bluuebunny/crossref_metadata_2025_split huggingface dataset:
High efficiency sorting and outgrowth for single-cell cloning of mammalian cell lines
Authors: Munoz Adonary , Morachis José M. 
Published: September 2022

AbstractSingle-cell selection and cloning is required for multiple bioprocessing and cell engineering workflows. Dispensing efficiency and outgrowth were optimized for multiple common suspension (CHO ES, Expi293F, and Jurkat) and adherent (MCF-7, A549, CHO-K1, and HEK293) cell lines. Single-cell sorting using a low pressure microfluidic cell sorter, the WOLF Cell Sorter, was compared with limiting dilution at 0.5 cells/well to demonstrate the increased efficiency of using flow cytometry selection of cells. In this work, there was an average single cell deposition on Day 0 of 89.1% across all the cell lines tested compared to 41.2% when using limiting dilution. After growth for 14 days, 66.7% of single-cell clones sorted with the WOLF Cell Sorter survived and only 23.8% when using limiting dilution. Using the WOLF Cell Sorter for cell line development results in higher viable single-cell colonies and the ability to select subpopulations of single-cells using multiple parameters.
Obesity and cancer—extracellular matrix, angiogenesis, and adrenergic signaling as unusual suspects linking the two diseases
Authors: Pellegata Natalia S. , Berriel Diaz Mauricio , Rohm Maria , Herzig Stephan 
Published: September 2022

AbstractObesity is an established risk factor for several human cancers. Given the association between excess body weight and cancer, the increasing rates of obesity worldwide are worrisome. A variety of obesity-related factors has been implicated in cancer initiation, progression, and response to therapy. These factors include circulating nutritional factors, hormones, and cytokines, causing hyperinsulinemia, inflammation, and adipose tissue dysfunction. The impact of these conditions on cancer development and progression has been the focus of extensive literature. In this review, we concentrate on processes that can link obesity and cancer, and which provide a novel perspective: extracellular matrix remodeling, angiogenesis, and adrenergic signaling. We describe molecular mechanisms involved in these processes, which represent putative targets for intervention. Liver, pancreas, and breast cancers were chosen as exemplary disease models. In view of the expanding epidemic of obesity, a better understanding of the tumorigenic process in obese individuals might lead to more effective treatments and preventive measures.
Evaluation of the rhizospheric microbiome of the native colonizer Piptatherum miliaceum in semiarid mine tailings
Authors: Conesa Héctor M. , Párraga-Aguado Isabel , Jiménez-Cárceles Francisco J. , Risueño Yolanda 
Published: September 2022

AbstractThe study of the rhizospheric microbiome in native plants should be a prerequisite before carrying out the phytomanagement of mine tailings. The goal of this work was to evaluate the rhizospheric microbiome of Piptatherum miliaceum in semiarid mine tailings. A comprehensive edaphic characterization was performed including the description of soil microbial composition in the rhizosphere of P. miliaceum growing at a mine tailings pile and at a control site. Plant nutritional and isotopic compositions were also determined. Neutral pH of the tailings (7.3) determined low metal extractability in 0.01 M CaCl2 (e.g. < 1 mg/kg for Zn). In spite of the contrasting edaphic fertility conditions of both sites, N (~ 15 g kg−1) and P (~ 400 mg kg−1) leaf concentrations were similar. The lower δ15N at the tailings plants (− 4.50‰) compared to the control (6.42‰) indicated greater efficiency of P. miliaceum for uptaking N under the low fertility conditions of the tailings (0.1% total soil nitrogen). The presence at the tailings of bacterial orders related to the cycling of N, such as Rhizobiales, could have contributed to enhance N acquisition. The lower leaf δ13C values at the tailings (− 30.22‰) compared to the control (− 28.47‰) indicated lower water use efficiency of the tailing plants. Some organotrophic bacterial and fungal groups in the tailings’ rhizospheres were also found in the control site (e.g. Cytophagales, Sphingobacteriales for bacteria; Hypocreales, Pleosporales for fungi). This may indicate that P. miliaceum is able to shape its own specific microbiome at the tailings independently from the initial microbial composition of the tailings.
Adversarial vulnerability bounds for Gaussian process classification
Authors: Smith Michael Thomas , Grosse Kathrin , Backes Michael , Álvarez Mauricio A. 
Published: September 2022

AbstractProtecting ML classifiers from adversarial examples is crucial. We propose that the main threat is an attacker perturbing a confidently classified input to produce a confident misclassification. We consider in this paper the $$L_0$$

L
0

attack in which a small number of inputs can be perturbed by the attacker at test-time. To quantify the risk of this form of attack we have devised a formal guarantee in the form of an adversarial bound (AB) for a binary, Gaussian process classifier using the EQ kernel. This bound holds for the entire input domain, bounding the potential of any future adversarial attack to cause a confident misclassification. We explore how to extend to other kernels and investigate how to maximise the bound by altering the classifier (for example by using sparse approximations). We test the bound using a variety of datasets and show that it produces relevant and practical bounds for many of them.
Numerical quadrature for singular integrals on fractals
Authors: Gibbs Andrew , Hewett David , Moiola Andrea 
Published: September 2022

AbstractWe present and analyse numerical quadrature rules for evaluating regular and singular integrals on self-similar fractal sets. The integration domain $$\Gamma \subset \mathbb {R}^n$$

Γ
⊂

R

n

is assumed to be the compact attractor of an iterated function system of contracting similarities satisfying the open set condition. Integration is with respect to any “invariant” (also known as “balanced” or “self-similar”) measure supported on $$\Gamma$$
Γ
, including in particular the Hausdorff measure $$\mathcal {H}^d$$

H

d

restricted to $$\Gamma$$
Γ
, where d is the Hausdorff dimension of $$\Gamma$$
Γ
. Both single and double integrals are considered. Our focus is on composite quadrature rules in which integrals over $$\Gamma$$
Γ
are decomposed into sums of integrals over suitable partitions of $$\Gamma$$
Γ
into self-similar subsets. For certain singular integrands of logarithmic or algebraic type, we show how in the context of such a partitioning the invariance property of the measure can be exploited to express the singular integral exactly in terms of regular integrals. For the evaluation of these regular integrals, we adopt a composite barycentre rule, which for sufficiently regular integrands exhibits second-order convergence with respect to the maximum diameter of the subsets. As an application we show how this approach, combined with a singularity-subtraction technique, can be used to accurately evaluate the singular double integrals that arise in Hausdorff-measure Galerkin boundary element methods for acoustic wave scattering by fractal screens.
Extracting Hale Cycle Related Components from Cosmic-Ray Data Using Principal Component Analysis
Authors: Takalo Jouni 
Published: September 2022

AbstractWe decompose the monthly cosmic-ray data, using several neutron-monitor count rates, of Cycles 19 – 24 with principal component analysis (PCA). Using different cycle limits, we show that the first and second PC of cosmic-ray (CR) data explain 77 – 79% and 13 – 15% of the total variation of the Oulu CR Cycles 20 – 24 (C20 – C24), 73 – 77% and 13 – 17% of the variation of Hermanus C20 – C24, and 74 – 78% and 17 – 21% of the Climax C19 – C22, respectively. The PC1 time series of the CR Cycles 19 – 24 has only one peak in its power spectrum at the period 10.95 years, which is the average solar-cycle period for SC19 – SC24. The PC2 time series of the same cycles has a clear peak at period 21.90 (Hale cycle) and another peak at one third of that period with no peak at the solar-cycle period. We show that the PC2 of the CR is essential in explaining the differences in the intensities of the even and odd cycles of the CR. The odd cycles have a positive phase in the first half and a negative phase in the second half of their PC2. This leads to a slow decrease in intensity at the beginning of the cycle and a flat minimum for the odd cycles. On the contrary, for the even cycles the phases are reversed, and this leads to faster decrease and more rapid recovery of the CR intensity of the cycle. As a consequence, the even cycles have a more peak-like structure. These results are confirmed with skewness–kurtosis (S–K) analysis. Furthermore, S–K shows that other even and odd cycles, except Cycle 21, are on the regression line with a correlation coefficient 0.85. The Cycles 21 of all eight stations are compactly located in the S–K coordinate system and have smaller skewnesses and higher kurtoses than the odd Cycles 23.
Cerebrovascular Gi Proteins Protect Against Brain Hypoperfusion and Collateral Failure in Cerebral Ischemia
Authors: Castaneda-Vega Salvador , Beer-Hammer Sandra , Leiss Veronika , Napieczyńska Hanna , Vuozzo Marta , Schmid Andreas M. , Zeng Hang , He Yi , Kohlhofer Ursula , Gonzalez-Menendez Irene , Quintanilla-Martinez Leticia , Hempel Johann-Martin , Gollasch Maik , Yu Xin , Pichler Bernd J. , Nürnberg Bernd 
Published: September 2022

Abstract
Cerebral hypoperfusion and vascular dysfunction are closely related to common risk factors for ischemic stroke such as hypertension, dyslipidemia, diabetes, and smoking. The role of inhibitory G protein-dependent receptor (GiPCR) signaling in regulating cerebrovascular functions remains largely elusive. We examined the importance of GiPCR signaling in cerebral blood flow (CBF) and its stability after sudden interruption using various in vivo high-resolution magnetic resonance imaging techniques. To this end, we induced a functional knockout of GiPCR signaling in the brain vasculature by injection of pertussis toxin (PTX). Our results show that PTX induced global brain hypoperfusion and microvascular collapse. When PTX-pretreated animals underwent transient unilateral occlusion of one common carotid artery, CBF was disrupted in the ipsilateral hemisphere resulting in the collapse of the cortically penetrating microvessels. In addition, pronounced stroke features in the affected brain regions appeared in both MRI and histological examination. Our findings suggest an impact of cerebrovascular GiPCR signaling in the maintenance of CBF, which may be useful for novel pharmacotherapeutic approaches to prevent and treat cerebrovascular dysfunction and stroke.
SHP2 regulates adipose maintenance and adipocyte-pancreatic cancer cell crosstalk via PDHA1
Authors: Olou Appolinaire A. , Ambrose Joe , Jack Jarrid L. , Walsh McKinnon , Ruckert Mariana T. , Eades Austin E. , Bye Bailey A. , Dandawate Prasad , VanSaun Michael N. 
Published: September 2022

AbstractAdipocytes are the most abundant cell type in the adipose tissue, and their dysfunction is a significant driver of obesity-related pathologies, such as cancer. The mechanisms that (1) drive the maintenance and secretory activity of adipocytes and (2) mediate the cancer cellular response to the adipocyte-derived factors are not fully understood. To address that gap of knowledge, we investigated how alterations in Src homology region 2-containing protein (SHP2) activity affect adipocyte function and tumor crosstalk. We found that phospho-SHP2 levels are elevated in adipose tissue of obese mice, obese patients, and differentiating adipocytes. Immunofluorescence and immunoprecipitation analyses as well as in-silico protein–protein interaction modeling demonstrated that SHP2 associates with PDHA1, and that a positive association promotes a reactive oxygen species (ROS)-driven adipogenic program. Accordingly, this SHP2-PDHA1-ROS regulatory axis was crucial for adipocyte maintenance and secretion of interleukin-6 (IL-6), a key cancer-promoting cytokine. Mature adipocytes treated with an inhibitor for SHP2, PDHA1, or ROS exhibited an increased level of pro-lipolytic and thermogenic proteins, corresponding to an increased glycerol release, but a suppression of secreted IL-6. A functional analysis of adipocyte-cancer cell crosstalk demonstrated a decreased migration, invasion, and a slight suppression of cell cycling, corresponding to a reduced growth of pancreatic cancer cells exposed to conditioned media (CM) from mature adipocytes previously treated with inhibitors for SHP2/PDHA1/ROS. Importantly, PDAC cell growth stimulation in response to adipocyte CM correlated with PDHA1 induction but was suppressed by a PDHA1 inhibitor. The data point to a novel role for (1) SHP2-PDHA1-ROS in adipocyte maintenance and secretory activity and (2) PDHA1 as a regulator of the pancreatic cancer cells response to adipocyte-derived factors.
Effectiveness of Patient Education and Cognitive Behavioural Treatment as a Non-Pharmacological Intervention for Migraine in Adults – a Systematic Review
Authors: Meise Ruth , Schwarz Annika , Luedtke Kerstin 
Published: September 2022

AbstractTo evaluate the content of patient education and cognitive behavioural therapy and its effectiveness in the prevention of migraine for adults, a systematic search was conducted in the databases MEDLINE, EMBASE, PsycINFO and CINAHL. RCTs published in the past 10 years in German or English, reporting on any form of patient education or cognitive behavioural therapy for adult migraineurs, were included. Two reviewers independently searched and evaluated search results. The methodological quality of selected studies was assessed using the Cochrane risk of bias tool 2.0 independently by two reviewers. Data on content and effectiveness were extracted in a predesigned table. Across 1059 records, 14 studies were eligible, including 2266 participants (82,7% females). Contents of patient education included explanations on the link between thoughts and feelings, information on lifestyle influences on headaches, relaxation techniques and stress management, advice on diet and the benefit of physical activity. In addition, patient education focussed on the pathogenesis and diagnostic criteria of migraine, triggering factors and effective acute or prophylactic drug use. Outcome measures included headache frequency, medication intake, function and disability, as well as psychosocial status. Education reduced migraine frequency and improved quality of life. Effect sizes were small, and contents were heterogeneous. Educational and behavioural approaches can positively influence headache frequency. However, the content, duration and frequency and education formats varied widely. The level of evidence for education and cognitive behavioural therapy as a non-pharmacological intervention for migraine is still low. The protocol for the systematic review was registered in the database PROSPERO (PROSPERO 2019 CRD42019134463) prior to the data collection.
Paul's Gospel of Divine Self-Sacrifice
Authors: Moser Paul 
Published: September 2022

In this book, Paul Moser explains how self-sacrificial righteousness of a reparative kind is at the heart of Paul's gospel of God. He also shows how divine self-sacrifice authenticates that gospel via human reciprocity toward God in reconciliation. A basis for this reciprocity lies in a teaching of ancient Judaism that humans are to reciprocate toward God for the sake of an interpersonal relationship that is righteous and reconciled through voluntary self-sacrifice to God. Moser demonstrates that Paul's gospel calls for faith, including trust, in God as reciprocity in human self-sacrifice toward God. Although widely neglected by interpreters, this theme brings moral and evidential depth to Paul's good news of reparative redemption from God. Moser's study thus enables a new understanding of some of the controversial matters regarding Paul's message in a way that highlights the coherence and profundity of his message.
The Wyman Fragment: A New Edition and Analysis with Radiocarbon Dating
Authors: Stevens Daniel 
Published: September 2022

AbstractThe Wyman Fragment, or 0220, has been a chief and early witness to the text of Romans 4.23–5.3 since the initial publication of the recto in 1952. A 2005 edition of the verso, containing Rom 5.8–13, rendered most of that portion of the manuscript legible for the first time, but has not been widely circulated. In this article I present a new edition and analysis of 0220, a third- or fourth-century manuscript of Rom 4.23–5.13, in light of multispectral imaging and radiocarbon testing performed on the fragment. This edition resolves tensions between previous editions, contextualises the radiocarbon and palaeographic dates, transcribes previously illegible traces, securing additional lines of text, and reveals more work by a second hand than previously noted.
The Reception of Pauline Mysticism: An Ideological Critique
Authors: Tofighi Fatima 
Published: September 2022

AbstractPaul was a mystic. So claimed scholars from Adolf Deissmann to Albert Schweitzer. Others disagreed, figures no less significant than Rudolf Bultmann, Karl Barth and Ernst Käsemann. The pro-mystic group argued that Paul's theological message was best understood if set within the context of Hellenistic or Jewish mysticism. The anti-mystic group could not tolerate any similarity of that sort, which, in their opinion, would damage Paul's uniqueness. The disagreement among biblical scholars can be traced back to more general misgivings about mysticism in European thought. After surveying the reception history of Paul's mysticism, and relying on the ideological critique of religious studies, I argue that the discomfort with a ‘mystical’ Paul may be attributed to the construction of a rational Christian self, where the ‘mystical’ is othered altogether. In addition to a historical reading of Paul in the context of Jewish mysticism, it may be helpful to read him in comparison with Islamic mysticism. Hence some Pauline passages are compared with passages from Sufi literature.
Politische Sprache, Motive und Kritik im Galaterbrief: Eine Spurensuche
Authors: Schreiber Stefan 
Published: September 2022

AbstractThe article analyses the Epistle to the Galatians with regard to textual elements that can be related to language or political performances in the imperium Romanum. To this end, political interpretations in research are first presented and critically discussed: the alleged persecution in Gal 6.12; gods, elements and the calendar in Gal 4.8–10; and the νόμος as Roman law. In a second step, political models of language and thought from the Roman imperial period in the Epistle to the Galatians are considered: the ‘present evil age’ in Gal 1.4; the idea of a new world-view through the concepts of the ‘Gospel’ and the ‘Son of God’; the cross of Christ as a break from the world; the turn of the ages in Gal 4.1–7; the new community according to Gal 3.28. A conclusion summarises the resulting new world-view and its consequences.
Market concentration and the healthiness of packaged food and non-alcoholic beverage sales across the European single market
Authors: Van Dam Iris , Allais Olivier , Vandevijvere Stefanie 
Published: September 2022

AbstractObjective:To assess the relationship between market concentration and diversity, as indicators of market structure, and the healthiness of food and beverage sales across Europe.Design:Market share (MS) data per country were used to calculate market concentration, assessed by the four-firm concentration ratio and market diversity, and by the number of companies with ≥1 % MS and the number of companies uniquely present in one European country. The healthiness of food sales was assessed by applying the NOVA classification (level of processing). Simple and multiple linear regressions were performed to assess the relationship between market concentration, diversity and the healthiness of food and beverage sales.Setting:The European single market.Participants:The twenty-seven European single market member states for which Euromonitor sales data were available at the most fine-grained Euromonitor packaged food and non-alcoholic beverage product subcategory level.Results:Increased market concentration with a country and a product category fixed effect significantly predicted increased sales of ultra-processed packaged food products. There was insufficient data variability in the level of processing of non-alcoholic beverage product categories to formulate conclusions for non-alcoholic beverages. Increased market diversity in turn significantly predicted reduced country-level sales of ultra-processed products.Conclusions:The results indicated a relationship between market structure and the healthiness of packed food products sold on the European market. However, more research with detailed nutritional data is warranted to document and quantify this interaction.
10Be exposure age dating of Late Quaternary relative sea level changes and deglaciation of W Jura and NE Islay, Scottish Inner Hebrides
Authors: DAWSON Alastair G. , BISHOP Paul , HANSOM James , FABEL Derek 
Published: September 2022

ABSTRACTNew 10Be exposure age dating and geomorphological mapping of emerged shoreline features in W Jura and NE Islay throw new light on the regional pattern of ice sheet deglaciation and late-glacial relative sea level change. We conclude that the oldest and highest emerged shorelines in this area were produced ~15.7–16.3 ka, shortly after ice sheet deglaciation ~16.5 ka. It is envisaged that the first incursion of marine waters into coastal areas took place close to a former ice sheet margin that oscillated in position across this part of the Scottish Inner Hebrides. The first evidence of late-glacial marine sedimentation following deglaciation consists of emerged marine terrace fragments and unvegetated gravel beach ridges, the former represented by a prominent glacio-isostatically tilted shoreline that declines in altitude NE to SW, from ~40 m above ordnance datum (OD) in NW Jura to ~19 m OD in central Islay. In W Jura, north of Loch Tarbert, spectacular staircases of up to 55 unvegetated gravel beach ridges were formed shortly after regional deglaciation, possibly within 1 ka. A preliminary estimate of the average rate of relative sea level lowering across W Jura between deglaciation and the Younger Dryas is in the order of ~7 mmyr−1. Geomorphological evidence from Shian Bay, W Jura, indicates a truncation of the late-glacial beach ridge staircases by a large 480-m-long beach ridge (the Colonsay Ridge) at ~14.9 ka, when former relative sea level was at ~18 m OD. This ridge may represent the product of either a stillstand in the progressive lowering of relative sea level during the late-glacial or a reversal. This raises the intriguing possibility of an association between ridge formation and the timing of the well-established global meltwater pulse 1A between ~14.65 and ~14.8 ka.