bluuebunny/crossref_metadata_2025_split huggingface dataset:
AAV9-mediated functional screening for cardioprotective cytokines in Coxsackievirus-B3-induced myocarditis
Authors: Carai Paolo , Ruozi Giulia , Paye Alexandra , Debing Yannick , Bortolotti Francesca , Lecomte Julie , Zentilin Lorena , Jones Elizabeth A. V. , Giacca Mauro , Heymans Stephane 
Published: May 2022

AbstractViral myocarditis (VM) is an important cause of heart failure (HF) in children and adults. However, the molecular determinants involved in cardiac inflammation and cardiomyocyte necrosis remain poorly characterized, and cardioprotective molecules are currently missing. Here, we applied an in vivo method based on the functional selection (FunSel) of cardioprotective factors using AAV vectors for the unbiased identification of novel immunomodulatory molecules in a Coxsackievirus B3 (CVB3)-induced myocarditis mouse model. Two consecutive rounds of in vivo FunSel using an expression library of 60 cytokines were sufficient to identify five cardioprotective factors (IL9, IL3, IL4, IL13, IL15). The screening also revealed three cytokines (IL18, IL17b, and CCL11) that were counter-selected and likely to exert a detrimental effect. The pooled overexpression of the five most enriched cytokines using AAV9 vectors decreased inflammation and reduced cardiac dilatation, persisting at 1 month after treatment. Individual overexpression of IL9, the top ranking in our functional selection, markedly reduced cardiac inflammation and injury, concomitant with an increase of anti-inflammatory Th2-cells and a reduction of pro-inflammatory Th17- and Th22-cells at 14 days post-infection. AAV9-mediated FunSel cardiac screening identified IL9 and other four cytokines (IL3, IL4, IL13, and IL15) as cardioprotective factors in CVB3-induced VM in mice.
Innovative diagnostic device for thickness measurement of conveyor belts in horizontal transport
Authors: Kirjanów-Błażej Agata , Błażej Ryszard , Jurdziak Leszek , Kozłowski Tomasz , Rzeszowska Aleksandra 
Published: May 2022

AbstractDiagnostics of conveyor belts used in horizontal transport without the need to take the belt off the conveyor and test it in laboratory conditions is an important aspect in mining plants (Jurdziak et al., Adv Intell Syst Comput, 835:645–654, 2019). Current testing, and thus obtaining knowledge about the current thickness of the conveyor belt covers, allows for control accelerated changes. It also avoids emergency stoppages in the operation of the conveyor and enables economically justified planning of a break in its operation. The article presents the concept of the first in Poland mobile device for measuring the thickness of conveyor belts in motion, implemented as part of the NCBR project (No. 0227 / L-10/2018 [LIDER program, Transport Przemysłowy i Maszyny Robocze, 1(47)/2020, pp. 60–61]), and also presents the measurement results obtained thanks to the laboratory version of the device.
A comprehensive atlas of Aggrecan, Versican, Neurocan and Phosphacan expression across time in wildtype retina and in retinal degeneration
Authors: Matsuyama A. , Kalargyrou A. A. , Smith A. J. , Ali R. R. , Pearson R. A. 
Published: May 2022

AbstractAs photoreceptor cells die during retinal degeneration, the surrounding microenvironment undergoes significant changes that are increasingly recognized to play a prominent role in determining the efficacy of therapeutic interventions. Chondroitin Sulphate Proteoglycans (CSPGs) are a major component of the extracellular matrix that have been shown to inhibit neuronal regrowth and regeneration in the brain and spinal cord, but comparatively little is known about their expression in retinal degeneration. Here we provide a comprehensive atlas of the expression patterns of four individual CSPGs in three models of inherited retinal degeneration andwildtypemice. Inwildtypemice, Aggrecan presented a biphasic expression, while Neurocan and Phosphacan expression declined dramatically with time and Versican expression remained broadly constant. In degeneration, Aggrecan expression increased markedly inAipl1-/-andPde6brd1/rd1, while Versican showed regional increases in the periphery ofRho-/-mice. Conversely, Neurocan and Phosphacan broadly decrease with time in all models. Our data reveal significant heterogeneity in the expression of individual CSPGs. Moreover, there are striking differences in the expression patterns of specific CSPGs in the diseased retina, compared with those reported following injury elsewhere in the CNS. Better understanding of the distinct distributions of individual CSPGs will contribute to creating more permissive microenvironments for neuro-regeneration and repair.
First crystal structures of 1-deoxy-d-xylulose 5-phosphate synthase (DXPS) from Mycobacterium tuberculosis indicate a distinct mechanism of intermediate stabilization
Authors: Gierse Robin M. , Oerlemans Rick , Reddem Eswar R. , Gawriljuk Victor O. , Alhayek Alaa , Baitinger Dominik , Jakobi Harald , Laber Bernd , Lange Gudrun , Hirsch Anna K. H. , Groves Matthew R. 
Published: May 2022

AbstractThe development of drug resistance by Mycobacterium tuberculosis and other pathogenic bacteria emphasizes the need for new antibiotics. Unlike animals, most bacteria synthesize isoprenoid precursors through the MEP pathway. 1-Deoxy-d-xylulose 5-phosphate synthase (DXPS) catalyzes the first reaction of the MEP pathway and is an attractive target for the development of new antibiotics. We report here the successful use of a loop truncation to crystallize and solve the first DXPS structures of a pathogen, namely M. tuberculosis (MtDXPS). The main difference found to other DXPS structures is in the active site where a highly coordinated water was found, showing a new mechanism for the enamine-intermediate stabilization. Unlike other DXPS structures, a “fork-like” motif could be identified in the enamine structure, using a different residue for the interaction with the cofactor, potentially leading to a decrease in the stability of the intermediate. In addition, electron density suggesting a phosphate group could be found close to the active site, provides new evidence for the D-GAP binding site. These results provide the opportunity to improve or develop new inhibitors specific for MtDXPS through structure-based drug design.
Static and dynamic analyses of free-hinged-hinged-hinged-free beam in non-homogeneous gravitational field: application to gravity gradiometry
Authors: Veryaskin Alexey V. , Meyer Thomas J. 
Published: May 2022

AbstractThe first analytical evaluation of a free-hinged-hinged-hinged-free beam proposed for use as the primary sensing element of a new gravity gradiometer is presented. Results of the evaluation obtained in quadratures are applied to the beam’s structure, including locating the hinges that form the beam’s boundary conditions allowing only free rotations around its nodal axes. These are deliberately chosen to minimize the beam’s symmetric free ends deflections under the uniform body loading of gravity while simultaneously permitting the beam’s maximum possible mirror-symmetric free ends deflections owing to a gravity gradient distributed along its length. The flexible triple-hinged beam deformation from its nominal unloaded geometry is naturally elastically coupled throughout, including free ends, allowing synchronized mechanical displacement measurements at any deflection point. Some methods of manufacturing such sensing elements and their respective error mechanisms are also discussed and presented for the first time.
Pre-transplant infusion of donor leukocytes treated with extracorporeal photochemotherapy induces immune hypo-responsiveness and long-term allograft survival in murine models
Authors: Schneiderman Jennifer , Qiu Longhui , Yeap Xin Yi , Kang Xin , Zheng Feibo , Ye Junsheng , Xie Yan , Wang Jiao-Jing , Sambandam Yuvaraj , Mathew James , Li Lin , Leventhal Joseph , Edelson Richard L. , Zhang Zheng Jenny 
Published: May 2022

AbstractRecipients of solid organ transplantation (SOT) rely on life-long immunosuppression (IS), which is associated with significant side effects. Extracorporeal photochemotherapy (ECP) is a safe, existing cellular therapy used to treat transplant rejection by modulating the recipient’s own blood cells. We sought to induce donor-specific hypo-responsiveness of SOT recipients by infusing ECP-treated donor leukocytes prior to transplant. To this end, we utilized major histocompatibility complex mismatched rodent models of allogeneic cardiac, liver, and kidney transplantation to test this novel strategy. Leukocytes isolated from donor-matched spleens for ECP treatment (ECP-DL) were infused into transplant recipients seven days prior to SOT. Pre-transplant infusion of ECP-DL without additional IS was associated with prolonged graft survival in all models. This innovative approach promoted the production of tolerogenic dendritic cells and regulatory T-cells with subsequent inhibition of T-cell priming and differentiation, along with a significant reduction of donor-specific T-cells in the spleen and grafts of treated animals. This new application of donor-type ECP-treated leukocytes provides insight into the mechanisms behind ECP-induced immunoregulation and holds significant promise in the prevention of graft rejection and reduction in need of global immune suppressive therapy in patients following SOT.
Evaluation of MxOy/fucoidan hybrid system and their application in lipase immobilization process
Authors: Kołodziejczak-Radzimska Agnieszka , Bielejewski Michał , Biadasz Andrzej , Jesionowski Teofil 
Published: May 2022

AbstractIn this work, new MxOy/fucoidan hybrid systems were fabricated and applied in lipase immobilization. Magnesium (MgO) and zirconium (ZrO2) oxides were used as MxOy inorganic matrices. In the first step, the proposed oxides were functionalized with fucoidan from Fucus vesiculosus (Fuc). The obtained MgO/Fuc and ZrO2/Fuc hybrids were characterized by means of spectroscopic analyses, including Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and nuclear magnetic resonance. Additionally, thermogravimetric analysis was performed to determine the thermal stability of the hybrids. Based on the results, the mechanism of interaction between the oxide supports and fucoidan was also determined. Furthermore, the fabricated MxOy/fucoidan hybrid materials were used as supports for the immobilization of lipase from Aspergillus niger, and a model reaction (transformation of p-nitrophenyl palmitate to p-nitrophenol) was performed to determine the catalytic activity of the proposed biocatalytic system. In that reaction, the immobilized lipase exhibited high apparent and specific activity (145.5 U/gcatalyst and 1.58 U/mgenzyme for lipase immobilized on MgO/Fuc; 144.0 U/gcatalyst and 2.03 U/mgenzyme for lipase immobilized on ZrO2/Fuc). The immobilization efficiency was also confirmed using spectroscopic analyses (FTIR and XPS) and confocal microscopy.
Blood flow stagnation after treatment of a giant internal carotid artery aneurysm: a computed fluid dynamics analysis
Authors: Muraoka Shinsuke , Takagi Reiya , Araki Yoshio , Uda Kenji , Sumitomo Masaki , Okamoto Sho , Nishihori Masahiro , Izumi Takashi , Nakamura Masanori , Saito Ryuta 
Published: May 2022

AbstractBalloon test occlusion (BTO) is an angiographic test to evaluate ischemic tolerance after permanent occlusion of an internal carotid artery (ICA). BTO can simulate ischemia caused by parent artery occlusion and can be used to select a suitable bypass surgery using specific criteria. On the other hand, a postoperative thrombus can form despite proper case selection, optimal radiological evaluation, and an appropriate surgical strategy. Postoperative ischemic complications related to perforating branches are clinically significant. This simulation study aimed to analyze postoperative flow characteristics and elucidate the cause of ischemic complications related to the perforating branch using computational fluid dynamics (CFD). An unexpected postoperative thrombus formation related to the perforating branch occurred after treating a giant aneurysm in the cavernous portion of the ICA in a patient. Three-dimensional digital subtraction angiography was used to acquire flow data and set up the CFD simulation. The flow simulations were performed at various bypass flow rates. The CFD analysis indicated flow stagnation in the ICA only when surgical treatment using a low-flow bypass graft was performed. Thrombus formation may lead to ischemic complications related to the perforating branch, such as the anterior choroidal artery. BTO did not reflect the influence of bypass blood flow. Therefore, recognizing that blood flow stagnation may occur and comprehensively deciding on the surgical strategy by CFD analysis can be helpful to prevent ischemic complications in patients with giant aneurysms.
Design of effective self-powered SnS2/halide perovskite photo-detection system based on triboelectric nanogenerator by regarding circuit impedance
Authors: Shooshtari Leyla , Ghods Soheil , Mohammadpour Raheleh , Esfandiar Ali , Iraji zad Azam 
Published: May 2022

AbstractSelf-powered detectors based on triboelectric nanogenerators (TENG) have been considered because of their capability to convert ambient mechanical energy to electrical out-put signal, instead of conventional usage of electrochemical batteries as power sources. In this regard, the self-powered photodetectors have been designed through totally two lay out called passive and active circuit. in former model, impedance matching between the TENG and the resistance of the circuit’s elements is crucial, which is not investigated systematically till now. In this paper, a cost effective novel planar photodetector (PD) based on heterojunction of SnS2 sheets and Cs0.05(FA0.83 MA0.17)0.95Pb(I0.83Br0.17)3 three cationic lead iodide based perovskite (PVK) layer fabricated which powered by graphene oxide (GO) paper and Kapton based contact-separated TENG (CS-TENG). To achieve the high performance of this device, the proper range of the load resistances in the circuit regards to TENG’s characterization has been studied. In the next steps, the integrated self-powered photo-detection system was designed by applying Kapton/FTO and hand/FTO TENG, separately, in the proposed impedance matching circuit. The calculated D* of integrated self-powered SnS2/PVK supplied by tapping the Kapton and hand on FTO is 2.83 × 1010 and 1.10 × 1013 Jones under the 10 mW/cm2 of white light intensity, the investigations determine that for designing significate performance of self-powered PD supplied by TENG, the existence of the load resistance with the well match amount to the utilized TENG is crucial. Our results which can be generalized to other types of passive self-powered sensors, are substantial to both academia and industry concepts.
A comprehensive study on essential oil compositions, antioxidant, anticholinesterase and antityrosinase activities of three Iranian Artemisia species
Authors: Shahrivari Saba , Alizadeh Saeedeh , Ghassemi-Golezani Kazem , Aryakia Elyas 
Published: May 2022

AbstractArtemisiais one of the most diverse genera in the Asteraceae family. The genus is wildly distributed in Irano-Turanian habitats and includes 34 species in Iran. Here, for the first time the essential oil variability, antioxidants and anti-cholinesterase and anti-tyrosinase activities of extracts of threeArtemisiaspecies(A. tournefortiana, A. khorassanica, A. haussknechtii), from different regions of Iran were evaluated. Based on GC–MS analyses, 81.84% to 98.70% of the total oils were identified. Cluster analysis grouped the studied populations in three different chemotypes. The highest and the lowest essential oil contents were observed inA. khorassanicaandA. haussknechtiispecies, respectively. Camphor, en-in-dicycloether, 1,8-cineole and (Z)-β-farnesene were the dominant components of essential oil in investigated ecotypes. The results revealed that the total phenol content was higher inA. tournefortianacollected from Kerman andA. haussknechtiicollected from Chaharmahal and Bakhtiari. However, the lowest phenol content was recorded forA. haussknechtiicollected from Isfahan province. The highest flavonoids content was found inA. tournefortianacollected from West Azerbaijan andA. khorassanicacollected from North Khorasan. The highest FRAP antioxidant activity was observed inA. tournefortiana(Kerman) and the lower amount was inA. haussknechtiicollected from Kohgiluyeh and Boyer-Ahmad. The highest antioxidant activity by DPPH method was inA. khorassanicacollected from South Khorasan and the lowest activity was in Isfahan'sA. haussknechtii.The acetycholine esterase inhibitory activity was higher inA. tournefortianacollected from West Azerbaijan; and the lowest activity was inA. haussknechtiicollected from Chaharmahal and Bakhtiari province. The highest tyrosinase inhibitory activity was inA. khorassanicacollected from North Khorasan; and the lowest was inA. haussknechtiicollected from Chaharmahal and Bakhtiari.
Diagnostic accuracy of probe-based confocal laser endomicroscopy and tissue sampling by endoscopic retrograde cholangiopancreatography in indeterminate biliary strictures: a meta‑analysis
Authors: Mi Junjie , Han Xiaofang , Wang Rong , Ma Ruijun , Zhao Danyu 
Published: May 2022

AbstractProbe-based confocal laser endomicroscopy (pCLE), also known as optical biopsy, is a new endoscopic technique that provides real-time magnification of 1000 × microscopic tissue information to diagnose indeterminate biliary strictures. Tissue sampling by endoscopic retrograde cholangiopancreatography (ERCP) is routinely performed to evaluate indeterminate biliary strictures. To evaluate the accuracy of pCLE and tissue sampling by ERCP in the diagnosis of indeterminate biliary strictures, 18 articles were included from 2008 to 2021 through Embase, PubMed, Web of Science, and Cochrane library databases. The summary estimates for the pCLE diagnosis of indeterminate biliary strictures were: sensitivity 0.88 (95% confidence interval (CI), 0.84–0.91); specificity 0.79 (95% CI 0.74–0.83); and Diagnostic Odds Ratio (DOR) 24.63 (95% CI 15.76–38.48). The summary estimates for tissue sampling by ERCP diagnosis for indeterminate biliary strictures were: sensitivity 0.54 (95% CI 0.49–0.59); specificity 0.96 (95% CI 0.94–0.98); and DOR 11.31 (95% CI 3.90–32.82). The area under the sROC curve of pCLE diagnosis of indeterminate biliary strictures is 0.90 higher than 0.65 of tissue sampling by ERCP. The pCLE is a better approach than tissue sampling by ERCP for the diagnosis of indeterminate biliary strictures by providing real-time microscopic images of the bile ducts.
12-Hydroxyjasmonic acid glucoside causes leaf-folding of Samanea saman through ROS accumulation
Authors: Yang Gangqiang , Ishimaru Yasuhiro , Hoshino Shunji , Muraoka Yuki , Uozumi Nobuyuki , Ueda Minoru 
Published: May 2022

AbstractFoliar nyctinasty, a circadian rhythmic movement in plants, is common among leguminous plants and has been widely studied. Biological studies on nyctinasty have been conducted using Samanea saman as a model plant. It has been shown that the circadian rhythmic potassium flux from/into motor cells triggers cell shrinking/swelling to cause nyctinastic leaf-folding/opening movement in S. saman. Recently, 12-hydroxyjasmonic acid glucoside (JAG) was identified as an endogenous chemical factor causing leaf-folding of S. saman. Additionally, SPORK2 was identified as an outward-rectifying potassium channel that causes leaf-movement in the same plant. However, the molecular mechanism linking JAG and SPORK2 remains elusive. Here, we report that JAG induces leaf-folding through accumulation of reactive oxygen species in the extensor motor cells of S. saman, and this occurs independently of plant hormone signaling. Furthermore, we show that SPORK2 is indispensable for the JAG-triggered shrinkage of the motor cell. This is the first report on JAG, which is believed to be an inactivated/storage derivative of JA, acting as a bioactive metabolite in plant.
Fatigue: a frequent and biologically based phenomenon in newly diagnosed celiac disease
Authors: Skjellerudsveen Berit Mære , Omdal Roald , Hetta Anne Kristine , Kvaløy Jan Terje , Aabakken Lars , Skoie Inger Marie , Grimstad Tore 
Published: May 2022

AbstractFatigue is increasingly recognized as a major complaint in patients with chronic inflammatory and autoimmune diseases. Although fatigue is assumed to represent a significant problem in celiac disease, existing knowledge is scarce, and opinions are conflicting. This study aimed to investigate the prevalence and severity of fatigue in patients with newly diagnosed celiac disease and compare it with healthy control subjects. Ninety patients with newly diagnosed celiac disease were compared with 90 age- and sex-matched healthy subjects. The primary endpoints were fatigue severity as measured by: the fatigue Visual Analog Scale (fVAS), the Fatigue Severity Scale (FSS), and the inverted Vitality subscale of the MOS36 (SF-36vs). Higher scores indicate more severe fatigue. Clinically relevant fatigue was determined using predefined cut-off values. Secondary endpoints were the associations between fatigue, and sex, age, depression, pain, and selected biochemical variables. The median (IQR) fVAS-scores were 43.0 (18.0–64.5) in patients, and 9.0 (2.0–16.0) in the control group (p < 0.001); and the FSS scores 3.8 (2.0–4.8) in patients, and 1.4 (1.0–1.9) in control subjects (p < 0.001). Inverted SF-36vs scores had a mean (SD) value of 58.8 (23.6) in patients, and 29.7 (14.3) in healthy subjects (p < 0.001). The presence of clinically relevant fatigue ranged from 41 to 50% in patients. Increased fatigue severity was associated with female sex, younger age, and elevated pain and depression scores, but not with levels of selected biochemical variables, including hemoglobin. Fatigue is a severe and frequent phenomenon in patients with untreated celiac disease.
A robust immune-related lncRNA signature for the prognosis of human colorectal cancer
Authors: Zhu Gongmin , Pei Lijiao , Yang Fan , Zhang Chenliang 
Published: May 2022

Abstract
Background: Colorectal cancer (CRC) is one of the most prevalent malignant cancers worldwide. Immune-related long non-coding RNAs (IRlncRNAs) are proved to be essential in the development and progression of carcinoma. The purpose of the present study was to develop and validate a prognostic IRlncRNA signature for CRC patients.
Methods: Gene expression profiles of CRC samples were downloaded from The Cancer Genome Atlas (TCGA) database. Immune-related genes were obtained from the ImmPort database and were used to identify IRlncRNA by correlation analysis. Through LASSO Cox regression analyses, a prognostic signature was constructed. Functional enrichment analysis was performed by gene set enrichment analysis (GSEA). TIMER2.0 web server and tumor immune dysfunction and exclusion (TIDE) algorithm were employed to analyze the association between our model and tumor-infiltrating immune cells and immunotherapy response. The expression levels of IRlncRNAs in cell lines were detected by quantitative real-time PCR (qPCR).
Results: A 9-IRlncRNA signature was developed by a LASSO Cox proportional regression model. Based on the signature, CRC patients were divided into high- and low-risk groups with different prognoses. GSEA results indicated that patients in high-risk group were associated with cancer-related pathways. In addition, patients in low-risk group were found to have more infiltration of anti-tumor immune cells and might show a favorable response to immunotherapy. Finally, the result of qPCR revealed that most IRlncRNAs were differently expressed between normal and tumor cell lines.
Conclusion: The constructed 9-IRlncRNA signature has potential to predict the prognosis of CRC patients and may be helpful to guide personalized immunotherapy.