bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Diversity Analysis of Wild Population Based on SNP Developed from Transcriptome Sequencing of Spot-Fin Porcupine Fish (Diodon hystrix)
Authors: ma jun , jiang shoujia , cao liu , wang haihsan , zhuo wenqi , wang zhou , dang tingting , wang xueyi , chen pan , hou xingrong , you xinxin , huang hai 
Published: May 2022

Abstract
Diodon hystrix is widely distributed in the tropical and subtropical ocean. Due to delicious taste and unique body shape, the catch of this fish in the South China Sea has been increasing year by year. It is urgent to evaluate the population status of D. hystrix in the South China Sea. Molecular markers are often used to evaluate the population status, but it has been a lack of relevant molecular markers for D. hystrix. In this study, we utilized the Illumina sequencing platform to generate transcriptome data to identify SNPs in D. hystrix muscle tissue. A total of 7.2 Mb clean reads with 94.92% Q20 bases were obtained, and 4,179 putative SNPs distributed among 22,618 genes were identified and confirmed. Randomly selected SNPs were validated, and 19 true SNPs (39.58%) were screened. We used these true SNP loci to verify the genetic diversity and population structure of a wild population of D. hystrix. The results showed that 18 SNP loci were polymorphic. The average PIC, Ne, Ho and He were 0.3354, 1.7893, 0.4963 and 0.4391, respectively. 14 SNP loci were consistent with Hardy-Weinberg equilibrium (HWE), which revealed that the population was in a genetically stable state. The haplotype network concluded that the population may be composed of two different clades. In conclusion, the transcriptomic sequencing can quickly obtain the SNP loci as molecular markers which can be used for population genetic diversity and population structure analysis. It provides necessary help for the conservation biology and resource evaluation of D. hystrix.
Morphological characteristics of summer truffle (Tuber aestivum Vittad.) from Bosnia and Herzegovina
Authors: Ljubojević Srđan , Vasilišin Ladislav , Vučić Goran , Velemir Ana 
Published: May 2022

The aim of this paper is to present as accurately as possible the morphological characteristics of the summer truffle, found in Bosnia and Herzegovina, and to compare them with the characteristics of the previously described specimens in other countries and regions.
Well-developed fruiting bodies of summer truffles (Tuber aestivum Vittad.) were found in oak and beech forests on calcareous soils in Bosnia and Herzegovina with the help of trained dogs.
On the basis of a representative sample, composed of specimens, collected during three consecutive truffle seasons, the following were analyzed: shape, size and mass of ascocarps, structure of the harvest, gleba color, size and shape of ascospores.
It was found, that predominant geometric shape of their fruiting bodies is ellipsoid with an average mass of 44.3 g. Most acocarps weighed less than 20 g. As the weight of the pieces increases, their frequency decreases, so that ascocarps over 60 g participate in number with only 20 % and by weight with 47.2 % of the total yield. The length of spores ranges from 26.0 to 35.8 μm, with an average of 30.3 μm, while the width of spores varies from 17.2 to 26.1 μm, with an average of 21.3 μm. The Q-shape parameter takes values in a wide range from 1.16, which roughly corresponds to the globular shape, to 1.64 for the distinctly ellipsoidal shape.
In sum, the shape and size of the summer truffle ascocarps and spores native to Bosnia and Herzegovina fit the general picture of this taxon.
Influence of adding wild berry powders on the quality of pasta products
Authors: Samilyk Maryna , Demidova Evgenia , Bolgova Natalia , Kapitonenko Anatoly , Cherniavska Tetiana 
Published: May 2022

Pasta is a popular food item among consumers all over the world. They have high energy value, low cost and long shelf life. However, the biological value of these products is quite low. Adding components rich in dietary fiber, vitamins and minerals to their composition will significantly improve their nutritional value. The purpose of the article is to analyze the possibility of producing pasta with the addition of derivatives from the processing of wild berries Sambucus nigra, Viburnum opulus, Hippophae rhamnoides L., which have a rich nutrient composition and are available raw materials. The technology, developed in the work for processing berries by osmotic dehydration, allows the use of gentle drying modes, which contributes to the maximum preservation of their biological value. Sensory evaluation of pasta was carried out according to the Croatian Official Methods, moisture content of pasta was determined by drying to constant weight. Sensory analysis of pasta showed that the addition of powders does not lead to a deterioration in their quality. Their appearance, taste, smell, shape and elasticity are improved. The quality of the pasta samples, containing 5 % of the powder, was determined as “good” according to the results of the evaluation of the tasting group. And the samples with a powder content of 10 % have a "high" quality. The addition of powders has practically no effect on the drying conditions and moisture content of the finished products. With the addition of Viburnum opulus powders, the moisture content is reduced compared to controls. Humidity of all samples is within the permissible limits, which indicates their resistance to storage
Genetic testing of allelic variants of PIZ (GLU342Lys, RS28929474) and PIS (GLU264Val, RS17580) of SERPINA1 gene in children with bronchial asthma
Authors: Shymanska Ivanna , Trutiak Оlena , Lychkovska Оlena , Makukh Halyna , Akopyan Hayane 
Published: May 2022

According to world publications, mutations in the SERPINA1 gene may be a genetic risk factor for severe chronic obstructive pulmonary disease and, consequently, rapid progression of respiratory dysfunction. This disease leads to a decrease in the level of alpha-1-antitrypsin protein. It is inherited by autosomal recessive type, but there are registered cases of codominance. In the absence of treatment, diseases of the respiratory system become chronic and lead to disability in adulthood.
Early diagnosis of AAT deficiency is important to prevent complications and reduce mortality among people with this pathology. Due to these factors, genetic testing of SERPINA1 gene mutations in children with chronic lung diseases is appropriate to detect and prevent severe complications, associated with AATD.
The aim of this work is to improve the effectiveness of early diagnosis of AAT deficiency in children with bronchial asthma and recurrent obstructive bronchitis by identifying different genotypes and phenotypes of A1AT deficiency, studying their relationship with the clinical course of respiratory diseases in children
CYBERBULLYING’S FORMS AND IMPLICATIONS AMONG STUDENTS WITH PESANTREN BACKGROUND AND ACTIVE SOCIAL MEDIA USERS
Authors: Riyanti Erni Dewi , Raharjo Galih Cipto , Widafina Fadhillah Zahra 
Published: May 2022

As internet users in Indonesia increased by 11% from the previous year, Indonesians are getting more immersive with digital environment. In addition, the pandemic may be one of the drivers of the increase. The trend of using social media has a significant impact on people's social life, including the occurrence of bullying through online media, known as cyberbullying. The study aims to identify forms and impacts of cyberbullying among students of Faculty of Islamic Studies, Universitas Islam Indonesia with pesantren background and active social media users. With the descriptive qualitative method, the researchers applied purposive sampling in determining the respondents. The results of this study indicate that 84.6% of respondents know the definition of cyberbullying. 61.5% of respondents stated that they experienced at least 2 of 7 types of cyberbullying. The interview shows 3 forms of cyberbullying, such as, online harassments, flaming, and exclusion. The informants' reactions to cyberbullying are feeling annoyed, angry, hurt, disappointed, afraid, shock, overthinking, insecure, anxious, embarrassed, sad, concerned, retaliation to the perpetrators, and suicidal attempt. The informants' coping mechanisms are meeting a professional, remaining silent, suppressing their feelings, holding back their anger, blocking any communication access to the bully, and conducting activities to distract them from the occurred cyberbullying (e.g., playing games, journaling, relaxing, and sleeping). The interview also indicates negative and positive consequences of cyberbullying. Finally, the informants believes that social media ethics promoted via digital literacy campaign to young adults will be a solution which will significantly contribute to preventing cyberbullying.
Downscaling model for rainfall based on the influence of typhoon under climate change
Authors: Lin Shiu-Shin , Hu Yan-Li , Zhu Kai-Yang 
Published: May 2022

Abstract
The effects of typhoon rainfall cannot be easily investigated to downscale the GCMs (general circulation models) data, because typhoons are short-term highly nonlinear processes. In this study, to explore the impact on the typhoon rainfall for downscaling models, the monthly rainfalls are divided into typhoon rainfall and non-typhoon rainfall. The GA-RBFN downscaling models, integrating genetic algorithm to optimize a radial basis function neural network, were established to enquire into the future rainfall under the effect of typhoons in Taichung and Hualien, Taiwan. The GCM data in this study included MRI-CGCM3 and CSIRO-Mk3.0 from the 5th Assessment Report of the Intergovernmental Panel on Climate Change. The historical monthly rainfall of Taichung and Hualien stations are collected from the Central Weather Bureau, Taiwan, and the best track of typhoons from the Joint Typhoon Warning Center. Principal component analysis (PCA) and the stepwise regression procedure (SRP) were adopted, respectively, to select input variables from the GCM data. The future rainfall trends and uncertainties are evaluated by the best GA-RBFN model, which is selected with the highest performance by applying holistic information criteria. The simulated results show that the model with variables transformed by PCA performs well in forecasting non-typhoon rainfalls, while the model with variables transformed by SRP performs well in forecasting monthly total rainfalls. According to the three classifications of future rainfalls in wet and dry seasons, the mid- and long-term rainfall amount are mainly low to normal for Taichung and normal to high for Hualien. For the long-term rainfall assessment and the probability analysis on exceeding historical rainfalls, the long-term rainfall variability of the two stations is higher than the mid-term rainfall variability. It demonstrates that the future rainfall estimation has high uncertainty under the force of typhoons. Finally, the rainfall variability in the dry period is higher than that in the wet season, and the average probability of the future dry season rainfalls exceeding historical rainfall is high.
Angiotensin-converting Enzyme Inhibitors and Angiotensin Receptor
Blockers as Potential Therapeutic Options for Pancreatic Cancer
Authors: Asgharzadeh Fereshteh , Geraylow Kiarash Roustai , Khazaei Majid , Avan Amir , Nassiri Mohammadreza , Hassanian Seyed Mahdi , Ferns Gordon A 
Published: May 2022

Abstract:
The renin-angiotensin system (RAS) has been reported to have a role in carcinogenesis, and
therefore it may be of value as a potential therapeutic target in inhibiting tumor growth. It has been
shown that inhibition of RAS via angiotensin I-converting enzyme inhibitors (ACEIs) and angiotensin
II type-1 receptor (ARBs) inhibitors may have a protective effect against several malignancies. Here,
we provide an overview of the potential value of the RAS pathway and targeting via ACE/ARB inhibitors
in pancreatic cancer. Whilst the potential role of RAS as a target for the treatment of pancreatic
cancer has been reported, the use of candesartan with gemcitabine failed to improve outcomes in pancreatic
cancer. Another study of 1-3 years using ARB was found to reduce the risk of pancreatic cancer.
In line with these trials, others have demonstrated that the ARBs in combination with gemcitabine
might improve clinical outcomes in patients with advanced pancreatic cancer. Prospective trials are
warranted to investigate this hypothesis.
Oxytocin Effect in Adult Patients with Autism: An Updated Systematic
Review and Meta-Analysis of Randomized Controlled Trials
Authors: Kiani Zahra , Farkhondeh Tahereh , Aramjoo Hamed , Aschner Michael , Beydokhti Hossein , Esmaeili Aliakbar , Arab-Zozani Morteza , Samarghandian Saeed 
Published: May 2022

Introduction:
The efficacy of oxytocin in the treatment of autism spectrum disorder (ASD)
has not been fully characterized. This systematic review and meta-analysis study evaluated randomized
controlled trials (RCTs) on the treatment of intranasally administered oxytocin for autism.

Method:
The study was conducted in accordance with the PRISMA statement. Two authors searched
Scopus, PubMed/ Medline, Google Scholar, and Web of Science search engines and databases from
inception through December 2020. Quality assessment was carried out by with the “ROB-2, Cochrane
collaboration's tool”. The random-effects model was used for pooled analyses. I2 and Q tests were
used to investigate study heterogeneity. The visual inspection of funnel plots along with Egger’s regression
asymmetry test was used to assess the potential sources of publication bias.

Results:
Ten RCTs were selected for the systematic review. No study corroborated the efficacy of oxytocin
for the treatment of anxiety and repetitive behavior. One out of 4 studies reported clinical improvement
in severity, and 1 out of 6 studies indicated improvement in social function. Our metaanalyses
findings suggest that oxytocin shows no significant efficacy in the treatment of anxiety
(SMD: -0.168, SE= 0.112; 95% CI: -0.387, 0.050, p = 0.132), repetitive behavior (SMD: -0.078, SE=
0.155; 95% CI: -0.382, 0.225, p = 0.614), social function (SMD: -0.018, SE= 0.133; 95% CI: -0.279,
0.242, p = 0.891) and severity (SMD: -0.084, SE= 132; 95% CI: -0.343, 0.175, p = 0.524) of autism.
No significant heterogeneity nor publication bias were observed between studies.

Conclusions:
Our findings failed to corroborate the efficacy of oxytocin in the treatment of ASD.
Nonetheless, given the several limitations of our study, the results should be interpreted cautiously and
stimulate future research on this timely topic.
COMPUTER VISION SYNDROME–A PERSPECTIVE ON ASSESSMENT AND INTERVENTION
Authors: MADHURI JAMPANA , IKRAM SYEDA SADIYA , PURI SANJEEV KUMAR 
Published: May 2022

Objective: The aim of the study was to assess the common symptoms, and its associated factors and to identify its ocular morbidity among them due to prolonged use of digital devices.
Methods: A random sampling technique are used to calculate the sample size of 278. The questionnaire includes computer vision syndrome symptoms and factors associated with Computer Vision Syndrome development.
Results: A total of 278 health care students participated in this study. The mean age of health care students was 20.68 y±2.51 standard deviation and the range was between 18-25 y. 40.6% were males and 59.3% were females. Among digital devices, 85.9% of student’s use mobile phones frequently, 61.8% spent continuously on digital devices and had a higher risk of developing Computer Vision Syndrome, 40.2% Students were aware of 20-20-20 rule, 37% were aware of using digital devices at 40-75 cm, in pre-assessment. Thereby providing awareness and knowledge by showing them awareness video helps to improve the results in post-assessment.
Conclusion: There was a high prevalence of Computer Vision Syndrome symptoms among health care students in pre-assessment which was reduced in post-assessment. Thereby health care students who have knowledge on their eye care helps to prevent vision problems and it is also helpful for the people who are already having symptoms and ergonomic practices. So, they may consult the doctor without neglecting their visual problems.
SWARNA PRASHAN HAS A PRESSING NEED FOR TIME AMONG YOUNG ONES
Authors: CHATURVEDI CHAKRAPANI , VYAS PREM PRAKASH , CHATURVEDI SAKSHI 
Published: May 2022

Covid-19 calls for immunity boosters especially among children and the young generation. Ayurvedic preparations are another safer choice to build immunity naturally. Swarna Prashan is a safe ayurvedic combination that is beneficial for the normal growth and development of a child. Swarna Prashan is introducing Swarna (GOLD METAL) alone or along with herbs in the liquid form as an oral vaccine by children. Swarna Prashan is one of the samskaras in Ayurveda. Swarna Prashan is initiated from infancy till Adolescence. To review the components of swarnaprashna, clinical trials, and evidence of benefits for children. Ayurveda has deep roots for the Current and old method of introduction of Swarnaprashna among children below 16 y of age group in India. Overall results of all studies showed improved growth and development after the introduction of Swarna Prashan within a year without any side effects and a happy childhood which shows not only health but also wellbeing.
Hubungan Manajemen Sumber Daya Manusia Hijau dan Kinerja Berkelanjutan: Tinjauan Literatur
Authors: Muchtadin Muchtadin 
Published: May 2022

Masih minimnya penelitian empiris mengenai hubungan antara manajemen sumber daya manusia hijau dengan kinerja berkelanjutan. Dalam artikel ini, kami mencoba meninjau dan menganalisis penelitian yang relevan, kemudian kami mengusulkan agenda penelitian untuk memandu penelitian masa depan terkait topik ini. Penelitian ini menggunakan metode tinjauan pustaka. Sebanyak lima jurnal digunakan dalam penelitian ini dengan bahasa pengantar bahasa inggris dan terindeks googlescholar. Hasil penelitian menyimpulkan bahwa manajemen sumber daya manusia hijau mampu meningkatkan kinerja berkelanjutan juga mampu meningkatkan aspek-aspeknya seperti kinerja ekonomi, kinerja sosial, kinerja lingkungan. Penelitian di masa depan di harapkan dapat menggunakan variabel mediasi seperti environmental passion, komitmen organisasi karyawan, perilaku ramah lingkungan karyawan, pemberdaya budaya organisasi hijau, perilaku kewarganegaraan organisasi lingkungan, inovasi hijau, perilaku pro-lingkungan terkait tugas, perilaku pro-lingkungan proaktif, perilaku ramah lingkungan, modal intelektual hijau, perilaku pro-lingkungan. Selain itu dapat menggunakan metode kualitatif, campuran serta kuantitatif secara longitudinal.
Retracted: Quality of Life Following Major Limb Amputation in a Rural Community in Cameroon
Authors: Chunteng Nana Theophile , Yamben Ngo , Mertens Bombah F , Jeazet Wamba Y , Alex Manga , Clement Assob 
Published: May 2022

The following abstract has been retracted at the request of the Editors and the Publisher, due to duplicate publication, and full publication of the journal article will therefore not follow.
Adhering to the international guidelines established by the Committee on Publication Ethics, the Journal has determined the duplicate publication of this article is grounds for retraction. We regret the academic record was compromised and apologise to readers.
Abstract published e-pub ahead of print: 2022-05-18 in Journal of Rehabilitation Medicine (see version 1)

Background: Amputation is considered the last resort when a limb is no longer salvageable, a limb is dead or dying, is viable but non-functional, or is endangering the patient’s life. Limb amputation is associated with profound economic, social, and psychological effects on patients. The aim of this study is to evaluate quality of life following major limb amputation in a rural setting in west Cameroon.Methods: This was a cross-sectional descriptive, analytical study. Participants were interviewed and data collected using a pre-defined accredited questionnaire of the WHOQOL-BREF to assess quality of life.Results: There were 63 participants, and a majority (60.32% ) reported trauma as the cause of amputation. Participants with a prosthesis had better quality of life.Conclusion: Quality of life of people following major limb amputation in this study was generally fair according to the World Health Organization (WHO) quality of life tool.
Use of Computerized Microtomography, Energy Dispersive Spectroscopy, Scanning Electron Microscopy, and Atomic Force Microscopy to Monitor Effects of Adding Calcium to Bleaching Gels
Authors: Mendonça LC , Rodrigues MLA , Bicalho AA , daSilva GR , Quagliatto PS , Soares CJ 
Published: May 2022

SUMMARY

Objectives
The aim of this study was to evaluate the mineral content, expressed by calcium (Ca) and phosphate (P), in dental enamel exposed to bleaching agents using micro-computed tomography (micro-CT), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and atomic force microscopy (AFM).

Methods
Sixty bovine dental enamel specimens were randomly divided into three groups (n=20): HP35ca (bleached using 35% hydrogen peroxide with Ca); HP35wca (bleached using 35% hydrogen peroxide without Ca); and control (without bleaching). Five specimens from each group were used for SEM and EDS analyses, 10 specimens were used for AFM analysis, and the remaining five specimens were used for micro-CT analysis. The pH of the gels was measured using a pH meter. The EDS and micro-CT data were analyzed using one-way ANOVA and Pearson’s correlation test. The AFM data were analyzed using one-way ANOVA (α=0.05).

Results
The weight percentages of Ca and P obtained using EDS were similar between the bleached and control groups. Small, superficial changes were observed by SEM in the HP35wca group. The HP35ca group showed similar patterns to the control group. AFM results showed no significant changes in the enamel roughness in any of the tested groups. No significant difference in the volume or depth of structural enamel loss was found between gels with and without Ca. No mineral loss was observed in the dentin substrate. The EDS and micro-CT analysis data exhibited a high correlation (p&lt;0.001).

Conclusion
The addition of Ca to the bleaching gel had no beneficial effect on the bleached tooth enamel in terms of composition, mineral loss, and surface roughness. Micro-CT results exhibited a high correlation with the EDS results.
Exclusion of Human Right Protection Features in Populist Policy Making
Authors: Bavili Negin 
Published: May 2022

In this research, policies as output of policy making process are examined. Factors that have affected these policy making process are spotted. At first, different features which affec-ting policy making process is elaborated.Second, populism as a feature that affects policy ma-king is studied. Inputs, procedures that affect policy making and outputs are evaluated. Then How populist policy making is differentiated is clarified. This study will evaluate whether out-put policies have considered humanitarian features. More particularly, the focus of study will be on immigration policies in European count-ries like Denmark and UK. Whether protection of human right is considered in populist policy making context or ignored. This study will use qualitative methodology and will work on policies and the factors that have affected immigration policies. Also, how the populism have affected these process is perceived by analysis of real world output of these policies. Then the research will attempt to see populists with regard how populism affect immigration policies.