bluuebunny/crossref_metadata_2025_split huggingface dataset:
132 Ranking Beef Heifers for Residual Intake Using an Unprocessed Grass Hay Diet and Its Relationship to Greenhouse Gasses
Authors: Briggs Emma A , Holder Amanda , Moore Mikayla F , Lalman David 
Published: March 2022

Abstract
The objective of this study was to rank beef heifers for residual feed intake and greenhouse gas emissions using an unprocessed, grass hay diet. Fifty yearling Angus heifers were monitored for 62 days using the SmartFeed individual intake measuring technology (C-Lock Inc., Rapid City, SD) in drylot pens at the Oklahoma State University Range Cow Research Center located in Stillwater, OK. Means ± SD for dry matter intake (DMI; 6.76 ± 1.20 kg), average daily gain (ADG; 0.51 ± 0.21 kg), body weight (BW; 338.67 ± 55.24 kg), and residual feed intake (RFI; -0.04 ± 0.80 kg) were recorded while the heifers were fed unprocessed long-stem grass hay (10.3% CP, 2.0 Mcal ME, and 56% TDN). Additionally, a subset of 29 heifers were subjected to the collection of gas flux data using an open-circuit, portable, gas-quantification system (GreenFeed, C-Lock Inc., Rapid City, SD) to measure (means ± SD) oxygen consumption (O2; 3979.51 ± 510.32 g), carbon dioxide production (CO2; 5299.65 ± 610.89 g), and methane production (CH4; 202.656 ± 28.30 g). Using R, RFI was calculated using a multiple regression equation to predict DMI using BW0.75 and ADG (P &gt; 0.05; R2 = 0.37). To analyze forage utilization efficiency, heifers were classified into three RFI categories consisting of efficient (n = 16), average (n = 16), and inefficient (n =18). The ADG was low for all RFI classifications and similar for all three categories (P &lt; 0.05). Heifers classified in the top third of RFI rank consumed 22% less forage per day compared to the inefficient heifers. The phenotypic correlation between DMI with CO2, CH4, and O2 was 0.66, 0.18, and 0.49, respectively. These data suggest that progress in feed efficiency can be achieved when cattle are tested using a moderate-quality, unprocessed forage diet.
87 Probiotic Supplementation Improves Lamb Performance When Transitioning to High Concentrate Diets
Authors: Moody Jordan N , Whitsel Whitney , Ramsey W S , Redden Reid 
Published: March 2022

Abstract
Lamb ruminal function is integral to their health and performance. To enhance growth post-weaning, lambs are often fed a high concentrate diet. While this strategy is common, issues can arise with ruminal acidosis from excess grain consumption, including reduced weight gain and mortality. We hypothesized that administering probiotics orally prior to the transition to concentrate feeds would improve lamb performance. To assess this, finewool lambs (n = 103; males: n = 47, females: n = 56) were weaned from their dams at approximately 100 days of age at an average body weight (BW) of 25.8 ±0.2 kg and placed in a feedlot. Lambs were randomly assigned to treatments, balanced by sex, of 0g, 5g, or 10g of an orally administered probiotic paste (ProBios, Menomonie, WI) containing live lactate producing bacteria. Treatments were administered upon arrival at the feedlot. Lambs were limit fed for 9 days and gradually transitioned to ad libitum access to a high concentrate ration. At 6 weekly intervals, BW, average daily gain (ADG) and blood plasma were collected. Mixed model analysis, with initial weight as a covariate, revealed that lambs receiving 10g of supplement were significantly heavier (P = 0.01) than 5g and 0g treated lambs (30.3±0.2 kg versus 29.9±0.2 kg and 29.6±0.2 kg). ADG was analyzed with fixed effects of treatment and sex. Analysis revealed that ADG tended to be greater for lambs receiving 10g treatment than those receiving 5g or 0g (P = 0.07). Four incidences of mortality were recorded; however there were no significant treatment or sex effects on lamb mortality. These data suggest that probiotic supplementation may be an effective strategy to decrease detrimental effects of diet transition on feeder lambs and may increase lamb performance while transitioning to a high concentrate diet.
125 Utilization of Performance-tested and NSIP Sires Improved Progeny Performance and Value in Ram Test Program
Authors: Weaver Andrew R , Wright Donald L , Greiner Scott P 
Published: March 2022

Abstract
Sire selection can have long-term impacts on flock performance. The objective here was to evaluate the effect of performance-tested and/or NSIP rams on progeny performance and value in a ram test program. Rams (Katahdin = 104, Dorper = 7, Texel = 3, Cheviot = 3) were delivered to the Southwest Virginia Agricultural Research and Extension Center (SWAREC) Ram Test on June 1. Ram pedigrees were obtained from consignors and rams were classified based on whether their sires were tested in a previous SWAREC Ram Test (TESTED), enrolled in the National Sheep Improvement Program (NSIP), or neither TESTED nor NSIP (NEITHER). Some sires (16%) were previously tested and enrolled in NSIP. Rams were dewormed and rested for two weeks. On June 22, rams were given Haemonchus contortus larvae adjusted for body weight (average = 5000 L3). FAMACHA scores and fecal egg counts (FEC) were monitored every two weeks until August 31. Rams were dewormed based on FAMACHA ≥ 3. Select rams were sold at auction as breeding stock. Statistical analysis were performed using SAS (SAS Institute, Cary, NC). Rams requiring deworming had greater FAMACHA scores and FEC at all time points (P &lt; 0.05), despite not requiring treatment until August 17 (5%) and August 31 (12%). In total, 17% of rams were dewormed. Of the dewormed rams, a smaller portion of rams sired by TESTED rams (10%) and NSIP rams (15%) required treatment compared to rams sired by NEITHER rams (85%; P &lt; 0.05). Rams sired by TESTED and NSIP rams had a higher average value than those sired by NEITHER rams ($2070 and $1838, respectively, vs. $1078; P &lt; 0.05). Selection of sires that are TESTED and/or NSIP resulted in progeny that had a lesser chance of requiring treatment and sold for greater value.
31 The Effect of Increasing Inclusion of Hempseed Meal on Fiber Digestibility and Performance in Meat Rabbits
Authors: Verret Emily G , Smith W Brandon , Runyan Cheyenne L , Jacobson K June June , Owsley Frank F , Kinman Lea Ann A , Muir James P P 
Published: March 2022

Abstract
Legalization of hemp production in the United States shows potential for utilizing hempseed meal (HSM) as an effective byproduct feedstuff. Our objective was to determine total-tract fiber digestibility of rations containing increasing percentages of HSM in meat rabbits. Thirty-two Californian rabbits, assigned randomly to one of four treatments, were used in a generalized complete block design experiment with two 35-d feeding periods. Treatments replaced soybean meal and alfalfa such that 0%, 25%, 50%, and 75% CP was replaced by HSM. Diets were isonitrogenous and contained 28% steam-flaked corn. Weights were collected every 7 d, and total feed, orts, and fecal samples were collected in the final 7 d of feeding for digestibility determination. Rabbits were harvested after feeding periods, and stomach (S) and cecum (C) content sampling was conducted. Feed, orts, and fecal samples were assayed for DM and fiber fractions for digestibility determination. There was no effect of dietary treatment on DMD (52.5%; P = 0.05), NDFD (-29.5%; P = 0.54), or ADFD (-57.7%; P = 0.56). There was also no effect of dietary treatment on S NDF (11.9 g; P = 0.62), S ADF (8.4 g, P = 0.80), S ADL (2.6 g; P = 0.88), C NDF (8.0 g; P = 0.23), C ADF (5.5 g; P = 0.26), or C ADL content (3.2 g; P = 0.33). S and C digesta data were calculated as a ratio (S:C) to evaluate fiber disappearance in GI tract. However, there was no effect of treatment on S:C NDF (1.6:1; P = 0.43), S:C ADF (1.6:1; P = 0.42), or S:C ADL (1.4:1; P = 0.34). Despite lack of differences, ADG was greatest (P &lt; 0.05) from 25% HSM (35.1 g ± 1.16), least from 75% HSM (20.7 g ± 1.20), and intermediate from 0% (30.5 g ± 1.20) and 50% HSM (33.2 g ± 1.16). Data interpreted to mean that HSM may be a viable feedstuff in rabbit diets up to 50% without detrimental effects.
Laparoscopic surgery-associated massive subcutaneous emphysema requiring mechanical ventilation in a patient with endometriosis: a case report
Authors: Tsuyoshi Hideaki , Inoue Daisuke , Miyazaki Yumiko , Kawamura Hiroshi , Onuma Toshimichi , Kurokawa Tetsuji , Yoshida Yoshio 
Published: March 2022

Abstract
Although subcutaneous emphysema is a common benign complication of laparoscopic surgery, airway obstruction can occur due to pharyngeal emphysema when it extends to the neck. Here, we report a case of subcutaneous emphysema extending to the neck that required mechanical ventilation in a 51-year-old patient with endometriosis and severe adhesions during total laparoscopic hysterectomy and bilateral salpingo-oophorectomy. Although surgical or disease-specific risk stratification has not yet been established, the severe adhesions due to endometriosis and massive peritoneal defect due to the procedure might lead to the fragility of the subcutaneous tissue, resulting in a massive subcutaneous emphysema. This study highlights the importance of preoperative risk assessment in addition to intraoperative and postoperative monitoring for ventilation disorders and subcutaneous emphysema.
A Hybrid Covariate Microaggregation Approach for Privacy-Preserving Logistic Regression
Authors: Juwara Lamin , Saha-Chaudhuri Paramita 
Published: March 2022

Abstract
Distributed data networks (DDNs) with horizontally partitioned datasets are viable resources for multicenter research studies and pharmacosurveillance. Within DDNs, maintaining confidentiality and limiting the disclosure of sensitive information is critical. Consequently, data sharing between partners within the same network is either restricted or completely prohibited during statistical modeling. Current privacy-preserving methods for logistic regression span two extreme paradigms: meta-analysis (MA), which combines estimates based on partner-specific estimates, is convenient for the analytical center (AC) but requires separate implementations of the analysis by each data node; while distributed regression (DR), which provides overall estimates based on partner-specific data summaries, produces rigorous solutions but is an iterative process that is both time and resource consuming. A practical middle ground that combines the convenience of MA and the rigor of DR is lacking. We propose a likelihood-based approach for logistic regression modeling that combines the rigor of DR and the convenience of MA. The two-stage approach has an equivalent estimation performance as DR but foregoes its multiple iterative steps through an MA update step, and is therefore more user-friendly. The approach uses only aggregate-level covariates to estimate a starting pooled effect estimate and within-node data summaries for a single-shot update of the pooled estimate without requiring individual covariate values at the AC. We call the approach hybrid Pooled Logistic Regression (hPoLoR) and show that it conveniently provides accurate and efficient estimates of the standard individual-level log odds ratios and standard errors without revealing personal data. Hence hPoLoR provides a rigorous yet convenient and application-friendly alternative to MA and DR. The method is demonstrated through extensive simulations and application to the JCUSH data.
Large-Bore Mechanical Thrombectomy of Acute Pulmonary Embolism at a Community-Based Hospital: A Case Series
Authors: Ballas Elissa R , Sanders Christopher D , Hoskins Jason D 
Published: March 2022

ABSTRACT
Acute pulmonary embolism (PE) is a common, and sometimes fatal, diagnosis that results in over 11,000 deaths in hospitalized patients in the USA annually. In patients with high-risk or high-intermediate-risk PE and especially in whom thrombolytic therapy is contraindicated, mechanical thrombectomy can be an effective treatment option. This case series outlines three patients with PE who were successfully treated with large bore aspiration thrombectomy by Interventional Radiology at a community-based military treatment facility (MTF). Two patients had presented to the emergency department with acute PE and a third of patients with acute PE were transferred from an outside hospital specifically for mechanical thrombectomy due to a complication from systemic anticoagulation. The patients were categorized as good candidates for immediate large-bore aspiration thrombectomy, a recently added capability at the MTF. The patients showed immediate improvement post-procedure and required only one night admission for observation to the intensive care unit. Implementation of this new capability for patients with acute high-risk or high-intermediate-risk PE or with contraindications to thrombolysis provides an alternative treatment with immediate, life-saving capability.
NuSTAR discovery of the hard X-ray emission and a wide-band X-ray spectrum from the Pictor A western hotspot
Authors: Sunada Yuji , Morimoto Arisa , Tashiro Makoto S , Terada Yukikatsu , Katsuda Satoru , Sato Kosuke , Tateishi Dai , Sasaki Nobuaki 
Published: March 2022

Abstract
Utilizing Chandra, XMM-Newton, and NuSTAR, a wide-band X-ray spectrum from 0.2 to 20 keV is reported from the western hotspot of Pictor A. In particular, the X-ray emission is significantly detected in the 3 to 20 keV band at 30σ by NuSTAR. This is the first detection of hard X-rays with energies above 10 keV from a jet termination hotspot of active galactic nuclei. The hard X-ray spectrum is well described with a power-law model with a photon index of Γ = 1.8 ± 0.2, and the flux is obtained to be (4.5 ± 0.4) × 10−13 erg s−1 cm−2 in the 3 to 20 keV band. The obtained spectrum is smoothly connected with those soft X-ray spectra observed by Chandra and XMM-Newton. The wide-band spectrum shows a single power-law spectrum with a photon index of Γ = 2.07 ± 0.03, excluding any cut-off/break features. Assuming the X-rays to be synchrotron radiation of the electrons, the energy index of the electrons is estimated as p = 2Γ − 1 = 3.14 ± 0.06 from the wide-band spectrum. Given that the X-ray synchrotron-emitting electrons quickly lose their initial energies via synchrotron radiation, the energy index of electrons at acceleration sites is estimated as pacc = p − 1 = 2.14 ± 0.06. This is consistent with the prediction of the diffusive shock acceleration. Since the spectrum has no cut-off feature up to 20 keV, the maximum electron energy is estimated to be no less than 40 TeV.
A mitochondrial RNA processing protein mediates plant immunity to a broad spectrum of pathogens by modulating the mitochondrial oxidative burst
Authors: Yang Yang , Zhao Yan , Zhang Yingqi , Niu Lihua , Li Wanyue , Lu Wenqin , Li Jinfang , Schäfer Patrick , Meng Yuling , Shan Weixing 
Published: March 2022

Abstract
Mitochondrial function depends on the RNA processing of mitochondrial gene transcripts by nucleus-encoded proteins. This posttranscriptional processing involves the large group of nuclear-encoded pentatricopeptide repeat (PPR) proteins. Mitochondrial processes represent a crucial part in animal immunity, but whether mitochondria play similar roles in plants remains unclear. Here, we report the identification of RESISTANCE TO PHYTOPHTHORA PARASITICA 7 (AtRTP7), a P-type PPR protein, in Arabidopsis thaliana and its conserved function in immunity to diverse pathogens across distantly related plant species. RTP7 affects the levels of mitochondrial reactive oxygen species (mROS) by participating in RNA splicing of nad7, which encodes a critical subunit of the mitochondrial respiratory chain Complex I, the largest of the four major components of the mitochondrial oxidative phosphorylation system. The enhanced resistance of rtp7 plants to Phytophthora parasitica is dependent on an elevated mROS burst, but might be independent from the ROS burst associated with plasma membrane-localized NADPH oxidases. Our study reveals the immune function of RTP7 and the defective processing of Complex I subunits in rtp7 plants resulted in enhanced resistance to both biotrophic and necrotrophic pathogens without affecting overall plant development.
BLISTER promotes seed maturation and fatty acid biosynthesis by interacting with WRINKLED1 to regulate chromatin dynamics in Arabidopsis
Authors: Huang Ruihua , Liu Mengling , Gong Guanping , Wu Pingzhi , Bai Mei , Qin Hongting , Wang Guohe , Liao Huimei , Wang Xiaoxiu , Li Yanqun , Wu Hong , Wang Xiaojing , Yang Chengwei , Schubert Daniel , Zhang Shengchun 
Published: March 2022

AbstractWRINKLED1 (WRI1) is an important transcription factor that regulates seed oil biosynthesis. However, how WRI1 regulates gene expression during this process remains poorly understood. Here, we found that BLISTER (BLI) is expressed in maturing Arabidopsis thaliana seeds and acts as an interacting partner of WRI1. bli mutant seeds showed delayed maturation, a wrinkled seed phenotype, and reduced oil content, similar to the phenotypes of wri1. In contrast, BLI overexpression resulted in enlarged seeds and increased oil content. Gene expression and genetic analyses revealed that BLI plays a role in promoting the expression of WRI1 targets involved in fatty acid biosynthesis and regulates seed maturation together with WRI1. BLI is recruited by WRI1 to the AW boxes in the promoters of fatty acid biosynthesis genes. BLI shows a mutually exclusive interaction with the Polycomb-group protein CURLY LEAF (CLF) or the chromatin remodeling factor SWITCH/SUCROSE NONFERMENTING 3B (SWI3B), which facilitates gene expression by modifying nucleosomal occupancy and histone modifications. Together, these data suggest that BLI promotes the expression of fatty acid biosynthesis genes by interacting with WRI1 to regulate chromatin dynamics, leading to increased fatty acid production. These findings provide insights into the roles of the WRI1–BLI–CLF–SWI3B module in mediating seed maturation and gene expression.
Correlation Between Genetic Abnormalities in Induced Pluripotent Stem Cell-Derivatives and Abnormal Tissue Formation in Tumorigenicity Tests
Authors: Yamamoto Takako , Sato Yoji , Yasuda Satoshi , Shikamura Masayuki , Tamura Takashi , Takenaka Chiemi , Takasu Naoko , Nomura Masaki , Dohi Hiromi , Takahashi Masayo , Mandai Michiko , Kanemura Yonehiro , Nakamura Masaya , Okano Hideyuki , Kawamata Shin 
Published: March 2022

Abstract
Cell therapy using induced pluripotent stem cell (iPSC) derivatives may result in abnormal tissue generation because the cells undergo numerous cycles of mitosis before clinical application, potentially increasing the accumulation of genetic abnormalities. Therefore, genetic tests may predict abnormal tissue formation after transplantation. Here, we administered iPSC derivatives with or without single-nucleotide variants (SNVs) and deletions in cancer-related genes with various genomic copy number variant (CNV) profiles into immunodeficient mice and examined the relationships between mutations and abnormal tissue formation after transplantation. No positive correlations were found between the presence of SNVs/deletions and the formation of abnormal tissues; the overall predictivity was 29%. However, a copy number higher than 3 was correlated, with an overall predictivity of 86%. Furthermore, we found CNV hotspots at 14q32.33 and 17q12 loci. Thus, CNV analysis may predict abnormal tissue formation after transplantation of iPSC derivatives and reduce the number of tumorigenicity tests.
Autologous Stem Cells Transplantation for No-Option Angiitis-Induced Critical Limb Ischemia: Recurrence and New Lesion
Authors: Liu Hao , Fang Yuan , Pan Tianyue , Fang Gang , Liu Yifan , Jiang Xiaolang , Chen Bin , Gu Shiyang , Wei Zheng , Liu Peng , Fu Weiguo , Yang Jue , Dong Zhihui 
Published: March 2022

Abstract
Although satisfying outcomes have been demonstrated in terms of autologous stem cell transplantation in the treatment of angiitis-induced critical limb ischemia (AICLI), few studies have systematically reported the recurrence conditions. In the current study, we aimed to investigate recurrence conditions of a relatively large AICLI cohort in our center during a long-term follow-up period. From May 2009 to August 2020, 181 patients with AICLI received peripheral blood mononuclear cells (PBMNCs) or purified CD34+ cells (PCCs) transplantation. The main outcomes included recurrence and new lesions. Patient demographic data, ischemic limb characteristics, interventional characteristics, etc., were identified and analyzed. A logistic multivariable regression was performed to identify the independent risk factors for recurrence by a stepwise selection of variables. One hundred forty-eight patients were enrolled in this study. The mean follow-up period was 62.3 ± 37.4 months (range 12-144 months). The 5- and 10-year recurrence-free rates were 88.5% (95% confidence interval [CI] 3.1%-82.6%) and 71.7% (95% CI 7.6%-58.2%), respectively. The 5- and 10-year new lesion-free rates were 93.2% (95% CI 2.2%-89.0%) and 91.7% (95% CI 2.7%-86.6%), respectively. The finding of multiple limbs involved (OR 1.322 95% CI 1.123-12.549, P = .036) and ischemia relief period ≥5 months (OR 3.367 95% CI 1.112-10.192, P = .032) were demonstrated to be independent risk factors for recurrence in patients with AICLI who underwent cell transplantation. For patients with AICLI who responded to cell transplantation, the durability of this therapy was satisfactory, with 5- and 10-year recurrence-free rates of 88.5% and 71.7%, respectively. Multiple limbs involved at admission and ischemia relief period ≥5 months were demonstrated to be independent risk factors for recurrence after transplantation.
Risk assessment of perioperative neurocognitive disorders, where are we now?
Authors: Soehle Martin , Coburn Mark 
Published: March 2022

Purpose of review
Perioperative neurocognitive disorders (PNDs) are among the most frequent complications after surgery and are associated with considerable morbidity and mortality. We analysed the recent literature regarding risk assessment of PND.

Recent findings
Certain genetic variants of the cholinergic receptor muscarinic 2 and 4, as well as a marked degree of frailty but not the kind of anaesthesia (general or spinal) are associated with the risk to develop postoperative delirium (POD). Models predict POD with a discriminative power, for example, area under the receiver operating characteristics curve between 0.52 and 0.94.

Summary
Advanced age as well as preexisting cognitive, functional and sensory deficits remain to be the main risk factors for the development of PND. Therefore, aged patients should be routinely examined for both preexisting and new developing deficits, as recommended in international guidelines. Appropriate tests should have a high discrimination rate, be feasible to be administered by staff that do not require excessive training, and only take a short time to be practical for a busy outpatient clinic. Models to predict PND, should be validated appropriately (and externally if possible) and should not contain a too large number of predictors to prevent overfitting of models.