bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pollution load assessment of agricultural soil samples of some villages from Ludhiana district, Punjab.
Authors: Sharma Priyanka , Kaur Jatinder 
Published: March 2022

Abstract
Aims Continuous use of inorganic fertilizers, pesticides, wastewater discharge, and leachates causes soil degradation, contamination of potable water and food ultimately leading to soil pollution and ill effects on human health. The current study involves monitoring of soil quality of agricultural soil samples collected from four different agricultural fields in Ludhiana, Punjab (India) near Buddha Nullah, a tributary of the Sutlej river. Methods Physico-chemical characteristics and heavy metal content of soil samples were estimated following the standard protocols. The genotoxic potential of soil samples were evaluated by using Allium cepa root chromosomal aberration assay.Results The agricultural soil samples were found to be slightly basic. Soil nutrients such as nitrate, phosphate and potassium ranged from 0.072 - 0.075 mg/g, 0.029 - 0.070 mg/g and 0.055 - 0.158 mg/g respectively. The contents (mg/kg) of heavy metals cadmium, chromium, cobalt, copper, and lead analyzed in five of the soil samples were observed to be above the permissible limits. Allium cepa root chromosomal aberration assay used for genotoxicity studies has shown that Hambran (HBN), induced maximum genotoxic effect, i.e. 46.7% in terms of percent aberrant cells in Allium cepa. Conclusions The statistical analysis evaluated the positive correlation of heavy metals (Cu, Co, Ni) with the percentage chromosomal aberration induced in Allium cepa test. The Pearson correlation indicated that genotoxicity had a significant positive correlation with the content of Cu and Ni at p ≤ 0.05 and with Co at p ≤ 0.01.
Alternations of fecal microbes and lung tissue microbes in Lung Cancer patients is more Identical than that of sputum
Authors: Li Yanze , Kun Huang , Zhang Hao 
Published: March 2022

Abstract
Microbiome in the human body environment is related to the occurrence of a variety of disease phenotypes. In this project, we collected lung tissue, feces and sputum from three Bioprojects of NCBI related to lung cancer (LC). Each project contains LC cases and lung normal (LN) controls. Those three projects contain a total of 339 samples of 16s rRNA sequencing data. By analyzing the composition of microbes in the three environments, and predicting their functions we found that compared with sputum, the ecological environment of fecal microbe is closer to tissue microbes in terms of evolutionary relationship. We used Picrust2 to predict the differential microbe function of lung cancer (LC) and the control (Lung Normal, LN) groups in the three environments, and found that at the microbe genetic level, compared to feces and sputum, sputum and tissues, feces and tissues have more common Differential genes, at the level of differential enzyme genes and differential pathways, feces and tissues have more common differences compared to feces and sputum, sputum and tissues. The results indicated that the activation of marker genus in sputum and feces and significantly changed pathways has an opposite trend, and there are many pathways contributing to glycolysis are correlated with marker genus. Patients with LC has potential to regulate the microbe composition of feces, tissues and sputum by regulating glycolysis metabolism.
Neural network-based prediction of the secret-key rate of quantum key distribution
Authors: Yin Hua-Lei , Zhou Min-Gang , Liu Zhi-Ping , Liu Wen-Bo , Li Chen-Long , Bai Jun-Lin , Xue Yi-Ran , Fu Yao , Chen Zeng-Bing 
Published: March 2022

Abstract
Numerical methods are widely used to calculate the secure key rate of many quantum key distribution protocols in practice, but they consumes many computing resources and are too time-consuming. In this work, we take the homodyne detection discrete-modulated continuous-variable quantum key distribution (CV-QKD) as an example, and construct a neural network that can quickly predict the secure key rate based on the experimental parameters and experimental results. Compared to traditional numerical methods, the speed of the neural network is improved by several orders of magnitude. Importantly, the predicted key rates are not only highly accurate but also highly likely to be secure. This allows the secure key rate of discrete-modulated CV-QKD to be extracted in real time on a low-power platform. Furthermore, our method is versatile and can be extended to quickly calculate the complex secure key rates of various other unstructured quantum key distribution protocols.
A Variety of Surface Techniques, Several Denture Base Repair Properties Were Investigated
Authors: Alfahdawi Ibrahim 
Published: March 2022

Abstract
Heat-cure acrylic resin is used, and six sets of specimens were cut in guiding mode with a conventional positioning clamp. To evaluate impact strength, silicon sheets with diameters of (50×5×4) mm were created. On an alternative bending fatigue machine, specimens with a diameter of (70× 10 ×2.5) mm were evaluated. The monomer solvent's impact bond strength was found to be higher than that of the thinner solvent and the acetone solvent. Thiner solvents and monomer solvents have a lower fatigue bond strength than acetone.
Non-cystic macular thickening on optical coherence tomography as an alternative to fluorescein angiography for predicting retinal vascular leakage in early stages of uveitis
Authors: Ebrahimiadib Nazanin , Kianzad Zahra , Zarei Mohammad , Davoudi Samaneh , Riazi-Esfahani Hamid , Bazvand Fatemeh , Mahdizad Zahra , Modjtahedi Bobeck S 
Published: March 2022

Abstract
Purpose: To evaluate the relationship between non-cystic thickening of the macula on optical coherence tomography (OCT) and retinal vascular leakage on fluorescein angiogram (FA) in patients with uveitis.Method: A cross-sectional study of patients seen at the uveitis clinic was conducted. The correlation between OCT features and the degree of anterior chamber and anterior vitreous cell, best corrected visual acuity (BCVA), and FA findings were examined. Results: A total of 100 exam data met inclusion criteria. There was a significant relationship between macular volume as well as central macular thickness (CMT) and the mean 3mm and 6mm perifoveal macular thickness with angiographic scores (all P values<0.0001). Regarding the colors in thickness map, colors of red and orange which is indicative of thickening were more frequently observed in the area between 1 and 3mm ring. Conclusion: Non-cystic thickening of the macula on OCT, especially in perifoveal area, is a reliable predictor of the presence of retinal vascular leakage in patients with uveitis.
Spring-Dashpot Vibrational Model for the Investigation of Viscoelasticity in Gelatinous Abrasive Media and Subsequent Control of Parameters for the Blast Polishing of Ti-6Al-4V Alloy
Authors: de Jongh Quintin Oliver , Kuppuswamy Ramesh , Titus Matthew 
Published: March 2022

Abstract
Blast polishing offers an operator-friendly solution to many of the previously encountered polishing difficulties. However, the process lacks studies into the control of key parameters, one of which is viscoelasticity (particularly present in biological based abrasive medias). Together with analytical-empirical models, a vibrational spring-dashpot model is presented, which attempts to characterize the impact force, contact time and damping ratio/coefficient of polishing media upon impact; as well as the effects of damping on contact parameters (stress, deformation, and area of contact). Impact force is shown to decrease dramatically with increasing hydration but increases linearly with an increase in kinetic energy. Contact time results show an exponential increase as hydration is increased and show a logarithmic decrease (to a limit) as kinetic energy is increased. Findings show that higher hydration levels result in lower damping ratios (with all results showing that underdamping is present). Higher kinetic energies show a decrease in damping ratio. Similarly, media damping coefficients decrease with both hydration increases and kinetic energy increases. Results show that contact stress is reduced at higher hydration levels (due to higher contact areas and lower forces) and that hydration acts to prevent brittle failure from occurring on the workpiece surface. The findings stipulated provide a base on which to further characterize the process and will help in development and further optimization of the blast polishing process.
Blood donor biobank and HLA imputation as a resource of HLA homozygous cells for therapeutic and research use
Authors: Clancy Jonna , Hyvärinen Kati , Ritari Jarmo , Wahlfors Tiina , Partanen Jukka , Koskela Satu 
Published: March 2022

Abstract
BackgroundAllogeneic therapeutic cells may be rejected if they express HLA alleles not found in the recipient. As finding cell donors with a full HLA match to a recipient requires vast donor pools, use of HLA homozygous cells has been suggested as an alternative. HLA homozygous cells should be well tolerated by those who carry at least one copy of donor HLA alleles. HLA-A-B homozygotes could be valuable for HLA-matched thrombocyte products. We evaluated the feasibility of blood donor biobank and HLA imputation for identification of potential cell donors homozygous for HLA alleles.MethodsWe imputed HLA-A, - B, -C, -DRB1, -DQA1, -DQB1, and -DPB1 alleles from genotypes of 20,737 Finnish blood donors in the Blood Service Biobank. We confirmed homozygosity by sequencing HLA alleles in 30 samples and by examining 36,161 MHC-located polymorphic DNA markers. Results317 individuals (1.5%), representing 41 different haplotypes, were found to be homozygous for HLA-A, -B, -C, -DRB1, -DQA1 and -DQB1 alleles. Ten most frequent haplotypes homozygous for HLA-A to -DQB1 were HLA-compatible to 49.5% and three most frequent homozygotes to 30.4% of the Finnish population. Ten most frequent HLA-A-B homozygotes were compatible to 75.3% and three most frequent haplotypes to 42.6% of the Finnish population. HLA homozygotes had a low level of heterozygosity in MHC-located DNA markers, in particular in HLA haplotypes enriched in Finland.ConclusionsThe present study shows that HLA imputation in a blood donor biobank of reasonable size can be used to identify HLA homozygous blood donors suitable for cell therapy, HLA-typed thrombocytes and research. The homozygotes were HLA-compatible to a large fraction of the Finnish population. Regular blood donors reported to have positive attitude to research donation appear a good option for these purposes. Differences in population frequencies of HLA haplotypes emphasize the need for population specific collections of HLA homozygous samples.
Characterization of the first Rubus yellow net virus genome from blackberry
Authors: Vakic Misaela , Stainton Daisy , Delic Duska , Tzanetakis Ioannis E. 
Published: March 2022

Abstract
Rubus yellow net virus (RYNV) is a badnavirus that infects Rubus spp. In mixed infections with black raspberry necrosis and raspberry leaf mottle they cause raspberry mosaic, a disease that leads to significant losses and even plant death. RYNV has been reported in several European countries and the Americas yet there is substantial lack of knowledge; especially when it comes to virus diversity and the evolutionary forces that affect virus fitness outside its primary host, raspberry. Herein we report the first RYNV episomal genome isolated from blackberry and this is the first report of the virus in Bosnia and Herzegovina. The isolate has five ORFs and when compared with other fully sequenced counterparts showed 82-97% nucleotide pairwise identity. This communication adds to our limited knowledge on RYNV and addresses some of the knowledge gaps in RYNV genetics.
Coupling characteristics of creep fracture of rock foundation on wind turbine under wind induced vibration
Authors: wang jun , nie zhipeng , cao ping 
Published: March 2022

Abstract

This paper presents the elastoplastic creep fracture characteristics and bearing stability of rock foundation for wind turbine based on the influence of wind load. Combined with the superstructure-concrete foundation-rock foundation lay load and boundary conditions, the wind load standard value and wind turbine system composition were obtained under normal wind condition, and then the forced vibration model of viscous damping two-degree-of-freedom system was established. Furthermore, the mode characteristics, frequency characteristic equations and the relations of the first and second order natural frequencies were obtained. According to the plastic yield characteristics of power hard rock base material, the analytical expressions of displacement, plastic zone and plastic state of I-II composite crack were obtained by coupling Mohr-Coulomb plastic yield condition and creep fracture characteristics. Simultaneously, the improved nonlinear creep model equation and accelerated creep fracture time were also obtained based on improved Kelvin nonlinear accelerated creep model. Combined with the example, it is verified that the accelerated creep displacement and crack propagation of rock foundation are obvious, considering the wind bracing and creep characteristics of rock foundation. Final, the failure modes of rock-foundation are compressive shear, local shear and bending-shear.It is necessary to timely reinforce the interface of foundation rock foundation.
Enlarged unilateral posterior vertebral column resection for spinal kyphosis accompanied with compressive vertebral fracture: short term clinical outcome with one-year follow-up
Authors: Guo Lei , Li Jiaqi , Zhang Fei , Zhang Peng , Sun Yapeng , Zhang Wei 
Published: March 2022

Abstract
Objective: The aim of this study was to evaluate the clinical outcome of a modified surgical technique, enlarged unilateral posterior vertebral column resection (EU-PVCR), for treatment of spinal kyphosis accompanied with compressive vertebral fracture.Material and Methods: Between January 2015 and December 2020, data of 13 patients who underwent EU-PVCR were retrospectively collected. All patients were followed up for a minimum of 12 months. Clinical outcome was evaluated by kyphotic Cobb’s angle, sagittal vertical axis (SVA), visual analogue scale (VAS) score and Oswestry disability index (ODI). The operation time, blood loss and complications were also recorded.Results: All patients received this operation successfully. The average time of follow up was 12.3±2.1 months. At pre-operation, 1 week post-operation and 12 months post-operation, the Cobb angle were 45.5±6.1, 7.9±1.3, 8.6±1.4 (P<0.05). SVA was 3.2±0.8cm at pre-operation, and changed to 1.9±0.9cm at post-operation, and 2.1±1.1cm at the last follow up. At pre-operation, 1 weeks post-operation and 12 months post-operation, VAS were 6.1±1.3, 2.5±0.5, 1.3±0.5 (P<0.05).ODI was 28.0±2.7 before operation, and improved to 8.9±1.9 at most recent follow-up (P<0.05). No permanent neurological complication was observed during follow up.Conclusion: EU-PVCR technique is an effective surgical method for the treatment of spinal kyphosis accompanied by compressive vertebral fracture in adults, while the long-term clinical outcome remains further investigated.
Non-Thermal Atmospheric Pressure Plasma promotes healing of excisional skin wounds in caloric restricted rats
Authors: Lima Anadhelly Cristina Silva , Alves Naiara , Hummel Lucas Henrique , Lopes Bruno Bellotti , Bittencourt João Vitor Silvano , Casagrande Laura Roch , Silveira Paulo Cesar Lock , Amaral Maria Esméria Corezola , Aro Andrea Aparecida , Andrade Thiago Antônio Moretti 
Published: March 2022

Abstract
Therapeutic strategies to accelerate healing remain crucial, particularly during caloric restriction (CR), as nutritional deficiency can delay healing. Non-Thermal Atmospheric Pressure Plasma (AP) has been highlighted as appropriate therapy for tissue healing, showing pioneering results associated with 30% CR. Herein, 40 male Wistar rats with 30% CR and excisional lesions, created using 1.5 cm diameter dermatological punch, were divided into sham (lesions treated with the equipment turned off) and AP (topical and daily treatment with AP for 60s) groups. Animals were euthanized on 2nd, 7th, 14th and 21st days post-injury, and skin/lesion/scar samples were harvested for analyses: histomorphometric (inflammatory infiltrate, blood vessels, fibroblasts, collagen and hair follicles), biochemical [(myeloperoxidase–neutrophil, N-acetylglucosaminidase–macrophage), oxidative stress (nitric oxide, dichlorofluorescein, carbonyl, sulfhydryl, superoxide dismutase, and glutathione)], molecular (interleukin [IL]-1β, IL-10, transforming growth factor [TGF]-β1, vascular epithelial growth factor [VEGF], collagen III and I), and re-epithelialization (wound healing rate). The AP group showed better results than the sham group, with elevated levels of inflammatory infiltrate, IL-1β, and IL-10. AP treatment modulated oxidative stress and enhanced TGF-β1 and VEGF levels, presenting increased hair follicles, fibroblasts, and collagen I and III, promoting wound re-epithelialization. Therefore, AP could be a promising therapy for wound healing, even during 30% CR.
A Novel multifunctional 5,6-dimethoxy-indanone-chalcone-carbamate hybrids alleviates cognitive decline in Alzheimer’s disease by dual inhibition of acetylcholinesterase and inflammation
Authors: Liu Chan , Sang Zhipei , Pan Hong , Wu Qin , Qiu Yu , Shi Jingshan 
Published: March 2022

Abstract
Backgrounds: Alzheimer's disease (AD) is a multifactorial neurodegenerative disease. The treatment of AD through multiple pathological targets may generate therapeutic efficacy better. The multifunctional molecules that simultaneously hit several pathological targets have been of great interest in intervention of AD. Methods: Here we combined the chalcone scaffold with carbamate moiety and 5,6-dimethoxy-indanone moiety to generate a novel multi-target-directed ligand (MTDL) molecule(E)-3-((5,6-dimethoxy-1-oxo-1,3-dihydro-2H-inden-2-ylidene)-methyl)phenylethyl(methyl) carbamate (named AP5). In silico approaches were used to virtually predict the binding interaction of AP5 with AChE, the drug-likeness and BBB penetrance, and later validated by evaluation of pharmacokinetics (PK) in vivo by LC-MS/MS. Moreover, studies were conducted to examine the potential of AP5 for inhibiting AChE and AChE-induced amyloid-β (Aβ) aggregation, attenuating neuroinflammation and providing neuroprotection in the APP/PS1 model of AD. Results: We found that AP5 can simultaneously bind to the peripheral and catalytic sites of AChE by molecular docking. AP5 exhibited desirable pharmacokinetics (PK) characteristics including oral bioavailability (67.2%), > 10% brain penetrance and favorable drug-likeness. AP5 inhibited AChE activity and AChE-induced Aβ aggregation in vivo and in vitro. Further, AP5 lowered Aβ plaque deposition and insoluable Aβ levels in APP/PS1 mice. Moreover, AP5 exerted anti-inflammatory responses by switching microglia to a disease-associated microglia (DAM) phenotype and preventing A1 astrocytes formation. The phagocytic activity of microglial cells to Aβ was recovered upon AP5 treatment. Importantly, chronic AP5 treatment significantly prevented neuronal and synaptic damage and memory deficits in AD mice. Conclusion: Together, our work demonstrated that AP5 inhibited the AChE activity, decreased Aβ plaque deposition by interfering Aβ aggregation and promoting microglial Aβ phagocytosis, and suppressed inflammation, thereby rescuing neuronal and synaptic damage and relieving cognitive decline. Thus, AP5 can be a new promising candidate for the treatment of AD
Identification of Novel Major Quantitative Trait Loci Associated with Selenium and Zinc Accumulation in Aegilops tauschii
Authors: Chen Hong , Liu Tao , Zhou Rangrang , Ning Shunzong , Zhang Lianquan , Zhang Bo 
Published: March 2022

Abstract
Background and aims Wheat is the major nutrient source for numerous people. Aegilops tauschii Coss. (DD), the D genome donor of common wheat (BBAADD), exhibits abundant genetic variations in selenium and zinc concentrations. Ae. tauschii accession AS2407 exhibited prominent Se and Zn accumulation ability. The objectives of this study were to map Se and Zn accumulation-related quantitative trait loci (QTLs) and to identify candidate genes controlling Se and Zn accumulation in Ae. tauschii. Methods In this study, quantitative trait locus (QTL) mapping was carried out for selenium and zinc accumulation in the aerial parts of recombinant inbred lines (RILs) derived from two subspecies, AS2407 (Ae. tauschii. ssp. strangulate) and AS65 (Ae. tauschii. ssp. tauchii), and candidate genes were screened. Kompetitive allele-specific polymerase chain reaction (KASP) markers, which are closely linked to QTLs, were developed, and their distributions were detected in a natural population of Ae. tauschii. Results QTLs controlling selenium and zinc content were identified on chromosomes 1D and 2D, named QSe-1D and QZn-2D, respectively, which could explain 11.94% and 13.49% of the phenotypic variation, respectively. Five selenium-related candidate genes and three zinc-related candidate genes were screened. KASP markers (KASP_Chr1_22217614 and KASP_Chr2_57200260) were developed. QSe-1D and QZn-2D are the first mapped QTLs controlling selenium and zinc accumulation in the aerial part of Ae. tauschii. Conclusions These QTLs and KASP markers will be useful for quality improvement of wheat high nutrition and valuable for understanding the biological basis of Se and Zn intake in Ae. tauschii.
High neutrophils and triglycerides lead to critical illness in COVID-19 and reveal CDK6 inhibitors as potential treatment
Authors: Schmidt Marco F. , Baukmann Hannes A. , Cope Justin L. , Ravarani Charles N. J. , Bannard Colin , Lamparter Margaretha R. J. , Schwinges Alexander R. E. C. , Klinger Joern E. 
Published: March 2022

Abstract
Despite the recent development of vaccines and monoclonal antibodies preventing SARS-CoV-2 infection, treating critically ill COVID-19 patients still remains a top goal. In principle, drug repurposing -- the use of an already existing drug for a new indication -- could provide a shortcut to a treatment. However, drug repurposing is often very speculative due to lack of clinical evidence. We report here on a methodology to find and test drug target candidates for drug repurposing. Using UK Biobank data, we screened for significant differences in 33 blood cell types, 30 blood biochemistries, and body mass index between an infectious disease phenotype and healthy controls. We then matched critically ill COVID-19 cases with controls that exhibited mild or no symptoms after SARS-CoV-2 infection. Using data from the UK Biobank, we describe a workflow to find evidence for high neutrophil cell count and high concentrations of blood triglycerides as predictors of the immune overreaction in critical illness due to COVID-19. Based on these findings, we identified the enzyme CDK6 as a potential drug target to prevent in high risk individuals with high neutrophil cell count the immune overreaction in critical illness due to COVID-19. Three existing CDK4/6 inhibitors -- abemaciclib, ribociclib, and palbociclib -- have been approved for the treatment of breast cancer. Clinical evidence for CDK4/6 inhibitors in treating critically ill COVID-19 patients has been reported. Further clinical investigations are ongoing.