bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exercise oscillatory breathing in heart failure with reduced ejection fraction: clinical implication
Authors: da Luz Goulart Cássia , Agostoni Piergiuseppe , Salvioni Elisabetta , Kaminsky Leonard A , Myers Jonathan , Arena Ross , Borghi-Silva Audrey 
Published: June 2022

Abstract

Aim
The aim of the study is (i) to evaluate the impact of exercise oscillatory ventilation (EOV) in patients with heart failure (HF) with reduced left ventricular ejection fraction (HFrEF) during cardiopulmonary exercise testing (CPET) compared with no EOV (N-EOV); (ii) to identify the influence of EOV persistence (P-EOV) and EOV disappearance (D-EOV) during CPET on the outcomes of mortality and hospitalization in HFrEF patients; and (iii) to identify further predictors of mortality and hospitalization in patients with P-EOV.

Methods and results
Three hundred and fifteen stable HFrEF patients underwent CPET and were followed for 35 months. We identified 202 patients N-EOV and 113 patients with EOV. Patients with EOV presented more symptoms [New York Heart Association (NYHA) III: 35% vs. N-EOV 20%, P &lt; 0.05], worse cardiac function (LVEF: 28 ± 6 vs. N-EOV 39 ± 1, P &lt; 0.05), higher minute ventilation/carbon dioxide production (V̇E/V̇CO2 slope: 41 ± 11 vs. N-EOV 37 ± 8, P &lt; 0.05) and a higher rate of deaths (26% vs. N-EOV 6%, P &lt; 0.05) and hospitalization (29% vs. N-EOV 9%, P &lt; 0.05). Patients with P-EOV had more severe HFrEF (NYHA IV: 23% vs. D-EOV: 9%, P &lt; 0.05), had worse cardiac function (LVEF: 24 ± 5 vs. D-EOV: 34 ± 3, P &lt; 0.05) and had lower peak oxygen consumption (V̇O2) (12.0 ± 3.0 vs. D-EOV: 13.3 ± 3.0 mLO2 kg–1.min–1, P &lt; 0.05). Among P-EOV, other independent predictors of mortality were V̇E/V̇CO2 slope ≥36 and V̇O2 peak ≤12 mLO2 kg–1 min–1; a V̇E/V̇CO2 slope≥34 was a significant predictor of hospitalization. Kaplan–Meier survival analysis showed that HFrEF patients with P-EOV had a higher risk of mortality and higher risk of hospitalization (P &lt; 0.05) than patients with D-EOV and N-EOV.

Conclusion
In HFrEF patients, EOV persistence during exercise had a strong prognostic role. In P-EOV patients, V̇E/V̇CO2 ≥36 and V̇O2 peak ≤12 mLO2 kg–1 min–1 had a further additive negative prognostic role.
Histamine H2 receptor antagonist exposure was related to decreased all-cause mortality in critical ill patients with heart failure: a cohort study
Authors: Huang Yan-Hua , Cai Wen-ke , Yin Sun-Jun , Wang Ping , Li Zhi-Ran , Yang Qin , Zhou Tao , Meng Rui , Yang Mei , Guo Yu , He Gong-Hao 
Published: June 2022

Abstract

Aims
Previous studies reported that histamine H2 receptor antagonists (H2RAs) had cardioprotective effects. However, the effect of H2RAs on mortality of critical ill patients with heart failure (HF) remains unclear. The aim of this study was to clarify the association between H2RAs and all-cause mortality of critical ill patients with HF based on Medical Information Mart for Intensive Care III database (MIMIC-III).

Methods and results
Propensity score matching (PSM) was applied to account for the baseline differences between two groups that were exposed to H2RAs or not. The study primary outcome was all-cause mortality. Kaplan–Meier curves and multivariable Cox regression models were employed to estimate the effects of H2RAs on mortality of critical ill patients with HF. A total of 10 387 patients were included, involving 4440 H2RAs users and 5947 non-H2RAs users. After matching, 3130 pairs of patients were matched between H2RAs users and non-H2RAs users. The results showed significant association between H2RAs exposure and decreased 30-day, 90-day, and 1-year mortality in both univariate analyses and multivariate analyses [hazard ratio (HR) = 0.73, 95% confidence interval (CI): 0.65–0.83 for 30-day; HR = 0.80, 95%CI: 0.72–0.89 for 90-day; and HR = 0.83, 95%CI: 0.76–0.90 for 1-year mortality, respectively] by Cox regression after PSM. Furthermore, stratified analyses revealed that the 30-day, 90-day, and 1-year mortality of ranitidine users were significantly lower than those of famotidine users, respectively.

Conclusion
Histamine H2 receptor antagonists exposure was associated with lower mortality in critical ill patients with HF. Furthermore, ranitidine might be superior to famotidine in reducing mortality of critical ill patients with HF.
Isolation, diversity and antimicrobial activity of planctomycetes from the Tejo river estuary (Portugal)
Authors: Vitorino Inês Rosado , Lobo-da-Cunha Alexandre , Vasconcelos Vítor , Vicente Francisca , Lage Olga Maria 
Published: June 2022

Abstract
The discovery of new bioactive compounds is an invaluable aid to the development of new drugs. Strategies for finding novel molecules can focus on the exploitation of less studied organisms and ecosystems such as planctomycetes and brackish habitats. The unique cell biology of the underexplored Planctomycetota mean it is of particular interest. In this study, we aimed to isolate planctomycetes from the estuary of the Tejo river (Portugal). To reach this goal, macroalgae, water and sediments were sampled and diverse media and isolation techniques applied. Sixty-nine planctomycetal strains were brought into pure culture. An analysis of the 16S rRNA genes found that the majority of the isolates were affiliated to the genus Rhodopirellula. Putative novel taxa belonging to genera Stieleria and Rhodopirellula were also isolated and characterized morphologically. Enterobacterial repetitive intergenic consensus fingerprinting analyses showed higher diversity and different genotypes within close strains. Relevant biosynthetic gene clusters were found in most isolates and acetone extracts from representative strains exhibited mild antimicrobial activities against Escherichia coli and Staphylococcus aureus. Our work has not only enlarged the number and diversity of cultured planctomycetes but has also shown the potential for the discovery of bioactive compounds from the novel taxa.
The MyLO CRISPR-Cas9 toolkit: a markerless yeast localization and overexpression CRISPR-Cas9 toolkit
Authors: Bean Björn D M , Whiteway Malcolm , Martin Vincent J J 
Published: June 2022

Abstract
The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) - CRISPR-associated protein 9 (Cas9)-based genome editing tools, we constructed a Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 toolkit with 3 components: (1) a set of optimized Streptococcus pyogenes Cas9-guide RNA expression vectors with 5 selectable markers and the option to either preclone or cotransform the gRNAs; (2) vectors for the one-step construction of integration cassettes expressing an untagged or green fluorescent protein/red fluorescent protein/hemagglutinin-tagged gene of interest at one of 3 levels, supporting localization and overexpression studies; and (3) integration cassettes containing moderately expressed green fluorescent protein- or red fluorescent protein-tagged compartmental markers for colocalization experiments. These components allow rapid, high-efficiency genomic integrations and modifications with only transient selection for the Cas9 vector, resulting in markerless transformations. To demonstrate the ease of use, we applied our complete set of compartmental markers to colabel all target subcellular compartments with green fluorescent protein and red fluorescent protein. Thus, the MyLO toolkit packages CRISPR-Cas9 technology into a flexible, optimized bundle that allows the stable genomic integration of DNA with the ease of use approaching that of transforming plasmids.
The association between disruption of the circadian rhythm and aggravation of colitis in mice
Authors: Chen Yi-Dong , Zhao Rui-Feng , Zheng Gen , Ling Fang-Mei , Li Jun-Rong , Xu Ming-Yang , Guo Di , Zhang Qiu-Lei , Li Shuang , Zhu Liang-Ru 
Published: June 2022

Abstract
Delayed recovery from ulcerative colitis is mainly due to impaired healing of the intestinal epithelium after inflammation. The circadian rhythm controls cell proliferation and energy metabolism. However, the role of circadian genes in inflammatory bowel disease is largely unknown. The purpose of this study was to investigate whether disrupting the circadian rhythm in mice can worsen colitis by altering mitochondrial energy metabolism. Mice in the experimental groups were under physiologic stress with an 8-h light shift jet-lag schedule every 3 days, whereas those in the control group were not. Subsequently, half of the mice in the control and jet-lagged groups were given dextran sodium sulfate (DSS) to induce colitis. Mice in each group were euthanized at zeitgeber time (ZT)0, ZT4, ZT8, ZT12, ZT16, and ZT20. To investigate the effects of jet lag on the mice, colon specimens were subjected to hematoxylin and eosin staining to analyse mRNA and protein expression of core circadian clock genes (Bmal1, Clock, Per1, Per2, Cry1, Cry2, and Nr1d1). We analysed the mitochondrial morphology, adenosine triphosphate (ATP) levels, and the expression of dynamin-related protein 1 (Drp1) and ser637-phosphorylated (p)-Drp1, which are closely related to ATP production. We further investigated the effect of PER2 knock-down in the colon epithelial cells (CCD 841 CoN) by measuring ATP and cell proliferation levels. Disrupting the circadian rhythm changed the oscillation of clock genes in the colon of mice, altered the mitochondrial morphology of the colon specimens, decreased the expression of p-Drp1, reduced ATP production, and exacerbated inflammatory responses in mice with DSS-induced colitis. Additionally, silencing of PER2 in the colon epithelial cells reduced ATP production and cell proliferation. Disrupting the circadian rhythm in mice decreases mitochondrial energy metabolism in the colon and exacerbates symptoms of colitis.
A 3-D crustal shear wave velocity model and Moho map below the Semail Ophiolite, eastern Arabia
Authors: Weidle C , Wiesenberg L , El-Sharkawy A , Krüger F , Scharf A , Agard P , Meier T 
Published: June 2022

SUMMARYThe Semail Ophiolite in eastern Arabia is the largest and best-exposed slice of oceanic lithosphere on land. Detailed knowledge of the tectonic evolution of the shallow crust, in particular during and after ophiolite obduction in Late Cretaceous times is contrasted by few constraints on physical and compositional properties of the middle and lower continental crust below the obducted units. The role of inherited, pre-obduction crustal architecture remains therefore unaccounted for in our understanding of crustal evolution and the present-day geology. Based on seismological data acquired during a 27-month campaign in northern Oman, Ambient Seismic Noise Tomography and Receiver Function analysis provide for the first time a 3-D radially anisotropic shear wave velocity (VS) model and a consistent Moho map below the iconic Semail Ophiolite. The model highlights deep crustal boundaries that segment the eastern Arabian basement in two distinct units. The previously undescribed Western Jabal Akhdar Zone separates Arabian crust with typical continental properties and a thickness of ∼40–45 km in the northwest from a compositionally different terrane in the southeast that is interpreted as a terrane accreted during the Pan-African orogeny in Neoproterozoic times. East of the Ibra Zone, another deep crustal boundary, crustal thickness decreases to 30–35 km and very high lower crustal VS suggest large-scale mafic intrusions into, and possible underplating of the Arabian continental crust that occurred most likely during Permian breakup of Pangea. Mafic reworking is sharply bounded by the (upper crustal) Semail Gap Fault Zone, northwest of which no such high velocities are found in the crust. Topography of the Oman Mountains is supported by a mild crustal root and Moho depth below the highest topography, the Jabal Akhdar Dome, is ∼42 km. Radial anisotropy is robustly resolved in the upper crust and aids in discriminating dipping allochthonous units from autochthonous sedimentary rocks that are indistinguishable by isotropic VS alone. Lateral thickness variations of the ophiolite highlight the Haylayn Ophiolite Massif on the northern flank of Jabal Akhdar Dome and the Hawasina Window as the deepest reaching unit. Ophiolite thickness is ∼10 km in the southern and northern massifs, and ≤5 km elsewhere.
Carnosol, a diterpene present in rosemary, increases ELP1 levels in familial dysautonomia patient-derived cells and healthy adults: a possible therapy for FD
Authors: Anderson Sylvia L , Fasih-Ahmad Faaria , Evans Anthony J , Rubin Berish Y 
Published: June 2022

Abstract
Recent research on familial dysautonomia (FD) has focused on the development of therapeutics that facilitate the production of the correctly spliced, exon 20-containing, transcript in cells and individuals bearing the splice-altering, FD-causing mutation in the elongator acetyltransferase complex subunit I (ELP1) gene. We report here the ability of carnosol, a diterpene present in plant species of the Lamiaceae family, including rosemary, to enhance the cellular presence of the correctly spliced ELP1 transcript in FD patient-derived fibroblasts by upregulating transcription of the ELP1 gene and correcting the aberrant splicing of the ELP1 transcript. Carnosol treatment also elevates the level of the RNA binding motif protein 24 (RBM24) and RNA binding motif protein 38 (RBM38) proteins, two multifunctional RNA-binding proteins. Transfection-mediated expression of either of these RNA binding motif (RBMs) facilitates the inclusion of exon 20 sequence into the transcript generated from a minigene-bearing ELP1 genomic sequence containing the FD-causing mutation. Suppression of the carnosol-mediated induction of either of these RBMs, using targeting siRNAs, limited the carnosol-mediated inclusion of the ELP1 exon 20 sequence. Carnosol treatment of FD patient peripheral blood mononuclear cells facilitates the inclusion of exon 20 into the ELP1 transcript. The increased levels of the ELP1 and RBM38 transcripts and the alternative splicing of the sirtuin 2 (SIRT2) transcript, a sentinel for exon 20 inclusion in the FD-derived ELP1 transcript, are observed in RNA isolated from whole blood of healthy adults following the ingestion of carnosol-containing rosemary extract. These findings and the excellent safety profile of rosemary together justify an expedited clinical study of the impact of carnosol on the FD patient population.
Substance Use in Relation to Mental Illness: A Study of Mexican-Origin Latinos with Schizophrenia and Their Family Members
Authors: Hernandez Mercedes , Barrio Concepcion , Helu-Brown Paula , Lim Caroline , Gaona Lizbeth 
Published: June 2022

Abstract
Little is known about family context and substance use behaviors among Latinos with schizophrenia. Learning about patient and family caregiver perceptions of use is critical to our understanding of how best to support these vulnerable patients and family caregivers. This study explored perceptions of substance use in relation to mental illness among Latinos primarily of Mexican origin with schizophrenia and their family caregivers. Semistructured interviews were conducted with 34 participants (20 family caregivers; 14 patients) with direct and indirect substance use experience, in their preferred language. Data were analyzed using thematic analysis, which consisted of comparing codes across and within patient and family caregiver transcripts. Findings revealed that substance use affected well-being, particularly patient recovery and caregiver burden. Strategies to address well-being included limiting substance use, being vigilant about patient use, and communicating the negative impact of use. The environmental impact and stigma of substance use were major contextual challenges. Patients addressed these by limiting their socialization. Family social context was important to how substance use was perceived and managed by patients and caregivers. As such, treatment models should consider a holistic perspective that incorporates family context when addressing substance use among Latinos with schizophrenia.
Automatic information extraction from childhood cancer pathology reports
Authors: Yoon Hong-Jun , Peluso Alina , Durbin Eric B , Wu Xiao-Cheng , Stroup Antoinette , Doherty Jennifer , Schwartz Stephen , Wiggins Charles , Coyle Linda , Penberthy Lynne 
Published: June 2022

Abstract

Objectives
The International Classification of Childhood Cancer (ICCC) facilitates the effective classification of a heterogeneous group of cancers in the important pediatric population. However, there has been no development of machine learning models for the ICCC classification. We developed deep learning-based information extraction models from cancer pathology reports based on the ICD-O-3 coding standard. In this article, we describe extending the models to perform ICCC classification.

Materials and Methods
We developed 2 models, ICD-O-3 classification and ICCC recoding (Model 1) and direct ICCC classification (Model 2), and 4 scenarios subject to the training sample size. We evaluated these models with a corpus consisting of 29 206 reports with age at diagnosis between 0 and 19 from 6 state cancer registries.

Results
Our findings suggest that the direct ICCC classification (Model 2) is substantially better than reusing the ICD-O-3 classification model (Model 1). Applying the uncertainty quantification mechanism to assess the confidence of the algorithm in assigning a code demonstrated that the model achieved a micro-F1 score of 0.987 while abstaining (not sufficiently confident to assign a code) on only 14.8% of ambiguous pathology reports.

Conclusions
Our experimental results suggest that the machine learning-based automatic information extraction from childhood cancer pathology reports in the ICCC is a reliable means of supplementing human annotators at state cancer registries by reading and abstracting the majority of the childhood cancer pathology reports accurately and reliably.
Improvement in the Reliability of AOAC Official MethodSM 2012.15 for Iodine, Part-2
Authors: Hieda Naoto , Nishida Motoyuki , Matsuno Ichirou 
Published: June 2022

Abstract

Background
In our previous study, the AOAC Official MethodSM2012.15 (Method 2012.15) was modified by adding carbon (as methanol) to the standard and final test solutions for accurate quantification of iodine in infant and special formulas after incomplete matrix oven digestion. However, when an inductively coupled plasma-mass spectrometry (ICP-MS) instrument from a different manufacturer was used, inaccurately low iodine content values were obtained using both the original and modified methods.

Objective
To obtain more reliable values for infant formulas using varying ICP-MS instruments.

Methods
The protocol was modified by changing the ratio of the pump tubing inner diameters (IDs) for the ICP-MS system or by optimizing the internal standards for Method 2012.15.

Results
No significant improvement was observed by changing the ratio of the pump tubing IDs. In the optimization study of the internal standard, when germanium is used as the internal standard, the measurement result of NIST SRM® 1849 was within the certified value range. Furthermore, with the addition of methanol equivalent to 5% to the standard and final test solution based on our previous study, the recovery of the samples was within the certified range of SMPR 2012.008.

Conclusions
This demonstrates that changing the internal standard to germanium and adding methanol equivalent to 5％ to the standard and final test solution, can result in stable measurement even when the ICP-MS specifications were changed.

Highlights
The modified Method 2012.15 provides reliable results even if the instrument is changed by changing the internal standard to germanium.
Adherence to Burn Resuscitation Guidelines Reduces Resuscitation Fluids and Mortality
Authors: Mankowski Peter , Papp Bettina , Genoway Krista , Papp Anthony 
Published: June 2022

Abstract
In our province, regional recommendations for optimal fluid resuscitation were published in 2011 to improve the management of acute burn patients prior to transfer to a specialized burn center. The purpose of this study was to determine compliance with these provincial burn resuscitation guidelines and their subsequent impact on patient outcomes. A retrospective review of patients transferred to the provincial burn center after being initially managed at peripheral sites was performed from 2011 to 2019. Patients were included if their burn injury was greater than 20% TBSA and they were transferred within 24 hours postburn injury. Charts were reviewed for the amount of fluid patients received and resuscitation associated outcomes. A total of 72 patients met the inclusion criteria, 37 of which were treated in accordance with the 2011 guidelines. For patients that followed the 2011 provincial guidelines, they received on average 3.2 cc/kg/TBSA of fluid during the first 24 hours postburn injury. Significantly more fluids were given when guidelines were not followed with an average of 4.4 cc/kg/TBSA (P = .03). Mortality rates were found to be significantly lower during the primary admission with guidelines compliance (16.2% vs 2.7%, P = .04). No significant differences were found between the remaining evaluated complications including abdominal compartment syndrome (8.1% vs 2.7%) and need for escharotomy (35.2% vs 21.6%). The use of clinical practice guidelines decreased excess IV fluid administration in additional to decreasing mortality rates for patients initially assessed in peripheral low volume centers.
When Products Come Alive: Interpersonal Communication Norms Induce Positive Word of Mouth for Anthropomorphized Products
Authors: Chen Fangyuan , Sengupta Jaideep , Zheng Jianqing (Frank) 
Published: June 2022

AbstractAcross five experiments, this research finds that product anthropomorphism enhances consumers’ intention to share positive thoughts in their word-of-mouth (WOM) communication about such products, in the hope of creating a favorable interpersonal impression about themselves. Our theorizing suggests that the effect occurs because consumers apply a norm that originates in human-related communication—namely, that speaking positively of other people creates a more likable impression of speakers by making them seem more kind and polite—to their WOM for anthropomorphized products (study 1). As a result, when an impression management motive is salient, consumers display greater overall positivity in their WOM for an anthropomorphized product than for its non-anthropomorphized equivalent (study 2). Support for this prediction is found across various measures of WOM positivity. Furthermore, in line with this conceptualization, anthropomorphism-induced positivity diminishes (a) when consumers are less concerned about impression management, such as when talking to a close friend (study 3), (b) when an opposing accuracy motive overpowers the impression management motive (study 4), or (c) when the underlying belief that positivity will yield favorable impressions is itself challenged (study 5). Our conceptualization and findings inform and extend research on consumer WOM communication, product anthropomorphism, and impression management.