bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blunted rest-activity circadian rhythm increases the risk of all-cause, cardiovascular disease and cancer mortality in US adults: the NHANES 2011-2014 linked mortality study
Authors: Xu Yanyan , Su Shaoyong , Li Xinyue , Mansuri Asifhusen , McCall William V. , Wang Xiaoling 
Published: August 2022

Abstract
Background: Abnormality in rest-activity rhythm is now considered as a manifestation of long-term mild disruption of circadian rhythm in the daily real-world setting. However, whether blunted rest-activity circadian rhythm is associated with increased mortality risk in the general U.S. has not been assessed. Therefore, we aimed to examine whether rest-activity circadian rhythm parameters can predict all-cause, cardiovascular disease (CVD) and cancer mortality in a general adult population of the US. We further compared the mortality predictive performance of these parameters with that of traditional risk factors.Methods: This study included 7,252 adults from US National Health and Nutrition Examination Surveys (NHANES) 2011-2014, who had wrist accelerometer data obtained at baseline and follow-up status linked to the National Death Index records (2011-2019). Cox proportional hazards regression models were performed to estimate Hazard Ratios (HR) and 95% CIs, adjusted for sociodemographic factors, health status and sleep parameters obtained from the same set of accelerometer data. Predictive performance was assessed using 10-fold cross-validated concordance for Cox regression models.Results: Among the 7252 individuals (3826 female (53.4%); median [IQR] age, 49 [36-62] years) in the study, during a median of 81 months (interquartile range, 69-94 months) of follow-up, 674 (9.3%) deaths occurred. There were inverse associations between RA and all-cause mortality, CVD and cancer mortality with increased quartiles of RA associated with lower mortality risk (all P<0.05). The HR ranged from 0.61 to 0.79. Furthermore, RA outperformed all the tested traditional predictors of all-cause mortality with the exception of age.Conclusions: This study suggests that participants with blunted rest-activity circadian rhythms had a higher risk of all-cause, CVD and cancer mortality. Future studies will be needed to test whether interventions that regulate rest-activity circadian activity rhythms will improve health outcomes.
The Fire Under the Ash: Parasitic infections as potential risk factors for Attention Deficit Hyperactivity Disorder (ADHD) in children
Authors: Elmehy Dalia , Elmansory Basma M. , Gamea Ghada A. , Abdelhai Dina I. , Abd-Elsalam Sherief M. , Salamah Abeer M. , Ata Dina S. , Mahmoud Eman F. , Ibrahim Hoda A. , Salama Amina M. 
Published: August 2022

Abstract
Attention deficit hyperactivity disorder (ADHD) represents a mysterious neuropsychiatric alarming concern due to indefinite etiopathogenesis among children. Notably, the studies which investigated the correlation between ADHD and parasitic infections are insufficient. Therefore, this research aimed to assess the correlation between ADHD and some tissue dwelling and intestinal parasitic infections in children. The study was conducted on 200 children, including 100 children suffering from ADHD (Group I) and 100 healthy children as a control group (Group II). All caregivers fulfilled predesigned sociodemographic form and Conners parent rating scale (CPRS-48) questionnaire. Blood samples were collected to determine hemoglobin level as well as relative eosinophilic count. The presence of anti-Toxoplasma IgG and anti-Toxocara IgG in serum by Enzyme-Linked Immunosorbent Assay (ELISA) was further investigated. Also, micronutrients as zinc, iron, and copper levels were measured. Schistosoma antigen was investigated in urine samples. Stool samples were subjected to direct wet smear, concentration technique and modified Ziehl-Neelsen (MZN) staining for coccidian parasites detection. Cryptosporidium parvum, Giardia lamblia and Entamoeba histolytica antigens were investigated in stool samples. Group I expressed more liability to sociodemographic risk factors, decreased levels of Hb, iron, zinc, and copper with statistically significant difference (P < 0.001). Comparison between Group I and Group II regarding the detected parasitic infections exhibited statistically significant difference except Schistosoma antigen positivity which expressed no statistical significance. The present study concluded that the parasitic infections with their consequences are potential risk factors in children with ADHD indicating that their early diagnosis and treatment may help in ADHD prevention.
Components of Salvia miltiorrhiza and Panax notoginseng against OGD/R-treated injury in Pericytes via regulating PI3K/AKT and JNK/ERK/P38 signaling pathways
Authors: Zhang Tong , Liu Wenjie , Yang Juan , Xu Haiying , Sun Jin , Liang Bing , Cao Yushuang , Guo Lichen , Du Xinyuan , Chai Lijuan , Yuan Qing , Hu Limin 
Published: August 2022

Abstract
Salvia miltiorrhiza (SAL) and Panax notoginseng (PNS) is widely used for the treatment of ischemic stroke. However, what components of SAL and PNS protect brain microvascular pericytes after ischemic stroke remains unclear. We evaluated protective effects and mechanisms of the components of SAL and PNS in pericytes subjected to oxygen-glucose deprivation /reoxygenation (OGD/R). Pericytes were subjected to OGD/R. Cell Counting Kit-8 (CCK-8) was performed to evaluate cell viability. ROS and SOD kits were performed to detect oxidative stress. Flow cytometry was performed to analyze cells apoptosis. Scratch assay was performed to evaluate cells migration. Western blot was performed to detected expression of apoptosis proteins, VEGF, Ang-1, PDGFRβ, PI3K/AKT and JNK/ERK/P38 signaling pathways. Results showed Salvianolic acid B (Sal B), Salvianolic acid B (Sal D), Notoginsenoside R1(R1), Ginsenoside Rb1 (Rb1) and Ginsenoside Rg1 (Rg1) increased cell viability of pericytes subjected to OGD/R, reduced the level of ROS and increased the expression of SOD. The components reduced cells apoptosis, increased the proteins level of Bcl-2/Bax, reduced the level of Cleaved caspased3/caspase3, increased cells migration and enhanced the levels of Ang-1, PDGFR-β and VEGF. The components could activate PI3K/AKT/mTOR pathway, inhibit JNK/ERK/P38 pathway. Studies found that Sal B, Sal D, R1, Rb1 and Rg1 inhibited oxidative stress and apoptosis, increased release of pro-angiogenic regulators of pericytes-related to PI3K/AKT/mTOR and JNK/ERK/P38 signaling pathways. This provides a candidate basis for the development of monomeric drugs for treatment of ischemic stroke.
Association studies between chromosomal regions 1q21.3, 5q21.3, 14q21.2 and 17q21.31 and numbers of children in Poland
Authors: Clark Jeremy S.C. , de Wetering Thierry van , Marciniak Błażej , Żądzińska Elżbieta , Ciechanowicz Andrzej , Kaczmarczyk Mariusz , Boroń Agnieszka , Rydzewska Kamila , Posiadło Konrad , Strapagiel Dominik 
Published: August 2022

Abstract
Number of children is an important human trait: studies have indicated associations with single-nucleotide polymorphisms (SNPs). Aim: evaluate further evidence for four associations using a large sample of Polish subjects. POPULOUS genetic data provided from anonymous, healthy, unrelated, Polish volunteers of both sexes (N=5760). SNPs (n=173): (a) 69 from the chromosome 17 H1/H2 inversion; (b) six from 1q21.3, 5q21.3 and 14q21.2; and (c) 98 random negative controls. Zero-inflated negative-binomial regression (z.i.) was performed (0-3 children/individual (NCI) as non-events; adjustors: year of birth, sex). Significance level p=0.05 with Bonferroni correction. With both sexes combined, no positive results were obtained from negative-control SNPs or (b). Statistically-significant differences (combined or male data) were obtained from highly-linked inversion SNPs: representative rs12373123 gave combined mean NCI: TT: 2.31 (n=1418); CT: 2.35 (n=554); CC: 2.44 (n=43) (genotype p=0.01; TTvs.CC p=0.004; CTvs.CC p=0.009). Recessive modeling indicated H2-homozygotes had 0.118 more children than H1-homozygotes+heterozygotes (z.i.-count estimates+standard errors: CT, -0.508+0.194; TT, -0.557+0.191). The non-over-dispersed count model detected no interactions. Conclusions: association between the H1/H2 inversion and numbers of children (previously reported in Iceland) has been confirmed, albeit using a different statistical model. One limitation is the small amount of data, despite initially ~6000 subjects. Causal studies require further investigation.
“Factors predicting admission of psychiatric emergency contacts after presenting to the emergency department – results of a regression analysis.”
Authors: Kirchner Heribert , Schäfer Martin , Ullrich Heiko , Hulsmans Nik , Juckel Georg , Brzoska Patrick , Pajonk Frank Gerald 
Published: August 2022

Abstract
BackgroundPsychiatric emergency patients have great relevance in the interdisciplinary emergency department. Emergency physicians in this setting often have to make decisions under time pressure based on incomplete information regarding the patient's further treatment. The aim of this study was to identify possible predictors associated with an increased likelihood of inpatient psychiatric admission, thereby facilitating future decision-making processes for clinicians regarding further treatment.MethodsA retrospective cross-sectional study of all psychiatric emergency contacts in an interdisciplinary emergency department (ED) of a general hospital in a large German city was conducted for 2015. A binary regression analysis was performed to identify possible predictors.ResultsFactors associated with an increased probability of inpatient psychiatric admission were previously known patients, patients under a legal care order (guardianship), and previous outpatient medical contact. No association for gender or age was found. Data demonstrated a negative relationship between a neurotic, stress-related and somatoform disorder diagnosis and admission.ConclusionsThe conducted study showed some significant characteristics associated with an increase likelihood of emergency admission. Particularly noteworthy is the negative relationship between a neurotic, stress-related and somatoform disorder diagnosis and admission. To improve the treatment of patients in emergency units, these factors should be taken into account. Further studies should be undertaken in order to develop specific emergency admission algorithms comparable to internal medicine.
Effectiveness of low-dose radiation therapy to improve mortality in coronavirus disease (COVID-19): A Systematic Review and Meta-Analysis
Authors: Sayfollahi Sahar , Momeni Zeinab , Bagherzadeh Sakineh , Goli-Ahmadabad Foad , Banaei Amin , Rashidi Mehdi , Bijari Salar 
Published: August 2022

Abstract
Background
Before antibiotics were available, X-ray therapy was used to treat pneumonia, but its effectiveness is unknown. It was not fully understood how low-dose radiation could reduce inflammation in early attempts to treat pneumonia with X-rays. Exposure to low-dose radiation therapy (LDRT) may activate anti-inflammatory molecules such as TGF-b1 and IL-10. This systematic review and meta-analysis aimed to determine whether LDRT effectively treats COVID-19-associated pneumonia.
Methods
This study followed the PRISMA guidelines (Principles for Reporting Systematic Reviews and Meta-Analyses). A search was conducted on Google Scholar and PubMed for articles published before June 12, 2022. The Newcastle–Ottawa Scale (NOS) was used to evaluate the included research quality.
Results
In these studies, patients were given lung radiation at doses ranging from 0.5 to 1.5 Gy. Except for one study, all clinical, radiological, and survival outcomes improved. The study showed that whole-lung radiation at doses of 0.5–1.5 Gy was beneficial to patients with COVID-19-associated pneumonia who were oxygen-dependent. In these studies, the reported SR was 59% (95% CI, 44–75%).
Conclusions
LDRT has the potential to be a viable therapy option, although large-scale randomized trials are necessary, particularly in the elderly, who are more at risk of mortality from COVID-19.
Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma
Authors: Kong Fanhua , Wang Kunpeng 
Published: August 2022

AbstractBackground Hepatocellular carcinoma (HCC) is one of the major contributors to cancer burden worldwide, and its incidence has increased in recent decades. N6-methyladenosine (m6A) modification plays an important role in the tumorigenesis and metastasis of HCC by changing gene expression and function at multiple levels, such as ribonucleic acid (RNA) splicing, stability, translocation and translation. Methods The ONCOMINE, UALCAN, GEPIA, Kaplan–Meier plotter, cBioPortal for Cancer Genomics, STRING and TIMER2 databases were used for bioinformatic analyses. Quantitative polymerase chain reaction and western blotting were used to detect the expression of m6A RNA methylation regulators in HCC tissues. Results The transcription of m6A RNA methylation regulators was upregulated in patients with HCC, and overexpression of YTHDF1/2, YTHDC1, RBM15 and METTL3 was significantly correlated with clinical stages of HCC. In addition, downregulation of ZC3H13 and METTL14 and upregulation of other m6A RNA methylation regulators were associated with a poor prognosis. A high mutation rate (89%) of m6A RNA methylation regulators was also observed in patients with HCC, and mutations in methylation regulators were associated with poor overall survival and disease-free survival. Finally, the expression of the YTHDF family was significantly associated with immune infiltration in the HCC microenvironment. Conclusion m6A RNA methylation regulators and programmed death-ligand 1 may play an important role in the tumorigenesis and immune invasion and escape of HCC and may be risk factors affecting the survival of patients with HCC.
Assessment of mineral composition and health status of five honey samples from southern Nigeria: drifting towards food quality control
Authors: OBIGBA SYLVESTER ONORIODE , EDEMA Noyo E. , JOHN Annette E. , ENEBELI Blessing N. 
Published: August 2022

Abstract

Mineral composition, interaction and safety index of five honey samples from southern Nigeria was investigated. Agilent 720 ICP-OES was used for the determination of mineral element concentrations (mg/l). Honey sample from Akure was highest in concentration (570.06 mg/l) while Ijala-Ikeren was lowest (90.25 mg/l). The mean and ranges for minerals were: Akure; 27.15 (0.35–277.14), Ogunmakin; 13.13 (0.26–91.81), Ibadan; 9.05 (0.42–114.53), Agbor; 5.50 (0.01–58.02) and Ijala-Ikeren; 4.11 (0.14–39.81). The coefficient of variance ranged from 12.48–180.27% revealing variations in concentration. The most abundant mineral elements were Ca, 116.26 (39.82–277.14); Mg, 41.05 (3.43–173.05); Na, 33.67 (11.09–70.59); K, 30.08 (11.43–75.14); Zn, 4.60 (0.58–14.04); Al, 3.89 (1.05–8.03); U, 3.17 (2.11–4.53) and Fe, 2.85 (1.88–3.52). Heavy metals like Fe, Ba, Pb, Cr, Ni, Ag, As, Cu, Mn and Cd were above maximum permissible limits. The mineral ratio for Zn/Cu, Fe/Cu, Fe/Pb, and Zn/Cd indicated possible unhealthy interaction. The K/[Ca + Mg] values revealed potential hypomagnesaemic effect if continually consumed. The safety indices were all within recommended range except for Se. The principal component plot showed no particular mineral distribution pattern. Thallium had the highest hazard quotient (2.00–50.00) while the hazard indices were between 3.24 and 53.97 showing potential non-carcinogenic effect. The presence of essential trace and major minerals revealed potential usefulness of the honeys as food supplement; however, the heavy metals presence resonate the need for quality control, food safety and health risk assessment before open marketing.
Coronary microvascular dysfunction affects left ventricular global longitudinal strain response to dipyridamole stress echocardiography: a pilot study
Authors: Tagliamonte Ercole , Sperlongano Simona , Montuori Caterina , Riegler Lucia , Scarafile Raffaella , Carbone Andreina , Forni Alberto , Radmilovic Juri , Vilio Alessandro , Astarita Roberta , Cice Gennaro , D’Andrea Antonello 
Published: August 2022

Abstract
Aim: to investigate, by means of speckle tracking echocardiography, left ventricular (LV) contractile function at rest and during dipyridamole stress in patients with coronary microvascular dysfunction (CMD). Methods and Results: 59 patients (39% women, mean age 65.6 ± 6.1 years) with history of chest pain and without obstructive coronary artery disease (CAD) underwent dipyridamole stress echocardiography. Coronary flow was assessed in the left anterior descending coronary artery. Coronary flow reserve (CFR) was determined as the ratio of hyperaemic to baseline diastolic coronary flow velocity. CMD was defined as CFR < 2. Global longitudinal strain (GLS) was measured at rest and at peak dose. Nineteen patients (32%) among the overall population showed CMD. Baseline GLS was significantly lower in patients with CMD (-16.8 ± 2.7 vs. -19.1 ± 3.1, p < 0.01). A different contractile response to dipyridamole infusion was observed between the two groups: GLS significantly increased up to peak dose in patients without CMD (from -19.1 ± 3.1 to -20.2 ± 3.1, p < 0.01), and significantly decreased in patients with CMD (from -16.8 ± 2.7 to -15.8 ± 2.7, p < 0.01). There was a significant inverse correlation between CFR and DGLS (r = -0.82, p < 0.01).Conclusion: rest GLS and GLS response to dipyridamole stress are markedly impaired among patients with chest pain syndrome, non-obstructive CAD and CMD, reflecting subclinical LV systolic dysfunction and lack of LV contractile reserve due to underlying myocardial ischemia.
Differential effects of healthcare worker burnout on psychotropic medication use and misuse by occupational level
Authors: Hoopsick Rachel , Las Sylvia , Sun Rachel 
Published: August 2022

Abstract
Purpose
Burnout has been well-examined among physicians and other high-wage, high-autonomy healthcare positions. However, lower-wage healthcare workers with less workplace autonomy (e.g., medical assistants, nurses’ aides) represent a substantial proportion of the workforce but remain understudied. We aimed to examine the effects of burnout on psychotropic medication use and misuse and whether these effects differed by occupational level.
Methods
In March 2022, we collected data from a diverse sample of US healthcare workers (N = 200) and examined the cross-sectional relationship between burnout and changes in prescribed psychotropic medication (i.e., starting, stopping, and/or having a change in the dose/frequency) during the COVID-19 pandemic. We also separately examined the relationship between burnout and psychotropic medication misuse (i.e., without a prescription, in greater amounts, more often, longer than prescribed, and/or for a reason other than prescribed). We stratified models by occupational level (prescribers/healthcare administrators vs. other healthcare workers).
Results
Greater burnout was associated with higher odds of changes in prescribed psychotropic medication among physicians/administrators (aOR = 1.23, 95% CI: 1.01, 1.48), but not among other healthcare workers (aOR = 1.04, 95% CI: 0.98, 1.10). Greater burnout was not associated with psychotropic medication misuse among physicians/administrators (aOR = 0.96, 95% CI: 0.82, 1.12) but was associated with increased odds of psychotropic medication misuse among other healthcare workers (aOR = 1.07, 95% CI: 1.01, 1.14).
Conclusions
Potential disparities in help-seeking and healthcare access might manifest in non-medical use of prescription drugs among some healthcare workers, which has implications for worker safety and well-being.
Down-regulation of PEAR1 and its regulatory factor, LINC00271, as the potential novel tumor suppressors in breast cancer: bioinformatics and experimental approach
Authors: Sedighi Katayoun , Eskandari Fatemeh , Boushehri Parisa Ghanizade , Abari Sara Kiani , Saharkhiz Shaghayegh , Azadeh Mansoureh 
Published: August 2022

Abstract
In addition to coding region, non-coding genes can also be involved in the development of cancer cells. Therefore, the present study aimed to study the expression level of the coding gene PEAR1 and non-coding gene LINC00271 in breast tumors. Using bioinformatics approaches, the DE genes and miRNAs in breast cancer tissue were extracted from datasets. MiRNA-target regulatory network visualization in Cytoscape along with applying Cytohubba lead to identifying hub-miRNAs and their targets in breast tumors. Functional enrichment analysis was done using MIEAA database. The co_lncRNA and lncBASE databases were utilized to find effective lncRNA. Verifying the PEAR1 and LINC00271 expression in breast tumor and adjacent healthy cells was carried by Real-Time PCR assay. Analysis of array-based non-coding expression profile resulted in 96 significant DE miRNAs which 15 out of them were collected as hub-miRNAs due to the number of their target genes. 4 common genes were identified between targets of hub-miRNAs and DE genes in breast tumors; among them, PEAR1 was considered as novel DE gene which also was one of the targets of upregulated hub-miRNAs, hsa-miR-34a-5p. LINC00271 was selected as correlated lncRNA with PEAR1 and hsa-miR-34a-5p. qRT-PCR results revealed that the expression level of both PEAR1 and LINC00271 were significantly down-regulated (Fold change PEAR1 = 0.00798/P-Value < 0.0001, Fold change LINC00271 = 0.09/P-Value = 0.0094) in breast tumors compared to controls. Due to a significant reduction in PEAR1 and LINC00271 expression and array-based reported, the association between these genetic factors can be considered as new molecular markers in the prognosis and metastasis of breast cancer.
Intracranial Investigation of Piriform Cortex Epilepsy During Odor Presentation
Authors: Bearden Donald J. , Selawski Robyn , Chern Joshua J. , Valle Eva , Bhalla Sonam , Al-Ramadhani Ruba , Ono Kim E. , Pedersen Nigel P. , Zhang Guojun , Drane Daniel L. , Kheder Ammar 
Published: August 2022

Abstract
The piriform cortex is part of the olfactory system, principally receiving input from the lateral olfactory tract and projecting to downstream components of the olfactory network, including the amygdala. Based on preclinical studies, the piriform cortex is vulnerable to injury and can be easily kindled as an onset site for seizures. While the role of the piriform cortex in human epilepsy has been studied indirectly and the subject of speculation, cases of demonstrated piriform cortex seizure onset from direct intracranial recording are rare. We present a pediatric patient with drug-resistant focal reflex epilepsy and right mesial temporal sclerosis with habitual seizures triggered by coconut aroma. The patient underwent stereoelectroencephalography with implantation of olfactory cortices including the piriform cortex. We examined the field potential effects of odorant presentation, the location of seizure onset and ability to recapitulate habitual seizures from olfactory cortices. Surgical workup resulted in resection of the patient’s right amygdala, piriform cortex, and mesial temporal pole, following which she has been seizure free for 20 months without functional decline in cognition or smell.