bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleic acid direct delivery to fibroblasts: a review of nucleofection and applications
Authors: Ren Ranyue , Guo Jiachao , Liu Guangwu , Kang Hao , Machens Hans-Günther , Schilling Arndt F. , Slobodianski Alex , Zhang Ziyang 
Published: November 2022

AbstractThe fibroblast is one of the ideal target cell candidates for cell-based gene therapy approaches to promote tissue repair. Gene delivery to fibroblasts by viral transfection has been confirmed to have high transfection efficiency. However, in addition to immunogenic effects of viruses, the random integration of viral genes may damage the genome, affect the cell phenotype or even cause cancerous mutations in the transfected cells. Due to these potential biohazards and unknown long-term risks, the clinical use of viral transfection has been very limited. In contrast, initial non-viral transfection methods have been simple and safe to implement, with low immunogenicity, insertional mutagenesis, and risk of carcinogenesis, but their transfection efficiency has been relatively low. Nucleofection, a more recent non-viral transfection method, now combines the advantages of high transfection efficiency and direct nucleic acid delivery to the nucleus with a high safety.Here, we reviewed recent articles on fibroblast nucleofection, summarized different research points, improved methods and application scopes, and opened up ideas for promoting the further improvement and development of fibroblast nucleofection to meet the needs of a variety of disease research and clinical applications.
Maps and stories in the creation of richer accounts of change in pastoral landscapes in Nordland, northern Norway
Authors: Risvoll Camilla , Galafassi Diego , Veland Siri , Pavall Mats , Lifjell Tom , Lundberg Aase Kristine , Eilertsen Svein Morten 
Published: November 2022

AbstractThe categories and concepts in the existing official land-use maps have been under improvements over recent years; however, this study from Nordland, northern Norway, shows that they continue to pose several dilemmas when aiming to better capture the impacts of multiple land uses on reindeer herding. While these developments have done much to better communicate the presence of reindeer herding to developers and planners, there remain significant challenges to achieve best practices. In particular, the confluence of multiple landscape features, for instance, roads, farmland, ecoregions, tenure, pastures, tourism paths and cabins, may have interactions that create cumulative impacts that do not “add up” neatly across map layers. Migration routes, herding routes, and resting areas have been introduced in these maps. In collaboration with reindeer herders, this article analyses how to enrich mapping practices by for example including bottlenecks, parallel to increased attention to influence zones and avoidance zones, as important emergent impacts of multiple interacting features of the landscape. Our research reveals how local knowledge developed by herders through their “presence in the landscape” is better capable of accounting for interactions and cumulative dimensions of landscape features. Through our participatory mapping approach with Sámi reindeer herders, we focus on ways of combining reindeer herders’ knowledge and GIS maps and demonstrate the potential in collaborative work between herders and policymakers in generating a richer understanding of land-use change. We conclude that the practical knowledge of people inhabiting and living with the landscape and its changing character generates a rich understanding of cumulative impacts and can be harnessed for improved land-use mapping and multi-level governance.
Pastoralism in the high Himalayas: Understanding changing practices and their implications for parasite transmission between livestock and wildlife
Authors: Khanyari Munib , Robinson Sarah , Milner-Gulland E. J. , Morgan E. R. , Rana Rashmi Singh , Suryawanshi Kulbhushansingh R. 
Published: November 2022

AbstractRangelands are increasingly being affected by climatic variations, fragmentation and changes in livestock management practices. Along with resource competition between livestock and wildlife, disease transmission has implications for people and wildlife in these shared landscapes. We worked with two pastoral communities in the Western Indian Himalayas: the migratory Kinnauras that travel to the Trans-Himalayan Pin valley in summer and the resident herders of Pin Valley. Asiatic ibex (Capra sibirica) is the predominant wild herbivore in Pin. The pastures in Pin are grazed by both livestock (migratory and resident) and ibex, with the potential for disease transmission. We investigate the effects of herding practices on livestock health and disease transmission, while focusing on gastro-intestinal nematodes (GINs) as they can spread by sharing pasture between wild and domestic ungulates. Surveys were carried out between June and August 2019, the period when migratory Kinnauras, local herders and Asiatic Ibex are found in Pin Valley. We found that the Kinnaura flocks share pasture with ibex during their time in Pin, exhibiting significantly higher endo-parasite burdens than sedentary livestock, and the Kinnaura flocks are increasing in number. This suggests GIN cross-transmission is possible, as GINs have low host specificity and a free-living, environmental stage that is trophically acquired. As local (sedentary) sheep and goats rarely share pasture with ibex, have low endo-parasite burdens and are few in number, they are unlikely to transmit parasites to ibex. However, increasingly large local stock numbers may be contributing to pasture degradation which could cause nutritional stress and resource competition, exacerbating GIN impacts. We also find evidence for transhumance persisting, in spite of signs of pasture degradation that are seemingly affecting livestock productivity and potentially disease transmission. It is critical that proactive measures are taken, like participatory disease management with the Kinnauras, to align livelihoods with wildlife and rangeland conservation.
Follow our path with asparaginase activity: one technique, but different uses in clinical practice
Authors: Cecconello Daiane Keller , Rechenmacher Ciliana , Silva Klerize Anecely de Souza , Scherer Fernanda Fetter , Prates Thomas Dal Bem , Marques Rebeca Ferreira , Daudt Liane Esteves , Michalowski Mariana Bohns 
Published: November 2022

AbstractAcute lymphoblastic leukemia is the most common childhood malignancy. One of the drugs used in the treatment is Asparaginase, and monitoring of its activity levels enables better outcomes. Since 2018, our laboratory has been working to establish a regular analysis of activity. This implementation allowed to qualify care by detecting silent inactivation and also establishing desensitization as a safe way to overcome the lack of Erwinia. We were able to monitor children aged 0 to 18 years who were being treated with PEG-ASNase. The activity was assessed on days 7 (90 samples) and 14 (52 samples) after ASNase infusions. 142 samples were analyzed. 95.7% reached an adequate activity level (≥ 0.1 IU/mL). Patients treated with ASNase can develop allergic reactions. With the activity monitoring, is possible to circumvent situations like these and implement desensitization protocols for patients who had clinical hypersensitivity without inactivation. Desensitization induces temporary unresponsiveness to drug antigens, allowing the patients to proceed with the prescribed chemotherapy. We have received samples from four patients being treated with different desensitization protocols. Patients tolerated the protocols well. Only one had a grade 2 reaction during the infusion and activity < 0.1 IU/mL, which resulted in the switch to Erwinia. The dose adaptation is a possible and more recent use of ASNase monitoring and we were able to confirm the feasibility of PEG-ASNase desensitization protocols.
Aging and distractor resistance in working memory: Does emotional valence matter?
Authors: Ding Lin-jie , Zhang Shao-shuai , Peng Ming , Li Xu 
Published: November 2022

AbstractBackgroundEmotional stimuli used as targets of working memory (WM) tasks can moderate age-related differences in WM performance, showing that aging is associated with reductions in negativity bias. This phenomenon is referred to as the positivity effect. However, there is little research on whether emotional distractors have a similar moderating effect. Moreover, the underlying neural mechanism of this effect has not been studied. In this study, we examined the behavioral and neurophysiological basis for age differences in resistance to emotional distractors within WM.MethodsOlder adults (n = 30, ages 60–74) and young adults (n = 35, ages 19–26) performed a 2-back task in which a digit was superimposed on a face with a happy, angry, or neutral expression as a distractor. Event-related potential (ERP) was simultaneously recorded to assess P2, N2, and later positive potential (LPP) amplitudes.ResultsOlder adults were less accurate and slower than young adults on the WM task. Moreover, the results demonstrated a significant interaction between age and emotional valence on response accuracy, young adults' performance was worse when the distractor was neutral or positive than when it was negative, but there was no effect of the emotional valence of distractors on older adults’ WM performance. ERP analyses revealed greater P2 amplitude in older adults than young adults, regardless of the emotional valence of distractors. However, older adults and young adults did not differ on N2 or LPP amplitude, and negative distractors elicited greater N2 than positive distractors in both age groups.ConclusionsThe behavioral findings provided evidence of age-related reductions in negativity bias. Thus, the behavioral measures indicated a positivity effect in WM. However, the ERP results did not show this same interaction. These discrepant results raise questions about whether and to what extent older and young adults differ in controlling the effect of emotional distractors in WM.
The Persian version of the psychological flexibility in sport scale: a psychometric study
Authors: Badinlou Farzaneh , Badami Rokhsareh , Reinebo Gustaf , Jansson-Fröjmark Markus , Sepehri Fahimeh , Molaviniya Shiva , Lundgren Tobias 
Published: November 2022

Abstract
Background
There is a growing body of research suggesting that psychological flexibility (PF) is an important psychological construct related to psychological health and human performance. The Psychological Flexibility in Sport Scale (PFSS) is the first general scale to assess sport-related PF. So far, the PFSS has not yet been validated in other contexts than Sweden. Therefore, the current study sought to investigate a Persian version of the PFSS (P-PFSS) and extend the investigation of the psychometric properties of the PFSS in Iranian athletes.

Methods
A total of 302 athletes from both team and individual sports (average age of 20.7 years, SD ± 7.5, 62.3% were female) were involved in the current study. Statistical analysis was performed on the data to test validity and reliability. The validity of the P-PFSS was tested through face and content validity, construct validity, criterion validity, and known-groups validity. The reliability of P-PFSS was verified through internal consistency and temporal stability of the scale.

Results
Results revealed that validity of the P-PFSS was satisfactory. The instrument was determined to have strong face and content validity. With modifications, the confirmatory factor analysis confirmed the scale’s unidimensionality. The convergent validity of the P-PFSS was found to be acceptable (average variance extracted = 0.66) and satisfactory results were also found in the correlation matrix for the assessment of construct validity. The P-PFSS showed good criterion validity related to generic psychological flexibility and athletic-related variables. Also, the P-PFSS was able to differentiate PF between known groups. The P-PFSS was found to be reliable, with good internal consistency (Cronbach’s alpha = 0.92; composite reliability = 0.92) and temporal stability on retest (intraclass correlation coefficient = 0.95).

Conclusions
Overall, the Persian version of the PFSS showed good psychometric qualities in Iranian athletes. The current study provides additional support for the PFSS and extends the context-specific utility for practitioners and researchers in assessing sport-related PF.
Bioproduction of testosterone from phytosterol by Mycolicibacterium neoaurum strains: “one-pot”, two modes
Authors: Tekucheva Daria N. , Nikolayeva Vera M. , Karpov Mikhail V. , Timakova Tatiana A. , Shutov Andrey V. , Donova Marina V. 
Published: November 2022

AbstractThe main male hormone, testosterone is obtained from cheap and readily available phytosterol using the strains of Mycolicibacterium neoaurum VKM Ac-1815D, or Ac-1816D. During the first “oxidative” stage, phytosterol (5–10 g/L) was aerobically converted by Ac-1815D, or Ac-1816D to form 17-ketoandrostanes: androstenedione, or androstadienedione, respectively. At the same bioreactor, the 17-ketoandrostanes were further transformed to testosterone due to the presence of 17β-hydroxysteroid dehydrogenase activity in the strains (“reductive” mode). The conditions favorable for “oxidative” and “reductive” stages have been revealed to increase the final testosterone yield. Glucose supplement and microaerophilic conditions during the “reductive” mode ensured increased testosterone production by mycolicibacteria cells. Both strains effectively produced testosterone from phytosterol, but highest ever reported testosterone yield was achieved using M. neoaurum VKM Ac-1815D: 4.59 g/l testosterone was reached from 10 g/l phytosterol thus corresponding to the molar yield of over 66%. The results contribute to the knowledge on phytosterol bioconversion by mycolicibacteria, and are of significance for one-pot testosterone bioproduction from phytosterol bypassing the intermediate isolation of the 17-ketoandrostanes.
Graphical Abstract
A case of long-term survival treated with three metastasectomies and two subsequent adjuvant nivolumab therapies for recurrent malignant melanoma of the esophagus
Authors: Hanada Keita , Tsunoda Shigeru , Nomura Motoo , Fujimura Shintaro , Yutaka Yojiro , Nishigori Tatsuto , Hisamori Shigeo , Maekewa Hisatsugu , Hoshino Nobuaki , Itami Atsushi , Tanaka Eiji , Obama Kazutaka 
Published: November 2022

Abstract
Background
The treatment strategy for metastatic lesions of primary malignant melanoma of the esophagus (PMME) is currently determined on a case-by-case basis, based on the National Comprehensive Cancer Network (NCCN) guidelines for cutaneous melanoma. The NCCN guidelines state that resection should be considered in patients with resectable metastatic recurrence. Herein, we report a case of long-term survival treated with three metastasectomies and two subsequent adjuvant nivolumab therapies for the metastatic recurrence of PMME.

Case presentation
A 65-year-old female patient with PMME underwent thoracoscopic subtotal esophagectomy, gastric tube reconstruction via the posterior mediastinal route, and cervical esophagogastric anastomosis. Histopathological examination of the resected specimen revealed that the tumor was PMME with tumor invasion into the muscularis propria and no lymph node metastasis. At the age of 68 years, she developed intestinal invagination due to jejunal metastasis of malignant melanoma and underwent resection of the jejunum. Histopathological examination of the resected specimen revealed two metastases of malignant melanoma in the jejunum and one metastasis to the mesenteric lymph node. At the age of 75 years, a recurrence of malignant melanoma was found in the cervical esophagus. She underwent thoracoscopic mobilization of the gastric tube and esophagus followed by cervical esophagectomy and reconstruction with a free jejunum flap. She received 24 courses of nivolumab therapy for 1 year as a postoperative adjuvant therapy. Subsequently, at the age of 78 years, an enlarged left cervical lymph node and a mass in the right lower lobe of the lung were found. She underwent left cervical lymph node dissection and thoracoscopic wedge resection of the right lung. Histopathological examination of the resected specimens revealed that both tumors were metastases of malignant melanoma. At age 79 years, she received eight courses of nivolumab therapy as a second postoperative adjuvant therapy, with no sign of recurrence in a 9-month follow-up period after the third metastasectomy.

Conclusion
In cases of metastatic recurrence of PMME, aggressive resection of oligometastasis with postoperative adjuvant nivolumab therapy may result in long-term survival.
Efficacy of neomycin dosing regimens for treating enterotoxigenic Escherichia coli-related post-weaning diarrhoea in a Danish nursery pig herd not using medicinal zinc oxide
Authors: Morsing Malene Kjelin , Larsen Inge , Pedersen Ken Steen , Weber Nicolai Rosager , Nielsen Jens Peter 
Published: November 2022

AbstractNeomycin is a concentration-dependant aminoglycoside antimicrobial used to treat enterotoxigenic Escherichiacoli(ETEC)-related post-weaning diarrhoea (PWD) in pigs. The objective was to compare the efficacy of neomycin administered in a single high dose (50,000 IU/kg) and a standard dose and frequency (25,000 IU/kg daily for 3 consecutive days) in reducing the number of pigs with clinical PWD. We also aimed to evaluate the development of antimicrobial resistance inE. colifollowing neomycin treatment. The study was performed in a Danish herd not using medicinal zinc oxide and experiencing outbreaks of PWD caused by ETEC in the first week after weaning. Pigs from six batches with perianal faecal staining on days 4–6 after weaning and a faecal score of 3–4 were ear tagged and treated with neomycin. Pens were randomly assigned to a treatment group before inclusion. A total of 772 pigs (471 in the control group and 301 in the experimental group) were included and treated orally. The apparent prevalence of diarrhoea on the first day of inclusion across six batches (n = 1,875) was 27%. The efficacy of the neomycin treatment strategy was 86% for the control group and 91% for the single high-dose group (p = 0.043), and the mean percentage (standard deviation (sd)) of haemolyticE. coli-like colonies was 12% (26) and 26% (37) (p < 0.001), respectively. Neomycin resistance did not differ between groups. Before treatment, all analysed isolates were identified as ETEC (n = 142), while after treatment, 91% were identified as ETEC (n = 69) and 9% (n = 7) as non-ETECE. coli(without fimbria or toxins). A higher cure rate in the single high-dose group suggests that ETEC-related PWD can be treated with a single high dose of 50,000 IU/kg of neomycin, thereby reducing antimicrobial use by 33% compared to the standard treatment of 25,000 IU/kg for 3 consecutive days. The study indicated a higher number of haemolyticE. coliin the single high-dose group after treatment, but no evidence of increased neomycin resistance in coliforms was observed compared to the standard treatment.
Knee osteoarthritis pre-screening questionnaire (KOPS): cross-cultural adaptation and measurement properties of the brazilian version—KOPS Brazilian version
Authors: Trombini-Souza Francis , Macedo Ingrid , Yázigi Flávia , Veiga Diogo , Carnide Filomena , Espanha Margarida , Oliveira George , Goldenstein-Schainberg Claudia , Fuller Ricardo 
Published: November 2022

Abstract
Background
Despite the criteria already established for the classification of knee osteoarthritis (OA), a radiographic and/or clinical knee OA diagnosis usually occurs in cases of fully manifest or more advanced disease, which can make health promotion, prevention, and functional rehabilitation in more advanced stages of the disease less effective. In addition, radiographic knee OA can generate more financial costs for health services. Therefore, developing and validating screening instruments to assess the probability of development and progression of knee OA would be of great value for both clinical practice and science.

Objective
To cross-culturally adapt and investigate the measurement properties of the Knee OA Pre-screening Questionnaire Brazilian version.

Methods
A total of 250 individuals of both sexes aged between 35 and 92 years [(mean (standard deviation): 63 (11) years old; 74.1 (15.1) kg; 1.59 (0.09) m; 29.38 (5.44) kg/m2] participated in this study. The cross-cultural adaptation and analyses of the measurement properties of the KOPS Brazilian version included: (1) assessment of conceptual and item equivalence; (2) assessment of semantic equivalence; (3) assessment of operational equivalence; and (4) assessment of measurement equivalence, reliability, and validity.

Results
Cronbach’s alpha for the internal consistency among the six components of the KOPS Brazilian version was 0.71. The test-retest 72 h apart for each component resulted in a coefficient correlation intraclass ranging from 0.74 to 1.00. The probability of an individual randomly chosen from the population having KL ≥ 1 and KOPS Brazilian version ≥ 21 points was 0.74 (area under the curve of the Receiver Operating Characteristic – AUC of ROC); furthermore, the AUC for KL ≥ 2 and the KOPS Brazilian version ≥ 23 points was 0.77.

Conclusion
The KOPS Brazilian version is a reliable and valid instrument for early screening of knee OA in individuals aged 35 years and over in the Brazilian context.
Supplementation of Mulberry (Morus indica) and Vernonia (V. amygdalina) leaves as protein source on morphometric measurement, weight change, and carcass characteristics of sheep
Authors: Mengistu Gezahegn , Assefa Getnet 
Published: November 2022

Abstract
Back ground
Protein source supplement such as noug seed cake (NSC) has a greater cost. To overcome the problems associated with the shortage of protein source supplements, there is a need to look for options of protein source feeds preferably cheap, locally available, and easily accessible by farmers. In this regard, browse tree species such as mulberry (Morus indica) and Vernonia amygdalina represents a valuable option to overcome such problem.

Methods
We evaluated the feed intake, morphometric measurements, weight change, and carcass parameters of 25 male sheep, with initially weighed 20.8 ± 1.7 kg, supplemented for 90 days of experimental period. The treatments (diets) were the replacement of the protein in the NSC with iso-nitrogenous at (0, 25, 50, 75, and 100%) of mulberry and Vernonia mixed leaves meal. The sheep were given a basal diet of Rhodes grass hay, supplemented with 400.0 (D1), 429.8 (D2), 459.5 (D3), 489.3 (D4), and 519.0 (D5) g day− 1. The design was randomized complete block design (RCBD) with five animals in each treatment and replication.

Results
The total DM intake (g day− 1) was significantly higher in D3 (771.4) than in D1 (722.8) and D5 (642.8) but similar to D2 (754.9) and D4 (759.7). Chest-depth and hip-width were significantly influenced by the diets. The average daily gain of sheep in D1 (87.7), D2 (82.0), D3 (83.4), and D4 (75.2) were significantly higher than in D5 (56.0 g day− 1). The hot carcass weight of sheep fed D1 (14.1), D2 (13.7), D3 (12.7), and D4 (12.8) were significantly higher than sheep fed D5 (10.9 kg). Likewise, dressing percentage of sheep for D1 to D4 were higher than D5.

Conclusion
The results of the present investigation confirm that replacement of noug seed cake with mulberry and Vernonia mixed leaves meal up to 75% (25% NSC + 75% mulberry and Vernonia mixed leaves) achieved comparable result with concentrate mix in weight gain and carcass yield.