations at Weeks 6 and 10. At Week 14, the pigs were sacrificed for necropsy. All endoscopic procedures were technically successful. By Week 14, the controls had gained a mean 1.1 ± 2.1 kg (+ 1.6%) while the intervention animals lost a mean 6.8 ± 3.9 kg (− 10.5%) since baseline. GJ-LAMS migrations occurred in 3 pigs, two of which also had DED migration and/or partial dislocation. Two other pigs died, one with aberrant splenic vein positioning near the GJA and the other from an unknown cause. An endoscopic bariatric bypass procedure with controlled bypass length was technically successful in all the cases and led to weight loss in test animals. Safety concerns must be further addressed.
Tapered self-expandable metallic stent optimized for Eustachian tube morphology in a porcine ET model
Authors: Kang Jeon Min , Kim Song Hee , Ryu Dae Sung , Park Yubeen , Won Dong-Sung , Kim Ji Won , Zeng Chu Hui , Kang Woo Seok , Park Jung-Hoon , Park Hong Ju 
Published: November 2022

AbstractSeveral investigations on the feasibility of stent placement into the Eustachian tube (ET) are being conducted. However, stents optimized for the anatomical structure of the ET have not yet been developed. In this study, the efficacy and safety of a self-expandable metallic stent (SEMS) optimized for porcine ET morphology was investigated. Silicone was injected into a cadaveric porcine ET to analyze the ET morphology. The three-dimensional-reconstructed porcine ET phantom images obtained after a computed tomography scan were measured to determine the dimensions of the porcine ET. The SEMS was designed as a tapered structure on the basis of the morphological findings of the porcine ET. The tapered SEMS (T-SEMS) and conventional SEMS (C-SEMS) were placed into the porcine ET to compare the safety and efficacy of the two types of SEMSs. Stent-induced tissue hyperplasia in the T-SEMS group was significantly lower than that in the C-SEMS group (p < 0.001). The T-SEMS optimized for the porcine ET was effective in maintaining stent patency. T-SEMS seems to be better than C-SEMS in suppressing stent-induced tissue hyperplasia, owing to the reduced stent-mediated mechanical injuries and maintaining ET patency.
Quality of glycemic control has significant impact on myocardial mechanics in type 1 diabetes mellitus
Authors: Hajdu Máté , Knutsen Maren Oedven , Vértes Vivien , Vorobcsuk-Varga Noémi , Molnár Gergő , Wittmann István , Faludi Réka 
Published: November 2022

AbstractThe potential associations between disease duration, glycemic control, and the echocardiographic markers of the myocardial mechanics were investigated in asymptomatic T1DM patients. Seventy T1DM patients (38.2 ± 11.7 years, 46 female) and 30 healthy volunteers were investigated. Besides the conventional and tissue Doppler measurements, left ventricular global longitudinal (GLS) and circumferential (GCS) strain as well as left and right atrial strain parameters were measured with 2D speckle tracking technique. Median HbA1c level was 7.4 (1.8)%. Even when added age and hypertension to the model, current HbA1c level remained independent predictor of left ventricular GLS (p = 0.002), GCS (p < 0.001), mitral e’ (p = 0.018), tricuspid e’ (p = 0.018) and left (p = 0.039) and right atrial conduit strain (p = 0.047) in multiple linear regression models. Correlations between disease duration and the echocardiographic variables lost their significance in multiple models. In patients with a combination of HbA1c ≤ 7.4% and no hypertension, echocardiographic findings did not differ from those in healthy volunteers. Patients with HbA1c > 7.4% and no hypertension and especially patients with coexisting hypertension and HbA1c > 7.4%, exhibited significantly impaired myocardial mechanics. Quality of glycemic control has a significant impact on myocardial mechanics in T1DM patients. Regarding disease duration this relationship was not proved.
Extensive archaeobotanical data estimate carrying capacity, duration, and land use of the Late Bronze Age settlement site Březnice (Czech Republic)
Authors: Šálková Tereza , Vobejda Libor , Chvojka Ondřej , Beneš Jaromír , Vondrovský Václav , Kuna Martin , Křivánek Roman , Menšík Petr , Novák Jan 
Published: November 2022

AbstractThe reconstruction of the settlement´s hinterland and acquisition of plant resources is one of the crucial questions in the field of environmental archaeology. Our study is focused on the reconstruction of the settlement’s structure and character of the environment from which the site drew resources. These research questions were addressed by the interpretation of plant macroremains, charcoals, and the results of the spatial model. We have focused on the maximum size of the settlement that the surrounding countryside was able to withstand. Our results clearly demonstrated significant deforestation and intensive land use in the vicinity of the Late Bronze Age study site. As the weed taxa showed, a wide range of crops was grown in rather dry or less often in damp fields. Based on our archaeobotanical results, we were able to reconstruct several types of grasslands: dry pastures and fallow fields on plateaus and slopes, wet pastures or meadows in the floodplain. Acidophilous oak forests, alluvial forests, and shrubs were reconstructed as the most common forest habitats in the vicinity of the study site. Based on the archaeological knowledge of the region, we assume relatively low population density during the Late Bronze Age, and thus only a small part of the more or less forested landscape was significantly affected by human activities.