bluuebunny/crossref_metadata_2025_split huggingface dataset:
Goal-directed vocal planning in a songbird
Authors: Zai Anja T. , Stepien Anna E. , Giret Nicolas , Hahnloser Richard H.R. 
Published: September 2022

AbstractSongbirds’ vocal mastery is impressive, but to what extent is it a result of practice? Can they, based on experienced mismatch with a known target, plan the necessary changes to recover the target in a practice-free manner without intermittently singing? In adult zebra finches, we drive the pitch of a song syllable away from its stable (baseline) variant acquired from a tutor, then we withdraw reinforcement and subsequently deprive them of singing experience by muting or deafening. In this deprived state, birds do not recover their baseline song. However, they revert their songs towards the target by about one standard deviation of their recent practice, provided the sensory feedback during the latter signaled a pitch mismatch with the target. Thus, targeted vocal plasticity does not require immediate sensory experience, showing that zebra finches are capable of goal-directed vocal planning.
Cones and Cone Pathways Remain Functional in Advanced Retinal Degeneration
Authors: Ellis Erika M. , Paniagua Antonio E. , Scalabrino Miranda L. , Thapa Mishek , Rathinavelu Jay , Jiao Yuekan , Williams David S. , Field Greg D. , Fain Gordon L. , Sampath Alapakkam P. 
Published: September 2022

ABSTRACTMost defects causing retinal degeneration in retinitis pigmentosa (RP) are rod-specific mutations, but the subsequent degeneration of cones, which produces loss of daylight vision and high-acuity perception, is the most debilitating feature of the disease. To understand better why cones degenerate and how cone vision might be restored, we have made the first single-cell recordings of light responses from degenerating cones and retinal interneurons after most rods have died and cones have lost their outer-segment disk membranes and synaptic pedicles. We show that degenerating cones have functional cyclic-nucleotide-gated channels and can continue to give light responses, apparently produced by opsin localized either to small areas of organized membrane near the ciliary axoneme or distributed throughout the inner segment. Light responses of second-order horizontal and bipolar cells are smaller and less sensitive but surprisingly similar to those of normal retina. Furthermore, retinal output as reflected in responses of ganglion cells is largely intact at cone-mediated light levels. Together, these findings show that cones and their retinal pathways can remain functional even as degeneration is progressing, an encouraging result for future research aimed at enhancing the light sensitivity of residual cones to restore vision in patients with genetically inherited retinal degeneration.
Distinct Activation Mechanisms Regulate Subtype Selectivity of Cannabinoid Receptors
Authors: Dutta Soumajit , Shukla Diwakar 
Published: September 2022

AbstractCannabinoid receptors (CB1 and CB2) are important drug targets for inflammation, obesity, and other central nervous system disorders. However, due to sequence and structural similarities of the ligand binding pockets of these receptors, most of the ligands lack subtype selectivity and cause off-target side effects. CB2 selective agonists can potentially treat pain and inflammation without the psychoactive effects of CB1 agonism. We hypothesize that the subtype selectivity of designed selective ligands can be explained by ligand binding to the conformationally distinct states between CB1 and CB2. To find these conformationally distinct states, we perform ∼ 700μs of unbiased simulations to study the activation mechanism of both the receptors in absence of ligands. The simulation datasets of two receptors were analyzed using Markov state models to identify similarities and distinctions of the major conformational changes associated with activation and allosteric communication between them. Specifically, toggle switch residue movement and its effect on receptor activation differ greatly between CB1 and CB2. Upon further analysis, we discretize the conformational ensembles of both receptors into metastable states using the neural network-based VAMPnets. Structural and dynamic comparisons of these metastable states allow us to decipher a coarse-grained view of protein activation by revealing sequential conversion between these states. Specifically, we observe the difference in the binding pocket volume of different metastable states of CB1, whereas there are minimal changes observed in the CB2. Docking analysis reveals that differential binding pocket volume leads to distinct binding poses and docking affinities of CB2 selective agonists in CB1. Only a few of the intermediate metastable states of CB1 shows high affinity towards CB2 selective agonists. On the other hand, all the CB2 metastable states show a similar affinity for CB2 selective agonists, explaining these ligands’ overall higher affinity towards CB2. Overall, this computational study mechanistically explains the subtype selectivity of CB2 selective ligands by deciphering the activation mechanism of cannabinoid receptors.
The phylogenetic placement of an enigmatic moth Egybolis vaillantina based on museomics
Authors: Zahiri Reza , Holloway Jeremy D. , Ghanavi Hamid Reza , Aarvik Leif , Wahlberg Niklas 
Published: September 2022

AbstractHere, we present multi-locus sequencing results from the enigmatic Afrotropical monotypic genus Egybolis Boisduval (occurring in East- and South Africa – previously placed in the subfamily Catocalinae, Noctuidae). Model-based phylogenetic analysis places Egybolis within a strongly supported clade comprising four Old World Tropical genera Cocytia Boisduval, Avatha Walker, Anereuthina Hübner and Serrodes Guenée from the family Erebidae, subfamily Erebinae. Hence, we propose to formally assign the monotypic genus Egybolis to the subfamily Erebinae, and the tribe Cocytiini. Timing of divergence analysis reveals the late Oligocene origin around 25 million years ago (mya) for the tribe Cocytiini, an early Miocene i.e., about 21 mya for the split between Cocytia and Egybolis.
Spatial control of ARGONAUTE-mediated RNA silencing in anther development
Authors: Tamotsu Hinako , Koizumi Koji , Briones Alejandro Villar , Komiya Reina 
Published: September 2022

AbstractArgonaute protein (AGO) in association with small RNAs is the core machinery of RNA silencing, an essential mechanism for precise development and defense against pathogens in many organisms. Here, we identified two AGOs in rice anthers, AGO1b and AGO1d, that interact with phased small interfering RNAs (phasiRNAs) derived from numerous long non-coding RNAs. Moreover, 3D-immunoimaging and mutant analysis demonstrated that rice AGO1b and AGO1d redundantly and cell type-specifically regulate anther development by acting as mobile carriers of these phasiRNAs from the somatic cell layers to the germ cells in anthers. Our study also highlights a new mode of reproductive RNA silencing via the specific nuclear and cytoplasmic localization of three AGOs, AGO1b, AGO1d, and MEL1, in rice pollen mother cells.
FOXP1 orchestrates neurogenesis in human cortical basal radial glial cells
Authors: Park Seon Hye E. , Kulkarni Ashwinikumar , Konopka Genevieve 
Published: September 2022

SummaryDuring cortical development, human basal radial glial cells (bRGCs) are highly capable of sustained self-renewal and neurogenesis. Selective pressures on this cell type may have contributed to the evolution of the human neocortex, leading to an increase in cortical size. bRGCs have enriched expression for Forkhead Box P1 (FOXP1), a transcription factor implicated in neurodevelopmental disorders such as autism spectrum disorder. However, the cell type-specific roles of FOXP1 in bRGCs during cortical development remain unexplored. Here, we examine the requirement for FOXP1 gene expression regulation underlying the production of bRGCs using human brain organoids. We examine a developmental time point when FOXP1 expression is highest in the cortical progenitors, and the bRGCs, in particular, begin to actively produce neurons. With the loss of FOXP1, we show a reduction in the number of bRGCs, as well as reduced proliferation and differentiation of the remaining bRGCs, all of which lead to reduced numbers of excitatory cortical neurons over time. Using single-nuclei RNA sequencing and cell trajectory analysis, we uncover a role for FOXP1 in directing cortical progenitor proliferation and differentiation by regulating key signaling pathways related to neurogenesis and neurodevelopmental disorders. Together, these results demonstrate that FOXP1 regulates human-specific features in early cortical development.
Quartet DNA reference materials and datasets for comprehensively evaluating germline variants calling performance
Authors: Ren Luyao , Duan Xiaoke , Dong Lianghua , Zhang Rui , Yang Jingcheng , Gao Yuechen , Peng Rongxue , Hou Wanwan , Liu Yaqing , Li Jingjing , Yu Ying , Zhang Naixin , Shang Jun , Liang Fan , Wang Depeng , Chen Hui , Sun Lele , Hao Lingtong ,   The Quartet Project Team, Scherer Andreas , Nordlund Jessica , Xiao Wenming , Xu Joshua , Tong Weida , Hu Xin , Jia Peng , Ye Kai , Li Jinming , Jin Li , Shi Leming , Hong Huixiao , Wang Jing , Fan Shaohua , Fang Xiang , Zheng Yuanting 
Published: September 2022

AbstractCurrent methods for evaluating the accuracy of germline variant calls are restricted to easy-to-detect high-confidence regions, thus ignoring a substantial portion of difficult variants beyond the benchmark regions. We established four DNA reference materials from immortalized cell lines derived from a Chinese Quartet including parents and monozygotic twins. We integrated benchmark calls of 4.2 million small variants and 15,000 structural variants from multiple platforms and bioinformatic pipelines for evaluating the reliability of germline variant calls inside the benchmark regions. The genetic built-in-truth of the Quartet family design not only improved sensitivity of benchmark calls by removing additional false positive variants with apparently high quality, but also enabled estimation of the precision of variants calls outside the benchmark regions. Batch effects of variant calling in large-scale DNA sequencing efforts can be effectively identified with the concurrent use of the Quartet DNA reference materials along with study samples, and can be alleviated by training a machine learning model with the Quartet reference datasets to remove potential artifact calls. Matched RNA and protein reference materials were also established in the Quartet project, thereby enabling benchmark calls constructed from DNA reference materials for evaluation of variants calling performance on RNA and protein data. The Quartet DNA reference materials from this study are a resource for objective and comprehensive assessment of the accuracy of germline variant calls throughout the whole-genome regions.
Conflict with customers: the limits of social support and job autonomy in preventing burnout among customer service workers
Authors: Klingbyle Alyssa T. , Chung-Yan Greg A. 
Published: September 2022

Purpose
The purpose of this study is to examine the burnout of workers in customer service roles as a result of conflict with customers; and the role that coworker support, non-work-related social support and job autonomy play in buffering customer service workers from conflict with customers.

Design/methodology/approach
A sample of 191 young customer service workers completed an online self-report questionnaire.

Findings
Although it was found that coworker support, non-work-related social support and job autonomy moderated the relationship between customer conflict and burnout, the form of the interactions was not as expected. Rather than buffering customer service workers specifically against customer conflict, it was found that as customer conflict intensifies, it gradually erodes the positive benefits that coworker support, general social support and job autonomy have in preventing burnout as a result of general work stress.

Originality/value
This study is one of few to empirically investigate the unique stressors experienced by customer service workers. It also expands understanding of social support and job autonomy in the context of work stress, demonstrating that there are limits to the effectiveness of these personal and organizational resources in preserving worker well-being.
Market amplification or transformation? The role of industry analysts in spreading WOM in B2B
Authors: Cowan Kirsten , Palo Teea , Chapple Duncan , Zhang Yiwei 
Published: September 2022

Purpose
The purpose of this study is to address these questions. Word-of-mouth (WOM) is increasingly important in business-to-business (B2B) decision-making. Yet, research on this topic is rather limited, and often borrows from business-to-consumer (B2C) WOM literature. The question remains as to whether these assumptions realistically occur in B2B WOM. Specifically, this study explores the following questions: What value does B2B WOM have? Why do social media influencers in B2B engage in WOM? What type(s) of social media influencers spread WOM in B2B?

Design/methodology/approach
To address these questions, this study adopts a qualitative research strategy. This study focuses on industry analysts in information technology markets who often influence the buying decisions of customers through their expertise and recommendations of technology solutions. Based on interviews with these influencers, this study explicates B2B WOM, an area the authors know much less of in comparison to B2C WOM.

Findings
This study reveals differences in who spreads WOM within B2B, their roles, key features of their content and how they spread WOM. Second, this study demonstrates the types of actors spreading WOM in B2B in relation to the type of WOM and how it actually influences B2B markets.

Originality/value
This study broadens the current definition of WOM and, specifically, showcases WOM not only as amplifying messages but as a means to co-create the market itself with vendors and clients. This research offers several contributions to the B2B WOM literature and influencer practice.
The origins and informed uses of the terms phenomenography and phenomenology
Authors: Cibangu Sylvain K. 
Published: September 2022

PurposeThe purpose of this short reflection is to allow for an informed use of both phenomenography and phenomenology in information studies and cognate fields.Design/methodology/approachThe paper apprises uses of phenomenography found particularly in accounts of information literacy commonly describing phenomenography as distinct from phenomenology.FindingsBoth phenomenography and phenomenology continue to hold much credence in methods applied across scores of academic fields, with information studies being among those in the vanguard. Claims displaying differences of phenomenography from phenomenology are misleading and incomplete descriptions of phenomenology.Originality/valueThe paper presents newer materials on the origins of phenomenography and phenomenology to advocate for tighter relationships between and clearer applications of these methods in information studies and beyond.
An investigation of barriers to the application of building information modelling in Nigeria
Authors: Toyin James Olaonipekun , Mewomo Modupe Cecilia 
Published: September 2022

Purpose
The utilisation of building information modelling (BIM) technology is rapidly increasing among construction professionals across the world. Notwithstanding, recent studies revealed a low level of BIM implementation in the context of the Nigerian construction sector. Moreover, previous studies have established that BIM application comes with its share of various barriers. Therefore, this study aims to carry out an on-site survey on barriers to the application of BIM on construction sites in the Nigerian construction industry.

Design/methodology/approach
An extensive review of literature on BIM barriers was conducted, from where 33 factors were identified as significant BIM barriers peculiar to the developing countries. A questionnaire was developed and distributed to the targeted respondents, who are practicing professionals in the Nigerian construction industry, based on the identified barriers. The data collected were analysed by using both descriptive and inferential statistics.

Findings
The principal component analysis revealed that 27 barriers were peculiar to the Nigerian construction industry. The “lack of familiarity with BIM capacity, habitual resistance to change from the traditional style of design and build, and poor awareness of BIM benefit” were identified as the three most critical barriers hindering BIM application on construction sites in the Nigerian construction industry.

Practical implications
This study reveals key information on the peculiar barriers to BIM application in the Nigerian construction industry. The avoidance of these barriers will not only assist various construction stakeholders in the successful implementation of BIM application on a construction project but also promote information management systems and productivity within the construction industry to a great extent. These will further improve post-construction activities.

Originality/value
This study provides a substantial understanding of BIM state of the art in the context of barriers hindering BIM application on construction sites in the Nigerian construction industry.
“Cardboard gangsters”, “in crowd” and “no control”: a case study of autism spectrum disorder in the prison environment
Authors: Allely Clare Sarah , Wood Toni 
Published: September 2022

Purpose
The purpose of this paper is to add to the relatively limited existing literature exploring the experience of individuals with autism spectrum disorder in the prison environment. Prisoners with autism spectrum disorder (ASD) need to be identified in order that they are given the necessary and appropriate support and safeguards. It is increasingly recognised that, for individuals with ASD, prison can be more challenging. This can be due to ASD traits which can make problems occur in everyday life. Some of these ASD traits include: obsessions, compulsions and difficulties in communicating with others. There is increasing recognition that adults with ASD who are in prison are more vulnerable to bullying, social isolation, sexual victimisation and exploitation confrontations with other prisoners. Given this, more research is warranted in this area highlighting the specific needs and challenges of individuals with ASD in the prison.

Design/methodology/approach
This paper is a case study of Mr C.T., a British Citizen, who is currently serving a life sentence (discretionary). Mr C.T. has spent more than 10 years in prison. At the time of completing the questionnaire for the present study, Mr C.T. was 51 years of age. He pled guilty to charges of lewd, indecent and libidinous practices and behaviour; making an indecent photograph contrary to s52(1)(a) of the Civic Government (Scotland) Act 1982; and possession of indecent photographs contrary to s52(A)(1) of the Civic Government (Scotland) Act 1982. Mr C.T. was convicted of Lewd, Indecent and Libidinous Practices and Behaviour and 2 x Civic Government (Scotland) Act, Section 52 (1) (a) and was sentenced to Life Imprisonment with a punishment part of 6 years.

Findings
The case study discussed in this paper clearly raises a number of issues and concerns that urgently need attention in our criminal justice systems.

Originality/value
There is relatively little research exploring the experiences and challenges faced by individuals with ASD. This paper will add to the existing body of research in this limited field.
Can micro-enterprises use Tawarruq Fardi Financing (TFF) for fund raising?
Authors: Siddiky Sumaira , Swandaru Randi , Muneeza Aishath 
Published: September 2022

PurposeMicro-enterprises, like any other business entity, face financing challenges. However, micro-enterprises often cannot access financial institutions as they cannot fulfill the conditions to obtain financing facilities from a formal financial institution. As such, they have to rely on family or friends for financing needs. The most critical challenge faced in this regard to Muslim micro-enterprises is finding out a way in which family and friends could give a financial helping hand without the involvement of riba (interest). At the same time, the person giving the financing can enjoy a profit. This paper aims to propose the Tawarruq Fardi Financing (TFF) model that Islamic micro-enterprises could use to fulfill their financial needs. It becomes a solution when obtaining financing from friends and family or any other third party who could be a private investor who does not want to engage in an equity relationship.Design/methodology/approachThis study adopts a qualitative research methodology, combining descriptive and content analysis using the inductive reasoning approach.FindingsThe paper's outcome shows that the proposed TFF could assist Islamic micro-enterprises in obtaining Shariah-compliant financing without engaging in an equity partnership. It allows them to fulfill their financing needs bearing in mind the interest of both parties involved in the transaction.Originality/valueThis research will assist Islamic micro-enterprises to find out a Shariah-compliant financing facility from family, friends and any other private investors without entering into an equity relationship. The proposed model would be a Shariah-compliant alternative to interest-free loans and engaging in an equity relationship for the sake of getting benefits for both parties involved in the transaction.
The Nordic formula: an analysis of policy learning in sectorial networks of the Nordic Council of Ministers
Authors: Nedergaard Peter , Nobel Trine C. 
Published: September 2022

AbstractOrganised policy learning among the Nordic countries—Finland, Norway, Sweden, Denmark and Iceland—has been around for more than 50 years, but it is an under‐researched subject. This article analyses the process as well as the output of policy learning among Nordic countries on adult education in networks under the auspices of the Nordic Council of Ministers. The data material consists of 10 in‐depth interviews with actors herein over a period of 2 years, as well as the few documents available. The most important results are that the policy learning process is characterised by so‐called epistemic and reflexive learning modes dominated by cooperation, inputs from science and dialogue. The output from the Nordic policy learning networks mainly consists of combining elements from other Nordic countries that are frontrunners in the relevant policy area. The output of Nordic policy learning is certainly much more than just being inspired by practices in Nordic countries. The results from the analysed networks can easily be generalised to other Nordic networks, but to a lesser extent to international policy learning networks where ‘value consensus’ is not as pronounced as among the Nordic countries.