bluuebunny/crossref_metadata_2025_split huggingface dataset:
Theileria annulata SVSP455 interacts with host HSP60
Authors: Li Zhi , Liu Junlong , Zhao Shuaiyang , Ma Quanying , Guo Zhihong , Liu Aihong , Li Youquan , Guan Guiquan , Luo Jianxun , Yin Hong 
Published: August 2022

Abstract
Background
Theileria annulata, a transforming parasite, invades bovine B cells, dendritic cells and macrophages, promoting the uncontrolled proliferation of these cells. This protozoan evolved intricate strategies to subvert host cell signaling pathways related to antiapoptotic signaling to enable survival and proliferation within the host cells. However, the molecular mechanisms of the cell transformation induced by T. annulata remain largely unclear. Although some studies have predicted that the subtelomere-encoded variable secreted protein (SVSP) family plays roles in host-parasite interactions, the evidence for this is limited.

Methods
In the present study, the SVSP455 (TA05545) gene, a member of the SVSP gene family, was used as the target molecule. The expression pattern of SVSP455 in different life-cycle stages of T. annulata infection was explored using a quantitative real-time PCR assay, and the subcellular distribution of SVSP455 was observed using confocal microscopy. The host cell proteins interacting with SVSP455 were screened using the Y2H system, and their interactions were verified in vivo and in vitro using both bimolecular fluorescence complementation and confocal microscopy, and co-immunoprecipitation assays. The role played by SVSP455 in cell transformation was further explored by using overexpression, RNA interference and drug treatment experiments.

Results
The highest level of the SVSP455 transcript was detected in the schizont stage of T. annulata, and the protein was located both on the surface of schizonts and in the host cell cytoplasm. In addition, the interaction between SVSP455 and heat shock protein 60 was shown in vitro, and their link may regulate host cell apoptosis in T. annulata-infected cells.

Conclusion
Our findings are the first to reveal that T. annulata-secreted SVSP455 molecule directly interacts with both exogenous and endogenous bovine HSP60 protein, and that the interaction of SVSP455-HSP60 may manipulate the host cell apoptosis signaling pathway. These results provide insights into cancer-like phenotypes underlying Theilera transformation and therapeutics for protection against other pathogens.

Graphical Abstract
The lipid rafts in cancer stem cell: a target to eradicate cancer
Authors: Zhang Shuo , Zhu Neng , Li Hong Fang , Gu Jia , Zhang Chan Juan , Liao Duan Fang , Qin Li 
Published: August 2022

AbstractCancer stem cells (CSCs) are a subpopulation of cancer cells with stem cell properties that sustain cancers, which may be responsible for cancer metastasis or recurrence. Lipid rafts are cholesterol- and sphingolipid-enriched microdomains in the plasma membrane that mediate various intracellular signaling. The occurrence and progression of cancer are closely related to lipid rafts. Emerging evidence indicates that lipid raft levels are significantly enriched in CSCs compared to cancer cells and that most CSC markers such as CD24, CD44, and CD133 are located in lipid rafts. Furthermore, lipid rafts play an essential role in CSCs, specifically in CSC self-renewal, epithelial-mesenchymal transition, drug resistance, and CSC niche. Therefore, lipid rafts are critical regulatory platforms for CSCs and promising therapeutic targets for cancer therapy.
Mapping research evidence on perinatal asphyxia within the sustainable development era in sub-Saharan Africa: a scoping review protocol
Authors: Mensah Michael K. , Tano Kofi Franky K. , Ansu-Mensah Monica , Bawontuo Vitalis , Kuupiel Desmond 
Published: August 2022

Abstract
Background
Approximately 45% of all under-five child deaths are among newborn infants, babies in their first 28 days of life, or the neonatal period every year in the World Health Organization (WHO) Africa Region. To facilitate the achievement of Sustainable Development Goal (SDG) 3.2, innovative interventions are needed to address this challenge. Thus, this scoping review aims to map research evidence on perinatal asphyxia among neonates in the WHO Africa Region.

Methods
This scoping review will be guided by the Arksey and O’Malley framework, Levac et al. recommendations, and the Joanna Briggs Institute checklist for scoping reviews. Relevant published literature will be searched using a combination of keywords, Boolean terms, and Medical Subject Headings in the following databases: PubMed, SCOPUS, CINAHL, and Web of Science from January 2016 onwards. We will further search the World Health Organization and government websites, as well as the reference list of included studies for potentially eligible studies. This scoping review will include research evidence involving countries in the WHO Africa Region, with a focus on the burden of perinatal asphyxia, contributory/associated factors of perinatal asphyxia, clinical interventions for perinatal asphyxia, and interventions/strategies for the prevention of perinatal asphyxia in the SDGs era. Two reviewers will independently sort the studies to include and exclude, guided by the eligibility criteria. Also, the data from the included studies will be extracted and any discrepancies resolved using a third reviewer. Thematic analysis will be conducted, and the findings reported using both qualitative tables and figures. The Preferred Reporting Items for Systematic Reviews and Meta-Analysis Extension for Scoping Review (PRISMA-ScR) will be followed to report this study’s results. Quality appraisal of the included studies will be done utilising the mixed methods appraisal tool version 2018.

Conclusion
This scoping review results may reveal research evidence gaps to inform future primary studies, systematic reviews and meta-analyses; and possibly contribute towards the realisation of the SDG 3.2 by countries in the WHO Africa Region. The finding of this review will be disseminated using multiple channels such as workshops, peer review publications, conferences, and social media.
The least-used key selection method for information retrieval in large-scale Cloud-based service repositories
Authors: Gu Jiayan , Anjum Ashiq , Wu Yan , Liu Lu , Panneerselvam John , Lu Yao , Yuan Bo 
Published: August 2022

AbstractAs the number of devices connected to the Internet of Things (IoT) increases significantly, it leads to an exponential growth in the number of services that need to be processed and stored in the large-scale Cloud-based service repositories. An efficient service indexing model is critical for service retrieval and management of large-scale Cloud-based service repositories. The multilevel index model is the state-of-art service indexing model in recent years to improve service discovery and combination. This paper aims to optimize the model to consider the impact of unequal appearing probability of service retrieval request parameters and service input parameters on service retrieval and service addition operations. The least-used key selection method has been proposed to narrow the search scope of service retrieval and reduce its time. The experimental results show that the proposed least-used key selection method improves the service retrieval efficiency significantly compared with the designated key selection method in the case of the unequal appearing probability of parameters in service retrieval requests under three indexing models.
Does the material well-being at schools successfully compensate for socioeconomic disadvantages? Analysis of resilient schools in Sweden
Authors: Siebecke Deborah Elin , Jarl Maria 
Published: August 2022

Abstract
Background
A variety of studies point to a deterioration of educational equity in Sweden and increasing school segregation with respect to achievement and socioeconomic composition. Some schools are resilient to socioeconomic disadvantages in their student body and demonstrate high levels of achievement. However, little attention has been given to these resilient schools. Material well-being, as one important dimension of student well-being, comprises the student’s home background and school resources. The relationship between home background and achievement is well-established but less literature includes school-level factors of material well-being. In comparing the material well-being at resilient, non-resilient, and more advantaged schools, this study aims at detecting possible patterns that may provide crucial information as to why some schools succeed better in compensating for disadvantages.

Methods
Using Swedish data from the Programme for International Student Assessment (PISA) from 2000 to 2018, the shares of resilient, non-resilient, and more advantaged school groups with different achievement levels were identified by using aggregated achievement and socioeconomic background measures. Making use of a well-being framework specifically designed for PISA data, the school groups were compared regarding their material well-being as measured by the perceived shortage of material resources and teachers, the percentage of teachers fully certified, the availability of computers, and extracurricular activities. This comparison of school groups was computed using the nonparametric Kruskal-Wallis test and a Bonferroni-adjusted pairwise comparison.

Results
The shares of resilient schools decreased considerably from 14% in 2000 to 3% in 2015. Yet, the comparison of the material well-being at resilient and other school groups led to mostly non-significant results. Overall, disadvantaged schools reported higher teacher shortages than advantaged schools, which indicates the need for a more compensatory allocation of (human) resources.

Conclusions
The study concluded that the landscape of resilient schools is under continuous change. As no patterns of significant differences between resilient and other school groups were found, the study shows no indication that the material well-being at school compensates for disadvantages in a school’s student body. The findings call for further research regarding changes in the presence of resilient schools and their possible relationship with school material well-being.
Practice of non-pharmaceutical interventions against COVID-19 and reduction of the risk of influenza-like illness: a cross-sectional population-based study
Authors: Youssef Dalal , Issa Ola , Kanso Maysaloun , Youssef Janet , Abou-Abbas Linda , Abboud Edmond 
Published: August 2022

Abstract
Introduction
While the widespread implementation of the non-pharmaceutical interventions was intended to contain the COVID-19 pandemic, such measures could be also effective in limiting the spread of other respiratory infections. This study aimed to examine the association between the implementation of personal protective measures and the occurrence of influenza-like illnesses (ILI) in the general population.

Methods
An online retrospective cross-sectional observational study was conducted in April 2021 to assess cases of ILI among Lebanese adults aged 18 years and above, from all Lebanese governorates during the 2020–2021 flu season. Data were collected using a convenience sampling method. In addition to their socio-demographic information, participants were asked about their frequency of implementing personal protective measures and if they have experienced symptoms of ILI in the previous 6 months. The overall score of the personal protective measures was computed. Multivariable logistic regression was performed to examine the association between participants’ level of adoption of personal protective measures against COVID-19 and the occurrence of ILI.

Results
Among the 1019 Lebanese adults participating in this study, 352 (34.54%) of them reported symptoms of ILI between October 2020 and March 2021. Lebanese adults who wore their facemasks frequently or always were less likely to suffer from symptoms of ILI than others who did not wear the mask (aOR = 0.452, 95% CI = 0.349–0.693, p < 0.001). Similarly, adults who adopt the following protective measures washing hands (aOR = 0.608, 95% CI = 0.524–0.922, p < 0.001), respecting cough etiquette (aOR = 0.763, 95% CI = 0.598–0.918, p < 0.001), disinfecting surface (aOR = 0.892, 95% CI = 0.632–0.911, p = 0.012), avoiding crowded places (aOR = 0.739, 95% CI = 0.688–0.903, p = 0.049), respecting physical distancing (aOR = 0.646, 95% CI = 0.482–0.833, p = 0.031) on a regular basis (frequently/always) were less likely to report symptoms of influenza-like illnesses when compared with those who did not adhere at all to these measures.

Conclusion
Our study highlighted the potential of personal protective measures against COVID-19 in reducing the transmission of respiratory infections such as ILI. Such findings might be invested during influenza season, particularly among groups at high risk of developing severe complications. Exploring trends detected by the national severe acute respiratory infection surveillance system is recommended to confirm the utility of these measures.
Prevalence and factors associated with COVID-19 vaccine acceptance among the general population in Asadabad, Iran: a cross-sectional study
Authors: Albatineh Ahmed Najeeb , Dalvand Pegah , Aslani Marzieh , Saritas Serdar , Baghi Vajiheh , Ghanei Gheshlagh Reza 
Published: August 2022

Abstract
Background
Vaccination can be an essential protective measure against Coronavirus disease 2019 (COVID-19) if well received by the public. Various factors affect the acceptance or refusal of vaccines. Several waves of COVID-19 caused much death in Iran. This study aimed to evaluate the acceptance of the COVID-19 vaccine in the general population of Asadabad in 2021.

Methods
In this cross-sectional study, 650 people from the general population of Asadabad with a mean age of 34.6 (SD = 15.1) years were selected and included. In addition to socio-economic and demographic data, data were collected using the COVID-19 fear scale. Univariate and multiple logistic regression models were used to investigate the relationship between the tendency to get the COVID-19 vaccine (the dependent variable) and other variables.

Results
About 42.3% of participants were reluctant to receive the available COVID-19 vaccines. After adjusting for several covariates, there was a significant relationship between willingness to get vaccinated and family history of COVID-19 infection (AOR = 1.86, 95% CI 1.06–3.27, p = 0.032), trust in healthcare workers (AOR = 2.07, 95% CI 1.13–3.79, p = 0.019), trust in existing vaccines (AOR = 3.94, 95% CI 2.15–7.23, p < 0.001), encouraging family members to get vaccinated (AOR = 7.6, 95% CI 4.12–14.01, p < 0.0001). Also, people infected with COVID-19 are less likely to accept vaccination (AOR = 0.55, 95% CI 0.33–0.93, p = 0.025). Also, a unit increase in the score of fear of getting the COVID-19 virus increased the odds of getting the COVID-19 vaccine by 6% (AOR = 1.06, 95% CI 1.02–1.10, p = 0.002).

Conclusion
The culture and context of different societies can affect the acceptance or refusal of the COVID-19 vaccine. Based on these characteristics and providing extensive education to the people, the health authorities in each community should build trust and better communicate all health information to clear any fear and remove all obstacles to increase willingness to get COVID-19 vaccination.
EQ-5D-3L and quality of life in total knee arthroplasty (TKA) patients: beyond the index scores
Authors: Parkin David William , Do Rego Bronwyn , Shaw Rhonda 
Published: August 2022

Abstract
Background
Many patient reported outcome measures (PROMs) generate single number index scores that summarise responses to different questions within a questionnaire. Although these are valuable as unambiguous indicators of ill-health and changes in health, further information can be gained from examining the questionnaire responses themselves. This has additional importance since the patients’ reports are those responses and not the index scores. This paper reanalyses data from two prospective multicentre clinical studies assessing patients’ quality of life before and after total knee arthroplasty (TKA) using the EQ-5D-3L. Patients who completed EQ-5D-3L questionnaires at 3 time periods were included from 4 countries (USA, UK, Australia, New Zealand) operated on by the same surgeons using two different knee replacement systems. Descriptive analyses included levels within EQ-5D dimensions and profiles (combinations of dimensions and levels) at baseline and follow-up, including changes over time and differences between implants. Regression analysis quantified the relationship between the profiles and the EQ VAS.

Results
Problems with mobility, usual activities and pain & discomfort, in that order, were most frequent for pre-operative patients. There were large improvements in every dimension after surgery, but the time that this was observed differed: for mobility, self-care and anxiety & depression, most of the improvement occurred during the first 10 months; for usual activities and pain & discomfort, over 11–22 months. Analysing profiles, 85% of patients experienced an unequivocal improvement, 5.9% had no change, 3.5% worsened and 5.5% a mixed change at 11–22 months follow-up. Anxiety & depression had the greatest impact on EQ VAS scores; while mobility and usual activities were also important; usual activities was particularly important post-surgery.

Conclusions
The value of analysing patients’ responses to PROMs questionnaires without using summary scores was confirmed. The results add further detail to existing knowledge about the health problems that TKA patients experience before surgery, improvements after surgery, residual problems that some have, and the time at which improvements are experienced. This was a small study, but its methods may be easily replicated in other studies that use the EQ-5D-3L. It may also be possible to adapt them for other PROMs.
A review on room-temperature self-healing polyurethane: synthesis, self-healing mechanism and application
Authors: Li Yupeng , Jin Yong , Fan Wuhou , Zhou Rong 
Published: August 2022

AbstractPolyurethanes have been widely used in many fields due to their remarkable features such as excellent mechanical strength, good abrasion resistance, toughness, low temperature flexibility, etc. In recent years, room-temperature self-healing polyurethanes have been attracting broad and growing interest because under mild conditions, room-temperature self-healing polyurethanes can repair damages, thereby extending their lifetimes and reducing maintenance costs. In this paper, the recent advances of room-temperature self-healing polyurethanes based on dynamic covalent bonds, noncovalent bonds and combined dual or triple dynamic bonds are reviewed, focusing on their synthesis methods and self-healing mechanisms, and their mechanical properties, healing efficiency and healing time are also described in detial. In addition, the latest applications of room-temperature self-healing polyurethanes in the fields of leather coatings, photoluminescence materials, flexible electronics and biomaterials are summarized. Finally, the current challenges and future development directions of the room-temprature self-healing polyurethanes are highlighted. Overall, this review is expected to provide a valuable reference for the prosperous development of room-temperature self-healing polyurethanes.Graphical abstract
Implications of mitochondrial DNA variants in pediatric B-cell acute lymphoblastic leukemia
Authors: Jain Ayushi , Katiyar Amit , Singh Ritika , Bakhshi Sameer , Singh Harpreet , Palanichamy Jayanth Kumar , Singh Archna 
Published: August 2022

Abstract
Background
Research on the role of variations in the mitochondrial genome in pathogenesis of acute lymphoblastic leukemia (ALL) has been unfolding at a rapid rate. Our laboratory has previously described higher number of copies of the mitochondrial genomes per cell in pediatric ALL patients as compared to the healthy controls. In the current study, we evaluated the pattern of mitochondrial genome variations in 20 de-novo pediatric B-ALL cases and seven controls. Quantitative real-time Polymerase Chain Reaction was used for estimation of mitochondrial genomes’ copy number in bone marrow samples of each ALL patient and peripheral blood samples of controls. The complete mitochondrial genomes of all samples were sequenced using the Illumina platform.

Results
Sequencing data analysis using multiple mitochondrial genome databases revealed 325 variants in all 27 samples, out of which 221 variants were previously known while 104 were unassigned, new variants. The 325 variants consisted of 7 loss-of-function variants, 131 synonymous variants, 75 missense variants, and 112 non-coding variants. New, missense variants (n = 21) were identified in genes encoding the electron transport chain complexes with most of them encoding ND4, ND5 of complex I. Missense and loss-of-function variants were found to be deleterious by many predictor databases of pathogenicity. MuTect2 identified true somatic variants present only in tumors between patient-sibling pairs and showed overlap with missense and loss-of-function variants. Online MtDNA-server showed heteroplasmic and homoplasmic variants in mitochondrial genome.

Conclusions
The data suggest that some of these variations might have a deleterious impact on the expression of mitochondrial encoded genes with a possible functional relevance in leukemia.
A review on interleukins (IL10 and IL17) as biomarkers for hepatitis C-associated oral lichen planus
Authors: Hirani Suraiya , Charania Amynah , Salim Sandhiya , Faheem Sanam 
Published: August 2022

AbstractBackgroundHepatitis C virus is a viral infection associated with autoimmune disorders. This virus has hepatic and extrahepatic manifestations. One of the extrahepatic manifestations associated with the hepatitis C virus includes oral lichen planus. Oral lichen planus is an autoimmune disorder mainly affecting the tongue and buccal mucosa. It clinically represents grayish-white striae bilaterally on the buccal mucosa. The pathogenesis involves the progression of the hepatitis C virus, and oral lichen planus affects T lymphocytes. Specific proteins and cytokines activate these T lymphocytes, which act as biomarkers to detect certain diseases. Interleukin 10 is an anti-inflammatory cytokine, whereas interleukin 17 is a pro-inflammatory cytokine. These cytokines have a pathophysiological role and act as biomarkers for many diseases. Therefore, this review article aims to establish the role of interleukin 10 and interleukin 17 as biomarkers for hepatitis C-associated oral lichen planus.ConclusionHepatitis C virus is an infectious disease that can lead to liver cirrhosis, and oral lichen planus is a premalignant lesion that can lead to oral carcinoma. As interleukin 10 lessens the immune pathologies and interleukin 17 mediates proinflammatory response, therefore, these biomarkers have a role in progression of these diseases.
Correct Aharonov-Bohm wave functions for half-integer flux
Authors: Das Siddhant 
Published: August 2022

Abstract
A missing angle-dependent prefactor in the wave functions for half-integer magnetic flux obtained in Aharonov Y. and Bohm D., Phys. Rev., 115 (1959) 485 is supplied, without which the result is not single-valued. No physical conclusions of the paper are affected.