bluuebunny/crossref_metadata_2025_split huggingface dataset:
Illuminating the G-Quadruplex: A Review on Fluorescent Probes for Detecting Polymorphic G-Quartet DNA Structures
Authors: Ganegamage Susantha K. , Heagy Michael D. 
Published: August 2022

Abstract:Modulating crucial biological processes such as gene regulation, aging, and relationship to globally important human health issues such as cancer has significantly brought considerable attention to G-quadruplex over the past few decades. As the impact of Gquadruplex emerges on so many biological roles, cancer prognosis and pathogenesis have not been fully understood, and selective small molecular binders with suitable chemical, photophysical and biological properties are potentially applicable biophysical tools for tracking Gquadruplex functions. The chemical properties include suitable water solubility, liphophilicity, etc., and the photophysical properties include excitation, emission, stoke-shift, lifetime, quantum yield, and measurable, selective changes of former photophysical parameters within the ideal spectral window upon interaction with the target. The biological properties include; toxicity, cellular infiltration, and selective binding with G-quadruplex over non-specific targets (e.g., duplex DNA, RNA, non-specific biomolecules etc.) in the complex cellular matrix. The development of G-quadruplex-selective probes, therefore, continues to be an important but challenging task for molecular therapeutic, diagnostic, imaging, and sensing applications. In this review, we have classified and summarized several classes of probes; carbocyanine, porphyrins, ethidium, carbazoles, acridines, tripodal or tetrapodal probes, pyrimidine carboxamides, tianguleniums, anthraquinones, polyaromatic hydrocarbons, BODIPY dyes, berberines, acetones and their derivatives for the variation of selectivity, photophysical, and biological properties with respect to the structural modifications, which ultimately provide helpful guidance for designing novel probes with optimal characteristics.
Atractylodes-I Overcomes the Oxidative Stress-induced Colonic
Mucosal Epithelial Cells Dysfunction to Prevent Irritable Bowel
Syndrome Via Modulating the miR-34a-5p-LDHA Signaling Pathway
Authors: Xu Ruilian , Liu Xianyong , Tian Mengfei , Chen Diping 
Published: August 2022

Background:
Irritable bowel syndrome (IBS) is a known brain-gut disorder. Currently, the molecular and cellular mechanisms of IBS remain unclear. Atractylenolide‐I (ATL-I) is majorly bioactive component extracted from Rhizoma Atractylodes Macrocephalae.

Methods:
Studies have revealed that ATL-I functioned as an anti-tumor drug in various
cancers. However, the effects and molecular mechanisms of ATL-I on the pathological
processes of colonic mucosal epithelial cells (CMECs) during IBS remain unclear. This
study reports ATL-I effectively alleviated the oxidative stress-induced colonic mucosal
epithelial cell dysfunction. In colonic mucosal tissues from IBS patients, we detected
upregulated miR-34a-5p and suppressed glucose metabolism enzyme expressions.
Under H2O2 treatment which mimics in vitro oxidative stress, miR-34a-5p was induced
and glucose metabolism was inhibited in the colon mucosal epithelial cell line, NCM460.
Meanwhile, ATL-I treatment effectively overcame the oxidative stress-induced miR-34a-
5p expression and glucose metabolism in NCM460 cells.

Result:
By bioinformatics analysis, Western blot and luciferase assay, we illustrated that
miR-34a-5p directly targeted the 3’UTR region of glucose metabolism key enzyme,
lactate dehydrogenase-A (LDHA) in colonic mucosal epithelial cells. Rescue
experiments validated that miR-34a-5p inhibited glucose metabolism by targeting LDHA.
Finally, we demonstrated that ATL-I treatment reversed the miR-34a-5p-inhibited
glucose metabolism and -exacerbated colonic mucosal epithelial cell dysfunction under
oxidative stress by modulating the miR-34a-5p-LDHA pathway.

Conclusion:
Summarily, our study reports the roles and mechanisms of ATL-I in the
oxidative stress-induced colonic mucosal epithelial cell dysfunction during IBS through
regulating the miR-34a-5p-LDHA-glucose metabolism axis.
The Emerging Role of Quercetin in the Treatment of Chronic Pain
Authors: Xu Ai-Jun , Zhou Ya-Qun , Liu Cheng , Liu Dai-Qiang , Tian Yu-Ke , Mei Wei , Tian Xue-Bi 
Published: August 2022

Abstract:Despite much research efforts being devoted to designing alternative pharmacological interventions, chronic pain remains to be an unresolved clinical problem. Quercetin, a compound that belongs to the flavonoids family, is abundantly found in fruits and vegetables. Emerging evidence indicates that quercetin possesses anti-nociceptive effects in different rodent models of chronic pain, including inflammatory pain, neuropathic pain and cancer pain. In this review, we summarize the mechanisms underlying the analgesic effect of quercetin in preclinical studies. These studies showed that quercetin exerts potent analgesic effects against chronic pain via suppressing neuroinflammation and oxidative stress as well as modulation of synaptic plasticity, GABAergic system, and opioidergic system. Considering that the safety of quercetin is well established, it has great potential for clinical use in pain treatment.
Targeted Therapies in Patients with Pulmonary Arterial Hypertension
Due to Congenital Heart Disease
Authors: Mares Adriana , Mukherjee Debabrata , Lange Richard A. , Nickel Nils P. 
Published: August 2022

Abstract:
Pulmonary arterial hypertension [PAH] is a devastating cardiovascular disease leading to right heart failure and death if untreated. Medical therapies for PAH have evolved substantially over the last decades and are associated with improvements in functional class, quality of life, and survival. PAH-targeted therapies now consist of multiple inhaled, oral, subcutaneous, and intravenous therapies targeting the phosphodiesterase, guanylate cyclase, endothelin and prostacyclin pathways.
Patients with congenital heart disease [CHD] are at high risk of developing PAH and growing evidence exists that PAH-targeted therapy can be beneficial in PAH-CHD. However, the PAH-CHD patient population is challenging to treat due to the heterogeneity and complexity of their cardiac lesions and associated comorbidities. Furthermore, most high-quality randomized placebo-controlled trials investigating the effects of PAH-targeted therapies only included a minority of PAH-CHD patients. Few randomized, controlled trials have investigated the effects of PAH-targeted therapy in pre-specified PAH-CHD populations. Consequently, the results of these clinical trials cannot be extrapolated broadly to the PAH-CHD population.
This review summarizes the data from high-quality clinical PAH treatment trials with a specific focus on the PAH-CAD?? population.
Repurposing Thalidomide, its Analogue and Apremilast for Possible Antiviral in Situation of Severe COVID Cytokine Syndrome
Authors: Thangaraju Pugazhenthan , Tanguturi Yella Sree Sudha , Lingareddygari Siva Sanker Reddy , Krishna Sasanka Kota Sesha Brahma Shree 
Published: August 2022

Background:COVID-19, caused by SARS-corona virus-2, is a globally expanded public health risk at a bizarre level. In this current situation, COVID-19 has become a serious emerging pandemic. Drug reusing is a crucial step in identifying the new uses of old established drugs. To achieve a significant and healthy way of treatment in COVID patients within a short duration, drug repurposing is a novel method.Objective:The present study concentrated on the molecular docking of thalidomide and its analogues and Apremilast against Coronavirus infectious symptoms, and evaluated virus proteins (Spike Protein, 3cl Protease, Nucleocapsids).Methods:The present study explores the possibility of repurposing thalidomide for the treatment of SARS-COV-2 infection by assessing and confirming with docking affinity scores of thalidomide and its analogues and Apremilast, with spike protein, 3cl protease, and nucleocapsids.Results:From the study results, thalidomide, pomalidomide, lenalidomide, and Apremilast exhibited better binding affinity to N Protein (4KXJ), Protease (4WY3) and Spike Protein (5WRG). In comparison to targets, N Protein - 4KXJ is the best for the four ligands. It is finalized that all four ligands (Thalidomide -8.6, Pomalidomide -8.8, Lenalidomide, and -8.2,and Apremilast -8.1) have good docking scores with the target N Protein.Conclusion:The present study confirms that thalidomide and its analogues and apremilast are a better fit for treating high risk patients of COVID-19 viral infection, which are supposed to promote beneficial effects for both respiratory illnesses like COVID-19 symptoms as well as improve the pathological state of condition.
In Schizophrenia, Chronic Fatigue Syndrome- and Fibromyalgia-Like
Symptoms are Driven by Breakdown of the Paracellular Pathway with
Increased Zonulin and Immune Activation-Associated Neurotoxicity
Authors: Maes Michael , Andrés-Rodríguez Laura , Vojdani Aristo , Sirivichayakul Sunee , Barbosa Decio S. , Kanchanatawan Buranee 
Published: August 2022

Background:
A meaningful part of schizophrenia patients suffer from physiosomatic symptoms
(formerly named psychosomatic), which are reminiscent of chronic fatigue syndrome and fibromyalgia
(FF) and are associated with signs of immune activation and increased levels of tryptophan
catabolites (TRYCATs).

Aims:
The study aims to examine whether FF symptoms in schizophrenia are associated with the
breakdown of the paracellular pathway, zonulin, lowered natural IgM responses to oxidative specific
epitopes (OSEs); and whether FF symptoms belong to the behavioral-cognitive-physical-psychosocial-
(BCPS)-worsening index consisting of indices of a general cognitive decline (G-CoDe), symptomatome
of schizophrenia, and quality of life (QoL)-phenomenome.

Methods:
FF symptoms were assessed using the Fibromyalgia and Chronic Fatigue Rating scale in 80
schizophrenia patients and 40 healthy controls and serum cytokines/chemokines, IgA levels to TRYCATs,
IgM to OSEs, zonulin and transcellular/paracellular (TRANS/PARA) molecules were assayed
using ELISA methods.

Results:
A large part (42.3%) of the variance in the total FF score was explained by the regression on
the PARA/TRANS ratio, pro-inflammatory cytokines, IgM to zonulin, IgA to TRYCATs (all positively),
and IgM to OSEs (inversely). There were highly significant correlations between the total FF
score and G-CoDe, symtopmatome, QoL phenomenome, and BCPS-worsening score. FF symptoms
belong to a common core shared by G-CoDe, symtopmatome, and QoL phenomenome.

Conclusion:
The physio-somatic symptoms of schizophrenia are driven by various pathways, including
increased zonulin, breakdown of the paracellular tight-junctions pathway, immune activation with
induction of the TRYCAT pathway, and consequent neurotoxicity. It is concluded that FF symptoms
are part of the phenome of schizophrenia and BCPS-worsening as well.
A Novel Hospital-to-Home System for Children With Medical Complexities: Usability Testing Study
Authors: Bird Marissa , Carter Nancy , Lim Audrey , Kazmie Nadia , Fajardo Cindy , Reaume Shannon , McGillion Michael H 
Published: August 2022

Background
Children with medical complexity (CMC) are a group of young people who have severe complex chronic conditions, substantial family-identified service needs, functional limitations, and high health care resource use. Technology-enabled hospital-to-home interventions designed to deliver comprehensive care in the home setting are needed to ease CMC family stress, provide proactive and comprehensive care to this fragile population, and avoid hospital admissions, where possible.

Objective
In this usability testing study, we aimed to assess areas of strength and opportunity within the DigiComp Kids system, a hospital-to-home intervention for CMC and their families and care providers.

Methods
Hospital-based clinicians, family members of medically complex children, and home-based clinicians participated in DigiComp Kids usability testing. Participants were recorded and tasked to think aloud while completing usability testing tasks. Participants were scored on the metrics of effectiveness, efficiency, and satisfaction, and the total usability score was calculated using the Single Usability Metric. Participants also provided insights into user experiences during the postusability testing interviews.

Results
A total of 15 participants (5 hospital-based clinicians, 6 family members, and 4 home-based clinicians) participated in DigiComp Kids usability testing. The participants were able to complete all assigned tasks independently. Error-free rates for tasks ranged from 58% to 100%; the average satisfaction rating across groups was ≥80%, as measured by the Single Ease Question. Task times of participants were variable compared with the task times of an expert DigiComp Kids user. Single Usability Metric scores ranged from 80.5% to 89.5%. In qualitative interviews, participants stressed the need to find the right fit between user needs and the effort required to use the system. Interviews also revealed that the value of the DigiComp Kids system was in its ability to create a digital bridge between hospital and home, enabling participants to foster and maintain connections across boundaries.

Conclusions
Usability testing revealed strong scores across the groups. Insights gained include the importance of tailoring the implementation of the system to match individual user needs, streamlining key system features, and consideration of the meaning attached to system use by participants to allow for insight into system adoption and sustainment.
Pengembangan Metode Ekstraksi Alginat dari Rumput Laut Sargassum sp. sebagai Bahan Pengental
Authors: Husni Amir , Subaryono Subaryono , Pranoto Yudi , Taswir Taswir , Ustadi Ustadi 
Published: August 2022

Indonesia has a lot of seaweed that have high potential as a producer of alginate, but the method of extraction has not been as expected. The objective of this study to develop a method of extraction of sodium alginate from seaweed Sargassum through the calcium alginate pathway. This study used different variations of the concentration of calcium chloride. The concentration of calcium chloride used varied 0.5, 0.75 and 1 M. As a control, the extraction of alginate performed through alginic acid pathway which was developed at the Center for Research Product Processing and Biotechnology of Marine and Fisheries, Jakarta. Quality parameters were observed including alginate yield, product appearance, viscosity and gel strength. The result showed that the yield of alginate produced successively for 32.67; 44.67 and 53.33 % and 31.67 % for controls. In appearance, the concentration of calcium chloride did not significantly affect the alginate product appearance, but darker when compared with the product extracted through the alginic acid. Viscosity alginate produced successively 149, 131 and 144 cP, while 304 cP for control. In general, the alginate gel strength extracted through of calcium alginate pathway is lower than the alginic acid pathway.ABSTRAKIndonesia mempunyai banyak rumput laut yang berpotensi tinggi sebagai penghasil alginate, namun metode ekstraksinya belum sesuai yang diharapkan. Penelitian ini bertujuan untuk mengembangkan metode ekstraksi natrium alginat dari rumput laut Sargassum melalui jalur kalsium alginat. Dalam penelitian ini digunakan berbagai variasi konsentrasi kalsium klorida yang digunakan pada pemisahan alginat dari filtrat hasil ekstraksinya. Konsentrasi kalsium klorida yang digunakan divariasi 0,5; 0,75 dan 1 M. Sebagai kontrol dilakukan ekstraksi alginat melalui jalur asam alginat yang dikembangkan di Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Jakarta. Parameter kualitas alginat yang diamati meliputi rendemen alginat, kenampakan produk, viskositas dan kekuatan gel yang dihasilkan. Hasil pengamatan menunjukkan bahwa rendemen alginat yang dihasilkan berturut-turut sebesar 32,67; 44,67 dan 53,33 % dan untuk kontrol 31,67 %. Secara kenampakan, konsentrasi kalsium klorida tidak terlalu mempengaruhi kenampakan produk alginat yang dihasilkan, tetapi lebih gelap jika dibandingkan dengan produk hasil ekstraksi melalui jalur asam alginat. Viskositas alginat yang dihasilkan berturut-turut 149, 131 dan 144 cP, sementara untuk kontrol 304 cP. Secara umum kekuatan gel alginat yang dihasilkan dari jalur kalsium alginat lebih rendah dibandingkan dengan yang diekstrak melalui jalur asam alginat.
Perbaikan Sifat Mekanik dan Laju Transmisi Uap Air Edible Film dari Pati Ganyong Termodifikasi dengan Menggunakan Lilin Lebah dan Surfaktan
Authors: Santoso Budi , Pratam Filli , Hamzah Basuni , Pambayun Rindit 
Published: August 2022

Edible film from ganyong starch without and with modification were incorporated by CMC and lecithin as surfactants. Edible film were characterized with respect to water vapor transmission rate and mechanical properties. Incorporation of CMC 2 % and lecithin 1 % as surfactants decreased water vapor transmission rate. Puncture strength decreased but still fulfill Japanese Industrial Standard (JIS) 1975 min 50 gf. Elongation of edible film increased and not fulfill JIS 1975 min 70 %.ABSTRAKEdible film pati ganyong sebelum dan setelah dimodifikasi ditambahkan surfaktan CMC dan lesitin. Karakteristik edible film yang diamati adalah laju transmisi uap air dan sifat mekanik (kuat tekan dan persen pemanjangan). Penambahan CMC dengan konsentrasi 2 % dan lesitin 1 % menurunkan laju transmisi uap air edible film pati ganyong. Kuat tekan edible film pati ganyong mengalami penurunan, namun masih memenuhi standar JIS 1975 minimal 50gf. Nilai persen pemanjangan edible film pati ganyong meningkat tetapi belum memenuhi standar JIS 1975.
Actigraphy to Evaluate Changes in Physical Activity After Autologous Breast Reconstruction- A Prospective Comparison Study
Authors: PRUSTY KRISHNA PRASAD 
Published: August 2022

Background-Early ambulation and return to preoperative (baseline) ambulatory level is protective against postoperative venous thromboembolism. The duration of decreased physical activity after surgery is unknown for most procedures, as surgeons typically estimate physical recovery based on subjective patient reporting.
Objectives- To quantify the time it takes to return to baseline ambulatory status after breast reconstruction using actigraphy devices.
Methods- Actigraphy devices were used to evaluate preoperative and postoperative physical activity levels in patients undergoing autologous breast reconstruction at a tertiary care institution. Steps and resting heart rate (HR) were used as metrics of physical activity and physiological state. "Baseline" physical activity was defined by the average daily step count during the 14 days before surgery. "Return to baseline" occurred when the 7-day daily step average was greater than or equal to 95% of their baseline steps. SPSS (version 22.0) was used for analysis.
Results- From May 2020 to April 2021, 30 patients were enrolled in the study before deep inferior epigastric perforator breast reconstruction. The mean age was 49.2 years and mean BMI was 28.6. This cohort averaged 7918 ± 3271 preoperative steps. Two patients returned to baseline activity by postoperative day 28. In total, 8 patients returned by postoperative week 8. Preoperative resting HR average was 73.4 ± 9.33 beats per minute. Actigraphy data demonstrated an initial decrease in activity,increase in sleep variability and increased heart rate that approached the patients’pre-operative normalized data as they recovered over time.

Conclusions:These data demonstrate that actigraphy data would be of interest topatients making breast reconstruction decisions and that the data can be successfullycollected to inform decision-making. These findings indicate that surgeons may underestimate the impact of surgery on physical decline and, consequently, may undertreat with venous thromboembolism prophylaxis.
PENINGKATAN KEMAMPUAN KOGNITIF PESERTA DIDIK MELALUI PEMBELAJARAN STEM BERBASIS LESSON STUDY PADA MATERI PERUBAHAN LINGKUNGAN
Authors: Hasanuddin Hasanuddin , Marlina Marlina , Sukowati Ika 
Published: August 2022

Perkembangan ilmu pengetahuan dan teknologi berubah dan semakin kompetitif. Diperlukan proses pembelajaran yang memfasilitasi bukan menghambat kemampuan kognitif dengan cara melaksanakan proses pembelajaran yang memberi kebebasan peserta didik dalam proses berpikirnya atau dalam proses belajarnya. Penelitian ini bertujuan untuk menganalisis pengaruh pembelajaran STEM berbasis lesson study terhadap kemampuan kognitif peserta didik. Metode yang digunakan dalam penelitian ini adalah kuasi eksperimen . Sampel penelitian terdiri 90 peserta didik dari dua sekolah menengah atas di Kabupaten Aceh Selatan. Parameter yang diukur adalah kemampuan kogntitif dengan menghitung normalisasi gain (n-gain). Instrumen yang digunakan adalah pretes dan postes berbentuk soal pilihan ganda untuk mengukur kemampuan kognitif. Analisis data untuk kemampuan kognitif diuji dengan uji beda dua rata-rata (uji-t). Hasil penelitian menunjukkan n-gain kemampuan kogntif kelas eksperimen sebesar 0,81 termasuk kategori tinggi, sedangkan n-gain kelas kontrol sebesar 0,71. Hasil uji beda rata-rata n-gain antara kedua kelas pada taraf signifikasi 95%, menunjukkan t-hitung 3,46 ˃ t-tabel 1,98 yang berarti terdapat perbedaan signifikan peningkatan kemampuan kognitif pada kelas kontrol dengan kelas eksperimen. Kesimpulan penelitian ini adalah pembelajaran STEM berbasis lesson study meningkatkan kemampuan kognitif secara signifikan
EFEKTIVITAS PENGHAMBATAN BAKTERI KITINOLITIK TERHADAP Fusarium proliferatum
Authors: Azzira Rahma Siti , Rachmania Mubarik Nisa , A Manaf Lisdar 
Published: August 2022

Fusarium spp. merupakan penyebab penyakit pada beberapa jenis tanaman salah satunya bawang merah sehingga dapat menurunkan produktivitas tanaman bawang merah (Allium cepa L.). Bakteri kitinolitik diketahui memiliki sifat antagonis terhadap Fusarium spp. Penelitian bertujuan mengetahui kemampuan isolat bakteri ABP5.1, ABP5.2.2, ABS4.1.2, BBP5.2.2 yang berasal dari pertanian bawang merah dalam menghambat pertumbuhan F. proliferatum. Sebanyak empat isolat bakteri terpilih hasil isolasi dari penelitian sebelumnya diuji kemampuannya dalam menghambat pertumbuhan Fusarium proliferatum. Tahapan penelitian terdiri atas karakterisasi isolat, uji aktivitas kitinolitik, uji antagonis antar isolat bakteri, dan uji antagonis isolat bakteri terhadap cendawan Fusarium proliferatum. Keempat isolat bakteri ABP5.1, ABP5.2.2, ABS4.1.2, dan BBP5.2.1 merupakan bakteri gram negatif. Uji kualitatif kitinolitik menunjukkan keempat isolat menghasilkan zona bening dengan indeks kitinolitik antara 0,04-1,0. Isolat bakteri ABS4.1.2 memiliki nilai indeks kitinolitik terbesar yaitu 1,09. Uji antagonis antar isolat bakteri menunjukkan hasil keempat isolat bakteri tidak bersifat saling antagonis. Uji antagonis bakteri terhadap cendawan Fusarium proliferatum dengan metode dual culture menunjukkan bahwa sebanyak dua dari empat isolat mampu menghambat pertumbuhan cendawan F.proliferatum. Isolat ABP5.2.2 memiliki daya hambat terbesar yaitu 41%, sedangkan isolat ABS4.1.2 memiliki daya hambat sebesar 40%.