bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of the file‐specific heat‐treated ProTaper Ultimate rotary system
Authors: Martins Jorge N. R. , Silva Emmanuel João Nogueira Leal , Marques Duarte , Ajuz Natasha , Rito Pereira Mário , Pereira da Costa Rui , Braz Fernandes Francisco Manuel , Versiani Marco Aurélio 
Published: December 2022

AbstractAimTo compare design, metallurgy and mechanical performance of the ProTaper (PT) Ultimate system with instruments of similar dimensions from the ProGlider, PT Gold and PT Universal systems.MethodologyNew PT Ultimate instruments (n = 248) were compared with instruments of similar dimensions from ProGlider (n = 31), PT Gold (n = 155) and PT Universal (n = 155) systems regarding their number of spirals, helical angle, blade symmetry, tip geometry, surface finishing, nickel/titanium ratio, phase transformation temperatures and mechanical performance. One‐way anova and nonparametric Mood's median tests were used for statistical comparison (α = 5%).ResultsAll instruments had symmetrical blades without radial lands or flat sides, similar surface finishing and an almost equiatomic nickel/titanium ratio, whilst the number of spirals, helical angles and the tip geometry were different. PT Ultimate instruments showed 3 distinct heat treatments that matched with the colour of their metal wire. Slider and ProGlider instruments had similar R‐phase start (Rs) and R‐phase finish (Rf) temperatures. SX, F1, F2, F3 and Shaper instruments showed equivalent heat treatments (Rs ~45.6°C and Rf ~28.3°C) that were similar to their PT Gold counterparts (Rs ~47.9°C and Rf ~28.2°C), but completely distinct to the PT Universal ones (Rs ~16.2°C and Rf ~−18.2°C). Amongst the PT Ultimate instruments, the lowest maximum torques were observed in the SX (0.44 N cm), Slider (0.45 N cm) and Shaper (0.60 N cm) instruments, whilst the highest was noted in the FXL (4.90 N cm). PT Ultimate Slider and ProGlider had similar torsional (~0.40 N cm) and bending loads (~145.0 gf) (p = 1.000), whilst the other PT Ultimate instruments showed statistically significantly lower maximum torque, higher angle of rotation and lower bending load (higher flexibility) than their counterparts of the PT Universal and PT Gold systems.ConclusionsThe PT Ultimate system comprises instruments with 3 distinct heat treatments that showed similar phase transformation temperatures to their heat‐treated analogues. PT Ultimate instruments presented lower torsional strength and superior flexibility than their counterparts, whilst maximum torque, angle of rotation and bending loads progressively increased with their sizes.
Ceramic tiles coated with Zr‐Ag co‐doped TiO2 thin film for indoor air purifying and antimicrobial applications
Authors: Taechasirivichai Kamonsasarat , Chiarakorn Siriluk , Chawengkijwanich Chamorn , Pongprayoon Thirawudh , Chuangchote Surawut 
Published: December 2022

AbstractIn this study, Zr‐Ag co‐doped TiO2 (ZAT) photocatalyst films having varied numbers of layers (1, 2, 3, and 4) have been developed to coat on ceramic tile substrates by sol‐gel spin coating technique. The specimens were tested to determine antibacterial activity against Escherichia coli and the capability to degrade gaseous formaldehyde under visible light. X‐ray diffraction, ultraviolet and visible absorption spectroscopy, water contact angle, and scanning electron microscopy were applied to characterize the structural and morphological properties of the samples. The photocatalytic reactivity of the nanocomposite films was investigated by the decolorization of methylene blue (MB) dye under visible light irradiation. The results showed that the two‐layer ZAT photocatalyst film on ceramic tile exhibited the highest photocatalytic decolorization of MB, with 60.36% efficiency. The ZAT tile had formaldehyde degradation efficiency up to 32.74% within only 6 h under visible light irradiation, higher than that of the bare ceramic tile (4.90%). Additionally, the ZAT thin films could enhance anti‐E. coli activity and could be capable of degrading air pollution.
Peri‐implant crevicular fluid and serum levels of soluble ST2 in peri‐implant diseases: A pilot study
Authors: Ozgur Engin , Topcu Deniz Ilhan , Bayraktar Nilufer , Alptekin Nilgun Ozlem 
Published: December 2022

AbstractBackground and ObjectiveSoluble ST2 (sST2) is a current biomarker of cardiovascular disease. It is used to predict susceptibility to cardiovascular diseases and to analyze their prognosis. Serum sST2 level increases in inflammatory diseases such as periodontitis. However, the level of sST2 in peri‐implant diseases and crevicular fluid has not been investigated yet. Thus, the aim of this cross‐sectional study is to analyze the level of sST2 in peri‐implant health and diseases.MethodsSixty‐nine participants were divided into 3 groups as peri‐implant health (PH), peri‐implant mucositis (PM), and peri‐implantitis (P‐I). Peri‐implant crevicular fluid (PICF) and serum samples were collected from each participant. The levels of sST2 and IL‐6 in PICF and sST2, IL‐6, and CRP in serum were compared between the groups. Pocket depth (PD), modified bleeding index (mBI), modified plaque index (mPI), keratinized mucosa index (KTW), and gingival/mucosal recession (REC) were recorded as clinical parameters. Biomarkers in the serum and PICF were analyzed by ELISA kit.ResultsSixty‐nine patients were included in the study. The differences in the following parameters were statistically significant between groups: age (p = .009), implant function time (p = .027), PD (p < .001), mBI (p < .001), mPI (p < .001), and KTW (p = .043). The PICF volume of P‐I and PM groups were statistically higher than PH (p < .001). The amount of sST2 in P‐I and PM groups were higher than PH (p = .043). Serum CRP was higher in the P‐I group than in other groups (p = .034). There were no significant differences in serum sST2 (p = .247) and IL‐6 (p = .110) levels between groups.ConclusionThe PICF levels of sST2 were significantly higher in PM and P‐I groups compared to the healthy group. However, no significant difference was observed between the groups in terms of serum sST2 level.
Dismantling and reorganizingPseudomonas marginalissensu lato
Authors: Sawada Hiroyuki , Fujikawa Takashi , Satou Mamoru 
Published: December 2022

AbstractUntil recently, it had been a common practice in the field of plant disease control to identify bacterial strains with the following characteristics asPseudomonas marginalis: fluorescent; oxidase‐, potato soft rot‐ and arginine dihydrolase‐positive; soft rot‐causing (here referred to asP.marginalissensu lato). In contrast, as phylogenetic and genomic analyses have been introduced into the bacterial taxonomy, it became clear thatP.marginalissensu lato is heterogeneous, suggesting that it might be a species complex containing many cryptic species. Therefore, a preliminary survey was performed targeting theP.marginalissensu lato strains preserved in the NARO (National Agriculture and Food Research Organization, Japan) Genebank to sort these into strains corresponding toP.marginalissensu stricto (i.e., strains that would be included in the same species range as the type strain ofP.marginalis) and strains that might not fit this category. Since then, attempts have been made to examine the taxonomic affiliations of the latter by using a polyphasic approach. As a result, some strains were proven to differ from any known species and are described as the following novel species:Pseudomonas kitaguniensis,Pseudomonas allii,Pseudomonas cyclaminis,Pseudomonas petroselini,Pseudomonas brassicae,Pseudomonas lactucae,Pseudomonas aegrilactucaeandPseudomonas morbosilactucae. In this article, the profiles of each of these novel species and a scheme for their identification are briefly introduced. In addition to the problem of cryptic species, reorganizingP.marginalissensu lato still faces various challenges, which we discuss.
Impact of rheumatoid arthritis and methotrexate on pregnancy outcomes: retrospective cohort study of the Italian Society for Rheumatology
Authors: Zanetti Anna , Zambon Antonella , Scirè Carlo Alberto , Bortoluzzi Alessandra 
Published: December 2022

ObjectiveTo evaluate the impact of rheumatoid arthritis (RA) and methotrexate (MTX) on the probability of becoming pregnant, pregnancy losses, elective termination of pregnancy (TOP) and congenital malformations.MethodsA retrospective cohort study on administrative healthcare databases was conducted. Three patients’ cohorts were enrolled among childbearing-age women. The first cohort included patients with RA starting MTX between July 2004 and December 2011. The second cohort included patients with RA without MTX treatment randomly selected from the same population (ratio 1:1). Finally, a cohort of subjects without RA was identified (ratio 1:4). Multivariate logistic regression models were implemented, ORs and 95% CI were reported.ResultsThe two matched RA cohorts included 3564 patients with MTX and without MTX. The cohort without RA included 14 256 subjects. In the three cohorts, the proportion of women achieving pregnancy during follow-up was 6.3%, 9.1% and 11.9%, respectively. Congenital malformations were very rare in all cohorts. RA women treated with MTX at any time before conception showed significantly higher risks of pregnancy losses than non-RA women (OR (95% CI) 2.22 (1.40 to 3.45)). We observed a significant positive relationship between the exposure to MTX in the 3 months window before conception and increased risk of elective TOP (OR (95% CI) 4.77 (1.08 to 19.40)).ConclusionMTX-treated patients appeared to be the cohort with the highest risk of pregnancy losses. The positive association with elective TOP and exposure to MTX in the three months window before conception in patients with RA reinforces the need for adequate preconception counselling to avoid unplanned pregnancies.
A 5-Pathway Signature Predicts Prognosis Based on Immune-Derived lncRNAs in Patients with Breast Cancer
Authors: Liu Bo , Zhu Nian , Huo Huixia , Long Junqi , Ji Xinchan , Li Jinmeng , Zhuang Xujie , Wang Huina , Li Lujia , Chen Yuhaoran , Yang Wei , Zhao Shuangtao 
Published: December 2022

Background. Currently, predictive models were not developed based on the signaling pathway signatures of immune-related lncRNAs in breast cancer (BRCA) patients. Methods. We selected unsupervised hierarchical clustering algorithm to classify patients with BRCA based on the significant immune-derived lncRNAs from the TCGA dataset. And different methods including ESTIMATE, ImmuneCellAI, and CIBERSORT were performed to evaluate the immune infiltration of tumor microenvironment. Using Lasso regression algorithm, we filtered the significant signaling pathways enriched by GSEA, GSVA, or PPI analysis to develop a prognostic model. And a nomogram integrated with clinical factors and significant pathways was constructed to predict the precise probability of overall survival (OS) of BRCA patients in the TCGA dataset (n = 1,098) and another two testing sets (n = 415). Results. BRCA patients were stratified into the PC (n = 571) and GC (n = 527) subgroup with significantly different prognosis with 550 immune-related lncRNAs in the TCGA dataset. Integrated analysis revealed different immune response, oncogenic signaling, and metabolic reprograming pathways between these two subgroups. And a 5-pathway signature could predict the prognosis of BRCA patients between these two subgroups independently in the TCGA dataset, which was confirmed in another two cohorts from the GEO dataset. In the TCGA dataset, 5-year OS rate was 78% (95% CI: 73–84) vs. 82% (95% CI: 77–87) for the PC and GC group (HR = 1.63 (95% CI: 1.17–2.28),

p
=
0.004

). The predictive power was similar in another two testing sets (HR > 1.20,

p

<

0.01

). Finally, a nomogram is developed for clinical application, which integrated this signature and age to accurately predict the survival probability in BRCA patients. Conclusion. This 5-pathway signature correlated with immune-derived lncRNAs was able to precisely predict the prognosis for patients with BRCA and provided a rich source characterizing immune-related lncRNAs and further informed strategies to target BRCA vulnerabilities.
ACTA2Expression Predicts Survival and Is Associated with Response to Immune Checkpoint Inhibitors in Gastric Cancer
Authors: Park Sunho , Karalis John D. , Hong Changjin , Clemenceau Jean R. , Porembka Matthew R. , Kim In-Ho , Lee Sung Hak , Wang Sam C. , Cheong Jae-Ho , Hwang Tae Hyun 
Published: December 2022

AbstractPurpose:We sought to identify biomarkers that predict overall survival (OS) and response to immune checkpoint inhibitors (ICI) for patients with gastric cancer.Experimental Design:This was a retrospective study of multiple independent cohorts of patients with gastric cancer. The association between tumor ACTA2 expression and OS and ICI response were determined in patients whose tumors were analyzed with bulk mRNA sequencing. Single-cell RNA sequencing (scRNA-seq) and digital spatial profiling data were used to compare tumors from patients with gastric cancer who did and did not respond to ICI.Results:Increasing tumor ACTA2 expression was independently associated with worse OS in a 567-patient discovery cohort [HR, 1.28 per unit increase; 95% confidence interval (CI), 1.02–1.62]. This finding was validated in three independent cohorts (n = 974; HR, 1.52 per unit increase; 95% CI, 1.34–1.73). Of the 108 patients treated with ICI, 56% of patients with low ACTA2 expression responded to ICI versus 25% of patients with high ACTA2 expression (P = 0.004). In an analysis of a publicly available scRNA-seq dataset of 5 microsatellite instability-high patients treated with ICI, the patient who responded to ICI had lower tumor stromal ACTA2 expression than the 4 nonresponders. Digital spatial profiling of tumor samples from 4 ICI responders and 5 ICI nonresponders revealed that responders may have lower ACTA2 expression in α-SMA–positive cancer-associated fibroblasts (CAF) than nonresponders (median: 5.00 vs. 5.50).Conclusions:ACTA2 expression is associated with survival and ICI response in patients with gastric cancer. ACTA2 expression in CAFs, but not in other cellular compartments, appears to be associated with ICI response.
Radiotherapy Induces Innate Immune Responses in Patients Treated for Prostate Cancers
Authors: Cheema Amrita K. , Li Yaoxiang , Ventimiglia Mary , Kowalczyk Keith , Hankins Ryan , Bandi Gaurav , Janowski Einsley-Marie , Grindrod Scott , Villagra Alejandro , Dritschilo Anatoly 
Published: December 2022

AbstractPurpose:Radiotherapy is a curative therapeutic modality used to treat cancers as a single agent or in combination with surgery and chemotherapy. Advanced radiotherapy technologies enable treatment with large fractions and highly conformal radiation doses to effect free-radical damage to cellular DNA leading to cell-cycle arrest, cell death, and innate immune response (IIR) stimulation.Experimental Design:To understand systemic clinical responses after radiation exposure, proteomic and metabolomic analyses were performed on plasma obtained from patients with cancer at intervals after prostate stereotactic body radiotherapy. Pathway and multivariate analyses were used to delineate molecular alterations following radiotherapy and its correlation with clinical outcomes.Results:DNA damage response increased within the first hour after treatment and returned to baseline by 1 month. IIR signaling also increased within 1 hour of treatment but persisted for up to 3 months thereafter. Furthermore, robust IIR and metabolite elevations, consistent with an early proinflammatory M1-mediated innate immune activation, were observed in patients in remission, whereas patients experiencing prostate serum antigen–determined disease progression demonstrated less robust immune responses and M2-mediated metabolite elevations.Conclusions:To our knowledge, these data are the first report of longitudinal proteomic and metabolomic molecular responses in patients after radiotherapy for cancers. The data supports innate immune activation as a critical clinical response of patients receiving radiotherapy for prostate cancer. Furthermore, we propose that the observed IIR may be generalized to the treatment of other cancer types, potentially informing multidisciplinary therapeutic strategies for cancer treatment.
Social impact of disease parameters and damage accrual in patients with juvenile-onset systemic Lupus Erythematosus: a multicenter cross-sectional study
Authors: Tanigava Nicolas Yamada , Sakamoto Ana Paula , Franco André Silva , Sales Lucas Peixoto , Aikawa Nadia Emi , Terreri Maria Teresa , Pereira Rosa Maria Rodrigues 
Published: December 2022

Objectives: To describe the frequency and investigate potential associations of unemployment, need of financial assistance and health-related quality of life in adult patients with Juvenile-onset Systemic Lupus Erythematosus (JSLE). Methods: In this multicenter cross-sectional study including JSLE adult patients, questionnaires were applied evaluating demographic characteristics, medical history, treatment, government financial assistance and work status, quality of life, economic classification, disease activity and damage accrual. Results: Sixty-nine JSLE patients from two Brazilian tertiary centers were included in this study. Twenty-eight (40.6%) patients were unemployed and 16 (23.2%) were receiving financial assistance or retirement. Work unemployment was associated only with higher damage scores (OR 1.95, 95% CI 1.14 to 3.35, p = 0.015), while the need of financial assistance was associated with higher damage scores (OR 1.83, 95% CI 1.02 to 3.27, p = 0.042), longer disease duration (OR 1.15, 95% CI 1.01 to 1.32, p = 0.043) and worse economic score (OR 0.87, 95% CI 0.76 to 0.99, p = 0.036). Emotional health, body image perception and pain were the most compromised domains of quality of life but showed no correlation with disease parameters. Disease activity, on the other hand, was inversely associated with symptoms scores (ρ = -0.34, p = 0.004) and scores of adverse effects of medications (ρ = -0.27, p = 0.024). Conclusion: JSLE is a disease with severe outcomes and high social burden that profoundly impact patients during adulthood. Damage accrual is the main risk factor for both unemployment and need of financial assistance and its prevention must be central in the management of JSLE patients.
Sense of mastery and attitude towards illness: Examining longitudinal benefits of a medical specialty camp for youth with sickle cell disease
Authors: Gillard Ann , Gagnon Ryan , Pashankar Farzana , Balsamo Lyn , Grafft Natalie , Miranda Jacqueline , Boruchov Donna , Neri Caitlin , Sprinz Philippa , Longyear Christine 
Published: December 2022

Medical specialty camps can play an important role in the positive development of psychosocial outcomes for children and youth with sickle cell disease (SCD). This study examined how sense of mastery and attitude towards illness outcomes changed over 6 months for 100 campers aged 8–16 years with SCD. The outcomes were measured twice before and twice after camp. Latent growth curve modeling was used to analyze data. Results showed no changes in the outcomes for this study population. Implications for future research designs, populations, and outcomes are discussed, as are implications for communications about camp, and policy and practice.
Null objects in Polish heritage language acquisition in contact with German
Authors: Sopata Aldona , Rinke Esther , Flores Cristina 
Published: December 2022

Aims and objectives: This study investigates the acquisition of referential expressions for direct objects by child heritage speakers of Polish living in Germany. Our main research questions are how object expression develops in bilingual children and whether their path or pace of acquisition differs from monolingual children. Methodology: We investigate the use of referential expressions in an elicited production task. In all, 39 Polish-German bilingual children participated in the test. Data and analysis: We compare the data of four age groups of bilingual children – 3- to 4-year-olds, 5- to 6-year-olds, 7- to 8-year-olds, and 9- to 10-year-olds – to each other and to monolingual children at the respective ages. For the analysis of participants’ responses, we ran a generalized linear mixed model (GLMM) with a multinominal dependent variable. Findings: The results show that child heritage language (HL) speakers of Polish display knowledge of semantic and pragmatic constraints of object realization from early stages on. However, from age 5 and up to age 9 to 10, they still produce high rates of inappropriate null objects and show a deceleration in the development of this knowledge, compared to monolingual children. This protracted development is attributed to reduced input in the HL, mainly due to the enrolment in the majority language school. Originality: This study is the first to investigate the development of referential expressions for direct objects in child heritage speakers of Polish in the age span 3 to 10 years. Significance: The study relates the higher rates of null objects in the bilingual production to the varying degrees of exposure to the HL during language development. Deceleration in the pace of object acquisition by the HL speakers at the age of 5 to 6 years is attributed to a prolonged stage of acquisition of integrating rules of syntactic and pragmatic knowledge.
Missense VKOR mutants exhibit severe warfarin resistance but lack VKCFD via shifting to an aberrantly reduced state
Authors: Li Shuang , Sun Jie , Liu Shixuan , Zhou Fengbo , Gross Michael L. , Li Weikai 
Published: December 2022

AbstractMissense vitamin K epoxide reductase (VKOR) mutations in patients cause resistance to warfarin treatment but not abnormal bleeding due to defective VKOR activity. The underlying mechanism of these phenotypes remains unknown. Here we show that the redox state of these mutants is essential to their activity and warfarin resistance. Using a mass spectrometry-based footprinting method, we found that severe warfarin-resistant mutations change the VKOR active site to an aberrantly reduced state in cells. Molecular dynamics simulation based on our recent crystal structures of VKOR reveals that these mutations induce an artificial opening of the protein conformation that increases access of small molecules, enabling them to reduce the active site and generating constitutive activity uninhibited by warfarin. Increased activity also compensates for the weakened substrate binding caused by these mutations, thereby maintaining normal VKOR function. The uninhibited nature of severe resistance mutations suggests that patients showing signs of such mutations should be treated by alternative anticoagulation strategies.