bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Tumor Suppressor Gene-Based Prognostic Classifier Predicts Prognosis, Tumor Immune Infiltration, and Small Molecule Compounds in Breast Cancer
Authors: Jiang Suxiao , Bu Xiangjing , Tang Desheng , Yan Changsheng , Huang Yan , Fang Kun 
Published: February 2022

Objective: Tumor suppressor genes (TSGs) play critical roles in the cell cycle checkpoints and in modulating genomic stability. Here, we aimed to develop a TSG-based prognostic classifier for breast cancer.Methods: Gene expression profiles and clinical information of breast cancer were curated from TCGA (discovery set) and Gene Expression Omnibus (GEO) repository (GSE12093 and GSE17705 datasets as testing sets). Univariate cox regression analysis and random forest machine learning method were presented for screening characteristic TSGs. After multivariate cox regression analyses, a TSG-based prognostic classifier was constructed. The predictive efficacy was verified by C-index and receiver operating characteristic (ROC) curves. Meanwhile, the predictive independency was assessed through uni- and multivariate cox regression analyses and stratified analyses. Tumor immune infiltration was estimated via ESTIMATE and CIBERSORT algorithms. Small molecule agents were predicted through CMap method. Molecular subtypes were clustered based on the top 100 TSGs with the most variance.Results: A prognostic classifier including nine TSGs was established. High-risk patients were predictive of undesirable prognosis. C-index and ROC curves demonstrated its excellent predictive performance in prognosis. Also, this prognostic classifier was independent of conventional clinicopathological parameters. Low-risk patients exhibited increased infiltration levels of immune cells like T cells CD8. Totally, 48 small molecule compounds were predicted to potentially treat breast cancer. Five TSG-based molecular subtypes were finally constructed, with distinct prognosis and clinicopathological features.Conclusion: Collectively, this study provided a TSG-based prognostic classifier with the potential to predict clinical outcomes and immune infiltration in breast cancer and identified potential small molecule agents against breast cancer.
Anti-DFS70 Antibodies Are Associated With Proliferative Lupus Nephritis and Renal Pathological Activity
Authors: Chen Dandan , Zhao Li , Dai Yingxin , Du Fang , Li Enling , Niu Xiangyu , Wang Zhiqing , Zheng Bing , Lu Liangjing 
Published: February 2022

ObjectiveThe significance of anti-dense fine speckles 70 (DFS70) antibodies in systemic lupus erythematosus (SLE) is still unclear, especially in lupus nephritis (LN) patients. We investigated the prevalence, clinical and pathological relevance of anti-DFS70 antibodies in LN patients.MethodsAnti-DFS70 antibodies were measured using enzyme-linked immunosorbent assays in 377 biopsy-proven LN patients, 268 non-LN SLE patients, 232 chronic kidney disease (CKD) patients, and 78 healthy individuals (HI). Demographic, clinical, and pathological parameters were compared between LN patients with and without anti-DFS70 antibodies. Stepwise multivariable logistic regression was performed to identify covariates associated with anti-DFS70 antibodies.ResultsThe prevalence of anti-DFS70 antibodies in LN (19.6%) was comparable to non-LN SLE patients (19.8%, P=0.9630), but was significantly higher than CKD patients (13.4%, P=0.0468) and HI (9.0%, P=0.0252). Using multivariable logistic regression analysis, the titer of anti-double-stranded DNA (dsDNA) antibodies (adjusted odds ratio=1.002, 95% confidence interval 1.001-1.003, P=0.004) was associated with positive anti-DFS70 antibodies in LN patients. In addition, anti-DFS70 antibodies were more prevalent in proliferative LN (22.0%, 68/309) compared to membrane LN patients (10.2%, 6/59, P=0.0376). Furthermore, LN patients with positive anti-DFS70 antibodies had significantly higher activity index (AI) compared to patients who were negative (8.0 vs 6.0, P=0.0131). However, the chronicity index was similar between the groups (3.0 vs 3.0, P=0.8412).ConclusionAnti-DFS70 antibodies were not associated with LN development in SLE patients but were associated with anti-dsDNA antibodies, proliferative LN, and renal AI. This suggests their potential to serve as a non-histological biomarker for LN subclass and activity status.
Effect of High Strain Rates on Adiabatic Shear Bands Evolution and Mechanical Performance of Dual-Phase Ti Alloy
Authors: Hao Fang , Du Yuxuan , Wang William Yi , Mao Youchuan , Yin Junlei , Zou Chengxiong , Chen Haisheng , Wang Kaixuan , Feng Yong , Liu Xianghong , Li Jinshan 
Published: February 2022

In the present work, the adiabatic shear characteristics of our recently designed α + β dual-phase Ti alloy at different strain rates have been investigated by hat shaped specimen. The deformation process is divided into three stages: work hardening stage, steady stage, and unstable thermal softening stage. Along or near the shear deformation paths, the microvoids and the cracks can be captured at the strain rate of 1.8 × 104 s−1, 2.0 × 104 s−1, and 2.3 × 104 s−1, both of which contribute to the stable and unstable softening. It is found that dynamic stored energy of cold work will be significantly improved by the enhanced high strain rate. In the view of coupling analysis of inverse pole figure and grain boundary map, it seems that low angle grain boundaries present a good resistance to the formation of cracks and thermal softening. On the contrary, high angles grain boundaries are typically located in ASBs and their affecting regions, which is in line with the reported results. While the geometrical necessary dislocation (GND) density of adiabatic shear band (ASB) and its surroundings increased significantly, the width of the ASB becomes wider as the strain rate increases, which is consistent with the theory of sub-grain rotation dynamic recrystallization model. The formation of multiple ASBs in the corner position is schematically illustrated and the average elastic modulus and hardness of the ASB region are lower than the α and β phases, combined with the GND analysis, which proves that the ASB is a thermal softening zone in this experiment.
The Innovative Self-Sensing Strain Sensor for Asphalt Pavement Structure: Substitutability and Synergy Effects of Graphene Platelets With Carbon Nanotubes in Epoxy Composites
Authors: Xin Xue , Luan Xuehao , Su Linping , Ma Chuanyi , Liang Ming , Ding Ximao , Yao Zhanyong 
Published: February 2022

Situ sensors with high accuracy, long durability, and high survival rate are crucial for the health monitoring of asphalt pavement. Due to the harsh environment during the construction period and service life, the monitoring components which can be buried synchronously with the construction period of the road surface become a difficult problem to be solved urgently. The development of functional composites sheds a new insight for pavement strain detection with remarkable self-sensing behavior. In this paper, the substitutability and synergy effect of graphene platelets with carbon nanotubes (CNTs), the effect of CNT types with different specific surface areas in epoxy composites to the morphological, electrical, and mechanical properties, and the strain-electrical resistance response peculiarity of composites were evaluated. The performance of developed composite sensors with epoxy encapsulation was investigated through laboratory experiments. The morphologies showed that CNT-GNP hybrids in composites present a better dispersion state because of the size effect and synergetic effect whereas the pure CNTs are prone to entangle with each other. Composites with CNT(SSA500) display the most amounts of conductive units in same dosage. CNTs and GNP can strengthen the elastic modulus of the epoxy matrix to basically the same as that of asphalt mixture within the range of 1100–1500 MPa. At last, Laboratory experiments have proved the promising prospect for CNTs-GNP/epoxy composites serving as the strain sensor. The developed composites-based strain sensor can provide a new prospect for asphalt pavement monitoring.
Comparison of Various Vagal Maneuvers for Supraventricular Tachycardia by Network Meta-Analysis
Authors: Huang Edward Pei-Chuan , Chen Chi-Hsin , Fan Cheng-Yi , Sung Chih-Wei , Lai Pei Chun , Huang Yen Ta 
Published: February 2022

BackgroundVagal maneuvers (VagMs) are recommended as the first-line treatment of supraventricular tachycardia (SVT). However, the optimal type of VagMs remains unproven.AimThis study aims to compare the effectiveness and adverse events amongst VagMs on SVT via network meta-analyses (NMAs).MethodsWe systematically searched randomized controlled trials (RCTs) that involved adults with SVT and compared VagMs without language restrictions. We determined the initial and final responses of conversion rate to sinus rhythm and adverse events. Risk of bias (RoB) was appraised by Cochrane revised tool, and contribution matrix was calculated. NMAs were synthesized using frequentist random-effects model and presented as relative risk (RR) with 95% CI. The order of probability was presented as surface under the cumulative ranking curve analysis (SUCRA). Sensitivity analysis was performed using both Bayesian and frequentist approach with fixed- or random-effects models. Certainty of evidence (CoE) was rated by using the Grading of Recommendations, Assessment, Development, and Evaluations methodology.ResultsFourteen RCTs with 2,180 patients were enrolled. Small portion of mixed estimates was contributed from high overall RoB studies. Compared with carotid sinus massage (CSM), the modified Valsalva maneuver (MVM) was the most effective VagM after initial performance [SUCRA: 0.9992, RR: 5.47 (1.77–16.93)] and at the end of study [SUCRA: 1.0000, RR: 3.62 (2.04–6.39), CoE: high]. The standard VM did not elicit better conversion rate to the sinus rhythm than CSM at the initial response [SUCRA: 0.4395, RR: 1.97 (0.63–6.15)] and at the end of the study [SUCRA: 0.4795, RR: 1.64 (0.94–2.87), CoE: moderate]. The SUCRA value of CSM at the initial and final responses was the least one amongst three VagMs (0.0613 and 0.0205, respectively). Adverse events amongst three VagMs were similar (CoE: low). Sensitivity analyses yielded consistent results.ConclusionWe recommended MVM as the first choice of VagM for rhythm conversion before the pharmacological management of SVT.
Digital Dance for People With Parkinson's Disease During the COVID-19 Pandemic: A Feasibility Study
Authors: Walton Lois , Domellöf Magdalena Eriksson , Åström Åsa N. , Elowson Åsa , Neely Anna Stigsdotter 
Published: February 2022

BackgroundDance as a treatment to support physical, cognitive and emotional functioning, has gained increased acceptance as a healthcare intervention for people with Parkinson's Disease (PD). The impact of the COVID-19 pandemic has been far reaching with devastating effects for at-risk populations. To find alternative and safe treatment delivery options during the pandemic has been of utmost importance. The purpose of this mixed-methods study was to evaluate the feasibility and the experience of digital dance for people with PD (Dance for PD©) and to examine change in self-reported quality of life, psychological health, subjective cognitive complaints and mental fatigue.Methods23 participants with PD (mean age 70) partook in 10-h weekly digital Dance for PD sessions. Feasibility outcome measures were assessed at post-test. Web-based questionnaires examining quality of life, subjective memory complaints, depression, anxiety and mental fatigue were administered at pre- and post-test. Moreover, nine participants partook in focus group discussions at post-test.ResultsThe results showed an acceptable feasibility to home-based digital Dance for PD, where 86% of the dance classes were completed, only minor negative side effects were reported (i.e., sore joints), and all experienced the dance classes as motivating and safe to do at home. The majority also reported positive effects on mood and physical functioning. The results from the questionnaires showed significant improvements in depressive symptoms (p = 0.006) and quality of life (p &lt; 0.001) at post-test. In the focus groups, participants indicated that digital dance was a beneficial and enjoyable activity with a strong added value during the COVID-19 pandemic. Nevertheless, they experienced that digital dance missed some important elements of live dance.ConclusionsThis study showed that digital Dance for PD is feasible and holds promise as a viable and safe method to keep people with PD dancing even when physical meetings are not possible. Beyond the pandemic, digital dance could be applied to a wide variety of patient groups including rural populations and patients for whom transportation may not be feasible for practical or financial reasons.Trial RegistrationRetrospectively registered at ClinicalTrials.gov on 25/06/2021 with the following registration number: NCT04942392.
Plausible Mechanism of Sham Acupuncture Based on Biomarkers: A Systematic Review of Randomized Controlled Trials
Authors: Kim Tae-Hun , Lee Myeong Soo , Birch Stephen , Alraek Terje 
Published: February 2022

IntroductionSham acupuncture was developed to be used as an inert control intervention in clinical trials of acupuncture. However, controversies exist regarding the validity of sham acupuncture. In this systematic review (SR) of acupuncture trials, we assessed whether serum biomarkers showed significant differences after sham and verum acupuncture treatments.MethodsAny acupuncture clinical trials that evaluated serum biomarker changes between sham acupuncture and verum acupuncture were included in this review. Relevant literature was searched in the PubMed database, EMBASE, and The Cochrane Central Register of Controlled Trials (CENTRAL) database from inception until June 2021. The Cochrane risk of bias was assessed. Summary effect estimates for each biomarker between groups were calculated with a random effect model.ResultsFrom 51 sham acupuncture trials, we found that there were no significant differences in most of the 36 serum biomarkers after sham acupuncture and verum acupuncture needling. Only VEGF, IG-E, TNF-a, NGF, GABA, NPY, and VIP serum levels were identified as being different between the groups. The overall risk of bias of the included studies and the limited numbers of studies for meta-analysis do not strongly support the results of this SR.ConclusionSham acupuncture techniques might have similar effects on biomarkers as the so-called “real acupuncture” techniques, which indicates that sham acupuncture, as an inert intervention similar to a placebo drug, needs to be reconsidered.Systematic Review PROSPERO Registrationidentifier [CRD42021260889].
Association Between Dietary Zinc Intake and Metabolic Syndrome. A Meta-Analysis of Observational Studies
Authors: Ding Jun , Liu Qi , Liu Ze , Guo Hongbin , Liang Jieyu , Zhang Yi 
Published: February 2022

BackgroundEpidemiological studies have investigated the association between dietary zinc intake and metabolic syndrome (MetS). However, their results are conflicting. This meta-analysis was therefore employed to investigate the associations further.MethodsA comprehensive literature search was employed by using the electronic database of PubMed, Web of Science, and Embase up to November 2021. The pooled relative risk (RR) of MetS for the highest vs. lowest dietary zinc intake category, and the weighted mean difference (WMD) of dietary zinc intake for MetS vs. control subjects as well as their corresponding 95% confidence interval (CI) were calculated.ResultsA total of 13 observational studies (18,073 participants) were identified in this meta-analysis. The overall multi-variable adjusted RR demonstrated that the dietary zinc intake was inversely associated with MetS (RR = 0.75, 95%CI: 0.61 to 0.93; P = 0.009). The subgroup analysis confirmed such findings in cross-sectional (RR = 0.70, 95%CI: 0.55 to 0.87; P = 0.002), NCEP-ATP III (RR = 0.64, 95%CI: 0.48 to 0.84; P = 0.002), adult (RR = 0.77, 95%CI: 0.62 to 0.96; P = 0.02), dietary recall method (RR = 0.70, 95%CI: 0.55 to 0.87; P = 0.002), and &gt;500 sample-sized study (RR = 0.79, 95%CI: 0.64 to 0.99; P = 0.002), respectively. On the other hand, the overall combined WMD showed that the dietary zinc intake in MetS was also lower than that in control subjects (WMD = −0.21, 95%CI: −0.42 to 0.00; P = 0.05).ConclusionsOur results suggest that the dietary zinc intake is negatively associated with MetS. However, due to the limitation of available evidence. More well-designed prospective cohort studies are still needed.
A Regression Model to Predict Linezolid Induced Thrombocytopenia in Neonatal Sepsis Patients: A Ten-Year Retrospective Cohort Study
Authors: Duan Lufen , Zhou Qin , Feng Zongtai , Zhu Chenqi , Cai Yan , Wang Sannan , Zhu Meiying , Li Jingjing , Yuan Yunlong , Liu Xin , Sun Jiantong , Yang Zuming , Tang Lian 
Published: February 2022

Background: Linezolid-induced thrombocytopenia (LIT) is the main factor limiting the clinical application of linezolid (LZD). The incidence and risk factors of LIT in neonatal patients were possibly different from other populations based on pathophysiological characteristics. The purpose of this study was to establish a regression model for predicting LIT in neonatal sepsis patients.Methods: We retrospectively included 518 patients and divided them into the LIT group and the non-LIT group. A logistic regression analysis was used to analyze the factors related to LIT, and a regression model was established. A receiver operating characteristic (ROC) curve was drawn to evaluate the model’s predictive value. We prospectively collected 39 patients’ data to validate the model and evaluate the effect of LZD pharmacokinetics on LIT.Results: Among the 518 patients, 103 patients (19.9%) developed LIT. The Kaplan–Meier plot revealed that the overall median time from the initiation of LZD treatment to the onset of LIT in preterm infants was much shorter when compared with term infants [10 (6, 12) vs. 13 (9.75, 16.5), p = 0.004]. Multiple logistic regression analysis indicated that the independent risk factors of LIT were lower weight at medication, younger gestational ages, late-onset sepsis, necrotizing enterocolitis, mechanical ventilation, longer durations of LZD treatment, and lower baseline of platelet level. We established the above seven-variable prediction regression model and calculated the predictive probability. The ROC curve showed that the predicted probability of combined body weight, gestational age, duration of LZD treatment, and baseline of platelet had better sensitivity (84.4%), specificity (74.2%), and maximum AUC (AUC = 0.873). LIT occurred in 9 out of 39 patients (23.1%), and the accuracies of positive and negative predictions of LIT were 88.9 and 76.7%, respectively. Compared with the non-LIT patients, the LIT patients had higher trough concentration [11.49 (6.86, 15.13) vs. 5.51 (2.80, 11.61) mg/L; p = 0.028] but lower apparent volume of distribution (Vd) [0.778 (0.687, 1.421) vs. 1.322 (1.099, 1.610) L; p = 0.010].Conclusion: The incidence of LIT was high in neonatal sepsis patients, especially in preterm infants. LIT occurred earlier in preterm infants than in term infants. The regression model of seven variables had a high predictive value for predicting LIT. LIT was correlated with higher trough concentration and lower Vd.
Cannabidiol Inhibits Multiple Ion Channels in Rabbit Ventricular Cardiomyocytes
Authors: Isaev Dmytro , Shabbir Waheed , Dinc Ege Y. , Lorke Dietrich E , Petroianu Georg , Oz Murat 
Published: February 2022

Cannabidiol (CBD), a major non-psychotropic cannabinoid found in the Cannabis plant, has been shown to exert anti-nociceptive, anti-psychotic, and anti-convulsant effects and to also influence the cardiovascular system. In this study, the effects of CBD on major ion currents were investigated using the patch-clamp technique in rabbit ventricular myocytes. CBD inhibited voltage-gated Na+ and Ca2+ channels with IC50 values of 5.4 and 4.8 µM, respectively. In addition, CBD, at lower concentrations, suppressed ion currents mediated by rapidly and slowly activated delayed rectifier K+ channels with IC50 of 2.4 and 2.1 µM, respectively. CBD, up to 10 μM, did not have any significant effect on inward rectifier IK1 and transient outward Ito currents. The effects of CBD on these currents developed gradually, reaching steady-state levels within 5–8 min, and recoveries were usually slow and partial. Hill coefficients higher than unity in concentration-inhibition curves suggested multiple CBD binding sites on these channels. These findings indicate that CBD affects cardiac electrophysiology by acting on a diverse range of ion channels and suggest that caution should be exercised when CBD is administered to carriers of cardiac channelopathies or to individuals using drugs known to affect the rhythm or the contractility of the heart.
Evaluation of JQ1 Combined With Docetaxel for the Treatment of Prostate Cancer Cells in 2D- and 3D-Culture Systems
Authors: Xu Yipeng , Pachnikova Gabriela , Przybilla Dorothea , Schäfer Reinhold , Cui Yingying , Zhou Dan , Chen Zihao , Zhao An , Keilholz Ulrich 
Published: February 2022

Introduction: Prostate cancer (PCa) is dependent on coupled androgen-androgen receptor (AR) signaling for growth and progression. Significant efforts have been made in this research field, as hormonal therapies have greatly improved the survival of patients with metastatic PCa (mPCa). The drug treatment agent JQ1, which potently abrogates bromodomain 4 (BRD4) localization to the AR target loci and therefore significantly impairs AR-mediated gene transcription, is a potent therapeutic option for patients with advanced PCa. In this study, we aimed to investigate the inhibitory effect of JQ1 combined with docetaxel on PCa cells in vitro for the first time. Furthermore, the 3D spheroid culture system was modeled to more accurately simulate the response of PCa cells to drugs.Methods: We established and measured 3D LNCaP spheroids in vitro in order to evaluate the susceptibility of 2D- and 3D-cultured LNCaP cells exposed to the same anti-cancer drug.Results: We demonstrated that JQ1 was an effective drug for promoting cell inhibition after docetaxel treatment in 2D- and 3D- cultured LNCaP cells. Inhibition of 3D cultured formation in the combined treatment group was significantly higher than that in docetaxel or JQ1 alone. Under the same conditions of drug solubility, the drug resistance of 3D spheroids was significantly higher than that of 2D cells. Moreover, dmax and lg volume were suitable parameters for LNCaP cells/spheroid size displaying and evaluating cell viability.Conclusion: 3D cultured spheroids of PCa are an effective tool for studying PCa drug trials. JQ1 combined with docetaxel may be an effective treatment for advanced PCa. This combination therapy strategy deserves further evaluation in clinical trials.