bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluorescence visualization of the neuropathic pain triad in trigeminal neuralgia
Authors: He Bin , Wang Wenlong , Zhang Runsen , Xu Yue , Wei Xiaoming , Yang Zhongmin , Cao Yang 
Published: November 2022

AbstractTrigeminal neuralgia (TN), an exemplary condition of neuropathic facial pain, seriously affects the physical and mental health of patients, becoming a major medical and social problem. So far, the mechanism of TN and its relation to neuronal activity remain unclear, largely limited by the spatial resolution of visualization methods. In the meanwhile, current therapeutic strategies targeting neurons have not achieved satisfactory outcome. Here, we investigate the neuropathic pain triad in TN by establishing an animal model of TN by chronic constriction injury of the unilateral infraorbital nerve (ION‐CCI) and leveraging the single‐cell resolution of confocal microscopy, including neuronal hyperexcitability, glial activation, and macrophage polarization. These results can broaden the understanding of TN pathogenesis from neurons to the neuropathic pain triad, and suggest that optical microscopy can provide new opportunities for understanding the complex pathogenesis of TN at single‐cell resolution, potentially contributing to the identification of more precise therapeutic targets and the development of more effective treatment modalities.
An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study
Authors: Mao Yong , Protzman Nicole M. , John Nikita , Kuehn Adam , Long Desiree , Sivalenka Raja , Junka Radoslaw A. , Shah Anish U. , Gosiewska Anna , Hariri Robert J. , Brigido Stephen A. 
Published: November 2022

AbstractAmniotic membrane (AM) is a naturally derived biomaterial with biological and mechanical properties important to Ophthalmology. The epithelial side of the AM promotes epithelialization, while the stromal side regulates inflammation. However, not all AMs are equal. AMs undergo different processing with resultant changes in cellular content and structure. This study evaluates the effects of sidedness and processing on human corneal epithelial cell (HCEC) activity, the effect of processing on HCEC inflammatory response, and then a case study is presented. Three differently processed, commercially available ocular AMs were selected: (1) Biovance®3L Ocular, a decellularized, dehydrated human AM (DDHAM), (2) AMBIO2®, a dehydrated human AM (DHAM), and (3) AmnioGraft®, a cryopreserved human AM (CHAM). HCECs were seeded onto the AMs and incubated for 1, 4 and 7 days. Cell adhesion and viability were evaluated using alamarBlue assay. HCEC migration was evaluated using a scratch wound assay. An inflammatory response was induced by TNF‐α treatment. The effect of AM on the expression of pro‐inflammatory genes in HCECs was compared using quantitative polymerase chain reaction (qPCR). Staining confirmed complete decellularization and the absence of nuclei in DDHAM. HCEC activity was best supported on the stromal side of DDHAM. Under inflammatory stimulation, DDHAM promoted a higher initial inflammatory response with a declining trend across time. Clinically, DDHAM was used to successfully treat anterior basement membrane dystrophy. Compared with DHAM and CHAM, DDHAM had significant positive effects on the cellular activities of HCECs in vitro, which may suggest greater ocular cell compatibility in vivo.
Osteoblastic STAT3 Is Crucial for Orthodontic Force Driving Alveolar Bone Remodeling and Tooth Movement
Authors: Gong Xinyi , Sun Siyuan , Yang Yiling , Huang Xiangru , Gao Xin , Jin Anting , Xu Hongyuan , Wang Xijun , Liu Yuanqi , Liu Jingyi , Dai Qinggang , Jiang Lingyong 
Published: November 2022

ABSTRACT
Mechanical force is essential to shape the internal architecture and external form of the skeleton by regulating the bone remodeling process. However, the underlying mechanism of how the bone responds to mechanical force remains elusive. Here, we generated both orthodontic tooth movement (OTM) model in vivo and a cyclic stretch-loading model in vitro to investigate biomechanical regulation of the alveolar bone. In this study, signal transducer and activator of transcription 3 (STAT3) was screened as one of the mechanosensitive proteins by protein array analysis of cyclic stretch-loaded bone mesenchymal stem cells (BMSCs) and was also proven to be activated in osteoblasts in response to the mechanical force during OTM. With an inducible osteoblast linage-specific Stat3 knockout model, we found that Stat3 deletion decelerated the OTM rate and reduced orthodontic force-induced bone remodeling, as indicated by both decreased bone resorption and formation. Both genetic deletion and pharmacological inhibition of STAT3 in BMSCs directly inhibited mechanical force-induced osteoblast differentiation and impaired osteoclast formation via osteoblast–osteoclast cross-talk under mechanical force loading. According to RNA-seq analysis of Stat3-deleted BMSCs under mechanical force, matrix metalloproteinase 3 (Mmp3) was screened and predicted to be a downstream target of STAT3. The luciferase and ChIP assays identified that Stat3 could bind to the Mmp3 promotor and upregulate its transcription activity. Furthermore, STAT3-inhibitor decelerated tooth movement through inhibition of the bone resorption activity, as well as MMP3 expression. In summary, our study identified the mechanosensitive characteristics of STAT3 in osteoblasts and highlighted its critical role in force-induced bone remodeling during orthodontic tooth movement via osteoblast–osteoclast cross-talk. © 2022 American Society for Bone and Mineral Research (ASBMR).
Triethanolamine‐modified montmorillonite clay as a new adsorbent for CO2 capture: Characterization, adsorption, and RSM modeling
Authors: Godarziani Hamed , Ramezanipour Penchah Hamid , Ghaemi Ahad 
Published: November 2022

AbstractIn this study, CO2 adsorption capacity was investigated by modified montmorillonite with triethanolamine due to its mechanical and chemical stability. The effect of operational parameters on CO2 adsorption capacity including temperature, pressure, and percentage triethanolamine was studied in the range of 30–70°C and 1–9 bar and 10–30%wt., respectively. Isothermal, kinetic, and thermodynamic analyses were performed by conventional method under equilibrium conditions. According to the R2 values from fitting the experimental data on the isothermal models, the Hill isotherm model was proposed for the unmodified and modified montmorillonite adsorbent. The results showed that the Elovich kinetic model provides the best fit for CO2 adsorption data for montmorillonite and triethanolamine‐modified montmorillonite. In the study of thermodynamic models, the positive slope of the Ln (Kd) diagram against 1/T indicates the thermal conductivity of the surface adsorption process. Optimal operating parameters were determined to obtain the highest adsorption capacity using the Design Expert software. For montmorillonite, the adsorption capacity obtained 219.86 mg/g at a temperature of 40°C and pressure of 7 bar, and for modified montmorillonite with triethanolamine, the optimal conditions for the input variables were 40°C, 7 bar, and 18.36%wt., respectively, and the adsorption capacity obtained was 248.55 mg/g.
Fibulin7 aggravates calcium oxalate‐induced acute kidney injury by binding to calcium oxalate crystals
Authors: Sugiura Hidekazu , Tsunezumi Jun , Yanagisawa Hiromi , Futaya Mayuko , Nitta Kosaku , Tsuchiya Ken 
Published: November 2022

AbstractFibulin7 (Fbln7) is a matricellular protein that is structurally similar to short fibulins but does not possess elastogenic abilities. Fbln7 is localized on the cell surface of the renal tubular epithelium in the adult kidney. We previously reported that Fbln7 binds artificial calcium phosphate particles in vitro, and that heparin counteracts this binding by releasing Fbln7 from the cell surface. Fbln7 gene (Fbln7) deletion in vivo decreased interstitial fibrosis and improved renal function in a high phosphate diet‐induced chronic kidney disease mouse model. However, the contribution of Fbln7 during acute injury response remains largely unknown. We hypothesized that Fbln7 serves as an exacerbating factor in acute kidney injury (AKI). We employed three AKI models in vivo and in vitro, including unilateral ureteral obstruction (UUO), cisplatin‐induced AKI, and calcium oxalate (CaOx)‐induced AKI. Here, we report that Fbln7KO mice were protected from kidney damage in a CaOx‐induced AKI model. Using HEK293T cells, we found that Fbln7 overexpression enhanced the CaOx‐induced upregulation of EGR1 and LAMB3, and that heparin treatment canceled this effect. Interestingly, the protective function observed in Fbln7KO kidneys was limited to the CaOx‐induced AKI model, while Fbln7KO mice were not protected against UUO‐induced renal fibrosis or cisplatin‐induced renal tubular damage. Taken together, our study indicates that Fbln7 mediates the local deposition of CaOx and damages the renal tubular epithelium. Releasing Fbln7 from the cell surface via heparin/heparin derivatives or Fbln7 inhibitory antibodies may provide a general strategy to mitigate calcium crystal‐induced kidney injuries.
Morphological structure of rat tongue using light and scanning electron microscopy
Authors: Demirci Beste , Kandil Banu , Yüksel Süleyman , Gültiken Murat Erdem 
Published: November 2022

AbstractThe rat is one of the most commonly used animals in biological research and experimental investigations in medicine. The ultrafine structural components of the epithelium differ depending on the anatomy of the animal and the papilla type. Animal adaptation to food types and environmental circumstances may also be linked to morphological diversity. In the current study, seven male Wistar rat tongues were investigated. For scanning electron microscope (SEM), two rat tongues were immersed in a 10% formalin solution and the other two rat tongues were immersed in a 2.5% glutaraldehyde solution. The tongues of three rats were fixed for regular histological evaluation using triple staining. The three primary components of the Wistar rat tongue are the apex, body and root. The apex had a rounded and bifurcated shape. Filiform papillae and gustatory papillae were easily identified on the dorsal side of the tongue. There were three forms of gustatory papillae; fungiform papillae, vallate papillae and foliate papillae. The purpose of this study was to expose the tongue morphology of the Wistar rat species, which is widely used in investigations. Also, we wanted to show that formalin fixation can be utilized for morphological research in SEM. Finally, the Wistar rat tongue was thoroughly investigated and compared to those of other species.
Ammonia transport is independent of PNH3 gradients across the gastrointestinal epithelia of the rainbow trout: A role for the stomach
Authors: Jung Ellen H. , Nguyen Jessica , Nelson Charlotte , Brauner Colin J. , Wood Chris M. 
Published: November 2022

AbstractAlthough the gastrointestinal tract (GIT) is an important site for nitrogen metabolism in teleosts, the mechanisms of ammonia absorption and transport remain to be elucidated. Both protein catabolism in the lumen and the metabolism of the GIT tissues produce ammonia which, in part, enters the portal blood through the anterior region of the GIT. The present study examined the possible roles of different GIT sections of rainbow trout (Oncorhynchus mykiss) in transporting ammonia in its unionized gas form—NH3—by changing the PNH3 gradient across GIT epithelia using in vitro gut sac preparations. We also surveyed messenger RNA expression patterns of three of the identified Rh proteins (Rhbg, Rhcg1, and Rhcg2) as potential NH3 transporters and NKCC as a potential ammonium ion (NH4+) transporter along the GIT of rainbow trout. We found that ammonia absorption is not dependent on the PNH3 gradient despite expression of Rhbg and Rhcg2 in the intestinal tissues, and Rhcg2 in the stomach. We detected no expression of Rhbg in the stomach and no expression of Rhcg1 in any GIT tissues. There was also a lack of correlation between ammonia transport and [NH4+] gradient despite NKCC expression in all GIT tissues. Regardless of PNH3 gradients, the stomach showed the greatest absorption and net tissue consumption of ammonia. Overall, our findings suggest nitrogen metabolism zonation of GIT, with stomach serving as an important site for the absorption, handling and transport of ammonia that is independent of the PNH3 gradient.
Preparation and characterization of zinc oxide nanoparticles supported on reduced graphene oxide and using as an effective catalyst for synthesis of l,4‐dihydropyrimidinones under solvent‐free conditions
Authors: Hemmesi Leila , Naeimi Hossein 
Published: November 2022

AbstractIn this research, it was presented a simple and easy method for the preparation of graphene oxide (GO) supported ZnO nanoparticles with low cost, available precursor and their application as nanocatalyst for synthesis of l,4‐dihydropyirimidinones. When GO combines with metal oxide nanoparticles such as ZnO shows very good properties. There is a wide range of chemical methods for the attachment of various functional groups. In this protocol, the GO was synthesized through Hummer's method. The structure and morphology properties of ZnO NPs@rGO nanocomposite were investigated by Fourier‐transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, and energy‐dispersive X‐ray spectroscope techniques. Also, the organic compounds were identified by melting point, FT‐IR, and proton nuclear magnetic resonance analyses.
Antibacterial activity against enterovirulent Escherichia coli strains from Bacillus amyloliquefaciens B31 and Bacillus subtilis subsp. subtilis C4: MALDI‐TOF MS profiling and MALDI TOF/TOF MS structural analysis on lipopeptides mixtures
Authors: Huarachi Sergio Fernando , Petroselli Gabriela , Erra‐Balsells Rosa , Audisio Marcela Carina 
Published: November 2022

AbstractIn this work, two Bacillus strains isolated from honey (Bacillus subtilis subsp. subtilis C4; access code HQ828992) and from a waste of an artisanal tannery (Bacillus amyloliquefaciens B31; access code KP893752) were evaluated in order to determine their antibacterial activity against five enteropathogenic Escherichia coli strains. The number of viable cultivable cells of the different strains of E. coli analyzed was determined by plate count. The crude cell‐free supernatants of both Bacillus strains exerted anti‐E. coli activities, whereas only the lipopeptide fraction of B31 had significant E. coli inhibition. The lipopeptides produced by the Bacillus were analyzed using matrix‐assisted laser desorption–ionization mass spectrometry (MALDI MS). The analysis was conducted combining the profiles (fingerprints) of the lipopeptides mixture and the individual lipopeptide fragmentation (tandem mass spectrometry [MS/MS] mode), both obtained from the same lipopeptides mixture sample, for higher output. Data obtained from C4 and B31 revealed that surfactin homologues were the most abundant lipopeptides produced by both strains studied. Additionally, kurstakin, iturin, and fengycin homologues were detected. Using the MS/MS mode, it was demonstrated that isobar compounds belonging to different families were produced by each Bacillus strain (e.g., C‐16 bacillomycin D was detected in B31 samples, meanwhile C‐15 iturin C was detected in C4). MS/MS analysis contributed with relevant information about the type of lipopeptides synthesized by Bacillus strains studied in this work.
Influencing factors of anti‐SARS‐CoV‐2‐spike‐IgG antibody titers in healthcare workers: A cross‐section study
Authors: Reusch Julia , Wagenhäuser Isabell , Gabel Alexander , Eggestein Annika , Höhn Anna , Lâm Thiên‐Trí , Frey Anna , Schubert‐Unkmeir Alexandra , Dölken Lars , Frantz Stefan , Kurzai Oliver , Vogel Ulrich , Krone Manuel , Petri Nils 
Published: November 2022

AbstractAgainst the background of the current COVID‐19 infection dynamics with its rapid spread of SARS‐CoV‐2 variants of concern (VOC), the immunity and the vaccine prevention of healthcare workers (HCWs) against SARS‐CoV‐2 continues to be of high importance. This observational cross‐section study assesses factors influencing the level of anti‐SARS‐CoV‐2‐spike IgG after SARS‐CoV‐2 infection or vaccination. One thousand seven hundred and fifty HCWs were recruited meeting the following inclusion criteria: age ≥18 years, PCR‐confirmed SARS‐CoV‐2 infection convalescence and/or at least one dose of COVID‐19 vaccination. anti‐SARS‐CoV‐2‐spike IgG titers were determined by SERION ELISA agile SARS‐CoV‐2 IgG. Mean anti‐SARS‐CoV‐2‐spike IgG levels increased significantly by number of COVID‐19 vaccinations (92.2 BAU/ml for single, 140.9 BAU/ml for twice and 1144.3 BAU/ml for threefold vaccination). Hybrid COVID‐19 immunized respondents (after infection and vaccination) had significantly higher antibody titers compared with convalescent only HCWs. Anti‐SARS‐CoV‐2‐spike IgG titers declined significantly with time after the second vaccination. Smoking and high age were associated with lower titers. Both recovered and vaccinated HCWs presented a predominantly good humoral immune response. Smoking and higher age limited the humoral SARS‐CoV‐2 immunity, adding to the risk of severe infections within this already health impaired collective.
Extended human papillomavirus genotype distribution in cervical intraepithelial neoplasia and cancer: Analysis of 40 352 cases from a large academic gynecologic center in China
Authors: Tang Xiao , Jones Terri E. , Jiang Wei , Austin Marshall , He Yanmei , Li Lei , Tong Lingling , Wang Cheng , Yang Kaixuan , Yin Rutie , Zhao Chengquan 
Published: November 2022

AbstractOur aim was to conduct a large epidemiologic analysis of the distribution of human papilloma virus (HPV) genotypes associated with cervical neoplasias and cancers at a major Chinese gynecologic center. The pathologic database was searched for cervical histopathologic diagnoses with prior HPV genotyping from liquid cervical cytology specimens obtained ≤6 months before biopsy. HPV testing was performed by using the Tellgenplex HPV27 or YanengBio HPV23 genotyping assays. A total of 40 352 cases meeting study criteria were identified. High risk human papillomavirus (hrHPV) was detected in 94.1% of squamous cancers compared to in only 83.3% of cervical adenocarcinomas. The prevalence of multiple HPV infections was highest in cervical intraepithelial neoplasia 1 (CIN1) (33.8%) and decreased with increasing severity of squamous lesions. The distribution of HPV genotypes was similar between CIN1 and histopathologic‐negative cases. HPV16 was one of the three most common hrHPV genotypes before all histopathologic abnormalities, ranging from 72.0% for cervical cancers, 38.7% for CIN2/3/AIS, 13.1% for CIN1, and 9.1% for biopsy‐negative cases. HPV16 and HPV18 accounted for over 87.2% of detected hrHPV genotypes for all glandular intraepithelial neoplastic lesions and cancers, whereas squamous lesions did not show this pattern. 80.3% of cervical cancers were associated with genotypes covered by HPV16/18 vaccines and 89.6% with genotypes covered by 9‐valent vaccination.
Assessment of immunomodulation and regulation of cell cycle in epithelium and stroma after Cidofovir injection in patients with recurrent respiratory papillomatosis—Pilot study
Authors: Solarz Paweł , Bodnar Magdalena , Czech Joanna , Mackiewicz–Nartowicz Hanna , Sinkiewicz Anna , Szylberg Łukasz , Borowczak Jędrzej , Rutkiewicz Paula , Zwierz Aleksander , Burduk Paweł 
Published: November 2022

AbstractRecurrent respiratory papillomatosis is strictly connected with human papillomavirus (HPV) infection of the epithelium of the upper respiratory tract. The main treatment of lesions located in the larynx or lower pharynx includes microsurgical excision by using a CO2 laser. To decrease the amount of surgical procedures gain in importance combined therapy with antiviral agents. The aim of this study was to investigate the effect of the intralesional application of Cidofovir on the tissue of laryngeal papillomas. We have shown that simultaneous microsurgery with adjuvant therapy of Cidofovir reduces chronic inflammation (by measuring the expression of CD4 and CD8 in tissue samples), cell proliferation, and regulates the cell cycle of HPV‐infected cells by reducing the expression of p53 and p63 proteins. In addition, this strategy reduces the multiple surgical procedures and regrowth of the pathology.
Acetabular coverage analysis of the proximal femoral head accurately characterizes dysplastic acetabular morphology
Authors: Nahal Chadi , Slullitel Pablo A. , Kamenaga Tomoyuki , Payne Emma R. , Nepple Jeffrey J. , Clohisy John C. , Pascual‐Garrido Cecilia 
Published: November 2022

AbstractThis study aimed to (1) measure acetabular sector angle (ASA) from proximal to distal positions along the axial femoral head axis, (2) identify acetabular deficiency patterns, and (3) correlate ASA at different axial positions with other radiological measurements in acetabular dysplasia. We identified 30 hips with dysplasia (lateral center edge angle [LCEA] <20°) and 30 hips without dysplasia (LCEA >25°) from a retrospective cohort. Anterior and posterior ASA (AASA, PASA) were measured in the axial computed tomography plane through the femoral head center (equatorial) and two axial positions above the equatorial line (intermediate and proximal). Deficiency patterns were identified using ASA cut‐off values determined from receiver operating characteristic curves. Pearson's coefficients were used for correlations. Compared to non‐dysplastic hips, AASA in dysplastic hips was significantly smaller in all levels: equatorial (46.1 ± 7.3 vs. 54.9 ± 8.5,° p < 0.001), intermediate (62.1 ± 11.2 vs. 69.0 ± 10.6,° p = 0.02), and proximal (102.9 ± 14.2 vs. 128.3 ± 23.0,° p < 0.001). According to proximal ASA (Pro‐ASA) cut‐off values in dysplastic hips, global deficiency was most prevalent (19/30, 63.3%), followed by anterior (6/30, 20%) and posterior (3/30, 10%) deficiency. There were strong correlations between acetabular anteversion and Eq‐AASA (r = −0.74, p < 0.001) and LCEA and pro‐PASA (r = 0.82, p < 0.001). Clinical significance: Acetabular sector angle provides insight into acetabular morphology and patterns of deficiency, providing essential information for precise acetabular reorientation.