bluuebunny/crossref_metadata_2025_split huggingface dataset:
The TLK1-MK5 axis regulates motility, invasion, and metastasis of prostate cancer cells
Authors: Khalil Md Imtiaz , De Benedetti Arrigo 
Published: October 2022

ABSTRACTBackgroundMetastatic dissemination of prostate cancer (PCa) accounts for majority of PCa related deaths. However, the exact mechanism of PCa cell spread is still unknown. We uncovered a novel interaction between two unrelated promotility factors, tousled-like kinase 1 (TLK1) and MAPK-activated protein kinase 5 (MK5), which initiates a signaling cascade promoting metastasis. In PCa, TLK1-MK5 signaling might be crucial as androgen deprivation therapy (ADT) leads to increased expression of both TLK1 and MK5 in metastatic patients, but in this work, we directly investigated the motility, invasive, and metastatic capacity of PCa cells following impairment of the TLK1>MK5 axis.ResultsWe conducted scratch wound repair and 3D invasion assays with LNCaP and PC3 cells to determine if TLK1 and MK5 can regulate motility and invasion. Both genetic depletion and pharmacologic inhibition of TLK1 and MK5 resulted in reduced migration and invasion through a Matrigel plug. We further elucidated the potential mechanisms underlying these effects and found that that this is likely due to reorganization of the actin fibers at lamellipodia and the focal adhesions network, in conjunction with increased expression of some MMPs that can affect penetration through the ECM. PC3, a highly metastatic cell line when assayed in xenografts, was further tested in tail-vein injection/lung metastasis model, and we showed that following inoculation, treatment with GLPG (MK5 specific inhibitor) or J54 (TLK1 inhibitor) the resulting lung tumor nodules were greatly diminished in number, and for J54 also in size.ConclusionOur data support that TLK1-MK5 axis is functionally involved in driving PCa cell metastasis and clinical aggressiveness, hence, disruption of this axis may inhibit the metastatic capacity of PCa.SIMPLE SUMMARYRecent work by us and others has illustrated the critical importance of MK5/PRAK in the invasive and motility properties of several cancer cell lines and some mouse models. In our earlier work we also uncovered that TLK1 modulates the activity of MK5 by phosphorylating S354 and two additional sites (S160 and S386).. We have now expanded on the possible mechanisms of the TLK1>MK5 pro-motility and invasive activity, and report that this may be due to reorganization of the actin fibers at lamellipodia and the focal adhesions network, in conjunction with increased expression of some MMPs. Pharmacological or genetic manipulation of prostate cancer (PCa) cell lines, LNCaP and PC3, results in drastic loss of in vitro motility and invasive capacity of these cells concomitant with alterations of their general morphology and reorganization of the focal adhesions distribution. In addition, PC3 used in tail-vein experimental metastases studies show that the use of GLPG (MK5 inhibitor) or J54 (TLK1 inhibitor) results in a drastic reduction of metastatic lung nodules, macroscopically and histologically.
Interpolatory proper order decomposition of nonlinear transmission line circuits
Authors: Condon Marissa , Hayes Brendan 
Published: October 2022

Purpose
The paper is concerned with interpolatory proper orthogonal decomposition (IPOD) methods for nonlinear transmission line circuits. This paper aims to examine several factors that must be considered when applying such model reduction techniques to this kind of circuit.

Design/methodology/approach
Two types of POD will be implemented. In each case, the choice of the order of the reduced model and the order of the interpolation space shall be considered. The stability of the models shall be explored.

Findings
The results indicate that the order for the reduced model to obtain accurate results depends on the chosen method when considering nonlinear transmission lines. The results also indicate that the structure of the nonlinear transmission line is crucial for determining the stability of the reduced models.

Originality/value
The work compares two IPOD methods and discusses the issues involved in achieving an accurate and stable reduced-order model for a nonlinear transmission line.
Kapter: pursuing innovation and competitive advantage in the Mexican B2B ecosystem
Authors: Valdés-Cervantes Víctor H. , Reyes-Mercado Pável 
Published: October 2022

Learning outcomes
The learning outcomes are as follows: to assess the competitive position of a small business-to-business (B2B) company to remain competitive in a developing country; to identify and shape the business opportunity that represents serving small and medium enterprises that would lead to competitive positioning in the B2B environment; and to compare innovation and commercialization projects through strategic dimensions related to managing a technology company in a way that fosters business profitability and growth.

Case overview/Synopsis
Margarita Kaplun, founder and CEO of Kapter, a small company providing country-wide technical and consultant services on thermography to industrial hubs in Mexico, had positioned the company as a reputed provider. However, the company had experienced razor-thin margins. Margarita needs to decide whether they pursue the following grant to fund a project on technology development that will source the competitive position or pursue a commercial project that help them achieve a larger customer base to reap higher income.

Complexity academic level
The case is oriented for an undergraduate audience, attending entrepreneurship, innovation and industrial organization courses. It depicts issues of technology-based entrepreneurship and is suitable for students in business, management and engineering.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 11: Strategy.
Factors influencing the organisational capabilities of the public sector for implementation of building information modelling in construction projects
Authors: Yusuf Anthony Olukayode , Opawole Akintayo , Musa Nofiu Abiodun , Kadiri Dele Samuel , Ebunoluwa Esther Ilori 
Published: October 2022

PurposeThis study examined factors influencing the organisational capabilities of the public sector for building information modelling (BIM) implementation in construction projects with a view to enhancing the performance of public sector projects.Design/methodology/approachThe study adopted a quantitative descriptive analysis that was based on primary data. In total, 198 valid questionnaires obtained from construction professionals within the public sector provided primary quantitative data for the assessment. The respondents provided the responses on the factors which were identified through an in-depth synthesis of literature relating to organisational capabilities of the public sector. Data collected were analysed using descriptive and inferential statistics.FindingsThe findings established that the potential of the public sector to deploy BIM in construction projects is greatly influenced by varying degree of organisational capability attributes with bureaucratic culture (mean score, MS = 3.37), structural complexity (MS = 3.17), lack of skilled and trained staff (MS = 3.12), personnel stability (MS = 3.11), staff cooperation (MS = 3.09) and political constraint (MS = 3.07) ranked highest. Through factor analysis, these and other highly influential factors were grouped into eight components, namely management-related, policy-related, technical-related, attitude-related, work structure-related, work ethic-related, decision-related and feedback-related factors. This grouping reflects the various components of organisational capability attributes which the public sector needs to efficiently develop to benefit from project management paradigm introduced by BIM.Practical implicationsThis study provided information for improving specific capability attributes with respect to human and technical resources as well as other soft infrastructure to support BIM implementation on building projects by the public sector client. The study also serves as a guide for understanding BIM implementation by the public sector in similar socio-political and economic contexts.Originality/valueThis assessment indicates various degrees by which the organisational attributes of public sector have influenced the attributes' capability to implement BIM on construction projects. Thus, findings provide information on areas of improvement for better implementation of BIM by the public sector in project delivery.
The behavioural intention to use MOOCs by undergraduate students: incorporating TAM with TPB
Authors: Wang Kai , van Hemmen Stefan Felix , Criado Josep Rialp 
Published: October 2022

PurposeThe purpose of the study is to determine the impact that the crucial determinants have on the behavioural intention (BI) of undergraduates to adopt Massive Open Online Courses (MOOCs), addressing two perspectives that are students' technical experience and psychosocial factors.Design/methodology/approachThis is a questionnaire study, and all the respondents are undergraduates in Barcelona, Spain. A total of 188 students are selected as survey subjects. Data collection applies the five-point Likert scale, and the collected information refers to demographic information and constructs related to the technology acceptance model (TAM) and theory of planned behaviour (TPB). Confirmatory factor analysis (CFA) and partial least square (PLS) are used to examine the measurement model's convergent and discriminant validity and test the hypotheses implementing the bootstrapping method.FindingsThe results show perceived usefulness (PU) and perceived ease of use (PEOU) are influential in forming an attitude (ATT), and PU could directly impact BI significantly. Additionally, the mediating role of ATT in the relationships amongst PU, PEOU and BI are investigated. Besides, perceived behavioural control (PBC) is found a critical variable and significantly impacts BI, whereas subjective norms (SN) is not found statistically significant in explaining BI in this study.Originality/valueThis study has confirmed that ATT has a positive mediation effect on PU, PEOU and BI. This study has also confirmed the validity and model fit of the incorporated model of TAM and TPB, which sufficiently accounts for the planned behavioural process of undergraduates to accept a MOOC and contributes to validating literature of the incorporated model of TAM and TPB. Besides, this study provides MOOCs managers with practical suggestions. First, ease-of-use interfaces should be designed and intensified. Second, more channels should be explored to satisfy students' needs. Third, managers should intensify the leverage of the systems and introduce the free trial period to new learners. Fourth, a bonus mechanism could be adopted to motivate students. Last, manager should intensify the construction of oral advertisements amongst students.
Assessing the impact of corporate environmental irresponsibility on workplace deviant behavior of generation Z and millennials: a multigroup analysis
Authors: Abbasi Munir A. , Amran Azlan , Sahar Noor e 
Published: October 2022

Purpose
Drawing on expectancy violation theory, this study aims to assess the impact of corporate environmental irresponsibility (CEI) on workplace deviant behaviors (WDB) of Generation Z and Millennials through the mediation of moral outrage.

Design/methodology/approach
The data were collected from 328 nonmanagerial employees working in the refinery, petroleum and power distribution companies who have been convicted for committing environmental irresponsibility by a court of law. Multigroup analysis (MGA) was used to estimate the hypothesized relationships.

Findings
Results revealed that CEI affects WDBs positively. Moreover, the MGA results demonstrated that the deviant behavior of Generation Z in response to environmental irresponsibility is higher than of the Millennials.

Research limitations/implications
Theoretically, the findings implicate that harming the environment will cost organizational performance through deviant behaviors.

Practical implications
This study provides a new lens for the executive management that eliminating social irresponsibility is more important than incurring sustainability initiatives, especially from the new generation’s perspective.

Originality/value
The originality of this study is that it confirmed the impact of CEI on employees’ deviant behaviors; and extended the scope of expectancy violation theory to the field of human resources.
Perception of time in the online product customization process
Authors: Wang Yue , Mo Daniel Y. , Ma Hoi Lam 
Published: October 2022

PurposeMany e-commerce companies adopt a product customization platform offering various choices for customers to configure products to better satisfy their needs. However, a method to effectively measure customer satisfaction is lacking. This paper aims to investigate customers' perception of time in the online configuration process of customized products and seeks to propose time perception as the measurement of the effectiveness of online product customization.Design/methodology/approachAn online laptop customization system was used in an empirical experiment to collect respondents' answers in a set of research questions. Regression and correlation analysis were conducted to investigate the factors affecting customers' satisfaction as well as the relationships with time perception.FindingsThe experimental results reveal several factors in customers' perception of time during the online product customization process. First, customers tend to overestimate the amount of time spent in a short-duration task but underestimate the amount of time spent in a long-duration task. Second, customers' perceptions of time are significantly correlated with their satisfaction with the configured products, and perceived time is moderately correlated with their satisfaction with the configuration process. Third, the difficulty of customization tasks and customers' motivation to process information also significantly affect customers' perceptions of time.Originality/valueThis paper advances the research on time perception by developing a new relative segmentation-based method to estimate the subjective perception of time. This study also makes several contributions to product customization research: the authors fill a research gap in the field of product customization by incorporating customers' perceptions of time into the measurement of customer satisfaction and by identifying the significant relationships among customers' perception of time, the ease of task selection, the customers' motivation to process information, and customers' satisfaction with customized products. These results aid in the design of online product customization systems.
Luxury customer's motivations to adopt gamification
Authors: Jain Sheetal , Mishra Sita , Saxena Garima 
Published: October 2022

PurposeIn the recent past, gamification has been recognized as an effective tool for engaging customers. However, there is limited research to explain luxury consumers' motivations to adopt gamification. This research applies the service-dominant (S-D) logic theory to investigate the association between motivation to adopt gamification, customer engagement, and affective commitment.Design/methodology/approachData were collected through structured questionnaires from a sample of 242 millennial luxury consumers in India. Collected data were examined using AMOS 25 and Process Macro for SPSS.FindingsThe findings reveal that consumers' social and personal integrative motives for gaming positively influence customer engagement with luxury brands. Further, all three sub-dimensions of customer engagement (conscious attention, enthused participation, and social connection) are also found to positively influence affective commitment. Customer engagement fully mediates the association between motivation and affective commitment, while the self-brand connection is found to weaken the mediated relationship.Originality/valueThis study is one of the first studies to explore luxury consumers' motivations behind engaging in gamification through moderated-mediation framework.
A multi‐data training method for a deep neural network to improve the separation effect of simultaneous‐source data
Authors: Wang Kunxi , Mao Weijian , Song Huan , Evinemi E. Isaac 
Published: October 2022

AbstractWithin the field of seismic data acquisition with active sources, the technique of acquiring simultaneous data, also known as blended data, offers operational advantages. The preferred processing of blended data starts with a step of deblending, that is separation of the data acquired by the different sources, to produce data that mimic data from a conventional seismic acquisition and can be effectively processed by standard methods. Recently, deep learning methods based on the deep neural network have been applied to the deblending task with promising results, in particular using an iterative approach. We propose an enhancement to deblending with an iterative deep neural network, whereby we modify the training stage of the deep neural network in order to achieve better performance through the iterations. We refer to the method that only uses the blended data as the input data as the general training method. Our new multi‐data training method allows the deep neural network to be trained by the data set with the input patches composed of blended data, noisy data with low amplitude crosstalk noise, and unblended data, which can improve the ability of the deep neural network to remove crosstalk noise and protect weak signal. Based on such an extended training data set, the multi‐data training method embedded in the iterative separation framework can result in different outputs at different iterations and converge to the best result in a shorter iteration number. Transfer learning can further improve the generalization and separation efficacy of our proposed method to deblend the simultaneous‐source data. Our proposed method is tested on two synthetic data and two field data to prove the effectiveness and superiority in the deblending of the simultaneous‐source data compared with the general training method, generic noise attenuation network and low‐rank matrix factorization methods.
Dietitians' perspectives on the role of dietetics practice in ‘gut health’
Authors: Williams Georgina M. , Tapsell Linda C. , Beck Eleanor J. 
Published: October 2022

AbstractAimsThis study aimed to explore dietitians' perspectives on the evidence surrounding the relationship between diet and ‘gut health’ and the current and emerging role of dietetics practice in this area.MethodsA qualitative descriptive methodology was used. Online semi‐structured interviews were conducted with Australian dietitians, focused on the perspectives related to gut health management in dietetics practice. Inductive thematic analysis was employed, commencing with initial coding by two researchers, and further coding leading to development of emergent themes. Divergent data were discussed and considered in analysis.ResultsFourteen interviews were conducted (2 males, 12 females). An overarching theme identified that current evidence is insufficient to direct dietetics practice change regarding gut health. Six subthemes on dietetics practice in ‘gut health’ emerged including (a) practice is multifaceted, (b) current practice aligns with dietary guidelines, (c) symptom management remains the primary concern, (d) evidence‐based information is sought, (e) translational evidence is required for practice change and (f) there is a role for dietetics in gut health research and translation.ConclusionsDietitians do not appear confident in their practice regarding gut microbiome‐related management and recognise there is currently limited translatable research to inform practice. Evidence to date suggests that recommendations for positive gut health do not differ substantially from Australian Dietary Guidelines. Dietitians will need additional education if further evidence emerges, however they demonstrated a strong commitment to evidence‐based practice.
Identifying rare variants inconsistent with identity‐by‐descent in population‐scale whole‐genome sequencing data
Authors: Johnson Kelsey E. , Adams Christopher J. , Voight Benjamin F. 
Published: October 2022

Abstract

Analyses of genetic variation typically assume that rare variants within a population are inherited from a single common ancestral event identity‐by‐descent (IBD). However, there are genetic and technical processes through which rare variants in population genetic data may deviate from this simple evolutionary model, including recurrent mutations, gene conversions and genotyping error. All these processes can decrease the expected length of shared background haplotype surrounding a rare variant if that variant was inherited from a single event descending from a common ancestor. No method exists to computationally infer rare variants inconsistent with this simple model—denoted here as ‘IBD‐inconsistent’—using unphased population sequencing data.

We hypothesized that the difference in shared haplotype background length can distinguish variants consistent and inconsistent with this simple IBD transmission population sequencing data without pedigree information. We implemented a Bayesian hierarchical model and used Gibbs sampling to estimate the posterior probability of IBD state for rare variants, using simulated recurrent mutations to demonstrate that our approach accurately distinguishes rare variants consistent and inconsistent with a simple IBD inheritance model.

Applying our method to whole‐genome sequencing data from 3,621 human individuals in the UK10K consortium, we found that IBD‐inconsistent variants correlated with higher local mutation rates and genomic features like replication timing. Using a heuristic to categorize IBD‐inconsistent variants as gene conversions, we found that potential gene conversions had expected properties such as enriched local GC content.

By identifying IBD‐inconsistent variants, we can better understand the spectrum of recent mutations in human populations, a source of genetic variation driving evolution and a key factor in understanding recent demographic history.